FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Longland, WS Ostoja, SM AF Longland, William S. Ostoja, Steven M. TI Ecosystem Services from Keystone Species: Diversionary Seeding and Seed-Caching Desert Rodents Can Enhance Indian Ricegrass Seedling Establishment SO RESTORATION ECOLOGY LA English DT Article DE Achnatherum hymenoides; animal-mediated seed dispersal; Dipodomys merriami; Great Basin; heteromyid rodents; kangaroo rats; passive restoration; scatterhoarding ID ASSOCIATIONAL RESISTANCE; ORYZOPSIS-HYMENOIDES; HETEROMYID RODENTS; FOREST RESTORATION; DISPERSAL; RECRUITMENT; BIRDS; TREES; PREFERENCES; POPULATIONS AB Seeds of Indian ricegrass (Achnatherum hymenoides), a native bunchgrass common to sandy soils on arid western rangelands, are naturally dispersed by seed-caching rodent species, particularly Dipodomys spp. (kangaroo rats). These animals cache large quantities of seeds when mature seeds are available on or beneath plants and recover most of their caches for consumption during the remainder of the year. Unrecovered seeds in caches account for the vast majority of Indian ricegrass seedling recruitment. We applied three different densities of white millet (Panicum miliaceum) seeds as diversionary foods to plots at three Great Basin study sites in an attempt to reduce rodents' over-winter cache recovery so that more Indian ricegrass seeds would remain in soil seedbanks and potentially establish new seedlings. One year after diversionary seed application, a moderate level of Indian ricegrass seedling recruitment occurred at two of our study sites in western Nevada, although there was no recruitment at the third site in eastern California. At both Nevada sites, the number of Indian ricegrass seedlings sampled along transects was significantly greater on all plots treated with diversionary seeds than on non-seeded control plots. However, the density of diversionary seeds applied to plots had a marginally non-significant effect on seedling recruitment, and it was not correlated with recruitment patterns among plots. Results suggest that application of a diversionary seed type that is preferred by seed-caching rodents provides a promising passive restoration strategy for target plant species that are dispersed by these rodents. C1 [Longland, William S.] ARS, USDA, Reno, NV 89512 USA. [Ostoja, Steven M.] US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, Oakhurst, CA 93644 USA. RP Longland, WS (reprint author), ARS, USDA, 920 Valley Rd, Reno, NV 89512 USA. EM longland@unr.edu NR 38 TC 7 Z9 9 U1 3 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 J9 RESTOR ECOL JI Restor. Ecol. PD MAR PY 2013 VL 21 IS 2 BP 285 EP 291 DI 10.1111/j.1526-100X.2012.00895.x PG 7 WC Ecology SC Environmental Sciences & Ecology GA 105MN UT WOS:000316075000022 ER PT J AU Busch, DS Sheer, M Burnett, K Mcelhany, P Cooney, T AF Busch, D. Shallin Sheer, Mindi Burnett, Kelly Mcelhany, Paul Cooney, Tom TI LANDSCAPE-LEVEL MODEL TO PREDICT SPAWNING HABITAT FOR LOWER COLUMBIA RIVER FALL CHINOOK SALMON (ONCORHYNCHUS TSHAWYTSCHA) SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE Chinook salmon; habitat modeling; intrinsic potential; digital elevation model; Lower Columbia River (USA) ID DIGITAL ELEVATION MODELS; CHANNEL; BASINS; UNCERTAINTY; ECOSYSTEMS; SELECTION; STREAMS; OREGON; REACH AB We developed an intrinsic potential (IP) model to estimate the potential of streams to provide habitat for spawning fall Chinook salmon (Oncorhynchus tshawytscha) in the Lower Columbia River evolutionarily significant unit. This evolutionarily significant unit is a threatened species, and both fish abundance and distribution are reduced from historical levels. The IP model focuses on geomorphic conditions that lead to the development of a habitat that fish use and includes three geomorphic channel parameters: confinement, width and gradient. We found that the amount of potential habitat for each population does not correlate with current, depressed, total population abundance. However, reaches currently used by spawners have high IP, and IP model results correlate well with results from the complex Ecosystem Diagnosis and Treatment model. A disproportionately large amount of habitat with the best potential is currently inaccessible to fish because of anthropogenic barriers. Sensitivity analyses indicate that uncertainty in the relationship between channel width and habitat suitability has the largest influence on model results and that model form influences model results more for some populations than for others. Published in 2011 by John Wiley & Sons, Ltd. C1 [Busch, D. Shallin; Sheer, Mindi; Mcelhany, Paul] Natl Ocean & Atmospher Adm, NW Fisheries Sci Ctr, Seattle, WA 98112 USA. [Burnett, Kelly] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Cooney, Tom] Natl Ocean & Atmospher Adm, NW Fisheries Sci Ctr, Portland, OR 97232 USA. RP Busch, DS (reprint author), Natl Ocean & Atmospher Adm, NW Fisheries Sci Ctr, 2725 Montlake Blvd E, Seattle, WA 98112 USA. EM Shallin.Busch@noaa.gov FU National Research Council FX T. Beechie, H. Imaki, J. Myers, D. Rawding and the participants of the November 2008 State of the IP workshop in Portland, Oregon, contributed ideas helpful in the development of this model and manuscript. Greg Blair of IFC International, Inc., graciously provided the EDT output used in this study. DSB was supported by a National Research Council post-doctoral fellowship. NR 56 TC 5 Z9 5 U1 1 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD MAR PY 2013 VL 29 IS 3 BP 297 EP 312 DI 10.1002/rra.1597 PG 16 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 108BY UT WOS:000316267200003 ER PT J AU Shields, FD Lizotte, RE Knight, SS AF Shields, F. Douglas, Jr. Lizotte, Richard E., Jr. Knight, Scott S. TI SPATIAL AND TEMPORAL WATER QUALITY VARIABILITY IN AQUATIC HABITATS OF A CULTIVATED FLOODPLAIN SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE water quality; floodplains; agriculture; fish; nitrogen; phosphorus; sediment; hypoxia; restoration; rivers; connectivity; backwaters; wetlands ID YAZOO RIVER-BASIN; DISSOLVED-OXYGEN; OXBOW LAKE; MACROINVERTEBRATE ASSEMBLAGES; MISSISSIPPI RIVER; FISH ASSEMBLAGES; STREAMS; CONNECTIVITY; RESTORATION; COMMUNITY AB The floodplains of lowland rivers contain diverse aquatic habitats that provide valuable ecosystem services but are perturbed when intensively cultivated. Hydrologic, water chemistry and biological (fish) conditions in five aquatic habitats along the Coldwater River, Mississippi, were measured for more than 4years: the river, two severed meanders that functioned as backwaters, a managed wetland and an ephemeral channel draining cultivated fields. Off-channel habitats were connected to downstream regions 0.10% to 32% of the dry season and 24% to 67% of the wet season. The median temperatures for the five monitored sites ranged from 18 degrees C to 23 degrees C, the median total solids concentration for all sites was 135mg L1, the median total phosphorus was 0.29mg L1 and the median total nitrogen was 1.56mg L1. Chemical and physical water quality displayed strong seasonal differences between the wet winter/spring and the dry summer/fall periods so that temporal variation consisted of gradual seasonal trends superimposed on strong diurnal variations. All off-channel habitats exhibited periods of hypoxia and temperatures >30 degrees C during the dry season. Between-site gradients of water and habitat quality were strongly coupled to water depth and runoff loading. The rehabilitation of one backwater by increasing water depth and diverting agricultural runoff was associated with improved water quality and fish species richness relative to an adjacent untreated backwater. The diversion of polluted runoff and the use of water control structures to maintain greater water depth were observed to be effective management tools, but the former reduces the water supply to habitats that tend to dry up and the latter reduces connectivity. Published in 2011 by John Wiley & Sons, Ltd. C1 [Shields, F. Douglas, Jr.; Lizotte, Richard E., Jr.; Knight, Scott S.] ARS, USDA, Oxford, MS 38655 USA. RP Shields, FD (reprint author), ARS, USDA, POB 1157, Oxford, MS 38655 USA. EM doug.shields@ars.usda.gov NR 82 TC 4 Z9 4 U1 7 U2 84 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 J9 RIVER RES APPL JI River Res. Appl. PD MAR PY 2013 VL 29 IS 3 BP 313 EP 329 DI 10.1002/rra.1596 PG 17 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 108BY UT WOS:000316267200004 ER PT J AU Koontz, L Hoag, D DeLong, D AF Koontz, Lynne Hoag, Dana DeLong, Don TI Disparate Stakeholder Management: The Case of Elk and Bison Feeding in Southern Greater Yellowstone SO SOCIETY & NATURAL RESOURCES LA English DT Article ID ANALYTIC HIERARCHY PROCESS; POLITICAL-ECONOMY; PREFERENCES AB For resource decisions to make the most possible progress toward achieving agency mandates, managers must work with stakeholders and may need to at least partially accommodate some of their key underlying interests. To accommodate stakeholder interests, while also substantively working toward fulfilling legal mandates, managers must understand the sociopolitical factors that influence the decision-making process. We coin the phrase disparate stakeholder management (DSM) to describe situations with disparate stakeholders and disparate management solutions. A DSM approach (DSMA) requires decision makers to combine concepts from many sciences, thus releasing them from disciplinary bonds that often constrain innovation and effectiveness. We combined three distinct approaches to develop a DSMA that assisted in developing a comprehensive range of elk and bison management alternatives in the Southern Greater Yellowstone Area. The DSMA illustrated the extent of compromise between meeting legal agency mandates and accommodating the preferences of certain stakeholder groups. C1 [Koontz, Lynne] US Geol Survey, Ft Collins, CO 80526 USA. [Hoag, Dana] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [DeLong, Don] US Forest Serv, USDA, Afton, WY USA. RP Koontz, L (reprint author), US Geol Survey, 2150 Ctr Ave,Bldg C, Ft Collins, CO 80526 USA. EM koontzl@usgs.gov NR 18 TC 0 Z9 0 U1 2 U2 12 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0894-1920 J9 SOC NATUR RESOUR JI Soc. Nat. Resour. PD MAR 1 PY 2013 VL 26 IS 3 BP 339 EP 355 DI 10.1080/08941920.2012.701371 PG 17 WC Environmental Studies; Planning & Development; Sociology SC Environmental Sciences & Ecology; Public Administration; Sociology GA 100DP UT WOS:000315676300007 ER PT J AU Keyser, JD AF Keyser, James D. TI Giving Voice to the Past: Developing the Plains Biographic Rock Art Lexicon SO TIME & MIND-THE JOURNAL OF ARCHAEOLOGY CONSCIOUSNESS AND CULTURE LA English DT Article C1 [Keyser, James D.] US Forest Serv, Washington, DC USA. EM jkeyserfs@comcast.net NR 21 TC 0 Z9 0 U1 0 U2 0 PU BERG PUBL PI LONDON PA BLOOMSBURY PLC, 50 BEDFORD SQ, LONDON, WC1B 3DP, ENGLAND SN 1751-696X J9 TIME MIND JI Time Mind PD MAR PY 2013 VL 6 IS 1 SI SI BP 97 EP 103 DI 10.2752/175169713X13518042629333 PG 7 WC Archaeology SC Archaeology GA 099XL UT WOS:000315659100014 ER PT J AU Edwards, J Bopp, A Graves, E Condon, B AF Edwards, J. Bopp, A. Graves, E. Condon, B. TI IN VITRO HEMOSTATIC, HYDROGEN PEROXIDE PRODUCTION AND ELASTASE SEQUESTRATION PROPERTIES OF NONWOVEN ULTRA CLEAN GREIGE COTTON DRESSING SO WOUND REPAIR AND REGENERATION LA English DT Meeting Abstract C1 [Edwards, J.; Bopp, A.; Graves, E.; Condon, B.] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1067-1927 J9 WOUND REPAIR REGEN JI Wound Repair Regen. PD MAR-APR PY 2013 VL 21 IS 2 BP A21 EP A21 PG 1 WC Cell Biology; Dermatology; Medicine, Research & Experimental; Surgery SC Cell Biology; Dermatology; Research & Experimental Medicine; Surgery GA 103KI UT WOS:000315914400032 ER PT J AU Easson, MW Mertens, JA Fortier, C Slopek, R Condon, B Boykin, D Tarver, M AF Easson, Michael W. Mertens, Jeffery A. Fortier, Chanel Slopek, Ryan Condon, Brian Boykin, Debbie Tarver, Matt TI The Ultrasound-Enhanced Bioscouring Performance of Four Polygalacturonase Enzymes Obtained from Rhizopus oryzae SO AATCC REVIEW LA English DT Article DE Bioscouring; Enzymes; Polygalacturonase; Rhizopus oryzae; Spectrophotometry; Ultrasound ID COTTON; PECTIN; EVOLUTION AB Analytical and statistical methods have been developed to measure the ultrasound-enhanced bioscouring performance of milligram quantities of endo- and exo-polygalacturonase enzymes obtained from Rhizopus oryzae fungi. UV-Vis spectrophotometric data and a general linear mixed models procedure indicate that combinations of ultrasound and endo- and exo-polygalacturonase enzymes give significantly different results from control and could be an environmentally-friendly alternative to the present-day caustic method for cotton preparation. Additionally, endo-RPG-2 performed as well as a commercially-available enzyme source. Evidence supports the synergistic relationship between ultrasound and enzyme treatments. C1 [Easson, Michael W.; Mertens, Jeffery A.; Fortier, Chanel; Slopek, Ryan; Condon, Brian; Boykin, Debbie; Tarver, Matt] USDA ARS, Washington, DC USA. RP Easson, MW (reprint author), USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Michael.Easson@ars.usda.gov NR 14 TC 0 Z9 0 U1 1 U2 11 PU AMER ASSOC TEXTILE CHEMISTS COLORISTS PI RES TRIANGLE PK PA PO BOX 12215, RES TRIANGLE PK, NC 27709 USA SN 1532-8813 J9 AATCC REV JI AATCC Rev. PD MAR-APR PY 2013 VL 13 IS 2 BP 51 EP 56 PG 6 WC Chemistry, Applied; Engineering, Chemical; Materials Science, Textiles SC Chemistry; Engineering; Materials Science GA 099YQ UT WOS:000315662300004 ER PT J AU Wang, SL Tuan, F Gale, F Somwaru, A Hansen, J AF Wang, Sun Ling Tuan, Francis Gale, Fred Somwaru, Agapi Hansen, James TI China's regional agricultural productivity growth in 1985-2007: A multilateral comparison SO AGRICULTURAL ECONOMICS LA English DT Article DE China agricultural productivity; Total factor productivity (TFP); Tornqvist-Thiel (TT) index; China agricultural policy; Multilateral comparison ID INDEX NUMBERS; REFORMS; INVESTMENT; LIVESTOCK; DECLINE; OUTPUT AB In this study, we estimate total factor productivity (TFP) growth as well as multilateral TFP index for 25 contiguous China provinces over the 19852007 period. Agricultural output growth for each province was decomposed into TFP growth and input growth, where input growth was further disaggregated into contributions from growth of labor, capital, land, and intermediate goods. Over the study period, TFP growth contributed 2.7 percentage points to output growth annually, which was slightly higher than the input growth contribution of 2.4 percentage points per annum. On average, the annual rate of productivity growth peaked during 19962000, at 5.1%. It slowed in 20002005 to a rate of 3.2% per annum and declined in the most recent years (20052007) to 3.7%. Differences in productivity among regions persisted over the entire period. The tendency toward faster TFP growth in relatively well-off coastal regions may imply a widening of regional inequality. C1 [Wang, Sun Ling; Gale, Fred; Hansen, James] Econ Res Serv, USDA, Washington, DC 20024 USA. [Tuan, Francis] Renmin Univ China, Sch Agr Econ & Rural Dev, Beijing 100872, Peoples R China. [Somwaru, Agapi] East Asia Econ Consulting, Silver Spring, MD 20904 USA. RP Wang, SL (reprint author), Econ Res Serv, USDA, 355 E St SW, Washington, DC 20024 USA. EM slwang@ers.usda.gov NR 52 TC 4 Z9 4 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0169-5150 J9 AGR ECON-BLACKWELL JI Agric. Econ. PD MAR PY 2013 VL 44 IS 2 BP 241 EP 251 DI 10.1111/agec.12008 PG 11 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 100JQ UT WOS:000315695800008 ER PT J AU Wallner, AM Molano-Flores, B Dietrich, CH AF Wallner, Adam M. Molano-Flores, Brenda Dietrich, Christopher H. TI Evaluating hill prairie quality in the Midwestern United States using Auchenorrhyncha (Insecta: Hemiptera) and vascular plants: a case study in implementing grassland conservation planning and management SO BIODIVERSITY AND CONSERVATION LA English DT Article DE Grassland Auchenorrhyncha; Grassland vegetation; Insect-plant interactions; Grassland management; Hill prairie ID TALLGRASS PRAIRIE; CICADELLIDAE; BONFERRONI; RESERVES; LEAFHOPPERS; COMMUNITIES; ASSEMBLAGES; ARTHROPODS; FIRE AB In this study a habitat quality index based on Auchenorrhyncha (Insecta: Hemiptera) species composition was used to investigate the index's ability in discriminating hill prairie quality along a gradient of disturbance, based on the Illinois Natural Areas Inventory grading criteria; whether this index differs from other vegetation-based measures of quality; and examine the relationships between Auchenorrhyncha and vegetation integrity and diversity. Auchenorrhyncha and vascular plants were sampled from 14 Illinois glacial-drift hill prairies representing a range in quality. Insects and plants were sampled from late July through August, 2007 and analyzed using univariate and multivariate analyses. Results from this study showed that Floristic Quality Index, Auchenorrhyncha Quality Index, as well as other Auchenorrhyncha and plant diversity and integrity index values are greater in high, followed by mid, then low quality hill prairie remnants. Also, these analyses showed that perennial C4 grasses are strongly associated with prairie Auchenorrhyncha. These data suggest that judicious used of prescribed burning or brush removal may be needed to prevent woody-encroachment from eliminating prairie vegetation and Auchenorrhyncha on low quality sites; and restoration of perennial C4 grasses on low quality sites are needed to support more prairie Auchenorrhyncha fauna. Reintroductions of conservative (i.e., prairie-dependent and fire-sensitive) Auchenorrhyncha may also be needed to improve Auchenorrhyncha integrity on mid and low quality sites but these reintroductions need to be used in combination with reduced burn management or the inclusion of unburned refugia to reduce the extirpation of these fire-sensitive insects. C1 [Wallner, Adam M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Molano-Flores, Brenda; Dietrich, Christopher H.] Illinois Nat Hist Survey, Champaign, IL 61820 USA. RP Wallner, AM (reprint author), USDA, APHIS, PPQ, 6302 NW 36th St, Miami, FL 33122 USA. EM wallner77@gmail.com; molano1@illinois.edu; dietrich@inhs.illinois.edu RI Dietrich, Christopher/A-9169-2016 OI Dietrich, Christopher/0000-0003-4005-4305 FU Illinois Department of Natural Resources Large Wildlife Grant FX This research would not have been possible without the help from botanists at the Illinois Natural History, who include James Ellis, Connie Carroll-Cunningham, David Ketzner, Paul Marcum, Michael Murphy, Clark Danderson, and Rick Phillippe. We would also like to thank Michael Miller from the Illinois Department of Natural Resources and Angella Moorehouse from Illinois Nature Preserves Commission for giving us access to the sites visited in this study. We are also grateful to Bryan Cross for providing us with assistance in the field. This project was funded by the Illinois Department of Natural Resources Large Wildlife Grant (2007). We thank Drs. Lawrence Hanks, Andrew Suarez, and John Taft for helpful comments on this manuscript. NR 61 TC 3 Z9 3 U1 2 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3115 EI 1572-9710 J9 BIODIVERS CONSERV JI Biodivers. Conserv. PD MAR PY 2013 VL 22 IS 3 BP 615 EP 637 DI 10.1007/s10531-012-0431-y PG 23 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 097QO UT WOS:000315488800004 ER PT J AU Pardo, LH Semaoune, P Schaberg, PG Eagar, C Sebilo, M AF Pardo, L. H. Semaoune, P. Schaberg, P. G. Eagar, C. Sebilo, M. TI Patterns in delta N-15 in roots, stems, and leaves of sugar maple and American beech seedlings, saplings, and mature trees SO BIOGEOCHEMISTRY LA English DT Article; Proceedings Paper CT 4th Conference on the Mechanisms of Organic Matter Stabilization and Destabilization (SOM) - Organic Matter Stabilization and Ecosystem Functions CY SEP 19-23, 2010 CL FRANCE SP Bioemco Lab (Biogeochemistry and Ecol Continental Ecosystems), Soil Organ Matter Grp DE Stable isotopes; Isotopic fractionation; Nitrogen; Species patterns; Northern hardwood forest ID N-15 NATURAL-ABUNDANCE; NORTHERN HARDWOOD FOREST; NITROGEN ISOTOPE COMPOSITION; BROOK-EXPERIMENTAL-FOREST; BRAZILIAN AMAZON BASIN; SPRUCE PICEA-ABIES; SOIL-NITROGEN; HUBBARD-BROOK; MYCORRHIZAL FUNGI; DEPOSITION GRADIENT AB Stable isotopes of nitrogen (N) in plants are increasingly used to evaluate ecosystem N cycling patterns. A basic assumption in this research is that plant delta N-15 reflects the delta N-15 of the N source. Recent evidence suggests that plants may fractionate on uptake, transport, or transformation of N. If the dominant source of plant N is via roots, a difference in delta N-15 by tissue type would suggest fractionation on transport and assimilation of N. In order to evaluate differences between species and plant parts, we measured delta N-15 in root, stem, and leaf tissues of individual sugar maple (Acer saccharum; SM) and American beech (Fagus grandifolia; BE) plants ranging in age from germinants to mature trees at the Hubbard Brook Experimental Forest, New Hampshire (USA). For SM, root delta N-15 > stem delta N-15 > leaf delta N-15; for BE seedlings, root delta N-15 > stem delta N-15 and root delta N-15 > leaf delta N-15. These differences suggest that fractionation occurs during plant transport and assimilation of N. Beech delta N-15 (root, stem, and leaf) was consistently higher than SM delta N-15 for 1-7 year-old seedlings. At one site, we found no differences with age in foliar delta N-15 (range: 4.1-4.8 aEuro degrees) for seedlings, saplings, and trees which suggests that it may be possible to compare foliar delta N-15 of plants of different ages at some sites. However, at another site, foliar and root delta N-15 were higher for trees than 1-2 year-old seedlings. This study suggests that physiological differences in N assimilation and transport processes that differ by species likely control plant delta N-15. C1 [Pardo, L. H.; Schaberg, P. G.] US Forest Serv, USDA, No Res Stn, S Burlington, VT 05403 USA. [Semaoune, P.; Sebilo, M.] Univ Paris 06, UMR Bioemco 7618, Paris, France. [Eagar, C.] US Forest Serv, USDA, NRS, Durham, NH USA. RP Pardo, LH (reprint author), US Forest Serv, USDA, No Res Stn, 705 Spear St, S Burlington, VT 05403 USA. EM lpardo@fs.fed.us NR 76 TC 8 Z9 9 U1 3 U2 60 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAR PY 2013 VL 112 IS 1-3 BP 275 EP 291 DI 10.1007/s10533-012-9724-1 PG 17 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 104TS UT WOS:000316018800020 ER PT J AU Block, CE Knoepp, JD Fraterrigo, JM AF Block, Corinne E. Knoepp, Jennifer D. Fraterrigo, Jennifer M. TI Interactive effects of disturbance and nitrogen availability on phosphorus dynamics of southern Appalachian forests SO BIOGEOCHEMISTRY LA English DT Article; Proceedings Paper CT 4th Conference on the Mechanisms of Organic Matter Stabilization and Destabilization (SOM) - Organic Matter Stabilization and Ecosystem Functions CY SEP 19-23, 2010 CL FRANCE SP Bioemco Lab (Biogeochemistry and Ecol Continental Ecosystems), Soil Organ Matter Grp DE Exotic insects; Hemlock; Phosphorus fractionation; N and P co-limitation ID NORTHERN HARDWOOD FOREST; SOIL-PHOSPHORUS; ATMOSPHERIC DEPOSITION; NUTRIENT AVAILABILITY; ECOSYSTEM PROPERTIES; RAIN-FORESTS; LIMITATION; IMPACTS; CYCLE; SATURATION AB Understanding the main and interactive effects of chronically altered resource availability and disturbance on phosphorus (P) availability is increasingly important in light of the rapid pace at which human activities are altering these processes and potentially introducing P limitation. We measured P pools and fluxes in eighteen mixed forest stands at three elevations (low, mid, high) subjected to increasing atmospheric N deposition, where hemlock (Tsuga canadensis) was absent or declining due to infestation by the exotic hemlock woolly adelgid (Adelges tsugae). While total soil P was similar across the study area, phosphorus fractionation revealed distinct differences in the distribution of soil P fractions as elevation and N availability increased. Soils from high elevation plots where N availability was greatest had 139 % larger organic P pools and 55 % smaller residual and refractory P pools than soils from low elevation plots with less N availability, suggesting that increased N availability has driven the depletion of recalcitrant P pools by stimulating biotic demand and sequestration. These differences in P distribution among fractions influenced how tree mortality affected P dynamics. At high elevations, plots containing declining hemlocks had significantly greater foliar P concentrations and fluxes of P from the forest floor than reference plots at similar elevations, whereas at low and mid-elevations there were no consistent differences between plots. Across all elevation classes, hardwood foliar N:P ratios were lower in plots with declining hemlocks. Collectively, these results suggest that increased N availability enhances bioavailable P, which is sequestered in vegetation until disturbances liberate it. C1 [Block, Corinne E.; Fraterrigo, Jennifer M.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Knoepp, Jennifer D.] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. RP Fraterrigo, JM (reprint author), Univ Illinois, Dept Nat Resources & Environm Sci, 1102 S Goodwin, Urbana, IL 61801 USA. EM jmf@illinois.edu RI Muldoon, Corinne/F-6705-2013 OI Muldoon, Corinne/0000-0002-5235-0155 NR 55 TC 5 Z9 6 U1 2 U2 66 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAR PY 2013 VL 112 IS 1-3 BP 329 EP 342 DI 10.1007/s10533-012-9727-y PG 14 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 104TS UT WOS:000316018800023 ER PT J AU McFarlane, KJ Torn, MS Hanson, PJ Porras, RC Swanston, CW Callaham, MA Guilderson, TP AF McFarlane, Karis J. Torn, Margaret S. Hanson, Paul J. Porras, Rachel C. Swanston, Christopher W. Callaham, Mac A., Jr. Guilderson, Thomas P. TI Comparison of soil organic matter dynamics at five temperate deciduous forests with physical fractionation and radiocarbon measurements SO BIOGEOCHEMISTRY LA English DT Article; Proceedings Paper CT 4th Conference on the Mechanisms of Organic Matter Stabilization and Destabilization (SOM) - Organic Matter Stabilization and Ecosystem Functions CY SEP 19-23, 2010 CL FRANCE SP Bioemco Lab (Biogeochemistry and Ecol Continental Ecosystems), Soil Organ Matter Grp DE 14C; Carbon cycle; Soil carbon; Soil fractionation; Soil fauna; Terrestrial carbon cycle ID MICRO-PIPETTE METHOD; GLOBAL CARBON-CYCLE; BELOW-GROUND CARBON; MINERAL CONTROL; TROPICAL PASTURES; CENTRAL MISSOURI; AGE CALIBRATION; CONIFER FOREST; CLIMATE-CHANGE; C-14 DATA AB Forest soils represent a significant pool for carbon sequestration and storage, but the factors controlling soil carbon cycling are not well constrained. We compared soil carbon dynamics at five broadleaf forests in the Eastern US that vary in climate, soil type, and soil ecology: two sites at the University of Michigan Biological Station (MI-Coarse, sandy; MI-Fine, loamy); Bartlett Experimental Forest (NH-BF); Harvard Forest (MA-HF); and Baskett Wildlife Recreation and Education Area (MO-OZ). We quantified soil carbon stocks and measured bulk soil radiocarbon to at least 60 cm depth. We determined surface (0-15 cm) soil carbon distribution and turnover times in free light (unprotected), occluded light (intra-aggregate), and dense (mineral-associated) soil fractions. Total soil carbon stocks ranged from 55 +/- A 4 to 229 +/- A 42 Mg C ha(-1) and were lowest at MI-Coarse and MO-OZ and highest at MI-Fine and NH-BF. Differences in climate only partly explained differences in soil organic matter C-14 and mean turnover times, which were 75-260 year for free-light fractions, 70-625 year for occluded-light fractions, and 90-480 year for dense fractions. Turnover times were shortest at the warmest site, but longest at the northeastern sites (NH-BF and MA-HF), rather than the coldest sites (MI-Coarse and MI-Fine). Soil texture, mineralogy, drainage, and macrofaunal activity may be at least as important as climate in determining soil carbon dynamics in temperate broadleaf forests. C1 [McFarlane, Karis J.; Guilderson, Thomas P.] Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, Livermore, CA 94551 USA. [Torn, Margaret S.; Porras, Rachel C.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA. [Hanson, Paul J.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Swanston, Christopher W.] US Forest Serv, USDA, No Res Stn, Houghton, MI 49931 USA. [Callaham, Mac A., Jr.] US Forest Serv, USDA, So Res Stn, Houghton, MI 49931 USA. RP McFarlane, KJ (reprint author), Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, 7000 East Ave,L-397, Livermore, CA 94551 USA. EM mcfarlane3@llnl.gov RI Hanson, Paul J./D-8069-2011; Torn, Margaret/D-2305-2015; OI Hanson, Paul J./0000-0001-7293-3561; McFarlane, Karis/0000-0001-6390-7863 NR 84 TC 19 Z9 20 U1 5 U2 128 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAR PY 2013 VL 112 IS 1-3 BP 457 EP 476 DI 10.1007/s10533-012-9740-1 PG 20 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 104TS UT WOS:000316018800031 ER PT J AU Xi, K Chen, XM Capettini, F Falconi, E Yang, RC Helm, JH Holtz, MD Juskiw, P Kumar, K Nyachiro, J Turkington, TK AF Xi, K. Chen, X. M. Capettini, F. Falconi, E. Yang, R. C. Helm, J. H. Holtz, M. D. Juskiw, P. Kumar, K. Nyachiro, J. Turkington, T. K. TI Multivariate analysis of stripe rust assessment and reactions of barley in multi-location nurseries SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Stripe rust of barley; Puccinia striiformis f. sp hordei; screening barley stripe rust ID RESISTANCE; DISEASE; WHEAT AB Xi, K., Chen, X. M., Capettini, F., Falconi, E., Yang, R. C., Helm, J. H., Holtz, M. D., Juskiw, P., Kumar, K., Nyachiro, J. and Turkington, T. K. 2013. Multivariate analysis of stripe rust assessment and reactions of barley in multi-location nurseries. Can. J. Plant Sci. 93: 209-219. A total of 1357 entries, mainly consisting of hulled two-row, hulled six-row and hulless barley, were evaluated in stripe rust nurseries at Toluca, Mexico during 2007, Quito, Ecuador during 2007 and 2008, and Pullman and Mt. Vernon, USA [Pacific Northwest (PNW)] during 2007-2009. Disease screening data for barley stripe rust resistance at multiple locations and seasons were analyzed using factor analysis (FA), principal component analysis (PCA) and analysis of variance (ANOVA). Factor analysis led to the removal of a number of disease assessment variables that had inadequate disease levels or an unsuitable rating scale. The PCA scores revealed that the two-row types of barley were generally more resistant than the six-row and hulless types. ANOVA indicated that the effect of seasonal influence on screening was small, while stripe rust susceptible and resistant barley types were differentiated significantly on mean values averaged on infection type (IT) and percentage diseased leaf area (disease severity, DS) during the 3-yr tests in multiple screening nurseries. The significant correlations in disease assessments between IT and DS suggest that either assessment can be used to replace the other without a significant loss of information regarding barley stripe rust reactions. The first principal component is a useful criterion for assessing stripe rust reactions in barley lines. C1 [Xi, K.; Capettini, F.; Yang, R. C.; Helm, J. H.; Holtz, M. D.; Juskiw, P.; Kumar, K.; Nyachiro, J.] Alberta Agr & Rural Dev, Field Crop Dev Ctr, Lacombe, AB T4L 1W8, Canada. [Chen, X. M.] Washington State Univ, USDA, ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Chen, X. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Turkington, T. K.] Agr & Agri Food Canada, Lacombe Res Ctr, Lacombe, AB T4L 1W1, Canada. RP Xi, K (reprint author), Alberta Agr & Rural Dev, Field Crop Dev Ctr, Lacombe, AB T4L 1W8, Canada. EM kequan.xi@gov.ab.ca FU Alberta Crop Industry Development Fund FX `We thank L. Hoge for managing barley germplasm and Zhiqiu Hu for input in statistical analysis. The funds provided by Alberta Crop Industry Development Fund to conduct the current research are gratefully acknowledged. NR 27 TC 1 Z9 1 U1 0 U2 14 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD MAR PY 2013 VL 93 IS 2 BP 209 EP 219 DI 10.4141/CJPS2012-051 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 105AS UT WOS:000316038600008 ER PT J AU Beaudette, DE Roudier, P O'Geen, AT AF Beaudette, D. E. Roudier, P. O'Geen, A. T. TI Algorithms for quantitative pedology: A toolkit for soil scientists SO COMPUTERS & GEOSCIENCES LA English DT Article DE Soil data; Soil profile; Soil classification; Soil survey; Numerical soil classification; Aggregation of soil data; Visualization ID SAGEBRUSH COMMUNITIES; CLASSIFICATION UNITS; SOUTHEASTERN OREGON; TAXA; TAXONOMY; QUALITY; ARIZONA AB Soils are routinely sampled and characterized according to genetic horizons, resulting in data that are associated with principle dimensions: location (x, y), depth (z), and property space (p). The high dimensionality and grouped nature of this type of data can complicate standard analysis, summarization, and visualization. The "aqp" (algorithms for quantitative pedology) package was designed to support data-driven approaches to common soils-related tasks such as visualization, aggregation, and classification of soil profile collections. In addition, we sought to advance the study of numerical soil classification by building on previously published methods within an extensible and open source framework. Functions in the aqp package have been successfully applied to studies involving several thousand soil profiles. The stable version of the aqp package is hosted by CRAN (http://cran.r-project.org/web/packages/aqp), and the development version is hosted by R-Forge (http://aqp.r-forges-project.org). Published by Elsevier Ltd. C1 [Beaudette, D. E.] USDA NRCS, Sonora, CA 95222 USA. [O'Geen, A. T.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Roudier, P.] Landcare Res, Palmerston North 4442, New Zealand. RP Beaudette, DE (reprint author), USDA NRCS, 19777 Greenley Rd, Sonora, CA 95222 USA. EM debeaudette@ucdavis.edu FU Kearney Foundation of Soil Science FX Several portions of this research were funded by the Kearney Foundation of Soil Science. We would like to thank Dr. Brent Myers, Jay Skovlin, Dr. Randy Dahlgren, and Stephen Roecker for providing thoughtful commentary on several of the ideas presented in this paper. NR 34 TC 25 Z9 25 U1 2 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-3004 J9 COMPUT GEOSCI-UK JI Comput. Geosci. PD MAR PY 2013 VL 52 BP 258 EP 268 DI 10.1016/j.cageo.2012.10.020 PG 11 WC Computer Science, Interdisciplinary Applications; Geosciences, Multidisciplinary SC Computer Science; Geology GA 098OK UT WOS:000315558400028 ER PT J AU Henry, WB Windham, GL Rowe, DE Blanco, MH Murray, SC Williams, WP AF Henry, W. Brien Windham, Gary L. Rowe, Dennis E. Blanco, Michael H. Murray, Seth C. Williams, W. Paul TI Diallel Analysis of Diverse Maize Germplasm Lines for Resistance to Aflatoxin Accumulation SO CROP SCIENCE LA English DT Article ID ASPERGILLUS EAR ROT; CORN HYBRIDS; REGISTRATION; INBREDS; CONTAMINATION; MYCOTOXINS; FLAVUS AB In this study, diverse, novel maize (Zea mays L.) germplasm from the Germplasm Enhancement of Maize (GEM) Project was screened to determine the combining ability and inheritance of ear rot resistance and resistance to aflatoxin accumulation in hybrid combinations. Nine GEM germplasm lines and six known resistant and susceptible inbred lines were selected as parents of a diallel cross that was evaluated over 2 yr at Mississippi State, MS, and 1 yr at College Station, TX. Resistant lines Mp717 and Mp494 each had negative and significant general combining ability effects, and were frequently present as parents in the hybrid combinations with the lowest aflatoxin accumulation. Of the GEM germplasm lines evaluated, GEMN-0130 was the best source of aflatoxin resistance, with significant and negative GCA values at all three site years. The GEM lines exhibiting significant and negative GCA effects for aflatoxin accumulation in at least one site year include two GEM germplasm lines, GEMS-0030 (2 yr) and GEMS-0074 (1 yr), and a nonreleased GEM source derived from the tropical Cuban accession, CUBA117 (1 yr). The GEM germplasm lines significantly contributing to reduced ear rot in at least two out of three environments included CUBA117, GEMS-0030, GEMS-0002, and GEMN-0130. These results demonstrate the GEM Project is a useful and untapped source of diverse germplasm for resistance to Aspergillus flavus ear rot and aflatoxin accumulation. C1 [Henry, W. Brien; Windham, Gary L.; Williams, W. Paul] USDA ARS, Corn Plant Host Resistance Res Unit, Mississippi State, MS 39762 USA. [Rowe, Dennis E.] Mississippi Agr & Forestry Stn, Mississippi State, MS 39762 USA. [Blanco, Michael H.] Iowa State Univ, GEM, Plant Intro Res Unit, USDA ARS, Ames, IA 50011 USA. [Murray, Seth C.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. RP Henry, WB (reprint author), USDA ARS, Corn Plant Host Resistance Res Unit, Dorman 117 Box 9555, Mississippi State, MS 39762 USA. EM BHenry@pss.msstate.edu NR 40 TC 4 Z9 5 U1 0 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 394 EP 402 DI 10.2135/cropsci2012.04.0240 PG 9 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600005 ER PT J AU Klindworth, DL Hareland, GA Elias, EM Xu, SS AF Klindworth, Daryl L. Hareland, Gary A. Elias, Elias M. Xu, Steven S. TI Attempted Compensation for Linkage Drag Affecting Agronomic Characteristics of Durum Wheat 1AS/1DL Translocation Lines SO CROP SCIENCE LA English DT Article ID WEIGHT GLUTENIN SUBUNITS; NEAR-ISOGENIC LINES; RED SPRING WHEAT; RUST RESISTANCE; COMMON WHEAT; ALLELE GLU-D1D; GRAIN PROTEIN; REGISTRATION; QUALITY; CHROMOSOME AB Yield reduction due to linkage drag is a major problem when introgressing alien chromatin into durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.]. Here we report attempts to compensate for yield losses associated with linkage drag in 1AS.1AL-1DL translocation lines of durum wheat. Near-isogenic lines (NILs) were produced by six backcrosses of a translocation stock to cultivars Renville, Rugby, Lebsock, Ben, and Maier. Rugby was found to be composed of two biotypes, with one biotype having high molecular weight (HMW) glutenin subunits 1Bx6+1By8 (Rugby68) and the second biotype having only 1Bx6 (Rugby6), and a set of NILs based on each biotype was produced. Nearisogenic translocation lines (T-lines) and non-translocation lines (N-lines) were yield tested at five North Dakota locations. Non-translocation lines did not significantly differ from their parental cultivars for yield, heading date, height, protein content, test weight, or thousand kernel weight (TKW). All T-lines yielded significantly less than their sibling N-lines although the Lebsock T-lines had less linkage drag than other T-lines. All T-lines had from 5.9 to 11.3% reduction in TKW. The results indicate that genetic background can partially and possibly completely compensate for linkage drag. But for the 1AS.1AL-1DL translocation lines, improved yield will be dependent on improving TKW, and this may be best accomplished by further reducing the size of the alien segment C1 [Klindworth, Daryl L.; Hareland, Gary A.; Xu, Steven S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Elias, Elias M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. RP Klindworth, DL (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM Daryl.Klind-worth@ars.usda.gov FU U.S. Department of Agriculture (USDA) CRIS [5442-22000-033-00D] FX The authors thank Drs. Jae-Bom Ohm and Michael McMullen for critically reviewing the manuscript and Stan Stancyk for technical support. This research was supported by the U.S. Department of Agriculture (USDA) CRIS Project No. 5442-22000-033-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 42 TC 4 Z9 4 U1 1 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 422 EP 429 DI 10.2135/cropsci2012.05.0310 PG 8 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600008 ER PT J AU Bhusal, SJ Jiang, GL Tilmon, KJ Hesler, LS AF Bhusal, Siddhi J. Jiang, Guo-Liang Tilmon, Kelley J. Hesler, Louis S. TI Identification of Soybean Aphid Resistance in Early Maturing Genotypes of Soybean SO CROP SCIENCE LA English DT Article ID SINGLE DOMINANT GENE; HEMIPTERA-APHIDIDAE; GLYCINES; LINES; ANTIXENOSIS; GERMPLASM; HOMOPTERA; JACKSON AB Soybean aphid (SA) (Aphis glycines Matsumura) has been an important pest of soybean [Glycine max (L.) Merr.] in the United States since 2000. Identification and genetic characterization of SA resistance in early maturing soybean germplasm will facilitate development of aphid-resistant cultivars in the northern region. To identify new sources of SA resistance in early maturing soybeans, a total of 334 soybean genotypes including resistant and susceptible checks were tested in the greenhouse and field. Caged (no-choice) and noncaged tests were used in greenhouse screening under artificial inoculation of SA, and field evaluations were performed relying on natural aphid infestation with or without artificial SA inoculation. In the greenhouse, four genotypes (PI 603712, PI 464911, PI 430491, and PI 603432B) of maturity group (MG) 0 or 00 exhibited low levels of aphid colonization similar to resistant checks, with 17 to 52 aphids per plant 2 wk after inoculation, and three genotypes (PI 612759B, PI 200595, and PI 603426D) of MG 0 were moderately resistant. In the field, however, only PI 603712 and PI 430491 exhibited a resistance reaction with fewer than 100 or 100 to 200 aphids per plant at peak infestation. Plant Introduction 603712 was the only genotype that consistently exhibited resistance to SA in all tests-even higher than that of other known sources of SA resistance in the field. This suggests that PI 603712 might be a new source of SA resistance. In addition, the relatively high levels of SA colonization on a Rag1 genotype (PI 548663 or 'Dowling') in greenhouse tests suggest that the colony used in greenhouse tests might be virulent on Rag1 and therefore might be biotype 2. High levels of SA infestation on Rag1 and Rag2 genotypes in field tests also imply that biotypes 2 and 3 may have been present in the eastern South Dakota field. C1 [Bhusal, Siddhi J.; Jiang, Guo-Liang; Tilmon, Kelley J.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Hesler, Louis S.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Jiang, GL (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM guo-liang.jiang@sdstate.edu OI Bhusal, Siddhi/0000-0002-5071-3741 FU USDA-NIFA/CSREES; SDSRPC; USB; USDA-ARS grants; USDA-ARS CRIS FX We are thankful to Tiana Shuster and Devi Kandel for their help in greenhouse experiments. We thank Marci Green, Nick Hall, Philip Rozeboom, and Eric Beckendorf for their help in planting and management. The help from Mukhtar Agoub, Jiaoping Zhang, and Dr. Xianzhi Wang is appreciated. We thank Randy Nelson and Todd Bedford for providing the soybean germplasm. Mark West provided statistical consultation. This research was supported in part by USDA-NIFA/CSREES, SDSRPC, USB, and USDA-ARS grants and by USDA-ARS CRIS Project base funding. NR 41 TC 6 Z9 6 U1 0 U2 16 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 491 EP 499 DI 10.2135/cropsci2012.06.0397 PG 9 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600015 ER PT J AU Casler, MD Smart, AJ AF Casler, Michael D. Smart, Alexander J. TI Plant Mortality and Natural Selection May Increase Biomass Yield in Switchgrass Swards SO CROP SCIENCE LA English DT Article ID WITHIN-FAMILY SELECTION; SEEDLING TILLER NUMBER; BIG BLUESTEM; POPULATIONS; ADAPTATION; ESTABLISHMENT; REGISTRATION; MORPHOLOGY; GROWTH; GRAMA AB Switchgrass (Panicum virgatum L.) is an important candidate for bioenergy feedstock production, prompting significant efforts to increase the number of breeding programs and the output of those programs. The objective of this experiment was to determine the potential utility of natural selection for survivorship in switchgrass swards as a tool for improving efficiency of progeny-test-based recurrent selection programs in switchgrass. One hundred random surviving plants were selected from 5-yr-old plots of six cultivars grown at five locations. Progeny of each population were grown in a four-location field experiment in Illinois and Wisconsin in direct comparison to their parent populations. On average, natural selection for survivorship increased biomass yield by 6.7%. The northernmost (i.e., coldest) selection location had the greatest response among the five locations (13.2%) while the most genetically diverse cultivar had the greatest response among the cultivars (23.5%). Results were highly variable among cultivars and selection locations, but there were no significant negative responses, suggesting that the genetic correlation between survivorship and biomass yield in switchgrass ranges from zero to some positive value. Selection for survivorship within switchgrass sward plots has the potential to improve efficiency of family-based selection methods designed to improve biomass yield potential. C1 [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Smart, Alexander J.] S Dakota State Univ, Dep Nat Resource Management, Brookings, SD 57007 USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM mdcasler@wisc.edu NR 29 TC 6 Z9 6 U1 2 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 500 EP 506 DI 10.2135/cropsci2012.07.0434 PG 7 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600016 ER PT J AU Olmstead, J Casler, MD Brummer, EC AF Olmstead, Julia Casler, Michael D. Brummer, E. Charles TI Genetic Variability for Biofuel Traits in a Circumglobal Reed Canarygrass Collection SO CROP SCIENCE LA English DT Article ID PHALARIS-ARUNDINACEA L.; BIOMASS YIELD; GRASS; QUALITY; SWITCHGRASS; HARVEST; CROPS; POPULATIONS; PERSISTENCE; MANAGEMENT AB Reed canarygrass (Phalaris arundinacea L.), a cool-season perennial forage crop that grows well in cool, wet climates, has potential value as an energy crop. Nevertheless, little breeding effort has gone into its development as an energy crop. We evaluated 94 reed canarygrass accessions for agronomic and quality traits at Ames, IA, and Arlington, WI, in 1999 and 2000. Variation among accessions was observed for all variables. Biomass yield was not correlated with acid detergent fiber (cellulose plus lignin) or with acid detergent lignin, indicating good potential for developing favorable feedstocks for co-firing or for fermentation. Analysis of regional geographic variation as well as principal component analysis showed that phenotypes varied considerably among geographic regions, with much of the variation attributable to differences between Europe and Asia. Except for North American germplasm, European germplasm tended to have higher biomass yield than other germplasm, suggesting that it may be better suited for use in direct breeding programs in the north central United States. Overall, sufficient variation exists among wild and cultivated germplasm to warrant further breeding of reed canarygrass as a biofuel feedstock. C1 [Olmstead, Julia] Inst Agr & Trade Policy, Minneapolis, MN 55404 USA. [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Brummer, E. Charles] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. RP Brummer, EC (reprint author), Samuel Roberts Noble Fdn Inc, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA. EM ecbrummer@noble.org NR 37 TC 3 Z9 3 U1 0 U2 21 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 524 EP 531 DI 10.2135/cropsci2012.01.0003 PG 8 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600019 ER PT J AU Asoro, FG Newell, MA Scott, MP Beavis, WD Jannink, JL AF Asoro, Franco G. Newell, Mark A. Scott, M. Paul Beavis, William D. Jannink, Jean-Luc TI Genome-wide Association Study for Beta-glucan Concentration in Elite North American Oat SO CROP SCIENCE LA English DT Article ID PENALIZED LOGISTIC-REGRESSION; QUANTITATIVE TRAITS; MIXED-MODEL; SELECTION; ARABIDOPSIS; POPULATION; MARKERS; (1,3/1,4)-BETA-D-GLUCANS; RESOURCE; SAMPLES AB Genome-wide association studies (GWAS) can be a useful approach to detect quantitative trait loci (QTL) controlling complex traits in crop plants. Oat (Avena sativa L.) beta-glucan is a soluble dietary fiber and has been shown to have positive health benefits. We report a GWAS involving 446 elite oat breeding lines from North America genotyped with 1005 diversity arrays technology (DArT) markers and with phenotypic data from both historical and balanced 2-yr data. Association analyses accounting for pair-wise relationships and population structure were conducted using single-marker tests and least absolute shrinkage and selection operator (LASSO). Single-marker tests yielded six and 15 significant markers for the historical and balanced data sets, respectively. The LASSO method selected 24 and 37 markers as the most important in explaining beta-glucan concentration for the historical and balanced data sets, respectively. Comparisons of genetic location showed that 15 of the markers in our study were found on the same linkage groups as QTL identified in previous studies. Four of the markers colocalized to within 4 cM of three previously detected QTL, suggesting concordance between QTL detected in our study and previous studies. Two of the significant markers were also adjacent to a beta-glucan candidate gene in the rice (Oryza sativa L.) genome. Our findings suggest that GWAS can be used for QTL detection for the purpose of gene discovery and for marker-assisted selection to improve beta-glucan concentration in elite oat. C1 [Asoro, Franco G.; Newell, Mark A.; Beavis, William D.] Iowa State Univ, Dep Agron, Ames, IA 50011 USA. [Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Dep Agron, Ames, IA 50011 USA. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. EM jeanluc.jannink@ars.usda.gov FU United States Department of Agriculture, National Institute of Food and Agriculture [2008-55301-18746] FX This research was funded by the United States Department of Agriculture, National Institute of Food and Agriculture, Grant 2008-55301-18746. We thank Adrienne Moran Lauter for laboratory work and George Patrick for field work. NR 49 TC 7 Z9 7 U1 5 U2 33 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 542 EP 553 DI 10.2135/cropsci2012.01.0039 PG 12 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600021 ER PT J AU Wang, Y Bethke, PC AF Wang, Yi Bethke, Paul C. TI Effects of Verticillium dahliae Infection on Stem-End Chip Defect Development in Potatoes SO CROP SCIENCE LA English DT Article ID PRIMARY METABOLISM; SOLANUM-TUBEROSUM; MANAGEMENT; WILT; PATHOGEN; DISEASE; L. AB Potato chips are the most popular snack food in the United States with annual retail sales of over US$6 billion. Stem-end chip defect, which is characterized by dark color of the vasculature and surrounding tissues at the tuber stem end portion of fried chips, is an important tuber quality concern for U. S. chip production. The cause of stem-end chip defect is not known. Verticillium wilt, caused by a vascular fungal pathogen Verticillium dahliae Kleb., is a persistent disease of potato (Solanum tuberosum L.) and other vegetables that causes early plant senescence and yield reductions. A 2-yr field trial was conducted to investigate the effects of V. dahliae on stem-end chip defect development and the activity of acid invertase at the apical (bud) and basal (stem) ends of tubers. Our results show that potato plants that were more infected with V. dahliae had a higher incidence of severe stem-end chip defects than plants with less V. dahliae infection. Verticillium dahliae infection of plants was correlated with an upregulation of acid invertase activity and an accumulation of reducing sugars on the stem end of tubers. Reducing sugars give rise to dark-colored defects as a result of pigments produced by the Maillard reaction during frying. C1 [Wang, Yi; Bethke, Paul C.] Univ Wisconsin, Dep Hort, Madison, WI 53706 USA. [Bethke, Paul C.] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Bethke, PC (reprint author), Univ Wisconsin, Dep Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM paul.bethke@ars.usda.gov FU U.S. Potato Board Chip Committee; Univ. of Wisconsin-Madison Graduate School FX The authors thank Dr. D. I. Rouse for sharing expertise on Verticillium dahliae and Drs. S. H. Jansky and D. A. Halterman for reviewing an early draft of this manuscript. Assistance from the staff at the Hancock Agricultural Research Station is gratefully acknowledged. The authors thank Dr. Pedro Uribe for providing soil CFU count data. The authors also thank Frito-Lay for use of cultivars FL1879 and FL2053 in this research. This work was funded in part by grants from the U.S. Potato Board Chip Committee. Funding from the Univ. of Wisconsin-Madison Graduate School for YW is gratefully acknowledged. NR 19 TC 0 Z9 0 U1 1 U2 20 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 595 EP 601 DI 10.2135/cropsci2012.08.0460 PG 7 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600026 ER PT J AU Burns, JC Fisher, DS AF Burns, J. C. Fisher, D. S. TI Steer Intake, Digestion, and Ingestive Behavior of Switchgrass and Alfalfa Hays SO CROP SCIENCE LA English DT Article ID DRY-MATTER INTAKE; NUTRITIVE-VALUE; BEEF-CATTLE; DIGESTIBILITY; KINETICS; BERMUDAGRASS; FORAGE; PERFORMANCE; GAMAGRASS; BLUESTEM AB Perennial, warm-season grasses cut for hay frequently have inadequate crude protein (CP) concentrations for acceptable ruminant performance. This study determined the influence of alfalfa (Medicago sativa L.) as a CP supplement to steers (Bos taurus) fed switchgrass (Panicum virgatum L.) hay harvested at the early-heading stage. Diets of 100% switchgrass (SG) and 100% alfalfa (AL) were included along with 25, 50, and 75% mixtures of the hays making five treatments. Steer dry matter intake (DMI), whole tract dry matter digestibility (DMD), eating behavior, and masticate characteristics were estimated. Steer DMI averaged 1.35 kg 100 kg(-1) body weight (BW) for 100% SG and increased linearly (P < 0.01) to 2.93 kg 100 kg(-1) BW for 100% AL. Also, DMD increased linearly (P < 0.01) from 484 g kg(-1) for 100% SG to 653 g kg(-1) for 100% AL. Concentration of CP was 51 g kg(-1) for SG and increased quadratically (P < 0.01) to 241 g kg(-1) with increasing AL while neutral detergent fiber (NDF) decreased quadratically (P < 0.01) from 792 g kg(-1) for SG to 407 g kg(-1) for AL. Masticate in vitro dry matter disappearance (IVDMD) increased quadratically (P < 0.01) from 509 g kg(-1) for SG to 660 g kg(-1) in AL whereas CP increased linearly (P < 0.01) from 60 g kg(-1) for SG to 230 g kg(-1) in AL. Increasing AL of the as-fed hay linearly decreased (P < 0.01) chews per gram of dry matter, IVDMD, and CP but increased quadratically (P < 0.01) chews per gram of NDF. Steers readily ate the SG: AL combinations; AL could thus provide a source of CP for SG based diets. C1 [Burns, J. C.] N Carolina State Univ, ARS, USDA, Raleigh, NC 27695 USA. [Burns, J. C.] N Carolina State Univ, Dep Crop Sci, Raleigh, NC 27695 USA. [Burns, J. C.] N Carolina State Univ, Dep Anim Sci, Raleigh, NC 27695 USA. RP Burns, JC (reprint author), N Carolina State Univ, ARS, USDA, Raleigh, NC 27695 USA. EM joe_burns@ncsu.edu NR 36 TC 1 Z9 1 U1 0 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 716 EP 723 DI 10.2135/cropsci2012.07.0458 PG 8 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600041 ER PT J AU Castillo, MS Sollenberger, LE Blount, AR Ferrell, JA Williams, MJ Mackowiak, CL AF Castillo, Miguel S. Sollenberger, Lynn E. Blount, Ann R. Ferrell, Jason A. Williams, Mary J. Mackowiak, Cheryl L. TI Strip Planting a Legume into Warm-Season Grass Pasture: Defoliation Effects During the Year of Establishment SO CROP SCIENCE LA English DT Article ID FLORIGRAZE RHIZOMA PEANUT; ARACHIS-GLABRATA-BENTH; PERENNIAL PEANUT; NUTRITIVE-VALUE; REGISTRATION; PERFORMANCE; PERSISTENCE; MANAGEMENT; AUSTRALIA; EMERGENCE AB Novel approaches are needed for overcoming barriers to successful association of herbaceous legumes with grasses in warm-climate pastures and to identify low-cost, long-term solutions to the problem of N limitation in low-input systems. The objective of this experiment was to evaluate defoliation management options during the year of establishment when rhizoma peanut (RP) (Arachis glabrata Benth.) was strip planted into existing bahiagrass (Paspalum notatum Flugge). Treatments were four defoliation strategies: (i) Control (no defoliation of the planted RP strip and adjacent bahiagrass harvested for hay), (ii) Hay Production (RP strip and adjacent bahiagrass harvested for hay every 28 d), (iii) Simulated Continuous Stocking (pastures grazed weekly), and (iv) Rotational Stocking (pastures grazed every 28 d). Simulated Continuous and Rotational Stocking reduced RP canopy cover and frequency of occurrence. Greatest RP cover during the establishment year was achieved in August with 32 and 29% for the Control and Hay Production treatments compared to 5 and 4% for Simulated Continuous and Rotational Stocking, respectively. Spread of RP was least in Simulated Continuous Stocking. Light penetration to the level of RP in the canopy was not a primary driver of RP response because it was greatest for grazed plots where RP performed poorest. Results show that defoliation management during the establishment year is critical and if pastures are defoliated, hay production is the recommended option. C1 [Castillo, Miguel S.; Sollenberger, Lynn E.; Ferrell, Jason A.] Univ Florida, Agron Dep, Gainesville, FL 32611 USA. [Blount, Ann R.; Mackowiak, Cheryl L.] North Florida Res & Educ Ctr, Marianna, FL 32446 USA. [Williams, Mary J.] USDA NRCS, Gainesville, FL 32614 USA. RP Castillo, MS (reprint author), Univ Florida, Agron Dep, Gainesville, FL 32611 USA. EM mcastillo@ufl.edu NR 32 TC 6 Z9 6 U1 2 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR PY 2013 VL 53 IS 2 BP 724 EP 731 DI 10.2135/cropsci2012.08.0485 PG 8 WC Agronomy SC Agriculture GA 099MC UT WOS:000315624600042 ER PT J AU Zheng, DL Heath, LS Ducey, MJ Quayle, B AF Zheng, Daolan Heath, Linda S. Ducey, Mark J. Quayle, Brad TI Assessing Double Counting of Carbon Emissions Between Forest Land-Cover Change and Forest Wildfires: A Case Study in the United States, 1992-2006 SO ECOSYSTEMS LA English DT Article DE forest fire carbon emission; land-cover change; disturbance; burn severity; carbon double counting; relative contribution ID NET ECOSYSTEM PRODUCTION; FIRE; FLUX; DEFORESTATION; ATMOSPHERE; INVENTORY; DATABASE; 1990S AB The relative contributions of double counting of carbon emissions between forest-to-nonforest cover change (FNCC) and forest wildfires are an unknown in estimating net forest carbon exchanges at large scales. This study employed land-cover change maps and forest fire data in the four representative states (Arkansas, California, Minnesota, and Washington) of the US for the period from 1992 to 2006 to evaluate forest carbon double counting effects based on land-cover change map, forest fire data, and USDA Forest Service Forest Inventory and Analysis data. The analyses were conducted at the county level and tallied to state level. Although the effects were small in the two eastern states because of small burned areas and low burn severity, substantial effects were found in the two western states. Carbon double counting was about 10 TgC (teragram 10(12)) in California and 6 TgC in Washington for the period 1992-2006 (at rates of 0.7 and 0.4 TgC per year), or 21.9 and 7.6% relative to total forest carbon emissions through FNCC in the two states, respectively. The effects were 0.2 and 0.1% in Arkansas and Minnesota, respectively. Variation in double counting effects within the states was also much higher in the western states compared with the eastern states. Our results suggested a general pattern that rates and amounts of double counting in forest carbon emissions between FNCC and fires were more evident and substantially different on a west-east dimension than that on a north-south dimension across the conterminous US during the study period. C1 [Zheng, Daolan; Ducey, Mark J.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Heath, Linda S.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Quayle, Brad] US Forest Serv, USDA, Remote Sensing Applicat Ctr, Salt Lake City, UT 84119 USA. RP Zheng, DL (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. EM daolan.zheng@unh.edu RI Ducey, Mark/K-1101-2016 FU USDA Forest Service [05-DG-11242343-074]; UNH [09JV11242305052]; USFS Northern Research Station Work Unit NRS-5 [09JV11242305052] FX Funding support for this study was primarily from the USDA Forest Service, through grant 05-DG-11242343-074 and partly from the Research Joint Venture Agreement between the UNH and USFS Northern Research Station Work Unit NRS-5 (09JV11242305052). The authors thank Christopher Woodall for his valuable comments on the earlier version of this manuscript. NR 38 TC 2 Z9 2 U1 1 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2013 VL 16 IS 2 BP 310 EP 322 DI 10.1007/s10021-012-9616-1 PG 13 WC Ecology SC Environmental Sciences & Ecology GA 098WQ UT WOS:000315580200010 ER PT J AU Broadhurst, CL Bauchan, GR Murphy, CA Tang, YT Pooley, C Davis, AP Chaney, RL AF Broadhurst, C. Leigh Bauchan, Gary R. Murphy, Charles A. Tang, Ye-Tao Pooley, Christopher Davis, Allen P. Chaney, Rufus L. TI Accumulation of zinc and cadmium and localization of zinc in Picris divaricata Vant. SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Hyperaccumulator; Zinc; Cadmium; Phytoremediation; Trichome; Picris ID HYPERACCUMULATOR THLASPI-CAERULESCENS; CELLULAR COMPARTMENTATION; ARABIDOPSIS-HALLERI; MANGANESE TOLERANCE; IRRIGATION WATER; HIGHER-PLANTS; SILICON; SOIL; TRICHOMES; TOXICITY AB Picris divaricata Vant., a plant species native to subtropical China, was recently identified as the first Cd/Zn hyperaccumulator from Asteraceae. P. divaricata was grown from wild collected seed for 4 months in a series of pH adjusted test soils with added Zn levels 0-7000 mg kg(-1) and Cd levels 0-150 mg kg(-1). Plants did not hyperaccumulate Zn (threshold >3000 mu g g(-1)) and weakly hyperaccumulated Cd with little or no dose-response. P. divaricata has multicellular simple trichomes concentrated on the leaf margins and midrib. X-ray analysis showed that Zn was concentrated in larger trichomes and epidermal cells adjacent to the trichome but virtually absent in other leaf tissues. Within the trichomes, Zn was localized in ovate spots around the tips of individual cells. These tips and other locations in the trichome cell contained black electron dense material when examined with transmission electron microscopy, some of which was identified as SiO2. Silicon and Mn were concentrated in the same areas as Zn. Si has been previously associated with alleviating Zn, Mn and Cd toxicity. Our results support this observation and further investigation is warranted. Calcium and P were concentrated in the distal tips of trichomes, similar to patterns previously observed for calcicole plants grown in elevated Ca soils. Overall, nonsecretory trichomes from many plant families may have a common origin as tissues adapted to handle a variety of environmental metals. (C) 2012 Elsevier B.V. All rights reserved. C1 [Broadhurst, C. Leigh; Chaney, Rufus L.] USDA, Environm Management & Byprod Utilizat Lab, Henry A Wallace Agr Res Ctr, Beltsville, MD 20705 USA. [Broadhurst, C. Leigh; Davis, Allen P.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Bauchan, Gary R.; Murphy, Charles A.; Pooley, Christopher] USDA ARS, Electron & Confocal Microscopy Unit, Beltsville, MD USA. [Tang, Ye-Tao] Sun Yat Sen Univ, Sch Environm Sci & Engn, Guangzhou 510275, Guangdong, Peoples R China. RP Broadhurst, CL (reprint author), USDA, Environm Management & Byprod Utilizat Lab, Henry A Wallace Agr Res Ctr, 10300 Baltimore Blvd,Bldg 007, Beltsville, MD 20705 USA. EM leigh.broadhurst@ars.usda.gov RI DAVIS, ALLEN/F-1066-2017 OI DAVIS, ALLEN/0000-0001-7818-1890 NR 42 TC 6 Z9 6 U1 0 U2 79 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD MAR PY 2013 VL 87 BP 1 EP 9 DI 10.1016/j.envexpbot.2012.08.010 PG 9 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA 102IC UT WOS:000315837300001 ER PT J AU Champ, JG Williams, DR Lundy, CM AF Champ, Joseph G. Williams, Daniel R. Lundy, Catherine M. TI An On-line Narrative of Colorado Wilderness: Self-in-"Cybernetic Space" SO ENVIRONMENTAL COMMUNICATION-A JOURNAL OF NATURE AND CULTURE LA English DT Article DE Blog; Narrative; Self; Trip Reports; Wilderness ID PLACE; EXPERIENCE; IDENTITY; SENSE AB The authors consider a new frontier for the study of wilderness recreation experience, an increasingly common form of blog known as online trip reports. Analysis and discussion in this article is the result of collecting and reflecting upon more than 300 trip reports focused on wilderness areas in the state of Colorado. The authors present a case study of one trip report that demonstrates the intersection of self, narrative, wilderness, and new media technology. While the practice of trip reporting is rather uniform across the cases, the analysis of a single exemplary case reveals that the narrative performance can provide a very personal statement of self and the relationship to wilderness. As Internet presence grows exponentially, online trip reports are expected to play a greater and greater role in the experience of wilderness in cybernetic space. C1 [Champ, Joseph G.; Lundy, Catherine M.] Colorado State Univ, Dept Journalism & Tech Commun, Ft Collins, CO 80523 USA. [Williams, Daniel R.] US Forest Serv, USDA, Rocky Mt Res Stn, Washington, DC USA. RP Champ, JG (reprint author), Colorado State Univ, Dept Journalism & Tech Commun, Campus Delivery 1785, Ft Collins, CO 80523 USA. EM joseph.champ@colostate.edu RI Williams, Daniel/D-8114-2011 OI Williams, Daniel/0000-0003-2428-0345 NR 32 TC 4 Z9 4 U1 0 U2 3 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 1752-4032 J9 ENVIRON COMMUN JI Environ. Commun. PD MAR 1 PY 2013 VL 7 IS 1 SI SI BP 131 EP 145 DI 10.1080/17524032.2012.753099 PG 15 WC Communication; Environmental Studies SC Communication; Environmental Sciences & Ecology GA 099XF UT WOS:000315657100009 ER PT J AU Thomas, JF Engeman, RM Tillman, EA Fischer, JW Orzell, SL Glueck, DH Felix, RK Avery, ML AF Thomas, Jacob F. Engeman, Richard M. Tillman, Eric A. Fischer, Justin W. Orzell, Steve L. Glueck, Deborah H. Felix, Rodney K. Avery, Michael L. TI Optimizing line intercept sampling and estimation for feral swine damage levels in ecologically sensitive wetland plant communities SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Environmental sampling; Feral hog; Invasive species; Seepage slope; Sus scrofa; Wet flatwoods; Comparative accuracy ID FLORIDA; OPTIMIZATION; HABITAT AB Ecological sampling can be labor intensive, and logistically impractical in certain environments. We optimize line intercept sampling and compare estimation methods for assessing feral swine damage within fragile wetland ecosystems in Florida. Sensitive wetland sites, and the swine damage within them, were mapped using GPS technology. Evenly spaced parallel transect lines were simulated across a digital map of each site. The length of each transect and total swine damage under each transect were measured and percent swine damage within each site was estimated by two methods. The total length method (TLM) combined all transects as a single long transect, dividing the sum of all damage lengths across all transects by the combined length of all transect lines. The equal weight method (EWM) calculated the damage proportion for each transect line and averaged these proportions across all transects. Estimation was evaluated using transect spacings of 1, 3, 5, 10, 15, and 20 m. Based on relative root mean squared error and relative bias measures, the TLM produced higher quality estimates than EWM at all transect spacings. Estimation quality decreased as transect spacing increased, especially for TLM. Estimation quality also increased as the true proportion of swine damage increased. Diminishing improvements in estimation quality as transect spacings decreased suggested 5 m as an optimal tradeoff between estimation quality and labor. An inter-transect spacing of 5 m with TLM estimation appeared an optimal starting point when designing a plan for estimating swine damage, with practical, logistical, economic considerations determining final design details. C1 [Thomas, Jacob F.; Glueck, Deborah H.] Univ Colorado Denver, Colorado Sch Publ Hlth, Dept Biostat, Aurora, CO 80045 USA. [Engeman, Richard M.; Fischer, Justin W.] USDA APHIS WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Tillman, Eric A.; Felix, Rodney K.; Avery, Michael L.] USDA APHIS WS Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL 32641 USA. [Orzell, Steve L.] Avon Pk AF Range, Avon Pk Afr, FL 33825 USA. RP Engeman, RM (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov FU National Wildlife Research Center [08-7483-0707(IA)]; Avon Park Air Force Range [08-7483-0707(IA)] FX Field research was supported through interagency agreement 08-7483-0707(IA) between the National Wildlife Research Center and the Avon Park Air Force Range. NR 19 TC 4 Z9 4 U1 4 U2 46 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD MAR PY 2013 VL 20 IS 3 BP 1503 EP 1510 DI 10.1007/s11356-012-1004-z PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 096ZB UT WOS:000315442500028 PM 22707203 ER PT J AU Kilkenny, FF Galloway, LF AF Kilkenny, Francis F. Galloway, Laura F. TI ADAPTIVE DIVERGENCE AT THE MARGIN OF AN INVADED RANGE SO EVOLUTION LA English DT Article DE common garden; invasive species; range limits; range expansion; plasticity; local adaptation; genetic drift; countergradient selection ID HONEYSUCKLE LONICERA-JAPONICA; INTRODUCED PLANT-POPULATIONS; LOCAL ADAPTATION; RAPID EVOLUTION; INVASIVE PLANT; LIMITS; DISPERSAL; HISTORY; CLINES; ESTABLISHMENT AB Invasive plant species threaten biological communities globally. However, relatively little is known about how evolutionary processes vary over the course of an invasion. To evaluate the importance of historical and adaptive drivers of range expansion, we compare the performance of North American populations of invasive Lonicera japonica from areas established 100150 years ago, now the southern core of the range, to populations from the northern range margin, established within the last 65 years. Growth and survival of individuals from 17 core and 14 margin populations were compared in common gardens at both regions. After three years, margin plants were larger than core plants regardless of planting region, with 34% more branches and 36% greater biomass. Growth rate was directly related to survival, and margin plants also had 30% greater survival than core plants across both regions. Larger size of individuals from margin populations suggests either that the shorter growing period at the northern margin has selected for more rapid growth or that range expansion has selected for plants with a greater colonizing ability, including rapid establishment and growth. Because this evolution has resulted in enhanced survival and increased growth rate it may drive spread, increasing the likelihood of further invasion. C1 [Kilkenny, Francis F.; Galloway, Laura F.] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA. RP Kilkenny, FF (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM ffkilkenny@fs.fed.us OI Kilkenny, Francis/0000-0003-1916-6468 FU Jeffress Foundation FX We wish to thank the Plant Sciences Farm at the University of Georgia, the University of Mississippi Field Station, the Russell E. Larson Agricultural Research Farm at Pennsylvania State University, and the Pymatuning Laboratory of Ecology for providing field sites. We are also grateful to E. Miller, B. Barringer, K. Burgess, A. Dai, and E. Yoshizuka for help in the field and greenhouse; to University of Virginia for providing greenhouse space; to W. Crannage for keeping the greenhouse running smoothly; to the Jeffress Foundation for funding; and to FFK's committee members and two anonymous reviewers for comments on previous drafts. NR 53 TC 14 Z9 14 U1 4 U2 95 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0014-3820 J9 EVOLUTION JI Evolution PD MAR PY 2013 VL 67 IS 3 BP 722 EP 731 DI 10.1111/j.1558-5646.2012.01829.x PG 10 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 103DO UT WOS:000315894800011 PM 23461323 ER PT J AU Biedermann, PHW Klepzig, KD Taborsky, M Six, DL AF Biedermann, Peter H. W. Klepzig, Kier D. Taborsky, Michael Six, Diana L. TI Abundance and dynamics of filamentous fungi in the complex ambrosia gardens of the primitively eusocial beetle Xyleborinus saxesenii Ratzeburg (Coleoptera: Curculionidae, Scolytinae) SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE ambrosia fungus gardens; insect agriculture; mycangium; mutualism; social behaviour; mycophagy ID GROWING ANTS; BARK BEETLE; SYMBIONTIC INTERRELATIONSHIPS; MICROFUNGAL COMMUNITIES; FERRUGINEUS; AGRICULTURE; MUTUALISM; BACTERIA; ECTOSYMBIONTS; REPRODUCTION AB Insect fungus gardens consist of a community of interacting microorganisms that can have either beneficial or detrimental effects to the farmers. In contrast to fungus-farming ants and termites, the fungal communities of ambrosia beetles and the effects of particular fungal species on the farmers are largely unknown. Here, we used a laboratory rearing technique for studying the filamentous fungal garden community of the ambrosia beetle, Xyleborinus saxesenii, which cultivates fungi in tunnels excavated within dead trees. Raffaelea sulfurea and Fusicolla acetilerea were transmitted in spore-carrying organs by gallery founding females and established first in new gardens. Raffaelea sulfurea had positive effects on egg-laying and larval numbers. Over time, four other fungal species emerged in the gardens. Prevalence of one of them, Paecilomyces variotii, correlated negatively with larval numbers and can be harmful to adults by forming biofilms on their bodies. It also comprised the main portion of garden material removed from galleries by adults. Our data suggest that two mutualistic, several commensalistic and one to two pathogenic filamentous fungi are associated with X.saxesenii. Fungal diversity in gardens of ambrosia beetles appears to be much lower than that in gardens of fungus-culturing ants, which seems to result from essential differences in substrates and behaviours. C1 [Biedermann, Peter H. W.; Taborsky, Michael] Univ Bern, Inst Ecol & Evolut, Dept Behav Ecol, Bern, Switzerland. [Biedermann, Peter H. W.; Klepzig, Kier D.] US Forest Serv, So Res Stn, USDA, Pineville, LA USA. [Klepzig, Kier D.] US Forest Serv, So Res Stn, Asheville, NC USA. [Six, Diana L.] Univ Montana, Coll Forestry & Conservat, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. RP Biedermann, PHW (reprint author), Max Planck Inst Chem Ecol, Res Grp Insect Symbiosis, Hans Knoll Str 8, D-07745 Jena, Germany. EM pbiedermann@ice.mpg.de RI Taborsky, Michael/F-8420-2013; Biedermann, Peter/E-3641-2013 OI Taborsky, Michael/0000-0002-1357-4316; Biedermann, Peter/0000-0003-4234-5659 FU Department of Behavioural Ecology, University of Bern; Southern Research Station, USDA Forest Service; Austrian Academy of Sciences at the Department of Behavioural Ecology, University of Bern; Roche Research Foundation FX We are grateful to Stacy Blomqvist for her help with trapping X. saxesenii in the field and starting the first laboratory galleries. We also thank Eric Ott for subculturing the fungi and for his first trials to identify the species. This manuscript benefitted greatly from comments of Ulrich G. Mueller, Andre Rodrigues, Tabea Turrini and Gerrit Holighaus. The study was supported by a cooperative agreement between the Department of Behavioural Ecology, University of Bern and the Southern Research Station, USDA Forest Service. PHWB is a recipient of a DOC fellowship of the Austrian Academy of Sciences at the Department of Behavioural Ecology, University of Bern, and was partly funded by a fellowship of the Roche Research Foundation. The authors have no conflict of interest to declare. NR 79 TC 11 Z9 11 U1 3 U2 66 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD MAR PY 2013 VL 83 IS 3 BP 711 EP 723 DI 10.1111/1574-6941.12026 PG 13 WC Microbiology SC Microbiology GA 083OL UT WOS:000314474100016 PM 23057948 ER PT J AU Kingsley, DH AF Kingsley, David H. TI High Pressure Processing and its Application to the Challenge of Virus-Contaminated Foods SO FOOD AND ENVIRONMENTAL VIROLOGY LA English DT Review DE High pressure processing; Foodborne viruses; Bivalve shelfish; Produce; Oysters; Clams; Mussels ID HIGH HYDROSTATIC-PRESSURE; HEPATITIS-A VIRUS; NOROVIRUS SURROGATE; FELINE CALICIVIRUS; MURINE NOROVIRUS; UNITED-STATES; NUCLEOTIDE-SEQUENCE; STRAWBERRY PUREE; ENTEROVIRUS RNA; LOW-TEMPERATURE AB High pressure processing (HPP) is an increasingly popular non-thermal food processing technology. Study of HPP's potential to inactivate foodborne viruses has defined general pressure levels required to inactivate hepatitis A virus, norovirus surrogates, and human norovirus itself within foods such as shellfish and produce. The sensitivity of a number of different picornaviruses to HPP is variable. Experiments suggest that HPP inactivates viruses via denaturation of capsid proteins which render the virus incapable of binding to its receptor on the surface of its host cell. Beyond the primary consideration of treatment pressure level, the effects of extending treatment times, temperature of initial pressure application, and matrix composition have been identified as critical parameters for designing HPP inactivation strategies. Research described here can serve as a preliminary guide to whether a current commercial process could be effective against HuNoV or HAV. C1 Delaware State Univ, ARS, USDA, Dover, DE 19901 USA. RP Kingsley, DH (reprint author), Delaware State Univ, ARS, USDA, Dover, DE 19901 USA. EM david.kingsley@ars.usda.gov NR 85 TC 23 Z9 23 U1 2 U2 80 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1867-0334 EI 1867-0342 J9 FOOD ENVIRON VIROL JI Food Environ. Virol. PD MAR PY 2013 VL 5 IS 1 BP 1 EP 12 DI 10.1007/s12560-012-9094-9 PG 12 WC Environmental Sciences; Microbiology; Virology SC Environmental Sciences & Ecology; Microbiology; Virology GA 104VK UT WOS:000316023800001 PM 23412716 ER PT J AU Liu, Z Wang, DX Feng, JH Seiler, GJ Cai, XW Jan, CC AF Liu, Zhao Wang, Dexing Feng, Jiuhuan Seiler, Gerald J. Cai, Xiwen Jan, Chao-Chien TI Diversifying Sunflower Germplasm by Integration and Mapping of a Novel Male Fertility Restoration Gene SO GENETICS LA English DT Article ID CYTOPLASMIC MALE-STERILITY; HELIANTHUS-ANNUUS L.; SEGREGATION DISTORTION LOCI; CULTIVATED SUNFLOWER; LINKAGE MAP; LOPHOPYRUM-ELONGATUM; RESTORER GENE; AGROPYRON-CRISTATUM; MENDELIAN FACTORS; RESISTANCE GENES AB The combination of a single cytoplasmic male-sterile (CMS) PET-1 and the corresponding fertility restoration (Rf) gene Rf(1) is used for commercial hybrid sunflower (Helianthus annuus L., 2n = 34) seed production worldwide. A new CMS line 514A was recently developed with H. tuberosus cytoplasm. However, 33 maintainers and restorers for CMS PET-1 and 20 additional tester lines failed to restore the fertility of CMS 514A. Here, we report the discovery, characterization, and molecular mapping of a novel Rf gene for CMS 514A derived from an amphiploid (Amp H. angustifolius/P 21, 2n = 68). Progeny analysis of the male-fertile (MF) plants (2n = 35) suggested that this gene, designated Rf(6), was located on a single alien chromosome. Genomic in situ hybridization (GISH) indicated that Rf(6) was on a chromosome with a small segment translocation on the long arm in the MF progenies (2n = 34). Rf(6) was mapped to linkage group (LG) 3 of the sunflower SSR map. Eight markers were identified to be linked to this gene, covering a distance of 10.8 cM. Two markers, ORS13 and ORS1114, were only 1.6 cM away from the gene. Severe segregation distortions were observed for both the fertility trait and the linked marker loci, suggesting the possibility of a low frequency of recombination or gamete selection in this region. This study discovered a new CMS/Rf gene system derived from wild species and provided significant insight into the genetic basis of this system. This will diversify the germplasm for sunflower breeding and facilitate understanding of the interaction between the cytoplasm and nuclear genes. C1 [Liu, Zhao; Feng, Jiuhuan; Seiler, Gerald J.; Cai, Xiwen] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Wang, Dexing] Liaoning Acad Agr Sci, Shenyang 110161, Peoples R China. [Jan, Chao-Chien] ARS, USDA, NCSL, Fargo, ND 58102 USA. RP Jan, CC (reprint author), ARS, USDA, NCSL, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM chaochien.jan@ars.usda.gov NR 70 TC 5 Z9 5 U1 5 U2 24 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 J9 GENETICS JI Genetics PD MAR PY 2013 VL 193 IS 3 BP 727 EP U120 DI 10.1534/genetics.112.146092 PG 24 WC Genetics & Heredity SC Genetics & Heredity GA 103MD UT WOS:000315920000005 PM 23307903 ER PT J AU Kasprak, A Magilligan, FJ Nislow, KH Renshaw, CE Snyder, NP Dade, WB AF Kasprak, Alan Magilligan, Francis J. Nislow, Keith H. Renshaw, Carl E. Snyder, Noah P. Dade, W. Brian TI Differentiating the relative importance of land cover change and geomorphic processes on fine sediment sequestration in a logged watershed SO GEOMORPHOLOGY LA English DT Article DE Soil erosion; Embeddedness; Fallout radionuclides; Riparian ID OREGON COAST RANGE; LARGE WOODY DEBRIS; BED MATERIAL WAVES; ATLANTIC SALMON; RIVER-BASIN; SETTLEMENT FOREST; WESTERN CASCADES; MAINE; HABITAT; STREAMS AB Timber harvest often results in accelerated soil erosion and subsequent elevated fine (<2 mm) sediment delivery to channels causing deleterious effects to numerous aquatic species, particularly salmonid fishes. Here we determine, through sediment physical analyses (pebble counts, embeddedness surveys, and interstitial shelter space counts) and geochemical analyses (Be-7 and Pb-210(ex) activities), the amount and timing of delivery of fine sediment currently found on streambeds of the Narraguagus River watershed in coastal Maine. The role of recent timber harvest, documented via aerial photo spatial analysis, on fine sediment delivery is contrasted with the ability of the glacially influenced topography and surficial geology to deliver fine sediment to streams and to influence channel substrate. Results show that of the land use and geomorphic variables examined, only Pb-210(ex) activities were significantly correlated with the amount of upstream harvest (r(2)=0.49). Concurrently, we find that unit stream power (particularly the slope component) explains much of the variability in channel substrate and that slope and stream power are largely influenced by the legacy of Pleistocene glaciation on channel form. Results suggest a conceptual model whereby fine sediment delivery as a result of late twentieth century timber harvest is likely dampened because of the low gradient landscape of coastal Maine. While geochemical tracers indicate recent fine sediment delivery in harvested areas, channels are likely capable of quickly winnowing these fines from the channel bed. These results further suggest that under contemporary land use conditions, the geomorphic and geologic setting represents a first-order control on channel substrate and habitat suitability for salmonid fishes, including federally endangered Atlantic salmon (Salmo solar), in coastal drainages of northeastern Maine. (C) 2012 Elsevier B.V. All rights reserved. C1 [Kasprak, Alan; Renshaw, Carl E.; Dade, W. Brian] Dartmouth Coll, Dept Earth Sci, Hanover, NH 03755 USA. [Magilligan, Francis J.] Dartmouth Coll, Dept Geog, Hanover, NH 03755 USA. [Nislow, Keith H.] US Forest Serv, USDA, No Res Stn, Amherst, MA 01103 USA. [Snyder, Noah P.] Boston Coll, Dept Earth & Environm Sci, Chestnut Hill, MA 02467 USA. RP Kasprak, A (reprint author), Utah State Univ, Watershed Sci Dept, 5210 Old Main Hill, Logan, UT 84322 USA. EM akasprak@aggiemail.usu.edu FU Geological Society of America [9190-09]; National Science Foundation [EAR 0645343, BCS 0724348] FX This research was funded by a graduate student grant from the Geological Society of America (#9190-09). Additional support was provided by the National Science Foundation for lidar data collection (#EAR 0645343) and for laboratory equipment (#BCS 0724348). Rohan Chaudhary, Matthew Siegfried, and Brynne Weeks (Dartmouth College) assisted in field work. Ernie Atkinson and Greg Mackey (Maine DMR) and Jed Wright (USFWS) were instrumental in field logistics, including lodging at the Maine DMR Little Falls Field Station. Robert Hawley (Dartmouth College) provided essential scientific insight. An Hartmann, John Gartner, and Joshua Landis (Dartmouth College) provided laboratory support and technical advice. Anonymous reviewers provided insightful comments which greatly improved the manuscript. NR 64 TC 9 Z9 9 U1 4 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X J9 GEOMORPHOLOGY JI Geomorphology PD MAR 1 PY 2013 VL 185 BP 67 EP 77 DI 10.1016/j.geomorph.2012.12.005 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 099JB UT WOS:000315616200006 ER PT J AU Roman, MO Gatebe, CK Shuai, YM Wang, ZS Gao, F Masek, JG He, T Liang, SL Schaaf, CB AF Roman, Miguel O. Gatebe, Charles K. Shuai, Yanmin Wang, Zhuosen Gao, Feng Masek, Jeffrey G. He, Tao Liang, Shunlin Schaaf, Crystal B. TI Use of In Situ and Airborne Multiangle Data to Assess MODIS- and Landsat-Based Estimates of Directional Reflectance and Albedo SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Biosphere; ecosystems; land surface; remote sensing ID BIDIRECTIONAL REFLECTANCE; SPECTRAL MEASUREMENTS; SURFACE REFLECTANCE; BRDF MODELS; RETRIEVALS; VALIDATION; ALGORITHM; RESOLUTION; PRODUCTS; CONSISTENCY AB The quantification of uncertainty in satellite-derived global surface albedo products is a critical aspect in producing complete, physically consistent, and decadal land property data records for studying ecosystem change. A challenge in validating albedo measurements acquired from space is the ability to overcome the spatial scaling errors that can produce disagreements between satellite and field-measured values. Here, we present the results from an accuracy assessment of MODIS and Landsat-TM albedo retrievals, based on collocated comparisons with tower and airborne Cloud Absorption Radiometer (CAR) measurements collected during the 2007 Cloud and Land Surface Interaction Campaign (CLASIC). The initial focus was on evaluating inter-sensor consistency through comparisons of intrinsic bidirectional reflectance estimates. Local and regional assessments were then performed to obtain estimates of the resulting scaling uncertainties, and to establish the accuracy of albedo reconstructions during extended periods of precipitation. In general, the satellite-derived estimates met the accuracy requirements established for the high-qualityMODIS operational albedos at 500 m (the greater of 0.02 units or +/- 10% of surface measured values). However, results reveal a high degree of variability in the root-mean-square error (RMSE) and bias of MODIS visible (0.3-0.7 mu m) and Landsat-TM shortwave (0.3-5.0 mu m) albedos; where, in some cases, retrieval uncertainties were found to be in excess of 15%. Results suggest that an overall improvement in MODIS shortwave albedo retrieval accuracy of 7.8%, based on comparisons between MODIS and CAR albedos, resulted from the removal of sub-grid scale mismatch errors when directly scaling-up the tower measurements to the MODIS satellite footprint. C1 [Roman, Miguel O.; Gatebe, Charles K.; Shuai, Yanmin; Masek, Jeffrey G.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Gatebe, Charles K.] Univ Space Res Assoc, Columbia, MD 21044 USA. [Shuai, Yanmin] Earth Resources Technol Inc, Laurel, MD 20707 USA. [Wang, Zhuosen; Schaaf, Crystal B.] Univ Massachusetts, Dept Environm Earth & Ocean Sci, Boston, MA 02125 USA. [Wang, Zhuosen; Schaaf, Crystal B.] Boston Univ, Ctr Remote Sensing, Dept Geog & Environm, Boston, MA 02215 USA. [Gao, Feng] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [He, Tao; Liang, Shunlin] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. RP Roman, MO (reprint author), NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. EM Miguel.O.Roman@nasa.gov; charles.k.gatebe@nasa.gov; Yanmin.Shuai@ertcorp.com; wangzhs@bu.edu; Feng.Gao@ars.usda.gov; jeffrey.g.masek@nasa.gov; the@umd.edu; sliang@umd.edu; schaaf@bu.edu RI Masek, Jeffrey/D-7673-2012; Gatebe, Charles/G-7094-2011; Roman, Miguel/D-4764-2012; liang, shunlin/C-2809-2015; He, Tao/H-5130-2012 OI Gatebe, Charles/0000-0001-9261-2239; Roman, Miguel/0000-0003-3953-319X; He, Tao/0000-0003-2079-7988 FU Science Mission Directorate of the National Aeronautics and Space Administration as part of the Earth Observing System, Radiation Sciences Program; Terrestrial Ecology Program; Airborne Sciences Program; NASA [NNX08AF89G, NNX12AL38G]; U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Program [DOE-DE-FG02-06ER64178] FX This work was supported by the Science Mission Directorate of the National Aeronautics and Space Administration as part of the Earth Observing System, Radiation Sciences Program, the Terrestrial Ecology Program, and the Airborne Sciences Program. The work of C. K. Gatebe was supported by NASA Grant NNX08AF89G. The work of C. B. Schaaf was supported by NASA Grant NNX12AL38G and U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Program under Grant DOE-DE-FG02-06ER64178. NR 53 TC 37 Z9 40 U1 3 U2 45 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 EI 1558-0644 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAR PY 2013 VL 51 IS 3 SI SI BP 1393 EP 1404 DI 10.1109/TGRS.2013.2243457 PN 1 PG 12 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 100TI UT WOS:000315725900030 ER PT J AU Mladenova, IE Jackson, TJ Bindlish, R Hensley, S AF Mladenova, Iliana E. Jackson, Thomas J. Bindlish, Rajat Hensley, Scott TI Incidence Angle Normalization of Radar Backscatter Data SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Backscatter; incidence angle effect; incidence angle normalization; Soil Moisture Active Passive (SMAP) ID SURFACE SOIL-MOISTURE; BOREAL FOREST; AIRSAR DATA; ASAR DATA; IMAGES; MODEL; CLASSIFICATION; PERFORMANCE; CANADA; SENSOR AB The National Aeronautics and Space Administration's (NASA) proposed Soil Moisture Active Passive (SMAP) satellite mission (similar to 2014) will include a radar system that will provide L-band multi-polarization backscatter at a constant incidence angle of 40 degrees. During the pre-launch phase of the project, there is a need for observations that will support the radar-based soil moisture algorithm development and validation. A valuable resource for providing these observations is the NASA Jet Propulsion Laboratory Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR). However, SMAP will observe at a constant incidence angle of 40 degrees, and UAVSAR collects data over a wide range of incidence angles (25 degrees-60 degrees). In this investigation, a technique was developed and tested for normalizing UAVSAR data to a constant incidence angle. The approach is based on a histogram matching procedure. The data used to develop and demonstrate this approach were collected as part of the Canadian Soil Moisture Experiment 2010 (CanEx-SM10). Land cover in the region included agriculture and forest. Evaluation was made possible by the acquisition of numerous overlapping UAVSAR flight lines that provided multiple incidence angle observations of the same locations. Actual observations at a 40 degrees incidence angle were compared to the normalized data to assess performance of the normalization technique. An optimum technique should be able to reduce the systematic error (Bias) to 0 dB and to lower the total root mean square error (RMSE) computed after correction to the level of the initial residual error (RMSEres) present in the data set. The normalization approach developed here achieved both of these. Bias caused by the incidence angle variability was minimized to similar to 0 dB, whereas the residual error caused by instrument related random errors and amplitude fluctuations due to ground variability was reduced to approximately 3 dB for agricultural areas and 2.6 dB for forests; these values were consistent with the initial RMSEres estimated using the un-corrected data. The residual error can be reduced further by aggregating the radar observations to a coarser grid spacing. The technique adequately adjusted the backscatter over the full swath width irrespective of the original incidence angle, polarization, and ground conditions (vegetation cover and soil moisture). In addition to providing a basis for fully exploiting UAVSAR (or similar aircraft systems) for SMAP algorithm development and validation, the technique could also be adapted to satellite radar systems. This normalization approach will also be beneficial in terms of reducing the number of flight lines required to cover a study area, which would eventually result in more cost-effective soil moisture field campaigns. C1 [Mladenova, Iliana E.; Jackson, Thomas J.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20740 USA. [Bindlish, Rajat] Sci Syst & Applicat Inc, Lanham, MD 20706 USA. [Hensley, Scott] NASA, Jet Prop Lab, Pasadena, CA 91109 USA. RP Mladenova, IE (reprint author), USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20740 USA. EM Iliana.Mladenova@ars.usda.gov; thomas.jackson@ars.usda.gov; rajat.bindlish@ars.usda.gov; scott.hensley@jpl.nasa.gov NR 38 TC 15 Z9 15 U1 1 U2 24 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAR PY 2013 VL 51 IS 3 BP 1791 EP 1804 DI 10.1109/TGRS.2012.2205264 PN 2 PG 14 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 100TJ UT WOS:000315726100021 ER PT J AU Dao, MC Sen, S Iyer, C Klebenov, D Meydani, SN AF Dao, M. C. Sen, S. Iyer, C. Klebenov, D. Meydani, S. N. TI Obesity during pregnancy and fetal iron status: is Hepcidin the link? SO JOURNAL OF PERINATOLOGY LA English DT Article DE maternal obesity; iron deficiency; inflammation ID INFLAMMATION; DEFICIENCY; HYPOFERREMIA; TRANSCRIPTION; ASTHMA; TERM; IL-6 AB Objective: To ascertain the effect of obesity-related inflammation on maternal and fetal iron status. We hypothesized that obese (Oh) pregnant women would have increased inflammation, hepcidin levels, and that their infants would have impaired iron status compared with lean (Lc) controls. Study Design: Fifteen Ob and fifteen Lc women were recruited in their second trimester of pregnancy. Markers of iron status, inflammation and hepcidin were measured in maternal and cord blood. Student's t-test was used to compare Ob and Lc groups, and Pearson's correlation coefficients were determined between maternal and cord blood values. Result: Maternal C-reactive protein (P<0.01) and hepcidin (P<0.01) were higher, and cord blood iron (P<0.01) was lower in the Ob group. Maternal body mass index (P<0.01) and hepcidin (P<0.05) were negatively correlated with cord blood iron status. Conclusion: Maternal obesity is associated with impaired maternal-fetal iron transfer, potentially through hepcidin upregulation. Journal of Perinatolog (2013) 33, 177-181; doi:10.1038/jp.2012.81; published online 21 June 2012 C1 [Dao, M. C.; Sen, S.; Meydani, S. N.] Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA USA. [Dao, M. C.; Meydani, S. N.] Friedman Sch Nutr Sci & Policy, Boston, MA USA. [Sen, S.] Tufts Med Ctr, Mother Infant Res Inst, Boston, MA USA. [Sen, S.; Klebenov, D.] Tufts Med Ctr, Floating Hosp Children, Div Newborn Med, Boston, MA USA. [Iyer, C.] Tufts Med Ctr, Dept Obstet & Gynecol, Boston, MA USA. [Meydani, S. N.] Sackler Grad Program Immunol, Boston, MA USA. RP Meydani, SN (reprint author), Tufts Univ, JM USDA HNRCA, 711 Washington St, Boston, MA 02111 USA. EM Simin.Meydani@tufts.edu FU USDA [58-1950-7-707]; Tufts Medical Center Research Grant; Natalie V. Zucker Foundation for Women Scholars; Stanley N. Gershoff scholarship; Nutrition and Cardiovascular Disease Training Program [NHLBI T32] FX This research has been supported by: USDA contract # 58-1950-7-707, Tufts Medical Center Research Grant and The Natalie V. Zucker Foundation for Women Scholars, Stanley N. Gershoff scholarship, NHLBI T32 Nutrition and Cardiovascular Disease Training Program. NR 20 TC 21 Z9 22 U1 0 U2 12 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0743-8346 J9 J PERINATOL JI J. Perinatol. PD MAR PY 2013 VL 33 IS 3 BP 177 EP 181 DI 10.1038/jp.2012.81 PG 5 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA 099ZO UT WOS:000315664700003 PM 22722675 ER PT J AU Liu, CK Latona, NP Taylor, MM Latona, RJ AF Liu, Cheng-Kung Latona, Nicholas P. Taylor, Maryann M. Latona, Renee J. TI EFFECTS OF BATING, PICKLING AND CROSSLINKING TREATMENTS ON THE CHARACTERISTICS OF FIBROUS NETWORKS FROM UN-TANNED HIDES SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID LEATHER AB The U.S hides and leather industries are facing many challenges today, such as overcoming relatively high U.S. energy and labor costs; meeting environmental imperatives; quantifying, maintaining, and improving current hides and leather product quality; developing new processes and products; and improving utilization of waste. One of our efforts to address these new challenges is to develop new uses and novel biobased products from hides to improve prospective markets and to secure a viable future for the hides and leather industries. We hypothesize collagen fiber networks derived from un-tanned hides can be utilized to prepare high performance green composites and air filters, of which both have a great market potential. This study focused on understanding the effects of processing steps such as bating, pickling and crosslinking treatments on the morphology and physical properties of the fiber networks derived from un-tanned hides, which will be the starting material for constructing air filters and green composites. Results showed that glutaraldehyde treatment yielded a highly open structure, in which the fibers are well separated from each other. This could be attributed to the action of acids during the pickling step. C1 [Liu, Cheng-Kung; Latona, Nicholas P.; Taylor, Maryann M.; Latona, Renee J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Liu, CK (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM ChengKung.Liu@ars.usda.gov NR 9 TC 5 Z9 5 U1 4 U2 20 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD MAR PY 2013 VL 108 IS 3 BP 79 EP 85 PG 7 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA 104EN UT WOS:000315974700001 ER PT J AU Sanford, JL Shields, VDC Dickens, JC AF Sanford, Jillian L. Shields, Vonnie D. C. Dickens, Joseph C. TI Gustatory receptor neuron responds to DEET and other insect repellents in the yellow-fever mosquito, Aedes aegypti SO NATURWISSENSCHAFTEN LA English DT Article DE Mosquito; Insect repellent; Feeding deterrent; DEET; Aedes aegypti; Gustatory receptor neuron ID CHEMORECEPTORS; SENSITIVITY AB Three gustatory receptor neurons were characterized for contact chemoreceptive sensilla on the labella of female yellow-fever mosquitoes, Aedes aegypti. The neuron with the smallest amplitude spike responded to the feeding deterrent, quinine, as well as N,N-diethyl-3-methylbenzamide and other insect repellents. Two other neurons with differing spikes responded to salt (NaCl) and sucrose. This is the first report of a gustatory receptor neuron specific for insect repellents in mosquitoes and may provide a tool for screening chemicals to discover novel or improved feeding deterrents and repellents for use in the management of arthropod disease vectors. C1 [Sanford, Jillian L.; Dickens, Joseph C.] ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, Henry A Wallace Beltsville Agr Res Ctr,USDA, Beltsville, MD 20705 USA. [Sanford, Jillian L.; Shields, Vonnie D. C.] Towson Univ, Dept Biol Sci, Towson, MD USA. RP Dickens, JC (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, Henry A Wallace Beltsville Agr Res Ctr,USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov FU Deployed War Fighter Protection Research Program FX The authors are grateful to Dr. Daniel Strickman, USDA, ARS, Beltsville, MD, USA and Dr. Richard G. Vogt, University of South Carolina for reviewing the manuscript. This work was supported in part by a grant to JCD from the Deployed War Fighter Protection Research Program. NR 26 TC 21 Z9 21 U1 2 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0028-1042 J9 NATURWISSENSCHAFTEN JI Naturwissenschaften PD MAR PY 2013 VL 100 IS 3 BP 269 EP 273 DI 10.1007/s00114-013-1021-x PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104PI UT WOS:000316006200009 PM 23407786 ER PT J AU Maruthachalam, K Klosterman, SJ Anchieta, A Mou, BQ Subbarao, KV AF Maruthachalam, Karunakaran Klosterman, Steven J. Anchieta, Amy Mou, Beiquan Subbarao, Krishna V. TI Colonization of Spinach by Verticillium dahliae and Effects of Pathogen Localization on the Efficacy of Seed Treatments SO PHYTOPATHOLOGY LA English DT Article ID GREEN FLUORESCENT PROTEIN; REAL-TIME PCR; RUSSET BURBANK POTATOES; DIFFERENTIAL INTERACTIONS; BRASSICA-NAPUS; WILT; LETTUCE; QUANTIFICATION; TRANSMISSION; ROOTS AB Maruthachalam, K., Klosterman, S. J., Anchieta, A., Mou, B., and Subbarao, K. V. 2013. Colonization of spinach by Verticillium dahliae and effects of pathogen localization on the efficacy of seed treatments. Phytopathology 103:268-280. Verticillium wilt on spinach (Spinacia oleracea) is caused by the soilborne fungus Verticillium dahliae. The pathogen is seedborne and transmission through seed is a major concern because of the dispersal of the pathogen to areas where fresh and processing spinach crops are grown in rotation with susceptible crops. Reduction in seedborne inoculum minimizes pathogen spread; therefore, knowledge of pathogen localization in seed is critical to develop methods to reduce seedborne inoculum. Spinach seedlings were inoculated with conidial suspensions of a green fluorescent protein-tagged strain of V dahliae and colonization events were followed through seed production by confocal laser-scanning microscopy. Between 24 to 96 h postinoculation (PI), conidia germinated and formed hyphal colonies on root tips and in root elongation zones. Hyphae colonized root cortical tissues both intra and intercellularly by 2 weeks, and colonized the taproot xylem with abundant mycelia and conidia that led to vascular discoloration coincident with foliar symptom expression by 8 weeks PI. At 10 weeks PI, the xylem of the upper stem, inflorescence, and spinach seed parts, including the pericarp, seed coat, cotyledons, and radicle, had been colonized by the pathogen but not the perisperm (the diploid maternal tissue). Maximum concentration of the fungus was in the seed coat, the outermost layer of the vasculature. Infection of V dahliae in spinach seed was systemic and transmissible to developing seedlings. Additional analyses indicated that fungicide and steam seed treatments reduced detectable levels of the pathogen but did not eliminate the pathogen from the seed. This information will assist in the development of seed treatments that will reduce the seedborne inoculum transmission to crop production fields. C1 [Maruthachalam, Karunakaran; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, US Agr Res Stn, Salinas, CA 93905 USA. [Klosterman, Steven J.; Anchieta, Amy; Mou, Beiquan] ARS, USDA, Salinas, CA USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU California Department of Food and Agriculture; California Leafy Greens Research Program FX This research was supported by funding from the California Department of Food and Agriculture and California Leafy Greens Research Program. We thank R. Marchebout, C. Marchebout, J. Tanaka, and L. Ochoa for their efforts throughout this project; J. Lincoln for technical assistance with the CLSM; Germains Seed Technology; and L. du Toit for contributing fungicide/steam-treated spinach seed. NR 42 TC 7 Z9 10 U1 0 U2 44 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2013 VL 103 IS 3 BP 268 EP 280 DI 10.1094/PHYTO-05-12-0104-R PG 13 WC Plant Sciences SC Plant Sciences GA 098LU UT WOS:000315551600008 PM 23190117 ER PT J AU Lim, HS Lee, MY Moon, JS Moon, JK Yu, YM Cho, IS Bae, H deBoer, M Ju, H Hammond, J Jackson, AO AF Lim, Hyoun-Sub Lee, Mi Yeon Moon, Jae Sun Moon, Jung-Kyung Yu, Yong-Man Cho, In Sook Bae, Hanhong deBoer, Matt Ju, Hojong Hammond, John Jackson, Andrew O. TI Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins SO PLANT PATHOLOGY JOURNAL LA English DT Article DE Barley stripe mosaic virus; Hordeivirus; Latrunculin B; Membrane proliferation; Triple gene block ID N-TERMINAL DOMAIN; ENDOPLASMIC-RETICULUM; TGB1 MOVEMENT; SUBCELLULAR-LOCALIZATION; INTRACELLULAR-TRANSPORT; PLANT-VIRUSES; REQUIREMENTS; INFECTION; TRAFFICKING; EXPRESSION AB Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin filaments, and that TGB1 alone had no obvious effects on actin filament structure. Latrunculin B (LatB) treatments retarded BSMV cell-to-cell movement, disrupted actin filament organization, and dramatically decreased the proportion of paired TGB3 foci appearing at the cell wall (CW). BSMV infection of transgenic plants tagged with GFP-KDEL exhibited membrane proliferation and vesicle formation that were especially evident around the nucleus. Similar membrane proliferation occurred in plants expressing TGB2 and/or TGB3, and DsRed: Talin fluorescence in these plants colocalized with the ER vesicles. TGB3 also associated with the Golgi apparatus and overlapped with cortical vesicles appearing at the cell periphery. Brefeldin A treatments disrupted Golgi and also altered vesicles at the CW, but failed to interfere with TGB CW localization. Our results indicate that actin cytoskeleton interactions are important in BSMV cell-to-cell movement and for CW localization of TGB3. C1 [Lim, Hyoun-Sub; Yu, Yong-Man] Chungnam Natl Univ, Dept Appl Biol, Taejon 305764, South Korea. [Lee, Mi Yeon; Jackson, Andrew O.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Moon, Jae Sun] Korea Res Inst Biosci & Biotechnol, Green Bio Res Ctr, Taejon 305333, South Korea. [Moon, Jung-Kyung] Rural Dev Adm, Natl Inst Crop Sci, Suwon 441707, South Korea. [Cho, In Sook] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Suwon 441440, South Korea. [Bae, Hanhong] Yeungnam Univ, Sch Biotechnol, Gyongsan, South Korea. [deBoer, Matt] Wageningen Univ, Dept Virol, NL-6709 PD Wageningen, Netherlands. [Hammond, John] USDA ARS, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD 20705 USA. RP Jackson, AO (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM andyoj@berkeley.edu FU Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center), Rural Development Administration [PJ 0090332012]; National Research Foundation of Korea (NRF) [2012R1A1A2007417]; U.S. Department of Agriculture [2008-35319-19225] FX We thank Drs. Steven Ruzin and Denise Schichnes for advice and support with microscopy carried out in conjunction with the research, and to the Biological Imaging Facility at University of California-Berkeley for access to the Zeiss LSM 510 confocal microscope used during the research. We also thank Nam-Hai Chua for the DsRed-talin plasmid, Valarian Dolja for the ST-GFP marker, and David Baulcombe for transgenic N. benthamiana seeds expressing GFP-KDEL and TuA-GFP. This work was supported by grants from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ 0090332012), Rural Development Administration, and the National Research Foundation of Korea (NRF No. 2012R1A1A2007417), and by U.S. Department of Agriculture Competitive Research Grant 2008-35319-19225 to AOJ. NR 49 TC 0 Z9 1 U1 1 U2 13 PU KOREAN SOC PLANT PATHOLOGY PI SUWON PA NATIONAL INST AGRICULTURAL SCIENCE & TECHNOLOGY, PLANT PATHOLOGY DIVISION, SUWON, 441-707, SOUTH KOREA SN 1598-2254 J9 PLANT PATHOLOGY J JI Plant Pathol. J. PD MAR PY 2013 VL 29 IS 1 BP 17 EP 30 DI 10.5423/PPJ.OA.09.2012.0144 PG 14 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA 099HN UT WOS:000315612200003 PM 25288925 ER PT J AU Qiang, ZY Hauck, C Mccoy, JA Widrlechner, MP Reddy, MB Murphy, PA Hendrich, S AF Qiang, Zhiyi Hauck, Cathy McCoy, Joe-Ann Widrlechner, Mark P. Reddy, Manju B. Murphy, Patricia A. Hendrich, Suzanne TI Echinacea sanguinea and Echinacea pallida Extracts Stimulate Glucuronidation and Basolateral Transfer of Bauer Alkamides 8 and 10 and Ketone 24 and Inhibit P-glycoprotein Transporter in Caco-2 Cells SO PLANTA MEDICA LA English DT Article DE Echinacea (Asteraceae); alkamides; ketones; permeability; P-glycoprotein; Caco-2 cells ID HUMAN LIVER-MICROSOMES; BETA-GLUCURONIDASE; CYTOTOXIC ACTIVITY; DRUG ABSORPTION; IN-VIVO; PURPUREA; PERMEABILITY; ALKYLAMIDES; METABOLISM; RESISTANCE AB The use of Echinacea as a medicinal herb is prominent in the United States, and many studies have assessed the effectiveness of Echinacea as an immunomodulator. We hypothesized that Bauer alkamides 8, 10, and 11 and ketone 24 were absorbed similarly either as pure compounds or from Echinacea sanguinea and Echinacea pallida ethanol extracts, and that these Echinacea extracts could inhibit the P-glycoprotein transporter in Caco-2 human intestinal epithelial cells. Using HPLC analysis, the permeation rate of Bauer alkamides by passive diffusion across Caco-2 cells corresponded with compound hydrophilicity (alkamide 8 > 10 > 11), independent of the plant extract matrix. Both Echinacea ethanol extracts stimulated apparent glucuronidation and basolateral efflux of glucuronides of alkamides 8 and 10 but not alkamide 11. Bauer ketone 24 was totally metabolized to more hydrophilic metabolites when administered as a single compound, but was also glucuronidated when present in Echinacea extracts. Bauer alkamides 8, 10, and 11 (175230 mu M) and ethanol extracts of E. sanguinea (1 mg/mL, containing similar to 90 mu M total alkamides) and E. pallida (5 mg/mL, containing 285 mu M total alkamides) decreased the efflux of the P-glycoprotein transporter probe calcein-AM from Caco-2 cells. These results suggest that other constituents in these Echinacea extracts facilitated the metabolism and efflux of alkamides and ketones, which might improve therapeutic benefits. Alkamides and Echinacea extracts might be useful in potentiating some chemotherapeutics, which are substrates for the P-glycoprotein transporter. C1 [Qiang, Zhiyi; Hauck, Cathy; McCoy, Joe-Ann; Widrlechner, Mark P.; Murphy, Patricia A.; Hendrich, Suzanne] Iowa State Univ, Ctr Res Bot Dietary Supplements, Ames, IA 50011 USA. [Widrlechner, Mark P.] Iowa State Univ, USDA ARS, North Cent Reg Plant Intro Stn, Dept Agron, Ames, IA 50011 USA. [Widrlechner, Mark P.] Iowa State Univ, USDA ARS, North Cent Reg Plant Intro Stn, Dept Hort, Ames, IA 50011 USA. [Reddy, Manju B.; Hendrich, Suzanne] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. RP Hendrich, S (reprint author), Iowa State Univ, Dept Food Sci & Human Nutr, 220 MacKay, Ames, IA 50011 USA. EM shendric@iastate.edu OI Hendrich, Suzanne/0000-0001-8700-4765 FU National Center for Complementary & Alternative Medicine [P50AT004155] FX The research described herein was supported by Award Number P50AT004155 from the National Center for Complementary & Alternative Medicine. NR 38 TC 2 Z9 2 U1 0 U2 13 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD MAR PY 2013 VL 79 IS 3-4 BP 266 EP 274 DI 10.1055/s-0032-1328198 PG 9 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 103VQ UT WOS:000315948000011 PM 23408271 ER PT J AU Yang, CH Everitt, JH Du, Q Luo, B Chanussot, J AF Yang, Chenghai Everitt, James H. Du, Qian Luo, Bin Chanussot, Jocelyn TI Using High-Resolution Airborne and Satellite Imagery to Assess Crop Growth and Yield Variability for Precision Agriculture SO PROCEEDINGS OF THE IEEE LA English DT Article DE Hyperspectral imagery; image analysis; multispectral imagery; precision agriculture; satellite imagery; yield variability ID GRAIN-SORGHUM YIELD; REMOTE-SENSING IMAGERY; HYPERSPECTRAL DATA; COMPONENT ANALYSIS; MONITOR DATA; COTTON; SITE; REFLECTANCE; VEGETATION; VIDEO AB With increased use of precision agriculture techniques, information concerning within-field crop yield variability is becoming increasingly important for effective crop management. Despite the commercial availability of yield monitors, many crop harvesters are not equipped with them. Moreover, yield monitor data can only be collected at harvest and used for after-season management. On the other hand, remote sensing imagery obtained during the growing season can be used to generate yield maps for both within-season and after-season management. This paper gives an overview on the use of airborne multispectral and hyperspectral imagery and high-resolution satellite imagery for assessing crop growth and yield variability. The methodologies for image acquisition and processing and for the integration and analysis of image and yield data are discussed. Five application examples are provided to illustrate how airborne multispectral and hyperspectral imagery and high-resolution satellite imagery have been used for mapping crop yield variability. Image processing techniques including vegetation indices, unsupervised classification, correlation and regression analysis, principal component analysis, and supervised and unsupervised linear spectral unmixing are used in these examples. Some of the advantages and limitations on the use of different types of remote sensing imagery and analysis techniques for yield mapping are also discussed. C1 [Yang, Chenghai] ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. [Everitt, James H.] ARS, USDA, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Du, Qian] Mississippi State Univ, Dept Elect & Comp Engn, Mississippi State, MS 39762 USA. [Luo, Bin] Wuhan Univ, State Key Lab Informat Engn Surveying Mapping & R, Wuhan 430079, Hubei, Peoples R China. [Chanussot, Jocelyn] Grenoble Inst Technol, Dept Image & Signal, GIPSA Lab, F-38402 Grenoble, France. RP Yang, CH (reprint author), ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. EM chenghai.yang@ars.usda.gov NR 41 TC 21 Z9 21 U1 2 U2 55 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0018-9219 J9 P IEEE JI Proc. IEEE PD MAR PY 2013 VL 101 IS 3 SI SI BP 582 EP 592 DI 10.1109/JPROC.2012.2196249 PG 11 WC Engineering, Electrical & Electronic SC Engineering GA 097NG UT WOS:000315480200004 ER PT J AU Malcomb, NL Wiles, GC AF Malcomb, Nathan L. Wiles, Gregory C. TI Tree-ring-based reconstructions of North American glacier mass balance through the Little Ice Age - Contemporary warming transition SO QUATERNARY RESEARCH LA English DT Article DE Glacier mass balance; Tree rings; Pacific Northwest; Climate change ID GARIBALDI-PROVINCIAL-PARK; COLUMBIA COAST MOUNTAINS; BRITISH-COLUMBIA; PACIFIC-NORTHWEST; CANADIAN-ROCKIES; PAST MILLENNIUM; LATE-HOLOCENE; CLIMATE VARIATIONS; WADDINGTON AREA; WESTERN CANADA AB Glacier mass-balance reconstructions provide a means of placing relatively short observational records into a longer-term context. In western North America, mass-balance records span four to five decades and capture a relatively narrow window of glacial behavior over an interval that was dominated by warming and ablation. We use temperature- and moisture-sensitive tree-ring series to reconstruct annual mass balance for six glaciers in the Pacific Northwest and Alaska. Mass-balance models rely on the climatic sensitivity of tree-ring chronologies and teleconnection patterns in the North Pacific. The reconstructions extend through the mid to latter portions of the Little Ice Age (LIA) and explore the role of climate variability in forcing mass balance across multiple environmental gradients. Synchronous positive mass-balance intervals coincide with regional moraine building and solar minima, whereas differences in LIA glacier behavior are related to synoptic climate forcing. Secular warming in the late 19th century to present corresponds with the only multi-decadal intervals of negative mass balance in all glacier reconstructions. This suggests that contemporary retreat in western North America is unique with respect to the last several centuries and that regional patterns of glacier variability are now dominated by global climate forcing. Published by Elsevier Inc. on behalf of University of Washington. C1 [Malcomb, Nathan L.] US Forest Serv, Portland Forestry Sci Lab, Pacific NW Res Stn, USDA, Portland, OR 97205 USA. [Wiles, Gregory C.] Coll Wooster, Dept Geol, Wooster, OH 44691 USA. RP Malcomb, NL (reprint author), US Forest Serv, Portland Forestry Sci Lab, Pacific NW Res Stn, USDA, 620 SW Main St,Suite 400, Portland, OR 97205 USA. EM nlmalcomb@fs.fed.us; gwiles@wooster.edu FU Henry J. Copeland Fund for Independent Study; NSF [ATM 99-12124, ATM 09-02799]; National Geographic Society; National Park Service FX This study was supported by the Henry J. Copeland Fund for Independent Study. We thank contributors to the International Tree-ring Data Bank, Brian Luckman, and Emma Watson for the tree-ring data used in this study. The development of the Cordova, Water Supply, and the Hawkins Hill tree-ring series were supported by NSF, ATM 99-12124. The Beartrack chronology was supported by funds from the National Geographic Society, by NSF (ATM 09-02799), and the National Park Service. Suggestions and revisions offered by anonymous reviewers greatly improved this manuscript. Lastly, we are extremely grateful for friends and family who offered sustained encouragement through the completion of this manuscript. NR 79 TC 5 Z9 5 U1 2 U2 49 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0033-5894 EI 1096-0287 J9 QUATERNARY RES JI Quat. Res. PD MAR PY 2013 VL 79 IS 2 BP 123 EP 137 DI 10.1016/j.yqres.2012.11.005 PG 15 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 101EJ UT WOS:000315757700003 ER PT J AU Vadas, PA Bolster, CH Good, LW AF Vadas, P. A. Bolster, C. H. Good, L. W. TI Critical evaluation of models used to study agricultural phosphorus and water quality SO SOIL USE AND MANAGEMENT LA English DT Article DE Phosphorus; modelling ID SURFACE-APPLIED MANURES; LEAST-SQUARES ANALYSIS; TALL FESCUE PASTURE; GOODNESS-OF-FIT; POULTRY LITTER; DISSOLVED PHOSPHORUS; SWAT MODEL; RUNOFF PHOSPHORUS; NUTRIENT LOSSES; SOIL-PHOSPHORUS AB There is an increasing demand to evaluate all agricultural systems where nonpoint source phosphorus (P) pollution is a priority. Because experiments cannot evaluate all possible interactions between management and natural processes that impact P in the environment, computer models are necessary. This article uses research examples to discuss issues related to integrating experimental and model development research. Model development often follows an evolution from a perceptual model of qualitative understanding to a conceptual model of equations to a procedural model of computer code. Integrating experiments and models is an efficient way to show if conceptual models are incorrect and design hypothesis-driven experiments to develop alternative models. A model can be perceptually correct but conceptually incorrect, such as some P Indexes in the US. Translation of conceptual equations into procedural model equations can vary between models, such as how the same soil P model varies in EPIC, SWAT and AnnAGNPS. Commonly used P models often do not reflect current science, such as in simulating soil total P and inorganic P sorption and desorption, surface-applied manure and fertilizer and direct P loss in run-off, grazing cattle dung dynamics and contributions to P in run-off, and subsurface P leaching and transport. Thus, models may be applied in situations for which their algorithms are inadequate. Validating models using more than one output parameter, over multiple years and with diverse scenarios can help identify model weaknesses. Ultimately, a framework of integrated experimentation and model development can advance agricultural P science and environmental protection beyond the point that either can achieve alone. C1 [Vadas, P. A.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Bolster, C. H.] USDA ARS, Bowling Green, KY 42104 USA. [Good, L. W.] Univ Wisconsin, Madison, WI 53706 USA. RP Vadas, PA (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM peter.vadas@ars.usda.gov NR 58 TC 6 Z9 6 U1 0 U2 99 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-0032 EI 1475-2743 J9 SOIL USE MANAGE JI Soil Use Manage. PD MAR PY 2013 VL 29 SU 1 SI SI BP 36 EP 44 DI 10.1111/j.1475-2743.2012.00431.x PG 9 WC Soil Science SC Agriculture GA 102EY UT WOS:000315827800005 ER PT J AU Smith, DR Livingston, SJ AF Smith, D. R. Livingston, S. J. TI Managing farmed closed depressional areas using blind inlets to minimize phosphorus and nitrogen losses SO SOIL USE AND MANAGEMENT LA English DT Article DE Blind inlet; drainage water; phosphorus transport; closed depression; tile riser ID NUTRIENT TRANSPORT; LAKE-ERIE; SURFACE; RUNOFF; AGRICULTURE; IOWA AB Closed depressions are sites within a landscape from glacial origin in which runoff water tends to collect because there is no natural outlet. When farmed, this water is often drained via a tile riser, a vertical tube that connects the bottom of the low point of a closed depression with the subsurface tile drainage network. Two field-sized closed depressions (ca. 4ha) and two small catchments (ca. 300ha) were used to test the hypothesis that water quality could be improved by replacing tile risers with blind inlets, ca. 18m2 holes filled to 1m depth so that a high infiltration capacity was attained. At the field scale discharge, sediment and nutrient loads were lower when drained with the blind inlet compared to the tile riser. Late Spring 2010 was much wetter than normal, and increased nutrient loading was observed in both small catchments compared with previous years. In 2010, discharge and total P loading from Catchment AME (control catchment drained with tile risers) increased 417% and 737%, respectively, compared to the mean for the six previous years, whereas in Catchment BME (treatment catchment drained with blind inlets), there was only a 64% and 92% increase, respectively. Results from this study indicate that replacing tile risers with blind inlets to drain closed depressions may be one method to effectively reduce nutrient and sediment loading to Lake Erie and other sensitive water bodies that lie in landscapes formed by Wisconsin, Weichselian or Devensian glaciation. C1 [Smith, D. R.; Livingston, S. J.] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47906 USA. RP Smith, DR (reprint author), ARS, USDA, Natl Soil Eros Res Lab, 275 South Russell St, W Lafayette, IN 47906 USA. EM douglas.r.smith@ars.usda.gov NR 18 TC 10 Z9 10 U1 0 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-0032 J9 SOIL USE MANAGE JI Soil Use Manage. PD MAR PY 2013 VL 29 SU 1 SI SI BP 94 EP 102 DI 10.1111/j.1475-2743.2012.00441.x PG 9 WC Soil Science SC Agriculture GA 102EY UT WOS:000315827800012 ER PT J AU Patindol, J Shih, F Ingber, B Champagne, E Boue, S AF Patindol, James Shih, Frederick Ingber, Bruce Champagne, Elaine Boue, Stephen TI Porous rice powder from the precipitation of gelatinized flour or starch paste with ethanol SO STARCH-STARKE LA English DT Article DE Flour; Precipitation; Pregelatinized powder; Rice; Starch ID EXTRUSION-COOKING; WHEAT-STARCH AB Hot paste obtained by autoclaving (130 degrees C, 25 psi, 2030min) a 57% w/w rice flour or starch slurry was precipitated with ethanol (three extractions) to produce a dry, porous, pregelatinized powder with an average particle size of 75.0 mu m (flour-derived powder) and 41.6 mu m (starch-derived powder). The microstructure of the individual particles was characterized by an interconnecting lattice of irregularly shaped vesicles, and with cavities of varying size and shape. The vesicular network was relatively thinner and finer for the starch-derived products compared with the flour-derived ones. In comparison with native flour and starch, the bulk density of pregelatinized powders decreased; solvent uptake (water, oil, and alcohol), swelling power, and in vitro starch digestibility increased; whereas, gel consistency, freezethaw stability, and AAM content sparingly changed. Changes in morphological and physicochemical properties were generally more evident on the starch-derived products compared with the flour-derived counterparts. To some extent, changes in properties were also affected by severity of the gelatinization treatment (varying slurry concentration and autoclaving duration). The pregelatinized rice products as developed may be useful in food and non-food applications. C1 [Patindol, James; Shih, Frederick; Ingber, Bruce; Champagne, Elaine; Boue, Stephen] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Boue, S (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM steve.boue@ars.usda.gov NR 22 TC 1 Z9 1 U1 2 U2 22 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 0038-9056 J9 STARCH-STARKE JI Starch-Starke PD MAR PY 2013 VL 65 IS 3-4 BP 296 EP 303 DI 10.1002/star.201200134 PG 8 WC Food Science & Technology SC Food Science & Technology GA 102DO UT WOS:000315824000014 ER PT J AU Ridpath, JF Falkenberg, SM Bauermann, FV VanderLey, BL Do, Y Flores, EF Rodman, DM Neill, JD AF Ridpath, Julia F. Falkenberg, Shollie M. Bauermann, Fernando V. VanderLey, Brian L. Do, YoonJung Flores, Eduardo F. Rodman, Dianne M. Neill, John D. TI Comparison of acute infection of calves exposed to a high-virulence or low-virulence bovine viral diarrhea virus or a HoBi-like virus SO AMERICAN JOURNAL OF VETERINARY RESEARCH LA English DT Article ID ATYPICAL PESTIVIRUS; NORTH-AMERICA; ANTIGEN; LESIONS; STRAIN; CATTLE; BVDV; THROMBOCYTOPENIA; DISEASE; TYPE-2 AB Objective-To compare acute infection of cattle exposed to a high-virulence (HV) bovine viral diarrhea virus (BVDV), low-virulence (LV) BVDV, or HoBi-like virus. Animals-24 Holstein bull calves. Procedures-Colostrum-deprived 2- to 4-week-old calves, free of BVDV antigen and antibodies, were allocated into 4 groups (6 calves/group). Calves in 3 groups were exposed to an LV BVDV strain (BVDV2-RS886), an HV BVDV strain (BVDV2-1373), or a HoBi-like virus (D32/00 HoBi), whereas calves in the fourth group were not exposed to a virus but were cohoused with calves exposed to the HoBi-like virus. Circulating WBCs, platelets, rectal temperature, and presence of virus in the blood were monitored. Results-Infection of calves with any of the 3 viruses resulted in reduced numbers of circulating WBCs. Pyrexia was detected in all calves exposed to HV BVDV or LV BVDV but in only 3 of 6 calves exposed to the HoBi-like virus. Diarrhea was observed in 0 of 6 calves exposed to the HoBi-like virus, 2 Of 6 calves exposed to the LV BVDV, and 6 of 6 calves exposed to the HV BVDV. The HoBi-like virus was transmitted from acutely infected calves to naive cohorts. Conclusions and Clinical Relevance-The HoBi-like viruses are an emerging species of pestivirus isolated from water buffalo and cattle in South America, Southeast Asia, and Europe but not from cattle in the United States. Understanding the clinical course of disease caused by HoBi-like pestiviruses will be important for the design of surveillance programs for the United States. (Am J Vet Res 2013;74:438-442) C1 [Ridpath, Julia F.; Falkenberg, Shollie M.; VanderLey, Brian L.; Neill, John D.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Bauermann, Fernando V.; Flores, Eduardo F.] Univ Fed Santa Maria, Virus Sect, Dept Prevent Vet Med, BR-97119900 Santa Maria, RS, Brazil. [Do, YoonJung] Natl Inst Anim Sci, Suwon, South Korea. [Rodman, Dianne M.] USDA, Diagnost Virol Lab, Natl Vet Serv Lab, APHIS, Ames, IA 50010 USA. RP Ridpath, JF (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM julia.ridpath@ars.usda.gov RI flores, eduardo/H-5125-2016 NR 32 TC 23 Z9 25 U1 0 U2 12 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0002-9645 J9 AM J VET RES JI Am. J. Vet. Res. PD MAR PY 2013 VL 74 IS 3 BP 438 EP 442 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 099JG UT WOS:000315616700011 PM 23438120 ER PT J AU Donaldson-Matasci, MC DeGrandi-Hoffman, G Dornhaus, A AF Donaldson-Matasci, Matina C. DeGrandi-Hoffman, Gloria Dornhaus, Anna TI Bigger is better: honeybee colonies as distributed information-gathering systems SO ANIMAL BEHAVIOUR LA English DT Article DE Apis mellifera; collective behaviour; colony size; communication; foraging; honeybee; information; resource distribution; social insect ID APIS-MELLIFERA; FORAGING BEHAVIOR; GROUP-SIZE; BEES; BENEFITS; RECRUITMENT; FORAGERS; DANCE; COMMUNICATION; HYMENOPTERA AB In collectively foraging groups, communication about food resources can play an important role in the organization of the group's activity. For example, the honeybee dance communication system allows colonies to selectively allocate foragers among different floral resources according to their quality. Because larger groups can potentially collect more information than smaller groups, they might benefit more from communication because it allows them to integrate and use that information to coordinate forager activity. Larger groups might also benefit more from communication because it allows them to dominate high-value resources by recruiting large numbers of foragers. By manipulating both colony size and the ability to communicate location information in the dance, we show that larger colonies of honeybees benefit more from communication than do smaller colonies. In fact, colony size and dance communication worked together to improve foraging performance; the estimated net gain per foraging trip was highest in larger colonies with unimpaired communication. These colonies also had the earliest peaks in foraging activity, but not the highest ones. This suggests they may find and recruit to resources more quickly, but not more heavily. The benefits of communication we observed in larger colonies are thus likely a result of more effective information-gathering due to massive parallel search rather than increased competitive ability due to heavy recruitment. (C) 2013 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved. C1 [Donaldson-Matasci, Matina C.; Dornhaus, Anna] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ 85721 USA. [DeGrandi-Hoffman, Gloria] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ USA. RP Donaldson-Matasci, MC (reprint author), Univ Arizona, Dept Ecol & Evolutionary Biol, POB 210088, Tucson, AZ 85721 USA. EM matina@email.arizona.edu FU University of Arizona's Center for Insect Science through a National Institutes of Health (NIH) [1K12GM000708]; National Science Foundation [IOS-0921280, IOS-0841756] FX M.C.D. was funded by the University of Arizona's Center for Insect Science through a National Institutes of Health (NIH) Training Grant (1K12GM000708). A. D. was funded by National Science Foundation grants (IOS-0921280 and IOS-0841756). We thank M. Heitlinger at the Santa Rita Experimental Range Headquarters for logistical support and P. Jenkins at the University of Arizona Herbarium for help with plant species identification. We gratefully acknowledge the fieldwork assistance of N. Matasci, J. Brown, S. Boleyn and J. Henkel. We also thank two anonymous referees for their helpful comments on the manuscript. NR 38 TC 12 Z9 12 U1 1 U2 64 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0003-3472 J9 ANIM BEHAV JI Anim. Behav. PD MAR PY 2013 VL 85 IS 3 BP 585 EP 592 DI 10.1016/j.anbehav.2012.12.020 PG 8 WC Behavioral Sciences; Zoology SC Behavioral Sciences; Zoology GA 101SJ UT WOS:000315794400011 PM 26213412 ER PT J AU Gurtler, JB Douds, DD Dirks, BP Quinlan, JJ Nicholson, AM Phillips, JG Niemira, BA AF Gurtler, Joshua B. Douds, David D., Jr. Dirks, Brian P. Quinlan, Jennifer J. Nicholson, April M. Phillips, John G. Niemira, Brendan A. TI Salmonella and Escherichia coli O157:H7 Survival in Soil and Translocation into Leeks (Allium porrum) as Influenced by an Arbuscular Mycorrhizal Fungus (Glomus intraradices) SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CONTAMINATED IRRIGATION WATER; LISTERIA-MONOCYTOGENES; MEDICAGO-TRUNCATULA; SPINACH PLANTS; TOMATO PLANTS; LETTUCE; INTERNALIZATION; COLONIZATION; ENTERICA; O157-H7 AB A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions of Salmonella or EHEC at ca. 8.2 log CFU/plant into soil. Soil, root, and shoot samples were analyzed for pathogens on days 1, 8, 15, and 22 postinoculation. Initial populations (day 1) were ca. 3.1 and 2.1 log CFU/root, ca. 2.0 and 1.5 log CFU/shoot, and ca. 5.5 and 5.1 CFU/g of soil for Salmonella and EHEC, respectively. Enrichments indicated that at days 8 and 22, only 31% of root samples were positive for EHEC, versus 73% positive for Salmonella. The mean Salmonella level in soil was 3.4 log CFU/g at day 22, while EHEC populations dropped to <= 0.75 log CFU/g by day 15. Overall, Salmonella survived in a greater number of shoot, root, and soil samples, compared with the survival of EHEC. EHEC was not present in Myc- shoots after day 8 (0/16 samples positive); however, EHEC persisted in higher numbers (P = 0.05) in Myc+ shoots (4/16 positive) at days 15 and 22. Salmonella, likewise, survived in statistically higher numbers of Myc+ shoot samples (8/8) at day 8, compared with survival in Myc- shoots (i. e., only 4/8). These results suggest that AM fungi may potentially enhance the survival of E. coli O157:H7 and Salmonella in the stems of growing leek plants. C1 [Gurtler, Joshua B.; Niemira, Brendan A.] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Douds, David D., Jr.] ARS, Mol Characterizat & Foodborne Pathogen Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Dirks, Brian P.] Drexel Univ, Dept Biol, Coll Arts & Sci, Philadelphia, PA 19104 USA. [Quinlan, Jennifer J.] Drexel Univ, Dept Nutr Sci, Coll Nursing & Hlth Profess, Philadelphia, PA 19104 USA. [Nicholson, April M.] Delaware State Univ, Coll Agr & Related Sci, Dover, DE USA. [Phillips, John G.] ARS, USDA, Wyndmoor, PA USA. RP Gurtler, JB (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM joshua.gurtler@ars.usda.gov NR 61 TC 4 Z9 5 U1 1 U2 47 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2013 VL 79 IS 6 BP 1813 EP 1820 DI 10.1128/AEM.02855-12 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 097DP UT WOS:000315454500006 PM 23315740 ER PT J AU Pan, ZL Khir, R Thompson, JF AF Pan, Zhongli Khir, Ragab Thompson, James F. TI Effect of Milling Temperature and Postmilling Cooling Procedures on Rice Milling Quality Appraisals SO CEREAL CHEMISTRY LA English DT Article ID ENVIRONMENTAL-CONDITIONS; MILLED RICE; KERNEL BREAKAGE; ROUGH RICE; TRANSITION AB The objective of this research was to study the effects of different milling conditions and postmilling handling procedures on appraised milling quality of rough rice. Rough rice (M202) with moisture content of 11.5 +/- 0.2% was used for this study. The samples were milled with a McGill number 3 mill under four milling conditions, including normal milling, milling at high temperature, milling with cooling using ice water, and room temperature water. The milled rice samples were cooled in closed and open plastic containers and in open pans with three temperatures: 15, 23, and 35 degrees C. The effects of milling and postmilling conditions on milled rice temperature, moisture loss, cooling rate, single and multiple fissuring rates, total rice yield (TRY), head rice yield (HRY), whiteness index (WI), and total lipid content (TLC) were evaluated. Results showed that high single and multiple fissuring rates and low TRY and HRY were inherent in improper milling and postmilling conditions. Single fissuring rates were 15.9 and 17.6% and multiple fissuring rates were 3.5 and 7.2% for rice samples milled under normal and high-temperature conditions, respectively. Cooling methods that used open containers and pans had more moisture losses and further resulted in lowering appraised milling quality than methods that used closed containers. Low-temperature milling conditions followed by cooling in closed containers significantly reduced single and multiple fissuring rates and improved TRY and HRY by 0.9 and 1.5 percentage points, respectively. The effects of tested milling and postmilling conditions on WI and TLC were not significant. Obtained results constitute valuable information for developing milling and cooling procedures to achieve consistent, accurate, and reliable milling quality appraisals for rough rice. C1 [Pan, Zhongli; Khir, Ragab; Thompson, James F.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] ARS, Proc Foods Res Unit, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Khir, Ragab] Suez Canal Univ, Dept Agr Engn, Fac Agr, Ismailia, Egypt. RP Pan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, 1 Shields Ave, Davis, CA 95616 USA. EM zhongli.pan@ars.usda.gov FU California Rice Research Board; USDA; CDFA; FGIS; Pacific International Rice Mills, Inc. FX The investigators express their appreciation for the partial financial support from the California Rice Research Board and supports received from the USDA, CDFA, FGIS, and Pacific International Rice Mills, Inc. NR 30 TC 1 Z9 1 U1 2 U2 13 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2013 VL 90 IS 2 BP 107 EP 113 DI 10.1094/CCHEM-08-12-0095-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 099TO UT WOS:000315645200003 ER PT J AU Graybosch, RA Seabourn, B Chen, YHR Blechl, AE AF Graybosch, Robert A. Seabourn, Bradford Chen, Yuanhong R. Blechl, Ann E. TI Transgenic Enhancement of High-Molecular-Weight Glutenin Subunit 1Dy10 Concentration: Effects in Wheat Flour Blends and Sponge and Dough Baking SO CEREAL CHEMISTRY LA English DT Article ID FUNCTIONAL-PROPERTIES; MIXING PROPERTIES; LINES; QUALITY; 1AX1; GENE; STRENGTH; PROTEIN; 1DX5 AB Dough strength is needed for efficient breadmaking quality. This property is strongly influenced in wheat (Triticum aestivum L.) by gluten seed storage proteins and, in particular, by high-molecular-weight (HMW) glutenin subunit composition. Experiments were designed to elevate expression of a key native HMW glutenin subunit (1Dy10) via genetic engineering and to determine whether resultant flours can be used in sponge and dough applications, the most common commercial bread-baking procedure. Both unblended and blended samples from transgenic and nontransgenic sister lines were tested, with blended samples being formed by addition to a control sample. Dough properties, as determined by farinograph evaluation, were improved by the transgene-encoded increases in 1Dy10 in both undiluted and blended flours. Mean farinograph stability of transgenic samples was twice that of the control, and blends with transgenic samples demonstrated increases in stabilities proportional to the amount of transgenic flour included. Mean farinograph quality numbers of transgenic samples, and of all blends containing transgenic flour, were significantly higher than both the control and all nontransgenic treatments. In the sponge and dough bake procedure, undiluted transgenic samples induced lower scores, relative to both control and undiluted nontransgenic samples, for water absorption, crumb body firmness, and loaf volume. In blends, however, the transgenic samples resulted in improvements in some sponge and dough loaf attributes, including loaf symmetry and crumb color score, without any concomitant loss of loaf volume in transgenic blends. These improved variables relate to finished product appearance and to consumer selection in markets. The use of transgenic flours with increased 1Dy10 glutenin content in commercial blends could provide advantages in sponge and dough bake applications. C1 [Graybosch, Robert A.] Univ Nebraska, ARS, USDA, Lincoln, NE 68583 USA. [Seabourn, Bradford; Chen, Yuanhong R.] ARS, USDA, Hard Winter Wheat Qual Lab, Manhattan, KS USA. [Blechl, Ann E.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. RP Graybosch, RA (reprint author), Univ Nebraska, ARS, USDA, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM bob.graybosch@ars.usda.gov NR 21 TC 2 Z9 3 U1 1 U2 15 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2013 VL 90 IS 2 BP 164 EP 168 DI 10.1094/CCHEM-09-12-0115-R PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 099TO UT WOS:000315645200012 ER PT J AU Bestelmeyer, BT Duniway, MC James, DK Burkett, LM Havstad, KM AF Bestelmeyer, Brandon T. Duniway, Michael C. James, Darren K. Burkett, Laura M. Havstad, Kris M. TI A test of critical thresholds and their indicators in a desertification-prone ecosystem: more resilience than we thought SO ECOLOGY LETTERS LA English DT Article DE Desertification; early warning; grazing; long-term experiment; patch size; regime shift; resilience; state-transition model ID PATCH SIZE DISTRIBUTION; REGIME SHIFTS; GLOBAL DESERTIFICATION; ECOLOGICAL THRESHOLDS; SUITABLE INDICATOR; TIPPING POINT; VEGETATION; MANAGEMENT; RECOVERY; RESTORATION AB Theoretical models predict that drylands can cross critical thresholds, but experimental manipulations to evaluate them are non-existent. We used a long-term (13-year) pulse-perturbation experiment featuring heavy grazing and shrub removal to determine if critical thresholds and their determinants can be demonstrated in Chihuahuan Desert grasslands. We asked if cover values or patch-size metrics could predict vegetation recovery, supporting their use as early-warning indicators. We found that season of grazing, but not the presence of competing shrubs, mediated the severity of grazing impacts on dominant grasses. Recovery occurred at the same rate irrespective of grazing history, suggesting that critical thresholds were not crossed, even at low cover levels. Grass cover, but not patch size metrics, predicted variation in recovery rates. Some transition-prone ecosystems are surprisingly resilient; management of grazing impacts and simple cover measurements can be used to avert undesired transitions and initiate restoration. C1 [Bestelmeyer, Brandon T.; Duniway, Michael C.; James, Darren K.; Burkett, Laura M.; Havstad, Kris M.] New Mexico State Univ, USDA, ARS, Jornada Expt Range & Jornada Basin LTER, Las Cruces, NM 88003 USA. [Duniway, Michael C.] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. RP Bestelmeyer, BT (reprint author), New Mexico State Univ, USDA, ARS, Jornada Expt Range & Jornada Basin LTER, Box 30003,MSC 3JER, Las Cruces, NM 88003 USA. EM bbestelm@nmsu.edu OI Duniway, Michael/0000-0002-9643-2785 NR 47 TC 34 Z9 34 U1 6 U2 177 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD MAR PY 2013 VL 16 IS 3 BP 339 EP 345 DI 10.1111/ele.12045 PG 7 WC Ecology SC Environmental Sciences & Ecology GA 088VS UT WOS:000314865900008 PM 23216915 ER PT J AU Cole, DN AF Cole, David N. TI Long-Term Effectiveness of Restoration Treatments on Closed Wilderness Campsites SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Compost; Recreation impacts; Scarification; Seeding; Soil amendments; Transplanting ID SEEDLING ESTABLISHMENT; NATIONAL-PARK; TURF TRANSPLANTS; SWISS ALPS; ALPINE; REVEGETATION; VEGETATION; COLORADO; SUCCESS; USA AB This study assessed long-term recovery of vegetation on six wilderness campsites in subalpine forests in Oregon that were closed to use and that received common restoration treatments (scarification, soil amendments, mulch, transplanting, and seeding). Vegetation cover was assessed every year for the first 7 years following treatment, as well as 10 and 15 years after treatment. This made it possible to compare long-term treatment effectiveness to short-term efficacy. Plots that were closed and not scarified had virtually no vegetation cover even after 15 years without use. If long-used campsites in these subalpine forests are simply closed and allowed to recover on their own, restoration of undisturbed conditions will require hundreds if not thousands of years. Study results show, however, that simple treatments can accelerate recovery rates substantially. Scarification and transplanting were highly effective treatments, with seeding and soil amendment with organic matter and compost also contributing to success, but to a lesser degree. The use of a mulch mat, in contrast, had no effect, either positive or negative. Assessments of success conducted within the first few years of treatment overestimate treatment efficacy, particularly the effectiveness of soil amendments and seeding. C1 US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Cole, DN (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, 790 East Beckwith, Missoula, MT 59801 USA. EM dcole@bigsky.net FU Forest Service, US Department of Agriculture FX Funding and in-kind support for this project were provided by the Forest Service, US Department of Agriculture. I appreciate the field assistance of many Forest Service personnel, particularly Dave Spildie, and the statistical advice of Dave Turner. NR 32 TC 2 Z9 2 U1 1 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X J9 ENVIRON MANAGE JI Environ. Manage. PD MAR PY 2013 VL 51 IS 3 BP 642 EP 650 DI 10.1007/s00267-012-0015-3 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA 096PO UT WOS:000315416900010 PM 23322127 ER PT J AU Mavrodi, DV Parejko, JA Mavrodi, OV Kwak, YS Weller, DM Blankenfeldt, W Thomashow, LS AF Mavrodi, Dmitri V. Parejko, James A. Mavrodi, Olga V. Kwak, Youn-Sig Weller, David M. Blankenfeldt, Wulf Thomashow, Linda S. TI Recent insights into the diversity, frequency and ecological roles of phenazines in fluorescent Pseudomonas spp. SO ENVIRONMENTAL MICROBIOLOGY LA English DT Review ID ANTIBIOTIC PHENAZINE-1-CARBOXYLIC ACID; AUREOFACIENS STRAIN 30-84; BIOFILM FORMATION; BIOLOGICAL-CONTROL; POPULATION-STRUCTURE; SWARMING MOTILITY; BIOSYNTHETIC-PATHWAY; SOIL SUPPRESSIVENESS; AERUGINOSA PA14; PROTEIN PHZF AB Phenazine compounds represent a large class of bacterial metabolites that are produced by some fluorescent Pseudomonas spp. and a few other bacterial genera. Phenazines were first noted in the scientific literature over 100 years ago, but for a long time were considered to be pigments of uncertain function. Following evidence that phenazines act as virulence factors in the opportunistic human and animal pathogen Pseudomonas aeruginosa and are actively involved in the suppression of plant pathogens, interest in these compounds has broadened to include investigations of their genetics, biosynthesis, activity as electron shuttles, and contribution to the ecology and physiology of the cells that produce them. This minireview highlights some recent and exciting insights into the diversity, frequency and ecological roles of phenazines produced by fluorescent Pseudomonas spp. C1 [Mavrodi, Dmitri V.; Mavrodi, Olga V.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Parejko, James A.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Kwak, Youn-Sig] Gyeongsang Natl Univ, Dept Appl Biol, Jinju, Gyeongnam, South Korea. [Weller, David M.; Thomashow, Linda S.] ARS, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Blankenfeldt, Wulf] Univ Bayreuth, Dept Biochem, D-95447 Bayreuth, Germany. RP Mavrodi, DV (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM dmavrodi@wsu.edu RI Mavrodi, Dmitri/J-2745-2013; Blankenfeldt, Wulf/F-8877-2010 OI Blankenfeldt, Wulf/0000-0001-9886-9668 FU USDA-NIFA Soil Processes Program [2011-67019-30212] FX The research was supported in part by Grant 2011-67019-30212 from the USDA-NIFA Soil Processes Program. NR 96 TC 26 Z9 28 U1 5 U2 97 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD MAR PY 2013 VL 15 IS 3 SI SI BP 675 EP 686 DI 10.1111/j.1462-2920.2012.02846.x PG 12 WC Microbiology SC Microbiology GA 100SR UT WOS:000315723300001 PM 22882648 ER PT J AU Kidarsa, TA Shaffer, BT Goebel, NC Roberts, DP Buyer, JS Johnson, A Kobayashi, DY Zabriskie, TM Paulsen, I Loper, JE AF Kidarsa, Teresa A. Shaffer, Brenda T. Goebel, Neal C. Roberts, Daniel P. Buyer, Jeffrey S. Johnson, Aaron Kobayashi, Donald Y. Zabriskie, T. Mark Paulsen, Ian Loper, Joyce E. TI Genes expressed by the biological control bacterium Pseudomonas protegens Pf-5 on seed surfaces under the control of the global regulators GacA and RpoS SO ENVIRONMENTAL MICROBIOLOGY LA English DT Review ID COMPLETE GENOME SEQUENCE; FLUORESCENS BIOCONTROL STRAINS; AERUGINOSA BIOFILM DEVELOPMENT; TONB-DEPENDENT TRANSPORTERS; SIGNAL-TRANSDUCTION PATHWAY; SIGMA-FACTOR SIGMA(S); PYTHIUM DAMPING-OFF; ANTIBIOTIC PRODUCTION; SMALL RNAS; ESCHERICHIA-COLI AB Gene expression profiles of the biological control strain Pseudomonas protegens Pf-5 inhabiting pea seed surfaces were revealed using a whole-genome oligonucleotide microarray. We identified genes expressed by Pf-5 under the control of two global regulators (GacA and RpoS) known to influence biological control and secondary metabolism. Transcript levels of 897 genes, including many with unknown functions as well as those for biofilm formation, cyclic diguanylate (c-di-GMP) signalling, iron homeostasis and secondary metabolism, were influenced by one or both regulators, providing evidence for expression of these genes by Pf-5 on seed surfaces. Comparison of the GacA and RpoS transcriptomes defined for Pf-5 grown on seed versus in broth culture overlapped, but most genes were regulated by GacA or RpoS under only one condition, likely due to differing levels of expression in the two conditions. We quantified secondary metabolites produced by Pf-5 and gacA and rpoS mutants on seed and in culture, and found that production profiles corresponded generally with biosynthetic gene expression profiles. Future studies evaluating biological control mechanisms can now focus on genes expressed by Pf-5 on seed surfaces, the habitat where the bacterium interacts with seed-infecting pathogens to suppress seedling diseases. C1 [Kidarsa, Teresa A.; Shaffer, Brenda T.; Loper, Joyce E.] USDA ARS Hort Crops Res Lab, Corvallis, OR USA. [Goebel, Neal C.; Zabriskie, T. Mark] Oregon State Univ, Dept Pharmaceut Sci, Corvallis, OR 97331 USA. [Roberts, Daniel P.; Buyer, Jeffrey S.] USDA ARS Sustainable Agr Syst Lab, Beltsville, MD USA. [Johnson, Aaron] J Craig Venter Inst, Rockville, MD USA. [Kobayashi, Donald Y.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. [Paulsen, Ian] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. RP Loper, JE (reprint author), USDA ARS Hort Crops Res Lab, Corvallis, OR USA. EM Joyce.Loper@ars.usda.gov RI Paulsen, Ian/K-3832-2012; OI Buyer, Jeffrey/0000-0003-2098-0547; Paulsen, Ian/0000-0001-9015-9418; Loper, Joyce/0000-0003-3501-5969 FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2006-35319-17427] FX We are grateful to Caroline Press for developing methods for RNA isolation from Pf-5 growing on seed surfaces, Marcella Henkels for technical assistance, and Ed Davis for assistance with identification of the Anr binding sites. This project was supported by National Research Initiative Competitive Grant 2006-35319-17427 from the USDA National Institute of Food and Agriculture. NR 126 TC 13 Z9 14 U1 2 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD MAR PY 2013 VL 15 IS 3 SI SI BP 716 EP 735 DI 10.1111/1462-2920.12066 PG 20 WC Microbiology SC Microbiology GA 100SR UT WOS:000315723300004 PM 23297839 ER PT J AU Hunt, CG Houtman, CJ Jones, DC Kitin, P Korripally, P Hammel, KE AF Hunt, Christopher G. Houtman, Carl J. Jones, Don C. Kitin, Peter Korripally, Premsagar Hammel, Kenneth E. TI Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FUNGUS PHANEROCHAETE-CARNOSA; ALCOHOL RADICAL-CATION; VERATRYL ALCOHOL; MANGANESE PEROXIDASE; CELLOBIOSE DEHYDROGENASE; SECRETOME ANALYSIS; DEGRADING ENZYME; CHRYSOSPORIUM; BIODEGRADATION; DEGRADATION AB Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and is accomplished most efficiently by fungi that cause white rot of wood. These basidiomycetes secrete many enzymes and metabolites with proposed ligninolytic roles, and it is not clear whether all of these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem is to infer properties of ligninolytic oxidants from their spatial distribution relative to the fungus on the lignocellulose. We grew Phanerochaete chrysosporium on wood sections in the presence of oxidant-sensing beads based on the ratiometric fluorescent dye BODIPY 581/591. The beads, having fixed locations relative to the fungal hyphae, enabled spatial mapping of cumulative extracellular oxidant distributions by confocal fluorescence microscopy. The results showed that oxidation gradients occurred around the hyphae, and data analysis using a mathematical reactiondiffusion model indicated that the dominant oxidant during incipient white rot had a half-life under 0.1s. The best available hypothesis is that this oxidant is the cation radical of the secreted P.chrysosporium metabolite veratryl alcohol. C1 [Hunt, Christopher G.; Houtman, Carl J.; Jones, Don C.; Kitin, Peter; Korripally, Premsagar; Hammel, Kenneth E.] US Forest Serv, Madison, WI 53726 USA. [Kitin, Peter; Korripally, Premsagar; Hammel, Kenneth E.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Hunt, CG (reprint author), US Forest Serv, Madison, WI 53726 USA. EM cghunt@fs.fed.us RI Hammel, Kenneth/G-1890-2011; Kitin, Peter/D-9244-2014 OI Hammel, Kenneth/0000-0002-2935-5847; FU U.S. Department of Energy, Office of Biological and Environmental Research [DE-AI02-07ER64491, DE-SC0006929] FX We thank Vitaliy Timokhin for syntheses of BODIPY beads. This work was supported by grants DE-AI02-07ER64491 (C. G. H. and K. E. H.) and DE-SC0006929 (K. E. H., C. G. H., and C. J. H.) from the U.S. Department of Energy, Office of Biological and Environmental Research. Confocal imaging was performed at the Plant Imaging Center, Department of Botany, University of Wisconsin-Madison. The authors declare no conflicts of interest. NR 50 TC 9 Z9 9 U1 5 U2 76 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD MAR PY 2013 VL 15 IS 3 SI SI BP 956 EP 966 DI 10.1111/1462-2920.12039 PG 11 WC Microbiology SC Microbiology GA 100SR UT WOS:000315723300021 PM 23206186 ER PT J AU Bradford, DF Stanley, KA Tallent, NG Sparling, DW Nash, MS Knapp, RA McConnell, LL Simonich, SLM AF Bradford, David F. Stanley, Kerri A. Tallent, Nita G. Sparling, Donald W. Nash, Maliha S. Knapp, Roland A. McConnell, Laura L. Simonich, Staci L. Massey TI Temporal and spatial variation of atmospherically deposited organic contaminants at high elevation in yosemite national park, California, USA SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Cholinesterase; Pseudacris sierra; Sediment; Sierra Nevada; Tadpole ID SIERRA-NEVADA MOUNTAINS; CURRENT-USE PESTICIDES; AMPHIBIAN POPULATION DECLINES; YELLOW-LEGGED FROGS; ALPINE LAKES; ACCUMULATION; PATTERNS; FISH; GUIDELINES; TADPOLES AB Contaminants used at low elevation, such as pesticides on crops, can be transported tens of kilometers and deposited in adjacent mountains in many parts of the world. Atmospherically deposited organic contaminants in the Sierra Nevada Mountains of California, USA, have exceeded some thresholds of concern, but the spatial and temporal distributions of contaminants in the mountains are not well known. The authors sampled shallow-water sediment and tadpoles (Pseudacris sierra) for pesticides, polycyclic aromatic hydrocarbons (PAHs), and polychlorinated biphenyls in four high-elevation sites in Yosemite National Park in the central Sierra Nevada twice during the summers of 2006, 2007, and 2008. Both historic- and current-use pesticides showed a striking pattern of lower concentrations in both sediment and tadpoles in Yosemite than was observed previously in SequoiaKings Canyon National Parks in the southern Sierra Nevada. By contrast, PAH concentrations in sediment were generally greater in Yosemite than in SequoiaKings Canyon. The authors suggest that pesticide concentrations tend to be greater in SequoiaKings Canyon because of a longer air flow path over agricultural lands for this park along with greater pesticide use near this park. Concentrations for DDT-related compounds in some sediment samples exceeded guidelines or critical thresholds in both parks. A general pattern of difference between Yosemite and SequoiaKings Canyon was not evident for total tadpole cholinesterase activity, an indicator of harmful exposure to organophosphorus and carbamate pesticides. Variability of chemical concentrations among sites, between sampling periods within each year, and among years, contributed significantly to total variation, although the relative contributions differed between sediment and tadpoles. Environ. Toxicol. Chem. 2013;32:517525. (c) 2012 SETAC C1 [Bradford, David F.; Tallent, Nita G.; Nash, Maliha S.] US EPA, Natl Exposure Res Lab, Las Vegas, NV 89193 USA. [Stanley, Kerri A.; Simonich, Staci L. Massey] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA. [Sparling, Donald W.] So Illinois Univ, Cooperat Wildlife Res Lab, Carbondale, IL 62901 USA. [Knapp, Roland A.] Univ Calif, Sierra Nevada Aquat Res Lab, Mammoth Lakes, CA USA. [McConnell, Laura L.] ARS, USDA, Beltsville, MD USA. [Simonich, Staci L. Massey] Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA. RP Bradford, DF (reprint author), US EPA, Natl Exposure Res Lab, Las Vegas, NV 89193 USA. EM bradford.david@epa.gov FU U.S. Environmental Protection Agency (U.S. EPA) [DW1492238701]; National Park Service; U.S. Department of Agriculture; [DW1292244701] FX We are grateful to J. Maurer for help with field sampling, to S. Thompson, A. Williams, and A. Esperanza for help in various ways, and to D. Chaloud for reviewing a previous version of the manuscript. We thank the National Park Service for their cooperation on the project and managing the cooperative agreements to support the chemical analyses. The research described herein was partially funded by the U.S. Environmental Protection Agency (U.S. EPA) through interagency agreements DW1492238701 with the National Park Service and DW1292244701 with the U.S. Department of Agriculture. The article has been approved for publication by the U.S. EPA. NR 32 TC 8 Z9 8 U1 3 U2 69 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2013 VL 32 IS 3 BP 517 EP 525 DI 10.1002/etc.2094 PG 9 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 092KG UT WOS:000315119000006 PM 23233353 ER PT J AU King, KW Balogh, JC AF King, Kevin W. Balogh, James C. TI Event based analysis of chlorothalonil concentrations following application to managed turf SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Pesticides; Water quality; Golf; Watershed; Runoff ID TRANSPORT; WATER AB Chlorothalonil concentrations exceeding acute toxicity levels for certain organisms have been measured in surface water discharge events from managed turf watersheds. The duration of exceedence and the timing of these events related to precipitation/runoff and time since application, however, have not been explored. Chlorothalonil concentrations were measured from discharge waters draining a managed turf watershed in Duluth, Minnesota, USA, between 2003 and 2009. The median chlorothalonil concentration was 0.58 mu g/L. Approximately 2% of all measured concentrations exceeded the 7.6 mu g/L median lethal concentration (LC50) acute toxicity level for rainbow trout. One-twentieth the LC50 concentration, equivalent to the level of concern (0.38 mu g/L) for endangered species, was exceeded 31% of the time during the present study. The concentrations that exceeded the LC50 threshold were associated with eight rainfall/runoff events. Low dose exposures are a more important biological concern than acute occurrences. Exceedence concentrations associated with acute effects were significantly (p<0.05) correlated to time since application and were measured only in the fall following extensive application. A conflict exists between the transportability of chlorothalonil as suggested by its chemical properties and the data collected in the present study. With respect to course-wide golf course application, avoiding application until after the major autumn rainfall period but before the first snow coverage is recommended to reduce occurrence of chlorothalonil concentrations that exceed toxic levels associated with acute and chronic levels of concern. Environ. Toxicol. Chem. 2013;32:684691. (c) 2012 SETAC C1 [King, Kevin W.] ARS, USDA, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Balogh, James C.] Spectrum Res, Duluth, MN USA. RP King, KW (reprint author), ARS, USDA, Soil Drainage Res Unit, Columbus, OH 43210 USA. EM kevin.king@ars.usda.gov FU U.S. Golf Association Green Section FX The authors acknowledge the assistance of S. B. Scadlock, I. Leland, E. Burgess, H. McMains, and S. Hess in the data collection, processing, and analysis necessary to complete this study. Without their dedication, perseverance, and attention to detail, the present study would not have been possible. The authors are also indebted to D. Kolbry (past superintendent), C. Tritabaugh (current superintendent), and J. Ryan (current assistant superintendent) for granting us access to the course and sharing their management strategies and records. We also thank the members of NCC for their insight in recognizing the importance of this type of research and their willingness to permit us to conduct this study on their course. We also acknowledge and thank the U.S. Golf Association Green Section for its support and financial contributions. NR 25 TC 1 Z9 1 U1 2 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD MAR PY 2013 VL 32 IS 3 BP 684 EP 691 DI 10.1002/etc.2092 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 092KG UT WOS:000315119000025 PM 23233324 ER PT J AU Clark, DJ Manini, TM Fielding, RA Patten, C AF Clark, David J. Manini, Todd M. Fielding, Roger A. Patten, Carolynn TI Neuromuscular determinants of maximum walking speed in well-functioning older adults SO EXPERIMENTAL GERONTOLOGY LA English DT Article DE Aging; Walking; Mobility; Muscle; Electromyography ID PHYSICAL PERFORMANCE; MUSCLE POWER; MOTOR UNIT; TRICEPS SURAE; GAIT SPEED; MEANINGFUL CHANGE; HEALTHY-SUBJECTS; ELDERLY-MEN; AGE; SARCOPENIA AB Maximum walking speed may offer an advantage over usual walking speed for clinical assessment of age-related declines in mobility function that are due to neuromuscular impairment. The objective of this study was to determine the extent to which maximum walking speed is affected by neuromuscular function of the lower extremities in older adults. We recruited two groups of healthy, well functioning older adults who differed primarily on maximum walking speed. We hypothesized that individuals with slower maximum walking speed would exhibit reduced lower extremity muscle size and impaired plantarflexion force production and neuromuscular activation during a rapid contraction of the triceps surae muscle group (soleus (SO) and gastrocnemius (MG)). All participants were required to have usual 10-meter walking speed of >1.0 m/s. If the difference between usual and maximum 10 m walking speed was <0.6 m/s, the individual was assigned to the "Slower" group (n=8). If the difference between usual and maximum 10-meter walking speed was >0.6 m/s, the individual was assigned to the "Faster" group (n=12). Peak rate of force development (RFD) and rate of neuromuscular activation (rate of EMG rise) of the triceps surae muscle group were assessed during a rapid plantarflexion movement. Muscle cross sectional area of the right triceps surae, quadriceps and hamstrings muscle groups was determined by magnetic resonance imaging. Across participants, the difference between usual and maximal walking speed was predominantly dictated by maximum walking speed (r=.85). We therefore report maximum walking speed (1.76 and 2.17 m/s in Slower and Faster, p<.001) rather than the difference between usual and maximal. Plantarflexion RFD was 38% lower (p=.002) in Slower compared to Faster. MG rate of EMG rise was 34% lower (p=.01) in Slower than Faster, but SO rate of EMG rise did not differ between groups (p=.73). Contrary to our hypothesis, muscle CSA was not lower in Slower than Faster for the muscle groups tested, which included triceps surae (p=.44), quadriceps (p=.76) and hamstrings (p=.98). MG rate of EMG rise was positively associated with RFD and maximum 10 m walking speed, but not the usual 10 m walking speed. These findings support the conclusion that maximum walking speed is limited by impaired neuromuscular force and activation of the triceps surae muscle group. Future research should further evaluate the utility of maximum walking speed for use in clinical assessment to detect and monitor age-related functional decline. Published by Elsevier Inc. C1 [Clark, David J.; Patten, Carolynn] Malcom Randall VA Med Ctr, Brain Rehabil Res Ctr, Gainesville, FL 32608 USA. [Clark, David J.; Manini, Todd M.] Univ Florida, Inst Aging, Gainesville, FL USA. [Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Patten, Carolynn] Univ Florida, Dept Phys Therapy, Gainesville, FL USA. RP Clark, DJ (reprint author), Malcom Randall VA Med Ctr, Brain Rehabil Res Ctr 151A, 1601 SW Archer Rd, Gainesville, FL 32608 USA. EM davidclark@ufl.edu RI Patten, Carolynn/B-4804-2009 OI Patten, Carolynn/0000-0002-9948-0045 FU University of Florida Claude D. Pepper Older Americans Independence Center [P30-AG028740-04]; U.S. Department of Veterans Affairs Rehabilitation Research and Development Service [B7176-W]; U.S. Department of Agriculture [58-1950-7-707]; Boston Claude D. Pepper Older Americans Independence Center; Boston Rehabilitation Outcomes Center FX This work was supported by the University of Florida Claude D. Pepper Older Americans Independence Center (P30-AG028740-04) and by the U.S. Department of Veterans Affairs Rehabilitation Research and Development Service (B7176-W to DJC). This work was also supported by the U.S. Department of Agriculture, under agreement no. 58-1950-7-707, the Boston Claude D. Pepper Older Americans Independence Center and the Boston Rehabilitation Outcomes Center. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Veterans Affairs or U.S. Department of Agriculture. NR 59 TC 17 Z9 17 U1 0 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0531-5565 J9 EXP GERONTOL JI Exp. Gerontol. PD MAR PY 2013 VL 48 IS 3 BP 358 EP 363 DI 10.1016/j.exger.2013.01.010 PG 6 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA 100FZ UT WOS:000315683800008 PM 23376102 ER PT J AU Cao, Y Colegate, SM Edgar, JA AF Cao, Y. Colegate, S. M. Edgar, J. A. TI Persistence of echimidine, a hepatotoxic pyrrolizidine alkaloid, from honey into mead SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Echium plantagineum; Pyrrolizidine alkaloids; Echimidine; Honey; Mead; HPLC-MS; Food safety; Food analysis; Food composition ID SOLID-PHASE EXTRACTION; ECHIUM-PLANTAGINEUM L; LC-MS ANALYSIS; N-OXIDES; POLLEN; FOOD AB Honey produced by bees foraging on Echium plantagineum is known to contain dehydropyrrolizidine alkaloids characteristic of the plant. Following a prolific growth of E. plantagineum in the wake of Australian bushfires, two samples of mead, a fermented drink made from honey, and the honey used to prepare the mead were analyzed for the presence of Echium-related dehydropyrrolizidine alkaloids. HPLC-esiMS and MS/MS analysis of the alkaloidal fractions obtained using strong cation exchange, solid phase extraction unequivocally confirmed the presence of echimidine, a major hepatotoxic dehydropyrrolizidine alkaloid produced by E. plantagineum, in the honey (780 ng/g) and in the subsequent mead samples (236-540 ng/mL). The results from this limited, and specifically targeted sample set, while not indicative of the extent of the presence of echimidine (or other dehydropyrrolizidine alkaloids) in meads, reinforce the need for a wider survey and perhaps subsequent routine monitoring to determine the potential contribution to long-term, low-level or intermittent exposure to these toxic alkaloids and consequent chronic disease development. Published by Elsevier Inc. C1 [Cao, Y.] CSIRO Livestock Ind, Geelong, Vic, Australia. [Colegate, S. M.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84321 USA. [Edgar, J. A.] CSIRO Food & Nutr Sci, N Ryde, NSW, Australia. RP Colegate, SM (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84321 USA. EM steven.colegate@ars.usda.gov NR 15 TC 10 Z9 11 U1 2 U2 42 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD MAR PY 2013 VL 29 IS 2 BP 106 EP 109 DI 10.1016/j.jfca.2012.11.005 PG 4 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 096UF UT WOS:000315429100004 ER PT J AU Exler, J Phillips, KM Patterson, KY Holden, JM AF Exler, Jacob Phillips, Katherine M. Patterson, Kristine Y. Holden, Joanne M. TI Cholesterol and vitamin D content of eggs in the US retail market SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Eggs; Vitamin D; 25-Hydroxyvitamin D; 25-Hydroxyvitamin D-3; Cholecalciferol; Cholesterol; Sterols; Lipids; Food composition; Food analysis; Food data compilation and management; Ultra-high performance liquid chromatography tandem mass spectrometry; HPLC; MS; LC-MS ID NUTRIENT ANALYSIS PROGRAM; NATIONAL FOOD; MASS-SPECTROMETRY; STRATEGIES; VALIDATION AB Nationwide sampling in the U.S. of whole large eggs, to update values in the United States Department of Agriculture (USDA) National Nutrient Database for Standard Reference (SR) (http://www.ars.usda.gov/nutrientdata), was conducted in 2000-2001 and in 2010. Retail cartons of large eggs were obtained from 12 supermarket locations using statistical sampling plans based on market share and census data. Cholesterol was analyzed at three laboratories using standard methods involving gas chromatography of the saponified total lipid extract. Vitamin D-3 and 25-OH-vitamin D-3 (2010 samples only) were analyzed by HPLC and UHPLC-MS/MS. Quality control materials were included to validate the accuracy and precision of measurements. The mean cholesterol content decreased 51 mg/100 g (12%; p < 0.0001), from 423 mg/100 g in 2000-2001 to 372 (range 344-405) in 2010. Over the same period, average vitamin D-3 increased by 60%, to 2.05 mu g [80 IU]/100 g (range 0.97-12.1). Samples from 2010 contained 0.65 mu g 25-OH-D-3/100 g (range 0.43-1.32). The disparate vitamin D (and cholesterol) content of eggs sampled from different locations may reflect industry efforts to modify poultry feed or supplements to affect the nutrient profile of eggs. Cholesterol and vitamin D-3 data from this work were included in SR release 23, and support food consumption surveys, food and nutrition policy, and consumer education. (C) 2012 Elsevier Inc. All rights reserved. C1 [Exler, Jacob; Patterson, Kristine Y.; Holden, Joanne M.] ARS, USDA, Nutrient Data Lab, BARC West, Beltsville, MD 20705 USA. [Phillips, Katherine M.] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA. RP Phillips, KM (reprint author), Virginia Tech, Dept Biochem, 304 Engel Hall, Blacksburg, VA 24061 USA. EM kmpvpi@vt.edu OI Phillips, Katherine/0000-0002-4586-8538 FU Egg Nutrition Council; USDA Agricultural Research Service [Y1-HV-8116-11]; National Institutes of Health [Y1CN5010] FX The Egg Nutrition Council provided funding for the nutrient analyses at commercial laboratories. Other work was supported by the USDA Agricultural Research Service as part of the National Food and Nutrient Analysis Program, including cooperative agreement #Y1-HV-8116-11 between the USDA Nutrient Data Laboratory and Virginia Tech and from institutes of the National Institutes of Health, agreement #Y1CN5010. At Virginia Tech, the detailed work of Amy Rasor and Nancy Conley in sample preparation and of David Ruggio in cholesterol analysis is acknowledged. The vitamin D analyses by Dr. W. Craig Byrdwell of the USDA Food Composition and Methods Development Laboratory is acknowledged. We are grateful to Health Canada for providing samples of their in-house pork reference material. NR 32 TC 8 Z9 8 U1 2 U2 50 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD MAR PY 2013 VL 29 IS 2 BP 110 EP 116 DI 10.1016/j.jfca.2012.11.001 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 096UF UT WOS:000315429100005 ER PT J AU Zhang, LP Huang, FN Leonard, BR Chen, M Clark, T Zhu, YC Wangila, DS Yang, F Niu, Y AF Zhang, Liping Huang, Fangneng Leonard, B. Rogers Chen, Mao Clark, Thomas Zhu, Yu Cheng Wangila, David S. Yang, Fei Niu, Ying TI Susceptibility of Cry1Ab maize-resistant and -susceptible strains of sugarcane borer (Lepidoptera: Crambidae) to four individual Cry proteins SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Diatraea saccharalis; Bacillus thuringiensis; Cross-resistance; Resistance management; Bt maize ID BACILLUS-THURINGIENSIS TOXINS; EUROPEAN CORN-BORER; OSTRINIA-NUBILALIS LEPIDOPTERA; MOTH PLUTELLA-XYLOSTELLA; CROSS-RESISTANCE; PECTINOPHORA-GOSSYPIELLA; DELTA-ENDOTOXINS; FIELD-RESISTANCE; CRYSTAL PROTEINS; TRANSGENIC CORN AB Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F-2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) of resistance to Cry1Ab as shown in the known Cry1Ab-resistant strain, while resistance levels were relatively lower for other strains (116- to 129-fold). All the six strains were highly cross-resistant to Cry1Aa (71- to 292-fold) and Cry1Ac (30- to 248-fold), but only with a low level to Cry1F (<7-fold). Larval growth of all six strains was also inhibited on Bt-treated diet, but, except for Cry1F, the growth inhibition of the six strains was considerably less than that of the Cry1Ab-susceptible larvae. The results provide clear evidence that the observed resistance to Cry1Ab maize in the six strains is a result of resistance to the Cry1Ab protein in the plants. The low level of cross-resistance between Cry1A and Cry1F suggests that pyramiding these two types of Bt proteins into a plant could be a good strategy for managing D. saccharalis. (C) 2012 Elsevier Inc. All rights reserved. C1 [Zhang, Liping; Huang, Fangneng; Leonard, B. Rogers; Wangila, David S.; Yang, Fei; Niu, Ying] Louisiana State Univ, Dept Entomol, Ctr Agr, Baton Rouge, LA 70803 USA. [Zhang, Liping] Shanxi Acad Agr Sci, Cotton Res Inst, Yuncheng, Shanxi, Peoples R China. [Chen, Mao; Clark, Thomas] Monsanto Co, Chesterfield, MO 63017 USA. [Zhu, Yu Cheng] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. RP Huang, FN (reprint author), Louisiana State Univ, Dept Entomol, Ctr Agr, Baton Rouge, LA 70803 USA. EM fhuang@agcenter.lsu.edu RI Yang, Fei/Q-6498-2016 FU Louisiana Soybean and Feed Grain Promotion Board; USDA-AFRI South Regional IPM program; Monsanto Company; USDA [NC-205] FX This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript No. 2012-234-7574. This project represents work supported by the Louisiana Soybean and Feed Grain Promotion Board, USDA-AFRI South Regional IPM program, Monsanto Company, and USDA NC-205. NR 41 TC 11 Z9 11 U1 0 U2 58 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2013 VL 112 IS 3 BP 267 EP 272 DI 10.1016/j.jip.2012.12.007 PG 6 WC Zoology SC Zoology GA 097MN UT WOS:000315478300010 PM 23270874 ER PT J AU Dainat, B Neumann, P AF Dainat, Benjamin Neumann, Peter TI Clinical signs of deformed wing virus infection are predictive markers for honey bee colony losses SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Colony losses; Deformed wing virus; Predictive marker; Varroa destructor ID APIS-MELLIFERA; WINTER AB The ectoparasitic mite Varroa destructor acting as a virus vector constitutes a central mechanism for losses of managed honey bee, Apis mellifera, colonies. This creates demand for an easy, accurate and cheap diagnostic tool to estimate the impact of viruliferous mites in the field. Here we evaluated whether the clinical signs of the ubiquitous and mite-transmitted deformed wing virus (DWV) can be predictive markers of winter losses. In fall and winter 2007/2008, Am. carnica workers with apparent wing deformities were counted daily in traps installed on 29 queenright colonies. The data show that colonies which later died had a significantly higher proportion of workers with wing deformities than did those which survived. There was a significant positive correlation between V. destructor infestation levels and the number of workers displaying DWV clinical signs, further supporting the mite's impact on virus infections at the colony level. A logistic regression model suggests that colony size, the number of workers with wing deformities and V. destructor infestation levels constitute predictive markers for winter colony losses in this order of importance and ease of evaluation. (C) 2012 Elsevier Inc. All rights reserved. C1 [Dainat, Benjamin; Neumann, Peter] Agroscope Liebefeld Posieux Res Stn ALP, Swiss Bee Res Ctr, Bern, Switzerland. [Dainat, Benjamin] ARS, USDA, Bee Res Lab, Beltsville, MD USA. RP Dainat, B (reprint author), Agroscope Liebefeld Posieux ALP Haras, Swiss Bee Res Ctr, Fed Dept Econ Affairs Educ & Res EAER, Bern, Switzerland. EM benjamin.dainat@gmail.com FU Swiss Veterinary Office; USDA-ARS Bee Research Laboratory in Beltsville MD FX Financial support was granted by the Swiss Veterinary Office to BD and PN and partially by the USDA-ARS Bee Research Laboratory in Beltsville MD to BD. We are grateful to Vincent Dietemann, Werner Luginbuhl and Jay Evans for constructive comments on earlier drafts of the manuscript. NR 15 TC 14 Z9 15 U1 19 U2 98 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2013 VL 112 IS 3 BP 278 EP 280 DI 10.1016/j.jip.2012.12.009 PG 3 WC Zoology SC Zoology GA 097MN UT WOS:000315478300012 PM 23270875 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. [Gipson, Chester] USDA, APHIS, AC, Washington, DC USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 J9 LAB ANIMAL JI Lab Anim. PD MAR PY 2013 VL 42 IS 3 BP 83 EP 83 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA 096UK UT WOS:000315429900016 PM 23423295 ER PT J AU Chambers, A Carle, S Njuguna, W Chamala, S Bassil, N Whitaker, VM Barbazuk, WB Folta, KM AF Chambers, Alan Carle, Scott Njuguna, Wambui Chamala, Srikar Bassil, Nahla Whitaker, Vance M. Barbazuk, W. Brad Folta, Kevin M. TI A genome-enabled, high-throughput, and multiplexed fingerprinting platform for strawberry (Fragaria L.) SO MOLECULAR BREEDING LA English DT Article DE Strawberry; Fragaria; Simple sequence repeat; Genotyping; Genetic diversity ID SEQUENCE REPEAT MARKERS; DNA RAPD MARKERS; X ANANASSA; CULTIVATED STRAWBERRY; GENETIC DIVERSITY; POLYMORPHIC MICROSATELLITES; DIPLOID STRAWBERRY; MOLECULAR MARKERS; HIGH-RESOLUTION; F VIRGINIANA AB Strawberry (Fragaria L.) genotypes bear remarkable phenotypic similarity, even across ploidy levels. Additionally, breeding programs seek to introgress alleles from wild germplasm, so objective molecular description of genetic variation has great value. In this report, a high-throughput, robust protocol for generating highly-informative simple sequence repeat (SSR) patterns is presented to address these issues. The methods are comparable to SSR use in DNA typing in humans and are based on identification of high-number repeats composed of tetra- through nona-nucleotide repeat units found in the Fragaria vesca genome sequence. Individual SSR-containing regions were examined for variability over a range of 219 strawberry genotypes. A single-fluorophore secondary labeling strategy was devised that allows simultaneous amplification of eight SSR regions in a single PCR reaction. The approach yields reproducible, highly-variable, complex patterns (Shannon-Weaver Index 7.09-13.88). The technique may be applied to detect closely-related individuals across ploidy levels, including full sibling progeny in an inter-related octoploid pedigree. Genetic diversity among various cultivars and progenitor wild species in the United States Department of Agriculture-Agricultural Research Service Fragaria Supercore collection was also evaluated. The results build on known relationships, and also raise questions about accepted relationships between several genotypes. C1 [Chambers, Alan; Carle, Scott; Folta, Kevin M.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Njuguna, Wambui] Eurofins Lancaster Labs, Lancaster, PA 17605 USA. [Chamala, Srikar; Barbazuk, W. Brad] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Bassil, Nahla] ARS, USDA, Corvallis, OR 97333 USA. [Whitaker, Vance M.] Univ Florida, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Folta, Kevin M.] Univ Florida, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA. RP Folta, KM (reprint author), Univ Florida, Dept Hort Sci, 1301 Fifield Hall, Gainesville, FL 32611 USA. EM kfolta@ufl.edu OI Chamala, Srikar/0000-0001-6367-7615 FU University of Florida Plant Molecular Breeding Initiative FX This work was funded by the University of Florida Plant Molecular Breeding Initiative (VMW & KMF). NR 44 TC 5 Z9 5 U1 6 U2 70 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2013 VL 31 IS 3 BP 615 EP 629 DI 10.1007/s11032-012-9819-3 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 098AV UT WOS:000315516400010 ER PT J AU Xu, PZ Yan, WG He, J Li, Y Zhang, HY Peng, H Wu, XJ AF Xu, Peizhou Yan, Wengui He, Jing Li, Yun Zhang, Hongyu Peng, Hai Wu, Xianjun TI DNA methylation affected by male sterile cytoplasm in rice (Oryza sativa L.) SO MOLECULAR BREEDING LA English DT Article DE Cytoplasmic male sterility; MSAP; DNA methylation; Hybrid rice ID CYTOSINE METHYLATION; GENE-EXPRESSION; HYBRID RICE; POLYMORPHISM; AMPLIFICATION; PATTERNS; AFLP; COORDINATION; ARABIDOPSIS; NUCLEAR AB Male sterile cytoplasm plays an important role in hybrid rice, and cytoplasmic effects are sufficiently documented. However, no reports are available on DNA methylation affected by male sterile cytoplasm in hybrid rice. We used a methylation-sensitive amplified polymorphism technique to characterize DNA methylation in four male sterile cytoplasms that are widely commercialized in China. In total, 12 pairs of selective primers in combinations of EcoRI and MspI/HpaII amplified 350 bands among four male sterile (A) lines and the corresponding maintainer (B) lines. Sites b1 and b3 were fully methylated only in all the B lines, while b2 was fully methylated only in all the A lines. These results implied a relationship of DNA methylation at these sites specifically with male sterile cytoplasms, as well as male sterility, since the only difference between the A and B lines was the cytoplasm. The DNA methylation was markedly affected by male sterile cytoplasms. WA-type and Yinshui-type cytoplasms affected the methylation to a much greater degree than G-type and D-type cytoplasms, as indicated by the number and degree of methylated sites, ratio of methylated sites, number of fully methylated sites, ratio of fully methylated sites, and polymorphism between A and B lines for these cytoplasms. The genetic distance between the cytoplasm and nucleus for the WA-type is much greater than for G- and D-types because the former is between wild and cultivated species and the latter is within indica subspecies between African and Asian cultivars. This difference in genetic distance may be responsible for the variation in methylation which we observed. C1 [Xu, Peizhou; He, Jing; Zhang, Hongyu; Peng, Hai; Wu, Xianjun] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China. [Xu, Peizhou; Yan, Wengui] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Li, Yun] Chengdu Acad Agr & Forestry Sci, Chengdu 611130, Peoples R China. RP Wu, XJ (reprint author), Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China. EM yanwengui@gmail.com; wuxj@sicau.edu.cn FU National Natural Science Foundation of China [31100879] FX The authors thank Anna M. McClung and Ellen McWhirter for critical review. This work was financially supported by the National Natural Science Foundation of China (31100879). NR 45 TC 2 Z9 3 U1 1 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2013 VL 31 IS 3 BP 719 EP 727 DI 10.1007/s11032-012-9829-1 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 098AV UT WOS:000315516400018 ER PT J AU Abrams, SA Coss-Bu, JA Tiosano, D AF Abrams, Steven A. Coss-Bu, Jorge A. Tiosano, Dov TI Vitamin D: effects on childhood health and disease SO NATURE REVIEWS ENDOCRINOLOGY LA English DT Review ID INFLAMMATORY-BOWEL-DISEASE; ACUTE LYMPHOBLASTIC-LEUKEMIA; BONE-MINERAL DENSITY; RANDOMIZED CLINICAL-TRIAL; CRITICALLY-ILL CHILDREN; CHRONIC KIDNEY-DISEASE; D-RESISTANT RICKETS; D DEFICIENCY; D SUPPLEMENTATION; D-RECEPTOR AB Vitamin D is a key nutrient for both healthy children and those with chronic illnesses. Understanding its roles in health and disease has become one of the most important issues in the nutritional management of children. Formal guidelines related to nutrient requirements for vitamin D in healthy children, recommending dietary intakes of 400IU per day for infants and 600IU per day for children over 1 year of age, were released by the Institute of Medicine in November 2010. However, application of these guidelines to children with acute and chronic illnesses is less clear. In this Review, we consider major illness categories and specific examples of conditions in children that might be affected by vitamin D. This information can be used in developing both model systems of investigation and clinical trials of vitamin D in children with acute and chronic illnesses. Abrams, S. A. at al. Nat. Rev. Endocrinol. 9, 162-170 (2013); published online 5 February 2013; doi:10.1038/nrendo.2012.259 C1 [Abrams, Steven A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Coss-Bu, Jorge A.] Baylor Coll Med, Sect Crit Care, Houston, TX 77030 USA. Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Tiosano, Dov] Technion Israel Inst Technol, Bruce Rappaport Fac Med, IL-31096 Haifa, Israel. RP Abrams, SA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1 Baylor Plaza, Houston, TX 77030 USA. EM sabrams@bcm.edu OI Abrams, Steven/0000-0003-4972-9233 FU US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine; Texas Children's Hospital, Houston, TX, USA FX This work is supported by the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Texas Children's Hospital, Houston, TX, USA. Contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the us government. NR 93 TC 18 Z9 21 U1 3 U2 30 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1759-5029 J9 NAT REV ENDOCRINOL JI Nat. Rev. Endocrinol. PD MAR PY 2013 VL 9 IS 3 BP 162 EP 170 DI 10.1038/nrendo.2012.259 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 098OG UT WOS:000315558000008 PM 23381033 ER PT J AU Harwood, JF Chen, KH Muller, HG Wang, JL Vargas, RI Carey, JR AF Harwood, James F. Chen, Kehui Mueller, Hans-Georg Wang, Jane-Ling Vargas, Roger I. Carey, James R. TI Effects of diet and host access on fecundity andlifespan in two fruit fly species with different life-history patterns SO PHYSIOLOGICAL ENTOMOLOGY LA English DT Article DE Cost of reproduction; delayed reproduction; dietary restriction; medfly; melon fly; resource allocation; senescence; Tephritidae ID CERATITIS-CAPITATA; EGG-PRODUCTION; DROSOPHILA-MELANOGASTER; FLIES DIPTERA; DEMOGRAPHIC PARAMETERS; OOSTATIC HORMONE; TEPHRITIDAE; RESTRICTION; MATURATION; LONGEVITY AB The reproductive ability of female tephritids can be limited and prevented by denying access to host plants and restricting the dietary precursors of vitellogenesis. The mechanisms underlying the delayed egg production in each case are initiated by different physiological processes that are anticipated to have dissimilar effects on lifespan and reproductive ability later in life. The egg-laying abilities of laboratory-reared females of the Mediterranean fruit fly (Ceratitis capitata Wiedmann) and melon fly (Bactrocera cucurbitae Coquillett) from Hawaii are delayed or suppressed by limiting access to host fruits and dietary protein. In each case, this is expected to prevent the loss of lifespan associated with reproduction until protein or hosts are introduced. Two trends are observed in each species: first, access to protein at eclosion leads to a greater probability of survival and a higher reproductive ability than if it is delayed and, second, delayed host access reduces lifetime reproductive ability without improving life expectancy. When host access and protein availability are delayed, the rate of reproductive senescence is reduced in the medfly, whereas the rate of reproductive senescence is generally increased in the melon fly. Overall, delaying reproduction lowers the fitness of females by constraining their fecundity for the remainder of the lifespan without extending the lifespan. (c) 2013 The Royal Entomological Society C1 [Harwood, James F.; Carey, James R.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Chen, Kehui] Univ Pittsburgh, Dept Stat, Pittsburgh, PA USA. [Chen, Kehui] Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA USA. [Mueller, Hans-Georg; Wang, Jane-Ling] Univ Calif Davis, Dept Stat, Davis, CA 95616 USA. [Vargas, Roger I.] USDA ARS, PBARC, Hilo, HI USA. RP Harwood, JF (reprint author), Univ Calif Davis, Dept Entomol, 1 Shields Ave, Davis, CA 95616 USA. EM jharwood2686@gmail.com FU NIH/NIA [PO1 AG022500-01, PO1 AG08761-10] FX We thank S. Souder, R. Ijima, Y. Nakane and A. Morice for logistical support and their assistance in conducting the experiments, as well as P. Lower and F. Zalom for their comments on earlier drafts and editorial assistance. This research was funded through the NIH/NIA PO1 programme grant PO1 AG022500-01 and PO1 AG08761-10. NR 39 TC 6 Z9 7 U1 1 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6962 J9 PHYSIOL ENTOMOL JI Physiol. Entomol. PD MAR PY 2013 VL 38 IS 1 BP 81 EP 88 DI 10.1111/phen.12006 PG 8 WC Entomology SC Entomology GA 097RZ UT WOS:000315492500010 PM 23483775 ER PT J AU Fry, WE McGrath, MT Seaman, A Zitter, TA McLeod, A Danies, G Small, IM Myers, K Everts, K Gevens, AJ Gugino, BK Johnson, SB Judelson, H Ristaino, J Roberts, R Secor, G Seebold, K Snover-Clift, K Wyenandt, A Grunwald, NJ Smart, CD AF Fry, W. E. McGrath, M. T. Seaman, A. Zitter, T. A. McLeod, A. Danies, G. Small, I. M. Myers, K. Everts, K. Gevens, A. J. Gugino, B. K. Johnson, S. B. Judelson, H. Ristaino, J. Roberts, R. Secor, G. Seebold, K., Jr. Snover-Clift, K. Wyenandt, A. Gruenwald, N. J. Smart, C. D. TI The 2009 Late Blight Pandemic in the Eastern United States - Causes and Results SO PLANT DISEASE LA English DT Article ID POTATO LATE BLIGHT; INFECTED SEED TUBERS; TOMATO LATE BLIGHT; PETUNIA X HYBRIDA; PHYTOPHTHORA-INFESTANS; LATEBLIGHT MODEL; HOST-RESISTANCE; SIMULATION; GENOTYPES; SURVIVAL C1 [Fry, W. E.; McGrath, M. T.; Zitter, T. A.; Danies, G.; Small, I. M.; Myers, K.; Seebold, K., Jr.; Smart, C. D.] Cornell Univ, Ithaca, NY 14850 USA. [McLeod, A.] Univ Stellenbosch, ZA-7600 Stellenbosch, South Africa. [Everts, K.] Univ Maryland, Salisbury, England. [Gevens, A. J.] Univ Wisconsin, Madison, WI 53706 USA. [Gugino, B. K.] Penn State Univ, University Pk, PA 16802 USA. [Johnson, S. B.] Univ Maine Cooperat Extens, Presque Isle, England. [Judelson, H.] Univ Calif Riverside, Riverside, CA 92521 USA. [Ristaino, J.] N Carolina State Univ, Raleigh, NC 27695 USA. [Roberts, R.] Univ Florida, Immokalee, FL USA. [Secor, G.] N Dakota State Univ, Fargo, ND 58105 USA. [Seebold, K., Jr.] Univ Kentucky, Lexington, KY 40506 USA. [Wyenandt, A.] Rutgers Agr Res & Extens Ctr, Bridgeton, NJ USA. [Gruenwald, N. J.] USDA ARS, Hort Crops Res Unit, Washington, DC 20250 USA. RP Danies, G (reprint author), Cornell Univ, Ithaca, NY 14850 USA. EM gd243@cornell.edu RI Grunwald, Niklaus/K-6041-2013; McLeod, Adele/D-4025-2012 OI Ristaino, Jean/0000-0002-9458-0514; Grunwald, Niklaus/0000-0003-1656-7602; McLeod, Adele/0000-0002-1807-9154 FU Agriculture and Food Research Initiative Competitive Grants Program from the USDA [2011-68004-30154]; USDA NE-IPM Regional IPM program Special Grant [NYC-153560] FX This work was sponsored by the institutions of each of the authors. Additionally, some of the work was sponsored by the Agriculture and Food Research Initiative Competitive Grants Program Grant 2011-68004-30154 from the USDA, and by the USDA NE-IPM Regional IPM program Special Grant NYC-153560. NR 49 TC 35 Z9 36 U1 2 U2 75 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 296 EP 306 DI 10.1094/PDIS-08-12-0791-FE PG 11 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100001 ER PT J AU Feliziani, E Smilanick, JL Margosan, DA Mansour, MF Romanazzi, G Gu, S Gohil, HL Ames, ZR AF Feliziani, E. Smilanick, J. L. Margosan, D. A. Mansour, M. F. Romanazzi, G. Gu, S. Gohil, H. L. Ames, Z. Rubio TI Preharvest Fungicide, Potassium Sorbate, or Chitosan Use on Quality and Storage Decay of Table Grapes SO PLANT DISEASE LA English DT Article ID POSTHARVEST GRAY MOLD; BOTRYTIS-CINEREA; GREY MOLD; ANTIOXIDANT PROPERTIES; DEFENSE REACTIONS; CITRUS-FRUIT; IN-VITRO; RESVERATROL; RESISTANCE; INFECTION AB Feliziani, E., Smilanick, J. L., Margosan, D. A., Mansour, M. F., Romanazzi, G., Gu, S., Gohil, H. L., and Rubio Ames, Z. 2013. Preharvest fungicide, potassium sorbate, or chitosan use on quality and storage decay of table grapes. Plant Dis. 97:307-314. Potassium sorbate, a program of four fungicides, or one of three chitosan formulations were applied to clusters of 'Thompson Seedless' grape berries at berry set, pre-bunch closure, veraison, and 2 or 3 weeks before harvest. After storage at 2 degrees C for 6 weeks, the natural incidence of postharvest gray mold was reduced by potassium sorbate, the fungicide program, or both together in a tank mixture, in 2009 and 2010. In 2011, the experiment was repeated with three chitosan products (OII-YS, Chito Plant, and Armour-Zen) added. Chitosan or fungicide treatments significantly reduced the natural incidence of postharvest gray mold among grape berries. Berries harvested from vines treated by two of the chitosan treatments or the fungicide program had fewer infections after inoculation with Botrytis cinerea conidia. None harmed berry quality and all increased endochitinase activity. Chitosan decreased berry hydrogen peroxide content. One of the chitosan formulations increased quercetin, myricetin, and resveratrol content of the berry skin. In another experiment, 'Princess Seedless' grape treated with one of several fungicides before 4 or 6 weeks of cold storage had less decay than the control. Fenhexamid was markedly superior to the other fungicides for control of both the incidence and spread of gray mold during storage. C1 [Feliziani, E.; Romanazzi, G.] Marche Polytech Univ, Dept Agr Food & Environm Sci, I-60131 Ancona, Italy. [Smilanick, J. L.; Margosan, D. A.; Mansour, M. F.; Ames, Z. Rubio] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Gu, S.; Gohil, H. L.] Calif State Univ Fresno, Dept Viticulture & Enol, Fresno, CA 93740 USA. RP Smilanick, JL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Joe.Smilanick@ars.usda.gov RI Romanazzi, Gianfranco/C-8981-2012 FU California Table Grape Commission; BARD Project [IS-4476-11] FX We thank the California Table Grape Commission and BARD Project Number IS-4476-11 for financial support; R. Borden and L. Marais for the donation of OII-YS and Armour-Zen; and F. Mlikota Gabler, G. Verduzco, K. Fjeld, L. Cerioni, B. McCarthy, and P. Sahota for technical assistance. NR 52 TC 15 Z9 15 U1 2 U2 40 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 307 EP 314 DI 10.1094/PDIS-12-11-1043-RE PG 8 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100002 ER PT J AU Koike, ST O'Neill, N Wolf, J Van Berkum, P Daugovish, O AF Koike, Steven T. O'Neill, Nichole Wolf, Julie Van Berkum, Peter Daugovish, Oleg TI Stemphylium Leaf Spot of Parsley in California Caused by Stemphylium vesicarium SO PLANT DISEASE LA English DT Article ID PURPLE SPOT; BROWN SPOT; PEAR; ASPARAGUS; DISEASE; ONION; SEED; ETIOLOGY; BLIGHT; EPIDEMIOLOGY AB Koike, S. T., O'Neill, N., Wolf, J., Van Berkum, P., and Daugovish, O. 2013. Stemphylium leaf spot of parsley in California caused by Stemphylium vesicarium. Plant Dis. 97:315-322. From 2009 through 2011, a previously undescribed disease occurred on commercial parsley in coastal (Ventura County) California. Symptoms of the disease consisted of circular to oval, tan to brown leaf spots and resulted in loss of crop quality and, hence, reduced yields. A fungus was consistently isolated from symptomatic parsley. Morphological and molecular data identified the fungus as Stemphylium vesicarium. When inoculated onto parsley leaves, the isolates caused symptoms that were identical to those seen in the field; the same fungus was recovered from test plants, thus completing Koch's postulates. Additional inoculation experiments demonstrated that 10 of 11 tested flat leaf and curly parsley cultivars were susceptible. The parsley isolates also caused small leaf spots on other Apiaceae family plants (carrot and celery) but not on leek, onion, spinach, and tomato. Isolates caused brown lesions to form when inoculated onto pear fruit but only when the fruit tissue was wounded. Using a freeze-blotter seedborne pathogen assay, parsley seed was found to have a low incidence (0.25%) of S. vesicarium. When inoculated onto parsley leaves, three of four isolates from seed caused the same leaf spot disease. This is the first documentation of a foliar parsley disease caused by S. vesicarium. The occurrence of S. vesicarium on parsley seed indicates that infested seed may be one source of initial inoculum. Based on the negative results in the host range experiments, it appears that this parsley pathogen differs from the S. vesicarium that causes disease on leek, garlic, onion, and pear fruit. C1 [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. [O'Neill, Nichole; Wolf, Julie] USDA ARS, Beltsville, MD 20705 USA. [Van Berkum, Peter] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Daugovish, Oleg] Univ Calif Cooperat Extens, Ventura, CA 93003 USA. RP Koike, ST (reprint author), Univ Calif Cooperat Extens, Salinas, CA 93901 USA. EM stkoike@ucdavis.edu RI wolf, julie/I-6829-2012 OI wolf, julie/0000-0002-1437-982X NR 51 TC 2 Z9 2 U1 2 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 315 EP 322 DI 10.1094/PDIS-06-12-0611-RE PG 8 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100003 ER PT J AU Alderman, SC AF Alderman, Stephen C. TI Survival, Germination, and Growth of Epichloe typhina and Significance of Leaf Wounds and Insects in Infection of Orchardgrass SO PLANT DISEASE LA English DT Article ID DACTYLIS-GLOMERATA; CHOKE DISEASE; GRASSES; ENDOPHYTES; OREGON; FUNGUS; WATER; FLY AB Alderman, S. C. 2013. Survival, germination, and growth of Epichloe typhina and significance chardgrass. Plant Dis. 97:323-328. Epichloe typhina is an important stroma-producing endophytic ascomycete that is responsible for significant yield loss in orchardgrass (Dactylis glomerata) seed production fields. Infection is presumed to occur through leaves or stems, although details of the infection process and conditions that favor leaf infection are not well understood. The primary objectives of this study were to investigate the early stages of infection, including the effect of temperature or water potential on ascospore germination and subsequent growth of E. typhina, the tolerance of ascospores to desiccation, the requirement of leaf wounds for infection of orchardgrass by E. typhina, and the potential for insects to facilitate infection. Ascospores tolerated dry conditions, with at least 40% surviving 12 days under desiccation Germination and growth of of leaf wounds and insects in infection of of E. typhina was greatest at 25 degrees C, with little to no growth at 5 and 35 degrees C. Mycelial growth decreased with decreasing water potential from -0.3 to -10 MPa. Ascospore germination on leaves was predominantly hyphal at wound sites and iterative (conidiogenous) at sites without wounds. E. typhina typically entered leaves through wounds. Direct penetration was rarely observed and appeared to be associated with ascospore clusters. Germ tubes were significantly longer at sites with honeydew deposits from the bird cherry-oat aphid than at sites without honeydew. Growth of E. typhina was also observed at feeding sites of eriophyid mites, suggesting that leaf-wounding or sap-excreting insects support epiphyllous growth of E. typhina on leaves. C1 USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Alderman, SC (reprint author), USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. EM aldermas@onid.orst.edu NR 31 TC 3 Z9 5 U1 0 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 323 EP 328 DI 10.1094/PDIS-01-12-0075-RE PG 6 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100004 ER PT J AU Matos, LA Hilf, ME Cayetano, XA Feliz, AO Harper, SJ Folimonova, SY AF Matos, Luis A. Hilf, Mark E. Cayetano, Xiomara A. Feliz, Andrea O. Harper, Scott J. Folimonova, Svetlana Y. TI Dramatic Change in Citrus tristeza virus Populations in the Dominican Republic SO PLANT DISEASE LA English DT Article ID GENETIC DIVERSITY; SUBGENOMIC RNAS; SEQUENCE; CLOSTEROVIRUSES; GENOME; RECOMBINATION; EVOLUTION; INFECTION; ISOLATE; TREES AB Matos, L. A., Hill, M. E., Cayetano, X. A., Feliz, A. O., Harper, S. J., and Folimonova, S. Y. 2013. Dramatic change in Citrus tristeza virus populadons in the Dominican Republic. Plant Dis. 97:339-345. Citrus tristeza virus (CTV) is the most destructive viral pathogen of citrus and has been an important concern for the citrus industry in the Dominican Republic. Earlier studies documented widespread distribution of mild isolates of the T30 genotype, which caused no disease in the infected trees, and a low incidence of isolates of the VT and T3 genotypes, which were associated with economically damaging decline and stem-pitting symptoms in sweet orange and Persian lime, the two major citrus varieties grown in the Dominican Republic. In light of the dramatic increase in the number of severely diseased citrus trees throughout the country over the last decade, suggesting that field populations of CTV have changed, we examined the CTV pathosystem in the Dominican Republic to assess the dynamics of virus populations. In this work, we characterized the molecular composition of 163 CTV isolates from different citrus-growing regions. Our data demonstrate a dramatic change in CTV populations, with the VT genotype now widely disseminated throughout the different regions and with the presence of two new virus genotypes, T36 and RB. Multiple infections of trees resulted in development of complex virus populations composed of different genotypes. C1 [Matos, Luis A.; Harper, Scott J.; Folimonova, Svetlana Y.] Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. [Matos, Luis A.; Cayetano, Xiomara A.; Feliz, Andrea O.] IDIAF, Santo Domingo, Dominican Rep. [Hilf, Mark E.] USDA ARS, Res Serv USHRL, Ft Pierce, FL 34945 USA. [Feliz, Andrea O.] Minist Agr, Dept Sanidad Vegetal, Santo Domingo, Dominican Rep. RP Folimonova, SY (reprint author), Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. EM svetlana@ufl.edu RI Harper, Scott/O-1944-2015 OI Harper, Scott/0000-0002-9355-2431 FU Florida Agricultural Experiment Station; Ministry of Higher Education, Science, and Technology (MESCYT) in the Dominican Republic [2008-2-D1-033] FX This research was supported by the Florida Agricultural Experiment Station. We thank the Ministry of Higher Education, Science, and Technology (MESCYT) in the Dominican Republic for providing part of the support for this study under the project number 2008-2-D1-033; Consorcio Citricola del Este, Consorcio Citricos Dominicanos, and Citricos Tropicales for allowing us access to their farms for sampling; W. O. Dawson and S. M. Garnsey for insightful discussions and critical reading of the manuscript; and K. R. Sims for technical assistance in processing samples. NR 55 TC 9 Z9 11 U1 1 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 339 EP 345 DI 10.1094/PDIS-05-12-0421-RE PG 7 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100007 ER PT J AU Sharma-Poudyal, D Chen, XM Wan, AM Zhan, GM Kang, ZS Cao, SQ Jin, SL Morgounov, A Akin, B Mert, Z Shah, SJA Bux, H Ashraf, M Sharma, RC Madariaga, R Puri, KD Wellings, C Xi, KQ Wanyera, R Manninger, K Ganzalez, MI Koyda, M Sanin, S Patzek, LJ AF Sharma-Poudyal, D. Chen, X. M. Wan, A. M. Zhan, G. M. Kang, Z. S. Cao, S. Q. Jin, S. L. Morgounov, A. Akin, B. Mert, Z. Shah, S. J. A. Bux, H. Ashraf, M. Sharma, R. C. Madariaga, R. Puri, K. D. Wellings, C. Xi, K. Q. Wanyera, R. Manninger, K. Ganzalez, M. I. Koyda, M. Sanin, S. Patzek, L. J. TI Virulence Characterization of International Collections of the Wheat Stripe Rust Pathogen, Puccinia striiformis f. sp tritici SO PLANT DISEASE LA English DT Article ID UNITED-STATES; YELLOW RUST; SOMATIC RECOMBINATION; PHYSIOLOGIC RACES; EPIDEMIOLOGY; RESISTANCE; EVOLUTION; DYNAMICS; ORIGIN; CHINA AB Sharma-Poudyal, D., Chen, X. M., Wan, A. M., Zhan, G. M., Kang, Z. S., Cao, S. Q., Jin, S. L., Morgounov, A., Akin, B., Mert, Z., Shah, S. J. A., Bux, H., Ashraf, M., Sharma, R. C., Madariaga, R., Puri, K. D., Wellings, C., Xi, K. Q., Wanyera, R., Manninger, K., Ganzalez, M. I., Koyda, M., Sanin, S., and Patzek, L. J. 2013. Virulence characterization of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici. Plant Dis. 97:379-386. Wheat stripe rust (yellow rust [Yr]), caused by Puccinia striiformis f. sp. tritici, is an economically important disease of wheat worldwide. Virulence information on P striiformis f. sp. tritici populations is important to implement effective disease control with resistant cultivars. In total, 235 P striiformis f. sp. tritici isolates from Algeria, Australia, Canada, Chile, China, Hungary, Kenya, Nepal, Pakistan, Russia, Spain, Turkey, and Uzbekistan were tested on 20 single Yr-gene lines and the 20 wheat genotypes that are used to differentiate P striiformis f. sp. tritici races in the United States. The 235 isolates were identified as 129 virulence patterns on the single-gene lines and 169 virulence patterns on the U.S. differentials. Virulences to YrA,Yr2,Yr6, Yr7, Yr8, Yr9, Yr17,Yr25, YrUkn, Yr28,Yr31, YrExp2, Lemhi (Yr21), Paha (YrPa1, YrPa2, YrPa3), Druchamp (Yr3a, YrD, YrDru), Produra (YrPr1, YrPr2), Stephens (Yr3a, YrS, YrSte), Lee (Yr7, Yr22, Yr23), Fielder (Yr6, Yr20), Tyee (YrTye), Tres (YrTr1, YrTr2), Express (YrExp1,YrExp2), Clement (Yr9, YrCle), and Compair (Yr8, Yr19) were detected in all countries. At least 80% of the isolates were virulent on YrA, Yr2, Yr6,Yr7,Yr8, Yr17,YrUkn, Yr31, YrExp2,Yr21, Stephens (Yr3a, YrS, YrSte), Lee (Yr7, Yr22, Yr23), and Fielder (Yr6, Yr20). Virulences to Yr1, Yr9, Yr25, Yr27,Yr28, Heines VII (Yr2,YrHVII), Paha (YrPa1, YrPa2, YrPa3), Druchamp (Yr3a, YrD, YrDru), Produra (YrPr1, YrPr2), Yamhill (Yr2,Yr4a, YrYam), Tyee (YrTye), Tres (YrTr1, YrTr2), Hyak (Yr17,YrTye), Express (YrExp1,YrExp2), Clement (Yr9, YrCle), and Compair (Yr8,Yr19) were moderately frequent (>20 to <80%). Virulence to Yr10, Yr24, Yr32, YrSP, and Moro (Yr10, YrMor) was low (<= 20%). Virulence to Moro was absent in Algeria, Australia, Canada, Kenya, Russia, Spain, Turkey, and China, but 5% of the Chinese isolates were virulent to Yr10. None of the isolates from Algeria, Canada, China, Kenya, Russia, and Spain was virulent to Yr24; none of the isolates from Algeria, Australia, Canada, Nepal, Russia, and Spain was virulent to Yr32; none of the isolates from Australia, Canada, Chile, Hungary, Kenya, Kenya, Nepal, Pakistan, Russia, and Spain was virulent to YrSP; and none of the isolates from any country was virulent to Yr5 and Yr15. Although the frequencies of virulence factors were different, most of the P striiformis f. sp. tritici isolates from these countries shared common virulence factors. The virulences and their frequencies and distributions should be useful in breeding stripe-rust-resistant wheat cultivars and understanding the pathogen migration and evolution. C1 [Sharma-Poudyal, D.; Chen, X. M.; Wan, A. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Zhan, G. M.; Kang, Z. S.] NW A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Zhan, G. M.; Kang, Z. S.] NW A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Cao, S. Q.; Jin, S. L.] Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou, Gansu, Peoples R China. [Morgounov, A.; Akin, B.] ICARDA CIMMYT Wheat Improvement Program, Int Winter Wheat Improvement Program, Ankara, Turkey. [Mert, Z.] Cent Res Inst Field Crops, Ankara, Turkey. [Shah, S. J. A.] Nucl Inst Food & Agr, Peshawar, Pakistan. [Bux, H.] Univ Sindh Jamshoro, Inst Plant Sci, Jamshoro, Pakistan. [Ashraf, M.] Natl Univ Sci & Technol, NUST Ctr Virol & Immunol, Islamabad, Pakistan. [Sharma, R. C.] ICARDA Cent Asia & Caucasus Reg Program, Tashkent 4564, Uzbekistan. [Madariaga, R.] Natl Inst Agr Res, Chillan, Chile. [Puri, K. D.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Wellings, C.] Univ Sydney, Plant Breeding Inst, Camden, NSW, Australia. [Xi, K. Q.] Alberta Agr & Food, Field Crop Dev Ctr, Lacombe, AB, Canada. [Wanyera, R.] Kenyan Agr Res Inst, Njoro, Kenya. [Manninger, K.] Hungarian Acad Sci, Inst Plant Protect, Agr Res Ctr, Budapest, Hungary. [Ganzalez, M. I.] Limagrain Iber, Marchena, Spain. [Koyda, M.; Sanin, S.] All Russian Res Inst Phytopathol, Vyazemskii, Russia. [Patzek, L. J.] Washington State Univ, Dept Crop & Soil Sci, NWREC, Mt Vernon, IA USA. RP Chen, XM (reprint author), Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. EM xianming@wsu.edu FU United States Department of Agriculture-Agricultural Research Service [5348-22000-014-00D]; Washington State University [11W-3061-7824, 13C-3061-3925] FX This research was supported by the United States Department of Agriculture-Agricultural Research Service (project number 5348-22000-014-00D) and Washington State University (project number 11W-3061-7824 and 13C-3061-3925). We thank D. A. Johnson and T. D. Murray for critical review of the manuscript. NR 48 TC 31 Z9 32 U1 1 U2 67 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 379 EP 386 DI 10.1094/PDIS-01-12-0078-RE PG 8 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100013 ER PT J AU Grisham, MP Hoy, JW Haudenshield, JS Hartman, GL AF Grisham, M. P. Hoy, J. W. Haudenshield, J. S. Hartman, G. L. TI First Report of Orange Rust Caused by Puccinia kuehnii in Sugarcane in Louisiana SO PLANT DISEASE LA English DT News Item C1 [Grisham, M. P.] ARS, Sugarcane Res Unit, USDA, Houma, LA 70360 USA. [Hoy, J. W.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Haudenshield, J. S.; Hartman, G. L.] USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. RP Grisham, MP (reprint author), ARS, Sugarcane Res Unit, USDA, Houma, LA 70360 USA. NR 4 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2013 VL 97 IS 3 BP 426 EP 427 DI 10.1094/PDIS-09-12-0873-PDN PG 2 WC Plant Sciences SC Plant Sciences GA 095VC UT WOS:000315362100040 ER PT J AU Richardson, ML Watson, MLJ Peterson, CL AF Richardson, Matthew L. Watson, Montserrat L. J. Peterson, Cheryl L. TI Influence of community structure on the spatial distribution of critically endangered Dicerandra immaculata var. immaculata (Lamiaceae) at wild, introduced, and extirpated locations in Florida scrub SO PLANT ECOLOGY LA English DT Article DE Soil moisture; Gap specialist; Lakela's mint; Habitat ID LAKE WALES RIDGE; INTERSPECIFIC COMPETITION; BIODIVERSITY HOTSPOTS; POPULATION VIABILITY; UNITED-STATES; LAST FIRE; PLANT; HABITAT; SEED; PREFERENCES AB Community structure at local scales is a major factor controlling population and community dynamics of plant species. Dicerandra immaculata Lakela var. immaculata (Lamiaceae) is a critically endangered plant known only from a few locations in scrub habitat in Florida. Using seven sites where populations of D. immaculata were wild, introduced, and/or extirpated, we sought to answer the following questions: (1) how do habitat characteristics at locations supporting wild D. immaculata plants vary from random locations within the same habitat; (2) how do habitat characteristics differ between wild and extirpated populations; and (3) how do habitat characteristics differ between wild and introduced populations? At locations of wild D. immaculata, community structure had fewer woody stems, shorter understory vegetation, lower percent canopy coverage, and lower percent ground cover of detritus than random locations and locations with extirpated D. immaculata. In addition, bare ground decreased at extirpated locations because other plant species expanded their coverage, water saturation of the soil increased, diversity of shrubs decreased, and composition of the overstory changed compared to that of wild locations. Habitat characteristics associated with introduced plants were more similar to characteristics at randomly chosen locations than those with wild plants. However, introduced plants tended to occupy locations that had drier soil, a higher abundance of conspecifics, and a higher proportion of woody understory plants than that of random locations. Overall, gaps in the canopy and at ground level are likely essential for survival and recruitment of D. immaculata. C1 [Richardson, Matthew L.; Watson, Montserrat L. J.] ARS, Subtrop Insects & Hort Res Unit, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Peterson, Cheryl L.] Bok Tower Gardens, Rare Plant Conservat Program, Lake Wales, FL 33853 USA. RP Peterson, CL (reprint author), Bok Tower Gardens, Rare Plant Conservat Program, 1151 Tower Blvd, Lake Wales, FL 33853 USA. EM mlr1913@yahoo.com; cpeterson@boktower.org FU state of Florida, Department of Agriculture and Consumer Services, Division of Plant Industry [018698] FX M. L. Richardson and M. L. J. Watson conducted this research as unpaid volunteers and not as part of their official work duties with the USDA-ARS. The authors thank G. Bupp, J. Burns, D. Diener, C. Hew, B. Johnson, A. Loveridge, E. Mullen, K. Noland, A. Onisko, A. Richardson, J. Rynear, and L. Smith for assistance with field work. The authors also thank M. Knight and A. Griffin for help identifying study sites, public and private landowners for access to the sites, and R. Brust for expediting access to federal property and volunteer assistance. Funding was provided by a grant from the state of Florida, Department of Agriculture and Consumer Services, Division of Plant Industry (contract # 018698) to Bok Tower Gardens. NR 41 TC 4 Z9 4 U1 1 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD MAR PY 2013 VL 214 IS 3 BP 443 EP 453 DI 10.1007/s11258-013-0181-2 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 097RN UT WOS:000315491300009 ER PT J AU Georgi, L Johnson-Cicalese, J Honig, J Das, SP Rajah, VD Bhattacharya, D Bassil, N Rowland, LJ Polashock, J Vorsa, N AF Georgi, Laura Johnson-Cicalese, Jennifer Honig, Josh Das, Sushma Parankush Rajah, Veeran D. Bhattacharya, Debashish Bassil, Nahla Rowland, Lisa J. Polashock, James Vorsa, Nicholi TI The first genetic map of the American cranberry: exploration of synteny conservation and quantitative trait loci SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MICROSATELLITE MARKERS; ARABIDOPSIS-THALIANA; VACCINIUM-MACROCARPON; FUNCTIONAL-ANALYSIS; FLAVONOL SYNTHASE; CANDIDATE GENES; CANCER-CELLS; QTL ANALYSIS; LARGE SETS; FRUIT ROT AB The first genetic map of cranberry (Vaccinium macrocarpon) has been constructed, comprising 14 linkage groups totaling 879.9 cM with an estimated coverage of 82.2 %. This map, based on four mapping populations segregating for field fruit-rot resistance, contains 136 distinct loci. Mapped markers include blueberry-derived simple sequence repeat (SSR) and cranberry-derived sequence-characterized amplified region markers previously used for fingerprinting cranberry cultivars. In addition, SSR markers were developed near cranberry sequences resembling genes involved in flavonoid biosynthesis or defense against necrotrophic pathogens, or conserved orthologous set (COS) sequences. The cranberry SSRs were developed from next-generation cranberry genomic sequence assemblies; thus, the positions of these SSRs on the genomic map provide information about the genomic location of the sequence scaffold from which they were derived. The use of SSR markers near COS and other functional sequences, plus 33 SSR markers from blueberry, facilitates comparisons of this map with maps of other plant species. Regions of the cranberry map were identified that showed conservation of synteny with Vitis vinifera and Arabidopsis thaliana. Positioned on this map are quantitative trait loci (QTL) for field fruit-rot resistance (FFRR), fruit weight, titratable acidity, and sound fruit yield (SFY). The SFY QTL is adjacent to one of the fruit weight QTL and may reflect pleiotropy. Two of the FFRR QTL are in regions of conserved synteny with grape and span defense gene markers, and the third FFRR QTL spans a flavonoid biosynthetic gene. C1 [Georgi, Laura; Johnson-Cicalese, Jennifer; Vorsa, Nicholi] Rutgers State Univ, Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. [Honig, Josh; Vorsa, Nicholi] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Das, Sushma Parankush; Rajah, Veeran D.; Bhattacharya, Debashish] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [Rowland, Lisa J.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Corvallis, OR 97333 USA. [Polashock, James] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ 08019 USA. RP Vorsa, N (reprint author), Rutgers State Univ, Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. EM laura@acf.org; vorsa@aesop.rutgers.edu FU USDA SCRI [2008-51180-04878]; Ocean Spray Cranberries Incorporated FX Funding for this work was provided by USDA SCRI grant number 2008-51180-04878, with additional funding from Ocean Spray Cranberries Incorporated. We thank Kristia Adams, Dayani Stinson, and Theodore Bunch, for technical assistance, and Dylan Baker for genotyping on the AdvanCE platform. NR 69 TC 11 Z9 12 U1 1 U2 49 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2013 VL 126 IS 3 BP 673 EP 692 DI 10.1007/s00122-012-2010-8 PG 20 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 096YU UT WOS:000315441800010 PM 23224333 ER PT J AU Berger, GL Liu, SY Hall, MD Brooks, WS Chao, SM Muehlbauer, GJ Baik, BK Steffenson, B Griffey, CA AF Berger, Gregory L. Liu, Shuyu Hall, Marla D. Brooks, Wynse S. Chao, Shiaoman Muehlbauer, Gary J. Baik, B. -K. Steffenson, Brian Griffey, Carl A. TI Marker-trait associations in Virginia Tech winter barley identified using genome-wide mapping SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID POWDERY-MILDEW-RESISTANCE; GRAIN PROTEIN-CONCENTRATION; PUCCINIA-HORDEI OTTH; MALTING QUALITY; LEAF RUST; LINKAGE DISEQUILIBRIUM; BLOTCH RESISTANCE; FOOD-PRODUCTS; GENES; DISCOLORATION AB Genome-wide association studies (GWAS) provide an opportunity to examine the genetic architecture of quantitatively inherited traits in breeding populations. The objectives of this study were to use GWAS to identify chromosome regions governing traits of importance in six-rowed winter barley (Hordeum vulgare L.) germplasm and to identify single-nucleotide polymorphisms (SNPs) markers that can be implemented in a marker-assisted breeding program. Advanced hulled and hulless lines (329 total) were screened using 3,072 SNPs as a part of the US. Barley Coordinated Agricultural Project (CAP). Phenotypic data collected over 4 years for agronomic and food quality traits and resistance to leaf rust (caused by Puccinia hordei G. Otth), powdery mildew [caused by Blumeria graminis (DC.) E.O. Speer f. sp. hordei Em. Marchal], net blotch (caused by Pyrenophora teres), and spot blotch [caused by Cochliobolus sativus (Ito and Kuribayashi) Drechsler ex Dastur] were analyzed with SNP genotypic data in a GWAS to determine marker-trait associations. Significant SNPs associated with previously described quantitative trait loci (QTL) or genes were identified for heading date on chromosome 3H, test weight on 2H, yield on 7H, grain protein on 5H, polyphenol oxidase activity on 2H and resistance to leaf rust on 2H and 3H, powdery mildew on 1H, 2H and 4H, net blotch on 5H, and spot blotch on 7H. Novel QTL also were identified for agronomic, quality, and disease resistance traits. These SNP-trait associations provide the opportunity to directly select for QTL contributing to multiple traits in breeding programs. C1 [Berger, Gregory L.; Brooks, Wynse S.; Griffey, Carl A.] Virginia Tech, Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Liu, Shuyu] Texas A&M Univ, Texas AgriLife Res, Amarillo, TX 79106 USA. [Hall, Marla D.] Limagrain Cereal Seeds, Wichita, KS 67204 USA. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. [Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Baik, B. -K.] Washington State Univ, Dept Soil & Crop Sci, Pullman, WA 99164 USA. [Steffenson, Brian] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. RP Berger, GL (reprint author), Virginia Tech, Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. EM gberg06@vt.edu OI Steffenson, Brian/0000-0001-7961-5363 FU Virginia Small Grains Board; Maryland Grain Producers Utilization Board; Kentucky Small Grains Grower Association; USDA-CSREES-NRI [2006-55606-16722]; USDA-NIFA [2009-85606-05701]; [58-6645-0-108] FX This study was supported with funding provided by the Virginia Small Grains Board, the Maryland Grain Producers Utilization Board, and the Kentucky Small Grains Grower Association. This material is based on work supported by USDA-CSREES-NRI Grant no. 2006-55606-16722 and USDA-NIFA Grant no. 2009-85606-05701, "Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement", and Specific Cooperative Agreement (58-6645-0-108). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. NR 65 TC 21 Z9 22 U1 2 U2 71 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2013 VL 126 IS 3 BP 693 EP 710 DI 10.1007/s00122-012-2011-7 PG 18 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 096YU UT WOS:000315441800011 PM 23139143 ER PT J AU Schramm, EC Nelson, SK Kidwell, KK Steber, CM AF Schramm, Elizabeth C. Nelson, Sven K. Kidwell, Kimberlee K. Steber, Camille M. TI Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar 'Zak' SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; PREHARVEST SPROUTING RESISTANCE; ACID SIGNAL-TRANSDUCTION; ABSCISIC-ACID; GRAIN DORMANCY; HYPERSENSITIVE MUTANT; HEXAPLOID WHEAT; WATER-UPTAKE; ARABIDOPSIS AB As a strategy to increase the seed dormancy of soft white wheat, mutants with increased sensitivity to the plant hormone abscisic acid (ABA) were identified in mutagenized grain of soft white spring wheat "Zak". Lack of seed dormancy is correlated with increased susceptibility to preharvest sprouting in wheat, especially those cultivars with white kernels. ABA induces seed dormancy during embryo maturation and inhibits the germination of mature grain. Three mutant lines called Zak ERA8, Zak ERA19A, and Zak ERA19B (Zak ENHANCED RESPONSE to ABA) were recovered based on failure to germinate on 5 mu M ABA. All three mutants resulted in increased ABA sensitivity over a wide range of concentrations such that a phenotype can be detected at very low ABA concentrations. Wheat loses sensitivity to ABA inhibition of germination with extended periods of dry after-ripening. All three mutants recovered required more time to after-ripen sufficiently to germinate in the absence of ABA and to lose sensitivity to 5 mu M ABA. However, an increase in ABA sensitivity could be detected after as long as 3 years of after-ripening using high ABA concentrations. The Zak ERA8 line showed the strongest phenotype and segregated as a single semi-dominant mutation. This mutation resulted in no obvious decrease in yield and is a good candidate gene for breeding preharvest sprouting tolerance. C1 [Schramm, Elizabeth C.; Kidwell, Kimberlee K.; Steber, Camille M.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Schramm, Elizabeth C.; Nelson, Sven K.; Steber, Camille M.] Washington State Univ, Mol Plant Sci Program, Pullman, WA 99164 USA. [Steber, Camille M.] USDA ARS, Wheat Genet Physiol Biochem & Qual Unit, Pullman, WA 99164 USA. RP Steber, CM (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM csteber@wsu.edu FU NIH; Washington Grain Alliance; USDA CSREES [2005-01099] FX Thanks are due to S. Abrams for providing (+)-ABA, to R. Parveen and E. Getzin for expert assistance, and to K. Garland-Campbell for advice and assistance. The authors wish to thank members of the Campbell and Steber labs for helpful comments on the research and manuscript. This work was funded by an NIH protein biotechnology training grant (to ECS), by the Washington Grain Alliance (to CMS), and by USDA CSREES grant 2005-01099 (to C.M.S.). NR 52 TC 11 Z9 14 U1 0 U2 53 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2013 VL 126 IS 3 BP 791 EP 803 DI 10.1007/s00122-012-2018-0 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 096YU UT WOS:000315441800018 PM 23212773 ER PT J AU Biresaw, G Bantchev, GB AF Biresaw, Girma Bantchev, Grigor B. TI Pressure Viscosity Coefficient of Vegetable Oils SO TRIBOLOGY LETTERS LA English DT Article DE Biodegradable oils; Density; Elastohydrodynamic (EHD); Film thickness; Hexadecane; Polyalphaolefin (PAO); Pressure viscosity coefficient (PVC); Synthetic hydrocarbons; Vegetable oils; Viscosity ID FILM-FORMING PROPERTIES; BASE OIL; ELASTOHYDRODYNAMIC LUBRICATION; POLYALPHAOLEFIN BLENDS; IONIC LIQUIDS; EHD TRACTION; CASTOR-OIL; SEED OILS; TEMPERATURE; MIXTURES AB The pressure viscosity coefficient (PVC) of ten vegetable oils from commodity and new crops, and two petroleum-based oils [polyalphaolefin (PAO) and hexadecane], were investigated. PVC was estimated using two different methods: from analysis of oil physical properties (viscosity and density); and from analysis of elastohydrodynamic (EHD) film thickness data. Estimated PVC of vegetable and petroleum-based oils decreased with increasing temperature, which was expected. PVC values derived from physical properties were generally much higher than the values from the literature or those derived from EHD film thickness. PVC values increased with increasing viscosity of the tested oils. Based on further examination of PVC data for a wide range of oils from the literature and this work, it is proposed that lubricating oils can be roughly divided into three structural categories, each with defined range of PVC values: low PVC oils (< 10 GPa(-1)) with predominantly layered molecular structures; high PVC oils (> 20 GPa(-1)) with predominantly entangled molecular structures, and intermediate PVC oils (10-20 GPa(-1)) with negligible layering or entangling molecular structures. It is proposed that vegetable oils, along with PAO and hexadecane, have flexible hydrocarbon chain structures, with little or no branching or layering, and, hence, belong to the intermediate PVC category. C1 [Biresaw, Girma; Bantchev, Grigor B.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Biresaw, G (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM girma.biresaw@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 76 TC 10 Z9 10 U1 2 U2 26 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1023-8883 EI 1573-2711 J9 TRIBOL LETT JI Tribol. Lett. PD MAR PY 2013 VL 49 IS 3 BP 501 EP 512 DI 10.1007/s11249-012-0091-9 PG 12 WC Engineering, Chemical; Engineering, Mechanical SC Engineering GA 097NF UT WOS:000315480100005 ER PT J AU Wolf, KL Blahna, DJ Brinkley, W Romolini, M AF Wolf, Kathleen L. Blahna, Dale J. Brinkley, Weston Romolini, Michele TI Environmental stewardship footprint research: linking human agency and ecosystem health in the Puget Sound region SO URBAN ECOSYSTEMS LA English DT Article ID URBAN ECOLOGICAL-SYSTEMS; SOCIAL NETWORK ANALYSIS; CITIZEN SCIENCE; RESOURCE-MANAGEMENT; LONG-TERM; FRAMEWORK; VOLUNTEERS; PROGRAM; SUSTAINABILITY; MOTIVATIONS AB Urbanization processes challenge ecosystem health in many metropolitan areas. New policy and program approaches are needed to restore and sustain natural systems as public agencies and organizations face greater demands and declining budgets. Environmental stewardship is an often overlooked intervention strategy, and the full potential of civic engagement by citizens on behalf of ecosystem health is little understood. Using a coupled systems approach, integrated analysis of social and ecological footprints can lead to greater theoretical understanding and more effective programs at the landscape scale. Here we outline two pilot studies as part of an emergent research program that is investigating the extent and impact of environmental stewardship. Qualitative interviews of stewardship managers revealed multiple dimensions of motivations and purposes for stewardship, ranging from the practical to the conceptual. A regional organization census yielded a surprisingly large number of organizations that conduct stewardship, with social and ecological values being of comparable emphasis. The initial research is based in the Puget Sound area of Washington State, U.S., but results have relevance to other urban areas. Pilot study findings now guide additional research effort about motivations, organizational networks, and theory of integrated socio-ecological systems to be derived from comprehensive footprint analysis of stewardship activity. RP Wolf, KL (reprint author), USDA Forest Serv, Pacific NW Res Stn, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. EM kwolf@uw.edu NR 85 TC 12 Z9 15 U1 18 U2 114 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 J9 URBAN ECOSYST JI Urban Ecosyst. PD MAR PY 2013 VL 16 IS 1 SI SI BP 13 EP 32 DI 10.1007/s11252-011-0175-6 PG 20 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA 094XT UT WOS:000315299400003 ER PT J AU Dennis, RL Fahey, AG Cheng, HW AF Dennis, Rachel L. Fahey, Alan G. Cheng, Heng W. TI Alterations to Embryonic Serotonin Change Aggression and Fearfulness SO AGGRESSIVE BEHAVIOR LA English DT Article DE serotonin; aggression; fearfullness; avian; embryonic development ID BRAIN-DEVELOPMENT; FLUCTUATING ASYMMETRY; PRENATAL EXPOSURE; 5-HT1A RECEPTORS; NERVOUS-SYSTEM; MICE; DOPAMINE; BEHAVIOR; MOOD; CHICKEN AB Prenatal stress can alter the serotonin (5-HT) system in the developing and adult brain and lead to mood and behavioral disorders in children and adults. The chicken provides a unique animal model to study the effects of embryonic stressors on childhood and adolescent behavior. Manipulations to the egg can be made in the absence of confounding maternal effects from treatment. Eggs were injected with 50L of excess 5-HT (10g/egg), 8-OH-DPAT (a 5-HT1A receptor agonist; 16g/egg), or saline on day 0 prior to the 21 days incubation. Injections were performed at 0.5cm below the shell. Behavior was analyzed at 9 weeks of age and again at the onset of sexual maturity (18 weeks). Hens treated with excess embryonic 5-HT exhibited significantly less aggressive behaviors at 9 weeks of age compared to both 5-HT1A agonist treated and saline hens (P<0.05), and at 18 weeks of age compared to saline control birds only (P<0.05). Excess embryonic 5-HT also increased fearfulness response (P<0.05) as tested by duration of tonic immobility. Increased degree of fluctuating asymmetry at 18 weeks in 5-HT-treated birds (P<0.05) suggests that excess 5-HT in early embryonic stages may create a developmental instability, causing phenotypic variations. These results showed that modification of the serotonergic system during early embryonic development alters its functions in mediating aggressive and fearful or anxious behaviors. Prenatal modification of the serotonergic system has long lived implications on both physiology and behavior, especially aggressive and fearful behaviors. Aggr. Behav. 39:9198, 2013. Published in 2013 Wiley Periodicals, Inc. C1 [Dennis, Rachel L.; Cheng, Heng W.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Dennis, Rachel L.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Fahey, Alan G.] Univ Coll Dublin, Sch Agr Food Sci & Vet Med, Dublin 2, Ireland. RP Cheng, HW (reprint author), Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM heng-wei.cheng@ars.usda.gov OI Fahey, Alan/0000-0002-4594-5767 NR 48 TC 5 Z9 5 U1 1 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0096-140X J9 AGGRESSIVE BEHAV JI Aggressive Behav. PD MAR-APR PY 2013 VL 39 IS 2 BP 91 EP 98 DI 10.1002/ab.21459 PG 8 WC Behavioral Sciences; Psychology, Multidisciplinary SC Behavioral Sciences; Psychology GA 093TR UT WOS:000315215700004 PM 23386480 ER PT J AU Mehl, HL Cotty, PJ AF Mehl, Hillary L. Cotty, Peter J. TI Nutrient Environments Influence Competition among Aspergillus flavus Genotypes SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID VEGETATIVE COMPATIBILITY GROUPS; AFLATOXIN B-1 PRODUCTION; CROP-ROTATION; SUBSTRATE UTILIZATION; CONIDIAL GERMINATION; REDUCTION STRATEGIES; SCLEROTIA PRODUCTION; COMMUNITY STRUCTURE; BIOLOGICAL-CONTROL; COST-EFFECTIVENESS AB The population dynamics of Aspergillus flavus, shaped in part by intraspecific competition, influence the likelihood and severity of crop aflatoxin contamination. Competition for nutrients may be one factor modulating intraspecific interactions, but the influences of specific types and concentrations of nutrients on competition between genotypes of A. flavus have not been investigated. Competition between paired A. flavus isolates on agar media was affected by varying concentrations of carbon (sucrose or asparagine) and nitrogen (nitrate or asparagine). Cocultivated isolate percentages from conidia and agar-embedded mycelia were quantified by measurements of isolate-specific single-nucleotide polymorphisms with quantitative pyrosequencing. Compositions and concentrations of nutrients influenced conidiation resulting from cocultivation, but the percentages of total conidia from each competing isolate were not predicted by sporulation of isolates grown individually. Success during sporulation did not reflect the outcomes of competition during mycelial growth, and the extents to which isolate percentages from conidia and mycelia differed varied among both isolate pairs and media. Whether varying concentrations of sucrose, nitrate, or asparagine increased, decreased, or had no influence on competitive ability was isolate dependent. Different responses of A. flavus isolates to nutrient variability suggest genotypes are adapted to different nutrient environments that have the potential to influence A. flavus population structure and the epidemiology of aflatoxin contamination. C1 [Mehl, Hillary L.; Cotty, Peter J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Cotty, Peter J.] ARS, USDA, Tucson, AZ USA. RP Cotty, PJ (reprint author), Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu OI Mehl, Hillary/0000-0001-8570-3562 FU Agricultural Research Service, U.S. Department of Agriculture, CRIS [5347-42000-020-00D] FX This work was supported by the Agricultural Research Service, U.S. Department of Agriculture, CRIS project 5347-42000-020-00D. NR 65 TC 4 Z9 4 U1 0 U2 26 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2013 VL 79 IS 5 BP 1473 EP 1480 DI 10.1128/AEM.02970-12 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 089ES UT WOS:000314893300007 PM 23263958 ER PT J AU Tang, JD Parker, LA Perkins, AD Sonstegard, TS Schroeder, SG Nicholas, DD Diehl, SV AF Tang, Juliet D. Parker, Leslie A. Perkins, Andy D. Sonstegard, Tad S. Schroeder, Steven G. Nicholas, Darrel D. Diehl, Susan V. TI Gene Expression Analysis of Copper Tolerance and Wood Decay in the Brown Rot Fungus Fibroporia radiculosa SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BASIDIOMYCETE GLOEOPHYLLUM-TRABEUM; OXALIC-ACID PRODUCTION; POSTIA-PLACENTA; FOMITOPSIS-PALUSTRIS; CHEMICAL-COMPOSITION; ROTTING FUNGI; LACCASE GENE; WHITE-ROT; DEGRADATION; CELLULOSE AB High-throughput transcriptomics was used to identify Fibroporia radiculosa genes that were differentially regulated during colonization of wood treated with a copper-based preservative. The transcriptome was profiled at two time points while the fungus was growing on wood treated with micronized copper quat (MCQ). A total of 917 transcripts were differentially expressed. Fifty-eight of these genes were more highly expressed when the MCQ was protecting the wood from strength loss and had putative functions related to oxalate production/degradation, laccase activity, quinone biosynthesis, pectin degradation, ATP production, cytochrome P450 activity, signal transduction, and transcriptional regulation. Sixty-one genes were more highly expressed when the MCQ lost its effectiveness (>50% strength loss) and had functions related to oxalate degradation; cytochrome P450 activity; H2O2 production and degradation; degradation of cellulose, hemicellulose, and pectin; hexose transport; membrane glycerophospholipid metabolism; and cell wall chemistry. Ten of these differentially regulated genes were quantified by reverse transcriptase PCR for a more in-depth study (4 time points on wood with or without MCQ treatment). Our results showed that MCQ induced higher than normal levels of expression for four genes (putative annotations for isocitrate lyase, glyoxylate dehydrogenase, laccase, and oxalate decarboxylase 1), while four other genes (putative annotations for oxalate decarboxylase 2, aryl alcohol oxidase, glycoside hydrolase 5, and glycoside hydrolase 10) were repressed. The significance of these results is that we have identified several genes that appear to be coregulated, with putative functions related to copper tolerance and/or wood decay. C1 [Tang, Juliet D.; Parker, Leslie A.; Perkins, Andy D.; Nicholas, Darrel D.; Diehl, Susan V.] Mississippi State Univ, Starkville, MS USA. [Sonstegard, Tad S.; Schroeder, Steven G.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD USA. RP Tang, JD (reprint author), Mississippi State Univ, Starkville, MS USA. EM jdt57@msstate.edu OI Schroeder, Steven/0000-0001-9103-5150 FU Lucas Biodeterioration Laboratory; Department of Forest Products; Mississippi State University; USDA Wood Utilization Research; Mississippi INBRE; National Center for Research Resources [5P20RR016476-11]; National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103476-11]; National Science Foundation [EPS 0903787, EPS 1006883] FX Funds for this work were provided by the Lucas Biodeterioration Laboratory, Department of Forest Products, Mississippi State University, and by USDA Wood Utilization Research. A.D.P. was supported by the Mississippi INBRE, funded by grants from the National Center for Research Resources (5P20RR016476-11) and the National Institute of General Medical Sciences (8 P20 GM103476-11) from the National Institutes of Health, and by the National Science Foundation under awards EPS 0903787 and EPS 1006883. NR 65 TC 9 Z9 9 U1 3 U2 29 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2013 VL 79 IS 5 BP 1523 EP 1533 DI 10.1128/AEM.02916-12 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 089ES UT WOS:000314893300013 PM 23263965 ER PT J AU Chen, HL Zhang, HY Zhu, KY Throne, J AF Chen, Haoliang Zhang, Hongyu Zhu, Kun Yan Throne, James TI Performance of diapausing parasitoid wasps, Habrobracon hebetor, after cold storage SO BIOLOGICAL CONTROL LA English DT Article DE Parasitoid; Braconidae; Reproductive diapause; Quality; Long-term storage; Biological control ID ALMOND MOTH LEPIDOPTERA; BRACON-HEBETOR; INSECT DIAPAUSE; HYMENOPTERA; TRICHOGRAMMATIDAE; HETEROPTERA; ACCLIMATION; TEMPERATURE; TOLERANCE; PYRALIDAE AB The ectoparasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) is an important potential biological control agent for lepidopterous pests of stored products. We investigated the effects of long-term cold storage of diapausing and nondiapausing H. hebetor on their performance after cold storage. Mortality during storage increased with increasing storage duration, and the mortality of diapausing females was lower than that of nondiapausing females after 8, 12, and 16 weeks of storage. Longevity, egg laying, number of progeny produced, and time to 50% egg laying were all reduced, as compared with the culture females when parasitoids were reared at conditions that do not induce diapause. But, for females reared at 20 degrees C at conditions that induce diapause, all of these quality parameters did not differ from those of culture insects when the storage duration was 8 weeks or less. The percentage of female F1 offspring was always lower for cold stored insects than for the culture insects. Presence of a male after cold storage did not impact any of the quality parameters measured. Thus, rearing parasitoids at 20 degrees C and 10L: 14D and then storing them for up to 8 weeks at 5 degrees C would produce parasitoids that are similar to culture parasitoids, except that the percentage of females is lower than that in the cultures (36% vs. 52%). (C) 2012 Elsevier Inc. All rights reserved. C1 [Chen, Haoliang; Zhang, Hongyu] Huazhong Agr Univ, Coll Plant Sci & Technol, Inst Urban & Hort Pests, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China. [Chen, Haoliang; Zhang, Hongyu] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Key Lab Insect Resource Applicat & Sustaina, Wuhan 430070, Hubei, Peoples R China. [Chen, Haoliang] Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Anhui, Peoples R China. [Chen, Haoliang; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Chen, Haoliang; Throne, James] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Zhang, HY (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Inst Urban & Hort Pests, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China. EM chl158184175@yahoo.com.cn; hongyu.zhang@mail.hzau.edu.cn; kzhu@ksu.edu; james.throne@ars.usda.gov FU China National Science and Technology Project of the 11th Five-Year Plan [2006BAI09B04-06, 2006BAD02A18-03]; Ear-marked Fund for Modern Agro-industry Technology Research System [CARS-27]; China Scholarship Council FX The authors thank Dr. Judy Johnson (USDA-ARS, Parlier, CA) for providing the Habrobracon hebetor and for reviewing an earlier version of this manuscript, and Joel Perez-Mendoza and Ann Redmon for their technical assistance. This research was supported by the China National Science and Technology Project of the 11th Five-Year Plan (2006BAI09B04-06 and 2006BAD02A18-03), the Ear-marked Fund for Modern Agro-industry Technology Research System (CARS-27) to H. Zhang, and a scholarship from the China Scholarship Council to H. Chen. This manuscript is Contribution No. 12-065-J from the Kansas Agricultural Experiment Station, Kansas State University. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 5 Z9 6 U1 1 U2 37 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2013 VL 64 IS 3 BP 186 EP 194 DI 10.1016/j.biocontrol.2012.11.007 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 093OJ UT WOS:000315200900002 ER PT J AU Zhang, YZ Hanula, JL O'Brien, J Horn, S Braman, K Sun, JH AF Zhang, Yanzhuo Hanula, James L. O'Brien, Joe Horn, Scott Braman, Kris Sun, Jianghua TI Evaluation of the impacts of herbivory by lace bugs on Chinese privet (Ligustrum sinense) survival and physiology SO BIOLOGICAL CONTROL LA English DT Article DE Privet; Lace bug; Biocontrol agent; Impact; Photosynthesis; Chlorophyll content ID BIOLOGICAL-CONTROL AGENT; MACFADYENA-UNGUIS-CATI; CARVALHOTINGIS-VISENDA; QUARANTINE CONDITIONS; EFFICACY ASSESSMENT; INSECT HERBIVORES; POTENTIAL IMPACT; RIPARIAN FORESTS; REPRODUCTION; TINGIDAE AB Biological control of Chinese privet, Ligustrum sinense, is the best long-term option for control of this widespread invasive plant in the southeastern USA. A pre-release efficacy assessment was conducted by testing the effects of damage caused by a lace bug, Leptoypha hospita, on potted privet plants in the laboratory. Inoculating 15 pairs of lace bug adults on plants resulted in a significantly higher defoliation rate and reduced leaf biomass by more than 59% compared to 0 and 3 lace bug pairs. Leaf biomass of plants inoculated with 3 and 9 pairs of lace bug did not differ significantly from control plants. The percentage of the total leaf area affected by lace bug feeding was positively correlated with the density of lace bugs inoculated. This was also evident by the reduced chlorophyll content of leaves exposed to 9 and 15 pairs of lace bugs and their offspring. Our tests showed that one generation of feeding by the lace bug caused significant defoliation as well as reduced photosynthetic activity of remaining leaves. Continuous long term feeding by the lace bug or other potential defoliating insects could result in suppression of Chinese privet populations and possibly reduction to desirable levels. (c) 2012 Elsevier Inc. All rights reserved. C1 [Zhang, Yanzhuo] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Hanula, James L.; O'Brien, Joe; Horn, Scott] USDA, Forest Serv, So Res Stn, Athens, GA 30602 USA. [Braman, Kris] Univ Georgia, Dept Entomol, Griffin, GA 30223 USA. [Sun, Jianghua] Chinese Acad Sci, Beijing 100101, Peoples R China. RP Zhang, YZ (reprint author), Univ Georgia, Dept Entomol, Athens, GA 30602 USA. EM yzzhang@uga.edu NR 59 TC 1 Z9 1 U1 1 U2 28 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2013 VL 64 IS 3 BP 299 EP 304 DI 10.1016/j.biocontrol.2012.12.003 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 093OJ UT WOS:000315200900017 ER PT J AU Cristofaro, M De Biase, A Smith, L AF Cristofaro, M. De Biase, A. Smith, L. TI Field release of a prospective biological control agent of weeds, Ceratapion basicorne, to evaluate potential risk to a nontarget crop SO BIOLOGICAL CONTROL LA English DT Article DE Host plant specificity; Common garden; Risk assessment; Weed ID HOST-SPECIFICITY TEST; CENTAUREA-SOLSTITIALIS ASTERACEAE; MELALEUCAE MOORE HEMIPTERA; TROPICAL SODA APPLE; YELLOW STARTHISTLE; BIOCONTROL AGENT; COLEOPTERA APIONIDAE; DIORHABDA-ELONGATA; UNITED-STATES; TAMARIX SPP. AB Ceratapion basicorne is a prospective biological control agent of yellow starthistle (Centaurea solstitialis), which is an invasive alien weed in the USA. Although the weevil has a strong preference for yellow starthistle, it has been reported to develop sometimes on safflower in larval transfer and no-choice experiments. Although safflower was not attacked by this insect in previous field experiments, a release permit was denied because of concern for risk to safflower. Adult C. basicorne were released in a field experiment in which two varieties of safflower were grown in solid blocks on either side of a small number of yellow starthistle plants. Plants were dissected at the time of weevil pupation. Immature insects were reared to adult stage on artificial diet or were preserved in acetone to identify by molecular genetic analysis. C. basicorne infested 54% of the yellow starthistle plants and 0% of 1021 safflower plants. A different weevil, Ceratapion orientale, infested 1.5% of the safflower plants. These results corroborate two other published field studies in which C. basicorne was not reared from safflower. The combined results of nine experiments provide a point estimate that the probability of attack is less than 0.00059, with 99.9% confidence that it is less than 0.0045. The consistency of results from field experiments in three countries and the absence of any report of this insect being reared from safflower in the field in the weevil's native range support the conclusion that this insect poses no significant risk to safflower. Published by Elsevier Inc. C1 [Cristofaro, M.] UTAGRI ECO, ENEA Casaccia, I-00123 Rome, Italy. [De Biase, A.] Univ Roma La Sapienza, Dept Biol & Biotechnol Charles Darwin, I-00185 Rome, Italy. [Smith, L.] USDA ARS, Albany, CA 94710 USA. RP Smith, L (reprint author), USDA ARS, 800 Buchanan St, Albany, CA 94710 USA. EM link.smith@ars.usda.gov NR 56 TC 4 Z9 4 U1 1 U2 34 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2013 VL 64 IS 3 BP 305 EP 314 DI 10.1016/j.biocontrol.2012.11.001 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 093OJ UT WOS:000315200900018 ER PT J AU Liu, HH Zhang, TY Liu, BY Liu, G Wilson, GV AF Liu, Honghu Zhang, Tianyu Liu, Baoyuan Liu, Gang Wilson, G. V. TI Effects of gully erosion and gully filling on soil depth and crop production in the black soil region, northeast China SO ENVIRONMENTAL EARTH SCIENCES LA English DT Article DE Soil erosion; Soil property; Soil and water conservation measures; Deposition ID RETREAT RATES; SEDIMENT; CS-137; SPAIN AB Gully erosion is a very serious problem in the black soil region of northeast China. Gully filling is often adopted for controlling gully erosion by local farmers and thus causes more serious soil erosion. In this study an ephemeral gully (EG, 74 m) and a classical gully (CG, 52 m) connected at the gully's headcut were selected as the study site. Two comparisons were made to explore the effects of gully erosion and the subsequent gully filling on soil depth and soybean yield: (1) soil depth between 81 sample points in the study site and 11 reference points along the same slope with the gully; (2) soybean yield between 81 sample points in the study site and 30 baseline locations near the study site. The results indicated that gully erosion caused the reduction of soil depth and soybean yield. Although filling gullies with soil from adjacent areas seemed to be an expedient way to remediate the gullies, it resulted in substantial soybean yield reduction. Gully erosion reduced the soil depth and soybean yield in 74.4 and 83.9 % of the study site, respectively. The soybean yield reduction ratio was 34.5 % for the whole study site and 2.6 % for the black soil region. Soil depth was the most important soil property indicator to reduce yield. Every 1 cm decrease in soil depth in the areas adjacent to gullies due to infilling activities resulted in a 2 % decrease in yield. More significant was the deposition of sediment from gully erosion, which completely eliminated soybean yield. Currently, effective soil and water conservation measures are not known and adopted by local farmers extensively. In the future, once some measures for preventing soil erosion, in particular gully erosion, were proved effective, these technologies should be disseminated among local farmers. C1 [Liu, Honghu; Zhang, Tianyu; Liu, Baoyuan; Liu, Gang] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Liu, Honghu] Changjiang River Sci Res Inst, Wuhan 430010, Peoples R China. [Liu, Honghu] State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [Wilson, G. V.] USDA ARS, Natl Sedimentat Lab, Watershed Phys Proc Res Unit, Oxford, MS 38655 USA. RP Liu, BY (reprint author), Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. EM honghu2005@gmail.com; baoyuan@bnu.edu.cn FU Ministry of Science and Technology [2007CB407204]; Key Laboratory of Earth Surface Processes and Resource Ecology [2009-KF-10]; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [10501-282]; Changjiang River Scientific Research Institute [CKSF 2012052/TB] FX The authors appreciate Dr. Yongguang Zhang, Dr. Chiyuan Miao and Dr. Xingwu Duan from Beijing Normal University for their assistance in the fieldwork. The study was supported by Ministry of Science and Technology 973 plan project (2007CB407204), Open Fund of the Key Laboratory of Earth Surface Processes and Resource Ecology (2009-KF-10), Open Fund of the State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau (10501-282) and Innovation Project of Changjiang River Scientific Research Institute (CKSF 2012052/TB). NR 35 TC 6 Z9 8 U1 5 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1866-6280 J9 ENVIRON EARTH SCI JI Environ. Earth Sci. PD MAR PY 2013 VL 68 IS 6 BP 1723 EP 1732 DI 10.1007/s12665-012-1863-0 PG 10 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA 095LA UT WOS:000315334700018 ER PT J AU Aronstein, KA Webster, TC Saldivar, E AF Aronstein, K. A. Webster, T. C. Saldivar, E. TI A serological method for detection of Nosema ceranae SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Apis mellifera; enzyme-linked immunosorbent assay; molecular tool; Nosema ceranae ID APIS-MELLIFERA; INFECTION; HONEYBEE AB Aims We developed a new method for detection of the intracellular parasite, Nosema ceranae, one of the most economically devastating pathogens of the honeybee. Methods and Results The SWP-32 antibody was used for the development of an enzyme-linked immunosorbent assay (ELISA). We also compared the efficiency of this ELISA to microscopy and quantitative real-time (qRT) PCR, the methods currently in use. Conclusions ELISA is comparable in sensitivity with the qRT-PCR, less expensive and faster. When this method is commercialized and made available to bee-keepers, it will allow them to make informed decisions for the application of in-hive chemicals. Hence, bee-keepers may be able to determine when treatments for control of N.ceranae are unnecessary and reduce the cost, time and possible side effects of these treatments. Significance and Impact of the Study This assay provides the first serological method for detection of N.ceranae in bee colonies, which is as sensitive as DNA amplification. It can be easily adopted for both laboratory and field applications. C1 [Aronstein, K. A.; Saldivar, E.] USDA ARS, Honey Bee Res Unit, Weslaco, TX 78596 USA. [Webster, T. C.] Kentucky State Univ, Coll Agr Food Sci & Sustainable Syst, Frankfort, KY USA. RP Aronstein, KA (reprint author), USDA ARS, Honey Bee Res Unit, 2413 E Hwy 83, Weslaco, TX 78596 USA. EM kate.aronstein@ars.usda.gov FU USDA National Institute of Food and Agriculture [2009-85118-05718] FX We thank Dr Lee Solter (University of Illinois, IL, USA) for providing the control strain of N. ceranae. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2009-85118-05718 from the USDA National Institute of Food and Agriculture, awarded in part to K.A.A. and T.C.W. NR 10 TC 2 Z9 2 U1 4 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAR PY 2013 VL 114 IS 3 BP 621 EP 625 DI 10.1111/jam.12066 PG 5 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 093JG UT WOS:000315187400002 PM 23163924 ER PT J AU Pridgeon, JW Klesius, PH Garcia, JC AF Pridgeon, J. W. Klesius, P. H. Garcia, J. C. TI Identification and virulence of Chryseobacterium indologenes isolated from diseased yellow perch (Perca flavescens) SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Chryseobacterium indologenes; pathogen; virulence; yellow perch ID SP NOV.; WATER; FISH AB Aim To identify pathogen of diseased yellow perch and determine their virulence. Methods and Results Fifteen Gram-negative bacterial isolates were recovered from the skin lesions of diseased yellow perch (Perca flavescens). Based on API 20NE test, ten isolates were found to share 67 center dot 299 center dot 9% homologies with Chryseobactertium indologenes. Based on fatty acid methyl ester analysis, 13 isolates were found to share similarities with C.indologenes and other species of Chryseobacterium. Based on sequencing results of partial 16S rRNA gene, 13 isolates shared 99% identities (e value=2e50) with the 16S rRNA sequence of C.indologenes (GenBank HQ259684). Based on the 16S23S rRNA intergenic spacer region (ISR) sequence, the 13 isolates shared 88% identity (e value=1e165) with the 16S23S ISR sequence of C.indologenes (GenBank EU014570). T-coffee multiple sequence alignment revealed that the partial 16S rRNA or the 16S23S ISR sequence of the 13 isolates shared 100% identity with each other. When healthy yellow perch were exposed to the 15 isolates by bath immersion (c.6x107CFUml1 for 1h), only C.indologenes isolates killed 1020% of fish, whereas other isolates were avirulent. When yellow perch were exposed to C.indologenes by intraperitoneal injection, mortality was dose dependent, with LD50 and LD95 values of 1 center dot 5x108 and 3 center dot 2x108CFU per fish, respectively. Conclusions Chryseobactertium indologenes could be pathogenic to yellow perch. Significance and Impact of the Study This is the first report on the isolation of C.indologenes from diseased yellow perch. Virulence studies suggested that C.indologenes could become pathogenic to yellow perch. C1 [Pridgeon, J. W.; Klesius, P. H.; Garcia, J. C.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Pridgeon, JW (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM julia.pridgeon@ars.usda.gov FU USDA/ARS CRIS [6420-32000-024-00D] FX We thank Drs Dunhua Zhang (USDA-ARS) and Victor Panangala (USDA collaborator) for critical reviews of the manuscript. We thank Dr Brian Scheffler and Fanny Liu (USDA-ARS-Catfish Genetics Research Unit) for their excellent sequencing work. We thank Beth Peterman (USDA-ARS) for her excellent technical support. This study was supported by the USDA/ARS CRIS project no. 6420-32000-024-00D. The use of trade, firm or corporate names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 20 TC 5 Z9 5 U1 2 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAR PY 2013 VL 114 IS 3 BP 636 EP 643 DI 10.1111/jam.12070 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 093JG UT WOS:000315187400004 PM 23164054 ER PT J AU McQuate, GT AF McQuate, Grant T. TI Timing of onset of evening activity of adult Chinese rose beetles (Coleoptera: Scarabaeidae) SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Chinese rose beetle; Adoretus sinicus; Scarabaeidae; Evening activity onset; Light avoidance AB Adult Chinese rose beetles, Adoretus sinicus (Burmeister) (Coleoptera: Scarabaeidae: Adoretini), present in China, Taiwan, Indonesia, Cambodia, Laos, Singapore, Thailand, Vietnam, the Marianas Islands, the Caroline Islands, and the Hawaiian Islands, are nighttime defoliators that feed on a wide variety of plant species. It has recently been demonstrated that illumination of plants at dusk has the potential to discourage feeding by adult Chinese rose beetles on the illuminated plants. To effectively use lighting to minimize defoliation of host plants, it is critical to know the timing of the initial host plant colonization by the beetles to ensure that illumination is initiated before host plant colonization begins. Adult Chinese rose beetles were observed to colonize host plants at dusk, with initiation of beetle colonization averaging more than 21 min after sunset, with the earliest observed beetle colonization occurring 11 mm after sunset. These times corresponded to an average light level of 7.0 lux at the first colonization and the earliest first colonization occurring at 26.9 lux. Based on these results, use of lighting to minimize defoliation of host plants should be initiated at about sunset in order to discourage colonization (and associated defoliation) by adult Chinese rose beetles. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved. C1 USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP McQuate, GT (reprint author), USDA ARS, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM grant.mcquate@ars.usda.gov NR 9 TC 1 Z9 1 U1 0 U2 12 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD MAR PY 2013 VL 16 IS 1 BP 1 EP 4 DI 10.1016/j.aspen.2012.08.003 PG 4 WC Entomology SC Entomology GA 092MM UT WOS:000315126700001 ER PT J AU Wee, SL Shelly, T AF Wee, Suk Ling Shelly, Todd TI Capture of Bactrocera fruit flies in traps baited with liquid versus solid formulations of male lures in Malaysia SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Detection; Surveillance; Parapheromone; Invasive species ID METHYL EUGENOL; CUE-LURE; INSECTICIDE DISPENSERS; DIPTERA TEPHRITIDAE; FLY; HAWAII; CUCURBITAE; MANAGEMENT; COMPLEX AB Approximately 70 species of Bactrocera fruit flies (Diptera: Tephritidae) are polyphagous economic pests that attack many important agricultural crops. Several of these Bactrocera species are also highly invasive, and many countries operate continuous, large-scale trapping programs to detect incipient infestations. Detection programs rely heavily on traps baited with male lures, with males of some species responding to raspberry ketone (RK; or its synthetic analogue cue-lure [CL]) and males of other species responding to methyl eugenol (ME). These lures (plus naled, an insecticide) are currently applied as liquids, although this procedure is time-consuming and may expose workers to health risks. Recent field tests, conducted largely in Hawaii, have shown that traps baited with a solid formulation (termed a wafer) that contains both RK and ME (plus dichlorvos, an insecticide) capture as many or more B. dorsalis (Hendel) and B. cucurbitae (Coquillett) males as traps baited with the standard liquid lures. While these results are promising, a more complete evaluation of the solid formulation requires testing in a region with a diverse assemblage of Bactrocera species, since interspecific variation in male response to lures has been reported. The objective of the present investigation was to assess the relative effectiveness of liquid versus solid formulations of male lures in Malaysia, a country known to harbor a large assemblage of Bactrocera species. Based on a 12-week sampling period, we found that, contrary to the Hawaiian results, traps baited with the wafer captured significantly fewer males than traps baited with liquid lures for all five ME-responding taxa analyzed and for one of the three RK/CL-responding species analyzed. Possible explanations for the discrepancy between these and earlier findings are offered. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved. C1 [Wee, Suk Ling] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Environm & Nat Resource Sci, Bangi 43600, Selangor Darul, Malaysia. [Shelly, Todd] USDA APHIS, Waimanalo, HI 96795 USA. RP Shelly, T (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 26 TC 0 Z9 1 U1 1 U2 41 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD MAR PY 2013 VL 16 IS 1 BP 37 EP 42 DI 10.1016/j.aspen.2012.09.006 PG 6 WC Entomology SC Entomology GA 092MM UT WOS:000315126700007 ER PT J AU Kim, KS Sappington, TW AF Kim, Kyung Seok Sappington, Thomas W. TI Population genetics strategies to characterize long-distance dispersal of insects SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Review DE Population genetics; Population assignment; Dispersal; Insect pests; Boll weevil; Anthonomus grandis ID BOLL-WEEVILS COLEOPTERA; DIABROTICA-VIRGIFERA-VIRGIFERA; WESTERN CORN-ROOTWORM; MULTIPLE TRANSATLANTIC INTRODUCTIONS; ANTHONOMUS-GRANDIS COLEOPTERA; MICROSATELLITE MARKERS; SOUTH-AMERICA; MULTILOCUS GENOTYPES; MITOCHONDRIAL-DNA; MIGRATION RATES AB Population genetics strategies offer an alternative and powerful approach for obtaining information about long-distance movement, and have been widely used for examining patterns and magnitude of insect dispersal over geographic and temporal scales. Such strategies are based on the principle that genetic divergence between local populations reflects the interplay between genetic drift and gene flow, and thus can function as an indicator of dispersal capacity. Relatively new approaches for inferring population history are widely applicable for documenting introduction routes of invasive or quarantine species. These approaches are based on genetic variability calculated from changes in gene frequency of subpopulations, measured using molecular genetic markers. Inferences from population genetics can supplement and corroborate conventional observational approaches for characterizing insect dispersal and have provided important clues to many questions raised in the field of behavior and ecology of insects. Here, we summarize our work on the boll weevil as a case study to illustrate the kinds of information on dispersal capacity and dispersal patterns that can be obtained from population genetics techniques that would be difficult or impossible to acquire in other ways. Then we provide examples of how the molecular markers and population genetics tools have been applied to answer immediate questions of relevance to eradication program managers. Though the latter are idiosyncratic to this particular pest, they demonstrate the kinds and range of problems that can be addressed in other systems through application of population genetics strategies. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved. C1 [Kim, Kyung Seok] Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea. [Sappington, Thomas W.] ISU, Genet Lab, CICGRU, USDA ARS, Ames, IA 50011 USA. RP Sappington, TW (reprint author), ISU, Genet Lab, CICGRU, USDA ARS, Ames, IA 50011 USA. EM kyungkim@snu.ac.kr; Tom.Sappington@ars.usda.gov NR 126 TC 5 Z9 6 U1 6 U2 84 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD MAR PY 2013 VL 16 IS 1 BP 87 EP 97 DI 10.1016/j.aspen.2012.11.004 PG 11 WC Entomology SC Entomology GA 092MM UT WOS:000315126700015 ER PT J AU Connor, EE Kahl, S Elsasser, TH Baldwin, RL Fayer, R Santin-Duran, M Sample, GL Evock-Clover, CM AF Connor, E. E. Kahl, S. Elsasser, T. H. Baldwin, R. L. Fayer, R. Santin-Duran, M. Sample, G. L. Evock-Clover, C. M. TI Glucagon-like peptide 2 therapy reduces negative effects of diarrhea on calf gut SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE calf diarrhea; coccidiosis; glucagon-like peptide 2; nitrotyrosine ID CALVES; ADAPTATION; CHALLENGE; NITRATION; INFUSION; GROWTH; GLP-2 AB Damage to the intestinal epithelium reduces nutrient absorption and animal growth, and can have negative long-term health effects on livestock. Because the intestinotropic hormone glucagon-like peptide 2 (GLP-2) has been shown to contribute to gut integrity, reduce inflammation, and improve nutrient absorption, the present study was designed to determine whether administration of GLP-2 to calves with coccidiosis in the first month of life affects intestinal growth and mediates negative effects of the proinflammatory response. Holstein bull calves (n = 19) were assigned to 4 treatment groups of 4 to 5 calves each: (1) infected with Eimeria bovis, GLP-2 treated; (2) noninfected, GLP-2 treated; (3) infected with E. bovis, buffer treated; and (4) noninfected, buffer treated. Infected calves received 100,000 to 200,000 sporulated E. bovis oocysts suspended in milk replacer on d 0 of the study. On d 18, calves in the GLP-2 groups received a subcutaneous injection of 50 p,g of bovine GLP-2/kg of body weight twice daily for 10 d, and calves in the buffer-treated groups received an equivalent volume of sodium bicarbonate buffer only. On d 28, calves were slaughtered 2 h after injection of 5-bromo-2'-deoxyuridine (BrdU). Intestinal tissues were measured and villus height, crypt depth, and BrdU immunostaining were evaluated in segments of the small intestine. Nitrotyrosine immunostaining, a measure of nitro-oxidative damage, was evaluated in the ileum and cecum. No GLP-2 treatment by E. bovis infection interaction was observed for any parameter measured, with the exception of nitrotyrosine immunostaining in the cecum. Large intestinal weight was greater in infected than noninfected calves and with GLP-2 treatment relative to buffer treatment. Calves that received GLP-2 also had greater small intestinal weight but no difference in cell proliferation, as assessed by BrdU labeling, relative to buffer-treated calves. No treatment effects were detected for villus height, crypt depth, or villus height:crypt depth ratio in segments of the small intestine. Protein tyrosine nitration was over 3-fold greater in the ileum and cecum of infected calves relative to noninfected calves, and GLP-2 therapy reduced tyrosine nitration in infected calves by 47% in the ileum and 69% in the cecum relative to buffer-treated calves. Treatment with GLP-2 promotes intestinal growth in neonatal calves and reduces the detrimental effects of nitro-oxidative stress in the ileocecum of calves with coccidiosis. C1 [Connor, E. E.; Kahl, S.; Elsasser, T. H.; Baldwin, R. L.; Evock-Clover, C. M.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. [Fayer, R.; Santin-Duran, M.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Sample, G. L.] ARS, Vet Serv, USDA, Beltsville, MD 20705 USA. RP Connor, EE (reprint author), ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. EM erin.connor@ars.usda.gov NR 23 TC 5 Z9 5 U1 2 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2013 VL 96 IS 3 BP 1793 EP 1802 DI 10.3168/jds.2012-6216 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 091PG UT WOS:000315061700047 PM 23312992 ER PT J AU VanRaden, PM Cooper, TA Wiggans, GR O'Connell, JR Bacheller, LR AF VanRaden, P. M. Cooper, T. A. Wiggans, G. R. O'Connell, J. R. Bacheller, L. R. TI Confirmation and discovery of maternal grandsires and great-grandsires in dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE haplotype; genomics; pedigree discovery; pedigree reconstruction ID SINGLE-NUCLEOTIDE POLYMORPHISMS; PEDIGREE RECONSTRUCTION; MICROSATELLITE MARKERS; GENOMIC EVALUATION; PARENTAGE; IDENTIFICATION; SELECTION; GENOTYPES; ERRORS; IMPACT AB Selection, mating, and improvement of dairy animals have required accurate pedigrees. Genomic tools allow paternal ancestors to be easily confirmed or discovered because most sires are genotyped for many markers, but maternal ancestors are more difficult to discover because most female ancestors are not genotyped. Three methods to discover maternal grandsires (MGS) were developed and compared. Conflicts were counted one single nucleotide polymorphism (SNP) at a time between genotypes of the animal and potential MGS (duo method) or also using the sire's genotype (trio method). Alternatively, haplotypes of a potential MGS were matched to the animal's maternal haplotype, obtained by using linkage across loci (HAP method). The duo and trio methods can be performed as soon as a genotype is received because no imputation is required. The HAP method improved accuracy because genotypes with 2,683 (3K) SNP were imputed to the 45,187 (50K) SNP used for genomic evaluation. The HAP method was tested using modified pedigrees with 5% of true MGS replaced by a random genotyped bull from the same birth year and 5% of MGS set to missing for 4,134 Holsteins, 552 Jerseys, and 142 Brown Swiss that had confirmed, genotyped sires. Those same animals were used to test the duo and trio methods, except that some animals had multiple genotypes and imputed dams were excluded. Accuracy measured how often the correct MGS was selected from among 12,152 genotyped Holstein, 2,265 Jersey, and 1,605 Brown Swiss potential MGS. Accuracies were 61, 60, and 65%, respectively, with the duo method; 95, 91, and 94% with the trio method; and 97, 95, and 97% with the HAP method. Accuracy of the duo method was poor (only 52% for animals genotyped with 3K and 65% with 50K) because additional information from the paternal genotype is not used. Accuracy of the trio method was 97% with 50K but only 78% with 3K because the missing SNP were not imputed. Accuracy of the HAP method was 94% with 3K genotypes, 98% with 50K, and 92% with nongenotyped, imputed dams. When the HAP method was extended to great-grandsires, the accuracy of maternal great-grandsire discovery was 92% for 652 Holsteins, 95% for 33 Jerseys, and 85% for 20 Brown Swiss. Accuracy was even higher using simulated genotypes. Because most dairy bulls over several generations have been genotyped, percentages of haplotypes shared with candidate males can accurately confirm, correct, or discover the sires, MGS, and even more distant ancestors of most animals. C1 [VanRaden, P. M.; Cooper, T. A.; Wiggans, G. R.; Bacheller, L. R.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [O'Connell, J. R.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. RP VanRaden, PM (reprint author), USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. EM Paul.VanRaden@ars.usda.gov NR 25 TC 6 Z9 6 U1 0 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2013 VL 96 IS 3 BP 1874 EP 1879 DI 10.3168/jds.2012-6176 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 091PG UT WOS:000315061700056 PM 23332849 ER PT J AU Fogg, AM George, TL Purcell, KL AF Fogg, Alissa M. George, T. Luke Purcell, Kathryn L. TI Intersexual variation in the foraging ecology of sexually monochromatic Western Wood-Pewees SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE Contopus sordidulus; foraging rate; habitat selection; montane meadow; perch use; Sierra Nevada mountains; tyrant flycatcher ID PROTHONOTARY WARBLERS; INSECTIVOROUS BIRDS; FOOD AVAILABILITY; HABITAT STRUCTURE; ENERGETIC STRESS; BREEDING-SEASON; FOREST BIRDS; MASS-LOSS; BEHAVIOR; FLYCATCHERS AB Investigators generally pool observations of males and females in studies of the foraging behavior of sexually monochromatic songbirds. However, such pooling can obscure possible intersexual differences. We compared the foraging behavior of male and female Western Wood-Pewees (Contopus sordidulus), a sexually monochromatic species, in the Sierra Nevada Mountains of California during the breeding seasons of 2007 and 2008. We recorded 143 foraging observations (male N= 74, female N= 69). Overall, mean foraging rates of females (2.8 attacks/min) were higher (P<0.001) than those of males (1.1 attacks/min). In addition, female foraging rates were significantly higher during incubation than during the nest building, nestling, and fledgling periods. When foraging, males perched higher above ground than females (means = 17.1 and 6.7 m, respectively). Differences between male and female Western Wood-Pewees in foraging rates and perch heights suggest that males may spend more time on vigilance while females focus on foraging quickly during incubation and when feeding nestlings. Because metrics such as foraging attack rates are sometimes used as indicators of habitat quality and we found that rates can differ between the sexes and among nesting stages, investigators should consider the possibility of such differences when assessing habitat quality, especially for sexually monochromatic species of birds. RESUMEN Investigadores generalmente agrupan observaciones de machos y hembras en estudios de comportamientos de busqueda de alimento en aves paseriformes sexualmente monocromaticas. Sin embargo, esta agregacion puede estar ocultando posibles diferencias entre los sexos. Comparamos el comportamiento de busqueda de alimento de machos y hembras de Contopus sordidulus, una especie sexualmente monocromatica, en las montanas de la Sierra Nevada de California durante la temporada reproductiva del 2007 y 2008. Colectamos 143 observaciones de busqueda de alimento (machos N= 74, hembras N= 69). En total, la tasa promedio de busqueda de alimento de las hembras (2.8 ataques/min) fue mayor (P < 0.001) que la de los machos (1.1 ataques/min). Adicionalmente, la tasa de busqueda de alimento por parte de las hembras fue significativamente mas altas durante la incubacion que durante los periodos de construccion del nido, polluelos y volantones. Cuando buscan alimentos, los machos se perchan a mayores alturas respecto al suelo en comparacion a las hembras (promedio = 17.1 y 6.7 m, respectivamente). Diferencias entre machos y hembras de C. Sordidulus en tasas de busqueda de alimento y altura de perchas sugiere que los machos pueden estar pasando mas tiempo vigilando mientras que las hembras se enfocan en buscar alimento rapidamente durante la incubacion y cuando estan alimentando a los polluelos. Debido a que medidas metricas como tasas de ataque durante la busqueda de alimento son usadas ocasionalmente como indicadores de calidad del habitat, y debido a que encontramos que las tasa de busqueda de alimento pueden diferir entre sexos y etapas durante la anidacion, investigadores deberian considerar la posibilidad de la existencia de estas diferencias cuando evaluen la calidad del habitat, especialmente en especies de aves sexualmente monocromaticas. C1 [Fogg, Alissa M.; George, T. Luke] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Purcell, Kathryn L.] US Forest Serv, USDA, Pacific Southwest Res Stn, Sierra Nevada Res Ctr, Fresno, CA 93710 USA. RP Fogg, AM (reprint author), PRBO Conservat Sci, 3820 Cypress Dr,Suite 11, Petaluma, CA 94954 USA. EM afogg@prbo.org RI Purcell, Kathryn/S-2592-2016 FU U.S. Forest Service Pacific Southwest Research Station; National Fish and Wildlife Foundation; Eureka Rotary Club; Humboldt State University Wildlife graduate student society FX We thank the U.S. Forest Service Pacific Southwest Research Station, the National Fish and Wildlife Foundation, the Eureka Rotary Club, and Humboldt State University Wildlife graduate student society for providing funding. We specifically thank D. Drynan for logistical support and S. Byrd for providing access to Southern California Edison land. All field methods were approved by the Humboldt State University Institutional Animal Care and Use Committee. We are grateful for comments from A. Jahn, R. Burnett, L. J. Roberts, and three anonymous reviewers. NR 48 TC 2 Z9 2 U1 0 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 EI 1557-9263 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD MAR PY 2013 VL 84 IS 1 BP 40 EP 48 DI 10.1111/jofo.12004 PG 9 WC Ornithology SC Zoology GA 095IX UT WOS:000315329000005 ER PT J AU Kelley, DS Adkins, Y Reddy, A Woodhouse, LR Mackey, BE Erickson, KL AF Kelley, Darshan S. Adkins, Yuriko Reddy, Aurosis Woodhouse, Leslie R. Mackey, Bruce E. Erickson, Kent L. TI Sweet Bing Cherries Lower Circulating Concentrations of Markers for Chronic Inflammatory Diseases in Healthy Humans SO JOURNAL OF NUTRITION LA English DT Article ID CORONARY HEART-DISEASE; S100A12 EN-RAGE; TART CHERRY; CARDIOVASCULAR-DISEASE; GENE-EXPRESSION; RISK; ANTIOXIDANT; POLYPHENOLS; FRUIT; ANTHOCYANINS AB A limited number of studies have demonstrated that some modulators of inflammation can be altered by the consumption of sweet cherries: We have taken a proteomics approach to determine the effects of dietary cherries on targeted gene expression. The purpose was then to determine changes caused by cherry consumption in the plasma concentrations of multiple biomarkers for several chronic inflammatory diseases in healthy humans with modestly elevated C-reactive protein (CRP; range, 1-14 mg/L; mean, 3.5 mg/L; normal, <1.0 mg/L). Eighteen men and women (45-61 y) supplemented their diets with Bing sweet cherries (280 g/d) for 28 d. Fasting blood samples were taken before the start of consuming the cherries (study d 7), 28 d after the initiation of cherry supplementation (d 35), and 28 d after the discontinuation (d 63). Of the 89 biomarkers assessed, cherry consumption for 28 d altered concentrations of 9, did not change those of 67, and the other 13 were below the detection limits. Cherry consumption decreased (P < 0.05) plasma concentrations of extracellular newly identified ligand for the receptor for advanced glycation end products (29.0%), CRP (20.1%), ferritin (20.3%), plasminogen activator inhibitor-1 (19.9%), endothelin-1 (13.7%), epidermal growth factor (13.2%), and IL-18 (8.1%) and increased that of IL-1 receptor antagonist (27.9%) compared with corresponding values on study d 7. The ferritin concentration continued to decrease between d 35 and 63 and it was significantly lower on d 63 than on d 7. Because the participants in this study were healthy, no clinical pathology end points were measured. However, results from the present study demonstrate that cherry consumption selectively reduced several biomarkers associated with inflammatory diseases. J. Nutr. 143: 340-344, 2013. C1 [Kelley, Darshan S.; Adkins, Yuriko; Reddy, Aurosis; Woodhouse, Leslie R.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Kelley, Darshan S.; Adkins, Yuriko; Reddy, Aurosis; Woodhouse, Leslie R.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Mackey, Bruce E.] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Erickson, Kent L.] Univ Calif Davis, Sch Med, Dept Cell Biol & Human Anat, Davis, CA 95616 USA. RP Kelley, DS (reprint author), ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. EM darshan.kelley@ars.usda.gov FU USDA, Agricultural Research Service; Washington State Fruit Commission, Yakima [WA 98901]; California Cherry Advisory Board FX Supported in part by the USDA, Agricultural Research Service, and the Washington State Fruit Commission, Yakima, WA 98901. The initial study was supported by the California Cherry Advisory Board. Reference to a company or product name does not imply approval or recommendation of the product by the USDA to the exclusion of others that may be suitable. USDA is an equal opportunity employer and provider. NR 42 TC 11 Z9 12 U1 0 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 2013 VL 143 IS 3 BP 340 EP 344 DI 10.3945/jn.112.171371 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 093EC UT WOS:000315173500013 PM 23343675 ER PT J AU Hruby, A Ngwa, JS Renstrom, F Wojczynski, MK Ganna, A Hallmans, G Houston, DK Jacques, PF Kanoni, S Lehtimaki, T Lemaitre, RN Manichaikul, A North, KE Ntalla, I Sonestedt, E Tanaka, T van Rooij, FJA Bandinelli, S Djousse, L Grigoriou, E Johansson, I Lohman, KK Pankow, JS Raitakari, OT Riserus, U Yannakoulia, M Zillikens, MC Hassanali, N Liu, YM Mozaffarian, D Papoutsakis, C Syvanen, AC Uitterlinden, AG Viikari, J Groves, CJ Hofman, A Lind, L McCarthy, MI Mikkila, V Mukamal, K Franco, OH Borecki, IB Cupples, LA Dedoussis, GV Ferrucci, L Hu, FB Ingelsson, E Kahonen, M Kao, WHL Kritchevsky, SB Orho-Melander, M Prokopenko, I Rotter, JI Siscovick, DS Witteman, JCM Franks, PW Meigs, JB McKeown, NM Nettleton, JA AF Hruby, Adela Ngwa, Julius S. Renstrom, Frida Wojczynski, Mary K. Ganna, Andrea Hallmans, Goran Houston, Denise K. Jacques, Paul F. Kanoni, Stavroula Lehtimaki, Terho Lemaitre, Rozenn N. Manichaikul, Ani North, Kari E. Ntalla, Ioanna Sonestedt, Emily Tanaka, Toshiko van Rooij, Frank J. A. Bandinelli, Stefania Djousse, Luc Grigoriou, Efi. Johansson, Ingegerd Lohman, Kurt K. Pankow, James S. Raitakari, Olli T. Riserus, Ulf Yannakoulia, Mary Zillikens, M. Carola Hassanali, Neelam Liu, Yongmei Mozaffarian, Dariush Papoutsakis, Constantina Syvanen, Ann-Christine Uitterlinden, Andre G. Viikari, Jorma Groves, Christopher J. Hofman, Albert Lind, Lars McCarthy, Mark I. Mikkila, Vera Mukamal, Kenneth Franco, Oscar H. Borecki, Ingrid B. Cupples, L. Adrienne Dedoussis, George V. Ferrucci, Luigi Hu, Frank B. Ingelsson, Erik Kahonen, Mika Kao, W. H. Linda Kritchevsky, Stephen B. Orho-Melander, Marju Prokopenko, Inga Rotter, Jerome I. Siscovick, David S. Witteman, Jacqueline C. M. Franks, Paul W. Meigs, James B. McKeown, Nicola M. Nettleton, Jennifer A. TI Higher Magnesium Intake Is Associated with Lower Fasting Glucose and Insulin, with No Evidence of Interaction with Select Genetic Loci, in a Meta-Analysis of 15 CHARGE Consortium Studies SO JOURNAL OF NUTRITION LA English DT Article ID TYPE-2 DIABETES-MELLITUS; WHOLE-GRAIN INTAKE; DOUBLE-BLIND; COFFEE CONSUMPTION; DIETARY MAGNESIUM; ENVIRONMENT INTERACTIONS; CARDIOVASCULAR-DISEASE; NONDIABETIC SUBJECTS; METABOLIC SYNDROME; PROSPECTIVE COHORT AB Favorable associations between magnesium intake and glycemic traits, such as fasting glucose and insulin, are observed in observational and clinical studies, but whether genetic variation affects these associations is largely unknown. We hypothesized that single nucleotide polymorphisms (SNPs) associated with either glycemic traits or magnesium metabolism affect the association between magnesium intake and fasting glucose and insulin. Fifteen studies from the CHARGE (Cohorts for Heart and Aging Research in Genomic Epidemiology) Consortium provided data from up to 52,684 participants of European descent without known diabetes. In fixed-effects meta-analyses, we quantified 1) cross-sectional associations of dietary magnesium intake with fasting glucose (mmol/L) and insulin (In-pmol/L) and 2) interactions between magnesium intake and SNPs related to fasting glucose (16 SNPs), insulin (2 SNPs), or magnesium (8 SNPs) on fasting glucose and insulin. After adjustment for age, sex, energy intake, BMI, and behavioral risk factors, magnesium (per 50-mg/d increment) was inversely associated with fasting glucose [beta = -0.009 mmol/L (95% CI: -0.013, -0.005), P< 0.0001] and insulin (-0.020 In-pmo/L (95% CI: -0.024, -0.017), P< 0.0001]. No magnesium-related SNP or interaction between any SNP and magnesium reached significance after correction for multiple testing. However, rs2274924 in magnesium transporter-encoding TRPM6 showed a nominal association (uncorrected P= 0.03) with glucose, and rs11558471 in SLC30A8and rs3740393 near CNNM2showed a nominal interaction (uncorrected, both P = 0.02) with magnesium on glucose. Consistent with other studies, a higher magnesium intake was associated with lower fasting glucose and insulin. Nominal evidence of TRPM6 influence and magnesium interaction with select loci suggests that further investigation is warranted. J. Nutr. 143: 345-353, 2013. C1 [Hruby, Adela; Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ngwa, Julius S.; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [Renstrom, Frida; Hu, Frank B.; Franks, Paul W.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Renstrom, Frida; Sonestedt, Emily; Orho-Melander, Marju; Franks, Paul W.] Lund Univ, Dept Clin Sci, Malmo, Sweden. [Renstrom, Frida; Franks, Paul W.] Umea Univ, Dept Publ Hlth & Clin Med, Umea, Sweden. [Wojczynski, Mary K.; Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Ganna, Andrea; Ingelsson, Erik] Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden. [Hallmans, Goran] Umea Univ, Dept Publ Hlth & Clin Med, Umea, Sweden. [Houston, Denise K.; Kritchevsky, Stephen B.] Wake Forest Sch Med, Dept Internal Med, Winston Salem, NC USA. [Kanoni, Stavroula] Wellcome Trust Sanger Inst, Hinxton, England. [Kanoni, Stavroula; Grigoriou, Efi.; Yannakoulia, Mary; Papoutsakis, Constantina; Dedoussis, George V.] Harokopio Univ Athens, Dept Nutr & Dietet, Athens, Greece. [Lehtimaki, Terho] Fimlab Labs, Tampere, Finland. [Lehtimaki, Terho] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland. [Lehtimaki, Terho] Tampere Univ Hosp, Tampere, Finland. [Lemaitre, Rozenn N.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Manichaikul, Ani] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [Manichaikul, Ani] Univ Virginia, Dept Publ Hlth Sci, Div Biostat & Epidemiol, Charlottesville, VA USA. [North, Kari E.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [North, Kari E.] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA. [Ntalla, Ioanna; Tanaka, Toshiko; Ferrucci, Luigi] NIA, Clin Res Branch, Baltimore, MD 21224 USA. [van Rooij, Frank J. A.; Zillikens, M. Carola; Uitterlinden, Andre G.; Hofman, Albert; Franco, Oscar H.; Witteman, Jacqueline C. M.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [van Rooij, Frank J. A.; Zillikens, M. Carola; Uitterlinden, Andre G.; Hofman, Albert; Franco, Oscar H.; Witteman, Jacqueline C. M.] Netherlands Consortium Hlth Aging, Netherlands Genom Initiat, Leiden, Netherlands. [Bandinelli, Stefania] Azienda Sanit Firenze, Geriatr Unit, Florence, Italy. [Djousse, Luc] Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA. [Djousse, Luc; Meigs, James B.] Harvard Univ, Sch Med, Boston, MA USA. [Djousse, Luc] Boston Vet Affairs Healthcare Syst, Massachusetts Vet Epidemiol & Res Informat Ctr, Boston, MA USA. [Djousse, Luc] Boston Vet Affairs Healthcare Syst, Ctr Geriatr Res Educ & Clin, Boston, MA USA. [Johansson, Ingegerd] Umea Univ, Dept Odontol, Umea, Sweden. [Lohman, Kurt K.] Wake Forest Sch Med, Dept Biostat Sci, Div Publ Hlth Sci, Winston Salem, NC USA. [Pankow, James S.] Univ Minnesota, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Raitakari, Olli T.] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, FIN-20520 Turku, Finland. [Raitakari, Olli T.] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Riserus, Ulf] Uppsala Univ, Dept Publ Hlth & Caring Sci Clin Nutr & Metab, Uppsala, Sweden. [Zillikens, M. Carola; Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Hassanali, Neelam; Groves, Christopher J.; McCarthy, Mark I.; Prokopenko, Inga] Univ Oxford, Oxford Ctr Diabet Endocrinol & Metab, Oxford, England. [Liu, Yongmei] Wake Forest Sch Med, Dept Epidemiol & Prevent, Div Publ Hlth Sci, Winston Salem, NC USA. [Mozaffarian, Dariush] Harvard Univ, Sch Publ Hlth, Dept Epidemiol & Nutr, Boston, MA 02115 USA. [Mozaffarian, Dariush] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Cardiovasc Med, Boston, MA 02115 USA. [Syvanen, Ann-Christine] Uppsala Univ, Mol Med & Sci Life Lab, Dept Med Sci, Uppsala, Sweden. [Viikari, Jorma] Univ Turku, Dept Med, Turku, Finland. [Viikari, Jorma] Turku Univ Hosp, FIN-20520 Turku, Finland. [Lind, Lars] Uppsala Univ, Dept Med Sci, Uppsala, Sweden. [McCarthy, Mark I.] Churchill Hosp, Oxford NIHR Biomed Res Ctr, Oxford OX3 7LJ, England. [Mikkila, Vera] Univ Helsinki, Dept Food & Environm Sci, Helsinki, Finland. [Mukamal, Kenneth] Beth Israel Deaconess Med Ctr, Div Gen Med & Primary Care, Boston, MA 02215 USA. [Cupples, L. Adrienne] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere, Finland. [Kahonen, Mika] Univ Tampere, FIN-33101 Tampere, Finland. [Kao, W. H. Linda] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Prokopenko, Inga] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford, England. [Rotter, Jerome I.] Cedars Sinai Med Ctr, Inst Med Genet, Los Angeles, CA 90048 USA. [Siscovick, David S.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Meigs, James B.] Massachusetts Gen Hosp, Div Gen Med, Clin Epidemiol Unit, Boston, MA 02114 USA. [Meigs, James B.] Massachusetts Gen Hosp, Div Gen Med, Diabet Res Unit, Boston, MA 02114 USA. [Nettleton, Jennifer A.] Univ Texas Hlth Sci Ctr Houston, Sch Publ Hlth, Div Epidemiol Human Genet & Environm Sci, Houston, TX USA. RP McKeown, NM (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu RI Prokopenko, Inga/H-3241-2014; Sonestedt, Emily/I-3814-2016; OI Prokopenko, Inga/0000-0003-1624-7457; Sonestedt, Emily/0000-0002-0747-4562; Cupples, L. Adrienne/0000-0003-0273-7965; Kritchevsky, Stephen/0000-0003-3336-6781; Franks, Paul/0000-0002-0520-7604 FU Pfizer Nutrition FX The full author list and affiliations are included in Supplemental Table 4 in the "Online Supporting Material" link in the online posting of the article and from the same link in the online table of contents at http://jn.nutrition.org. O.H.F. is the recipient of a grant from Pfizer Nutrition to establish a center for research on aging (ErasmusAGE). All other authors declared no conflicts of interest NR 40 TC 23 Z9 23 U1 1 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 2013 VL 143 IS 3 BP 345 EP 353 DI 10.3945/jn.112.172049 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 093EC UT WOS:000315173500014 PM 23343670 ER PT J AU Zheng, JS Arnett, DK Parnell, LD Lee, YC Ma, YY Smith, CE Richardson, K Li, D Borecki, IB Ordovas, JM Tucker, KL Lai, CQ AF Zheng, Ju-Sheng Arnett, Donna K. Parnell, Laurence D. Lee, Yu-Chi Ma, Yiyi Smith, Caren E. Richardson, Kris Li, Duo Borecki, Ingrid B. Ordovas, Jose M. Tucker, Katherine L. Lai, Chao-Qiang TI Genetic Variants at PSMD3 Interact with Dietary Fat and Carbohydrate to Modulate Insulin Resistance SO JOURNAL OF NUTRITION LA English DT Article ID FOOD FREQUENCY QUESTIONNAIRE; GENOME-WIDE ASSOCIATION; PUERTO-RICAN HEALTH; BLOOD-CELL COUNT; FISH-OIL; DIABETES-MELLITUS; GENOTYPE DATA; SENSITIVITY; ADULTS; OMEGA-3-FATTY-ACIDS AB PSMD3 encodes subunit 3 of the 26S proteasome, which is involved in regulating insulin signal transduction, and dietary factors could potentially regulate the function of this gene. We aimed to investigate the associations of PSMD3 variants with glucose-related traits and the interactions of those variants with dietary fat and carbohydrate for glucose-related traits in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study and to replicate the findings in the Boston Puerto Rican Health Study (BPRHS). Ten single nucleotide polymorphisms (SNPs) were selected, covering 90% the genetic variations in or near PSMD3. Minor allele (C) carriers of rs4065321 had higher homeostasis model assessment of insulin resistance (HOMA-IR) than noncarriers in males of both the GOLDN (P = 0.022) and BPRHS (P = 0.036). Minor allele (T) carriers of rs709592 had significantly higher HOMA-IR (P = 0.032) than C homozygotes in the GOLDN, whereas the T allele carriers of rs709592 tended to have higher HOMA-IR (P = 0.08) than C homozygotes in the BPRHS. In the GOLDN, there was an interaction between rs709592 and dietary carbohydrate on HOMA-IR (P = 0.049). Subjects carrying the T allele of rs709592 had higher HOMA-IR compared only with noncarriers with low carbohydrate intake (<= 49.1% energy; P = 0.004). SNPs rs4065321 and rs709592 both significantly interacted with dietary MUFAs and carbohydrate on glucose concentrations in the GOLDN. Our study suggests that PSMD3 variants are associated with insulin resistance in populations of different ancestries and that these relationships may also be modified by dietary factors. J. Nutr. 143: 354-361, 2013: C1 [Zheng, Ju-Sheng; Li, Duo] Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310003, Zhejiang, Peoples R China. [Zheng, Ju-Sheng; Parnell, Laurence D.; Lee, Yu-Chi; Ma, Yiyi; Smith, Caren E.; Richardson, Kris; Ordovas, Jose M.; Lai, Chao-Qiang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Zheng, Ju-Sheng; Li, Duo] APCNS Ctr Nutr & Food Safety, Hangzhou, Zhejiang, Peoples R China. [Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. RP Li, D (reprint author), Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310003, Zhejiang, Peoples R China. EM duoli@zju.edu.cn; chaoqiang.lai@ars.usda.gov OI Zheng, Ju-Sheng/0000-0001-6560-4890 FU China Scholarship Council; National Basic Research Program of China (973 Program) [2011CB504002]; National Heart, Lung and Blood Institute [HL54776, HL078885]; USDA Research Service [53-K06-5-10, 58-1950-9-001] FX Supported by the China Scholarship Council, the National Basic Research Program of China (973 Program: 2011CB504002), National Heart, Lung and Blood Institute grant nos. HL54776 and HL078885, and by contracts 53-K06-5-10 and 58-1950-9-001 from the USDA Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 50 TC 5 Z9 6 U1 0 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 2013 VL 143 IS 3 BP 354 EP 361 DI 10.3945/jn.112.168401 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 093EC UT WOS:000315173500015 PM 23303871 ER PT J AU Larsen, NA Nuessly, GS Cherry, RH Glaz, B AF Larsen, N. A. Nuessly, G. S. Cherry, R. H. Glaz, B. TI Varietal susceptibility to the corn wireworm Melanotus communis (Coleoptera: Elateridae) in sugarcane SO JOURNAL OF PEST SCIENCE LA English DT Article DE Elateridae; Wireworms; Sugarcane; Resistance; Histosol ID FLORIDA; LEPIDOPTERA; PHEROMONE; FIELDS; DAMAGE; YIELD AB Wireworms (larval Elateridae) reduce the stand of newly planted sugarcane (complex hybrid of Saccharum spp.) directly by damaging growing points and indirectly by facilitating disease introduction. No research has evaluated resistance or tolerance of sugarcane genotypes grown in Florida to wireworm. Eleven genotypes of sugarcane and a S. spontaneum genotype were subjected to corn wireworm, Melanotus communis (Gyllenhall) (Coleoptera: Elateridae), in greenhouse experiments to evaluate potential host plant resistance. The experiments were designed to measure the effects of wireworms on the first 90 days of growth. Sugarcane stalk sections were planted in trays of soil with and without wireworms in 2010 and 2011. Stand count, dry weight, and percentage of nodes damaged were evaluated. Wireworms reduced stand and dry weight by 40-60 %. Several genotypes were able to produce acceptable stands in wireworm-infested trays by emerging quickly and producing many tillers. CP 88-1762, CP 89-2143, and CP 03-1912 did not suffer statistically significant losses of stand or biomass due to wireworms in either year. Our findings suggest that genotype resistance should be considered as an important component of an integrated program aimed at reducing the use of insecticides to control wireworms in sugarcane. C1 [Larsen, N. A.; Nuessly, G. S.; Cherry, R. H.] Univ Floridas, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. [Glaz, B.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Larsen, NA (reprint author), Univ Floridas, Everglades Res & Educ Ctr, Inst Food & Agr Sci, 3200 Palm Beach Rd, Belle Glade, FL 33430 USA. EM larsnick@ufl.edu NR 27 TC 2 Z9 2 U1 1 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2013 VL 86 IS 1 SI SI BP 91 EP 98 DI 10.1007/s10340-012-0435-0 PG 8 WC Entomology SC Entomology GA 095QS UT WOS:000315350100011 ER PT J AU Holtkamp, DJ Kliebenstein, JB Neumann, EJ Zimmerman, JJ Rotto, HF Yoder, TK Wang, C Yeske, PE Mowrer, CL Haley, CA AF Holtkamp, Derald J. Kliebenstein, James B. Neumann, Eric J. Zimmerman, Jeffrey. J. Rotto, Hans F. Yoder, Tiffany K. Wang, Chong Yeske, Paul E. Mowrer, Christine L. Haley, Charles A. TI Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers SO JOURNAL OF SWINE HEALTH AND PRODUCTION LA English DT Article DE swine; porcine reproductive and respiratory syndrome virus; economics ID SYNDROME PRRS; SWINE HERDS; LOSSES; PIGS; FARM AB Objective: To estimate the current annual economic impact of porcine reproductive and respiratory syndrome virus (PRRSV) on the US swine industry. Materials and methods: Data for the analysis was compiled from the US Department of Agriculture, a survey of swine veterinarians on the incidence and impact of PRRSV, and production records (2005 to 2010) from commercial farms with known PRRSV status. Animal-level economic impact of productivity losses and other costs attributed to PRRSV were estimated using an enterprise budgeting approach and extrapolated to the national level on the basis of the US breeding-herd inventory, number of pigs marketed, and number of pigs imported for growing. Results: The total cost of productivity losses due to PRRSV in the US national breeding and growing-pig herd was estimated at US $664 million annually, an increase from the US $560 million annual cost estimated in 2005. The 2011 study differed most significandy from the 2005 study in the allocation of losses between the breeding and the growing-pig herd. Losses in the breeding herd accounted for 12% of the total cost of PRRSV in the 2005 study, compared to 45% in the current analysis. Implications: Despite over 25 years of experience and research, porcine reproductive and respiratory syndrome remains a costly disease of pigs in the United States. Since 2005, some progress has been made in dealing with the cost of productivity losses due to the disease in the growing pig, but these were offset by greater losses in the breeding herd. C1 [Holtkamp, Derald J.; Zimmerman, Jeffrey. J.; Yoder, Tiffany K.; Wang, Chong; Mowrer, Christine L.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Kliebenstein, James B.] Iowa State Univ, Coll Agr, Dept Econ, Ames, IA USA. [Neumann, Eric J.] Massey Univ, EpiCtr Inst Vet Anim & Biomed Sci, Palmerston North 4442, New Zealand. [Rotto, Hans F.] Innovat Agr Solut, Ames, IA USA. [Yeske, Paul E.] Swine Vet Ctr, St Peter, MN USA. [Haley, Charles A.] USDA, Ctr Epidemiol, Ft Collins, CO USA. [Haley, Charles A.] USDA, Ctr Anim Sci, Ft Collins, CO USA. RP Holtkamp, DJ (reprint author), 2233 Lloyd Vet Med Ctr, Ames, IA 50011 USA. EM holtkamp@iastate.edu OI Wang, Chong/0000-0003-4489-4344 NR 39 TC 108 Z9 110 U1 9 U2 54 PU AMER ASSOC SWINE VETERINARIANS PI PERRY PA 902 1ST AVE, PERRY, IA 50220-1703 USA SN 1537-209X J9 J SWINE HEALTH PROD JI J. Swine. Health Prod. PD MAR-APR PY 2013 VL 21 IS 2 BP 72 EP 84 PG 13 WC Veterinary Sciences SC Veterinary Sciences GA 095FH UT WOS:000315319600005 ER PT J AU Kepler, RM Rehner, SA AF Kepler, R. M. Rehner, S. A. TI Genome-assisted development of nuclear intergenic sequence markers for entomopathogenic fungi of the Metarhizium anisopliae species complex SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE comparative genomics; marker design; multilocus; nuclear intergenic; species delimitation ID PROFILING PHYLOGENETIC INFORMATIVENESS; MAXIMUM-LIKELIHOOD; BEAUVERIA-BASSIANA; MIXED MODELS; CLASSIFICATION; CONCORDANCE; MOSQUITOS; ALIGNMENT; AFRICAN AB Entomopathogenic fungi in the genus Metarhizium are useful for biological control programmes against economically important arthropod pests worldwide. However, understanding the true diversity and ecology of these organisms is hampered by convergent morphologies between species. The application of molecular techniques has enabled greater resolution of species than allowed by morphology alone. In particular, the commonly used biocontrol agent M. anisopliae was found to be a species complex composed of nine species. This prior work was conducted with commonly used markers in fungal phylogenetics (BTUB, RPB1, RPB2 and TEF), which likely under-represent diversity in the M. anisopliae complex. Using sequence data from nuclear genomes of M. acridum and M. robertsii we identified regions of conserved gene synteny and developed primers to amplify intergenic regions of seven loci. Using ex-type and authenticated tissue specimens for species in the M. anisopliae complex, we demonstrate that sequence data derived from intergenic loci is more variable and phylogenetically informative than previously available markers. These new markers will facilitate investigations at or below the species level for the M. anisopliae complex. The method of marker development employed here should be extendable to any group with sufficiently divergent genome data available. C1 [Kepler, R. M.; Rehner, S. A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. RP Rehner, SA (reprint author), ARS, Systemat Mycol & Microbiol Lab, USDA, Bldg 010A, Beltsville, MD 20705 USA. EM Stephen.Rehner@ARS.USDA.GOV NR 41 TC 18 Z9 18 U1 2 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAR PY 2013 VL 13 IS 2 BP 210 EP 217 DI 10.1111/1755-0998.12058 PG 8 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 091EQ UT WOS:000315032600006 PM 23286460 ER PT J AU Hung, CC Garner, CD Slauch, JM Dwyer, ZW Lawhon, SD Frye, JG McClelland, M Ahmer, BMM Altier, C AF Hung, Chien-Che Garner, Cherilyn D. Slauch, James M. Dwyer, Zachary W. Lawhon, Sara D. Frye, Jonathan G. McClelland, Michael Ahmer, Brian M. M. Altier, Craig TI The intestinal fatty acid propionate inhibits Salmonella invasion through the post-translational control of HilD SO MOLECULAR MICROBIOLOGY LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; TYPE-3 SECRETION SYSTEM; ESCHERICHIA-COLI; GENE-EXPRESSION; EPITHELIAL-CELLS; III SECRETION; GASTROINTESTINAL-TRACT; COA SYNTHETASE; IN-VITRO; BACTERIAL CHEMOTAXIS AB To cause disease, Salmonella must invade the intestinal epithelium employing genes encoded within Salmonella Pathogenicity Island 1 (SPI1). We show here that propionate, a fatty acid abundant in the intestine of animals, repressed SPI1 at physiologically relevant concentration and pH, reducing expression of SPI1 transcriptional regulators and consequently decreasing expression and secretion of effector proteins, leading to reduced bacterial penetration of cultured epithelial cells. Essential to repression was hilD, which occupies the apex of the regulatory cascade within SPI1, as loss of only this gene among those of the regulon prevented repression of SPI1 transcription by propionate. Regulation through hilD, however, was achieved through the control of neither transcription nor translation. Instead, growth of Salmonella in propionate significantly reduced the stability of HilD. Extending protein half-life using a Lon protease mutant demonstrated that protein stability itself did not dictate the effects of propionate and suggested modification of HilD with subsequent degradation as the means of action. Furthermore, repression was significantly lessened in a mutant unable to produce propionyl-CoA, while further metabolism of propionyl-CoA appeared not to be required. These results suggest a mechanism of control of Salmonella virulence in which HilD is post-translationally modified using the high-energy intermediate propionyl-CoA. C1 [Hung, Chien-Che; Garner, Cherilyn D.; Dwyer, Zachary W.; Altier, Craig] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. [Slauch, James M.] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA. [Slauch, James M.] Univ Illinois, Coll Med, Urbana, IL 61801 USA. [Lawhon, Sara D.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Frye, Jonathan G.] Agr Res Serv, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Res Ctr, Athens, GA 30605 USA. [McClelland, Michael] Univ Calif Irvine, Dept Pathol & Lab Med, Irvine, CA 92617 USA. [Ahmer, Brian M. M.] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA. [Ahmer, Brian M. M.] Ohio State Univ, Ctr Microbial Interface Biol, Columbus, OH 43210 USA. RP Altier, C (reprint author), Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. EM altier@cornell.edu RI Frye, Jonathan/I-6382-2013; Lawhon, Sara/G-5147-2011; Ahmer, Brian/F-2228-2010; OI Frye, Jonathan/0000-0002-8500-3395; Lawhon, Sara/0000-0001-9154-8909; Ahmer, Brian/0000-0002-4267-7322; Slauch, James/0000-0003-4634-9702; McClelland, Michael/0000-0003-1788-9347 FU USDA Cooperative State Research, Education and Extension Service [2005-35201-16270]; NIH [AI039557, AI052237, AI073971, AI075093, AI077645, AI083646]; USDA [2009-0357930127, 2011-67017-30127]; Binational Agricultural Research and Development Fund; CDMRP BCRP [W81XWH-08-1-0720] FX We gratefully acknowledge the assistance of Jingwen Zhang in developing the protein stability assays. This project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, award number 2005-35201-16270. MM was supported in part by NIH grants AI039557, AI052237, AI073971, AI075093, AI077645, AI083646, USDA grants 2009-0357930127 and 2011-67017-30127, the Binational Agricultural Research and Development Fund, and CDMRP BCRP W81XWH-08-1-0720. NR 80 TC 23 Z9 23 U1 0 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-382X EI 1365-2958 J9 MOL MICROBIOL JI Mol. Microbiol. PD MAR PY 2013 VL 87 IS 5 BP 1045 EP 1060 DI 10.1111/mmi.12149 PG 16 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 096JH UT WOS:000315399500008 PM 23289537 ER PT J AU Wang, XJ Zhu, XP Tooley, P Zhang, XG AF Wang, Xiuju Zhu, Xiaoping Tooley, Paul Zhang, Xiuguo TI Cloning and functional analysis of three genes encoding polygalacturonase-inhibiting proteins from Capsicum annuum and transgenic CaPGIP1 in tobacco in relation to increased resistance to two fungal pathogens SO PLANT MOLECULAR BIOLOGY LA English DT Article DE Capsicum annuum; Polygalacturonase-inhibitingproteins; Endopolygalacturonases; Biotic stress; Abiotic stress; Transient expression; Disease resistance ID PHASEOLUS-VULGARIS L; LEUCINE-RICH REPEAT; PLANT-CELL WALLS; SIGNAL-TRANSDUCTION PATHWAYS; HOST-SPECIFIC TOXINS; BOTRYTIS-CINEREA; ABSCISIC-ACID; DISEASE RESISTANCE; SALICYLIC-ACID; DEFENSE RESPONSES AB Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall glycoproteins that can inhibit fungal endopolygalacturonases (PGs). The PGIPs directly reduce the aggressive potential of PGs. Here, we isolated and functionally characterized three members of the pepper (Capsicum annuum) PGIP gene family. Each was up-regulated at a different time following stimulation of the pepper leaves by Phytophthora capcisi and abiotic stresses including salicylic acid, methyl jasmonate, abscisic acid, wounding and cold treatment. Purified recombinant proteins individually inhibited activity of PGs produced by Alternaria alternata and Colletotrichum nicotianae, respectively, and virus-induced gene silencing in pepper conferred enhanced susceptibility to P. capsici. Because three PGIP genes acted similarily in conferring resistance to infection by P. capsici, and because individually purified proteins showed consistent inhibition against PG activity of both pathogens, CaPGIP1 was selected for manipulating transgenic tobacco. The crude proteins from transgenic tobacco exhibited distinct enhanced resistance to PG activity of both fungi. Moreover, the transgenic tobacco showed effective resistance to infection and a significant reduction in the number of infection sites, number of lesions and average size of lesions in the leaves. All results suggest that CaPGIPs may be involved in plant defense response and play an important role in a plant's resistance to disease. C1 [Wang, Xiuju; Zhu, Xiaoping; Zhang, Xiuguo] Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China. [Tooley, Paul] ARS, Foreign Dis Weed Sci Res Unit, USDA, Ft Detrick, MD 21702 USA. RP Zhang, XG (reprint author), Shandong Agr Univ, Dept Plant Pathol, 61 Daizong St, Tai An 271018, Shandong, Peoples R China. EM sdau613@163.com; zhxg@sdau.edu.cn FU The Project Graveness Gene of China [2009ZX08009-050B]; National Natural Science Foundation of China, NSFC [30871620] FX This research was supported by The Project Graveness Gene of China (2009ZX08009-050B). National Natural Science Foundation of China, NSFC (30871620). We very thank Gary J. Samuels for assisting in revising this manuscript. NR 122 TC 11 Z9 15 U1 2 U2 65 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4412 EI 1573-5028 J9 PLANT MOL BIOL JI Plant Mol.Biol. PD MAR PY 2013 VL 81 IS 4-5 BP 379 EP 400 DI 10.1007/s11103-013-0007-6 PG 22 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 096CP UT WOS:000315381900005 PM 23334855 ER PT J AU Misra, D Booth, SL Tolstykh, I Felson, DT Nevitt, MC Lewis, CE Torner, J Neogi, T AF Misra, Devyani Booth, Sarah L. Tolstykh, Irina Felson, David T. Nevitt, Michael C. Lewis, Cora E. Torner, James Neogi, Tuhina TI Vitamin K Deficiency Is Associated with Incident Knee Osteoarthritis SO AMERICAN JOURNAL OF MEDICINE LA English DT Article DE Incident knee osteoarthritis; MRI cartilage abnormalities; Vitamin K ID MATRIX GLA PROTEIN; BIOCHEMICAL MEASURES; HAND OSTEOARTHRITIS; CARTILAGE LOSS; WOMEN; RISK; MEN; DIETARY; MINERALIZATION; PHYLLOQUINONE AB BACKGROUND: Osteoarthritis is the most common form of arthritis, with knee osteoarthritis being the leading cause of lower extremity disability among older adults in the US. There are no treatments available to prevent the structural pathology of osteoarthritis. Because of vitamin K's role in regulating skeletal mineralization, it has potential to be a preventative option for osteoarthritis. We therefore examined the relation of vitamin K to new-onset radiographic knee osteoarthritis and early osteoarthritis changes on magnetic resonance imaging (MRI). METHODS: Subjects from the Multicenter Osteoarthritis (MOST) Study had knee radiographs and MRI scans obtained at baseline and 30 months later, and plasma phylloquinone (vitamin K) measured at baseline. We examined the relationship of subclinical vitamin K deficiency to incident radiographic knee osteoarthritis and MRI-based cartilage lesions and osteophytes, respectively, using log binomial regression with generalized estimating equations, adjusting for potential confounders. RESULTS: Among 1180 participants (62% women, mean age 62 +/- 8 years, mean body mass index 30.1 +/- 5.1 kg/m(2)), subclinical vitamin K deficiency was associated with incident radiographic knee osteoarthritis (risk ratio [RR] 1.56; 95% confidence interval [CI], 1.08-2.25) and cartilage lesions (RR 2.39; 95% CI, 1.05-5.40) compared with no deficiency, but not with osteophytes (RR 2.35; 95% CI, 0.54-10.13). Subclinically vitamin K-deficient subjects were more likely to develop osteoarthritis in one or both knees than neither knee (RR 1.33; 95% CI, 1.01-1.75 and RR 2.12; 95% CI, 1.06-4.24, respectively). CONCLUSION: In the first such longitudinal study, subclinical vitamin K deficiency was associated with increased risk of developing radiographic knee osteoarthritis and MRI-based cartilage lesions. Further study of vitamin K is warranted given its therapeutic/prophylactic potential for osteoarthritis. (C) 2013 Elsevier Inc. All rights reserved. The American Journal of Medicine (2013) 126, 243-248 C1 [Misra, Devyani; Felson, David T.; Neogi, Tuhina] Boston Univ, Sch Med, Boston, MA 02215 USA. [Booth, Sarah L.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. [Tolstykh, Irina; Nevitt, Michael C.] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Lewis, Cora E.] Univ Alabama Birmingham, Birmingham, AL USA. [Torner, James] Univ Iowa, Iowa City, IA USA. RP Misra, D (reprint author), Clin Epidemiol Unit, 650 Albany St,Suite X-200, Boston, MA 02118 USA. EM devyani.misra@BMC.org OI Misra, Devyani/0000-0003-2881-7920; Felson, David/0000-0002-2668-2447; Neogi, Tuhina/0000-0002-9515-1711 FU Arthritis Foundation Clinical; NIAMS [K23 AR055127]; Boston Claude D. Pepper Older Americans Independence Center Research Career Development Core; NIA [U01-AG18820, U01-AG19069, U01-AG18947, U01-AG18832]; US Department of Agriculture, Agricultural Research Service [58-1950-7-707]; National Institutes of Health [AG14759, HL696272]; NIH [AR47785] FX D. Misra is supported by the Arthritis Foundation Clinical to Research Transition Award. T. Neogi is supported by NIAMS K23 AR055127, the Arthritis Foundation Arthritis Investigator Award, and Boston Claude D. Pepper Older Americans Independence Center Research Career Development Core. Support for the Multicenter Osteoarthritis (MOST) Study is by NIA U01-AG18820 (Felson, PI), U01-AG19069 (Nevitt, PI), U01-AG18947 (Lewis, PI), U01-AG18832 (Torner, PI). This work also was supported by the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707, and the National Institutes of Health (AG14759, HL696272), NIH AR47785. NR 38 TC 12 Z9 12 U1 2 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9343 J9 AM J MED JI Am. J. Med. PD MAR PY 2013 VL 126 IS 3 BP 243 EP 248 DI 10.1016/j.amjmed.2012.10.011 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA 088YM UT WOS:000314873800024 PM 23410565 ER PT J AU Irwin, P Reed, S Brewster, J Nguyen, L He, YP AF Irwin, Peter Reed, Sue Brewster, Jeffrey Ly Nguyen He, Yiping TI Non-stochastic sampling error in quantal analyses for Campylobacter species on poultry products SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Food-borne pathogens; Campylobacter; Real-time PCR; qPCR; MPN ID REAL-TIME PCR; O157 IMMUNOMAGNETIC BEADS; ESCHERICHIA-COLI O157H7; PROBABLE-NUMBER-PCR; LISTERIA-MONOCYTOGENES; SALMONELLA SPP.; NONTARGET MICROORGANISMS; VIBRIO-PARAHAEMOLYTICUS; ENVIRONMENTAL-SAMPLES; ANTI-SALMONELLA AB Using primers and fluorescent probes specific for the most common food-borne Campylobacter species (Campylobacter jejuni and Campylobacter coli), we developed a multiplex, most probable number (MPN) assay using quantitative PCR (qPCR) as the determinant for binomial detection: i.e., number of p positive pathogen growth responses out of n = 6 observations each of 4 mL (V) per dilution. Working with media washes of thrice frozen-thawed chicken pieces which had been spiked with known levels of C. jejuni and C. coli, we found that about 20 % of the experiments had a significant amount of error in the form of either greater than 25 % MPN calculation error (Delta epsilon) and/or a low apparent recovery rate (R less than 1 = MPN observed A center dot CFU spiked). Assuming such errors were exacerbated by an excessively small n, we examined computer-generated MPN enumeration data from the standpoint of stochastic sampling error (Delta) and found that such binomial-based assays behaved identically to Poisson-based methods (e.g., counting data) except that fewer technical replicates (n) appeared to be required for the same number of cells per test volume (mu). This result implies that the qPCR detection-based MPN protocol discussed herein should accurately enumerate a test population with a mu a parts per thousand yenaEuro parts per thousand 1 using n = 6 observations per dilution. For our protocol, this equates to a parts per thousand yenaEuro parts per thousand 8 cells per 400-500 g of sampled product. Based on this analysis, the error rate we saw in spiked experiments (where mu > > 1) implied a non-stochastic source. In other experiments we present evidence that this source was, at least in part, related to the cell concentration step (i.e., centrifugation). We also demonstrate that the error rate lessened (from similar to 38 % to similar to 13 %) at lower Campylobacter levels (mu a parts per thousand currency signaEuro parts per thousand 40) as would most likely exist in nature. Using this protocol, we were able to quantify 14 to 1,226 MPN per 450 g of naturally contaminated chicken for skinless pieces and 11 to 244 MPN per 450 g for wings, breasts, legs, and thighs (skin on) whereupon about 50 % of the 29 samples tested negative for both species. Four of these chicken wash samples did have substantially lower Campylobacter levels (1 to 6 MPN per 450 g) which might be better enumerated using a larger n. However, we established that the limit of quantification of this protocol diminishes for n > 6 because one is ever more diluting the sample, or lessening V, to achieve the requisite n. C1 [Irwin, Peter; Reed, Sue; Brewster, Jeffrey; Ly Nguyen; He, Yiping] ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Wyndmoor, PA 19038 USA. RP Irwin, P (reprint author), ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM peter.irwin@ars.usda.gov; yiping.he@ars.usda.gov NR 46 TC 2 Z9 2 U1 1 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD MAR PY 2013 VL 405 IS 7 BP 2353 EP 2369 DI 10.1007/s00216-012-6659-2 PG 17 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 091IW UT WOS:000315043800026 PM 23380949 ER PT J AU Pieper, JR Laugero, KD AF Pieper, Joy Rickman Laugero, Kevin D. TI Preschool children with lower executive function may be more vulnerable to emotional-based eating in the absence of hunger SO APPETITE LA English DT Article DE Cognitive function; Childhood obesity; Impulsivity; Disinhibited eating; Delay of gratification; Children's gambling task ID BEHAVIOR QUESTIONNAIRE; YOUNG-CHILDREN; FOOD-INTAKE; COGNITIVE FUNCTION; CHILDHOOD OBESITY; PEDIATRIC OBESITY; EFFORTFUL CONTROL; FEEDING PRACTICES; DECISION-MAKING; SELF-REGULATION AB Decreased executive function (EF) has been linked to unhealthy eating behaviors and obesity in older children and adults, however little is known about this relationship in young children. One possible reason for this association is that individuals with degraded EF are more vulnerable to emotional-based overeating. Emotional eating may thus be more likely to occur in persons with lower self-control or ability to regulate emotions. A pilot project in a research-based preschool was conducted to examine the relationships between executive function, emotional arousal and eating in the absence of hunger (EAH) in 3-6 year-old children. Executive function was measured through child-completed tasks, parent questionnaires, and standardized teacher reports. Emotional arousal was measured via skin conductance. Children who had lower cognitive development scores as indicated by teacher reports had higher EAH. Increased emotional arousal was associated with increased EAH, but only in a subgroup of children who had a lower capacity for emotional regulation as suggested by lower delay of gratification scores, lower effortful control (parent questionnaire), and overall lower teacher-reported cognitive development. Further studies are necessary to determine whether interventions to improve executive function and emotional regulation in young children may also have the benefit of improving eating behaviors and decreasing risk of obesity in the long run. Published by Elsevier Ltd. C1 [Pieper, Joy Rickman; Laugero, Kevin D.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Laugero, Kevin D.] USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Laugero, KD (reprint author), Univ Calif Davis, Dept Nutr, 1 Shields Ave, Davis, CA 95616 USA. EM Kevin.Laugero@ars.usda.gov NR 56 TC 16 Z9 17 U1 6 U2 105 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 J9 APPETITE JI Appetite PD MAR 1 PY 2013 VL 62 BP 103 EP 109 DI 10.1016/j.appet.2012.11.020 PG 7 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA 091SO UT WOS:000315070300013 PM 23211377 ER PT J AU Font, JC Hernandez-Quevedo, C McDonald, JT Variyam, JN AF Font, Joan Costa Hernandez-Quevedo, Cristina McDonald, James Ted Variyam, Jayachandran N. TI Understanding Healthy Lifestyles: The Role of Choice and the Environment SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Editorial Material ID EXCESS WEIGHT; OBESITY; GAIN C1 [Font, Joan Costa] London Sch Econ, London, England. [Font, Joan Costa] Harvard Univ, Cambridge, MA 02138 USA. [Hernandez-Quevedo, Cristina] LSE Hlth, European Observ Hlth, London, England. [McDonald, James Ted] Univ New Brunswick, Fredericton, NB E3B 5A3, Canada. [Variyam, Jayachandran N.] ERS, USDA, Washington, DC USA. RP Font, JC (reprint author), London Sch Econ, London, England. NR 17 TC 0 Z9 0 U1 0 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2013 VL 35 IS 1 SI SI BP 1 EP 6 DI 10.1093/aepp/pps051 PG 6 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 092YN UT WOS:000315159000001 ER PT J AU Kalenkoski, CM Hamrick, KS AF Kalenkoski, Charlene M. Hamrick, Karen S. TI How Does Time Poverty Affect Behavior? A Look at Eating and Physical Activity SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Time use; Discretionary time; Time poverty; Time-poor; American Time Use Survey; Eating and Health Module; Energy balance; Exercise; Eating patterns; Fast food; Active travel; I12; J10; I30 ID SEDENTARY BEHAVIORS; US ADULTS; OBESITY; ASSOCIATION; POPULATION; NUTRITION; EXERCISE AB This paper uses data on daily activities from the American Time Use Survey and the associated Eating Health Module to analyze the relationships between time poverty and specific energy-balance behaviors. The authors estimate a simultaneous model to jointly analyze the relationships between time poverty and the probability of a fast food purchase, the number of eating and drinking occurrences, minutes spent engaging in sports and exercise, and the probability of engaging in active travel (walking or cycling). Time-poor individuals were found to have different eating and physical activity patterns than non-time-poor individuals; those who were time-poor were less likely to purchase fast food and also less likely to engage in active travel. C1 [Kalenkoski, Charlene M.] Ohio Univ, Dept Econ, Athens, OH 45701 USA. [Hamrick, Karen S.] ERS, USDA, Washington, DC USA. RP Hamrick, KS (reprint author), ERS, USDA, Washington, DC USA. EM khamrick@ers.usda.gov NR 45 TC 5 Z9 7 U1 3 U2 37 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2013 VL 35 IS 1 SI SI BP 89 EP 105 DI 10.1093/aepp/pps034 PG 17 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 092YN UT WOS:000315159000006 ER PT J AU Schroeter, C Anders, S Carlson, A AF Schroeter, Christiane Anders, Sven Carlson, Andrea TI The Economics of Health and Vitamin Consumption SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Vitamins; Endogeneity; Instrumental variables; Healthy Eating Index2005; I12; D12; C26 ID NO ADDITIONAL DATA; DIETARY-SUPPLEMENTS; CONSTRUCTING INSTRUMENTS; BEHAVIORAL-FACTORS; MEASUREMENT ERROR; FOOD; QUALITY; CONSUMERS; DEMAND; INFORMATION AB We estimate the impact of vitamin supplement intake, lifestyle, health indicators, food culture, and demographics on diet quality outcomes as measured by the Healthy Eating Index2005 (HEI). Our data consists of U.S. adults who participated in the 20032004 National Health and Nutrition Examination Survey. Alternative instrumental variable estimators explicitly address issues of endogeneity and complex sample design. Our empirical analysis demonstrates that diet quality is strongly interrelated with food culture. We suggest that vitamin consumption serves as another marker for healthy eating. This finding emphasizes the need to employ economic modeling when developing public policy to reduce obesity. C1 [Schroeter, Christiane] Calif Polytech State Univ San Luis Obispo, San Luis Obispo, CA 93407 USA. [Anders, Sven] Univ Alberta, Edmonton, AB T6G 2M7, Canada. [Carlson, Andrea] ERS, USDA, Washington, DC USA. RP Schroeter, C (reprint author), Calif Polytech State Univ San Luis Obispo, San Luis Obispo, CA 93407 USA. EM cschroet@calpoly.edu NR 75 TC 6 Z9 6 U1 3 U2 24 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2013 VL 35 IS 1 SI SI BP 125 EP 149 DI 10.1093/aepp/pps040 PG 25 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 092YN UT WOS:000315159000008 ER PT J AU Andrews, M Bhatta, R Ploeg, MV AF Andrews, Margaret Bhatta, Rhea Ploeg, Michele Ver TI An Alternative to Developing Stores in Food Deserts: Can Changes in SNAP Benefits Make a Difference? SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Food access; Supplemental Nutrition Assistance Program (SNAP); Food deserts; Food stamps; I14; I38; Q18 ID WELFARE-REFORM; STAMP PROGRAM; ASSET LIMITS; ACCESS; PRICES; DIET; US AB In the search for policies to reduce the effects of limited food access, little consideration has been given to how economic incentives could be used to make it easier for low-income families to access existing healthy food retailers. Using county-level, administrative data on redemption of Supplemental Nutrition Assistance Program (SNAP) benefits by store type from May 2007 to May 2010, this paper investigates aggregate responses of SNAP participants to economic and policy changes. Results show that SNAP benefit increases, in general, are associated with a greater percentage of redemptions at superstores. However, other circumstances associated with the large increase in benefits enacted in April 2009 as a part of the stimulus bill reverse the positive effect. Estimates are stable across a number of specifications that also control for gas prices, store-type density, local unemployment and state policies. Results suggest that economic incentives deserve further consideration as an alternative to store development in food desert communities. C1 [Andrews, Margaret; Ploeg, Michele Ver] ERS, Food Econ Div, USDA, Washington, DC USA. [Bhatta, Rhea] Lehigh Univ, Bethlehem, PA USA. RP Ploeg, MV (reprint author), ERS, Food Econ Div, USDA, Washington, DC USA. EM sverploeg@ers.usda.gov NR 27 TC 2 Z9 2 U1 3 U2 44 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2013 VL 35 IS 1 SI SI BP 150 EP 170 DI 10.1093/aepp/pps042 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 092YN UT WOS:000315159000009 ER PT J AU Zhang, XQ Chen, M Ma, XL Zhao, XF Wang, JX Shao, HL Song, QS Stanley, D AF Zhang, Xiaoqian Chen, Ming Ma, Xinlei Zhao, Xiaofan Wang, Jinxing Shao, Honglian Song, Qisheng Stanley, David TI SUPPRESSION OF AcMNPV REPLICATION BY ADF AND THYMOSIN PROTEIN UP-REGULATION IN A NEW TESTIS CELL LINE, Ha-shl-t SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE AcMNPV; thymosin; adf; F-actin; testis cell line ID NUCLEAR POLYHEDROSIS-VIRUS; ACTIN CYTOSKELETON; FILAMENTOUS ACTIN; VIRAL-INFECTION; BACULOVIRUS; NUCLEOPOLYHEDROVIRUS; NUCLEOCAPSIDS; ESTABLISHMENT; INSECTS; BINDING AB Host cytoskeletons facilitate the entry, replication, and egress of viruses because cytoskeletons are essential for viral survival. One mechanism of resisting viral infections involves regulating cytoskeletal polymerization/depolymerization. However, the molecular mechanisms of regulating these changes in cytoskeleton to suppress viral replication remain unclear. We established a cell line (named Ha-shl-t) from the pupal testis of Helicoverpa armigera (Lepidoptera: Noctuidae). The new testis cell line suppresses Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) replication via disassembly of cytoskeleton. Up-regulation of thymosin (actin disassembling factor) and adf (actin depolymerizing factor) reduces F-actin. Silencing thymosin or adf or treating cells with the F-actin stabilizer phalloidin led to increased AcMNPV replication, while treating cells with an F-actin assembly inhibitor cytochalasin B decreased viral replication. We infer that Ha-shl-t cells utilize F-actin depolymerization to suppress AcMNPV replication by up-regulating thymosin and adf. We propose Ha-shl-t as a model system for investigating cytoskeletal regulation in antiviral action and testicular biology generally. C1 [Zhang, Xiaoqian; Chen, Ming; Ma, Xinlei; Zhao, Xiaofan; Wang, Jinxing; Shao, Honglian] Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Jinan 250100, Peoples R China. [Song, Qisheng; Stanley, David] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO USA. RP Shao, HL (reprint author), Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China. EM shaohl@sdu.edu.cn FU National Natural Science Foundation of China [30770281, 31071977]; Shandong Provincial Natural Science Foundation, China [Y2007D51] FX Grant sponsor: National Natural Science Foundation of China; Grant numbers: 30770281; 31071977; Grant sponsor: Shandong Provincial Natural Science Foundation, China; Grant number: Y2007D51. NR 27 TC 2 Z9 3 U1 0 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD MAR PY 2013 VL 82 IS 3 BP 158 EP 171 DI 10.1002/arch.21082 PG 14 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 092EN UT WOS:000315102100005 PM 23315790 ER PT J AU Homan, HJ Stahl, RS Linz, GM AF Homan, H. Jeffrey Stahl, Randal S. Linz, George M. TI Comparison of two models for estimating mortality from baitings with Compound DRC-1339 Concentrate avicide SO CROP PROTECTION LA English DT Article DE Avicide; Blackbirds; DRC-1339; Icteridae; Modeling; Mortality ID EUROPEAN STARLINGS; BROWN RICE; BIRDS; METABOLISM; CARCASSES; FLIGHT; CORN AB In the U.S., DRC-1339 baitings for blackbirds (Icteridae) are generally done under the pesticide label, Compound DRC-1339 Concentrate - Staging Areas. DRC-1339 is a slow-acting avicide and gives the birds enough time to leave the baiting sites. Carcass searches and other forms of onsite counts are ineffective. Instead, linear models (LM) are used. The LM are based on esophageal analyses of several blackbird species collected while feeding at staging area bait sites. Biases and large variances can occur with this type of sampling. As an alternative to the LM, we developed a semi-mechanistic model (SM) that combined mechanistic modeling of environmental and biophysical processes with statistical modeling of DRC-1339 toxicities, avian physical and physiological traits, and foraging behavior. We used simulated baiting scenarios in Missouri and Louisiana to quantify and compare mortality between the LM and SM. The SM accounted for meteorological and regional effects on feeding rates, and we ran the SM scenarios for both mild and inclement weather conditions during January, a month when DRC-1339 baitings frequently occur. Mortality was calculated for males and females of three blackbird species. We used brown rice as the delivery substrate in a mix consisting of 11.34 kg untreated and 0.45 kg 2% DRC-1339 treated rice (1:25 dilution ratio). Compared to the LM, estimates by the SM ranged from 5% higher for male common grackles [Quiscalus quiscula L] to 59% lower for male brown-headed cowbirds [Molothrus ater Boddaert]. On average, the SM was 29% lower ((x) over bar = 8635, SE = 274.6) than the LM ((x) over bar = 12,131, SE = 1530.8, P < 0.001). Mortality estimates by the SM were 21% lower (<(x)over bar> = 7630, SE = 235.2, n = 12) under inclement than mild conditions ((x) over bar = 9641, SE = 2763, P < 0.001). Latitudinal difference between the states did not affect mortality estimates produced by the SM (P > 0.65). Unlike the LM, the SM used avian physiological and behavioral responses to environmental and meteorological conditions based on individual characteristics of the modeled blackbird species. It represents a scientifically rigorous and broad-scale approach that can be applied at all staging area baitings regardless of region or time-of-year. The SM will produce much lower mortality estimates compared to the LM when brown-headed cowbirds are the major species using staging area sites. Published by Elsevier Ltd. C1 [Homan, H. Jeffrey; Linz, George M.] Natl Wildlife Res Ctr, USDA, Anim Plant Hlth Inspect Serv, Wildlife Serv, Bismarck, ND 58501 USA. [Stahl, Randal S.] Natl Wildlife Res Ctr, USDA, Anim Plant Hlth Inspect Serv, Wildlife Serv, Bismarck, ND 58501 USA. RP Homan, HJ (reprint author), Natl Wildlife Res Ctr, USDA, Anim Plant Hlth Inspect Serv, Wildlife Serv, 2110 Miriam Circle, Bismarck, ND 58501 USA. EM jeffrey.h.homan@aphis.usda.gov NR 40 TC 1 Z9 1 U1 1 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD MAR PY 2013 VL 45 BP 71 EP 75 DI 10.1016/j.cropro.2012.11.011 PG 5 WC Agronomy SC Agriculture GA 090XF UT WOS:000315012600011 ER PT J AU Wang, JC Jia, Y Wen, JW Liu, WP Liu, XM Li, L Jiang, ZY Zhang, JH Guo, XL Ren, JP AF Wang, J. C. Jia, Y. Wen, J. W. Liu, W. P. Liu, X. M. Li, L. Jiang, Z. Y. Zhang, J. H. Guo, X. L. Ren, J. P. TI Identification of rice blast resistance genes using international monogenic differentials SO CROP PROTECTION LA English DT Article DE Rice; Magnaporthe oyrzae; Resistance genes; Monogenic lines; Pathogenicity assay; Breeding strategy ID NEAR-ISOGENIC LINES; DNA MARKERS; PI-TA; PATHOTYPES; VARIETIES; GRISEA; ORYZAE; CHINA AB Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases of rice that severely affects crop production in the Jilin Province, Northeast China, where temperate japonica rice is primarily grown. In the following study, 44 representative local blast isolates were inoculated onto international monogenic differentials carrying 24 major blast resistance genes - Pia, Pib, Pii, Pik, Pik-h, Pik-m, Pik-p, Pik-s, Pish, Pit, Pita, Pita-2, Piz, Piz-t, Pi1, Piz-5, Pi3, Pi5(t), Pi7(t), Pi9, Pil2(t), Pi11(t), Pi19, and Pi20 - and the susceptible recurrent parent, Lijiangxintuanheigu (LTH), under greenhouse conditions. The percentage of virulent reactions of monogenic lines to the 44 isolates was found ranging from 8.3% to 79.2%. LTH was susceptible to all 44 isolates. All 24 monogenic differential lines were resistant to at least 4 isolates of M. oryzae, and the frequency of resistant reactions of the monogenic lines carrying Pi9, Pi19, Piz, Piz-5, Piz-t, Pi12(t), Pi5(t), and Pik-h were 94.2%, 84.1%, 81.8%, 81.8%, 79.5%, 72.7%, 68.2%, and 68.2%, respectively. These results suggest that Pi9, Pi19, Piz, Piz-5, Piz-t, Pi12(t), Pi5(t) and Pik-h may be important R genes for preventing blast disease. Based on these data, a useful strategy for managing rice blast disease by stacking pyramiding blast R genes against pathogenic M. oryzae isolates in the Jilin Province was proposed. (c) 2013 Elservier Ltd. All rights reserved. C1 [Wang, J. C.; Wen, J. W.; Liu, W. P.; Liu, X. M.; Li, L.; Jiang, Z. Y.; Zhang, J. H.; Guo, X. L.; Ren, J. P.] Jilin Acad Agr Sci, Inst Plant Protect, Changchun 130033, Jilin, Peoples R China. [Jia, Y.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Wang, JC (reprint author), Jilin Acad Agr Sci, Inst Plant Protect, Changchun 130033, Jilin, Peoples R China. EM wangjichun1972@yahoo.com FU S&T development fund of the Jilin province government [20100578]; State Administration of Foreign Experts Affairs, the P. R. of China [CG20112200017] FX The authors thank IRRI,and Cailin Lei (ICS-CAAS) for providing monogenic line rice seeds, Ellen McWhirter for proof reading the revised manuscript. The research was partly supported by grants from the S&T development fund of the Jilin province government (20100578) and a Training Project from the State Administration of Foreign Experts Affairs, the P. R. of China (CG20112200017). The USDA is an equal opportunity provider and employer. NR 34 TC 7 Z9 7 U1 3 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD MAR PY 2013 VL 45 BP 109 EP 116 DI 10.1016/j.cropro.2012.11.020 PG 8 WC Agronomy SC Agriculture GA 090XF UT WOS:000315012600017 ER PT J AU Perez-Diaz, IM AF Perez-Diaz, Ilenys M. TI Putative and Unique Gene Sequence Utilization for the Design of Species Specific Probes as Modeled by Lactobacillus plantarum SO CURRENT MICROBIOLOGY LA English DT Article ID IDENTIFICATION AB The concept of utilizing putative and unique gene sequences for the design of species specific probes was tested. The abundance profile of assigned functions within the Lactobacillus plantarum genome was used for the identification of the putative and unique gene sequence, csh. The targeted gene (csh) was used as the template for PCR amplification and construction of a non-radioactive DIG labeled probe. The csh derived probe aided in the preliminary and rapid identification of L. plantarum from mixed cultures by colony hybridization. The method described here for the rapid identification of L. plantarum can also be applied for the rapid detection of other bacteria if a unique gene sequence can be identified from its complete genome sequence. C1 N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. RP Perez-Diaz, IM (reprint author), N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM Ilenys.Perez-Diaz@ars.usda.gov NR 14 TC 2 Z9 2 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 J9 CURR MICROBIOL JI Curr. Microbiol. PD MAR PY 2013 VL 66 IS 3 BP 266 EP 270 DI 10.1007/s00284-012-0265-6 PG 5 WC Microbiology SC Microbiology GA 079PF UT WOS:000314182400009 PM 23183932 ER PT J AU Murdock, JN Shields, FD Lizotte, RE AF Murdock, Justin N. Shields, F. Douglas, Jr. Lizotte, Richard E., Jr. TI Periphyton responses to nutrient and atrazine mixtures introduced through agricultural runoff SO ECOTOXICOLOGY LA English DT Article DE Algae; Agricultural runoff; Herbicide; Metabolism; Pulse; Wetland ID FRESH-WATER ALGAE; COMPARATIVE SENSITIVITY; DIFFERENTIAL TOXICITY; SMALL STREAM; PHYTOPLANKTON; COMMUNITIES; PESTICIDES; DEGRADATION; METABOLISM; PHOTOSYNTHESIS AB Agricultural runoff often contains pollutants with antagonistic impacts. The individual influence of nutrients and atrazine on periphyton has been extensively studied, but their impact when introduced together and with multiple agricultural pollutants is less clear. We simulated a field-scale runoff pulse into a riverine wetland that mimicked pollutant composition typical of field runoff of the Mississippi River Alluvial Plain. Periphyton biomass and functional responses were measured for 2 weeks along a 500 m section. Additionally, laboratory chamber assays were used to identify potential periphyton changes due to nutrients, atrazine, and their interactions. Generally, nutrients stimulated, and atrazine reduced chlorophyll a (Chl a) in chambers. In the wetland, nutrient and atrazine relationships with periphyton were weaker, and when found, were often opposite of trends in chambers. Total nitrogen (TN) was inversely related to Chl a, and total phosphorus was inversely related to respiration (R) rates. Atrazine (10-20 mu g L-1 in the wetland) had a positive relationship with ash-free dry mass (AFDM), and weakened the relationship between TN and AFDM. Wetland periphyton biomass was better correlated to total suspended solids than nutrients or atrazine. Periphyton function was resilient as periphyton gross primary production (GPP)/R ratios were not strongly impacted by runoff. However, whole-system GPP and R decreased over the 2-week period, suggesting that although periphyton metabolism recovered quickly, whole-system metabolism took longer to recover. The individual and combined impacts of nutrients and atrazine in complex pollutant mixtures can vary substantially from their influence when introduced separately, and non-linear impacts can occur with distance downstream of the pollutant introduction point. C1 [Murdock, Justin N.; Shields, F. Douglas, Jr.; Lizotte, Richard E., Jr.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Murdock, Justin N.] Tennessee Technol Univ, Dept Biol, Cookeville, TN 38501 USA. RP Murdock, JN (reprint author), Tennessee Technol Univ, Dept Biol, POB 5063, Cookeville, TN 38501 USA. EM jnmurdock@tntech.edu NR 66 TC 13 Z9 14 U1 3 U2 79 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0963-9292 J9 ECOTOXICOLOGY JI Ecotoxicology PD MAR PY 2013 VL 22 IS 2 BP 215 EP 230 DI 10.1007/s10646-012-1018-9 PG 16 WC Ecology; Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 089GF UT WOS:000314898100002 PM 23179409 ER PT J AU Miklas, PN Porter, LD Kelly, JD Myers, JR AF Miklas, Phillip N. Porter, Lyndon D. Kelly, James D. Myers, James R. TI Characterization of white mold disease avoidance in common bean SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Dry bean; Lodging; Microclimate; Phaseolus vulgaris; Sclerotinia sclerotiorum; Snap bean ID IMPROVING PHYSIOLOGICAL RESISTANCE; ROOT ARCHITECTURE TRAITS; PHASEOLUS-VULGARIS L.; SCLEROTINIA-SCLEROTIORUM; AGRONOMIC TRAITS; PLANT ARCHITECTURE; CANOPY STRUCTURE; QTL ANALYSIS; REGISTRATION; IDENTIFICATION AB White mold, caused by Sclerotinia sclerotiorum, is a devastating fungal disease of common bean (Phaseolus vulgaris L.) worldwide. Physiological resistance and disease avoidance conferred by plant architecture-related traits contribute to white mold field resistance. Our objective was to further examine white mold disease avoidance in common bean. A comparative map composed of 79 quantitative trait loci (QTL) for white mold resistance (27), disease avoidance traits (36) and root traits (16) was generated. Thirteen white mold resistance QTL, six with strong and seven with weak associations with disease avoidance traits, were observed. Root length and lodging QTL co-located in three regions. Canopy porosity and height, and lodging were highly correlated with disease severity score in field screening trials conducted from 2000 to 2011. Resistance to lodging was extremely important for reducing disease severity in both dry and snap bean (r = 0.61 across 11 trials). Avoidance traits were less effective in reducing disease severity in trials with heavy disease pressure. Dry bean lines with physiological resistance in combination with disease avoidance traits did not require fungicide application to protect yield potential under moderate and heavy disease pressure. Given the complexity of disease resistance as evidenced by the comparative QTL map, marker-assisted breeding for disease avoidance is not recommended at this time. Instead, selecting for resistance to white mold in the field, in combination with high yield potential and acceptable maturity, is the recommended strategy for improving both disease avoidance and physiological resistance to white mold in cultivars with commercially acceptable agronomic traits. C1 [Miklas, Phillip N.; Porter, Lyndon D.] USDA ARS, Vegetable & Forage Crop Res Unit, Prosser, WA 99350 USA. [Kelly, James D.] Dept Plant Soil & Microbial Sci, E Lansing, MI USA. [Myers, James R.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Miklas, PN (reprint author), USDA ARS, Vegetable & Forage Crop Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM phil.miklas@ars.usda.gov FU National Sclerotinia Initiative FX The authors would like to thank the National Sclerotinia Initiative for funding part of this research (http://www.ars.usda.gov/Research/docs.htm?docid=20317), and appreciate technical support from Jennifer Trapp, and the many field technicians and graduate students who managed the field trials and collected data. NR 63 TC 27 Z9 27 U1 4 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD MAR PY 2013 VL 135 IS 3 SI SI BP 525 EP 543 DI 10.1007/s10658-012-0153-8 PG 19 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 087FZ UT WOS:000314748000007 ER PT J AU Vero, S Garmendia, G Gonzalez, MB Bentancur, O Wisniewski, M AF Vero, Silvana Garmendia, Gabriela Belen Gonzalez, M. Bentancur, Oscar Wisniewski, Michael TI Evaluation of yeasts obtained from Antarctic soil samples as biocontrol agents for the management of postharvest diseases of apple (Malusxdomestica) SO FEMS YEAST RESEARCH LA English DT Article DE biocontrol; Antarctic yeasts; postharvest ID SACCHAROMYCES-CEREVISIAE; BOTRYTIS-CINEREA; CITRUS-FRUIT; BLUE MOLD; CANDIDA; SIDEROPHORES; RESISTANCE; ARGENTINA; PATHOGENS; URUGUAY AB Psychrotrophic yeasts were isolated from Antarctic soils, selected based on their ability to grow in apple juice at low temperatures, and were evaluated as potential biocontrol agents for the management of postharvest diseases of apple during cold storage. Among the species recovered, an isolate of Leucosporidium scottii, designated At17, was identified as a good biocontrol agent for blue and gray mold of two apple cultivars. The selected isolate produced soluble and volatile antifungal substances that were inhibitory to apple pathogens. Siderophore production was also demonstrated, but it did not appear to play a role in pathogen inhibition. The selected yeast had the capacity to form a biofilm when grown in apple juice, which is considered an important attribute of postharvest antagonists to successfully colonize wounds and intact fruit surfaces. At17 was resistant to commonly used postharvest fungicides, so application of a combination of low-dose fungicide along with the biocontrol agent could be used as an integrated management practice. C1 [Vero, Silvana; Garmendia, Gabriela; Belen Gonzalez, M.] UdelaR, Fac Quim, Catedra Microbiol, Dept Biociencias, Montevideo 11800, Uruguay. [Bentancur, Oscar] UdelaR, Fac Agron, Dept Biometria Estadist & Computac, Montevideo 11800, Uruguay. [Wisniewski, Michael] USDA ARS, Kearneysville, WV USA. RP Vero, S (reprint author), UdelaR, Fac Quim, Catedra Microbiol, Dept Biociencias, Gral Flores 2124, Montevideo 11800, Uruguay. EM svero@fq.edu.uy FU Instituto Antartico Uruguayo (IAU) FX We are very grateful to Instituto Antartico Uruguayo (IAU) who supported this work. We also want to thank Dr Silvia Batista for providing Antarctic soil samples. NR 43 TC 14 Z9 15 U1 1 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1567-1356 J9 FEMS YEAST RES JI FEMS Yeast Res. PD MAR PY 2013 VL 13 IS 2 BP 189 EP 199 DI 10.1111/1567-1364.12021 PG 11 WC Biotechnology & Applied Microbiology; Microbiology; Mycology SC Biotechnology & Applied Microbiology; Microbiology; Mycology GA 089ND UT WOS:000314916400005 PM 23136855 ER PT J AU Lee, SM Yoo, J Inglett, GE Lee, S AF Lee, Seung Mi Yoo, Jiyoung Inglett, George E. Lee, Suyong TI Particle Size Fractionation of High-Amylose Rice (Goami 2) Flour as an Oil Barrier in a Batter-Coated Fried System SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Particle size; High-amylose rice flour; Oil uptake; Frying; Rheology ID PHYSICOCHEMICAL PROPERTIES; STARCH GELATINIZATION; TORTILLA CHIPS; FRYING BATTERS; QUALITY; RHEOLOGY AB The particle size effects of high-amylose rice (Goami 2) flour on quality attributes of frying batters were characterized in terms of physicochemical, rheological, and oil-resisting properties. High-amylose rice flours were fractionated into four fractions (70, 198, 256, and 415 mu m) of which morphology was also analyzed by scanning electron microscopy. Rice flour with smaller particle size exhibited a higher degree of starch gelatinization, giving rise to increased pasting parameters. When the rice flours were incorporated into frying batters, higher steady shear viscosity was observed in the batters with finer rice flour, which could be well characterized by the power law model. In addition, the dynamic viscoelastic properties of the batters were enhanced by the use of rice flour with smaller particle size, which also caused an increase in batter pickup. When subjected to deep fat frying, the batters with finer rice flour exhibited reduced moisture loss. Furthermore, the oil uptake was found to have a positive correlation with the particle size of rice flour (R (2) = 0.88), even showing the reduction of oil uptake by 15%. It could be synergistically attributed to the formation of outer starch granular layers, high batter viscosity/pickup, and reduced moisture loss by finer rice flour. C1 [Lee, Seung Mi; Yoo, Jiyoung; Lee, Suyong] Sejong Univ, Dept Food Sci & Technol, Seoul 143747, South Korea. [Lee, Seung Mi; Yoo, Jiyoung; Lee, Suyong] Sejong Univ, Carbohydrate Bioprod Res Ctr, Seoul 143747, South Korea. [Inglett, George E.] ARS, Cereal Prod & Food Sci Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Lee, S (reprint author), Sejong Univ, Dept Food Sci & Technol, 98 Gunja Dong, Seoul 143747, South Korea. EM suyonglee@sejong.ac.kr FU National Research Foundation of Korea; Korea government (MEST) [2010-0008483] FX This work was supported by the National Research Foundation of Korea grant funded by the Korea government (MEST) (No. 2010-0008483) NR 32 TC 7 Z9 8 U1 2 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD MAR PY 2013 VL 6 IS 3 BP 726 EP 733 DI 10.1007/s11947-011-0721-5 PG 8 WC Food Science & Technology SC Food Science & Technology GA 087NQ UT WOS:000314768800010 ER PT J AU Khir, R Pan, ZL Atungulu, GG Thompson, JF Shao, DY AF Khir, Ragab Pan, Zhongli Atungulu, Griffiths G. Thompson, James F. Shao, Dongyan TI Size and Moisture Distribution Characteristics of Walnuts and Their Components SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Moisture content; Distribution; Size characteristics; Walnut; Ethephon; Hull; Shell; Kernel ID NUT KERNEL RECOVERY; ROUGH RICE; PHYSICAL-PROPERTIES; QUALITY; MODELS; SYSTEM AB The objective of this study was to determine the size characteristics and moisture content (MC) distributions of individual walnuts and their components, including hulls, shells, and kernels under different harvest conditions. Measurements were carried out for three walnut varieties, Tulare, Howard, and Chandler cultivated in California, USA. The samples for each variety were collected from the harvester at the first and second harvest of nuts treated with and without ethephon. The nuts were sorted into two categories as with hulls and without hulls before conducting dimension and MC measurements. The results showed that there was a wide range of size distribution for nuts with and without hulls and a huge variability in moisture content among individual nuts at harvest. The average MC of nuts with hulls was much higher than that of nuts without hulls for all tested varieties. The nuts with hulls had an average moisture content of 32.99% compared to 13.86% for nuts without hulls. Also, the shell moisture content was much higher than kernel moisture content. On average, the differences in moisture content between shell and kernel was 11.56% for nuts with hulls and 6.45% for nuts without hulls. There was no significant deference in hull MC between the first and second harvest for the studied varieties. Based on the regression analysis, it was observed that strong relationships exist between the MC of shells and kernels. The obtained results provide information for designing and developing new handling and processing equipments, especially for increased drying capacity, reduced energy use, and obtaining high-quality walnut products. C1 [Khir, Ragab; Pan, Zhongli; Atungulu, Griffiths G.; Thompson, James F.; Shao, Dongyan] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Khir, Ragab] Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia, Egypt. RP Pan, ZL (reprint author), ARS, Proc Foods Res Unit, USDA, WRRC, 800 Buchanan St, Albany, CA 94710 USA. EM Zhongli.Pan@ars.usda.gov FU California Walnut Board FX The authors thank the California Walnut Board for its financial support and Cilker Orchards for their support with walnut samples. The research was conducted at the Western Regional Research Center of USDA-ARS and Department of Biological and Agricultural Engineering, University of California, Davis, USA. NR 34 TC 2 Z9 3 U1 3 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD MAR PY 2013 VL 6 IS 3 BP 771 EP 782 DI 10.1007/s11947-011-0717-1 PG 12 WC Food Science & Technology SC Food Science & Technology GA 087NQ UT WOS:000314768800015 ER PT J AU Dolanc, CR Thorne, JH Safford, HD AF Dolanc, Christopher R. Thorne, James H. Safford, Hugh D. TI Widespread shifts in the demographic structure of subalpine forests in the Sierra Nevada, California, 1934 to 2007 SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE California; climate change; composition shifts; demographic structure; forest; resampling; Sierra Nevada; subalpine; VTM; Wieslander ID YOSEMITE-NATIONAL-PARK; WESTERN UNITED-STATES; RECENT CLIMATE-CHANGE; MOUNTAIN PINE-BEETLE; TREE MORTALITY-RATES; WHITEBARK-PINE; PLANT-DISTRIBUTION; VEGETATION CHANGE; BRISTLECONE-PINE; CONIFER FORESTS AB Aim Many climate-linked vegetation models predict major contraction of subalpine forests within the next 100 years, which would require a relatively rapid replacement of high-elevation species by lower-elevation species over large portions of subalpine forest. We tested this prediction by comparing empirical data from a historic data set with data collected from re-sampled sites from 200709. Location Central Sierra Nevada, CA, USA, 23003400m elevation. Methods We re-sampled 139 undisturbed historical vegetation plots across 5500km2 originally sampled from 192934 in the subalpine zone of the Sierra Nevada, and compared historical with current forest structure and composition. We compared historic and modern climatic conditions using two high-elevation climate stations nearby. Results Subalpine forests experienced a net increase in tree stem density of 30.4%, including a 63.3% increase in small trees. Six of eight tree species showed statistically significant increases in small tree density, including species with distributions at both the upper and lower boundaries of subalpine. Increases in small tree density were partly offset by a 20% decrease in large trees. These shifts were significant throughout the landscape of our study area. Modern stand composition was indistinguishable from historical composition. Daily minimum temperature (+1.2 degrees C) and precipitation (+1548%) both increased during the same period. Main conclusions Warming temperatures plus steady to increasing precipitation have led to less stressful conditions for recruitment and survival of small trees, and are probably contributing to increased mortality of large trees. Tree abundance and composition in the subalpine has not changed in the direction predicted by vegetation models linked to future climate scenarios. Our results underline the fundamental role that moisture balance plays in structuring mediterranean-zone montane forests. Future shifts in vegetation composition and structure from these regions are likely to depend on interactions between water balance and disturbance factors like fire, insects and disease. C1 [Dolanc, Christopher R.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Dolanc, Christopher R.] Univ Calif Davis, Ecol Grad Grp, Davis, CA 95616 USA. [Thorne, James H.; Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Safford, Hugh D.] US Forest Serv, USDA, Vallejo, CA 94592 USA. RP Dolanc, CR (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM crdolanc@ucdavis.edu FU National Science Foundation [0819493]; California Energy Commission PIER Program [CEC PIR-08-006]; USDA Forest Service Pacific Southwest Region Ecology Program; Tahoe Conservancy; Ernest Hill Foundation of UC-Davis; Davis Botanical Society; Northern California Botanists; California Native Plant Society FX We thank M. Barbour, M. Schwartz, C. Millar, R. Westfall and two anonymous referees for their helpful comments. A. Holguin provided help with figure preparation and field work. C. Delong, C. Calloway, E. Peck, K. Farrell, N. Le, D. Showers, C. Peters and D. Ingrasia helped with field work. Funding came from the National Science Foundation award no. 0819493, the California Energy Commission PIER Program CEC PIR-08-006, the USDA Forest Service Pacific Southwest Region Ecology Program, The Tahoe Conservancy, The Ernest Hill Foundation of UC-Davis, The Davis Botanical Society, Northern California Botanists, and the California Native Plant Society. NR 70 TC 32 Z9 35 U1 3 U2 121 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD MAR PY 2013 VL 22 IS 3 BP 264 EP 276 DI 10.1111/j.1466-8238.2011.00748.x PG 13 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA 092AA UT WOS:000315090000002 ER PT J AU Rickard, BJ Okrent, AM Alston, JM AF Rickard, Bradley J. Okrent, Abigail M. Alston, Julian M. TI HOW HAVE AGRICULTURAL POLICIES INFLUENCED CALORIC CONSUMPTION IN THE UNITED STATES? SO HEALTH ECONOMICS LA English DT Article DE agricultural policy; caloric intake; consumer support; food consumption; obesity; simulation model ID BODY-WEIGHT; OBESITY; SYSTEM; QUANTIFICATION; PREVALENCE; OVERWEIGHT; SUBSIDIES; DEMAND; TRENDS; PRICE AB Many commentators have speculated that agricultural policies have contributed to increased obesity rates in the United States, yet such claims are often made without any analysis of the complex links between real-world farm commodity support programs, prices and consumption of foods, and caloric intake. This article carefully studies the effects of US agricultural policies on prices and quantities of 10 agricultural commodities and nine food categories in the United States over time. Using a detailed multimarket model, we simulate the counterfactual removal of measures of support applied to US agricultural commodities in 1992, 1997, and 2002 and quantify the effects on US food consumption and caloric intake. To parameterize the simulations, we calculate three alternative measures of consumer support (the implicit consumer subsidy from policies that support producers) for the 10 agricultural commodities using information about government expenditures on agricultural commodities from various sources. Our results indicate thatholding all other policies constantremoving US subsidies on grains and oilseeds in the three periods would have caused caloric consumption to decrease minimally whereas removal of all US agricultural policies (including barriers against imports of sugar and dairy products) would have caused total caloric intake to increase. Our results also indicate that the influence of agricultural policies on caloric intake has diminished over time. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Rickard, Bradley J.] Cornell Univ, Charles H Dyson Sch Appl Econ & Management, Ithaca, NY 14853 USA. [Okrent, Abigail M.] Econ Res Serv, USDA, Washington, DC USA. [Alston, Julian M.] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA 95616 USA. RP Rickard, BJ (reprint author), Cornell Univ, Sch Appl Econ & Management, Ithaca, NY 14853 USA. EM bjr83@cornell.edu FU National Research Initiative from the USDA National Institute for Food and Agriculture [2006-55215-16720]; USDA Economic Research Service [58-3000-8-013]; Giannini Foundation of Agricultural Economics FX Primary financial support for the work in this article was provided by the National Research Initiative grant 2006-55215-16720 from the USDA National Institute for Food and Agriculture. Additional support was provided by the USDA Economic Research Service through a cooperative research project (agreement 58-3000-8-013) and by amini-grant from the Giannini Foundation of Agricultural Economics. The views expressed here are those of the authors and not necessarily those of the US Department of Agriculture. The authors gratefully acknowledge valuable input provided by two anonymous reviewers and by Kym Anderson, Joanna Parks, and Christiane Schroeter. NR 76 TC 12 Z9 12 U1 1 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1057-9230 J9 HEALTH ECON JI Health Econ. PD MAR PY 2013 VL 22 IS 3 BP 316 EP 339 DI 10.1002/hec.2799 PG 24 WC Economics; Health Care Sciences & Services; Health Policy & Services SC Business & Economics; Health Care Sciences & Services GA 087HO UT WOS:000314752300005 PM 22331635 ER PT J AU Grohman, K Cameron, R Kim, Y Widmer, W Luzio, G AF Grohman, Karel Cameron, Randall Kim, Yang Widmer, Wilbur Luzio, Gary TI Extraction and recovery of pectic fragments from citrus processing waste for coproduction with ethanol SO JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY LA English DT Article DE pectin; citrus; biofuels; galacturonic acid; processing waste ID DILUTE-ACID HYDROLYSIS; ORANGE PEEL; SIMULTANEOUS SACCHARIFICATION; ESCHERICHIA-COLI; CELL-WALLS; FERMENTATION; SUGARS; SIZE; METHYLESTERASE; PRETREATMENT AB BACKGROUND: Approximately 0.7 1 x 106 dry tons of citrus processing waste (CPW) are produced annually in the United States. CPW is sold as animal feed but often the financial return does not exceed the production cost. Polysaccharides comprise 40% of the total dry matter of which pectin is the major component. CPW was steam treated to extract pectin fragments (PFs) as a value added coproduct prior to fermentation of other sugars for biofuels production. RESULTS: PFs were extracted in high yields, along with polymeric arabinans, galactans and arabinogalactans. The extracted polysaccharides ranged in size from small oligomers to polymers of approximate to 700 000 g mol1. Acidified treatments led to greater fragmentation of water soluble polysaccharides, but did not enhance fragmentation of pectins to small oligomers (> 30 mer). Methylesterified PFs, arabinans and galactans were recovered by ethanol precipitation while demethylesterified PFs were recovered and purified by precipitation with dilute HCl. CONCLUSION: Steam treatment of CPW provides for rapid, efficient fragmentation of protopectin into highly methylesterified PFs that could be recovered by precipitation. The steaming process for preparation of PFs is environmentally friendly. No toxic chemicals are introduced and the remaining CPW can be used in fermentations to produce ethanol and other compounds. (c) 2012 Society of Chemical Industry C1 [Grohman, Karel] Renewable Spirits LLC, Delray Beach, FL USA. [Cameron, Randall; Kim, Yang; Widmer, Wilbur; Luzio, Gary] ARS, USDA, Citrus & Subtrop Prod Res Unit, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Cameron, R (reprint author), ARS, USDA, Citrus & Subtrop Prod Res Unit, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM Randall.cameron@ars.usda.gov FU USDA-CSREES-SBIR Grant FX 2010 USDA-CSREES-SBIR Grant, Phase 1, 2010 NR 30 TC 12 Z9 12 U1 4 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-2575 J9 J CHEM TECHNOL BIOT JI J. Chem. Technol. Biotechnol. PD MAR PY 2013 VL 88 IS 3 BP 395 EP 407 DI 10.1002/jctb.3859 PG 13 WC Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical SC Biotechnology & Applied Microbiology; Chemistry; Engineering GA 092LN UT WOS:000315123400009 ER PT J AU Ueno, M Shen, WJ Patel, S Greenberg, AS Azhar, S Kraemer, FB AF Ueno, Masami Shen, Wen-Jun Patel, Shailja Greenberg, Andrew S. Azhar, Salman Kraemer, Fredric B. TI Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress SO JOURNAL OF LIPID RESEARCH LA English DT Article DE adipose; inflammation; lipid droplet; nuclear translocation; protein-protein interaction ID HORMONE-SENSITIVE LIPASE; WHITE ADIPOSE-TISSUE; LIPID DROPLETS; DEFICIENT MICE; ALDOSE REDUCTASE; BINDING PROTEIN; OSMOTIC-STRESS; LIVING CELLS; TRANSCRIPTION; FSP27 AB Fat-specific protein 27 (FSP27), a member of the cell death-inducing DNA fragmentation factor alpha-like effector (Cide) family, is highly expressed in adipose tissues and is a lipid droplet (LD)-associated protein that induces the accumulation of LDs. Using a yeast two-hybrid system to examine potential interactions of FSP27 with other proteins, a direct interaction with the N-terminal region of nuclear factor of activated T cells 5 (NFAT5) was identified. NFAT5 is a transcription factor that induces osmoprotective and inflammatory genes after its translocation to the nucleus. The interaction between FSP27 and NFAT5 was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation. Using immunocytochemistry, NFAT5 is detected in the cytoplasm and in the nucleus under isotonic conditions; however, overexpression of FSP27 inhibited the hypertonic-induced nuclear translocation of NFAT5. Consistent with the suppression of NFAT5 nuclear translocation, in cells transfected with a reporter construct containing the NFAT5 response element from the monocyte chemoattractant protein 1 (MCP1) promoter, FSP27 overexpression repressed hypertonic-induced luciferase activity and the expression of NFAT5 target genes. Knockdown of FSP27 in differentiated 3T3-L1 adipocytes increased the NFAT5-mediated rise in MCP1. These results suggest that FSP27 not only modulates LD homeostasis but also modulates the response to osmotic stress via a physical interaction with NFAT5 at the LD surface.-Ueno, M., W.-J. Shen, S. Patel, A. S. Greenberg, S. Azhar, and F. B. Kraemer. Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress. J. Lipid Res. 2013. 54: 734-743. C1 [Ueno, Masami; Shen, Wen-Jun; Patel, Shailja; Azhar, Salman; Kraemer, Fredric B.] Vet Adm Palo Alto Hlth Care Syst, Palo Alto, CA USA. [Ueno, Masami; Shen, Wen-Jun; Patel, Shailja; Kraemer, Fredric B.] Stanford Univ, Div Endocrinol Gerontol & Metab, Stanford, CA 94305 USA. [Greenberg, Andrew S.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Medford, MA USA. RP Ueno, M (reprint author), Vet Adm Palo Alto Hlth Care Syst, Palo Alto, CA USA. EM fbk@stanford.edu FU Department of Veterans Affairs (Office of Research and Development, Medical Research Service); American Diabetes Association [7-08-RA-57, 7-12-BS-100]; USDA Agricultural Research Service [58-1950-7-707]; National Institutes of Health [DK082574, 1RC2ES01871, R24DK0867669, R01AG028098, 1R01HL92473, 2R01HL033881] FX This work was supported by the Department of Veterans Affairs (Office of Research and Development, Medical Research Service), by funding from the American Diabetes Association (7-08-RA-57 and 7-12-BS-100) and the USDA Agricultural Research Service (58-1950-7-707), and by National Institutes of Health grants DK082574, 1RC2ES01871, R24DK0867669, R01AG028098, 1R01HL92473, and 2R01HL033881. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health or other granting agencies. NR 44 TC 9 Z9 15 U1 0 U2 8 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 J9 J LIPID RES JI J. Lipid Res. PD MAR PY 2013 VL 54 IS 3 BP 734 EP 743 DI 10.1194/jlr.M033365 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 088ZM UT WOS:000314877000016 PM 23233732 ER PT J AU de Campos, A Tonoli, GHD Marconcini, JM Mattoso, LHC Klamczynski, A Gregorski, KS Wood, D Williams, T Chiou, BS Imam, SH AF de Campos, Adriana Tonoli, Gustavo H. D. Marconcini, Jose M. Mattoso, Luiz H. C. Klamczynski, Artur Gregorski, Kay S. Wood, Delilah Williams, Tina Chiou, Bor-Sen Imam, Syed H. TI TPS/PCL Composite Reinforced with Treated Sisal Fibers: Property, Biodegradation and Water-Absorption SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Starch; Thermoplastic; Water absorption; Contact angle; Extrusion; Biopolymers ID MECHANICAL-PROPERTIES; THERMOPLASTIC STARCH; PLASTICIZED STARCH; MULTIPHASE SYSTEMS; CELLULOSE; BLENDS; POLY(EPSILON-CAPROLACTONE); POLYCAPROLACTONE; CRYSTALLIZATION; WETTABILITY AB Sisal fibers bleached with sodium-hydroxide followed by hydrogen peroxide treatment were incorporated in a thermoplastic starch/epsilon-polycaprolactone (TPS/PCL) blend via extrusion processing. These samples with smooth and homogenous surfaces were examined for their property, biodegradability and water absorption. Scanning electron microscopy revealed that the fibers were well dispersed in the matrix. In addition, it was found that the fibers and matrices interacted strongly. Blends with 20 % (dry weight-basis) fiber content showed some fiber agglomeration. Whereas blends with 10 % fibers showed increased crystallinity and lower water absorption capacity. The CO2 evolution study showed that the thermoplastic starch samples without any additives had the highest rate and extent of degradation whereas the neat PCL samples had the lowest degradation rate. Addition of fiber to the TPS/PCL blend exhibited the degradation rates and extents that were somewhere in between the pure TPS and neat PCL. This work demonstrates that TPS/PCL composites reinforced with bleached sisal has superior structural characteristics and water resistance and thus, can be used as polymeric engineering composites for different applications. C1 [de Campos, Adriana; Marconcini, Jose M.; Mattoso, Luiz H. C.] Embrapa Instrumentacao, LNNA, BR-13560970 Sao Carlos, SP, Brazil. [Tonoli, Gustavo H. D.] Univ Fed Lavras, Dept Forest Sci, Lavras, MG, Brazil. [Klamczynski, Artur; Gregorski, Kay S.; Wood, Delilah; Williams, Tina; Chiou, Bor-Sen; Imam, Syed H.] USDA ARS, Bioprod Chem & Engn Res Unit, WRRC, Albany, CA 94710 USA. RP Imam, SH (reprint author), USDA ARS, Bioprod Chem & Engn Res Unit, WRRC, Albany, CA 94710 USA. EM syed.imam@ars.usda.gov RI tonoli, gustavo/I-4821-2013; Marconcini, Jose/D-9702-2013; Campos, Adriana/F-7070-2012; Mattoso, Luiz H C/D-2794-2016 OI Campos, Adriana/0000-0002-1499-2659; Mattoso, Luiz H C/0000-0001-7586-1014 NR 35 TC 13 Z9 13 U1 1 U2 61 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 J9 J POLYM ENVIRON JI J. Polym. Environ. PD MAR PY 2013 VL 21 IS 1 BP 1 EP 7 DI 10.1007/s10924-012-0512-8 PG 7 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA 089CO UT WOS:000314886900001 ER PT J AU Dickinson, N Morel, JL Shaw, RK Wessolek, G AF Dickinson, Nicholas Morel, Jean-Louis Shaw, Richard K. Wessolek, Gerd TI IUSS SUITMA 6 International Symposium 2011 SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Editorial Material C1 [Dickinson, Nicholas] Lincoln Univ, Dept Ecol, Canterbury 7647, New Zealand. [Morel, Jean-Louis] Univ Lorraine, INRA, F-54618 Vandoeuvre Les Nancy, France. [Shaw, Richard K.] USDA NRCS, Somerset, NJ 08873 USA. [Wessolek, Gerd] Tech Univ Berlin, Inst Ecol, Fac 4, D-10587 Berlin, Germany. RP Morel, JL (reprint author), Univ Lorraine, INRA, 2 Ave Foret de Haye,TSA 40602, F-54618 Vandoeuvre Les Nancy, France. EM Jean-Louis.Morel@univ-lorraine.fr OI Morel, Jean Louis/0000-0002-5720-1482 NR 1 TC 0 Z9 0 U1 2 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 J9 J SOIL SEDIMENT JI J. Soils Sediments PD MAR PY 2013 VL 13 IS 3 BP 489 EP 490 DI 10.1007/s11368-013-0656-8 PG 2 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA 091GR UT WOS:000315037900001 ER PT J AU Pega, J Bucafusco, D Di Giacomo, S Schammas, JM Malacari, D Capozzo, AV Arzt, J Perez-Beascoechea, C Maradei, E Rodriguez, LL Borca, MV Perez-Filgueira, M AF Pega, J. Bucafusco, D. Di Giacomo, S. Schammas, J. M. Malacari, D. Capozzo, A. V. Arzt, J. Perez-Beascoechea, C. Maradei, E. Rodriguez, L. L. Borca, M. V. Perez-Filgueira, M. TI Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle SO JOURNAL OF VIROLOGY LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; ANTIBODY-RESPONSE; PHARYNGEAL FLUID; LIVE-VIRUS; PATHOGENESIS; EPIDEMIC; FMDV; QUANTIFICATION; LOCALIZATION; IMMUNIZATION AB Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure. C1 [Pega, J.; Bucafusco, D.; Di Giacomo, S.; Schammas, J. M.; Malacari, D.; Capozzo, A. V.; Perez-Filgueira, M.] INTA, CICVyA, Inst Virol, Buenos Aires, DF, Argentina. [Pega, J.; Bucafusco, D.; Capozzo, A. V.; Perez-Filgueira, M.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Arzt, J.; Rodriguez, L. L.; Borca, M. V.] USDA ARS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Perez-Beascoechea, C.; Maradei, E.] Serv Nacl Sanidad & Calidad Agroalimentaria SENAS, Direcc Labs, Buenos Aires, DF, Argentina. RP Perez-Filgueira, M (reprint author), INTA, CICVyA, Inst Virol, Buenos Aires, DF, Argentina. EM mperez@cnia.inta.gov.ar OI Borca, Manuel/0000-0002-0888-1178; Arzt, Jonathan/0000-0002-7517-7893 FU Agricultural Research Service [58-1940-8-111F]; Agencia Nacional de Promocion Cientifica y Tecnologica [PICT26-PAE 37.206]; Instituto Nacional de Tecnologia Agropecuaria FMD Project [AESA 201721] FX This work was funded by Agricultural Research Service collaborative agreement 58-1940-8-111F, the Agencia Nacional de Promocion Cientifica y Tecnologica (PICT26-PAE 37.206), and Instituto Nacional de Tecnologia Agropecuaria FMD Project AESA 201721. NR 36 TC 13 Z9 13 U1 2 U2 12 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD MAR PY 2013 VL 87 IS 5 BP 2489 EP 2495 DI 10.1128/JVI.02879-12 PG 7 WC Virology SC Virology GA 088ZL UT WOS:000314876900011 PM 23255811 ER PT J AU Debboun, M Strickman, D AF Debboun, M. Strickman, D. TI Insect repellents and associated personal protection for a reduction in human disease SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Review DE Individual prevention; insecticide-treated bednet; integrated pest management; malaria; mosquito; mosquito coil; public health; repellent; vector-borne disease ID DEET MOSQUITO REPELLENT; MALARIA CONTROL; AEDES-AEGYPTI; TREATED NETS; BED NETS; PREVENTION; VECTORS; EFFICACY; TRIAL; LEISHMANIASIS AB Personal protection measures against biting arthropods include topical insect repellents, area repellents, insecticide-treated bednets and treated clothing. The literature on the effectiveness of personal protection products against arthropods is mainly limited to studies of prevention of bites, rather than prevention of disease. Tungiasis was successfully controlled by application of topical repellents and scrub typhus was reduced through the use of treated clothing. Successful reduction of leishmaniasis was achieved through the use of topical repellents, treated bednets and treated clothing in individual studies. Malaria has been reduced by the use of insecticide-treated bednets (ITN), certain campaigns involving topical repellents, and the combination of treated bednets and topical repellents. Although area repellents such as mosquito coils are used extensively, their ability to protect humans from vector-transmitted pathogens has not been proven. Taken together, the literature indicates that personal protection measures must be used correctly to be effective. A study that showed successful control of malaria by combining treated bednets and topical repellents suggests that combinations of personal protection measures are likely to be more effective than single methods. Implementation of successful programmes based on personal protection will require a level of cooperation commonly associated with other basic societal functions, such as education and food safety. C1 [Debboun, M.] USA, Dept Prevent Hlth Serv, Acad Hlth Sci, Med Dept Ctr, Ft Sam Houston, TX USA. [Strickman, D.] ARS, Off Natl Programs, USDA, Beltsville, MD USA. RP Strickman, D (reprint author), ARS, USDA, Natl Program Staff, Room 4-2112,5601 Sunnyside Ave, Beltsville, MD 20705 USA. EM daniel.strickman@ars.usda.gov NR 61 TC 28 Z9 29 U1 1 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD MAR PY 2013 VL 27 IS 1 BP 1 EP 9 DI 10.1111/j.1365-2915.2012.01020.x PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 091EU UT WOS:000315033000001 PM 22624654 ER PT J AU Matsumoto, TK Keith, LM Cabos, RYM Suzuki, JY Gonsalves, D Thilmony, R AF Matsumoto, Tracie K. Keith, Lisa M. Cabos, Roxana Y. M. Suzuki, Jon Y. Gonsalves, Dennis Thilmony, Roger TI Screening promoters for Anthurium transformation using transient expression SO PLANT CELL REPORTS LA English DT Article DE Anthurium; Monocot; Promoter; Transformation; GUS ID TRANSGENE EXPRESSION; PLANT TRANSFORMATION; GENE-TRANSFER; HIGH-LEVEL; RESISTANCE AB There are multiple publications on Anthurium transformation, yet a commercial product has not been achieved. This may be due to use of non-optimum promoters here we address this problem. Different promoters and tissue types were evaluated for transient beta-glucuronidase (GUS) expression in Anthurium andraeanum Hort. 'Marian Seefurth' following microprojectile bombardment. Plasmids containing the Ubiquitin 2, Actin 1, Cytochrome C1 from rice, Ubiquitin 1 from maize and 35S promoter from Cauliflower Mosaic Virus fused to a GUS reporter gene were bombarded into in vitro grown anthurium lamina, somatic embryos and roots. The number of GUS foci and the intensity of GUS expression were evaluated for each construct. Ubiquitin promoters from rice and maize resulted in the highest number of expressing cells in all tissues examined. Due to the slow growth of anthurium plants, development of transgenic anthurium plants takes years. This research has rapidly identified multiple promoters that express in various anthurium tissues facilitating the development of transformation vectors for the expression of desirable traits in anthurium plants. C1 [Matsumoto, Tracie K.; Keith, Lisa M.; Cabos, Roxana Y. M.; Suzuki, Jon Y.; Gonsalves, Dennis] ARS, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Thilmony, Roger] ARS, USDA, Western Reg Res Ctr Crop, Improvement & Utilizat Res Unit, Albany, CA 94710 USA. RP Matsumoto, TK (reprint author), ARS, USDA, Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM tracie.matsumoto@ars.usda.gov NR 35 TC 6 Z9 6 U1 5 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0721-7714 J9 PLANT CELL REP JI Plant Cell Reports PD MAR PY 2013 VL 32 IS 3 BP 443 EP 451 DI 10.1007/s00299-012-1376-z PG 9 WC Plant Sciences SC Plant Sciences GA 089KT UT WOS:000314910100011 PM 23283558 ER PT J AU Aver'yanov, AA Zakharenkova, TS Lapikova, VP Pasechnik, TD Gaivoronskaya, LM Baker, CJ AF Aver'yanov, A. A. Zakharenkova, T. S. Lapikova, V. P. Pasechnik, T. D. Gaivoronskaya, L. M. Baker, C. J. TI Exogenous superoxide dismutase may lose its antidotal ability on rice leaves SO RUSSIAN JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Oryza sativa; Magnaporthe grisea; rice blast disease; leaf diffusates; fungitoxicity; superoxide dismutase ID ACTIVE OXYGEN; OXIDATIVE BURST; SALICYLIC-ACID; EXTRACELLULAR CATALASE; ACQUIRED-RESISTANCE; CLAVICEPS-PURPUREA; BOTRYTIS-CINEREA; HOST-DEFENSE; PLANT; PATHOGENICITY AB Leaf diffusates of the resistant rice (Oryza sativa L.) cultivars suppressed spore germination of blast fungus (Magnaporthe grisea (Hebert) Barr). Bovine Cu-Zn superoxide dismutase (SOD) added to the diffusate abolished its toxicity. However, the enzyme added to the inoculum did not affect the toxicity of the diffusate. Even the second SOD portion added to the diffusate was ineffective. As well, the enzyme exposed to leaves could not protect the fungus from artificially-generated superoxide. Presumably, SOD contacting with leaves induced an efflux of compound(s) inhibiting both portions of the enzyme. Evidence was obtained suggesting that neither enzymatic protein nor zinc of coenzyme but copper might be the inducer. A comparison of rice leaves and callus culture together with the effects of exogenous salicylic acid suggests that this compound may be the inhibitor liberating from leaves. It is not excluded that rice plants are capable of inactivation of antioxidant enzymes of pathogens and that this ability favors disease resistance. C1 [Aver'yanov, A. A.; Zakharenkova, T. S.; Lapikova, V. P.; Pasechnik, T. D.] Russian Acad Agr Sci, Res Inst Phytopathol, Vyazemskii 143050, Moscow Region, Russia. [Gaivoronskaya, L. M.] Russian Peoples Friendship Univ, Fac Agr, Moscow 117198, Russia. [Baker, C. J.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Aver'yanov, AA (reprint author), Russian Acad Agr Sci, Res Inst Phytopathol, Vyazemskii 143050, Moscow Region, Russia. EM aaveryanov@post.ru FU Agricultural Research Service of the US Department of Agriculture [2682] FX The work was supported by the grant #2682 of the Agricultural Research Service of the US Department of Agriculture mediated by the International Science and Technology Center. NR 33 TC 1 Z9 1 U1 0 U2 17 PU MAIK NAUKA/INTERPERIODICA/SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 USA SN 1021-4437 EI 1608-3407 J9 RUSS J PLANT PHYSL+ JI Russ. J. Plant Physiol. PD MAR PY 2013 VL 60 IS 2 BP 270 EP 278 DI 10.1134/S1021443713020027 PG 9 WC Plant Sciences SC Plant Sciences GA 092BV UT WOS:000315094700013 ER PT J AU Liu, ZL Weber, SA Cotta, MA AF Liu, Z. Lewis Weber, Scott A. Cotta, Michael A. TI Isolation and Characterization of a beta-Glucosidase from a Clavispora Strain with Potential Applications in Bioethanol Production from Cellulosic Materials SO BIOENERGY RESEARCH LA English DT Article DE Cellobiose; Cellulosic ethanol; Simultaneous saccharification and fermentation; Yeast ID YEAST CANDIDA-WICKERHAMII; SACCHAROMYCES-CEREVISIAE; ETHANOL-PRODUCTION; SIMULTANEOUS SACCHARIFICATION; KINETIC CHARACTERIZATION; FUEL ETHANOL; PURIFICATION; FERMENTATION; CELLOBIOSE; GENE AB We previously reported on a new yeast strain of Clavispora sp. NRRL Y-50464 that is capable of utilizing cellobiose as sole source of carbon and energy by producing sufficient native beta-glucosidase enzyme activity without further enzyme supplementation for cellulosic ethanol production using simultaneous saccharification and fermentation. Eliminating the addition of external beta-glucosidase reduces the cost of cellulosic ethanol production. In this study, we present results on the isolation and identification of a beta-glucosidase protein from strain Y-50464. Using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and blast search of the NCBInr database (National Center for Biotechnology Information nonredundant), the protein from Y-50464 was identified as a beta-glucosidase (BGL1) with a molecular weight of 93.3 kDa. The BGL1 protein was purified through multiple chromatographic steps to a 26-fold purity (K (m) = 0.355 mM [pNPG]; K (i) = 15.2 mM [glucose]), which has a specific activity of 18.4 U/mg of protein with an optimal performance temperature at 45 A degrees C and pH of 6.0. This protein appears to be intracellular although other forms of the enzyme may exist. The fast growth rate of Y-50464 and its capability to produce sufficient beta-glucosidase activity for ethanol conversion from cellobiose provide a promising means for low-cost cellulosic ethanol production through a consolidated bioprocessing development. C1 [Liu, Z. Lewis; Weber, Scott A.; Cotta, Michael A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Liu, ZL (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM ZLewis.Liu@ars.usda.gov OI Cotta, Michael/0000-0003-4565-7754 NR 39 TC 4 Z9 4 U1 1 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 65 EP 74 DI 10.1007/s12155-012-9236-9 PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300007 ER PT J AU Pedersen, JF Sattler, SE Anderson, WF AF Pedersen, Jeffrey F. Sattler, Scott E. Anderson, William F. TI Evaluation of Public Sweet Sorghum A-Lines for Use in Hybrid Production SO BIOENERGY RESEARCH LA English DT Article DE Sweet sorghum; Hybrid; A-Line; Dwarf; Brix ID GENETIC-IMPROVEMENT; COMBINING ABILITY; PARENTAL LINES; REGISTRATION; QTL; TRAITS; SUGAR; STEM AB A fundamental need for commercialization of sweet sorghum [Sorghum bicolor (L.) Moench] as a bioenergy crop is an adequate seed supply, which will require development of hybrid varieties using dwarf seed-parent lines. A set of six public sweet sorghum A-lines (Dwarf Kansas Sourless, KS9, N36, N38, N39, and N4692) were crossed with a set of six public sweet sorghum cultivars (Brawley, Kansas Collier, Dale, Sugar Drip, Waconia, and Wray). Grain, fiber, and sugar yields were determined, and conversion formulas were applied to estimate ethanol yields. Hybrids were grown in fields at Ithaca, NE, USA, in 1983-1984 fertilized with 112 kg ha(-1) N. In terms of yield components and overall ethanol yields, one A-line, N38, was inferior. Average total ethanol yields from hybrids made on the other A-lines were not significantly different, suggesting that any of those five A-lines could be useful seed-parents. With the exception of grain yield, cultivars used as pollen parents were among the highest-performing entries for all traits. For all traits directly contributing to total ethanol yield (grain yield, juice yield, % soluble solids, sugar yield, fiber yield), hybrids were also among the highest-performing entries. Results of this study demonstrate that hybrid sweet sorghum with performance criteria equivalent to existing sweet sorghum cultivars can be produced on the sweet sorghum seed-parent lines A-Dwarf Kansas Sourless, A-KS9, A-N36, A-N39, and A-N4692. Identification of specific seed-parent x pollen parent lines with characteristics best suited for particular growing regions and end-user needs will be critical for commercial hybrid development. C1 [Pedersen, Jeffrey F.; Sattler, Scott E.] ARS, USDA, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Anderson, William F.] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Pedersen, Jeffrey F.; Sattler, Scott E.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RP Sattler, SE (reprint author), ARS, USDA, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. EM Scott.Sattler@ars.usda.gov NR 32 TC 5 Z9 6 U1 2 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 91 EP 102 DI 10.1007/s12155-012-9231-1 PG 12 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300010 ER PT J AU Novak, JM Cantrell, KB Watts, DW AF Novak, J. M. Cantrell, K. B. Watts, D. W. TI Compositional and Thermal Evaluation of Lignocellulosic and Poultry Litter Chars via High and Low Temperature Pyrolysis SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Energy content; GRACEnet; Nuclear magnetic resonance; Thermogravimetric analysis ID VICTORIAN BROWN-COAL; BED FAST PYROLYSIS; COMBUSTION PROPERTIES; BIOMASS COMPONENTS; RENEWABLE ENERGY; TORREFIED WOOD; WHEAT-STRAW; PART II; BIOCHAR; CHARCOAL AB Inorganic elements in biomass feedstocks can influence thermochemical reactions as well as the resultant char's elemental, compositional, and thermal characteristics. Chars were produced using slow pyrolysis under low (a parts per thousand currency sign400A degrees C) and high (a parts per thousand yen500A degrees C) temperature regimes from sugarcane bagasse, peanut hulls, pecan shell, pine chips, poultry litter, and switchgrass. The chars and raw feedstocks were characterized for their elemental, structural, and thermal properties to ascertain the implications of feedstock selection and pyrolysis temperatures on these properties. Char mass yields from the six feedstocks ranged between 28% and 78% by weight while carbon yields ranged between 44% and 89%. In both instances, lower yields were obtained with increasing pyrolysis temperature. Higher pyrolysis temperatures (a parts per thousand yen500A degrees C) resulted in more neutral to alkaline chars possessing greater ash contents and increased aromatic character with narrow O/C and H/C ratios. A significant exponential curve response (r (2) = 0.87, P < 0.001) was revealed between char mass yields vs. pyrolysis temperature. All raw feedstocks and chars contained mixed amounts of macro-, micro-, and trace element concentrations. The higher heating values (HHV) tended to increase with heightened pyrolysis temperature with some chars producing > 30 MJ kg(-1). The chars' HHV values inversely correlated to their total ash and Cl content. Lignocelluloses chars had better thermal characteristics and lower ash quality concerns implying suitable service in thermal energy production. In contrast, poultry litter char had greater ash contents, medium HHV values, and contained corrosive inorganic elements, which rendered it problematic as a feedstock for thermal energy generation. C1 [Novak, J. M.; Cantrell, K. B.; Watts, D. W.] ARS, USDA, Coastal Plains Res Lab, Florence, SC 29501 USA. RP Novak, JM (reprint author), ARS, USDA, Coastal Plains Res Lab, 2611 W Lucas St, Florence, SC 29501 USA. EM jeff.novak@ars.usda.gov FU US Department of Agriculture, Agriculture Research Service under the ARS-GRACEnet project FX This publication is based on work supported by the US Department of Agriculture, Agriculture Research Service, and under the ARS-GRACEnet project. The authors express gratitude to collaborators who manufactured the chars used in this study and to Barry Glaz, Jerry H. Martin II and Sheeneka Green in sample preparation and analyses. NR 64 TC 16 Z9 16 U1 4 U2 85 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 114 EP 130 DI 10.1007/s12155-012-9228-9 PG 17 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300012 ER PT J AU Lingle, SE Tew, TL Rukavina, H Boykin, DL AF Lingle, Sarah E. Tew, Thomas L. Rukavina, Hrvoje Boykin, Deborah L. TI Post-harvest Changes in Sweet Sorghum II: pH, Acidity, Protein, Starch, and Mannitol SO BIOENERGY RESEARCH LA English DT Article DE Ethanol; Sorghum; Storage; Harvest method; Fermentation feedstock ID SUGARCANE DETERIORATION; PROCESSING TECHNIQUES; ETHANOL-PRODUCTION; JUICE QUALITY; MATURITY; STORAGE; STALK AB This experiment was conducted to evaluate the effect of four harvesting methods on juice quality and storability in sweet sorghum. Three cultivars (Dale, Theis, and M81-E) were harvested at 90, 115, and 140 days after planting. Stalks were stripped of leaves and topped at the peduncle, then divided into four treatments (whole stalk, 20- or 40-cm billets, or chopped). The sorghum was stored outside at ambient temperature in a shade tent, and juice was extracted from samples removed at 0, 1, 2, and 4 days after harvest. Changes in juice Brix and sugars were reported in an earlier paper (Lingle, Tew, Rukavina, Boykin, Post-harvest changes in sweet sorghum I: Brix and sugars, BioEnergy Research 5:158-167, 2012). In this paper, we report changes in juice pH, titratable acidity (TA), and protein, starch, and mannitol concentrations. Juice pH dropped rapidly after harvest in chopped sorghum, but changed little during 4 days of storage in whole stalks or billets. Similarly, TA increased with storage time in chopped samples, but was unchanged in whole stalks and billets. Protein concentration was highly variable, and no pattern with treatment or storage time could be discerned. In whole stalks and billets, starch content slowly decreased during storage, while in chopped samples starch appeared to increase. This was most likely a result of an increase in dextran synthesized by microorganisms in those samples, which was also detected by the enzymatic starch assay. The concentration of mannitol increased with storage time in chopped samples, but not in whole stalks or billets. Within a harvest date, pH was highly correlated with total sugar, while TA and mannitol were highly negatively correlated with total sugar. The results confirm that whole stalks and billets were little changed over 4 days of storage, while chopped sorghum was badly deteriorated 1 day after harvest. Changes in pH, TA, or mannitol could be used to measure deterioration in sweet sorghum after harvest. C1 [Lingle, Sarah E.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Rukavina, Hrvoje] Western Illinois Univ, Macomb, IL 61455 USA. [Boykin, Deborah L.] ARS, USDA, Mid S Area, Stoneville, MS 38776 USA. RP Lingle, SE (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sarah.lingle@ars.usda.gov NR 30 TC 3 Z9 3 U1 0 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 178 EP 187 DI 10.1007/s12155-012-9248-5 PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300017 ER PT J AU Headlee, WL Zalesny, RS Donner, DM Hall, RB AF Headlee, William L. Zalesny, Ronald S., Jr. Donner, Deahn M. Hall, Richard B. TI Using a Process-Based Model (3-PG) to Predict and Map Hybrid Poplar Biomass Productivity in Minnesota and Wisconsin, USA SO BIOENERGY RESEARCH LA English DT Article DE 3-PG; Geographic information system; Populus; Site quality; Yield ID SHORT-ROTATION COPPICE; LIGHT-USE EFFICIENCY; FOREST GROWTH-MODEL; POPULUS-DELTOIDES; CO2 ENRICHMENT; WOODY BIOMASS; UNITED-STATES; ENERGY CROPS; CLONES; PHOTOSYNTHESIS AB Hybrid poplars have demonstrated high biomass productivity in the North Central USA as short rotation woody crops (SRWCs). However, our ability to quantitatively predict productivity for sites that are not currently in SRWCs is limited. As a result, stakeholders are also limited in their ability to evaluate different areas within the region as potential supply sheds for wood-based bioenergy facilities. A reliable method for predicting productivity across the region is needed; preferably, such a method will also lend itself to generating yield maps that stakeholders can use to inform their decision making. In this study, the Physiological Processes Predicting Growth model was (1) assigned parameters for hybrid poplars using species-specific physiological data and allometric relationships from previously-published studies, (2) calibrated for the North Central region using previously-published biomass data from eight plantations along with site-specific climate and soils data, (3) validated against previously published biomass data from four other plantations using linear regression of actual versus predicted total aboveground dry biomass (R (2) = 0.89, RMSE = 8.1 Mg ha(-1), mean bias = 5.3 Mg ha(-1)), (4) evaluated for sensitivity of the model to manipulation of the parameter for age at full canopy cover (fullCanAge) and the fertility rating growth modifier, and (5) combined with soil and climate data layers to produce a map of predicted biomass productivity for the states of Minnesota and Wisconsin. Mean annual biomass productivity (total aboveground dry biomass divided by age) ranged from 4.4 to 13.0 Mg ha(-1) year(-1) across the states, with the highest productivity mainly concentrated in the area stretching from south-central Minnesota across southern Wisconsin. C1 [Headlee, William L.; Hall, Richard B.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Zalesny, Ronald S., Jr.; Donner, Deahn M.] US Forest Serv, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI 54501 USA. RP Headlee, WL (reprint author), Iowa State Univ, Dept Nat Resource Ecol & Management, 339 Sci 2, Ames, IA 50011 USA. EM wheadlee@iastate.edu FU US Forest Service; Institute for Applied Ecosystem Studies (IAES); Wisconsin FOCUS ON ENERGY Program FX This work was jointly funded by the US Forest Service, Institute for Applied Ecosystem Studies (IAES) and the Wisconsin FOCUS ON ENERGY Program. The authors would like to thank the Commonwealth Scientific and Industrial Research Organization (CSIRO) for providing the 3-PG software used in the study. We are also grateful to Sue Lietz and Tina Baumann of the IAES for substantial contributions with data management and GIS mapping procedures; Dan Langseth and Mike Young of Verso Paper for their expert knowledge on hybrid poplar commercial production and management; and David Coyle of the University of Georgia, Philip Dixon of Iowa State University, and Steven Jungst of Iowa State University, for providing expert review of earlier drafts of this manuscript. NR 57 TC 10 Z9 10 U1 0 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 196 EP 210 DI 10.1007/s12155-012-9251-x PG 15 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300019 ER PT J AU Yelle, DJ Kaparaju, P Hunt, CG Hirth, K Kim, H Ralph, J Felby, C AF Yelle, Daniel J. Kaparaju, Prasad Hunt, Christopher G. Hirth, Kolby Kim, Hoon Ralph, John Felby, Claus TI Two-Dimensional NMR Evidence for Cleavage of Lignin and Xylan Substituents in Wheat Straw Through Hydrothermal Pretreatment and Enzymatic Hydrolysis SO BIOENERGY RESEARCH LA English DT Article DE Wheat straw; Hydrothermal; Lignin; Polysaccharides; O-acetyls; beta-aryl ethers; Uronic acids; Cinnamates ID SOLUTION-STATE NMR; FERULATE CROSS-LINKS; PLANT-CELL WALLS; WOOD; GRASSES; IDENTIFICATION; SPECTROSCOPY; CELLULOSE; FIBER; MODEL AB Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy of plant cell walls is a powerful tool for characterizing changes in cell wall chemistry during the hydrothermal pretreatment process of wheat straw for second-generation bioethanol production. One-bond C-13-H-1 NMR correlation spectroscopy, via an heteronuclear single quantum coherence experiment, revealed substantial lignin beta-aryl ether cleavage, deacetylation via cleavage of the natural acetates at the 2-O- and 3-O-positions of xylan, and uronic acid depletion via cleavage of the (1 -> aEuro parts per thousand 2)-linked 4-O-methyl-alpha-d-glucuronic acid of xylan. In the polysaccharide anomeric region, decreases in the minor beta-d-mannopyranosyl, and alpha-l-arabinofuranosyl units were observed in the NMR spectra from hydrothermally pretreated wheat straw. The aromatic region indicated only minor changes to the aromatic structures during the process (e.g., further deacylation revealed by the depletion in ferulate and p-coumarate structures). Supplementary chemical analyses showed that the hydrothermal pretreatment increased the cellulose and lignin concentration with partial removal of extractives and hemicelluloses. The subsequent enzymatic hydrolysis incurred further deacetylation of the xylan, leaving approximately 10 % of acetate intact based on the weight of original wheat straw. C1 [Yelle, Daniel J.; Hunt, Christopher G.; Hirth, Kolby] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Kaparaju, Prasad; Felby, Claus] Univ Copenhagen, Fac Life Sci, Frederiksberg, Denmark. [Kim, Hoon; Ralph, John] Univ Wisconsin, Dept Biochem, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53705 USA. [Kim, Hoon; Ralph, John] Univ Wisconsin, Wisconsin Bioenergy Initiat, Madison, WI USA. [Kaparaju, Prasad] Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland. RP Yelle, DJ (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM dyelle@fs.fed.us OI Felby, Claus/0000-0002-6537-0155 FU Danish National Advanced Technology Foundation [18708]; DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494] FX The Danish National Advanced Technology Foundation is greatly acknowledged for funding the project "Development of 2nd generation bioethanol process and technology" Project No. 18708. We also gratefully acknowledge the ARS Dairy Forage Research Center, Madison, Wisconsin for use of their NMR spectrometer in the early stages of this research. JR and HK were funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). NR 70 TC 29 Z9 29 U1 6 U2 128 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 211 EP 221 DI 10.1007/s12155-012-9247-6 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300020 ER PT J AU Kiniry, JR Anderson, LC Johnson, MVV Behrman, KD Brakie, M Burner, D Cordsiemon, RL Fay, PA Fritschi, FB Houx, JH Hawkes, C Juenger, T Kaiser, J Keitt, TH Lloyd-Reilley, J Maher, S Raper, R Scott, A Shadow, A West, C Wu, Y Zibilske, L AF Kiniry, J. R. Anderson, L. C. Johnson, M. -V. V. Behrman, K. D. Brakie, M. Burner, D. Cordsiemon, R. L. Fay, P. A. Fritschi, F. B. Houx, J. H., III Hawkes, C. Juenger, T. Kaiser, J. Keitt, T. H. Lloyd-Reilley, J. Maher, S. Raper, R. Scott, A. Shadow, A. West, C. Wu, Y. Zibilske, L. TI Perennial Biomass Grasses and the Mason-Dixon Line: Comparative Productivity across Latitudes in the Southern Great Plains SO BIOENERGY RESEARCH LA English DT Article DE Biofuel grasses; Switchgrass; Miscanthus; Simulation modeling ID SWITCHGRASS PANICUM-VIRGATUM; USE-EFFICIENCY; WATER-USE; POPULATIONS; PLANT; ADAPTATION; MAIZE; MODEL; MISCANTHUS; FEEDSTOCK AB Understanding latitudinal adaptation of switchgrass (Panicum virgatum L.) and Miscanthus (Miscanthus x giganteus J. M. Greef & Deuter ex Hodk. & Renvoize) to the southern Great Plains is key to maximizing productivity by matching each grass variety to its optimal production environment. The objectives of this study were: (1) to quantify latitudinal variation in production of representative upland switchgrass ecotypes (Blackwell, Cave-in-Rock, and Shawnee), lowland switchgrass ecotypes (Alamo, Kanlow), and Miscanthus in the southern half of the US Great Plains and (2) to investigate the environmental factors affecting yield variation. Leaf area and yield were measured on plots at 10 locations in Missouri, Arkansas, Oklahoma, and Texas. More cold winter days led to decreased subsequent Alamo switchgrass yields and increased subsequent upland switchgrass yields. More hot-growing season days led to decreased Kanlow and Miscanthus yields. Increased drought intensity also contributed to decreased Miscanthus yields. Alamo switchgrass had the greatest radiation use efficiency (RUE) with a mean of 4.3 g per megajoule intercepted PAR and water use efficiency (WUE) with a mean of 4.5 mg of dry weight per gram of water transpired. The representative RUE values for other varieties ranged from 67 to 80 % of Alamo's RUE value and 67 to 87 % of Alamo's WUE. These results will provide valuable inputs to process-based models to realistically simulate these important perennial grasses in this region and to assess the environmental impacts of production on water use and nutrient demands. In addition, it will also be useful for landowners and companies choosing the most productive perennial grasses for biofuel production. C1 [Kiniry, J. R.; Behrman, K. D.; Fay, P. A.] ARS, USDA, Temple, TX USA. [Anderson, L. C.; Behrman, K. D.; Hawkes, C.; Juenger, T.; Keitt, T. H.] Univ Texas Austin, Austin, TX 78712 USA. [Johnson, M. -V. V.] USDA, NRCS, Temple, TX USA. [Brakie, M.; Shadow, A.] USDA, NRCS E Texas Plant Mat Ctr, Nacogdoches, TX USA. [Burner, D.] ARS, USDA, Houoma, LA USA. [Burner, D.; Raper, R.] ARS, USDA, Booneville, AR USA. [Raper, R.; Wu, Y.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Cordsiemon, R. L.; Kaiser, J.] USDA, NRCS Elsberry Plant Mat Ctr, Elsberry, MO USA. [Fritschi, F. B.; Houx, J. H., III] Univ Missouri, Columbia, MO USA. [Lloyd-Reilley, J.; Maher, S.] USDA, NRCS Kika de la Garza Plant Mat Ctr, Kingsville, TX USA. [Scott, A.] Rio Farms Inc, Monte Alto, TX USA. [West, C.] Texas Tech Univ, Lubbock, TX 79409 USA. [West, C.] Univ Arkansas, Fayetteville, AR 72701 USA. [Zibilske, L.] ARS, USDA, Weslaco, TX USA. RP Kiniry, JR (reprint author), ARS, USDA, Temple, TX USA. EM Jim.Kiniry@ARS.USDA.GOV OI Fay, Philip/0000-0002-8291-6316 NR 37 TC 15 Z9 16 U1 2 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 276 EP 291 DI 10.1007/s12155-012-9254-7 PG 16 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300026 ER PT J AU Sattler, SE Palmer, NA Saballos, A Greene, AM Xin, ZG Sarath, G Vermerris, W Pedersen, JF AF Sattler, Scott E. Palmer, Nathan A. Saballos, Ana Greene, Ann M. Xin, Zhanguo Sarath, Gautam Vermerris, Wilfred Pedersen, Jeffrey F. TI Identification and Characterization of Four Missense Mutations in Brown midrib12 (Bmr12), the Caffeic acid O-Methyltranferase (COMT) of Sorghum (vol 5, pg 855, 2012) SO BIOENERGY RESEARCH LA English DT Correction C1 [Sattler, Scott E.; Palmer, Nathan A.; Sarath, Gautam; Pedersen, Jeffrey F.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Sattler, Scott E.; Palmer, Nathan A.; Sarath, Gautam; Pedersen, Jeffrey F.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Saballos, Ana; Greene, Ann M.; Vermerris, Wilfred] Univ Florida, Dept Agron, Gainesville, FL 32610 USA. [Saballos, Ana; Greene, Ann M.; Vermerris, Wilfred] Univ Florida, Genet Inst, Gainesville, FL 32610 USA. [Xin, Zhanguo] USDA ARS, Plant Stress & Germplasm Dev Unit, Lubbock, TX 79415 USA. RP Sattler, SE (reprint author), Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. EM Scott.Sattler@ars.usda.gov NR 1 TC 0 Z9 0 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2013 VL 6 IS 1 BP 402 EP 403 DI 10.1007/s12155-012-9239-6 PG 2 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 084EA UT WOS:000314518300037 ER PT J AU Renye, JA Somkuti, GA AF Renye, John A., Jr. Somkuti, George A. TI BlpC-regulated bacteriocin production in Streptococcus thermophilus SO BIOTECHNOLOGY LETTERS LA English DT Article DE Bacteriocin; Quorum sensing; Streptococcus thermophilus; Thermophilin 110 ID COMPARATIVE GENOMICS; SEQUENCE; PLASMID; GENE AB Streptococcus thermophilus B59671 produces a bacteriocin with anti-pediococcal activity, but genes required for its production are not characterized. Genome sequencing of S. thermophilus has identified a genetic locus encoding a quorum sensing (QS) system that regulates production of class II bacteriocins. However, in strains possessing this gene cluster, production of bacteriocin like peptides (Blp) was only observed when excess pheromone was provided. PCR analysis revealed this strain possessed blpC, which encodes the 30-mer QS pheromone. To investigate if BlpC regulates bacteriocin production in S. thermophilus B59671, an integrative vector was used to replace blpC with a gene encoding for kanamycin resistance and the resulting mutant did not inhibit the growth of Pediococcus acidilactici. Constitutive expression of blpC from a shuttle vector restored the bacteriocin production, confirming the blp gene cluster is essential for bacteriocin activity in S. thermophilus B59671. C1 [Renye, John A., Jr.; Somkuti, George A.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Renye, JA (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM john.renye@ars.usda.gov NR 24 TC 4 Z9 4 U1 0 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD MAR PY 2013 VL 35 IS 3 BP 407 EP 412 DI 10.1007/s10529-012-1095-0 PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 087UG UT WOS:000314788400015 PM 23183916 ER PT J AU Cerda, A Brazier, R Nearing, M de Vente, J AF Cerda, Artemi Brazier, Richard Nearing, Mark de Vente, Joris TI Scales and erosion Preface SO CATENA LA English DT Editorial Material ID SEDIMENT DELIVERY PROBLEM; SOIL-EROSION; TRANSPORT; YIELD; WATER; MODELS; BASIN; PREDICTION; CATCHMENTS; ISSUES C1 [Cerda, Artemi] Univ Valencia, Soil Eros & Degradat Res Grp SEDER, Dept Geog, Valencia 46010, Spain. [Brazier, Richard] Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4RJ, Devon, England. [Nearing, Mark] USDA ARS, SW Watershed Res Ctr, Tucson, AZ USA. [de Vente, Joris] CSIC, EEZA, Desertificat & Geoecol Dept, La Canada De San Urbano 04120, Almeria, Spain. RP Cerda, A (reprint author), Univ Valencia, Soil Eros & Degradat Res Grp SEDER, Dept Geog, Blasco Ibanez 28, Valencia 46010, Spain. EM artemio.cerda@uv.es RI de Vente, Joris/C-1304-2012; Cerda, Artemi/I-4670-2013; Brazier, Richard/N-1806-2013 OI de Vente, Joris/0000-0001-7428-0621; Cerda, Artemi/0000-0001-5326-4489; NR 39 TC 29 Z9 29 U1 1 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 J9 CATENA JI Catena PD MAR PY 2013 VL 102 SI SI BP 1 EP 2 DI 10.1016/j.catena.2011.09.006 PG 2 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA 083QJ UT WOS:000314480000001 ER PT J AU Beuzelin, JM Wilson, LT Showler, AT Meszaros, A Wilson, BE Way, MO Reagan, TE AF Beuzelin, J. M. Wilson, L. T. Showler, A. T. Meszaros, A. Wilson, B. E. Way, M. O. Reagan, T. E. TI Oviposition and larval development of a stem borer, Eoreuma loftini, on rice and non-crop grass hosts SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE oviposition preference; larval developmental performance; free amino acids; Lepidoptera; Crambidae; Oryza sativa; Poaceae ID SACCHARINA WALKER LEPIDOPTERA; CHILO-PARTELLUS; SPODOPTERA-EXIGUA; SUGARCANE BORER; PLANT NITROGEN; CRAMBIDAE; PREFERENCE; PYRALIDAE; RESISTANCE; COTTON AB A greenhouse study compared oviposition preference and larval development duration of a stem borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), on rice, Oryza sativa L. cv Cocodrie (Poaceae), and four primary non-crop hosts of Texas Gulf Coast rice agroecosystems. Rice and two perennials, johnsongrass, Sorghum halepense (L.) Pers., and vaseygrass, Paspalum urvillei Steud. (both Poaceae), were assessed at three phenological stages. Two spring annuals, brome, Bromus spec., and ryegrass, Lolium spec. (both Poaceae), were assessed at two phenological stages. Phenological stages represented the diversity of plant development stages E.loftini may encounter. Plant fresh biomass, dry biomass, and sum of tiller heights were used as measures of plant availability. Accounting for plant availability, rice was preferred over non-crop hosts, and intermediate and older plants were preferred over young plants. Johnsongrass and vaseygrass were 3260% as preferred as rice when considering the most preferred phenological stages of each host. Brome and ryegrass received few or no eggs, respectively. Eoreuma loftini larval development (in degree days above developmental threshold temperatures) was fastest on rice and slowest on johnsongrass and vaseygrass. Development duration was only retarded by plant stage on young rice plants. Foliar and stem free amino acid concentrations were determined to help provide insights on the mechanisms of E.loftini oviposition preference and developmental performance. C1 [Beuzelin, J. M.; Meszaros, A.; Wilson, B. E.; Reagan, T. E.] Louisiana State Univ, Dept Entomol, Ctr Agr, Baton Rouge, LA 70803 USA. [Wilson, L. T.; Way, M. O.] Texas A&M Univ, Texas A&M AgriLife Res & Extens Ctr, Beaumont, TX 77713 USA. [Showler, A. T.] USDA ARS, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Beuzelin, JM (reprint author), Dean Lee Res Stn, 8105 Tom Bowman Dr, Alexandria, LA 71302 USA. EM jbeuzelin@agcenter.lsu.edu RI Wilson, Lloyd/E-9971-2011 FU USDA CSREES Crops-At-Risk IPM program [2008-51100-04415]; USDA NIFA AFRI Sustainable Bioenergy program [2011-67009-30132] FX This work was supported by USDA CSREES Crops-At-Risk IPM program grant 2008-51100-04415 and USDA NIFA AFRI Sustainable Bioenergy program grant 2011-67009-30132. We thank David Blouin, Mike Stout, Rita Riggio, Bill White, Lee Tarpley, Ronnie Porter, Veronica Abrigo, Jaime Cavazos, Becky Pearson, Sebe Brown, Jannie Castillo, and Jiale Lv for their technical assistance. We thank David Blouin, Mike Stout, and Eric Webster for participating in the review of the manuscript. This study is approved for publication by the Director of the Louisiana Agricultural Experiment Station as manuscript number 2012-263-7423. NR 41 TC 7 Z9 7 U1 2 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAR PY 2013 VL 146 IS 3 BP 332 EP 346 DI 10.1111/eea.12031 PG 15 WC Entomology SC Entomology GA 086DJ UT WOS:000314663200003 ER PT J AU Buckman, KA Campbell, JF AF Buckman, Karrie A. Campbell, James F. TI How varying pest and trap densities affect Tribolium castaneum capture in pheromone traps SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE red flour beetle; monitoring; modeling; stored products; pest management; Coleoptera; Tenebrionidae; aggregation pheromone ID PLODIA-INTERPUNCTELLA LEPIDOPTERA; STORED-GRAIN INSECTS; SEX-PHEROMONE; BAITED TRAPS; FLOUR MILL; TENEBRIONIDAE; COLEOPTERA; MOTH; POPULATIONS; ATTRACTION AB The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is an important insect pest in food processing facilities. Pheromone trapping is frequently used to monitor red flour beetle populations in structures; however, the optimal trap density and the relationship between trap captures and beetle density is not known. Two experiments were performed concurrently in environmentally controlled 30-m2 walk-in chambers to determine the relationship between aggregation pheromone trap captures of red flour beetles and beetle and trap number. In one experiment, beetle density was kept constant at 200 individuals per chamber while trap number was varied from 1 to 8, and in the other experiment trap number remained constant at one per chamber while beetle density varied from 20 to 800 individuals. Results indicated that approximately one out of 23 red flour beetles were captured in a trap. Number of beetles captured in traps increased significantly as beetle density increased; however, the proportion of beetles captured remained consistent across beetle densities with a mean of 4.7 +/- 0.6% of individuals captured. Trap captures varied significantly with trap placement within experimental chambers, indicating that subtle differences in the trapping environment can influence trap captures. Data suggested that trap densities of 0.070.10m2 (23 traps per chamber) would maximize trap capture, whereas a trap density of 0.13m2 (four traps per chamber) would maximize the predictive ability of a trapping equation estimating beetle density from trap captures. Results provide information needed to more thoroughly explore how environmental factors might influence red flour beetle trap capture in the absence of changes in beetle density. Further understanding of these relationships will allow for more accurate assessments of absolute beetle density from pheromone trap capture data. C1 [Buckman, Karrie A.; Campbell, James F.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. RP Campbell, JF (reprint author), ARS, Ctr Grain & Anim Hlth Res, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov NR 28 TC 3 Z9 3 U1 3 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAR PY 2013 VL 146 IS 3 BP 404 EP 412 DI 10.1111/eea.12039 PG 9 WC Entomology SC Entomology GA 086DJ UT WOS:000314663200010 ER PT J AU Yauk, CL Bishop, J Dearfield, KL Douglas, GR Hales, BF Luijten, M O'Brien, JM Robaire, B Sram, R van Benthem, J Wade, MG White, PA Marchetti, F AF Yauk, Carole L. Bishop, Jack Dearfield, Kerry L. Douglas, George R. Hales, Barbara F. Luijten, Mirjam O'Brien, Jason M. Robaire, Bernard Sram, Radim van Benthem, Jan Wade, Mike G. White, Paul A. Marchetti, Francesco TI The development of adverse outcome pathways for mutagenic effects for the organization for economic co-operation and development SO ENVIRONMENTAL AND MOLECULAR MUTAGENESIS LA English DT Editorial Material DE germ cells; mutagenesis; genotoxicity testing ID GERM-CELL MUTAGENS; CHALLENGES; GUIDELINES; REACH C1 [Yauk, Carole L.; Douglas, George R.; O'Brien, Jason M.; Wade, Mike G.; White, Paul A.; Marchetti, Francesco] Hlth Canada, Environm Hlth Sci & Res Bur, Ottawa, ON K1A 0L2, Canada. [Bishop, Jack] NIEHS, Natl Toxicol Program, Res Triangle Pk, NC 27709 USA. [Dearfield, Kerry L.] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Hales, Barbara F.; Robaire, Bernard] McGill Univ, Dept Obstet & Gynecol, Dept Pharmacol & Therapeut, Montreal, PQ H3A 2T5, Canada. [Luijten, Mirjam; van Benthem, Jan] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands. [Sram, Radim] Acad Sci Czech Republic, Inst Expt Med, Prague, Czech Republic. RP Yauk, CL (reprint author), Hlth Canada, Environm Hlth Sci & Res Bur, Ottawa, ON K1A 0L2, Canada. EM carole.yauk@hc-sc.gc.ca RI Sram, Radim/H-2455-2014; OI Sram, Radim/0000-0003-4256-3816; Marchetti, Francesco/0000-0002-9435-4867; Wade, Michael/0000-0002-7331-3839; Yauk, Carole/0000-0003-4919-876X; white, paul/0000-0001-5853-4759 NR 14 TC 4 Z9 4 U1 0 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-6692 J9 ENVIRON MOL MUTAGEN JI Environ. Mol. Mutagen. PD MAR PY 2013 VL 54 IS 2 BP 79 EP 81 DI 10.1002/em.21757 PG 3 WC Environmental Sciences; Genetics & Heredity; Toxicology SC Environmental Sciences & Ecology; Genetics & Heredity; Toxicology GA 085EB UT WOS:000314595400001 PM 23355186 ER PT J AU Vencl, FV Srygley, RB AF Vencl, Fredric V. Srygley, Robert B. TI Enemy targeting, trade-offs, and the evolutionary assembly of a tortoise beetle defense arsenal SO EVOLUTIONARY ECOLOGY LA English DT Article DE Acromis sparsa; Community assembly; Modes of selection; Escalation; Shield; Gregariousness; Guild; Maternal care; Multiple species interactions; Trade-offs ID SHIELD DEFENSE; PLANT DEFENSE; HOST-PLANT; PREDATORS; BEHAVIOR; INSECTS; ECOLOGY; ANTS; GREGARIOUSNESS; HETEROPTERA AB In response to intense enemy selection, immature folivorous insects have evolved elaborate, multi-trait defense arsenals. How enemies foster trait diversification and arsenal assembly depends on which selective mode they impose: whether different enemies select for the same defense or exert conflicting selection on a prey species. Theory has long supposed that the selective advantage of a defense depends on its efficacy against a broad spectrum of enemies, which implies that predator selection is more diffuse than pairwise. Here, we use the multi-trait defense arsenal of the tortoise beetle, Acromis sparsa, which consists of shields, gregariousness and maternal guarding to test whether: (1) diverse enemies have selected for narrowly targeted defenses in the Acromis lineage; (2) newer traits out-performed older ones or vice versa, and; (3) if selection by different enemies results in positive (escalation) trends in defense effectiveness. Because their defenses could be modified or ablated, individuals were rendered differentially protected, and their survival was quantified in a long-term field study. Exclusion experiments evaluated defense efficacy against particular enemy guilds. Logit regression revealed that: (1)no single trait increased survival against the entire enemy suite; (2)trait efficacy was strongly correlated with a particular enemy, consistent with narrow targeting; (3)traits lacked strong cross-resistance among enemies; (4)traits performed synergistically, consistent with the idea of escalation, and; (5)traits interacted negatively to decrease survival, indicative of performance trade-offs. From collation of the phylogenetic histories of arsenal and enemy community assembly we hypothesize that older traits performed better against older enemies and that patterns of both trait and enemy accumulation are consistent with defense escalation. Trade-offs and lack of cross-resistance among defenses imply that enemy selection has been conflicting at the guild level and that negative functional interactions among defenses have fostered the evolution of a defense arsenal of increasing complexity. C1 [Vencl, Fredric V.] SUNY Stony Brook, Stony Brook, NY 11794 USA. [Vencl, Fredric V.; Srygley, Robert B.] Smithsonian Trop Res Inst, Balboa, Ancon, Panama. [Srygley, Robert B.] ARS, USDA, No Plains Agr Res Lab, Sidney, MT 59270 USA. RP Vencl, FV (reprint author), SUNY Stony Brook, Stony Brook, NY 11794 USA. EM fvencl@life.bio.sunysb.edu NR 59 TC 7 Z9 7 U1 0 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0269-7653 J9 EVOL ECOL JI Evol. Ecol. PD MAR PY 2013 VL 27 IS 2 BP 237 EP 252 DI 10.1007/s10682-012-9603-1 PG 16 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 084FZ UT WOS:000314524900001 ER PT J AU Regazzoni, L Del Vecchio, L Altomare, A Yeum, KJ Cusi, D Locatelli, F Carini, M Aldini, G AF Regazzoni, L. Del Vecchio, L. Altomare, A. Yeum, K. -J. Cusi, D. Locatelli, F. Carini, M. Aldini, G. TI Human serum albumin cysteinylation is increased in end stage renal disease patients and reduced by hemodialysis: mass spectrometry studies SO FREE RADICAL RESEARCH LA English DT Article DE end stage renal diseases; albumin; Cys34; cysteinylation; mass spectrometry; hemodialysis; oxidative damage ID OXIDATIVE STRESS; PLASMA-ALBUMIN; REDOX STATE; POSSIBLE BIOMARKER; REACTIVE ALDEHYDE; DIABETES-MELLITUS; CYSTEINE; ACID; ATHEROSCLEROSIS; SULFIREDOXIN AB The aim of the present work was to monitor the covalent modifications of human serum albumin (HSA) in end stage renal diseases (ESRD) non-diabetic patients, before and after hemodialysis (HD), by direct infusion electrospray mass spectrometry (ESI-MS). Human serum samples were collected from healthy subjects (n = 10, 20-60 yr) and age-matched ESRD patients (n = 8) before and after HD, purified by affinity chromatography and analyzed by a triple-quadrupole mass spectrometer. The deconvoluted spectra from healthy subjects were all characterized by three peaks attributed to non-glycated mercaptoalbumin (HSA-SH) and to the corresponding adducts with cysteine (HSA-Cys) and glucose (HSA-Glc); relative contents: mercaptoalbumin in both glycated and non-glycated form, HSA-SHt (74 + 6%), HSA-Cys (26 +/- 5%) and HSA-Glc (24 +/- 3%). HSA isolated from ESRD patients before HD was characterized by a significant reduction of HSA-SHt (42 +/- 7%), and by a concomitant increase of the HSA-Cys adduct (58 +/- 7%). Hemodialysis significantly reduced the cysteinylated form (37 +/- 7%) and restored HSA-SHt (63 + 8%) in all the ESRD patients. The mechanism of thiol oxidation and cysteinylation was then studied by mass spectrometry, using LQQCPF as a model peptide and H2O2 as an oxidizing agent. C1 [Regazzoni, L.; Altomare, A.; Carini, M.; Aldini, G.] Univ Milan, Dept Pharmaceut Sci, I-20133 Milan, Italy. [Del Vecchio, L.; Locatelli, F.] Osped Alessandro Manzoni, Dept Nephrol Dialysis & Renal Transplant, Lecce, Italy. [Yeum, K. -J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Cusi, D.] Univ Milan, Fdn Filarete, Dept Hlth Sci Genom & Bioinformat Platform, I-20133 Milan, Italy. RP Aldini, G (reprint author), Univ Milan, Dept Pharmaceut Sci, Via L Mangiagalli 25, I-20133 Milan, Italy. EM giancarlo.aldini@unimi.it RI Regazzoni, Luca/D-4212-2014; CARINI, MARINA/D-3084-2015 OI Regazzoni, Luca/0000-0001-7199-7141; aldini, giancarlo/0000-0002-2355-6744; CARINI, MARINA/0000-0003-3407-5425 FU Regione Lombardia (Technological and Scientific Cooperation Agreement) [SAL-60, 16749]; BioGreen 21 Program [20070301034009]; Rural Development Administration, Korea; US Department of Agriculture [58-1950-7-707] FX This work was supported by Regione Lombardia (Technological and Scientific Cooperation Agreement, ID SAL-60, Ref n. 16749).; KJ Yeum has been supported in part by the BioGreen 21 Program (Code #20070301034009), Rural Development Administration, Korea and US Department of Agriculture, under Agreement 58-1950-7-707. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 52 TC 12 Z9 12 U1 0 U2 20 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 1071-5762 J9 FREE RADICAL RES JI Free Radic. Res. PD MAR PY 2013 VL 47 IS 3 BP 172 EP 180 DI 10.3109/10715762.2012.756139 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 085XV UT WOS:000314648500005 PM 23215783 ER PT J AU Landis, AMG Haag, WR Stoeckel, JA AF Landis, Andrew M. Gascho Haag, Wendell R. Stoeckel, James A. TI High suspended solids as a factor in reproductive failure of a freshwater mussel SO FRESHWATER SCIENCE LA English DT Article DE Ligumia subrostrata; eutrophication; total suspended solids; fertilization; reproduction; freshwater mussel; Unionidae ID DREISSENA-POLYMORPHA; FILTRATION-RATES; ZEBRA MUSSEL; UNIONIDAE; BIVALVIA; SEDIMENT; NITROGEN; DYNAMICS; AMMONIA; RIVER AB Elevated suspended solids are a widespread stressor of aquatic ecosystems, but their effects on growth and reproduction in freshwater mussels are largely unknown. We fertilized experimental ponds to create a gradient in total suspended solids (TSS) and examined the effects of TSS on growth, nutritional status, reproduction, and clearance rate in Ligumia subrostrata. The number of females that became gravid declined sharply with increasing TSS, and no gravid females were found in the highest TSS treatments. The proportion of gravid females was not related to the TSS organic:inorganic ratio. Fertilization was an all-or-nothing phenomenon. In all females that did become gravid, 98 to 99% of eggs were fertilized regardless of TSS, and total fecundity was unrelated to TSS. Clearance rates declined sharply as TSS increased but showed a threshold relationship in which clearance was uniformly low at TSS > similar to 8 mg/L. Reproductive failure probably was not caused by poor body condition or nutritional status because growth (length and mass) and energetic status (measured as caloric density) were not related to TSS. We propose 2 mechanisms that implicate interference of TSS with fertilization as the cause of reproductive failure. Reduced clearance rate could decrease the chance of females encountering suspended sperm during filter feeding, or an increase in pseudofeces production could bind sperm in mucus and lead to its egestion before fertilization. Interruption of fertilization coincident with high TSS is a potential mechanism to explain the lack of mussel recruitment in many locations. Monitoring and reduction of TSS, especially during the spawning season, may help create conditions necessary for maintenance and recovery of mussel populations. More research is needed to explore the generality of this pattern across a broad range of mussel species including those adapted to lotic environments or that use different brooding strategies. C1 [Landis, Andrew M. Gascho; Stoeckel, James A.] Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA. [Haag, Wendell R.] ARS, USDA, Ctr Bottomland Hardwood Res, Forest Hydrol Lab, Oxford, MS 38655 USA. RP Landis, AMG (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, 203 Swingle Hall, Auburn, AL 36849 USA. EM andrewmgl@gmail.com; whaag@fs.fed.us; jimstoeckel@auburn.edu NR 57 TC 7 Z9 7 U1 4 U2 74 PU SOC FRESWATER SCIENCE PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2013 VL 32 IS 1 BP 70 EP 81 DI 10.1899/12-093.1 PG 12 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 086FZ UT WOS:000314670800006 ER PT J AU Isaak, DJ Rieman, BE AF Isaak, Daniel J. Rieman, Bruce E. TI Stream isotherm shifts from climate change and implications for distributions of ectothermic organisms SO GLOBAL CHANGE BIOLOGY LA English DT Review DE climate change; climate velocity; distribution shift; fish; global warming; isotherm; monitoring; range contraction; stream temperature ID FRESH-WATER BIODIVERSITY; TROUT SALVELINUS-FONTINALIS; POTENTIAL HABITAT LOSS; NONNATIVE BROOK TROUT; BROWN TROUT; SPECIES DISTRIBUTIONS; AIR-TEMPERATURE; CUTTHROAT TROUT; BULL TROUT; THERMAL HABITAT AB Stream ecosystems are especially vulnerable to climate warming because most aquatic organisms are ectothermic and live in dendritic networks that are easily fragmented. Many bioclimatic models predict significant range contractions in stream biotas, but subsequent biological assessments have rarely been done to determine the accuracy of these predictions. Assessments are difficult because model predictions are either untestable or so imprecise that definitive answers may not be obtained within timespans relevant for effective conservation. Here, we develop the equations for calculating isotherm shift rates (ISRs) in streams that can be used to represent historic or future warming scenarios and be calibrated to individual streams using local measurements of stream temperature and slope. A set of reference equations and formulas are provided for application to most streams. Example calculations for streams with lapse rates of 0.8 similar to degrees C/100 similar to m and long-term warming rates of 0.10.2 similar to degrees C similar to decade-1 indicate that isotherms shift upstream at 0.131.3 similar to km similar to decade-1 in steep streams (210% slope) and 1.325 similar to km similar to decade-1 in flat streams (0.11% slope). Used more generally with global scenarios, the equations predict isotherms shifted 1.543 similar to km in many streams during the 20th Century as air temperatures increased by 0.6 similar to degrees C and would shift another 5143 similar to km in the first half of the 21st Century if midrange projections of a 2 similar to degrees C air temperature increase occur. Variability analysis suggests that short-term variation associated with interannual stream temperature changes will mask long-term isotherm shifts for several decades in most locations, so extended biological monitoring efforts are required to document anticipated distribution shifts. Resampling of historical sites could yield estimates of biological responses in the short term and should be prioritized to validate bioclimatic models and develop a better understanding about the effects of temperature increases on stream biotas. C1 [Isaak, Daniel J.] US Forest Serv, Rocky Mt Res Stn, Boise Aquat Sci Lab, Boise, ID USA. [Rieman, Bruce E.] US Forest Serv, Rocky Mt Res Stn, Seeley Lake, MT USA. RP Isaak, DJ (reprint author), US Forest Serv, Rocky Mt Res Stn, Boise Aquat Sci Lab, 322 E Front St,Suite 401, Boise, ID USA. EM disaak@fs.fed.us FU US Forest Service, Rocky Mountain Research Station; Washington Office of Research and Development FX This research was supported by the US Forest Service, Rocky Mountain Research Station, and the Washington Office of Research and Development. An earlier draft of this manuscript was improved by comments from Mike Young, Seth Wenger, Frank Rahel, Dan Gibson-Reinemer, and two anonymous reviewers. Thanks go to Dona Horan for her preparation of Fig. 4 and Dan Evans for his high school trigonometry class. NR 110 TC 36 Z9 36 U1 2 U2 95 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2013 VL 19 IS 3 BP 742 EP 751 DI 10.1111/gcb.12073 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 080CV UT WOS:000314219700007 PM 23504832 ER PT J AU Moraes, MG Chatterton, NJ Harrison, PA Filgueiras, TS Figueiredo-Ribeiro, RCL AF Moraes, M. G. Chatterton, N. J. Harrison, P. A. Filgueiras, T. S. Figueiredo-Ribeiro, R. C. L. TI Diversity of non-structural carbohydrates in grasses (Poaceae) from Brazil SO GRASS AND FORAGE SCIENCE LA English DT Article DE Cerrado; non-structural carbohydrates; starch; tropical grasses ID TALL FESCUE CULTIVARS; PERENNIAL RYEGRASS; COOL TEMPERATURES; METABOLISM; FRUCTAN; STARCH; PLANTS; EXCHANGE; SUCROSE; STRESS AB Non-structural carbohydrates (NSC) are the primary energy available for growth and dry-mass production in forage grasses. They are also associated with tolerance to environmental stresses, including drought. Soluble carbohydrates, especially fructans of temperate forage grasses, have been extensively studied; however, little is known about NSC of tropical grasses. These plants are abundant in the Cerrado, a savanna-like vegetation with a definite seasonality in rainfall distribution. Such an environment presents an ideal condition for the occurrence of fructans. However, the present analysis of carbohydrates in aerial parts of twenty-four tropical grass species, mainly from the Panicoideae subfamily in the Cerrado, shows a distinctively different NSC profile when compared with temperate grasses. Free glucose and starch are common NSC found in most plant organs. However, free fructose and sucrose-based oligosaccharides were only found in very low amounts. Sucrose was detected in higher amounts only in stems. A linear series of malto-oligosaccharides was found in leaf blades, sheaths and stems of most of the Cerrado grasses, except for those from the Andropogoneae tribe. These results indicate a diversity of regulatory pathways of NSC metabolism in tropical forage grasses from the Cerrado. C1 [Moraes, M. G.] Univ Fed Goias, Inst Ciencias Biol, BR-74001970 Goiania, Go, Brazil. [Chatterton, N. J.; Harrison, P. A.] ARS, Forage & Range Res Lab, USDA, Logan, UT USA. [Filgueiras, T. S.] 3 Herbarium Maria Eneyda P Kauffman Fidalgo Inst, Sao Paulo, Brazil. [Figueiredo-Ribeiro, R. C. L.] Inst Bot, Nucleo Fisiol & Bioquim Plantas 4, Sao Paulo, Brazil. RP Moraes, MG (reprint author), Univ Fed Goias, Inst Ciencias Biol, Cx P 131, BR-74001970 Goiania, Go, Brazil. EM moemy@icb.ufg.br RI Moraes, Moemy/G-1215-2010 OI Moraes, Moemy/0000-0002-2217-1199 FU FAPESP [Proc. 00/06422-4, 2005/04139-7]; CAPES FX This work is part of the Ph.D. thesis of the first author and was supported by FAPESP (Proc. 00/06422-4 and 2005/04139-7). M. G. Moraes thanks CAPES for the Ph.D. fellowship. R. C. L. Figueiredo-Ribeiro and T. S. Filgueiras are researchers associated to CNPq. We also thank Dr M. A. M. Carvalho (Institute of Botany, Sao Paulo) for the English revision and many helpful suggestions regarding this manuscript. NR 41 TC 2 Z9 2 U1 3 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0142-5242 J9 GRASS FORAGE SCI JI Grass Forage Sci. PD MAR PY 2013 VL 68 IS 1 BP 165 EP 177 DI 10.1111/j.1365-2494.2012.00883.x PG 13 WC Agronomy SC Agriculture GA 083PH UT WOS:000314476800014 ER PT J AU Michaud, GA Monger, HC Anderson, DL AF Michaud, G. A. Monger, H. C. Anderson, D. L. TI Geomorphic-vegetation relationships using a geopedological classification system, northern Chihuahuan Desert, USA SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Arid-semiarid ecosystems; Desert ecology; Desert geomorphology; Desert soils; Desert vegetation; Gypsum soils; Jornada Experimental Range; White Sands ID SOUTHERN NEW-MEXICO; SEMIDESERT GRASSLAND RANGE; SOIL PROPERTIES; SEMIARID LANDSCAPES; PLANT-COMMUNITIES; OVERLAND-FLOW; ROOT SYSTEMS; SCALE; DYNAMICS; ARIZONA AB Drylands typically have distinctive vegetative patterns that reflect the underlying physical landscape. We use a geopedological classification to organize the landscape into five categories from broad scale to fine scale: (1) physiographic divisions which describe regional topography, such as mountains and basin floors; (2) regolith type which identifies residuum versus transported sediments; (3) parent materials which describe mineralogy; (4) landforms which provide meso-scale descriptions of topography; and (5) soil texture, a fine-scale variable important for infiltration, erodibility, and available water holding capacity. The study was conducted in a 1753 km(2) area of the White Sands Missile Range and easternmost Jornada Experimental Range and revealed the following. (1) At the broadest scale, mesquite is dominant on the basin floor when the parent material is siliceous sand, but (2) biological soil crust becomes dominant in the basin floor when the parent material is gypseous. (3) Creosotebush is dominant on rocky soils of the piedmont slopes regardless of parent material, but (4) grasslands become dominant on the bajadas upslope in the semiarid zone and into the semiarid mountain uplands regardless of bedrock type. This method provides a way of supplementing ecologic-edaphic studies and provides a framework within which mechanisms can be explored. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Michaud, G. A.] USDA Nat Resource Conservat Serv, Las Cruces, NM 88011 USA. [Monger, H. C.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Monger, HC (reprint author), New Mexico State Univ, Las Cruces, NM 88003 USA. EM cmonger@nmsu.edu FU Jornada Basin LTER program [DEB-94111971]; New Mexico State University Agricultural Experiment Station; U.S. Army Environmental Center FX This research was supported in part by an appointment to the Student Environmental Management Participation Program at the U.S. Army Environmental Center administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Army Environmental Center. Additional support was provided by the Jornada Basin LTER program (DEB-94111971) and the New Mexico State University Agricultural Experiment Station. Comments by April Ulery, Jeff Herrick, Brandon Bestelmeyer, David Rachal, and Gretchen Michaud are greatly appreciated. NR 100 TC 4 Z9 4 U1 5 U2 71 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 J9 J ARID ENVIRON JI J. Arid. Environ. PD MAR PY 2013 VL 90 BP 45 EP 54 DI 10.1016/j.jaridenv.2012.10.001 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 083SN UT WOS:000314485600007 ER PT J AU Ferrareze, JP Fugate, KK Bolton, MD Deckard, EL Campbell, LG Finger, FL AF Ferrareze, Jocleita Peruzzo Fugate, Karen Klotz Bolton, Melvin D. Deckard, Edward L. Campbell, Larry G. Finger, Fernando L. TI Jasmonic acid does not increase oxidative defense mechanisms or common defense-related enzymes in postharvest sugarbeet roots SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Beta vulgaris; Disease resistance; Induced resistance; Jasmonate; Reactive oxygen species; Storage duration ID METHYL JASMONATE; DISEASE RESISTANCE; SALICYLIC-ACID; ANTIOXIDANT ACTIVITY; BIOCHEMICAL-CHANGES; CHILLING INJURY; PROTEIN GENES; PEACH FRUIT; STORAGE; EXPRESSION AB Jasmonic acid (JA) treatment significantly reduces rot due to several sugarbeet (Beta vulgaris L.) storage pathogens. However, the mechanisms by which JA protects postharvest sugarbeet roots from disease are unknown. In other plant species and organs, alterations in antioxidant defense mechanisms and elevations in common pathogenesis-related defense enzymes have been implicated in jasmonate-induced disease resistance. To investigate whether these mechanisms are involved in JA-induced disease resistance in stored sugarbeet roots, the activities of several reactive oxygen species (ROS)-scavenging and pathogenesis-related defense enzymes and the total concentration of antioxidant compounds were determined in harvested sugarbeet roots in the 60 d following treatment with JA. ROS-scavenging and pathogenesis-related defense enzymes and the concentration of antioxidant compounds were largely unaffected by JA as JA-treated roots exhibited small declines in superoxide dismutase (SOD) and chitinase activities, and were generally unaltered in ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), beta-1,3-glucanase (beta-Gluc), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) activities or antioxidant compounds concentration. The lack of increase in enzyme activities or metabolites related to defense against oxidative stress or pathogens suggests that JA-induced disease resistance in postharvest sugarbeet roots does not arise from a direct increase in any of the ROS-scavenging and defense-related enzymes examined, or the concentration of total antioxidant compounds. However, ROS-scavenging enzymes and pathogenesis-related defense enzymes were affected by storage duration with POD, SOD, beta-Gluc, chitinase, and PPO activities elevated and APX and CAT activities reduced in roots stored for 10 d or more. Storage-related changes in activities of ROS-scavenging enzymes and defense-related enzymes provide further evidence that these enzymes are uninvolved in sugarbeet root disease resistance during storage since many of these enzymes increased in activity after prolonged storage when disease resistance generally declines. Published by Elsevier B.V. C1 [Ferrareze, Jocleita Peruzzo] Univ Fed Vicosa, Dept Biol Vegetal, BR-36571000 Vicosa, MG, Brazil. [Fugate, Karen Klotz; Bolton, Melvin D.; Campbell, Larry G.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Deckard, Edward L.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Finger, Fernando L.] Univ Fed Vicosa, Dept Fitotecnia, BR-36571000 Vicosa, MG, Brazil. RP Fugate, KK (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM karen.fugate@ars.usda.gov OI Fugate, Karen/0000-0001-9543-6832 FU CNPq (Brazil); Beet Sugar Development Foundation FX The authors thank John Eide for technical assistance, CNPq (Brazil) for J.P.F.'s scholarship, and the Beet Sugar Development Foundation for partial financial support of this research. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 43 TC 7 Z9 7 U1 1 U2 67 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2013 VL 77 BP 11 EP 18 DI 10.1016/j.postharvbio.2012.11.003 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA 086FV UT WOS:000314670400003 ER PT J AU Imahori, Y Yamamoto, K Tanaka, H Bai, JH AF Imahori, Yoshihiro Yamamoto, Kohei Tanaka, Hiroshi Bai, Jinhe TI Residual effects of low oxygen storage of mature green fruit on ripening processes and ester biosynthesis during ripening in bananas SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Banana; Low-oxygen atmosphere; Alcohol acetyltransferase; Alcohol dehydrogenase; Ester; Volatile ID ETHANOL VAPOR; SHELF-LIFE; AROMA COMPOUNDS; QUALITY; ATMOSPHERES; ETHYLENE; ACETALDEHYDE; VOLATILES; RESPONSES; NITROGEN AB Mature green banana (Musa sapientum L cv. Cavendish) fruit were stored in 0.5%, 2%, or 21% O-2 for 7 days at 20 degrees C before ripening was initiated by ethylene. Residual effects of low O-2 storage in mature green fruit on ripening and ester biosynthesis in fruit were investigated during ripening for up to 6 d at 20 degrees C. Concentrations of ethanol in mature green fruit did not change during storage in both 21% and 2% O-2 atmospheres, but increased in fruit stored in 0.5% O-2. The activities of alcohol dehydrogenase (ADH) in 2% and 21%O-2 atmospheres remained very low throughout the storage period, but significantly increased with 0.5% O-2. After transferring fruit to regular air and trigging ripening with ethylene, yellowing of peel, fruit softening and hydrolysis of starch in fruit stored in low O-2 atmospheres were slower than in the control. Fruit stored in low O-2 also showed a delayed onset of the climacteric peak. The activities of ADH were lower in the low O-2 stored fruit than in the control fruit. Productions of ethyl acetate, isoamyl acetate, and isobutyl acetate were remarkably suppressed by low O-2 storage. Alcohol acetyltransferase activity increased gradually with storage time in all treatments, being significantly lower in fruit with low O-2 pretreatments. The results indicate that low O-2 plus room temperature storage can extend storage life of bananas with the sacrifice of a low production of ester volatiles. (C) 2012 Elsevier B.V. All rights reserved. C1 [Imahori, Yoshihiro; Yamamoto, Kohei; Tanaka, Hiroshi] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Lab Postharvest Physiol, Naka Ku, Sakai, Osaka 5998531, Japan. [Bai, Jinhe] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. RP Imahori, Y (reprint author), Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Lab Postharvest Physiol, Naka Ku, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan. EM imahori@plant.osakafu-u.ac.jp FU Japanese Society for the Promotion Science [20580037] FX This work was supported in part by a Grant-in-Aid for Scientific Research (No. 20580037) from Japanese Society for the Promotion Science. NR 37 TC 7 Z9 10 U1 2 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2013 VL 77 BP 19 EP 27 DI 10.1016/j.postharvbio.2012.11.004 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA 086FV UT WOS:000314670400004 ER PT J AU Erasmus, A Lennox, CL Smilanick, JL Lesar, K Fourie, PH AF Erasmus, Arno Lennox, Cheryl L. Smilanick, Joseph L. Lesar, Keith Fourie, Paul H. TI Imazalil residue loading and green mould control on citrus fruit as affected by formulation, solution pH and exposure time in aqueous dip treatments SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Imazalil sulphate; Fungicide resistance; Penicillium digitatum; Citrus ID 50 DEGREES-C; PENICILLIUM-DIGITATUM; FUNGICIDE RESISTANCE; INHIBIT GERMINATION; DECAY CONTROL; RIND WOUNDS; LEMONS; PYRIMETHANIL; CALIFORNIA; VARIANCE AB Green mould, caused by Penicillium digitatum, is responsible for major postharvest fruit losses on the South African fresh citrus export market. Some of these losses as well as fungicide resistance development can be attributed to sub-optimal imazalil (IMZ) residue loading on citrus fruit (<2 mu g g(-1)), which is commonly the case in South African packhouses. This will result in loss of control and sporulation inhibition on decayed fruit. IMZ formulation [IMZ sulphate and emulsifiable concentrate (EC)], solution pH (IMZ sulphate at 500 mu g mL(-1) buffered with NaHCO3 or NaOH to pH Land 8) and exposure time (15-540s) were investigated in order to improve IMZ residue loading and the green mould control on Clementine mandarin, 'Eureka' lemon, and navel and Valencia orange fruit. Exposure time had no significant effect on residue loading in the unbuffered IMZ sulphate solution (pH 3). No differences were observed between the pH buffers used, but residue loading improved with increase in pH. The maximum residue limit (MRL) of 5.0 mu g g(-1) was exceeded following dip treatment in the IMZ EC (after 75 s exposure time), and IMZ sulphate at pH 8 using NaHCO3 (77 s) or NaOH (89 s) as buffer. The MRL was exceeded after 161 s in IMZ sulphate solutions buffered at pH 6 with either NaHCO3 or NaOH. An IMZ residue-loading curve was prepared from which residue levels can be predicted for the control of IMZ-sensitive and IMZ-resistant isolates of P. digitatum. From this model the benchmark residue level for 95% control of an IMZ-sensitive isolate and of an IMZ-resistant isolate were predicted to be 0.81 and 2.64 mu g g(-1), respectively. Residue loading can be improved by adjusting the pH level of an IMZ sulphate solution to 6 or by using the IMZ EC formulation, but exposure time should be restricted to 45 s so as not to exceed the MRL. Conversely, sufficient exposure time of approximate to 90 s in an unbuffered IMZ sulphate solution (pH 3) will result to improved green mould control, but with residue loading below 2 mu g g(-1). The resistant isolate could not be controlled adequately with residue levels below the MRL, therewith indicating the practical relevance of IMZ resistance. (C) 2012 Elsevier B.V. All rights reserved. C1 [Erasmus, Arno; Lesar, Keith; Fourie, Paul H.] Citrus Res Int, Nelspruit, South Africa. [Erasmus, Arno; Lennox, Cheryl L.; Fourie, Paul H.] Univ Stellenbosch, Dept Plant Pathol, ZA-7600 Stellenbosch, South Africa. [Smilanick, Joseph L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. RP Erasmus, A (reprint author), Citrus Res Int, 2 Baker St, Nelspruit, South Africa. EM arno@cri.co.za FU Citrus Research International, Citrus Academy, Postharvest Innovation Fund; THRIP FX The authors thank Citrus Research International, Citrus Academy, Postharvest Innovation Fund and THRIP for financial support; personnel at the Department of Plant Pathology, University Stellenbosch for assistance; Hearshaw and Kinnes Analytical Laboratory (Pty) Ltd. for residue analyses; Marde Booyse (Genetic and physiological biometrician, Biometry unit, ARC-Infruitec, Stellenbosch) for statistical analysis, ICA International Chemicals (Pty) Ltd. for chemicals and Hennie Jordaan from Imagichem for giving more insight into the chemistry of imazalil. NR 31 TC 7 Z9 8 U1 0 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2013 VL 77 BP 43 EP 49 DI 10.1016/j.postharvbio.2012.11.001 PG 7 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA 086FV UT WOS:000314670400007 ER PT J AU Ramsay, TG Stoll, MJ Conde-Aguilera, JA Caperna, TJ AF Ramsay, T. G. Stoll, M. J. Conde-Aguilera, J. A. Caperna, T. J. TI Peripheral tumor necrosis factor alpha regulation of adipose tissue metabolism and adipokine gene expression in neonatal pigs SO VETERINARY RESEARCH COMMUNICATIONS LA English DT Article DE Neonate; Adipokine; Pig; Tumor necrosis factor; Interleukin 6 ID LIPOPROTEIN-LIPASE ACTIVITY; INFLAMMATION-RELATED ADIPOKINES; MIGRATION INHIBITORY FACTOR; TNF-ALPHA; ADIPOCYTES; ADIPONECTIN; SECRETION; GLUCOSE; SWINE; GLUCOCORTICOIDS AB The neonatal pig is susceptible to stress and infection, conditions which favor tumor necrosis factor alpha (TNF alpha) secretion. This study examined whether TNF alpha can alter metabolic activity and cytokine gene expression within neonatal pig adipose tissue. Cell cultures were prepared from neonatal subcutaneous adipose tissue using standard procedures. Cultures (5 experiments) were incubated with medium containing C-14-glucose for 4 h to measure glucose conversion to lipid in the presence of combinations of TNF alpha (10 ng), insulin (10 nM) and an anti-pig TNF alpha antibody (5 mu g). Basal lipogenesis was not affected by TNF alpha treatment (P > 0.05). However, insulin stimulated lipogenesis was reduced by TNF alpha (P < 0.02). For gene expression studies, cultures were incubated with 0, 2.5, 5.0 or 10 ng TNF alpha for 2, 4 or 24 h (n = 4 experiments). Interleukin 6 and TNF alpha gene expression were acutely (2-4 h) stimulated by exogenous TNF alpha treatment (P < 0.05), as analyzed by real-time PCR. Adiponectin mRNA abundance was reduced (P < 0.001) while monocyte chemotactic gene expression was increased by TNF alpha treatment at all time points (P < 0.001). Chronic treatment (24 h) was required to increase monocyte multiplication inhibitory factor or suppress lipoprotein lipase gene expression (P < 0.02). These data suggest conditions which increase serum TNF alpha, like sepsis, could suppress lipid accumulation within adipose tissue at a time of critical need in the neonate and induce a variety of adipose derived cytokines which may function to alter adipose physiology. C1 [Ramsay, T. G.; Stoll, M. J.; Caperna, T. J.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA,BARC E, Beltsville, MD 20705 USA. [Conde-Aguilera, J. A.] CSIC, Estn Expt Zaidin, Dept Physiol & Biochem Anim Nutr, Inst Anim Nutr, Granada, Spain. RP Ramsay, TG (reprint author), ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA,BARC E, Bldg 200,Rm 207, Beltsville, MD 20705 USA. EM timothy.ramsay@ars.usda.gov NR 36 TC 1 Z9 2 U1 0 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-7380 J9 VET RES COMMUN JI Vet. Res. Commun. PD MAR PY 2013 VL 37 IS 1 BP 1 EP 10 DI 10.1007/s11259-012-9540-z PG 10 WC Veterinary Sciences SC Veterinary Sciences GA 086XK UT WOS:000314722700001 PM 23090779 ER PT J AU Gao, ZQ Cao, XM Gao, W AF Gao, Zhiqiang Cao, Xiaoming Gao, Wei TI The spatio-temporal responses of the carbon cycle to climate and land use/land cover changes between 1981-2000 in China SO FRONTIERS OF EARTH SCIENCE LA English DT Article DE carbon cycle; climate changes; LULC; remote sensing; Earth system modeling ID NET PRIMARY PRODUCTION; TERRESTRIAL ECOSYSTEMS; PRIMARY PRODUCTIVITY; ATMOSPHERIC CO2; INTERANNUAL VARIATIONS; DYNAMIC-RESPONSES; UNITED-STATES; BALANCE; STORAGE; PATTERNS AB This paper represents the first national effort of its kind to systematically investigate the impact of changes in climate and land use and land cover (LULC) on the carbon cycle with high-resolution dynamic LULC data at the decadal scale (1990s and 2000s). Based on simulations using well calibrated and validated Carbon Exchanges in the Vegetation-Soil-Atmosphere (CEVSA) model, temporal and spatial variations in carbon storage and fluxes in China may be generated empower us to relate these variations to climate variability and LULC with respect to net primary productivity (NPP), heterotrophic respiration (HR), net ecosystem productivity (NEP), storage and soil carbon (SOC), and vegetation carbon (VEGC) individually or collectively. Overall, the increases in NPP were greater than HR in most cases due to the effect of global warming with more precipitation in China from 1981 to 2000. With this trend, the NEP remained positive during that period, resulting in a net increase of total amount of carbon being stored by about 0.296 PgC within a 20-year time frame. Because the climate effect was much greater than that of changes of LULC, the total carbon storage in China actually increased by about 0.17 PgC within the 20-year time period. Such findings will contribute to the generation of carbon emissions control policies under global climate change impacts. C1 [Gao, Zhiqiang] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [Cao, Xiaoming] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Gao, Zhiqiang; Gao, Wei] Colorado State Univ, Nat Resource Ecol Lab, USDA UV B Monitoring & Res Program, Ft Collins, CO 80521 USA. RP Cao, XM (reprint author), Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. EM caoxm@lreis.ac.cn RI Gao, Wei/O-1208-2013; Gao, Wei/C-1430-2016 FU National Natural Science Foundation of China [41171334, 41071278]; USDA NIFA Project [2010-34263-21075] FX This work was supported by the National Natural Science Foundation of China (Grant Nos. 41171334 and 41071278) and USDA NIFA Project (No. 2010-34263-21075). The authors acknowledge all the financial support gratefully. NR 48 TC 2 Z9 5 U1 2 U2 103 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2095-0195 J9 FRONT EARTH SCI-PRC JI Front. Earth Sci. PD MAR PY 2013 VL 7 IS 1 BP 92 EP 102 DI 10.1007/s11707-012-0335-x PG 11 WC Geosciences, Multidisciplinary SC Geology GA 080YD UT WOS:000314279400009 ER PT J AU Johnston, CA Moreno, JP El-Mubasher, A Gallagher, M Tyler, C Woehle, D AF Johnston, Craig A. Moreno, Jennette P. El-Mubasher, Abeer Gallagher, Martina Tyler, Chermaine Woehle, Deborah TI Impact of a School-Based Pediatric Obesity Prevention Program Facilitated by Health Professionals SO JOURNAL OF SCHOOL HEALTH LA English DT Article DE pediatric obesity; intervention; prevention; school-based ID LATINO PRESCHOOL-CHILDREN; BODY-MASS INDEX; PHYSICAL-ACTIVITY; CHILDHOOD OBESITY; ACADEMIC-ACHIEVEMENT; COORDINATED APPROACH; ADOLESCENT GIRLS; US CHILDREN; OVERWEIGHT; INTERVENTIONS AB BACKGROUND This study evaluated a school-based obesity intervention for elementary school children (N?=?835) where health professionals assisted teachers with the integration of healthy messages into the school curriculum. METHODS Schools were randomized into a professional-facilitated intervention (PFI; N?=?4) or a self-help (SH; N?=?3) condition. Changes in weight-based outcomes were assessed in students enrolled in the second grade from all 7 schools (overall: N?=?835 students; PFI: N?=?509 students, SH: N?=?326 students). Students were between ages 7 and 9 and from diverse ethnic backgrounds (Asian?=?25.3%, Black?=?23.3%, Hispanic?=?23.1%, White?=?28.3%). The sample included 321 overweight/obese (BMI?=?85th percentile), 477 normal-weight (BMI?=?5th percentile and <85th percentile), and 37 underweight (BMI?17,500 J/g) even though the properties of carbonized pellets, such as bulk density, unit density and inorganic ash, had poor quality compared with untreated pellets. Carbonized bamboo pellets are the proposed new biomass solid fuel and have the potential to be developed as commercial pellets. (C) 2012 Published by Elsevier Ltd. C1 [Liu, Zhijia; Jiang, Zehui; Fei, Benhua; Yu, Yan; Liu, Xing'e] Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. [Liu, Zhijia; Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. RP Jiang, ZH (reprint author), Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. EM feibenhua@icbr.ac.cn FU 'Basic Scientific Research Funds of International Centre for Bamboo and Rattan' [1632012002]; 'Development and demonstration of bamboo/wood composite LVL and wallboard' [[200814]] FX This research was financially supported by the 'Basic Scientific Research Funds of International Centre for Bamboo and Rattan' (Grant No. 1632012002) and 'Development and demonstration of bamboo/wood composite LVL and wallboard' (Grant No. [200814]). NR 32 TC 14 Z9 14 U1 2 U2 43 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD MAR PY 2013 VL 51 BP 1 EP 6 DI 10.1016/j.renene.2012.07.034 PG 6 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA 062LT UT WOS:000312922000001 ER PT J AU Jeon, S Lim, J Inglett, GE Lee, S AF Jeon, Soyoung Lim, Jongbin Inglett, George E. Lee, Suyong TI Effect of enzymatic treatments on the rheological and oil-resisting properties of wheat flour-based frying batters SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Frying; Enzyme; Oil uptake; Rheology ID FRIED CHICKEN NUGGETS; MICROBIAL TRANSGLUTAMINASE; RICE FLOUR; CARROT SLICES; QUALITY; TEXTURE; BREAD; SOY; FUNCTIONALITY; ABSORPTION AB A new enzymatic approach was introduced to reduce the oil uptake of batter-coated fried foods. Crosslinking (transglutaminase) and cell wall-degrading (viscozyme) enzymes were incorporated into the formulation of wheat flour-based frying batters, and their rheological/oil-resisting effects were evaluated. The transglutaminase treatment increased the pasting viscosities of wheat flour whereas viscozyme distinctly reduced these properties. The batters treated with transglutaminase exhibited the highest steady-shear viscosity, followed by the control and viscozyme-treated samples. Dynamic viscoelastic results showed that transglutaminase improved the elastic properties of frying batters, while their viscous characteristics became more dominant when viscozyme was used. These rheological features were favorably correlated with the adhesive properties of batters. During frying, the moisture loss of frying batters was retarded by transglutaminase. The incorporation of transglutaminase reduced the oil uptake of frying batters by 19.6%, thus contributing to the enhancement of the oil-barrier property. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Jeon, Soyoung; Lim, Jongbin; Lee, Suyong] Sejong Univ, Dept Food Sci & Technol, Seoul 143747, South Korea. [Jeon, Soyoung; Lim, Jongbin; Lee, Suyong] Sejong Univ, Carbohydrate Bioprod Res Ctr, Seoul 143747, South Korea. [Inglett, George E.] ARS, Cereal Prod & Food Sci Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Lee, S (reprint author), Sejong Univ, Dept Food Sci & Technol, 98 Gunja Dong, Seoul 143747, South Korea. EM suyonglee@sejong.ac.kr FU National Research Foundation of Korea (NRF); Korea government (MEST) [2010-0008483] FX This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0008483). NR 32 TC 2 Z9 2 U1 5 U2 56 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2013 VL 115 IS 2 BP 215 EP 219 DI 10.1016/j.jfoodeng.2012.10.015 PG 5 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 065GB UT WOS:000313135400009 ER PT J AU Leiva-Valenzuela, GA Lu, RF Aguilera, JM AF Leiva-Valenzuela, Gabriel A. Lu, Renfu Miguel Aguilera, Jose TI Prediction of firmness and soluble solids content of blueberries using hyperspectral reflectance imaging SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Hyperspectral reflectance imaging; Image processing; Quality sorting; Blueberry; Firmness; Soluble solids content ID APPLE FRUIT; NONDESTRUCTIVE MEASUREMENT; NIR SPECTROSCOPY; QUALITY; SEPARATION; SELECTION; MODULUS AB Currently, blueberries are inspected and sorted by color, size and/or firmness (or softness) in packing houses, using different inspection techniques like machine vision and mechanical vibration or impact. A new inspection technique is needed for effectively assessing both external features and internal quality attributes of individual blueberries. This paper reports on the use of hyperspectral imaging technique for predicting the firmness and soluble solids content (SSC) of blueberries. A pushbroom hyperspectral imaging system was used to acquire hyperspectral reflectance images from 302 blueberries in two fruit orientations (i.e., stem and calyx ends) for the spectral region of 500-1000 nm. Mean spectra were extracted from the regions of interest for the hyperspectral images of each blueberry. Prediction models were developed based on partial least squares method using cross validation and were externally tested with 25% of the samples. Better firmness predictions (R = 0.87) were obtained, compared to SSC predictions (R = 0.79). Fruit orientation had no or insignificant effect on the firmness and SSC predictions. Further analysis showed that blueberries could be sorted into two classes of firmness. This research has demonstrated the feasibility of implementing hyperspectral imaging technique for sorting blueberries for firmness and possibly SSC to enhance the product quality and marketability. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Leiva-Valenzuela, Gabriel A.; Miguel Aguilera, Jose] Pontificia Univ Catolica Chile, Dept Chem & Bioproc Engn, Santiago, Chile. [Leiva-Valenzuela, Gabriel A.; Lu, Renfu] Michigan State Univ, USDA, ARS, E Lansing, MI 48824 USA. RP Leiva-Valenzuela, GA (reprint author), Pontificia Univ Catolica Chile, Dept Chem & Bioproc Engn, Ave Vicuna Mackenna 4860, Santiago, Chile. EM gmleiva@uc.cl RI Leiva-Valenzuela, Gabriel/F-8970-2013 OI Leiva-Valenzuela, Gabriel/0000-0002-2490-865X FU National Commission for Science and Technology (CONICYT) of Chile FX Mr. Gabriel A. Leiva-Valenzuela thanks The National Commission for Science and Technology (CONICYT) of Chile for providing a fellowship, which allowed him to carry out the research in the USDA/ARS postharvest engineering laboratory at Michigan State University in USA. The authors also acknowledge helpful discussions with Dr. Fernando Mendoza, Research Agricultural Engineer with USDA/ARS at East Lansing, Michigan, and valuable suggestions in performing the experiment from Dr. Haiyan Cen, and statistical analysis from Mr. Irwin Donis-Gonzalez and Mr. Ahmed Rady, graduate students in Biosystems Engineering at Michigan State University. NR 30 TC 49 Z9 51 U1 9 U2 115 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2013 VL 115 IS 1 BP 91 EP 98 DI 10.1016/j.jfoodeng.2012.10.001 PG 8 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 057QB UT WOS:000312577000011 ER PT J AU Joly, R Forcella, F Peterson, D Eklund, J AF Joly, Rozenn Forcella, Frank Peterson, Dean Eklund, James TI Planting depth for oilseed calendula SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Calendic acid; Emergence; Hydrothermal time; Seeding depth; Seed oil ID SEED-GERMINATION; OFFICINALIS; EMERGENCE; TEMPERATURE; CROP; OIL AB Calendula (Calendula officinalis L) is a popular ornamental and medicinal plant, but it also is a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Current agronomic information on calendula is not easily available, is limited in geographic scope, or pertains primarily to ornamental or medicinal varieties. Consequently, our objective was to investigate seedling establishment of oilseed calendula in response to planting depth and soil microclimate in field soils over two years in central Minnesota, USA. 'Carola' was used in all experiments; it is one of the few commercial oilseed varieties available. More seedlings emerged from planting depths of 1 and 2 cm than from 4 or 6 cm. Regardless of planting depth, time after planting to 50% emergence was less variable when estimated by hydrothermal time (HTT, 89 degrees C d, CV = 14) than calendar days (7 d, CV = 39). HIT was calculated best with a base temperature of 5.5 degrees C and a base water potential of -2900 kPa (-2.9 MPa). Thus, growers must plant calendula at 1-2 cm, but soil at this depth can dry rapidly, which slows accumulation of HTT and delays emergence of calendula seedlings. Published by Elsevier B.V. C1 [Forcella, Frank; Peterson, Dean; Eklund, James] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Joly, Rozenn] Ecole Super Agr, F-49007 Angers 01, France. RP Forcella, F (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. EM frank.forcella@ars.usda.gov NR 22 TC 6 Z9 7 U1 3 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2013 VL 42 BP 133 EP 136 DI 10.1016/j.indcrop.2012.05.016 PG 4 WC Agricultural Engineering; Agronomy SC Agriculture GA 015ZT UT WOS:000309487700019 ER PT J AU Howe, GT Yu, JB Knaus, B Cronn, R Kolpak, S Dolan, P Lorenz, WW Dean, JFD AF Howe, Glenn T. Yu, Jianbin Knaus, Brian Cronn, Richard Kolpak, Scott Dolan, Peter Lorenz, W. Walter Dean, Jeffrey F. D. TI A SNP resource for Douglas-fir: de novo transcriptome assembly and SNP detection and validation SO BMC GENOMICS LA English DT Article ID GENOME-WIDE ASSOCIATION; PINUS-TAEDA L.; PSEUDOTSUGA-MENZIESII; COMPLEX TRAITS; DNA-SEQUENCES; WHITE SPRUCE; SEED ORCHARD; SELECTION; GENERATION; DATABASE AB Background: Douglas-fir (Pseudotsuga menziesii), one of the most economically and ecologically important tree species in the world, also has one of the largest tree breeding programs. Although the coastal and interior varieties of Douglas-fir (vars. menziesii and glauca) are native to North America, the coastal variety is also widely planted for timber production in Europe, New Zealand, Australia, and Chile. Our main goal was to develop a SNP resource large enough to facilitate genomic selection in Douglas-fir breeding programs. To accomplish this, we developed a 454-based reference transcriptome for coastal Douglas-fir, annotated and evaluated the quality of the reference, identified putative SNPs, and then validated a sample of those SNPs using the Illumina Infinium genotyping platform. Results: We assembled a reference transcriptome consisting of 25,002 isogroups (unique gene models) and 102,623 singletons from 2.76 million 454 and Sanger cDNA sequences from coastal Douglas-fir. We identified 278,979 unique SNPs by mapping the 454 and Sanger sequences to the reference, and by mapping four datasets of Illumina cDNA sequences from multiple seed sources, genotypes, and tissues. The Illumina datasets represented coastal Douglas-fir (64.00 and 13.41 million reads), interior Douglas-fir (80.45 million reads), and a Yakima population similar to interior Douglas-fir (8.99 million reads). We assayed 8067 SNPs on 260 trees using an Illumina Infinium SNP genotyping array. Of these SNPs, 5847 (72.5%) were called successfully and were polymorphic. Conclusions: Based on our validation efficiency, our SNP database may contain as many as similar to 200,000 true SNPs, and as many as similar to 69,000 SNPs that could be genotyped at similar to 20,000 gene loci using an Infinium II array-more SNPs than are needed to use genomic selection in tree breeding programs. Ultimately, these genomic resources will enhance Douglas-fir breeding and allow us to better understand landscape-scale patterns of genetic variation and potential responses to climate change. C1 [Howe, Glenn T.; Yu, Jianbin; Kolpak, Scott] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Knaus, Brian; Cronn, Richard] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Dolan, Peter] Univ Minnesota, Dept Math, Morris, MN 56267 USA. [Lorenz, W. Walter; Dean, Jeffrey F. D.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Howe, GT (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM glenn.howe@oregonstate.edu RI Dean, Jeffrey/G-2184-2010; OI Dean, Jeffrey/0000-0003-1208-1023; Cronn, Richard/0000-0001-5342-3494 FU US Department of Agriculture (USDA) National Research Initiative CSREES (Plant Genomics Coordinated Agricultural Project Award) [2007-55300-18603]; USDA National Institute of Food and Agriculture, Agriculture and Food Research Initiative (Applied Plant Genomics CAP Award) [2009-85606-05680]; Plant Genome, Genetics and Breeding Program Award [2010-65300-20166]; USDA Forest Service Rocky Mountain Research Station; US Department of Energy Joint Genome Institute (JGI) Community Sequencing Program; Pacific Northwest Tree Improvement Research Cooperative FX This work was funded by the US Department of Agriculture (USDA) National Research Initiative CSREES (Plant Genomics Coordinated Agricultural Project Award #2007-55300-18603); USDA National Institute of Food and Agriculture, Agriculture and Food Research Initiative (Applied Plant Genomics CAP Award #2009-85606-05680 and Plant Genome, Genetics and Breeding Program Award #2010-65300-20166); USDA Forest Service Rocky Mountain Research Station; US Department of Energy Joint Genome Institute (JGI) Community Sequencing Program; and the members of the Pacific Northwest Tree Improvement Research Cooperative. We thank Samuel Cushman, Barry Jaquish, Marc Rust, and Andrew Shirk for helping with the Interior Douglas-fir collections; Jim Smith, Mike Albrecht, and Joanna Warren for helping with the coastal Douglas-fir collections; Dana Howe for preparing the CBIL and YKIL RNA samples and organizing the Sanger sequencing; George Newcombe for information on the bacterial and fungal contaminants; David Neale, Gancho Slavov, Nicholas Wheeler, and Jill Wegrzyn for methodological suggestions; Randi Famula for isolating the DNA used for SNP genotyping; Vanessa Rashbrook for helping with the SNP genotyping; Alvaro Hernandez and other staff of the University of Illinois Roy J. Carver Biotechnology Center for performing 454 and Illumina sequencing; Aaron Liston and Christopher Sullivan for guidance on the use of the computing facilities at OSU's Center for Genome Research and Biocomputing; Elaine Blampied and Annie Simmonds for help with manuscript preparation; and the other co-PIs on the JGI project that produced one of the 454 datasets (Kathleen Jermstad, David Neale, and Deborah Rogers). NR 81 TC 25 Z9 25 U1 3 U2 54 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 28 PY 2013 VL 14 AR UNSP 137 DI 10.1186/1471-2164-14-137 PG 22 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 157RJ UT WOS:000319916500001 PM 23445355 ER PT J AU Hu, SJ Ning, T Fu, DY Haack, RA Zhang, Z Chen, DD Ma, XY Ye, H AF Hu, Shao-ji Ning, Tiao Fu, Da-ying Haack, Robert A. Zhang, Zhen Chen, De-dao Ma, Xue-yu Ye, Hui TI Dispersal of the Japanese Pine Sawyer, Monochamus alternatus (Coleoptera: Cerambycidae), in Mainland China as Inferred from Molecular Data and Associations to Indices of Human Activity SO PLOS ONE LA English DT Article ID TOMICUS-PINIPERDA COLEOPTERA; WILT DISEASE; MITOCHONDRIAL-DNA; HOPE COLEOPTERA; LONGHORNED BEETLE; GENETIC-STRUCTURE; SOFTWARE PACKAGE; UNITED-STATES; POPULATIONS; SCOLYTIDAE AB The Japanese pine sawyer, Monochamus alternatus Hope (Coleoptera: Cerambycidae), is an important forest pest as well as the principal vector of the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner et Buhrer), in mainland China. Despite the economic importance of this insect-disease complex, only a few studies are available on the population genetic structure of M. alternatus and the relationship between its historic dispersal pattern and various human activities. The aim of the present study was to further explore aspects of human activity on the population genetic structure of M. alternatus in mainland China. The molecular data based on the combined mitochondrial cox1 and cox2 gene fragments from 140 individuals representing 14 Chinese populations yielded 54 haplotypes. Overall, a historical (natural) expansion that originated from China's eastern coast to the western interior was revealed by the haplotype network, as well as several recent, long-distant population exchanges. Correlation analysis suggested that regional economic status and proximity to marine ports significantly influenced the population genetic structure of M. alternatus as indicated by both the ratio of shared haplotypes and the haplotype diversity, however, the PWN distribution in China was significantly correlated with only the ratio of shared haplotypes. Our results suggested that the modern logistical network (i.e., the transportation system) in China is a key medium by which humans have brought about population exchange of M. alternatus in mainland China, likely through inadvertent movement of infested wood packaging material associated with trade, and that this genetic exchange was primarily from the economically well-developed east coast of China, westward, to the less-developed interior. In addition, this study demonstrated the existence of non-local M. alternatus in new PWN-infested localities in China, but not all sites with non-local M. alternatus were infested with PWN. C1 [Hu, Shao-ji; Fu, Da-ying; Chen, De-dao; Ma, Xue-yu; Ye, Hui] Yunnan Univ, Lab Biol Invas & Ecosecur, Kunming, Peoples R China. [Hu, Shao-ji; Fu, Da-ying; Chen, De-dao; Ma, Xue-yu; Ye, Hui] Yunnan Univ, Yunnan Key Lab Int Rivers & Transboundary Ecosec, Kunming, Peoples R China. [Ning, Tiao] Yunnan Univ, Lab Conservat & Utilizat Bioresource, Minist Educ, Kunming, Peoples R China. [Ning, Tiao] Yunnan Univ, Key Lab Microbial Resources, Minist Educ, Kunming, Peoples R China. [Ning, Tiao] Yunnan Univ, Lab Anim Genet Div & Evolut Higher Educ Yunnan Pr, Kunming, Peoples R China. [Ning, Tiao] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming, Peoples R China. [Haack, Robert A.] US Forest Serv, USDA, No Res Stn, E Lansing, MI USA. [Zhang, Zhen] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China. [Zhang, Zhen] State Forestry Adm, Key Lab Forest Ecol & Environm, Beijing, Peoples R China. RP Ye, H (reprint author), Yunnan Univ, Lab Biol Invas & Ecosecur, Kunming, Peoples R China. EM yehui@ynu.edu.cn FU "Special Research Program for Non-profit Forestry" of State Forestry Administration [200904061]; "The Graduate Student Researching Program" of Yunnan Provincial Department of Education [2010J024]; "The Forestry Science and Technology Support Plan" of Chinese Academy of Forestry [2006BAD08A19105]; The International Technological Cooperation Research [2006DFA31790] FX This research was supported by "Special Research Program for Non-profit Forestry" of State Forestry Administration (200904061), "The Graduate Student Researching Program" of Yunnan Provincial Department of Education (2010J024), "The Forestry Science and Technology Support Plan" of Chinese Academy of Forestry (2006BAD08A19105), and "The International Technological Cooperation Research" (2006DFA31790). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 6 Z9 7 U1 0 U2 50 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 28 PY 2013 VL 8 IS 2 AR e57568 DI 10.1371/journal.pone.0057568 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098DA UT WOS:000315524900125 PM 23469026 ER PT J AU Shen, MQ Broeckling, CD Chu, EY Ziegler, G Baxter, IR Prenni, JE Hoekenga, OA AF Shen, Miaoqing Broeckling, Corey D. Chu, Elly Yiyi Ziegler, Gregory Baxter, Ivan R. Prenni, Jessica E. Hoekenga, Owen A. TI Leveraging Non-Targeted Metabolite Profiling via Statistical Genomics SO PLOS ONE LA English DT Article ID WIDE ASSOCIATION; MASS-SPECTROMETRY; QUANTITATIVE RESISTANCE; GENETIC ARCHITECTURE; MAPPING POPULATION; COMPLEX TRAITS; LEAF-BLIGHT; MAIZE; METABOLOMICS; LOCI AB One of the challenges of systems biology is to integrate multiple sources of data in order to build a cohesive view of the system of study. Here we describe the mass spectrometry based profiling of maize kernels, a model system for genomic studies and a cornerstone of the agroeconomy. Using a network analysis, we can include 97.5% of the 8,710 features detected from 210 varieties into a single framework. More conservatively, 47.1% of compounds detected can be organized into a network with 48 distinct modules. Eigenvalues were calculated for each module and then used as inputs for genome-wide association studies. Nineteen modules returned significant results, illustrating the genetic control of biochemical networks within the maize kernel. Our approach leverages the correlations between the genome and metabolome to mutually enhance their annotation and thus enable biological interpretation. This method is applicable to any organism with sufficient bioinformatic resources. C1 [Shen, Miaoqing] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Shen, Miaoqing; Chu, Elly Yiyi; Hoekenga, Owen A.] ARS, USDA, RW Holley Ctr Agr & Hlth, Ithaca, NY USA. [Broeckling, Corey D.; Prenni, Jessica E.] Colorado State Univ, Prote & Metabol Facil, Ft Collins, CO 80523 USA. [Ziegler, Gregory; Baxter, Ivan R.] ARS, USDA, Plant Genet Res Unit, St Louis, MO USA. [Ziegler, Gregory] Donald Danforth Plant Sci Ctr, St Louis, MO USA. RP Hoekenga, OA (reprint author), ARS, USDA, RW Holley Ctr Agr & Hlth, Ithaca, NY USA. EM Owen.Hoekenga@ars.usda.gov RI Baxter, Ivan/A-1052-2009; OI Baxter, Ivan/0000-0001-6680-1722; Prenni, Jessica/0000-0002-0337-8450 FU US Department of Agriculture's Agricultural Research Service; National Science Foundation (IOS) [1126950] FX Funding was provided to IRB and OAH by the US Department of Agriculture's Agricultural Research Service and by the National Science Foundation (IOS # 1126950). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 6 Z9 6 U1 0 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 28 PY 2013 VL 8 IS 2 AR e57667 DI 10.1371/journal.pone.0057667 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098DA UT WOS:000315524900143 PM 23469044 ER PT J AU Eiriksson, D Whitson, M Luce, CH Marshall, HP Bradford, J Benner, SG Black, T Hetrick, H McNamara, JP AF Eiriksson, David Whitson, Michael Luce, Charles H. Marshall, Hans Peter Bradford, John Benner, Shawn G. Black, Thomas Hetrick, Hank McNamara, James P. TI An evaluation of the hydrologic relevance of lateral flow in snow at hillslope and catchment scales SO HYDROLOGICAL PROCESSES LA English DT Article DE lateral flow; snow; rain on snow; snowmelt; runoff generation ID RAIN-ON-SNOW; SOIL-MOISTURE; STREAMFLOW GENERATION; MELTWATER MOVEMENT; WATER-FLOW; ICE-LAYER; USA; INFILTRATION; BALANCE; BASINS AB Lateral downslope flow in snow during snowmelt and rain-on-snow (ROS) events is a well-known phenomenon, yet its relevance to water redistribution at hillslope and catchment scales is not well understood. We used dye tracers, geophysical methods, and hydrometric measurements to describe the snow properties that promote lateral flow, assess the relative velocities of lateral flow in snow and soil, and estimate volumes of downslope flow. Results demonstrate that rain and melt water can travel tens of metres downslope along layers within the snowpack or at the snowpack base within tens of hours. Lateral flow within the snowpack becomes less likely as the snowpack becomes saturated and stratigraphic boundaries are destroyed. Flow along the base can be prevalent in all snowpack conditions. The net result of lateral flow in snow can be the deposition of water on the soil surface in advanced downslope positions relative to its point of origin, or direct discharge to a stream. Although both melt and ROS events can redistribute water to downslope positions, ROS events produced the most significant volumes of downslope flow. Direct stream contributions through the snowpack during one ROS event produced up to 12% of streamflow during the event. This can help explain rapid delivery of water to streams during ROS events, as well as anomalously high contributions of event water during snowmelt hydrographs. In catchments with a persistent snowpack, lateral redistribution of water within the snowpack should be considered a relevant moisture redistribution mechanism. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Eiriksson, David; Whitson, Michael; Marshall, Hans Peter; Bradford, John; Benner, Shawn G.; Hetrick, Hank; McNamara, James P.] Boise State Univ, Dept Geosci, Boise, ID 83725 USA. [Luce, Charles H.; Black, Thomas] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID USA. RP McNamara, JP (reprint author), Boise State Univ, Dept Geosci, Boise, ID 83725 USA. EM jmcnamar@boisestate.edu RI Benner, Shawn/A-2557-2012; McNamara, James/F-1993-2011; Luce, Charles/A-9267-2008 OI Benner, Shawn/0000-0003-4024-5777; Luce, Charles/0000-0002-6938-9662 FU National Science Foundation [CBET-0854522, EAR-0943710]; National Atmospheric and Oceanic Administration [NA08NWS4620047] FX This research was supported in part by grants from the National Science Foundation (awards CBET-0854522 and EAR-0943710) and the National Atmospheric and Oceanic Administration (award NA08NWS4620047). NR 52 TC 18 Z9 18 U1 1 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 28 PY 2013 VL 27 IS 5 SI SI BP 640 EP 654 DI 10.1002/hyp.9666 PG 15 WC Water Resources SC Water Resources GA 095UE UT WOS:000315359400003 ER PT J AU Safeeq, M Grant, GE Lewis, SL Tague, CL AF Safeeq, Mohammad Grant, Gordon E. Lewis, Sarah L. Tague, Christina. L. TI Coupling snowpack and groundwater dynamics to interpret historical streamflow trends in the western United States SO HYDROLOGICAL PROCESSES LA English DT Article DE streamflow trend; hydrologic processes; groundwater processes; climate; warming ID ESTIMATION EXPERIMENT MOPEX; RECESSION ANALYSIS; CLIMATE-CHANGE; PARAMETER-ESTIMATION; NORTH-AMERICA; FLOW REGIMES; TEMPERATURE; HYDROLOGY; PRECIPITATION; SENSITIVITY AB A key challenge for resource and land managers is predicting the consequences of climate warming on streamflow and water resources. During the last century in the western United States, significant reductions in snowpack and earlier snowmelt have led to an increase in the fraction of annual streamflow during winter and a decline in the summer. Previous work has identified elevation as it relates to snowpack dynamics as the primary control on streamflow sensitivity to warming. But along with changes in the timing of snowpack accumulation and melt, summer streamflows are also sensitive to intrinsic, geologically mediated differences in the efficiency of landscapes in transforming recharge (either as rain or snow) into discharge; we term this latter factor drainage efficiency. Here we explore the conjunction of drainage efficiency and snowpack dynamics in interpreting retrospective trends in summer streamflow during 19502010 using daily streamflow from 81 watersheds across the western United States. The recession constant (k) and fraction of precipitation falling as snow (Sf) were used as metrics of deep groundwater and overall precipitation regime (rain and/or snow), respectively. This conjunctive analysis indicates that summer streamflows in watersheds that drain slowly from deep groundwater and receive precipitation as snow are most sensitive to climate warming. During the spring, however, watersheds that drain rapidly and receive precipitation as snow are most sensitive to climate warming. Our results indicate that not all trends in western United States are associated with changes in snowpack dynamics; we observe declining streamflow in late fall and winter in rain-dominated watersheds as well. These empirical findings support both theory and hydrologic modelling and have implications for how streamflow sensitivity to warming is interpreted across broad regions. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Safeeq, Mohammad; Lewis, Sarah L.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Grant, Gordon E.] US Forest Serv, PNW Res Stn, USDA, Corvallis, OR 97331 USA. [Tague, Christina. L.] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. RP Grant, GE (reprint author), US Forest Serv, PNW Res Stn, USDA, Corvallis, OR 97331 USA. EM ggrant@fs.fed.us FU Oregon Watershed Enhancement Board; Bureau of Land Management (Oregon); USDA Forest Service Region 6; Pacific Northwest Research Station FX The authors gratefully thank two anonymous manuscript reviewers for their helpful comments. They acknowledge funding support from the Oregon Watershed Enhancement Board, the Bureau of Land Management (Oregon) and the USDA Forest Service Region 6 and Pacific Northwest Research Station. NR 45 TC 20 Z9 20 U1 5 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 28 PY 2013 VL 27 IS 5 SI SI BP 655 EP 668 DI 10.1002/hyp.9628 PG 14 WC Water Resources SC Water Resources GA 095UE UT WOS:000315359400004 ER PT J AU Goode, JR Buffington, JM Tonina, D Isaak, DJ Thurow, RF Wenger, S Nagel, D Luce, C Tetzlaff, D Soulsby, C AF Goode, Jaime R. Buffington, John M. Tonina, Daniele Isaak, Daniel J. Thurow, Russell F. Wenger, Seth Nagel, David Luce, Charlie Tetzlaff, Doerthe Soulsby, Chris TI Potential effects of climate change on streambed scour and risks to salmonid survival in snow-dominated mountain basins SO HYDROLOGICAL PROCESSES LA English DT Article DE climate change; morphologic adjustment; salmon spawning habitat; streambed scour ID WESTERN UNITED-STATES; COLUMBIA RIVER-BASIN; EGG BURIAL DEPTHS; CHINOOK SALMON; PACIFIC SALMON; FINE-SEDIMENT; EVOLUTIONARY HISTORY; HYDRAULIC ROUGHNESS; TEMPORAL VARIATION; CHANNEL-TYPE AB Snowmelt-dominated basins in northern latitudes provide critical habitat for salmonids. As such, these systems may be especially vulnerable to climate change because of potential shifts in the frequency, magnitude, and timing of flows that can scour incubating embryos. A general framework is presented to examine this issue, using a series of physical models that link climate change, streamflow, and channel morphology to predict the magnitude and spatial distribution of streambed scour and consequent risk to salmonid embryos at basin scales. The approach is demonstrated for a mountain catchment in the Northern Rocky Mountains, USA. Results show that risk of critical scour varies as a function of species and life history and is modulated by local variations in lithology and channel confinement. Embryos of smaller-bodied fall spawners may be at greater risk because of shallow egg burial depths and increased rain-on-snow events during their incubation period. Scour risk for all species is reduced when changes in channel morphology (width, depth, and grain size) keep pace with climate-driven changes in streamflow. Although climate change is predicted to increase scour magnitude, the frequency of scouring events relative to typical salmonid life cycles is relatively low, indicating that individual year classes may be impacted by critical scour, but extirpation of entire populations is not expected. Furthermore, refugia are predicted to occur in unconfined portions of the stream network, where scouring shear stresses are limited to bankfull stage because overbank flows spread across alluvial floodplains; conversely, confined valleys will likely exacerbate climate-driven changes in flow and scour. Our approach can be used to prioritize management strategies according to relative risk to different species or spatial distributions of risk and can be used to predict temporal shifts in the spatial distribution of suitable spawning habitats. A critical unknown issue is whether biological adaptation can keep pace with rates of climate change and channel response. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Goode, Jaime R.; Tonina, Daniele] Univ Idaho, Ctr Ecohydraul Res, Boise, ID 83702 USA. [Goode, Jaime R.; Buffington, John M.; Isaak, Daniel J.; Thurow, Russell F.; Nagel, David; Luce, Charlie] US Forest Serv, Rocky Mt Res Stn, Boise, ID USA. [Wenger, Seth] Trout Unltd, Boise, ID USA. [Tetzlaff, Doerthe; Soulsby, Chris] Univ Aberdeen, Sch Geosci, Northern Rivers Inst, Aberdeen AB9 1FX, Scotland. RP Goode, JR (reprint author), Univ Idaho, Ctr Ecohydraul Res, USFS, Rocky Mt Res Stn, Boise, ID 83702 USA. EM jaimeg@uidaho.edu RI Tonina, Daniele/I-7688-2012; Luce, Charles/A-9267-2008; OI Tonina, Daniele/0000-0002-1866-1013; Luce, Charles/0000-0002-6938-9662; Tetzlaff, Doerthe/0000-0002-7183-8674 FU USDA Forest Service [09-JV-11221634-314] FX This work was funded by USDA Forest Service Grant 09-JV-11221634-314. We very much appreciate the thorough and insightful comments provided by two anonymous reviewers. NR 93 TC 28 Z9 28 U1 0 U2 56 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 28 PY 2013 VL 27 IS 5 SI SI BP 750 EP 765 DI 10.1002/hyp.9728 PG 16 WC Water Resources SC Water Resources GA 095UE UT WOS:000315359400012 ER PT J AU Liu, Y Huo, NX Dong, LL Wang, Y Zhang, SX Young, HA Feng, XX Gu, YQ AF Liu, Yue Huo, Naxin Dong, Lingli Wang, Yi Zhang, Shuixian Young, Hugh A. Feng, Xiaoxiao Gu, Yong Qiang TI Complete Chloroplast Genome Sequences of Mongolia Medicine Artemisia frigida and Phylogenetic Relationships with Other Plants SO PLOS ONE LA English DT Article ID SUNFLOWER FAMILY ASTERACEAE; ANCIENT EVOLUTIONARY SPLIT; PLASTID GENOME; DNA INVERSION; ARABIDOPSIS-THALIANA; MOLECULAR PHYLOGENY; HORDEUM-VULGARE; MAJOR CLADES; LAND PLANTS; REPEATS AB Background: Artemisia frigida Willd. is an important Mongolian traditional medicinal plant with pharmacological functions of stanch and detumescence. However, there is little sequence and genomic information available for Artemisia frigida, which makes phylogenetic identification, evolutionary studies, and genetic improvement of its value very difficult. We report the complete chloroplast genome sequence of Artemisia frigida based on 454 pyrosequencing. Methodology/Principal Findings: The complete chloroplast genome of Artemisia frigida is 151,076 bp including a large single copy (LSC) region of 82,740 bp, a small single copy (SSC) region of 18,394 bp and a pair of inverted repeats (IRs) of 24,971 bp. The genome contains 114 unique genes and 18 duplicated genes. The chloroplast genome of Artemisia frigida contains a small 3.4 kb inversion within a large 23 kb inversion in the LSC region, a unique feature in Asteraceae. The gene order in the SSC region of Artemisia frigida is inverted compared with the other 6 Asteraceae species with the chloroplast genomes sequenced. This inversion is likely caused by an intramolecular recombination event only occurred in Artemisia frigida. The existence of rich SSR loci in the Artemisia frigida chloroplast genome provides a rare opportunity to study population genetics of this Mongolian medicinal plant. Phylogenetic analysis demonstrates a sister relationship between Artemisia frigida and four other species in Asteraceae, including Ageratina adenophora, Helianthus annuus, Guizotia abyssinica and Lactuca sativa, based on 61 protein-coding sequences. Furthermore, Artemisia frigida was placed in the tribe Anthemideae in the subfamily Asteroideae (Asteraceae) based on ndhF and trnL-F sequence comparisons. Conclusion: The chloroplast genome sequence of Artemisia frigida was assembled and analyzed in this study, representing the first plastid genome sequenced in the Anthemideae tribe. This complete chloroplast genome sequence will be useful for molecular ecology and molecular phylogeny studies within Artemisia species and also within the Asteraceae family. C1 [Liu, Yue; Zhang, Shuixian; Feng, Xiaoxiao] Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China. [Liu, Yue] China Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing, Peoples R China. [Liu, Yue; Huo, Naxin; Dong, Lingli; Wang, Yi; Young, Hugh A.; Gu, Yong Qiang] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Liu, Yue] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Zhang, Shuixian; Feng, Xiaoxiao] Minzu Univ China, Grad Sch, Beijing, Peoples R China. RP Liu, Y (reprint author), Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China. EM yueliu@caas.net.cn; yong.gu@ars.usda.gov FU National Science Foundation of China [30801554, 81110108011]; China Postdoctoral Science Foundation [20110490556]; 111 Project [B08044]; 985 Project of Minzu University of China [MUC98504-14, MUC98507-08]; 211 Project of Minzu University of China FX This work was supported by National Science Foundation of China (30801554 and 81110108011), China Postdoctoral Science Foundation (20110490556), 111 Project (B08044), 985 Project of Minzu University of China (MUC98504-14 and MUC98507-08), and 211 Project of Minzu University of China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 85 TC 11 Z9 14 U1 2 U2 50 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 27 PY 2013 VL 8 IS 2 AR e57533 DI 10.1371/journal.pone.0057533 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098BN UT WOS:000315519000126 PM 23460871 ER PT J AU Mettke-Hofmann, C Winkler, H Hamel, PB Greenberg, R AF Mettke-Hofmann, Claudia Winkler, Hans Hamel, Paul B. Greenberg, Russell TI Migratory New World Blackbirds (Icterids) Are More Neophobic than Closely Related Resident Icterids SO PLOS ONE LA English DT Article ID ECOLOGICAL PLASTICITY; PUBLIC INFORMATION; NOVELTY RESPONSES; SWAMP SPARROWS; BIRDS; EXPERIENCE; ASSEMBLAGE; FOOD; SONG; EXPLORATION AB Environments undergo short-term and long-term changes due to natural or human-induced events. Animals differ in their ability to cope with such changes which can be related to their ecology. Changes in the environment often elicit avoidance reactions (neophobia) which protect animals from dangerous situations but can also inhibit exploration and familiarization with novel situations and thus, learning about new resources. Studies investigating the relationship between a species' ecology and its neophobia have so far been restricted to comparing only a few species and mainly in captivity. The current study investigated neophobia reactions to experimentally-induced changes in the natural environment of six closely-related blackbird species (Icteridae), including two species represented by two distinct populations. For analyses, neophobic reactions (difference in number of birds feeding and time spent feeding with and without novel objects) were related to several measures of ecological plasticity and the migratory strategy (resident or migratory) of the population. Phylogenetic relationships were incorporated into the analysis. The degree of neophobia was related to migratory strategy with migrants expressing much higher neophobia (fewer birds feeding and for a shorter time with objects present) than residents. Furthermore, neophobia showed a relationship to diet breadth with fewer individuals of diet generalists than specialists returning when objects were present supporting the dangerous niche hypothesis. Residents may have evolved lower neophobia as costs of missing out on opportunities may be higher for residents than migrants as the former are restricted to a smaller area. Lower neophobia allows them approaching changes in the environment (e. g. novel objects) quickly, thereby securing access to resources. Additionally, residents have a greater familiarity with similar situations in the area than migrants and the latter may, therefore, initially stay behind resident species. C1 [Mettke-Hofmann, Claudia] Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 5UX, Merseyside, England. [Mettke-Hofmann, Claudia; Greenberg, Russell] Smithsonian Inst, Natl Zool Pk, Smithsonian Migratory Bird Ctr, Washington, DC 20008 USA. [Winkler, Hans] Univ Vet Med, Dept Integrat Biol & Evolut, Konrad Lorenz Inst Ethol, Vienna, Austria. [Hamel, Paul B.] US Forest Serv, Southern Hardwood Lab, Stoneville, MS USA. RP Mettke-Hofmann, C (reprint author), Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 5UX, Merseyside, England. EM C.C.Mettke-Hofmann@ljmu.ac.uk FU Max Kade Foundation; Max Planck Institute for Ornithology, Andechs, Germany; German Ethological Society; Society for Tropical Ornithology, Germany; Point Reyes Bird Observatory, CA; USDA Forest Service, Center for Bottomland Hardwoods, MS FX The study was supported by a grant from the Max Kade Foundation. Assistants, travel and material were supported by the Max Planck Institute for Ornithology, Andechs, Germany, the German Ethological Society, and the Society for Tropical Ornithology, Germany. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank the Point Reyes Bird Observatory, CA, and the USDA Forest Service, Center for Bottomland Hardwoods, MS, for providing housing and support, the National Park Service Point Reyes National Seashore, CA, Yazoo NWR, Leroy Percy SP, Delta Research and Experimental Center, (all MS), and all cooperating farmers for permitting experiments on their land and Gerhard Hofmann for his assistance with the experiments. NR 53 TC 13 Z9 13 U1 4 U2 52 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 27 PY 2013 VL 8 IS 2 AR e57565 DI 10.1371/journal.pone.0057565 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098BN UT WOS:000315519000130 PM 23460875 ER PT J AU Moustafa, ME Carlson, BA Anver, MR Bobe, G Zhong, NX Ward, JM Perella, CM Hoffmann, VJ Rogers, K Combs, GF Schweizer, U Merlino, G Gladyshev, VN Hatfield, DL AF Moustafa, Mohamed E. Carlson, Bradley A. Anver, Miriam R. Bobe, Gerd Zhong, Nianxin Ward, Jerrold M. Perella, Christine M. Hoffmann, Victoria J. Rogers, Keith Combs, Gerald F., Jr. Schweizer, Ulrich Merlino, Glenn Gladyshev, Vadim N. Hatfield, Dolph L. TI Selenium and Selenoprotein Deficiencies Induce Widespread Pyogranuloma Formation in Mice, while High Levels of Dietary Selenium Decrease Liver Tumor Size Driven by TGF alpha SO PLOS ONE LA English DT Article ID SELENOCYSTEINE TRANSFER-RNA; TRANSGENIC MICE; MAMMALIAN-CELLS; EXPRESSION; MOUSE; CANCER; HEPATOCARCINOGENESIS; INFLAMMATION; POPULATION; DELETION AB Changes in dietary selenium and selenoprotein status may influence both anti- and pro-cancer pathways, making the outcome of interventions different from one study to another. To characterize such outcomes in a defined setting, we undertook a controlled hepatocarcinogenesis study involving varying levels of dietary selenium and altered selenoprotein status using mice carrying a mutant (A37G) selenocysteine tRNA transgene (Trsp(tG37)) and/or a cancer driver TGF alpha transgene. The use of Trsp(tG37) altered selenoprotein expression in a selenoprotein and tissue specific manner and, at sufficient dietary selenium levels, separate the effect of diet and selenoprotein status. Mice were maintained on diets deficient in selenium (0.02 ppm selenium) or supplemented with 0.1, 0.4 or 2.25 ppm selenium or 30 ppm triphenylselenonium chloride (TPSC), a non-metabolized selenium compound. Trsp(tG37) transgenic and TGF alpha/Trsp(tG37) bi-transgenic mice subjected to selenium-deficient or TPSC diets developed a neurological phenotype associated with early morbidity and mortality prior to hepatocarcinoma development. Pathology analyses revealed widespread disseminated pyogranulomatous inflammation. Pyogranulomas occurred in liver, lungs, heart, spleen, small and large intestine, and mesenteric lymph nodes in these transgenic and bi-transgenic mice. The incidence of liver tumors was significantly increased in mice carrying the TGF alpha transgene, while dietary selenium and selenoprotein status did not affect tumor number and multiplicity. However, adenoma and carcinoma size and area were smaller in TGF alpha transgenic mice that were fed 0.4 and 2.25 versus 0.1 ppm of selenium. Thus, selenium and selenoprotein deficiencies led to widespread pyogranuloma formation, while high selenium levels inhibited the size of TGF alpha-induced liver tumors. C1 [Moustafa, Mohamed E.; Carlson, Bradley A.; Zhong, Nianxin; Hatfield, Dolph L.] NCI, Mol Biol Selenium Sect, Lab Canc Prevent, NIH, Bethesda, MD 20892 USA. [Anver, Miriam R.; Rogers, Keith] Sci Applicat Int Corp Frederick Inc, Pathol Histotechnol Lab, Frederick Natl Lab Canc Res, Frederick, MD USA. [Bobe, Gerd] Oregon State Univ, Dept Anim & Rangeland Sci, Coll Agr, Corvallis, OR 97331 USA. [Bobe, Gerd] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. [Ward, Jerrold M.] NIAID, Immunopathol Sect, NIH, Bethesda, MD 20892 USA. [Perella, Christine M.] Sci Applicat Int Corp Frederick Inc, Lab Anim Sci Program, Frederick Natl Lab Canc Res, Frederick, MD USA. [Hoffmann, Victoria J.] NCI, Off Director, Diagnost & Res Serv Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. [Combs, Gerald F., Jr.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Schweizer, Ulrich] Charite, Inst Expt Endokrinol, D-13353 Berlin, Germany. [Merlino, Glenn] NCI, Lab Canc Biol & Genet, NIH, Bethesda, MD 20892 USA. [Gladyshev, Vadim N.] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA. [Gladyshev, Vadim N.] Harvard Univ, Sch Med, Boston, MA USA. RP Hatfield, DL (reprint author), NCI, Mol Biol Selenium Sect, Lab Canc Prevent, NIH, Bethesda, MD 20892 USA. EM hatfield@dc37a.nci.nih.gov RI Gladyshev, Vadim/A-9894-2013; Schweizer, Ulrich/E-8105-2013 FU Intramural Research Program of the National Institutes of Health (NIH); National Cancer Institute; Center for Cancer Research; NIH; National Cancer Institute, National Institutes of Health [HHSN261200800001E] FX This work was supported by the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute, Center for Cancer Research to DLH, NIH grants to VNG and the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The authors express their sincere appreciation to BAC, R. Irons and C. D. Davis who made the video shown in the Online Supplementary Information in DLH's laboratory. The content of this publication does not necessarily reflect the views of policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 11 Z9 11 U1 0 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 27 PY 2013 VL 8 IS 2 AR e57389 DI 10.1371/journal.pone.0057389 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098BN UT WOS:000315519000101 PM 23460847 ER PT J AU Oakley, BB Morales, CA Line, J Berrang, ME Meinersmann, RJ Tillman, GE Wise, MG Siragusa, GR Hiett, KL Seal, BS AF Oakley, Brian B. Morales, Cesar A. Line, J. Berrang, Mark E. Meinersmann, Richard J. Tillman, Glenn E. Wise, Mark G. Siragusa, Gregory R. Hiett, Kelli L. Seal, Bruce S. TI The Poultry-Associated Microbiome: Network Analysis and Farm-to-Fork Characterizations SO PLOS ONE LA English DT Article ID INTESTINAL BACTERIAL COMMUNITY; REAL-TIME PCR; CAMPYLOBACTER-JEJUNI; BROILER-CHICKENS; FOODBORNE ILLNESS; RARE BIOSPHERE; UNITED-STATES; FAECALIBACTERIUM-PRAUSNITZII; PATHOGEN CAMPYLOBACTER; COMPETITIVE-EXCLUSION AB Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS), quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of longitudinal data following two flocks from the farm through processing showed a core microbiome containing multiple sequence types most closely related to genera known to be pathogenic for animals and/or humans, including Campylobacter, Clostridium, and Shigella. After the final stage of commercial poultry processing, taxonomic richness was ca. 2-4 times lower than the richness of fecal samples from the same flocks and Campylobacter abundance was significantly reduced. Interestingly, however, carcasses sampled at 48 hr after processing harboured the greatest proportion of unique taxa (those not encountered in other samples), significantly more than expected by chance. Among these were anaerobes such as Prevotella, Veillonella, Leptrotrichia, and multiple Campylobacter sequence types. Retail products were dominated by Pseudomonas, but also contained 27 other genera, most of which were potentially metabolically active and encountered in on-farm samples. Network analysis was focused on the foodborne pathogen Campylobacter and revealed a majority of sequence types with no significant interactions with other taxa, perhaps explaining the limited efficacy of previous attempts at competitive exclusion of Campylobacter. These data represent the first use of HTS to characterize the poultry microbiome across a series of farm-to-fork samples and demonstrate the utility of HTS in monitoring the food supply chain and identifying sources of potential zoonoses and interactions among taxa in complex communities. C1 [Oakley, Brian B.; Morales, Cesar A.; Line, J.; Tillman, Glenn E.; Wise, Mark G.; Siragusa, Gregory R.; Hiett, Kelli L.; Seal, Bruce S.] ARS, USDA, Richard B Russell Agr Res Ctr, Athens, GA USA. [Berrang, Mark E.; Meinersmann, Richard J.] ARS, Bacterial Epidemiol & Antibiot Resistance Res Uni, USDA, Richard B Russell Agr Res Ctr, Athens, GA USA. RP Oakley, BB (reprint author), ARS, USDA, Richard B Russell Agr Res Ctr, Athens, GA USA. EM brian.oakley@ars.usda.gov FU USDA ARS CRIS [6612-32000-059, 6612-32000-060, 6612-41420-004] FX Funding was provided by the USDA ARS CRIS project 6612-32000-059, Molecular Characterization and Gastrointestinal Tract Ecology of Commensal Human Food-Borne Bacterial Pathogens in the Chicken, 6612-32000-060, Interventions and Methodologies to Reduce Human Food-Borne Bacterial? Pathogens in Chickens, and 6612-41420-004, Microbial Ecology of Human Pathogens Relative to Poultry Processing. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 83 TC 20 Z9 20 U1 4 U2 63 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 27 PY 2013 VL 8 IS 2 AR e57190 DI 10.1371/journal.pone.0057190 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098BN UT WOS:000315519000073 PM 23468931 ER PT J AU Olson, DH Aanensen, DM Ronnenberg, KL Powell, CI Walker, SF Bielby, J Garner, TWJ Weaver, G Fisher, MC AF Olson, Deanna H. Aanensen, David M. Ronnenberg, Kathryn L. Powell, Christopher I. Walker, Susan F. Bielby, Jon Garner, Trenton W. J. Weaver, George Fisher, Matthew C. CA Bd Mapping Grp TI Mapping the Global Emergence of Batrachochytrium dendrobatidis, the Amphibian Chytrid Fungus SO PLOS ONE LA English DT Article ID EMERGING INFECTIOUS-DISEASE; RANA-CATESBEIANA; CHYTRIDIOMYCOSIS; PATHOGEN; DECLINES; EXTINCTION; COMMUNITY; FROGS; SUSCEPTIBILITY; EPIDEMIOLOGY AB The rapid worldwide emergence of the amphibian pathogen Batrachochytrium dendrobatidis (Bd) is having a profound negative impact on biodiversity. However, global research efforts are fragmented and an overarching synthesis of global infection data is lacking. Here, we provide results from a community tool for the compilation of worldwide Bd presence and report on the analyses of data collated over a four-year period. Using this online database, we analysed: 1) spatial and taxonomic patterns of infection, including amphibian families that appear over-and under-infected; 2) relationships between Bd occurrence and declining amphibian species, including associations among Bd occurrence, species richness, and enigmatic population declines; and 3) patterns of environmental correlates with Bd, including climate metrics for all species combined and three families (Hylidae, Bufonidae, Ranidae) separately, at both a global scale and regional (U. S. A.) scale. These associations provide new insights for downscaled hypothesis testing. The pathogen has been detected in 52 of 82 countries in which sampling was reported, and it has been detected in 516 of 1240 (42%) amphibian species. We show that detected Bd infections are related to amphibian biodiversity and locations experiencing rapid enigmatic declines, supporting the hypothesis that greater complexity of amphibian communities increases the likelihood of emergence of infection and transmission of Bd. Using a global model including all sampled species, the odds of Bd detection decreased with increasing temperature range at a site. Further consideration of temperature range, rather than maximum or minimum temperatures, may provide new insights into Bd-host ecology. Whereas caution is necessary when interpreting such a broad global dataset, the use of our pathogen database is helping to inform studies of the epidemiology of Bd, as well as enabling regional, national, and international prioritization of conservation efforts. We provide recommendations for adaptive management to enhance the database utility and relevance. C1 [Olson, Deanna H.; Ronnenberg, Kathryn L.; Weaver, George] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Aanensen, David M.; Powell, Christopher I.; Walker, Susan F.; Fisher, Matthew C.] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London, England. [Bielby, Jon] Zool Soc London, Inst Zool, London NW1 4RY, England. [Garner, Trenton W. J.] Oregon State Univ, Dept Stat, Corvallis, OR 97331 USA. RP Olson, DH (reprint author), US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM dedeolson@fs.fed.us RI Fisher, Matthew/B-9094-2011; Cunningham, Andrew/E-7536-2010; Garner, Trenton/D-6873-2011; OI Fisher, Matthew/0000-0002-1862-6402 FU UK Natural Environmental Research Council [E006701/1]; ERAnet BIODIVERSA project RACE; Wellcome Trust [WT08972]; US Forest Service, Pacific Northwest Research Station FX Funds were provided by the UK Natural Environmental Research Council (E006701/1), The ERAnet BIODIVERSA project RACE, The Wellcome Trust (WT08972), and the US Forest Service, Pacific Northwest Research Station. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 61 TC 95 Z9 107 U1 13 U2 268 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 27 PY 2013 VL 8 IS 2 AR e56802 DI 10.1371/journal.pone.0056802 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098BN UT WOS:000315519000039 PM 23463502 ER PT J AU Sobolev, VS AF Sobolev, Victor S. TI Production of Phytoalexins in Peanut (Arachis hypogaea) Seed Elicited by Selected Microorganisms SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE peanut; Arachis hypogaea; Leguminosae; groundnut; phytoalexin; stilbenoid; biotic elicitor; Aspergillus; Bacillus; Rhizobium; Cladosporium; Saccharomyces; Candida ID STILBENE PHYTOALEXINS; ASPERGILLUS-CAELATUS; RESVERATROL; KERNELS; INTERRELATIONSHIP; BIOSYNTHESIS; PURIFICATION; ANTIOXIDANT; TEMPERATURE; METABOLISM AB Under favorable conditions, the peanut plant demonstrates appreciable resistance to fungal invasion by producing and accumulating phytoalexins, antimicrobial stilbenoids. This mechanism for resistance is little understood, yet it is crucial for breeding and genetically modifying peanut plants to develop new cultivars with fungal resistance. The dynamics of phytoalexin production in peanut seeds and embryos challenged by selected important fungi and bacteria was investigated. Different biotic agents selectively elicited production of major peanut stilbenoids, resveratrol, arachidin-1, arachidin-3, and SB-1. Aspergillis species, compared to other biotic agents, were more potent elicitors of stilbenoids. Embryos demonstrated significantly higher production of stilbenoids compared to cotyledons and may serve as a convenient source of genetic material in isolating genes for peanut plant defense enhancement. C1 ARS, Natl Peanut Res Lab, USDA, Dawson, GA 39842 USA. RP Sobolev, VS (reprint author), ARS, Natl Peanut Res Lab, USDA, POB 509, Dawson, GA 39842 USA. EM vsobolev@nprl.usda.gov NR 40 TC 9 Z9 9 U1 2 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 27 PY 2013 VL 61 IS 8 BP 1850 EP 1858 DI 10.1021/jf3054752 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 099KD UT WOS:000315619000030 PM 23387286 ER PT J AU Liu, ZS Biswas, A AF Liu, Zengshe Biswas, Atanu TI Fluoroantimonic acid hexahydrate (HSbF6 center dot 6H(2)O) catalysis: The ring-opening polymerization of epoxidized soybean oil SO APPLIED CATALYSIS A-GENERAL LA English DT Article DE Epoxidized soybean oil; Ring-opening polymerization; Fluoroantimonic acid hexahydrate ID RENEWABLE RESOURCES; METHYL OLEATE; POLYMERS; POLYURETHANES; POLYOLS AB Ring-opening polymerization of epoxidized soybean oil (ESO) catalyzed by a super acid, fluoroantimonic acid hexahydrate (HSbF6 center dot 6H(2)O) in ethyl acetate was conducted in an effort to develop useful biodegradable polymers. The resulting polymerized ESO (SA-RPESO) was characterized using infrared (IR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), H-1 NMR, C-13 NMR, solid state 13 degrees C NMR, and gel permeation chromatography (GPC). The results indicated that ESO was effectively polymerized by fluoroantimonic acid and formed polymers with relatively high crosslink density. Glass transition temperatures of these polymers ranged from 13 degrees C to 21 degrees C. TGA results showed the SA-RPESO polymers were thermally stable at temperatures up to 200 degrees C. Decomposition of the polymers was found to occur at temperatures greater than 350 degrees C. GPC results indicated the extracted soluble substances from SA-RPESO polymers were oligomers of ESO. These soybean oil-based polymers will be functionalized to hydrogels and their applications explored in the personal and health care areas. Published by Elsevier B.V. C1 [Liu, Zengshe; Biswas, Atanu] USDA ARS, NCAUR, Peoria, IL 61604 USA. RP Liu, ZS (reprint author), USDA ARS, NCAUR, 1815N Univ St, Peoria, IL 61604 USA. EM kevin.liu@ars.usda.gov NR 21 TC 6 Z9 6 U1 0 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-860X J9 APPL CATAL A-GEN JI Appl. Catal. A-Gen. PD FEB 26 PY 2013 VL 453 BP 370 EP 375 DI 10.1016/j.apcata.2012.12.028 PG 6 WC Chemistry, Physical; Environmental Sciences SC Chemistry; Environmental Sciences & Ecology GA 099GU UT WOS:000315610300044 ER PT J AU Smigocki, AC Ivic-Haymes, S Li, HY Savic, J AF Smigocki, Ann C. Ivic-Haymes, Snezana Li, Haiyan Savic, Jelena TI Pest Protection Conferred by a Beta vulgaris Serine Proteinase Inhibitor Gene SO PLOS ONE LA English DT Article ID COLORADO POTATO BEETLE; PLANT PROTEASE INHIBITORS; ORYZACYSTATIN-I; TRANSGENIC TOBACCO; SUGAR-BEET; HELICOVERPA-ARMIGERA; CYSTEINE PROTEINASE; INSECT-RESISTANT; HAIRY ROOTS; INSENSITIVE PROTEASES AB Proteinase inhibitors provide a means of engineering plant resistance to insect pests. A Beta vulgaris serine proteinase inhibitor gene (BvSTI) was fused to the constitutive CaMV35S promoter for over-expression in Nicotiana benthamiana plants to study its effect on lepidopteran insect pests. Independently derived BvSTI transgenic tobacco T2 homozygous progeny were shown to have relatively high BvSTI gene transcript levels. BvSTI-specific polyclonal antibodies cross-reacted with the expected 30 kDA recombinant BvSTI protein on Western blots. In gel trypsin inhibitor activity assays revealed a major clear zone that corresponded to the BvSTI proteinase inhibitor that was not detected in the untransformed control plants. BvSTI-transgenic plants were bioassayed for resistance to five lepidopteran insect pests. Spodoptera frugiperda, S. exigua and Manduca sexta larvae fed BvSTI leaves had significant reductions in larval weights as compared to larvae fed on untransformed leaves. In contrast, larval weights increased relative to the controls when Heliothis virescens and Agrotis ipsilon larvae were fed on BvSTI leaves. As the larvae entered the pupal stage, pupal sizes reflected the overall larval weights. Some developmental abnormalities of the pupae and emerging moths were noted. These findings suggest that the sugar beet BvSTI gene may prove useful for effective control of several different lepidopteran insect pests in genetically modified tobacco and other plants. The sugar beet serine proteinase inhibitor may be more effective for insect control because sugar beet is cropped in restricted geographical areas thus limiting the exposure of the insects to sugar beet proteinase inhibitors and build up of non-sensitive midgut proteases. C1 [Smigocki, Ann C.; Ivic-Haymes, Snezana; Li, Haiyan; Savic, Jelena] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD USA. RP Smigocki, AC (reprint author), ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD USA. EM Ann.Smigocki@ars.usda.gov FU congress FX This is a US government facility and the funding is allocated by congress for the type of research being reported in this manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript NR 58 TC 9 Z9 9 U1 0 U2 39 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 26 PY 2013 VL 8 IS 2 AR e57303 DI 10.1371/journal.pone.0057303 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 098PO UT WOS:000315561400037 PM 23468963 ER PT J AU Gonnella, TP Keating, JM Kjemhus, JA Picklo, MJ Biggane, JP AF Gonnella, Thomas P. Keating, Jennifer M. Kjemhus, Jessica A. Picklo, Matthew J. Biggane, Joseph P. TI Fluorescence lifetime analysis and effect of magnesium ions on binding of NADH to human aldehyde dehydrogenase 1 SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE Aldehyde dehydrogenase; Fluorescence; NADH ID INTRACELLULAR NADH; ISOMERIZATION; CONFORMATION; INHIBITION; CATALYSIS; ENZYME AB Aldehyde dehydrogenase 1 (ALDH1A1) catalyzes the oxidation of toxic aldehydes to carboxylic acids. Physiologic levels of Mg2+ ions decrease ALDH1 activity in part by increasing NADH binding affinity to the enzyme. By using time-resolved fluorescence spectroscopy, we have resolved the fluorescent lifetimes (tau) of free NADH in solution (tau = 0.4 ns) and two enzyme-bound NADH states (tau = 2.0 ns and tau = 7.7 ns). We used this technique to investigate the effects of Mg2+ ions on the ALDH1A1-NADH binding characteristics and enzyme catalysis. From the resolved free and bound NADH fluorescence signatures, the K-D values for both NADH conformations in ALDH1A1 ranged from about 24 mu M to 1 mu M for Mg2+ ion concentrations of 0-6000 mu M, respectively. The rate constants for dissociation of the enzyme-NADH complex ranged from 0.03 s(-1) (6000 mu M Mg2+) to 030 s(-1) (0 mu M Mg2+) as determined by addition of excess NAD(+) to prevent re-association of NADH and resolving the real-time NADH fluorescence signal. During the initial reaction of enzyme with NAD(+) and butyraldehyde, there was an immediate rise in the NADH fluorescence, due to the formation of bound NADH complexes, with a constant steady-state rate of production of free NADH. As the Mg2+ ion concentration was increased, there was a consistent decrease of the enzyme catalytic turnover from 0.31 s(-1) (0 mu M Mg2+) to 0.050 s(-1) (6000 mu M Mg2+) and a distinct shift in steady-state conformational population from one that favors the ALDH1-NADH complex with the shorter fluorescence lifetime (33% excess) in the absence of magnesium ion to one that favors the ALDH1-NADH complex with the longer fluorescence lifetime (13% excess) at 6000 mu M Mg2+. This shift in conformational population at higher Mg2+ ion concentrations and to lower enzyme activity may be due to longer residence time of the NADH in the ALDH1 pocket. The results from monitoring enzyme catalysis in the absence of magnesium suggests that the ALDH1-NADH complex with the shorter fluorescence lifetime is the form initially produced, and the complex with the longer fluorescence lifetime is produced through isomerization. Published by Elsevier Ireland Ltd. C1 [Gonnella, Thomas P.; Keating, Jennifer M.; Kjemhus, Jessica A.; Biggane, Joseph P.] Mayville State Univ, Div Sci & Math, Mayville, ND 58257 USA. [Picklo, Matthew J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. RP Gonnella, TP (reprint author), Mayville State Univ, Div Sci & Math, 330 3rd St NE, Mayville, ND 58257 USA. EM tom.gonnella@mayvillestate.edu FU NIH from the NCRR [P20 RR016471-08] FX This work was supported by the NIH grant P20 RR016471-08 (INBRE) from the NCRR. NR 17 TC 6 Z9 6 U1 1 U2 16 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PD FEB 25 PY 2013 VL 202 IS 1-3 SI SI BP 85 EP 90 DI 10.1016/j.cbi.2012.12.008 PG 6 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA 125KU UT WOS:000317539500011 PM 23295229 ER PT J AU Lu, N Sun, G Feng, XM Fu, BJ AF Lu, Nan Sun, Ge Feng, Xiaoming Fu, Bojie TI Water yield responses to climate change and variability across the North-South Transect of Eastern China (NSTEC) SO JOURNAL OF HYDROLOGY LA English DT Article DE NSTEC; Climate change; Evapotranspiration; Water yield; Spatial variability ID SOUTHEASTERN UNITED-STATES; LAND EVAPOTRANSPIRATION; STREAMFLOW RESPONSE; VEGETATION CHANGES; FOREST ECOSYSTEMS; RUNOFF; TRENDS; EVAPORATION; IMPACT; PRODUCTIVITY AB China is facing a growing water crisis due to climate and land use change, and rise in human water demand across this rapidly developing country. Understanding the spatial and temporal ecohydrologic responses to climate change is critical to sustainable water resource management. We investigated water yield (WY) responses to historical (1981-2000) and projected potential climate changes across a large and complex climatic and land cover gradients over the North-South Transect of Eastern China (NSTEC, a standard terrestrial transect of the International GeoBiological Project, IGBP). After an annual scale evapotranspiration (ET) model was validated with historical streamflow records from ten watersheds, the model was applied to the NSTEC that encompasses seven climatic zones. We found that (1) The spatial and temporal variations of WY were highly dependent on precipitation (P) patterns during 1981-2000. Overall, the influences of significant temperature (T) rise on the trend of WY were suppressed by the insignificant P change during 1981-2000. (2) The long-term mean WY by climatic zone had a similar pattern as P. The different climatic zones had differential contributions to the total volumetric WY of the NSTEC. Within each climatic zone, the volumetric WY for each land cover type was highly dependent on its area of each land cover. (3) Corresponding to the P pattern, the mean WY decreased from the low (South) to high latitude (North), but the rates of changes varied along the NSTEC. Along the NSTEC, the sensitivity of WY to potential T and P changes increased from the high latitude to the low latitude. Future potential changes in WY are likely to follow changes in P with some modification by changes in energy availability. We conclude that precipitation is a major driver for water resource availability, and reliable prediction of future precipitation change patterns is critical to hydrologic forecast across the study region. (c) 2012 Elsevier B.V. All rights reserved. C1 [Lu, Nan; Feng, Xiaoming; Fu, Bojie] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Southern Res Stn, Raleigh, NC 27606 USA. RP Lu, N (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Ecol Bldg RM301,18 Shuangqing Rd, Beijing 100085, Peoples R China. EM nanlv@rcees.ac.cn RI Fu, Bojie/B-1493-2009 FU National Basic Research Program of China [2009CB421104]; CAS/SAFEA International Partnership Program for Creative Research Teams of "Ecosystem Processes and Services"; Southern Research Station of the USDA Forest Service FX We would like to thank the two anonymous reviewers for their constructive suggestions and comments. This study is financially supported by the National Basic Research Program of China (No. 2009CB421104), and the CAS/SAFEA International Partnership Program for Creative Research Teams of "Ecosystem Processes and Services". Partial support is also from the Southern Research Station of the USDA Forest Service. NR 66 TC 12 Z9 14 U1 6 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB 25 PY 2013 VL 481 BP 96 EP 105 DI 10.1016/j.jhydrol.2012.12.020 PG 10 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 102ID UT WOS:000315837400010 ER PT J AU Martinez, G Pachepsky, YA Vereecken, H Hardelauf, H Herbst, M Vanderlinden, K AF Martinez, Gonzalo Pachepsky, Yakov A. Vereecken, Harry Hardelauf, Horst Herbst, Michael Vanderlinden, Karl TI Modeling local control effects on the temporal stability of soil water content SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil water content; Temporal stability; Simulations; Local controls; Saturated hydraulic conductivity ID SPATIAL VARIABILITY; HYDRAULIC CONDUCTIVITY; HETEROGENEOUS SOILS; SOLUTE TRANSPORT; FIELD EXPERIMENT; TIME STABILITY; MOISTURE; FLOW; PATTERNS; STORAGE AB Occurrence of temporal stability of soil water content has been observed for a range of soil and landscape conditions and is generally explained as a consequence of local and non-local controls. However, the underlying factors for this phenomenon are not completely understood and have not been quantified. This work attempts to elucidate and quantify the effects of several local controls, such as soil hydraulic properties and root water uptake, through water flow simulations. One-dimensional water flow was simulated with the HYDRUS code for bare and grassed sandy loam, loam and clay soils at different levels of variability in the saturated hydraulic conductivity K-sat. Soil water content at 0.05 and 0.60 m and the average water content of the top 1 m were analyzed. Temporal stability was characterized by calculating the mean relative differences of soil water content in 100 soil columns used for each combination of soil and season. Using log-normal distributions of K-sat resulted in mean relative differences distributions that were commonly observed in experimental studies of soil water content variability. Linear relationships were observed between scaling factor of In K-sat and spread of the mean relative differences distributions. For the same scaling factor and soil texture, simulated shapes of the mean relative differences distributions depended on the duration of the simulation period and the season. Variation in mean relative differences was higher in coarser textures than in finer ones and more variability was seen in the topsoil than in the subsoil. Root water uptake decreased the mean relative differences variability in the root zone and increased variability below it. This work presents a preliminary research to promote the use of water flow simulations under site-specific conditions to better understand the temporal stability of soil water contents. The estimation of the spatial variability of K-sat from soil water content monitoring presents an interesting avenue for further research. Published by Elsevier B.V. C1 [Martinez, Gonzalo; Pachepsky, Yakov A.] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Vereecken, Harry; Hardelauf, Horst; Herbst, Michael] Forschungszentrum Julich, Agrosphere IBG 3, Inst Bio & Geosci, D-52428 Julich, Germany. [Vanderlinden, Karl] Ctr Torres Tomejil, IFAPA, Alcala Del Rio 41200, Spain. RP Martinez, G (reprint author), 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM z42magag@uco.es RI Martinez Garcia, Gonzalo/E-3069-2013; Vanderlinden, Karl/B-5410-2008; OI Martinez Garcia, Gonzalo/0000-0001-5575-9397; Vanderlinden, Karl/0000-0002-2396-7965; Herbst, Michael/0000-0003-1371-4208; Pachepsky, Yakov/0000-0003-0232-6090 FU Spanish Ministry of Science and Innovation; FEDER [AGL2009-12936-C03-03]; Junta de Andalucia [AGR-4782]; Spanish Ministry of Education [PR2010-0191, EX2009-0433]; U.S. Nuclear Regulatory Commission; USDA FX This research was made possible thanks to funding provided by the Spanish Ministry of Science and Innovation and FEDER through Grant AGL2009-12936-C03-03 and by the Junta de Andalucia though grant AGR-4782. The mobility Grants PR2010-0191 and EX2009-0433 of the Spanish Ministry of Education are acknowledged. Partial financial support from U.S. Nuclear Regulatory Commission via the interagency agreement with USDA is appreciated. We thank reviewers for their valuable contributions and appreciate the help from the journal editor Prof. Corradini. NR 57 TC 31 Z9 31 U1 6 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB 25 PY 2013 VL 481 BP 106 EP 118 DI 10.1016/j.jhydrol.2012.12.024 PG 13 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 102ID UT WOS:000315837400011 ER PT J AU Russell, MB Woodall, CW Fraver, S D'Amato, AW AF Russell, Matthew B. Woodall, Christopher W. Fraver, Shawn D'Amato, Anthony W. TI Estimates of downed woody debris decay class transitions for forests across the eastern United States SO ECOLOGICAL MODELLING LA English DT Article DE Forest inventory; Carbon flux; Coarse woody debris; Biomass; Bioenergy ID DECOMPOSITION RATES; ACADIAN FOREST; CLIMATE-CHANGE; SITE INDEX; MANAGEMENT; DYNAMICS; MODEL; BOLES; METAANALYSIS; ATTRIBUTES AB Large-scale inventories of downed woody debris (DWD; downed dead wood of a minimum size) often record decay status by assigning pieces to classes of decay according to their visual/structural attributes (e.g., presence of branches, log shape, and texture and color of wood). DWD decay classes are not only essential for estimating current DWD biomass and carbon stocks, but may also facilitate the prediction of future DWD attributes. Estimating temporal transitions between decay classes may provide a mechanism for projecting DWD attributes in forest ecosystems. To date, modeling decay class transitions for individual DWD pieces has not been fully explored in this context. The goal of this study was to use a repeated DWD inventory across the eastern US to estimate decay class transitions to inform DWD dynamics across this broad geographic region. Using matched and non-matched DWD from the repeated inventory, ordinal regression techniques were used to estimate the five-year probability of a DWD piece remaining in the same decay class or moving into more advanced decay classes. Models indicated that these transitions were largely related to DWD piece length and climatic regime, as transitions occurred more slowly for longer DWD pieces located in regions with a low number of degree days (a climatic variable serving as a proxy for decomposition potential). Cumulative link mixed models allowed the estimation of forest type-specific effects (i.e., random effects) on the DWD transition process. Hardwood species transitioned into subsequent decay classes more rapidly than softwoods. Model assessments indicated that the correct decay class observed after five years was correctly predicted for approximately 50-70% of observations, but was dependent on forest type and initial decay class. Results differed depending on the models under examination. For example, using the matched data, the average number of classes moved per five years was 1.28 +/- 0.07 (mean +/- SE) classes for decay class 1 logs found in spruce-fir forests, however, using the matched plus non-matched data, the average number of classes moved per five years was 3.51 +/- 0.19 for these same logs. These two model sets (matched and matched plus non-matched DWD pieces) may denote upper and lower bounds for DWD decay class transition rates. Analyses presented herein provide an initial assessment of DWD decay across eastern US forests and thus provide quantitative tools that apply to emerging bioenergy questions and associated DWD dynamics research. Developed models, coupled with traditional forest productivity simulation tools, may be used in the future to determine accurate estimates of future forest C stocks. (C) 2012 Elsevier B.V. All rights reserved. C1 [Russell, Matthew B.; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Woodall, Christopher W.] USDA Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Fraver, Shawn] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Russell, MB (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM russellm@umn.edu OI Russell, Matthew/0000-0002-7044-9650; Woodall, Christopher/0000-0001-8076-6214 FU US Forest Service, Northern Research Station FX This work was supported by the US Forest Service, Northern Research Station. We thank Jeff Gove, Ron McRoberts, Joshua Puhlick, and an anonymous reviewer for their comments that improved the content of this work. NR 50 TC 8 Z9 9 U1 4 U2 57 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD FEB 24 PY 2013 VL 251 BP 22 EP 31 DI 10.1016/j.ecolmodel.2012.12.012 PG 10 WC Ecology SC Environmental Sciences & Ecology GA 121QA UT WOS:000317258100003 ER PT J AU Salazar, JK Deng, KP Tortorello, ML Brandl, MT Wang, H Zhang, W AF Salazar, Joelle K. Deng, Kaiping Tortorello, Mary Lou Brandl, Maria T. Wang, Hui Zhang, Wei TI Genes ycfR, sirA and yigG Contribute to the Surface Attachment of Salmonella enterica Typhimurium and Saintpaul to Fresh Produce SO PLOS ONE LA English DT Article ID COLI BIOFILM FORMATION; PHYLOGENETIC ANALYSIS; OXIDATIVE STRESS; RAW PRODUCE; FOOD SAFETY; PATHOGENS; RESPONSES; OUTBREAKS; PROTEIN; LIKELIHOOD AB Salmonella enterica is a frequent contaminant of minimally-processed fresh produce linked to major foodborne disease outbreaks. The molecular mechanisms underlying the association of this enteric pathogen with fresh produce remain largely unexplored. In our recent study, we showed that the expression of a putative stress regulatory gene, ycfR, was significantly induced in S. enterica upon exposure to chlorine treatment, a common industrial practice for washing and decontaminating fresh produce during minimal processing. Two additional genes, sirA involved in S. enterica biofilm formation and yigG of unknown function, were also found to be differentially regulated under chlorine stress. To further characterize the roles of ycfR, sirA, and yigG in S. enterica attachment and survival on fresh produce, we constructed in-frame deletions of all three genes in two different S. enterica serovars, Typhimurium and Saintpaul, which have been implicated in previous disease outbreaks linked to fresh produce. Bacterial attachment to glass and polystyrene microtiter plates, cell aggregation and hydrophobicity, chlorine resistance, and surface attachment to intact spinach leaf and grape tomato were compared among wild-type strains, single-gene deletion mutants, and their respective complementation mutants. The results showed that deletions of ycfR, sirA, and yigG reduced bacterial attachment to glass and polystyrene as well as fresh produce surface with or without chlorine treatment in both Typhimurium and Saintpaul. Deletion of ycfR in Typhimurium significantly reduced bacterial chlorine resistance and the attachment to the plant surfaces after chlorinated water washes. Deletions of ycfR in Typhimurium and yigG in Saintpaul resulted in significant increase in cell aggregation. Our findings suggest that ycfR, sirA, and yigG collectively contribute to S. enterica surface attachment and survival during post-harvest minimal processing of fresh produce. C1 [Salazar, Joelle K.; Zhang, Wei] IIT, Inst Food Safety & Hlth, Bedford Pk, IL USA. [Deng, Kaiping; Tortorello, Mary Lou] US FDA, Bedford Pk, IL USA. [Brandl, Maria T.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA USA. [Wang, Hui] Chinese Acad Sci, Food Safety Res Ctr, Shanghai Inst Biol Sci, Shanghai, Peoples R China. RP Zhang, W (reprint author), IIT, Inst Food Safety & Hlth, Bedford Pk, IL USA. EM zhangw@iit.edu FU United States Department of Agriculture National Institute of Food and Agriculture Grant under the Agriculture and Food Research Initiative Foundational Program Priority Area of Foodborne Pathogen-Plant Interactions [2011-67017-30016, A1301] FX This work was supported by the United States Department of Agriculture National Institute of Food and Agriculture Grant no. 2011-67017-30016 under the Agriculture and Food Research Initiative Foundational Program Priority Area of Foodborne Pathogen-Plant Interactions (Program Area Code - A1301). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 10 Z9 10 U1 3 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 22 PY 2013 VL 8 IS 2 AR e57272 DI 10.1371/journal.pone.0057272 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 113IC UT WOS:000316658800096 PM 23451197 ER PT J AU Hurkman, WJ Tanaka, CK Vensel, WH Thilmony, R Altenbach, SB AF Hurkman, William J. Tanaka, Charlene K. Vensel, William H. Thilmony, Roger Altenbach, Susan B. TI Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86 SO PROTEOME SCIENCE LA English DT Article DE Endosperm; Fertilizer; Flour; Gliadins; Glutenins; Proteome; Temperature; Wheat ID WHEAT-GRAIN DEVELOPMENT; PROTEIN-COMPOSITION; POSTANTHESIS FERTILIZER; POLYMERIC PROTEIN; BIOCHEMICAL BASIS; BREAD WHEATS; WINTER-WHEAT; HEAT-STRESS; GENE FAMILY; NITROGEN AB Background: Flour quality is largely determined by the gluten proteins, a complex mixture of proteins consisting of high molecular weight-glutenin subunits (HMW-GS), low molecular weight-glutenin subunits (LMW-GS), and alpha-, gamma-, and omega-gliadins. Detailed proteomic analyses of the effects of fertilizer and high temperature on individual gliadin and glutenin protein levels are needed to determine how these environmental factors influence flour quality. Results: Wheat plants (Triticum aestivum L. cv. Butte 86) were grown in greenhouses under moderate and high temperature regimens with and without post-anthesis fertilizer. Quantitative two-dimensional gel electrophoresis was used to construct accumulation profiles in developing endosperm for the entire complement of gluten proteins identified previously by tandem mass spectrometry. Amounts of individual gliadins and glutenins were also determined in flour produced under each of the regimens. Under all environmental regimens, most HMW-GS, LMW-GS, gamma- and omega-gliadins accumulated rapidly during early stages of grain development and leveled off during middle stages of development. A subset of LMW-GS showed a second distinct profile, accumulating throughout development, while alpha-gliadins showed a variety of accumulation profiles. In flour, fourteen distinct gluten proteins responded similarly to fertilizer, high temperature, and high temperature plus fertilizer. The majority of HMW-GS and omega-gliadins and some alpha-gliadins increased while two LMW-GS and a minor gamma-gliadin decreased. Fertilizer did not influence gluten protein accumulation under high temperature conditions. Additionally, the effects of fertilizer and high temperature were not additive; very few changes were observed when plants that received fertilizer were subjected to high temperature. Conclusions: Although post-anthesis temperature and fertilizer have very different effects on grain development and yield, the two treatments elicit surprisingly similar effects on the accumulation of gluten proteins. The similarity of the responses to the different treatments is likely due to source-sink activities of nitrogen reserves in the wheat plant. Because each protein that showed a response in this study is linked to a gene sequence, the work sets the stage for transgenic studies that will better elucidate the roles of specific proteins in flour quality and in the response to the environment. C1 [Hurkman, William J.; Tanaka, Charlene K.; Vensel, William H.; Thilmony, Roger; Altenbach, Susan B.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Altenbach, SB (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM susan.altenbach@ars.usda.gov OI Vensel, William/0000-0001-9454-2705 FU USDA Agricultural Service CRIS [5325-43000-027-00D] FX This research was funded by USDA Agricultural Service CRIS Project 5325-43000-027-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation over or endorsement by the U. S. Department of Agriculture. The U. S. Department of Agriculture is an equal opportunity provider and employer. NR 50 TC 18 Z9 18 U1 4 U2 53 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1477-5956 J9 PROTEOME SCI JI Proteome Sci. PD FEB 22 PY 2013 VL 11 AR 8 DI 10.1186/1477-5956-11-8 PG 15 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 119UT UT WOS:000317124900001 PM 23432757 ER PT J AU Spackman, E Pedersen, JC McKinley, ET Gelb, J AF Spackman, Erica Pedersen, Janice C. McKinley, Enid T. Gelb, Jack, Jr. TI Optimal specimen collection and transport methods for the detection of avian influenza virus and Newcastle disease virus SO BMC VETERINARY RESEARCH LA English DT Article ID REVERSE-TRANSCRIPTION-PCR; POOLING CERVICAL SWABS; CHLAMYDIA-TRACHOMATIS; CHAIN-REACTION; FLOCKED SWABS; DIAGNOSIS; SAMPLES; ASSAY AB Background: Active and passive surveillance for avian influenza virus (AIV) and Newcastle disease virus (NDV) is widespread in commercial poultry worldwide, therefore optimization of sample collection and transport would be valuable to achieve the best sensitivity and specificity possible, and to develop the most accurate and efficient testing programs. A H7N2 low pathogenicity (LP) AIV strain was selected and used as an indicator virus because it is present in lower concentrations in swabbings and thus requires greater sensitivity for detection compared to highly pathogenic (HP) AIV. For similar reasons a mesogenic strain of NDV was selected. Using oro-pharyngeal and cloacal swabs collected from chickens experimentally exposed to the viruses we evaluated the effects of numerous aspects of sample collection and transport: 1) swab construction material (flocked nylon, non-flocked Dacron, or urethane foam), 2) transport media (brain heart infusion broth [BHI] or phosphate buffered saline [PBS]), 3) media volume (2 ml or 3.5 ml), 4) transporting the swab wet in the vial or removing the swab prior to transport, or transporting the swab dry with no media, and 5) single swabs versus pooling 5 or 11 swabs per vial. Results: Using real-time RT-PCR (rRT-PCR), virus isolation (VI) and commercial antigen detection immunoassays for AIV we observed statistically significant differences and consistent trends with some elements of sample collection and transport; media, dry transport and swab construction. Conversely, the number of swabs pooled (1, 5 or 11) and whether the swab was removed prior to transport did not impact virus detection. Similarly, with NDV detection by both VI and rRT-PCR was not affected by the numbers of swabs collected in a single vial (1, 5 or 11). Conclusions: We observed that flocked and foam swabs were superior to non-flocked swabs, BHI media was better than PBS, and transporting swabs wet was better for virus recovery and detection than transporting them dry. There was no observable difference in detection whether the swab was removed prior to transport or left in the vial. Also, with both AIV and NDV, there was no observed difference in virus detection between pools of 1, 5 or 11 swabs. C1 [Spackman, Erica; McKinley, Enid T.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. [Pedersen, Janice C.] USDA APHIS, Natl Vet Serv Labs, Ames, IA USA. [Gelb, Jack, Jr.] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. RP Spackman, E (reprint author), ARS, Southeast Poultry Res Lab, USDA, 934 Coll,Stn Rd, Athens, GA 30605 USA. EM Erica.spackman@ars.usda.gov FU US Poultry and Egg Association project [663]; USDA-ARS CRIS Project [6612-32000-063-00D] FX Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. The authors would like to thank Scott Lee, Mary Lea Killian, Jack King, Joan Beck, Diane Smith, Kira Moresco, Ron Graham, Roger Brock, and Anna Linz for technical assistance with this work. This work was supported by US Poultry and Egg Association project #663 and USDA-ARS CRIS Project #6612-32000-063-00D. NR 18 TC 7 Z9 7 U1 0 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD FEB 22 PY 2013 VL 9 AR 35 DI 10.1186/1746-6148-9-35 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA 108ET UT WOS:000316275300001 PM 23432911 ER PT J AU Song, LY Lu, WX Hu, J Yin, WB Chen, YH Wang, BL Wang, RRC Hu, ZM AF Song, Li-Ying Lu, Wan-Xiang Hu, Jun Yin, Wei-Bo Chen, Yu-Hong Wang, Bai-Lin Wang, Richard R. -C. Hu, Zan-Min TI The role of C-terminal amino acid residues of a Delta(6)-fatty acid desaturase from blackcurrant SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article DE Delta(6)-Fatty acid desaturase; Delta(8)-Sphingolipid desaturase; C-terminus; Amino acid residue; Enzyme catalysis activity ID REGIOSELECTIVITY; MUTAGENESIS; DOMAIN; DELTA(6)-DESATURASE; EVOLUTION; PROTEINS AB Delta(6)-fatty acid desaturase is an important enzyme in the catalytic synthesis of polyunsaturated fatty acids. Using domain swapping and a site-directed mutagenesis strategy, we found that the region of the C-terminal 67 amino acid residues of Delta(6)-fatty acid desaturase RnD6C from blackcurrant was essential for its catalytic activity and that seven different residues between RnD6C and RnD8A in that region were involved in the desaturase activity. Compared with RnD6C, the activity of the following mutations, V394A, IC395I, F411L, S436P, VIC3945AI and IS4356VP, was significantly decreased, whereas the activity of 1417T was significantly increased. The amino acids N, T and Y in the last four residues also play a certain role in the desaturase activity. (C) 2013 Elsevier Inc. All rights reserved. C1 [Song, Li-Ying; Lu, Wan-Xiang; Hu, Jun; Yin, Wei-Bo; Chen, Yu-Hong; Hu, Zan-Min] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China. [Lu, Wan-Xiang] Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400716, Peoples R China. [Wang, Bai-Lin] Heilongjiang Agr Acad, Hort Div, Harbin 150069, Peoples R China. [Wang, Richard R. -C.] Utah State Univ, USDA ARS, FRRL, Logan, UT 84322 USA. RP Hu, ZM (reprint author), Chinese Acad Sci, Inst Genet & Dev Biol, Datun Rd, Beijing 100101, Peoples R China. EM lysong@genetics.ac.cn; wanxianglu@126.com; junhu7626@yahoo.com; wbyin@genetics.ac.cn; yhchen@genetics.ac.cn; wangbailin1@l26.com; Richard.Wang@ARS.USDA.GOV; zmhu@genetics.ac.cn FU Ministry of Agriculture of China for transgenic research [2011ZX08009-003-004]; Ministry of Science and Technology of China [2011CB200902] FX This research was supported by a project (2011ZX08009-003-004) from the Ministry of Agriculture of China for transgenic research and a project (2011CB200902) from the Ministry of Science and Technology of China. NR 16 TC 5 Z9 5 U1 0 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 22 PY 2013 VL 431 IS 4 BP 675 EP 679 DI 10.1016/j.bbrc.2013.01.062 PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 103QZ UT WOS:000315935300006 PM 23357423 ER PT J AU Heizer, E Zarlenga, DS Rosa, B Gao, X Gasser, RB De Graef, J Geldhof, P Mitreva, M AF Heizer, Esley Zarlenga, Dante S. Rosa, Bruce Gao, Xin Gasser, Robin B. De Graef, Jessie Geldhof, Peter Mitreva, Makedonka TI Transcriptome analyses reveal protein and domain families that delineate stage-related development in the economically important parasitic nematodes, Ostertagia ostertagi and Cooperia oncophora SO BMC GENOMICS LA English DT Article DE Cattle; Parasite; Nematode; Transcripts; Ostertagia ostertagi; Cooperia oncophora; Comparative genomics ID CAENORHABDITIS-ELEGANS; ANCYLOSTOMA-CANINUM; NECATOR-AMERICANUS; DRUG-RESISTANCE; PHYLUM NEMATODA; GENE-EXPRESSION; GENOMES; HOOKWORM; CATTLE; DATABASE AB Background: Cooperia oncophora and Ostertagia ostertagi are among the most important gastrointestinal nematodes of cattle worldwide. The economic losses caused by these parasites are on the order of hundreds of millions of dollars per year. Conventional treatment of these parasites is through anthelmintic drugs; however, as resistance to anthelmintics increases, overall effectiveness has begun decreasing. New methods of control and alternative drug targets are necessary. In-depth analysis of transcriptomic data can help provide these targets. Results: The assembly of 8.7 million and 11 million sequences from C. oncophora and O. ostertagi, respectively, resulted in 29,900 and 34,792 transcripts. Among these, 69% and 73% of the predicted peptides encoded by C. oncophora and O. ostertagi had homologues in other nematodes. Approximately 21% and 24% were constitutively expressed in both species, respectively; however, the numbers of transcripts that were stage specific were much smaller (similar to 1% of the transcripts expressed in a stage). Approximately 21% of the transcripts in C. oncophora and 22% in O. ostertagi were up-regulated in a particular stage. Functional molecular signatures were detected for 46% and 35% of the transcripts in C. oncophora and O. ostertagi, respectively. More in-depth examinations of the most prevalent domains led to knowledge of gene expression changes between the free-living (egg, L1, L2 and L3 sheathed) and parasitic (L3 exsheathed, L4, and adult) stages. Domains previously implicated in growth and development such as chromo domains and the MADF domain tended to dominate in the free-living stages. In contrast, domains potentially involved in feeding such as the zinc finger and CAP domains dominated in the parasitic stages. Pathway analyses showed significant associations between life-cycle stages and peptides involved in energy metabolism in O. ostertagi whereas metabolism of cofactors and vitamins were specifically up-regulated in the parasitic stages of C. oncophora. Substantial differences were observed also between Gene Ontology terms associated with free-living and parasitic stages. Conclusions: This study characterized transcriptomes from multiple life stages from both C. oncophora and O. ostertagi. These data represent an important resource for studying these parasites. The results of this study show distinct differences in the genes involved in the free-living and parasitic life cycle stages. The data produced will enable better annotation of the upcoming genome sequences and will allow future comparative analyses of the biology, evolution and adaptation to parasitism in nematodes. C1 [Heizer, Esley; Rosa, Bruce; Gao, Xin; Mitreva, Makedonka] Washington Univ, Sch Med, Genome Inst, St Louis, MO 63108 USA. [Zarlenga, Dante S.] Agr Res Serv, USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Gasser, Robin B.] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia. [De Graef, Jessie; Geldhof, Peter] Univ Ghent, Fac Vet Med, Dept Virol Parasitol & Immunol, B-9820 Merelbeke, Belgium. [Mitreva, Makedonka] Washington Univ, Sch Med, Dept Med, Div Infect Dis, St Louis, MO 63110 USA. [Mitreva, Makedonka] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63108 USA. RP Mitreva, M (reprint author), Washington Univ, Sch Med, Genome Inst, St Louis, MO 63108 USA. EM mmitreva@genome.wustl.edu RI Gao, Cyan/O-9316-2014 FU National Institute Of Allergy And Infectious Diseases of the National Institutes of Health [R01AI081803]; NHGRI [U54HG003079]; 'Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT - Vlaanderen) FX We would like to thank the dedicated members of the cDNA production group at the Washington University's Genome Institute, John Martin, Zhengyuan Wang and Qi Wang for technical assistance and to all authors of the numerous algorithms used to perform the analysis. Research reported in this publication and the Nematode.net data dissemination was supported by the National Institute Of Allergy And Infectious Diseases of the National Institutes of Health under Award Number R01AI081803 to M. M. The cDNA pyrosequencing is part of the Strongylida genome sequencing initiative at the WUGC supported by NHGRI (U54HG003079)(http://www.genome.gov/10002154). JDG was funded by a Ph.D. grant of the 'Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT - Vlaanderen). NR 50 TC 4 Z9 4 U1 0 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 22 PY 2013 VL 14 AR 118 DI 10.1186/1471-2164-14-118 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 104QA UT WOS:000316008200001 PM 23432754 ER PT J AU Walters, C Berjak, P Pammenter, N Kennedy, K Raven, P AF Walters, Christina Berjak, Patricia Pammenter, Norman Kennedy, Kathryn Raven, Peter TI Preservation of Recalcitrant Seeds SO SCIENCE LA English DT Editorial Material ID DESICCATION SENSITIVITY; CRYOPRESERVATION C1 [Walters, Christina] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Berjak, Patricia; Pammenter, Norman] Univ KwaZulu Natal, Sch Life Sci, ZA-4001 Durban, South Africa. [Kennedy, Kathryn] Ctr Plant Conservat, St Louis, MO 63166 USA. [Raven, Peter] Missouri Bot Garden, St Louis, MO 63166 USA. RP Walters, C (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. EM christina.walters@ars.usda.gov NR 20 TC 29 Z9 34 U1 4 U2 77 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD FEB 22 PY 2013 VL 339 IS 6122 BP 915 EP 916 DI 10.1126/science.1230935 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 092UX UT WOS:000315149600036 PM 23430644 ER PT J AU Dassanayake, RP Shanthalingam, S Subramaniam, R Herndon, CN Bavananthasivam, J Haldorson, GJ Foreyt, WJ Evermann, JF Herrmann-Hoesing, LM Knowles, DP Srikumaran, S AF Dassanayake, Rohana P. Shanthalingam, Sudarvili Subramaniam, Renuka Herndon, Caroline N. Bavananthasivam, Jegarubee Haldorson, Gary J. Foreyt, William J. Evermann, James F. Herrmann-Hoesing, Lynn M. Knowles, Donald P. Srikumaran, Subramaniam TI Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia SO VETERINARY MICROBIOLOGY LA English DT Article DE Bighorn sheep; Pneumonia; Bibersteinia trehalosi; Mannheimia haemolytica; Respiratory syncytial virus; Parainfluenza-3 virus ID OVIS-CANADENSIS-CANADENSIS; PASTEURELLA-HAEMOLYTICA; MANNHEIMIA-HAEMOLYTICA; MYCOPLASMA-OVIPNEUMONIAE; DOMESTIC SHEEP; BOVINE HERPESVIRUS-1; DISEASE; INFECTION AB Pneumonic bighorn sheep (BHS) have been found to be culture- and/or sero-positive for Bibersteinia trehalosi, respiratory syncytial virus (RSV), and parainfluenza-3 virus (PI-3). The objective of this study was to determine whether these pathogens can cause fatal pneumonia in BHS. In the first study, two groups of four BHS each were intra-tracheally administered with leukotoxin-positive (Group I) or leukotoxin-negative (Group II) B. trehalosi. All four animals in Group I developed severe pneumonia, and two of them died within 3 days. The other two animals showed severe pneumonic lesions on euthanasia and necropsy. Animals in Group II neither died nor showed gross pneumonic lesions on necropsy, suggesting that leukotoxin-positive, but not leukotoxin-negative, B. trehalosi can cause fatal pneumonia in BHS. In the second study, two other groups of four BHS (Groups III and IV) were intra-nasally administered with a mixture of RSV and PI-3. Four days later, RSV/PI-3-inoculated Group IV and another group of four BHS (Group V, positive control) were intra-nasally administered with Mannheimia haemolytica, the pathogen that consistently causes fatal pneumonia in BHS. All four animals in group III developed pneumonia, but did not die during the study period. However all four animals in Group IV, and three animals in Group V developed severe pneumonia and died within two days of M. haemolytica inoculation. The fourth animal in Group V showed severe pneumonic lesions on euthanasia and necropsy. These findings suggest that RSV/PI-3 can cause non-fatal pneumonia, but are not necessary predisposing agents for M. haemolytica-caused pneumonia of BHS. (C) 2012 Elsevier B.V. All rights reserved. C1 [Dassanayake, Rohana P.; Shanthalingam, Sudarvili; Subramaniam, Renuka; Herndon, Caroline N.; Bavananthasivam, Jegarubee; Haldorson, Gary J.; Foreyt, William J.; Evermann, James F.; Knowles, Donald P.; Srikumaran, Subramaniam] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Haldorson, Gary J.; Evermann, James F.] Washington State Univ, Washington Anim Dis Diagnost Lab, Pullman, WA 99164 USA. [Herrmann-Hoesing, Lynn M.; Knowles, Donald P.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. RP Srikumaran, S (reprint author), Washington State Univ, Dept Pathol & Microbiol, Pullman, WA 99164 USA. EM ssrikumaran@vetmed.wsu.edu FU Foundation for North American Wild Sheep FX This research was supported by funds from the Foundation for North American Wild Sheep and its Eastern, Idaho, Oregon, and Washington Chapters. We thank Dr. Howard Lehmkuhl, National Animal Disease Center, Ames, Iowa for providing us ovine strain of PI-3 virus (DH-1) and ovine fetal tracheal cells. We thank Mr. John Vanderchalie, Ms. Lorraine Tanaka, and Ms. Sara Schlee of WADDL for assistance with the serum neutralization assay. We also thank Ms. Jennifer Baisley of WADDL for assistance processing samples for virus isolation. NR 24 TC 7 Z9 7 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 J9 VET MICROBIOL JI Vet. Microbiol. PD FEB 22 PY 2013 VL 162 IS 1 BP 166 EP 172 DI 10.1016/j.vetmic.2012.08.029 PG 7 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 090TL UT WOS:000315002800020 PM 23025980 ER PT J AU Haynes, KJ Bjornstad, ON Allstadt, AJ Liebhold, AM AF Haynes, Kyle J. Bjornstad, Ottar N. Allstadt, Andrew J. Liebhold, Andrew M. TI Geographical variation in the spatial synchrony of a forest-defoliating insect: isolation of environmental and spatial drivers SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE dispersal; insect outbreaks; Moran effect; oak masting ID GYPSY-MOTH OUTBREAKS; POPULATION-DYNAMICS; NORTH-AMERICA; ENTOMOPHAGA-MAIMAIGA; MULTIPLE-REGRESSION; ACORN MAST; PATTERNS; DISTANCE; FLUCTUATIONS; LEPIDOPTERA AB Despite the pervasiveness of spatial synchrony of population fluctuations in virtually every taxon, it remains difficult to disentangle its underlying mechanisms, such as environmental perturbations and dispersal. We used multiple regression of distance matrices (MRMs) to statistically partition the importance of several factors potentially synchronizing the dynamics of the gypsy moth, an invasive species in North America, exhibiting outbreaks that are partially synchronized over long distances (approx. 900 km). The factors considered in the MRM were synchrony in weather conditions, spatial proximity and forest-type similarity. We found that the most likely driver of outbreak synchrony is synchronous precipitation. Proximity played no apparent role in influencing outbreak synchrony after accounting for precipitation, suggesting dispersal does not drive outbreak synchrony. Because a previous modelling study indicated weather might indirectly synchronize outbreaks through synchronization of oak masting and generalist predators that feed upon acorns, we also examined the influence of weather and proximity on synchrony of acorn production. As we found for outbreak synchrony, synchrony in oak masting increased with synchrony in precipitation, though it also increased with proximity. We conclude that precipitation could synchronize gypsy moth populations directly, as in a Moran effect, or indirectly, through effects on oak masting, generalist predators or diseases. C1 [Haynes, Kyle J.; Allstadt, Andrew J.] Univ Virginia, Boyce, VA 22620 USA. [Bjornstad, Ottar N.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Liebhold, Andrew M.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. RP Haynes, KJ (reprint author), Univ Virginia, 400 Blandy Farm Lane, Boyce, VA 22620 USA. EM haynes@virginia.edu RI Haynes, Kyle/C-1374-2012; Liebhold, Andrew/C-1423-2008; OI Haynes, Kyle/0000-0002-3283-6633; Liebhold, Andrew/0000-0001-7427-6534; Allstadt, Andrew/0000-0003-3915-0834 FU National Science Foundation; US Department of Homeland Security; US Department of Agriculture through NSF [EF-0832858]; University of Tennessee, Knoxville; USDA AFRI grant; National Science Foundation [DEB 1020614] FX We thank G. Luzader for assistance in preparing data, and G. Norman, T. Fearer, the Virginia Department of Game and Inland Fisheries, and the Maryland Department of Natural Resources for access to oak masting data. This manuscript benefitted from comments provided by T. Roulston, R. Malfi and J. Walter. This work was partially conducted as a part of the 'Forest Insect Population Dynamics' Working Group at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation, the US Department of Homeland Security and the US Department of Agriculture through NSF Award no. EF-0832858, with additional support from the University of Tennessee, Knoxville, USDA AFRI grant to O.N.B., and a National Science Foundation grant to K.J.H. (DEB 1020614). NR 50 TC 11 Z9 11 U1 4 U2 89 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD FEB 22 PY 2013 VL 280 IS 1753 AR 20122373 DI 10.1098/rspb.2012.2373 PG 8 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA 070ML UT WOS:000313510600005 PM 23282993 ER PT J AU Badke, YM Bates, RO Ernst, CW Schwab, C Fix, J Van Tassell, CP Steibel, JP AF Badke, Yvonne M. Bates, Ronald O. Ernst, Catherine W. Schwab, Clint Fix, Justin Van Tassell, Curtis P. Steibel, Juan P. TI N Methods of tagSNP selection and other variables affecting imputation accuracy in swine SO BMC GENETICS LA English DT Article DE Genotype imputation; Pigs; Reference panel size ID DENSITY MARKER PANELS; NUCLEOTIDE POLYMORPHISM GENOTYPES; INFORMATIVE SNP SELECTION; LINKAGE DISEQUILIBRIUM; UNRELATED INDIVIDUALS; GENOMIC SELECTION; JERSEY CATTLE; DAIRY-CATTLE; POPULATIONS; PREDICTION AB Background: Genotype imputation is a cost efficient alternative to use of high density genotypes for implementing genomic selection. The objective of this study was to investigate variables affecting imputation accuracy from low density tagSNP (average distance between tagSNP from 100kb to 1Mb) sets in swine, selected using LD information, physical location, or accuracy for genotype imputation. We compared results of imputation accuracy based on several sets of low density tagSNP of varying densities and selected using three different methods. In addition, we assessed the effect of varying size and composition of the reference panel of haplotypes used for imputation. Results: TagSNP density of at least 1 tagSNP per 340kb (similar to 7000 tagSNP) selected using pairwise LD information was necessary to achieve average imputation accuracy higher than 0.95. A commercial low density (9K) tagSNP set for swine was developed concurrent to this study and an average accuracy of imputation of 0.951 based on these tagSNP was estimated. Construction of a haplotype reference panel was most efficient when these haplotypes were obtained from randomly sampled individuals. Increasing the size of the original reference haplotype panel (128 haplotypes sampled from 32 sire/dam/offspring trios phased in a previous study) led to an overall increase in imputation accuracy (IA = 0.97 with 512 haplotypes), but was especially useful in increasing imputation accuracy of SNP with MAF below 0.1 and for SNP located in the chromosomal extremes (within 5% of chromosome end). Conclusion: The new commercially available 9K tagSNP set can be used to obtain imputed genotypes with high accuracy, even when imputation is based on a comparably small panel of reference haplotypes (128 haplotypes). Average imputation accuracy can be further increased by adding haplotypes to the reference panel. In addition, our results show that randomly sampling individuals to genotype for the construction of a reference haplotype panel is more cost efficient than specifically sampling older animals or trios with no observed loss in imputation accuracy. We expect that the use of imputed genotypes in swine breeding will yield highly accurate predictions of GEBV, based on the observed accuracy and reported results in dairy cattle, where genomic evaluation of some individuals is based on genotypes imputed with the same accuracy as our Yorkshire population. C1 [Badke, Yvonne M.; Bates, Ronald O.; Ernst, Catherine W.; Steibel, Juan P.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Schwab, Clint] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA. [Fix, Justin] Maschhoffs, Carlyle, IL USA. [Van Tassell, Curtis P.] USDA ARS, Bovine Funct Genom Lab, Beltsville, MD USA. RP Steibel, JP (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2010-65205-20342]; National Swine Registry FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010-65205-20342 from the USDA National Institute of Food and Agriculture, and by funding from the National Swine Registry. Technical assistance of Nancy Raney and Whitney Hosier is appreciated. Computer resources and programming advice were provided by the Michigan State University High Performance Computing Center (HPCC). NR 40 TC 19 Z9 19 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD FEB 21 PY 2013 VL 14 AR UNSP 8 DI 10.1186/1471-2156-14-8 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA 196SR UT WOS:000322797100001 PM 23433396 ER PT J AU Wu, X Vellaichamy, A Wang, DP Zamdborg, L Kelleher, NL Huber, SC Zhao, YF AF Wu, Xia Vellaichamy, Adaikkalam Wang, Dongping Zamdborg, Leonid Kelleher, Neil L. Huber, Steven C. Zhao, Youfu TI Differential lysine acetylation profiles of Erwinia amylovora strains revealed by proteomics SO JOURNAL OF PROTEOMICS LA English DT Article DE Acetylome; Metabolism; Fire blight; Virulence; Type III secretion system; Exopolysaccharide ID FIRE BLIGHT PATHOGEN; PROTEIN ACETYLATION; ESCHERICHIA-COLI; ISOCITRATE DEHYDROGENASE; VIRULENCE DETERMINANT; SUBSTRATE-SPECIFICITY; RESPONSE REGULATOR; CRYSTAL-STRUCTURE; DIVERSE FUNCTION; COA SYNTHETASE AB Protein lysine acetylation (LysAc) has recently been demonstrated to be widespread in E. coli and Salmonella, and to broadly regulate bacterial physiology and metabolism. However, LysAc in plant pathogenic bacteria is largely unknown. Here we first report the lysine acetylome of Erwinia amylovora, an enterobacterium causing serious fire blight disease of apples and pears. Immunoblots using generic anti-lysine acetylation antibodies demonstrated that growth conditions strongly affected the LysAc profiles in E. amylovora. Differential LysAc profiles were also observed for two E. amylovora strains, known to have differential virulence in plants, indicating translational modification of proteins may be important in determining virulence of bacterial strains. Proteomic analysis of LysAc in two E. amylovora strains identified 141 LysAc sites in 96 proteins that function in a wide range of biological pathways. Consistent with previous reports, 44% of the proteins are involved in metabolic processes, including central metabolism, lipopolysaccharide, nucleotide and amino acid metabolism. Interestingly, for the first time, several proteins involved in E. amylovora virulence, including exopolysaccharide amylovoran biosynthesis- and type III secretion-associated proteins, were found to be lysine acetylated, suggesting that LysAc may play a major role in bacterial virulence. Comparative analysis of LysAc sites in E. amylovora and E. coli further revealed the sequence and structural commonality for LysAc in the two organisms. Collectively, these results reinforce the notion that LysAc of proteins is widespread in bacterial metabolism and virulence. (C) 2012 Elsevier B.V. All rights reserved. C1 [Wu, Xia; Huber, Steven C.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Vellaichamy, Adaikkalam; Zamdborg, Leonid; Kelleher, Neil L.] Univ Illinois, Dept Chem, Urbana, IL 61801 USA. [Vellaichamy, Adaikkalam; Zamdborg, Leonid; Kelleher, Neil L.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Wang, Dongping; Zhao, Youfu] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Kelleher, Neil L.] Northwestern Univ, Dept Chem & Mol Biosci, Evanston, IL 60208 USA. [Kelleher, Neil L.] Northwestern Univ, Feinberg Sch Med, Evanston, IL 60208 USA. [Huber, Steven C.] USDA ARS, Urbana, IL 61801 USA. RP Huber, SC (reprint author), 1201 W Gregory Dr,197 ERML, Urbana, IL 61801 USA. EM schuber1@illinois.edu; zhao888@illinois.edu RI Wang, Dongping /L-2124-2013; Wu, Xia/J-8690-2014 OI Wu, Xia/0000-0002-0024-4481 FU Campus Research Board, University of Illinois [10192]; Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2010-65110-20497]; NIDA [1F30DA026672] FX We thank Drs. Furong Sun and Haijun Yao for the help on MALDI-TOF analysis and Dr. P. Yau at the proteomics facility, Keck Center of the University of Illinois for helpful discussions. We also thank the Campus Research Board, University of Illinois for providing partial funding for this research (Award #10192). This project was supported by the Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2010-65110-20497 from the USDA National Institute of Food and Agriculture (YFZ). LZ is supported by NIDA grant 1F30DA026672. NR 67 TC 39 Z9 41 U1 3 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 J9 J PROTEOMICS JI J. Proteomics PD FEB 21 PY 2013 VL 79 BP 60 EP 71 DI 10.1016/j.jprot.2012.12.001 PG 12 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 154AE UT WOS:000319643100005 PM 23234799 ER PT J AU Campos, GEP Moran, MS Huete, A Zhang, YG Bresloff, C Huxman, TE Eamus, D Bosch, DD Buda, AR Gunter, SA Scalley, TH Kitchen, SG McClaran, MP McNab, WH Montoya, DS Morgan, JA Peters, DPC Sadler, EJ Seyfried, MS Starks, PJ AF Campos, Guillermo E. Ponce Moran, M. Susan Huete, Alfredo Zhang, Yongguang Bresloff, Cynthia Huxman, Travis E. Eamus, Derek Bosch, David D. Buda, Anthony R. Gunter, Stacey A. Scalley, Tamara Heartsill Kitchen, Stanley G. McClaran, Mitchel P. McNab, W. Henry Montoya, Diane S. Morgan, Jack A. Peters, Debra P. C. Sadler, E. John Seyfried, Mark S. Starks, Patrick J. TI Ecosystem resilience despite large-scale altered hydroclimatic conditions SO NATURE LA English DT Article ID RAIN-USE EFFICIENCY; CLIMATE-CHANGE; DROUGHT; WATER; EVAPOTRANSPIRATION; BIOMES AB Climate change is predicted to increase both drought frequency and duration, and when coupled with substantial warming, will establish a new hydroclimatological model for many regions(1). Large-scale, warm droughts have recently occurred in North America, Africa, Europe, Amazonia and Australia, resulting in major effects on terrestrial ecosystems, carbon balance and food security(2,3). Here we compare the functional response of above-ground net primary production to contrasting hydroclimatic periods in the late twentieth century (1975-1998), and drier, warmer conditions in the early twenty-first century (2000-2009) in the Northern and Southern Hemispheres. We find a common ecosystem water-use efficiency (WUEe: above-ground net primary production/evapotranspiration) across biomes ranging from grassland to forest that indicates an intrinsic system sensitivity to water availability across rainfall regimes, regardless of hydroclimatic conditions. We found higher WUEe in drier years that increased significantly with drought to a maximum WUEe across all biomes; and a minimum native state in wetter years that was common across hydroclimatic periods. This indicates biome-scale resilience to the interannual variability associated with the early twenty-first century drought-that is, the capacity to tolerate low, annual precipitation and to respond to subsequent periods of favourable water balance. These findings provide a conceptual model of ecosystem properties at the decadal scale applicable to the widespread altered hydroclimatic conditions that are predicted for later this century. Understanding the hydroclimatic threshold that will break down ecosystem resilience and alter maximum WUEe may allow us to predict land-surface consequences as large regions become more arid, starting with water-limited, low-productivity grasslands. C1 [Campos, Guillermo E. Ponce; Moran, M. Susan; Zhang, Yongguang] ARS SW Watershed Res, USDA, Tucson, AZ 85719 USA. [Campos, Guillermo E. Ponce; Bresloff, Cynthia] Univ Arizona, Tucson, AZ 85721 USA. [Huete, Alfredo; Eamus, Derek] Univ Technol Sydney, Sydney, NSW 2007, Australia. [Huxman, Travis E.] Univ Calif Irvine, Irvine, CA USA. [Huxman, Travis E.] Univ Calif Irvine, Ctr Environm Biol, Irvine, CA 92697 USA. [Bosch, David D.] ARS, USDA, SE Watershed Res Lab, Tifton, GA 31793 USA. [Buda, Anthony R.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Gunter, Stacey A.] ARS, USDA, So Plains Range Res Stn, Woodward, OK 73801 USA. [Scalley, Tamara Heartsill] USDA FS Int Inst Trop Forestry, Rio Piedras, PR 00926 USA. [Kitchen, Stanley G.] USDA, FS Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. [McClaran, Mitchel P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [McNab, W. Henry] USDA FS So Res Stn, Asheville, NC 28806 USA. [Montoya, Diane S.] USDA FS Pacific SW Res Stn, Arcata, CA 95521 USA. [Morgan, Jack A.] ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Peters, Debra P. C.] New Mexico State Univ, USDA ARS Jornada Expt Range & Jornada Basin Long, Las Cruces, NM 88012 USA. [Sadler, E. John] ARS, USDA, Cropping Syst & Water Qual Res Unit, Columbia, MO 65211 USA. [Seyfried, Mark S.] ARS, USDA, NW Watershed Res Ctr, Boise, ID 83712 USA. [Starks, Patrick J.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Campos, GEP (reprint author), ARS SW Watershed Res, USDA, Tucson, AZ 85719 USA. EM geponce@gmail.com; susan.moran@ars.usda.gov RI Huete, Alfredo/C-1294-2008; OI Huete, Alfredo/0000-0003-2809-2376; Heartsill Scalley, Tamara/0000-0003-0550-4147; Eamus, Derek/0000-0003-2765-8040; Zhang, Yongguang/0000-0001-8286-300X FU NASA SMAP Science Definition Team [08-SMAPSDT08-0042]; Australian Research Council (ARC) [DP1115479]; Terrestrial Ecosystem Research Network (TERN) EIF: AusCover FX The work was-supported in part by the NASA SMAP Science Definition Team under agreement 08-SMAPSDT08-0042, the Australian Research Council (ARC) Discover Project (DP1115479) and the Terrestrial Ecosystem Research Network (TERN) EIF: AusCover. We thank the Australian Bureau of Meteorology for providing the precipitation data. We also thank J. Overpeck, T. McVicar, R. Donohue and M. Walbridge for their input. NR 22 TC 80 Z9 81 U1 20 U2 315 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD FEB 21 PY 2013 VL 494 IS 7437 BP 349 EP 352 DI 10.1038/nature11836 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 095CS UT WOS:000315312900037 ER PT J AU Nagy-Szakal, D Hollister, EB Luna, RA Szigeti, R Tatevian, N Smith, CW Versalovic, J Kellermayer, R AF Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Szigeti, Reka Tatevian, Nina Smith, C. Wayne Versalovic, James Kellermayer, Richard TI Cellulose Supplementation Early in Life Ameliorates Colitis in Adult Mice SO PLOS ONE LA English DT Article ID INFLAMMATORY BOWEL DISEASES; DEXTRAN SULFATE SODIUM; HUMAN LARGE-INTESTINE; DIETARY FIBER; ULCERATIVE-COLITIS; COLONIC-MUCOSA; GUT MICROBIOTA; FERMENTATION; DIVERSITY; SEQUENCES AB Decreased consumption of dietary fibers, such as cellulose, has been proposed to promote the emergence of inflammatory bowel diseases (IBD: Crohn disease [CD] and ulcerative colitis [UC]) where intestinal microbes are recognized to play an etiologic role. However, it is not known if transient fiber consumption during critical developmental periods may prevent consecutive intestinal inflammation. The incidence of IBD peaks in young adulthood indicating that pediatric environmental exposures may be important in the etiology of this disease group. We studied the effects of transient dietary cellulose supplementation on dextran sulfate sodium (DSS) colitis susceptibility during the pediatric period in mice. Cellulose supplementation stimulated substantial shifts in the colonic mucosal microbiome. Several bacterial taxa decreased in relative abundance (e.g., Coriobacteriaceae [p = 0.001]), and other taxa increased in abundance (e.g., Peptostreptococcaceae [p = 0.008] and Clostridiaceae [p = 0.048]). Some of these shifts persisted for 10 days following the cessation of cellulose supplementation. The changes in the gut microbiome were associated with transient trophic and anticolitic effects 10 days following the cessation of a cellulose-enriched diet, but these changes diminished by 40 days following reversal to a low cellulose diet. These findings emphasize the transient protective effect of dietary cellulose in the mammalian large bowel and highlight the potential role of dietary fibers in amelioration of intestinal inflammation. C1 [Nagy-Szakal, Dorottya; Kellermayer, Richard] Baylor Coll Med, Dept Pediat, Sect Pediat Gastroenterol, Houston, TX 77030 USA. [Nagy-Szakal, Dorottya; Smith, C. Wayne; Kellermayer, Richard] ARS, USDA, Childrens Nutr Res Ctr, Houston, TX USA. [Hollister, Emily B.; Luna, Ruth Ann; Szigeti, Reka; Versalovic, James] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA. [Hollister, Emily B.; Luna, Ruth Ann; Smith, C. Wayne; Versalovic, James; Kellermayer, Richard] Texas Childrens Hosp, Houston, TX 77030 USA. [Tatevian, Nina] Univ Texas Hlth Sci Ctr, Dept Pathol & Lab Med, Houston, TX USA. RP Kellermayer, R (reprint author), Baylor Coll Med, Dept Pediat, Sect Pediat Gastroenterol, Houston, TX 77030 USA. EM kellerma@bcm.edu FU Broad Medical Research Program, the Broad Foundation [IBD-0252]; Child Health Research Career Development Agency of the Baylor College of Medicine; Crohn's and Colitis Foundation of America-Children's Digestive Health and Nutrition Foundation/North American Society of Pediatric Gastroenterology Hepatology and Nutrition, (CCFA) [2426]; United States Department of Agriculture [6250-51000-046] FX This work was supported in part by the Broad Medical Research Program, the Broad Foundation (IBD-0252); the Child Health Research Career Development Agency of the Baylor College of Medicine; the Crohn's and Colitis Foundation of America-Children's Digestive Health and Nutrition Foundation/North American Society of Pediatric Gastroenterology Hepatology and Nutrition, (CCFA Ref #2426); the United States Department of Agriculture 6250-51000-046. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 9 Z9 9 U1 2 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 20 PY 2013 VL 8 IS 2 AR e56685 DI 10.1371/journal.pone.0056685 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 093IE UT WOS:000315184200141 PM 23437211 ER PT J AU Wang, Y Fu, TJ Howard, A Kothary, MH McHugh, TH Zhang, YZ AF Wang, Yang Fu, Tong-Jen Howard, Andrew Kothary, Mahendra H. McHugh, Tara H. Zhang, Yuzhu TI Crystal Structure of Peanut (Arachis hypogaea) Allergen Ara h 5 SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Peanut allergy; allergen stability; X-ray crystallography; Ara h 5; profilin ID IGE-BINDING EPITOPES; FOOD ALLERGY; CROSS-REACTIVITY; ALIGNMENT ALGORITHM; MUTATIONAL ANALYSIS; MOLECULAR GRAPHICS; TRYPSIN-INHIBITOR; MAJOR ALLERGEN; PROFILIN; PROTEIN AB Profilins from numerous species are known to be allergens, including food allergens, such as peanut (Arachis hypogaea) allergen Ara h 5, and pollen allergens, such as birch allergen Bet v 2. Patients with pollen allergy can also cross-react to peanut. Structural characterization of allergens will allow a better understanding of the allergenicity of food allergens and their cross-reactivities. The three-dimensional structures of most known food allergens remain to be elucidated. Here, we report the first crystallographic study of a food allergen in the profilin family. The structure of peanut allergen Ara h 5 was determined, and the resolution of the final refined structure was 1.1 angstrom. Structure alignment revealed that Ara h 5 is more similar to Bet v 2 than to Hey b 8, although sequence alignment suggested that Ara h 5 is more closely related to Hev b 8 than to Bet v 2, indicating that homology-model-based prediction of immunoglobulin E epitopes needs to be interpreted with caution. C1 [Wang, Yang; Howard, Andrew; Zhang, Yuzhu] IIT, Dept Biol & Chem Sci, Chicago, IL 60616 USA. [Fu, Tong-Jen] US FDA, Ctr Food Safety & Appl Nutr, Bedford Pk, IL 60501 USA. [Kothary, Mahendra H.] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD 20708 USA. [McHugh, Tara H.; Zhang, Yuzhu] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhang, YZ (reprint author), IIT, Dept Biol & Chem Sci, 3101 South Dearborn St, Chicago, IL 60616 USA. EM yuzhu.zhang@ars.usda.gov RI Zhang, Yuzhu/A-7109-2009 OI Zhang, Yuzhu/0000-0001-7882-5692 FU Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy [W-31-109-Eng-38]; U.S. Food and Drug Administration [5U01FD003801]; Institute for Food Safety and Health, Illinois Institute of Technology [5U01FD003801] FX X-ray diffraction data were collected at Southeast Regional Collaborative Access Team (SER-CAT) 22-ID beamline at the APS, Argonne National Laboratory. Use of the APS was supported by the Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy, under Contract W-31-109-Eng-38. This work was partially supported by Cooperative Agreement 5U01FD003801 between the U.S. Food and Drug Administration and Institute for Food Safety and Health, Illinois Institute of Technology. NR 44 TC 10 Z9 10 U1 0 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 20 PY 2013 VL 61 IS 7 BP 1573 EP 1578 DI 10.1021/jf303861p PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 095HV UT WOS:000315326200020 PM 23350842 ER PT J AU Jiang, WX Wang, ZH Beier, RC Jiang, HY Wu, YN Shen, JZ AF Jiang, Wenxiao Wang, Zhanhui Beier, Ross C. Jiang, Haiyang Wu, Yongning Shen, Jianzhong TI Simultaneous Determination of 13 Fluoroquinolone and 22 Sulfonamide Residues in Milk by a Dual-Colorimetric Enzyme-Linked Immunosorbent Assay SO ANALYTICAL CHEMISTRY LA English DT Article ID BROAD-SPECIFICITY ANTIBODIES; EDIBLE ANIMAL PRODUCTS; ANTIBIOTIC-RESIDUES; FOOD SAMPLES; LC-MS/MS; RESISTANCE; ELISA; IMMUNOASSAY; MECHANISMS AB Enzyme-linked immunosorbent assays (ELISAs) usually focus on the detection of a single analyte or a single group of analytes, e.g., fluoroquinolones or sulfonamides. However, it is often necessary to simultaneously monitor two classes of antimicrobial residues in different food matrixes. In this paper, we describe a dual-colorimetric ELISA for the simultaneous detection of 13 fluoroquinolone and 22 sulfonamide residues. The limit of detection for fluoroquinolones and sulfonamides was 2.4 and 5.8 ng/mL, respectively. The developed immunoassay is suitable for high-throughput screening of these low-molecular weight contaminants. This is the first report where two different enzymes (alkaline phosphatase and horseradish peroxidase) were used in one immunoassay and together in a single well for simultaneous detection of multiple low-molecular weight chemical residues. C1 [Jiang, Wenxiao; Wang, Zhanhui; Jiang, Haiyang; Shen, Jianzhong] China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Beijing 100193, Peoples R China. [Beier, Ross C.] USDA, Food & Feed Safety Res Unit, So Plains Agr Res Ctr, ARS, College Stn, TX 77845 USA. [Wu, Yongning] China Natl Ctr Food Safety Risk Assessment, Beijing 100021, Peoples R China. RP Shen, JZ (reprint author), China Agr Univ, Coll Vet Med, Dept Pharmacol & Toxicol, Beijing 100193, Peoples R China. EM sjz@cau.edu.cn OI Wang, Zhanhui/0000-0002-0167-9559; Jiang, Wenxiao/0000-0003-2443-7648 FU State Key Program of the National Natural Science Foundation of China [30830082, 31172631, 21107104]; Special Fund for Agro-scientific Research in the Public Interest [201203040]; National Science and Technology Pillar Program [2012BAK17B16, 2011BAK10B01-02]; International Science and Technology Cooperation Program of China [2011DFR30470] FX This work was supported by the State Key Program of the National Natural Science Foundation of China (Nos. 30830082, 31172631, and 21107104), Special Fund for Agro-scientific Research in the Public Interest (No. 201203040), National Science and Technology Pillar Program (Nos. 2012BAK17B16 and 2011BAK10B01-02), and the International Science and Technology Cooperation Program of China (No. 2011DFR30470). NR 29 TC 43 Z9 46 U1 4 U2 121 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD FEB 19 PY 2013 VL 85 IS 4 BP 1995 EP 1999 DI 10.1021/ac303606h PG 5 WC Chemistry, Analytical SC Chemistry GA 095IC UT WOS:000315326900006 PM 23347187 ER PT J AU Andorf, CM Honavar, V Sen, TZ AF Andorf, Carson M. Honavar, Vasant Sen, Taner Z. TI Predicting the Binding Patterns of Hub Proteins: A Study Using Yeast Protein Interaction Networks SO PLOS ONE LA English DT Article ID GDP-DISSOCIATION INHIBITOR; SACCHAROMYCES-CEREVISIAE; INTERACTION MAP; FAMILIES DATABASE; EVOLUTIONARY RATE; SCOP DATABASE; GENOME; MODULARITY; SEQUENCE; DESIGN AB Background: Protein-protein interactions are critical to elucidating the role played by individual proteins in important biological pathways. Of particular interest are hub proteins that can interact with large numbers of partners and often play essential roles in cellular control. Depending on the number of binding sites, protein hubs can be classified at a structural level as singlish-interface hubs (SIH) with one or two binding sites, or multiple-interface hubs (MIH) with three or more binding sites. In terms of kinetics, hub proteins can be classified as date hubs (i.e., interact with different partners at different times or locations) or party hubs (i.e., simultaneously interact with multiple partners). Methodology: Our approach works in 3 phases: Phase I classifies if a protein is likely to bind with another protein. Phase II determines if a protein-binding (PB) protein is a hub. Phase III classifies PB proteins as singlish-interface versus multiple-interface hubs and date versus party hubs. At each stage, we use sequence-based predictors trained using several standard machine learning techniques. Conclusions: Our method is able to predict whether a protein is a protein-binding protein with an accuracy of 94% and a correlation coefficient of 0.87; identify hubs from non-hubs with 100% accuracy for 30% of the data; distinguish date hubs/party hubs with 69% accuracy and area under ROC curve of 0.68; and SIH/MIH with 89% accuracy and area under ROC curve of 0.84. Because our method is based on sequence information alone, it can be used even in settings where reliable protein-protein interaction data or structures of protein-protein complexes are unavailable to obtain useful insights into the functional and evolutionary characteristics of proteins and their interactions. Availability: We provide a web server for our three-phase approach: http://hybsvm.gdcb.iastate.edu. C1 [Andorf, Carson M.; Honavar, Vasant] Iowa State Univ, Dept Comp Sci, Ames, IA USA. [Honavar, Vasant; Sen, Taner Z.] Iowa State Univ, Bioinformat & Computat Biol Program, Ames, IA 50011 USA. [Sen, Taner Z.] ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Sen, Taner Z.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA. RP Sen, TZ (reprint author), Iowa State Univ, Bioinformat & Computat Biol Program, Ames, IA 50011 USA. EM taner@iastate.edu RI Honavar, Vasant/K-9835-2015 OI Honavar, Vasant/0000-0001-5399-3489 FU National Institute of Health [GM066387]; Integrative Graduate Education and Research Training award [0504304]; National Science Foundation FX This work was funded in part by the National Institute of Health grant GM066387 to VH. The work of CMA was supported in part by a graduate fellowship funded by an Integrative Graduate Education and Research Training award 0504304. The work of VH while working at the National Science Foundation was supported by the National Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 82 TC 6 Z9 6 U1 0 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 19 PY 2013 VL 8 IS 2 AR e56833 DI 10.1371/journal.pone.0056833 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 093HR UT WOS:000315182800036 PM 23431393 ER PT J AU Figueroa, M Alderman, S Garvin, DF Pfender, WF AF Figueroa, Melania Alderman, Stephen Garvin, David F. Pfender, William F. TI Infection of Brachypodium distachyon by Formae Speciales of Puccinia graminis: Early Infection Events and Host-Pathogen Incompatibility SO PLOS ONE LA English DT Article ID GENETIC-LINKAGE MAP; F-SP TRITICI; NONHOST RESISTANCE; MODEL SYSTEM; STEM RUST; TALL FESCUE; HYPERSENSITIVE RESPONSE; PERENNIAL RYEGRASS; DISEASE RESISTANCE; GRASS AB Puccinia graminis causes stem rust, a serious disease of cereals and forage grasses. Important formae speciales of P. graminis and their typical hosts are P. graminis f. sp. tritici (Pg-tr) in wheat and barley, P. graminis f. sp. lolii (Pg-lo) in perennial ryegrass and tall fescue, and P. graminis f. sp. phlei-pratensis (Pg-pp) in timothy grass. Brachypodium distachyon is an emerging genetic model to study fungal disease resistance in cereals and temperate grasses. We characterized the P. graminis-Brachypodium pathosystem to evaluate its potential for investigating incompatibility and non-host resistance to P. graminis. Inoculation of eight Brachypodium inbred lines with Pg-tr, Pg-lo or Pg-pp resulted in sporulating lesions later accompanied by necrosis. Histological analysis of early infection events in one Brachypodium inbred line (Bd1-1) indicated that Pg-lo and Pg-pp were markedly more efficient than Pg-tr at establishing a biotrophic interaction. Formation of appressoria was completed (60-70% of germinated spores) by 12 h post-inoculation (hpi) under dark and wet conditions, and after 4 h of subsequent light exposure fungal penetration structures (penetration peg, substomatal vesicle and primary infection hyphae) had developed. Brachypodium Bd1-1 exhibited pre-haustorial resistance to Pg-tr, i.e. infection usually stopped at appressorial formation. By 68 hpi, only 0.3% and 0.7% of the Pg-tr urediniospores developed haustoria and colonies, respectively. In contrast, development of advanced infection structures by Pg-lo and Pg-pp was significantly more common; however, Brachypodium displayed post-haustorial resistance to these isolates. By 68 hpi the percentage of urediniospores that only develop a haustorium mother cell or haustorium in Pg-lo and Pg-pp reached 8% and 5%, respectively. The formation of colonies reached 14% and 13%, respectively. We conclude that Brachypodium is an apt grass model to study the molecular and genetic components of incompatiblity and non-host resistance to P. graminis. C1 [Figueroa, Melania; Alderman, Stephen; Pfender, William F.] ARS, Forage Seed & Cereal Res Unit, USDA, Corvallis, OR USA. [Garvin, David F.] ARS, Plant Sci Res Unit, USDA, St Paul, MN USA. [Garvin, David F.] ARS, Dept Agron & Plant Genet, USDA, St Paul, MN USA. [Pfender, William F.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Pfender, WF (reprint author), ARS, Forage Seed & Cereal Res Unit, USDA, Corvallis, OR USA. EM pfenderw@onid.orst.edu FU USDA Agricultural Research Service [5358-22000-031-00D] FX Research was supported by basic operating funds of the USDA Agricultural Research Service, Project 5358-22000-031-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 18 Z9 19 U1 1 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 18 PY 2013 VL 8 IS 2 AR e56857 DI 10.1371/journal.pone.0056857 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 092YP UT WOS:000315159200075 PM 23441218 ER PT J AU Edwards, JF Dubey, JP AF Edwards, John F. Dubey, J. P. TI Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T-gondii from an aborted lamb from a chronically infected ewe SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Sheep; Abortion; Transmission; Epidemiology; Texas ID CENTRAL UNITED-STATES; VERTICAL TRANSMISSION; OVINE TOXOPLASMOSIS; CONGENITAL TOXOPLASMOSIS; MICROSATELLITE ANALYSIS; HUMAN CONSUMPTION; CHAROLLAIS SHEEP; HIGH PREVALENCE; LINEAGES; DIVERSE AB Sheep are commonly infected with the protozoan parasite, Toxoplasmagondii. Infection may cause early embryonic death and resorption, fetal death and mummification, abortion, stillbirth, and neonatal death. Most sheep acquire T. gondii infection after birth. Recent studies reported that congenital ovine transmission of T. gondii may be more common than previously believed, but these findings are solely based on PCR data and require confirmation using other techniques to verify the findings. In the present study, during the lambing season of 2005 a toxoplasmosis abortion storm occurred in a flock of purebred Suffolk ewes on a farm in Texas. Only 14 healthy lambs were born, and 38 abortuses, mummies and weak or stillborn lambs were delivered. Another 15 fetuses identified by ultrasound were presumably resorbed or were aborted undetected. Antibodies to T. gondii were found in 37 (94.8%) of the 39 ewes and 30 of them had high titers (1:3200 or higher) when tested in the modified agglutination test (MAT). In the 2006 lambing season, two (both with MAT titers of >= 3200 in 2005) of 26 ewes delivered T. gondii infected lambs. T. gondii tissue cysts were found histologically in lesions of encephalitis in a lamb from one ewe and viable T. gondii (designated TgShUs55) was isolated from the brain and heart of a lamb from the second ewe. TheTgShUs55 had an atypical genotype using 10 PCR-RFLP markers, and was 100% lethal for Swiss Webster mice, irrespective of the dose or the stage of the parasite inoculated. In subsequent seasons, the ewes lambed normally. The results of the present study support the hypothesis that most sheep that have aborted due to T. gondii develop protection against future toxoplasmosis induced abortion, but the protection is not absolute. (c) 2012 Elsevier B.V. All rights reserved. C1 [Edwards, John F.] Texas A&M Univ, Dept Vet Pathobiol, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 45 TC 23 Z9 24 U1 0 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD FEB 18 PY 2013 VL 192 IS 1-3 BP 129 EP 136 DI 10.1016/j.vetpar.2012.09.037 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 078DP UT WOS:000314078900015 PM 23099088 ER PT J AU Katiki, LM Ferreira, JFS Gonzalez, JM Zajac, AM Lindsay, DS Chagas, ACS Amarante, AFT AF Katiki, Luciana M. Ferreira, Jorge F. S. Gonzalez, Javier M. Zajac, Anne M. Lindsay, David S. Chagas, Ana Carolina S. Amarante, Alessandro F. T. TI Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity SO VETERINARY PARASITOLOGY LA English DT Article DE Hydrolyzable tannins; Condensed tannins; Anthelmintic plants; Tree tannins; C. elegans; ORAC; Antioxidant capacity; Total phenolics; Nematodes; Small ruminants ID INDICA A. JUSS; IN-VITRO; AZADIRACHTA-INDICA; PARASITIC NEMATODES; GALLIC ACID; SHEEP; LEAVES; ANTIBACTERIAL; ELLAGITANNINS; METHANOLYSIS AB Although tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0 mg/mL to 25 mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24h were determined and compared to the veterinary anthelmintic levamisole (8 mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum, Rosa multi-flora, and Quercus alba, while Rhus typhina had both HT and CT. The extracts with the lowest LC50 and LC90 concentrations, respectively, in the C elegans assay were Q. alba (0.75 and 1.06 mg/mL), R. typhina collected in 2007 (0.65 and 2.74 mg/mL), A. rubrum (1.03 and 5.54 mg/mL), and R. multiflora (2.14 and 8.70 mg/mL). At the doses of 20 and 25 mg/mL, HT-rich, or both CT- and HT-rich, extracts were significantly more lethal to adult C. elegans than extracts containing only CT. All extracts were high in antioxidant capacity, with ORAC values ranging from 1800 mu moles to 4651 mu moles of trolox equivalents/g, but ORAC did not correlate with anthelmintic activity. The total phenolics test had a positive and highly significant (r = 0.826, p <= 0.01) correlation with total hydrolyzable tannins. Plants used in this research are naturalized to the Appalachian edaphoclimatic conditions, but occur in temperate climate areas worldwide. They represent a rich, renewable, and unexplored source of tannins and antioxidants for grazing ruminants, whereas conventional CT-rich forages, such as L. cuneata, may be hard to establish and adapt to areas with temperate climate. Due to their high in vitro anthelmintic activity, antioxidant capacity, and their adaptability to non-arable lands, Q. alba, R. typhina, A. rubrum, and R. multiflora have a high potential to improve the health of grazing animals and must have their anthelmintic effects confirmed in vivo in both sheep and goats. Published by Elsevier B.V. C1 [Katiki, Luciana M.] Inst Zootecnia SAA APTA, BR-13460000 Nova Odessa, SP, Brazil. [Ferreira, Jorge F. S.; Gonzalez, Javier M.] ARS, Appalachian Farming Syst Res Ctr, USDA, Beaver, WV 25813 USA. [Zajac, Anne M.; Lindsay, David S.] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24061 USA. [Chagas, Ana Carolina S.] Embrapa Pecuaria Sudeste, Sao Carlos, SP, Brazil. [Amarante, Alessandro F. T.] Univ Estadual Paulista, UNESP, Dept Parasitol, Botucatu, SP, Brazil. RP Ferreira, JFS (reprint author), US Salin Lab, 450W Big Springs Rd, Riverside, CA 92507 USA. EM Jorge.Ferreira@ars.usda.gov RI Amarante, Alessandro/C-8773-2012; Lindsay, David/G-8891-2016; Chagas, A. C. S./H-7751-2013; OI Amarante, Alessandro/0000-0003-3995-5501; Lindsay, David/0000-0002-0592-8321; Chagas, A. C. S./0000-0003-3939-0088; Ferreira, Jorge F.S./0000-0003-4550-6761 FU CAPES; Appalachian Farming Systems Research Center, AFSRC (USDA-ARS); Virginia Polytechnical Institute FX We acknowledge the financial support from CAPES and the specific cooperative agreement (SCA) between the Appalachian Farming Systems Research Center, AFSRC (USDA-ARS) and the Virginia Polytechnical Institute. We also appreciate the technical assistances of the USDA-ARS technical staff for their valuable assistance with the initial C. elegans cultures (Mrs. Carol Masler, Nematology Lab-Beltsville), tannin extractions (Mr. Barry Harter, AFSRC, Beaver, WV), and with the ORAC analysis (Mr. Robert Arnold, AFSRC, Beaver, WV). Acknowledgements are also due to Dr. Donna Ford-Werntz, curator of the West Virginia University herbarium, for the taxonomic confirmation of the species used in this study and to Mr. John Phillips (Statistician General, Wyndmore, PA) for his sound advice and help with statistical analyses. NR 62 TC 15 Z9 16 U1 2 U2 91 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD FEB 18 PY 2013 VL 192 IS 1-3 BP 218 EP 227 DI 10.1016/j.vetpar.2012.09.030 PG 10 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 078DP UT WOS:000314078900026 PM 23102761 ER PT J AU Potter, TL Bosch, DD Dieppa, A Whitall, DR Strickland, TC AF Potter, Thomas L. Bosch, David D. Dieppa, Angel Whitall, David R. Strickland, Timothy C. TI Atrazine fate and transport within the coastal zone in southeastern Puerto Rico SO MARINE POLLUTION BULLETIN LA English DT Article DE Herbicide; Soil; Dissipation; Runoff; Groundwater; Tropical; Storm ID GREAT-BARRIER-REEF; SURFACE-WATER; HERBICIDE; DEGRADATION; DISSIPATION; PESTICIDES; SOILS; DEETHYLATRAZINE; IMPACT AB Agrichemical transport to coastal waters may have adverse ecological impact. This work examined atrazine fate and transport in a field adjacent to Puerto Rico's Jobos Bay National Estuarine Research Reserve. The herbicide's use was linked to residue detection in shallow groundwater and movement toward the estuary; however, data indicated that transport via this pathway was small. In contrast, surface runoff as tropical storm systems moved through the area appeared to have high potential for atrazine transport. In this case, transport to the estuary was limited by runoff event timing relative to atrazine application and very rapid atrazine dissipation (DT50 = 1-3 days) in field soil. Soil incubation studies showed that accelerated degradation conditions had developed in the field due to repeated atrazine treatment. To improve weed management, atrazine replacement with other herbicide(s) is recommended. Use of products that have greater soil persistence may increase runoff risk. Published by Elsevier Ltd. C1 [Potter, Thomas L.; Bosch, David D.; Strickland, Timothy C.] USDA ARS, Southeast Watershed Lab, Tifton, GA 31793 USA. [Dieppa, Angel] Jobos Bay Natl Estuarine Res Reserve, Aguirre, PR 00704 USA. [Whitall, David R.] NOAA Natl Ctr Coastal Ocean Sci, Silver Spring, MD 20910 USA. RP Potter, TL (reprint author), USDA ARS, Southeast Watershed Lab, Tifton, GA 31793 USA. EM Tom.Potter@ars.usda.gov OI Strickland, Timothy/0000-0001-6889-503X FU USDA-NRCS through the CEAP program FX USDA-ARS employees, Margie Whittle, Sally Belflower, Laura Marshall, Lorine Lewis, and Coby Smith and Jacqueline Vega-Perez, Gerson Ardila-Sierra, and David Sotomayor-Ramirez, University of Puerto Rico - Mayaguez, provided essential field and laboratory support. Thanks also to the JBNERR-DNER program staff for their support and collaboration, to Jose M. Rodriguez, USGS, Ellis Benham and Edwin Mas, USDA-NRCS, the Puerto Rico Land Authority and Efrain Ayala-Benitez, Salinas Silage Inc. USDA-NRCS provided funds for this research through the CEAP program. NR 38 TC 4 Z9 4 U1 2 U2 24 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0025-326X EI 1879-3363 J9 MAR POLLUT BULL JI Mar. Pollut. Bull. PD FEB 15 PY 2013 VL 67 IS 1-2 BP 36 EP 44 DI 10.1016/j.marpolbul.2012.12.004 PG 9 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 111MV UT WOS:000316526300018 PM 23321597 ER PT J AU Moreira, FKV Pedro, DCA Glenn, GM Marconcini, JM Mattoso, LHC AF Moreira, Francys K. V. Pedro, Daniel C. A. Glenn, Gregory M. Marconcini, Jose M. Mattoso, Luiz H. C. TI Brucite nanoplates reinforced starch bionanocomposites SO CARBOHYDRATE POLYMERS LA English DT Article DE Biodegradable plastics; Starch; Brucite nanoplates; Bionanocomposites; Mechanical reinforcement ID MAGNESIUM-HYDROXIDE NANOPARTICLES; THERMOPLASTIC STARCH; PLASTICIZED STARCH; NANOCOMPOSITES; COMPOSITES; POLYMERS; OXIDE; SPECTROSCOPY; MG(OH)(2); STATE AB In this paper the mechanical reinforcement of nano-sized brucite, Mg(OH)(2) in a series of bionanocomposite films based on starch was investigated. Brucite nanoplates with an aspect ratio of 9.25 were synthesized by wet precipitation and incorporated into starch matrices at different concentrations (0-7.5 wt.%). Scanning electron microscopy revealed a high degree of nanoplate dispersion within the starch bionanocomposites and good interfacial adhesion between the filler and matrix. The brucite nanoplates formed agglomerates at high concentrations. The reinforcement factor values of the bionanocomposites were higher than the values predicted from the Halphin-Tsai model, which was attributed mainly to the high surface area of the nanoplates. Brucite (1 wt.%) nearly doubled the elastic modulus of starch films. Thermogravimetric analyses indicated some interaction between starch and the brucite that modified their decomposition profiles. Mechanical tests of glycerol plasticized bionanocomposites showed that the reinforcing efficiency of brucite remained high even at 10 wt.% and 20 wt.% of plasticizer. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Moreira, Francys K. V.; Pedro, Daniel C. A.; Marconcini, Jose M.; Mattoso, Luiz H. C.] Embrapa Instrumentacao CNPDIA, LNNA, BR-13560970 Sao Carlos, SP, Brazil. [Moreira, Francys K. V.] Univ Fed Sao Carlos, Programa Posgrad Ciencia & Engn Mat PPG CEM, BR-13560 Sao Carlos, SP, Brazil. [Pedro, Daniel C. A.] Univ Fed Sao Carlos, Dept Engn Mat DEMA, BR-13560 Sao Carlos, SP, Brazil. [Glenn, Gregory M.] ARS, Western Reg Res Ctr WRRC, USDA, Albany, CA USA. RP Mattoso, LHC (reprint author), Embrapa Instrumentacao CNPDIA, LNNA, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP, Brazil. EM mattoso@cnpdia.embrapa.br RI Marconcini, Jose/D-9702-2013; Moreira, Francys/G-5271-2015; Mattoso, Luiz H C/D-2794-2016 OI Moreira, Francys/0000-0001-6368-3177; Mattoso, Luiz H C/0000-0001-7586-1014 FU Embrapa; FINEP/MCT; CNPq; CAPES; Labex Embrapa/ARS; FAPESP agency [2010/11584-5] FX The authors are grateful to Embrapa, FINEP/MCT, CNPq, CAPES and Labex Embrapa/ARS for their financial support. The authors thank to Mr. Vitor Anibal do Sacramento Mendes from LCE/DEMA-UFSCar for his helpful assistance with the SEM characterizations and to Mr. Luiz Francisco de Matteo Ferraz from CNPDIA for has carried out the WAXS measurements. F.K.V. Moreira is grateful to FAPESP agency (Proc. no. 2010/11584-5) for his Ph.D. scholarship. This study is a part of the researches conducted by the Rede de Nanotecnologia Aplicada ao Agronegocio (Rede Agronano), Brazil. NR 39 TC 13 Z9 13 U1 6 U2 41 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD FEB 15 PY 2013 VL 92 IS 2 BP 1743 EP 1751 DI 10.1016/j.carbpol.2012.11.019 PG 9 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 099JI UT WOS:000315616900101 PM 23399215 ER PT J AU Fishman, ML Chau, HK Qi, PX Hotchkiss, AT Yadav, MP AF Fishman, Marshall L. Chau, Hoa K. Qi, Phoebe X. Hotchkiss, Arland T., Jr. Yadav, Madhav P. TI Physico-chemical characterization of protein-associated polysaccharides extracted from sugar beet pulp SO CARBOHYDRATE POLYMERS LA English DT Article DE Pectin; Alkaline soluble polysaccharides; Sodium carboxy methyl cellulose; Protein; Chemical composition; Molecular characterization; HPSEC ID FERULIC ACID; PECTIN AB We have solubilized and separated polysaccharides from sugar beet pulp (SBP) into three fractions with steam assisted flash extraction (SAFE). For pectin, recovery ranged from 8 to 14%, degree of methy-esterification 66-73%, crude protein 1.3-1.7%, M-w 262-318 kDa, eta(w) 0.22-0.23 dL/g, Rg(z) 36-39 nm and Rh-z 41-42 nm. For alkaline soluble polysaccharides, (ASP I) recovery ranged from 4.0 to 6.5%, crude protein 1.2-4.8%, weight average molar mass (M-w) 66-68 kDa, weight average intrinsic viscosity (eta(w)) 0.27-0.30 dL/g, z-average radius of gyration (Rg(z)) 25-29 nm and z-average hydrated radius (Rh-z) 10-11 nm. ASP II recovery ranged from 2.0 to 8.6%, crude protein 1.2-4.8%, M-w 299-339 kDa, eta(w) 0.22-0.33 dL/g, Rg(z) 33-34nm and Rh-z 30-34 nm. Recovery of the residue mainly cellulose, ranged from 20.3 to 22.3%. The cellulose in this fraction was converted to carboxymethyl cellulose (CMC). The CMC fraction contained 0.33-0.43 crude protein and had an M-w ranging from 127 to 263 kDa, eta(w) 3.6-8.0 dL/g, Rg(z) 35-45 nm and Rh-z 27-40 nm. Published by Elsevier Ltd. C1 [Fishman, Marshall L.; Chau, Hoa K.; Qi, Phoebe X.; Hotchkiss, Arland T., Jr.] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Yadav, Madhav P.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Fishman, ML (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM marshall.fishman@ars.usda.gov FU SAFE FX We thank Andre White for technical assistance in determining carbohydrate compositional analysis, Edward Wickham for assistance in obtaining infrared spectra, Robyn Moten and Michael Kurantz for assistance in determining protein composition, Neil Goldberg and Jhanel Wilson for their assistance and support in the SAFE of sugar beet pulp, Kenneth Todd in the schematic drawing of the SAFE vessel and Gerard Senske for preparing sugar beet pulp for extraction. NR 19 TC 6 Z9 6 U1 4 U2 46 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD FEB 15 PY 2013 VL 92 IS 2 BP 2257 EP 2266 DI 10.1016/j.carbpol.2012.12.001 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 099JI UT WOS:000315616900172 PM 23399286 ER PT J AU Guan, W Shao, J Singh, R Davis, RE Zhao, TC Huang, Q AF Guan, Wei Shao, Jonathan Singh, Raghuwinder Davis, Robert E. Zhao, Tingchang Huang, Qi TI A TaqMan-based real time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Xylella fastidiosa; Taq Man probes; Real-time quantitative PCR; Bacterial leaf scorch in oleander ID POLYMERASE-CHAIN-REACTION; PIERCES-DISEASE STRAINS; CITRUS VARIEGATED CHLOROSIS; SOUTHERN CALIFORNIA; GENOME SEQUENCES; LANDSCAPE TREES; 1ST REPORT; IDENTIFICATION; NOV AB A TaqMan-based real-time PCR assay was developed for specific detection of strains of X. fastidiosa causing oleander leaf scorch. The assay uses primers WG-OLS-F1 and WG-OLS-R1 and the fluorescent probe WG-OLS-P1, designed based on unique sequences found only in the genome of oleander strain Ann1. The assay is specific, allowing detection of only oleander-infecting strains, not other strains of X. fastidiosa nor other plant-associated bacteria tested. The assay is also sensitive, with a detection limit of 10.4 fg DNA of X. fastidiosa per reaction in vitro and in planta. The assay can also be applied to detect low numbers of X. fastidiosa in insect samples, or further developed into a multiplex real-time PCR assay to simultaneously detect and distinguish diverse strains of X. fastidiosa that may occupy the same hosts or insect vectors. Specific and sensitive detection and quantification of oleander strains of X. fastidiosa should be useful for disease diagnosis, epidemiological studies, management of oleander leaf scorch disease, and resistance screening for oleander shrubs. Published by Elsevier B.V. C1 [Guan, Wei; Huang, Qi] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Shao, Jonathan; Davis, Robert E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Singh, Raghuwinder] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Guan, Wei; Zhao, Tingchang] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China. RP Huang, Q (reprint author), USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. EM qi.huang@ars.usda.gov NR 32 TC 2 Z9 3 U1 3 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 J9 J MICROBIOL METH JI J. Microbiol. Methods PD FEB 15 PY 2013 VL 92 IS 2 BP 108 EP 112 DI 10.1016/j.mimet.2012.11.008 PG 5 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 095YM UT WOS:000315370900003 PM 23165115 ER PT J AU Clotilde, LM Bernard, C Salvador, A Lin, A Lauzon, CR Muldoon, M Xu, YC Lindpaintner, K Carter, JM AF Clotilde, Laurie M. Bernard, Clay Salvador, Alexandra Lin, Andrew Lauzon, Carol R. Muldoon, Mark Xu, Yichun Lindpaintner, Klaus Carter, J. Mark TI A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Escherichia coli; Serotyping; Multiplex; Immunoassay; Antisera; Luminex ID HEMOLYTIC-UREMIC SYNDROME; UNITED-STATES; NON-O157; INFECTIONS; O157; O111; IDENTIFICATION; PATHOGENS; OUTBREAK; SAMPLES AB Serotyping of Shiga toxin-producing Escherichia coli (STEC) has been contingent upon the availability of antisera. Here we describe a 7-plex microbead-based immunoassay to simultaneously serotype seven STECs (i.e., belonging to serogroups O26, O45,O103, O111, O121, O145, and O157) by the Luminex xMAP (R) technology. This technology presents many advantages: Its multiplexed format (up to 100 analytes) saves time, reagents, and test sample, and many regulatory agencies currently utilize this platform for other assays. In this study, a total of seventy-nine STEC strains belonging to the 7 different serogroups of interest were tested. These strains had been previously serotyped and their serogroup was confirmed by PCR. Except for one strain belonging to the O111 serogroup, nearly all strains (i.e., 98.7%; 78/79) were correctly identified on the Bio-Plex 100 instrument in less than 4 h. This newly developed microbead-based immunoassay could be extended to include other STEC serogroups, virulence factors, and/or bacterial species. Published by Elsevier B.V. C1 [Clotilde, Laurie M.; Lin, Andrew] US FDA, San Francisco Dist Lab, Alameda, CA 94502 USA. [Bernard, Clay; Salvador, Alexandra; Carter, J. Mark] ARS, USDA, Albany, CA 94710 USA. [Lauzon, Carol R.] Calif State Univ Hayward, Dept Biol Sci, Hayward, CA 94542 USA. [Muldoon, Mark] Romer Labs Technol Inc, Newark, DE 19713 USA. [Xu, Yichun; Lindpaintner, Klaus] Strateg Diagnost Inc SDIX, Newark, DE 19702 USA. RP Carter, JM (reprint author), 800 Buchanan St, Albany, CA 94710 USA. EM j.mark.carter@ars.usda.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 NR 29 TC 6 Z9 6 U1 0 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 J9 J MICROBIOL METH JI J. Microbiol. Methods PD FEB 15 PY 2013 VL 92 IS 2 BP 226 EP 230 DI 10.1016/j.mimet.2012.11.023 PG 5 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 095YM UT WOS:000315370900024 PM 23228591 ER PT J AU Wang, LL Hunt, ER Qu, JJ Hao, XJ Daughtry, CST AF Wang, Lingli Hunt, E. Raymond, Jr. Qu, John J. Hao, Xianjun Daughtry, Craig S. T. TI Remote sensing of fuel moisture content from ratios of narrow-band vegetation water and dry-matter indices SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Fuel moisture content; Spectral reflectance; Normalized Dry Matter Index; Normalized Difference Infrared Index; PROSPECT; SAIL ID CANOPY REFLECTANCE; MODEL INVERSION; BOREAL REGIONS; CROP RESIDUES; SENSOR DATA; LEAF; SOIL; SIMULATION; THICKNESS; NITROGEN AB Fuel moisture content (FMC) is an important variable for predicting the occurrence and spread of wildfire. Because FMC is calculated from the ratio of canopy water content to dry-matter content, we hypothesized that FMC may be estimated by remote sensing with a ratio of a vegetation water index to a vegetation dry-matter index. Four vegetation water indices, six dry-matter indices, and the resulting water/dry-matter index ratios were calculated using simulated leaf reflectances from the PROSPECT model. Two water indices, the Normalized Difference Infrared Index (NDII) and the Normalized Difference Water Index (NDWI), were more correlated with leaf water content than with FMC, and were not correlated with leaf dry-matter content. Two dry-matter indices, the Normalized Dry Matter Index (NDMI) and a recent index (unnamed) were correlated to leaf dry matter content, were inversely correlated with FMC, and were not correlated with water content. Ratios of these water indices and these dry-matter indices were highly and consistently correlated with FMC. Ratios of other water indices with other dry-matter indices were not consistently correlated with FMC. The ratio of NDII with NDMI was strongly related to FMC by a quadratic polynomial equation with an R-2 of 0.947. Spectral reflectance data were acquired for single leaves and leaf stacks of Quercus alba, Acer rubrum, and Zea mays; the relationship between FMC and NDII/NDMI had an R-2 of 0.853 and was almost identical to the equation from the PROSPECT model simulations. For the SAIL model simulations, the relationship between NDII/NDMI and FMC at the canopy scale had an R-2 of 0.900, but the quadratic polynomial equation differed from the equations determined from the PROSPECT simulations and spectral reflectance data. NDMI requires narrow-band sensors to measure the effect of dry matter on reflectance at 1722 nm whereas NDII may be determined with many different sensors. Therefore, monitoring FMC with NDII/NDMI requires either a new sensor or a combination of two sensors, one with high temporal resolution for monitoring water content and one with high spectral resolution for estimating dry-matter content. Published by Elsevier Inc. C1 [Wang, Lingli; Qu, John J.; Hao, Xianjun] George Mason Univ, Coll Sci, Dept Geog & GeoInformat Sci, Environm Sci & Technol Ctr, Fairfax, VA 22030 USA. [Hunt, E. Raymond, Jr.; Daughtry, Craig S. T.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Hunt, ER (reprint author), ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. EM Raymond.Hunt@ars.usda.gov RI Hao, Xianjun/F-7253-2016; Hao, Xianjun/C-9543-2011 OI Hao, Xianjun/0000-0002-8186-6839; Hao, Xianjun/0000-0002-8186-6839 NR 47 TC 16 Z9 18 U1 1 U2 60 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB 15 PY 2013 VL 129 BP 103 EP 110 DI 10.1016/j.rse.2012.10.027 PG 8 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 095BA UT WOS:000315308300009 ER PT J AU Seto, D Clements, CB Heilman, WE AF Seto, Daisuke Clements, Craig B. Heilman, Warren E. TI Turbulence spectra measured during fire front passage SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Wildland fire; Turbulence; Spectra; Fire-atmosphere interactions ID SURFACE-LAYER TURBULENCE; FREE-BURNING FIRES; BOUNDARY-LAYER; ATMOSPHERIC-TURBULENCE; WAVELET TRANSFORMS; DISSIPATION RATES; COMPLEX TERRAIN; STREET CANYON; GRASS FIRES; WIND AB Four field experiments were conducted over various fuel and terrain to investigate turbulence generation during the passage of wildland fire fronts. Our results indicate an increase in horizontal mean winds and friction velocity, horizontal and vertical velocity variances as well as a decreased degree of anisotropy in TKE during fire front passage (FFP) due to fire-induced winds. Vertical velocity and temperature variances observed during FFP approached the local free convection prediction when represented as a function of stability parameter z/L under very unstable conditions. The results of our wavelet spectral analysis show increased energy in velocity and temperature spectra at high frequency during FFP for all four cases; we hypothesize this is caused by the shedding of small eddies generated from the fire front. Additionally, spectral energy of velocity components at low frequencies may be affected by cross-flow intensity, topography, presence of canopy layer, and degree of fire-atmosphere coupling. When the velocity spectra are normalized using the friction velocity u(*) following Monin-Obukhov scaling, the velocity spectra observed during the FFP collapsed into a fairly narrow band in the inertial subrange, suggesting that as far as inertial range is concerned, the friction velocity u(*) is a valid scaling parameter that can be used for wildfire application. When the temperature spectra are normalized by T-*, the temperature spectra observed during the FFP did not show any systematic behaviors predicted by the similarity scaling due to the extreme surface heating environment of fires. (c) 2012 Elsevier B.V. All rights reserved. C1 [Seto, Daisuke; Clements, Craig B.] San Jose State Univ, Dept Meteorol & Climate Sci, San Jose, CA 95192 USA. [Heilman, Warren E.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. RP Clements, CB (reprint author), San Jose State Univ, Dept Meteorol & Climate Sci, San Jose, CA 95192 USA. EM craig.clements@sjsu.edu FU Research Joint Venture Agreement with the USDA Forest Service - Northern Research Station [07-JV-11242300-073]; Joint Fire Science Program [09-1-04-2] FX The authors would like to first acknowledge the two anonymous reviewers for their suggestions and comments that greatly improved our analysis and manuscript. All experiments were supported by a Research Joint Venture Agreement with the USDA Forest Service - Northern Research Station (#07-JV-11242300-073). The sub-canopy experiments were supported by the Joint Fire Science Program (Award# 09-1-04-2). Dr. Tara Strand (Pacific Northwest Research Laboratory, USDA Forest Service) is acknowledged for her role as PI during the sub-canopy experiments. The authors thank the Cal Fire Santa Clara Unit (and in particular Battalion Chief Dave McLean) for conducting the prescribed burn in the valley location and for accommodating our research objectives. The Santa Clara County Department of Parks and Recreation is thanked for the special-use permit. The Contra Costa County Fire Department and other agencies involved in executing the planning and operations of the Wildfire 2010 training drill are acknowledged for accommodating our slope fire experiment. We thank Dennis Burns of the Pleasanton Fire Department for helping organize our research objectives into the burn plan. Travel support to participate in the IS4FIRES experiment is acknowledged from the Finnish Meteorological Institute, and in particular, Profs. David Schultz and M. Kumala. Janne Levula and Prof. Gerrit de Leeuw are thanked for help with the installation and the operation of our instrumentation at the Hyytiala Forestry Field Station. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the U.S. Department of Agriculture of any product or service. NR 56 TC 5 Z9 5 U1 0 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD FEB 15 PY 2013 VL 169 BP 195 EP 210 DI 10.1016/j.agrformet.2012.09.015 PG 16 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 078GT UT WOS:000314087400018 ER PT J AU Zychowski, KE Pohlenz, C Mays, T Romoser, A Hume, M Buentello, A Gatlin, DM Phillips, TD AF Zychowski, Katherine E. Pohlenz, Camilo Mays, Travis Romoser, Amelia Hume, Michael Buentello, Alejandro Gatlin, Delbert M., III Phillips, Timothy D. TI The effect of NovaSil dietary supplementation on the growth and health performance of Nile tilapia (Oreochromis niloticus) fed aflatoxin-B1 contaminated feed SO AQUACULTURE LA English DT Article DE Aflatoxin; Tilapia; Montmorillonite; Enterosorption; Clay ID LACTIC-ACID BACTERIA; X MORONE-SAXATILIS; RAINBOW-TROUT; CLAY INTERVENTION; HIGH-RISK; IN-VITRO; B-1; BINDING; RATS; PREVENTION AB The objective of this study was to evaluate the ability of NovaSil (NS) clay to sorb and mitigate the toxic effects of aflatoxin B-1 (AFB(1)) in Nile tilapia (Oreochromis niloticus). Growth performance, targeted innate immunological function, intestinal microbial community and histology were evaluated after feeding tilapia diets with or without AFB(1) and/or NS for 10 weeks. Aflatoxin B-1 at concentrations of 1.5 and 3.0 ppm significantly (P<0.05) decreased weight gain, feed efficiency, hepatosomatic index and macrophage extracellular superoxide anion production in tilapia, regardless of NS addition to the diet. The overall results regarding the efficacy of NS were mixed; however, there was a trend (P=0.157) towards AFB(1)-toxicity prevention in regards to macrophage extracellular superoxide anion production. Additionally, when 0.5 and 1% NS was included in diets containing 1.5 ppm AFB(1), total histopathological score was lowered; however, this protective effect was not evident when fish were exposed to 3.0 ppm AFB(1). Denaturing gradient gel electrophoresis was performed to assess the effects of both AFB(1) and NS on gut microbiota, but no significant differences were found among treatment groups. (C) 2012 Elsevier B.V. All rights reserved. C1 [Zychowski, Katherine E.; Mays, Travis; Romoser, Amelia; Phillips, Timothy D.] Texas A&M Univ, Coll Vet Med, College Stn, TX 77843 USA. [Pohlenz, Camilo; Buentello, Alejandro; Gatlin, Delbert M., III] Texas A&M Univ, Dept Wildlife & Fisheries, College Stn, TX 77843 USA. [Hume, Michael] ARS, USDA, So Plains Agr Res Inst, SPARC,Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Phillips, TD (reprint author), Texas A&M Univ, Coll Vet Med, TAMU 4458, College Stn, TX 77843 USA. EM tphillips@cvm.tamu.edu FU CONACyT [2010-020] FX The authors would like to thank Brian Ray, Aquacultural Research and Teaching Facility Manager, Texas A&M University and Charles Hernandez, Biological Science Lab Technician, USDA, College Station, TX. Additionally, we would like to thank Kelly Scribner, Nicole Mitchell, Kristal Brown and Sarah Elmore (Toxicology program), and Maritza Anguiano and Angie Peredo (Wildlife and Fisheries), Texas A&M University for their technical support in this project. Funding for this project was provided by CONACyT 2010-020. This research was conducted as a component of Ms. Zychowski's Ph.D. program in Toxicology through the Texas A&M University Veterinary Integrative Biosciences department. NR 57 TC 6 Z9 6 U1 0 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD FEB 15 PY 2013 VL 376 BP 117 EP 123 DI 10.1016/j.aquaculture.2012.11.020 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 071EK UT WOS:000313569100017 ER PT J AU Xu, DH Shoemaker, CA Zhang, QZ Klesius, PH AF Xu, De-Hai Shoemaker, Craig A. Zhang, Qizhong Klesius, Phillip H. TI Naturally infected channel catfish (Ictalurus punctatus) concurrently transmit Ichthyophthirius multifiliis and Edwardsiella ictaluri to naive channel catfish SO AQUACULTURE LA English DT Article DE Coinfection; Edwardsiella ictaluri; Ichthyophthirius multifiliis; Channel catfish; Mortality; Transmission ID ENTERIC SEPTICEMIA; RAINBOW-TROUT; SUSCEPTIBILITY; EXPOSURE AB There is no information available whether fish naturally coinfected with Ichthyophthirius multifiliis (Ich) and Edwardsiella ictaluri can concurrently transmit both pathogens to naive fish. The objective of this study was to expose naive channel catfish (Ictalurus punctatus) to naturally infected fish that carried Ich and E. ictaluri to provide clinical evidence for transmission of both pathogens. Three tanks of fish were exposed to naturally coinfected fish and two tanks were utilized as mock-infected controls in each of two trials. In trial I, 34 out of 60 fish (56.7%) exposed to two infected fish per tank died at day one. All remaining fish died two days post exposure. Of the dead fish, all showed heavy Ich infection and E. ictaluri was isolated from the kidney of 60% of the dead catfish. In trial II, the cumulative mortality in fish exposed to 2 coinfected fish per tank was less than 20% during days 1-7 post exposure. Most of the fish died from 8 to 14 days post exposure to the coinfected fish. Ninety-six percent of fish were positive for both Ich and E. ictaluri in trial II. The results demonstrated that fish naturally coinfected with Ich and E. ictaluri could concomitantly transmit both pathogens to naive fish. In aquaculture management, precaution is needed to thoroughly examine fish prior to shipment or purchase to prevent the spread of aquatic animal pathogens. Published by Elsevier B.V. C1 [Xu, De-Hai; Shoemaker, Craig A.; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. [Zhang, Qizhong] Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China. RP Xu, DH (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM dehai.xu@ars.usda.gov FU USDA/ARS CRIS [6420-32000-024-00D] FX The authors are grateful to Dr. Thomas Welker, Hagerman Fish Culture Experiment Station, Hagerman, Idaho and Dr. Dunhua Zhang, USDA, ARS, Aquatic Animal Health Research Unit, Auburn, Alabama for critical reviews of the manuscript. We thank Jana Mladek and Paige Mumma for their technical support in the laboratory. This study was supported by the USDA/ARS CRIS project #6420-32000-024-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 16 TC 1 Z9 1 U1 1 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD FEB 15 PY 2013 VL 376 BP 133 EP 136 DI 10.1016/j.aquaculture.2012.11.021 PG 4 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 071EK UT WOS:000313569100019 ER PT J AU Rastogi, G Gurram, RN Bhalla, A Gonzalez, R Bischoff, KM Hughes, SR Kumar, S Sani, RK AF Rastogi, Gurdeep Gurram, Raghu N. Bhalla, Aditya Gonzalez, Ramon Bischoff, Kenneth M. Hughes, Stephen R. Kumar, Sudhir Sani, Rajesh K. TI Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE bioenergy; bioethanol; biofuels; fermentation; gold mine; 1,3-propanediol ID FUEL ETHANOL-PRODUCTION; CLOSTRIDIUM-BUTYRICUM VPI-3266; ESCHERICHIA-COLI KO11; SEA HYDROTHERMAL VENT; KLEBSIELLA-PNEUMONIAE; CRUDE GLYCEROL; SP-NOV.; BIODIESEL PRODUCTION; GEN. NOV.; 1,3-PROPANEDIOL AB Eight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.205-0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585g of acetic acid/g of xylose at the end of fermentation. At pH 7 glycerol-fermenting isolates produced PDO (0.323-0.458 g of PDO/g of glycerol) and ethanol (0.284-0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO. C1 [Rastogi, Gurdeep; Gurram, Raghu N.; Bhalla, Aditya; Kumar, Sudhir; Sani, Rajesh K.] South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, Rapid City, SD 57701 USA. [Gonzalez, Ramon] Rice Univ, Dept Chem & Biomol Engn, Houston, TX USA. [Gonzalez, Ramon] Rice Univ, Dept Bioengn, Houston, TX USA. [Bischoff, Kenneth M.; Hughes, Stephen R.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Sani, RK (reprint author), South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, 501 East St,Joseph St, Rapid City, SD 57701 USA. EM rajesh.sani@sdsmt.edu RI Gonzalez, Ramon/B-1961-2010; BHALLA, ADITYA/Q-2792-2015 OI BHALLA, ADITYA/0000-0003-3462-9600 FU Nelson Research Award funds; South Dakota Board of Regents Competitive Research Grant [SDBOR/SDSMT 2010-09-05]; National Science Foundation - Industry/University Cooperative Research Center (NSF-I/UCRC) [441087]; office of Vice President for Research and Department of Chemical and Biological Engineering at the South Dakota School of Mines and Technology FX This research was funded by the Nelson Research Award funds and South Dakota Board of Regents Competitive Research Grant (Award No. SDBOR/SDSMT 2010-09-05). Aditya Bhalla and Rajesh Sani gratefully acknowledge the financial support provided by National Science Foundation - Industry/University Cooperative Research Center (NSF-I/UCRC, Grant #441087). The support from the office of Vice President for Research and Department of Chemical and Biological Engineering at the South Dakota School of Mines and Technology is also acknowledged. Sudhir Kumar is thankful to Jaypee University of information Technology, Solan, HP, India for granting a sabbatical leave for doing research work at the South Dakota School of Mines and Technology, SD, USA. NR 47 TC 4 Z9 4 U1 0 U2 16 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 15 PY 2013 VL 4 AR 18 DI 10.3389/fmicb.2013.00018 PG 8 WC Microbiology SC Microbiology GA AA3AE UT WOS:000330964500001 PM 23919089 ER PT J AU Hollingsworth, TN Johnstone, JF Bernhardt, EL Chapin, FS AF Hollingsworth, Teresa N. Johnstone, Jill F. Bernhardt, Emily L. Chapin, F. Stuart, III TI Fire Severity Filters Regeneration Traits to Shape Community Assembly in Alaska's Boreal Forest SO PLOS ONE LA English DT Article ID PLANT FUNCTIONAL TRAITS; VEGETATION RESPONSE; SPECIES COMPOSITION; TREE RECRUITMENT; INTERIOR ALASKA; SCALE; ECOSYSTEMS; CLIMATE; BIOMASS; REGIME AB Disturbance can both initiate and shape patterns of secondary succession by affecting processes of community assembly. Thus, understanding assembly rules is a key element of predicting ecological responses to changing disturbance regimes. We measured the composition and trait characteristics of plant communities early after widespread wildfires in Alaska to assess how variations in disturbance characteristics influenced the relative success of different plant regeneration strategies. We compared patterns of post-fire community composition and abundance of regeneration traits across a range of fire severities within a single pre-fire forest type-black spruce forests of Interior Alaska. Patterns of community composition, as captured by multivariate ordination with nonmetric multidimensional scaling, were primarily related to gradients in fire severity (biomass combustion and residual vegetation) and secondarily to gradients in soil pH and regional climate. This pattern was apparent in both the full dataset (n = 87 sites) and for a reduced subset of sites (n = 49) that minimized the correlation between site moisture and fire severity. Changes in community composition across the fire-severity gradient in Alaska were strongly correlated to variations in plant regeneration strategy and rooting depth. The tight coupling of fire severity with regeneration traits and vegetation composition after fire supports the hypothesis that disturbance characteristics influence patterns of community assembly by affecting the relative success of different regeneration strategies. This study further demonstrated that variations in disturbance characteristics can dominate over environmental constraints in determining early patterns of community assembly. By affecting the success of regeneration traits, changes in fire regime directly shape the outcomes of community assembly, and thus may override the effects of slower environmental change on boreal forest composition. C1 [Hollingsworth, Teresa N.] US Forest Serv, USDA, Pacific NW Res Stn, Fairbanks, AK USA. [Johnstone, Jill F.] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 0W0, Canada. [Bernhardt, Emily L.] Univ Alaska Fairbanks, Dept Biol & Wildlife, Fairbanks, AK USA. [Chapin, F. Stuart, III] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK USA. RP Hollingsworth, TN (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Fairbanks, AK USA. EM thollingsworth@fs.fed.us RI Johnstone, Jill/C-9204-2009; OI Johnstone, Jill/0000-0001-6131-9339; Chapin III, F Stuart/0000-0002-2558-9910 FU U. S. Joint Fire Science Program [05-1-2-06]; NSERC FX This research was supported by funding from the U. S. Joint Fire Science Program (project 05-1-2-06), a NSERC Discovery grant to JFJ, and in-kind support from the Bonanza Creek Long-Term Ecological Research site and U. S. Forest Service PNW Research Station. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 23 Z9 23 U1 8 U2 95 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 13 PY 2013 VL 8 IS 2 AR e56033 DI 10.1371/journal.pone.0056033 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104DM UT WOS:000315970300095 PM 23418503 ER PT J AU Munch, EM Harris, RA Mohammad, M Benham, AL Pejerrey, SM Showalter, L Hu, M Shope, CD Maningat, PD Gunaratne, PH Haymond, M Aagaard, K AF Munch, Erika M. Harris, R. Alan Mohammad, Mahmoud Benham, Ashley L. Pejerrey, Sasha M. Showalter, Lori Hu, Min Shope, Cynthia D. Maningat, Patricia D. Gunaratne, Preethi H. Haymond, Morey Aagaard, Kjersti TI Transcriptome Profiling of microRNA by Next-Gen Deep Sequencing Reveals Known and Novel miRNA Species in the Lipid Fraction of Human Breast Milk SO PLOS ONE LA English DT Article ID CIRCULATING MICRORNAS; IMMUNE-SYSTEM; EXPRESSION; LACTATION; EVOLUTION; MACRONUTRIENT; DETERMINANTS; BIOMARKERS; EPIGENOME; EXOSOMES AB While breast milk has unique health advantages for infants, the mechanisms by which it regulates the physiology of newborns are incompletely understood. miRNAs have been described as functioning transcellularly, and have been previously isolated in cell-free and exosomal form from bodily liquids (serum, saliva, urine) and tissues, including mammary tissue. We hypothesized that breast milk in general, and milk fat globules in particular, contain significant numbers of known and limited novel miRNA species detectable with massively parallel sequencing. Extracted RNA from lactating mothers before and following short-term treatment with recombinant human growth hormone (rhGH) was smRNA-enriched. smRNA-Seq was performed to generate 124,110,646 36-nt reads. Of these, 31,102,927 (25%) exactly matched known human miRNAs; with relaxing of stringency, 74,716,151 (60%) matched known miRNAs including 308 of the 1018 (29%) mature miRNAs (miRBase 16.0). These miRNAs are predicted to target 9074 genes; the 10 most abundant of these predicted to target 2691 genes with enrichment for transcriptional regulation of metabolic and immune responses. We identified 21 putative novel miRNAs, of which 12 were confirmed in a large validation set that included cohorts of lactating women consuming enriched diets. Of particular interest, we observed that expression of several novel miRNAs were altered by the perturbed maternal diet, notably following a high-fat intake (p<0.05). Our findings suggest that known and novel miRNAs are enriched in breast milk fat globules, and expression of several novel miRNA species is regulated by maternal diet. Based on robust pathway mapping, our data supports the notion that these maternally secreted miRNAs (stable in the milk fat globules) play a regulatory role in the infant and account in part for the health benefits of breast milk. We further speculate that regulation of these miRNA by a high fat maternal diet enables modulation of fetal metabolism to accommodate significant dietary challenges. C1 [Munch, Erika M.; Harris, R. Alan; Showalter, Lori; Hu, Min; Shope, Cynthia D.; Aagaard, Kjersti] Baylor Coll Med, Dept Obstet & Gynecol, Div Maternal Fetal Med, Houston, TX 77030 USA. [Harris, R. Alan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Mohammad, Mahmoud; Maningat, Patricia D.; Haymond, Morey] Texas Childrens Hosp, Baylor Coll Med, Div Pediat Endocrinol & Metab, Dept Pediat,Childrens Nutr Res Ctr,USDA,ARS, Houston, TX 77030 USA. [Benham, Ashley L.; Gunaratne, Preethi H.] Univ Houston, Dept Biol & Biochem, Houston, TX USA. [Pejerrey, Sasha M.; Aagaard, Kjersti] Baylor Coll Med, Dept Mol & Cell Biol, Houston, TX 77030 USA. [Gunaratne, Preethi H.] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA. RP Aagaard, K (reprint author), Baylor Coll Med, Dept Obstet & Gynecol, Div Maternal Fetal Med, Houston, TX 77030 USA. EM aagaardt@bcm.edu OI Mohammad, Mahmoud/0000-0002-6535-5529 FU National Institutes of Health (NIH) Director New Innovator Award [DP2120OD001500-01]; National Institute of Child Health and Human Development (NICHD)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R01DK080558-01]; NIH Common Fund Human Microbiome-HMP [NIHU54HG004969]; Burroughs Welcome Fund (Burroughs Welcome Fund Preterm Birth Initiative) FX This work was funded by the National Institutes of Health (NIH) Director New Innovator Award (DP2120OD001500-01 KA with funded support to RH), National Institute of Child Health and Human Development (NICHD)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) # R01DK080558-01 (KA), the NIH Common Fund Human Microbiome-HMP (KA (NIHU54HG004969)), and the Burroughs Welcome Fund (Burroughs Welcome Fund Preterm Birth Initiative (KA)). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 46 Z9 47 U1 3 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 13 PY 2013 VL 8 IS 2 AR e50564 DI 10.1371/journal.pone.0050564 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104DM UT WOS:000315970300005 PM 23418415 ER PT J AU Wang, CM Zhang, YY Wu, B Liu, SL Xu, P Lu, YM Luo, J Nolte, DL Deliberto, TJ Duan, MX Zhang, H He, HX AF Wang, Chengmin Zhang, Yanyu Wu, Bin Liu, Shelan Xu, Ping Lu, Yanmin Luo, Jing Nolte, Dale Louis Deliberto, Thomas Jude Duan, Mingxing Zhang, Hong He, Hongxuan TI Evolutionary Characterization of the Pandemic H1N1/2009 Influenza Virus in Humans Based on Non-Structural Genes SO PLOS ONE LA English DT Article ID AMINO-ACID SITES; H1N1 INFLUENZA; REPLICATION; SELECTION; PROTEIN; NUCLEOPROTEIN AB The 2009 influenza pandemic had a tremendous social and economic impact. To study the genetic diversity and evolution of the 2009 H1N1 virus, a mutation network for the non-structural (NS) gene of the virus was constructed. Strains of the 2009 H1N1 pandemic influenza A virus could be divided into two categories based on the V123I mutation in the NS1 gene: G1 (characterized as 123 Val) and G2 (characterized as 123 Ile). Sequence homology analysis indicated that one type of NS sequence, primarily isolated from Mexico, was likely the original type in this pandemic. The two genotypes of the virus presented distinctive clustering features in their geographic distributions. These results provide additional insight into the genetics and evolution of human pandemic influenza H1N1. C1 [Wang, Chengmin; Wu, Bin; Xu, Ping; Lu, Yanmin; Luo, Jing; Zhang, Hong; He, Hongxuan] Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, Natl Res Ctr Wildlife Born Dis, Beijing, Peoples R China. [Nolte, Dale Louis; Deliberto, Thomas Jude] Wildlife Serv, Natl Wildlife Res Ctr, USDA, APHIS, Ft Collins, CO USA. [Duan, Mingxing] Tsinghua Univ, Sch Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China. [Liu, Shelan] Zhejiang Ctr Dis Control & Prevent, Dept Infect Dis, Hangzhou, Zhejiang, Peoples R China. [Zhang, Yanyu] Acad Mil Med Sci, Beijing Inst Transfus Med, Beijing, Peoples R China. RP He, HX (reprint author), Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, Natl Res Ctr Wildlife Born Dis, Beijing, Peoples R China. EM hehx@ioz.ac.cn FU Chinese academy of sciences [KSCX2-EW-J-2]; National Science and Technology Ministry [2009BAI83B01]; National Natural Sciences Foundation of China [31101806]; USDA/APHIS/WS-IOZ CAS joint project [0760621234] FX This study was supported by the knowledge innovation program of the Chinese academy of sciences (KSCX2-EW-J-2), the National Science and Technology Ministry (2009BAI83B01), National Natural Sciences Foundation of China (31101806) and USDA/APHIS/WS-IOZ CAS joint project (0760621234). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 24 TC 2 Z9 2 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 13 PY 2013 VL 8 IS 2 AR e56201 DI 10.1371/journal.pone.0056201 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104DM UT WOS:000315970300127 PM 23418535 ER PT J AU Xia, YS Li, RH Ning, ZX Bai, GH Siddique, KHM Yan, GJ Baum, M Varshney, RK Guo, PG AF Xia, Yanshi Li, Ronghua Ning, Zhengxiang Bai, Guihua Siddique, Kadambot H. M. Yan, Guijun Baum, Michael Varshney, Rajeev K. Guo, Peiguo TI Single Nucleotide Polymorphisms in HSP17.8 and Their Association with Agronomic Traits in Barley SO PLOS ONE LA English DT Article ID HEAT-SHOCK PROTEINS; HORDEUM-VULGARE L.; MOLECULAR DIVERSITY; ABIOTIC STRESSES; WILD BARLEY; POPULATION-STRUCTURE; QUANTITATIVE TRAITS; DROUGHT TOLERANCE; DNA POLYMORPHISM; GENES AB Small heat shock protein 17.8 (HSP17.8) is produced abundantly in plant cells under heat and other stress conditions and may play an important role in plant tolerance to stress environments. However, HSP17.8 may be differentially expressed in different accessions of a crop species exposed to identical stress conditions. The ability of different genotypes to adapt to various stress conditions resides in their genetic diversity. Allelic variations are the most common forms of genetic variation in natural populations. In this study, single nucleotide polymorphisms (SNPs) of the HSP17.8 gene were investigated across 210 barley accessions collected from 30 countries using EcoTILLING technology. Eleven SNPs including 10 from the coding region of HSP17.8 were detected, which form nine distinguishable haplotypes in the barley collection. Among the 10 SNPs in the coding region, six are missense mutations and four are synonymous nucleotide changes. Five of the six missense changes are predicted to be deleterious to HSP17.8 function. The accessions from Middle East Asia showed the higher nucleotide diversity of HSP17.8 than those from other regions and wild barley (H. spontaneum) accessions exhibited greater diversity than the cultivated barley (H. vulgare) accessions. Four SNPs in HSP17.8 were found associated with at least one of the agronomic traits evaluated except for spike length, namely number of grains per spike, thousand kernel weight, plant height, flag leaf area and leaf color. The association between SNP and these agronomic traits may provide new insight for study of the gene's potential contribution to drought tolerance of barley. C1 [Xia, Yanshi; Li, Ronghua; Guo, Peiguo] Guangzhou Univ, Coll Life Sci, Int Crop Res Ctr Stress Resistance, Guangzhou, Guangdong, Peoples R China. [Xia, Yanshi; Ning, Zhengxiang] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou, Guangdong, Peoples R China. [Bai, Guihua] ARS, Hard Winter Wheat Genet Res Unit, USDA, Manhattan, KS USA. [Siddique, Kadambot H. M.; Yan, Guijun] Univ Western Australia, Inst Agr, Perth, WA 6009, Australia. [Baum, Michael] Int Ctr Agr Res Dry Areas, Aleppo, Syria. [Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Greater Hyderab, India. RP Guo, PG (reprint author), Guangzhou Univ, Coll Life Sci, Int Crop Res Ctr Stress Resistance, Guangzhou, Guangdong, Peoples R China. EM guopg@yahoo.com RI Siddique, Kadambot /B-3462-2011; Varshney, Rajeev/C-5295-2014; Yan, Guijun/A-9809-2011 OI Varshney, Rajeev/0000-0002-4562-9131; Yan, Guijun/0000-0001-9628-1211 FU National Natural Science Foundation of China [30871526]; Guangdong Province the Technology Plan in China [2008B050300003] FX This work was funded by the National Natural Science Foundation of China (30871526) (http://www.nsfc.gov.cn) and Guangdong Province the Technology Plan in China (2008B050300003) (http://www.gdstc.gov.cn). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 5 Z9 7 U1 2 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 13 PY 2013 VL 8 IS 2 AR e56816 DI 10.1371/journal.pone.0056816 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104DM UT WOS:000315970300196 PM 23418603 ER PT J AU Kasarda, DD AF Kasarda, Donald D. TI Can an Increase in Celiac Disease Be Attributed to an Increase in the Gluten Content of Wheat as a Consequence of Wheat Breeding? SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE gluten; celiac disease; breeding; wheat protein content ID QUALITY; TRENDS AB In response to the suggestion that an increase in the incidence of celiac disease might be attributable to an increase in the gluten content of wheat resulting from wheat breeding, a survey of data from the 20th and 21st centuries for the United States was carried out. The results do not support the likelihood that wheat breeding has increased the protein content (proportional to gluten content) of wheat in the United States. Possible roles for changes in the per capita consumption of wheat flour and the use of vital gluten as a food additive are discussed. C1 ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Kasarda, DD (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. NR 25 TC 30 Z9 31 U1 3 U2 50 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 13 PY 2013 VL 61 IS 6 BP 1155 EP 1159 DI 10.1021/jf305122s PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 091WF UT WOS:000315079800001 PM 23311690 ER PT J AU Schrader, KK Avolio, F Andolfi, A Cimmino, A Evidente, A AF Schrader, Kevin K. Avolio, Fabiana Andolfi, Anna Cimmino, Alessio Evidente, Antonio TI Ungeremine and Its Hemisynthesized Analogues as Bactericides against Flavobacterium columnare SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE alkaloids; Amaryllidaceae; bactericides; channel catfish; Flavobacterium columnare; Pancratium maritimum; ungeremine ID CHANNEL CATFISH; ALKALOIDS; LYCORINE; AMARYLLIDACEAE; MORTALITY AB The Gram-negative bacterium Flavobacterium columnare is the cause of columnaris disease, which can occur in channel catfish (Ictalurus punctatus). In a previous study, the betaine-type alkaloid ungeremine, 1, obtained from Pancratium maritimum L. was found to have strong antibacterial activity against F. columnare. In this study, analogues of 1 were evaluated using a rapid bioassay for activity against F. columnare to determine if the analogues might provide greater antibacterial activity and to determine structure-activity relationships of the test compounds. Several ungeremine analogues were prepared by hydrochlorination of the alkaloid and by selenium dioxide oxidation of both lycorine, 7, and pseudolycorine, 8, which yielded the isomer of ungeremine, 3, and zefbetaine, 4, respectively. The treatment of lycorine with phosphorus oxychloride allowed the synthesis of an anhydrolycorine lactam, 5, showing, with respect to 1, the deoxygenation and oxygenation of C-2 and C-7 of the C and B rings, respectively. The results of the structure-activity relationship studies showed that the aromatization of the C ring and the oxidation to an azomethine group of C-7 of the B ring are structural features important for antibacterial activity. In addition, the position of the oxygenation of the C ring as well as the presence of the 1,3-dioxole ring joined to the A ring of the pyrrolo[de]phenanthridine skeleton also plays a significant role in imparting antibacterial activity. On the basis of 24-h 50% inhibition concentration (IC50) results, ungeremine hydrochloride, 2, was similar in toxicity to 1, whereas 5 had the lowest activity. Analogue 2 is soluble in water, which may provide the benefit for use as an effective feed additive or therapeutant compared to ungeremine. C1 [Schrader, Kevin K.] Univ Mississippi, ARS, USDA, Nat Prod Utilizat Res Unit,Thad Cochran Natl Ctr, University, MS 38677 USA. [Avolio, Fabiana; Andolfi, Anna; Cimmino, Alessio; Evidente, Antonio] Univ Naples Federico II, Complesso Univ Monte St Angelo, Dipartimento Sci Chim, I-80126 Naples, Italy. [Evidente, Antonio] CNR, Ist Chim Biomol, Pozzuoli, Italy. RP Schrader, KK (reprint author), Univ Mississippi, ARS, USDA, Nat Prod Utilizat Res Unit,Thad Cochran Natl Ctr, POB 8048, University, MS 38677 USA. EM kevin.schrader@ars.usda.gov RI Evidente, Antonio/N-9357-2013; Cimmino, Alessio/I-2121-2012 OI Evidente, Antonio/0000-0001-9110-1656; Cimmino, Alessio/0000-0002-1551-4237 FU Italian Ministry of University and Research FX This work was supported in part by a grant from the Italian Ministry of University and Research. NR 18 TC 9 Z9 9 U1 0 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 13 PY 2013 VL 61 IS 6 BP 1179 EP 1183 DI 10.1021/jf304586j PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 091WF UT WOS:000315079800005 PM 23331165 ER PT J AU Leisso, R Buchanan, D Lee, J Mattheis, J Rudell, D AF Leisso, Rachel Buchanan, David Lee, Jinwook Mattheis, James Rudell, David TI Cell Wall, Cell Membrane, and Volatile Metabolism Are Altered by Antioxidant Treatment, Temperature Shifts, and Peel Necrosis during Apple Fruit Storage SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE superficial scald; antioxidant; diphenylamine; volatile aroma esters; shelf life; ripening ID GRANNY-SMITH APPLES; SUPERFICIAL SCALD; ALPHA-FARNESENE; STORED APPLES; HIGHER-PLANTS; CHLOROPHYLL DEGRADATION; FUNCTIONAL DISORDER; MASS-SPECTROMETRY; BIOSYNTHESIS; DIPHENYLAMINE AB The transition from cold storage to ambient temperature alters apple quality through accelerated softening, flavor and color changes, and development of physiological peel disorders, such as superficial scald, in susceptible cultivars. To reveal global metabolism associated with this transition, the 'Granny Smith' peel metabolome was evaluated during storage of 6 months and shelf life periods. Treatment with the antioxidant diphenylamine (DPA) reduced scald, creating a metabolic contrast with untreated fruit, which developed superficial scald. Superficial scald symptoms developed on control fruit after 120 days of storage, and symptoms progressed following transition to ambient-temperature shelf life. The metabolic profile of control and DPA-treated fruit was divergent after 30 days of cold storage due to differing levels of a-farnesene oxidation products, methyl esters, phytosterols, and other compounds potentially associated with chloroplast integrity and oxidative stress response. Hierarchical cluster analysis revealed coregulation within the volatile synthesis pathway including control of the availability of methyl, propyl, ethyl, acetyl, and butyl alcohol and/or acid moieties for ester biosynthesis. Overall, the application of metabolomics techniques lends new insight into physiological processes leading to cell death and ripening processes that affect fruit flavor, appearance, and overall quality. C1 [Leisso, Rachel; Buchanan, David; Lee, Jinwook; Mattheis, James; Rudell, David] ARS, Tree Fruit Res Lab, USDA, Wenatchee, WA 98801 USA. RP Rudell, D (reprint author), ARS, Tree Fruit Res Lab, USDA, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM David.Rudell@ars.usda.gov RI sebastianovitsch, stepan/G-8507-2013 NR 53 TC 9 Z9 9 U1 2 U2 68 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 13 PY 2013 VL 61 IS 6 BP 1373 EP 1387 DI 10.1021/jf3046208 PG 15 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 091WF UT WOS:000315079800029 PM 23311914 ER PT J AU Mamidala, P Wijeratne, AJ Wijeratne, S Poland, T Qazi, SS Doucet, D Cusson, M Beliveau, C Mittapalli, O AF Mamidala, Praveen Wijeratne, Asela J. Wijeratne, Saranga Poland, Therese Qazi, Sohail S. Doucet, Daniel Cusson, Michel Beliveau, Catherine Mittapalli, Omprakash TI Identification of Odor-Processing Genes in the Emerald Ash Borer, Agrilus planipennis SO PLOS ONE LA English DT Article ID PHEROMONE-DEGRADING ENZYME; FAIRMAIRE COLEOPTERA BUPRESTIDAE; IONOTROPIC GLUTAMATE RECEPTORS; PROTEIN-PROTEIN INTERACTIONS; MOTH HELIOTHIS-VIRESCENS; SEX-PHEROMONE; DROSOPHILA-MELANOGASTER; MANDUCA-SEXTA; ANTHERAEA-POLYPHEMUS; OLFACTORY RECEPTORS AB Background: Insects rely on olfaction to locate food, mates, and suitable oviposition sites for successful completion of their life cycle. Agrilus planipennis Fairmaire (emerald ash borer) is a serious invasive insect pest that has killed tens of millions of North American ash (Fraxinus spp) trees and threatens the very existence of the genus Fraxinus. Adult A. planipennis are attracted to host volatiles and conspecifics; however, to date no molecular knowledge exists on olfaction in A. planipennis. Hence, we undertook an antennae-specific transcriptomic study to identify the repertoire of odor processing genes involved in A. planipennis olfaction. Methodology and Principal Findings: We acquired 139,085 Roche/454 GS FLX transcriptomic reads that were assembled into 30,615 high quality expressed sequence tags (ESTs), including 3,249 isotigs and 27,366 non-isotigs (contigs and singletons). Intriguingly, the majority of the A. planipennis antennal transcripts (59.72%) did not show similarity with sequences deposited in the non-redundant database of GenBank, potentially representing novel genes. Functional annotation and KEGG analysis revealed pathways associated with signaling and detoxification. Several odor processing genes (9 odorant binding proteins, 2 odorant receptors, 1 sensory neuron membrane protein and 134 odorant/xenobiotic degradation enzymes, including cytochrome P450s, glutathione-S-transferases; esterases, etc.) putatively involved in olfaction processes were identified. Quantitative PCR of candidate genes in male and female A. planipennis in different developmental stages revealed developmental- and sex-biased expression patterns. Conclusions and Significance: The antennal ESTs derived from A. planipennis constitute a rich molecular resource for the identification of genes potentially involved in the olfaction process of A. planipennis. These findings should help in understanding the processing of antennally-active compounds (e.g. 7-epi-sesquithujene) previously identified in this serious invasive pest. C1 [Mamidala, Praveen; Mittapalli, Omprakash] Ohio State Univ, Ohio Agr & Res Dev Ctr, Dept Entomol, Wooster, OH USA. [Wijeratne, Asela J.; Wijeratne, Saranga] Ohio State Univ, Ohio Agr & Res Dev Ctr, Dept Mol & Cellular Imaging Ctr, Wooster, OH USA. [Poland, Therese] Michigan State Univ, USDA, US Forest Serv, No Res Stn, E Lansing, MI 48824 USA. [Qazi, Sohail S.; Doucet, Daniel] Nat Resources Canada, Sault Ste Marie, ON, Canada. [Cusson, Michel; Beliveau, Catherine] Nat Resources Canada, Quebec City, PQ, Canada. RP Mittapalli, O (reprint author), Ohio State Univ, Ohio Agr & Res Dev Ctr, Dept Entomol, Wooster, OH USA. EM mittapalli.1@osu.edu RI Wijeratne, Saranga/I-5341-2016; OI Wijeratne, Saranga/0000-0002-9782-4406; Qazi, Sohail/0000-0003-1317-6657 FU USDA APHIS/USDA Forest Service Accelerated Emerald Ash Borer Research Program [GRT00011769/60016270]; State and Federal funds appropriated to the Ohio Agricultural Research and Development Center; The Ohio State University; Genomic Research and Development Initiative grant from Natural Resources Canada FX This research was supported by a grant from the USDA APHIS (GRT00011769/60016270)/USDA Forest Service Accelerated Emerald Ash Borer Research Program, by State and Federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University and by a Genomic Research and Development Initiative grant from Natural Resources Canada. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 110 TC 14 Z9 15 U1 0 U2 45 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 12 PY 2013 VL 8 IS 2 AR e56555 DI 10.1371/journal.pone.0056555 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 104BU UT WOS:000315965100056 PM 23424668 ER PT J AU Fang, SQ Clark, RT Zheng, Y Iyer-Pascuzzi, AS Weitz, JS Kochian, LV Edelsbrunner, H Liao, H Benfey, PN AF Fang, Suqin Clark, Randy T. Zheng, Ying Iyer-Pascuzzi, Anjali S. Weitz, Joshua S. Kochian, Leon V. Edelsbrunner, Herbert Liao, Hong Benfey, Philip N. TI Genotypic recognition and spatial responses by rice roots SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE 3D reconstruction; imaging; kin recognition ID BELOW-GROUND COMPETITION; KIN RECOGNITION; PHENOTYPIC PLASTICITY; SYSTEM ARCHITECTURE; SELF-RECOGNITION; PLANT; COMMUNICATION; PHYTOCHROME; NEIGHBORS; NUTRIENTS AB Root system growth and development is highly plastic and is influenced by the surrounding environment. Roots frequently grow in heterogeneous environments that include interactions from neighboring plants and physical impediments in the rhizosphere. To investigate how planting density and physical objects affect root system growth, we grew rice in a transparent gel system in close proximity with another plant or a physical object. Root systems were imaged and reconstructed in three dimensions. Root-root interaction strength was calculated using quantitative metrics that characterize the extent to which the reconstructed root systems overlap each other. Surprisingly, we found the overlap of root systems of the same genotype was significantly higher than that of root systems of different genotypes. Root systems of the same genotype tended to grow toward each other but those of different genotypes appeared to avoid each other. Shoot separation experiments excluded the possibility of aerial interactions, suggesting root communication. Staggered plantings indicated that interactions likely occur at root tips in close proximity. Recognition of obstacles also occurred through root tips, but through physical contact in a size-dependent manner. These results indicate that root systems use two different forms of communication to recognize objects and alter root architecture: root-root recognition, possibly mediated through root exudates, and root-object recognition mediated by physical contact at the root tips. This finding suggests that root tips act as local sensors that integrate rhizosphere information into global root architectural changes. C1 [Fang, Suqin; Iyer-Pascuzzi, Anjali S.; Benfey, Philip N.] Duke Univ, Dept Biol, Duke Ctr Syst Biol, Durham, NC 27708 USA. [Fang, Suqin; Liao, Hong] South China Agr Univ, Root Biol Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China. [Clark, Randy T.] Cornell Univ, USDA ARS, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. [Clark, Randy T.; Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Zheng, Ying; Edelsbrunner, Herbert] Duke Univ, Dept Comp Sci, Durham, NC 27708 USA. [Weitz, Joshua S.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Weitz, Joshua S.] Georgia Inst Technol, Sch Phys, Atlanta, GA 30332 USA. [Edelsbrunner, Herbert] IST Austria, A-3400 Klosterneuburg, Austria. RP Liao, H (reprint author), South China Agr Univ, Root Biol Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China. EM hliao@scau.edu.cn; philip.benfey@duke.edu OI Kochian, Leon/0000-0003-3416-089X FU National Science Foundation [DBI-0820624]; National Natural Science Foundation of China [31025022]; Burroughs Wellcome Fund FX We thank Prof. Susan McCouch for providing the seeds used in these studies; Olivia Haragutchi and Medhavinee Mijar for technical help; and members of the P.N.B. laboratory for comments on the manuscript. This work was supported by National Science Foundation Grant (Genome-Wide Analysis of Root Traits) DBI-0820624 (to P.N.B. and J.S.W.) and National Natural Science Foundation of China Grant 31025022 (to H.L.). J.S.W. holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. NR 40 TC 39 Z9 48 U1 8 U2 157 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 12 PY 2013 VL 110 IS 7 BP 2670 EP 2675 DI 10.1073/pnas.1222821110/-/DCSupplemental PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 101ZL UT WOS:000315812800060 PM 23362379 ER PT J AU Morin, E Kohler, A Baker, AR Foulongne-Oriol, M Lombard, V Nagy, LG Ohm, RA Patyshakuliyeva, A Brun, A Aerts, AL Bailey, AM Billette, C Coutinho, PM Deakin, G Doddapaneni, H Floudas, D Grimwood, J Hilden, K Kues, U LaButti, KM Lapidus, A Lindquist, EA Lucas, SM Murat, C Riley, RW Salamov, AA Schmutz, J Subramanian, V Wosten, HAB Xu, JP Eastwood, DC Foster, GD Sonnenberg, ASM Cullen, D de Vries, RP Lundell, T Hibbett, DS Henrissat, B Burton, KS Kerrigan, RW Challen, MP Grigoriev, IV Martin, F AF Morin, Emmanuelle Kohler, Annegret Baker, Adam R. Foulongne-Oriol, Marie Lombard, Vincent Nagy, Laszlo G. Ohm, Robin A. Patyshakuliyeva, Aleksandrina Brun, Annick Aerts, Andrea L. Bailey, Andrew M. Billette, Christophe Coutinho, Pedro M. Deakin, Greg Doddapaneni, Harshavardhan Floudas, Dimitrios Grimwood, Jane Hilden, Kristiina Kuees, Ursula LaButti, Kurt M. Lapidus, Alla Lindquist, Erika A. Lucas, Susan M. Murat, Claude Riley, Robert W. Salamov, Asaf A. Schmutz, Jeremy Subramanian, Venkataramanan Wosten, Han A. B. Xu, Jianping Eastwood, Daniel C. Foster, Gary D. Sonnenberg, Anton S. M. Cullen, Dan de Vries, Ronald P. Lundell, Taina Hibbett, David S. Henrissat, Bernard Burton, Kerry S. Kerrigan, Richard W. Challen, Michael P. Grigoriev, Igor V. Martin, Francis TI Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche (vol 109, pg 17501, 2012) SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Correction C1 [Morin, Emmanuelle; Kohler, Annegret; Brun, Annick; Murat, Claude; Martin, Francis] Univ Henri Poincare, UMR 1136, Inst Natl Rech Agron, F-54280 Champenoux, France. [Baker, Adam R.] Univ Warwick, Warwick CV35 9EF, England. [Foulongne-Oriol, Marie; Billette, Christophe] Inst Natl Rech Agron, Mycol & Securite Aliments UR1264, F-33883 Villenave Dornon, France. [Lombard, Vincent; Coutinho, Pedro M.; Henrissat, Bernard] Aix Marseille Univ, CNRS, UMR 7257, F-13288 Marseille 9, France. [Nagy, Laszlo G.; Floudas, Dimitrios; Hibbett, David S.] Clark Univ, Biol Dept, Worcester, MA 01610 USA. [Ohm, Robin A.; Aerts, Andrea L.; LaButti, Kurt M.; Lapidus, Alla; Lindquist, Erika A.; Lucas, Susan M.; Riley, Robert W.; Salamov, Asaf A.; Grigoriev, Igor V.] US Dept Energy Joint Genome Inst, Walnut Creek, CA 94598 USA. [Ohm, Robin A.; Wosten, Han A. B.] Univ Utrecht, Dept Microbiol, NL-3584 CH Utrecht, Netherlands. [Patyshakuliyeva, Aleksandrina; de Vries, Ronald P.] Royal Netherlands Acad Arts & Sci, Fungal Biodivers Ctr, Cent Bur Schimmelcultures, NL-3584 CT Utrecht, Netherlands. [Bailey, Andrew M.; Foster, Gary D.] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England. [Deakin, Greg; Burton, Kerry S.] East Malling Res, East Malling ME19 6BJ, Kent, England. [Deakin, Greg] Mushroom Res Grp, Crops Environm & Land Use Programme, Dublin 15, Ireland. [Doddapaneni, Harshavardhan] Univ Iowa, Dept Biol, Carver Ctr Genom, Iowa City, IA 52242 USA. [Grimwood, Jane; Schmutz, Jeremy; Lundell, Taina] US Dept Energy Joint Genome Inst, Hudson Alpha Inst, Huntsville, AL 35806 USA. [Hilden, Kristiina] Univ Helsinki, Dept Food & Environm Sci, Div Microbiol, FI-00014 Helsinki, Finland. [Kuees, Ursula] Univ Gottingen, Busgen Inst, Dept Mol Wood Biotechnol & Tech Mycol, D-37077 Gottingen, Germany. [Subramanian, Venkataramanan] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA. [Xu, Jianping] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada. [Eastwood, Daniel C.] Univ Swansea, Coll Sci, Dept Biosci, Swansea SA2 8PP, W Glam, Wales. [Sonnenberg, Anton S. M.] Wageningen Univ, Plant Res Int Mushrooms, NL-6700 AA Wageningen, Netherlands. [Cullen, Dan] Forest Prod Lab, Madison, WI 53726 USA. [Kerrigan, Richard W.] Sylvan Biosci, Kittanning, PA 16201 USA. [Challen, Michael P.] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England. RP Morin, E (reprint author), Univ Henri Poincare, UMR 1136, Inst Natl Rech Agron, F-54280 Champenoux, France. RI Lapidus, Alla/I-4348-2013; Schmutz, Jeremy/N-3173-2013; de Vries, Ronald/F-8125-2011 OI Lapidus, Alla/0000-0003-0427-8731; Schmutz, Jeremy/0000-0001-8062-9172; de Vries, Ronald/0000-0002-4363-1123 NR 1 TC 0 Z9 0 U1 0 U2 15 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 12 PY 2013 VL 110 IS 7 BP 4146 EP 4146 DI 10.1073/pnas.1300201110 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 101ZL UT WOS:000315812800010 ER PT J AU Ambrosio, AB do Nascimento, LC Oliveira, BV Teixeira, PJPL Tiburcio, RA Thomazella, DPT Leme, AFP Carazzolle, MF Vidal, RO Mieczkowski, P Meinhardt, LW Pereira, GAG Cabrera, OG AF Ambrosio, Alinne Batista do Nascimento, Leandro Costa Oliveira, Bruno V. Teixeira, Paulo Jose P. L. Tiburcio, Ricardo A. Toledo Thomazella, Daniela P. Leme, Adriana F. P. Carazzolle, Marcelo F. Vidal, Ramon O. Mieczkowski, Piotr Meinhardt, Lyndel W. Pereira, Goncalo A. G. Cabrera, Odalys G. TI Global analyses of Ceratocystis cacaofunesta mitochondria: from genome to proteome SO BMC GENOMICS LA English DT Article DE Cacao wilt disease; Ceratocystis cacaofunesta; Mitogenomics; Mitochondrial proteome; Fungal virulence ID PLANT-PATHOGENIC FUNGI; RNA-SEQ DATA; ALTERNATIVE OXIDASE; NEUROSPORA-CRASSA; MYCOSPHAERELLA-GRAMINICOLA; MONILIOPHTHORA-PERNICIOSA; SACCHAROMYCES-CEREVISIAE; HOMING ENDONUCLEASES; FUSARIUM-OXYSPORUM; OXIDATIVE STRESS AB Background: The ascomycete fungus Ceratocystis cacaofunesta is the causal agent of wilt disease in cacao, which results in significant economic losses in the affected producing areas. Despite the economic importance of the Ceratocystis complex of species, no genomic data are available for any of its members. Given that mitochondria play important roles in fungal virulence and the susceptibility/resistance of fungi to fungicides, we performed the first functional analysis of this organelle in Ceratocystis using integrated "omics" approaches. Results: The C. cacaofunesta mitochondrial genome (mtDNA) consists of a single, 103,147-bp circular molecule, making this the second largest mtDNA among the Sordariomycetes. Bioinformatics analysis revealed the presence of 15 conserved genes and 37 intronic open reading frames in C. cacaofunesta mtDNA. Here, we predicted the mitochondrial proteome (mtProt) of C. cacaofunesta, which is comprised of 1,124 polypeptides - 52 proteins that are mitochondrially encoded and 1,072 that are nuclearly encoded. Transcriptome analysis revealed 33 probable novel genes. Comparisons among the Gene Ontology results of the predicted mtProt of C. cacaofunesta, Neurospora crassa and Saccharomyces cerevisiae revealed no significant differences. Moreover, C. cacaofunesta mitochondria were isolated, and the mtProt was subjected to mass spectrometric analysis. The experimental proteome validated 27% of the predicted mtProt. Our results confirmed the existence of 110 hypothetical proteins and 7 novel proteins of which 83 and 1, respectively, had putative mitochondrial localization. Conclusions: The present study provides the first partial genomic analysis of a species of the Ceratocystis genus and the first predicted mitochondrial protein inventory of a phytopathogenic fungus. In addition to the known mitochondrial role in pathogenicity, our results demonstrated that the global function analysis of this organelle is similar in pathogenic and non-pathogenic fungi, suggesting that its relevance in the lifestyle of these organisms should be based on a small number of specific proteins and/or with respect to differential gene regulation. In this regard, particular interest should be directed towards mitochondrial proteins with unknown function and the novel protein that might be specific to this species. Further functional characterization of these proteins could enhance our understanding of the role of mitochondria in phytopathogenicity. C1 [Ambrosio, Alinne Batista; do Nascimento, Leandro Costa; Oliveira, Bruno V.; Teixeira, Paulo Jose P. L.; Tiburcio, Ricardo A.; Toledo Thomazella, Daniela P.; Carazzolle, Marcelo F.; Vidal, Ramon O.; Pereira, Goncalo A. G.; Cabrera, Odalys G.] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Genom & Expressao, BR-13083970 Sao Paulo, Brazil. [Leme, Adriana F. P.; Vidal, Ramon O.] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias LNBio, BR-13083970 Sao Paulo, Brazil. [Carazzolle, Marcelo F.] Univ Estadual Campinas, Ctr Nacl Processamento Alto Desempenho, BR-13083970 Sao Paulo, Brazil. [Mieczkowski, Piotr] Univ N Carolina, High Throughput Sequencing Facil, Chapel Hill, NC 27516 USA. [Meinhardt, Lyndel W.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. RP Pereira, GAG (reprint author), Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Genom & Expressao, BR-13083970 Sao Paulo, Brazil. EM goncalo@unicamp.br RI Teixeira, Paulo/C-1546-2013; Pereira, Goncalo /B-7944-2012; de Toledo Thomazella, Daniela/D-2750-2012; Carazzolle, Marcelo Falsarella/C-6503-2012; UNICAMP, CCES - /J-7787-2015; Paes Leme, Adriana/C-9679-2012 OI Paes Leme, Adriana/0000-0001-7959-147X FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2009/50119-9] FX The authors would like to acknowledge to Dr. Tomas Harrington, from the Iowa State University Department of Plant Pathology, who generously donated Ceratocystis cacaofunesta strain C1593. This work was supported by research funding of Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, grant number 2009/50119-9. NR 107 TC 2 Z9 2 U1 0 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 11 PY 2013 VL 14 AR 91 DI 10.1186/1471-2164-14-91 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 109WN UT WOS:000316400900001 PM 23394930 ER PT J AU Hall, CM Busch, JD Scoles, GA Palma-Cagle, KA Ueti, MW Kappmeyer, LS Wagner, DM AF Hall, Carina M. Busch, Joseph D. Scoles, Glen A. Palma-Cagle, Kristina A. Ueti, Massaro W. Kappmeyer, Lowell S. Wagner, David M. TI Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of US introductions SO PARASITES & VECTORS LA English DT Article DE Babesia equi; Theileria equi; Equine piroplasmosis; 18S rRNA gene; Microsatellite; Population genetics ID POLYMERASE-CHAIN-REACTION; TICK BOOPHILUS-MICROPLUS; BABESIA-EQUI; PLASMODIUM; PIROPLASMOSIS; CABALLI; BLOOD AB Background: Theileria equi is a tick-borne apicomplexan hemoparasite that causes equine piroplasmosis. This parasite has a worldwide distribution but the United States was considered to be free of this disease until recently. Methods: We used samples from 37 horses to determine genetic relationships among North American T. equi using the 18S rRNA gene and microsatellites. We developed a DNA fingerprinting panel of 18 microsatellite markers using the first complete genome sequence of T. equi. Results: A maximum parsimony analysis of 18S rRNA sequences grouped the samples into two major clades. The first clade (n = 36) revealed a high degree of nucleotide similarity in U.S. T. equi, with just 0-2 single nucleotide polymorphisms (SNPs) among samples. The remaining sample fell into a second clade that was genetically divergent (48 SNPs) from the other U.S. samples. This sample was collected at the Texas border, but may have originated in Mexico. We genotyped T. equi from the U.S. using microsatellite markers and found a moderate amount of genetic diversity (2-8 alleles per locus). The field samples were mostly from a 2009 Texas outbreak (n = 22) although samples from five other states were also included in this study. Using Weir and Cockerham's F-ST estimator (theta) we found strong population differentiation of the Texas and Georgia subpopulations (theta = 0.414), which was supported by a neighbor-joining tree created with predominant single haplotypes. Single-clone infections were found in 27 of the 37 samples (73%), allowing us to identify 15 unique genotypes. Conclusions: The placement of most T. equi into one monophyletic clade by 18S is suggestive of a limited source of introduction into the U.S. When applied to a broader cross section of worldwide samples, these molecular tools should improve source tracking of T. equi outbreaks and may help prevent the spread of this tick-borne parasite. C1 [Hall, Carina M.; Busch, Joseph D.; Palma-Cagle, Kristina A.; Wagner, David M.] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA. [Scoles, Glen A.; Ueti, Massaro W.; Kappmeyer, Lowell S.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Anim Dis Biotechnol Facil 3003, Pullman, WA 99164 USA. RP Wagner, DM (reprint author), No Arizona Univ, Ctr Microbial Genet & Genom, 1298 S Knoles Dr, Flagstaff, AZ 86011 USA. EM dave.wagner@nau.edu FU CRIS Project [5348-32000-034-00D]; Cowden Endowment at Northern Arizona University FX Sara Davis (USDA) provided outstanding technical assistance. Nancy Kumpula-McWhirter (WSU) provided archived blood samples. Ralph Horn and James Allison (USDA) assisted with animal work. Work at the USDA, ARS, Animal Disease Research Unit was funded by CRIS Project# 5348-32000-034-00D. This work was also supported by the Cowden Endowment at Northern Arizona University. NR 35 TC 7 Z9 7 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 11 PY 2013 VL 6 AR 35 DI 10.1186/1756-3305-6-35 PG 12 WC Parasitology SC Parasitology GA 112UD UT WOS:000316618400001 PM 23399005 ER PT J AU Heaton, MP Kalbfleisch, TS Petrik, DT Simpson, B Kijas, JW Clawson, ML Chitko-McKown, CG Harhay, GP Leymaster, KA AF Heaton, Michael P. Kalbfleisch, Theodore S. Petrik, Dustin T. Simpson, Barry Kijas, James W. Clawson, Michael L. Chitko-McKown, Carol G. Harhay, Gregory P. Leymaster, Kreg A. CA Int Sheep Genomics Consortium TI Genetic Testing for TMEM154 Mutations Associated with Lentivirus Susceptibility in Sheep SO PLOS ONE LA English DT Article ID SMALL RUMINANT LENTIVIRUSES; TRANSMISSION; INFECTION; SEQUENCE AB In sheep, small ruminant lentiviruses cause an incurable, progressive, lymphoproliferative disease that affects millions of animals worldwide. Known as ovine progressive pneumonia virus (OPPV) in the U. S., and Visna/Maedi virus (VMV) elsewhere, these viruses reduce an animal's health, productivity, and lifespan. Genetic variation in the ovine transmembrane protein 154 gene (TMEM154) has been previously associated with OPPV infection in U. S. sheep. Sheep with the ancestral TMEM154 haplotype encoding glutamate (E) at position 35, and either form of an N70I variant, were highly-susceptible compared to sheep homozygous for the K35 missense mutation. Our current overall aim was to characterize TMEM154 in sheep from around the world to develop an efficient genetic test for reduced susceptibility. The average frequency of TMEM154 E35 among 74 breeds was 0.51 and indicated that highly-susceptible alleles were present in most breeds around the world. Analysis of whole genome sequences from an international panel of 75 sheep revealed more than 1,300 previously unreported polymorphisms in a 62 kb region containing TMEM154 and confirmed that the most susceptible haplotypes were distributed worldwide. Novel missense mutations were discovered in the signal peptide (A13V) and the extracellular domains (E31Q, I74F, and I102T) of TMEM154. A matrix-assisted laser desorption/ionization-time-of flight mass spectrometry (MALDI-TOF MS) assay was developed to detect these and six previously reported missense and two deletion mutations in TMEM154. In blinded trials, the call rate for the eight most common coding polymorphisms was 99.4% for 499 sheep tested and 96.0% of the animals were assigned paired TMEM154 haplotypes (i.e., diplotypes). The widespread distribution of highly-susceptible TMEM154 alleles suggests that genetic testing and selection may improve the health and productivity of infected flocks. C1 [Heaton, Michael P.; Clawson, Michael L.; Chitko-McKown, Carol G.; Harhay, Gregory P.; Leymaster, Kreg A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Kalbfleisch, Theodore S.] Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40292 USA. [Kalbfleisch, Theodore S.] Intrepid Bioinformat, Louisville, KY USA. [Petrik, Dustin T.; Simpson, Barry] GeneSeek, Lincoln, NE USA. [Kijas, James W.] CSIRO, Div Anim Food & Hlth Sci, Brisbane, Qld, Australia. RP Heaton, MP (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM mike.heaton@ars.usda.gov; ted.kalbfleisch@louisville.edu RI Coltman, David/A-4453-2012; Dalrymple, Brian/F-8721-2010; Barendse, William/D-8608-2011; Simianer, Henner/A-6574-2015; Kijas, James/A-4656-2011; Slate, Jon/D-2925-2012; OI Gupta, Vidya/0000-0002-6139-1346; Drogemuller, Cord/0000-0001-9773-522X; Coltman, David/0000-0002-1268-2307; Dalrymple, Brian/0000-0003-3891-5233; Barendse, William/0000-0002-5464-0658; Simianer, Henner/0000-0002-7551-3797; Kijas, James/0000-0002-1417-8278; Slate, Jon/0000-0003-3356-5123; Clawson, Michael/0000-0002-3355-5390 FU United States Department of Agriculture, Agricultural Research Service FX Funding for this research was provided by the United States Department of Agriculture, Agricultural Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There were no current external funding sources for this study. NR 18 TC 10 Z9 11 U1 0 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 11 PY 2013 VL 8 IS 2 AR e55490 DI 10.1371/journal.pone.0055490 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 092DU UT WOS:000315100000017 PM 23408992 ER PT J AU Roach, DR Khatibi, PA Bischoff, KM Hughes, SR Donovan, DM AF Roach, Dwayne R. Khatibi, Piyum A. Bischoff, Kenneth M. Hughes, Stephen R. Donovan, David M. TI Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Bacteriophage; Lysin; endolysin; Peptidoglycan; Ethanol; Fermentation; Contamination; Lactic acid bacteria; Lactobacillus; Lactobacilli ID LACTIC-ACID BACTERIA; STAPHYLOCOCCUS-AUREUS; SEQUENCE-ANALYSIS; LYSIS GENES; ENDOLYSIN; IDENTIFICATION; LYSOSTAPHIN; EXPRESSION; CLONING; DOMAIN AB Background: Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial fuel ethanol facilities have found that species of Lactobacillus are predominant. Bacteriophage lytic enzymes are peptidoglycan hydrolases that can degrade the Gram positive cell wall when exposed externally and provide a novel source of antimicrobials that are highly refractory to resistance development. Results: The streptococcal phage LambdaSa2 (lambda Sa2) endolysin demonstrated strong lytic activity towards 17 of 22 strains of lactobacilli, staphylococci or streptococci and maintained an optimal specific activity at pH 5.5 and in the presence of <= 5% ethanol (fermentation conditions) toward L. fermentum. Lactobacillus bacteriophage endolysins LysA, LysA2 and LysgaY showed exolytic activity towards 60% of the lactobacilli tested including four L. fermentum isolates from fuel ethanol fermentations. In turbidity reduction assays LysA was able to reduce optical density >75% for 50% of the sensitive strains and >50% for the remaining strains. LysA2 and LysgaY were only able to decrease cellular turbidity by <50%. Optimal specific activities were achieved for LysA, LysA2, and LysgaY at pH 5.5. The presence of ethanol (<= 5%) did not reduce the lytic activity. Lysins were able to reduce both L. fermentum (BR0315-1) (lambda Sa2 endolysin) and L. reuteri (B-14171) (LysA) contaminants in mock fermentations of corn fiber hydrolysates. Conclusion: Bacteriophage lytic enzymes are strong candidates for application as antimicrobials to control lactic acid bacterial contamination in fuel ethanol fermentations. C1 [Roach, Dwayne R.; Donovan, David M.] USDA, Anim & Nat Resources Inst, Anim Biosci & Biotechnol Lab, Agr Res Serv, Beltsville, MD 20705 USA. [Khatibi, Piyum A.; Bischoff, Kenneth M.; Hughes, Stephen R.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Donovan, DM (reprint author), USDA, Anim & Nat Resources Inst, Anim Biosci & Biotechnol Lab, Agr Res Serv, Beltsville, MD 20705 USA. EM david.donovan@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2010-65504-20420]; US State Dept funds supporting a US-Pakistani; US-Russian collaboration (DMD) FX This work was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010-65504-20420 from the USDA National Institute of Food and Agriculture to KB, SH and DMD and US State Dept funds supporting a US-Pakistani and US-Russian collaboration (DMD). The authors would like to thank Bill Harty and Juli Foster-Frey for excellent technical assistance. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. The USDA is an equal opportunity provider and employer. NR 50 TC 16 Z9 17 U1 0 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD FEB 7 PY 2013 VL 6 AR 20 DI 10.1186/1754-6834-6-20 PG 11 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA 138CI UT WOS:000318485600001 PM 23390890 ER PT J AU Hastie, AR Dong, LL Smith, A Finklestein, J Lam, ET Huo, NX Cao, H Kwok, PY Deal, KR Dvorak, J Luo, MC Gu, Y Xiao, M AF Hastie, Alex R. Dong, Lingli Smith, Alexis Finklestein, Jeff Lam, Ernest T. Huo, Naxin Cao, Han Kwok, Pui-Yan Deal, Karin R. Dvorak, Jan Luo, Ming-Cheng Gu, Yong Xiao, Ming TI Rapid Genome Mapping in Nanochannel Arrays for Highly Complete and Accurate De Novo Sequence Assembly of the Complex Aegilops tauschii Genome SO PLOS ONE LA English DT Article ID WHOLE-GENOME; PHYSICAL MAP; STRUCTURAL VARIATION; RESTRICTION MAPS; WHEAT GENOME; RICE GENOME; GENERATION; VALIDATION; RESOLUTION; CLONE AB Next-generation sequencing (NGS) technologies have enabled high-throughput and low-cost generation of sequence data; however, de novo genome assembly remains a great challenge, particularly for large genomes. NGS short reads are often insufficient to create large contigs that span repeat sequences and to facilitate unambiguous assembly. Plant genomes are notorious for containing high quantities of repetitive elements, which combined with huge genome sizes, makes accurate assembly of these large and complex genomes intractable thus far. Using two-color genome mapping of tiling bacterial artificial chromosomes (BAC) clones on nanochannel arrays, we completed high-confidence assembly of a 2.1-Mb, highly repetitive region in the large and complex genome of Aegilops tauschii, the D-genome donor of hexaploid wheat (Triticum aestivum). Genome mapping is based on direct visualization of sequence motifs on single DNA molecules hundreds of kilobases in length. With the genome map as a scaffold, we anchored unplaced sequence contigs, validated the initial draft assembly, and resolved instances of misassembly, some involving contigs <2 kb long, to dramatically improve the assembly from 75% to 95% complete. C1 [Hastie, Alex R.; Smith, Alexis; Finklestein, Jeff; Lam, Ernest T.; Cao, Han; Xiao, Ming] BioNano Genom, San Diego, CA USA. [Dong, Lingli; Huo, Naxin; Gu, Yong] ARS, Genom & Gene Discovery Res Unit, USDA, Albany, CA 94710 USA. [Dong, Lingli; Huo, Naxin; Deal, Karin R.; Dvorak, Jan; Luo, Ming-Cheng; Gu, Yong] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Kwok, Pui-Yan] Univ Calif San Francisco, Inst Human Genet, San Francisco, CA 94143 USA. RP Gu, Y (reprint author), ARS, Genom & Gene Discovery Res Unit, USDA, Albany, CA 94710 USA. EM yong.gu@ars.usda.gov; ming.xiao@drexel.edu RI Kwok, Pui-Yan/F-7725-2014; OI Kwok, Pui-Yan/0000-0002-5087-3059; Luo, Ming-Cheng/0000-0002-9744-5887 FU US National Institutes of Health (NIH) [R01 HG005946] FX This research is supported in part by US National Institutes of Health (NIH) award to P.-Y.K. and M. X. (R01 HG005946). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study. NR 36 TC 45 Z9 48 U1 4 U2 48 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2013 VL 8 IS 2 AR e55864 DI 10.1371/journal.pone.0055864 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 092WJ UT WOS:000315153400168 PM 23405223 ER PT J AU Merga, JY Williams, NJ Miller, WG Leatherbarrow, AJH Bennett, M Hall, N Ashelford, KE Winstanley, C AF Merga, J. Yvette Williams, Nicola J. Miller, William G. Leatherbarrow, Andrew J. H. Bennett, Malcolm Hall, Neil Ashelford, Kevin E. Winstanley, Craig TI Exploring the Diversity of Arcobacter butzleri from Cattle in the UK Using MLST and Whole Genome Sequencing SO PLOS ONE LA English DT Article ID MULTIPLEX PCR ASSAY; CAMPYLOBACTER SPP.; GENETIC DIVERSITY; POULTRY PRODUCTS; HEALTHY CATTLE; RETAIL MEATS; PREVALENCE; IDENTIFICATION; ANNOTATION; ARTEMIS AB Arcobacter butzleri is considered to be an emerging human foodborne pathogen. The completion of an A. butzleri genome sequence along with microarray analysis of 13 isolates in 2007 revealed a surprising amount of diversity amongst A. butzleri isolates from humans, animals and food. In order to further investigate Arcobacter diversity, 792 faecal samples were collected from cattle on beef and dairy farms in the North West of England. Arcobacter was isolated from 42.5% of the samples and the diversity of the isolates was investigated using multilocus sequence typing. An A. butzleri whole genome sequence, obtained by 454 shotgun sequencing of an isolate from a clinically-healthy dairy cow, showed a number of differences when compared to the genome of a human-derived A. butzleri isolate. PCR-based prevalence assays for variable genes suggested some tentative evidence for source-related distributions. We also found evidence for phenotypic differences relating to growth capabilities between our representative human and cattle isolates. Our genotypic and phenotypic observations suggest that some level of niche adaptation may have occurred in A. butzleri. C1 [Merga, J. Yvette; Williams, Nicola J.; Leatherbarrow, Andrew J. H.; Bennett, Malcolm; Winstanley, Craig] Univ Liverpool, Inst Infect & Global Hlth, Liverpool L69 3BX, Merseyside, England. [Miller, William G.] USDA, Produce Safety & Microbiol Res Unit, Albany, CA USA. [Hall, Neil; Ashelford, Kevin E.] Univ Liverpool, Inst Integrat Biol, Adv Genom Facil, Liverpool L69 3BX, Merseyside, England. RP Merga, JY (reprint author), Univ Liverpool, Inst Infect & Global Hlth, Liverpool L69 3BX, Merseyside, England. EM ysloane@liv.ac.uk OI Merga, Yvette/0000-0002-0692-1455; Hall, Neil/0000-0003-2808-0009 FU Higher Education Funding Council for England (HEFCE); Department for Environment, Food and Rural Affairs FX This work was funded by the Higher Education Funding Council for England (HEFCE) and the Department for Environment, Food and Rural Affairs, as part of the Veterinary Training and Research Initiative. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 38 TC 10 Z9 10 U1 1 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2013 VL 8 IS 2 AR e55240 DI 10.1371/journal.pone.0055240 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 092WJ UT WOS:000315153400071 PM 23405126 ER PT J AU Hagely, KB Palmquist, D Bilyeu, KD AF Hagely, Katherine B. Palmquist, Debra Bilyeu, Kristin D. TI Classification of Distinct Seed Carbohydrate Profiles in Soybean SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Soybean; carbohydrate; raffinose; stachyose; sucrose; galactinol ID LOW-RAFFINOSE; MODIFIED PHOSPHORUS; SUGAR CONTENT; STACHYOSE; OLIGOSACCHARIDES; MEAL; DIGESTIBILITIES; EMERGENCE; PHENOTYPE; GERMPLASM AB Soybeans are an important source of protein-rich meal for livestock feed formulations. Recent changes in the cost of commodity-based sources of metabolizable energy (ME) inputs has put pressure on soybean meal to deliver both protein and ME in feed formulations. The non-oil fraction of soybean contains approximately 1296 soluble carbohydrates, principally sucrose, raffinose, and stachyose. Of these carbohydrates, only sucrose is positive for ME. Both raffinose and stachyose, belonging to the raffinose family of oligosaccharides (RFOs), are considered antinutritional because of the negative consequences of their fermentation in the gut of monogastric animals when RFOs are consumed in the diet. Therefore, there is an interest in improving soybean seed composition so that it contains higher ME and fewer antinutritional components by increasing the sucrose content while lowering the RFOs. Several soybean lines have been discovered that contain altered levels of RFOs, and recent molecular genetic investigations have shown the phenotype to be caused by mutations in a raffinose synthase 2 (RS2) gene encoding the enzyme that is the committed step for RFO biosynthesis. The objective of this research was to determine the variation in carbohydrate profile for different soybean lines grown in a single location containing one of three different alleles of the RS2 gene. The results indicate that, although there is variation in the carbohydrate profiles for each line, different lines with the same RS2 genotype tend to produce a characteristic carbohydrate profile. Although the carbohydrate profile for each RS2 genotype class was consistent in different genetic backgrounds under two conditions grown at one location, more research will be necessary to determine the environmental stability of the carbohydrate profiles in multiple locations over different years. C1 [Hagely, Katherine B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Palmquist, Debra] ARS, USDA, Peoria, IL 61604 USA. [Bilyeu, Kristin D.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. RP Bilyeu, KD (reprint author), ARS, Plant Genet Res Unit, USDA, 110 Waters Hall, Columbia, MO 65211 USA. EM bilyeuk@missouri.edu NR 25 TC 9 Z9 9 U1 3 U2 48 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 6 PY 2013 VL 61 IS 5 BP 1105 EP 1111 DI 10.1021/jf303985q PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 087WL UT WOS:000314795000013 PM 23317449 ER PT J AU Brown, VL Drake, JM Stallknecht, DE Brown, JD Pedersen, K Rohani, P AF Brown, V. L. Drake, J. M. Stallknecht, D. E. Brown, J. D. Pedersen, K. Rohani, P. TI Dissecting a wildlife disease hotspot: the impact of multiple host species, environmental transmission and seasonality in migration, breeding and mortality SO JOURNAL OF THE ROYAL SOCIETY INTERFACE LA English DT Article DE avian influenza; multi-host mathematical model; environmental transmission; disease hotspot ID AVIAN INFLUENZA-VIRUS; DELAWARE BAY; A VIRUS; BIRDS; WATER; INFECTION; DYNAMICS; DUCKS; SURVEILLANCE; TEMPERATURE AB Avian influenza viruses (AIVs) have been implicated in all human influenza pandemics in recent history. Despite this, surprisingly little is known about the mechanisms underlying the maintenance and spread of these viruses in their natural bird reservoirs. Surveillance has identified an AIV 'hotspot' in shorebirds at Delaware Bay, in which prevalence is estimated to exceed other monitored sites by an order of magnitude. To better understand the factors that create an AIV hotspot, we developed and parametrized a mechanistic transmission model to study the simultaneous epizootiological impacts of multi-species transmission, seasonal breeding, host migration and mixed transmission routes. We scrutinized our model to examine the potential for an AIV hotspot to serve as a 'gateway' for the spread of novel viruses into North America. Our findings identify the conditions under which a novel influenza virus, if introduced into the system, could successfully invade and proliferate. C1 [Brown, V. L.; Rohani, P.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Brown, V. L.; Rohani, P.] Univ Michigan, Ctr Study Complex Syst, Ann Arbor, MI 48109 USA. [Drake, J. M.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Stallknecht, D. E.; Brown, J. D.] Univ Georgia, SE Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Pedersen, K.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Dis Program, Ft Collins, CO 80521 USA. [Rohani, P.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. RP Brown, VL (reprint author), Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. EM vlbrown@umich.edu RI Drake, John/D-6622-2012; OI Drake, John/0000-0003-4646-1235 FU James S. McDonnell Foundation; National Science Foundation [DEB-0917853]; RAPIDD program of the Science and Technology Directorate, Department of Homeland Security; Fogarty International Center, National Institutes of Health; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN266200700007C] FX This work was supported by the James S. McDonnell Foundation and the National Science Foundation (DEB-0917853). P.R. was also supported by the RAPIDD program of the Science and Technology Directorate, Department of Homeland Security, and the Fogarty International Center, National Institutes of Health. D. S. and J.B. were also supported by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under contract HHSN266200700007C. Data were collected by numerous biologists from state and federal agencies participating in the US Early Detection System for HPAI in wild birds, and made available through the USDA-APHIS Wildlife Services National Wildlife Disease Program. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of any of the funding agencies. NR 55 TC 7 Z9 7 U1 0 U2 204 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1742-5689 J9 J R SOC INTERFACE JI J. R. Soc. Interface PD FEB 6 PY 2013 VL 10 IS 79 AR 20120804 DI 10.1098/rsif.2012.0804 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 048VB UT WOS:000311940200009 PM 23173198 ER PT J AU Quadri, SS Stratford, RE Boue, SM Cole, RB AF Quadri, Syeda S. Stratford, Robert E. Boue, Stephen M. Cole, Richard B. TI Screening and Identification of Glyceollins and Their Metabolites by Electrospray Ionization Tandem Mass Spectrometry with Precursor Ion Scanning SO ANALYTICAL CHEMISTRY LA English DT Article ID PROSTATE-CANCER CELLS; FAST-ATOM-BOMBARDMENT; BREAST-CANCER; SOYBEAN GLYCEOLLINS; SOY; PHYTOALEXINS; GENISTEIN; ISOFLAVONOIDS; ASPERGILLUS; GLYCOSIDES AB A method has been developed for screening glyceollins and their metabolites based on precursor ion scanning. Under higher-energy collision conditions with the employment of a triple quadrupole mass spectrometer in the negative ion mode, deprotonated glyceollin precursors yield a diagnostic radical product ion at m/z 148. We propose this resonance-stabilized radical anion, formed in violation of the even-electron rule, to be diagnostic of glyceollins and glyceollin metabolites. Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) established that scanning for precursors of m/z 148 can identify glyceollins and their metabolites from plasma samples originating from rats dosed with glyceollins. Precursor peaks of interest were found at m/z 337, 353, 355, 417, and 433. The peak at m/z 337 corresponds to deprotonated glyceollins, whereas the others represent metabolites of glyceollins. Accurate mass measurement confirmed m/z 417 to be a sulfated metabolite of glyceollins. The peak at m/z 433 is also sulfated, but it contains an additional oxygen, as clonfirmed by accurate mass measurement. The latter metabolite differs from the former likely by the replacement of a hydrogen with a hydroxyl moiety. The peaks at m/z 353 and 355 are proposed to correspond to hydroxylated metabolites of glyceollins, wherein the latter additionally undergoes a double bond reduction. C1 [Quadri, Syeda S.; Cole, Richard B.] Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. [Stratford, Robert E.] Xavier Univ Louisiana, Coll Pharm, New Orleans, LA 70125 USA. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Cole, Richard B.] Univ Paris 06, Lab Chim Struct Organ & Biol, F-75252 Paris, France. RP Cole, RB (reprint author), Univ New Orleans, Dept Chem, 2000 Lakeshore Dr, New Orleans, LA 70148 USA. EM rcole@uno.edu FU National Science Foundation [CHE-1058764]; MetaboHUB project; Louisiana Cancer Research Consortium; NIH-RCMI Grant from the National Institute on Minority Health and Health Disparities [5G12RR026260] FX We thank Dr. Mark L. Heiman of NuMe Health for his expertise and input in designing the rat study, including identification of the vehicle. We thank Mr. Xiaohua Liu for performing accurate mass analyses by FT-ICR-MS. Financial support for this research was provided by the National Science Foundation through Grant CHE-1058764. Additional financial support was provided by the MetaboHUB project, the Louisiana Cancer Research Consortium, and the NIH-RCMI Grant 5G12RR026260 from the National Institute on Minority Health and Health Disparities. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the Louisiana Cancer Research Consortium or the NIH. NR 44 TC 8 Z9 8 U1 0 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD FEB 5 PY 2013 VL 85 IS 3 BP 1727 EP 1733 DI 10.1021/ac3030398 PG 7 WC Chemistry, Analytical SC Chemistry GA 086IA UT WOS:000314676100068 PM 23294002 ER PT J AU Lee, CC Braker, JD Grigorescu, AA Wagschal, K Jordan, DB AF Lee, Charles C. Braker, Jay D. Grigorescu, Arabela A. Wagschal, Kurt Jordan, Douglas B. TI Divalent metal activation of a GH43 beta-xylosidase SO ENZYME AND MICROBIAL TECHNOLOGY LA English DT Article DE beta-Xylosidase; Divalent cation; Hemicellulose; Multimerization ID SELENOMONAS-RUMINANTIUM; BIOCHEMICAL-CHARACTERIZATION; L-ARABINOFURANOSIDASE; ESCHERICHIA-COLI; CATALYTIC-PROPERTIES; PURIFICATION; CLONING; ARABINOSIDASE; DEGRADATION; INHIBITION AB Depolymerization of xylan, a major fraction of lignocellulosic biomass, releases xylose which can be converted into transportation fuels and chemical feedstocks. A requisite enzyme for the breakdown of xylan is beta-xylosidase. A gene encoding the 324-amino acid beta-xylosidase, RS223-BX, was cloned from an anaerobic mixed microbial culture. This glycoside hydrolase belongs to family 43. Unlike other GH43 enzymes, RS223-BX can be strongly activated by exogenously supplied Ca2+, Co2+, Fe2+, Mg2+, Mn2+ and Ni2+ (e.g., 28-fold by Mg2+) and it is inhibited by Cu2+ or Zn2+. Sedimentation equilibrium centrifugation experiments indicated that the divalent metal cations mediate multimerization of the enzyme from a dimeric to a tetrameric state, which have equal catalytic activity on an active-site basis. Compared to the determined active sites of other GH43 beta-xylosidases, the predicted active site of RS223-BX contains two additional amino acids with carboxylated side chains that provide potential sites for divalent metal cations to reside. Thus, the divalent metal cations likely occupy the active site and participate in the catalytic mechanism. RS223-BX accepts as substrate xylobiose, arabinobiose, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-alpha-L-arabinofuranoside. Additionally, the enzyme has good pH and temperature stabilities and a large K-i for D-glucose (1.3 M), favorable properties for performance in saccharification reactors. Published by Elsevier Inc. C1 [Lee, Charles C.; Wagschal, Kurt] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Braker, Jay D.; Jordan, Douglas B.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Grigorescu, Arabela A.] Northwestern Univ, Keck Biophys Facil, Evanston, IL 60201 USA. [Grigorescu, Arabela A.] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60201 USA. RP Lee, CC (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM Charles.Lee@ars.usda.gov RI Lee, Chung-Chieh/B-7238-2009 FU Cancer Center Grant [NCI CA060553] FX Sedimentation equilibrium experiments were conducted at Northwestern University, Keck Biophysics Facility, supported by a Cancer Center Grant (NCI CA060553). Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 39 TC 12 Z9 12 U1 3 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0141-0229 J9 ENZYME MICROB TECH JI Enzyme Microb. Technol. PD FEB 5 PY 2013 VL 52 IS 2 BP 84 EP 90 DI 10.1016/j.enzmictec.2012.10.010 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 082FW UT WOS:000314378700002 PM 23273276 ER PT J AU Lawrence, P Pacheco, JM Uddowla, S Hollister, J Kotecha, A Fry, E Rieder, E AF Lawrence, Paul Pacheco, Juan M. Uddowla, Sabena Hollister, Jason Kotecha, Abhay Fry, Elizabeth Rieder, Elizabeth TI Foot-and-mouth disease virus (FMDV) with a stable FLAG epitope in the VP1 G-H loop as a new tool for studying FMDV pathogenesis SO VIROLOGY LA English DT Article DE Foot-and-mouth disease virus (FMDV); Pathogenesis; FLAG tag ID SURFACE HEPARAN-SULFATE; ANTIGENIC SITES; INTEGRIN ALPHA(V)BETA(3); NEUTRALIZING EPITOPES; MONOCLONAL-ANTIBODIES; MAMMALIAN-CELLS; RECEPTOR; PROTEIN; BINDING; CATTLE AB Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and growth kinetics to parental virus. A24-FLAG was distinguished, neutralized, and immunoprecipitated by FLAG anti-sera. A24-FLAG infected cattle exhibited FMD and an antibody response similar to parental virus. FLAG epitope stability was confirmed both in vitro and in vivo. Interestingly, no anti-FLAG antibodies were detectable in cattle up to 21 days post-inoculation. A24-FLAG G-H loop modeling suggested FLAG was rendered a cryptic site, inaccessible to the host immune system. These studies demonstrate the FMDV VP1 G-H loop tolerance to substitutions without detriment to pathogenesis and antigenicity. Finally, A24-FLAG manifested virulence in cattle as parental virus, and could be distinguished and tracked by tag-specific anti-sera. Published by Elsevier Inc. C1 [Lawrence, Paul; Pacheco, Juan M.; Uddowla, Sabena; Hollister, Jason; Rieder, Elizabeth] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Kotecha, Abhay; Fry, Elizabeth] Univ Oxford, Nuffield Dept Clin Med, Div Struct Biol, Oxford OX3 7BN, England. RP Rieder, E (reprint author), ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM elizabeth.rieder@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201 FU Plum Island Animal Disease Research Participation Program FX Dr. Paul Lawrence and Dr. Sabena Uddowla were sponsored by the Plum Island Animal Disease Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture. We wish to thank both Lisa Aschenbrenner and Elizabeth A. Schafer for their skillful expertise during the plasmid construction phase of this project as well as fruitful discussions. We also wish to thank Michael LaRocco, Joseph Conderino, Ethan Hartwig, and Elizabeth Bishop for their assistance with the in vitro diagnostic assay and the downstream assays after animal infection, respectively. We also wish to thank Jingshan Ren for helpful discussions. NR 67 TC 6 Z9 9 U1 1 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD FEB 5 PY 2013 VL 436 IS 1 BP 150 EP 161 DI 10.1016/j.virol.2012.11.001 PG 12 WC Virology SC Virology GA 077BV UT WOS:000314003800018 PM 23228860 ER PT J AU Temeyer, KB Brake, DK Tuckow, AP Li, AY de Leon, AAP AF Temeyer, Kevin B. Brake, Danett K. Tuckow, Alexander P. Li, Andrew Y. de Leon, Adalberto A. Perez TI Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): cDNA sequence, baculovirus expression, and biochemical properties SO PARASITES & VECTORS LA English DT Article DE Sand fly; Acetylcholinesterase; P. papatasi; cDNA; AChE ID ORGANOPHOSPHATE-INSENSITIVE ACETYLCHOLINESTERASE; ZOONOTIC CUTANEOUS LEISHMANIASIS; AMINO-ACID SUBSTITUTION; COLORADO-POTATO BEETLE; TALLIL AIR BASE; INSECTICIDE RESISTANCE; MILITARY OPERATIONS; POINT MUTATIONS; RESERVOIR HOST; L. DIPTERA AB Background: Millions of people and domestic animals around the world are affected by leishmaniasis, a disease caused by various species of flagellated protozoans in the genus Leishmania that are transmitted by several sand fly species. Insecticides are widely used for sand fly population control to try to reduce or interrupt Leishmania transmission. Zoonotic cutaneous leishmaniasis caused by L. major is vectored mainly by Phlebotomus papatasi (Scopoli) in Asia and Africa. Organophosphates comprise a class of insecticides used for sand fly control, which act through the inhibition of acetylcholinesterase (AChE) in the central nervous system. Point mutations producing an altered, insensitive AChE are a major mechanism of organophosphate resistance in insects and preliminary evidence for organophosphate-insensitive AChE has been reported in sand flies. This report describes the identification of complementary DNA for an AChE in P. papatasi and the biochemical characterization of recombinant P. papatasi AChE. Methods: A P. papatasi Israeli strain laboratory colony was utilized to prepare total RNA utilized as template for RT-PCR amplification and sequencing of cDNA encoding acetylcholinesterase 1 using gene specific primers and 3'-5'-RACE. The cDNA was cloned into pBlueBac4.5/V5-His TOPO, and expressed by baculovirus in Sf21 insect cells in serum-free medium. Recombinant P. papatasi acetylcholinesterase was biochemically characterized using a modified Ellman's assay in microplates. Results: A 2309 nucleotide sequence of PpAChE1 cDNA [GenBank: JQ922267] of P. papatasi from a laboratory colony susceptible to insecticides is reported with 73-83% nucleotide identity to acetylcholinesterase mRNA sequences of Culex tritaeniorhynchus and Lutzomyia longipalpis, respectively. The P. papatasi cDNA ORF encoded a 710-amino acid protein [GenBank: AFP20868] exhibiting 85% amino acid identity with acetylcholinesterases of Cx. pipiens, Aedes aegypti, and 92% amino acid identity for L. longipalpis. Recombinant P. papatasi AChE1 was expressed in the baculovirus system and characterized as an insect acetylcholinesterase with substrate preference for acetylthiocholine and inhibition at high substrate concentration. Enzyme activity was strongly inhibited by eserine, BW284c51, malaoxon, and paraoxon, and was insensitive to the butyrylcholinesterase inhibitors ethopropazine and iso-OMPA. Conclusions: Results presented here enable the screening and identification of PpAChE mutations resulting in the genotype for insensitive PpAChE. Use of the recombinant P. papatasi AChE1 will facilitate rapid in vitro screening to identify novel PpAChE inhibitors, and comparative studies on biochemical kinetics of inhibition. C1 [Temeyer, Kevin B.; Brake, Danett K.; Tuckow, Alexander P.; Li, Andrew Y.; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Temeyer, KB (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM Kevin.Temeyer@ars.usda.gov FU U.S. Department of Agriculture [USDA] appropriated funds (USDA-ARS CRIS) [6205-32000-033000D]; USDA-ARS CRADA [60-0208-9-601, 6201-32000-033017R] FX The authors thank Kristie Schlechte, DeEsta Hyatt and Darci Burchers for excellent technical assistance. The authors also thank Dr. Fan Tong and Dr. Donald Thomas for critical review of the manuscript. This work was funded by U.S. Department of Agriculture [USDA] appropriated funds (USDA-ARS CRIS number 6205-32000-033000D, Molecular biology and physiology of biting flies affecting livestock) and USDA-ARS CRADA number 60-0208-9-601 (Project number 6201-32000-033017R, Deployed Warfighter Protection Research Program [DWFP] of the U.S. Department of Defense through the Armed Forces Pest Management Board [AFPMB]). NR 53 TC 7 Z9 7 U1 2 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 4 PY 2013 VL 6 AR 31 DI 10.1186/1756-3305-6-31 PG 8 WC Parasitology SC Parasitology GA 103KC UT WOS:000315913800001 PM 23379291 ER PT J AU Zanon-Moreno, V Asensio-Marquez, EM Ciancotti-Oliver, L Garcia-Medina, JJ Sanz, P Ortega-Azorin, C Pinazo-Duran, MD Ordovas, JM Corella, D AF Zanon-Moreno, Vicente Asensio-Marquez, Eva M. Ciancotti-Oliver, Lucia Garcia-Medina, Jose J. Sanz, Pedro Ortega-Azorin, Carolina Pinazo-Duran, Maria D. Ordovas, Jose M. Corella, Dolores TI Effects of polymorphisms in vitamin E-, vitamin C-, and glutathione peroxidase-related genes on serum biomarkers and associations with glaucoma SO MOLECULAR VISION LA English DT Article ID OPEN-ANGLE GLAUCOMA; TOCOPHEROL TRANSFER PROTEIN; GENOME-WIDE ASSOCIATION; ALPHA-TOCOPHEROL; OXIDATIVE STRESS; OSTEOPOROTIC FRACTURES; ASCORBIC-ACID; CELL-DEATH; RISK; VARIANTS AB Purpose: To study the association of selected polymorphism in genes related to vitamin E, vitamin C, and glutathione peroxidase with these biomarkers and primary open-angle glaucoma (POAG) risk. Methods: A case-control study matched for age, sex, and bodyweight was undertaken. Two hundred fifty POAG cases and 250 controls were recruited from a Mediterranean population. Plasma concentrations of vitamin C, vitamin E, and glutathione peroxidase (GPx) activity were measured. We analyzed the polymorphisms rs1279683 in the Na+-dependent L-ascorbic acid transporter 2 (SLC23A2) gene, rs6994076 in the tocopherol alpha transfer protein (TTPA) gene, rs737723 in the tocopherol-associated protein (SEC14L2/TAP) gene, and rs757228 in the glutathione peroxidase 4 (GPX4) gene. We also analyzed expression of the SLC23A2 gene in a subsample. Results: We found a novel association between the rs737723 polymorphism and POAG risk. Homozygous subjects for the C allele had a higher POAG risk than carriers of the ancestral G allele (adjusted odds ratio 1.73, 95% confidence interval 1.13-2.65, p=0.011). This association remained statistically significant after adjustment for multiple comparisons. We also confirmed the association between the rs1279683 polymorphism and a higher POAG risk in GG homozygous subjects and detected statistically significant differences in SLC23A2 gene expression between POAG cases and controls, even after adjustment for multiple testing. We observed a nominally significant (p<0.05) gene-gene interaction between the SEC14L2/TAP and SLC23A2 polymorphisms in determining POAG risk, increasing POAG risk in those subjects who had both risk genotypes at the same time (p<0.01). This increase was statistically significant even after adjustment for multiple comparisons. We did not detect any association with POAG risk for the rs6994076 or rs757228 polymorphisms. We also found that POAG patients had statistically significant (after correction for multiple testing) lower plasma vitamin E (p<0.001) and vitamin C (p<0.001) concentrations than control subjects. However, we detected a higher plasma GPx activity in POAG cases than in controls (p<0.001). The rs6994076 and rs1279683 polymorphisms were significantly (p<0.001) associated with plasma vitamin E and vitamin C, respectively. However, the rs757228 polymorphism in the GPX4 gene was not associated with plasma GPx activity. Conclusions: We have described a novel association between the rs737723 polymorphism (SEC14L2/TAP) and higher POAG risk and confirmed the association between rs1279683 (SLC23A2) and POAG. Our results also suggested a gene-gene interaction between both polymorphisms that increases POAG risk. C1 [Zanon-Moreno, Vicente; Asensio-Marquez, Eva M.; Ortega-Azorin, Carolina; Corella, Dolores] Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Genet & Mol Epidemiol Unit, Valencia 46010, Spain. [Zanon-Moreno, Vicente; Asensio-Marquez, Eva M.; Ortega-Azorin, Carolina; Corella, Dolores] Univ Valencia, CIBER Fisiopatol Obesidad & Nutr, Valencia 46010, Spain. [Ciancotti-Oliver, Lucia] Dr Peset Univ Hosp, Dept Prevent Med & Publ Hlth, Valencia, Spain. [Garcia-Medina, Jose J.] Reina Sofia Univ Gen Hosp, Dept Ophthalmol, Murcia, Spain. [Garcia-Medina, Jose J.; Sanz, Pedro; Pinazo-Duran, Maria D.] Dr Peset Univ Hosp, Ophthalmol Res Unit Santiago Grisolia, Valencia, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, Jose M.] IMDEA, Madrid, Spain. RP Zanon-Moreno, V (reprint author), Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Genet & Mol Epidemiol Unit, Avda Blasco Ibanez 15, Valencia 46010, Spain. EM Vicente.Zanon-Moreno@uv.es OI Zanon-Moreno, Vicente/0000-0003-1179-1592; Garcia Medina, Jose Javier/0000-0002-6245-7271 FU Ministerio de Ciencia e Innovacion; Fondo Europeo de Desarrollo Regional (FEDER) [CIBER CB06/03/0035]; Postdoctoral contract 'Sara Borrell', Instituto de Salud Carlos III, Madrid [CD09/00437]; Generalitat Valenciana, Spain [GVACOMP2011-151, AP111/10]; US Department of Agriculture Research [53-K06-5-10, 58-1950-9-001] FX This work was supported by grants from the Ministerio de Ciencia e Innovacion and Fondo Europeo de Desarrollo Regional (FEDER): CIBER CB06/03/0035; Postdoctoral contract 'Sara Borrell', Instituto de Salud Carlos III (CD09/00437), Madrid; the Generalitat Valenciana, Spain (GVACOMP2011-151, AP111/10) and by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research. NR 55 TC 9 Z9 9 U1 0 U2 5 PU MOLECULAR VISION PI ATLANTA PA C/O JEFF BOATRIGHT, LAB B, 5500 EMORY EYE CENTER, 1327 CLIFTON RD, N E, ATLANTA, GA 30322 USA SN 1090-0535 J9 MOL VIS JI Mol. Vis. PD FEB 3 PY 2013 VL 19 BP 231 EP 242 PG 12 WC Biochemistry & Molecular Biology; Ophthalmology SC Biochemistry & Molecular Biology; Ophthalmology GA 089LC UT WOS:000314911000001 PM 23401652 ER PT J AU Ager, AA Vaillant, NM McMahan, A AF Ager, Alan A. Vaillant, Nicole M. McMahan, Andrew TI Restoration of fire in managed forests: a model to prioritize landscapes and analyze tradeoffs SO ECOSPHERE LA English DT Article DE dry forests; forest management; forest restoration; fuel treatment; spatial optimization; wildfire hazard; wildfire risk ID WESTERN UNITED-STATES; SOUTHWESTERN PONDEROSA PINE; FUEL TREATMENTS; ECOLOGICAL RESTORATION; CONSERVATION BIOLOGY; SPATIAL-PATTERNS; LARGE WILDFIRES; TIMBER HARVEST; CENTRAL OREGON; NORTH-AMERICA AB Ongoing forest restoration on public lands in the western US is a concerted effort to counter the growing incidence of uncharacteristic wildfire in fire-adapted ecosystems. Restoration projects cover 725,000 ha annually, and include thinning and underburning to remove ladder and surface fuel, and seeding of fire-adapted native grasses and shrubs. The backlog of areas in need of restoration combined with limited budgets requires that projects are implemented according to a prioritization system. The current system uses a stand-scale metric that measures ecological departure from pre-settlement conditions. Although conceptually appealing, the approach does not consider important spatial factors that influence both the efficiency and feasibility of managing future fire in the post-treatment landscape. To address this gap, we developed a spatial model that can be used to explore different landscape treatment configurations and identify optimal project parameters that maximize restoration goals. We tested the model on a 245,000 ha forest and analyzed tradeoffs among treatment strategies as defined by fire behavior thresholds, total area treated, and the proportion of the project area treated. We assumed the primary goal as the protection and conservation of old growth ponderosa pine trees from potential wildfire loss. The model located optimal project areas for restoration and identified treatment areas within them, although the location was dependent on assumptions about acceptable fire intensity within restored landscapes, and the total treated area per project. When a high percentage of stands was treated (e. g., >80%), the resulting project area was relatively small, leaving the surrounding landscape at risk for fire. Conversely, treating only a few stands with extreme fire behavior (<20%) created larger projects, but substantial old growth forests remained susceptible to wildfire mortality within the project area. Intermediate treatment densities (35%) were optimal in terms of the overall reduction in the potential wildfire mortality of old growth. The current work expands the application in spatial optimization to the problem of dry forest restoration, and demonstrates a decision support protocol to prioritize landscapes and specific areas to treat within them. The concepts and model can be applied to similar problems in spatial ecology. C1 [Ager, Alan A.; Vaillant, Nicole M.] US Forest Serv, USDA, Pacific NW Res Stn, Prineville, OR 97754 USA. [McMahan, Andrew] Softec Solut, Ft Collins, CO 80526 USA. RP Ager, AA (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Prineville, OR 97754 USA. EM aager@fs.fed.us FU USFS National Fire Decision Support Center FX We thank Dana Simon of the Deschutes National Forest for GIS support. Brian Tandy, Pete Powers, Dave Owens, Bob Clements and a number of other Forest Service fuel planners provided feedback on concepts presented in the paper. Michelle Buonopane provided technical support and prepared many of the figures. The USFS National Fire Decision Support Center contributed funding for the study. We are grateful to John Lehmkuhl and anonymous reviewers for valuable comments on earlier versions of the manuscript. NR 60 TC 14 Z9 14 U1 8 U2 32 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2013 VL 4 IS 2 AR UNSP 29 DI 10.1890/ES13-00007.1 PG 19 WC Ecology SC Environmental Sciences & Ecology GA 256JN UT WOS:000327306400013 ER PT J AU Cuddington, K Fortin, MJ Gerber, LR Hastings, A Liebhold, A O'Connor, M Ray, C AF Cuddington, K. Fortin, M. -J. Gerber, L. R. Hastings, A. Liebhold, A. O'Connor, M. Ray, C. TI Process-based models are required to manage ecological systems in a changing world SO ECOSPHERE LA English DT Article DE climate change; expert opinion; extrapolation; simulation model ID CLIMATE-CHANGE; FOREST MANAGEMENT; PREDICTIVE MODELS; GLOBAL CHANGE; DYNAMICS; IMPACTS; CONSERVATION; POPULATIONS; SCALE; UNCERTAINTIES AB Several modeling approaches can be used to guide management decisions. However, some approaches are better fitted than others to address the problem of prediction under global change. Process-based models, which are based on a theoretical understanding of relevant ecological processes, provide a useful framework to incorporate specific responses to altered environmental conditions. As a result, these models can offer significant advantages in predicting the effects of global change as compared to purely statistical or rule-based models based on previously collected data. Process-based models also offer more explicitly stated assumptions and easier interpretation than detailed simulation models. We provide guidelines for identifying the appropriate type of model and level of complexity for management decisions. Finally we outline some of those factors that make modeling for local and regional management under global change a particular challenge: changes to relevant scales and processes, additional sources of uncertainty, legacy effects, threshold dynamics, and socio-economic impacts. C1 [Cuddington, K.] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada. [Fortin, M. -J.] Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 3G5, Canada. [Gerber, L. R.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Hastings, A.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Liebhold, A.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. [O'Connor, M.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada. [Ray, C.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. RP Cuddington, K (reprint author), Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada. EM kcudding@uwaterloo.ca RI O'Connor, Mary/F-2275-2010; OI RAY, CHRIS/0000-0002-7963-9637 FU University of California-Davis; NSF [DEB-06-40021] FX This work arose out of a group meeting sponsored by the University of California-Davis, and an NSF award # DEB-06-40021 to Alan Hastings. NR 62 TC 19 Z9 20 U1 4 U2 33 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2013 VL 4 IS 2 AR UNSP 20 DI 10.1890/ES12-00178.1 PG 12 WC Ecology SC Environmental Sciences & Ecology GA 256JN UT WOS:000327306400004 ER PT J AU Inman-Narahari, F Ostertag, R Cordell, S Giardina, CP Nelson-Kaula, K Sack, L AF Inman-Narahari, Faith Ostertag, Rebecca Cordell, Susan Giardina, Christian P. Nelson-Kaula, Kehauwealani Sack, Lawren TI Seedling recruitment factors in low-diversity Hawaiian wet forest: towards global comparisons among tropical forests SO ECOSPHERE LA English DT Article DE Center for Tropical Forest Science; Cheirodendron trigynum; Coprosma rhynchocarpa; dispersal limitation; establishment limitation; habitat association; Hawaii, USA; Metrosideros polymorpha; Pianka's niche overlap; regeneration ecology; seed limitation ID NET ECOSYSTEM EXCHANGE; BORNEAN RAIN-FOREST; HABITAT ASSOCIATIONS; NEOTROPICAL FOREST; NICHE OVERLAP; DISPERSAL LIMITATION; REGENERATION NICHE; LIGHT AVAILABILITY; ADAPTIVE RADIATION; SPECIES-DIVERSITY AB Recruitment limitations determine forest community regeneration patterns. Source limitation and dispersal limitation contribute to overall seed limitation, while environmental conditions and habitat associations influence establishment limitation. Several hypotheses have made contradictory predictions for how the relative importance of these limitations should vary with diversity. However, comparative data have not been available for low-diversity tropical forests. We quantified recruitment limitations using 2.5 yrs of seed rain and seedling distribution data collected within a 4 ha forest dynamics plot in low-diversity native-dominated Hawaiian wet forest. We further quantified seedling irradiance and substrate habitat associations and niche overlap (using Pianka's niche overlap index). Additionally, we compared recruitment limitations and the frequency of seedling habitat associations across forests using the few available published data from sites employing similar field and analytical methods. In Hawaiian wet forest, seed dispersal more strongly limited recruitment than did establishment limitation across species, with 11 of 18 species completely seed limited (i.e., no seeds found). However, the relative importance of limitations varied greatly among species. For the three most abundant species, habitat conditions more strongly limited regeneration than did seed arrival, especially for the dominant canopy species, Metrosideros polymorpha, which was not seed limited. Most species were significantly associated with specific ranges of irradiance and/or substrates. Although habitat associations may indicate niche differentiation, Hawaiian species also showed significant niche overlap. Across the three forests compared, community-wide mean seed and establishment limitation values were similar, despite wide variation in diversity. However, recruitment limitations differed strongly among species within forests due to species' life-history differences. While seed limitation in Hawaiian forest was as high as in high-diversity forests, mechanisms may differ; seed limitation in Hawaii may arise from loss of pollinators and dispersers rather than from a high proportion of rare species as occurs in high-diversity forests. The strong habitat associations in Hawaiian forest relative to high-diversity forests supported theoretical expectations that lower species diversity should increase the importance of habitat associations. However, these habitat associations were not linked to niche differentiation in Hawaii. Our findings suggest that high recruitment limitation may facilitate coexistence despite niche overlap in low-diversity Hawaiian forest. C1 [Inman-Narahari, Faith; Sack, Lawren] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. [Ostertag, Rebecca; Nelson-Kaula, Kehauwealani] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [Cordell, Susan; Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, Hilo, HI 96720 USA. RP Inman-Narahari, F (reprint author), Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. EM ohia@ucla.edu RI Giardina, Christian/C-3120-2011; Sack, Lawren/A-5492-2008 OI Giardina, Christian/0000-0002-3431-5073; Sack, Lawren/0000-0002-7009-7202 FU National Science Foundation [EPSCoR 0554657, IOS-0546784]; Smithsonian Tropical Research Institute Center for Tropical Forest Science; University of California, Los Angeles; University of Hawaii; USDA Forest Service Pacific Southwest Research Station Institute of Pacific Islands Forestry (USFS-IPIF) FX We thank the many field assistants who collected seedling and seed rain data, including Molly Murphy, Kahealani Wailani-Nihipali and others with the Hawaii Community College Tropical Forest Ecosystem and Agroforestry Management and the University of Hawaii at Hilo Pacific Internship Programs for Exploring Science programs; Bernice Hwang, Michael Nullet, Paul Scowcroft and Jodie Schulten for assistance with logistics; and the National Science Foundation (Grants EPSCoR 0554657 and IOS-0546784), the Smithsonian Tropical Research Institute Center for Tropical Forest Science; the University of California, Los Angeles, the University of Hawaii, and the USDA Forest Service Pacific Southwest Research Station Institute of Pacific Islands Forestry (USFS-IPIF), for financial and other support. This project was conducted within the Hawaii Experimental Tropical Forest, managed collaboratively by the USFS-IPIF and the Hawaii Division of Forestry and Wildlife/Department of Land and Natural Resources. NR 102 TC 7 Z9 7 U1 7 U2 32 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2013 VL 4 IS 2 AR UNSP 24 DI 10.1890/ES12-00164.1 PG 19 WC Ecology SC Environmental Sciences & Ecology GA 256JN UT WOS:000327306400008 ER PT J AU Richardson, L Loomis, JB Champ, PA AF Richardson, Leslie Loomis, John B. Champ, Patricia A. TI Valuing Morbidity from Wildfire Smoke Exposure: A Comparison of Revealed and Stated Preference Techniques SO LAND ECONOMICS LA English DT Article ID WILLINGNESS-TO-PAY; CONTINGENT VALUATION SURVEYS; SIMULATED LIKELIHOOD ESTIMATION; CONFIDENCE-INTERVALS; REDUCED MORBIDITY; AIR-POLLUTION; HEALTH; BENEFITS; COST; CALIFORNIA AB Estimating the economic benefits of reduced health damages due to improvements in environmental quality continues to challenge economists. We review welfare measures associated with reduced wildfire smoke exposure, and a unique dataset from California's Station Fire of 2009 allows for a comparison of cost of illness (COI) estimates with willingness to pay (WTP) measures. The WTP for one less symptom day is estimated to be $87 and $95, using the defensive behavior and contingent valuation methods, respectively. These WTP estimates are not statistically different but do differ from a $3 traditional daily COI estimate and $17 comprehensive daily COI estimate. C1 [Richardson, Leslie] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO USA. [Loomis, John B.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [Champ, Patricia A.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. RP Richardson, L (reprint author), US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO USA. NR 67 TC 4 Z9 4 U1 3 U2 18 PU UNIV WISCONSIN PRESS PI MADISON PA JOURNAL DIVISION, 1930 MONROE ST, 3RD FL, MADISON, WI 53711 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD FEB PY 2013 VL 89 IS 1 BP 76 EP 100 PG 25 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA 245QM UT WOS:000326478900005 ER PT J AU Matthan, NR Zhu, L Pencina, M D'Agostino, RB Schaefer, EJ Lichtenstein, AH AF Matthan, Nirupa R. Zhu, Lei Pencina, Michael D'Agostino, Ralph B. Schaefer, Ernst J. Lichtenstein, Alice H. TI Sex-Specific Differences in the Predictive Value of Cholesterol Homeostasis Markers and 10-Year Cardiovascular Disease Event Rate in Framingham Offspring Study Participants SO JOURNAL OF THE AMERICAN HEART ASSOCIATION LA English DT Article DE cardiovascular disease; lipids; metabolism; mortality; myocardial infarction; risk factors ID CORONARY-HEART-DISEASE; DENSITY-LIPOPROTEIN CHOLESTEROL; SERUM PLANT STEROLS; ARTERY-DISEASE; POSTMENOPAUSAL WOMEN; METABOLIC SYNDROME; PLASMA-LEVELS; NONCHOLESTEROL STEROLS; ABSORPTION EFFICIENCY; INSULIN-RESISTANCE AB Background-Available data are inconsistent regarding factors influencing plasma cholesterol homeostasis marker concentrations and their value in predicting subsequent cardiovascular disease (CVD) events. Methods and Results-To address this issue, the relationship between markers of cholesterol absorption (campesterol, sitosterol, cholestanol) and synthesis (squalene, desmosterol, lathosterol) and 10-year CVD incidence was assessed in Framingham Offspring Study participants (cycle 6) who were without CVD at baseline and not taking lipid-lowering medications (N=2616). The primary end point was "hard" coronary heart disease (HCHD; coronary death and myocardial infarction), and the secondary end point was full CVD (HCHD plus stroke, coronary insufficiency, angina pectoris, peripheral artery disease, and congestive heart failure). In cross-sectional analysis, significant differences by sex, age, body mass index, blood pressure, and smoking status were observed. In both women and men, lower cholesterol absorption was associated with higher triglyceride and lower high-density lipoprotein (HDL) cholesterol concentrations, whereas lower cholesterol synthesis was associated with higher low-density lipoprotein (LDL) cholesterol concentrations (P for trend < 0.05). In women only, lower cholesterol synthesis and absorption were associated with higher non-HDL cholesterol concentrations. Using Cox proportional hazards model adjusting for standard CVD risk factors, squalene concentrations were associated with lower HCHD in women (hazard ratio=0.70 [0.5 to 0.9]). In contrast, squalene (hazard ratio=1.40 [1.1 to 1.8]) concentrations were associated with higher HCHD in men (P<0.0001 for interaction). The cholesterol absorption markers were not predictive of HCHD or full CVD in either women or men. Conclusions-These data suggest significant sex differences in the 10-year prognostic value of cholesterol synthesis markers and HCHD, specifically coronary death and incidence of myocardial infarction. C1 [Matthan, Nirupa R.; Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Schaefer, Ernst J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. [Zhu, Lei; Pencina, Michael; D'Agostino, Ralph B.] Boston Univ, Dept Biostat, Boston, MA 02215 USA. RP Matthan, NR (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM nirupa.matthan@tufts.edu FU National Institutes of Health [HL 074388, N01-HC-25195]; United States Department of Agriculture [58-1950-4-401] FX This work was supported by grants HL 074388 (N.R.M., A. H. L., E.J.S.) and N01-HC-25195 (L.Z., M. P., R. B. D.) from the National Institutes of Health and the United States Department of Agriculture, under agreement No. 58-1950-4-401. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 58 TC 14 Z9 14 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-9980 J9 J AM HEART ASSOC JI J. Am. Heart Assoc. PD FEB PY 2013 VL 2 IS 1 AR UNSP e005066 DI 10.1161/JAHA.112.005066 PG 13 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 243SF UT WOS:000326336800026 PM 23525441 ER PT J AU Boyette, CD Hoagland, RE AF Boyette, C. Douglas Hoagland, Robert E. TI Bioherbicidal potential of a strain of Xanthomonas spp. for control of common cocklebur (Xanthium strumarium) SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE bioherbicide; biological control; Xanthomonas spp; cocklebur; Xanthium strumarium ID SOYBEAN GLYCINE-MAX; FUNGUS COLLETOTRICHUM-GLOEOSPORIOIDES; SESBANIA SESBANIA-EXALTATA; BIOLOGICAL-CONTROL; ALTERNARIA-HELIANTHI; WEED MANAGEMENT; INVERT EMULSION; POAE JT-P482; INTERFERENCE; RESISTANT AB Several isolates of a previously unreported bacterial pathogen were discovered on common cocklebur seedlings in Chicot County, AR and Washington County, MS. Diseased plants in nature exhibited angular-shaped leaf spotting symptoms on leaf margins and central leaf areas. The isolates were cultured from diseased leaf tissue and tentatively identified as Xanthomonas spp., and their virulence on common cocklebur seedlings compared. The most virulent isolate (LVA987) was used in studies to define disease progression on cocklebur seedlings and to carry out a host range evaluation on various weeds and crop plants. High virulence was found on common cocklebur > marestail (Conyza canadensis) > giant ragweed (Ambrosia trifida) and common ragweed (Ambrosia artemisifolia). These results suggest this pathogen may be useful for the biological control of these important species of weeds. This is also highly relevant since all of these weeds have evolved resistance to one or more synthetic herbicides and are thus becoming more difficult to control with conventional herbicides. C1 [Boyette, C. Douglas] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Hoagland, Robert E.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Boyette, CD (reprint author), USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM doug.boyette@ars.usda.gov NR 64 TC 1 Z9 1 U1 3 U2 9 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD FEB 1 PY 2013 VL 23 IS 2 BP 183 EP 196 DI 10.1080/09583157.2012.745485 PG 14 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 243XH UT WOS:000326351200005 ER PT J AU Meikle, WG Bon, MC Cook, SC Gracia, C Jaronski, ST AF Meikle, William G. Bon, Marie-Claude Cook, Steve C. Gracia, Carlos Jaronski, Stefan T. TI Two strains of Pseudomonas fluorescens bacteria differentially affect survivorship of waxworm (Galleria mellonella) larvae exposed to an arthropod fungal pathogen, Beauveria bassiana SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE biopesticide; microbial contamination; Galleria mellonella; entomopathogenic fungus ID HONEY-BEE HYMENOPTERA; ENTOMOPATHOGENIC FUNGUS; VARROA MITES; ACARI; DEUTEROMYCOTA; GROWTH; CONTAMINATION; BIOPESTICIDE; TEMPERATURE; MANAGEMENT AB Two strains of Pseudomonas fluorescens were found contaminating a biopesticide used in a previous study against Varroa destructor infestations in honey bee hives. In that study, the biopesticide, a formulation of a fungal pathogen of arthropods, Beauveria bassiana, failed to have any negative impact on the mite infestation despite successful results in previous studies using uncontaminated batches of the same biopesticide. The objective of the present research was to determine whether the bacteria may have interfered with the infectivity and/or virulence of B. bassiana in a simplified system; positive results in that system would then provide a rationale for further work under more complex conditions. Galleria mellonella late instar larvae treated topically with both a bacterial suspension of 6.8 to 7.0x10(7) cfu/ml and a fungal suspension of 2.5x10(7) or 2.5x10(8)B. bassiana conidia/ml showed, in the case of one of the bacterial strains, significantly increased survivorship compared to larvae treated with just the B. bassiana suspension. When larvae were immersed in a bacterial suspension prior to application of B. bassiana suspension using a spray tower, a significant positive effect of the same P. fluorescens strain on larval survivorship was observed at 2.5x10(8) conidia/ml. Neither the bacterial suspensions alone nor blank control solutions had any effect on larval survivorship. These results show that an interaction between the bacteria and the pathogen may explain some of the results from the prior field trial. C1 [Meikle, William G.; Cook, Steve C.; Gracia, Carlos] USDA, ARS, Honey Bee Res Unit, Weslaco, TX USA. [Bon, Marie-Claude] USDA, ARS, European Biol Control Lab, Montferrier Sur Lez, France. [Jaronski, Stefan T.] USDA, ARS, Northern Plains Agr Res Lab, Sidney, BC, Canada. RP Meikle, WG (reprint author), USDA, ARS, Honey Bee Res Unit, Weslaco, TX USA. EM william.meikle@ars.usda.gov NR 28 TC 0 Z9 0 U1 1 U2 13 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD FEB 1 PY 2013 VL 23 IS 2 BP 220 EP 233 DI 10.1080/09583157.2012.753406 PG 14 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 243XH UT WOS:000326351200008 ER PT J AU Kalchayanand, N Arthur, TM Bosilevac, JM Wells, JE Wheeler, TL AF Kalchayanand, Norasak Arthur, Terrance M. Bosilevac, Joseph M. Wells, James E. Wheeler, Tommy L. TI Chromogenic Agar Medium for Detection and Isolation of Escherichia coli Serogroups O26, O45, O103, O111, O121, and O145 from Fresh Beef and Cattle Feces SO JOURNAL OF FOOD PROTECTION LA English DT Article ID AUTOMATED IMMUNOMAGNETIC SEPARATION; MULTIPLEX PCR; UNITED-STATES; GENE-CLUSTER; GROUND-BEEF; O157; ANIMALS; IDENTIFICATION; SALMONELLA; PREVALENCE AB Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains are clinically important foodborne pathogens. Unlike E. coli O157:H7, these foodborne pathogens have no unique biochemical characteristics to readily distinguish them from other E. coli strains growing on plating media. In this study, a chromogenic agar medium was developed in order to differentiate among non-O157 STEC strains of serogroups O26, O45, O103, O111, O121, and O145 on a single agar medium. The ability of this chromogenic agar medium to select and distinguish among these pathogens is based on a combination of utilization of carbohydrates, b-galactosidase activity, and resistance to selective agents. The agar medium in combination with immunomagnetic separation was evaluated and successfully allowed for the detection and isolation of these six serogroups from artificially contaminated fresh beef. The agar medium in combination with immunomagnetic separation also allowed successful detection and isolation of naturally occurring non-O157 STEC strains present in cattle feces. Thirty-five strains of the top six non-O157 STEC serogroups were isolated from 1,897 fecal samples collected from 271 feedlot cattle. This chromogenic agar medium could help significantly in routine screening for the top six non-O157 STEC serogroups from beef cattle and other food. C1 [Kalchayanand, Norasak; Arthur, Terrance M.; Bosilevac, Joseph M.; Wells, James E.; Wheeler, Tommy L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Kalchayanand, N (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM norasak.kalchayanand@ars.usda.gov NR 33 TC 21 Z9 23 U1 1 U2 20 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2013 VL 76 IS 2 BP 192 EP 199 DI 10.4315/0362-028X.JFP-12-182 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240EP UT WOS:000326078000001 PM 23433363 ER PT J AU Kalchayanand, N Arthur, TM Bosilevac, JM Brichta-Harhay, DM Shackelford, SD Wells, JE Wheeler, TL Koohmaraie, M AF Kalchayanand, Norasak Arthur, Terrance M. Bosilevac, Joseph M. Brichta-Harhay, Dayna M. Shackelford, Steven D. Wells, James E. Wheeler, Tommy L. Koohmaraie, Mohammad TI Isolation and Characterization of Clostridium difficile Associated with Beef Cattle and Commercially Produced Ground Beef SO JOURNAL OF FOOD PROTECTION LA English DT Article ID BINARY TOXIN; ADP-RIBOSYLTRANSFERASE; PROCESSING PLANTS; SELECTIVE MEDIUM; RETAIL MEAT; B GENES; STRAINS; PREVALENCE; TOXINOTYPES; DIARRHEA AB The incidence of Clostridium difficile infection has recently increased in North American and European countries. This pathogen has been isolated from retail pork, turkey, and beef products and reported associated with human illness. This increase in infections has been attributed to the emergence of a toxigenic strain designated North America pulsed-field gel electrophoresis type 1 (NAP1). The NAP1 strain has been isolated from calves as well as ground meat products, leading to speculation of illness from consumption of contaminated meat products. However, information on C. difficile associated with beef cattle during processing and commercially produced ground beef is limited. To address this data gap, samples from various steps during beef production were collected. Samples from hides (n = 525), preevisceration carcasses (n = 475), postintervention carcasses (n = 471), and 956 commercial ground beef samples were collected from across the United States. The prevalence of C. difficile spores on hides was 3.2%. C. difficile spores were not detected on preevisceration and postintervention carcasses or in commercially produced ground beef. Phenotypic and genetic characterizations were carried out for all 18 isolates collected from hide samples. Twenty-two percent of the isolates were nontoxigenic strains, while 78% of the isolates were toxigenic. Toxinotyping and PCR ribotyping patterns revealed that 6 and 33% of the isolates were identified as NAP1 and NAP7 strains, respectively. This article evidences that the prevalence of C. difficile, specifically pathogenic strains, in the U. S. beef production chain is low. C1 [Kalchayanand, Norasak; Arthur, Terrance M.; Bosilevac, Joseph M.; Brichta-Harhay, Dayna M.; Shackelford, Steven D.; Wells, James E.; Wheeler, Tommy L.; Koohmaraie, Mohammad] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Kalchayanand, N (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM norasak.kalchayanand@ars.usda.gov NR 54 TC 2 Z9 2 U1 0 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2013 VL 76 IS 2 BP 256 EP 264 DI 10.4315/0362-028X.JFP-12-261 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240EP UT WOS:000326078000010 PM 23433373 ER PT J AU Maitland, J Boyer, R Gallagher, D Duncan, S Bauer, N Kause, J Eifert, J AF Maitland, Jessica Boyer, Renee Gallagher, Dan Duncan, Susan Bauer, Nate Kause, Janell Eifert, Joseph TI Tracking Cross-Contamination Transfer Dynamics at a Mock Retail Deli Market Using GloGerm SO JOURNAL OF FOOD PROTECTION LA English DT Article ID LISTERIA-MONOCYTOGENES CONTAMINATION; POULTRY-PROCESSING PLANTS; STAINLESS-STEEL; MODELING TRANSFER; RISK-ASSESSMENT; TURKEY BREAST; MEAT; BOLOGNA; ENVIRONMENTS; PREVALENCE AB Ready-to-eat (RTE) deli meats are considered a food at high risk for causing foodborne illness. Deli meats are listed as the highest risk RTE food vehicle for Listeria monocytogenes. Cross-contamination in the retail deli market may contribute to spread of pathogens to deli meats. Understanding potential cross-contamination pathways is essential for reducing the risk of contaminating various products. The objective of this study was to track cross-contamination pathways through a mock retail deli market using an abiotic surrogate, GloGerm, to visually represent how pathogens may spread through the deli environment via direct contact with food surfaces. Six contamination origination sites (slicer blade, meat chub, floor drain, preparation table, employee's glove, and employee's hands) were evaluated separately. Each site was inoculated with 20 ml of GloGerm, and a series of standard deli operations were completed (approximately 10 min of work). Photographs were then taken under UV illumination to visualize spread of GloGerm throughout the deli. A sensory panel evaluated the levels of contamination on the resulting contaminated surfaces. Five of the six contamination origination sites were associated with transfer of GloGerm to the deli case door handle, slicer blade, meat chub, preparation table, and the employee's gloves. Additional locations became contaminated (i.e., deli case shelf, prep table sink, and glove box), but this contamination was not consistent across all trials. Contamination did not spread from the floor drain to any food contact surfaces. The findings of this study reinforce the need for consistent equipment cleaning and food safety practices among deli workers to minimize cross-contamination. C1 [Maitland, Jessica; Boyer, Renee; Duncan, Susan; Eifert, Joseph] Virginia Polytech Inst & State Univ, Dept Food Sci & Technol, Blacksburg, VA 24061 USA. [Gallagher, Dan] Virginia Polytech Inst & State Univ, Dept Civil & Environm Engn, Blacksburg, VA 24061 USA. [Bauer, Nate; Kause, Janell] US Food Safety & Inspect Serv, USDA, Off Publ Hlth Sci, Risk Assessment Div, Washington, DC 20250 USA. RP Boyer, R (reprint author), Virginia Polytech Inst & State Univ, Dept Food Sci & Technol, Blacksburg, VA 24061 USA. EM rrboyer@vt.edu FU U.S. Department of Agriculture, Food Safety and Inspection Service, Office of Public Health Science (FSIS) [AG-3A94-C-10-007] FX This research was funded by the U.S. Department of Agriculture, Food Safety and Inspection Service, Office of Public Health Science (FSIS contract AG-3A94-C-10-007). NR 50 TC 5 Z9 5 U1 2 U2 10 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2013 VL 76 IS 2 BP 272 EP 282 DI 10.4315/0362-028X.JFP-12-271 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240EP UT WOS:000326078000012 PM 23433375 ER PT J AU Ding, T Wang, J Park, MS Hwang, CA Oh, DH AF Ding, Tian Wang, Jun Park, Myoung-Su Hwang, Cheng-An Oh, Deog-Hwan TI A Probability Model for Enterotoxin Production of Bacillus cereus as a Function of pH and Temperature SO JOURNAL OF FOOD PROTECTION LA English DT Article ID LACTIC-ACID; GROWTH LIMITS; TOXIN; STRAINS AB Bacillus cereus is frequently isolated from a variety of foods, including vegetables, dairy products, meats, and other raw and processed foods. The bacterium is capable of producing an enterotoxin and emetic toxin that can cause severe nausea, vomiting, and diarrhea. The objectives of this study were to assess and model the probability of enterotoxin production of B. cereus in a broth model as affected by the broth pH and storage temperature. A three-strain mixture of B. cereus was inoculated in tryptic soy broth adjusted to pH 5.0, 6.0, 7.2, 8.0, and 8.5, and the samples were stored at 15, 20, 25, 30, and 35 degrees C for 24 h. A total of 25 combinations of pH and temperature, each with 10 samples, were tested. The presence of enterotoxin in broth was assayed using a commercial test kit. The probabilities of positive enterotoxin production in 25 treatments were fitted with a logistic regression to develop a probability model to describe the probability of toxin production as a function of pH and temperature. The resulting model showed that the probabilities of enterotoxin production of B. cereus in broth increased as the temperature increased and/or as the broth pH approached 7.0. The model described the experimental data satisfactorily and identified the boundary of pH and temperature for the production of enterotoxin. The model could provide information for assessing the food poisoning risk associated with enterotoxins of B. cereus and for the selection of product pH and storage temperature for foods to reduce the hazards associated with B. cereus. C1 [Ding, Tian] Zhejiang Univ, Dept Food Sci & Nutr, Sch Biosyst Engn & Food Sci, Hangzhou 310058, Zhejiang, Peoples R China. [Ding, Tian; Wang, Jun; Park, Myoung-Su; Oh, Deog-Hwan] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, Gangwon, South Korea. [Ding, Tian; Wang, Jun; Park, Myoung-Su; Oh, Deog-Hwan] Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon 200701, Gangwon, South Korea. [Hwang, Cheng-An] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Oh, DH (reprint author), Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, Gangwon, South Korea. EM deoghwa@kangwon.ac.kr RI Wang, Jun/H-4803-2011 OI Wang, Jun/0000-0001-7676-0493 NR 26 TC 3 Z9 3 U1 0 U2 11 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2013 VL 76 IS 2 BP 343 EP 347 DI 10.4315/0362-028X.JFP-12-174 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240EP UT WOS:000326078000023 PM 23433386 ER PT J AU Zhang, DS Yang, Q Zhu, JY Pan, XJ AF Zhang, D. S. Yang, Q. Zhu, J. Y. Pan, X. J. TI Sulfite (SPORL) pretreatment of switchgrass for enzymatic saccharification SO BIORESOURCE TECHNOLOGY LA English DT Article DE Switchgrass; SPORL pretreatment; Dilute acid pretreatment; Alkali pretreatment; Enzymatic saccharification ID CELLULOSIC ETHANOL-PRODUCTION; DILUTE-ACID PRETREATMENTS; LODGEPOLE PINE; AQUEOUS AMMONIA; FERMENTATION; BIOMASS; ENERGY; HYDROLYSIS; SOAKING; LIGNIN AB SPORL (Sulfite Pretreatment to Overcome Recalcitrance of Lignocellulose) pretreatment was applied to switchgrass and optimized through an experimental design using Response Surface Methodology within the range of temperature (163-197 degrees C), time (3-37 min), sulfuric acid dosage (0.8-4.2% on switchgrass), and sodium sulfite dosage (0.6-7.4% on switchgrass). Performance of SPORL was compared with that of dilute acid (DA) and alkali (AL) in switchgrass pretreatment. Results indicated that SPORL pretreatment improved the digestibility of switchgrass through sufficiently removing hemicellulose, partially dissolving lignin, and reducing hydrophobicity of lignin by sulfonation. The removal of hemicellulose was more critical to substrate digestibility than the removal of lignin during SPORL pretreatment. SPORL pretreated switchgrass had better enzymatic digestibility than DA and AL pretreated ones. The SPORL pretreated switchgrass could be hydrolyzed by 83% within 48 h with 15 FPU (filter paper unit) cellulase and 30 CBU (cellobiose unit) beta-glucosidase/g cellulose. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Zhang, D. S.; Yang, Q.; Zhu, J. Y.; Pan, X. J.] Univ Wisconsin, Madison, WI 53706 USA. [Zhu, J. Y.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Pan, XJ (reprint author), Univ Wisconsin, 460 Henry Mall, Madison, WI 53706 USA. EM xpan@wisc.edu FU USDA Forest Service Program Woody Biomass, Bioenergy, and Bioproducts; NSF [CBET-0847049] FX The authors thank Dr. Kevin Shinners for generously providing the switchgrass and Dr. Nicholas Keuler for his kind help with statistic analysis. This research was supported by a grant from USDA Forest Service Program Woody Biomass, Bioenergy, and Bioproducts (2008) to J.Y. Zhu and X.J. Pan and in part by a NSF Career Award (CBET-0847049) to X.J. Pan. NR 37 TC 23 Z9 26 U1 5 U2 42 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2013 VL 129 BP 127 EP 134 DI 10.1016/j.biortech.2012.11.031 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 220EU UT WOS:000324566000018 PM 23232228 ER PT J AU Dubey, JP Darrington, C Tiao, N Ferreira, LR Choudhary, S Molla, B Saville, WJA Tilahun, G Kwok, OCH Gebreyes, WA AF Dubey, J. P. Darrington, C. Tiao, N. Ferreira, L. R. Choudhary, S. Molla, B. Saville, W. J. A. Tilahun, G. Kwok, O. C. H. Gebreyes, W. A. TI ISOLATION OF VIABLE TOXOPLASMA GONDII FROM TISSUES AND FECES OF CATS FROM ADDIS ABABA, ETHIOPIA SO JOURNAL OF PARASITOLOGY LA English DT Article AB Cats are important in the epidemiology of Toxoplasma gondii because they are the only hosts that excrete environmentally resistant oocysts in feces. In the present study, hearts, serum, and feces from 36 feral cats from Addis Ababa area, Ethiopia, were examined for T. gondii infection. Antibodies to T. gondii were determined with the modified agglutination test (MAT, cutoff 1:25); 33 cats were seropositive. Hearts of all 36 cats were homogenized, digested in pepsin, and bioassayed in mice. Feces were examined for T. gondii oocysts by bioassay in mice. Viable T. gondii was isolated from heart of 26 by bioassay in mice and from 25 seropositive and 1 seronegative cats. Toxoplasma gondii was isolated from feces (oocysts) by bioassay in mice. In total, viable T. gondii was isolated from 27 of the 36 cats, and these isolates were designated TgCatEt1 to TgCatEt27. The high prevalence of T. gondii oocysts in feces of 8 (19.4%) of 36 cats is of high epidemiologic significance. This is the first report of isolation of viable T. gondii from any host in Ethiopia. C1 [Dubey, J. P.; Darrington, C.; Tiao, N.; Ferreira, L. R.; Choudhary, S.; Molla, B.; Saville, W. J. A.; Tilahun, G.; Kwok, O. C. H.; Gebreyes, W. A.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 9 TC 11 Z9 11 U1 0 U2 4 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD FEB PY 2013 VL 99 IS 1 BP 56 EP 58 DI 10.1645/GE-3229.1 PG 3 WC Parasitology SC Parasitology GA 192XC UT WOS:000322520000011 PM 22924928 ER PT J AU Dubey, JP Prowell, M AF Dubey, J. P. Prowell, M. TI Ante-Mortem Diagnosis, Diarrhea, Oocyst Shedding, Treatment, Isolation, and Genetic Typing of Toxoplasma gondii Associated with Clinical Toxoplasmosis in a Naturally Infected Cat SO JOURNAL OF PARASITOLOGY LA English DT Article ID MYELITIS AB Toxoplasma gondii infections are common in humans and other animals, but clinical disease is relatively rare. It is unknown whether the severity of toxoplasmosis in immunocompetent hosts is due to the parasite strain, host variability, or to other factors. Recently, attention has been focused on the genetic variability among T. gondii isolates from apparently healthy and sick hosts. Whether T. gondii genetic makeup plays a part in the pathogenesis of clinical feline toxoplasmosis is uncertain because little is known of genetic typing of strains associated with clinical feline toxoplasmosis. A 6-mo-old domestic male cat was hospitalized because of lethargy, anorexia, fever, and diarrhea. Numerous (6 million in 1 sample) T. gondii oocysts were found in feces of the cat and antibodies to T. gondii (titer 1:800) were found in its serum by the modified agglutination test. The cat was medicated orally with Clindamycin for 10 days; it became asymptomatic after 10 days and was discharged from the hospital. Viable T. gondii (designated TgCatUs9) was isolated from feces (oocysts) by bioassays in mice. Genetic typing using the DNA extracted from the brains of infected mice and 10 PCR-restriction fragment length polymorphism (RFLP) markers revealed Type II allele at the SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, and PK1 loci and Type I at the L358 and Apico loci; therefore, this isolate belongs to the ToxoDB PCR-RFLP genotype no. 4, which is grouped into the Type 12 lineage that is dominant in wildlife from North America. To our knowledge, this is the first T. gondii isolate characterized genetically from a sick cat in the USA. C1 [Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Prowell, M.] Beltsville Vet Hosp, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 21 TC 7 Z9 8 U1 1 U2 40 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD FEB PY 2013 VL 99 IS 1 BP 158 EP 160 DI 10.1645/GE-3257.1 PG 3 WC Parasitology SC Parasitology GA 192XC UT WOS:000322520000028 PM 22924914 ER PT J AU Rosypal, AC Bowman, SS Epps, SA El Behairy, AM Hilali, M Dubey, JP AF Rosypal, Alexa C. Bowman, Shanae S. Epps, Samuel A. El Behairy, A. M. Hilali, M. Dubey, J. P. TI Serological Survey of Dogs From Egypt for Antibodies to Leishmania Species SO JOURNAL OF PARASITOLOGY LA English DT Article ID VISCERAL LEISHMANIASIS; DOMESTIC DOGS; EASTERN SUDAN; HUMANS; HOST AB Leishmaniasis is an insect-transmitted parasitic disease with a worldwide distribution. Leishmania spp. infections cause a broad spectrum of clinical signs, ranging from skin lesions to fatal visceral disease. Dogs are a major reservoir host for visceral leishmaniasis in humans. While the disease is endemic in the Middle East and North Africa, little is known concerning canine Leishmania spp. infections in Egypt. Accordingly, blood samples were collected from 50 stray dogs in Giza, Egypt. Canine sera were tested for antibodies to visceralizing Leishmania spp. by commercial immunochromatographic strip assays based on recombinant antigen K39. Antibodies to Leishmania spp. were found in 5 of 50 (10%) of dogs tested from Egypt. Results from this study indicate that stray dogs are exposed to visceralizing Leishmania species in Egypt. C1 [Rosypal, Alexa C.; Bowman, Shanae S.; Epps, Samuel A.] Johnson C Smith Univ, Dept Nat Sci & Math, Coll Sci Technol Engn & Math, Charlotte, NC 28216 USA. [El Behairy, A. M.; Hilali, M.] Cairo Univ, Dept Parasitol, Fac Vet Med, Giza, Egypt. [Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Rosypal, AC (reprint author), Johnson C Smith Univ, Dept Nat Sci & Math, Coll Sci Technol Engn & Math, Charlotte, NC 28216 USA. EM jitender.dubey@ars.usda.gov RI Rosypal, Alexa/I-7114-2016; Hilali, Mosaad/O-9443-2016; OI A. Hilali, Mosaad/0000-0002-9930-8501 FU Smith Institute of Applied Research at Johnson C. Smith University FX This work was supported in part by a mini-grant from the Smith Institute of Applied Research at Johnson C. Smith University to ACR. NR 16 TC 3 Z9 3 U1 0 U2 4 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD FEB PY 2013 VL 99 IS 1 BP 170 EP 171 DI 10.1645/GE-3242.1 PG 2 WC Parasitology SC Parasitology GA 192XC UT WOS:000322520000032 PM 22924913 ER PT J AU Picklo, MJ Idso, JP Jackson, MI AF Picklo, Matthew J., Sr. Idso, Joseph P. Jackson, Matthew I. TI S-Glutathionylation of Hepatic and Visceral Adipose Proteins Decreases in Obese Rats SO OBESITY LA English DT Article ID DIET-INDUCED OBESITY; FACTOR-KAPPA-B; OXIDATIVE STRESS; TYROSINE PHOSPHATASES; REVERSIBLE OXIDATION; INSULIN SENSITIVITY; REDOX REGULATION; SULFENIC ACID; MODEL; MICE AB A number of clinical and biochemical studies demonstrate that obesity and insulin resistance are associated with increases in oxidative stress and inflammation. Paradoxically, insulin sensitivity can be enhanced by oxidative inactivation of cysteine residues of phosphatases, and inflammation can be reduced by S-glutathionylation with formation of protein-glutathione mixed disulfides (PSSG). Although oxidation of protein-bound thiols (PSH) is increased in multiple diseases, it is not known whether there are changes in PSH oxidation species in obesity. Objective: In this work, the hypothesis that obesity is associated with decreased levels of proteins containing oxidized protein thiols was tested. Design and Methods: The tissue levels of protein sulfenic acids (PSOH) and PSSG in liver, visceral adipose tissue, and skeletal muscle derived from glucose intolerant, obese-prone Sprague-Dawley rats were examined. Results: The data in this study indicate that decreases in PSSG content occurred in liver (44%) and adipose (26%) but not skeletal muscle in obese rats that were fed a 45% fat-calorie diet versus lean rats that were fed a 10% fat-calorie diet. PSOH content did not change in the tissue between the two groups. The activity of the enzyme glutaredoxin (GLRX) responsible for reversal of PSSG formation did not change in muscle and liver between the two groups. However, levels of GLRX1 were elevated 70% in the adipose tissue of the obese, 45% fat calorie-fed rats. Conclusion: These are the first data to link changes in S-glutathionylation and GLRX1 to adipose tissue in the obese and demonstrate that redox changes in thiol status occur in adipose tissue as a result of obesity. C1 [Picklo, Matthew J., Sr.; Idso, Joseph P.; Jackson, Matthew I.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Picklo, MJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM matthew.picklo@ars.usda.gov FU USDA [5450-51000-048-00D] FX This work was funded by USDA 5450-51000-048-00D. NR 40 TC 8 Z9 8 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 J9 OBESITY JI Obesity PD FEB PY 2013 VL 21 IS 2 BP 297 EP 305 DI 10.1002/oby.20002 PG 9 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 187AH UT WOS:000322087300013 PM 23404913 ER PT J AU Davis, JP Sweigart, DS Price, KM Dean, LL Sanders, TH AF Davis, Jack P. Sweigart, Daniel S. Price, Kristin M. Dean, Lisa L. Sanders, Timothy H. TI Refractive Index and Density Measurements of Peanut Oil for Determining Oleic and Linoleic Acid Contents SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Density; Refractive index; Peanut; Peanut oil; High oleic; O/L ratio ID VEGETABLE-OILS; FATTY-ACIDS; STABILITY AB Peanut seed are approximately 50 % oil of which [80 % is either oleic or linoleic acid. The oleic/linoleic acid (O/L) ratio largely influences oxidative stability and hence peanut shelf life. Traditional peanut seed have O/L ratios near 1.5-2.0; however, many new cultivars are "high oleic" with O/L ratios >= 9. During peanut seed handling, contamination among lots may occur. A cost effective method to rapidly differentiate peanut seed based on O/L ratio is needed across multiple segments of the industry, and measurements of oil density and oil refractive index (RI) were evaluated for this potential. Fatty acid profiles of samples from normal and high oleic seed lots, and blends of these oils, were determined by traditional gas chromatography analysis and this data compared to corresponding oil density and RI measurements. Oleic acid content, linoleic acid content, density and RI were all strongly linearly (R-2 > 0.98) correlated for oil blends with O/L ratios from similar to 2 to 16. Threshold density or RI values both showed excellent potential for rapidly differentiating samples with an O/L >= 9; however, sample volume requirements preclude density measurements on single seed. C1 [Davis, Jack P.; Dean, Lisa L.; Sanders, Timothy H.] N Carolina State Univ, ARS, USDA, Market Qual & Handling Res Unit,Dept Food Bioproc, Raleigh, NC 27695 USA. [Sweigart, Daniel S.] Hershey Co, Ctr Tech, Hershey, PA 17033 USA. [Price, Kristin M.] ARS, USDA, Market Qual & Handling Res Unit, Raleigh, NC 27695 USA. RP Davis, JP (reprint author), N Carolina State Univ, ARS, USDA, Market Qual & Handling Res Unit,Dept Food Bioproc, 236C Schaub Hall, Raleigh, NC 27695 USA. EM jack.davis@ars.usda.gov RI Dean, Lisa/B-1463-2015 OI Dean, Lisa/0000-0002-2407-9548 NR 20 TC 2 Z9 3 U1 0 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2013 VL 90 IS 2 BP 199 EP 206 DI 10.1007/s11746-012-2153-4 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 164TL UT WOS:000320432500004 ER PT J AU Bilbao-Sainz, C Chiou, BS Du, WX Gregorsky, KS Orts, WJ AF Bilbao-Sainz, Cristina Chiou, Bor-Sen Du, Wen-Xian Gregorsky, Kay S. Orts, William J. TI Influence of Disperse Phase Characteristics on Stability, Physical and Antimicrobial Properties of Emulsions Containing Cinnamaldehyde SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Emulsion; Droplet size; Color; Viscosity; Antimicrobial; Cinnamaldehyde ID IN-WATER EMULSIONS; ESSENTIAL OILS; DROPLET SIZE; LISTERIA-MONOCYTOGENES; SALMONELLA-ENTERICA; ESCHERICHIA-COLI; RHEOLOGY AB Cinnamaldehyde was delivered in emulsion form using Acetem 90-50K as a carrier and Tween 60 as emulsifier. Cinnamaldehyde interacted with Acetem 90-50K by forming H-bonds. The effect of disperse phase characteristics on storage stability, physical and antimicrobial properties was investigated. A storage test of emulsions was carried out for 15 days at two temperatures (22 and 4 degrees C). Emulsions and nano-emulsions showed higher stability at 22 degrees C than at 4 degrees C. Nano-emulsions displayed excellent stability versus creaming and coalescence after 15 days storage at 22 degrees C (z-avg <100 nm). Physical properties were greatly affected by droplet size and concentration. Emulsions became less viscous, more transparent and darker as the droplet size or concentration decreased. The antimicrobial activity was measured against Listeria monocytogenes and Escherichia coli. Escherichia coli was highly resistant to cinnamaldehyde compared to L. monocytogenes. Incubation with cinnamaldehyde at 2.5 mM caused the complete inactivation of L. monocytogenes after 1 day and of E. coli after 9 days. There was no difference in the antimicrobial effect of cinnamaldehyde due to different droplet sizes (similar to 80 and similar to 5,000 nm). C1 [Bilbao-Sainz, Cristina; Chiou, Bor-Sen; Du, Wen-Xian; Gregorsky, Kay S.; Orts, William J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Bilbao-Sainz, C (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM cristina.bilbao@ars.usda.gov NR 21 TC 5 Z9 5 U1 5 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2013 VL 90 IS 2 BP 233 EP 241 DI 10.1007/s11746-012-2164-1 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 164TL UT WOS:000320432500008 ER PT J AU Sales, MA Murphy, KY Reiter, ST Brown, AH Brown, MA Looper, ML Rosenkrans, CF AF Sales, M. A. Murphy, K. Y. Reiter, S. T. Brown, A. H., Jr. Brown, M. A. Looper, M. L. Rosenkrans, C. F., Jr. TI Effects of forage type, body condition and single-nucleotide polymorphisms in the bovine cytochrome P450 regulatory region on cow productivity SO JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION LA English DT Article DE body condition; cattle; cytochrome P450; gene promoter; tall fescue ID ERGOT ALKALOIDS; BRAHMAN; MILK; FESCUE; ANGUS; PERFORMANCE; METABOLISM; CALVES AB Single-nucleotide polymorphisms (SNP) in the coding sequence of cytochrome p450 (CYP3A28) have been associated with milk yield and composition, and calving traits in cows. In this study, we aimed to determine whether (i) the CYP3A28 regulatory region was polymorphic and (ii) SNP genotype, forage type, body condition and their interactions affect cow productivity. Primers for CYP3A28 promoter were designed to amplify a 483-bp segment by PCR. Amplicon sequences revealed seven SNP (T-318C, T-113A, C-189T, T-78G, A6G, G17A and T21C) in Brahman (38 cows), Brahman x Angus reciprocal crosses (47 cows) and crossbreds (98 cows). Angus cows (n = 41) appeared to be fixed at those SNP locations. Genotype and forage {endophyte-infected tall fescue [KY+; Lolium arundinaceum (Schreb.) S. J. Darbyshire] vs. bermudagrass [Cynodon dactylon (L.) Pers.]} effects on lifetime (8-years) calving rate, and calf weaning weights and heights were determined in Herd 1 (126 cows); genotype and BC (low vs. moderate) effects on calving date and calving percent were determined in Herd 2 (98 cows). Four SNP (T-318C, T-113A, A06G and T21C) appeared to be related to cattle productivity, CC cows at T-318C having a lower (p < 0.05) lifetime calving rate than TC or TT cows (65%, 85% and 81% respectively). Cows that grazed KY+ and were TT at T-318C produced calves that tended (p < 0.07) to weigh less than their contemporaries. Moreover, calves of TT cows were shorter (p < 0.05) at weaning than calves of CC or TC cows. In Herd 2, moderate-BC cows that were TT or AA at T-318C, T-113A, T-78G, A6G and T21C had greater (p < 0.05) calving rates (7480%) than heterozygous cows (4660%), and low-BC cows that were AA at G17A calved at least 6 days earlier (p < 0.05) than heterozygous cows. Our findings suggest that SNP in the CYP3A28 regulatory region of Brahman-influenced cows are associated with cattle productivity. C1 [Sales, M. A.; Murphy, K. Y.; Reiter, S. T.; Brown, A. H., Jr.; Rosenkrans, C. F., Jr.] Univ Arkansas, Dept Anim Sci, Fayetteville, AR 72701 USA. [Brown, M. A.] USDA ARS, El Reno, OK USA. [Looper, M. L.] USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR USA. RP Rosenkrans, CF (reprint author), 1 Univ Arkansas, Dept Anim Sci, AFLS B107-E, Fayetteville, AR 72701 USA. EM crosenkr@uark.edu FU U.S. Department of Agriculture [58-6227-8-040]; Student Undergraduate Research Fellowship grant FX This work was supported in part by the U.S. Department of Agriculture, under specific cooperative agreement No. 58-6227-8-040, as well as a Student Undergraduate Research Fellowship grant. We thank Bobbie Okimoto, University of Arkansas DNA Resource Center, for technical assistance in gene sequencing. NR 17 TC 1 Z9 1 U1 0 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2439 J9 J ANIM PHYSIOL AN N JI J. Anim. Physiol. Anim. Nutr. PD FEB PY 2013 VL 97 IS 1 BP 91 EP 96 DI 10.1111/j.1439-0396.2011.01246.x PG 6 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 077YS UT WOS:000314065900011 PM 22054297 ER PT J AU Cole, JB Lewis, RM Maltecca, C Newman, S Olson, KM Tait, RG AF Cole, J. B. Lewis, R. M. Maltecca, C. Newman, S. Olson, K. M. Tait, R. G., Jr. TI BREEDING AND GENETICS SYMPOSIUM: Systems biology in animal breeding: Identifying relationships among markers, genes, and phenotypes SO JOURNAL OF ANIMAL SCIENCE LA English DT Editorial Material C1 [Cole, J. B.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [Lewis, R. M.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Maltecca, C.] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Newman, S.] Genus Plc, Hendersonville, TN 37075 USA. [Olson, K. M.] ABS Global Inc, De Forest, WI 53532 USA. [Tait, R. G., Jr.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Cole, JB (reprint author), USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. EM john.cole@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Tait, Jr., Richard/0000-0002-2379-7740 NR 8 TC 1 Z9 1 U1 1 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 521 EP 522 DI 10.2527/jas.2012-6166 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800001 PM 23348684 ER PT J AU Bennett, GL Shackelford, SD Wheeler, TL King, DA Casas, E Smith, TPL AF Bennett, G. L. Shackelford, S. D. Wheeler, T. L. King, D. A. Casas, E. Smith, T. P. L. TI Selection for genetic markers in beef cattle reveals complex associations of thyroglobulin and casein1-S1 with carcass and meat traits SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT BREEDING AND GENETICS SYMPOSIUM CY JUL 15-19, 2012 CL Phoenix, AZ DE casein; cattle; epistasis; genotype; marker association; thyroglobulin ID SINGLE NUCLEOTIDE POLYMORPHISMS; INFRARED REFLECTANCE SPECTROSCOPY; LONGISSIMUS TENDERNESS; ONLINE CLASSIFICATION; INTRAMUSCULAR FAT; POSTNATAL-GROWTH; POPULATIONS; EPISTASIS; HETEROSIS; SYSTEM AB Genetic markers in casein (CSN1S1) and thyroglobulin (TG) genes have previously been associated with fat distribution in cattle. Determining the nature of these genetic associations (additive, recessive, or dominant) has been difficult, because both markers have small minor allele frequencies in most beef cattle populations. This results in few animals homozygous for the minor alleles. Selection to increase the frequencies of the minor alleles for 2 SNP markers in these genes was undertaken in a composite population. The objective was to obtain better estimates of genetic effects associated with these markers and determine if there were epistatic interactions. Selection increased the frequencies of minor alleles for both SNP from <0.30 to 0.45. Bulls (n = 24) heterozygous for both SNP were used in 3 yr to produce 204 steer progeny harvested at an average age of 474 d. The combined effect of the 9 CSN1S1 x TG genotypes was associated with carcass-adjusted fat thickness (P < 0.06) and meat tenderness predicted at the abattoir by visible and near-infrared reflectance spectroscopy (P < 0.04). Genotype did not affect BW from birth through harvest, ribeye area, marbling score, slice shear force, or imagebased yield grade (P > 0.10). Additive, dominance, and epistatic SNP association effects were estimated from genotypic effects for adjusted fat thickness and predicted meat tenderness. Adjusted fat thickness showed a dominance association with TG SNP (P < 0.06) and an epistatic additive CSN1S1 x additive TG association (P < 0.03). For predicted meat tenderness, heterozygous TG meat was more tender than meat from either homozygote (P < 0.002). Dominance and epistatic associations can result in different SNP allele substitution effects in populations where SNP have the same linkage disequilibrium with causal mutations but have different frequencies. Although the complex associations estimated in this study would contribute little to within-population selection response, they could be important for marker-assisted management or reciprocal selection schemes. C1 [Bennett, G. L.; Shackelford, S. D.; Wheeler, T. L.; King, D. A.; Casas, E.; Smith, T. P. L.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Bennett, GL (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM gary.bennett@ars.usda.gov NR 30 TC 6 Z9 6 U1 1 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 565 EP 571 DI 10.2527/jas.2012-5454 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800006 PM 23148258 ER PT J AU Daniels, KM Farmer, C Jimenez-Flores, R Rijnkels, M AF Daniels, K. M. Farmer, C. Jimenez-Flores, R. Rijnkels, M. TI The long-term impact of epigenetics and maternal influence on the neonate through milk-borne factors and nutrient status SO JOURNAL OF ANIMAL SCIENCE LA English DT Editorial Material ID TEMPERAMENT; EXPRESSION; MOTHERS C1 [Daniels, K. M.] Ohio State Univ, Dept Anim Sci, OARDC, Wooster, OH 44691 USA. [Farmer, C.] Agr & Agri Food Canada, Dairy & Swine R&D Ctr, Sherbrooke, PQ J1M 0C8, Canada. [Jimenez-Flores, R.] Calif Polytech State Univ San Luis Obispo, Dept Dairy Sci, San Luis Obispo, CA 93407 USA. [Rijnkels, M.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Daniels, KM (reprint author), Ohio State Univ, Dept Anim Sci, OARDC, Wooster, OH 44691 USA. EM daniels.412@osu.edu RI Jimenez-Flores, Rafael/I-5229-2013; OI Daniels, Kristy/0000-0002-1437-1457 NR 14 TC 0 Z9 0 U1 0 U2 16 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 673 EP 675 DI 10.2527/jas.2013-6237 PG 3 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800017 PM 23482304 ER PT J AU Bartol, FF Wiley, AA Miller, DJ Silva, AJ Roberts, KE Davolt, MLP Chen, JC Frankshun, AL Camp, ME Rahman, KM Vallet, JL Bagnell, CA AF Bartol, F. F. Wiley, A. A. Miller, D. J. Silva, A. J. Roberts, K. E. Davolt, M. L. P. Chen, J. C. Frankshun, A-L Camp, M. E. Rahman, K. M. Vallet, J. L. Bagnell, C. A. TI LACTATION BIOLOGY SYMPOSIUM: Lactocrine signaling and developmental programming SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE colostrum; development; female reproductive tract; lactocrine; neonate ID PORCINE ENDOMETRIAL DEVELOPMENT; ENDOCRINE-DISRUPTING CHEMICALS; ENDOTHELIAL GROWTH-FACTOR; MILK-BORNE RELAXIN; RECEPTOR EXPRESSION; UTERINE DEVELOPMENT; GENE-EXPRESSION; NEONATAL PIGS; DNA-SYNTHESIS; HUMAN-DISEASE AB Lactocrine signaling is defined as transmission of bioactive factors from mother to offspring as a consequence of nursing. Lactocrine transmission of signaling molecules may be an evolutionarily conserved process through which bioactive factors necessary for support of neonatal development are delivered postnatally. Dependence on maternal resources for development in eutherian mammals extends into neonatal life for at least that period of time when nutrition is obtained solely from first milk (i.e., colostrum). Data for the pig (Sus scrofa domesticus) provide evidence of lactocrine mediated effects on development of the female reproductive tract and other somatic tissues. Porcine uterine gland development, an estrogen receptor-alpha (ESR1)-dependent process, begins within 2 d of birth [postnatal day (PND) 0]. A lactocrine-driven, ESR1-mediated process was proposed as a regulatory mechanism governing onset of uterine gland development and endometrial maturation in the neonatal pig. Gilts maintained in a lactocrine-null state for 2 d from birth by milk-replacer feeding displayed altered patterns of endometrial gene expression and retarded uterine gland development by PND 14. In lactocrine-null gilts, inhibition of endometrial and cervical ESR1 and vascular endothelial growth factor (VEGFA) expression observed on PND 2 persisted to PND 14, even after gilts were returned to nursing on PND 2. Collectively, data support a role for lactocrine signaling in regulation of critical neonatal developmental events. Maternal lactocrine programming of postnatal development may help to insure healthy developmental outcomes. A systems biology approach will be required to define and understand mechanistic dynamics of lactocrine signaling events that may ultimately connect genotype to phenotype and establish the parameters of reproductive potential. C1 [Bartol, F. F.; Wiley, A. A.; Miller, D. J.; Silva, A. J.; Roberts, K. E.; Davolt, M. L. P.] Auburn Univ, Dept Anat Physiol & Pharmacol, Cellular & Mol Biosci Program, Auburn, AL 36849 USA. [Chen, J. C.; Frankshun, A-L; Camp, M. E.; Rahman, K. M.; Bagnell, C. A.] Rutgers State Univ, Dept Anim Sci, Endocrinol & Anim Biosci Program, New Brunswick, NJ 08901 USA. [Vallet, J. L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Bartol, FF (reprint author), Auburn Univ, Dept Anat Physiol & Pharmacol, Cellular & Mol Biosci Program, Auburn, AL 36849 USA. EM bartoff@auburn.edu FU American Society of Animal Science; USDA National Institute of Food and Agriculture [2003-35203-13572, 2007-35203-18098]; [NSF-EPS-0814103]; [1158862] FX Based on a presentation at the Lactation Biology Symposium titled "The long-term impact of epigenetics and maternal influence on the neonate through milk-borne factors and nutrient status" at the Joint Annual Meeting, July 15-19, 2012, Phoenix, AZ, with publication sponsored by the Journal of Animal Science and the American Society of Animal Science.; Work reviewed here was supported by National Research Initiative Competitive Grants 2003-35203-13572 and 2007-35203-18098 (to FFB and CAB) from the USDA National Institute of Food and Agriculture and by NSF-EPS-0814103 and 1158862 (to FFB). The authors thank L. Comerford, K. Mezey, B. Anderson, the staffs of the Auburn University and Rutgers University animal care programs, and a multitude of undergraduate students for their contributions to this work. FFB and CAB contributed equally to this work. NR 100 TC 19 Z9 19 U1 1 U2 22 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 696 EP 705 DI 10.2527/jas.2012-5764 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800020 PM 23100582 ER PT J AU Atkins, JA Smith, MF MacNeil, MD Jinks, EM Abreu, FM Alexander, LJ Geary, TW AF Atkins, J. A. Smith, M. F. MacNeil, M. D. Jinks, E. M. Abreu, F. M. Alexander, L. J. Geary, T. W. TI Pregnancy establishment and maintenance in cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cow; embryo survival; fertilization; pregnancy ID GONADOTROPIN-RELEASING-HORMONE; PREOVULATORY FOLLICLE DIAMETER; LACTATING DAIRY-COWS; IN-VITRO MATURATION; OVULATORY FOLLICLE; EMBRYO-TRANSFER; BEEF HEIFERS; GRANULOSA-CELLS; BOVINE OOCYTES; UTERINE PH AB A single ovulation, reciprocal embryo transfer study was used to investigate effects of oocyte competence and maternal environment on pregnancy establishment and maintenance in beef cows. Estrous cycles were synchronized in suckled beef cows and embryo donors were inseminated on d 0 (n = 810). Cows were classified on d 0 as having a small (<12.5 mm) or large (>= 12.5 mm) ovulatory follicle and randomly chosen as donors or recipients to remove confounding effects of ovulatory follicle size on fertility. Embryos (n = 393) or oocytes (n = 44) were recovered on d 7, and all viable embryos were transferred into recipients (n = 354). All statistical analyses were conducted using the GLM procedure of SAS. Path analysis (with significance set at P < 0.10) was used to examine potential cause-effect relationships among the measured variables. Greater donor cow BW, circulating estradiol concentration at insemination, postpartum interval, and ovulatory follicle size directly increased (P < 0.10) fertilization success. Greater donor cow age was the only factor that directly decreased (P < 0.10) fertilization success. Viability of d-7 embryos was directly inhibited (P < 0.10) by rapid follicular growth rate from d -2 to 0 and heavier BW. Direct beneficial effects to embryo viability were increased serum progesterone concentration on d -2 and ovulatory follicle size. Pregnancy maintenance from d 7 to 27 was enhanced (P < 0.10) by increased serum estradiol concentration on d 0 and progesterone concentration on d 7 in the recipient cow. Increased follicular diameter in the recipient cow on d 0 was detrimental to pregnancy maintenance from d 7 to 27. This manuscript defines the complex interplay and relative contributions of endocrine and physical factors both prior and subsequent to fertilization that influence both oocyte competence and maternal environment and their roles in establishment and maintenance of pregnancy. C1 [Atkins, J. A.; Smith, M. F.; Jinks, E. M.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [MacNeil, M. D.; Abreu, F. M.; Alexander, L. J.; Geary, T. W.] ARS, USDA, Miles City, MT 59301 USA. RP Geary, TW (reprint author), ARS, USDA, Miles City, MT 59301 USA. EM tom.geary@ars.usda.gov FU USDA National Institute of Food and Agriculture [2006-35203-17284]; Pfizer; TEVA Animal Health FX This project was supported by National Research Initiative (Grant no. 2006-35203-17284) from the USDA National Institute of Food and Agriculture, and support from Pfizer and TEVA Animal Health. Mention of a proprietary product does not constitute a guarantee or warranty of the product by USDA or the authors and does not imply its approval to the exclusion of other products that may also be suitable. The USDA-ARS, Northern Plains Area, is an equal opportunity/affirmative action employer. All agency services are available without discrimination. The authors acknowledge the large contribution made by the following individuals to successfully complete this project: Mike Woods, Doug Armstrong, Alan Mason, Tyler Johnson, Benny Bryan, Paige Beardsley, Sue Bellows, Whitney Lott, Crystal Roberts, Kathy Meidinger, Rick Harris, Megan Minten, Sara Hanson, Libby Erickson, and Ky Pohler. The following individuals were instrumental in training staff to conduct various aspects of this project: George Seidel, Zella Brink, Cliff Lamb, Cliff Murphy, and Lee Spate. NR 66 TC 20 Z9 20 U1 2 U2 22 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 722 EP 733 DI 10.2527/jas.2012-5368 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800023 PM 23148248 ER PT J AU Hales, KE Cole, NA MacDonald, JC AF Hales, K. E. Cole, N. A. MacDonald, J. C. TI Effects of increasing concentrations of wet distillers grains with solubles in steam-flaked, corn-based diets on energy metabolism, carbon-nitrogen balance, and methane emissions of cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; distillers grains; methane; steam-flaked corn ID IN-VITRO FERMENTATION; FINISHING BEEF-CATTLE; BY-PRODUCTS; CARCASS CHARACTERISTICS; PROTEIN CONCENTRATIONS; PROCESSING METHOD; PERFORMANCE; SORGHUM; RETENTION; BARLEY AB The use of wet distillers grains with solubles (WDGS) in feedlot diets has increased in the Southern Great Plains as a result of the growing ethanol industry. Nutrient balance and respiration calorimetry research evaluating the use of steam-flaked corn (SFC)-based diets in conjunction with WDGS is limited. Therefore, the effects of increasing concentrations of WDGS in a SFC-based diet on energy metabolism, C, and N balance, and enteric methane (CH4) production was evaluated in Jersey steers fed at 2 times maintenance, using respiration calorimetry chambers. Four treatments were used in two 4 x 4 Latin square designs, using 8 steers. Treatments consisted of: 1) SFC-based diet with 0% WDGS (SFC-0); 2) SFC-based diet with 15% WDGS (SFC-15); 3) SFC-based diet with 30% WDGS (SFC-30); and 4) SFC-based diet with 45% WDGS (SFC-45). Diets were balanced for degradable intake protein (DIP) by adding cottonseed meal to the SFC-0 diet. As a proportion of GE, fecal, urinary, and CH4 energy increased linearly (P < 0.03) as WDGS concentration increased in the diet. In contrast, DE, ME, and retained energy decreased linearly (P < 0.01) as a proportion of GE as WDGS concentration increased. Increasing concentration of WDGS in the diet did not affect (P > 0.78) heat production as a proportion of GE. As a result of greater N intake, total N excretion increased linearly (P < 0.01) with increasing WDGS inclusion in the diet. Fecal C loss and CH4-C respired increased linearly (P < 0.01) when WDGS concentration increased in the diet whereas CO2-C respired decreased (linear, P = 0.05) as WDGS concentration increased. We conclude that CH4 production as a proportion of GE increases linearly (P < 0.01) when WDGS concentration in the diet is increased; however, dietary inclusion of WDGS at up to 45% seems to have no effect (P > 0.78) on heat production as a proportion of GE. The reason for a linear decrease in retained energy as WDGS increased was likely because of increased fecal energy loss associated with feeding WDGS. Total N excretion, fecal C loss, and CH4-C respired increased linearly with increasing concentration of WDGS in the diet. We determined NEg values for WDGS to be 2.02, 1.61, and 1.38 Mcal/kg when included at 15%, 30%, and 45%, respectively, in a SFC-based diet. From these results we conclude that the energy value (NEg) of WDGS in a finishing cattle diet based on SFC must be decreased as the inclusion increases. C1 [Hales, K. E.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Cole, N. A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [MacDonald, J. C.] Texas Agrilife Res, Amarillo, TX 79106 USA. RP Hales, KE (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov NR 31 TC 12 Z9 12 U1 0 U2 17 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2013 VL 91 IS 2 BP 819 EP 828 DI 10.2527/jas.2012-5418 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154PH UT WOS:000319687800033 PM 23148244 ER PT J AU Mo, C Hasegawa, M Lee, K Lim, JG Kim, MS Kang, S Lee, HD Bae, H Kim, DY Cho, BK AF Mo, Changyeun Hasegawa, Masumi Lee, Kangjin Lim, Jong-Guk Kim, Moon S. Kang, Sukwon Lee, Hyun-Dong Bae, Hanhong Kim, Dae-Yong Cho, Byoung-Kwan TI Development of a Non-destructive On-line Pungency Measurement System for Red-Pepper Powder SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE capsaicinoids; food quality; non-destructive measurement; red-pepper powder; spectroscopy ID SOLUBLE SOLIDS; CAPSAICINOIDS; TRANSMITTANCE; REFLECTANCE AB Capsaicinoids are the main components of red pepper that determine the spiciness level of red-pepper powders. Current methods for pungency measurement are mostly dependent on HPLC measurement technique, which is a sample-destructive, labor-intensive, time-consuming, and expensive method. In this research, a nondestructive on-line pungency measurement method for red-pepper powder was developed using a Visible/Near-Infrared spectrometer with the wavelength range of 400-1000 nm. The system was constructed with a charge-couple device (CCD) spectrometer, a reference-measuring unit for calibration, and a sample transfer unit, which conveyed the red-pepper powder during measurements. Typically powder samples with eleven different spiciness levels were produced by controlling the mixture of predetermined non-spicy and spicy red-pepper powder. A total of 33 different samples, spanning 11 spiciness levels in three particle sizes (below 0.425 mm, 0.425 to 0.71 mm and 0.71 to 1.4 mm) were prepared for spectral measurements using the developed pungency measuring system. Partial Least Square Regression (PLSR) models were developed to predict the content of capsaicinoids in red-pepper powder from the visible/NIR spectra and reference values measured by HPLC. The R-v(2) and SEP of the PLSR model for red-pepper powders without regard to particle size were 0.973 and +/- 6.98 mg/100 g, respectively. The best result of the PLSR models (R-2=0.991, SEP=+/- 4.33 mg/100 g) was achieved from the raw spectra of red-pepper powders for particle sizes below 0.425 mm. The obtained results demonstrate the potential of NIRS technique for non-destructive and on-line measurement of capsaicinoids content in red-pepper powder. C1 [Mo, Changyeun; Hasegawa, Masumi; Lee, Kangjin; Lim, Jong-Guk; Kim, Moon S.; Kang, Sukwon; Lee, Hyun-Dong; Bae, Hanhong; Kim, Dae-Yong; Cho, Byoung-Kwan] Kyushu Univ, Dept Agroenvironm Sci, Div Sustainable Bioresource Sci, Lab Wood Sci,Fac Agr, Fukuoka 8128581, Japan. [Mo, Changyeun; Lee, Kangjin; Lim, Jong-Guk; Kang, Sukwon; Lee, Hyun-Dong] Natl Acad Agr Sci, Rural Dev Adm, Suwon 441100, Gyeonggi Do, South Korea. [Kim, Moon S.] ARS, Environm Microbiol & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Bae, Hanhong] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea. [Kim, Dae-Yong; Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Taejon 305764, South Korea. RP Cho, BK (reprint author), Chungnam Natl Univ, Dept Biosyst Machinery Engn, 220 Gung Dong, Taejon 305764, South Korea. EM chobk@cnu.ac.kr RI U-ID, Kyushu/C-5291-2016 FU Rural Development Administration; Republic of Korea [PJ008055]; Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea FX This work was partially supported by a grant from the Next Generation BioGeen 21 Program (No. PJ008055), Rural Development Administration; Republic of Korea. It was also partially supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. NR 17 TC 0 Z9 0 U1 2 U2 10 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD FEB PY 2013 VL 58 IS 1 BP 137 EP 144 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA 155ZD UT WOS:000319788400021 ER PT J AU Mack, LA Felver-Gant, JN Dennis, RL Cheng, HW AF Mack, L. A. Felver-Gant, J. N. Dennis, R. L. Cheng, H. W. TI Genetic variations alter production and behavioral responses following heat stress in 2 strains of laying hens SO POULTRY SCIENCE LA English DT Article DE behavior; heat stress; laying hen; physiology; production ID HIGH ENVIRONMENTAL-TEMPERATURE; HIGH AMBIENT-TEMPERATURE; FOWL GALLUS-DOMESTICUS; EGG QUALITY TRAITS; 4 LAYER LINES; GROUP SELECTION; THYROID-HORMONES; GROWTH-RATE; PHYSIOLOGICAL-RESPONSES; PHENOTYPIC CORRELATIONS AB Genetic differences alter the type and degree of hens' responses and their ability to adapt to a stressor. This study examined the effects of genotypic variations on the productivity and behavior of laying hens following heat stress (HS). Two strains of White Leghorn hens were used: DXL (Dekalb XL), a commercial strain individually selected for egg production and KGB (kind, gentle bird), a strain selected for high group productivity and survivability. Ninety hens (48 DXL and 42 KGB) at 28 wk of age were randomly assigned to either a hot (H: mean = 32.6 degrees C) or control (C: mean = 24.3 degrees C) treatment and housed in pairs by strain for 9 d. Egg production and quality, behavior, body and organ weights, and circulating hormone concentrations were measured. Heat-stressed hens had lower egg production [adjusted (adj) P < 0.001] than their respective controls. Among H-DXL hens, egg weight tended to be reduced at d 1 and was reduced at d 9 (adj P = 0.007), but was reduced only at d 9 among H-KGB hens (adj P = 0.007). Eggshell thickness was also reduced among H hens at d 9 (adj P = 0.007), especially among H-KGB hens (adj P = 0.01). Plasma triiodothyronine concentration was reduced among H-hens (adj P = 0.01), especially among H-DXL hens (adj P = 0.01). Neither temperature nor strain affected the plasma thyroxine and plasma and yolk corticosterone concentrations. Heat-stressed hens spent less time walking (adj P = 0.001) and more time drinking (adj P = 0.007) and resting (adj P = 0.001) than C-hens. The results indicate that although HS reduced production and caused behavioral changes among hens from both strains, the responses differed by genotype. The data provide evidence that genetic selection is a useful strategy for reducing HS response in laying hens. The results provide insights for conducting future studies to develop heat-resistant strains to improve hen well-being, especially under the current commercial conditions. C1 [Mack, L. A.; Felver-Gant, J. N.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Dennis, R. L.; Cheng, H. W.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Cheng, HW (reprint author), Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM hwcheng@purdue.edu RI Mack, Laurie/J-7475-2013 OI Mack, Laurie/0000-0003-0409-8992 NR 65 TC 10 Z9 13 U1 0 U2 15 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 285 EP 294 DI 10.3382/ps.2012-02589 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900001 PM 23300291 ER PT J AU Hester, PY Enneking, SA Jefferson-Moore, KY Einstein, ME Cheng, HW Rubin, DA AF Hester, P. Y. Enneking, S. A. Jefferson-Moore, K. Y. Einstein, M. E. Cheng, H. W. Rubin, D. A. TI The effect of perches in cages during pullet rearing and egg laying on hen performance, foot health, and plumage SO POULTRY SCIENCE LA English DT Article DE perch; pullet; laying hen; White Leghorn; egg production ID DIFFERENT HOUSING SYSTEMS; CONVENTIONAL CAGES; FURNISHED CAGES; DOMESTIC-FOWL; BODY WEIGHT; BEHAVIOR; WELFARE; DESIGN; SELECTION; QUALITY AB Enrichment of pullet cages with perches has not been studied. Our objective was to determine if access to metal perches during all or part of the life cycle of caged White Leghorns affected egg traits, foot health, and feather condition. Treatment 1 represented control chickens that never had access to perches during their life cycle. Treatment 2 hens had perches only during the egg laying phase of the life cycle (17 to 71 wk of age), whereas treatment 3 chickens had perches during the pullet phase (0 to 16.9 wk of age). Treatment 4 chickens always had access to perches (0 to 71 wk of age). Comparisons between chickens that always had perches with controls that never had perches showed similar performance relative to egg production, cracked eggs, egg weight, shell weight, % shell, and shell thickness. More dirty eggs occurred in laying cages with perches. Feed usage increased resulting in poorer feed efficiency in hens with perch exposure during the pullet phase with no effect during egg laying. Perches did not affect hyperkeratosis of toes and feet. The back claw at 71 wk of age broke less if hens had prior experience with perches during the pullet phase. In contrast, during egg laying, the back claw at 71 wk of age broke more due to the presence of perches in laying cages. Perches in laying cages resulted in shorter trimmed claws and improved back feather scores, but caused poorer breast and tail feather scores. In conclusion, enriching conventional cages with perches during the entire life cycle resulted in similar hen performance compared with controls. Fewer broken back claws but poorer feed efficiency occurred because of prior experience with perches as pullets. Perch presence during egg laying improved back feather scores with more trimmed nails but caused more dirty eggs, broken back claws, and poorer breast and tail feather scores. Although perches allow chickens to express their natural perching instinct, it was not without causing welfare problems. C1 [Hester, P. Y.; Enneking, S. A.; Einstein, M. E.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Jefferson-Moore, K. Y.] North Carolina Agr & Tech State Univ, Greensboro, NC 27411 USA. [Cheng, H. W.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Rubin, D. A.] Illinois State Univ, Normal, IL 61790 USA. RP Hester, PY (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. EM phester@purdue.edu FU Agriculture and Food Research Initiative competitive grant from the USDA National Institute of Food and Agriculture [2011-67021-30114] FX This project was supported by Agriculture and Food Research Initiative competitive grant no. 2011-67021-30114 from the USDA National Institute of Food and Agriculture. We thank F. A. Haan and B. D. Little (Purdue University, West Lafayette, IN) for the management and care of the birds. Perches were donated by T. L. Pollard of Big Dutchman (Holland, MI), and the hatchlings were provided by Hy-Line Hatchery (Warren, IN). NR 33 TC 16 Z9 16 U1 5 U2 52 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 310 EP 320 DI 10.3382/ps.2012-02744 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900004 PM 23300294 ER PT J AU Kuttappan, VA Huff, GR Huff, WE Hargis, BM Apple, JK Coon, C Owens, CM AF Kuttappan, V. A. Huff, G. R. Huff, W. E. Hargis, B. M. Apple, J. K. Coon, C. Owens, C. M. TI Comparison of hematologic and serologic profiles of broiler birds with normal and severe degrees of white striping in breast fillets SO POULTRY SCIENCE LA English DT Article DE white striping; hematologic; serologic; liver; abdominal fat pad ID SKELETAL-MUSCLE; ADIPOSE-TISSUE; FAT DEPOSITION; FIBRO/ADIPOGENIC PROGENITORS; ABDOMINAL FAT; MEAT QUALITY; DIETARY-FAT; BODY-WEIGHT; TURKEY; CHICKENS AB White striping is the white striation occasionally observed parallel to the direction of muscle fibers in broiler breast fillets and thighs at the processing plant. Broiler breast fillets can be categorized as normal (NORM), moderate (MOD), or severe (SEV) based on the degree of white striping. Histologically, SEV fillets are characterized by the highest degree of degeneration of muscle fibers along with fibrosis and lipidosis when compared with NORM. The present study was undertaken to compare the hematologic and serologic profiles of broilers with NORM and SEV degrees of white striping to get more information on the systemic changes associated with the condition. Day-old male broiler chicks of a commercial strain were grown on the same diet in 6 replicate pens (n = 32 birds/pen). Blood samples (5 mL) were collected from the wing vein of each bird on the day before processing for analyzing hematologic and serologic profiles. At 63 d, the birds were weighed and processed in a commercial inline processing system. Weight of the butterfly fillets, liver, and abdominal fat pad were recorded. Left-side fillets were scored to obtain the degree of white striping for each bird. Representative samples for NORM (n = 24) and SEV (n = 17) categories were selected to compare the hematologic and serologic profiles. The SEV birds had greater (P < 0.05) live, fillet, and liver weights, as well as fillet yield, compared with the NORM birds, but the abdominal fat yield was less (P < 0.05) in SEV birds. The NORM and SEV birds did not show any differences in various hematological parameters, including the differential leukocyte count. Conversely, SEV birds had elevated (P < 0.05) serum levels of creatine kinase, alanine transaminase, aspartate aminotransferase, and lactate dehydrogenase. These results suggest that there is no systemic infectious or inflammatory condition associated with a SEV degree of white striping. The elevated serum enzyme levels confirm the muscle damage associated with the degenerative myopathy in SEV birds. C1 [Kuttappan, V. A.; Hargis, B. M.; Coon, C.; Owens, C. M.] Univ Arkansas, Dept Poultry Sci, Div Agr, Fayetteville, AR 72701 USA. [Huff, G. R.; Huff, W. E.] USDA, ARS, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. [Apple, J. K.] Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR 72701 USA. RP Owens, CM (reprint author), Univ Arkansas, Dept Poultry Sci, Div Agr, Fayetteville, AR 72701 USA. EM cmowens@uark.edu NR 41 TC 11 Z9 11 U1 4 U2 33 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 339 EP 345 DI 10.3382/ps.2012-02647 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900008 PM 23300298 ER PT J AU Gast, RK Guraya, R Jones, DR Anderson, KE AF Gast, Richard K. Guraya, Rupa Jones, Deana R. Anderson, Kenneth E. TI Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in conventional or enriched cages SO POULTRY SCIENCE LA English DT Article DE Salmonella Enteritidis; chicken; internal organ; conventional cage; enriched cage ID ENTERICA SEROTYPE ENTERITIDIS; DIFFERENT HOUSING SYSTEMS; RISK-FACTORS; PHAGE TYPE-4; UNITED-STATES; HORIZONTAL TRANSMISSION; REPRODUCTIVE-TRACT; LAYER FLOCKS; EGGS LAID; CONTAMINATION AB More human illnesses caused by Salmonella enterica subspecies enterica serovar Enteritidis throughout the world have been linked to the consumption of contaminated eggs than to any other food vehicle. Deposition of this pathogen in the edible contents of eggs occurs when systemic infections of laying hens involve colonization of reproductive organs. In recent years, the consequences of different housing systems for laying flocks have become the focus of international attention from both animal welfare and public health perspectives. Nevertheless, many questions remain unresolved regarding the food safety implications of various laying hen production systems. The present study assessed the effects of 2 different housing types (conventional cages and colony cages enriched with perching, nesting, and scratching areas) on the invasion of internal organs by Salmonella Enteritidis in experimentally infected laying hens. In 2 trials, groups of laying hens housed in each cage system were orally inoculated with doses of 1.0 x 10(7) cfu of Salmonella Enteritidis. At 5 to 6 d postinoculation, hens were euthanized and samples of internal organs were removed for bacteriologic culturing. For both trials combined, Salmonella Enteritidis was recovered from 95.3% of cecal samples, with no significant differences observed between housing systems. However, Salmonella Enteritidis was detected at significantly (P < 0.05) higher frequencies from hens in conventional cages than from hens in enriched cages for samples of livers (96.9 vs. 75.0%), spleens (93.8 vs. 53.1%), ovaries (25.0 vs. 10.4%), and oviducts (19.8 vs. 2.1%). These results demonstrate that differences in housing systems for egg-laying flocks can affect the susceptibility of hens to colonization of internal organs by Salmonella Enteritidis. C1 [Gast, Richard K.; Guraya, Rupa; Jones, Deana R.] USDA, ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Anderson, Kenneth E.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC 27695 USA. RP Gast, RK (reprint author), USDA, ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. EM Richard.Gast@ars.usda.gov NR 55 TC 8 Z9 8 U1 1 U2 22 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 468 EP 473 DI 10.3382/ps.2012-02811 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900025 PM 23300315 ER PT J AU Venkitanarayanan, K Kollanoor-Johny, A Darre, MJ Donoghue, AM Donoghue, DJ AF Venkitanarayanan, K. Kollanoor-Johny, A. Darre, M. J. Donoghue, A. M. Donoghue, D. J. TI Use of plant-derived antimicrobials for improving the safety of poultry products SO POULTRY SCIENCE LA English DT Article DE Salmonella Enteritidis; Campylobacter jejuni; chicken; cecum; plant-derived antimicrobial ID SALMONELLA-ENTERITIDIS COLONIZATION; CAMPYLOBACTER-JEJUNI COLONIZATION; CHAIN FATTY-ACIDS; MUCOSAL COMPETITIVE-EXCLUSION; ENTERICA SEROTYPE ENTERITIDIS; SYNTHETIC FOOD-ADDITIVES; CHICKEN CECAL CONTENTS; TEA TREE OIL; BROILER-CHICKENS; UNITED-STATES AB Salmonella Enteritidis and Campylobacter jejuni are the 2 major foodborne pathogens transmitted through poultry products. Chickens are the reservoir hosts of these pathogens, with their intestinal colonization being the most significant factor causing contamination of meat and eggs. Effective preslaughter strategies for reducing the colonization of birds with these pathogens are critical to improve the microbiological safety of poultry products. An antimicrobial treatment that can be applied through feed represents the most on farms. Additionally, a natural and safe antimicrobial will be better accepted by producers without concerns for toxicity. This symposium talk discussed the potential use of plant-derived, GRAS (generally recognized as safe)-status molecules, caprylic acid, trans-cinnamaldehyde, eugenol, carvacrol, and thymol as feed supplements for reducing cecal populations of Salmonella Enteritidis and C. jejuni in chickens. Additionally, the effect of plant molecules on Salmonella virulence genes critical for cecal colonization in chickens was also discussed. C1 [Venkitanarayanan, K.; Kollanoor-Johny, A.; Darre, M. J.] Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA. [Donoghue, A. M.] USDA, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. [Donoghue, D. J.] Univ Arkansas, Ctr Excellence Poultry Sci, Fayetteville, AR 72701 USA. RP Venkitanarayanan, K (reprint author), Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA. EM kumar.venkitanarayanan@uconn.edu RI Kollanoor Johny, Anup/J-3771-2015 NR 104 TC 11 Z9 11 U1 1 U2 36 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 493 EP 501 DI 10.3382/ps.2012-02764 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900029 PM 23300319 ER PT J AU Seal, BS AF Seal, Bruce S. TI Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium SO POULTRY SCIENCE LA English DT Article DE enzybiotic; antibiotic alternative; bacterial virus; food safety; animal/human health ID STAPHYLOCOCCUS-AUREUS; BROILER-CHICKENS; DRUG DISCOVERY; RESISTANCE; EVOLUTION; POULTRY; ANTIMICROBIALS; LYSOSTAPHIN; EXPRESSION; ENDOLYSINS AB There has been a resurgent interest in the use of bacteriophages or their gene products to control bacterial pathogens as alternatives to currently used antibiotics. Clostridium perfringens is a gram-positive, spore-forming anaerobic bacterium that plays a significant role in human foodborne disease as well as non-foodborne human, animal, and avian diseases. Countries that have complied with the ban on antimicrobial growth promoters in feeds have reported increased incidences of C. perfringens-associated diseases in poultry. To address these issues, new antimicrobial agents, putative lysins encoded by the genomes of bacteriophages, are being identified in our laboratory. Poultry intestinal material, soil, sewage, and poultry processing drainage water were screened for virulent bacteriophages that could lyse C. perfringens and produce clear plaques in spot assays. Bacteriophages were isolated that had long noncontractile tails, members of the family Siphoviridae, and with short noncontractile tails, members of the family Podoviridae. Several bacteriophage genes were identified that encoded N-acetylmuramoyl-L-alanine amidases, lysozyme-endopeptidases, and a zinc carboxypeptidase domain that has not been previously reported in viral genomes. Putative phage lysin genes (ply) were cloned and expressed in Escherichia coli. The recombinant lysins were amidases capable of lysing both parental phage host strains of C. perfringens as well as other strains of the bacterium in spot and turbidity reduction assays, but did not lyse any clostridia beyond the species. Consequently, bacteriophage gene products could eventually be used to target bacterial pathogens, such as C. perfringens via a species-specific strategy, to control animal and human diseases without having deleterious effects on beneficial probiotic bacteria. C1 ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. RP Seal, BS (reprint author), ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM bruce.seal@ars.usda.gov FU USDA Agricultural Research Service (ARS, Current Research Information System) [6612-32000-060]; Russian Federation State Research Center for Applied Microbiology and Biotechnology; US Department of State via the International Science and Technology Center (ISTC) [3108]; National Institutes of Health (NIH) Grant from the IDeA Network of Biomedical Research Excellence Program of the National Center for Research Resources [P20 RR-016464]; NIH grant [1S10 RR023587-01A2]; US Department of State via the ARS, USDA Office of International Research Programs (OIRP) FX Appreciation is extended to all authors on collaborative research cited in this manuscript. Support for the research was provided by the USDA Agricultural Research Service (ARS, Current Research Information System project #6612-32000-060), the Russian Federation State Research Center for Applied Microbiology and Biotechnology, and the US Department of State via the International Science and Technology Center (ISTC grant #3108) via the ARS, USDA Office of International Research Programs (OIRP) with the administrative support of Melanie Peterson (ARS, OIRP) and Patrick Russo (ISTC). The Nevada Proteomics Center operates under the auspices of National Institutes of Health (NIH) Grant #P20 RR-016464 from the IDeA Network of Biomedical Research Excellence Program of the National Center for Research Resources with instrumentation acquired through NIH grant #1S10 RR023587-01A2 awarded to the University of Nevada, Reno. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the ARS, USDA. NR 52 TC 10 Z9 10 U1 1 U2 40 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2013 VL 92 IS 2 BP 526 EP 533 DI 10.3382/ps.2012-02708 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149JB UT WOS:000319313900031 PM 23300321 ER PT J AU Traesel, CK Silva, MSE Spilki, FR Weiblen, R Flores, EF AF Traesel, Carolina Kist Sa e Silva, Mariana Spilki, Fernando Rosado Weiblen, Rudi Flores, Eduardo Furtado TI Nucleotide sequencing and phylogenetic analysis of the 3 ' region of glycoprotein C gene of South American bovine herpesviruses 1 and 5 SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE BoHV-1; BoHV-5; gC; Transmembrane helix; Cytoplasmic tail ID VIRAL DIARRHEA VIRUS; CYTOPLASMIC DOMAIN; NEUROLOGICAL DISEASE; TOPOLOGY PREDICTION; LATENT INFECTION; IBR VIRUS; TYPE-1; DIFFERENTIATION; PROTEIN; CALVES AB We herein describe a molecular analysis based on the 3' region of the glycoprotein C gene of 45 bovine herpesvirus (BoHV) isolates from Brazil (n = 41), Uruguay (n = 2) and Argentina (n = 2). Nucleotide (nt) sequencing and alignment of 333 nt revealed levels of similarity ranging from 99.1% to 100% among BoHV-1 sequences (n = 12); 96.2-100% among BoHV-5 sequences (n = 32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The isolate SV 453/93 (BoHV-1 associated with genital disease) could not be included within BoHV-1 subtypes since it presented a markedly distinct nt and amino acid (aa) deduced sequences. A transmembrane domain of 24 aa and the putative cytoplasmic tail of 8 aa were identified and mapped. These results indicate that this genome region represents a suitable target for phylogenetic subgrouping of BoHV-1 and BoHV-5 isolates and, perhaps, for understanding evolutionary relationships. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Traesel, Carolina Kist; Weiblen, Rudi; Flores, Eduardo Furtado] Univ Fed Santa Maria, Dept Med Vet Prevent, Setor Virol, BR-97105900 Santa Maria, RS, Brazil. [Sa e Silva, Mariana] ARS, Dept Agr, USDA, Southeast Poultry Res Lab, Athens, GA USA. [Spilki, Fernando Rosado] Univ Feevale, Mol Microbiol Lab, Novo Hamburgo, RS, Brazil. RP Flores, EF (reprint author), Univ Fed Santa Maria, Dept Med Vet Prevent, Setor Virol, Av Roraima 1000,Predio 20,Sala 4200, BR-97105900 Santa Maria, RS, Brazil. EM eduardofurtadoflores@gmail.com RI Weiblen, Rudi/H-7003-2014; Spilki, Fernando/E-3736-2010; flores, eduardo/H-5125-2016 OI Weiblen, Rudi/0000-0002-1737-9817; Spilki, Fernando/0000-0001-5804-7045; FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX This study was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). E.F. Flores, R. Weiblen and F.R. Spilki are CNPq research fellows. We are grateful to Dr. Marcelo de Lima for assisting with hydropathicity analysis. NR 41 TC 4 Z9 4 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD FEB PY 2013 VL 94 IS 1 BP 178 EP 185 DI 10.1016/j.rvsc.2012.07.032 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 145PB UT WOS:000319028000027 PM 22929314 ER PT J AU Burns, ES Toth, SF Haight, RG AF Burns, Eileen S. Toth, Sandor F. Haight, Robert G. TI A modeling framework for life history-based conservation planning SO BIOLOGICAL CONSERVATION LA English DT Article DE Reserve design; Life history-based protection; Spatial optimization; 0-1 Programming ID RESERVE SITE SELECTION; HABITAT SUITABILITY; BIODIVERSITY CONSERVATION; LANDSCAPE CONNECTIVITY; FOREST MANAGEMENT; OPTIMIZATION; CHALLENGES; SYSTEM; TOOLS; BATS AB Reserve site selection models can be enhanced by including habitat conditions that populations need for food, shelter, and reproduction. We present a new population protection function that determines whether minimum areas of land with desired habitat features are present within the desired spatial conditions in the protected sites. Embedding the protection function as a constraint in reserve site selection models provides a way to select sets of sites that satisfy these habitat requirements. We illustrate the mechanics and the flexibility of the protection function by embedding it in two linear-integer programming models for reserve site selection and applying the models to a case study of Myotis bat conservation on Lopez Island, United States. The models capture high-resolution, species-specific habitat requirements that are critical for Myotis persistence. The models help quantify the increasing marginal costs of protecting Myotis habitat and show that optimal site selection strategies are sensitive to the relative importance of habitat requirements. (c) 2012 Elsevier Ltd. All rights reserved. C1 [Burns, Eileen S.; Toth, Sandor F.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Haight, Robert G.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Toth, SF (reprint author), Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM toths@uw.edu; rhaight@fs.fed.us FU U.S. Forest Service, Northern Research Station FX We thank J. Aukema, J. Marzluff, C. Montgomery, S. Snyder, and the referees for constructive comments on the manuscript. We thank the U.S. Forest Service, Northern Research Station for providing financial support for this study. We thank the members and friends of the Quantitative Ecology & Resource Management program at the University of Washington for their support, and many members of ICES for their feedback and encouragement. NR 56 TC 3 Z9 3 U1 0 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 J9 BIOL CONSERV JI Biol. Conserv. PD FEB PY 2013 VL 158 BP 14 EP 25 DI 10.1016/j.biocon.2012.08.009 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 133HA UT WOS:000318128300002 ER PT J AU Longcore, T Rich, C Mineau, P MacDonald, B Bert, DG Sullivan, LM Mutrie, E Gauthreaux, SA Avery, ML Crawford, RL Manville, AM Travis, ER Drake, D AF Longcore, Travis Rich, Catherine Mineau, Pierre MacDonald, Beau Bert, Daniel G. Sullivan, Lauren M. Mutrie, Erin Gauthreaux, Sidney A., Jr. Avery, Michael L. Crawford, Robert L. Manville, Albert M., II Travis, Emilie R. Drake, David TI Avian mortality at communication towers in the United States and Canada: which species, how many, and where? SO BIOLOGICAL CONSERVATION LA English DT Article DE Communication towers; Mortality; Night lighting; Neotropical migrants; Collisions; Impact assessment ID POPULATION DECLINES; NOCTURNAL MIGRANTS; TELEVISION TOWER; TV TOWER; BIRDS; MIGRATION; COLLISIONS; ILLINOIS; LIGHTS; POWER AB Birds migrating to and from breeding grounds in the United States and Canada are killed by the millions in collisions with lighted towers and their guy wires. Avian mortality at towers is highly variable across species, and the importance to each population depends on its size and trajectory. Building on our previous estimate of avian mortality at communication towers, we calculated mortality by species and by regions. To do this, we constructed a database of mortality by species at towers from available records and calculated the mean proportion of each species killed at towers within aggregated Bird Conservation Regions. These proportions were combined with mortality estimates that we previously calculated for those regions. We then compared our estimated bird mortality rates to the estimated populations of these species in the United States and Canada. Neotropical migrants suffer the greatest mortality; 97.4% of birds killed are passerines, mostly warblers (Parulidae, 58.4%), vireos (Vireonidae, 13.4%), thrushes (Turdidae, 7.7%), and sparrows (Emberizidae, 5.8%). Thirteen birds of conservation concern in the United States or Canada suffer annual mortality of 1-9% of their estimated total population. Of these, estimated annual mortality is >2% for Yellow Rail (Coturnicops noveboracensis), Swainson's Warbler (Limnothlypis swainsonii), Pied-billed Grebe (Podilymbus podiceps), Bay-breasted Warbler (Setophaga castanea), Golden-winged Warbler (Vermivora chrysoptera), Worm-eating Warbler (Helmitheros vermivorum), Prairie Warbler (Setophaga discolor), and Ovenbird (Seiurus aurocapilla). Avian mortality from anthropogenic sources is almost always reported in the aggregate ("number of birds killed"), which cannot detect the species-level effects necessary to make conservation assessments. Our approach to per species estimates could be undertaken for other sources of chronic anthropogenic mortality. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Longcore, Travis; Rich, Catherine; MacDonald, Beau] Urban Wildlands Grp, Los Angeles, CA 90024 USA. [Longcore, Travis] Univ So Calif, Spatial Sci Inst, Los Angeles, CA 90089 USA. [Mineau, Pierre; Mutrie, Erin] Environm Canada, Natl Wildlife Res Ctr, Sci & Technol Branch, Ottawa, ON, Canada. [Bert, Daniel G.] Carleton Univ, Geomat & Landscape Ecol Res Lab, Ottawa, ON K1S 5B6, Canada. [Sullivan, Lauren M.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Gauthreaux, Sidney A., Jr.] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA. [Avery, Michael L.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL 32641 USA. [Manville, Albert M., II] US Fish & Wildlife Serv, Div Migratory Bird Management, Arlington, VA 22203 USA. [Travis, Emilie R.; Drake, David] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Longcore, T (reprint author), Urban Wildlands Grp, POB 24020, Los Angeles, CA 90024 USA. EM longcore@urbanwildlands.org; crich@urbanwildlands.org; Pierre.Mineau@ec.gc.ca; beaumacdonald@sbcglobal.net; dan.bert@glel.carleton.ca; laurenmsullivan@gmail.com; er339081@dal.ca; gocajuns@bellsouth.net; Michael.LAvery@aphis.usda.gov; rlcrawfd@rose.net; Albert_Manville@fws.gov; uvem05@gmail.com; ddrake2@wisc.edu RI Longcore, Travis/A-4978-2008 OI Longcore, Travis/0000-0002-1039-2613 FU Environment Canada; American Bird Conservancy; Defenders of Wildlife FX The authors acknowledge the outstanding contribution of Herbert L. Stoddard and Tall Timbers Research Station in executing the long-term study of avian mortality at the WCTV tower, for which we had access to the data. Environment Canada, American Bird Conservancy, and Defenders of Wildlife provided financial support for the early stages of this research. The authors thank Gerald Winegrad, Caroline Kennedy, Joelle Gehring, and Eugene A. Young for productive discussions about this research and access to unpublished reports. NR 140 TC 35 Z9 38 U1 8 U2 78 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 J9 BIOL CONSERV JI Biol. Conserv. PD FEB PY 2013 VL 158 BP 410 EP 419 DI 10.1016/j.biocon.2012.09.019 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 133HA UT WOS:000318128300045 ER PT J AU Kaczmarek, DJ Coyle, DR Coleman, MD AF Kaczmarek, Donald J. Coyle, David R. Coleman, Mark D. TI Survival and growth of a range of Populus clones in central South Carolina USA through age ten: Do early assessments reflect longer-term survival and growth trends? SO BIOMASS & BIOENERGY LA English DT Article DE Short-rotation woody crops; Bioenergy feedstocks; Populus deltoides; Hybrid poplar; Productivity; Clonal selection ID HYBRID POPLAR CLONES; ROTATION WOODY CROPS; UNITED-STATES; PRODUCTION PHYSIOLOGY; BIOMASS PRODUCTION; DELTOIDES HYBRIDS; ENERGY CROPS; PRODUCTIVITY; PLANTATIONS; TRICHOCARPA AB Increased emphasis is being placed on developing bioenergy production capacity in the United States and Populus species or hybrids have been identified as strong candidates for the southeastern United States. Thirty-one Populus clones were planted in South Carolina and survival and growth assessments were completed after the 1st, 2nd, 3rd, and 10th growing seasons. Survival and growth differed significantly among clones. Age-to-age correlations for survival and growth traits were made and results suggest that for most clones, age 10 survival was strongly correlated with first-year survival. A small group of clones exhibited delayed mortality indicating poor longer-term adaptability. For growth, clonal selections based on age 3 volumes could be made and rank changes involve moderate to poorly performing clones. Clones with the greatest age 3 volumes, 184-411, WV416, and 52-225, ranked 1st, 2nd, and 3rd for age 10 volume. These clones represent both T x D hybrids and pure Populus deltoides clones suggesting growth is a function of specific clonal characteristics rather than overall species or hybrid traits. The growth obtained under these test conditions is generally less than the maximum growth rates of the same clones obtained in various tests. Potential reasons for this variation are discussed. To develop Populus as a commercially viable bioenergy crop for the southeastern United States, concurrent research to identify site requirements, adaptability, and appropriate management regimes is needed. Further breeding and clonal selection will be critically important, but it is unlikely that this alone will be sufficient to assure high productivity. Published by Elsevier Ltd. C1 [Kaczmarek, Donald J.] US Forest Serv, USDA, USFS SRWC, Aiken, SC 29803 USA. [Coyle, David R.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Coleman, Mark D.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. RP Kaczmarek, DJ (reprint author), US Forest Serv, USDA, USFS SRWC, Savannah River Res Campus,241 Gateway Dr, Aiken, SC 29803 USA. EM djkaczmarek@fs.fed.us; dcoyle@warnell.uga.edu; mcoleman@uidaho.edu RI Coleman, Mark/A-6741-2013 FU Savannah River Ecology Laboratory (UGA); Department of Energy-Savannah River Operations Office through the U.S. Forest Service Savannah River; Department of Energy-Savannah River Operations Office through Forest Service Southern Research Station under Interagency Agreement [DE-IA09-00SR22188]; Department of Energy Oak Ridge National Laboratory Interagency Agreement [00-IA-11330135-221]; Southern Research Station Research Work Unit 4153; Timber Company, Weyerhaeuser, Champion International, and Union Camp FX We thank the many technicians who have assisted with this project including Tucker Slack, Justin Cumbee, and Laura Krysinsky (USDA Forest Service Savannah River). Special thanks to the Savannah River Ecology Laboratory (UGA) for project support and use of equipment and facilities. Funding was provided by the Department of Energy-Savannah River Operations Office through the U.S. Forest Service Savannah River and the Forest Service Southern Research Station under Interagency Agreement DE-IA09-00SR22188; Department of Energy Oak Ridge National Laboratory Interagency Agreement 00-IA-11330135-221; Southern Research Station Research Work Unit 4153 and The Timber Company, Weyerhaeuser, Champion International, and Union Camp. We would also like to thank Tom Blush and Jeff Wright for their helpful reviews of an earlier draft of this manuscript. Three additional anonymous reviewers and the editor provided thoughtful suggestions and this paper has been strengthened by their contributions. NR 54 TC 9 Z9 9 U1 1 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD FEB PY 2013 VL 49 BP 260 EP 272 DI 10.1016/j.biombioe.2012.12.005 PG 13 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 130CA UT WOS:000317889100028 ER PT J AU Horie, T Haight, RG Homans, FR Venette, RC AF Horie, Tetsuya Haight, Robert G. Homans, Frances R. Venette, Robert C. TI Optimal strategies for the surveillance and control of forest pathogens: A case study with oak wilt SO ECOLOGICAL ECONOMICS LA English DT Article DE Forest pathogens; Surveillance; Optimal management; Uncertainty; Ceratocystis fagacearum ID INVASIVE SPECIES MANAGEMENT; DISEASE; MODEL; ECONOMICS; IMPACTS AB Cost-effective strategies are needed to find and remove diseased trees in forests damaged by pathogens. We develop a model of cost-minimizing surveillance and control of forest pathogens across multiple sites where there is uncertainty about the extent of the infestation in each site and when the goal is to minimize the expected number of new infections. We allow for a heterogeneous landscape, where grid cells may be differentiated by the number of trees, the expected number of infected trees, rates of infection growth, and costs of surveillance and control. In our application to oak wilt in Anoka County, Minnesota, USA, we develop a cost curve associated with saving healthy trees from infection. Assuming an annual infection growth rate of 8%, a $1 million budget would save an expected 185 trees from infection for an average of $5400 per tree. We investigate how more precise prior estimates of disease and reduced detection sensitivity affect model performance. We evaluate rules of thumb, finding that prioritizing sites with high proportions of infected trees is best. Our model provides practical guidance about the spatial allocation of surveillance and control resources for well-studied forest pathogens when only modest information about their geographic distribution is available. (C) 2012 Elsevier B.V. All rights reserved. C1 [Horie, Tetsuya] Sophia Univ, Grad Sch Global Environm Studies, Chiyoda Ku, Tokyo 1028554, Japan. [Haight, Robert G.; Venette, Robert C.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Homans, Frances R.] Univ Minnesota, Dept Appl Econ, St Paul, MN 55108 USA. RP Homans, FR (reprint author), Univ Minnesota, Dept Appl Econ, 1994 Buford Ave,217G Ruttan Hall, St Paul, MN 55108 USA. EM fhomans@umn.edu FU U.S. Forest Service; Northern Research Station; Minnesota Agricultural Experiment Station; Economic Research Service's (USDA) PREISM economics of invasive species management program [58-7000-6-0081] FX This article was partially produced under a co-operative agreement (number 58-7000-6-0081) with the Economic Research Service's (USDA) PREISM economics of invasive species management program. This research has also been supported by the U.S. Forest Service, Northern Research Station and the Minnesota Agricultural Experiment Station. The authors thank Susan Burks of the Minnesota Department of Natural Resources for providing information on the distribution of oak wilt, D. Bengston, K. Kovacs, and S. Snyder for valuable pre-submission reviews, and three anonymous referees for valuable comments. NR 30 TC 9 Z9 9 U1 5 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 J9 ECOL ECON JI Ecol. Econ. PD FEB PY 2013 VL 86 BP 78 EP 85 DI 10.1016/j.ecolecon.2012.09.017 PG 8 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA 128XR UT WOS:000317803500009 ER PT J AU Lockhart, BR AF Lockhart, Brian Roy TI Site Index Determination Techniques for Southern Bottomland Hardwoods SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE species trials; soil-site index equations; soil series; expert systems; Baker/Broadfoot system ID SOIL; HEIGHT; CURVES; GROWTH; FOREST AB Site index is a species-specific indirect measure of forest productivity expressed as the average height of dominant and codominant trees in a stand at a specified base age. It is widely used by forest managers to make informed decisions regarding forest management practices. Unfortunately, forest managers have difficulty in determining site index for southern US bottomland hardwood stands because of a lack of available information for many tree species, outdated information for several techniques, and a lack of knowledge in the application of other techniques for specific site conditions. Techniques to determine site index in bottomland hardwood stands include species trials, site index curves, soil-site equations, soil series, and expert systems. Each technique is reviewed here, including recent advances if available, advantages, disadvantages, and application to bottomland hardwood stands. C1 US Forest Serv, USDA, Southern Res Stn, Stoneville, MS USA. RP Lockhart, BR (reprint author), US Forest Serv, USDA, Southern Res Stn, Stoneville, MS USA. EM blockhart@fs.fed.us NR 62 TC 0 Z9 0 U1 2 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD FEB PY 2013 VL 37 IS 1 BP 5 EP 12 DI 10.5849/sjaf.09-027 PG 8 WC Forestry SC Forestry GA 130YZ UT WOS:000317958000002 ER PT J AU Secoges, JM Aust, WM Seiler, JR Dolloff, CA Lakel, WA AF Secoges, Joseph M. Aust, Wallace M. Seiler, John R. Dolloff, C. Andrew Lakel, William A. TI Streamside Management Zones Affect Movement of Silvicultural Nitrogen and Phosphorus Fertilizers to Piedmont Streams SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE riparian forests; fertilization; forest operations; best management practices; water quality ID ANION-EXCHANGE MEMBRANES; WATER-QUALITY; FOREST FERTILIZATION; RIPARIAN FOREST; UNITED-STATES; COASTAL-PLAIN; SEDIMENT; BUFFER; VEGETATION; POLLUTION AB Forestry best management practices (BMP) recommendations for streamside management zones (SMZs) are based on limited data regarding SMZ width, partial harvests, and nutrient movements after forest fertilization. Agricultural fertilization is commonly linked to increased stream nutrients. However, less is known about effectiveness of SMZ options for controlling nutrient movements after silvicultural fertilization. Diammonium phosphate and urea were applied to 12 subwatersheds in 3-year-old loblolly pine (Pinus taeda L.) plantations in the Virginia Piedmont. Three replicates of four SMZ treatments were superimposed on 12 subwatersheds in a previous SMZ harvest sediment study (7.6-m SMZ, 15.2-m SMZ thin, 15.2-m SMZ, and 30.5-m SMZ). Surface, near-surface, subsurface, and stream water samples were collected monthly for 1 year and analyzed for nitrate (NO3-), ammonium (NH4+), and orthophosphate (ortho-P). Transected measurements from streamside to fertilized plantations allowed interpretations of spatial nutrient measurements across SMZs. When compared with wider SMZs, 7.6-m SMZs had 3-10X surface water NO3-, 3-6X near-surface water NO3-, and 1-2X more stream water NO3-. No significant differences were detected for NH4+ for any SMZ treatment. The 15.2-m SMZ thin had small but significant increases (2-8X) in surface runoff for ortho-P relative to other SMZ treatments, perhaps because of increased surface water movement along thinning corridors. Across all SMZ treatments, comparisons of stream edges with fertilized stands indicated NO3- reductions of 33-98%, NH4+ reductions of 68-97%, and ortho-P reductions of 70-98%. A 39% rainfall deficit during the study influenced results, but conventional SMZs >= 15.2 m protected streams from fertilization nutrient increases. C1 [Secoges, Joseph M.] Ellett Valley Forest Management LLC, Blacksburg, VA USA. [Aust, Wallace M.; Seiler, John R.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Dolloff, C. Andrew] US Forest Serv, USDA, Washington, DC USA. RP Secoges, JM (reprint author), Ellett Valley Forest Management LLC, Blacksburg, VA USA. EM waust@vt.edu FU National Council for Air and Stream Improvement (NCASI), Inc.; USDA Forest Service; MeadWestvaco Corp.; Virginia Tech. FX Joseph M. Secoges, Ellett Valley Forest Management, LLC. Wallace M. Aust (waust@vt.edu), Virginia Tech, Department of Forest Resources and Environmental Conservation, Blacksburg, VA. John R. Seiler, Virginia Tech, Department of Forest Resources and Environmental Conservation, Blacksburg, VA. C. Andrew Dolloff, USDA Forest Service. William A. Lakel, Virginia Department of Forestry. This research received financial and logistical support from the National Council for Air and Stream Improvement (NCASI), Inc., USDA Forest Service, MeadWestvaco Corp., and Virginia Tech. NR 65 TC 3 Z9 3 U1 7 U2 30 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD FEB PY 2013 VL 37 IS 1 BP 26 EP 35 DI 10.5849/sjaf.11-032 PG 10 WC Forestry SC Forestry GA 130YZ UT WOS:000317958000005 ER PT J AU Witt, EL Barton, CD Stringer, JW Bowker, DW Kolka, RK AF Witt, Emma L. Barton, Christopher D. Stringer, Jeff W. Bowker, Daniel W. Kolka, Randall K. TI Evaluating Best Management Practices for Ephemeral Stream Protection following Forest Harvest in the Cumberland Plateau SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE best management practices; streamside management zones; stream crossings; ephemeral streams ID HEADWATER STREAMS; WATER-QUALITY; SEDIMENT; CHANNELS; RUNOFF AB Most states in the United States have established streamside management zone (SMZ) recommendations for minimizing the impacts of forest operations on water quality and aquatic habitat. However, guidelines generally focus more on impacts to perennial streams than on ephemeral streams. An experiment was conducted to evaluate SMZ effectiveness on ephemeral streams in southeastern Kentucky. Ephemeral SMZ treatments included (1) harvest with no equipment limitation, no forest overstory retention, and use of unimproved stream crossings (noSMZ); (2) harvest with no equipment limitation, retention of channel bank trees, and use of improved stream crossings (SMZ1); (3) harvest with equipment restrictions within 7.6 m of the channel, retention of channel bank trees, and use of improved stream crossings (SMZ2); and (4) no harvest (control). Each treatment was replicated a minimum of three times (n of 3 to 6; 18 sites total) at the subwatershed level (0.75 to 8.92 ha). The improved stream crossing types studied included wooden portable skidder bridges, steel pipe/culverts, and PVC pipe bundles. Water samples were taken during storm flows and were analyzed for total suspended solids (TSS), turbidity, settleable solids, and sediment transport rate. Both the SMZ1 and SMZ2 treatments significantly reduced TSS and turbidity over the noSMZ treatment. Water in the SMZ1 treatment exhibited higher TSS and turbidity than the control, whereas the SMZ2 treatment was no different than the control for TSS but higher for turbidity. Use of any improved crossing in ephemeral streams significantly lowered TSS and turbidity compared to unimproved fords. Bridges yielded similar TSS and sediment transport levels as those exhibited by the control but turbidity was slightly higher. C1 [Witt, Emma L.; Stringer, Jeff W.] Univ Kentucky, Lexington, KY 40506 USA. [Barton, Christopher D.] Univ Kentucky, Dept Forestry, Lexington, KY 40546 USA. [Bowker, Daniel W.; Kolka, Randall K.] US Forest Serv, USDA, Washington, DC USA. RP Witt, EL (reprint author), Univ Kentucky, Lexington, KY 40506 USA. EM emma.witt@uky.edu; barton@uky.edu; stringer@uky.edu; dwbowk0@g.uky.edu; rkolka@fs.fed.us FU Kentucky Agricultural Experiment Station FX Emma L. Witt (emma.witt@uky.edu), University of Kentucky. Christopher D. Barton (barton@uky.edu), University of Kentucky, Department of Forestry, Lexington, KY. Jeff W. Stringer (stringer@uky.edu), University of Kentucky. Daniel W. Bowker (dwbowk0@g.uky.edu), USDA Forest Service. Randall K. Kolka (rkolka@fs.fed.us), USDA Forest Service. This work was supported in part by the Kentucky Agricultural Experiment Station. This paper is dedicated in memory of Will Marshall. NR 45 TC 3 Z9 3 U1 6 U2 20 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD FEB PY 2013 VL 37 IS 1 BP 36 EP 44 DI 10.5849/sjaf.11-041 PG 9 WC Forestry SC Forestry GA 130YZ UT WOS:000317958000006 ER PT J AU Burner, DM AF Burner, David M. TI Survival and Juvenile Growth of Loblolly and a Pitch x Loblolly Pine Hybrid in West-Central Arkansas SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE Pinus taeda; Pinus x rigitaeda AB Few studies have compared loblolly pine (Pinus taeda L.) and pitch (P. rigida Mill.) x loblolly pine hybrid (P. x rigitaeda Kartesz & Gandhi) in the west-central United States. The objective of this study was to compare planting survival and juvenile growth of loblolly pine and P. x rigitaeda on two soils near Booneville, Arkansas. Survival was associated with site and species, with most mortality occurring by 2 years postplanting. Loblolly pine had significantly greater survival (94%) than did P. x rigitaeda (82%). Loblolly pine also had greater height, dbh, basal area, and number of superior (top 25%) genotypes at 7 years postplanting for either dbh (>= 14.0 cm) or height (>= 6.6 m) than did P. x rigitaeda. Growth responses between species could become more evident as the stand matures, especially when influenced by ice-loading events. C1 USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR USA. RP Burner, DM (reprint author), USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR USA. EM David.Burner@ars.usda.gov NR 28 TC 1 Z9 1 U1 0 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD FEB PY 2013 VL 37 IS 1 BP 59 EP 62 DI 10.5849/sjaf.11-035 PG 4 WC Forestry SC Forestry GA 130YZ UT WOS:000317958000009 ER PT J AU Nsimba, RY Mullen, CA West, NM Boateng, AA AF Nsimba, Robert Y. Mullen, Charles A. West, Nathan M. Boateng, Akwasi A. TI Structure-Property Characteristics of Pyrolytic Lignins Derived from Fast Pyrolysis of a Lignin Rich Biomass Extract SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Etek Lignin; Fluidized Bed Fast Pyrolysis; Pyrolysis Oil Upgrading; Physicochemical Properties; Value-Added Chemicals ID WATER-INSOLUBLE FRACTION; BIO-OIL AB In this work, pyrolytic lignins isolated from the liquid product of the fast pyrolysis of Etek lignin were characterized using various analytical techniques. This is necessary to evaluate their potential usefulness as an aromatic chemical feedstock in a biomass pyrolysis scheme. Pyrolytic lignins were separated into various fractions by their solubility in water and dichloromethane. Results revealed that the thermal pyrolysis yielded lignin fractions of higher oxygen and lower carbon and hydrogen contents, leading to a decrease in their heating values. Pyrolytic lignin fractions were decarboxylated, had a higher concentration of aryl and alkyl hydroxyl moieties, and were lower molecular weight than the starting lignin. TGA analysis indicates that the pyrolytic lignins begin to thermally degrade at 120 degrees C. Overall the characterization data suggests that pyrolytic lignins obtained from the fast pyrolysis of Etek lignin are potentially useful feedstocks for sustainable production of renewable aromatic chemicals. C1 [Nsimba, Robert Y.; Mullen, Charles A.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [West, Nathan M.] Univ Sci, Dept Chem & Biochem, Philadelphia, PA 19104 USA. RP Boateng, AA (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov RI West, Nathan/C-5596-2014 OI West, Nathan/0000-0002-0657-3892 FU U.S. Department of Agriculture Specific Cooperative Agreement [58-1935-2-278] FX The authors are grateful to David B. Johnston, Guoping Bao, Heather Vanselous, Helen Ngo, Karen M. Wagner, and Victor T. Wyatt for their technical assistance and scientific cooperation. We thank the SEKAB E-Technology (Ornskoldsvik, Sweden) for the supply of Etek lignin. Author Nathan M. West would like to acknowledge funding support from the U.S. Department of Agriculture Specific Cooperative Agreement #58-1935-2-278. NR 24 TC 12 Z9 12 U1 1 U2 47 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD FEB PY 2013 VL 1 IS 2 BP 260 EP 267 DI 10.1021/sc300119s PG 8 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA 120CA UT WOS:000317145900009 ER PT J AU Nejat, N Vadamalai, G Davis, RE Harrison, NA Sijam, K Dickinson, M Abdullah, SNA Zhao, Y AF Nejat, Naghmeh Vadamalai, Ganesan Davis, Robert E. Harrison, Nigel A. Sijam, Kamaruzaman Dickinson, Matthew Abdullah, Siti Nor Akmar Zhao, Yan TI 'Candidatus Phytoplasma malaysianum', a novel taxon associated with virescence and phyllody of Madagascar periwinkle (Catharanthus roseus) SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID WITCHES-BROOM-DISEASE; SEQUENCE-VARIABLE MOSAICS; ASTER YELLOWS PHYTOPLASMA; 16S RIBOSOMAL-RNA; MOLECULAR CHARACTERIZATION; PHYLOGENETIC POSITIONS; ASH YELLOWS; GENES; CLASSIFICATION; GENOME AB This study addressed the taxonomic position and group classification of a phytoplasma responsible for virescence and phyllody symptoms in naturally diseased Madagascar periwinkle plants in western Malaysia. Unique regions in the 16S rRNA gene from the Malaysian periwinkle virescence (MaPV) phytoplasma distinguished the phytoplasma from all previously described 'Candidatus Phytoplasma' species. Pairwise sequence similarity scores, calculated through alignment of full-length 16S rRNA gene sequences, revealed that the MaPV phytoplasma 16S rRNA gene shared 96.5% or less sequence similarity with that of previously described 'Ca. Phytoplasma' species, justifying the recognition of the MaPV phytoplasma as a reference strain of a novel taxon, 'Candidatus Phytoplasma malaysianum'. The 16S rRNA gene F2nR2 fragment from the MaPV phytoplasma exhibited a distinct restriction fragment length polymorphism (RFLP) profile and the pattern similarity coefficient values were lower than 0.85 with representative phytoplasmas classified in any of the 31 previously delineated 16Sr groups; therefore, the MaPV phytoplasma was designated a member of a new 16Sr group, 16SrXXXII. Phytoplasmas affiliated with this novel taxon and the new group included diverse strains infecting periwinkle, coconut palm and oil palm in Malaysia. Three phytoplasmas were characterized as representatives of three distinct subgroups, 16SrXXXII-A, 16SrXXXII-B and 16SrXXXII-C, respectively. C1 [Nejat, Naghmeh; Vadamalai, Ganesan; Abdullah, Siti Nor Akmar] Univ Putra Malaysia, Inst Trop Agr, Fac Agr, Serdang 43400, Malaysia. [Vadamalai, Ganesan; Sijam, Kamaruzaman] Univ Putra Malaysia, Plant Protect Dept, Fac Agr, Serdang 43400, Malaysia. [Davis, Robert E.; Zhao, Yan] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Harrison, Nigel A.] Univ Florida, Ft Lauderdale Res & Educ Ctr, Dept Plant Pathol, Ft Lauderdale, FL 33314 USA. [Dickinson, Matthew] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England. [Abdullah, Siti Nor Akmar] Univ Putra Malaysia, Agr Technol Dept, Fac Agr, Serdang 43400, Malaysia. RP Zhao, Y (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov OI Dickinson, Matthew/0000-0002-7614-2609 NR 63 TC 20 Z9 21 U1 1 U2 11 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD FEB PY 2013 VL 63 BP 540 EP 548 DI 10.1099/ijs.0.041467-0 PN 2 PG 9 WC Microbiology SC Microbiology GA 120KY UT WOS:000317170400023 PM 22523165 ER PT J AU Davis, RE Zhao, Y Dally, EL Lee, IM Jomantiene, R Douglas, SM AF Davis, Robert E. Zhao, Yan Dally, Ellen L. Lee, Ing-Ming Jomantiene, Rasa Douglas, Sharon M. TI 'Candidatus Phytoplasma pruni', a novel taxon associated with X-disease of stone fruits, Prunus spp.: multilocus characterization based on 16S rRNA, secY, and ribosomal protein genes SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID MYCOPLASMALIKE ORGANISMS MLOS; SYMPTOM REMISSION; ASTER YELLOWS; PEACH TREES; CLASSIFICATION; IDENTIFICATION; BODIES; AMPLIFICATION; TETRACYCLINE; CICADELLIDAE AB X-disease is one of the most serious diseases known in peach (Prunus persica). Based on RFLP analysis of 16S rRNA gene sequences, peach X-disease phytoplasma strains from eastern and western United States and eastern Canada were classified in 16S rRNA gene RFLP group 16SrIII, subgroup A. Phylogenetic analyses of 16S rRNA gene sequences revealed that the X-disease phytoplasma strains formed a distinct subclade within the phytoplasma clade, supporting the hypothesis that they represented a lineage distinct from those of previously described 'Candidatus Phytoplasma' species. Nucleotide sequence alignments revealed that all studied X-disease phytoplasma strains shared less than 97.5% 16S rRNA gene sequence similarity with previously described 'Candidatus Phytoplasma' species. Based on unique properties of the DNA, we propose recognition of X-disease phytoplasma strain PX11CT1(R) as representative of a novel taxon, 'Candidatus Phytoplasma pruni'. Results from nucleotide and phylogenetic analyses of secY and ribosomal protein (rp) gene sequences provided additional molecular markers of the 'Ca. Phytoplasma pruni' lineage. We propose that the term 'Ca. Phytoplasma pruni' be applied to phytoplasma strains whose 16S rRNA gene sequences contain the oligonucleotide sequences of unique regions that are designated in the formally published description of the taxon. Such strains include X-disease phytoplasma and - within the tolerance of a single base difference in one unique sequence - peach rosette, peach red suture, and little peach phytoplasmas. Although not employed for taxon delineation in this work, we further propose that secY, rp, and other genetic loci from the reference strain of a taxon, and where possible oligonucleotide sequences of unique regions of those genes that distinguish taxa within a given 16Sr group, be incorporated in emended descriptions and as part of future descriptions of 'Candidatus Phytoplasma' taxa. C1 [Davis, Robert E.; Zhao, Yan; Dally, Ellen L.; Lee, Ing-Ming] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. [Jomantiene, Rasa] Nat Res Ctr, Phytovirus Lab, LT-08412 Vilnius, Lithuania. [Douglas, Sharon M.] Connecticut Agr Expt Stn, New Haven, CT 06511 USA. RP Davis, RE (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. EM robert.davis@ars.usda.gov NR 40 TC 35 Z9 36 U1 1 U2 17 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD FEB PY 2013 VL 63 BP 766 EP 776 DI 10.1099/ijs.0.041202-0 PN 2 PG 11 WC Microbiology SC Microbiology GA 120KY UT WOS:000317170400059 PM 22798643 ER PT J AU Peterson, SW Manitchotpisit, P Leathers, TD AF Peterson, Stephen W. Manitchotpisit, Pennapa Leathers, Timothy D. TI Aureobasidium thailandense sp nov isolated from leaves and wooden surfaces SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID DIVERSITY; SEQUENCE AB Aureobasidium thailandense sp. nov. is described from cultures of material collected on leaves and wooden surfaces in Thailand and the type isolate is NRRL 58539(T). Phylogenetically it is distinct from other species of the genus Aureobasidium. Phenotypically it is distinguished by its cardinal growth temperatures, salt tolerance and production of reddish brown hyphal pigmentation in PDA cultures, but micro-morphologically it is not clearly distinguishable from Aureobasidium pullulans. Unlike A. pullulans, A. thailandense sp. nov. produces a non-pullulan extracellular polysaccharide whose characteristics are unknown. The two known isolates of A. thailandense sp. nov. possess an approx. 500 bp type I intron in the 18S rRNA gene that is present in ITS amplifications using primers ITS4 and ITS5. A. pullulans isolates uniformly lack this intron. C1 [Peterson, Stephen W.] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Rangsit Univ, Fac Sci, Dept Med Sci, Biochem Unit, Lakhok, Patumthani, Thailand. [Leathers, Timothy D.] USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Prod Technol Res Unit, USDA, Peoria, IL 61604 USA. RP Peterson, SW (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Stephen.Peterson@ars.usda.gov FU Thailand Research Fund through the Royal Golden Jubilee (RGJ) PhD. Program [PHD/4.S.CU/48/Q.1] FX P. M. was supported in much of this work by a grant from the Thailand Research Fund through the Royal Golden Jubilee (RGJ) PhD. Program (Grant number PHD/4.S.CU/48/Q.1) and this support is gratefully acknowledged. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable. USDA is an equal opportunity provider and employer. NR 25 TC 6 Z9 6 U1 1 U2 9 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD FEB PY 2013 VL 63 BP 790 EP 795 DI 10.1099/ijs.0.047613-0 PN 2 PG 6 WC Microbiology SC Microbiology GA 120KY UT WOS:000317170400062 PM 23178722 ER PT J AU Etminan, M Bird, ST Delaney, JA Bressler, B Brophy, JM AF Etminan, Mahyar Bird, Steven T. Delaney, Joseph A. Bressler, Brian Brophy, James M. TI Isotretinoin and Risk for Inflammatory Bowel Disease A Nested Case-Control Study and Meta-analysis of Published and Unpublished Data SO JAMA DERMATOLOGY LA English DT Article ID CROHNS-DISEASE; ACNE AB Objective: To examine the association between isotretinoin and the risk for inflammatory bowel disease (IBD) among women of reproductive age. Design: Nested case-control study and meta-analysis. Setting: A US health claims database. Participants: We formed a cohort of women aged 18 to 46 years who had received at least 1 oral contraceptive prescription from May 1, 2001, through December 31, 2009. The IBD cases were required to have 3 health care contacts with documentation of IBD or a single health care contact followed by use of a drug to treat IBD. Twenty controls were selected for each case using incidence-density sampling, matched on age and date of diagnosis. Main Outcome Measures: Risk ratios (RRs) were formed for incident cases of IBD associated with the use of isotretinoin. A subgroup analysis examined the risk for IBD among those diagnosed as having Crohn disease (CD) and ulcerative colitis (UC). A meta-analysis of published and unpublished studies assessing isotretinoin and IBD used a random-effects model to estimate a pooled RR. Results: In the case-control study, we identified 2159 IBD cases (1056 with UC and 1103 with CD) and matched them with 43 180 controls. Only 10 cases (0.46%) and 191 controls (0.44%) were exposed to isotretinoin. The adjusted RR for IBD was 0.99 (95% CI, 0.52-1.90). The RRs for UC and CD were 1.10 (95% CI, 0.44-2.70) and 0.91 (0.37-2.25), respectively. For the meta-analysis, the pooled RR for IBD for the 5 studies was 0.94 (95% CI, 0.65-1.36). Conclusions: The results of this study do not suggest an increase in the risk for IBD, including UC or CD, with use of isotretinoin. Because inflammatory acne in children and adolescents carries a high psychological burden, clinicians should not be discouraged from prescribing this drug owing to a putative association with IBD. JAMA Dermatol. 2013;149(2):216-220 C1 [Etminan, Mahyar] Univ British Columbia, Therapeut Evaluat Unit, Child & Family Res Inst British Columbia, Vancouver, BC V5Z 4H4, Canada. [Bressler, Brian] Univ British Columbia, Div Gastroenterol, Vancouver, BC V5Z 4H4, Canada. [Etminan, Mahyar] Univ British Columbia, Fac Med, Dept Med, Vancouver, BC V5Z 4H4, Canada. [Bird, Steven T.] USDA, Off Management, Ctr Drug Evaluat & Res, Acad Collaborat Program, Silver Spring, MD USA. [Delaney, Joseph A.] Univ Washington, Sch Publ Hlth, Dept Epidemiol, Seattle, WA 98195 USA. [Brophy, James M.] McGill Univ, Dept Epidemiol Biostat & Med, Montreal, PQ, Canada. RP Etminan, M (reprint author), Univ British Columbia, Therapeut Evaluat Unit, Child & Family Res Inst British Columbia, 950 W 28th Ave,Room A4-195, Vancouver, BC V5Z 4H4, Canada. EM metminan@popi.ubc.ca NR 14 TC 27 Z9 29 U1 0 U2 8 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 2168-6068 J9 JAMA DERMATOL JI JAMA Dermatol. PD FEB PY 2013 VL 149 IS 2 BP 216 EP 220 DI 10.1001/jamadermatol.2013.1344 PG 5 WC Dermatology SC Dermatology GA 127CH UT WOS:000317672300018 PM 23426479 ER PT J AU Reardon, CL Strauss, SL Mazzola, M AF Reardon, C. L. Strauss, S. L. Mazzola, M. TI Changes in available nitrogen and nematode abundance in response to Brassica seed meal amendment of orchard soil SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Brassica seed meal; Nitrification; Nematode; Orchard soil; Nitric oxide; Mineralization ID APPLE REPLANT DISEASE; COMMUNITY STRUCTURE; RESIDENT BACTERIA; PYTHIUM SPP.; PCR PRIMERS; SUPPRESSION; PRODUCTS; DENITRIFICATION; MINERALIZATION; POPULATIONS AB A better understanding of the effects of Brassicaceae seed meals on resident soil microbial communities is necessary to predict the efficacy of these plant residues as either a biofumigant or organic fertilizer. This study analyzed the influence of high (Brassica juncea) and low (Brassica napus) glucosinolate content seed meals in addition to myrosinase-inactive derivatives on soil microbial community function with respect to nitrogen (N) cycling. All of the seed meal amendments stimulated nitric oxide (NO) generation in an orchard soil. N-mineralization occurred in response to B. juncea seed meal application but the amount of mineralization was reduced by the presence of active myrosinase and corresponding generation of allyl isothiocyanate. Microbial communities responded differentially to seed meal amendments: nematode abundance was enhanced by seed meals with either low glucosinolate or no myrosinase activity whereas fungal and bacterial abundance in soil did not exhibit significant changes in response to any seed meal amendment. In addition to changes in overall abundance, nematode diversity was also modified in response to seed meal amendment and differed among the amendments that enhanced nematode abundance. Collectively, these results indicate that microbial communities and overall soil function respond differentially to both seed meal type/glucosinolate content and isothiocyanate generation. These findings have significance for the efficient use of Brassicaceae residues as a source of plant available nitrogen. Published by Elsevier Ltd. C1 [Reardon, C. L.; Strauss, S. L.; Mazzola, M.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Mazzola, M (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM mark.mazzola@ars.usda.gov RI Strauss, Sarah/I-5141-2016 OI Strauss, Sarah/0000-0001-7494-5320 NR 52 TC 13 Z9 14 U1 8 U2 69 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2013 VL 57 BP 22 EP 29 DI 10.1016/j.soilbio.2012.10.011 PG 8 WC Soil Science SC Agriculture GA 121LU UT WOS:000317247100003 ER PT J AU Snyder, BA Callaham, MA Lowe, CN Hendrix, PF AF Snyder, Bruce A. Callaham, Mac A., Jr. Lowe, Christopher N. Hendrix, Paul F. TI Earthworm invasion in North America: Food resource competition affects native millipede survival and invasive earthworm reproduction SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Millipede; Earthworm; Sigmoria; Amynthas; Competition; Food preference; Invasive species ID TEMPERATE FORESTS; AMYNTHAS-AGRESTIS; MOUNTAINS; NITROGEN; CARBON; SOILS; USA AB The invasive non-native earthworm Amynthas agrestis (Goto and Hatai, 1899) has recently been documented invading forests of the Appalachian Mountains in the southeastern United States. This epigeic earthworm decreases the depth of organic soil horizons, and this may play a role in the decrease of millipede richness and abundance associated with A. agrestis invasion. To investigate the mechanisms behind these effects, A. agrestis and the millipede Sigmoria ainsliei (Xystodesmidae) were placed into microcosms with soil and either L horizon, F and H horizon, or a combination L/FH treatment. Microcosms were destructively sampled and reconstructed with the same treatments every four weeks to assess faunal fresh weight change and survival. Soils from earthworm treatments were wet-sieved for cocoons to assess treatment effects on reproduction. On average, millipede mortality occurred 88 days sooner in treatments that did not have FH horizon material, and within all litter treatments millipedes tended to survive longer when A. agrestis was absent. Earthworms maintained higher fresh weight in L/FH than FH or L treatments. With a single exception, no A. agrestis cocoons were recovered from microcosms that also contained S. ainsliei. The results suggest that A. agrestis and S. ainsliei may compete for food resources, particularly the smaller particle material in the FH horizons of the forest floor. Millipedes may exert some biotic resistance to A. agrestis invasion, as diminished earthworm fecundity was observed in experimental units containing both species. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Snyder, Bruce A.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Snyder, Bruce A.; Hendrix, Paul F.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Callaham, Mac A., Jr.] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, USDA, Athens, GA USA. [Lowe, Christopher N.] Univ Cent Lancashire, Sch Built & Nat Environm, Preston PR1 2HE, Lancs, England. [Hendrix, Paul F.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. RP Snyder, BA (reprint author), Kansas State Univ, Div Biol, 116 Ackert Hall, Manhattan, KS 66506 USA. EM bruceasnyder@gmail.com OI Lowe, Christopher/0000-0002-3893-7371 FU National Science Foundation [0236276]; Great Smoky Mountains National Park [GRSM-00337] FX The authors would like to acknowledge the assistance of L. Dame, B. Sheko, A. Silletti, S. C. Rostkowski, K. Seader, the University of Central Lancashire (Preston, UK), the USDA Forest Service, and the staff of the Great Smoky Mountains National Park (study number GRSM-00337) and Great Smoky Mountains Institute at Tremont. This study was supported in part by National Science Foundation grant number 0236276 to the University of Georgia Research Foundation, Inc. NR 19 TC 10 Z9 10 U1 5 U2 65 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2013 VL 57 BP 212 EP 216 DI 10.1016/j.soilbio.2012.08.022 PG 5 WC Soil Science SC Agriculture GA 121LU UT WOS:000317247100024 ER PT J AU Wang, P Kinraide, TB Smolders, E Zhou, DM Menzies, NW Thakali, S Xia, WW Hao, XZ Peijnenburg, WJGM Kopittke, PM AF Wang, Peng Kinraide, Thomas B. Smolders, Erik Zhou, Dong-Mei Menzies, Neal W. Thakali, Sagar Xia, Wei-Wei Hao, Xiu-Zhen Peijnenburg, Willie J. G. M. Kopittke, Peter M. TI An electrostatic model predicting Cu and Ni toxicity to microbial processes in soils SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Nickel; Copper; Potential nitrification rate; Glucose-induced respiration; Osmotic effect; Electrostatic toxicity model; Surface electrical potential; Zeta potential ID SURFACE ELECTRICAL POTENTIALS; COPPER-CONTAMINATED SOILS; PLANT-CELL MEMBRANES; BIOTIC LIGAND MODEL; ION CONCENTRATIONS; CHARGE DENSITY; HEAVY-METALS; BACTERIA; SPECIATION; TOLERANCE AB Toxicity data for microorganism in soil or in soil less cultures have been described with ion competition models, however these models disregard electrostatic and osmotic effects which are known to affect ion sorption and toxicity. Using European soils with diverse characteristics, the factors that influence the toxicity of soil Cu or Ni to potential nitrification rate (PNR) and glucose-induced respiration (GIR) were evaluated based on the electrical potential (Psi 0) and ion activities ({M2+}(0)) at the outer surfaces of bacterial cell membranes (CMs). The zeta potentials () of bacterial (Escherichia coli) protoplasts, as affected by the ionic composition of the solution, were measured and used to estimate the parameters of a Gouy Chapman Stern (GCS) model which was then used to compute;Po values. The V/0 values varied widely with soil type and increased markedly (became less negative) as metal salts were added. Computed 1,1/0 was then used to predict the surface ion activities from the soil solution composition. The toxicity data (both PNR and GIR) were statistically related to (i) surface activities of free metal ions ({M2+}(0)), (ii) the ameliorative effect of surface H+ activity ({H+}(0)), (iii) the Psi 0-influenced electrical driving force for cation uptake across CMs, and (iv) osmotic effects. This electrostatic model predicted the observed GIR and PNR with R-adj(2) > 0.816 for observed vs. predicted PNR and R-adj(2) > 0.861 for observed vs. predicted GIR. These predictions were generally better than those by previous models. The suggestion that metal toxicity in spiked soils is partly related to a spike-induced osmotic increase is corroborated by fitting the model to spiked soils that were or were not leached and aged to reduce the osmotic increase. The predicted soil EC50 values (in mg metal/kg soil) were within a factor of 2.5 for up to nineteen European soils with a wide range of properties. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Wang, Peng; Zhou, Dong-Mei; Xia, Wei-Wei; Hao, Xiu-Zhen] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [Wang, Peng; Menzies, Neal W.; Kopittke, Peter M.] Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld 4072, Australia. [Kinraide, Thomas B.] USDA, Agr Res Serv, Appalachian Farming Syst Res Ctr, Beaver, WV 25813 USA. [Smolders, Erik] Katholieke Univ Leuven, Div Soil & Water Management, B-3001 Heverlee, Belgium. [Thakali, Sagar] Gradient Corp, Cambridge, MA 02138 USA. [Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Lab Ecol Risk Assessment RIVM, NL-3720 BA Bilthoven, Netherlands. [Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, Dept Conservat Biol, NL-2300 RA Leiden, Netherlands. RP Zhou, DM (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. EM dmzhou@issas.ac.cn RI Kopittke, Peter/A-6026-2011; Peijnenburg, Willie/M-4110-2013; Wang, Peng/E-2008-2012; Menzies, Neal/L-1338-2013; OI Kopittke, Peter/0000-0003-4948-1880; Wang, Peng/0000-0001-8622-8767; Menzies, Neal/0000-0003-0207-070X; peijnenburg, willie/0000-0003-2958-9149 FU National Natural Science Foundation of China [31172034]; Chinese Academy of Sciences [KZCX2-YVV-Q0202] FX This work was supported financially by the National Natural Science Foundation of China (31172034) and the Knowledge Innovative Project of Chinese Academy of Sciences (KZCX2-YVV-Q0202). Statement of Equal Opportunity and Product Disclaimer: The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products does not imply recommendation or endorsement by the USDA. NR 48 TC 10 Z9 10 U1 2 U2 60 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2013 VL 57 BP 720 EP 730 DI 10.1016/j.soilbio.2012.09.002 PG 11 WC Soil Science SC Agriculture GA 121LU UT WOS:000317247100081 ER PT J AU Kelley, CJ Keller, CK Evans, RD Orr, CH Smith, JL Harlow, BA AF Kelley, Christopher J. Keller, C. Kent Evans, R. D. Orr, C. H. Smith, Jeffrey L. Harlow, Benjamin A. TI Nitrate-nitrogen and oxygen isotope ratios for identification of nitrate sources and dominant nitrogen cycle processes in a tile-drained dryland agricultural field SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Nitrification; Nitrate-nitrogen and oxygen isotope ratios; Nitrogen cycle; Nitrate leaching; Nitrate fluxes ID FRESH-WATER; NITROBACTER-AGILIS; RIPARIAN AREA; SOIL; DENITRIFICATION; GROUNDWATER; DELTA-N-15; FERTILIZER; BUDGET; WHEAT AB Agricultural systems are a leading source of reactive nitrogen to aquatic and atmospheric ecosystems. In this study environmental delta N-15(nitrate) and delta O-18(nitrate) are used to identify the dominant nitrogen cycle processes and sources of NOT leached from a tile-drained, dryland agricultural field. Tile-drain water discharge delta O-18(nitrate) values suggest nitrification is the dominant soil nitrogen cycle process throughout the 5-year study period, because the expected 5180nitrate from nitrification is indistinguishable from the measured value of 1.3 +/- 1.5%. Given this there is no evidence that denitrification was occurring at a large enough scale to influence [NO (3) over bar]. Values for delta N-15(nitrate) varied seasonally during the high-discharge season (January through May) and low-discharge season (June through December) with weighted means of 1.0 +/- 1.0%. and 4.7 +/- 2.3% respectively. This suggests that during the high-discharge season NO originates from nitrification of NH4+ fertilizer, and during the low-discharge season NO (3) over bar originates from mineralized soil organic nitrogen. The estimated travel time through the soil for nitrified NH4+ fertilizer leached during the high-discharge season is less than 6 months, from fall fertilizer application to leaching through the tile-drain. This study suggests that understanding the hydrology of a region is necessary before dominant nitrogen cycling processes can be reliably determined. 2012 Elsevier Ltd. All rights reserved. C1 [Kelley, Christopher J.; Keller, C. Kent; Orr, C. H.] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. [Evans, R. D.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Smith, Jeffrey L.] Washington State Univ, USDA ARS, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Evans, R. D.; Harlow, Benjamin A.] Washington State Univ, Stable Isotope Core Lab, Pullman, WA 99164 USA. RP Kelley, CJ (reprint author), Washington State Univ, Sch Environm, POB 642818, Pullman, WA 99164 USA. EM christopher.kelley@email.wsu.edu FU NSPIRE; Washington State University School of the Environment; Department of Crop and Soil Science FX We thank the three anonymous reviewers for their critical comments on earlier versions, especially the second reviewer who provided through and well thought-out comments on two earlier versions of this manuscript. We also thank the NSPIRE fellowship program at Washington State University, Washington State University School of the Environment and Department of Crop and Soil Science, all collaborators and staff, especially the previous graduate students who have contributed to building the hydrology data set, at the Cook Agronomy Farm. NR 44 TC 5 Z9 7 U1 3 U2 43 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2013 VL 57 BP 731 EP 738 DI 10.1016/j.soilbio.2012.10.017 PG 8 WC Soil Science SC Agriculture GA 121LU UT WOS:000317247100082 ER PT J AU Kojima, Y Heitman, JL Flerchinger, GN Horton, R AF Kojima, Yuki Heitman, Joshua L. Flerchinger, Gerald N. Horton, Robert TI Numerical Evaluation of a Sensible Heat Balance Method to Determine Rates of Soil Freezing and Thawing SO VADOSE ZONE JOURNAL LA English DT Article ID TIME-DOMAIN REFLECTOMETRY; UNFROZEN WATER-CONTENT; SEASONALLY FROZEN SOILS; THERMAL-PROPERTIES; UNSATURATED SOIL; POROUS-MEDIA; LIQUID WATER; PULSE METHOD; ICE CONTENT; MOVEMENT AB In situ determination of soil freezing and thawing is difficult despite its importance for many environmental processes. A sensible heat balance (SHB) method using a sequence of heat pulse probes has been shown to accurately measure water evaporation in subsurface soil, and it has the potential to measure soil freezing and thawing. Determination of soil freezing and thawing may be more challenging than evaporation, however, because the latent heat of fusion is smaller than the latent heat of vaporization. Furthermore, convective heat flow associated with liquid water flow and occurrence of evaporation or condensation during freezing and thawing may cause inaccurate estimation of freezing and thawing with the SHB method. The objective of this study was to examine the applicability of the SHB concept to soil freezing and thawing. Soil freezing and thawing events were simulated with the simultaneous heat and water (SHAW) model. Ice contents were estimated by applying the SHB concept to numerical data produced by the SHAW model. Close agreement between the SHB-estimated and the SHAW-simulated ice contents were observed at depths below 24 mm. The main cause of inaccuracies with the SHB method was poor estimation of heat conduction at the 12-mm depth, possibly due to simplifications of temporal or vertical distributions of temperature and thermal conductivity. The effects of convective heat flow and concurrent evaporation or condensation and freezing or thawing on the SHB method were small. The results indicate that the SHB method is conceptually suitable for estimating soil freezing and thawing. Independent, accurate estimates of thermal properties must be available to effectively use the SHB method to determine in situ soil freezing and thawing. C1 [Kojima, Yuki; Horton, Robert] Iowa State Univ, Agron Dep, Ames, IA 50011 USA. [Heitman, Joshua L.] N Carolina State Univ, Soil Sci Dep, Raleigh, NC 27695 USA. [Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res, Boise, ID 83712 USA. RP Kojima, Y (reprint author), Iowa State Univ, Agron Dep, Ames, IA 50011 USA. EM ykojima@iastate.edu FU National Science Foundation [0809656, 1215864]; Hatch Act, State of Iowa; State of North Carolina funds FX This work was supported by the National Science Foundation under Grants 0809656 and 1215864 and by the Hatch Act, State of Iowa, and State of North Carolina funds. We acknowledge Dr. Robert Ewing, Iowa State University, for helpful discussions. NR 54 TC 5 Z9 5 U1 4 U2 41 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD FEB PY 2013 VL 12 IS 1 DI 10.2136/vzj2012.0053 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 127QK UT WOS:000317713300013 ER PT J AU Watanabe, K Kito, T Dun, S Wu, JQ Greer, RC Flury, M AF Watanabe, Kunio Kito, Tetsuya Dun, Shuhui Wu, Joan Q. Greer, R. Cory Flury, Markus TI Water Infiltration into a Frozen Soil with Simultaneous Melting of the Frozen Layer SO VADOSE ZONE JOURNAL LA English DT Article ID SNOWMELT INFILTRATION; FLOW; COVER; SIMULATION; TRANSPORT; MOVEMENT; RUNOFF; MODEL; JAPAN AB Understanding water infiltration into frozen soil is important for preventing soil erosion and managing soil water and nutrients. In this study, we performed a column experiment on infiltration through frozen soil using a variably-saturated silt loam. Three soil columns (7.8 cm id., 35 cm long), with three different initial soil water contents, were cooled from the top to form a frozen layer of the same thickness. The columns were instrumented with 34 thermocouples, seven time-domain reflectometry (TDR) probes, and seven tensiometers. Water at a temperature of 3.5 degrees C was applied to the top of the columns with a 15-cm constant head. We monitored ice and liquid water contents, temperatures, and the position of the infiltration front. Three phases of infiltration were observed: (i) no infiltration at the beginning, (ii) slow infiltration as the infiltration front advanced through the frozen layer, and (iii) increased infiltration as the infiltration front advanced through the unfrozen soil below the frozen layer. The duration of each phase became longer with increasing initial soil water content as the infiltration rate of each phase decreased. The volumetric ice content and thickness of the frozen layer controlled the infiltration process. We use a capillary bundle model to characterize the hydraulic conductivity as a function of ice content during infiltration. Based on our experimental data and results, we mechanistically describe the water infiltration into frozen soil. C1 [Watanabe, Kunio; Kito, Tetsuya] Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan. [Dun, Shuhui; Wu, Joan Q.] Washington State Univ, Dept Biol Syst Engn, Puyallup, WA 98371 USA. [Greer, R. Cory] USDA NRCS, Emmett, ID 83617 USA. [Flury, Markus] Washington State Univ, Dept Crop & Soil Sci, Puyallup, WA 98371 USA. RP Watanabe, K (reprint author), Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan. EM kunio@bio.mie-u.ac.jp RI Flury, Markus/H-2983-2012 OI Flury, Markus/0000-0002-3344-3962 FU Japanese Ministry of Education, Science, Sports and Culture [23580328]; Washington State University (WSU), Pullman, Washington, USA; Inland Northwest Research Alliance FX This research was in part funded by the Japanese Ministry of Education, Science, Sports and Culture, Grant-in-Aids for Scientific Research (C) 23580328. We thank Y. Iwata (National Agricultural Research Center for Hokkaido Region, Japan) for providing the soil samples. The preliminary experiments were carried out at Washington State University (WSU), Pullman, Washington, USA with funding support from the Inland Northwest Research Alliance. We acknowledge Donald K. McCool (Retired, USDA-ARS Land Management and Water Conservation Research Unit), Mitchell A. Plummer (Idaho National Laboratory, US Department of Energy), and Jon Mathison, Scott D. Mattison and Wayne DeWitt (WSU) for help in the preliminary experiments. We thank the anonymous reviewers for their comments which helped to improve the manuscript. NR 35 TC 6 Z9 6 U1 3 U2 54 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD FEB PY 2013 VL 12 IS 1 DI 10.2136/vzj2011.0188 PG 7 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 127QK UT WOS:000317713300005 ER PT J AU Reichmann, LG Sala, OE Peters, DPC AF Reichmann, Lara G. Sala, Osvaldo E. Peters, Debra P. C. TI Precipitation legacies in desert grassland primary production occur through previous-year tiller density SO ECOLOGY LA English DT Article DE aboveground net primary production; Chihuahuan Desert; desert grasslands; precipitation legacies; rainfall manipulation; tiller dynamics ID ALTERED RAINFALL PATTERNS; SEMIARID ECOSYSTEMS; CHIHUAHUAN DESERT; PATAGONIAN STEPPE; SHORTGRASS STEPPE; SOIL-WATER; ABOVEGROUND BIOMASS; BOUTELOUA-GRACILIS; PERENNIAL GRASSES; TALLGRASS PRAIRIE AB In arid ecosystems, current-year precipitation often explains only a small proportion of annual aboveground net primary production (ANPP). We hypothesized that lags in the response of ecosystems to changes in water availability explain this low explanatory power, and that lags result from legacies from transitions from dry to wet years or the reverse. We explored five hypotheses regarding the magnitude of legacies, two possible mechanisms, and the differential effect of previous dry or wet years on the legacy magnitude. We used a three-year manipulative experiment with five levels of rainfall in the first two years (-80% and -50% reduced annual precipitation (PPT), ambient, +50% and +80% increased PPT), and reversed treatments in year 3. Legacies of previous two years, which were dry or wet, accounted for a large fraction (20%) of interannual variability in production on year 3. Legacies in ANPP were similar in absolute value for both types of precipitation transitions, and their magnitude was a function of the difference between previous and current-year precipitation. Tiller density accounted for 40% of legacy variability, while nitrogen and carry-over water availability showed no effect. Understanding responses to changes in interannual precipitation will assist in assessing ecosystem responses to climate change-induced increases in precipitation variability. C1 [Reichmann, Lara G.; Sala, Osvaldo E.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Reichmann, Lara G.; Sala, Osvaldo E.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Reichmann, Lara G.; Sala, Osvaldo E.; Peters, Debra P. C.] New Mexico State Univ, Jornada Basin Long Term Ecol Res Program, Las Cruces, NM 88003 USA. [Peters, Debra P. C.] New Mexico State Univ, ARS, USDA, Jornada Expt Range, Las Cruces, NM 88003 USA. RP Reichmann, LG (reprint author), ARS, USDA, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM Lara.Reichmann@austin.utexas.edu RI Young, Kristina/M-3069-2014 FU National Science Foundation [DEB 09-17668, DDIG 0910027, DEB 06-18210] FX We thank L. Gherardi, G. A. Gil, D. D. Correa, E. Johnson, J. P. Midez, O. O'Brien, F. Spirito, A. Asay, J. H. Angell III, M. Fuchs, and S. B. Easter for invaluable assistance in the field and in the lab, and the Jornada LTER and JER-USDA personnel for their logistical support. This work was supported by National Science Foundation grants DEB 09-17668 and DDIG 0910027 to Arizona State University, and DEB 06-18210 to New Mexico State University as part of the Jornada Basin LTER. NR 56 TC 45 Z9 47 U1 11 U2 130 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD FEB PY 2013 VL 94 IS 2 BP 435 EP 443 PG 9 WC Ecology SC Environmental Sciences & Ecology GA 117DQ UT WOS:000316932800019 PM 23691662 ER PT J AU Gigot, JA Zasada, IA Walters, TW AF Gigot, Jessica A. Zasada, Inga A. Walters, Thomas W. TI Integration of brassicaceous seed meals into red raspberry production systems SO APPLIED SOIL ECOLOGY LA English DT Article DE Solarization; Seed meal; Raspberry; Phytophthora; Pratylenchus; Brassica ID APPLE REPLANT DISEASE; SOIL SOLARIZATION; ROOT-ROT; NEMATODE COMMUNITIES; METHYL-BROMIDE; FOOD-WEB; EFFICACY; AMENDMENTS; BIOFUMIGATION; SUPPRESSION AB Brassicaceous seed meals are an alternative to synthetic chemical fumigation for the pre-plant management of soil borne organisms. Greenhouse, microplot, and field experiments were conducted to evaluate the effect of Brassica juncea and Sinapis alba seed meals against the oomycete pathogen Phytophthora rubi and the plant-parasitic nematode Pratylenchus penetrans, both chronic problems in red raspberry (Rubus idaeus) production systems. Phytotoxicity to raspberry, as well as the effect of brassicaceous seed meals on non-target soil organisms was also investigated. In greenhouse studies, P. rubi and P. penetrans were suppressed by B. juncea and S. alba seed meals at rates of 0.5 and 1:0% (v v(-1)), respectively. In field experiments using buried bags of P. rubi inoculum, suppression of P. rubi by B. juncea and S. alba seed meals was enhanced with solarization, however only to a depth of 15 cm and only when target heat units in soil (222 h > 29 degrees C) accumulated. In microplot studies, soils from two different raspberry fields were amended with B. juncea at 0.5% or S. alba at 1.0% (v v(-1)) seed meals; nematode community abundance and composition were evaluated over time. Both seed meal type and soil affected nematode community abundance and composition. B. juncea seed meal eliminated all nematodes, including P. penetrans, in both soils one week after application and this seed meal increased the overall enrichment index (El) at five weeks. S. alba seed meal reduced nematode population densities at one week after application, and increased the structure index (SI) at five weeks. In a greenhouse trial, phytotoxicity (>40% plant damage) to raspberry 'Meeker' was observed one week after the application of S. alba seed meal at 1.0% (v v(-1)) only and did not extend beyond one week. A combination of field and greenhouse experiments demonstrated that brassicaceous seed meals have the potential to effectively reduce populations of P. rubi and P. penetrans, two important pests of raspberry. However, inconsistent results in field trials demonstrate the need to further explore the use of brassicaceous seed meals, alone or in combination with other management practice for the suppression of the soil borne organisms. (C) 2012 Elsevier B.V. All rights reserved. C1 [Gigot, Jessica A.] Northwest Indian Coll Swinomish, La Conner, WA 98257 USA. [Zasada, Inga A.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Walters, Thomas W.] Washington State Univ, Northwestern Washington Res & Educ Ctr, Mt Vernon, WA 98273 USA. RP Gigot, JA (reprint author), Northwest Indian Coll Swinomish, La Conner, WA 98257 USA. EM jgigot@nwic.edu NR 45 TC 5 Z9 6 U1 0 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2013 VL 64 BP 23 EP 31 DI 10.1016/j.apsoil.2012.10.013 PG 9 WC Soil Science SC Agriculture GA 113ZD UT WOS:000316707800004 ER PT J AU Lehman, RM Garland, JL Osborne, SL AF Lehman, R. Michael Garland, Jay L. Osborne, Shannon L. TI Applying an oxygen-based respiratory assay to assess soil microbial responses to substrate and N availability SO APPLIED SOIL ECOLOGY LA English DT Article DE BD oxygen biosensor system; Bioavailable N; Community-level physiological profiling (CLPP); Soil respiration ID CARBON-SOURCE UTILIZATION; MICROTITER PLATE; COMMUNITIES; DIVERSITY; MANAGEMENT; PROFILES; BIOLOG AB Documented approaches for measuring soil microbial activities and their controlling factors under field conditions are needed to advance understanding of soil microbial processes for numerous applications. We manipulated field plots with carbon (C) and nitrogen (N) additions to test the capability of a respiratory assay to: (1) measure respiration of endogenous soil C in comparison to field-measured CO2 fluxes; (2) determine substrate-induced respiratory (SIR) activities that are consistent with substrate availability in the field; and, (3) report N availability in the field based on assay responses with and without added N. The respiratory assay utilizes a microplate containing an oxygen-sensitive fluorescent ruthenium dye. Respiratory activities measured with this approach have previously been shown to occur within short (6-8 h) incubation periods using low substrate concentrations that minimize enrichment during the assay. Field treatments were conducted in a randomized full-factorial design with C substrate (casamino acids, glucose, or none) and inorganic N (+/-) as the treatment factors. With one exception, we found that respiration of endogenous soil C in the assay responded to the field treatments in a similar manner to CO2 fluxes measured in the field. Patterns of SIR with low concentrations of added amino acid or carbohydrate substrate (200 mu g C g(-1) soil) were consistent with field treatments. The ratio (N-ratio) of carbohydrate respiration with added N (25 mu g N g(-1) soil) to the same without N in the assay was significantly (P < 0.05) decreased by field N amendment. The carbohydrate N-ratio exhibited a logarithmic relationship (r = 0.64, P < 0.05) with extractable inorganic soil nitrate and ammonium concentrations. These data significantly extend and support the capability of this oxygen-based respiratory assay to evaluate in situ soil activities and examine factors that limit these activities. Published by Elsevier B.V. C1 [Lehman, R. Michael; Osborne, Shannon L.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Garland, Jay L.] Dynamac Corp, Kennedy Space Ctr, FL 32899 USA. RP Lehman, RM (reprint author), North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM michael.lehman@ars.usda.gov OI Lehman, Michael/0000-0002-3391-3178 FU USDA-NRI [2006-35107-17226] FX This research was supported by USDA-NRI Soil Processes Grant #2006-35107-17226. Technical assistance in the field or laboratory was provided by USDA-ARS employees Amy Christie, Kurt Dagel, Ann Qualm, and Darci Borkowski.Disclaimer: "Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer." NR 25 TC 3 Z9 3 U1 0 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2013 VL 64 BP 127 EP 134 DI 10.1016/j.apsoil.2012.11.006 PG 8 WC Soil Science SC Agriculture GA 113ZD UT WOS:000316707800017 ER PT J AU Mothapo, NV Grossman, JM Maul, JE Shi, W Isleib, T AF Mothapo, N. V. Grossman, J. M. Maul, J. E. Shi, W. Isleib, T. TI Genetic diversity of resident soil rhizobia isolated from nodules of distinct hairy vetch (Vicia villosa Roth) genotypes SO APPLIED SOIL ECOLOGY LA English DT Article DE Genetic diversity; Cropping history; Rhizobium leguminosarum by. viciae; Hairy vetch; BOX-PCR ID LEGUMINOSARUM BIOVAR VICIAE; PHASEOLUS-VULGARIS L.; EASTERN CANADIAN PRAIRIE; BV VICIAE; BRADYRHIZOBIUM-JAPONICUM; SYMBIOTIC EFFICIENCY; NATURAL-POPULATIONS; CULTIVATED SOILS; N-2 FIXATION; COVER CROP AB Hairy vetch (Vicia villosa Roth, HV) is widely grown as a legume cover crop throughout the U.S.A., with biological nitrogen fixation (BNF) through symbiosis with Rhizobium leguminosarum biovar viciae (RIv) being one of the most sought after benefits of its cultivation. This study determined if HV cultivation history and plant genotype affect genetic diversity of resident RIv. Soil samples were collected from within farmers' fields at Graham, Cedar Grove and Ivanhoe sites in North Carolina and pairs of genetically similar hairy vetch genotypes used as trap hosts. A total of 519 Rlv strains were isolated from six paired field soils, three with and three without histories of HV cultivation. A total of 46 strains failed to PCR-amplify the nifH gene; however nodC PCR amplification of these nifH-negative strains resulted in amplification of 22 of the strains. Repetitive element polymerase chain reaction (rep-PCR) with BOX-Al R primer and redundancy analysis showed rhizobial diversity to vary greatly within and between fields, with over 30 BOX banding patterns obtained across the six fields. Cluster analysis of BOX-PCR banding patterns resulted in 36 genetic groups of Rlv at a similarity level of 70%, with 15 of the isolates from fields with HV history not belonging to any of the clusters. Site was found to be the main driver of isolate diversity overall, explaining 57%, of the total variation among rhizobia occupying HV nodules, followed by history of hairy vetch cultivation. Evidence of a HV host genotype influence on the populations of rhizobia that infect hairy vetch was also observed, with plant genotype explaining 12.7% of the variation among all isolates. Our results show that second to site, HV cultivation history was the most important driver of rhizobial nodule community structure and increases the genetic diversity of resident Rlv in soils. (C) 2012 Elsevier B.V. All rights reserved. C1 [Mothapo, N. V.; Grossman, J. M.; Shi, W.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Maul, J. E.] USDA ARS Sustainable Agr Syst Lab, Beltsville, MD USA. [Isleib, T.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Grossman, JM (reprint author), N Carolina State Univ, Dept Soil Sci, 101 Derieux St,4242 Williams Hall,Box 7619, Raleigh, NC 27695 USA. EM julie_grossman@ncsu.edu OI Grossman, Julie M/0000-0001-7341-9601 FU Fulbright Scholarship; USDA-SARE FX Farmers, Alex Hitt, Ken Dawson, Michael Fortune, Michael Porterfield and Stephan Hartman, are appreciated for making their fields available and willingly sharing their farm management information to help with the project. Funding support from a Fulbright Scholarship and the USDA-SARE program is greatly appreciated. NR 46 TC 5 Z9 7 U1 3 U2 69 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2013 VL 64 BP 201 EP 213 DI 10.1016/j.apsoil.2012.12.010 PG 13 WC Soil Science SC Agriculture GA 113ZD UT WOS:000316707800026 ER PT J AU Tian, G Franzluebbers, AJ Granato, TC Cox, AE O'Connor, C AF Tian, G. Franzluebbers, A. J. Granato, T. C. Cox, A. E. O'Connor, C. TI Stability of soil organic matter under long-term biosolids application SO APPLIED SOIL ECOLOGY LA English DT Article DE Biosolids; Soil organic C and N fractions; mineralization; US Midwest ID CARBON; STABILIZATION; AGGREGATION; NITROGEN AB Little is know on the impact of biosolids application on soil organic matter (SUM) stability, which contributes to soil C sequestration. Soil samples were collected in 2006 at plow layer from fields that received liquid and dry municipal biosolids application from 1972 to 2004 at the cumulative rate of 1416 Mg ha(-1) in mined soil and 1072 Mg ha(-1) in nonmined soil and control fields that received chemical fertilizer at Fulton County, western Illinois. The biosolids application increased the soil microbial biomass C (SMBC) by 5-fold in mined soil and 4-fold in nonmined soil. The biosolids-amended soils showed a high amount of basal respiration and N mineralization, but low metabolic quotient, and low rate of organic C and organic N mineralization. There was a remarkable increase in mineral-associated organic C from 6.9 g kg(-1) (fertilizer control) to 26.6 g kg(-1) (biosolids-amended) in mined soil and from 8.9 g kg(-1) (fertilizer control) to 23.1 g kg(-1) (biosolids-amended) in nonmined soil. The amorphous Fe and Al, which can improve SUM stability, were increased by 2-7 folds by the long-term biosolids application. It is evident from this study that the biosolids-modified SUM resists to decomposition more than that in the fertilizer treatment, thus long-term biosolids application could increase SUM stability. Published by Elsevier B.V. C1 [Tian, G.; Granato, T. C.; Cox, A. E.; O'Connor, C.] Metropolitan Water Reclamat Dist Greater Chicago, Environm Monitoring & Res Div, Monitoring & Res Dept, Chicago, IL USA. [Franzluebbers, A. J.] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. RP Tian, G (reprint author), Lue Hing R&D Complex,6001 W Pershing Rd, Cicero, IL 60804 USA. EM guanglong.tian@mwrd.org NR 29 TC 4 Z9 4 U1 0 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2013 VL 64 BP 223 EP 227 DI 10.1016/j.apsoi1.2012.12.001 PG 5 WC Soil Science SC Agriculture GA 113ZD UT WOS:000316707800028 ER PT J AU Nuttle, T Royo, AA Adams, MB Carson, WP AF Nuttle, Tim Royo, Alejandro A. Adams, Mary Beth Carson, Walter P. TI Historic disturbance regimes promote tree diversity only under low browsing regimes in eastern deciduous forest SO ECOLOGICAL MONOGRAPHS LA English DT Article DE browsing; canopy gaps; eastern deciduous forest, USA; factorial experiment; fire; forest diversity; Odocoileus virginianus; regeneration; succession; white-tailed deer ID BEECH BARK DISEASE; WHITE-TAILED DEER; CENTRAL HARDWOOD FOREST; MIXED-OAK FORESTS; UNITED-STATES; NORTH-AMERICA; PLANT-COMMUNITIES; GAP DYNAMICS; GROWTH; FIRE AB Eastern deciduous forests are changing in species composition and diversity outside of classical successional trajectories. Three disturbance mechanisms appear central to this phenomenon: fire frequency is reduced, canopy gaps are smaller, and browsers are more abundant. Which factor is most responsible is a matter of great debate and remains unclear, at least partly because few studies have simultaneously investigated more than one process. We conducted a large-scale experiment in mesophytic forests of West Virginia, USA, to test three key hypotheses: (1) the fire hypothesis (fire suppression limits diversity to few shade-tolerant, fire-intolerant species that replace and suppress many fire-tolerant species); (2) the gap hypothesis (small gaps typical of today's forests promote dominance of a few shade-tolerant species); and (3) the browsing hypothesis (overbrowsing by deer limits diversity to a few unpalatable species). We tested these hypotheses using a factorial experiment that manipulated surface fire, large canopy gap formation (gap size similar to 255 m(2)), and browsing by deer, and we followed the fates of >28 000 seedlings and saplings for five years. Understory tree communities in control plots were dominated (up to 90%) by Fagus grandifolia, averaging little more than two species, whereas overstories were diverse, with 10-15 species. Fire, large canopy gaps, and browsing all dramatically affected understory composition. However, our findings challenge views that fire and large canopy gaps can maintain or promote diversity, because browsers reduced the benefits of gaps and created depauperate understories following fire. Consequently, two major disturbances that once promoted tree diversity no longer do so because of browsing. Our findings appear to reconcile equivocal views on the role of fire and gaps. If browsers are abundant, these two disturbances either depress diversity or are less effective. Alternatively, with browsers absent, these disturbances promote diversity (three-to fivefold). Our results apply to large portions of eastern North America where deer are overabundant, and we provide compelling experimental evidence that historical disturbance regimes in combination with low browsing regimes typical of pre-European settlement forests could maintain high tree species diversity. However, restoring disturbances without controlling browsing may be counterproductive. C1 [Nuttle, Tim] Indiana Univ Penn, Dept Biol, Indiana, PA 15715 USA. [Royo, Alejandro A.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. [Adams, Mary Beth] US Forest Serv, USDA, No Res Stn, Parsons, WV 26287 USA. [Carson, Walter P.] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA. RP Nuttle, T (reprint author), Civil & Environm Consultants Inc, Ecol Serv, 333 Baldwin Rd, Pittsburgh, PA 15205 USA. EM tnuttle@cecinc.com FU USDA National Research Initiative Competitive Grant [99-35101-7732]; MeadWestvaco Corporation; USDA Forest Service Northern Experiment Station FX This work was supported by the USDA National Research Initiative Competitive Grant 99-35101-7732 to W. P. Carson, MeadWestvaco Corporation, and the USDA Forest Service Northern Experiment Station. We thank R. Collins for setting up the experiment and providing helpful advice, Q. Wu of the Statistics Consulting Service at the University of Pittsburgh for advice on statistical analysis, and A. Baumert, J. Paul, T. Pendergast IV, C. Peterson, H. Schumacher, S. Tonsor, and D. Yarnot for helpful comments on the manuscript. NR 76 TC 37 Z9 38 U1 7 U2 144 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9615 EI 1557-7015 J9 ECOL MONOGR JI Ecol. Monogr. PD FEB PY 2013 VL 83 IS 1 BP 3 EP 17 DI 10.1890/11-2263.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA 118RM UT WOS:000317043500002 ER PT J AU Kashian, DM Romme, WH Tinker, DB Turner, MG Ryan, MG AF Kashian, Daniel M. Romme, William H. Tinker, Daniel B. Turner, Monica G. Ryan, Michael G. TI Postfire changes in forest carbon storage over a 300-year chronosequence of Pinus contorta-dominated forests SO ECOLOGICAL MONOGRAPHS LA English DT Article DE carbon; chronosequence; lodgepole pine; net ecosystem carbon balance; net ecosystem production; Pinus contorta var. latifolia; postfire succession; Yellowstone National Park, Wyoming, USA ID YELLOWSTONE-NATIONAL-PARK; NET PRIMARY PRODUCTION; COARSE WOODY DEBRIS; AMERICAN BOREAL FORESTS; AGE-RELATED-CHANGES; OLD-GROWTH FORESTS; LODGEPOLE PINE; CLIMATE-CHANGE; LEAF-AREA; BLACK SPRUCE AB A warming climate may increase the frequency and severity of stand-replacing wildfires, reducing carbon (C) storage in forest ecosystems. Understanding the variability of postfire C cycling on heterogeneous landscapes is critical for predicting changes in C storage with more frequent disturbance. We measured C pools and fluxes for 77 lodgepole pine (Pinus contorta Dougl. ex Loud var. latifolia Engelm.) stands in and around Yellowstone National Park (YNP) along a 300-year chronosequence to examine how quickly forest C pools recover after a stand-replacing fire, their variability through time across a complex landscape, and the role of stand structure in this variability. Carbon accumulation after fire was rapid relative to the historical mean fire interval of 150-300 years, recovering nearly 80% of prefire C in 50 years and 90% within 100 years. Net ecosystem carbon balance (NECB) declined monotonically, from 160 g C.m(-2).yr(-1) at age 12 to 5 g C.m(-2).yr(-1) at age 250, but was never negative after disturbance. Decomposition and accumulation of dead wood contributed little to NECB relative to live biomass in this system. Aboveground net primary productivity was correlated with leaf area for all stands, and the decline in aboveground net primary productivity with forest age was related to a decline in both leaf area and growth efficiency. Forest structure was an important driver of ecosystem C, with ecosystem C, live biomass C, and organic soil C varying with basal area or tree density in addition to forest age. Rather than identifying a single chronosequence, we found high variability in many components of ecosystem C stocks through time; a >50% random subsample of the sampled stands was necessary to reliably estimate the nonlinear equation coefficients for ecosystem C. At the spatial scale of YNP, this variability suggests that landscape C develops via many pathways over decades and centuries, with prior stand structure, regeneration, and within-stand disturbance all important. With fire rotation projected to be <30 years by mid century in response to a changing climate, forests in YNP will store substantially less C (at least 4.8 kg C/m(2) or 30% less). C1 [Kashian, Daniel M.] Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA. [Romme, William H.] Colorado State Univ, Warner Coll Nat Resources, Ft Collins, CO 80523 USA. [Romme, William H.; Ryan, Michael G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Tinker, Daniel B.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Tinker, Daniel B.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Turner, Monica G.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Kashian, DM (reprint author), Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA. EM dkash@wayne.edu RI Ryan, Michael/A-9805-2008; Turner, Monica/B-2099-2010; Romme, William/C-7317-2016 OI Ryan, Michael/0000-0002-2500-6738; FU Joint Fire Sciences Program [03-1-1-06]; Wayne State University FX We thank Creighton Litton for access to his data describing recently burned stands within the study area and Scott Baggett for help with statistical analysis. Data collection and analyses would not have been possible without monumental efforts by our field assistants, particularly Kellen Nelson, who was involved in the field work in every field season associated with this project and supervised undergraduates in the field and the laboratory; we also are grateful to Heather Lyons who was instrumental in leading the field crews in multiple years. The sampling for and development of the allometric equations were based heavily upon the work of Rick Arcano and his Master's thesis at the University of Wyoming. We thank three separate field crews, including Therese Tepe, Lauren Alleman, Rick Arcano, Caitlin Balch-Burnett, Megan Busick, Lena Cicchelli, Brandon Corcoran, Janice Corcoran, Guadalupe Cummins, Bill Dodge, Lance East, Lance Farman, Deborah Fritts, Lisa Huttinger, Monique LaPerrierre, John Niedermiller, Kevin Ruzicka, and Andy Whelan. Laboratory analyses were supervised by Kellen Nelson and completed by Lauren Alleman, Marc Barker, Becky Dritz, Todd Hagadone, Tiann Heit, Chris Herron, Aliy Louie, Chris Martin, Tiffany Minton, Kevin Ruzicka, and Ashlee Wallin. We are indebted to Christie Hendrix, Stacey Gunther, Roy Renkin, and others at Yellow-stone National Park as well as Henry Harlow and Rich Viola at the University of Wyoming-National Park Service Research Center for their respective support in permitting, site access, and other logistical support. Comments and discussions with Dan Binkley greatly improved the initial draft of the manuscript. The majority of this study was funded by the Joint Fire Sciences Program (Project Number 03-1-1-06) and in part by Wayne State University. NR 118 TC 33 Z9 34 U1 14 U2 99 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9615 EI 1557-7015 J9 ECOL MONOGR JI Ecol. Monogr. PD FEB PY 2013 VL 83 IS 1 BP 49 EP 66 DI 10.1890/11-1454.1 PG 18 WC Ecology SC Environmental Sciences & Ecology GA 118RM UT WOS:000317043500004 ER PT J AU McRoberts, RE Naesset, E Gobakken, T AF McRoberts, Ronald E. Naesset, Erik Gobakken, Terje TI Accuracy and Precision for Remote Sensing Applications of Nonlinear Model-Based Inference SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Landsat; lidar; variable selection ID LIDAR SAMPLE SURVEY; REGRESSION MODELS; AIRBORNE LASER; HEDMARK COUNTY; FOREST AREA; BIOMASS; NORWAY AB In a forest inventory context, estimation for small areas and for remote and inaccessible regions may be problematic using traditional probability-or design-based inference because acquisition of sufficiently large samples to satisfy precision requirements is financially and/or logistically difficult. These problems can often be partially alleviated for inventory applications by enhancing inferences using models and remotely sensed independent variables. However, estimates obtained using probability-based, model-assisted estimators may still suffer detrimental effects as the result of small sample sizes. Model-based inference has the potential to alleviate these problems because precision is affected by other factors such as model specification. Nevertheless, model specification in the form of selection of independent variables often focuses exclusively on quality of fit with little consideration given to the precision of estimates of areal population parameters. Model-based inference is illustrated for two forest inventory applications, estimation of mean proportion forest area using Landsat-based independent variables for a study area in the USA and estimation of mean growing stock volume per unit area using lidar-based independent variables for a study area in Norway. Variations of a nonlinear logistic regression model are used for both applications. The results indicate selection of subsets of remotely sensed independent variables to maximize precision had negligible effects on the quality of fit of the models to the data and on estimates of means but substantial proportional beneficial effects on precision. C1 [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Naesset, Erik; Gobakken, Terje] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, N-1432 As, Norway. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us NR 39 TC 7 Z9 7 U1 0 U2 21 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD FEB PY 2013 VL 6 IS 1 SI SI BP 27 EP 34 DI 10.1109/JSTARS.2012.2227299 PG 8 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA 110UG UT WOS:000316471600004 ER PT J AU Pandya, TS Srinivasan, R Johnson, JK AF Pandya, Tejas S. Srinivasan, Radhakrishnan Johnson, Jason K. TI Operating air velocities for fiber separation from corn flour using the Elusieve process SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Elusieve; NDF; Separation; Terminal velocity; Corn; Fiber ID DISTILLERS DRIED GRAINS; SOLUBLES DDGS; CLASSIFICATION AB Fiber separation from corn flour could increase ethanol productivity and increase energy value as feed for non-ruminants (swine and poultry). Elusieve process, a combination of sieving and air classification, has been found to be effective in separating fiber. The objectives of this study were to determine the operating air velocities for corn particles and to compare physical properties of corn particles with that of DDGS particles from an earlier study. The operating air velocities for large, medium and small corn size fractions were 2.9-3.8,2.8-3.0 and 2.5-2.6 m/s, respectively. Densities of nonfiber particles for corn flour were higher than for DDGS (earlier study). Compared to DDGS, the difference between fiber and nonfiber particle terminal velocities was higher for corn, which signifies relative ease of operability for fiber separation from corn flour. (C) 2012 Elsevier B.V. All rights reserved. C1 [Pandya, Tejas S.; Srinivasan, Radhakrishnan] Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Johnson, Jason K.] ARS, USDA, Starkville, MS 39759 USA. RP Srinivasan, R (reprint author), Dept Agr & Biol Engn, 130 Creelman, Mississippi State, MS 39762 USA. EM rs634@msstate.edu FU Sustainable Energy Research Centre (SERC) FX Thanks to Dr. Scott Branton, Dr. Joseph Purswell and William Elliot of USDA ARS for their technical inputs in construction of elutriation column. Thanks to Courtney Paige Thompson and William Fuller for technical assistance. Thanks to Sustainable Energy Research Centre (SERC) for partial funding towards this work. NR 14 TC 1 Z9 1 U1 4 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2013 VL 45 BP 100 EP 105 DI 10.1016/j.indcrop.2012.11.045 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA 115SK UT WOS:000316832000015 ER PT J AU Cruz, VV Walters, CT Dierig, DA AF Cruz, Von Mark V. Walters, Christina T. Dierig, David A. TI Dormancy and after-ripening response of seeds from natural populations and conserved Physaria (syn. Lesquerella) germplasm and their association with environmental and plant parameters SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE After-ripening; Brassicaceae; Bladderpod; Seed dormancy; Seed pretreatment ID DESERT MUSTARD; GERMINATION; FENDLERI; TEMPERATURE; STORAGE; GROWTH; L.; ARABIDOPSIS; EVOLUTION; BREAKING AB Seed dormancy studies in Physaria are still limited to date. To further understand this trait as well as the after-ripening response in these new crop taxa, we sampled different seed lots of genebank conserved accessions and natural populations of Physaria fendleri (syn. Lesquerella fendleri) and Physaria gordonii (syn. L gordonii) in the U.S. Southwest. We subjected seeds from the natural populations to different after-ripening regimens, storing them over two saturated salt solutions (LiCl and MgCl2) to equilibrate seed moisture levels, at three storage temperatures (5, 25, and 35 degrees C) for various lengths of time (4, 8, and 12 weeks) and then germinated the seeds at different temperatures (constant 24 degrees C and alternating 15/25 degrees C), while seeds from the conserved accessions to 4 and 12 weeks storage at MgCl2 and at an alternating 15/25 degrees C. Results obtained from the populations indicate significant differences for total germination among storage durations and between germination treatments. In contrast, no significant difference in total germination was found for seeds of the conserved accessions between storage durations, even with gibberellic acid supplementation. We further explored possible associations of the observed germination responses to climatic data and other parameters recorded from the natural populations. Published by Elsevier B.V. C1 [Cruz, Von Mark V.; Walters, Christina T.; Dierig, David A.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Cruz, Von Mark V.] Colorado State Univ, Dept Bioagr Sci & Pest Mgt, Ft Collins, CO 80523 USA. RP Dierig, DA (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM david.dierig@ars.usda.gov NR 65 TC 1 Z9 1 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2013 VL 45 BP 191 EP 199 DI 10.1016/j.indcrop.2012.12.018 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA 115SK UT WOS:000316832000027 ER PT J AU Hojilla-Evangelista, MP Evangelista, RL Isbell, TA Selling, GW AF Hojilla-Evangelista, Mila P. Evangelista, Roque L. Isbell, Terry A. Selling, Gordon W. TI Effects of cold-pressing and seed cooking on functional properties of protein in pennycress (Thlaspi arvense L.) seed and press cakes SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Pennycress; Pennycress proteins; Oil processing; Protein functionality ID RAPESEED PROTEIN; OIL; SOLUBILITY; ULTRAFILTRATION; EXTRACTION AB Current interest in pennycress (Thlaspi arvense L.) comes from its seed oil, which is being evaluated for biofuel production. The seed also has notable protein content (27% moisture-free, oil-free basis). The effects of oil processing conditions on functionality of pennycress seed proteins were determined to identify potential uses for the meal. Whole seeds were either simply cold-pressed or heated at 82 degrees C with residence time of 50 min in the seed conditioner. Oil was extracted by screw-pressing. Composition and functional properties (solubility, foaming, emulsification, water-holding capacity) of extractable proteins in press cakes and unprocessed pennycress seed were determined and compared. Pennycress seed protein had predominantly albumins and globulins, no prolamins, and few glutelins. Cooking significantly reduced the amounts of albumins and globulins in the press cake. All samples showed the lowest solubility (10%) at pH 4 and only moderate solubility (35-45%) as pH increased. Both seed and press cake proteins had excellent foaming and emulsifying properties, but press cake proteins had higher water-holding capacities. These results showed that heat treatment during oil processing adversely affected the albumins and globulins, as well as solubility behavior of protein in pennycress seed and press cake, but the protein still has other useful functional properties. Published by Elsevier B.V. C1 [Hojilla-Evangelista, Mila P.; Selling, Gordon W.] USDA ARS, Plant Polymer Res Unit, NCAUR, Peoria, IL 61604 USA. [Evangelista, Roque L.; Isbell, Terry A.] USDA ARS, Biooils Res Unit, NCAUR, Peoria, IL 61604 USA. RP Hojilla-Evangelista, MP (reprint author), USDA ARS, Plant Polymer Res Unit, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM Mila.HojillaEvangelista@ars.usda.gov NR 28 TC 14 Z9 15 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2013 VL 45 BP 223 EP 229 DI 10.1016/j.indcrop.2012.12.026 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA 115SK UT WOS:000316832000031 ER PT J AU Gesch, RW AF Gesch, R. W. TI Growth and yield response of calendula (Calendula officinalis) to sowing date in the northern US SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Calendula; Sowing date; Yield; Seed oil ID SEED YIELD; PLANT-POPULATION; CROP; OIL; ASTERACEAE AB Calendula (Calendula officinalis L.) seed is a rich source of the conjugated C18:3 fatty acid calendic acid and can serve as a replacement for volatile organic compounds in many industrial chemicals such as paints, coatings and adhesives. Calendula is widely adapted to temperate climates and may be a beneficial rotational crop for the northern U.S. where crop diversity is lacking, while potentially providing producers with a new economic opportunity. However, very little is known about its agronomic potential for the U.S. or best management practices for its production. Therefore, a two-year study was conducted in west central Minnesota to evaluate the growth and yield response of calendula to sowing date. One open pollinated calendula cultivar, Carola, and two hybrids, 1557 and 99276, were sown at two-week intervals between early-May and mid-June. Final plant population density was greatest for the early-June sowing (139 plants m(-2)) and declined with earlier or later sowing. Based on the combined analysis of both years, mean seed yield ranged from 1166 to 1839 kg ha(-1) and was greatest for the early-May sowing and declined thereafter. Hybrid 99276 gave the greatest seed yield, which was as high as 2380 kg ha(-1) for the early-May sowing in 2009. Seed oil content averaged 19.4% and did not vary with sowing date, although Carola had slightly greater oil content at 20.5% than hybrids 99276 and 1557, which were 19 and 18.6%, respectively. The number of days from planting to 50% flowering ranged from 52 to 59 d and from planting to harvest about 103 to 115 d. Results indicate that calendula flourishes well in the northern Corn Belt and can be planted and harvested as early as most cold tolerant small grains making it a potentially attractive rotational crop for this region. Published by Elsevier B.V. C1 USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Gesch, RW (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM russ.gesch@ars.usda.gov NR 25 TC 3 Z9 3 U1 5 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2013 VL 45 BP 248 EP 252 DI 10.1016/j.indcrop.2012.11.046 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA 115SK UT WOS:000316832000035 ER PT J AU Rashid, U Ibrahim, M Yasin, S Yunus, R Taufiq-Yap, YH Knothe, G AF Rashid, Umer Ibrahim, Muhammad Yasin, Shahid Yunus, Robiah Taufiq-Yap, Y. H. Knothe, Gerhard TI Biodiesel from Citrus reticulata (mandarin orange) seed oil, a potential non-food feedstock SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biodiesel; Citrus reticulata; Fuel properties; Methyl esters ID FATTY-ACID-COMPOSITION; FUEL PROPERTIES; METHYL-ESTERS; COMPONENTS; TRANSESTERIFICATION; SPECTROSCOPY; PETRODIESEL; WASTE AB Oil extracted from Citrus reticulata (mandarin orange) seeds was investigated as a potential feedstock for the production of biodiesel. The biodiesel fuel was prepared by sodium methoxide-catalyzed transesterification of the oil with methanol. Fuel properties that were determined include cetane number, cloud, pour, and cold filter plugging points, kinematic viscosity, oxidative stability, flash point, sulfur content, ash content, density and acid value. The citrus seed oil methyl esters were found to satisfy both ASTM D6751 and EN 14214 biodiesel standards. The NMR spectra of the methyl esters of C. reticulata seed oil are reported. Published by Elsevier B.V. C1 [Rashid, Umer; Yunus, Robiah] Univ Putra Malaysia, Inst Adv Technol, Upm Serdang 43400, Selangor, Malaysia. [Rashid, Umer; Yasin, Shahid] Govt Coll Univ, Dept Ind Chem, Faisalabad 38000, Pakistan. [Ibrahim, Muhammad] Govt Coll Univ, Dept Environm Sci, Faisalabad 38000, Pakistan. [Taufiq-Yap, Y. H.] Univ Putra Malaysia, Ctr Excellence Catalysis Sci & Technol, Fac Sci, Upm Serdang 43400, Selangor, Malaysia. [Knothe, Gerhard] ARS, NCAUR, USDA, Peoria, IL 61604 USA. RP Knothe, G (reprint author), ARS, NCAUR, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM umer.rashid@yahoo.com; gerhard.knothe@ars.usda.gov RI Yunus, Robiah/J-4542-2013; Rashid, Umer/C-9986-2011 OI Yunus, Robiah/0000-0002-3650-1291; Rashid, Umer/0000-0001-6224-413X NR 49 TC 20 Z9 20 U1 2 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2013 VL 45 BP 355 EP 359 DI 10.1016/j.indcrop.2012.12.039 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA 115SK UT WOS:000316832000050 ER PT J AU Bouskill, NJ Lim, HC Borglin, S Salve, R Wood, TE Silver, WL Brodie, EL AF Bouskill, Nicholas J. Lim, Hsiao Chien Borglin, Sharon Salve, Rohit Wood, Tana E. Silver, Whendee L. Brodie, Eoin L. TI Pre-exposure to drought increases the resistance of tropical forest soil bacterial communities to extended drought SO ISME JOURNAL LA English DT Article DE resistance; pyrosequencing; drought effects; temporal ID MICROBIAL COMMUNITIES; ORGANIC-MATTER; CARBON-DIOXIDE; ECOSYSTEM FUNCTION; IRON REDUCTION; ATACAMA DESERT; NITROUS-OXIDE; NITRIC-OXIDE; RAIN-FOREST; CLIMATE AB Global climate models project a decrease in the magnitude of precipitation in tropical regions. Changes in rainfall patterns have important implications for the moisture content and redox status of tropical soils, yet little is known about how these changes may affect microbial community structure. Specifically, does exposure to prior stress confer increased resistance to subsequent perturbation? Here we reduced the quantity of precipitation throughfall to tropical forest soils in the Luquillo Mountains, Puerto Rico. Treatments included newly established throughfall exclusion plots (de novo excluded), plots undergoing reduction for a second time (pre-excluded) and ambient control plots. Ten months of throughfall exclusion led to a small but statistically significant decline in soil water potential and bacterial populations clearly adapted to increased osmotic stress. Although the water potential decline was small and microbial biomass did not change, phylogenetic diversity in the de novo-excluded plots decreased by similar to 40% compared with the control plots, yet pre-excluded plots showed no significant change. On the other hand, the relative abundances of bacterial taxa in both the de novo-excluded and pre-excluded plots changed significantly with throughfall exclusion compared with control plots. Changes in bacterial community structure could be explained by changes in soil pore water chemistry and suggested changes in soil redox. Soluble iron declined in treatment plots and was correlated with decreased soluble phosphorus concentrations, which may have significant implications for microbial productivity in these P-limited systems. The ISME Journal (2013) 7, 384-394; doi:10.1038/ismej.2012.113; published online 15 November 2012 C1 [Bouskill, Nicholas J.; Lim, Hsiao Chien; Borglin, Sharon; Brodie, Eoin L.] Lawrence Berkeley Natl Lab, Dept Ecol, Div Earth Sci, Berkeley, CA 94702 USA. [Salve, Rohit] Lawrence Berkeley Natl Lab, Hydrol Dept, Berkeley, CA 94702 USA. [Wood, Tana E.; Silver, Whendee L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Wood, Tana E.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR USA. RP Bouskill, NJ (reprint author), Lawrence Berkeley Natl Lab, Dept Ecol, Div Earth Sci, 70A-3317, Berkeley, CA 94702 USA. EM njbouskill@lbl.gov; elbrodie@lbl.gov RI Brodie, Eoin/A-7853-2008; Bouskill, Nick/G-2390-2015; Borglin, Sharon/I-1013-2016 OI Brodie, Eoin/0000-0002-8453-8435; FU NSF; DOE; Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory; Office of Science, of the US Department of Energy [DE-AC02-05CH11231] FX We thank Julian Fourtney and Kristin DeAngelis for assistance in the field, Joern Larsen and April Van Hise for ICP-MS analyses. NOAA Climate and Global Change postdoctoral fellowship to TEW. This work was partially supported by an NSF grant and DOE grants to WLS and by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory, by the Director, Office of Science, of the US Department of Energy under Contract No. DE-AC02-05CH11231. NR 67 TC 21 Z9 21 U1 6 U2 89 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 EI 1751-7370 J9 ISME J JI ISME J. PD FEB PY 2013 VL 7 IS 2 BP 384 EP 394 DI 10.1038/ismej.2012.113 PG 11 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 114EI UT WOS:000316723300015 PM 23151641 ER PT J AU Hartney, SL Mazurier, S Girard, MK Mehnaz, S Davis, EW Gross, H Lemanceau, P Loper, JE AF Hartney, Sierra L. Mazurier, Sylvie Girard, Maeva K. Mehnaz, Samina Davis, Edward W., II Gross, Harald Lemanceau, Philippe Loper, Joyce E. TI Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5 SO JOURNAL OF BACTERIOLOGY LA English DT Article ID NONRIBOSOMAL PEPTIDE SYNTHETASES; NUCLEOTIDE-SEQUENCE ANALYSIS; SWISS-MODEL WORKSPACE; FERRIPYOVERDINE RECEPTOR; FLUORESCENT PSEUDOMONAS; PSEUDOBACTIN RECEPTOR; PUTIDA WCS358; NONFLUORESCENT PSEUDOMONAS; HETEROLOGOUS SIDEROPHORES; BACTERIAL SIDEROPHORES AB The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric complexes of some pyoverdines produced by other strains of Pseudomonas spp. as sources of iron. Previously, phylogenetic analysis of the 45 TonB-dependent outer membrane proteins in Pf-5 indicated that six are in a well-supported clade with ferric-pyoverdine receptors (Fpvs) from other Pseudomonas spp. We used a combination of phylogenetics, bioinformatics, mutagenesis, pyoverdine structural determinations, and cross-feeding bioassays to assign specific ferric-pyoverdine substrates to each of the six Fpvs of Pf-5. We identified at least one ferric-pyoverdine that was taken up by each of the six Fpvs of Pf-5. Functional redundancy of the Pf-5 Fpvs was also apparent, with some ferric-pyoverdines taken up by all mutants with a single Fpv deletion but not by a mutant having deletions in two of the Fpv-encoding genes. Finally, we demonstrated that phylogenetically related Fpvs take up ferric complexes of structurally related pyoverdines, thereby establishing structure-function relationships that can be employed in the future to predict the pyoverdine substrates of Fpvs in other Pseudomonas spp. C1 [Hartney, Sierra L.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Mazurier, Sylvie; Lemanceau, Philippe] INRA, UMR Agroecol 1347, F-21034 Dijon, France. [Girard, Maeva K.; Gross, Harald] Univ Bonn, Inst Pharmaceut Biol, Bonn, Germany. [Mehnaz, Samina] Forman Christian Coll Univ, Dept Biol Sci, Lahore, Pakistan. [Davis, Edward W., II; Loper, Joyce E.] Agr Res Serv, USDA, Hort Crops Res Lab, Corvallis, OR USA. RP Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM Joyce.Loper@ars.usda.gov RI Mehnaz, Samina/I-1533-2015; OI Loper, Joyce/0000-0003-3501-5969 FU USDA National Institute of Food and Agriculture [2006-35319-17427, 2008-35600-18770]; Alexander von Humboldt Foundation (Georg Forster Fellowship) FX H. Gross and S. Mehnaz gratefully acknowledge the generous contribution of the Alexander von Humboldt Foundation, which provided financial support (Georg Forster Fellowship awarded to S. Mehnaz). This work was supported by grants 2006-35319-17427 and 2008-35600-18770 from the USDA National Institute of Food and Agriculture. NR 80 TC 13 Z9 13 U1 0 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD FEB PY 2013 VL 195 IS 4 BP 765 EP 776 DI 10.1128/JB.01639-12 PG 12 WC Microbiology SC Microbiology GA 117NW UT WOS:000316961200014 PM 23222724 ER PT J AU Adams, C Frantz, J Bugbee, B AF Adams, Curtis Frantz, Jonathan Bugbee, Bruce TI Macro- and micronutrient-release characteristics of three polymer-coated fertilizers: Theory and measurements SO JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE LA English DT Article DE controlled release; Nutricote; Osmocote; Polyon; slow release ID CONTROLLED NUTRIENT RELEASE; DIFFUSION RELEASE; POTASSIUM RELEASE; AMMONIUM-NITROGEN; NITRATE-NITROGEN; PHOSPHORUS; PATTERNS; MODEL; TEMPERATURE; POPULATION AB In spite of several published studies we have an incomplete understanding of the ion-release mechanisms and characteristics of polymer-coated fertilizers (PCF). Here we extend current conceptual models describing release mechanisms and describe the critical effects of substrate moisture and temperature on macro-and micronutrient release of three PCF types: Polyon (R), Nutricote (R), and Osmocote (R). Nutrient release was quantified at weekly intervals for up to 300 d from 5 degrees C to 40 degrees C in water and chemically inert sand, substrates that allowed release quantification without confounding effects of ion sorption/desorption. At least two release-timeframe formulations of each PCF type were studied and all products had similar nutrient concentrations to allow isolation of the effect of coating technology. Contrary to several studies, our data and model indicate that there is no significant difference in nutrient-release rates in water and a moist, solid substrate. This means that release rates determined in water can be used to model bio-available nutrient concentrations in moist soil or soilless media where sorption/desorption properties alter concentrations after release. Across all PCF, the nutrients most affected by temperature were typically N, K, B, Cu, and Zn, while the least affected were P, Mg, and Fe. We also found consistent differences among the coating technologies. Osmocote fertilizers released faster than specified at both high and low temperatures. Nutricote had relatively steady release rates over time and a nonlinear response to temperature. Polyon released more slowly than specified but replicate samples were highly uniform. C1 [Adams, Curtis; Bugbee, Bruce] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. [Frantz, Jonathan] USDA ARS, Greenhouse Prod Res Grp, Toledo, OH 43606 USA. RP Adams, C (reprint author), Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. EM curtis.b.adams@gmail.com FU NASA; USDA-ARS; Utah Agricultural Experiment Station, Utah State University FX This research was funded by a grant from NASA and supported by the USDA-ARS. We thank Douglas Sturtz and Russell Friedrich for assistance with nutrient analysis. This research was also supported by the Utah Agricultural Experiment Station, Utah State University, and approved as journal paper number 8405. NR 27 TC 12 Z9 14 U1 8 U2 83 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 1436-8730 J9 J PLANT NUTR SOIL SC JI J. Plant Nutr. Soil Sci. PD FEB PY 2013 VL 176 IS 1 BP 76 EP 88 DI 10.1002/jpln.201200156 PG 13 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA 116XH UT WOS:000316916100011 ER PT J AU Zheng, H Wang, ZY Deng, X Zhao, J Luo, Y Novak, J Herbert, S Xing, BS AF Zheng, Hao Wang, Zhenyu Deng, Xia Zhao, Jian Luo, Ye Novak, Jeff Herbert, Stephen Xing, Baoshan TI Characteristics and nutrient values of biochars produced from giant reed at different temperatures SO BIORESOURCE TECHNOLOGY LA English DT Article DE Biochar; Pyrolysis temperature; Giant reed; Nutrient values; Adsorption ID BLACK CARBON; PYROLYSIS; SORPTION; MANURE; SOILS; CONTAMINANTS; REMEDIATION; NITROGEN; REMOVAL; PINE AB To investigate the effect of pyrolysis temperature on properties and nutrient values, biochars were produced from giant reed (Arundo donax L.) at 300-600 degrees C and their properties such as elemental and mineral compositions, release of N, P and K, and adsorption of N and P were determined. With increasing temperatures, more N was lost and residual N was transformed into heterocyclic-N, whereas no P and K losses were observed. P was transformed to less soluble minerals, resulting in a reduction in available-P in high-temperature biochars. A pH of <= 5 favored release of NH4+, PO43- and K+ into water. Low-temperature biochars <= 400 degrees C) showed appreciable NH4+ adsorption (2102 mg kg(-1)). These results indicate that low-temperatures may be optimal for producing biochar from giant reed to improve the nutrient availability. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Zheng, Hao; Wang, Zhenyu; Deng, Xia; Luo, Ye] Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China. [Zheng, Hao; Zhao, Jian; Herbert, Stephen; Xing, Baoshan] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. [Novak, Jeff] ARS, USDA, Coastal Plain Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Wang, ZY (reprint author), Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China. EM wang0628@ouc.edu.cn; bx@umass.edu RI Zheng, Hao/E-4963-2013; Zhao, Jian/D-2798-2012; Zheng, Hao/R-7048-2016 OI Zheng, Hao/0000-0003-4311-5185 FU National Natural Science Foundation of China [41120134004]; USDA Hatch Program [MAS 00982] FX This study was supported by the National Natural Science Foundation of China (41120134004) and USDA Hatch Program (MAS 00982). We are greatly grateful to Dr. Sarah Weis and Dr. Di Zhang for their help with the experiments collecting the data of NH4+, NO3- and total K. NR 35 TC 40 Z9 52 U1 10 U2 127 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2013 VL 130 BP 463 EP 471 DI 10.1016/j.biortech.2012.12.044 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 104YI UT WOS:000316032300062 PM 23313694 ER PT J AU Avci, A Saha, BC Dien, BS Kennedy, GJ Cotta, MA AF Avci, Ayse Saha, Badal C. Dien, Bruce S. Kennedy, Gregory J. Cotta, Michael A. TI Response surface optimization of corn stover pretreatment using dilute phosphoric acid for enzymatic hydrolysis and ethanol production SO BIORESOURCE TECHNOLOGY LA English DT Article DE Corn stover; Dilute phosphoric acid pretreatment; Response surface methodology; Recombinant Escherichia coli FBR5; Ethanol fermentation ID COLI STRAIN FBR5; WHEAT-STRAW; BIOFUELS PRODUCTION; FERMENTATION; SACCHARIFICATION; LAND; FOOD AB Dilute H3PO4 (0.0-2.0%, v/v) was used to pretreat corn stover (10%, w/w) for conversion to ethanol. Pretreatment conditions were optimized for temperature, acid loading, and time using central composite design. Optimal pretreatment conditions were chosen to promote sugar yields following enzymatic digestion while minimizing formation of furans, which are potent inhibitors of fermentation. The maximum glucose yield (85%) was obtained after enzymatic hydrolysis of corn stover pretreated with 0.5% (v/v) acid at 180 degrees C for 15 min while highest yield for xylose (91.4%) was observed from corn stover pretreated with 1% (v/v) acid at 160 degrees C for 10 min. About 26.4 +/- 0.1 g ethanol was produced per L by recombinant Escherichia coli strain FBR5 from 55.1 +/- 1.0 g sugars generated from enzymatically hydrolyzed corn stover (10%, w/w) pretreated under a balanced optimized condition (161.81 degrees C, 0.78% acid, 9.78 min) where only 0.4 +/- 0.0 g furfural and 0.1 +/- 0.0 hydroxylmethyl furfural were produced. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Avci, Ayse; Saha, Badal C.; Dien, Bruce S.; Kennedy, Gregory J.; Cotta, Michael A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Avci, Ayse] Sakarya Univ, Dept Food Engn, Fac Engn, TR-54187 Sakarya, Turkey. RP Avci, A (reprint author), Sakarya Univ, Dept Food Engn, Fac Engn, TR-54187 Sakarya, Turkey. EM aysea@sakarya.edu.tr OI Cotta, Michael/0000-0003-4565-7754; Dien, Bruce/0000-0003-3863-6664 FU Turkish Council of Higher Education FX Ayse Avci gratefully acknowledges the fellowship offered by the Turkish Council of Higher Education to conduct this research and Sakarya University for study leave. NR 34 TC 35 Z9 38 U1 2 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2013 VL 130 BP 603 EP 612 DI 10.1016/j.biortech.2012.12.104 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 104YI UT WOS:000316032300081 PM 23334017 ER PT J AU Wang, ZJ Qin, MH Zhu, JY Tian, GY Li, ZQ AF Wang, Zhaojiang Qin, Menghua Zhu, J. Y. Tian, Guoyu Li, Zongquan TI Evaluation energy efficiency of bioconversion knot rejects to ethanol in comparison to other thermochemically pretreated biomass SO BIORESOURCE TECHNOLOGY LA English DT Article DE Bioconversion; Energy efficiency; Ethanol; Knot rejects; Physical refining ID FUEL ETHANOL; LIGNOCELLULOSIC BIOMASS; WASTE; TECHNOLOGIES; HYDROLYSIS; PRODUCTS; PULP AB Rejects from sulfite pulp mill that otherwise would be disposed of by incineration were converted to ethanol by a combined physical biological process that was comprised of physical refining and simultaneous saccharification and fermentation (SSF). The energy efficiency was evaluated with comparison to thermochemically pretreated biomass, such as those pretreated by dilute acid (DA) and sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL). It was observed that the structure deconstruction of rejects by physical refining was indispensable to effective bioconversion but more energy intensive than that of thermochemically pretreated biomass. Fortunately, the energy consumption was compensated by the reduced enzyme dosage and the elevated ethanol yield. Furthermore, adjustment of disk-plates gap led to reduction in energy consumption with negligible influence on ethanol yield. In this context, energy efficiency up to 717.7% was achieved for rejects, much higher than that of SPORL sample (283.7%) and DA sample (152.8%). (C) 2012 Elsevier Ltd. All rights reserved. C1 [Wang, Zhaojiang; Qin, Menghua; Tian, Guoyu; Li, Zongquan] Shandong Polytech Univ, Key Lab Paper Sci & Technol, Minist Educ, Jinan 250353, Peoples R China. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. RP Wang, ZJ (reprint author), Shandong Polytech Univ, Univ Pk Sci & Technol, Jinan 250353, Peoples R China. EM wzj820415@gmail.com; jzhu@fs.fed.us FU US Forest Service (USFS) through the Program of Woody Biomass, Bioenergy, and Bioproducts (WBBB); Chinese Scholarship Council (CSC) FX This work was conducted at the USDA Forest Service, Forest Products Laboratory while Wang was a visiting scientist and on official U.S. government time of Zhu. This work was sponsored by the US Forest Service (USFS) through the Program of Woody Biomass, Bioenergy, and Bioproducts (WBBB, 2011). We acknowledge Diane Dietrich of US Forest Service, Forest Products Laboratory (USFS-FPL) for carrying out many careful analyses of ethanol in fermentation broth samples, and Fred Matt (USFS-FPL) for carbohydrate composition analysis of the solid substrates. The Chinese Scholarship Council (CSC) partially provided financial support to Wang for his visiting appointment at USFS-FPL. NR 27 TC 7 Z9 7 U1 1 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2013 VL 130 BP 783 EP 788 DI 10.1016/j.biortech.2012.12.058 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 104YI UT WOS:000316032300105 PM 23376154 ER PT J AU Horton, DR AF Horton, David R. TI Previous exposure to other males leads to prolonged copulation by a predatory true bug (Hemiptera: Heteroptera: Anthocoridae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID OVARIAN DEVELOPMENT; SEX-RATIO; DURATION; INSEMINATION; POPULATIONS; LYGAEIDAE; DENSITY AB Mating pairs of Anthocoris whitei Reuter (Hemiptera: Heteroptera: Anthocoridae) irregularly exhibit copulations exceeding five hours in duration. In other Heteroptera, male-biased sex ratios or high densities of male conspecifics may lead to prolonged copulation. I tested whether exposure to conspecific males preceding access to females led to prolonged copulations by A. whitei. Copulations in which the male had been exposed to other males were significantly longer (by 60+ minutes) than copulations in which the male had not been exposed to other males. Durations exceeded five hours in several pairings following exposure to other males. Almost 75% of copulations in which males had not been exposed to other males were <100 minutes in duration, whereas only 22%-29% of copulations involving males that had been exposed to other males were <100 minutes in duration. Atypically long copulations by this species may be a form of postinsemination mate guarding to prevent insemination of a guarded female by other males. C1 ARS, USDA, Wapato, WA 98951 USA. RP Horton, DR (reprint author), ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM David.Horton@ars.usda.gov NR 22 TC 0 Z9 0 U1 1 U2 6 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD FEB PY 2013 VL 145 IS 1 BP 88 EP 93 DI 10.4039/tce.2012.91 PG 6 WC Entomology SC Entomology GA 112RD UT WOS:000316609400011 ER PT J AU Baker, BW Augustine, DJ Sedgwick, JA Lubow, BC AF Baker, Bruce W. Augustine, David J. Sedgwick, James A. Lubow, Bruce C. TI Ecosystem engineering varies spatially: a test of the vegetation modification paradigm for prairie dogs SO ECOGRAPHY LA English DT Article ID BLACK-TAILED PRAIRIE; MIXED-GRASS PRAIRIE; DESERT GRASSLAND; RODENT COMMUNITIES; CYNOMYS-GUNNISONI; SHORTGRASS STEPPE; MOUNTAIN PLOVERS; GREAT-PLAINS; COLONIES; HETEROGENEITY AB Colonial, burrowing herbivores can be engineers of grassland and shrubland ecosystems worldwide. Spatial variation in landscapes suggests caution when extrapolating single-place studies of single species, but lack of data and the need to generalize often leads to model system' thinking and application of results beyond appropriate statistical inference. Generalizations about the engineering effects of prairie dogs (Cynomys sp.) developed largely from intensive study at a single complex of black-tailed prairie dogs C. ludovicianus in northern mixed prairie, but have been extrapolated to other ecoregions and prairie dog species in North America, and other colonial, burrowing herbivores. We tested the paradigm that prairie dogs decrease vegetation volume and the cover of grasses and tall shrubs, and increase bare ground and forb cover. We sampled vegetation on and off 279 colonies at 13 complexes of 3 prairie dog species widely distributed across 5 ecoregions in North America. The paradigm was generally supported at 7 black-tailed prairie dog complexes in northern mixed prairie, where vegetation volume, grass cover, and tall shrub cover were lower, and bare ground and forb cover were higher, on colonies than at paired off-colony sites. Outside the northern mixed prairie, all 3 prairie dog species consistently reduced vegetation volume, but their effects on cover of plant functional groups varied with prairie dog species and the grazing tolerance of dominant perennial grasses. White-tailed prairie dogs C. leucurus in sagebrush steppe did not reduce shrub cover, whereas black-tailed prairie dogs suppressed shrub cover at all complexes with tall shrubs in the surrounding habitat matrix. Black-tailed prairie dogs in shortgrass steppe and Gunnison's prairie dogs C. gunnisoni in Colorado Plateau grassland both had relatively minor effects on grass cover, which may reflect the dominance of grazing-tolerant shortgrasses at both complexes. Variation in modification of vegetation structure may be understood in terms of the responses of different dominant perennial grasses to intense defoliation and differences in foraging behavior among prairie dog species. Spatial variation in the engineering role of prairie dogs suggests spatial variation in their keystone role, and spatial variation in the roles of other ecosystem engineers. Thus, ecosystem engineering can have a spatial component not evident from single-place studies. C1 [Baker, Bruce W.; Sedgwick, James A.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Augustine, David J.] ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Lubow, Bruce C.] Colorado State Univ, Ft Collins, CO 80523 USA. RP Baker, BW (reprint author), US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. EM david.augustine@ars.usda.gov FU U.S. Geological Survey, Fort Collins, Colorado, USA FX We thank all the dedicated field research assistants who contributed to the study: M. Bailey, R. Browning, C. Cook, K. Johnston, J. Kelly, H. McCann, C. Mui, A. Quinn, T. Toombs, T. Beck, A. Boyle, J. Brooks, L. Comita, H. Ducharme, K. Kump, L. Locke, R. Scott, C. Smith, and M. Ward. Phil Chapman of Colorado State Univ. provided helpful statistical advice. We also thank the many biologists, land managers, tribal leaders, private landowners, and others who provided field support. We thank P. Stapp and J. Derner for helpful comments on a previous version of the paper. Field data collection was funded by the U.S. Geological Survey, Fort Collins, Colorado, USA. NR 51 TC 5 Z9 5 U1 10 U2 88 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-7590 J9 ECOGRAPHY JI Ecography PD FEB PY 2013 VL 36 IS 2 BP 230 EP 239 DI 10.1111/j.1600-0587.2012.07614.x PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 108XR UT WOS:000316330100013 ER PT J AU Sampson, BJ Stringer, SJ Marshall, DA AF Sampson, Blair J. Stringer, Stephen J. Marshall, Donna A. TI Blueberry Floral Attributes and Their Effect on the Pollination Efficiency of an Oligolectic Bee, Osmia ribifloris Cockerell (Megachilidae: Apoidea) SO HORTSCIENCE LA English DT Article DE flower; morphology; fruit set; seed number; pollinator; Vaccinium; virgatum; ashei; corymbosum; darrowii; elliottii; myrsinites; tenellum ID SOUTHERN HIGHBUSH BLUEBERRY; RABBITEYE BLUEBERRY; VACCINIUM-CORYMBOSUM; SHARPBLUE BLUEBERRY; FLOWER SIZE; HYMENOPTERA; SHAPE AB We evaluated relationships between floral traits of 23 genotypes of southern blueberries and indices of pollination efficiency (fruit set, fruit abortion, seed number, and berry size) for Osmia ribifloris Cockerell, a manageable solitary bee. Flower size in Vaccinium and presumably ovary size were proportional to berry size, except for the tiny blooms of one V. tenellum clone (NC7808), which produce large commercial-sized berries of approximate to 2 g. Longer-styled blueberry flowers visited by O. ribifloris produced the heaviest berries with the most seeds. Osmia ribifloris reliably pollinated 'Climax' and 'Tifblue' rabbiteye blueberries. However, the peculiarly misshapen blooms of 'Premier' rabbiteye blueberry receive less pollination from O. ribifloris and yield berries containing 25% fewer seeds. Fruit set for these misshapen 'Premier' flowers was equivalent to that of intact flowers indicating that this floral polymorphism would not greatly alter cultivar performance. For seven Vaccinium species, wild and cultivated alike, 80% to 100% of a plant's fruit production depends on efficient cross-pollination by bees such as O. ribifloris. C1 [Sampson, Blair J.; Stringer, Stephen J.; Marshall, Donna A.] USDA ARS, Thad Cochran Southern Hort Res Lab, Poplarville, MS 39470 USA. RP Sampson, BJ (reprint author), USDA ARS, Thad Cochran Southern Hort Res Lab, POB 287,810 Highway 26 West, Poplarville, MS 39470 USA. EM blair.sampson@ars.usda.gov NR 36 TC 3 Z9 3 U1 4 U2 30 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD FEB PY 2013 VL 48 IS 2 BP 136 EP 142 PG 7 WC Horticulture SC Agriculture GA 102GT UT WOS:000315833600001 ER PT J AU Bell, RL AF Bell, Richard L. TI Host Resistance to Pear Psylla of Breeding Program Selections and Cultivars SO HORTSCIENCE LA English DT Article DE Pyrus; antixenosis; Cacopsylla pyricola Forster; Hemiptera; Psyllidae; nymphal feeding; genetic resources ID COLLECTING PYRUS GERMPLASM; SUSCEPTIBLE PEAR; HOMOPTERA; BEHAVIOR; NYMPHS AB Twenty-one pear cultivars and breeders' selections with interspecific pedigrees involving Pyrus ussuriensis Max. or P. pyrifolia (Burm.) Nakai crossed with P. communis were assessed for resistance to pear psylla (Cacopsylla pyricola Forster) using a nymphal feeding antixenosis assay. The proportion of nymphs live and present on the plants varied from 0.52 for Purdue 77-73, a P. ussuriensis X P. communis hybrid, to 0.08 for 'Zelinka', a P. communis cultivar from eastern Europe. Two P. ussuriensis X P. communis hybrid selections (NY 10355 and NY 10359) and the P. communis landrace cultivars, Batjarka and Zelinka, were the most resistant to nymphal feeding. NJ B9 T1 T117 may also be a useful source of resistance. The most resistant germplasm should be valuable genetic resources for the breeding of new pear cultivars with resistance to pear psylla. C1 USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Bell, RL (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM richard.bell@ars.usda.gov NR 26 TC 2 Z9 2 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD FEB PY 2013 VL 48 IS 2 BP 143 EP 145 PG 3 WC Horticulture SC Agriculture GA 102GT UT WOS:000315833600002 ER PT J AU Donahoo, RS Turechek, WW Thies, JA Kousik, CS AF Donahoo, Ryan S. Turechek, William W. Thies, Judy A. Kousik, Chandrasekar S. TI Potential Sources of Resistance in US Cucumis melo PIs to Crown Rot Caused by Phytophthora capsici SO HORTSCIENCE LA English DT Article DE cucurbit; melon; host plant resistance; oomycete; Phytophthora capsici ID PLANT INTRODUCTIONS; VEGETABLE CROPS; GERM PLASM; FRUIT ROT; WATERMELON; PUMPKIN; PEPPER; ROOT; IDENTIFICATION; INSENSITIVITY AB Phytophthora capsici is an aggressive pathogen that is distributed worldwide with a broad host range infecting solanaceous, fabaceous, and cucurbitaceous crops. Over the past two decades, increased incidence of Phytophthora blight, particularly in eastern states, has threatened production of many vegetable crops. Cucumis melo L. (honeydew and muskmelon), although especially susceptible to fruit rot, is also highly susceptible to crown rot. Currently, little is known about host resistance to P. capsici in C. melo. To assess crown rot resistance in C. melo seedlings, 308 U.S. PIs, and two commercial cultivars (Athena and Dinero) were grown under greenhouse conditions. Seedlings with three to four true leaves were inoculated with a five-isolate zoospore suspension (1 x 104 zoospores per seedling) at the crown and monitored for 6 weeks. All the susceptible control plants of Athena died within 7 days post-inoculation. The majority of the PIs (281 of 308) were highly susceptible to crown rot and succumbed to the disease rapidly and had less than 20% of the plants survive. Several PIs (PI 181748, PI 182964, and PI 273438) succumbed to crown rot earlier than the susceptible melon cultivars. Eighty-seven PIs selected on the basis of the first screen were re-evaluated and of these PIs, 44 were less susceptible than cultivars Athena and Dinero. Twenty-five of the 87 PIs were evaluated again and of these six PI, greater than 80% of the plants survived in the two evaluations. Disease development was significantly slower on these PIs compared with the susceptible checks. High levels of resistance in S-1 plants of PI 420180, PI 176936, and PI 176940 were observed, which suggests that development of resistant germplasm for use in breeding programs can be accomplished. Further screening and careful selection within each of these PIs can provide a framework for the development of resistant germplasm for use in breeding programs. C1 [Donahoo, Ryan S.; Thies, Judy A.; Kousik, Chandrasekar S.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Turechek, William W.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Kousik, CS (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM shaker.kousik@ars.usda.gov NR 47 TC 1 Z9 1 U1 0 U2 18 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD FEB PY 2013 VL 48 IS 2 BP 164 EP 170 PG 7 WC Horticulture SC Agriculture GA 102GT UT WOS:000315833600006 ER PT J AU Pounders, CT AF Pounders, Cecil T. TI 'Lufkin Red' and 'Lufkin White' Winter-hardy Hibiscus (Hibiscus x laevis All.) SO HORTSCIENCE LA English DT Editorial Material DE leaf spot resistance; Malvaceae; perennials; ornamentals ID MALVACEAE; L. C1 USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. RP Pounders, CT (reprint author), USDA ARS, Thad Cochran Southern Hort Lab, POB 287,810 Highway 26 West, Poplarville, MS 39470 USA. EM Cecil.Pounders@ars.usda.gov NR 10 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD FEB PY 2013 VL 48 IS 2 BP 237 EP 238 PG 2 WC Horticulture SC Agriculture GA 102GT UT WOS:000315833600018 ER PT J AU Clausen, CA Yang, VW AF Clausen, Carol A. Yang, Vina W. TI Colorimetric micro-assay for accelerated screening of mould inhibitors SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE XTT tetrazolium salt; Mould inhibitors; Micro-assay; Mould fungi; Spore germination ID SUSCEPTIBILITY; ASSAY AB Since current standard laboratory methods are time-consuming macro-assays that rely on subjective visual ratings of mould growth, rapid and quantitative laboratory methods are needed to screen potential mould inhibitors for use in and on cellulose-based products. A colorimetric micro-assay has been developed that uses XTT tetrazolium salt to enzymatically assess metabolic activity in mould spores, saving significant time and resources (i.e. wood specimens). Minimal inhibitory concentration (MIC90) of isothiazolinone, a known mould inhibitor, in the XTT assay was the same or within a two-fold dilution of the MIC90 for two methods currently used to determine mould resistance of cellulose-based products. An ATP assay corroborated XTT assay findings; isothiazolinone appeared to be fungicidal rather than fungistatic to the spores of fungi used in this study. After 24 h exposure to the chemical, spores remained inactive indefinitely. The XTT assay would be a useful tool for screening mould inhibitors for numerous applications in addition to those of the forest products industry. Published by Elsevier Ltd. C1 [Clausen, Carol A.; Yang, Vina W.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Clausen, CA (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM clausen@wisc.edu; vyang@fs.fed.us NR 11 TC 2 Z9 2 U1 1 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD FEB PY 2013 VL 77 BP 68 EP 71 DI 10.1016/j.ibiod.2012.11.005 PG 4 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA 110EZ UT WOS:000316426700011 ER PT J AU Charles, T Grimm, CC Landry, S Maleki, SJ AF Charles, Tysheena Grimm, Casey C. Landry, Samuel Maleki, Soheila J. TI Chemical and Structural Alterations to Ara h 2 Following Simulated Roasting SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Charles, Tysheena] Tulane Univ, New Orleans, LA 70118 USA. [Charles, Tysheena; Grimm, Casey C.; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. [Landry, Samuel] Tulane Univ, Hlth Sci Ctr, New Orleans, LA 70118 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB20 EP AB20 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800072 ER PT J AU Chung, SY Reed, S AF Chung, Si-Yin Reed, Shawndrika TI Effect of D-Amino Acids On IgE Binding to Peanut Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Chung, Si-Yin; Reed, Shawndrika] ARS, USDA, SRRC, New Orleans, LA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB20 EP AB20 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800073 ER PT J AU Hunley, B Kewalramani, A Cheng, HP Teuber, SS Maleki, SJ AF Hunley, Brandi Kewalramani, Anupama Cheng, Hsiaopo Teuber, Suzanne S. Maleki, Soheila J. TI Cross-Reactivity Among Almond, Peanut and Other Tree Nuts in Almond and Peanut Allergic Patients SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Hunley, Brandi] Tulane Univ, New Orleans, LA 70118 USA. [Kewalramani, Anupama] Univ Maryland, Sch Med, Laurel, MD USA. [Cheng, Hsiaopo; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. [Teuber, Suzanne S.] Univ Calif Davis, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB20 EP AB20 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800075 ER PT J AU Hurlburt, BK Celeste, L Majorek, KA McBride, J Maleki, SJ Minor, W Chruszcz, M AF Hurlburt, Barry K. Celeste, Lesa Majorek, Karolina A. McBride, Jane Maleki, Soheila J. Minor, Wladek Chruszcz, Maksymilian TI Structure and Function of the Peanut Panallergen Ara h 8 SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Hurlburt, Barry K.; McBride, Jane; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. [Celeste, Lesa; Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. [Majorek, Karolina A.; Minor, Wladek] Univ Virginia, Charlottesville, VA USA. RI Minor, Wladek/F-3096-2014 NR 0 TC 0 Z9 0 U1 0 U2 4 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB19 EP AB19 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800071 ER PT J AU Maleki, SJ Charles, T Grimm, CC Hurlburt, BK Chruszcz, M Cheng, HP Schein, C AF Maleki, Soheila J. Charles, Tysheena Grimm, Casey C. Hurlburt, Barry K. Chruszcz, Maksymilian Cheng, Hsiaopo Schein, Catherine TI Repeated Sequences with Similar Physicochemical Properties May Account for Cross-Reactions Between Peanuts and Tree Nuts SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Maleki, Soheila J.; Charles, Tysheena; Grimm, Casey C.; Hurlburt, Barry K.; Cheng, Hsiaopo] ARS, USDA, SRRC, New Orleans, LA USA. [Charles, Tysheena] Tulane Univ, New Orleans, LA 70118 USA. [Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. [Schein, Catherine] Univ Texas Med Branch, Galveston, TX 77555 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB20 EP AB20 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800074 ER PT J AU Mattison, CP Desormeaux, WA Grimm, CC Wasserman, RL AF Mattison, Christopher P. Desormeaux, Wendy A. Grimm, Casey C. Wasserman, Richard L. TI Characterization of the Effects of Proteolysis and Reduction On Cashew Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Mattison, Christopher P.; Desormeaux, Wendy A.; Grimm, Casey C.] USDA ARS SRRC, New Orleans, LA USA. [Wasserman, Richard L.] Allergy Immunol Res Ctr North Texas, Dallas, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB21 EP AB21 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800077 ER PT J AU Schein, C Teuber, SS Cheng, H Grimm, CC Maleki, SJ AF Schein, Catherine Teuber, Suzanne S. Cheng, Hsiaopo Grimm, Casey C. Maleki, Soheila J. TI Antibodies to the Physiochemical-Consensus Sequence of Jug r 2 Containing Glutamine-Rich Repeats Bind Allergens in Peanuts and Other Tree Nuts SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Schein, Catherine] Univ Texas Med Branch, Galveston, TX 77555 USA. [Teuber, Suzanne S.] Univ Calif Davis, Davis, CA USA. [Cheng, Hsiaopo; Grimm, Casey C.; Maleki, Soheila J.] USDA ARS SRRC, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB21 EP AB21 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800076 ER PT J AU Zhang, YZ Jin, TC Wang, Y AF Zhang, Yuzhu Jin, Tengchuan Wang, Yang TI Crystal Structure of Korean Pine (Pinus koraiensis) Vicilin SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American Academy of Allergy, Asthma and Immunology (AAAAI) CY FEB 22-26, 2013 CL San Antonio, TX SP Amer Acad Allergy, Asthma & Immunol (AAAAI) C1 [Zhang, Yuzhu] USDA, Albany, CA USA. [Zhang, Yuzhu; Jin, Tengchuan; Wang, Yang] IIT, Chicago, IL 60616 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2013 VL 131 IS 2 SU S BP AB17 EP AB17 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 111WC UT WOS:000316550800063 ER PT J AU Weston, LA Alsaadawi, IS Baerson, SR AF Weston, Leslie A. Alsaadawi, Ibrahim S. Baerson, Scott R. TI Sorghum Allelopathy-From Ecosystem to Molecule SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Weed suppression; Allelochemicals; Sorgoleone; Benzoquinone; Phenolics; Root hairs; Biosynthesis; Herbicide ID III POLYKETIDE SYNTHASES; BICOLOR ROOT HAIRS; WEED-CONTROL; ALLELOCHEMICAL SORGOLEONE; BIOSYNTHESIS; RESIDUES; PLANT; WHEAT; SOIL; CROP AB Sorghum allelopathy has been reported in a series of field experiments following sorghum establishment. In recent years, sorghum phytotoxicity and allelopathic interference also have been well-described in greenhouse and laboratory settings. Observations of allelopathy have occurred in diverse locations and with various sorghum plant parts. Phytotoxicity has been reported when sorghum was incorporated into the soil as a green manure, when residues remained on the soil surface in reduced tillage settings, or when sorghum was cultivated as a crop in managed fields. Allelochemicals present in sorghum tissues have varied with plant part, age, and cultivar evaluated. A diverse group of sorghum allelochemicals, including numerous phenolics, a cyanogenic glycoside (dhurrin), and a hydrophobic p-benzoquinone (sorgoleone) have been isolated and identified in recent years from sorghum shoots, roots, and root exudates, as our capacity to analyze and identify complex secondary products in trace quantities in the plant and in the soil rhizosphere has improved. These allelochemicals, particularly sorgoleone, have been widely investigated in terms of their mode(s) of action, specific activity and selectivity, release into the rhizosphere, and uptake and translocation into sensitive indicator species. Both genetics and environment have been shown to influence sorgoleone production and expression of genes involved in sorgoleone biosynthesis. In the soil rhizosphere, sorgoleone is released continuously by living root hairs where it accumulates in significant concentrations around its roots. Further experimentation designed to study the regulation of sorgoleone production by living sorghum root hairs may result in increased capacity to utilize sorghum cover crops more effectively for suppression of germinating weed seedlings, in a manner similar to that of soil-applied preemergent herbicides like trifluralin. C1 [Weston, Leslie A.] Charles Sturt Univ, EH Graham Ctr, Wagga Wagga, NSW 2678, Australia. [Alsaadawi, Ibrahim S.] Univ Baghdad, Coll Sci, Dept Biol, Baghdad, Iraq. [Baerson, Scott R.] ARS, USDA, Nat Prod Utilizat Res Unit, Nat Prod Ctr, Oxford, MS 38655 USA. RP Weston, LA (reprint author), Charles Sturt Univ, EH Graham Ctr, Wagga Wagga, NSW 2678, Australia. EM leweston@csu.edu.au; ibrahimalsadawi@yahoo.com; scott.baerson@ars.usda.gov RI Weston, Leslie/M-4557-2015 OI Weston, Leslie/0000-0002-1029-7982 FU NSW Office of Science and Medical Research FX Professor L. A. Weston acknowledges the support of the NSW Office of Science and Medical Research which awarded her a Biofirst Life Sciences Fellowship in 2008. The authors are appreciative of the manuscript reviews received and acknowledge this assistance in manuscript preparation. NR 84 TC 19 Z9 22 U1 6 U2 138 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2013 VL 39 IS 2 SI SI BP 142 EP 153 DI 10.1007/s10886-013-0245-8 PG 12 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 106BH UT WOS:000316115900002 PM 23393005 ER PT J AU Gealy, D Moldenhauer, K Duke, S AF Gealy, David Moldenhauer, Karen Duke, Sara TI Root Distribution and Potential Interactions Between Allelopathic Rice, Sprangletop (Leptochloa spp.), and Barnyardgrass (Echinochloa crus-galli) based on C-13 Isotope Discrimination Analysis SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE C-13/C-12 Isotope ratio; delta C-13; C-13 depletion; C-3 photosynthetic pathway; C-4 photosynthetic pathway; Crop-weed interference; Crop-weed root distribution; Allelopathic rice; Indica rice ID WATER-USE EFFICIENCY; PADDY FIELD WEEDS; ORYZA-SATIVA; SEEDED RICE; RELATIVE IMPORTANCE; SHOOT COMPETITION; MOMILACTONE-B; UPLAND RICE; CARBON; SUPPRESSION AB Weed-suppressive rice cultivars hold promise for improved and more economical weed management in rice. Interactions between roots of rice and weeds are thought to be modulated by the weed-suppressive activity of some rice cultivars, but these phenomena are difficult to measure and not well understood. Thus, above-ground productivity, weed suppression, and root distribution of 11 rice cultivars and two weed species were evaluated in a drill-seeded, flood-irrigated system at Stuttgart, Arkansas, USA in a two-year study. The allelopathic cultivars, PI 312777 and Taichung Native 1 (TN-1), three other weed-suppressive cultivars, three indica-derived breeding selections, and three non-suppressive commercial cultivars were evaluated in field plots infested with barnyardgrass (Echinochloa crus-galli (L.) Beauv.) or bearded sprangletop (sprangletop, Leptochloa fusca (L.) Kunth var. fascicularis (Lam.) N. Snow). The allelopathic cultivars produced more tillers and suppressed both weed species to a greater extent than did the breeding selections or the non-suppressive cultivars. C-13 isotope discrimination analysis of mixed root samples to a depth of 15 cm revealed that the allelopathic cultivars typically produced a greater fraction of their total root mass in the surface 0-5 cm of soil depth compared to the breeding selections or the non-suppressive cultivars, which tended to distribute their roots more evenly throughout the soil profile. These trends in root mass distribution were apparent at both early (pre-flood) and late-season stages in weed-free and weed-infested plots. Cultivar productivity and root distribution generally responded similarly to competition with the two weed species, but barnyardgrass reduced rice yield and root mass more than did sprangletop. These findings demonstrate for the first time that roots of the allelopathic cultivars PI 312777 and TN-1 explore the upper soil profile more thoroughly than do non-suppressive cultivars under weed-infested and weed-free conditions in flood-irrigated U.S. rice production systems. They raise the interesting prospect that root proliferation near the soil surface might enhance the weed-suppressive activity of allelochemical exudates released from roots. Plant architectural design for weed suppressive activity should take these traits into consideration along with other proven agronomic traits such as high tillering and yield. C1 [Gealy, David] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR 72160 USA. [Moldenhauer, Karen] Univ Arkansas, Div Agr, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Duke, Sara] ARS, USDA, Southern Plains Area, College Stn, TX USA. RP Gealy, D (reprint author), ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR 72160 USA. EM david.gealy@ars.usda.gov NR 65 TC 11 Z9 15 U1 1 U2 46 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2013 VL 39 IS 2 SI SI BP 186 EP 203 DI 10.1007/s10886-013-0246-7 PG 18 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 106BH UT WOS:000316115900005 PM 23397455 ER PT J AU Owens, DK Nanayakkara, NPD Dayan, FE AF Owens, Daniel K. Nanayakkara, N. P. Dhammika Dayan, Franck E. TI In planta Mechanism of Action of Leptospermone: Impact of Its Physico-Chemical Properties on Uptake, Translocation, and Metabolism SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Triketone; Phytotoxin; Mode of action; Allelopathy; Essential oil; Metabolism; Myrtaceae ID P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; BETA-TRIKETONES; NATURAL-PRODUCTS; ESSENTIAL OILS; ROOT UPTAKE; MANUKA OIL; MYRTACEAE; SCOPARIUM; HERBICIDE; DISCOVERY AB Leptospermone is a natural beta-triketone that specifically inhibits the enzyme p-hydrophyphenylpyruvate dioxygenase, the same molecular target site as that of the commercial herbicide mesotrione. The beta-triketone-rich essential oil of Leptospermum scoparium has both preemergence and postemergence herbicidal activity, resulting in bleaching of treated plants and dramatic growth reduction. Radiolabeled leptospermone was synthesized to investigate the in planta mechanism of action of this natural herbicide. Approximately 50 % of the absorbed leptospermone was translocated to the foliage suggesting rapid acropetal movement of the molecule. On the other hand, very little leptospermone was translocated away from the point of application on the foliage, indicating poor phloem mobility. These observations are consistent with the physico-chemical properties of leptospermone, such as its experimentally measured logP and pK (a) values, and molecular mass, number of hydrogen donors and acceptors, and number of rotatable bonds. Consequently, leptospermone is taken up readily by roots and translocated to reach its molecular target site. This provides additional evidence that the anecdotal observation of allelopathic suppression of plant growth under beta-triketone-producing species may be due to the release of these phytotoxins in soils. C1 [Owens, Daniel K.; Dayan, Franck E.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Nanayakkara, N. P. Dhammika] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 USA. RP Dayan, FE (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, POB 8048, University, MS 38677 USA. EM fdayan@olemiss.edu RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 NR 43 TC 11 Z9 11 U1 3 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2013 VL 39 IS 2 SI SI BP 262 EP 270 DI 10.1007/s10886-013-0237-8 PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 106BH UT WOS:000316115900011 PM 23314892 ER PT J AU Duke, SO Bajsa, J Pan, ZQ AF Duke, Stephen O. Bajsa, Joanna Pan, Zhiqiang TI Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Allelochemical; Herbicide; Metabolomics; Mode of action; Omics; Physionomics; Phytotoxin; Proteomics; Transcriptomics ID ARABIDOPSIS-THALIANA; GENE-EXPRESSION; TRANSCRIPTOME RESPONSE; PROTEIN PHOSPHATASE; PROTEOMIC ANALYSIS; PLANT-DISEASE; OF-ACTION; RNA-SEQ; HERBICIDE; PATHWAYS AB For a little over a decade, omics methods (transcriptomics, proteomics, metabolomics, and physionomics) have been used to discover and probe the mode of action of both synthetic and natural phytotoxins. For mode of action discovery, the strategy for each of these approaches is to generate an omics profile for phytotoxins with known molecular targets and to compare this library of responses to the responses of compounds with unknown modes of action. Using more than one omics approach enhances the probability of success. Generally, compounds with the same mode of action generate similar responses with a particular omics method. Stress and detoxification responses to phytotoxins can be much clearer than effects directly related to the target site. Clues to new modes of action must be validated with in vitro enzyme effects or genetic approaches. Thus far, the only new phytotoxin target site discovered with omics approaches (metabolomics and physionomics) is that of cinmethylin and structurally related 5-benzyloxymethyl-1,2-isoxazolines. These omics approaches pointed to tyrosine amino-transferase as the target, which was verified by enzyme assays and genetic methods. In addition to being a useful tool of mode of action discovery, omics methods provide detailed information on genetic and biochemical impacts of phytotoxins. Such information can be useful in understanding the full impact of natural phytotoxins in both agricultural and natural ecosystems. C1 [Duke, Stephen O.; Bajsa, Joanna; Pan, Zhiqiang] ARS, NPURU, USDA, University, MS 38677 USA. RP Duke, SO (reprint author), ARS, NPURU, USDA, POB 8048, University, MS 38677 USA. EM stephen.duke@ars.usda.gov NR 82 TC 10 Z9 10 U1 3 U2 56 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2013 VL 39 IS 2 SI SI BP 333 EP 347 DI 10.1007/s10886-013-0240-0 PG 15 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 106BH UT WOS:000316115900017 PM 23355015 ER PT J AU Wang, YQ Yang, Y Fei, ZJ Yuan, H Fish, T Thannhauser, TW Mazourek, M Kochian, LV Wang, XW Li, L AF Wang, Yong-Qiang Yang, Yong Fei, Zhangjun Yuan, Hui Fish, Tara Thannhauser, Theodore W. Mazourek, Michael Kochian, Leon V. Wang, Xiaowu Li, Li TI Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Carrot; cauliflower; chromoplast; papaya; pepper; proteomics; tomato; watermelon ID ARABIDOPSIS-THALIANA L; CAROTENOID ACCUMULATION; ANTIOXIDANT SYSTEM; POTATO-TUBERS; PROTEIN; CHLOROPLASTS; L.; PLASTIDS; IDENTIFICATION; DEHYDROGENASE AB Chromoplasts are unique plastids that accumulate massive amounts of carotenoids. To gain a general and comparative characterization of chromoplast proteins, this study performed proteomic analysis of chromoplasts from six carotenoid-rich crops: watermelon, tomato, carrot, orange cauliflower, red papaya, and red bell pepper. Stromal and membrane proteins of chromoplasts were separated by 1D gel electrophoresis and analysed using nLC-MS/MS. A total of 9532262 proteins from chromoplasts of different crop species were identified. Approximately 60% of the identified proteins were predicted to be plastid localized. Functional classification using MapMan bins revealed large numbers of proteins involved in protein metabolism, transport, amino acid metabolism, lipid metabolism, and redox in chromoplasts from all six species. Seventeen core carotenoid metabolic enzymes were identified. Phytoene synthase, phytoene desaturase, ?-carotene desaturase, 9-cis-epoxycarotenoid dioxygenase, and carotenoid cleavage dioxygenase 1 were found in almost all crops, suggesting relative abundance of them among the carotenoid pathway enzymes. Chromoplasts from different crops contained abundant amounts of ATP synthase and adenine nucleotide translocator, which indicates an important role of ATP production and transport in chromoplast development. Distinctive abundant proteins were observed in chromoplast from different crops, including capsanthin/capsorubin synthase and fibrillins in pepper, superoxide dismutase in watermelon, carrot, and cauliflower, and glutathione-S-transferease in papaya. The comparative analysis of chromoplast proteins among six crop species offers new insights into the general metabolism and function of chromoplasts as well as the uniqueness of chromoplasts in specific crop species. This work provides reference datasets for future experimental study of chromoplast biogenesis, development, and regulation in plants. C1 [Wang, Yong-Qiang; Yuan, Hui; Mazourek, Michael; Li, Li] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Yang, Yong; Fei, Zhangjun; Fish, Tara; Thannhauser, Theodore W.; Kochian, Leon V.; Li, Li] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Wang, Xiaowu] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China. RP Li, L (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. EM ll37@cornell.edu RI Yuan, Hui/H-6723-2016; OI Kochian, Leon/0000-0003-3416-089X; Wang, Yong-Qiang/0000-0001-9997-7636 FU USDA-ARS FX This work was supported by the USDA-ARS base fund. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 50 TC 31 Z9 33 U1 4 U2 76 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 2013 VL 64 IS 4 BP 949 EP 961 DI 10.1093/jxb/ers375 PG 13 WC Plant Sciences SC Plant Sciences GA 104OJ UT WOS:000316003600012 PM 23314817 ER PT J AU Kuhnle, RA Wren, DG Langendoen, EJ Rigby, JR AF Kuhnle, R. A. Wren, D. G. Langendoen, E. J. Rigby, J. R. TI Sand Transport over an Immobile Gravel Substrate SO JOURNAL OF HYDRAULIC ENGINEERING-ASCE LA English DT Article DE Sand; Gravel; Sediment transport ID DOUBLE-AVERAGING CONCEPT; BED OPEN-CHANNEL; ROUGH-BED; SEDIMENT TRANSPORT; OVERLAND FLOWS; TOPOGRAPHY; MIXTURES; VELOCITY; BEDLOAD; STREAMS AB Experiments were conducted in a laboratory flume channel to evaluate the effects of increasing amounts of sand on its transport over and through an immobile coarse gravel bed. Detailed measurements of sand transport rate, bed texture, and bed topography were collected for four different discharges at approximately the same flow depth of 0.2 m for 11 different elevations of sand in the gravel bed. Sand transport was measured using both physical samples and a density cell. For a given flow rate, increases in the elevation of sand relative to gravel resulted in decreases of bed shear stress from 32-44% and increases in sand transport by three orders of magnitude. For the highest two discharges, the sand merged into a small number of long and low bed forms that translated through and over the gravel bed. A collapse of the transport data was accomplished by relating the sand transport rate to the bed shear stress scaled by the cumulative probability distribution function of the gravel surface evaluated at the height of the mean sand bed. DOI: 10.1061/(ASCE)HY.1943-7900.0000615. (C) 2013 American Society of Civil Engineers. C1 [Kuhnle, R. A.; Wren, D. G.; Langendoen, E. J.; Rigby, J. R.] ARS, Natl Sedimentat Lab, USDA, Oxford, MS 38655 USA. RP Kuhnle, RA (reprint author), ARS, Natl Sedimentat Lab, USDA, POB 1157, Oxford, MS 38655 USA. EM roger.kuhnle@ars.usda.gov; daniel.wren@ars.usda.gov; eddy.langendoen@ars.usda.gov; jr.rigby@ars.usda.gov OI Rigby, James/0000-0002-5611-6307; Langendoen, Eddy/0000-0002-2215-4989 NR 46 TC 7 Z9 7 U1 0 U2 18 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 J9 J HYDRAUL ENG-ASCE JI J. Hydraul. Eng.-ASCE PD FEB PY 2013 VL 139 IS 2 BP 167 EP 176 DI 10.1061/(ASCE)HY.1943-7900.0000615 PG 10 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA 111YJ UT WOS:000316557800006 ER PT J AU Zhang, N Li, H AF Zhang, Na Li, Harbin TI Sensitivity and effectiveness and of landscape metric scalograms in determining the characteristic scale of a hierarchically structured landscape SO LANDSCAPE ECOLOGY LA English DT Article DE Hierarchically structured neutral landscape; Xilin River Basin of Inner Mongolia; FRAGSTATS; Class-level metric; Scale issues; Scale effect; Multi-scale pattern; Scaling ID PATTERN-ANALYSIS; CHANGING SCALE; ECOLOGY; MODELS AB Landscapemetric scalograms (the response curves of landscapemetrics to changing grain size) have been used to illustrate the scale effects of metrics for real landscapes. However, whether they detect the characteristic scale of hierarchically structured landscapes remains uncertain. To address this question, the scalograms of 26 class-level metrics were systematically examined for a simple random landscape, seven hierarchical neutral landscapes, and the real landscape of the Xilin River Basin of Inner Mongolia, China. The results show that when the fraction of the focal patch type (P) is below a critical value (P-c), most metric scalograms are sensitive to change in single-scale and lower-level hierarchical structure and insensitive to change in higher-level hierarchical structure. The scalograms of only a few metrics measuring spatial aggregation and connectedness are sensitive to change in intermediate-level hierarchical structure. Most metric scalograms explicitly identify the characteristic scale of a single-scale landscape and fine or intermediate characteristic scales of a multi-scale landscape for both simulated and real landscapes. When P exceeds P-c, only some metrics detect scale and change in structure. The scalograms of total class area and Euclidean nearest-neighbor distance cannot detect scale or change in structure in either case. Landscape metric scalograms are useful for addressing scale issues, including illustrating the scale effects of spatial patterns, detecting multi-scale patterns, and developing possible scaling relations. C1 [Zhang, Na] Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China. [Li, Harbin] US Forest Serv, USDA, Southern Res Stn, Ctr Forested Wetlands Res, Charleston, SC 29414 USA. RP Zhang, N (reprint author), Univ Chinese Acad Sci, Coll Resources & Environm, 19A Yu Quan Rd, Beijing 100049, Peoples R China. EM zhangna@ucas.ac.cn; hli@fs.fed.us FU National Natural Science Foundation of China [31270512, 30870430]; Key Topics in Innovation Engineering of Chinese Academy of Sciences (CAS) [Y225016EA2] FX The study was supported by the National Natural Science Foundation of China (31270512; 30870430), and the Key Topics in Innovation Engineering of Chinese Academy of Sciences (CAS) (Y225016EA2). We thank the Chinese Ecosystem Research Network and Inner Mongolia Grassland Ecosystem Research Station of the CAS for providing the vegetation map of the Xilin River Basin. We also gratefully acknowledge the editor Santiago Saura and three anonymous reviewers for their valuable comments and constructive suggestions. NR 34 TC 4 Z9 4 U1 4 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2013 VL 28 IS 2 BP 343 EP 363 DI 10.1007/s10980-012-9837-x PG 21 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA 108JT UT WOS:000316289800013 ER PT J AU DeLaune, PB Moore, PA AF DeLaune, P. B. Moore, P. A., Jr. TI 17 beta-estradiol in runoff as affected by various poultry litter application strategies SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE 17 beta-estradiol; Non-point source; Runoff; Poultry litter ID 17 BETA-ESTRADIOL; REDUCING PHOSPHORUS RUNOFF; AGRICULTURAL SOILS; ESTROGENIC HORMONES; BROILER LITTER; ALUMINUM SULFATE; STEROID-HORMONES; UNITED-KINGDOM; FECAL BACTERIA; RAINBOW-TROUT AB Steroidal hormones, which are excreted by all mammalian species, have received increasing attention in recent years due to potential environmental implications. The objective of this study was to evaluate 17 beta-estradiol concentrations in runoff water from plots receiving poultry litter applications using various management strategies. Treatments included the effects of 1) aluminum sulfate (alum) application rates to poultry litter; 2) time until the first runoff event occurs after poultry litter application; 3) poultry litter application rate; 4) fertilizer type; and 5) litter from birds fed modified diets. Rainfall simulators were used to cause continuous runoff from fertilized plots. Runoff samples were collected and analyzed for 17 beta-estradiol concentrations. Results showed that increasing alum additions to poultry litter decreased 17 beta-estradiol concentrations in runoff water. A significant exponential decline in 17 beta-estradiol runoff was also observed with increasing time until the first runoff event after litter application. Concentrations of 17 beta-estradiol in runoff water increased with increasing litter application rate and remained above background concentrations after three runoff events at higher application rates. Management practices such as diet modification and selection of fertilizer type were also shown to affect 17 beta-estradiol concentrations in runoff water. Although results from these experiments typically represented a worst case scenario since runoff events generally occurred immediately after litter application, the contaminant loss from pastures fertilized with poultry litter can be expected to be much lower than continual estradiol loadings observed from waste water treatment plants. Management practices such as alum amendment and application timing can significantly reduce the risk of 17 beta-estradiol losses in the environment. (C) 2012 Elsevier B.V. All rights reserved. C1 [DeLaune, P. B.] Texas A&M AgriLife Res, Vernon, TX 76385 USA. [Moore, P. A., Jr.] Univ Arkansas, USDA ARS, PPSRU, Fayetteville, AR 72701 USA. RP DeLaune, PB (reprint author), Texas A&M AgriLife Res, POB 1658, Vernon, TX 76385 USA. EM pbdelaune@ag.tamu.edu NR 57 TC 7 Z9 7 U1 0 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2013 VL 444 BP 26 EP 31 DI 10.1016/j.scitotenv.2012.11.054 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA 107SD UT WOS:000316240200003 PM 23262322 ER PT J AU Funnell-Harris, DL Prom, LK Pedersen, JF AF Funnell-Harris, Deanna L. Prom, Louis K. Pedersen, Jeffrey F. TI Isolation and characterization of the grain mold fungi Cochliobolus and Alternaria spp. from sorghum using semiselective media and DNA sequence analyses SO CANADIAN JOURNAL OF MICROBIOLOGY LA English DT Article DE Alternaria; Cochliobolus; Fusarium; semiselective media; Sorghum bicolor ID FUSARIUM-THAPSINUM; ROOT-ROT; MOLECULAR CHARACTERIZATION; BIPOLARIS-SOROKINIANA; CURVULARIA-LUNATA; INFECTION; PATHOGENS; THEMES; GROWTH; DIFFERENTIATION AB Mold diseases, caused by fungal complexes including Alternaria, Cochliobolus, and Fusarium species, limit sorghum grain production. Media were tested by plating Fusarium thapsinum, Alternaria sp., and Curvularia lunata, individually and competitively. Dichloran chloramphenicol rose bengal (DRBC) and modified V8 juice (ModV8) agars, found to be useful, were compared with commonly used agar media, dichloran chloramphenicol peptone (DCPA) and pentachloronitrobenzene (PCNB). Radial growth, starting with mycelia or single-conidia and hyphal tips, demonstrated an effect of media. For isolation of grain fungi, DRBC and ModV8 were similar or superior to DCPA and PCNB. When seedlings were inoculated with conidia of C. lunata, Alternaria sp., F. thapsinum, or mixtures, the percentage of root infection ranged from 28% to 77%. For mixed inoculations, shoot weights, lesion lengths, and percentage of root infections were similar to F. thapsinum inoculations; most colonies recovered from roots were F. thapsinum. For Alternaria grain isolates, 5 morphological types, including Alternaria alternata, were distinguished by colony morphologies and conidial dimensions. Sequence analysis using a portion of the endo-polygalacturonase gene was able to further distinguish isolates. Cochliobolus isolates were identified morphologically as C. lunata, Curvularia sorghina, and Bipolaris sorghicola. Multiple molecular genotypes were apparent from rRNA internal transcribed spacer region sequences from Cochliobolus grain isolates. C1 [Funnell-Harris, Deanna L.; Pedersen, Jeffrey F.] Univ Nebraska, Dept Plant Pathol, ARS, USDA,Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Prom, Louis K.] ARS, USDA, So Plains Agr Res Ctr, Crop Germplasm Res Unit, College Stn, TX 77845 USA. RP Funnell-Harris, DL (reprint author), Univ Nebraska, Dept Plant Pathol, ARS, USDA,Grain Forage & Bioenergy Res Unit, 137 Keim Hall,UNL East Campus, Lincoln, NE 68583 USA. EM Deanna.Funnell-Harris@ars.usda.gov NR 59 TC 6 Z9 6 U1 0 U2 21 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0008-4166 EI 1480-3275 J9 CAN J MICROBIOL JI Can. J. Microbiol. PD FEB PY 2013 VL 59 IS 2 BP 87 EP 96 DI 10.1139/cjm-2012-0649 PG 10 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology GA 103OX UT WOS:000315929300003 PM 23461515 ER PT J AU Washburn, BE Bernhardt, GE Kutschbach-Brohl, L Chipman, RB Francoeur, LC AF Washburn, Brian E. Bernhardt, Glen E. Kutschbach-Brohl, Lisa Chipman, Richard B. Francoeur, Laura C. TI FORAGING ECOLOGY OF FOUR GULL SPECIES AT A COASTAL-URBAN INTERFACE SO CONDOR LA English DT Article DE Charadriiformes; dietary analysis; foraging; gulls; Larus; urban ID BLACK-BACKED GULLS; LAUGHING GULLS; HERRING-GULLS; NEW-YORK; FOOD-HABITS; TEMPORAL VARIATION; LARUS-ARGENTATUS; STOMACH CONTENTS; FEEDING ECOLOGY; WESTERN GULL AB Coastal urban environments provide a potentially diverse source of food for gulls, including items of marine, terrestrial, and anthropogenic origin. Our objective was to examine variation in the diet and use of feeding habitat of four species of gulls, the Laughing (Leucophaeus atricilla), Herring (Larus argentatus), Great Black-backed (L. marinus), and Ring-billed (L. delawarensis), at a coastal urban interface. We necropsied, identified the sex and age class, and quantified the stomach contents of 1053 Laughing, 249 Herring, 67 Great Black-backed, and 31 Ring-billed Gulls collected near the New York City metropolitan area in 2003 and 2004. Great Black-backed Gulls specialized on marine foods, whereas Ring-billed Gulls were generalists. Laughing Gulls and Herring Gulls favored marine foods and foraged in marine habitats but also used terrestrial and anthropogenic food sources. We found evidence that demographics influenced the gulls' choice of diet and use of feeding habitat. Laughing Gulls and Herring Gulls switched their use of feeding habitats at various stages of breeding, exploiting :terrestrial prey and feeding habitats most during chick rearing. Interspecific and intraspecific differences in the four species' diet and use of feeding habitat apparently allow for their coexistence at this coastal-urban interface. C1 [Washburn, Brian E.; Bernhardt, Glen E.; Kutschbach-Brohl, Lisa] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Chipman, Richard B.] USDA, Wildlife Serv, Concord, NH 03301 USA. [Francoeur, Laura C.] Port Authority NY & NJ, John F Kennedy Int Airport, Jamaica, NY 11430 USA. RP Washburn, BE (reprint author), USDA, Wildlife Serv, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU PANYNJ; USDA/APHIS/WS FX We thank S. Nowak of the Port Authority of New York and New Jersey (PANYNJ) and A. Gosser, D. Sullivan, K. Pruesser, D. Helon, S. Johnston, and others from the U.S. Department of Agriculture, Animal Plant Health Inspection Service, Wildlife Services (USDA/APHIS/WS) for their assistance with field and laboratory procedures. T. DeVault, B. Blackwell, and T. Seamans, and two anonymous reviewers kindly provided helpful comments on earlier versions of the manuscript. Financial and logistical support for this project was provided by the PANYNJ and USDA/APHIS/WS. This research was approved by the National Wildlife Research Center's Institutional Animal Care and Use Committee (QA-1057) and conducted under appropriate state and federal permits. NR 74 TC 8 Z9 10 U1 6 U2 81 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 J9 CONDOR JI Condor PD FEB PY 2013 VL 115 IS 1 BP 67 EP 76 DI 10.1525/cond.2013.110185 PG 10 WC Ornithology SC Zoology GA 099XS UT WOS:000315659800009 ER PT J AU Wolfe, JD Johnson, MD Ralph, CJ AF Wolfe, Jared D. Johnson, Matthew D. Ralph, C. John TI GREATER MASS INCREASES ANNUAL SURVIVAL OF PROTHONOTARY WARBLERS WINTERING IN NORTHEASTERN COSTA RICA SO CONDOR LA English DT Article DE Costa Rica; migrant; survival; population growth; mass; over-winter ID HABITAT QUALITY; MIGRATORY BIRD; POPULATION-DYNAMICS; SOUTHERN VERACRUZ; WOOD THRUSH; TEMPERATE; RATES; SONGBIRD; FORESTS; CYCLE AB Estimates of survival of nearctic-neotropic migrants have broadened our understanding of life-history variation across taxa and latitudes. Despite the importance of assessing migrants' survival through all phases of their life-cycle, data from their tropical winter ranges are few. In this study we used 14 years of data on captured birds to quantify the influence of mass on the annual survival of the Prothonotary Warbler (Protonotaria citrea) in northeastern Costa Rica and compared our results to survival estimates generated from the breeding grounds. Furthermore, from estimates of population growth (X) based on marked individuals, we projected the Prothonotary Warbler's demographic trajectory at our study site. Our results suggest that heavier individuals had higher rates of annual survival. Population growth at our study sites was stable, punctuated by swings in growth and decline. We believe the benefits of weight gain (preparation for migration and intraspecific competition for territories) outweigh inherent costs (greater susceptibility to predation). C1 [Wolfe, Jared D.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.; Johnson, Matthew D.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Wolfe, Jared D.; Ralph, C. John] USDA, Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. [Wolfe, Jared D.; Ralph, C. John] Klamath Bird Observ, Ashland, OR 97520 USA. RP Wolfe, JD (reprint author), Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM jwolfe5@lsu.edu NR 33 TC 4 Z9 4 U1 2 U2 27 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD FEB PY 2013 VL 115 IS 1 BP 163 EP 167 DI 10.1525/cond.2012.120084 PG 5 WC Ornithology SC Zoology GA 099XS UT WOS:000315659800018 ER PT J AU Sparger, JA Norton, GW Heisey, PW Alwang, J AF Sparger, John Adam Norton, George W. Heisey, Paul W. Alwang, Jeffrey TI Is the share of agricultural maintenance research rising in the United States? SO FOOD POLICY LA English DT Article DE Agricultural maintenance research; Expenditures; Productivity; Policy ID PRODUCTIVITY; UNIVERSITIES; RETURNS AB Public research is a major contributor to agricultural productivity growth, but if research investments are not maintained, agricultural productivity can decline over time. Maintenance research replaces deteriorated research knowledge to forestall a productivity decline. Knowledge of the magnitude of maintenance research can facilitate a more complete assessment of the value of agricultural research programs. Trends in maintenance research and sources of change in those trends are investigated. Results indicate that overall, about 40% of US agricultural research is devoted to maintenance, up from about a third 25 years ago. A model is developed and estimated to explain maintenance research expenditures. Research funding, climatic conditions, insect and pathogen control, and agricultural production choices influence maintenance research expenditures. Increased reliance on out-of-state funding sources may skew agricultural research away from maintenance research, while climate change may increase the need for such research. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Sparger, John Adam; Norton, George W.; Alwang, Jeffrey] Virginia Tech, Dept Agr & Appl Econ, Blacksburg, VA 24061 USA. [Heisey, Paul W.] ERS, USDA, Washington, DC 20024 USA. RP Norton, GW (reprint author), Virginia Tech, Dept Agr & Appl Econ, 2056 Hutcheson Hall 0401, Blacksburg, VA 24061 USA. EM gnorton@vt.edu FU US Department of Agriculture, Economic Research Service FX The authors would like to thank the US Department of Agriculture, Economic Research Service for financial support, and Chris Parameter and two anonymous reviewers for helpful comments. The views in this article are those of the authors and do not necessarily reflect official policy of ERS or the USDA. NR 25 TC 2 Z9 3 U1 2 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 J9 FOOD POLICY JI Food Policy PD FEB PY 2013 VL 38 BP 126 EP 135 DI 10.1016/j.foodpol.2012.11.004 PG 10 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA 098MP UT WOS:000315553700012 ER PT J AU Rada, N AF Rada, Nicholas TI Assessing Brazil's Cerrado agricultural miracle SO FOOD POLICY LA English DT Article DE Cerrado; Brazilian agriculture; Input distance function; Stochastic frontier; Total factor productivity; Technical change; Efficiency ID EFFICIENCY AB Brazil's emergence as a primary global agricultural producer is often credited to production expansion into soils of the Brazilian savannah or Cerrado. These soils are, however, deficient in important nutrients and prone to degradation, requiring input-intensive processes that suggest a low level of productive efficiency. Employing a sequence of agricultural censuses and a biome approach for characterizing agricultural zones, the present study evaluates the Cerrado's total factor productivity growth and productive potential. The analysis highlights the resource cost of Brazil's "Cerrado Miracle," the role of paved road infrastructure in expanding production opportunities, and the significant production gains that the Cerrado may yet achieve. Results suggest a substantial productivity gap between the Cerrado's most efficient and average producers, implying that Cerrado production might well be further boosted if average producers succeed in adopting the technologies and management practices of the more efficient operators. More generally, and to the extent the Cerrado model is generalizable elsewhere, agricultural development of the world's savannahs, such as Sub-Saharan Africa's Guinea regions, into breadbaskets will be expensive in terms of material inputs such as fertilizers and pesticides, depending for their success therefore on the real prices of these inputs. Published by Elsevier Ltd. C1 [Rada, Nicholas] ERS, Food Secur & Dev Branch, Market & Trade Econ Div, USDA, Washington, DC 20024 USA. RP Rada, N (reprint author), ERS, USDA, 355 E St SW,6-269B, Washington, DC 20024 USA. EM nrada@ers.usda.gov NR 40 TC 15 Z9 15 U1 0 U2 36 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 J9 FOOD POLICY JI Food Policy PD FEB PY 2013 VL 38 BP 146 EP 155 DI 10.1016/j.foodpol.2012.11.002 PG 10 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA 098MP UT WOS:000315553700014 ER PT J AU Fettig, CJ Burnside, RE Hayes, CJ Kruse, JJ Lisuzzo, NJ McKelvey, SR Mori, SR Nickel, SK Schultz, ME AF Fettig, Christopher J. Burnside, Roger E. Hayes, Christopher J. Kruse, James J. Lisuzzo, Nicholas J. McKelvey, Stephen R. Mori, Sylvia R. Nickel, Stephen K. Schultz, Mark E. TI Factors influencing northern spruce engraver colonization of white spruce slash in interior Alaska SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuels reduction; Forest restoration; Ips perturbatus; Picea glauca; Semiochemical; Slash management ID PONDEROSA PINE SLASH; DENDROCTONUS-BREVICOMIS COLEOPTERA; BARK BEETLES; IPS-PERTURBATUS; NONHOST ANGIOSPERMS; BAITED TRAPS; CONE BEETLE; LOG SIZE; SCOLYTIDAE; VOLATILES AB In interior Alaska, increased use of mechanical fuel reduction treatments, increased interests in the use of wood energy systems as alternatives to fossil fuels, and elevated populations of northern spruce engraver, Ips perturbatus (Eichhoff), have raised concerns regarding the impact of this bark beetle to forest resources. We conducted a large-scale field study in 2009-2011 (Study 1) to determine the effects of slash scoring (mechanical by chainsaw versus none), slash distribution (scattered versus decked), and cutting date (spring versus fall) on I. perturbatus colonization of and reproductive performance in white spruce, Picea glauca (Moench) Voss, slash, and to determine the effects of resulting treatments on adjacent levels of tree mortality caused by I. perturbatus. Unfortunately, attack densities were lower than expected, and did not provide for a very robust examination of the effects of these treatments. As a result, we reproduced several aspects of Study 1 in a second study (2011) using a baited design. Higher levels of I. perturbatus attack and emergence occurred on dispersed logs. Attack densities were highest in the dispersed, unscored treatment, and similar to 70% higher than observed in the decked, scored treatment. The scoring of dispersed logs significantly reduced attack densities by similar to 28%, but had no effect in decked treatments or on levels of emergence in either treatment. Higher levels of attack and emergence were observed on the tops of logs as compared to the bottoms of logs. Brood production (i.e., defined here as emergence/attacks) was also greater on the tops of logs compared to the bottoms of logs, suggesting the tops of logs are not only more attractive to I. perturbatus, but confer some advantage to brood development. Lower levels of attack and emergence occurred on small diameter logs. Higher levels of attack and emergence were observed on logs in a shaded fuelbreak (i.e., a more open condition of lower tree density) compared to the adjacent forest. Overall, our research suggests that unlike other works on Ips spp. in the western USA that promote the desiccation of slash to minimize colonization and brood production, I. perturbatus appears regulated by the apparency and accessibility of host material. This finding highlights the importance of developing management guidelines based on local science. A third study found two semiochemicals, trans-conophthorin and verbenone, reduced colonization of slash by I. perturbatus, and therefore holds promise as a tool for managing I. perturbatus populations. The implications of these and other results to the management of I. perturbatus in interior Alaska are discussed. C1 [Fettig, Christopher J.; McKelvey, Stephen R.] USDA, Pacific Southwest Res Stn, Forest Serv, Davis, CA 95618 USA. [Burnside, Roger E.] State Alaska, Forest Hlth Protect Program, Dept Nat Resources, Div Forestry, Anchorage, AK 99501 USA. [Hayes, Christopher J.] USDA, Forest Serv, Missoula, MT 59802 USA. [Kruse, James J.; Lisuzzo, Nicholas J.] USDA, Forest Serv, Fairbanks, AK 99709 USA. [Mori, Sylvia R.] USDA, Pacific Southwest Res Stn, Forest Serv, Berkeley, CA 94710 USA. [Nickel, Stephen K.] State Alaska, Community Forestry Program, Dept Nat Resources, Div Forestry, Anchorage, AK 99501 USA. RP Fettig, CJ (reprint author), USDA, Pacific Southwest Res Stn, Forest Serv, 1731 Res Pk Dr, Davis, CA 95618 USA. EM cfettig@fs.fed.us FU USDA Forest Service Special Technology Development Program grant [R10-2009-01]; USDA Forest Service; U.S. President's Early Career Award for Scientists and Engineers; Alaska Department of Natural Resources, Forest Health Protection (USDA Forest Service); Pacific Southwest Research Station (USDA Forest Service) FX We thank J. Hermanns and J. Douse (Alaska Department of Natural Resources), and D. Rees (Colorado State University) for providing access to study locations. We thank D. Cluck, C. Dabney and C. Frank (USDA Forest Service), M. Sprau and E. Usborne (U.S. Army), W. Upton (Texas Forest Service) and D. Rees for technical assistance. We thank A. Graves, S. Patterson and S. Seybold (USDA Forest Service), and R. Ott (RAO Ecological Consulting) for helpful insights. A special thanks to C. Dabney (USDA Forest Service) for his critique, which improved an earlier version of this manuscript. This work was supported, in part, by a USDA Forest Service Special Technology Development Program grant (R10-2009-01) to R.E.B., M.E.S., J.J.K., and J. Lundquist (USDA Forest Service), a U.S. President's Early Career Award for Scientists and Engineers to C.J.F., Alaska Department of Natural Resources, Forest Health Protection (USDA Forest Service), and the Pacific Southwest Research Station (USDA Forest Service). NR 60 TC 2 Z9 2 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 58 EP 68 DI 10.1016/j.foreco.2012.09.040 PG 11 WC Forestry SC Forestry GA 099XP UT WOS:000315659500008 ER PT J AU Morris, JL le Roux, PC Macharia, AN Brunelle, A Hebertson, EG Lundeen, ZJ AF Morris, Jesse L. le Roux, Peter C. Macharia, Anthony N. Brunelle, Andrea Hebertson, Elizabeth G. Lundeen, Zachary J. TI Organic, elemental, and geochemical contributions to lake sediment deposits during severe spruce beetle (Dendroctonus rufipennis) disturbances SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE C/N ratio; Spruce beetle; Dendroctonus rufipennis; GLMM; Disturbance; Lake sediments ID WESTERN UNITED-STATES; SUB-ALPINE FORESTS; CONIFEROUS FORESTS; MARKAGUNT PLATEAU; NORTH-AMERICA; NATIONAL-PARK; BARK BEETLES; PINE FORESTS; ICE-AGE; USA AB Recent outbreaks of native bark beetles are unprecedented during the historical period. The aim of this manuscript is to develop a proxy-based methodology to infer past bark beetle outbreaks using lake sediments to provide long-term context for recent outbreaks. We test three hypotheses to determine how the ecological impacts of severe spruce beetle (Dendroctonus rufipennis Kirby) disturbances are recorded in lake sediment deposits. The resulting mortality and defoliation of Engelmann spruce is hypothesized to: (1) decrease the ratio of spruce to fir pollen; (2) reduce canopy interception of precipitation leading to an increase in soil erosion and/or enhanced mobilization of terrestrial carbon; and (3) leach foliar nitrogen and enhance algal productivity resulting in increased nitrogen values in lake sediments. To test these hypotheses, we analyzed sediment cores from six spruce beetle-affected basins in Utah for spruce/fir pollen ratio (hypothesis 1), loss-on-ignition, magnetic susceptibility of sediments (hypothesis 2), and 8(13)C(BOM), 8(15)N(TN), elemental C and N, and the C/N ratio of bulk organic material (hypotheses 2 and 3). The dataset was statistically tested using general linear mixed models (GLMMs) to determine if the response variables differed significantly between outbreak and non-outbreak period. The spruce/fir pollen ratio responded significantly to outbreaks at all sites suggesting that this metric may be the most suitable for identifying past spruce beetle outbreaks. For our second hypothesis we found little support for an influx of terrestrial C due to strongly individualist responses of the basins. For our third hypotheses we found little support for increased sedimentary nitrogen, likely due to alterations to nutrient cycling from human activities. Therefore the host/non-host pollen ratio provides the most promising metric for detecting past outbreaks. (C) 2012 Elsevier B.V. All rights reserved. C1 [Morris, Jesse L.; le Roux, Peter C.] Univ Helsinki, Dept Geosci & Geog, FIN-00014 Helsinki, Finland. [Macharia, Anthony N.; Brunelle, Andrea; Lundeen, Zachary J.] Univ Utah, Dept Geog, Salt Lake City, UT USA. [Hebertson, Elizabeth G.] USDA, Forest Serv, Forest Hlth Protect Ogden Field Off, Ogden, UT 84403 USA. [Lundeen, Zachary J.] Univ Utah, Rio Mesa Ctr, Salt Lake City, UT 84112 USA. RP Morris, JL (reprint author), Univ Helsinki, Dept Geosci & Geog, Gustaf Hallstromin Katu 2A, FIN-00014 Helsinki, Finland. EM jesse.morris@helsinki.fi RI le Roux, Peter/E-7784-2011 OI le Roux, Peter/0000-0002-7941-7444 FU Geological Society of America, Association of American Geographers; National Science Foundation [1032099]; Joint Fire Science Program [063131] FX This research was funded by awards from the Geological Society of America, Association of American Geographers, and the National Science Foundation (1032099) to Jesse Morris and by a grant from the Joint Fire Science Program (063131) to Andrea Brunelle. We thank Jennifer Watt for beneficial discussions and A. Steve Munson for sharing photographs. We extend our gratitude to Brad Erkkila at SIRFER Lab (University of Utah) for consultation on stable isotope analyses. This manuscript benefitted from discussions with John Marshall and Steve Perakis at the Paleo Reconstructions of Biogeochemical Environments (PROBE) Workshop at Kansas State University in Manhattan, Kansas (April 19-21, 2012). We are grateful to Hubert Sterba and two anonymous reviewers for providing comments and suggestions that greatly improved our manuscript. NR 84 TC 8 Z9 10 U1 0 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 78 EP 89 DI 10.1016/j.foreco.2012.10.004 PG 12 WC Forestry SC Forestry GA 099XP UT WOS:000315659500010 ER PT J AU Trager, MD Ristau, TE Stoleson, SH Davidson, RL Acciavatti, RE AF Trager, Matthew D. Ristau, Todd E. Stoleson, Scott H. Davidson, Robert L. Acciavatti, Robert E. TI Carabid beetle responses to herbicide application, shelterwood seed cut and insect defoliator outbreaks SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carabidae; Defoliation; Forest; Geometridae; Herbicide; Shelterwood ID NORTHERN HARDWOOD FOREST; 10-YEAR RESPONSE; BOREAL FOREST; GYPSY-MOTH; COLEOPTERA; ASSEMBLAGES; LEPIDOPTERA; BIODIVERSITY; LYMANTRIIDAE; COMMUNITIES AB Managing forests to promote biodiversity requires understanding the effects of silvicultural practices on a range of forest species and communities. We evaluated carabid beetle (Coleoptera; Carabidae) responses to operational herbicide and shelterwood seed cut treatments in northern hardwood stands on the Allegheny National Forest, Pennsylvania, USA, from 1992 to 2000. There was substantial defoliation by elm span-worms (Ennomos subsignarius Hubner) or cherry scallopshell moths (Hydria prunivorata Ferguson) during four of the 9 years of this study, so we also evaluated effects of these lepidopteran outbreaks on carabids. We found no differences in carabid species richness between herbicide-treated and no herbicide plots overall or in any year, but carabid abundance was higher in herbicide-treated plots in the year following application. Carabid community composition differed among years and increased in dissimilarity over the course of the study but did not differ between herbicide-treated and no herbicide plots. Shelterwood seed cuts had no effects on carabid species richness, abundance or community composition. The relatively few significant effects of experimental treatments on individual carabid species tended to be small and responses we did find differed somewhat from previous studies. In 1992, carabid abundance was significantly correlated with elm spanworm defoliation and in 1995 both species richness and abundance were significantly higher in areas defoliated by cherry scallopshell moth. These results support previous findings that forestry practices that have relatively minor and short-term effects on forest vegetation are unlikely to have substantial effects on carabids; however, natural resource variation resulting from forest lepidopteran outbreaks may have important cascading effects for carabid communities that have not been fully explored. Published by Elsevier B.V. C1 [Trager, Matthew D.] USDA, Forest Serv, Natl Forests Florida, Tallahassee, FL 32308 USA. [Trager, Matthew D.; Ristau, Todd E.; Stoleson, Scott H.] USDA, Forest Serv, No Res Stn, Irvine, PA 16329 USA. [Davidson, Robert L.; Acciavatti, Robert E.] Carnegie Museum Nat Hist, Sect Invertebrate Zool, Pittsburgh, PA 15213 USA. RP Trager, MD (reprint author), USDA, Forest Serv, Natl Forests Florida, 325 John Knox Rd Ste F-100, Tallahassee, FL 32308 USA. EM mdtrager@fs.fed.us; tristau@fs.fed.us; sstoleson@fs.fed.us; davidsonr@carnegiemnh.org FU Forest Service Pesticide Impact Assessment Program (FS-PIAP) FX We thank the Forest Service Pesticide Impact Assessment Program (FS-PIAP) for funding this work, the Allegheny National Forest staff for helping identify sites and implementing the fencing, cutting, and herbicide treatments. David deCalesta and Stephen Horsley designed the study and David Turner of Turner Enterprises, Youngsville, PA applied the herbicide. Julie Smithbauer, Linda Ordiway, and several seasonal technicians collected the carabid samples. Alex Royo, Michael Ulyshen, James Hanula and two anonymous reviewers provided helpful comments on this manuscript. NR 54 TC 3 Z9 3 U1 0 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 269 EP 277 DI 10.1016/j.foreco.2012.10.025 PG 9 WC Forestry SC Forestry GA 099XP UT WOS:000315659500029 ER PT J AU Hubbard, RM Rhoades, CC Elder, K Negron, J AF Hubbard, Robert M. Rhoades, Charles C. Elder, Kelly Negron, Jose TI Changes in transpiration and foliage growth in lodgepole pine trees following mountain pine beetle attack and mechanical girdling SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Blue stain fungi; Phloem; Sap flow; Pinus contorta; Dendroctonus ponderosae ID BLUE-STAIN FUNGI; HEAT PULSE VELOCITY; LOBLOLLY-PINE; BARK BEETLES; DENDROCTONUS-PONDEROSAE; ECOLOGICAL IMPLICATIONS; RANGE EXPANSION; BOREAL FOREST; NORTH-AMERICA; WOODY-PLANTS AB The recent mountain pine beetle outbreak in North American lodgepole pine forests demonstrates the importance of insect related disturbances in changing forest structure and ecosystem processes. Phloem feeding by beetles disrupts transport of photosynthate from tree canopies and fungi introduced to the tree's vascular system by the bark beetles inhibit water transport from roots to canopy; the implications of these processes for tree mortality are poorly understood. We hypothesized that the fungus must quickly disrupt tree water relations because phloem girdling, reported in other studies, requires much longer than a year to cause mortality. We tested the hypothesis in iodgepole pine (Pinus contorta) by comparing tree water use, foliar expansion and seasonal variation in predawn water potential on 8 mechanically girdled trees, 10 control trees and 17 trees attacked by mountain pine beetle (Dendroctonous ponderosae). Transpiration began to decline within ten days of beetle infestation; two months later, pre-dawn water potential had also dropped significantly as water transport to the canopy declined by 60% relative to healthy trees. There was no water transport or foliar expansion by beetle-infested trees the following year. Experimentally girdled trees continued to transpire, maintain leaf water potential and grow new foliage similar to healthy trees. Our data suggest that fungi introduced by bark beetles in this study are the primary cause of tree mortality following mountain pine beetle attack and significantly reduce transpiration soon after beetle infestation. Rapid decline and the eventual cessation of water uptake by infected trees have important implications for water and nutrient cycling in beetle impacted forests. Published by Elsevier 13.V. C1 [Hubbard, Robert M.; Rhoades, Charles C.; Elder, Kelly; Negron, Jose] USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Hubbard, RM (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80526 USA. EM rhubbard@fs.fed.us RI Hubbard, Robert/J-6059-2012 FU United States Department of Agriculture Forest Service, Rocky Mountain Research Station FX The authors thank Scott Baggett for advice with statistical analyses, Richard Waring, Michael Ryan, Dan Binkley, William Jacobi and one anonymous reviewer for their helpful comments on the manuscript, and Daniel Beverly for field assistance. Funding for this research was provided by the United States Department of Agriculture Forest Service, Rocky Mountain Research Station. NR 48 TC 36 Z9 36 U1 5 U2 98 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 312 EP 317 DI 10.1016/j.foreco.2012.09.028 PG 6 WC Forestry SC Forestry GA 099XP UT WOS:000315659500033 ER PT J AU Bryars, C Maier, C Zhao, DH Kane, M Borders, B Will, R Teskey, R AF Bryars, Charles Maier, Chris Zhao, Dehai Kane, Michael Borders, Bruce Will, Rodney Teskey, Robert TI Fixed physiological parameters in the 3-PG model produced accurate estimates of loblolly pine growth on sites in different geographic regions SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Hybrid process model; 3-PG(lob); Lob lolly pine; Parameterization ID ASSESSING FOREST PRODUCTIVITY; SOUTHERN UNITED-STATES; NET PRIMARY PRODUCTION; TAEDA L.; EUCALYPTUS PLANTATION; CARBON ALLOCATION; NEW-ZEALAND; NUTRIENT MANIPULATIONS; ELLIOTTII STANDS; STEM GROWTH AB The goal of this project was to determine if 3-PG could accurately predict growth of loblolly pine (Pinus taeda) plantations across a range of sites in the Piedmont and Coastal Plain provinces of Georgia using a fixed physiological parameter set. The model was slightly modified to improve predictions of volume, mortality and initial biomass growth. We hypothesized that because: (a) many physiological attributes of loblolly pine including rates of net photosynthesis, dark respiration, stomatal conductance and specific leaf area tend to be very similar across sites, and (b) leaf area is highly responsive to fertility but less so to water and other environmental factors, a single physiological parameter set would be suitable for predicting growth across a range of loblolly pine plantations which differed in soil type, climate, and silvicultural treatments. The parameter set was obtained from a combination of published values in the literature and model calibrations developed from a single highly productive stand in the Coastal Plain province in Georgia. Differences in potential productivity among sites were accounted for by only changing the value of the fertility rating and the soil type. The calibrated model was evaluated using observed growth data obtained from a slower growing stand at the same Coastal Plain site as the calibration stand, and three other sites, two in the Piedmont province and one in the Coastal Plain. At each of the four locations the model was evaluated using growth data from two plantations, one that received minimal silvicultural inputs and another that received a highly intensive silvicultural treatment, including complete and sustained competition control and annual fertilization. The model performed well on all stands and treatments, and accurately estimated stem biomass and diameter growth. The percent average deviation between predicted and observed values was less than 20% on most sites. The model did not accurately predict stand density in some cases and tended to overestimate volume on sites with low intensity silviculture. The inaccuracy in predicting volume was attributed to parameterization on a highly fertilized site and an inability to account for fertilization effects on wood density. Despite the discrepancies in measured and modeled stem density and volume it is our conclusion that, overall, the 3-PG model provided an accurate description of loblolly pine plantation growth and productivity and was a particularly good predictor of stand biomass in both the Piedmont and Coastal Plain provinces of the Southeastern US using a single set of physiological parameters. (C) 2012 Elsevier B.V. All rights reserved. C1 [Bryars, Charles; Zhao, Dehai; Kane, Michael; Borders, Bruce; Teskey, Robert] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30601 USA. [Maier, Chris] US Forest Serv, Southern Res Stn, Res Triangle Pk, NC 27709 USA. [Will, Rodney] Oklahoma State Univ, Stillwater, OK 74077 USA. RP Teskey, R (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM rteskey@uga.edu RI Will, Rodney/G-8111-2011 FU State of Georgia Traditional Industries; USDA Forest, Service Southern Research Station [SRS09CA11330137137019] FX We thank Wen long Lu for doing the Visual Basic programming in this project. Funding was provided by the State of Georgia Traditional Industries Program in Pulp and Paper and by a Cooperative Agreement with the USDA Forest, Service Southern Research Station, SRS09CA11330137137019. NR 80 TC 9 Z9 11 U1 1 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 501 EP 514 DI 10.1016/j.foreco.2012.09.031 PG 14 WC Forestry SC Forestry GA 099XP UT WOS:000315659500055 ER PT J AU Wear, LR Aust, WM Bolding, MC Strahm, BD Dolloff, CA AF Wear, Laura R. Aust, W. Michael Bolding, M. Chad Strahm, Brian D. Dolloff, C. Andrew TI Effectiveness of best management practices for sediment reduction at operational forest stream crossings SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest stream crossings; Water quality; Forest operations; Low impact forestry ID EASTERN UNITED-STATES; SURFACE-WATER QUALITY; SOIL-EROSION; VIRGINIA PIEDMONT; ECONOMIC COSTS; ROAD; IMPACT; DELIVERY; IMPLEMENTATION; PROTECTION AB Temporary skid trail stream crossings have repeatedly been identified as having considerable potential to introduce sediment to streams. Forestry Best Management Practices (BMPs) have proven to be effective for controlling erosion and subsequent sedimentation, yet few studies have quantified sedimentation associated with various levels of BMPs for skidder stream crossings. Three skid trail stream crossing BMP treatments were installed and replicated three times to quantify BMP efficacy for reducing sedimentation. BMP treatments were: (1) slash, (2) mulch and grass seed, and (3) mulch, grass seed, and silt fence. Water samples were collected daily both upstream and downstream from operational skidder stream crossings for one year following timber harvesting and BMP treatment installation. Samples were evaluated for total suspended solids (TSSs). Results indicate that both slash and mulch treatments effectively reduced TSS following harvesting. Slash could be the preferred method of stream crossing closure, due to lower cost, especially if application is incorporated into logging operations. However, if slash was being utilized for biomass and was not available, seed and mulch is a viable option for stream crossing closure. The mulch, seed, and silt fence treatment was the most expensive treatment and led to increased TSS, probably due to silt fence installation disturbances near the streams. Thus, silt fences should not be installed directly adjacent to streambanks, if other alternatives exist. (C) 2012 Elsevier B.V. All rights reserved. C1 [Wear, Laura R.; Aust, W. Michael; Bolding, M. Chad; Strahm, Brian D.] Virginia Tech, Dept Forest Resources & Environm Conservat 0324, Blacksburg, VA 24061 USA. [Dolloff, C. Andrew] Virginia Tech, USDA, Forest Serv,Dept Fish & Wildlife Conservat 0321, Coldwater Fisheries Unit, Blacksburg, VA 24061 USA. RP Aust, WM (reprint author), Virginia Tech, Dept Forest Resources & Environm Conservat 0324, 228 Cheatham Hall, Blacksburg, VA 24061 USA. EM lauraw6@vt.edu; waust@vt.edu; bolding@vt.edu; brian.strahm@vt.edu; adoll@vt.edu FU USDA Forest Service; Virginia Department of Forestry; Virginia Tech Forest Operations and Business Research Cooperative FX This project was partially funded by the USDA Forest Service, the Virginia Department of Forestry, and the Virginia Tech Forest Operations and Business Research Cooperative. Logistical support was provided by MWV Corporation. NR 93 TC 13 Z9 13 U1 9 U2 52 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2013 VL 289 BP 551 EP 561 DI 10.1016/j.foreco.2012.10.035 PG 11 WC Forestry SC Forestry GA 099XP UT WOS:000315659500060 ER PT J AU Fan, ZF Moser, WK Hansen, MH Nelson, MD AF Fan, Zhaofei Moser, W. Keith Hansen, Mark H. Nelson, Mark D. TI Regional Patterns of Major Nonnative Invasive Plants and Associated Factors in Upper Midwest Forests SO FOREST SCIENCE LA English DT Article DE nonnative invasive plant; Forest Inventory Analysis; kernel density smoothing; probability; classification and regression tree; bootstrap ID UNITED-STATES; NATIVE RANGE; INVASIBILITY; LANDSCAPE; ROADS; COMMUNITIES; HYPOTHESIS; RELEASE; SUCCESS; THREATS AB Nonnative invasive plants (IPs) are rapidly spreading into natural ecosystems (e.g., forests and grasslands). Potential threats of IP invasion into natural ecosystems include biodiversity loss, structural and environmental change, habitat degradation, and economic losses. The Upper Midwest of the United States encompasses the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, and Wisconsin, a region populated with 46 million people. Concerns of IP threats to the productive timberlands in the region have emerged with rapid expansion of urban areas and associated land cover changes caused by increasing human disturbances. Using the strategic inventory data from the 2005-2006 US Department of Agriculture Forest Service Forest Inventory and Analysis program and other data such as forestland cover and transportation coverage/layers, we modeled the regional patterns of IPs by using a combination of nonparametric techniques, including classification and regression tree analysis, kernel density smoothing, and bootstrapping. For the Midwest region, a probability map and historical records of human-related introduction of IPs of interest suggests that invasive shrubs, herbs, and grasses were initially introduced into the central (sparsely forested) areas and then spread north and south (densely forested areas), whereas invasive vines spread primarily from the south into other parts of the region. The probability of IPs in densely forested areas (0.1) was one-fifth of that in sparsely forested areas. Shrubs are the predominant IP threat and are distributed across the vast region with the exception of the northern part. Invasive grasses and herbs are most abundant in the central part of the region, and invasive vines are most common in the southern part. Percent forest cover and road proximity (distance to roads) as indicators of anthropogenic disturbances, were the most significant drivers of IP occurrence/abundance. Site factors, including forest productivity and stand biodiversity, were significantly correlated with the occurrence of vines. FOR. SCI. 59(1):38-49. C1 [Fan, Zhaofei] Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA. [Moser, W. Keith; Hansen, Mark H.; Nelson, Mark D.] US Forest Serv, USDA, No Res Stn, Morgantown, WV USA. RP Fan, ZF (reprint author), Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA. EM zfan@cfr.msstate.edu; wkmoser@fs.fed.us; mhhansen@fs.fed.us; mdnelson@fs.fed.us FU USDA Forest Service, Northern Research Station [208-JV-1124-2305-107] FX We thank Dr. David L. Evans, Professor, Department of Forestry, Mississippi State University, for reviewing this manuscript. This study was funded by the USDA Forest Service, Northern Research Station (project 208-JV-1124-2305-107). NR 64 TC 5 Z9 5 U1 0 U2 63 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD FEB PY 2013 VL 59 IS 1 BP 38 EP 49 DI 10.5849/forsci.10-100 PG 12 WC Forestry SC Forestry GA 097LW UT WOS:000315476600004 ER PT J AU Thompson, MP Calkin, DE Finney, MA Gebert, KM Hand, MS AF Thompson, Matthew P. Calkin, David E. Finney, Mark A. Gebert, Krista M. Hand, Michael S. TI A Risk-Based Approach to Wildland Fire Budgetary Planning SO FOREST SCIENCE LA English DT Article DE wildfire suppression; incentives; risk assessment; wildfire economics ID SUPPRESSION COST FORECASTS; DECISION-MAKING; WILDFIRE SUPPRESSION; FOREST SERVICE; UNITED-STATES; MANAGEMENT; EXPENDITURES; POLICY; SIMULATION; AREA AB The financial impact of wildfire management within the USDA Forest Service challenges the ability of the agency to meet societal demands and maintain forest health. The extent of this financial crisis has been attributed to historical and continuing fire management practices, changing climatic conditions, and increasing human development in fire-prone areas, as well as the lack of financial accountability of fire managers and misaligned incentive structures. In this article, we focus on incentives related to cost containment. We review the literature on the incentive structure facing wildfire managers and describe how the incentive structure does not sufficiently reward cost containment. We then cover a range of possible approaches to promote cost containment, culminating in a novel solution premised on the application of actuarial principles to wildfire budgetary planning that we believe most closely aligns with the Forest Service's transition to risk-based management paradigms and that most comprehensively incentivizes containment across the spectrum of wildfire management activities. We illustrate through a proof of concept case study how risk-based performance measures would be calculated and compare our results with historic suppression expenditures. Preliminary results suggest that our simulation model performs well in a relative sense to identify high- and low-cost forests, and we detail modeling improvements to refine estimates. We then illustrate potential extension to an actuarial system, which would further incentivize appropriate risk management and cost containment across the fire management continuum. We address the strengths and weaknesses of the proposed approaches, including potential roadblocks to implementation, and conclude by summarizing our major findings and offer recommendations for future agency direction. FOR. SCI. 59(1):63-77. C1 [Thompson, Matthew P.; Calkin, David E.] US Forest Serv, USDA, RMRS, Missoula, MT USA. [Finney, Mark A.; Gebert, Krista M.; Hand, Michael S.] US Forest Serv, USDA, Missoula, MT USA. RP Thompson, MP (reprint author), US Forest Serv, USDA, RMRS, Missoula, MT USA. EM mpthompson02@fs.fed.us; decalkin@fs.fed.us; mfinney@fs.fed.us; kgebert@fs.fed.us; mshand@fs.fed.us RI Calkin, David/F-4727-2014 NR 62 TC 10 Z9 12 U1 1 U2 35 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD FEB PY 2013 VL 59 IS 1 BP 63 EP 77 DI 10.5849/forsci.09-124 PG 15 WC Forestry SC Forestry GA 097LW UT WOS:000315476600006 ER PT J AU Loughran, G Libbey, JE Uddowla, S Scallan, MF Ryan, MD Fujinami, RS Rieder, E Atkins, JF AF Loughran, G. Libbey, J. E. Uddowla, S. Scallan, M. F. Ryan, M. D. Fujinami, R. S. Rieder, E. Atkins, J. F. TI Theiler's murine encephalomyelitis virus contrasts with encephalomyocarditis and foot-and-mouth disease viruses in its functional utilization of the StopGo non-standard translation mechanism SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID CLEAVAGE ACTIVITIES; 2A-LIKE SEQUENCES; IN-VITRO; POLYPROTEIN; SITE; REGION; APHTHOVIRUS; PRECURSORS; CODON; MICE AB The picornaviruses' genome consists of a positive-sense ssRNA. Like many picornaviruses, cardioviruses synthesize two distinct polyprotein precursors from adjacent but non-overlapping genome segments. Both the [L-1ABCD-2A] and the [2BC-3ABCD] polyproteins are proteolytically processed to yield mature capsid and non-structural proteins, respectively. An unusual translational event, known as 'StopGo' or 'Stop-Carry on', is responsible for the release of the [L-1ABCD-2A] polyprotein from the ribosome and synthesis of the N-terminal amino acid of the [2BC-3ABCD] polyprotein. A common feature of these viruses is the presence of a highly conserved signature sequence for StopGo: -D(V/I)ExNPG(down arrow) P-, where -D(V/I)ExNPG are the last 7 aa of 2A, and the last P- is the first amino acid of 2B. Here, we report that, in contrast to encephalomyocarditis virus and foot-and-mouth disease virus, a functional StopGo does not appear to be essential for Theiler's murine encephalomyelitis virus viability when tested in vitro and in vivo. C1 [Loughran, G.; Atkins, J. F.] Natl Univ Ireland Univ Coll Cork, Dept Biochem, Cork, Ireland. [Libbey, J. E.; Fujinami, R. S.] Univ Utah, Dept Pathol, Salt Lake City, UT 84132 USA. [Uddowla, S.; Rieder, E.] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Scallan, M. F.; Atkins, J. F.] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland. [Ryan, M. D.] Univ St Andrews, Ctr Biomol Sci, St Andrews, Fife, Scotland. [Atkins, J. F.] Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA. RP Atkins, JF (reprint author), Natl Univ Ireland Univ Coll Cork, Dept Biochem, Western Gateway Bldg, Cork, Ireland. EM J.atkins@ucc.ie OI Loughran, Gary/0000-0002-2683-5597 FU Science Foundation Ireland [08/IN.1/B1889]; NIH [R03 MH098688, 1R01NS065714]; CRIS, Agricultural Research Service (ARS), US Department of Agriculture [1940-32000-053-00D] FX We are extremely grateful for the assistance generously volunteered by Professor Ray Gesteland. We also wish to thank Maurice O'Donoghue for technical assistance. This work was supported by Science Foundation Ireland grant 08/IN.1/B1889 and NIH R03 MH098688 to J. F. A., NIH 1R01NS065714 to R. S. F. Work conducted on FMDV was supported by CRIS project no. 1940-32000-053-00D, Agricultural Research Service (ARS), US Department of Agriculture (Dr Elizabeth Rieder). NR 25 TC 1 Z9 2 U1 0 U2 0 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD FEB PY 2013 VL 94 BP 348 EP 353 DI 10.1099/vir.0.047571-0 PN 2 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 098JR UT WOS:000315546100013 PM 23100365 ER PT J AU Park, SH Butcher, BG Anderson, Z Pellegrini, N Bao, ZM D'Amico, K Filiatrault, MJ AF Park, So Hae Butcher, Bronwyn G. Anderson, Zoe Pellegrini, Nola Bao, Zhongmeng D'Amico, Katherine Filiatrault, Melanie J. TI Analysis of the small RNA P16/RgsA in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 SO MICROBIOLOGY-SGM LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; SMALL REGULATORY RNAS; SIGMA-FACTOR RPOS; ESCHERICHIA-COLI; STRESS TOLERANCE; ENVIRONMENTAL FITNESS; FLUORESCENS CHA0; VIBRIO-CHOLERAE; NONCODING RNAS; BACILLUS-SUBTILIS AB Bacteria contain small non-coding RNAs (ncRNAs) that are responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syringae pv. tomato DC3000, P16, was investigated. We determined that RpoS regulates the expression of P16. We found that deletion of P16 results in increased sensitivity to hydrogen peroxide compared to the wild-type strain, suggesting that P16 plays a role in the bacteria's susceptibility to oxidative stress. Additionally the P16 mutant displayed enhanced resistance to heat stress. Our findings provide new information on the regulation and role of this ncRNA in P. syringae. C1 [Park, So Hae; Butcher, Bronwyn G.; Anderson, Zoe; Bao, Zhongmeng; D'Amico, Katherine; Filiatrault, Melanie J.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Pellegrini, Nola; D'Amico, Katherine; Filiatrault, Melanie J.] ARS, Plant Microbe Interact Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. RP Filiatrault, MJ (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM melanie.filiatrault@ars.usda.gov NR 65 TC 4 Z9 4 U1 2 U2 16 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1350-0872 J9 MICROBIOL-SGM JI Microbiology-(UK) PD FEB PY 2013 VL 159 BP 296 EP 306 DI 10.1099/mic.0.063826-0 PN 2 PG 11 WC Microbiology SC Microbiology GA 103QL UT WOS:000315933900010 PM 23258266 ER PT J AU Larsson, SJ Lipka, AE Buckler, ES AF Larsson, Sara J. Lipka, Alexander E. Buckler, Edward S. TI Lessons from Dwarf8 on the Strengths and Weaknesses of Structured Association Mapping SO PLOS GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; SPATIAL GENETIC-STRUCTURE; MULTILOCUS GENOTYPE DATA; FLOWERING-TIME; POPULATION-STRUCTURE; MAIZE DOMESTICATION; ARABIDOPSIS-THALIANA; INBRED LINES; STRATIFICATION; ARCHITECTURE AB The strengths of association mapping lie in its resolution and allelic richness, but spurious associations arising from historical relationships and selection patterns need to be accounted for in statistical analyses. Here we reanalyze one of the first generation structured association mapping studies of the Dwarf8 (d8) locus with flowering time in maize using the full range of new mapping populations, statistical approaches, and haplotype maps. Because this trait was highly correlated with population structure, we found that basic structured association methods overestimate phenotypic effects in the region, while mixed model approaches perform substantially better. Combined with analysis of the maize nested association mapping population (a multi-family crossing design), it is concluded that most, if not all, of the QTL effects at the general location of the d8 locus are from rare extended haplotypes that include other linked QTLs and that d8 is unlikely to be involved in controlling flowering time in maize. Previous independent studies have shown evidence for selection at the d8 locus. Based on the evidence of population bottleneck, selection patterns, and haplotype structure observed in the region, we suggest that multiple traits may be strongly correlated with population structure and that selection on these traits has influenced segregation patterns in the region. Overall, this study provides insight into how modern association and linkage mapping, combined with haplotype analysis, can produce results that are more robust. C1 [Larsson, Sara J.; Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Lipka, Alexander E.; Buckler, Edward S.] Agr Res Serv, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Larsson, SJ (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. EM esb33@cornell.edu OI Buckler, Edward/0000-0002-3100-371X FU NSF Plant Genome Program [DBI-0321467, DBI-0820619, DBI-0638566]; USDA-ARS; Syngenta Fellowship FX This work was funded by NSF Plant Genome Program (DBI-0321467, DBI-0820619, DBI-0638566) and USDA-ARS. Graduate work of SJL was partially funded by a Syngenta Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 40 Z9 44 U1 4 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD FEB PY 2013 VL 9 IS 2 AR e1003246 DI 10.1371/journal.pgen.1003246 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 099RG UT WOS:000315638300010 PM 23437002 ER PT J AU Schardl, CL Young, CA Hesse, U Amyotte, SG Andreeva, K Calie, PJ Fleetwood, DJ Haws, DC Moore, N Oeser, B Panaccione, DG Schweri, KK Voisey, CR Farman, ML Jaromczyk, JW Roe, BA O'Sullivan, DM Scott, B Tudzynski, P An, ZQ Arnaoudova, EG Bullock, CT Charlton, ND Chen, L Cox, M Dinkins, RD Florea, S Glenn, AE Gordon, A Guldener, U Harris, DR Hollin, W Jaromczyk, J Johnson, RD Khan, AK Leistner, E Leuchtmann, A Li, CJ Liu, JG Liu, JZ Liu, M Mace, W Machado, C Nagabhyru, P Pan, J Schmid, J Sugawara, K Steiner, U Takach, JE Tanaka, E Webb, JS Wilson, EV Wiseman, JL Yoshida, R Zeng, Z AF Schardl, Christopher L. Young, Carolyn A. Hesse, Uljana Amyotte, Stefan G. Andreeva, Kalina Calie, Patrick J. Fleetwood, Damien J. Haws, David C. Moore, Neil Oeser, Birgitt Panaccione, Daniel G. Schweri, Kathryn K. Voisey, Christine R. Farman, Mark L. Jaromczyk, Jerzy W. Roe, Bruce A. O'Sullivan, Donal M. Scott, Barry Tudzynski, Paul An, Zhiqiang Arnaoudova, Elissaveta G. Bullock, Charles T. Charlton, Nikki D. Chen, Li Cox, Murray Dinkins, Randy D. Florea, Simona Glenn, Anthony E. Gordon, Anna Gueldener, Ulrich Harris, Daniel R. Hollin, Walter Jaromczyk, Jolanta Johnson, Richard D. Khan, Anar K. Leistner, Eckhard Leuchtmann, Adrian Li, Chunjie Liu, Jinge Liu, Jinze Liu, Miao Mace, Wade Machado, Caroline Nagabhyru, Padmaja Pan, Juan Schmid, Jan Sugawara, Koya Steiner, Ulrike Takach, Johanna E. Tanaka, Eiji Webb, Jennifer S. Wilson, Ella V. Wiseman, Jennifer L. Yoshida, Ruriko Zeng, Zheng TI Plant-Symbiotic Fungi as Chemical Engineers: Multi-Genome Analysis of the Clavicipitaceae Reveals Dynamics of Alkaloid Loci SO PLOS GENETICS LA English DT Article ID INDUCED POINT MUTATION; NONRIBOSOMAL PEPTIDE SYNTHETASE; PERENNIAL RYEGRASS; CLAVICEPS-PURPUREA; ERGOT ALKALOIDS; INDOLE-DITERPENE; GENE-CLUSTER; SEQUENCE ALIGNMENT; CYTOCHROME-P450 MONOOXYGENASE; NEOTYPHODIUM-UNCINATUM AB The fungal family Clavicipitaceae includes plant symbionts and parasites that produce several psychoactive and bioprotective alkaloids. The family includes grass symbionts in the epichloae clade (Epichloe and Neotyphodium species), which are extraordinarily diverse both in their host interactions and in their alkaloid profiles. Epichloae produce alkaloids of four distinct classes, all of which deter insects, and some-including the infamous ergot alkaloids-have potent effects on mammals. The exceptional chemotypic diversity of the epichloae may relate to their broad range of host interactions, whereby some are pathogenic and contagious, others are mutualistic and vertically transmitted (seed-borne), and still others vary in pathogenic or mutualistic behavior. We profiled the alkaloids and sequenced the genomes of 10 epichloae, three ergot fungi (Claviceps species), a morning-glory symbiont (Periglandula ipomoeae), and a bamboo pathogen (Aciculosporium take), and compared the gene clusters for four classes of alkaloids. Results indicated a strong tendency for alkaloid loci to have conserved cores that specify the skeleton structures and peripheral genes that determine chemical variations that are known to affect their pharmacological specificities. Generally, gene locations in cluster peripheries positioned them near to transposon-derived, AT-rich repeat blocks, which were probably involved in gene losses, duplications, and neofunctionalizations. The alkaloid loci in the epichloae had unusual structures riddled with large, complex, and dynamic repeat blocks. This feature was not reflective of overall differences in repeat contents in the genomes, nor was it characteristic of most other specialized metabolism loci. The organization and dynamics of alkaloid loci and abundant repeat blocks in the epichloae suggested that these fungi are under selection for alkaloid diversification. We suggest that such selection is related to the variable life histories of the epichloae, their protective roles as symbionts, and their associations with the highly speciose and ecologically diverse cool-season grasses. C1 [Schardl, Christopher L.; Hesse, Uljana; Amyotte, Stefan G.; Andreeva, Kalina; Schweri, Kathryn K.; Farman, Mark L.; Chen, Li; Florea, Simona; Hollin, Walter; Jaromczyk, Jolanta; Liu, Jinge; Liu, Miao; Machado, Caroline; Nagabhyru, Padmaja; Pan, Juan; Webb, Jennifer S.; Wilson, Ella V.; Wiseman, Jennifer L.] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Young, Carolyn A.; Charlton, Nikki D.; Takach, Johanna E.] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK USA. [Calie, Patrick J.] Eastern Kentucky Univ, Richmond, KY 40475 USA. [Fleetwood, Damien J.] Univ Auckland, AgRes Lab, Sch Biol Sci, Auckland 1, New Zealand. [Haws, David C.; Yoshida, Ruriko] Univ Kentucky, Dept Stat, Lexington, KY USA. [Moore, Neil; Jaromczyk, Jerzy W.; Arnaoudova, Elissaveta G.; Bullock, Charles T.; Harris, Daniel R.; Liu, Jinze; Zeng, Zheng] Univ Kentucky, Dept Comp Sci, Lexington, KY 40506 USA. [Oeser, Birgitt; Tudzynski, Paul] Univ Munster, Inst Plant Biol & Biotechnol, Munster, Germany. [Panaccione, Daniel G.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Voisey, Christine R.; Johnson, Richard D.; Mace, Wade] AgResearch, Grasslands Res Ctr, Palmerston North, New Zealand. [Roe, Bruce A.] Univ Oklahoma, Dept Chem & Biochem, Stephenson Res & Technol Ctr, Norman, OK 73019 USA. [Gordon, Anna] Natl Inst Agr Bot, John Bingham Lab, Cambridge, England. [Scott, Barry; Cox, Murray; Schmid, Jan] Massey Univ, Inst Mol BioSci, Palmerston North, New Zealand. [An, Zhiqiang] Univ Texas Hlth Sci Ctr, Texas Therapeut Inst, Houston, TX USA. [Dinkins, Randy D.] ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY USA. [Glenn, Anthony E.] ARS, Toxicol & Mycotoxin Res Unit, Richard B Russell Res Ctr, USDA, Athens, GA USA. [Gueldener, Ulrich] Helmholtz Zentrum Munchen GmbH, Inst Bioinformat & Syst Biol, Neuherberg, Germany. [Khan, Anar K.] Invermay Agr Ctr, Mosgiel, New Zealand. [Leistner, Eckhard; Steiner, Ulrike] Univ Bonn, Inst Fuer Pharmazeut Biol, Bonn, Germany. [Leuchtmann, Adrian] ETH, Inst Integrat Biol, Zurich, Switzerland. [Li, Chunjie] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730000, Peoples R China. [Sugawara, Koya] Natl Agr & Food Res Org NARO, Inst Livestock & Grassland Sci, Nasushiobara, Tochigi, Japan. [Sugawara, Koya] Ishikawa Prefectural Univ, Nonoichi, Ishikawa, Japan. RP Schardl, CL (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM Schardl@uky.edu RI Schmid, Jan/A-6863-2011; Scott, David/J-3135-2013; Nagabhyru, Padmaja/B-4263-2014; Guldener, Ulrich/G-5227-2012; O'Sullivan, Donal/C-8157-2014; Leuchtmann, Adrian/I-2922-2012; Schardl, Christopher/P-5655-2014; Cox, Murray/A-1459-2012; OI Dinkins, Randy/0000-0002-2127-273X; Schmid, Jan/0000-0001-8794-4175; Guldener, Ulrich/0000-0001-5052-8610; O'Sullivan, Donal/0000-0003-4889-056X; Schardl, Christopher/0000-0003-2197-0842; Cox, Murray/0000-0003-1936-0236; Young, Carolyn/0000-0003-0406-8398 FU National Science Foundation [EF-0523661, EPS-0814194]; U.S. Department of Agriculture [2005-35319-16141, 2008-35318-04549, 2010-34457-21269]; National Institutes of Health [R01GM086888, 2 P20 RR-16481]; Samuel Roberts Noble Foundation; Arnold and Mabel Beckman Foundation; UK Biotechnology and Biological Sciences Research Council [BB/G020418/1]; Deutsche Forschungsgemeinschaft [Tu50/17-1]; NSF [EPS-0447479] FX This study was supported in the United States by National Science Foundation grants EF-0523661 and EPS-0814194; U.S. Department of Agriculture grants 2005-35319-16141, 2008-35318-04549, and 2010-34457-21269; National Institutes of Health grants R01GM086888 and 2 P20 RR-16481; the Samuel Roberts Noble Foundation; and the Arnold and Mabel Beckman Foundation's Beckman Scholars Program (to Kathryn K Schweri). This study was supported in Europe by UK Biotechnology and Biological Sciences Research Council grant number BB/G020418/1 (to Donal M O'Sullivan), and Deutsche Forschungsgemeinschaft grant number Tu50/17-1 (to Paul Tudzynski). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Richard M. Higashi and Teresa W.M. Fan of the University of Louisville Center for Regulatory and Environmental Analytical Metabolomics (supported by NSF EPSCoR grant EPS-0447479), together with Jerome R. Faulkner, University of Kentucky, and for identification of 1-acetamidopyrrolizidine; Abbe Kesterson and Alfred D. Byrd of the University of Kentucky Advanced Genetic Technologies Center for assistance in DNA sequencing; and John May of the University of Kentucky Environmental Research Training Laboratories for assistance in loline alkaloid analysis. This is publication number 13-12-004 of the Kentucky Agricultural Experiment Station, published with approval of the director. NR 121 TC 107 Z9 115 U1 8 U2 107 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD FEB PY 2013 VL 9 IS 2 AR e1003323 DI 10.1371/journal.pgen.1003323 PG 26 WC Genetics & Heredity SC Genetics & Heredity GA 099RG UT WOS:000315638300066 PM 23468653 ER PT J AU Liu, B Behura, SK Clem, RJ Schneemann, A Becnel, J Severson, DW Zhou, L AF Liu, Bo Behura, Susanta K. Clem, Rollie J. Schneemann, Anette Becnel, James Severson, David W. Zhou, Lei TI P53-Mediated Rapid Induction of Apoptosis Conveys Resistance to Viral Infection in Drosophila melanogaster SO PLOS PATHOGENS LA English DT Article ID FLOCK-HOUSE-VIRUS; MOSQUITO AEDES-AEGYPTI; BACULOVIRUS INFECTION; ANTIVIRAL RESPONSE; DENGUE-2 VIRUS; DNA-DAMAGE; CELLS; PROTEIN; GENE; REPLICATION AB Arthropod-borne pathogens account for millions of deaths each year. Understanding the genetic mechanisms controlling vector susceptibility to pathogens has profound implications for developing novel strategies for controlling insect-transmitted infectious diseases. The fact that many viruses carry genes that have anti-apoptotic activity has long led to the hypothesis that induction of apoptosis could be a fundamental innate immune response. However, the cellular mechanisms mediating the induction of apoptosis following viral infection remained enigmatic, which has prevented experimental verification of the functional significance of apoptosis in limiting viral infection in insects. In addition, studies with cultured insect cells have shown that there is sometimes a lack of apoptosis, or the pro-apoptotic response happens relatively late, thus casting doubt on the functional significance of apoptosis as an innate immunity. Using in vivo mosquito models and the native route of infection, we found that there is a rapid induction of reaper-like pro-apoptotic genes within a few hours following exposure to DNA or RNA viruses. Recapitulating a similar response in Drosophila, we found that this rapid induction of apoptosis requires the function of P53 and is mediated by a stress-responsive regulatory region upstream of reaper. More importantly, we showed that the rapid induction of apoptosis is responsible for preventing the expression of viral genes and blocking the infection. Genetic changes influencing this rapid induction of reaper-like pro-apoptotic genes led to significant differences in susceptibility to viral infection. C1 [Liu, Bo; Zhou, Lei] Univ Florida, Coll Med, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA. [Liu, Bo; Zhou, Lei] Univ Florida, Coll Med, UF Shands Canc Ctr, Gainesville, FL USA. [Behura, Susanta K.; Severson, David W.] Univ Notre Dame, Dept Biol Sci, Eck Inst Global Hlth, Notre Dame, IN 46556 USA. [Clem, Rollie J.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Schneemann, Anette] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA. [Becnel, James] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Liu, B (reprint author), Univ Florida, Coll Med, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA. EM leizhou@ufl.edu RI Liu, Bo/K-1268-2016; OI BEHURA, SUSANTA/0000-0002-0654-6288 FU NIH [R21-AI070513, R56-AI079074, GM053491]; UF Medical Guild [00088454]; NIH/NIAID [R01-AI059342]; University of Florida FX This work was supported in part by NIH R21-AI070513 and R56-AI079074 to LZ, UF Medical Guild 00088454 to BL, NIH/NIAID R01-AI059342 to DWS and NIH GM053491 to AS. Publication of this article was funded in part by the University of Florida Open-Access Publishing Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 22 Z9 23 U1 3 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD FEB PY 2013 VL 9 IS 2 AR e1003137 DI 10.1371/journal.ppat.1003137 PG 12 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA 099UU UT WOS:000315648900004 PM 23408884 ER PT J AU Chattanathan, SA Clement, TP Kanel, SR Barnett, MO Chatakondi, N AF Chattanathan, S. A. Clement, T. P. Kanel, S. R. Barnett, M. O. Chatakondi, N. TI Remediation of Uranium-contaminated Groundwater by Sorption onto Hydroxyapatite Derived from Catfish Bones SO WATER AIR AND SOIL POLLUTION LA English DT Article DE U(VI); Hydroxyapatite; Permeable reactive barrier; Groundwater remediation ID REMOVAL; ADSORPTION; APATITE; STABILITY; METALS; WASTE; OXIDE; PH AB Hydroxyapatite (HA) was prepared from catfish bones, identified as catfish HA (CFHA), using mechanical and chemical treatment methods. CFHA was characterized by x-ray diffraction (XRD) and scanning electron microscope (SEM) techniques to confirm the presence of HA. The ability of CFHA to remove uranium (U(VI)) from aqueous phase was investigated using both batch and column experiments. Adsorption experiments in batch experiments were carried by varying pH, preparation temperature, and particle size. The data shows that the maximum adsorption occurred between pH5.5 and 7. The adsorption of U(VI) on CFHA was greater at 300 degrees C than at 100 degrees C. Batch data shows that the smallest particles, with maximum surface area, exhibited significant U (VI) removal efficiency. Column experiments were conducted using the smallest CFHA particles at different flow rates and breakthrough profiles were obtained. The scalability of the U(VI) removal process was tested by comparing the performances of columns packed with different CFHA. The results indicated that the reaction scales to the mass concentration of the reactants (CFHA and U(VI)). We also found that at pH7, the CFHA packed in the column has the potential to remove about 3.9 mg of U(VI) per gram. Our study shows that CFHA may be used in permeable reactive barriers for remediating U(VI)-contaminated groundwater plumes. C1 [Chattanathan, S. A.; Clement, T. P.; Kanel, S. R.; Barnett, M. O.] Auburn Univ, Dept Civil & Environm Engn, Auburn, AL 36849 USA. [Kanel, S. R.] USAF, Inst Technol, Wright Patterson AFB, OH 45433 USA. [Chatakondi, N.] Mississippi State Univ, USDA ARS, Catfish Genet Res Unit, Mississippi State, MS 39762 USA. RP Kanel, SR (reprint author), USAF, Inst Technol, Wright Patterson AFB, OH 45433 USA. EM sushil.kanel.ctr@afit.edu FU US Department of Energy at Auburn University [DE-FG02-06ER64213] FX This work was supported by the US Department of Energy Grant No. DE-FG02-06ER64213 at Auburn University. NR 28 TC 5 Z9 5 U1 2 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD FEB PY 2013 VL 224 IS 2 AR 1429 DI 10.1007/s11270-012-1429-5 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA 094RK UT WOS:000315281300033 ER PT J AU Brodersen, CR Choat, B Chatelet, DS Shackel, KA Matthews, MA McElrone, AJ AF Brodersen, Craig R. Choat, Brendan Chatelet, David S. Shackel, Kenneth A. Matthews, Mark A. McElrone, Andrew J. TI XYLEM VESSEL RELAYS CONTRIBUTE TO RADIAL CONNECTIVITY IN GRAPEVINE STEMS (VITIS VINIFERA AND V. ARIZONICA; VITACEAE) SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE embolism; grapevine; high-resolution computed tomography; HRCT; hydraulic transport; microtomography; vascular pathogens; vessels; water; xylem ID PIERCES-DISEASE RESISTANCE; INTERVESSEL PIT MEMBRANES; FASTIDIOSA; DIFFERENTIATION; ANATOMY; PLANTS; VULNERABILITY; POPULATION; CAVITATION; PATTERNS AB Premise of the study: Xylem network connections play an important role in water and nutrient transport in plants, but also facilitate the spread of air embolisms and xylem-dwelling pathogens. This study describes the structure and function of vessel relays found in grapevine xylem that form radial and tangential connections between spatially discrete vessels. Methods: We used high-resolution computed tomography, light microscopy, scanning electron microscopy, and single-vessel dye injections to characterize vessel relays in stems and compare their distributions and structure in two Vitis species. Key results: Vessel relays were composed of 1-8 narrow diameter (similar to 25 mu m) vessel elements and were oriented radially, connecting vessels via scalariform pitting within a xylem sector delineated by rays. The functional connectedness of vessels linked by vessel relays was confirmed with single-vessel dye injections. In 4.5-cm sections of stem tissue, there were 26% more vessel relays in V. vinifera compared with V. arizonica. Conclusions: Because of their spatial distribution within Vitis xylem, vessel relays increase the connectivity between vessels that would otherwise remain isolated. Differences in vessel relays between Vitis species suggest these anatomical features could contribute to disease and embolism resistance in some species. C1 [Brodersen, Craig R.] Univ Florida, Ctr Citrus Res & Educ, Dept Hort Sci, Lake Alfred, FL 33850 USA. [Choat, Brendan] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. [Chatelet, David S.] Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. [Shackel, Kenneth A.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Matthews, Mark A.; McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [McElrone, Andrew J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM ajmcelrone@ucdavis.edu RI Matthews, Mark/A-5714-2010; Brodersen, Craig/J-1112-2016 OI Brodersen, Craig/0000-0002-0924-2570 FU National Science Foundation [0818479]; USDA-ARS CRIS [5306-21220-004-00]; Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy [DE-AC02-05CH11231] FX The authors thank A. MacDowell and D. Parkinson of beamline 8.3.2 at the Lawrence Berkeley National Laboratory Advanced Light Source for technical assistance, A. Walker for providing grapevine material, and L. Jordan, J. Shogren, and K. Zhao for data collection and analysis. They also thank S. Jansen for helpful comments and S. Carlquist for a careful critique that significantly improved the clarity of the manuscript. This work was supported by a National Science Foundation grant (0818479) and USDA-ARS CRIS funding (Research Project 5306-21220-004-00). The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under contract no. DE-AC02-05CH11231. NR 42 TC 21 Z9 22 U1 2 U2 54 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD FEB PY 2013 VL 100 IS 2 BP 314 EP 321 DI 10.3732/ajb.1100606 PG 8 WC Plant Sciences SC Plant Sciences GA 098TG UT WOS:000315571400004 PM 23345417 ER PT J AU Barnard, DM Lachenbruch, B McCulloh, KA Kitin, P Meinzer, FC AF Barnard, David M. Lachenbruch, Barbara McCulloh, Katherine A. Kitin, Peter Meinzer, Frederick C. TI DO RAY CELLS PROVIDE A PATHWAY FOR RADIAL WATER MOVEMENT IN THE STEMS OF CONIFER TREES? SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE conifers; drought; hydraulic architecture; hydraulic conductivity; radial conductivity; ray parenchyma; ray tracheids; xylem anatomy ID HYDRAULIC RESISTANCE; WOODY-PLANTS; PINE TREES; XYLEM; TRANSPORT; STORAGE; TISSUE; VULNERABILITY; CONDUCTIVITY; CAVITATION AB Premise of the study: The pathway of radial water movement in tree stems presents an unknown with respect to whole-tree hydraulics. Radial profiles have shown substantial axial sap flow in deeper layers of sapwood (that may lack direct connection to transpiring leaves), which suggests the existence of a radial pathway for water movement. Rays in tree stems include ray tracheids and/or ray parenchyma cells and may offer such a pathway for radial water transport. This study investigated relationships between radial hydraulic conductivity (k(s-rad)) and ray anatomical and stem morphological characteristics in the stems of three conifer species whose distributions span a natural aridity gradient across the Cascade Mountain range in Oregon, United States. Methods: The k(s-rad) was measured with a high-pressure flow meter. Ray tracheid and ray parenchyma characteristics and water transport properties were visualized using autofluorescence or confocal microscopy. Key results: The k(s-rad) did not vary predictably with sapwood depth among species and populations. Dye tracer did not infiltrate ray tracheids, and infiltration into ray parenchyma was limited. Regression analyses revealed inconsistent relationships between k(s-rad) and selected anatomical or growth characteristics when ecotypes were analyzed individually and weak relationships between k(s-rad) and these characteristics when data were pooled by tree species. Conclusions: The lack of significant relationships between k(s-rad) and the ray and stem morphologies we studied, combined with the absence of dye tracer in ray tracheid and limited movement of dye into ray parenchyma suggests that rays may not facilitate radial water transport in the three conifer species studied. C1 [Barnard, David M.; Lachenbruch, Barbara; McCulloh, Katherine A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Kitin, Peter] USDA, Inst Microbial & Biochem Technol, Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Meinzer, Frederick C.] USDA, Forest Serv, Forestry Sci Lab, Corvallis, OR 97331 USA. RP Barnard, DM (reprint author), Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. EM dave.barnard@colostate.edu RI Meinzer, Frederick/C-3496-2012; Kitin, Peter/D-9244-2014 FU U.S. Department of Agriculture Wood Utilization Research Special Grant; National Science Foundation [09-19871]; Joint Venture Agreement [07-JV-468]; U.S. Department of Agriculture, Forest Service Pacific Northwest Research Station FX The authors thank Cascade Timber Consulting for supply of study material; P. Denne for discussion, B. Barnard for assistance with data collection, and D. Barnard for helpful comments. This work was supported in part by the U.S. Department of Agriculture Wood Utilization Research Special Grant to Oregon State University, by National Science Foundation (09-19871) and Joint Venture Agreement (07-JV-468) with the U.S. Department of Agriculture, Forest Service Pacific Northwest Research Station. NR 48 TC 9 Z9 9 U1 2 U2 52 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD FEB PY 2013 VL 100 IS 2 BP 322 EP 331 DI 10.3732/ajb.1200333 PG 10 WC Plant Sciences SC Plant Sciences GA 098TG UT WOS:000315571400005 PM 23347974 ER PT J AU Johnson, DM Domec, JC Woodruff, DR McCulloh, KA Meinzer, FC AF Johnson, Daniel M. Domec, Jean-Christophe Woodruff, David R. McCulloh, Katherine A. Meinzer, Frederick C. TI CONTRASTING HYDRAULIC STRATEGIES IN TWO TROPICAL LIANAS AND THEIR HOST TREES SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE Anacardium excelsum; drought stress; embolism; gas exchange; Prionostemma aspera; transpiration; Trichostigma octandrum; water relations ID BELOW-GROUND COMPETITION; BARRO-COLORADO ISLAND; FOREST CANOPY TREES; WATER TRANSPORT; STOMATAL CONTROL; SAFETY MARGINS; WOODY-PLANTS; XYLEM VULNERABILITY; EMBOLISM FORMATION; NEGATIVE-PRESSURE AB Premise of the Study: Tropical liana abundance has been increasing over the past 40 yr, which has been associated with reduced rainfall. The proposed mechanism allowing lianas to thrive in dry conditions is deeper root systems than co-occurring trees, although we know very little about the fundamental hydraulic physiology of lianas. Methods: To test the hypothesis that two abundant liana species would physiologically outperform their host tree under reduced water availability, we measured rooting depth, hydraulic properties, plant water status, and leaf gas exchange during the dry season in a seasonally dry tropical forest. We also used a model to compare water use by one of the liana species and the host tree during drought. Key Results: All species measured were shallowly rooted. The liana species were more vulnerable to embolism than host trees and experienced water potentials that were predicted to result in substantial hydraulic losses in both leaves and stems. Water potentials measured in host trees were not negative enough to result in significant hydraulic losses. Model results predicted the liana to have greater gas exchange than its host tree during drought and nondrought conditions. Conclusions: The host tree species had a more conservative strategy for maintenance of the soil-to-leaf hydraulic pathway than the lianas it supported. The two liana species experienced embolism in stems and leaves, based on vulnerability curves and water potentials. These emboli were presumably repaired before the next morning. However, in the host tree species, reduced stomatal conductance prevented leaf or stem embolism. C1 [Johnson, Daniel M.; Domec, Jean-Christophe] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Domec, Jean-Christophe] Bordeaux Sci AGRO, UMR TCEM INRA 1220, F-33175 Gradignan, France. [Woodruff, David R.; Meinzer, Frederick C.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [McCulloh, Katherine A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. RP Johnson, DM (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM dan.johnson@duke.edu RI Johnson, Daniel/E-6789-2011; Meinzer, Frederick/C-3496-2012 OI Johnson, Daniel/0000-0001-5890-3147; FU National Science Foundation [IOB-0919871] FX This research was supported by National Science Foundation grant IOB-0919871. The authors thank S. Schnitzer and W. Carson for discussions that benefited this study. We also thank A. Roddy for daily critical discussions of ideas and for sharing his equipment and technical knowledge. NR 74 TC 12 Z9 12 U1 6 U2 70 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD FEB PY 2013 VL 100 IS 2 BP 374 EP 383 DI 10.3732/ajb.1200590 PG 10 WC Plant Sciences SC Plant Sciences GA 098TG UT WOS:000315571400011 PM 23328691 ER PT J AU Franzluebbers, AJ AF Franzluebbers, Alan J. TI Pursuing robust agroecosystem functioning through effective soil organic carbon management SO CARBON MANAGEMENT LA English DT Article ID SUSTAINABLE LAND MANAGEMENT; NITROUS-OXIDE EMISSIONS; SOUTHERN PIEDMONT USA; SHORT-TERM CHANGES; POULTRY LITTER; WATER-QUALITY; CONSERVATION TILLAGE; NO-TILL; STRATIFICATION RATIO; PHYSICAL-PROPERTIES AB Soil organic matter is a key indicator of many ecosystem functions, particularly in agricultural systems. With carbon as its majority constituent (similar to 58%), soil organic matter is a key variable relating production and environmental responses. However, it is argued that depth distribution of soil organic carbon (SOC) may be more important in understanding how agriculture affects ecosystem services derived from soil than the total quantity of SOC. Conservation agricultural systems lead to highly stratified SOC, which helps to protect soil from erosion and prevent runoff loss of nutrients (i.e., water quality improvement), creates a concentrated organic habitat for nutrient storage and soil biological diversity (i.e., soil quality improvement) and promotes a structurally stable pore network connecting surface and subsurface to avoid negative impacts on soil aeration and GHG emissions (i.e., air quality improvement). A protocol is described to calculate the stratification ratio of soil organic matter fractions from a diversity of sampling procedures, which may be relevant in different ecoregions and conditions of the soil. C1 N Carolina State Univ, USDA, ARS, Dept Soil Sci, Raleigh, NC 27695 USA. RP Franzluebbers, AJ (reprint author), N Carolina State Univ, USDA, ARS, Dept Soil Sci, Campus Box 7619, Raleigh, NC 27695 USA. EM alan.franzluebbers@ars.usda.gov FU Agricultural Research Service USA FX Appreciation is extended to S Knapp for technical assistance in previous research and to the Agricultural Research Service USA scientists involved with and supporting the cross-location research project of GRACEnet (Greenhouse Gas Reduction through Agricultural Carbon Enhancement network). NR 99 TC 3 Z9 3 U1 4 U2 42 PU FUTURE SCI LTD PI LONDON PA UNITED HOUSE, 2 ALBERT PL, LONDON, N3 1QB, ENGLAND SN 1758-3004 EI 1758-3012 J9 CARBON MANAG JI Carbon Manag. PD FEB PY 2013 VL 4 IS 1 BP 43 EP 56 DI 10.4155/CMT.12.78 PG 14 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA 094BS UT WOS:000315238600013 ER PT J AU Hill, DE Dubey, JP AF Hill, D. E. Dubey, J. P. TI Toxoplasma gondii prevalence in farm animals in the United States SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Review DE Toxoplasma gondii; Food safety; Pigs; Lambs; Goats; Chickens ID HUMAN CONGENITAL TOXOPLASMOSIS; CHICKENS GALLUS-DOMESTICUS; RISK-FACTORS; TISSUE CYSTS; GENETIC-CHARACTERIZATION; HUMAN CONSUMPTION; DAIRY GOATS; SWINE FARMS; SEA OTTERS; INFECTION AB Toxoplasmosis, caused by Toxoplasma gondii, is one of the most common parasitic infections of humans and other warmblooded animals. It has been found worldwide and nearly one-third of humans have been exposed to the parasite. Congenital infection occurs when a woman becomes infected during pregnancy and transmits the parasite to the foetus. Besides congenital infection, humans become infected by ingesting food or water contaminated with sporulated oocysts from infected cat faeces or through ingestion of tissue cysts in undercooked or uncooked meat. Food animals (pigs, chickens, lambs and goats) become infected by the same routes, resulting in meat products containing tissue cysts, which can then infect consumers. Toxoplasma infection is common in food animals in the United States. Implementation of management factors such as biosecure confinement housing are important in reducing the levels of infection in animals destined for human consumption. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. C1 [Hill, D. E.; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC E, Beltsville, MD 20705 USA. RP Hill, DE (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC E, Beltsville, MD 20705 USA. EM dolores.hill@ars.usda.gov NR 86 TC 48 Z9 48 U1 1 U2 55 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 J9 INT J PARASITOL JI Int. J. Parasit. PD FEB PY 2013 VL 43 IS 2 SI SI BP 107 EP 113 DI 10.1016/j.ijpara.2012.09.012 PG 7 WC Parasitology SC Parasitology GA 095ZB UT WOS:000315372400002 PM 23201235 ER PT J AU Schares, G Langenmayer, MC Scharr, JC Minke, L Maksimov, P Maksimov, A Schares, S Barwald, A Basso, W Dubey, JP Conraths, FJ Gollnick, NS AF Schares, G. Langenmayer, M. C. Scharr, J. C. Minke, L. Maksimov, P. Maksimov, A. Schares, S. Baerwald, A. Basso, W. Dubey, J. P. Conraths, F. J. Gollnick, N. S. TI Novel tools for the diagnosis and differentiation of acute and chronic bovine besnoitiosis SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Besnoitia besnoiti; Surface antigen; Tachyzoite; Bradyzoite; Immunoblot; IFAT; ELISA; Avidity ID TOXOPLASMA-GONDII INFECTION; NEOSPORA-CANINUM INFECTION; LINKED-IMMUNOSORBENT-ASSAY; IGG AVIDITY ELISA; DAIRY-CATTLE; BRADYZOITES; TACHYZOITES; ANTIBODIES; ANTIGEN; IMMUNOFLUORESCENCE AB Diagnosis of acute bovine besnoitiosis is a major diagnostic problem. We developed diagnostic tests to serologically diagnose and differentiate acute and chronic cases of bovine besnoitiosis using affinity purified antigens of Besnoitia besnoiti tachyzoites in immunoblots and in both, a conventional ELISA and an avidity ELISA. Sera of acutely and chronically infected cattle were investigated using these tests. Acutely infected cattle initially recognised an antigen of 74 kDa relative molecular mass, followed by reactions with increasing intensity against 81 and 28 kDa antigens. In addition, faint reactions against antigens with 36, 37, 39 and 42 kDa molecular mass started soon after seroconversion and increased over time. An antigen of 45 kDa molecular mass was transiently recognised early after infection but not or only weakly in the chronic stage. At least two antigens, the 39 and the 42 kDa antigens, seem to be located on the surface of B. besnoiti tachyzoites as determined by biotinylation. Affinity purified antigen was used to establish an APure-BbELISA which showed excellent sensitivity (100%) relative to a serological reference system in naturally, most likely chronically, infected cattle. Specificity was also high (99.8%) as determined in cattle from herds with Neospora caninum-associated abortions. The antibody levels in APure-BbELISA were correlated with the parasite load in the skin or the mucous membrane of the vestibulum vaginae as determined by real-time PCR. In acute cases of bovine besnoitiosis (confirmed by the detection of low avidity IgG in the APure-BbELISA) first specific antibodies were detected by ELISA in all animals except one, at the same time or earlier than in the serological reference system. The detection of parasite DNA in skin by real-time PCR was clearly superior to serological analysis in detecting infected cattle during acute besnoitiosis. (C) 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. C1 [Schares, G.; Minke, L.; Maksimov, P.; Maksimov, A.; Schares, S.; Baerwald, A.; Conraths, F. J.] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, Wusterhausen, Germany. [Langenmayer, M. C.] Univ Munich, Fac Vet, Ctr Clin Vet Med, Inst Vet Pathol, Oberschleissheim, Germany. [Scharr, J. C.; Gollnick, N. S.] Univ Munich, Fac Vet, Ctr Clin Vet Med, Clin Ruminants Ambulatory & Herd Hlth Serv, Oberschleissheim, Germany. [Basso, W.] Univ Zurich, Inst Parasitol, CH-8057 Zurich, Switzerland. [Dubey, J. P.] ARS, USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville, MD USA. RP Schares, G (reprint author), Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, Wusterhausen, Germany. EM gereon.schares@fli.bund.de OI Schares, Gereon/0000-0002-3217-289X; Conraths, Franz/0000-0002-7400-9409 NR 47 TC 20 Z9 20 U1 0 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 J9 INT J PARASITOL JI Int. J. Parasit. PD FEB PY 2013 VL 43 IS 2 SI SI BP 143 EP 154 DI 10.1016/j.ijpara.2012.10.011 PG 12 WC Parasitology SC Parasitology GA 095ZB UT WOS:000315372400006 PM 23160022 ER PT J AU Kim, JH Campbell, BC Chan, KL Mahoney, N Haff, RP AF Kim, Jong H. Campbell, Bruce C. Chan, Kathleen L. Mahoney, Noreen Haff, Ronald P. TI Synergism of Antifungal Activity between Mitochondrial Respiration Inhibitors and Kojic Acid SO MOLECULES LA English DT Article DE kojic acid; Aspergillus; Penicillium; Acremonium; Scedosporium; yeast; hydrogen peroxide; mitochondrial respiration inhibitors; chemosensitization ID CANDIDA-ALBICANS; SACCHAROMYCES-CEREVISIAE; OXIDATIVE STRESS; ALTERNATIVE OXIDASE; CHEMOSENSITIZATION; ENHANCEMENT; ATOVAQUONE; RESISTANT; COSMETICS; GROWTH AB Co-application of certain types of compounds to conventional antimicrobial drugs can enhance the efficacy of the drugs through a process termed chemosensitization. We show that kojic acid (KA), a natural pyrone, is a potent chemosensitizing agent of complex III inhibitors disrupting the mitochondrial respiratory chain in fungi. Addition of KA greatly lowered the minimum inhibitory concentrations of complex III inhibitors tested against certain filamentous fungi. Efficacy of KA synergism in decreasing order was pyraclostrobin > kresoxim-methyl > antimycin A. KA was also found to be a chemosensitizer of cells to hydrogen peroxide (H2O2), tested as a mimic of reactive oxygen species involved in host defense during infection, against several human fungal pathogens and Penicillium strains infecting crops. In comparison, KA-mediated chemosensitization to complex III inhibitors/H2O2 was undetectable in other types of fungi, including Aspergillus flavus, A. parasiticus, and P. griseofulvum, among others. Of note, KA was found to function as an antioxidant, but not as an antifungal chemosensitizer in yeasts. In summary, KA could serve as an antifungal chemosensitizer to complex III inhibitors or H2O2 against selected human pathogens or Penicillium species. KA-mediated chemosensitization to H2O2 seemed specific for filamentous fungi. Thus, results indicate strain- and/or drug-specificity exist during KA chemosensitization. C1 [Kim, Jong H.; Campbell, Bruce C.; Chan, Kathleen L.; Mahoney, Noreen; Haff, Ronald P.] ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Kim, JH (reprint author), ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM jongheon.kim@ars.usda.gov; bruce.campbell@ars.usda.gov; kathy.chan@ars.usda.gov; noreen.mahoney@ars.usda.gov; ron.haff@ars.usda.gov FU USDA-ARS CRIS Project [5325-42000-037-00D] FX We thank Arun Balajee, Centers for Disease Control and Prevention, Atlanta, GA, USA, and David Stevens, Santa Clara Valley Medical Center, San Jose, CA, USA, for providing us the strains of A. terreus and A. fumigatus, respectively. We also thank Chang-Lin Xiao, Department of Plant Pathology, Washington State University, Wenatchee, WA, USA, for providing us the wild type and fludioxonil-resistant P. expansum strains. This research was conducted under USDA-ARS CRIS Project 5325-42000-037-00D. NR 40 TC 4 Z9 5 U1 1 U2 34 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD FEB PY 2013 VL 18 IS 2 BP 1564 EP 1581 DI 10.3390/molecules18021564 PG 18 WC Chemistry, Organic SC Chemistry GA 096JR UT WOS:000315400600017 PM 23353126 ER PT J AU Ali, A Tabanca, N Demirci, B Baser, KHC Ellis, J Gray, S Lackey, BR Murphy, C Khan, IA Wedge, DE AF Ali, Abbas Tabanca, Nurhayat Demirci, Betul Baser, K. Husnu Can Ellis, Jane Gray, Sandra Lackey, Brett R. Murphy, Christine Khan, Ikhlas A. Wedge, David E. TI Composition, Mosquito Larvicidal, Biting Deterrent and Antifungal Activity of Essential Oils of Different Plant Parts of Cupressus arizonica var. glabra ('Carolina Sapphire') SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Cupressus arizonica var. glabra; Carolina Sapphire; Antifungal; Mosquito; Larvicidal; Biting deterrent; Aedes aegypti ID ANTIMICROBIAL ACTIVITY; CHEMICAL-COMPOSITION; CONSTITUENTS; GREENE AB Essential oils obtained by hydrodistillation of female cones (FC), male cones (MC), needle-twigs (NT) and wood-bark (WB) of 'Carolina Sapphire' [Cupressus arizonica var glabra (Sudw.) Little] were analyzed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The main constituents of the investigated essential oils were as follows: FC: alpha-pinene 68.5% and myrcene 11.9%; MC: alpha-pinene 22.5%, epi-zonarene 9.9%, zonarene 6.9%, limonene 6.8% and cadina-3,5-diene 6.1%; NT: alpha-pinene 20.7%, epi-zonarene 10.4%, zonarene 7.6%, limonene 5.9% umbellulone 5.8% and cadina-3,5-diene 5.8%; WB: alpha-pinene 40.7%, limonene 3.2% and umbellulone 2.9%. Essential oils were evaluated for antifungal activity against the strawberry anthracnose causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Only WB essential oil showed moderate activity when compared with the commercial antifungal standards. Carolina Sapphire essential oils exhibited biting deterrent activity at 10 mu g/cm(2) with Biting Deterrence Index (BDI) values of 0.64, 0.59, 0.65 and 0.72, for FC, MC, NT and WB, respectively, and were significantly lower (P <= 0.05) than the synthetic insect repellent, DEET (N,N-diethyl-meta-toluamide), at 25 nmol/cm(2) against Aedes aegypti. The biting deterrence of NT and WB oils at 100 mu g/cm(2) with BDI values of 1.04 and 1.01, respectively, were similar, whereas the activity of MC (BDI=0.88) and PC (BDI=0.62) essential oils was lower than DEET at 25 nmol/cm(2). Essential oil from PC with LD50 of 33.7 ppm was most toxic against 1-day-old Ae. aegypti larvae at 24-h post treatment. Female cone essential oil was significantly more toxic than MC (LD50=53.6 ppm), NT (LD50=55.5 ppm) and WB (LD50=44.6 ppm) essential oils. C1 [Ali, Abbas; Tabanca, Nurhayat; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Demirci, Betul; Baser, K. Husnu Can] Anadolu Univ, Dept Pharmacognosy, Fac Pharm, TR-26470 Eskisehir, Turkey. [Ellis, Jane] Coll Charleston, Dept Biol, Charleston, SC 29424 USA. [Gray, Sandra; Lackey, Brett R.] Clemson Univ, Dept Anim & Vet Sci, Clemson, SC 29634 USA. [Murphy, Christine] Clemson Univ, Dept Entomol Soils & Plant Sci, Clemson, SC 29634 USA. [Wedge, David E.] Univ Mississippi, USDA ARS NPURU, University, MS 38677 USA. [Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. [Khan, Ikhlas A.] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia. [Baser, K. Husnu Can] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. RP Ali, A (reprint author), Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. EM aali@olemiss.edu RI Baser, K. Husnu Can/C-3109-2008; OI Baser, Kemal Husnu Can/0000-0003-2710-0231 FU USDA/ARS [56-6402-1-612]; Deployed War-Fighter Protection research program; US Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by USDA/ARS grant No. 56-6402-1-612 and Deployed War-Fighter Protection research program grant funded by the US Department of Defense through the Armed Forces Pest Management Board. We thank Ms. JL Robertson, Ms. R Pace and Ms. SR Weerasooriya for their help in bioassays and Dr James J. Becnel, Mosquito and Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, for supplying Ae. aegypti eggs. NR 24 TC 12 Z9 12 U1 0 U2 8 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD FEB PY 2013 VL 8 IS 2 BP 257 EP 260 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA 095XT UT WOS:000315369000032 PM 23513743 ER PT J AU Mirik, M Ansley, RJ Steddom, K Jones, DC Rush, CM Michels, GJ Elliott, NC AF Mirik, Mustafa Ansley, R. James Steddom, Karl Jones, David C. Rush, Charles M. Michels, Gerald J., Jr. Elliott, Norman C. TI Remote Distinction of A Noxious Weed (Musk Thistle: Carduus Nutans) Using Airborne Hyperspectral Imagery and the Support Vector Machine Classifier SO REMOTE SENSING LA English DT Article DE accuracy assessment; invasive plant; weed management; weed infestation; remote sensing; geospatial data ID MAPPING INVASIVE PLANTS; RHINOCYLLUS-CONICUS COLEOPTERA; MESQUITE PROSOPIS-GLANDULOSA; KNAPWEED CENTAUREA-MACULOSA; YELLOWSTONE-NATIONAL-PARK; HAWAIIAN RAIN-FORESTS; ACCURACY ASSESSMENT; BIOLOGICAL-CONTROL; LEAFY SPURGE; TRICHOSIROCALUS-HORRIDUS AB Remote detection of non-native invasive plant species using geospatial imagery may significantly improve monitoring, planning and management practices by eliminating shortfalls, such as observer bias and accessibility involved in ground-based surveys. The use of remote sensing for accurate mapping invasion extent and pattern offers several advantages, including repeatability, large area coverage, complete instead of sub-sampled assessments and greater cost-effectiveness over ground-based methods. It is critical for locating, early mapping and controlling small infestations before they reach economically prohibitive or ecologically significant levels over larger land areas. This study was designed to explore the ability of hyperspectral imagery for mapping infestation of musk thistle (Carduus nutans) on a native grassland during the preflowering stage in mid-April and during the peak flowering stage in mid-June using the support vector machine classifier and to assess and compare the resulting mapping accuracy for these two distinctive phenological stages. Accuracy assessment revealed that the overall accuracies were 79% and 91% for the classified images at preflowering and peak flowering stages, respectively. These results indicate that repeated detection of the infestation extent, as well as infestation severity or intensity, of this noxious weed in a spatial and temporal context is possible using hyperspectral remote sensing imagery. C1 [Mirik, Mustafa; Ansley, R. James] Texas A&M AgriLife Res & Extens Ctr, Vernon, TX 76385 USA. [Steddom, Karl] Texas A&M AgriLife Res & Extens Ctr, Overton, TX 75684 USA. [Jones, David C.] Ft Hood ITAM, DPTMS Range Div, Ft Hood, TX 76544 USA. [Rush, Charles M.; Michels, Gerald J., Jr.] Texas A&M AgriLife Res & Extens Ctr, Amarillo, TX 79012 USA. [Elliott, Norman C.] ARS, USDA, Plant Sci Res Lab, Stillwater, OK 74075 USA. RP Mirik, M (reprint author), Texas A&M AgriLife Res & Extens Ctr, 11708 Highway 70 S, Vernon, TX 76385 USA. EM mustafamirik@gmail.com; jansley@ag.tamu.edu; kcsteddom@ag.tamu.edu; dcjones@ag.tamu.edu; crush@ag.tamu.edu; gmichels@ag.tamu.edu; norman.elliott@ars.usda.gov FU USDA-ARS Areawide Pest Management Program [500-44-012-00] FX We are thankful to Johnny Bible, Robert Villarreal, Joy Newton, Sabina Mirik and Daniel Jiminez for their technical assistance. This study was partially funded by the USDA-ARS Areawide Pest Management Program. Project Number: 500-44-012-00. We also express our thanks to the three anonymous reviewers and editor who made critical suggestions and comments to improve the manuscript. NR 77 TC 16 Z9 17 U1 3 U2 46 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD FEB PY 2013 VL 5 IS 2 BP 612 EP 630 DI 10.3390/rs5020612 PG 19 WC Remote Sensing SC Remote Sensing GA 096KM UT WOS:000315402700008 ER PT J AU Filiatrault, MJ Stodghill, PV Wilson, J Butcher, BG Chen, HR Myers, CR Cartinhour, SW AF Filiatrault, Melanie J. Stodghill, Paul V. Wilson, Janet Butcher, Bronwyn G. Chen, Hanrong Myers, Christopher R. Cartinhour, Samuel W. TI CrcZ and CrcX regulate carbon source utilization in Pseudomonas syringae pathovar tomato strain DC3000 SO RNA BIOLOGY LA English DT Article DE psr; crcZ; crcY; crcX; Crc; ncRNA; RpoN; CbrA; CbrB; Pseudomonas syringae ID CATABOLITE REPRESSION CONTROL; SMALL RNAS; GLOBAL REGULATOR; SECONDARY METABOLISM; ESCHERICHIA-COLI; NONCODING RNAS; VIBRIO-HARVEYI; AERUGINOSA; PUTIDA; BACTERIA AB Small non-coding RNAs (ncRNAs) are important components of many regulatory pathways in bacteria and play key roles in regulating factors important for virulence. Carbon catabolite repression control is modulated by small RNAs (crcZ or crcZ and crcY) in Pseudomonas aeruginosa and Pseudomonas putida. In this study, we demonstrate that expression of crcZ and crcX (formerly designated psr1 and psr2, respectively) is dependent upon RpoN together with the two-component system CbrAB, and is influenced by the carbon source present in the medium in the model plant pathogen Pseudomonas syringae pv tomato DC3000. The distribution of the members of the Crc ncRNA family was also determined by screening available genomic sequences of the Pseudomonads. Interestingly, variable numbers of the Crc family members exist in Pseudomonas genomes. The ncRNAs are comprised of three main subfamilies, named CrcZ, CrcX and CrcY. Most importantly, the CrcX subfamily appears to be unique to all P. syringae strains sequenced to date. C1 [Filiatrault, Melanie J.; Stodghill, Paul V.; Wilson, Janet; Cartinhour, Samuel W.] ARS, Plant Microbe Interact Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY USA. [Filiatrault, Melanie J.; Butcher, Bronwyn G.; Cartinhour, Samuel W.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Myers, Christopher R.] Cornell Univ, Life Sci Core Labs Ctr, Ithaca, NY USA. [Chen, Hanrong; Myers, Christopher R.] Cornell Univ, Atom & Solid State Phys Lab, Ithaca, NY 14853 USA. RP Filiatrault, MJ (reprint author), ARS, Plant Microbe Interact Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY USA. EM melanie.filiatrault@ars.usda.gov NR 37 TC 11 Z9 13 U1 3 U2 14 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1547-6286 J9 RNA BIOL JI RNA Biol. PD FEB PY 2013 VL 10 IS 2 BP 245 EP 255 DI 10.4161/rna.23019 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 099AR UT WOS:000315591500010 PM 23353577 ER PT J AU Wilson, AD AF Wilson, Alphus D. TI Diverse Applications of Electronic-Nose Technologies in Agriculture and Forestry SO SENSORS LA English DT Review DE artificial olfaction; electronic aroma detection; volatile organic compounds ID QUARTZ-CRYSTAL MICROBALANCE; SEMICONDUCTOR GAS SENSORS; CONDUCTIVE POLYMER ANALYSIS; METAL-OXIDE NANOSTRUCTURES; ARTIFICIAL NEURAL-NETWORKS; VOLATILE ORGANIC-COMPOUNDS; COMPOSITE VAPOR DETECTORS; APRICOT PRUNUS-ARMENIACA; SALMON SALMO-SALAR; ISOLATES IN-VITRO AB Electronic-nose (e-nose) instruments, derived from numerous types of aroma-sensor technologies, have been developed for a diversity of applications in the broad fields of agriculture and forestry. Recent advances in e-nose technologies within the plant sciences, including improvements in gas-sensor designs, innovations in data analysis and pattern-recognition algorithms, and progress in material science and systems integration methods, have led to significant benefits to both industries. Electronic noses have been used in a variety of commercial agricultural-related industries, including the agricultural sectors of agronomy, biochemical processing, botany, cell culture, plant cultivar selections, environmental monitoring, horticulture, pesticide detection, plant physiology and pathology. Applications in forestry include uses in chemotaxonomy, log tracking, wood and paper processing, forest management, forest health protection, and waste management. These aroma-detection applications have improved plant-based product attributes, quality, uniformity, and consistency in ways that have increased the efficiency and effectiveness of production and manufacturing processes. This paper provides a comprehensive review and summary of a broad range of electronic-nose technologies and applications, developed specifically for the agriculture and forestry industries over the past thirty years, which have offered solutions that have greatly improved worldwide agricultural and agroforestry production systems. C1 US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res,Southern Hardwoods L, Stoneville, MS 38776 USA. RP Wilson, AD (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res,Southern Hardwoods L, POB 227, Stoneville, MS 38776 USA. EM dwilson02@fs.fed.us RI Wilson, Alphus/Q-2137-2015 OI Wilson, Alphus/0000-0003-2352-5232 NR 429 TC 56 Z9 58 U1 10 U2 198 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD FEB PY 2013 VL 13 IS 2 BP 2295 EP 2348 DI 10.3390/s130202295 PG 54 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA 096KS UT WOS:000315403300054 PM 23396191 ER PT J AU Runge, T Houtman, C Negri, A Heinricher, J AF Runge, Troy Houtman, Carl Negri, Alberto Heinricher, Jackie TI Timber bamboo pulp SO TAPPI JOURNAL LA English DT Article ID DELIGNIFICATION AB Fast-growing biomass, such as bamboo, has the potential to serve an important future role in the pulp and paper industry with potential to both lower resource costs and improve a product's sustainability. Moso bamboo is particularly interesting due to its fast growth and size, which allows it to be handled and chipped similarly to wood resources. In this study, we will share results of the chip preparation, kraft cooking, and ECF bleaching of this bamboo species and compare its pulpability, bleachability, and physical properties to a fast growing hybrid poplar tree. Results indicate that the bamboo chips cooked and bleached similarly to the poplar hardwood, allowing for co-cooking. The resulting pulps had superior tensile properties at low refining, but did have higher fines that lowered drainability as measured by Canadian Standard Freeness. The bamboo fiber morphology was also measured, indicating the fiber to have length weighted average fiber lengths and coarseness values to be greater than the poplar wood studied, which should allow this material to be used in many paper grades. C1 [Runge, Troy] Univ Wisconsin Madison, Madison, WI 53706 USA. [Houtman, Carl] USDA, Forest Prod Lab, Madison, WI USA. [Negri, Alberto] Dart Container Corp, Mason, MI USA. [Heinricher, Jackie] Booshoot Gardens LLC, Mt Vernon, IA USA. RP Runge, T (reprint author), Univ Wisconsin Madison, Madison, WI 53706 USA. EM trunge@wisc.edu NR 10 TC 1 Z9 1 U1 0 U2 14 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD FEB PY 2013 VL 12 IS 2 BP 9 EP 15 PG 7 WC Materials Science, Paper & Wood SC Materials Science GA 096MG UT WOS:000315407600002 ER PT J AU Blaustein, RA Pachepsky, Y Hill, RL Shelton, DR Whelan, G AF Blaustein, R. A. Pachepsky, Y. Hill, R. L. Shelton, D. R. Whelan, G. TI Escherichia coli survival in waters: Temperature dependence SO WATER RESEARCH LA English DT Article DE Database; Inactivation; Temperature effect; Water types; Q(10) model ID FECAL INDICATOR BACTERIA; LAKE WATER; ENTERIC BACTERIA; ESTUARINE WATERS; INACTIVATION; ENVIRONMENT; RIVER; PERSISTENCE; INTERFACE; DIFFUSION AB Knowing the survival rates of water-borne Escherichia coli is important in evaluating microbial contamination and making appropriate management decisions. E. coli survival rates are dependent on temperature, a dependency that is routinely expressed using an analogue of the 0,10 model. This suggestion was made 34 years ago based on 20 survival curves taken from published literature, but has not been revisited since then. The objective of this study was to re-evaluate the accuracy of the Q(10) equation, utilizing data accumulated since 1978. We assembled a database of 450 E. coli survival datasets from 70 peer-reviewed papers. We then focused on the 170 curves taken from experiments that were performed in the laboratory under dark conditions to exclude the effects of sunlight and other field factors that could cause additional variability in results. All datasets were tabulated dependencies "log concentration vs. time." There were three major patterns of inactivation: about half of the datasets had a section of fast log-linear inactivation followed by a section of slow log-linear inactivation; about a quarter of the datasets had a lag period followed by log-linear inactivation; and the remaining quarter were approximately linear throughout. First-order inactivation rate constants were calculated from the linear sections of all survival curves and the data grouped by water sources, including waters of agricultural origin, pristine water sources, groundwater and wells, lakes and reservoirs, rivers and streams, estuaries and seawater, and wastewater. Dependency of E. coli inactivation rates on temperature varied among the water sources. There was a significant difference in inactivation rate values at the reference temperature between rivers and agricultural waters, wastewaters and agricultural waters, rivers and lakes, and wastewater and lakes. At specific sites, the Q(10) equation was more accurate in rivers and coastal waters than in lakes making the value of the Q(10) coefficient appear to be site-specific. Results of this work indicate possible sources of uncertainty to be accounted for in watershed-scale microbial water quality modeling. Published by Elsevier Ltd. C1 [Blaustein, R. A.; Hill, R. L.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Pachepsky, Y.; Shelton, D. R.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Whelan, G.] USEPA Ecosyst Res Div, Natl Exposure Res Lab, Athens, GA USA. RP Pachepsky, Y (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM Yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 FU United States Environmental Protection Agency (gs1) through its Office of Research and Development [DW-12-92348101] FX The United States Environmental Protection Agency (gs1) through its Office of Research and Development partially funded and collaborated in the research described here under contract DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 48 TC 40 Z9 41 U1 3 U2 91 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD FEB 1 PY 2013 VL 47 IS 2 BP 569 EP 578 DI 10.1016/j.watres.2012.10.027 PG 10 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 091TL UT WOS:000315072600010 PM 23182082 ER PT J AU Kasel, D Bradford, SA Simunek, J Heggen, M Vereecken, H Klumpp, E AF Kasel, Daniela Bradford, Scott A. Simunek, Jiri Heggen, Marc Vereecken, Harry Klumpp, Erwin TI Transport and retention of multi-walled carbon nanotubes in saturated porous media: Effects of input concentration and grain size SO WATER RESEARCH LA English DT Article DE Carbon nanotubes; Column experiments; Quartz sand; Breakthrough curves; Retention profiles; Transport modeling ID FULLERENE C-60 NANOPARTICLES; COLLOID TRANSPORT; HYDROXYAPATITE NANOPARTICLES; CRYPTOSPORIDIUM OOCYSTS; AGGREGATION KINETICS; BACTERIA TRANSPORT; FILTRATION THEORY; FLOW CONDITIONS; HUMIC-ACID; DEPOSITION AB Water-saturated column experiments were conducted to investigate the effect of input concentration (C-o) and sand grain size on the transport and retention of low concentrations (1, 0.01, and 0.005 mg L-1) of functionalized C-14-labeled multi-walled carbon nanotubes (MWCNT) under repulsive electrostatic conditions that were unfavorable for attachment. The breakthrough curves (BTCs) for MWCNT typically did not reach a plateau, but had an asymmetric shape that slowly increased during breakthrough. The retention profiles (RPs) were not exponential with distance, but rather exhibited a hyper-exponential shape with greater retention near the column inlet. The collected BTCs and RPs were simulated using a numerical model that accounted for both time- and depth-dependent blocking functions on the retention coefficient. For a given C-o, the depth-dependent retention coefficient and the maximum solid phase concentration of MWCNT were both found to increase with decreasing grain size. These trends reflect greater MWCNT retention rates and a greater number of retention locations in the finer textured sand. The fraction of the injected MWCNT mass that was recovered in the effluent increased and the RPs became less hyper-exponential in shape with higher C-o due to enhanced blocking/filling of retention locations. This concentration dependency of MWCNT transport increased with smaller grain size because of the effect of pore structure and MWCNT shape on MWCNT retention. In particular, MWCNT have a high aspect ratio and we hypothesize that solid phase MWCNT may create a porous network with enhanced ability to retain particles in smaller grain sized sand, especially at higher C-o. Results demonstrate that model simulations of MWCNT transport and fate need to accurately account for observed behavior of both BTCs and RPs. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Kasel, Daniela; Vereecken, Harry; Klumpp, Erwin] Forschungszentrum Julich, Agrosphere Inst IBG 3, D-52425 Julich, Germany. [Bradford, Scott A.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Heggen, Marc] Forschungszentrum Julich, Peter Grunberg Inst PGI 5, D-52425 Julich, Germany. [Heggen, Marc] Forschungszentrum Julich, ER C, D-52425 Julich, Germany. RP Kasel, D (reprint author), Forschungszentrum Julich, Agrosphere Inst IBG 3, D-52425 Julich, Germany. EM d.kasel@fz-juelich.de; scott.bradford@ars.usda.gov; jiri.simunek@ucr.edu; m.heggen@fz-juelich.de; h.vereecken@fz-juelich.de; e.klumpp@fz-juelich.de RI Heggen, Marc/K-5099-2013 OI Heggen, Marc/0000-0002-2646-0078 FU German Federal Ministry of Education and Research FX This research was performed within the framework of the 'NanoFlow'-project supported by the German Federal Ministry of Education and Research. Analysis of bromide in the liquid samples by Stephan Koppchen is highly appreciated. The technical assistance of Herbert Philipp is gratefully acknowledged. We also thank Wolfgang Schierenberg for the determination of the critical coagulation concentration. NR 59 TC 49 Z9 51 U1 8 U2 117 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD FEB 1 PY 2013 VL 47 IS 2 BP 933 EP 944 DI 10.1016/j.watres.2012.11.019 PG 12 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 091TL UT WOS:000315072600045 PM 23228890 ER PT J AU Wang, DJ Paradelo, M Bradford, SA Peijnenburg, WJGM Chu, LY Zhou, DM AF Wang, Dengjun Paradelo, Marcos Bradford, Scott A. Peijnenburg, Willie J. G. M. Chu, Lingyang Zhou, Dongmei TI Facilitated transport of Cu with hydroxyapatite nanoparticles in saturated sand: Effects of solution ionic strength and composition (vol 45, pg 5905, 2011) SO WATER RESEARCH LA English DT Correction C1 [Wang, Dengjun; Chu, Lingyang; Zhou, Dongmei] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. [Paradelo, Marcos] Univ Vigo, Fac Sci, Dept Plant Biol & Soil Sci, Soil Sci Grp, E-32004 Orense, Spain. [Bradford, Scott A.] Agr Res Serv, US Salin Lab, USDA, Riverside, CA 92507 USA. [Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Lab Ecol Risk Assessment, NL-3720 BA Bilthoven, Netherlands. [Peijnenburg, Willie J. G. M.] Leiden Univ, Inst Environm Sci CML, NL-2300 RA Leiden, Netherlands. [Chu, Lingyang] Anhui Agr Univ, Coll Resources & Environm Sci, Hefei 230036, Peoples R China. [Wang, Dengjun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. RP Zhou, DM (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. EM dmzhou@issas.ac.cn RI Wang, Dengjun/H-3171-2012; Peijnenburg, Willie/M-4110-2013; Paradelo, Marcos/J-7077-2015 OI Paradelo, Marcos/0000-0002-2768-0136 NR 1 TC 0 Z9 0 U1 2 U2 51 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD FEB 1 PY 2013 VL 47 IS 2 BP 954 EP 954 DI 10.1016/j.watres.2012.10.007 PG 1 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 091TL UT WOS:000315072600047 ER PT J AU Pollina, JB Colle, BA Charney, JJ AF Pollina, Joseph B. Colle, Brian A. Charney, Joseph J. TI Climatology and Meteorological Evolution of Major Wildfire Events over the Northeast United States SO WEATHER AND FORECASTING LA English DT Article ID FIRES AB This study presents a spatial and temporal climatology of major wildfire events, defined as >100 acres burned (>40.47 ha, where 1 ha = 2.47 acre), in the northeast United States from 1999 to 2009 and the meteorological conditions associated with these events. The northeast United States is divided into two regions: region 1 is centered over the higher terrain of the northeast United States and region 2 is primarily over the coastal plain. About 59% of all wildfire events in these two regions occur in April and May, with similar to 76% in region 1 and similar to 53% in region 2. There is large interannual variability in wildfire frequency, with some years having 4-5 times more fire events than other years. The synoptic flow patterns associated with northeast United States wildfires are classified using the North American Regional Reanalysis. The most common synoptic pattern for region 1 is a surface high pressure system centered over the northern Appalachians, which occurred in approximately 46% of all events. For region 2, the prehigh anticyclone type extending from southeast Canada and the Great Lakes to the northeast United States is the most common pattern, occurring in about 46% of all events. A trajectory analysis highlights the influence of large-scale subsidence and decreasing relative humidity during the events, with the prehigh pattern showing the strongest subsidence and downslope drying in the lee of the Appalachians. C1 [Pollina, Joseph B.; Colle, Brian A.] SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA. [Charney, Joseph J.] US Forest Serv, USDA, E Lansing, MI USA. RP Colle, BA (reprint author), SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA. EM brian.colle@stonybrook.edu FU Stony Brook University; U.S. Forest Service [08-JV-11242306-093] FX This work was supported by a research joint venture agreement between Stony Brook University and the U.S. Forest Service (08-JV-11242306-093). We thank the three anonymous reviewers for their comments and suggestions. NR 29 TC 5 Z9 5 U1 2 U2 8 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0882-8156 J9 WEATHER FORECAST JI Weather Forecast. PD FEB PY 2013 VL 28 IS 1 BP 175 EP 193 DI 10.1175/WAF-D-12-00009.1 PG 19 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 098HT UT WOS:000315540800009 ER PT J AU Verdonk, JC Sullivan, ML AF Verdonk, Julian C. Sullivan, Michael L. TI Artificial microRNA (amiRNA) induced gene silencing in alfalfa (Medicago sativa) SO BOTANY-BOTANIQUE LA English DT Article DE alfalfa; gene silencing; amiRNA; medicago; legume ID LIGNIN STRUCTURE; DOWN-REGULATION; RED-CLOVER; PLANTS; RNA; EXPRESSION; ARABIDOPSIS; BIOSYNTHESIS; EFFICIENT; PATHWAYS AB Gene silencing is a powerful technique that allows the study of the function of specific genes by selectively reducing their transcription. Several different approaches can be used, however they all have in common the artificial generation of single stranded small ribonucleic acids (RNAs) that are utilized by the endogenous gene silencing machinery of the organism. Artificial microRNAs (amiRNA) can be used to very specifically target genes for silencing because only a short sequence of 21 nucleotides of the gene of interest is used. Gene silencing via amiRNA has been developed for Arabidopsis thaliana (L.) Heynh. and rice using endogenous microRNA (miRNA) precursors and has been shown to also work effectively in other dicot species using the arabidopsis miRNA precursor. Here, we demonstrate that the arabidopsis miR319 precursor can be used to silence genes in the important forage crop species alfalfa (Medicago sativa L.) by silencing the expression of a transgenic beta-glucuronidase (GUSPlus) target gene. C1 [Verdonk, Julian C.; Sullivan, Michael L.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Sullivan, ML (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM michael.sullivan@ars.usda.gov OI Verdonk, Julian/0000-0002-1237-7951 NR 36 TC 2 Z9 2 U1 5 U2 31 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD FEB PY 2013 VL 91 IS 2 BP 117 EP 122 DI 10.1139/cjb-2012-0166 PG 6 WC Plant Sciences SC Plant Sciences GA 093QJ UT WOS:000315206200008 ER PT J AU Sugiyama, A Bakker, MG Badri, DV Manter, DK Vivanco, JM AF Sugiyama, Akifumi Bakker, Matthew G. Badri, Dayakar V. Manter, Daniel K. Vivanco, Jorge M. TI Relationships between Arabidopsis genotype-specific biomass accumulation and associated soil microbial communities SO BOTANY-BOTANIQUE LA English DT Article DE Arabidopsis; microbiome; pyrosequencing; plant growth-promoting rhizobacteria; PGPR ID RHIZOSPHERE; GROWTH; ROOTS AB Rhizosphere microbial communities are impacted by resident plant species and have reciprocal effects on their host plants. We collected resident soil from five wild populations of Arabidopsis in the United States and Europe in an effort to characterize the impacts of natural soil microbiomes on Arabidopsis growth performance. The microbial communities present in these soils showed differences in community structure as assessed by 454 sequencing and in metabolic activity. While pathogens associated with the Brassica family were rare, diverse genera of potential plant growth promoting rhizobacteria were detected. Seed corresponding to the five Arabidopsis genotypes was grown in resident and nonresident soils to determine relationships among plant growth performance and soil microbial community and edaphic characteristics. Arabidopsis genotypes demonstrated different patterns of relationship between biomass accumulation and microbial community characteristics. This work sheds light on the bacterial populations naturally associated with Arabidopsis and suggests implications of the rhizosphere microbiome for plant growth performance. C1 [Sugiyama, Akifumi; Bakker, Matthew G.; Badri, Dayakar V.; Vivanco, Jorge M.] Colorado State Univ, Dept Hort & Landscape Architecture, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. [Manter, Daniel K.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. RP Manter, DK (reprint author), USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. EM daniel.manter@ars.usda.gov; j.vivanco@colostate.edu RI Bakker, Matthew/B-8610-2013 OI Bakker, Matthew/0000-0003-0345-0587 FU National Science Foundation [MCB-0950857]; JSPS; USDA NIFA AFRI Postdoctoral Fellowship grant [2011-67012-30938] FX This research was supported by a grant from the National Science Foundation to J.M.V. (MCB-0950857). We thank Alison Anastasio, Diane Byers, Mick Crawley, Joel Kniskern, and Fabrice Roux for collecting soils, and Joy Bergelson for helping to coordinate soil collection. A.S. was supported by a JSPS Postdoctoral Fellowship for Research Abroad. M.B. is supported by a USDA NIFA AFRI Postdoctoral Fellowship grant (2011-67012-30938). NR 21 TC 11 Z9 11 U1 2 U2 59 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD FEB PY 2013 VL 91 IS 2 BP 123 EP 126 DI 10.1139/cjb-2012-0217 PG 4 WC Plant Sciences SC Plant Sciences GA 093QJ UT WOS:000315206200009 ER PT J AU Schmidt, MA Gonzalez, JM Halvorson, JJ Hagerman, AE AF Schmidt, Michael A. Gonzalez, Javier M. Halvorson, Jonathan J. Hagerman, Ann E. TI Metal mobilization in soil by two structurally defined polyphenols SO CHEMOSPHERE LA English DT Article DE Phytochelation; Metal mobilization; Tannin; Polyphenol; Phytoremediation ID CLOUD-POINT EXTRACTION; PHENOLIC-COMPOUNDS; TANNIC-ACID; CONDENSED TANNINS; AQUEOUS-SOLUTION; TRACE-METALS; WASTE-WATER; ECOSYSTEMS; ADSORPTION; IONS AB Polyphenols including tannins comprise a large percentage of plant detritus such as leaf litter, and affect soil processes including metal dynamics. We tested the effects of tannins on soil metal mobilization by determining the binding stoichiometries of two model polyphenols to AI(III) and Fe(III) using micelle-mediated separation and inductively coupled plasma optical emission spectroscopy (ICP-OES). By fitting the data to the Langmuir model we found the higher molecular weight polyphenol (oenothein B) was able to bind more metal than the smaller polyphenol (epigallocatechin gallate, EGCg). For example, oenothein B bound 9.43 mol Fe mol(-1), while EGCg bound 4.41 mol of Fe mol(-1). Using the parameters from the binding model, we applied the Langmuir model for competitive binding to predict binding for mixtures of Al(III) and Fe(III). Using the parameters from the single metal experiments and information about polyphenol sorption to soils we built a model to predict metal mobilization from soils amended with polyphenols. We tested the model with three natural soils and found that it predicted mobilization of Fe and Al with r(2) = 0.92 and r(2) = 0.88, respectively. The amount of metal that was mobilized was directly proportional to the maximum amount of metal bound to the polyphenol. The secondary parameter in each model was the amount of weak organically chelated Fe or Al that was in the soil. This study provides the first compound-specific information about how natural polyphenols interact with metals in the environment. We propose a model that is applicable to developing phytochelation agents for metal detoxification, and we discuss how tannins may play a role in metal mobilization from soils. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Schmidt, Michael A.; Hagerman, Ann E.] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. [Gonzalez, Javier M.] ARS, Natl Soil Eros Res Lab, USDA, W Lafayette, IN 47907 USA. [Halvorson, Jonathan J.] ARS, No Great Plains Res Lab, USDA, Mandan, ND 58554 USA. RP Hagerman, AE (reprint author), Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. EM hagermae@muohio.edu FU USDA [58-1932-6-634] FX This work was funded by USDA Specific Cooperative Agreement 58-1932-6-634 to Miami University. The authors thank J. Harrah, J. Bos and K. Fisher for their excellent analytical assistance. NR 49 TC 4 Z9 8 U1 2 U2 39 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 J9 CHEMOSPHERE JI Chemosphere PD FEB PY 2013 VL 90 IS 6 BP 1870 EP 1877 DI 10.1016/j.chemosphere.2012.10.010 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 092PB UT WOS:000315133400015 PM 23149187 ER PT J AU Delgado, JA Kowalski, K Tebbe, C AF Delgado, Jorge A. Kowalski, Kevin Tebbe, Caleb TI The first Nitrogen Index app for mobile devices: Using portable technology for smart agricultural management SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Smartphone; App; Tablet Nitrogen Index; Nutrient management; Portable agricultural technology ID LOSSES AB Nitrogen fertilizer from organic and inorganic sources is used across the world's agroecosystems. It contributes to higher yields and higher economic returns to farmers, and is essential for food security. However, when more is applied than necessary, significant amounts of nitrogen can exit the system and impact the environment. Appropriate nitrogen management, where the right amounts of nitrogen are applied and best practices are used to ensure higher use efficiency, is important for conservation. A recent study from the USDA reported that about 66% of US cropland was not meeting all of three best management practices for nitrogen: best rate, best time of application, and best method of application (http://www.ers.usda.gov/publications/err-economic-research-report/err127.aspx). Nitrogen tools can help us assess the risk of nitrogen losses from a field to the environment and increase nitrogen use efficiencies (http://www.ars.usda.gov/is/AR/archive/sep11/nitrogen0911.htm). We need quick and mobile nitrogen management risk assessment tools that can be carried to the field and elsewhere. The first smartphone/tablet application (or "app") of the Nitrogen Index was released on February 26th, 2012. A PC (R) version(1) of the Nitrogen Index, which can be used on PC desktop and laptop computers, is also available, so users can email the results from their Nitrogen Index app developed for smartphones/tablets in the field to their computer back at the office (or a farmer's or consultant's computer) using their portable device's internet service. The Nitrogen Index smartphone/tablet application can be downloaded at no cost from the Google Play (TM) website (https://play.google.com/store) to any phone that has the Android no system. To find the application, the user simply needs to do a search at the Google Play website using "Nitrogen Index" as the search term. This new advancement in portable technology will enable the use of small devices such as smartphones to conduct an assessment on site and visit with a farmer at any given field site where a wireless connection is available. The Nitrogen Index is a quick tool, and its assessments of nitrogen loss risk are correlated with observed values. This is the first Nitrogen Index app in the world and it is the beginning of more advances to come in the field of portable agricultural technology. New advances in software (e.g., apps) and technology are bringing us to a new frontier of technology transfer, and portable technologies (e.g., smartphones, tablets) are making possible the development of 'smart agriculture'. Published by Elsevier B.V. C1 [Delgado, Jorge A.; Kowalski, Kevin; Tebbe, Caleb] ARS, Soil Plant Nutrient Res Unit, USDA, Ft Collins, CO 80526 USA. RP Delgado, JA (reprint author), ARS, Soil Plant Nutrient Res Unit, USDA, 2150 Ctr Ave,Bldg D,Suite 100, Ft Collins, CO 80526 USA. EM jorge.delgado@ars.usda.gov NR 8 TC 13 Z9 13 U1 1 U2 79 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD FEB PY 2013 VL 91 BP 121 EP 123 DI 10.1016/j.compag.2012.12.008 PG 3 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 094IF UT WOS:000315255500014 ER PT J AU Lampson, BD Han, YJ Khalilian, A Greene, J Mankin, RW Foreman, EG AF Lampson, B. D. Han, Y. J. Khalilian, A. Greene, J. Mankin, R. W. Foreman, E. G. TI Automatic detection and identification of brown stink bug, Euschistus servus, and southern green stink bug, Nezara viridula, (Heteroptera: Pentatomidae) using intraspecific substrate-borne vibrational signals SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Bioacoustic recognition; Neural network; Mixture model ID ACOUSTIC DETECTION; SINGING INSECTS; COMMUNICATION; SONGS; HEMIPTERA; PLANTS AB Stink bugs cost the southeastern US cotton industry millions of dollars each year in crop losses and control costs. These losses are reduced by strategic pesticide applications; however, current methods of monitoring these pests for making management decisions are time-consuming and costly. Therefore, improved methods to identify and monitor these bugs must be investigated in order to optimize pesticide applications. One such method would be to exploit the substrate-borne vibrational signals (SBVSs) of these insects. Recordings of SBVS for two prevalent regional pests, the brown stink bug, Euschistus servus, and southern green stink bug, Nezara viridula, were segmented into separate pulses of variable duration based on signal energy. For each pulse, the linear frequency cepstral coefficients, dominant frequency, and duration were calculated and used as features. These features were classified using a Gaussian mixture model (GMM) and a probabilistic neural network (PNN) to discriminate these SBVS from incidental sounds and SBVS of different species from each other. Detection of SBVS generated by brown stink bugs was performed with over 92% accuracy for single male-female pairs with both PNN and GMM and with over 86% accuracy for 30 individuals with both PNN and GMM. Detection of SBVS generated by southern green stink bugs was performed with up to 82.5% accuracy with PNN and 68.0% accuracy with GMM for 30 individuals. Also, both PNN and GMM were over 90% accurate in identifying SBVS of brown and southern green stink bugs. Concurrent detection of SBVS from noise and identification of SBVS of brown and southern green stink bugs was 83.3% accurate using PNN and 71.5% accurate using GMM. These results indicated the capability of detecting and identifying stink bug species using their SBVS. Published by Elsevier B.V. C1 [Lampson, B. D.; Han, Y. J.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Khalilian, A.; Greene, J.] Edisto Res & Educ Ctr, Sch Agr Forest & Environm Sci, Blackville, SC 29817 USA. [Mankin, R. W.; Foreman, E. G.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Lampson, BD (reprint author), Clemson Univ, Sch Agr Forest & Environm Sci, 231 McAdams Hall, Clemson, SC 29634 USA. EM bdettma@clemson.edu FU NIFA/USDA [SC-1700289] FX Technical Contribution No. 6043 of the Clemson University Experiment Station. This material is based upon work supported by NIFA/USDA, under Project number SC-1700289. NR 22 TC 3 Z9 3 U1 2 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD FEB PY 2013 VL 91 BP 154 EP 159 DI 10.1016/j.compag.2012.12.010 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 094IF UT WOS:000315255500018 ER PT J AU Eggleston, G Gober, J St Cyr, E AF Eggleston, Gillian Gober, Jessica St Cyr, Eldwin TI Development of an industrial method to quantitatively measure carry-over amylase activity in raw and refined sugars SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE analytical method; carry-over alpha-amylase activity; Bacillus licheniformis; Phadebas (R) tablets; raw sugar; refined cane sugar ID IUPAC TECHNICAL REPORT; ALPHA-AMYLASE; BACILLUS-LICHENIFORMIS; MANUFACTURE; OPTIMIZATION; SUBSTRATE AB In recent years, there has been increased concern over carry-over activity of mostly high temperature (HT) and very high temperature (VHT) stable amylases in white, refined sugars from refineries to various food manufacturing industries and other end-users. HT and VHT stable amylases were developed for much larger markets than the sugar industry with harsher processing conditions. There was an urgent need for an industrial method to measure carry-over amylase in crystalline sugar products. A selective method has been developed using Phadebas (R) blue starch, which is highly specific for a-amylase activity, has few reagents, and only uses existing equipment at the factory and refinery. Raw or refined cane sugars are diluted 60:40 w/v in calcium chloride-Iris buffer (pH 6.0) and mixed with a Phadebas (R) tablet and incubated at 37 degrees C for 45 min. The absorbance of the solution after gravity filtration is measured directly on a spectrophotometer at 620 nm. The presence of Ca2+ ions dramatically enhanced the amylase activity. Prolonged incubation past the optimum time of 45 min only reduced the sensitivity. The most accurate results were obtained when a standard curve was generated from standards that included a 60 Brix white beet sucrose solution. When carry-over activity was detected in raw sugars they tended to be higher than in refined, white sugars because less processing time is available to deactivate the enzyme. The mean activity range detected for refined sugars known to have detrimentally affected the texture of starch puddings was 101 to 145 COU/L (Carry Over Units/Liter) and 272 to 552 COU/L for raw sugars. The lower limit of detection for the industrial method was approximately 25 (COU/L). Precision generally improved with increased activities. Relative standard deviations (RSD) for refined sugars ranged from similar to 12 to 20% with no unsatisfactory performance z scores and only similar to 9.5% of z scores were questionable. The best data and performance statistics were observed for a raw sugar of mean activity of 552.3 COU/L and RSD value of 8.4%. C1 [Eggleston, Gillian; Gober, Jessica; St Cyr, Eldwin] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov NR 25 TC 2 Z9 2 U1 1 U2 9 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD FEB PY 2013 VL 115 IS 1370 BP 123 EP 131 PG 9 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA 095AZ UT WOS:000315308200041 ER PT J AU Ammar, E Walter, AJ Hall, DG AF Ammar, El-Desouky Walter, Abigail J. Hall, David G. TI New Excised-Leaf Assay Method to Test Inoculativity of Asian Citrus Psyllid (Hemiptera: Psyllidae) With Candidatus Liberibacter asiaticus Associated With Citrus Huanglongbing Disease SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE huanglongbing; citrus greening; Candidatus Liberibacter asiaticus; Asian citrus psyllid; Diaphorina citri ID CA. L. AMERICANUS; REAL-TIME PCR; DIAPHORINA-CITRI; GREENING DISEASE; VECTOR; IDENTIFICATION; TRANSMISSION; BRAZIL; VIRUS; HOSTS AB The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the primary vector of Candidatus Liberibacter asiaticus (Las) associated with huanglongbing, or citrus greening, the most devastating citrus (Citrus spp.) disease worldwide. Here, we developed a new "excised-leaf assay" that can speed up Las-inoculativity tests on Asian citrus psyllid from the current 3-12 mo (when using whole citrus seedlings for inoculation) to only 2-3 wk. Young adults of Asian citrus psyllid that had been reared on Las-infected plants were caged on excised healthy sweet orange [Citrus sinensis (L.) Osbeck] leaves for a 1-2-wk inoculation access periods (IAP), and then both psyllids and leaves were tested later by quantitative polymerase chain reaction (PCR). When single adults were tested per leaf, percentages of Las-positive leaves averaged 2-6% by using HLBaspr primers and 10-20% by using the more sensitive LJ900 primers. Higher proportions of Las-positive leaves were obtained with 1) higher densities of inoculating psyllids (5-10 adults per leaf), 2) longer IAPs, and 3) incubation of leaves for 1 wk postinoculation before PCR. Logistic regression analysis indicated a positive correlation between Las titer in Asian citrus psyllid adults tested singly and the probability of detecting Las in the inoculated leaves, correlations that can be very useful in epidemiological studies. Comparison between excised leaves and whole seedlings, inoculated consecutively for 1 wk each by one or a group of psyllids, indicated no significant difference between Las detection in excised leaves or whole plants. This new excised-leaf assay method saves considerable time, materials, and greenhouse space, and it may enhance vector relation and epidemiological studies on Las and potentially other Liberibacter spp. associated with huanglongbing disease. C1 [Ammar, El-Desouky; Walter, Abigail J.; Hall, David G.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Ammar, E (reprint author), ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM eldammar@hotmail.com RI Walter, Abigail/F-5574-2012 OI Walter, Abigail/0000-0003-1388-3706 FU Florida Citrus Research and Development Foundation FX We thank Kathy Moulton, Monty Watson, Tina Grigsby Kristen Wald, and Pei-Ling Li for excellent technical assistance and John Kent Morgan (USDA-ARS, Fort Pierce, FL) and two anonymous reviewers for suggestions to improve an earlier version of the manuscript. Funds for this research were provided by the Florida Citrus Research and Development Foundation. NR 34 TC 9 Z9 9 U1 0 U2 42 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 25 EP 35 DI 10.1603/EC12245 PG 11 WC Entomology SC Entomology GA 089BC UT WOS:000314882200004 PM 23448011 ER PT J AU Behle, RW Jackson, MA Flor-Weiler, LB AF Behle, Robert W. Jackson, Mark A. Flor-Weiler, Lina B. TI Efficacy of a Granular Formulation Containing Metarhizium brunneum F52 (Hypocreales: Clavicipitaceae) Microsclerotia Against Nymphs of Ixodes scapularis (Acari: Ixoididae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE microsclerotia; Ixodes scapularis; black-legged tick; Metarhizium brunneum; granule formulation ID FUNGI BEAUVERIA-BASSIANA; TICK BOOPHILUS-MICROPLUS; ENTOMOPATHOGENIC FUNGI; ENTOMOGENOUS FUNGI; AMBLYOMMA-VARIEGATUM; BIOLOGICAL-CONTROL; BIOCONTROL AGENT; ANISOPLIAE; IXODIDAE; PATHOGENICITY AB Technical improvements in the production and formulation of microbial agents will increase the potential for development of biological pesticides that are able to compete with chemical insecticides in the marketplace. Here we report the efficacy of a simple granule formulation containing microsclerotia of Metarhizium brunneum (Petch) (Hypocreales: Clavicipitaceae) for control of unfed and fed nymphs of Ixodes scpaularis Say (Acari: Ixoididae). Microsclerotial granules of M. brunneum applied to moist potting mix produce infective conidia within 2 wk and conidia remained viable for up to 8 wk after application. Microsclerotial granules produced from 3.05 x 10(9) to 1.24 x 10(10) conidia g(-1) granules in potting mix. Both unfed and fed nymphs were susceptible to infection when exposed to treated potting soil with up to 56 and 74% mortality, respectively. M. brunneum demonstrated a transtadial infection for fed nymphs exposed to treated potting mix with signs of a fungal infection becoming apparent only after molting into adults. High conidial production rates from microsclerotial granules of M. brunneum combined with significant tick mortality support the need for additional research to evaluate the efficacy of this treatment technology as a biopesticide option for control of ticks. C1 [Behle, Robert W.; Jackson, Mark A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Flor-Weiler, Lina B.] Marrone Bio Innovat Inc, Davis, CA 95618 USA. RP Behle, RW (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM robert.behle@ars.usda.gov NR 34 TC 10 Z9 10 U1 1 U2 40 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 57 EP 63 DI 10.1603/EC12226 PG 7 WC Entomology SC Entomology GA 089BC UT WOS:000314882200008 PM 23448015 ER PT J AU Sampson, BJ Roubos, CR Stringer, SJ Marshall, D Liburd, OE AF Sampson, Blair J. Roubos, Craig R. Stringer, Stephen J. Marshall, Donna Liburd, Oscar E. TI Biology and Efficacy of Aprostocetus (Eulophidae: Hymenoptera) as a Parasitoid of the Blueberry Gall Midge Complex: Dasineura oxycoccana and Prodiplosis vaccinii (Diptera: Cecidomyiidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biological control; Platygastridae; Eulophidae; Vaccinium; parasitism ID DIAPREPES-ABBREVIATUS COLEOPTERA; CURCULIONIDAE; CRANBERRY; PEST AB In the southeastern United States, bud-infesting larvae of two gall midge species, Dasineura oxycoccana (Johnson) and Prodiplosis vaccinii (Felt), destroy from 20 to 80% of the rabbiteye blueberry crop, Vaccinium virgatum Aiton (syn. V. ashei Reade). These midge larvae are attacked by five species of parasitoid wasps. The most effective of these is the bivoltine eulophid Aprostocetus sp. nr. marylandensis (Eulophidae), whose adults constitute one-third of the gall midge parasitoids, active in both conventional and organic blueberry fields. Broods of Aprostocetus use several reproductive strategies to keep sole possession of their larval hosts. As solitary endoparasitoids as well as facultative hyperparasitoids, precocial larvae of Aprostocetus devour hosts organs along with any younger siblings and rival parasitoid broods. Although larger hosts are preferred, any sized larvae can be parasitized, which reduces brood congestion and infanticide. An Aprostocetus female spends an hour or more in a systematic hunt for hosts, during which time 40 to 100% of midge larvae encountered are parasitized. Aprostocetus females could have located hosts more quickly had they recognized host-feeding scars as cues. Even so, high rates of larval parasitism achieved by Aprostocetus may kill as many midges as insecticides do. C1 [Sampson, Blair J.; Stringer, Stephen J.; Marshall, Donna] ARS, USDA, Thad Cochran So Hort Lab, Poplarville, MS 39470 USA. [Roubos, Craig R.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Liburd, Oscar E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Sampson, BJ (reprint author), ARS, USDA, Thad Cochran So Hort Lab, 810 Hwy 26 W, Poplarville, MS 39470 USA. EM blair.sampson@ars.usda.gov FU USDA Pest Management Alternatives Grant (PMAP) [731497113] FX This research was funded in part by a USDA Pest Management Alternatives Grant (PMAP) No. 731497113. The authors are grateful to many cooperating blueberry growers: Jerry and Angie Hutto, Luis Monterde, JC Paceria, John Kattengall, and Mandy and Colleen McCormick. NR 32 TC 2 Z9 2 U1 3 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 73 EP 79 DI 10.1603/EC12404 PG 7 WC Entomology SC Entomology GA 089BC UT WOS:000314882200010 PM 23448017 ER PT J AU Cooper, WR Spurgeon, DW AF Cooper, W. Rodney Spurgeon, Dale W. TI Temperature-Dependent Egg Development of Lygus hesperus (Hemiptera: Miridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biophysical model; temperature-dependent development; western tarnished plant bug; degree-day ID TARNISHED PLANT BUG; NYMPHS AB Lygus hesperus Knight (Hemiptera: Miridae) is a key agricultural pest in the western United States, but certain aspects of its temperature-dependent development are poorly defined. Accurate models describing the relationships between temperature and development of L. hesperus would facilitate the study of Lygus seasonal population dynamics and overwintering ecology. We used nonlinear biophysical models to describe the development of L. hesperus eggs oviposited in green bean pods and semitransparent agarose and held under constant temperatures from 10 to 37.8 degrees C. The agarose substrate was used because it is less susceptible than green beans to deterioration at low and high temperature extremes, and because it allowed the observation of phases of embryonic development that are hidden from view from eggs developing in plant material. The models indicated that both low and high temperature inhibited development of each observed embryonic phase regardless of oviposition substrate. Developmental rates asymptotically approached zero with decreasing temperature in the lower thermal range, and decreased with increasing temperatures above 32.2 degrees C. Eggs oviposited in agarose developed slower than eggs oviposited in green bean pods suggesting that egg developmental rates were influenced by the type of host substrate. Our temperature-dependent developmental rate models for L. hesperus eggs supplement nonlinear models recently reported for L. hesperus nymphal and adult reproductive development and should be useful in planning and interpreting field studies of L. hesperus population dynamics and overwintering ecology. C1 [Cooper, W. Rodney; Spurgeon, Dale W.] ARS, USDA, Western Integrated Cropping Syst Res Unit, Shafter, CA 93263 USA. RP Cooper, WR (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 16 TC 4 Z9 4 U1 0 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 124 EP 130 DI 10.1603/EC12311 PG 7 WC Entomology SC Entomology GA 089BC UT WOS:000314882200016 PM 23448023 ER PT J AU Lee, DH Wright, SE Leskey, TC AF Lee, Doo-Hyung Wright, Starker E. Leskey, Tracy C. TI Impact of Insecticide Residue Exposure on the Invasive Pest, Halyomorpha halys (Hemiptera: Pentatomidae): Analysis of Adult Mobility SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE brown marmorated stink bug; invasive species; pesticide; movement; sublethal ID MARMORATED STINK BUG; EUSCHISTUS-SERVUS; STAL HETEROPTERA; APPLE ORCHARDS; TOXICITY; BROWN; SUSCEPTIBILITY; BEHAVIOR; DIPTERA AB Twenty eight insecticides were evaluated in the laboratory to characterize the impact of specific compounds on locomotory behavior and mobility of adult Halyomorpha halys (Stal) (Hemiptera: Pentatomidae). Horizontal distance and angular velocity were measured for individuals exposed to dry insecticide residue for 4.5 h to evaluate how quickly and intensely a given insecticide induced changes in motor activities in no-choice glass arenas. Eight out of nine pyrethroid insecticides induced uncoordinated and irregular movement within 10 min after exposure to insecticides. After 1.5 h, most adults were incapacitated. By contrast, there was no immediate stimulation when H. halys were exposed to organophosphate residues. After 1.5 h, four out of seven organophosphates resulted in increased horizontal distance moved and angular velocity indicating irregular walking paths by exposed adults. Carbamate and neonicotinoid insecticides produced fairly similar patterns with virtually no stimulation in horizontal distance moved or angular velocity, except for imidacloprid and thiacloprid. Neither endosulfan (organochlorine) nor indoxacarb (oxadiazine) affected the horizontal movement of H. halys. Vertical distance climbed by adult H. halys was measured immediately after the 4.5-h insecticide exposure period and at 7 d. In general, adults that survived until day 7 were able to climb vertical distances similar to those in the control. In particular, this result was observed for seven out of nine pyrethroid materials that incapacitated all adults after the 4.5-h exposure period. Mobility changes of adult H. halys are discussed in the context of enhancing integrated pest management programs. C1 [Lee, Doo-Hyung; Wright, Starker E.; Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Lee, DH (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM doohyung.lee@ars.usda.gov FU United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) Specialty Crop Research Initiative (SCRI) [2011-51181-30937] FX We thank Cameron Scorza, Torri Hancock, Brent Short, and John Cullum for excellent technical support. This work was supported in part by United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) Specialty Crop Research Initiative (SCRI) 2011-51181-30937 award, and specific cooperative agreements with USDA-Animal and Plant Health Inspection Service (USDA-APHIS) and the Pennsylvania State University. NR 29 TC 13 Z9 14 U1 2 U2 50 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 150 EP 158 DI 10.1603/EC12265 PG 9 WC Entomology SC Entomology GA 089BC UT WOS:000314882200020 PM 23448027 ER PT J AU Ranger, CM Reding, ME Oliver, JB Moyseenko, JJ Youssef, N Krause, CR AF Ranger, Christopher M. Reding, Michael E. Oliver, Jason B. Moyseenko, James J. Youssef, Nadeer Krause, Charles R. TI Acute Toxicity of Plant Essential Oils to Scarab Larvae (Coleoptera: Scarabaeidae) and Their Analysis by Gas Chromatography-Mass Spectrometry SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE essential oils; botanical insecticides; GC-MS; white grubs; scarab larvae ID EUROPEAN CHAFER COLEOPTERA; WHITE GRUBS COLEOPTERA; ORIENTAL BEETLE; JAPANESE-BEETLE; INSECTICIDAL PROPERTIES; BOTANICAL INSECTICIDES; ROSMARINUS-OFFICINALIS; SPODOPTERA-LITURA; TOBACCO CUTWORM; EFFICACY AB Larvae of scarab beetles (Coleoptera: Scarabaeidae) are important contaminant and root-herbivore pests of ornamental crops. To develop alternatives to conventional insecticides, 24 plant-based essential oils were tested for their acute toxicity against third instars of the Japanese beetle Popillia japonica Newman, European chafer Rhizotrogus majalis (Razoumowsky), oriental beetle Anomala orientalis (Waterhouse), and northern masked chafer Cyclocephala borealis Arrow. Diluted solutions were topically applied to the thorax, which allowed for calculating LD50 and LD90 values associated with 1 d after treatment. A wide range in acute toxicity was observed across all four scarab species. Of the 24 oils tested, allyl isothiocyanate, cinnamon leaf, clove, garlic, and red thyme oils exhibited toxicity to all four species. Allyl isothiocyanate was the most toxic oil tested against the European chafer, and among the most toxic against the Japanese beetle, oriental beetle, and northern masked chafer. Red thyme was also comparatively toxic to the Japanese beetle, oriental beetle, European chafer, and northern masked chafer. Interspecific variability in susceptibility to the essential oils was documented, with 12, 11, 8, and 6 of the 24 essential oils being toxic to the oriental beetle, Japanese beetle, European chafer, and northern masked chafer, respectively. Analysis of the active oils by gas chromatography-mass spectrometry revealed a diverse array of compounds, mostly consisting of mono-and sesquiterpenes. These results will aid in identifying active oils and their constituents for optimizing the development of plant essential oil mixtures for use against scarab larvae. C1 [Ranger, Christopher M.; Reding, Michael E.; Moyseenko, James J.; Krause, Charles R.] ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Dept Entomol, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Oliver, Jason B.; Youssef, Nadeer] Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, McMinnville, TN 37110 USA. RP Ranger, CM (reprint author), ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU ARS Research Project (National Program 304-Crop Protection and Quarantine) [3607-22000-012-00D] FX We thank Jennifer Barnett and Leslie Morris for technical assistance. This research was supported by base funds associated with ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine). NR 57 TC 1 Z9 1 U1 5 U2 46 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 159 EP 167 DI 10.1603/EC12319 PG 9 WC Entomology SC Entomology GA 089BC UT WOS:000314882200021 PM 23448028 ER PT J AU Armstrong, JS Brewer, MJ Parker, RD Adamczyk, JJ AF Armstrong, J. Scott Brewer, Michael J. Parker, Roy D. Adamczyk, J. J., Jr. TI Verde Plant Bug (Hemiptera: Miridae) Feeding Injury to Cotton Bolls Characterized by Boll Age, Size, and Damage Ratings SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Creontiades signatus; verde plant bug; feeding injury; cotton AB The verde plant bug, Creontiades signatus (Distant), has been present in south Texas for several years but has more recently been documented as an economic threat to cultivated cotton, Gossypium hirsutum L. Our studies over 2 yr (2009 and 2010) and two locations (Weslaco and Corpus Christi, TX) investigated feeding-injury of the verde plant bug to a range of cotton boll age classes defined by boll diameter and accumulated degree-days (anthesis to the time of infesting) for first-position cotton bolls infested with the plant bugs. The most detrimental damage to younger cotton bolls from verde plant bug feeding was boll abscission. Cotton bolls <04 accumulating daily degree-days (ACDD), or a boll diameter of 1.3 cm were subject to 60-70% higher boll abscission when compared with the noninfested controls. Significantly higher boll abscission occurred from verde plant bug injured bolls compared with the controls up to 162 ACDD or a mean boll diameter 2.0 cm. Cotton seed weights were significantly reduced up to 179 ACDD or a boll diameter of 2.0 cm at Weslaco in 2009, and up to 317 ACDD or boll diameter 2.6 cm for Weslaco in 2010 when compared with the noninfested controls. Lint weight per cotton boll for infested and noninfested bolls was significantly reduced up to 262 ACDD or boll diameter 2.5 for Corpus Christi in 2010 and up to 288 ACCD or boll diameter 2.6 cm for Weslaco, TX, in 2010. Damage ratings (dependant variable) regressed against infested and noninfested seed-cotton weights showed that in every instance, the infested cotton bolls had a strong and significant relationship with damage ratings for all age classes of bolls. Damage ratings for the infested cotton bolls that did not abscise by harvest showed visual signs of verde plant bug feeding injury and the subsequent development of boll rot; however, these two forms of injury causing lint and seed mass loss are hard to differentiate from open or boll-locked cotton bolls. Based on the results of both lint and seed loss over 2 yr and four studies cotton bolls should be protected up to approximate to 300 ACDD or a boll diameter of 2.5 cm. This equilibrates to bolls that are 12-14 d of age dependent upon daily maximum and minimum temperatures. C1 [Armstrong, J. Scott] ARS, USDA, Beneficial Insects Res Unit, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Brewer, Michael J.; Parker, Roy D.] Texas A&M AgriLife Res & Extens Ctr, Corpus Christi, TX 78406 USA. [Adamczyk, J. J., Jr.] ARS, USDA, Thad Cochran So Hort Lab, So Hort Res Unit, Poplarville, MS 39470 USA. RP Armstrong, JS (reprint author), ARS, USDA, Beneficial Insects Res Unit, Kika de la Garza Subtrop Agr Res Ctr, 2413 E Highway 83, Weslaco, TX 78596 USA. EM scott.armstrong@weslaco.ars.usda.gov FU Cotton incorporated FX We appreciate the dedicated work of J. Martinez, D. Anderson, and Megan Bloemer for technical assistance with this study. We also thank Cotton incorporated for financial support for conducting this study, and J. Goolsby and C. Allen for reviewing this manuscript. NR 10 TC 2 Z9 2 U1 0 U2 7 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 189 EP 195 DI 10.1603/EC12018 PG 7 WC Entomology SC Entomology GA 089BC UT WOS:000314882200024 PM 23448031 ER PT J AU Miller, DR Crowe, CM Barnes, BF Gandhi, KJK Duerr, DA AF Miller, Daniel R. Crowe, Christopher M. Barnes, Brittany F. Gandhi, Kamal J. K. Duerr, Donald A. TI Attaching Lures to Multiple-Funnel Traps Targeting Saproxylic Beetles (Coleoptera) in Pine Stands: Inside or Outside Funnels? SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cerambycidae; Cleridae; Histeridae; Scolytinae; Trogossitidae ID CAPTURING CERAMBYCID BEETLES; SOUTHEASTERN UNITED-STATES; WOOD-BORING INSECTS; ATTRACTANT KAIROMONES; PANEL TRAPS; (-)-ALPHA-PINENE; ETHANOL; CATCHES; BARK AB We conducted two field trapping experiments with multiple-funnel traps in 2008 and one experiment in 2010 to determine the effects of lure placement (inside or outside funnels) on catches of saproxylic species of beetles (Coleoptera). The experiments were conducted in southern pine (Pinus spp.) stands in central Georgia using combinations of ethanol, alpha-pinene, ipsenol, and ipsdienol lures. We report on a modification to the multiple-funnel trap that allows placement of large lures inside the confines of the funnels with minimal blockage. In general, catches of five species of common longhorn beetles (Cerambycidae), two species of regeneration weevils (Curculionidae), four species of bark beetles (Curculionidae: Scolytinae), and seven species of beetle predators and ectoparasites (Cleridae, Histeridae, Tenebrionidae, Trogossitidae, and Zopheridae) were higher in funnel traps with lures attached inside the funnels than in those with lures attached outside of the funnels. Catches of the remaining species were unaffected by lure placement. In no instance were catches of any species lower in funnel traps with lures attached inside the funnels than in those with lures attached outside of the funnels. For most species, catches in modified funnel traps with ethanol, alpha-pinene, ipsenol, and ipsdienol lures attached inside funnels were comparable with those in cross-vane panel traps. C1 [Miller, Daniel R.; Crowe, Christopher M.] USDA, Forest Serv, So Res Stn, Athens, GA 30602 USA. [Barnes, Brittany F.; Gandhi, Kamal J. K.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Duerr, Donald A.] USDA, Forest Serv, Atlanta, GA 30309 USA. RP Miller, DR (reprint author), USDA, Forest Serv, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM dmiller03@fs.fed.us FU USDA Forest Service Special Technology and Development Program (STDP) [R8-2009-03]; Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA FX We thank Jackson Audley and Sean Meadows for lab and field assistance; the staff of the Oconee National Forest for access and field assistance; and Therese Poland, Staffan Lindgren, and two anonymous reviewers for comments on the manuscript. This research was supported in part by a grant (R8-2009-03) from the USDA Forest Service Special Technology and Development Program (STDP) and the Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA. NR 23 TC 14 Z9 14 U1 0 U2 22 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 206 EP 214 DI 10.1603/EC12254 PG 9 WC Entomology SC Entomology GA 089BC UT WOS:000314882200026 PM 23448033 ER PT J AU Progar, RA Blackford, DC Cluck, DR Costello, S Dunning, LB Eager, T Jorgensen, CL Munson, AS Steed, B Rinella, MJ AF Progar, R. A. Blackford, D. C. Cluck, D. R. Costello, S. Dunning, L. B. Eager, T. Jorgensen, C. L. Munson, A. S. Steed, B. Rinella, M. J. TI Population Densities and Tree Diameter Effects Associated With Verbenone Treatments to Reduce Mountain Pine Beetle-Caused Mortality of Lodgepole Pine SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE mountain pine beetle; verbenone; semiochemical; lodgepole pine; antiaggregant ID DENDROCTONUS-BREVICOMIS COLEOPTERA; NONHOST ANGIOSPERM VOLATILES; BRITISH-COLUMBIA; CONIFEROUS FORESTS; UNITED-STATES; BARK BEETLES; PONDEROSAE; ATTACK; CURCULIONIDAE; SCOLYTIDAE AB Mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae), is among the primary causes of mature lodgepole pine, Pinus contorta variety latifolia mortality. Verbenone is the only antiaggregant semiochemical commercially available for reducing mountain pine beetle infestation of lodgepole pine. The success of verbenone treatments has varied greatly in previous studies because of differences in study duration, beetle population size, tree size, or other factors. To determine the ability of verbenone to protect lodgepole pine over long-term mountain pine beetle outbreaks, we applied verbenone treatments annually for 3 to 7 yr at five western United States sites. At one site, an outbreak did not develop; at two sites, verbenone reduced lodgepole pine mortality in medium and large diameter at breast height trees, and at the remaining two sites verbenone was ineffective at reducing beetle infestation. Verbenone reduced mountain pine beetle infestation of lodgepole pine trees in treated areas when populations built gradually or when outbreaks in surrounding untreated forests were of moderate severity. Verbenone did not protect trees when mountain pine beetle populations rapidly increase. C1 [Progar, R. A.] USFS, Pacific NW Res Stn, La Grande, OR 97850 USA. [Blackford, D. C.; Dunning, L. B.; Munson, A. S.] USFS, Ogden, UT 84403 USA. [Cluck, D. R.] USFS, Susanville, CA 96130 USA. [Costello, S.] USFS, Lakewood, CO 80225 USA. [Eager, T.] USFS, Gunnison, CO 81230 USA. [Jorgensen, C. L.] USFS, Boise, ID 83709 USA. [Steed, B.] USFS, Missoula, MT 59807 USA. [Rinella, M. J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Progar, RA (reprint author), USFS, Pacific NW Res Stn, La Grande, OR 97850 USA. EM rprogar@fs.fed.us FU Western Bark Beetle Initiative; USDA Forest Health Protection; Special Technology Development Program FX We thank Chris Fettig (Pacific Southwest Research Station, USDA Forest Service), Dezene Huber (University of Northern British Columbia, Canada), Rick Kelsey (Pacific Northwest Research Station, USDA Forest Service), Jose Negron (Rocky Mountain Research Station, USDA Forest Service), and Lia Spiegel (Blue Mountain Pest Management Service Center, USDA Forest Service), and an unknown reviewer for helpful reviews. We thank the Western Bark Beetle Initiative 2005 for funding to establish sites in Heber, UT, and Stanley Lake, ID. USDA Forest Health Protection and the Special Technology Development Program are especially appreciated for funding and support for sites at Redfish Lake, ID; Alpine, WY; and Bellaire Lake, CO. NR 55 TC 6 Z9 6 U1 0 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 221 EP 228 DI 10.1603/EC12292 PG 8 WC Entomology SC Entomology GA 089BC UT WOS:000314882200028 PM 23448035 ER PT J AU Jones, MI Coleman, TW Graves, AD Flint, ML Seybold, SJ AF Jones, Michael I. Coleman, Tom W. Graves, Andrew D. Flint, Mary Louise Seybold, Steven J. TI Sanitation Options for Managing Oak Wood Infested With the Invasive Goldspotted Oak Borer (Coleoptera: Buprestidae) in Southern California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE debarking; firewood; grinding; oak mortality; solarization ID AGRILUS-PLANIPENNIS COLEOPTERA; EMERALD ASH BORER; COXALIS WATERHOUSE; LONGHORNED BEETLE; FIREWOOD; AUROGUTTATUS; WOODLANDS; SURVIVAL; IMPACTS; LOGS AB Movement of invasive wood-boring insects in wood products presents a threat to forest health and a management challenge for public and private land managers. The goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), is a new pest in San Diego and Riverside Cos., CA, believed to have been introduced on firewood. This beetle has caused elevated levels of oak mortality since 2002. From 2009-2011, we tested several sanitation methods, applicable to large and small land parcels, to reduce or prevent goldspotted oak borer emergence from infested oak wood. In most experiments, emergence of goldspotted oak borer adults from the positive controls demonstrated that the beetle could complete development in firewood-sized pieces of cut oak wood. In 2009, adult emergence from sun-exposed oak wood began and peaked 2- to 4-wks earlier at a low elevation site than at a high elevation site (late May to late June). However, there were no significant effects of elevation or host species on the emergence response of goldspotted oak borer by solarization treatment in this study. Solarization of infested wood with thick (6 mil) and thin (1 mil) plastic tarpaulins (tarps) did not significantly reduce emergence of adults despite recordings of greater mean and maximum daily temperatures in both tarped treatments and greater relative humidity in the thick-tarped treatment (all compared with nontarped controls). Grinding wood with a 3 ''-minus screen (<= 7.6 cm) significantly reduced goldspotted oak borer emergence compared with control treatments, and this was the best method for reducing adult emergence among those tested. In a separate grinding study, no adults emerged when wood was ground to 9 ''-minus (22.9 cm), 2 ''-minus (5.1 cm), or 1 ''-minus (2.5 cm) screen sizes, but a low level of adult emergence from the positive controls limited any inferences from this experiment. Debarking cut wood pieces eliminated goldspotted oak borer emergence from the wood fraction, but adults emerged from the shaved bark and phloem. C1 [Jones, Michael I.; Flint, Mary Louise] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Coleman, Tom W.] USDA, Forest Serv Forest Hlth Protect, San Bernardino, CA 92408 USA. [Graves, Andrew D.] USDA, Forest Serv Forest Hlth Protect, Albuquerque, NM 87102 USA. [Flint, Mary Louise] Statewide Integrated Pest Management Program, Davis, CA 95616 USA. [Seybold, Steven J.] USDA, Forest Serv, Pacific SW Res Stn, Davis, CA 95616 USA. RP Coleman, TW (reprint author), USDA, Forest Serv Forest Hlth Protect, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. EM twcoleman@fs.fed.us FU USDA Forest Service, Pacific Southwest Research Station, Invasive Species Program; Forest Health Protection, Region 5 and Washington Office; University of California, Davis FX The authors would like to thank Stacy M. Hishinuma, Laurel J. Haavik, and Deguang Liu, University of California, Davis, Department of Entomology (UCD); Andreana Cipollone, Grayland Walter, and Paul Zambino, USDA Forest Service, Forest Health Protection, Region 5; Roger Covalt, William Heise County Park; Jeff Robinson and the Glencliff Fire Station, Cleveland National Forest; and the rest of the staff of the Cleveland National Forest for their support of this work. Larry Swan, USDA Forest Service, Pacific Northwest Region 6; Kevin Turner and Tom Scott, University of California, Riverside; Kathleen Edwards, CA Department of Forestry and Fire; Ray Lennox and Nedra Martinez, Cuyamaca Rancho State Park were instrumental in supporting the 2011 grinding studies. We thank Yigen Chen (UCD) for a critical review of the manuscript and both Y. Chen and Paul L. Dallara (also UCD) for key assistance with the statistical analyses. Two anonymous reviewers provided valuable comments to this manuscript. The Cleveland National Forest, Glencliff Fire Station, and Cuyamaca Rancho State Park graciously provided sites to conduct this work. Funding for this work was provided primarily by the USDA Forest Service, Pacific Southwest Research Station, Invasive Species Program; Forest Health Protection, Region 5 and Washington Office; and the University of California, Davis. NR 42 TC 11 Z9 11 U1 1 U2 39 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 235 EP 246 DI 10.1603/EC12177 PG 12 WC Entomology SC Entomology GA 089BC UT WOS:000314882200030 PM 23448037 ER PT J AU Shapiro-Ilan, DI Cottrell, TE Jackson, MA Wood, BW AF Shapiro-Ilan, David I. Cottrell, Ted E. Jackson, Mark A. Wood, Bruce W. TI Control of Key Pecan Insect Pests Using Biorational Pesticides SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE aphid; biorational; Chromobacterium subtsugae; Curculio caryae; Grandevo ID CURCULIO-CARYAE COLEOPTERA; PAECILOMYCES-FUMOSOROSEUS; ENTOMOPATHOGENIC FUNGI; APHID HOMOPTERA; CHROMOBACTERIUM-SUBTSUGAE; STEINERNEMA-CARPOCAPSAE; BIOLOGICAL-CONTROL; MANAGEMENT; CHRYSOMELIDAE; ALEYRODIDAE AB Key pecan insect pests include the black pecan aphid, Melanocallis caryaefoliae (Davis), pecan weevil, Curculio caryae (Horn), and stink bugs (Hemiptera: Pentatomidae). Alternative control tactics are needed for management of these pests in organic and conventional systems. Our objective was to evaluate the potential utility of several alternative insecticides including three plant extract formulations, eucalyptus extract, citrus extract-8.92%, and citrus extract-19.4%, and two microbial insecticides, Chromobacterium subtsugae (Martin et al.) and Isaria fumosorosea (Wize). In the laboratory, eucalyptus extract, citrus extract-8.92%, citrus extract-19.4%, and C. subtsugae caused M. caryaefoliae mortality (mortality was reached approximate to 78, 83, and 96%, respectively). In field tests, combined applications of I. fumosorosea with eucalyptus extract were synergistic and caused up to 82% mortality in M. caryaefoliae. In laboratory assays focusing on C. caryae suppression, C. subtsugae reduced feeding and oviposition damage, eucalyptus extract and citrus extract-19.4% were ineffective, and antagonism was observed when citrus extract-19.4% was combined with the entomopathogenic nematode, Steinernema carpocapsae (Weiser). In field tests, C. subtsugae reduced C. caryae damage by 55% within the first 3 d, and caused 74.5% corrected mortality within 7 d posttreatment. In the laboratory, C. subtsugae and eucalyptus extract did not cause mortality in the brown stink bug, Euschistus servus (Say). Applications of C. subtsugae for suppression of C. caryae, and eucalyptus extract plus I. fumosorosea for control of M. caryaefoliae show promise as alternative insecticides and should be evaluated further. C1 [Shapiro-Ilan, David I.; Cottrell, Ted E.; Wood, Bruce W.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Jackson, Mark A.] ARS, USDA, NCAUR, Peoria, IL 61604 USA. RP Shapiro-Ilan, DI (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM david.shapiro@ars.usda.gov FU Georgia Agricultural Commodity Commission FX We thank A. Amis, T. Brearley, W. Evans, and K. Halat for technical assistance, and the Georgia Agricultural Commodity Commission for funding a portion of the research. Mention of a proprietary product name does not imply USDAs approval of the product to the exclusion of others that may be suitable. NR 49 TC 2 Z9 2 U1 1 U2 46 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 257 EP 266 DI 10.1603/EC12302 PG 10 WC Entomology SC Entomology GA 089BC UT WOS:000314882200032 PM 23448039 ER PT J AU Reding, ME Oliver, JB Schultz, PB Ranger, CM Youssef, NN AF Reding, Michael E. Oliver, Jason B. Schultz, Peter B. Ranger, Christopher M. Youssef, Nadeer N. TI Ethanol Injection of Ornamental Trees Facilitates Testing Insecticide Efficacy Against Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xylosandrus; ornamental nursery; Pyrethroid; plant-based essential oil ID XYLOSANDRUS-GERMANUS COLEOPTERA; BAITED TRAPS; PRIMARY ATTRACTANT; BARK BEETLES; ATTACK; NURSERIES; VOLATILES; CARBARYL; STRESS; NORTH AB Exotic ambrosia beetles are damaging pests in ornamental tree nurseries in North America. The species Xylosandrus crassiusculus (Motshulsky) and Xylosandrus germanus (Blandford) are especially problematic. Management of these pests relies on preventive treatments of insecticides. However, field tests of recommended materials on nursery trees have been limited because of unreliable attacks by ambrosia beetles on experimental trees. Ethanol-injection of trees was used to induce colonization by ambrosia beetles to evaluate insecticides and botanical formulations for preventing attacks by ambrosia beetles. Experiments were conducted in Ohio, Tennessee, and Virginia. Experimental trees injected with ethanol had more attacks by ambrosia beetles than un-injected control trees in all but one experiment. Xylosandrus crassiusculus and X. germanus colonized trees injected with ethanol. In most experiments, attack rates declined 8 d after ethanol-injection. Ethanol-injection induced sufficient pressure from ambrosia beetles to evaluate the efficacy of insecticides for preventing attacks. Trunk sprays of permethrin suppressed cumulative total attacks by ambrosia beetles in most tests. Trunk sprays of the botanical formulations Armorex and Veggie Pharm suppressed cumulative total attacks in Ohio. Armorex, Armorex + Permethrin, and Veggie Pharm + Permethrin suppressed attacks in Tennessee. The bifenthrin product Onyx suppressed establishment of X. germanus in one Ohio experiment, and cumulative total ambrosia beetle attacks in Virginia. Substrate drenches and trunk sprays of neonicotinoids, or trunk sprays of anthranilic diamides or tolfenpyrad were not effective. Ethanol-injection is effective for inducing attacks and ensuring pressure by ambrosia beetles for testing insecticide efficacy on ornamental trees. C1 [Reding, Michael E.; Ranger, Christopher M.] ARS, Hort Insects Res Grp, USDA, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Oliver, Jason B.; Youssef, Nadeer N.] Tennessee State Univ, Otis L Floyd Nursery Res Ctr, Coll Agr Human & Nat Sci, Mcminnville, TN 37110 USA. [Schultz, Peter B.] Virginia Tech Univ, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA 23455 USA. RP Reding, ME (reprint author), ARS, Hort Insects Res Grp, USDA, Ohio Agr Res & Dev Ctr, 1680 Madison Ave, Wooster, OH 44691 USA. EM mike.reding@ars.usda.gov FU USDA-ARS Floriculture and Nursery Research Initiative; ARS [3607-22000-012-00D] FX We thank Betsy Anderson (USDA-Agriculture Research Service) and Alicia Bray (Tennessee State University) for their assistance with ambrosia beetle identifications, and Jim Moyseenko (USDA-ARS), Joshua Basham, Joseph Lampley, Samuel Patton, Lacey Zarifan (Tennessee State University), Carmella Whitaker (Virginia Tech University), and Jacob Weddington and Victoria Eschenbacher (Warren County, TN High School Interns) for their technical assistance. This research was supported by funding from the USDA-ARS Floriculture and Nursery Research Initiative and base funds associated with ARS research project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine). NR 33 TC 11 Z9 12 U1 0 U2 26 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 289 EP 298 DI 10.1603/EC12315 PG 10 WC Entomology SC Entomology GA 089BC UT WOS:000314882200036 PM 23448043 ER PT J AU Jenkins, DA Cline, AR Irish, B Goenaga, R AF Jenkins, David A. Cline, Andrew R. Irish, Brian Goenaga, Ricardo TI Attraction of Pollinators to Atemoya (Magnoliales: Annonaceae) in Puerto Rico: A Synergistic Approach Using Multiple Nitidulid Lures SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Europs; Loberus testaceus; Omatomidae; Erotylidae; Nitidulidae ID STONE-FRUIT-ORCHARDS; AGGREGATION PHEROMONES; BEETLES COLEOPTERA; SAP BEETLES; CARPOPHILUS; COATTRACTANT; PHENOLOGY; EFFICACY; LUGUBRIS; FLORIDA AB Atemoya, a hybrid between Annona squamosa (L.) and A. cherimola Miller (Annonaceae), has potential to be a major fruit crop in tropical and subtropical areas. A major setback to fruit production throughout the world is low fruit-set because of inadequate pollinator visits, typically Nitidulidae beetles. We identified beetle visitors to atemoya flowers in an orchard in Puerto Rico and used Universal moth traps to monitor the attractiveness of two commercially available Nitidulidae lures. The most common visitors to atemoya flowers were an unidentified Europs species (Coleoptera: Monotomidae), followed by Loberus testaceus (Coleoptera: Erotylidae), neither of which have been previously reported as visitors to Annona flowers. The commercial lures attracted few or no beetles when used separately, but attracted a large number of beetles, especially Carpophilus dimidiatus (Coleoptera: Nitidulidae) and Europs, when used in combination. This attraction is synergistic and increases with dose at the doses assayed (0-4 lures), and decreases over time with >50% of trap captures occurring in the first week and no beetles collected after 5 wk. This is the first report of aggregation pheromone lures in nitidulids acting synergistically to attract other species, including beetles not in the Nitidulidae. The results are discussed as they pertain to increasing fruit set, as well as the potential for altering fruit size and shape in atemoya. C1 [Jenkins, David A.; Irish, Brian; Goenaga, Ricardo] ARS, USDA, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Cline, Andrew R.] Calif Dept Food & Agr, Plant Hlth Serv, Sacramento, CA 95814 USA. [Cline, Andrew R.] Calif Dept Food & Agr, Pest Prevent Serv, Sacramento, CA 95814 USA. RP Jenkins, DA (reprint author), ARS, USDA, Trop Agr Res Stn, 2200 Ave PA Campos,Ste 201, Mayaguez, PR 00680 USA. EM david.jenkins@ars.usda.gov NR 33 TC 2 Z9 3 U1 2 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 305 EP 310 DI 10.1603/EC12316 PG 6 WC Entomology SC Entomology GA 089BC UT WOS:000314882200038 PM 23448045 ER PT J AU Edwards, KT Caprio, MA Allen, KC Musser, FR AF Edwards, Kristine T. Caprio, Michael A. Allen, K. Clint Musser, Fred R. TI Risk Assessment for Helicoverpa zea (Lepidoptera: Noctuidae) Resistance on Dual-Gene Versus Single-Gene Corn SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE population genetic; insect; stochastic processes ID HELIOTHIS-VIRESCENS LEPIDOPTERA; INSECT RESISTANCE; BT CROPS; TRANSGENIC COTTON; HOST USE; MANAGEMENT; TOXIN; POPULATIONS; EVOLUTION; REFUGES AB Recent Environmental Protection Agency (EPA) decisions regarding resistance management in Bt-cropping systems have prompted concern in some experts that dual-gene Bt-corn (Cry1A.105 and Cry2Ab2 toxins) may result in more rapid selection for resistance in Helicoverpa zea (Boddie) than single-gene Bacillus thuringiensis (Bt)-corn (Cry1Ab toxin). The concern is that Bt-toxin longevity could be significantly reduced with recent adoption of a natural refuge for dual-gene Bt-cotton (Cry1Ac and Cry2Ab2 toxins) and concurrent reduction in dual-gene corn refuge from 50 to 20%. A population genetics framework that simulates complex landscapes was applied to risk assessment. Expert opinions on effectiveness of several transgenic corn and cotton varieties were captured and used to assign probabilities to different scenarios in the assessment. At least 350 replicate simulations with randomly drawn parameters were completed for each of four risk assessments. Resistance evolved within 30 yr in 22.5% of simulations with single-gene corn and cotton with no volunteer corn. When volunteer corn was added to this assessment, risk of resistance evolving within 30 yr declined to 13.8%. When dual-gene Bt-cotton planted with a natural refuge and single-gene corn planted with a 50% structured refuge was simulated, simultaneous resistance to both toxins never occurred within 30 yr, but in 38.5% of simulations, resistance evolved to toxin present in single-gene Bt-corn (Cry1Ab). When both corn and cotton were simulated as dual-gene products, cotton with a natural refuge and corn with a 20% refuge, 3% of simulations evolved resistance to both toxins simultaneously within 30 yr, while 10.4% of simulations evolved resistance to Cry1Ab/c toxin. C1 [Edwards, Kristine T.; Caprio, Michael A.; Musser, Fred R.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Miss State, MS 39762 USA. [Allen, K. Clint] ARS, USDA, So Insect Management Res Unit, Stoneville, MS 38776 USA. RP Edwards, KT (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Box 9775, Miss State, MS 39762 USA. EM kt20@msstate.edu FU USDA-ARS-SCA [58-6402-8-274] FX Funding for this research was provided by USDA-ARS-SCA Grant 58-6402-8-274. We are grateful to Omath-thage Perera and Ryan Jackson for helpful comments on the manuscript. This manuscript has been approved for publication as Journal Article No. J-12161 of the Mississippi Agriculture and Forestry Experiment Station, Mississippi State University. NR 36 TC 8 Z9 8 U1 0 U2 33 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 382 EP 392 DI 10.1603/EC12203 PG 11 WC Entomology SC Entomology GA 089BC UT WOS:000314882200048 PM 23448055 ER PT J AU Fuentes-Contreras, E Figueroa, CC Silva, AX Bacigalupe, LD Briones, LM Foster, SP Unruh, TR AF Fuentes-Contreras, Eduardo Figueroa, Christian C. Silva, Andrea X. Bacigalupe, Leonardo D. Briones, Lucia M. Foster, Stephen P. Unruh, Thomas R. TI Survey of Resistance to Four Insecticides and their Associated Mechanisms in Different Genotypes of the Green Peach Aphid (Hemiptera: Aphididae) From Chile SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Myzus persicae; cyfluthrin; metamidophos; pirimicarb; imidacloprid ID MYZUS-PERSICAE HEMIPTERA; FITNESS TRADE-OFF; POTATO APHID; NEONICOTINOID INSECTICIDES; PYRETHROID INSECTICIDES; REPRODUCING POPULATIONS; KNOCKDOWN RESISTANCE; HOMOPTERA-APHIDIDAE; SULZER HEMIPTERA; SOUTHERN FRANCE AB The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae) is a major pest of agriculture worldwide that has proved to be particularly adept at evolving insecticide resistance. Several mechanisms that confer resistance to many insecticide types have been described in M. persicae. We measured the resistance status of nine multilocus genotypes (MLGs) of this aphid species collected in Chile. MLGs were identified using microsatellite markers, and these MLG clonal populations were measured for the presence of modified acetylcholinesterase (MACE), kdr and super kdr mutations, and enhanced carboxyl esterase activity. Toxicological bioassays were used to estimate aphid LC50 when treated with metamidophos (organophosphate), pirimicarb (dimethyl carbamate), cyfluthrin (pyrethroid), and imidacloprid (neonicotinoid). Two MLGs presented >20-fold resistance to pirimicarb, which was associated with the MACE mutation in the heterozygous condition. The kdr mutation was found in only four MLGs in the heterozygous condition and they showed resistance ratios (RR) to cyfluthrin of less than sevenfold. The super kdr mutation was not detected. Enhanced carboxyl esterase activity was predominantly found in the susceptible (S) to first level of resistance (R-1) with RR to metamidophos less than eight-fold. Finally, RR to imidacloprid was also less than eight-fold in all MLGs tested. A few MLGs with resistance to pirimicarb were found, while susceptibility to cyfluthrin, metamidophos and imidacloprid was still predominant. A significant positive correlation between imidacloprid tolerance with pirimicarb resistance was detected, as well as between imidacloprid and metamidophos tolerance. With the increase in the use of neonicotinoid insecticides, better rotation of insecticides with different modes of action will be necessary to prevent further development of M. persicae insecticide resistance in Chile. C1 [Fuentes-Contreras, Eduardo] Univ Talca, Fac Ciencias Agr, Dept Prod Agr, Talca, Chile. [Figueroa, Christian C.; Silva, Andrea X.; Bacigalupe, Leonardo D.; Briones, Lucia M.] Univ Austral Chile, Fac Ciencias, Inst Ciencias Ambientales & Evolut, Valdivia, Chile. [Foster, Stephen P.] Rothamsted Res, Harpenden AL5 2JQ, Herts, England. [Unruh, Thomas R.] ARS, USDA, Yakima, WA 98951 USA. RP Fuentes-Contreras, E (reprint author), Univ Talca, Fac Ciencias Agr, Dept Prod Agr, Casilla 747, Talca, Chile. EM efuentes@utalca.cl RI Fuentes-Contreras, Eduardo/A-7595-2008; Figueroa, Christian/A-1170-2008 OI Fuentes-Contreras, Eduardo/0000-0003-3567-3830; Figueroa, Christian/0000-0001-9218-5564 FU Fondecyt [1090378, 1080085] FX Technical support form Carlos Cavieres is greatly acknowledged. This work was funded by Fondecyt 1090378 to C.C.F. and E.F.C. and Fondecyt 1080085 to L.D.B. and E.F.C. NR 61 TC 7 Z9 7 U1 3 U2 70 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 400 EP 407 DI 10.1603/EC12176 PG 8 WC Entomology SC Entomology GA 089BC UT WOS:000314882200050 PM 23448057 ER PT J AU Barr, N Ruiz-Arce, R Obregon, O De Leon, R Foster, N Reuter, C Boratynski, T Vacek, D AF Barr, Norman Ruiz-Arce, Raul Obregon, Oscar De Leon, Rosita Foster, Nelson Reuter, Chris Boratynski, Theodore Vacek, Don TI Molecular Diagnosis of Populational Variants of Anthonomus grandis (Coleoptera: Curculionidae) in North America SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE boll weevil; DNA barcode; COI DNA; host use; cotton pest ID BOLL-WEEVILS COLEOPTERA; MITOCHONDRIAL-DNA; GENETIC-VARIATION; ERADICATION ZONE; IDENTIFICATION; PRIMERS; DIPTERA; TEPHRITIDAE; ASSIGNMENT; DIVERSITY AB The utility of the cytochrome oxidase I (COI) DNA sequence used for DNA barcoding and a Sequence Characterized Amplified Region for diagnosing boll weevil, Anthonomus grandis Boheman, variants was evaluated. Maximum likelihood analysis of COI DNA sequences from 154 weevils collected from the United States and Mexico supports previous evidence for limited gene flow between weevil populations on wild cotton and commercial cotton in northern Mexico and southern United States. The wild cotton populations represent a variant of the species called the thurberia weevil, which is not regarded as a significant pest. The 31 boll weevil COI haplotypes observed in the study form two distinct haplogroups (A and B) that are supported by five fixed nucleotide differences and a phylogenetic analysis. Although wild and commercial cotton populations are closely associated with specific haplogroups, there is not a fixed difference between the thurberia weevil variant and other populations. The Sequence Characterized Amplified Region marker generated a larger number of inconclusive results than the COI gene but also supported evidence of shared genotypes between wild and commercial cotton weevil populations. These methods provide additional markers that can assist in the identification of pest weevil populations but not definitively diagnose samples. C1 [Barr, Norman; Ruiz-Arce, Raul; Obregon, Oscar; De Leon, Rosita; Vacek, Don] USDA APHIS, Ctr Plant Hlth Sci & Technol, Mission Lab, Moore Air Base, Edinburg, TX 78541 USA. [Foster, Nelson; Reuter, Chris] USDA APHIS, Ctr Plant Hlth Sci & Technol, Phoenix Lab, Phoenix, AZ 85040 USA. [Boratynski, Theodore] USDA APHIS, Brawley, CA 92227 USA. RP Barr, N (reprint author), USDA APHIS, Ctr Plant Hlth Sci & Technol, Mission Lab, Moore Air Base, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM mexfly@gmail.com NR 32 TC 0 Z9 0 U1 1 U2 11 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 437 EP 449 DI 10.1603/EC12340 PG 13 WC Entomology SC Entomology GA 089BC UT WOS:000314882200054 PM 23448061 ER PT J AU Buckman, KA Campbell, JF Subramanyam, B AF Buckman, Karrie A. Campbell, James F. Subramanyam, Bhadriraju TI Tribolium castaneum (Coleoptera: Tenebrionidae) Associated With Rice Mills: Fumigation Efficacy and Population Rebound SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE red flour beetle; fumigation; sulfuryl fluoride; pheromone trap; monitoring ID FLOUR MILLS; HERBST COLEOPTERA; STORED-PRODUCT; WAREHOUSES; INSECTS; IMPACT AB Thered flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is the most important stored-product insect pest infesting rice (Oryza sativa L.) mills in the United States. Due to the phasing out of methyl bromide in accordance with the 1987 Montreal Protocol, the efficacy of alternative fumigants in controlling flour beetles in mill structures must be evaluated. Long-term trapping data sets (2-6 yr) of T. castaneum in and around seven rice mills were analyzed to assess the efficacy of sulfuryl fluoride fumigation (n = 25). Fumigation efficacy was evaluated as the percentage reduction in mean trap captures of adults and proportion of traps capturing at least one adult beetle. Beetle trap captures fluctuated seasonally, with increased captures during the warmer months, June-September, that dropped off during the cooler months, October-March. Fumigations resulted in a 66 +/- 6% (mean +/- SE) reduction in mean trap captures within mills and a 52 +/- 6% reduction in the proportion of traps capturing at least one adult beetle. Lengths of time for captures to reach prefumigation levels, or rebound rates, were variable, and adult capture levels inside were most influenced by seasonal temperature changes. Temperatures inside mills followed those outside the mill closely, and a significant positive relationship between outside temperatures and trap captures was observed. Inside and outside trap captures exhibited a significant, positive relationship, but fumigations consistently led to reductions in beetle captures outside of mills, highlighting the interconnectedness of populations located inside and outside mill structures. C1 [Buckman, Karrie A.; Campbell, James F.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RP Buckman, KA (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM karrie.buckman@ars.usda.gov FU USDA RAMP [2007-51101-18407]; USDA Methyl Bromide Alternative [2011-51102-31125] FX We thank the management at the rice mills for allowing access to facilities and for cooperation in trap shipment. We are grateful to R. Hammel for technical support with the assistance of J. Ediger, Z. Goldstein, M. Hartzer, C. Persson, D. Robbins, J. Staats, and M. Van Tuyl. We thank three anonymous reviewers for helpful comments on an earlier draft. This study was supported in part by USDA RAMP (agreement 2007-51101-18407) and USDA Methyl Bromide Alternative (agreement 2011-51102-31125) grants. This article is contribution 13-013-J from the Kansas Agricultural Experiment Station. NR 23 TC 10 Z9 10 U1 0 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 499 EP 512 DI 10.1603/EC12276 PG 14 WC Entomology SC Entomology GA 089BC UT WOS:000314882200060 PM 23448067 ER PT J AU Neven, LG AF Neven, Lisa G. TI Effects of Short Photoperiod on Codling Moth Diapause and Survival SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE auarantine; export; apples; diapause AB The potential presence of codling moth, Cydia pomonella L., in apples shipped to countries within the 30th latitudes has raised concerns that this pest could establish and spread in these countries. Previous research demonstrated that codling moth in apples handled under simulated commercial cold storage conditions and held under short day lengths could not break diapause and emerge in sufficient numbers to establish a minimum viable population. This study expands the in-fruit work by examining the ability of codling moth to establish a laboratory population under a short photoperiod of 12: 12 ( L: D) h, as compared with a long photoperiod of 16: 8 ( L: D) h. Codling moth larvae were collected from field infested fruits in 2010 and 2011. Moths were collected from the infested fruits and separated into two groups representing the two daylength conditions. In total, 1,004 larvae were monitored for adult emergence and ability to generate a subsequent population. Larvae held under the photoperiod of 12: 12 ( L: D) h generated only one moth in the 2 yr period, whereas larvae held under the photoperiod of 16: 8 ( L: D) h generated 186 females and 179 males, that sustained subsequent generations on artificial diet under laboratory conditions. These results indicate that under controlled environmental conditions, codling moth cannot complete diapause and emerge in sufficient numbers to sustain a viable population when held under a short photoperiod. C1 ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Neven, LG (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM lisa.neven@ars.usda.gov FU Washington Tree Fruit Research Commission FX This research was made possible through a grant from the Washington Tree Fruit Research Commission. I acknowledge the exemplary efforts of Anne Kenny Chapman, USDA-Agriculture Research Service (ARS), in the design of the experiments, conducting the tests, and summarizing and preliminary analysis of the data as well as writing the materials and methods section. I would like to thank Michele Watkins and Fred Lopez, USDA-ARS, for their technical assistance. We would also like to thank Wee Yee and Sunil Kumar for peer reviews. NR 9 TC 3 Z9 3 U1 2 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2013 VL 106 IS 1 BP 520 EP 523 DI 10.1603/EC12366 PG 4 WC Entomology SC Entomology GA 089BC UT WOS:000314882200062 PM 23448069 ER PT J AU Buuveibaatar, B Young, JK Berger, J Fine, AE Lkhagvasuren, B Zahler, P Fuller, TK AF Buuveibaatar, Bayarbaatar Young, Julie K. Berger, Joel Fine, Amanda E. Lkhagvasuren, Badamjav Zahler, Peter Fuller, Todd K. TI Factors affecting survival and cause-specific mortality of saiga calves in Mongolia SO JOURNAL OF MAMMALOGY LA English DT Article DE birth mass; litter size; Saiga tatarica mongolica; season; sex; twinning rates ID WHITE-TAILED DEER; POPULATION-DYNAMICS; TATARICA-MONGOLICA; TEMPORAL VARIATION; LARGE HERBIVORES; PLANT PHENOLOGY; BIGHORN LAMBS; CENTRAL-ASIA; HABITAT USE; MULE DEER AB Factors affecting juvenile survival are poorly known in the world's most northern antelope, the endangered saiga (Saiga tatarica), yet these factors are fundamental for understanding what drives population change. We monitored Mongolia saiga (S. tatarica mongolica) calves in Sharga Nature Reserve, western Mongolia, during 2008-2010. Our results showed that male and single calves were heavier than females and twins, respectively. However, we identified no significant differences in seasonal or annual survival rates between sexes or between singletons and twins. Litter size and birth mass varied among years, and there was a negative relationship between these variables. Survival of calves during the 1st year was best explained by the covariates of year and litter size (confounded with body mass), suggesting that interannual variation in environmental conditions influenced twinning rates and body mass, and might play a key role in 1st-year survival. We identified 3 sources of mortality predation by raptors, foxes (red fox [Vulpes vulpes] and corsac fox [V. corsac]), and lynx (Lynx lynx). Most predation was attributed to raptors, such as golden eagles (Aquila chrysaetos) and cinereous vultures (Aegypius monachus). Our results point to both environmental and biotic factors affecting survival of juvenile saiga. C1 [Buuveibaatar, Bayarbaatar; Fine, Amanda E.] Wildlife Conservat Soc, Mongolia Program, Ulaanbaatar, Mongol Peo Rep. [Buuveibaatar, Bayarbaatar; Lkhagvasuren, Badamjav] Mongolian Acad Sci, Inst Biol, Ulaanbaatar 51, Mongol Peo Rep. [Buuveibaatar, Bayarbaatar; Fuller, Todd K.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Young, Julie K.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Logan, UT 84322 USA. [Young, Julie K.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Berger, Joel] Univ Montana, No Rockies Field Off, Wildlife Conservat Soc, Missoula, MT 59812 USA. [Berger, Joel] Univ Montana, Missoula, MT 59812 USA. [Berger, Joel] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Lkhagvasuren, Badamjav] World Wide Fund Nat, Mongolia Program Off, Ulaanbaatar, Mongol Peo Rep. [Zahler, Peter] Wildlife Conservat Soc, Asia Program, Bronx, NY 10460 USA. RP Buuveibaatar, B (reprint author), Wildlife Conservat Soc, Mongolia Program, Amar Str 3,Internom Bookstore Bldg,3rd Floor, Ulaanbaatar, Mongol Peo Rep. EM bbayarba@eco.umass.edu FU Wildlife Conservation Society; National Geographic Society; Rufford Small Grants; Zoological Society of London's EDGE Fellowship Program; Institute for Wildlife Studies FX This research was funded by the Wildlife Conservation Society, National Geographic Society, Rufford Small Grants, Zoological Society of London's EDGE Fellowship Program, and Institute for Wildlife Studies. We thank Ts. Buyanbat, WWF Mongolia saiga ranger, for monitoring the marked animals and all of the research assistants who helped during the calving seasons. The Wildlife Conservation Society's Mongolia Program Office, Institute of Biology, Mongolian Academy of Sciences, and Ministry of Nature, Environment, and Tourism of Mongolia facilitated this research. Improvements to the manuscript were contributed by 3 anonymous reviewers. NR 76 TC 5 Z9 5 U1 3 U2 48 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 J9 J MAMMAL JI J. Mammal. PD FEB PY 2013 VL 94 IS 1 BP 127 EP 136 DI 10.1644/11-MAMM-A-077.1 PG 10 WC Zoology SC Zoology GA 095WY UT WOS:000315366900013 ER PT J AU Li, EC Lim, C Klesius, PH Welker, TL AF Li, Erchao Lim, Chhorn Klesius, Phillip H. Welker, Thomas L. TI Growth, Body Fatty Acid Composition, Immune Response, and Resistance to Streptococcus iniae of Hybrid Tilapia, Oreochromis niloticusxOreochromis aureus, Fed Diets Containing Various Levels of Linoleic and Linolenic Acids SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID ALTERNATIVE COMPLEMENT PATHWAY; CATFISH ICTALURUS-PUNCTATUS; BREAM SPARUS-AURATA; COD-LIVER OIL; CHANNEL CATFISH; LIPID SOURCE; ALPHA-TOCOPHEROL; FLAXSEED OIL; NILOTICUS; REQUIREMENTS AB The effects of dietary linoleic (LA) and linolenic acids (LN) on growth and immunity of all-male hybrid tilapia, Oreochromis niloticusxOreochromis aureus, were evaluated for 10wk. Fish fed 0.12% LA+ 0% LN had the lowest weight gain (WG) but was not significantly different from diets containing 0.5% LA or 0.40% LA+ 1.0% LN. Fish fed 1% LA had the highest WG but did not differ from diets with 0.5% LA, 2.0% LA, 0.26% LA+ 0.5% LN, 0.69% LA+ 2.0% LN, or diets containing both LA and LN at 0.25, 0.5, and 1.0%. Feed intake, feed efficiency, and survival did not differ among treatments. Total body n-6 fatty acids (FAs) increased with increasing dietary levels of n-6. Total body n-3 FAs also appeared to increase with increasing dietary n-3 levels but peaked at 1% of diet. Dietary treatment had no effect on hematology, immune function, or survival to Streptococcus iniae. This study indicates that both LA and LN are dietary essential for growth of hybrid tilapia. Dietary LA alone can meet the essential FA requirement, and a level of 1.14% of diet is required for optimum growth. C1 [Li, Erchao] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China. [Lim, Chhorn; Klesius, Phillip H.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. [Welker, Thomas L.] USDA ARS, Hagerman Fish Culture Stn, Hagerman, ID USA. RP Lim, C (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. NR 54 TC 10 Z9 14 U1 2 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD FEB PY 2013 VL 44 IS 1 BP 42 EP 55 DI 10.1111/jwas.12014 PG 14 WC Fisheries SC Fisheries GA 090IV UT WOS:000314973600003 ER PT J AU Chatakondi, NG Kelly, AM AF Chatakondi, Nagaraj G. Kelly, Anita M. TI Oocyte Diameter and Plasma Vitellogenin as Predictive Factors to Identify Potential Channel Catfish, Ictalurus punctatus, Suitable for Induced Spawning SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID SALMON SALMO-SALAR; BLUE CATFISH; MORPHOPHYSIOLOGICAL PREDICTORS; REPRODUCTIVE-CYCLE; OVULATORY SUCCESS; WHITE STURGEON; HORMONE ANALOG; CHANOS-CHANOS; GROWTH; MATURATION AB This study monitors the progression of oocyte size and plasma hormone profiles of female channel catfish, Ictalurus punctatus, at monthly intervals to stage expectant ovulating, females for strip spawning. The critical minimum diameter of an oocyte to reach threshold maturity for channel catfish was 2.5 +/- 0.21mm. Monthly increases of oocyte diameters and plasma vitellogenin concentrations were linear until spawning. The reproductive performance of cannulated and noncannulated catfish did not differ, negating adverse effects of routine cannulation. This study suggests oocyte diameter in channel catfish can be used as a predictive factor to determine and stage potential broodfish suitable for hormone-induced spawning. C1 [Chatakondi, Nagaraj G.] Harvest Select Farms, Inverness, MS 38753 USA. [Kelly, Anita M.] So Illinois Univ, Fisheries & Illinois Aquaculture Ctr, Carbondale, IL 62901 USA. RP Chatakondi, NG (reprint author), USDA ARS, Catfish Genet Res Unit, Stoneville, MS 38776 USA. FU USDA SBIR [04088] FX We are grateful to Roger Yant and the staff of Harvest Select Farms, Inverness, Mississippi, for assistance with this research project. This work constituted a part of the research project funded by USDA SBIR phase II - 04088. Mention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the U.S. Department of Agriculture and does not imply approval to the exclusion of other products that may be suitable. NR 46 TC 0 Z9 1 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD FEB PY 2013 VL 44 IS 1 BP 115 EP 123 DI 10.1111/jwas.12001 PG 9 WC Fisheries SC Fisheries GA 090IV UT WOS:000314973600010 ER PT J AU Zhong, SL Fei, ZJ Chen, YR Zheng, Y Huang, MY Vrebalov, J McQuinn, R Gapper, N Liu, B Xiang, J Shao, Y Giovannoni, JJ AF Zhong, Silin Fei, Zhangjun Chen, Yun-Ru Zheng, Yi Huang, Mingyun Vrebalov, Julia McQuinn, Ryan Gapper, Nigel Liu, Bao Xiang, Jenny Shao, Ying Giovannoni, James J. TI Single-base resolution methylomes of tomato fruit development reveal epigenome modifications associated with ripening SO NATURE BIOTECHNOLOGY LA English DT Article ID EPIGENETIC MUTATION; REGULATORY REGIONS; GENE-EXPRESSION; DNA; ETHYLENE; METHYLATION; TRANSCRIPTION; ARABIDOPSIS; GENOME; PLANTS AB Ripening of tomato fruits is triggered by the plant hormone ethylene, but its effect is restricted by an unknown developmental cue to mature fruits containing viable seeds. To determine whether this cue involves epigenetic remodeling, we expose tomatoes to the methyltransferase inhibitor 5-azacytidine and find that they ripen prematurely. We performed whole-genome bisulfite sequencing on fruit in four stages of development, from immature to ripe. We identified 52,095 differentially methylated regions (representing 1% of the genome) in the 90% of the genome covered by our analysis. Furthermore, binding sites for RIN, one of the main ripening transcription factors, are frequently localized in the demethylated regions of the promoters of numerous ripening genes, and binding occurs in concert with demethylation. Our data show that the epigenome is not static during development and may have been selected to ensure the fidelity of developmental processes such as ripening. Crop-improvement strategies could benefit by taking into account not only DNA sequence variation among plant lines, but also the information encoded in the epigenome. C1 [Zhong, Silin; Fei, Zhangjun; Chen, Yun-Ru; Zheng, Yi; Huang, Mingyun; Vrebalov, Julia; McQuinn, Ryan; Gapper, Nigel; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Zhong, Silin; Liu, Bao] NE Normal Univ, Minist Educ, Key Labs Mol Epigenet, Changchun, Peoples R China. [Fei, Zhangjun; Giovannoni, James J.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Xiang, Jenny; Shao, Ying] Cornell Univ, Weill Med Coll, Genom Resource Core Facil, New York, NY 10021 USA. RP Giovannoni, JJ (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM sz284@cornell.edu; zf25@cornell.edu; jjg33@cornell.edu RI zhong, silin/O-7029-2015; Zheng, Yi/F-6150-2016; OI zhong, silin/0000-0002-0198-7383; Zheng, Yi/0000-0002-8042-7770; Fei, Zhangjun/0000-0001-9684-1450 FU United States Department of Agriculture - Agricultural Research Service, National Science Foundation [IOS-0606595, IOS-0923312, DBI-0820612]; National Natural Science Foundation of China [30900783, 3090243]; Human Frontier Science Program [LTF000076/2009] FX We thank E. Richards, R. Schmitz and J. Ecker for discussion and thoughtful advice in preparing this manuscript, and R. White, Y. Xu and Z. Li for technical assistance. This project was supported by the United States Department of Agriculture - Agricultural Research Service, National Science Foundation IOS-0606595 and IOS-0923312 to J.J.G. and Z.F., DBI-0820612 to J.J.G., National Natural Science Foundation of China 30900783 and 3090243 to S.Z. and B.L. and the Human Frontier Science Program LTF000076/2009 to S.Z. NR 30 TC 147 Z9 155 U1 19 U2 151 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1087-0156 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD FEB PY 2013 VL 31 IS 2 BP 154 EP 159 DI 10.1038/nbt.2462 PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 095GG UT WOS:000315322100027 PM 23354102 ER PT J AU Muhammad, K Afghan, S Pan, YB Iqbal, J AF Muhammad, Khushi Afghan, Shahid Pan, Yong-Bao Iqbal, Javed TI GENETIC VARIABILITY AMONG THE BROWN RUST RESISTANT AND SUSCEPTIBLE GENOTYPES OF SUGARCANE BY RAPD TECHNIQUE SO PAKISTAN JOURNAL OF BOTANY LA English DT Article ID DNA POLYMORPHISMS; SACCHARUM SPP.; MARKERS; GENOME; DIVERSITY; CULTIVARS; CLONES; PCR; L. AB Brown leaf rust in sugarcane is caused by Puccinia melanocephala (Syd. & P. Syd.), which is a major cause of cultivar withdrawal. We attempted to analyze the RAPD diversity of two discrete phenotypic classes, i.e., rust resistant (R) and rust susceptible (S) of six commercially available sugarcane elite genotypes in Pakistan. DNA samples were extracted from these cultivars. The genomic DNA was amplified with 160 decamer primers and 33 of them generated 216 trackable loci with the range of 150-2500 bp. The total polymorphic bands were 164 (76%) and these were used to cluster rust resistant and susceptible cultivars. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) based on Nei's genetic distance was analyzed by NTSYSpc and showed that 6 genotypes were clustered into 3 groups with the range from 63% to 73% genetic distances. This observation indicated the significant correlation between genetic distance and disease. Ten of 33 decamers (30%) had shown 100% polymorphism with three polymorphic bands/loci, which were generated in two different discrete groups rust resistant and rust susceptible. In this study we have found 17 cultivars specific loci generated by 11 RAPD primers and cluster analysis revealed three main groups and two subgroups against rust susceptible genotypes to develop characterization of genotypes at molecular level. Additionally, this explored the genetic basis of discrete phenotypic classes in sugarcane genome for marker development. C1 [Muhammad, Khushi; Iqbal, Javed] Univ Punjab, Sch Biol Sci, Lahore, Pakistan. [Afghan, Shahid] Shakarganj Sugarcane Res Inst, Jhang, Pakistan. [Pan, Yong-Bao] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. RP Muhammad, K (reprint author), Univ Punjab, Sch Biol Sci, Lahore, Pakistan. EM khushisbs@yahoo.com RI Iqbal, Javed/H-9604-2015 NR 38 TC 1 Z9 1 U1 0 U2 5 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD FEB PY 2013 VL 45 IS 1 BP 163 EP 168 PG 6 WC Plant Sciences SC Plant Sciences GA 094MN UT WOS:000315268000022 ER PT J AU Cooper, L Walls, RL Elser, J Gandolfo, MA Stevenson, DW Smith, B Preece, J Athreya, B Mungall, CJ Rensing, S Hiss, M Lang, D Reski, R Berardini, TZ Li, DH Huala, E Schaeffer, M Menda, N Arnaud, E Shrestha, R Yamazaki, Y Jaiswal, P AF Cooper, Laurel Walls, Ramona L. Elser, Justin Gandolfo, Maria A. Stevenson, Dennis W. Smith, Barry Preece, Justin Athreya, Balaji Mungall, Christopher J. Rensing, Stefan Hiss, Manuel Lang, Daniel Reski, Ralf Berardini, Tanya Z. Li, Donghui Huala, Eva Schaeffer, Mary Menda, Naama Arnaud, Elizabeth Shrestha, Rosemary Yamazaki, Yukiko Jaiswal, Pankaj TI The Plant Ontology as a Tool for Comparative Plant Anatomy and Genomic Analyses SO PLANT AND CELL PHYSIOLOGY LA English DT Article DE Bioinformatics; Comparative genomics; Genome annotation; Ontology; Plant anatomy; Terpene synthase ID MOSS PHYSCOMITRELLA-PATENS; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; INTEGRATED INFORMATION; PHYLOGENETIC TREES; TERPENE SYNTHASES; INTERACTIVE TREE; FLOWER FORMATION; DRAFT SEQUENCE; WEB SERVICES AB The Plant Ontology (PO; ext-link-type="uri" xlink:href="http://www.plantontology.org/" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.plantontology.org/) is a publicly available, collaborative effort to develop and maintain a controlled, structured vocabulary ('ontology') of terms to describe plant anatomy, morphology and the stages of plant development. The goals of the PO are to link (annotate) gene expression and phenotype data to plant structures and stages of plant development, using the data model adopted by the Gene Ontology. From its original design covering only rice, maize and Arabidopsis, the scope of the PO has been expanded to include all green plants. The PO was the first multispecies anatomy ontology developed for the annotation of genes and phenotypes. Also, to our knowledge, it was one of the first biological ontologies that provides translations (via synonyms) in non-English languages such as Japanese and Spanish. As of Release #18 (July 2012), there are about 2.2 million annotations linking PO terms to > 110,000 unique data objects representing genes or gene models, proteins, RNAs, germplasm and quantitative trait loci (QTLs) from 22 plant species. In this paper, we focus on the plant anatomical entity branch of the PO, describing the organizing principles, resources available to users and examples of how the PO is integrated into other plant genomics databases and web portals. We also provide two examples of comparative analyses, demonstrating how the ontology structure and PO-annotated data can be used to discover the patterns of expression of the LEAFY (LFY) and terpene synthase (TPS) gene homologs. C1 [Cooper, Laurel; Elser, Justin; Preece, Justin; Athreya, Balaji; Jaiswal, Pankaj] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Walls, Ramona L.; Stevenson, Dennis W.] New York Bot Garden, Bronx, NY 10458 USA. [Gandolfo, Maria A.] Cornell Univ, LH Bailey Hortorium, Dept Plant Biol, Ithaca, NY 14853 USA. [Smith, Barry] SUNY Buffalo, Dept Philosophy, Buffalo, NY 14260 USA. [Mungall, Christopher J.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley Bioinformat Open Source Projects, Berkeley, CA 94720 USA. [Rensing, Stefan; Hiss, Manuel; Lang, Daniel; Reski, Ralf] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany. [Rensing, Stefan; Hiss, Manuel] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, D-79104 Freiburg, Germany. [Reski, Ralf] Univ Freiburg, FRIAS Freiburg Inst Adv Studies, D-79104 Freiburg, Germany. [Berardini, Tanya Z.; Li, Donghui; Huala, Eva] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA. [Schaeffer, Mary] ARS, USDA, Columbia, MO 65211 USA. [Schaeffer, Mary] Univ Missouri, Div Plant Sci, Dept Agron, Columbia, MO 65211 USA. [Menda, Naama] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Arnaud, Elizabeth] Biovers Int, Rome, Italy. [Shrestha, Rosemary] CIMMYT, Genet Resources Program, Mexico City 06600, DF, Mexico. [Yamazaki, Yukiko] Natl Inst Genet, Ctr Genet Resource Informat, Mishima, Shizuoka 4118540, Japan. RP Jaiswal, P (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, 2082 Cordley Hall, Corvallis, OR 97331 USA. EM jaiswalp@science.oregonstate.edu RI Lang, Daniel/C-7238-2008; Jaiswal, Pankaj/H-7599-2016; OI Lang, Daniel/0000-0002-2166-0716; Jaiswal, Pankaj/0000-0002-1005-8383; Huala, Eva/0000-0003-4631-7241; Hiss, Manuel/0000-0002-7876-783X; Smith, Barry/0000-0003-1384-116X FU US National Science Foundation [IOS: 0822201] FX This work was supported by the US National Science Foundation [grant No. IOS: 0822201]. NR 104 TC 46 Z9 47 U1 2 U2 47 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-0781 J9 PLANT CELL PHYSIOL JI Plant Cell Physiol. PD FEB PY 2013 VL 54 IS 2 BP E1 EP + DI 10.1093/pcp/pcs163 PG 23 WC Plant Sciences; Cell Biology SC Plant Sciences; Cell Biology GA 093UR UT WOS:000315218700001 PM 23220694 ER PT J AU Shen, D Sun, HH Huang, MY Zheng, Y Li, XX Fei, ZJ AF Shen, Di Sun, Honghe Huang, Mingyun Zheng, Yi Li, Xixiang Fei, Zhangjun TI RadishBase: A Database for Genomics and Genetics of Radish SO PLANT AND CELL PHYSIOLOGY LA English DT Article DE radish; genomics; genetics; database ID RAPHANUS-SATIVUS L.; LINKAGE MAP; SSR MARKERS AB Radish is an economically important vegetable crop. During the past several years, large-scale genomics and genetics resources have been accumulated for this species. To store, query, analyze and integrate these radish resources efficiently, we have developed RadishBase ( ext-link-type="uri" xlink:href="http://bioinfo.bti.cornell.edu/radish" xmlns:xlink="http://www.w3.org/1999/xlink">http://bioinfo.bti.cornell.edu/radish), a genomics and genetics database of radish. Currently the database contains radish mitochondrial genome sequences, expressed sequence tag (EST) and unigene sequences and annotations, biochemical pathways, EST-derived single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers, and genetic maps. RadishBase is designed to enable users easily to retrieve and visualize biologically important information through a set of efficient query interfaces and analysis tools, including the BLAST search and unigene annotation query interfaces, and tools to classify unigenes functionally, to identify enriched gene ontology (GO) terms and to visualize genetic maps. A database containing radish pathways predicted from unigene sequences is also included in RadishBase. The tools and interfaces in RadishBase allow efficient mining of recently released and continually expanding large-scale radish genomics and genetics data sets, including the radish genome sequences and RNA-seq data sets. C1 [Shen, Di; Li, Xixiang] Chinese Acad Agr Sci, Key Lab Hort Crop Biol & Germplasm Innovat, Minist Agr, Inst Vegetables & Flowers, Beijing 100081, Peoples R China. [Shen, Di; Sun, Honghe; Huang, Mingyun; Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Sun, Honghe] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Fei, Zhangjun] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Fei, ZJ (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM zf25@cornell.edu RI Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU National Science Foundation [IOS-0923312]; National High-tech R&D Program of China [2012AA021801] FX This work was supported by National Science Foundation [IOS-0923312]; the National High-tech R&D Program of China [2012AA021801]. NR 16 TC 14 Z9 14 U1 0 U2 21 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-0781 J9 PLANT CELL PHYSIOL JI Plant Cell Physiol. PD FEB PY 2013 VL 54 IS 2 BP E3 EP + DI 10.1093/pcp/pcs176 PG 6 WC Plant Sciences; Cell Biology SC Plant Sciences; Cell Biology GA 093UR UT WOS:000315218700003 PM 23239846 ER PT J AU Zhang, CY Cahoon, RE Hunter, SC Chen, M Han, JX Cahoon, EB AF Zhang, Chunyu Cahoon, Rebecca E. Hunter, Sarah C. Chen, Ming Han, Jixiang Cahoon, Edgar B. TI Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production SO PLANT JOURNAL LA English DT Article DE vitaminE; tocopherol; tocotrienol; homogentisate; homogentisate geranylgeranyl transferase; homogentisate phytyltransferase ID ARABIDOPSIS HOMOGENTISATE PHYTYLTRANSFERASE; SYNECHOCYSTIS SP PCC-6803; AROMATIC-AMINO-ACIDS; 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE; TOCOPHEROL BIOSYNTHESIS; ISOPRENOID BIOSYNTHESIS; SEED TOCOPHEROL; PLANTS; EXPRESSION; PATHWAY AB VitaminE tocotrienol synthesis in monocots requires homogentisate geranylgeranyl transferase (HGGT), which catalyzes the condensation of homogentisate and the unsaturated C20 isoprenoid geranylgeranyl diphosphate (GGDP). By contrast, vitaminE tocopherol synthesis is mediated by homogentisate phytyltransferase (HPT), which condenses homogentisate and the saturated C20 isoprenoid phytyl diphosphate (PDP). An HGGT-independent pathway for tocotrienol synthesis has also been shown to occur by de-regulation of homogentisate synthesis. In this paper, the basis for this pathway and its impact on vitaminE production when combined with HGGT are explored. An Arabidopsis line was initially developed that accumulates tocotrienols and homogentisate by co-expression of Arabidopsis hydroxyphenylpyruvate dioxygenase (HPPD) and Escherichia coli bi-functional chorismate mutase/prephenate dehydrogenase (TyrA). When crossed into the vte21 HPT null mutant, tocotrienol production was lost, indicating that HPT catalyzes tocotrienol synthesis in HPPD/TyrA-expressing plants by atypical use of GGDP as a substrate. Consistent with this, recombinant Arabidopsis HPT preferentially catalyzed in vitro production of the tocotrienol precursor geranylgeranyl benzoquinol only when presented with high molar ratios of GGDP:PDP. In addition, tocotrienol levels were highest in early growth stages in HPPD/TyrA lines, but decreased strongly relative to tocopherols during later growth stages when PDP is known to accumulate. Collectively, these results indicate that HPPD/TyrA-induced tocotrienol production requires HPT and occurs upon enrichment of GGDP relative to PDP in prenyl diphosphate pools. Finally, combined expression of HPPD/TyrA and HGGT in Arabidopsis leaves and seeds resulted in large additive increases in vitaminE production, indicating that homogentisate concentrations limit HGGT-catalyzed tocotrienol synthesis. C1 [Zhang, Chunyu] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Zhang, Chunyu] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. [Zhang, Chunyu; Cahoon, Rebecca E.; Chen, Ming; Cahoon, Edgar B.] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA. [Zhang, Chunyu; Cahoon, Rebecca E.; Chen, Ming; Cahoon, Edgar B.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA. [Hunter, Sarah C.] Agr Res Serv, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. [Han, Jixiang] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. RP Cahoon, EB (reprint author), Univ Nebraska, Dept Biochem, 1901 Vine St, Lincoln, NE 68588 USA. EM ecahoon2@unl.edu FU US Department of Agriculture National Research Initiative [2004-35318-14887]; National Natural Science Foundation of China [31071453]; 111 Project [B07041] FX We thank Dean DellaPenna (Department of Biochemistry, Michigan State University, East Lansing, Michigan) for Arabidopsis vte2-1 seeds. This research was supported by a grant from the US Department of Agriculture National Research Initiative 2004-35318-14887. We also thank the National Natural Science Foundation of China (grant number 31071453) and the 111 Project (grant number B07041) for funding. We thank Tara Nazarenus for expert technical support. NR 47 TC 15 Z9 17 U1 4 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 J9 PLANT J JI Plant J. PD FEB PY 2013 VL 73 IS 4 BP 628 EP 639 DI 10.1111/tpj.12067 PG 12 WC Plant Sciences SC Plant Sciences GA 094WB UT WOS:000315294300009 PM 23137278 ER PT J AU Aradhya, M Wang, Y Walker, MA Prins, BH Koehmstedt, AM Velasco, D Gerrath, JM Dangl, GS Preece, JE AF Aradhya, M. Wang, Y. Walker, M. A. Prins, B. H. Koehmstedt, A. M. Velasco, D. Gerrath, J. M. Dangl, G. S. Preece, J. E. TI Genetic diversity, structure, and patterns of differentiation in the genus Vitis SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Vitis; Muscadinia; Genetic structure; Microsatellite; Phylogeny; Polymorphism; Taxonomy ID MULTILOCUS GENOTYPE DATA; AFLP DATA SETS; ANCESTRAL POLYMORPHISMS; PHYLOGENETIC ANALYSIS; RETICULATE EVOLUTION; DNA POLYMORPHISMS; WILD GRAPES; VITACEAE; MARKERS; LOCI AB Vitis (Vitaceae) is a taxonomically complicated genus with ca. 60 taxa divided into two subgenera, Vitis and Muscadinia. We used population genetic approaches to gain insights into the genetic diversity, patterns of evolutionary differentiation and to decipher the taxonomic status of some of the controversial taxa within the genus Vitis. The distance- and model-based analyses were used to examine the phylogenetic structure within the genus Vitis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. The results closely matched the current classification, but some discrepancies in the identity of taxa at the specific and subspecific levels were still evident. The East Asia and the North American Vitis exhibited strong divergence and each group showed further differentiation into several subgroups with North American subgroups roughly matching the described series. The model based cluster analysis indicated 14 clusters as optimum to explain the genetic structure within the genus Vitis with most clusters containing a moderate frequency of admixed genotypes suggesting interspecific gene flow within the subgenus Vitis. Hierarchical partitioning of molecular variation indicated that a significant amount of the total variation (similar to 74 % and similar to 69 % for SSRs and AFLPs, respectively) is accounted for by intraspecific variation as compared to the levels due to genetic differentiation among species within series (similar to 17 % and similar to 20 % for SSRs and AFLPs, respectively) and among series within the genus Vitis (similar to 9 % and similar to 10 % for SSRs and AFLPs, respectively). Overall, Vitis possesses mild genetic structure characterized by reticulation and incomplete lineage sorting of ancestral polymorphisms. C1 [Aradhya, M.; Prins, B. H.; Koehmstedt, A. M.; Preece, J. E.] USDA ARS Natl Clonal Germplasm Repository, Davis, CA 95616 USA. [Wang, Y.] NW A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [Walker, M. A.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Velasco, D.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Gerrath, J. M.] Univ Guelph, Dept Mol & Cell Biol, Guelph, ON N1G 2W1, Canada. [Dangl, G. S.] Univ Calif Davis, Fdn Plant Serv, Davis, CA 95616 USA. RP Aradhya, M (reprint author), USDA ARS Natl Clonal Germplasm Repository, 1 Shields Ave UC Davis, Davis, CA 95616 USA. EM aradhya@ucdavis.edu FU U.S. Department of Agriculture, Agricultural Research Service [5306-21000-018-00D] FX This research is supported by the grant 5306-21000-018-00D from the U.S. Department of Agriculture, Agricultural Research Service. We are grateful to Dr. Peter Cousins for providing the plant samples of some of the grape genotypes from his rootstock breeding program. NR 69 TC 9 Z9 9 U1 0 U2 58 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD FEB PY 2013 VL 299 IS 2 BP 317 EP 330 DI 10.1007/s00606-012-0723-4 PG 14 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 074GT UT WOS:000313801600003 ER PT J AU Railsback, SF Harvey, BC AF Railsback, Steven F. Harvey, Bret C. TI Trait-mediated trophic interactions: is foraging theory keeping up? SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Review ID PREDATOR-PREY INTERACTIONS; INDIVIDUAL-BASED MODEL; INTERSPECIFIC INTERACTIONS; COMMUNITY ECOLOGY; FOOD WEBS; TRADE-OFF; BEHAVIOR; POPULATION; DENSITY; RULES AB Many ecologists believe that there is a lack of foraging theory that works in community contexts, for populations of unique individuals each making trade-offs between food and risk that are subject to feedbacks from behavior of others. Such theory is necessary to reproduce the trait-mediated trophic interactions now recognized as widespread and strong. Game theory can address feedbacks but does not provide foraging theory for unique individuals in variable environments. 'State-and prediction-based theory' (SPT) is a new approach that combines existing trade-off methods with routine updating: individuals regularly predict future food availability and risk from current conditions to optimize a fitness measure. SPT can reproduce a variety of realistic foraging behaviors and trait-mediated trophic interactions with feedbacks, even when the environment is unpredictable. C1 [Railsback, Steven F.] Humboldt State Univ, Dept Math, Arcata, CA 95521 USA. [Railsback, Steven F.] Lang Railsback & Associates, Arcata, CA 95521 USA. [Harvey, Bret C.] US Forest Serv, USDA, Pacific SW Res Stn, Arcata, CA 95521 USA. RP Railsback, SF (reprint author), Humboldt State Univ, Dept Math, 1 Harpst St, Arcata, CA 95521 USA. EM steven.railsback@humboldt.edu OI Railsback, Steven/0000-0002-5923-9847 NR 53 TC 24 Z9 24 U1 5 U2 120 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD FEB PY 2013 VL 28 IS 2 BP 119 EP 125 PG 7 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 093FN UT WOS:000315177200011 PM 22995894 ER PT J AU Shephard, GS Berthiller, F Burdaspal, PA Crews, C Jonker, MA Krska, R Lattanzio, VMT MacDonald, S Malone, RJ Maragos, C Sabino, M Solfrizzo, M van Egmond, HP Whitaker, TB AF Shephard, G. S. Berthiller, F. Burdaspal, P. A. Crews, C. Jonker, M. A. Krska, R. Lattanzio, V. M. T. MacDonald, S. Malone, R. J. Maragos, C. Sabino, M. Solfrizzo, M. van Egmond, H. P. Whitaker, T. B. TI Developments in mycotoxin analysis: an update for 2011-2012 SO WORLD MYCOTOXIN JOURNAL LA English DT Article DE aflatoxin; Alternaria; ergot; fumonisin; ochratoxin; patulin; trichothecene; zearalenone; sampling; multimycotoxin; botanicals ID PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; IMMUNOAFFINITY COLUMN CLEANUP; SURFACE-PLASMON RESONANCE; AFLATOXIN M-1 CONTAMINATION; LINKED-IMMUNOSORBENT-ASSAY; THIN-LAYER-CHROMATOGRAPHY; LATERAL FLOW IMMUNOASSAY; DIODE-ARRAY DETECTION AB This review highlights developments in mycotoxin analysis and sampling over a period between mid-2011 and mid-2012. It covers the major mycotoxins aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxin, patulin, trichothecenes, and zearalenone. A section on mycotoxins in botanicals and spices is also included. Methods for mycotoxin determination continue to be developed using a wide range of analytical systems ranging from rapid immunochemical-based methods to the latest advances in mass spectrometry. This review follows the format of previous reviews in this series (i.e. sections on individual mycotoxins), but due to the rapid spread and developments in the field of multimycotoxin methods by liquid chromatography-tandem mass spectrometry, a separate section has been devoted to advances in this area of research. C1 [Shephard, G. S.] MRC, PROMEC Unit, ZA-7505 Tygerberg, South Africa. [Berthiller, F.; Krska, R.] Univ Nat Resources & Life Sci Vienna, Dept Agrobiotechnol IFA Tulln, Ctr Analyt Chem, Christian Doppler Lab Mycotoxin Metab, A-3430 Tulln, Austria. [Berthiller, F.; Krska, R.] Ctr Analyt Chem, A-3430 Tulln, Austria. [Burdaspal, P. A.] Spanish Food Safety & Nutr Agcy, Natl Food Ctr, Majadahonda 28220, Madrid, Spain. [Crews, C.; MacDonald, S.] Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England. [Jonker, M. A.; van Egmond, H. P.] Univ Wageningen & Res Ctr, RIKILT Inst Food Safety, NL-6700 AE Wageningen, Netherlands. [Lattanzio, V. M. T.; Solfrizzo, M.] CNR, Inst Sci Food Prod, I-700126 Bari, Italy. [Malone, R. J.] Tril Analyt Lab, Washington, MO 63090 USA. [Maragos, C.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Sabino, M.] Adolfo Lutz Inst, BR-01246902 Sao Paulo, Brazil. [Whitaker, T. B.] NC State Univ, Dept Biol & Agr Engn, Raleigh, NC 27695 USA. RP Shephard, GS (reprint author), MRC, PROMEC Unit, POB 19070, ZA-7505 Tygerberg, South Africa. EM gordon.shephard@mrc.ac.za OI Shephard, Gordon Seymour/0000-0002-1267-9036 NR 233 TC 34 Z9 34 U1 3 U2 149 PU WAGENINGEN ACADEMIC PUBLISHERS PI WAGENINGEN PA PO BOX 220, WAGENINGEN, 6700 AE, NETHERLANDS SN 1875-0710 J9 WORLD MYCOTOXIN J JI World Mycotoxin J. PD FEB PY 2013 VL 6 IS 1 BP 3 EP 30 DI 10.3920/WMJ2012.1492 PG 28 WC Food Science & Technology; Mycology; Toxicology SC Food Science & Technology; Mycology; Toxicology GA 095XB UT WOS:000315367200002 ER PT J AU Cheniany, M Ebrahimzadeh, H Vahdati, K Preece, JE Masoudinejad, A Mirmasoumi, M AF Cheniany, Monireh Ebrahimzadeh, Hassan Vahdati, Kourosh Preece, John E. Masoudinejad, Ali Mirmasoumi, Masoud TI Content of different groups of phenolic compounds in microshoots of Juglans regia cultivars and studies on antioxidant activity SO ACTA PHYSIOLOGIAE PLANTARUM LA English DT Article DE In vitro; Juglone; Micropropagation; Quercetin; Walnut ID FLAVONOID CONTENT; WALNUT; EXTRACTS; LEAVES; L.; MICROCUTTINGS; SHOOTS; FRUITS; ADULT; TREES AB Phenolic and other compounds were extracted from micropropagated axillary shoots (microshoots) of the walnut (Juglans regia L.) cultivars 'Chandler', 'Howard', 'Kerman', 'Sunland', and 'Z63'. Among cultivars, microshoots showed differences in phenolic compounds, phenolic acids, flavonoids, and proanthocyanidins. All cultivars contained the phenolics acids chlorogenic acid, gallic acid, p-coumaric acid; the naphthoquinone juglone; and the flavonoid quercetin. The phenolic acids syringic acid and vanillin were present only in microshoots of 'Howard'. Microshoot extracts had different antioxidant activity with 'Kerman' the highest and 'Chandler' the lowest in each of three antioxidant assays: the phospho-molybdenum assay (PPM), reducing power assay, and 2,2diphenyl-1-picrylhydrazyl-scavenging effect. There was a strong linear relationship between total phenolic compound content of microshoots and increasing antioxidant activity. C1 [Cheniany, Monireh; Ebrahimzadeh, Hassan; Mirmasoumi, Masoud] Univ Tehran, Sch Biol, Coll Sci, Plant Physiol Lab,Dept Bot, Tehran 141556455, Iran. [Vahdati, Kourosh] Univ Tehran, Dept Hort, Coll Abooraihan, Tehran 141556455, Iran. [Preece, John E.] Univ Calif Davis, USDA ARS, Davis, CA 95616 USA. [Masoudinejad, Ali] Univ Tehran, Inst Biochem & Biophys, Tehran 141556455, Iran. RP Cheniany, M (reprint author), Univ Tehran, Sch Biol, Coll Sci, Plant Physiol Lab,Dept Bot, Tehran 141556455, Iran. EM cheniany@khayam.ut.ac.ir NR 26 TC 6 Z9 6 U1 1 U2 28 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0137-5881 J9 ACTA PHYSIOL PLANT JI Acta Physiol. Plant. PD FEB PY 2013 VL 35 IS 2 BP 443 EP 450 DI 10.1007/s11738-012-1087-7 PG 8 WC Plant Sciences SC Plant Sciences GA 091FN UT WOS:000315034900014 ER PT J AU Yang, ZH Sun, R Grinchuk, V Blanco, JAF Notari, L Bohl, JA McLean, LP Ramalingam, TR Wynn, TA Urban, JF Vogel, SN Shea-Donohue, T Zhao, AP AF Yang, Zhonghan Sun, Rex Grinchuk, Viktoriya Blanco, Joan Antoni Fernandez Notari, Luigi Bohl, Jennifer A. McLean, Leon P. Ramalingam, Thirumalai R. Wynn, Thomas A. Urban, Joseph F., Jr. Vogel, Stefanie N. Shea-Donohue, Terez Zhao, Aiping TI IL-33-induced alterations in murine intestinal function and cytokine responses are MyD88, STAT6, and IL-13 dependent SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE IL-33; intestinal function; IL-13; STAT6; MYD88 ID EPITHELIAL-CELL FUNCTION; NIPPOSTRONGYLUS-BRASILIENSIS INFECTION; ALTERNATIVELY ACTIVATED MACROPHAGES; SMOOTH-MUSCLE-CELLS; AIRWAY INFLAMMATION; HELMINTH INFECTION; TYPE-2 IMMUNITY; ENTERIC NERVES; LYMPHOID-CELLS; MAST-CELLS AB Yang Z, Sun R, Grinchuk V, Fernandez Blanco JA, Notari L, Bohl JA, McLean LP, Ramalingam TR, Wynn TA, Urban JF, Vogel SN, Shea-Donohue T, Zhao A. IL-33-induced alterations in murine intestinal function and cytokine responses are MyD88, STAT6, and IL-13 dependent. Am J Physiol Gastrointest Liver Physiol 304: G381-G389, 2013. First published December 20, 2012; doi: 10.1152/ajpgi.00357.2012.-IL-33 is a recently identified cytokine member of the IL-1 family. The biological activities of IL-33 are associated with promotion of Th2 and inhibition of Th1/Th17 immune responses. Exogenous IL-33 induces a typical "type 2" immune response in the gastrointestinal tract, yet the underlying mechanisms remain to be fully elucidated. In addition, the role of IL-33 in the regulation of gastrointestinal function is not known. The present study investigated IL-33-dependent intestinal immunity and function in mice. Exogenous IL-33 induced a polarized type 2 cytokine response in the intestine that was entirely MyD88 dependent but STAT6 and IL-13 independent. Mice injected with recombinant IL-33 exhibited intestinal smooth muscle hypercontractility, decreased epithelial responses to acetylcholine and glucose, and increased mucosal permeability. IL-33 effects on intestinal epithelial function were STAT6 dependent, and both IL-4 and IL-13 appeared to play a role. The effects on smooth muscle function, however, were attributable to both STAT6-dependent and -independent mechanisms. In addition, IL-13 induction of insulin-like growth factor-1 was implicated in IL-33-induced smooth muscle hypertrophy. Finally, alternative activation of macrophages induced by IL-33 revealed a novel pathway that is IL-4, IL-13, and STAT6 independent. Thus manipulating IL-33 or related signaling pathways represents a potential therapeutic strategy for treating inflammatory diseases associated with dysregulated intestinal function. C1 [Yang, Zhonghan; Sun, Rex; Grinchuk, Viktoriya; Blanco, Joan Antoni Fernandez; Notari, Luigi; Bohl, Jennifer A.; McLean, Leon P.; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Yang, Zhonghan; Sun, Rex; Grinchuk, Viktoriya; Blanco, Joan Antoni Fernandez; Notari, Luigi; Bohl, Jennifer A.; McLean, Leon P.; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Mucosal Biol Res Ctr, Baltimore, MD 21201 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Ramalingam, Thirumalai R.; Wynn, Thomas A.] NIAID, Div Parasitol, NIH, Bethesda, MD 20892 USA. [Vogel, Stefanie N.] Univ Maryland, Sch Med, Dept Microbiol Immunol, Baltimore, MD 21201 USA. RP Zhao, AP (reprint author), Univ Maryland, Sch Med, Dept Med, 20 Penn St,HSF 2,R S349, Baltimore, MD 21201 USA. EM azhao@mbrc.umaryland.edu OI Urban, Joseph/0000-0002-1590-8869 FU National Institutes of Health [DK-083418, AI/DK-49316, AI-18797]; USDA CRIS [1235-51000-055] FX This work was supported by National Institutes of Health Grants DK-083418 (A. Zhao), AI/DK-49316 (T. Shea-Donohue), and AI-18797 (S. N. Vogel) and USDA CRIS project no. 1235-51000-055 (J. F. Urban). The opinions and assertions in this article are those of the authors and do not necessarily represent those of the U.S. Department of Agriculture. NR 47 TC 17 Z9 17 U1 0 U2 10 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD FEB PY 2013 VL 304 IS 4 BP G381 EP G389 DI 10.1152/ajpgi.00357.2012 PG 9 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA 092SW UT WOS:000315143600008 PM 23257921 ER PT J AU Lorch, JM Muller, LK Russell, RE O'Connor, M Lindner, DL Blehert, DS AF Lorch, Jeffrey M. Muller, Laura K. Russell, Robin E. O'Connor, Michael Lindner, Daniel L. Blehert, David S. TI Distribution and Environmental Persistence of the Causative Agent of White-Nose Syndrome, Geomyces destructans, in Bat Hibernacula of the Eastern United States SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FUNGAL PATHOGEN; SPREAD; ORIGIN AB White-nose syndrome (WNS) is an emerging disease of hibernating bats caused by the recently described fungus Geomyces destructans. First isolated in 2008, the origins of this fungus in North America and its ability to persist in the environment remain undefined. To investigate the correlation between manifestation of WNS and distribution of G. destructans in the United States, we analyzed sediment samples collected from 55 bat hibernacula (caves and mines) both within and outside the known range of WNS using a newly developed real-time PCR assay. Geomyces destructans was detected in 17 of 21 sites within the known range of WNS at the time when the samples were collected; the fungus was not found in 28 sites beyond the known range of the disease at the time when environmental samples were collected. These data indicate that the distribution of G. destructans is correlated with disease in hibernating bats and support the hypothesis that the fungus is likely an exotic species in North America. Additionally, we examined whether G. destructans persists in infested bat hibernacula when bats are absent. Sediment samples were collected from 14 WNS-positive hibernacula, and the samples were screened for viable fungus by using a culture technique. Viable G. destructans was cultivated from 7 of the 14 sites sampled during late summer, when bats were no longer in hibernation, suggesting that the fungus can persist in the environment in the absence of bat hosts for long periods of time. C1 [Lorch, Jeffrey M.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Muller, Laura K.; Russell, Robin E.; Blehert, David S.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [O'Connor, Michael] Wisconsin Vet Diagnost Lab, Madison, WI USA. [Lindner, Daniel L.] US Forest Serv, No Res Stn, Ctr Mycol Res, Madison, WI USA. RP Lorch, JM (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. EM jmlorch@wisc.edu; dblehert@usgs.gov OI Russell, Robin/0000-0001-8726-7303; Lorch, Jeffrey/0000-0003-2239-1252 FU National Speleological Society; U.S. Fish and Wildlife Service; U.S. Forest Service; U.S. Geological Survey FX This work was supported by the National Speleological Society, the U.S. Fish and Wildlife Service, the U.S. Forest Service, and the U.S. Geological Survey. NR 30 TC 39 Z9 39 U1 4 U2 158 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2013 VL 79 IS 4 BP 1293 EP 1301 DI 10.1128/AEM.02939-12 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 089EE UT WOS:000314891500026 PM 23241985 ER PT J AU Singh, AK Stanker, LH Sharma, SK AF Singh, Ajay K. Stanker, Larry H. Sharma, Shashi K. TI Botulinum neurotoxin: Where are we with detection technologies? SO CRITICAL REVIEWS IN MICROBIOLOGY LA English DT Review DE Botulism; botulinum neurotoxin; BoNT; detection; mouse bioassay; immunoassay; ELISA; FRET assay; mass spectrometry; PCR; DNA microarray; endopeptidase; flow cytometry; cell based assay ID PHRENIC NERVE-HEMIDIAPHRAGM; SINGLE-DOMAIN ANTIBODIES; LINKED-IMMUNOSORBENT-ASSAY; TOXIN TYPE-A; CLOSTRIDIUM-BOTULINUM; IN-VITRO; MOUSE BIOASSAY; SEROTYPE-A; SMALL NUMBERS; PCR ASSAY AB Because of its high toxicity, botulinum neurotoxin (BoNT) poses a significant risk to humans and it represents a possible biological warfare agent. Nevertheless, BoNT serotypes A and B are considered an effective treatment for a variety of neurological disorders. The growing applicability of BoNT as a drug, and its potential use as a biological threat agent, highlight the urgent need to develop sensitive detection assays and therapeutic counter measures. In the last decade, significant progress has been made in BoNT detection technologies but none have fully replaced the mouse lethality assay, the current "gold standard". Recently, new advances in robotics and the availability of new reagents have allowed development of methods for rapid toxin analysis. These technologies while promising need further refinement. C1 [Singh, Ajay K.; Sharma, Shashi K.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. [Stanker, Larry H.] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. RP Sharma, SK (reprint author), US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. EM shashi.sharma@fda.hhs.gov FU US Department of Homeland Security FX This work was supported by funding from the US Department of Homeland Security to SKS and LHS. The views or opinions presented in this review are those of the authors and should not be construed as an official views of the US Food and Drug Administration or of the USDA. The FDA and USDA are an equal opportunity provider and employer. NR 92 TC 22 Z9 23 U1 3 U2 92 PU INFORMA HEALTHCARE PI NEW YORK PA 52 VANDERBILT AVE, NEW YORK, NY 10017 USA SN 1040-841X J9 CRIT REV MICROBIOL JI Crit. Rev. Microbiol. PD FEB PY 2013 VL 39 IS 1 BP 43 EP 56 DI 10.3109/1040841X.2012.691457 PG 14 WC Microbiology SC Microbiology GA 075CT UT WOS:000313862100004 PM 22676403 ER PT J AU Kirk, WW Hanson, LE Sprague, CL AF Kirk, William W. Hanson, Linda E. Sprague, Christy L. TI Glyphosate and fungicide effects on Cercospora leaf spot in four glyphosate-resistant sugar beet (Beta vulgaris) varieties SO CROP PROTECTION LA English DT Article DE Standard-split; RAUDPC; Healthy sugar beet; Harvestable; Strobilurins; Triazole ID SUDDEN-DEATH-SYNDROME; DEFENSE-MECHANISMS; ROOT-ROT; HERBICIDE; DISEASES; SOLANI; RHIZOCTONIA; BETICOLA; PATHWAY; COTTON AB The potential for improved management of Cercospora leaf spot (CLS), caused by Cercospora bed cola, using the herbicide glyphosate in glyphosate-resistant sugar beet varieties was investigated. Controlled field experiments were conducted in 2008 and 2009 to determine if glyphosate and glyphosate fungicide combinations improved the management of CLS in four commercial varieties of glyphosate-resistant sugar beet. Variety and fungicide main effects were significant for CLS development. However, regardless of the herbicide program, glyphosate or a conventional herbicide program, CLS development was not affected. Therefore, results from of this research indicate that glyphosate and glyphosate fungicide combinations do not significantly contribute to CLS management. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Kirk, William W.; Hanson, Linda E.] Michigan State Univ, Dept Plant Pathol, E Lansing, MI 48824 USA. [Hanson, Linda E.] Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. [Sprague, Christy L.] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA. RP Kirk, WW (reprint author), Michigan State Univ, Dept Plant Pathol, 35 Plant Biol Bldg, E Lansing, MI 48824 USA. EM kirkw@msu.edu FU Michigan AgBioResearch GREEEN (Generating Research and Extension to meet Economic and Environmental Needs); Michigan Sugar Company FX The authors would like to thank Gary Powell, Tom Goodwill, Tim Duckert, and Paul Horny for assistance with this project. This research was funded, in part, by the Michigan AgBioResearch GREEEN (Generating Research and Extension to meet Economic and Environmental Needs) and the Michigan Sugar Company. NR 39 TC 0 Z9 0 U1 3 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD FEB PY 2013 VL 44 BP 38 EP 43 DI 10.1016/j.cropro.2012.09.016 PG 6 WC Agronomy SC Agriculture GA 071RG UT WOS:000313611300006 ER PT J AU Blersch, DM Kangas, PC Mulbry, W AF Blersch, David M. Kangas, Patrick C. Mulbry, Walter TI Autonomous Benthic Algal Cultivator Under Feedback Control of Ecosystem Metabolism SO ENVIRONMENTAL ENGINEERING SCIENCE LA English DT Article DE automation; benthic algal cultivation; ecosystem metabolism; feedback control; net primary production; technoecosystem; turbulence ID PERIPHYTON; GROWTH; PRODUCTIVITY; SUBSIDY AB An autonomous technoecological hybrid was developed that controls primary production of algae in a laboratory-scale cultivator. The technoecosystem was based on a benthic algal cultivator that combined engineered feedback control programming with ecosystem-level feedback parameters. The feedback control system was designed to measure primary production of benthic algal turf through continuous pH monitoring and then automatically manipulate flow turbulence to optimize for maximum productivity. Results showed that the system converged on optimum productivity at the lowest level of turbulence, a state of optimum efficiency for power acquisition as predicted for self-organizing systems. Observations on species composition of the dominant algae showed shifting relative abundance of species for automated feedback trials, suggesting that certain species contribute to system organization through interfacing with the feedback control system. This work presents an original design for an experimental technoecosystem, and has the impact of advancing an experimental concept for testing principles of organization for self-organizing systems. C1 [Blersch, David M.] SUNY Buffalo, Dept Civil Struct & Environm Engn, Buffalo, NY 14260 USA. [Kangas, Patrick C.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Mulbry, Walter] ARS, USDA, Beltsville, MD USA. RP Blersch, DM (reprint author), SUNY Buffalo, Dept Civil Struct & Environm Engn, 212 Ketter Hall, Buffalo, NY 14260 USA. EM dblersch@buffalo.edu RI Blersch, David/L-7490-2015 FU USDA Food and Agricultural Sciences National Needs Graduate Fellowship Grants Program FX We gratefully acknowledge Shannon Kondrad Ingram of the USDA ARS EMBUL for help with experiments. Dr. David Tilley of the Environmental Science and Technology Department at the University of Maryland provided technical advice and review for this research. Support for D.M.B. during these experiments was provided by the USDA Food and Agricultural Sciences National Needs Graduate Fellowship Grants Program. NR 24 TC 0 Z9 0 U1 1 U2 8 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1092-8758 J9 ENVIRON ENG SCI JI Environ. Eng. Sci. PD FEB PY 2013 VL 30 IS 2 BP 53 EP 60 DI 10.1089/ees.2012.0045 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 090KW UT WOS:000314979000002 ER PT J AU Shrestha, SL Casey, FXM Hakk, H Padmanabhan, G AF Shrestha, Suman L. Casey, Francis X. M. Hakk, Heldur Padmanabhan, G. TI Radioassay-Based Approach to Investigate Fate and Transformation of Conjugated and Free Estrogens in an Agricultural Soil SO ENVIRONMENTAL ENGINEERING SCIENCE LA English DT Article DE agricultural soils; environmental fate; estrogens; estrogen conjugates; radioisotopes; 17 beta-estradiol-3-glucuronide ID DRINKING-WATER; HORMONES; URINE; 17-BETA-ESTRADIOL; PHARMACEUTICALS; CHROMATOGRAPHY; CONTAMINANTS; PERSISTENCE; ENVIRONMENT; TRANSPORT AB Estrogens, a potent group of endocrine disruptors toward aquatic species, are primarily excreted as conjugates from humans and animals. Radioassay-based approaches with detailed speciation have been frequently conducted for environmental-fate studies for pesticides; however, such techniques have not been exploited for reproductive hormones, and especially for hormone conjugates. This article describes a simple, robust, and high-mass-recovery approach to investigate the fate and transformation of a prototype estrogen conjugate, that is, 17 beta-estradiol-3-glucuronide (E2-3G), and its metabolites (free estrogens) in a laboratory soil and water matrix without the need for enzymatic cleavage and/or fluorescent derivatization. E2-3G and its metabolites were baseline resolved in a single run using high-performance liquid chromatography (HPLC) and quantified by liquid scintillation counting of the HPLC effluents. Transformation of E2-3G and the disposition of its metabolites-the free estrogens 17 beta-estradiol and estrone-into aqueous, sorbed, and gaseous phases, were adequately accounted for in a soil-water batch system. High mass balances ranging from 99.0% to 114.1% were obtained. Although the method gave lower sensitivity (parts per billion) than tandem mass spectrometer (parts per trillion), it offered sufficient chromatographic resolution and sensitivity to study the fate of labile estrogens in environmental matrices, using the concentration range of this study. An additional advantage of the approach was the relatively low cost of the instrumentation employed. The presented approach can be successfully applied to study the fate of conjugated hormones and their metabolites in the environment allowing simultaneous discernment of complex fate and transformation processes in soil, water, and gas. C1 [Shrestha, Suman L.; Casey, Francis X. M.] N Dakota State Univ, Dept Soil Sci, Fargo, ND 58018 USA. [Padmanabhan, G.] N Dakota State Univ, Dept Civil Engn, Fargo, ND 58018 USA. [Hakk, Heldur] ARS, Biosci Res Lab, USDA, Fargo, ND USA. RP Casey, FXM (reprint author), N Dakota State Univ, Dept Soil Sci, Fargo, ND 58018 USA. EM francis.casey@ndsu.edu RI Casey, Francis/A-2135-2010 OI Casey, Francis/0000-0002-6035-7234 FU USDA-NIFA through an AFRI Competative Grant [2010-65102-204000] FX We thank Dr. David Smith (USDA-ARS) for making available his laboratory for this research and also providing his valuable suggestions. We also thank Colleen Pfaff, Dee Anne Ellig, Barb Magelky, Mike Giddings, Grant Harrington, Jason Holthusen (USDA-ARS), and Nathan Derby (Soil Science Department, North Dakota State University) for their support in the laboratory work and Glenn Wittenberg (USDA-ARS) for his IT support. This research was based upon work supported by the USDA-NIFA through an AFRI Competative Grant (No. 2010-65102-204000). NR 29 TC 2 Z9 2 U1 2 U2 34 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1092-8758 J9 ENVIRON ENG SCI JI Environ. Eng. Sci. PD FEB PY 2013 VL 30 IS 2 BP 89 EP 96 DI 10.1089/ees.2012.0206 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 090KW UT WOS:000314979000007 PM 23443733 ER PT J AU Luo, J Yu, Y Mitra, A Chang, S Zhang, HM Liu, G Yang, N Song, JZ AF Luo, Juan Yu, Ying Mitra, Apratim Chang, Shuang Zhang, Huanmin Liu, George Yang, Ning Song, Jiuzhou TI Genome-Wide Copy Number Variant Analysis in Inbred Chickens Lines With Different Susceptibility to Marek's Disease SO G3-GENES GENOMES GENETICS LA English DT Article DE CNV; disease resistance; Marek's disease; chicken ID LOCI AFFECTING SUSCEPTIBILITY; WHITE LEGHORN CHICKENS; GENE-EXPRESSION; VIRUS-INFECTION; LAYER CHICKENS; RESISTANCE; POPULATION; DIVERSITY; EVOLUTION; EFFICACY AB Breeding of genetically resistant chickens to Marek's disease (MD) is a vital strategy to poultry health. To find the markers underlying the genetic resistance to MD, copy number variation (CNV) was examined in inbred MD-resistant and -susceptible chicken lines. A total of 45 CNVs were found in four lines of chickens, and 28 were potentially involved in immune response and cell proliferation, etc. Importantly, two CNVs related with MD resistance were transmitted to descendent recombinant congenic lines that differ in susceptibility to MD. Our findings may lead to better strategies for genetic improvement of disease resistance in poultry. C1 [Luo, Juan; Yu, Ying; Mitra, Apratim; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Chang, Shuang; Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Liu, George] ARS, Bovine Funct Genom Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Yang, Ning] China Agr Univ, Coll Anim Sci, Dept Anim Breeding & Genet, Beijing 100193, Peoples R China. RP Song, JZ (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM songj88@umd.edu FU U.S. Department of Agriculture (USDA)-Nation Research Initiative/National Institute of Food and Agriculture (NRI/NIFA) [2008-35204-04660]; USDA-NRI/NIFA [2010-65205-20588] FX The work was supported by U.S. Department of Agriculture (USDA)-Nation Research Initiative/National Institute of Food and Agriculture (NRI/NIFA) 2008-35204-04660 and USDA-NRI/NIFA 2010-65205-20588. J.L. analyzed the aCGH data, performed validation experiments, and wrote the paper. Y.Y. extracted DNA. S. C. and H.M.Z. collected samples and revised the paper. N.Y. and H.M.Z. revised the paper. J.Z.S. designed the experiments and revised the paper. NR 40 TC 11 Z9 11 U1 3 U2 10 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD FEB 1 PY 2013 VL 3 IS 2 BP 217 EP 223 DI 10.1534/g3.112.005132 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA 089AY UT WOS:000314881600007 PM 23390598 ER PT J AU Timofejeva, L Skibbe, DS Lee, S Golubovskaya, I Wang, R Harper, L Walbot, V Cande, WZ AF Timofejeva, Ljudmilla Skibbe, David S. Lee, Sidae Golubovskaya, Inna Wang, Rachel Harper, Lisa Walbot, Virginia Cande, William Zacheus TI Cytological Characterization and Allelism Testing of Anther Developmental Mutants Identified in a Screen of Maize Male Sterile Lines SO G3-GENES GENOMES GENETICS LA English DT Article DE maize; anther development; cell fate acquisition; male sterility ID PROGRAMMED CELL-DEATH; TRANSCRIPTION FACTOR; GENE ENCODES; ZEA-MAYS; ARABIDOPSIS ANTHER; POLLEN DEVELOPMENT; TAPETUM-DEGENERATION; LEAF DEVELOPMENT; RECEPTOR KINASE; MUTATION ALTERS AB Proper regulation of anther differentiation is crucial for producing functional pollen, and defects in or absence of any anther cell type result in male sterility. To deepen understanding of processes required to establish premeiotic cell fate and differentiation of somatic support cell layers a cytological screen of maize male-sterile mutants has been conducted which yielded 42 new mutants including 22 mutants with premeiotic cytological defects (increasing this class fivefold), 7 mutants with postmeiotic defects, and 13 mutants with irregular meiosis. Allelism tests with known and new mutants confirmed new alleles of four premeiotic developmental mutants, including two novel alleles of msca1 and single new alleles of ms32, ms8, and ocl4, and two alleles of the postmeiotic ms45. An allelic pair of newly described mutants was found. Premeiotic mutants are now classified into four categories: anther identity defects, abnormal anther structure, locular wall defects and premature degradation of cell layers, and/or microsporocyte collapse. The range of mutant phenotypic classes is discussed in comparison with developmental genetic investigation of anther development in rice and Arabidopsis to highlight similarities and differences between grasses and eudicots and within the grasses. C1 [Timofejeva, Ljudmilla; Lee, Sidae; Golubovskaya, Inna; Wang, Rachel; Harper, Lisa; Cande, William Zacheus] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA. [Timofejeva, Ljudmilla; Skibbe, David S.] Tallinn Univ Technol, Dept Gene Technol, EE-12618 Tallinn, Estonia. [Walbot, Virginia] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. [Golubovskaya, Inna] NI Vavilov Inst Plant Ind, St Petersburg 190000, Russia. [Harper, Lisa] ARS, Ctr Plant Gene Express, USDA, Albany, CA 94710 USA. RP Cande, WZ (reprint author), Univ Calif Berkeley, Dept Mol & Cell Biol, 341 Life Sci Addit, Berkeley, CA 94720 USA. EM zcande@berkeley.edu FU U.S. National Science Foundation [PGRP 07-01880] FX We thank Jay Hollick (Ohio State University) and Maize Genetics Cooperation Stock Center for providing us with male sterile lines. We also thank Jihyun Moon (UC Berkeley) for comments on the manuscript. This work was supported by a grant from the U.S. National Science Foundation (PGRP 07-01880). NR 89 TC 14 Z9 17 U1 2 U2 16 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD FEB 1 PY 2013 VL 3 IS 2 BP 231 EP 249 DI 10.1534/g3.112.004465 PG 19 WC Genetics & Heredity SC Genetics & Heredity GA 089AY UT WOS:000314881600009 PM 23390600 ER PT J AU Jones, G Teal, P Henrich, VC Krzywonos, A Sapa, A Wozniak, M Smolka, J Jones, D AF Jones, Grace Teal, Peter Henrich, Vincent C. Krzywonos, Anna Sapa, Agnes Wozniak, Mietek Smolka, John Jones, Davy TI Ligand binding pocket function of Drosophila USP is necessary for metamorphosis SO GENERAL AND COMPARATIVE ENDOCRINOLOGY LA English DT Article DE Methyl farnesoate; Ultraspiracle; Retinoid X receptor; Juvenile hormone ID RETINOID-X-RECEPTOR; JUVENILE-HORMONE ACTION; CELL-CULTURE-SYSTEM; ECDYSONE RECEPTOR; NUCLEAR RECEPTOR; METHOPRENE-TOLERANT; 9-CIS-RETINOIC ACID; LARVAL DEVELOPMENT; METHYL FARNESOATE; MASS-SPECTROMETRY AB The widely accepted paradigm that epoxidized methyl farnesoates ("juvenile hormones," JHs) are the principal sesquiterpenoid hormones regulating insect metamorphosis was assessed in Drosophila melanogaster. GC-MS analysis of circulating methyl farnesoids during the mid to late 3rd instar showed that methyl farnesoate is predominant over methyl epoxyfarnesoate (=JH III). The circulating concentration of methyl farnesoate (reaching nearly 500 nM), was easily high enough on a kinetic basis to load the Drosophila ortholog of the nuclear hormone receptor RXR (also known as "ultraspiracle," USP), whereas the circulating concentrations of JH III and methyl bisepoxyfarnesoate (bisepoxyJH III) were not. The hypothesis that the ligand pocket of USP necessarily binds an endogenous ligand for differentiation of the immature to the adult was tested with USP mutated at residue that normally extends a side chain into the ligand binding pocket. An equilibrium binding assay confirmed that the mutation (Q288A) strongly altered methyl farnesoate interaction with USP, while a heterologous cell-line transfection assay confirmed that the mutation did not allosterically alter the transcriptional response of the ultraspiracle/ecdysone receptor heterodimer to ecdysteroid signaling. Transgenic wildtype USP driven by the cognate natural promoter rescued null animals to develop to the adult inside a normally formed puparium, while in contrast animals transgenically expressing instead the ligand pocket mutant exhibited developmental derangement at the larval to pupal transition, including failure to form a properly shaped or sclerotized puparium. Other point mutations to the pocket strongly reducing affinity for methyl farnesoate similarly disrupted the larval to pupal metamorphosis. These results suggest that normal larval to pupal maturation in this mecopteran model insect requires the involvement of a distinct endocrine axis of USP binding to its own endogenous terpenoid ligand. (c) 2012 Elsevier Inc. All rights reserved. C1 [Jones, Grace; Smolka, John] Univ Kentucky, Dept Biol, Lexington, KY 40504 USA. [Teal, Peter] ARS, USDA, Chem Res Unit, Gainesville, FL 32608 USA. [Henrich, Vincent C.] Univ N Carolina, Ctr Biotechnol Genom & Hlth Res, Greensboro, NC 27402 USA. [Krzywonos, Anna; Sapa, Agnes; Wozniak, Mietek] Wroclaw Med Univ, Dept Clin Chem, Wroclaw, Poland. [Jones, Davy] Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40504 USA. RP Jones, G (reprint author), Univ Kentucky, Dept Biol, Lexington, KY 40504 USA. EM gjones@uky.edu; djones@uky.edu FU National Science Foundation [1052142]; National Institutes of Health [GM075248-04] FX This research was supported in part by the National Science Foundation (1052142) and the National Institutes of Health (GM075248-04). The mutant usp2 line was kindly provided by Nobert Perrimon. We also thank the two anonymous reviewers, and Associate Editor, for their helpful suggestions that improved the manuscript. NR 73 TC 16 Z9 16 U1 1 U2 33 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0016-6480 J9 GEN COMP ENDOCR JI Gen. Comp. Endocrinol. PD FEB 1 PY 2013 VL 182 BP 73 EP 82 DI 10.1016/j.ygcen.2012.11.009 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 090XK UT WOS:000315013100009 PM 23211750 ER PT J AU Levi, A Thies, JA Wechter, WP Harrison, HF Simmons, AM Reddy, UK Nimmakayala, P Fei, ZJ AF Levi, Amnon Thies, Judy A. Wechter, W. Patrick Harrison, Howard F. Simmons, Alvin M. Reddy, Umesh K. Nimmakayala, Padma Fei, Zhangjun TI High frequency oligonucleotides: targeting active gene (HFO-TAG) markers revealed wide genetic diversity among Citrullus spp. accessions useful for enhancing disease or pest resistance in watermelon cultivars SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Citrullus; DNA; Genetic structure; Resistance; Grafting; Nematodes; Fusarium ID ROOT-KNOT NEMATODE; HETEROTIC GROUPS; LANATUS LANDRACES; POWDERY MILDEW; RACE 2; GERMPLASM; FRUIT; IDENTIFICATION; ANTHRACNOSE; PERFORMANCE AB There is a continuous need to enhance watermelon cultivars for disease and pest resistance. Different U.S. Plant Introductions (PIs) of Citrullus lanatus subsp. lanatus var. lanatus [also known as C. lanatus (Thunb.) Matsum. et Nakai subsp. lanatus var. citroides (Bailey) Mansf. ex Greb.] (CLC) collected in southern Africa are a useful source for enhancing disease or pest resistance in watermelon cultivars. They are also valuable as rootstocks for grafted watermelon, particularly in fields infested with root-knot nematodes or Fusarium wilt. However, there is little information about genetic relationships among these PIs. In this study, genetic diversity was examined among 74 CLC PIs collected from their center of origin in southern Africa. Also, 15 Citrullus lanatus subsp. lanatus (CLL) PIs and the American heirloom cultivars Charleston Gray and Black Diamond (Citrullus lanatus subsp. vulgaris (Schrader ex Eckl. et Zeyh.) Fursa) (CLV) and five Citrullus colocynthis (L.) Schrader (CC) PIs collected in different locations throughout the world were used as out-groups in the phylogenetic analysis for the CLC PIs. Twenty-three high frequency oligonucleotides-targeting active gene (HFO-TAG) primers were used in polymerase chain reaction (PCR) experiments to produce a total of 562 polymorphic markers among the Citrullus PIs and cultivars. Cluster and multidimensional scaling plot analysis produced distinct groups of CLC, CLL, and CC PIs. Several PIs that were designated as CLC or CLL were in transitional positions, indicating that they are the result of gene flow between the major Citrullus groups or subgroups. Population structure analysis indicated that CLC comprises two subgroups; each containing a set of unique alleles. Also, unique alleles exist in the CLL and the CC genotypes. Overall, broad genetic diversity exists among the Citrullus PIs. The data in this study should be useful for identifying PIs with a wide genetic distance between them that could be used in breeding programs aiming to develop heterotic F-1 hybrid rootstock lines for grafted watermelon. C1 [Levi, Amnon; Thies, Judy A.; Wechter, W. Patrick; Harrison, Howard F.; Simmons, Alvin M.] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. [Reddy, Umesh K.; Nimmakayala, Padma] W Virginia State Univ, Dept Biol, Douglass Land Grant Inst, Institute, WV 25112 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Levi, A (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Amnon.Levi@ars.usda.gov NR 54 TC 21 Z9 21 U1 0 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2013 VL 60 IS 2 BP 427 EP 440 DI 10.1007/s10722-012-9845-3 PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 081ZO UT WOS:000314361600003 ER PT J AU Ji, K Zhang, DP Motilal, LA Boccara, M Lachenaud, P Meinhardt, LW AF Ji, Kun Zhang, Dapeng Motilal, Lambert A. Boccara, Michel Lachenaud, Philippe Meinhardt, Lyndel W. TI Genetic diversity and parentage in farmer varieties of cacao (Theobroma cacao L.) from Honduras and Nicaragua as revealed by single nucleotide polymorphism (SNP) markers SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Cacao landraces; Chocolate; Conservation; Germplasm; Genetic diversity; Molecular markers; Mesoamerica; Theobroma cacao; Tropical tree ID INTERNATIONAL-COCOA-GENEBANK; NATURAL-POPULATIONS; TRINIDAD AB Cacao (Theobroma cacao L.) is the main source for chocolate with an annual production of four million tons worldwide. This Neotropical tree crop was domesticated in Mesoamerica as far back as 3,000 years ago. Knowledge of genetic diversity and population structure in farmer varieties of cacao in the center of domestication is essential for sustainable production of fine-flavored cacao beans and contributes to in situ/on-farm conservation of farmer varieties. Based on 70 single nucleotide polymorphism markers, we analyzed 84 fine-flavored farmer varieties collected from traditional cacao farms in Honduras and Nicaragua. The study also included 31 clones from the international cacao collections to serve as references. The SNP based multilocus matching identified six synonymous groups, including 14 Criollo and two Amelonado varieties. A moderately high level of genetic diversity was observed in these farmer varieties, indicating the possibility to further explore intra-population variation and breed for fine-flavored cocoa. Multivariate analysis showed clustering of the 84 farmer accessions in five genetic groups: ancient Criollo, Amelonado, Trinitario (including Nicaragua Trinitario and Honduras Trinitario) and Upper Amazon Forastero (only one accession). The Honduras Trinitario differed from the Nicaragua Trinitario group. The clustering results largely supported the perceived classification of cacao by local farmers and researchers, which was mainly based on morphological traits. However, the well known traditional variety "Indio" in this region was identified as synonymous with Amelonado. Parentage analysis showed that the variety "Indio" (or Amelonado) contributed more to the Trinitario type farmer varieties, whereas ancient Criollo had less influence. The present study demonstrates the efficacy of using a small set of SNP makers for cacao germplasm characterization, and further depicts the diverse origins and parentage in farmer varieties from Mesoamerica. This information thus will be highly useful for conservation and utilization of cacao germplasm from this region. C1 [Ji, Kun; Zhang, Dapeng; Meinhardt, Lyndel W.] USDA ARS, Beltsville Agr Res Ctr, PSI, SPCL,BARC W, Beltsville, MD 20705 USA. [Ji, Kun] Southwest Univ, Coll Hort & Landscape, Chongqing 400715, Peoples R China. [Motilal, Lambert A.; Boccara, Michel] Univ W Indies, Cocoa Res Unit, St Augustine, Trinidad, Trinid & Tobago. [Boccara, Michel; Lachenaud, Philippe] Ctr Cooperat Int Rech Agron Dev, Montpellier 5, France. RP Zhang, DP (reprint author), USDA ARS, Beltsville Agr Res Ctr, PSI, SPCL,BARC W, 10300 Baltimore Ave,Bldg 001,Rm 223, Beltsville, MD 20705 USA. EM Dapeng.Zhang@ars.usda.gov NR 42 TC 10 Z9 11 U1 2 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2013 VL 60 IS 2 BP 441 EP 453 DI 10.1007/s10722-012-9847-1 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 081ZO UT WOS:000314361600004 ER PT J AU Bassil, N Boccacci, P Botta, R Postman, J Mehlenbacher, S AF Bassil, Nahla Boccacci, Paolo Botta, Roberto Postman, Joseph Mehlenbacher, Shawn TI Nuclear and chloroplast microsatellite markers to assess genetic diversity and evolution in hazelnut species, hybrids and cultivars SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Corylus; Filbert; Simple sequence repeat (SSR) markers; Universal chloroplast SSRs ID CORYLUS-AVELLANA L.; SUBFAMILY CORYLOIDEAE BETULACEAE; EUROPEAN HAZELNUT; SSR MARKERS; REPEAT MICROSATELLITES; TRINUCLEOTIDE REPEATS; POPULATION-STRUCTURE; HUMAN GENOME; LINKAGE MAP; DNA AB The US Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository in Corvallis, Oregon, preserves more than 800 accessions of hazelnut (Corylus), including C. avellana cultivars and representatives of 10 other recognized shrub and tree species. Characterization and study of genetic diversity in this collection require cross-transferable markers, such as trinucleotide microsatellite or simple sequence repeat (SSR) markers and universal chloroplast SSR markers. We developed new SSR markers and evaluated 114 Corylus accessions representing 11 species and 44 interspecific hybrids. Eight of 23 SSRs generated easy-to-score alleles in all species and seven were highly polymorphic. For those seven, the average heterozygosity was moderate at 0.49, and mean allele number, genetic diversity and polymorphism information index were high at 11.71, 0.79 and 0.76, respectively. The three most polymorphic SSRs were CaC-C008, CaC-C040 and CaC-C118. Neighbor-joining (NJ) clustering and structure analysis agreed with classical taxonomic analysis and supported inclusion of C. maxima within the large polymorphic species, C. avellana. Analysis also indicated that C. californica is a distinct species rather than a botanical variety of C. cornuta. Six universal cpSSRs were polymorphic in Corylus and generated 21 distinct chlorotypes with an average of 3 alleles per locus. Diversity at these cpSSRs was high and ranged from 0.33 to 0.64, with an average of 0.54. Incongruence in NJ topologies between the nuclear and chloroplast markers could be attributed to chloroplast capture related to hybridization during the ancestral diversification of the genus, or to homoplasy. The phylogeographical relationships among the 21 chlorotypes in the 11 Corylus species support Asia as a refugium where several hazelnut lineages survived during glaciation and from which they continued to evolve after dispersal from Asia through the Mediterranean to Europe, and across the Atlantic and/or the Bering land bridge to North America. C1 [Bassil, Nahla; Postman, Joseph] ARS, USDA, Corvallis, OR 97333 USA. [Boccacci, Paolo] UOS Grugliasco, Natl Res Council IVV CNR, Plant Virol Inst, I-10095 Grugliasco, TO, Italy. [Botta, Roberto] Univ Torino, Dipartimento Colture Arboree, I-10095 Grugliasco, TO, Italy. [Mehlenbacher, Shawn] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Bassil, N (reprint author), ARS, USDA, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM nahla.bassil@ars.usda.gov OI Boccacci, Paolo/0000-0001-8574-0478 FU USDA-ARS CRIS [5358-21000-033-00D]; USDA-ARS National Plant Germplasm System Evaluation Grant; Fondazione Cassa di Risparmio di Torino (Italy) FX We acknowledge Barbara Gilmore, Christine Neou-Anderson, and April Nyberg for technical assistance in microsatellite marker separation. Funding for this study was provided by the USDA-ARS CRIS 5358-21000-033-00D, a USDA-ARS National Plant Germplasm System Evaluation Grant, and by the Fondazione Cassa di Risparmio di Torino (Italy). NR 70 TC 11 Z9 13 U1 5 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2013 VL 60 IS 2 BP 543 EP 568 DI 10.1007/s10722-012-9857-z PG 26 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 081ZO UT WOS:000314361600011 ER PT J AU Boza, EJ Irish, BM Meerow, AW Tondo, CL Rodriguez, OA Ventura-Lopez, M Gomez, JA Moore, JM Zhang, DP Motamayor, JC Schnell, RJ AF Boza, Edward J. Irish, Brian M. Meerow, Alan W. Tondo, Cecile L. Rodriguez, Orlando A. Ventura-Lopez, Marisol Gomez, Jaime A. Moore, J. Michael Zhang, Dapeng Motamayor, Juan Carlos Schnell, Raymond J. TI Genetic diversity, conservation, and utilization of Theobroma cacao L.: genetic resources in the Dominican Republic SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Theobroma cacao; Cacao improvement; Gene diversity; Genetic groups; Germplasm mislabeling ID INTERNATIONAL COCOA GENEBANK; POPULATION-STRUCTURE; MICROSATELLITE MARKERS; NATURAL-POPULATIONS; GERMPLASM; TRINIDAD; TRINITARIO; PROGRAM; CLONES; DNA AB Cacao (Theobroma cacao L.) is a significant agricultural commodity in the Dominican Republic, which ranks 11th in the world for cacao exports. To estimate genetic diversity, determine genetic identity, and identify any labeling errors, 14 SSR markers were employed to fingerprint 955 trees among cacao germplasm accessions and local farmer selections (LFS). Comparisons of homonymous plants across plots revealed a significant misidentification rate estimated to be 40.9 % for germplasm accessions and 17.4 % for LFS. The 14 SSRs amplified a total of 117 alleles with a mean allelic richness of 8.36 alleles per locus and average polymorphism information content (PIC) value of 0.67 for the germplasm collection. Similar levels of variation were detected among the LFS where a total of 113 alleles were amplified with a mean of 8.07 alleles per locus and PIC of 0.57. The observed heterozygosity (H-obs) was 0.67 for the germplasm collection and 0.60 for LFS. Based on population structure analysis 43.9 % of the germplasm accessions and 72.1 % of the LFS are predominantly of the Amelonado ancestry. Among these Amelonado, 51.7 % for the germplasm collection and 50.6 % for LFS corresponded to Trinitario hybrid lineage. Criollo ancestry was found in 7.6 and 9.5 % of the germplasm accessions and LFS, respectively. The Contamana, Nacional, and Iquitos backgrounds were also observed in both populations, but the Curaray background was only detected in the germplasm accessions. No PurA(0)s or Guiana ancestry was found in either of the populations. Overall, significant genetic diversity, which could be exploited in the Dominican Republic breeding and selection programs, was identified among the germplasm accessions and LFS. C1 [Boza, Edward J.; Meerow, Alan W.; Tondo, Cecile L.; Moore, J. Michael; Motamayor, Juan Carlos; Schnell, Raymond J.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Irish, Brian M.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Rodriguez, Orlando A.; Ventura-Lopez, Marisol] IDIAF, Mata Larga, San Francisco D, Dominican Rep. [Gomez, Jaime A.] Confederac Nacl Cacaocultores Dominicanos Inc CON, Santo Domingo, Dominican Rep. [Zhang, Dapeng] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Motamayor, Juan Carlos] MARS Inc, Hackettstown, NJ USA. [Schnell, Raymond J.] MARS Inc, Elizabethtown, PA USA. RP Schnell, RJ (reprint author), MARS Inc, Elizabethtown, PA USA. EM Ray.Schnell@effem.com FU USAID; MARS, Inc. [58-6631-6-123]; USDA-ARS SHRS cacao program FX The authors would like to thank Land O'Lakes, Inc., International Development, for their collaborative efforts and partial financial support through the USAID-funded Cooperative Development Program (CDP). We also would like to thank MARS, Inc., for funding Trust Agreement #58-6631-6-123: Genetic Improvement of Theobroma cacao, and the USDA-ARS SHRS cacao program for their partial financial support. We would like to extend our appreciation to Drs. Belinda Martineau and Tomas Ayala-Silva for their constructive comments and suggestions to the manuscript. NR 76 TC 7 Z9 7 U1 2 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2013 VL 60 IS 2 BP 605 EP 619 DI 10.1007/s10722-012-9860-4 PG 15 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 081ZO UT WOS:000314361600014 ER PT J AU El Bouhssini, M Ogbonnaya, FC Chen, M Lhaloui, S Rihawi, F Dabbous, A AF El Bouhssini, M. Ogbonnaya, F. C. Chen, M. Lhaloui, S. Rihawi, F. Dabbous, A. TI Sources of resistance in primary synthetic hexaploid wheat (Triticum aestivum L.) to insect pests: Hessian fly, Russian wheat aphid and Sunn pest in the fertile crescent SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Hessian fly; Primary synthetic wheat; Resistance; Russian wheat aphid; Sunn pest; Triticum aestivum ID EURYGASTER-INTEGRICEPS PUTON; BREAD WHEAT; DIPTERA CECIDOMYIIDAE; DIURAPHIS-NOXIA; D-GENOME; GENES; TAUSCHII; IDENTIFICATION; VIRULENCE; BIOTYPE AB Hessian fly, Mayetiola destructor (Say), Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov), and Sunn pest, Eurygaster integriceps (Puton), are among the most important insect pests of wheat in North Africa, West and Central Asia. Host plant resistance is the most economical, environmentally friendly and practical means of controlling insect pests. Through field and greenhouse screening, several sources of resistance to Hessian fly, RWA and Sunn pest have been identified in wheat and its wild relatives. To further broaden the genetic base of resistance to these pests, 914 fixed lines of synthetic hexaploid wheat (SHW) commonly designated as primary synthetic wheat were evaluated for resistance to Hessian fly, RWA and Sunn pest. The initial screenings for RWA and Sunn pest were carried out in the field at Tel Hadya, Aleppo, Syria, and for Hessian fly in the greenhouse at Tel Hadya during the 2009 and 2010 seasons. Promising accessions from the initial screening for Hessian fly, RWA and Sunn pest were evaluated for confirmation in replicated trials in the greenhouse and field. Fifteen SHWs showed high levels of resistance to Hessian fly and four showed moderate resistance. A wheat line derived from the cross (Triticum turgidum/T. dicoccoides) also showed a high level of resistance to Hessian fly. The level of resistance to RWA in SHW was considerably lower; only one SHW and one durum wheat 'Altar 84' exhibited a high level of resistance, while four SHW were moderately resistant. There were 21 SHW genotypes and one durum wheat 'Langdon' found resistant to Sunn pest feeding at the vegetative stage. Crosses between these potentially novel resistance sources and elite bread wheat were initiated. Genetic and genomic studies using these accessions are ongoing to identify and characterize the resistance genes and reveal potentially new resistance genes, which will be useful in breeding programs to develop wheat germplasm with multiple resistances to these pests. C1 [El Bouhssini, M.; Ogbonnaya, F. C.; Rihawi, F.; Dabbous, A.] ICARDA, Aleppo, Syria. [Chen, M.] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Chen, M.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Lhaloui, S.] INRA, Settat, Morocco. RP El Bouhssini, M (reprint author), ICARDA, Aleppo, Syria. EM M.Bohssini@cgiar.org RI Ogbonnaya, Francis/C-5237-2014 OI Ogbonnaya, Francis/0000-0002-8596-7362 NR 30 TC 10 Z9 10 U1 1 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2013 VL 60 IS 2 BP 621 EP 627 DI 10.1007/s10722-012-9861-3 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 081ZO UT WOS:000314361600015 ER PT J AU Olukolu, BA Negeri, A Dhawan, R Venkata, BP Sharma, P Garg, A Gachomo, E Marla, S Chu, K Hasan, A Ji, JB Chintamanani, S Green, J Shyu, CR Wisser, R Holland, J Johal, G Balint-Kurti, P AF Olukolu, Bode A. Negeri, Adisu Dhawan, Rahul Venkata, Bala P. Sharma, Pankaj Garg, Anshu Gachomo, Emma Marla, Sandeep Chu, Kevin Hasan, Anna Ji, Jiabing Chintamanani, Satya Green, Jason Shyu, Chi-Ren Wisser, Randall Holland, James Johal, Guri Balint-Kurti, Peter TI A Connected Set of Genes Associated with Programmed Cell Death Implicated in Controlling the Hypersensitive Response in Maize SO GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; MULTILOCUS GENOTYPE DATA; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; RUST RESISTANCE; NICOTIANA-BENTHAMIANA; DISEASE-RESISTANCE; CANDIDATE GENES; COMPLEX TRAITS AB Rp1-D21 is a maize auto-active resistance gene conferring a spontaneous hypersensitive response (HR) of variable severity depending on genetic background. We report an association mapping strategy based on the Mutant Assisted Gene Identification and Characterization approach to identify naturally occurring allelic variants associated with phenotypic variation in HR. Each member of a collection of 231 diverse inbred lines of maize constituting a high-resolution association mapping panel were crossed to a parental stock heterozygous for Rp1-D21, and the segregating F-1 generation testcrosses were evaluated for phenotypes associated with lesion severity for 2 years at two locations. A genome-wide scan for associations with HR was conducted with 47,445 SNPs using a linear mixed model that controlled for spurious associations due to population structure. Since the ability to identify candidate genes and the resolution of association mapping are highly influenced by linkage disequilibrium (LD), we examined the extent of genome-wide LD. On average, marker pairs separated by >10 kbp had an r(2) value of <0.1. Genomic regions surrounding SNPs significantly associated with HR traits were locally saturated with additional SNP markers to establish local LD structure and precisely identify candidate genes. Six significantly associated SNPs at five loci were detected. At each locus, the associated SNP was located within or immediately adjacent to candidate causative genes predicted to play significant roles in the control of programmed cell death and especially in ubiquitin pathway-related processes. C1 [Olukolu, Bode A.; Negeri, Adisu; Dhawan, Rahul; Balint-Kurti, Peter] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Holland, James] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Venkata, Bala P.; Sharma, Pankaj; Garg, Anshu; Gachomo, Emma; Marla, Sandeep; Chu, Kevin; Hasan, Anna; Ji, Jiabing; Chintamanani, Satya; Johal, Guri] Purdue Univ, W Lafayette, IN 47907 USA. [Green, Jason; Shyu, Chi-Ren] Univ Missouri, Dept Comp Sci, Columbia, MO 65211 USA. [Shyu, Chi-Ren] Univ Missouri, Inst Informat, Columbia, MO 65211 USA. [Wisser, Randall] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Holland, James; Balint-Kurti, Peter] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Balint-Kurti, P (reprint author), N Carolina State Univ, Dept Plant Pathol, 2572 Thomas Hall, Raleigh, NC 27695 USA. EM peter.balint-kurti@ars.usda.gov OI Holland, James/0000-0002-4341-9675; Balint-Kurti, Peter/0000-0002-3916-194X FU U.S. Department of Agriculture-Agricultural Research Service; Purdue University; National Science Foundation [0822495] FX We thank Major Goodman and the Maize Genetics Cooperation Stock Center for donating seed and David Rhyne, Abbey Sutton, Joe Bundy, Ed Durren, and Donna Stephens for help with fieldwork. We also thank Ed Buckler, Jeff Glaubitz, and the Maize Diversity project team for early access to the genotyping-by-sequencing data set. This work was funded by the U.S. Department of Agriculture-Agricultural Research Service, Purdue University, and by National Science Foundation grant 0822495. NR 80 TC 13 Z9 13 U1 1 U2 25 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 J9 GENETICS JI Genetics PD FEB PY 2013 VL 193 IS 2 BP 609 EP + DI 10.1534/genetics.112.147595 PG 29 WC Genetics & Heredity SC Genetics & Heredity GA 088GH UT WOS:000314821300023 PM 23222653 ER PT J AU Friedman, M Henika, PR Levin, CE AF Friedman, Mendel Henika, Philip R. Levin, Carol E. TI Bactericidal Activities of Health-Promoting, Food-Derived Powders Against the Foodborne Pathogens Escherichia coli, Listeria monocytogenes, Salmonella enterica, and Staphylococcus aureus SO JOURNAL OF FOOD SCIENCE LA English DT Article DE bactericidal activity; dietary supplements; olive; oregano; tea ID INACTIVATE ANTIBIOTIC-RESISTANT; ORGANIC LEAFY GREENS; GROUND-BEEF; CAMPYLOBACTER-JEJUNI; THERMAL-DESTRUCTION; BACILLUS-CEREUS; ANTIBACTERIAL ACTIVITIES; ANTIMICROBIAL ACTIVITY; HETEROCYCLIC AMINES; ESSENTIAL OILS AB We evaluated the relative bactericidal activities (BA50) of 10 presumed health-promoting food-based powders (nutraceuticals) and, for comparison, selected known components against the following foodborne pathogens: Escherichia coli O157:H7, Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus. The relative activities were evaluated using quantitative bactericidal activity [(BA50 value, defined as the percentage of the sample in the assay mixture that resulted in a 50% decrease in colony forming units]. The BA50 values were determined by fitting the data to a sigmoidal curve by regression analysis using concentrationantimicrobial response data. Antimicrobial activity is indicated by a low BA50 value; meaning less material is needed to kill 50% of the bacteria. Olive pomace, olive juice powder, and oregano leaves were active against all 4 pathogens, suggesting that they behave as broad-spectrum antimicrobials. All powders exhibited strong antimicrobial activity against S. aureus. The following powders showed exceptionally high activity against S. aureus (as indicated by the low BA50 values shown in parentheses): apple skin extract (0.002%); olive pomace (0.008%); and grape seed extract (0.016%). Listeria bacteria were also highly susceptible to apple skin extract (0.007%). The most active substances provide candidates for the evaluation of antimicrobial effectiveness in human food and animal feed. Practical Application: Plant-derived health-promoting food supplements, high in bioactive compounds, are candidates for use as antimicrobials in food. C1 [Friedman, Mendel; Henika, Philip R.; Levin, Carol E.] ARS, Western Reg Res Ctr, USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. EM mendel.friedman@ars.usda.gov OI Levin, Carol/0000-0001-6522-6156; Friedman, Mendel/0000-0003-2582-7517 NR 30 TC 15 Z9 15 U1 1 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD FEB PY 2013 VL 78 IS 2 BP M270 EP M275 DI 10.1111/1750-3841.12021 PG 6 WC Food Science & Technology SC Food Science & Technology GA 090PE UT WOS:000314990500020 PM 23317422 ER PT J AU Lu, HYJ Breidt, F Perez-Diaz, I AF Lu, Huiying J. Breidt, Frederick, Jr. Perez-Diaz, Ilenys TI Development of an Effective Treatment for A 5-Log Reduction of Escherichia coli in Refrigerated Pickle Products SO JOURNAL OF FOOD SCIENCE LA English DT Article DE E; coli O157:H7; food safety; fumaric acid; refrigerated pickles; Salmonella ID FUMARIC-ACID; APPLE CIDER; ACIDIFIED CUCUMBERS; O157-H7; SURVIVAL; TOLERANCE; TEMPERATURES; POPULATIONS; STORAGE; YOGURT AB Refrigerated cucumber pickle products cannot be heat processed due to the loss of characteristic sensory attributes. Typically brined refrigerated pickles contain less than 100 mM acetic acid with pH values of 3.7 to 4.0. Refrigeration (4 to 10 degrees C) helps to inhibit the growth of spoilage bacteria and maintain flavor, texture, and appearance of the pickles. Previous research has shown that pathogenic Escherichia coli strains are unusually acid resistant and survive better in refrigerated acid solutions than at higher temperatures. We found that E. coli O157:H7 can survive for 1 mo or longer at 4 degrees C in brines typical of commercial refrigerated pickles. Our objective was to develop methods to assure a 5-log reduction of pathogenic E. coli in these types of products, while maintaining the sensory characteristics. A novel brine formulation was developed, based on current commercial refrigerated pickle brines, which contained 25 mM fumaric acid, 5 mM benzoic acid, 70 mM acetic acid, and 342 mM (2%) sodium chloride, with a pH of 3.8. Sensory data indicate that this formulation did not affect flavor or other sensory attributes of the product, compared to traditional formulations. We achieved a 5-log reduction of E. coli O157:H7 at 30 degrees C for 1.52 +/- 0.15 d, at 20 degrees C for 3.12 +/- 0.34 d, or at 10 degrees C for 8.83 +/- 0.56 d. Growth of lactic acid bacteria was also inhibited. These results can be used by manufacturers to assure a 5-log reduction in cell numbers of E. coli O157:H7 and Salmonella without a heat process during the manufacture of refrigerated pickle products. Practical Application: While refrigerated acidified vegetable products are exempt from the acidified foods regulations, we have shown that the vegetative microbial pathogens E. coli O157:H7 can survive for up to 1 mo in these products, given current commercial production practices. To improve the safety of refrigerated pickle products, a brine formulation with reduced acetic acid, but containing fumaric acid, was developed to assure a 5-log reduction in cell numbers of E. coli O157:H7 without a heat process. The formulation can be used to assure the safety of refrigerated pickled vegetables without altering sensory characteristics. C1 [Lu, Huiying J.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27698 USA. [Breidt, Frederick, Jr.; Perez-Diaz, Ilenys] N Carolina State Univ, ARS, USDA, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. RP Breidt, F (reprint author), N Carolina State Univ, ARS, USDA, SAA Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM fred.breidt@ars.usda.gov NR 27 TC 3 Z9 3 U1 1 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD FEB PY 2013 VL 78 IS 2 BP M264 EP M269 DI 10.1111/j.1750-3841.2012.02968.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 090PE UT WOS:000314990500019 PM 23330823 ER PT J AU Ray, S Jin, T Fan, XT Liu, LS Yam, KL AF Ray, Soumi Jin, Tony Fan, Xuetong Liu, Linshu Yam, Kit L. TI Development of Chlorine Dioxide Releasing Film and Its Application in Decontaminating Fresh Produce SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antimicrobial packaging; chlorine dioxide; decontamination; fresh produce; moisture activation; pathogens; poly-lactic acid film; quality ID ESCHERICHIA-COLI O157-H7; INACTIVATION KINETICS; SALMONELLA-ENTERICA; LISTERIA-MONOCYTOGENES; DRINKING-WATER; SHELF-LIFE; GAS; STRAWBERRIES; EFFICACY; LETTUCE AB A feasibility study was conducted to develop chlorine dioxide (ClO2)-releasing packaging films for decontaminating fresh produce. Sodium chlorite and citric acid powder were incorporated into polylactic acid (PLA) polymer. Films made with different amounts of PLA (100 and 300 mg), percentages of reactant (5% to 60%), and ratios of sodium chlorite to citric acid (1:2 or 2:1) were prepared using a solvent casting method. The release of ClO2 from the resultant films was activated by moisture. Increase of reactants in the films produced more ClO2 while higher PLA content in the films resulted in less release of ClO2. The ratio of sodium chlorite to citric acid and activation temperature (22 degrees C compared with 10 degrees C) did not affect the ClO2 release from the films. Antimicrobial efficacy of ClO2 released from the films was evaluated using grape tomato as a model food. The results indicate that the films were activated by moisture from tomatoes in the package and the released ClO2 reduced Salmonella spp. and Escherichia coli O157:H7 inoculated on the tomatoes to undetectable levels (<5 colony forming units (CFU)/tomato), achieving more than 3 log reduction. The film-treated tomatoes did not show significant changes in color and texture as compared to controls during storage at 10 degrees C for 21 d. This study demonstrated the technical feasibility for development of gaseous ClO2-releasing packaging system to enhance microbial safety and extend shelf life of fresh produce. C1 [Ray, Soumi; Yam, Kit L.] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA. [Jin, Tony; Fan, Xuetong; Liu, Linshu] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Yam, KL (reprint author), Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA. EM yam@aesop.rutgers.edu OI Jin, Tony/0000-0003-0504-5817 NR 37 TC 4 Z9 4 U1 1 U2 63 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD FEB PY 2013 VL 78 IS 2 BP M276 EP M284 DI 10.1111/1750-3841.12010 PG 9 WC Food Science & Technology SC Food Science & Technology GA 090PE UT WOS:000314990500021 PM 23294122 ER PT J AU Ye, XW Scott, T Gao, X Maras, JE Bakun, PJ Tucker, KL AF Ye, Xingwang Scott, Tammy Gao, Xiang Maras, Janice E. Bakun, Peter J. Tucker, Katherine L. TI Mediterranean Diet, Healthy Eating Index 2005, and Cognitive Function in Middle-Aged and Older Puerto Rican Adults SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Mediterranean diet; Healthy Eating Index 2005; Diet quality; Cognitive impairment ID ALZHEIMERS-DISEASE; INFLAMMATORY MARKERS; ADHERENCE; DECLINE; POPULATION; DEMENTIA; RISK; MEN AB Adherence to a Mediterranean diet has recently been shown to protect against cognitive decline and dementia. It remains unclear, however, whether such protection extends to different ethnic groups and middle-aged individuals and how it might compare with adherence to the US Department of Agriculture's 2005 Dietary Guidelines for Americans (measured with Healthy Eating Index 2005 [HEI 2005]). This study examined associations between diet quality, as assessed by the Mediterranean diet and HEI 2005, and cognitive performance in a sample of 1,269 Puerto Rican adults aged 45 to 75 years and living in the Greater Boston area of Massachusetts. Dietary intake was assessed with a food frequency questionnaire specifically designed for and validated with this population. Adherence to the Mediterranean diet was assessed with a 0- to 9-point scale, and the HEI 2005 score was calculated with a maximum score of 100. Cognitive performance was measured with a battery of seven tests and the Mini Mental State Examination was used for global cognitive function. Greater adherence to the Mediterranean diet was associated with higher Mini Mental State Examination score (P trend = 0.012) and lower likelihood (odds ratio=0.87 for each additional point; 95% CI 0.80 to 0.94; P<0.001) of cognitive impairment, after adjustment for confounders. Similarly, individuals with higher HE! 2005 score had higher Mini Mental State Examination score (P trend=0.011) and lower odds of cognitive impairment (odds ratio = 0.86 for each 10 points; 95% CI 0.74 to 0.99; P = 0.033). In conclusion, high adherence to either the Mediterranean diet or the diet recommended by the US Department of Agriculture 2005 Dietary Guidelines for Americans can protect cognitive function in middle-aged and older adults. J Acad Nutr Diet. 2013;113:276-281. C1 [Ye, Xingwang] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai, Peoples R China. [Ye, Xingwang] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Scott, Tammy] Tufts Med Ctr, Dept Psychiat, Boston, MA USA. [Gao, Xiang] Harvard Univ, Sch Publ Hlth, Brigham & Womens Hosp, Dept Nutr,Channing Lab,Dept Med, Boston, MA 02115 USA. [Maras, Janice E.; Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Bakun, Peter J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Dietary Assessment & Epidemiol Res Program, Boston, MA 02111 USA. RP Tucker, KL (reprint author), Northeastern Univ, Dept Hlth Sci, 316 Robinson Hall,360 Huntington Ave, Boston, MA 02115 USA. EM kl.tucker@neu.edu OI Tucker, Katherine/0000-0001-7640-662X FU National Institute on Aging [P01AG023394, R01AG02708]; National Heart, Lung, and Blood Institute [P50HL105185] FX This work was supported by the National Institute on Aging (P01AG023394, R01AG02708) and the National Heart, Lung, and Blood Institute (P50HL105185). NR 29 TC 20 Z9 22 U1 2 U2 32 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2013 VL 113 IS 2 BP 276 EP 281 DI 10.1016/j.jand.2012.10.014 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 086HP UT WOS:000314675000014 PM 23351632 ER PT J AU Raatz, SK Rosenberger, TA Johnson, LK Wolters, WW Burr, GS Picklo, MJ AF Raatz, Susan K. Rosenberger, Thad A. Johnson, LuAnn K. Wolters, William W. Burr, Gary S. Picklo, Matthew J., Sr. TI Dose-Dependent Consumption of Farmed Atlantic Salmon (Salmo salar) Increases Plasma Phospholipid n-3 Fatty Acids Differentially SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE n-3 fatty acids; Salmon; Phospholipid fatty acids ID FISH-OIL; DOCOSAHEXAENOIC ACIDS; OMEGA-3-FATTY-ACIDS; DISEASE; RISK; HUMANS; ADULTS; HEALTH AB Enhanced n-3 fatty acid intake benefits cardiovascular disease (VD) risk reduction. Increasing consumption at a population level may be better addressed by diet than through supplementation. However, limited data are available on the effect of the dose response to fish intake on plasma levels of n-3 fatty acids. To compare the effects of different doses of farmed Atlantic salmon on plasma phospholipid fatty acid proportions and CVD risk biomarkers (eg, glucose, insulin, homeostasis model of assessment-insulin resistance, high-sensitivity C-reactive protein, and interleukin-6) in healthy subjects we performed a randomized three-period crossover-designed trial (4-week treatment, 4- to 8-week washout) to compare the effects of twice per week consumption of farmed Atlantic salmon at doses of 90, 180, and 270 g in 19 apparently healthy men and women (mean age 40 to 65 years) and a body mass index between 25 and 34.9. All study visits were conducted at the US Department of Agriculture Agricultural Research Service Grand Forks Human Nutrition Research Center. Eicosapentaenoic acid and total n-3 concentrations were increased (P<0.05) by all treatments in a dose-response manner, with total n-3 of 8.03%+/- 0.26% and 9.21%+/- 0.26% for 180- and 270-g doses, respectively. Linoleic acid did not change in response to treatment, whereas arachidonic acid (P<0.05) and total n-6 fatty acids decreased dose dependently (<0.0001). The addition of farmed Atlantic salmon to the diet twice per week for 4 weeks at portions of 180 g and 270 g modifies phospholipid fatty acid proportions of n-3 and n-6 in a level associated with decreased risk for CVD. J Acad Nutr Diet. 2013;113:282-287. C1 [Raatz, Susan K.; Johnson, LuAnn K.; Picklo, Matthew J., Sr.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, Minneapolis, MN 55455 USA. [Rosenberger, Thad A.] Univ N Dakota, Dept Pharmacol Physiol & Therapeut, Grand Forks, ND 58201 USA. [Picklo, Matthew J., Sr.] Univ N Dakota, Dept Chem, Grand Forks, ND 58202 USA. [Wolters, William W.; Burr, Gary S.] USDA, Natl Cold Water Marine Aquaculture Ctr, Franklin, ME USA. RP Raatz, SK (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM susan.raatz@ars.usda.gov FU US Department of Agriculture [5450-51000-048-00D, 1915-31000-003-00D]; National institutes of Health [2P20RR017699-09]; Cooke Aquaculture, Blacks Harbor, New Brunswick, Canada FX This work was funded by US Department of Agriculture grant nos. 5450-51000-048-00D and 1915-31000-003-00D; National institutes of Health grant no. 2P20RR017699-09; and Cooke Aquaculture, Blacks Harbor, New Brunswick, Canada. NR 21 TC 12 Z9 12 U1 2 U2 27 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2013 VL 113 IS 2 BP 282 EP 287 DI 10.1016/j.jand.2012.09.022 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 086HP UT WOS:000314675000015 PM 23351633 ER PT J AU Hiza, HAB Casavale, KO Guenther, PM Davis, CA AF Hiza, Hazel A. B. Casavale, Kellie O. Guenther, Patricia M. Davis, Carole A. TI Diet Quality of Americans Differs by Age, Sex, Race/Ethnicity, Income, and Education Level SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Diet quality; Americans; Sociodemographic characteristics ID HEALTHY EATING INDEX-2005; US ADULTS; OF-LIFE; CHILDREN; FOOD; ADOLESCENTS; POPULATION; CONSUMPTION; DISPARITIES; BIOMARKERS AB An index that assesses the multidimensional components of the diet across the lifecycle is useful in describing diet quality. The purpose of this study was to use the Healthy Eating Index-2005, a measure of diet quality in terms of conformance to the 2005 Dietary Guidelines for Americans, to describe the diet quality of Americans by varying sociodemographic characteristics in order to provide insight as to where diets need to improve. The Healthy Eating Index-2005 scores were estimated using 1 day of dietary intake data provided by participants in the 2003-2004 National Health and Nutrition Examination Survey. Mean daily intakes of foods and nutrients, expressed per 1,000 kilocalories, were estimated using the population ratio method and compared with standards that reflect the 2005 Dietary Guidelines for Americans. Participants included 3,286 children (2 to 17 years), 3,690 young and middle-aged adults (18 to 64 years), and 1,296 older adults (65+ years). Results are reported as percentages of maximum scores and tested for significant differences (P <= 0.05) by age, sex, race/ethnicity, income, and education levels. Children and older adults had better:quality diets than younger and middle-aged adults; women had better-quality diets than men; Hispanics had better-quality diets than blacks and whites; and diet quality of adults, but not children, generally improved with income level, except for sodium. The diets of Americans, regardless of socioeconomic status, are far from optimal. Problematic dietary patterns were found among all sociodemographic groups. Major improvements in the nutritional health of the American public can be made by improving eating patterns. J Acad Nutr Diet. 2013;113:297-306. C1 [Hiza, Hazel A. B.; Casavale, Kellie O.; Guenther, Patricia M.; Davis, Carole A.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Hiza, HAB (reprint author), USDA, Ctr Nutr Policy & Promot, 3101 Pk Ctr Dr, Alexandria, VA 22302 USA. EM Hazel.Hiza@cnpp.usda.gov FU US Department of Agriculture/Center for Nutrition Policy and Promotion FX Support for this study was provided by US Department of Agriculture/Center for Nutrition Policy and Promotion. NR 38 TC 85 Z9 86 U1 5 U2 64 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2013 VL 113 IS 2 BP 297 EP 306 DI 10.1016/j.jand.2012.08.011 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 086HP UT WOS:000314675000017 PM 23168270 ER PT J AU Epps, TH Hitchcock, DR Jayakaran, AD Loflin, DR Williams, TM Amatya, DM AF Epps, Thomas H. Hitchcock, Daniel R. Jayakaran, Anand D. Loflin, Drake R. Williams, Thomas M. Amatya, Devendra M. TI Characterization of Storm Flow Dynamics of Headwater Streams in the South Carolina Lower Coastal Plain SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE surface water; groundwater interaction; runoff; watershed management; streamflow; coastal watershed hydrology; first-order stream; hydrograph separation; South Carolina ID PINE PLANTATION; HYDROLOGY; WATER; LINKAGES AB Epps, Thomas H., Daniel R. Hitchcock, Anand D. Jayakaran, Drake R. Loflin, Thomas M. Williams, and Devendra M. Amatya, 2012. Characterization of Storm Flow Dynamics of Headwater Streams in the South Carolina Lower Coastal Plain. Journal of the American Water Resources Association (JAWRA) 1-14. DOI: 10.1111/jawr.12000 Abstract: Hydrologic monitoring was conducted in two first-order lower coastal plain watersheds in South Carolina, United States, a region with increasing growth and land use change. Storm events over a three-year period were analyzed for direct runoff coefficients (ROC) and the total storm response (TSR) as percent rainfall. ROC calculations utilized an empirical hydrograph separation method that partitioned total streamflow into sustained base flow and direct runoff components. ROC ratios ranged from 0 to 0.32 on the Upper Debidue Creek (UDC) watershed and 0 to 0.57 on Watershed 80 (WS80); TSR results ranged from 0 to 0.93 at UDC and 0.01 to 0.74 at WS80. Variability in event runoff generation was attributed to seasonal trends in water table elevation fluctuation as regulated by evapotranspiration. Groundwater elevation breakpoints for each watershed were identified based on antecedent water table elevation, streamflow, ROCs, and TSRs. These thresholds represent the groundwater elevation above which event runoff generation increased sharply in response to rainfall. For effective coastal land use decision making, baseline watershed hydrology must be understood to serve as a benchmark for management goals, based on both seasonal and event-based surface and groundwater interactions. C1 [Epps, Thomas H.; Loflin, Drake R.] Clemson Univ, Biosyst Engn Program, Georgetown, SC 29442 USA. [Hitchcock, Daniel R.; Jayakaran, Anand D.] Clemson Univ, Sch Agr Forest & Environm Sci, Baruch Inst Coastal Ecol & Forest Sci, Georgetown, SC 29442 USA. [Amatya, Devendra M.] US Forest Serv, USDA, Ctr Forested Wetlands Res, Cordesville, SC 29434 USA. RP Epps, TH (reprint author), Clemson Univ, Biosyst Engn Program, Georgetown, SC 29442 USA. EM dhitchc@clemson.edu RI Jayakaran, Anand/M-2441-2016 OI Jayakaran, Anand/0000-0003-2605-9759 FU South Carolina Sea Grant Consortium; NOAA [NA10OAR4I70073]; Clemson University Public Service Activities (PSA); NIFA/USDA under Clemson University Experiment Station [SC-1700393, 6055] FX The authors wish to thank Andy Harrison, Hydrologic Technician at the Santee Experimental Forest, USDA Forest Service, for assisting with data management and sharing. We also wish to thank our collaborators on the larger aspects of this project, notably Dr. Tim Callahan and Dr. Vijay Vulava of the College of Charleston, as well as their very talented graduate students Guinn Garrett and Michael Griffin. This journal article was prepared in part as a result of work sponsored by the South Carolina Sea Grant Consortium with NOAA financial assistance award No. NA10OAR4I70073. This work was also supported by Clemson University Public Service Activities (PSA) through the Intelligent River (TM) project. This material is also based upon work supported by NIFA/USDA, under project number SC-1700393, Technical Contribution No. 6055 of the Clemson University Experiment Station. NR 28 TC 3 Z9 3 U1 2 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD FEB PY 2013 VL 49 IS 1 BP 76 EP 89 DI 10.1111/jawr.12000 PG 14 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 087IU UT WOS:000314755700006 ER PT J AU Boggs, J Sun, G Jones, D McNulty, SG AF Boggs, Johnny Sun, Ge Jones, David McNulty, Steven G. TI Effect of Soils on Water Quantity and Quality in Piedmont Forested Headwater Watersheds of North Carolina SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE Triassic Basins; Carolina Slate Belt; forest hydrology; streamflow; water quality; North Carolina Piedmont ID SOUTHEASTERN UNITED-STATES; CATCHMENT; NITROGEN; STREAMS; TOPOGRAPHY; RETENTION; HYDROLOGY; RESPONSES; IMPACTS; RUNOFF AB Boggs, Johnny, Ge Sun, David Jones, and Steven G. McNulty, 2012. Effect of Soils on Water Quantity and Quality in Piedmont Forested Headwater Watersheds of North Carolina. Journal of the American Water Resources Association (JAWRA) 1-19. DOI: 10.1111/jawr.12001 Abstract: Water quantity and quality data were compared from six headwater watersheds on two distinct soil formations, Carolina Slate Belt (CSB) and Triassic Basins (TB). CSB soils are generally thicker, less erodible, and contain less clay content than soils found in TB. TB generated significantly more discharge/precipitation ratio than CSB (0.33 vs. 0.24) in the 2009 dormant season. In the 2009 growing season, TB generated significantly less discharge/precipitation ratio than CSB (0.02 vs. 0.07). Over the entire monitoring period, differences in discharge/precipitation ratios between CSB and TB were not significantly different (0.17 vs. 0.20, respectively). Storm-flow rates were significantly higher in TB than CSB in both dormant and growing season. Benthic macroinvertebrate biotic index scores were excellent for all streams. Nutrient concentrations and exports in CSB and TB were within background levels for forests. Low-stream nitrate and ammonium concentrations and exports suggested that both CSB and TB were nitrogen limited. Soils appear to have had a significant influence on seasonal and storm-flow generation, but not on long-term total water yield and water quality under forested conditions. This study indicated that watersheds on TB soils might be more prone to storm-flow generation than on CSB soils when converted from forest to urban. Future urban growth in the area should consider differences in baseline hydrology and effects of landuse change on water quantity and quality. C1 [Boggs, Johnny; Sun, Ge; McNulty, Steven G.] US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. [Jones, David] NCDA Forest Serv, Raleigh, NC 27699 USA. RP Boggs, J (reprint author), US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, 920 Main Campus Dr Suite 300, Raleigh, NC 27606 USA. EM jboggs@ncsu.edu FU NC Department of Environment and Natural Resources Division of Water Quality; US Forest Service Eastern Forest Environmental Threat Assessment Center; USEPA FX This research was funded by the USEPA Non-Point Source (NPS) Pollution Control Grant through Section 319(h) of the Clean Water Act, NC Department of Environment and Natural Resources Division of Water Quality and US Forest Service Eastern Forest Environmental Threat Assessment Center. We would like to thank Jennifer Moore Myers for N deposition kriging analysis, and students and support staff for their diligent work in the field and laboratory. We would also like to especially thank William "Bill'' Swartley and Tom Gerow from NC Forest Service for their project support and guidance. NR 77 TC 6 Z9 6 U1 4 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD FEB PY 2013 VL 49 IS 1 BP 132 EP 150 DI 10.1111/jawr.12001 PG 19 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 087IU UT WOS:000314755700010 ER PT J AU Stewart, LR Haque, MA Jones, MW Redinbaugh, MG AF Stewart, Lucy R. Haque, Md. Ashraful Jones, Mark W. Redinbaugh, Margaret G. TI Response of maize (Zea mays L.) lines carrying Wsm1, Wsm2, and Wsm3 to the potyviruses Johnsongrass mosaic virus and Sorghum mosaic virus SO MOLECULAR BREEDING LA English DT Article DE Potyvirus; JGMV; SrMV; Virus resistance; Wsm; Maize ID LOCI CONTROLLING RESISTANCE; COAT PROTEIN; ARABIDOPSIS-THALIANA; SWEET CORN; RT-PCR; SUGARCANE; DWARF; STRAIN; IDENTIFICATION; GENE AB Maize dwarf mosaic disease is one of the most important viral diseases of maize (Zea mays L.) throughout the world. It is caused by several virus species in the family Potyviridae, genus Potyvirus, including Maize dwarf mosaic virus (MDMV), Sugarcane mosaic virus (SCMV), Johnsongrass mosaic virus (JGMV) and Sorghum mosaic virus (SrMV). Resistance to another member of the family Potyviridae, Wheat streak mosaic virus (WSMV), is conferred by three alleles (Wsm1, Wsm2, Wsm3) in the maize inbred line Pa405, and these or closely linked genes were previously shown to confer resistance to the potyviruses MDMV and SCMV. In this study, we assessed whether Wsm alleles are linked to resistance to JGMV and SrMV. Near isogenic lines (NILs) carrying one or two of the Wsm alleles introgressed into the susceptible line Oh28 and F1 progeny from NIL x Oh28 were tested for their response to JGMV and SrMV. Our results indicate that Wsm1 provides resistance to both JGMV and SrMV in a dose-dependent manner. Wsm2 and Wsm3 each provide limited resistance, and combining Wsm alleles enhances that resistance. C1 [Stewart, Lucy R.; Jones, Mark W.; Redinbaugh, Margaret G.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Wooster, OH 44691 USA. [Stewart, Lucy R.; Haque, Md. Ashraful; Redinbaugh, Margaret G.] OSU OARDC, Dept Plant Pathol, Wooster, OH USA. RP Stewart, LR (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Wooster, OH 44691 USA. EM lucy.stewart@ars.usda.gov RI Redinbaugh, Margaret/A-3611-2013 FU Islamic Development Bank (IDB) FX We thank Islamic Development Bank (IDB) for providing financial support for Dr. Md. A. Haque. We also thank Kristen Willie (USDA-ARS) and Christopher Nacci (USDA-ARS) for expert technical assistance. NR 44 TC 5 Z9 5 U1 2 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2013 VL 31 IS 2 BP 289 EP 297 DI 10.1007/s11032-012-9789-5 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 086VO UT WOS:000314716700004 ER PT J AU Glynn, NC Laborde, C Davidson, RW Irey, MS Glaz, B D'Hont, A Comstock, JC AF Glynn, Neil C. Laborde, Chris Davidson, R. Wayne Irey, Mike S. Glaz, Barry D'Hont, Angelique Comstock, Jack C. TI Utilization of a major brown rust resistance gene in sugarcane breeding SO MOLECULAR BREEDING LA English DT Article DE Sugarcane (Saccharum spp.); Brown rust; Puccinia melanocephala; Marker-assisted selection ID BRU1; MELANOCEPHALA; LOUISIANA AB Brown rust, caused by Puccinia melanocephala, has had devastating effects on sugarcane (Saccharum spp.) breeding programs and commercial production. The discovery of Bru1, a major gene conferring resistance to brown rust, represented a substantial breakthrough. Markers for Bru1 are the first available for sugarcane molecular breeding. The contribution of Bru1 towards brown rust resistance in the Canal Point (CP) sugarcane breeding program was determined as a means of directing future breeding strategies. Bru1 was detected in 285 of 1,072 (27 %) clones used for crossing; this germplasm represents the genetic base for cultivar development in Florida. The frequency of Bru1 was greatest in CP clones (42 %) and lowest among Louisiana clones (6 %). Bru1 was not detected in clones with year assignments before 1953. However, Bru1 frequency increased from 15 % (assignments 1975-1985) to 47 % in the current decade. The increase coincided with the introduction of brown rust to Florida. Bru1 was detected in 155 (32 %) of 485 parental clones tested for brown rust susceptibility at two field locations. Of clones classed resistant to brown rust, 154 (59 %) contained Bru1, yet none of 100 susceptible clones contained the gene. Bru1 was detected in 667 (44 %) clones in the second clonal stage of selection, 87 % of which were free of brown rust symptoms. Bru1 is the predominant source of resistance in the Florida sugarcane genetic base. Efforts to identify and integrate new brown rust resistance genes must be pursued to minimize risks associated with a future breakdown in major gene resistance provided by Bru1. C1 [Glynn, Neil C.; Glaz, Barry; Comstock, Jack C.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Laborde, Chris] Okeelanta Sugar Mill, Florida Crystals, S Bay, FL 33493 USA. [Davidson, R. Wayne] Florida Sugar Cane League Inc, Clewiston, FL 33440 USA. [Irey, Mike S.] US Sugar Corp, Clewiston, FL 33440 USA. [D'Hont, Angelique] CIRAD, UMR AGAP, Montpellier, France. RP Glynn, NC (reprint author), ARS, USDA, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. EM neilglynn@gmail.com NR 25 TC 12 Z9 17 U1 0 U2 31 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2013 VL 31 IS 2 BP 323 EP 331 DI 10.1007/s11032-012-9792-x PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 086VO UT WOS:000314716700007 ER PT J AU Daneshgar, PP Polley, HW Wilsey, BJ AF Daneshgar, Pedram P. Polley, H. Wayne Wilsey, Brian J. TI Simple plant traits explain functional group diversity decline in novel grassland communities of Texas SO PLANT ECOLOGY LA English DT Article DE Novel ecosystems; Biodiversity; Invasive species; Altered rainfall; Functional diversity; Eragrostis curvula; Panicum coloratum; Sorghum halepense ID TALLGRASS PRAIRIE; MORPHOLOGICAL TRAITS; INTRODUCED GRASS; INVASIVE PLANTS; NATIVE GRASSES; USE EFFICIENCY; EXOTIC PLANTS; RESOURCE-USE; PRODUCTIVITY; BIODIVERSITY AB Previous research has found that plant diversity declines more quickly in exotic than native grassland plots, which offers a model system for testing whether diversity decline is associated with specific plant traits. In a common garden experiment in the Southern Great Plains in central Texas, USA, we studied monocultures and 9-species mixtures of either all exotic or all native grassland species. A total of 36 native and exotic species were paired by phylogeny and functional group. We used community-level measures (relative abundance in mixture) and whole-plant (height, aboveground biomass, and light capture) and leaf-level traits (area, specific leaf area, and C:N ratio) to determine whether trait differences explained native-exotic differences in functional group diversity. Increases in species' relative abundance in mixture were correlated with high biomass, height, and light capture in both native and exotic communities. However, increasing exotic species were all C-4 grasses, whereas, increasing native species included forb, C-3 grass and C-4 grass species. Exotic C-4 grasses had traits associated with relatively high resource capture: greater leaf area, specific leaf area, height, biomass, and light capture, but similar leaf C:N ratios compared to native C-4 grasses. Leaf C:N was consistently higher for native than exotic C-3 species, implying that resource use efficiency was greater in natives than exotics. Our results suggest that functional diversity will differ between grasslands restored to native assemblages and those dominated by novel collections of exotic species, and that simple plant traits can help to explain diversity decline. C1 [Daneshgar, Pedram P.] Monmouth Univ, Dept Biol, New York, NJ 07764 USA. [Polley, H. Wayne] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Wilsey, Brian J.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. RP Wilsey, BJ (reprint author), Iowa State Univ, Dept Ecol Evolut & Organismal Biol, 253 Bessey Hall, Ames, IA 50011 USA. EM bwilsey@iastate.edu OI Wilsey, Brian J./0000-0002-0628-5006 FU National Science Foundation [DEB0639417] FX We thank the following individuals for their help with planting, weeding, sampling, and harvesting: Tim Dickson, Phil Fay, Anne Gibson, Forest Isbell, Katherine Jones, Parker Knutson, Chris Kolodziejczyk, Alicia Naranjo, Terri Beth Teaschner, Kyle Tiner, and Anna Loan-Wilsey. Two anonymous reviewers supplied helpful comments on an earlier version of this manuscript. Funding was provided by a grant from the National Science Foundation (DEB0639417) to BW. NR 50 TC 3 Z9 3 U1 5 U2 88 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD FEB PY 2013 VL 214 IS 2 BP 231 EP 241 DI 10.1007/s11258-012-0162-x PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 087SB UT WOS:000314781600006 ER PT J AU Lang, M McCarty, G Oesterling, R Yeo, IY AF Lang, Megan McCarty, Greg Oesterling, Robert Yeo, In-Young TI Topographic Metrics for Improved Mapping of Forested Wetlands SO WETLANDS LA English DT Article DE Hydroperiod; Hydropattern; Inundation; Relief; Topographic wetness index; Wetland mapping ID DIGITAL ELEVATION MODELS; WETNESS INDEX; INVENTORY MAPS; LIDAR; ALGORITHMS; PATTERNS; STATES; AREAS AB We investigated the predictive strength of forested wetland maps produced using digital elevation models (DEMs) derived from Light Detection and Ranging (LiDAR) data and multiple topographic metrics, including multiple topographic wetness indices (TWIs), a TWI enhanced to incorporate information on water outlets, normalized relief, and hybrid TWI/relief in the Coastal Plain of Maryland. LiDAR DEM based wetland maps were compared to maps of inundation and existing wetland maps. TWIs based on the most distributed FD8 (8 cells) and somewhat distributed Da (1-2 cells) flow routing algorithms were better correlated with inundation than a TWI based on a non-distributed D8 (1 cell) flow routing algorithm, but Da TWI class boundaries appeared artificial. The enhanced FD8 TWI provided good prediction of wetland location but could not predict periodicity of inundation. Normalized relief provided good prediction of inundation periodicity but was less able to map wetland boundaries. A hybrid of these metrics provided good measurement of wetland location and inundation periodicity. Wetland maps based on topographic metrics included areas of flooded forest that were similar to an aerial photography based wetland map. These results indicate that LiDAR based topographic metrics have potential to improve accuracy and automation of wetland mapping. C1 [Lang, Megan] Forest Serv, USDA, No Res Stn, Beltsville, MD 20705 USA. [McCarty, Greg] Agr Res Serv, USDA, Beltsville Agr Res Ctr, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Oesterling, Robert; Yeo, In-Young] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. RP Lang, M (reprint author), Forest Serv, USDA, No Res Stn, 10300 Baltimore Ave,Bldg 007,Rm 104, Beltsville, MD 20705 USA. EM mwlang@fs.fed.us FU wetland component of the Natural Resources Conservation Service Conservation Effects Assessment Project FX This research was supported by the wetland component of the Natural Resources Conservation Service Conservation Effects Assessment Project. We greatly acknowledge the cooperation of private landowners, especially The Nature Conservancy. All trade names are included for the benefit of the reader and do not imply an endorsement of or preference for the product listed by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 38 TC 18 Z9 19 U1 4 U2 42 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD FEB PY 2013 VL 33 IS 1 BP 141 EP 155 DI 10.1007/s13157-012-0359-8 PG 15 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 086ZF UT WOS:000314728600011 ER PT J AU Kunkel, KE Atwood, TC Ruth, TK Pletscher, DH Hornocker, MG AF Kunkel, K. E. Atwood, T. C. Ruth, T. K. Pletscher, D. H. Hornocker, M. G. TI Assessing wolves and cougars as conservation surrogates SO ANIMAL CONSERVATION LA English DT Article DE conservation surrogate; cougar; focal species; landscape; wolves ID RESOURCE SELECTION FUNCTIONS; GLACIER-NATIONAL-PARK; PREDATOR-PREY SYSTEM; HABITAT SELECTION; LOGISTIC-REGRESSION; POPULATION-DYNAMICS; TOP PREDATORS; MONTANA; RISK; WINTER AB Large carnivores have been posited as potential conservation surrogates to inform the design and prioritization of conservation planning. We show that wolves Canis lupus and cougars Puma concolor may have potential to serve as a surrogate suite for conserving landscape heterogeneity, hypothesized to be a determinant of biodiversity in some landscapes. We examined habitat and landscape features associated with the spatial distribution of wolf- and cougar-killed prey in the basin of the North Fork of the Flathead River in Montana. The spatial distribution of wolf-killed prey was driven largely by cover type, whereas physiographic characteristics were the primary driver of the distribution of cougar-killed prey. Spatial templates, generated using >0.66 probability quantiles from spatially explicit models of kill site distribution, estimated over three times as much high-quality habitat for wolves (1005?km2) than for cougars (381?km2). While there were only minor differences in the proportional representation of land cover types between the wolf and cougar templates, 40% of the cougar template fell outside the wolf template, and the former contained over three times more rugged terrain than the latter. The use of a combined wolfcougar spatial template resulted in a 15% increase in total area and 91% increase in the amount of rugged terrain identified. Based on our models, the advantage of using both wolves and cougars as a focal suite in north-west Montana is the ability to identify a greater area of high-quality habitat, and capture landscape heterogeneity that may be important to conserving biodiversity. C1 [Kunkel, K. E.; Pletscher, D. H.] Univ Montana, Sch Forestry, Wildlife Biol Program, Missoula, MT 59812 USA. [Atwood, T. C.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Ruth, T. K.; Hornocker, M. G.] Wildlife Conservat Soc, Bozeman, MT USA. RP Kunkel, KE (reprint author), Univ Montana, Sch Forestry, Wildlife Biol Program, Missoula, MT 59812 USA. EM kyran@montana.net FU U.S. Fish and Wildlife Service; National Park Service; U.S. Forest Service; British Columbia Ministry of Environment; Montana Department of Fish, Wildlife and Parks; McIntire-Stennis Program at the School of Forestry, University of Montana; Peter W. Busch Family Foundation; Richard King Mellon Foundation; National Geographic Society FX We appreciate financial support from the U.S. Fish and Wildlife Service; National Park Service; U.S. Forest Service; British Columbia Ministry of Environment; Montana Department of Fish, Wildlife and Parks; McIntire-Stennis Program at the School of Forestry, University of Montana; Peter W. Busch Family Foundation; Richard King Mellon Foundation; G.C. Hixon; and the National Geographic Society. We thank W. Clark, S. Emmerich, R. Altop, H. Quigley, R. Ream, D. Boyd, E. Bangs, S. Gniadek, H. Nyberg, W. Arjo, J. Fontaine, S. Fritts, and A. Fontana for valuable advice and field support. D. Hoerner of Eagle Aviation served as an exceptional pilot and provided countless hours of safe flights. We especially thank field technicians J. Altermatt, V. Asher, S. Cooper, G. Dicus, B. French, D. Gigneaux, C. Gray, J. Jonkel, D. Kauferle, S. Lewis, K. Lohr, W. Lowe, T. Parker, J. Putera, M. Rohweder, R. Siemans, J. Terenzi, R. Vinkey, and A. Whitelaw. J. Doskocil and P. Singleton aided in GIS analysis. NR 55 TC 9 Z9 9 U1 1 U2 130 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1367-9430 J9 ANIM CONSERV JI Anim. Conserv. PD FEB PY 2013 VL 16 IS 1 BP 32 EP 40 DI 10.1111/j.1469-1795.2012.00568.x PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 079LJ UT WOS:000314171600006 ER PT J AU Ruberson, JR Takasu, K Buntin, GD Eger, JE Gardner, WA Greene, JK Jenkins, TM Jones, WA Olson, DM Roberts, PM Suiter, DR Toews, MD AF Ruberson, John R. Takasu, Keiji Buntin, G. David Eger, Joe E., Jr. Gardner, Wayne A. Greene, Jeremy K. Jenkins, Tracie M. Jones, Walker A. Olson, Dawn M. Roberts, Phillip M. Suiter, Daniel R. Toews, Michael D. TI From Asian curiosity to eruptive American pest: Megacopta cribraria (Hemiptera: Plataspidae) and prospects for its biological control SO APPLIED ENTOMOLOGY AND ZOOLOGY LA English DT Review DE Kudzu bug; Bean plataspid; Globular stink bug; Biological control; Invasive species ID PRELIMINARY HOST-RANGE; CONTROL AGENTS; HYMENOPTERA; HETEROPTERA; SCELIONIDAE; APHELINIDAE; ENCYRTIDAE; REVISION; INSECT; GROWTH AB The kudzu bug or bean plataspid, Megacopta cribraria (Fabricius), is native to Asia where it appears to be widely distributed (although the taxonomy is not entirely clear), but is infrequently a pest of legumes. This bug appeared in 2009 in the southeastern United States, where it is closely associated with kudzu, Pueraria montana Lour. [Merr.] variety lobata [Willd.] Maesen & S. Almeida. However, the insect has become a consistent economic pest of soybeans, Glycine max (L.) Merr., and some other leguminous crops in areas where large numbers can build in kudzu, in addition to being a considerable nuisance in urban landscapes where kudzu occurs. The insect has remarkable capacity for movement and has spread rapidly from nine Georgia counties in 2009 to seven states in 2012. Despite being a nuisance in urban areas and a crop pest, high populations of the bug also reduce the biomass of kudzu, which is itself a seriously problematic invasive weed, complicating the status of M. cribraria in its expanded range. Extant predators and a pathogen in the US have been observed attacking kudzu bugs in the laboratory and field, but no parasitism of eggs or nymphs has been observed to date. A single record of parasitism of an adult kudzu bug by a tachinid fly is known from the US, but no other adult parasitism has been observed in the US or elsewhere. Extant enemies may eventually significantly reduce the bug's populations, but at present native enemies appear to be insufficient for the task, and exotic enemies from the kudzu bug's native range may offer the best possibility for effective biological control in the US. Based on the available literature, the best option for an importation biological control program appears to be the platygastrid egg parasitoid Paratelenomus saccharalis (Dodd) because of its apparent host specificity, intimate biological linkages with M. cribraria, and wide geographic distribution in the Eastern Hemisphere. Other natural enemies may eventually emerge as good candidates for importation, but at present P. saccharalis appears to be the most promising. C1 [Ruberson, John R.; Roberts, Phillip M.; Toews, Michael D.] Univ Georgia, Dept Entomol, Tifton, GA 31793 USA. [Takasu, Keiji] Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan. [Buntin, G. David; Gardner, Wayne A.; Jenkins, Tracie M.; Suiter, Daniel R.] Univ Georgia, Dept Entomol, Griffin, GA 30223 USA. [Eger, Joe E., Jr.] Dow AgroSci, Tampa, FL 33629 USA. [Greene, Jeremy K.] Clemson Univ, Edisto Res & Educ Ctr, Sch Agr Forest & Environm Sci, Blackville, SC 29817 USA. [Jones, Walker A.] ARS, Biol Control Pests & Natl Biol Control Lab, USDA, Stoneville, MS 38776 USA. [Olson, Dawn M.] ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA 31793 USA. RP Ruberson, JR (reprint author), Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. EM ruberson@ksu.edu RI U-ID, Kyushu/C-5291-2016; OI Ruberson, John/0000-0002-4475-8177 NR 50 TC 28 Z9 29 U1 6 U2 106 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 0003-6862 J9 APPL ENTOMOL ZOOL JI Appl. Entomol. Zoolog. PD FEB PY 2013 VL 48 IS 1 BP 3 EP 13 DI 10.1007/s13355-012-0146-2 PG 11 WC Entomology SC Entomology GA 087FP UT WOS:000314747000002 ER PT J AU Burki, R Krasnov, A Bettge, K Rexroad, CE Afanasyev, S Antikainen, M Burkhardt-Holm, P Wahli, T Segner, H AF Burki, Richard Krasnov, Aleksei Bettge, Kathrin Rexroad, Caird E., III Afanasyev, Sergey Antikainen, Miia Burkhardt-Holm, Patricia Wahli, Thomas Segner, Helmut TI Molecular crosstalk between a chemical and a biological stressor and consequences on disease manifestation in rainbow trout SO AQUATIC TOXICOLOGY LA English DT Article DE Pathogen; Proliferative kidney disease; Estrogen; Endocrine disruption; Multiple stressors; Rainbow trout ID PROLIFERATIVE KIDNEY-DISEASE; GENE-EXPRESSION; RISK-ASSESSMENT; ONCORHYNCHUS-MYKISS; MULTIPLE STRESSORS; BROWN TROUT; PESTICIDE EXPOSURE; SALMO-TRUTTA; RESPONSES; TEMPERATURE AB The aim of the present study was to examine the molecular and organism reaction of rainbow trout, Oncorhynchus mykiss, to the combined impact of two environmental stressors. The two stressors were the myxozoan parasite, Tetracapsuloides bryosalmonae, which is the etiological agent of proliferative kidney disease (PKD) and a natural stressor to salmonid populations, and 17 beta-estradiol (E2) as prototype of estrogen-active chemical stressors in the aquatic environment. Both stressors, the parasite and estrogenic contaminants, co-exist in Swiss rivers and are discussed as factors contributing to the decline of Swiss brown trout populations over the last decades. Using a microarray approach contrasting parasite-infected and non-infected rainbow trout at low or high estrogen levels, it was observed that molecular response patterns under joint exposure differed from those to the single stressors. More specifically, three major response patterns were present: (i) expression responses of gene transcripts to one stressor are weakened by the presence of the second stressor; (ii) expression responses of gene transcripts to one stressor are enhanced by the presence of the second stressor; (iii) expression responses of gene transcripts at joint treatment are dominated by one of the two stressors. Organism-level responses to concurrent E2 and parasite treatment - assessed through measuring parasite loads in the fish host and cumulative mortalities of trout - were dominated by the pathogen, with no modulating influence of E2. The findings reveal function- and level-specific responses of rainbow trout to stressor combinations, which are only partly predictable from the response to the single stressors. (c) 2012 Elsevier B.V. All rights reserved. C1 [Burki, Richard; Bettge, Kathrin; Wahli, Thomas; Segner, Helmut] Univ Bern, Ctr Fish & Wildlife Hlth, CH-3001 Bern, Switzerland. [Krasnov, Aleksei] NOFIMA Marin, N-1432 As, Norway. [Rexroad, Caird E., III] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Afanasyev, Sergey] IM Sechenov Evolutionary Physiol & Biochem Inst, Peterburg 194223, Russia. [Antikainen, Miia] Univ Kuopio, Inst Appl Biotechnol, FIN-70211 Kuopio, Finland. [Burkhardt-Holm, Patricia] Univ Basel, Man Soc Environm, CH-4051 Basel, Switzerland. RP Segner, H (reprint author), Univ Bern, Ctr Fish & Wildlife Hlth, Langgassstr 122, CH-3001 Bern, Switzerland. EM helmut.segner@vetsuisse.unibe.ch RI Krasnov, Aleksei/D-3065-2012; Segner, Helmut/D-5714-2014; Afanasyev, Sergey/K-9309-2015 OI Segner, Helmut/0000-0002-1783-1295; Afanasyev, Sergey/0000-0002-8393-820X FU Swiss Nationalfunds [4050-066568] FX The financial support of Swiss Nationalfunds (project No 4050-066568) is gratefully acknowledged. We also thank U. Sattler (University Bern) for excellent technical assistance in performing real-time RT-PCR. Turku Centre for Biotechnology, Finland is acknowledged for preparation of microarrays. NR 58 TC 5 Z9 5 U1 0 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-445X J9 AQUAT TOXICOL JI Aquat. Toxicol. PD FEB PY 2013 VL 127 SI SI BP 2 EP 8 DI 10.1016/j.aquatox.2012.02.026 PG 7 WC Marine & Freshwater Biology; Toxicology SC Marine & Freshwater Biology; Toxicology GA 085OC UT WOS:000314623100002 PM 22440717 ER PT J AU Paretas-Martinez, J Forshage, M Buffington, M Fisher, N La Salle, J Pujade-Villar, J AF Paretas-Martinez, Jordi Forshage, Mattias Buffington, Matthew Fisher, Nicole La Salle, John Pujade-Villar, Juli TI Overview of Australian Cynipoidea (Hymenoptera) SO AUSTRALIAN JOURNAL OF ENTOMOLOGY LA English DT Article DE Australia; Austrocynipidae; Cynipidae; Figitidae; Ibaliidae; Liopteridae ID FAMILY IBALIIDAE HYMENOPTERA; SP N. HYMENOPTERA; OAK GALL WASPS; EUCOILINAE HYMENOPTERA; CHARIPINAE HYMENOPTERA; FIGITIDAE HYMENOPTERA; FORSTER HYMENOPTERA; BIOLOGICAL-CONTROL; PUJADE-VILLAR; SOUTH-AFRICA AB An overview of all families, subfamilies, genera and species of Cynipoidea present in Australia is presented. The Australian cynipoid fauna is very poorly known, with 37 genera recorded: one each for Austrocynipidae, Ibaliidae and Liopteridae; two for Cynipidae; and 32 for Figitidae. The first Australian records are given for the following genera of Eucoilinae: Aganaspis Lin, Areaspis Lin, Chrestosema Forster, Didyctium Riley, Endecameris Yoshimoto, Ganaspis Forster, Leptolamina Yoshimoto, Micreriodes Yoshimoto, Pseudodiranchis Yoshimoto, Sinochresta Lin and Weldia Yoshimoto. Nine new combinations, two new synonymies and one reinstatement are made: Eucoilinae (Figitidae): Hexacola aemilia comb. n., Hexacola florentia comb. n., Hexacola julia comb. n., Hexacola mozarti comb. n., Hexacola thoreauini comb. n., Kleidotoma marguerita comb. n., Leptopilina lonchaeae comb. n., Leptopilina maria comb. n., Trybliographa australiensis stat. rev. (Rhoptromeris unimaculus syn. n.); Thrasorinae (Figitidae): Thrasorus berlesi comb. n. (Thrasorus rieki Paretas-Martinez & Pujade-Villar 2011 syn. n.). Aspects on the systematics, distribution, biology and morphology of all cynipoid families and figitid subfamilies in Australia are given. A multi-character online key to the genera of Australian Cynipoidea can be found at http://www.ces.csiro.au/keys/Hymenoptera/Australian_Cynipoidea/Australian-Cynipoidea-Keys.html. C1 [Paretas-Martinez, Jordi; Pujade-Villar, Juli] Univ Barcelona, Fac Biol, Dept Anim Biol, E-08028 Barcelona, Spain. [Forshage, Mattias] Swedish Museum Nat Hist, Dept Entomol, SE-10405 Stockholm, Sweden. [Buffington, Matthew] NMNH, Systemat Entomol Lab, USDA, Smithsonian Inst, Washington, DC 20013 USA. [Fisher, Nicole] CSIRO Ecosyst Sci, Australian Natl Insect Collect, Canberra, ACT 2601, Australia. [La Salle, John] CSIRO Ecosyst Sci, Atlas Living Australia, Canberra, ACT 2601, Australia. RP Paretas-Martinez, J (reprint author), Univ Barcelona, Fac Biol, Dept Anim Biol, Avda Diagonal 645, E-08028 Barcelona, Spain. EM jordi.paretas.martinez@gmail.com RI La Salle, John/B-9544-2008; Fisher, Nicole/B-3616-2009; Pujade-Villar, Juli/K-9838-2014 OI La Salle, John/0000-0002-8816-9569; NR 89 TC 5 Z9 6 U1 1 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1326-6756 EI 1440-6055 J9 AUST J ENTOMOL JI Aust. J. Entomol. PD FEB PY 2013 VL 52 IS 1 BP 73 EP 86 DI 10.1111/j.1440-6055.2012.00877.x PG 14 WC Entomology SC Entomology GA 084LN UT WOS:000314540900010 ER PT J AU Guy, ZM Birkeland, KW AF Guy, Zachary M. Birkeland, Karl W. TI Relating complex terrain to potential avalanche trigger locations SO COLD REGIONS SCIENCE AND TECHNOLOGY LA English DT Article DE Avalanche; Spatial variability; Terrain parameters; Couloirs; Persistent weak layers ID SPATIAL VARIABILITY; SURFACE HOAR; SNOW STABILITY; RESISTANCE; CRYSTALS; PATTERNS; SLOPES; LAYERS AB More winter recreationists are venturing into "extreme" terrain each year, and avalanche fatalities in that terrain are increasing. The slope-scale spatial variability of snow stability and how it relates to this complex terrain is critically important but poorly understood. In this study, we use terrain parameters to model potential trigger locations (PTLs) of slab avalanches, which are defined based on a minimum slab thickness overlying a persistent weak layer or the presence of a weak layer on the snow surface which could be subsequently buried. In a sample of seventeen couloirs from Lone Mountain, Montana, field teams tracked and mapped persistent weak layers and slabs with probe and pit sampling. We used terrain parameters derived from a one-meter digital elevation model to explore the relationships between PTLs and terrain, and our results show strong statistical relationships exist. However, results varied widely from couloir to couloir, suggesting that the relationships between terrain and PTLs in each couloir are unique and highly complex. For these steep alpine couloirs, parameters relating to wind deposition, wind scouring, and stuffing are most strongly associated with PTLs. The influences of these and other terrain parameters vary, depending on broader-scale terrain characteristics, prior weather patterns, and seasonal trends. With an understanding of the broader scale influences and physical processes involved, we can use terrain to optimize stability test locations, explosive placements, or route selection. The unique nature of each couloir means that simple rules relating terrain to PTLs will not apply, although couloirs in the same cirque generally share similarities. This study will help to improve practical decision-making as well as future modeling efforts. (C) 2012 Elsevier B.V. All rights reserved. C1 [Guy, Zachary M.; Birkeland, Karl W.] Montana State Univ, Dept Earth Sci, Bozeman, MT 59717 USA. [Birkeland, Karl W.] US Forest Serv, USDA, Natl Avalanche Ctr, Bozeman, MT 59771 USA. RP Guy, ZM (reprint author), 680 Highview Ct, Estes Pk, CO 80517 USA. EM zach.guy@gmail.com FU U.S. National Science Foundation (BCS) [0518429]; American Avalanche Association; Mazamas; American Alpine Club; Association of American Geographers; Montana State University Earth Sciences Department FX We would like to thank Steve Custer and Stuart Challender for their insightful contributions throughout this research and in writing this paper. We would also like to thank our field assistants, as well as Jordy Hendrix, Dianna Cooksey, Lucy Marshall, and Jim Robison-Cox for their contributions. Big Sky and Moonlight Basin Ski patrols were instrumental in allowing access to terrain and logistical support, and LiDAR data was provided by Brian McGlynn and the MSU Watershed Hydrology Laboratory with support from the U.S. National Science Foundation (BCS #0518429). We are grateful for the funding support from the American Avalanche Association, Mazamas, the American Alpine Club, the Association of American Geographers, and the Montana State University Earth Sciences Department that made this research possible. NR 48 TC 4 Z9 4 U1 1 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-232X J9 COLD REG SCI TECHNOL JI Cold Reg. Sci. Tech. PD FEB PY 2013 VL 86 BP 1 EP 13 DI 10.1016/j.coldregions.2012.10.008 PG 13 WC Engineering, Environmental; Engineering, Civil; Geosciences, Multidisciplinary SC Engineering; Geology GA 084SD UT WOS:000314558500001 ER PT J AU Lee, X Azevedo, MD Armstrong, DJ Banowetz, GM Reimmann, C AF Lee, Xiaoyun Azevedo, Mark D. Armstrong, Donald J. Banowetz, Gary M. Reimmann, Cornelia TI The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor SO ENVIRONMENTAL MICROBIOLOGY REPORTS LA English DT Article ID FACTOR GAF; RHIZOSPHERE BACTERIA; EXOPRODUCT FORMATION; AMINO-ACIDS; GENE AB The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) shares biological activities with 4-formylaminooxyvinylglycine, a related molecule produced by Pseudomonas fluorescens WH6. We found that culture filtrates of a P.aeruginosa strain overproducing AMB weakly interfered with seed germination of the grassy weed Poa annua and strongly inhibited growth of Erwinia amylovora, the causal agent of the devastating orchard crop disease known as fire blight. AMB was active against a 4-formylaminooxyvinylglycine-resistant isolate of E.amylovora, suggesting that the molecular targets of the two oxyvinylglycines in Erwinia do not, or not entirely, overlap. The AMB biosynthesis and transport genes were shown to be organized in two separate transcriptional units, ambA and ambBCDE, which were successfully expressed from IPTG-inducible tac promoters in the heterologous host P.fluorescens CHA0. Engineered AMB production enabled this model biocontrol strain to become inhibitory against E.amylovora and to weakly interfere with the germination of several graminaceous seeds. We conclude that AMB production requires no additional genes besides ambABCDE and we speculate that their expression in marketed fire blight biocontrol strains could potentially contribute to disease control. C1 [Lee, Xiaoyun; Reimmann, Cornelia] Univ Lausanne, Dept Microbiol Fondamentale, Quartier UNIL Sorge, CH-1015 Lausanne, Switzerland. [Azevedo, Mark D.; Banowetz, Gary M.] USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR USA. [Armstrong, Donald J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Reimmann, C (reprint author), Univ Lausanne, Dept Microbiol Fondamentale, Quartier UNIL Sorge, Batiment Biophore, CH-1015 Lausanne, Switzerland. EM cornelia.reimmann@unil.ch FU USDA-CSREES; OSU Agricultural Research Foundation FX We thank Hugues Henry for help with AMB quantification and Dieter Haas for carefully reading the manuscript. At Oregon State University, support from the USDA-CSREES Grass Seed Cropping Systems for Sustainable Agriculture Special Grant Program and from the OSU Agricultural Research Foundation is gratefully acknowledged. The use of trade, firm or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 18 TC 6 Z9 7 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1758-2229 J9 ENV MICROBIOL REP JI Environ. Microbiol. Rep. PD FEB PY 2013 VL 5 IS 1 SI SI BP 83 EP 89 DI 10.1111/j.1758-2229.2012.00395.x PG 7 WC Environmental Sciences; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 083OO UT WOS:000314474500009 PM 23757135 ER PT J AU Harris, RA Nagy-Szakal, D Kellermayer, R AF Harris, R. Alan Nagy-Szakal, Dorottya Kellermayer, Richard TI Human metastable epiallele candidates link to common disorders SO EPIGENETICS LA English DT Article DE epigenetics; DNA methylation; nutrition; developmental origins; metastable epiallele ID DNA METHYLATION; PROMOTER METHYLATION; GENOME; CANCER; SUPPLEMENTATION; GLIOBLASTOMA; EPIGENETICS; EXPRESSION AB Metastable epialleles (MEs) are mammalian genomic loci where epigenetic patterning occurs before gastrulation in a stochastic fashion leading to systematic interindividual variation within one species. Importantly, periconceptual nutritional influences may modulate the establishment of epigenetic changes, such as DNA methylation at MEs. Based on these characteristics, we exploited Infinium HumanMethylation450 BeadChip kits in a 2-tissue parallel screen on peripheral blood leukocyte and colonic mucosal DNA from 10 children without identifiable large intestinal disease. This approach led to the delineation of 1776 CpG sites meeting our criteria for MEs, which associated with 1013 genes. The list of ME candidates exhibited overlaps with recently identified human genes (including CYP2E1 and MGMT, where methylation has been associated with Parkinson disease and glioblastoma, respectively) in which perinatal DNA methylation levels where linked to maternal periconceptual nutrition. One hundred 18 (11.6%) of the ME candidates overlapped with genes where DNA methylation correlated (r > 0.871; p < 0.055) with expression in the colon mucosa of 5 independent control children. Genes involved in homophilic cell adhesion (including cadherin-associated genes) and developmental processes were significantly overrepresented in association with MEs. Additional filtering of gene expression-correlated MEs defined 35 genes, associated with 2 or more CpG sites within a 10 kb genomic region, fulfilling the ME criteria. DNA methylation changes at a number of these genes have been linked to various forms of human disease, including cancers, such as asthma and acute myeloid leukemia (ALOX12), gastric cancer (EBF3), breast cancer (NAV1), colon cancer and acute lymphoid leukemia (KCNK15), Wilms tumor (protocadherin gene cluster; PCDHAs) and colorectal cancer (TCERG1L), suggesting a potential etiologic role for MEs in tumorigenesis and underscoring the possible developmental origins of these malignancies. The presented compendium of ME candidates may accelerate our understanding of the epigenetic origins of common human disorders. C1 [Harris, R. Alan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Nagy-Szakal, Dorottya; Kellermayer, Richard] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Sect Pediat Gastroenterol, Houston, TX 77030 USA. [Nagy-Szakal, Dorottya; Kellermayer, Richard] ARS, USDA, Childrens Nutr Res Ctr, Houston, TX USA. RP Kellermayer, R (reprint author), Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Sect Pediat Gastroenterol, Houston, TX 77030 USA. EM kellerma@bcm.edu FU NIDDK [DK56338] FX The authors would like to acknowledge the patients for providing samples, and all of our colleagues who contributed to the successful banking of the tissues. This study utilized the NIDDK supported Pediatric Inflammatory Bowel Disease Consortium Registry of the BaylorCollege of Medicine (DK56338; Texas Medical Center Digestive Disease Center (PI: Estes, MK) NR 33 TC 19 Z9 19 U1 1 U2 12 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1559-2294 J9 EPIGENETICS-US JI Epigenetics PD FEB PY 2013 VL 8 IS 2 BP 157 EP 163 DI 10.4161/epi.23438 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 086JQ UT WOS:000314680300004 PM 23321599 ER PT J AU Burkey, K Neufeld, H Chappelka, A Grantz, D AF Burkey, Kent Neufeld, Howie Chappelka, Art Grantz, David TI In memoriam SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Letter C1 [Burkey, Kent] ARS, Plant Sci Res Unit, USDA, Raleigh, NC USA. [Neufeld, Howie] Appalachian State Univ, Dept Biol, Boone, NC 28608 USA. [Chappelka, Art] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Grantz, David] Univ Calif, Kearney Agr Ctr, Parlier, CA USA. RP Burkey, K (reprint author), ARS, Plant Sci Res Unit, USDA, Raleigh, NC USA. EM neufeldhs@appstate.edu NR 1 TC 0 Z9 0 U1 1 U2 14 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2013 VL 11 IS 1 BP 12 EP 12 DI 10.1890/13.WB.003 PG 1 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 083RO UT WOS:000314483100014 ER PT J AU Hager, GW Belt, KT Stack, W Burgess, K Grove, JM Caplan, B Hardcastle, M Shelley, D Pickett, STA Groffman, PM AF Hager, Guy W. Belt, Kenneth T. Stack, William Burgess, Kimberly Grove, J. Morgan Caplan, Bess Hardcastle, Mary Shelley, Desiree Pickett, Steward T. A. Groffman, Peter M. TI Socioecological revitalization of an urban watershed SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID ECOLOGICAL-SYSTEMS; LAND-USE; ECOSYSTEMS; STREAM AB Older, economically troubled urban neighborhoods present multiple challenges to environmental quality. Here, we present results from an initiative in Baltimore, Maryland, where water-quality improvements were rooted in a socioecological framework that highlighted the interactions between biogeophysical dynamics and social actors and institutions. This framework led to implementation of best management practices followed by assessment of changes in human perception, behavior, and education programs. Results suggest that such an initiative can improve both water quality (eg reductions in nitrogen and phosphorus runoff) and quality of life (eg increased involvement in outdoor recreation by residents and improvements in student environmental literacy and performance) in urban neighborhoods. However, proposed solutions to the water-quality problems in such neighborhoods have (1) typically emphasized the need for stormwater facilities that are difficult to build and maintain and (2) comprehensively addressed neither the issues related to aging infrastructure and hydrologic complexity nor the benefits derived from linkages between resident perception of environmental improvements and behavior and water-quality outcomes. C1 [Hager, Guy W.; Hardcastle, Mary; Shelley, Desiree] Pk & People Fdn, Baltimore, MD USA. [Belt, Kenneth T.; Grove, J. Morgan] US Forest Serv, USDA, Baltimore, MD USA. [Stack, William] Ctr Watershed Protect, Ellicott City, MD USA. [Burgess, Kimberly] Baltimore Dept Publ Works, Bur Water & Wastewater, Surface Water Management Div, Baltimore, MD USA. [Caplan, Bess; Pickett, Steward T. A.; Groffman, Peter M.] Cary Inst Ecosyst Studies, Millbrook, NY USA. RP Hager, GW (reprint author), Pk & People Fdn, Baltimore, MD USA. EM groffmanp@caryinstitute.org FU National Science Foundation [DEB-0423476, BCS-0508054]; USFS Northern Research Station [WS263]; Baltimore City Department of Public Works; Chesapeake Bay Trust; Campbell Foundation; National Fish and Wildlife Foundation; Rauch Foundation; Maryland Department of Natural Resources; Chesapeake and Atlantic Coastal Bays Trust Fund FX Research in WS263 was supported by multiple sources, including National Science Foundation grants DEB-0423476 (Long Term Ecological Research) and BCS-0508054 (Biocomplexity in Coupled Natural Human Systems). The USFS Northern Research Station contributed support for the BES as well as targeted funding for the WS263 project. Other support was received from the Baltimore City Department of Public Works, Chesapeake Bay Trust, The Campbell Foundation, National Fish and Wildlife Foundation, the Rauch Foundation, Maryland Department of Natural Resources, and the Chesapeake and Atlantic Coastal Bays Trust Fund. NR 23 TC 12 Z9 12 U1 6 U2 99 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2013 VL 11 IS 1 BP 28 EP 36 DI 10.1890/120069 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 083RO UT WOS:000314483100017 ER PT J AU Hurteau, MD Hungate, BA Koch, GW North, MP Smith, GR AF Hurteau, Matthew D. Hungate, Bruce A. Koch, George W. North, Malcolm P. Smith, Gordon R. TI Aligning ecology and markets in the forest carbon cycle SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID SEQUESTRATION PROGRAM; TREE MORTALITY; CLIMATE-CHANGE; UNITED-STATES; LEAKY SINK; FIRE RISK; REDUCTION; EMISSIONS; STORAGE; ECOSYSTEMS AB A forest carbon (C) offset is a quantifiable unit of C that is commonly developed at the local or regional project scale and is designed to counterbalance anthropogenic C emissions by sequestering C in trees. In cap-and-trade programs, forest offsets have market value if the sequestered C is additional (more than would have occurred in the absence of the project) and permanent (sequestered within the project boundary for a specified period of time). Local management and ecological context determine the rate of C sequestration, risk of loss, and hence the market value. An understanding of global C dynamics can inform policy but may not be able to effectively price an ecosystem service, such as C sequestration. Appropriate pricing requires the assistance of ecologists to assess C stock abundance and stability over spatial and temporal scales appropriate for the regional market. We use the risk that sequestered C will be emitted as a result of wildfire (reversal risk) to show how ecological context can influence market valuation in offset programs. C1 [Hurteau, Matthew D.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Hungate, Bruce A.; Koch, George W.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. [North, Malcolm P.] US Forest Serv, Pacific SW Res Stn, USDA, Davis, CA USA. [Smith, Gordon R.] Ecofor LLC, Seattle, WA USA. RP Hurteau, MD (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. EM matthew.hurteau@psu.edu RI Hungate, Bruce/F-8991-2011; Hurteau, Matthew/D-2301-2009 OI Hungate, Bruce/0000-0002-7337-1887; Hurteau, Matthew/0000-0001-8457-8974 NR 39 TC 13 Z9 13 U1 3 U2 67 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2013 VL 11 IS 1 BP 37 EP 42 DI 10.1890/120039 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 083RO UT WOS:000314483100018 ER PT J AU Chettri, P Ehrlich, KC Cary, JW Collemare, J Cox, MP Griffiths, SA Olson, MA de Wit, PJGM Bradshaw, RE AF Chettri, Pranav Ehrlich, Kenneth C. Cary, Jeffrey W. Collemare, Jerome Cox, Murray P. Griffiths, Scott A. Olson, Malin A. de Wit, Pierre J. G. M. Bradshaw, Rosie E. TI Dothistromin genes at multiple separate loci are regulated by AflR SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Gene regulation; Dothistroma septosporum; Secondary metabolism; Aflatoxin; Gene cluster; Cladosporium fulvum ID ASPERGILLUS-PARASITICUS; AFLATOXIN BIOSYNTHESIS; FOREST PATHOGEN; FILAMENTOUS FUNGI; SECONDARY METABOLISM; CLUSTER PROTEIN; PATHWAY GENES; NEEDLE BLIGHT; PINI; EXPRESSION AB In fungi, genes involved in the production of secondary metabolites are generally clustered at one location. There are some exceptions, such as genes required for synthesis of dothistromin, a toxin that is a chemical analog of the aflatoxin precursor versicolorin A and made by the pine needle pathogen Dothistroma septosporum. The availability of the D. septosporum genome sequence enabled identification of putative dothistromin genes, including an ortholog of the aflatoxin regulatory gene AflR, and revealed that most of the genes are spread over six separate regions (loci) on chromosome 12 (1.3 Mb). Here we show that levels of expression of the widely dispersed genes in D. septosporum are not correlated with gene location with respect to their distance from a telomere, but that AflR regulates them. The production of dothistromin by D. septosporum in which the AflR gene was knocked out (Delta DsAflR) was drastically reduced, but still detectable. This is in contrast to orthologous Delta AflR mutants in Aspergillus species that lack any aflatoxin production. Expression patterns in Delta DsAflR mutants helped to predict the complete set of genes involved in dothistromin production. This included a short-chain aryl alcohol dehydrogenase (NorB), which is located on chromosome 11 rather than chromosome 12, but was 24-fold down regulated in Delta DsAflR. An orthologous set of dothistromin genes, organized in a similar fragmented cluster arrangement to that seen in D. septosporum, was found in the closely related tomato pathogen Cladosporium fulvum even though this species does not produce dothistromin. In C fulvum, pseudogenization of key biosynthetic genes explains the lack of dothistromin production. The fragmented arrangement of dothistromin genes provides an example of coordinated control of a dispersed set of secondary metabolite genes; it also provides an example where loss of dothistromin production might have allowed adaptation to a new pathogenic lifestyle. (C) 2012 Elsevier Inc. All rights reserved. C1 [Chettri, Pranav; Cox, Murray P.; Olson, Malin A.; Bradshaw, Rosie E.] Massey Univ, Inst Mol BioSci, Bioprotect Res Ctr, Palmerston North 4410, New Zealand. [Ehrlich, Kenneth C.; Cary, Jeffrey W.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Collemare, Jerome; Griffiths, Scott A.; de Wit, Pierre J. G. M.] Wageningen Univ, Phytopathol Lab, NL-6700 AP Wageningen, Netherlands. RP Bradshaw, RE (reprint author), Massey Univ, Inst Mol BioSci, Coll Sci, Sci Tower D,Riddet Rd, Palmerston North 4410, New Zealand. EM r.e.bradshaw@massey.ac.nz RI de Wit, Pierre/A-1998-2013; chettri, pranav/E-4292-2015; Cox, Murray/A-1459-2012; Bradshaw, Rosie/C-7846-2011 OI de Wit, Pierre/0000-0002-4208-288X; Cox, Murray/0000-0003-1936-0236; Bradshaw, Rosie/0000-0001-5228-2685 FU Massey University; New Zealand Bio-Protection Centre of Research Excellence; Royal Society of New Zealand; Graduate School of Experimental Plant Sciences (Netherlands); Willie Commelin Scholten Foundation FX Financial support from Massey University, the New Zealand Bio-Protection Centre of Research Excellence, Royal Society of New Zealand, Graduate School of Experimental Plant Sciences (Netherlands) and Willie Commelin Scholten Foundation is acknowledged. Kimiko Yabe and Hiromitsu Nakajima (National Food Research Institute and Tottori University, Japan) are thanked for advice on the predicted dothistromin biosynthetic pathway. NR 56 TC 13 Z9 14 U1 0 U2 32 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD FEB PY 2013 VL 51 BP 12 EP 20 DI 10.1016/j.fgb.2012.11.006 PG 9 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 086GW UT WOS:000314673100002 PM 23207690 ER PT J AU Colonius, TJ Earley, RW AF Colonius, Tristan J. Earley, Rosemary W. TI One welfare: a call to develop a broader framework of thought and action SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Editorial Material ID ANIMAL-WELFARE; SCIENCE C1 [Colonius, Tristan J.] Amer Assoc Advancement Sci, AAAS Congress Sci & Engn Fellows Program, Washington, DC 20009 USA. USDA, Shelbyville, DE 19975 USA. RP Colonius, TJ (reprint author), Amer Assoc Advancement Sci, AAAS Congress Sci & Engn Fellows Program, 1735 1-2 Swann St NW, Washington, DC 20009 USA. EM tcolonius@gmail.com NR 23 TC 6 Z9 6 U1 5 U2 12 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD FEB 1 PY 2013 VL 242 IS 3 BP 309 EP 310 PG 2 WC Veterinary Sciences SC Veterinary Sciences GA 084SA UT WOS:000314558200004 PM 23327170 ER PT J AU Porporato, PE Filigheddu, N Reano, S Ferrara, M Angelino, E Gnocchi, VF Prodam, F Ronchi, G Fagoonee, S Fornaro, M Chianale, F Baldanzi, G Surico, N Sinigaglia, F Perroteau, I Smith, RG Sun, YX Geuna, S Graziani, A AF Porporato, Paolo E. Filigheddu, Nicoletta Reano, Simone Ferrara, Michele Angelino, Elia Gnocchi, Viola F. Prodam, Flavia Ronchi, Giulia Fagoonee, Sharmila Fornaro, Michele Chianale, Federica Baldanzi, Gianluca Surico, Nicola Sinigaglia, Fabiola Perroteau, Isabelle Smith, Roy G. Sun, Yuxiang Geuna, Stefano Graziani, Andrea TI Acylated and unacylated ghrelin impair skeletal muscle atrophy in mice SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID FOXO TRANSCRIPTION FACTORS; GROWTH-HORMONE RELEASE; P38 MAPK; UBIQUITIN LIGASES; GENE-TRANSCRIPTION; MAMMALIAN TARGET; TRANSGENIC MICE; EXPRESSION; CACHEXIA; METABOLISM AB Cachexia is a wasting syndrome associated with cancer, AIDS, multiple sclerosis, and several other disease states. It is characterized by weight loss, fatigue, loss of appetite, and skeletal muscle atrophy and is associated with poor patient prognosis, making it an important treatment target. Ghrelin is a peptide hormone that stimulates growth hormone (GH) release and positive energy balance through binding to the receptor GHSR-1a. Only acylated ghrelin (AG), but not the unacylated form (UnAG), can bind GHSR-1a; however, UnAG and AG share several GHSR-1a-independent biological activities. Here we investigated whether UnAG and AG could protect against skeletal muscle atrophy in a GHSR-1a-independent manner. We found that both AG and UnAG inhibited dexamethasone-induced skeletal muscle atrophy and atrogene expression through PI3K beta-, mTORC2-, and p38-mediated pathways in myotubes. Upregulation of circulating UnAG in mice impaired skeletal muscle atrophy induced by either fasting or denervation without stimulating muscle hypertrophy and GHSR-1a-mediated activation of the GH/IGF-1 axis. In Ghsr-deficient mice, both AG and UnAG induced phosphorylation of Akt in skeletal muscle and impaired fasting-induced atrophy. These results demonstrate that AG and UnAG act on a common, unidentified receptor to block skeletal muscle atrophy in a GH-independent manner. C1 [Porporato, Paolo E.; Filigheddu, Nicoletta; Reano, Simone; Ferrara, Michele; Angelino, Elia; Gnocchi, Viola F.; Chianale, Federica; Baldanzi, Gianluca; Surico, Nicola; Sinigaglia, Fabiola; Graziani, Andrea] Univ Piemonte Orientale Amedeo Avogadro Alessandr, Dept Translat Med, Interdisciplinary Res Ctr Autoimmune Dis IRCAD, Novara, Vercelli, Italy. [Porporato, Paolo E.; Filigheddu, Nicoletta; Reano, Simone; Ferrara, Michele; Angelino, Elia; Gnocchi, Viola F.; Chianale, Federica; Baldanzi, Gianluca; Surico, Nicola; Sinigaglia, Fabiola; Graziani, Andrea] Univ Piemonte Orientale Amedeo Avogadro Alessandr, Biotechnol Ctr Appl Med Res BRMA, Novara, Vercelli, Italy. [Prodam, Flavia] Univ Piemonte Orientale Amedeo Avogadro Alessandr, Dept Hlth Sci, Novara, Vercelli, Italy. [Ronchi, Giulia; Fornaro, Michele; Graziani, Andrea] Univ Turin, NICO, Orbassano, TO, Italy. [Ronchi, Giulia; Fornaro, Michele; Graziani, Andrea] Univ Turin, Dept Clin & Biol Sci, Orbassano, TO, Italy. [Fagoonee, Sharmila] Univ Turin, Mol Biotechnol Ctr, Turin, Italy. [Fagoonee, Sharmila] Univ Turin, Dept Genet Biol & Biochem, Turin, Italy. [Perroteau, Isabelle] Univ Turin, Dept Life Sci & Syst Biol, Turin, Italy. [Smith, Roy G.] Scripps Res Inst, Dept Metab & Aging, Scripps, FL USA. [Sun, Yuxiang] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Mol & Cellular Biol, Houston, TX 77030 USA. RP Filigheddu, N (reprint author), Univ Piemonte Orientale Amedeo Avogadro, Dept Translat Med, Via Solaroli 17, I-28100 Novara, Italy. EM nicoletta.filigheddu@med.unipmn.it RI Graziani, Andrea/B-2554-2009; Filigheddu, Nicoletta/N-7219-2015; OI Graziani, Andrea/0000-0002-6302-2317; Perroteau, Isabelle/0000-0001-9797-2276; Filigheddu, Nicoletta/0000-0002-3848-611X; Baldanzi, Gianluca/0000-0002-1370-9903; Geuna, Stefano/0000-0002-6962-831X; Fornaro, Michele/0000-0003-1852-9167; Porporato, Paolo Ettore/0000-0001-8519-1552 FU Telethon [GGP030386]; Regione Piemonte CIPE; Regione Piemonte Ricerca Sanitaria; Italian Ministry for University and Research; Opera Pia Eletto Lualdi FX We are grateful to Riccarda Granata and Cristina Grande for insulin measurements and to Thien-Thi Nguyen, Christian Zurlo, Laura Bada, and Giulia Bettas Ardisson for technical assistance. This work was supported by Telethon (grant no. GGP030386 to A. Graziani), Regione Piemonte CIPE (to A. Graziani, S. Geuna, and I. Perroteau), Regione Piemonte Ricerca Sanitaria (to A. Graziani), Italian Ministry for University and Research (PRIN grant to A. Graziani, S. Geuna, and I. Perroteau), and Opera Pia Eletto Lualdi. NR 68 TC 57 Z9 57 U1 0 U2 21 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 2013 VL 123 IS 2 BP 611 EP 622 DI 10.1172/JCI39920 PG 12 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 084QG UT WOS:000314553600018 PM 23281394 ER PT J AU Tilghman, SL Rhodes, LV Bratton, MR Carriere, P Preyan, LC Boue, SM Vasaitis, TS McLachlan, JA Burow, ME AF Tilghman, Syreeta L. Rhodes, Lyndsay V. Bratton, Melyssa R. Carriere, Patrick Preyan, Lynez C. Boue, Stephen M. Vasaitis, Tadas Sean McLachlan, John A. Burow, Matthew E. TI Phytoalexins, miRNAs and Breast Cancer: A Review of Phytochemical-mediated miRNA Regulation in Breast Cancer SO JOURNAL OF HEALTH CARE FOR THE POOR AND UNDERSERVED LA English DT Review DE Phytoalexins; microRNA; breast cancer; estrogen ID RESVERATROL-INDUCED APOPTOSIS; PROSTATE-CANCER; MESENCHYMAL TRANSITION; CAENORHABDITIS-ELEGANS; DOWN-REGULATION; RECEPTOR-ALPHA; HUMAN HEALTH; SMALL RNAS; CELLS; MICRORNAS AB There is growing interest in the diverse signaling pathways that regulate and affect breast tumorigenesis, including the role of phytochemicals and the emerging role of microRNAs (miRNAs). Recent studies demonstrate that miRNAs regulate fundamental cellular and developmental processes at the transcriptional and translational level under normal and disease conditions. While there is growing evidence to support the role of phytoalexin-mediated miRNA regulation of cancer, few reports address this role in breast cancer. Recent reports by our group and others demonstrate that natural products, including stilbenes, curcumin, and glyceollins, could alter the expression of specific miRNAs, which may lead to increased sensitivity of cancer cells to conventional anti-cancer agents and, therefore, hormone-dependent and hormone-independent tumor growth inhibition. This review will discuss how dietary intake of natural products, by regulating specific miRNAs, contribute to the prevention and treatment of breast cancer. C1 [Tilghman, Syreeta L.; Carriere, Patrick; Preyan, Lynez C.] Xavier Univ Louisiana, Coll Pharm, Div Basic Pharmaceut Sci, New Orleans, LA 70125 USA. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Vasaitis, Tadas Sean] Univ Maryland Eastern Shore, Sch Pharm, Dept Pharmaceut Sci, Princess Anne, MD USA. [McLachlan, John A.] Tulane Univ, Ctr Bioenvironm Res, New Orleans, LA 70118 USA. [McLachlan, John A.] Xavier Univ, New Orleans, LA 70125 USA. [Rhodes, Lyndsay V.; Bratton, Melyssa R.; Burow, Matthew E.] Tulane Univ, Sch Med, New Orleans, LA 70112 USA. RP Tilghman, SL (reprint author), Xavier Univ Louisiana, Coll Pharm, Div Basic Pharmaceut Sci, New Orleans, LA 70125 USA. FU NCRR NIH HHS [5G12RR026260, G12 RR026260]; NIMHD NIH HHS [G12 MD007595] NR 59 TC 5 Z9 5 U1 2 U2 28 PU JOHNS HOPKINS UNIV PRESS PI BALTIMORE PA JOURNALS PUBLISHING DIVISION, 2715 NORTH CHARLES ST, BALTIMORE, MD 21218-4363 USA SN 1049-2089 J9 J HEALTH CARE POOR U JI J. Health Care Poor Underserved PD FEB PY 2013 VL 24 IS 1 SU S BP 36 EP 46 PG 11 WC Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA 085LY UT WOS:000314617500006 PM 23395943 ER PT J AU Tsai, JY Villegas-Montoya, C Boland, BB Blasier, Z Egbejimi, O Gonzalez, R Kueht, M McElfresh, TA Brewer, RA Chandler, MP Bray, MS Young, ME AF Tsai, Ju-Yun Villegas-Montoya, Carolina Boland, Brandon B. Blasier, Zachary Egbejimi, Oluwaseun Gonzalez, Raquel Kueht, Michael McElfresh, Tracy A. Brewer, Rachel A. Chandler, Margaret P. Bray, Molly S. Young, Martin E. TI Influence of dark phase restricted high fat feeding on myocardial adaptation in mice SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Article DE Gene expression; High fat feeding; Metabolism; Triglyceride ID INDUCED OBESE MICE; CIRCADIAN CLOCK; CARDIOVASCULAR-DISEASE; CONTRACTILE FUNCTION; DIETARY-FAT; ACID-METABOLISM; GENE-EXPRESSION; HEART; CARDIOMYOCYTE; RISK AB Prolonged high fat feeding is associated with myocardial contractile dysfunction in rodents. However, epidemiological data do not necessarily support the concept that fat-enriched diets adversely affect cardiac function in humans. When fed in an ad libitum manner, laboratory rodents consume chow throughout the day. In contrast, humans typically consume food only during the awake phase. Discrepancies between rodent and human feeding behaviors led us to hypothesize that the time of day at which dietary lipids are consumed significantly influences myocardial adaptation. In order to better mimic feeding behavior in humans, mice were fed (either a control or high fat diet) only during the 12-hour dark phase (i.e., no food was provided during the light phase). We report that compared to dark phase restricted control diet fed mice, mice fed a high fat diet during the dark phase exhibit: 1) essentially normal body weight gain and energy balance; 2) increased fatty acid oxidation at whole body, as well as skeletal and cardiac muscle (in the presence of insulin and/or at high workloads) levels; 3) induction of fatty acid responsive genes, including genes promoting triglyceride turnover in the heart; 4) no evidence of cardiac hypertrophy; and 5) persistence/improvement of myocardial contractile function, as assessed ex vivo. These data are consistent with the hypothesis that ingestion of dietary fat only during the more active/awake period allows adequate metabolic adaptation, thereby preserving myocardial contractile function. This article is part of a Special Issue entitled "Focus on cardiac metabolism". (C) 2012 Elsevier Ltd. All rights reserved. C1 [Tsai, Ju-Yun; Villegas-Montoya, Carolina; Boland, Brandon B.; Blasier, Zachary; Egbejimi, Oluwaseun; Gonzalez, Raquel; Kueht, Michael; Bray, Molly S.; Young, Martin E.] ARS, USDA, Childrens Nutr Res Ctr, Baylor Coll Med,Dept Pediat, Houston, TX USA. [McElfresh, Tracy A.; Chandler, Margaret P.] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA. [Bray, Molly S.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL 35294 USA. [Brewer, Rachel A.; Young, Martin E.] Univ Alabama Birmingham, Dept Med, Div Cardiovasc Dis, Birmingham, AL 35294 USA. RP Young, ME (reprint author), Univ Alabama Birmingham, Dept Med, Div Cardiovasc Dis, 703 19th St S,ZRB 308, Birmingham, AL 35294 USA. EM meyoung@uab.edu FU Kraft Foods Inc.; USDA/ARS [6250-51000-046, 6250-51000-044]; National Heart, Lung, and Blood Institute [HL-074259]; DeBakey Heart Fund at Baylor College of Medicine FX This work was supported by Kraft Foods Inc., the USDA/ARS (6250-51000-046 and 6250-51000-044) and the National Heart, Lung, and Blood Institute (HL-074259). Ju-Yun Tsai was supported by the DeBakey Heart Fund at Baylor College of Medicine. NR 31 TC 15 Z9 16 U1 0 U2 5 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD FEB PY 2013 VL 55 BP 147 EP 155 DI 10.1016/j.yjmcc.2012.09.010 PG 9 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA 085PH UT WOS:000314626200019 PM 23032157 ER PT J AU Kern, CC Montgomery, RA Reich, PB Strong, TF AF Kern, Christel C. Montgomery, Rebecca A. Reich, Peter B. Strong, Terry F. TI Canopy gap size influences niche partitioning of the ground-layer plant community in a northern temperate forest SO JOURNAL OF PLANT ECOLOGY LA English DT Article DE herbaceous layer; experimental gaps; proximity to edge; functional diversity; plant traits ID HEMLOCK-HARDWOOD FORESTS; SPECIES-DIVERSITY; TROPICAL FOREST; HERB LAYER; UNDERSTOREY VEGETATION; LIGHT TRANSMISSION; TREE DIVERSITY; ACER-SACCHARUM; DISTURBANCE; REGENERATION AB The Gap Partitioning Hypothesis (GPH) posits that gaps create heterogeneity in resources crucial for tree regeneration in closed-canopy forests, allowing trees with contrasting strategies to coexist along resource gradients. Few studies have examined gap partitioning of temperate, ground-layer vascular plants. We used a ground-layer plant community of a temperate deciduous forest in northern Wisconsin, USA, as a model system to test whether the GPH extends to the relatively species-rich ground layer. We used a well-replicated experimental approach that included a gap opening gradient (five gap sizes, 6, 10, 20, 30 and 46 m diameter, and undisturbed reference areas), a within-gap location gradient (gap edge to center), and a temporal gradient (0, 2, 6 and 13 years after gap creation). The data were observations of ground-layer plant abundance, published plant traits, and a modeled index of understory light environments. We ordinated the plant abundance data and evaluated the relationships of composition, traits and light environment by gap size, location along the forest-gap transect and time, with several approaches such as correlations, descriptivestatistics, non-parametric tests of group differences and indicator species importance values. Ground-layer plant composition and traits differed across gap sizes, within-gap locations and over time. Gaps of all sizes differed in composition from undisturbed areas, and all pair-wise combinations of gap size also differed in composition, except the 6 m from the 10-m gaps. Large gaps (46 m) also displayed within-gap compositional gradients from gap edge to center locations. Compositional differences in gap size were evident 2 years after gap creation and, contrary to our hypotheses, remained different over the 13-year period, even in gaps with crown closure. In contrast to the neutral theory, species functional traits and microenvironmental conditions were related to variation in ground-layer composition. Species with smaller seeds, lower shade tolerance, later bloom times, shorter stature and longer leaves were associated with higher light, more central gap locations, larger gap sizes and greater time since gap creation. The correlation between gap size and ground-layer plant composition and traits provides evidence for gap partitioning by the diverse ground-layer community in this temperate deciduous forest community. C1 [Kern, Christel C.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Kern, Christel C.; Montgomery, Rebecca A.; Reich, Peter B.] Univ Minnesota Twin Cities, Dept Forest Resources, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Strong, Terry F.] US Forest Serv, USDA, Grand Rapids, MN 55744 USA. RP Kern, CC (reprint author), US Forest Serv, USDA, No Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. EM cckern@fs.fed.us RI Montgomery, Rebecca/J-5774-2013 OI Montgomery, Rebecca/0000-0002-4131-1847 FU US Department of Agriculture Forest Service; Northern Research Station; Chequamegon-Nicolet National Forest; Washington Office Research & Development (Scientist Recruitment Initiative FX US Department of Agriculture Forest Service: specifically, the Northern Research Station (and former North Central Research Station); the Chequamegon-Nicolet National Forest (and former Nicolet National Forest); and the Washington Office Research & Development (Scientist Recruitment Initiative). NR 52 TC 11 Z9 13 U1 12 U2 107 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1752-9921 J9 J PLANT ECOL JI J. Plant Ecol. PD FEB PY 2013 VL 6 IS 1 BP 101 EP 112 DI 10.1093/jpe/rts016 PG 12 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA 086TH UT WOS:000314709400012 ER PT J AU Ahsan, N Huang, YD Tovar-Mendez, A Swatek, KN Zhang, JF Miernyk, JA Xu, D Thelen, JJ AF Ahsan, Nagib Huang, Yadong Tovar-Mendez, Alejandro Swatek, Kirby N. Zhang, Jingfen Miernyk, Jan A. Xu, Dong Thelen, Jay J. TI A Versatile Mass Spectrometry-Based Method to Both Identify Kinase Client-Relationships and Characterize Signaling Network Topology SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE mass spectrometry; kinase; phosphorylation; protein-protein interaction; phosphatase inhibitor; signaling network ID MYOSIN PHOSPHATASE INHIBITOR; PROTEIN-KINASE; PHOSPHORYLATION; SITE; ARABIDOPSIS; IDENTIFICATION; ACTIVATION; EXPRESSION; MECHANISM; REVEALS AB While more than a thousand protein kinases (PK) have been identified in the Arabidopsis thaliana genome, relatively little progress has been made toward identifying their individual client proteins. Herein we describe the use of a mass spectrometry-based in vitro phosphorylation strategy, termed Kinase Client assay (KiC assay), to study a targeted-aspect of signaling. A synthetic peptide library comprising 377 in vivo phosphorylation sequences from developing seed was screened using 71 recombinant A thaliana PK. Among the initial results, we identified 23 proteins as putative clients of 17 PK. In one instance protein phosphatase inhibitor-2 (AtPPI-2) was phosphorylated at multiplesites by three distinct PK, casein kinase1-like 10, ANLE3, and a Ser PK-like protein. To confirm this result, full-length recombinant AtPPI-2 was reconstituted with each of these PK The results confirmed multiple distinct phosphorylation sites within this protein. Biochemical analyses indicate that AtPPI-2 inhibits type 1 protein phosphatase (TOPP) activity, and that the phosphorylated forms of AtPPI-2 are more potent inhibitors. Structural modeling revealed that phosphorylation of AtPPI-2 induces conformational changes that modulate TOPP binding. C1 [Ahsan, Nagib; Huang, Yadong; Tovar-Mendez, Alejandro; Swatek, Kirby N.; Miernyk, Jan A.; Thelen, Jay J.] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. [Ahsan, Nagib; Huang, Yadong; Tovar-Mendez, Alejandro; Swatek, Kirby N.; Zhang, Jingfen; Miernyk, Jan A.; Xu, Dong; Thelen, Jay J.] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO 65211 USA. [Ahsan, Nagib; Huang, Yadong; Tovar-Mendez, Alejandro; Swatek, Kirby N.; Zhang, Jingfen; Xu, Dong; Thelen, Jay J.] Univ Missouri, Christopher S Bond Life Sci Ctr, Columbia, MO 65211 USA. [Zhang, Jingfen; Xu, Dong] Univ Missouri, Dept Comp Sci, Columbia, MO 65211 USA. [Miernyk, Jan A.] Univ Missouri, Plant Genet Res Unit, USDA, ARS, Columbia, MO 65211 USA. RP Thelen, JJ (reprint author), Univ Missouri, Dept Biochem, Christopher S Bond Life Sci Ctr 271G, 1201 Rollins Ave, Columbia, MO 65211 USA. EM thelenj@missouri.edu FU NSF [DBI-0604439] FX The contribution of ML Johnston to the networking graphics presented is gratefully acknowledged. Research in the Thelen Lab is supported by the NSF (Grant No. DBI-0604439). NR 39 TC 8 Z9 8 U1 0 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 J9 J PROTEOME RES JI J. Proteome Res. PD FEB PY 2013 VL 12 IS 2 BP 937 EP 948 DI 10.1021/pr3009995 PG 12 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 084SG UT WOS:000314558800035 PM 23270405 ER PT J AU DellaValle, DM Haas, JD AF DellaValle, Diane M. Haas, Jere D. TI QUANTIFICATION OF TRAINING LOAD AND INTENSITY IN FEMALE COLLEGIATE ROWERS: VALIDATION OF A DAILY ASSESSMENT TOOL SO JOURNAL OF STRENGTH AND CONDITIONING RESEARCH LA English DT Article DE heart rate; perceived exertion; intermittent activities; endurance exercise ID PERCEIVED EXERTION; BLOOD LACTATE; EXERCISE; SCALE; PERFORMANCE; RESPONSES; ACCURACY; SOCCER AB DellaValle, DM and Haas, JD. Quantification of training load and intensity in female collegiate rowers: Validation of a daily assessment tool. J Strength Cond Res 27(2): 540-548, 2013-Session rating of perceived exertion (RPE) has been used to quantify a variety of training activities but has not been tested in rowers, who engage in intermittent patterns of activity. The objective of this study was to evaluate the ability of the subjective session RPE method to quantify on- and off-water rowing training load (TL) compared with a heart rate (HR) summation method. Seven female collegiate rowers (19.7 +/- 0.8 years) were monitored during 2 workout simulations differing in intensity level in the laboratory and several training sessions outside of the laboratory. Training load was calculated using an HR summation method, and RPE was measured after the completion of each simulation or training activity (session RPE). During the workout simulations, despite poor correlation between the HR summation and session RPE methods (r = -0.21, p = 0.52), the session RPE method was significantly correlated with peak WR (r = 0.59, p = 0.05) and posttest blood lactate concentration (r = 0.73, p = 0.007). During training sessions outside of the laboratory, the HR summation (280.7 +/- 108.3) and session RPE (6,432.5 +/- 4,002.0) methods were highly correlated (r = 0.88, p < 0.001). Regression analyses revealed that after controlling for time, training intensity rating was a significant predictor of TL (p, 0.001). We conclude that the session RPE method is a valid metric of TL in female rowers. The method's convenience renders it a feasible option for researchers and coaches to quantify and monitor TL in rowers. C1 [DellaValle, Diane M.] Cornell Univ, USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. RP DellaValle, DM (reprint author), Cornell Univ, USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM dd235@cornell.edu OI DellaValle, Diane/0000-0002-3855-432X FU American Dietetic Association Foundation (Jean Hankin Nutritional Epidemiology Research Grant); Cornell University's Division of Nutritional Sciences FX The authors are grateful to the coaches and rowers of Cornell University's Women's Rowing team that participated in this study. Special thanks go to the undergraduate laboratory assistants (and Cornell University rowers) Kara Achille, Erica Crump, Ray DeVirgiliis, and Sarah Kirsch. This research was funded by grants from the American Dietetic Association Foundation (Jean Hankin Nutritional Epidemiology Research Grant) and Cornell University's Division of Nutritional Sciences. The authors have no conflicts of interest to declare. No funding was received from the NIH, Wellcome Trust, or HHMI. Disclosures: The authors have no conflicts of interest to declare. NR 19 TC 4 Z9 4 U1 1 U2 15 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1064-8011 J9 J STRENGTH COND RES JI J. Strength Cond. Res. PD FEB PY 2013 VL 27 IS 2 BP 540 EP 548 DI 10.1519/JSC.0b013e3182577053 PG 9 WC Sport Sciences SC Sport Sciences GA 086WB UT WOS:000314718600035 PM 22505135 ER PT J AU Manfredi, TG Monteiro, MA Lamont, LS Singh, MF Foldvari, M White, S Cosmas, AC Urso, ML AF Manfredi, Thomas G. Monteiro, Michael A. Lamont, Linda S. Singh, Maria F. Foldvari, Mona White, Sebrina Cosmas, Arthur C. Urso, Maria L. TI POSTMENOPAUSAL EFFECTS OF RESISTANCE TRAINING ON MUSCLE DAMAGE AND MITOCHONDRIA SO JOURNAL OF STRENGTH AND CONDITIONING RESEARCH LA English DT Article DE aging; exercise training; strength; muscle adaptation ID SKELETAL-MUSCLE; OXIDATIVE STRESS; ENZYME-ACTIVITY; HEART-FAILURE; FRAIL ELDERS; OLDER MEN; EXERCISE; WOMEN; STRENGTH; DISEASE AB Manfredi, TG, Monteiro, MA, Lamont, LS, Singh, MF, Foldvari, M, White, S, Cosmas, AC, and Urso, ML. Postmenopausal effects of resistance training on muscle damage and mitochondria. J Strength Cond Res 27(2): 556-561, 2013-The purpose of this study was to measure the effects of a 12-month progressive resistance training intervention on muscle morphology and strength gains in postmenopausal women. Skeletal muscle biopsies were obtained from the vastus lateralis of 5 independent community-dwelling women (mean age: 75.6 +/- 4.28 years; mean height: 163 +/- 5.34 cm; mean weight: 72 +/- 17.5 kg) before 6 months and 12 months after progressive resistance training. Muscle strength (1 repetition maximum) was measured at the same time points. After 6 months of training, morphological analysis revealed evidence of increased proteolysis and tissue repair, and rudimentary fiber development. The percent of Z-bands with mild Z-band disruption increased from 43.9% at baseline to 66.7% after 6 months of training (p < 0.01). Mitochondrial volume also increased (percent of mitochondria = 0.86% at baseline, 1.19% at 6 months, and 1.04% at 12 months, p < 0.05), and there was a shift to larger sized mitochondria. The training did not result in statistically significant increases in muscle leg strength (p < 0.18). It appears that mild Z-band disruption acts as a precursor for increased protein synthesis and stimulates an increase in mitochondrial mass. Therefore, although a progressive resistance training program in this population did not increase muscle strength, it did demonstrate clinical applications that lend support to the importance of resistance training in older adults. C1 [Manfredi, Thomas G.; Monteiro, Michael A.; Lamont, Linda S.; White, Sebrina; Cosmas, Arthur C.] Univ Rhode Isl, Energy Metab Lab, Kingston, RI 02881 USA. [Manfredi, Thomas G.; Monteiro, Michael A.; Lamont, Linda S.; White, Sebrina; Cosmas, Arthur C.] Univ Rhode Isl, Dept Kinesiol, Kingston, RI 02881 USA. [Singh, Maria F.; Foldvari, Mona] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Singh, Maria F.] Univ Sydney, Sch Exercise & Sport Sci, Lidcombe, NSW, Australia. [Cosmas, Arthur C.] Select Med Sports Med & Outpatient Rehabil, Hartford, CT USA. [Urso, Maria L.] USA, Mil Performance Div, Inst Environm Med USARIEM, Natick, MA 01760 USA. RP Urso, ML (reprint author), USA, Mil Performance Div, Inst Environm Med USARIEM, Natick, MA 01760 USA. EM maria.urso@us.army.mil FU National Institutes of Health, Institute of General Medical Sciences, Bridges to the Future [R25 GM51780-02]; University of Rhode Island FX M. Monteiro was supported by a grant from the National Institutes of Health, Institute of General Medical Sciences, Bridges to the Future Grant R25 GM51780-02 awarded to the Kinesiology department (L. S. Lamont), University of Rhode Island. Disclaimer: The opinions or assertions contained herein are the private views of the author(s) and are not to be construed as official or as reflecting the views of the Army or the Department of Defense. NR 29 TC 1 Z9 1 U1 0 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1064-8011 J9 J STRENGTH COND RES JI J. Strength Cond. Res. PD FEB PY 2013 VL 27 IS 2 BP 556 EP 561 PG 6 WC Sport Sciences SC Sport Sciences GA 086WB UT WOS:000314718600037 PM 23090317 ER PT J AU Rasaputra, KS Liyanage, R Lay, JO Slavik, MF Rath, NC AF Rasaputra, Komal Singh Liyanage, Rohana Lay, Jackson O., Jr. Slavik, Michael F. Rath, Narayan C. TI Effect of thiram on avian growth plate chondrocytes in culture SO JOURNAL OF TOXICOLOGICAL SCIENCES LA English DT Article DE Thiram; Chondrocyte; Proteins; Chicken; Cell death ID HEAT-SHOCK PROTEINS; TIBIAL DYSCHONDROPLASIA; ALDEHYDE DEHYDROGENASE; TETRAMETHYLTHIURAM DISULFIDE; MOLECULAR CHAPERONE; PESTICIDE EXPOSURE; RISK-ASSESSMENT; CELL-DEATH; CARTILAGE; INDUCTION AB Thiram is a dithiocarbamate pesticide that causes tibial dyschondroplasia (TD), a growth plate defect, in poultry. Deaths of transitional zone chondrocytes appear to interrupt endochondral bone development leading to the broadening of growth plate. The mechanism of action of thiram on chondrocytes is not well understood. Since proteins play major roles in different aspects of cell's metabolism, growth, and survival, the objective of this study was to find whether thiram produces proteomic changes that could impair the development of chondrocytes. The chondrocytes, isolated from proximal tibial growth plates, were cultured with or without a sub-lethal concentration of thiram for 48 hr, and the cell proteins were extracted, and subjected to 2-D gel electrophoresis. The gel images were compared and statistically evaluated using Melanie software to identify differentially expressed protein spots. Of a total of 72 identifiable spots 3 were down-regulated and 2 up-regulated in thiram treated chondrocytes. In-gel trypsin digestion of the protein spots followed by their characterization by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry identified 25 spots comprising of 23 proteins. Two of 3 down-regulated proteins were identified as a heat shock protein 70 (HSP 70) and a GALE (UDP-galactose-4 epimerase) protein isoform I. The up-regulated proteins were Serpin H1, a protein involved in collagen metabolism and a redox sensor NmrA-like (NMRAL) family domain protein-1. Both GALE and NMRAL proteins are implicated in energy metabolism and redox regulation whereas the HSP 70 protects cells against stress, and implicated in chondrocyte hypertrophy, an important event in endochondral bone formation. The failure of chondrocyte protective mechanisms such as associated with protection against cellular stress and energy metabolism appear to be the likely cause for chondrocyte death induced by thiram. C1 [Rasaputra, Komal Singh; Rath, Narayan C.] Univ Arkansas, USDA ARS, Poultry Sci Ctr, Fayetteville, AR 72701 USA. [Rasaputra, Komal Singh; Slavik, Michael F.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Liyanage, Rohana; Lay, Jackson O., Jr.] Univ Arkansas, State Wide Mass Spectrometry Facil, Fayetteville, AR 72701 USA. RP Rath, NC (reprint author), Univ Arkansas, USDA ARS, Poultry Sci Ctr, Fayetteville, AR 72701 USA. EM narayan.rath@ars.usda.gov RI Lay, Jackson/G-1007-2011 OI Lay, Jackson/0000-0003-3789-2527 NR 66 TC 1 Z9 4 U1 1 U2 7 PU JAPANESE SOC TOXICOLOGICAL SCIENCES PI TOKYO PA INTERNATIONAL MEDICAL INFORMATION CENTER, SHINANOMACHI RENGAKAN, 35 SHINANO-MACHI, SHINJUKU-KU, TOKYO, 160-0016, JAPAN SN 0388-1350 EI 1880-3989 J9 J TOXICOL SCI JI J. Toxicol. Sci. PD FEB PY 2013 VL 38 IS 1 BP 93 EP 101 PG 9 WC Toxicology SC Toxicology GA 086GE UT WOS:000314671300010 PM 23358143 ER PT J AU Poessel, SA Breck, SW Teel, TL Shwiff, S Crooks, KR Angeloni, L AF Poessel, Sharon A. Breck, Stewart W. Teel, Tara L. Shwiff, Stephanie Crooks, Kevin R. Angeloni, Lisa TI Patterns of human-coyote conflicts in the Denver Metropolitan Area SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Canis latrans; conflict; coyote; Denver; sighting; spatial; urban ID HABITAT FRAGMENTATION; URBAN-ENVIRONMENT; AVAILABILITY DATA; HOME-RANGE; URBANIZATION; LANDSCAPE; ECOLOGY; USA; CONSERVATION; BIODIVERSITY AB In many cities throughout North America, humancoyote conflicts are an emerging problem. Little research has described temporal and spatial patterns of humancoyote conflicts, although such information can be an important step in developing and optimizing management efforts. We used reports from 22 entities within the Denver Metropolitan Area (DMA) in Colorado that provided information on coyote observations (signs, sightings, and encounters) and conflicts (incidents, pet-attacks, and human-attacks). Our objectives were to 1) define, quantify, and map categories of coyote observations and conflicts, and 2) investigate temporal and spatial patterns of conflicts, particularly related to land cover types and housing density classes. We compiled 4,006 coyote reports, including 78 signs (1.9%), 3,023 sightings (75.5%), 395 encounters (9.9%), 26 incidents (0.6%), 471 pet-attacks (11.8%), and 13 human-attacks (0.3%). We found a strong seasonal pattern with reports of both observations and conflicts highest during DecemberMarch and lowest during JulySeptember. Numbers of coyote conflicts were disproportionately greater in open space and development land cover types (in contrast to natural and agricultural land cover) and in suburban housing areas (in contrast to urban, exurban, and rural areas). Hotspots of coyote conflicts were apparent in the southern region of the DMA, possibly because coyotes had better access to development, and hence interaction with residents, via natural areas bordering urban areas; reporting bias may have also influenced this outcome. Our results will help target management efforts, particularly those focused on people (e.g., education), but also highlight the critical need for improved methods of collecting conflict information via a standardized reporting mechanism to help reduce bias. (C) 2012 The Wildlife Society. C1 [Poessel, Sharon A.; Crooks, Kevin R.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Breck, Stewart W.; Shwiff, Stephanie] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Teel, Tara L.] Colorado State Univ, Dept Human Dimens Nat Resources, Ft Collins, CO 80523 USA. [Angeloni, Lisa] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. RP Poessel, SA (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM stewart.w.breck@aphis.usda.gov RI Poessel, Sharon/B-3651-2013 OI Poessel, Sharon/0000-0002-0283-627X FU Adams County; City of Lakewood; City of Aurora; City and County of Denver; Jefferson County; Colorado State University; USDA-WS-National Wildlife Research Center FX The following entities provided funding to support this research: Adams County, City of Lakewood, City of Aurora, City and County of Denver, Jefferson County, Colorado State University, and USDA-WS-National Wildlife Research Center. We thank the employees of Colorado Parks and Wildlife, USDA-Wildlife Services-Colorado, and each of the additional 21 entities in the DMA that provided their data on coyote observations and conflicts. We thank the City of Aurora for providing their initial map of coyote reports containing the categories we eventually used for this study. We appreciate the assistance of C. Burdett in obtaining spatial datasets. We also thank J. Daigle and 2 anonymous referees for providing comments that improved the manuscript. NR 45 TC 15 Z9 15 U1 17 U2 155 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2013 VL 77 IS 2 BP 297 EP 305 DI 10.1002/jwmg.454 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 079NH UT WOS:000314176900010 ER PT J AU Chaparro, JM Badri, DV Bakker, MG Sugiyama, A Manter, DK Vivanco, JM AF Chaparro, Jacqueline M. Badri, Dayakar V. Bakker, Matthew G. Sugiyama, Akifumi Manter, Daniel K. Vivanco, Jorge M. TI Root Exudation of Phytochemicals in Arabidopsis Follows Specific Patterns That Are Developmentally Programmed and Correlate with Soil Microbial Functions SO PLOS ONE LA English DT Article ID AGROBACTERIUM-TUMEFACIENS; GENE-EXPRESSION; PSEUDOMONAS-FLUORESCENS; SINORHIZOBIUM-MELILOTI; RHIZOSPHERE MICROBIOME; SECONDARY METABOLITES; BACILLUS-SUBTILIS; CHEMOTAXIS; ACID; SECRETION AB Plant roots constantly secrete compounds into the soil to interact with neighboring organisms presumably to gain certain functional advantages at different stages of development. Accordingly, it has been hypothesized that the phytochemical composition present in the root exudates changes over the course of the lifespan of a plant. Here, root exudates of in vitro grown Arabidopsis plants were collected at different developmental stages and analyzed using GC-MS. Principle component analysis revealed that the composition of root exudates varied at each developmental stage. Cumulative secretion levels of sugars and sugar alcohols were higher in early time points and decreased through development. In contrast, the cumulative secretion levels of amino acids and phenolics increased over time. The expression in roots of genes involved in biosynthesis and transportation of compounds represented in the root exudates were consistent with patterns of root exudation. Correlation analyses were performed of the in vitro root exudation patterns with the functional capacity of the rhizosphere microbiome to metabolize these compounds at different developmental stages of Arabidopsis grown in natural soils. Pyrosequencing of rhizosphere mRNA revealed strong correlations (p<0.05) between microbial functional genes involved in the metabolism of carbohydrates, amino acids and secondary metabolites with the corresponding compounds released by the roots at particular stages of plant development. In summary, our results suggest that the root exudation process of phytochemicals follows a developmental pattern that is genetically programmed. C1 [Chaparro, Jacqueline M.; Badri, Dayakar V.; Bakker, Matthew G.; Vivanco, Jorge M.] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. [Sugiyama, Akifumi] Kyoto Univ, Res Inst Sustainable Humanosphere, Uji, Kyoto, Japan. [Manter, Daniel K.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80522 USA. RP Vivanco, JM (reprint author), Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. EM j.vivanco@colostate.edu RI Bakker, Matthew/B-8610-2013 OI Bakker, Matthew/0000-0003-0345-0587 FU National Science Foundation [MCB-0950857] FX Work was supported by a grant from the National Science Foundation to J.M.V. (MCB-0950857). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 68 TC 92 Z9 95 U1 14 U2 231 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 1 PY 2013 VL 8 IS 2 AR e55731 DI 10.1371/journal.pone.0055731 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 085EU UT WOS:000314597900053 PM 23383346 ER PT J AU Silva, MSE Ellis, A Karaca, K Minke, J Nordgren, R Wu, SX Swayne, DE AF Sa e Silva, Mariana Ellis, Angela Karaca, Kemal Minke, Jules Nordgren, Robert Wu, Shixuan Swayne, David E. TI Domestic goose as a model for West Nile virus vaccine efficacy SO VACCINE LA English DT Article DE Efficacy; Goose; Vaccine; West Nile virus ID GEESE ANSER-ANSER; NEUTRALIZING ANTIBODIES; TRANSMISSION; ENCEPHALITIS; CHALLENGE; INFECTION; OUTBREAK; DISEASE; FEVER; BIRDS AB West Nile virus (WNV) is an emergent pathogen in the Americas, first reported in New York during 1999, and has since spread across the USA, Central and South America causing neurological disease in humans, horses and some bird species, including domestic geese. No WNV vaccines are licensed in the USA for use in geese. This study reports the development of a domestic goose vaccine efficacy model, based on utilizing multiple parameters to determine protection. To test the model, 47 geese were divided in two experiments, testing five different vaccine groups and two sham groups (challenged and unchallenged). Based on the broad range of results for individual metrics between the Challenged-Sham and Unchallenged-Sham groups, the best parameters to measure protection were Clinical Pathogenicity Index (CPI), plasma virus positive geese on days 1-4 post-inoculation and plasma virus titers, and brain histological lesion rates and severity scores. Compared to the Challenged-Sham group, the fowlpox virus vectored vaccine with inserts of WNV prM and E proteins (vFP2000) provided the best protection with significant differences in all five metrics, followed by the canarypox virus vectored vaccine with inserts of WNV prM and E proteins (vCP2018) with four metrics of protection, recombinant vCP2017 with three metrics and WNV E protein with one. These data indicate that domestic geese can be used in an efficacy model for vaccine protection studies using clinical, plasma virological and brain histopathological parameters to evaluate protection against WNV challenge. Published by Elsevier Ltd. C1 [Sa e Silva, Mariana; Swayne, David E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Ellis, Angela] Univ Georgia, Vet Med Diagnost Lab, Coll Vet Med, Athens, GA 30606 USA. [Karaca, Kemal] Pfizer Anim Hlth, Kalamazoo, MI 49007 USA. [Minke, Jules; Nordgren, Robert] Merial Ltd, Duluth, GA 30096 USA. [Wu, Shixuan] ARS, USDA, Manhattan, KS 66502 USA. RP Swayne, DE (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM David.Swayne@ars.usda.gov NR 23 TC 4 Z9 4 U1 0 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD FEB PY 2013 VL 31 IS 7 BP 1045 EP 1050 DI 10.1016/j.vaccine.2012.12.044 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 083TA UT WOS:000314486900007 ER PT J AU Ranger, CM Reding, ME Schultz, PB Oliver, JB AF Ranger, Christopher M. Reding, Michael E. Schultz, Peter B. Oliver, Jason B. TI Influence of flood-stress on ambrosia beetle host-selection and implications for their management in a changing climate SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Ambrosia beetles; ethanol; flood-stress; Scolytinae; SPME-GC-MS ID ETHANOL-BAITED TRAPS; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; DOUGLAS-FIR; XYLOSANDRUS-GERMANUS; POPULUS-DELTOIDES; TREES; ACETALDEHYDE; ATTRACTION; VOLATILES; EXTREMES AB 1 Xylosandrus germanus (Blandford) is a key pest of ornamental nursery trees. Ethanol is the most attractive semiochemical known for X. germanus, and its emission from trees represents a primary host-selection cue. Ethanol production is induced by a variety of abiotic and biotic stressors, which could thereby predispose trees to attack by ethanol-responsive ambrosia beetles. 2 To better understand X. germanus host-selection behaviour within ornamental nurseries, a series of experiments examined the influence of flood-stress on the attractiveness and susceptibility of flowering dogwood Cornus florida L. Under field conditions, more X. germanus were attracted to experimentally flood-stressed dogwoods than neighbouring nonflooded controls in 2009, 2010 and 2011. Flood-stressed dogwoods were also preferentially attacked in 2009-2011, although no attacks occurred on any of the neighbouring nonflooded trees. 3 Solid-phase microextraction-gas chromatography-mass spectrometry detected ethanol in stem tissue from flooded dogwoods but not nonflooded trees. Acetaldehyde, acetic acid and ethanol were also emitted from the outer bark of flooded dogwoods but not nonflooded trees. 4 These results demonstrate that X. germanus preferentially lands on and attacks physiologically-stressed hosts, and further support the role of ethanol in mediating this interaction. 5 Attacks by X. germanus have previously been suspected to occur on trees viewed as apparently-healthy, although the possibility of such trees being in apparently-stressed at the time of attack cannot be ruled out given the results obtained in the present study. Minimizing the impact of stressors known to induce the production of ethanol should be the primary foundation of a management plan for X. germanus and other ethanol-responsive ambrosia beetles. C1 [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Applicat Technol Res Unit,Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Schultz, Peter B.] Virginia Tech, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA 23455 USA. [Oliver, Jason B.] Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, Mcminnville, TN 37110 USA. RP Ranger, CM (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Applicat Technol Res Unit,Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU USDA Floriculture and Nursery Research Initiative; ARS [3607-22000-012-00D] FX This research was supported in part by funding from the USDA Floriculture and Nursery Research Initiative and base funds associated with ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine). We thank James Moyseenko, Gerald Hammel, Jennifer Barnett, Betsy Anderson, Leslie Morris and Leona Horst for technical assistance, as well as Mark Seiderman (NOAA-National Climatic Data Center) for assistance with meteorological data. We also thank the anonymous reviewers for useful comments. The mention of proprietary products or companies does not imply any endorsement or preferential treatment by the USDA-Agricultural Research Service, The Ohio State University, Virginia Tech or Tennessee State University. NR 48 TC 15 Z9 16 U1 1 U2 58 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2013 VL 15 IS 1 BP 56 EP 64 DI 10.1111/j.1461-9563.2012.00591.x PG 9 WC Entomology SC Entomology GA 075KV UT WOS:000313885500006 ER PT J AU Geyer, WA Ponder, F AF Geyer, Wayne A. Ponder, Felix, Jr. TI Transferring site information for black walnut from native woodlands in southeastern Kansas USA to identify sites for agroforestry practices SO AGROFORESTRY SYSTEMS LA English DT Article DE Juglans nigra; Southeast Kansas; Site; Site index; Soil; Restrictive soil depth ID GROWTH AB Black walnut (Juglans nigra) is an important tree species for temperate agroforestry in the United States for timber, nuts, wildlife, and abrasives. Predictions of forestland productivity are needed for proper species selection in tree planting. Potential productivity can be estimated for non-forested areas and agricultural croplands by relating site quality to site and soil characteristics. Our study measured the growth potential of black walnut in natural stands. We looked at over 210 stands on unglaciated soils in southeastern Kansas with the site index at 50 years ranging from 9.1 to 26.8 m. Numerous environmental factors were evaluated, including: site, soil chemical, and soil physical properties. These environmental factors were related to tree height at the standard age of 50 years. Simple correlation and multiple regression analyses were run with the site index using over 60 variables of soil and topographic characteristics. Understory competition had no effect on site index. Chemical characteristics had a slight effect on site index. We explained 73 % of the variation in site index with just two variables; depth to a restrictive layer (DRL) and soil type. DRL is a good predictor of walnut productivity. C1 [Geyer, Wayne A.] Kansas State Univ, Div Forestry, Manhattan, KS 66506 USA. [Ponder, Felix, Jr.] US Forest Serv, No Res Stn, USDA, Columbia, MO 65211 USA. RP Geyer, WA (reprint author), Kansas State Univ, Div Forestry, Throckmorton Hall, Manhattan, KS 66506 USA. EM wgeyer@ksu.edu NR 27 TC 1 Z9 1 U1 2 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 J9 AGROFOREST SYST JI Agrofor. Syst. PD FEB PY 2013 VL 87 IS 1 BP 13 EP 18 DI 10.1007/s10457-012-9518-4 PG 6 WC Agronomy; Forestry SC Agriculture; Forestry GA 080UU UT WOS:000314270400002 ER PT J AU Sen, S Iyer, C Klebenov, D Histed, A Aviles, JA Meydani, SN AF Sen, Sarbattama Iyer, Chitra Klebenov, David Histed, Alexander Aviles, Jessica A. Meydani, Simin N. TI Obesity impairs cell-mediated immunity during the second trimester of pregnancy SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article; Proceedings Paper CT Joint Annual Meeting of the Pediatric-Academic-Societies and Asian-Society-for-Pediatric-Research CY APR 30-MAY 03, 2011 CL Denver, CO SP Pediat Acad Soc, Asian Soc Pediat Res DE immune compromise; maternal obesity; perinatal infection ID TUMOR-NECROSIS-FACTOR; MATERNAL OBESITY; HUMAN DECIDUA; T-CELLS; INFLAMMATION; ENVIRONMENT; OVERWEIGHT; OUTCOMES; STRESS; IMPACT AB OBJECTIVE: Obese pregnancy is associated with significantly higher rates of infection, which can harm both mother and fetus. The objective of this study was to determine the impact of obesity on maternal blood immune function. STUDY DESIGN: This was a cross-sectional, case control study of 15 obese (Ob) and 15 lean (Lc) subjects. Immune cell subsets, intracellular and serum cytokine production, and lymphocyte proliferation were measured in maternal blood during the second trimester of pregnancy. RESULTS: Obese women had a significantly lower proportion of CD8+ and NKT cells and a higher proportion of B cells, impaired cytokine production when stimulated ex vivo, and impaired ability of lymphocytes to proliferate compared with their lean counterparts. CONCLUSION: Obese pregnancy is associated with impaired cell-mediated immunity. Because perinatal infections can have serious maternal and fetal consequences, it is imperative to better understand these mechanistic underpinnings to optimize prevention and devise targeted therapy. C1 [Sen, Sarbattama; Histed, Alexander; Meydani, Simin N.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Sen, Sarbattama] Tufts Med Ctr, Mother Infant Res Inst, Boston, MA 02111 USA. [Iyer, Chitra] Tufts Med Ctr, Dept Obstet & Gynecol, Div Newborn Med, Boston, MA 02111 USA. [Sen, Sarbattama; Histed, Alexander] Tufts Med Ctr, Dept Pediat, Boston, MA 02111 USA. [Aviles, Jessica A.] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. RP Sen, S (reprint author), Tufts Med Ctr, Mother Infant Res Inst, 800 Washington St,TMC Box 44, Boston, MA 02111 USA. EM ssen@tuftsmedicalcenter.org NR 38 TC 1 Z9 1 U1 0 U2 7 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD FEB PY 2013 VL 208 IS 2 AR 139.e1 DI 10.1016/j.ajog.2012.11.004 PG 8 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 077HO UT WOS:000314018700019 PM 23159744 ER PT J AU Crosslin, JM AF Crosslin, James M. TI Introduction to 2009 International Symposium on Potato Virus Y SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Editorial Material DE Potato diseases; Plant viruses; PVY AB Potato virus Y (PVY) is one of the most, if not the most, economically important viruses of potato. This virus occurs worldwide wherever the crop is grown. Infections with PVY reduce both yield and quality of the potato crop. The virus occurs as numerous biological strains that differ in pathogenicity on potato and tobacco and vary considerably in their serological and molecular genetic characteristics. The purpose of this symposium is to provide up-to-date information on the characteristics of this important virus, details of its genetic make-up and variability, research efforts in North America, and Europe, and an outline of the workings of a potato virus seed certification effort. C1 USDA ARS, Prosser, WA 99350 USA. RP Crosslin, JM (reprint author), USDA ARS, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM jim.crosslin@ars.usda.gov NR 0 TC 1 Z9 1 U1 0 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2013 VL 90 IS 1 BP 1 EP 1 DI 10.1007/s12230-012-9283-y PG 1 WC Agronomy SC Agriculture GA 080WU UT WOS:000314275600001 ER PT J AU Crosslin, JM AF Crosslin, James M. TI PVY: An Old Enemy and A Continuing Challenge SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato diseases; Plant viruses; PVY ID POTATO-VIRUS-Y; MULTIPLEX RT-PCR; MONOCLONAL-ANTIBODIES; RUSSET NORKOTAH; UNITED-STATES; 3-PRIMER PCR; STRAINS; TRANSMISSION; APHID; DIFFERENTIATION AB Potato virus Y (PVY) is one of the most important viruses affecting potato production worldwide. The virus has been extensively studied for several decades, yet considerable economic losses continue to be suffered by the potato industry around the world. PVY is transmitted by several species of aphids in a nonpersistent manner, making control with insecticides difficult. Additionally, the virus occurs as several distinct strains, each with their own biological, serological, and molecular characteristics. This wide diversity continues to be a challenge to researchers, growers, and processors wherever the crop is grown. Here I will examine the general characteristics of PVY and touch upon some of the major facets of this important and variable virus. C1 USDA ARS, Prosser, WA 99350 USA. RP Crosslin, JM (reprint author), USDA ARS, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM jim.crosslin@ars.usda.gov FU Plant Protection Section of the Potato Association of America in Fredericton, New Brunswick, Canada FX This paper is an invited presentation as part of the International Symposium on PVY sponsored by the Plant Protection Section of the Potato Association of America, August 11, 2009, in Fredericton, New Brunswick, Canada. This paper was reviewed with a view to ensure that the information was brought up-to-date beyond what was given at the time of the presentation. NR 39 TC 4 Z9 6 U1 1 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2013 VL 90 IS 1 BP 2 EP 6 DI 10.1007/s12230-012-9286-8 PG 5 WC Agronomy SC Agriculture GA 080WU UT WOS:000314275600002 ER PT J AU Karasev, AV Gray, SM AF Karasev, Alexander V. Gray, Stewart M. TI Genetic Diversity of Potato virus Y Complex SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE PVY; Strains; Classification ID TUBER NECROTIC RINGSPOT; MULTIPLEX PCR ASSAY; NUCLEOTIDE-SEQUENCE; UNITED-STATES; WHOLE GENOME; RT-PCR; SIMULTANEOUS DIFFERENTIATION; SEROLOGICAL PROPERTIES; STRAIN GROUPS; PVYN-O AB Potato virus Y (PVY) has emerged as a significant problem in all potato-producing areas, including North America. PVY exists as a complex of strains producing a range of disease symptoms in various potato cultivars leading to yield reduction, and some of these strains are known to affect tuber quality. In the past 30 years, significant changes in PVY strains circulating in potato crops have been observed in Europe, and more recently in North America, with an increased incidence of PVY strains associated with potato tuber damage. Different models have been proposed to explain these changes, including spread of new recombinants, enhanced vector transmission of certain strains, or introduction of new potato varieties. Here, we analyze the current knowledge of PVY genetic diversity with an emphasis on PVY strains common in North America. Multiple types of PVY genome recombinants with links to specific symptoms in potato varieties are described and discussed. Different approaches to distinguish PVY strains are reviewed and compared, including biological and laboratory methods. C1 [Karasev, Alexander V.] Univ Idaho, Dept PSES, Moscow, ID 83844 USA. [Gray, Stewart M.] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. RP Karasev, AV (reprint author), Univ Idaho, Dept PSES, Moscow, ID 83844 USA. EM akarasev@uidaho.edu FU Plant Protection Section of the Potato Association of America in Fredericton, New Brunswick, Canada; USDA-NIFA-NRI [2009-35600-05025]; USDA-NIFA-SCRI [2009-51181-05894]; U.S. Potato Board; USDA-ARS [58-5354-7-540, 58-1907-8-870]; Idaho Potato Commission FX This paper is an invited presentation as part of the International Symposium on PVY sponsored by the Plant Protection Section of the Potato Association of America, August 11, 2009, in Fredericton, New Brunswick, Canada. This paper was reviewed with a view to ensure that the information was brought up-to-date beyond what was given at the time of the presentation.; The authors would like to thank James Lorenzen and Camille Kerlan for many helpful discussions. This work was funded in part through grants from USDA-NIFA-NRI (#2009-35600-05025), USDA-NIFA-SCRI (#2009-51181-05894), U.S. Potato Board, the USDA-ARS Cooperative Agreements 58-5354-7-540 and 58-1907-8-870, and the Idaho Potato Commission. NR 55 TC 13 Z9 15 U1 3 U2 39 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2013 VL 90 IS 1 BP 7 EP 13 DI 10.1007/s12230-012-9287-7 PG 7 WC Agronomy SC Agriculture GA 080WU UT WOS:000314275600003 ER PT J AU Rondon, SI Brown, CR Marchosky, R AF Rondon, Silvia I. Brown, Charles R. Marchosky, Ruben TI Screening for Resistance of Potato Lines to the Potato Tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Clones; Columbia basin; Germplasm; Insects; Phthorimaea operculella; Potato; Potato tubermoth; Resistance ID BACILLUS-THURINGIENSIS; CULTURAL-PRACTICES; COLUMBIA BASIN; MOTH; DAMAGE; MANAGEMENT; GERMPLASM; BIOLOGY; ECOLOGY; SOLANUM AB The potato tuberworm, Phthorimaea operculella (Zeller) (PTW), is one of the most important limitations to potato, Solanum tuberosum L., productivity worldwide. Leaves, stems, petioles and more importantly, potato tubers, in the field and storage can be seriously affected. Due to the relatively recent arrival of the PTW in the United States Pacific Northwest, the local and regional lines have never been screened for tuber resistance to PTW. Thus, the objective of this study was to screen regional potato lines for potential PTW resistance by tubers under field and laboratory conditions. Experiments were conducted at the Hermiston Agricultural Research and Extension Center in Hermiston Oregon in 2006 and 2007. Accessions that had few number of mines per tuber were AC97521-1R/Y, Q174-2, Rubi, Yuguima, Paciencia, TM-3, KWPTM24 and CIP 780660; lines than had few larvae per tuber were A97287-6, PA00N10-5, AC97521-1R/Y, Q174-2, PA04LNC2-1, PA04LNC4-1, TM-3 and CIP 780660. Incorporating host plant resistance to tuber penetration by PTW larvae together with appropriate cultural practices including limitation of exposure time of tubers in the field and judicious use of chemicals may provide the best sustainable management option. C1 [Rondon, Silvia I.; Marchosky, Ruben] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [Brown, Charles R.] USDA ARS, Prosser, WA 99350 USA. RP Rondon, SI (reprint author), Oregon State Univ, Hermiston Agr Res & Extens Ctr, 2121 S 1st St, Hermiston, OR 97838 USA. EM silvia.rondon@oregonstate.edu FU Oregon Potato Commission; Western IPM center FX We thank the Pacific Northwest Tri-State Potato Program, Salomon Yilma and Drs. David Douches, Walter DeJong, Dan Hane, Isabel Vales and Rich Novy for providing plant material. The authors also thank Chiho Kimoto, Mahmut Dogramaci, and Laurie Leroux for collecting and organizing the data. We gratefully acknowledge the Oregon Potato Commission and Western IPM center for financial support. NR 30 TC 1 Z9 1 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2013 VL 90 IS 1 BP 71 EP 82 DI 10.1007/s12230-012-9278-8 PG 12 WC Agronomy SC Agriculture GA 080WU UT WOS:000314275600012 ER PT J AU Donovan, GH Butry, DT Michael, YL Prestemon, JP Liebhold, AM Gatziolis, D Mao, MY AF Donovan, Geoffrey H. Butry, David T. Michael, Yvonne L. Prestemon, Jeffrey P. Liebhold, Andrew M. Gatziolis, Demetrios Mao, Megan Y. TI The Relationship Between Trees and Human Health Evidence from the Spread of the Emerald Ash Borer SO AMERICAN JOURNAL OF PREVENTIVE MEDICINE LA English DT Article ID PHYSICAL-ACTIVITY; STREET TREES; GREEN SPACE; ASTHMA; INEQUALITIES; MORTALITY; EXPOSURE; DISEASE; FOREST; AREAS AB Background: Several recent studies have identified a relationship between the natural environment and improved health outcomes. However, for practical reasons, most have been observational, cross-sectional studies. Purpose: A natural experiment, which provides stronger evidence of causality, was used to test whether a major change to the natural environment-the loss of 100 million trees to the emerald ash borer, an invasive forest pest-has influenced mortality related to cardiovascular and lower-respiratory diseases. Methods: Two fixed-effects regression models were used to estimate the relationship between emerald ash borer presence and county-level mortality from 1990 to 2007 in 15 U. S. states, while controlling for a wide range of demographic covariates. Data were collected from 1990 to 2007, and the analyses were conducted in 2011 and 2012. Results: There was an increase in mortality related to cardiovascular and lower-respiratory-tract illness in counties infested with the emerald ash borer. The magnitude of this effect was greater as infestation progressed and in counties with above-average median household income. Across the 15 states in the study area, the borer was associated with an additional 6113 deaths related to illness of the lower respiratory system, and 15,080 cardiovascular-related deaths. Conclusions: Results suggest that loss of trees to the emerald ash borer increased mortality related to cardiovascular and lower-respiratory-tract illness. This finding adds to the growing evidence that the natural environment provides major public health benefits. (Am J Prev Med 2013; 44(2): 139-145) Published by Elsevier Inc. on behalf of American Journal of Preventive Medicine C1 [Donovan, Geoffrey H.; Gatziolis, Demetrios; Mao, Megan Y.] US Forest Serv, USDA, PNW Res Stn, Portland, OR 97205 USA. [Butry, David T.] NIST, Gaithersburg, MD 20899 USA. [Michael, Yvonne L.] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19104 USA. [Prestemon, Jeffrey P.] US Forest Serv, USDA, So Res Stn, Res Triangle Pk, NC USA. [Liebhold, Andrew M.] US Forest Serv, No Res Stn, USDA, Morgantown, WV USA. RP Donovan, GH (reprint author), US Forest Serv, USDA, PNW Res Stn, 620 SW Main,Suite 400, Portland, OR 97205 USA. EM gdonovan@fs.fed.us RI Liebhold, Andrew/C-1423-2008 OI Liebhold, Andrew/0000-0001-7427-6534 NR 37 TC 69 Z9 78 U1 9 U2 122 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0749-3797 J9 AM J PREV MED JI Am. J. Prev. Med. PD FEB PY 2013 VL 44 IS 2 BP 139 EP 145 DI 10.1016/j.amepre.2012.09.066 PG 7 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA 077ZJ UT WOS:000314067600009 PM 23332329 ER PT J AU Iovanna, R Vance, C AF Iovanna, Richard Vance, Colin TI Land conversion and market equilibrium: insights from a simulated landscape SO ANNALS OF REGIONAL SCIENCE LA English DT Article ID AGENT-BASED MODEL; URBAN SPRAWL; DEFORESTATION; BEHAVIOR; PRICES; COVER; ROADS AB We specify a system of equations that fully reflects the supply and demand sides of the market for agricultural open space at equilibrium. Although simple, the system is exceedingly flexible and allows for household and parcel heterogeneity. We derive an empirical model directly from the structural equations and contrast this using a simulated landscape with the econometric specification most often found in the literature. We then show how the model can be used to project land-use change into the future and for policy simulation. Finally, we use the model to examine the impact of common land conservation policies in Europe. C1 [Iovanna, Richard] USDA, Farm Serv Agcy, Washington, DC 20250 USA. [Vance, Colin] RWI, D-45128 Essen, Germany. [Vance, Colin] Jacobs Univ Bremen, D-28759 Bremen, Germany. RP Vance, C (reprint author), RWI, Hohenzollernstr 1-3, D-45128 Essen, Germany. EM Rich.Iovanna@wdc.usda.gov; vance@rwi-essen.de NR 24 TC 1 Z9 1 U1 3 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0570-1864 EI 1432-0592 J9 ANN REGIONAL SCI JI Ann. Reg. Sci. PD FEB PY 2013 VL 50 IS 1 BP 169 EP 184 DI 10.1007/s00168-011-0486-9 PG 16 WC Economics; Environmental Studies; Geography SC Business & Economics; Environmental Sciences & Ecology; Geography GA 081FJ UT WOS:000314302600008 ER PT J AU Ceglia, L Harris, SS AF Ceglia, Lisa Harris, Susan S. TI Vitamin D and Its Role in Skeletal Muscle SO CALCIFIED TISSUE INTERNATIONAL LA English DT Article DE Skeletal muscle; Vitamin D; Vitamin D receptor ID D-RECEPTOR GENE; RANDOMIZED-CONTROLLED-TRIAL; BONE-MINERAL DENSITY; SERUM 25-HYDROXYVITAMIN D; PROTEIN-KINASE-C; D ENDOCRINE SYSTEM; D SUPPLEMENTATION; PHYSICAL FUNCTION; OLDER-ADULTS; 1,25-DIHYDROXYVITAMIN D-3 AB This review discusses the clinical and laboratory studies that have examined a role of vitamin D in skeletal muscle. Many observational studies, mainly in older populations, indicate that vitamin D status is positively associated with muscle strength and physical performance and inversely associated with risk of falling. Clinical trials of vitamin D supplementation in older adults with low vitamin D status mostly report improvements in muscle performance and reductions in falls. The underlying mechanisms are probably both indirect via calcium and phosphate and direct via activation of the vitamin D receptor (VDR) on muscle cells by 1,25-dihydroxyvitamin D [1,25(OH)(2)D]. VDR activation at the genomic level regulates transcription of genes involved in calcium handling and muscle cell differentiation and proliferation. A putative membrane-associated VDR activates intracellular signaling pathways also involved in calcium handling and signaling and myogenesis. Additional evidence comes from VDR knockout mouse models with abnormal muscle morphology and physical function, and VDR polymorphisms which are associated with differences in muscle strength. Recent identification of CYP27B1 bioactivity in skeletal muscle cells and in regenerating adult mouse muscle lends support to the direct action of both 25-hydroxyvitamin D and 1,25(OH)(2)D in muscle. Despite these research advances, many questions remain. Further research is needed to fully characterize molecular mechanisms of vitamin D action on muscle cells downstream of the VDR, describe the effects on muscle morphology and contractility, and determine whether these molecular and cellular effects translate into clinical improvements in physical function. C1 [Ceglia, Lisa] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA 02111 USA. [Ceglia, Lisa; Harris, Susan S.] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Ceglia, L (reprint author), Tufts Med Ctr, Div Endocrinol Diabet & Metab, 800 Washington St,Box 268, Boston, MA 02111 USA. EM lisa.ceglia@tufts.edu FU U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This material is based upon work supported by the U.S. Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 129 TC 61 Z9 62 U1 1 U2 31 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0171-967X J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD FEB PY 2013 VL 92 IS 2 SI SI BP 151 EP 162 DI 10.1007/s00223-012-9645-y PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 077RA UT WOS:000314043900008 PM 22968766 ER PT J AU Dawson-Hughes, B AF Dawson-Hughes, Bess TI What is the Optimal Dietary Intake of Vitamin D for Reducing Fracture Risk? SO CALCIFIED TISSUE INTERNATIONAL LA English DT Article DE Vitamin D; 25OHD; Fracture; Bone mineral density; Parathyroid hormone ID RANDOMIZED-CONTROLLED-TRIAL; SERUM PARATHYROID-HORMONE; BONE-MINERAL DENSITY; PLACEBO-CONTROLLED TRIAL; D SUPPLEMENTATION; ELDERLY-WOMEN; 25-HYDROXYVITAMIN D; CALCIUM-ABSORPTION; SECONDARY HYPERPARATHYROIDISM; D INSUFFICIENCY AB Vitamin D has long been known to be important for bone health, but there is currently no consensus on the amount of vitamin D needed or how it varies with age, race/ethnicity, body size, absorption efficiency, season, and other factors. This review describes the effects of vitamin D on calcium absorption, parathyroid hormone levels, and changes in bone mineral density briefly and focuses in more detail on the results and interpretation of double-blind randomized controlled trials with fracture outcomes in older adults. Based on these trials, 400 IU/day, bringing 25-hydroxyvitamin D (25OHD) levels to 60-65 nmol/L, is not effective at lowering fracture risk in community-dwelling elders or in elders with prior fractures. Several large trials indicate that doses in the range of 700-1,000 IU/day can lower fracture risk by similar to 20 %. From these trials, it appears that a 25OHD level of 65 nmol/L is needed to reduce nonvertebral fracture risk and 75 nmol/L may be needed to lower hip fracture risk. Trials testing additional doses of vitamin D in different segments of the population are needed. C1 Tufts Univ, Jean Mayer United States Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Dawson-Hughes, B (reprint author), Tufts Univ, Jean Mayer United States Dept Agr, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM bess.dawson-hughes@tufts.edu FU U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This material is based on work supported by the U.S. Department of Agriculture, Agricultural Research Service, under agreement 58-1950-7-707. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture, the Centers for Disease Control and Prevention, or the U.S. Department of Health and Human Services. NR 46 TC 9 Z9 9 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0171-967X EI 1432-0827 J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD FEB PY 2013 VL 92 IS 2 SI SI BP 184 EP 190 DI 10.1007/s00223-012-9606-5 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 077RA UT WOS:000314043900011 PM 22592290 ER PT J AU Erpul, G Gabriels, D Norton, LD Flanagan, DC Huang, CH Visser, SM AF Erpul, Gunay Gabriels, Donald Norton, L. Darrell Flanagan, Dennis C. Huang, Chi-hua Visser, Saskia M. TI Mechanics of interrill erosion with wind-driven rain SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE WEPP; interrill erosion; wind-driven rain; raindrop impact velocity vector; kinetic energy flux ID SEDIMENT TRANSPORT RATE; SPLASH-SALTATION; WATER EROSION; SOIL-EROSION; SAND DETACHMENT; PREDICTION; MODEL; RAINDROPS; INCIDENT; TUNNEL AB The vector physics of wind-driven rain (WDR) differs from that of wind-free rain, and the interrill soil detachment equations in the Water Erosion Prediction Project (WEPP) model were not originally developed to deal with this phenomenon. This article provides an evaluation of the performance of the interrill component of the WEPP model for WDR events. The interrill delivery rates were measured in the wind tunnel facility of the International Center for Eremology (ICE), Ghent University, Belgium with an experimental setup to study different raindrop impact velocity vectors. Synchronized wind and rain simulations with wind velocities of 6, 10 and 14 m s1 were applied to a test surface placed on windward and leeward slopes of 7, 15 and 20%. Since both rainfall intensity and raindrop impact velocity varied greatly depending on differences in the horizontal wind velocity under WDRs, the resultant kinetic energy flux (KEr, in J m2 s1) was initially used in place of the WEPP model intensity term in order to incorporate the effect of wind on impact velocity and frequency of raindrops. However, our results showed only minor improvement in the model predictions. For all research data, the model Coefficients of Determination (r2) were 0.63 and 0.71, when using the WEPP and the KEr approaches, respectively. Alternately, integrating the angle of rain incidence into the model by vectorally partitioning normal kinetic energy flux (KErn, in J m2 s1) from the KEr greatly improved the model's ability to estimate the interrill sediment delivery rates (r2 = 0.91). This finding suggested that along with the fall trajectory of wind-driven raindrops with a given frequency, raindrop velocity and direction at the point of impact onto the soil surface provided sufficient physical information to improve WEPP sediment delivery rate predictions under WDR. Copyright (C) 2012 John Wiley & Sons, Ltd. C1 [Erpul, Gunay] Ankara Univ, Fac Agr, Dept Soil Sci, TR-06110 Ankara, Turkey. [Erpul, Gunay] Ankara Univ, Earth Sci Applicat & Res Ctr, TR-06100 Ankara, Turkey. [Gabriels, Donald] Univ Ghent, Dept Soil Management, B-9000 Ghent, Belgium. [Gabriels, Donald] Univ Ghent, UNESCO Chair Eremol, B-9000 Ghent, Belgium. [Norton, L. Darrell; Flanagan, Dennis C.; Huang, Chi-hua] Purdue Univ, ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Visser, Saskia M.] Alterra, Soil Phys & Land Use Team, Wageningen Ur, Netherlands. RP Erpul, G (reprint author), Ankara Univ, Fac Agr, Dept Soil Sci, TR-06110 Ankara, Turkey. EM erpul@agri.ankara.edu.tr FU Abdus Salam International Center for Theoretical Physics (ICTP), Trieste, Italy FX Dr G. Erpul thanks the Abdus Salam International Center for Theoretical Physics (ICTP), Trieste, Italy for providing financial support for his visits (as a Regular Associate) to both the ICTP and the partner institute, UNESCO Chair on Eremology, Ghent University, Belgium. NR 58 TC 1 Z9 1 U1 2 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD FEB PY 2013 VL 38 IS 2 BP 160 EP 168 DI 10.1002/esp.3280 PG 9 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 076TP UT WOS:000313981200005 ER PT J AU Sorte, CJB Ibanez, I Blumenthal, DM Molinari, NA Miller, LP Grosholz, ED Diez, JM D'Antonio, CM Olden, JD Jones, SJ Dukes, JS AF Sorte, Cascade J. B. Ibanez, Ines Blumenthal, Dana M. Molinari, Nicole A. Miller, Luke P. Grosholz, Edwin D. Diez, Jeffrey M. D'Antonio, Carla M. Olden, Julian D. Jones, Sierra J. Dukes, Jeffrey S. TI Poised to prosper? A cross-system comparison of climate change effects on native and non-native species performance SO ECOLOGY LETTERS LA English DT Review DE Climate change; CO2; effect size; forecasting; global warming; invasive species; meta-analysis; performance; precipitation; resource utilisation ID ALIEN PLANT INVASIONS; GLOBAL CHANGE; BIOLOGICAL INVASIONS; ENVIRONMENTAL-MANAGEMENT; METAANALYSIS; RESPONSES; COMMUNITY; SUCCESS; CONSERVATION; IMPACTS AB Climate change and biological invasions are primary threats to global biodiversity that may interact in the future. To date, the hypothesis that climate change will favour non-native species has been examined exclusively through local comparisons of single or few species. Here, we take a meta-analytical approach to broadly evaluate whether non-native species are poised to respond more positively than native species to future climatic conditions. We compiled a database of studies in aquatic and terrestrial ecosystems that reported performance measures of non-native (157 species) and co-occurring native species (204 species) under different temperature, CO2 and precipitation conditions. Our analyses revealed that in terrestrial (primarily plant) systems, native and non-native species responded similarly to environmental changes. By contrast, in aquatic (primarily animal) systems, increases in temperature and CO2 largely inhibited native species. There was a general trend towards stronger responses among non-native species, including enhanced positive responses to more favourable conditions and stronger negative responses to less favourable conditions. As climate change proceeds, aquatic systems may be particularly vulnerable to invasion. Across systems, there could be a higher risk of invasion at sites becoming more climatically hospitable, whereas sites shifting towards harsher conditions may become more resistant to invasions. C1 [Sorte, Cascade J. B.] Univ Massachusetts, Dept Environm Earth & Ocean Sci, Boston, MA 02125 USA. [Ibanez, Ines] Univ Michigan, Sch Nat Resources, Ann Arbor, MI 48109 USA. [Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80522 USA. [Molinari, Nicole A.; D'Antonio, Carla M.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Miller, Luke P.] Stanford Univ, Hopkins Marine Stn, Pacific Grove, CA 93950 USA. [Grosholz, Edwin D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Diez, Jeffrey M.] ETH, Inst Integrat Biol, Zurich, Switzerland. [Olden, Julian D.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Jones, Sierra J.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. RP Sorte, CJB (reprint author), Univ Massachusetts, Dept Environm Earth & Ocean Sci, Harbor Campus, Boston, MA 02125 USA. EM cjsorte@ucdavis.edu RI Dukes, Jeffrey/C-9765-2009; Miller, Luke/B-8992-2009; OI Dukes, Jeffrey/0000-0001-9482-7743; Miller, Luke/0000-0002-2009-6981; Olden, Julian/0000-0003-2143-1187 FU Department of Energy [DOE-ER64982]; National Center for Ecological Analysis and Synthesis; National Science Foundation [EF-0553768]; University of California, Santa Barbara; State of California FX We thank J. Kray and J. Heisler-White for assistance with data extraction. Discussions with B. Bradley, R. Early, J. Lawler and C. Lortie were especially helpful to the development of this project. We are grateful to M. Bracken for analytical assistance and comments. Funding for C. J. B. S. was provided by the Department of Energy (DOE-ER64982 for 'Establishment of the Northeast Coastal Watershed Geospatial Data Network'). This study was conducted as part of the Climate & Invasions Working Group with support provided by the National Center for Ecological Analysis and Synthesis, a Center funded by the National Science Foundation (grant #EF-0553768), the University of California, Santa Barbara and the State of California. NR 72 TC 66 Z9 73 U1 25 U2 405 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X J9 ECOL LETT JI Ecol. Lett. PD FEB PY 2013 VL 16 IS 2 BP 261 EP 270 DI 10.1111/ele.12017 PG 10 WC Ecology SC Environmental Sciences & Ecology GA 073DF UT WOS:000313722900017 PM 23062213 ER PT J AU Zhang, JE Yu, JY Ouyang, Y Xu, HQ AF Zhang, Jia'en Yu, Jiayu Ouyang, Ying Xu, Huaqin TI Responses of earthworm to aluminum toxicity in latosol SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Al toxicity; Earthworm; Latosol ID ACID-RAIN; TIME AB Excess aluminum (Al) in soils due to acid rain leaching is toxic to water resources and harmful to soil organisms and plants. This study investigated adverse impacts of Al levels upon earthworms (Eisenia fetida) from the latosol (acidic red soil). Laboratory experiments were performed to examine the survival and avoidance of earthworms from high Al concentrations and investigate the response of earthworms upon Al toxicity at seven different Al concentrations that ranged from 0 to 300 mg kg(-1) over a 28-day period. Our study showed that the rate of the earthworm survival was 100 % within the first 7 days and decreased as time elapsed, especially for the Al concentrations at 200 and 300 mg kg(-1). A very good linear correlation existed between the earthworm avoidance and the soil Al concentration. There was no Al toxicity to earthworms with the Al concentration a parts per thousand currency sign50 mg kg(-1), and the toxicity started with the Al concentration a parts per thousand yen100 mg kg(-1). Low Al concentration (i.e., < 50 mg kg(-1)) enhanced the growth of the earthworms, while high Al concentration (> 100 mg kg(-1)) retarded the growth of the earthworms. The weight of earthworms and the uptake of Al by earthworms increased with the Al concentrations from 0 to 50 mg kg(-1) and decreased with the Al concentrations from 50 to 300 mg kg(-1). The protein content in the earthworms decreased with the Al concentrations from 0 to 100 mg kg(-1) and increased from 100 to 300 mg kg(-1). In contrast, the catalase (CAT) and superoxide dismutase (SOD) activities in the earthworms increased with the Al concentrations from 0 to 100 mg kg(-1) and decreased from 100 to 300 mg kg(-1). The highest CAT and SOD activities and lowest protein content were found at the Al concentration of 100 mg kg(-1). Results suggest that a high level of Al content in latosol was harmful to earthworms. C1 [Zhang, Jia'en; Yu, Jiayu; Xu, Huaqin] S China Agr Univ, Dept Ecol, Guangzhou, Guangdong, Peoples R China. [Ouyang, Ying] US Forest Serv, USDA, Mississippi State, MS 39762 USA. RP Zhang, JE (reprint author), S China Agr Univ, Dept Ecol, Wushan Rd, Guangzhou, Guangdong, Peoples R China. EM jeanzh@scau.edu.cn; youyang@fs.fed.us FU National Natural Science Foundation of China [40871118]; National Natural Science Foundation of Guangdong Province, China [8151064201000048, 9451064201003801, S2011010001570]; China Post-doctoral Special Fund [201003355] FX The study was supported by the National Natural Science Foundation of China (no. 40871118), National Natural Science Foundation of Guangdong Province, China (no. 8151064201000048, no. 9451064201003801, and no. S2011010001570), and China Post-doctoral Special Fund (no. 201003355). NR 26 TC 7 Z9 8 U1 0 U2 47 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD FEB PY 2013 VL 20 IS 2 BP 1135 EP 1141 DI 10.1007/s11356-012-0969-y PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 077YK UT WOS:000314065100050 PM 22645004 ER PT J AU Bartholome, LT Peterson, RE Raatz, SK Raymond, NC AF Bartholome, Lindsay T. Peterson, Roseann E. Raatz, Susan K. Raymond, Nancy C. TI A comparison of the accuracy of self-reported intake with measured intake of a laboratory overeating episode in overweight and obese women with and without binge eating disorder SO EUROPEAN JOURNAL OF NUTRITION LA English DT Article DE Binge eating disorder; Obesity; Food intake; Meal patterning; Dietary recall; Underreporting ID TEST MEAL INTAKE; ENERGY-INTAKE; FOOD SELECTION; EXPENDITURE; VALIDITY; PATTERNS; EATERS AB Research has demonstrated significant underreporting of food intake in obese individuals with and without binge eating disorder (BED). An improved understanding of the accuracy of self-reported food intake is central to diagnosis of eating disorders and monitoring response to treatment. The purpose was to: (1) confirm those with BED consume significantly more kilocalories (kcal) than overweight/obese controls when instructed to overeat in the laboratory and (2) compare dietary recall data with measured intake. Fifteen women fulfilling BED criteria and 17 controls participated in an overeating episode and completed a 24-h dietary recall. BED participants consumed significantly more kilocalories according to both methodologies. The BED group self-reported 90% of the measured intake compared to 98% for the control group. Mean differences between the methods indicated that on average both groups underreported intake; however, the mean difference between methods was significantly greater in the BED group. Findings confirm that those with BED consume significantly more than controls during a laboratory binge and controls tended to be more accurate in recalling their intake 24 h later. C1 [Bartholome, Lindsay T.; Raymond, Nancy C.] Univ Minnesota, Sch Med, Dept Psychiat, Minneapolis, MN 55454 USA. [Peterson, Roseann E.] Virginia Commonwealth Univ, Virginia Inst Psychiat & Behav Genet, Dept Human & Mol Genet, Richmond, VA 23220 USA. [Raatz, Susan K.] USDA, Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, Minneapolis, MN 55455 USA. RP Raymond, NC (reprint author), Univ Minnesota, Sch Med, Dept Psychiat, F282-2A W,2450 Riverside Ave, Minneapolis, MN 55454 USA. EM raymo002@umn.edu FU National Institutes of Health [R01 MH 060199, MO1-RR00400]; Minnesota Obesity Center [P30 DK 60456]; National Institute on Drug Abuse [DA-26119] FX This study was funded by a National Institutes of Health Grant (R01 MH 060199, MO1-RR00400), supported in part by the Minnesota Obesity Center Grant (P30 DK 60456) and National Institute on Drug Abuse (DA-26119). The authors wish to thank Jennifer Hommerding for her assistance with data collection. NR 27 TC 8 Z9 10 U1 0 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-6207 J9 EUR J NUTR JI Eur. J. Nutr. PD FEB PY 2013 VL 52 IS 1 BP 193 EP 202 DI 10.1007/s00394-012-0302-z PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 074KT UT WOS:000313812100019 PM 22302613 ER PT J AU Campion, B Glahn, RP Tava, A Perrone, D Doria, E Sparvoli, F Cecotti, R Dani, V Nielsen, E AF Campion, Bruno Glahn, Raymond P. Tava, Aldo Perrone, Domenico Doria, Enrico Sparvoli, Francesca Cecotti, Roberto Dani, Valeria Nielsen, Erik TI Genetic reduction of antinutrients in common bean (Phaseolus vulgaris L.) seed, increases nutrients and in vitro iron bioavailability without depressing main agronomic traits SO FIELD CROPS RESEARCH LA English DT Article DE ADF; ADL; Cellulose; Crude proteins; Ferritin; Free phosphorus; Hemicellulose; Lectins; Lignin; NDF; Phytic acid; Saponins; Tannins; Total phenolics; Trypsin inhibitors; Zinc ID PHYTIC-ACID MUTANT; HUMAN-NUTRITION; LOW-PHYTATE; SAPONINS; QUALITY; CANCER; MODEL; RED; POLYPHENOLICS; MALNUTRITION AB In common bean, lectins, phytic acid, polyphenols and tannins exert major antinutritional effects when grains are consumed as a staple food. Reduced iron and zinc absorption, low protein digestibility and high toxicity at the intestinal level are the causes of their antinutritional effect. To improve grain nutritional characteristics, the "low phytic acid" (lpa) trait recently obtained in bean and carried by the bean mutant lpa-280-10, was introgressed into different lectin-free (If) lines, a few of which were white-seeded. The "white seed coat" (wsc) trait is correlated with a reduced amount of tannins and polyphenols in bean seed, and thus higher Fe bioavailability. Lf + lpa bean lines producing colored and white seeds, were developed. Three of these lines were submitted to a first field performance test carried out in two Italian locations, and two of them to biochemical analyses that evaluated fourteen nutritional parameters. Seedling emergence and grain yield of lf + lpa beans were statistically comparable to those of wild type cultivars, confirming the absence of major agronomic defects associated with the lpa trait. The presence of the three genetic traits If lpa and wsc in the same genetic background leads to a significant increase of the content of important nutrients such as crude proteins, total zinc, free phosphorus, and, in part, total iron. Iron bioavailability (as measured in vitro via a Caco-2 cell model) in lf + lpa brown and black seeds, was not significantly different from that surveyed in the wild type colored parents, while, it was on average twelve times higher in lf + lpa white bean seeds. Up to now, the white-seeded lf + lpa beans seem thus to be the only materials having really improved nutritional qualities. (c) 2012 Elsevier B.V. All rights reserved. C1 [Campion, Bruno; Dani, Valeria] Consiglio Ric & Sperimentaz Agr, Unita Ric Orticoltura, I-26836 Lodi, Italy. [Glahn, Raymond P.] USDA ARS, Plant Soil & Nutr Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Tava, Aldo; Cecotti, Roberto] Consiglio Ric & Sperimentaz Agr, Ctr Ric Prod Foraggere & Lattiero Casearie, I-26900 Lodi, Italy. [Perrone, Domenico] Consiglio Ric & Sperimentaz Agr, Ctr Ric Orticoltura, Grp Battipaglia, I-84091 Salerno, Italy. [Doria, Enrico; Nielsen, Erik] Univ Pavia, Dipartimento Biol & Biotecnol L Spallanzani, I-27100 Pavia, Italy. [Sparvoli, Francesca] CNR, Ist Biol & Biotecnol Agr, I-20133 Milan, Italy. RP Campion, B (reprint author), Consiglio Ric & Sperimentaz Agr, Unita Ric Orticoltura, Via Paullese 28, I-26836 Lodi, Italy. EM bruno.campion@alice.it RI Sparvoli, Francesca/G-8372-2015 OI Sparvoli, Francesca/0000-0002-3304-7548 FU Ministry of Agricultural Alimentary and Forest Politics; C.I.P.E.; Regione Lombardia/CNR FX Technical support by Agata Ursino, Barbara Pintus and Rommel Ocampo Romero Ivan is acknowledged. We also acknowledge Roberto Pilu for soil analyses. This research was partially supported by Ministry of Agricultural Alimentary and Forest Politics with funds released by C.I.P.E. (Resolution 17/2003) to B.C. and F.S. and by Regione Lombardia/CNR agreement, project 2 to F.S. NR 64 TC 8 Z9 9 U1 3 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 1 PY 2013 VL 141 BP 27 EP 37 DI 10.1016/j.fcr.2012.10.015 PG 11 WC Agronomy SC Agriculture GA 083BI UT WOS:000314437300004 ER PT J AU Huwe, JK Archer, JC AF Huwe, J. K. Archer, J. C. TI Dioxin congener patterns in commercial catfish from the United States and the indication of mineral clays as the potential source SO FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT LA English DT Article DE dioxins; catfish; feeds; kaolin clay; congener pattern ID DIBENZO-P-DIOXINS; FARM-RAISED CATFISH; CONTAMINATION; HEALTH; BIPHENYLS; PCBS; PCDD; USA AB Since 1991 the US Department of Agriculture (USDA) has conducted annual surveys of pesticide residues in foods under the Agricultural Marketing Service's Pesticide Data Program (PDP). To assess chemical residues in domestically marketed catfish products, 1479 catfish samples were collected during the 20082010 PDPs. A subset of 202 samples was analysed for 17 toxic polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs). The average pattern of the individual PCDD/F congener concentrations in the catfish was rather unique in that it had almost no measurable amounts of polychlorinated dibenzofurans (PCDFs), but all PCDDs were present. This pattern was more dominant in the domestically produced catfish products than in the imported products (China/Taiwan). Comparison of the pattern to known sources of PCDD/Fs showed strong similarities to the pattern of PCDD/Fs found in kaolin clays which have often been used as anti-caking agents in animal feeds. To investigate whether catfish feeds may be the source of the PCDD/Fs found in the catfish, archived catfish feed data from a US Food and Drug Administration (USFDA) database were examined. In 61 out of 112 feed samples, the PCDD concentrations were 50 times higher than the PCDF concentrations and resembled the pattern found in the catfish products and in clays mined in the south-eastern United States. Although the source of PCDD/Fs in domestically marketed catfish products cannot be definitively established, mined clay products used in feeds should be considered a likely source and, given the wide concentration range of PCDD/Fs that has been found in clays, a critical control point for PCDD/Fs entrance to the food supply. C1 [Huwe, J. K.] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Archer, J. C.] US FDA, Arkansas Reg Lab, Jefferson, AR 72079 USA. RP Huwe, JK (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM Janice.Huwe@ars.usda.gov FU FSIS-ARS [60-5442-9-0476] FX The authors would like to acknowledge Kristin McDonald and Jean Picard for technical assistance with sample purification, and Margaret Lorentzsen and Grant Harrington for HRGC-HRMS analysis. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA) and the US Food and Drug Administration (USFDA). The USDA and USFDA are equal opportunity providers and employers. Funding for this exploratory assessment study was provided through FSIS-ARS Interagency Agreement #60-5442-9-0476. NR 26 TC 1 Z9 1 U1 0 U2 26 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1944-0049 J9 FOOD ADDIT CONTAM A JI Food Addit. Contam. Part A-Chem. PD FEB 1 PY 2013 VL 30 IS 2 BP 331 EP 338 DI 10.1080/19440049.2012.745199 PG 8 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA 072KW UT WOS:000313670900014 PM 23234292 ER PT J AU Hurisso, TT Davis, JG Brummer, JE Stromberger, ME Mikha, MM Haddix, ML Booher, MR Paul, EA AF Hurisso, Tunsisa T. Davis, Jessica G. Brummer, Joe E. Stromberger, Mary E. Mikha, Maysoon M. Haddix, Michelle L. Booher, Matthew R. Paul, Eldor A. TI Rapid changes in microbial biomass and aggregate size distribution in response to changes in organic matter management in grass pasture SO GEODERMA LA English DT Article DE Composted dairy manure; Cool-season perennial grasses; Aggregate stability; Microbial biomass C; Soil organic C ID NO-TILLAGE; SOIL AGGREGATION; MANURE APPLICATIONS; CULTIVATED SOILS; WIND ERODIBILITY; SOUTHERN ALBERTA; LOAM SOIL; CARBON; DYNAMICS; CROP AB Adding high quantities of organic matter can increase carbon (C) inputs to soil and help maintain soil structure. This study investigated short-term effects of application of different levels of composted dairy manure (CDM) versus interseeding a legume into grass pasture on aggregate stability and soil C and nitrogen (N) contents. CDM was added to a mixture of perennial grasses at 22.4, 33.6 or 44.8 Mg ha(-1). A grass-legume treatment was established by interseeding alfalfa (Medicago saliva) into the grass mixture. A no-input control was sampled as a reference. Soils (0-5 and 5-15 cm) were sampled approximately 1.5 years after study implementation and wet sieved to obtain four aggregate size classes: large macroaggregates (>2000 mu m), small macroaggregates (250-2000 mu m), microaggregates (53-250 mu m) and silt and clay fraction (<53 mu m). Significant CDM influences were found in the 5-15 cm depth. The addition of 44.8 Mg CDM ha(-1) and alfalfa resulted in higher proportions of macroaggregates (>250 mu m) and mean weight diameter (MWD) than CDM added at 22.4 or 33.6 Mg ha(-1). Addition of CDM at low dose rate and alfalfa did not affect total soil or aggregate-associated organic C or N. However, addition of CDM at 44.8 Mg ha(-1) and alfalfa resulted in higher total soil microbial biomass C and N compared to CDM added at 22.4 and 33.6 Mg ha(-1). Large macroaggregates were found to be positively correlated with total soil microbial biomass C (R = 0.81, p = 0.002). In conclusion, compared to a low application rate of CDM, addition of a high application rate of CDM or alfalfa interseeding resulted in higher total soil microbial biomass C and N and macroaggregates, and these changes in microbial biomass and aggregation occurred very rapidly. (c) 2012 Elsevier B.V. All rights reserved. C1 [Hurisso, Tunsisa T.; Davis, Jessica G.; Brummer, Joe E.; Stromberger, Mary E.; Booher, Matthew R.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Mikha, Maysoon M.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Haddix, Michelle L.; Paul, Eldor A.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Davis, JG (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM Jessica.davis@colostate.edu RI Davis, Jessica/C-1903-2013; Stromberger, Mary/C-3070-2013; OI Stromberger, Mary/0000-0002-5862-2932; Haddix, Michelle/0000-0003-0984-0404 FU Aurora Organic Dairy FX This study was funded by Aurora Organic Dairy. We are also grateful to Michael Herder, Dani Boneta Herrero and Dan McCue for helping with field sampling and for their many hours of wet sieving. We appreciate the assistance of Dan Ruess and Colin Pinney in the NREL during C and N analyses. Many thanks also to James ZumBrunnen for the guidance with the statistical analyses. NR 49 TC 9 Z9 9 U1 4 U2 80 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 J9 GEODERMA JI Geoderma PD FEB PY 2013 VL 193 BP 68 EP 75 DI 10.1016/j.geoderma.2012.10.016 PG 8 WC Soil Science SC Agriculture GA 079SV UT WOS:000314193400007 ER PT J AU David, GCL Legleiter, CJ Wohl, E Yochum, SE AF David, Gabrielle C. L. Legleiter, Carl J. Wohl, Ellen Yochum, Steven E. TI Characterizing spatial variability in velocity and turbulence intensity using 3-D acoustic Doppler velocimeter data in a plane-bed reach of East St. Louis Creek, Colorado, USA SO GEOMORPHOLOGY LA English DT Article DE Stage-dependent flow structure; Semivariogram; Stochastic hydraulics; Plane-bed; Mountain streams; Boulder-bed river ID FLOW RESISTANCE; HEADWATER STREAMS; RECORD LENGTH; CHANNEL FLOW; POOL CHANNEL; RIVER; FIELD; HYDRAULICS; TOPOGRAPHY; PREDICTION AB We investigated the influence on flow resistance of flow structure and turbulence at the reach scale in a mountain channel using 3-D velocity measurements and geostatistical analysis to understand the complexity of the flow structure in a reach with limited bed irregularities. The increase in flow resistance at low flows in a plane-bed reach was not fully explained by grain resistance, therefore detailed 3-D velocity measurements were made to investigate spatial variability in velocity and turbulence components and potential controls on flow resistance. One plane-bed reach was surveyed over two stages in Fraser Experimental Forest, Colorado, using a combination of a total station, LiDAR (Light Detection and Ranging), and a SonTek Flowtracker handheld ADV (acoustic Doppler velocimeter). LiDAR was used to capture bank and channel geometry at low flows, whereas the water surface and bed data were collected with the total station at all flows. We used the standard deviation of bed elevation (sigma(b)) within a moving window as an index of roughness height (k(s)) and calculated the relative submergence of the bed at different stages as h/k(s), where h is the local flow depth. ADV measurements were collected on a grid with a 0.3 m to 0.5 m spacing. Geostatistical analysis of the velocity data indicated that the flow was highly three-dimensional and varied based on stage, demonstrating that even small irregularities in the bed have a significant influence on the flow characteristics. The streamwise component was the largest at both low and high flow, but varied more throughout the reach at low flow. At high flow, the greatest streamwise velocities were located within the thalweg. Areas of upwelling and downwelling also varied based on stage, with this component being strongly influenced by small changes in the morphology at high flow, and by protuberant grains at low flows. The cross-stream velocity and turbulence components were controlled by the flow structure and less by the roughness of the bed. The turbulence intensity is significant when considering hydraulics for predicting sediment transport and for habitat assessment. The plane-bed flow patterns were compared to results from a pool-riffle reach in the North Fork Cache La Poudre River, a step-pool reach in East St. Louis Creek, and a step-pool reach in Italy on the Rio Cordon. The comparison of the 3-D velocities among channel types showed that the plane-bed reach has higher streamwise velocities, but similar values of cross-stream and transverse velocities. Streamwise turbulence intensities were similar in both the plane-bed and step-pool reach in East St. Louis Creek. The analysis revealed that the connection between the flow characteristics and the channel was not only related to the gross morphology and location of larger clasts, but also to the development of shear layers from the convergence of flow. Therefore, it is essential to understand how water moving in one direction can alter the characteristics of another component of flow and how this interaction is connected to the bed morphology. (C) 2012 Elsevier B.V. All rights reserved. C1 [David, Gabrielle C. L.] Bowdoin Coll, Dept Earth & Oceanog Sci, Brunswick, ME 04011 USA. [Legleiter, Carl J.] Univ Wyoming, Dept Geog, Laramie, WY 82071 USA. [Wohl, Ellen] Colorado State Univ, Dept Geosci, Ft Collins, CO 80523 USA. [Yochum, Steven E.] Nat Resources Conservat Serv, USDA, Ft Collins, CO 80523 USA. RP David, GCL (reprint author), Bowdoin Coll, Dept Earth & Oceanog Sci, Brunswick, ME 04011 USA. EM gdavid@bowdoin.edu; Carl.Legleiter@uwyo.edu; ellenw@warnercnr.colostate.edu; steven.yochum@co.usda.gov OI Legleiter, Carl/0000-0003-0940-8013 FU National Science Foundation [EAR 0608918] FX This research was funded by the Hydrologic Sciences Program of the National Science Foundation (EAR 0608918). We would like to thank the USDA Forest Service Rocky Mountain Research Station and field assistants Mark Hussey, Dan Dolan, and Dan Cadol for providing logistical support. The manuscript was improved by comments from two anonymous reviewers. NR 54 TC 1 Z9 1 U1 1 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X J9 GEOMORPHOLOGY JI Geomorphology PD FEB 1 PY 2013 VL 183 SI SI BP 28 EP 44 DI 10.1016/j.geomorph.2012.07.026 PG 17 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 081NM UT WOS:000314328900004 ER PT J AU Fan, ZS McGuire, AD Turetsky, MR Harden, JW Waddington, JM Kane, ES AF Fan, Zhaosheng McGuire, Anthony David Turetsky, Merritt R. Harden, Jennifer W. Waddington, James Michael Kane, Evan S. TI The response of soil organic carbon of a rich fen peatland in interior Alaska to projected climate change SO GLOBAL CHANGE BIOLOGY LA English DT Article DE boreal; carbon; climate change; fen; methane; model; peatland; soil CO2 flux ID BLACK SPRUCE ECOSYSTEMS; WATER-TABLE LEVELS; METHANE EMISSIONS; NITROGEN MINERALIZATION; LABORATORY INCUBATIONS; NORTHERN PEATLANDS; DIOXIDE EXCHANGE; ROOT DYNAMICS; CO2; MODEL AB It is important to understand the fate of carbon in boreal peatland soils in response to climate change because a substantial change in release of this carbon as CO2 and CH4 could influence the climate system. The goal of this research was to synthesize the results of a field water table manipulation experiment conducted in a boreal rich fen into a process-based model to understand how soil organic carbon (SOC) of the rich fen might respond to projected climate change. This model, the peatland version of the dynamic organic soil Terrestrial Ecosystem Model (peatland DOS-TEM), was calibrated with data collected during 2005-2011 from the control treatment of a boreal rich fen in the Alaska Peatland Experiment (APEX). The performance of the model was validated with the experimental data measured from the raised and lowered water-table treatments of APEX during the same period. The model was then applied to simulate future SOC dynamics of the rich fen control site under various CO2 emission scenarios. The results across these emissions scenarios suggest that the rate of SOC sequestration in the rich fen will increase between year 2012 and 2061 because the effects of warming increase heterotrophic respiration less than they increase carbon inputs via production. However, after 2061, the rate of SOC sequestration will be weakened and, as a result, the rich fen will likely become a carbon source to the atmosphere between 2062 and 2099. During this period, the effects of projected warming increase respiration so that it is greater than carbon inputs via production. Although changes in precipitation alone had relatively little effect on the dynamics of SOC, changes in precipitation did interact with warming to influence SOC dynamics for some climate scenarios. C1 [Fan, Zhaosheng] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. [McGuire, Anthony David] Univ Alaska, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Turetsky, Merritt R.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 1G2, Canada. [Harden, Jennifer W.] US Geol Survey, Menlo Pk, CA 94025 USA. [Waddington, James Michael] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada. [Kane, Evan S.] ARS, USDA, No Res Stn, Houghton, MI 49931 USA. RP Fan, ZS (reprint author), Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. EM zfan2@alaska.edu RI Turetsky, Merritt/B-1255-2013 FU National Science Foundation for the APEX project (NSF) [DEB-0425328, DEB-0724514, DEB-0830997]; Bonanza Creek Long-Term Ecological Research program; NSF [DEB-0620579]; USDA Forest Service Pacific Northwest Research Grant [PNW01-JV11261952-231] FX This research was accomplished with support from the National Science Foundation for the APEX project (NSF; DEB-0425328, DEB-0724514, DEB-0830997), and support from the Bonanza Creek Long-Term Ecological Research program (funded jointly by NSF Grant DEB-0620579, and by USDA Forest Service Pacific Northwest Research Grant PNW01-JV11261952-231). We thank the numerous postdoctoral scientists, students, and technicians that have helped to collect data at the APEX sites, in particular Molly Chivers, Claire Treat, Gordon Shelter, Bill Cable, Ronnie Daanen, Collin Macheel, Amy Churchill, Nicole McConnell, Katie Shea, and Sara Klapstein. The authors also greatly appreciate the helpful comments from Steve Frolking (University of New Hampshire, USA) and two anonymous reviewers. NR 92 TC 16 Z9 17 U1 8 U2 144 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2013 VL 19 IS 2 BP 604 EP 620 DI 10.1111/gcb.12041 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 080CQ UT WOS:000314219200024 PM 23504796 ER PT J AU Butler, JE Sun, XZ Wertz, N Vincent, AL Zanella, EL Lager, KM AF Butler, John E. Sun, XiuZhu Wertz, Nancy Vincent, Amy L. Zanella, Eraldo L. Lager, Kelly M. TI Antibody repertoire development in fetal and neonatal piglets. XVI. Influenza stimulates adaptive immunity, class switch and diversification of the IgG repertoire encoded by downstream C gamma genes SO IMMUNOLOGY LA English DT Article DE antibody repertoire development; class switch recombination; IgG subclasses; swine influenza ID NATURAL ANTIBODIES; B-CELL; PREIMMUNE REPERTOIRE; HUMORAL IMMUNITY; NEWBORN PIGLETS; T-CELLS; ANTIGENS; RESPONSES; SYSTEM; MODEL AB Infection of germ-free isolator piglets with swine influenza (S-FLU) that generates dsRNA during replication causes elevation of immunoglobulins in serum and bronchoalveolar lavage, a very weak response to trinitrophenyl conjugates but an immune response to S-FLU. The increased immunoglobulin levels result mainly from the polyclonal activation of B cells during the infection, but model antigen exposure may contribute. The 10-fold increase in local and serum IgG accompanies a 10-fold decrease in the transcription of IgG3 in the trachealbronchial lymph nodes and in the ileal Peyer's patches. Infection results in class switch recombination to downstream C? genes, which diversify their repertoire; both features are diagnostic of adaptive immunity. Meanwhile the repertoires of IgM and IgG3 remain undiversified suggesting that they encode innate, natural antibodies. Whereas IgG3 may play an initial protective role, antibodies encoded by downstream C? genes with diversified repertoires are predicted to be most important in long-term protection against S-FLU. C1 [Butler, John E.; Sun, XiuZhu; Wertz, Nancy] Univ Iowa, Carver Coll Med, Dept Microbiol, Iowa City, IA 52240 USA. [Vincent, Amy L.; Lager, Kelly M.] USDA ARS, Virus & Prion Dis Unit, Natl Anim Dis Ctr, Ames, IA USA. [Zanella, Eraldo L.] Univ Passo Fundo, Curso Med Vet, Fundo, RS, Brazil. RP Butler, JE (reprint author), Univ Iowa, Carver Coll Med, Dept Microbiol, Iowa City, IA 52240 USA. EM john-butler@uiowa.edu FU NSF-IOS [0077237]; Biological Mimetics contract [NBCHC080090]; USDA-AFRI-NIFA/DHS [2010-39559-21860]; National Porkboard [07-210] FX This study was supported by NSF-IOS grant 0077237; Biological Mimetics contract NBCHC080090; USDA-AFRI-NIFA/DHS subcontract_#2010-39559-21860 and grant 07-210 from the National Porkboard. NR 46 TC 7 Z9 8 U1 1 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0019-2805 J9 IMMUNOLOGY JI Immunology PD FEB PY 2013 VL 138 IS 2 BP 134 EP 144 DI 10.1111/imm.12018 PG 11 WC Immunology SC Immunology GA 074TK UT WOS:000313836400006 PM 23320646 ER PT J AU Cordon, ARC Shirk, PD Duehl, AJ Evans, JD Teal, PEA AF Cordon, A. R. Cabrera Shirk, P. D. Duehl, A. J. Evans, J. D. Teal, P. E. A. TI Variable induction of vitellogenin genes in the varroa mite, Varroa destructor (Anderson & Trueman), by the honeybee, Apis mellifera L, host and its environment SO INSECT MOLECULAR BIOLOGY LA English DT Article DE ectoparasite; host-parasite interaction; reproduction; large lipid transfer gene superfamily; Apis mellifera ID JUVENILE-HORMONE-III; DEFORMED WING VIRUS; KASHMIR-BEE-VIRUS; PARASITIC MITE; JACOBSONI OUD; ECTOPARASITIC MITE; PERIPLANETA-AMERICANA; PRECURSOR PROTEINS; LEUCOPHAEA-MADERAE; NATURAL CONDITIONS AB Transcript levels of vitellogenins (Vgs) in the varroa mite, Varroa destructor (Anderson & Trueman), were variably induced by interactions between the developing honeybee, Apis mellifera L, as a food source and the capped honeybee cell environment. Transcripts for two Vgs of varroa mites were sequenced and putative Vg protein products characterized. Sequence analysis of VdVg1 and VdVg2 proteins showed that each had greater similarity with Vg1 and Vg2 proteins from ticks, respectively, than between themselves and were grouped separately by phylogenetic analyses. This suggests there was a duplication of the ancestral acarine Vg gene prior to the divergence of the mites and ticks. Low levels of transcript were detected in immature mites, males and phoretic females. Following cell invasion by phoretic females, VdVg1 and VdVg2 transcript levels were up-regulated after cell capping to a maximum at the time of partial cocoon formation by the honeybee. During oviposition the two transcripts were differentially expressed with higher levels of VdVg2 being observed. A bioassay based on assessing the transcript levels was established. Increases in VdVg1 and VdVg2 transcripts were induced experimentally in phoretic females when they were placed inside a cell containing an early metamorphosing last instar bee but not when exposed to the metamorphosing bee alone. The variable response of Vg expression to the food source as well as environmental cues within the capped cell demonstrates that perturbation of host-parasite interactions may provide avenues to disrupt the reproductive cycle of the varroa mites and prevent varroasis. C1 [Cordon, A. R. Cabrera; Shirk, P. D.; Duehl, A. J.; Teal, P. E. A.] USDA ARS CMAVE, Gainesville, FL 32608 USA. [Evans, J. D.] USDA ARS BRL, BARC E, Beltsville, MD USA. RP Shirk, PD (reprint author), USDA ARS CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM paul.shirk@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 NR 60 TC 13 Z9 13 U1 8 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD FEB PY 2013 VL 22 IS 1 BP 88 EP 103 DI 10.1111/imb.12006 PG 16 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 074KE UT WOS:000313810600009 ER PT J AU Zhang, XC AF Zhang, X-C TI Verifying a temporal disaggregation method for generating daily precipitation of potentially non-stationary climate change for site-specific impact assessment SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE climate change; temporal disaggregation; weather generator ID STOCHASTIC WEATHER GENERATOR; CIRCULATION MODEL OUTPUT; CHANGE SCENARIOS; LOCAL CLIMATE; UNITED-STATES; DOWNSCALING APPROACH; SIMULATED CLIMATE; CROP PRODUCTION; SOIL-EROSION; GCM OUTPUT AB Empirical statistical downscaling has been widely used to produce finer-resolution climate data. This approach, in general, is derived from an implicit stationarity assumption. This paper aims at proving a statistical method that is fully applicable of generating daily precipitation in non-stationary conditions using historical station data. Daily records at five Oklahoma stations were split into calibration and validation periods. Linear relationships between transition probabilities of wet-following-wet (Pw/w) and wet-following-dry (Pw/d) days and mean monthly precipitation were established by connecting the two endpoints (one for the 30 driest months and another for the 30 wettest months of the calibration period) for each calendar month, and were then used to interpolate Pw/w and Pw/d for the validation period. The mean and standard deviation of daily precipitation were estimated using the mean and standard deviation of monthly precipitation of the validation periods as well as the interpolated Pw/w and Pw/d. The adjusted parameters were used to generate daily series using a weather generator. Statistics of the disaggregated daily precipitation amounts and frequency, as well as dry/wet spell sequence, agreed with those of the observed values of the validation periods reasonably well. The disaggregation method preserved statistics of monthly precipitation amounts extremely well, demonstrating the validity of the method for temporal disaggregation of non-stationary climate. The accuracy of the presented method is within the weather generator's expected performance. Overall, a straight line connecting the two endpoints, implicitly incorporating non-stationarity of climate states, is adequate for interpolating Pw/w and Pw/d to any climatic conditions within the entire range. However, a linear regression including data points in between would generally improve the Pw/w and Pw/d interpolations slightly if long records (e.g. > 60 years) are available for estimating the intermediate points. Copyright (C) 2012 Royal Meteorological Society C1 USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Zhang, XC (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM John.Zhang@ars.usda.gov NR 57 TC 5 Z9 5 U1 1 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 J9 INT J CLIMATOL JI Int. J. Climatol. PD FEB PY 2013 VL 33 IS 2 BP 326 EP 342 DI 10.1002/joc.3425 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 073PB UT WOS:000313753900005 ER PT J AU Negi, SI Brautbar, A Virani, SS Anand, A Polisecki, E Asztalos, BF Ballantyne, CM Schaefer, EJ Jones, PH AF Negi, Smita I. Brautbar, Ariel Virani, Salim S. Anand, Aashish Polisecki, Eliana Asztalos, Bela F. Ballantyne, Christie M. Schaefer, Ernst J. Jones, Peter H. TI A novel mutation in the ABCA1 gene causing an atypical phenotype of Tangier disease SO JOURNAL OF CLINICAL LIPIDOLOGY LA English DT Article DE ABCA1 gene; Central nervous system; High-density lipoprotein; Peripheral neuropathy; Tangier disease ID HIGH-DENSITY-LIPOPROTEIN; CORONARY-HEART-DISEASE; HDL CHOLESTEROL LEVELS; CARDIOVASCULAR-DISEASE; APOA-I; DEFICIENCY; ATHEROSCLEROSIS; METABOLISM; FRAMINGHAM; EFFLUX AB Tangier disease is a rare autosomal-recessive disorder caused by mutation in the ATP binding cassette transporter 1 (ABCA1) gene. Typically, Tangier disease manifests with symptoms and signs resulting from the deposition of cholesteryl esters in nonadipose tissues; chiefly, in peripheral nerves leading to neuropathy and in reticulo-endothelial organs, such as liver, spleen, lymph nodes, and tonsils, causing their enlargement and discoloration. An association with early cardiovascular disease can be variable. We describe a patient with a unique phenotype of Tangier disease from a novel splice site mutation in the ABCA1 gene that is associated with a central nervous system presentation resembling multiple sclerosis, and the presence of premature atherosclerosis. (c) 2013 National Lipid Association. All rights reserved. C1 [Negi, Smita I.; Brautbar, Ariel; Virani, Salim S.; Ballantyne, Christie M.; Jones, Peter H.] Baylor Coll Med, Ctr Atherosclerosis & Lipoprot Res, Houston, TX 77030 USA. [Polisecki, Eliana; Asztalos, Bela F.; Schaefer, Ernst J.] Boston Heart Diagnost, Framingham, MA USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Anand, Aashish] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA. [Virani, Salim S.] Michael E DeBakey VA Med Ctr, Hlth Serv Res & Dev Ctr Excellence, Houston, TX USA. RP Jones, PH (reprint author), Baylor Coll Med, Ctr Atherosclerosis & Lipoprot Res, 6565 Fannin St,Suite B157, Houston, TX 77030 USA. EM jones@bcm.edu OI Virani, Salim/0000-0001-9541-6954 NR 22 TC 10 Z9 11 U1 0 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1933-2874 J9 J CLIN LIPIDOL JI J. Clin. Lipidol. PD FEB PY 2013 VL 7 IS 1 BP 82 EP 87 DI 10.1016/j.jacl.2012.09.004 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 083DW UT WOS:000314443900011 PM 23351586 ER PT J AU Courtney, SC Susta, L Gomez, D Hines, NL Pedersen, JC Brown, CC Miller, PJ Afonso, CL AF Courtney, Sean C. Susta, Leonardo Gomez, Dejelia Hines, Nichole L. Pedersen, Janice C. Brown, Corrie C. Miller, Patti J. Afonso, Claudio L. TI Highly Divergent Virulent Isolates of Newcastle Disease Virus from the Dominican Republic Are Members of a New Genotype That May Have Evolved Unnoticed for Over 2 Decades SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID STORKS CICONIA-CICONIA; PHYLOGENETIC ANALYSIS; COMPLETE GENOME; CLINICOPATHOLOGICAL CHARACTERIZATION; EVOLUTIONARY DISTANCE; RECENT OUTBREAKS; SEQUENCE; STRAINS; REPLICATION; CALIFORNIA AB A Newcastle disease virus (NDV) outbreak in chickens was reported in the Dominican Republic in 2008. The complete genome of this isolate, chicken/DominicanRepublic(JuanLopez)/499-31/2008 (NDV-DR499-31/08), and the fusion proteins of three other related viruses from the Dominican Republic and Mexico were sequenced and phylogenetically analyzed. Genetically, these four isolates were highly distinct from all other currently known isolates of NDV, and together, they fulfill the newly established criteria for inclusion as a novel genotype of NDV (genotype XVI). The lack of any reported isolation of viruses related to this group since 1986 suggests that virulent viruses of this genotype may have evolved unnoticed for 22 years. The NDV-DR499-31/08 isolate had an intracerebral pathogenicity index (ICPI) score of 1.88, and sequencing of the fusion cleavage site identified multiple basic amino acids and a phenylalanine at position 117, indicating this isolate to be virulent. These results were further confirmed by a clinicopathological assessment in vivo. In 4-week-old chickens, NDV-DR499-31/08 behaved as a velogenic viscerotropic strain with systemic virus distribution and severe necrohemorrhagic lesions targeting mainly the intestine and the lymphoid organs. The clear phylogenetic relationship between the 2008, 1986, and 1947 ancestral viruses suggests that virulent NDV strains may have evolved in unknown reservoirs in the Caribbean and surrounding regions and underlines the importance of continued and improved epidemiological surveillance strategies to detect NDV in wild-bird species and commercial poultry. C1 [Courtney, Sean C.; Susta, Leonardo; Miller, Patti J.; Afonso, Claudio L.] ARS, USDA, SE Poultry Res Lab, Athens, GA USA. [Gomez, Dejelia] Minist Agr, Santo Domingo, Dominican Rep. [Hines, Nichole L.; Pedersen, Janice C.] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Brown, Corrie C.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. RP Afonso, CL (reprint author), ARS, USDA, SE Poultry Res Lab, Athens, GA USA. EM claudio.afonso@ars.usda.gov FU USDA [CRIS 6612-32000-064-00D] FX This work was supported by USDA funding (CRIS 6612-32000-064-00D). NR 45 TC 23 Z9 27 U1 0 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 2013 VL 51 IS 2 BP 508 EP 517 DI 10.1128/JCM.02393-12 PG 10 WC Microbiology SC Microbiology GA 078OH UT WOS:000314108000019 PM 23196361 ER PT J AU Cariello, PF Wickes, BL Sutton, DA Castlebury, LA Levitz, SM Finberg, RW Thompson, EH Daly, JS AF Cariello, Paloma F. Wickes, Brian L. Sutton, Deanna A. Castlebury, Lisa A. Levitz, Stuart M. Finberg, Robert W. Thompson, Elizabeth H. Daly, Jennifer S. TI Phomopsis bougainvilleicola Prepatellar Bursitis in a Renal Transplant Recipient SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID IDENTIFICATION; VORICONAZOLE; THERAPY; FUNGI AB Prepatellar bursitis is typically a monomicrobial bacterial infection. A fungal cause is rarely identified. We describe a 61-year-old man who had received a renal transplant 21 months prior to presentation whose synovial fluid and surgical specimens grew Phomopsis bougainvilleicola, a pycnidial coelomycete. C1 [Cariello, Paloma F.; Levitz, Stuart M.; Finberg, Robert W.; Daly, Jennifer S.] Univ Massachusetts, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01605 USA. [Wickes, Brian L.] Univ Texas Hlth Sci Ctr, Dept Microbiol & Immunol, San Antonio, TX USA. [Sutton, Deanna A.; Thompson, Elizabeth H.] Univ Texas Hlth Sci Ctr, Dept Pathol, San Antonio, TX USA. [Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. RP Cariello, PF (reprint author), Univ Massachusetts, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01605 USA. EM paloma.cariello@umassmemorial.org NR 12 TC 3 Z9 3 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 2013 VL 51 IS 2 BP 692 EP 695 DI 10.1128/JCM.02674-12 PG 4 WC Microbiology SC Microbiology GA 078OH UT WOS:000314108000057 PM 23196359 ER PT J AU Morris, JB Antonious, GF AF Morris, J. Bradley Antonious, George F. TI Glucose, stem dry weight variation, principal component and cluster analysis for some agronomic traits among 16 regenerated Crotalaria juncea accessions for potential cellulosic ethanol SO JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES LA English DT Article DE Crotalaria juncea; principal component; cluster analysis; agronomic; cellulose; ethanol ID SUNN HEMP; GROWTH AB The objectives of this research were to identify candidate sunn hemp accessions having high concentrations of cellulose for use as parents in breeding for cellulose and to determine variability for glucose content and some important agronomic traits among sunn hemp accessions. Since sunn hemp is an under-utilized species, glucose content and agronomic trait variation is essential for the identification of superior sunn hemp accessions for use as potential ethanol for biofuel. Sixteen sunn hemp accessions including the following plant introductions (expressed as glucose concentration) and stem dry weights were studied. Sixteen sunn hemp accessions including the following plant introductions (expressed as glucose concentration) and stem dry weights were studied. In addition, to verify variability, these traits plus morphological, phenological, and seed reproductive traits were analyzed using multivariate and cluster analysis. The accessions, PI 250487, PI 337080, and PI 219717 produced the highest glucose concentrations (859, 809, and 770mg g1 stem dry weight, respectively), however PI 468956 produced the highest stem dry weight (258g). Branching significantly correlated with foliage (r2=0.67**) and relative maturity (r2=0.60*), while maturity had a significantly negative correlation with seed number (r2=0.67**) and plant width (r2=0.53*) as well. Seed number significantly correlated with plant width (r2=0.57*). Average linkage cluster analysis grouped the 16 sunn hemp accessions into well-defined phenotypes with four distinct seed-producing groups and one outlier. Based on multivariate and cluster analysis, sufficient variation among these16 sunn hemp accessions exists to support the development of cellulosic ethanol producing cultivars with improved architecture, early maturity, seed yield, glucose concentrations, and stem dry weights. C1 [Morris, J. Bradley] ARS, Plant Genet Resources Conservat Unit, USDA, Griffin, GA USA. [Antonious, George F.] Kentucky State Univ, Coll Agr Food Sci & Sustainable Syst, Div Environm Studies, Frankfort, KY USA. RP Morris, JB (reprint author), ARS, Plant Genet Resources Conservat Unit, USDA, Griffin, GA USA. EM Brad.Morris@ars.usda.gov NR 13 TC 1 Z9 1 U1 0 U2 12 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0360-1234 J9 J ENVIRON SCI HEAL B JI J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes PD FEB 1 PY 2013 VL 48 IS 3 BP 214 EP 218 DI 10.1080/03601234.2013.730333 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA 081UF UT WOS:000314347200008 PM 23356343 ER PT J AU Harrison, RL AF Harrison, Robert L. TI Concentration- and time-response characteristics of plaque isolates of Agrotis ipsilon multiple nucleopolyhedrovirus derived from a field isolate SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Baculovirus; Nucleopolyhedrovirus; Agrotis ipsilon; Genotype; Pathogenicity; Virulence ID HELICOVERPA-ARMIGERA NUCLEOPOLYHEDROVIRUS; NUCLEAR POLYHEDROSIS-VIRUS; BLACK-CUTWORM; EGT GENE; DELETION; GENOTYPES; INSECT; POPULATION; HOST; LEPIDOPTERA AB Plaque isolates derived from the Illinois field isolate of Agrotis ipsilon multiple nucleopolyhedrovirus are distinguished by the presence or absence of a small deletion in the baculovirus egt (ecdysteroid UDP-glucosyltransferase) coding sequence. In concentration-response and time-response bioassays with both plaque and field isolates, plaque isolates with a mutated egt gene were less pathogenic against A. ipsilon larvae than other isolates, but killed larvae faster. Mixed infections with isolates representing the two different egt genotypes caused the same level of mortality as the field isolate and a plaque isolate with a wild-type egt gene. Published by Elsevier Inc. C1 ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, USDA, Beltsville, MD 20705 USA. RP Harrison, RL (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, USDA, 011A,Room 214,BARC-W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Robert.L.Harrison@ars.usda.gov RI Harrison, Robert/I-4707-2014; OI Harrison, Robert/0000-0002-8348-3874 NR 22 TC 4 Z9 4 U1 0 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD FEB PY 2013 VL 112 IS 2 BP 159 EP 161 DI 10.1016/j.jip.2012.11.010 PG 3 WC Zoology SC Zoology GA 082GT UT WOS:000314381000010 PM 23220242 ER PT J AU Lehman, RM Rosentrater, KA AF Lehman, R. Michael Rosentrater, Kurt A. TI Aerobic stability of distillers wet grains as influenced by temperature SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE aerobic stability; biofuel; distillers wet grains; ethanol production; storage ID ETHANOL; WHEAT AB BACKGROUND: The storability of distillers wet grains (DWG) influences the economic, energetic, and carbon balances of fuel ethanol production, yet there are limited published data on the deterioration of DWG following its production. We used biogenic CO2 production to assess the aerobic stability of DWG incubated at three temperatures (12 degrees C, 22 degrees C, 32 degrees C) and compared CO2 production over time to the appearance of mold and changes in DWG color parameters. RESULTS: CO2 production and mold colonization indicate that at temperatures near 12 degrees C, the aerobic stability of DWG was high and that it can be stored for at least a 10-day period. At temperatures close to 22 degrees C, the onset of increased microbial activity and visible mold colonization occurred between 4 and 7 days and both activity and mold ratings were very high by the ninth day in all three experiments. At 32 degrees C, 2 days may be a more appropriate limit for storage. CONCLUSION: Temperature and time interact in a nonlinear fashion that permits the prediction of DWG stability boundaries. The simple visual appearance of mold appears to be a reasonable indicator that correlates well (r = 0.694) with CO2 production, a measure of the aerobic stability of DWG. Published 2012 by John Wiley & Sons, Ltd. C1 [Lehman, R. Michael; Rosentrater, Kurt A.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lehman, RM (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM michael.lehman@ars.usda.gov OI Lehman, Michael/0000-0002-3391-3178; Rosentrater, Kurt/0000-0003-0131-7037 NR 16 TC 0 Z9 0 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD FEB PY 2013 VL 93 IS 3 BP 498 EP 503 DI 10.1002/jsfa.5803 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 079NR UT WOS:000314177900010 PM 22865196 ER PT J AU Kilgore, MA Snyder, SA Block-Torgerson, K Taff, SJ AF Kilgore, Michael A. Snyder, Stephanie A. Block-Torgerson, Kayla Taff, Steven J. TI Challenges in characterizing a parcelized forest landscape: Why metric, scale, threshold, and definitions matter SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Parcelization; Gini coefficient; Entropy; Forest landscape ID NONINDUSTRIAL PRIVATE FORESTLAND; NEW-YORK; POPULATION; FRAGMENTATION; INVESTMENT; MANAGEMENT; WISCONSIN; PATTERNS; ENTROPY; OWNERS AB Several metrics have been cited in the literature as being useful characterizations of forest land parcelization. Yet no agreed-upon standard measure exists which creates difficulties in identifying where parcelization is occurring as well as comparing the magnitude of its occurrence across different studies and geographic regions. We evaluated three existing (average parcel size, Gini coefficient, Shannon Entropy index) and a new metric (adjusted mean parcel size) for their usefulness in characterizing the extent to which a private forested landscape has become parcelized. We applied these measures to 410 forested townships in a contiguous, six-county area of northern Minnesota encompassing nearly 3.64 million hectares. Our analyses show that each metric typically describes a different pattern of parcelization and highlight problems owing to the fact that each metric appears to capture different aspects of ownership patterns within a landscape. We demonstrate the choice of metric, landscape scale, spatial and physical ownership features, and threshold for determining when a landscape is parcelized can greatly influence our conclusions regarding parcelization. Thus, careful consideration must be given to these factors when attempting to analyze a parcelized landscape, and caution is urged in interpreting and comparing parcelization studies where one or more of these factors vary. (C) 2012 Elsevier B.V. All rights reserved. C1 [Kilgore, Michael A.; Block-Torgerson, Kayla] Univ Minnesota, Dept Forest Resources, Minneapolis, MN 55455 USA. [Snyder, Stephanie A.] US Forest Serv, USDA, No Res Stn, Morgantown, WV USA. [Taff, Steven J.] Univ Minnesota, Dept Appl Econ, Minneapolis, MN 55455 USA. RP Kilgore, MA (reprint author), Dept Forest Resources, 301K Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. EM mkilgore@umn.edu; stephaniesnyder@fs.fed.us; block147@umn.edu; sjtaff@umn.edu NR 33 TC 4 Z9 4 U1 3 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD FEB PY 2013 VL 110 BP 36 EP 47 DI 10.1016/j.landurbplan.2012.09.009 PG 12 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 079RJ UT WOS:000314189600005 ER PT J AU Falkenberg, SM Carroll, JA Keisler, DH Sartin, JL Elsasser, TH Buntyn, JO Broadway, PR Schmidt, TB AF Falkenberg, S. M. Carroll, J. A. Keisler, D. H. Sartin, J. L. Elsasser, T. H. Buntyn, J. O. Broadway, P. R. Schmidt, T. B. TI Evaluation of the endocrine response of cattle during the relocation process SO LIVESTOCK SCIENCE LA English DT Article DE Transportation stress; Cortisol; Rectal temperature; Cattle; Growth hormone ID GROWTH-HORMONE; RECTAL TEMPERATURE; IGF-I; TRANSPORTATION; CALVES; STRESS; SUPPRESSION; RECEPTOR; BULLS; ROAD AB To evaluate the endocrine responses associated with the relocation process, 22 Holstein heifers (326.4 +/- 46.8 kg BW) were randomly assigned to control (CON) or relocation (RELOC) treatment groups. On d 0, heifers were weighed and fitted with indwelling rectal temperature (RT) monitoring devices and jugular catheters. On d 1, baseline blood samples were collected from all heifers for 2 h prior to the transportation event, then weighed. Controls were returned to tie stalls and RELOC were loaded into a modified stock trailer (12 individual stanchions) for a 4 h transportation event. Simultaneous blood samples were obtained at 30-min intervals from both groups throughout the 4 h transport event (TE-I). After transport, RELOC were unloaded at an unfamiliar location, weighed, and placed in tie stalls for a 2 h post-transportation period. All heifers were then placed into two separate holding paddocks with access to water and hay for 4 h. After 4 h, hay and water was withdrawn for 20 h. On d 2 RELOC heifers were exposed to a second transport event (TE-II); the timeline and procedures of TE-II were identical to those of TE-I (except for the starting point for RELOC heifers). All serum samples were analyzed for concentrations of cortisol, growth hormone (GH), and insulin-like growth factor-I (IGF-I). A 6% reduction in BW for the RELOC as compared to 2.5% reduction in BW for CON (P < 0.001) was observed during TE-I. Overall BW loss was 2% greater (P < 0.02) for RELOC heifers compared to CON heifers. During TE-I, RELOC heifers had greater RT (P < 0.05) compared to CON heifers. There was treatment x time interaction observed for cortisol (P < 0.003); RELOC had greater cortisol concentrations at multiple time points throughout TE-I and -II. No differences (P > 0.05) in area under the curve (AUC) for cortisol were observed during TE-I. However, AUC for total cortisol during TE-II was greater (P < 0.01) in the RELOC group compared to CON. There were no differences in AUC for GH between treatment groups for TE-I or -II, but a transient decline (P < 0.05) within each group was observed from d 1 to d 2. There were no differences (P > 0.05) in IGF-I concentrations or in AUC between the treatment groups during TE-I and -II or from d 1 to d 2. Results provide evidence that the actual processes surrounding the transportation of cattle, can elicit a stress response, as defined by increased concentrations of cortisol, RI, and BW losses. (C) 2012 Elsevier B.V. All rights reserved. C1 [Falkenberg, S. M.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Carroll, J. A.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Keisler, D. H.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Sartin, J. L.] Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA. [Elsasser, T. H.] ARS, USDA, Beltsville, MD 20705 USA. [Buntyn, J. O.] Mississippi State Univ, Dept Anim & Dairy Sci, Mississippi State, MS 39762 USA. [Broadway, P. R.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79403 USA. [Schmidt, T. B.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. RP Schmidt, TB (reprint author), Univ Nebraska, Dept Anim Sci, A213,POB 830908, Lincoln, NE 68583 USA. EM tschmidt4@unl.edu RI Keisler, Duane/C-2746-2011 OI Keisler, Duane/0000-0002-8792-7030 NR 14 TC 3 Z9 3 U1 0 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 J9 LIVEST SCI JI Livest. Sci. PD FEB PY 2013 VL 151 IS 2-3 BP 203 EP 212 DI 10.1016/j.livsci.2012.09.009 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 082XX UT WOS:000314428400014 ER PT J AU Sheppard, SK Didelot, X Jolley, KA Darling, AE Pascoe, B Meric, G Kelly, DJ Cody, A Colles, FM Strachan, NJC Ogden, ID Forbes, K French, NP Carter, P Miller, WG Mccarthy, ND Owen, R Litrup, E Egholm, M Affourtit, JP Bentley, SD Parkhill, J Maiden, MCJ Falush, D AF Sheppard, Samuel K. Didelot, Xavier Jolley, Keith A. Darling, Aaron E. Pascoe, Ben Meric, Guillaume Kelly, David J. Cody, Alison Colles, Frances M. Strachan, Norval J. C. Ogden, Iain D. Forbes, Ken French, Nigel P. Carter, Philip Miller, William G. Mccarthy, Noel D. Owen, Robert Litrup, Eva Egholm, Michael Affourtit, Jason P. Bentley, Stephen D. Parkhill, Julian Maiden, Martin C. J. Falush, Daniel TI Progressive genome-wide introgression in agricultural Campylobacter coli SO MOLECULAR ECOLOGY LA English DT Article DE adaptation; Campylobacter; epistasis; genomics; introgression ID EVOLUTIONARY RATE; GENE-TRANSFER; JEJUNI; SEQUENCE; SPECIATION; RECOMBINATION; CONVERGENCE; DIVERSITY; ALIGNMENT; BACTERIA AB Hybridization between distantly related organisms can facilitate rapid adaptation to novel environments, but is potentially constrained by epistatic fitness interactions among cell components. The zoonotic pathogens Campylobacter coli and C. jejuni differ from each other by around 15% at the nucleotide level, corresponding to an average of nearly 40 amino acids per protein-coding gene. Using whole genome sequencing, we show that a single C. coli lineage, which has successfully colonized an agricultural niche, has been progressively accumulating C. jejuni DNA. Members of this lineage belong to two groups, the ST-828 and ST-1150 clonal complexes. The ST-1150 complex is less frequently isolated and has undergone a substantially greater amount of introgression leading to replacement of up to 23% of the C. coli core genome as well as import of novel DNA. By contrast, the more commonly isolated ST-828 complex bacteria have 1011% introgressed DNA, and C. jejuni and nonagricultural C. coli lineages each have <2%. Thus, the C. coli that colonize agriculture, and consequently cause most human disease, have hybrid origin, but this cross-species exchange has so far not had a substantial impact on the gene pools of either C. jejuni or nonagricultural C. coli. These findings also indicate remarkable interchangeability of basic cellular machinery after a prolonged period of independent evolution. C1 [Sheppard, Samuel K.; Jolley, Keith A.; Cody, Alison; Colles, Frances M.; Mccarthy, Noel D.; Maiden, Martin C. J.] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. [Sheppard, Samuel K.; Pascoe, Ben; Meric, Guillaume] Swansea Univ, Inst Life Sci, Coll Med, Swansea SA2 8PP, W Glam, Wales. [Didelot, Xavier] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London W2 1PG, England. [Kelly, David J.] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England. [Strachan, Norval J. C.; Ogden, Iain D.] Univ Aberdeen, Sch Med & Dent, Aberdeen AB25 2ZD, Scotland. [Forbes, Ken] Univ Aberdeen, Sch Biol Sci, Aberdeen AB25 2ZD, Scotland. [French, Nigel P.] Massey Univ, Hopkirk Res Inst, Inst Vet Anim & Biomed Sci, Palmerston North, New Zealand. [Carter, Philip] Inst Environm Sci & Res, Wellington, New Zealand. [Miller, William G.] USDA, Albany, CA 94710 USA. [Owen, Robert] Hlth Protect Agcy, Ctr Infect, London NW9 5EQ, England. [Litrup, Eva] Statens Serum Inst, DK-2300 Copenhagen S, Denmark. [Egholm, Michael] Pall Corp, Port Washington, NY 11050 USA. [Affourtit, Jason P.] Ion Torrent, Guilford, CT 06437 USA. [Bentley, Stephen D.; Parkhill, Julian] Wellcome Turst Sanger Inst, Cambridge CB10 1SA, England. RP Sheppard, SK (reprint author), Univ Oxford, Dept Zool, Tinbergen Bldg,S Parks Rd, Oxford OX1 3PS, England. EM s.k.sheppard@swansea.ac.uk RI Pascoe, Ben/C-2253-2009; Didelot, Xavier/H-1608-2013; Parkhill, Julian/G-4703-2011; French, Nigel/F-5725-2011; OI Darling, Aaron/0000-0003-2397-7925; Maiden, Martin/0000-0001-6321-5138; Pascoe, Ben/0000-0001-6376-5121; Didelot, Xavier/0000-0003-1885-500X; Parkhill, Julian/0000-0002-7069-5958; Meric, Guillaume/0000-0001-6288-9958; Sheppard, Samuel/0000-0001-6901-3203; Jolley, Keith/0000-0002-0751-0287 FU Wellcome Trust; BBSRC FX This work was carried out with support from the Wellcome Trust and the BBSRC. Dr Sheppard is a Wellcome Trust Fellow. NR 58 TC 32 Z9 32 U1 0 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD FEB PY 2013 VL 22 IS 4 BP 1051 EP 1064 DI 10.1111/mec.12162 PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 080DH UT WOS:000314220900012 PM 23279096 ER PT J AU You, MS Yue, Z He, WY Yang, XH Yang, G Xie, M Zhan, DL Baxter, SW Vasseur, L Gurr, GM Douglas, CJ Bai, JL Wang, P Cui, K Huang, SG Li, XC Zhou, Q Wu, ZY Chen, QL Liu, CH Wang, B Li, XJ Xu, XF Lu, CX Hu, M Davey, JW Smith, SM Chen, MS Xia, XF Tang, WQ Ke, FS Zheng, DD Hu, YL Song, FQ You, YC Ma, XL Peng, L Zheng, YK Liang, Y Chen, YQ Yu, LY Zhang, YN Liu, YY Li, GQ Fang, L Li, JX Zhou, X Luo, YD Gou, CY Wang, JY Wang, J Yang, HM Wang, J AF You, Minsheng Yue, Zhen He, Weiyi Yang, Xinhua Yang, Guang Xie, Miao Zhan, Dongliang Baxter, Simon W. Vasseur, Liette Gurr, Geoff M. Douglas, Carl J. Bai, Jianlin Wang, Ping Cui, Kai Huang, Shiguo Li, Xianchun Zhou, Qing Wu, Zhangyan Chen, Qilin Liu, Chunhui Wang, Bo Li, Xiaojing Xu, Xiufeng Lu, Changxin Hu, Min Davey, John W. Smith, Sandy M. Chen, Mingshun Xia, Xiaofeng Tang, Weiqi Ke, Fushi Zheng, Dandan Hu, Yulan Song, Fengqin You, Yanchun Ma, Xiaoli Peng, Lu Zheng, Yunkai Liang, Yong Chen, Yaqiong Yu, Liying Zhang, Younan Liu, Yuanyuan Li, Guoqing Fang, Lin Li, Jingxiang Zhou, Xin Luo, Yadan Gou, Caiyun Wang, Junyi Wang, Jian Yang, Huanming Wang, Jun TI A heterozygous moth genome provides insights into herbivory and detoxification SO NATURE GENETICS LA English DT Article ID DIAMONDBACK MOTH; PLUTELLA-XYLOSTELLA; RNA-SEQ; LTR RETROTRANSPOSONS; BOMBYX-MORI; GENE; SEQUENCE; RESISTANCE; LEPIDOPTERA; DATABASE AB How an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants(1), but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood(2). We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management. C1 [You, Minsheng; He, Weiyi; Yang, Guang; Xie, Miao; Vasseur, Liette; Gurr, Geoff M.; Douglas, Carl J.; Bai, Jianlin; Cui, Kai; Huang, Shiguo; Liu, Chunhui; Li, Xiaojing; Xu, Xiufeng; Smith, Sandy M.; Xia, Xiaofeng; Tang, Weiqi; Ke, Fushi; Zheng, Dandan; Hu, Yulan; Song, Fengqin; You, Yanchun; Ma, Xiaoli; Peng, Lu; Zheng, Yunkai; Liang, Yong; Chen, Yaqiong; Yu, Liying; Zhang, Younan] Fujian Agr & Forestry Univ, Inst Appl Ecol, Fuzhou, Peoples R China. [You, Minsheng; He, Weiyi; Yang, Guang; Xie, Miao; Vasseur, Liette; Gurr, Geoff M.; Douglas, Carl J.; Bai, Jianlin; Cui, Kai; Huang, Shiguo; Liu, Chunhui; Li, Xiaojing; Xu, Xiufeng; Smith, Sandy M.; Xia, Xiaofeng; Tang, Weiqi; Ke, Fushi; Zheng, Dandan; Hu, Yulan; Song, Fengqin; You, Yanchun; Ma, Xiaoli; Peng, Lu; Zheng, Yunkai; Liang, Yong; Chen, Yaqiong; Yu, Liying; Zhang, Younan] Minist Agr, Key Lab Integrated Pest Management Fujian Taiwan, Fuzhou, Peoples R China. [Yue, Zhen; Yang, Xinhua; Zhan, Dongliang; Zhou, Qing; Wu, Zhangyan; Chen, Qilin; Wang, Bo; Lu, Changxin; Hu, Min; Li, Guoqing; Fang, Lin; Li, Jingxiang; Zhou, Xin; Luo, Yadan; Gou, Caiyun; Wang, Junyi; Wang, Jian; Yang, Huanming; Wang, Jun] BGI Shenzhen, Shenzhen, Peoples R China. [Baxter, Simon W.] Univ Cambridge, Dept Zool, Cambridge, England. [Baxter, Simon W.] Univ Adelaide, Sch Mol & Biomed Sci, Adelaide, SA, Australia. [Vasseur, Liette] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada. [Gurr, Geoff M.] Charles Sturt Univ, EH Graham Ctr, Orange, NSW, Australia. [Douglas, Carl J.; Liu, Yuanyuan] Univ British Columbia, Dept Bot, Vancouver, BC, Canada. [Wang, Ping] Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. [Li, Xianchun] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Davey, John W.] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh, Midlothian, Scotland. [Smith, Sandy M.] Univ Toronto, Fac Forestry, Toronto, ON, Canada. [Chen, Mingshun] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Chen, Mingshun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Wang, Jun] Univ Copenhagen, Dept Biol, Copenhagen, Denmark. RP You, MS (reprint author), Fujian Agr & Forestry Univ, Inst Appl Ecol, Fuzhou, Peoples R China. EM msyou@iae.fjau.edu.cn; yxg@iae.fjau.edu.cn; wangj@genomics.cn RI Davey, John/B-5075-2013; Zhou, Xin/D-4025-2009; Wang, Jun/A-7261-2013; Wang, Jun/C-8434-2016; Wang, Jun/B-9503-2016 OI Zhou, Xin/0000-0002-1407-7952; Wang, Jun/0000-0002-1422-3331; Wang, Jun/0000-0002-8540-8931; Gurr, Geoff/0000-0001-5008-7966; Davey, John/0000-0002-1017-9775; Wang, Jun/0000-0002-2113-5874 FU Educational Department of Fujian Province [JB09315]; National Natural Science Foundation of China [31230061] FX This work was supported through a special project of Research on Diamondback Moth Genomics (grant JB09315) to M.Y. and a Minjiang Scholar Program to L.V., G.M.G., C.J.D. and S.M.S. by the Educational Department of Fujian Province and through a key project (grant 31230061) to M.Y. from the National Natural Science Foundation of China. Insect rearing and sampling, as well as some of the DNA extractions, were conducted at the Fujian Provincial Key Laboratory of Biodiversity and Eco-safety and the Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, the Ministry of Agriculture, China. We are grateful to AD. Briscoe (University of California Irvine) for her help in organizing and for providing ORs, OBPs and CSPs from Danaus plexippus and Heliconius melpomene and to G.L. Lovei (Aarhus University) for his comments and suggestions on the manuscript. We appreciate J. Liao and M. Zou (Fujian Agriculture and Forestry University) for providing the Bt-treated P. xylostella larvae used for quantitative gene expression analysis. We thank H. Wang, J. Luo, Y. Hong, S. Pan, L. Yang, Y. Weng, Y. Hong and Y. Liu for their technical assistance in rearing insects and preparing samples. NR 85 TC 144 Z9 176 U1 13 U2 170 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD FEB PY 2013 VL 45 IS 2 BP 220 EP 225 DI 10.1038/ng.2524 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA 081PB UT WOS:000314333000020 PM 23313953 ER PT J AU Weldon, PJ Cardoza, YJ Vander Meer, RK Hoffmann, WC Daly, JW Spande, TF AF Weldon, Paul J. Cardoza, Yasmin J. Vander Meer, Robert K. Hoffmann, W. Clint Daly, John W. Spande, Thomas F. TI Contact toxicities of anuran skin alkaloids against the fire ant (Solenopsis invicta) SO NATURWISSENSCHAFTEN LA English DT Article DE Alkaloid; Allomone; Ant; Defense; Frog; Toxicity ID DENDROBATID POISON FROGS; PUMILIOTOXIN 251D; DECAHYDROQUINOLINES; MELANOPHRYNISCUS; QUINOLIZIDINES; PYRROLIZIDINES; INDOLIZIDINES; BUFONIDAE; DEFENSE; COMMON AB Nearly 500 alkaloids, representing over 20 structural classes, have been identified from the skin of neotropical poison frogs (Dendrobatidae). These cutaneous compounds, which are derived from arthropod prey of the frogs, generally are believed to deter predators. We tested the red imported fire ant (Solenopsis invicta) for toxicosis following contact with 20 alkaloids (12 structural classes) identified from dendrobatids or other anurans. Individual ants forced to contact the dried residues of 13 compounds exhibited convulsions and/or reduced ambulation. We estimated the cutaneous concentrations of several compounds based on their reported recoveries from skin extracts of free-ranging frogs and our measurements of the skin surface areas of museum specimens. Pumiliotoxin 251D exhibited contact toxicity below its estimated cutaneous concentration in the Ecuadorian frog, Epipedobates anthonyi, an observation consistent with the hypothesized role of this compound in anuran chemical defense. Our results and those of a previous study of mosquitoes indicate that some anuran skin compounds function defensively as contact toxins against arthropods, permeating their exoskeleton. C1 [Weldon, Paul J.] Natl Zool Pk, Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. [Cardoza, Yasmin J.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Vander Meer, Robert K.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Hoffmann, W. Clint] ARS, So Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [Daly, John W.; Spande, Thomas F.] NIDDKD, Dept Hlth & Human Serv, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. RP Vander Meer, RK (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. EM bob.vandermeer@ars.usda.gov NR 41 TC 4 Z9 4 U1 1 U2 56 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0028-1042 J9 NATURWISSENSCHAFTEN JI Naturwissenschaften PD FEB PY 2013 VL 100 IS 2 BP 185 EP 192 DI 10.1007/s00114-013-1010-0 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 080WT UT WOS:000314275500008 PM 23340579 ER PT J AU Hernandez, SM Galbreath, B Riddle, DF Moore, AP Palamar, MB Levy, MG DePerno, CS Correa, MT Yabsley, MJ AF Hernandez, Sonia M. Galbreath, Brianna Riddle, Dennis F. Moore, Andrew P. Palamar, Maria B. Levy, Michael G. DePerno, Christopher S. Correa, Maria T. Yabsley, Michael J. TI Baylisascaris procyonis in raccoons (Procyon lotor) from North Carolina and current status of the parasite in the USA SO PARASITOLOGY RESEARCH LA English DT Article ID SOUTHEASTERN UNITED-STATES; HELMINTH-PARASITES; SOUTH-CAROLINA; MARMOTA-MONAX; GEORGIA; RABIES; FLORIDA; ISLAND; PREVALENCE; ROUNDWORMS AB Baylisascaris procyonis is an intestinal nematode of raccoons (Procyon lotor) that can cause fatal larva migrans in numerous species of birds and mammals, including humans. Historically, this parasite has been rare in the southeastern USA but recently has been reported in eastern Tennessee and isolated parts of Georgia and Florida. The objective of the current study was to investigate the distribution and prevalence of B. procyonis in raccoons from North Carolina. In western North Carolina, in counties bordering Tennessee, B. procyonis was detected in nine of 74 (12 %) raccoons sampled in 2010-2011. In general, worm burdens (average 20 worms) were low, but one raccoon had 122 adult worms. No difference was noted in prevalence by year or age, but significantly more males were infected compared with females. Sequences of the internal transcribed spacer 2 region from three samples were identical to B. procyonis. In central North Carolina (Guilford County), all 34 raccoons and 49 fecal samples tested were negative. Collation of data from previous studies conducted in the Southeast indicates that B. procyonis has been reported from numerous counties, but surveillance has been patchy and many negative results are > 30 years old. These results indicate that B. procyonis is established in North Carolina and given the zoonotic and wildlife health implications of this parasite, additional surveillance in North Carolina and other southeastern states is warranted. C1 [Hernandez, Sonia M.; Galbreath, Brianna; Yabsley, Michael J.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Hernandez, Sonia M.; Yabsley, Michael J.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, SE Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Riddle, Dennis F.; Moore, Andrew P.] US Anim & Plant Hlth Inspect Serv, Wildlife Serv, USDA, Raleigh, NC USA. [Palamar, Maria B.; DePerno, Christopher S.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Palamar, Maria B.; Levy, Michael G.; Correa, Maria T.] N Carolina State Univ, Coll Vet Med Populat Hlth & Pathobiol, Raleigh, NC 27695 USA. RP Hernandez, SM (reprint author), Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. EM shernz@uga.edu FU SCWDS FX The authors thank numerous personnel from the USDA for field assistance and from the SCWDS for necropsy assistance. We also thank B. Shock for assistance with the figure. Additional support was provided by the Federal Aid to Wildlife Restoration Act (50 Stat. 917) and through SCWDS sponsorship from fish and wildlife agencies of member states. NR 44 TC 12 Z9 13 U1 1 U2 39 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 J9 PARASITOL RES JI Parasitol. Res. PD FEB PY 2013 VL 112 IS 2 BP 693 EP 698 DI 10.1007/s00436-012-3186-1 PG 6 WC Parasitology SC Parasitology GA 077YF UT WOS:000314064600029 PM 23180125 ER PT J AU Landolt, PJ Toth, M Meagher, RL Szarukan, I AF Landolt, Peter J. Toth, Miklos Meagher, Robert L. Szarukan, Istvan TI Interaction of acetic acid and phenylacetaldehyde as attractants for trapping pest species of moths (Lepidoptera: Noctuidae) SO PEST MANAGEMENT SCIENCE LA English DT Article DE acetic acid; attractant; lure; moth; phenylacetaldehyde; insect trap ID CABBAGE-LOOPER MOTHS; XESTIA-C-NIGRUM; ALFALFA LOOPER; VOLATILE CONSTITUENTS; MAMESTRA-CONFIGURATA; CHEMICAL ATTRACTANTS; FEEDING ATTRACTANTS; LACANOBIA-SUBJUNCTA; FLORAL COMPOUNDS; SOYBEAN LOOPER AB BACKGROUND: Phenylacetaldehyde is a flower volatile and attractant for many nectar-seeking moths. Acetic acid is a microbial fermentation product that is present in insect sweet baits. It is weakly attractive to some moths and other insects, but can be additive or synergistic with other compounds to make more powerful insect lures. RESULTS: Acetic acid and phenylacetaldehyde presented together in traps made a stronger lure than either chemical alone for moths of the alfalfa looper Autographa californica (Speyer) and the armyworm Spodoptera albula (Walker). However, this combination of chemicals reduced captures of the cabbage looper moth Trichoplusia ni (Hubner), the silver Y moth Autographa gamma (L.), MacDunnoughia confusa (Stephens) and the soybean looper moth Chrysodeixis includens (Walker) by comparison with phenylacetaldehyde alone. CONCLUSION: These results indicate both positive and negative interactions of acetic acid, a sugar fermentation odor cue, and phenylacetaldehyde, a floral scent cue, in eliciting orientation responses of moths. This research provides a new two-component lure for the alfalfa looper A. californica and for the armyworm S. albula for potential use in pest management. Copyright (C) 2012 Society of Chemical Industry C1 [Landolt, Peter J.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Toth, Miklos] Hungarian Acad Sci, Inst Plant Protect, Agr Res Ctr, Budapest, Hungary. [Meagher, Robert L.] ARS, USDA, CMAVE, Gainesville, FL USA. [Szarukan, Istvan] Univ Debrecen, Dept Plant Protect, Fac Agr Sci, H-4012 Debrecen, Hungary. RP Landolt, PJ (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov FU Washington State Potato Commission; OTKA of Hungarian Academy of Sciences [K 81494] FX The authors thank Anita Bagarus, Jewel Brumley, Daryl Green, Gracie Galindo, James Hansen, Sandor Koczor and Amy Rowley for technical assistance. This work was supported in part by funding from the Washington State Potato Commission, and by OTKA grant K 81494 of the Hungarian Academy of Sciences. Helpful suggestions to improve the manuscript were made by Joseph Munyaneza and Richard Zack. NR 36 TC 6 Z9 7 U1 2 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2013 VL 69 IS 2 BP 245 EP 249 DI 10.1002/ps.3381 PG 5 WC Agronomy; Entomology SC Agriculture; Entomology GA 076UN UT WOS:000313983900016 PM 22887357 ER PT J AU Christopher, MD Liu, SY Hall, MD Marshall, DS Fountain, MO Johnson, JW Milus, EA Garland-Campbell, KA Chen, XM Griffey, CA AF Christopher, Mark D. Liu, Shuyu Hall, Marla D. Marshall, David S. Fountain, Myron O. Johnson, Jerry W. Milus, Eugene A. Garland-Campbell, Kimberly A. Chen, Xianming Griffey, Carl A. TI Identification and mapping of adult-plant stripe rust resistance in soft red winter wheat cultivar 'USG 3555' SO PLANT BREEDING LA English DT Article DE Puccinia striiformis; yellow rust; quantitative trait loci; marker-assisted selection ID F-SP TRITICI; QUANTITATIVE TRAIT LOCI; PUCCINIA-STRIIFORMIS; LEAF RUST; UNITED-STATES; BREAD WHEAT; SPRING WHEAT; YELLOW-RUST; ISOGENIC LINES; GENES AB Little is known about the extent or diversity of resistance in soft red winter wheat (Triticum aestivum L.) to stripe rust, caused by the fungal pathogen Puccinia striiformis f.sp. tritici. The soft red winter (SRW) wheat cultivar USG 3555 has effective adult-plant resistance to stripe rust, which was characterized in a population derived from USG 3555/Neuse. The mapping population consisted of 99 recombinant inbred lines, which were evaluated for stripe rust infection type (IT) and severity to race PST-100 in field trials in North Carolina in 2010 and 2011. Genome-wide molecular-marker screenings with 119 simple sequence repeats and 560 Diversity Arrays Technology (DArT) markers were employed to identify quantitative trait loci (QTL) for stripe rust resistance. QTL on chromosomes 1AS, 4BL and 7D of USG 3555 explained 12.8, 73.0 and 13.6% of the variation in stripe rust IT, and 13.5, 72.3 and 10.5% of the variation in stripe rust severity, respectively. Use of these and additional diagnostic markers for these QTL will facilitate the introgression of this source of stripe rust resistance into SRW wheat lines via marker-assisted selection. C1 [Christopher, Mark D.; Hall, Marla D.; Griffey, Carl A.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Liu, Shuyu] TAMU, Texas AgriLife Res, Amarillo, TX 79106 USA. [Marshall, David S.; Fountain, Myron O.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Johnson, Jerry W.] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Milus, Eugene A.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Garland-Campbell, Kimberly A.; Chen, Xianming] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Christopher, MD (reprint author), Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. EM Christm@vt.edu FU Virginia Small Grains Board; Virginia Agricultural Council; U.S. Department of Agriculture [58-6 645-7-255] FX This study was supported with funding provided by the Virginia Small Grains Board and the Virginia Agricultural Council. This material is based on work supported by the U.S. Department of Agriculture, under Agreement No. 58-6 645-7-255. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 45 TC 7 Z9 7 U1 1 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD FEB PY 2013 VL 132 IS 1 BP 53 EP 60 DI 10.1111/pbr.12015 PG 8 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 075NH UT WOS:000313893100007 ER EF