FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Stancic, IB Bosnjak, DV Radovic, IB Stancic, BL Harvey, RB Anderson, RC AF Stancic, Ivan B. Bosnjak, Darko V. Radovic, Ivan B. Stancic, Blagoje L. Harvey, Roger B. Anderson, Robin C. TI Ovarian reaction and estrus manifestation in delayed puberty gilts after treatment with equine chorionic gonadotropin SO REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY LA English DT Article DE Delayed puberty; Ovarian reaction; Estrus; eCG; Gilts ID LANOSTEROL 14-ALPHA-DEMETHYLASE EXPRESSION; REPRODUCTIVE FAILURE; OOCYTE MATURATION; PERFORMANCE; GROWTH AB Background: Prolonged pre-insemination anestrus (i.e. delayed puberty) is a major contributing factor for culling up to 30% of the replacement gilts at large breeding farm units in Vojvodina. It is imperative to determine if these gilts are acyclic (prepubertal) or cyclic, but just fail to exhibit behavioural estrus. Recent investigations demonstrate that treatment with equine chorionic gonadotropin (eCG) can increase the diestrous phase duration in sexually mature gilts. Based on these finding, the aim of the present studies was to determine the reproductive status of delayed puberty gilts following injection with eCG. Methods: Two experiments were conducted on a swine breeding farm in Vojvodina. In Exp. 1, 20 prepubertal (acyclic) gilts, and 120 sexually mature (cyclic) gilts were injected with a single injection of 400 IU eCG + 200 IU human chorionic gonadotropin (hCG) or with 1000 IU eCG (cyclic gilts), at d5, d11 or d17 after spontaneous estrus detection, to determine their ovarian reaction and induced estrus manifestation. In Exp. 2, sixty delayed puberty gilts (estrus not detected until 8 month of age, av. 258 days) were culled from breeding herd and slaughtered to determine their reproductive status based on ovarian anatomical features. The second group of gilts (n = 60) was treated with a single 1000 IU eCG injection to determine their reproductive status, based on the interval between eCG injection to estrus detection and duration. The data were analyzed by descriptive statistics, t-test, analysis of variance and Duncan's test in the software package Statistics 10th. Results: Ovulations were induced in 90% of acyclic (sexually immature) and, on average, 93.3% of cyclic (sexually mature) gilts after the eCG injection. On average, 4 days after the eCG injection, estrus was detected in 85% of the treated acyclic (sexually immature) gilts and in 95% (19/20) of the cyclic (sexually mature) gilts, treated with eCG on day 17 after spontaneous estrus detection. The interval from eCG to induced estrus detection was prolonged (av. 25 days) in 95% (19/20) of the sexually mature gilts treated with eCG on day 5 and in 90% (18/20) of gilts treated on day 11 after spontaneous estrus detection (Exp. 1). Forty anestrous gilts reached cyclic pubertal ovarian activity. Estrus manifestation was detected in 56 gilts (93.3% of the total 60 treated prolonged anestrous gilts, av. 259 days of age), after a single 1000 IU eCG injection. Thirty-four gilts (60.7% of the total gilts in estrus) with prolonged eCG to estrus interval (av. 24.7 days) were considered spontaneously cyclic (sexually mature), but behaviourally anestrous before treatment. The remaining 22 (39.3% of the total gilts in estrus) were considered truly sexually immature (acyclic) before the treatment or were eCG injected in the late luteal or proestrous phase of spontaneous estrous cycle (Exp. 2). Conclusions: In 66.7% of the delayed puberty gilts, pre-ovulatory follicles (PoF), corpora hemorrhagica (CH), corpora lutea (CL), or corpora albicantia (CA) were found on the ovaries upon post mortem examination. These gilts were considered as sexually mature before slaughtering. In 60.7% of the delayed puberty gilts, behavioural estrus was detected an average of 24.7 days following eCG injections. These gilts were considered as eCG treated during the luteal phase (diestrus) of the spontaneous estrus cycle. Both findings suggest that delayed puberty gilts actually reached cyclic pubertal ovarian activity (sexual maturity) before culling from the breeding herd. C1 [Stancic, Ivan B.; Bosnjak, Darko V.; Radovic, Ivan B.; Stancic, Blagoje L.] Univ Novi Sad, Fac Agr, Novi Sad 21000, Serbia. [Harvey, Roger B.; Anderson, Robin C.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX 77845 USA. RP Stancic, IB (reprint author), Univ Novi Sad, Fac Agr, Trg D Obradovica 8, Novi Sad 21000, Serbia. EM dr.ivan.stancic@gmail.com NR 31 TC 0 Z9 1 U1 0 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1477-7827 J9 REPROD BIOL ENDOCRIN JI Reprod. Biol. Endocrinol. PD AUG 22 PY 2012 VL 10 AR 61 DI 10.1186/1477-7827-10-61 PG 5 WC Endocrinology & Metabolism; Reproductive Biology SC Endocrinology & Metabolism; Reproductive Biology GA 007TP UT WOS:000308911500001 PM 22913331 ER PT J AU Grum, DS Cook, D Gardner, DR Roper, JM Pfister, JA Ralphs, MH AF Grum, Daniel S. Cook, Daniel Gardner, Dale R. Roper, Jessie M. Pfister, James A. Ralphs, Michael H. TI Influence of Seed Endophyte Amounts on Swainsonine Concentrations in Astragalus and Oxytropis Locoweeds SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE locoweed; swainsonine; endophyte; Undifilum; qPCR; inoculation ID LYSOSOMAL STORAGE DISEASE; EMBELLISIA-OXYTROPIS; FUNGAL ENDOPHYTES; TALL FESCUE; IDENTIFICATION; TRANSMISSION; UNDIFILUM; ALKALOIDS; GOATS; SPP. AB Locoism is a toxic syndrome of livestock caused by the ingestion of a subset of legumes known as locoweeds endemic to arid and semiarid regions of the western United States. Locoweeds contain the toxic alkaloid swainsonine, which is produced by the endophytic fungi Undifilum species. Two chemotypes of plants can coexist within toxic populations of locoweeds: chemotype 1 plants are defined as individuals containing swainsonine concentrations greater than 0.01% and quantitatively greater amounts of Undifilum, while chemotype 2 plants are defined as individuals containing less than 0.01% swainsonine and quantitatively smaller amounts of Undifilum. To elucidate the mechanisms that govern chemotypes, the amount of Undifilum in seeds/embryos was manipulated, thus altering subsequent swainsonine concentrations in three locoweed species: Astragalus mollissimus, Astragalus lentiginosus, and Oxytropis sericea. Chemotype 1 seeds that were fungicide-treated or had the seed coat removed resulted in plants with swainsonine concentrations comparable to those in chemotype 2 plants. Conversely, embryos from seeds of chemotypes 1 and 2 that were inoculated with the endophyte resulted in plants with swainsonine concentrations comparable to those of chemotype 1 plants. This reproducible interconversion between the two swainsonine chemotypes suggests that the quantity of endophyte present in the seed at the time of germination is a key determinant of the eventual chemotype. Additionally, this is the first report of the inoculation of locoweeds with the endophyte Undifilum species. C1 [Grum, Daniel S.; Cook, Daniel; Gardner, Dale R.; Roper, Jessie M.; Pfister, James A.; Ralphs, Michael H.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. RP Cook, D (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 31 TC 9 Z9 10 U1 1 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 22 PY 2012 VL 60 IS 33 BP 8083 EP 8089 DI 10.1021/jf3024062 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 991KC UT WOS:000307698900002 PM 22844873 ER PT J AU Beck, JJ Higbee, BS Light, DM Gee, WS Merrill, GB Hayashi, JM AF Beck, John J. Higbee, Bradley S. Light, Douglas M. Gee, Wai S. Merrill, Glory B. Hayashi, Jennifer M. TI Hull Split and Damaged Almond Volatiles Attract Male and Female Navel Orangeworm Moths SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE almond; Amyelois transitella; field trapping; navel orangeworm; Prunus dulcis; semiochemical; volatile ID LEPIDOPTERA-PYRALIDAE; SEX-PHEROMONE; AMYELOIS-TRANSITELLA; ACETIC-ACID; IDENTIFICATION; COMMUNICATION; DISRUPTION; PISTACHIOS; BLEND AB A blend of volatiles derived from the emissions of almonds at hull split and mechanically damaged almonds was compared to almond meal, the current monitoring standard for the insect pest navel orangeworm (NOW). Field trapping studies were performed to determine the blend's ability to attract adult NOW. The blend comprised racemic 1-octen-3-ol, ethyl benzoate, methyl salicylate, acetophenone, and racemic (E)-conophthorin. Ethyl acetate was used as a solvent with a blend component concentration of 100 mg/mL. The blend attracted both sexes of NOW when tested in five 2-week intervals spanning the first three flights of NOW in commercial almond orchards in the southern Central Valley of California. The blend demonstrated consistently higher capture rates for female NOW throughout the evaluation period, but unlike almond meal it significantly attracted males. Reported is a survey of the major and minor volatiles emitted from almonds at hull split, the key period of vulnerability to NOW infestation. Also reported is the attractancy of a formulated test blend based on the host plant volatile emissions, electroantennographic screening experiments, and field trapping studies. The results of this test blend highlight progress toward a host-plant-based attractant for NOW, a major insect pest of California tree nuts that presently lacks an adequate monitoring tool. C1 [Beck, John J.; Light, Douglas M.; Gee, Wai S.; Merrill, Glory B.; Hayashi, Jennifer M.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. RP Beck, JJ (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM john.beck@ars.usda.gov FU USDA-ARS CRIS [532S-42000-037-00D]; Paramount Farming Company [CRADA 58-3K95-7-1198]; Almond Board of California [TFCA 58-5325-8-419] FX Research was conducted under USDA-ARS CRIS Project 532S-42000-037-00D, CRADA 58-3K95-7-1198 with Paramount Farming Company, and TFCA 58-5325-8-419 with the Almond Board of California. NR 29 TC 22 Z9 23 U1 0 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 22 PY 2012 VL 60 IS 33 BP 8090 EP 8096 DI 10.1021/jf302658v PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 991KC UT WOS:000307698900003 PM 22849631 ER PT J AU Lawson, LP Vander Meer, RK Shoemaker, D AF Lawson, Lucinda P. Vander Meer, Robert K. Shoemaker, DeWayne TI Male reproductive fitness and queen polyandry are linked to variation in the supergene Gp-9 in the fire ant Solenopsis invicta SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE conflicting selection; facultative polyandry; Solenopsis invicta; supergene ID COLONY GENETIC-STRUCTURE; SEX-RATIO CONFLICT; SOCIAL-ORGANIZATION; KIN SELECTION; EUSOCIAL HYMENOPTERA; MONOGYNE COLONIES; FORMICIDAE; EVOLUTION; INSECTS; STRATEGIES AB Supergenes are clusters of tightly linked loci maintained in specific allelic combinations to facilitate co-segregation of genes governing adaptive phenotypes. In species where strong selection potentially operates at different levels (e.g. eusocial Hymenoptera), positive selection acting within a population to maintain specific allelic combinations in supergenes may have unexpected consequences for some individuals, including the preservation of disadvantageous traits. The nuclear gene Gp-9 in the invasive fire ant Solenopsis invicta is part of a non-recombining, polymorphic supergene region associated with polymorphism in social organization as well as traits affecting physiology, fecundity and behaviour. We show that both male reproductive success and facultative polyandry in queens have a simple genetic basis and are dependent on male Gp-9 genotype. Gp-9(b) males are unable to maintain exclusive reproductive control over their mates such that queens mated to Gp-9(b) males remain highly receptive to remating. Queens mated to multiple Gp-9(B) males are rare. This difference appears to be independent of mating plug production in fertile males of each Gp-9 genotype. However, Gp-9(b) males have significantly lower sperm counts than Gp-9(B) males, which could be a cue to females to seek additional mates. Despite the reduced fitness of Gp-9(b) males, polygyne worker-induced selective mortality of sexuals lacking b-like alleles coupled with the overall success of the polygyne social form act to maintain the Gp-9(b) allele within nature. Our findings highlight how strong worker-induced selection acting to maintain the Gp-9(b) allele in the polygyne social form may simultaneously result in reduced reproductive fitness for individual sexual offspring. C1 [Lawson, Lucinda P.; Vander Meer, Robert K.; Shoemaker, DeWayne] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Lawson, LP (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600-1700 SW 23rd Dr, Gainesville, FL USA. EM lucinda.lawson@ars.usda.gov FU US Department of Agriculture AFRI Award [2009-35302-05301] FX We thank K. Ross, S. Branco, J. Miller, D. Moen, D. Oi and two anonymous reviewers for comments on earlier versions of this manuscript, and E. Carroll for her tireless dedication to completing this project. This study was funded by US Department of Agriculture AFRI Award 2009-35302-05301. NR 49 TC 16 Z9 16 U1 3 U2 38 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD AUG 22 PY 2012 VL 279 IS 1741 BP 3217 EP 3222 DI 10.1098/rspb.2012.0315 PG 6 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA 973DQ UT WOS:000306335300013 PM 22535783 ER PT J AU Zhu, H Xia, R Zhao, BY An, YQ Dardick, CD Callahan, AM Liu, ZR AF Zhu, Hong Xia, Rui Zhao, Bingyu An, Yong-qiang Dardick, Chris D. Callahan, Ann M. Liu, Zongrang TI Unique expression, processing regulation, and regulatory network of peach (Prunus persica) miRNAs SO BMC PLANT BIOLOGY LA English DT Article DE miRNA; Deep sequencing; Prunus persica; Pre-miRNA processing; Trans-acting siRNA; MYB ID PENTATRICOPEPTIDE REPEAT PROTEINS; TRANS-ACTING SIRNAS; SMALL RNAS; ARABIDOPSIS-THALIANA; PLANT MICRORNAS; COMPUTATIONAL IDENTIFICATION; TRANSCRIPTION FACTORS; PRECISE SEQUENCES; BIOGENESIS; TARGETS AB Background: MicroRNAs (miRNAs) have recently emerged as important gene regulators in plants. MiRNAs and their targets have been extensively studied in Arabidopsis and rice. However, relatively little is known about the characterization of miRNAs and their target genes in peach (Prunus persica), which is a complex crop with unique developmental programs. Results: We performed small RNA deep sequencing and identified 47 peach-specific and 47 known miRNAs or families with distinct expression patterns. Together, the identified miRNAs targeted 80 genes, many of which have not been reported previously. Like the model plant systems, peach has two of the three conserved trans-acting siRNA biogenesis pathways with similar mechanistic features and target specificity. Unique to peach, three of the miRNAs collectively target 49 MYBs, 19 of which are known to regulate phenylpropanoid metabolism, a key pathway associated with stone hardening and fruit color development, highlighting a critical role of miRNAs in the regulation of peach fruit development and ripening. We also found that the majority of the miRNAs were differentially regulated in different tissues, in part due to differential processing of miRNA precursors. Up to 16% of the peach-specific miRNAs were differentially processed from their precursors in a tissue specific fashion, which has been rarely observed in plant cells. The miRNA precursor processing activity appeared not to be coupled with its transcriptional activity but rather acted independently in peach. Conclusions: Collectively, the data characterizes the unique expression pattern and processing regulation of peach miRNAs and demonstrates the presence of a complex, multi-level miRNA regulatory network capable of targeting a wide variety of biological functions, including phenylpropanoid pathways which play a multifaceted spatial-temporal role in peach fruit development. C1 [Zhu, Hong; Xia, Rui; Zhao, Bingyu; Liu, Zongrang] Virginia Polytech Inst & State Univ, Dept Hort, Blacksburg, VA 24061 USA. [Xia, Rui] Virginia Polytech Inst & State Univ, Alson H Smith Agr Res & Extens Ctr, Winchester, VA 22602 USA. [Zhu, Hong; Xia, Rui; Dardick, Chris D.; Callahan, Ann M.; Liu, Zongrang] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV 25430 USA. [An, Yong-qiang] USDA ARS, Danforth Plant Sci Ctr, St Louis, MO 63132 USA. RP Liu, ZR (reprint author), Virginia Polytech Inst & State Univ, Dept Hort, Blacksburg, VA 24061 USA. EM zongrang.liu@ars.usda.gov OI Xia, Rui/0000-0003-2409-1181 NR 77 TC 40 Z9 160 U1 7 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 21 PY 2012 VL 12 AR 149 DI 10.1186/1471-2229-12-149 PG 18 WC Plant Sciences SC Plant Sciences GA 072RA UT WOS:000313688700001 PM 22909020 ER PT J AU Cornman, RS Tarpy, DR Chen, YP Jeffreys, L Lopez, D Pettis, JS vanEngelsdorp, D Evans, D AF Cornman, R. Scott Tarpy, David R. Chen, Yanping Jeffreys, Lacey Lopez, Dawn Pettis, Jeffery S. vanEngelsdorp, Dennis Evans, D. TI Pathogen Webs in Collapsing Honey Bee Colonies SO PLOS ONE LA English DT Article ID ACUTE PARALYSIS VIRUS; DICISTROVIRUS AFFECTING HONEYBEES; APIS-MELLIFERA; NOSEMA-CERANAE; UNITED-STATES; VARROA-DESTRUCTOR; SEQUENCES; DISORDER; GENOME; INFECTION AB Recent losses in honey bee colonies are unusual in their severity, geographical distribution, and, in some cases, failure to present recognized characteristics of known disease. Domesticated honey bees face numerous pests and pathogens, tempting hypotheses that colony collapses arise from exposure to new or resurgent pathogens. Here we explore the incidence and abundance of currently known honey bee pathogens in colonies suffering from Colony Collapse Disorder (CCD), otherwise weak colonies, and strong colonies from across the United States. Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies. Pathogen loads were highly covariant in CCD but not control hives, suggesting that CCD colonies rapidly become susceptible to a diverse set of pathogens, or that co-infections can act synergistically to produce the rapid depletion of workers that characterizes the disorder. We also tested workers from a CCD-free apiary to confirm that significant positive correlations among pathogen loads can develop at the level of individual bees and not merely as a secondary effect of CCD. This observation and other recent data highlight pathogen interactions as important components of bee disease. Finally, we used deep RNA sequencing to further characterize microbial diversity in CCD and non-CCD hives. We identified novel strains of the recently described Lake Sinai viruses (LSV) and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD. The results are discussed with respect to host-parasite interactions and other environmental stressors of honey bees. C1 [Cornman, R. Scott; Chen, Yanping; Lopez, Dawn; Pettis, Jeffery S.; Evans, D.] ARS, Bee Res Lab, USDA, Beltsville, MD USA. [Tarpy, David R.; Jeffreys, Lacey] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [vanEngelsdorp, Dennis] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. RP Cornman, RS (reprint author), ARS, Bee Res Lab, USDA, Beltsville, MD USA. EM Jay.evans@ars.usda.gov RI vanEngelsdorp, Dennis/E-7934-2010; OI Evans, Jay/0000-0002-0036-4651 FU National Honey Board; Pennsylvania Department of Agriculture; North American Pollinator Protection Campaign; Apiary Inspectors of America; USDA-CSREES Coordinated Agricultural Project for Pollinator Health FX This work was supported by the National Honey Board, the Pennsylvania Department of Agriculture, the North American Pollinator Protection Campaign, the Apiary Inspectors of America and the USDA-CSREES Coordinated Agricultural Project for Pollinator Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 110 Z9 113 U1 18 U2 303 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 21 PY 2012 VL 7 IS 8 AR e43562 DI 10.1371/journal.pone.0043562 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 992PA UT WOS:000307789700040 PM 22927991 ER PT J AU Liu, JK Tang, XF Gao, LY Gao, YF Li, YX Huang, SX Sun, XC Miao, M Zeng, H Tian, XF Niu, XL Zheng, L Giovannoni, J Xiao, FM Liu, YS AF Liu, Jikai Tang, Xiaofeng Gao, Lanyang Gao, Yongfeng Li, Yuxiang Huang, Shengxiong Sun, Xiaochun Miao, Min Zeng, Hui Tian, Xuefen Niu, Xiangli Zheng, Lei Giovannoni, Jim Xiao, Fangming Liu, Yongsheng TI A Role of Tomato UV-Damaged DNA Binding Protein 1 (DDB1) in Organ Size Control via an Epigenetic Manner SO PLOS ONE LA English DT Article ID SIGNAL-TRANSDUCTION; HISTONE H4; ARABIDOPSIS-THALIANA; PLANT DEVELOPMENT; FLOWERING TIME; FRUIT-GROWTH; METHYLTRANSFERASE; METHYLATION; GENE; INTERACTS AB Epigenetic modification generally refers to phenotypic changes by a mechanism other than changes in DNA sequence and plays a significant role in developmental processes. In this study, we found that overexpression of one alternatively spliced tomato DDB1 transcript, DDB1 F that is prevalently present in all tested tissues, resulted in reduction of organ size. Transgenic plants constitutively expressing the DDB1 F from a strong cauliflower mosaic virus (CaMV) 35S promoter displayed moderately reduced size in vegetative organs (leaves and stems) and radically decreased size in reproductive organs (flowers, seeds and fruits), in which several genes encoding negative regulators for cell division were upregulated. Significantly, reduction of organ size conferred by overexpression of DDB1 F transgene appears not to segregate in the subsequent generations, suggesting the phenotypic alternations are manipulated in an epigenetic manner and can be transmitted over generations. This notion was further substantiated by analysis of DNA methylation level at the SlWEE1 gene (encoding a negative regulator of cell division), revealing a correlation between less methylation in the promoter region and elevated expression level of this gene. Thus, our results suggest DDB1 plays an important role in regulation of the epigenetic state of genes involved in organogenesis, despite the underlying mechanism remains to be elucidated. C1 [Liu, Jikai; Tang, Xiaofeng; Gao, Lanyang; Gao, Yongfeng; Li, Yuxiang; Huang, Shengxiong; Sun, Xiaochun; Miao, Min; Zeng, Hui; Tian, Xuefen; Liu, Yongsheng] Sichuan Univ, Minist Educ, State Key Lab Hydraul & Mt River Engn, Key Lab Bioresource & Ecoenvironm,Coll Life Sci, Chengdu 610064, Peoples R China. [Liu, Jikai; Tang, Xiaofeng; Niu, Xiangli; Zheng, Lei; Liu, Yongsheng] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei, Peoples R China. [Miao, Min; Xiao, Fangming] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Giovannoni, Jim] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Giovannoni, Jim] Cornell Univ, USDA ARS, Robert Holly Ctr, Ithaca, NY 14853 USA. RP Liu, JK (reprint author), Sichuan Univ, Minist Educ, State Key Lab Hydraul & Mt River Engn, Key Lab Bioresource & Ecoenvironm,Coll Life Sci, Chengdu 610064, Peoples R China. EM fxiao@uidaho.edu; liuyongsheng1122@yahoo.com.cn FU National Science Fund for Distinguished Young Scholars [30825030]; National Natural Science Foundation of China [31171179]; National Science and Technology Key Project of China [2009ZX08001-011B, 2009ZX08009-072B, 2011CB100401]; University of Idaho Startup Funding FX This work was supported by the National Science Fund for Distinguished Young Scholars (No. 30825030), the National Natural Science Foundation of China (No. 31171179), the National Science and Technology Key Project of China (Nos. 2009ZX08001-011B, 2009ZX08009-072B and 2011CB100401) to Y. Liu and the University of Idaho Startup Funding for F. Xiao. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 7 Z9 8 U1 2 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 21 PY 2012 VL 7 IS 8 AR e42621 DI 10.1371/journal.pone.0042621 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 992PA UT WOS:000307789700014 PM 22927934 ER PT J AU Lemly, AD Skorupa, JP AF Lemly, A. Dennis Skorupa, Joseph P. TI Wildlife and the Coal Waste Policy Debate: Proposed Rules for Coal Waste Disposal Ignore Lessons from 45 Years of Wildlife Poisoning SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article AB This analysis examines wildlife poisoning from coal combustion waste (CCW) in the context of EPA's proposed policy that would allow continued use of surface impoundments as a disposal method. Data from 21 confirmed damage sites were evaluated, ranging from locations where historic poisoning has led to corrective actions that have greatly improved environmental conditions to those where contamination has just recently been discovered and the level of ecological impacts has yet to be determined. The combined direct and indirect cost of poisoned fish and wildlife exceeds $2.3 billion, which is enough money to construct 155 landfills with state-of-the-art composite liners and leachate collection systems. This cost is projected to increase by an additional $3.85 billion over the next 50 years, an amount that would construct 257 landfills. Evidence revealed through this study indicates the following: (1) for the past 45 years, environmental damage has been a recurring theme with surface impoundment of CCW, (2) the National Pollutant Discharge Elimination System has not been effective in preventing serious environmental damage from CCW, (3) EPA's Regulatory Impact Analysis of the costs and benefits of pollution control options fails to include benefits of avoided damages to natural resources, specifically, poisoned fish and wildlife, and (4) surface impoundments pose unacceptably high ecological risks regardless of location or design. Regulators should no longer ignore rigorous science and the lessons from multiple case examples. EPA and the United States need to show leadership on this issue by prohibiting surface impoundments, particularly since the rise in coal use in developing countries is leading to the same CCW pollution problems on a global scale. C1 [Lemly, A. Dennis] Wake Forest Univ, US Forest Serv, USDA, So Res Stn,Piedmont Aquat Res Lab,Dept Biol, Winston Salem, NC 27109 USA. [Skorupa, Joseph P.] US Fish & Wildlife Serv, Div Environm Qual, Arlington, VA 22203 USA. RP Lemly, AD (reprint author), Wake Forest Univ, US Forest Serv, USDA, So Res Stn,Piedmont Aquat Res Lab,Dept Biol, Winston Salem, NC 27109 USA. EM dlemly@fs.fed.us NR 30 TC 24 Z9 24 U1 3 U2 25 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 21 PY 2012 VL 46 IS 16 BP 8595 EP 8600 DI 10.1021/es301467q PG 6 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 991JQ UT WOS:000307697700008 PM 22839645 ER PT J AU Wu, L Gao, B Munoz-Carpena, R Pachepsky, YA AF Wu, Lei Gao, Bin Munoz-Carpena, Rafael Pachepsky, Yakov A. TI Single Collector Attachment Efficiency of Colloid Capture by a Cylindrical Collector in Laminar Overland Flow SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID UNFAVORABLE DEPOSITION CONDITIONS; SATURATED POROUS-MEDIA; PREDICTING FILTER COEFFICIENT; SECONDARY-MINIMUM DEPOSITION; PARTICLE-SIZE; CORRELATION EQUATION; SURFACE INTERACTIONS; CHEMICAL CONDITIONS; BROWNIAN PARTICLES; ORGANIC-MATTER AB Little research has been conducted to investigate the fate and transport of colloids in shallow overland flow through dense vegetation under unfavorable chemical conditions. In this work, the single collector attachment efficiency (a) of colloid capture by a simulated plant stem (i.e., cylindrical collector) in laminar overland flow was measured directly in laboratory flow chamber experiments. Fluorescent microspheres of two sizes were used as experimental colloids. The colloid suspensions flowed toward a glass cylindrical rod installed in a small size flow channel at different laminar flow rates. Different solution ionic strengths (IS) were used in the experiments to simulate unfavorable attachment conditions. Our results showed that a increased with IS and decreased with flow velocity. Existing theoretical and empirical models of colloid attachment efficiency for porous media were used to simulate the experimental measurements in a and found to fall short in matching the experimental data. A new dimensionless (regression) equation was proposed that predicts the a of colloid capture by a cylindrical collector in laminar overland flow with reasonable accuracy. In addition, the equation was also effective in predicting the attachment efficiency of colloid deposition in porous media. C1 [Wu, Lei; Gao, Bin; Munoz-Carpena, Rafael] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Pachepsky, Yakov A.] ARS, USDA, Environm Microbial Safety Lab, Beltsville, MD 20705 USA. RP Gao, B (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM bg55@ufl.edu; carpena@ufl.edu RI Munoz-Carpena, Rafael/A-7588-2010; Wu, Lei/B-7129-2015; Gao, Bin/B-8027-2010; OI Munoz-Carpena, Rafael/0000-0003-2838-1514; Gao, Bin/0000-0003-3769-0191; Pachepsky, Yakov/0000-0003-0232-6090 NR 57 TC 6 Z9 6 U1 5 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 21 PY 2012 VL 46 IS 16 BP 8878 EP 8886 DI 10.1021/es301365f PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 991JQ UT WOS:000307697700042 PM 22799594 ER PT J AU Allen, PJ Batra, P Geiger, BM Wommack, T Gilhooly, C Pothos, EN AF Allen, Patricia J. Batra, Payal Geiger, Brenda M. Wommack, Tara Gilhooly, Cheryl Pothos, Emmanuel N. TI Rationale and consequences of reclassifying obesity as an addictive disorder: Neurobiology, food environment and social policy perspectives SO PHYSIOLOGY & BEHAVIOR LA English DT Review DE Addictive behavior; DSM IV; Food reward; Hyperphagia; Energy balance; Metabolic disorders ID HIGH-FAT DIET; DECREASES EXTRACELLULAR DOPAMINE; INCENTIVE-SENSITIZATION THEORY; BEHAVIOR-ASSOCIATED CHANGES; EXCESSIVE SUGAR INTAKE; BODY-MASS INDEX; NUCLEUS-ACCUMBENS; WEIGHT-LOSS; EATING BEHAVIOR; UNITED-STATES AB The rapid increase in the prevalence of obesity is a priority for investigators from across numerous disciplines, including biology, nutritional science, and public health and policy. In this paper, we systematically examine the premise that common dietary obesity is an addictive disorder, based on the criteria for addiction described in the Diagnostic and Statistical manual (DSM) of Mental Disorders of the American Psychiatric Association, version IV, and consider the consequences of such a reclassification of obesity for public policy. Specifically, we discuss evidence from both human and animal studies investigating the effects of various types and amounts of food and the food environment in obese individuals. Neurobiological studies have shown that the hedonic brain pathways activated by palatable food overlap considerably with those activated by drugs of abuse and suffer significant deficits after chronic exposure to high-energy diets. Furthermore, food as a stimulus can induce the sensitization, compulsion and relapse patterns observed in individuals who are addicted to illicit drugs. The current food environment encourages these addictive-like behaviors where increased exposure through advertisements, proximity and increased portion sizes are routine. Taking lessons from the tobacco experience, it is clear that reclassifying common dietary obesity as an addictive disorder would necessitate policy changes (e.g., regulatory efforts. economic strategies, and educational approaches). These policies could be instrumental in addressing the obesity epidemic, by encouraging the food industry and the political leadership to collaborate with the scientific and medical community in establishing new and more effective therapeutic approaches. (C) 2012 Elsevier Inc. All rights reserved. C1 [Geiger, Brenda M.; Pothos, Emmanuel N.] Tufts Univ, Sch Med, Dept Mol Physiol & Pharmacol, Boston, MA 02111 USA. [Allen, Patricia J.] Tufts Univ, Dept Psychol, Medford, MA 02155 USA. [Batra, Payal; Wommack, Tara; Gilhooly, Cheryl] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Batra, Payal; Wommack, Tara; Gilhooly, Cheryl] Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Geiger, Brenda M.; Pothos, Emmanuel N.] Tufts Univ, Sackler Sch Grad Biomed Sci, Grad Program Pharmacol & Expt Therapeut, Boston, MA 02111 USA. [Pothos, Emmanuel N.] Tufts Univ, Grad Program Neurosci, Sackler Sch Grad Biomed Sci, Boston, MA 02111 USA. RP Pothos, EN (reprint author), Tufts Univ, Sch Med, Dept Mol Physiol & Pharmacol, Med & Vet Bldg,Room 201,136 Harrison Ave, Boston, MA 02111 USA. EM Emmanuel.Pothos@tufts.edu OI Pothos, Emmanuel/0000-0001-8825-7852 FU National Institutes of Health [DK065872, ARRA 3R01DK065872, F31DA023760]; Smith Family Foundation Award of Excellence in Biomedical Research FX This article was produced as part of the First Tufts University Seminar on the Obesity Epidemic and Food Economics that was organized by Drs. Emmanuel N. Pothos (Chair), Robin B. Kanarek and Susan B. Roberts in Boston and Medford, MA. Part of the experimental evidence presented in this article was made possible through the generous support of the National Institutes of Health (DK065872, ARRA 3R01DK065872, F31DA023760) and a Smith Family Foundation Award of Excellence in Biomedical Research (ENP). NR 196 TC 12 Z9 12 U1 9 U2 49 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD AUG 20 PY 2012 VL 107 IS 1 BP 126 EP 137 DI 10.1016/j.physbeh.2012.05.005 PG 12 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA 001HA UT WOS:000308450000020 PM 22583861 ER PT J AU Beechy, L Galpern, J Petrone, A Das, SK AF Beechy, Laura Galpern, Jennie Petrone, Andrew Das, Sai Krupa TI Assessment tools in obesity - Psychological measures, diet, activity, and body composition SO PHYSIOLOGY & BEHAVIOR LA English DT Review DE Obesity; Psychometrics; Psychological tools; Dietary assessment; Body composition measurement ID QUALITY-OF-LIFE; BIOELECTRICAL-IMPEDANCE ANALYSIS; 3-FACTOR EATING QUESTIONNAIRE; DOUBLY LABELED WATER; EPWORTH SLEEPINESS SCALE; MULTIPLE-PASS METHOD; AIR DISPLACEMENT PLETHYSMOGRAPHY; PHYSICAL-ACTIVITY QUESTIONNAIRE; FOOD FREQUENCY QUESTIONNAIRES; PERSONAL DIGITAL ASSISTANT AB The global increase in the prevalence of obesity has led to an increased need for measurement tools for research, management and treatment of the obese person. The physical size limitations imposed by obesity, variations in body composition from that of normal weight, and a complex psychopathology all pose tremendous challenges to the assessment of an obese person. There is little published research regarding what tools can be used with confidence. This review is designed to provide researchers and clinicians with a guide to the current and emerging measurement tools specifically associated with obesity research and practice. Section 1 addresses psychological measures of well being. Sections 2, 3, and 4 focus on the assessment of food intake, activity, and body composition. All sections address basic challenges involved in the study and management of obesity, and highlight methodological issues associated with the use of common assessment tools. The best available methods for use in the obese both in research and clinical practice are recommended. (C) 2012 Elsevier Inc. All rights reserved. C1 [Das, Sai Krupa] Tufts Univ, Jean Mayer USDA HNRCA, Energy Metab Lab, Boston, MA 02111 USA. [Beechy, Laura; Galpern, Jennie; Das, Sai Krupa] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Petrone, Andrew] Tufts Univ, Sch Arts & Sci, Medford, MA 02155 USA. RP Das, SK (reprint author), Tufts Univ, Jean Mayer USDA HNRCA, Energy Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM sai.das@tufts.edu NR 235 TC 13 Z9 14 U1 6 U2 52 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD AUG 20 PY 2012 VL 107 IS 1 BP 154 EP 171 DI 10.1016/j.physbeh.2012.04.013 PG 18 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA 001HA UT WOS:000308450000023 PM 22548766 ER PT J AU Kaplan, F Zhao, W Richards, JT Wheeler, RM Guy, CL Levine, LH AF Kaplan, Fatma Zhao, Wei Richards, Jeffrey T. Wheeler, Raymond M. Guy, Charles L. Levine, Lanfang H. TI Transcriptional and Metabolic Insights into the Differential Physiological Responses of Arabidopsis to Optimal and Supraoptimal Atmospheric CO2 SO PLOS ONE LA English DT Article ID ELEVATED CARBON-DIOXIDE; GENE-EXPRESSION; PHOTOSYNTHETIC ACCLIMATION; STOMATAL CONDUCTANCE; SIGNAL-TRANSDUCTION; RISING CO2; PLANTS; GROWTH; SUGAR; THALIANA AB Background: In tightly closed human habitats such as space stations, locations near volcano vents and closed culture vessels, atmospheric CO2 concentration may be 10 to 20 times greater than Earth's current ambient levels. It is known that super-elevated (SE) CO2 (>1,200 mu mol mol(-1)) induces physiological responses different from that of moderately elevated CO2 (up to 1,200 mu mol mol(-1)), but little is known about the molecular responses of plants to supra-optimal [CO2]. Methodology/Principal Findings: To understand the underlying molecular causes for differential physiological responses, metabolite and transcript profiles were analyzed in aerial tissue of Arabidopsis plants, which were grown under ambient atmospheric CO2 (400 mmol mol(-1)), elevated CO2 (1,200 mmol mol(-1)) and SE CO2 (4,000 mu mol mol(-1)), at two developmental stages early and late vegetative stage. Transcript and metabolite profiling revealed very different responses to elevated versus SE [CO2]. The transcript profiles of SE CO2 treated plants were closer to that of the control. Development stage had a clear effect on plant molecular response to elevated and SE [CO2]. Photosynthetic acclimation in terms of down-regulation of photosynthetic gene expression was observed in response to elevated [CO2], but not that of SE [CO2] providing the first molecular evidence that there appears to be a fundamental disparity in the way plants respond to elevated and SE [CO2]. Although starch accumulation was induced by both elevated and SE [CO2], the increase was less at the late vegetative stage and accompanied by higher soluble sugar content suggesting an increased starch breakdown to meet sink strength resulting from the rapid growth demand. Furthermore, many of the elevated and SE CO2-responsive genes found in the present study are also regulated by plant hormone and stress. Conclusions/Significance: This study provides new insights into plant acclimation to elevated and SE [CO2] during development and how this relates to stress, sugar and hormone signaling. C1 [Kaplan, Fatma] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Zhao, Wei] MedImmune LLC, Gaithersburg, MD USA. [Richards, Jeffrey T.; Levine, Lanfang H.] Enterprise Advisory Serv Inc, QinetiQ N Amer Engn Serv Contract ESC, Sustainable Syst Appl Res, Kennedy Space Ctr, Kennedy Space Ctr, FL USA. [Wheeler, Raymond M.] NASA, Engn Directorate, Kennedy Space Ctr, Kennedy Space Ctr, FL USA. [Guy, Charles L.] Univ Florida, Inst Food & Agr Sci, Dept Environm Hort, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA. RP Kaplan, F (reprint author), Res LLC, Gainesville, FL USA. EM fkaplan@ufl.edu; lanfang.h.levine@nasa.gov NR 65 TC 24 Z9 25 U1 0 U2 32 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 20 PY 2012 VL 7 IS 8 AR e43583 DI 10.1371/journal.pone.0043583 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 991WJ UT WOS:000307733800079 PM 22916280 ER PT J AU Abbas, HK Bruns, A Weaver, MA Shier, WT AF Abbas, Hamed K. Bruns, Arnold Weaver, Mark A. Shier, Wayne T. TI Comparison of aflatoxin and fumonisin levels in corn from Bt and conventional hybrids SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Abbas, Hamed K.; Weaver, Mark A.] ARS, Biol Control Pests Res Unit, USDA, Stoneville, MS 38776 USA. [Bruns, Arnold] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. [Shier, Wayne T.] Univ Minnesota, Coll Pharm, Minneapolis, MN 55455 USA. EM hamed.abbas@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 319-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800566 ER PT J AU Ajwa, H Sullivan, D Gao, SD Stanghellini, M Sullivan, R AF Ajwa, Husein Sullivan, David Gao, Suduan Stanghellini, Mike Sullivan, Ryan TI Airborne flux of 1,3-dichloropropene and chloropicrin applied under totally impermeable film as a function of tarp cutting time SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Ajwa, Husein] Univ Calif Davis, Salinas, CA 93905 USA. [Sullivan, David; Sullivan, Ryan] Sullivan Environm, Alexandria, VA 22308 USA. [Gao, Suduan] USDA ARS, Parleir, CA 93648 USA. [Stanghellini, Mike] TriCal Inc, Hollister, CA 95024 USA. EM haajwa@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 85-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800346 ER PT J AU Aldema-Ramos, ML AF Aldema-Ramos, Mila L. TI Microbiological and near IR studies of leather from hides presoaked in formulations that can remove hardened bovine manure SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Aldema-Ramos, Mila L.] USDA, Wyndmoor, PA 19038 USA. EM mila.ramos@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 215-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800259 ER PT J AU Arvik, T Lipson, R Bartley, G Kim, H Yokoyama, W AF Arvik, Torey Lipson, Rebecca Bartley, Glenn Kim, Hyunsook Yokoyama, Wallace TI Chardonnay grape seed flour, not red grapes, reduce plasma cholesterol and body weight gain in hamsters on high fat diets SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Arvik, Torey; Lipson, Rebecca] Sonomaceuticals, Santa Rosa, CA 95402 USA. [Kim, Hyunsook] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Bartley, Glenn; Yokoyama, Wallace] Agr Res Serv, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM torey.arvik@sonomaceuticals.com NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 102-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800162 ER PT J AU Ashworth, DJ Yates, SR Luo, LF Lee, S Xuan, RC AF Ashworth, Daniel J. Yates, Scott R. Luo, Lifang Lee, Sang Xuan, Richeng TI Fate and transport of methyl iodide in raised beds under field conditions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Ashworth, Daniel J.; Luo, Lifang; Xuan, Richeng] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Yates, Scott R.; Lee, Sang] USDA ARS, Riverside, CA USA. EM daniel.ashworth@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 81-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800342 ER PT J AU Beck, JJ Higbee, BS Light, DM Gee, WS Hayashi, JM AF Beck, John J. Higbee, Bradley S. Light, Douglas M. Gee, Wai S. Hayashi, Jennifer M. TI Volatile natural products for monitoring a California tree nut insect pest SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Beck, John J.; Light, Douglas M.; Gee, Wai S.; Hayashi, Jennifer M.] ARS, Western Reg Res Ctr, Plant Mycotoxin Res Unit, USDA, Albany, CA 94710 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. EM john.beck@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 41-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800303 ER PT J AU Beck, JJ AF Beck, John J. TI Natural products chemistry and its applications to real-world agricultural problems SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Beck, John J.] ARS, Western Reg Res Ctr, Plant Mycol Res Unit, USDA, Albany, CA 94710 USA. EM john.beck@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 19-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800285 ER PT J AU Bernier, U Tabanca, N Khan, IA Blythe, EK Demirci, B Tsikolia, M Agramonte, NM Wedge, DE AF Bernier, Ulrich Tabanca, Nurhayat Khan, Ikhlas A. Blythe, Eugene K. Demirci, Betul Tsikolia, Maia Agramonte, Natasha M. Wedge, David E. TI Determination of repellent efficacy of natural compounds SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Bernier, Ulrich; Tsikolia, Maia; Agramonte, Natasha M.] ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, USDA, Gainesville, FL 32608 USA. [Tabanca, Nurhayat; Khan, Ikhlas A.; Wedge, David E.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Blythe, Eugene K.] Mississippi State Univ, South Mississippi Branch, Coastal Res & Extens Ctr, Expt Stn, Poplarville, MS 39470 USA. [Demirci, Betul] Anadolu Univ, Dept Pharmacognosy, Fac Pharm, Eskisehir, Turkey. EM uli.bernier@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 39-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800302 ER PT J AU Bernier, UR Tsikolia, M Agramonte, NM AF Bernier, Ulrich R. Tsikolia, Maia Agramonte, Natasha M. TI Development of acylpiperidine and carboxamide repellents using structure-activity models SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Bernier, Ulrich R.; Tsikolia, Maia; Agramonte, Natasha M.] USDA ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, Gainesville, FL 32608 USA. EM uli.bernier@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 151-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800405 ER PT J AU Bialek-Kalinski, K Rice, CP McCarty, GW AF Bialek-Kalinski, Krystyna Rice, Clifford P. McCarty, Gregory W. TI Herbicide washoff from forest canopy through-fall depends on rainfall dynamics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Bialek-Kalinski, Krystyna; Rice, Clifford P.; McCarty, Gregory W.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM krystyna.bialek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 195-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800448 ER PT J AU Boateng, AA Mullen, CA AF Boateng, Akwasi A. Mullen, Charles A. TI Pyrolysis-GC/MS of biomass thermally pretreated by torrefaction SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Boateng, Akwasi A.; Mullen, Charles A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 577-ENFL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804121 ER PT J AU Brown, EM Liu, CK AF Brown, Eleanor M. Liu, Cheng-Kung TI Collagen microfibril model: A tool for biomaterials scientists SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Brown, Eleanor M.; Liu, Cheng-Kung] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM ellie_brown@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 210-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621807871 ER PT J AU Cantrell, CL Ali, A Bernier, UR AF Cantrell, Charles L. Ali, Abbas Bernier, Ulrich R. TI Mosquito biting deterrence: Structure-activity relationship of saturated and unsaturated fatty acids SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Ali, Abbas] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Bernier, Ulrich R.] USDA ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, Gainesville, FL 32608 USA. EM clcantr1@olemiss.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 152-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800406 ER PT J AU Cantrell, CL Duke, SO Dayan, F AF Cantrell, Charles L. Duke, Stephen O. Dayan, Franck TI Natural products as sources for new pesticides SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cantrell, Charles L.; Duke, Stephen O.; Dayan, Franck] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. EM clcantr1@olemiss.edu NR 0 TC 0 Z9 0 U1 5 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 11-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800277 ER PT J AU Carroll, JF AF Carroll, John F. TI Natural product options for repelling ticks of medical importance SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Carroll, John F.] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. EM john.carroll@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 14-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800280 ER PT J AU Cavanaugh, JE Allen, E Rimando, AM Mizuno, CS AF Cavanaugh, Jane E. Allen, Erika Rimando, Agnes M. Mizuno, Cassia S. TI Reversal of age-related motor deficits following stilbene dietary supplementation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cavanaugh, Jane E.; Allen, Erika] Duquesne Univ, Grad Sch Pharmaceut Sci, Dept Pharmacol, Pittsburgh, PA 15282 USA. [Rimando, Agnes M.; Mizuno, Cassia S.] ARS, Dept Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. EM cavanaughj@duq.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 7-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800073 ER PT J AU Chaluvadi, S Hotchkiss, AT Call, JE Luchansky, JB Phillips, JG Liu, LS Yam, KL AF Chaluvadi, Saikiran Hotchkiss, Arland T. Call, Jeffrey E. Luchansky, John B. Phillips, John G. Liu, LinShu Yam, Kit L. TI Survival of probiotic bacteria within a synbiotic matrix SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Chaluvadi, Saikiran; Yam, Kit L.] Rutgers State Univ, New Brunswick, NJ 08901 USA. [Hotchkiss, Arland T.; Call, Jeffrey E.; Luchansky, John B.; Liu, LinShu] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Phillips, John G.] ARS, USDA, North Atlantic Area Off, Wyndmoor, PA 19038 USA. EM scrutgers@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 40-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801413 ER PT J AU Chellemi, DO Ajwa, HA AF Chellemi, Dan O. Ajwa, Husein A. TI Contribution of good agricultural practices (GAPs) to soil fumigant emission reduction SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Chellemi, Dan O.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Ajwa, Husein A.] Univ Calif Davis, Dept Vegetable Crops, Salinas, CA 93905 USA. EM dan.chellemi@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 84-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800345 ER PT J AU Chen, GY AF Chen, Guoying TI Screening of fluoroquinolone residues in caprine milk using a portable time-resolved fluorometer SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Chen, Guoying] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM guoying.chen@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 195-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800239 ER PT J AU Cheng, HN Biswas, A AF Cheng, H. N. Biswas, Atanu TI Use of common beans as components in polymeric materials SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cheng, H. N.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Biswas, Atanu] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM hn.cheng@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 381-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621808537 ER PT J AU Cheng, HN Biswas, A AF Cheng, H. N. Biswas, Atanu TI Vegetable oil-based new materials SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cheng, H. N.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Biswas, Atanu] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM hncheng100@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 18-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621808199 ER PT J AU Cheng, QZ Tong, ZH Zhu, JY AF Cheng, Qingzheng (George) Tong, Zhaohui Zhu, Junyong TI Biobased materials from disc refinery and ultrasonic treated lignocellulose bagasse residues from simplified bioethanol process SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cheng, Qingzheng (George); Tong, Zhaohui] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Zhu, Junyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM qzhcheng@ufl.edu; ztong@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 3-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801383 ER PT J AU Cocker, DR Silva, PJ Purvis-Roberts, K Price, DJ Tang, XC Malloy, Q Erupe, ME Brown, RS AF Cocker, David R. Silva, Philip J. Purvis-Roberts, Katie Price, Derek J. Tang, Xiaochen Malloy, Quentin Erupe, Mark E. Brown, Robert S. TI Atmospheric reactivity studies of aliphatic amines SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cocker, David R.; Price, Derek J.; Tang, Xiaochen; Malloy, Quentin] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA. [Silva, Philip J.] USDA ARS, Bowling Green, KY 42104 USA. [Purvis-Roberts, Katie] Claremont Mckenna Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Purvis-Roberts, Katie] Pitzer Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Purvis-Roberts, Katie] Scripps Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Erupe, Mark E.; Brown, Robert S.] Utah State Univ, Logan, UT 84322 USA. EM phil.silva@ars.usda.gov RI Cocker, David/F-4442-2010 OI Cocker, David/0000-0002-0586-0769 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 219-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800469 ER PT J AU Compton, DL Laszlo, JA Appell, M Vermillion, KE Evans, KO AF Compton, David L. Laszlo, Jospeh A. Appell, Michael Vermillion, Karl E. Evans, Kervin O. TI 2-Monoacylglycerol acyl migration: Effect of fatty acid desaturation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Compton, David L.; Laszlo, Jospeh A.; Appell, Michael; Vermillion, Karl E.; Evans, Kervin O.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM david.compton@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 127-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800183 ER PT J AU Cote, GL Skory, CD AF Cote, Gregory L. Skory, Christopher D. TI Glucansucrases from lactic acid bacteria which produce water-insoluble polysaccharides from sucrose SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Cote, Gregory L.; Skory, Christopher D.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM greg.cote@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 34-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801407 ER PT J AU de Guzman, GTN Codling, EE Prabhakara, K Hapeman, CJ Hively, WD McCarty, GW Lang, MW Torrents, A AF de Guzman, Gabriela T. Nino Codling, Eton E. Prabhakara, Kusuma Hapeman, Cathleen J. Hively, W. Dean McCarty, Gregory W. Lang, Megan W. Torrents, Alba TI Potential arsenic sources in a Choptank River subwatershed and the influence of land use and watershed characteristics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [de Guzman, Gabriela T. Nino; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Codling, Eton E.; Hapeman, Cathleen J.; McCarty, Gregory W.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Prabhakara, Kusuma] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Hively, W. Dean] US Geol Survey, Reston, VA 20191 USA. [Lang, Megan W.] US Forest Serv, USDA, Beltsville, MD 20705 USA. EM gndg@umd.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 197-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800450 ER PT J AU Dickens, JC AF Dickens, Joseph C. TI Mode of action of insect repellents SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Dickens, Joseph C.] USDA ARS, Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 153-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800407 ER PT J AU Duke, SO AF Duke, Stephen O. TI Clues to new herbicide modes of action from natural compounds SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Duke, Stephen O.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. EM stephen.duke@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 93-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800354 ER PT J AU Fan, XT Yun, J Li, XH AF Fan, Xuetong Yun, Juan Li, Xihong TI Use of gaseous essential oils to control foodborne human pathogenic bacteria on fresh produce SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Fan, Xuetong; Yun, Juan] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Yun, Juan; Li, Xihong] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Tianjin, Peoples R China. EM xuetong.fan@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 191-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800235 ER PT J AU Fan, XT Sokorai, K Engemann, J Gurtler, J Liu, YH AF Fan, Xuetong Sokorai, Kimberly Engemann, Juergen Gurtler, Joshua Liu, Yanhong TI In-package inactivation of Listeria innocua, Salmonella Typhimurium, and Escherichia coli O157:H7 on cherry tomatoes with gaseous ozone SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Fan, Xuetong; Sokorai, Kimberly; Gurtler, Joshua; Liu, Yanhong] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19454 USA. [Engemann, Juergen] JE PlasmaConsult GmbH, Wuppertal, Germany. EM xuetong.fan@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 35-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800101 ER PT J AU Fenn, ME AF Fenn, Mark E. TI Environmental and ecological effects of atmospheric nitrogen deposition SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Fenn, Mark E.] US Forest Serv, Pacific Southwest Res Stn, USDA, Riverside, CA 92507 USA. EM mfenn@fs.fed.us NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 216-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800466 ER PT J AU Fishman, ML Chau, HK Hotchkiss, AT Yadav, MP AF Fishman, Marshall L. Chau, Hoa K. Hotchkiss, Arland T., Jr. Yadav, Madhav P. TI Physico-chemical characterization of protein associated sugar beet pectin SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Fishman, Marshall L.; Chau, Hoa K.; Hotchkiss, Arland T., Jr.; Yadav, Madhav P.] ARS, Dept Dairy & Funct Foods, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM marshall.fishman@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 51-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801424 ER PT J AU Friday, D Mukkara, P Owens, R Baumstark, T Bruist, M AF Friday, Dillon Mukkara, Priyadarshini Owens, Robert Baumstark, Tilman Bruist, Michael TI Processing of potato spindle tuber viroid RNAs in host plant and non-host yeast SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Friday, Dillon; Mukkara, Priyadarshini; Bruist, Michael] Allegheny Univ Hlth Sci, Philadelphia, PA 19102 USA. [Owens, Robert] ARS, USDA, Beltsville, MD USA. [Baumstark, Tilman] Cornell Univ, Grad Sch Adm, Ithaca, NY 14853 USA. EM dfriday@mail.usp.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 83-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800653 ER PT J AU Galant, A Widmer, W Luzio, G Cameron, R AF Galant, Ashley Widmer, Wilbur Luzio, Gary Cameron, Randall TI Characterization of pectin from Citrus sinensis (sweet orange) juice SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Galant, Ashley; Widmer, Wilbur; Luzio, Gary; Cameron, Randall] USDA, Citrus & Subtrop Prod Res Unit, Ft Pierce, FL 34945 USA. EM ashley.galant@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 120-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800177 ER PT J AU Galli, RL Shukitt-Hale, B Bielinski, D AF Galli, Rachel L. Shukitt-Hale, Barbara Bielinski, Donna TI Effects of raspberry supplementation on measures of motor and cognitive function in aged rats SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Galli, Rachel L.] Simmons Coll, Dept Psychol, Boston, MA 02115 USA. [Shukitt-Hale, Barbara; Bielinski, Donna] Tufts Univ, USDA HNRCA, Boston, MA 02111 USA. EM galli@simmons.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 68-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800132 ER PT J AU Gao, SD Qin, RJ Ajwa, H Sullivan, DA Stanghellini, MS AF Gao, Suduan Qin, Ruijun Ajwa, Husein Sullivan, Dave A. Stanghellini, Michael S. TI Emission and movement of 1,3-dichloropropene and chloropicrin in a large field tarped with low permeability film SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Gao, Suduan] USDA ARS, Parlier, CA 93648 USA. [Qin, Ruijun; Ajwa, Husein] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Sullivan, Dave A.] Sullivan Environm Consulting Inc, Alexandria, VA 22308 USA. [Stanghellini, Michael S.] TriCal Inc, Hollister, CA 95023 USA. EM Suduan.Gao@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 86-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800347 ER PT J AU Geis-Asteggiante, L Lehotay, SJ Lightfield, AR AF Geis-Asteggiante, Lucia Lehotay, Steven J. Lightfield, Alan R. TI Ruggedness testing and validation of a practical method for more than 100 veterinary drug residues in bovine muscle by UHPLC-MS/MS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Geis-Asteggiante, Lucia; Lehotay, Steven J.; Lightfield, Alan R.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Geis-Asteggiante, Lucia] Univ Republica, Catedra Farmacognosia & Prod Nat, Montevideo 12800, Uruguay. EM lucia.geis@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 174-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800427 ER PT J AU Gish, T Prueger, J Kustas, W McKee, L Russ, A Hatfield, J AF Gish, Timothy Prueger, John Kustas, William McKee, Lynn Russ, Andrew Hatfield, Jerry TI Thirteen-year summary of field-scale herbicide volatilization SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Gish, Timothy; Kustas, William; McKee, Lynn; Russ, Andrew] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Prueger, John; Hatfield, Jerry] ARS, Natl Lab Agr & Environm, USDA, Ames, IA USA. EM timothy.gish@ars.usda.gov RI Kustas, William/C-2063-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 58-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800320 ER PT J AU Hafner, SD Meisinger, JJ Mulbry, W Ingram, SK AF Hafner, Sasha D. Meisinger, John J. Mulbry, Walter Ingram, Shannon K. TI pH-based method for measuring gaseous ammonia SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hafner, Sasha D.] USDA ARS, University Pk, PA 16802 USA. [Meisinger, John J.; Mulbry, Walter; Ingram, Shannon K.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM sdh11@cornell.edu RI Hafner, Sasha/N-3970-2013 OI Hafner, Sasha/0000-0003-0955-0327 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 207-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800457 ER PT J AU Hafner, SD Montes, F Green, P Howard, CJ Franco, RB Mitloehner, F Rotz, CA AF Hafner, Sasha D. Montes, Felipe Green, Peter Howard, Cody J. Franco, Roberta B. Mitloehner, Frank Rotz, C. Alan TI VOC emission from silage: Compounds, processes, and implications SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hafner, Sasha D.; Montes, Felipe; Rotz, C. Alan] ARS, USDA, University Pk, PA 16802 USA. [Green, Peter] Univ Calif Davis, Dept Civil & Environm Engn, Davis, CA 95616 USA. [Howard, Cody J.] US EPA, Natl Ctr Environm Res, Washington, DC 20460 USA. [Franco, Roberta B.; Mitloehner, Frank] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. EM sasha.hafner@gmail.com RI Hafner, Sasha/N-3970-2013 OI Hafner, Sasha/0000-0003-0955-0327 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 25-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800290 ER PT J AU Hakk, H Huwe, JK AF Hakk, Heldur Huwe, Janice K. TI Comparative metabolism of [C-14]a-, b-, and g-hexabromocyclododecane (HBCD) in rats SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hakk, Heldur; Huwe, Janice K.] ARS, Biosci Res Lab, USDA, Fargo, ND 58102 USA. EM heldur.hakk@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 26-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800291 ER PT J AU Hammer, NL Boateng, AA Mullen, CA AF Hammer, Nicole L. Boateng, Akwasi A. Mullen, Charles A. TI ASPEN PLUS modeling of the utilization of horse manure for localized power via fast pyrolysis SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hammer, Nicole L.; Boateng, Akwasi A.; Mullen, Charles A.] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM nicole.hammer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 475-ENFL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804029 ER PT J AU Hapeman, CJ McCarty, GW Rice, CP Hively, WD McConnell, LL Sadeghi, AM Lang, MW Whitall, DR Bialek, K Downey, P AF Hapeman, Cathleen J. McCarty, Gregory W. Rice, Clifford P. Hively, W. Dean McConnell, Laura L. Sadeghi, Ali M. Lang, Megan W. Whitall, David R. Bialek, Krystyna Downey, Peter TI Exploring the association of the metolachlor metabolite MESA and agricultural nitrate-N fate and transport in the Choptank River Watershed SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hapeman, Cathleen J.; McCarty, Gregory W.; Rice, Clifford P.; McConnell, Laura L.; Sadeghi, Ali M.; Bialek, Krystyna; Downey, Peter] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Hively, W. Dean] US Geol Survey, Eastern Sci Res Ctr, Reston, VA 22092 USA. [Lang, Megan W.] US Forest Serv, No Res Stn, USDA, Beltsville, MD USA. [Whitall, David R.] Natl Oceanog & Atmospher Adm, Natl Ctr Coastal Ocean Sci, Ctr Coastal Monitoring & Assessment, Silver Spring, MD USA. EM cathleen.hapeman@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 196-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800449 ER PT J AU Hapeman, CJ McConnell, LL Potter, TL Harman-Fetcho, JA Schmidt, WF Rice, CP Schaffer, BA Curry, R AF Hapeman, Cathleen J. McConnell, Laura L. Potter, Thomas L. Harman-Fetcho, Jennifer A. Schmidt, Walter F. Rice, Clifford P. Schaffer, Bruce A. Curry, Richard TI Atmospheric transport of endosulfan to Everglades and Biscayne National Parks SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hapeman, Cathleen J.; McConnell, Laura L.; Harman-Fetcho, Jennifer A.; Schmidt, Walter F.; Rice, Clifford P.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Potter, Thomas L.] ARS, SE Watershed Res Lab, USDA, Tifton, GA 31793 USA. [Schaffer, Bruce A.] Univ Florida, Trop Res & Extens Stn, Homestead, FL 33031 USA. [Curry, Richard] Natl Pk Serv, Biscayne Natl Pk, Homestead, FL 33033 USA. EM laura.mcconnell@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 59-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800321 ER PT J AU Hasson, AS Ogunjemiyo, SO Trabue, S Ashkan, S Olea, C Middala, S Vu, K Scruggs, A Addala, LR Nana, L Steele, J Shelton, T AF Hasson, Alam S. Ogunjemiyo, Segun O. Trabue, Steven Ashkan, Shawn Olea, Catalina Middala, Srikar Vu, Kennedy Scruggs, Austen Addala, Laxmi R. Nana, Lucien Steele, Julie Shelton, Thomas TI Emissions of ozone precursors from a central California dairy facility SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hasson, Alam S.; Olea, Catalina; Middala, Srikar; Vu, Kennedy; Scruggs, Austen; Addala, Laxmi R.; Nana, Lucien; Steele, Julie] Calif State Univ Fresno, Dept Chem, Fresno, CA 93740 USA. [Ogunjemiyo, Segun O.; Shelton, Thomas] Calif State Univ Fresno, Dept Geog, Fresno, CA 93740 USA. [Trabue, Steven] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Ashkan, Shawn] Calif State Univ Fresno, Ctr Irrigat Technol, Fresno, CA 93740 USA. EM ahasson@csufresno.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 217-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800467 ER PT J AU Heighton, LP Codling, EE Rice, CP Schmidt, WF AF Heighton, Lynne P. Codling, Eton E. Rice, Clifford P. Schmidt, Walter F. TI Detecting selective ligand-transition metal affinity in environmental samples by electrospray ionization mass spectroscopy SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Heighton, Lynne P.] Univ Maryland, Dept Chem & Biochemisty, College Pk, MD 20742 USA. [Codling, Eton E.; Rice, Clifford P.] ARS, USDA, EMBUL, Beltsville, MD 20745 USA. [Schmidt, Walter F.] ARS, USDA, EMSSL, Beltsville, MD 20745 USA. EM heighton@umd.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 243-ANYL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801107 ER PT J AU Heighton, LP Codling, EE Rice, CP Schmidt, WF AF Heighton, Lynne P. Codling, Eton E. Rice, Clifford P. Schmidt, Walter F. TI Quantification of organic phosphate in soil samples by electrospray ionization mass spectroscopy SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Heighton, Lynne P.] Univ Maryland, Dept Chem & Biochemisty, College Pk, MD 20742 USA. [Codling, Eton E.; Rice, Clifford P.; Schmidt, Walter F.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20745 USA. EM heighton@umd.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 107-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800366 ER PT J AU Hernandez-Viezcas, JA Andrews, J Castillo-Michel, H Rico, C Ge, Y Priester, JH Mielke, R Horst, AM Moritz, SC An, YJ Zhao, L Espinosa, K Palmer, R Gardea-Torresdey, J Holden, PA Walker, SL Schimel, J Nisbet, R AF Hernandez-Viezcas, Jose A. Andrews, Joy Castillo-Michel, Hiram Rico, Cyrene Ge, Yuan Priester, John H. Mielke, Randall Horst, Allison M. Moritz, Shelly C. An, Youn-Joo Zhao, Lijuan Espinosa, Katherine Palmer, Reid Gardea-Torresdey, Jorge Holden, Patricia A. Walker, Sharon L. Schimel, Joshua Nisbet, Roger TI Synchrotron imaging of the uptake and speciation of ZnO and CeO2 in soil cultivated soybean (Glycine max) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hernandez-Viezcas, Jose A.; Gardea-Torresdey, Jorge] Univ Texas El Paso, El Paso, TX 79968 USA. [Ge, Yuan; Priester, John H.; Mielke, Randall; Horst, Allison M.] Univ Calif Santa Barbara, Bren Sch ESM, Santa Barbara, CA 93106 USA. [Ge, Yuan; Priester, John H.; Mielke, Randall; Horst, Allison M.; Moritz, Shelly C.; Holden, Patricia A.; Schimel, Joshua; Nisbet, Roger] Univ Calif Santa Barbara, Earth Res Inst, Santa Barbara, CA 93106 USA. [Schimel, Joshua; Nisbet, Roger] Univ Calif Santa Barbara, Santa Barbara, CA 93106 USA. [Andrews, Joy] Stanford Linear Accelerator Ctr, Menlo Pk, CA 94025 USA. [Castillo-Michel, Hiram] European Synchrotron Radiat Facil, F-38043 Grenoble, France. [Hernandez-Viezcas, Jose A.; Ge, Yuan; Priester, John H.; Mielke, Randall; Horst, Allison M.; Zhao, Lijuan; Gardea-Torresdey, Jorge; Holden, Patricia A.; Walker, Sharon L.; Schimel, Joshua; Nisbet, Roger] Univ Calif Santa Barbara, Ctr Environm Implicat Nanotechnol, Riverside, CA 92521 USA. [An, Youn-Joo] Konku Univ, Dept Environm Sci, Seoul, South Korea. [Espinosa, Katherine; Palmer, Reid] Iowa State Univ, Dept Agron, Ames, IA USA. [Palmer, Reid] USDA, NRS, Ames, IA USA. [Walker, Sharon L.] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA. EM jahernandez19@miners.utep.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 189-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804411 ER PT J AU Horak, K Chipman, R Murphy, L Johnston, JJ AF Horak, Katherine Chipman, Richard Murphy, Lisa Johnston, John J. TI Environmental contaminants in wildlife intended for human consumption SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Horak, Katherine] Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA. [Chipman, Richard] Anim & Plant Hlth Inspect Serv, Wildlife Serv, USDA, Castleton, NY 12033 USA. [Murphy, Lisa] Univ Penn, Penn Anim Diagnost Lab, Syst New Bolton Ctr, Toxicol Lab,Sch Vet Med, Kennett Sq, PA 19348 USA. [Johnston, John J.] Food Safety & Inspect Serv, Off Publ Hlth Sci, USDA, Ft Collins, CO 80526 USA. EM john.johnston@fsis.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 23-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800289 ER PT J AU Hotchkiss, AT AF Hotchkiss, Arland T. TI Antiadhesive activity of plant oligosaccharides SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Hotchkiss, Arland T.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM arland.hotchkiss@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 44-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801417 ER PT J AU Huang, Q McConnell, LL Razote, E Schmidt, WF Vinyard, B Torrents, A Hapeman, CJ Maghirang, R Trabue, S AF Huang, Qiang McConnell, Laura L. Razote, Edna Schmidt, Walter F. Vinyard, Bryan Torrents, Alba Hapeman, Cathleen J. Maghirang, Ronaldo Trabue, Steven TI Developing novel approaches to characterize emissions from agricultural operations SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Huang, Qiang; Torrents, Alba] Univ Maryland, College Pk, MD 20742 USA. [McConnell, Laura L.; Schmidt, Walter F.; Vinyard, Bryan; Hapeman, Cathleen J.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Razote, Edna; Maghirang, Ronaldo] Kansas State Univ, Dept Biol & Agr Engn, Manhattan, KS 66506 USA. [Trabue, Steven] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. EM laura.mcconnell@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 21-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800287 ER PT J AU Jackson, MA Cermak, SC AF Jackson, Michael A. Cermak, Steven C. TI Renewable 2-undecanone from Cuphea sp oil through a ketonization reaction SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Jackson, Michael A.; Cermak, Steven C.] ARS, USDA, NCAUR, Peoria, IL 61604 USA. EM michael.jackson@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 202-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800246 ER PT J AU Jin, TC Wang, Y Chen, YW Albillos, SM Kothary, MH Fu, TJ Tankersley, B McHugh, TH Zhang, YZ AF Jin, Tengchuan Wang, Yang Chen, Yu-Wei Albillos, Silvia M. Kothary, Mahendra H. Fu, Tong-Jen Tankersley, Boyce McHugh, Tara H. Zhang, Yu-Zhu TI Purification and characterization of convicilin, a vicilin-like globulin, from Korean pine (Pinus koraiensis) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [McHugh, Tara H.; Zhang, Yu-Zhu] USDA, ARS PWA WRRC, Albany, CA 94710 USA. [Jin, Tengchuan; Wang, Yang; Chen, Yu-Wei; Zhang, Yu-Zhu] IIT, Dept Biol & Chem Sci, Chicago, IL 60616 USA. [Fu, Tong-Jen] US FDA, Natl Ctr Food Safety & Technol, Bedford Pk, IL 60501 USA. [Kothary, Mahendra H.] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD 20708 USA. [Albillos, Silvia M.] IIT, Natl Ctr Food Safety & Technol, Bedford, IL 60501 USA. [Tankersley, Boyce] Chicago Bot Garden, Glencoe, IL 60022 USA. EM yuzhu.zhang@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 219-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800263 ER PT J AU Jin, TZ Sang, WG AF Jin, Tony Z. Sang, Weiguo TI Application of allyl isothiocyanate in foods for pathogen control SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Jin, Tony Z.] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA. [Sang, Weiguo] Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China. EM tony.jin@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 188-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800233 ER PT J AU Johnson, J Pettis, J AF Johnson, Josephine Pettis, Jeffery TI Survey of imidacloprid levels in water sources frequented by honey bees (Apis mellifera) in Maryland SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Johnson, Josephine] Univ Maryland, Sch Med, Dept Toxicol, Baltimore, MD 21201 USA. [Pettis, Jeffery] ARS, Bee Res Lab, USDA, Beltsville, MD 20705 USA. EM jdjohnso@epi.umaryland.edu NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 281-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800530 ER PT J AU Johnston, JJ Zhou, T AF Johnston, John J. Zhou, Tong TI Inter-agency risk communication: Inorganic arsenic residues in poultry SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Johnston, John J.] US Food Safety & Inspect Serv, Off Publ Hlth Sci, Off Assistant Administrator, USDA, Washington, DC 20250 USA. [Zhou, Tong] US FDA, Off New Anim Drug Evaluat, Div Human Food Safety, Ctr Vet Med, Rockville, MD 20855 USA. EM John.Johnston@fsis.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 267-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800516 ER PT J AU Jong, L AF Jong, Lei TI Effect of microfluidized and stearic acid modified soy protein in natural rubber SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Jong, Lei] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 239-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621808008 ER PT J AU Joseph, PM AF Joseph, Patt M. TI Role of terpenes and other stimuli in host-plant assessment by Asian citrus psyllid, vector of citrus greening disease SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Joseph, Patt M.] ARS, US Hort Res Lab, Subtrop Insects Res Unit, USDA, Ft Pierce, FL 34945 USA. EM joseph.patt@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 65-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800326 ER PT J AU Kim, S AF Kim, Sanghoon TI Production of poly(alkyl cyanoacrylate) nanoparticles as a coating material that changes wetting property SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Kim, Sanghoon] ARS, USDA, NCAUR, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 605-COLL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621802779 ER PT J AU Knighton, R AF Knighton, Raymond TI Trends in emissions of reactive nitrogen from US agriculture SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Knighton, Raymond] USDA, Inst Bioenergy Climate & Environm, Natl Inst Food & Agr, Washington, DC 20250 USA. EM rknighton@nifa.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 156-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800410 ER PT J AU Lakshman, DK Mitra, A AF Lakshman, Dilip K. Mitra, Amitava TI Lactoferrin imparts resistance against fungal pathogens in transgenic plants SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lakshman, Dilip K.] ARS, Floral & Nursery Res Unit, USDA, Beltsville, MD 20705 USA. [Mitra, Amitava] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. EM dilip.lakshman@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 320-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800567 ER PT J AU Lamont, MN AF Lamont, Martha N. TI Monitoring pesticides in foods: Responding to global market changes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lamont, Martha N.] Agr Mkt Serv, Pesticide Data Program, USDA, Manassas, VA 20110 USA. EM martha.lamont@ams.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 128-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800382 ER PT J AU Lee, J Dossett, M Finn, CE AF Lee, Jungmin Dossett, Michael Finn, Chad E. TI Next generation of superfruits: Enhancing anthocyanin rich black raspberries SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lee, Jungmin] ARS, Hort Crops Res Unit Worksite, USDA, Parma, ID 83660 USA. [Dossett, Michael] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Finn, Chad E.] ARS, Hort Crops Res Unit, USDA, Corvallis, OR 97330 USA. EM jungmin.lee@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 128-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800184 ER PT J AU Lehotay, SJ AF Lehotay, Steven J. TI What is good enough? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lehotay, Steven J.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 145-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800399 ER PT J AU Lipson, R Arvik, T Bartley, G Kim, H Yokoyama, W AF Lipson, Rebecca Arvik, Torey Bartley, Glenn Kim, Hyunsook Yokoyama, Wallace TI Chardonnay grape seed flour reduces plasma lipids and increases expression of hepatic genes for cholesterol and fat synthesis in hamsters fed a high fat diet SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lipson, Rebecca; Arvik, Torey] Sonomaceuticals, Santa Rosa, CA 95402 USA. [Kim, Hyunsook] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Bartley, Glenn; Yokoyama, Wallace] Agr Res Serv, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM rebecca.lipson@sonomaceuticals.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 100-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800160 ER PT J AU Liu, CK Latona, NP AF Liu, Cheng-Kung Latona, Nicholas P. TI Physical properties of collagen fibrous networks derived from bovine hides SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Liu, Cheng-Kung; Latona, Nicholas P.] ARS, USDA, Wyndmoor, PA 19038 USA. EM chengkung.liu@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 399-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621808156 ER PT J AU Liu, ZS AF Liu, Zengshe (Kevin) TI Study of soybean oil-based polymers for controlled release anticancer drugs SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Liu, Zengshe (Kevin)] ARS, BOR, NCAUR, USDA, Peoria, IL 61604 USA. EM kevin.liu@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 29-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801403 ER PT J AU Lundberg, B Hotchkiss, AT Pan, XJ AF Lundberg, Brock Hotchkiss, Arland T. Pan, Xuejun TI Characterization of citrus fiber rheology and physicochemical interactions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lundberg, Brock] Fiberstar Bioingredient Technol Inc, River Falls, WI 54022 USA. [Hotchkiss, Arland T.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Pan, Xuejun] Univ Wisconsin, Madison, WI 53706 USA. EM lundberg@fiberstarbio.com NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 50-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801423 ER PT J AU Luo, LF Yates, S Ashworth, D Simunek, J AF Luo, Lifang Yates, Scott Ashworth, Daniel Simunek, Jirka TI Predicting emission and distribution of fumigants applied via drip irrigation to raised beds SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Luo, Lifang; Yates, Scott; Ashworth, Daniel] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Luo, Lifang; Ashworth, Daniel; Simunek, Jirka] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92507 USA. EM lifang.luo@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 82-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800343 ER PT J AU Lupton, SJ Huwe, J Smith, DJ Dearfield, K Johnston, JJ AF Lupton, Sara J. Huwe, Janice Smith, David J. Dearfield, Kerry Johnston, John J. TI Absorption and distribution of perfluorooctane sulfonate (PFOS) in beef cattle SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Lupton, Sara J.; Huwe, Janice; Smith, David J.] ARS, Biosci Res Lab, USDA, Fargo, ND 58102 USA. [Dearfield, Kerry] Food Safety Inspect Serv, Off Publ Hlth Sci, USDA, Washington, DC 20520 USA. [Johnston, John J.] Food Safety Inspect Serv, Off Publ Hlth Sci, USDA, Ft Collins, CO 80526 USA. EM sara.lupton@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 28-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800293 ER PT J AU Manthey, JA Hijaz, FM Reyes-De-Corcuera, JR Folimonova, SY Davis, CL Jones, SE AF Manthey, John A. Hijaz, Faraj M. Reyes-De-Corcuera, Jose R. Folimonova, Svetlana Y. Davis, Craig L. Jones, Shelley E. TI HPLC-fluorescence studies of the changes in secondary metabolites during progression of Huanglonbing disease in sweet orange leaves SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Manthey, John A.] ARS, US Hort Res Lab, USDA, USHRL, Ft Pierce, FL 34945 USA. [Hijaz, Faraj M.; Reyes-De-Corcuera, Jose R.; Folimonova, Svetlana Y.; Davis, Craig L.; Jones, Shelley E.] Univ Florida, Inst Food & Agr Sci, Lake Alfred, FL USA. EM John.Manthey@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 183-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800229 ER PT J AU Mattoo, AK AF Mattoo, Autar K. TI Enhancing desirable nutrients in fruits and vegetables by transgenic approaches SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Mattoo, Autar K.] ARS, Beltsville Agr Res Ctr, Sustainable Agr Syst Lab, USDA, Beltsville, MD 20705 USA. EM autar.mattoo@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 293-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800541 ER PT J AU Medina, MB Li, QQ Yao, XM AF Medina, Marjorie B. Li, Qiongqiong Yao, Xiaomin TI Biosensor detection of staphylococcal enterotoxin A and B in cultures after removal of Protein A with immuno-affinity chromatography SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Medina, Marjorie B.; Li, Qiongqiong] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Li, Qiongqiong; Yao, Xiaomin] Shanghai Jiao Tong Univ, Dept Food Sci & Technol, Bor S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China. EM Melissa.Li@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 147-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800203 ER PT J AU Medina, MB Li, QQ AF Medina, Marjorie B. Li, Qiongqiong TI Fast Blue BB microassay method for determination of total phenolics in beverages and fruits SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Medina, Marjorie B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Li, Qiongqiong] Shanghai Jiao Tong Univ, Dept Food Sci & Technol, Bor S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China. EM marjorie.medina@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 83-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800145 ER PT J AU Meepagala, KM Osbrink, W Bernier, U Burandt, C Lax, A Becnel, J Sturtz, G Duke, SO AF Meepagala, Kumudini M. Osbrink, Weste Bernier, Ulrich Burandt, Charles Lax, Alan Becnel, James Sturtz, George Duke, Stephen O. TI Natural products from plants and their synthetic analogs against pests SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Meepagala, Kumudini M.; Duke, Stephen O.] Univ Mississippi, USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Osbrink, Weste; Lax, Alan] USDA ARS, So Reg Res Ctr, Formosan Subterranean Termite Res Unit, New Orleans, LA 70124 USA. [Bernier, Ulrich; Becnel, James] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Burandt, Charles] Univ Mississippi, University, MS 38677 USA. [Sturtz, George] Aromagen, Albany, OR 97321 USA. EM kmeepaga@olemiss.edu NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 68-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800329 ER PT J AU Mikulski, N AF Mikulski, Neil TI Foreign Agricultural Service: Linking US agriculture to the world SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Mikulski, Neil] Foreign Agr Serv, USDA, Washington, DC 20250 USA. EM Neil.Mikulski@fas.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 49-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800311 ER PT J AU Moreau, RA Johnston, DB Hicks, KB AF Moreau, Robert A. Johnston, David B. Hicks, Kevin B. TI Corn oil as a co-product of fuel ethanol production SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Moreau, Robert A.; Johnston, David B.; Hicks, Kevin B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM robert.moreau@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 298-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800545 ER PT J AU Mullen, CA Strahan, GD Boateng, AA AF Mullen, Charles A. Strahan, Gary D. Boateng, Akwasi A. TI Characterization of biomass fast pyrolysis oils using NMR and chemometrics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Mullen, Charles A.; Strahan, Gary D.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 299-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800546 ER PT J AU Natarajan, S AF Natarajan, Savithiry TI Assessment of the natural variation of soybean protein expression through proteomics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Natarajan, Savithiry] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM savi.natarajan@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 318-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800565 ER PT J AU Nghiem, NP Hicks, KB Johnston, DB AF Nghiem, Nhuan P. Hicks, Kevin B. Johnston, David B. TI Developing the biorefinery: Production of value-added products and fuel from lignocellulosic feedstocks SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Nghiem, Nhuan P.; Hicks, Kevin B.; Johnston, David B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM john.nghiem@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 296-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800543 ER PT J AU O'Keefe, M Dearfield, K Edwards, S Abdelmajid, N Blanchard, A LaBarre, D Bennett, P AF O'Keefe, Margaret Dearfield, Kerry Edwards, Sarah Abdelmajid, Naser Blanchard, Ashley LaBarre, David Bennett, Patricia TI FSIS activities regarding dioxin SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [O'Keefe, Margaret; Dearfield, Kerry; Edwards, Sarah; Abdelmajid, Naser; Blanchard, Ashley; LaBarre, David; Bennett, Patricia] USDA, Washington, DC 20250 USA. EM margaret.okeefe@fsis.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 36-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804264 ER PT J AU O'Keefe, M Abdelmajid, N Domesle, A Edwards, S LaBarre, D Bennett, P AF O'Keefe, Margaret Abdelmajid, Naser Domesle, Alexander Edwards, Sarah LaBarre, David Bennett, Patricia TI Enhancement of the United States National Residue Program for meat, poultry, and egg products SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [O'Keefe, Margaret; Abdelmajid, Naser; Domesle, Alexander; Edwards, Sarah; LaBarre, David; Bennett, Patricia] USDA, Washington, DC 20250 USA. EM margaret.okeefe@fsis.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 118-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800176 ER PT J AU Onwulata, C AF Onwulata, Charles TI Dissolution models for estimating glycemic potential of food materials SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Onwulata, Charles] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19012 USA. EM Charles.Onwulata@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 206-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800250 ER PT J AU Panickar, KS AF Panickar, Kiran S. TI Diverse cellular mechanisms underlying the neuroprotective effects of dietary polyphenols in ischemic injury in vitro SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Panickar, Kiran S.] USDA, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. EM kiran.panickar@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 9-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800075 ER PT J AU Papiernik, SK Yates, SR Qian, YR Ajwa, H Gao, SD Qin, RJ Sances, F Spokas, KA AF Papiernik, Sharon K. Yates, Scott R. Qian, Yaorong Ajwa, Husein Gao, Suduan Qin, Ruijun Sances, Frank Spokas, Kurt A. TI Interlaboratory validation of standardized method to determine permeability of plastic films SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Papiernik, Sharon K.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Yates, Scott R.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Qian, Yaorong] US EPA, Off Pesticide Programs, Ft George G Meade, MD 20755 USA. [Ajwa, Husein; Qin, Ruijun] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Gao, Suduan] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Sances, Frank] Pacific Ag Res, San Luis Obispo, CA 93401 USA. [Spokas, Kurt A.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. EM Sharon.Papiernik@ars.usda.gov RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 80-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800341 ER PT J AU Papiernik, SK Oliveira, RS Cabrera, A Koskinen, WC AF Papiernik, Sharon K. Oliveira, Rubem S. Cabrera, Alegria Koskinen, William C. TI Spatial variation in sorption and dissipation is herbicide dependent SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Papiernik, Sharon K.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Oliveira, Rubem S.] Univ Estadual Maringa, Dept Agron, BR-87020900 Maringa, Parana, Brazil. [Cabrera, Alegria] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Koskinen, William C.] USDA ARS, Soil & Water Management Unit, St Paul, MN 55108 USA. EM Sharon.Papiernik@ars.usda.gov RI Cabrera, Alegria/B-4753-2012 OI Cabrera, Alegria/0000-0002-0421-3324 NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 6-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800272 ER PT J AU Paul, M Van Hekken, DL AF Paul, Moushumi Van Hekken, Diane L. TI Antioxidative activity of organic vs. conventional milk SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Paul, Moushumi; Van Hekken, Diane L.] ARS, Dairy & Funct Foods Res Unit, USDA, NAA,ERRC, Wyndmoor, PA 19038 USA. EM moushumi.paul@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 124-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800180 ER PT J AU Pettis, J Johnson, J AF Pettis, Jeffery Johnson, Josephine TI Movement of soil and trunk injected imidacloprid through the hardwood Acer rubrum SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Pettis, Jeffery] ARS, Bee Res Lab, USDA, Beltsville, MD 20705 USA. [Johnson, Josephine] Univ Maryland, Sch Med, Toxicol Program, Baltimore, MD 21201 USA. EM jeff.pettis@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 280-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800529 ER PT J AU Potter, T AF Potter, Thomas TI Interpreting kinetic data during adapted degradation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Potter, Thomas] USDA ARS, SE Watershed Res Lab, Tifton, GA 31794 USA. EM tom.potter@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 8-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800275 ER PT J AU Potter, T AF Potter, Thomas TI Interpreting kinetic data during adapted degradation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Potter, Thomas] USDA ARS, SE Watershed Res Lab, Tifton, GA 31794 USA. EM tom.potter@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 8-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800274 ER PT J AU Potter, TL Hapeman, CJ McConnell, LL Schmidt, WF Rice, CP Schaffer, BA AF Potter, Thomas L. Hapeman, Cathleen J. McConnell, Laura L. Schmidt, Walter F. Rice, Clifford P. Schaffer, Bruce A. TI Endosulfan wet depostion in southern Florida SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Potter, Thomas L.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. [Hapeman, Cathleen J.; McConnell, Laura L.; Schmidt, Walter F.; Rice, Clifford P.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Schaffer, Bruce A.] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. EM tom.potter@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 198-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800451 ER PT J AU Poulose, SM Bielinski, DF Gomes, SM Carrihill-Knoll, K Rabin, BM Shukitt-Hale, B AF Poulose, Shibu M. Bielinski, Donna F. Gomes, Stacey M. Carrihill-Knoll, Kirsty Rabin, Bernard M. Shukitt-Hale, Barbara TI Attenuation of stress/age associated toxic protein accrual and tau-hyperphosphorylation in brain by berries and walnuts SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Poulose, Shibu M.; Bielinski, Donna F.; Gomes, Stacey M.; Shukitt-Hale, Barbara] Tufts Univ, USDA, ARS, HNRCA, Boston, MA 01887 USA. [Carrihill-Knoll, Kirsty; Rabin, Bernard M.] Univ Maryland Baltimore Cty, Dept Psychol, Baltimore, MD 21250 USA. EM shibu.poulose@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 30-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800096 ER PT J AU Poulose, SM AF Poulose, Shibu M. TI Activation of neuronal housekeeping (autophagy) by polyphenol-rich berries and walnuts SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Poulose, Shibu M.] Tufts Univ, HNRCA, USDA ARS, Boston, MA 02111 USA. EM shibu.poulose@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 6-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800072 ER PT J AU Price, NPJ Faber, TA Hopkins, AC Vermillion, KE Fahey, GC AF Price, Neil P. J. Faber, Trevor A. Hopkins, Anne C. Vermillion, Karl E. Fahey, George C. TI Value-added prebiotic GGMO oligosaccharides from a high-volume molasses by-product of pine fiber board manufacture SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Price, Neil P. J.; Vermillion, Karl E.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Faber, Trevor A.; Fahey, George C.] Univ Illinois, Urbana, IL 61801 USA. [Hopkins, Anne C.] Temple Inland Inc, Appl Res Ctr, Diboll, TX 75941 USA. EM neil.price@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 42-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801415 ER PT J AU Qi, PX Nunez, A Wickham, ED AF Qi, Phoebe X. Nunez, Alberto Wickham, Edward D. TI Exploring reaction kinetics and mechanism between b-lactoglobulin and genipin SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Qi, Phoebe X.; Wickham, Edward D.] Agr Serv, USDA, Wyndmoor, PA 19038 USA. [Nunez, Alberto] Agr Serv, Off Ctr Director, USDA, Wyndmoor, PA USA. EM Phoebe.Qi@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 207-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800251 ER PT J AU Rice, CP Bialek-Kalinski, K McCarty, GW AF Rice, Clifford P. Bialek-Kalinski, Krystyna McCarty, Gregory W. TI Chiral separation of metolachlor ethane sulfonic acid as a groundwater dating tool SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Rice, Clifford P.; Bialek-Kalinski, Krystyna; McCarty, Gregory W.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM clifford.rice@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 35-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800299 ER PT J AU Rice, PJ Horgan, BP Rittenhouse, JL AF Rice, Pamela J. Horgan, Brian P. Rittenhouse, Jennifer L. TI Offsite transport of fungicides with snowmelt and rainfall runoff from golf course fairway turf SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Rice, Pamela J.; Rittenhouse, Jennifer L.] ARS, USDA, St Paul, MN 55108 USA. [Horgan, Brian P.] Univ Minnesota, Dept Hort, St Paul, MN 55108 USA. EM Pamela.Rice@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 285-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800534 ER PT J AU Rimando, AM Baerson, SR Pan, ZQ Polashock, JJ Dayan, FE Mizuno, CS Liu, CJ Snook, ME AF Rimando, Agnes M. Baerson, Scott R. Pan, Zhiqiang Polashock, James J. Dayan, Franck E. Mizuno, Cassia S. Liu, Chang-Jun Snook, Maurice E. TI Production of pterostilbene in Nicotiana species and Arabidopsis thaliana via co-expression of peanut stilbene synthase and sorghum O-methyltransferase SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Rimando, Agnes M.; Baerson, Scott R.; Pan, Zhiqiang; Dayan, Franck E.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS USA. [Polashock, James J.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Chatsworth, NJ USA. [Mizuno, Cassia S.] Univ New England, Dept Pharmaceut Sci, Portand, ME USA. [Liu, Chang-Jun] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Snook, Maurice E.] ARS, Toxicol & Mycotoxin Res Unit, USDA, Athens, GA USA. EM agnes.rimando@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 316-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800563 ER PT J AU Rotz, CA Hafner, SD AF Rotz, C. Alan Hafner, Sasha D. TI Predicting the reactive nitrogen footprint of milk production systems SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Rotz, C. Alan; Hafner, Sasha D.] USDA ARS, University Pk, PA 16802 USA. EM al.rotz@ars.usda.gov RI Hafner, Sasha/N-3970-2013 OI Hafner, Sasha/0000-0003-0955-0327 NR 0 TC 0 Z9 0 U1 1 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 159-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800413 ER PT J AU Samala, A Srinivasan, R Brooks, J AF Samala, Aditya Srinivasan, Radhakrishnan Brooks, John TI In vitro studies on xylo-oligosaccharides produced by autohydrolysis of corn fiber separated from DDGS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Samala, Aditya; Srinivasan, Radhakrishnan] Mississippi State Univ, Mississippi State, MS 39762 USA. [Brooks, John] USDA ARS, Mississippi State, MS 39762 USA. EM adityasamala3@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 43-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801416 ER PT J AU Sandahl, J AF Sandahl, Jason TI Global joint data generation project SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Sandahl, Jason] Foreign Agr Serv, USDA, Washington, DC 20250 USA. EM Jason.Sandahl@fas.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 54-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800316 ER PT J AU Sapozhnikova, Y AF Sapozhnikova, Yelena TI Multiclass, multiresidue method for analysis of pesticides, polychlorinated biphenyls, flame retardants and polyaromatic hydrocarbons in catfish using fast, low-pressure gas chromatography-tandem mass spectrometry SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Sapozhnikova, Yelena] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM yelena.sapozhnikova@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 27-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800292 ER PT J AU Senseman, S Camargo, E Krutz, J AF Senseman, Scott Camargo, Edinalvo Krutz, Jason TI Pesticide K-d values: How valuable are they? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Senseman, Scott; Camargo, Edinalvo] Texas A&M Univ, Dept Soil & Crop Sci, Texas AgriLife Res, College Stn, TX 77843 USA. [Camargo, Edinalvo] Conselho Nacl Desenvolvimento Cient & Tecnol, Brasilia, DF, Brazil. [Krutz, Jason] ARS, USDA, Stoneville, MS 38776 USA. EM s-senseman@tamu.edu RI Camargo, Edinalvo/I-6650-2013 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 1-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800268 ER PT J AU Shi, HM Xie, ZH Zhao, Y Chen, P Yu, LLL AF Shi, Haiming Xie, Zhuohong Zhao, Yang Chen, Pei Yu, Liangli L. TI LC-MS spectroscopic fingerprinting on analysis of diploid and tetraploid Gynostemma pentaphyllum SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Shi, Haiming] Shanghai Jiao Tong Univ, Dept Food Sci & Technol, Shanghai 200240, Peoples R China. [Xie, Zhuohong; Zhao, Yang; Yu, Liangli L.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Chen, Pei] USDA, Food Composit Lab, Beltsville, MD 20705 USA. EM hmshi@sjtu.edu.cn RI Xie, Zhuohong/P-3599-2014; shi, haiming/D-2806-2015 OI Xie, Zhuohong/0000-0001-8009-3523; NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 211-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800255 ER PT J AU Shortle, WC Smith, KT AF Shortle, Walter C. Smith, Kevin T. TI Role of forest fungi in biogeochemical cycling of root-available calcium in forest soils SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Shortle, Walter C.; Smith, Kevin T.] US Forest Serv, No Res Stn, USDA, Durham, NH 03824 USA. EM wshortle@fs.fed.us NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 18-GEOC PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804580 ER PT J AU Shrestha, B Payton, P Irin, F Green, M Canas, J AF Shrestha, Babina Payton, Paxton Irin, Fahmida Green, Micah Canas, Jaclyn TI Uptake and effects of multiwalled carbon nanotubes on germination and growth of various plant species SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Shrestha, Babina; Canas, Jaclyn] Texas Tech Univ, Dept Environm Toxicol, Lubbock, TX 79409 USA. [Payton, Paxton] USDA ARS, Plant Stress & Germplasm Dev Res Unit, Lubbock, TX 79415 USA. [Irin, Fahmida; Green, Micah] Texas Tech Univ, Dept Chem Engn, Lubbock, TX 79409 USA. EM babina.shrestha@tiehh.ttu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 319-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804524 ER PT J AU Silva, CJ Onisko, BC Dynin, I Erickson, ML Hui, C Carter, JM AF Silva, Christopher J. Onisko, Bruce C. Dynin, Irina Erickson, Melissa L. Hui, Colleen Carter, J. Mark TI Detecting and discriminating among pathogenic protein conformers (prions), using mass spectrometry-based and antibody-based approaches SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc, Amer Chem Soc, Div Chem Educ, Amer Chem Soc, Div Inorgan Chem, Soc Biol Inorgan Chem, Amer Chem Soc, Div Polymer Chem Inc C1 [Silva, Christopher J.; Dynin, Irina; Erickson, Melissa L.; Carter, J. Mark] ARS, WRRC, USDA, Albany, CA 94710 USA. [Onisko, Bruce C.] OniPro Biosci, Kensington, CA USA. [Hui, Colleen] Univ Calif Berkeley, Berkeley, CA 94720 USA. EM christopher.silva@ars.usda.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 216-ANYL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801086 ER PT J AU Silva, PJ AF Silva, Philip J. TI Adaptation of an ambient ion monitor for detection of amines in gas and particulate agricultural emissions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Silva, Philip J.] USDA ARS, Bowling Green, KY 42104 USA. EM phil.silva@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 255-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800504 ER PT J AU Solaimin, DKY Ashby, RD Zerkowski, JA AF Solaimin, Daniel K. Y. Ashby, Richard D. Zerkowski, Jonathan A. TI Sophorolipid biosurfactant synthesis and value-added derivatization SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Solaimin, Daniel K. Y.; Ashby, Richard D.; Zerkowski, Jonathan A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM dan.solaimin@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 300-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800547 ER PT J AU Sutivisedsak, N Leathers, TD Nunnally, MS Price, NPJ AF Sutivisedsak, Nongnuch Leathers, Timothy D. Nunnally, Melinda S. Price, Neil P. J. TI Production of the bioactive polysaccharide Schizophyllan from renewable cellulosic materials SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Sutivisedsak, Nongnuch; Leathers, Timothy D.; Nunnally, Melinda S.; Price, Neil P. J.] ARS, USDA, Peoria, IL 61604 USA. EM nuch.sutivisedsak@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 14-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801391 ER PT J AU Swale, DR Carlier, PR Hartsel, JA Totrov, MM Temeyer, KB Bloomquist, JR AF Swale, Daniel R. Carlier, Paul R. Hartsel, Joshua A. Totrov, Maxim M. Temeyer, Kevin B. Bloomquist, Jeffrey R. TI Design of novel carbamate insecticides with superior selectivity for the control of cattle tick (Boophilus microplus) and sandfly (Phlebotomus papatasi) populations SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Swale, Daniel R.; Bloomquist, Jeffrey R.] Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32608 USA. [Carlier, Paul R.; Hartsel, Joshua A.] Virginia Polytech Inst & State Univ, Dept Chem, Blacksburg, VA 24061 USA. [Totrov, Maxim M.] Molsoft LLC, La Jolla, CA 92307 USA. [Temeyer, Kevin B.] ARS, Knipling Bushland US Livestock Insects Res Lab, USDA, Kerrville, TX 78028 USA. EM dswale@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 102-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800363 ER PT J AU Taylor, MM Medina, MB Brown, EM Liu, CK AF Taylor, Maryann M. Medina, Marjorie B. Brown, Eleanor M. Liu, C-K TI Preparation, characterization, and utilization of polyphenol-modified gelatin products SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Taylor, Maryann M.; Medina, Marjorie B.; Brown, Eleanor M.; Liu, C-K] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA. EM maryann.taylor@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 134-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800190 ER PT J AU Temeyer, KB Olafson, PU Brake, DK Tuckow, AP Li, AY de Leon, AAP AF Temeyer, Kevin B. Olafson, Pia U. Brake, Danett K. Tuckow, Alexander P. Li, Andrew Y. de Leon, Adalberto A. Perez TI Acetylcholinesterases of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Temeyer, Kevin B.; Olafson, Pia U.; Brake, Danett K.; Tuckow, Alexander P.; Li, Andrew Y.; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. EM kevin.temeyer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 124-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800379 ER PT J AU Todd, RW Cole, NA AF Todd, Richard W. Cole, N. Andy TI Nitrogen enrichment and species change in shortgrass prairie adjacent to a cattle feedyard SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Todd, Richard W.; Cole, N. Andy] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. EM richard.todd@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 218-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800468 ER PT J AU Todd, RW Cole, NA AF Todd, Richard W. Cole, N. Andy TI Ammonia emissions from beef cattle feedyards: A summary of seven years of research SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Todd, Richard W.; Cole, N. Andy] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. EM richard.todd@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 161-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800415 ER PT J AU Trabue, SL Kerr, BJ AF Trabue, Steven L. Kerr, Brian J. TI Impact of animal diet on ammonia and greenhouse gas emissions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Trabue, Steven L.; Kerr, Brian J.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50014 USA. EM steven.trabue@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 254-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800503 ER PT J AU Truman, C Potter, T AF Truman, Clint Potter, Tom TI How constant are pesticide K-d values during a rainfall event? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Truman, Clint; Potter, Tom] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. EM clint.truman@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 4-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800271 ER PT J AU Tunick, MH Onwulata, CI AF Tunick, Michael H. Onwulata, Charles I. TI Using temperature sweeps to investigate rheology of bioplastics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Tunick, Michael H.; Onwulata, Charles I.] ARS, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, USDA, Wyndmoor, PA 19038 USA. EM michael.tunick@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 196-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800240 ER PT J AU Uchimiya, M AF Uchimiya, Minori TI Roles of black carbon on the fate of heavy metals and agrochemicals in soil SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Uchimiya, Minori] ARS, Commod Utilizat Res Unit, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 8-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804240 ER PT J AU Uchimiya, M AF Uchimiya, Minori TI Sorption of organophosphate and triazine agrochemicals on biochars and soils SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Uchimiya, Minori] USDA ARS, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 229-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800479 ER PT J AU Ukuku, D Yamamoto, K Kawamoto, S AF Ukuku, Dike Yamamoto, Kazutaka Kawamoto, Shinichi TI Cellular damage of Escherichia coli O157:H7 and Salmonella spp. in apple juice treated with high hydrostatic pressure and thermal death time disks SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Ukuku, Dike] ARS, FSIT, USDA, Wyndmoor, PA 19038 USA. [Yamamoto, Kazutaka; Kawamoto, Shinichi] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan. EM dike.ukuku@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 36-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800102 ER PT J AU Van Hekken, DL Iandola, SK Tunick, MH AF Van Hekken, Diane L. Iandola, Susan K. Tunick, Michael H. TI Volatile compounds in milled/no-mill Queso Fresco during storage SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Van Hekken, Diane L.; Iandola, Susan K.; Tunick, Michael H.] ARS, Dairy & Funct Foods Res Unit, USDA, Wyndmoor, PA 19038 USA. EM diane.vanhekken@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 151-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800205 ER PT J AU Waldrip, HM Todd, RW Cole, NA Li, CS Salas, W AF Waldrip, Heidi M. Todd, Richard W. Cole, N. Andy Li, Changsheng Salas, William TI Estimation of ammonia emissions from beef cattle feedyards in the southern High Plains with process-based modeling SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Waldrip, Heidi M.; Todd, Richard W.; Cole, N. Andy] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Li, Changsheng] Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. [Salas, William] Appl Geosolut LLC, Durham, NH 03824 USA. EM heidi.waldrip@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 160-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800414 ER PT J AU Whigham, LD AF Whigham, Leah D. TI CLA in obesity SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Whigham, Leah D.] USDA ARS, Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM Leah.Whigham@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 110-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800170 ER PT J AU Wong, DWS Chan, VJ McCormack, AA AF Wong, Dominic W. S. Chan, Victor J. McCormack, Amanda A. TI Gene cloning and characterization of a bifunctional endo-1,4-b-mannanase/1,3-1,4-b-glucanase enzyme and its individual domains SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Wong, Dominic W. S.; Chan, Victor J.; McCormack, Amanda A.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. EM Dominic.Wong@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 130-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800696 ER PT J AU Wood, DF Williams, TG Offeman, RD Mann, P Orts, WJ AF Wood, Delilah F. Williams, Tina G. Offeman, Richard D. Mann, Paul Orts, William J. TI Focus on agricultural residues: Microstructure of almond hull SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Wood, Delilah F.; Williams, Tina G.; Offeman, Richard D.; Orts, William J.] ARS, Bioprod Chem & Engn Res Unit, USDA, WRRC, Albany, CA 94710 USA. [Mann, Paul] Bio Fuels & Mass Consulting, Caldwell, ID 83605 USA. EM de.wood@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 194-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800238 ER PT J AU Xu, JY Liu, ZS Kim, S Liu, SX AF Xu, Jingyuan Liu, Zengshe Kim, Sanghoon Liu, Sean X. TI Rheological properties of a biological thermo-responsive hydrogel produced from soybean oil polymers SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Xu, Jingyuan; Liu, Zengshe; Kim, Sanghoon; Liu, Sean X.] ARS, Natl Ctr Agr Utilizat Rearch, USDA, Peoria, IL 61604 USA. EM james.xu@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 285-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621808047 ER PT J AU Xuan, RC Ashworth, DJ Yates, SR AF Xuan, Richeng Ashworth, Daniel J. Yates, Scott R. TI Depleting methyl iodide emissions from fumigated soils SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Xuan, Richeng; Ashworth, Daniel J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Xuan, Richeng; Ashworth, Daniel J.; Yates, Scott R.] USDA ARS, US Salin Lab, Contaminant Fate & Transport Res Unit, Riverside, CA 92507 USA. EM richeng.xuan@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 87-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800348 ER PT J AU Yandeau-Nelson, MD Condon, S Jose, A Peddicord, L Lopez, M Qin, WM Lauter, N Nikolau, BJ AF Yandeau-Nelson, Marna D. Condon, Samson Jose, Adarsh Peddicord, Layton Lopez, Miriam Qin, Wenmin Lauter, Nick Nikolau, Basil J. TI Dissecting the metabolic network associated with hydrocarbon synthesis using maize silks as a model system SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Yandeau-Nelson, Marna D.; Condon, Samson; Jose, Adarsh; Qin, Wenmin; Nikolau, Basil J.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Lopez, Miriam; Lauter, Nick] Iowa State Univ, USDA ARS Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Peddicord, Layton; Lauter, Nick] Iowa State Univ, Interdept Genet Grad Program, Ames, IA 50011 USA. EM myn@iastate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 12-IEC PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621804647 ER PT J AU Yates, SR Gish, T Luo, LF AF Yates, Scott R. Gish, Timothy Luo, Lifang TI Modeling pesticide volatilization for complex soil and environmental conditions SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Yates, Scott R.; Luo, Lifang] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Gish, Timothy] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM scott.yates@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 55-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800317 ER PT J AU Youngblood, JP Moon, RJ Liu, JC Diaz, AJ Riesing, A AF Youngblood, Jeffrey P. Moon, Robert J. Liu, Jen-Chieh Diaz A, Jairo A. Riesing, Alexander TI Thermal and mechanical properties of cellulose nanocrystal and nanofibril composites SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Youngblood, Jeffrey P.; Moon, Robert J.; Liu, Jen-Chieh; Diaz A, Jairo A.; Riesing, Alexander] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. EM jpyoungb@purdue.edu NR 0 TC 0 Z9 0 U1 1 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 61-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621801433 ER PT J AU Zhu, HP AF Zhu, Heping TI Development of intelligent spraying systems to reduce airborne spray drift SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Zhu, Heping] ARS, Applicat Technol Res Unit, USDA, Wooster, OH 44691 USA. EM heping.zhu@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 56-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800318 ER PT J AU Zou, YH Zheng, W Bradford, S AF Zou, Yonghong Zheng, Wei Bradford, Scott TI Colloid facilitated transport of veterinary pharmaceutical florfenicol in saturated sand and soil columns SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 244th National Fall Meeting of the American-Chemical-Society (ACS) CY AUG 19-23, 2012 CL Philadelphia, PA SP Amer Chem Soc, Div Hist Chem, Amer Chem Soc C1 [Zou, Yonghong; Zheng, Wei] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Bradford, Scott] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. EM yhzou@illinois.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 19 PY 2012 VL 244 MA 228-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 220XX UT WOS:000324621800478 ER PT J AU Anderson, RG Lo, MH Famiglietti, JS AF Anderson, Ray G. Lo, Min-Hui Famiglietti, James S. TI Assessing surface water consumption using remotely-sensed groundwater, evapotranspiration, and precipitation SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article ID SATELLITE IMAGERY; GRAVITY RECOVERY; CLIMATE; FIELD; IRRIGATION; RESOURCES; MISSION; MODEL; GRACE AB Estimates of consumptive use of surface water by agriculture are vital for assessing food security, managing water rights, and evaluating anthropogenic impacts on regional hydrology. However, reliable, current, and public data on consumptive use can be difficult to obtain, particularly in international and less developed basins. We combine remotely-sensed precipitation and satellite observations of evapotranspiration and groundwater depletion to estimate surface water consumption by irrigated agriculture in California's Central Valley for the 2004-09 water years. We validated our technique against measured consumption data determined from streamflow observations and water export data in the Central Valley. Mean satellite-derived surface water consumption was 291.0 +/- 32.4 mm/year while measured surface water consumption was 308.1 +/- 6.5 mm/year. The results show the potential for remotely- sensed hydrologic data to independently observe irrigated agriculture's surface water consumption in contested or unmonitored basins. Improvements in the precision and spatial resolution of satellite precipitation, evapotranspiration and gravimetric groundwater observations are needed to reduce the uncertainty in this method and to allow its use on smaller basins and at shorter time scales. Citation: Anderson, R. G., M.-H. Lo, and J. S. Famiglietti (2012), Assessing surface water consumption using remotely-sensed groundwater, evapotranspiration, and precipitation, Geophys. Res. Lett., 39, L16401, doi:10.1029/2012GL052400. C1 [Anderson, Ray G.; Lo, Min-Hui; Famiglietti, James S.] Univ Calif Irvine, UC Ctr Hydrol Modeling, Irvine, CA USA. [Famiglietti, James S.] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA USA. [Famiglietti, James S.] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA USA. RP Anderson, RG (reprint author), USDA ARS, Water Management Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM ray.anderson@ars.usda.gov OI LO, MIN-HUI/0000-0002-8653-143X; Anderson, Ray/0000-0002-6202-5890 FU NASA's GRACE science team; University of California's Multicampus Research Programs and Initiatives FX Funding was provided by grants from NASA's GRACE science team and the University of California's Multicampus Research Programs and Initiatives. We thank Qiuhong Tang at the Chinese Academy of Sciences/University of Washington for providing evapotranspiration data. NR 35 TC 11 Z9 11 U1 2 U2 49 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD AUG 18 PY 2012 VL 39 AR L16401 DI 10.1029/2012GL052400 PG 6 WC Geosciences, Multidisciplinary SC Geology GA 991ZM UT WOS:000307743300001 ER PT J AU Jia, YL Jia, MH Wang, XY Liu, GJ AF Jia, Yulin Jia, Melissa H. Wang, Xueyan Liu, Guangjie TI Indica and Japonica Crosses Resulting in Linkage Block and Recombination Suppression on Rice Chromosome 12 SO PLOS ONE LA English DT Article ID BLAST RESISTANCE GENE; PI-TA; ORYZA-SATIVA; WILD-RICE; MOLECULAR CHARACTERIZATION; MAPPING POPULATION; CULTIVATED RICE; REGISTRATION; MARKERS; LOCUS AB Understanding linkage block size and molecular mechanisms of recombination suppression is important for plant breeding. Previously large linkage blocks ranging from 14 megabases to 27 megabases were observed around the rice blast resistance gene Pi-ta in rice cultivars and backcross progeny involving an indica and japonica cross. In the present study, the same linkage block was further examined in 456 random recombinant individuals of rice involving 5 crosses ranging from F-2 to F-10 generation, with and without Pi-ta containing genomic indica regions with both indica and japonica germplasm. Simple sequence repeat markers spanning the entire chromosome 12 were used to detect recombination break points and to delimit physical size of linkage blocks. Large linkage blocks ranging from 4.1 megabases to 10 megabases were predicted from recombinant individuals involving genomic regions of indica and japonica. However, a significantly reduced block from less than 800 kb to 2.1megabases was identified from crosses of indica with indica rice regardless of the existence of Pi-ta. These findings suggest that crosses of indica and japonica rice have significant recombination suppression near the centromere on chromosome 12. C1 [Jia, Yulin; Jia, Melissa H.] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR USA. [Wang, Xueyan] China Jiliang Univ, Coll Life Sci, Dept Biol, Hangzhou, Zhejiang, Peoples R China. [Liu, Guangjie] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR USA. RP Jia, YL (reprint author), ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR USA. EM Yulin.jia@ars.usda.gov FU National Natural Science Foundation of China [31000847]; Science and Technology Department of Zhejiang Province [2011R10038] FX Financial supports of National Natural Science Foundation of China (Grant No. 31000847) and of Science and Technology Department of Zhejiang Province (Grant No. 2011R10038) to XW are also acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 8 Z9 9 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 17 PY 2012 VL 7 IS 8 AR e43066 DI 10.1371/journal.pone.0043066 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 996DG UT WOS:000308063700035 PM 22912788 ER PT J AU Luo, YG Nou, XW Millner, P Zhou, B Shen, CL Yang, Y Wu, YP Wang, Q Feng, H Shelton, D AF Luo, Yaguang Nou, Xiangwu Millner, Patricia Zhou, Bin Shen, Cangliang Yang, Yang Wu, Yunpeng Wang, Qin Feng, Hao Shelton, Dan TI A pilot plant scale evaluation of a new process aid for enhancing chlorine efficacy against pathogen survival and cross-contamination during produce wash SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE E. coli O157:H7; Fresh-cut produce wash; Lettuce; Organic load ID ESCHERICHIA-COLI O157; WATER-QUALITY; LETTUCE; H7 AB Developing food safety intervention technology that can be readily adopted by the industry often requires test conditions that match as closely as possible to those of commercial food processing operations; yet biosafety risks inherent in pathogen studies constrain most experiments to laboratory settings. In this study, we report the first semi-commercial pilot-scale evaluation of a new process aid, T128, for its impact on enhancing the antimicrobial efficacy of chlorinated wash water against pathogen survival and cross-contamination. A non-pathogenic, BSL-1, strain of Escherichia coli O157:H7 was inoculated onto freshly harvested baby spinach leaves and washed with large amounts of freshly cut un-inoculated iceberg lettuce shreds in wash water with free chlorine periodically replenished, in the presence or absence of T128. Changes in water quality and pathogen survival and cross-contamination were monitored at every 2 min intervals for up to 36 min for each treatment during the wash operation. Results indicated that the use of T128 did not significantly (P > 0.05) influence the rate of wash water deterioration, nor the pathogen populations remaining on the inoculated spinach leaves. However, in the absence of T128 (control), survival of E. coli O157:H7 in wash water and cross-contamination of un-inoculated lettuce frequently occurred when free chlorine in solution dropped below 1 mg/l during the wash process. In contrast:, the use of T128 significantly reduced the occurrence of E. coli O157:H7 surviving in wash water and of cross-contamination to un-inoculated shredded iceberg lettuce under the same operational conditions, suggesting that the application of T128 in a chlorine-based fresh produce sanitization system could increase the safety margin of process control on fresh-cut operations. Published by Elsevier B.V. C1 [Luo, Yaguang; Nou, Xiangwu; Millner, Patricia; Zhou, Bin; Shen, Cangliang; Yang, Yang; Shelton, Dan] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Wu, Yunpeng; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. [Feng, Hao] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. RP Luo, YG (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov FU Center for Produce Safety (CPS), University of California, Davis, CA, USA [2009-74] FX The authors wish to thank Jim Brennan, Danny Lindstrom, Tanya Mason, and the entire pilot plant staff at New Leaf Food Safety Solutions LLC (Salinas, CA, USA) for providing strong technical support during our 4-week long pilot plant trials. We also want to thank Dr. David Ingram for making the schematic drawing of the pilot plant flow diagram, and Ellen Turner for sample preparation and analysis. This project is partially supported by grant number 2009-74 from Center for Produce Safety (CPS), University of California, Davis, CA, USA. Use of a company name or product by the USDA does not imply approval or recommendation of the product to the exclusion of others that also may be suitable. NR 20 TC 30 Z9 31 U1 2 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD AUG 17 PY 2012 VL 158 IS 2 BP 133 EP 139 DI 10.1016/j.ijfoodmicro.2012.07.008 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 002HR UT WOS:000308523200007 PM 22857846 ER PT J AU Colaianni, G Sun, L Di Benedetto, A Tamma, R Zhu, LL Cao, J Grano, M Yuen, T Colucci, S Cuscito, C Mancini, L Li, JH Nishimori, K Bab, I Lee, HJ Iqbal, J Young, WS Rosen, C Zallone, A Zaidi, M AF Colaianni, Graziana Sun, Li Di Benedetto, Adriana Tamma, Roberto Zhu, Ling-Ling Cao, Jay Grano, Maria Yuen, Tony Colucci, Sylvia Cuscito, Concetta Mancini, Lucia Li, Jianhua Nishimori, Katsuhiko Bab, Itai Lee, Heon-Jin Iqbal, Jameel Young, W. Scott, III Rosen, Clifford Zallone, Alberta Zaidi, Mone TI Bone Marrow Oxytocin Mediates the Anabolic Action of Estrogen on the Skeleton SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID RECEPTOR; EXPRESSION; HORMONE; MICE; LACTATION; GENE; DIFFERENTIATION; PARTURITION; CELLS; ACTH AB Estrogen uses two mechanisms to exert its effect on the skeleton: it inhibits bone resorption by osteoclasts and, at higher doses, can stimulate bone formation. Although the antiresorptive action of estrogen arises from the inhibition of the MAPK JNK, the mechanism of its effect on the osteoblast remains unclear. Here, we report that the anabolic action of estrogen in mice occurs, at least in part, through oxytocin (OT) produced by osteoblasts in bone marrow. We show that the absence of OT receptors (OTRs) in OTR-/- osteoblasts or attenuation of OTR expression in silenced cells inhibits estrogen-induced osteoblast differentiation, transcription factor up-regulation, and/or OT production in vitro. In vivo, OTR-/- mice, known to have a bone formation defect, fail to display increases in trabecular bone volume, cortical thickness, and bone formation in response to estrogen. Furthermore, osteoblast-specific Col2.3-Cre(+)/OTRfl/fl mice, but not TRAP-Cre(+)/OTRfl/fl mice, mimic the OTR-/- phenotype and also fail to respond to estrogen. These data attribute the phenotype of OTR deficiency to an osteoblastic rather than an osteoclastic defect. Physiologically, feed-forward OT release in bone marrow by a rising estrogen concentration may facilitate rapid skeletal recovery during the latter phases of lactation. C1 [Colaianni, Graziana; Di Benedetto, Adriana; Tamma, Roberto; Grano, Maria; Colucci, Sylvia; Cuscito, Concetta; Mancini, Lucia; Zallone, Alberta] Univ Bari, Sch Med, Dept Human Anat & Histol, I-70124 Bari, Italy. [Colaianni, Graziana; Sun, Li; Zhu, Ling-Ling; Yuen, Tony; Li, Jianhua; Iqbal, Jameel; Zaidi, Mone] Mt Sinai Sch Med, Mt Sinai Bone Program, New York, NY 10029 USA. [Cao, Jay] Grand Forks Human Nutr Res Ctr, ARS, USDA, Grand Forks, ND 58201 USA. [Nishimori, Katsuhiko] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan. [Bab, Itai] Hebrew Univ Jerusalem, Bone Lab, IL-91120 Jerusalem, Israel. [Lee, Heon-Jin] Kyungpook Natl Univ, Dept Oral Microbiol, Sch Dent, Taegu 702701, South Korea. [Young, W. Scott, III] NIMH, Sect Neural Gene Express, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Rosen, Clifford] Maine Med Ctr, Res Inst, Scarborough, ME 04074 USA. RP Zallone, A (reprint author), Univ Bari, Sch Med, Dept Human Anat & Histol, I-70124 Bari, Italy. EM alberta.zallone@fastwebnet.it; mone.zaidi@mssm.edu RI Young, W Scott/A-9333-2009; tamma, roberto/C-3679-2016; OI Young, W Scott/0000-0001-6614-5112; Colaianni, Graziana/0000-0001-8501-5399; Grano, Maria/0000-0002-7121-5899 FU National Institutes of Health from NIA [AG023176, AG040132]; NIDDK [DK080459]; National Institute of Mental Health [Z01-MH-002498-23]; Italian Space Agency; European Space Agency; Ministero dell'Istruzione, dell'Universita della Ricerca; United States Department of Agriculture Agricultural Research Service [5450-51000-046-00D]; American Federation for Aging Research FX This work was supported, in whole or in part, by National Institutes of Health Grants AG023176 and AG040132 from NIA (to M. Z.) and DK080459 from NIDDK (to M. Z. and L. S.) and by National Institute of Mental Health Intramural Research Program Grant Z01-MH-002498-23 (to H.-J. L. and W. S. Y.). This work was also supported by grants from the Italian Space Agency (Osteoporosis and Muscular Atrophy Project), the European Space Agency (European Research in Space and Terrestrial Osteoporosis Microgravity Application Promotion Project), and the Ministero dell'Istruzione, dell'Universita della Ricerca (to A. Z.); by United States Department of Agriculture Agricultural Research Service Current Research Information System Program Grant 5450-51000-046-00D (to J. C.); and by the American Federation for Aging Research (to J. I.). NR 33 TC 25 Z9 27 U1 1 U2 6 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 17 PY 2012 VL 287 IS 34 BP 29159 EP 29167 DI 10.1074/jbc.M112.365049 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 996HD UT WOS:000308074600081 PM 22761429 ER PT J AU Miller, R Estrada-Pena, A Almazan, C Allen, A Jory, L Yeater, K Messenger, M Ellis, D de Leon, AAP AF Miller, Robert Estrada-Pena, Agustin Almazan, Consuelo Allen, Andrew Jory, Lauren Yeater, Kathleen Messenger, Matthew Ellis, Dee de Leon, Adalberto A. Perez TI Exploring the use of an anti-tick vaccine as a tool for the integrated eradication of the cattle fever tick, Rhipicephalus (Boophilus) annulatus SO VACCINE LA English DT Article DE Boophilus; Tick; Vaccine; Modeling; Bm86 ID OVIPOSITIONAL BIOLOGY; TEXAS/MEXICO BORDER; MICROPLUS ACARI; INFESTATIONS; IXODIDAE; MEXICO; TEXAS AB Bovine babesiosis, also known as cattle fever, is a tick-borne protozoal disease foreign to the United States. It was eradicated by eliminating the vector species, Rhipicephalus (Boophilus) annulatus and Rhipicephalus (Boophilus) microplus, through the efforts of the Cattle Fever Tick Eradication Program (CFTEP), with the exception of a permanent quarantine zone (PQZ) in south Texas along the border with Mexico. Keeping the U.S. free of cattle fever ticks in a sustainable manner is a critical national agricultural biosecurity issue. The efficacy of a Bm86-based anti-tick vaccine commercialized outside of the U.S. was evaluated against a strain of R. annulatus originated from an outbreak in Texas. Vaccination controlled 99.9 and 91.4% of the ticks 8 weeks and 5.5 months after the initial vaccination, respectively. Computer modeling of habitat suitability within the PQZ typically at risk of re-infestation with R. annulatus from Mexico predicted that at a level of control greater than 40%, eradication would be maintained indefinitely. Efficacy and computer modeling data indicate that the integration of vaccination using a Bm86-based anti-tick vaccine with standard eradication practices within the northwestern half of the PQZ could incentivize producers to maintain cattle on pasture thereby avoiding the need to vacate infested premises. Implementing this epidemiologically proactive strategy offers the opportunity to prevent R. annulatus outbreaks in the U.S., which would represent a significant shift in the way the CFTEP operates. (C) 2012 Published by Elsevier Ltd. C1 [Miller, Robert] ARS, USDA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. [Estrada-Pena, Agustin] Univ Zaragoza, Fac Med Vet, Zaragoza 50013, Spain. [Almazan, Consuelo] Univ Autonoma Tamaulipas, Fac Med Vet & Zootecnia, Ciudad Victoria 87000, Tamaulipas, Mexico. [Allen, Andrew; Jory, Lauren] Pfizer Australia, Vet Med Res & Dev, Parkville, Vic 3052, Australia. [Yeater, Kathleen] ARS, USDA, So Plains Area, College Stn, TX 77840 USA. [Messenger, Matthew] USDA, Cattle Fever Tick Eradicat Program, APHIS, VS, Riverdale, MD 20737 USA. [Ellis, Dee] Texas Anim Hlth Commiss, Austin, TX 78758 USA. [de Leon, Adalberto A. Perez] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Miller, R (reprint author), ARS, USDA, Cattle Fever Tick Res Lab, 22675 N Moorefield Rd,Bldg 6419, Edinburg, TX 78541 USA. EM robert.miller@ars.usda.gov OI Estrada Pena, Agustin/0000-0001-7483-046X FU USDA-APHIS Cattle Fever Tick Eradication Program; Texas Animal Health Commission FX The authors thank Drs. Rodrigo Rosario-Cruz and Felix Guerrero for critical review of this manuscript, Dr. Peter Willadsen for critical guidance, Mr. Krska, Moses, and Tidwell for technical support, and the members of the USDA-APHIS-VS, and the USDA-ARS-CFTRL who made this research possible. This work was funded by the USDA-APHIS Cattle Fever Tick Eradication Program and Texas Animal Health Commission. This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation of endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 20 TC 8 Z9 9 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD AUG 17 PY 2012 VL 30 IS 38 BP 5682 EP 5687 DI 10.1016/j.vaccine.2012.05.061 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 000JF UT WOS:000308381900020 PM 22687762 ER PT J AU Erol, E Sells, SF Williams, NM Kennedy, L Locke, SJ Labeda, DP Donahue, JM Carter, CN AF Erol, Erdal Sells, Stephen F. Williams, Neil M. Kennedy, Laura Locke, Stephen J. Labeda, David P. Donahue, James M. Carter, Craig N. TI An investigation of a recent outbreak of nocardioform placentitis caused abortions in horses SO VETERINARY MICROBIOLOGY LA English DT Article DE Nocardioform placentitis; Actinomycetes; Horse; PCR; Sequencing; Phylogenetic analysis ID SP NOV.; EQUINE PLACENTAS; CROSSIELLA-EQUI; AMYCOLATOPSIS; INFECTION AB Nocardioform placentitis associated with gram positive branching actinomycetes caused a record number of abortions in mares diagnosed by the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) affecting the 2011 foal crop (2011 foal crop: the cohort of foals conceived during the 2010 breeding season). The goal of the present study is to make a comprehensive analysis of this outbreak in terms of frequencies of the bacteria causing nocardioform placentitis mediated abortions and to investigate the ages of fetuses, abortion months and breeding times. In the present study, characteristic slow-growing, pungent/soil odor gram positive branching actinomycetes were recovered in high numbers in placental specimens in 76 abortion cases diagnosed as nocardioform placentitis by pathologists. To determine the type of actinomycetes responsible for the abortions, PCR assays were performed on the gram positive branching bacilli. The most prominent actinomycetes species were Amycolatopsis spp. (37 cases, 48.7%) and Crossiella equi (C. equi) (22 cases, 28.9%). Six cases (7.9%) contained both Amycolatopsis spp., and C. equi. 10 isolates were unidentified by PCR assays and shown to have high DNA sequence homology to Streptomyces species, Microbacterium species, Nocardia species and Allokutzneria species, as evidenced by 16 rRNA gene sequencing and phylogenetic analysis. Nocardioform placentitis related abortions occurred mostly between December 2010 and April 2011 happening exclusively in the last trimester. Breeding time of aborted pregnancies ranged from March 2010 to July 2010, suggesting that if transmission of the actinomycetes agents occurred during breeding, it was not related to a specific season. Published by Elsevier B.V. C1 [Erol, Erdal; Sells, Stephen F.; Williams, Neil M.; Kennedy, Laura; Locke, Stephen J.; Donahue, James M.; Carter, Craig N.] Univ Kentucky, Coll Agr & Publ Hlth, Vet Diagnost Lab, Lexington, KY 40512 USA. [Labeda, David P.] USDA ARS, Microbial Genom & Bioproc Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Erol, E (reprint author), Univ Kentucky, Coll Agr & Publ Hlth, Vet Diagnost Lab, Lexington, KY 40512 USA. EM erdal.erol@uky.edu NR 23 TC 6 Z9 6 U1 0 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 J9 VET MICROBIOL JI Vet. Microbiol. PD AUG 17 PY 2012 VL 158 IS 3-4 BP 425 EP 430 DI 10.1016/j.vetmic.2012.02.023 PG 6 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 986FN UT WOS:000307326800026 PM 22410309 ER PT J AU Xue, YW Gao, B Yao, Y Inyang, M Zhang, M Zimmerman, AR Ro, KS AF Xue, Yingwen Gao, Bin Yao, Ying Inyang, Mandu Zhang, Ming Zimmerman, Andrew R. Ro, Kyoung S. TI Hydrogen peroxide modification enhances the ability of biochar (hydrochar) produced from hydrothermal carbonization of peanut hull to remove aqueous heavy metals: Batch and column tests SO CHEMICAL ENGINEERING JOURNAL LA English DT Article DE Hydrothermally produced biochar; Hydrogen peroxide; Filtration; Metal/risk element ID MULTIWALLED CARBON NANOTUBES; DIGESTED SUGARCANE BAGASSE; STRUCTURAL-PROPERTIES; BEET TAILINGS; ADSORPTION; SORPTION; BIOMASS; LEAD; CADMIUM; MECHANISMS AB Biochar converted from agricultural residues can be used as an alternative adsorbent for removal of aqueous heavy metals. In this work, experimental and modeling investigations were conducted to examine the effect of H2O2 treatment on hydrothermally produced biochar (hydrochar) from peanut hull to remove aqueous heavy metals. Characterization measurements showed that H2O2 modification increased the oxygen-containing functional groups, particularly carboxyl groups, on the hydrochar surfaces. As a result, the modified hydrochar showed enhanced lead sorption ability with a sorption capacity of 22.82 mg g(-1), which was comparable to that of commercial activated carbon and was more than 20 times of that of untreated hydrochar (0.88 mg g(-1)). When used as filter media in a packed column, the modified hydrochar was also much more effective in filtering lead than the unmodified one. The lead removal capacity of the modified hydrochar packed column was about 20 times of that containing untreated hydrochar. In a multi-metal system, the modified hydrochar column still effectively removed lead, as well as other heavy metals (i.e., Cu2+, Ni2+, and Cd2+) from water flow. Model results indicated that the heavy metal removal ability of the modified hydrochar follows the order of Pb2+ > Cu2+ > Cd2+ > Ni2+. Findings from this work suggest that H2O2-modified hydrochar may be an effective, less costly, and environmentally sustainable adsorbent for many environmental applications, particularly with respect to metal immobilization. (C) 2012 Elsevier B.V. All rights reserved. C1 [Xue, Yingwen; Gao, Bin; Yao, Ying; Inyang, Mandu; Zhang, Ming] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Xue, Yingwen] Wuhan Univ, Sch Civil Engn, Wuhan 430072, Hubei, Peoples R China. [Zimmerman, Andrew R.] Univ Florida, Dept Geol Sci, Gainesville, FL 32611 USA. [Ro, Kyoung S.] USDA, ARS Coastal Plains Soil, Water & Plant Res Ctr, Florence, SC 29501 USA. RP Gao, B (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM bg55@ufl.edu RI Gao, Bin/B-8027-2010; Xue, Yingwen/M-1075-2016; OI Gao, Bin/0000-0003-3769-0191; Zimmerman, Andrew/0000-0001-5137-4916 FU NSF [CBET-1054405]; USDA [58-3148-1-179, T-STAR-2009-34135-20192]; Florida Peanut Check-off program FX This research was partially supported by the NSF (CBET-1054405), the USDA (58-3148-1-179 and T-STAR-2009-34135-20192), and the Florida Peanut Check-off program. The authors also thank the anonymous reviewers for their invaluable insight and helpful suggestions. NR 38 TC 82 Z9 86 U1 40 U2 303 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 1385-8947 J9 CHEM ENG J JI Chem. Eng. J. PD AUG 15 PY 2012 VL 200 BP 673 EP 680 DI 10.1016/j.cej.2012.06.116 PG 8 WC Engineering, Environmental; Engineering, Chemical SC Engineering GA 017UF UT WOS:000309615800074 ER PT J AU Penn, CA Wemple, BC Campbell, JL AF Penn, Colin A. Wemple, Beverley C. Campbell, John L. TI Forest influences on snow accumulation and snowmelt at the Hubbard Brook Experimental Forest, New Hampshire, USA SO HYDROLOGICAL PROCESSES LA English DT Article; Proceedings Paper CT Eastern Snow Conference (ESC) CY 2011 CL Montreal, CANADA DE snow distribution; snow water equivalent (SWE); snowmelt; forests; sky view factor ID RAIN-ON-SNOW; CLEAR-CUT; BRITISH-COLUMBIA; TIMBER HARVEST; COVER; MELT; RADIATION; CANOPIES; PATTERNS; ABLATION AB Many factors influence snow depth, water content and duration in forest ecosystems. The effects of forest cover and canopy gap geometry on snow accumulation has been well documented in coniferous forests of western North America and other regions; however, few studies have evaluated these effects on snowpack dynamics in mixed deciduous forests of the northeastern USA. We measured snow depth and water equivalent near the time of peak snowpack accumulation and, again, during snowmelt to better understand the effect of forests on snowpack properties in the northeastern USA. Surveys occurred in openings and under the forest canopy at plots with different characteristics (e.g. aspect, elevation, forest composition) within the Hubbard Brook Experimental Forest in New Hampshire, USA. Snow water equivalent (SWE) was significantly greater in openings (?p?=?0.021) than in forests on north-facing plots but not on south-facing plots (?p?=?0.318) in early March 2009. One month later, SWE was more variable but remained greater in openings on north-facing plots (?p?=?0.067), whereas SWE was greater (?p?=?0.071) under forests than in clearings on south-facing plots, where snowmelt had sufficiently progressed. During peak accumulation, SWE decreased with increasing conifer cover on north-facing plots. During the snowmelt period, SWE on south-facing plots decreased with increasing basal area, sky view factor and diameter at breast height of trees on the plots. These results have implications for spring streamflow and soil moisture in the face of changing climate conditions and land use pressures in the forests of northern New England. Published 2012. This article is a U.S. Government work and is in the public domain in the USA. C1 [Wemple, Beverley C.] Univ Vermont, Dept Geog, Burlington, VT 05405 USA. [Penn, Colin A.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT USA. [Campbell, John L.] US Forest Serv, USDA, No Res Stn Durham, Durham, NH USA. RP Wemple, BC (reprint author), Univ Vermont, Dept Geog, Burlington, VT 05405 USA. EM bwemple@uvm.edu OI Campbell, John/0000-0003-4956-1696 FU National Sciences Foundation [ANT-0338008]; Vermont NASA EPSCoR program FX This work was supported by grants from the National Sciences Foundation through a Research Experience for Undergraduates supplement (ANT-0338008) to T. Neumann and B. Wemple and from the Vermont NASA EPSCoR program through a grant to J. Frolik, C. Skalka and B. Wemple. University of Vermont students M. Casari, S. Donovan, E. Furtak-Cole, T. Lanagan, E. Matys, H. Peterson, C. Robinson, N. Rustigian and P. Tobin assisted with field surveys. A. del Peral assisted with canopy photo analysis. USFS personnel Ian Halm, Tammy Wooster, and Derek Eaton assisted with transportation to field sites. W. Keeton provided the use of the camera. A. Whitehurst provided LiDAR-derived tree heights for the forested plots. A. McIntosh, G. Hawley, M. Pelto and two anonymous reviewers provided valuable comments of earlier versions of this manuscript. This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Hubbard Brook is part of the LTER network, which is supported by the National Science Foundation. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. NR 48 TC 3 Z9 3 U1 3 U2 40 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD AUG 15 PY 2012 VL 26 IS 17 SI SI BP 2524 EP 2534 DI 10.1002/hyp.9450 PG 11 WC Water Resources SC Water Resources GA 983ME UT WOS:000307122600003 ER PT J AU Ashworth, DJ Yates, SR Luo, LF Xuan, RC AF Ashworth, Daniel J. Yates, Scott R. Luo, Lifang Xuan, Richeng TI Phase partitioning, retention kinetics, and leaching of fumigant methyl iodide in agricultural soils SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Iodomethane; Mel; Fumigation; Soil organic matter; Virtually impermeable film ID PEST-CONTROL; 1,3-DICHLOROPROPENE; DEGRADATION; MOISTURE; SORPTION; DBCP AB Although it is not currently being sold in the USA, the recent US registration of the fumigant methyl iodide has led to an increased interest in its environmental fate and transport. Although some work has now considered its volatile emissions from soil, there remains a lack of experimental data regarding its ability to be retained in soil and ultimately become transported with irrigation/rain waters. Using laboratory batch and soil column experiments, we aimed to better understand the phase partitioning of Mel, the ability of soils to retain Mel on the solid phase, and the potential for leaching of Mel and its primary degradation product, iodide, down a soil profile. Results indicated that Mel was retained by the solid phase of soil, being protected from volatilization and degradation, particularly in the presence of elevated organic matter. Retention was greater at lower moisture content, and maximum retention occurred after 56 days of incubation. At higher moisture content, the liquid phase also became important in retaining Mel within soil. Together with low observed K-D values (0.10 to 0.57 mL, g(-1)). these data suggest that Mel may be prone to leaching. Indeed, in a steady-state soil column study, initially retained Mel was transported with interstitial water. The Mel degradation product, iodide, was also readily transported in this manner. The data highlight a potentially significant process by which Mel fate and transport within the environment may be impacted. (C) 2012 Elsevier By. All rights reserved. C1 [Ashworth, Daniel J.; Luo, Lifang; Xuan, Richeng] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ashworth, Daniel J.; Yates, Scott R.; Luo, Lifang; Xuan, Richeng] ARS, USDA, US Salin Lab, Riverside, CA 92507 USA. RP Ashworth, DJ (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. EM Daniel.Ashworth@ars.usda.gov NR 23 TC 0 Z9 0 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2012 VL 432 BP 122 EP 127 DI 10.1016/j.scitotenv.2012.06.006 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA 998WI UT WOS:000308270700014 PM 22728299 ER PT J AU Djousse, L Matthan, NR Lichtenstein, AH Gaziano, JM AF Djousse, Luc Matthan, Nirupa R. Lichtenstein, Alice H. Gaziano, John M. TI Red Blood Cell Membrane Concentration of cis-Palmitoleic and cis-Vaccenic Acids and Risk of Coronary Heart Disease SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID DE-NOVO LIPOGENESIS; MIDDLE-AGED ADULTS; STEAROYL-COA DESATURASE; FATTY-ACIDS; CARDIOVASCULAR-DISEASE; ATHEROSCLEROSIS RISK; PHYSICIANS HEALTH; MEN; MORTALITY; ONSET AB Although previous studies have suggested associations between plasma palmitoleic acid and coronary heart disease (CHD) risk factors, including blood pressure, inflammation, and insulin resistance, little is known about the relation of pahnitoleic acid and CHD. This ancillary study of the Physicians' Health Study was designed to examine whether red blood cell (RBC) membrane cis-pahnitoleic acid and cis-vaccenic acid-2 fatty acids that can be synthesized endogenously-are associated with CHD risk. We used a risk set sampling method to prospectively select 1,000 incident CHD events and 1,000 matched controls. RBC membrane fatty acids were measured using gas chromatography. The CHD cases were ascertained using an annual follow-up questionnaire and validated by an End Point Committee through a review of the medical records. In a conditional logistic regression analysis adjusting for demographics, anthropometric, lifestyle factors, and co-morbidity, the odds ratios and 95% confidence intervals (CIs) for CHD were 1.0 (referent), 1.29 (95% CI 0.95 to 1.75), 1.08 (95% CI 0.78 to 1.51), 1.25 (95% CI 0.90 to 1.75), and 1.48 (95% CI 1.03 to 2.14) across consecutive quintiles of RBC membrane cis-palmitoleic acid (p for trend = 0.041). The odds ratio associated with each SD higher RBC membrane cis-palmitoleic acid level was 1.19 (95% CI 1.06 to 1.35) in a multivariate-adjusted model. Finally, RBC membrane cis-vaccenic acid was inversely associated with CHD risk (odds ratio 0.79, 95% CI 0.69 to 0.91, per SD increase). In conclusion, our data showed a positive association between RBC membrane cis-pahnitoleic acid and CHD risk in male physicians. Furthermore, RBC membrane cis-vaccenic acid was inversely related to CHD. Published by Elsevier Inc. (Am J Cardiol 2012;110:539-544) C1 [Djousse, Luc; Gaziano, John M.] Brigham & Womens Hosp, Dept Med, Div Aging, Boston, MA 02115 USA. [Djousse, Luc; Gaziano, John M.] Harvard Univ, Sch Med, Boston, MA USA. [Djousse, Luc] Boston Vet Affairs Healthcare Syst, Geriatr Res Educ & Clin Ctr, Boston, MA USA. [Djousse, Luc] Boston Vet Affairs Healthcare Syst, Massachusetts Vet Epidemiol & Res Informat Ctr, Boston, MA USA. [Matthan, Nirupa R.; Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gaziano, John M.] Brigham & Womens Hosp, Div Prevent Med, Boston, MA 02115 USA. RP Djousse, L (reprint author), Brigham & Womens Hosp, Dept Med, Div Aging, 75 Francis St, Boston, MA 02115 USA. EM ldjousse@rics.bwh.harvard.edu FU National Heart, Lung, and Blood Institute (Bethesda, Maryland) [R21HL088081, HL-26490, HL-34595]; National Cancer Institute (Bethesda, Maryland) [CA-34944, CA-40360, CA-097193] FX The present ancillary study was funded by grant R21HL088081 (to L. Djousse) from the National Heart, Lung, and Blood Institute (Bethesda, Maryland). The Physicians' Health Study is Supported by grants CA-34944, CA-40360, and CA-097193 from the National Cancer Institute (Bethesda, Maryland) and grants HL-26490 and HL-34595 from the National Heart, Lung, and Blood Institute (Bethesda, Maryland). NR 29 TC 20 Z9 20 U1 1 U2 12 PU EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC PI BRIDGEWATER PA 685 ROUTE 202-206 STE 3, BRIDGEWATER, NJ 08807 USA SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD AUG 15 PY 2012 VL 110 IS 4 BP 539 EP 544 DI 10.1016/j.amjcard.2012.04.027 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 993LK UT WOS:000307861000013 PM 22579341 ER PT J AU Vomhof-DeKrey, EE Picklo, MJ AF Vomhof-DeKrey, Emilie E. Picklo, Matthew J. TI NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE Adipogenesis; Insulin signaling; Antioxidant response element; Electrophile response element; Oxidative stress; Obesity; miRNA regulation ID TRANSCRIPTION FACTOR NRF2; INCREASED OXIDATIVE STRESS; INDUCED INSULIN-RESISTANCE; HIGH-FAT DIET; NAD(P)H-QUINONE OXIDOREDUCTASE-1; MITOCHONDRIAL BIOGENESIS; ADIPOSE-TISSUE; IN-VITRO; OBESITY; DIFFERENTIATION AB The nuclear factor E2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) pathway responds to oxidative stress via control of several antioxidant defense gene expressions. Recent efforts demonstrate that Nrf2 modulates development of adiposity and adipogenesis. One of the major Nrf2-regulated proteins, NAD(P)H:quinone oxidoreductase 1 (NQO1), is implicated in the development of adipose tissue and obesity. However, little is known about in situ disposition of Nrf2, Keap1, and NQO1 during adipogenesis in isolated adipocytes. Based on literature data, we hypothesized that adipocyte differentiation would increase expression of the Nrf2/Keap1 pathway and NQO1. Using murine 3T3-L1 preadipocytes, we mapped an increase in NQO1 protein at limited clonal expansion and postmitotic growth arrest (Days 1-3) stages and a decrease in terminally differentiated (Day 8) adipocytes that lasted for several days afterward. Conversely, NQO1, Nrf2, and Keap1 mRNA expressions were all increased in differentiated adipocytes (Days 11-14), indicating a discrepancy between steady-state mRNA levels and resulting protein. Treatment of differentiated 3T3-L1 adipocytes with glycogen synthase kinase-3 beta (GSK-3 beta) inhibitor, LiCl, led to 1.9-fold increase in NQO1 protein. Sulforaphane enhanced NQO1 protein (10.5-fold) and blunted triglyceride and FABP4 accumulation. The decrement in triglyceride content was partially reversed when NQO1 activity was pharmacologically inhibited. These data demonstrate a biphasic response of Nrf2 and NQO1 during adipocyte differentiation that is regulated by Keap1- and GSK-3 beta-dependent mechanisms, and that hypertrophy is negatively regulated by NQO1 activity. Published by Elsevier Inc. C1 [Vomhof-DeKrey, Emilie E.; Picklo, Matthew J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Picklo, Matthew J.] Univ N Dakota, Dept Pharmacol Physiol & Therapeut, Grand Forks, ND 58201 USA. [Picklo, Matthew J.] Univ N Dakota, Dept Chem, Grand Forks, ND 58202 USA. RP Picklo, MJ (reprint author), USDA ARS, Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM matthew.picklo@ars.usda.gov FU USDA [5450-51000-048-00D] FX The authors are grateful to Kim Michelson, Mary Briske-Anderson, and Brenda Skinner for their technical assistance with this work. We thank Dr. David Siegel and Dr. David Ross, University of Colorado, Denver, Colorado, for the MAC220. This work was funded by USDA 5450-51000-048-00D. NR 53 TC 8 Z9 8 U1 1 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD AUG 15 PY 2012 VL 53 IS 4 BP 690 EP 700 DI 10.1016/j.freeradbiomed.2012.05.047 PG 11 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 994GY UT WOS:000307920100006 PM 22683604 ER PT J AU Hampel, D York, ER Allen, LH AF Hampel, Daniela York, Emily R. Allen, Lindsay H. TI Ultra-performance liquid chromatography tandem mass-spectrometry (UPLC-MS/MS) for the rapid, simultaneous analysis of thiamin, riboflavin, flavin adenine dinucleotide, nicotinamide and pyridoxal in human milk SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE B-vitamins; Human milk; Infant requirements; Lactation; UPLC-MS/MS ID WATER-SOLUBLE VITAMINS; INFANTS; VITAMERS; FORMS; FOODS; ASSAY AB A novel, rapid and sensitive ultra-performance liquid-chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of several B-vitamins in human milk was developed. Resolution by retention time or multiple reaction monitoring (MRM) for thiamin, riboflavin. Flavin adenine dinucleotide (FAD), nicotinamide and pyridoxal (PL) has been optimized within 2 min using a gradient of 10 mM ammonium formate (aq) and acetonitrile. Thiamin-(4-methyl-C-13-thiazol-5-yl-C-13(3)) hydrochloride, riboflavin-dioxo-pyrimidine-C-13(4),N-15(2), and pyridoxal-methyl-d(3) hydrochloride were used as internal standards. A sample-like matrix was found to be mandatory for the external standard curve preparation. C-13(3)-caffeine was added for direct assessment of analyte recovery. Intra- and inter-assay variability for all analytes ranged from 0.4 to 7.9% and from 2.2 to 5.2%, respectively. Samples were subjected to protein precipitation and removal of non-polar constituents by diethyl ether prior to analysis. Quantification was done by ratio response to the stable isotope labeled internal standards. The standard addition method determined recovery rates for each vitamin (73.0-100.2%). The limit of quantitation for all vitamins was between 0.05 and 5 ppb depending on the vitamin. Alternative approaches for sample preparation such as protein removal by centrifugal filter units, acetonitrile or trichloroacetic acid revealed low recovery and a greater coefficient of variation. Matrix effect studies indicated a significant influence by matrix constituents, showing the importance of stable isotope labeled internal standards for analyte quantitation in complex matrices. Published by Elsevier B.V. C1 [Hampel, Daniela; York, Emily R.; Allen, Lindsay H.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [York, Emily R.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Hampel, D (reprint author), USDA ARS, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM daniela.hampel@ars.usda.gov RI Hampel, Daniela/K-2047-2014 OI Hampel, Daniela/0000-0003-0288-7680 FU Bill and Melinda Gates Foundation [OPP53107]; USDA-ARS [007]; Gillings School of Public Health, University of North Carolina FX This work was funded by the Bill and Melinda Gates Foundation (OPP53107) and by intramural USDA-ARS Project #007. The authors gratefully thank Linda S. Adair and Margaret E. Bentley (Gillings School of Public Health, University of North Carolina) for providing funds for method development and breast milk samples, Reina Engle-Stone (Department of Nutrition, UC Davis), Yang Zhenyu (National Institute and Food Safety, Chinese Center for Disease Control and Prevention) and Christopher Duggan (Harvard School of Public Health) for making human milk samples available and Janet M. Peerson (Department of Nutrition, UC Davis) for the statistical analyses. USDA is an equal opportunity employer and provider. NR 34 TC 20 Z9 21 U1 1 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 J9 J CHROMATOGR B JI J. Chromatogr. B PD AUG 15 PY 2012 VL 903 BP 7 EP 13 DI 10.1016/j.jchromb.2012.06.024 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 995XH UT WOS:000308048100002 PM 22819611 ER PT J AU Du, WX Avena-Bustillos, RJ Woods, R Breksa, AP McHugh, TH Friedman, M Levin, CE Mandrell, R AF Du, Wen-Xian Avena-Bustillos, Roberto J. Woods, Rachelle Breksa, Andrew P. McHugh, Tara H. Friedman, Mendel Levin, Carol E. Mandrell, Robert TI Sensory Evaluation of Baked Chicken Wrapped with Antimicrobial Apple and Tomato Edible Films Formulated with Cinnamaldehyde and Carvacrol SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Antibacterial films and coatings; essential oils; cooked chicken; hedonic evaluation; sensory preferences ID PLANT ESSENTIAL OILS; ESCHERICHIA-COLI O157-H7; FRESH-CUT APPLES; ANTIBACTERIAL ACTIVITY; SALMONELLA-ENTERICA; PHYSICAL-PROPERTIES; STORAGE STABILITY; SHELF-LIFE; QUALITY; CHOLESTEROL AB The addition of plant essential oils to edible films and coatings has been shown to protect against bacterial pathogens and spoilage while also enhancing sensory properties of foods. This study evaluated the effect of adding 0.5 and 0.75% carvacrol (active ingredient of oregano oil) to apple- and tomato-based film-forming solutions and 0.5 and 0.75% cinnamaldehyde (active ingredient of cinnamon oil) to apple-based film-forming solutions on sensory properties of cooked chicken wrapped with these films. Paired preference tests indicated no difference between baked chicken wrapped with tomato and apple films containing 0.5% carvacrol and cinnamaldehyde compared to chicken wrapped with tomato or apple films without the plant antimicrobials. The taste panel indicated a higher preference for carvacrol-containing tomato-coated chicken over the corresponding apple coating. There was also a higher preference for cinnamaldehyde-containing apple films over corresponding carvacrol-containing wrapping. Films containing antibacterial active compounds derived from essential oils can be used to protect raw chicken pieces against bacterial contamination without adversely affecting preferences of wrapped chicken pieces after baking. C1 [Du, Wen-Xian; Avena-Bustillos, Roberto J.; Woods, Rachelle; Breksa, Andrew P.; McHugh, Tara H.; Friedman, Mendel; Levin, Carol E.; Mandrell, Robert] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Avena-Bustillos, RJ (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM roberto.avena@ars.usda.gov OI Levin, Carol/0000-0001-6522-6156; Friedman, Mendel/0000-0003-2582-7517 FU USDA, CSREES NRI [2006-35201-17409] FX This study was supported by the USDA, CSREES NRI Grant 2006-35201-17409. NR 33 TC 15 Z9 15 U1 3 U2 66 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 15 PY 2012 VL 60 IS 32 BP 7799 EP 7804 DI 10.1021/jf301281a PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 988JS UT WOS:000307487100005 PM 22831761 ER PT J AU Dia, VP Gomez, T Vernaza, G Berhow, M Chang, YK de Mejia, EG AF Dia, Vermont P. Gomez, Tegan Vernaza, Gabriela Berhow, Mark Chang, Yoon Kil de Mejia, Elvira Gonzalez TI Bowman-Birk and Kunitz Protease Inhibitors among Antinutrients and Bioactives Modified by Germination and Hydrolysis in Brazilian Soybean Cultivar BRS 133 SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; germination; Alcalase hydrolysis; antinutritional factors; bioactive compounds ID GLYCINE-MAX GERMINATION; IN-VITRO; CHEMICAL-COMPOSITION; ANTINUTRITIONAL FACTORS; NUTRITIONAL FACTORS; GAMMA-IRRADIATION; DIGESTIBILITY; SEEDS; ANTIOXIDANT; AGGLUTININ AB Soybean contains constituents that have antinutritional and bioactive properties. Enzymatic hydrolysis and germination can enhance the biological activity of these compounds in soybean. The objective of this study was to investigate the effect of germination, Alcalase (protease) hydrolysis, and their combination on the concentrations of antinutritional and bioactive compounds in Brazilian soybean cultivar BRS 133. A combination of germination and Alcalase hydrolysis resulted in the degradation of Bowman-Birk inhibitor (BBI), Kunitz trypsin inhibitor (KTI), and lunasin by 96.9, 97.8, and 38.4%. Lectin was not affected by any of the processing treatments when compared to nongerminated and nonhydrolyzed soy protein extract. Total isoflavones (ISF) and total saponins (SAP) increased by 16.2 and 28.7%, respectively, after 18 h of germination, while Alcalase hydrolysis led to the reduction of these compounds. A significant correlation was found between concentrations of BBI and KTI, BBI and lunasin, BBI and ISF, KTI and lunasin, KTI and ISF, KTI and SAP, lunasin and ISF, and ISF and SAP. Germination and Alcalase hydrolysis interacted in reducing BBI, ISF, and SAP. This study presents a process of preparing soy flour ingredients with lower concentrations of antinutritional factors and with biologically active constituents, important for the promotion of health associated with soybean consumption. In conclusion, 18 h of germination and 3 h of Alcalase hydrolysis is recommended for elimination of protease inhibitors, while bioactives are maintained by at least 50% of their original concentrations. C1 [Dia, Vermont P.; Gomez, Tegan; de Mejia, Elvira Gonzalez] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Vernaza, Gabriela; Chang, Yoon Kil] Univ Estadual Campinas, Dept Food Technol, Sch Food Engn, Campinas, SP, Brazil. [Berhow, Mark] Agr Res Serv, USDA, Peoria, IL 61604 USA. RP de Mejia, EG (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. EM edemejia@illinois.edu FU FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) FX We thank FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) for granting M.G.V. a postdoctoral fellowship. We thank Embrapa-Soybean-The National Center for Soybean Research, Brazil, and Embrapa Technology Transfer, Brazil, for the donation of soybean BRS 133. Also, we thank Drs. Ben O de Lumen and Lila O Vodkin for lunasin and lectin antibodies, respectively, and Dr. Anatoli Lygin for technical assistance in isoflavone analysis. NR 41 TC 11 Z9 12 U1 4 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 15 PY 2012 VL 60 IS 32 BP 7886 EP 7894 DI 10.1021/jf301926w PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 988JS UT WOS:000307487100017 PM 22800092 ER PT J AU Motta, D Abad, JD Langendoen, EJ Garcia, MH AF Motta, Davide Abad, Jorge D. Langendoen, Eddy J. Garcia, Marcelo H. TI A simplified 2D model for meander migration with physically-based bank evolution SO GEOMORPHOLOGY LA English DT Article DE Meander migration; Migration coefficient; Bank erosion; Planform shape; Computer model ID CHANNEL MIGRATION; RIVER MEANDERS; BEND THEORY; SEDIMENT TRANSPORT; STREAMBANK EROSION; INCISED STREAMS; SIMULATION; FORMULATION; FLOW AB The rate of migration, calculated by numerical models of river meandering, is commonly based on a method that relates the rate of migration to near-bank excess velocity multiplied by a dimensionless coefficient. Notwithstanding its simplicity, since the early 1980s this method has provided important insight into the long-term evolution of meander planforms through theoretical exercises. Its use in practice has not been as successful, because the complexity of the physical processes responsible for bank retreat, the heterogeneity in floodplain soils, and the presence of vegetation, make the calibration of the dimensionless coefficient rather challenging. This paper presents a new approach that calculates rates of meander migration using physically-based streambank erosion formulations. The University of Illinois RVR Meander model, which simulates meandering-river flow and bed morphodynamics, is integrated with algorithms for streambank erosion of the US Department of Agriculture channel evolution computer model CONCEPTS. The performance of the proposed approach is compared to that of the more simple classic method through the application to several test cases for idealized and natural planform geometry. The advantages and limitations of the approach are discussed, focusing on simulated planform pattern, the impact of soil spatial heterogeneity, the relative importance of the different processes controlling bank erosion (hydraulic erosion, cantilever, and planar failure), the requirements for obtaining stable migration patterns (centerline filtering and interpolation of bank physical properties), and the capability of predicting the planform evolution of natural rivers over engineering time scales (i.e., 50 to 100 years). The applications show that the improved physically-based method of bank retreat is required to capture the complex long-term migration patterns of natural channels, which cannot be merely predicted from hydrodynamics only. (C) 2011 Elsevier B.V. All rights reserved. C1 [Motta, Davide; Garcia, Marcelo H.] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. [Abad, Jorge D.] Univ Pittsburgh, Dept Civil & Environm Engn, Pittsburgh, PA 15261 USA. [Langendoen, Eddy J.] Agr Res Serv, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Motta, D (reprint author), Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. EM dmotta2@illinois.edu; jabad@pitt.edu; eddy.langendoen@ars.usda.gov; mhgarcia@illinois.edu OI Langendoen, Eddy/0000-0002-2215-4989 FU U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station; Bureau of Land Management FX This research was supported by an agreement from the U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station and using funds provided by the Bureau of Land Management through the sale of public lands as authorized by the Southern Nevada Public Land Management Act. This work was performed under Specific Cooperative Agreement No. 58-6408-8-265 between the Department of Civil and Environmental Engineering at the University of Illinois at Urbana-Champaign and the U.S. Department of Agriculture, Agricultural Research Service, National Sedimentation Laboratory. Inci Guneralp is gratefully acknowledged for providing the aerial pictures and the channel centerlines of the Mackinaw River. NR 57 TC 37 Z9 39 U1 1 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X J9 GEOMORPHOLOGY JI Geomorphology PD AUG 15 PY 2012 VL 163 SI SI BP 10 EP 25 DI 10.1016/j.geomorph.2011.06.036 PG 16 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 974PS UT WOS:000306449400002 ER PT J AU Ponder, F Fleming, RL Berch, S Busse, MD Elioff, JD Hazlett, PW Kabzems, RD Kranabetter, JM Morris, DM Page-Dumroese, D Palik, BJ Powers, RF Sanchez, FG Scott, DA Stagg, RH Stone, DM Young, DH Zhang, JW Ludovici, KH McKenney, DW Mossa, DS Sanborn, PT Voldseth, RA AF Ponder, Felix, Jr. Fleming, Robert L. Berch, Shannon Busse, Matt D. Elioff, John D. Hazlett, Paul W. Kabzems, Richard D. Kranabetter, J. Marty Morris, David M. Page-Dumroese, Deborah Palik, Brian J. Powers, Robert F. Sanchez, Felipe G. Scott, D. Andrew Stagg, Richard H. Stone, Douglas M. Young, David H. Zhang, Jianwei Ludovici, Kim H. McKenney, Daniel W. Mossa, Debbie S. Sanborn, Paul T. Voldseth, Richard A. TI Effects of organic matter removal, soil compaction and vegetation control on 10th year biomass and foliar nutrition: LTSP continent-wide comparisons SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Stand productivity; Long-Term Soil Productivity; Stem-only harvest; Full tree harvest; Forest floor removal; Climatic regions ID FOREST FLOOR REMOVAL; LOBLOLLY-PINE PLANTATIONS; SITE PREPARATION; LODGEPOLE PINE; NITROGEN DYNAMICS; UNITED-STATES; DOUGLAS-FIR; BRITISH-COLUMBIA; SEEDLING GROWTH; TREMBLING ASPEN AB We examined 10th year above-ground planted tree and total stand biomass, and planted tree foliar N and P concentrations across gradients in soil disturbance at 45 North American Long-Term Soil Productivity (LTSP) installations. While ranging across several climate regions, these installations all share a common experimental design with similar measurement protocols. Across all installations planted tree biomass with stem-only harvest (OM0), no compaction (C-0) and chemical vegetation control (VC), ranged from 2 to 90 Mg ha(-1). When compared with the OM0, full-tree harvest (OM1) had little consistent effect on any response variable. Full-tree harvest plus forest floor removal (OM2) also demonstrated few consistent effects on planted tree biomass, although Boreal - Great Lakes conifers showed some positive effects, reflecting high survival, but also negative effects on foliar nutrition. Compaction (C-2), regardless of OM treatment, increased planted tree stand biomass consistently in Warm Humid climates, and compaction with intact forest floors (OM0C2) did so across all regions. However, most installations had medium - or coarse-textured soils and compaction did not achieve theoretical growth-limiting bulk densities. Combining OM2 with C-2 resulted in lesser gains in planted tree biomass. Planted tree biomass gains with the OM0C2 were attributed largely to changes in physical soil characteristics, not to vegetation control or nutrient availability. Total stand biomass (Mg ha(-1)) was either unaffected or, with aspen, reduced by compaction. Vegetation control (VC) consistently enhanced planted tree biomass, regardless of climate, and also enhanced foliar nutrient concentrations on Warm Humid and Mediterranean sites. VC also increased total stand biomass on sites without abundant woody competitors, but decreased it on shrub-dominated Mediterranean sites. For many of the site types and species investigated, harvest-related organic matter removal and soil compaction (excepting aspen vegetative reproduction) have not resulted in large losses in stand biomass 10 year after harvest. Most stands, however, have not yet reached canopy closure, and treatment effects may continue to evolve. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved. C1 [Fleming, Robert L.; Hazlett, Paul W.; McKenney, Daniel W.; Mossa, Debbie S.] Great Lakes Forestry Ctr, Canadian Forest Serv, Sault Ste Marie, ON P6A 2E5, Canada. [Ponder, Felix, Jr.] Univ Missouri, USDA, US Forest Serv, No Res Stn, Columbia, MO 65211 USA. [Berch, Shannon; Kranabetter, J. Marty] British Columbia Minist Forests & Range, Res Branch Lab, Victoria, BC V8Z 5J3, Canada. [Busse, Matt D.; Powers, Robert F.; Zhang, Jianwei] US Forest Serv, USDA, Pacific SW Res Stn, Redding, CA 96001 USA. [Elioff, John D.; Palik, Brian J.; Stone, Douglas M.; Voldseth, Richard A.] US Forest Serv, USDA, No Res Stn, E Grand Rapids, MN 55744 USA. [Kabzems, Richard D.] British Columbia Minist Forests & Range, Ft St John, BC V1J 6M2, Canada. [Morris, David M.] Lakehead Univ, Ontario Minist Nat Resources, Ctr No Forest Ecosyst Res, Thunder Bay, ON P7B 5E1, Canada. [Page-Dumroese, Deborah] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Sanchez, Felipe G.; Ludovici, Kim H.] US Forest Serv, USDA, So Res Stn, Forestry Sci Lab, Res Triangle Pk, NC 27709 USA. [Scott, D. Andrew] US Forest Serv, USDA, So Res Stn, Normal, AL 35762 USA. [Stagg, Richard H.] US Forest Serv, USDA, So Res Stn, S Crossett, AR 71635 USA. [Sanborn, Paul T.] Univ No British Columbia, Prince George, BC V2N 4Z9, Canada. [Young, David H.] US Forest Serv, USDA, Redding, CA 96001 USA. [Voldseth, Richard A.] N Dakota State Univ, Dept Soil Sci, Fargo, ND 58105 USA. RP Fleming, RL (reprint author), Great Lakes Forestry Ctr, Canadian Forest Serv, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada. EM rob.fleming@nrcan-rncan.gc.ca NR 116 TC 36 Z9 36 U1 4 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2012 VL 278 BP 35 EP 54 DI 10.1016/j.foreco.2012.04.014 PG 20 WC Forestry SC Forestry GA 974RM UT WOS:000306454100005 ER PT J AU Wilson, TB Meyers, TP Kochendorfer, J Anderson, MC Heuer, M AF Wilson, T. B. Meyers, T. P. Kochendorfer, J. Anderson, M. C. Heuer, M. TI The effect of soil surface litter residue on energy and carbon fluxes in a deciduous forest SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Residues; LAI; Soil-vegetation-atmosphere system; Energy budget and carbon fluxes ID INTEGRATED BIOSPHERE SIMULATOR; TURBULENT EXCHANGE PROCESSES; NET PRIMARY PRODUCTION; CHOPPED CORN RESIDUE; LIGHT-USE EFFICIENCY; LEAF-AREA INDEX; PLANT CANOPIES; STOMATAL CONDUCTANCE; NITROGEN DYNAMICS; EDDY-COVARIANCE AB The Atmosphere-Land Exchange Surface Energy (ALEX) balance model is an analytical formulation of the energy and mass transport within the soil and the vegetation canopy used for simulating energy, evapotranspiration, and CO2 fluxes in a wide range of vegetation environments. The objective of this study was to evaluate the ability of ALEX to simulate the effect of soil-surface leaf litter residue on soil heat conduction (G), sensible heat (H), evapotranspiration (ET) (or latent heat (LE) when expressed as rate of energy loss) and CO2 fluxes in a deciduous forest. The model was evaluated in a deciduous forest in Oak Ridge, Tennessee where about 550 g m(-2) of dry weight of slow decomposing leaf litter is produced annually during the fall season. Incorporating an explicit formulation of water and energy exchanges within the residue layer in ALEX improved the performance of the model against eddy covariance and G measurements. The discrepancies between model simulations made with and without leaf litter residue were largest during the spring and fall, when soil contributions dominated the energy budget of the forest. During these periods, particularly during the spring, without the inclusion of the residue layer the model overpredicted LE, G, soil temperature and soil moisture, and underpredicted H. The model showed no differences in simulating above-canopy net radiation (RN), with a slight difference in the above-canopy CO2 flux. The largest model improvement for residue effects was in the simulation of G, with the slope of the regression line between predicted and measured values reduced from 2.28 for the model without residue effects to 1.07 when the residue effect was considered. (C) 2012 Elsevier B.V. All rights reserved. C1 [Wilson, T. B.; Meyers, T. P.; Kochendorfer, J.; Heuer, M.] NOAA, Atmospher Turbulence & Diffus Div, Oak Ridge, TN 37831 USA. [Anderson, M. C.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Wilson, TB (reprint author), NOAA, Atmospher Turbulence & Diffus Div, POB 2456, Oak Ridge, TN 37831 USA. EM tim.wilson@noaa.gov RI Kochendorfer, John/K-2680-2012; Anderson, Martha/C-1720-2015; Meyers, Tilden/C-6633-2016; Wilson, Timothy/C-9863-2016 OI Kochendorfer, John/0000-0001-8436-2460; Anderson, Martha/0000-0003-0748-5525; Wilson, Timothy/0000-0003-1785-5323 FU NOAA OAR/ARL FX This work was funded by the NOAA OAR/ARL Climate Research Program. NR 78 TC 5 Z9 7 U1 1 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD AUG 15 PY 2012 VL 161 BP 134 EP 147 DI 10.1016/j.agrformet.2012.03.013 PG 14 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 953CH UT WOS:000304844000013 ER PT J AU Li, GY Hse, CY Qin, TF AF Li, Gai-Yun Hse, Chung-Yun Qin, Te-Fu TI Preparation and characterization of novolak phenol formaldehyde resin from liquefied brown-rotted wood SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE brown-rotted wood; phenol liquefaction; novolak PF resin; molding ID LIQUEFACTION; ACID; CHEMISTRY AB The brown-rotted wood was liquefied in phenol with phosphoric acid as catalyst and the resulting liquefied products were condensed with formaldehyde to yield novolak liquefied wood-based phenol formaldehyde resin (LWPF). The results showed that brown-rotted wood could be more easily liquefied than sound wood in phenol. The residue content of liquefied wood decreased continually with the progress of brown-rot decay. Both water wash and neutralization and water wash slightly improved the thermofluidity and curing properties of LWPF with a small reduction of LWPF yield. An increase in phenol to wood (P/W) ratio from 2 to 3 slightly improved the flow property of LWPF, but with an accompanied by 20% deduction in the product yield. Increased liquefaction time from 30 min to 60 min showed no significant influence on the resulting LWPF. The moldings fabricated from LWPF yielded higher charpy impact, flexural and flexural modulus strength but yielded lower temperature deflection under load than that of the commercial novolak resin. (C) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 C1 [Li, Gai-Yun; Qin, Te-Fu] Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. [Hse, Chung-Yun] USDA Forest Serv, So Res Stn, Pineville, LA 71360 USA. RP Qin, TF (reprint author), Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. EM tefuqin@yahoo.cn FU National High Technology Research and Development Program of China (National 863 Project) [2010AA101703]; Central Public-interest Scientific Institution Basal Research Fund [CAFYBB2010003-4] FX Contract grant sponsor: National High Technology Research and Development Program of China (National 863 Project); contract grant number: 2010AA101703.; Contract grant sponsor: Central Public-interest Scientific Institution Basal Research Fund; contract grant number: CAFYBB2010003-4. NR 15 TC 7 Z9 7 U1 0 U2 17 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-8995 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD AUG 15 PY 2012 VL 125 IS 4 BP 3142 EP 3147 DI 10.1002/app.36476 PG 6 WC Polymer Science SC Polymer Science GA 936ND UT WOS:000303596200079 ER PT J AU Chao, WS Dogramaci, M Foley, ME Horvath, DP Anderson, JV AF Chao, Wun S. Dogramaci, Muenevver Foley, Michael E. Horvath, David P. Anderson, James V. TI Selection and Validation of Endogenous Reference Genes for qRT-PCR Analysis in Leafy Spurge (Euphorbia esula) SO PLOS ONE LA English DT Article ID TIME RT-PCR; HOUSEKEEPING GENES; QUANTITATIVE PCR; EXPRESSION; NORMALIZATION; QUANTIFICATION; ARABIDOPSIS; SEEDS; IDENTIFICATION; TRANSCRIPTOME AB Quantitative real-time polymerase chain reaction (qRT-PCR) is the most important tool in measuring levels of gene expression due to its accuracy, specificity, and sensitivity. However, the accuracy of qRT-PCR analysis strongly depends on transcript normalization using stably expressed reference genes. The aim of this study was to find internal reference genes for qRT-PCR analysis in various experimental conditions for seed, adventitious underground bud, and other organs of leafy spurge. Eleven candidate reference genes (BAM4, PU1, TRP-like, FRO1, ORE9, BAM1, SEU, ARF2, KAPP, ZTL, and MPK4) were selected from among 171 genes based on expression stabilities during seed germination and bud growth. The other ten candidate reference genes were selected from three different sources: (1) 3 stably expressed leafy spurge genes (60S, bZIP21, and MD-100) identified from the analyses of leafy spurge microarray data; (2) 3 orthologs of Arabidopsis "general purpose" traditional reference genes (GAPDH_1, GAPDH_2, and UBC); and (3) 4 orthologs of Arabidopsis stably expressed genes (UBC9, SAND, PTB, and F-box) identified from Affymetrix ATH1 whole-genome GeneChip studies. The expression stabilities of these 21 genes were ranked based on the C-T values of 72 samples using four different computation programs including geNorm, Normfinder, BestKeeper, and the comparative Delta C-T method. Our analyses revealed SAND, PTB, ORE9, and ARF2 to be the most appropriate reference genes for accurate normalization of gene expression data. Since SAND and PTB were obtained from 4 orthologs of Arabidopsis, while ORE9 and ARF2 were selected from 171 leafy spurge genes, it was more efficient to identify good reference genes from the orthologs of other plant species that were known to be stably expressed than that of randomly testing endogenous genes. Nevertheless, the two newly identified leafy spurge genes, ORE9 and ARF2, can serve as orthologous candidates in the search for reference genes from other plant species. C1 [Chao, Wun S.; Dogramaci, Muenevver; Foley, Michael E.; Horvath, David P.; Anderson, James V.] USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, Fargo, ND 58105 USA. RP Chao, WS (reprint author), USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, Fargo, ND 58105 USA. EM wun.chao@ars.usda.gov OI Anderson, James/0000-0002-1801-5767; Horvath, David/0000-0002-8458-7691 FU United States Department of Agriculture Agricultural Research Service CRIS Project [5442-21220-026-00] FX This work was supported by United States Department of Agriculture Agricultural Research Service CRIS Project # 5442-21220-026-00. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 36 TC 30 Z9 33 U1 1 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 14 PY 2012 VL 7 IS 8 AR e42839 DI 10.1371/journal.pone.0042839 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 988OQ UT WOS:000307500800024 PM 22916167 ER PT J AU Vrentas, CE Greenlee, JJ Tatum, TL Nicholson, EM AF Vrentas, Catherine E. Greenlee, Justin J. Tatum, Trudy L. Nicholson, Eric M. TI Relationships between PrPSc Stability and Incubation Time for United States Scrapie Isolates in a Natural Host System SO PLOS ONE LA English DT Article ID SHEEP PRION PROTEIN; SUFFOLK SHEEP; CONFORMATIONAL STABILITY; TRANSGENIC MICE; STRAIN; SUSCEPTIBILITY; TRANSMISSION; VARIANTS; GENOTYPE AB Transmissible spongiform encephalopathies (TSEs), including scrapie in sheep (Ovis aries), are fatal neurodegenerative diseases caused by the misfolding of the cellular prion protein (PrPC) into a a-rich conformer (PrPSc) that accumulates into higher-order structures in the brain and other tissues. Distinct strains of TSEs exist, characterized by different pathologic profiles upon passage into rodents and representing distinct conformations of PrPSc. One biochemical method of distinguishing strains is the stability of PrPSc as determined by unfolding in guanidine hydrochloride (GdnHCl), which is tightly and positively correlated with the incubation time of disease upon passage into mice. Here, we utilize a rapid, protease-free version of the stability assay to characterize naturally occurring scrapie samples, including a fast-acting scrapie inoculum for which incubation time is highly dependent on the amino acid at codon 136 of the prion protein. We utilize the stability methodology to identify the presence of two distinct isolates in the inoculum, and compare isolate properties to those of a host-stabilized reference scrapie isolate (NADC 13-7) in order to assess the stability/incubation time correlation in a natural host system. We demonstrate the utility of the stability methodology in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a range of natural host systems, including strains of bovine spongiform encephalopathy and chronic wasting disease. C1 [Vrentas, Catherine E.; Greenlee, Justin J.; Tatum, Trudy L.; Nicholson, Eric M.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Vrentas, CE (reprint author), USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. EM eric.nicholson@ars.usda.gov FU U.S. Department of Agriculture, Agriculture Research Service FX This research was funded in its entirety by congressionally appropriated funds to the U.S. Department of Agriculture, Agriculture Research Service. The funders of the work did not influence study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 4 Z9 4 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 14 PY 2012 VL 7 IS 8 AR e43060 DI 10.1371/journal.pone.0043060 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 988OQ UT WOS:000307500800040 PM 22916207 ER PT J AU Elumalai, S Tobimatsu, Y Grabber, JH Pan, XJ Ralph, J AF Elumalai, Sasikumar Tobimatsu, Yuki Grabber, John H. Pan, Xuejun Ralph, John TI Epigallocatechin gallate incorporation into lignin enhances the alkaline delignification and enzymatic saccharification of cell walls SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Lignification; Radical cross-coupling; Digestibility; Pretreatment; Saccharification ID PEROXIDASE-CATALYZED POLYMERIZATION; MONOLIGNOL BETA-GLYCOSIDES; IN-VITRO LIGNIFICATION; BIOFUEL PRODUCTION; DEHYDROGENATIVE POLYMERIZATIONS; O-METHYLTRANSFERASE; ETHANOL-PRODUCTION; HERBACEOUS PLANTS; DEFICIENT PLANTS; TRANSGENIC TREES AB Background: Lignin is an integral component of the plant cell wall matrix but impedes the conversion of biomass into biofuels. The plasticity of lignin biosynthesis should permit the inclusion of new compatible phenolic monomers such as flavonoids into cell wall lignins that are consequently less recalcitrant to biomass processing. In the present study, epigallocatechin gallate (EGCG) was evaluated as a potential lignin bioengineering target for rendering biomass more amenable to processing for biofuel production. Results: In vitro peroxidase-catalyzed polymerization experiments revealed that both gallate and pyrogallyl (B-ring) moieties in EGCG underwent radical cross-coupling with monolignols mainly by beta-O-4-type cross-coupling, producing benzodioxane units following rearomatization reactions. Biomimetic lignification of maize cell walls with a 3:1 molar ratio of monolignols and EGCG permitted extensive alkaline delignification of cell walls (72 to 92%) that far exceeded that for lignified controls (44 to 62%). Alkali-insoluble residues from EGCG-lignified walls yielded up to 34% more glucose and total sugars following enzymatic saccharification than lignified controls. Conclusions: It was found that EGCG readily copolymerized with monolignols to become integrally cross-coupled into cell wall lignins, where it greatly enhanced alkaline delignification and subsequent enzymatic saccharification. Improved delignification may be attributed to internal trapping of quinone-methide intermediates to prevent benzyl ether cross-linking of lignin to structural polysaccharides during lignification, and to the cleavage of ester intra-unit linkages within EGCG during pretreatment. Overall, our results suggest that apoplastic deposition of EGCG for incorporation into lignin would be a promising plant C1 [Elumalai, Sasikumar; Pan, Xuejun; Ralph, John] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Tobimatsu, Yuki; Ralph, John] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA. [Grabber, John H.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Ralph, John] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Ralph, John] Univ Wisconsin, Wisconsin Bioenergy Initiat, Madison, WI 53706 USA. RP Pan, XJ (reprint author), Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. EM xpan@wisc.edu; jralph@wisc.edu FU Stanford University's Global Climate and Energy Project (GCEP); US DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494]; Japan Society for the Promotion of Science (JSPS) FX The authors thank Christy Davidson and Hoon Kim for providing assistance with preparing and characterizing lignified cell walls. This work was supported primarily by a grant to XP and JR from Stanford University's Global Climate and Energy Project (GCEP). The authors also acknowledge partial funding from the US DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). YT gratefully acknowledges Postdoctoral Fellowship support from the Japan Society for the Promotion of Science (JSPS). NR 63 TC 14 Z9 14 U1 1 U2 40 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD AUG 13 PY 2012 VL 5 AR 59 DI 10.1186/1754-6834-5-59 PG 13 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA 023SA UT WOS:000310054300001 PM 22889353 ER PT J AU Dubey, JP Hill, DE Rozeboom, DW Rajendran, C Choudhary, S Ferreira, LR Kwok, OCH Su, C AF Dubey, J. P. Hill, D. E. Rozeboom, D. W. Rajendran, C. Choudhary, S. Ferreira, L. R. Kwok, O. C. H. Su, C. TI High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USA SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Organic pigs; Antibodies; Isolation; Genotyping ID UNITED-STATES; PRODUCTION SYSTEMS; RISK-FACTORS; INFECTION; SEROPREVALENCE; PORK; IDENTIFICATION; TRICHINELLA; MARYLAND; ILLNESS AB The ingestion of undercooked pork infected with Toxoplasma gondii is considered an important source of transmission of this parasite. While T. gondii infection in confinement raised market pigs (market pigs are typically used for fresh, unprocessed pork products) in the USA has decreased significantly over the last 20 years, infection levels in pigs with access to the outdoors can be quite high. An upsurge in consumer demand for 'organically raised', 'humanely raised' and 'free range' pork products has resulted in increasing numbers of hogs being raised in non-confinement systems. To determine T. gondii infection rate in these organic pigs, prevalence of T. gondii in organically raised pigs in two establishments (Farm 1, Farm 2) in Michigan was investigated. Serum and tissue samples from 33 pigs on the farm were available for T. gondii evaluation at slaughter. Serological testing was performed using both ELISA and the modified agglutination test (MAT). Antibodies to T. gondii were detected by both ELISA and MAT in 30 of 33 animals with MAT titers of 1:25 in three, 1:50 in six, 1:100 in seven, 1:200 in 13, and 1:400 in one. Hearts of all 33 pigs were bioassayed for T. gondii in mice; T. gondii was isolated from 17 pigs including one from a seronegative (both ELISA and MAT) pig. Genetic typing of 16 of the 17 T. gondii isolates using the SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, 1358, PK1 and Apico loci revealed clonal Type II from Farm 1 and clonal Type III on Farm 2. These results revealed very high prevalence of T. gondii in organic pigs for the first time in USA, indicating potentially increased health risk of consuming organic swine products. Published by Elsevier B.V. C1 [Dubey, J. P.; Hill, D. E.; Rajendran, C.; Choudhary, S.; Ferreira, L. R.; Kwok, O. C. H.] Agr Res Serv, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Rozeboom, D. W.] Michigan State Univ, Dept Anim Sci, Coll Agr & Nat Resources, E Lansing, MI 48824 USA. [Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), Agr Res Serv, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Su, Chunlei/M-1892-2013 OI Su, Chunlei/0000-0001-8392-7108 NR 28 TC 32 Z9 34 U1 2 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 13 PY 2012 VL 188 IS 1-2 BP 14 EP 18 DI 10.1016/j.vetpar.2012.03.008 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 976WJ UT WOS:000306617800003 PM 22483557 ER PT J AU Sukhumavasi, W Bellosa, ML Lucio-Forster, A Liotta, JL Lee, ACY Pornmingmas, P Chungpivat, S Mohammed, HO Lorentzen, L Dubey, JP Bowman, DD AF Sukhumavasi, Woraporn Bellosa, Mary L. Lucio-Forster, Araceli Liotta, Janice L. Lee, Alice C. Y. Pornmingmas, Pitcha Chungpivat, Sudchit Mohammed, Hussni O. Lorentzen, Leif Dubey, J. P. Bowman, Dwight D. TI Serological survey of Toxoplasma gondii, Dirofilaria immitis, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) infections in pet cats in Bangkok and vicinities, Thailand SO VETERINARY PARASITOLOGY LA English DT Article DE Heartworm; Toxoplasma gondii; Southeast Asia; SNAP; Retrovirus; Cat; ELISA; Modified agglutination test; Epidemiology ID STRAY CATS; RETROVIRUS INFECTIONS; PENINSULAR MALAYSIA; ABCD GUIDELINES; DOMESTIC CATS; SEROPREVALENCE; PREVENTION; DOGS; EPIDEMIOLOGY; ANTIBODIES AB The seroprevalence of Toxoplasma gondii, Dirofilaria immitis (heartworm), feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections was examined using serum or plasma samples from 746 pet cats collected between May and July 2009 from clinics and hospitals located in and around Bangkok, Thailand. The samples were tested for heartworm, Fly, and FeLV using a commercial ELISA. Of the 746 samples, 4.6% (34/746) were positive for heartworm antigen, 24.5% (183/746) had circulating FeLV antigen, and 20.1% (150/746) had antibodies against Fly. In addition, the first 348 submitted samples were tested for T. gondii antibodies using a modified agglutination test (MAT, cut off 1:25); 10.1% (35/348) were seropositive. Of the 348 cats sampled for all four pathogens, 11, 10, and 1 were positive for T. gondii antibodies and FIV antibodies, FeLV antigen, or D. immitis antigen. respectively. Of the 35 T. gondii-seropositive cats, 42.9% (15/35) were co-infected with at least one of the other three pathogens. The presence of antibodies to Fly was significantly associated with both age and gender, while FeLV antigen presence was only associated with age. In the case of FIV, males were twice as likely to be infected as females, and cats over 10 years of age were 13.5 times more likely to be infected than cats less than 1 year of age. FeLV antigen was more common in younger cats, with cats over 10 years of age being 10 times less likely to be FeLV positive than cats under 1 year of age. This is the first survey for these four pathogens affecting feline health in Thailand. (C) 2012 Elsevier B.V. All rights reserved. C1 [Sukhumavasi, Woraporn; Chungpivat, Sudchit] Chulalongkorn Univ, Fac Vet Sci, Dept Pathol, Parasitol Unit, Bangkok 10330, Thailand. [Bellosa, Mary L.; Lucio-Forster, Araceli; Liotta, Janice L.; Lee, Alice C. Y.; Bowman, Dwight D.] Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USA. [Pornmingmas, Pitcha] Suvarnachad Anim Hosp, Bangkok 10240, Thailand. [Mohammed, Hussni O.] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. [Lorentzen, Leif] IDEXX Labs, Westbrook, ME 04092 USA. [Dubey, J. P.] USDA, Anim Nat Resources Inst, Anim Parasit Dis Lab, BARC E, Beltsville, MD 20705 USA. RP Sukhumavasi, W (reprint author), Chulalongkorn Univ, Fac Vet Sci, Dept Pathol, Parasitol Unit, Henri Dunant Rd, Bangkok 10330, Thailand. EM vetkwan@hotmail.com OI Lee, Alice/0000-0002-6506-684X FU Chulalongkorn University FX The authors would like to thank IDEXX for supplying the ELISA tests. This work was supported in part by the grants for development of new faculty staff, Ratchadaphiseksomphot endowment fund, Chulalongkorn University. NR 43 TC 4 Z9 6 U1 2 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 13 PY 2012 VL 188 IS 1-2 BP 25 EP 30 DI 10.1016/j.vetpar.2012.02.021 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 976WJ UT WOS:000306617800005 PM 22497870 ER PT J AU Cassida, KA Lester, EC Foster, JG Turner, KE AF Cassida, K. A. Lester, E. C. Foster, J. G. Turner, K. E. TI Recirculating elutriator for extracting gastrointestinal nematode larvae from pasture herbage samples SO VETERINARY PARASITOLOGY LA English DT Article DE Grazing management; Larvae; Nematode larvae recovery; Sheep-nematoda ID HAEMONCHUS-CONTORTUS; TRICHOSTRONGYLID LARVAE; SHEEP; PRODUCTIVITY; POPULATIONS; MIGRATION; RUMINANTS; RECOVERY; SURVIVAL; LAMBS AB Gastrointestinal nematode (GIN) parasites present an important limitation to ruminant production worldwide. Methods for quantifying infective larvae of GIN on pastures are generally tedious, time-consuming, and require bulky equipment set-ups. This limitation to expedient data collection is a bottleneck in development of pasture management practices that might reduce pasture infectivity. We modified a soil elutriator concept for extracting GIN larvae from fresh herbage samples. Elutriators were constructed from readily available parts and compared to the Baermann funnel sedimentation method for larvae extraction. More samples could be extracted per day in the elutriator than in a Baermann unit with extraction times of 8 min versus 24 h, respectively. Accuracy, measured as maximum recovery of larvae seeded onto herbage samples, did not differ between extraction methods (62.3 vs. 69.8% for elutriator and Baermann, respectively, P>0.05). Larvae recovery from herbage in elutriators showed a strong log(e) relationship with extraction time (r(2) > 0.98), which will allow development of accurate correction factors for specific herbages to predict total larvae densities at extraction times less than those needed for maximum recovery. An extraction time of 8 min per sample gave the best compromise of speed, accuracy, and precision as measured by regression confidence bands and root mean square error of analysis of variance. Precision of the elutriator extraction for pasture samples was comparable to published methods and was not affected by forage species or canopy strata. The elutriator method sensitive enough to detect differences in. larvae density as small as 8 larvae g(-1) DM among pasture treatments. Elutriators extracted nematode larvae from herbage samples with accuracy and precision similar to existing methods, but did it much faster. Elutriation shows promise as a rapid method for extracting infective GIN larvae from pasture herbage. Published by Elsevier B.V. C1 [Cassida, K. A.; Lester, E. C.; Foster, J. G.; Turner, K. E.] Agr Res Serv, USDA, Appalachian Farming Syst Res Ctr, Beaver, WV 25813 USA. RP Cassida, KA (reprint author), Agr Res Serv, USDA, Appalachian Farming Syst Res Ctr, 1224 Airport Rd, Beaver, WV 25813 USA. EM kacassida@gmail.com NR 19 TC 1 Z9 1 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 13 PY 2012 VL 188 IS 1-2 BP 60 EP 67 DI 10.1016/j.vetpar.2012.03.007 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 976WJ UT WOS:000306617800010 PM 22497869 ER PT J AU Arthur, FH Fontenot, EA AF Arthur, Frank H. Fontenot, Emily A. TI Residual activity of methoprene and novaluron as surface treatments to manage the flour beetles, Tribolium castaneum and Tribolium confusum SO JOURNAL OF INSECT SCIENCE LA English DT Article DE efficacy; insecticides; surfaces ID CHITIN SYNTHESIS INHIBITOR; STORED-PRODUCT INSECTS; RHYZOPERTHA-DOMINICA; EFFICACY; COLEOPTERA; TENEBRIONIDAE; LEPIDOPTERA; HYDROPRENE; INSECTICIDES; CONCRETE AB The juvenile hormone analog methoprene, and the chitin synthesis inhibitor novaluron, were evaluated by exposing late-stage larvae of Tribolium castaneum (Herbst) or Tribolium confusum (Jacqueline DuVal) (Coleoptera: Tenebrionidae) to it. The larvae were exposed to it in food material, on concrete, on plywood, and on floor tile. Larvae of T. castaneum were more susceptible than T. confusum larvae to both methoprene and novaluron on all surfaces. A further evaluation was done by exposing adult T. confusum to methoprene and novaluron through food placed on concrete treated with methoprene and novaluron, and then assessing resulting progeny production. The emergence of adults with normal morphology was reduced for both chemicals, with more malformed adults appearing in the methoprene treatment, and fewer adults of any form emerging in the novaluron treatment. The results show direct exposures to larvae, or determining progeny production from exposed adults, are valid methods for assessing the susceptibility of flour beetles to insecticides. C1 [Arthur, Frank H.; Fontenot, Emily A.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Arthur, FH (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS USA. EM frank.arthur@ars.usda.gov; eagnesfontenot@gmail.com FU Central Sciences International, Schaumberg Park, IL; BASF Corporation, Research Triangle Park, NC FX We thank Central Sciences International, Schaumberg Park, IL, and BASF Corporation, Research Triangle Park, NC, for providing partial support of the research, and for providing the insecticides used in the study (methoprene and novaluron, respectively). This paper reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information, and does not imply recommendation or endorsement by the U. S. Department of Agriculture. The USDA is an equal opportunity provider and employer. We also thank P. Fields for reviewing an earlier draft of this paper. NR 29 TC 9 Z9 9 U1 1 U2 7 PU UNIV ARIZONA PI TUCSON PA LIBRARY C327, TUCSON, AZ 85721 USA SN 1536-2442 J9 J INSECT SCI JI J Insect Sci. PD AUG 12 PY 2012 VL 12 AR 95 PG 11 WC Entomology SC Entomology GA 988GF UT WOS:000307476400002 PM 23421852 ER PT J AU Derstine, NT Troyer, EJ Suttles, CN Siderhurst, LA Jang, EB Siderhurst, MS AF Derstine, Nathan T. Troyer, Elisa J. Suttles, Caitlyn N. Siderhurst, Leigh A. Jang, Eric B. Siderhurst, Matthew S. TI Field trapping the little fire ant, Wasmannia auropunctata SO JOURNAL OF INSECT SCIENCE LA English DT Article DE ant detection and monitoring; double-sided tape; microhabitat preferences; one-way trap; pheromone; species-specific trapping; 2,5-dimethyl-3-(2-methylbutyl)pyrazine ID BACTROCERA-DORSALIS; ROGER HYMENOPTERA; PLASTIC MATRIX; FORMICIDAE; FOREST; ALKYLPYRAZINES; ERADICATION; MANAGEMENT; DIVERSITY; HAWAII AB Two detection methods for the little fire ant, Wasmannia auropunctata (Roger) (Hymenoptera: Formicidae), both employing the pheromone attractant 2,5-dimethyl-3-(2-methylbutyl) pyrazine (2-MeBu-diMePy), were compared with peanut butter based detection, in order to evaluate differences in species specificity and detection reliability. Trapping was conducted using a transect through a macadamia orchard on the island of Hawaii. The transect consisted of a series of three-tree plots, each plot contained a peanut butter coated stick (the most common detection method used for W. auropunctata in Hawaii), a one-way trap treated with 2-MeBu-diMePy, and a piece of double-sided tape treated with 2-MeBu-diMePy. While there were no differences in the number of W. auropunctata counted with each detection method, and no differences in detection reliability (detecting the known presence of W. auropunctata in a plot), the pheromone-incorporating methods showed greater species specificity, retaining W. auropunctata almost exclusively. These results demonstrate the potential of pheromone-detection methods to selectively capture target ant species even when other ants are present and abundant. Combined data from all three detection methods and a previous visual survey along the transect showed a marked difference in the frequency of cohabitation among ant species. Of the 10 ant species collected, W. auropunctata was found as the sole ant species on a given tree at a significantly higher frequency than all other ant species except Pheidole fervens. 94% percent of the trees with W. auropunctata had only W. auropunctata, supporting previous observations that this species tends to displace other ant species. In addition, W. auropunctata microhabitat preferences were investigated using one-way traps containing 2-MeBu-diMePy, which were placed in three arboreal and three non-arboreal locations. While the number of ants captured did not differ by trap placement, when grouped, captures were significantly higher in arboreal than non-arboreal microhabitats. C1 [Derstine, Nathan T.; Troyer, Elisa J.; Suttles, Caitlyn N.; Siderhurst, Leigh A.; Siderhurst, Matthew S.] Eastern Mennonite Univ, Dept Chem, Harrisonburg, VA 22802 USA. [Jang, Eric B.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Siderhurst, MS (reprint author), Eastern Mennonite Univ, Dept Chem, 1200 Pk Rd, Harrisonburg, VA 22802 USA. EM nater06@gmail.com; elisa.troyer@gmail.com; caitlyn.suttles@emu.edu; siderhurst@gmail.com; eric.jang@ars.usda.gov; ms826@emu.edu FU Hawaii Invasive Species Council Research and Technology Grant Program [58148] FX We would like to thank Janice Nagata for assistance in conducting field experiments and collecting ants and Dr. Neil Reimer (Hawaii Department of Agriculture) for ant species identifications. This project was funded in part by grants from the Hawaii Invasive Species Council Research and Technology Grant Program (Agreement # 58148). NR 34 TC 4 Z9 4 U1 3 U2 20 PU UNIV ARIZONA PI TUCSON PA LIBRARY C327, TUCSON, AZ 85721 USA SN 1536-2442 J9 J INSECT SCI JI J Insect Sci. PD AUG 11 PY 2012 VL 12 AR 93 PG 13 WC Entomology SC Entomology GA 988GD UT WOS:000307476200001 PM 23421782 ER PT J AU Hawkes, WC Alkan, Z AF Hawkes, Wayne Chris Alkan, Zeynep TI Delayed Cell Cycle Progression in Selenoprotein W-depleted Cells Is Regulated by a Mitogen-activated Protein Kinase Kinase 4-p38/c-Jun NH2-terminal Kinase-p53 Pathway SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID N-TERMINAL KINASE; MAP KINASES; SIGNAL INTEGRATION; INDUCED APOPTOSIS; EPITHELIAL-CELLS; P53; JNK; CANCER; PHOSPHORYLATION; STRESS AB Selenoprotein W (SEPW1) is a ubiquitous, highly conserved thioredoxin-like protein whose depletion causes a transient p53- and p21(Cip1)-dependent G(1)-phase cell cycle arrest in breast and prostate epithelial cells. SEPW1 depletion increases phosphorylation of Ser-33 in p53, which is associated with decreased p53 ubiquitination and stabilization of p53. We report here that delayed cell cycle progression, Ser-33 phosphorylation, and p53 nuclear accumulation from SEPW1 depletion require mitogen-activated protein kinase kinase 4 (MKK4). Silencing MKK4 rescued G(1) arrest, Ser-33 phosphorylation, and nuclear accumulation of p53 induced by SEPW1 depletion, but silencing MKK3, MKK6, or MKK7 did not. SEPW1 silencing did not change the phosphorylation state of MKK4 but increased total MKK4 protein. Silencing p38 gamma, p38 delta, or JNK2 partially rescued G(1) arrest from SEPW1 silencing, suggesting they signal downstream from MKK4. These results imply that SEPW1 silencing increases MKK4, which activates p38 gamma, p38 delta, and JNK2 to phosphorylate p53 on Ser-33 and cause a transient G(1) arrest. C1 [Hawkes, Wayne Chris; Alkan, Zeynep] Univ Calif Davis, USDA, ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Hawkes, WC (reprint author), Univ Calif Davis, USDA, ARS, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM wayne.hawkes@ars.usda.gov FU United States Department of Agriculture CRIS Project [5306-51530-018-00D] FX This work was supported by United States Department of Agriculture CRIS Project 5306-51530-018-00D. NR 67 TC 15 Z9 16 U1 1 U2 9 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 10 PY 2012 VL 287 IS 33 BP 27371 EP 27379 DI 10.1074/jbc.M112.346593 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 993FF UT WOS:000307840700009 PM 22730327 ER PT J AU Rasooly, R He, XH Friedman, M AF Rasooly, Reuven He, Xiaohua Friedman, Mendel TI Milk Inhibits the Biological Activity of Ricin SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID IN-GROUND BEEF; SHIGA TOXIN; BINDING; LACTOSE; GALACTOSE; TOXICITY; SUBUNIT; CELLS; CHAIN; ASSAY AB Ricin is a highly toxic protein produced by the castor plant Ricinus communis. The toxin is relatively easy to isolate and can be used as a biological weapon. There is great interest in identifying effective inhibitors for ricin. In this study, we demonstrated by three independent assays that a component of reconstituted powdered milk has a high binding affinity to ricin. We discovered that milk can competitively bind to and reduce the amount of toxin available to asialofetuin type II, which is used as a model to study the binding of ricin to galactose cell-surface receptors. Milk also removes ricin bound to the microtiter plate. In parallel experiments, we demonstrated by activity assay and by immuno-PCR that milk can bind competitively to 1 ng/ml ricin, reducing the amount of toxin uptake by the cells, and thus inhibit the biological activity of ricin. The inhibitory effect of milk on ricin activity in Vero cells was at the same level as by anti-ricin antibodies. We also found that (a) milk did not inhibit ricin at concentrations of 10 or 100 ng/ml; (b) autoclaving 10 and 100 ng/ml ricin in DMEM at 121 degrees C for 30 min completely abolished activity; and (c) milk did not affect the activity of another ribosome inactivating protein, Shiga toxin type 2 (Stx2), produced by pathogenic Escherichia coli O157:H7. Unlike ricin, which is internalized into the cells via a galactose-binding site, Stx2 is internalized through the cell surface receptor glycolipid globotriasylceramides Gb3 and Gb4. These observations suggest that ricin toxicity may possibly be reduced at room temperature by a widely consumed natural liquid food. C1 [Rasooly, Reuven; He, Xiaohua] ARS, Foodborne Contaminants Unit, USDA, Albany, CA 94710 USA. [Friedman, Mendel] ARS, USDA, Unit Produce Safety & Microbiol Res, Albany, CA 94710 USA. RP Rasooly, R (reprint author), ARS, Foodborne Contaminants Unit, USDA, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov NR 32 TC 10 Z9 10 U1 0 U2 17 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 10 PY 2012 VL 287 IS 33 BP 27924 EP 27929 DI 10.1074/jbc.M112.362988 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 993FF UT WOS:000307840700066 PM 22733821 ER PT J AU Ma, M Yan, Y Huang, L Chen, MS Zhao, HX AF Ma, Meng Yan, Yan Huang, Li Chen, Mingshun Zhao, Huixian TI Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes SO BMC PLANT BIOLOGY LA English DT Article DE Triticum aestivum; Spike; Grain; Barley stripe mosaic virus (BSMV); Virus-induced gene silencing (VIGS); Functional genomics ID STRIPE-MOSAIC-VIRUS; SEED STORAGE PROTEINS; GLUTENIN; BARLEY; RNA; TOMATO; DNA; BIOSYNTHESIS; ARABIDOPSIS; SUBUNITS AB Background: The Barley stripe mosaic virus (BSMV)-based vector has been developed and used for gene silencing in barley and wheat seedlings to assess gene functions in pathogen-or insect-resistance, but conditions for gene silencing in spikes and grains have not been evaluated. In this study, we explored the feasibility of using BSMV for gene silencing in wheat spikes or grains. Results: Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after13 days post inoculation (dpi), and persisted until 30dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV: PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction of endogenous PDS transcript abundances, suggesting that BSMV: PDS was able to induce PDS silencing in wheat spikes and grains. Inoculation onto wheat spikes from heading to flowering stage was optimal for efficient silencing of PDS in wheat spikes. Furthermore, we used the BSMV-based system to reduce the transcript level of 1Bx14, a gene encoding for High-molecular-weight glutenin subunit 1Bx14 (HMW-GS 1Bx14), by 97 % in the grains of the BSMV:1Bx14 infected spikes at 15dpi, compared with that in BSMV:00 infected spikes, and the reduction persisted until at least 25 dpi. The amount of the HMW-GS 1Bx14 was also detectably decreased. The percentage of glutenin macropolymeric proteins in total proteins was significantly reduced in the grains of 1Bx14-silenced plants as compared with that in the grains of BSMV:00 infected control plants, indicating that HMW-GS 1Bx14 is one of major components participating in the formation of glutenin macropolymers in wheat grains. Conclusion: This is one of the first reports of successful application of BSMV-based virus-induced-gene-silencing (VIGS) for gene knockdown in wheat spikes and grains and its application in functional analysis of the 1Bx14 gene. The established BSMV-VIGS system will be very useful in future research on functional analysis of genes contributing to grain quality and the metabolic networks in developing seeds of wheat. C1 [Ma, Meng; Yan, Yan; Zhao, Huixian] State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Ma, Meng; Yan, Yan; Zhao, Huixian] NW A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. [Huang, Li] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Chen, Mingshun] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Chen, Mingshun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Zhao, HX (reprint author), State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. EM hxzhao212@nwsuaf.edu.cn FU Natural Science Foundation of China [30871578] FX This work was financed by Natural Science Foundation of China (30871578). We would like to thank Jackie Campbell, a Ph.D student of Dr. Li Huang in the Department of Plant Sciences and Plant Pathology, Montana State University, for her suggestions and discussions in exploring the technical feasibility of using BSMV for gene silencing in wheat spikes or grains with the PDS marker gene. NR 51 TC 16 Z9 20 U1 2 U2 36 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 10 PY 2012 VL 12 AR 141 DI 10.1186/1471-2229-12-141 PG 13 WC Plant Sciences SC Plant Sciences GA 014TR UT WOS:000309399100002 PM 22882902 ER PT J AU Yuan, WP Liang, SL Liu, SG Weng, ES Luo, YQ Hollinger, D Zhang, HC AF Yuan, Wenping Liang, Shunlin Liu, Shuguang Weng, Ensheng Luo, Yiqi Hollinger, David Zhang, Haicheng TI Improving model parameter estimation using coupling relationships between vegetation production and ecosystem respiration SO ECOLOGICAL MODELLING LA English DT Article DE Bayesian inversion; Eddy covariance; Markov chain Monte Carlo; Gross primary production; Ecosystem respiration; Parameter estimation ID WATER-VAPOR EXCHANGE; EDDY COVARIANCE MEASUREMENTS; TERRESTRIAL CARBON FLUXES; SOIL RESPIRATION; GAS-EXCHANGE; INTERANNUAL VARIABILITY; PHOTOSYNTHETIC CAPACITY; REGIONAL APPLICATIONS; TEMPERATURE RESPONSE; SEASONAL-CHANGES AB Data assimilation techniques and inverse analysis have been applied to extract ecological knowledge from ecosystem observations. However, the number of parameters in ecosystem models that can be constrained is limited by conventional inverse analysis. This study aims to increase the number of parameters that can be constrained in parameter inversions by considering the internal relationships among ecosystem processes. Our previous study has reported thermal adaptation of net ecosystem exchange (NEE). Ecosystems tend to transfer from a carbon source to sink when the air temperature exceeds the mean annual temperature, and attain their maximum uptake when the temperature reaches the long-term growing season mean. Because NEE is the difference between gross primary production (GPP) and ecosystem respiration (ER), the adaptation of NEE indirectly indicates the coupling relationship between GPP and ER. Five assimilation experiments were conducted with (1) estimated GPP based on eddy flux measurements, (2) estimated GPP and coupling relationship between GPP and ER, (3) observed NEE measurements, (4) observed NEE measurements and internal relationship between GPP and ER and (5) observed NEE, estimated ER and GPP. The results show that the inversion method, using only estimated GPP based on eddy covariance towers, constrained 4 of 16 parameters in the terrestrial ecosystem carbon model, and the improved method using both GPP data and the internal relationship between GPP and ER allowed us to constrain 10 of 16 parameters. The improved method constrained the parameters for ER without additional ER observations, and accordingly improved the model performance substantially for simulating ER. Overall, our method enhances our ability to extract information from ecosystem observations and potentially reduces uncertainty for simulating carbon dynamics across the regional and global scales. (C) 2012 Elsevier B.V. All rights reserved. C1 [Yuan, Wenping; Liang, Shunlin; Zhang, Haicheng] Beijing Normal Univ, Coll Global Change & Earth Syst Sci, Beijing 100875, Peoples R China. [Yuan, Wenping; Liang, Shunlin] Chinese Acad Sci, Inst Remote Sensing Applicat, Beijing 100875, Peoples R China. [Liang, Shunlin] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Liu, Shuguang] US Geol Survey, Earth Resources Observat & Sci Ctr, Sioux Falls, SD 57198 USA. [Weng, Ensheng; Luo, Yiqi] Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA. [Hollinger, David] USDA, Forest Serv, No Res Stn, Durham, NH 03824 USA. [Yuan, Wenping; Liang, Shunlin] Beijing Normal Univ, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. RP Yuan, WP (reprint author), Beijing Normal Univ, Coll Global Change & Earth Syst Sci, Beijing 100875, Peoples R China. EM wenpingyuancn@yahoo.com RI Weng, Ensheng/E-4390-2012; Hollinger, David/G-7185-2012; liang, shunlin/C-2809-2015 OI Weng, Ensheng/0000-0002-1858-4847; FU National Key Basic Research and Development Plan of China [2012CB955501, 2010CB833504]; National High Technology Research and Development Program of China (863 Program) [2009AA122101]; Fundamental Research Funds for the Central Universities; Office of Science (BER), US Department of Energy [DE-AI02-07ER64355] FX This research was financially supported by National Key Basic Research and Development Plan of China (2012CB955501 and 2010CB833504), National High Technology Research and Development Program of China (863 Program) (2009AA122101) and the Fundamental Research Funds for the Central Universities. Howland research was supported by the Office of Science (BER), US Department of Energy under Interagency Agreement No. DE-AI02-07ER64355. NR 84 TC 20 Z9 20 U1 2 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD AUG 10 PY 2012 VL 240 BP 29 EP 40 DI 10.1016/j.ecolmodel.2012.04.027 PG 12 WC Ecology SC Environmental Sciences & Ecology GA 978UB UT WOS:000306770200003 ER PT J AU Vegge, A Lund, R Stoll, B Thymann, T Hartmann, B Jelsing, J Bering, SB Jiang, P Qvist, N Burrin, D Jeppesen, P Holst, J Sangild, P AF Vegge, A. Lund, R. Stoll, B. Thymann, T. Hartmann, B. Jelsing, J. Bering, S. B. Jiang, P. Qvist, N. Burrin, D. Jeppesen, P. Holst, J. Sangild, P. TI Glucagon-like peptide-2 improves function following intestinal resection in preterm piglets SO REGULATORY PEPTIDES LA English DT Meeting Abstract CT 19th International Symposium on Regulatory Peptides CY AUG 20-23, 2012 CL Copenhagen Univ, Fac Life Sci (LIFE), Copenhagen, DENMARK HO Copenhagen Univ, Fac Life Sci (LIFE) C1 [Vegge, A.; Lund, R.; Thymann, T.; Bering, S. B.; Sangild, P.] Univ Copenhagen, Fac Sci, Dept Human Nutr, Copenhagen, Denmark. [Stoll, B.; Burrin, D.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Hartmann, B.; Holst, J.] Univ Copenhagen, Fac Hlth & Med Sci, Dept Biomed Sci, DK-1168 Copenhagen, Denmark. [Jelsing, J.] Gubra ApS, Horsholm, Denmark. [Jiang, P.] Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China. [Qvist, N.] Odense Univ Hosp, Surg Dept A, Odense, Denmark. [Jeppesen, P.] Rigshosp, Dept Gastroenterol CA 2121, DK-2100 Copenhagen, Denmark. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-0115 J9 REGUL PEPTIDES JI Regul. Pept. PD AUG 10 PY 2012 VL 177 SU 1 BP S36 EP S36 DI 10.1016/j.regpep.2012.05.083 PG 1 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 978XF UT WOS:000306778400083 ER PT J AU Xiao, ZL Lester, GE Luo, YG Wang, Q AF Xiao, Zhenlei Lester, Gene E. Luo, Yaguang Wang, Qin TI Assessment of Vitamin and Carotenoid Concentrations of Emerging Food Products: Edible Microgreens SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Microgreens; phytonutrients; ascorbic acid; carotenoids; phylloquinone; tocopherols; HPLC ID SPINACIA-OLERACEA L.; PHYLLOQUINONE VITAMIN-K-1; LEAFY VEGETABLES; ASCORBIC-ACID; LEAVES; TOCOPHEROL; QUALITY; GROWTH AB Microgreens (seedlings of edible vegetables and herbs) have gained popularity as a new culinary trend over the past few years. Although small in size, microgreens can provide surprisingly intense flavors, vivid colors, and crisp textures and can be served as an edible garnish or a new salad ingredient. However, no scientific data are currently available on the nutritional content of microgreens. The present study was conducted to determine the concentrations of ascorbic acid, carotenoids, phylloquinone, and tocopherols in 25 commercially available microgreens. Results showed that different microgreens provided extremely varying amounts of vitamins and carotenoids. Total ascorbic acid contents ranged from 20.4 to 147.0 mg per 100 g fresh weight (FW), while beta-carotene, lutein/zeaxanthin, and violaxanthin concentrations ranged from 0.6 to 12.1, 1.3 to 10.1, and 0.9 to 7.7 mg/100 g FW, respectively. Phylloquinone level varied from 0.6 to 4.1 mu g/g FW; meanwhile, alpha-tocopherol and gamma-tocopherol ranged from 4.9 to 87.4 and 3.0 to 39.4 mg/100 g FW, respectively. Among the 25 microgreens assayed, red cabbage, cilantro, garnet amaranth, and green daikon radish had the highest concentrations of ascorbic acids, carotenoids, phylloquinone, and tocopherols, respectively. In comparison with nutritional concentrations in mature leaves (USDA National Nutrient Database), the microgreen cotyledon leaves possessed higher nutritional densities. The phytonutrient data may provide a scientific basis for evaluating nutritional values of microgreens and contribute to food composition database. These data also may be used as a reference for health agencies' recommendations and consumers' choices of fresh vegetables. C1 [Lester, Gene E.; Luo, Yaguang] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Xiao, Zhenlei; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Lester, GE (reprint author), ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM gene.lester@ars.usda.gov; wangqin@umd.edu RI Socaciu, Carmen/P-8358-2014 OI Socaciu, Carmen/0000-0002-7352-5057 FU USDA-ARS [1265-43440-004-00] FX This study was supported by USDA-ARS Project 1265-43440-004-00. NR 25 TC 33 Z9 33 U1 2 U2 76 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 8 PY 2012 VL 60 IS 31 BP 7644 EP 7651 DI 10.1021/jf300459b PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 984SB UT WOS:000307210700011 PM 22812633 ER PT J AU Labate, JA Robertson, LD AF Labate, Joanne A. Robertson, Larry D. TI Evidence of cryptic introgression in tomato (Solanum lycopersicum L.) based on wild tomato species alleles SO BMC PLANT BIOLOGY LA English DT Article DE Cryptic introgression; Linkage drag; Breeding; DNA sequence; Solanum species ID POPULATION-STRUCTURE; MOLECULAR EVOLUTION; CROP IMPROVEMENT; MOSAIC-VIRUS; GENE; MARKERS; RESISTANCE; DIVERSITY; DOMESTICATION; RELATIVES AB Background: Many highly beneficial traits (e. g. disease or abiotic stress resistance) have been transferred into crops through crosses with their wild relatives. The 13 recognized species of tomato (Solanum section Lycopersicon) are closely related to each other and wild species genes have been extensively used for improvement of the crop, Solanum lycopersicum L. In addition, the lack of geographical barriers has permitted natural hybridization between S. lycopersicum and its closest wild relative Solanum pimpinellifolium in Ecuador, Peru and northern Chile. In order to better understand patterns of S. lycopersicum diversity, we sequenced 47 markers ranging in length from 130 to 1200 bp (total of 24 kb) in genotypes of S. lycopersicum and wild tomato species S. pimpinellifolium, Solanum arcanum, Solanum peruvianum, Solanum pennellii and Solanum habrochaites. Between six and twelve genotypes were comparatively analyzed per marker. Several of the markers had previously been hypothesized as carrying wild species alleles within S. lycopersicum, i.e., cryptic introgressions. Results: Each marker was mapped with high confidence (e<1 x 10(-30)) to a single genomic location using BLASTN against tomato whole genome shotgun chromosomes (SL2.40) database. Neighbor-joining trees showed high mean bootstrap support (86.8 +/- 2.34%) for distinguishing red-fruited from green-fruited taxa for 38 of the markers. Hybridization and parsimony splits networks, genomic map positions of markers relative to documented introgressions, and historical origins of accessions were used to interpret evolutionary patterns at nine markers with putatively introgressed alleles. Conclusion: Of the 47 genetic markers surveyed in this study, four were involved in linkage drag on chromosome 9 during introgression breeding, while alleles at five markers apparently originated from natural hybridization with S. pimpinellifolium and were associated with primitive genotypes of S. lycopersicum. The positive identification of introgressed genes within crop species such as S. lycopersicum will help inform conservation and utilization of crop germplasm diversity, for example, facilitating the purging of undesirable linkage drag or the exploitation of novel, favorable alleles. C1 [Labate, Joanne A.; Robertson, Larry D.] ARS, USDA, Plant Genet Resources Unit, Geneva, NY 14456 USA. RP Labate, JA (reprint author), ARS, USDA, Plant Genet Resources Unit, 630 W N St, Geneva, NY 14456 USA. EM joanne.labate@ars.usda.gov FU CRIS [1910-21000-019-00D] FX We thank S. Sheffer and P. Kisly for excellent technical support and E. Cobb for stimulating discussions. This work was funded by CRIS project 1910-21000-019-00D. USDA is an equal opportunity provider and employer. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 70 TC 10 Z9 11 U1 2 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 7 PY 2012 VL 12 AR 133 DI 10.1186/1471-2229-12-133 PG 12 WC Plant Sciences SC Plant Sciences GA 014TN UT WOS:000309398700001 PM 22871151 ER PT J AU Hicks, PD Hawthorne, KM Berseth, CL Marunycz, JD Heubi, JE Abrams, SA AF Hicks, Penni D. Hawthorne, Keli M. Berseth, Carol L. Marunycz, John D. Heubi, James E. Abrams, Steven A. TI Total calcium absorption is similar from infant formulas with and without prebiotics and exceeds that in human milk-fed infants SO BMC PEDIATRICS LA English DT Article ID BIRTH-WEIGHT INFANTS; BIOAVAILABILITY; METABOLISM; GIRLS; ZINC AB Background: 1) To evaluate calcium absorption in infants fed a formula containing prebiotics (PF) and one without prebiotics (CF). 2) To compare calcium absorption from these formulas with a group of human milk-fed (HM) infants. Methods: A dual tracer stable isotope method was used to assess calcium absorption in infants exclusively fed CF (n = 30), PF (n = 25) or HM (n = 19). Analysis of variance was used to analyze calcium intake, fractional calcium absorption, and the amount of calcium absorbed. Results: Calcium intake (Mean +/- SEM) for PF was 534 +/- 17 mg/d and 557 +/- 16 mg/d for CF (p = 0.33). Fractional calcium absorption was 56.8 +/- 2.6 % for PF and 59.2 +/- 2.3 % for CF (p = 0.49). Total calcium absorbed for PF was 300 +/- 14 mg/d and 328 +/- 13 mg/d for CF (p = 0.16). For HM infants calcium intake was 246 +/- 20 mg/d, fractional calcium absorption was 76.0 +/- 2.9 % and total calcium absorbed was 187 +/- 16 mg/d (p < 0.001, compared to either PF or CF). Conclusions: Despite lower fractional calcium absorption of CF and PF compared to HM, higher calcium content in both led to higher total calcium absorption compared to HM infants. No significant effect of prebiotics was observed on calcium absorption or other markers of bone mineral metabolism. C1 [Hicks, Penni D.; Hawthorne, Keli M.; Abrams, Steven A.] Rice Univ, Houston, TX 77005 USA. [Hicks, Penni D.; Hawthorne, Keli M.; Abrams, Steven A.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Hicks, Penni D.; Berseth, Carol L.; Marunycz, John D.] Mead Johnson Nutr, Evansville, IN USA. [Heubi, James E.] Cincinnati Childrens Hosp, Med Ctr, Cincinnati, OH USA. RP Hicks, PD (reprint author), Rice Univ, Houston, TX 77005 USA. EM penni@rice.edu OI Abrams, Steven/0000-0003-4972-9233 FU Mead-Johnson Nutrition; US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center; Department of Pediatrics, Baylor College of Medicine; Texas Children's Hospital, Houston, Texas; USDA/ARS [58-6250-6-001]; Institutional Clinical and Translational Science Award, NIH/NCRR [5UL1RR026314] FX Funding for this study was provided by Mead-Johnson Nutrition. Drs. Hicks, Heubi and Abrams and Ms. Hawthorne have no competing interests. Mr. Marunycz and Dr. Berseth are employees of Mead-Johnson Nutrition.; This work is a publication of the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Texas Children's Hospital, Houston, Texas. This project has been funded in part with federal funds from the USDA/ARS under Cooperative Agreement number 58-6250-6-001. This source of funding applies to each author. The funding body was not involved in any way in the study design, writing or interpretation of data. Contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. This project was supported by an Institutional Clinical and Translational Science Award, NIH/NCRR Grant Number 5UL1RR026314. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. We would also like to thank the following for assistance in the study: Maria Hamzo-Saliba, Michelle Taub, Sevahn Allahverdian, Adrianne Morse, Leslie Cruz, and Gloria Orozco. NR 16 TC 11 Z9 11 U1 1 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2431 J9 BMC PEDIATR JI BMC Pediatr. PD AUG 7 PY 2012 VL 12 AR 118 DI 10.1186/1471-2431-12-118 PG 6 WC Pediatrics SC Pediatrics GA 005HF UT WOS:000308740800001 PM 22871243 ER PT J AU Heekin, AM Guerrero, FD Bendele, KG Saldivar, L Scoles, GA Gondro, C Nene, V Djikeng, A Brayton, KA AF Heekin, Andrew M. Guerrero, Felix D. Bendele, Kylie G. Saldivar, Leo Scoles, Glen A. Gondro, Cedric Nene, Vishvanath Djikeng, Appolinaire Brayton, Kelly A. TI Analysis of Babesia bovis infection-induced gene expression changes in larvae from the cattle tick, Rhipicephalus (Boophilus) microplus SO PARASITES & VECTORS LA English DT Article ID PROBE LEVEL DATA; DERMACENTOR-VARIABILIS; MICROARRAY ANALYSIS; IMMUNE-SYSTEM; INHIBITOR; SUMMARIES; PROTEASES; ONTOLOGY; DATABASE; UNIPROT AB Background: Cattle babesiosis is a tick-borne disease of cattle that has severe economic impact on cattle producers throughout the world's tropical and subtropical countries. The most severe form of the disease is caused by the apicomplexan, Babesia bovis, and transmitted to cattle through the bite of infected cattle ticks of the genus Rhipicephalus, with the most prevalent species being Rhipicephalus (Boophilus) microplus. We studied the reaction of the R. microplus larval transcriptome in response to infection by B. bovis. Methods: Total RNA was isolated for both uninfected and Babesia bovis-infected larval samples. Subtracted libraries were prepared by subtracting the B. bovis-infected material with the uninfected material, thus enriching for expressed genes in the B. bovis-infected sample. Expressed sequence tags from the subtracted library were generated, assembled, and sequenced. To complement the subtracted library method, differential transcript expression between samples was also measured using custom high-density microarrays. The microarray probes were fabricated using oligonucleotides derived from the Bmi Gene Index database (Version 2). Array results were verified for three target genes by real-time PCR. Results: Ticks were allowed to feed on a B. bovis-infected splenectomized calf and on an uninfected control calf. RNA was purified in duplicate from whole larvae and subtracted cDNA libraries were synthesized from Babesia-infected larval RNA, subtracting with the corresponding uninfected larval RNA. One thousand ESTs were sequenced from the larval library and the transcripts were annotated. We used a R. microplus microarray designed from a R. microplus gene index, BmiGI Version 2, to look for changes in gene expression that were associated with infection of R. microplus larvae. We found 24 transcripts were expressed at a statistically significant higher level in ticks feeding upon a B. bovis-infected calf contrasted to ticks feeding on an uninfected calf. Six transcripts were expressed at a statistically significant lower level in ticks feeding upon a B. bovis-infected calf contrasted to ticks feeding on an uninfected calf. C1 [Heekin, Andrew M.; Guerrero, Felix D.; Bendele, Kylie G.] ARS, USDA, Knipling Bushland US Livestock Insect Res Lab, Kerrville, TX 78028 USA. [Saldivar, Leo] Univ Texas El Paso, Dept Math, El Paso, TX 79968 USA. [Scoles, Glen A.] ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. [Gondro, Cedric] Univ New England, Inst Genet & Bioinformat, Armidale, NSW 2351, Australia. [Nene, Vishvanath; Djikeng, Appolinaire] ILRI, Nairobi, Kenya. [Nene, Vishvanath; Djikeng, Appolinaire] Biosci Eastern & Cent Africa BecA Hub, Nairobi, Kenya. [Brayton, Kelly A.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. RP Heekin, AM (reprint author), ARS, USDA, Knipling Bushland US Livestock Insect Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM Andrew.Heekin@ars.usda.gov RI gondro, cedric/B-4362-2010 OI gondro, cedric/0000-0003-0666-656X FU National Research Initiative of the USDA CSREES grant [2005-35604-15440] FX L.S. was supported by the National Research Initiative of the USDA CSREES grant #2005-35604-15440 (to K.A.B. and F.D.G.). This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation of endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 35 TC 12 Z9 12 U1 1 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD AUG 7 PY 2012 VL 5 AR 162 DI 10.1186/1756-3305-5-162 PG 12 WC Parasitology SC Parasitology GA 001XO UT WOS:000308494000001 PM 22871314 ER PT J AU Mitchell, CPJ Kolka, RK Fraver, S AF Mitchell, Carl P. J. Kolka, Randall K. Fraver, Shawn TI Singular and Combined Effects of Blowdown, Salvage Logging, and Wildfire on Forest Floor and Soil Mercury Pools SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID BOREAL FOREST; METHYL MERCURY; REDUCED SULFUR; ORGANIC-CARBON; CLIMATE-CHANGE; FIRE; BIOMASS; LAKES; USA; EMISSIONS AB A number of factors influence the amount of mercury (Hg) in forest floors and soils, including deposition, volatile emission, leaching, and disturbances such as fire. Currently the impact on soil Hg pools from other widespread forest disturbances such as blowdown and management practices like salvage logging are unknown. Moreover, ecological and biogeochemical responses to disturbances are generally investigated within a single-disturbance context, with little currently known about the impact of multiple disturbances occurring in rapid succession. In this study we capitalize on a combination of blowdown, salvage logging and fire events in the sub-boreal region of northern Minnesota to assess both the singular and combined effects of these disturbances on forest floor and soil total Hg concentrations and pools. Although none of the disturbance combinations affected Hg in mineral soil, we did observe significant effects on both Hg concentrations and pools in the forest floor. Blowdown increased the mean Hg pool in the forest floor by 0.76 mg Hg m(-2) (223%). Salvage logging following blowdown created conditions leading to a significantly more severe floor burn during wildfire, which significantly enhanced Hg emission. This sequence of combined events resulted in a mean loss of approximately 0.42 mg Hg m(-2) (68% of pool) from the forest floor, after conservatively accounting for potential losses via enhanced soil leaching and volatile emissions between the disturbance and sampling dates. Fire alone or blowdown followed by fire did not significantly affect the total Hg concentrations or pools in the forest floor. Overall, unexpected consequences for soil Hg accumulation and by extension, atmospheric Hg emission and risk to aquatic biota, may result when combined impacts are considered in addition to singular forest floor and soil disturbances. C1 [Mitchell, Carl P. J.] Univ Toronto Scarborough, Dept Phys & Environm Sci, Toronto, ON M1C 1A4, Canada. [Kolka, Randall K.; Fraver, Shawn] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Mitchell, CPJ (reprint author), Univ Toronto Scarborough, Dept Phys & Environm Sci, 1265 Mil Trail, Toronto, ON M1C 1A4, Canada. EM carl.mitchell@utoronto.ca RI Mitchell, Carl/A-7212-2008 OI Mitchell, Carl/0000-0001-8538-5138 FU Joint Fire Science Program [08-1-5-04]; Natural Sciences and Engineering Research Council of Canada; Northern Research Station, U.S. Forest Service FX We thank N. Aspelin, S. Erlandson, K. Haynes, S. Jones, D. Kastendick, J. Kragthorpe, L. Patty, Z. Patty, and J. Smith for assistance in the field and/or laboratory, and we thank B. Anderson, M. Beckwith, K. McTighe, T. Norman, and B. Schueller (all Superior National Forest) for providing information regarding harvesting and fire-related activities. Funding was provided by the Joint Fire Science Program (Project 08-1-5-04), the Natural Sciences and Engineering Research Council of Canada, and the Northern Research Station, U.S. Forest Service. NR 68 TC 5 Z9 6 U1 2 U2 40 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 7 PY 2012 VL 46 IS 15 BP 7963 EP 7970 DI 10.1021/es300133h PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 984OG UT WOS:000307199800009 PM 22747193 ER PT J AU Hoh, E Dodder, NG Lehotay, SJ Pangallo, KC Reddy, CM Maruya, KA AF Hoh, Eunha Dodder, Nathan G. Lehotay, Steven J. Pangallo, Kristin C. Reddy, Christopher M. Maruya, Keith A. TI Nontargeted Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry Method and Software for Inventorying Persistent and Bioaccumulative Contaminants in Marine Environments SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID HALOGENATED 1'-METHYL-1,2'-BIPYRROLES MBPS; POLYBROMINATED DIPHENYL ETHERS; DIRECT SAMPLE INTRODUCTION; ORGANIC POLLUTANTS; NATURAL-PRODUCTS; DECHLORANE PLUS; GREAT-LAKES; FOOD-WEB; IDENTIFICATION; EXTRACTS AB Analytical methods for contaminant monitoring are generally targeted; i.e., they measure defined lists compounds. Routine monitoring projects using targeted methods are not usually designed to screen for unrecognized or novel contaminants and therefore miss compounds within the region or population of study that cause, or have the potential to cause, adverse biological impacts. We describe a nontargeted analytical method utilizing direct sample introduction coupled to comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry. To test the capabilities of this instrumental method within the context of marine contaminant surveys, we characterized a broad array of nonpolar, persistent, and bioaccumulative contaminants in Atlantic common dolphin (Delphinus delphis) blubber, including compounds that are not typically monitored. Compound identifications were made by searching a standard reference database, by contemporaneously analyzing mass spectra from reference standards, and by de novo interpretation. We identified a total of 271 compounds belonging to 24 classes; all compounds but 1 were halogenated. Anthropogenic contaminants and halogenated natural products were concurrently detected. A total of 86 compounds were anthropogenic contaminants that are not routinely targeted in environmental surveys, and 54 compounds were halogenated natural products. A total of 112 spectra were identified de novo, demonstrating that exclusive reliance on commercially available reference standards and mass spectral libraries may miss a significant fraction of identifiable compounds. We also cataloged 27 halogenated mass spectra that were not able to be identified. Due to the volume and complexity of the identification data, we developed custom software to organize and provide shared access to the identified mass spectra and related information. The nontargeted analytical method and data reporting system, in combination with the analysis of a high-trophic-level sentinel species, demonstrates a framework for creating an inventory of persistent and bioaccumulative contaminants in marine environments, with the future goal of suggesting new compounds for further investigation by targeted monitoring and risk assessment. C1 [Hoh, Eunha] San Diego State Univ, Grad Sch Publ Hlth, San Diego, CA 92182 USA. [Dodder, Nathan G.; Maruya, Keith A.] So Calif Coastal Water Res Project, Costa Mesa, CA 92626 USA. [Lehotay, Steven J.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Pangallo, Kristin C.] Colgate Univ, Dept Chem, Hamilton, NY 13446 USA. [Reddy, Christopher M.] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA. RP Hoh, E (reprint author), San Diego State Univ, Grad Sch Publ Hlth, 5500 Campanile Dr, San Diego, CA 92182 USA. EM ehoh@mail.sdsu.edu; nathand@sccwrp.org RI Dodder, Nathan/C-7971-2015 OI Dodder, Nathan/0000-0001-5913-1767 NR 45 TC 32 Z9 34 U1 3 U2 83 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 7 PY 2012 VL 46 IS 15 BP 8001 EP 8008 DI 10.1021/es301139q PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 984OG UT WOS:000307199800014 PM 22712571 ER PT J AU Abit, SM Bolster, CH Cai, P Walker, SL AF Abit, Sergio M. Bolster, Carl H. Cai, Peng Walker, Sharon L. TI Influence of Feedstock and Pyrolysis Temperature of Biochar Amendments on Transport of Escherichia coli in Saturated and Unsaturated Soil SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID POROUS-MEDIA; COLLOID TRANSPORT; ACTIVATED CARBON; ADSORPTION; BACTERIA; MANURE; SAND; HYDROPHOBICITY; DEPOSITION; RETENTION AB The effects of biochar feedstock, pyrolysis temperature, and application rate (1 and 2%) on the transport of two Escherichia coli isolates through a fine sand soil under water-saturated and partially saturated conditions were investigated in column experiments. Biochars from two feedstocks (poultry litter and pine chips) and pyrolyzed at two temperatures (350 and 700 degrees C) were evaluated. Both biochars pyrolyzed at 700 degrees C resulted in significant reductions in E. coli transport, with greater reductions observed with the pine chip biochars. For the low temperature biochars, increased transport was observed for the poultry litter biochar whereas reduced transport was observed for the pine chip biochar. In general, the effect of biochar application on E. coli transport was more pronounced in the unsaturated soils and for the 2% application rates. Large differences were also observed between the two isolates indicating that bacterial surface properties play a role in how biochar affects E. coli transport. C1 [Abit, Sergio M.; Bolster, Carl H.] Agr Res Serv, USDA, Bowling Green, KY 42104 USA. [Cai, Peng] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92507 USA. [Walker, Sharon L.] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. RP Bolster, CH (reprint author), Agr Res Serv, USDA, 230 Bennett Lane, Bowling Green, KY 42104 USA. EM carl.bolster@ars.usda.gov RI Cai, Peng/A-2314-2010 NR 45 TC 23 Z9 24 U1 9 U2 126 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 7 PY 2012 VL 46 IS 15 BP 8097 EP 8105 DI 10.1021/es300797z PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 984OG UT WOS:000307199800026 PM 22738035 ER PT J AU Harden, JW Koven, CD Ping, CL Hugelius, G McGuire, AD Camill, P Jorgenson, T Kuhry, P Michaelson, GJ O'Donnell, JA Schuur, EAG Tarnocai, C Johnson, K Grosse, G AF Harden, Jennifer W. Koven, Charles D. Ping, Chien-Lu Hugelius, Gustaf McGuire, A. David Camill, Phillip Jorgenson, Torre Kuhry, Peter Michaelson, Gary J. O'Donnell, Jonathan A. Schuur, Edward A. G. Tarnocai, Charles Johnson, Kristopher Grosse, Guido TI Field information links permafrost carbon to physical vulnerabilities of thawing SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article ID SOIL ORGANIC-CARBON; DYNAMICS; TUNDRA; PRODUCTIVITY; STORAGE AB Deep soil profiles containing permafrost (Gelisols) were characterized for organic carbon (C) and total nitrogen (N) stocks to 3 m depths. Using the Community Climate System Model (CCSM4) we calculate cumulative distributions of active layer thickness (ALT) under current and future climates. The difference in cumulative ALT distributions over time was multiplied by C and N contents of soil horizons in Gelisol suborders to calculate newly thawed C and N. Thawing ranged from 147 PgC with 10 PgN by 2050 (representative concentration pathway RCP scenario 4.5) to 436 PgC with 29 PgN by 2100 (RCP 8.5). Organic horizons that thaw are vulnerable to combustion, and all horizon types are vulnerable to shifts in hydrology and decomposition. The rates and extent of such losses are unknown and can be further constrained by linking field and modelling approaches. These changes have the potential for strong additional loading to our atmosphere, water resources, and ecosystems. Citation: Harden, J. W., et al. (2012), Field information links permafrost carbon to physical vulnerabilities of thawing, Geophys. Res. Lett., 39, L15704, doi: 10.1029/2012GL051958. C1 [Harden, Jennifer W.] US Geol Survey, Menlo Pk, CA 94025 USA. [Koven, Charles D.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA. [Ping, Chien-Lu; Michaelson, Gary J.] Univ Alaska Fairbanks, Palmer Res Ctr, Fairbanks, AK USA. [Hugelius, Gustaf; Kuhry, Peter] Stockholm Univ, Dept Phys Geog & Quaternary Geol, S-10691 Stockholm, Sweden. [McGuire, A. David] Univ Alaska Fairbanks, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK USA. [Camill, Phillip] Bowdoin Coll, Environm Studies Program, Brunswick, ME 04011 USA. [Camill, Phillip] Bowdoin Coll, Dept Earth & Oceanog Sci, Brunswick, ME 04011 USA. [Jorgenson, Torre] Alaska Ecosci, Fairbanks, AK USA. [O'Donnell, Jonathan A.] US Geol Survey, Boulder, CO USA. [Schuur, Edward A. G.] Univ Florida, Dept Biol, Gainesville, FL USA. [Tarnocai, Charles] Agr & Agri Food Canada, Ottawa, ON, Canada. [Johnson, Kristopher] USDA, Forest Serv, Newtown Sq, PA USA. [Grosse, Guido] Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK 99775 USA. RP Harden, JW (reprint author), US Geol Survey, 345 Middlefield Rd, Menlo Pk, CA 94025 USA. EM jharden@usgs.gov RI Grosse, Guido/F-5018-2011; Hugelius, Gustaf/C-9759-2011; Koven, Charles/N-8888-2014 OI Grosse, Guido/0000-0001-5895-2141; Hugelius, Gustaf/0000-0002-8096-1594; Koven, Charles/0000-0002-3367-0065 FU Vulnerability of Permafrost Carbon Research Coordination Network; USGS; DOE/BER [DE-AC02-05CH11231]; Swedish Research Council FX This collaboration was supported by the Vulnerability of Permafrost Carbon Research Coordination Network and its numerous funding agencies. We also acknowledge USGS, DOE/BER DE-AC02-05CH11231 and Swedish Research Council. We gratefully acknowledge helpful reviews by Julie Jastrow and Kristen Manies. NR 41 TC 87 Z9 87 U1 12 U2 142 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD AUG 7 PY 2012 VL 39 AR L15704 DI 10.1029/2012GL051958 PG 6 WC Geosciences, Multidisciplinary SC Geology GA 988BO UT WOS:000307463400002 ER PT J AU Gibbons, JG Salichos, L Slot, JC Rinker, DC McGary, KL King, JG Klich, MA Tabb, DL McDonald, WH Rokas, A AF Gibbons, John G. Salichos, Leonidas Slot, Jason C. Rinker, David C. McGary, Kriston L. King, Jonas G. Klich, Maren A. Tabb, David L. McDonald, W. Hayes Rokas, Antonis TI The Evolutionary Imprint of Domestication on Genome Variation and Function of the Filamentous Fungus Aspergillus oryzae SO CURRENT BIOLOGY LA English DT Article ID BIOSYNTHESIS GENE-CLUSTER; SACCHAROMYCES-CEREVISIAE; SECONDARY METABOLISM; LACTOCOCCUS-LACTIS; VOLATILE COMPOUNDS; RNA-SEQ; TELL US; FLAVUS; AFLATOXIN; WILD AB The domestication of animals, plants, and microbes fundamentally transformed the lifestyle and demography of the human species [1]. Although the genetic and functional underpinnings of animal and plant domestication are well understood, little is known about microbe domestication [2-6]. Here, we systematically examined genome-wide sequence and functional variation between the domesticated fungus Aspergillus oryzae, whose saccharification abilities humans have harnessed for thousands of years to produce sake, soy sauce, and miso from starch-rich grains, and its wild relative A. flavus, a potentially toxigenic plant and animal pathogen [7]. We discovered dramatic changes in the sequence variation and abundance profiles of genes and wholesale primary and secondary metabolic pathways between domesticated and wild relative isolates during growth on rice. Our data suggest that, through selection by humans, an atoxigenic lineage of A. flavus gradually evolved into a "cell factory" for enzymes and metabolites involved in the saccharification process. These results suggest that whereas animal and plant domestication was largely driven by Neolithic "genetic tinkering" of developmental pathways, microbe domestication was driven by extensive remodeling of metabolism. C1 [Gibbons, John G.; Salichos, Leonidas; Slot, Jason C.; Rinker, David C.; McGary, Kriston L.; King, Jonas G.; Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Rinker, David C.; Rokas, Antonis] Vanderbilt Univ, Ctr Human Genet Res, Nashville, TN 37235 USA. [Klich, Maren A.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Tabb, David L.] Vanderbilt Univ, Med Ctr, Dept Biomed Informat, Nashville, TN 37232 USA. [McDonald, W. Hayes] Vanderbilt Univ, Med Ctr, Dept Biochem, Nashville, TN 37232 USA. RP Rokas, A (reprint author), Vanderbilt Univ, Dept Biol Sci, 221 Kirkland Hall, Nashville, TN 37235 USA. EM antonis.rokas@vanderbilt.edu RI Slot, Jason/E-4802-2011; Rokas, Antonis/A-9775-2008; McDonald, W. Hayes/B-4109-2016; OI Rokas, Antonis/0000-0002-7248-6551; McDonald, W. Hayes/0000-0002-3510-426X; Tabb, David/0000-0001-7223-578X FU Graduate Program in Biological Sciences at Vanderbilt University; National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH) [NIAID: F31AI091343-01]; Searle Scholars Program; National Science Foundation [DEB-0844968] FX We thank members of the Rokas lab, Chris Hittinger, Shannon Beltz, David Geiser, Kathy Friedman, David Friedman, Jim Patton, Julian Hillyer, Kamya Rajaram, Abby Olena, Scott Egan, Jonathan Flowers, David McCauley, Travis Clark, Chelsea Baker, Osamu Yamada, and the National Research Institute of Brewing of Japan. J.G.G. is funded by the Graduate Program in Biological Sciences at Vanderbilt University and the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH, NIAID: F31AI091343-01). Research in A.R.'s lab is supported by the Searle Scholars Program and the National Science Foundation (DEB-0844968). NR 59 TC 35 Z9 35 U1 6 U2 59 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD AUG 7 PY 2012 VL 22 IS 15 BP 1403 EP 1409 DI 10.1016/j.cub.2012.05.033 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 987KE UT WOS:000307415000020 PM 22795693 ER PT J AU Smith, DR AF Smith, David R. TI The South American genus Lagideus Konow (Hymenoptera: Pergidae: Syzygoniinae), a Supplement SO ZOOTAXA LA English DT Article DE sawflies; new species; Central America; South America AB Six new species of the Neotropical pergid genus Lagideus are described and illustrated: Lagideus boyaca, L. magdalena, L. schmidti, and L. flavus from Colombia and L. tapanti, and L. isidro from Costa Rica. Lagideus romius Smith is newly recorded from Colombia and the female lancet is illustrated. Females are described for the first time for Lagideus longicus Smith from Costa Rica and L. albitarsis Malaise from southeastern Brazil, Uruguay, and Argentina. C1 ARS, Systemat Entomol Lab, PSI, USDA,Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. RP Smith, DR (reprint author), ARS, Systemat Entomol Lab, PSI, USDA,Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com FU National Science Foundation [DEB9972024, DEB0205982] FX The specimens from Colombia were acquired through the Colombian Arthropod Survey, National Science Foundation Grants DEB9972024 and DEB0205982 to Michael J. Sharkey, University of Kentucky, Lexington. I appreciate the loan of specimens from P. Hanson (UCR), A. Taeger and S. M. Blank (SDEI), and J. Wiley (FSCA). Michele Touchet, Systematic Entomology Laboratory, USDA, Washington, DC, helped with the images. I appreciate the comments of the following reviewers: S. M. McKamey and T J. Henry, Systematic Entomology Laboratory, USDA, Washington, D. C. USDA is an equal opportunity provider and employer. NR 7 TC 0 Z9 0 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD AUG 7 PY 2012 IS 3413 BP 1 EP 18 PG 18 WC Zoology SC Zoology GA 985BH UT WOS:000307237800001 ER PT J AU Xue, L Scott, HM Cohnstaedt, LW Scoglio, C AF Xue, Ling Scott, H. Morgan Cohnstaedt, Lee W. Scoglio, Caterina TI A network-based meta-population approach to model Rift Valley fever epidemics SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Meta-population; Deterministic model; Rift Valley fever (RVF); Weighted networks; Reproduction number ID TRANSMISSION; OUTBREAK; VIRUS; KENYA; DISEASES; SENEGAL; IMPACT; RISK AB Rift Valley fever virus (RVFV) has been expanding its geographical distribution with important implications for both human and animal health. The emergence of Rift Valley fever (RVF) in the Middle East, and its continuing presence in many areas of Africa, has negatively impacted both medical and veterinary infrastructures and human morbidity, mortality, and economic endpoints. Furthermore, worldwide attention should be directed towards the broader infection dynamics of RVFV, because suitable host, vector and environmental conditions for additional epidemics likely exist on other continents; including Asia, Europe and the Americas. We propose a new compartmentalized model of RVF and the related ordinary differential equations to assess disease spread in both time and space; with the latter driven as a function of contact networks. Humans and livestock hosts and two species of vector mosquitoes are included in the model. The model is based on weighted contact networks, where nodes of the networks represent geographical regions and the weights represent the level of contact between regional pairings for each set of species. The inclusion of human, animal, and vector movements among regions is new to RVF modeling. The movement of the infected individuals is not only treated as a possibility, but also an actuality that can be incorporated into the model. We have tested, calibrated, and evaluated the model using data from the recent 2010 RVF outbreak in South Africa as a case study; mapping the epidemic spread within and among three South African provinces. An extensive set of simulation results shows the potential of the proposed approach for accurately modeling the RVF spreading process in additional regions of the world. The benefits of the proposed model are twofold: not only can the model differentiate the maximum number of infected individuals among different provinces, but also it can reproduce the different starting times of the outbreak in multiple locations. Finally, the exact value of the reproduction number is numerically computed and upper and lower bounds for the reproduction number are analytically derived in the case of homogeneous populations. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Xue, Ling; Scoglio, Caterina] Kansas State Univ, Dept Elect & Comp Engn, Manhattan, KS 66506 USA. [Scott, H. Morgan] Kansas State Univ, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Cohnstaedt, Lee W.] USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Scoglio, C (reprint author), Kansas State Univ, Dept Elect & Comp Engn, Manhattan, KS 66506 USA. EM lxue@ksu.edu; hmscott@vet.k-state.edu; Lee.Cohnstaedt@ars.usda.gov; caterina@k-state.edu FU DHS Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD); National Agricultural Biosecurity Center (NABC) at Kansas State University FX This work has been supported by the DHS Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) and by the National Agricultural Biosecurity Center (NABC) at Kansas State University. We would like to give special thanks to the anonymous editor and reviewer for their comments. We are grateful to Jason Coleman, Regina M. Beard, Livia Olsen, and Kelebogile Olifant for their help on bibliography research. We would like to give thanks to Duygu Balcan, Phillip Schumm, Faryad Darabi Sahneh, Anton Lyubinin, and Getahun Agga for the help in producing the paper, and Kristine Bennett for answering questions on entomology. NR 43 TC 29 Z9 29 U1 1 U2 31 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD AUG 7 PY 2012 VL 306 BP 129 EP 144 DI 10.1016/j.jtbi.2012.04.029 PG 16 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA 966DP UT WOS:000305818100013 PM 22564391 ER PT J AU Hou, YL Bickhart, DM Hvinden, ML Li, CJ Song, JZ Boichard, DA Fritz, S Eggen, A DeNise, S Wiggans, GR Sonstegard, TS Van Tassell, CP Liu, GE AF Hou, Yali Bickhart, Derek M. Hvinden, Miranda L. Li, Congjun Song, Jiuzhou Boichard, Didier A. Fritz, Sebastien Eggen, Andre DeNise, Sue Wiggans, George R. Sonstegard, Tad S. Van Tassell, Curtis P. Liu, George E. TI Fine mapping of copy number variations on two cattle genome assemblies using high density SNP array SO BMC GENOMICS LA English DT Article DE Cattle genome; Breed; Copy number variation (CNV); Single nucleotide polymorphism (SNP) ID HIDDEN-MARKOV MODEL; GENOTYPING PLATFORMS; FUNCTIONAL IMPACT; BOVINE GENOME; MOUSE GENOME; BOS-TAURUS; MAP; SEQUENCE; IDENTIFICATION; POLYMORPHISMS AB Background: Btau_4.0 and UMD3.1 are two distinct cattle reference genome assemblies. In our previous study using the low density BovineSNP50 array, we reported a copy number variation (CNV) analysis on Btau_4.0 with 521 animals of 21 cattle breeds, yielding 682 CNV regions with a total length of 139.8 megabases. Results: In this study using the high density BovineHD SNP array, we performed high resolution CNV analyses on both Btau_4.0 and UMD3.1 with 674 animals of 27 cattle breeds. We first compared CNV results derived from these two different SNP array platforms on Btau_4.0. With two thirds of the animals shared between studies, on Btau_4.0 we identified 3,346 candidate CNV regions representing 142.7 megabases (similar to 4.70%) of the genome. With a similar total length but 5 times more event counts, the average CNVR length of current Btau_4.0 dataset is significantly shorter than the previous one (42.7 kb vs. 205 kb). Although subsets of these two results overlapped, 64% (91.6 megabases) of current dataset was not present in the previous study. We also performed similar analyses on UMD3.1 using these BovineHD SNP array results. Approximately 50% more and 20% longer CNVs were called on UMD3.1 as compared to those on Btau_4.0. However, a comparable result of CNVRs (3,438 regions with a total length 146.9 megabases) was obtained. We suspect that these results are due to the UMD3.1 assembly's efforts of placing unplaced contigs and removing unmerged alleles. Selected CNVs were further experimentally validated, achieving a 73% PCR validation rate, which is considerably higher than the previous validation rate. About 20-45% of CNV regions overlapped with cattle RefSeq genes and Ensembl genes. Panther and IPA analyses indicated that these genes provide a wide spectrum of biological processes involving immune system, lipid metabolism, cell, organism and system development. Conclusion: We present a comprehensive result of cattle CNVs at a higher resolution and sensitivity. We identified over 3,000 candidate CNV regions on both Btau_4.0 and UMD3.1, further compared current datasets with previous results, and examined the impacts of genome assemblies on CNV calling. C1 [Hou, Yali; Bickhart, Derek M.; Li, Congjun; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, Bovine Funct Genom Lab, ANRI, BARC East, Beltsville, MD 20705 USA. [Hou, Yali] Chinese Acad Sci, Lab Dis Genom & Individualized Med, Beijing Inst Gen, Beijing 100029, Peoples R China. [Hvinden, Miranda L.; DeNise, Sue] Pfizer Anim Genet, Kalamazoo, MI 49007 USA. [Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Boichard, Didier A.] INRA, Anim Genet & Integrat Biol UMR1313, F-78352 Jouy En Josas, France. [Fritz, Sebastien] UNCEIA Genet Team, F-75595 Paris, France. [Eggen, Andre] Illumina Inc, San Diego, CA 92122 USA. [Wiggans, George R.] USDA ARS, Anim Improvement Programs Lab, ANRI, Beltsville, MD 20705 USA. RP Liu, GE (reprint author), USDA ARS, Bovine Funct Genom Lab, ANRI, BARC East, Beltsville, MD 20705 USA. EM George.Liu@ars.usda.gov OI Van Tassell, Curtis/0000-0002-8416-2087; Bickhart, Derek/0000-0003-2223-9285 FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture Animal Genome Program [2009-65205-05635]; NRI/AFRI from the NIFA [2007-35205-17869, 2011-67015-30183]; USDA-ARS [1265-31000-098-00] FX We thank LABOGENA and other members of the Illumina Bovine HD SNP Consortium for sharing their samples. We would also like to thank R. Anderson, and A. Dimtchev for technical assistance. C.P.V.T. was supported by Agriculture and Food Research Initiative competitive grant no. 2009-65205-05635 from the USDA National Institute of Food and Agriculture Animal Genome Program. G.E.L. was supported by NRI/AFRI grants no. 2007-35205-17869 and 2011-67015-30183 from the USDA CSREES (now NIFA) and Project 1265-31000-098-00 from USDA-ARS. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 42 TC 34 Z9 34 U1 0 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 6 PY 2012 VL 13 AR 376 DI 10.1186/1471-2164-13-376 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 100XF UT WOS:000315737700001 PM 22866901 ER PT J AU Albrecht, E Zhang, DP Mays, AD Saftner, RA Stommel, JR AF Albrecht, Elena Zhang, Dapeng Mays, Anne Deslattes Saftner, Robert A. Stommel, John R. TI Genetic diversity in Capsicum baccatum is significantly influenced by its ecogeographical distribution SO BMC GENETICS LA English DT Article ID CHILI-PEPPERS CAPSICUM; POPULATION-STRUCTURE; SOLANACEAE; TOMATO; DOMESTICATION; DISPERSAL; FRUIT; WILD; L.; EVOLUTIONARY AB Background: The exotic pepper species Capsicum baccatum, also known as the aji or Peruvian hot pepper, is comprised of wild and domesticated botanical forms. The species is a valuable source of new genes useful for improving fruit quality and disease resistance in C. annuum sweet bell and hot chile pepper. However, relatively little research has been conducted to characterize the species, thus limiting its utilization. The structure of genetic diversity in a plant germplasm collection is significantly influenced by its ecogeographical distribution. Together with DNA fingerprints derived from AFLP markers, we evaluated variation in fruit and plant morphology of plants collected across the species native range in South America and evaluated these characters in combination with the unique geography, climate and ecology at different sites where plants originated. Results: The present study mapped the ecogeographic distribution, analyzed the spatial genetic structure, and assessed the relationship between the spatial genetic pattern and the variation of morphological traits in a diverse C. baccatum germplasm collection spanning the species distribution. A combined diversity analysis was carried out on the USDA-ARS C. baccatum germplasm collection using data from GIS, morphological traits and AFLP markers. The results demonstrate that the C. baccatum collection covers wide geographic areas and is adapted to divergent ecological conditions in South America ranging from cool Andean highland to Amazonia rainforest. A high level of morphological diversity was evident in the collection, with fruit weight the leading variable. The fruit weight distribution pattern was compatible to AFLP-based clustering analysis for the collection. A significant spatial structure was observed in the C. baccatum gene pool. Division of the domesticated germplasm into two major regional groups (Western and Eastern) was further supported by the pattern of spatial population structure. Conclusions: The results reported improve our understanding of the combined effects of geography, ecology and human intervention on organization of the C. baccatum genepool. The results will facilitate utilization of C. baccatum for crop improvement and species conservation by providing a framework for efficient germplasm collection management and guidance for future plant acquisitions. C1 [Stommel, John R.] USDA, ARS, Beltsville Agr Res Ctr,Inst Plant Sci, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Albrecht, Elena; Mays, Anne Deslattes] Keygene Inc, Rockville, MD 20850 USA. [Zhang, Dapeng] USDA, ARS, Beltsville Agr Res Ctr,Inst Plant Sci, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Saftner, Robert A.] USDA, ARS, Beltsville Agr Res Ctr,Inst Plant Sci, Food Qual Lab, Beltsville, MD 20705 USA. RP Stommel, JR (reprint author), USDA, ARS, Beltsville Agr Res Ctr,Inst Plant Sci, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov OI Deslattes Mays, Anne/0000-0001-7951-3439 FU Keygene Inc. [58-3 K95-8-1253] FX The authors thank Judith Dumm, Josh Marvel and Eunhee Park for dedicated technical support. This work was partially supported by a Cooperative Research and Development Agreement (58-3 K95-8-1253) with Keygene Inc. AFLP is a registered trademark of Keygene n.v. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 48 TC 6 Z9 8 U1 0 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD AUG 6 PY 2012 VL 13 AR 68 DI 10.1186/1471-2156-13-68 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA 051PJ UT WOS:000312139200001 PM 22866868 ER PT J AU Neill, JD Newcomer, BW Marley, SD Ridpath, JF Givens, MD AF Neill, John D. Newcomer, Benjamin W. Marley, Shonda D. Ridpath, Julia F. Givens, M. Daniel TI Greater numbers of nucleotide substitutions are introduced into the genomic RNA of bovine viral diarrhea virus during acute infections of pregnant cattle than of non-pregnant cattle SO VIROLOGY JOURNAL LA English DT Article DE Persistent infection; Virus genetic diversity; Nucleotide substitutions ID POPULATION DIVERSITY; PERSISTENT INFECTION; APOBEC3C; HOST; CONSEQUENCES; REPLICATION; POLIOVIRUS; ANTIGEN AB Background: Bovine viral diarrhea virus (BVDV) strains circulating in livestock herds show significant sequence variation. Conventional wisdom states that most sequence variation arises during acute infections in response to immune or other environmental pressures. A recent study showed that more nucleotide changes were introduced into the BVDV genomic RNA during the establishment of a single fetal persistent infection than following a series of acute infections of naive cattle. However, it was not known if nucleotide changes were introduce when the virus crossed the placenta and infected the fetus or during the acute infection of the dam. Methods: The sequence of the open reading frame (ORF) from viruses isolated from four acutely infected pregnant heifers following exposure to persistently infected (PI) calves was compared to the sequences of the virus from the progenitor PI calf and the virus from the resulting progeny PI calf to determine when genetic change was introduced. This was compared to genetic change found in viruses isolated from a pregnant PI cow and its PI calf, and in three viruses isolated from acutely infected, non-pregnant cattle exposed to PI calves. Results: Most genetic changes previously identified between the progenitor and progeny PI viruses were in place in the acute phase viruses isolated from the dams six days post-exposure to the progenitor PI calf. Additionally, each progeny PI virus had two to three unique nucleotide substitutions that were introduced in crossing the placenta and infection of the fetus. The nucleotide sequence of two acute phase viruses isolated from steers exposed to PI calves revealed that six and seven nucleotide changes were introduced during the acute infection. The sequence of the BVDV-2 virus isolated from an acute infection of a PI calf (BVDV-1a) co-housed with a BVDV-2 PI calf had ten nucleotides that were different from the progenitor PI virus. Finally, twenty nucleotide changes were identified in the PI virus of a calf born to a PI dam. Conclusions: These results demonstrate that nucleotide changes are introduced into the BVDV infecting pregnant cattle at rates of 2.3 to 8 fold higher then during the acute infection of non-pregnant animals. C1 [Neill, John D.; Ridpath, Julia F.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Newcomer, Benjamin W.; Marley, Shonda D.; Givens, M. Daniel] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. RP Neill, JD (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM john.neill@ars.usda.gov NR 28 TC 5 Z9 5 U1 0 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-422X J9 VIROL J JI Virol. J. PD AUG 6 PY 2012 VL 9 AR 150 DI 10.1186/1743-422X-9-150 PG 8 WC Virology SC Virology GA 032QO UT WOS:000310734200003 PM 22867008 ER PT J AU Ouyang, Y AF Ouyang, Ying TI A potential approach for low flow selection in water resource supply and management SO JOURNAL OF HYDROLOGY LA English DT Article DE 7Q10; Frequent-low approach; Low flow selection; Water resource management AB Low flow selections are essential to water resource management, water supply planning, and watershed ecosystem restoration. In this study, a new approach, namely the frequent-low (FL) approach (or frequent-low index), was developed based on the minimum frequent-low flow or level used in minimum flows and/or levels program in northeast Florida, USA. This FL approach was then compared to the conventional 7Q10 approach for low flow selections prior to its applications, using the USGS flow data from the freshwater environment (Big Sunflower River, Mississippi) as well as from the estuarine environment (St. Johns River, Florida). Unlike the FL approach that is associated with the biological and ecological impacts, the 7Q10 approach could lead to the selections of extremely low flows (e.g., near-zero flows) that may hinder its use for establishing criteria to prevent streams from significant harm to biological and ecological communities. Additionally, the 7Q10 approach could not be used when the period of data records is less than 10 years by definition while this may not the case for the FL approach. Results from both approaches showed that the low flows from the Big Sunflower River and the St. Johns River decreased as time elapsed, demonstrating that these two rivers have become drier during the last several decades with a potential of salted water intrusion to the St. Johns River. Results from the FL approach further revealed that the recurrence probability of low flow increased while the recurrence interval of low flow decreased as time elapsed in both rivers, indicating that low flows occurred more frequent in these rivers as time elapsed. This report suggests that the FL approach, developed in this study, is a useful alternative for low flow selections in addition to the 7Q10 approach. Published by Elsevier B.V. C1 USDA, Forest Serv, Mississippi State, MS 39762 USA. RP Ouyang, Y (reprint author), USDA, Forest Serv, 100 Stone Blvd,Thompson Hall,Room 309, Mississippi State, MS 39762 USA. EM youyang@fs.fed.us NR 21 TC 6 Z9 6 U1 3 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 J9 J HYDROL JI J. Hydrol. PD AUG 6 PY 2012 VL 454 BP 56 EP 63 DI 10.1016/j.jhydrol.2012.05.062 PG 8 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 983YG UT WOS:000307154000005 ER PT J AU Polejaeva, IA Hall, J Meng, QG Zhou, XC Sessions, BR Panter, K Stott, R Rutigliano, HM Davies, CJ Wang, ZD Ranjan, R Dosdall, D MacLeod, R Marrouche, N White, KL AF Polejaeva, Irina A. Hall, Justin Meng, Qinggang Zhou, Xinchang Sessions, Benjamin R. Panter, Kip Stott, Rusty Rutigliano, Heloisa M. Davies, Christopher J. Wang, Zhongde Ranjan, Ravi Dosdall, Derek MacLeod, Rob Marrouche, Nassir White, Kenneth L. TI Development of a Transgenic Goat Model with Cardiac-Specific Overexpression of Transforming Growth Factor-beta 1 to Study the Relationship Between Atrial Fibrosis and Atrial Fibrillation SO CIRCULATION RESEARCH LA English DT Meeting Abstract CT Basic Cardiovascular Sciences Scientific Session CY JUL 23-26, 2012 CL New Orleans, LA DE Transgenic models; Fibrosis; Atrial fibrillation C1 [Polejaeva, Irina A.; Hall, Justin; Meng, Qinggang; Zhou, Xinchang; Sessions, Benjamin R.; Stott, Rusty; Rutigliano, Heloisa M.; Davies, Christopher J.; Wang, Zhongde; White, Kenneth L.] Utah State Univ, Logan, UT 84322 USA. [Panter, Kip] ARS, USDA, Logan, UT USA. [Ranjan, Ravi; Dosdall, Derek; MacLeod, Rob; Marrouche, Nassir] Univ Utah, Salt Lake City, UT USA. RI Rutigliano, Heloisa/J-1933-2016 OI Rutigliano, Heloisa/0000-0003-2807-5007 NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7330 J9 CIRC RES JI Circ.Res. PD AUG 3 PY 2012 VL 111 IS 4 SU S MA 251 PG 1 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA 056QO UT WOS:000312506400239 ER PT J AU Wang, ZY Zarlenga, D Martin, J Abubucker, S Mitreva, M AF Wang, Zhengyuan Zarlenga, Dante Martin, John Abubucker, Sahar Mitreva, Makedonka TI Exploring metazoan evolution through dynamic and holistic changes in protein families and domains SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Proteins; Domains; Evolution; Metazoa; Vertebrates; Arthropods; Nematodes ID NUCLEAR RECEPTOR SUPERFAMILY; ESCHERICHIA-COLI; PLANT PARASITISM; NEMATODE GENOME; GENE FAMILIES; SEQUENCE; DIVERGENCE; INSERTIONS; TREHALOSE; CHICKEN AB Background: Proteins convey the majority of biochemical and cellular activities in organisms. Over the course of evolution, proteins undergo normal sequence mutations as well as large scale mutations involving domain duplication and/or domain shuffling. These events result in the generation of new proteins and protein families. Processes that affect proteome evolution drive species diversity and adaptation. Herein, change over the course of metazoan evolution, as defined by birth/death and duplication/deletion events within protein families and domains, was examined using the proteomes of 9 metazoan and two outgroup species. Results: In studying members of the three major metazoan groups, the vertebrates, arthropods, and nematodes, we found that the number of protein families increased at the majority of lineages over the course of metazoan evolution where the magnitude of these increases was greatest at the lineages leading to mammals. In contrast, the number of protein domains decreased at most lineages and at all terminal lineages. This resulted in a weak correlation between protein family birth and domain birth; however, the correlation between domain birth and domain member duplication was quite strong. These data suggest that domain birth and protein family birth occur via different mechanisms, and that domain shuffling plays a role in the formation of protein families. The ratio of protein family birth to protein domain birth (domain shuffling index) suggests that shuffling had a more demonstrable effect on protein families in nematodes and arthropods than in vertebrates. Through the contrast of high and low domain shuffling indices at the lineages of Trichinella spiralis and Gallus gallus, we propose a link between protein redundancy and evolutionary changes controlled by domain shuffling; however, the speed of adaptation among the different lineages was relatively invariant. Evaluating the functions of protein families that appeared or disappeared at the last common ancestors (LCAs) of the three metazoan clades supports a correlation with organism adaptation. Furthermore, bursts of new protein families and domains in the LCAs of metazoans and vertebrates are consistent with whole genome duplications. Conclusion: Metazoan speciation and adaptation were explored by birth/death and duplication/deletion events among protein families and domains. Our results provide insights into protein evolution and its bearing on metazoan evolution. C1 [Wang, Zhengyuan; Martin, John; Abubucker, Sahar; Mitreva, Makedonka] Washington Univ, Genome Inst, Sch Med, St Louis, MO 63108 USA. [Mitreva, Makedonka] Washington Univ, Div Infect Dis, Sch Med, Dept Med, St Louis, MO 63108 USA. [Zarlenga, Dante] Agr Res Serv, USDA, ANRI, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Mitreva, Makedonka] Washington Univ, Dept Genet, Sch Med, St Louis, MO 63108 USA. RP Mitreva, M (reprint author), Washington Univ, Genome Inst, Sch Med, 4444 Forest Pk Blvd, St Louis, MO 63108 USA. EM mmitreva@genome.wustl.edu FU NIH NIAID [AI081803] FX This work was supported in part be the NIH NIAID grant AI081803 to M.M. NR 80 TC 6 Z9 6 U1 0 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD AUG 3 PY 2012 VL 12 AR 138 DI 10.1186/1471-2148-12-138 PG 17 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 027DG UT WOS:000310331400001 PM 22862991 ER PT J AU Smyth, K Garcia, K Sun, ZF Tuo, WB Xiao, ZG AF Smyth, Kendra Garcia, Karla Sun, Zhifeng Tuo, Wenbin Xiao, Zhengguo TI Repetitive peptide boosting progressively enhances functional memory CTLs SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article DE T cells; Cytotoxicity; Peptide; Memory; Adjuvant; Boosting ID T-CELL MEMORY; EFFECTOR; PHENOTYPE; IMMUNITY; VACCINATION; INDUCTION; VACCINES; DIFFERENTIATION; HETEROGENEITY; EXPRESSION AB Cytotoxic T lymphocytes (CTLs) play a critical role in controlling intracellular pathogens and cancer cells, and induction of memory CTLs holds promise for developing effective vaccines against critical virus infections. However, generating memory CTLs remains a major challenge for conventional vector-based, prime-boost vaccinations. Thus, it is imperative that we explore nonconventional alternatives, such as boosting without vectors. We show here that repetitive intravenous boosting with peptide and adjuvant generates memory CD8 T cells of sufficient quality and quantity to protect against infection in mice. The resulting memory CTLs possess a unique and long-lasting effector memory phenotype, characterized by decreased interferon-gamma but increased granzyme B production. These results are observed in both transgenic and endogenous models. Overall, our findings have important implications for future vaccine development, as they suggest that intravenous peptide boosting with adjuvant following priming can induce long-term functional memory CTLs. (C) 2012 Elsevier Inc. All rights reserved. C1 [Smyth, Kendra; Garcia, Karla; Sun, Zhifeng; Xiao, Zhengguo] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Tuo, Wenbin] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Xiao, ZG (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM xia00028@umd.edu FU National Institutes of Health Grants [R21AI095715A] FX This work was supported by National Institutes of Health Grants R21AI095715A (to X.Z.) and Startup (to X.Z.). NR 38 TC 3 Z9 3 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD AUG 3 PY 2012 VL 424 IS 3 BP 635 EP 640 DI 10.1016/j.bbrc.2012.07.018 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 990GK UT WOS:000307618800047 PM 22809501 ER PT J AU Jang, SI Lillehoj, HS Lee, SH Lee, KW Lillehoj, EP Hong, YH An, DJ Jeong, W Chun, JE Bertrand, F Dupuis, L Deville, S Ben Arous, J AF Jang, Seung I. Lillehoj, Hyun S. Lee, Sung-Hyen Lee, Kyung Woo Lillehoj, Erik P. Hong, Yeong Ho An, Dong-Jun Jeong, Wooseog Chun, Ji-Eun Bertrand, Francois Dupuis, Laurent Deville, Sebastien Ben Arous, Juliette TI Vaccination with Clostridium perfringens recombinant proteins in combination with Montanide (TM) ISA 71 VG adjuvant increases protection against experimental necrotic enteritis in commercial broiler chickens SO VACCINE LA English DT Article DE Chicken; Adjuvant; Necrotic enteritis; Immunity; Cytokine ID ANTIGEN VACCINATION; EIMERIA-ACERVULINA; GENE-EXPRESSION; IMMUNIZATION; INFECTION; RESPONSES; PROMOTES; MAXIMA; TOXIN AB This study was performed to compare four Clostridium perfringens recombinant proteins as vaccine candidates using the Montanide (TM) ISA 71 VG adjuvant in an experimental model of necrotic enteritis. Broiler chickens were immunized subcutaneously with purified clostridial recombinant NetB toxin, pyruvate: ferredoxin oxidoreductase (PFO), alpha-toxin, or elongation factor-Tu (EF-Tu), or with vehicle control, in conjunction with ISA 71 VG, and intestinal lesion scores, body weight gains, NetB toxin and PFO antibody levels, and proinflammatory cytokine and chemokine levels were measured as outcomes of protection following oral co-infection with C perfringens and Eimeria maxima. Birds immunized with all recombinant proteins plus ISA 71 VG showed significantly reduced gut lesions compared with the ISA 71 VG-only group. Birds immunized with NetB toxin or PFO plus ISA 71 VG exhibited significantly increased body weight gains compared with the ISA 71 VG alone group. Greater NetB toxin antibody titers were observed in the NetB/ISA 71 VG group, and greater PFO antibody titers were evident in the PFO/ISA 71 VG group, each compared with the other three vaccine/adjuvant groups. Finally, decreased levels of gene transcripts encoding interleukin-8, tumor necrosis factor superfamily 15, and LPS-induced TNF-alpha factor were observed in the intestinal lymphocytes of chickens immunized with NetB toxin, PFO, alpha-toxin, and/or EF-Tu in the presence of ISA 71 VG compared with ISA 71 VG alone. All parameters evaluated were equal in co-infected chickens given ISA 71 VG alone compared with infected/adjuvant-free birds, indicating that the adjuvant itself did not have a disease protective effect. These results suggest that vaccination with clostridial recombinant proteins, particularly NetB toxin or PFO, in combination with ISA 71 VG enhances protective immunity against experimental necrotic enteritis in broiler chickens. Published by Elsevier Ltd. C1 [Jang, Seung I.; Lillehoj, Hyun S.; Lee, Sung-Hyen; Lee, Kyung Woo] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. [Hong, Yeong Ho] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [An, Dong-Jun; Jeong, Wooseog; Chun, Ji-Eun] Minist Food Agr Forestry & Fisheries, Anim Plant & Fisheries Quarantine & Inspect Agcy, Anyang 430824, Kyunggido, South Korea. [Bertrand, Francois; Dupuis, Laurent; Deville, Sebastien; Ben Arous, Juliette] SEPPIC, F-92800 Puteaux La Defense, France. RP Lillehoj, HS (reprint author), ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS-USDA; Animal Plant & Fisheries Quarantine & Inspection Agency, South Korea; SEPPIC (Puteaux, France); Next-Generation BioGreen 21 Program, Rural Development Administration [PJ008084]; World Class University Program of the Ministry of Education, Science and Technology of South Korea [R33-10013] FX This project was supported, in part, by the Trust agreements established between ARS-USDA and Animal Plant & Fisheries Quarantine & Inspection Agency, South Korea, between ARS-USDA and SEPPIC (Puteaux, France), between ARS-USDA and the Next-Generation BioGreen 21 Program (No. PJ008084), Rural Development Administration, and the World Class University Program (R33-10013) of the Ministry of Education, Science and Technology of South Korea. The authors thank Margie Nichols and Stacy Torreyson for their significant contribution to this research. NR 27 TC 32 Z9 33 U1 1 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD AUG 3 PY 2012 VL 30 IS 36 BP 5401 EP 5406 DI 10.1016/j.vaccine.2012.06.007 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 991KB UT WOS:000307698800017 PM 22713719 ER PT J AU He, LM Chen, JM Pan, YD Birdsey, R Kattge, J AF He, Liming Chen, Jing M. Pan, Yude Birdsey, Richard Kattge, Jens TI Relationships between net primary productivity and forest stand age in U.S. forests SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article ID LEAF-AREA INDEX; CARBON BUDGET MODEL; SOUTHERN UNITED-STATES; FINE-ROOT PRODUCTION; RELATIVE GROWTH-RATE; ELEVATED CO2; WOODY-PLANTS; PHOTOSYNTHETIC CAPACITY; BIOMASS DISTRIBUTION; SATELLITE IMAGERY AB Net primary productivity (NPP) is a key flux in the terrestrial ecosystem carbon balance, as it summarizes the autotrophic input into the system. Forest NPP varies predictably with stand age, and quantitative information on the NPP-age relationship for different regions and forest types is therefore fundamentally important for forest carbon cycle modeling. We used four terms to calculate NPP: annual accumulation of live biomass, annual mortality of aboveground and belowground biomass, foliage turnover to soil, and fine root turnover in soil. For U. S. forests the first two terms can be reliably estimated from the Forest Inventory and Analysis (FIA) data. Although the last two terms make up more than 50% of total NPP, direct estimates of these fluxes are highly uncertain due to limited availability of empirical relationships between aboveground biomass and foliage or fine root biomass. To resolve this problem, we developed a new approach using maps of leaf area index (LAI) and forest age at 1 km resolution to derive LAI-age relationships for 18 major forest type groups in the USA. These relationships were then used to derive foliage turnover estimates using species-specific trait data for leaf specific area and longevity. These turnover estimates were also used to derive the fine root turnover based on reliable relationships between fine root and foliage turnover. This combination of FIA data, remote sensing, and plant trait information allows for the first empirical and reliable NPP-age relationships for different forest types in the USA. The relationships show a general temporal pattern of rapid increase in NPP in the young ages of forest type groups, peak growth in the middle ages, and slow decline in the mature ages. The predicted patterns are influenced by climate conditions and can be affected by forest management. These relationships were further generalized to three major forest biomes for use by continental-scale carbon cycle models in conjunction with remotely sensed land cover types. C1 [He, Liming; Chen, Jing M.] Univ Toronto, Dept Geog, Toronto, ON M5S 3G3, Canada. [He, Liming; Chen, Jing M.] Univ Toronto, Program Planning, Toronto, ON M5S 3G3, Canada. [Pan, Yude; Birdsey, Richard] US Forest Serv, USDA, Newtown Sq, PA USA. [Kattge, Jens] Max Planck Inst Biogeochem, Jena, Germany. RP He, LM (reprint author), Univ Toronto, Dept Geog, 100 St George St,Room 5047, Toronto, ON M5S 3G3, Canada. EM liming.he@gmail.com RI Pan, Yude/F-6145-2015; Kattge, Jens/J-8283-2016; OI Kattge, Jens/0000-0002-1022-8469; He, Liming/0000-0003-4010-6814 FU Global Change Program of the Chinese Ministry of Science and Technology [2010CB950700]; Canadian Foundation for Climate and Atmospheric Sciences; U.S. Forest Service, Northern Research Station's climate change research program; TRY initiative; DIVERSITAS; IGBP; Global Land Project, QUEST; GIS "Climat, Environnement et Societe" France FX The MODIS LAI product was downloaded from ftp://primavera.bu.edu/pub/datasets/. The VCF product (collection 4, version 3) was downloaded from http://landcover.org/data/vcf/. This study was supported by a project (2010CB950700) founded by the Global Change Program of the Chinese Ministry of Science and Technology, the Canadian Foundation for Climate and Atmospheric Sciences, and the U.S. Forest Service, Northern Research Station's climate change research program. The study has been supported by the TRY initiative (http://www.trydb.org). TRY is hosted at the Max Planck Institute for Biogeochemistry, Jena, Germany, and supported by DIVERSITAS, IGBP, the Global Land Project, QUEST and GIS "Climat, Environnement et Societe" France. We are thankful to all the anonymous reviewers for constructive suggestions. NR 108 TC 27 Z9 27 U1 8 U2 106 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD AUG 3 PY 2012 VL 26 AR GB3009 DI 10.1029/2010GB003942 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA 988FA UT WOS:000307473000001 ER PT J AU Bruns, E Carson, M May, G AF Bruns, Emily Carson, Martin May, Georgiana TI Pathogen and host genotype differently affect pathogen fitness through their effects on different life-history stages SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Host-pathogen interactions; Life history; Density-dependence; Fitness; Pathogen reproductive rate; Puccinia coronata; Avena sativa ID GENE-FOR-GENE; WHEAT LEAF RUST; OAT CROWN RUST; LOCAL ADAPTATION; PARASITE INTERACTIONS; NATURAL-POPULATIONS; DISEASE RESISTANCE; PUCCINIA-CORONATA; SEPTORIA-TRITICI; PLANT-PATHOGENS AB Background: Adaptation of pathogens to their hosts depends critically on factors affecting pathogen reproductive rate. While pathogen reproduction is the end result of an intricate interaction between host and pathogen, the relative contributions of host and pathogen genotype to variation in pathogen life history within the host are not well understood. Untangling these contributions allows us to identify traits with sufficient genetic variation for selection to act and to identify mechanisms of coevolution between pathogens and their hosts. We investigated the effects of pathogen and host genotype on three life-history components of pathogen fitness; infection efficiency, latent period, and sporulation capacity, in the oat crown rust fungus, Puccinia coronata f.sp. avenae, as it infects oats (Avena sativa). Results: We show that both pathogen and host genotype significantly affect total spore production but do so through their effects on different life-history stages. Pathogen genotype has the strongest effect on the early stage of infection efficiency, while host genotype most strongly affects the later life-history stages of latent period and sporulation capacity. In addition, host genotype affected the relationship between pathogen density and the later life-history traits of latent period and sporulation capacity. We did not find evidence of pathogen-by-host genotypic (GxG) interactions. Conclusion: Our results illustrate mechanisms by which variation in host populations will affect the evolution of pathogen life history. Results show that different pathogen life-history stages have the potential to respond differently to selection by host or pathogen genotype and suggest mechanisms of antagonistic coevolution. Pathogen populations may adapt to host genotypes through increased infection efficiency while their plant hosts may adapt by limiting the later stages of pathogen growth and spore production within the host. C1 [Bruns, Emily; May, Georgiana] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Bruns, Emily] Univ Minnesota, Grad Program Ecol Evolut & Behav, St Paul, MN 55108 USA. [Carson, Martin] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Bruns, E (reprint author), Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. EM bruns094@umn.edu FU Minnesota Agricultural Experiment Station and Department of Plant Biology at the University of Minnesota [MIN-71-024]; NSF grant [1045608] FX We thank Jerry Ochocki (USDA Cereal Disease Lab) and Julia Beni (undergraduate research assistant) for their help growing and inoculating plants. We also thank Ruth Shaw, Peter Tiffin and two anonymous reviewers for valuable comments on the manuscript. A Hatch Grant (MIN-71-024) from the Minnesota Agricultural Experiment Station and Department of Plant Biology at the University of Minnesota and an NSF grant (1045608) funded the work. NR 69 TC 9 Z9 9 U1 3 U2 46 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD AUG 2 PY 2012 VL 12 AR 135 DI 10.1186/1471-2148-12-135 PG 13 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 027DF UT WOS:000310331300001 PM 22857005 ER PT J AU Srour, A Afzal, AJ Blahut-Beatty, L Hemmati, N Simmonds, DH Li, WB Liu, M Town, CD Sharma, H Arelli, P Lightfoot, DA AF Srour, Ali Afzal, Ahmed J. Blahut-Beatty, Laureen Hemmati, Naghmeh Simmonds, Daina H. Li, Wenbin Liu, Miao Town, Christopher D. Sharma, Hemlata Arelli, Prakash Lightfoot, David A. TI The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses SO BMC GENOMICS LA English DT Article DE Segregation; Pleiotropy; Rhg1/Rfs2; Soybean; Resistance; Soybean cyst nematode (SCN); Sudden death syndrome (SDS); Insect herbivory ID HETERODERA-GLYCINES; FUSARIUM-VIRGULIFORME; RHG1 LOCUS; MOLECULAR CHARACTERIZATION; UNDERLYING RESISTANCE; FUNCTIONAL-ANALYSIS; DISEASE RESISTANCE; ESCHERICHIA-COLI; FIELD-RESISTANCE; GENOMIC ANALYSIS AB Background: Soybean (Glycine max (L. Merr.)) resistance to any population of Heterodera glycines (I.), or Fusarium virguliforme (Akoi, O'Donnell, Homma & Lattanzi) required a functional allele at Rhg1/Rfs2. H. glycines, the soybean cyst nematode (SCN) was an ancient, endemic, pest of soybean whereas F. virguliforme causal agent of sudden death syndrome (SDS), was a recent, regional, pest. This study examined the role of a receptor like kinase (RLK) GmRLK18-1 (gene model Glyma_18_02680 at 1,071 kbp on chromosome 18 of the genome sequence) within the Rhg1/Rfs2 locus in causing resistance to SCN and SDS. Results: A BAC (B73p06) encompassing the Rhg1/Rfs2 locus was sequenced from a resistant cultivar and compared to the sequences of two susceptible cultivars from which 800 SNPs were found. Sequence alignments inferred that the resistance allele was an introgressed region of about 59 kbp at the center of which the GmRLK18-1 was the most polymorphic gene and encoded protein. Analyses were made of plants that were either heterozygous at, or transgenic (and so hemizygous at a new location) with, the resistance allele of GmRLK18-1. Those plants infested with either H. glycines or F. virguliforme showed that the allele for resistance was dominant. In the absence of Rhg4 the GmRLK18-1 was sufficient to confer nearly complete resistance to both root and leaf symptoms of SDS caused by F. virguliforme and provided partial resistance to three different populations of nematodes (mature female cysts were reduced by 30-50%). In the presence of Rhg4 the plants with the transgene were nearly classed as fully resistant to SCN (females reduced to 11% of the susceptible control) as well as SDS. A reduction in the rate of early seedling root development was also shown to be caused by the resistance allele of the GmRLK18-1. Field trials of transgenic plants showed an increase in foliar susceptibility to insect herbivory. Conclusions: The inference that soybean has adapted part of an existing pathogen recognition and defense cascade (H. glycines; SCN and insect herbivory) to a new pathogen (F. virguliforme; SDS) has broad implications for crop improvement. Stable resistance to many pathogens might be achieved by manipulation the genes encoding a small number of pathogen recognition proteins. C1 [Srour, Ali; Afzal, Ahmed J.; Hemmati, Naghmeh; Sharma, Hemlata; Lightfoot, David A.] So Illinois Univ, Dept Mol Biol Microbiol & Biochem, Carbondale, IL 62901 USA. [Srour, Ali; Afzal, Ahmed J.; Lightfoot, David A.] So Illinois Univ, Dept Plant Soil & Agr Syst, Carbondale, IL 62901 USA. [Afzal, Ahmed J.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. [Blahut-Beatty, Laureen; Simmonds, Daina H.] Agr & Agri Food Canada, Ottawa, ON K1A 0C6, Canada. [Li, Wenbin; Liu, Miao; Lightfoot, David A.] Harbin Univ, Minist Educ, Key Lab Soybean Biol Chinese, Harbin, Peoples R China. [Town, Christopher D.] JCVI, Rockville, MD USA. [Sharma, Hemlata] MPUAT, Dept Plant Breeding & Genet, Rajasthan Coll Agr, Udaipur, India. [Arelli, Prakash] USDA, Crop Genet Res Unit, Jackson, TN USA. [Lightfoot, David A.] So Illinois Univ, Gen Core Facility, Ctr Excellence Illinois Soybean Ctr, Carbondale, IL 62901 USA. RP Lightfoot, DA (reprint author), So Illinois Univ, Dept Mol Biol Microbiol & Biochem, Carbondale, IL 62901 USA. EM ga4082@siu.edu OI Lightfoot, David/0000-0002-5725-4381 FU NSF [872635, 0487654] FX The physical map was supported by the NSF under Grant No. 872635 and proteins analysis under Grant No. 0487654. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF. We dedicate this manuscript to the memory of Dr. Oval Myers Jr. NR 71 TC 13 Z9 16 U1 1 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 2 PY 2012 VL 13 AR 368 DI 10.1186/1471-2164-13-368 PG 18 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 008EM UT WOS:000308940200001 PM 22857610 ER PT J AU Swayne, DE AF Swayne, David E. TI The role of vaccines and vaccination in high pathogenicity avian influenza control and eradication SO EXPERT REVIEW OF VACCINES LA English DT Editorial Material DE avian influenza; birds; H5N1; poultry; vaccination ID STRATEGIES; EXPERIENCE; POULTRY; VIRUS; H5N1; IMMUNITY; BIRDS C1 Agr Res Serv, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Swayne, DE (reprint author), Agr Res Serv, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.swayne@ars.usda.gov NR 20 TC 16 Z9 16 U1 0 U2 4 PU EXPERT REVIEWS PI LONDON PA UNITEC HOUSE, 3RD FL, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON N3 1QB, ENGLAND SN 1476-0584 J9 EXPERT REV VACCINES JI Expert Rev. Vaccines PD AUG PY 2012 VL 11 IS 8 BP 877 EP 880 DI 10.1586/ERV.12.60 PG 4 WC Immunology SC Immunology GA 073SX UT WOS:000313763900003 PM 23002966 ER PT J AU Bautista, E Strelkoff, TS Clemmens, AJ AF Bautista, E. Strelkoff, T. S. Clemmens, A. J. TI Improved Surface Volume Estimates for Surface Irrigation Volume-Balance Calculations SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING-ASCE LA English DT Article DE Surface irrigation; Hydraulic models; Estimation; Water surface profiles; Analytical techniques ID BORDER-IRRIGATION; SHAPE FACTOR; ADVANCE; PARAMETERS; MODELS AB This article reviews procedures for estimating surface storage in surface irrigation volume balance calculations. Those procedures are based on the assumption of a power law relationship for flow depth as a function of distance along the stream. The analysis uses zero-inertia simulation and a system of dimensionless variables to examine how the depth profile varies as a function of hydraulic conditions when infiltration is given by the empirical extended Kostiakov equation. Alternatives for approximating the exponent of the depth profile power law (beta) are suggested. The magnitude of the resulting errors relative to zero-inertia model predictions is quantified. Results show that the range of variation for the parameter beta increases with field slope, with increasing advance length relative to the maximum advance distance, and when infiltration rates are relatively constant with time during the irrigation event. Estimating beta as a function of advance distance is most challenging under these conditions. Potentially large errors in the determination of beta do not undermine the proposed procedures when the surface volume represents only a small fraction of the applied volume. Users of volume balance procedures need to be aware of conditions in which uncertain surface volume calculations can lead to potentially large volume balance errors and, thus, in which results need to be interpreted carefully. DOI: 10.1061/(ASCE)IR.1943-4774.0000461. (C) 2012 American Society of Civil Engineers. C1 [Bautista, E.; Strelkoff, T. S.; Clemmens, A. J.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. [Clemmens, A. J.] W Consultants, Tempe, AZ 85284 USA. RP Bautista, E (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85238 USA. EM Eduardo.Bautista@ars.usda.gov; Theodor.Strelkoff@ars.usda.gov; bclemmens@westconsultants.com NR 15 TC 2 Z9 2 U1 2 U2 13 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 J9 J IRRIG DRAIN E-ASCE JI J. Irrig. Drainage Eng-ASCE PD AUG PY 2012 VL 138 IS 8 BP 715 EP 726 DI 10.1061/(ASCE)IR.1943-4774.0000461 PG 12 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA 059PV UT WOS:000312718400002 ER PT J AU Bautista, E Strelkoff, TS Clemmens, AJ AF Bautista, E. Strelkoff, T. S. Clemmens, A. J. TI Errors in Infiltration Calculations in Volume-Balance Models SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING-ASCE LA English DT Article DE Surface irrigation; Infiltration; Simulation models; Hydraulic models; Analytical techniques ID IRRIGATION ADVANCE DATA; SURFACE IRRIGATION; FURROW INFILTRATION; PARAMETERS AB Volume-balance models of surface irrigation calculate the infiltrated volume at a given time as a product of the stream length, upstream infiltration, and shape factors. The best-known expression of this type was derived by combining the Lewis-Milne equation with empirical power-law expressions for infiltration and advance as functions of time. This expression results in systematic errors that are not well understood by users of volume-balance methods. This article examines those errors in furrow irrigation by comparison with infiltrated volumes computed with zero-inertia simulation. The potential for errors is greatest with light soils and where the bottom slope is large enough to produce kinematic flow conditions. An example is presented to show how these errors in a parameter-estimation problem based on a volume balance can be corrected iteratively with the help of zero-inertia simulation. DOI: 10.1061/(ASCE)IR.1943-4774.0000462.(C) 2012 American Society of Civil Engineers. C1 [Bautista, E.; Strelkoff, T. S.; Clemmens, A. J.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. [Clemmens, A. J.] W Consultants Inc, Tempe, AZ 85284 USA. RP Bautista, E (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85238 USA. EM Eduardo.Bautista@ars.usda.gov; Theodor.Strelkoff@ars.usda.gov; bclemmens@westconsultants.com NR 20 TC 1 Z9 1 U1 2 U2 8 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 J9 J IRRIG DRAIN E-ASCE JI J. Irrig. Drainage Eng-ASCE PD AUG PY 2012 VL 138 IS 8 BP 727 EP 735 DI 10.1061/(ASCE)IR.1943-4774.0000462 PG 9 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA 059PV UT WOS:000312718400003 ER PT J AU Holt, GA McIntyre, G Flagg, D Bayer, E Wanjura, JD Pelletier, MG AF Holt, G. A. McIntyre, G. Flagg, D. Bayer, E. Wanjura, J. D. Pelletier, M. G. TI Fungal Mycelium and Cotton Plant Materials in the Manufacture of Biodegradable Molded Packaging Material: Evaluation Study of Select Blends of Cotton Byproducts SO JOURNAL OF BIOBASED MATERIALS AND BIOENERGY LA English DT Article; Proceedings Paper CT 3rd International Conference on Innovative Natural Fibre Composites for Industrial Applications CY OCT 12-14, 2011 CL Sapienza Univ Roma, Rome, ITALY HO Sapienza Univ Roma DE Composite; Cotton; Mycelium; Biobased; Biodegradable ID REINFORCED COMPOSITES; PROXIMATE COMPOSITION; CULTIVATION; CHITOSAN; SINGER AB Since polystyrene is non-biodegradable, a biodegradable material that is eco-friendly is being sought as a substitute for packaging and insulation board consumers. One such process, developed by Ecovative Design, LLC, involves growing fungal species on agricultural biomass to produce an eco-friendly packaging product (EcoCradle (TM)) and insulation panels (Greensulatem (TM)). The objective of this research was to develop and evaluate six blends of processed cotton plant biomass (CPB) materials as a substrate for colonization of selected fungi in the manufacture of molded packaging material. The blends were comprised of processed CPB, cotton seed hulls, starch, and gypsum. The four ingredients were the same mix percentage for all six blends with the particle size of the CPM being the only difference. CPB particles sizes ranged from 0.1 to 51 mm. Tests were conducted to evaluate the physical and mechanical properties of the six CPB blends. Test results revealed blends that met or exceeded like characteristics of extruded polystyrene foam. C1 [Holt, G. A.; Wanjura, J. D.; Pelletier, M. G.] USDA ARS, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. [McIntyre, G.; Flagg, D.; Bayer, E.] Ecovat Design LLC, Green Isl, NY 12183 USA. RP Holt, GA (reprint author), USDA ARS, Cotton Prod & Proc Res Unit, 1604 E FM 1294, Lubbock, TX 79403 USA. EM greg.holt@ars.usda.gov NR 27 TC 5 Z9 5 U1 8 U2 63 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1556-6560 J9 J BIOBASED MATER BIO JI J. Biobased Mater. Bioenergy PD AUG PY 2012 VL 6 IS 4 SI SI BP 431 EP 439 DI 10.1166/jbmb.2012.1241 PG 9 WC Chemistry, Applied; Energy & Fuels; Materials Science, Biomaterials SC Chemistry; Energy & Fuels; Materials Science GA 039QO UT WOS:000311261800012 ER PT J AU Poulose, SM Carey, AN Shukitt-Hale, B AF Poulose, Shibu M. Carey, Amanda N. Shukitt-Hale, Barbara TI Improving brain signaling in aging: could berries be the answer? SO EXPERT REVIEW OF NEUROTHERAPEUTICS LA English DT Editorial Material ID OLDER-ADULTS; BLUEBERRY; MEMORY; SUPPLEMENTATION; FRUIT; NEURODEGENERATION; CONSUMPTION; FLAVONOIDS; COGNITION; KINASE C1 [Shukitt-Hale, Barbara] Tufts Univ, USDA HNRCA, Neurosci & Aging Lab, Boston, MA 02111 USA. [Poulose, Shibu M.; Carey, Amanda N.] Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA HNRCA, Neurosci & Aging Lab, 711 Washington St, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov NR 22 TC 13 Z9 13 U1 3 U2 17 PU EXPERT REVIEWS PI LONDON PA UNITEC HOUSE, 3RD FL, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON N3 1QB, ENGLAND SN 1473-7175 J9 EXPERT REV NEUROTHER JI Expert Rev. Neurother. PD AUG PY 2012 VL 12 IS 8 BP 887 EP 889 DI 10.1586/ERN.12.86 PG 3 WC Clinical Neurology; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA 028FQ UT WOS:000310408600001 PM 23002931 ER PT J AU Pecina-Quintero, V Anaya-Lopez, JL Zamarripa-Colmenero, A Montes-Garcia, N Nunez-Colin, C Solis-Bonilla, JL Aguilar-Rangel, MR Prom, L AF Pecina-Quintero, Victor Luis Anaya-Lopez, Jose Zamarripa-Colmenero, Alfredo Montes-Garcia, Noe Nunez-Colin, Carlos Luis Solis-Bonilla, Jose Rocio Aguilar-Rangel, Maria Prom, Louis TI Genetic diversity of sweet sorghum germplasm in Mexico using AFLP and SSR markers SO PESQUISA AGROPECUARIA BRASILEIRA LA English DT Article DE Sorghum bicolor; biofuels; genetic improvement; molecular marker; rare allele ID BICOLOR L. MOENCH; SIMPLE-SEQUENCE REPEATS; ETHANOL-PRODUCTION; LINKAGE-MAP; COLLECTION; RFLP AB The objective of this work was to evaluate the diversity and genetic relationships between lines and varieties of the sweet sorghum (Sorghum bicolor) germplasm bank of the National Institute for Forestry, Agriculture and Livestock Research, Mexico, using AFLP and SSR markers. The molecular markers revealed robust amplification profiles and were able to differentiate the 41 genotypes of sweet sorghum evaluated. Analysis of the frequency and distribution of polymorphic fragments allowed for the detection of unique (AFLP) and rare (SSR) alleles in several genotypes (RBSS-8, RBSS-9, RBSS-25, RBSS-32, and RBSS-37), indicating that these markers may be associated with a feature that has not yet been determined or may be useful for the identification of these genotypes. The genetic relationships indicated the presence of at least two types of sweet sorghum: a group of modern genotypes used for sugar and biofuel production, and another group consisting of historic and modern genotypes used for the production of syrups. Sweet sorghum genotypes may be used to develop new varieties with higher sugar and juice contents. C1 [Pecina-Quintero, Victor; Luis Anaya-Lopez, Jose; Nunez-Colin, Carlos] INIFAP, Guanajuato 38110, Mexico. [Zamarripa-Colmenero, Alfredo; Luis Solis-Bonilla, Jose] INIFAP, Tuxtla Chico, Chiapas, Mexico. [Montes-Garcia, Noe] INIFAP, Rio Bravo, Tamaulipas, Mexico. [Prom, Louis] ARS, USDA, College Stn, TX USA. RP Pecina-Quintero, V (reprint author), INIFAP, Campo Expt Bajio,Km 6-5,Carretera Celaya San Migu, Guanajuato 38110, Mexico. EM pecina.victor@inifap.gob.mx; anaya.jose@inifap.gob.mx; zamarripa.alfredo@inifap.gob.mx; montes.noe@inifap.gob.mx; nunez.carlos@inifap.gob.mx; solis.joseluis@inifap.gob.mx; rociobacks@hotmail.com; louis.prom@ars.usda.gov OI Nunez-Colin, Carlos Alberto/0000-0002-9912-6097 FU Secretaria de Agricultura Ganaderia, Desarrollo Rural, Pesca y Alimentacion (SAGARPA); SAGARPA-Consejo Nacional de Ciencia y Tecnologia Sectorial Fund; Fondos Mixtos Guanajuto FX To Secretaria de Agricultura Ganaderia, Desarrollo Rural, Pesca y Alimentacion (SAGARPA) for financial support; to SAGARPA-Consejo Nacional de Ciencia y Tecnologia Sectorial Fund and to Fondos Mixtos Guanajuto, for support. NR 34 TC 4 Z9 5 U1 0 U2 7 PU EMPRESA BRASIL PESQ AGROPEC PI BRASILIA DF PA EMBRAPA INFORMACAO TECNOLOGICA, PESQUISA AGROPECUARIA BRASILEIRA - PAB, CAIXA POSTAL 040315, 70770-901 BRASILIA DF, BRAZIL SN 0100-204X J9 PESQUI AGROPECU BRAS JI Pesqui. Agropecu. Bras. PD AUG PY 2012 VL 47 IS 8 BP 1095 EP 1102 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA 024DX UT WOS:000310089400009 ER PT J AU McArthur, RI Zhu, XW Oliver, RE Klindworth, DL Xu, SS Stack, RW Wang, RRC Cai, XW AF McArthur, Rachel I. Zhu, Xianwen Oliver, Rebekah E. Klindworth, Daryl L. Xu, Steven S. Stack, Robert W. Wang, Richard R. -C. Cai, Xiwen TI Homoeology of Thinopyrum junceum and Elymus rectisetus chromosomes to wheat and disease resistance conferred by the Thinopyrum and Elymus chromosomes in wheat SO CHROMOSOME RESEARCH LA English DT Article DE Homoeology; Wheat; Thinopyrum junceum; Elymus rectisetus; Molecular markers; Disease resistance ID FUSARIUM HEAD BLIGHT; MOLECULAR CYTOGENETIC CHARACTERIZATION; ALIEN SPECIES DERIVATIVES; ADDITION LINES; STEM RUST; HOMOEOLOGOUS RECOMBINATION; POLYPLOID WHEAT; RFLP ANALYSIS; LEAF RUST; GENES AB Thirteen common wheat "Chinese Spring" (CS)-Thinopyrum junceum addition lines and three common wheat "Fukuhokomuji"(Fuku)-Elymus rectisetus addition lines were characterized and verified as disomic additions of a Th. junceum or E. rectisetus chromosome in the wheat backgrounds by fluorescent genomic in situ hybridization. Another Fuku-E. rectisetus addition line, A1048, was found to contain multiple segregating E. rectisetus chromosomes. Seven partial CS-Th. junceum amphiploids were identified to combine 12-16 Th. junceum chromosomes with CS wheat chromosomes. The disomic addition lines AJDAj5, 7, 8, 9, and HD3508 were identified to contain a Th. junceum chromosome in homoeologous group 1. Two of them, AJDAj7 and AJDAj9, had the same Th. junceum chromosome. AJDAj2, 3, and 4 contained a Th. junceum chromosome in group 2, HD3505 in group 4, AJDAj6 and AJDAj11 in group 5, and AJDAj1 probably in group 6. The disomic addition lines A1026 and A1057 were identified to carry an E. rectisetus chromosome in group 1 and A1034 in group 5. E. rectisetus chromosomes in groups 1-6 were detected in A1048. The homoeologous group of the Th. junceum chromosome in HD3515 could not be determined in this study. Several Th. junceum and E. rectisetus chromosomes in the addition lines were found to contain genes for resistance to Fusarium head blight, tan spot, Stagonospora nodorum blotch, and stem rust (Ug99 races). Understanding of the homoeology of the Th. junceum and E. rectisetus chromosomes with wheat will facilitate utilization of the favorable genes on these alien chromosomes in wheat improvement. C1 [McArthur, Rachel I.; Zhu, Xianwen; Oliver, Rebekah E.; Cai, Xiwen] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Klindworth, Daryl L.; Xu, Steven S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Stack, Robert W.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Wang, Richard R. -C.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. RP Cai, XW (reprint author), N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM xiwen.cai@ndsu.edu FU US Wheat & Barley Scab Initiative; North Dakota Wheat Commission FX We thank members of the Cai and Xu labs for their help in this research and Drs. Lili Qi and G. Francois Marais for their critical review of this manuscript. Also, we would like to thank Drs. Mark Sorrells and Shahryar Kianian for maintaining and generously providing the RFLP probes for this study. This research was supported by the US Wheat & Barley Scab Initiative and North Dakota Wheat Commission. NR 52 TC 7 Z9 7 U1 0 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-3849 J9 CHROMOSOME RES JI Chromosome Res. PD AUG PY 2012 VL 20 IS 6 BP 699 EP 715 DI 10.1007/s10577-012-9307-y PG 17 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 026YJ UT WOS:000310318700005 PM 22968763 ER PT J AU van Damme, M Bozkurt, TO Cakir, C Schornack, S Sklenar, J Jones, AME Kamoun, S AF van Damme, Mireille Bozkurt, Tolga O. Cakir, Cahid Schornack, Sebastian Sklenar, Jan Jones, Alexandra M. E. Kamoun, Sophien TI The Irish Potato Famine Pathogen Phytophthora infestans Translocates the CRN8 Kinase into Host Plant Cells SO PLOS PATHOGENS LA English DT Article ID TOXOPLASMA-GONDII; PROTEIN-KINASE; III EFFECTORS; TYROSINE-PHOSPHATASE; OOMYCETE EFFECTORS; MOLECULAR-CLONING; VIRULENCE FACTOR; RHOPTRY PROTEIN; INNATE IMMUNITY; RXLR EFFECTORS AB Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector family. The C-terminus of CRN8 localizes to the host nucleus and triggers cell death when the protein is expressed in planta. Cell death induction by CRN8 is dependent on its localization to the plant nucleus, which requires a functional nuclear localization signal (NLS). The C-terminal sequence of CRN8 has similarity to a serine/threonine RD kinase domain. We demonstrated that CRN8 is a functional RD kinase and that its autophosphorylation is dependent on an intact catalytic site. Co-immunoprecipitation experiments revealed that CRN8 forms a dimer or multimer. Heterologous expression of CRN8 in planta resulted in enhanced virulence by P. infestans. In contrast, in planta expression of the dominant-negative CRN8 R469A; D470A resulted in reduced P. infestans infection, further implicating CRN8 in virulence. Overall, our results indicate that similar to animal parasites, plant pathogens also translocate biochemically active kinase effectors inside host cells. C1 [van Damme, Mireille; Bozkurt, Tolga O.; Schornack, Sebastian; Sklenar, Jan; Jones, Alexandra M. E.; Kamoun, Sophien] Sainsbury Lab, Norwich, Norfolk, England. [van Damme, Mireille] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands. [Cakir, Cahid] ARS, USDA, Plant Stress & Germplasm Dev Unit, Lubbock, TX USA. RP van Damme, M (reprint author), Sainsbury Lab, Norwich Res Pk, Norwich, Norfolk, England. EM sophien.kamoun@tsl.ac.uk RI Jones, Alexandra/B-5227-2009; Kamoun, Sophien/B-3529-2009; OI Jones, Alexandra/0000-0003-2571-8708; Kamoun, Sophien/0000-0002-0290-0315; Schornack, Sebastian/0000-0002-7836-5881 FU Gatsby Charitable Foundation; Biotechnology and Biological Sciences Research Council FX The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This work was supported by the Gatsby Charitable Foundation and the Biotechnology and Biological Sciences Research Council. NR 64 TC 28 Z9 28 U1 1 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD AUG PY 2012 VL 8 IS 8 AR e1002875 DI 10.1371/journal.ppat.1002875 PG 12 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA 002TI UT WOS:000308558000049 PM 22927814 ER PT J AU Koch, RL Porter, PM Harbur, MM Abrahamson, MD Wyckhuys, KAG Ragsdale, DW Buckman, K Sezen, Z Heimpel, GE AF Koch, R. L. Porter, P. M. Harbur, M. M. Abrahamson, M. D. Wyckhuys, K. A. G. Ragsdale, D. W. Buckman, K. Sezen, Z. Heimpel, G. E. TI Response of Soybean Insects to an Autumn-Seeded Rye Cover Crop SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Aphis glycines; Cerotoma trifurcata; Empoasca fabae; winter rye; soybean ID POTATO LEAFHOPPER HOMOPTERA; APHIS-GLYCINES MATSUMURA; BEAN LEAF BEETLE; HARMONIA-AXYRIDIS COLEOPTERA; ECONOMIC-INJURY LEVELS; US CORN-BELT; BIOLOGICAL-CONTROL; WEED MANAGEMENT; NATURAL ENEMIES; POPULATION-DYNAMICS AB We evaluated the hypothesis that the use of an autumn-seeded winter rye, Secale cereale L., cover crop in soybean, Glycine max (L.) Merr., could lead to decreased insect pest pressure. We compared insect pest and natural enemy populations in an organic soybean production system with soybeans planted into an autumn-seeded rye cover crop (i.e., soybeans planted in spring into a rye cover crop that had been planted the previous autumn). Small-plot experiments were conducted at two locations in southwestern Minnesota to compare insect populations on soybean planted into fallow ground versus soybean planted into rye that was managed by mowing using three different techniques. Densities of potato leafhopper, Empoasca fabae (Harris), were significantly lower on soybean in all of the rye treatments compared with soybean without rye at both locations. Densities of soybean aphid, Aphis glycines Matsumura, were significantly lower on all of the treatments incorporating rye than the soybean-only treatment at one site, but the suppressive effect of rye depended on the specifics of rye planting at the other site. Densities of bean leaf beetle, Cerotoma trifurcata (Forster), were significantly lower in the rye plots than soybean-only plots at one of the two sites. Foliar-foraging insect predator densities did not differ significantly among treatments at either site. Soybean yields did not differ significantly among treatments at either location although there was a trend for lower yield in two of the rye treatments. A statewide survey also was conducted to compare A. glycines infestations in Minnesota soybean fields with and without a rye cover crop. The proportion of plants infested and infestation ratings were both significantly lower in soybean fields with rye than in those without. C1 [Sezen, Z.; Heimpel, G. E.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Koch, R. L.; Abrahamson, M. D.] Minnesota Dept Agr, St Paul, MN 55155 USA. [Porter, P. M.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Harbur, M. M.] Univ Minnesota, SW Res & Outreach Ctr, Lamberton, MN 56152 USA. [Wyckhuys, K. A. G.] Int Ctr Trop Agr CIAT, Cali 6713, Colombia. [Ragsdale, D. W.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Buckman, K.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Heimpel, GE (reprint author), Univ Minnesota, Dept Entomol, 219 Hodson Hall,1980 Folwell Ave, St Paul, MN 55108 USA. EM heimp001@umn.edu FU Integrated Organics Program; United States Department of Agriculture; Sustainable Agriculture Research and Education Producer Grant FX We thank M. Anderson for field assistance. We are also grateful to D. DeWeerd for allowing us to establish research plots on his farm at Pipestone, MN and the comments of three anonymous reviewers. This work was funded by the Integrated Organics Program, United States Department of Agriculture to GEH, PMP, and DWR, and a Sustainable Agriculture Research and Education Producer Grant awarded to D. DeWeerd. NR 105 TC 9 Z9 9 U1 4 U2 38 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 750 EP 760 DI 10.1603/EN11168 PG 11 WC Entomology SC Entomology GA 019LS UT WOS:000309741700002 ER PT J AU Castrillo, LA Griggs, MH Vandenberg, JD AF Castrillo, Louela A. Griggs, Michael H. Vandenberg, John D. TI Brood Production by Xylosandrus germanus (Coleoptera: Curculionidae) and Growth of Its Fungal Symbiont on Artificial Diet Based on Sawdust of Different Tree Species SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE ambrosia beetle; invasive pest; laboratory rearing; artificial diet ID AMBROSIA BEETLE; SCOLYTIDAE; REPRODUCTION AB The ambrosia beetle Xylosandrus germanus (Blanford) (Coleoptera: Curculionidae) is among the most important exotic pests of orchards and nurseries in the United States. It attacks a wide range of hosts and is difficult to control using conventional insecticides. As part of our studies on the biology and control of X. germanus, we are trying to optimize conditions for rearing beetle progeny to adulthood in the laboratory. In this study we tested the brood production by X. germanus reared on artificial diet based on sawdust from American beech (Fagus americana L.), black walnut (Juglans nigra L.), European buckthorn (Rhamnus cathartica L.), and red oak (Quercus rubra L.). We also tested the growth of different strains of the beetle fungal symbiont, Ambrosiella hartigii Batra, on these sawdust diets. Among the sawdust types tested, we found significantly higher average number of progeny produced on diet made with sawdust from European buckthorn (43.9 +/- 1.6) compared with those made with American beech (29.2 +/- 1.7), black walnut (25.5 +/- 1.9), or red oak (26.3 +/- 1.8). The percentage of females producing brood and the brood sex ratio (females: males) were not affected by sawdust type. Sex ratio, however, was correlated with progeny size, with fewer males produced as the number of progeny increased. In smaller broods the ratio observed was approximate to 4:1; in larger broods it approached 20:1. Growth of the fungal symbiont varied with strain and with sawdust. Comparison of progeny production on sawdust-based diet with symbiont growth in vitro suggests that variables other than speed of symbiont growth may be more critical in optimizing brood production. C1 [Castrillo, Louela A.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Griggs, Michael H.; Vandenberg, John D.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Castrillo, LA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM lac48@cornell.edu FU USDA ARS Floral; Nursery Research Initiative; USDA ARS; Cornell University FX We thank Betsy A. Anderson (USDA ARS, Wooster, OH) for assistance in collecting beetles; Stephen P. Wraight (USDA ARS, Ithaca, NY) for advice on statistical analysis; and Christopher M. Ranger, Michael E. Reding (USDA ARS, Wooster, OH), Peter B. Schultz (Virginia Tech, VA Beach, VA), Jason B. Oliver and Nadeer Youssef (Tennessee State University, McMinnville, TN) for permission to use unpublished data. This work was funded in part by the USDA ARS Floral and Nursery Research Initiative and through an agreement between the USDA ARS and Cornell University (A. Hajek, cooperator). NR 31 TC 4 Z9 4 U1 0 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 822 EP 827 DI 10.1603/EN11251 PG 6 WC Entomology SC Entomology GA 019LS UT WOS:000309741700009 ER PT J AU Prasifka, JR Bradshaw, JD Gray, ME AF Prasifka, J. R. Bradshaw, J. D. Gray, M. E. TI Potential Biomass Reductions to Miscanthus x giganteus by Stem-Boring Caterpillars SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE ethanol; bioenergy; herbivore; pest management; lesser cornstalk borer ID SOUTHWESTERN CORN-BORER; LEPIDOPTERA PYRALIDAE; 1ST REPORT; CRAMBIDAE; MAIZE; CROP; SWITCHGRASS; POPULATIONS; BIOLOGY; DAMAGE AB Injury from stem-boring caterpillars has been observed on the perennial grass Miscanthus x giganteus Greef and Deuter ex Hodkinson and Renvoize in both its native and introduced ranges. Because some species causing stem injury in the United States have not been identified, potential biomass reductions to M. X giganteus were measured using southwestern corn borer, Diatraea grandiosella Dyar (Crambidae), an insect pest of several related crops within the Andropogoneae. Results indicate D. grandiosella is capable of survival on whorl and stem tissue from hatch to 21 d in the laboratory, and field infestations with third instars support adult development, as exuviae were found during dissection of injured tillers. Relative to uninfested controls, M. X giganteus tillers with stem injury yielded 12-30% less dry mass in four infestations over 2009-2010. As in some D. grandiosella hosts, data indicate decreased susceptibility to stem-boring as tillers increase in size or age. Regressions of residuals (observed - predicted mass) for injured M. X giganteus tillers onto the cumulative length of tunnels per tiller also showed significant negative slopes (i.e., decreasing tiller mass with increasing tunnel length). Although D. grandiosella survival appeared low in both laboratory and field trials, results indicate that M. X giganteus productivity could become limited by other stem-boring caterpillars known to attack Andropogoneae, including the following: Elasmopalpus lignosellus (Zeller) (Pyralidae), Diatraea saccharalis (F.) (Pyralidae), and Eoreuma loftini (Dyar) (Crambidae). For perennial grasses grown exclusively for biomass, certain management strategies for stem borers or other pests may be uneconomical or impractical, suggesting long-term investment in breeding for host plant resistance may be needed. C1 [Prasifka, J. R.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. [Bradshaw, J. D.] Univ Nebraska Lincoln, Panhandle Res & Extens Ctr, Scottsbluff, NE USA. [Gray, M. E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Prasifka, JR (reprint author), ARS, No Crop Sci Lab, USDA, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM jarrad.prasifka@ars.usda.gov FU Energy Biosciences Institute FX Research funding was provided by the Energy Biosciences Institute. We appreciate assistance from Drew Schlumpf and Chris Rudisill, who helped with establishment and maintenance of research plots. Bret Hash and Kevin Mazur assisted in artificial infestations of M. X giganteus. NR 33 TC 6 Z9 6 U1 2 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 865 EP 871 DI 10.1603/EN11254 PG 7 WC Entomology SC Entomology GA 019LS UT WOS:000309741700015 ER PT J AU Addesso, KM Mcauslane, HJ Cherry, R AF Addesso, Karla M. Mcauslane, Heather J. Cherry, Ron TI Aggregation Behavior of the Southern Chinch Bug (Hemiptera: Blissidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Blissus insularis Barber; aggregation; pheromones; host-plant volatiles; Y-tube olfactometer ID WING-DIMORPHIC CRICKET; ST-AUGUSTINEGRASS; LYGAEIDAE; HETEROPTERA; RESISTANCE; PHEROMONE; FEMALES; MIRIDAE; ALLOCATION; RESOURCES AB The southern chinch bug, Blissus insularis Barber, forms dense, multigenerational aggregations in St. Augustinegrass lawns leading to grass death from sap feeding. We conducted laboratory bioassays to better understand the signals responsible for the formation and maintenance of southern chinch bug aggregations. In small arena assays, chinch bugs demonstrated a stronger aggregation response over time and aggregated more often on or beneath St. Augustinegrass leaf blades than on or under artificial leaf-like shelters constructed from white or green paper. In Y-tube olfactometer assays, bugs of different age and sex were attracted to volatiles from mixed-sex chinch bug aggregations and showed particular attraction to groups of adult female chinch bugs. Adult males and nymphs were also attracted to adult males. Nymphs were attracted to nymphs and were also more attracted to aggregation volatiles when they could see bugs in the arm of the Y-tube. Adult males were more attracted to short-winged than long-winged adults, while females and nymphs demonstrated no preference. All bugs were attracted to St. Augustinegrass volatiles when presented alone, but only males preferred the odor of grass over odor released from a chinch bug mixed-sex aggregation. When presented with a choice of grass and grass + aggregation volatiles, males preferred the combined treatment. The results of these assays suggest that a complex combination of life stage, sex, as well as plant and insect-derived signals influence chinch bug aggregation behavior. C1 [Addesso, Karla M.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Mcauslane, Heather J.; Cherry, Ron] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Addesso, KM (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600-1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM karla.addesso@ars.usda.gov FU Florida Agricultural Experiment Station FX We thank Laureen Chan and Wendy Gonzales-Canal for their assistance in performing behavioral bioassays, Lucy Skelley for maintaining plant and insect cultures, and Alvin Wilson for collecting and shipping chinch bugs to Gainesville from Belle Glade, FL. This research was supported by the Florida Agricultural Experiment Station. NR 37 TC 2 Z9 2 U1 0 U2 18 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 887 EP 895 DI 10.1603/EN11145 PG 9 WC Entomology SC Entomology GA 019LS UT WOS:000309741700018 ER PT J AU Rao, S Strange, JP AF Rao, Sujaya Strange, James P. TI Bumble Bee (Hymenoptera: Apidae) Foraging Distance and Colony Density Associated With a Late-Season Mass Flowering Crop SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Bombus; microsatellites; pollinators; clover ID BOMBUS-TERRESTRIS HYMENOPTERA; NEST DENSITY; SIBSHIP RECONSTRUCTION; AGRICULTURAL LANDSCAPE; RANGE; POPULATION; DIFFERENTIATION; POLLINATORS; DIVERSITY; FREQUENCY AB Foraging behaviors of bumble bee workers have been examined in natural habitats, whereas agricultural landscapes, which can provide insights on flight distances to fragmented patches of bloom, have received limited attention. In particular, information on worker flight distances to crops blooming several months after nests have been established is invaluable. Here, we examined foraging patterns of Bombus vosnesenskii Radoszkowski in late-season blooming clover in the agricultural-dominated Willamette Valley in Oregon. Workers from 10 fields collected over 2 yr were assigned to full sibling families (colonies) by using eight microsatellite loci. With estimation of numbers of unseen species, we inferred the presence of 189 colonies from 433 bees genotyped in year 1, and 144 from 296 genotyped the next year. Worker foraging distance was estimated to be at least 11.6 km, half the distance between the most remote fields visited by the same colonies. Numbers of nests contributing workers to each field ranged from 15 to 163. Overall, 165 (50%) colonies foraged in two or more fields, and thus used common resources within the landscape. Estimates of average nest densities in the landscape each year ranged from 0.76/km(2) to 22.16/km(2), and highlighted the influences of various study parameters incorporated into the calculation including sample size, distances between sites, and analytical tools used to estimate unsampled individuals. Based on the results, bumble bees can fly long distances, and this could facilitate their survival in fragmented agricultural landscapes. This has important implications for the scale of habitat management in bumble bee conservation programs. C1 [Rao, Sujaya] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Strange, James P.] Utah State Univ, USDA, ARS, Pollinating Insect Res Unit, Logan, UT 84333 USA. RP Rao, S (reprint author), Oregon State Univ, Dept Crop & Soil Sci, 3017 ALS, Corvallis, OR 97331 USA. EM sujaya@oregonstate.edu OI Strange, James/0000-0002-9612-6868 FU Oregon Clover Commission; Western Sustainable Agriculture, Research and Education FX We thank clover seed growers in the Willamette Valley for permission to use their fields for this study. Sarah Maxfield-Taylor, Kathryn Ackerman, and Kimberly Skyrm provided valuable help with field-work, while Joe Kaser constructed the map of field sites. Joyce Knoblett performed PCR and assisted with genotyping. The project was funded by the Oregon Clover Commission and the Western Sustainable Agriculture, Research and Education. NR 52 TC 8 Z9 8 U1 7 U2 74 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 905 EP 915 DI 10.1603/EN11316 PG 11 WC Entomology SC Entomology GA 019LS UT WOS:000309741700020 ER PT J AU Yang, S Duan, JJ Watt, T Abell, KJ Van Driesche, RG AF Yang, Song Duan, Jian J. Watt, Timothy Abell, Kristopher J. Van Driesche, Roy G. TI Responses of an Idiobiont Ectoparasitoid, Spathius galinae (Hymenoptera: Braconidae), to Host Larvae Parasitized by the Koinobiont Endoparasitoid Tetrastichus planipennisi (Hymenoptera: Eulophidae): Implications for Biological Control of Emerald Ash Borer (Coleoptera: Buprestidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Spathius galinae; Tetrastichus planipennisi; interspecific competition; biological control; Agrilus planipennis ID RUSSIAN FAR-EAST; AGRILUS-PLANIPENNIS; NATURAL ENEMIES; INTERSPECIFIC COMPETITION; PARASITOIDS; CHINA; ENCYRTIDAE; DORYCTINAE; HISTORY AB Understanding interspecific competition among insect parasitoids is important in designing classical biological control programs that involve multiple species introductions. Spathius galinae Belokobylskij and Strazenac, a new idiobiont ectoparasitoid from the Russian Far East, currently is being considered for introduction to the United States for biological control of the emerald ash borer, Agrilus planipennis Fairmaire, whereas Tetrastichus planipennisi Yang, a koinobiont endoparasitoid native to China, is another natural enemy that already has been introduced in the same program. In a laboratory study, we examined the potential interactions between these two parasitoids when they compete for the same emerald ash borer host larvae. In multiple-choice assays where healthy emerald ash borer larvae were presented along with emerald ash borer larvae previously parasitized by T. planipennisi at different times, S. galinae attacked host larvae already parasitized by T. planipennisi for up to 4 d, but not 8 d. However, parasitism rates were significantly lower in previously parasitized hosts as compared with healthy host larvae. In no-choice tests where S. galinae females were presented either with healthy emerald ash borer larvae or emerald ash borer larvae parasitized by T. planipennisi at several earlier time points, S. galinae again parasitized significantly more healthy host larvae than T. planipennisi-parasitized larvae. These results suggest that the ectoparasitoid S. galinae can discriminate between healthy host larvae and T. planipennisi-parasitized larvae, and competitive interactions between the two parasitoids are likely to be minimal if coreleased by the emerald ash borer biological control program. C1 [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Yang, Song] SW Forestry Univ, Key Lab Forest Disaster Warning & Control Yunnan, Kunming 650224, Yunnan, Peoples R China. [Watt, Timothy] Univ Delaware, Dept Entomol & Wildlife Biol, Newark, DE 19713 USA. [Abell, Kristopher J.; Van Driesche, Roy G.] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA. RP Duan, JJ (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, 501 S Chapel St, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov FU China Scholarship Council; Yunnan Education Department; Yunnan Provincial Key Subject of Forest Protection FX We thank Mike Vella and Susan Barth (USDA ARS) for rearing of emerald ash borer and parasitoid maintenance. We thank Roger Fuester and Doug Luster (USDA ARS) for comments and revisions to this manuscript and Jinquan Wu (USDA ARS) for suggestions on data analysis. S. Yang is grateful to China Scholarship Council, Yunnan Education Department, and Yunnan Provincial Key Subject of Forest Protection for funding his visit to BIIRU. NR 29 TC 11 Z9 12 U1 1 U2 30 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 925 EP 932 DI 10.1603/EN12072 PG 8 WC Entomology SC Entomology GA 019LS UT WOS:000309741700022 ER PT J AU Keesey, IW Barrett, BA Lin, CH Lerch, RN AF Keesey, Ian W. Barrett, Bruce A. Lin, Chung-Ho Lerch, Robert N. TI Electroantennographic Responses of the Small Chestnut Weevil Curculio sayi (Coleoptera: Curculionidae) to Volatile Organic Compounds Identified From Chestnut Reproductive Plant Tissue SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Curculio; chestnut volatile organic compound profile; solid phase microextraction; gas chromatography-mass spectrometry; electroantennogram ID OLFACTORY RECEPTOR NEURONS; MASS-SPECTROMETRY; ANTHONOMUS-RUBI; BLOSSOM; ATTRACTION; MISSOURI; ODOR; SPME AB The primary insect pest of the developing chestnut industry in the central United States is the small chestnut weevil, Curculio sayi (Gyllenhal), which is a specialist on only Castanea trees. Recent research has shown this insect is attracted to and feeds upon the reproductive tissues of the chestnut tree, including the flowers, burs, and nuts. In this study, the major volatile components emanating from the chestnut's reproductive tissues were sampled using solid phase microextraction and static headspace analysis. In total, 59 compounds from these tissues were separated and identified using GC-MS and authenticated reference standards. The majority of compounds identified from the bur and nut tissues were esters (60.2 and 67.4%, respectively). The majority of compounds identified from catkins were alcohols and benzenoids (53.2 and 19.8%, respectively). A subset of those compounds identified from the chestnut plant tissues was used in electroantennogram testing to determine the insect's electrophysiological response to host-generated volatiles. This study identifies the major components of the volatile profile from several important chestnut plant tissues, and was the first to report the volatile compounds from bur tissue. The identification of the major volatiles emanating from chestnut tissue, as well as the associated insect response, are both critical to the successful utilization of these host-plant volatiles as attractants in the development of a semiochemical-based monitoring trap for C. sayi adults. C1 [Keesey, Ian W.; Barrett, Bruce A.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Lin, Chung-Ho] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Lin, Chung-Ho] Univ Missouri, Ctr Agroforestry, Columbia, MO 65211 USA. [Lerch, Robert N.] Univ Missouri, USDA, ARS Cropping Syst & Water Qual Res, Columbia, MO 65211 USA. RP Barrett, BA (reprint author), Univ Missouri, Div Plant Sci, 1-31 Agr Bldg, Columbia, MO 65211 USA. EM barrettb@missouri.edu FU The Center for Agroforestry at the University of Missouri FX We thank The Center for Agroforestry at the University of Missouri for providing partial funding for this project. We also thank Bettina Coggeshall and Joe Absheer for their technical support, and Terrell Stamps (University of Missouri) for reviewing an earlier version of the manuscript. NR 39 TC 3 Z9 3 U1 0 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 933 EP 940 DI 10.1603/EN12065 PG 8 WC Entomology SC Entomology GA 019LS UT WOS:000309741700023 ER PT J AU Spurgeon, DW Cooper, WR AF Spurgeon, D. W. Cooper, W. R. TI Temperature-Dependent Reproductive Development of Lygus hesperus (Hemiptera: Miridae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE temperature; reproductive development; western tarnished plant bug ID DIFFERENT GENDER; KNIGHT; DIAPAUSE; STATES AB Recent studies to elucidate relationships between the western tarnished plant bug, Lygus hesperus Knight, and injury to cotton (Gossypium spp.) have identified important differences in feeding behaviors among adults of different gender and reproductive states. These findings suggest a need for improved knowledge of L. hesperus temperature-dependent reproductive development. We used nonlinear biophysical development rate models to describe the reproductive development of adult L. hesperus held under constant temperatures from 12.8 to 35.0 degrees C. In addition, abdominal coloration was measured as a nondestructive indicator of reproductive maturity. The nonlinear models indicated most stages of ovary, seminal vesicle, and medial accessory gland development were subject to mild high-temperature inhibition, except for the stage of filled seminal vesicles. Development of filled seminal vesicles was subject to minor low-temperature inhibition and severe high-temperature inhibition. Estimated development times reflected extensive interindividual variation, especially at low temperatures. This variation suggests the opportunity to select for more rapid or more consistent reproductive development under the conditions of laboratory culture. Although presence of a dark aqua coloration of the ventral abdominal cuticle was statistically associated with reproductive maturity, especially in female L. hesperus, color-based predictions of reproductive maturity were often inaccurate for bugs reared at temperatures above or below 26.7 degrees C. Therefore, use of abdominal coloration as an indicator of reproductive development in ecological studies seems inappropriate. The results provide mathematical descriptions of L. hesperus temperature-dependent reproductive development which should facilitate improved planning and interpretation of studies involving manipulation of adult reproductive status. C1 [Spurgeon, D. W.; Cooper, W. R.] ARS, USDA, WICSRU Shafter Cotton Res Stn, Shafter, CA 93263 USA. RP Spurgeon, DW (reprint author), ARS, USDA, WICSRU Shafter Cotton Res Stn, 17053 N Shafter Ave, Shafter, CA 93263 USA. EM dale.spurgeon@ars.usda.gov RI Cooper, William/D-3205-2017 NR 18 TC 8 Z9 8 U1 0 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 941 EP 949 DI 10.1603/EN11250 PG 9 WC Entomology SC Entomology GA 019LS UT WOS:000309741700024 ER PT J AU Miller, NJ Sun, J Sappington, TW AF Miller, Nicholas J. Sun, Jing Sappington, Thomas W. TI High-Throughput Transcriptome Sequencing for SNP and Gene Discovery in a Moth SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Striacosta albicosta; western bean cutworm; single nucleotide polymorphism; second-generation sequencing; transcriptome ID WESTERN BEAN CUTWORM; POLYMORPHIC MICROSATELLITE LOCI; EUROPEAN CORN-BORER; RNA-SEQ DATA; OSTRINIA-NUBILALIS; LEPIDOPTERA-NOCTUIDAE; MOLECULAR-BIOLOGY; CRAMBIDAE; GENOME; DIABROTICA AB The western bean cutworm, Striacosta albicosta (Smith) (Leptidoptera: Noctuidae) is a pest of corn (Zea mays L.) and dry beans that underwent a dramatic range expansion in North America during the 1st decade of the 21st century. Research into the population genetics of this species has been hindered by a lack of genetic markers. The transcriptome of adult male S. albicosta was partially sequenced using Illumina sequencing-by-synthesis. Assembly of the sequence reads yielded 16,847 transcript sequences, of which 6,631 could be assigned a putative function. A search for single nucleotide polymorphisms (SNPs) identified 2,487 candidate SNPs distributed among 1,265 transcripts. A panel of 108 candidate SNPs was selected for empirical testing, of which 68 proved to be assayable polymorphisms that are suitable for population studies. This work provides significant genetic resources for studying S. albicosta and demonstrates the power of applying of second-generation sequencing to previously understudied species. C1 [Miller, Nicholas J.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Sun, Jing] Iowa State Univ, Dept Entomol, Ames, IA USA. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Genet Lab, Ames, IA USA. RP Miller, NJ (reprint author), Univ Nebraska, Dept Entomol, Entomol Hall, Lincoln, NE 68583 USA. EM nmiller4@unl.edu NR 57 TC 3 Z9 4 U1 1 U2 37 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 997 EP 1007 DI 10.1603/EN11216 PG 11 WC Entomology SC Entomology GA 019LS UT WOS:000309741700031 ER PT J AU Zhao, LM Chen, J Jones, WA Oi, DH Drees, BM AF Zhao, Liming Chen, Jian Jones, Walker A. Oi, David H. Drees, Bastian M. TI Molecular Comparisons Suggest Caribbean Crazy Ant From Florida and Rasberry Crazy Ant From Texas (Hymenoptera: Formicidae: Nylanderia) Are the Same Species SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Nylanderia pubens; cDNA; genomic DNA; ecotype; Partrechina pubens ID ACID-BINDING PROTEINS; DIVERSITY; INSECTS; GENOME AB In 2002, a new invasive pest ant in the genus, Nylanderia (formerly Paratrechina), was found in Houston, TX. This invasive ant has been causing significant economic and ecological damage in infested areas. Because of the morphological and behavioral similarities to Nylanderia pubens Forel (Caribbean crazy ant) found in Florida, this ant was named Nylanderia sp. nr. pubens (Rasberry crazy ant). So far, morphometric and phylogenetic analyses have not determined if the two ants are the same or separate species. To determine the relationships between the two populations, a molecular approach was undertaken. Five novel genes with various functions from N. pubens and N. sp. nr. pubens were cloned, sequenced, and identified, including a chemosensory protein (NpCsp), the cyclophilin-like protein (NpClp), the fatty acid binding protein (NpFabp), the ferritin 2-like protein (NpFlp), and an odorant binding protein (NpObp). The cDNA sequences of NpCsp, NpFabp, NpFlp, and NpObp, shared 100% identity between N. sp. nr. pubens and N. pubens. The cDNA of NpClp shared 99% identity, with the only difference at the nucleotide position 358. Comparisons of four partial genomic DNA sequences from Caribbean and Rasberry crazy ants indicated 100% identity for a 710-bp partial genomic DNA sequence of cytochrome oxidase subunit I gene, 99% identity for a 774 bp, and a 452-bp partial genomic DNA sequence of NpFabp and NpObp containing noncoding regions, and 100% identity for a 289 bp partial genomic DNA sequence of NpCsp containing only coding region. This study showed that N. sp. nr. pubens in Texas is the same, or at most an intraspecific variant or ecotype of the species in Florida. C1 [Zhao, Liming; Chen, Jian; Jones, Walker A.] ARS, Biol Control Pests Res Unit, Natl Biol Control Lab, USDA, Stoneville, MS 38776 USA. [Oi, David H.] ARS, Imported Fire Ant & Household Insect Res Unit, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Drees, Bastian M.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. RP Zhao, LM (reprint author), ARS, Biol Control Pests Res Unit, Natl Biol Control Lab, USDA, 59 Lee Rd, Stoneville, MS 38776 USA. EM liming.zhao@ars.usda.gov NR 24 TC 6 Z9 7 U1 5 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 1008 EP 1018 DI 10.1603/EN11287 PG 11 WC Entomology SC Entomology GA 019LS UT WOS:000309741700032 ER PT J AU Swisher, KD Munyaneza, JE Crosslin, JM AF Swisher, Kylie D. Munyaneza, Joseph E. Crosslin, James M. TI High Resolution Melting Analysis of the Cytochrome Oxidase I Gene Identifies Three Haplotypes of the Potato Psyllid in the United States SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE potato; zebra chip; Bactericera cockerelli; liberibacter; psyllid haplotypes ID CANDIDATUS LIBERIBACTER SOLANACEARUM; ZEBRA CHIP DISEASE; BACTERICERA-COCKERELLI SULC; 1ST REPORT; REAL-TIME; PCR; PLANTS; TRANSMISSION; POPULATIONS; ASSOCIATION AB The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a vector of the bacterium "Candidatus Liberibacter solanacearum," the putative causal agent of potato zebra chip disease that has seriously affected the potato industry in the Central and Southwestern United States for the past decade. The 2011 potato growing season saw the first report of zebra chip disease in Washington, Oregon, and Idaho; however, B. cockerelli has been recorded in this region every season at least for the past 7 yr. Studies were conducted to determine the relationship between psyllids collected from the Pacific Northwest potatoes in 2011 and those from the Southwestern and Central United States. High resolution melting analysis of the B. cockerelli mitochondrial Cytochrome C Oxidase subunit I-like gene was conducted on over 450 psyllids collected from numerous locations across the Central and Western United States. Results suggest that at least three potato psyllid haplotypes exist in the United States, correlating to the Central, Western, and Northwestern United States geographical regions. The high resolution melting analysis results were subsequently supported by DNA sequencing data. C1 [Swisher, Kylie D.; Crosslin, James M.] ARS, USDA, Prosser, WA 99350 USA. [Munyaneza, Joseph E.] ARS, USDA, Wapato, WA 98951 USA. RP Crosslin, JM (reprint author), ARS, USDA, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM jim.crosslin@ars.usda.gov FU USDA-SCRI Project [2009-51181-20176] FX We thank John Goolsby, Andy Jensen, John Trumble, Gary Franc, and Sylvia Rondon for kindly providing us with the potato psyllids used in this study. Additionally, we thank Eric Krohn, Mary Sue Roster, and Launa Hamlin for their technical assistance. Financial support for this research was provided by the USDA-SCRI Project # 2009-51181-20176. NR 25 TC 43 Z9 46 U1 0 U2 10 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2012 VL 41 IS 4 BP 1019 EP 1028 DI 10.1603/EN12066 PG 10 WC Entomology SC Entomology GA 019LS UT WOS:000309741700033 ER PT J AU Pan, ZL Bingol, G Brandl, MT McHugh, TH AF Pan, Zhongli Bingol, Gokhan Brandl, Maria T. McHugh, Tara H. TI Review of Current Technologies for Reduction of Salmonella Populations on Almonds SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Review DE Nuts; Salmonellosis; Foodborne diseases; Pasteurization; Contamination; Outbreaks ID ENTERITIDIS PHAGE TYPE-30; ENTERICA SEROVAR ENTERITIDIS; ESCHERICHIA-COLI O157-H7; CHLORINE DIOXIDE GAS; RAW ALMONDS; INOCULATED ALMONDS; SURFACE PASTEURIZATION; INACTIVATION KINETICS; PROPYLENE-OXIDE; SURVIVAL AB After the 2001 and 2004 Salmonellosis outbreaks that were associated with raw almonds, ensuring the microbial safety of almonds by treating them to achieve a minimum 4-log reduction of Salmonella population became mandatory in California, the world's largest almond producer. In this paper, we summarize potential pathways of microbial contamination during almond production and manufacturing. Furthermore, we review sanitization technologies, including chemical, thermal, and non-thermal methods, as well as proprietary systems with regard to microbial and sensory quality of almonds and compare various aspects of their use during almond processing. C1 [Pan, Zhongli; Bingol, Gokhan; McHugh, Tara H.] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Brandl, Maria T.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Pan, ZL (reprint author), ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM Zhongli.Pan@ars.usda.gov NR 84 TC 9 Z9 9 U1 6 U2 55 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD AUG PY 2012 VL 5 IS 6 BP 2046 EP 2057 DI 10.1007/s11947-012-0789-6 PG 12 WC Food Science & Technology SC Food Science & Technology GA 021FC UT WOS:000309869700002 ER PT J AU Garcia, RA Rosentrater, KA AF Garcia, Rafael A. Rosentrater, Kurt A. TI Fractionation of Distillers Dried Grains with Solubles (DDGS) Through a Narrowing of Particle Size Distribution Followed by Aspiration SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE DDGS; Fractionation; Protein shift; Aspiration; Particle size distribution ID AIR CLASSIFICATION; COLOR PROPERTIES; CORN; FIBER; SEPARATION; QUALITY; WHEAT AB Distiller's dried grains with solubles (DDGS) may have more value and utility if they can be separated into high protein and high fiber fractions. A variety of such separation processes have been proposed; two of the most promising processes involve three screening and three air classification unit operations. In the present study, an alternative process involving fewer unit operations is demonstrated. DDGS are subjected to a single screening, and the oversize particles are processed in a mill setup so that it narrows the particle size distribution of the oversize fraction. The milled DDGS is then processed in an air classification device known as an aspirator, which separates it into high and low terminal velocity fractions. The combination of the undersize fraction and the low terminal velocity fraction were substantially enriched in protein. The separation achieved by this process compares favorably to other reported processes while being less complex. C1 [Garcia, Rafael A.] ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Rosentrater, Kurt A.] ARS, N Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. RP Garcia, RA (reprint author), ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Rafael.Garcia@ars.usda.gov RI Garcia, Rafael/D-2796-2009; OI Garcia, Rafael/0000-0002-5452-3929; Rosentrater, Kurt/0000-0003-0131-7037 NR 20 TC 1 Z9 1 U1 1 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD AUG PY 2012 VL 5 IS 6 BP 2623 EP 2629 DI 10.1007/s11947-011-0637-0 PG 7 WC Food Science & Technology SC Food Science & Technology GA 021FC UT WOS:000309869700060 ER PT J AU Hallacoglu, B Sassaroli, A Wysocki, M Guerrero-Berroa, E Beeri, MS Haroutunian, V Shaul, M Rosenberg, IH Troen, AM Fantini, S AF Hallacoglu, Bertan Sassaroli, Angelo Wysocki, Michael Guerrero-Berroa, Elizabeth Beeri, Michal Schnaider Haroutunian, Vahram Shaul, Merav Rosenberg, Irwin H. Troen, Aron M. Fantini, Sergio TI Absolute measurement of cerebral optical coefficients, hemoglobin concentration and oxygen saturation in old and young adults with near-infrared spectroscopy SO JOURNAL OF BIOMEDICAL OPTICS LA English DT Article DE biomedical optics; biophotonics; optical devices; optical properties ID TIME-RESOLVED REFLECTANCE; BLOOD-VOLUME; NONINVASIVE MEASUREMENT; PREFRONTAL CORTEX; BRAIN ACTIVATION; AGE; TISSUES; SCATTERING; GENDER; WATER AB We present near-infrared spectroscopy measurement of absolute cerebral hemoglobin concentration and saturation in a large sample of 36 healthy elderly (mean age, 85 +/- 6 years) and 19 young adults (mean age, 28 +/- 4 years). Non-invasive measurements were obtained on the forehead using a commercially available multi-distance frequency-domain system and analyzed using a diffusion theory model for a semi-infinite, homogeneous medium with semi-infinite boundary conditions. Our study included repeat measurements, taken five months apart, on 16 elderly volunteers that demonstrate intra-subject reproducibility of the absolute measurements with cross-correlation coefficients of 0.9 for absorption coefficient (mu(a)), oxy-hemoglobin concentration ([HbO(2)]), and total hemoglobin concentration ([HbT]), 0.7 for deoxy-hemoglobin concentration ([Hb]), 0.8 for hemoglobin oxygen saturation (StO(2)), and 0.7 for reduced scattering coefficient (mu'(s)). We found significant differences between the two age groups. Compared to young subjects, elderly subjects had lower cerebral [HbO(2)], [Hb], [HbT], and StO(2) by 10 +/- 4 mu M, 4 +/- 3 mu M, 14 +/- 5 mu M, and 6% +/- 5%, respectively. Our results demonstrate the reliability and robustness of multi-distance near-infrared spectroscopy measurements based on a homogeneous model in the human forehead on a large sample of human subjects. Absolute, non-invasive optical measurements on the brain, such as those presented here, can significantly advance the development of NIRS technology as a tool for monitoring resting/basal cerebral perfusion, hemodynamics, oxygenation, and metabolism. 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.8.081406] C1 [Hallacoglu, Bertan; Sassaroli, Angelo; Fantini, Sergio] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA. [Wysocki, Michael; Guerrero-Berroa, Elizabeth; Beeri, Michal Schnaider; Haroutunian, Vahram] Mt Sinai Sch Med, New York, NY 10029 USA. [Shaul, Merav; Rosenberg, Irwin H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Troen, Aron M.] Hebrew Univ Jerusalem, Inst Biochem Food Sci & Nutr, IL-76100 Rehovot, Israel. RP Hallacoglu, B (reprint author), Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA. EM bertan.hallacoglu@tufts.edu RI Hallacoglu, Bertan/K-4175-2012 FU Unilever USA; Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts; U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707]; NIH [R03-MH093846, P01-AG02219] FX This research was supported by a strategic research agreement between Unilever USA and the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts, and by the U.S. Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. We also acknowledge support from NIH Grants R03-MH093846 and P01-AG02219. NR 35 TC 13 Z9 13 U1 1 U2 16 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1083-3668 J9 J BIOMED OPT JI J. Biomed. Opt. PD AUG PY 2012 VL 17 IS 8 AR 081406 DI 10.1117/1.JBO.17.8.081406 PG 8 WC Biochemical Research Methods; Optics; Radiology, Nuclear Medicine & Medical Imaging SC Biochemistry & Molecular Biology; Optics; Radiology, Nuclear Medicine & Medical Imaging GA 018WF UT WOS:000309696800016 PM 23224167 ER PT J AU Witzgall, P Proffit, M Rozpedowska, E Becher, PG Andreadis, S Coracini, M Lindblom, TUT Ream, LJ Hagman, A Bengtsson, M Kurtzman, CP Piskur, J Knight, A AF Witzgall, Peter Proffit, Magali Rozpedowska, Elzbieta Becher, Paul G. Andreadis, Stefanos Coracini, Miryan Lindblom, Tobias U. T. Ream, Lee J. Hagman, Arne Bengtsson, Marie Kurtzman, Cletus P. Piskur, Jure Knight, Alan TI "This is not an Apple"-Yeast Mutualism in Codling Moth SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Plant-insect-microbe-interaction; Mutualism; Herbivory; Chemical communication; Semiochemicals; Tortricidae; Lepidoptera ID CYDIA-POMONELLA; EVOLUTIONARY DIVERSIFICATION; LEPIDOPTERA-TORTRICIDAE; SACCHAROMYCES COMPLEX; RHAGOLETIS FLIES; PLANT VOLATILES; HOST SHIFTS; ACETIC-ACID; SP-NOV.; FRUIT AB The larva of codling moth Cydia pomonella (Tortricidae, Lepidoptera) is known as the worm in the apple, mining the fruit for food. We here show that codling moth larvae are closely associated with yeasts of the genus Metschnikowia. Yeast is an essential part of the larval diet and further promotes larval survival by reducing the incidence of fungal infestations in the apple. Larval feeding, on the other hand, enables yeast proliferation on unripe fruit. Chemical, physiological and behavioral analyses demonstrate that codling moth senses and responds to yeast aroma. Female moths are attracted to fermenting yeast and lay more eggs on yeast-inoculated than on yeast-free apples. An olfactory response to yeast volatiles strongly suggests a contributing role of yeast in host finding, in addition to plant volatiles. Codling moth is a widely studied insect of worldwide economic importance, and it is noteworthy that its association with yeasts has gone unnoticed. Tripartite relationships between moths, plants, and microorganisms may, accordingly, be more widespread than previously thought. It, therefore, is important to study the impact of microorganisms on host plant ecology and their contribution to the signals that mediate host plant finding and recognition. A better comprehension of host volatile signatures also will facilitate further development of semiochemicals for sustainable insect control. C1 [Witzgall, Peter; Proffit, Magali; Rozpedowska, Elzbieta; Becher, Paul G.; Andreadis, Stefanos; Lindblom, Tobias U. T.; Bengtsson, Marie] Swedish Univ Agr Sci, Chem Ecol Grp, S-23053 Alnarp, Sweden. [Hagman, Arne; Piskur, Jure] Lund Univ, Dept Cell & Organism Biol, S-22362 Lund, Sweden. [Kurtzman, Cletus P.] USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. [Ream, Lee J.; Knight, Alan] USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Bengtsson, Marie] Curitiba Univ, Dept Chem, Curitiba, Parana, Brazil. RP Witzgall, P (reprint author), Swedish Univ Agr Sci, Chem Ecol Grp, S-23053 Alnarp, Sweden. EM peter.witzgall@phero.net RI Hagman, Arne/K-8984-2012; Witzgall, Peter/N-6429-2013; OI Witzgall, Peter/0000-0002-4697-3380; Lindblom, Tobias/0000-0002-5826-8822 FU Plant Link (Swedish University of Agricultural Sciences); Plant Link (Lund University); Plant Link (Skane Regional Council); Linnaeus environment "Insect Chemical Ecology, Ethology, and Evolution" IC-E3 (Formas, SLU) FX This study was supported by Plant Link (Swedish University of Agricultural Sciences, Lund University and Skane Regional Council) and the Linnaeus environment "Insect Chemical Ecology, Ethology, and Evolution" IC-E3 (Formas, SLU). Christie J. Robnett is acknowledged for providing technical assistance. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 69 TC 27 Z9 29 U1 4 U2 65 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD AUG PY 2012 VL 38 IS 8 BP 949 EP 957 DI 10.1007/s10886-012-0158-y PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 015WU UT WOS:000309477900001 PM 22797850 ER PT J AU Hudak, AT Strand, EK Vierling, LA Byrne, JC Eitel, JUH Martinuzzi, S Falkowski, MJ AF Hudak, Andrew T. Strand, Eva K. Vierling, Lee A. Byrne, John C. Eitel, Jan U. H. Martinuzzi, Sebastian Falkowski, Michael J. TI Quantifying aboveground forest carbon pools and fluxes from repeat LiDAR surveys SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Discrete return LiDAR; Multi-temporal; Aboveground carbon; Mixed conifer forest; Random forest algorithm; Imputation; Biomass change; Carbon Measuring Reporting and Verification (MRV) ID NEAREST-NEIGHBOR IMPUTATION; DISCRETE-RETURN LIDAR; GROWTH; INVENTORY; SCALE; ACCURACY; BIOMASS; HEIGHT; MODEL; AREA AB Sound forest policy and management decisions to mitigate rising atmospheric CO2 depend upon accurate methodologies to quantify forest carbon pools and fluxes over large tracts of land. LiDAR remote sensing is a rapidly evolving technology for quantifying aboveground biomass and thereby carbon pools: however, little work has evaluated the efficacy of repeat LiDAR measures for spatially monitoring aboveground carbon pools through time. Our study objective was therefore to evaluate the use of discrete return airborne LiDAR for quantifying biomass change and carbon flux from repeat field and LiDAR surveys. We collected LiDAR data in 2003 and 2009 across similar to 20,000 ha of an actively managed, mixed conifer forest landscape in northern Idaho. The Random Forest machine learning algorithm was used to impute aboveground biomass pools of trees, saplings, shrubs, herbaceous plants, coarse and fine woody debris, litter, and duff using field-based forest inventory data and metrics derived from the LiDAR collections. Separate predictive tree aboveground biomass models were developed from the 2003 and 2009 field and LiDAR data, and biomass change was estimated at the plot, pixel, and landscape levels by subtracting 2003 predictions from 2009 predictions. Traditional stand exam data were used to independently validate 2003 and 2009 tree aboveground biomass predictions and tree aboveground biomass change estimates at the stand level. Over this 6-year period, we found a mean increase in tree aboveground biomass due to forest growth across the non-harvested portions of 4.1 Mg/ha/yr. We found that 26.3% of the landscape had been harvested during this time period which outweighed growth at the landscape level, resulting in a net tree aboveground biomass change of -5.7 Mg/ha/yr. and -2.3 Mg/ha/yr in total aboveground carbon, summed across all the aboveground biomass pools. Change in aboveground biomass was related to forest successional status: younger stands gained two- to three-fold less biomass than did more mature stands. This result suggests that even the most mature forest stands are valuable carbon sinks, and implies that forest management decisions that include longer harvest rotation cycles are likely to favor higher levels of aboveground carbon storage in this system. A 30-fold difference in LiDAR sampling density between the 2003 and 2009 collections did not affect plot-scale biomass estimation. These results suggest that repeat LiDAR surveys are useful for accurately quantifying high resolution, spatially explicit biomass and carbon dynamics in conifer forests. Published by Elsevier Inc. C1 [Hudak, Andrew T.; Byrne, John C.] US Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. [Strand, Eva K.; Vierling, Lee A.; Eitel, Jan U. H.] Univ Idaho, Moscow, ID 83843 USA. [Eitel, Jan U. H.] Univ Idaho, McCall Outdoor Sci Sch, Mccall, ID USA. [Martinuzzi, Sebastian] Univ Wisconsin, Madison, WI USA. [Falkowski, Michael J.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. RP Hudak, AT (reprint author), US Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. EM ahudak@fs.fed.us RI Vierling, Lee/E-6428-2010; Beckley, Matthew/D-4547-2013 OI Vierling, Lee/0000-0001-5344-1983; FU Department of Energy (DOE) Big Sky C Sequestration Partnership; Montana State University; Washington State University; U.S. Forest Service Rocky Mountain Research Station [08-JV-11221633-159] FX Primary funding to the University of Idaho for this study was provided by the Department of Energy (DOE) Big Sky C Sequestration Partnership with Montana State and Washington State Universities, with supplemental funding from the U.S. Forest Service Rocky Mountain Research Station through Joint Venture Agreement 08-JV-11221633-159. Cliff Todd, Sean Taylor, Brendon Newman, and John Kyle Parker-Mcglynn did the bulk of the 2009 fieldwork. We thank Linda Tedrow and Patrick Adam for LiDAR processing help, and David Brown and Steven Mulkey for helpful discussions. We thank Halli Hemingway from Bennett Lumber Products, Inc., Brant Steigers and Rob Taylor from Potlatch Forest Holdings, Inc., and Brian Austin from the University of Idaho Experimental Forest, for providing stand exam data used for stand-level validation. Finally, we thank Ralph Dubayah and two anonymous reviewers for their helpful suggestions on an earlier draft of the manuscript. NR 82 TC 66 Z9 67 U1 5 U2 82 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2012 VL 123 BP 25 EP 40 DI 10.1016/j.rse.2012.02.023 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 016CX UT WOS:000309496000003 ER PT J AU Goward, SN Chander, G Pagnutti, M Marx, A Ryan, R Thomas, N Tetrault, R AF Goward, S. N. Chander, G. Pagnutti, M. Marx, A. Ryan, R. Thomas, N. Tetrault, R. TI Complementarity of ResourceSat-1 AWiFS and Landsat TM/ETM+ sensors SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE ResourceSat-1 AWiFS; Landsat Thematic Mapper and Thematic; Mapper Plus; Geometry; Radiometry; BRDF; Agriculture; Forests; CEOS MODRES constellation ID BIDIRECTIONAL REFLECTANCE; TIME-SERIES; PERFORMANCE; CALIBRATION; RETRIEVAL; CANOPIES; DATASET; HISTORY; ALBEDO; BRDF AB Considerable interest has been given to forming an international collaboration to develop a virtual moderate spatial resolution land observation constellation through aggregation of data sets from comparable national observatories such as the US Landsat, the Indian ResourceSat and related systems. This study explores the complementarity of India's ResourceSat-1 Advanced Wide Field Sensor (AWiFS) with the Landsat 5 Thematic Mapper (TM) and Landsat 7 Enhanced Thematic Mapper Plus (ETM+). The analysis focuses on the comparative radiometry, geometry, and spectral properties of the two sensors. Two applied assessments of these data are also explored to examine the strengths and limitations of these alternate sources of moderate resolution land imagery with specific application domains. There are significant technical differences in these imaging systems including spectral band response, pixel dimensions, swath width, and radiometric resolution which produce differences in observation data sets. None of these differences was found to strongly limit comparable analyses in agricultural and forestry applications. Overall, we found that the AWiFS and Landsat TM/ETM+ imagery are comparable and in some ways complementary, particularly with respect to temporal repeat frequency. We have found that there are limits to our understanding of the AWiFS performance, for example, multi-camera design and stability of radiometric calibration over time, that leave some uncertainty that has been better addressed for Landsat through the Image Assessment System and related cross-sensor calibration studies. Such work still needs to be undertaken for AWiFS and similar observatories that may play roles in the Global Earth Observation System of Systems Land Surface Imaging Constellation. (c) 2012 Elsevier Inc. All rights reserved. C1 [Goward, S. N.; Marx, A.; Thomas, N.] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Chander, G.] SGT Inc, USGS, Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. [Pagnutti, M.; Ryan, R.] I2R Corp, Stennis Space Ctr, MS 39529 USA. [Tetrault, R.] USDA, Washington, DC 20250 USA. RP Goward, SN (reprint author), Univ Maryland, Dept Geog, College Pk, MD 20742 USA. EM sgoward@umd.edu FU NASA Land-Cover; Land-Use Change (LCLUC) [NNX09AR41G]; US Geological Survey [08HQCN0005]; NASA [NNX09AV47G, NNG05GE55G]; USDA Data Archive FX This work was supported by the NASA Land-Cover and Land-Use Change (LCLUC) Grant NNX09AR41G (Pagnutti and Ryan). The SGT, Inc., work (Chander) was performed under the US Geological Survey contract 08HQCN0005. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. The UMD work (Goward, Marx and Thomas) was supported under NASA grants NNX09AV47G (Land Use and Land Cover) and NNG05GE55G (Applied Sciences). The support of the USDA Data Archive is much appreciated in accessing the AWiFS imagery. NR 34 TC 14 Z9 15 U1 0 U2 17 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2012 VL 123 BP 41 EP 56 DI 10.1016/j.rse.2012.03.002 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 016CX UT WOS:000309496000004 ER PT J AU Pacheco, JM Tucker, M Hartwig, E Bishop, E Arzt, J Rodriguez, LL AF Pacheco, Juan M. Tucker, Meghan Hartwig, Ethan Bishop, Elizabeth Arzt, Jonathan Rodriguez, Luis L. TI Direct contact transmission of three different foot-and-mouth disease virus strains in swine demonstrates important strain-specific differences SO VETERINARY JOURNAL LA English DT Article DE FMDV; Swine; Direct contact; Transmission; Pathogenesis ID EARLY PATHOGENESIS; NATURAL AEROSOLS; INTERFERON-ALPHA; VACCINATED PIGS; FMD VIRUS; INFECTION; QUANTIFICATION; COMBINATION; PROTECTION; CHALLENGE AB A novel direct contact transmission model for the study of foot-and-mouth disease virus (FMDV) infection of swine was utilized to investigate transmission characteristics of three FMDV strains belonging to serotypes A, O and Asia1. Each strain demonstrated distinct transmission characteristics and required different exposure times to achieve successful contact transmission. While a 4 h exposure was sufficient for strain A24 Cruzeiro (A24Cru), both O1 Manisa and Asia1 Shamir transmission required 18 h or more. Viral excretion levels from donors (for all three strains) and virus present in room air (for A24Cru and O1 Manisa) were evaluated and associated with clinical signs and observed transmission pattern. Although all directly inoculated donor animals showed acute FMD, A24Cru had the highest levels of viral shedding in saliva and nasal swabs followed by O1 Manisa and Asia1 Shamir. Virus levels in room air were higher and were detected longer for A24Cru than for O1 Manisa. These results provide direct evidence for important strain-specific variation in transmission characteristics and emphasize the need for thorough evaluation of different FMDV viral strains using a well defined contact transmission methodology. This information is critical for vaccine and biotherapeutic efficacy testing, pathogenesis and disease modeling of FMDV transmission. Published by Elsevier Ltd. C1 [Pacheco, Juan M.; Tucker, Meghan; Hartwig, Ethan; Bishop, Elizabeth; Arzt, Jonathan; Rodriguez, Luis L.] USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Tucker, Meghan] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN 37831 USA. RP Pacheco, JM (reprint author), USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM juan.pacheco@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU Plum Island Animal Disease Center Research Participation Program fellowship; Department of Homeland Security [60-1940-7-047]; USDA ARS-CRIS [1940-32000-052-00D] FX We want to thank the animal care takers at PIADC for their patience and help collecting the data. M.T. was the recipient of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. This research was funded in part by an interagency agreement with the Department of Homeland Security (#60-1940-7-047) and by USDA ARS-CRIS 1940-32000-052-00D. We thank Drs. Tam Garland, John Vitko and Elizabeth George from DHS and Dr. Michael Farrell from MRI for providing air sampling equipment. NR 31 TC 14 Z9 14 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1090-0233 EI 1532-2971 J9 VET J JI Vet. J. PD AUG PY 2012 VL 193 IS 2 BP 456 EP 463 DI 10.1016/j.tvjl.2012.01.012 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 013LK UT WOS:000309306700029 PM 22342891 ER PT J AU Ashby, RD Solaiman, DKY Strahan, GD Zhu, CJ Tappel, RC Nomura, CT AF Ashby, Richard D. Solaiman, Daniel K. Y. Strahan, Gary D. Zhu, Chengjun Tappel, Ryan C. Nomura, Christopher T. TI Glycerine and levulinic acid: Renewable co-substrates for the fermentative synthesis of short-chain poly(hydroxyalkanoate) biopolymers SO BIORESOURCE TECHNOLOGY LA English DT Article DE Glycerine; Levulinic acid; Short-chain polyhydroxyalkanoate; Physical properties; Pseudomonas oleovorans ID RALSTONIA-EUTROPHA; POLY(3-HYDROXYBUTYRATE) PRODUCTION; POLYHYDROXYALKANOIC ACIDS; PRODUCT STREAM; WASTE GLYCEROL; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE); BIOSYNTHESIS; COPOLYESTERS; POLYESTERS; CONVERSION AB Glycerine (a biodiesel co-product) and levulinic acid (a pulp and paper co-product) were used as co-substrates for the fermentative synthesis of short-chain polyhydroxyalkanoate (sc-PHA) biopolymers with tunable monomer and molecular weight characteristics. Pseudomonas oleovorans NRRL B-14682 utilized glycerine alone to produce poly(3-hydroxybutyrate) (PHB). When levulinic acid was added to the media at shake-flask scale in concentrations <= 0.6 wt.%, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V) copolymers were produced with 3-HV contents ranging from 37 to 97 mol%; a glycerine:levulinic acid ratio of 0.2%:0.8% (w/v) resulted in poly(3-hydroxyvalerate) (PHV). Ten-liter batch fermentations using glycerine:levulinic acid ratios of 1%:0, 0.75%:0.25%, 0.5%:0.5% and 0.25%:0.75% (w/v) resulted in PHB, P(73%-3HB-co-27%-3HV), P(30%-3HB-co-70%-3HV) and PHV with increasing number average molecular weights (x 10(3) g/mol) of 328, 511, 728 and 1330, respectively, owing to glycerine-based chain termination. These results provide a novel means by which glycerine and levulinic acid can be used collectively to produce an array of distinct sc-PHA biopolymers. Published by Elsevier Ltd. C1 [Ashby, Richard D.; Solaiman, Daniel K. Y.; Strahan, Gary D.] USDA, Eastern Reg Res Ctr, ARS, Wyndmoor, PA 19038 USA. [Zhu, Chengjun; Tappel, Ryan C.; Nomura, Christopher T.] SUNY Coll Environm Sci & Forestry SUNY ESF, Dept Chem, Syracuse, NY 13210 USA. RP Ashby, RD (reprint author), USDA, Eastern Reg Res Ctr, ARS, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Rick.Ashby@ars.usda.gov OI Nomura, Christopher/0000-0001-6981-5679 NR 35 TC 17 Z9 17 U1 1 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD AUG PY 2012 VL 118 BP 272 EP 280 DI 10.1016/j.biortech.2012.05.092 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 985JE UT WOS:000307259600038 PM 22705534 ER PT J AU Derner, JD Briske, DD Polley, HW AF Derner, Justin D. Briske, David D. Polley, H. Wayne TI Tiller organization within the tussock grass Schizachyrium scoparium: a field assessment of competition-cooperation tradeoffs SO BOTANY-BOTANIQUE LA English DT Article DE bunchgrass; integrated physiological unit; little bluestem; physiological integration; resource sharing; sectoriality ID PHYSIOLOGICAL INTEGRATION; INTRACLONAL REGULATION; PERENNIAL GRASSES; CLONAL PLANTS; BUD BANKS; GROWTH; BUNCHGRASS; PATTERNS; FORM; SIZE AB Tussock grasses are characterized by a compact spatial arrangement of tillers that contributes to intense intratussock competition. This investigation was designed to directly assess the magnitude of competition among autonomous subunits of tillers within individual tussocks (i.e., integrated physiological units) to further define the mechanisms of tiller organization within this successful growth form. Experimentally constructed tussocks of Schizachyrium scoparium (Michx. Nash) were grown in the field in central Texas, USA, for 2 years with 1, 4, 8, or 12 autonomous subunits to span the range observed in naturally occurring local populations. Increasing numbers of subunits per tussock did not affect tiller density or the mean mass of individual vegetative or reproductive tillers, but it did intensify intratussock competition as evidenced by a large reduction in total tiller mass and number per subunit. This pattern of tiller organization is indicative of a division of labor within the tussock that is manifested as a tradeoff between competition among autonomous subunits and cooperation among tillers within these physiologically integrated subunits. We conclude that an increasing number of autonomous subunits associated with tussock basal expansion contributes to coarse-scale resource preemption and competitive ability, while resource sharing among tillers within subunits supports new tiller establishment and growth within this highly competitive microenvironment. C1 [Derner, Justin D.] USDA Agr Res Serv, High Plains Grasslands Res Stn, Cheyenne, WY 82009 USA. [Briske, David D.] Texas A&M Univ, College Stn, TX 77843 USA. [Polley, H. Wayne] USDA Agr Res Serv, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Derner, JD (reprint author), USDA Agr Res Serv, High Plains Grasslands Res Stn, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. EM Justin.Derner@ars.usda.gov FU USDA-Agricultural Research Service FX The authors thank K. Tiner, H. Harland, B. Kramer, and A. Lopez for collecting vegetation data; M. Mortenson for conducting statistical analyses and preparing figures; and T. Monaco, J. Hendrickson, and two anonymous reviewers for constructive comments. Funding provided by the USDA-Agricultural Research Service. NR 50 TC 6 Z9 6 U1 1 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD AUG PY 2012 VL 90 IS 8 BP 669 EP 677 DI 10.1139/B2012-025 PG 9 WC Plant Sciences SC Plant Sciences GA 999XZ UT WOS:000308350300003 ER PT J AU Bruckart, WL Eskandari, FM Berner, DK Aime, MC AF Bruckart, W. L., III Eskandari, F. M. Berner, D. K. Aime, M. C. TI Comparison of Puccinia acroptili from Eurasia and the USA (vol 90, pg 465, 2012) SO BOTANY-BOTANIQUE LA English DT Correction C1 [Bruckart, W. L., III; Eskandari, F. M.; Berner, D. K.] Agr Res Serv, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Aime, M. C.] Agr Res Serv, USDA, Systemat Bot & Mycol Lab, Beltsville, MD 20705 USA. RP Bruckart, WL (reprint author), Agr Res Serv, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM william.bruckart@ars.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 2 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD AUG PY 2012 VL 90 IS 8 BP 780 EP 780 DI 10.1139/B2012-076 PG 1 WC Plant Sciences SC Plant Sciences GA 999XZ UT WOS:000308350300014 ER PT J AU Shao, DY Atungulu, GG Pan, ZL Yue, TL Zhang, A Li, X AF Shao, Dongyan Atungulu, Griffiths G. Pan, Zhongli Yue, Tianli Zhang, Ang Li, Xuan TI Study of Optimal Extraction Conditions for Achieving High Yield and Antioxidant Activity of Tomato Seed Oil SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antioxidant activity; extraction; kinetics; 2nd order model; tomato seed oil ID VEGETABLE-OILS; BY-PRODUCTS; OLIVE OIL; KINETICS; CAKE AB Value of tomato seed has not been fully recognized. The objectives of this research were to establish suitable processing conditions for extracting oil from tomato seed by using solvent, determine the impact of processing conditions on yield and antioxidant activity of extracted oil, and elucidate kinetics of the oil extraction process. Four processing parameters, including time, temperature, solvent-to-solid ratio and particle size were studied. A second order model was established to describe the oil extraction process. Based on the results, increasing temperature, solvent-to-solid ratio, and extraction time increased oil yield. In contrast, larger particle size reduced the oil yield. The recommended oil extraction conditions were 8 min of extraction time at temperature of 25 degrees C, solvent-to-solids ratio of 5/1 (v/w) and particle size of 0.38 mm, which gave oil yield of 20.32% with recovery rate of 78.56%. The DPPH scavenging activity of extracted oil was not significantly affected by the extraction parameters. The inhibitory concentration (IC50) of tomato seed oil was 8.67 mg/mL which was notably low compared to most vegetable oils. A 2nd order model successfully described the kinetics of tomato oil extraction process and parameters of extraction kinetics including initial extraction rate (h), equilibrium concentration of oil (Cs), and the extraction rate constant (k) could be precisely predicted with R2 of at least 0.957. Practical Application: The study revealed that tomato seed which is typically treated as a low value byproduct of tomato processing has great potential in producing oil with high antioxidant capability. The impact of processing conditions including time, temperature, solvent-to-solid ratio and particle size on yield, and antioxidant activity of extracted tomato seed oil are reported. Optimal conditions and models which describe the extraction process are recommended. The information is vital for determining the extraction processing conditions for industrial production of high quality tomato seed oil. C1 [Shao, Dongyan; Atungulu, Griffiths G.; Pan, Zhongli; Zhang, Ang; Li, Xuan] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Shao, Dongyan; Yue, Tianli] NW A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Pan, Zhongli] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Zhang, Ang] NW A&F Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China. RP Pan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, 1 Shields Ave, Davis, CA 95616 USA. EM zhongli.pan@ars.usda.gov RI Li, Xuan/J-5786-2015 NR 27 TC 4 Z9 4 U1 4 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2012 VL 77 IS 8 BP E202 EP E208 DI 10.1111/j.1750-3841.2012.02804.x PG 7 WC Food Science & Technology SC Food Science & Technology GA 986ZI UT WOS:000307385800017 PM 22809373 ER PT J AU Sheen, S Huang, LH Sommers, C AF Sheen, Shiowshuh Huang, Lihan Sommers, Christopher TI Survival of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. on Catfish Fillets Exposed to Microwave Heating in a Continuous Mode SO JOURNAL OF FOOD SCIENCE LA English DT Article DE E; coli O157:H7; fish fillets; L; monocytogenes; microwave; Salmonella; survival ID BEEF FRANKFURTERS; PASTEURIZATION; INACTIVATION; FOODS; COOKING; SAFETY; OVEN AB Microwave (MW) heating using continuous power output with feedback control and a modified ingredient formulation may provide better and consistent cooking of foods. Currently, household units with build-in inverter power supply units are available. These new generation MW ovens provide continuous, adjustable output and cooking, in contrast to the traditional rectifier-based ovens that rely on the onoff mechanism for control. This study attempted to apply a feedback power control (termed as modified or smart MW oven) and phosphate treatment to further improve heating uniformity and enhance food quality and safety. Listeria monocytogenes (Lm, 4-strain cocktail), Escherichia coli O157:H7 (Ec, 5-strain cocktail), and Salmonella spp. (Sal, 6-strain cocktail), surface inoculated onto catfish fillets (75 x 100 x 15 mm; weight 110 g), were heated using the modified MW oven to study the inactivation of the pathogens. The sensitivity of these 3 bacteria to MW heating was in the order of Ec (most), Lm, and Sal (least). Greater than 4 to 5 log CFU reductions of Ec, Lm, or Sal counts on catfish fillet surfaces were inactivated within 2 min of 1250 W MW heating, where the fillet surface temperature increased from 10 to 20 degrees C to 80 to 90 degrees C. MW heating caused degradation of catfish fillet texture, which was noticeable as early as 10 to 15 s after the heating started, as evidenced by bumping sounds. Bumping can be significantly reduced by soaking fillets in phosphate solution. However, the results may need verification if applied in different MW ovens and/or with foods positioned away the geometric oven center. This study successfully demonstrated the feasibility of applying MW energy to eliminate foodborne pathogens on fish fillets. Practical Application: The results demonstrated in this report with the smart microwave oven design may enhance microwaveable food safety and quality, and therefore promote the microwaveable food business. C1 [Sheen, Shiowshuh; Sommers, Christopher] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Huang, Lihan] ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov NR 24 TC 2 Z9 2 U1 1 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2012 VL 77 IS 8 BP E209 EP E214 DI 10.1111/j.1750-3841.2012.02817.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 986ZI UT WOS:000307385800018 PM 22860585 ER PT J AU Singh, M Kim, S Liu, SX AF Singh, Mukti Kim, Sanghoon Liu, Sean X. TI Effect of Purified Oat ss-Glucan on Fermentation of Set-Style Yogurt Mix* SO JOURNAL OF FOOD SCIENCE LA English DT Article DE aggregation; ss-glucan; fermentation; fortification; gelling; morphology; viscosity; yogurt ID SIZE-EXCLUSION CHROMATOGRAPHY; MOLECULAR-WEIGHT DISTRIBUTION; CEREAL BETA-GLUCANS; RHEOLOGICAL PROPERTIES; NONFAT YOGURT; DIETARY FIBER; PLAIN YOGURT; MILK; FAT; PROTEIN AB Effect of oat beta-glucan on the fermentation of set-style yogurt was investigated by incorporating 0%, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% of purified oat beta-glucan into the yogurt mix. It was found that levels up to 0.3% resulted in yogurts with quality characteristics similar to the control yogurt. Higher levels of beta-glucan however retarded the fermentation process with noticeable difference in the characteristics of the yogurt. Examination of the morphologies of yogurt with and without beta-glucan revealed that beta-glucan formed aggregates with casein micelle and did not form phase-separated domains. This research demonstrated that beta-glucan could be added to yogurt up to 0.3%, which meets the nutrient guidelines, to have added nutritional benefits. Practical Application: Yogurt is known for its beneficial effects on human health and nutrition. Yogurt production and consumption is increasing in the United States every year. However, it is lacking in beta-glucans, which are recognized for their nutritional importance as functional bioactive ingredients. The main objective was to develop and characterize low-fat yogurts with added beta-glucan. This research demonstrated that beta-glucan could be added to yogurt up to 0.3%, which meets the nutrient guidelines for added nutritional benefits, without affecting the characteristics of yogurt significantly. This study will benefit the dairy industry by generating new products offering healthy alternatives. C1 [Singh, Mukti; Liu, Sean X.] ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Kim, Sanghoon] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Singh, M (reprint author), ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Mukti.Singh@ars.usda.gov NR 35 TC 1 Z9 1 U1 1 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2012 VL 77 IS 8 BP E195 EP E201 DI 10.1111/j.1750-3841.2012.02828.x PG 7 WC Food Science & Technology SC Food Science & Technology GA 986ZI UT WOS:000307385800016 PM 22860584 ER PT J AU Hoberg, EP Abrams, A Pilitt, PA Jenkins, EJ AF Hoberg, Eric P. Abrams, Arthur Pilitt, Patricia A. Jenkins, Emily J. TI DISCOVERY AND DESCRIPTION OF A NEW TRICHOSTRONGYLOID SPECIES (NEMATODA: OSTERTAGIINAE), ABOMASAL PARASITES IN MOUNTAIN GOAT, OREAMNOS AMERICANUS, FROM THE WESTERN CORDILLERA OF NORTH AMERICA SO JOURNAL OF PARASITOLOGY LA English DT Article ID SUB-SAHARAN AFRICA; CUTICULAR RIDGE-PATTERNS; MARSHALLAGIA-MARSHALLI; HISTORICAL BIOGEOGRAPHY; OVIBOS-MOSCHATUS; BIGHORN SHEEP; SOUTH DAKOTA; LOPEZ-NEYRA; GEN. NOV; RUMINANTS AB Marshallagia lichtenfelsi sp. n. is a dimorphic ostertaeline nematode occurring in the abomasum of mountain goats, Oreamnos americanus, from the Western Cordillera of North America. Major and minor morphotype males and females are characterized and distinguished relative to the morphologically similar Marshallagia marshallagia Marshallagia occidentalls from North America and Marshallagin dentispicalaris, along with other congeners, from the Palearctic region. The configuration of the convoluted and irregular synlophe in the cervical region of males and females of M. lichtenfelsi is apparently unique, contrasting with a continuous and parallel system of ridges among those species of Marshallagia, including M. marshallil M.. accidentalis, which have been evaluated. Specimens of M.. lichtenfelsi are further defined by the rectangular form of the accessory bursal membrane (width > length) in the major morphotype and by the trapezoidal Sjoberg's organ in the minor morphotype, in addition to specific attributes of the spicules and spicule tips. We regard 12 species, including the proposed new taxon, to be valid. Primary diagnostic characters are reviewed for Marshallagia and a framework is presented for standardization of future descriptions incorporating the synlophe in males and females and the structure of the spicules and genital cone in major and minor morphotype males. The center of diversity for species of Marshallagia is the mountain steppe region of central Eurasia where 11 species (including the Holarctic M. marshalli) are recognized in association with Caprini. Rupicaprini, and Antelopinae; only 2 species occur in the Nearctic. In this assemblage, M. lichtenfelsi is endemic to North America and limited in host distribution to mountain goats. An intricate history for refugial isolation and population fragmentation demonstrated for mountain goats and wild sheep indicates the potential for considerable cryptic diversity for Marshallagia and other nematodes. Shifting patterns of contact and sympatry among assemblages of ungulates during the Pleistocene are consistent with geographic and host colonization as a process involved in diversification of these parasites. C1 [Hoberg, Eric P.; Abrams, Arthur; Pilitt, Patricia A.; Jenkins, Emily J.] ARS, US Natl Parasite Collect & Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Hoberg, EP (reprint author), ARS, US Natl Parasite Collect & Anim Parasit Dis Lab, USDA, BARC E,Bldg 1180,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Eric.Hoberg@ars.usda.gov FU National Science Foundation [DEB 0196095, 0415668]; U.S. National Parasite Collection FX The type host specimen of Oreamnos americanus from the Ospika River was made available for necropsy and parasitological examination by Helen Schwantje, Regional Biologist, Government of British Columbia. We thank Catherine Soos from the Veterinary Diagnostic Laboratory, Western College of Veterinary Medicine, University of Saskatchewan, and the Canadian Cooperative Wildlife Health Center, University of Saskatchewan for conducting the necropsy and for access to nematode parasites recovered by E.P.H. and E.J.J. Some specimens of Marshallagia spp. from Eurasia were kindly made available from the Central Helminthological Museum, Russian Academy of Sciences, by Academician Sergei Bessanov. We are indebted to Wayne Thompson of the National Agricultural Library, USDA, Beltsville, MD for assistance in procuring certain of the Russian and Chinese literature dealing with Marshallagia. Our access to the Chinese literature was further facilitated by Xiao Shu-Hua (retired) from the Institute of Parasitic Diseases, Chinese CDC, and by John Hawdon from George Washington University. We are grateful for additional assistance from Lihua Xiao, Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control, Atlanta, and Monica Santin-Duran of the Environmental Microbial and Food Safety Laboratory, ARS, Beltsville Research Center in searching for Chinese descriptions of Marshallagia. We thank Chris Pooley from the Soybean Genomics and Improvement Laboratory, and the Electron and Confocal Microscopy Unit, ARS, Beltsville Research Center, for configuring and for the limit digital plates of images for M. lichtenfelsi. This is a contribution of the Beringian Coevolution Project, defining the diversity and distribution of high-latitude assemblages of hosts and parasites, supported in part by the National Science Foundation through grants (DEB 0196095 and 0415668) to J. A. Cook (Museum of Southwestern Biology, University of New Mexico) and E.P.H. at the U.S. National Parasite Collection. NR 95 TC 4 Z9 4 U1 3 U2 13 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 EI 1937-2345 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2012 VL 98 IS 4 BP 817 EP 846 DI 10.1645/GE-3047.1 PG 30 WC Parasitology SC Parasitology GA 991DH UT WOS:000307681200020 PM 22316219 ER PT J AU Subar, AF Kirkpatrick, SI Mittl, B Zimmerman, TP Thompson, FE Bingley, C Willis, G Islam, NG Baranowski, T McNutt, S Potischman, N AF Subar, Amy F. Kirkpatrick, Sharon I. Mittl, Beth Zimmerman, Thea Palmer Thompson, Frances E. Bingley, Christopher Willis, Gordon Islam, Noemi G. Baranowski, Tom McNutt, Suzanne Potischman, Nancy TI The Automated Self-Administered 24-Hour Dietary Recall (ASA24): A Resource for Researchers, Clinicians, and Educators from the National Cancer Institute SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE 24-hour dietary recall; Dietary assessment ID BIOMARKER; ACCURACY C1 [Subar, Amy F.] NCI, Div Canc Control & Populat Sci, Appl Res Program, Risk Factor Monitoring & Methods Branch, Bethesda, MD 20892 USA. [Mittl, Beth; Bingley, Christopher] WESTAT Corp, Rockville, MD 20850 USA. [Zimmerman, Thea Palmer] WESTAT Corp, Cleveland, OH USA. [Islam, Noemi G.; Baranowski, Tom] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. [McNutt, Suzanne] WESTAT Corp, Salt Lake City, UT USA. RP Subar, AF (reprint author), NCI, Div Canc Control & Populat Sci, Appl Res Program, Risk Factor Monitoring & Methods Branch, 6130 Execut Blvd,EPN 4005, Bethesda, MD 20892 USA. EM subara@mail.nih.gov OI Kirkpatrick, Sharon/0000-0001-9896-5975; Baranowski, Tom/0000-0002-0653-2222 FU National Institutes of Health Institutes and Offices [ASA24]; National Cancer Institute; National Heart, Lung, and Blood Institute; National Institute for Child Health and Human Development; National Institute for Diabetes and Digestive and Kidney Diseases; Office of Dietary Supplements FX National Institutes of Health Institutes and Offices providing contract funding for ASA24: National Cancer Institute, National Heart, Lung, and Blood Institute, National Institute for Child Health and Human Development, National Institute for Diabetes and Digestive and Kidney Diseases, and Office of Dietary Supplements. NR 12 TC 86 Z9 86 U1 1 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD AUG PY 2012 VL 112 IS 8 BP 1134 EP 1137 DI 10.1016/j.jand.2012.04.016 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 986CJ UT WOS:000307318100004 PM 22704899 ER PT J AU Beasley, JC Beatty, WS Atwood, TC Johnson, SR Rhodes, OE AF Beasley, James C. Beatty, William S. Atwood, Todd C. Johnson, Shylo R. Rhodes, Olin E., Jr. TI A comparison of methods for estimating raccoon abundance: Implications for disease vaccination programs SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE density; mammal; mesopredator; Procyon lotor; rabies; raccoon; resource selection function; vaccine ID WHITE-TAILED DEER; ORAL RABIES VACCINATION; BOVINE TUBERCULOSIS; NORTHERN INDIANA; POPULATION-DENSITY; PUBLIC-HEALTH; WILDLIFE; PREVALENCE; ATTRIBUTES; SELECTION AB Accurate estimates of demographic parameters are critical to the management of wildlife populations, including management programs focused on controlling the spread of zoonotic diseases. Rabies managers in the United States Department of Agriculture (USDA) have applied a simple raccoon (Procyon lotor) abundance index (RAI) based on cumulative catch of unique raccoons per unit area to determine vaccine-bait distribution densities. This approach was designed to allow for both the collection of biological samples and to index raccoon abundance to determine bait densities for oral rabies programs. However, post-baiting surveillance data indicate that, on average, only 30% of raccoons sampled have vaccine induced rabies antibody titers, suggesting that bait densities may not be well calibrated to raccoon densities. We trapped raccoons using both capture-mark-recapture (CMR) and the standard RAI to evaluate the accuracy of the current index-based methodology for estimating raccoon density. We then developed a resource selection function from spatial data collected from radio-collared raccoons to standardize trap placement within the existing RAI protocol, and evaluated the performance of this modified RAI approach relative to CMR for estimating raccoon population size. Both abundance and density estimates derived using the RAI consistently underestimated raccoon population sizes compared with CMR methods. Similarly, although the use of resource selection models to inform trap placement appeared to improve the accuracy of the RAI, the effectiveness of this method was inconsistent because of an inability to account for variance in detection probabilities. Despite the logistical advantages of using indices to estimate population parameters to determine vaccine bait distribution densities, our results suggest that adjustments may be necessary to more accurately quantify raccoon abundance, which should improve the effectiveness of rabies management in the United States. In particular, estimates of detection probabilities are needed to more precisely quantify abundance estimates and ensure appropriate vaccine coverage rates. (c) 2012 The Wildlife Society. C1 [Beasley, James C.; Beatty, William S.; Rhodes, Olin E., Jr.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Atwood, Todd C.; Johnson, Shylo R.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Beasley, JC (reprint author), Purdue Univ, Dept Forestry & Nat Resources, 195 Marsteller St, W Lafayette, IN 47907 USA. EM beasley@purdue.edu OI Beatty, William/0000-0003-0013-3113 FU USDA-APHIS-WS National Rabies Management program; USDA-APHIS-WS National Wildlife Research Center; Department of Forestry and Natural Resources at Purdue University FX This study would not have been possible without the cooperation of the Indiana Department of Natural Resources, as well as the landowners who permitted us access to their land. We also are grateful to the numerous field technicians who assisted with the collection of radio-telemetry and mark-recapture data. We thank D. Slate, R. Chipman, and T. Algeo for providing information on the National Rabies Management Program raccoon abundance index. Funding for this research was provided by the USDA-APHIS-WS National Rabies Management program, USDA-APHIS-WS National Wildlife Research Center, and the Department of Forestry and Natural Resources at Purdue University. NR 56 TC 7 Z9 7 U1 4 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2012 VL 76 IS 6 BP 1290 EP 1297 DI 10.1002/jwmg.379 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 979XK UT WOS:000306856900020 ER PT J AU Pimentel, LA Maia, LA Campos, EM Dantas, AFM Medeiros, RMT Pfister, JA Cook, D Riet-Correa, F AF Pimentel, Luciano A. Maia, Lisanka A. Campos, Edipo M. Dantas, Antonio F. M. Medeiros, Rosane M. T. Pfister, James A. Cook, Daniel Riet-Correa, Franklin TI Conditioned food aversion to control outbreaks of poisoning by Ipomoea carnea subsp fistulosa and Turbina cordata in goats SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Poisonous plants; Ipomoea carnea subsp fistulosa; Turbina cordata; conditioned food aversion; plant poisoning control; goats; swainsonine ID MASCAGNIA-RIGIDA; TASTE-AVERSION; LOCOWEED; CATTLE; SHEEP; CONVOLVULACEAE; SERICOPHYLLA; SWAINSONINE; RIEDELII AB Pimentel L. A., Maia L. A., Campos E. M., Dantas A. F. M., Medeiros R. M. T., Pfister J.A., Cook D. & Riet-Correa F. 2012. [Conditioned food aversion to control outbreaks of poisoning by Ipomoea carnea subsp. fistulosa and Turbina cordata in goats.] Aversao alimentar condicionada no controle de surtos de intoxicacoes por Ipomoea carnea subsp. fistulosa e Turbina cordata. Pesquisa Veterinaria Brasileira 32(8): 707-714. Hospital Veterinario, Centro de Saude e Tecnologia Rural, Universidade Federal de Campina Grande, Patos, PB 58708-110, Brazil. E-mail: franklin.riet@pq.cnpq.br Conditioned food aversion is used to train livestock to avoid the ingestion of toxic plants. This technique was used to control Turbina cordata poisoning in goats in one farm, and to control Ipomoea carnea subsp. fistulosa poisoning in another farm. The goats were penned at night and the next morning the green plants were offered for 10 minutes. Goats that ingested any amount of the plant were treated through a gastric tube with 175mg of LiCl/kg body weight. In the flock in which the poisoning by T. cordata was occurring, the goats were averted every two months during the period that the plant was found in the pastures. During the experiment, from December 2009 to April 2011, new cases of poisoning were not observed, and there was a progressive decrease in the number of goats that ingested the plant and were averted. In the farm where I. carnea poisoning was occurring, most of the goats were averted in December 2010, 15-20 days before the first rains. The goats of this flock did not ingest the plant spontaneously in the field until September-October 2011, when, due to the dry season, there was a severe forage shortage, and the goats started to ingest the plant in the field. Later, despite three aversive treatments with 21 days intervals, the goats continued to ingest the plant and some animals became poisoned. In conclusion, conditioned food aversion was effective in to control intoxication by T. cordata. The technique was also effective in conditioning goats to avoid consuming I. carnea during the rainy season, but not during the dry season, with low forage availability in the field. The differences in these results seem to be due to the epidemiology of both poisonings: T. cordata is senescent and unavailable during most of the dry period, and green biomass is typically available either at the very end of the dry season, for a short period of time, and during the rainy season when there is no shortage of forage. In contrast, I. carnea grows in wet areas near water sources, and stays green during the dry period when there is a lack of other forage. C1 [Pimentel, Luciano A.; Maia, Lisanka A.; Campos, Edipo M.; Dantas, Antonio F. M.; Medeiros, Rosane M. T.; Riet-Correa, Franklin] Univ Fed Campina Grande, Hosp Vet, Ctr Saude & Technol Rural, BR-58708110 Patos de Minas, PB, Brazil. [Pfister, James A.; Cook, Daniel] Utah State Univ, ARS, USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Riet-Correa, F (reprint author), Univ Fed Campina Grande, Hosp Vet, Ctr Saude & Technol Rural, Campus Patos,Ave Univ S-N, BR-58708110 Patos de Minas, PB, Brazil. EM franklin.riet@pq.cnpq.br NR 28 TC 9 Z9 10 U1 0 U2 5 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X J9 PESQUISA VET BRASIL JI Pesqui. Vet. Bras. PD AUG PY 2012 VL 32 IS 8 BP 707 EP 714 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 008GC UT WOS:000308944400005 ER PT J AU Peled, N Ionescu, R Nol, P Barash, O McCollum, M VerCauteren, K Koslow, M Stahl, R Rhyan, J Haick, H AF Peled, Nir Ionescu, Radu Nol, Pauline Barash, Orna McCollum, Matt VerCauteren, Kurt Koslow, Matthew Stahl, Randal Rhyan, Jack Haick, Hossam TI Detection of volatile organic compounds in cattle naturally infected with Mycobacterium bovis SO SENSORS AND ACTUATORS B-CHEMICAL LA English DT Article DE Bovine tuberculosis; Mycobacterium bovis; Breath analysis; Volatile organic compound; Chemical sensors array; Gas-chromatography/mass-spectrometry ID POLYCYCLIC AROMATIC-HYDROCARBONS; EXHALED BREATH; MASS-SPECTROMETRY; CARBON NANOTUBES; TUBERCULOSIS; NANOPARTICLES; GOLD; INTERFERON; DIAGNOSIS; RESPONSES AB We report here on a novel methodology in detecting Mycobacterium bovis (M. bovis) infection in cattle, based on identifying unique volatile organic compounds (VOCs) or a VOC profile in the breath of cattle. The study was conducted on an M. bovis-infected dairy located in southern Colorado, USA, and on two tuberculosis-free dairies from northern Colorado examined as negative controls. Gas-chromatography/mass-spectrometry analysis revealed the presence of 2 VOCs associated with M. bovis infection and 2 other VOCs associated with the healthy state in the exhaled breath of M. bovis-infected and not infected animals, yielding distinctly different VOC patterns for the two study groups. Based on these results, a nanotechnology-based array of sensors was then tailored for detection of M. bovis-infected cattle via breath. Our system successfully identified all M. bovis-infected animals, while 21% of the not infected animals were classified as M. bovis-infected. This technique could form the basis for a real-time cattle monitoring system that allows efficient and non-invasive screening for new M. bovis infections on dairy farms. (C) 2012 Elsevier B.V. All rights reserved. C1 [Nol, Pauline; McCollum, Matt; VerCauteren, Kurt; Stahl, Randal; Rhyan, Jack] Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA. [Peled, Nir; Koslow, Matthew] Tel Aviv Univ, Chaim Sheba Med Ctr, Thorac Canc Res & Detect Ctr, IL-52621 Tel Aviv, Israel. [Ionescu, Radu; Barash, Orna; Haick, Hossam] Technion Israel Inst Technol, Dept Chem Engn, IL-32000 Haifa, Israel. [Ionescu, Radu; Barash, Orna; Haick, Hossam] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-32000 Haifa, Israel. RP Rhyan, J (reprint author), Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA. EM Jack.C.Rhyan@aphis.usda.gov; hhossam@technion.ac.il RI Ionescu, Radu/A-5158-2013 OI Ionescu, Radu/0000-0001-6211-0035 NR 45 TC 17 Z9 17 U1 1 U2 43 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 0925-4005 J9 SENSOR ACTUAT B-CHEM JI Sens. Actuator B-Chem. PD AUG-SEP PY 2012 VL 171 BP 588 EP 594 DI 10.1016/j.snb.2012.05.038 PG 7 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA 002XQ UT WOS:000308572700075 ER PT J AU Singh, P Sharratt, B Schillinger, WF AF Singh, Prabhakar Sharratt, Brenton Schillinger, William F. TI Wind erosion and PM10 emission affected by tillage systems in the world's driest rainfed wheat region SO SOIL & TILLAGE RESEARCH LA English DT Article DE Agricultural soils; Air quality; PM10; Tillage ID COLUMBIA PLATEAU; FUGITIVE DUST; AGRICULTURAL FIELDS; EASTERN WASHINGTON; PACIFIC-NORTHWEST; SUMMER FALLOW; WATER-CONTENT; SOIL; ROUGHNESS; SURFACE AB The Horse Heaven Hills of south-central Washington is the driest rainfed wheat growing region in the world. Low precipitation, high winds, poorly aggregated soils, sparse residue cover, and a tillage-based winter wheat (Triticum aestivum L.) - summer fallow (WW-SF) cropping system often combine to create soil surfaces which are susceptible to wind erosion. No-tillage summer fallow (NTF) and conservation tillage fallow (CTF) with an undercutter sweep implement were examined as alternative practices to traditional tillage fallow (TTF) with a tandem disk implement for reducing wind erosion and PM10 (particulate matter <= 10 mu m in aerodynamic diameter) emissions during the fallow phase of the WW-SF rotation. Wind erosion and PM10 emissions were assessed with a wind tunnel after primary spring tillage in mid-to-late April and after sowing winter wheat in August. Sediment loss and PM10 vertical flux and loss were generally less for NTF than with TIT, likely due to retention of surface residue and maintaining a soil crust in NTF. Sediment and PM10 loss increased after sowing wheat in both the TTF and CTF treatments. Although NTF abated the loss of sediment and PM0 compared with TTF, NTF is not yet an economical option for most growers in the region. Conservation tillage fallow using the undercutter sweep is an economically viable alternative to TTF for reducing windblown sediment and PM10 loss from agricultural soils in the Horse Heaven Hills. Published by Elsevier B.V. C1 [Sharratt, Brenton] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Singh, Prabhakar; Schillinger, William F.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Sharratt, B (reprint author), Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM Brenton.sharratt@ars.usda.gov OI Schillinger, William/0000-0001-9285-8159 FU USDA - Agricultural Research Service, Washington State University; USDA National Institute of Food and Agriculture through a Special Grant [PM10] FX Funding for this study was provided by the USDA - Agricultural Research Service, Washington State University, and by USDA National Institute of Food and Agriculture through a Special Grant to the Columbia Plateau PM10 Project. The authors gratefully acknowledge wheat growers Mike Nichols and David Pearson for their collaboration. NR 34 TC 18 Z9 18 U1 2 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 J9 SOIL TILL RES JI Soil Tillage Res. PD AUG PY 2012 VL 124 BP 219 EP 225 DI 10.1016/j.still.2012.06.009 PG 7 WC Soil Science SC Agriculture GA 009HC UT WOS:000309015800028 ER PT J AU Biggins, DE Hanebury, LR Fagerstone, KA AF Biggins, Dean E. Hanebury, Louis R. Fagerstone, Kathleen A. TI DIGGING BEHAVIORS OF RADIO-TAGGED BLACK-FOOTED FERRETS NEAR MEETEETSE, WYOMING, 1981-1984 SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article AB Intensive radio-tracking during August December enabled us to collect detailed information on digging behaviors of a small sample of black-footed ferrets (Mustela nigripes) occupying colonies of white-tailed prairie dogs (Cynomys leucurus). A sample of 33 prairie dogs, also radio-tagged, progressively ceased aboveground activity during late summer and fall, presumably as they descended into burrows to hibernate. Most of the time ferrets spent digging was in November December when >95% of the radio-tagged prairie dogs were inactive, suggesting that digging was primarily to excavate hibernating prey Although 43.9% of the burrow openings were estimated to be in large mounds, which are common on colonies of white-tailed prairie dogs, all of a sample of 17 deposits of soil (diggings) made by ferrets were excavated at small mounds or nonmounded openings. The average duration of 23 nocturnal sessions of digging by ferrets was 112.2 minutes. A digging session consisted of multiple bouts of soil movement typically lasting about 5 min, and sessions were separated by pauses above- or belowground lasting several minutes. Bouts of moving soil from a burrow involved round-trips of 12.5-30.3 s to remove an average of 35 cm(3) of soil per trip. These digging bouts are energetically costly for ferrets. One female moved 16.8 kg of soil an estimated 3.3 m during bouts having a cumulative duration of 178 minutes, removing a soil plug estimated to be 178 cm long. Increasing evidence suggests that some behaviors of ferrets and prairie dogs are coevolutionary responses between this highly specialized predator and its prairie dog prey. C1 [Biggins, Dean E.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Hanebury, Louis R.] Western Area Power Adm, Billings, MT 59101 USA. [Fagerstone, Kathleen A.] USDA, APHIS, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80525 USA. RP Biggins, DE (reprint author), US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. EM dean_biggins@usgs.gov NR 23 TC 6 Z9 6 U1 1 U2 12 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD AUG PY 2012 VL 72 IS 2 BP 148 EP 157 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 999BH UT WOS:000308283600004 ER PT J AU Pendleton, RL McArthur, ED Sanderson, SC AF Pendleton, Rosemary L. McArthur, E. Durant Sanderson, Stewart C. TI BREEDING SYSTEM AND INTERACCESSIONAL HYBRIDIZATION OF PURSHIA TRIDENTATA PLANTS GROWN IN A COMMON GARDEN SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID CRYPTIC SELF-INCOMPATIBILITY; NITROGEN-FIXATION; WIND POLLINATION; ROSACEAE; ANGIOSPERMS AB Purshia spp. (Rosaceae) comprise a widespread western North American species complex that is important as landscape dominants, wildlife habitat, browse for wild and domestic ungulates, and seed reserves for small mammals. This study examined aspects of the phenology, compatibility, pollination biology, and progeny fruit characteristics of multiple accessions of bitterbrush (Purshia tridentate), as well as a putative hybrid between bitterbrush and Purshia stansburiana. Except for open- and wind-pollination treatments, mass pollination was accomplished at anthesis within white paper sacks, which were used to isolate treatment branches. Bitterbrush appears to be largely self-incompatible and requires some agent, either wind or animal, to effect pollination. While the majority of pollination of bitterbrush flowers is undoubtedly accomplished by insects, some pollination by wind is possible (ambophily) when shrubs are closely spaced, as flowering is fairly synchronous. Flower duration varied among shrubs, but generally lasted from 12 to 24 hours, depending on weather conditions. Flower development stage had little effect on fruit set, indicating prolonged stigma receptivity. Populations of bitterbrush exhibit considerable interfertility, as well as substantial hybridization with congeners. Intrapopulation crosses were only slightly more successful than interpopulation crosses. One of the populations analyzed exhibits characteristics consistent with its putative hybrid nature, having more than one pistil per receptacle, smaller but longer fruits, and a greater reliance on insects for pollination. Both maternal and paternal effects are important in fruit development. Taxonomic relationships within the complex are discussed. C1 [Pendleton, Rosemary L.] US Forest Serv, USDA, Rocky Mt Res Stn, Albuquerque, NM USA. [McArthur, E. Durant; Sanderson, Stewart C.] US Forest Serv, USDA, Rocky Mt Res Stn, Provo, UT USA. RP Pendleton, RL (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Albuquerque, NM USA. EM rpendleton@fs.fed.us NR 38 TC 0 Z9 0 U1 1 U2 7 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD AUG PY 2012 VL 72 IS 2 BP 241 EP 249 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 999BH UT WOS:000308283600013 ER PT J AU Lehman, CP Schmitz, LE Rumble, MA Kragel, JJ Millspaugh, JJ AF Lehman, Chad P. Schmitz, Lowell E. Rumble, Mark A. Kragel, Jackie J. Millspaugh, Joshua J. TI OBSERVATION OF DYSTOCIA IN WILD ELK SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID POPULATION AB On the basis of reports in the literature, incidence of dystocia in wild elk (Germs elaphus) across the west is rare. In 2011, one of 34 (3%) pregnant cow elk in our study experienced dystocia during birth. Our visual observations indicated that it took approximately 4 days for a radio-collared cow elk to succumb to dystocia in our study. Little is known about dystocia in wild elk populations, and our observation provides some insight about fetal malpresentations. C1 [Lehman, Chad P.; Kragel, Jackie J.] S Dakota Dept Game Fish & Pk, Custer, SD 57730 USA. [Schmitz, Lowell E.] S Dakota Dept Game Fish & Pk, Rapid City, SD 57702 USA. [Rumble, Mark A.] US Forest Serv, USDA, Rocky Mt Res Stn, Rapid City, SD 57702 USA. [Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. RP Lehman, CP (reprint author), S Dakota Dept Game Fish & Pk, Custer State Pk,13329 US Hwy 16A, Custer, SD 57730 USA. EM chad.lehman@state.sd.us FU South Dakota Department of Game, Fish and Parks, Federal Aid to Wildlife Restoration Fund [W-75-R-53, 7544]; Rocky Mountain Elk Foundation FX We thank S. Lindsay, DVM, for constructive comments and additions to this manuscript. Funding for this research was from the South Dakota Department of Game, Fish and Parks, Federal Aid to Wildlife Restoration Fund (Project W-75-R-53, No. 7544), and the Rocky Mountain Elk Foundation. Additional field assistance and technical support was provided by the USDA Forest Service, Rocky Mountain Research Station. Technical support was provided by the University of Missouri Columbia. All handling, marking, and monitoring procedures were approved by the South Dakota State University Research Committee (Institutional Animal Care and Use Committee Approval Number 11-012A). NR 12 TC 0 Z9 0 U1 1 U2 4 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD AUG PY 2012 VL 72 IS 2 BP 250 EP 251 PG 2 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 999BH UT WOS:000308283600014 ER PT J AU Adams, CK Saenz, D AF Adams, C. K. Saenz, D. TI Leaf litter of invasive Chinese tallow (Triadica sebifera) negatively affects hatching success of an aquatic breeding anuran, the Southern Leopard Frog (Lithobates sphenocephalus) SO CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE LA English DT Article DE Southern Leopard Frog; Lithobates sphenocephalus; Chinese tallow; Triadica sebifera; invasive species; dissolved oxygen; pH; egg hatching ID RANA-SPHENOCEPHALA; AMPHIBIAN LARVAE; NEST PREDATION; NATIVE FROGS; TREE; OXYGEN; EGGS; RESPIRATION; CALIFORNIA; IMPACT AB Chinese tallow (Triadica sebifera (L.) Small) is an aggressive invasive tree species that can be abundant in parts of its non-native range. This tree species has the capability of producing monocultures, by outcompeting native trees, which can be in or near wetlands that are utilized by breeding amphibians. Existing research suggests that leaf litter from invasive Chinese tallow reduces survival in larval anurans. The purpose of this study was to determine the effects of Chinese tallow leaf litter on anuran eggs. We exposed eggs of the Southern Leopard Frog (Lithobates sphenocephalus (Cope, 1886)) at various stages of development to different concentrations of Chinese tallow leaf litter to determine survival. Eggs in the earliest stages of development that we exposed to tallow leaf litter died, regardless of concentration; however, some more-developed eggs exposed to tallow leaf litter did hatch. We determined that the greater the concentration of tallow leaf litter, the lower the dissolved oxygen and pH levels we observed. We suggest that changes in these water-quality parameters are the cause of the observed mortality of anuran eggs in our experiments. Eggs exposed to water containing tallow leaf litter with dissolved oxygen <1.59 mg/L and a pH <5.29 did not survive to hatching. C1 [Adams, C. K.; Saenz, D.] USDA Forest Serv, So Res Stn, Nacogdoches, TX 75965 USA. RP Adams, CK (reprint author), USDA Forest Serv, So Res Stn, 506 Hayter St, Nacogdoches, TX 75965 USA. EM coryadams@fs.fed.us NR 53 TC 5 Z9 5 U1 1 U2 29 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0008-4301 J9 CAN J ZOOL JI Can. J. Zool.-Rev. Can. Zool. PD AUG PY 2012 VL 90 IS 8 BP 991 EP 998 DI 10.1139/Z2012-067 PG 8 WC Zoology SC Zoology GA 005VU UT WOS:000308779000008 ER PT J AU Luo, PJ Jiang, WX Beier, RC Shen, JZ Jiang, HY Miao, H Zhao, YF Chen, X Wu, YN AF Luo, Peng Jie Jiang, Wen Xiao Beier, Ross C. Shen, Jian Zhong Jiang, Hai Yang Miao, Hong Zhao, Yun Feng Chen, Xia Wu, Yong Ning TI Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone Etabolite, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ) in Animal Tissues SO BIOMEDICAL AND ENVIRONMENTAL SCIENCES LA English DT Article DE AMOZ; Animal tissue; ELISA; Fish tissue; Furaltadone ID PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; METABOLITE; NITROFURAN; FURAZOLIDONE; ANTIBODY; ANTIBIOTICS; IMMUNOASSAY AB Objective To determine 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues released from protein bound AMOZ in animal tissues. Methods Polyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed. Results Rabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC50 of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 mu g/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues. Conclusion The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues. C1 [Chen, Xia] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China. [Luo, Peng Jie; Miao, Hong; Zhao, Yun Feng; Wu, Yong Ning] China Natl Ctr fbr Food Safety Risk Assessment, Minist Hlth Key Lab, Beijing 100021, Peoples R China. [Jiang, Wen Xiao; Shen, Jian Zhong; Jiang, Hai Yang] China Agr Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, Beijing 100193, Peoples R China. [Beier, Ross C.] USDA ARS, So Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Chen, X (reprint author), Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China. EM chenxia602@163.com; china_cdc@yahoo.cn FU National Science Foundation for Young Scientists of China [21107104]; State Key Program of National Natural Science of China [20837003]; Ministry of Health [200902009]; National Science & Technology Pillar Program [2009BADB9B03-Z02] FX This work was supported by the National Science Foundation for Young Scientists of China (No. 21107104), the State Key Program of National Natural Science of China (No. 20837003), grants from the Ministry of Health (No. 200902009), and the National Science & Technology Pillar Program (No. 2009BADB9B03-Z02). NR 19 TC 3 Z9 3 U1 1 U2 15 PU CHINESE CENTER DISEASE CONTROL & PREVENTION PI BEIJING PA 155 CHANGBAI RD, CHANGPING DISTRICT, BEIJING, 102206, PEOPLES R CHINA SN 0895-3988 J9 BIOMED ENVIRON SCI JI Biomed. Environ. Sci. PD AUG PY 2012 VL 25 IS 4 BP 449 EP 457 DI 10.3967/0895-3988.2012.04.010 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA 005YM UT WOS:000308786000010 PM 23026525 ER PT J AU Han, X Zhao, M Lu, L Liu, YH AF Han, Xu Zhao, Min Lu, Lei Liu, Yanhong TI Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190 SO FUNGAL BIOLOGY LA English DT Article DE Bilirubin oxidase; Dye decolorization; Myrothecium verrucaria; Purification and characterization ID AZO DYES; PHANEROCHAETE-CHRYSOSPORIUM; TRAMETES-VERSICOLOR; LACCASE ACTIVITY; WASTE MATERIALS; HAZARDOUS DYE; BIODEGRADATION; DEGRADATION; ADSORPTION; EXPRESSION AB Myrothecium verrucaria 3.2190 is a nonligninolytic fungus that produces bilirubin oxidase. Both M. verrucaria and the extracellular bilirubin oxidase were tested for their ability to decolorize indigo carmine. The biosorption and biodegradation of the dye were detected during the process of decolorization; more than 98% decolorization efficiency was achieved after 7 days at 26 degrees C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30 degrees C similar to 50 degrees C, pH 5.5 similar to 9.5 with dye concentrations of 50 mg l(-1)similar to 200 mg l(-1) Bilirubin oxidase was purified and visualized as a single band on native polyacrylamide gel electrophoresis (PAGE). Several enzymatic properties of the purified enzyme were investigated. Moreover, the identity of the purified bilirubin oxidase (BOD) was confirmed by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). These results demonstrate that the purified bilirubin oxidase in M. verrucaria strain has potential application in dye effluent decolorization. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Han, Xu; Zhao, Min; Lu, Lei] NE Forestry Univ, Coll Life Sci, Harbin 150040, Heilongjiang, Peoples R China. [Han, Xu] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China. [Liu, Yanhong] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Zhao, M (reprint author), NE Forestry Univ, Coll Life Sci, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China. EM 82191513@163.com FU National Natural Science Foundation of China [30671702] FX The authors are grateful to the National Natural Science Foundation of China (No. 30671702) for financial support. We also thank Dr Pina Fratamico and Dr James Smith for critical reading of the manuscript. NR 45 TC 0 Z9 1 U1 4 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD AUG PY 2012 VL 116 IS 8 BP 863 EP 871 DI 10.1016/j.funbio.2012.05.003 PG 9 WC Mycology SC Mycology GA 005EA UT WOS:000308732100002 PM 22862914 ER PT J AU Scanlon, TM Kustas, WP AF Scanlon, Todd M. Kustas, William P. TI Partitioning Evapotranspiration Using an Eddy Covariance-Based Technique: Improved Assessment of Soil Moisture and Land-Atmosphere Exchange Dynamics SO VADOSE ZONE JOURNAL LA English DT Article ID WATER FLUX; EVAPORATION; CANOPY; TRANSPIRATION; FOREST; COMPONENTS; MODEL; CORN; CONDUCTANCE; ECOSYSTEM AB The capability to partition evapotranspiration (ET) measurements into two components, namely direct evaporation (E) from soil, leaf, and litter surfaces and transpiration (T) via plants is critical toward better defining hydrological processes along the soil-plant-atmosphere continuum. Such information has practical applications ranging from water resource management to climate modeling. A recently developed partitioning technique was employed using high frequency measurements of water vapor and carbon dioxide collected by an eddy covariance flux system, which was deployed over a corn (Zea mays L.) field near Beltsville, MD for a full growing season. The partitioning technique is based on flux-variance similarity theory and has the benefit of relying on routinely collected eddy covariance measurements, with no additional instrumentation required. Results showed an increase in the T/ET ratio from similar to 5% during the early portion of the growing season to similar to 70-80% by the time the corn crop reached maturity. This was consistent with observed dynamics of the soil moisture profile, which indicated that water was removed from deeper soil layers as the growing season progressed. The partitioned estimates of T are shown to be useful for the appropriate calculation of canopy conductance, a key variable in land surface models. Finally, the transient impact of rainfall events were examined, in which the suppression of T and enhancement of E were observed for up to several days following rainfall. Insight gained through the partitioning of ET fluxes has the potential to significantly improve the structure and parameterization of land surface hydrological models. C1 [Scanlon, Todd M.] Univ Virginia, Dep Environm Sci, Charlottesville, VA 22904 USA. [Kustas, William P.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Scanlon, TM (reprint author), Univ Virginia, Dep Environm Sci, Charlottesville, VA 22904 USA. EM tms2v@virginia.edu RI Kustas, William/C-2063-2015 FU National Science Foundation [EAR-0645697] FX This research was supported by the National Science Foundation Hydrologic Science and Education and Human Resources programs (EAR-0645697). Dr. Timothy Gish and Dr. John Prueger were key collaborators at the OPE3 site, and Andrew Russ was responsible for maintaining the meteorological instruments and assisted in the quality control and initial processing of the data. NR 37 TC 10 Z9 10 U1 2 U2 44 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2012 VL 11 IS 3 DI 10.2136/vzj2012.0025 PG 7 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 002JB UT WOS:000308526800034 ER PT J AU Jackson, LS Voss, KA Ryu, D AF Jackson, L. S. Voss, K. A. Ryu, D. TI Effects of different extrusion conditions on the chemical and toxicological fate of fumonisin B-1 in maize: a short review SO WORLD MYCOTOXIN JOURNAL LA English DT Review DE reduction; toxicity; N-(deoxy-D-fructos-1-yl)-fumonisin B-1 ID SINGLE-SCREW EXTRUSION; NEURAL-TUBE DEFECTS; IN-VIVO TOXICITY; CORN-BASED FOODS; FUSARIUM-MONILIFORME; MASS-SPECTROMETRY; ESOPHAGEAL CANCER; GLUCOSE REACTION; REACTION-PRODUCT; TORTILLA CHIPS AB Fumonisin B-1 (FB1) is a common mycotoxin found in maize and maize-based food products. Although FB1 is relatively heat stable and survives most thermal processes, extrusion cooking has been shown to be effective at reducing levels of the toxin in contaminated maize. This review summarises studies on the chemical and toxicological fate of FB1 in maize extruded under different conditions. Overall, these studies indicate that stability of FB1 depends on the extrusion conditions, e. g. temperature and screw speed, and that the presence of reducing sugars augment apparent loss of the toxin. The chemical fate of FB1 was investigated by measuring FB1, hydrolysed FB1, and N-substituted FB1 compounds with a mass balance approach while the relative toxicity was determined by rat feeding trials. FB1 in contaminated grits was reduced by 21-37% and 77-87% in the absence and presence of 10% (w/w) glucose, respectively, during single-screw extrusion. Greater reductions of 64-72% and 89-94% were achieved by twin-screw extrusion. Mass balance analysis showed that most of the FB1 in grits extruded without glucose was recovered as FB1, whereas the FB1-glucose reaction product, N-(deoxy-D-fructos-1-yl)-fumonisin B-1 was the prevalent form after extrusion with glucose. 23 to 38% of FB1 in extruded grits with and without added glucose was bound to component(s) of the maize grits. Only 37-46% of FB1 present in unextruded grits could be recovered as one of the fumonisin species (hydrolysed FB1, N-substituted FB1 compounds) or as matrix-bound forms in extruded grits. Reduced FB1 concentrations in extruded grits and the even lower concentrations in grits extruded with glucose resulted in a dose-dependent reduction of toxicity as shown by the less severe apoptotic lesions and sphingolipid effects that were found in the kidneys of rats. In summary, extrusion processing, especially with glucose supplementation, is potentially useful to reduce FB1 concentrations and toxicity of contaminated maize. C1 [Jackson, L. S.] US FDA, Natl Ctr Food Safety & Technol, Bedford Pk, IL 60501 USA. [Voss, K. A.] USDA ARS, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [Ryu, D.] Texas Womans Univ, Dept Nutr & Food Sci, Denton, TX 76204 USA. RP Jackson, LS (reprint author), US FDA, Natl Ctr Food Safety & Technol, 6502 S Archer Rd, Bedford Pk, IL 60501 USA. EM dryu@twu.edu NR 52 TC 7 Z9 9 U1 3 U2 14 PU WAGENINGEN ACADEMIC PUBLISHERS PI WAGENINGEN PA PO BOX 220, WAGENINGEN, 6700 AE, NETHERLANDS SN 1875-0710 J9 WORLD MYCOTOXIN J JI World Mycotoxin J. PD AUG PY 2012 VL 5 IS 3 BP 251 EP 260 DI 10.3920/WMJ2012.1431 PG 10 WC Food Science & Technology; Mycology; Toxicology SC Food Science & Technology; Mycology; Toxicology GA 995SB UT WOS:000308031500006 ER PT J AU Muttis, E Miele, SAB Belaich, MN Micieli, MV Becnel, JJ Ghiringhelli, PD Garcia, JJ AF Muttis, Evangelina Miele, Solange A. B. Belaich, Mariano N. Micieli, Maria V. Becnel, James J. Ghiringhelli, Pablo D. Garcia, Juan J. TI First record of a mosquito iridescent virus in Culex pipiens L. (Diptera: Culicidae) SO ARCHIVES OF VIROLOGY LA English DT Article DE Iridescent virus; Culicidae; Culex pipiens; Argentina AB The mosquito iridescent viruses (MIVs) are large icosahedral DNA viruses that replicate and assemble in the cytoplasm of the host. Paracrystalline arrangements of virions that accumulate in the cytoplasm produce an iridescent color that is symptomatic of acute infections. In August 2010, we found larvae of Culex pipiens with these symptoms in suburban ditches around the city of La Plata, Argentina. Electron microscope studies, DNA sequencing, and phylogenetic analysis of the major capsid protein confirmed this as the first record of an MIV in C. Pipiens. C1 [Muttis, Evangelina; Micieli, Maria V.; Garcia, Juan J.] Ctr Estudios Parasitol & Vectores CEPAVE CONICET, RA-1900 La Plata, Buenos Aires, Argentina. [Miele, Solange A. B.; Belaich, Mariano N.; Ghiringhelli, Pablo D.] UNQ, Dept Ciencia & Tecnol, LIGBCM AVI, RA-1876 Buenos Aires, DF, Argentina. [Becnel, James J.] ARS, USDA, Ctr Med Agr & Vet Entomol CEMAVE, Gainesville, FL 32604 USA. [Garcia, Juan J.] Res Comis Invest Cient Prov Buenos Aires CIC, Buenos Aires, DF, Argentina. RP Muttis, E (reprint author), Ctr Estudios Parasitol & Vectores CEPAVE CONICET, Calle 2 584, RA-1900 La Plata, Buenos Aires, Argentina. EM evangelinamuttis@hotmail.com FU Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [PICT 2008-0653]; CIC; CONICET FX This work was supported in part by grant PICT 2008-0653 from the Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT). PDG, MNB and SABM are teachers and researchers of the Universidad Nacional de Quilmes. MNB, PDG and MVM are members of the Research Career of CONICET (Consejo Nacional de Ciencia y Tecnologia). JJG is a member of the Research Career of CIC (Comision de Investigaciones Cientificas), EM holds a fellowship from CIC. SABM holds a fellowship from CONICET. We are grateful to Neil Sanscrainte and William Reid for designing the primers and for assistance with the PCR used in this work. NR 10 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD AUG PY 2012 VL 157 IS 8 BP 1569 EP 1571 DI 10.1007/s00705-012-1302-2 PG 3 WC Virology SC Virology GA 985HK UT WOS:000307253800016 PM 22543633 ER PT J AU Yackulic, CB Reid, J Davis, R Hines, JE Nichols, JD Forsman, E AF Yackulic, Charles B. Reid, Janice Davis, Raymond Hines, James E. Nichols, James D. Forsman, Eric TI Neighborhood and habitat effects on vital rates: expansion of the Barred Owl in the Oregon Coast Ranges SO ECOLOGY LA English DT Article DE autologistic; Barred Owl; detection; habitat; metapopulation; Northern Spotted Owl; presence ID NORTHERN SPOTTED OWLS; STRIX-VARIA; SITE CHARACTERISTICS; IMPERFECT DETECTION; CASCADE MOUNTAINS; LOCAL EXTINCTION; OCCUPANCY; MODELS; DYNAMICS; METAPOPULATION AB In this paper, we modify dynamic occupancy models developed for detection-nondetection data to allow for the dependence of local vital rates on neighborhood occupancy, where neighborhood is defined very flexibly. Such dependence of occupancy dynamics on the status of a relevant neighborhood is pervasive, yet frequently ignored. Our framework permits joint inference about the importance of neighborhood effects and habitat covariates in determining colonization and extinction rates. Our specific motivation is the recent expansion of the Barred Owl (Strix varia) in western Oregon, USA, over the period 1990-2010. Because the focal period was one of dramatic range expansion and local population increase, the use of models that incorporate regional occupancy (sources of colonists) as determinants of dynamic rate parameters is especially appropriate. We began our analysis of 21 years of Barred Owl presence/nondetection data in the Tyee Density Study Area (TDSA) by testing a suite of six models that varied only in the covariates included in the modeling of detection probability. We then tested whether models that used regional occupancy as a covariate for colonization and extinction outperformed models with constant or year-specific colonization or extinction rates. Finally we tested whether habitat covariates improved the AIC of our models, focusing on which habitat covariates performed best, and whether the signs of habitat effects are consistent with a priori hypotheses. We conclude that all covariates used to model detection probability lead to improved AIC, that regional occupancy influences colonization and extinction rates, and that habitat plays an important role in determining extinction and colonization rates. As occupancy increases from low levels toward equilibrium, colonization increases and extinction decreases, presumably because there are more and more dispersing juveniles. While both rates are affected, colonization increases more than extinction decreases. Colonization is higher and extinction is lower in survey polygons with more riparian forest. The effects of riparian forest on extinction rates are greater than on colonization rates. Model results have implications for management of the invading Barred Owl, both through habitat alteration and removal. C1 [Yackulic, Charles B.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Yackulic, Charles B.; Hines, James E.; Nichols, James D.] USGS, Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. [Reid, Janice; Davis, Raymond; Forsman, Eric] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Yackulic, CB (reprint author), US Geol Survey, Grand Canyon Monitoring & Res Ctr, SW Biol Sci Ctr, Flagstaff, AZ 86001 USA. EM c_yackulic@yahoo.com FU National Science Foundation [DBI-1003221] FX This material is based upon work supported by the National Science Foundation under Award No. DBI-1003221. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. We thank everyone who contributed to collecting the information especially Scott Graham, Rob Horn, Richard Leach, Jason Mowdy, Amy Price, Paula Shaklee, and Bob Straub. We thank Darryl MacKenzie for useful discussions about implementation of autologistic models. We thank Mitch Eaton, Evan Grant, and three anonymous referees for comments on the initial manuscript. NR 56 TC 25 Z9 25 U1 3 U2 67 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD AUG PY 2012 VL 93 IS 8 BP 1953 EP 1966 PG 14 WC Ecology SC Environmental Sciences & Ecology GA 984TF UT WOS:000307214000020 PM 22928423 ER PT J AU Knothe, G AF Knothe, Gerhard TI Fuel Properties of Highly Polyunsaturated Fatty Acid Methyl Esters. Prediction of Fuel Properties of Algal Biodiesel SO ENERGY & FUELS LA English DT Article ID MICROALGAL BIOFUELS; KINEMATIC VISCOSITY; PHYSICAL-PROPERTIES; FEEDSTOCKS; OIL; PETRODIESEL; TECHNOLOGY; CHALLENGES; COMPONENTS; STABILITY AB Biodiesel, defined as the monoalkyl esters of vegetable oils and animal fats, can be derived from other triacylglycerol-containing feedstocks. Algae are being considered for this purpose due to their claimed high production potential. However, there are no comprehensive reports regarding the fuel properties of biodiesel obtained from algal oils. Algal oils, with examples of some exceptions also mentioned here, often contain significant amounts of saturated and highly polyunsaturated (>= 4 double bonds) fatty acid chains which influence fuel properties of the resulting biodiesel. In this connection, the relevant fuel properties of biodiesel from algal oils and the important fuel properties of highly polyunsaturated fatty acid methyl esters as they would occur in many biodiesel fuels obtained from algal oils, have not yet been reported. To fill this gap, in the present work for the first time two neat highly polyunsaturated fatty acid methyl esters with more than three double bonds, methyl 5(Z),8(Z),11(414(Z)-eicosatetraenoate (C20:4) and methyl 4(Z),7(Z),10(Z),13(Z),l 6(Z),19(Z)-docosahexaenoate (C22:6), were investigated. The cetane numbers were determined as 29.6 for C20:4 and 24.4 for C22:6. Kinematic viscosity values were observed as 3.11 mm(2)/s for C20:4 and 2.97 mm(2)/s for C22:6, while oxidative stability values were below 0.1 h for both by the Rancimat test while densities were above 0.9 g/cm(3). Two polyunsaturated C20 methyl esters, methyl 11(Z),14(Z)-eicosadienoate and 11(Z),14(Z),17(Z)-eicosatrienoate, were also studied for kinematic viscosity, density, and oxidative stability to expand the database on these properties of C20 compounds and provide additional data to predict the properties of other highly polyunsaturated fatty acid methyl esters. Properties of biodiesel from algal oils are predicted with cetane numbers expected in the low to upper 40s and kinematic viscosity between 3 and 4 mm(2)/s for most algal biodiesel. C1 [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Knothe, G (reprint author), USDA ARS NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov NR 61 TC 39 Z9 39 U1 0 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD AUG PY 2012 VL 26 IS 8 BP 5265 EP 5273 DI 10.1021/ef300700v PG 9 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 988ME UT WOS:000307494300071 ER PT J AU Wasonga, CJ Pastor-Corrales, MA Porch, TG Griffiths, PD AF Wasonga, Charles J. Pastor-Corrales, Marcial A. Porch, Timothy G. Griffiths, Philip D. TI Multi-environment Selection of Small Sieve Snap Beans Reduces Production Constraints in East Africa and Subtropical Regions SO HORTSCIENCE LA English DT Article DE breeding; common bean rust; high temperature stress; Phaseolus vulgaris ID PHASEOLUS-VULGARIS; RUST RESISTANCE; HEAT TOLERANCE; CLIMATE-CHANGE AB Small-sieve snap beans (Phaseolus vulgaris L.) are an important source of income for smallholder farmers in East Africa. In this region as well as in other tropical and subtropical environments, common bean rust, caused by Uromyces appendiculatus (Pers.:Pers.), and heat stress reduce the yield and quality of snap beans. Small-sieve rust-resistant snap beans and that are heat-tolerant were developed using heat-tolerant snap bean breeding lines that had broad-spectrum rust resistance conditioned by the combination of the Andean Ur-4 and Mesoamerican Ur-11 genes. The Ur-4 and Ur-11 rust gene combination confers resistance to 90 races of the hypervariable pathogen from different parts of the world, including East Africa, that are maintained at Beltsville, MD. Four breeding lines each having the combination of the two rust genes were crossed in a 4 x 5 diallel mating design with five susceptible small-sieve cultivars to give 20 F-1 hybrids. The hybrid combinations were advanced through the F-2, F-3, and F-4 generations with selection for heat tolerance, rust resistance, and pod quality to develop lines combining these traits. Twenty F-5 breeding lines that had the combination of these traits were selected and evaluated in East Africa at four field sites selected on the basis of differences in altitude, climate, and virulence diversity of the bean rust pathogen and in Puerto Rico at a field site characterized by high temperatures. There was a significant positive correlation between ranks of heat stress influenced yield components (seeds per pod and total yield) at the hot field site and the controlled high-temperature (32/27 degrees C) greenhouse. Four of the breeding lines developed, L5, 19, L13, and 117, combined heat tolerance and rust resistance in the desired plant type with high yield and good pod quality. These lines are the first known small-sieve snap beans with the combination of traits for heat tolerance and broad-spectrum rust resistance conferred by the Ur-4 and Ur-11 genes. These results demonstrate ability to combine heat tolerance and rust resistance as important traits for adaptation of specific market classes of common bean to tropical and subtropical environments through targeted selection of multiple traits in controlled environments. C1 [Wasonga, Charles J.; Griffiths, Philip D.] Cornell Univ, Dept Hort Sci, Geneva, NY 14456 USA. [Pastor-Corrales, Marcial A.] USDA, ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Porch, Timothy G.] USDA, ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. RP Griffiths, PD (reprint author), Cornell Univ, Dept Hort Sci, NYSAES,315 Hedrick Hall, Geneva, NY 14456 USA. EM pdg8@cornell.edu NR 26 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2012 VL 47 IS 8 BP 1000 EP 1006 PG 7 WC Horticulture SC Agriculture GA 991EQ UT WOS:000307684700005 ER PT J AU Snider, JL Russo, VM Roberts, W Wann, EV Raper, RL AF Snider, John L. Russo, Vincent M. Roberts, Warren Wann, Elbert V. Raper, Randy L. TI Cultural and Environmental Factors Governing Tomato Production: Local Production under Elevated Temperatures SO HORTSCIENCE LA English DT Article DE Solanum lycopersieum; tomato; local foods; climate change; heat units; marketable yield ID HEAT UNITS; CLIMATE-CHANGE; FOOD SECURITY; AGRICULTURE; SYSTEMS; STRESS; FRUIT AB Long-term fresh tomato (Solanum lycopersicum L.) production data were used to estimate cultural and environmental impacts on marketable tomato yields in eastern Oklahoma. Quantifying the interactive effects of planting date and growing season duration and the effects of cumulative heat units and heat unit accumulation rate on marketable yields allowed for productivity estimates based on past temperature conditions. Simulated increases in air temperature were predicted to reduce yields and increase the amount of cropland needed to meet local consumption demands. Consequently, local tomato production in Oklahoma may be negatively impacted under elevated temperature conditions projected under global climate change. C1 [Snider, John L.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31794 USA. [Russo, Vincent M.; Wann, Elbert V.] ARS, USDA, Wes Watkins Agr Res Lab, Lane, OK 74555 USA. [Roberts, Warren] Oklahoma State Univ, Wes Watkins Agr Res & Extens Ctr, Lane, OK 74555 USA. [Raper, Randy L.] ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. RP Snider, JL (reprint author), Univ Georgia, Dept Crop & Soil Sci, 115 Coastal Way, Tifton, GA 31794 USA. EM jlsnider@uga.edu NR 35 TC 0 Z9 0 U1 1 U2 15 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2012 VL 47 IS 8 BP 1022 EP 1028 PG 7 WC Horticulture SC Agriculture GA 991EQ UT WOS:000307684700008 ER PT J AU Marble, SC Prior, SA Runion, GB Torbert, HA Gilliam, CH Fain, GB Sibley, JL Knight, PR AF Marble, S. Christopher Prior, Stephen A. Runion, G. Brett Torbert, H. Allen Gilliam, Charles H. Fain, Glenn B. Sibley, Jeff L. Knight, Patricia R. TI Effects of Fertilizer Placement on Trace Gas Emissions from Nursery Container Production SO HORTSCIENCE LA English DT Article DE carbon dioxide; climate change; greenhouse gases; horticulture; methane; nitrous oxide ID NITROUS-OXIDE EMISSIONS; CARBON-DIOXIDE; INTENSIVE AGRICULTURE; CO2 EMISSIONS; LOAM SOIL; MITIGATION; MANAGEMENT; N2O; NO; ATMOSPHERE AB Increased trace gas emissions of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) are widely believed to be a primary cause of global warming. Agriculture is a large contributor to these emissions; however, its role in climate change is unique in that it can act as a source of trace gas emissions or it can act as a major sink. Furthermore, agriculture can significantly reduce emissions through changes in production management practices. Much of the research on agriculture's role in mitigation of greenhouse gas (GHG) emissions has been conducted in row crops and pastures as well as forestry and animal production systems with little focus on contributions from specialty crop industries such as horticulture. Our objective was to determine efflux patterns of CO2, CH4, and N2O associated with three different fertilization methods (dibble, incorporated, and topdressed) commonly used in nursery container production. Weekly measurements indicated that CO2 fluxes were slightly lower when fertilizer was dibbled compared with the other two methods. Nitrous oxide fluxes were consistently highest when fertilizer was incorporated. Methane flux was generally low with few differences among treatments. Results from this study begin to provide data that can be used to implement mitigation strategies in container plant production, which will help growers adapt to possible emission regulations and benefit from future GHG mitigation or offset programs. C1 [Prior, Stephen A.; Runion, G. Brett; Torbert, H. Allen] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Marble, S. Christopher; Gilliam, Charles H.; Fain, Glenn B.; Sibley, Jeff L.] Auburn Univ, Dept Hort, Auburn, AL 36849 USA. [Knight, Patricia R.] Mississippi State Coastal Res & Extens Ctr, Biloxi, MS 39532 USA. RP Torbert, HA (reprint author), ARS, USDA, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM allen.torbert@ars.usda.gov FU USDA-ARS Floriculture and Nursery Research Initiative; Horticulture Research Institute FX This work was supported by the USDA-ARS Floriculture and Nursery Research Initiative and the Horticulture Research Institute. NR 61 TC 1 Z9 1 U1 1 U2 14 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2012 VL 47 IS 8 BP 1056 EP 1062 PG 7 WC Horticulture SC Agriculture GA 991EQ UT WOS:000307684700013 ER PT J AU Fan, XT Sokorai, KJ Niemira, BA Mills, RS Zhen, MY AF Fan, Xuetong Sokorai, Kimberly J. Niemira, Brendan A. Mills, Robert S. Zhen, Mark Yueqian TI Quality of Gamma Ray-irradiated Iceberg Lettuce and Treatments to Minimize Irradiation-induced Disorders SO HORTSCIENCE LA English DT Article DE modified atmosphere; l-methylcyclopropene; packaging; injury; vein browning; nitrogen flushing; russet spotting ID MODIFIED ATMOSPHERE; PHENOLIC METABOLISM; ETHYLENE; VEGETABLES; RADIATION; FRUITS; 1-METHYLCYCLOPROPENE; INHIBITORS; REDUCTION; RESPONSES AB Irradiation of Iceberg lettuce was recently approved by the U.S. Food and Drug Administration (FDA) to enhance microbial safety and to extend shelf life at doses up to 4 kGy. However, the radiation tolerance of whole head lettuce is unclear. The present study was conducted to investigate the effects of gamma irradiation on the quality of Iceberg lettuce and explore means to reduce irradiation-induced disorders. Irradiation (0.5 and 1.0 kGy) induced symptoms similar to russet spotting and other discolorations (pink ribs, rusty brown, and vein browning) in both external and internal leaves during postirradiation storage in air at 4 degrees C. Irradiation in the absence of oxygen or pretreatment with 1 ppm 1-methylcyclopropene had little effect on the irradiation-induced tissue discoloration. However, low oxygen atmosphere when maintained during the entire 14-day storage almost eliminated the disorders. Compared with non-treated control, irradiation created significantly lower headspace O-2 and higher CO2 levels in the packages, indicating that irradiation promoted respiration. Our results suggest that modified atmosphere (MA) packaging could be used to mitigate irradiation-induced disorders in Iceberg lettuce. C1 [Fan, Xuetong; Sokorai, Kimberly J.; Niemira, Brendan A.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Mills, Robert S.] Tanimura & Antle Fresh Foods Inc, Salinas, CA 93908 USA. [Zhen, Mark Yueqian] AgroFresh Inc, Spring House, PA 19477 USA. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov FU Tanimura and Antle Produce Co. [58-1935-8-882N] FX The study was conducted through a collaborative research agreement with Tanimura and Antle Produce Co. (Agreement No. 58-1935-8-882N). NR 30 TC 1 Z9 1 U1 3 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2012 VL 47 IS 8 BP 1108 EP 1112 PG 5 WC Horticulture SC Agriculture GA 991EQ UT WOS:000307684700022 ER PT J AU Altland, JE Locke, JC AF Altland, James E. Locke, James C. TI Biochar Affects Macronutrient Leaching from a Soilless Substrate SO HORTSCIENCE LA English DT Article DE nitrogen; phosphorus; potassium; fertilizer; columns; growing media ID CHARCOAL; NITRIFICATION; QUANTITY; IMPACT; MANURE; MEDIA AB Byproducts of pyrolysis, known collectively as biochar, are becoming more common and readily available as ventures into alternative energy generation are explored. Little is known about how these materials affect greenhouse container substrates. The objective of this research was to determine the effect of one form of biochar on the nutrient retention and release in a typical commercial greenhouse container substrate. Glass columns filled with 85:15 sphagnum peatmoss:perlite (v:v) and amended with 0%, 1%, 5%, or 10% biochar were drenched with nutrient solution and leached to determine the impact of biochar on nutrient retention and leaching. Nitrate release curves were exponential and peaked lower, at later leaching events, and had higher residual nitrate release over time with increasing biochar amendment rate. This suggests that biochar might be effective in moderating extreme fluctuations of nitrate levels in container substrates over time. Peak phosphate concentration decreased with increasing biochar amendment rate, whereas time of peak release, girth of the peak curve, and final residual phosphate release all increased with increasing biochar amendment. Additional phosphate levels in leachates from biochar-amended substrates, in addition to the higher phosphate concentrations present in later leaching events, suggest this form of biochar as a modest source of phosphate for ornamental plant production. Although there was not sufficient potassium (K) from biochar to adequately replace all fertilizer K in plant production, increasing levels of this form of biochar will add a substantial quantity of K to the substrate and should be accounted for in fertility programs. C1 [Altland, James E.] ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab 27, Wooster, OH 44691 USA. [Locke, James C.] ARS, USDA, Applicat Technol Res Unit, Greenhouse Prod Res Grp, Toledo, OH 43606 USA. RP Altland, JE (reprint author), ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab 27, 1680 Madison Ave, Wooster, OH 44691 USA. EM james.altland@ars.usda.gov NR 20 TC 19 Z9 19 U1 3 U2 72 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2012 VL 47 IS 8 BP 1136 EP 1140 PG 5 WC Horticulture SC Agriculture GA 991EQ UT WOS:000307684700027 ER PT J AU Yin, XH Long, LE Huang, XL Jaja, N Bai, JH Seavert, CF le Roux, J AF Yin, Xinhua Long, Lynn E. Huang, Xiao-Lan Jaja, Ngowari Bai, Jinhe Seavert, Clark F. le Roux, Jac TI Transitional Effects of Double-lateral Drip Irrigation and Straw Mulch on Irrigation Water Consumption, Mineral Nutrition, Yield, and Storability of Sweet Cherry SO HORTTECHNOLOGY LA English DT Article DE Prunus avium; fruit quality; leaf nutrient concentrations; soil microbial communities ID GROUNDCOVER MANAGEMENT-SYSTEMS; DEFICIT IRRIGATION; SOIL; APPLE; TREES; ORCHARD; GROWTH; IMPACTS; QUALITY AB A field trial was conducted on a Cherryhill silt loam soil at The Dalles, OR, from 2006 to 2008. The impacts of switching from the traditional micro sprinkler irrigation (MS) to double-lateral drip irrigation (DD) and from no groundcover with herbicide control of weeds (NC) to in-row wheat (Triticum aestivum) straw mulching (ST) were evaluated in a split-plot design with four replicates. Irrigation water use, mineral nutrition, and productivity of 'Lapins' sweet cherry (Prunus avium) on 'Mazzard' rootstock (P. avium) and soil quality were measured on a plot basis. DD reduced irrigation water consumption by 47.6% to 58.2% compared with MS. Straw mulch lowered irrigation water use by 9.7% relative to NC. Total fruit yield and fruit quality of firmness, size, and sugar at harvest were similar for the irrigation treatments. Straw mulch increased fruit size by 0.6 mm on average relative to NC, which could result in increased grower profitability. The DD system enhanced percentage of marketable fruit by 8.6% relative to MS. Leaf phosphorus (P), boron (B), zinc (Zn), and iron (Fe) concentrations were reduced with DD over MS; consequently, more P, B, Zn, and Fe fertilizers might be needed under DD. Straw mulch markedly decreased the populations of flagellates and amoebae but slightly increased the population of ciliates. Straw mulch resulted in a soil microbial community with remarkably less protozoa. Overall, DD is a viable alternate irrigation system for producing sweet cherry orchards with limited water resources for irrigation. Switching from NC to ST could lower irrigation water use, reduce herbicide runoff, and protect soil from erosion. C1 [Yin, Xinhua; Huang, Xiao-Lan; Jaja, Ngowari] Univ Tennessee, Dept Plant Sci, Jackson, TN 38301 USA. [Long, Lynn E.] Oregon State Univ, Dept Hort, The Dalles, OR 97058 USA. [Bai, Jinhe] ARS, Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Seavert, Clark F.] Oregon State Univ, Dept Agr & Resource Econ, Corvallis, OR 97331 USA. [le Roux, Jac] Irrinet LLC, The Dalles, OR 97058 USA. RP Yin, XH (reprint author), Univ Tennessee, Dept Plant Sci, 605 Airways Blvd, Jackson, TN 38301 USA. EM xyin2@utk.edu OI Huang (huang), Xiao-Lan (xiao lan)/0000-0002-3330-4858 FU USDA NRCS Conservation Innovation Grant Program; Oregon Sweet Cherry Commission; International Plant Nutrition Institute; Oregon State University Agricultural Research Foundation FX This project was supported by USDA NRCS Conservation Innovation Grant Program, Oregon Sweet Cherry Commission, International Plant Nutrition Institute, and Oregon State University Agricultural Research Foundation. NR 35 TC 1 Z9 1 U1 0 U2 16 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2012 VL 22 IS 4 BP 484 EP 492 PG 9 WC Horticulture SC Agriculture GA 993NO UT WOS:000307866600009 ER PT J AU Barnes, J Whipker, B McCall, I Frantz, J AF Barnes, Jared Whipker, Brian McCall, Ingram Frantz, Jonathan TI Nutrient Disorders of 'Evolution' Mealy-cup Sage SO HORTTECHNOLOGY LA English DT Article DE deficiency; macronutrients; micronutrients; Salvia farinacea; toxicity AB To produce floriculture crops like mealy-cup sage (Salvia farinacea), growers must be equipped with cultural information including the ability to recognize and characterize nutrient disorders. 'Evolution' mealy-cup sage plants were grown in silica-sand culture to induce, describe, and photograph symptoms of nutritional disorders. Plants received a complete modified Hoagland's all-nitrate solution of (macronutrient concentrations in millimoles) 15 nitrate-nitrogen (N), 1.0 phosphorus (P), 6.0 potassium (K), 5.0 calcium (Ca), 2.0 magnesium (Mg), and 2.0 sulfur (S) plus (micronutrient concentrations in micromoles) 72 iron (Fe), 18 manganese (Mn), 3 copper (Cu), 3 zinc (Zn), 45 boron (B), and 0.1 molybdenum (Mo). Nutrient-deficient treatments were induced with a complete nutrient formula minus one of the nutrients. The B-toxicity treatment was induced by increasing the element 10-fold higher than the complete nutrient formula. Reagent-grade chemicals and deionized (DI) water of 18 million ohms per centimeter purity were used to formulate treatment solutions. We monitored plants daily to document and photograph sequential series of symptoms as they developed. Typical symptomology of nutrient disorders and corresponding tissue concentrations were determined. Out of 13 treatments, 12 exhibited symptomology; Mo was asymptomatic. Symptoms of N, P, S, Ca, and K deficiencies and B toxicity manifested early; therefore, these disorders may be more likely problems encountered by growers. Unique symptoms were observed on plants grown under N-, Cu-, and Zn-deficient conditions. Necrosis was a common symptom observed, but use of other diagnostic criteria about location on the plant and progression of the disorder can aid growers in diagnosing nutrient disorders of mealy-cup sage. C1 [Barnes, Jared; Whipker, Brian; McCall, Ingram] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Frantz, Jonathan] ARS, USDA, Applicat Technol Res Unit, Toledo, OH 43606 USA. RP Barnes, J (reprint author), N Carolina State Univ, Dept Hort Sci, Box 7609, Raleigh, NC 27695 USA. EM esculentus@gmail.com FU USDA-ARS; Floriculture and Nursery Research Initiative FX We are grateful for the funding support provided by USDA-ARS and the Floriculture and Nursery Research Initiative, and we would like to thank Benary for the mealy-cup sage seed, and to Doug Sturtz, Russ Friedrich, and Alycia Pittenger for nutrient analysis. NR 16 TC 1 Z9 1 U1 1 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2012 VL 22 IS 4 BP 502 EP 508 PG 7 WC Horticulture SC Agriculture GA 993NO UT WOS:000307866600011 ER PT J AU Niedz, RP Hyndman, SE Chellemi, DO Adkins, S AF Niedz, R. P. Hyndman, S. E. Chellemi, D. O. Adkins, S. TI Elimination of Columbian datura virus (CDV) without Thermal Therapy from Brugmansia x candida Pers 'Creamsickle' by In Vitro Culture of Shoot Tips on Medium Containing Ribavirin. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Meeting Abstract CT World Congress on In Vitro Biology CY JUN 03-07, 2012 CL Bellevue, WA SP Soc In Vitro Biol C1 [Niedz, R. P.; Hyndman, S. E.; Chellemi, D. O.; Adkins, S.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM randall.nied-z@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2012 VL 48 IS 4 BP 435 EP 436 PG 2 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 998HC UT WOS:000308227200023 ER PT J AU Freking, BA Purdy, PH Spiller, SF Welsh, CS Blackburn, HD AF Freking, B. A. Purdy, P. H. Spiller, S. F. Welsh, C. S. Blackburn, H. D. TI Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE fertility; ovulation rate; pig; semen quality; uterine capacity ID GENETIC EVALUATION PROGRAM; REPRODUCTIVE TRAITS; SEMEN CHARACTERISTICS; CARCASS TRAITS; ANALYSIS CASA; BODY-WEIGHT; GROWTH; HETEROSIS; SIZE; INSEMINATION AB Selection for 11 generations in swine for ovulation rate (OR) or uterine capacity (UC) resulted in significant changes in component traits of litter size. Our objective was to conserve the unique germplasm for the future and to characterize sperm quality as a correlated response to the selection criterion imposed compared with an unselected control line (CO). Boars representing genetic diversity available in all 3 lines were produced in 2 farrowing seasons. Season 1 was born in September 2005 and was sampled for semen characteristics in October 2006. Season 2 was born in March 2006 and was sampled for semen characteristics in February and March 2007. Each boar (n = 60) was collected twice. The sperm-rich fraction was obtained, and volume and concentration of sperm cells were measured to estimate total sperm production. Each ejaculate was extended 1: 3 (vol/vol) with Androhep Plus (Minitube, Verona, WI) and was packed for shipping to the National Animal Germplasm Program laboratory for processing into frozen straws. Semen quality was measured by computer-assisted semen analysis at 3 semen processing points: fresh (FR), 24 h after extender added (E), and postthaw (PT). A mixed model ANOVA was applied to the data. Fixed effects of farrowing season, line, and 2-way interactions were fitted. The random effect of boar (n = 60) within farrowing season and line was used to test line differences. Sperm concentration was not different (P = 0.18) among the lines (0.594 x 10(9), 0.691 x 10(9), and 0.676 x 10(9) cells/mL for CO, OR, and UC lines, respectively). However, significance (P = 0.04) was detected for the volume of the sperm-rich fraction, greatest for OR (86.4 mL), intermediate for UC (75.5 mL), and least for CO (70.2 mL). Line differences were thus detected (P = 0.02) for total sperm production per ejaculate, greatest for OR (54.9 x 10(9)), intermediate for UC (48.7 x 10(9)), and least for CO (40.5 x 10(9)). A larger percentage of progressively motile sperm and greater estimates of sperm velocity only at processing point E (P < 0.01) were detected in favor of CO. Estimates of motility, velocity, and other parameters of sperm movement measured on E processing points were positively correlated with the same estimates obtained PT, but the magnitude was low to moderate (r range -0.03 to 0.23). Thus, selection for component traits of female reproduction had a favorable effect on total sperm production of boars. C1 [Freking, B. A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Purdy, P. H.; Spiller, S. F.; Welsh, C. S.; Blackburn, H. D.] Natl Anim Germplasm Program, Ft Collins, CO 80521 USA. RP Freking, BA (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM brad.freking@ars.usda.gov NR 36 TC 2 Z9 2 U1 2 U2 14 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2515 EP 2523 DI 10.2527/jas.2012-4723 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400009 PM 22896731 ER PT J AU Walsh, MC Rostagno, MH Gardiner, GE Sutton, AL Richert, BT Radcliffe, JS AF Walsh, M. C. Rostagno, M. H. Gardiner, G. E. Sutton, A. L. Richert, B. T. Radcliffe, J. S. TI Controlling Salmonella infection in weanling pigs through water delivery of direct-fed microbials or organic acids: Part II. Effects on intestinal histology and active nutrient transport SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE direct-fed microbial; intestine; organic acids; pig; Salmonella ID ENTERICA SEROVAR TYPHIMURIUM; ACUTE-PHASE RESPONSE; GROWTH-PERFORMANCE; ESCHERICHIA-COLI; IN-VITRO; SACCHAROMYCES-BOULARDII; WEANED PIGS; SEROTYPE TYPHIMURIUM; ION-TRANSPORT; ZINC-OXIDE AB The objective of this study was to evaluate the effects of water-delivered, direct-fed microbials (DFM) or organic acids on intestinal morphology and active nutrient absorption in weanling pigs after deliberate Salmonella infection. Pigs (n = 88) were weaned at 19 +/- 2 d of age and assigned to 1 of the following treatments, which were administered for 14 d: 1) control diet; 2) control diet + DFM (Enterococcus faecium, Bacillus subtilis, and Bacillus licheniformis) in drinking water at 10(9) cfu/L for each strain of bacteria; 3) control diet + organic acid-based blend (predominantly propionic, acetic, and benzoic acids) in drinking water at 2.58 mL/L; and 4) control diet + 55 mg/kg carbadox. Pigs were challenged with 10(10) cfu Salmonella enterica var Typhimurium 6 d after commencement of treatments. Pigs (n = 22/d) were harvested before Salmonella challenge and on d 2, 4, and 8 after challenge. Duodenal, jejunal, and ileal mucosal tissues were sampled for measurement of villus height and crypt depth. Jejunal tissue was sampled for determination of active nutrient absorption in modified Ussing chambers. Duodenal villus height was greater in pigs fed in-feed antibiotic before infection (P < 0.05). Jejunal crypts were deeper in DFM- and acid-treated pigs on d 4 after infection compared with all other treatments (P < 0.05). Salmonella infection resulted in a linear decrease in phosphorus (P < 0.001) and glucose (P < 0.05) active transport, and an increase (P < 0.001) in glutamine uptake immediately after challenge. Salmonella infection reduced basal short-circuit current (I-sc); however, water-delivered DFM or organic acid treatments caused greater basal Isc on d 2 after challenge than did carbadox. Carbachol-induced chloride ion secretion was greatest in negative control pigs before infection (P < 0.01) and DFM-treated pigs (P < 0.05) after infection. In conclusion, both the DFM and acidification treatments induced increases in basal active ion movement and jejunal crypt depth, which could be interpreted as responses consistent with increased Salmonella pathology, but none of the additives markedly affected intestinal absorptive and secretory function in response to Salmonella challenge. C1 [Walsh, M. C.; Sutton, A. L.; Richert, B. T.; Radcliffe, J. S.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Rostagno, M. H.] USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Gardiner, G. E.] TEAGASC, Moorepk Food Res Ctr, Fermoy, Cork, Ireland. RP Radcliffe, JS (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. EM jradclif@purdue.edu RI walsh, maria/H-3662-2013 FU National Pork Board (Des Moines, IA) FX Financial support provided in part by the National Pork Board (Des Moines, IA). NR 53 TC 7 Z9 7 U1 2 U2 16 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2599 EP 2608 DI 10.2527/jas.2010-3599 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400018 PM 22344321 ER PT J AU Meyer, AM Reed, JJ Neville, TL Taylor, JB Reynolds, LP Redmer, DA Vonnahme, KA Caton, JS AF Meyer, A. M. Reed, J. J. Neville, T. L. Taylor, J. B. Reynolds, L. P. Redmer, D. A. Vonnahme, K. A. Caton, J. S. TI Effects of nutritional plane and selenium supply during gestation on visceral organ mass and indices of intestinal growth and vascularity in primiparous ewes at parturition and during early lactation SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE gastrointestinal tract; gestation; lactation; nutritional plane; selenium; small intestine ID FETAL BODY-WEIGHT; DIETARY RESTRICTION; CELL-PROLIFERATION; GASTROINTESTINAL-TRACT; ANGIOGENIC FACTORS; NUTRIENT RESTRICTION; JEJUNAL VASCULARITY; MATERNAL NUTRITION; ALPHA-AMYLASE; BEEF STEERS AB Objectives were to investigate effects of nutritional plane and Se supply during gestation on visceral organ mass and intestinal growth and vascularization in ewes at parturition and during early lactation. Primiparous Rambouillet ewes (n = 84) were allocated to 2 x 3 x 2 factorial arrangement of treatments. Factors included dietary Se [adequate Se (ASe, 11.5 mu g/kg BW) or high Se (HSe, 77.0 mu g/kg BW)], nutritional plane [60% (restricted; RES), 100% (control; CON), or 140% (high; HIH)], and physiological stage at necropsy (parturition or d 20 of lactation). At parturition, lambs were removed and 42 ewes (7 per treatment) were necropsied. Remaining ewes were transitioned to a common diet which met lactation requirements and mechanically milked for 20 d. In the absence of interactions (P > 0.10), main effects are reported. At parturition, stomach complex and liver masses were greatest for HIH, intermediate for CON, and least for RES (P < 0.02). Small intestinal mass was greater (P <= 0.002) for HIH than RES and CON, and greater (P < 0.01) for ASe than HSe. During early lactation, RES and CON gastrointestinal masses increased disproportionally to BW (P < 0.05). At parturition, jejunal mucosal density was less (P <= 0.01) for RES than CON and HIH, whereas CON had greater (P < 0.003) jejunal mucosal RNA concentration and RNA: DNA than RES and HIH. Although there were no differences (P > 0.17) at parturition, jejunal cell percent proliferation was greatest in RES, intermediate in CON, and least in HIH (P <= 0.09) at d 20 lactation. At both stages, RES had less (P = 0.01) jejunal capillary area density than HIH and less (P <= 0.03) capillary surface density than CON and HIH. During lactation, jejunal capillary size was greater (P = 0.04) for ewes previously fed HSe compared with ASe. At parturition, ASe-HIH had greater (P < 0.02) jejunal mucosal endothelial nitric oxide synthase 3 mRNA than all other treatments and greater (P = 0.10) vascular endothelial growth factor (VEGF) than all treatments, except ASe-RES. In addition, CON had less (P <= 0.08) jejunal VEGF receptor-1 (FLT1) mRNA compared with RES and HIH, and ASe had greater (P = 0.003) FLT1 than HSe at parturition. Ewes fed HIH had greater (P = 0.04) jejunal VEGF receptor-2 mRNA compared with RES. Results indicate that maternal intestinal growth and vascularization are responsive to nutritional plane and dietary Se during gestation and undergo changes postpartum when under similar lactational management. C1 [Meyer, A. M.; Reed, J. J.; Neville, T. L.; Reynolds, L. P.; Redmer, D. A.; Vonnahme, K. A.; Caton, J. S.] N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. [Taylor, J. B.] USDA ARS, US Sheep Expt Stn, Dubois, ID 83423 USA. RP Caton, JS (reprint author), N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. EM joel.caton@ndsu.edu RI Reynolds, Lawrence/I-5267-2015 OI Reynolds, Lawrence/0000-0002-6838-7809 FU USDA-NRI [2003-35206-13621, 2005-35206-15281]; USDA-CSREES; NIH [HL 64141] FX Partially supported by USDA-NRI grants No. 2003-35206-13621 and 2005-35206-15281, from USDA-CSREES, and by NIH Grant HL 64141. Authors thank Roza Yunusova [North Dakota State University (NDSU)] and Lara Woodworth (NDSU) for their assistance with image analysis and the employees of the Animal Nutrition and Physiology Center and Ruminant Nutrition, Physiology, and Muscle Biology Laboratories at NDSU for their contributions to this project. NR 66 TC 11 Z9 11 U1 3 U2 10 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2733 EP 2749 DI 10.2527/jas.2011-4524 PG 17 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400032 PM 22393031 ER PT J AU Puchala, R Animut, G Patra, AK Detweiler, GD Wells, JE Varel, VH Sahlu, T Goetsch, AL AF Puchala, R. Animut, G. Patra, A. K. Detweiler, G. D. Wells, J. E. Varel, V. H. Sahlu, T. Goetsch, A. L. TI Effects of different fresh-cut forages and their hays on feed intake, digestibility, heat production, and ruminal methane emission by Boer x Spanish goats SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE condensed tannins; goats; methane ID CONDENSED TANNINS; IN-VITRO; RUMEN; CATTLE; REQUIREMENTS; PREDICTION; LESPEDEZA; NUTRITION; BACTERIA; HEIFERS AB Twenty-four yearling Boer x Spanish wethers were used to assess effects of different forages, either fresh (Exp. 1) or as hay (Exp. 2), on feed intake, digestibilities, heat production, and ruminal methane emission. Treatments were: 1) Sericea lespedeza (SER; Lespedeza cuneata), a legume high in condensed tannins (CT; 20% and 15% in fresh forage and hay, respectively), 2) SER supplemented with polyethylene glycol (SER-PEG; 25 g/d), 3) alfalfa (Medicago sativa), a legume low in CT (ALF), and 4) sorghumsudangrass (Sorghum bicolor), a grass low in CT (GRASS). Experiments were 22 d, which included 16 d for acclimatization followed by a 6-d period for fecal and urine collection, and gas exchange measurement (last 2 d). Intake of OM was 867, 823, 694, and 691 g/d (SEM = 20.1) with fresh forage, and 806, 887, 681, and 607 g/d with hay for SER, SER-PEG, ALF, and GRASS, respectively (SEM = 46.6). Apparent total tract N digestion was greater for SER-PEG vs. SER (P < 0.001) with fresh forage (46.3%, 66.5%, 81.7%, and 73.2%; SEM = 1.71) and hay (49.7%, 71.4%, 65.4%, and 54.8% for SER, SER-PEG, ALF, and GRASS, respectively; SEM = 1.57). Intake of ME was similar among treatments with fresh forage (8.24, 8.06, 7.42, and 7.70 MJ/d; SEM = 0.434) and with hay was greater for SER-PEG than ALF (P < 0.03) and GRASS (P < 0.001) (8.63, 10.40, 8.15, and 6.74 MJ/d for SER, SER-PEG, ALF, and GRASS, respectively; SEM = 0.655). The number of ciliate protozoa in ruminal fluid was least for SER with fresh forage (P < 0.01) (9.8, 20.1, 21.0, and 33.6 x 10(5)/ml; SEM = 2.76) and hay (P < 0.02) (6.3, 11.4, 13.6, and 12.5 x 10(5)/ml for SER, SER-PEG, ALF, and GRASS, respectively; SEM = 1.43). Methane emission as a percentage of DE intake was lower (P < 0.01) for SER vs. ALF and GRASS with fresh forage (6.6, 8.3, 9.4, and 9.2%; SEM = 0.64) and hay (4.3, 4.9, 6.4, and 6.7% for SER, SER-PEG, ALF, and GRASS, respectively; SEM = 0.38). In summary, methane emission in this short-term experiment was similar between a legume and grass low in CT as fresh forage and hay. The CT in SER markedly decreased N digestibility and elicited a moderate decline in ruminal methane emission. Supplementation with PEG alleviated the effect of CT on N digestibility but not ruminal methane emission, presumably because of different modes of action. In conclusion, potential of using CT-containing forage as a means of decreasing ruminal methane emission requires further study, such as with longer feeding periods. C1 [Puchala, R.; Animut, G.; Patra, A. K.; Detweiler, G. D.; Sahlu, T.; Goetsch, A. L.] Langston Univ, Amer Inst Goat Res, Langston, OK 73050 USA. [Wells, J. E.; Varel, V. H.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Goetsch, AL (reprint author), Langston Univ, Amer Inst Goat Res, Langston, OK 73050 USA. EM goetsch@langston.edu FU USDA [2004-38814-15045] FX This experiment was supported by USDA Project Number 2004-38814-15045. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. USDA is an equal opportunity provider and employer. NR 33 TC 5 Z9 5 U1 3 U2 16 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2754 EP 2762 DI 10.2527/jas.2011-4879 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400034 PM 22408087 ER PT J AU Mulliniks, JT Cox, SH Kemp, ME Endecott, RL Waterman, RC VanLeeuwen, DM Petersen, MK AF Mulliniks, J. T. Cox, S. H. Kemp, M. E. Endecott, R. L. Waterman, R. C. VanLeeuwen, D. M. Petersen, M. K. TI Relationship between body condition score at calving and reproductive performance in young postpartum cows grazing native range SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE average precipitation; beef cattle; body condition score; reproduction ID PRIMIPAROUS BEEF-COWS; PREPARTUM BODY; WEIGHT-GAIN; CATTLE; PARTURITION; ENERGY; NUTRITION; FAT AB Body condition score is used as a management tool to predict competency of reproduction in beef cows. Therefore, a retrospective study was performed to evaluate association of BCS at calving with subsequent pregnancy rate, days to first postpartum ovulation, nutrient status (assessed by blood metabolites), and calf BW change in 2- and 3-yr-old cows (n = 351) managed and selected to fit their environment of grazing native range over 6 yr at the Corona Range and Livestock Research Center, Corona, NM. Cows were managed similarly before calving, without manipulation of management, to achieve predetermined BCS at parturition. Palpable BCS (scale of 1 to 9) were determined by 2 experienced technicians before calving. Cows were classified to 1 of 3 BCS groups prior calving: BCS 4 (mean BCS = 4.3 +/- 0.02), 5 (mean BCS = 5.0 +/- 0.03), or 6 (mean BCS = 5.8 +/- 0.06). Cows were weighed weekly after calving and serum was collected once weekly (1 yr) or twice weekly (5 yr) for progesterone analysis to estimate first postpartum ovulation beginning 35 d postpartum. Year effects also were evaluated, with years identified as either above or below average precipitation. Days to first postpartum ovulation did not differ among calving BCS groups (P = 0.93). Pregnancy rates were not influenced by calving BCS (P = 0.83; 92%, 91%, 90% for BCS 4, 5, and 6, respectively). Days to BW nadir was not influenced by BCS at calving (P = 0.95). Cow BW was different at all measuring points (P < 0.01) with BCS 6 cows having the heaviest BW and cows with BCS 4 the lightest. Cows with calving BCS 4 and 5 lost more (P = 0.06) BW from the initiation of the study to the end of breeding than cows with BCS 6. However, cow BW change at all other measurement periods was not different (P >= 0.49) among calving BCS groups. Serum glucose and NEFA concentrations were not influenced by calving BCS (P >= 0.51). Calf BW at birth (P = 0.60), branding (55-d BW; P = 0.76), and weaning (205-d BW; P = 0.60) were not impacted by cow calving BCS. Body condition score did not influence overall pregnancy rates, indicating that young cows can have a reduced BCS and still be reproductively punctual. Therefore, these results indicate that reproductive performance of young cows with reduced BCS may not be affected when managed in extensive range conditions. C1 [Waterman, R. C.; Petersen, M. K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Mulliniks, J. T.; Cox, S. H.; Kemp, M. E.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Endecott, R. L.] Montana State Univ, Dept Anim & Range Sci, Miles City, MT 59301 USA. [VanLeeuwen, D. M.] New Mexico State Univ, Agr Biometr Serv, Las Cruces, NM 88003 USA. RP Petersen, MK (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM mark.petersen@ars.usda.gov NR 27 TC 14 Z9 16 U1 0 U2 21 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2811 EP 2817 DI 10.2527/jas.2011-4189 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400040 PM 22665663 ER PT J AU Shackelford, SD King, DA Wheeler, TL AF Shackelford, S. D. King, D. A. Wheeler, T. L. TI Chilling rate effects on pork loin tenderness in commercial processing plants SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE chilling; pork; slice shear force; tenderness ID LONGISSIMUS TENDERNESS; SARCOMERE-LENGTH; MUSCLE QUALITY; TECHNICAL NOTE; BEEF; TEMPERATURE; PALATABILITY; POPULATIONS; CALPASTATIN; PROTEOLYSIS AB The present experiment was conducted to provide a large-scale objective comparison of pork LM tenderness and other meat quality traits among packing plants that differ in stunning method and carcass chilling rate. For each of 2 replicates, pigs were sourced from a single barn of a commercial finishing operation that fed pigs from a single terminal crossbred line. On each day, 3 trucks were loaded, with each of those trucks delivering the pigs to a different plant. Plant A used CO2 stunning and conventional spray chilling; Plant B used CO2 stunning and blast chilling; and Plant C used electrical stunning and blast chilling. The boneless, vacuum-packaged loin was obtained from the left side of each carcass (n = 597; 100 . plant(-1) . replicate(-1)). As designed, HCW, LM depth, and LM intramuscular fat percentage did not differ among plants (P > 0.05). By 1.67 h postmortem (1 h after the carcasses exited the harvest floor), the average deep LM temperature was > 10 degrees C warmer for Plant A than Plants B and C (32.1 degrees C, 21.6 degrees C, and 19.3 degrees C, for Plants A, B, and C, respectively) and deep LM temperature continued to be > 10 degrees C warmer for Plant A until 4.17 h or 6.33 h postmortem than for Plants C and B, respectively. Both plants that used blast chilling produced loins with greater LM slice shear force at 15 d postmortem than did the plant that used conventional spray chilling (P < 0.0001). The frequency of loins with excessively high (> 25 kg) LM slice shear force values was greater for Plant B than Plant A (14.7% vs. 1%; P < 0.01). Among all the traits studied, including visual and instrumental evaluations of LM color, ultimate pH, marbling score, and lean color stability, the only other difference between Plants A and B was that purge loss during 13 d (from d 1 to 14) of vacuum-packaged storage was greater for Plant B (P < 0.05). That is, with this sample of pigs and CO2 stunning, no loin quality advantages were detected for blast chilling. Regardless of chilling method, CO2 stunning resulted in darker LM lean color and greater LM water-holding capacity than did electrical stunning (P < 0.05). This research shows that differences in chilling systems among pork packing plants can have a strong influence on loin chop tenderness. C1 [Shackelford, S. D.; King, D. A.; Wheeler, T. L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Shackelford, SD (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM steven.shackelford@ars.usda.gov FU Pork Checkoff FX This project was funded, in part, by The Pork Checkoff. NR 24 TC 7 Z9 7 U1 2 U2 29 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2012 VL 90 IS 8 BP 2842 EP 2849 DI 10.2527/jas.2011-4855 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 995UY UT WOS:000308040400044 PM 22307481 ER PT J AU Li, PY Zhu, JW Qin, YC AF Li, Pengyan Zhu, Junwei Qin, Yuchuan TI Enhanced Attraction of Plutella xylostella (Lepidoptera: Plutellidae) to Pheromone-Baited Traps With the Addition of Green Leaf Volatiles SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE electroantennogram; trap catch; green leaf volatile; Plutella xylostella; sex pheromone ID HOST-PLANT; DIAMONDBACK MOTH; SEX-PHEROMONE; BEHAVIORAL-RESPONSES; SPODOPTERA-EXIGUA; CURCULIONIDAE; COLEOPTERA; ECOLOGY; WEEVIL AB Diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most serious pests of Brassicaceae crops worldwide. Electrophysiological and behavioral responses of P. xylostella to green leaf volatiles (GLVs) alone or together with its female sex pheromone were investigated in laboratory and field. GLVs 1-hexanol and (Z)-3-hexen-1-ol elicited strong electroantennographic responses from unmated male and female P. xylostella, whereas (Z)-3-hexenyl acetate only produced a relatively weak response. The behavioral responses of unmated moths to GLVs were further tested in Y-tube olfactometer experiments. (E)-2-Hexenal, (Z)-3-hexen-1-ol, and (Z)-3-hexenyl acetate induced attraction of males, reaching up to 50%, significantly higher than the response to the unbaited control arm. In field experiments conducted in 2008 and 2009, significantly more moths were captured in traps baited with synthetic sex pheromone with either (Z)-3-hexenyl acetate alone or a blend of (Z)-3-hexenyl acetate, (Z)-3-hexen-1-ol, and (E)-2-hexenal compared with sex pheromone alone and other blend mixtures. These results demonstrated that GLVs could be used to enhance the attraction of P. xylostella males to sex pheromone-baited traps. C1 [Li, Pengyan; Qin, Yuchuan] China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China. [Zhu, Junwei] ARS, Agroecosyst Management Res Unit, USDA, Lincoln, NE 68583 USA. RP Qin, YC (reprint author), China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China. EM qyuchuan@cau.edu.cn FU project 948 Foundation from Ministry of Agriculture of China [2006-G54, 2011-G4]; Professional Science & Technology Foundation from the Ministry of Agriculture of China [200803032, 201103002] FX We thank Prof. Chenzhu Wang (Institute of Zoology, Chinese Academy of Science, Beijing, China) for technical help with EAG recordings. Help from Yanlan Xie and Zhanbin Li in field experiments is appreciated. We thank Brian Wienhold, Fengming Yan, and Wangpeng Shi for comments on the earlier version of this paper. The research was funded by project 948 Foundation (2006-G54, 2011-G4) and the Professional Science & Technology Foundation (200803032, 201103002) from the Ministry of Agriculture of China. NR 25 TC 5 Z9 6 U1 3 U2 40 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1149 EP 1156 DI 10.1603/EC11109 PG 8 WC Entomology SC Entomology GA 996HG UT WOS:000308074900008 PM 22928292 ER PT J AU Villagran, ME Willink, E Vera, MT Follett, P AF Elvira Villagran, M. Willink, Eduardo Teresa Vera, M. Follett, Peter TI Export of Commercial Hass Avocados From Argentina Poses Negligible Risk of Ceratitis capitata (Diptera: Tephritidae) Infestation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mediterranean fruit fly; avocado; host plant resistance; quarantine security; risk analysis ID FRUIT-FLY DIPTERA; SHARWIL AVOCADO; ANASTREPHA-FRATERCULUS; HOST STATUS; NORTHWESTERN ARGENTINA; QUARANTINE TREATMENTS; CONFIDENCE-LIMITS; NONHOST STATUS; SAMPLE-SIZE; OVIPOSITION AB Argentina has to meet quarantine restrictions because of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), to export 'Hass' avocados, Persea americana Miller, to certain countries. Hass avocado at the hard, mature green stage is potentially a conditional nonhost for C. capitata and could open export markets without the need for a quarantine treatment. Trapping data from 1998 to 2006 showed that C. capitata was present in avocado orchards, particularly early in the harvest season. The host status of hard, mature green Hass avocado to C. capitata was evaluated using laboratory and field cage tests under no-choice conditions and by assessing natural levels of infestation in commercially harvested fruit from the main avocado production area. In total, 2,250 hard, mature green avocado fruit were exposed to 11,250 gravid females for 24 or 48h after harvest in laboratory or field cages, and no infestations were found. During 11 seasons, 5,949 fruit in total were sampled from the trees and 992 fruit were collected from the ground, and in none of them were any live or dead fruit fly larvae found. Inspection of >198,000 commercial fruit at the packinghouse from 1998 to 2011 showed no symptoms of fruit fly infestation. These data exceed the published standards for determination of nonhost status, as well as the Probit 9 standard for development of quarantine treatments. Hass avocado harvested at the hard, mature green stage was not infested by C. capitata and seems to pose a negligible quarantine risk. As a consequence, no postharvest treatment or other quarantine actions should be required by importing countries. C1 [Elvira Villagran, M.; Willink, Eduardo; Teresa Vera, M.] Estn Expt Agroind Obispo Colombres EEAOC, San Miguel De Tucuman, Argentina. [Teresa Vera, M.] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina. [Follett, Peter] ARS, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Willink, E (reprint author), Estn Expt Agroind Obispo Colombres EEAOC, San Miguel De Tucuman, Argentina. EM ewillink@eeaoc.org.ar NR 31 TC 0 Z9 0 U1 0 U2 7 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1178 EP 1185 DI 10.1603/EC11369 PG 8 WC Entomology SC Entomology GA 996HG UT WOS:000308074900012 PM 22928296 ER PT J AU Shelly, T Nishimoto, J Kurashima, R AF Shelly, Todd Nishimoto, Jon Kurashima, Rick TI Trap Capture of Three Economically Important Fruit Fly Species (Diptera: Tephritidae): Evaluation of a Solid Formulation Containing Multiple Male Lures in a Hawaiian Coffee Field SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tephritidae; invasive fruit fly; male lure; trapping; surveillance ID BACTROCERA-DORSALIS HENDEL; METHYL EUGENOL; CUE-LURE; FLIES DIPTERA; INSECTICIDE DISPENSERS; LIQUID AB Invasive fruit flies (Diptera: Tephritidae) pose a global threat to agriculture through direct damage to food crops and the accompanying trade restrictions that often result. Early detection is vital to controlling fruit flies, because it increases the probability of limiting the growth and spread of the invasive population and thus may greatly reduce the monetary costs required for eradication or suppression. Male-specific lures are an important component of fruit fly detection, and three such lures are used widely: trimedlure (TML), cue lure (CL), and methyl eugenol (ME), attractive to Mediterranean fruit fly, Ceratitis capitata (Wiedemann); melon fly, Bactrocera cucurbitae (Coquillett); and oriental fruit fly, Bactrocera dorsalis (Hendel), respectively. In California, Florida, and Texas, the two Bactrocera lures are applied to separate species-specific traps as liquids (with a small amount of the insecticide naled added), whereas TML is delivered as a solid plug in another set of traps. Thus, the detection protocol involves considerable handling time as well as potential contact with a pesticide. The purpose of this study was to compare trap capture between liquid male lures and "trilure" wafers that contain TML, ME, raspberry ketone (RK, the hydroxy equivalent of CL), and the toxicant DDVP embedded within a solid matrix. Field studies were conducted in a Hawaiian coffee (Coffea arabica L.) field where the three aforementioned species co-occur, showed that the wafer captured at least as many flies as the liquid baits for all three species. This same result was obtained in comparisons using both fresh and aged (6-wk) baits. Moreover, the wafers performed as well as the single-lure traps in an ancillary experiment in which TML plugs were substituted for liquid TML. Additional experiments demonstrated explicitly that the presence of ME and RK had no effect on captures of C. capitata males and similarly that the presence of TML had no effect on the capture of B. cucurbitae or B. dorsalis males. C1 [Shelly, Todd; Nishimoto, Jon; Kurashima, Rick] USDA APHIS, Waimanalo, HI 96795 USA. RP Shelly, T (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 21 TC 6 Z9 8 U1 0 U2 22 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1186 EP 1193 DI 10.1603/EC11371 PG 8 WC Entomology SC Entomology GA 996HG UT WOS:000308074900013 PM 22928297 ER PT J AU Picard, I Hollingsworth, RG Salmieri, S Lacroix, M AF Picard, Isabelle Hollingsworth, Robert G. Salmieri, Stephane Lacroix, Monique TI Repellency of Essential Oils to Frankliniella occidentalis (Thysanoptera: Thripidae) as Affected by Type of Oil and Polymer Release SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE essential oil; polymer; coating; thrip; Frankliniella occidentalis ID WESTERN FLOWER THRIPS; PLANT ESSENTIAL OILS; PEST-MANAGEMENT; ALGINATE; PERGANDE; ORCHIDS; PALMI; STRATEGIES; CHITOSAN; LIMONENE AB Eight essential oils [0.125-1.0% (vol: vol) in acetone] were separately deposited on leaf disks to evaluate their potential to repel western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), adult females. Two of the best-performing essential oils were incorporated into polymer matrices of methyl cellulose or alginate [0.5 or 1% (wt: vol)] to verify the potential of the polymer to extend repellency of oils over time (24-120 h). Results showed that at a concentration of 0.5%, Thymus vulgaris L. (common thyme) and Satureja montana L. (winter savory) were the most repellent essential oils. For these two treatments, no western flower thrips were counted on treated leaf disks 60 min after the start of the test. T. serpyllum and O. compactum also showed repellency values >= 90% at this concentration. With both the alginate and methyl cellulose polymers, the incorporation of polymers into treatment solutions containing 0.5% concentrations of S. montana and T. serpyllum resulted in higher repellency compared with treatment solutions lacking these polymers for a minimum of 3 d. For the alginate polymer, differences associated with polymer concentrations were most dramatic. High repellency was maintained for 4 d when a 0.5% concentration of the alginate was used in combination with a 0.5% concentration of S. montana. The use of repellent oils with polymers that extend their repellency may prove useful for both pre- and postharvest applications in flower crops. C1 [Hollingsworth, Robert G.] ARS, US Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. [Picard, Isabelle; Salmieri, Stephane; Lacroix, Monique] Canadian Irradiat Ctr, Res Labs Sci Appl Food, INRS Inst Armand Frappier, Laval, PQ H7V 1B7, Canada. RP Hollingsworth, RG (reprint author), ARS, US Pacific Basin Agr Res Ctr, USDA, 64 Nowelo St, Hilo, HI 96720 USA. EM robert.hollingsworth@ars.usda.gov FU Minister of Economic Development, Innovation and Export Trade, International Program (MDEIE); U.S. Pacific Basin Agricultural Research Center; Foundation Armand-Frappier; Fund for Nature Research and Technology: International Training Program FX We thank Bruce Mackey for advice on statistical analysis. We are also grateful to USDA-ARS scientists Joseph Patt (Weslaco, TX) and Stuart Reitz (Tallahassee, FL) for reviewing an earlier draft of this manuscript. This research was supported by the Minister of Economic Development, Innovation and Export Trade, International Program (MDEIE) and by the U.S. Pacific Basin Agricultural Research Center. I.P. was supported by a fellowship from the Foundation Armand-Frappier and the Fund for Nature Research and Technology: International Training Program. NR 44 TC 7 Z9 8 U1 1 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1238 EP 1247 DI 10.1603/EC11292 PG 10 WC Entomology SC Entomology GA 996HG UT WOS:000308074900019 PM 22928303 ER PT J AU Zukoff, SN Bailey, WC Ellersieck, MR Hibbard, BE AF Zukoff, Sarah N. Bailey, Wayne C. Ellersieck, Mark R. Hibbard, Bruce E. TI Western Corn Rootworm Larval Movement in SmartStax Seed Blend Scenarios SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE maize; Bt maize; pyramid; nontarget; Diabrotica virgifera virgifera ID DIABROTICA-VIRGIFERA-VIRGIFERA; EVENT DAS-59122-7 MAIZE; TO-PLANT MOVEMENT; COLEOPTERA-CHRYSOMELIDAE; TRANSGENIC-MAIZE; LECONTE COLEOPTERA; ESTABLISHMENT; MISSOURI; SURVIVAL; ROOTS AB Insect resistance management (IRM) can extend the lifetime of management options, but depends on extensive knowledge of the biology of the pest species involved for an optimal plan. Recently, the Environmental Protection Agency (EPA) registered seed blends refuge for two of the transgenic Bacillus thuringiensis (Bt) corn products targeting the western corn rootworm, Diabrotica virgifera virgifera LeConte. Larval movement between Bt and isoline plants can be detrimental to resistance management for high dose Bt products because the larger larvae can be more tolerant of the Bt toxins. We assessed movement of western corn rootworm larvae among four spatial arrangements of SmartStax corn (expressing both the Cry34/35Ab1 and Cry3Bb1 proteins) and isoline plants by infesting specific plants with wild type western corn rootworm eggs. Significantly fewer western corn rootworm larvae, on average, were recovered from infested SmartStax plants than infested isoline plants, and the SmartStax plants were significantly less damaged than corresponding isoline plants. However, when two infested isoline plants surrounded a SmartStax plant, a significant number of larvae moved onto the SmartStax plant late in the season. These larvae caused significant damage both years and produced significantly more beetles than any other plant configuration in the study (including isoline plants) in the first year of the study. This plant configuration would occur rarely in a 5% seed blend refuge and may produce beetles of a susceptible genotype because much of their initial larval development was on isoline plants. Results are discussed in terms of their potential effects on resistance management. C1 [Hibbard, Bruce E.] Univ Missouri, USDA, ARS, Columbia, MO 65211 USA. [Zukoff, Sarah N.; Bailey, Wayne C.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ellersieck, Mark R.] Univ Missouri, Agr Expt Stn, Columbia, MO 65211 USA. RP Hibbard, BE (reprint author), Univ Missouri, USDA, ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM bruce.hibbard@ars.usda.gov FU USDA-CSREES [2009-35302-05256]; USDA-ARS; Monsanto Company via USDA-ARS Trust [58-3622-0-440] FX We thank Matt Higdon, Julie Barry, Anthony Zukoff, Tim Praiswater, and Daniel Frank (all USDA-ARS, Columbia, MO) and a number of temporary laborers for technical assistance. We thank Deborah Finke (University of Missouri) and Stefan Toepfer (CABI Europe, Hodmezovasarhely, Hungary) for helpful editing suggestions. We thank Monsanto Company for providing seed and EnviroLogix gene check strips. Funding, in part, was provided by USDA-CSREES Agreement No. 2009-35302-05256, USDA-ARS, and Monsanto Company via USDA-ARS Trust Fund Cooperative Agreement No. 58-3622-0-440. NR 41 TC 11 Z9 11 U1 1 U2 34 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1248 EP 1260 DI 10.1603/EC12031 PG 13 WC Entomology SC Entomology GA 996HG UT WOS:000308074900020 PM 22928304 ER PT J AU Goolsby, JA Adamczyk, JJ Crosslin, JM Troxclair, NN Anciso, JR Bester, GG Bradshaw, JD Bynum, ED Carpio, LA Henne, DC Joshi, A Munyaneza, JE Porter, P Sloderbeck, PE Supak, JR Rush, CM Willett, FJ Zechmann, BJ Zens, BA AF Goolsby, J. A. Adamczyk, J. J., Jr. Crosslin, J. M. Troxclair, N. N. Anciso, J. R. Bester, G. G. Bradshaw, J. D. Bynum, E. D. Carpio, L. A. Henne, D. C. Joshi, A. Munyaneza, J. E. Porter, P. Sloderbeck, P. E. Supak, J. R. Rush, C. M. Willett, F. J. Zechmann, B. J. Zens, B. A. TI Seasonal Population Dynamics of the Potato Psyllid (Hemiptera: Triozidae) and Its Associated Pathogen "Candidatus Liberibacter solanacearum" in Potatoes in the Southern Great Plains of North America SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bactericera cockerelli; zebra chip; Liberibacter; insect vector; areawide pest management ID ZEBRA CHIP DISEASE; BACTERICERA-COCKERELLI SULC; 1ST REPORT; INSECTICIDES; TRANSMISSION; PCR; TOMATO; MEXICO; PLANTS; CROPS AB The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), and its associated pathogen "Candidatus Liberibacter solanacearum" (Ca. L. solanacearum), the putative causal agent of zebra chip (ZC) disease in potatoes (Solanum tuberosum L.), were sampled in commercial potato fields and untreated control plots for 3 yr in multiple locations in Texas, Kansas, Nebraska, and Colorado. Populations of the potato psyllid varied across years and across potato growing regions. However, the percentage of potato psyllids infected with Ca. L. solanacearum although variable across years, was consistently highest in the Lower Rio Grande Valley of Texas (LRGV), the reported overwintering location for this pest. The numbers of Ca. L. solanacearum-infected psyllids collected on field traps and large nymphs counted on leaf samples were both positively correlated with the final percentage of ZC in tubers. In the LRGV, where vector and disease pressure is the highest, population levels of immature life stages of the psyllid and percentage of ZC differed greatly between commercial and untreated fields. These results show that the pest management program that was used can be effective at controlling development of the psyllid and ultimately reducing the incidence of ZC. C1 [Goolsby, J. A.; Adamczyk, J. J., Jr.] ARS, USDA, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Crosslin, J. M.] ARS, USDA, Prosser, WA 99350 USA. [Troxclair, N. N.] Texas Agrilife Extens, Uvalde, TX 78801 USA. [Anciso, J. R.] Texas Agrilife Extens, Weslaco, TX 78696 USA. [Bester, G. G.] Frito Lay, Rhinelander, WI 54501 USA. [Bradshaw, J. D.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Bynum, E. D.] Texas Agrilife Res, Amarillo, TX 79106 USA. [Carpio, L. A.; Zechmann, B. J.; Zens, B. A.] CSS Farms, Minden, NE 69361 USA. [Carpio, L. A.; Zechmann, B. J.; Zens, B. A.] CSS Farms, Dalhart, TX 79022 USA. [Henne, D. C.; Sloderbeck, P. E.] Texas Agrilife Res, Weslaco, TX 78596 USA. [Joshi, A.; Munyaneza, J. E.] K State Extens, Garden City, KS 67846 USA. [Porter, P.] Texas Agrilife Extens, Lubbock, TX 79403 USA. [Supak, J. R.] Texas Agrilife Res, College Stn, TX 77843 USA. [Rush, C. M.] Texas Agrilife Res, Bushland, TX 79106 USA. [Willett, F. J.] Agro Engn, Alamosa, CO 81101 USA. RP Goolsby, JA (reprint author), ARS, USDA, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM john.goolsby@ars.usda.gov FU USDA-ARS; USDA-NIFA Specialty Crop Research Initiative; Texas Department of Agriculture/Texas Agrilife Zebra Chip Program; Frito Lay; U.S. potato growers FX We thank Bill Warfield, Reyes Garcia, Jennifer Plata, Will Summers, Jacqueline Valencia, Polo Garza, and Andy Cruz (ARS-Weslaco) for technical assistance; Launa Hamlin, Eric Krohn, and Mary Roster for testing the insect and plant samples for putative plant pathogens; and Douglas Miller (USDAD-ARS-SEL) for identification of the psyllids. We also thank the many potato growers who enthusiastically participated in the research: Jack Wallace, Wallace Farms, Edinburg, TX; John Nordgaard, Black Gold Farms, Pearsall, TX; Mark Lynch, Premium Source, Pearsall, TX and Garden City, KS; Milt Carter and John Wallace, CSS Farms, Olton and Dalhart, TX; Bruce and Steve Barrett, Barrett Farms, Springlake, TX; Brian Walther, Walther Farms, Scottsbluff, NE, and Wray, CO; Jim Allen, Western Farms, Bridgeport, NE; Jon Gilley, R. D. Offutt Farms, O'Neil, NE; and Charlie Higgins, Farmington, NM. We thank Drs. Don Thomas and Scott Armstrong (USDA-ARS, Weslaco, TX) for helpful reviews of the manuscript. This study was supported by USDA-ARS, USDA-NIFA Specialty Crop Research Initiative, Texas Department of Agriculture/Texas Agrilife Zebra Chip Program, Frito Lay, and the U.S. potato growers who provided matching funding. NR 43 TC 21 Z9 22 U1 3 U2 26 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1268 EP 1276 DI 10.1603/EC11435 PG 9 WC Entomology SC Entomology GA 996HG UT WOS:000308074900022 PM 22928306 ER PT J AU Lee, JC Burrack, HJ Barrantes, LD Beers, EH Dreves, AJ Hamby, KA Haviland, DR Isaacs, R Richardson, TA Shearer, PW Stanley, CA Walsh, DB Walton, VM Zalom, FG Bruck, DJ AF Lee, Jana C. Burrack, Hannah J. Barrantes, Luz D. Beers, Elizabeth H. Dreves, Amy J. Hamby, Kelly A. Haviland, David R. Isaacs, Rufus Richardson, Tamara A. Shearer, Peter W. Stanley, Cory A. Walsh, Doug B. Walton, Vaughn M. Zalom, Frank G. Bruck, Denny J. TI Evaluation of Monitoring Traps for Drosophila suzukii (Diptera: Drosophilidae) in North America SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Drosophila suzukii; detection; monitoring; trapping AB Drosophila suzukii Matsumura (Diptera: Drosophilidae), a recent invasive pest of small and stone fruits, has been detected in more than half of the U. S. states, and in Canada, Mexico, and Europe. Upon discovery, several different trap designs were recommended for monitoring. This study compared the trap designs across seven states/provinces in North America and nine crop types. Between May and November 2011, we compared a clear cup with 10 side holes (clear); a commercial trap with two side holes (commercial); a Rubbermaid container with mesh lid and rain tent (Haviland), and with 10 side holes and no tent (modified Haviland); a red cup with 10 side holes (red); and a white container with mesh lid and rain tent (Van Steenwyk). Although fly catches among traps varied per site, overall, the Haviland trap caught the most D. suzukii, followed by the red, Van Steenwyk, and clear trap. The modified Haviland and commercial trap had low captures. Among five crop types in Oregon, a clear cup with mesh sides (Dreves) also was tested and caught the most flies. Traps with greater entry areas, found in mesh traps, caught more flies than traps with smaller entry areas. In terms of sensitivity and selectivity, traps that caught more flies likewise caught flies earlier, and all traps caught 26-31% D. suzukii out of the total Drosophila captured. Future trap improvements should incorporate more entry points and focus on selective baits to improve efficiency and selectivity with regard to the seasonal behavior of D. suzukii. C1 [Lee, Jana C.; Bruck, Denny J.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Burrack, Hannah J.; Walsh, Doug B.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Barrantes, Luz D.] Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Beers, Elizabeth H.] Washington State Univ, Ctr Tree Fruit Res & Extens, Wenatchee, WA 98801 USA. [Dreves, Amy J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Hamby, Kelly A.; Zalom, Frank G.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Haviland, David R.] Univ Calif Cooperat Extens, Bakersfield, CA 93307 USA. [Isaacs, Rufus] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Richardson, Tamara A.] Univ No British Columbia, Prince George, BC V2Z 4N9, Canada. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, Hood River, OR 97031 USA. [Stanley, Cory A.] Utah State Univ, Dept Biol, Utah State Univ Extens, Logan, UT 84322 USA. [Walton, Vaughn M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Lee, JC (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM jana.lee@ars.usda.gov FU USDA Specialty Crops Research Initiative [2010-51181-21167]; USDAD CRIS [5358-22000-032-00D]; Michigan Department of Agriculture Specialty Crop Block grant; MBG Marketing FX We thank Adam Cave, Dave Edwards, Amelia Thornhill, and Bev Thomas for constructing traps; Bruce Mackey for statistical assistance; and Joe Kleiber for comments. We thank Preston Brown, Dave Edwards, Clark Evans, Rich Little, Merrill Longmore, Laura Machial, Amanda Ohrn, Wyatt Rice-Narusch, Stephanie Rill, Heather Wilson, Steve Van Timmeren, Sarah Wong, and Doris Yu for field assistance. This project was funded by USDA Specialty Crops Research Initiative grant 2010-51181-21167, USDAD CRIS 5358-22000-032-00D, a Michigan Department of Agriculture Specialty Crop Block grant, and MBG Marketing. NR 13 TC 33 Z9 34 U1 1 U2 92 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1350 EP 1357 DI 10.1603/EC12132 PG 8 WC Entomology SC Entomology GA 996HG UT WOS:000308074900032 PM 22928316 ER PT J AU Prabhaker, N Gispert, C Castle, SJ AF Prabhaker, Nilima Gispert, Carmen Castle, Steven J. TI Baseline Susceptibility of Planococcus ficus (Hemiptera: Pseudococcidae) From California to Select Insecticides SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE resistance monitoring; discriminating dose; Planococcus ficus; immature; imidacloprid ID WHITEFLY HOMOPTERA; VINE MEALYBUG; RESISTANCE; CICADELLIDAE; ALEYRODIDAE; BUPROFEZIN; VINEYARDS AB Between 2006 and 2008, 20 populations of Planococcus ficus (Signoret), from Coachella and San Joaquin Valleys of California were measured in the laboratory for susceptibility to buprofezin, chlorpyrifos, dimethoate, methomyl, and imidacloprid. Toxicity was assessed using a petri dish bioassay technique for contact insecticides and by a systemic uptake technique for imidacloprid. Mixed life stages were tested for susceptibility to all insecticides except for buprofezin, which was measured against early and late instars (first, second, and third). Dose-response regression lines from the mortality data established LC50 and LC99 values by both techniques. Responses of populations from the two geographical locations to all five insecticides varied, in some cases significantly. Variations in susceptibility to each insecticide among sample sites showed a sevenfold difference for buprofezin, 11-fold to chlorpyrifos, ninefold to dimethoate, 24-fold to methomyl, and 8.5-fold to imidacloprid. In spite of susceptibility differences between populations, baseline toxicity data revealed that all five insecticides were quite effective based on low LC(50)s. Chlorpyrifos was the most toxic compound to Planococcus ficus populations as shown by lowest LC(50)s. Buprofezin was toxic to all immature stages but was more potent to first instars. The highest LC99 estimated by probit analysis of the bioassay data of all 20 populations for each compound was selected as a candidate discriminating dose for use in future resistance monitoring efforts. Establishment of baseline data and development of resistance monitoring tools such as bioassay methods and discriminating doses are essential elements of a sustainable management program for Planococcus ficus. C1 [Castle, Steven J.] ARS, ALARC, USDA, Maricopa, AZ 85239 USA. [Prabhaker, Nilima] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Gispert, Carmen] Univ Calif Cooperat Extens, Indio, CA 92201 USA. RP Castle, SJ (reprint author), ARS, ALARC, USDA, Maricopa, AZ 85239 USA. EM steven.castle@ars.usda.gov FU Exotic/Invasive Pests and Diseases Research Program FX We thank Paul Merten for valuable technical assistance. This study was funded in part by Exotic/Invasive Pests and Diseases Research Program. NR 32 TC 2 Z9 2 U1 3 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1392 EP 1400 DI 10.1603/EC11340 PG 9 WC Entomology SC Entomology GA 996HG UT WOS:000308074900037 PM 22928321 ER PT J AU Petzold-Maxwell, JL Cibils-Stewart, X French, BW Gassmann, AJ AF Petzold-Maxwell, Jennifer L. Cibils-Stewart, Ximena French, B. Wade Gassmann, Aaron J. TI Adaptation by Western Corn Rootworm (Coleoptera: Chrysomelidae) to Bt Maize: Inheritance, Fitness Costs, and Feeding Preference SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; feeding behavior; pest resistance; transgenic maize ID VIRGIFERA-VIRGIFERA COLEOPTERA; PINK-BOLLWORM LEPIDOPTERA; HELIOTHIS-VIRESCENS LEPIDOPTERA; EVENT DAS-59122-7 MAIZE; BACILLUS-THURINGIENSIS; INSECT RESISTANCE; TRANSGENIC-MAIZE; ENTOMOPATHOGENIC NEMATODES; LARVAL SUSCEPTIBILITY; TOXIN CRY1AC AB We examined inheritance of resistance, feeding behavior, and fitness costs for a laboratory-selected strain of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), with resistance to maize (Zea maize L.) producing the Bacillus thuringiensis Berliner (Bt) toxin Cry3Bb1. The resistant strain developed faster and had increased survival on Bt maize relative to a susceptible strain. Results from reciprocal crosses of the resistant and susceptible strains indicated that inheritance of resistance was nonrecessive. No fitness costs were associated with resistance alleles in the presence of two entomopathogenic nematode species, Steinernema carpocapsae Weiser and Heterorhabditis bacteriophora Poinar. Larval feeding studies indicated that the susceptible and resistant strains did not differ in preference for Bt and non-Bt root tissue in choice assays. C1 [Petzold-Maxwell, Jennifer L.; Cibils-Stewart, Ximena; Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [French, B. Wade] USDA ARS, N Cent Agr Res Lab, Brookings, SD 57006 USA. RP Petzold-Maxwell, JL (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM jpetzold@iastate.edu FU U.S. Department of Agriculture [2009-33120-20256, 2010-33522-21673] FX We thank L. Momberg, S. Bradley, and A. Hoffmann for laboratory assistance. This research was supported through Biotechnology Risk Assessment grants 2009-33120-20256 and 2010-33522-21673 from the U.S. Department of Agriculture. NR 68 TC 22 Z9 23 U1 0 U2 66 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1407 EP 1418 DI 10.1603/EC11425 PG 12 WC Entomology SC Entomology GA 996HG UT WOS:000308074900039 PM 22928323 ER PT J AU Bansal, R Mamidala, P Mian, MAR Mittapalli, O Michel, AP AF Bansal, Raman Mamidala, Praveen Mian, M. A. Rouf Mittapalli, Omprakash Michel, Andy P. TI Validation of Reference Genes for Gene Expression Studies in Aphis glycines (Hemiptera: Aphididae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE housekeeping gene; quantitative real-time polymerase chain reaction; normalization; Aphis glycines ID REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; SOYBEAN APHID; NORTH-AMERICA; ANNOTATION; SELECTION; TISSUES; MANAGEMENT; CHALLENGE; BLAST2GO AB Quantitative real-time polymerase chain reaction (qRT-PCR) is a common and robust tool for accurate quantification of mRNA transcripts. To normalize results, a housekeeping gene ([HKG], reference gene or endogenous control gene) is mandatory. Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a significant soybean, Glycine max (L.) Merr., pest, yet gene expression and functional genomics studies are hindered by a lack of stable HKGs. We evaluated seven potential HKGs (SDFS, succinate dehydrogenase flavoprotein subunit; EF1a, elongation factor-1 alpha; HEL, helicase; GAPDH, glyceraldehyde-3 phosphate dehydrogenase; RPS9, ribosomal protein S9; TBP, TATA-box binding protein; and UBQ, ubiquitin-conjugating protein) to determine the most efficient HKGs that have stable expression among tissues, developmental stages, and aphids fed on susceptible and host plant-resistant soybean. HKG stability was determined using GeNorm and NormFinder. Results from three different experimental conditions revealed high stability of TBP compared with the other HKGs profiled across the samples assayed. RPS9 showed stable expression among aphids on susceptible and resistant plants, whereas EF1a showed stable expression in tissues and developmental stages. Therefore, we recommend the TBP as a suitable HKG for efficient normalization among treatments, tissues, and developmental stages of A. glycines. In addition, RPS9 may be used for host-plant resistance experiments and EF1a could be considered for testing differential expression across tissues or developmental stages. These results will enable a more accurate and reliable normalization of qRT-PCR data in A. glycines. C1 [Bansal, Raman; Mamidala, Praveen; Mittapalli, Omprakash; Michel, Andy P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. Rouf] USDA ARS, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Michel, AP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM michel.70@osu.edu FU North Central Soybean Research Program; Ohio Soybean Council [11-2-04]; Department of Entomology; Ohio Agricultural Research and Development Center FX This work was supported by a grant from North Central Soybean Research Program and The Ohio Soybean Council (11-2-04). The CFX-96 thermocycler system was purchased, in part, by an equipment grant from Department of Entomology and Ohio Agricultural Research and Development Center. NR 34 TC 20 Z9 25 U1 6 U2 27 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1432 EP 1438 DI 10.1603/EC12095 PG 7 WC Entomology SC Entomology GA 996HG UT WOS:000308074900042 PM 22928326 ER PT J AU Srinivasan, R Riley, D Diffie, S Sparks, A Adkins, S AF Srinivasan, Rajagopalbabu Riley, David Diffie, Stan Sparks, Alton Adkins, Scott TI Whitefly Population Dynamics and Evaluation of Whitefly-Transmitted Tomato Yellow Leaf Curl Virus (TYLCV)-Resistant Tomato Genotypes as Whitefly and TYLCV Reservoirs SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE vector; Begomovirus; transmission; host plant resistance ID BEMISIA-TABACI; 1ST REPORT; BIOTYPE-B; GEMINIVIRUSES; RESISTANCE; TRANSMISSION; HOST; ACQUISITION; WORLDWIDE; FLORIDA AB Sweetpotato whitefly, Bemisia tabaci (Gennadius), and whitefly-transmitted tomato yellow leaf curl virus (TYLCV) are major threats to tomato production in the southeastern United States. TYLCV was introduced to Florida from the Caribbean islands and has spread to other southern states of the United States. In Georgia, in recent years, the incidence of TYLCV has been steadily increasing. Studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of Georgia, to evaluate TYLCV-resistant genotypes against whiteflies and TYLCV, and to assess the potential role of resistant genotypes in TYLCV epidemiology. Monitoring studies indicated that the peak incidence of whiteflies varied seasonally from year to year. In general, whitefly populations were not uniformly distributed. Tomato genotypes exhibited minor differences in their ability to support whitefly populations. TYLCV symptoms were visually undetectable in all but one resistant genotype. The infection rates (visually) in susceptible genotypes ranged from 40 to 87%. Greenhouse inoculations with viruliferous whiteflies followed by polymerase chain reaction (PCR) indicated that up to 100% of plants of resistant genotypes were infected, although predominantly symptomless. TYLCV acquisition by whiteflies from TYLCV-infected genotypes was tested by PCR; TYLCV acquisition rates from resistant genotypes were less than from susceptible genotypes. Nevertheless, this difference did not influence TYLCV transmission rates from resistant to susceptible genotypes. Results emphasize that resistant genotypes can serve as TYLCV and whitefly reservoirs and potentially influence TYLCV epidemics. C1 [Srinivasan, Rajagopalbabu; Riley, David; Diffie, Stan; Sparks, Alton] Univ Georgia, Dept Entomol, Tifton, GA 31793 USA. [Adkins, Scott] USDA ARS, Ft Pierce, FL 34945 USA. RP Srinivasan, R (reprint author), Univ Georgia, Dept Entomol, 2360 Rainwater Rd, Tifton, GA 31793 USA. EM babusri@uga.edu FU Southern Region IPM USDA-CSREES; Georgia Commodity Commission for Vegetables FX The authors express their sincere thanks to Simeon Mckeown and Sheran Thompson for their technical assistance. The authors acknowledge the partial funding received from Southern Region IPM USDA-CSREES and Georgia Commodity Commission for Vegetables. The statistical assistance provided by Jerry Davis, University of Georgia, Griffin Campus is greatly appreciated. NR 36 TC 10 Z9 10 U1 1 U2 33 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1447 EP 1456 DI 10.1603/EC11402 PG 10 WC Entomology SC Entomology GA 996HG UT WOS:000308074900044 PM 22928328 ER PT J AU Ni, XZ Xu, WW Blanco, MH Wilson, JP AF Ni, Xinzhi Xu, Wenwei Blanco, Michael H. Wilson, Jeffrey P. TI Evaluation of Corn Germplasm Lines for Multiple Ear-Colonizing Insect and Disease Resistance SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE corn earworm; maize weevil; stink bug-discolored kernel; common smut; principal components analysis ID EARWORM LEPIDOPTERA; SILK MAYSIN; AFLATOXIN CONTAMINATION; NOCTUIDAE LARVAE; MAIZE GERMPLASM; INBRED LINES; INJURY; GEORGIA; HYBRIDS; GRAIN AB Ear-colonizing insects and diseases that reduce yield and impose health threats by mycotoxin contaminations in the grain, are critical impediments for corn (Zea mays L.) production in the southern United States. Ten germplasm lines from the Germplasm Enhancement of Maize (GEM) Program in Ames, IA, and Raleigh, NC, and 10 lines (derived from GEM germplasm) from the Texas Agricultural Experiment Station in Lubbock, TX, were examined in 2007 and 2008 with local resistant and susceptible controls. Four types of insect damage and smut disease (Ustilago maydis) infection, as well as gene x environment (G x E) interaction, was assessed on corn ears under field conditions. Insect damage on corn ears was further separated as cob and kernel damage. Cob penetration rating was used to assess corn earworm [Helicoverpa zea (Boddie)] and fall armyworm [Spodoptera frugiperda (J.E. Smith)] feeding on corn cobs, whereas kernel damage was assessed using three parameters: 1) percentage of kernels discolored by stink bugs (i.e., brown stink bug [Euschistus servus (Say)], southern green stink bug [Nezara viridula (L.)], and green stink bug [Chinavia (Acrosternum) hilare (Say)]; 2) percentage of maize weevil (Sitophilus zeamais Motschulsky) - damaged kernels; and 3) percentage of kernels damaged by sap beetle (Carpophilus spp.), "chocolate milkworm" (Moodna spp.), and pink scavenger caterpillar [Pyroderces (Anatrachyntis) rileyi (Walsingham)]. The smut infection rates on ears, tassels, and nodes also were assessed. Ear protection traits (i.e., husk tightness and extension) in relation to insect damage and smut infection also were examined. Significant differences in insect damage, smut infection, and husk protection traits were detected among the germplasm lines. Three of the 20 germplasm lines were identified as being multiple insect and smut resistant. Of the three lines, entries 5 and 7 were derived from DKXL370, which was developed using corn germplasm from Brazil, whereas entry 14 was derived from CUBA117. C1 [Ni, Xinzhi; Wilson, Jeffrey P.] Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Xu, Wenwei] Texas A&M Univ, Ctr Agr Res & Extens, College Stn, TX 79403 USA. [Blanco, Michael H.] Iowa State Univ, USDA ARS, Plant Intro Res Unit, Ames, IA 50011 USA. RP Ni, XZ (reprint author), Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. EM xinzhi.ni@ars.usda.gov NR 31 TC 7 Z9 7 U1 3 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1457 EP 1464 DI 10.1603/EC12115 PG 8 WC Entomology SC Entomology GA 996HG UT WOS:000308074900045 PM 22928329 ER PT J AU Lightle, DM Dossett, M Backus, EA Lee, JC AF Lightle, D. M. Dossett, M. Backus, E. A. Lee, J. C. TI Location of the Mechanism of Resistance to Amphorophora agathonica (Hemiptera: Aphididae) in Red Raspberry SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE electrical penetration graph; plant resistance; feeding behavior; antixenosis ID BLACK-RASPBERRY; IDENTIFICATION; IMMUNITY; BEHAVIOR; VIRUS AB The aphid Amphorophora agathonica Hottes (Hemiptera: Aphididae) is an important virus vector in red (Rubus idaeus L.) and black (Rubus occidentalis L.) raspberries in North America. Raspberry resistance to A. agathonica in the form of a single dominant gene named Ag-1 has been relied upon to help control aphid-transmitted plant viruses; however, the mechanism of resistance to the insect is poorly understood. Aphid feeding was monitored using an electrical penetration graph on the resistant red raspberry 'Tulameen' and compared with a susceptible control, 'Vintage'. There were no differences in pathway feeding behaviors of aphids as they moved toward the phloem. Once in the phloem, however, aphids feeding on resistant plants spent significantly more time salivating than on susceptible plants, and ingested significantly less phloem sap. This suggests that a mechanism for resistance to A. agathonica is located in the phloem. Reduced ingestion of phloem may result in inefficient acquisition of viruses and is a likely explanation for the lack of aphid-transmitted viruses in plantings of resistant cultivars. C1 [Lightle, D. M.] Oregon State Univ, Corvallis, OR 97330 USA. [Dossett, M.] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Backus, E. A.] USDA ARS, Crop Dis Pests & Genet Res Unit, Parlier, CA 93619 USA. [Lee, J. C.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97331 USA. RP Lightle, DM (reprint author), Oregon State Univ, 4017 Ag & Life Sci Bldg, Corvallis, OR 97330 USA. EM lightled@onid.orst.edu FU USDA Specialty Crops Research Initiative [2009-51181-06022, CRIS 5358-22000-032-00D] FX We thank M. Stamm (University of Nebraska) and an anonymous reviewer for comments on the manuscript, N. Mosier (USDA-ARS HCRU) for testing the plant clones for aphid-vectored viruses, Sakuma Bros. for kindly donating plant material, P. Chen (Noldus Information Technology) for interfacing the Observer program with WinDaq, and J. Sears (USDA-ARS HCRU) for installing a dedicated electrical line into the laboratory. Funding was provided by a USDA Specialty Crops Research Initiative grant 2009-51181-06022, and CRIS 5358-22000-032-00D. NR 30 TC 3 Z9 3 U1 1 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2012 VL 105 IS 4 BP 1465 EP 1470 DI 10.1603/EC11405 PG 6 WC Entomology SC Entomology GA 996HG UT WOS:000308074900046 PM 22928330 ER PT J AU Yang, J Punshon, T Guerinot, ML Hirschi, KD AF Yang, Jian Punshon, Tracy Guerinot, Mary Lou Hirschi, Kendal D. TI Plant Calcium Content: Ready to Remodel SO NUTRIENTS LA English DT Review DE calcium; bioavailability; biofortification; bone mineralization; synchrotron X-ray fluorescence (SXRF); oxalate; antinutrient ID MEDICAGO-TRUNCATULA; LOW-PHYTATE; CATION/H+ EXCHANGERS; ARABIDOPSIS SEED; MILK INTOLERANCE; DIETARY CALCIUM; OXALATE CONTENT; FOOD SOURCES; PHYTIC ACID; ABSORPTION AB By identifying the relationship between calcium location in the plant cell and nutrient bioavailability, the plant characteristics leading to maximal calcium absorption by humans can be identified. Knowledge of plant cellular and molecular targets controlling calcium location in plants is emerging. These insights should allow for better strategies for increasing the nutritional content of foods. In particular, the use of preparation-free elemental imaging technologies such as synchrotron X-ray fluorescence (SXRF) microscopy in plant biology may allow researchers to understand the relationship between subcellular location and nutrient bioavailability. These approaches may lead to better strategies for altering the location of calcium within the plant to maximize its absorption from fruits and vegetables. These modified foods could be part of a diet for children and adults identified as at-risk for low calcium intake or absorption with the ultimate goal of decreasing the incidence and severity of inadequate bone mineralization. C1 [Yang, Jian; Hirschi, Kendal D.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Punshon, Tracy; Guerinot, Mary Lou] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Hirschi, Kendal D.] Texas A&M Univ, Vegetable & Fruit Improvement Ctr, College Stn, TX 77845 USA. RP Hirschi, KD (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM jiany@bcm.edu; tracy.punshon@dartmouth.edu; mary.lou.guerinot@dartmouth.edu; kendalh@bcm.edu FU National Institute of Environmental Health Sciences [P42 ES007373-14]; Department of Energy Office of Basic Energy Sciences [DE-FG02-06ER15809]; U.S. Department of Agriculture/Agricultural Research Service [58-62650-6001]; U.S. Department of Agriculture [2005-34402-16401] FX This work was supported by grants from the National Institute of Environmental Health Sciences, Superfund Research Program (grant No. P42 ES007373-14) to TP and MLG and also from the Department of Energy Office of Basic Energy Sciences (grant No. DE-FG02-06ER15809) to MLG, and to KDH by the U.S. Department of Agriculture/Agricultural Research Service (under cooperative agreement 58-62650-6001), and the U.S. Department of Agriculture Grant CSRESS#2005-34402-16401 Designing Foods for Health. We thank the many laboratories who have contributed to the field, both cited and not cited due to space limitations. NR 75 TC 8 Z9 9 U1 0 U2 35 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD AUG PY 2012 VL 4 IS 8 BP 1120 EP 1136 DI 10.3390/nu4081120 PG 17 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 998BG UT WOS:000308211600016 PM 23016135 ER PT J AU Osorio, S Alba, R Nikoloski, Z Kochevenko, A Fernie, AR Giovannoni, JJ AF Osorio, Sonia Alba, Rob Nikoloski, Zoran Kochevenko, Andrej Fernie, Alisdair R. Giovannoni, James J. TI Integrative Comparative Analyses of Transcript and Metabolite Profiles from Pepper and Tomato Ripening and Development Stages Uncovers Species-Specific Patterns of Network Regulatory Behavior SO PLANT PHYSIOLOGY LA English DT Article ID GENE-EXPRESSION ANALYSIS; CAPSICUM-ANNUUM L.; FRUIT-DEVELOPMENT; CAROTENOID BIOSYNTHESIS; HIGHER POLYAMINES; OMICS DATA; ETHYLENE; REVEALS; PLANTS; MICROARRAYS AB Integrative comparative analyses of transcript and metabolite levels from climacteric and nonclimacteric fruits can be employed to unravel the similarities and differences of the underlying regulatory processes. To this end, we conducted combined gas chromatography-mass spectrometry and heterologous microarray hybridization assays in tomato (Solanum lycopersicum; climacteric) and pepper (Capsicum chilense; nonclimacteric) fruits across development and ripening. Computational methods from multivariate and network-based analyses successfully revealed the difference between the covariance structures of the integrated data sets. Moreover, our results suggest that both fruits have similar ethylene-mediated signaling components; however, their regulation is different and may reflect altered ethylene sensitivity or regulators other than ethylene in pepper. Genes involved in ethylene biosynthesis were not induced in pepper fruits. Nevertheless, genes downstream of ethylene perception such as cell wall metabolism genes, carotenoid biosynthesis genes, and the never-ripe receptor were clearly induced in pepper as in tomato fruit. While signaling sensitivity or actual signals may differ between climacteric and nonclimacteric fruit, the evidence described here suggests that activation of a common set of ripening genes influences metabolic traits. Also, a coordinate regulation of transcripts and the accumulation of key organic acids, including malate, citrate, dehydroascorbate, and threonate, in pepper fruit were observed. Therefore, the integrated analysis allows us to uncover additional information for the comprehensive understanding of biological events relevant to metabolic regulation during climacteric and nonclimacteric fruit development. C1 [Osorio, Sonia; Nikoloski, Zoran; Kochevenko, Andrej; Fernie, Alisdair R.] Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany. [Alba, Rob; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr, Ithaca, NY 14853 USA. [Alba, Rob; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Fernie, AR (reprint author), Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany. EM fernie@mpimp-golm.mpg.de RI Osorio, Sonia/M-2258-2014; OI Osorio, Sonia/0000-0002-0159-6091 NR 73 TC 52 Z9 52 U1 1 U2 57 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD AUG PY 2012 VL 159 IS 4 BP 1713 EP 1729 DI 10.1104/pp.112.199711 PG 17 WC Plant Sciences SC Plant Sciences GA 985AX UT WOS:000307236700032 PM 22685169 ER PT J AU Ottis, P Koppe, K Onisko, B Dynin, I Arzberger, T Kretzschmar, H Requena, JR Silva, CJ Huston, JP Korth, C AF Ottis, Philipp Koppe, Katharina Onisko, Bruce Dynin, Irina Arzberger, Thomas Kretzschmar, Hans Requena, Jesus R. Silva, Christopher J. Huston, Joseph P. Korth, Carsten TI Human and rat brain lipofuscin proteome SO PROTEOMICS LA English DT Article DE Aging; ATP synthase; Biomedicine; Fluorescence detection; Mitochondria; Oxidative phosphorylation ID NEURONAL CEROID-LIPOFUSCINOSIS; MITOCHONDRIAL ATP SYNTHASE; RETINAL-PIGMENT EPITHELIUM; TANDEM MASS-SPECTROMETRY; LARGE GENE LISTS; AGE PIGMENT; INFANTILE TYPE; SUBUNIT C; STATISTICAL-MODEL; NERVOUS-SYSTEM AB The accumulation of an autofluorescent pigment called lipofuscin in neurons is an invariable hallmark of brain aging. So far, this material has been considered to be waste material without particular relevance for cellular pathology. However, two lines of evidence argue that lipofuscin may play a yet unidentified role for pathological cellular functions: (i) Genetic forms of premature accumulation of similar autofluorescent material in neuronal ceroid lipofuscinosis indicate a direct disease-associated link to lipofuscin; (ii) Retinal pigment epithelium cell lipofuscin is mechanistically linked to age-associated macular degeneration. Here, we purified autofluorescent material from the temporal and hippocampal cortices of three different human individuals by a two-step ultracentrifugation on sucrose gradients. For human brain lipofuscin, we could identify a common set of 49 (among > 200 total) proteins that are mainly derived from mitochondria, cytoskeleton, and cell membrane. This brain lipofuscin proteome was validated in an interspecies comparison with whole brain rat lipofuscin (total > 300 proteins), purified by the same procedure, yielding an overlap of 32 proteins (64%) between lipofuscins of both species. Our study is the first to characterize human and rat brain lipofuscin and identifies high homology, pointing to common cellular pathomechanisms of age-associated lipofuscin accumulation despite the huge (40-fold) difference in the lifespan of these species. Our identification of these distinct proteins will now allow research in disturbed molecular pathways during age-associated dysfunctional lysosomal degradation. C1 [Korth, Carsten] Univ Dusseldorf, Sch Med, Dept Neuropathol, Neurodegenerat Unit, D-40225 Dusseldorf, Germany. [Onisko, Bruce] OniPro, Kensington, CA USA. [Onisko, Bruce; Dynin, Irina; Silva, Christopher J.] USDA, Albany, CA USA. [Arzberger, Thomas; Kretzschmar, Hans] Univ Munich, Dept Neuropathol, Munich, Germany. [Requena, Jesus R.] Univ Santiago de Compostela IDIS, Dept Med, Santiago, Spain. [Requena, Jesus R.] Univ Santiago de Compostela IDIS, CIMUS Biomed Res Inst, Santiago, Spain. [Huston, Joseph P.] Univ Dusseldorf, Dept Expt Psychol, Ctr Behav Neurosci, D-40225 Dusseldorf, Germany. RP Korth, C (reprint author), Univ Dusseldorf, Sch Med, Dept Neuropathol, Neurodegenerat Unit, Moorenstr 5, D-40225 Dusseldorf, Germany. EM ckorth@uni-duesseldorf.de OI Onisko, Bruce/0000-0003-4657-8257; Silva, Christopher/0000-0003-4521-6377 FU EU-FP7 PRIORITY; DFG [GRK1033]; KNDD-Demtest; [Hu 306/27-2] FX This research has been funded by grant from the EU-FP7 PRIORITY to C. K. and J.R.R., and grants form the DFG (GRK1033) and KNDD-Demtest to C. K., and Hu 306/27-2 to J.P.H. NR 54 TC 18 Z9 19 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1615-9853 J9 PROTEOMICS JI Proteomics PD AUG PY 2012 VL 12 IS 15-16 SI SI BP 2445 EP 2454 DI 10.1002/pmic.201100668 PG 10 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 996NZ UT WOS:000308098200006 PM 22707227 ER PT J AU Bus, VGM van de Weg, WE Peil, A Dunemann, F Zini, E Laurens, FND Blazek, J Hanke, V Forsline, PL AF Bus, Vincent G. M. van de Weg, W. Eric Peil, Andreas Dunemann, Frank Zini, Elena Laurens, Francois N. D. Blazek, Jan Hanke, Viola Forsline, Philip L. TI The role of Schmidt 'Antonovka' in apple scab resistance breeding SO TREE GENETICS & GENOMES LA English DT Review DE Apple scab; Venturia inaequalis; Malus x domestica; Major gene; Quantitative resistance; Antonovka ID VENTURIA-INAEQUALIS; DRESDEN-PILLNITZ; MOLECULAR CHARACTERIZATION; CANDIDATE GENES; BROAD-SPECTRUM; RAPD MARKERS; RACE 6; MALUS; CULTIVARS; PATHOGEN AB 'Antonovka' has long been recognised as a major source of scab (Venturia inaequalis) resistance useful for apple breeding worldwide. Both major gene resistances in the form of the Rvi10 and Rvi17 and quantitative resistance, collectively identified as VA, have been identified in different accessions of 'Antonovka'. Most of the 'Antonovka' scab resistance used in apple-breeding programmes around the world can be traced back to Schmidt 'Antonovka' and predominantly its B VIII progenies 33,25 (PI 172623), 34,6 (PI 172633), 33,8 (PI 172612) and 34,5 (PI 172632). Using genetic profile reconstruction, we have identified "common 'Antonovka' " as the progenitor of the B VIII family, which is consistent with it having been a commercial cultivar in Poland and the single source of scab resistance used by Dr. Martin Schmidt. The major 'Antonovka' scab resistance genes mapped to date are located either very close to Rvi6, or about 20-25 cM above it, but their identities need further elucidation. The presence of the 139 bp allele of the CH-Vf1 microsatellite marker known to be associated with Rvi17 (Va1) in most of the 'Antonovka' germplasm used in breeding suggests that it plays a central role in the resistance. The nature and the genetic relationships of the scab resistance in these accessions as well as a number of apple cultivars derived from 'Antonovka', such as, 'Freedom', 'Burgundy' and 'Angold', are discussed. The parentage of 'Reglindis' is unclear, but the cultivar commercialised as 'Reglindis' was confirmed to be an Rvi6 cultivar. C1 [Bus, Vincent G. M.] New Zealand Inst Plant & Food Res, Hawkes Bay Res Ctr, Havelock N 4157, New Zealand. [van de Weg, W. Eric] Wageningen UR Plant Breeding, NL-6700 AA Wageningen, Netherlands. [Peil, Andreas; Hanke, Viola] Julius Kuhn Inst, Inst Breeding Res Hort & Fruit Crops, D-01326 Dresden, Germany. [Dunemann, Frank] Julius Kuhn Inst, Inst Breeding Res Hort & Fruit Crops, D-06484 Quedlinburg, Germany. [Zini, Elena] Edmund Mach Fdn, Res & Innovat Ctr, Agr Inst San Michele All Adige IASMA, I-38010 San Michele All Adige Tr, Italy. [Zini, Elena] Univ Bologna, Dept Fruit Tree & Woody Plant Sci, I-40121 Bologna, Italy. [Laurens, Francois N. D.] GenHort, Inst Natl Rech Agron, F-49071 Beaucouze, France. [Blazek, Jan] Res & Breeding Inst Pomol, Holovousy 50801, Horice, Czech Republic. [Forsline, Philip L.] Cornell Univ, ARS, USDA, Plant Genet Resources Unit, Geneva, NY 14456 USA. RP Bus, VGM (reprint author), New Zealand Inst Plant & Food Res, Hawkes Bay Res Ctr, Private Bag 1401, Havelock N 4157, New Zealand. EM vincent.bus@plantandfood.co.nz RI Van de Weg, Eric/G-3225-2011; Bus, Vincent/B-1318-2011; Hanke, Magda-Viola/P-5445-2014 OI Van de Weg, Eric/0000-0002-9443-5974; Bus, Vincent/0000-0003-4071-4457; Hanke, Magda-Viola/0000-0002-6788-4934 NR 101 TC 4 Z9 5 U1 3 U2 43 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD AUG PY 2012 VL 8 IS 4 BP 627 EP 642 DI 10.1007/s11295-012-0470-2 PG 16 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 985TA UT WOS:000307291800001 ER PT J AU Stewart, JF Tauer, CG Nelson, CD AF Stewart, John F. Tauer, Charles G. Nelson, C. D. TI Bidirectional introgression between loblolly pine (Pinus taeda L.) and shortleaf pine (P-echinata Mill.) has increased since the 1950s SO TREE GENETICS & GENOMES LA English DT Article DE Ecological genetics; Hybridization; Introgression ID MULTILOCUS GENOTYPE DATA; POPULATIONS; HYBRIDIZATION; POLLEN; EXTINCTION; DISPERSAL; INFERENCE; MOVEMENT; OAK AB Hybridization between loblolly pine and shortleaf pine has been shown in sympatric and allopatric portions of the species native ranges. In this study, we used 25 microsatellite markers to determine the hybrid status of 165 and 151 loblolly pine and shortleaf pine trees sampled across their ranges, respectively, and to estimate population differentiation within these species. Estimated differentiation (I broken vertical bar (PT)) for these current-day samples was significantly higher in both species-0.115 in loblolly pine and 0.146 in shortleaf pine-than for trees planted from seed collected from the same locations in the 1950s. These increases are likely due to anthropogenic causes such as habitat fragmentation. In addition, the proportion of hybrids rose dramatically in both species: 27.3% hybrids in loblolly pine populations and 46.7% hybrids in shortleaf pine populations compared to rates of 4.5% and 3.3%, respectively, in the 1950s populations. Our results suggest that shortleaf pine and remnant naturally regenerated loblolly pine are at risk. C1 [Stewart, John F.; Tauer, Charles G.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Nelson, C. D.] US Forest Serv, USDA, So Inst Forest Genet, Saucier, MS USA. RP Stewart, JF (reprint author), Oklahoma State Univ, Stillwater, OK 74078 USA. EM john.stewart@okstate.edu FU Southern Research Station [SRS 05-CA-11330126-168]; Oklahoma State University Agricultural Experiment Station FX We would like to thank all of the foresters who helped us gather the needles for this study: Scott Schlarbaum and David Griffin of the University of Tennessee; Roy Ward of the Ames Plantation; Thomas Hall of Pennsylvania State University; Russ Pohl of the Georgia Forestry Commission; Scott Merkle of the University of Georgia; William E. Dienst of the U. S. Forest Service in North Carolina; Gregory Powell and Dale Rye of the University of Florida; Larry Miller and I.N. Brown of Texas A&M University; Michael Mills of Claybourn Waters Company; Brad Claus of the Louisiana Department of Agriculture and Forestry; Randall Rousseau of Mississippi State University; David Gwaze of the Missouri Department of Conservation; and Robert Heinemann of the Kiamichi Forestry Research Station. We would also like to acknowledge Mary Tsien, wife of author John Stewart, for creating the map figure in this study. Funding partly originated from the Southern Research Station (Cooperative Agreement No. SRS 05-CA-11330126-168). This study was supported by the Oklahoma State University Agricultural Experiment Station. NR 39 TC 8 Z9 8 U1 0 U2 24 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD AUG PY 2012 VL 8 IS 4 BP 725 EP 735 DI 10.1007/s11295-011-0459-2 PG 11 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 985TA UT WOS:000307291800009 ER PT J AU Hadziabdic, D Wang, XW Wadl, PA Rinehart, TA Ownley, BH Trigiano, RN AF Hadziabdic, Denita Wang, Xinwang Wadl, Phillip A. Rinehart, Timothy A. Ownley, Bonnie H. Trigiano, Robert N. TI Genetic diversity of flowering dogwood in the Great Smoky Mountains National Park SO TREE GENETICS & GENOMES LA English DT Article DE Cornus florida; Dogwood anthracnose; Gene flow; Microsatellites; Mortality; Native dogwood populations; Genetic diversity ID CORNUS-FLORIDA L.; DISCULA-DESTRUCTIVA REDLIN; POPULATION-STRUCTURE; MICROSATELLITE ALLELES; FOREST COMPOSITION; MITOCHONDRIAL-DNA; COMPUTER-PROGRAM; SEED DISPERSAL; NORTH-AMERICA; ANTHRACNOSE AB In the past three decades, flowering dogwood (Cornus florida) populations have experienced severe declines caused by dogwood anthracnose. Mortality has ranged from 48% to 98%, raising the concern that the genetic diversity of this native tree has been reduced significantly. In this study, we investigated levels of genetic diversity and population structure of flowering dogwood populations in the Great Smoky Mountains National Park (GSMNP). Understanding the factors influencing geographic distribution of genetic variation is one of the major concerns for preserving biodiversity and conservation of native populations. Eighteen microsatellite loci were used to evaluate the level and distribution of genetic variation of native flowering dogwood trees throughout the GSMNP. Significant genetic structure exists at both landscape and local levels. Two genetic clusters exist within the park and are separated by the main dividing ridges of the Great Smoky Mountains. The differentiation of the clusters is subtle, but statistically significant. Gene flow, evident through low-elevation corridors, indicates that nonrandom mating occurs between related individuals despite wide dispersal of seeds. Although high mortality rate and reduced fecundity caused by dogwood anthracnose severely affected native flowering dogwood populations throughout the entire GSMNP, this study confirmed that considerable genetic diversity still exists at the population level. It seems unlikely that recent demographic dynamics have resulted in a depletion of genetic variation. C1 [Hadziabdic, Denita; Wadl, Phillip A.; Ownley, Bonnie H.; Trigiano, Robert N.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Wang, Xinwang] Texas A&M Syst, Texas AgriLife Res & Extens Ctr, Dallas, TX 75252 USA. [Rinehart, Timothy A.] ARS, USDA, So Hort Lab, Poplarville, MS 39470 USA. RP Hadziabdic, D (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 2431 Joe Johnson Dr, Knoxville, TN 37996 USA. EM dhadziab@utk.edu FU United States Department of Agriculture [58-6404-2-0057] FX The authors thank the United States Department of Agriculture (agreement number 58-6404-2-0057) for financial support. NR 76 TC 8 Z9 8 U1 3 U2 21 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD AUG PY 2012 VL 8 IS 4 BP 855 EP 871 DI 10.1007/s11295-012-0471-1 PG 17 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 985TA UT WOS:000307291800019 ER PT J AU Wegrzyn, JL Main, D Figueroa, B Choi, M Yu, J Neale, DB Jung, S Lee, T Staton, M Zheng, P Ficklin, S Cho, I Peace, C Evans, K Volk, G Oraguzie, N Chen, C Olmstead, M Gmitter, G Abbott, AG AF Wegrzyn, J. L. Main, D. Figueroa, B. Choi, M. Yu, J. Neale, D. B. Jung, S. Lee, T. Staton, M. Zheng, P. Ficklin, S. Cho, I. Peace, C. Evans, K. Volk, G. Oraguzie, N. Chen, C. Olmstead, M. Gmitter, G., Jr. Abbott, A. G. TI Uniform standards for genome databases in forest and fruit trees (vol 5, pg 549, 2012) SO TREE GENETICS & GENOMES LA English DT Correction C1 [Wegrzyn, J. L.; Figueroa, B.; Choi, M.; Yu, J.; Neale, D. B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Main, D.; Jung, S.; Lee, T.; Zheng, P.; Ficklin, S.; Peace, C.; Evans, K.; Oraguzie, N.] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Cho, I.] Saginaw Valley State Univ, Dept Comp Sci, Saginaw, MI 48710 USA. [Staton, M.] Clemson Univ, Genom Inst, Clemson, SC 29634 USA. [Volk, G.] ARS, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. [Olmstead, M.] Univ Florida, Hort Sci Dept IFAS, Gainesville, FL 32611 USA. [Chen, C.; Gmitter, G., Jr.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Abbott, A. G.] Clemson Univ, Dept Biochem & Genet, Clemson, SC 29634 USA. RP Wegrzyn, JL (reprint author), Univ Calif Davis, Dept Plant Sci, Mail Stop 4, Davis, CA 95616 USA. EM jlwegrzyn@ucdavis.edu; dorrie@wsu.edu OI Wegrzyn, Jill/0000-0001-5923-0888 NR 1 TC 0 Z9 0 U1 0 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD AUG PY 2012 VL 8 IS 4 BP 941 EP 941 DI 10.1007/s11295-012-0541-4 PG 1 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 985TA UT WOS:000307291800026 ER PT J AU Long, TM Su, YK Headman, J Higbee, A Willis, LB Jeffries, TW AF Long, Tanya M. Su, Yi-Kai Headman, Jennifer Higbee, Alan Willis, Laura B. Jeffries, Thomas W. TI Cofermentation of Glucose, Xylose, and Cellobiose by the Beetle-Associated Yeast Spathaspora passalidarum SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RECOMBINANT SACCHAROMYCES-CEREVISIAE; INCREASES ETHANOL-PRODUCTION; STIPITIS NRRL Y-7124; PICHIA-STIPITIS; CANDIDA-INTERMEDIA; SP NOV.; FERMENTATION; REDUCTASE; EXPRESSION; CHROMATOGRAPHY AB Fermentation of cellulosic and hemicellulosic sugars from biomass could resolve food-versus-fuel conflicts inherent in the bioconversion of grains. However, the inability to coferment glucose and xylose is a major challenge to the economical use of lignocellulose as a feedstock. Simultaneous cofermentation of glucose, xylose, and cellobiose is problematic for most microbes because glucose represses utilization of the other saccharides. Surprisingly, the ascomycetous, beetle-associated yeast Spathaspora passalidarum, which ferments xylose and cellobiose natively, can also coferment these two sugars in the presence of 30 g/liter glucose. S. passalidarum simultaneously assimilates glucose and xylose aerobically, it simultaneously coferments glucose, cellobiose, and xylose with an ethanol yield of 0.42 g/g, and it has a specific ethanol production rate on xylose more than 3 times that of the corresponding rate on glucose. Moreover, an adapted strain of S. passalidarum produced 39 g/liter ethanol with a yield of 0.37 g/g sugars from a hardwood hydrolysate. Metabolome analysis of S. passalidarum before onset and during the fermentations of glucose and xylose showed that the flux of glycolytic intermediates is significantly higher on xylose than on glucose. The high affinity of its xylose reductase activities for NADH and xylose combined with allosteric activation of glycolysis probably accounts in part for its unusual capacities. These features make S. passalidarum very attractive for studying regulatory mechanisms enabling bioconversion of lignocellulosic materials by yeasts. C1 [Long, Tanya M.; Su, Yi-Kai; Headman, Jennifer; Higbee, Alan; Willis, Laura B.; Jeffries, Thomas W.] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Su, Yi-Kai] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI USA. [Headman, Jennifer; Willis, Laura B.; Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Willis, Laura B.; Jeffries, Thomas W.] USDA, Forest Prod Lab, Madison, WI 53705 USA. RP Jeffries, TW (reprint author), Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. EM twjeffri@wisc.edu RI Jeffries, Thomas/I-8576-2012 OI Jeffries, Thomas/0000-0001-7408-4065 FU DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494]; Heart of Wisconsin (Wisconsin Rapids); USDA, Forest Products Laboratory FX This work was funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494) and by a grant from the Heart of Wisconsin (Wisconsin Rapids) to the University of Wisconsin, Madison. T.W.J. is supported by the USDA, Forest Products Laboratory. NR 47 TC 28 Z9 29 U1 3 U2 39 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2012 VL 78 IS 16 BP 5492 EP 5500 DI 10.1128/AEM.00374-12 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 983SR UT WOS:000307139500003 PM 22636012 ER PT J AU Timper, P Davis, R Jagdale, G Herbert, J AF Timper, Patricia Davis, Richard Jagdale, Ganpati Herbert, John TI Resiliency of a nematode community and suppressive service to tillage and nematicide application SO APPLIED SOIL ECOLOGY LA English DT Article DE Nematode community; Nematicides; Plant-feeding nematodes; Predators; Suppressive soil; Tillage ID PLANT-PARASITIC NEMATODES; SOIL FOOD-WEB; PASTEURIA-PENETRANS; BIOLOGICAL-CONTROL; MATURITY INDEX; MELOIDOGYNE-ARENARIA; PREDATORY NEMATODE; METHYL-BROMIDE; SOYBEAN FIELD; AGROECOSYSTEMS AB We hypothesized that populations of predatory and omnivorous nematodes would be slower to recover from conventional tillage and nematicide application than other nematode trophic groups, and that lower populations of predators and omnivores would lead to greater survival and reproduction of plant-feeding nematodes (i.e., pest resurgence). A field study was conducted from 2008 to 2010 with two tillage regimes (strip and conventional) and two nematicide treatments (1,3-dichloropropene + aldicarb and a no-nematicide control) with six replications arranged in a split-plot design. Soil samples were collected pre-fumigation/tillage, post plant, and mid season during each year. Nematodes were extracted from soil and identified to genus. The field site had relatively high populations of predatory and omnivorous nematodes and abundance of these feeding groups translated into a relatively high Structure Index (>= 60) in the control plots. Tillage had little impact on the nematode community, but the nematicides reduced numbers of all trophic groups compared to the control at post plant and mid season. However, for bacterial and plant feeders, there was also a consistent lingering effect of the nematicides the following year at pre-fumigation. Omnivores and predators were not severely impacted by the nematicide treatment; populations of both groups repeatedly recovered by the following spring from the yearly application of nematicides, with the exception of predators in 2008. We used two bioassays to measure the suppressive service of the soil community: one determined survival of the reniform nematode (Rotylenchulus reniformis) and the other reproduction of Meloidogyne arenaria on peanut. Survival of R. remforrnis was greater in defaunated compared to native soil indicating that the soil contained organisms that consumed nematodes. Application of nematicides led to an increase in survival of the It remformis from 53% in the control to 78% in treated plots at post plant, and from 55% in the control plots to 66% in treated plots at mid season. Likewise, reproduction of M. arenaria was greater in soil treated with nematicides. At post plant, survival (%) of R. reniformis was negatively correlated with abundance of predators + omnivores. Organisms other than nematodes were likely involved in the suppressive service. This was particularly evident at pre-fumigation/tillage, when survival of R. reniformis was lower in plots previously treated with nematicides than in control plots. The nematicides may have altered the soil community to allow a fungal, bacterial, or invertebrate antagonist of nematodes to increase in abundance. Published by Elsevier B.V. C1 [Timper, Patricia; Davis, Richard] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Jagdale, Ganpati] Univ Georgia, Extens Nematol Lab, Dept Plant Pathol, Athens, GA 30605 USA. [Herbert, John] Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA. RP Timper, P (reprint author), USDA ARS, Crop Protect & Management Res Unit, POB 748, Tifton, GA 31793 USA. EM Patricia.Timper@ars.usda.gov NR 59 TC 11 Z9 11 U1 1 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD AUG PY 2012 VL 59 BP 48 EP 59 DI 10.1016/j.apsoil.2012.04.001 PG 12 WC Soil Science SC Agriculture GA 991FM UT WOS:000307686900007 ER PT J AU Gomez-Hernandez, M Williams-Linera, G Guevara, R Lodge, DJ AF Gomez-Hernandez, Marko Williams-Linera, Guadalupe Guevara, Roger Lodge, D. Jean TI Patterns of macromycete community assemblage along an elevation gradient: options for fungal gradient and metacommunity analyse SO BIODIVERSITY AND CONSERVATION LA English DT Article DE Altitudinal gradient; Community turnover; Distribution; Ectomycorrhizal; Environment variation; Macrofungal diversity; Methods; Xylophagous ID EUCALYPTUS-OBLIQUA FOREST; HIGHER-TAXON APPROACH; DECAYING BEECH LOGS; SPECIES RICHNESS; MACROFUNGAL ASSEMBLAGES; ECTOMYCORRHIZAL FUNGI; SOUTHERN TASMANIA; BETA-DIVERSITY; FRUIT BODIES; ABUNDANCE AB Gradient analysis is rarely used in studies of fungal communities. Data on macromycetes from eight sites along an elevation gradient in central Veracruz, Mexico, were used to demonstrate methods for gradient analysis that can be applied to studies of communities of fungi. Selected sites from 100 to 3,500 m altitude represent tropical dry forest, tropical montane cloud forest, conifer forest, and their ecotones. From May to October 2010, macromycetes were collected monthly within ten 10 x 10 m permanent plots per site. In total, 672 individuals of 213 species of macromycetes were recorded. Models for richness and diversity for all macromycete and ectomycorrhizal communities displayed peaks in the mid-part of the gradient, and a tendency to increase with elevation, whereas xylophagous fungi displayed a peak in the mid-lower part but tended to decrease with elevation. Cluster and Maximum Likelihood analyses distinguished four communities for both macromycetes and trees, but plant and fungal communities were only partly concordant. Canonical correspondence analysis indicated that macromycete distribution along the gradient is related to slope, relative humidity, soil temperature, soil water content, canopy openness, and litter depth. Spearman's correlation and regression trees suggested that air and soil temperature, relative humidity, soil water content, canopy openness, vegetation structure and tree species richness were most strongly related to macrofungal functional groups, but these environmental variables were often correlated to the forest type and may not be causal. Variation in the environment along the elevation gradient differentially affected macromycete functional groups. Results from the different methods used in this work were concordant and showed significant patterns. C1 [Lodge, D. Jean] US Forest Serv, USDA, No Res Stn, Luquillo, PR 00773 USA. [Gomez-Hernandez, Marko; Williams-Linera, Guadalupe; Guevara, Roger] Inst Ecol, Xalapa 91070, Veracruz, Mexico. RP Lodge, DJ (reprint author), US Forest Serv, USDA, No Res Stn, Luquillo, PR 00773 USA. EM djlodge@caribe.net RI Williams-Linera, Guadalupe/C-1242-2014; Guevara, Roger/F-3287-2010 OI Williams-Linera, Guadalupe/0000-0001-6341-6570; Guevara, Roger/0000-0003-0768-3580 FU Luquillo LTER (NSF) [DEB-0218039]; IITF, USDA Forest Service; Consejo Nacional de Ciencia y Tecnologia FX The corresponding author thanks the Luquillo LTER for support (NSF grant DEB-0218039) to the University of Puerto Rico and IITF, USDA Forest Service. The authors especially thank two Luquillo LTER scientists, Dr M. Willig of University of Connecticut for initial advice, reprints and preprints on metacommunity analysis, and J. K. Zimmerman for additional advice. We thank E. Gandara and M. Cruz for their assistance in the field and with identifications. This research was partially supported by a scholarship to the first author from Consejo Nacional de Ciencia y Tecnologia. NR 57 TC 16 Z9 16 U1 3 U2 56 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3115 J9 BIODIVERS CONSERV JI Biodivers. Conserv. PD AUG PY 2012 VL 21 IS 9 SI SI BP 2247 EP 2268 DI 10.1007/s10531-011-0180-3 PG 22 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 985UG UT WOS:000307295400005 ER PT J AU Page, WG Jenkins, MJ Runyon, JB AF Page, Wesley G. Jenkins, Michael J. Runyon, Justin B. TI Mountain pine beetle attack alters the chemistry and flammability of lodgepole pine foliage SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID WESTERN NORTH-AMERICA; FIRE BEHAVIOR; CROWN FIRE; PLANT FLAMMABILITY; BARK BEETLES; FOREST FUELS; HEAT-CONTENT; MOISTURE; IGNITION; EXTRACTIVES AB During periods with epidemic mountain pine beetle (Dendroctonus ponderosae Hopkins) populations in lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) forests, large amounts of tree foliage are thought to undergo changes in moisture content and chemistry brought about by tree decline and death. However, many of the presumed changes have yet to be quantified. In this study, we quantified and compared fuel moisture, chemistry, and resulting flammability of bark beetle affected foliage in terms of ignitability, combustibility, consumability, and sustainability at a site in far eastern Idaho, USA. Results revealed substantial decreases in moisture content, the proportion of starches and sugars, and crude fat and increases in the proportions of lignin, cellulose, and hemicellulose in foliage of trees attacked in the previous year (yellow foliage) or more than two years previously (red foliage). Increases in emission rates of several terpenes that were correlated with flammability were also detected in yellow foliage. The flammability of fresh yellow and red foliage increased with regard to ignitability and sustainability, with shorter times to ignition, lower temperatures at ignition, and higher heat yields when compared with unattacked green foliage. Our results confirm the overwhelming importance of fuel moisture on flammability and suggest that fuel chemical composition also has significant effects on lodgepole pine foliage flammability. C1 [Page, Wesley G.; Jenkins, Michael J.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Runyon, Justin B.] US Forest Serv, Rocky Mt Res Stn, USDA, Bozeman, MT 59717 USA. RP Jenkins, MJ (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM mike.jenkins@usu.edu OI Runyon, Justin B./0000-0002-0271-0511 FU Joint Fire Science Program Project [11-1-4-16]; Utah Agricultural Experiment Station, Utah State University FX Funding for this project was provided by Joint Fire Science Program Project 11-1-4-16. The comments of M. E. Alexander, B.J. Bentz, E. W. Evans, E. G. Hebertson, M. R. Kuhns, and two anonymous reviewers were appreciated. The authors thank C. Stam for help with fieldwork, W. Lindquist for assistance with graphics, and L. Teng at Utah State University's BioEnergy Center for lab equipment support. This research was supported by the Utah Agricultural Experiment Station, Utah State University, and approved as journal paper number 8408. NR 66 TC 24 Z9 24 U1 6 U2 60 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD AUG PY 2012 VL 42 IS 8 BP 1631 EP 1647 DI 10.1139/X2012-094 PG 17 WC Forestry SC Forestry GA 983KT UT WOS:000307118900020 ER PT J AU Veverica, TJ Kane, ES Kasischke, ES AF Veverica, Timothy J. Kane, Evan S. Kasischke, Eric S. TI Tamarack and black spruce adventitious root patterns are similar in their ability to estimate organic layer depths in northern temperate forests SO CANADIAN JOURNAL OF SOIL SCIENCE LA English DT Article DE Adventitious roots; fire; peat; black spruce; tamarack; larch; organic soil ID FIRE SEVERITY; BOREAL FOREST; CARBON; ALASKA AB Veverica, T. J., Kane, E. S. and Kasischke, E. S. 2012. Tamarack and black spruce adventitious root patterns are similar in their ability to estimate organic layer depths in northern temperate forests. Can. J. Soil Sci. 92: 799-802. Organic layer consumption during forest fires is hard to quantify. These data suggest that the adventitious root methods developed for reconstructing organic layer depths following wildfires in boreal black spruce forests can also be applied to mixed tamarack forests growing in temperate regions with glacially transported soils. C1 [Kane, Evan S.] US Forest Serv, No Res Stn, Houghton, MI USA. [Veverica, Timothy J.] Michigan Technol Univ, Dept Biol, Houghton, MI USA. [Kasischke, Eric S.] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. RP Kane, ES (reprint author), US Forest Serv, No Res Stn, Houghton, MI USA. EM eskane@mtu.edu FU Michigan Tech. University Summer Undergraduate Research Fellowship (SURF); MTU Honors Institute FX Support for T.J.V. came from the Michigan Tech. University Summer Undergraduate Research Fellowship (SURF) and the MTU Honors Institute. We thank Heather Salmon for field assistance and Isla Myers-Smith and Oliver Gailing for aid in translation to French. Dr. Paul Sanborn and two anonymous reviewers provided helpful comments which improved this manuscript. NR 10 TC 2 Z9 2 U1 0 U2 19 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4271 EI 1918-1841 J9 CAN J SOIL SCI JI Can. J. Soil Sci. PD AUG PY 2012 VL 92 IS 5 BP 799 EP 802 DI 10.4141/CJSS2011-111 PG 4 WC Soil Science SC Agriculture GA 994IQ UT WOS:000307924500009 ER PT J AU Scott, JM Mathiasen, RL AF Scott, Jared M. Mathiasen, Robert L. TI Assessing Growth and Mortality of Bristlecone Pine Infected by Dwarf Mistletoe Using Dendrochronology SO FOREST SCIENCE LA English DT Article DE Arceuthobium microcarpum; northern Arizona.; Pinus aristata; tree-ring analysis ID NORTHERN ARIZONA; PONDEROSA PINE; DOUGLAS-FIR; SPRUCE; MOUNTAINS; USA; TEMPERATURE; LANDSCAPE AB Bristlecone pine dwarf mistletoe (Arceuthobium microcarpum [Engelm.] Hawksw. & Wiens subsp. aristatae J.M.Scott & Mathiasen) severely parasitizes Rocky Mountain bristlecone pine (Pinus aristata Engelm.) on the San Francisco Peaks, Arizona. We estimated the current incidence of infection and extent of mortality in a mistletoe-infested stand of bristlecone pine on Schultz Peak, a southeast subsidiary ridge of the San Francisco Peaks. Dendrochronological techniques were used to compare radial growth between lightly, moderately, and severely infected and noninfected bristlecone pines. We also paired limber pine (Pinus flexilis James) with noninfected and infected bristlecone pines as another method of estimating the effects of the mistletoe on radial growth. Results suggested that the incidence of infection has increased by approximately 10%, and mortality has increased by approximately 20% since the mid-1970s. Severely infected bristlecone pines had significant growth losses and less annual variation in growth than limber pine or lightly infected and noninfected bristlecone pines. Spatial and temporal trends in mortality of bristlecone pines were measured in two large mortality locations on Schultz Peak. Cross-dating cores from dead bristlecone pines indicated that mortality had rapidly increased starting in 1996. Years of high mortality were related to both high mountain pine beetle (Dendroctonus ponderosae Hopkins) activity and drought conditions. FOR. Sci. 58(4):366-376. C1 [Scott, Jared M.] US Forest Serv, Williams, AZ 86046 USA. [Mathiasen, Robert L.] No Arizona Univ, Flagstaff, AZ 86011 USA. RP Scott, JM (reprint author), US Forest Serv, 800 S 6th St, Williams, AZ 86046 USA. EM jaredmscott@fs.fed.us; robert.mathiasen@nau.edu FU NAU Mission Research Program; Laboratory of Tree-Ring Research at the University of Arizona, Tucson FX We thank Dr. Pete Fule for his assistance with tree-ring analyses. We also thank the Dendroecology Laboratory at Northern Arizona University (NAU) for providing equipment and assistance with tree-ring analyses. J. White, I. Johnson, and L. Smith assisted with data collection and analyses. The NAU statistical consulting laboratory also provided help with data analyses. We would also like to thank the two anonymous reviewers whose comments were helpful in allowing this study to be acceptable for publication. The study was conducted in cooperation with the US Forest Service and was primarily funded by the NAU Mission Research Program with additional funding from the Laboratory of Tree-Ring Research at the University of Arizona, Tucson. NR 35 TC 1 Z9 1 U1 4 U2 28 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD AUG PY 2012 VL 58 IS 4 BP 366 EP 376 DI 10.5849/forsci.10-142 PG 11 WC Forestry SC Forestry GA 994FI UT WOS:000307915900005 ER PT J AU Duan, JL Wu, JJ Liu, Y Xiao, JH Zhao, GY Gu, YQ Jia, JZ Kong, XY AF Duan, Jialei Wu, Jiajie Liu, Yue Xiao, Jianhui Zhao, Guangyao Gu, Yongqiang Jia, Jizeng Kong, Xiuying TI New cis-regulatory elements in the Rht-D1b locus region of wheat SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Wheat; Plant height reduced gene; Rht-D1b; Microcolinearity; Comparative genomics; CNS ID CONSERVED NONCODING SEQUENCES; MAXIMUM-LIKELIHOOD; SIGNALING PATHWAY; BINDING SITES; PLANT; EVOLUTION; DATABASE; PROTEIN; GENOME; GENES AB Fifteen gene-containing BACs with accumulated length of 1.82-Mb from the Rht-D1b locus region were sequenced and compared in detail with the orthologous regions of rice, sorghum, and maize. Our results show that Rht-D1b represents a conserved genomic region as implied by high gene sequence identity, good maintenance of gene colinearity, and the presence of multiple conserved noncoding sequences (CNSs) that are shared by other grass species. Eight cis-regulatory elements in these CNSs around grass DELLA genes were detected. C1 [Duan, Jialei; Wu, Jiajie; Liu, Yue; Xiao, Jianhui; Zhao, Guangyao; Jia, Jizeng; Kong, Xiuying] Chinese Acad Agr Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Natl Key Facil Crop Gene Resources & Genet Improv, Minist Agr,Inst Crop Sci, Beijing 100081, Peoples R China. [Gu, Yongqiang] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Duan, Jialei] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China. RP Jia, JZ (reprint author), Chinese Acad Agr Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Natl Key Facil Crop Gene Resources & Genet Improv, Minist Agr,Inst Crop Sci, 12 South St, Beijing 100081, Peoples R China. EM duanjl@gmail.com; jzjia@mail.caas.net.cn; xiuyingkong@yahoo.com FU National Transgenic Research Project [2009ZX08009-110B]; National Basic Research Program of China [2010CB125902] FX We would like to thank Lei Pan and Lingli Zheng for their assistance on BAC sequencing and Lifang Zhang and Yuhong Liu for mapping support. The work was supported by grants from National Transgenic Research Project (2009ZX08009-110B) and National Basic Research Program of China (2010CB125902). NR 50 TC 9 Z9 9 U1 1 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD AUG PY 2012 VL 12 IS 3 BP 489 EP 500 DI 10.1007/s10142-012-0283-2 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA 998KY UT WOS:000308238900007 PM 22592657 ER PT J AU Anderson, JV Dogramaci, M Horvath, DP Foley, ME Chao, WS Suttle, JC Thimmapuram, J Hernandez, AG Ali, S Mikel, MA AF Anderson, James V. Dogramaci, Muenevver Horvath, David P. Foley, Michael E. Chao, Wun S. Suttle, Jeffrey C. Thimmapuram, Jyothi Hernandez, Alvaro G. Ali, Shahjahan Mikel, Mark A. TI Auxin and ABA act as central regulators of developmental networks associated with paradormancy in Canada thistle (Cirsium arvense) SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Canada thistle; Dormancy; ESTs; Hormone profiling ID SPURGE EUPHORBIA-ESULA; ABSCISIC-ACID; LEAFY SPURGE; GENE-EXPRESSION; TRANSCRIPTION FACTOR; DORMANCY STATUS; CROWN BUDS; GIBBERELLIN BIOSYNTHESIS; HELIANTHUS-ANNUUS; WEED MANAGEMENT AB Dormancy in underground vegetative buds of Canada thistle, an herbaceous perennial weed, allows escape from current control methods and contributes to its invasive nature. In this study, similar to 65 % of root sections obtained from greenhouse propagated Canada thistle produced new vegetative shoots by 14 days post-sectioning. RNA samples obtained from sectioned roots incubated 0, 24, 48, and 72 h at 25A degrees C under 16:8 h light-dark conditions were used to construct four MID-tagged cDNA libraries. Analysis of in silico data obtained using Roche 454 GS-FLX pyrosequencing technologies identified molecular networks associated with paradormancy release in underground vegetative buds of Canada thistle. Sequencing of two replicate plates produced similar to 2.5 million ESTs with an average read length of 362 bases. These ESTs assembled into 67358 unique sequences (21777 contigs and 45581 singlets) and annotation against the Arabidopsis database identified 15232 unigenes. Among the 15232 unigenes, we identified processes enriched with transcripts involved in plant hormone signaling networks. To follow-up on these results, we examined hormone profiles in roots, which identified changes in abscisic acid (ABA) and ABA metabolites, auxins, and cytokinins post-sectioning. Transcriptome and hormone profiling data suggest that interaction between auxin- and ABA-signaling regulate paradormancy maintenance and release in underground adventitious buds of Canada thistle. Our proposed model shows that sectioning-induced changes in polar auxin transport alters ABA metabolism and signaling, which further impacts gibberellic acid signaling involving interactions between ABA and FUSCA3. Here we report that reduced auxin and ABA-signaling, in conjunction with increased cytokinin biosynthesis post-sectioning supports a model where interactions among hormones drives molecular networks leading to cell division, differentiation, and vegetative outgrowth. C1 [Anderson, James V.; Dogramaci, Muenevver; Horvath, David P.; Foley, Michael E.; Chao, Wun S.] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Suttle, Jeffrey C.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. [Thimmapuram, Jyothi; Hernandez, Alvaro G.] Univ Illinois, WM Keck Ctr Comparat & Funct Genom, Urbana, IL 61801 USA. [Ali, Shahjahan] Univ Sci & Technol KAUST, Biosci Core Lab Genom, Thuwal 239556900, Saudi Arabia. [Mikel, Mark A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Mikel, Mark A.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. RP Anderson, JV (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM james.anderson@ars.usda.gov OI Anderson, James/0000-0002-1801-5767; Horvath, David/0000-0002-8458-7691 NR 82 TC 11 Z9 11 U1 3 U2 32 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD AUG PY 2012 VL 12 IS 3 BP 515 EP 531 DI 10.1007/s10142-012-0280-5 PG 17 WC Genetics & Heredity SC Genetics & Heredity GA 998KY UT WOS:000308238900009 PM 22580957 ER PT J AU Campbell, MA Gleichsner, A Hilldorfer, L Horvath, D Suttle, J AF Campbell, Michael A. Gleichsner, Alyssa Hilldorfer, Lindsay Horvath, David Suttle, Jeffrey TI The sprout inhibitor 1,4-dimethylnaphthalene induces the expression of the cell cycle inhibitors KRP1 and KRP2 in potatoes SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Potato; Transcriptional analysis; Microarray; Sprout inhibitors; DMN; Dormancy ID DORMANCY; GROWTH; PROFILES; KINASE; ARABIDOPSIS; GENES; YEAST; CESSATION; MULTIPLE; PATHWAY AB The suppression of sprout growth is critical for the long-term storage of potato tubers. 1,4-Dimethylenapthlene (DMN) is a new class of sprout control agent but the metabolic mode of action for this compound has yet to be elucidated. Changes in transcriptional profiles of meristems isolated from potato tubers treated with the DMN were investigated using an Agilent 44 K 60-mer-oligo microarray. RNA was isolated from nondormant Russet Burbank meristems isolated from tubers treated with DMN for 3 days or activated charcoal as a control. RNA was used to develop probes that were hybridized against a microarray developed by the Potato Oligo Chip Initiative. Analysis of the array data was conducted in two stages: total array data was examined using a linear model and the software Limma and pathway analysis was conducted by linking the potato sequences to the Arabidopsis thaliana. DMN elicited a change in a number of transcripts associated with cold responses, water regulation, salt stress, and osmotic adjustment. DMN also resulted in a repression of cyclin or cyclin-like transcripts. DMN also resulted in a 50% decrease in thymidine incorporation suggesting a repression of the S phase of the cell cycle. Quantitative real-time polymerase chain reaction analysis demonstrated that DMN increased transcripts for the cell cycle inhibitors KRP1 and KRP2. We conclude the DMN results in alteration of genes associated with the maintenance of a G1/S phase block possibly through the induction of the cell cycle inhibitors KRP1 and KRP2. C1 [Campbell, Michael A.; Gleichsner, Alyssa; Hilldorfer, Lindsay] Penn State Univ, Behrend Coll, Sch Sci, Penn State Erie, Erie, PA 16563 USA. [Suttle, Jeffrey] ARS, USDA, Sugar Beet & Potato Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. [Horvath, David] ARS, USDA, Weed Biol Res Unit, Biosci Res Lab, Fargo, ND 58102 USA. RP Campbell, MA (reprint author), Penn State Univ, Behrend Coll, Sch Sci, Penn State Erie, P-1 Prischak Bldg,4205 Coll Dr, Erie, PA 16563 USA. EM mac17@psu.edu OI Horvath, David/0000-0002-8458-7691 FU 1,4-Group FX A portion of this work was supported through a grant from the 1,4-Group to MAC. NR 37 TC 1 Z9 1 U1 1 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD AUG PY 2012 VL 12 IS 3 BP 533 EP 541 DI 10.1007/s10142-011-0257-9 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA 998KY UT WOS:000308238900010 PM 22113341 ER PT J AU Silver, K Desormaux, A Freeman, LC Lillich, JD AF Silver, Kristopher Desormaux, Alejandra Freeman, Lisa C. Lillich, James D. TI Expression of pleiotrophin, an important regulator of cell migration, is inhibited in intestinal epithelial cells by treatment with non-steroidal anti-inflammatory drugs SO GROWTH FACTORS LA English DT Article DE Pleitrophin; NSAID; cell migration; epithelia ID MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION; TYROSINE-PHOSPHATASE BETA/ZETA; GROWTH-FACTOR; GASTROINTESTINAL TOXICITY; CYCLOOXYGENASE INHIBITION; SIGNALING PATHWAY; GASTRIC-MUCOSA; K+ CHANNELS; INDOMETHACIN; CALPAIN AB Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely used drugs for the suppression of inflammation and pain. However, the analgesic properties of NSAIDs are also associated with significant negative side effects, most notably in the gastrointestinal (GI) tract. Increasingly, evidence indicates that the ulcerogenic properties of some NSAIDs are not exclusively the result of inhibition of cyclooxygenase isoforms in the GI tract, and other mechanisms, including inhibition of cell migration and epithelial restitution, are being explored. Recently, microarray analysis was used to identify potential novel targets of NSAID activity in intestinal epithelial cells. Treated cells exhibited significant reductions in the gene expression of pleiotrophin (PTN), a cytokine and growth factor known to participate in angiogenesis and bone growth. This report aimed to confirm the microarray results reported previously, and to measure protein expression of PTN in intestinal epithelial cells. Furthermore, we also examined the effects of exogenous PTN on cell migration in the presence and absence of either NSAIDs with variable ulcerogenic potential or PTN-specific siRNA. Our results demonstrated that indomethacin and NS-398, two NSAIDs with ulcerogenic potential significantly decrease both gene and protein expressions of PTN in IEC-6 cells and protein expression in IEC-6-Cdx2 cells. Additionally, cell migration experiments with PTN siRNA showed that PTN is an important mediator of IEC-6 cell migration, and addition of exogenous PTN partially restores the deficits in cell migration caused by treatment with indomethacin and NS-398. Finally, measurement of PTN protein expression in the GI tract of horses treated with phenylbutazone showed that PTN expression is reduced by NSAIDs in vivo. Our results show that PTN is an important mediator of cell migration in IEC-6 cells, and PTN is a potential target through which NSAIDs may inhibit cell migration, epithelial restitution, and wound healing in the GI tract. C1 [Desormaux, Alejandra; Lillich, James D.] Kansas State Univ, Coll Vet Med, Dept Clin Sci, Manhattan, KS 66506 USA. [Silver, Kristopher] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Freeman, Lisa C.] Kansas State Univ, Dept Anat & Physiol, Coll Vet Med, Manhattan, KS 66506 USA. RP Lillich, JD (reprint author), Kansas State Univ, Coll Vet Med, Dept Clin Sci, Manhattan, KS 66506 USA. EM lillich@vet.k-state.edu OI Silver, Kristopher/0000-0002-8140-2786 FU National Institutes of Health Center of Biological Research Excellence/COBRE Mentoring Grant [NIH1P20RR017686] FX This work was supported by National Institutes of Health Center of Biological Research Excellence/COBRE Mentoring Grant No. NIH1P20RR017686 (Core A and B). The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 46 TC 6 Z9 6 U1 0 U2 10 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 0897-7194 J9 GROWTH FACTORS JI Growth Factors PD AUG PY 2012 VL 30 IS 4 BP 258 EP 266 DI 10.3109/08977194.2012.693920 PG 9 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 996UC UT WOS:000308119300005 PM 22691166 ER PT J AU Shin, S Torres-Acosta, JA Heinen, SJ McCormick, S Lemmens, M Paris, MPK Berthiller, F Adam, G Muehlbauer, GJ AF Shin, Sanghyun Torres-Acosta, Juan Antonio Heinen, Shane J. McCormick, Susan Lemmens, Marc Paris, Maria Paula Kovalsky Berthiller, Franz Adam, Gerhard Muehlbauer, Gary J. TI Transgenic Arabidopsis thaliana expressing a barley UDP-glucosyltransferase exhibit resistance to the mycotoxin deoxynivalenol SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Deoxynivalenol; Fusarium head blight; trichothecenes; UDP-glucosyltransferase ID FUSARIUM HEAD BLIGHT; TRANSCRIPTOME ANALYSIS; TRICHOTHECENE ACETYLTRANSFERASE; HETEROLOGOUS EXPRESSION; OVER-EXPRESSION; WHEAT SPIKES; GENE; OVEREXPRESSION; DETOXIFICATION; GRAMINEARUM AB Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of small grain cereal crops. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON). DON inhibits protein synthesis in eukaryotic cells and acts as a virulence factor during fungal pathogenesis, therefore resistance to DON is considered an important component of resistance against FHB. One mechanism of resistance to DON is conversion of DON to DON-3-O-glucoside (D3G). Previous studies showed that expression of the UDP-glucosyltransferase genes HvUGT13248 from barley and AtUGt73C5 (DOGT1) from Arabidopsis thaliana conferred DON resistance to yeast. Over-expression of AtUGt73C5 in Arabidopsis led to increased DON resistance of seedlings but also to dwarfing of transgenic plants due to the formation of brassinosteroid-glucosides. The objectives of this study were to develop transgenic Arabidopsis expressing HvUGT13248, to test for phenotypic changes in growth habit, and the response to DON. Transgenic lines that constitutively expressed the epitope-tagged HvUGT13248 protein exhibited increased resistance to DON in a seed germination assay and converted DON to D3G to a higher extent than the untransformed wild-type. By contrast to the over-expression of DOGT1 in Arabidopsis, which conjugated the brassinosteriod castasterone with a glucoside group resulting in a dwarf phenotype, expression of the barley HvUGT13248 gene did not lead to drastic morphological changes. Consistent with this observation, no castasterone-glucoside formation was detectable in yeast expressing the barley HvUGT13248 gene. This barley UGT is therefore a promising candidate for transgenic approaches aiming to increase DON and Fusarium resistance of crop plants without undesired collateral effects. C1 [Shin, Sanghyun; Heinen, Shane J.; Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Torres-Acosta, Juan Antonio; Paris, Maria Paula Kovalsky; Adam, Gerhard] Univ Res Ctr Tulln, A-3430 Tulln, Austria. [Torres-Acosta, Juan Antonio; Paris, Maria Paula Kovalsky; Adam, Gerhard] Univ Nat Resources & Life Sci, Dept Appl Genet & Cell Biol, Vienna, Austria. [Lemmens, Marc; Berthiller, Franz] Univ Nat Resources, Christian Doppler Lab Mycotoxin Metabolism & Bio, Ctr Analyt Chem, Dept Agrobiotechnol IFA Tulln, Tulln, Austria. [McCormick, Susan] ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP Muehlbauer, GJ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall,1991 Buford Circle, St Paul, MN 55108 USA. EM muehl003@umn.edu FU Austrian Science fund (SFB Fusarium) [F3701, F3706, F3708, L475-B09]; Federal Ministry of Economy, Family, and Youth; National Foundation for Research, Technology, and Development; National Institute of Crop Science (NICS), Rural Development Administration (RDA), Republic of Korea; Rural Development Administration, Republic of Korea [PJ00800602]; USDA-ARS US Wheat and Barley Scab Initiative; Minnesota Small Grains Initiative FX We would like to thank Dr Fumiaki Katagiri at the University of Minnesota for providing the pMDC32 plasmid. We are indebted to Abigail Cole and Anthony Jakubiak for excellent technical assistance. This project is supported by funds from the USDA-ARS US Wheat and Barley Scab Initiative and the Minnesota Small Grains Initiative to GJM. GA, ML, and FB received funding from the Austrian Science fund (SFB Fusarium, F3701, F3706, F3708 and L475-B09) and FB from the Federal Ministry of Economy, Family, and Youth as well as from the National Foundation for Research, Technology, and Development. Sanghyun Shin was supported by a postdoctoral fellowship programme of the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Republic of Korea, and a grant from the Next-Generation BioGreen21 program (No. PJ00800602), Rural Development Administration, Republic of Korea. NR 48 TC 35 Z9 36 U1 3 U2 55 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD AUG PY 2012 VL 63 IS 13 BP 4731 EP 4740 DI 10.1093/jxb/ers141 PG 10 WC Plant Sciences SC Plant Sciences GA 995LX UT WOS:000308010700005 PM 22922639 ER PT J AU Farnham, MW Lester, GE Hassell, R AF Farnham, Mark W. Lester, Gene E. Hassell, Richard TI Collard, mustard and turnip greens: Effects of genotypes and leaf position on concentrations of ascorbic acid, folate, beta-carotene, lutein and phylloquinone SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Brassica oleracea; Brassica juncea; Brassica rapa; Viridis; Cole crop; Leafy green; Vitamin C; Vitamin B-9; Vitamin K; Carotenoids; Biodiversity; Genotype difference and nutrition; Cultivar difference; Nutrient dense food; Food analysis; Food composition ID BRASSICA-OLERACEA; CULTIVARS; BROCCOLI; LANDRACES; FRUIT AB Leaf green Brassica crops: collard (Brassica oleracea L), mustard (Brassica juncea L) and turnip (Brassica rapa L) greens are important commercial and culinary vegetables, especially in the southern United States. However, almost no information on essential human-health vitamins [ascorbic acid (vitamin C), folate (vitamin B-9), phylloquinone (vitamin K-1) and the carotenoids lutein and beta-carotene (provitamin A)] is available. Leafy green Brassicas (15 collard, 2 mustard and 2 turnip greens) were harvested at peak whole-plant maturity, separated into younger (top-canopy) or older (bottom-canopy) leaves and assayed for the aforementioned vitamins and carotenoids. On a 100 g fresh mass basis, percent dry mass (14.7 g versus 13.2 g), total (132.7 mg versus 109.1 mg) and free (58.7 mg versus 52.8 mg) ascorbic acid, folate (183 mu g versus 112 mu g) and lutein (9790 mu g versus 8950 mu g) concentrations were significantly higher in younger versus older leaves. Phylloquinone (435 mu g versus 459 mu g) and beta-carotene (11,130 mu g versus 11,619 mu g) were equally concentrated in younger and older leaves. On a fresh mass basis, all vitamins were found to be highly concentrated in all nineteen leafy green Brassica genotypes, with particular genotypes within each Brassica species having exceptionally high concentrations. Findings from this study revealed that leafy green Brassica genotypes are nutritionally dense in essential human-health vitamins C, B-9, K, provitamin A (beta-carotene) and lutein. Concentration differences in the examined nutritional components among the genotypes (e.g. significant differences in lutein concentrations from 5120 to 15,360 mu g/100 gfm) reveal potential differences that might be exploited in genetic improvement through plant selection and breeding. Published by Elsevier Inc. C1 [Farnham, Mark W.] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Lester, Gene E.] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Hassell, Richard] Clemson Univ, Clemson Coastal Res & Educ Ctr, Charleston, SC 29414 USA. RP Farnham, MW (reprint author), ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. EM Mark.Farnham@ars.usda.gov NR 19 TC 7 Z9 7 U1 2 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2012 VL 27 IS 1 BP 1 EP 7 DI 10.1016/j.jfca.2012.04.008 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 988YC UT WOS:000307526400001 ER PT J AU Finotti, E Bersani, E Del Prete, E Friedman, M AF Finotti, Enrico Bersani, Enrico Del Prete, Ernesto Friedman, Mendel TI A functional mathematical index for predicting effects of food processing on eight sweet potato (Ipomoea batatas) cultivars SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Potato; Sweet potato; Ipomoea batatas; Functional mathematical index; Phenolic compounds; Cooking processes; Food processing; Nutrient retention; Food analysis; Food composition ID QUALITY; L. AB In this paper we apply an improved functional mathematical index (FMI), modified from those presented in previous publications, to define the influence of different cooking processes of eight sweet potato (Ipomoea batatas) cultivars on composition of six bioactive phenolic compounds (flavonoids). The index allows the evaluation of nutritional, safety, and processing aspects of the following six phenolic compounds: neochlorogenic acid (3-CQA), cryptochlorogenic acid (4-CQA), chlorogenic acid (5-CQA), and three isochlorogenic acids, namely isochlorogenic acid A (3,5-diCQA), isochlorogenic acid B (3,4-diCQA), and isochlorogenic acid C (4,5-diCQA). These sweet potato components are considered to be the most representative phenolic compounds in this widely consumed food. The results of the mathematical analysis suggest that the derived total and individual FMI values provide a tool for predicting the relative adverse effects of food-processing effects (boiling, deep frying, microwaving, oven baking, sauteing, and steaming) on the evaluated phenolic compounds of eight sweet potato varieties. The use of the FMI makes it possible to predict the effects of processing conditions on the content of phenolic compounds of new sweet potato varieties, without the need to actually subject the sweet potatoes to the various cooking conditions. (C) 2012 Elsevier Inc. All rights reserved. C1 [Finotti, Enrico] Ist Nazl Ric Alimenti & Nutr, I-00178 Rome, Italy. [Bersani, Enrico] Univ Roma La Sapienza, Lab Strutture & Mat Intelligenti, I-04012 Cisterna Di Larina, LT, Italy. [Del Prete, Ernesto] INAIL, I-00198 Rome, Italy. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Finotti, E (reprint author), Ist Nazl Ric Alimenti & Nutr, Via Ardetina 546, I-00178 Rome, Italy. EM finotti@inran.it OI Friedman, Mendel/0000-0003-2582-7517 NR 12 TC 1 Z9 1 U1 1 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2012 VL 27 IS 1 BP 81 EP 86 DI 10.1016/j.jfca.2012.04.001 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 988YC UT WOS:000307526400011 ER PT J AU Lester, GE Lewers, KS Medina, MB Saftner, RA AF Lester, Gene E. Lewers, Kim S. Medina, Marjorie B. Saftner, Robert A. TI Comparative analysis of strawberry total phenolics via Fast Blue BB vs. Folin-Ciocalteu: Assay interference by ascorbic acid SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Strawberries (Fragaria x ananassa Duch.); Diazonium; HPLC; Food analysis; Food composition; Assay for total phenolics; Bioactive non-nutrients; Fructose; Glucose; Sucrose ID ANTIOXIDANT CAPACITY; ANTHOCYANINS; GENOTYPES; ANANASSA; QUALITY; FLESH AB Unblemished fully ripe fruit from five day-neutral strawberry cultivars were harvested on two separate dates and evaluated for ascorbic acid (AsA), fruit sugars, and phenolic composition. Individual phenolics were determined by HPLC, and total phenolics by Folin-Ciocalteu (F-C) and by a 'new' assay: Fast Blue BB (FBBB), which detects phenolics directly. FBBB reported an average 2.9-fold greater concentration of total phenolics than F-C, had a significant correlation (r = 0.80; P = 0.001) with total phenolics via HPLC and did not interact with AsA or sugars, whereas F-C, an indirect detection assay for total phenolics, appeared to under-report total phenolic concentrations, had no significant correlation (r = 0.20) with total phenolics via HPLC or with sugars, but had a significant correlation (r = 0.64; P = 0.05) with total AsA. Results from this study indicated that previous studies of strawberry fruit, using the standard indirect F-C assay, have greatly underestimated the total phenolics content and that this assay should be replaced in future studies by the FBBB assay. Published by Elsevier Inc. C1 [Lester, Gene E.] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA,GEL, Beltsville, MD 20705 USA. [Lewers, Kim S.] ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Medina, Marjorie B.] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Lester, GE (reprint author), ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA,GEL, 10300 Baltimore Ave,Bldg 002, Beltsville, MD 20705 USA. EM gene.lester@ars.usda.gov FU USDA-ARS [1265-43440-004-00, 1275-21220-189-00] FX This project was funded by USDA-ARS Projects 1265-43440-004-00 and 1275-21220-189-00. The authors wish to thank Mr. John Enns and the BARC Research Support Services for establishing and maintaining the fields; Mr. Norman Livsey for evaluating the fruits and the anonymous reviewers for their helpful comments. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 22 TC 21 Z9 21 U1 6 U2 50 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2012 VL 27 IS 1 BP 102 EP 107 DI 10.1016/j.jfca.2012.05.003 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 988YC UT WOS:000307526400014 ER PT J AU Lee, CC Kibblewhite, RE Wagschal, K Li, RP Robertson, GH Orts, WJ AF Lee, Charles C. Kibblewhite, Rena E. Wagschal, Kurt Li, Ruiping Robertson, George H. Orts, William J. TI Isolation and characterization of a novel GH67 alpha-glucuronidase from a mixed culture SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE alpha-Glucuronidase; Library screening; Hemicellulose; Alkaline-active ID BACILLUS-STEAROTHERMOPHILUS T-6; FIBER CORCHORUS-CAPSULARIS; GEOBACILLUS-STEAROTHERMOPHILUS; ESTER LINKAGE; LIGNIN; HYDROLASES; XYLANASE; ENZYME; PULP AB Hemicelluloses represent a large reservoir of carbohydrates that can be utilized for renewable products. Hydrolysis of hemicellulose into simple sugars is inhibited by its various chemical substituents. The glucuronic acid substituent is removed by the enzyme alpha-glucuronidase. A gene (deg75-AG) encoding a putative alpha-glucuronidase enzyme was isolated from a culture of mixed compost microorganisms. The gene was subcloned into a prokaryotic vector, and the enzyme was overexpressed and biochemically characterized. The DEG75-AG enzyme had optimum activity at 45 A degrees C. Unlike other alpha-glucuronidases, the DEG75-AG had a more basic pH optimum of 7-8. When birchwood xylan was used as substrate, the addition of DEG75-AG increased hydrolysis twofold relative to xylanase alone. C1 [Lee, Charles C.; Kibblewhite, Rena E.; Wagschal, Kurt; Robertson, George H.; Orts, William J.] ARS, USDA, WRRC, Albany, CA 94710 USA. [Li, Ruiping] China Three Gorges Univ, Minist Educ, Engn Res Ctr Ecoenvironm Three Gorges Reservoir R, Yichang 443002, Peoples R China. RP Lee, CC (reprint author), ARS, USDA, WRRC, 800 Buchanan St, Albany, CA 94710 USA. EM Charles.Lee@ars.usda.gov NR 31 TC 2 Z9 2 U1 1 U2 10 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD AUG PY 2012 VL 39 IS 8 BP 1245 EP 1251 DI 10.1007/s10295-012-1128-7 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 985FH UT WOS:000307248300016 PM 22526332 ER PT J AU Amwayi, PW Masiga, DK Govender, P Teal, PEA Torto, B AF Amwayi, Peris W. Masiga, Daniel K. Govender, Prem Teal, Peter E. A. Torto, Baldwyn TI Mass spectral determination of phenylacetonitrile (PAN) levels in body tissues of adult desert locust, Schistocerca gregaria SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Schistocerca gregaria; Phenylacetonitrile; Pheromone; Locust; Mass spectrometry ID PHEROMONE; AGGREGATION; RELEASE; FORSKAL AB Wings and legs of the gregarious desert locust, Schistocerca gregaria have been shown to be release sites of phenylacetonitrile (PAN), the major adult male-produced pheromone. However, there is limited information on the distribution of PAN within the locust. Here we show, using gas chromatography-mass spectrometry (GC-MS), that PAN occurs in nearly all body parts of both adult males and females of the locust in varying amounts. PAN was 20-fold more concentrated in males than in females. In females, PAN was concentrated more in the tarsal segments. The greatest amounts of PAN were in 2- and 3-week old female and male body parts, respectively. No trace of PAN was found in similar ages and sexes of the solitarious phase desert locust. Our results show that PAN is distributed in the body matrix of both sexes of gregarious phase locusts and suggest that no specific tissue is responsible for biosynthesis of the pheromone. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Amwayi, Peris W.; Masiga, Daniel K.; Torto, Baldwyn] ICIPE, Nairobi 00100, Kenya. [Amwayi, Peris W.; Govender, Prem] Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa. [Govender, Prem] Univ Limpopo, Dept Biol, Limpopo, South Africa. [Teal, Peter E. A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Torto, B (reprint author), ICIPE, POB 30772, Nairobi 00100, Kenya. EM btorto@icipe.org OI Masiga, Daniel/0000-0001-7513-0887 FU USDA; DAAD; ARPPIS FX We thank USDA, DAAD and ARPPIS for supporting the research project at icipe. We acknowledge the assistance of various staff at the icipe BCED department. NR 19 TC 2 Z9 2 U1 0 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD AUG PY 2012 VL 58 IS 8 BP 1037 EP 1041 DI 10.1016/j.jinsphys.2012.03.012 PG 5 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 983YM UT WOS:000307154600001 PM 22609420 ER PT J AU Gregorc, A Evans, JD Scharf, M Ellis, JD AF Gregorc, Ales Evans, Jay D. Scharf, Mike Ellis, James D. TI Gene expression in honey bee (Apis mellifera) larvae exposed to pesticides and Varroa mites (Varroa destructor) SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Apis mellifera; Immunity; Detoxification; Pathogen; Pesticide ID IN-SITU LOCALIZATION; PAENIBACILLUS-LARVAE; CELL-DEATH; PEPTIDOGLYCAN RECOGNITION; HEAT-SHOCK; DROSOPHILA; COUMAPHOS; HEMOLYMPH; PROTEINS; COLONIES AB Honey bee (Apis mellifera) larvae reared in vitro were exposed to one of nine pesticides and/or were challenged with the parasitic mite, Varroa destructor. Total RNA was extracted from individual larvae and first strand cDNAs were generated. Gene-expression changes in larvae were measured using quantitative PCR (qPCR) targeting transcripts for pathogens and genes involved in physiological processes, bee health, immunity, and/or xenobiotic detoxification. Transcript levels for Peptidoglycan Recognition Protein (PGRPSC), a pathogen recognition gene, increased in larvae exposed to Varroa mites (P < 0.001) and were not changed in pesticide treated larvae. As expected, Varroa-parasitized brood had higher transcripts of Deformed Wing Virus than did control larvae (P < 0.001). Varroa parasitism, arguably coupled with virus infection, resulted in significantly higher transcript abundances for the antimicrobial peptides abaecin, hymenoptaecin, and defensin1. Transcript levels for Prophenoloxidase-activating enzyme (PPOact), an immune end product, were elevated in larvae treated with myclobutanil and chlorothalonil (both are fungicides) (P < 0.001). Transcript levels for Hexameric storage protein (Hsp70) were significantly upregulated in imidacloprid, fluvalinate, coumaphos, myclobutanil, and amitraz treated larvae. Definitive impacts of pesticides and Varroa parasitism on honey bee larval gene expression were demonstrated. Interactions between larval treatments and gene expression for the targeted genes are discussed. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Gregorc, Ales; Ellis, James D.] Univ Florida, Dept Entomol & Nematol, Honey Bee Res & Extens Lab, Gainesville, FL 32611 USA. [Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Scharf, Mike] Univ Florida, Dept Entomol & Nematol, Insect Toxicol Lab, Gainesville, FL 32611 USA. RP Gregorc, A (reprint author), Agr Inst Slovenia, Hacquetova 17, SI-1000 Ljubljana, Slovenia. EM ales.gregorc@kis.si RI Evans, Jay/C-8408-2012; Ellis, James/L-7985-2013 OI Evans, Jay/0000-0002-0036-4651; FU National Honey Board; North American Pollinator Protection Campaign; Florida Department of Agriculture and Consumer Services through Honey Bee Technical Council FX We thank Jeanette Klopchin, Michelle Kelley (University of Florida Honey Bee Research and Extension Laboratory) and Dawn L Lopez (USDA-ARS Bee Research Laboratory, Beltsville, MD) for their technical and molecular assistance with this project. This work was supported by the National Honey Board, the North American Pollinator Protection Campaign, and the Florida Department of Agriculture and Consumer Services through the work of the Honey Bee Technical Council. NR 63 TC 33 Z9 34 U1 9 U2 131 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD AUG PY 2012 VL 58 IS 8 BP 1042 EP 1049 DI 10.1016/j.jinsphys.2012.03.015 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 983YM UT WOS:000307154600002 PM 22497859 ER PT J AU Chaimanee, V Chantawannakul, P Chen, YP Evans, JD Pettis, JS AF Chaimanee, Veeranan Chantawannakul, Panuwan Chen, Yanping Evans, Jay D. Pettis, Jeffery S. TI Differential expression of immune genes of adult honey bee (Apis mellifera) after inoculated by Nosema ceranae SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Nosema ceranae; Antimicrobial peptides; Immunosuppression; Apis mellifera; Innate immunity ID COLONY LOSSES; DROSOPHILA-MELANOGASTER; INFECTION; MICROSPORIDIA; VITELLOGENIN; PHAGOCYTOSIS; PATHOGENS; PEPTIDE; EATER AB Nosema ceranae is a microsporidium parasite infecting adult honey bees (Apis mellifera) and is known to affects at both the individual and colony level. In this study, the expression levels were measured for four antimicrobial peptide encoding genes that are associated with bee humoral immunity (defensin, abaecin, apidaecin, and hymenoptaecin), eater gene which is a transmembrane protein involved cellular immunity and gene encoding female-specific protein (vitellogenin) in honey bees when inoculated by N. ceranae. The results showed that four of these genes, defensin, abaecin, apidaecin and hymenoptaecin were significantly down-regulated 3 and 6 days after inoculations. Additionally, antimicrobial peptide expressions did not significantly differ between control and inoculated bees after 12 days post inoculation. Moreover, our results revealed that the mRNA levels of eater and vitellogenin did not differ significantly following N. ceranae inoculation. Therefore, in this study we reaffirmed that N. ceranae infection induces host immunosuppression. Published by Elsevier Ltd. C1 [Chen, Yanping; Evans, Jay D.; Pettis, Jeffery S.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Chaimanee, Veeranan; Chantawannakul, Panuwan] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Ctr, Chiang Mai 50200, Thailand. [Chantawannakul, Panuwan] Chiang Mai Univ, Fac Sci, Mat Sci Res Ctr, Chiang Mai 50200, Thailand. RP Pettis, JS (reprint author), USDA ARS, Bee Res Lab, Bldg 476 BARC E, Beltsville, MD 20705 USA. EM jeff.pettis@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU Thailand Research Fund; National Research University; Office of Higher Education Commission; USDA-ARS Bee Research Laboratory; program of Strategic Scholarships for Frontier Research Network for the Joint Ph.D. Program FX V.C. was supported by a grant under the program of Strategic Scholarships for Frontier Research Network for the Joint Ph.D. Program Thai Doctoral degree from the Office of the Higher Education Commission, Thailand. We thank the Thailand Research Fund, National Research University, Office of Higher Education Commission and the USDA-ARS Bee Research Laboratory for financial support. We also thank M. Hamilton, D. Lopez, V. Levi and N. Rice for their excellent technical help with the project. NR 33 TC 48 Z9 49 U1 2 U2 52 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD AUG PY 2012 VL 58 IS 8 BP 1090 EP 1095 DI 10.1016/j.jinsphys.2012.04.016 PG 6 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 983YM UT WOS:000307154600007 PM 22609362 ER PT J AU Choi, MY Meer, RKV Coy, M Scharf, ME AF Choi, Man-Yeon Meer, Robert K. Vander Coy, Monique Scharf, Michael E. TI Phenotypic impacts of PBAN RNA interference in an ant, Solenopsis invicta, and a moth, Helicoverpa zea SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE PBAN; Neuropeptide; RNAi; dsRNA; Fire ant; Hymenoptera; Corn earworm; Lepidoptera; Pest control ID BIOSYNTHESIS ACTIVATING NEUROPEPTIDE; GENE-ENCODING DH; DIAPAUSE HORMONE; MOLECULAR CHARACTERIZATION; FIRE ANT; SUBESOPHAGEAL GANGLION; INSECT NEUROPEPTIDES; BOMBYX-MORI; EXPRESSION; IDENTIFICATION AB Insect neuropeptide hormones represent more than 90% of all insect hormones. The PBAN/pyrokinin family is a major group of insect neuropeptides, and they are expected to be found from all insect groups. These species-specific neuropeptides have been shown to have a variety of functions from embryo to adult. PBAN is well understood in moth species relative to sex pheromone biosynthesis, but other potential functions are yet to be determined. Recently, we focused on defining the PBAN gene and peptides in fire ants in preparation for an investigation of their function(s). RNA interference (RNAi) technology is a convenient tool to investigate unknown physiological functions in insects, and it is now an emerging method for development of novel biologically-based control agents as alternatives to insecticides. This could be a paradigm shift that will avoid many problems associated with conventional chemical insecticides. In this study, we selected the PBAN gene and its neuropeptide products as an RNAi target from two insect groups; a social insect, the fire ant (Solenopsis invicta) and a non-social insect, the corn earworm (Helicoverpa zea). Both insects are economically important pests. We report negative impacts after PBAN dsRNA treatment to suppress PBAN gene transcription during developmental and adult stages of both species, e.g. increased adult and larval mortality, delayed pupal development and decreased sex pheromone production in the moth. This is an important first step in determining the multiple functions of the PBAN gene in these two insects. This work illustrates the variety of phenotypic effects observed after RNAi silencing of the PBAN gene and suggests the possibility of novel biologically-based insect pest control methods. Published by Elsevier Ltd. C1 [Choi, Man-Yeon; Meer, Robert K. Vander] USDA ARS, CMAVE, Gainesville, FL 32608 USA. [Coy, Monique; Scharf, Michael E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32608 USA. [Scharf, Michael E.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Choi, MY (reprint author), USDA ARS, CMAVE, 1600 SW,23rd Dr, Gainesville, FL 32608 USA. EM mychoi@ars.usda.gov; bob.vandermeer@ars.usda.gov OI Choi, Man-Yeon/0000-0003-0769-380X NR 37 TC 17 Z9 17 U1 3 U2 34 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD AUG PY 2012 VL 58 IS 8 BP 1159 EP 1165 DI 10.1016/j.jinsphys.2012.06.005 PG 7 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 983YM UT WOS:000307154600015 PM 22705256 ER PT J AU Harshburger, BJ Walden, VP Humes, KS Moore, BC Blandford, TR Rango, A AF Harshburger, Brian J. Walden, Von P. Humes, Karen S. Moore, Brandon C. Blandford, Troy R. Rango, Albert TI Generation of Ensemble Streamflow Forecasts Using an Enhanced Version of the Snowmelt Runoff Model SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE snow hydrology; water supply; surface water hydrology; quantitative modeling; ensemble streamflow forecasting ID PREDICTION SYSTEM; PRECIPITATION; BASIN AB Harshburger, Brian J., Von P. Walden, Karen S. Humes, Brandon C. Moore, Troy R. Blandford, and Albert Rango, 2012. Generation of Ensemble Streamflow Forecasts Using an Enhanced Version of the Snowmelt Runoff Model. Journal of the American Water Resources Association (JAWRA) 48(4): 643-655. DOI: 10.1111/j.1752-1688.2012.00642.x Abstract: As water demand increases in the western United States, so does the need for accurate streamflow forecasts. We describe a method for generating ensemble streamflow forecasts (1-15 days) using an enhanced version of the snowmelt runoff model (SRM). Forecasts are produced for three snowmelt-dominated basins in Idaho. Model inputs are derived from meteorological forecasts, snow cover imagery, and surface observations from Snowpack Telemetry stations. The model performed well at lead times up to 7 days, but has significant predictability out to 15 days. The timing of peak flow and the streamflow volume are captured well by the model, but the peak-flow value is typically low. The model performance was assessed by computing the coefficient of determination (R2), percentage of volume difference (Dv%), and a skill score that quantifies the usefulness of the forecasts relative to climatology. The average R2 value for the mean ensemble is >0.8 for all three basins for lead times up to seven days. The Dv% is fairly unbiased (within +/- 10%) out to seven days in two of the basins, but the model underpredicts Dv% in the third. The average skill scores for all basins are >0.6 for lead times up to seven days, indicating that the ensemble model outperforms climatology. These results validate the usefulness of the ensemble forecasting approach for basins of this type, suggesting that the ensemble version of SRM might be applied successfully to other basins in the Intermountain West. C1 [Harshburger, Brian J.] Aniuk Consulting LLC, Kenosha, WI 53142 USA. [Walden, Von P.; Humes, Karen S.; Moore, Brandon C.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Blandford, Troy R.] State Montana, Dept Water Resources, Helena, MT USA. [Rango, Albert] ARS, USDA, Las Cruces, NM USA. RP Harshburger, BJ (reprint author), Aniuk Consulting LLC, 7105 61st Ave, Kenosha, WI 53142 USA. EM brian.harshburger@aniukconsulting.com FU Pacific Northwest Regional Collaboratory as part of Raytheon Corporation; NASA [NAS5-03098, 110] FX The authors thank the Natural Resources Conservation Service, United States Geological Survey, and the National Snow and Ice Data Center for providing the data required to complete this study. This research was supported by the Pacific Northwest Regional Collaboratory as part of Raytheon Corporation's Synergy project, funded by NASA through NAS5-03098, Task No. 110. This grant also funded the graduate research for Brian Harshburger (Ph.D.), Brandon Moore (M.S.), and Troy Blandford (M.S.). NR 25 TC 4 Z9 4 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2012 VL 48 IS 4 BP 643 EP 655 DI 10.1111/j.1752-1688.2012.00642.x PG 13 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 982AL UT WOS:000307014000001 ER PT J AU Hunsaker, CT Whitaker, TW Bales, RC AF Hunsaker, Carolyn T. Whitaker, Thomas W. Bales, Roger C. TI Snowmelt Runoff and Water Yield Along Elevation and Temperature Gradients in California's Southern Sierra Nevada SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE forests; snow hydrology; watershed management; runoff ratio; Sierra Nevada; mountain hydrology; headwaters ID SUB-ALPINE FOREST; VEGETATION CHANGES; TIMBER HARVEST; STREAMFLOW; CATCHMENT; AUGMENTATION; MANAGEMENT; DYNAMICS; SCALE; CREEK AB Hunsaker, Carolyn T., Thomas W. Whitaker, and Roger C. Bales, 2012. Snowmelt Runoff and Water Yield Along Elevation and Temperature Gradients in Californias Southern Sierra Nevada. Journal of the American Water Resources Association (JAWRA) 48(4): 667-678. DOI: 10.1111/j.1752-1688.2012.00641.x Abstract: Differences in hydrologic response across the rain-snow transition in the southern Sierra Nevada were studied in eight headwater catchments the Kings River Experimental Watersheds using continuous precipitation, snowpack, and streamflow measurements. The annual runoff ratio (discharge divided by precipitation) increased about 0.1 per 300 m of mean catchment elevation over the range 1,800-2,400 m. Higher-elevation catchments have lower vegetation density, shallow soils with rapid permeability, and a shorter growing season when compared with those at lower elevations. Average annual temperatures ranged from 6.8 degrees C at 2,400 m to 8.6 at 1,950 m elevation, with annual precipitation being 75-95% snow at the highest elevations vs. 20-50% at the lowest. Peak discharge lagged peak snow accumulation on the order of 60 days at the higher elevations and 20 to 30 days at the lower elevations. Snowmelt dominated the daily streamflow cycle over a period of about 30 days in higher elevation catchments, followed by a 15-day transition to evapotranspiration dominating the daily streamflow cycle. Discharge from lower elevation catchments was rainfall dominated in spring, with the transition to evapotranspiration dominance being less distinct. Climate warming that results in a longer growing season and a shift from snow to rain would result in earlier runoff and a lower runoff ratio. C1 [Hunsaker, Carolyn T.] US Forest Serv, Pacific SW Res Stn, USDA, Fresno, CA 93710 USA. [Whitaker, Thomas W.] Infin Wind Power, Santa Barbara, CA USA. [Bales, Roger C.] Univ Calif, Sierra Nevada Res Inst, Merced, CA USA. RP Hunsaker, CT (reprint author), US Forest Serv, Pacific SW Res Stn, USDA, 2081 E Sierra Ave, Fresno, CA 93710 USA. EM chunsaker@fs.fed.us OI Hunsaker, Carolyn/0000-0002-5662-2402 FU USDA Forest Service; California's State Water Resources Control Board, through Proposition 50; National Science Foundation, through the Southern Sierra Critical Zone Observatory [EAR-0725097] FX The establishment of KREW would not have been possible without funding from the National Fire Plan of the USDA Forest Service. Parts of the research received support from California's State Water Resources Control Board, through Proposition 50 (the Water Security, Clean Drinking Water, Coastal, and Beach Protection Act of 2002), and the National Science Foundation, through the Southern Sierra Critical Zone Observatory (EAR-0725097). Sean Eagan and Jeff Anderson, the first KREW hydrologists, deserve much of the credit for the design and establishment of the flumes and meteorology stations. Thomas Whitaker was a hydrologist with the Forest Service at the time this work was performed. Sage advice was provided by many hydrologists/scientists in the early years, including Randall Osterhuber, Charles Troendle, John Potyondy, and Brian Staab. Mention of a brand name does not constitute endorsement. NR 28 TC 30 Z9 31 U1 5 U2 58 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2012 VL 48 IS 4 BP 667 EP 678 DI 10.1111/j.1752-1688.2012.00641.x PG 12 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 982AL UT WOS:000307014000003 ER PT J AU Qiu, Z Dosskey, MG AF Qiu, Z. Dosskey, M. G. TI Multiple function benefit - Cost comparison of conservation buffer placement strategies SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Conservation buffers; Multiple criteria; Soil erodibility; Hydrological sensitivity; Wildlife habitat; Impervious surface ID WATER-QUALITY BUFFERS; CRITICAL SOURCE AREAS; AGRICULTURAL LANDSCAPES; RIPARIAN BUFFERS; LAND RETIREMENT; UNITED-STATES; MANAGEMENT; POLLUTION; URBANIZATION; STREAMS AB Conservation buffers are considered to be effective practices for repairing impaired streams and restoring multiple ecosystem functions in degraded agricultural watersheds. Six different planning strategies for targeting their placement within watersheds were compared in terms of cost-effectiveness for environmental improvement in the 144 km(2) Neshanic River Watershed in New Jersey, USA. The strategies included two riparian-focused strategies, two soil survey-based strategies and two topography-based strategies that focus traditionally on water quality benefits. Each strategy was used to prioritize locations to install conservation buffers. An analytical methodology was employed to evaluate the level of multiple benefits (water quality improvement, erosion control, wildlife habitat improvement, and stormwater mitigation) and buffer establishment and maintenance costs provided by each strategy. The comparison results showed that the riparian-focused strategies were least cost-effective (their cost-effectiveness measure ranges from 0.17 to 0.18) compared to both soil survey-based and topography-based buffer targeting strategies (from 0.21 to 0.31). Although the riparian-focused strategies are popular and simple to administer, alternative placement strategies should be considered when riparian-focused strategies cannot meet the environmental goals, additional environmental concerns are involved and the program cost is of a great concern. The appropriate strategies to compare, the specific evaluation criteria, and the proper scoring system depend upon specific land characteristics and issues that are important in a given watershed. Specific comparative results may not be directly transferable to other watersheds or planning areas, but the methodological framework developed can be a useful tool for planners to compare alternative multiple-function buffer strategies. (C) 2012 Elsevier B.V. All rights reserved. C1 [Qiu, Z.] New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA. [Dosskey, M. G.] USDA Forest Serv, Natl Agroforestry Ctr, Lincoln, NE USA. RP Qiu, Z (reprint author), New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA. EM zeyuan.qiu@njit.edu; mdosskey@fs.fed.us FU U.S. Department of Agriculture, Forest Service, Southern Research Station, National Agroforestry Center [09-DG-11330152-057]; Office of Policy Implementation and Watershed Restoration at New Jersey Department of Environmental Protection [RP06-068]; National Center for Environmental Research at the U.S. Environmental Protection Agency [RD-83336301-0] FX The funding support to this study was partially provided by the U.S. Department of Agriculture, Forest Service, Southern Research Station, National Agroforestry Center (grant number 09-DG-11330152-057), the Office of Policy Implementation and Watershed Restoration at New Jersey Department of Environmental Protection (grant number RP06-068) and the National Center for Environmental Research at the U.S. Environmental Protection Agency (grant number RD-83336301-0). The authors are grateful to two anonymous reviewers for their helpful comments in improving the overall quality of the manuscript. NR 66 TC 8 Z9 8 U1 1 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD AUG PY 2012 VL 107 IS 2 BP 89 EP 99 DI 10.1016/j.landurbplan.2012.05.001 PG 11 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 983UX UT WOS:000307145300003 ER PT J AU Hadsell, DL Wei, J Olea, W Hadsell, LA Renwick, A Thomson, PC Shariflou, M Williamson, P AF Hadsell, D. L. Wei, J. Olea, W. Hadsell, L. A. Renwick, A. Thomson, P. C. Shariflou, M. Williamson, P. TI In silico QTL mapping of maternal nurturing ability with the mouse diversity panel SO PHYSIOLOGICAL GENOMICS LA English DT Article DE genome-wide association study; lactation; mouse; haplotype ID QUANTITATIVE TRAIT LOCI; MILK-PRODUCTION TRAITS; MAMMARY-GLAND DEVELOPMENT; DAIRY-CATTLE; INBRED MICE; GENOME SCAN; LACTATION PERSISTENCY; POPULATION-STRUCTURE; PROLONGED LACTATION; HOLSTEIN POPULATION AB Hadsell DL, Wei J, Olea W, Hadsell LA, Renwick A, Thomson PC, Shariflou M, Williamson P. In silico QTL mapping of maternal nurturing ability with the mouse diversity panel. Physiol Genomics 44: 787-798, 2012. First published July 3, 2012; doi:10.1152/physiolgenomics.00159.2011.-Significant variation exists for maternal nurturing ability in inbred mice. Although classical mapping approaches have identified quantitative trait loci (QTL) that may account for this variation, the underlying genes are unknown. In this study, lactation performance data among the mouse diversity panel were used to map genomic regions associated with this variation. Females from each of 32 inbred strains (n = 8-19 dams/strain) were studied during the first 8 days of lactation by allowing them to raise weight-and size-normalized cross-foster litters (10 pups/litter). Average daily weight gain (ADG) of litters served as the primary indicator of milk production. The number of pups successfully reared to 8 days (PNUM8) also served as a related indicator of maternal performance. Initial haplotype association analysis using a Bonferroni-corrected, genome-wide threshold revealed 10 and 15 associations encompassing 11 and 13 genes for ADG and PNUM8, respectively. The most significant of these associated haplotype blocks were found on MMU 8, 11, and 19 and contained the genes Nr3c2, Egfr, Sec61g, and Gnaq. Lastly, two haplotype blocks on MMU9 were detected in association with PNUM8. These overlapped with the previously described maternal performance QTL, Neogq1. These results suggest that the application of in silico QTL mapping is a useful tool in discovering the presence of novel candidate genes involved in determining lactation capacity in mice. C1 [Hadsell, D. L.; Olea, W.; Hadsell, L. A.; Renwick, A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Hadsell, D. L.] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Wei, J.; Thomson, P. C.; Shariflou, M.; Williamson, P.] Univ Sydney, Fac Vet Sci, Sydney, NSW 2006, Australia. RP Hadsell, DL (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St,Mail Stop BCM 320, Houston, TX 77030 USA. EM dhadsell@bcm.edu RI Wei, Jerry/B-7385-2013 OI Wei, Jerry/0000-0001-8802-7221 FU National Institute of Child Health and Human Development [1R21HD-059746-01A1]; USDA/ARS [6250-51000-054] FX This work was funded by grants from the National Institute of Child Health and Human Development Grant #1R21HD-059746-01A1 and the USDA/ARS (cooperative agreement #6250-51000-054). NR 79 TC 3 Z9 3 U1 0 U2 4 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1094-8341 J9 PHYSIOL GENOMICS JI Physiol. Genomics PD AUG PY 2012 VL 44 IS 16 BP 787 EP 798 DI 10.1152/physiolgenomics.00159.2011 PG 12 WC Cell Biology; Genetics & Heredity; Physiology SC Cell Biology; Genetics & Heredity; Physiology GA 996GS UT WOS:000308073500001 PM 22759921 ER PT J AU Xu, M Fitzgerald, SD Zhang, HM Karcher, DM Heidari, M AF Xu, Ming Fitzgerald, Scott D. Zhang, Huanmin Karcher, Darrin M. Heidari, Mohammad TI Very Virulent Plus Strains of MDV Induce an Acute Form of Transient Paralysis in Both Susceptible and Resistant Chicken Lines SO VIRAL IMMUNOLOGY LA English DT Article ID MAREKS-DISEASE VIRUS; NERVOUS-SYSTEM; PROINFLAMMATORY CYTOKINES; GENE-EXPRESSION; HERPESVIRUS; INFECTION; ENCEPHALITIS; BRAIN; CELLS; INTERLEUKIN-10 AB Marek's disease (MD) is a lymphoproliferative disease of domestic chickens caused by a highly cell-associated alpha herpesvirus, Marek's disease virus (MDV). Clinical signs of MD include depression, crippling, weight loss, and transient paralysis (TP). TP is a disease of the central nervous system that affects MD-susceptible chickens 8-11 days post-infection (dpi), normally resulting in recovery 1-3 d after the onset of clinical signs. In this study we inoculated chickens from lines 7(2) (MD-susceptible) and 6(3) (MD-resistant) with a very virulent plus strain of MDV at 2wk of age, and collected brain samples from birds with and without TP at 5, 11, and 21 dpi for gene expression profiling and histological analysis. Data revealed that chickens inoculated with MDV had higher levels of IL-6, IL-10, IL-18, IFN-alpha, IFN-beta, IFN-gamma, MHC I, and CD18 in their brains at 11 dpi compared to the uninfected control birds. In addition, the expression levels of IL-6, IL-10, IFN-alpha, IFN-beta, and IFN-gamma were significantly higher in the brains of the birds showing clinical signs of TP than in asymptomatic chickens. Comparative analysis between the two chicken lines showed that the expression levels of IL-6, IL-10, IFN-beta, IFN-gamma, IL-18, CD18, and MHC I were significantly higher in the brains of the birds from line 6(3) with TP than those of line 7(2) exhibiting neurological disorders. A differential expression pattern was observed for some of the tested genes at different time points post-inoculation. Histological analysis showed lymphocytic meningitis, perivascular cuffing, and neuronal degeneration within the brains of birds from both susceptible and resistant lines exhibiting TP at 11 dpi. Vaccination prevented development of TP and other MD-associated clinical symptoms. These observations are suggestive of an underlying immunological mechanism for viral-induced neurological dysfunction, and the differential responses of the two chicken lines to MDV infection. C1 [Xu, Ming; Zhang, Huanmin; Heidari, Mohammad] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Xu, Ming] Jilin Univ, Coll Anim & Vet Sci, Changchun 130023, Peoples R China. [Xu, Ming] Acad Mil Med Sci, Inst Mil Vet, Changchun, Peoples R China. [Xu, Ming; Karcher, Darrin M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Fitzgerald, Scott D.] Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA. [Fitzgerald, Scott D.] Michigan State Univ, Diagnost Ctr Populat & Anim Hlth, E Lansing, MI 48824 USA. RP Heidari, M (reprint author), USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM mohammad.heidari@ars.usda.gov RI Xu, Ming/B-9896-2015 NR 36 TC 3 Z9 3 U1 0 U2 15 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 0882-8245 J9 VIRAL IMMUNOL JI Viral Immunol. PD AUG PY 2012 VL 25 IS 4 BP 306 EP 323 DI 10.1089/vim.2012.0003 PG 18 WC Immunology; Virology SC Immunology; Virology GA 991UZ UT WOS:000307729400010 PM 22803780 ER PT J AU Ma, XJ Lin, YZ Lin, LG Qin, GJ Pereira, FA Haymond, MW Butte, NF Sun, YX AF Ma, Xiaojun Lin, Yuezhen Lin, Ligen Qin, Guijun Pereira, Fred A. Haymond, Morey W. Butte, Nancy F. Sun, Yuxiang TI Ablation of ghrelin receptor in leptin-deficient ob/ob mice has paradoxical effects on glucose homeostasis when compared with ablation of ghrelin in ob/ob mice SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE growth hormone secretagogue receptor; insulin secretion; type 2 diabetes ID HORMONE SECRETAGOGUE RECEPTOR; DES-ACYL GHRELIN; REGULATES INSULIN-SECRETION; PANCREATIC ALPHA-CELLS; DIET-INDUCED OBESITY; UNCOUPLING PROTEIN-2; RAT PANCREAS; FOOD-INTAKE; BETA-CELLS; ENERGY-EXPENDITURE AB Ma X, Lin Y, Lin L, Qin G, Pereira FA, Haymond MW, Butte NF, Sun Y. Ablation of ghrelin receptor in leptin-deficient ob/ob mice has paradoxical effects on glucose homeostasis when compared with ablation of ghrelin in ob/ob mice. Am J Physiol Endocrinol Metab 303: E422-E431, 2012. First published June 5, 2012; doi: 10.1152/ajpendo.00576.2011.-The orexigenic hormone ghrelin is important in diabetes because it has an inhibitory effect on insulin secretion. Ghrelin ablation in leptin-deficient ob/ob (Ghrelin(-/-):ob/ob) mice increases insulin secretion and improves hyperglycemia. The physiologically relevant ghrelin receptor is the growth hormone secretagogue receptor (GHS-R), and GHS-R antagonists are thought to be an effective strategy for treating diabetes. However, since some of ghrelin's effects are independent of GHS-R, we have utilized genetic approaches to determine whether ghrelin's effect on insulin secretion is mediated through GHS-R and whether GHS-R antagonism indeed inhibits insulin secretion. We investigated the effects of GHS-R on glucose homeostasis in Ghsr-ablated ob/ob mice (Ghsr(-/-):ob/ob). Ghsr ablation did not rescue the hyperphagia, obesity, or insulin resistance of ob/ob mice. Surprisingly, Ghsr ablation worsened the hyperglycemia, decreased insulin, and impaired glucose tolerance. Consistently, Ghsr ablation in ob/ob mice upregulated negative beta-cell regulators (such as UCP-2, SREBP-1c, ChREBP, and MIF-1) and downregulated positive beta-cell regulators (such as HIF-1 alpha, FGF-21, and PDX-1) in whole pancreas; this suggests that Ghsr ablation impairs pancreatic beta-cell function in leptin deficiency. Of note, Ghsr ablation in ob/ob mice did not affect the islet size; the average islet size of Ghsr(-/-):ob/ob mice is similar to that of ob/ob mice. In summary, because Ghsr ablation in leptin deficiency impairs insulin secretion and worsens hyperglycemia, this suggests that GHS-R antagonists may actually aggravate diabetes under certain conditions. The paradoxical effects of ghrelin ablation and Ghsr ablation in ob/ob mice highlight the complexity of the ghrelin-signaling pathway. C1 [Ma, Xiaojun; Lin, Ligen; Haymond, Morey W.; Butte, Nancy F.; Sun, Yuxiang] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Ma, Xiaojun; Qin, Guijun] Zhengzhou Univ, Affiliated Hosp 1, Dept Internal Med, Div Endocrinol, Zhengzhou, Herts, Peoples R China. [Lin, Yuezhen; Haymond, Morey W.] Baylor Coll Med, Texas Childrens Hosp, Dept Pediat Endocrinol & Metab, Houston, TX 77030 USA. [Pereira, Fred A.; Sun, Yuxiang] Baylor Coll Med, Dept Mol & Cellular Biol, Huffington Ctr Aging, Houston, TX 77030 USA. RP Sun, YX (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates Ave,Rm 5024, Houston, TX 77030 USA. EM yuxiangs@bcm.edu OI Pereira, Fred/0000-0003-2100-0280 FU USDA/ARS [58-6250-0-008]; NIH/NIA [1-R03-AG-029641-01]; American Heart Association [12IRG9230004]; NIH-Diabetes and Endocrinology Research Center grant at Baylor College of Medicine [P30-DK-079638]; Lipid Core of Mouse Metabolic Phenotyping Center at Vanderbilt University [U24-DK-59637] FX This work is a publication of the US Department of Agriculture/Agricultural Research Service (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, and has been funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. This work was also supported by NIH/NIA Grant 1-R03-AG-029641-01 (Y. Sun), the American Heart Association 12IRG9230004 (Y. Sun), an NIH-Diabetes and Endocrinology Research Center grant at Baylor College of Medicine (P30-DK-079638), and the Lipid Core of Mouse Metabolic Phenotyping Center at Vanderbilt University (U24-DK-59637). NR 60 TC 13 Z9 13 U1 0 U2 6 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD AUG PY 2012 VL 303 IS 3 BP E422 EP E431 DI 10.1152/ajpendo.00576.2011 PG 10 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 984YC UT WOS:000307228700012 PM 22669248 ER PT J AU Mohammad, MA Hadsell, DL Haymond, MW AF Mohammad, Mahmoud A. Hadsell, Darryl L. Haymond, Morey W. TI Gene regulation of UDP-galactose synthesis and transport: potential rate-limiting processes in initiation of milk production in humans SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE fat globule; lactose synthesis; glucose metabolism; microarray ID MOUSE MAMMARY-GLAND; ALPHA-LACTALBUMIN; LACTOSE SYNTHESIS; SECRETORY ACTIVATION; GLUCOSE-TRANSPORTER; LACTATING WOMEN; PROGESTERONE WITHDRAWAL; HORMONAL-REGULATION; EPITHELIAL-CELLS; TIGHT JUNCTIONS AB Mohammad MA, Hadsell DL, Haymond MW. Gene regulation of UDP-galactose synthesis and transport: potential rate-limiting processes in initiation of milk production in humans. Am J Physiol Endocrinol Metab 303: E365-E376, 2012. First published May 29, 2012; doi:10.1152/ajpendo.00175.2012.-Lactose synthesis is believed to be rate limiting for milk production. However, understanding the molecular events controlling lactose synthesis in humans is still rudimentary. We have utilized our established model of the RNA isolated from breast milk fat globule from seven healthy, exclusively breastfeeding women from 6 h to 42 days following delivery to determine the temporal coordination of changes in gene expression in the carbohydrate metabolic processes emphasizing the lactose synthesis pathway in human mammary epithelial cell. We showed that milk lactose concentrations increased from 75 to 200 mM from 6 to 96 h. Milk progesterone concentrations fell by 65% at 24 h and were undetectable by day 3. Milk prolactin peaked at 36 h and then declined progressively afterward. In concordance with lactose synthesis, gene expression of galactose kinase 2, UDP-glucose pyrophosphorylase 2 (UGP2), and phosphoglucomutase 1 increased 18-, 10-, and threefold, respectively, between 6 and 72 h. Between 6 and 96 h, gene expression of UDP-galactose transporter 2 (SLC35A2) increased threefold, whereas glucose transporter 1 was unchanged. Gene expression of lactose synthase no. 3 increased 1.7-fold by 96 h, whereas alpha-lactalbumin did not change over the entire study duration. Gene expression of prolactin receptor (PRLR) and its downstream signal transducer and activator of transcription complex 5 (STAT5) were increased 10- and 2.5-fold, respectively, by 72 h. In summary, lactose synthesis paralleled the induction of gene expression of proteins involved in UDP-galactose synthesis and transport, suggesting that they are potentially rate limiting in lactose synthesis and thus milk production. Progesterone withdrawal may be the signal that triggers PRLR signaling via STAT5, which may in turn induce UGP2 and SLC35A2 expression. C1 [Mohammad, Mahmoud A.; Hadsell, Darryl L.; Haymond, Morey W.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Haymond, MW (reprint author), USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM mhaymond@bcm.edu OI Mohammad, Mahmoud/0000-0002-6535-5529 FU National Institutes of Health [RO1-DK-55478, HD-37857, MO1-RR-00188, USDA/ARS 6250-5100]; NIH-NIDDK-RO1 FX This project was supported by National Institutes of Health Grants RO1-DK-55478, HD-37857, MO1-RR-00188, and USDA/ARS 6250-5100. This work is a publication of the US Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX). The contents of this publication do not necessarily reflect the views of policies of the US Department of Agriculture, nor does the mention of trade names, commercial products, or organizations imply endorsement from the US Government.; The authors declare that there is no conflict of interest that would prejudice the impartiality of the scientific work. M. A. Mahmoud and D. L. Hadsell have nothing to declare. M. W. Haymond received an NIH-NIDDK-RO1 award for this study. NR 61 TC 14 Z9 15 U1 2 U2 10 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD AUG PY 2012 VL 303 IS 3 BP E365 EP E376 DI 10.1152/ajpendo.00175.2012 PG 12 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 984YC UT WOS:000307228700007 PM 22649065 ER PT J AU Anderson, DM Fredrickson, EL Estell, RE AF Anderson, D. M. Fredrickson, E. L. Estell, R. E. TI Managing livestock using animal behavior: mixed-species stocking and flerds SO ANIMAL LA English DT Article DE livestock; bonding; predation; animal behavior ID GRAZING GRASS/FORB PASTURES; WHITE-TAILED DEER; RED DEER; DIETARY OVERLAP; VOLUNTARY INTAKE; SEED PLANTS; MULE DEER; SHEEP; CATTLE; GOATS AB Mixed-species stocking can foster sound landscape management while offering economic and ecological advantages compared with mono-species stocking. Producers contemplating a mixed-species enterprise should reflect on several considerations before implementing this animal management strategy. Factors applicable to a particular producer's landscape must be considered together with goals and economic constraints before implementing mixed-species stocking. A major consideration when using mixed-species stocking is how to deal with predation losses, especially among small ruminants. An approach being adopted in some commercial operations capitalizes on using innate animal behaviors to form cohesive groups of two or more livestock species that consistently remain together under free-ranging conditions. These groups are referred to as flerds. The mixing of a flock of sheep and/or goats with a herd of cattle into a flerd has been shown to protect sheep and goats from coyote predation, as well as offering other husbandry advantages. Some of the added advantages include more efficient conversion of forage into animal protein. Creation of flerds, their maintenance and advantages are discussed. C1 [Anderson, D. M.; Estell, R. E.] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Fredrickson, E. L.] Eastern Kentucky Univ, Dept Agr, Richmond, KY 40475 USA. RP Anderson, DM (reprint author), USDA ARS Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. EM deanders@nmsu.edu NR 182 TC 4 Z9 4 U1 2 U2 24 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 J9 ANIMAL JI Animal PD AUG PY 2012 VL 6 IS 8 BP 1339 EP 1349 DI 10.1017/S175173111200016X PG 11 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 980DB UT WOS:000306872700017 PM 23217238 ER PT J AU Dodds, WK Robinson, CT Gaiser, EE Hansen, GJA Powell, H Smith, JM Morse, NB Johnson, SL Gregory, SV Bell, T Kratz, TK McDowell, WH AF Dodds, Walter K. Robinson, Christopher T. Gaiser, Evelyn E. Hansen, Gretchen J. A. Powell, Heather Smith, Joseph M. Morse, Nathaniel B. Johnson, Sherri L. Gregory, Stanley V. Bell, Tisza Kratz, Timothy K. McDowell, William H. TI Surprises and Insights from Long-Term Aquatic Data Sets and Experiments SO BIOSCIENCE LA English DT Article DE data; lakes; streams; freshwater; long term ID CRAYFISH ORCONECTES-RUSTICUS; ECOLOGICAL RESEARCH; CLIMATE-CHANGE; ENVIRONMENTAL-CHANGE; PUERTO-RICO; LAKE; ECOSYSTEM; RIVER; FLORIDA; FOREST AB Long-term research on freshwater ecosystems provides insights that can be difficult to obtain from other approaches. Widespread monitoring of ecologically relevant water-quality parameters spanning decades can facilitate important tests of ecological principles. Unique long-term data sets and analytical tools are increasingly available, allowing for powerful and synthetic analyses across sites. Long-term measurements or experiments in aquatic systems can catch rare events, changes in highly variable systems, time-lagged responses, cumulative effects of stressors, and biotic responses that encompass multiple generations. Data are available from formal networks, local to international agencies, private organizations, various institutions, and paleontological and historic records; brief literature surveys suggest much existing data are not synthesized. Ecological sciences will benefit from careful maintenance and analyses of existing long-term programs, and subsequent insights can aid in the design of effective future long-term experimental and observational efforts. Long-term research on freshwaters is particularly important because of their value to humanity. C1 [Dodds, Walter K.] Kansas State Univ, Manhattan, KS 66506 USA. [Robinson, Christopher T.] Swiss Fed Inst Aquat Sci & Technol, Dubendorf, Switzerland. [Gaiser, Evelyn E.] Florida Int Univ, University Pk, PA USA. [Gaiser, Evelyn E.] Archbold Biol Stn, Highlands Cty, FL USA. [Hansen, Gretchen J. A.] Univ Wisconsin, Madisons Ctr Linmol, Madison, WI 53706 USA. [Powell, Heather] Natl Ecol Observ Network, Boulder, CO USA. [Smith, Joseph M.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Morse, Nathaniel B.] Univ New Hampshire, Nat Resources & Earth Syst Sci Program, Durham, NH 03824 USA. [Johnson, Sherri L.] US Forest Serv, Pacific NW Res Stn, Portland, OR 97208 USA. [Gregory, Stanley V.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Bell, Tisza] Montana State Univ, Bozeman, MT 59717 USA. [Kratz, Timothy K.] Univ Wisconsin, Ctr Linmol, Trout Lake Stn, Madison, WI USA. [McDowell, William H.] Univ New Hampshire, Dept Environm Sci, Durham, NH 03824 USA. [McDowell, William H.] Univ New Hampshire, New Hampshire Water Resources Res Ctr, Durham, NH 03824 USA. RP Dodds, WK (reprint author), Kansas State Univ, Manhattan, KS 66506 USA. EM wkdodds@ksu.edu RI McDowell, William/E-9767-2010 OI McDowell, William/0000-0002-8739-9047 FU National Science Foundation (NSF) [0919443]; NSF LTER [1026843, 0822700, 0823341, 0620910, 0823380, 0832652] FX We appreciate all the individuals who helped collect the data presented in this article. We thank the Long Term Ecological Research (LTER) Network office for enabling our group to meet in Estes Park in 2009 and the National Science Foundation (NSF) for funding (grant no. 0919443 to WKD), and support was also provided by the following NSF LTER grants: no. 1026843 at the Toolik Lake site, no. 0822700 at the North Temperate Lakes site, no. 0823341 at the Konza Prairie site, no. 0620910 at the Luquillo Experimental Forest, no. 0823380 at the H. J. Andrews Experimental Forest, no. 0832652 at the Niwot Ridge site. The Lake Annie data were supported by Archbold Biological Station. This is contribution no. 12-437-J from the Kansas Agricultural Experiment Station. NR 62 TC 18 Z9 18 U1 8 U2 71 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD AUG PY 2012 VL 62 IS 8 BP 709 EP 721 DI 10.1525/bio.2012.62.8.4 PG 13 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 990JF UT WOS:000307626100004 ER PT J AU Schwartz, MW Hellmann, JJ McLachlan, JM Sax, DF Borevitz, JO Brennan, J Camacho, AE Ceballos, G Clark, JR Doremus, H Early, R Etterson, JR Fielder, D Gill, JL Gonzalez, P Green, N Hannah, L Jamieson, DW Javeline, D Minteer, BA Odenbaugh, J Polasky, S Richardson, DM Root, TL Safford, HD Sala, O Schneider, SH Thompson, AR Williams, JW Vellend, M Vitt, P Zellmer, S AF Schwartz, Mark W. Hellmann, Jessica J. McLachlan, Jason M. Sax, Dov F. Borevitz, Justin O. Brennan, Jean Camacho, Alejandro E. Ceballos, Gerardo Clark, Jamie R. Doremus, Holly Early, Regan Etterson, Julie R. Fielder, Dwight Gill, Jacquelyn L. Gonzalez, Patrick Green, Nancy Hannah, Lee Jamieson, Dale W. Javeline, Debra Minteer, Ben A. Odenbaugh, Jay Polasky, Stephen Richardson, David M. Root, Terry L. Safford, Hugh D. Sala, Osvaldo Schneider, Stephen H. Thompson, Andrew R. Williams, John W. Vellend, Mark Vitt, Pati Zellmer, Sandra TI Managed Relocation: Integrating the Scientific, Regulatory, and Ethical Challenges SO BIOSCIENCE LA English DT Article DE ethics; policy; law; conservation; translocation ID CLIMATE-CHANGE; ASSISTED COLONIZATION; CHANGING CLIMATE; BIODIVERSITY CONSERVATION; SPECIES DISTRIBUTIONS; DISTRIBUTION MODELS; FUTURE; ECOLOGY; ECOSYSTEMS; INVASIONS AB Managed relocation is defined as the movement of species, populations, or genotypes to places outside the areas of their historical distributions to maintain biological diversity or ecosystem functioning with changing climate. It has been claimed that a major extinction event is under way and that climate change is increasing its severity Projections indicating that climate change may drive substantial losses of biodiversity have compelled some scientists to suggest that traditional management strategies are insufficient. The managed relocation of species is a controversial management response to climate change. The published literature has emphasized biological concerns over difficult ethical, legal, and policy issues. Furthermore, ongoing managed relocation actions lack scientific and societal engagement. Our interdisciplinary team considered ethics, law, policy, ecology, and natural resources management in order to identify the key issues of managed relocation relevant for developing sound policies that support decisions for resource management. We recommend that government agencies develop and adopt best practices for managed relocation. C1 [Schwartz, Mark W.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Hellmann, Jessica J.; McLachlan, Jason M.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Sax, Dov F.] Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. [Borevitz, Justin O.] Australian Natl Univ, Res Sch Biol, Canberra, ACT, Australia. [Brennan, Jean] Virginia Polytech Inst & State Univ, Conservat Management Inst, Blacksburg, VA 24061 USA. [Camacho, Alejandro E.] Univ Calif Irvine, Sch Law, Irvine, CA USA. [Camacho, Alejandro E.] Univ Calif Irvine, Ctr Land Environm & Nat Resources, Irvine, CA USA. [Ceballos, Gerardo] Univ Nacl Autonoma Mexico, Inst Ecol, Mexico City 04510, DF, Mexico. [Clark, Jamie R.] Defenders Wildlife, Washington, DC USA. [Doremus, Holly] Univ Calif Berkeley, Sch Law, Berkeley, CA 94720 USA. [Early, Regan] Univ Evora, Rui Nabeiro Biodivers Chair, Ctr Invest Biodiversidade & Recursos Genet, Evora, Portugal. [Etterson, Julie R.] Univ Minnesota Duluth, Dept Biol, Duluth, MN USA. [Fielder, Dwight] US Bur Land Management, Washington, DC USA. [Gill, Jacquelyn L.; Williams, John W.] Univ Wisconsin Madison, Dept Geog, Madison, WI USA. [Gill, Jacquelyn L.; Williams, John W.] Univ Wisconsin Madison, Nelson Ctr Climat Res, Madison, WI USA. [Gonzalez, Patrick] Natl Pk Serv, Nat Resource Stewardship & Sci Directorate, Washington, DC USA. [Green, Nancy] US Fish & Wildlife Serv, Arlington, VA USA. [Hannah, Lee] Conservat Int Ctr Appl Biodivers Sci, Santa Barbara, CA USA. [Jamieson, Dale W.] NYU, Environm Studies Program, New York, NY USA. [Javeline, Debra] Univ Notre Dame, Dept Polit Sci, Notre Dame, IN 46556 USA. [Minteer, Ben A.; Sala, Osvaldo] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Minteer, Ben A.; Sala, Osvaldo] Arizona State Univ, Sch Sustainabil, Tempe, AZ USA. [Odenbaugh, Jay] Lewis & Clark Coll, Dept Philosophy, Portland, OR 97219 USA. [Polasky, Stephen] Univ Minnesota, Dept Appl Econ, St Paul, MN 55108 USA. [Richardson, David M.] Univ Stellenbosch, Ctr Invas Biol, Dept Bot & Zool, ZA-7600 Stellenbosch, South Africa. [Root, Terry L.] Stanford Univ, Woods Inst Environm, Stanford, CA 94305 USA. [Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Safford, Hugh D.] US Forest Serv, Valejo, CA USA. [Schneider, Stephen H.] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. [Thompson, Andrew R.] Natl Ocean & Atmospher Adm, SW Fisheries Sci Ctr, La Jolla, CA USA. [Vellend, Mark] Univ Sherbrooke, Dept Biol, Sherbrooke, PQ J1K 2R1, Canada. [Vitt, Pati] Chicago Bot Garden, Chicago, IL USA. [Zellmer, Sandra] Univ Nebraska, Coll Law, Lincoln, NE USA. RP Schwartz, MW (reprint author), Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. EM mwschwartz@ucdavis.edu RI Schwartz, Mark/G-1066-2011; Richardson, David/A-1495-2008; Gonzalez, Patrick/B-9479-2013; Borevitz, Justin/B-5423-2012; OI Schwartz, Mark/0000-0002-3739-6542; Richardson, David/0000-0001-9574-8297; Gonzalez, Patrick/0000-0002-7105-0561; Camacho, Alejandro/0000-0002-8782-6870; Early, Regan/0000-0003-4108-5904; Vitt, Pati/0000-0002-3727-9178 FU National Science Foundation [DEB 0741792, DEB 0741921]; Cedar Tree Foundation FX This article is dedicated to Stephen H. Schneider, who was a member of the Managed Relocation Working Group and a tireless advocate for careful deliberation of climate change issues involving both scientific analysis and social values. He was a giant in our field and a friend and mentor to many of the authors of this article. This work was supported by National Science Foundation grants no. DEB 0741792 and no. DEB 0741921 in support of the Managed Relocation Working Group and by a Cedar Tree Foundation grant. NR 65 TC 75 Z9 76 U1 15 U2 175 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD AUG PY 2012 VL 62 IS 8 BP 732 EP 743 DI 10.1525/bio.2012.62.8.6 PG 12 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 990JF UT WOS:000307626100006 ER PT J AU Gotardo, AT Schumaher, BH Pfister, JA Traldi, AS Maiorka, PC Spinosa, HS Gorniak, SL AF Gotardo, Andre T. Schumaher, Breno H. Pfister, James A. Traldi, Anneliese S. Maiorka, Paulo C. Spinosa, Helenice S. Gorniak, Silvana L. TI The Use of Ultrasonography to Study Teratogenicity in Ruminants: Evaluation of Ipomoea carnea in Goats SO BIRTH DEFECTS RESEARCH PART B-DEVELOPMENTAL AND REPRODUCTIVE TOXICOLOGY LA English DT Article DE ultrasonagraphy; morning glory; teratology; reproductive toxicology livestock; swainsonine ID LOCOWEED ASTRAGALUS-LENTIGINOSUS; LYSOSOMAL STORAGE; FETAL DEVELOPMENT; SHEEP; CONVOLVULACEAE; INTOXICATION; SWAINSONINE; ULTRASOUND; INGESTION; TOXICITY AB Background: Ipomoea carnea (I. carnea) is a poisonous plant found in Brazil and other tropical countries that often poison livestock. The plant contains the alkaloids calystegines and mainly swainsonine, which inhibit cellular enzymes and cause systematic cell death. The objective of this study was to evaluate the perinatal effects of I. carnea in goats. Methods: Forty-seven pregnant goats were randomly allocated into 5 treatment groups and given the following doses (g/kg BW) of I. carnea: 0 (IC0), 1.0 (IC1), 3.0 (IC3), 5.0 (IC5) and 7.5 (IC7). The treatment animals were given fresh I. carnea from day 27 of gestation to parturition. Weight gains and serum biochemistry were evaluated. Fetuses were evaluated using ultrasonographic measurements. Results: Goats from the IC7 group showed clinical signs of poisoning. Ultrasound examination revealed that I. carnea feeding in all treatment groups reduced fetal movement compared to the controls. There was an increase in the total number of birth defects (retrognathia and arthrogyposis) in the IC7 and IC5 groups compared to the controls. Conclusion: The results show that I. carnea has teratogenic potential in goats. In addition, ultrasounds were useful in evaluating fetotoxicity and teratogenicity. Birth Defects Res (Part B) 00:17, 2012. (c) 2012 Wiley Periodicals, Inc. C1 [Gotardo, Andre T.; Maiorka, Paulo C.; Spinosa, Helenice S.; Gorniak, Silvana L.] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Res Ctr Vet Toxicol CEPTOX, BR-05508900 Sao Paulo, Brazil. [Schumaher, Breno H.] Sch Vet Med, Super Baptist Sch Amazon ESBAM, Manaus, Amazonas, Brazil. [Pfister, James A.] ARS, USDA, Poisonous Plant Res Lab, Logan, UT USA. [Traldi, Anneliese S.] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-05508900 Sao Paulo, Brazil. RP Gorniak, SL (reprint author), Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Res Ctr Vet Toxicol CEPTOX, Prof Dr Orlando Marques de Paiva Ave 87, BR-05508900 Sao Paulo, Brazil. EM gorniak@usp.br RI Gorniak, Silvana/C-7879-2013; Gotardo, Andre/G-3788-2013; Souza Ferreira, Maria/J-5108-2015; Spinosa, Helenice/E-1170-2012 OI Gotardo, Andre/0000-0001-7297-0522; Spinosa, Helenice/0000-0003-1953-0477 FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [2006/58729-2] FX We thank Paulo Cesar Fabricio Raspantini, Leonila Ester Reinert Raspantini, Estevao Belloni, Marco Antonio Faustino dos Santos, and Adilson Baladore for their valuable assistance with the study and for the animal care. We thank Dr. Dale Gardner for the analysis of swainsonine. This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP-(Proc no. 2006/58729-2). The authors declare that they have no competing interests. NR 55 TC 4 Z9 4 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-9733 J9 BIRTH DEFECTS RES B JI Birth Defects Res. Part B-Dev. Reprod. Toxicol. PD AUG PY 2012 VL 95 IS 4 BP 289 EP 295 DI 10.1002/bdrb.21017 PG 7 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA 986YJ UT WOS:000307383300003 PM 22645093 ER PT J AU Dardick, C Schwessinger, B Ronald, P AF Dardick, Chris Schwessinger, Benjamin Ronald, Pamela TI Non-arginine-aspartate (non-RD) kinases are associated with innate immune receptors that recognize conserved microbial signatures SO CURRENT OPINION IN PLANT BIOLOGY LA English DT Review ID STEM RUST-RESISTANCE; RICH REPEAT RECEPTOR; PROTEIN-KINASE; DEFENSE RESPONSES; LEUCINE-RICH; INTERLEUKIN-1 RECEPTOR; PSEUDOMONAS-SYRINGAE; FLAGELLIN PERCEPTION; XA21-MEDIATED IMMUNITY; ARABIDOPSIS-THALIANA AB An important question in the field of plant-pathogen interactions is how the detection of pathogens is converted into an effective immune response. In recent years, substantial insight has been gained into the identities of both the plant receptors and the microbial molecules they recognize. Likewise, many of the downstream signaling proteins and transcriptions factors that activate defense responses have been characterized. However, the early molecular events that comprise 'recognition' and how defense signaling specificity is achieved are not as well understood. In this review we discuss the significance of non-arginine-aspartate (non-RD) kinases, a subclass of kinases that are often found in association with pattern recognition receptors (PRRs). C1 [Dardick, Chris] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Schwessinger, Benjamin; Ronald, Pamela] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Schwessinger, Benjamin; Ronald, Pamela] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. RP Dardick, C (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM chris.dardick@ars.usda.gov OI Schwessinger, Benjamin/0000-0002-7194-2922 FU NIH [GM59962] FX We would like to acknowledge all of our other colleagues whose work we were unable to include but has substantially contributed to our understanding of PRR kinases. NIH GM59962 provided support for this project. NR 80 TC 29 Z9 31 U1 1 U2 32 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5266 EI 1879-0356 J9 CURR OPIN PLANT BIOL JI Curr. Opin. Plant Biol. PD AUG PY 2012 VL 15 IS 4 BP 358 EP 366 DI 10.1016/j.pbi.2012.05.002 PG 9 WC Plant Sciences SC Plant Sciences GA 996AE UT WOS:000308055600003 PM 22658367 ER PT J AU Padhi, SK Haas, M Bornscheuer, UT AF Padhi, Santosh Kumar Haas, Michael Bornscheuer, Uwe T. TI Lipase-catalyzed transesterification to remove saturated MAG from biodiesel SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Biodiesel; Fatty acid methyl ester; Glycerol monopalmitate; Glycerol monostearate; Lipase-G; MAG ID MONOGLYCERIDES AB Saturated MAG (SMG) are known to be present in FAME intended to be used as biodiesel. These SMG can strongly affect the properties of biofuels such as the cloud point (CP), and they have been implicated in engine failure due to filter plugging. It is shown here that lipase G from Penicillium camemberti can be efficiently used for the transesterification of SMG to fatty acid methyl ester and glycerol even in the presence of the bulk biodiesel. Thus, in samples of commercial biodiesel to which glycerol monostearate (GMS) and glycerol monopalmitate (GMP) had been added, their levels were enzymatically reduced from 2% (w/v) to 0.22% (w/v) for GMP and 0.14% (w/v) for GMS as confirmed by GC-MS analysis. Practical applications: SMG present in biodiesel can have a pronounced negative effect on the CP, and/or filterability and in-field performance of the fuel. The lipase-catalyzed transesterification shown in this paper enables a significant reduction in the amount of SMG, leading to superior biodiesel quality. C1 [Padhi, Santosh Kumar; Bornscheuer, Uwe T.] Ernst Moritz Arndt Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, D-17487 Greifswald, Germany. [Haas, Michael] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. RP Bornscheuer, UT (reprint author), Ernst Moritz Arndt Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, Felix Hausdorff Str 4, D-17487 Greifswald, Germany. EM uwe.bornscheuer@uni-greifswald.de RI Bornscheuer, Uwe/C-4612-2012 OI Bornscheuer, Uwe/0000-0003-0685-2696 FU Alexander von Humboldt Foundation (AvH) Germany FX We thank the Alexander von Humboldt Foundation (AvH) Germany, for a fellowship to SKP and Anita Gollin (Institute of Biochemistry, Greifswald, Germany) for help with GMP synthesis. NR 18 TC 0 Z9 0 U1 0 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD AUG PY 2012 VL 114 IS 8 BP 875 EP 879 DI 10.1002/ejlt.201100417 PG 5 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 991UJ UT WOS:000307727400005 ER PT J AU Grubman, MJ Segundo, FDS Dias, CCA Moraes, MP Perez-Martin, E de los Santos, T AF Grubman, Marvin J. Segundo, Fayna Diaz-San Dias, Camila C. A. Moraes, Mauro P. Perez-Martin, Eva de los Santos, Teresa TI Use of replication-defective adenoviruses to develop vaccines and biotherapeutics against foot-and-mouth disease SO FUTURE VIROLOGY LA English DT Review DE foot-and-mouth disease; interferon; replication-defective human adenovirus; vaccine ID NONSTRUCTURAL PROTEIN 2B; VIRUS SUBUNIT VACCINE; HOST IMMUNE-RESPONSE; TOLL-LIKE RECEPTORS; T-CELL RESPONSE; ANTIVIRAL ACTIVITY; INTERFERON-ALPHA; III INTERFERON; MESSENGER-RNA; FMDV PEPTIDES AB We have developed a replication-defective human adenovirus (Ad5) vectored foot-and-mouth disease (FMD) vaccine platform that protects both swine and cattle from subsequent challenge with homologous virus after a single immunization. This Ad5-FMD vaccine has undergone testing following the requirements of the Center for Veterinary Biologics of the Animal Plant and Health Inspection Service, US Department of Agriculture, and has recently been granted a conditional license for inclusion of the vaccine in the US National Veterinary Vaccine Stockpile. In this review, we will describe the approaches we have taken to improve the potency and efficacy of this vaccine platform. Furthermore, we will discuss the development of Ad5 vector-based biotherapeutics to generate rapid protection against FMD virus prior to vaccine-induced adaptive immunity and describe the use of a combination of these approaches to stimulate both fast and long-lasting immunity. C1 [Grubman, Marvin J.; Segundo, Fayna Diaz-San; Dias, Camila C. A.; Moraes, Mauro P.; Perez-Martin, Eva; de los Santos, Teresa] ARS, Plum Isl Anim Dis Ctr, N Atlantic Area, USDA, Greenport, NY 11944 USA. [Dias, Camila C. A.; Perez-Martin, Eva] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN 37831 USA. [Moraes, Mauro P.] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. [Moraes, Mauro P.] Ceva Biomune, Shawnee Mission, KS 66215 USA. RP Grubman, MJ (reprint author), ARS, Plum Isl Anim Dis Ctr, N Atlantic Area, USDA, Greenport, NY 11944 USA. FU CRIS [1940-32000-053-00D]; ARS; USDA through Science and Technology Directorate of the US Department of Homeland Security [HSHQPD-07-X-00003, HSHQDC-09-X-00373, HSHQDC-11-X-00189]; USDA through National Pork Board [11-005, 12-023] FX This research was supported in part by CRIS project number 1940-32000-053-00D, ARS, USDA (T de los Santos and MJ Grubman), grants through an interagency agreement with the Science and Technology Directorate of the US Department of Homeland Security under the Award Numbers HSHQPD-07-X-00003, HSHQDC-09-X-00373, HSHQDC-09-X-00373 and HSHQDC-11-X-00189 (T de los Santos and MJ Grubman) and through National Pork Board Grants #11-005 and #12-023 (T de los Santos, F Diaz-San Segundo and MJ Grubman). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. NR 84 TC 7 Z9 7 U1 0 U2 6 PU FUTURE MEDICINE LTD PI LONDON PA UNITEC HOUSE, 3RD FLOOR, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON, N3 1QB, ENGLAND SN 1746-0794 J9 FUTURE VIROL JI Future Virol. PD AUG PY 2012 VL 7 IS 8 BP 767 EP 778 DI 10.2217/FVL.12.65 PG 12 WC Virology SC Virology GA 986DR UT WOS:000307321700009 ER PT J AU Goodman, CL Stanley, D Ringbauer, JA Beeman, RW Silver, K Park, Y AF Goodman, Cynthia L. Stanley, David Ringbauer, Joseph A., Jr. Beeman, Richard W. Silver, Kristopher Park, Yoonseong TI A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Article DE Tribolium castaneum; Coleoptera; Beetle; Cell line; Insect genomics ID INSECT; EXPRESSION; CHRYSOMELIDAE; ESTABLISHMENT; GENES; TIME AB The red flour beetle, Tribolium castaneum, is a model organism for agricultural and medical research and its complete genome is sequenced. We established a continuously replicating T. castaneum cell line to complement existing physiological, genetic, and genomic research tools. We set up trial cell cultures from egg, pupa, and adult stages as tissue sources and incubated them in six separate cell culture media to determine the optimal combination of tissue source and medium for cell replication. Our most promising culture was generated by co-culturing adult (similar to 75 %) and pupal tissues in EX-CELL 420 medium containing 9 % FBS. Our new cell culture is designated BCIRL-TcA-CLG1 (TcA) and it has been subcultured more than 90 times. Amplification of genomic DNA with species-specific primers yielded DNA fragments of the expected sizes and with sequences identical to those from the published Tribolium genome. Additionally, we characterized this line using DNA fingerprinting (DAF-PCR) and compared it with three other coleopteran cell lines and its conspecific pupae to confirm identity. Its doubling time is 155.2 hr. Early passages consisted of attached cells and vesicles in suspension, whereas later passages consisted primarily of attached, spherical cells. Similar to other established cell lines, the ploidy of TcA cells was variable, ranging from 20 chromosomes/cell (diploid) to above 30 chromosomes/cell. TcA cells withstood incubation at 40A degrees C for 1 h with no decrease in viability. We recorded increased levels of one heat shock protein (43 kDa) and of the hsp68a transcript following exposure to 40A degrees C. Taken together, this represents the first report of a continuously replicating T. castaneum cell line. We expect the BCIRL-TcA-CLG1 line will become a useful tool in Tribolium research. C1 [Goodman, Cynthia L.; Stanley, David; Ringbauer, Joseph A., Jr.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. [Beeman, Richard W.; Silver, Kristopher] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. [Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Goodman, CL (reprint author), ARS, Biol Control Insects Res Lab, USDA, 1503 S Providence Rd, Columbia, MO 65203 USA. EM Cindy.Goodman@ars.usda.gov; David.Stanley@ars.usda.gov; Joseph.Ringbauer@ars.usda.gov; Richard.Beeman@ars.usda.gov; Kristopher.Silver@ars.usda.gov; ypark@ksu.edu RI Park, Yoonseong/J-5861-2013; OI Park, Yoonseong/0000-0003-1191-7335; Silver, Kristopher/0000-0002-8140-2786 NR 34 TC 12 Z9 13 U1 1 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD AUG PY 2012 VL 48 IS 7 BP 426 EP 433 DI 10.1007/s11626-012-9524-x PG 8 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 988SB UT WOS:000307509900006 PM 22752637 ER PT J AU Kirker, GT Wagner, TL Diehl, SV AF Kirker, Grant T. Wagner, Terence L. Diehl, Susan V. TI Relationship between wood-inhabiting fungi and Reticulitermes spp. in four forest habitats of northeastern Mississippi SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Wood biodeterioration; Coarse-woody debris; Wood fungi; Subterranean termites ID SUBTERRANEAN TERMITES ISOPTERA; RHINOTERMITIDAE; RESPONSES; BLOCKS AB Fungi from coarse woody debris samples containing or lacking termites were isolated, and identified from upland and bottomland hardwoods and pines in northeast Mississippi. Samples yielded 860 unique fungal isolates, with 59% identified to genus level. Four phyla, six classes, 10 orders, 14 families, and 50 genera were recovered. The fungal groups encountered by decreasing taxonomic diversity were Imperfect Fungi, Ascomycota, Zygomycota, Basidiomycota, and unknown fungi. The most frequently encountered fungi were Penicillium (81 occurrences), Nodulisporium (57), Cladosporium (37), Trichoderma (34), Xylaria (29), Talaromyces and Pestalotia (27 each), and Stachylidium (26). The true wood decay fungi only accounted for 0.9% of the fungi isolated. The only statistical interactions associated with termites were the genus Nodulisporium, the class Coelomycetes, and the order Xylariales which all correlated with the absence of termites. Of particular interest is the strong correlation of the Xylariales and absence of termites. These white rot ascomycetes may have inhibitory effects on termites. In addition, the correlation of the genus Nodulisporium may be related as many species of this genus are considered asexual stages of Xylaria and Hypoxylon. There were also a number of significant interactions between wood species, habitat and presence of certain fungi. Most prior research has found an attraction of termites to wood infested with different types of wood-associated fungi. This study, however, found no positive statistical correlations between the presence of termites and any given group of fungi. An increased understanding of these interactions may help locate and isolate biologically active compounds that may influence termite behavior. Published by Elsevier Ltd.. C1 [Kirker, Grant T.; Diehl, Susan V.] Mississippi State Univ, Coll Forest Resources, Forest & Wildlife Res Ctr, Dept Forest Prod, Mississippi State, MS 39759 USA. [Wagner, Terence L.] USDA, Forest Serv, Termite Res Unit, Starkville, MS 39760 USA. RP Kirker, GT (reprint author), USDA, Forest Prod Lab, Forest Serv, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM gkirker@fs.fed.us; twagner01@fs.fed.us; sdiehl@cfr.msstate.edu OI Kirker, Grant/0000-0001-6073-6624 FU USDA Forest Service FX The authors wish to thank the USDA Forest Service for funding this graduate research. Additional thanks are owed to E.L. Scruggs at the Wood Products Insect Research Unit for technical assistance in culture and isolation of wood inhabiting fungi. This publication has been peer reviewed and accepted as a Mississippi State University Forestry and Wildlife Research Center publication (FWRC publication FP643). NR 52 TC 6 Z9 10 U1 3 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD AUG PY 2012 VL 72 BP 18 EP 25 DI 10.1016/j.ibiod.2012.04.011 PG 8 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA 990EI UT WOS:000307613400003 ER PT J AU van den Berg, AK Perkins, TD Isselhardt, ML Godshall, MA Lloyd, SW AF van den Berg, A. K. Perkins, T. D. Isselhardt, M. L. Godshall, M-A. Lloyd, S. W. TI Maple syrup production with sap concentrated to high levels by membrane separation: Effects on syrup chemical composition and flavor SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE evaporator; maple sap; maple syrup; membrane separation; sugar maple AB Concentration of maple sap by membrane separation to greater than 20 degrees Brix prior to thermal evaporation could significantly increase the profitability of maple syrup production by reducing fuel costs and increasing the yield of high-value, light-colored syrup. However the effects of this practice on the overall quality of maple syrup, particularly on the unique flavor characteristics which underlie this sweetener's high economic value, are unknown. The objective of this study was to determine if the chemical composition and flavor of syrup produced from sap concentrated to 21.5 degrees Brix differed significantly from those of syrup produced from the same sap concentrated to 8 degrees Brix, the level currently utilized by maple producers. Maple syrup produced from sap concentrated to 21.5 degrees Brix was significantly lighter in color than syrup produced simultaneously from the same sap concentrated to 8 degrees Brix (p < 0.02). However, there were no other significant differences observed in the properties, composition, or perceived flavor of the two types of syrup. The results indicate that producing syrup with sap concentrated to 21.5 degrees Brix does not significantly impact syrup quality, and thus that this is an acceptable practice producers can use to increase the profitability of maple syrup production. C1 [van den Berg, A. K.; Perkins, T. D.; Isselhardt, M. L.] Univ Vermont, Proctor Maple Res Ctr, Underhill Ctr, VT 05490 USA. [Godshall, M-A.] Sugar Proc Res Inst Inc, New Orleans, LA 70124 USA. [Lloyd, S. W.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP van den Berg, AK (reprint author), Univ Vermont, Proctor Maple Res Ctr, POB 233, Underhill Ctr, VT 05490 USA. EM Abby.vandenBerg@uvm.edu RI van den Berg, Abby/N-1829-2013 FU United States Department of Agriculture Cooperative State Research, Education, and Extension Service (USDA CSREES) grant [2008-34157-19186] FX This work was supported by United States Department of Agriculture Cooperative State Research, Education, and Extension Service (USDA CSREES) grant 2008-34157-19186. The authors would like to thank Ben Dana, Douglas Edwards, Marianne McKee, and Brian Stowe for their assistance with this research. NR 14 TC 2 Z9 2 U1 2 U2 23 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD AUG PY 2012 VL 114 IS 1364 BP 572 EP 576 PG 5 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA 988YN UT WOS:000307527500031 ER PT J AU Yang, JY Moon, E Nam, SH Friedman, M AF Yang, Jun Young Moon, Eunpyo Nam, Seok Hyun Friedman, Mendel TI Antidiabetic Effects of Rice Hull Smoke Extract on Glucose-Regulating Mechanism in Type 2 Diabetic Mice SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE antidiabetic effects; rice hull smoke; mice; high-fat diet; type 2 diabetes; pancreas; liver; glucose-regulating genes; glucose-regulating enzymes; cholesterol; insulin; triglycerides ID LIQUID SMOKE; INSULIN-RESISTANCE; BETA-CELL; INFLAMMATION; PROTEINS; MELLITUS; OBESITY; STRESS; TISSUE; FAT AB The aim of this study is to determine the protective effect of a liquid rice hull smoke extract (RHSE) against type 2 diabetes (T2D) in mice induced by a high-fat diet. As compared to the control group of mice on a high-fat diet (HFD), feeding the HFD supplemented with 0.5% or 1% RHSE for 7 weeks resulted in significantly reduced blood glucose and triglyceride and cholesterol concentrations, higher serum insulin levels, and improved glucose tolerance, as assessed by an oral glucose tolerance assay. The hypoglycemic effect of RESE was accompanied by changes in enzyme activities and cognate gene expression assessed using RT-PCR Among the glucose metabolism regulating genes evaluated, hepatic glucokinase (GCK), the glucose transporters GLUT2 and GLUT4, and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) were up-regulated, whereas glucose-6-phosphatase (G6 Pase) and phosphoenolpyruvate carboxykinase (PEPCK) were down-regulated in the liver of mice with RHSE-supplementation. These changes resulted in restoration of glucose-regulating activities to normal control levels. Histopathology showed that a high-fat diet intake also induced liver necrosis and damage of the islet of Langerhans in the pancreas, whereas RHSE supplementation restored necrotic damage to normal levels. Immunohistochemistry showed that RHSE supplementation can restore the reduced insulin-producing beta-cell population in islet of Langerhans associated with a high-fat diet intake to nondiabetic normal control levels in a dose-dependent manner. RISE-supplemented food could protect insulin-producing islet cells against damage triggered by oxidative stress and local inflammation associated with diabetes. C1 [Yang, Jun Young; Moon, Eunpyo; Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. EM shnam@ajou.ac.kr; mendel.friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 FU Rural Development Administration, Republic of Korea [200901OFT113068122] FX This research received grant support from the Agenda Program (No. 200901OFT113068122), Rural Development Administration, Republic of Korea. NR 38 TC 6 Z9 6 U1 1 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 1 PY 2012 VL 60 IS 30 BP 7442 EP 7449 DI 10.1021/jf3017749 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 981BX UT WOS:000306941600016 PM 22803686 ER PT J AU Vaira, AM Lim, HS Bauchan, GR Owens, RA Natilla, A Dienelt, MM Reinsel, MD Hammond, J AF Vaira, Anna Maria Lim, Hyoun-Sub Bauchan, Gary R. Owens, Robert A. Natilla, Angela Dienelt, Margaret M. Reinsel, Michael D. Hammond, John TI Lolium latent virus (Alphaflexiviridae) coat proteins: expression and functions in infected plant tissue SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID FOXTAIL MOSAIC-VIRUS; TO-CELL MOVEMENT; TRANSLATION INITIATION; MOLECULAR CHARACTERIZATION; RESTRICTION ENZYMES; TERMINAL REGION; CAPSID PROTEIN; IN-VITRO; SEQUENCE; SUPPRESSION AB The genome of Lolium latent virus (LoLV; genus Lola virus, family Alphaflexiviridae) is encapsidated by two carboxy-coterminal coat protein (CP) variants (about 28 and 33 kDa), in equimolar proportions. The CP ORF contains two 5'-proximal AUGs encoding Met 1 and Met 49, respectively promoting translation of the 33 and 28 kDa CP variants. The 33 kDa CP N-terminal domain includes a 42 aa sequence encoding a putative chloroplast transit peptide, leading to protein cleavage and alternative derivation of the approximately 28 kDa CP. Mutational analysis of the two in-frame start codons and of the putative proteolytic-cleavage site showed that the N-terminal sequence is crucial for efficient cell-to-cell movement, functional systemic movement, homologous CP interactions and particle formation, but is not required for virus replication. Blocking production of the 28 kDa CP by internal initiation shows no major outcome, whereas additional mutation to prevent proteolytic cleavage at the chloroplast membrane has a dramatic effect on virus infection. C1 [Vaira, Anna Maria; Dienelt, Margaret M.; Reinsel, Michael D.; Hammond, John] ARS, USDA, USNA, Floral & Nursery Plants Res Unit, Beltsville, MD USA. [Vaira, Anna Maria] Ist Virol Vegetale CNR, I-10135 Turin, Italy. [Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Taejon 305764, South Korea. [Bauchan, Gary R.] ARS, USDA, PSI, Electron & Confocal Microscopy Unit, Beltsville, MD USA. [Owens, Robert A.; Natilla, Angela] ARS, USDA, PSI, Mol Plant Pathol Lab, Beltsville, MD USA. RP Hammond, J (reprint author), ARS, USDA, USNA, Floral & Nursery Plants Res Unit, 10300 Baltimore Ave, Beltsville, MD USA. EM John.Hammond@ars.usda.gov RI Vaira, Anna Maria/B-9082-2015 NR 34 TC 4 Z9 4 U1 0 U2 8 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD AUG PY 2012 VL 93 BP 1814 EP 1824 DI 10.1099/vir.0.042960-0 PN 8 PG 11 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 992AD UT WOS:000307746100022 PM 22573739 ER PT J AU Yan, CG Wu, M Cao, J Tang, HF Zhu, M Johnson, PF Gao, HW AF Yan, Chunguang Wu, Min Cao, Jay Tang, Huifang Zhu, Mei Johnson, Peter F. Gao, Hongwei TI Critical Role for CCAAT/Enhancer-Binding Protein beta in Immune Complex-Induced Acute Lung Injury SO JOURNAL OF IMMUNOLOGY LA English DT Article ID NF-KAPPA-B; MONOCYTE CHEMOATTRACTANT PROTEIN-1; TRANSCRIPTION FACTORS; C/EBP-BETA; GENE-EXPRESSION; MESSENGER-RNA; MACROPHAGES; IGG; ACTIVATION; DELTA AB C/EBPs, particularly C/EBP beta and C/EBP delta, are known to participate in the regulation of many genes associated with inflammation. However, very little is known regarding the activation and functions of C/EBPb and C/EBPd in acute lung inflammation and injury. In this study, we show that both C/EBP beta and C/EBP delta activation are triggered in lungs and in alveolar macrophages following intrapulmonary deposition of IgG immune complexes. We further show that mice carrying a targeted deletion of the C/EBP beta gene displayed significant attenuation of the permeability index (lung vascular leak of albumin), lung neutrophil accumulation (myeloperoxidase activity), total number of WBCs, and neutrophils in bronchoalveolar lavage fluids compared with wildtype mice. Moreover, the mutant mice expressed considerably less TNF-alpha, IL-6, and CXC/CC chemokine and soluble ICAM-1 proteins in bronchoalveolar lavage fluids, and corresponding mRNAs in the IgG immune complex-injured lung, compared with wild-type mice. These phenotypes were associated with a significant reduction in morphological lung injury. In contrast, C/EBPd deficiency had no effect on IgG immune complex-induced lung injury. IgG immune complex-stimulated C/EBP beta-deficient alveolar macrophages released significantly less TNF-a, IL-6, MIP-2, keratinocyte cell-derived chemokine, and MIP-1 alpha compared with wild-type cells. Similar decreases in IgG immune complex-induced inflammatory mediator production were observed following small interfering RNA ablation of C/EBP beta in a murine alveolar macrophage cell line. These findings implicate C/EBPb as a critical regulator of IgG immune complex-induced inflammatory responses and injury in the lung. The Journal of Immunology, 2012, 189: 1480-1490. C1 [Yan, Chunguang; Tang, Huifang; Gao, Hongwei] Harvard Univ, Dept Anesthesiol Perioperat & Pain Med, Ctr Expt Therapeut & Reperfus Injury, Brigham & Womens Hosp,Med Sch, Boston, MA 02115 USA. [Yan, Chunguang; Wu, Min] Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58202 USA. [Cao, Jay] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Zhu, Mei] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA. [Johnson, Peter F.] NCI, Lab Canc Prevent, Ctr Canc Res, NIH, Frederick, MD 21702 USA. RP Gao, HW (reprint author), Harvard Univ, Dept Anesthesiol Perioperat & Pain Med, Ctr Expt Therapeut & Reperfus Injury, Brigham & Womens Hosp,Med Sch, 20 Shattuck St, Boston, MA 02115 USA. EM hgao@zeus.bwh.harvard.edu FU National Institutes of Health Grants [5R01HL092905-04, 3R01HL092905- 02S1]; National Institutes of Health [ES014690]; Flight Attendant Medical Research Institute [103007]; National Institutes of Health; National Cancer Institute; Center for Cancer Research; U.S. Department of Agriculture Agricultural Research Service Current Research Information System Program [5450-51000-046-00D] FX This work was supported by National Institutes of Health Grants 5R01HL092905-04 and 3R01HL092905- 02S1 (to H. G.), National Institutes of Health Grant ES014690, Flight Attendant Medical Research Institute Grant 103007 (to M. W.), the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research (to P.F.J.), and the U.S. Department of Agriculture Agricultural Research Service Current Research Information System Program Grant 5450-51000-046-00D (to J.C.). NR 43 TC 15 Z9 15 U1 0 U2 3 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 1 PY 2012 VL 189 IS 3 BP 1480 EP 1490 DI 10.4049/jimmunol.1200877 PG 11 WC Immunology SC Immunology GA 976QJ UT WOS:000306599100045 PM 22732594 ER PT J AU Armstrong, P Kelley, DS Newman, JW Staggers, FE Hartiala, J Allayee, H Stephensens, CB AF Armstrong, Patrice Kelley, Darshan S. Newman, John W. Staggers, Frank E., Sr. Hartiala, Janna Allayee, Hooman Stephensens, Charles B. TI Arachidonate 5-Lipoxygenase Gene Variants Affect Response to Fish Oil Supplementation by Healthy African Americans SO JOURNAL OF NUTRITION LA English DT Article ID N-3 FATTY-ACIDS; HIGH-DENSITY-LIPOPROTEIN; DIETARY DOCOSAHEXAENOIC ACID; CARDIOVASCULAR RISK-FACTORS; CORONARY-ARTERY-DISEASE; ALPHA-LINOLENIC ACID; BLOOD-PRESSURE; HYPERTRIGLYCERIDEMIC MEN; EICOSAPENTAENOIC ACID; MYOCARDIAL-INFARCTION AB Arachidonate 5-lipoxygenase (ALOX5) gene variants that are common in people of African ancestry are associated with a differential cardiovascular disease (CVD) risk that may be ameliorated by intake of (n-3) PUFA, such as EPA or DHA. We conducted a double-masked, placebo (PL)-controlled trial of fish oil (FO) supplements to determine if changes in erythrocyte (n-3) PUFA composition, heart rate, blood pressure, and plasma lipid and lipoprotein concentrations are modified by genotype. Participants received 5 g/d FO (2 g EPA, 1 g DHA) or 5 g/d corn/soy oil (PL). A total of 116 healthy adults of African ancestry with selected genotypes (genotypes = "dd," "d5," and "55" with "d" representing the deletion of 1 or 2 Sp1 binding sites in the ALOX5 promoter and "5" indicating the common allele with 5 sites) were enrolled and 98 completed the study. FO caused significant increases (relative to PL) in erythrocyte EPA, DHA, and total (n-3) PUPA and a decrease in the (n-6) PUFA:(n-3) PUFA ratio in the low-CVD risk "d5" and "55" genotypes but not in the high-risk "dd" genotype. Similarly, HDL particle concentration decreased with FO relative to PL in the "d5" and "55" but not "dd" genotypes. The plasma TG concentration decreased significantly with FO relative to PL in the "d5" but not "dd" and "55" genotypes. No changes were seen in LDL particle or cholesterol concentrations, heart rate, or blood pressure. These findings indicate that the efficacy of FO supplements vary by ALOX5 genotype. J. Nutr. 142: 1417-1428, 2012. C1 [Armstrong, Patrice; Kelley, Darshan S.; Newman, John W.; Stephensens, Charles B.] USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Armstrong, Patrice] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Armstrong, Patrice] Univ Calif Davis, Program Int & Community Nutr, Davis, CA 95616 USA. [Staggers, Frank E., Sr.] Alta Bates Summit Med Ctr, Ethn Hlth Inst, Oakland, CA USA. [Hartiala, Janna; Allayee, Hooman] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. RP Stephensens, CB (reprint author), USDA, Western Human Nutr Res Ctr, Davis, CA USA. EM Charles.Stephensen@ars.usda.gov FU NIH [AT003411, HL079353, P60MD0222]; USDA [5306-51530-006-00D]; Gustavus and Louise Pfeiffer Research Foundation; NIH from the National Center for Research Resources [RR10600-01, CA62528-01, RR14514-01] FX Supported by NIH grants (AT003411 to C.B.S., HL079353 to HA., and P60MD0222 to the UC Davis Center of Excellence in Nutritional Genomics) and USDA grant (CRIS project no. 5306-51530-006-00D to C.B.S.). P.A. was supported through a fellowship award from the Gustavus and Louise Pfeiffer Research Foundation. A portion of this work was conducted in a facility constructed with support from the NIH Research Facilities Improvement Program (RR10600-01, CA62528-01, RR14514-01) from the National Center for Research Resources. Reference to a company or product name does not imply approval or recommendation of the product by the USDA to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 42 TC 7 Z9 7 U1 0 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2012 VL 142 IS 8 BP 1417 EP 1428 DI 10.3945/jn.112.159814 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 980AE UT WOS:000306865200003 PM 22739369 ER PT J AU Cao, JJ Gregoire, BR Zeng, HW AF Cao, Jay J. Gregoire, Brian R. Zeng, Huawei TI Selenium Deficiency Decreases Antioxidative Capacity and Is Detrimental to Bone Microarchitecture in Mice SO JOURNAL OF NUTRITION LA English DT Article ID PHASEOLUS-VULGARIS L.; OSTEOCLAST DIFFERENTIATION; GLUTATHIONE PEROXIDASE-1; GROWTH-RETARDATION; HYDROGEN-PEROXIDE; PINTO BEANS; BODY-MASS; IN-VITRO; RATS; OSTEOPROTEGERIN AB Selenium (Se), an essential mineral, plays a major role in cellular redox status and may have beneficial effects on bone health. The objective of this study was to determine whether Se deficiency affects redox status and bone microarchitecture in a mouse model. Thirty-three male C57BL/6J mice, 18 wk old, were randomly assigned to 3 groups. Mice were fed either a purified, Se-deficient diet (SeDef) containing similar to 0.9 mu g Se/kg diet, or Se-adequate diets containing similar to 100 mu g Se/kg diet from either selenomethionine (SeMet) or pinto beans (SeBean) for 4 mo. The Se concentration, glutathione peroxidase (GPx1) activity, and GPx1 mRNA in liver were lower in the SeDef group than in the SeMet or SeBean group. The femoral trabecular bone volume/total volume and trabecular number were less, whereas trabecular separation was greater, in the SeDef group than in either the SeMet or SeBean group (P < 0.05). Bone structural parameters between the SeMet and SeBean groups did not differ. Furthermore, Serum concentrations of C-reactive protein, tartrate-resistant acid phosphatase, and intact parathyroid hormone were higher in the SeDef group than in the other 2 groups. These findings demonstrate that Se deficiency is detrimental to bone microarchitecture by increasing bone resorption, possibly through decreasing antioxidative potential. J. Nutr. 142: 1526-1531, 2012. C1 [Cao, Jay J.; Gregoire, Brian R.; Zeng, Huawei] Agr Res Serv, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. RP Cao, JJ (reprint author), Agr Res Serv, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. EM Jay.Cao@ars.usda.gov FU USDA FX Supported by the USDA Agricultural Research Service program "Bone Metabolism in Obesity." Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 46 TC 19 Z9 19 U1 0 U2 8 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD AUG PY 2012 VL 142 IS 8 BP 1526 EP 1531 DI 10.3945/jn.111.157040 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 980AE UT WOS:000306865200016 PM 22739365 ER PT J AU Moorthy, D Peter, I Scott, TM Parnell, LD Lai, CQ Crott, JW Ordovas, JM Selhub, J Griffith, J Rosenberg, IH Tucker, KL Troen, AM AF Moorthy, Denish Peter, Inga Scott, Tammy M. Parnell, Laurence D. Lai, Chao-Qiang Crott, Jimmy W. Ordovas, Jose M. Selhub, Jacob Griffith, John Rosenberg, Irwin H. Tucker, Katherine L. Troen, Aron M. TI Status of Vitamins B-12 and B-6 but Not of Folate, Homocysteine, and the Methylenetetrahydrofolate Reductase C677T Polymorphism Are Associated with Impaired Cognition and Depression in Adults SO JOURNAL OF NUTRITION LA English DT Article ID CONTROLLED-TRIAL; PLASMA FOLATE; MTHFR GENE; FOLIC-ACID; METHIONINE SYNTHASE; ALZHEIMERS-DISEASE; VASCULAR-DISEASE; GENOME-WIDE; RISK-FACTOR; LATER LIFE AB The C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene differs in frequency in various ethnic groups that have differing prevalence of age-related cognitive impairments. We used a series of neuro-psychological tests to examine the association of the MTHFR C677T polymorphism with cognition and depression and also to assess whether genotype modifies the association of folate and homocysteine with these outcomes. This study analyzed pooled cross-sectional data from 2 ethnically diverse cohorts of community-living adults: the Boston Puerto Rican Health Study (n = 939) and the Nutrition, Aging, and Memory in Elders study (n = 1017). Individuals in both cohorts underwent anthropometric and laboratory measurements and dietary and health assessments using validated questionnaires between the years 2003 and 2007. Cognitive outcomes included measures of global cognition [Mini-Mental Status Exam (MMSE)], depression (Center for Epidemiological Studies Depression Scale), and 3 factor scores for the domains of attention, executive function, and memory that were derived from a detailed set of neuropsychological tests. Low plasma vitamin B-12 concentrations were associated with poorer MMSE scores and higher depression scores, and low vitamin B-6 concentrations were associated with lower MMSE and worse attention and executive function in the multivariate analysis. In contrast, MTHFR genotype, folate, and homocysteine were not associated with cognition or depression in either ethnicity-pooled or stratified analysis. The current study did not find evidence of an association between the MTHFR C677T TT genotype and impaired cognition or depression in a population with adequate folate status and a high prevalence of cognitive impairment and depression. J. Nutr. 142: 1554-1560, 2012. C1 [Moorthy, Denish; Scott, Tammy M.; Rosenberg, Irwin H.; Troen, Aron M.] Tufts Univ, Neurosci & Aging Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Parnell, Laurence D.; Lai, Chao-Qiang; Ordovas, Jose M.] Tufts Univ, Nutr Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Crott, Jimmy W.] Tufts Univ, Vitamins & Carcinogenesis Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Selhub, Jacob] Tufts Univ, Vitamin Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Peter, Inga] Mt Sinai Sch Med, Dept Genet & Genom Sci, New York, NY USA. [Scott, Tammy M.] Tufts Med Ctr, Dept Psychiat, Boston, MA USA. [Griffith, John] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Boston, MA USA. [Troen, Aron M.] Hebrew Univ Jerusalem, Inst Biochem Food Sci & Nutr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel. RP Troen, AM (reprint author), Tufts Univ, Neurosci & Aging Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM DMoorthy@tuftsmedicalcenter.org; Troen@agri.huji.ac.il OI Tucker, Katherine/0000-0001-7640-662X FU Ross Institute of Aging, NIH [POI AG023394]; NIA; NIH [MO1-RR00054]; USDA [58-1950-9-001]; [RO1 AG21790] FX Supported by a pilot grant to AMT from the Ross Institute of Aging, NIH POI AG023394, NIA; RO1 AG21790; NIH MO1-RR00054 and USDA (under cooperative agreement no. 58-1950-9-001). NR 61 TC 27 Z9 27 U1 0 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2012 VL 142 IS 8 BP 1554 EP 1560 DI 10.3945/jn.112.161828 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 980AE UT WOS:000306865200020 PM 22739363 ER PT J AU Ren, ZH Na, LX Xu, YM Rozati, M Wang, JP Xu, JG Sun, CH Vidal, K Wu, DY Meydani, SN AF Ren, Zhihong Na, Lixin Xu, Yanmei Rozati, Mitra Wang, Junpeng Xu, Jianguo Sun, Changhao Vidal, Karine Wu, Dayong Meydani, Simin Nikbin TI Dietary Supplementation with Lacto-Wolfberry Enhances the Immune Response and Reduces Pathogenesis to Influenza Infection in Mice SO JOURNAL OF NUTRITION LA English DT Article ID LYCIUM-BARBARUM POLYSACCHARIDES; VITAMIN-E SUPPLEMENTATION; A VIRUS-INFECTION; AGED MICE; LIQUID-CHROMATOGRAPHY; LUNG IMMUNOPATHOLOGY; OXIDATIVE STRESS; PROTEIN COMPLEX; C57BL/6 MICE; CELLS AB Despite the availability of vaccines, influenza is a considerable public health problem, which emphasizes the need for development of additional strategies to enhance host defense against influenza. Wolfberry, or goji berry, long used as a medicinal food in China, has recently been shown to improve immune response in mice. Because immune response plays a key role in the body's defense against pathogens, we hypothesized that wolf berry may increase host resistance to influenza infection by enhancing immune response. To test this hypothesis, we fed adult mice (4 mo old) a milk-based preparation of wolfberry called Lacto-Wolfberry (LWB) for 4 wk and then infected them with influenza A/Puerto Rico/8/34 (HI NI) while continuing the same experimental diets. Viral titer, lung pathology, and immune response were determined at different time points postinfection. LWB supplementation prevented infection-induced weight loss and reduced lung pathology on days 6 and 9 postinfection (P < 0.05). LWB-fed mice showed overall, significantly higher concanavalin A-induced IL-2 production (P < 0.05). Furthermore, we found positive correlations between weight loss and lung viral titer, pathology score, TNF alpha, and IL-6 production as well as negative correlations with T cell proliferation and IL-2 production (all P <= 0.05). These results indicate that LWB supplementation can attenuate symptoms and pathology of influenza infection by decreasing inflammatory cytokines in lungs while enhancing systemic T cell-mediated function as measured by their ability to produce IL-2. J. Nutr. 142: 1596-1602, 2012. C1 [Ren, Zhihong; Na, Lixin; Xu, Yanmei; Rozati, Mitra; Wang, Junpeng; Wu, Dayong; Meydani, Simin Nikbin] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. [Ren, Zhihong; Xu, Yanmei; Xu, Jianguo] China CDC, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China. [Na, Lixin; Sun, Changhao] Harbin Med Univ, Coll Publ Hlth, Dept Food Hyg & Nutr, Harbin, Peoples R China. [Vidal, Karine] Nestle Res Ctr, Nutr & Hlth Dept, CH-1000 Lausanne, Switzerland. RP Meydani, SN (reprint author), Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. EM dayong.wu@tufts.edu; simin.meydani@tufts.edu FU Nestec Ltd.; USDA/ARS [58-1950-7-707] FX Supported, in part, by a research grant from Nestec Ltd. and by the USDA/ARS under contract no. 58-1950-7-707. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 52 TC 6 Z9 6 U1 1 U2 18 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2012 VL 142 IS 8 BP 1596 EP 1602 DI 10.3945/jn.112.159467 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 980AE UT WOS:000306865200026 PM 22739381 ER PT J AU Slisz, AM Breksa, AP Mishchuk, DO McCollum, G Slupsky, CM AF Slisz, Anne M. Breksa, Andrew P., III Mishchuk, Darya O. McCollum, Greg Slupsky, Carolyn M. TI Metabolomic Analysis of Citrus Infection by 'Candidatus Liberibacter' Reveals Insight into Pathogenicity SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE citrus; Citrus sinensis; Huanglongbing; metabolomics; NMR; Valencia oranges ID RECOVERED GRAPEFRUIT SEEDLINGS; PROTEIN AMINO ACIDS; REAL-TIME PCR; QUANTITATIVE CHANGES; FRUIT; SPECTROSCOPY; HEALTHY; DISEASE; LEAVES; JUICE AB Huanglongbing (HLB), considered the most serious citrus disease in the world, is associated with the nonculturable bacterium 'Candidatus Liberibacter asiaticus' (Las). Infection of citrus by this pathogen leads to reduced plant vigor and productivity, ultimately resulting in death of the infected tree. It can take up to two years following initial infection before outward symptoms become apparent, making detection difficult. The existing knowledge gap in our understanding of Las and its pathogenesis leading to HLB has stymied development of treatments and methods to mitigate the pathogen's influence. To evaluate the influence of Las on fruit quality in both symptomatic and asymptomatic fruit, and gain further insight into the pathogenesis of the disease, a H-1 NMR metabolomics investigation, complemented with physicochemical and analyte-specific analyses, was undertaken. Comparison of the juice obtained from oranges gathered from Last (symptomatic and asymptomatic) and Las(-) (healthy) trees revealed significant differences in the concentrations of sugars, amino and organic acids, limonin glucoside, and limonin. This study demonstrates differing metabolic profiles in the juice of oranges from Las(+) and Las(-) and proposes how Las may be able to evade citrus defense responses. C1 [Slupsky, Carolyn M.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Slisz, Anne M.; Mishchuk, Darya O.; Slupsky, Carolyn M.] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Breksa, Andrew P., III] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [McCollum, Greg] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Slupsky, CM (reprint author), Univ Calif Davis, Dept Nutr, 1 Shields Ave, Davis, CA 95616 USA. EM cslupsky@ucdavis.edu FU Citrus Research Board; California Department of Food and Agriculture [SCB08507]; USDA, ARS CRIS Project [5325-41430-011-00D, 6618-21000-013-00D] FX The authors are indebted to Drs. Mark Hi If and Aifric O'Sullivan for critical reading of the manuscript, and Dr. Mark Hill for helpful suggestions. The authors gratefully acknowledge funding for this project from the Citrus Research Board and the California Department of Food and Agriculture (Grant No. SCB08507). This study was supported in part by the USDA, ARS CRIS Project No. 5325-41430-011-00D and Project No. 6618-21000-013-00D. NR 37 TC 20 Z9 20 U1 1 U2 51 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 J9 J PROTEOME RES JI J. Proteome Res. PD AUG PY 2012 VL 11 IS 8 BP 4223 EP 4230 DI 10.1021/pr300350x PG 8 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 982IW UT WOS:000307037600027 PM 22698301 ER PT J AU Morris, MS Selhub, J Jacques, PF AF Morris, Martha Savaria Selhub, Jacob Jacques, Paul F. TI Vitamin B-12 and Folate Status in Relation to Decline in Scores on the Mini-Mental State Examination in the Framingham Heart Study SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE aged; cognition; folic acid; humans; methylmalonic acid; vitamin B 12 ID FOLIC-ACID FORTIFICATION; LOW SERUM VITAMIN-B12; COGNITIVE IMPAIRMENT; METHYLMALONIC ACID; COBALAMIN DEFICIENCY; CELIAC-DISEASE; OLDER-ADULTS; BIOCHEMICAL INDICATORS; GERIATRIC OUTPATIENTS; ELDERLY POPULATION AB Objectives To investigate the cognitive significance of low to low-normal plasma vitamin B-12 concentrations and to shed light on the role that folate status plays in the association between vitamin B-12 status and cognitive decline. Design Associations between plasma vitamin B-12 and folate and 8-year cognitive decline were evaluated, and the effects of interactions between vitamin B-12 status and folate status and supplemental folate use on cognitive decline were assessed. Setting The Framingham Heart Study a prospective epidemiological study. Participants Five hundred forty-nine community-dwelling individuals aged 74.8 +/- 4.6. Measurements Mini-Mental State Examination (MMSE), plasma folate, vitamin B-12, methylmalonic acid, homocysteine, demographic factors, and body mass index. Results MMSE scores declined by 0.24 points per year over the 8-year follow-up period. Decline was significantly faster in cohort members in the bottom two plasma vitamin B-12 quintile categories, and no apparent cognitive advantage was associated with plasma vitamin B-12 from 187 to 256.8 pmol/L over less than 186 pmol/L. In cohort members with plasma vitamin B-12 less than 258 pmol/L, having a plasma folate concentration greater than 20.2 nmol/L was associated with an approximate 1-point per year decline, as was use of supplemental folate. Conclusion Plasma vitamin B-12 levels from 187 to 256.8 pmol/L predict cognitive decline. Furthermore, having plasma vitamin B-12 levels in this range or below in conjunction with high plasma folate or supplemental folate use predicts especially rapid cognitive decline. C1 [Morris, Martha Savaria; Selhub, Jacob; Jacques, Paul F.] Tufts Univ, Nutr Epidemiol Program, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Morris, MS (reprint author), Tufts Univ, Nutr Epidemiol Program, Jean Mayer USDA, Human Nutr Res Ctr Aging, 711 Washington St,Room 901D, Boston, MA 02111 USA. EM martha.morris@tufts.edu FU U.S. Department of Agriculture [58-1950-7-707]; National Institutes of Health [1 R01 NS062877-01A2] FX Funded by U.S. Department of Agriculture Agreement 58-1950-7-707 and National Institutes of Health Grant 1 R01 NS062877-01A2. NR 55 TC 34 Z9 35 U1 3 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0002-8614 EI 1532-5415 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD AUG PY 2012 VL 60 IS 8 BP 1457 EP 1464 DI 10.1111/j.1532-5415.2012.04076.x PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 989EE UT WOS:000307542300009 PM 22788704 ER PT J AU Dorr, BS Burger, LW Barras, SC Godwin, KC AF Dorr, Brian S. Burger, Loren W. Barras, Scott C. Godwin, Kristina Casscles TI Economic Impact of Double-Crested Cormorant, Phalacrocorax auritus, Depredation on Channel Catfish, Ictalurus punctatus, Aquaculture in Mississippi, USA SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID DELTA REGION; FINGERLINGS; INDUSTRY; PONDS AB The Yazoo River Basin of Mississippi, USA, supports the largest concentration of hectares devoted to channel catfish, Ictalurus punctatus, aquaculture production in North America. The Yazoo Basin also supports large numbers of resident, wintering and migrating fish-eating birds, with the Double-crested Cormorant, Phalacrocorax auritus, implicated as the most serious depredating species. We used data from aerial surveys of numbers and distribution of cormorants in the Yazoo Basin and on commercial catfish ponds during winters (NovemberApril) 20002001 and 20032004 to refine estimates of regional economic losses due to cormorant depredation. In both periods, the greatest monthly estimates of cormorant foraging occurred from 1 January to 31 March. Losses in terms of biomass, number, and dollar value were greater for foodfish ponds than fingerling ponds. Monthly weighted estimates of catfish consumed were 1775.3 and 1346.6 m.t. over winters 20002001 and 20032004, respectively. Total estimated losses for foodfish and fingerling ponds in 20002001 were $11.56 and $0.48 million, respectively, and in 20032004 were $5.22 and $0.40 million, respectively. Maximum dollar loss occurred during March in 20002001 and during February in 20032004. In this study, the volatility in variable production costs and nominal sales price, and distribution of cormorants on pond types and regionally were key factors in resulting economic loss estimates. C1 [Dorr, Brian S.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, Mississippi State, MS 39762 USA. [Burger, Loren W.] Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Barras, Scott C.] Wildlife Serv, USDA, Moseley, VA 23120 USA. RP Dorr, BS (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. OI Dorr, Brian/0000-0001-6857-8560 FU USDA/WS/National Wildlife Research Center [QA-829] FX USDA/WS/National Wildlife Research Center provided funding to conduct this research under Quality Assurance protocol QA-829. We thank staff with United States Department of Agriculture, Wildlife Services, Mississippi, for aerial night roost surveys, particularly G. Ellis and S. Swafford and A. "Butch" Nygren for his professional aerial survey service. We also thank T. Hanson, K. Hanson-Dorr, A. Prisock, F. Cunningham, J. Glahn and two anonymous reviewers for their comments and editorial assistance. NR 38 TC 4 Z9 4 U1 0 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD AUG PY 2012 VL 43 IS 4 BP 502 EP 513 DI 10.1111/j.1749-7345.2012.00586.x PG 12 WC Fisheries SC Fisheries GA 982OG UT WOS:000307054500005 ER PT J AU Chatakondi, NG Torrans, EL AF Chatakondi, Nagaraj G. Torrans, Eugene L. TI The Influence of Ovarian Fluid pH of Stripped Unfertilized Channel Catfish, Ictalurus punctatus, Eggs on the Hatching Success of Channel Catfish female x Blue Catfish, Ictalurus furcatus male, Hybrid Catfish Eggs SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; BIOCHEMICAL PARAMETERS; INDUCED OVULATION; SALMO-TRUTTA; QUALITY; FRY AB This study was designed to determine the effect of ovarian fluid pH of stripped unfertilized channel catfish, Ictalurus punctatus, eggs on fertilization and hatch rate of channel catfish ? x blue catfish, Ictalurus furcatus?, hybrid catfish eggs. A significant correlation was established between ovarian fluid pH of stripped channel catfish eggs and hybrid embryo hatch rate (R2 = 0.75, P = 0.01), suggesting ovarian fluid pH of stripped catfish eggs prior to fertilization can be predictive of the hatching success of hybrid catfish embryos. These data were used to categorize pH of stripped eggs: pH <7.0 as low pH eggs, pH 7.07.4 as medium pH eggs, or pH >7.4 as high pH eggs quality eggs. The range in percent hatch rate for these pH categories was <15%, 1530%, and >30%, respectively. Higher calcium concentrations during incubation do not appear to improve hatching success of low pH eggs. The predictive model presented herein describes a quick method for evaluating stripped catfish eggs for hybrid fry production in catfish hatcheries. C1 [Chatakondi, Nagaraj G.; Torrans, Eugene L.] USDA ARS, Catfish Genet Res Unit, Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. RP Chatakondi, NG (reprint author), USDA ARS, Catfish Genet Res Unit, Natl Warmwater Aquaculture Ctr, POB 38, Stoneville, MS 38776 USA. NR 23 TC 3 Z9 3 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD AUG PY 2012 VL 43 IS 4 BP 585 EP 593 DI 10.1111/j.1749-7345.2012.00577.x PG 9 WC Fisheries SC Fisheries GA 982OG UT WOS:000307054500013 ER PT J AU Durso, LM Wells, JE Harhay, GP Rice, WC Kuehn, L Bono, JL Shackelford, S Wheeler, T Smith, TPL AF Durso, L. M. Wells, J. E. Harhay, G. P. Rice, W. C. Kuehn, L. Bono, J. L. Shackelford, S. Wheeler, T. Smith, T. P. L. TI Comparison of bacterial communities in faeces of beef cattle fed diets containing corn and wet distillers' grain with solubles SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE diversity; ecology; Escherichia coli; intestinal microbiology; veterinary ID GUT MICROBIOME; GEN. NOV.; PREVALENCE; DIVERSITY; DATABASE; HIDES; LEVEL AB Aim: The mammalian intestinal microflora has been shown to impact host physiology. In cattle, intestinal bacteria are also associated with faecal contamination of environmental sources and human illness via foodborne pathogens. Use of wet distillers grains with solubles (WDGS) in cattle feed creates a gastrointestinal environment where some bacterial species are enriched. Here, we examine if a diet containing 40% WDGS results in fundamentally different microbial community structures. Methods and Results: The 20 002 16S r-RNA gene sequences from 20 beef cattle were analysed using Sanger sequencing methods. At the genus level, Prevotella (Gram negative) and Anaerobacter (Gram positive) were the most frequently occurring bacteria in our beef cattle faecal samples. Diet-associated differences in prevalence were noted for Prevotella but not Anaerobacter. Conclusions: Diet affects community structure. Faecal communities of co-housed beef cattle are not identical. Significance and Impact of the Study: It is known that a diet of 40% corn-based WDGS increases the generic Escherichia coli in the faeces and enriches E. coli O157:H7. The results from the current study suggest that in addition to previously observed changes in E. coli, the entire bacterial community structure is different for animals fed 40% corn-based WDGS compared to a traditional corn-finishing diet. C1 [Durso, L. M.] ARS, USDA, AMRU, Lincoln, NE 68583 USA. [Wells, J. E.; Harhay, G. P.; Kuehn, L.; Bono, J. L.; Shackelford, S.; Wheeler, T.; Smith, T. P. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Rice, W. C.] ARS, USDA, Renewable Energy & Manure Management Res Unit, Bushland, TX USA. RP Durso, LM (reprint author), ARS, USDA, AMRU, Room 137,Keim Hall,UNL E Campus, Lincoln, NE 68583 USA. EM lisa.durso@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service, National Program [108] FX We thank Ron Mlejnek, Steve Simcox, Bob Lee, Sandy Fryda-Bradley and Jaime LaBrie for technical assistance; Donna Griess for secretarial assistance; Randy Bradley, Phil Anderson, William Dailey and Myron Coleman for IT support; and the MARC cattle crew for assistance with sample collection. This research was supported by the US Department of Agriculture, Agricultural Research Service, National Program 108. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 18 TC 15 Z9 16 U1 2 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD AUG PY 2012 VL 55 IS 2 BP 109 EP 114 DI 10.1111/j.1472-765X.2012.03265.x PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 978IY UT WOS:000306735200003 PM 22583366 ER PT J AU Watts, DB Arriaga, FJ Torbert, HA Busby, RR Gebhart, DL AF Watts, Dexter B. Arriaga, Francisco J. Torbert, H. Allen Busby, Ryan R. Gebhart, Dick L. TI Noncomposted Municipal Solid Waste Byproduct Influences Soil and Plant Nutrients 5 Years After Soil Reclamation SO SOIL SCIENCE LA English DT Article DE Municipal solid waste; soil restoration; plant nutrients ID COMPOST; CARBON; TILLAGE AB Concerns for the mounting supply of municipal solid waste being generated combined with decreasing landfill space have compelled military installations to evaluate alternative methods for disposal. One approach to reduce landfilling is the use of a new garbage-processing technology that sterilizes and separates the waste into inorganic and organic components. Thus, a study was initiated to evaluate the effectiveness of using the organic component (Fluff) as a soil amendment for reclamation of disturbed US Army training land. The Fluff material was initially incorporated (10-20 cm) into a highly degraded sandy loam soil (fine-loamy, kaolinitic, thermic Typic Kandiudults) located in a borrow pit at Fort Benning Military Reservation, Georgia, in 2003. The Fluff was applied at rates of 0, 18, 36, 72, and 143 Mg ha(-1), and the soil was seeded with native prairie grasses. Soil nutrient retention and plant uptake were evaluated to determine the soil-plant system's sustainability after 5 years. An unseeded control was also evaluated as a comparison of natural recovery. Five years after reclamation, vegetation resulting from natural recovery in the unseeded control was sparse. Fluff addition increased pH, organic matter, and plant nutrient availability in the degraded soil, with the greatest improvements occurring at higher application rates. Soil productivity improvements resulted in greater plant biomass production. Generally, plant nutrient concentrations were not significantly impacted by Fluff addition. However, plant nutrient content was consistently higher with Fluff addition, which was attributed to increased biomass production. Plant nutrient uptakes of N, Ca, Mg, B, Mn, and Zn were within the reference range for grasses, whereas P and K concentrations were slightly deficient. An Fe and Cu concentration toxicity problem at the borrow site was alleviated with higher Fluff application rates, whereas the control and lower Fluff rates accumulated high levels of Fe and Cu within plant tissues. These results suggest that Fluff can be effectively used in land rehabilitation and revegetation practices to create a sustainable native grassland ecosystem. C1 [Watts, Dexter B.; Arriaga, Francisco J.; Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Busby, Ryan R.; Gebhart, Dick L.] USA, Construct Engn Res Lab, Ecol Proc Branch, Engn Res & Dev Ctr, Champaign, IL 61824 USA. RP Watts, DB (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM Dexter.Watts@ars.usda.gov NR 34 TC 0 Z9 0 U1 2 U2 23 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X J9 SOIL SCI JI Soil Sci. PD AUG PY 2012 VL 177 IS 8 BP 480 EP 489 DI 10.1097/SS.0b013e318268a246 PG 10 WC Soil Science SC Agriculture GA 989HL UT WOS:000307550800002 ER PT J AU Haywood, JD AF Haywood, James D. TI Pine Straw Harvesting, Fire, and Fertilization Affect Understory Vegetation within a Louisiana Longleaf Pine Stand SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE diammonium phosphate; Pinus palustris Mill.; potash; triple superphosphate; understory plant community AB Pine straw harvesting can provide an economic benefit to landowners, but the practice may also change the composition of plant communities. This research was initiated in a 34-year-old stand of longleaf pine (Pinus palustris Mill.) established in 1956 to study how pine straw management practices (fertilization, prescribed fire, and straw harvesting) affected plant communities, and herein, effects on understory vegetation are reported. A randomized complete block split-plot design was installed with two main plots: (1) no fertilization and (2) fertilization three times with different combinations of N, P, and K. There were four subplot treatments: (1) control, (2) prescribed fire, (3) prescribed fire and 2 harvests of pine straw, and (4) 13 annual harvests of pine straw. Fertilization significantly increased grass cover as a percentage of surface area. However, there was a general lack of understory plant response to nutrient amendments, partly because pine straw harvesting and burning were confounding fertilization effects. Prescribed fire and mechanical harvesting activities created similar understories on subplot treatments 2, 3, and 4 by significantly reducing understory woody plant stature and removing litter. Increases in understory tree and shrub stature, number of woody vines per acre, and percentage of woody plant cover significantly decreased herbaceous plant yields and percentage of cover. C1 US Forest Serv, So Res Stn, Alexandria Forestry Ctr, Pineville, LA 71360 USA. RP Haywood, JD (reprint author), US Forest Serv, So Res Stn, Alexandria Forestry Ctr, 2500 Shreveport Highway, Pineville, LA 71360 USA. EM dhaywood@fs.fed.us NR 27 TC 1 Z9 1 U1 2 U2 30 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2012 VL 36 IS 3 BP 130 EP 135 DI 10.5849/sjaf.10-054 PG 6 WC Forestry SC Forestry GA 990HU UT WOS:000307622400004 ER PT J AU Kaetzel, BR Majumdar, I Teeter, LD Butler, BJ AF Kaetzel, Brandon R. Majumdar, Indrajit Teeter, Larry D. Butler, Brett J. TI Regional Differences among Family Forest Landowners Using National Woodland Owner Survey Results SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE nonindustrial private forest landowners; National Woodland Owner Survey ID NORTHERN CUMBERLAND PLATEAU; MOTIVATIONS; OBJECTIVES; EDUCATION; TENNESSEE; SERVICES; BEHAVIOR; STATES AB Family forest landowners have traditionally been considered fairly homogeneous. A number of new studies in the past decade, however, have demonstrated that landowners and their reasons for owning forestland differ substantially. These landowners own approximately 35% of our nation's forestland, and they warrant further research concerning these differences. So far, there has been no substantial examination of regional differences among family forest owners considering landowner characteristics. Individuals own land for a variety of reasons (aesthetics, income, etc.), and many external considerations may affect how they manage (such as proximity to neighboring lands and mimicking neighboring land management regimes). National Woodland Owner Survey results are used along with previously assigned motivation clusters regarding why family forest owners own their woodland in this article. This study examined landowner characteristics along with the existing literature and attempted to weave an explanation regarding regional differences among family forest owners. Results indicate that there are differences among family forest owners from region to region, and these can be explained in the context of the literature. The results could be useful in the future as resource professionals attempt to target appropriate information to landowners concerning sustainable forest management. C1 [Kaetzel, Brandon R.; Teeter, Larry D.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Butler, Brett J.] US Forest Serv, Amherst, MA 01003 USA. RP Kaetzel, BR (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 3301 Forestry & Wildlife Sci Bldg, Auburn, AL 36849 USA. EM brk0002@tigermail.auburn.edu NR 36 TC 2 Z9 2 U1 2 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2012 VL 36 IS 3 BP 141 EP 145 DI 10.5849/sjaf.11-007 PG 5 WC Forestry SC Forestry GA 990HU UT WOS:000307622400006 ER PT J AU Haywood, JD Sung, SJS Sayer, MAS AF Haywood, James D. Sung, Shi-Jean Susana Sayer, Mary Anne Sword TI Copper Root Pruning and Container Cavity Size Influence Longleaf Pine Growth through Five Growing Seasons SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE Copperblocks; copper oxychloride; Pinus palustris Mill.; regeneration; Superblocks ID PRESCRIBED FIRE; HEIGHT GROWTH; SEEDLINGS; ESTABLISHMENT; FERTILIZATION; HERBICIDES AB Restoring longleaf pine (Pinus palustris Mill.) over much of its historic range requires artificial regeneration, and most often, container-grown seedlings are used. However, type and size of container can influence field performance. In this study, longleaf pine seedlings were grown in Beaver Plastics Styroblocks either without a copper treatment (Superb lock) or with a copper oxychloride coating (Copperblock) and with three sizes of cavities that were 60, 108, and 164 ml. Seedlings from the six container types (two types of Styroblocks with three cavity sizes) were planted in central Louisiana in a 2 by 3 randomized complete block factorial design. Emergence from the grass stage was quickest for seedlings outplanted from either Copperblocks or large cavities (164 ml), but 99.3% of all seedlings had emerged after five growing seasons. Five-year-old trees outplanted from Copperblocks were significantly taller and had greater volume index (VI = [groundline diameter](2) x [total height]) than trees outplanted from Superb locks (2.0 m tall and 114 VI versus 1.7 m tall and 87 VI). Trees outplanted from small cavities (60 ml) were shorter and had a smaller VI (1.5 m tall and 73 VI) than trees outplanted from the other two cavity sizes (average of 2.0 m tall and 114 VI). C1 [Haywood, James D.; Sung, Shi-Jean Susana; Sayer, Mary Anne Sword] US Forest Serv, So Res Stn, Pineville, LA 71360 USA. RP Haywood, JD (reprint author), US Forest Serv, So Res Stn, 2500 Shreveport Highway, Pineville, LA 71360 USA. EM dhaywood@fs.fed.us NR 23 TC 3 Z9 3 U1 2 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2012 VL 36 IS 3 BP 146 EP 151 DI 10.5849/sjaf.10-051 PG 6 WC Forestry SC Forestry GA 990HU UT WOS:000307622400007 ER PT J AU Vadala, CE Bixler, RD Bransford, JJ Waldrop, TA AF Vadala, Carin E. Bixler, Robert D. Bransford, Jeffrey J. Waldrop, Thomas A. TI Attitudes, Knowledge, and Perception of Fuel Reduction among Involved Publics in the Southern Appalachians: Implications for Responsive Communication SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE southern Appalachian Mountains; prescribed fire; mechanical fuel reduction; communication strategies; knowledge; attitudes ID TOPIC INTEREST; PARTICIPATION; STRATEGIES; MANAGEMENT; FIRE AB An understanding of how identifiable groups perceive fuel reduction will help forest managers develop formal and informal communication strategies responsive to each group's concerns. This study identified three opinion segments on the basis of their attitudinal and behavioral characteristics about fuel reduction in the southern Appalachians and further described them on the basis of general forest use attitudes and behaviors In = 640). A Let Nature Take Its Course segment was preservation oriented and supported fuel reduction only to the extent that it was thought to enhance biological diversity. More trusting of federal land managers, they should be approached through natural history and hiking clubs with messages emphasizing effects on nongame species. A Management for Human Benefits group was most supportive of fuel reduction and was concerned about availability of game and nongame species. More trusting of state land managers and strongly supportive of fuel reduction, they should be approached with messages about human benefits of fuel reduction. A Visually Appealing segment tended to evaluate fuel reduction mostly on the basis of aesthetic impacts. This amorphous group is more likely to object to fuel reduction on the basis of aesthetic issues, such as charring, downed timber, and loss of rhododendron and mountain laurel. Each group should be approached through a different channel, by forest managers from agencies it most prefers, with message content emphasizing perspectives on forest management already salient to the group. C1 [Vadala, Carin E.] Natl Pk Serv, Anchorage, AK 99501 USA. [Bixler, Robert D.] Clemson Univ, Dept Pk Recreat & Tourism Management, Clemson, SC 29634 USA. [Bransford, Jeffrey J.] Indiana Univ, Eppley Inst Pk & Publ Lands, Bloomington, IN 47404 USA. [Waldrop, Thomas A.] Clemson Univ, US Forest Serv, So Res Stn, Clemson, SC 29634 USA. RP Vadala, CE (reprint author), Natl Pk Serv, 240 W 5th Ave, Anchorage, AK 99501 USA. EM rbixler@clemson.edu FU US Forest Service, Southern Research Station, Center for Forest Disturbance Science [SRS-4156] FX Carin E. Vadala, National Park Service, 240 W 5th Ave., Anchorage, AK 99501. Robert D. Bixler, Department of Parks, Recreation and Tourism Management, Clemson University, 275A Lehotsky Hall, Clemson, SC 29634. Jeffrey J. Bransford, Eppley Institute for Parks & Public Lands, Indiana University, 501 N Morton, Suite 101, Bloomington, IN 47404. Thomas A. Waldrop, US Forest Service, Southern Research Station, 239 Lehotsky Hall, Clemson University, Clemson, SC 29634. Copies of the questionnaire or additional information may be obtained from Robert D. Bixler (rbixler@clemson.edu). Funding for this work was provided by the US Forest Service, Southern Research Station, Center for Forest Disturbance Science, SRS-4156. NR 18 TC 1 Z9 1 U1 1 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2012 VL 36 IS 3 BP 159 EP 165 DI 10.5849/sjaf.08-029 PG 7 WC Forestry SC Forestry GA 990HU UT WOS:000307622400009 ER PT J AU Ghazvini, H Hiebert, CW Zegeye, T Liu, SX Dilawari, M Tsilo, T Anderson, JA Rouse, MN Jin, Y Fetch, T AF Ghazvini, Habibollah Hiebert, Colin W. Zegeye, Taye Liu, Sixin Dilawari, Mridull Tsilo, Toi Anderson, James A. Rouse, Matthew N. Jin, Yue Fetch, Tom TI Inheritance of resistance to Ug99 stem rust in wheat cultivar Norin 40 and genetic mapping of Sr42 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; COMMON BUNT RESISTANCE; PUCCINIA-GRAMINIS; AC CADILLAC; RACE TTKS; VIRULENCE; MARKERS; BARLEY; SR31; MAP AB Stem rust, caused by Puccinia graminis f. sp. tritici, is a devastating disease of wheat. The emergence of race TTKSK (Ug99) and new variants in Africa threatens wheat production worldwide. The best method of controlling stem rust is to deploy effective resistance genes in wheat cultivars. Few stem rust resistance (Sr) genes derived from the primary gene pool of wheat confer resistance to TTKSK. Norin 40, which carries Sr42, is resistant to TTKSK and variants TTKST and TTTSK. The goal of this study was to elucidate the inheritance of resistance to Ug99 in Norin 40 and map the Sr gene(s). A doubled haploid (DH) population of LMPG-6/Norin 40 was evaluated for resistance to the race TTKST. Segregation of 248 DH lines fitted a 1:1 ratio (chi (2) 1:1= 0.58, p = 0.45), indicating a single gene in Norin 40 conditioned resistance to Ug99. This was confirmed by an independent F-2:3 population also derived from the cross LMPG-6/Norin 40 where a 1:2:1 ratio (chi (2)1:2:1 = 0.69, p = 0.71) was observed following the inoculation with race TTKSK. Mapping with DNA markers located this gene to chromosome 6DS, the known location of Sr42. PCR marker FSD_RSA co-segregated with Sr42, and simple sequence repeat (SSR) marker BARC183 was closely linked (0.5 cM) to Sr42. A previous study found close linkage between FSD_RSA and SrCad, a temporarily designated gene that also confers resistance to Ug99, thus Sr42 may be the same gene or allelic. Marker FSD_RSA is suitable for marker-assisted selection (MAS) in wheat breeding programs to improve stem rust resistance, including Ug99. C1 [Ghazvini, Habibollah; Hiebert, Colin W.; Zegeye, Taye; Fetch, Tom] Agr & Agri Food Canada, Cereal Res Ctr, Winnipeg, MB R3T 2M9, Canada. [Liu, Sixin; Dilawari, Mridull; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Tsilo, Toi] Small Grain Inst, Agr Res Council, Bethlehem, South Africa. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Hiebert, CW (reprint author), Agr & Agri Food Canada, Cereal Res Ctr, 195 Dafoe Rd, Winnipeg, MB R3T 2M9, Canada. EM colin.hiebert@agr.gc.ca RI Rouse, Matthew/G-5474-2011; Tsilo, Toi/G-5088-2011; OI Ghazvini, Habibollah/0000-0002-0840-5585 NR 32 TC 26 Z9 26 U1 0 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2012 VL 125 IS 4 BP 817 EP 824 DI 10.1007/s00122-012-1874-y PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 985TY UT WOS:000307294600016 PM 22580967 ER PT J AU Bosch, DD Truman, CC Potter, TL West, LT Strickland, TC Hubbard, RK AF Bosch, David D. Truman, Clint C. Potter, Thomas L. West, Larry T. Strickland, Timothy C. Hubbard, Robert K. TI Tillage and slope position impact on field-scale in the South Atlantic Coastal Plain SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Conservation tillage; Runoff; Subsurface flow; Infiltration ID SOIL ORGANIC-MATTER; CONSERVATION-TILLAGE; SURFACE RUNOFF; PHOSPHORUS TRANSPORT; SIMULATED RAINFALL; MANURE APPLICATION; UNITED-STATES; CROP RESIDUE; WATER LOSSES; ERODED SOIL AB There is widespread interest in increasing conservation tillage use during row crop production. However, long-term hydrologic data are needed to better evaluate the potential benefits of conservation tillage. To that end, hydrologic monitoring was conducted for 11 years on a 1.2 ha hillslope located in the Atlantic Coastal Plain region of south central Georgia, USA. The site was equally divided between two tillage treatments, conventional-tillage (CT) and strip-tillage (ST). with cotton and peanut produced in rotation. Each tillage block was divided into three 0.2 ha plots to evaluate runoff as a function of tillage and slope position. Soil texture and hydraulic characteristic data were quantified to evaluate potential impacts on hydrologic processes including runoff, infiltration, and lateral subsurface flow. Surface runoff from CT averaged 22% of annual precipitation while that from ST averaged 13%. When paired by tillage and landscape position, annual runoff from ST was significantly less than annual runoff from CT (p=0.025). Monthly surface runoff from CT was consistently greater than that from ST. particularly during May through August. Increased infiltration as a consequence of the reduced tillage led to significantly greater average annual subsurface losses from ST(19%) than from CT (10%) (p = 0.025). Results indicated that slope position and textural differences may also have influenced site hydrology. Plots at the top of the landscape where finer soil textures were observed produced greater runoff than plots at lower landscape positions where coarser textures were observed. In summary, the ST cotton/peanut rotation can be expected to significantly reduce runoff but increase lateral subsurface flow. As a consequence, water budgets from the two tillage systems were essentially equal. Similar decreases in surface runoff and increases in lateral subsurface flow can be expected to occur in landscapes in the Atlantic Coastal Plain Region where strip tillage is adopted. Published by Elsevier B.V. C1 [Bosch, David D.; Truman, Clint C.; Potter, Thomas L.; Strickland, Timothy C.; Hubbard, Robert K.] USDA ARS SEWRL, Tifton, GA 31794 USA. [West, Larry T.] USDA NRCS Natl Soil Survey Ctr, Lincoln, NE USA. RP Bosch, DD (reprint author), USDA ARS SEWRL, POB 748, Tifton, GA 31794 USA. EM david.bosch@ars.usda.gov; clint.truman@ars.usda.gov; tom.potter@ars.usda.gov; larry.west@lin.usda.gov; tim.strickland@ars.usda.gov; robert.hubbard@ars.usda.gov FU U.S. Department of Agriculture; University of Georgia; Georgia Cotton Commission; U.S.-Israel Binational Agricultural Research and Development Fund FX This research was made possible through the support of the U.S. Department of Agriculture, the University of Georgia, the Georgia Cotton Commission and the U.S.-Israel Binational Agricultural Research and Development Fund. Technical assistance of Ernest Harris, Laura Marshall, Ricky Fletcher, Margie Whittle, Sally Belflower, Lorine Lewis, and many undergraduate students is greatly appreciated. NR 48 TC 11 Z9 12 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 J9 AGR WATER MANAGE JI Agric. Water Manage. PD AUG PY 2012 VL 111 BP 40 EP 52 DI 10.1016/j.agwat.2012.05.002 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 982FU UT WOS:000307029100006 ER PT J AU Novotny, JA Gebauer, SK Baer, DJ AF Novotny, Janet A. Gebauer, Sarah K. Baer, David J. TI Discrepancy between the Atwater factor predicted and empirically measured energy values of almonds in human diets SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID CORONARY-HEART-DISEASE; NUT CONSUMPTION; LIPID BIOACCESSIBILITY; METABOLIZABLE ENERGY; CONVERSION FACTORS; BODY-WEIGHT; RISK; HEALTH; WOMEN; SEEDS AB Background: The energy content of foods is primarily determined by the Atwater factors, which may not be accurate for certain food groups. Nuts are a food group for which substantial evidence suggests that the Atwater factors may be poorly predictive. Objective: A study was conducted to determine the energy value of almonds in the human diet and to compare the measured energy value with the value calculated from the Atwater factors. Design: Eighteen healthy adults consumed a controlled diet or an almond-containing diet for 18 d. Three treatments were administered to subjects in a crossover design, and diets contained 1 of 3 almond doses: 0, 42, or 84 g/d. During the final 9 d of the treatment period, volunteers collected all urine and feces, and samples of diets, feces, and urine were analyzed for macronutrient and energy contents. The metabolizable energy content of the almonds was determined. Results: The energy content of almonds in the human diet was found to be 4.6 +/- 0.8 kcal/g, which is equivalent to 129 kcal/28-g serving. This is significantly less than the energy density of 6.0-6.1 kcal/g as determined by the Atwater factors, which is equivalent to an energy content of 168-170 kcal/serving. The Atwater factors, when applied to almonds, resulted in a 32% overestimation of their measured energy content. Conclusion: This study provides evidence for the inaccuracies of the Atwater factors for certain applications and provides a rigorous method for determining empirically the energy value of individual foods within the context of a mixed diet. This trial was registered at clinicaltrials.gov as NCT01007188. Am J Clin Nutr 2012;96:296-301. C1 [Novotny, Janet A.; Gebauer, Sarah K.; Baer, David J.] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. RP Novotny, JA (reprint author), ARS, USDA, Beltsville Human Nutr Res Ctr, 307B Ctr Rd, Beltsville, MD 20705 USA. EM janet.novotny@ars.usda.gov FU USDA; Almond Board of California FX Supported by the USDA and the Almond Board of California. NR 28 TC 45 Z9 45 U1 2 U2 25 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD AUG PY 2012 VL 96 IS 2 BP 296 EP 301 DI 10.3945/ajcn.112.035782 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 978TW UT WOS:000306769700012 PM 22760558 ER PT J AU Green, BT Welch, KD Gardner, DR Stegelmeier, BL Pfister, JA Cook, D Panter, KE AF Green, Benedict T. Welch, Kevin D. Gardner, Dale R. Stegelmeier, Bryan L. Pfister, James A. Cook, Daniel Panter, Kip E. TI Toxicokinetics of norditerpenoid alkaloids from low larkspur (Delphinium andersonii) orally administered to cattle SO AMERICAN JOURNAL OF VETERINARY RESEARCH LA English DT Article ID NUTTALLIANUM; METHYLLYCACONITINE; BARBEYI; CONSUMPTION; TOXICITY; IDAHO; SPP.; MICE AB Objective-To determine the toxicokinetics of N-(methylsuccinimido)anthranoyllycoctonine-type low larkspur alkaloids in beef cattle. Animals-5 Black Angus steers and 35 Swiss Webster mice. Procedures-Low larkspur (Delphinium andersonii) was collected, dried, ground, and administered to 5 steers via oral gavage to provide a dose of 12 mg of N-(methylsuccinimido)-anthranoyllycoctonine alkaloids/kg. Steers were housed in metabolism crates for 96 hours following larkspur administration; heart rate was monitored continuously, and blood samples were collected periodically for analysis of serum concentrations of 16-deacetylgeyerline, methyllycaconitine, geyerline, and nudicauline and assessment of kinetic parameters. The LD50 of a total alkaloid extract from D andersonii was determined in Swiss Webster mice. Results-The alkaloids were quickly absorbed, with a maximum serum concentration achieved within 18 hours after administration. Geyerline and nudicauline coeluted as 1 peak and were considered together for toxicokinetic analysis. Mean +/- SD elimination half-life was 18.4 +/- 4.4 hours, 15.6 +/- 1.5 hours, and 16.5 +/- 5.1 hours for 16-deacetylgeyerline, methyllycaconitine, and geyerline and nudicauline, respectively. There were significant differences in maximum serum concentration, amount absorbed, and distribution half-life among the 4 alkaloids. The mouse LD50 was 9.8 mg/kg. Conclusions and Clinical Relevance-Results suggested that clinical poisoning was likely to be most severe approximately 18 hours after exposure. Cattle should be closely monitored for at least 36 hours after initial exposure. Additionally, a withdrawal time of approximately 7 days would be required to clear > 99% of the toxic alkaloids from the serum of cattle that have ingested low larkspur. (Am J Vet Res 2012;73:1318-1324) C1 [Green, Benedict T.; Welch, Kevin D.; Gardner, Dale R.; Stegelmeier, Bryan L.; Pfister, James A.; Cook, Daniel; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84321 USA. RP Green, BT (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84321 USA. EM Ben.Green@ars.usda.gov FU USDA Agricultural Research Service FX Supported by the USDA Agricultural Research Service. NR 22 TC 5 Z9 5 U1 1 U2 7 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0002-9645 J9 AM J VET RES JI Am. J. Vet. Res. PD AUG PY 2012 VL 73 IS 8 BP 1318 EP 1324 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA 981PN UT WOS:000306981700024 PM 22849693 ER PT J AU Pike, KS Graf, G Foottit, RG Maw, HEL von Dohlen, C Harpel, J Pantoja, A Emmert, S Hagerty, AM AF Pike, K. S. Graf, G. Foottit, R. G. Maw, H. E. L. von Dohlen, C. Harpel, J. Pantoja, A. Emmert, S. Hagerty, A. M. TI Eriosomatine aphids (Hemiptera: Aphididae: Eriosomatinae) associated with moss and roots of conifer and willow in forests of the Pacific Northwest of North America SO CANADIAN ENTOMOLOGIST LA English DT Article ID GALL APHID; LIFE-CYCLE; PEMPHIGINAE AB Apterous adult morphs of eriosomatine aphids associated with moss (Bryophyta) and/or roots of conifer (Pinaceae) or willow (Salix Linnaeus (Salicaceae)) in forests of the North American Pacific Northwest including Alaska are described, illustrated, and keyed. In total, seven species (Clydesmithia canadensis Danielsson, Melaphis rhois (Fitch) (moss only feeder), Pachypappa rosettei (Maxson), Pachypappa sacculi (Gillette), Prociphilus americanus (Walker) (fir root only feeder), Prociphilus xylostei (De Geer), and Thecabius populimonilis (Riley)) are characterised from their secondary host habitats. Secondary host forms of C. canadensis and T. populimonilis are described for the first time. The morphotypes from the secondary hosts were confirmed through deoxyribonucleic acid sequence matching with those from the primary hosts. C1 [Pike, K. S.; Graf, G.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Foottit, R. G.; Maw, H. E. L.] Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, Canada. [von Dohlen, C.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Harpel, J.] Univ British Columbia, UBC Herbarium, Vancouver, BC V6T 1Z4, Canada. [Pantoja, A.] Reg Off Latin Amer & Caribbean, United Nations Food & Agr Org, Santiago, Chile. [Emmert, S.] USDA APHIS PPQ, Madison, WI 53726 USA. [Hagerty, A. M.] Arizona Cotton Res Ctr, Casa Grande, AZ 85193 USA. [Pantoja, A.; Emmert, S.; Hagerty, A. M.] Univ Alaska Fairbanks, Subarct Agr Res Unit, ARS, USDA, Fairbanks, AK 99775 USA. RP Pike, KS (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM kpike@wsu.edu FU Genome Canada (through the Ontario Genomics Institute); Natural Sciences and Engineering Research Council of Canada FX The authors express thanks to D. Graf, (Washington State University technical staff), D. Fleming, B. Kunibe, S. Lillard, R. Pampell, and R. Ranft (United States Department of Agriculture-Agricultural Research Services, Alaska); M. Bowser (Kenai National Wildlife Refuge, Soldotna, Alaska), and L. Pike (Prosser, Washington) for assistance in field collecting and Berlese extraction; and D. Graf for specimen clearing and mounting. For the loan of specimens or making collections available for study they thank R. L. Blinn, North Carolina State University, Raleigh, North Carolina; J. Pitts, Utah State University, Logan, Utah; G. Miller, United States Department of Agriculture, Systematic Entomology Laboratory, Beltsville, Maryland; and the Canadian National Collection of Insects, Arachnids and Nematodes (Agriculture and Agri-Food Canada, Ottawa, Ontario). The authors also thank the United States National Forest Service for permission to sample, and J. Frelich and G. Hunter for facilitating collections in the Olympic National Park. DNA sequencing was supported through funding to the Canadian Barcode of Life Network from Genome Canada (through the Ontario Genomics Institute), Natural Sciences and Engineering Research Council of Canada and other sponsors listed at www.BOLNET.ca. NR 41 TC 2 Z9 2 U1 1 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD AUG PY 2012 VL 144 IS 4 BP 555 EP 576 DI 10.4039/tce.2012.49 PG 22 WC Entomology SC Entomology GA 984ZS UT WOS:000307233100005 ER PT J AU Lyashchenko, KP Greenwald, R Esfandiari, J Mikota, S Miller, M Moller, T Vogelnest, L Gairhe, KP Robbe-Austerman, S Gai, J Waters, WR AF Lyashchenko, Konstantin P. Greenwald, Rena Esfandiari, Javan Mikota, Susan Miller, Michele Moller, Torsten Vogelnest, Larry Gairhe, Kamal P. Robbe-Austerman, Suelee Gai, Jackie Waters, W. Ray TI Field Application of Serodiagnostics To Identify Elephants with Tuberculosis prior to Case Confirmation by Culture SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID CAPTIVE ASIAN ELEPHANTS; STAT-PAK ASSAY; MYCOBACTERIUM-TUBERCULOSIS; RAPID DETECTION; ANTIBODY-RESPONSES; INFECTION; BOVIS; TESTS; DIAGNOSIS; DISEASE AB Three serologic methods for antibody detection in elephant tuberculosis (TB), the multiantigen print immunoassay (MAPIA), ElephantTB STAT-PAK kit, and DPP VetTB test, were evaluated using serial serum samples from 14 captive elephants infected with Mycobacterium tuberculosis in 5 countries. In all cases, serological testing was performed prior to the diagnosis of TB by mycobacterial culture of trunk wash or tissue samples collected at necropsy. All elephants produced antibody responses to M. tuberculosis antigens, with 13/14 recognizing ESAT-6 and/or CFP10 proteins. The findings supported the high serodiagnostic test accuracy in detecting infections months to years before M. tuberculosis could be isolated from elephants. The MAPIA and/or DPP VetTB assay demonstrated the potential for monitoring antimycobacterial therapy and predicting TB relapse in treated elephants when continuously used in the posttreatment period. History of exposure to TB and past treatment information should be taken into consideration for proper interpretation of the antibody test results. Data suggest that the more frequent trunk wash culture testing of seropositive elephants may enhance the efficiency of the TB diagnostic algorithm, leading to earlier treatment with improved outcomes. C1 [Lyashchenko, Konstantin P.; Greenwald, Rena; Esfandiari, Javan] Chembio Diagnost Syst Inc, Medford, NY USA. [Mikota, Susan] Elephant Care Int, Hohenwald, TN USA. [Mikota, Susan] Elephant Sanctuary Tennessee, Hohenwald, TN USA. [Miller, Michele] Palm Beach Zoo, Palm Beach, FL USA. [Moller, Torsten] Kolmarden Zoo & Wildlife Pk, Kolmarden, Sweden. [Vogelnest, Larry] Taronga Zoo, Mosman, Australia. [Robbe-Austerman, Suelee] USDA, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, Ames, IA 50010 USA. [Gai, Jackie] Zoo & Exot Anim Consultat, Vacaville, CA USA. [Waters, W. Ray] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Lyashchenko, KP (reprint author), Chembio Diagnost Syst Inc, Medford, NY USA. EM klyashchenko@chembio.com OI Miller, Michele/0000-0002-5883-6076 FU U.S. Fish and Wildlife Service; Asian Elephant Conservation Fund FX Gratitude is extended to the Department of National Parks and Wildlife Conservation, the National Trust for Nature Conservation, the World Wildlife Fund-Nepal, and Sarad Paudel, Jeewan Thapa, and Barbara Vincent for TB work in Nepal as well as to the U.S. Fish and Wildlife Service, Asian Elephant Conservation Fund, for financial support. NR 29 TC 12 Z9 12 U1 0 U2 18 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD AUG PY 2012 VL 19 IS 8 BP 1269 EP 1275 DI 10.1128/CVI.00163-12 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 983HR UT WOS:000307110900021 PM 22695162 ER PT J AU Lehotay, SJ Lightfield, AR Geis-Asteggiante, L Schneider, MJ Dutko, T Ng, C Bluhm, L Mastovska, K AF Lehotay, Steven J. Lightfield, Alan R. Geis-Asteggiante, Lucia Schneider, Marilyn J. Dutko, Terry Ng, Chilton Bluhm, Louis Mastovska, Katerina TI Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra-high performance liquid chromatography - tandem mass spectrometry SO DRUG TESTING AND ANALYSIS LA English DT Article DE veterinary drugs residues analysis; UHPLC-MS; MS; bovine kidney; method validation; qualitative screening ID MULTIRESIDUE ANALYSIS; ANTIBIOTIC-RESIDUES; LC-MS/MS; FOOD; EXTRACTION; MATRICES; SERUM; JUICE; MEAT AB In the USA, the US Department of Agriculture's Food Safety and Inspection Service (FSIS) conducts the National Residue Program designed to monitor veterinary drug and other chemical residues in beef and other slaughtered food animals. Currently, FSIS uses a 7-plate bioassay in the laboratory to screen for antimicrobial drugs in bovine kidneys from those animals tested positive by inspectors in the slaughter establishments. The microbial inhibition bioassay has several limitations in terms of monitoring scope, sensitivity, selectivity, and analysis time. Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) has many advantages over the bioassay for this application, and this study was designed to develop, evaluate, and validate a fast UHPLC-MS/MS method for antibiotics and other high-priority veterinary drugs in bovine kidney. Five existing multi-class, multi-residue methods from the literature were tested and compared, and each performed similarly. Experiments with incurred samples demonstrated that a 5-min shake of 2?g homogenized kidney with 10?ml of 4/1 (v/v) acetonitrile/water followed by simultaneous clean-up of the initial extract with 0.5?g C18 and 10?ml hexane gave a fast, simple, and effective sample preparation method for the <10?min UHPLC-MS/MS analysis. An extensive 5-day validation process demonstrated that the final method could be used to acceptably screen for 54 of the 62 drugs tested, and 50 of those met qualitative MS identification criteria. Quantification was not needed in the application, but the method gave =70% recoveries and =25% reproducibilities for 30 of the drugs. Published 2012. This article is a U.S. Government work and is in the public domain of the USA. C1 [Lehotay, Steven J.; Lightfield, Alan R.; Geis-Asteggiante, Lucia; Schneider, Marilyn J.; Mastovska, Katerina] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Geis-Asteggiante, Lucia] UdelaR, Fac Quim, Catedra Farmacognosia & Prod Nat, DQO, Montevideo, Uruguay. [Dutko, Terry] Food Safety & Inspect Serv, USDA, Midwestern Lab, St Louis, MO USA. [Ng, Chilton; Bluhm, Louis] US Food Safety & Inspect Serv, USDA, Lab Qual Assurance Div, St Louis, MO USA. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov FU Agricultural Research Service [60-1935-9-031]; Food Safety Inspection Service [0-1935-9-031]; US-Israel Binational Agricultural Research and Development Fund [US-4273-09]; US Department of Agriculture FX This work was conducted under Interagency Agreement No. #60-1935-9-031 between the Agricultural Research Service and the Food Safety Inspection Service. This work was also supported by the US-Israel Binational Agricultural Research and Development Fund grant US-4273-09. The authors thank Biotage for the ENV+ that sinks in MeCN, Perry Martos of the University of Guelph for helpful discussions, and Waters Corp. for help with use of the instrumentation.; This project was directly funded by the US Department of Agriculture. There are no other conflicts of interest. NR 31 TC 22 Z9 22 U1 1 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1942-7603 J9 DRUG TEST ANAL JI Drug Test. Anal. PD AUG PY 2012 VL 4 SU 1 BP 75 EP 90 DI 10.1002/dta.1363 PG 16 WC Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy GA 981HG UT WOS:000306957000011 PM 22851364 ER PT J AU Schneider, MJ Lehotay, SJ Lightfield, AR AF Schneider, Marilyn J. Lehotay, Steven J. Lightfield, Alan R. TI Evaluation of a multi-class, multi-residue liquid chromatography - tandem mass spectrometry method for analysis of 120 veterinary drugs in bovine kidney SO DRUG TESTING AND ANALYSIS LA English DT Article DE multi-class veterinary drug residues analysis; LC-MS; MS; MS identification; bovine kidney ID ANTIBIOTICS; VALIDATION; MUSCLE AB Traditionally, regulatory monitoring of veterinary drug residues in food animal tissues involves the use of several single-class methods to cover a wide analytical scope. Multi-class, multi-residue methods (MMMs) of analysis tend to provide greater overall laboratory efficiency than the use of multiple methods, and liquid chromatographytandem mass spectrometry (LC-MS/MS) of targeted drug analytes usually provides exceptional performance even for complicated sample extracts. In this work, an LC-MS/MS method was optimized and validated in a test of 120 drug analytes from 11 different classes in bovine kidney. The method used 10 ml of 4/1 acetonitrile/water for extraction of 2 g samples and cleanup with hexane partitioning. Quantitative and qualitative performance was assessed for the analytes at fortification levels of 10, 50, 100, and 200 ng/g. With the method, 66 drugs gave 70120% recovery with =20% RSD at all levels over the course of 3 days. At the 200 ng/g level, 89 drugs met these same standards. Limits of detection were =10 ng/g for 109 of the analytes in the kidney matrix in validation experiments. Qualitatively, MS/MS identification criteria were set that ion ratios occur within +/- 10% (absolute value) from those of the analyte reference standards. At the 10 ng/g level, 57% of the drugs met the identification criteria, which improved to 84% at the 200 ng/g level. The method serves as an efficient and useful additional option among the current monitoring methods available. Published 2012. This article is a U.S. Government work and is in the public domain of the USA. C1 [Schneider, Marilyn J.; Lehotay, Steven J.; Lightfield, Alan R.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Schneider, MJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM marilyn.schneider@ars.usda.gov NR 15 TC 16 Z9 20 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1942-7603 J9 DRUG TEST ANAL JI Drug Test. Anal. PD AUG PY 2012 VL 4 SU 1 BP 91 EP 102 DI 10.1002/dta.1359 PG 12 WC Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy GA 981HG UT WOS:000306957000012 PM 22851365 ER PT J AU Wadhwa, A Bannantine, JP Byrem, TM Stein, TL Saxton, AM Speer, CA Eda, S AF Wadhwa, Ashutosh Bannantine, John P. Byrem, Todd M. Stein, Traci L. Saxton, Arnold M. Speer, C. A. Eda, Shigetoshi TI Optimization of Serum EVELISA for Milk Testing of Johne's Disease SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID AVIUM SUBSP PARATUBERCULOSIS; LINKED-IMMUNOSORBENT-ASSAY; DAIRY-CATTLE; MYCOBACTERIUM-PARATUBERCULOSIS; BOVINE-PARATUBERCULOSIS; FECAL-PCR; DIAGNOSIS; ELISA; ANTIBODIES; CULTURE AB Johne's disease (JD) or paratuberculosis, caused by Mycobacterium avium ssp. paratuberculosis (MAP), is one of the most economically important diseases of dairy cattle. Control of JD could be achieved by good herd management practices, and diagnosis; however, this approach has been hampered by the low sensitivity of currently available enzyme-linked immunosorbent assay (ELISA) tests. In our previous study, we developed a sensitive serum ELISA test, ethanol-vortex enzyme-linked immunosorbent assay (EVELISA), using ethanol extract of MAP. The objective of this study is to demonstrate that the EVELISA can be used for detection of anti-MAP antibodies in milk samples. In this study, we tested and optimized concentrations of antigen, milk, and secondary antibody for better differentiation of milk samples of cattle with MAP infections from those of cattle in JD-free herds. We evaluated five environmental mycobacteria as absorbents of cross-reactive antibodies in milk and found that the mycobacteria had no significant effect on EVELISA results. Using the optimized conditions, a total of 57 milk samples from Holstein dairy cattle (37 animals found positive on the fecal polymerase chain reaction test and 20 animals from JD-free herds) were tested for anti-MAP antibody in milk by using the EVELISA method. The average of ELISA values in the JD-positive milk samples (mean +/- SD = 0.355 +/- 0.455) was significantly higher than that in the JD-negative milk samples (mean +/- SD = 0.071 +/- 0.011). These results warrant further studies for evaluation and validation of the EVELISA for milk testing of cattle for JD. C1 [Wadhwa, Ashutosh; Speer, C. A.; Eda, Shigetoshi] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Ctr Wildlife Hlth, Knoxville, TN 37996 USA. [Saxton, Arnold M.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. [Bannantine, John P.] USDA, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Byrem, Todd M.; Stein, Traci L.] Antel Biosyst, Lansing, MI USA. RP Eda, S (reprint author), Univ Tennessee, Dept Forestry Wildlife & Fisheries, Ctr Wildlife Hlth, 274 Ellington Plant Sci Bldg, Knoxville, TN 37996 USA. EM seda@utk.edu OI Bannantine, John/0000-0002-5692-7898 FU USDA/NRI [2007-3504-1862]; Johne's Disease Integrated Program [JDIP 2008-55620-18710]; AgResearch Extension Innovation Fund of the University of Tennessee Institute of Agriculture FX The work was supported by the following grants to S.E.: USDA/NRI 2007-3504-1862, Johne's Disease Integrated Program JDIP 2008-55620-18710, and AgResearch Extension Innovation Fund of the University of Tennessee Institute of Agriculture. NR 31 TC 7 Z9 7 U1 1 U2 6 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD AUG PY 2012 VL 9 IS 8 BP 749 EP 754 DI 10.1089/fpd.2011.1115 PG 6 WC Food Science & Technology SC Food Science & Technology GA 988QU UT WOS:000307506400011 PM 22845627 ER PT J AU Schuldes, J Orbegoso, MR Schmeisser, C Krishnan, HB Daniel, R Streit, WR AF Schuldes, Jorg Orbegoso, Mariita Rodriguez Schmeisser, Christel Krishnan, Hari B. Daniel, Rolf Streit, Wolfgang R. TI Complete Genome Sequence of the Broad-Host-Range Strain Sinorhizobium fredii USDA257 SO JOURNAL OF BACTERIOLOGY LA English DT Article ID NGR234 AB Here we announce the complete genome sequence of the symbiotic and nitrogen-fixing bacterium Sinorhizobium fredii USDA257. The genome shares a high degree of sequence similarity with the closely related broad-host-range strains S. fredii NGR234 and HH103. Most strikingly, the USDA257 genome encodes a wealth of secretory systems. C1 [Schuldes, Jorg; Daniel, Rolf] Univ Gottingen, Gottingen Genom Lab, Gottingen, Germany. [Schuldes, Jorg; Daniel, Rolf] Univ Gottingen, Dept Genom & Appl Microbiol, Gottingen, Germany. [Orbegoso, Mariita Rodriguez; Schmeisser, Christel; Streit, Wolfgang R.] Univ Hamburg, Biozentrum Klein Flottbek, Abt Mikrobiol & Biotechnol, Hamburg, Germany. [Daniel, Rolf] Univ Gottingen, Inst Microbiol & Genet, Gottingen, Germany. [Krishnan, Hari B.] Agr Res Serv, Plant Genet Res Unit, USDA, Columbia, MO USA. RP Streit, WR (reprint author), Univ Gottingen, Gottingen Genom Lab, Gottingen, Germany. EM wolfgang.streit@uni-hamburg.de OI Daniel, Rolf/0000-0002-8646-7925 FU GenoMik-Plus initiative of the German Federal Ministry of Education and Research (Bundesministerium fur Bildung und Forschung [BMBF] FX This work was funded by the GenoMik-Plus initiative of the German Federal Ministry of Education and Research (Bundesministerium fur Bildung und Forschung [BMBF]). NR 6 TC 21 Z9 22 U1 0 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD AUG PY 2012 VL 194 IS 16 BP 4483 EP 4483 DI 10.1128/JB.00966-12 PG 1 WC Microbiology SC Microbiology GA 984NQ UT WOS:000307198100062 PM 22843606 ER PT J AU Torkzaban, S Wan, JM Tokunaga, TK Bradford, SA AF Torkzaban, Saeed Wan, Jiamin Tokunaga, Tetsu K. Bradford, Scott A. TI Impacts of bridging complexation on the transport of surface-modified nanoparticles in saturated sand SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Nanoparticles; Transport; Deposition; Bridging complexation; DLVO theory ID MODEL POROUS-MEDIA; FULLERENE NANOPARTICLES; QUARTZ SANDS; DEPOSITION; AGGREGATION; KINETICS; ADSORPTION; DETACHMENT; PARTICLES; STABILITY AB The transport of polyacrylic acid capped cadmium telluride (CdTe) quantum dots (QDs), carboxylate-modified latex (CML), and bare silica nanoparticles (NPs) was studied in packed columns at various electrolyte concentrations and cation types. The breakthrough curves (BTCs) of QDs and CML particles in acid-treated Accusand showed significant amounts of increasing deposition with 0.5, 1, and 2 mM Ca2+, but only minute deposition at 50 and 100 mM Na+. Negligible QD and CML deposition occurred at 2 mM Ca2+ in columns packed with ultrapure quartz sand that was similar in size to the Accusand. These observations are not consistent with interpretations based on Derjaguin-Landau-Verwey-Overbeek (DLVO) calculations of interaction energies. Scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis demonstrated that there were regions on the acid-treated Accusand covered with small amounts of clay that were absent on the ultrapure quartz sand. A salt cleaning method was therefore used to remove the clay from the acid-treated Accusand. The BTCs of QDs and CML in this acid + salt treated Accusand exhibited much less deposition at any given Ca2+ concentration compared to those obtained from the acid-treated sand. SEM images showed that most of the QD deposited in acid-treated Accusand occurred on clay surfaces. Unlike our results with QDs and CML, negligible deposition of bare silica NPs occurred at 5 and 10 mM Ca2+ in acid-treated Accusand. The high deposition of QDs and CML particles was therefore attributed to bridging complexation in which Ca2+ serves as a bridge between the cation exchange locations on the clay and carboxyl functional groups on the QD and CML particles, which were absent on the bare silica NPs. Our results suggest that the transport of carboxylic ligand-modified NPs may be limited in subsurface environments because of the ubiquitous presence of clay and divalent cations. (C) 2012 Elsevier B.V. All rights reserved. C1 [Torkzaban, Saeed] CSIRO, Glen Osmond, SA 5064, Australia. [Wan, Jiamin; Tokunaga, Tetsu K.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA. [Bradford, Scott A.] ARS, USDA, US Salin Lab, Riverside, CA USA. RP Torkzaban, S (reprint author), CSIRO, Private Bag 2, Glen Osmond, SA 5064, Australia. EM saeed.torkzaban@csiro.au RI Torkzaban, Saeed/G-7377-2013; Tokunaga, Tetsu/H-2790-2014; Wan, Jiamin/H-6656-2014 OI Torkzaban, Saeed/0000-0002-5146-9461; Tokunaga, Tetsu/0000-0003-0861-6128; FU BER-EPA-NSF Nanoparticulate Research Program of the Office of Biological and Environmental Research, U.S. Department of Energy [DE-AC02-05CH11231] FX Funding was provided through the joint BER-EPA-NSF Nanoparticulate Research Program of the Office of Biological and Environmental Research, U.S. Department of Energy, under contract DE-AC02-05CH11231. The authors are grateful to thoughtful comments and suggestions of Drs. Benjamin Gilbert and Yongman Kim from Lawrence Berkeley National Laboratory. Dr. Martin Mulvihill from the University of California, Berkeley is thanked for providing the SEM analyses. The authors would like to thank the anonymous reviewers for their valuable comments and suggestions to improve the paper. NR 37 TC 29 Z9 29 U1 3 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD AUG PY 2012 VL 136 BP 86 EP 95 DI 10.1016/j.jconhyd.2012.05.004 PG 10 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA 987OD UT WOS:000307426000007 PM 22698948 ER PT J AU Jin, T Gurtler, JB AF Jin, T. Gurtler, J. B. TI Inactivation of Salmonella on Tomato Stem Scars by Edible Chitosan and Organic Acid Coatings SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; LISTERIA-MONOCYTOGENES; SENSORY QUALITY; STORAGE-TEMPERATURE; HYDROGEN-PEROXIDE; FRESH PRODUCE; RAW TOMATOES; LACTIC-ACID; SURVIVAL; CHLORINE AB This study was conducted to investigate the efficacy of antimicrobial coatings for inactivation of Salmonella on the surface of tomato stem scars. Scars were inoculated with a four-strain cocktail of Salmonella (serovars Montevideo, Newport, Saintpaul, and Typhimurium) and coated with acid-chitosan solutions. The chitosan coating with three acids (3A plus chitosan), the chitosan coating with one acid, and the three-acid solution without chitosan reduced the populations of Salmonella by 6.0, 3.6, and 5.3 log CFU per stem scar, respectively. Addition of allyl isothiocyanate (10 mu l/ml) to the 3A plus chitosan coating did not significantly increase (P > 0.05) the antimicrobial efficacy. Although the populations of Salmonella in the controls (ca. 7.5 log CFU per stem scar) did not change significantly throughout the 14-day storage period at 10 degrees C, Salmonella cells were reduced to undetectable levels (<0.7 log CFU per stem scar) in the samples treated with 3A plus chitosan coating after two days of storage, and no growth was observed for the remaining storage period. Results from this study demonstrate that coatings of acid plus chitosan provide an alternative antimicrobial intervention for decontamination of tomatoes. C1 [Jin, T.] ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Gurtler, J. B.] ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Jin, T (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 FU U.S. Department of Agriculture, Agricultural Research Service (ARS) Current Research Information System project through ARS National Program 108 [1935-41420-092-00D] FX This study was funded by the U.S. Department of Agriculture, Agricultural Research Service (ARS) Current Research Information System project 1935-41420-092-00D through ARS National Program 108. The authors thank Anita Parameswaran and Rebecca Rivera for their excellent technical laboratory assistance. NR 34 TC 13 Z9 13 U1 0 U2 14 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2012 VL 75 IS 8 BP 1368 EP 1372 DI 10.4315/0362-028X.JFP-12-054 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 987JM UT WOS:000307413100001 PM 22856559 ER PT J AU Hwang, CA Huang, LH Sheen, S Juneja, V AF Hwang, Cheng-An Huang, Lihan Sheen, Shiowshuh Juneja, Vijay TI Effects of Lactic Acid on the Growth Characteristics of Listeria monocytogenes on Cooked Ham Surfaces SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; BEEF CARCASS SURFACES; TO-EAT MEAT; ORGANIC-ACIDS; SALMONELLA-TYPHIMURIUM; PEROXYACETIC ACID; VACUUM PACKAGES; TURKEY BREAST; PRODUCTS; DECONTAMINATION AB The surfaces of ready-to-eat meats are susceptible to postprocessing contamination by Listeria monocyto genes. This study examined and modeled the growth characteristics of L. monocyto genes on cooked ham treated with lactic acid solutions (LA). Cooked ham was inoculated with L. monocytogenes (ca. 10(3) CFU/g), immersed in 0, 0.5, 0.75, 1.0, 1.25, 1.5, and 2.0% LA for 30 min, vacuum packaged, and stored at 4, 8, 12, and 16 degrees C. LA immersion resulted in <0.7 log CFU/g immediate reduction of L. monocytogenes on ham surfaces, indicating the immersion alone was not sufficient for reducing L. monocyto genes. During storage, no growth of L. monocytogenes occurred on ham treated with 1.5% LA at 4 and 8 degrees C and with 2% LA at all storage temperatures. LA treatments extended the lag-phase duration (LPD) of L. monocytogenes and reduced the growth rate (GR) from 0.21 log CFU/day in untreated ham to 0.13 to 0.06 log CFU/day on ham treated with 0.5 to 1.25% LA at 4 degrees C, whereas the GR was reduced from 0.57 log CFU/day to 0.40 to 0.12 log CFU/day at 8 degrees C. A significant extension of the LPD and reduction of the GR of L. monocytogenes occurred on ham treated with >1.25% LA. The LPD and GR as a function of LA concentration and storage temperature can be satisfactorily described by a polynomial or expanded square-root model. Results from this study indicate that immersion treatments with >1.5% LA for 30 min may be used to control the growth of L. monocytogenes on cooked meat, and the models would be useful for selecting LA immersion treatments for meat products to achieve desired product safety. C1 [Hwang, Cheng-An; Huang, Lihan; Juneja, Vijay] ARS, USDA, Eastern Reg Res Ctr, Residue Chem & Predict Microbiol Res Unit, Wyndmoor, PA 19038 USA. [Sheen, Shiowshuh] ARS, USDA, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, Wyndmoor, PA 19038 USA. RP Hwang, CA (reprint author), ARS, USDA, Eastern Reg Res Ctr, Residue Chem & Predict Microbiol Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM andy.hwang@ars.usda.gov NR 37 TC 1 Z9 1 U1 5 U2 19 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2012 VL 75 IS 8 BP 1404 EP 1410 DI 10.4315/0362-028X.JFP-11-412 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 987JM UT WOS:000307413100005 PM 22856563 ER PT J AU Wang, R Bono, JL Kalchayanand, N Shackelford, S Harhay, DM AF Wang, Rong Bono, James L. Kalchayanand, Norasak Shackelford, Steven Harhay, Dayna M. TI Biofilm Formation by Shiga Toxin-Producing Escherichia coli O157:H7 and Non-O157 Strains and Their Tolerance to Sanitizers Commonly Used in the Food Processing Environment SO JOURNAL OF FOOD PROTECTION LA English DT Article ID MULTIPLEX PCR ASSAYS; MONOCLONAL-ANTIBODIES; CURLI EXPRESSION; STAINLESS-STEEL; O157H7 STRAINS; UNITED-STATES; SURFACES; O157-H7; O111; RESISTANCE AB Shiga toxin producing Escherichia coli (STEC) strains are important foodborne pathogens. Among these, E. coli O157:H7 is the most frequently isolated STEC serotype responsible for foodborne diseases. However, the non-O157 serotypes have been associated with serious outbreaks and sporadic diseases as well. It has been shown that various STEC serotypes are capable of forming biofilms on different food or food contact surfaces that, when detached, may lead to cross-contamination. Bacterial cells at biofilm stage also are more tolerant to sanitizers compared with their planktonic counterparts, which makes STEC biofilms a serious food safety concern. In the present study, we evaluated the potency of biofilm formation by a variety of STEC strains from serotypes O157:H7, O26:H11, and O111:H8; we also compared biofilm tolerance with two types of common sanitizers, a quaternary ammonium chloride based sanitizer and chlorine. Our results demonstrated that biofilm formation by various STEC serotypes on a polystyrene surface was highly strain-dependent, whereas the two non-O157 serotypes showed a higher potency of pellicle formation at air-liquid interfaces on a glass surface compared with serotype O157:H7. Significant reductions of viable biofilm cells were achieved with sanitizer treatments. STEC biofilm tolerance to sanitization was strain-dependent regardless of the serotypes. Curli expression appeared to play a critical role in STEC biofilm formation and tolerance to sanitizers. Our data indicated that multiple factors, including bacterial serotype and strain, surface materials, and other environmental conditions, could significantly affect STEC biofilm formation. The high potential for biofilm formation by various STEC serotypes, especially the strong potency of pellicle formation by the curli-positive non-O157 strains with high sanitization tolerance, might contribute to bacterial colonization on food contact surfaces, which may result in downstream product contamination. C1 [Wang, Rong; Bono, James L.; Kalchayanand, Norasak; Shackelford, Steven; Harhay, Dayna M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Wang, R (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM rong.wang@ars.usda.gov NR 50 TC 28 Z9 28 U1 6 U2 25 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2012 VL 75 IS 8 BP 1418 EP 1428 DI 10.4315/0362-028X.JFP-11-427 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 987JM UT WOS:000307413100007 PM 22856565 ER PT J AU Sulaiman, IM Anderson, M Oi, DH Simpson, S Kerdahi, K AF Sulaiman, Irshad M. Anderson, Mickey Oi, David H. Simpson, Steven Kerdahi, Khalil TI Multilocus Genetic Characterization of Two Ant Vectors (Group II "Dirty 22" Species) Known To Contaminate Food and Food Products and Spread Foodborne Pathogens SO JOURNAL OF FOOD PROTECTION LA English DT Article ID REGULATORY ACTION CRITERIA; PHYLOGENETIC ANALYSIS; FLIES DIPTERA; EVOLUTIONARY RELATIONSHIPS; ELONGATION FACTOR-1-ALPHA; MOLECULAR PHYLOGENY; INSECT PHYLOGENY; SP APICOMPLEXA; FILTH; SEQUENCES AB The U.S. Food and Drug Administration utilizes the presence of filth and extraneous materials as one of the criteria for implementing regulatory actions and assessing adulteration of food products of public health importance. Twenty-two prevalent pest species (also known as the "Dirty 22" species) have been considered by this agency as possible vehicles for the spread of foodborne diseases, and the presence of these species is considered an indicator of unsanitary conditions in food processing and storage facilities. In a previous study, we further categorized the Dirty 22 species into four groups: group I includes four cockroach species, group II includes two ant species, group III includes 12 fly species, and group IV includes four rodent species. Here, we describe the development of three nested PCR primer sets and multilocus genetic characterization by amplifying the small subunit rRNA, elongation factor 1-alpha, and wingless (WNT-1) genes of group II Dirty 22 ant species Monomorium pharaonis and Solenopsis molesta. These novel group II Dirty 22 species-specific nested PCR primer sets can be used when the specimens cannot be identified using conventional microscopic methods. These newly developed assays will provide correct identification of group II Dirty 22 ant species, and the information can be used in the control of foodborne pathogens. C1 [Sulaiman, Irshad M.; Anderson, Mickey; Simpson, Steven; Kerdahi, Khalil] US FDA, SE Reg Lab, Atlanta, GA 30309 USA. [Oi, David H.] USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Sulaiman, IM (reprint author), US FDA, SE Reg Lab, 60 8th St, Atlanta, GA 30309 USA. EM irshad.sulaiman@fda.hhs.gov FU Division of Field Sciences (DFS) of the FDA FX This study was supported in part by funding from the Division of Field Sciences (DFS) of the FDA. I. M. Sulaiman is the principal investigator of the DFS Challenge Initiative Program grant. The funders had no role in the study design, data collection, data analysis, decision to publish, or preparation of the manuscript. NR 49 TC 2 Z9 2 U1 0 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2012 VL 75 IS 8 BP 1447 EP 1452 DI 10.4315/0362-028X.JFP-12-098 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 987JM UT WOS:000307413100010 PM 22856568 ER PT J AU Alali, WQ Gaydashov, R Petrova, E Panin, A Tugarinov, O Kulikovskii, A Mamleeva, D Walls, I Doyle, MP AF Alali, Walid Q. Gaydashov, Roman Petrova, Elena Panin, Alexander Tugarinov, Oleg Kulikovskii, Alexander Mamleeva, Dzhemile Walls, Isabel Doyle, Michael P. TI Prevalence of Salmonella on Retail Chicken Meat in Russian Federation SO JOURNAL OF FOOD PROTECTION LA English DT Article ID POULTRY PRODUCTS; RAW POULTRY; CAMPYLOBACTER; CARCASSES; FOOD; PATHOGENS; SPP.; COLI AB The objective of this study was to estimate the prevalence of Salmonella on raw retail chicken meat in Russia. Broiler chicken carcasses (n = 698) were collected from three regions of Russia: central (i.e., Moscow area), northwest (i.e., St. Petersburg area), and southern (i.e., Krasnodar area). In each region, samples were collected to represent various cities and districts, as well as different types of retail stores and carcass storage temperatures (i.e., chilled and frozen). All chicken samples were analyzed for the presence of Salmonella using a whole-carcass rinse method. The overall Salmonella prevalence was 31.5%. There were significant differences (P < 0.05) in Salmonella prevalence by (i) region-29.3% (n = 464) in Moscow, 38.5% (n = 192) in St. Petersburg, and 23.8% (n = 42) in Krasnodar; (ii) retail store type-28.8% (n = 236) in hypermarkets, 31.9% (n = 260) in supermarkets (part of chain stores), 44.3% (n = 61) in independent supermarkets, 42.9% (n = 28) in independent minimarkets, and 26.6% (n = 113) in wet markets; and (iii) poultry company-34.3% (n = 545) on chickens produced by integrated companies compared with 22.9% (n = 118) on chickens produced by nonintegrated companies. Strategies such as good agriculture and management practices should be enhanced to reduce Salmonella prevalence on raw poultry in Russia and therefore increase the safety of chicken products. C1 [Alali, Walid Q.; Doyle, Michael P.] Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA. [Gaydashov, Roman; Petrova, Elena] Consumer Rights Protect Soc, Moscow 125284, Russia. [Panin, Alexander; Tugarinov, Oleg; Kulikovskii, Alexander] All Russian State Ctr Qual & Standardizat Vet Dru, Moscow 123022, Russia. [Mamleeva, Dzhemile] Leningrad Interreg Vet Lab, St Petersburg 196158, Russia. [Walls, Isabel] Natl Inst Food & Agr, USDA, Washington, DC 20250 USA. RP Alali, WQ (reprint author), Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA. EM walali@uga.edu OI Walls, Isabel/0000-0002-9643-8845 FU University of Georgia FX This research was supported by the project "Data Collection for Salmonella in Raw Poultry in Russia" of the University of Georgia. We thank the laboratory staff at Rosselhoznadzor in Moscow, St. Petersburg, and Krasnodar. We also thank Gerasim Poruchikov from the Consumer Rights Protection Society for assistance in collecting chicken samples. NR 21 TC 11 Z9 13 U1 0 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2012 VL 75 IS 8 BP 1469 EP 1473 DI 10.4315/0362-028X.JFP-12-080 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 987JM UT WOS:000307413100013 PM 22856571 ER PT J AU Glenn, LM Englen, MD Lindsey, RL Frank, JF Turpin, JE Berrang, ME Meinersmann, RJ Fedorka-Cray, PJ Frye, JG AF Glenn, Lashanda M. Englen, Mark D. Lindsey, Rebecca L. Frank, Joseph F. Turpin, Jennifer E. Berrang, Mark E. Meinersmann, Richard J. Fedorka-Cray, Paula J. Frye, Jonathan G. TI Analysis of Antimicrobial Resistance Genes Detected in Multiple-Drug-Resistant Escherichia coli Isolates from Broiler Chicken Carcasses SO MICROBIAL DRUG RESISTANCE LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; SALMONELLA-ENTERICA; ANTIBIOTIC-RESISTANCE; CLASS-1 INTEGRONS; FOOD ANIMALS; PLASMIDS; STRAINS; MICROARRAY; GENETICS; PCR AB Multi-drug-resistant (MDR) bacteria in food animals are a potential problem in both animal and human health. In this study, MDR commensal Escherichia coli isolates from poultry were examined. Thirty-two E. coli isolates from broiler carcass rinses were selected based on their resistance to aminoglycosides, beta-lactams, chloramphenicols, tetracyclines, and sulfonamide antimicrobials. Microarray analysis for the presence of antimicrobial resistance and plasmid genes identified aminoglycoside [aac(6), aac(3), aadA, aph, strA, and strB], beta-lactam (bla(AmpC), bla(TEM), bla(CMY), and bla(PSE-1)), chloramphenicol (cat, flo, and cmlA), sulfamethoxazole (sulI and sulII), tetracycline [tet(A), tet(C), tet(D), and tetR], and trimethoprim (dfrA) resistance genes. IncA/C plasmid core genes were detected in 27 isolates, while IncHI1 plasmid genes were detected in one isolate, indicating the likely presence of these plasmids. PCR assays for 18 plasmid replicon types often associated with MDR in Enterobacteriaceae also detected one or more replicon types in all 32 isolates. Class I integrons were investigated by PCR amplification of the integrase I gene, intI1, and the cassette region flanked by conserved sequences. Twenty-five isolates were positive for the intI1 gene, and class I integrons ranging in size from similar to 1,000 to 3,300 bp were identified in 19 of them. The presence of class I integrons, IncA/C plasmid genes, and MDR-associated plasmid replicons in the isolates indicates the importance of these genetic elements in the accumulation and potential spread of antimicrobial resistance genes in the microbial community associated with poultry. C1 [Frye, Jonathan G.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Res Ctr, Athens, GA 30605 USA. [Frank, Joseph F.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. RP Frye, JG (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jonathan.frye@ars.usda.gov RI Frye, Jonathan/I-6382-2013 OI Frye, Jonathan/0000-0002-8500-3395 NR 48 TC 13 Z9 14 U1 0 U2 20 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD AUG PY 2012 VL 18 IS 4 BP 453 EP 463 DI 10.1089/mdr.2011.0224 PG 11 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA 978LL UT WOS:000306742200016 PM 22385320 ER PT J AU Grunwald, NJ AF Gruenwald, Niklaus J. . TI Novel insights into the emergence of pathogens: the case of chestnut blight SO MOLECULAR ECOLOGY LA English DT Editorial Material DE exotic pathogen; invasion biology; keystone species; plant pathogen ID SUDDEN OAK DEATH; PHYTOPHTHORA-RAMORUM; CRYPHONECTRIA-PARASITICA; FUNGUS; CHYTRIDIOMYCOSIS; DIVERSITY; EVOLUTION; GENETICS; ECOLOGY; HOST AB Exotic, invasive pathogens have emerged repeatedly and continue to emerge to threaten the worlds forests. Ecosystem structure and function can be permanently changed when keystone tree species such as the American chestnut (Castanea dentata) are eliminated from a whole range by disease. The fungal ascomycete pathogen Cryphonectria parasitica is responsible for causing chestnut blight. Once the pathogen was introduced into the Eastern US, where chestnuts were predominant, chestnuts were all but eliminated. This pathogen is currently causing extensive damage in Europe. A study in this issue of Molecular Ecology sheds new light on the pattern and process of emergence of this devastating plant pathogen (Dutech 2012). The authors used microsatellite markers to investigate the evolutionary history of C.similar to parasitica populations introduced into North America and Europe. To infer sources of migrants and the migration events, the authors included putative source populations endemic to China and Japan, inferred potentially unsampled populations and conducted a multivariate population genetic and complex ABC analysis. Cryphonectria parasitica emerges as an example of an introduced pathogen with limited genotypic diversity and some admixture in the invaded ranges, yet repeated invasions into different areas of Europe and the United States. This work sheds new light on the emergence of C.similar to parasitica providing compelling evidence that this pathogen emerged by repeated migration and occasional admixture. C1 USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. EM grunwaln@science.oregonstate.edu RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 21 TC 1 Z9 2 U1 8 U2 71 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2012 VL 21 IS 16 BP 3896 EP 3897 DI 10.1111/j.1365-294X.2012.05597.x PG 2 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 980MN UT WOS:000306897500002 PM 22835047 ER PT J AU Suganuma, K Horiuchi, K Matsuda, H Cheng, HN Aoki, A Asakura, T AF Suganuma, Koto Horiuchi, Ken Matsuda, Hironori Cheng, H. N. Aoki, Akihiro Asakura, Tetsuo TI NMR analysis and chemical shift calculations of poly(lactic acid) dimer model compounds with different tacticities SO POLYMER JOURNAL LA English DT Article DE NMR; poly(lactic acid); quantum chemical calculation; stereoregularity ID GAUSSIAN-BASIS SETS; CORRELATED MOLECULAR CALCULATIONS; PROPENE-NORBORNENE COPOLYMERS; STEREOCOMPLEX FORMATION; PERTURBATION-THEORY; CRYSTAL-STRUCTURE; C-13 ASSIGNMENTS; WAVE-FUNCTIONS; ATOMS LI; POLYMERS AB In this work, poly(lactic acid) (PLA) dimer model compounds with different tacticities were synthesized and studied in detail by H-1 and C-13 nuclear magnetic resonance (NMR) in three solvents, deuterated chloroform (CDCl3)/CCl4 (20/80 v/v), CDCl3 and dimethyl sulfoxide -d(6). All of the peaks in the H-1 and C-13 NMR spectra were assigned with the help of two-dimensional NMR. Although the solvents were different, the tacticity splitting of the dimers showed no significant difference among the solvents. The chemical shifts were calculated and compared with experimental shifts to understand the origin of the tacticity splitting in PLA. Thus, a conformational energy calculation was first performed to determine the energetically stable states in isotactic and syndiotactic dimers by several quantum chemical calculation methods. The H-1 and C-13 chemical shifts were then calculated for each conformation of the model compounds by considering both the conformational energies of the predominant conformation and the chemical shift of each conformation. The observed tacticity splitting of the chemical shifts between isotactic and syndiotactic H-1 and C-13 NMR peaks of the dimers was reproduced particularly well, using the combination of Becke's three parameter hybrid method for conformational energy calculations and Hartree-Fock for chemical shift calculations. Polymer Journal (2012) 44, 838-844; doi:10.1038/pj.2012.106; published online 13 June 2012 C1 [Suganuma, Koto; Aoki, Akihiro; Asakura, Tetsuo] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan. [Suganuma, Koto; Horiuchi, Ken; Matsuda, Hironori] Teijin Ltd, Mat Anal Res Labs, Hino, Tokyo, Japan. [Cheng, H. N.] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. RP Asakura, T (reprint author), Tokyo Univ Agr & Technol, Dept Biotechnol, 2-24-16 Nakacho, Koganei, Tokyo 1848588, Japan. EM asakura@cc.tuat.ac.jp RI Asakura, Tetsuo/B-9970-2013 FU Ministry of Education, Science, Culture and Supports of Japan [23245045, 21550112] FX T.A. acknowledges support from a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Culture and Supports of Japan (23245045) and (21550112). We thank Mr Masato Komiyama at Teijin Pharma Ltd for his support on the synthesis of the model compounds and Dr Masao Hirasaka at Teijin Ltd for useful discussions. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture; the USDA is an equal opportunity provider and employer. NR 57 TC 3 Z9 3 U1 1 U2 21 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0032-3896 J9 POLYM J JI Polym. J. PD AUG PY 2012 VL 44 IS 8 SI SI BP 838 EP 844 DI 10.1038/pj.2012.106 PG 7 WC Polymer Science SC Polymer Science GA 987ZB UT WOS:000307456600016 ER PT J AU Pantin-Jackwood, MJ Smith, DM Wasilenko, JL Cagle, C Shepherd, E Sarmento, L Kapczynski, DR Afonso, CL AF Pantin-Jackwood, Mary J. Smith, Diane M. Wasilenko, Jamie L. Cagle, Caran Shepherd, Eric Sarmento, Luciana Kapczynski, Darrell R. Afonso, Claudio L. TI Effect of age on the pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses SO VIRUS RESEARCH LA English DT Article DE Ducks; Highly pathogenic avian influenza; H5N1; Pathogenesis; Innate immune response; Host gene expression ID DOMESTIC DUCKS; ANAS-PLATYRHYNCHOS; CYTOKINE RESPONSES; GENE-EXPRESSION; CAIRINA-MOSCHATA; EPITHELIAL-CELLS; INDUCED FEVER; NITRIC-OXIDE; A VIRUS; CHICKENS AB The pathogenicity of H5N1 highly pathogenic avian influenza (HPAI) viruses in domestic ducks varies between different viruses and is affected by the age of the ducks, with younger ducks presenting a more severe disease. In order to better understand the pathobiology of H5N1 HPAI in ducks including the role of host responses, 2 and 5-week-old Pekin ducks were infected with three different H5N1 HPAI viruses. Virus-induced pathology ranged from no clinical signs to severe disease and mortality, with the 2-week-old ducks being more severely affected by the more virulent viruses. However, these more virulent viruses induced higher body temperatures in the 5-week-old ducks than in the 2-week-old ducks indicating possible differences in innate immune responses. To analyze the ducks host responses to H5N1 HPAI virus infection, expression of innate immune-related genes was measured in the spleens and lungs of infected ducks at the peak of virus infection. IFN-alpha, RIG-I, and IL-6 RNA levels were increased in spleens regardless of the virus given and the age of the ducks, however differences were observed in the levels of up-regulation of IFN-alpha and RIG-I between the 2 and the 5-week-old ducks with the more virulent virus. Differences in IL-2 gene expression were also observed. In the lungs, the levels of expression of innate immune-related genes were lower than in the spleen, with mostly up-regulation of RIG-I and IL-6 and down-regulation of IFN-alpha and IL-2; no significant difference in expression was found between the 2 and the 5-week-old ducks. The differences observed in the innate immune responses to infection with H5N1 HPAI viruses could explain in part the differences in pathogenicity found between the 2 and 5-week-old ducks, however earlier time points after infection and additional innate immune-related genes should be examined. Published by Elsevier B.V. C1 [Pantin-Jackwood, Mary J.; Smith, Diane M.; Wasilenko, Jamie L.; Cagle, Caran; Shepherd, Eric; Sarmento, Luciana; Kapczynski, Darrell R.; Afonso, Claudio L.] ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM mary.pantin-jackwood@ars.usda.gov FU USDA/ARS CRIS project [6612-32000-048] FX The authors appreciate the assistance provided by Tracy Smith-Faulkner, Ronald Graham and Roger Brock in conducting these studies. The authors also thank the FSIS histopathology laboratory at Russell Research Center-USDA for technical assistance. This research was supported by USDA/ARS CRIS project #6612-32000-048. Mention of trade names or commercial products in this presentation is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 60 TC 16 Z9 17 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD AUG PY 2012 VL 167 IS 2 BP 196 EP 206 DI 10.1016/j.virusres.2012.04.015 PG 11 WC Virology SC Virology GA 980JJ UT WOS:000306889100009 PM 22595263 ER PT J AU Suo, SQGW Ren, YD Li, GX Zarlenga, D Bu, RE Su, DD Li, XL Li, PC Meng, FD Wang, C Ren, XF AF Suo, Siqingaowa Ren, Yudong Li, Guangxing Zarlenga, Dante Bu, Ri-e Su, Dingding Li, Xunliang Li, Pengchong Meng, Fandan Wang, Chao Ren, Xiaofeng TI Immune responses induced by DNA vaccines bearing Spike gene of PEDV combined with porcine IL-18 SO VIRUS RESEARCH LA English DT Article DE Porcine epidemic diarrhea virus; Spike protein; Porcine interleukin-18 gene; Adjuvant; DNA immunization ID TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; MESSENGER-RNA EXPRESSION; T-CELLS; OSTERTAGIA-OSTERTAGI; INTERFERON-GAMMA; IFN-GAMMA; CYTOKINE; VIRUS; INFECTION; INTERLEUKIN-18 AB Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea, a highly contagious enteric disease of swine. The Spike (S) protein is one of the main structural proteins of PEDV capable of inducing neutralizing antibodies in vivo. Herein, we generated three distinct DNA constructs in the eukaryotic expression plasmid pVAX1; one encoding the S protein [pVAX1-(PEDV-S)], the second encoding the N-terminal fragment (S1) [pVAX1-(PEDV-S1)] containing potent antigenic sites, and the third expressing the porcine interleukin-18 (pIL-18) [pVAX1-(IL-18)]. Immunofluorescence assays in BHK-21 cells demonstrated successful protein expression from all 3 constructs. Kunming mice were injected separately with each of these constructs or with a pVAX1-(PEDV-S1)/pVAX1-(IL-18) combination, an attenuated PEDV vaccine, or vector only control. Animals were examined for T lymphocyte proliferation, anti-PEDV antibodies, IFN-gamma and IL-4 protein levels, and cytotoxic T cell function in mouse peripheral blood and spleen. In all cases, results showed that pVAX1-(PEDV-S) and the combination of pVAX1-(PEDV-S1) with pVAX1-( IL-18) induced the strongest responses; however, pIL-18 had no adjuvant effects when given in combination with pVAX1-(PEDV-S1). (c) 2012 Elsevier B.V. All rights reserved. C1 [Suo, Siqingaowa; Ren, Yudong; Li, Guangxing; Li, Xunliang; Li, Pengchong; Meng, Fandan; Wang, Chao; Ren, Xiaofeng] NE Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China. [Suo, Siqingaowa; Bu, Ri-e; Ren, Xiaofeng] Inner Mongolia Univ Nationalities, Sch Life Sci, Tongliao 028043, Inner Mongolia, Peoples R China. [Zarlenga, Dante] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Su, Dingding] Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat G, Changsha 410128, Hunan, Peoples R China. RP Ren, XF (reprint author), NE Agr Univ, Coll Vet Med, 59 Mucai St, Harbin 150030, Peoples R China. EM renxf@neau.edu.cn FU Program for New Century Excellent Talents at the Heilongjiang Provincial University, China [1155-NCET-005]; National Natural Science Foundation of China [30972195, 31172295]; Program for New Century Excellent Talents in University of Ministry of Education of PR China [NCET-10-0144]; Key Project of Ministry of Education of PR China [212038]; Research Team Program on Scientific and Technological Innovation in Heilongjiang Provincial University [2011TD001] FX We acknowledge the Program for New Century Excellent Talents at the Heilongjiang Provincial University (1155-NCET-005), China. National Natural Science Foundation of China (30972195; 31172295), Program for New Century Excellent Talents in University of Ministry of Education of PR China (NCET-10-0144), the Key Project of Ministry of Education of PR China (212038) and Research Team Program on Scientific and Technological Innovation in Heilongjiang Provincial University (2011TD001). NR 23 TC 9 Z9 17 U1 1 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD AUG PY 2012 VL 167 IS 2 BP 259 EP 266 DI 10.1016/j.virusres.2012.05.007 PG 8 WC Virology SC Virology GA 980JJ UT WOS:000306889100015 PM 22643071 ER PT J AU Deng, QJ Song, MX Demers, A Weng, YJ Lu, WX Wang, D Kaushik, RS Yu, QZ Li, F AF Deng, Qiji Song, Minxun Demers, Andrew Weng, Yuejin Lu, Wuxun Wang, Dan Kaushik, Radhey S. Yu, Qingzhong Li, Feng TI Biochemical characterization of the small hydrophobic protein of avian metapneumovirus SO VIRUS RESEARCH LA English DT Article DE RNA viruses; Paramyxoviruses; Avian metapneumovirus; SH protein; Glycosylation; Homodimer ID RESPIRATORY-SYNCYTIAL-VIRUS; INTEGRAL MEMBRANE-PROTEIN; CELL-SURFACE EXPRESSION; SUBGROUP-C; SH PROTEIN; POLYLACTOSAMINOGLYCAN MODIFICATION; GLYCOPROTEIN-GPI; SEQUENCE; PNEUMOVIRUSES; PATHOGENESIS AB Avian metapneumovirus (AMPV) is a paramyxovirus that has three membrane proteins (G, F, and SH). Among them, the SH protein is a small type II integral membrane protein that is incorporated into virions and is only present in certain paramyxoviruses. In the present study, we show that the AMPV SH protein is modified by N-linked glycans and can be released into the extracellular environment. Furthermore, we demonstrate that glycosylated AMPV SH proteins form homodimers through cysteine-mediated disulfide bonds, which has not been reported previously for SH proteins of paramyxoviruses. (c) 2012 Elsevier B.V. All rights reserved. C1 [Deng, Qiji; Demers, Andrew; Weng, Yuejin; Lu, Wuxun; Wang, Dan; Kaushik, Radhey S.; Li, Feng] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [Deng, Qiji; Demers, Andrew; Weng, Yuejin; Lu, Wuxun; Wang, Dan; Kaushik, Radhey S.; Li, Feng] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Song, Minxun; Li, Feng] Shandong Acad Agr Sci, Inst Poultry Sci, Jinan 250023, Peoples R China. [Yu, Qingzhong] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Li, F (reprint author), S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. EM feng.li@sdstate.edu FU SD AES [3AH203]; NIH [5K02AI076125-02]; USDA, ARS CRIS project [6612-32000-056-00D] FX We thank Elizabeth Kolb for editing the manuscript and Xiuqin Xia for outstanding technical assistance. This work was supported by the SD AES (3AH203 to FL), NIH 5K02AI076125-02 award to FL, and USDA, ARS CRIS project 6612-32000-056-00D (to QY). NR 30 TC 0 Z9 0 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD AUG PY 2012 VL 167 IS 2 BP 297 EP 301 DI 10.1016/j.virusres.2012.05.013 PG 5 WC Virology SC Virology GA 980JJ UT WOS:000306889100019 PM 22659296 ER PT J AU van der Velde, M van der Eerden, BCJ Sun, YX Almering, JMM van der Lely, AJ Delhanty, PJD Smith, RG van Leeuwen, JPTM AF van der Velde, Martijn van der Eerden, Bram C. J. Sun, Yuxiang Almering, Julia M. M. van der Lely, Aart-Jan Delhanty, Patric J. D. Smith, Roy G. van Leeuwen, Johannes P. T. M. TI An Age-Dependent Interaction with Leptin Unmasks Ghrelin's Bone-Protective Effects SO ENDOCRINOLOGY LA English DT Article ID SYMPATHETIC-NERVOUS-SYSTEM; MINERAL DENSITY; SEXUAL-MATURITY; EXPRESSION; GROWTH; CELLS; DIFFERENTIATION; STIMULATION; METABOLISM; ACTIVATION AB The mutual interplay between energy homeostasis and bone metabolism is an important emerging concept. Ghrelin and leptin antagonize each other in regulating energy balance, but the role of this interaction in bone metabolism is unknown. Using ghrelin receptor and leptin-deficient mice, we show that ghrelin has dual effects on osteoclastogenesis, inhibiting osteoclast progenitors directly and stimulating osteoclastogenesis via a more potent systemic/central pathway. Using mice with combined ghrelin receptor and leptin deficiency, we find that this systemic osteoclastogenic activity is suppressed by leptin, thus balancing the two counterregulatory ghrelin pathways and leading to an unchanged bone structure. With aging, this osteoclastogenic ghrelin pathway is lost, unmasking the direct protective effect of ghrelin on bone structure. In conclusion, we identify a novel regulatory network linking orexigenic and anorectic metabolic factors with bone metabolism that is age dependent. (Endocrinology 153: 3593-3602, 2012) C1 [van der Velde, Martijn; van der Eerden, Bram C. J.; Almering, Julia M. M.; van der Lely, Aart-Jan; Delhanty, Patric J. D.; van Leeuwen, Johannes P. T. M.] Erasmus Univ, Med Ctr, Dept Internal Med, NL-3015 CE Rotterdam, Netherlands. [Sun, Yuxiang] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Smith, Roy G.] Scripps Res Inst Florida, Dept Metab & Aging, Jupiter, FL 33458 USA. RP van Leeuwen, JPTM (reprint author), Erasmus Univ, Med Ctr, Dept Internal Med, Gravendijkwal 230, NL-3015 CE Rotterdam, Netherlands. EM j.vanleeuwen@erasmusmc.nl FU Zorgonderzoek Nederland Medische Wetenschappen, The Netherlands [91203022] FX This work was supported by the Zorgonderzoek Nederland Medische Wetenschappen, The Netherlands, Program Grant 91203022. NR 34 TC 17 Z9 17 U1 0 U2 7 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 2012 VL 153 IS 8 BP 3593 EP 3602 DI 10.1210/en.2012-1277 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 977JD UT WOS:000306652400008 PM 22700774 ER PT J AU Hill, CB Chirumamilla, A Hartman, GL AF Hill, C. B. Chirumamilla, A. Hartman, G. L. TI Resistance and virulence in the soybean-Aphis glycines interaction SO EUPHYTICA LA English DT Review DE Soybean aphids; Soybean aphid biotypes; Soybean resistance ID RUSSIAN WHEAT APHID; CLASSICAL BIOLOGICAL-CONTROL; SINGLE DOMINANT GENE; HEMIPTERA-APHIDIDAE; NORTH-AMERICA; BIOTYPIC DIVERSITY; DEFENSE RESPONSES; FEEDING-BEHAVIOR; NATURAL ENEMIES; CONTROL AGENT AB Aphis glycines Matsumura, the soybean aphid, first arrived in North America in 2000 and has since become the most important insect pest of domestic soybean, causing significant yield loss and increasing production costs annually in many parts of the USA soybean belt. Research to identify sources of resistance to the pest began shortly after it was found and several sources were quickly identified in the USDA soybean germplasm collection. Characterization of resistance expression and mapping of resistance genes in resistant germplasm accessions resulted in the identification of six named soybean aphid resistance genes: Rag1, rag1c, Rag2, Rag3, rag4, and Rag5 (proposed). Simple sequence repeat markers flanking the resistance genes were identified, facilitating efforts to use marker-assisted selection to develop resistant commercial cultivars. Saturation or fine-mapping with single nucleotide polymorphism markers narrowed the genomic regions containing Rag1 and Rag2 genes. Two potential NBS-LRR candidate genes for Rag1 and one NBS-LRR gene for Rag2 were found within the regions. Years before the release of the first resistant soybean cultivar with Rag1 in 2009, a soybean aphid biotype, named biotype 2, was found that could overcome the resistance gene. Later in 2010, biotype 3 was characterized for its ability to colonize plants with Rag2 and other resistance genes. At present, three biotypes have been reported that can be distinguished by their virulence on Rag1 and Rag2 resistance genes. Frequency and geographic distribution of soybean aphid biotypes are unknown. Research is in progress to determine the inheritance of virulence and develop DNA markers tagging virulence genes to facilitate monitoring of biotypes. With these research findings and the availability of host lines with different resistance genes and biotypes, the soybean aphid-soybean pest-host system has become an important model system for advanced research into the interaction of an aphid with its plant host, and also the tritrophic interaction that includes aphid endosymbionts. C1 [Hill, C. B.; Chirumamilla, A.; Hartman, G. L.] Univ Illinois, Dept Crops Sci, Urbana, IL 61801 USA. [Hartman, G. L.] ARS, USDA, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crops Sci, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU Illinois Soybean Association; North Central Soybean Research Program; United Soybean Board FX We thank the following funding agencies for partial support of our research program: Illinois Soybean Association, North Central Soybean Research Program, and the United Soybean Board. We also thank Dr. David Voegtlin for his editorial comments and Theresa Herman for her overall review. We also appreciate the cooperative efforts of Drs. Brian Diers and Rosanna Giordano on soybean aphid breeding and genomics research, respectively. NR 98 TC 41 Z9 41 U1 4 U2 64 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD AUG PY 2012 VL 186 IS 3 BP 635 EP 646 DI 10.1007/s10681-012-0695-z PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 980YR UT WOS:000306930600005 ER PT J AU Porter, L AF Porter, Lyndon TI Selection of pea genotypes with partial resistance to Sclerotinia sclerotiorum across a wide range of temperatures and periods of high relative humidity SO EUPHYTICA LA English DT Article DE Pea; Pisum sativum; RH; Sclerotinia sclerotiorum; Screening techniques; Temperature; White mould ID DISEASES AB Partial resistance to Sclerotinia sclerotiorum based on stem lesion advancement was assessed for nine wild pea genotypes from five geographic origins and two cultivated genotypes, when peas were inoculated and incubated at all combinations of five temperatures (15.6, 18.3, 21.1, 23.9, 29.4A degrees C) and four period(s) of high relative humidity (PHRH; 12, 24, 48, 72 h). PHRH of 12 and 24 h should not be used when screening plants for resistance to S. sclerotiorum regardless of the incubation temperature, since stem lesions are rarely (2.7%) visible at 12 h and there were no significant differences (P a parts per thousand currency sign 0.05) in lesion lengths among and within genotypes at all temperatures assessed after 24 h. However, PHRH of 48 and 72 h are recommended for use to assess partial resistance since significant differences in stem lesion length among the genotypes were observed and characterized for these periods. Genotypes with cool (15.6 and 18.3A degrees C) versus warm (23.9 and 29.4A degrees C) temperature partial resistance to S. sclerotiorum were identified, and genotypes PI 240515 and PI 169603 appear to have the best cool and warm temperature partial resistance, respectively, among the genotypes assessed. A temperature of 21.1A degrees C was the optimal temperature favouring lesion advancement for the majority of the genotypes evaluated. PI 169603 demonstrated the best partial resistance to S. sclerotiorum across the widest temperature and PHRH ranges and is recommended to plant breeders as the best single genotype to develop future cultivars with improved partial resistance to S. sclerotiorum based on stem lesion advancement. C1 ARS, Vegetable & Forage Crops Res Unit, USDA, Prosser, WA USA. RP Porter, L (reprint author), ARS, Vegetable & Forage Crops Res Unit, USDA, 24106 N Bunn Rd, Prosser, WA USA. EM lyndon.porter@ars.usda.gov FU National Sclerotinia Initiative of the USDA-ARS FX The author would like to thank the National Sclerotinia Initiative of the USDA-ARS for funding this research and Dr. Clare Coyne and personnel at the USDA WRPIS for providing the seed for this research project. The author would also like to thank Virginia A. Coffman and Andrea De Vries for their technical help in completing this research and Drs. Zahi Akannan Atallah and Nadav Nitzan for their critical reviews. Finally, the author would like to thank Dr. Marc Evans, Professor of Statistics, at Washington State University for his assistance with the statistical analyses. NR 11 TC 0 Z9 0 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD AUG PY 2012 VL 186 IS 3 BP 671 EP 678 DI 10.1007/s10681-011-0531-x PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 980YR UT WOS:000306930600008 ER PT J AU Perez-Sackett, PT Palmer, RG AF Perez-Sackett, P. T. Palmer, R. G. TI Effect of day and night temperature on the expression of male sterility of nuclear male-sterile (ms8ms8) soybean SO EUPHYTICA LA English DT Article DE Soybean; Sterility; Temperature-effects ID HETEROSIS; PERFORMANCE; STRAIN AB In soybean [Glycine max (L.) Merr.], an environmentally stable male-sterility system is one of the requirements to produce large quantities of hybrid seed. Observations of the nuclear male-sterile, female-fertile mutant ms8ms8 in different environments suggested that environmental conditions affected expressivity of the ms8 allele with respect to male sterility. The objective of this study was to determine the effect of day and night temperatures on the expression of male sterility of the ms8 ms8 genotype. Eighteen male-sterile, female-fertile ms8ms8 plants and ten fertile siblings Ms8_ were grown in growth chambers with the following day/night temperatures regimes: 20/20, 24/18, 25/19, 25/20, 27/21, 28/25, 29/17, 29/24, 30/25, 30/30, 32/25, and 35/25A degrees C. Stability of the ms8 allele was affected by the different temperature regimes. Increased sterility of the ms8ms8 genotype was observed when night temperature was 25A degrees C and day temperature varied between 30 and 35A degrees C. We found optimal temperature regimes for sterility expression of the ms8 mutant. These regimes could be used for hybrid seed production when using insect-mediated cross pollination. C1 [Palmer, R. G.] Iowa State Univ, USDA, ARS, CICGR, Ames, IA 50011 USA. [Perez-Sackett, P. T.; Palmer, R. G.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Palmer, RG (reprint author), Iowa State Univ, USDA, ARS, CICGR, Ames, IA 50011 USA. EM rpalmer@iastate.edu NR 27 TC 2 Z9 3 U1 0 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD AUG PY 2012 VL 186 IS 3 BP 847 EP 853 DI 10.1007/s10681-012-0629-9 PG 7 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 980YR UT WOS:000306930600024 ER PT J AU Mietus-Snyder, ML Shigenaga, MK Suh, JH Shenvi, SV Lal, A McHugh, T Olson, D Lilienstein, J Krauss, RM Gildengoren, G McCann, JC Ames, BN AF Mietus-Snyder, Michele L. Shigenaga, Mark K. Suh, Jung H. Shenvi, Swapna V. Lal, Ashutosh McHugh, Tara Olson, Don Lilienstein, Joshua Krauss, Ronald M. Gildengoren, Ginny McCann, Joyce C. Ames, Bruce N. TI A nutrient-dense, high-fiber, fruit-based supplement bar increases HDL cholesterol, particularly large HDL, lowers homocysteine, and raises glutathione in a 2-wk trial SO FASEB JOURNAL LA English DT Article DE antioxidant status; cardiovascular disease risk; dyslipidemia; lipid particles; obesity ID CARDIOVASCULAR RISK-FACTORS; POLYUNSATURATED FATTY-ACIDS; CORONARY-HEART-DISEASE; RANDOMIZED CONTROLLED-TRIALS; METABOLIC SYNDROME; LIPOPROTEIN CHOLESTEROL; INSULIN-RESISTANCE; FOLIC-ACID; OXIDATIVE STRESS; GLYCEMIC INDEX AB Dietary intake modulates disease risk, but little is known how components within food mixtures affect pathophysiology. A low-calorie, high-fiber, fruit-based nutrient-dense bar of defined composition (e. g., vitamins and minerals, fruit polyphenolics, beta-glucan, docosahexaenoic acid) appropriate for deconstruction and mechanistic studies is described and evaluated in a pilot trial. The bar was developed in collaboration with the U. S. Department of Agriculture. Changes in cardiovascular disease and diabetes risk biomarkers were measured after 2 wk twice-daily consumption of the bar, and compared against baseline controls in 25 healthy adults. Plasma HDL-cholesterol (HDL-c) increased 6.2% (P = 0.001), due primarily to a 28% increase in large HDL (HDL-L; P<0.0001). Total plasma homocysteine (Hcy) decreased 19% (P = 0.017), and glutathione (GSH) increased 20% (P = 0.011). The changes in HDL and Hcy are in the direction associated with decreased risk of cardiovascular disease and cognitive decline; increased GSH reflects improved antioxidant defense. Changes in biomarkers linked to insulin resistance and inflammation were not observed. A defined food-based supplement can, within 2 wk, positively impact metabolic biomarkers linked to disease risk. These results lay the groundwork for mechanistic/deconstruction experiments to identify critical bar components and putative synergistic combinations responsible for observed effects.-Mietus-Snyder, M. L., Shigenaga, M. K., Suh, J. H., Shenvi, S. V., Lal, A., McHugh, T., Olson, D., Lilienstein, J., Krauss, R. M., Gildengoren, G., McCann, J. C., Ames, B. N. A nutrient-dense, high-fiber, fruit-based supplement bar increases HDL cholesterol, particularly large HDL, lowers homo-cysteine, and raises glutathione in a 2-wk trial. FASEB J. 26, 3515-3527 (2012). www.fasebj.org C1 [Mietus-Snyder, Michele L.; Shigenaga, Mark K.; Suh, Jung H.; Shenvi, Swapna V.; Lal, Ashutosh; Krauss, Ronald M.; Gildengoren, Ginny; McCann, Joyce C.; Ames, Bruce N.] Childrens Hosp Oakland Res Inst, Nutr & Metab Ctr, Oakland, CA 94609 USA. [McHugh, Tara; Olson, Don] ARS, Proc Foods Res Unit, USDA, Western Reg Res Ctr, Albany, CA USA. RP Shigenaga, MK (reprint author), Childrens Hosp Oakland Res Inst, Nutr & Metab Ctr, 5700 Martin Luther King Jr Way, Oakland, CA 94609 USA. EM mshigenaga@chori.org; bames@chori.org FU U.S. National Institutes of Health [UL1RR024131]; Bruce and Giovanna Ames Foundation; Department of Atherosclerosis Research at Children's Hospital Oakland Research Institute; S. D. Bechtel, Jr. Foundation FX Many thanks to Alicia Zhou, Casey Geaney, Katie Woojinski, and Devan Block for technical assistance, the extraordinary staff at the Pediatric Clinical Research Center (PCRC) at Children's Hospital Oakland, especially Annie Higa and Vivian Ng. M. K. S. thanks Wally Yokoyama for helpful discussions on soluble fibers. The authors also thank Pharmachem Laboratories (Kearny, NJ, USA) for donating a nutrient blend for the study and Teresa Klask for invaluable administrative assistance. This publication was made possible by U.S. National Institutes of Health Clinical and Translational Science Award grant UL1RR024131. Additional support was from the Bruce and Giovanna Ames Foundation (J.C.M., M. M. S., M. K. S., J.H.S., S. V. S., A. L.), the Department of Atherosclerosis Research at Children's Hospital Oakland Research Institute (R. M. K.), and the S. D. Bechtel, Jr. Foundation (J.H.S.). NR 175 TC 6 Z9 6 U1 4 U2 22 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD AUG PY 2012 VL 26 IS 8 BP 3515 EP 3527 DI 10.1096/fj.11-201558 PG 13 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 984BI UT WOS:000307162800038 PM 22549511 ER PT J AU Dunn, RO AF Dunn, Robert O. TI Effects of Monoacylglycerols on the Cold Flow Properties of Biodiesel SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Cloud point; Cold filter plugging point; Differential scanning calorimetry; Freezing point; Kinematic viscosity; Refractive index; Soybean oil-FAME; Specific gravity; Temperature-composition phase diagram; Wax appearance point ID VEGETABLE-OILS; SOYBEAN OIL; BLENDS; FUELS AB Biodiesel is a renewable alternative fuel made from plant oils and animal fats that may be burned in a compression-ignition (diesel) engine. It is composed of mono-alkyl fatty acid esters made from plant oils or animal fats mainly by transesterification with methanol or ethanol. This process leaves behind small concentrations of minor constituents including monoacylglycerols (MAG). Saturated MAG have low solubility in biodiesel and may form solid residues during storage in cold weather. Soybean oil-fatty acid methyl esters (SME) were mixed with up to 1.0 mass% MAG to evaluate the effects on cloud point (CP), freezing point (FP), cold filter plugging point (CFPP), and wax appearance point (WAP). Differential scanning calorimetry (DSC) results showed that MAG with only 27.6 mass% total long chain (C16-C18) saturated fatty acid content had melting transitions between 54 and 59.0 A degrees C. Furthermore, DSC analysis indicated that pure monoolein may be problematic with respect to melting transitions between 25.4 and 33.4 A degrees C. Solubility data for SME-MAG mixtures indicated a broad transition temperature range from solid at low temperature to liquid at temperatures exceeding 60 A degrees C. Increasing the added MAG content from 0.10 to 1.0 mass% increased both CP and FP. Cold filter plugging point demonstrated higher sensitivity than CP or FP at added MAG content below 0.10 mass%, though it was not affected by increasing MAG concentration above 0.50 mass%. Wax appearance point showed no effects until added MAG content exceeded 0.25 mass%. Kinematic viscosity measured at 5 A degrees C similarly showed no effects until added MAG concentration exceeded 0.20 mass%. Specific gravity at 15.6 A degrees C and refractive index at 25 A degrees C were not greatly affected by added MAG except at concentrations greater than 0.10 mass%. C1 ARS, Biooils Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Dunn, RO (reprint author), ARS, Biooils Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. EM Robert.Dunn@ars.usda.gov NR 27 TC 10 Z9 10 U1 1 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2012 VL 89 IS 8 BP 1509 EP 1520 DI 10.1007/s11746-012-2045-7 PG 12 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 979BE UT WOS:000306789100016 ER PT J AU Compton, DL Eller, FJ Laszlo, JA Evans, KO AF Compton, David L. Eller, Fred J. Laszlo, Joseph A. Evans, Kervin O. TI Purification of 2-Monoacylglycerols Using Liquid CO2 Extraction SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Acyl migration; Liquid CO2; 2-Monoacylglycerol; NMR; Structured lipids ID SUPERCRITICAL CARBON-DIOXIDE; ACYL MIGRATION KINETICS; STRUCTURED LIPIDS; FLUID EXTRACTION; SOYBEAN OIL; LIPASE; GLYCERIDES; ACID; FRACTIONATION; ETHANOLYSIS AB The fatty acid moiety of 2-monoacyl-sn-glycerol (2-MAG) undergoes spontaneous acyl migration to the sn-1(3) position, resulting in a thermodynamic equilibrium of approximately 1:9 of 2-MAG to 1(3)-monoacyl-sn-glycerol (1-MAG). Spontaneous acyl migration is an impediment to synthesizing and isolating specific 2-MAG for use as intermediates in the synthesis of structured triacylglycerols. 2-Monooleoyl-sn-glycerol was synthesized by the enzymatic ethanolysis of triolein and isolated by liquid CO2 extraction. The resultant MAG, diacylglycerol, and fatty acid ethyl esters were quantified by H-1 NMR and supercritical fluid chromatography. The low polarity of the CO2 and mild extraction temperature (25 A degrees C) resulted in very low spontaneous acyl migration rates, allowing the MAG to be isolated in 80% yield and in a very high 2-MAG:1-MAG ratios of a parts per thousand yen93 mol%. C1 [Compton, David L.; Laszlo, Joseph A.; Evans, Kervin O.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Eller, Fred J.] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Compton, DL (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM david.compton@ars.usda.gov NR 28 TC 4 Z9 4 U1 1 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2012 VL 89 IS 8 BP 1529 EP 1536 DI 10.1007/s11746-012-2035-9 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 979BE UT WOS:000306789100018 ER PT J AU Cheng, HN Dowd, MK Easson, MW Condon, BD AF Cheng, H. N. Dowd, Michael K. Easson, Michael W. Condon, Brian D. TI Hydrogenation of Cottonseed Oil with Nickel, Palladium and Platinum Catalysts SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Cottonseed oil; Hydrogenation; Nickel catalyst; Palladium catalyst; Platinum catalyst; trans Fatty acids ID LOW-TRANS; FATTY-ACIDS; SOYBEAN OIL; KINETICS; PRESSURE AB Eleven catalysts based on Ni, Pd, and Pt from two manufacturers were used for hydrogenating cottonseed oil, with the goal of minimizing trans fatty acid (TFA) content. Despite different temperatures, catalyst levels, and reaction times being used, the data from each catalyst type fell on the same curve when the TFA level was plotted against the iodine value (IV). Under low-pressure, high-mixing hydrogenation conditions, the Ni, Pd, and Pt catalysts all produced < 5% TFA and < 5% stearic acid at 95 IV; however, at 70 IV, only Pt catalysts produced 9% TFA and about 17% stearic acid. Kinetic modeling provided a better understanding of the relative reaction rates involved in hydrogenation. Pt catalysts converted linoleic and oleic acids more slowly to TFA and quickly hydrogenate TFA, thereby leading to low TFA levels. In comparison to other hydrogenated oils reported in the literature, equivalent or lower TFA and stearic acid levels were observed with hydrogenated cottonseed oil under the experimental conditions used. C1 [Cheng, H. N.; Dowd, Michael K.; Easson, Michael W.; Condon, Brian D.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Cheng, HN (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM hn.cheng@ars.usda.gov NR 32 TC 9 Z9 9 U1 5 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2012 VL 89 IS 8 BP 1557 EP 1566 DI 10.1007/s11746-012-2036-8 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 979BE UT WOS:000306789100021 ER PT J AU Nelson, MI Vincent, AL Kitikoon, P Holmes, EC Gramer, MR AF Nelson, Martha I. Vincent, Amy L. Kitikoon, Pravina Holmes, Edward C. Gramer, Marie R. TI Evolution of Novel Reassortant A/H3N2 Influenza Viruses in North American Swine and Humans, 2009-2011 SO JOURNAL OF VIROLOGY LA English DT Article ID PANDEMIC H1N1 2009; A H3N2 VIRUS; UNITED-STATES; 2 CHILDREN; PIGS; ORIGIN; TRANSMISSION; PERSPECTIVE; ARGENTINA; INFECTION AB Novel H3N2 influenza viruses (H3N2v) containing seven genome segments from swine lineage triple-reassortant H3N2 viruses and a 2009 pandemic H1N1 (H1N1 pdm09) matrix protein segment (pM) were isolated from 12 humans in the United States between August and December 2011. To understand the evolution of these novel H3N2 viruses in swine and humans, we undertook a phylogenetic analysis of 674 M sequences and 388 HA and NA sequences from influenza viruses isolated from North American swine during 2009-2011, as well as HA, NA, and M sequences from eight H3N2v viruses isolated from humans. We identified 34 swine influenza viruses (termed rH3N2p) with the same combination of H3, N2, and pM segments as the H3N2v viruses isolated from humans. Notably, these rH3N2p viruses were generated in swine via reassortment events between H3N2 viruses and the pM segment approximately 4 to 10 times since 2009. The pM segment has also reassorted with multiple distinct lineages of HI virus, especially H1 delta viruses. Importantly, the N2 segment of all H3N2v viruses isolated from humans is derived from a genetically distinct N2 lineage that has circulated in swine since being acquired by reassortment with seasonal human H3N2 viruses in 2001-2002, rather than from the N2 that is associated with the 1998 H3N2 swine lineage. The identification of this N2 variant may have implications for influenza vaccine design and the potential pandemic threat of H3N2v to human age groups with differing levels of prior exposure and immunity. C1 [Nelson, Martha I.; Holmes, Edward C.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Vincent, Amy L.; Kitikoon, Pravina] USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Holmes, Edward C.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Gramer, Marie R.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. RP Nelson, MI (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM nelsonma@mail.nih.gov OI Holmes, Edward/0000-0001-9596-3552 FU Office of Global Affairs at the Department of Health and Human Services (DHHS) FX This research was conducted within the context of the Multinational Influenza Seasonal Mortality Study (MISMS), an on-going international collaborative effort to understand influenza epidemiology and evolution, led by the Fogarty International Center, NIH, with funding from the Office of Global Affairs at the Department of Health and Human Services (DHHS). NR 39 TC 67 Z9 67 U1 2 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 2012 VL 86 IS 16 BP 8872 EP 8878 DI 10.1128/JVI.00259-12 PG 7 WC Virology SC Virology GA 984NS UT WOS:000307198300051 PM 22696653 ER PT J AU Taylor, A AF Taylor, Allen TI Introduction to the issue regarding research regarding age related macular degeneration SO MOLECULAR ASPECTS OF MEDICINE LA English DT Editorial Material DE Introduction C1 Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. RP Taylor, A (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, 711 Washington St, Boston, MA 02111 USA. EM allen.taylor@tufts.edu FU NEI NIH HHS [R01 EY 13250, R01 EY021212, R01 EY013250]; PHS HHS [R01 21212] NR 0 TC 3 Z9 3 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD AUG PY 2012 VL 33 IS 4 SI SI BP 291 EP 294 DI 10.1016/j.mam.2012.04.002 PG 4 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 983WR UT WOS:000307149900001 PM 22542402 ER PT J AU Weikel, KA Chiu, CJ Taylor, A AF Weikel, Karen A. Chiu, Chung-Jung Taylor, Allen TI Nutritional modulation of age-related macular degeneration SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE AMD; Antioxidants; Carotenoids; Nutrition; Glycemic index; Aging ID BLUE-MOUNTAINS-EYE; RETINAL-PIGMENT EPITHELIUM; ENDOTHELIAL GROWTH-FACTOR; DIETARY GLYCEMIC INDEX; 3RD NATIONAL-HEALTH; SUBFOVEAL CHOROIDAL NEOVASCULARIZATION; POLYUNSATURATED FATTY-ACIDS; HIGH-DOSE SUPPLEMENTATION; FACTOR-H POLYMORPHISM; BEAVER DAM EYE AB Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly worldwide. It affects 30-50 million individuals and clinical hallmarks of AMD are observed in at least one third of persons over the age of 75 in industrialized countries (Gehrs et al., 2006). Costs associated with AMD are in excess of $340 billion US (American-Health-Assistance-Foundation, 2012). The majority of AMD patients in the United States are not eligible for clinical treatments (Biarnes et al., 2011; Klein et al., 2011). Preventive interventions through dietary modulation are attractive strategies because many studies suggest a benefit of micro- and macronutrients with respect to AMD, as well as other age-related debilities, and with few, if any, adverse effects (Chiu, 2011). Preservation of vision would enhance quality of life for millions of elderly people, and alleviate the personal and public health financial burden of AMD (Frick et al., 2007; Wood et al., 2011). Observational studies indicate that maintaining adequate levels of omega-3 fatty acids (i.e. with 2 servings/week of fish) or a low glycemic index diet may be particularly beneficial for early AMD and that higher levels of carotenoids may be protective, most probably, against neovascular AMD. Intervention trials are needed to better understand the full effect of these nutrients and/or combinations of nutrients on retinal health. Analyses that describe effects of a nutrient on onset and/or progress of AMD are valuable because they indicate the value of a nutrient to arrest AMD at the early stages. This comprehensive summary provides essential information about the value of nutrients with regard to diminishing risk for onset or progress of AMD and can serve as a guide until data from ongoing intervention trials are available. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Weikel, Karen A.; Chiu, Chung-Jung; Taylor, Allen] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. RP Taylor, A (reprint author), Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, 711 Washington St, Boston, MA 02111 USA. EM allen.taylor@tufts.edu FU USDA [1950-510000-060-01A]; Johnson and Johnson Focused Giving; NIH [RO1 13250, RO1 21212] FX This research was funded by USDA 1950-510000-060-01A, Johnson and Johnson Focused Giving, NIH Grant RO1 13250, NIH Grant RO1 21212. These sponsors were not involved in data collection or interpretation or in writing this manuscript. NR 184 TC 26 Z9 27 U1 4 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD AUG PY 2012 VL 33 IS 4 SI SI BP 318 EP 375 DI 10.1016/j.mam.2012.03.005 PG 58 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 983WR UT WOS:000307149900003 PM 22503690 ER PT J AU Shang, F Taylor, A AF Shang, Fu Taylor, Allen TI Roles for the ubiquitin-proteasome pathway in protein quality control and signaling in the retina: Implications in the pathogenesis of age-related macular degeneration SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE Protein quality control; Signal transduction; Aging; Oxidative stress; Inflammation; Retina degeneration; Macula; Proteolysis ID NF-KAPPA-B; PIGMENT EPITHELIAL-CELLS; ANTIOXIDANT RESPONSE ELEMENT; TRANSCRIPTION FACTOR NRF2; TRANSMEMBRANE CONDUCTANCE REGULATOR; DROSOPHILA EYE DEVELOPMENT; FAT-FACETS GENE; 26 S-PROTEASOME; AMYOTROPHIC-LATERAL-SCLEROSIS; GLYCATION END-PRODUCTS AB The accumulation of damaged or postsynthetically modified proteins and dysregulation of inflammatory responses and angiogenesis in the retina/RPE are thought be etiologically related to formation of drusen and choroidal neovascularization (CNV), hallmarks of age-related macular degeneration (AMD). The ubiquitin-proteasome pathway (UPP) plays crucial roles in protein quality control, cell cycle control and signal transduction. Selective degradation of aberrant proteins by the UPP is essential for timely removal of potentially cytotoxic damaged or otherwise abnormal proteins. Proper function of the UPP is thought to be required for cellular function. In contrast, age - or stress induced - impairment the UPP or insufficient UPP capacity may contribute to the accumulation of abnormal proteins, cytotoxicity in the retina, and AMD. Crucial roles for the UPP in eye development, regulation of signal transduction, and antioxidant responses are also established. Insufficient UPP capacity in retina and RPE can result in dysregulation of signal transduction, abnormal inflammatory responses and CNV. There are also interactions between the UPP and lysosomal proteolytic pathways (LPPs). Means that modulate the proteolytic capacity are making their way into new generation of pharmacotherapies for delaying age-related diseases and may augment the benefits of adequate nutrition, with regard to diminishing the burden of AMD. (C) 2012 Published by Elsevier Ltd. C1 [Shang, Fu; Taylor, Allen] USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. RP Shang, F (reprint author), USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, 711 Washington St, Boston, MA 02111 USA. EM fu.shang@tufts.edu FU NIH [EY 011717, EY 013250, EY 021212]; USDA AFRI [2009-35200-05014]; USDA [1950-510000-060-01A]; AHAF [M2010038]; Dennis L. Gierhart Charitable Gift Fund FX This work is partially supported by NIH Grants EY 011717, EY 013250, EY 021212, USDA AFRI Award 2009-35200-05014, USDA contract 1950-510000-060-01A, AHAF Grant M2010038, Dennis L. Gierhart Charitable Gift Fund, and a gift from Alcon laboratories. NR 339 TC 14 Z9 15 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 EI 1872-9452 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD AUG PY 2012 VL 33 IS 4 SI SI BP 446 EP 466 DI 10.1016/j.mam.2012.04.001 PG 21 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 983WR UT WOS:000307149900008 PM 22521794 ER PT J AU Bono, JL Smith, TPL Keen, JE Harhay, GP McDaneld, TG Mandrell, RE Jung, WK Besser, TE Gerner-Smidt, P Bielaszewska, M Karch, H Clawson, ML AF Bono, James L. Smith, Timothy P. L. Keen, James E. Harhay, Gregory P. McDaneld, Tara G. Mandrell, Robert E. Jung, Woo Kyung Besser, Thomas E. Gerner-Smidt, Peter Bielaszewska, Martina Karch, Helge Clawson, Michael L. TI Phylogeny of Shiga Toxin-Producing Escherichia coli O157 Isolated from Cattle and Clinically Ill Humans SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE STEC O157; phylogeny; evolution; disease; cattle; human ID FIELD GEL-ELECTROPHORESIS; HEMOLYTIC-UREMIC SYNDROME; SINGLE-NUCLEOTIDE POLYMORPHISMS; MULTIPLEX PCR ASSAYS; MONOCLONAL-ANTIBODIES; MOLECULAR-DETECTION; VIRULENCE FACTORS; GENOME SEQUENCE; STRAINS; BOVINE AB Cattle are a major reservoir for Shiga toxin-producing Escherichia coli O157 (STEC O157) and harbor multiple genetic subtypes that do not all associate with human disease. STEC O157 evolved from an E. coli O55:H7 progenitor; however, a lack of genome sequence has hindered investigations on the divergence of human- and/or cattle-associated subtypes. Our goals were to 1) identify nucleotide polymorphisms for STEC O157 genetic subtype detection, 2) determine the phylogeny of STEC O157 genetic subtypes using polymorphism-derived genotypes and a phage insertion typing system, and 3) compare polymorphism-derived genotypes identified in this study with pulsed field gel electrophoresis (PFGE), the current gold standard for evaluating STEC O157 diversity. Using 762 nucleotide polymorphisms that were originally identified through whole-genome sequencing of 189 STEC O157 human- and cattle-isolated strains, we genotyped a collection of 426 STEC O157 strains. Concatenated polymorphism alleles defined 175 genotypes that were tagged by a minimal set of 138 polymorphisms. Eight major lineages of STEC O157 were identified, of which cattle are a reservoir for seven. Two lineages regularly harbored by cattle accounted for the majority of human disease in this study, whereas another was rarely represented in humans and may have evolved toward reduced human virulence. Notably, cattle are not a known reservoir for E. coli O55:H7 or STEC O157:H- (the first lineage to diverge within the STEC O157 serogroup), which both cause human disease. This result calls into question how cattle may have originally acquired STEC O157. The polymorphism-derived genotypes identified in this study did not surpass PFGE diversity assessed by BlnI and XbaI digestions in a subset of 93 strains. However, our results show that they are highly effective in assessing the evolutionary relatedness of epidemiologically unrelated STEC O157 genetic subtypes, including those associated with the cattle reservoir and human disease. C1 [Bono, James L.; Smith, Timothy P. L.; Harhay, Gregory P.; McDaneld, Tara G.; Clawson, Michael L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Keen, James E.] Univ Nebraska, Great Plains Vet Educ Ctr, Lincoln, NE 68583 USA. [Mandrell, Robert E.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. [Jung, Woo Kyung; Besser, Thomas E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Gerner-Smidt, Peter] Ctr Dis Control & Prevent, Enter Dis Lab, Response Branch, Atlanta, GA USA. [Bielaszewska, Martina; Karch, Helge] Univ Munster, Inst Hyg, Munster, Germany. [Bielaszewska, Martina; Karch, Helge] Univ Munster, Natl Consulting Lab Haemolyt Uraem Syndrome, Munster, Germany. RP Clawson, ML (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. EM mike.clawson@ars.usda.gov OI Clawson, Michael/0000-0002-3355-5390 FU Agricultural Research Service FX We thank Gennie Schuller-Chavez, Sandy Fryda-Bradley, Renee Godtel, Linda Flathman, Bob Lee, Kevin Tennill, and Jacky Carnahan for outstanding technical support for this project; Jim Wray, Phil Anderson, and Randy Bradley for computer support; and Joan Rosch for secretarial support. The use of product and company names is necessary to accurately report the methods and results; however, the United States Department of Agriculture (USDA) neither guarantees nor warrants the standard of the products, and the use of names by the USDA implies no approval of the product to the exclusion of others that may also be suitable. The USDA is an equal opportunity provider and employer. This work was supported by the Agricultural Research Service to J.L.B., T.P.L.S., G.P.H., T.G.M., R.E.M., and M.L.C. This research was conducted at the USDA, Agricultural Research Service (ARS), US Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, Nebraska, United States. NR 69 TC 23 Z9 25 U1 0 U2 12 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0737-4038 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD AUG PY 2012 VL 29 IS 8 BP 2047 EP 2062 DI 10.1093/molbev/mss072 PG 16 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 984EA UT WOS:000307171300015 PM 22355013 ER PT J AU Qi, LL Gulya, TJ Hulke, BS Vick, BA AF Qi, L. L. Gulya, T. J. Hulke, B. S. Vick, B. A. TI Chromosome location, DNA markers and rust resistance of the sunflower gene R-5 SO MOLECULAR BREEDING LA English DT Article DE Helianthus annuus L.; Sunflower; Puccinia helianthi; Rust; Simple sequence repeat; Gene R-5 ID LEAF RUST; PUCCINIA-HELIANTHI; DOWNY MILDEW; CULTIVATED SUNFLOWER; RICH REGIONS; LINKAGE MAP; WHEAT; IDENTIFICATION; REGISTRATION; GENOME AB Sunflower rust, caused by the fungus Puccinia helianthi Schwein., was not a serious problem for many decades because of successful deployment of effective resistance genes in commercial sunflower (Helianthus annuus L.) hybrids in North America. In the 1980s and early 1990s, however, a shift in virulence of the rust race population in North America rendered most of the commercial hybrids susceptible to new virulent races. A germplasm line, HA-R2, carrying the rust resistance gene R (5) was released as a multi-race rust-resistant line in 1985 but has not been widely used in commercial hybrid production. R (5) remains effective against the prevalent rust races of sunflower in North America. This gene was previously reported to be associated with two simple sequence repeat (SSR) markers, ORS316 and ORS630, which were mapped to linkage group (LG) 13 of sunflower. However, out of the 63 markers of LG13 screened in the present study, only 18, including ORS316 and ORS630, were polymorphic. These markers, which covered all of LG 13, were assayed in 94 individual F2 progenies derived from the cross of HA 89 with HA-R2. All failed to detect any locus in LG13 associated with the gene R (5) . Subsequently, a bulked segregant analysis was employed with an additional 510 SSR markers selected from the remaining 16 LGs of the sunflower genome. This analysis demonstrated that the LG2 markers showed association with rust resistance. Genotyping of the 94 F2 individuals with 23 polymorphic SSR markers from LG2 confirmed the R (5) location on LG2, flanked by two SSR markers, ORS1197-2 and ORS653a, at 3.3 and 1.8 cM of genetic distance, respectively. The markers for R (5) developed in this study will provide a useful tool for speeding up deployment of the R (5) gene in commercial sunflower hybrid production. C1 [Qi, L. L.; Gulya, T. J.; Hulke, B. S.; Vick, B. A.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. RP Qi, LL (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU USDA-ARS CRIS [5442-21000-034-00D] FX We thank Drs. Shiaoman Chao and Gerald Seiler for critical review of the manuscript, and Angelia Hogness and Megan Ramsett for technical assistance. This project was supported by the USDA-ARS CRIS Project No. 5442-21000-034-00D. NR 53 TC 9 Z9 9 U1 1 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2012 VL 30 IS 2 BP 745 EP 756 DI 10.1007/s11032-011-9659-6 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 985MN UT WOS:000307270900011 ER PT J AU Pang, MX Percy, RG Hughs, SE Jones, DC Zhang, JF AF Pang, M. X. Percy, R. G. Hughs, S. E. Jones, D. C. Zhang, J. F. TI Identification of genes that were differentially expressed and associated with fiber yield and quality using cDNA-AFLP and a backcross inbred line population SO MOLECULAR BREEDING LA English DT Article DE Cotton; cDNA-AFLP; Backcross inbred lines; Sequence-tagged sites ID GOSSYPIUM-BARBADENSE COTTON; SUCROSE SYNTHASE EXPRESSION; HOT BORATE METHOD; MOLECULAR DISSECTION; SELF APPROACH; QTL ANALYSIS; HIRSUTUM L.; INTERSPECIFIC VARIATION; TETRAPLOID COTTON; CELL INITIATION AB Numerous quantitative trait loci (QTLs) have been identified from segregating hybrid populations between Upland (Gossypium hirsutum) and Pima (G. barbadense) cotton. However, the genetic basis of these QTLs is currently unknown. In the present study, an interspecific backcross inbred line (BIL) population was tested at two locations in 2005 and 2006, and used to identify and isolate polymorphic transcript-derived fragments (TDFs) based on complementary DNA (cDNA)-amplified fragment length polymorphism (AFLP) analysis. The BILs were developed from a cross between Upland SG 747 as the recurrent parent and Pima S-7, followed by two generations of backcrossing and three generations of selfing. Of a total of 675 cDNA-AFLP fragments amplified from 15 primer combinations, 143 loci showed qualitative differences (TDFs) among BILs, and 44 TDF loci were significantly correlated with fiber yield and quality traits. Of the more than half of the TDFs cloned and sequenced, 47.4% had unknown function and many were converted to sequence tagged site (STS) markers. The identification of indels was informative in resolving homologous sequences when comparing cDNA-AFLP libraries from the same primer combination among Upland SG 747, Acala 1517-99, and Pima Phy 76. Differential expression of selected subset of TDFs associated with fiber traits was detected by reverse transcription-polymerase chain reaction (RT-PCR) using ovaries of eight selected BILs at 10 days post anthesis (DPA), and SG 747 and Pima 76 at 0, 4, 8, and 13 days. Quantitative RT-PCR was further used for validation on five TDFs using ovaries of Acala 1517-99 and Pima Phy 76 from the four early boll developmental stages. The present study has identified a number of genes whose expression was associated with fiber yield and quality traits. C1 [Pang, M. X.; Zhang, J. F.] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Percy, R. G.] USDA ARS, College Stn, TX 77845 USA. [Hughs, S. E.] USDA ARS, SW Cotton Ginning Res Lab, Mesilla Pk, NM 88003 USA. [Jones, D. C.] Cotton Inc, Cary, NC 27513 USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM jinzhang@nmsu.edu FU USDA-ARS; Cotton Incorporated; New Mexico Agricultural Experiment Station FX This work was supported by USDA-ARS, Cotton Incorporated, and New Mexico Agricultural Experiment Station. NR 56 TC 6 Z9 6 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2012 VL 30 IS 2 BP 975 EP 985 DI 10.1007/s11032-011-9682-7 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 985MN UT WOS:000307270900029 ER PT J AU Pang, MX Percy, RG Stewart, JM Hughs, E Zhang, JF AF Pang, Mingxiong Percy, R. G. Stewart, J. McD Hughs, E. Zhang, Jinfa TI Comparative transcriptome analysis of Pima and Acala cotton during boll development using 454 pyrosequencing technology SO MOLECULAR BREEDING LA English DT Article DE Cotton; Boll development; Transcriptome; Pyrosequencing ID HOT BORATE METHOD; F-SP VASINFECTUM; GENE-EXPRESSION; ALLOTETRAPLOID COTTON; GOSSYPIUM-HIRSUTUM; FIBER DEVELOPMENT; CELL ELONGATION; FIELD PERFORMANCE; SNP DISCOVERY; INFECTION AB Information on gene expression during cotton fiber development for high fiber quality genotypes including Pima (Gossypium barbadense L.) and Acala cotton (G. hirsutum L.) is lacking, impeding the discovery of novel genes and DNA markers for fiber quality traits. In the present study, massively parallel sequencing technology was used to generate a total of 394,301 expressed sequence tag (EST) sequence reads, totaling over 42 million bp, from immature ovaries at 10 days post-anthesis of Pima Phy 76 and Acala 1517-99. For Pima Phy 76, 1,900 EST contigs were tentatively assembled from 21.8% (50,876) ESTs which resulted in 1,541 tentative consensus sequences (TCs). 1,005 and 1,064 EST contigs were homologous to TCs from the A(2) and D-5 genomes, respectively, while 738 of the EST contigs were shared by both genomes. For Acala 1517-99, 2,151 EST contigs were tentatively assembled from 24.5% (39,368) ESTs and resulted in 1,787 TCs. 1,220 and 1,206 EST contigs were homologous to known transcripts from A(2) and D-5 genomes and only 452 of the EST contigs were shared by both genomes. 691 EST contigs are also homologous (E-value a parts per thousand currency sign 1 x 10(-10)) between Pima Phy 76 and Acala 1517-99, of which 38 contigs pairs were highly homologous (E-value a parts per thousand currency sign 1 x 10(-60)) with sequence variations, predominantly single nucleotide polymorphisms (SNPs). A Blast search of a sample of contigs to Upland cotton ESTs in GenBank indicated that more than 75% EST contigs may contain SNPs. This report represents the first research in cotton using 454 pyrosequencing technology to enrich ESTs for high fiber quality cotton. C1 [Pang, Mingxiong; Zhang, Jinfa] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Percy, R. G.] USDA ARS, SPARC, Crop Germplasm Res Unit, College Stn, TX 77845 USA. [Stewart, J. McD] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Hughs, E.] USDA ARS, SW Cotton Ginning Res Lab, Mesilla Pk, NM 88003 USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM jinzhang@nmsu.edu FU USDA-ARS; Cotton Incorporated; New Mexico Agricultural Experiment Station FX The research reported in this paper was in part funded by USDA-ARS, Cotton Incorporated, and New Mexico Agricultural Experiment Station. NR 56 TC 6 Z9 7 U1 0 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2012 VL 30 IS 2 BP 1143 EP 1153 DI 10.1007/s11032-012-9702-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 985MN UT WOS:000307270900044 ER PT J AU Wang, XZ Jiang, GL Green, M Scott, RA Hyten, DL Cregan, PB AF Wang, Xianzhi Jiang, Guo-Liang Green, Marci Scott, Roy A. Hyten, David L. Cregan, Perry B. TI Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean SO MOLECULAR BREEDING LA English DT Article DE Soybean; Palmitic acid; Stearic acid; QTL; Marker-assisted selection ID SUDDEN-DEATH SYNDROME; GENETIC-LINKAGE MAP; PALMITIC ACID; SEED OIL; FIELD-RESISTANCE; QTL; INHERITANCE; GERMPLASM; SELECTION; PERCENTAGE AB Soybean [Glycine max (L.) Merr.] is an important crop which contributes approximately 58% of the world's oilseed production. Palmitic and stearic acids are the two main saturated fatty acids in soybean oil. Different levels of saturated fatty acids are desired depending on the uses of the soybean oil. Vegetable oil low in saturated fatty acids is preferred for human consumption, while for industrial applications, soybean oil with higher levels of saturated fatty acids is more suitable. The objectives of this study were to identify quantitative trait loci (QTL) for saturated fatty acids, analyze the genetic effects of single QTL and QTL combinations, and discuss the potential of marker-assisted selection in soybean breeding for modified saturated fatty acid profiles. A population of recombinant inbred lines derived from the cross of SD02-4-59 x A02-381100 was grown in five environments and the seed samples from each environment were evaluated for fatty acid content. Genotyping of the population was performed with 516 polymorphic single nucleotide polymorphism markers and 298 polymorphic simple sequence repeat markers. Eight QTL for palmitic acid, five QTL for stearic acid and nine QTL for total saturated fatty acids were detected by composite interval mapping and/or interval mapping, with a high level of consistency or repeatability in multiple environments. Most of these QTL have not been reported previously, with the exception of qPAL-A1 which confirmed the result of a previous study. Significant QTL x QTL interactions were not detected. However, significant QTL x environment interactions were detected in most cases. Comparisons of two-locus and three-locus combinations indicated that cumulative effects of QTL were significant for both palmitic and stearic acids. QTL pyramiding by molecular marker-assisted selection would be an appropriate strategy for improvement of saturated fatty acids in soybean. C1 [Wang, Xianzhi; Jiang, Guo-Liang; Green, Marci] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Scott, Roy A.] USDA ARS, GWCC BLTSVL, Beltsville, MD 20705 USA. [Hyten, David L.; Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Jiang, GL (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM guo-liang.jiang@sdstate.edu OI Hyten, David/0000-0001-6324-9389 FU USB Seed Composition Research project via USDA/ARS; South Dakota Soybean Research & Promotion Council project; USDA-NIFA Hatch project [SD00H374-10]; South Dakota Agricultural Experiment Station FX This research was supported in part by USB Seed Composition Research project via USDA/ARS, South Dakota Soybean Research & Promotion Council project, USDA-NIFA Hatch project (SD00H374-10), and South Dakota Agricultural Experiment Station. The determination of fatty acids was completed in the USDA/ARS lab in Peoria, IL and the Iowa State University DNA Facility in Ames, IA. The parental line (A02-381100) was provided by Dr. Walter Fehr, Iowa State University. NR 53 TC 10 Z9 15 U1 3 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2012 VL 30 IS 2 BP 1163 EP 1179 DI 10.1007/s11032-012-9704-0 PG 17 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 985MN UT WOS:000307270900046 ER PT J AU Mott, IW Wang, RRC AF Mott, I. W. Wang, R. R. -C. TI Genetic variation among laboratory accessions of Chinese Spring wheat (Triticum aestivum L.) SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE AFLP; Chinese Spring; salt tolerance; wheat ID DISOMIC ADDITION LINES; THINOPYRUM-JUNCEUM; GERMPLASM LINES; TOLERANCE AB Chinese Spring (CS) wheat (Triticum aestivum L.) is commonly used in genetic research including cytogenetic analysis, molecular mapping and germplasm development. Aneuploid lines of alien chromosomes in the CS background have been used in studies with diverse objectives. Thousands of genomic and complementary DNA sequences from expressed sequence tag (EST) libraries of biotic- and abiotic-stressed tissues are publicly available from CS. Gene expression analysis of salt-tolerant wheat lines, W4909 and W4910, compared with the CS common wheat background led to the discovery of several expressed sequences that were absent in the CS accession held in our laboratory. A survey of 20 CS accessions from 13 laboratories using the polymerase chain reaction with gene-specific primers for eight salt-responsive genes resulted in amplification success ranging from 15 to 100%. Amplified fragment length polymorphism analysis showed that 99% of the genetic variation was among the accessions while the remaining 1% was within the accessions. A neighbour-joining phylogram showed that four of the five CS accessions from the International Maize and Wheat Improvement Center (CIMMYT) grouped with the salt-tolerant wheat cultivar Yecora Rojo while the remaining 16 CS accessions had limited genetic differences. Thus, variation exists among these highly self-pollinating CS sources, suggesting that appropriate consideration should be taken when using CS accessions to conduct molecular and genetic analyses. C1 [Mott, I. W.; Wang, R. R. -C.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. RP Mott, IW (reprint author), USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. EM ivan.mott@ars.usda.gov NR 11 TC 1 Z9 1 U1 0 U2 4 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD AUG PY 2012 VL 10 IS 2 BP 97 EP 100 DI 10.1017/S1479262112000068 PG 4 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA 984JR UT WOS:000307186400001 ER PT J AU Harnly, J AF Harnly, J. TI Chemical fingerprinting for identification/authentication of botanical materials SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Harnly, J.] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1031 EP 1031 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800012 ER PT J AU Kinghorn, AD Powell, RG Ferreira, D Proteau, PJ Pearce, CJ Cardellina, JH AF Kinghorn, A. D. Powell, R. G. Ferreira, D. Proteau, P. J. Pearce, C. J. Cardellina, J. H., II TI Lloydia and the Journal of Natural Products: 75 years of publication SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Kinghorn, A. D.] Ohio State Univ, Coll Pharm, Columbus, OH 43210 USA. [Powell, R. G.] USDA, Natl Ctr Agr Utilizat & Res, Peoria, IL 61604 USA. [Ferreira, D.] Univ Mississippi, Sch Pharm, University, MS 38677 USA. [Proteau, P. J.] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. [Pearce, C. J.] Mycosynthetix Inc, Hillsborough, NC 27278 USA. [Cardellina, J. H., II] McCormick & Co Inc, Tech Innovat Ctr, Hunt Valley, MD 21031 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1032 EP 1032 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800018 ER PT J AU Cardellina, JH Roxas-Duncan, VI Montgomery, V Eccard, V Campbell, Y Hu, X Tawa, GJ Khavrutskii, I Wallqvist, A Gloer, JB Phatak, NL Holler, U Soman, AG Joshi, BK Hein, SM Wicklow, DT Smith, LA AF Cardellina, J. H., II Roxas-Duncan, V., I Montgomery, V Eccard, V Campbell, Y. Hu, X. Tawa, G. J. Khavrutskii, I Wallqvist, A. Gloer, J. B. Phatak, N. L. Hoeller, U. Soman, A. G. Joshi, B. K. Hein, S. M. Wicklow, D. T. Smith, L. A. TI Fungal BIS-Naphthopyrones as inhibitors of botulinum neurotoxin serotype A SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Cardellina, J. H., II; Roxas-Duncan, V., I; Montgomery, V; Eccard, V; Campbell, Y.] USA, Div Integrated Toxicol, Med Res Inst Infect Dis, Frederick, MD USA. [Hu, X.; Tawa, G. J.; Khavrutskii, I; Wallqvist, A.] USA, Biotechnol High Performance Comp Software Applica, Telemed & Adv Technol Res Ctr, Med Res & Mat Command, Frederick, MD USA. [Gloer, J. B.; Phatak, N. L.; Hoeller, U.; Soman, A. G.; Joshi, B. K.; Hein, S. M.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1044 EP 1045 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800052 ER PT J AU Stavropoulou, MI Aligiannis, N Kalpoutzakis, E Angelis, A Duke, SO Fokialakis, N Mitakou, S AF Stavropoulou, M., I Aligiannis, N. Kalpoutzakis, E. Angelis, A. Duke, S. O. Fokialakis, N. Mitakou, S. TI Screeninig for phytotoxicity crude exctracts from the flora of crete and phytochemical investigation of Bellis longifolia SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Stavropoulou, M., I; Aligiannis, N.; Kalpoutzakis, E.; Angelis, A.; Fokialakis, N.; Mitakou, S.] Univ Athens, Dept Pharmacognosy & Nat Prod Chem, Fac Pharm, GR-15771 Athens, Greece. [Duke, S. O.] Natl Ctr Nat Prod Res, Nat Prod Utilizat Res Unit, USDA, ARS, University, MS 38677 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1105 EP 1105 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800320 ER PT J AU Sun, J Chen, P AF Sun, J. Chen, P. TI UHPLC/HRMS analysis of African mango (Irvingia gabonensis) seeds, seed extracts, and African mango based dietary supplements SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Sun, J.; Chen, P.] Agr Res Serv, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1119 EP 1119 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800386 ER PT J AU Leyte-Lugo, M Cerda-Garcia-Rojas, CM Gonzalez, MD Glenn, A Mata, R AF Leyte-Lugo, M. Cerda-Garcia-Rojas, C. M. del Carmen Gonzalez, M. Glenn, A. Mata, R. TI 9S,11R-(+)-Ascosalitoxin from an endophytic fungus isolated from Hintonia latiflora SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Leyte-Lugo, M.; Mata, R.] Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico. [Cerda-Garcia-Rojas, C. M.] Inst Politecn Nacl, Dept Quim, Ctr Invest & Estudios Avanzados, Mexico City 07000, DF, Mexico. [del Carmen Gonzalez, M.] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico. [Glenn, A.] ARS, USDA, Toxicol & Mycotoxin Res Unit, Athens, GA USA. RI Mata, Rachel/I-4435-2014 OI Mata, Rachel/0000-0002-2861-2768 NR 0 TC 0 Z9 0 U1 1 U2 3 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1157 EP 1157 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042800557 ER PT J AU Kaur, A Jordan, AM Baltrusaitis, J Swenson, DC Wicklow, DT Gloer, JB AF Kaur, A. Jordan, A. M. Baltrusaitis, J. Swenson, D. C. Wicklow, D. T. Gloer, J. B. TI New phomactin analogues from an unidentified fungicolous fungal isolate obtained from the surface of a polypore SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Kaur, A.; Jordan, A. M.; Baltrusaitis, J.; Swenson, D. C.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RI Baltrusaitis, Jonas/F-9367-2013 NR 0 TC 0 Z9 0 U1 0 U2 2 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1162 EP 1162 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801016 ER PT J AU Whiteman, SA Dowd, PF Wicklow, DT Gloer, JB AF Whiteman, S. A. Dowd, P. F. Wicklow, D. T. Gloer, J. B. TI Kipukasin and oxepinamide derivatives from an undescribed Aspergillus sp SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Whiteman, S. A.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Dowd, P. F.; Wicklow, D. T.] ARS, Mycotoxin Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1162 EP 1163 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801020 ER PT J AU Cannistra, JC Wicklow, DT Gloer, JB AF Cannistra, J. C. Wicklow, D. T. Gloer, J. B. TI New lapatin analogues from a fungicolous isolate of Aspergillus sp SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Cannistra, J. C.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1165 EP 1166 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801034 ER PT J AU Phatak, NL Wicklow, DT Gloer, JB AF Phatak, N. L. Wicklow, D. T. Gloer, J. B. TI A new cyclic depsipeptide from a fungicolous Hawaiian Isolate of Phaeoacremonium sp SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Phatak, N. L.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] Natl Ctr Agr Utilizat Res, Mycotoxin Res Unit, ARS, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1165 EP 1165 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801030 ER PT J AU Wu, SB Meyer, RS Whitaker, BD Litt, A Kennelly, E AF Wu, S. B. Meyer, R. S. Whitaker, B. D. Litt, A. Kennelly, E. TI New glucosylated caffeoylquinic acid derivatives in the invasive soda apple, Solanum viarum L SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Wu, S. B.; Meyer, R. S.; Kennelly, E.] CUNY, Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA. [Wu, S. B.; Meyer, R. S.; Kennelly, E.] CUNY, Grad Ctr, Bronx, NY 10468 USA. [Meyer, R. S.; Litt, A.] New York Bot Garden, Bronx, NY 10458 USA. [Whitaker, B. D.] ARS, Food Qual Lab, Beltsville Agr Res Ctr W, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1200 EP 1200 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801187 ER PT J AU Lee, ST Panter, KE Gardner, DR Green, BT Welch, KD Zhang, J Chang, CWT AF Lee, S. T. Panter, K. E. Gardner, D. R. Green, B. T. Welch, K. D. Zhang, J. Chang, C. W. T. TI A monoclonal antibody-based elisa for the hedgehog inhibitors cyclopamine and cyclopamine-KAAD SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Lee, S. T.; Panter, K. E.; Gardner, D. R.; Green, B. T.; Welch, K. D.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Zhang, J.; Chang, C. W. T.] Utah State Univ, Dept Chem & Biochem, Logan, UT 84322 USA. RI zhang, jianjun/H-7529-2012 NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1257 EP 1258 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801453 ER PT J AU Gozlekci, S Balci, F Ayala-Silva, T Kalinkara, EC AF Gozlekci, S. Balci, F. Ayala-Silva, T. Kalinkara, E. C. TI Some physical and chemical changes of pomegranate fruit characteristics during storage shelf-life conditions SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Gozlekci, S.; Kalinkara, E. C.] Akdeniz Univ, Fac Agr, Dept Hort, TR-07058 Antalya, Turkey. [Balci, F.] Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey. [Ayala-Silva, T.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1278 EP 1278 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801547 ER PT J AU Meepagala, KM Bernier, U Burandt, C Duke, SO AF Meepagala, K. M. Bernier, U. Burandt, C. Duke, S. O. TI Natural products from plants and their synthetic analogs against pests SO PLANTA MEDICA LA English DT Meeting Abstract CT International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF CY JUL 28-AUG 01, 2012 CL New York, NY SP Amer Soc Pharmacognosy (ASP), Soc Med Plant & Nat Prod Res (GA), Italian Soc Phytochem (SIF), Phytochem Soc Europe (PSE), French Speaking Soc Pharmacognosy (AFERP) C1 [Meepagala, K. M.; Duke, S. O.] USDA ARS, NPURU, University, MS 38677 USA. [Bernier, U.] USDA ARS CMAVE, Mosquito & Fly Res Unit, Gainesville, FL 32608 USA. [Burandt, C.] Univ Mississippi, NCNPR, University, MS 38677 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2012 VL 78 IS 11 BP 1292 EP 1292 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 982KT UT WOS:000307042801612 ER PT J AU Blomberg, LA Telugu, BPVL AF Blomberg, L. A. Telugu, B. P. V. L. TI Twenty Years of Embryonic Stem Cell Research in Farm Animals SO REPRODUCTION IN DOMESTIC ANIMALS LA English DT Article ID LEUKEMIA INHIBITORY FACTOR; IN-VITRO; BOVINE EMBRYOS; MOUSE EMBRYOS; SELF-RENEWAL; PIG EMBRYOS; LINES; PORCINE; GENERATION; PLURIPOTENCY AB Notable distinctions between an embryonic stem cell (ESC) and somatic cell are that an ESC can maintain an undifferentiated state indefinitely, self-renew, and is pluripotent, meaning that the ESC can potentially generate cells representing all the three primordial germ layers and contribute to the terminally differentiated cells of a conceptus. These attributes make the ESC an ideal source for genome editing for both agricultural and biomedical applications. Although, ESC lines have been successfully established from rodents and primates, authentic ungulate stem cell lines on the contrary are still not available. Outstanding issues including but not limited to differences in pluripotency characteristics among the existing ESC lines, pre-implantation embryo development, pluripotency pathways, and culture conditions plague our efforts to establish authentic ESC lines from farm animals. In this review, we highlight some of these issues and discuss how the recent derivation of induced pluripotent stem cells (iPSCs) might augur the establishment of robust authentic ESC lines from farm animals. C1 [Blomberg, L. A.] ARS, Anim Biosci & Biotechnol Lab, USDA, Anim & Nat Resources Inst, Beltsville, MD 20705 USA. [Telugu, B. P. V. L.] Univ Maryland Coll Pk, Dept Anim & Avian Sci, College Pk, MD USA. RP Blomberg, LA (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Anim & Nat Resources Inst, Bldg 200,Rm 217A,BARC E, Beltsville, MD 20705 USA. EM leann.blomberg@ars.usda.gov NR 59 TC 13 Z9 18 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0936-6768 J9 REPROD DOMEST ANIM JI Reprod. Domest. Anim. PD AUG PY 2012 VL 47 SU 4 SI SI BP 80 EP 85 DI 10.1111/j.1439-0531.2012.02059.x PG 6 WC Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences SC Agriculture; Reproductive Biology; Veterinary Sciences GA 980UG UT WOS:000306918700013 PM 22827354 ER PT J AU Blackburn, HD AF Blackburn, H. D. TI Genetic Selection and Conservation of Genetic Diversity SO REPRODUCTION IN DOMESTIC ANIMALS LA English DT Article ID CATTLE; RESOURCES; BREEDS AB For 100s of years, livestock producers have employed various types of selection to alter livestock populations. Current selection strategies are little different, except our technologies for selection have become more powerful. Genetic resources at the breed level have been in and out of favour over time. These resources are the raw materials used to manipulate populations, and therefore, they are critical to the past and future success of the livestock sector. With increasing ability to rapidly change genetic composition of livestock populations, the conservation of these genetic resources becomes more critical. Globally, awareness of the need to steward genetic resources has increased. A growing number of countries have embarked on large scale conservation efforts by using in situ, ex situ (gene banking), or both approaches. Gene banking efforts have substantially increased and data suggest that gene banks are successfully capturing genetic diversity for research or industry use. It is also noteworthy that both industry and the research community are utilizing gene bank holdings. As pressures grow to meet consumer demands and potential changes in production systems, the linkage between selection goals and genetic conservation will increase as a mechanism to facilitate continued livestock sector development. C1 ARS, Natl Anim Germplasm Programme, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. RP Blackburn, HD (reprint author), ARS, Natl Anim Germplasm Programme, Natl Ctr Genet Resources Preservat, USDA, 1111 S Mason St, Ft Collins, CO 80521 USA. EM harvey.blackburn@ars.usda.gov NR 36 TC 3 Z9 5 U1 0 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0936-6768 J9 REPROD DOMEST ANIM JI Reprod. Domest. Anim. PD AUG PY 2012 VL 47 SU 4 SI SI BP 249 EP 254 DI 10.1111/j.1439-0531.2012.02083.x PG 6 WC Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences SC Agriculture; Reproductive Biology; Veterinary Sciences GA 980UG UT WOS:000306918700037 PM 22827378 ER PT J AU Tarvis, KM Purdy, PH Graham, JK AF Tarvis, K. M. Purdy, P. H. Graham, J. K. TI Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster sperm SO REPRODUCTION IN DOMESTIC ANIMALS LA English DT Meeting Abstract CT 17th International Congress on Animal Reproduction (ICAR) CY JUL 29-AUG 02, 2012 CL Vancouver, CANADA SP Bioniche, Merial, Sanofi Co, Pfizer Anim Hlth, MSD Anim Hlth, Imv Technol, Minitube, Semex, Canadian Food Inspect Agcy DE Poultry; sperm; cryoprotectants; cryopreservation C1 [Tarvis, K. M.; Graham, J. K.] Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA. [Purdy, P. H.] ARS, USDA, NCGRP, Natl Anim Germplasm Program, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0936-6768 J9 REPROD DOMEST ANIM JI Reprod. Domest. Anim. PD AUG PY 2012 VL 47 SU 4 SI SI BP 446 EP 446 PG 1 WC Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences SC Agriculture; Reproductive Biology; Veterinary Sciences GA 980UG UT WOS:000306918700142 ER PT J AU Minten, MA Bilby, TR Bruno, RGS Allen, CC Roberts, CA Wang, Z Sawyer, JE Tibary, A Neibergs, HL Geary, TW Spencer, TE AF Minten, M. A. Bilby, T. R. Bruno, R. G. S. Allen, C. C. Roberts, C. A. Wang, Z. Sawyer, J. E. Tibary, A. Neibergs, H. L. Geary, T. W. Spencer, T. E. TI Identification and analysis of beef heifers with superior capacity for fertility SO REPRODUCTION IN DOMESTIC ANIMALS LA English DT Meeting Abstract CT 17th International Congress on Animal Reproduction (ICAR) CY JUL 29-AUG 02, 2012 CL Vancouver, CANADA SP Bioniche, Merial, Sanofi Co, Pfizer Anim Hlth, MSD Anim Hlth, Imv Technol, Minitube, Semex, Canadian Food Inspect Agcy DE Early pregnancy; early embryonic loss; cattle; SNP C1 [Minten, M. A.; Allen, C. C.; Wang, Z.; Neibergs, H. L.; Spencer, T. E.] Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA. [Bilby, T. R.] Texas AgriLife Res & Extens, Stephenville, TX USA. [Bruno, R. G. S.] W Texas A&M Univ, Amarillo, TX USA. [Bruno, R. G. S.] Texas AgriLife Res & Extens, Amarillo, TX USA. [Roberts, C. A.; Geary, T. W.] USDA ARS, Miles City, MT USA. [Sawyer, J. E.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Tibary, A.] Washington State Univ, Coll Vet Med, Dept Vet Clin Sci, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0936-6768 J9 REPROD DOMEST ANIM JI Reprod. Domest. Anim. PD AUG PY 2012 VL 47 SU 4 SI SI BP 503 EP 503 PG 1 WC Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences SC Agriculture; Reproductive Biology; Veterinary Sciences GA 980UG UT WOS:000306918700300 ER PT J AU Xu, TS Xu, T Lan, YB Wu, WF Zhang, HH Zhu, H AF Xu Tian-shuang Xu Tao Lan Yu-bin Wu Wen-fu Zhang Hui-hui Zhu Hang TI Study on a Method for Fast Selecting Feature Wavelengths from the Spectral Information of Crop Nitrogen SO SPECTROSCOPY AND SPECTRAL ANALYSIS LA Chinese DT Article DE Particle swarm optimization (PSO); Wavelength selection; Uniform design; Nitrogen content; Spectroscopy AB Research on a method for fast selecting feature wavelengths from the nitrogen spectral information is necessary, which can determine the nitrogen content of crops. Based on the uniformity of uniform design, the present paper proposed an improved particle swarm optimization (PSO) method. The method can choose the initial particle swarm uniformly and describe the optimization space well by fewer sample points, which is helpful to avoiding the local optimum and accelerate the convergence. Then, the method was applied to fast select the nitrogen spectral wavelengths of soybean, cotton and maize. Calibration models based on the partial least square (PLS) method and selected wavelengths were constructed. The results illustrate that compared with the original wavelengths, the number of selected wavelengths decreases about 93%, which means the computation is simplified. Also, the precision of PLS prediction mode based on the selected wavelengths improves by 34% at least, and the prediction ability of calibration model increases greatly. Therefore, the proposed method is both correct and effective. C1 [Xu Tian-shuang; Xu Tao; Zhu Hang] Jilin Univ, Coll Mech Sci & Engn, Changchun 130022, Peoples R China. [Lan Yu-bin] USDA ARS, College Stn, TX 77845 USA. [Wu Wen-fu] Jilin Univ, Coll Biol & Agr Engn, Changchun 130022, Peoples R China. [Zhang Hui-hui] USDA ARS, Parlier, CA 93648 USA. RP Zhu, H (reprint author), Jilin Univ, Coll Mech Sci & Engn, Changchun 130022, Peoples R China. EM tsxu0214@163.com; hangzhu@jlu.edu.cn NR 12 TC 0 Z9 1 U1 1 U2 6 PU OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS PI BEIJING PA NO 76 COLLAGE SOUTH RD BEIJING, BEIJING 100081, PEOPLES R CHINA SN 1000-0593 J9 SPECTROSC SPECT ANAL JI Spectrosc. Spectr. Anal. PD AUG PY 2012 VL 32 IS 8 BP 2185 EP 2189 DI 10.3964/j.issn.1000-0593(2012)08-2185-05 PG 5 WC Spectroscopy SC Spectroscopy GA 983NW UT WOS:000307127000035 ER PT J AU Carrillo, Y Dijkstra, FA Pendall, E Morgan, JA Blumenthal, DM AF Carrillo, Yolima Dijkstra, Feike A. Pendall, Elise Morgan, Jack A. Blumenthal, Dana M. TI Controls over Soil Nitrogen Pools in a Semiarid Grassland Under Elevated CO2 and Warming SO ECOSYSTEMS LA English DT Article DE elevated CO2; warming; grasslands; climate change; nitrogen; dissolved organic nitrogen; nitrate; ammonium; dissolved organic carbon; moisture; temperature; C-13 ID CARBON-DIOXIDE ENRICHMENT; PLANT-GROWTH; ECOSYSTEM RESPONSE; N-MINERALIZATION; ATMOSPHERIC CO2; INORGANIC N; GAS FLUXES; TEMPERATURE; AVAILABILITY; METAANALYSIS AB Long-term responses of terrestrial ecosystems to the combined effects of warming and elevated CO2 (eCO(2)) will likely be regulated by N availability. The stock of soil N determines availability for organisms, but also influences loss to the atmosphere or groundwater. eCO(2) and warming can elicit changes in soil N via direct effects on microbial and plant activity, or indirectly, via soil moisture. Detangling the interplay of direct- and moisture-mediated impacts on soil N and the role of organisms in controlling soil N will improve predictions of ecosystem-level responses. We followed individual soil N pools over two growing seasons in a semiarid temperate grassland, at the Prairie Heating and CO2 Enrichment experiment. We evaluated relationships of N pools with environmental factors and explored the role of plants by assessing plant biomass, plant N, and plant inputs to soil. We also assessed N forms in plots with and without vegetation to remove plant-mediated effects. Our study demonstrated that the effects of warming and eCO(2) are highly dependent on individual N form and on year. In this water-constrained grassland, eCO(2), warming and their combination appear to impact soil N pools through a complex combination of direct- and moisture-mediated effects. eCO(2) decreased NO3 (-) but had neutral to positive effects on NH4 (+) and dissolved organic N (DON), particularly in a wet year. Warming increased NO3 (-) availability due to a combination of indirect drying and direct temperature-driven effects. Warming also increased DON only in vegetated plots, suggesting plant mediation. Our results suggest that impacts of combined eCO(2) and warming are not always equivalent for plant and soil pools; although warming can help offset the decrease in NO3 (-) availability for plants under eCO(2), the NO3 (-) pool in soil is mainly driven by the negative effects of eCO(2). C1 [Carrillo, Yolima] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Carrillo, Yolima; Dijkstra, Feike A.] Univ Sydney, Dept Environm Sci, Fac Agr & Environm, Eveleigh, NSW 2015, Australia. [Pendall, Elise] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Pendall, Elise] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Morgan, Jack A.; Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, Crops Res Lab, Ft Collins, CO 80526 USA. RP Carrillo, Y (reprint author), Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. EM yolima.carrillo@sydney.edu.au RI Dijkstra, Feike/H-2182-2012; Blumenthal, Dana/J-3106-2012; OI Pendall, Elise/0000-0002-1651-8969; Dijkstra, Feike/0000-0002-6191-6018 FU USDA-CSREES Soil Processes Program [2008-35107-18655]; US Department of Energy's Office of Science (BER) through the Terrestrial Ecosystem Science program; Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University, by NSF [1021559]; Australian Research Council [FT100100779] FX We thank Dan LeCain, David Smith, Erik Hardy, and Matthew Parsons for their technical assistance and Joanne Newcomb, Megan Steinweg, Hannah Munn, Courtney Ellis, Christine Rumsey, and Jennifer Bell, for assistance in the field and in the laboratory. This project was supported by a USDA-CSREES Soil Processes Program (Grant no. 2008-35107-18655), by the US Department of Energy's Office of Science (BER) through the Terrestrial Ecosystem Science program and the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University, by NSF (DEB# 1021559), and by the Australian Research Council (FT100100779). NR 56 TC 20 Z9 23 U1 7 U2 76 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 J9 ECOSYSTEMS JI Ecosystems PD AUG PY 2012 VL 15 IS 5 BP 761 EP 774 DI 10.1007/s10021-012-9544-0 PG 14 WC Ecology SC Environmental Sciences & Ecology GA 981FN UT WOS:000306952400006 ER PT J AU Mayor, JR Schuur, EAG Mack, MC Hollingsworth, TN Baath, E AF Mayor, Jordan R. Schuur, Edward A. G. Mack, Michelle C. Hollingsworth, Terresa N. Baath, Erland TI Nitrogen Isotope Patterns in Alaskan Black Spruce Reflect Organic Nitrogen Sources and the Activity of Ectomycorrhizal Fungi SO ECOSYSTEMS LA English DT Article DE 15N; black spruce; denitrifier method; dissolved organic nitrogen; ectomycorrhiza; isotope fractionation; mixing models ID NATURAL N-15 ABUNDANCE; ECOSYSTEM CARBON STORAGE; FATTY-ACID ANALYSIS; MYCORRHIZAL FUNGI; BOREAL FOREST; ARCTIC TUNDRA; MICROBIAL BIOMASS; SOIL N; AGRICULTURAL ABANDONMENT; SPECIES COMPOSITION AB Global patterns in soil, plant, and fungal stable isotopes of N (delta N-15) show promise as integrated metrics of N cycling, particularly the activity of ectomycorrhizal (ECM) fungi. At small spatial scales, however, it remains difficult to differentiate the underlying causes of plant delta N-15 variability and this limits the application of such measurements to better understand N cycling. We conducted a landscape-scale analysis of delta N-15 values from 31 putatively N-limited monospecific black spruce (Picea mariana) stands in central Alaska to assess the two main hypothesized sources of plant delta N-15 variation: differing sources and ECM fractionation. We found roughly 20% of the variability in black spruce foliar N and delta N-15 values to be correlated with the concentration and delta N-15 values of soil NH4 (+) and dissolved organic N (DON) pools, respectively. However, N-15-based mixing models from 24 of the stands suggested that fractionation by ECM fungi obscures the N-15 signature of soil N pools. Models, regressions, and N abundance data all suggested that increasing dependence on soil DON to meet black spruce growth demands predicates increasing reliance on ECM-derived N and that black spruce, on average, received 53% of its N from ECM fungi. Future research should partition the delta N-15 values within the soil DON pool to determine how choice of soil delta N-15 values influence modeled ECM activity. The C balance of boreal forests is tightly linked to N cycling and delta N-15 values may be useful metrics of changes to these connections. C1 [Mayor, Jordan R.; Schuur, Edward A. G.; Mack, Michelle C.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Mayor, Jordan R.] Smithsonian Trop Res Inst, Panama City 084303092, Panama. [Hollingsworth, Terresa N.] Univ Alaska Fairbanks, USDA, US Forest Serv, Pacific NW Res Stn,Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Baath, Erland] Lund Univ, Dept Biol, Microbial Ecol Grp, S-22362 Lund, Sweden. RP Mayor, JR (reprint author), Univ Florida, Dept Biol, Gainesville, FL 32611 USA. EM clavulina@gmail.com OI Baath, Erland/0000-0002-2616-1342 FU NSF [DGE-0221599]; Mycological Society of America; International Association of GeoChemistry Student Research Grant; Riewald-Olowo Graduate Research Award; University of Florida Graduate Student Council; DOE FX This study was partially supported by an NSF Doctoral Dissertation award (DGE-0221599), a Forest Fungal Ecology Research award of the Mycological Society of America, an International Association of GeoChemistry Student Research Grant, a Riewald-Olowo Graduate Research Award, and University of Florida Graduate Student Council Travel awards to JRM; DOE and NSF funding to EAGS; and, the logistical support offered by the Ecosystem Ecology Laboratory at the University of Alaska, Fairbanks and the Bonanza Creek Long-Term Ecological Research site. Martin Lavoie, Emily Tissier, Dominique Ardura, Rady Ho, Dat Nyguen, and Rachel Rubin provided field or laboratory assistance. Gary Laursen assisted with fungal identifications. The manuscript benefited from the input of John Hobbie and two anonymous reviewers. Raw data to be made available at the Bonanza Creek LTER database (http://www.lter.uaf.edu/data_b.cfm). NR 112 TC 17 Z9 18 U1 5 U2 90 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD AUG PY 2012 VL 15 IS 5 BP 819 EP 831 DI 10.1007/s10021-012-9548-9 PG 13 WC Ecology SC Environmental Sciences & Ecology GA 981FN UT WOS:000306952400010 ER PT J AU Butchko, RAE Brown, DW Busman, M Tudzynski, B Wiemann, P AF Butchko, Robert A. E. Brown, Daren W. Busman, Mark Tudzynski, Bettina Wiemann, Philipp TI Lae1 regulates expression of multiple secondary metabolite gene clusters in Fusarium verticillioides SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Fusarium verticillioides; Fumonisin; Secondary metabolism; LaeA ID FUJIKUROI MATING POPULATION; POLYKETIDE SYNTHASE GENES; HUMAN ESOPHAGEAL CANCER; RED PIGMENT BIKAVERIN; VELVET-LIKE COMPLEX; FUMONISIN BIOSYNTHESIS; ASPERGILLUS-NIDULANS; GIBBERELLA-MONILIFORMIS; MAIZE KERNELS; MYCOTOXINS AB The filamentous fungus Fusarium verticillioides can cause disease of maize and is capable of producing fumonisins, a family of toxic secondary metabolites linked to esophageal cancer and neural tube defects in humans and lung edema in swine and leukoencephalomalacia in equines. The expression of fumonisin biosynthetic genes is influenced by broad-domain transcription factors (global regulators) and Fum21, a pathway-specific transcription factor. LaeA is a global regulator that in Aspergillus nidulans, affects the expression of multiple secondary metabolite gene clusters by modifying heterochromatin structure. Here, we employed gene deletion analysis to assess the effect of loss of a F. verticillioides laeA orthologue, LAE1, on genome-wide gene expression and secondary metabolite production. Loss of Lae1 resulted in reduced expression of gene clusters responsible for synthesis of the secondary metabolites bikaverin, fumonisins, fusaric acid and fusarins as well as two clusters for which the corresponding secondary metabolite is unknown. Analysis of secondary metabolites revealed that, in contrast to a previously described Fusarium fujikuroi lae1 mutant, bikaverin production is reduced. Fumonisin production is unchanged in the F. verticillioides lae1 mutant. Complementation of the F. verticillioides mutant resulted in increased fumonisin production. (C) 2012 Elsevier Inc. All rights reserved. C1 [Butchko, Robert A. E.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, MWA,NCAUR, Peoria, IL 61604 USA. [Tudzynski, Bettina; Wiemann, Philipp] Univ Munster, Insitut Biol & Biotechnol Pflanzen, D-48143 Munster, Germany. RP Butchko, RAE (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, MWA,NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM rbutchko@aggienetwork.com NR 64 TC 42 Z9 44 U1 6 U2 44 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD AUG PY 2012 VL 49 IS 8 BP 602 EP 612 DI 10.1016/j.fgb.2012.06.003 PG 11 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 983VM UT WOS:000307146800002 PM 22713715 ER PT J AU Bolduc, N Yilmaz, A Mejia-Guerra, MK Morohashi, K O'Connor, D Grotewold, E Hake, S AF Bolduc, Nathalie Yilmaz, Alper Mejia-Guerra, Maria Katherine Morohashi, Kengo O'Connor, Devin Grotewold, Erich Hake, Sarah TI Unraveling the KNOTTED1 regulatory network in maize meristems SO GENES & DEVELOPMENT LA English DT Article DE KNOX transcription factors; chromatin immunoprecipitation; gene regulation; maize ID SHOOT APICAL MERISTEM; HOMEOBOX GENE; ARABIDOPSIS DEVELOPMENT; AUXIN TRANSPORT; EXPRESSION; TRANSCRIPTION; CYTOKININ; PROTEINS; MAINTENANCE; MUTATIONS AB KNOTTED1 (KN1)-like homeobox (KNOX) transcription factors function in plant meristems, self-renewing structures consisting of stem cells and their immediate daughters. We defined the KN1 cistrome in maize inflorescences and found that KN1 binds to several thousand loci, including 643 genes that are modulated in one or multiple tissues. These KN1 direct targets are strongly enriched for transcription factors (including other homeobox genes) and genes participating in hormonal pathways, most significantly auxin, demonstrating that KN1 plays a key role in orchestrating the upper levels of a hierarchical gene regulatory network that impacts plant meristem identity and function. C1 [Bolduc, Nathalie; O'Connor, Devin; Hake, Sarah] Univ Calif Berkeley, Ctr Plant Gene Express, USDA, ARS,Plant & Microbial Biol Dept, Berkeley, CA 94720 USA. [Yilmaz, Alper; Mejia-Guerra, Maria Katherine; Morohashi, Kengo; Grotewold, Erich] Ohio State Univ, Dept Mol Genet, Ctr Appl Plant Sci, Columbus, OH 43210 USA. RP Hake, S (reprint author), Univ Calif Berkeley, Ctr Plant Gene Express, USDA, ARS,Plant & Microbial Biol Dept, Berkeley, CA 94720 USA. EM hake@berkeley.edu RI Morohashi, Kengo/C-5511-2014; Yilmaz, Alper/C-7075-2014 OI Yilmaz, Alper/0000-0002-8827-4887 FU NSF [DBI-0604923, DBI-0701405, IOS-1125620]; ARS [5335-21000-013-00D]; NIH [5T32CA106196-05] FX We thank the Hake laboratory, J. Schnable, and A. Eveland for advice, discussion, and critical reading of the manuscript. The work was funded by NSF DBI-0604923 and ARS 5335-21000-013-00D to S.H., NSF DBI-0701405 and NSF IOS-1125620 to E.G., and NIH 5T32CA106196-05 to A.Y. NR 40 TC 75 Z9 83 U1 6 U2 39 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI COLD SPRING HARBOR PA 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA SN 0890-9369 J9 GENE DEV JI Genes Dev. PD AUG 1 PY 2012 VL 26 IS 15 BP 1685 EP 1690 DI 10.1101/gad.193433.112 PG 6 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA 984AO UT WOS:000307160000006 PM 22855831 ER PT J AU Edrington, TS Dowd, SE Farrow, RF Hagevoort, GR Callaway, TR Anderson, RC Nisbet, DJ AF Edrington, T. S. Dowd, S. E. Farrow, R. F. Hagevoort, G. R. Callaway, T. R. Anderson, R. C. Nisbet, D. J. TI Development of colonic microflora as assessed by pyrosequencing in dairy calves fed waste milk SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE bacterial diversity; Salmonella; waste milk; pasteurization ID ESCHERICHIA-COLI O157-H7; BACTERIAL DIVERSITY; MASTITIC MILK; MYCOBACTERIUM-PARATUBERCULOSIS; SALMONELLA; COLOSTRUM; MYCOPLASMA; CATTLE; FEED; COWS AB The objective of the current study was to examine the effect of pasteurization of waste milk, used to feed dairy calves, on the bacterial diversity of their lower gut. Using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing, fecal samples from dairy calves, ages 1 wk to 6 mo old and fed either pasteurized or nonpasteurized waste milk, were analyzed for bacterial diversity. Calves were maintained on 2 separate farms and, aside from how the waste milk was treated, were housed and cared for similarly. Fifteen calves were sampled from each age group (1, 2, and 4 wk, and 2, 4, and 6 mo of age; n = 90 samples per milk treatment, 180 total samples) on each farm via rectal palpation and the samples shipped and frozen before analysis. In general; bacterial diversity, as represented by the total number of different species, was greater for the calves fed pasteurized waste milk at all ages (except 1 wk of age) and increased with increasing age in both treatments. Proteobacteria, Bacteroidetes, and Firmicutes were the predominant phyla. Differences in phyla and class were observed among treatments and age of calf but with no consistent trends. Salmonella were detected in 9 out of 14 (64%) of the 1-wk-old calves fed nonpasteurized milk. Treponema, an important beneficial bacterium in cattle rumen, was more prevalent in the pasteurized waste milk-fed animals and became higher in the older animals from this group. Escherichia-Shigella were detected among treatments at all ages, and highest at 1 wk of age, averaging approximately 21 and 20% of all bacteria for calves fed pasteurized and nonpasteurized waste milk, respectively, and decreasing as calves aged (2.6 and 1.3%). The consistent detection of Salmonella in the younger animals fed nonpasteurized milk and its absence in all other groups is an important finding related to this feeding practice. C1 [Edrington, T. S.; Farrow, R. F.; Callaway, T. R.; Anderson, R. C.; Nisbet, D. J.] USDA ARS, Food & Feed Safety Res Unit, So Plains Agr Res Ctr, College Stn, TX 77845 USA. [Dowd, S. E.] Res & Testing Lab, Lubbock, TX 79410 USA. [Hagevoort, G. R.] New Mexico State Univ, Agr Expt Stn, Clovis, NM 88101 USA. RP Edrington, TS (reprint author), USDA ARS, Food & Feed Safety Res Unit, So Plains Agr Res Ctr, College Stn, TX 77845 USA. EM tom.edrington@ars.usda.gov NR 21 TC 9 Z9 9 U1 0 U2 25 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2012 VL 95 IS 8 BP 4519 EP 4525 DI 10.3168/jds.2011-5119 PG 7 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 976XO UT WOS:000306620900037 PM 22818466 ER PT J AU Ruesink, JL Fitzpatrick, JP Dumbauld, BR Hacker, SD Trimble, AC Wagner, EL Wisehart, LM AF Ruesink, Jennifer L. Fitzpatrick, John P. Dumbauld, Brett R. Hacker, Sally D. Trimble, Alan C. Wagner, Eric L. Wisehart, Lorena M. TI Life history and morphological shifts in an intertidal seagrass following multiple disturbances SO JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY LA English DT Article DE Allocation following disturbance; Clonal plant life-history traits; Phenotypic plasticity; Compensatory growth; Compounded perturbations ID EELGRASS ZOSTERA-MARINA; CLONAL PLANTS; USA ESTUARY; DYNAMICS; GROWTH; L.; RECOLONIZATION; MEADOW; BAY; PRODUCTIVITY AB Disturbance regimes differ in type, magnitude, and frequency, but few field experiments have considered compounded effects of disturbance. In this study, we characterized gap recovery after complete removal of eelgrass (Zostera marina) in Willapa Bay, Washington, USA. In separate experiments, we imposed two disturbance types - shoot damage and shoot removal - at different magnitudes and frequencies. We examined a broad suite of morphological and life history responses relevant to the ability of Z. marina to tolerate and recolonize following disturbance. We found that at multiple sites, 4 m(2) gaps in eelgrass meadows recovered in 2 years. Experiments revealed that cut shoots had a short-term reduction in growth, decline in shoot width in relation to the number of cutting events, and a drop in clonal branching. In contrast, in thinned plots, both clonal branching and seedling size increased. Upper-elevation intertidal plants shared many traits of cut plants, particularly lower growth and size, but maintained similar clonal branching and shoot densities as at lower intertidal elevations. Sexual reproduction appeared inflexible to disturbances imposed over the summer experimental period but differed by elevation, with upper-elevation plants showing increased flowering, lower seedling densities, but higher seedling survival. Effects of multiple disturbances were generally additive even though individually their effects varied in magnitude and direction. One exception was an antagonism that appeared in sheath width: cutting had less effect at upper than lower elevation. Overall, Z. marina tolerated shoot damage through remodeling that reduced shoot size, and shoots compensated following loss of neighbors via enhanced clonal branching. Recolonization in this estuary should be further accelerated by sexual reproduction, despite weak responses to disturbance treatments, because seedling densities in late summer (4 m(-2)) contributed 20-56% of shoot births. (C) 2012 Elsevier B.V. All rights reserved. C1 [Ruesink, Jennifer L.; Fitzpatrick, John P.; Trimble, Alan C.; Wagner, Eric L.] Univ Washington, Dept Biol, Seattle, WA 98195 USA. [Dumbauld, Brett R.] USDA ARS, Newport, OR USA. [Hacker, Sally D.; Wisehart, Lorena M.] Oregon State Univ, Dept Zool, Corvallis, OR 97331 USA. RP Ruesink, JL (reprint author), Univ Washington, Dept Biol, Box 351800, Seattle, WA 98195 USA. EM ruesink@u.washington.edu FU Andrew W. Mellon Foundation; Western Regional Aquaculture Center from United States Department of Agriculture, Cooperative State Research, Education, and Extension Service [2003-38500-13198] FX For field help, we thank L McCoy, M. Hannam, S. Yang, J. and J. Fitzpatrick, and the students of 2009 Biology 433 at the University of Washington. Washington Department of Fish and Wildlife provided space for sample processing. J. Kaldy, S. Wyllie-Echeverria, and an anonymous reviewer improved the manuscript. This study was supported by the Andrew W. Mellon Foundation and the Western Regional Aquaculture Center through Grant No. 2003-38500-13198 from the United States Department of Agriculture, Cooperative State Research, Education, and Extension Service.[SS] NR 51 TC 11 Z9 10 U1 3 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-0981 EI 1879-1697 J9 J EXP MAR BIOL ECOL JI J. Exp. Mar. Biol. Ecol. PD AUG 1 PY 2012 VL 424 BP 25 EP 31 DI 10.1016/j.jembe.2012.05.002 PG 7 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 978SS UT WOS:000306766700004 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. [Gipson, Chester] AC, USDA, APHIS, Washington, DC USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 J9 LAB ANIMAL JI Lab Anim. PD AUG PY 2012 VL 41 IS 8 BP 220 EP 220 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA 982GN UT WOS:000307031100015 PM 22821042 ER PT J AU Leites, LP Rehfeldt, GE Robinson, AP Crookston, NL Jaquish, B AF Leites, Laura P. Rehfeldt, Gerald E. Robinson, Andrew P. Crookston, Nicholas L. Jaquish, Barry TI POSSIBILITIES AND LIMITATIONS OF USING HISTORIC PROVENANCE TESTS TO INFER FOREST SPECIES GROWTH RESPONSES TO CLIMATE CHANGE SO NATURAL RESOURCE MODELING LA English DT Article DE Climate-change response functions; provenance tests; genotype by environment interaction; provenance transfer functions; Larix occidentalis Nutt; linear mixed-effects models ID PREDICT OPTIMAL-GROWTH; DOUGLAS-FIR; LARIX-OCCIDENTALIS; PINUS-CONTORTA; BLACK SPRUCE; JACK PINE; POPULATIONS; REFORESTATION; ADAPTATIONS; ENVIRONMENT AB . Under projected changes in global climate, the growth and survival of existing forests will depend on their ability to adjust physiologically in response to environmental change. Quantifying their capacity to adjust and whether the response is species- or population-specific is important to guide forest management strategies. New analyses of historic provenance tests data are yielding relevant insights about these responses. Yet, differences between the objectives used to design the experiments and current objectives impose limitations to what can be learned from them. Our objectives are (i) to discuss the possibilities and limitations of using such data to quantify growth responses to changes in climate and (ii) to present a modeling approach that creates a species- and population-specific model. We illustrate the modeling approach for Larix occidentalis Nutt. We conclude that the reanalysis of historic provenance tests data can lead to the identification of species that have population-specific growth responses to changes in climate, provide estimates of optimum transfer distance for populations and species, and provide estimates of growth changes under different climate change scenarios. Using mixed-effects modeling techniques is a sound statistical approach to overcome some of the limitations of the data. C1 [Leites, Laura P.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Rehfeldt, Gerald E.; Crookston, Nicholas L.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Robinson, Andrew P.] Univ Melbourne, ACERA, Melbourne, Vic 3010, Australia. [Robinson, Andrew P.] Univ Melbourne, Dept Math & Stat, Melbourne, Vic 3010, Australia. [Jaquish, Barry] British Columbia Minist Forests Lands & Nat Resou, Kalamalka Forestry Ctr, Vernon, BC V1B 2C7, Canada. RP Leites, LP (reprint author), Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. EM lpl3@psu.edu; jrehfeldt@gmail.com; A.Robinson@ms.unimelb.edu.au; ncrookston@fs.fed.us; barry.jaquish@gov.bc.ca OI Robinson, Andrew/0000-0002-0509-6043 NR 36 TC 13 Z9 16 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0890-8575 J9 NAT RESOUR MODEL JI Nat. Resour. Model. PD AUG PY 2012 VL 25 IS 3 BP 409 EP 433 DI 10.1111/j.1939-7445.2012.00129.x PG 25 WC Environmental Sciences; Mathematics, Interdisciplinary Applications SC Environmental Sciences & Ecology; Mathematics GA 982CK UT WOS:000307019100002 ER PT J AU Schwalm, CR Williams, CA Schaefer, K Baldocchi, D Black, TA Goldstein, AH Law, BE Oechel, WC Kyaw, TPU Scott, RL AF Schwalm, Christopher R. Williams, Christopher A. Schaefer, Kevin Baldocchi, Dennis Black, T. Andrew Goldstein, Allen H. Law, Beverly E. Oechel, Walter C. Kyaw Tha Paw U Scott, Russel L. TI Reduction in carbon uptake during turn of the century drought in western North America SO NATURE GEOSCIENCE LA English DT Article ID UNITED-STATES; SEVERITY INDEX; CLIMATE; PROJECTIONS; 21ST-CENTURY; SNOWPACK; DATASET; BALANCE; WATER; MODEL AB Fossil fuel emissions aside, temperate North America is a net sink of carbon dioxide at present(1-3). Year-to-year variations in this carbon sink are linked to variations in hydroclimate that affect net ecosystem productivity(3,4). The severity and incidence of climatic extremes, including drought, have increased as a result of climate warming(5-8). Here, we examine the effect of the turn of the century drought in western North America on carbon uptake in the region, using reanalysis data, remote sensing observations and data from global monitoring networks. We show that the area-integrated strength of the western North American carbon sink declined by 30-298 Tg C yr(-1) during the 2000-2004 drought. We further document a pronounced drying of the terrestrial biosphere during this period, together with a reduction in river discharge and a loss of cropland productivity. We compare our findings with previous palaeoclimate reconstructions(7) and show that the last drought of this magnitude occurred more than 800 years ago. Based on projected changes in precipitation and drought severity, we estimate that the present mid-latitude carbon sink of 177-623 Tg C yr(-1) in western North America could disappear by the end of the century. C1 [Schwalm, Christopher R.] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86001 USA. [Williams, Christopher A.] Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. [Schaefer, Kevin] Univ Colorado, Natl Snow & Ice Data Ctr, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Baldocchi, Dennis; Goldstein, Allen H.] Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Baldocchi, Dennis; Goldstein, Allen H.] Univ Calif Berkeley, Berkeley Atmospher Sci Ctr, Berkeley, CA 94720 USA. [Black, T. Andrew] Univ British Columbia, Fac Land & Food Syst, Vancouver, BC V6T 1Z4, Canada. [Goldstein, Allen H.] Univ Calif Berkeley, Dept Civil & Environm Engn, Berkeley, CA 94720 USA. [Law, Beverly E.] Oregon State Univ, Earth Syst Sci Div, Corvallis, OR 97330 USA. [Oechel, Walter C.] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Oechel, Walter C.] Fdn Edmund Mach, I-38010 San Michele All Adige, TN, Italy. [Kyaw Tha Paw U] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Scott, Russel L.] USDA ARS, SW Watershed Res Ctr, Tucson, AZ 85719 USA. RP Schwalm, CR (reprint author), No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86001 USA. EM christopher.schwalm@nau.edu RI Goldstein, Allen/A-6857-2011; Oechel, Walter/F-9361-2010; Baldocchi, Dennis/A-1625-2009; Law, Beverly/G-3882-2010 OI Goldstein, Allen/0000-0003-4014-4896; Oechel, Walter/0000-0002-3504-026X; Baldocchi, Dennis/0000-0003-3496-4919; Law, Beverly/0000-0002-1605-1203 FU US National Science Foundation [ATM-0910766, F1137306/MIT, 5710003122]; NASA Terrestrial Ecology award [NNX10AR68G (2N041)]; AmeriFlux (the Office of Science (BER), US Department of Energy [DE-FG02-04ER63917, DE-FG02-04ER63911] FX C.R.S., C. A. W. and K. S. were supported by the US National Science Foundation grant ATM-0910766. C. A. W. was additionally supported through NASA Terrestrial Ecology award NNX10AR68G (2N041). B. E. L. was supported by AmeriFlux (the Office of Science (BER), US Department of Energy (DE-FG02-04ER63917 and DE-FG02-04ER63911)). K. T. P. U. was supported by the US National Science Foundation grant F1137306/MIT subaward 5710003122 to the University of California, Davis. We acknowledge the World Climate Research Programme's Working Group on Coupled Modelling, which is responsible for CMIP, and we thank the climate modelling groups (Supplementary Table S4) for producing and making available their model output. For CMIP the US Department of Energy's Program for Climate Model Diagnosis and Intercomparison provided coordinating support and led development of software infrastructure in partnership with the Global Organization for Earth System Science Portals. CarbonTracker 2011 results provided by NOAA ESRL, Boulder, Colorado, USA from the website at http://carbontracker.noaa.gov. Jena CO2 inversion results provided courtesy of C. Rodenbeck, Max Planck Institute for Biogeochemistry, Jena, Germany. NR 30 TC 83 Z9 85 U1 9 U2 83 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1752-0894 EI 1752-0908 J9 NAT GEOSCI JI Nat. Geosci. PD AUG PY 2012 VL 5 IS 8 BP 551 EP 556 DI 10.1038/NGEO1529 PG 6 WC Geosciences, Multidisciplinary SC Geology GA 983DR UT WOS:000307099000012 ER PT J AU Oh, J Bowling, JJ Carroll, JF Demirci, B Baser, KHC Leininger, TD Bernier, UR Hamann, MT AF Oh, Joonseok Bowling, John J. Carroll, John F. Demirci, Betul Baser, K. Huesnue Can Leininger, Theodor D. Bernier, Ulrich R. Hamann, Mark T. TI Natural product studies of U.S. endangered plants: Volatile components of Lindera melissifolia (Lauraceae) repel mosquitoes and ticks SO PHYTOCHEMISTRY LA English DT Article DE Lindera melissifolia; Lauraceae; Pondberry; Endangered species; Arthropod repellents; beta-Caryophyllene; alpha-Humulene; Germacrene D; beta-Elemene ID AMBLYOMMA-AMERICANUM ACARI; IXODES-SCAPULARIS; ESSENTIAL OILS; AEDES-AEGYPTI; IXODIDAE; DEET; PROTECTION; FIELD; LEAF; SKIN AB The number of endangered plant species in the U.S. is significant, yet studies aimed towards utilizing these plants are limited. Ticks and mosquitoes are vectors of significant pathogenic diseases of humans. Repellents are critical means of personal protection against biting arthropods and disease transmission. The essential oil and solvent extracts from Lindera melissifolia (Walt.) Blume (Lauraceae) (pondberry) drupes were gathered and analyzed by GC and GC-MS. The essential oil obtained from this endangered plant showed a significant dose dependent repellency of ticks and a moderate mosquito repellent effect while the subsequent hexanes extract was completely ineffective. Fractional freezing enriched the tick repellent components of the essential oil. Several known tick repellent components were recognized by the GC-MS comparison of the resulting fractions and beta-caryophyllene, alpha-humulene, germacrene D and beta-elemene warrant evaluations for tick repellency. Identifying pondberry as a potential renewable source for a broad spectrum repellent supports efforts to conserve similar U.S. endangered or threatened plant species. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Oh, Joonseok; Bowling, John J.; Hamann, Mark T.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. [Carroll, John F.] USDA, ARS, IIBBL, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Demirci, Betul] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. [Baser, K. Huesnue Can] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. [Leininger, Theodor D.] USDA, Forest Serv, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. [Bernier, Ulrich R.] USDA, ARS, CMAVE, Gainesville, FL 32608 USA. RP Hamann, MT (reprint author), Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. EM mthamann@olemiss.edu RI Baser, K. Husnu Can/C-3109-2008; OI Baser, Kemal Husnu Can/0000-0003-2710-0231 FU NIH National Center for Research Resources [C06 RR-14503-01]; Kraft Food Global Inc. FX The authors thank Dr. Desmond Slade, Dr. Mei Wang and Dr. Nurhayat Tabanca for their technical assistance and Dr. Daneel Ferreira for manuscript prereview. We are grateful to Dr. Matt Kramer, USDA, ARS, Biometrical Consulting Service, Beltsville Agricultural Research Center, Beltsville, MD, for analyzing the tick data. We also thank James McCrary, Gabrielle Woodford, and Abdul Saboor Khan, USDA, ARS, IIBBL, Beltsville, MD, for conducting behavioral bioassays with ticks. This investigation was conducted in a facility constructed with support from research facilities improvement program C06 RR-14503-01 from the NIH National Center for Research Resources. Financial support was obtained from Kraft Food Global Inc. NR 54 TC 12 Z9 12 U1 0 U2 24 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9422 J9 PHYTOCHEMISTRY JI Phytochemistry PD AUG PY 2012 VL 80 BP 28 EP 36 DI 10.1016/j.phytochem.2012.05.001 PG 9 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 978GW UT WOS:000306729800003 PM 22704653 ER PT J AU Olson, DM Ruberson, JR Andow, DA AF Olson, D. M. Ruberson, J. R. Andow, D. A. TI Effects on stink bugs of field edges adjacent to woodland SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Chinavia hilaris; Euschistus servus; Nezara viridula; Margin effect; Perennial habitats; Forest ID EUSCHISTUS-SERVUS; HABITAT EDGES; HETEROPTERA-PENTATOMIDAE; PECAN ORCHARDS; SOUTH-CAROLINA; HEMIPTERA; COTTON; PATTERNS; POPULATIONS; FARMSCAPES AB Stink bug responses to crop edges were examined in 30 fields each of corn, peanut, cotton and soybean adjacent to woodland. A total of 20 sampling points of the crop row in 2009 and 15 sampling points in 2010, each 15 m long, was sampled along two 101-m transects running perpendicular to the woodland edge in each crop field. A random distribution of stink bugs (focusing on Euschistus servus, Nezara viridula, and Chinavia hilaris) was found most often with respect to the woodland edge of all crops examined. The exception occurred mainly in corn fields, where more stink bugs were found at the crop edge when flowering alternative hosts were present in the woodland edges. In 113 fields of four major crops grown in this area (7 of the 120 fields were excluded from analysis due to lack of stink bugs or limited woodland edges), the woodland edge was not a major source from which stink bugs colonized these crops. These results suggest that edge-specific control measures should not be concentrated at those field edges adjacent to woodlands. Published by Elsevier B.V. C1 [Olson, D. M.] USDA ARS, CPRMU, Tifton, GA 31793 USA. [Ruberson, J. R.] Univ Georgia, Dept Entomol, Tifton, GA USA. [Andow, D. A.] Univ Minnesota, Dept Entomol, St Paul, MN USA. RP Olson, DM (reprint author), USDA ARS, CPRMU, 2747 Davis Rd, Tifton, GA 31793 USA. EM dawn.olson@ars.usda.gov OI Ruberson, John/0000-0002-4475-8177 FU National Research Initiative of the USDA National Institute of Food and Agriculture [2008-02409]; Bellagio Center FX Thanks to Andy Hornbuckle and Melisa Thompson for their help in the field and Rebecca Wallace for help in identifying non-crop plant species. The project was supported by the National Research Initiative of the USDA National Institute of Food and Agriculture, grant number 2008-02409. DAA thanks the Bellagio Center for their support. NR 29 TC 9 Z9 9 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD AUG 1 PY 2012 VL 156 BP 94 EP 98 DI 10.1016/j.agee.2012.05.005 PG 5 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA 978DF UT WOS:000306720100010 ER PT J AU Aronstein, K Douglas, A AF Aronstein, Katherine Douglas, Angela TI Lessons Learned by the Managed Pollinator CAP: Impacts of Varroa Parasitism on Honey Bee Health SO AMERICAN BEE JOURNAL LA English DT Article ID GENE-EXPRESSION; DESTRUCTOR C1 [Aronstein, Katherine] USDA ARS, Honey Bee Res Unit, Weslaco, TX 78596 USA. [Douglas, Angela] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. RP Aronstein, K (reprint author), USDA ARS, Honey Bee Res Unit, Weslaco, TX 78596 USA. EM Kate.Aronstein@ars.usda.gov NR 4 TC 1 Z9 1 U1 2 U2 6 PU DADANT & SONS INC PI HAMILTON PA AMER BEE JOURNAL, HAMILTON, IL 62341 USA SN 0002-7626 J9 AM BEE J JI Am. Bee J. PD AUG PY 2012 VL 152 IS 8 BP 789 EP 790 PG 2 WC Entomology SC Entomology GA 978FB UT WOS:000306724900021 ER PT J AU Liu, YL Thibodeaux, D Gamble, G Bauer, P VanDerveer, D AF Liu, Yongliang Thibodeaux, Devron Gamble, Gary Bauer, Philip VanDerveer, Don TI Comparative Investigation of Fourier Transform Infrared (FT-IR) Spectroscopy and X-ray Diffraction (XRD) in the Determination of Cotton Fiber Crystallinity SO APPLIED SPECTROSCOPY LA English DT Article DE Fourier transform infrared spectroscopy; FT-IR spectroscopy; X-ray diffraction; XRD; Cellulose; Cotton fiber; Crystallinity index ID STRENGTH AB Despite considerable efforts in developing curve-fitting protocols to evaluate the crystallinity index (Cl) from X-ray diffraction (XRD) measurements, in its present state XRD can only provide a qualitative or semi-quantitative assessment of the amounts of crystalline or amorphous fraction in a sample. The greatest barrier to establishing quantitative XRD is the lack of appropriate cellulose standards, which are needed to calibrate the XRD measurements. In practice, samples with known CI are very difficult to prepare or determine. In a previous study,(14) we reported the development of a simple algorithm for determining fiber crystallinity information from Fourier transform infrared (FT-IR) spectroscopy. Hence, in this study we not only compared the fiber crystallinity information between FT-IR and XRD measurements, by developing a simple XRD algorithm in place of a time-consuming and subjective curve-fitting process, but we also suggested a direct way of determining cotton cellulose CI by calibrating XRD with the use of CIIR as references. C1 [Liu, Yongliang; Thibodeaux, Devron; Gamble, Gary] ARS, USDA, Cotton Qual Res Stn, Clemson, SC 29633 USA. [Bauer, Philip] ARS, USDA, Coastal Plain Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [VanDerveer, Don] Clemson Univ, Dept Chem, Clemson, SC 29634 USA. RP Liu, YL (reprint author), ARS, USDA, Cotton Struct & Qual Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM yongliang.liu@ars.usda.gov NR 18 TC 14 Z9 14 U1 3 U2 61 PU SOC APPLIED SPECTROSCOPY PI FREDERICK PA 5320 SPECTRUM DRIVE SUITE C, FREDERICK, MD 21703 USA SN 0003-7028 J9 APPL SPECTROSC JI Appl. Spectrosc. PD AUG PY 2012 VL 66 IS 8 BP 983 EP 986 DI 10.1366/12-06611 PG 4 WC Instruments & Instrumentation; Spectroscopy SC Instruments & Instrumentation; Spectroscopy GA 978DK UT WOS:000306720600017 PM 22800914 ER PT J AU Elsasser, TH Kahl, S Capuco, AV Schmidt, W AF Elsasser, T. H. Kahl, S. Capuco, A. V. Schmidt, W. TI Effects of stress on endocrine and metabolic processes and redirection: cross talk between subcellular compartments SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Proinflammatory stress; Nitric oxide; Superoxide anion; Protein nitration; Mitochondria; Membrane ID PROTEIN-TYROSINE NITRATION; NITRIC-OXIDE; XANTHINE-OXIDASE; GROWTH-HORMONE; ENDOPLASMIC-RETICULUM; ENDOTOXIN CHALLENGE; JANUS KINASE-2; MECHANISMS; PHOSPHORYLATION; CHAPERONES AB Recent advances in genome analysis and biochemical pathway mapping have advanced our understanding of how biological systems have evolved over time. Protein and DNA marker comparisons suggest that several of these systems are both ancient in origin but highly conserved into today's evolved species. However, remnants of some of the more ancient functions of these chemical systems can run in conflict with the functions that those same pathways serve in complex organisms and tissue systems today. Relevant to the present topic, nitric oxide (NO) and superoxide anion (O-2(center dot-)), ancient cellular molecules in evolutionary terms, are recognized today as both necessary for the well-being and stable health of cells but also injurious to cells as elaborated in conjunction with the cellular stress response. Why the dichotomy? This question underlies one of the basic issues challenging researchers as well as practitioners in their approach to disease management. The fundamental proinflammatory response of the innate immune system of the host is needed for pathogen control but can be injurious to tissues from "collateral damage" from NO- and O-2(center dot-) derived reactive molecules capable of affecting protein function via post-translational chemical modification. This review highlights newer aspects of the biochemistry of the NO- and O-2(center dot-)-mediated innate proinflammatory response and further show how protein and tissue damage via overproduction of reactive nitrogen and oxygen intermediary molecules such as peroxynitrite (ONOO-) might be targeted to specific epitopes of proteins. Changes in the regulation of metabolism in response to proinflammatory disease states are discussed for OH signal transduction and tissue specificity. Published by Elsevier Inc. C1 [Elsasser, T. H.] ARS, USDA, Bovine Funct Genom Lab, Beltsville Agr Res Ctr E, Beltsville, MD 20705 USA. RP Elsasser, TH (reprint author), ARS, USDA, Bovine Funct Genom Lab, Beltsville Agr Res Ctr E, Bldg 200,Rm 211, Beltsville, MD 20705 USA. EM theodore.elsasser@ars.usda.gov NR 48 TC 2 Z9 2 U1 1 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD AUG PY 2012 VL 43 IS 2 BP 132 EP 145 DI 10.1016/j.domaniend.2012.04.006 PG 14 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA 976XW UT WOS:000306621700006 PM 22608769 ER PT J AU Carroll, JA Burdick, NC Chase, CC Coleman, SW Spiers, DE AF Carroll, J. A. Burdick, N. C. Chase, C. C., Jr. Coleman, S. W. Spiers, D. E. TI Influence of environmental temperature on the physiological, endocrine, and immune responses in livestock exposed to a provocative immune challenge SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Cattle; Cold stress; Heat stress; Immunity; Swine ID THERMOREGULATORY RESPONSES; ENDOTOXIN CHALLENGE; NEONATAL PIGS; HEAT-STRESS; CATTLE; ROMOSINUANO; PIGLETS; ANGUS; COLD; SURVIVAL AB Although livestock experience many stressors throughout their life, one of the most commonly experienced, and most difficult to control, is stress caused by fluctuations in environmental temperatures that extend beyond the thermoneutral (TN) zone for an animal. In swine, cold stress has long been recognized as a main cause of neonatal morbidity and mortality. A possible explanation for this increased morbidity and mortality may be related to their inability to generate a febrile response. Previously, we reported that the acute phase immune response, including the generation of fever, after exposure to lipopolysaccharide (LPS; Escherichia coli 0111: B4; Sigma-Aldrich, St Louis, MO, USA) is substantially altered in neonatal pigs maintained in a cold environment (ie, 18 degrees C). Neonatal pigs that were maintained in a cold environment and administered LPS experienced a period of hypothermia coupled with altered endocrine and proinflammatory cytokine responses that could prove detrimental. In cattle, we previously reported differences in the acute phase immune response of two diverse breeds of Bos taurus cattle (Angus and Romosinuano) when maintained under TN conditions and exposed to LPS. More recently we have reported that differences in the stress and immune responses of Angus and Romosinuano heifers varies, depending on whether the cattle were housed at either TN or heat stress air temperatures. Our data clearly show that even intermittent periods of heat stress similar to that experienced in production environments can have significant effects on the stress and innate immune responses of cattle. Understanding the effect of thermal stress on livestock is critical to developing and implementing alternative management practices to improve their overall health and well-being. (C) 2012 Elsevier Inc. All rights reserved. C1 [Carroll, J. A.; Burdick, N. C.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Chase, C. C., Jr.; Coleman, S. W.] ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL USA. [Spiers, D. E.] Univ Missouri, Div Anim Sci, Columbia, MO USA. RP Carroll, JA (reprint author), ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov NR 26 TC 12 Z9 14 U1 4 U2 37 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD AUG PY 2012 VL 43 IS 2 BP 146 EP 153 DI 10.1016/j.domaniend.2011.12.008 PG 8 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA 976XW UT WOS:000306621700007 PM 22425434 ER PT J AU Orantes, LC Zhang, W Mian, MAR Michel, AP AF Orantes, L. C. Zhang, W. Mian, M. A. R. Michel, A. P. TI Maintaining genetic diversity and population panmixia through dispersal and not gene flow in a holocyclic heteroecious aphid species SO HEREDITY LA English DT Article DE aphid population genetics; dispersal; Aphis glycines; SNPs ID SPATIAL AUTOCORRELATION ANALYSIS; CULTIVATED HOST PLANTS; SOYBEAN APHID; MICROSATELLITE LOCI; NORTH-AMERICA; SEXUAL REPRODUCTION; HEMIPTERA APHIDIDAE; GOSSYPII GLOVER; GLYCINES; DISTANCE AB Heteroecious holocyclic aphids exhibit both sexual and asexual reproduction and alternate among primary and secondary hosts. Most of these aphids can feed on several related hosts, and invasions to new habitats may limit the number of suitable hosts. For example, the aphid specialist Aphis glycines survives only on the primary host buckthorn (Rhamnus spp.) and the secondary host soybean (Glycine max) in North America where it is invasive. Owing to this specialization and sparse primary host distribution, host colonization events could be localized and involve founder effects, impacting genetic diversity, population structure and adaptation. We characterized changes in the genetic diversity and structure across time among A. glycines populations. Populations were sampled from secondary hosts twice in the same geographical location: once after secondary colonization (early season), and again immediately before primary host colonization (late season). We tested for evidence of founder effects and genetic isolation in early season populations, and whether or not late-season dispersal restored genetic diversity and reduced fragmentation. A total of 24 single-nucleotide polymorphisms and 6 microsatellites were used for population genetic statistics. We found significantly lower levels of genotypic diversity and more genetic isolation among early season collections, indicating secondary host colonization occurred locally and involved founder effects. Pairwise F-ST decreased from 0.046 to 0.017 in early and late collections, respectively, and while genetic relatedness significantly decreased with geographical distance in early season collections, no spatial structure was observed in late-season collections. Thus, late-season dispersal counteracts the secondary host colonization through homogenization and increases genetic diversity before primary host colonization. Heredity (2012) 109, 127-134; doi:10.1038/hdy.2012.21; published online 2 May 2012 C1 [Orantes, L. C.; Zhang, W.; Mian, M. A. R.; Michel, A. P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. R.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Mian, M. A. R.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Wooster, OH 44691 USA. RP Michel, AP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM michel.70@osu.edu FU Ohio Soybean Council [OSC 10-2-03, OSC 08-2-08]; USDA-ARS; Department of Entomology, an OARDC-SEEDS Graduate Research Award; Ohio Agricultural Research and Development Center, the Ohio State University FX We thank the members of the Michel Laboratory (Geoff Parker, Jake Wenger) for assistance with data collection. SNP genotyping was performed at the MCIC with assistance from Jody Whittier. D Francis (OARDC) and three reviewers provided comments to improve the manuscript. Soybean samples were provided by K Tilmon, B Potter, D Ragsdale, E Cullen, C DiFonzo, C Krupke and T Baute. Funding was provided by the Ohio Soybean Council #OSC 10-2-03 and #OSC 08-2-08, USDA-ARS, Department of Entomology, an OARDC-SEEDS Graduate Research Award (LCO) and the Ohio Agricultural Research and Development Center, the Ohio State University. NR 59 TC 12 Z9 12 U1 1 U2 22 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X J9 HEREDITY JI Heredity PD AUG PY 2012 VL 109 IS 2 BP 127 EP 134 DI 10.1038/hdy.2012.21 PG 8 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 976FX UT WOS:000306567400007 PM 22549514 ER PT J AU Pryjma, M Apel, D Huynh, S Parker, CT Gaynor, EC AF Pryjma, Mark Apel, Dmitry Huynh, Steven Parker, Craig T. Gaynor, Erin C. TI FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection SO JOURNAL OF BACTERIOLOGY LA English DT Article ID INTRACELLULAR SURVIVAL; EPITHELIAL-CELLS; TRANSCRIPTIONAL ADAPTATION; MYCOBACTERIUM-TUBERCULOSIS; CHICKEN COLONIZATION; ENERGY-CONSERVATION; 2-COMPONENT SYSTEM; BIOFILM FORMATION; IRON ACQUISITION; OXIDATIVE STRESS AB Campylobacter jejuni is a food-borne bacterial pathogen that colonizes the intestinal tract and causes severe gastroenteritis. Interaction with host epithelial cells is thought to enhance severity of disease, and the ability of C. jejuni to modulate its metabolism in different in vivo and environmental niches contributes to its success as a pathogen. A C. jejuni operon comprising two genes that we designated fdhT (CJJ81176_1492) and fdhU (CJJ81176_1493) is conserved in many bacterial species. Deletion of fdhT or fdhU in C. jejuni resulted in apparent defects in adherence and/or invasion of Caco-2 epithelial cells when assessed by CFU enumeration on standard Mueller-Hinton agar. However, fluorescence microscopy indicated that each mutant invaded cells at wild-type levels, instead suggesting roles for FdhTU in either intracellular survival or postinvasion recovery. The loss of fdhU caused reduced mRNA levels of formate dehydrogenase (FDH) genes and a severe defect in FDH activity. Cell infection phenotypes of a mutant deleted for the FdhA subunit of FDH and an Delta fdhU Delta fdhA double mutant were similar to those of a Delta fdhU mutant, which likewise suggested that FdhU and FdhA function in the same pathway. Cell infection assays followed by CFU enumeration on plates supplemented with sodium sulfite abolished the Delta fdhU and Delta fdhA mutant defects and resulted in significantly enhanced recovery of all strains, including wild type, at the invasion and intracellular survival time points. Collectively, our data indicate that FdhTU and FDH are required for optimal recovery following cell infection and suggest that C. jejuni alters its metabolic potential in the intracellular environment. C1 [Pryjma, Mark; Apel, Dmitry; Gaynor, Erin C.] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada. [Huynh, Steven; Parker, Craig T.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. RP Gaynor, EC (reprint author), Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada. EM egaynor@mail.ubc.ca FU Canada Research Chair; Burroughs Wellcome Fund Career Development Award in the Biomedical Sciences; Canadian Institute of Health Research (CIHR); USDA Agricultural Research Service [5325-42000-047]; Burroughs Wellcome Career Development Award; CIHR [MOP-68981] FX E.C.G. is supported by a Canada Research Chair award and a Burroughs Wellcome Fund Career Development Award in the Biomedical Sciences. D.A. is supported by a Frederick Banting and Charles Best Graduate Scholarship from the Canadian Institute of Health Research (CIHR). C.T.P. is funded by USDA Agricultural Research Service CRIS project 5325-42000-047. This study and M.P. were funded by a Burroughs Wellcome Career Development Award and CIHR operating grant MOP-68981 to E.C.G. NR 84 TC 12 Z9 12 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD AUG PY 2012 VL 194 IS 15 BP 3803 EP 3813 DI 10.1128/JB.06665-11 PG 11 WC Microbiology SC Microbiology GA 977CP UT WOS:000306634300005 PM 22636777 ER PT J AU Martinez, JA Koyama, T Acra, S Mascarenhas, MR Shulman, RJ AF Martinez, J. Andres Koyama, Tatsuki Acra, Sari Mascarenhas, Maria R. Shulman, Robert J. TI Nutrition Education for Pediatric Gastroenterology, Hepatology, and Nutrition Fellows: Survey of NASPGHAN Fellowship Training Programs SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Article DE fellowship training; nutrition education; pediatric gastroenterology fellowship AB Objectives: The aim of the study was to assess the methodology and content of nutrition education during gastroenterology fellowship training and the variability among the different programs. Methods: A survey questionnaire was completed by 43 fellowship training directors of 62 active programs affiliated to the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition, including sites in the United States, Canada, and Mexico. The data were examined for patterns in teaching methodology and coverage of specific nutrition topics based on level 1 training in nutrition, which is the minimum requirement according to the published North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition fellowship training guidelines. Results: The majority of the teaching was conducted by MD-degree faculty (61%), and most of the education was provided through clinical care experiences. Only 31% of the level 1 nutrition topics were consistently covered by >80% of programs, and coverage did not correlate with the size of the programs. Competency in nutrition training was primarily assessed through questions to individuals or groups of fellows (77% and 65%, respectively). Program directors cited a lack of faculty interested in nutrition and a high workload as common obstacles for teaching. Conclusions: The methodology of nutrition education during gastroenterology fellowship training is, for the most part, unstructured and inconsistent among the different programs. The minimum level 1 requirements are not consistently covered. The development of core curriculums and learning modules may be beneficial in improving nutrition education. C1 [Martinez, J. Andres; Acra, Sari] Vanderbilt Univ, Sch Med, Dept Pediat, Div Pediat Gastroenterol Hepatol & Nutr, Nashville, TN 37212 USA. [Koyama, Tatsuki] Vanderbilt Univ, Sch Med, Dept Biostat, Nashville, TN 37212 USA. [Mascarenhas, Maria R.] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Shulman, Robert J.] Texas Childrens Hosp, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat,USDA,ARS, Houston, TX 77030 USA. RP Martinez, JA (reprint author), 2200 Childrens Way, Nashville, TN 37232 USA. EM andres.martinez@vanderbilt.edu FU NIH [DK058404]; Daffy's Foundation; USDA/ARS [6250-51000-043, P30 DK56338] FX Biostatistics services were provided by Vanderbilt University Medical Center's Digestive Disease Research Center supported by NIH grant DK058404. The present study was supported by the Daffy's Foundation, the USDA/ARS under Cooperative Agreement No. 6250-51000-043, and P30 DK56338, which funds the Texas Medical Center Digestive Disease Center. NR 4 TC 4 Z9 4 U1 0 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0277-2116 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD AUG PY 2012 VL 55 IS 2 BP 131 EP 135 DI 10.1097/MPG.0b013e31824ee535 PG 5 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA 977ZM UT WOS:000306705400009 PM 22343911 ER PT J AU Tatineni, S Dawson, WO AF Tatineni, Satyanarayana Dawson, William O. TI Enhancement or Attenuation of Disease by Deletion of Genes from Citrus Tristeza Virus SO JOURNAL OF VIROLOGY LA English DT Article ID COAT PROTEIN; RNA GENOME; MOVEMENT; INFECTION; TREES; REPLICATION; SEQUENCES; VIRION AB Stem pitting is a common virus-induced disease of perennial woody plants induced by a range of different viruses. The phenotype results from sporadic areas of the stem in which normal xylem and phloem development is prevented during growth of stems. These alterations interfere with carbohydrate transport, resulting in reduced plant growth and yield. Citrus tristeza virus (CTV), a phloem-limited closterovirus, induces economically important stem-pitting diseases of citrus. CTV has three nonconserved genes (p33, p18, and p13) that are not related to genes of other viruses and that are not required for systemic infection of some species of citrus, which allowed us to examine the effect of deletions of these genes on symptom phenotypes. In the most susceptible experimental host, Citrus macrophylla, the full-length virus causes only very mild stem-pitting symptoms. Surprisingly, we found that certain deletion combinations (p33 and p18 and/or p13) induced greatly increased stem-pitting symptoms, while other combinations (p13 or p13 plus p18) resulted in reduced stem pitting. These results suggest that the stem-pitting phenotype, which is one of more economically important disease phenotypes, can result not from a specific sequence or protein but from a balance between the expression of different viral genes. Unexpectedly, using green fluorescent protein-tagged full-length virus and deletion mutants (CTV9 Delta p33 and CTV9 Delta p33 Delta p18 Delta p13), the virus was found at pitted areas in abnormal locations outside the normal ring of phloem. Thus, increased stem pitting was associated not only with a prevention of xylem production but also with a proliferation of cells that supported viral replication, suggesting that at random areas of stems the virus can elicit changes in cellular differentiation and development. C1 [Tatineni, Satyanarayana; Dawson, William O.] Univ Florida, Inst Food & Agr Sci, Citrus Res & Educ Ctr, Lake Alfred, FL USA. [Tatineni, Satyanarayana] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Tatineni, Satyanarayana] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Dawson, WO (reprint author), Univ Florida, Inst Food & Agr Sci, Citrus Res & Educ Ctr, Lake Alfred, FL USA. EM wodtmv@ufl.edu FU Florida Citrus Production Research Advisory Board; National Research Initiative for the USDA Cooperative State Research, Education and Extension Service FX This research was supported in part by an endowment from the J. R. and Addie S. Graves family and grants from the Florida Citrus Production Research Advisory Board and the National Research Initiative for the USDA Cooperative State Research, Education and Extension Service. NR 28 TC 9 Z9 13 U1 0 U2 12 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 2012 VL 86 IS 15 BP 7850 EP 7857 DI 10.1128/JVI.00916-12 PG 8 WC Virology SC Virology GA 976VE UT WOS:000306614400012 PM 22593155 ER PT J AU Corella, D Carrasco, P Sorli, JV Coltell, O Ortega-Azorin, C Guillen, M Gonzalez, JI Saiz, C Estruch, R Ordovas, JM AF Corella, D. Carrasco, P. Sorli, J. V. Coltell, O. Ortega-Azorin, C. Guillen, M. Gonzalez, J. I. Saiz, C. Estruch, R. Ordovas, J. M. TI Education modulates the association of the FTO rs9939609 polymorphism with body mass index and obesity risk in the Mediterranean population SO NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES LA English DT Article DE Obesity; FTO; Educational level; Physical activity; Mediterranean ID HEART-DISEASE RISK; LIFE-STYLE FACTORS; PHYSICAL-ACTIVITY; CARDIOVASCULAR RISK; VARIANT RS9939609; ADULT OBESITY; GENE VARIANTS; FAT MASS; OVERWEIGHT; ADOLESCENTS AB Objective: To define whether the rs9939609 FTO (fat mass and obesity associated) single nucleotide polymorphism (SNP) is associated with anthropometric measurements and its modulation by educational level in a Mediterranean population. Methods: We studied 3 independent adult samples: a random sample (n = 1580) from the general population (GP), obese hospital patients (OHP) (n = 203) and elderly subjects (n = 1027) with high cardiovascular risk (HCR). Weight and height were directly measured. Education and physical activity (PA) were measured using questionnaires. Results: The rs9939609 presented heterogeneous associations with BMI. In the GP, the minor A-allele was significantly associated with greater BMI, following a co-dominant pattern (P = 0.009), whereas in the OHP this association was recessive (P = 0.004). Conversely, we did not find a significant association with BMI in the HCR group (P < 0.596). In the GP we found a significant interaction between the FTO SNP and education (P = 0.048). In the stratified analysis, no association of the FTO SNP with greater BMI in university subjects was detected (P = 0.786), whereas the association was observed in non-university subjects (P = 0.001). The FTO x education interaction (P = 0.020) was also observed in determining obesity risk in the GP. A-allele carriers had a greater risk of being obese only if they had no university education (OR: 1.56; 95% CI: 1.09-2.23 for TA and OR: 2.01; 95% CI: 1.27-3.26 for AA subjects). The interaction of the FTO with education remained significant even after adjustment for PA. Conclusions: The association of the FTO SNP with greater BMI and obesity risk in the GP was strongly modulated by education. (C) 2010 Elsevier B. V. All rights reserved. C1 [Corella, D.; Carrasco, P.; Sorli, J. V.; Ortega-Azorin, C.; Guillen, M.; Gonzalez, J. I.; Saiz, C.] Univ Valencia, Sch Med, Genet & Mol Epidemiol Unit, Valencia 46010, Spain. [Corella, D.; Carrasco, P.; Sorli, J. V.; Ortega-Azorin, C.; Guillen, M.; Gonzalez, J. I.; Saiz, C.; Estruch, R.; Ordovas, J. M.] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid, Spain. [Coltell, O.] Univ Jaume 1, Dept Comp Languages & Syst, Castellon de La Plana, Spain. [Estruch, R.] Hosp Clin Barcelona, Dept Internal Med, Barcelona, Spain. [Ordovas, J. M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Ordovas, J. M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. RP Corella, D (reprint author), Univ Valencia, Sch Med, Genet & Mol Epidemiol Unit, Blasco Ibanez 15, Valencia 46010, Spain. EM dolores.corella@uv.es FU Ministerio de Ciencia e Innovacion [CIBER CB06/03/0035, RD07/0067/0006, PI06-1326, PI07-0954, PI08-90002, SAF-09-12304]; Generalitat Valenciana [GVACOMP2010-181, BEST2010-211, BEST2010-032]; National Heart, Lung, and Blood Institute [HL-54776]; National Institute of Diabetes and Digestive and Kidney Diseases [DK075030]; US Department of Agriculture Research [53-K06-5-10, 58-1950-9-001] FX This work was supported by grants from the Ministerio de Ciencia e Innovacion (CIBER CB06/03/0035, RD07/0067/0006, PI06-1326, PI07-0954, PI08-90002 and SAF-09-12304), the Generalitat Valenciana (GVACOMP2010-181, BEST2010-211, BEST2010-032) and the National Heart, Lung, and Blood Institute grants HL-54776, National Institute of Diabetes and Digestive and Kidney Diseases, Grant Number DK075030 and by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research. NR 34 TC 12 Z9 12 U1 1 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0939-4753 J9 NUTR METAB CARDIOVAS JI Nutr. Metab. Carbiovasc. Dis. PD AUG PY 2012 VL 22 IS 8 BP 651 EP 658 DI 10.1016/j.numecd.2010.10.006 PG 8 WC Cardiac & Cardiovascular Systems; Endocrinology & Metabolism; Nutrition & Dietetics SC Cardiovascular System & Cardiology; Endocrinology & Metabolism; Nutrition & Dietetics GA 977HF UT WOS:000306646600008 PM 21186106 ER PT J AU Bonde, MR Nester, SE Berner, DK AF Bonde, M. R. Nester, S. E. Berner, D. K. TI Effects of Daily Temperature Highs on Development of Phakopsora pachyrhizi on Soybean SO PHYTOPATHOLOGY LA English DT Article DE temperature peak ID UNITED-STATES; UREDINIOSPORE GERMINATION; WETNESS PERIODS; RUST; INFECTION; TRANSPIRATION; DURATION; GROWTH; LIGHT; DEW AB Although considerable information exists regarding the importance of moisture in the development of soybean rust, little is known about the influence of temperature. The purpose of our study was to determine whether temperature might be a significant limiting factor in the development of soybean rust in the southeastern United States. Soybean plants infected with Phakopsora pachyrhizi were incubated in temperature-controlled growth chambers simulating day and night diurnal temperature patterns representative of the southeastern United States during the growing season. At 3-day intervals beginning 12 days after inoculation, urediniospores were collected from each plant and counted. The highest numbers of urediniospores were produced when day temperatures peaked at 21 or 25 degrees C and night temperatures dipped to 8 or 12 degrees C. When day temperatures peaked at 29, 33, or 37 degrees C for a minimum of 1 h/day, urediniospore production was reduced to 36, 19, and 0%, respectively, compared with urediniospore production at the optimum diurnal temperature conditions. Essentially, no lesions developed when the daily temperature high was 37 degrees C or above. Temperature data obtained from the National Climatic Data Center showed that temperature highs during July and August in several southeastern states were too high for significant urediniospore production on 55 to 77% of days. The inhibition of temperature highs on soybean rust development in southeastern states not only limits disease locally but also has implications pertaining to spread of soybean rust into and development of disease in the major soybeanproducing regions of the Midwestern and northern states. We concluded from our results that temperature highs common to southeastern states are a factor in the delay or absence of soybean rust in much of the United States. C1 [Bonde, M. R.; Nester, S. E.; Berner, D. K.] ARS, USDA, Ft Detrick, MD 21702 USA. RP Bonde, MR (reprint author), ARS, USDA, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM morris.bonde@ars.usda.gov NR 25 TC 6 Z9 6 U1 0 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2012 VL 102 IS 8 BP 761 EP 768 DI 10.1094/PHYTO-01-12-0011-R PG 8 WC Plant Sciences SC Plant Sciences GA 977ES UT WOS:000306639800005 PM 22779743 ER PT J AU Mideros, SX Windham, GL Williams, WP Nelson, RJ AF Mideros, Santiago X. Windham, Gary L. Williams, W. Paul Nelson, Rebecca J. TI Tissue-Specific Components of Resistance to Aspergillus Ear Rot of Maize SO PHYTOPATHOLOGY LA English DT Article DE quantitative disease resistance; Zea mays ID ASSOCIATION MAPPING POPULATION; AFLATOXIN ACCUMULATION; QUANTITATIVE RESISTANCE; CONTRIBUTING RESISTANCE; LEAF-BLIGHT; FLAVUS; CORN; IDENTIFICATION; LOCI; CONTAMINATION AB Aspergillus flavus and other Aspergillus spp. infect maize and produce aflatoxins. An important control measure is the use of resistant maize hybrids. There are several reports of maize lines that are resistant to atlatoxin accumulation but the mechanisms of resistance remain unknown. To gain a better understanding of resistance, we dissected the phenotype into 10 components: 4 pertaining to the response of silk, 4 pertaining to the response of developing kernels, and 2 pertaining to the response of mature kernels to inoculation with A. flavus. In order to challenge different tissues and to evaluate multiple components of resistance, various inoculation methods were used in experiments in vitro and under field condi- tions on a panel of diverse maize inbred lines over 3 years. As is typical for this trait, significant genotype environment interactions were found for all the components of resistance studied. There was, however, significant variation in maize germplasm for susceptibility to silk and kernel colonization by A. flaw's as measured in field assays. Resistance to silk colonization has not previously been reported. A significant correlation of resistance to aflatoxin accumulation with flowering time and kernel composition traits (fiber, ash, carbohydrate, and seed weight) was detected. In addition, correlation analyses with data available in the literature indicated that lines that flower later in the season tend to be more resistant. We were not able to demonstrate that components identified in vitro were associated with reduced aflatoxin accumulation in the field. C1 [Mideros, Santiago X.; Nelson, Rebecca J.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Windham, Gary L.; Williams, W. Paul] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Nelson, Rebecca J.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Nelson, RJ (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM rjn7@cornell.edu RI Mideros, Santiago/C-5141-2012 OI Mideros, Santiago/0000-0002-8988-2964 FU McKnight Foundation FX This work was funded, in part, by The McKnight Foundation. Planting, inoculation, and phenotype evaluations in Mississippi were conducted with the help of all the personnel of the Corn Host Plant Resistance Research Unit of the United States Department of Agriculture-Agricultural Research Service. We thank M. Warburton for hosting S. X. Mideros in Mississippi for three summers and graduate and undergraduate students at R. Nelson's Maize Disease Resistance Lab for help with in vitro inoculations in New York. NR 40 TC 3 Z9 3 U1 1 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2012 VL 102 IS 8 BP 787 EP 793 DI 10.1094/PHYTO-12-11-0355 PG 7 WC Plant Sciences SC Plant Sciences GA 977ES UT WOS:000306639800008 PM 22779745 ER PT J AU Martin, FN Abed, ZG Baldi, Y Ivors, K AF Martin, Frank N. Abed, Z. Gloria Baldi, Yilmaz Ivors, Kelly TI Identification and Detection of Phytophthora: Reviewing Our Progress, Identifying Our Needs SO PLANT DISEASE LA English DT Article ID POLYMERASE-CHAIN-REACTION; SUDDEN OAK DEATH; REAL-TIME PCR; FRAGMENT-LENGTH-POLYMORPHISM; INTERNAL TRANSCRIBED SPACER; PARASITICA VAR. NICOTIANAE; INFECTED-PLANT TISSUES; CYTOCHROME-OXIDASE-II; REDWOOD-TANOAK FOREST; MOLECULAR TOOL BOX C1 [Martin, Frank N.] ARS, USDA, Crop Improvement & Protect Res Unit, Salinas, CA USA. [Abed, Z. Gloria] USDA, APHIS, PPQ, CPHST,Beltsville Lab, Bethesda, MD USA. [Baldi, Yilmaz] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Ivors, Kelly] NC State Univ, Dept Plant Pathol, Mt Hort Crops Res & Extens Ctr, Mills River, NC USA. RP Martin, FN (reprint author), ARS, USDA, Crop Improvement & Protect Res Unit, Salinas, CA USA. EM Frank.Martin@ars.usda.gov RI Balci, Yilmaz/B-3420-2013 NR 300 TC 63 Z9 66 U1 2 U2 67 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1080 EP 1103 DI 10.1094/PDIS-12-11-1036-FE PG 24 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400001 ER PT J AU Samac, DA Foster-Hartnett, D AF Samac, Deborah A. Foster-Hartnett, Dawn TI Effect of Glyphosate Application on Foliar Diseases in Glyphosate-Tolerant Alfalfa SO PLANT DISEASE LA English DT Article ID MEDICAGO-SATIVA PATHOSYSTEM; SHIKIMATE PATHWAY; RESISTANT; PATHOGENS; RUST; HERBICIDES; INFECTION; WHEAT; IOWA AB Samac, D. A., and Foster-Hartnett. D. 2012. Effect of glyphosate application on foliar diseases in glyphosate-tolerant alfalfa. Plant Dis. 96:1104-1110. Glyphosate, the active ingredient in Roundup herbicide, inhibits 5-enol-pyruvyl shikimate 3-phophate synthase (EPSPS), an enzyme found in plants, fungi, and bacteria. Plants engineered for glyphosate tolerance with a glyphosate-insensitive EPSPS take up and translocate the herbicide throughout the plant. In greenhouse experiments, we found that application of glyphosate at the recommended field application rate completely controlled alfalfa rust (Uromyces striatus) on 4-week-old plants inoculated with the fungus 3 days after glyphosate treatment. Control was effective in all seven cultivars tested. The level of protection declined with time after application, indicating that control is transitory and protection declined with time after inoculation, suggesting that protective treatments have fungistatic activity. Complete control of rust was obtained when glyphosate was applied up to 10 days after inoculation with rust spores, indicating that the herbicide also has curative activity. Treatment increased protection from anthracnose, caused by Colletotrichum trifolii, a hemibiotrophic pathogen, and reduced symptom severity for spring black stem and leaf spot, caused by Phoma medicaginis, a necrotrophic pathogen. These results indicate that glyphosate could be used to help manage foliar diseases in glyphosate-tolerant alfalfa. C1 [Samac, Deborah A.] Univ Minnesota, USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [Samac, Deborah A.; Foster-Hartnett, Dawn] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. RP Samac, DA (reprint author), Univ Minnesota, USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. EM debby.samac@ars.usda.gov NR 27 TC 2 Z9 2 U1 2 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1104 EP 1110 DI 10.1094/PDIS-08-11-0715-RE PG 7 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400002 ER PT J AU Cross, C Wrather, A Fothergill, K Shannon, G Li, SX Shumway, C Rupe, J AF Cross, Cory Wrather, Allen Fothergill, Kent Shannon, Grover Li, Shuxian Shumway, Calvin Rupe, John TI Effect of Lactofen, Azoxystrobin, and Genotypes on Charcoal Rot, Phomopsis Seed Decay, and Pod and Stem Blight in Soybean SO PLANT DISEASE LA English DT Article ID INFECTION; LONGICOLLA; CULTIVAR; QUALITY AB Cross, C., Wrather, A., Fothergill, K., Shannon, G., Li, S., Shumway, C., and Rupe, J. 2012. Effect of lactofen, azoxystrobin, and genotypes on charcoal rot, Phomopsis seed decay, and pod and stem blight in soybean. Plant Dis. 96:1154-1158. Yield-limiting diseases such as charcoal rot and Phomopsis seed decay have a significant impact on the economic potential for soybean because there are few methods for management of these diseases. The objectives of this study were to determine the development of charcoal rot, infection of seed by Phomopsis spp., and severity of pod and stem blight on Asgrow 4403, Delta Pine 5806, United States Department of Agriculture-introduced DT 97-4290 and plant introduction (PI) number PI 567562A, and Asgrow 4403 treated and not treated with lactofen or azoxystrobin. This is the first report of high levels of resistance in PI 567562A to charcoal rot, and resistance in this PI was greater than for DT 97-4290. Application of lactofen at growth stage R1 and azoxystrobin at either planting, R3, or R6 had no significant impact on severity of charcoal rot, percentage of harvested seed infected by Phomopsis spp., or severity of pod and stem blight on genotype Asgrow 4403. Of four genotypes evaluated, none were resistant to infection by Phomopsis spp. The genotypes Asgrow 4403, DP 5806, and DT 974290 were susceptible to pod and stem blight and PI 567562A was resistant. C1 [Li, Shuxian] USDA ARS, Stoneville, MS 38776 USA. [Shumway, Calvin] Arkansas State Univ, Jonesboro, AR 72467 USA. [Rupe, John] Univ Arkansas, Fayetteville, AR 72701 USA. EM crosscb@missouri.edu FU University of Missouri Agriculture Experiment Station; United Soybean Board [9261] FX These studies were supported, in part, by the University of Missouri Agriculture Experiment Station. We thank the United Soybean Board for the financial support from soybean check-off dollars through funding of the project (USB number 9261) entitled "Screening Germplasm and Breeding for Resistance to Phomopsis Seed Decay in Soybean", J. Elrod for her efforts in this project, and God for guidance. NR 25 TC 1 Z9 2 U1 0 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1154 EP 1158 DI 10.1094/PDIS-09-11-0810-RE PG 5 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400009 ER PT J AU Strausbaugh, CA Wenninger, EJ Eujayl, IA AF Strausbaugh, Carl A. Wenninger, Erik J. Eujayl, Imad A. TI Management of Severe Curly Top in Sugar Beet with Insecticides SO PLANT DISEASE LA English DT Article ID WESTERN UNITED-STATES; NEONICOTINOID INSECTICIDES; SYSTEMIC INSECTICIDES; NILAPARVATA-LUGENS; BROWN PLANTHOPPER; VIRUS; RESISTANCE; PLANTS; GEMINIVIRUS; IRAN AB Strausbaugh, C. A., Wenninger, E. J., and Eujayl, I. A. 2012. Management of severe curly top in sugar beet with insecticides. Plant Dis. 96:1159-1164. Curly top, caused by Curtovirus spp., is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. The insecticide seed treatment Poncho Beta has proven to be effective in controlling curly top in sugar beet but was only evaluated under light to moderate disease pressure. Thus, the insecticide seed treatments Poncho Beta, Nips It INSIDE, and Cruiser Force were evaluated under severe curly top pressure (six viruliferous beet leafhoppers per plant) in field studies during the 2010 and 2011 growing seasons on two commercial sugar beet cultivars. In addition, the foliar insecticides Movento, Provado, and Scorpion were also evaluated. The seed treatments and Scorpion reduced curly top symptoms by 33 to 41% (P < 0.0001) and increased root yield by 55 to 95% (P < 0.0001), sucrose content by 6.5 to 7.2% (P = 0.0013 to < 0.0001), and estimated recoverable sucrose by 58 to 96% (P < 0.0001) when compared with the untreated check. Movento and Provado did not improve control beyond that provided by Poncho Beta. Even under severe disease pressure 50 to 55 days after planting, neonicotinoid seed treatments can effectively reduce curly top, increase yield, and help protect against early-season insect pest pressure. C1 [Strausbaugh, Carl A.; Eujayl, Imad A.] USDA ARS NWISRL, Kimberly, ID 83341 USA. [Wenninger, Erik J.] Univ Idaho, Kimberly, ID 83341 USA. RP Strausbaugh, CA (reprint author), USDA ARS NWISRL, Kimberly, ID 83341 USA. EM carl.strausbaugh@ars.usda.gov OI Strausbaugh, Carl/0000-0001-6773-1375 FU United States Department of Agriculture CRIS [5368-21220-002-00D]; Snake River Sugarbeet Growers; Beet Sugar Development Foundation FX These data support the objectives of the United States Department of Agriculture CRIS project 5368-21220-002-00D. We thank the Snake River Sugarbeet Growers and Beet Sugar Development Foundation for supporting our research work and T. Brown, J. Reed, and D. Kenney for their technical support. NR 39 TC 4 Z9 4 U1 0 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1159 EP 1164 DI 10.1094/PDIS-01-12-0106-RE PG 6 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400010 ER PT J AU Twizeyimana, M Hill, CB Pawlowski, M Paul, C Hartman, GL AF Twizeyimana, M. Hill, C. B. Pawlowski, M. Paul, C. Hartman, G. L. TI A Cut-Stem Inoculation Technique to Evaluate Soybean for Resistance to Macrophomina phaseolina SO PLANT DISEASE LA English DT Article ID CHARCOAL ROT; HETERODERA-GLYCINES; KANSAS; KENYA; SOIL AB Twizeyimana, M., Hill, C. B., Pawlowski, M., Paul, C., and Hartman, G. L. 2012. A cut-stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina. Plant Dis. 96:1210-1215. Charcoal rot of soybean is caused by the fungal pathogen Macrophomina phaseolina. Effective and reliable techniques to evaluate soybean for resistance to this fungus are needed to work toward a management scheme that would utilize host resistance. Three experiments were conducted to investigate the use of a cut-stem inoculation technique to evaluate soybean genotypes for resistance to M. phaseolina. The first experiment compared aggressiveness of M. phaseolina isolates collected from soybean on different soybean genotypes. Significant (P < 0.05) differences among the isolates and genotypes for relative area under disease progress curve (RAUDPC) were found without a significant isolate genotype interaction. The second experiment compared 14 soybean genotypes inoculated with M. phaseolina in multiple trials conducted in two environments, the greenhouse and growth chamber. Significant (P < 0.05) differences among environments and highly significant (P < 0.001) differences among soybean genotypes for RAUDPC were found. The environment genotype interaction was nonsignificant (P > 0.05). Soybean genotypes DT97-4290, DT98-7553, DT98-17554, and DT99-16864 had significantly (P < 0.05) lower RAUDPC than 7 of the 14 genotypes. The third experiment evaluated resistance in selected Phaseolus spp. and soybean genotypes. The range of RAUDPC for Phaseolus spp. was similar to that of soybean. The Phaseolus lunatus 'Bush Baby Lima' had significantly (P < 0.05) lower RAUDPC than P. vulgaris genotypes evaluated. The cutstem inoculation technique, which has several advantages over field tests, successfully distinguished differences in aggressiveness among M. phaseolina isolates and relative differences among soybean genotypes for resistance to M. phaseolina comparable with results of field tests. C1 [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Twizeyimana, M.; Hill, C. B.; Pawlowski, M.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Twizeyimana, Mathias/0000-0003-2173-6693 FU United Soybean Board FX We thank the United Soybean Board for their support of this research; and T. Herman, J. C. Rupe, and K. Whiting for their editorial comments. NR 39 TC 7 Z9 7 U1 1 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1210 EP 1215 DI 10.1094/PDIS-02-12-0126-RE PG 6 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400018 ER PT J AU Kolmer, JA Long, DL Hughes, ME AF Kolmer, J. A. Long, D. L. Hughes, M. E. TI Physiologic Specialization of Puccinia triticina on Wheat in the United States in 2010 SO PLANT DISEASE LA English DT Article ID LEAF RUST RESISTANCE; F-SP TRITICI; NORTH-AMERICA; VIRULENCE; POPULATIONS; DIVERSITY; CANADA AB Kolmer, J. A., Long, D. L., and Hughes, M. E. 2012. Physiologic specialization of Puccinia triticina on wheat in the United States in 2010. Plant Dis. 96:1216-1221. Collections of Puccinia triticina were obtained from rust-infected leaves provided by cooperators throughout the United States and from wheat fields and breeding plots by United States Department of Agriculture Agricultural Research Service personnel and cooperators in the Great Plains, Ohio River Valley, southeastern states, Oregon, and Washington State in order to determine the virulence of the wheat leaf rust population in 2010. Single uredinial isolates (537 total) were derived from the collections and tested for virulence phenotype on 19 lines of 'Thatcher' wheat and a winter wheat line that are near-isogenic for 20 leaf rust resistance genes. In 2010, 38 virulence phenotypes were described in the United States. Virulence phenotypes MLDSD, TDBJG, and TCRKG were the three most common phenotypes. Phenotype MLDSD is virulent to Lr17 and Lr39/Lr41 and was widely distributed throughout the United States. Phenotype TDBJG is virulent to Lr24 and was found in both the soft red winter wheat and hard red winter wheat regions. Phenotype TCRKG is virulent to Lr11, Lr18, and Lr26 and was found mostly in the soft red winter wheat region in the eastern United States. Virulence to Lr21 was found for the first time in North America in isolates collected from spring wheat cultivars in North Dakota and Minnesota. C1 [Kolmer, J. A.; Long, D. L.; Hughes, M. E.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Jim.Kolmer@ars.usda.gov NR 19 TC 8 Z9 8 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1216 EP 1221 DI 10.1094/PDIS-01-12-0048-SR PG 6 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400019 ER PT J AU Schubert, TS Dewdney, MM Peres, NA Palm, ME Jeyaprakash, A Sutton, B Mondal, SN Wang, NY Rascoe, J Picton, DD AF Schubert, T. S. Dewdney, M. M. Peres, N. A. Palm, M. E. Jeyaprakash, A. Sutton, B. Mondal, S. N. Wang, N. -Y. Rascoe, J. Picton, D. D. TI First Report of Guignardia citricarpa Associated with Citrus Black Spot on Sweet Orange (Citrus sinensis) in North America SO PLANT DISEASE LA English DT News Item ID MANGIFERAE C1 [Schubert, T. S.; Jeyaprakash, A.; Sutton, B.] Div Plant Ind, Florida Dept Agr & Consumer Serv, Gainesville, FL 32614 USA. [Dewdney, M. M.; Mondal, S. N.; Wang, N. -Y.] Univ Florida, IFAS, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Peres, N. A.] Univ Florida, IFAS, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Palm, M. E.; Rascoe, J.; Picton, D. D.] APHIS, USDA, Beltsville, MD 20705 USA. RP Schubert, TS (reprint author), Div Plant Ind, Florida Dept Agr & Consumer Serv, Gainesville, FL 32614 USA. RI Dewdney, Megan/C-5948-2013 NR 3 TC 13 Z9 13 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1225 EP 1225 DI 10.1094/PDIS-01-12-0101-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400028 ER PT J AU Serrato-Diaz, LM Latoni-Brailowsky, EI Rivera-Vargas, LI Goenaga, R French-Monar, RD AF Serrato-Diaz, L. M. Latoni-Brailowsky, E. I. Rivera-Vargas, L. I. Goenaga, R. French-Monar, R. D. TI First Report of Gliocephalotrichum bulbilium and G. simplex Causing Fruit Rot of Rambutan in Puerto Rico SO PLANT DISEASE LA English DT News Item C1 [Serrato-Diaz, L. M.; French-Monar, R. D.] Texas A&M Syst, Texas AgriLife Extens Serv, Dept Plant Pathol & Microbiol, Amarillo, TX USA. [Latoni-Brailowsky, E. I.; Rivera-Vargas, L. I.] Univ Puerto Rico Mayaguez Campus, Dept Crops & Agroenvironm Sci, Mayaguez, PR USA. [Goenaga, R.] ARS, USDA, Trop Agr Res Stn, Mayaguez, PR USA. RP Serrato-Diaz, LM (reprint author), Texas A&M Syst, Texas AgriLife Extens Serv, Dept Plant Pathol & Microbiol, Amarillo, TX USA. NR 4 TC 2 Z9 2 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1225 EP 1226 DI 10.1094/PDIS-02-12-0210-PDN PG 2 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400029 ER PT J AU Ostry, ME Moore, MJ Anderson, NA AF Ostry, M. E. Moore, M. J. Anderson, N. A. TI A Second Spore Stage Confirmed for Apioplagiostoma populi, the Causal Agent of Bronze Leaf Disease of Populus in Minnesota SO PLANT DISEASE LA English DT News Item C1 [Ostry, M. E.; Moore, M. J.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. [Anderson, N. A.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. RP Ostry, ME (reprint author), US Forest Serv, USDA, No Res Stn, St Paul, MN USA. NR 3 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2012 VL 96 IS 8 BP 1227 EP 1227 DI 10.1094/PDIS-03-12-0289-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 976XJ UT WOS:000306620400034 ER PT J AU Hamerlynck, EP Scott, RL Barron-Gafford, GA Cavanaugh, ML Moran, M Huxman, TE AF Hamerlynck, Erik P. Scott, Russell L. Barron-Gafford, Greg A. Cavanaugh, Michelle L. Susan Moran, M. Huxman, Travis E. TI Cool-season whole-plant gas exchange of exotic and native semiarid bunchgrasses SO PLANT ECOLOGY LA English DT Article DE Bush muhly; Evapotranspiration; Lehmann lovegrass; Photosynthesis; Respiration; Savanna; Soil water ID NORTHERN TEMPERATE GRASSLAND; NET PRIMARY PRODUCTION; WATER-USE EFFICIENCY; SOIL CO2 FLUX; NONNATIVE GRASSES; LEHMANN LOVEGRASS; BIOMASS PRODUCTION; SOUTHERN ARIZONA; DESERT ECOSYSTEM; MOJAVE DESERT AB The success of invasive aridland plants may depend on their utilization of precipitation not fully exploited by native species, which could lead to seasonally altered ecosystem carbon and water fluxes. We measured volumetric soil water across 25-cm profiles (theta(25cm)) and springtime whole-plant water- and carbon-fluxes of the exotic Lehmann lovegrass (Eragrostis lehmanniana) and a native bunchgrass, bush muhly (Muhlenbergia porteri), following typical (55 mm in 2009) and El Nio-enhanced accumulations (154 mm in 2010) in a SE Arizona savanna. Across both years, theta(25cm) was higher under lovegrass plots, with similar evapotranspiration (ET) between lovegrass and bush muhly plots. However, in 2010 transpiration (T) was higher in bush muhly than lovegrass, implying higher soil evaporation in lovegrass plots maintained similar ET. Net ecosystem carbon dioxide exchange (NEE) was similar between lovegrass and bush muhly plots in 2009, but was more negative in bush muhly plots following El Nio, indicating greater CO2 assimilation. Ecosystem respiration (R (eco)) and gross ecosystem photosynthesis (GEP) were similar between lovegrass and bush muhly plots in 2009, but were higher in bush muhly plots in 2010. As a result, lovegrass plots reduced ecosystem water-use efficiency (WUEe = NEE/ET), while bush muhly WUEe remained constant between 2009 and 2010. Concurrent whole-plant WUE (WUEp = GEP/T) did not change in lovegrass plots, but increased in bush muhly plots between these years. We concluded that cool-season precipitation use is not a component of Lehmann lovegrass invasive success, but that the change in ET partitioning and attendant shifts in cool-season WUEe may increase interannual variation in ecosystem water- and carbon-exchange dynamics in the water-limited systems it dominates. C1 [Hamerlynck, Erik P.; Scott, Russell L.; Cavanaugh, Michelle L.; Susan Moran, M.] ARS, USDA, SW Watershed Res Ctr, Tucson, AZ USA. [Barron-Gafford, Greg A.; Huxman, Travis E.] Univ Arizona, Tucson, AZ USA. RP Hamerlynck, EP (reprint author), ARS, USDA, SW Watershed Res Ctr, Tucson, AZ USA. EM erik.hamerlynck@ars.usda.gov NR 60 TC 5 Z9 6 U1 0 U2 43 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD AUG PY 2012 VL 213 IS 8 BP 1229 EP 1239 DI 10.1007/s11258-012-0081-x PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 977VZ UT WOS:000306694200003 ER PT J AU Soy, J Leivar, P Gonzalez-Schain, N Sentandreu, M Prat, S Quail, PH Monte, E AF Soy, Judit Leivar, Pablo Gonzalez-Schain, Nahuel Sentandreu, Maria Prat, Salome Quail, Peter H. Monte, Elena TI Phytochrome-imposed oscillations in PIF3 protein abundance regulate hypocotyl growth under diurnal light/dark conditions in Arabidopsis SO PLANT JOURNAL LA English DT Article DE PIF3; hypocotyl elongation; short day; phytochrome-mediated degradation; transcriptional regulation; Arabidopsis ID BHLH TRANSCRIPTION FACTORS; SHADE-AVOIDANCE-RESPONSE; RED-LIGHT; CIRCADIAN CLOCK; INTERACTING FACTOR-3; MEDIATED DEGRADATION; HIGH-TEMPERATURE; CELL ELONGATION; THALIANA; BIOSYNTHESIS AB Arabidopsis seedlings display rhythmic growth when grown under diurnal conditions, with maximal elongation rates occurring at the end of the night under short-day photoperiods. Current evidence indicates that this behavior involves the action of the growth-promoting bHLH factors PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) and PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) at the end of the night, through a coincidence mechanism that combines their transcriptional regulation by the circadian clock with control of protein accumulation by light. To assess the possible role of PIF3 in this process, we have analyzed hypocotyl responses and marker gene expression in pif single- and higher-order mutants. The data show that PIF3 plays a prominent role as a promoter of seedling growth under diurnal light/dark conditions, in conjunction with PIF4 and PIF5. In addition, we provide evidence that PIF3 functions in this process through its intrinsic transcriptional regulatory activity, at least in part by directly targeting growth-related genes, and independently of its ability to regulate phytochrome B (phyB) levels. Furthermore, in sharp contrast to PIF4 and PIF5, our data show that the PIF3 gene is not subject to transcriptional regulation by the clock, but that PIF3 protein abundance oscillates under diurnal conditions as a result of a progressive decline in PIF3 protein degradation mediated by photoactivated phyB, and consequent accumulation of the bHLH factor during the dark period. Collectively, the data suggest that phyB-mediated, post-translational regulation allows PIF3 accumulation to peak just before dawn, at which time it accelerates hypocotyl growth, together with PIF4 and PIF5, by directly regulating the induction of growth-related genes. C1 [Soy, Judit; Leivar, Pablo; Gonzalez-Schain, Nahuel; Sentandreu, Maria; Monte, Elena] Campus Univ Autonoma Barcelona, Dept Mol Genet, Ctr Res Agr Genom CRAG, CSIC IRTA UAB UB, Barcelona 08193, Spain. [Prat, Salome] Campus Univ Autonoma Madrid, Dept Genet Mol Plantas, Ctr Nacl Biotecnol CSIC, Madrid 28049, Spain. [Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Quail, Peter H.] Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Monte, E (reprint author), Campus Univ Autonoma Barcelona, Dept Mol Genet, Ctr Res Agr Genom CRAG, CSIC IRTA UAB UB, Barcelona 08193, Spain. EM elena.monte@cragenomica.es RI Prat, Salome/L-9240-2014; Elena, Monte/I-7221-2015; Leivar, Pablo/D-7808-2017 OI Prat, Salome/0000-0003-2684-5485; Leivar, Pablo/0000-0003-4878-3684 FU CSIC [Jae-Pre_08_01049, 2010ESTCSIC-12125]; 'Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa' from the Generalitat de Catalunya; Marie Curie International Reintegration Grant [PIRG06-GA-2009-256420, 046568]; National Institutes of Health [GM-47475]; Department of Energy [DEFG03-87ER13742]; USDA Agricultural Research Service [5335-21000-027-00D]; Spanish Ministerio de Ciencia e Innovacion [BIO2006-09254, BIO2009-07675]; Generalitat de Catalunya [2009-SGR-206] FX This work was supported by a JAE pre-doctoral fellowship (Jae-Pre_08_01049) and a JAE Estancia Breve grant (2010ESTCSIC-12125 for a short stay in S.P.'s laboratory) from CSIC to J.S., a 'Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa' fellowship from the Generalitat de Catalunya (Beatriu de Pinos program) and Marie Curie International Reintegration Grant PIRG06-GA-2009-256420 to P. L., by National Institutes of Health Grant GM-47475, Department of Energy Grant DEFG03-87ER13742, and USDA Agricultural Research Service Current Research Information System Grant 5335-21000-027-00D to P. H. Q., and Marie Curie International Reintegration Grant 046568, grants from the Spanish Ministerio de Ciencia e Innovacion (BIO2006-09254 and BIO2009-07675), and a grant from the Generalitat de Catalunya (2009-SGR-206) to E.M. NR 62 TC 39 Z9 49 U1 1 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 J9 PLANT J JI Plant J. PD AUG PY 2012 VL 71 IS 3 BP 390 EP 401 DI 10.1111/j.1365-313X.2012.04992.x PG 12 WC Plant Sciences SC Plant Sciences GA 980LA UT WOS:000306893500004 PM 22409654 ER PT J AU Shim, MY Karnuah, AB Mitchell, AD Anthony, NB Pesti, GM Aggrey, SE AF Shim, M. Y. Karnuah, A. B. Mitchell, A. D. Anthony, N. B. Pesti, G. M. Aggrey, S. E. TI The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers SO POULTRY SCIENCE LA English DT Article DE tibia; broiler; tibia breaking strength; tibia mineral density; tibia ash ID LAYING HENS; SKELETAL DEVELOPMENT; COMMERCIAL BROILERS; PHOSPHORUS; CHICKENS; CALCIUM; WEAKNESS; POULTRY; FOWL; ABNORMALITIES AB Fast-growing broilers are especially susceptible to bone abnormalities, causing major problems for broiler producers. The cortical bones of fast-growing broilers are highly porous, which may lead to leg deformities. Leg problems were investigated in 6-wk-old Arkansas randombred broilers. Body weight was measured at hatch and at 6 wk. There were 8 different settings of approximately 450 eggs each. Two subpopulations, slow-growing (SG; bottom quarter, n = 511) and fast-growing (FG; top quarter, n = 545), were created from a randombred population based on their growth rate from hatch until 6 wk of age. At 6 wk of age, the broilers were processed and chilled at 4 degrees C overnight before deboning. Shank (78.27 +/- 8.06 g), drum stick (190.92 +/- 16.91 g), and thigh weights (233.88 +/- 22.66 g) of FG broilers were higher than those of SG broilers (54.39 +/- 6.86, 135.39 +/- 15.45, and 168.50 +/- 21.13 g, respectivly; P < 0.001). Tibia weights (15.36 +/- 2.28 g) of FG broilers were also greater than those of SG broilers (11.23 +/- 1.81 g; P < 0.001). Shank length (81.50 +/- 4.71 g) and tibia length (104.34 +/- 4.45 mm) of FG broilers were longer than those of SG broilers (71.88 +/- 4.66 and 95.98 +/- 4.85 mm, respectively; P < 0.001). Shank diameter (11.59 +/- 1.60 mm) and tibia diameter (8.20 +/- 0.62 mm) of FG broilers were wider than those of SG broilers (9.45 +/- 1.74, 6.82 +/- 0.58 mm, respectively; P < 0.001). Tibia breaking strength (28.42 +/- 6.37 kg) of FG broilers was higher than those of SG broiler tibia (21.81 +/- 5.89 kg; P < 0.001). Tibia density and bone mineral content (0.13 +/- 0.01 g/cm(2) and 1.29 +/- 0.23 g, respectively) of FG broilers were higher than those of SG broiler tibia (0.11 +/- 0.01 g/cm(2) and 0.79 +/- 0.1 g; P < 0.001). Tibia percentage of ash content (39.76 +/- 2.81) of FG broilers was lower than that of SG broilers (39.99 +/- 2.67; P = 0.173). Fast-growing broiler bones were longer, wider, heavier, stronger, more dense, and contained more ash than SG ones. After all parameters were calculated per unit of final BW at 6 wk, tibia density and bone ash percentage of FG broilers were lower than those of SG broilers. C1 [Shim, M. Y.; Karnuah, A. B.; Pesti, G. M.; Aggrey, S. E.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. [Mitchell, A. D.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Anthony, N. B.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. RP Aggrey, SE (reprint author), Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. EM saggrey@uga.edu NR 50 TC 16 Z9 16 U1 3 U2 42 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2012 VL 91 IS 8 BP 1790 EP 1795 DI 10.3382/ps.2011-01968 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 978DC UT WOS:000306719800007 PM 22802169 ER PT J AU Garcia, RA Phillips, JG Adeola, O AF Garcia, R. A. Phillips, J. G. Adeola, O. TI Improved prediction of meat and bone meal metabolizable energy content for ducks through in vitro methods SO POULTRY SCIENCE LA English DT Article DE duck; meat and bone meal; metabolizable energy; particle size; protein digestibility ID FISH-MEAL; DIGESTIBILITY; QUALITY; PEPSIN; BROILERS; PROTEIN AB Apparent metabolizable energy (AME) of meat and bone meal (MBM) for poultry is highly variable, but impractical to measure routinely. Previous efforts at developing an in vitro method for predicting AME have had limited success. The present study uses data from a previous publication on the AME of 12 MBM samples, determined using 288 White Pekin ducks, as well as composition data on these samples. Here, we investigate the hypothesis that 2 noncompositional attributes of MBM, particle size and protease resistance, will have utility in improving predictions of AME based on in vitro measurements. Using the same MBM samples as the previous study, 2 measurements of particle size were recorded and protease resistance was determined using a modified pepsin digestibility assay. Analysis of the results using a stepwise construction of multiple linear regression models revealed that the measurements of particle size were useful in building models for AME, but the measure of protease resistance was not. Relatively simple (4-term) and complex (7-term) models for both AME and nitrogen-corrected AME were constructed, with R-squared values ranging from 0.959 to 0.996. The rather minor analytical effort required to conduct the measurements involved is discussed. Although the generality of the results are limited by the number of samples involved and the species used, they suggest that AME for poultry can be accurately predicted through simple and inexpensive in vitro methods. C1 [Garcia, R. A.] ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Phillips, J. G.] ARS, N Atlantic Area Off, USDA, Wyndmoor, PA 19038 USA. [Adeola, O.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. RP Garcia, RA (reprint author), ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Ln, Wyndmoor, PA 19038 USA. EM Rafael.Garcia@ars.usda.gov RI Garcia, Rafael/D-2796-2009 OI Garcia, Rafael/0000-0002-5452-3929 NR 21 TC 1 Z9 1 U1 5 U2 13 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2012 VL 91 IS 8 BP 1854 EP 1859 DI 10.3382/ps.2011-01876 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 978DC UT WOS:000306719800016 PM 22802178 ER PT J AU Long, JA Conn, TL AF Long, J. A. Conn, T. L. TI Use of phosphatidylcholine to improve the function of turkey semen stored at 4 degrees C for 24 hours SO POULTRY SCIENCE LA English DT Article DE turkey; semen storage; fertility; phospholipid ID IN-VITRO STORAGE; LIPID-PEROXIDATION; SPERM MOBILITY; LIQUID STORAGE; POULTRY SEMEN; FOWL SPERMATOZOA; FERTILITY; MOTILITY; 5-DEGREES-C; VIABILITY AB The ability to store turkey semen for 24 h in vitro without a significant loss in fertility would benefit the commercial turkey industry. We investigated the use of exogenous phosphatidylcholine to improve the viability, mobility, hydrolyzing ability, and fertility of stored turkey sperm. For experiment 1, semen was diluted 1:1 with extender containing 0.0 (control), 0.5, 2.5, or 10.0 mg/mL of phosphatidylcholine labeled with a fluorochrome and maintained under aerobic conditions for 24 h at 4 degrees C. Semen aliquots were removed at 30-min intervals during the first 4 h and at 1-h intervals from 8 to 24 h of storage for fluorometric evaluation by flow cytometry. Turkey sperm incorporated labeled phosphatidylcholine in a dose-dependent manner during the first 12 h of storage (P < 0.05). At 24 h of storage, phosphatidylcholine uptake increased 7.8-fold for the 0.5-mg treatment, 9.2-fold for the 2.5-mg treatment, and 6.7-fold for the 10-mg labeled phosphatidylcholine treatment. For experiment 2, turkey semen was diluted and stored as for experiment 1 except the phosphatidylcholine was unlabeled. After 24 h, the viability, mobility, hydrolyzing ability, and fertility of turkey sperm was assessed. Supplemental phosphatidylcholine did not improve (P > 0.05) the viability or mobility of stored sperm. At 2.5 mg/mL, phosphatidylcholine improved the hydrolyzing ability of stored sperm compared with control or other phosphatidylcholine treatments (P < 0.05). The mean fertility rate of eggs from hens inseminated with control semen was 33.5% +/- 4.5. Use of 0.5, 2.5, or 10.0 mg phosphatidylcholine/mL improved (P < 0.05) the fertility rates during the first 11 wk of egg production; higher fertility rates occurred with 2.5 mg phosphatidylcholine/mL compared with other phosphatidylcholine treatments for 5 of those 11 wk (P < 0.05). We conclude that supplemental phosphatidylcholine appears to counteract the damaging effects of lipid peroxidation or enzymatic degradation during in vitro storage by providing exogenous phospholipids for incorporation into the turkey sperm plasma membrane. C1 [Long, J. A.; Conn, T. L.] USDA, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Long, JA (reprint author), USDA, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM julie.long@ars.usda.gov FU USDA-ARS Project "Development of New Technologies and Methods to Enhance the Utilization and Long-term-Storage of Poultry, Swine and Fish Germplasm" [1265-31000-093-00D] FX Supported by the USDA-ARS Project "Development of New Technologies and Methods to Enhance the Utilization and Long-term-Storage of Poultry, Swine and Fish Germplasm" (Project No. 1265-31000-093-00D). Mention of a trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by USDA or imply its approval to the exclusion of other suitable products or vendors. NR 32 TC 5 Z9 5 U1 1 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2012 VL 91 IS 8 BP 1990 EP 1996 DI 10.3382/ps.2011-02028 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 978DC UT WOS:000306719800033 PM 22802195 ER PT J AU Samuel, D Trabelsi, S AF Samuel, D. Trabelsi, S. TI Influence of color on dielectric properties of marinated poultry breast meat SO POULTRY SCIENCE LA English DT Article DE dielectric properties; meat quality; marination; poultry ID WATER-HOLDING CAPACITY; RADIO-FREQUENCY; PALE; SPECTROSCOPY; STRATEGIES; SPECTRA; FILLETS; SOFT AB The dielectric behavior of foods when exposed to radio-frequency and microwave electric fields is highly influenced by moisture content and the degree of water binding with constituents of the food materials. The ability to correlate specific food quality characteristics with the dielectric properties can lead to the development of rapid, nondestructive techniques for such quality measurements. Water-holding capacity is a critical attribute in meat quality. Up to 50% of raw poultry meat in the United States is marinated with mixtures of water, salts, and phosphates. The objective of this study was to determine if variations in breast meat color would affect the dielectric properties of marinated poultry meat over a broad frequency range from 500 MHz to 50 GHz. Poultry meat was obtained from a local commercial plant in Georgia (USA). Color and pH measurements were taken on the breast filets. Groups of breast filets were sorted into classes of pale and normal before adding marination pickup percentages of 0, 5, 10, and 15. Breast filets were vacuum-tumbled and weighed for pickup percentages. Dielectric properties of the filets were measured with a coaxial open-ended probe on samples equilibrated to 25 degrees C. Samples from pale meat exhibited higher dielectric properties than samples from normal meat. No differences could be observed between samples from pale and normal meat after marination of the samples. Overall, dielectric properties increased as the marination pickup increased (alpha = 0.05). Marination pickup strongly influenced the dielectric loss factor. Differences between samples marinated at different pickup levels were more pronounced at lower frequencies for the dielectric loss factor. As frequency increased, the differences between samples decreased. Differences in dielectric constant between samples were not as consistent as those seen with the dielectric loss factor. C1 [Samuel, D.; Trabelsi, S.] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. RP Samuel, D (reprint author), ARS, USDA, Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM darlene.samuel@ars.usda.gov NR 34 TC 3 Z9 4 U1 1 U2 22 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2012 VL 91 IS 8 BP 2011 EP 2016 DI 10.3382/ps.2011-01837 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 978DC UT WOS:000306719800036 PM 22802198 ER PT J AU Scott, AE Forsythe, KW Johnson, CL AF Scott, Aaron E. Forsythe, Kenneth W. Johnson, Cynthia L. TI National animal health surveillance: Return on investment SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE National animal health surveillance; Return on investment; Cost-benefit; Stream-based surveillance AB A weighted benefit-cost analysis (BCA) supports prioritization of animal health surveillance activities to safeguard animal agriculture industries and reduce the impact of disease on the national economy. We propose to determine the value of investment in surveillance by assessing benefits from: avoiding disease incursion and expansion modified by the probability of occurrence of the disease event, the sensitivity of systems to detect it, and the degree to which we can mitigate disease impact when detected. The weighted benefit-cost ratio is the modified value of surveillance as laid out above divided by the cost of surveillance. We propose flexible, stream-based surveillance that capitalizes on combining multiple streams of information from both specific pathogen based and non-pathogen based surveillance. This stream-based type of system provides high value with lower costs and will provide a high return for the funds invested in animal health surveillance. Published by Elsevier B.V. C1 [Scott, Aaron E.] Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Ctr Epidemiol, Ft Collins, CO 80526 USA. Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Ctr Anim Hlth, Ft Collins, CO 80526 USA. RP Scott, AE (reprint author), Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Ctr Epidemiol, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM aaron.e.scott@aphis.usda.gov NR 10 TC 3 Z9 3 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 J9 PREV VET MED JI Prev. Vet. Med. PD AUG 1 PY 2012 VL 105 IS 4 BP 265 EP 270 DI 10.1016/j.prevetmed.2012.01.007 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 976KX UT WOS:000306584600003 PM 22305661 ER PT J AU Ullah, A Sethumadhavan, K Grimm, C Shockey, J AF Ullah, Abul Sethumadhavan, Kandan Grimm, Casey Shockey, Jay TI Purification and characterization of a soluble diacylglycerol-3-phosphate phosphohydrolase, EC 3.1.3.4, from bottle gourd Lagenaria siceraria SO PROTEIN SCIENCE LA English DT Meeting Abstract CT 26th Annual Symposium of the Protein-Society CY AUG 05-08, 2012 CL San Diego, CA SP Prot Soc, Genentech, BioSilta, Aviv Biomed, Inc, Jasco, Purtein, Wyatt Technol Corp, Emerald Biosyst C1 [Ullah, Abul; Sethumadhavan, Kandan; Grimm, Casey; Shockey, Jay] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD AUG PY 2012 VL 21 SU 1 SI SI BP 148 EP 149 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 982CR UT WOS:000307019800240 ER PT J AU Nicholson, E Vrentas, C Greenlee, J AF Nicholson, Eric Vrentas, Catherine Greenlee, Justin TI Stability of PrPSc from cattle inoculated with different BSE strains SO PROTEIN SCIENCE LA English DT Meeting Abstract CT 26th Annual Symposium of the Protein-Society CY AUG 05-08, 2012 CL San Diego, CA SP Prot Soc, Genentech, BioSilta, Aviv Biomed, Inc, Jasco, Purtein, Wyatt Technol Corp, Emerald Biosyst C1 [Nicholson, Eric; Vrentas, Catherine; Greenlee, Justin] Agr Res Serv, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD AUG PY 2012 VL 21 SU 1 SI SI BP 210 EP 210 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 982CR UT WOS:000307019800392 ER PT J AU Vrentas, C Nicholson, E Greenlee, J AF Vrentas, Catherine Nicholson, Eric Greenlee, Justin TI Use of an ELISA Stability Assay to examine host genotype, PrPSc stability, and incubation time relationships in US livestock prion strains SO PROTEIN SCIENCE LA English DT Meeting Abstract CT 26th Annual Symposium of the Protein-Society CY AUG 05-08, 2012 CL San Diego, CA SP Prot Soc, Genentech, BioSilta, Aviv Biomed, Inc, Jasco, Purtein, Wyatt Technol Corp, Emerald Biosyst C1 [Vrentas, Catherine; Nicholson, Eric; Greenlee, Justin] USDA, Natl Anim Dis Ctr, Ames, IA 50021 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD AUG PY 2012 VL 21 SU 1 SI SI BP 211 EP 211 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 982CR UT WOS:000307019800393 ER PT J AU Lou, FF Huang, PW Neetoo, H Gurtler, JB Niemira, BA Chen, HQ Jiang, X Li, JR AF Lou, Fangfei Huang, Pengwei Neetoo, Hudaa Gurtler, Joshua B. Niemira, Brendan A. Chen, Haiqiang Jiang, Xi Li, Jianrong TI High-Pressure Inactivation of Human Norovirus Virus-Like Particles Provides Evidence that the Capsid of Human Norovirus Is Highly Pressure Resistant SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HIGH HYDROSTATIC-PRESSURE; HEPATITIS-A VIRUS; VESICULAR STOMATITIS-VIRUS; NORWALK VIRUS; MURINE NOROVIRUS; FELINE CALICIVIRUS; SURROGATE; TEMPERATURE; OYSTERS; PROTEIN AB Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food-and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, the effectiveness of HPP in inactivating human NoV remains poorly understood. In this study, we evaluated the effectiveness of HPP in disrupting the capsid of human NoV based on the structural and functional integrity of virus-like particles (VLPs) and histo-blood group antigen (HBGA) receptor binding assays. We found that pressurization at 500 to 600 MPa for 2 min, a pressure level that completely inactivates murine norovirus and feline calicivirus, was not sufficient to disrupt the structure and function of human NoV VLPs, even with a holding time of 60 min. Degradation of VLPs increased commensurate with increasing pressure levels more than increasing time. The times required for complete disruption of human NoV VLPs at 700, 800, and 900 MPa were 45, 15, and 2 min, respectively. Human NoV VLPs were more resistant to HPP in their ability to bind type A than type B and O HBGAs. Additionally, the 23-nm VLPs appeared to be much more stable than the 38-nm VLPs. Taken together, our results demonstrated that the human NoV capsid is highly resistant to HPP. While human NoV VLPs may not be fully representative of viable human NoV, destruction of the VLP capsid is highly suggestive of a typical response for viable human NoV. C1 [Lou, Fangfei; Li, Jianrong] Ohio State Univ, Dept Food Sci & Technol, Coll Food Agr & Environm Sci, Columbus, OH 43210 USA. [Li, Jianrong] Ohio State Univ, Coll Publ Hlth, Div Environm Hlth Sci, Columbus, OH 43210 USA. [Huang, Pengwei; Jiang, Xi] Cincinnati Childrens Hosp Med Ctr, Div Infect Dis, Cincinnati, OH USA. [Gurtler, Joshua B.; Niemira, Brendan A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Neetoo, Hudaa; Chen, Haiqiang] Univ Delaware, Dept Anim & Food Sci, Newark, DE USA. RP Li, JR (reprint author), Ohio State Univ, Dept Food Sci & Technol, Coll Food Agr & Environm Sci, Columbus, OH 43210 USA. EM li.926@osu.edu RI Li, Jianrong/E-3510-2011; Lou, Fangfei/J-9563-2014 OI Lou, Fangfei/0000-0002-9335-8209 FU USDA National Integrated Food Safety Initiative (NIFSI) [2010-01498]; USDA Agriculture and Food Research Initiative (AFRI) [2011-68003-30005]; USDA NIFA [2011-68003-30395] FX This study was supported by a special-emphasis grant (2010-01498) from the USDA National Integrated Food Safety Initiative (NIFSI) to J.L., H.C., and X.J., a food safety challenge grant (2011-68003-30005) from the USDA Agriculture and Food Research Initiative (AFRI) to H.C., J.L., X.J., J.B.G., and B.A.N., and a NoroCORE project grant (2011-68003-30395) from USDA NIFA to J.L. and X.J. NR 39 TC 15 Z9 15 U1 0 U2 22 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2012 VL 78 IS 15 BP 5320 EP 5327 DI 10.1128/AEM.00532-12 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 975ST UT WOS:000306532500035 PM 22635990 ER PT J AU Low, SA Weiler, S AF Low, Sarah A. Weiler, Stephan TI Employment Risk, Returns, and Entrepreneurship SO ECONOMIC DEVELOPMENT QUARTERLY LA English DT Article DE entrepreneurship; employment portfolio; self-employment; employment risk; establishment births ID REGIONAL INDUSTRIAL DIVERSIFICATION; FRONTIER PRODUCTION FUNCTION; GROWTH-INSTABILITY FRONTIER; FIRM FORMATION; DETERMINANTS; PORTFOLIO; STATE; SIZE; US AB Comparing local employment portfolios against entrepreneurship, this research finds that local wage and salary job market prospects shape incentives for potential entrepreneurs. Entrepreneurship may thus be more attractive in areas featuring high employment risk and/or low returns. This research contributes to the existing regional employment portfolio literature by using more disaggregated data at both the county and the commuting zone levels. Commuting zones in particular represent a broader spectrum of labor market agglomerations across both rural and urban areas to provide the most stringent and revealing tests of the interrelationship between local employment portfolios and the choice to pursue entrepreneurship. The authors find a U-shaped risk/return trade-off using employment variance and growth, consistent with the literature. They test their hypothesis with a model of regional entrepreneurship, incorporating the employment portfolio variables. This is the first known study to explore the hypothesized relationship between wage and salary employment portfolios and entrepreneurship, effectively synthesizing two previously disparate literatures. C1 [Low, Sarah A.] ERS, USDA, Farm & Rural Business Branch, Washington, DC 20250 USA. [Weiler, Stephan] Colorado State Univ, Ft Collins, CO 80523 USA. RP Low, SA (reprint author), ERS, USDA, Farm & Rural Business Branch, 1400 Independence Ave SW,MS-1800, Washington, DC 20250 USA. EM slow@ers.usda.gov NR 60 TC 6 Z9 6 U1 1 U2 18 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0891-2424 J9 ECON DEV Q JI Econ. Dev. Q. PD AUG PY 2012 VL 26 IS 3 BP 238 EP 251 DI 10.1177/0891242412452445 PG 14 WC Economics; Planning & Development; Urban Studies SC Business & Economics; Public Administration; Urban Studies GA 975QI UT WOS:000306525500004 ER PT J AU Azzi, A AF Azzi, Angelo TI In memory of Donald Nicholson SO IUBMB LIFE LA English DT Biographical-Item C1 Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Azzi, A (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM angelo.azzi@tufts.edu NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1521-6543 J9 IUBMB LIFE JI IUBMB Life PD AUG PY 2012 VL 64 IS 8 BP 659 EP 660 DI 10.1002/iub.1061 PG 2 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 974ZX UT WOS:000306477300001 ER PT J AU Blackwell, BF DeVault, TL Seamans, TW Lima, SL Baumhardt, P Fernandez-Juricic, E AF Blackwell, Bradley F. DeVault, Travis L. Seamans, Thomas W. Lima, Steven L. Baumhardt, Patrice Fernandez-Juricic, Esteban TI Exploiting avian vision with aircraft lighting to reduce bird strikes SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE aircraft lighting; airport; antipredator behaviour; avian vision; bird strike; Branta canadensis; human-wildlife interactions; sensory ecology ID HUMAN DISTURBANCE; COLOR; RESPONSES; ECOLOGY; GEESE; CONSERVATION; COLLISIONS; PATTERNS; BEHAVIOR; PREDATOR AB 1. Birdaircraft collisions (bird strikes) represent a substantial safety concern and financial burden to civil aviation world-wide. Despite an increase in the rate of damaging bird strikes, necessary steps to develop a mitigation method outside of the airport environment have not been empirically tested. 2. We assessed whether use of aircraft lighting might enhance detection of and reaction to the approach of an aircraft in flight by Canada geese Branta canadensis Linnaeus, a species responsible for a high rate of damaging bird strikes. We used a novel approach by estimating the visibility to the goose visual system of a standard radio-controlled (RC) aircraft (standard aircraft) exhibiting either a 2-Hz alternating pulse of two lights, or lights off; and another RC aircraft designed to mimic a raptor (predator model). We then exposed wing-clipped Canada geese to the approach of each aircraft and quantified behavioural responses to respective treatments. 3. Estimates of chromatic and achromatic contrasts indicated that the standard aircraft with lights on was more salient to the visual system of the Canada goose than with lights off or the predator model. 4. At individual and group levels, quicker alert responses were observed to the standard aircraft with lights compared with the lights off and predator model. Goose groups showed similar responses to approaches by the standard aircraft and the predator model, suggesting use of antipredator behaviour to avoid the aircraft. 5. Synthesis and applications. Understanding animal sensory ecology and associated behaviours can aid the development of methods exploiting certain behaviours to reduce negative humanwildlife interactions. For example, reducing the frequency of bird strikes requires the integration of wildlife management efforts within and outside of the airport environment that target species resource use and response to disturbance, with mitigation techniques focused on the aircraft. Moreover, the design of aircraft lighting systems to enhance detection and avoidance by birds is contingent upon understanding avian visual ecology and behaviour. Based on spectral sensitivity in Canada geese, aircraft-mounted lights that peak in the ultraviolet/violet range (380400 nm) are likely to produce the maximal behavioural effect. C1 [Blackwell, Bradley F.; DeVault, Travis L.; Seamans, Thomas W.] USDA APHIS WS Natl Wildlife Res Ctr, Ohio Field Stn, Sandusky, OH 44870 USA. [Lima, Steven L.] Indiana State Univ, Dept Biol, Terre Haute, IN 47809 USA. [Baumhardt, Patrice; Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. RP Blackwell, BF (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM bradley.f.blackwell@aphis.usda.gov FU U.S. Department of Agriculture/Animal and Plant Health Inspection Service/Wildlife Services' National Wildlife Research Center, Purdue University; Indiana State University; U.S. Federal Aviation Administration [DTFACT-04-X-90003]; Precise Flight, Inc., Bend, OR, USA; Premises Control, Parma, OH, USA FX Our research was supported by the U.S. Department of Agriculture/Animal and Plant Health Inspection Service/Wildlife Services' National Wildlife Research Center, Purdue University, Indiana State University, the U.S. Federal Aviation Administration under agreement DTFACT-04-X-90003, Precise Flight, Inc., Bend, OR, USA, and Premises Control, Parma, OH, USA. We deeply thank Paolo Iori, inventor of the Falco Robot GBRS (c), for his suggestions during the study. We also thank B. Buckingham, M. Conger, M. Majoy, S. Philiben, E. Poggiali, D. Steyer and L. Tyson for their assistance during our behavioural experiments. E. Poggiali and M. Stapanian provided reviews of earlier drafts of this manuscript. NR 39 TC 17 Z9 18 U1 97 U2 1857 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2012 VL 49 IS 4 BP 758 EP 766 DI 10.1111/j.1365-2664.2012.02165.x PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 974ZU UT WOS:000306477000002 ER PT J AU Schmerge, D Francis, O Henton, J Ingles, D Jones, D Kennedy, J Krauterbluth, K Liard, J Newell, D Sands, R Schiel, A Silliker, J van Westrum, D AF Schmerge, D. Francis, O. Henton, J. Ingles, D. Jones, D. Kennedy, J. Krauterbluth, K. Liard, J. Newell, D. Sands, R. Schiel, A. Silliker, J. van Westrum, D. TI Results of the first North American comparison of absolute gravimeters, NACAG-2010 SO JOURNAL OF GEODESY LA English DT Article DE Absolute gravimetry; Gravimeter; GRAV-D; Comparison; Offset ID GRAVITY METERS AB The first North American Comparison of absolute gravimeters (NACAG-2010) was hosted by the National Oceanic and Atmospheric Administration at its newly renovated Table Mountain Geophysical Observatory (TMGO) north of Boulder, Colorado, in October 2010. NACAG-2010 and the renovation of TMGO are part of NGS's GRAV-D project (Gravity for the Redefinition of the American Vertical Datum). Nine absolute gravimeters from three countries participated in the comparison. Before the comparison, the gravimeter operators agreed to a protocol describing the strategy to measure, calculate, and present the results. Nine sites were used to measure the free-fall acceleration of g. Each gravimeter measured the value of g at a subset of three of the sites, for a total set of 27 g-values for the comparison. The absolute gravimeters agree with one another with a standard deviation of 1.6 mu Gal (1 Gal a parts per thousand 1 cm s (-2)). The minimum and maximum offsets are -2.8 and 2.7 mu Gal. This is an excellent agreement and can be attributed to multiple factors, including gravimeters that were in good working order, good operators, a quiet observatory, and a short duration time for the experiment. These results can be used to standardize gravity surveys internationally. C1 [Schmerge, D.] US Forest Serv, Juneau, AK USA. [Francis, O.] Univ Luxembourg, Luxembourg, Luxembourg. [Henton, J.] Nat Resources Canada, Sidney, BC, Canada. [Ingles, D.] Natl Res Council Canada, Ottawa, ON, Canada. [Jones, D.; Schiel, A.; van Westrum, D.] Microg LaCoste Inc, Lafayette, CO USA. [Kennedy, J.] US Geol Survey, Tucson, AZ USA. [Krauterbluth, K.; Sands, R.] Natl Geospatial Intelligence Agcy, Patrick AFB, FL USA. [Liard, J.; Silliker, J.] Nat Resources Canada, Ottawa, ON, Canada. [Newell, D.] Natl Inst Stand & Technol, Gaithersburg, MD 20899 USA. RP Schmerge, D (reprint author), US Forest Serv, Juneau, AK USA. EM david.schmerge@yahoo.com NR 14 TC 8 Z9 9 U1 1 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0949-7714 J9 J GEODESY JI J. Geodesy PD AUG PY 2012 VL 86 IS 8 BP 591 EP 596 DI 10.1007/s00190-011-0539-y PG 6 WC Geochemistry & Geophysics; Remote Sensing SC Geochemistry & Geophysics; Remote Sensing GA 975ET UT WOS:000306490700002 ER PT J AU Lee, J Rudell, DR Davies, PJ Watkins, CB AF Lee, Jinwook Rudell, David R. Davies, Peter J. Watkins, Christopher B. TI Metabolic changes in 1-methylcyclopropene (1-MCP)-treated 'Empire' apple fruit during storage SO METABOLOMICS LA English DT Article DE Metabolomics; Partial least squares analysis (PLS); GC-MS; Sorbitol; GABA; Amino acids; Volatiles; Phenolic compounds; Flesh browning ID CONTROLLED-ATMOSPHERE STORAGE; CHROMATOGRAPHY-MASS SPECTROMETRY; CARBON-DIOXIDE INJURY; PINK-LADY-APPLES; VOLATILE PRODUCTION; FUJI APPLES; SUPERFICIAL SCALD; DELICIOUS APPLES; COLD-STORAGE; GALA APPLES AB 'Empire' apple fruit are more susceptible to flesh browning at 3.3A degrees C if treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. To better understand the metabolic changes associated with this browning, untargeted metabolic profiling with partial least squares analysis has been used to visualize changes in metabolic profile during hypoxic controlled atmosphere (CA) storage, ethylene insensitivity, and disorder development. Overall, most carbohydrates and organic acids were not appreciably affected, but the levels of amino acids and volatile metabolites were significantly affected, by 1-MCP treatment. Sorbitol and levels of some amino acids were elevated towards the end of storage in 1-MCP treated fruit. CA storage reduced the levels of many volatile components and 1-MCP reduced these levels further. Additionally multiple metabolites were associated with the development of flesh browning symptoms. Unlike other volatile compounds, methanol levels gradually increased with storage duration, regardless of 1-MCP treatment, while 1-MCP decreased ethanol production. Results reveal metabolic changes during storage that may be associated with development of flesh browning symptoms. C1 [Lee, Jinwook; Watkins, Christopher B.] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Rudell, David R.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Davies, Peter J.] Cornell Univ, Dept Plant Biol & Hort, Ithaca, NY 14853 USA. RP Watkins, CB (reprint author), Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. EM cbw3@cornell.edu FU Federal Formula Funds [NE1036]; New York Apple Research and Development Program; AgroFresh, Inc.; Department of Horticulture assistantship FX We thank Jackie Nock at the Department of Horticulture, Cornell University, and David Buchanan and Rachel Leisso at USDA-ARS, Tree Fruit Research Laboratory, for their assistance. This research was partly supported by Federal Formula Funds NE1036, the New York Apple Research and Development Program, and AgroFresh, Inc. Jinwook Lee was supported by a Department of Horticulture assistantship. NR 77 TC 34 Z9 38 U1 1 U2 54 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 J9 METABOLOMICS JI Metabolomics PD AUG PY 2012 VL 8 IS 4 BP 742 EP 753 DI 10.1007/s11306-011-0373-5 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 975ZF UT WOS:000306549600020 ER PT J AU Wicklein, HF Ollinger, SV Martin, ME Hollinger, DY Lepine, LC Day, MC Bartlett, MK Richardson, AD Norby, RJ AF Wicklein, Haley F. Ollinger, Scott V. Martin, Mary E. Hollinger, David Y. Lepine, Lucie C. Day, Michelle C. Bartlett, Megan K. Richardson, Andrew D. Norby, Richard J. TI Variation in foliar nitrogen and albedo in response to nitrogen fertilization and elevated CO2 SO OECOLOGIA LA English DT Article DE Albedo; Nitrogen; Leaf structure; Nitrogen fertilization; Free air CO2 enrichment ID LEAF OPTICAL-PROPERTIES; MESOPHYLL SURFACE-AREA; CANOPY REFLECTANCE; SAIL MODEL; SWEETGUM PLANTATION; PHOTOSYNTHETIC RATE; FOREST CANOPY; DRY MASS; LEAVES; LIGHT AB Foliar nitrogen has been shown to be positively correlated with midsummer canopy albedo and canopy near infrared (NIR) reflectance over a broad range of plant functional types (e.g., forests, grasslands, and agricultural lands). To date, the mechanism(s) driving the nitrogen-albedo relationship have not been established, and it is unknown whether factors affecting nitrogen availability will also influence albedo. To address these questions, we examined variation in foliar nitrogen in relation to leaf spectral properties, leaf mass per unit area, and leaf water content for three deciduous species subjected to either nitrogen (Harvard Forest, MA, and Oak Ridge, TN) or CO2 fertilization (Oak Ridge, TN). At Oak Ridge, we also obtained canopy reflectance data from the airborne visible/infrared imaging spectrometer (AVIRIS) to examine whether canopy-level spectral responses were consistent with leaf-level results. At the leaf level, results showed no differences in reflectance or transmittance between CO2 or nitrogen treatments, despite significant changes in foliar nitrogen. Contrary to our expectations, there was a significant, but negative, relationship between foliar nitrogen and leaf albedo, a relationship that held for both full spectrum leaf albedo as well as leaf albedo in the NIR region alone. In contrast, remote sensing data indicated an increase in canopy NIR reflectance with nitrogen fertilization. Collectively, these results suggest that altered nitrogen availability can affect canopy albedo, albeit by mechanisms that involve canopy-level processes rather than changes in leaf-level reflectance. C1 [Wicklein, Haley F.; Ollinger, Scott V.; Martin, Mary E.; Lepine, Lucie C.; Day, Michelle C.] Univ New Hampshire, Complex Syst Res Ctr, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. [Hollinger, David Y.] USDA Forest Service, No Res Stn, Durham, NH 03824 USA. [Bartlett, Megan K.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Herbarium, Cambridge, MA 02138 USA. [Norby, Richard J.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. RP Wicklein, HF (reprint author), Univ New Hampshire, Complex Syst Res Ctr, Inst Study Earth Oceans & Space, Morse Hall,8 Coll Rd, Durham, NH 03824 USA. EM hwicklein@abermail.sr.unh.edu RI Norby, Richard/C-1773-2012; Hollinger, David/G-7185-2012; Richardson, Andrew/F-5691-2011; Ollinger, Scott/N-3380-2014; OI Norby, Richard/0000-0002-0238-9828; Richardson, Andrew/0000-0002-0148-6714; Ollinger, Scott/0000-0001-6226-1431; Lepine, Lucie/0000-0003-1028-2534; Martin, Mary/0000-0003-1791-5584 FU North American Carbon Program (NACP) NASA's Terrestrial Ecology and Carbon Cycle Science Programs; US Department of Energy, Office of Science, Biological and Environmental Research Program; National Science Foundation [DBI-0452254] FX We thank G. James Collatz for helpful comments on a draft of this manuscript, Rob Braswell for providing the SAIL-2 model code, and Richard Norby, Colleen Iversen, and JeVery Warren for support at ORNL. We are indebted to Michael Eastwood, ER-2 pilots Denis Steel, Tim Williams, and the rest of the AVIRIS team for aircraft data acquisition. This work was funded by a grant from the North American Carbon Program (NACP) NASA's Terrestrial Ecology and Carbon Cycle Science Programs and a graduate fellowship provided by the Research and Discover program. The ORNL FACE experiment was supported by the US Department of Energy, Office of Science, Biological and Environmental Research Program. A.D.R. and M.K.B. acknowledge support, through the Harvard Forest REU program, from the National Science Foundation (Grant DBI-0452254). NR 47 TC 12 Z9 12 U1 2 U2 44 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 J9 OECOLOGIA JI Oecologia PD AUG PY 2012 VL 169 IS 4 BP 915 EP 925 DI 10.1007/s00442-012-2263-6 PG 11 WC Ecology SC Environmental Sciences & Ecology GA 975GA UT WOS:000306494800006 PM 22294028 ER PT J AU Gao, YL Lei, ZR Reitz, SR AF Gao, Yulin Lei, Zhongren Reitz, Stuart R. TI Western flower thrips resistance to insecticides: detection, mechanisms and management strategies SO PEST MANAGEMENT SCIENCE LA English DT Review DE thrips; insecticide resistance management; Frankliniella occidentalis; IPM ID FRANKLINIELLA-OCCIDENTALIS PERGANDE; ECONOMIC INJURY LEVELS; IN-FIELD POPULATIONS; THYSANOPTERA-THRIPIDAE; PEST-MANAGEMENT; METHIOCARB RESISTANCE; INTEGRATED MANAGEMENT; SPINOSAD RESISTANCE; LIFE-HISTORY; GREENHOUSE AB Insecticide resistance continues to be one of the most important issues facing agricultural production. The challenges in insecticide resistance and its management are exemplified by the situation with the western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). This highly invasive pest has a great propensity for developing insecticide resistance because of its biological attributes, and cases of resistance to most classes of insecticides used for its management have been detected. To combat insecticide resistance in the western flower thrips, several insecticide resistance management (IRM) programs have been developed around the world, and these are discussed. Successful programs rely on non-insecticidal tactics, such as biological and cultural controls and host plant resistance, to reduce population pressures, rotations among insecticides of different mode of action classes to conserve insecticide efficacy, resistance monitoring, sampling to determine the need for insecticide applications and education to assure proper implementation. More judicious insecticide use is possible with the development of well-founded economic thresholds for more cropping systems. While growers will continue to rely on insecticides as part of western-flower-thrips- and thrips-transmitted virus management, more effective management of these pests will be achieved by considering their management in the context of overall integrated pest management, with IRM being a key component of those comprehensive programs. Copyright (c) 2012 Society of Chemical Industry C1 [Gao, Yulin; Lei, Zhongren] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. [Reitz, Stuart R.] Agr Res Serv, Ctr Med Agr & Vet Entomol, USDA, Tallahassee, FL USA. RP Gao, YL (reprint author), Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. EM ylgao@ippcaas.cn; zrlei@ippcaas.cn; stuart.reitz@ars.usda.gov FU China Agriculture Research System [CARS-25-B-07] FX The authors are grateful to Brenda Oppert (USDA-ARS-CGAHR) and two anonymous reviewers for their helpful comments on previous versions of the manuscript. This research was supported by the China Agriculture Research System (CARS-25-B-07). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 99 TC 37 Z9 42 U1 3 U2 77 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD AUG PY 2012 VL 68 IS 8 BP 1111 EP 1121 DI 10.1002/ps.3305 PG 11 WC Agronomy; Entomology SC Agriculture; Entomology GA 975GK UT WOS:000306496300002 PM 22566175 ER PT J AU Tremblay, A Hosseini, P Li, S Alkharouf, NW Matthews, BF AF Tremblay, A. Hosseini, P. Li, S. Alkharouf, N. W. Matthews, B. F. TI Identification of genes expressed by Phakopsora pachyrhizi, the pathogen causing soybean rust, at a late stage of infection of susceptible soybean leaves SO PLANT PATHOLOGY LA English DT Article DE deep sequencing; gene expression; Glycine max; Phakopsora pachyrhizi; soybean; soybean rust ID COMPONENT FATTY ACIDS; UDP-GALACTOPYRANOSE MUTASE; TRANSFER-RNA SYNTHETASE; PLANT RUSTS; ASPERGILLUS-NIDULANS; CONFERS RESISTANCE; CANDIDA-ALBICANS; BAY 10-8888; FUNGI; SPORES AB Soybean is one of the top five agricultural products in the United States and is highly susceptible to Phakopsora pachyrhizi, an exotic obligate biotrophic fungus. The little amount of genomic information about P. pachyrhizi limits understanding of the soybeansoybean rust pathogen interaction and the possibility of engineering resistance to this pathogen in soybean. Illumina mRNA-Seq analysis revealed P. pachyrhizi genes expressed during a biotrophic interaction between P. pachyrhizi and soybean during fungal sporulation 10 days after inoculation. Approximately 2.4 million DNA sequences representing portions of potential P. pachyrhizi genes were assembled into 32 940 contigs that were used to search against expressed sequence tag (EST), protein and conserved domain databases. About 7500 contigs represent newly discovered P. pachyrhizi sequences. Of these, 527 shared similarity to genes encoding fungal proteins involved in different metabolic pathways such as galactose and glycogen metabolism, glycolysis, the citrate cycle, fatty acid metabolism, amino acid metabolism, proteolysis, protein synthesis, cell cycle division and mitosis, and cell wall biogenesis. Almost 7000 potential P. pachyrhizi genes are still of unknown function. Such information may be useful in the development of new methods of broadening resistance of soybean to P. pachyrhizi, including the silencing of important P. pachyrhizi genes, and also to understand the molecular basis of soybeanP. pachyrhizi interactions. C1 [Tremblay, A.; Hosseini, P.; Matthews, B. F.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Li, S.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Alkharouf, N. W.] Towson Univ, Towson, MD 21252 USA. RP Tremblay, A (reprint author), USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. EM arianne@umbc.edu FU United Soybean Board [T0258] FX The authors thank Alicia Beavers for her excellent technical assistance, and Richard Jones, Dilip Lakshman, Eric Brewer, and Richard Joost for their careful critical review of the manuscript. The authors gratefully acknowledge support from United Soybean Board project number T0258. NR 60 TC 7 Z9 7 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 J9 PLANT PATHOL JI Plant Pathol. PD AUG PY 2012 VL 61 IS 4 BP 773 EP 786 DI 10.1111/j.1365-3059.2011.02550.x PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 975GD UT WOS:000306495300016 ER PT J AU Zhang, XH Pan, HY Bai, GH AF Zhang, Xianghui Pan, Hongyu Bai, Guihua TI Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID LENGTH POLYMORPHISM MARKERS; TRITICUM-AESTIVUM L.; SPRING WHEAT; WINTER-WHEAT; SCAB RESISTANCE; DEOXYNIVALENOL ACCUMULATION; SSR MARKERS; MAJOR QTL; MICROSATELLITE; POPULATION AB Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease that can significantly reduce grain yield and quality. Deployment of quantitative trait loci (QTLs) for FHB resistance in commercial cultivars has been the most effective approach for minimizing the disease losses. 'Baishanyuehuang' is a highly FHB-resistant landrace from China. Recombinant inbred lines (RILs) developed from a cross of 'Baishanyuehuang' and 'Jagger' were evaluated for FHB resistance in three greenhouse experiments in 2010 and 2011 by single-floret inoculation. Percentage of symptomatic spikelets in an inoculated spike was recorded 18 days post-inoculation. The RIL population was screened with 251 polymorphic simple sequence repeats. Four QTLs were associated with FHB resistance and mapped on three chromosomes. Two QTLs were located on the short arm of chromosome 3B (3BS) with one in distal of 3BS and another near centromere (3BSc), designated as Qfhb.hwwg-3BSc. The QTL in the distal of 3BS is flanked by Xgwm533 and Xgwm493, thus corresponds to Fhb1. This QTL explained up to 15.7 % of phenotypic variation. Qfhb.hwwg-3BSc flanked by Xwmc307 and Xgwwm566 showed a smaller effect than Fhb1 and explained up to 8.5 % of phenotypic variation. The other two QTLs were located on 3A, designated as Qfhb.hwwg-3A, and 5A, designated as Qfhb.hwwg-5A. Qfhb.hwwg-3A was flanked by Xwmc651 and Xbarc356 and explained 4.8-7.5 % phenotypic variation, and Qfhb.hwwg-5A was flanked by markers Xgwm186 and Xbarc141, detected in only one experiment, and explained 4.5 % phenotypic variation for FHB resistance. 'Baishanyuehuang' carried all resistance alleles of the four QTL. Qfhb.hwwg-3BSc and Qfhb.hwwg-3A were new QTLs in 'Baishanyuehuang'. 'Baishanyuehuang' carries a combination of QTLs from different sources and can be a new source of parent to pyramid FHB-resistant QTLs for improving FHB resistance in wheat. C1 [Zhang, Xianghui; Pan, Hongyu] Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China. [Zhang, Xianghui; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Pan, HY (reprint author), Jilin Univ, Coll Plant Sci, Changchun 130062, Jilin, Peoples R China. EM panhongyu@jlu.edu.cn; guihua.bai@ars.usda.gov FU National Research Initiative from USDA National Institute of Food and Agriculture [2011-68002-30029]; USDA Wheat and Barley Scab Initiative; China Scholarship Council FX This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and USDA Wheat and Barley Scab Initiative. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This is contribution No. 12-093-J from the Kansas Agricultural Experiment Station, Manhattan, KS. X. Zhang was partially supported by China Scholarship Council. NR 42 TC 7 Z9 9 U1 3 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2012 VL 125 IS 3 BP 495 EP 502 DI 10.1007/s00122-012-1848-0 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 974KC UT WOS:000306432700007 PM 22454145 ER PT J AU Pham, AT Shannon, JG Bilyeu, KD AF Anh-Tung Pham Shannon, J. Grover Bilyeu, Kristin D. TI Combinations of mutant FAD2 and FAD3 genes to produce high oleic acid and low linolenic acid soybean oil SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID FATTY-ACIDS; GROWTH TEMPERATURE; DESATURASE GENES; SEED OIL; TRAITS; IDENTIFICATION; PERFORMANCE; STABILITY; GMFAD2-1A; SELECTION AB High oleic acid soybeans were produced by combining mutant FAD2-1A and FAD2-1B genes. Despite having a high oleic acid content, the linolenic acid content of these soybeans was in the range of 4-6 %, which may be high enough to cause oxidative instability of the oil. Therefore, a study was conducted to incorporate one or two mutant FAD3 genes into the high oleic acid background to further reduce the linolenic acid content. As a result, soybean lines with high oleic acid and low linolenic acid (HOLL) content were produced using different sources of mutant FAD2-1A genes. While oleic acid content of these HOLL lines was stable across two testing environments, the reduction of linolenic acid content varied depending on the number of mutant FAD3 genes combined with mutant FAD2-1 genes, on the severity of mutation in the FAD2-1A gene, and on the testing environment. Combination of two mutant FAD2-1 genes and one mutant FAD3 gene resulted in less than 2 % linolenic acid content in Portageville, Missouri (MO) while four mutant genes were needed to achieve the same linolenic acid in Columbia, MO. This study generated non-transgenic soybeans with the highest oleic acid content and lowest linolenic acid content reported to date, offering a unique alternative to produce a fatty acid profile similar to olive oil. C1 [Bilyeu, Kristin D.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Anh-Tung Pham] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Shannon, J. Grover] Univ Missouri, Univ Missouri Delta Res Ctr, Div Plant Sci, Portageville, MO 63873 USA. RP Bilyeu, KD (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, 110 Waters Hall, Columbia, MO 65211 USA. EM kristin.bilyeu@ars.usda.gov NR 53 TC 22 Z9 29 U1 4 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2012 VL 125 IS 3 BP 503 EP 515 DI 10.1007/s00122-012-1849-z PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 974KC UT WOS:000306432700008 PM 22476873 ER PT J AU Iqbal, MJ Mamidi, S Ahsan, R Kianian, SF Coyne, CJ Hamama, AA Narina, SS Bhardwaj, HL AF Iqbal, Muhammad Javed Mamidi, Sujan Ahsan, Rubina Kianian, Shahryar F. Coyne, Clarice J. Hamama, Anwar A. Narina, Satya S. Bhardwaj, Harbans L. TI Population structure and linkage disequilibrium in Lupinus albus L. germplasm and its implication for association mapping SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID SPATIAL GENETIC-STRUCTURE; MULTILOCUS GENOTYPE DATA; MAYS SSP PARVIGLUMIS; WHITE LUPIN; ARABIDOPSIS-THALIANA; COMPLEX TRAITS; MODEL; AFLP; IDENTIFICATION; ARCHITECTURE AB White lupin (Lupinus albus L.) has been around since 300 B.C. and is recognized for its ability to grow on poor soils and application as green manure in addition to seed harvest. The seed has very high levels of protein (33-47 %) and oil (6-13 %). It also has many secondary metabolites that are potentially of nutraceutical value to animals and humans. Despite such a great potential, lupins role in modern agriculture began only in the twentieth century. Although a large collection of Lupinus germplasm accessions is available worldwide, rarely have they been genetically characterized. Additionally, scarce genomic resources in terms of recombinant populations and genome information have been generated for L. albus. With the advancement in association mapping methods, the natural populations have the potential to replace the recombinant populations in gene mapping and marker-trait associations. Therefore, we studied the genetic similarity, population structure and marker-trait association in a USDA germplasm collection for their current and future application in this crop improvement. A total of 122 PI (Plant Inventory) lines were screened with 18 AFLP primer pairs that generated 2,277 fragments. A subset of 892 polymorphic markers with MAF > 0.05 (minor allele frequency) were used for association mapping. The cluster analysis failed to group accessions on the basis of their passport information, and a weak structure and low linkage disequilibrium (LD) were observed indicating the usefulness of the collection for association mapping. Moreover, we were also able to identify two markers (a p value of 1.53 x 10(-4) and 2.3 x 10(-4)) that explained 22.69 and 20.5 % of seed weight variation determined using R (LR) (2) . The implications of lack of geographic clustering, population structure, low LD and the ability of AFLP to map seed weight trait using association mapping and the usefulness of the PI collections in breeding programs are discussed. C1 [Iqbal, Muhammad Javed; Mamidi, Sujan; Kianian, Shahryar F.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ahsan, Rubina] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58108 USA. [Hamama, Anwar A.; Narina, Satya S.; Bhardwaj, Harbans L.] Virginia State Univ, Agr Res Stn, Petersburg, VA 23806 USA. [Coyne, Clarice J.] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Iqbal, MJ (reprint author), N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM muhammad.iqbal@ndsu.edu; hbhardwj@vsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU USDA-NIFA [1890] FX This project was supported by USDA-NIFA-1890 Institutions Capacity Building Grants Program funds allocated to Virginia State University (H. L. Bhardwaj as PI/PD). The AFLP lab work was done at Institute for Advanced Learning and Research, Danville, VA by MJI and RA. Contribution of Virginia State University, Agricultural Research Station Journal Article Series Number 284. The use of any trade names or vendors does not imply approval to the exclusion of other products or vendors that may also be suitable. NR 69 TC 9 Z9 9 U1 0 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2012 VL 125 IS 3 BP 517 EP 530 DI 10.1007/s00122-012-1850-6 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 974KC UT WOS:000306432700009 PM 22454146 ER PT J AU De Steven, D Gramling, JM AF De Steven, Diane Gramling, Joel M. TI Diverse Characteristics of Wetlands Restored under the Wetlands Reserve Program in the Southeastern United States SO WETLANDS LA English DT Article DE Coastal Plain region, USA; Conservation Effects Assessment Project (CEAP); Ecosystem services; HGM; Wetland restoration; Wetlands Reserve Program ID RIVER ALLUVIAL VALLEY; CONSERVATION PRACTICES; ECOSYSTEM SERVICES; MANAGEMENT; RESTORATION; SUCCESS; REGION AB The Wetlands Reserve Program (WRP) restores converted or degraded wetlands on private working lands; however, the nature and outcomes of such efforts are undocumented in the Southeastern U.S. Identification of wetland types is needed to assess the program's conservation benefits, because ecological functions differ with hydrogeomorphic (HGM) type. We reviewed > 100 WRP projects across the Southeast Piedmont-Coastal Plain to characterize their wetland types and to evaluate whether restoration practices favored original or modified functions. The projects encompassed four HGM types and diverse pre-restoration conditions. Nearly half were converted wetlands retired from active agriculture; the remainder were either drained vegetated wetlands or forested bottomlands degraded by timber harvest. Hydrology-repair practices varied by wetland type and prior condition, with differing functional implications. Depressions and flats typically were restored, whereas low-order riparian sites and prior-agriculture floodplains were often modified to enhance water retention. Timber-harvested floodplains were restored by removing barriers to water flow and biotic connectivity. Vegetation restoration was generally passive, but tree planting was frequent on prior-agriculture sites. Field surveys suggested that most projects had positive indicators of wetland hydrology, vegetation, and faunal use. The variety of Southeastern WRP wetlands has implications for ecosystem services at local and landscape scales. C1 [De Steven, Diane] US Forest Serv, So Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. [Gramling, Joel M.] Citadel, Dept Biol, Charleston, SC 29409 USA. RP De Steven, D (reprint author), US Forest Serv, So Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. EM ddesteven@fs.fed.us FU USDA NRCS; U.S. Forest Service Southern Research Station; USFS FX We greatly appreciated the support of CEAP-Wetlands Science Coordinator Diane Eckles and the generous cooperation of NRCS State and Field Office staffs, especially WRP Coordinators Glenn Sandifer (SC), Mike Oliver (MS), and Keith Wooster (GA). We also thank the landowners for access to their WRP sites. Steven Hughes assisted in field work and data compilation, with additional help from Kristie Burr, Cody Clark, Anne Cubeta, and Kristi Wharton. Editorial and statistical reviews were provided by Kay Kirkman, Rebecca Sharitz, Ray Souter, and Tom Dell. The study was funded through the USDA NRCS, the U.S. Forest Service Southern Research Station, and a USFS Joint Venture Agreement with The Citadel Graduate College. NR 38 TC 6 Z9 6 U1 3 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD AUG PY 2012 VL 32 IS 4 BP 593 EP 604 DI 10.1007/s13157-012-0303-y PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 976ON UT WOS:000306594300001 ER PT J AU Brooks, RT Eggert, SL Nislow, KH Kolka, RK Chen, CY Ward, DM AF Brooks, Robert T. Eggert, Susan L. Nislow, Keith H. Kolka, Randall K. Chen, Celia Y. Ward, Darren M. TI Preliminary Assessment of Mercury Accumulation in Massachusetts and Minnesota Seasonal Forest Pools SO WETLANDS LA English DT Article DE Bioaccumulation; Methylmercury; Seasonal forest pools; Total mercury; Vernal pools ID NORTHEASTERN UNITED-STATES; ACADIA NATIONAL-PARK; NORTH-AMERICA; FOOD WEBS; EMBRYONIC MORTALITY; ACID PRECIPITATION; LARVAL AMPHIBIANS; CANOPY COVER; USA; BIOACCUMULATION AB Seasonal forest pools (SFPs) are common, widespread, and provide critical habitat for amphibians and invertebrates. The ephemeral hydrology of SFPs has been identified as an important factor in the production of biologically active methylmercury (MeHg). To investigate mercury (Hg) in SFPs, we collected water, fine benthic organic matter (FBOM), detrital materials, and faunal samples from 10 pools in Massachusetts and Minnesota, across a range of basin morphometry, water chemistry, canopy closure, and hydroperiod. Total Hg (THg) and MeHg concentrations were comparable to other small, lentic waters. MeHg was found in greater concentrations in fauna than in periphyton, detrital items, or FBOM. In spite of consistently lower pool acidity and higher THg deposition in Massachusetts, we found no significant differences in Hg concentrations between states. No significant relationship was found between MeHg concentration and canopy closure. A weak, positive relationship was identified between pool hydroperiod and the bioaccumulation of THg, suggesting that longer exposure may be a factor in increased Hg concentrations in basal resources, which is then propagated into consumer trophic levels. Further work on the mechanisms determining Hg trophic transfer and bioaccumulation is required to fully understand the influence of hydrogeochemical and landscape influences on Hg dynamics in SFPs. C1 [Brooks, Robert T.; Nislow, Keith H.] US Forest Serv, No Res Stn, Amherst, MA 01003 USA. [Eggert, Susan L.; Kolka, Randall K.] US Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Chen, Celia Y.; Ward, Darren M.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03775 USA. RP Brooks, RT (reprint author), US Forest Serv, No Res Stn, Amherst, MA 01003 USA. EM rtbrooks@fs.fed.us RI Ward, Darren/F-1203-2010 OI Ward, Darren/0000-0002-0049-5299 FU U.S. Forest Service Eastern Region Renewable Resources; National Institutes of Health from the National Institute of Environmental Health Sciences [P42 ESO7373] FX T. Humphrey provided critical support for the collection of Massachusetts samples and field data. E. Moreno processed the mercury samples and A. Baker and B. Jackson analyzed mercury speciation. The research was partially supported by the U.S. Forest Service Eastern Region Renewable Resources (S. L. Eggert) and the National Institutes of Health Grant P42 ESO7373 (C.Y. Chen) from the National Institute of Environmental Health Sciences. An earlier version of the manuscript was reviewed by two anonymous referees. NR 61 TC 4 Z9 4 U1 1 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD AUG PY 2012 VL 32 IS 4 BP 653 EP 663 DI 10.1007/s13157-012-0298-4 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 976ON UT WOS:000306594300006 ER PT J AU Goebel, PC Pregitzer, KS Palik, BJ AF Goebel, P. Charles Pregitzer, Kurt S. Palik, Brian J. TI Influence of Flooding and Landform Properties on Riparian Plant Communities in an Old-Growth Northern Hardwood Watershed SO WETLANDS LA English DT Article DE Flooding; Ecotones; Fluvial landforms; Hydrologic modeling; Northern hardwood forest ecosystems; Great Lakes ID DISSOLVED ORGANIC-CARBON; LOWER MICHIGAN; PASSAGE-CREEK; TANANA RIVER; LARGE-WOOD; VEGETATION; FOREST; FLOODPLAIN; USA; DISTURBANCE AB In most forested landscapes, the organization of plant communities across stream valleys is thought to be regulated by a complex set of interactions including flooding, landform properties, and vegetation. However, few studies have directly examined the relative influence of frequent and infrequent flooding, as well as landform properties, on riparian plant community organization in moderately or deeply entrenched stream valleys where the magnitude and extent of frequent flooding may be constrained by local stream valley characteristics. Our approach, which we applied in an old-growth northern hardwood watershed, integrated detailed plant community surveys with a GIS and watershed surface hydrology model that allowed us to model water surface elevation associated with different flood magnitudes and recurrence intervals for specific locations across the old-growth watershed. Our results show that irrespective of stream valley geomorphology, the ground-flora exhibits a high rate of species replacement across the stream valley at low elevations, which are the most susceptible to frequent and more extreme infrequent flooding. However, over 50 % of the major shifts in ground-flora community composition and almost all of the shifts in overstory composition occur beyond the direct influence of flooding, especially in the high-gradient moderately and deeply entrenched stream valleys. In these areas, landform boundaries and changes in the environmental properties associated with these boundaries appear to be the primary factors controlling changes in vegetation across the stream valley. C1 [Goebel, P. Charles] Ohio State Univ, Ohio Agr Res & Dev Ctr, Sch Environm & Nat Resources, Wooster, OH 44691 USA. [Pregitzer, Kurt S.] Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA. [Palik, Brian J.] US Forest Serv, No Res Stn, USDA, Grand Rapids, MN 55744 USA. RP Goebel, PC (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Sch Environm & Nat Resources, 1680 Madison Ave, Wooster, OH 44691 USA. EM goebel.11@osu.edu RI Goebel, Patrick/B-3657-2012 FU USDA Forest Service, North Central Research Station, School of Forestry and Wood Products, Michigan Technological University; Ohio Agricultural Research and Development Center at The Ohio State University FX The USDA Forest Service, North Central Research Station, School of Forestry and Wood Products, Michigan Technological University, and Ohio Agricultural Research and Development Center at The Ohio State University provided support for this study. We thank Anita Risch, Anitra Bennett, and Michelle Jarvi for assistance with data collection. Additionally, we appreciate the support of Jiquan Chen, Peg Gale, Robert Naiman, and two anonymous referees who reviewed earlier versions of this manuscript. NR 58 TC 3 Z9 6 U1 5 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD AUG PY 2012 VL 32 IS 4 BP 679 EP 691 DI 10.1007/s13157-012-0300-1 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 976ON UT WOS:000306594300008 ER PT J AU Coyle, DR Mattson, WJ Jordan, MS Raffa, KF AF Coyle, David R. Mattson, William J., Jr. Jordan, Michelle S. Raffa, Kenneth F. TI Variable host phenology does not pose a barrier to invasive weevils in a northern hardwood forest SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Barypeithes pellucidus; belowground herbivory; Coleoptera; Curculionidae; exotic species; Phyllobius oblongus; Polydrusus sericeus; Sciaphilus asperatus ID PHYLLOBIUS-OBLONGUS COLEOPTERA; GREAT-LAKES REGION; HERBIVOROUS INSECTS; POLYDRUSUS-SERICEUS; BUDBURST PHENOLOGY; LARVAL DEVELOPMENT; PLANT-QUALITY; LIFE-HISTORY; PERFORMANCE; CURCULIONIDAE AB 1 A suite of invasive weevils has established in hardwood forests of the North American Great Lakes Region. We quantified patterns of host availability and the capacity of adults to succeed in a system with high host variability both within and between seasons in Michigan, U.S.A. 2 We quantified phenological development of foliage on three host species [sugar maple, Acer saccharum Marshall; ironwood, Ostrya virginiana (Mill.) K. Koch; and raspberry, Rubus spp.]. We estimated adult abundance using emergence traps and sweep net sampling over 3 years, and compared field host associations with laboratory choice assays. 3 Host plant phenology varied among species, between years, and in their interactions. The four most common weevils, Phyllobius oblongus (L.), Polydrusus sericeus (Schaller), Barypeithes pellucidus (Boheman) and Sciaphilus asperatus (Bonsdorff), emerged in early to mid-June, in approximately that order. After emergence, each species showed evidence of host preference based on their abundances on foliage. Overall, P. oblongus and B. pellucidus were most prevalent on sugar maple, P. sericeus was most prevalent on ironwood, and S. asperatus was relatively evenly distributed. Laboratory choice tests with P. oblongus and P. sericeus confirmed these preferences. 4 These four invasive species comprised over 99% of all 12 845 weevils obtained, suggesting displacement of native species. The optimal sampling methods varied among weevil species. 5 These invasive weevils contend with the highly variable conditions of their environment, and also potential phenological asynchrony, via relatively late emergence, even at the cost of lower host quality. Annual variation is greater for numbers of adults than larvae, suggesting that mortality of late instars or pupae is particularly important. C1 [Coyle, David R.; Jordan, Michelle S.; Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Mattson, William J., Jr.] US Forest Serv, USDA, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. RP Coyle, DR (reprint author), Univ Georgia, DB Warnell Sch Forestry & Nat Resources, 180 Green St, Athens, GA 30602 USA. EM dcoyle@warnell.uga.edu FU UW College of Agricultural and Life Sciences, Applied Ecological Services, Inc. (Brodhead, Wisconsin) [WIS04969]; United States Environmental Protection Agency (EPA) FX We thank K. Buechel, A. Czechowicz, M. Jones, C. Lapin, M. Komp and J. Schmidt (UW-Madison) for their assistance with field sampling and bioassays. We thank P. Crump and R. Tang (UW-Madison) for statistical consultation. We appreciate the thoughtful discussion and reviews by C. Gratton, D. Hogg and T. Balser (UW-Madison). We thank the Ball Corp. (Watertown, Wisconsin) for graciously donating materials for emergence traps. This research has been funded wholly or in part by McIntire-Stennis Project No. WIS04969, UW College of Agricultural and Life Sciences, Applied Ecological Services, Inc. (Brodhead, Wisconsin), and the United States Environmental Protection Agency (EPA) under the Science to Achieve Results (STAR) Graduate Fellowship Program. EPA has not officially endorsed this publication and the views expressed herein may not reflect those of the EPA. This article reports the results of research only. Mention of a commercial or proprietary product does not constitute an endorsement or a recommendation by the USDA or EPA for its use. NR 46 TC 4 Z9 4 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2012 VL 14 IS 3 BP 276 EP 285 DI 10.1111/j.1461-9563.2012.00567.x PG 10 WC Entomology SC Entomology GA 972UT UT WOS:000306307100006 ER PT J AU Chen, YG Ulyshen, MD Poland, TM AF Chen, Yigen Ulyshen, Michael D. Poland, Therese M. TI Differential utilization of ash phloem by emerald ash borer larvae: ash species and larval stage effects SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Agrilus planipennis; amino acids; Fraxinus; nutritional ecology; phenolics; plant-insect interactions ID SPODOPTERA-EXIGUA; AMINO-ACIDS; BOMBYX-MORI; INSECTS; PREFERENCE; CHEMISTRY; SUITABILITY; PERFORMANCE; HERBIVORES; QUALITY AB 1 Two experiments were performed to determine the extent to which ash species (black, green and white) and larval developmental stage (second, third and fourth instar) affect the efficiency of phloem amino acid utilization by emerald ash borer (EAB) Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) larvae. 2 EAB larvae generally utilized green ash amino acids more efficiently than those of the other two species. For example, the concentrations of only six (two essential) and seven (two essential) amino acids were lower in frass from EAB that fed upon black and white ash than in the corresponding phloem, respectively. By contrast, concentrations of 16 (eight essential) amino acids were lower in the frass from EAB that fed upon green ash than in the phloem. In addition, in green ash, the frass : phloem ratios of 13 amino acids were lower than their counterparts in black and white ash. 3 The concentrations of non-essential amino acids glycine and hydroxylproline were greater in frass than in phloem when EAB fed on black ash, although not when EAB fed on green or white ash. 4 The concentration of total phenolics (a group of putative defensive compounds to EAB, expressed as antioxidant activity of acetone extraction) was high in EAB frass but even higher in the phloem samples when the data were pooled across ash species and EAB larval stages. This suggests EAB larvae may eliminate phenolics through a combination of direct excretion and enzymatic conversion of phenolics to nonphenolics before excretion. Because the ratio of frass total phenolics to phloem total phenolics in white ash was lower than the ratios in black and green ash, the ability to destroy phenolics or convert them to nonphenolics was greater when EAB larvae fed on white ash. 5 Fourth-instar EAB extracted phloem amino acids, including threonine, more efficiently than third-instar EAB. The different larval developmental stages of EAB did not differ in their apparent ability to destroy phenolics or convert them to nonphenolics. C1 [Chen, Yigen; Ulyshen, Michael D.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Ulyshen, Michael D.] US Forest Serv, USDA, So Res Stn, Starkville, MS 39759 USA. [Poland, Therese M.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. RP Chen, YG (reprint author), Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. EM ygchen2007@gmail.com FU USDA Forest Service, NA FHP FX We thank Drs Deguang Liu (University of California, Davis), Vanessa Muilenburg (Ohio State University) and six anonymous reviewers for providing insightful comments that significantly improved an early draft of the manuscript. The research was supported by the USDA Forest Service, NA FHP. NR 37 TC 4 Z9 4 U1 2 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2012 VL 14 IS 3 BP 324 EP 330 DI 10.1111/j.1461-9563.2012.00572.x PG 7 WC Entomology SC Entomology GA 972UT UT WOS:000306307100011 ER PT J AU Swope, SM Stein, IR AF Swope, Sarah M. Stein, Ilana R. TI Soil type mediates indirect interactions between Centaurea solstitialis and its biocontrol agents SO BIOLOGICAL INVASIONS LA English DT Article DE Calcium; Chaetorellia; Eustenopus villosus; Nitrogen; Puccinia jaceae solstitialis; Serpentine; Systemic acquired resistance ID PLANT-PATHOGEN INTERACTIONS; SIGNAL-TRANSDUCTION; YELLOW STARTHISTLE; SERPENTINE FLAX; RESISTANCE; FUNGUS; HERBIVORES; INSECT; RUST; COMPETITION AB Many invasive plants are attacked by more than one biocontrol agent. Attack by multiple enemies may give rise to indirect interactions, the nature of which may be influenced by the abiotic environment. We conducted a field experiment to determine (1) whether indirect interactions arose between Centaurea solstitialis, a foliar pathogen and three insect seed predators and (2) how the outcome was influenced by soil type (serpentine and non-serpentine). Because serpentine soils support high numbers of endemic species they are a priority for conservation. They also have very low calcium concentrations and Ca++ regulates plants' ability to defend against pathogen infection. C. solstitialis growing on serpentine soil may therefore be more vulnerable to the pathogen and this may in turn affect the plant's subsequent interactions with seed predators. We found that pathogen infection had a direct, negative impact on plant performance but its impact was not greater on serpentine plants. When attacked by the seed predators, inflorescences produced more viable seed when they were on plants infected with the pathogen than when they were on uninfected plants and the data suggest that this reflects reductions in larval seed-feeding. On the non-serpentine soil, the pathogen's direct, negative impact was entirely canceled out by its indirect, positive effect via reduced seed predation. On the serpentine soil, plants attacked by the pathogen and the insect seed predators produced half as many seeds than plants attacked only by the seed predators. Our results demonstrate that biocontrol agent interactions may be modified by the plant and by the abiotic environment in a way that fundamentally alters their net impact on the weed. C1 [Swope, Sarah M.] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Stein, Ilana R.] Mills Coll, Dept Biol, Oakland, CA 94613 USA. RP Swope, SM (reprint author), USDA, Great Basin Rangeland Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM sarah.swope@ars.usda.gov FU Jill Barrett Biology Research Program, Mills College; USDA ARS Exotic and Invasive Weeds Research Unit FX Special thanks to John Harris (Mills College, Oakland, CA). IRS was supported by Jill Barrett Biology Research Program, Mills College. We thank Ingrid Parker, Andrea Jani and two anonymous reviewers for comments that improved an earlier version of the manuscript, as well as Joe Braasch, Matt Jones, Sabrina McCue, Sandra Li and Tye Morgan for their work in the field and lab. We thank Dale Woods (CDFA) for providing Puccinia spores and Paul Aigner and Cathy Koehler for their logistical support at McLaughlin Natural Reserve. SMS is grateful for the support of the USDA ARS Exotic and Invasive Weeds Research Unit. The USDA is an equal opportunity employer. NR 53 TC 5 Z9 5 U1 2 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD AUG PY 2012 VL 14 IS 8 BP 1697 EP 1710 DI 10.1007/s10530-012-0181-5 PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 973DL UT WOS:000306334500016 ER PT J AU Harris, CC Nielsen, EA Becker, DR Blahna, DJ McLaughlin, WJ AF Harris, Charles C. Nielsen, Erik A. Becker, Dennis R. Blahna, Dale J. McLaughlin, William J. TI Results of Community Deliberation About Social Impacts of Ecological Restoration: Comparing Public Input of Self-Selected Versus Actively Engaged Community Members SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Social impact assessment; Public involvement; Public deliberation; Community-based resource management; Ecological restoration; Salmon recovery ID NATURAL-RESOURCE MANAGEMENT; CITIZEN PARTICIPATION; DECISION-MAKING; ATTITUDES; MECHANISMS; PERSPECTIVES; INVOLVEMENT; KNOWLEDGE; POLICY AB Participatory processes for obtaining residents' input about community impacts of proposed environmental management actions have long raised concerns about who participates in public involvement efforts and whose interests they represent. This study explored methods of broad-based involvement and the role of deliberation in social impact assessment. Interactive community forums were conducted in 27 communities to solicit public input on proposed alternatives for recovering wild salmon in the Pacific Northwest US. Individuals identified by fellow residents as most active and involved in community affairs ("AE residents") were invited to participate in deliberations about likely social impacts of proposed engineering and ecological actions such as dam removal. Judgments of these AE participants about community impacts were compared with the judgments of residents motivated to attend a forum out of personal interest, who were designated as self-selected ("SS") participants. While the magnitude of impacts rated by SS participants across all communities differed significantly from AE participants' ratings, in-depth analysis of results from two community case studies found that both AE and SS participants identified a large and diverse set of unique impacts, as well as many of the same kinds of impacts. Thus, inclusion of both kinds of residents resulted in a greater range of impacts for consideration in the environmental impact study. The case study results also found that the extent to which similar kinds of impacts are specified by AE and SS group members can differ by type of community. Study results caution against simplistic conclusions drawn from this approach to community-wide public participation. Nonetheless, the results affirm that deliberative methods for community-based impact assessment involving both AE and SS residents can provide a more complete picture of perceived impacts of proposed restoration activities. C1 [Harris, Charles C.] Univ Idaho, Dept Conservat Social Sci, Moscow, ID 83844 USA. [Nielsen, Erik A.] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. [Becker, Dennis R.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Blahna, Dale J.] US Forest Serv, Pacific NW Res Stn, USDA, Seattle, WA USA. [McLaughlin, William J.] Univ Idaho, Coll Nat Resources, Moscow, ID 83843 USA. RP Harris, CC (reprint author), Univ Idaho, Dept Conservat Social Sci, Moscow, ID 83844 USA. EM charris@uidaho.edu; erik.nielsen@nau.edu; drbecker@umn.edu; dblahna@fs.fed.us; billm@uidaho.edu RI Becker, Dennis/E-6249-2017 FU U.S. Army Corps of Engineers, Walla Walla District, Walla Walla, WA FX An early version of this article was drafted and data collected with funding provided by the U.S. Army Corps of Engineers, Walla Walla District, Walla Walla, WA; support for further analysis of those data and development of the current article was provided by the Pacific Northwest Research Station, Seattle, WA. NR 59 TC 2 Z9 3 U1 1 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X J9 ENVIRON MANAGE JI Environ. Manage. PD AUG PY 2012 VL 50 IS 2 BP 191 EP 203 DI 10.1007/s00267-012-9871-0 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA 971AO UT WOS:000306174200002 PM 22615108 ER PT J AU Meurs, KM Lahmers, S Keene, BW White, SN Oyama, MA Mauceli, E Lindblad-Toh, K AF Meurs, Kathryn M. Lahmers, Sunshine Keene, Bruce W. White, Stephen N. Oyama, Mark A. Mauceli, Evan Lindblad-Toh, Kerstin TI A splice site mutation in a gene encoding for PDK4, a mitochondrial protein, is associated with the development of dilated cardiomyopathy in the Doberman pinscher SO HUMAN GENETICS LA English DT Article ID CANDIDATE GENE; DOGS; HEART; METABOLISM; OXIDATION; DNA AB Familial dilated cardiomyopathy is a primary myocardial disease that can result in the development of congestive heart failure and sudden cardiac death. Spontaneous animal models of familial dilated cardiomyopathy exist and the Doberman pinscher dog is one of the most commonly reported canine breeds. The objective of this study was to evaluate familial dilated cardiomyopathy in the Doberman pinscher dog using a genome-wide association study for a genetic alteration(s) associated with the development of this disease in this canine model. Genome-wide association analysis identified an area of statistical significance on canine chromosome 14 (p (raw) = 9.999e-05 corrected for genome-wide significance), fine-mapping of additional SNPs flanking this region localized a signal to 23,774,190-23,781,919 (p = 0.001) and DNA sequencing identified a 16-base pair deletion in the 5' donor splice site of intron 10 of the pyruvate dehydrogenase kinase 4 gene in affected dogs (p < 0.0001). Electron microscopy of myocardium from affected dogs demonstrated disorganization of the Z line, mild to moderate T tubule and sarcoplasmic reticulum dilation, marked pleomorphic mitochondrial alterations with megamitochondria, scattered mitochondria with whorling and vacuolization and mild aggregates of lipofuscin granules. In conclusion, we report the identification of a splice site deletion in the PDK4 gene that is associated with the development of familial dilated cardiomyopathy in the Doberman pinscher dog. C1 [Meurs, Kathryn M.; Keene, Bruce W.] N Carolina State Univ, Coll Vet Med, Raleigh, NC 27695 USA. [Meurs, Kathryn M.; Lahmers, Sunshine; White, Stephen N.] Washington State Univ, Coll Vet Med, Pullman, WA 99164 USA. [White, Stephen N.] ARS, USDA, Anim Dis Res Unit, Pullman, WA USA. [Oyama, Mark A.] Univ Penn, Sch Vet Med, Philadelphia, PA 19104 USA. [Mauceli, Evan; Lindblad-Toh, Kerstin] Broad Inst MIT & Harvard, Cambridge, MA USA. [Lindblad-Toh, Kerstin] Uppsala Univ, Uppsala, Sweden. RP Meurs, KM (reprint author), N Carolina State Univ, Coll Vet Med, Raleigh, NC 27695 USA. EM kate_meurs@ncsu.edu OI White, Stephen N./0000-0001-9255-6054 NR 32 TC 26 Z9 26 U1 1 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0340-6717 J9 HUM GENET JI Hum. Genet. PD AUG PY 2012 VL 131 IS 8 BP 1319 EP 1325 DI 10.1007/s00439-012-1158-2 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA 974KI UT WOS:000306433400004 PM 22447147 ER PT J AU Burdick, NC Bernhard, BC Carroll, JA Rathmann, RJ Johnson, BJ AF Burdick, Nicole C. Bernhard, Bryan C. Carroll, Jeffery A. Rathmann, Ryan J. Johnson, Bradley J. TI Enhancement of the acute phase response to a lipopolysaccharide challenge in steers supplemented with chromium SO INNATE IMMUNITY LA English DT Article DE Acute phase response; chromium; lipopolysaccharide; sickness behavior; temperature ID STRESSED FEEDER CALVES; BOVINE RESPIRATORY-DISEASE; LACTATION DAIRY-COWS; RECTAL TEMPERATURE; IMMUNE-RESPONSES; PERFORMANCE; CATTLE; EXCRETION; VACCINES; HEALTH AB The study examined the effect of chromium supplementation on the response of steers to an LPS challenge. Steers received a premix that added 0 (control; n = 10) or 0.2 mg/kg of chromium (n = 10) to the total diet on a dry matter basis for 56 d. Steers were fitted with jugular catheters and rectal temperature (RT) recording devices on d 52. Blood samples were collected and sickness behavior scores assigned to each steer relative to an LPS challenge (0.5 mu g/kg) on d 55. Pre-LPS RT were greater in chromium-supplemented than in control steers. Post-LPS RT increased in both treatments, with control steers producing a greater change in RT than chromium-supplemented steers. Sickness behavior scores were greater in control than in chromium-supplemented steers post-LPS (P = 0.03). Cortisol concentrations did not differ between treatments pre-LPS. Post-LPS cortisol concentrations increased but did not differ due to treatment. Concentrations of IL-4 increased post-LPS but were not affected by treatment pre- or post-LPS. Treatment did not affect pre-LPS TNF-alpha or IFN-gamma. Post-LPS TNF-alpha and IFN-gamma increased in both treatments, with chromium-supplemented steers producing greater TNF-alpha (P = 0.005) and IFN-gamma (P = 0.004) than control steers. Pre-LPS IL-6 was greater (P = 0.027) in chromium-supplemented steers than in control steers. Post-LPS IL-6 increased in both treatments and was greater (P < 0.001) in chromium-supplemented than in control steers. These data suggest that chromium supplementation enhances the acute phase response of steers to an LPS challenge, which may expedite recovery. C1 [Burdick, Nicole C.; Carroll, Jeffery A.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Bernhard, Bryan C.; Rathmann, Ryan J.; Johnson, Bradley J.] Texas Tech Univ, Dept & Anim & Food Sci, Lubbock, TX 79409 USA. RP Carroll, JA (reprint author), ARS, USDA, Livestock Issues Res Unit, 1604 E FM 1294, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov FU Kemin Agrifoods, North America, Inc., Des Moines, IA, USA; Gordon W. Davis Regents Chair Endowment at Texas Tech University FX This study was supported in part by Kemin Agrifoods, North America, Inc., Des Moines, IA, USA, and the Gordon W. Davis Regents Chair Endowment at Texas Tech University. NR 33 TC 4 Z9 4 U1 1 U2 6 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1753-4259 J9 INNATE IMMUN-LONDON JI Innate Immun. PD AUG PY 2012 VL 18 IS 4 BP 592 EP 601 DI 10.1177/1753425911428964 PG 10 WC Biochemistry & Molecular Biology; Immunology; Medicine, Research & Experimental; Microbiology SC Biochemistry & Molecular Biology; Immunology; Research & Experimental Medicine; Microbiology GA 974HQ UT WOS:000306424200004 PM 22180563 ER PT J AU Mitchell-Blackwood, J Gurian, PL Lee, R Thran, B AF Mitchell-Blackwood, J. Gurian, P. L. Lee, R. Thran, B. TI Variance in Bacillus anthracis virulence assessed through Bayesian hierarchical dose-response modelling SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Bacillus anthracis; dose-response modelling; environmental; spores; virulence ID ENVIRONMENTAL EXPOSURE; RISK; INHALATION; SPORES AB Aims: To develop a predictive doseresponse model for describing the survival of animals exposed to Bacillus anthracis to support risk management options. Methods and Results: Doseresponse curves were generated from a large dosemortality data set (>11 000 data points) consisting of guinea pigs exposed via the inhalation route to 76 different product preparations of B.similar to anthracis. Because of the predictive nature of the Bayesian hierarchical approach (BHA), this method was used. The utility of this method in planning for a variety of scenarios from best case to worst case was demonstrated. Conclusions: A wide range of expected virulence was observed across products. Median estimates of virulence match well with previously published statistical estimates, but upper bound values of virulence are much greater than previous statistical estimates. Significance and Impact of the Study: This study is the first meta-analysis in open literature to estimate the doseresponse relationship for B.similar to anthracis from a very large data set, generally a rare occurrence for highly infectious pathogens. The results are also the first to suggest the extent of variability, which is contributed by product preparation and/or dissemination methods, information needed for health-based risk management decisions in response to a deliberate release. A set of possible benchmark values produced through this analysis can be tied to the risk tolerance of the decision-maker or available intelligence. Further, the substantial size of the data set led to the ability to assess the appropriateness of the assumed distributional form of the prior, a common limitation in Bayesian analysis. C1 [Mitchell-Blackwood, J.; Gurian, P. L.] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA. [Mitchell-Blackwood, J.] US EPA, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA. [Lee, R.] USA, Med Res Inst Chem Def, Aberdeen Proving Ground, MD 21010 USA. [Lee, R.; Thran, B.] USA, Environm Hlth Risk Assessment Program, Hlth Risk Management Portfolio, Publ Hlth Command Army Inst Publ Hlth, Aberdeen Proving Ground, MD USA. RP Mitchell-Blackwood, J (reprint author), Food Safety & Inspect Serv, USDA, 1400 Independence Ave,Stop 3766, Washington, DC 20250 USA. EM mitchell-blackwood.jade@epa.gov RI Gurian, Patrick/A-8365-2013 OI Gurian, Patrick/0000-0001-7456-9740 FU US Army, of the Pathogen Information Catalog (PICAT); US Army Center for Health Promotion and Preventive Medicine (USACHPPM); Center for Advancing Microbial Risk Assessment; US Department of Homeland Security; US Environmental Protection Agency under the Science to Achieve Results (STAR) grant program [R83236201] FX The authors would like to thank Drs Tonya Nichols and Sarah Taft (US Environmental Protection Agency, National Homeland Security Research Center) for joint financial support, with the US Army, of the Pathogen Information Catalog (PICAT), which houses the data set, and their technical contributions to the project. Stephanie Hines (Battelle) also contributed during technical discussions. Ms. Erin Curran (US Army Public Health Command was instrumental in the gathering of the original research reports, and Peg Coleman, Stacey Massulik, Michele Stephenson, and Lisa Lowe (Syracuse Research Corporation) were instrumental in the population of the PICAT. Portions of this research were supported in part by an appointment to the Student Research Participation Program at the US Army Center for Health Promotion and Preventive Medicine (USACHPPM) administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the US Department of Energy and USACHPPM. Additional support was provided by the Center for Advancing Microbial Risk Assessment, which is supported by the US Department of Homeland Security and the US Environmental Protection Agency under the Science to Achieve Results (STAR) grant program (grant no. R83236201). NR 18 TC 4 Z9 4 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD AUG PY 2012 VL 113 IS 2 BP 265 EP 275 DI 10.1111/j.1365-2672.2012.05311.x PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 974AF UT WOS:000306401300005 PM 22515543 ER PT J AU Herald, TJ Gadgil, P Tilley, A AF Herald, Thomas J. Gadgil, Priyadarshini Tilley, Andmichael TI High-throughput micro plate assays for screening flavonoid content and DPPH-scavenging activity in sorghum bran and flour SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE 96-well plate; sorghum; phenolic compound; flavonoid; DPPH; antioxidant ID ANTIOXIDANT ACTIVITY; PHENOLIC CONTENT; MILLED FRACTIONS; WHEAT; PHYTOCHEMICALS; VARIETIES; CAPACITY AB BACKGROUND: Sorghum possesses phenolic compounds that are health-promoting constituents of the grain. There are approximately 40 000 sorghum accessions, many of which have not been evaluated for the grain's health-promoting potential. Conventional methods for measuring total phenolic content, flavonoid content and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging capacity are time-consuming and labour-intensive, resulting in low overall throughput. The objective of this study was to develop a high-throughput screening assay for large sorghum sample sets to determine flavonoid and phenolic content and to modify existing DPPH and total phenolic assays. RESULTS: The 96-well assays exhibited a correlation of > 0.9 with the conventional assays. The 96-well assays allowed for up to 64 samples to be run per day compared with 20-24 samples (depending on the test) for the conventional methods. The 96-well assays had excellent accuracy (97.65-106.16% recovery), precision (1.06-8.28% coefficient of variation (CV)) and reproducibility (1.32-2.13% CV inter-day and 1.36-2.09% CV intra-day). CONCLUSION: The high-throughput 96-well plate method proved to be as robust and reproducible as the conventional method for determining total phenolic content, flavonoid content and DPPH-scavenging capacity in either sorghum bran or flour. Published 2012 by John Wiley & Sons, Ltd. C1 [Herald, Thomas J.; Gadgil, Priyadarshini; Tilley, Andmichael] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Herald, TJ (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM tom.herald@ars.usda.gov NR 23 TC 28 Z9 31 U1 2 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD AUG PY 2012 VL 92 IS 11 BP 2326 EP 2331 DI 10.1002/jsfa.5633 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 973ZX UT WOS:000306400500018 PM 22419130 ER PT J AU Avena-Bustillos, RJ Du, WX Woods, R Olson, D Breksa, AP McHugh, TH AF Avena-Bustillos, Roberto J. Du, Wen-Xian Woods, Rachelle Olson, Donald Breksa, Andrew P., III McHugh, Tara H. TI Ultraviolet-B light treatment increases antioxidant capacity of carrot products SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE carrots (Daucus carota); UV-B; fresh-cut; total soluble phenolics ID PHENYLALANINE AMMONIA-LYASE; PHENOLIC CONTENT; POSTHARVEST DISEASES; DIETARY FLAVONOIDS; DAUCUS-CAROTA; L.; FRUIT; LETTUCE; BLUEBERRIES; IRRADIATION AB BACKGROUND: The effect of ultraviolet-B (UV-B) light as a postharvest treatment to enhance the antioxidant content of carrots and fresh-cut carrot products was evaluated. Four levels of UV-B dose ranging from 1.3 to 12 kJ m-2 were applied to whole, baby and various styles of cut carrots, and the changes in antioxidant capacity, total soluble phenolics and phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) activity were measured after a 3 day incubation period at 15 degrees C and 45% relative humidity. RESULTS: Both cutting style and dose level were factors in determining carrot responses to UV-B treatment. Antioxidant capacity increased significantly (1.46.6-fold). Total soluble phenolic results correlated directly with those of antioxidant capacity (R2 = 0.953), indicating that the enhancements achieved were due to an increase in phenolic content. High-performance liquid chromatography analysis revealed that 5-O-caffeoylquinic acid (5-CQA) was the primary phenolic responsible for this increase. Higher PAL activity was also observed in UV-B-treated samples, indicating that the increase in 5-CQA was a biological response to UV-B exposure. CONCLUSION: UV-B treatment has the potential to increase the nutritional value of carrots and offers an exciting opportunity to increase consumer accessibility to dietary choices that are rich in antioxidants. Published 2012 by John Wiley & Sons, Ltd. C1 [Du, Wen-Xian; Woods, Rachelle; Olson, Donald; Breksa, Andrew P., III; McHugh, Tara H.] USDA ARS, Proc Foods Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Avena-Bustillos, Roberto J.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP Du, WX (reprint author), USDA ARS, Proc Foods Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM wen-xian.du@ars.usda.gov FU USDA National Institute of Food and Agriculture [2009-65503-05811] FX This project was supported by Agriculture and Food Research Initiative Grant 2009-65503-05811 from the USDA National Institute of Food and Agriculture. Grimmway Farms kindly provided the different carrot products used in this study. Thanks to Brian Cain for assistance with the HPLC analysis. NR 42 TC 12 Z9 13 U1 0 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD AUG PY 2012 VL 92 IS 11 BP 2341 EP 2348 DI 10.1002/jsfa.5635 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 973ZX UT WOS:000306400500020 PM 22419219 ER PT J AU Brown, CR Vales, I Yilma, S James, S Charlton, B Culp, D Hane, D Shock, C Feibert, E Pavek, M Knowles, R Novy, R Whitworth, J Stark, J Miller, JC Holm, D Quick, R Navarre, R AF Brown, Charles R. Vales, Isabel Yilma, Solomon James, Steven Charlton, Brian Culp, Darrin Hane, Dan Shock, Clinton Feibert, Eric Pavek, Mark Knowles, Richard Novy, Richard Whitworth, Jonathan Stark, Jeff Miller, J. Creighton, Jr. Holm, David Quick, Richard Navarre, Roy TI "AmaRosa," a Red Skinned, Red Fleshed Fingerling with High Phytonutrient Value SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Antioxidants; Fingerling; Anthocyanin; ORAC ID ANTIOXIDANT ACTIVITY; SOLANUM-TUBEROSUM; POTATO CULTIVARS; PHENOLIC CONTENT; VARIABILITY; ANTHOCYANIN AB The diversity of traits in varieties of potato outside of its South American birthplace is a small subset of that available in the Andean center of origin. Among the traits that evoke most interest are skin and flesh pigments. Recent studies have pointed to the high antioxidant activity and potential healthful benefits from these pigments or other antioxidant compounds. The market for potatoes with unusual color patterns has been supplied largely by heirloom varieties of uncertain origin and the highly successful Yukon Gold. Interest has intensified and been transformed into a focused effort in the breeding of specialty varieties, remarkable for their unusual colors. The purpose of this paper is to describe a new potato variety in the Fingerling Class with red skin and red flesh. C1 [Brown, Charles R.; Quick, Richard; Navarre, Roy] ARS, USDA, Prosser, WA 99350 USA. [Vales, Isabel; Yilma, Solomon] Oregon State Univ, Dept Crop Sci, Corvallis, OR 97333 USA. [James, Steven; Charlton, Brian; Culp, Darrin] Oregon State Univ, KBREC, Klamath Falls, OR 97601 USA. [James, Steven] Oregon State Univ, COES, Madras, OR 97741 USA. [Hane, Dan] Oregon State Univ, HAREC, Hermiston, OR 97838 USA. [Shock, Clinton; Feibert, Eric] Oregon State Univ, MES, Ontario, OR 97914 USA. [Pavek, Mark; Knowles, Richard] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Stark, Jeff] Univ Idaho, AREC, Aberdeen, ID 83210 USA. [Novy, Richard; Whitworth, Jonathan] ARS, USDA, Aberdeen, ID 83210 USA. [Miller, J. Creighton, Jr.] Texas A&M Univ, Dept Hort, College Stn, TX 77843 USA. [Holm, David] Colorado State Univ, SLVRC, Dept Hort & Landscape Architecture, Ft Collins, CO 81125 USA. RP Brown, CR (reprint author), ARS, USDA, Prosser, WA 99350 USA. EM chuck.brown@ars.usda.gov NR 10 TC 3 Z9 3 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2012 VL 89 IS 4 BP 249 EP 254 DI 10.1007/s12230-012-9248-1 PG 6 WC Agronomy SC Agriculture GA 973CF UT WOS:000306329600001 ER PT J AU Brown, CR Haynes, KG Moore, M Pavek, MJ Hane, DC Love, SL Novy, RG Miller, JC AF Brown, C. R. Haynes, K. G. Moore, M. Pavek, M. J. Hane, D. C. Love, S. L. Novy, R. G. Miller, J. C., Jr. TI Stability and Broad-Sense Heritability of Mineral Content in Potato: Calcium and Magnesium SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Genotype x environment interaction; Breeding; ICAEPS; Biofortification ID TUBER CALCIUM; PLANTS; GERMPLASM; CULTIVARS; NUTRITION; NECROSIS; STRESS AB Calcium and magnesium are two minerals that play prominent roles in animal and plant metabolism. The purpose of this study was to determine if genetic variation exists among advanced potato breeding clones for tuber calcium and magnesium content and the extent of genotype x environment interactions on these two traits. Ten, 13, and 13 clones were evaluated in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Tuber calcium content ranged from 266 to 944 mu g-g(-1) DW; magnesium from 787 to 1,089 mu g-g(-1) DW. Genotype x environment interactions were significant in all trials. However, only the Tri-State for calcium and the Western Regional Red/Specialty trials for both minerals displayed a significant source of variation for genotypes. Broad-sense heritabilities for tuber calcium content were 0.65, 0.37 and 0 in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Broad-sense heritabilities for tuber magnesium content were 0.57, 0, and 0.72 in the Tri-State, Western Regional, and Western Regional Red/Specialty Trials, respectively. Potato is not a rich source of either calcium or magnesium for the human diet, but genetic variation exists among potato clones that might be useful for plant health. C1 [Brown, C. R.] ARS, USDA, Prosser, WA 99350 USA. [Haynes, K. G.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Moore, M.] Agri NW, Plymouth, WA 99346 USA. [Pavek, M. J.] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Hane, D. C.] Oregon State Univ, HAREC, Dept Crop Sci, Hermiston, OR 97838 USA. [Love, S. L.] Univ Idaho, AREC, Aberdeen, ID 83210 USA. [Novy, R. G.] ARS, USDA, Aberdeen, ID 83210 USA. [Miller, J. C., Jr.] Texas A&M Univ, Dept Hort, College Stn, TX 77843 USA. RP Brown, CR (reprint author), ARS, USDA, Prosser, WA 99350 USA. EM chuck.brown@ars.usda.gov NR 26 TC 5 Z9 5 U1 2 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2012 VL 89 IS 4 BP 255 EP 261 DI 10.1007/s12230-012-9240-9 PG 7 WC Agronomy SC Agriculture GA 973CF UT WOS:000306329600002 ER PT J AU McCord, P Zhang, LH Brown, C AF McCord, Per Zhang, Linhai Brown, Chuck TI The Incidence and Effect on Total Tuber Carotenoids of a Recessive Zeaxanthin Epoxidase Allele (Zep1) in Yellow-fleshed Potatoes SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Carotenoids; Zeaxanthin epoxidase; Potato breeding; Marker-assisted breeding ID ANTIOXIDANT VALUES; ANTHOCYANIN AB Zeaxanthin epoxidase (Zep) is one of at least two genes important for the incidence and amount of carotenoids in yellow-fleshed potato. The recessive allele of the gene encoding zeaxanthin epoxidase (Zep1) has previously been shown to inhibit the conversion of the xanthophyll zeaxanthin to other, more polar carotenoids. We examined the effect of the dosage of Zep1 on total carotenoids in yellow-fleshed tetraploid potato germplasm. The dosage of heterozygous individuals was determined using high-resolution DNA melting. We also surveyed a wide range of germplasm available in the USDA-ARS potato breeding program in Washington for the presence and dosage of Zep1. Genotypes with zero, one, or two copies of Zep1 had statistically similar levels of total tuber carotenoids. Triplex individuals showed a small but significant increase in total carotenoids over the previous three classes. In turn, individuals that were homozygous for Zep1 had much higher levels of total carotenoids than all other dosage classes. This suggests that a slight dosage effect is present, but that the effect of Zep1 is indeed largely recessive. A significant amount of variation in total carotenoids was observed within all Zep1 dosage classes, reinforcing the evidence that additional loci are important for high total carotenoids in potato. Our survey of breeding germplasm supported earlier research that Zep1 is uncommon in tetraploid potato germplasm. The use of high-resolution DNA melting again allowed us to identify the dosage of Zep1 in heterozygous individuals. This is important for breeding high-carotenoid potatoes at the tetraploid level, since recovery of homozygous Zep1 individuals is very rare. Identification of agronomically desirable parents with two or more copies of Zep1 would allow for higher recovery of homozygous Zep1 progeny, permitting additional selection for other desirable traits. C1 [McCord, Per; Brown, Chuck] ARS, USDA, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Zhang, Linhai] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Brown, C (reprint author), ARS, USDA, Vegetable & Forage Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM Chuck.brown@ars.usda.gov OI Zhang, Linhai/0000-0003-1009-6870 NR 13 TC 4 Z9 4 U1 1 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2012 VL 89 IS 4 BP 262 EP 268 DI 10.1007/s12230-012-9250-7 PG 7 WC Agronomy SC Agriculture GA 973CF UT WOS:000306329600003 ER PT J AU Vales, MI Brown, CR Yilma, S Hane, DC James, SR Shock, CC Charlton, BA Karaagac, E Mosley, AR Culp, D Feibert, E Stark, JC Pavek, MJ Knowles, NR Novy, RG Whitworth, JL AF Vales, M. I. Brown, C. R. Yilma, S. Hane, D. C. James, S. R. Shock, C. C. Charlton, B. A. Karaagac, E. Mosley, A. R. Culp, D. Feibert, E. Stark, J. C. Pavek, M. J. Knowles, N. R. Novy, R. G. Whitworth, J. L. TI Purple Pelisse: A Specialty 'Fingerling' Potato with Purple Skin and Flesh and Medium Specific Gravity SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Breeding; Antioxidants; Gourmet; Chipping quality ID SOLANUM-TUBEROSUM L. AB Purple Pelisse is a specialty 'fingerling' potato with purple skin and dark purple flesh. It has medium maturity and sets a large number of smooth, small, fingerling-shaped tubers. The tubers have medium specific gravity and high levels of antioxidants. This potato variety is mainly intended for the fresh market but it can also be used for processing; chips made from Purple Pelisse tubers retain their bright purple color and have a pleasant taste. Purple Pelisse is moderately resistant to common scab and potato virus Y and susceptible to most of the other common potato diseases. Purple Pelisse resulted from a cross between NDOP5847-1 and bulked pollen of red-fleshed potatoes. It was first selected from seedling tubers planted and grown at Madras, Oregon in 2001 and was subsequently evaluated for six additional years in public and industry trials throughout the western U.S, including Western Regional Red/Specialty Trials in 2006 and 2007. Purple Pelisse was officially released as a variety in 2009 by the Pacific Northwest (Tri-State) Potato Variety Development Program. C1 [Vales, M. I.; Yilma, S.; Karaagac, E.; Mosley, A. R.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Brown, C. R.] USDA ARS, Prosser, WA 99350 USA. [Hane, D. C.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [James, S. R.] Oregon State Univ, Cent Oregon Agr Res Ctr, Madras, OR 97741 USA. [Shock, C. C.; Feibert, E.] Oregon State Univ, Malheur Expt Stn, Ontario, ON 97914, Canada. [Charlton, B. A.; Culp, D.] Oregon State Univ, Klamath Basin Res & Extens Ctr, Klamath Falls, OR 97603 USA. [Stark, J. C.] Univ Idaho, Idaho Falls Res & Extens Ctr, Idaho Falls, ID 83402 USA. [Pavek, M. J.; Knowles, N. R.] Washington State Univ, Pullman, WA 99164 USA. [Novy, R. G.; Whitworth, J. L.] USDA ARS, Aberdeen Res & Extens Ctr, Aberdeen, ID 83210 USA. RP Vales, MI (reprint author), Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. EM i.vales@cgiar.org FU Oregon Agricultural Experiment Station; Oregon Potato Commission; Potato Variety Management Institute (PVMI); USDA Cooperative State Research, Education, and Extension Service; USDA Agricultural Research Service FX The authors thank Creighton Miller, David Holm, Roy Navarre, Jeanne Debons, Jenny Reed, Robert Smith, Lori Ewing, Margaret Bain, Nora Fuller, Zach Holden, Penny Tubbs, Darren Hall, Steve Wheeler, Mark Fristad, Charlene Miller, Brian Schneider, Michael Morrissey, Linda Wechsler, Catherine Durham, Cindy Lederer, Alex Stone and Chef Leif Eric Benson for their contributions to the development and release of Purple Pelisse. We also thank our industry cooperators, our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Washington, and Oregon potato commissions. The Oregon Agricultural Experiment Station, the Oregon Potato Commission, the Potato Variety Management Institute (PVMI), the USDA Cooperative State Research, Education, and Extension Service, and the USDA Agricultural Research Service provided partial financial support for this research. NR 15 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2012 VL 89 IS 4 BP 306 EP 314 DI 10.1007/s12230-012-9254-3 PG 9 WC Agronomy SC Agriculture GA 973CF UT WOS:000306329600007 ER PT J AU Crouter, SE DellaValle, DM Haas, JD AF Crouter, Scott E. DellaValle, Diane M. Haas, Jere D. TI Relationship between physical activity, physical performance, and iron status in adult women SO APPLIED PHYSIOLOGY NUTRITION AND METABOLISM-PHYSIOLOGIE APPLIQUEE NUTRITION ET METABOLISME LA English DT Article DE maximal aerobic capacity; iron depletion; accelerometry; endurance; efficiency; hemoglobin ID PREVIOUSLY UNTRAINED WOMEN; NONANEMIC WOMEN; DEFICIENCY ANEMIA; GAS-EXCHANGE; SUPPLEMENTATION; RATS; ACCELEROMETER; ADAPTATION; ENDURANCE; CAPACITY AB Iron deficiency affects approximately 16% of US females 18-45 years old. Iron is a key component of heme-containing proteins, which are essential for oxygen transport throughout the body. With low iron levels, performance and intense physical activity may be compromised. Thus, the purpose of this study was to examine the relationship between iron status, physical performance, and physical activity in 18- to 45-year-old females. Participants (N = 109) were screened for iron status using a venous blood sample, had their height and mass measured, and self-reported their physical activity level. The screening was used to match iron-depleted nonanemic females (hemoglobin, Hgb > 120 g.L-1; serum ferritin, sFer < 20 mu g.L-1) to females with normal iron levels. After participant matching, they had their body composition measured, performed three cycle ergometer tests (maximal, endurance, and efficiency), and wore an ActiGraph GT1M accelerometer for five consecutive days, except when sleeping or during water activities. The final sample consisted of 25 iron-depleted participants and 24 with normal iron levels. Key findings were as follows: (i) after controlling for fat-free mass and vigorous physical activity, iron-depleted females had a significantly lower (V) over dotO(2) at ventilatory threshold compared with those with normal iron levels (P < 0.05); and (ii) after controlling for age, iron-depleted females spent significantly more time in sedentary behaviors and significantly less time in light physical activity than those with normal iron levels (P < 0.05). The increased sedentary time in iron-depleted females may contribute to excess mass gain over time; however, further investigation is needed to confirm these results. C1 [Crouter, Scott E.; Haas, Jere D.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [DellaValle, Diane M.] Cornell Univ, USDA, ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Crouter, SE (reprint author), Univ Massachusetts Boston, Dept Exercise & Hlth Sci, 100 Morrissey Blvd, Boston, MA 02125 USA. EM scott.crouter@umb.edu RI Crouter, Scott/H-4852-2011; OI Crouter, Scott/0000-0003-1297-9538; DellaValle, Diane/0000-0002-3855-432X FU Cornell University's Division of Nutritional Sciences; N.S. Meinig Professorship FX This study was supported by Cornell University's Division of Nutritional Sciences and the N.S. Meinig Professorship. NR 33 TC 5 Z9 6 U1 2 U2 21 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1715-5312 J9 APPL PHYSIOL NUTR ME JI Appl. Physiol. Nutr. Metab. PD AUG PY 2012 VL 37 IS 4 BP 697 EP 705 DI 10.1139/H2012-044 PG 9 WC Nutrition & Dietetics; Physiology; Sport Sciences SC Nutrition & Dietetics; Physiology; Sport Sciences GA 970EK UT WOS:000306110700012 PM 22624679 ER PT J AU Rawles, SD Green, BW Gaylord, TG Barrows, FT McEntire, ME Freeman, DW AF Rawles, Steven D. Green, Bartholomew W. Gaylord, T. Gibson Barrows, Frederic T. McEntire, Matthew E. Freeman, Donald W. TI Response of sunshine bass (Morone chrysops x M. saxatilis) to digestible protein/dietary lipid density and ration size at summer culture temperatures in the Southern United States SO AQUACULTURE LA English DT Article DE Hybrid striped bass; Ration size; Protein and energy utilization; Temperature ID EUROPEAN SEA-BASS; HYBRID STRIPED BASS; SALMO-SALAR L; DICENTRARCHUS-LABRAX; WATER TEMPERATURE; AMMONIA EXCRETION; ATLANTIC SALMON; RAINBOW-TROUT; NUTRIENT RETENTION; OXYGEN-CONSUMPTION AB Temperature and ammonia levels often increase dramatically in ponds during summer production of sunshine bass and summer temperatures are projected to increase in the Southern US. Extended periods of high ammonia result in fish stress, disease, mortality and significant loss of feeding days as producers attempt to reduce ammonia to manageable levels through reduced feeding or lower protein diets. A factorial feeding trial was conducted in temperature controlled tanks to investigate main and interactive effects of three digestible protein (DP) levels (33, 40, 47%), two lipid levels (10, 18%) and two ration levels (full fed: satiation, restricted: 80% of satiation) on growth, body composition, nutrient and amino acid retention, and ammonia and phosphorus excretion in sunshine bass (mean weight: 75 g) reared at elevated temperature (30.5 +/- 0.5 degrees C). Diets were balanced on an available amino acid basis to the profile of hybrid striped bass muscle and supplemented with lysine and methionine at the equivalent of 330, 400, or 470 g/kg of muscle protein. Each DP x lipid x ration treatment was fed to triplicate tanks of fish for 116 days. All measured responses were significantly altered by main and interactive effects, but the patterns of interaction were similar among responses. Restricted feeding resulted in much lower final weights and weight gains but much higher ammonia excretion as a function of feed or N fed and body weight (BW), regardless of DP level. Lower dietary fat (10%) resulted in lower weight gains and poorer feed conversions as well as higher ammonia excretion (per g N fed/kg BW) regardless of DP or ration level. Weight gain (475%) and final fish weight (434 g) were highest at 47% DP/18% dietary lipid, but feed conversion, protein, energy and amino acid retention efficiencies were markedly poorer in the 47% DP diets regardless of lipid level due to hyperphagia in fish fed this diet. The 40/18 diet consistently outperformed the 33/18 diet in better growth and lower ammonia excretion as a function of N fed/BW, and nearly equaled the growth attained by fish fed 47/18 diet. Increasing ration to satiation at elevated temperature resulted in much greater improvements in weight gain, final weight and ammonia excretion of fish fed the 40/18 diet, as opposed to those fed the 33/18 diet. Amino acid retentions were nearly equal between the 33/18 and 40/18 diets and restricting feed to 80% of satiation slightly improved feed conversions and protein and amino acid retentions. Consistent lipid x DP interactions generally indicated that the differences among responses to DP level seen at 18% dietary lipid significantly decreased or disappeared at 10% dietary lipid. Phosphorus excretion was low and not significantly altered by main effects. Results suggest that a producer desiring to reduce pond ammonia with the least compromise to production efficiency would be better served by feeding a 40% DP/18% lipid diet at a reduced level instead of switching to a lower protein diet. Published by Elsevier B.V. C1 [Rawles, Steven D.; Green, Bartholomew W.; McEntire, Matthew E.; Freeman, Donald W.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Gaylord, T. Gibson] US Fish & Wildlife Serv, US Dept Interior, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Barrows, Frederic T.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. RP Rawles, SD (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM steven.rawles@ars.usda.gov OI Gaylord, Gibson/0000-0003-3037-0451 FU USDA/ARS [6225-31630-006-00D] FX Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This study was funded by the USDA/ARS under project number 6225-31630-006-00D. USDA is an equal opportunity provider and employer. NR 42 TC 3 Z9 3 U1 0 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD AUG 1 PY 2012 VL 356 BP 80 EP 90 DI 10.1016/j.aquaculture.2012.05.033 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 970ZK UT WOS:000306171100011 ER PT J AU Wacyk, J Powell, M Rodnick, K Overturf, K Hill, RA Hardy, R AF Wacyk, Jurij Powell, Madison Rodnick, Kenneth Overturf, Ken Hill, Rodney A. Hardy, Ronald TI Dietary protein source significantly alters growth performance, plasma variables and hepatic gene expression in rainbow trout (Oncorhynchus mykiss) fed amino acid balanced diets SO AQUACULTURE LA English DT Article DE Fishmeal; Plant protein; Soy protein isolate; Branched chain amino acids; Gene expression; TOR ID SALMON SALMO-SALAR; ASPARAGINE SYNTHETASE GENE; SENSING RESPONSE PATHWAY; FISH-MEAL; NITROGEN-EXCRETION; TOTAL REPLACEMENT; SOMATOTROPIC AXIS; MAMMALIAN TARGET; SOYBEAN PROTEIN; PLANT-PRODUCTS AB The objective of the study was to evaluate the effect of dietary protein source on fish growth, nutrient utilization, plasma variables and hepatic gene expression in juvenile rainbow trout (Oncorhynchus mykiss). Fishmeal (FM) and soy protein isolate (SPI) were used as the main sources of protein in six isonitrogenous, isolipidic and isocaloric diets. The amino acid profiles of the diets were completely balanced to minimize differences between experimental treatments and formulated to contain increasing levels of branched-chain amino acids (BCAA) based upon dietary requirements for trout (NRC, 1993). Dietary protein source more consistently changed the measured variables while BCAA supplementation had an unexpected effect over whole body lipid content. Growth performance and protein retention efficiency were significantly reduced in fish fed SPI diets independently of BCAA supplementation. Total concentration of amino acids as well as circulating indispensable amino acids (IAA) were significantly elevated in the plasma of fish receiving SPI diets compared to fish fed FM diets. The change in IAAwas large enough to increase (p<0.05) plasma IAA/DAA (DAA: dispensable amino acids) proportion even when the diets were formulated to have a ratio close to 1. Levels of circulating BCAA and alanine were also elevated in the fish fed SPI diets, possibly indicating a change in protein turnover. The use of SPI caused a reduction (p<0.05) in the hepatic expression levels of alanine amino transferase (alt1) and glutamine synthetase 2 (gls02), while an increase was observed for aspartate aminotransferase (got2), and asparagine synthetase (asns) compared with FM diets. Expression of the gene tor (target of rapamycin) declined over time for all treatments, while expression of a gene known to repress tor function, redd-1, was consistently higher in the liver of fish fed SPI diets. Glucose 6 phosphate dehydrogenase (g6pd) also showed a significantly higher expression in the liver of fish fed SPI diets but only at higher levels of BCAA supplementation. In summary dietary protein source has a significant effect over growth performance, body composition and hepatic gene expression in rainbow trout. We also identified for the first time in fish changes in the expression of redd-1, which may represent another regulatory point in the TOR cascade. (C) 2012 Elsevier B. V. All rights reserved. C1 [Wacyk, Jurij] Univ Chile, Fac Ciencias Agron, Dept Anim Prod, Santiago, Chile. [Powell, Madison; Hardy, Ronald] Univ Idaho, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Hill, Rodney A.] Univ Idaho, Dept Anim & Vet Sci, Hagerman, ID 83332 USA. [Overturf, Ken] USDA ARS, Trout Grains Project, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Rodnick, Kenneth] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. RP Wacyk, J (reprint author), Univ Chile, Fac Ciencias Agron, Dept Anim Prod, Casilla 1004, Santiago, Chile. EM jwacyk@u.uchile.cl RI Wacyk, Jurij/B-6544-2014; Powell, Madison/H-7050-2014 OI Wacyk, Jurij/0000-0002-9044-2755; Powell, Madison/0000-0002-1851-3168 NR 76 TC 21 Z9 22 U1 1 U2 59 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD AUG 1 PY 2012 VL 356 BP 223 EP 234 DI 10.1016/j.aquaculture.2012.05.013 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 970ZK UT WOS:000306171100030 ER PT J AU Liu, D Yan, SC Liu, D Huang, YP Tan, AJ Stanley, DW Song, QS AF Liu, Dan Yan, Shanchun Liu, Dan Huang, Yongping Tan, Anjiang Stanley, David W. Song, Qisheng TI GENETIC TRANSFORMATION MEDIATED BY piggyBac IN THE ASIAN CORN BORER, Ostrinia furnacalis (LEPIDOPTERA: CRAMBIDAE) SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Ostrinia furnacalis; piggyBac; excision assay; enhanced green fluorescent protein (EGFP); transgene ID STERILE INSECT TECHNIQUE; GERM-LINE TRANSFORMATION; CODLING MOTH; TRANSPOSON; VECTOR AB The Asiancorn borer, Ostrinia furnacalis, is a serious pest of corn, sorghum, and cotton in China and other Asian countries. The present study is the first attempt to establish the transgenic line in O. furnacalis using a piggyBac transposon, which will shed light on the future genetic control of O. furnacalis. A piggyBac vector pBac[A3EGFP] was constructed to express enhanced green fluorescence protein (EGFP)under the control of Bombyx mori actin3 promoter. Transient EGFP expression was detected 48 h after preblastodermic microinjection of pBac[A3EGFP] and the excision assay showed the transgenic vector was precisely excised. In G1 animals, PCR (polymerase chain reaction)-based investigations revealed that the exogenous gene had been introduced into O. furnacalis genome and expressed at the transcriptional level. Western blot analysis showed EGFP expression at the protein level, indicating the heritability of the transgene. C1 [Liu, Dan; Yan, Shanchun] NE Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China. [Liu, Dan; Huang, Yongping; Tan, Anjiang] Chinese Acad Sci, Res Ctr Insect Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai, Peoples R China. [Stanley, David W.] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. [Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO USA. RP Yan, SC (reprint author), Hexing Rd 26, Harbin 150040, Heilongjiang Pr, Peoples R China. EM yanshanchun@nefu.edu.cn; bom-byxtan@gmail.com FU National Natural Science Foundation of China [31030060, 30825007] FX Grant sponsor: National Natural Science Foundation of China; Grant numbers: 31030060, 30825007. NR 21 TC 4 Z9 5 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD AUG PY 2012 VL 80 IS 3 BP 140 EP 150 DI 10.1002/arch.21035 PG 11 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 972IU UT WOS:000306271600002 PM 22696097 ER PT J AU Chen, Q Marshall, MN Geib, SM Tien, M Richard, TL AF Chen, Qin Marshall, Megan N. Geib, Scott M. Tien, Ming Richard, Tom L. TI Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover SO BIORESOURCE TECHNOLOGY LA English DT Article DE Laccase; Delignification; TMAH-GC-MS; Enzymatic hydrolysis; Ensilage ID TETRAMETHYLAMMONIUM HYDROXIDE TMAH; IN-SITU; WHEAT-STRAW; PRETREATMENT; FERMENTATION; DEGRADATION; ENZYMES; PRODUCTS; REMOVAL; STORAGE AB The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations. (C) 2012 Published by Elsevier Ltd. C1 [Chen, Qin; Marshall, Megan N.; Richard, Tom L.] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. [Geib, Scott M.] ARS, USDA, Basin Agr Res Ctr, Hilo, HI 96720 USA. [Tien, Ming] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. RP Richard, TL (reprint author), Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. EM trichard@psu.edu RI Richard, Tom/H-5058-2012; OI Geib, Scott/0000-0002-9511-5139 FU USDA-DOE Biomass Research and Development Initiative [68-3A75-4-137]; Pennsylvania Agricultural Experiment Station FX The authors thank Dr. Bob Minard sincerely for assisting with TMAH-GC-MS analysis; Professors Ali Demirci and Dawn Luthe for lab support; and Rosa Jarvis for laboratory assistance. Funding was provided by the USDA-DOE Biomass Research and Development Initiative (contract # 68-3A75-4-137) and the Pennsylvania Agricultural Experiment Station. NR 34 TC 31 Z9 31 U1 5 U2 88 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD AUG PY 2012 VL 117 BP 186 EP 192 DI 10.1016/j.biortech.2012.04.085 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 971MV UT WOS:000306208900026 PM 22613895 ER PT J AU Moore, MT Locke, MA AF Moore, M. T. Locke, M. A. TI Phytotoxicity of Atrazine, S-Metolachlor, and Permethrin to Typha latifolia (Linneaus) Germination and Seedling Growth SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article DE Pesticides; Seed; Broad-leaf cattail ID TOXICITY; WATER; PESTICIDES; SIMAZINE; CABBAGE; TESTS; ROOT AB Phytotoxicity assessments were performed to compare responses of Typha latifolia (L.) seeds to atrazine (only) and atrazine + S-metolachlor exposure concentrations of 0.03, 0.3, 3, and 30 mg L-1, as well as permethrin exposure concentrations of 0.008, 0.08, 0.8, and 8 mg L-1. All atrazine + S-metolachlor exposures resulted in significantly reduced radicle development (p < 0.001). A stimulatory effect for coleoptile development was noted in the three highest atrazine (only) exposures (p = 0.0030, 0.0181, and 0.0016, respectively). This research provides data concerning the relative sensitivity of T. latifolia seeds to pesticides commonly encountered in agricultural settings, as well as critical understanding and development of using T. latifolia in phytoremediation efforts for pesticide exposures. C1 [Moore, M. T.; Locke, M. A.] USDA ARS, Water Qual & Ecol Res Unit, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Moore, MT (reprint author), USDA ARS, Water Qual & Ecol Res Unit, Natl Sedimentat Lab, POB 1157, Oxford, MS 38655 USA. EM matt.moore@ars.usda.gov NR 20 TC 3 Z9 3 U1 4 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-4861 J9 B ENVIRON CONTAM TOX JI Bull. Environ. Contam. Toxicol. PD AUG PY 2012 VL 89 IS 2 BP 292 EP 295 DI 10.1007/s00128-012-0682-z PG 4 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 972NM UT WOS:000306283800014 PM 22653305 ER PT J AU Thornton, J AF Thornton, Janey TI Making Our Schools "The Heart of Health" SO CHILDHOOD OBESITY LA English DT Editorial Material C1 USDA, Washington, DC 20250 USA. RP Thornton, J (reprint author), USDA, 1400 Independence Ave,SW Suite 219E, Washington, DC 20250 USA. EM Janey.thornton@osec.usda.gov NR 5 TC 1 Z9 1 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD AUG PY 2012 VL 8 IS 4 SI SI BP 278 EP 279 DI 10.1089/chi.2012.0080 PG 2 WC Pediatrics SC Pediatrics GA V32JJ UT WOS:000208947200003 PM 22867063 ER PT J AU Guthrie, JF Newman, C Ralston, K Prell, M Ollinger, M AF Guthrie, Joanne F. Newman, Constance Ralston, Katherine Prell, Mark Ollinger, Michael TI Understanding School Food Service Characteristics Associated with Higher Competitive Food Revenues Can Help Focus Efforts To Improve School Food Environments SO CHILDHOOD OBESITY LA English DT Article AB Many school food services sell extra foods and beverages, popularly referred to as "competitive foods," in addition to USDA school meals. On the basis of national survey data, most competitive foods and beverages selected by students are of low nutritional value. Recent federal legislation will allow schools that participate in USDA school meal programs to sell competitive foods only if the food items they sell meet nutrition standards based on the Dietary Guidelines for Americans. Concerns have been raised about the potential effects of limiting competitive foods on local school food service finances. However, national data indicate that only in a subset of schools do food services receive large amounts of revenues from competitive foods. These food services are typically located in secondary schools in more affluent districts, serving higher proportions of students who do not receive free or reduced-price meals. Compared to other food services, these food services couple higher competitive food revenues with lower school meal participation. Increasing school meal participation could increase meal revenues to offset any loss of competitive food revenues. Replacing less-healthful competitive items with healthier options could also help maintain school food service revenues while improving the school food environment. Nationally consistent nutrition standards for competitive foods may encourage development and marketing of healthful products. C1 [Guthrie, Joanne F.; Newman, Constance; Ralston, Katherine; Prell, Mark] Econ Res Serv, Food Assistance Branch, USDA, Washington, DC 20250 USA. [Ollinger, Michael] Econ Res Serv, Diet Safety & Hlth Econ Branch, USDA, Washington, DC 20250 USA. RP Guthrie, JF (reprint author), Econ Res Serv, Food Assistance Branch, USDA, 1400 Independence Ave SW,Mail Stop 1800, Washington, DC 20250 USA. EM jguthrie@ers.usda.gov NR 15 TC 3 Z9 3 U1 2 U2 12 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD AUG PY 2012 VL 8 IS 4 SI SI BP 298 EP 304 DI 10.1089/chi.2012.0062 PG 7 WC Pediatrics SC Pediatrics GA V32JJ UT WOS:000208947200007 PM 22867067 ER PT J AU Pojednic, RM Clark, DJ Patten, C Reid, K Phillips, EM Fielding, RA AF Pojednic, Rachele M. Clark, David J. Patten, Carolynn Reid, Kieran Phillips, Edward M. Fielding, Roger A. TI The specific contributions of force and velocity to muscle power in older adults SO EXPERIMENTAL GERONTOLOGY LA English DT Article DE Power; Velocity; Strength; Aging; Function ID CONTRACTILE PROPERTIES; PHYSICAL PERFORMANCE; SKELETAL-MUSCLE; ELDERLY-WOMEN; MEN; STRENGTH; MOBILITY; YOUNG; ASSOCIATION; ACTIVATION AB The purpose of this study is to examine the relative importance of the force-based and velocity-based measures of muscle performance to explain inter-individual differences in power production capability and functional task performance. Participants included seventy-nine men and women: middle-aged healthy adults (MH: 40-55 years), older healthy adults (OH: 70-85 years), and older adults with mobility limitations (OML: 70-85 years). Muscle power at 180 degrees/s, isometric maximal torque, and maximal contraction velocity at 40% 1RM were measured during unilateral leg extension. The Short Physical Performance Battery (SPPB) was used to differentiate between healthy and mobility limited older adults. Functional task performance was assessed using multiple chair rise and stair climb tests. Leg extensor force (torque), but not maximal contraction velocity, was significantly associated with muscle power in MH. Both torque and velocity were significantly associated with muscle power in OH. Maximal velocity, but not torque, was associated with power in OML. Maximal velocity demonstrated an association with multiple chair rise time and stair climb time in OML, but not MH or OH. It is concluded that movement velocity is an increasingly important determinant of maximal power output with advancing age. Furthermore, movement velocity is also a critical component of functional task performance with aging and may contribute to functional deficits. These findings help to explain why the rate-dependent variable power has emerged as a critical component of both assessment and rehabilitation of muscular performance and physical function in older adults. (C) 2012 Published by Elsevier Inc. C1 [Pojednic, Rachele M.; Reid, Kieran; Phillips, Edward M.; Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Clark, David J.; Patten, Carolynn] Malcom Randall VA Med Ctr, Brain Rehabil Res Ctr, Gainesville, FL USA. [Clark, David J.] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL USA. [Patten, Carolynn] Univ Florida, Dept Phys Therapy, Gainesville, FL USA. [Phillips, Edward M.] Harvard Univ, Sch Med, Dept Phys Med & Rehabil, Boston, MA USA. [Phillips, Edward M.] Spaulding Rehabil Hosp, Boston, MA USA. RP Fielding, RA (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM roger.fielding@tufts.edu RI Clark, David/A-4916-2013; Patten, Carolynn/B-4804-2009; OI Patten, Carolynn/0000-0002-9948-0045; Pojednic, Rachele/0000-0002-6117-0551 FU National Institute on Aging [AG18844]; U.S. Department of Agriculture [58-1950-7-707]; Boston Claude D. Pepper Older American Independence Center [1P30AG031679]; Boston Rehabilitation Outcomes Center; NIH [1R24HD065688-01A1]; NHLBI [T32HL69772] FX This research was supported by the National Institute on Aging grant number AG18844 and based upon work supported by the U.S. Department of Agriculture, under agreement 58-1950-7-707. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. This research was also supported by the Boston Claude D. Pepper Older American Independence Center 1P30AG031679, the Boston Rehabilitation Outcomes Center, funded by NIH Infrastructure Grant (1R24HD065688-01A1) and the NHLBI Training Grant (T32HL69772). NR 29 TC 23 Z9 23 U1 3 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0531-5565 J9 EXP GERONTOL JI Exp. Gerontol. PD AUG PY 2012 VL 47 IS 8 BP 608 EP 613 DI 10.1016/j.exger.2012.05.010 PG 6 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA 970CU UT WOS:000306106500009 PM 22626972 ER PT J AU Scalcinati, G Otero, JM Van Vleet, JRH Jeffries, TW Olsson, L Nielsen, J AF Scalcinati, Gionata Otero, Jose Manuel Van Vleet, Jennifer R. H. Jeffries, Thomas W. Olsson, Lisbeth Nielsen, Jens TI Evolutionary engineering of Saccharomyces cerevisiae for efficient aerobic xylose consumption SO FEMS YEAST RESEARCH LA English DT Article DE directed evolution; metabolic engineering; xylose; Saccharomyces cerevisiae; transcriptomics ID PENTOSE-PHOSPHATE PATHWAY; ETHANOL-PRODUCTION; PICHIA-STIPITIS; GENE-EXPRESSION; TRANSCRIPTIONAL REGULATION; XYLITOL DEHYDROGENASE; CHEMOSTAT CULTURES; REDUCTASE-ACTIVITY; GENOME DATABASE; CYTOSOLIC NADH AB Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S similar to cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S similar to cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S similar to cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up-regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source. C1 [Scalcinati, Gionata; Otero, Jose Manuel; Olsson, Lisbeth; Nielsen, Jens] Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden. [Scalcinati, Gionata; Otero, Jose Manuel; Olsson, Lisbeth; Nielsen, Jens] Tech Univ Denmark, Dept Syst Biol, Ctr Microbial Biotechnol, DK-2800 Lyngby, Denmark. [Van Vleet, Jennifer R. H.; Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Jeffries, Thomas W.] Forest Prod Lab, Inst Microbial & Biochem Technol, Madison, WI USA. RP Olsson, L (reprint author), Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden. EM lisbeth.olsson@chalmers.se RI Jeffries, Thomas/I-8576-2012; OI Jeffries, Thomas/0000-0001-7408-4065; Nielsen, Jens/0000-0002-9955-6003 FU Division of Bioprocess Research & Development, Merck Research Labs, Merck Co., Inc.; Chalmers Foundation; Knut and Alice Wallenberg Foundation FX J.M.O. is a Merck Doctoral Fellow and acknowledges financial support from the Division of Bioprocess Research & Development, Merck Research Labs, Merck & Co., Inc. Part of this research has been financed by the Chalmers Foundation and the Knut and Alice Wallenberg Foundation. NR 92 TC 31 Z9 33 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1567-1356 J9 FEMS YEAST RES JI FEMS Yeast Res. PD AUG PY 2012 VL 12 IS 5 BP 582 EP 597 DI 10.1111/j.1567-1364.2012.00808.x PG 16 WC Biotechnology & Applied Microbiology; Microbiology; Mycology SC Biotechnology & Applied Microbiology; Microbiology; Mycology GA 971FQ UT WOS:000306189600008 PM 22487265 ER PT J AU Yeh, HY Klesius, PH AF Yeh, Hung-Yueh Klesius, Phillip H. TI Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque) SO FISH PHYSIOLOGY AND BIOCHEMISTRY LA English DT Article DE Channel catfish; Ictalurus punctatus; Protein arginine methyltransferases; Arginine methylation; PRMT ID SUBSTRATE-SPECIFICITY; N-METHYLTRANSFERASE; SEQUENCE-ANALYSIS; METHYLATION; IDENTIFICATION; PRMT5; TRANSCRIPTION; BINDING; CDNA; FISH AB Protein arginine methylation, catalyzed by protein arginine methyltransferases (PRMT), has recently emerged as an important modification in the regulation of gene expression. In this communication, we identified and characterized the channel catfish orthologs to human PRMT 1, 3, 4 and 5, and PRMT4 like. Each PRMT nucleic acid sequence has an open reading frame (ORF) and 3'-untranslated regions. Each ORF appears to encode 361, 587 and 458 amino acid residues for PRMT1, PRMT4 and variant, respectively. The partial ORF of PRMT3 and PRMT5 encode 292 and 563 amino acids, respectively. By comparison with the human counterparts, each channel catfish PRMT also has conserved domains. For expression profile, the channel catfish PRMT1 transcript was detected by RT-PCR in spleens, anterior kidneys, livers, intestines, skin and gills of fish examined. Except in liver, the PRMT3 transcript was detected in all catfish tissues examined. However, the PRMT4 cDNA was detected in livers from all three catfish and gills from two fish, but not other tissues. This information will enable us to further elucidate PRMT functions in channel catfish. C1 [Yeh, Hung-Yueh] ARS, Poultry Microbiol Safety Res Unit, USDA, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. [Yeh, Hung-Yueh; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Yeh, HY (reprint author), ARS, Poultry Microbiol Safety Res Unit, USDA, Richard B Russell Agr Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS [6420-32000-024-00D] FX We are grateful to Mr. Jason White of the USDA ARS Aquatic Animal Health Research Unit, Auburn, AL, for excellent technical support, and Dr. Brian E. Scheffler and his Bioinformatics Group at the USDA ARS Genomics and Bioinformatics Research Unit in Stoneville, MS, for DNA sequencing and bioinformatics. We also thank Dr. Brian B. Oakley of Poultry Microbiological Safety Research Unit, ARS, USDA, Athens, GA, for construction of the phylogenetic tree. This study was supported by the USDA Agricultural Research Service CRIS project no. 6420-32000-024-00D. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 58 TC 1 Z9 1 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-1742 J9 FISH PHYSIOL BIOCHEM JI Fish Physiol. Biochem. PD AUG PY 2012 VL 38 IS 4 BP 1083 EP 1098 DI 10.1007/s10695-011-9593-x PG 16 WC Biochemistry & Molecular Biology; Fisheries; Physiology SC Biochemistry & Molecular Biology; Fisheries; Physiology GA 968KP UT WOS:000305980200016 PM 22286871 ER PT J AU Hudec, JL Peterson, DL AF Hudec, Jessica L. Peterson, David L. TI Fuel variability following wildfire in forests with mixed severity fire regimes, Cascade Range, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Canopy fuel; Cascade Range; Fuel characteristic classification system; Mixed severity; Reburn; Surface fuel ID CHARACTERISTIC CLASSIFICATION-SYSTEM; CROWN FIRE; KLAMATH MOUNTAINS; CENTRAL COLORADO; WILDLAND FIRES; SITKA SPRUCE; LONG-TERM; BEHAVIOR; PINE; WASHINGTON AB Fire severity influences post-burn structure and composition of a forest and the potential for a future fire to burn through the area. The effects of fire on forests with mixed severity fire regimes are difficult to predict and interpret because the quantity, structure, and composition of forest fuels vary considerably. This study examines the relationship between fire severity and post-burn fuel characteristics in forests with mixed severity fire regimes. We sampled live and dead canopy and surface fuels across four fire severity classes on three wildfires that occurred on the east side of the Cascade Range, USA, in 2007 and 2008. We used empirical fuels data and stand structure and composition characteristics to calculate potential surface fire behavior for the four fire severity classes. Post-burn average canopy cover is 25-30% in the low severity class and <10% in the high severity class and ranges from 0 to 50% for all fires. All variables representing post-burn canopy fuels differ by fire severity class. The average loading of dead and down woody fuels <7.6 cm diameter and litter is 0.9-1.1 kg m(-2) in the low severity class and 0.6-0.8 kg m(-2) in the high severity class. Values for fuel loading variables span a wide range of values within and among fires, and substantial overlap exists among severity classes. Fire severity generally does not influence post-burn dead and down woody fuel loading. Estimates of potential fire behavior also cover wide ranges of values, particularly among fires. Flame lengths average 0.4-0.8 m in the low severity classes and 0.3-1.1 m in the high severity classes. The range of potential flame length values, modeled with a 16.1 km h(-1) midflame wind speed, varies by up to 2.0 m within a single severity class (0.6-2.6 m). Fire severity does influence potential fire behavior, but typically just one severity class differs from the other three classes. These results indicate that fire severity influences immediate (2-3 years) post-burn canopy fuels and potential fire behavior but does not influence dead and down surface fuel loading for the three fires studied. The wide ranges of values for the fuel components analyzed demonstrate the variability that is characteristic of forests with mixed severity fire regimes and emphasize the need to consider the natural heterogeneity of these forests in fire and fuels management. Quantification of post-burn fuel variability is critical for understanding the ecological significance of mixed severity fires and developing restoration strategies that emulate characteristics of the historical fire regime. Published by Elsevier B.V. C1 [Hudec, Jessica L.] US Forest Serv, Gifford Pinchot Natl Forest, Trout Lake, WA 98650 USA. [Hudec, Jessica L.] Univ Washington, Sch Environm & Forest Sci, Fire & Mt Ecol Lab, Seattle, WA 98195 USA. [Peterson, David L.] US Forest Serv, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Hudec, JL (reprint author), US Forest Serv, Gifford Pinchot Natl Forest, 2455 Highway 141, Trout Lake, WA 98650 USA. EM jhudec@fs.fed.us; peterson@fs.fed.us FU Fire and Environmental Research Applications Team; Pacific Northwest Research Station; Gifford Pinchot National Forest FX We thank Ernesto Alvarado, Louisa Evers, Susan Hummel, Patti Loesche, Roger Ottmar, and Clint Wright for reviewing drafts of this manuscript and providing critical feedback. Assistance and guidance from Susan Hummel enabled us to stay on track and provided a critical link between Forest Service and graduate research. Susan Prichard provided expert advice on the use of FCCS. Rob Norheim created Fig. 1. Paige Eagle helped with FCCS runs and data management. Maureen Kennedy offered advice on statistical procedures. Amanda Rau provided information on the GW Fire. The U.S. Forest Service supplied guidance, field assistance, and funding through the Fire and Environmental Research Applications Team, the Pacific Northwest Research Station, and the Gifford Pinchot National Forest. We offer special thanks to Julia Biermann, Janelle Deane, Will Meginley, Sven Pulsifer, Joe Restaino, Mike Tjoelker, and Spus Wilder for their time in the field and to Gail Bouchard, Janine Clayton, Tom DeMeo, and Nancy Ryke for providing continuous Forest Service support. NR 89 TC 5 Z9 6 U1 3 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 1 PY 2012 VL 277 BP 11 EP 24 DI 10.1016/j.foreco.2012.04.008 PG 14 WC Forestry SC Forestry GA 966TL UT WOS:000305860200002 ER PT J AU Woodall, CW Walters, BF Westfall, JA AF Woodall, C. W. Walters, B. F. Westfall, J. A. TI Tracking downed dead wood in forests over time: Development of a piece matching algorithm for line intercept sampling SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest inventory; Coarse woody debris; Carbon flux; Biomass; Line intersect sampling; Bioenergy ID UNITED-STATES; DEBRIS; INVENTORY; ENERGY AB Emerging questions from bioenergy policy debates have highlighted knowledge gaps regarding the carbon and biomass dynamics of individual pieces of coarse woody debris (CWD) across the diverse forest ecosystems of the US. Although there is a lack of long-term measurements of CWD across the diverse forest ecosystems of the US, there is an abundance of line intersect sampling (LIS) transects used for monitoring efforts such as fuel loadings. In order to provide an objective method for monitoring the carbon/biomass dynamics of individual CWD pieces for use with LIS, this study developed and tested a CWD piece matching algorithm for inventory plots where LIS was used to sample CWD at two points in time across the eastern US. Results indicated that a CWD piece matching algorithm may be constructed using three steps: (1) matching the location of each piece, (2) matching individual piece metrics (e.g., large-end diameter), and (3) scoring an index of many CWD attributes with adjustment by decay and measurement error (i.e., quality control tolerances). For most forest types in the US, this study's algorithm matched between 20% and 40% of CWD pieces over time (approximate to 5 years). The algorithm performed poorly in forests potentially disturbed by floods and/or with relatively high mean annual temperatures and subsequent fast decay rates. Due to this influence of decay, the algorithm attained low match rates for highly decayed or small-sized CWD pieces. The algorithm should not be used to estimate changes in carbon/biomass within a stock change accounting framework. However, the algorithm may provide a method to aggregate a subset of paired LIS CWD observations over time to inform CWD dynamics research at large-scales. Published by Elsevier By. C1 [Woodall, C. W.; Walters, B. F.] USDA Forest Serv, No Res Stn, Forest Inventory & Anal Program, St Paul, MN 55108 USA. [Westfall, J. A.] USDA Forest Serv, No Res Stn, Forest Inventory & Anal Program, Newtown Sq, PA 19073 USA. RP Woodall, CW (reprint author), USDA Forest Serv, No Res Stn, Forest Inventory & Anal Program, St Paul, MN 55108 USA. EM cwoodall@fs.fed.us OI Woodall, Christopher/0000-0001-8076-6214 NR 29 TC 4 Z9 4 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 1 PY 2012 VL 277 BP 196 EP 204 DI 10.1016/j.foreco.2012.04.031 PG 9 WC Forestry SC Forestry GA 966TL UT WOS:000305860200021 ER PT J AU Buday, R Baranowski, T Thompson, D AF Buday, Richard Baranowski, Tom Thompson, Debbe TI Fun and Games and Boredom SO GAMES FOR HEALTH JOURNAL LA English DT Review AB Serious videogames use entertainment to teach, train, or change behavior. What began in the 1970s as tentative attempts to create learning software is now a recognized videogame genre and an emerging health science. Although more research is needed, a growing body of literature suggests serious videogames can be effective. Support for serious videogames, however, is not universal. An informal Web search reveals numerous skeptics. Critics question serious videogames' entertainment value and, thus, their viability. "How can serious videogames attract and maintain players,'' the argument goes, "if they aren't as much fun as commercial titles, or even any fun at all?'' This article examines the argument that, to be effective, serious videogames should be overtly fun and comparable to commercial off-the-shelf videogames. It explores differences between game developer- and researcher-led projects and discusses ways serious videogames can avoid boring and alienating players. It concludes that direct comparisons between serious and commercial game entertainment values may be misdirected. C1 [Buday, Richard] Archimage Inc, Houston, TX 77006 USA. [Baranowski, Tom; Thompson, Debbe] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. RP Buday, R (reprint author), Archimage Inc, 4203 Montrose Blvd,Suite 390, Houston, TX 77006 USA. EM RBuday@Archimage.com OI Baranowski, Tom/0000-0002-0653-2222 NR 40 TC 16 Z9 16 U1 0 U2 1 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD AUG PY 2012 VL 1 IS 4 BP 257 EP 261 DI 10.1089/g4h.2012.0026 PG 5 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA V40YQ UT WOS:000209514300006 ER PT J AU Kramer, MG Sanderman, J Chadwick, OA Chorover, J Vitousek, PM AF Kramer, Marc G. Sanderman, Jonathan Chadwick, Oliver A. Chorover, Jon Vitousek, Peter M. TI Long-term carbon storage through retention of dissolved aromatic acids by reactive particles in soil SO GLOBAL CHANGE BIOLOGY LA English DT Article DE aromatic acids; carbon cycle science; climate change; dissolved organic carbon; long-term carbon stabilization; short range ordered minerals; soil carbon; soil minerals ID ORGANIC-MATTER CHEMISTRY; STATE C-13 NMR; GRASSLAND SOILS; MINERAL SOILS; 0.3-4100 KYR; LIGNIN; FOREST; CHRONOSEQUENCE; STABILIZATION; SEDIMENTS AB Soils retain large quantities of carbon, thereby slowing its return to the atmosphere. The mechanisms governing organic carbon sequestration in soil remain poorly understood, yet are integral to understanding soil-climate feedbacks. We evaluated the biochemistry of dissolved and solid organic carbon in potential source and sink horizons across a chronosequence of volcanic soils in Hawai'i. The soils are derived from similar basaltic parent material on gently sloping volcanic shield surfaces, support the same vegetation assemblage, and yet exhibit strong shifts in soil mineralogy and soil carbon content as a function of volcanic substrate age. Solid-state13carbon nuclear magnetic resonance spectra indicate that the most persistent mineral-bound carbon is comprised of partially oxidized aromatic compounds with strong chemical resemblance to dissolved organic matter derived from plant litter. A molecular mixing model indicates that protein, lipid, carbohydrate, and char content decreased whereas oxidized lignin and carboxyl/carbonyl content increased with increasing short-range order mineral content. When solutions rich in dissolved organic matter were passed through Bw-horizon mineral cores, aromatic compounds were preferentially sorbed with the greatest retention occurring in horizons containing the greatest amount of short-range ordered minerals. These minerals are reactive metastable nanocrystals that are most common in volcanic soils, but exist in smaller amounts in nearly all major soil classes. Our results indicate that long-term carbon storage in short-range ordered minerals occurs via chemical retention with dissolved aromatic acids derived from plant litter and carried along preferential flow-paths to deeper B horizons. C1 [Kramer, Marc G.] Univ Calif Santa Cruz, Dept Earth & Planetary Sci, Santa Cruz, CA 95064 USA. [Sanderman, Jonathan] CSIRO, Div Land & Water, Urrbrae, SA 5064, Australia. [Chadwick, Oliver A.] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Chorover, Jon] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Vitousek, Peter M.] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. RP Kramer, MG (reprint author), US Forest Serv, Portland, OR 97208 USA. EM mkramer@ucsc.edu RI Sanderman, Jonathan/C-3818-2011; CSIRO, SAF/H-3134-2013 OI Sanderman, Jonathan/0000-0002-3215-1706; FU National Research Initiative from the USDA National Institute of Food and Agriculture [2007-35107-18429] FX We thank Russell Johnson, Dyke Andreasen, and Ping Yu for assistance during with the soil core experiment, stable isotope analysis, and NMR experiments. We thank Heraldo Farrington for assistance with sampling soils in the field. Soil column leaching experiments, sample preparation and analyses were conducted at the University of California, Santa Cruz and the Western Regional Research Center of PWA/ARS/USDA/EIW. This work was financially supported by National Research Initiative grant no. 2007-35107-18429 from the USDA National Institute of Food and Agriculture. Initial support was provided to the lead author by NASA Ames Research Center. NR 61 TC 48 Z9 49 U1 11 U2 126 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD AUG PY 2012 VL 18 IS 8 BP 2594 EP 2605 DI 10.1111/j.1365-2486.2012.02681.x PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 971UB UT WOS:000306228300020 ER PT J AU Rosenthal, DM Slattery, RA Miller, RE Grennan, AK Cavagnaro, TR Fauquet, CM Gleadow, RM Ort, DR AF Rosenthal, David M. Slattery, Rebecca A. Miller, Rebecca E. Grennan, Aleel K. Cavagnaro, Timothy R. Fauquet, Claude M. Gleadow, Roslyn M. Ort, Donald R. TI Cassava about-FACE: Greater than expected yield stimulation of cassava (Manihot esculenta) by future CO2 levels SO GLOBAL CHANGE BIOLOGY LA English DT Article DE Africa; food security; harvest index; photosynthetic acclimation; sink strength; tropical crops ID TEMPERATURE RESPONSE FUNCTIONS; ATMOSPHERIC PARTIAL-PRESSURE; POTATO SOLANUM-TUBEROSUM; DRY-MATTER PRODUCTION; ELEVATED CO2; CARBON-DIOXIDE; ENRICHMENT FACE; LIMITED PHOTOSYNTHESIS; DOWN-REGULATION; FOOD SECURITY AB Globally, cassava is the second most important root crop after potatoes and the fifth most important crop overall in terms of human caloric intake. In addition to its growing global importance for feed, fuel, and starch, cassava has long been vital to food security in Sub-Saharan Africa. Climate change is expected to have its most severe impact on crops in food insecure regions, yet little is known about how cassava productivity will respond to climate change. The most important driver of climate change is globally increasing atmospheric CO2 concentration ([CO2]). However, the potential for cassava to enhance food security in an elevated [CO2] world is uncertain as greenhouse and open top chamber (OTC) study reports are ambiguous. Studies have yielded misleading results in the past regarding the effect of elevated [CO2] on crop productivity, particularly in cases where pots restricted sink growth. To resolve these conflicting results, we compare the response of cassava to growth at ambient (ca. 385 similar to ppm) and elevated [CO2] (585 similar to ppm) under field conditions and fully open air [CO2] elevation. After three and half months of growth at elevated [CO2], above ground biomass was 30% greater and cassava root tuber dry mass increased over 100% (fresh weight increased 89%). High photosynthetic rates and photosynthetic stimulation by elevated [CO2], larger canopies, and a large sink capacity all contributed to cassava's growth and yield stimulation. Cassava exhibited photosynthetic acclimation via decreased Rubisco capacity early in the season prior to root tuber initiation when sink capacity was smaller. Importantly, and in contrast to a greenhouse study, we found no evidence of increased leaf N or total cyanide concentration in elevated [CO2]. All of our results are consistent with theoretical expectations; however, the magnitude of the yield increase reported here surpasses all other C3 crops and thus exceeds expectations. C1 [Rosenthal, David M.; Ort, Donald R.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. [Slattery, Rebecca A.; Grennan, Aleel K.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Miller, Rebecca E.; Cavagnaro, Timothy R.] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia. [Cavagnaro, Timothy R.] Monash Univ, Australian Ctr Biodivers, Clayton, Vic 3800, Australia. [Fauquet, Claude M.] Donald Danforth Plant Sci Ctr, Int Lab Trop Agr Biotechnol, St Louis, MO USA. RP Ort, DR (reprint author), ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. EM d-ort@illinois.edu RI Rosenthal, David/C-9559-2012; OI Rosenthal, David/0000-0002-4822-5861; Cavagnaro, Timothy/0000-0002-9922-5677; Gleadow, Roslyn/0000-0003-4756-0411 FU USDA-ARS Global Change and Photosynthesis Research Unit FX We would like to thank Dr Dimuth Siritunga from the University of Mayaguez, Puerto Rico for providing the plant material. We are also grateful to Kannan Puthuval and David Drag for managing the SoyFACE site, Ursula Ruiz Vera, Sharon Gray, and Anna Locke for assisting in gas exchange measurements, and Nathan Couch, Alex Hargus, and Colleen Cahill for assisting during planting and sampling. This work was funded by the USDA-ARS Global Change and Photosynthesis Research Unit. NR 80 TC 21 Z9 21 U1 5 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD AUG PY 2012 VL 18 IS 8 BP 2661 EP 2675 DI 10.1111/j.1365-2486.2012.02726.x PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 971UB UT WOS:000306228300026 ER PT J AU Ji, L Wylie, BK Nossov, DR Peterson, B Waldrop, MP McFarland, JW Rover, J Hollingsworth, TN AF Ji, Lei Wylie, Bruce K. Nossov, Dana R. Peterson, Birgit Waldrop, Mark P. McFarland, Jack W. Rover, Jennifer Hollingsworth, Teresa N. TI Estimating aboveground biomass in interior Alaska with Landsat data and field measurements SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Aboveground biomass; Spectral vegetation index; Landsat; Lidar; Alaska; Yukon Flats ecoregion ID DIFFERENCE WATER INDEX; FOREST INVENTORY DATA; VEGETATION INDEXES; BOREAL FORESTS; ETM+ DATA; NORTHERN ALASKA; CARBON BALANCE; EOS-MODIS; REMOTE; TM AB Terrestrial plant biomass is a key biophysical parameter required for understanding ecological systems in Alaska. An accurate estimation of biomass at a regional scale provides an important data input for ecological modeling in this region. In this study, we created an aboveground biomass (AGB) map at 30-m resolution for the Yukon Flats ecoregion of interior Alaska using Landsat data and field measurements. Tree, shrub, and herbaceous AGB data in both live and dead forms were collected in summers and autumns of 2009 and 2010. Using the Landsat-derived spectral variables and the field AGB data, we generated a regression model and applied this model to map AGB for the ecoregion. A 3-fold cross-validation indicated that the AGB estimates had a mean absolute error of 21.8 Mg/ha and a mean bias error of 5.2 Mg/ha. Additionally, we validated the mapping results using an airborne lidar dataset acquired for a portion of the ecoregion. We found a significant relationship between the lidar-derived canopy height and the Landsat-derived AGB (R-2 = 0.40). The AGB map showed that 90% of the ecoregion had AGB values ranging from 10 Mg/ha to 134 Mg/ha. Vegetation types and fires were the primary factors controlling the spatial AGB patterns in this ecoregion. Published by Elsevier B.V. C1 [Ji, Lei; Peterson, Birgit] US Geol Survey, ASRC Res & Technol Solut, Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. [Wylie, Bruce K.; Rover, Jennifer] US Geol Survey, EROS Ctr, Sioux Falls, SD 57198 USA. [Nossov, Dana R.] Univ Alaska Fairbanks, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Waldrop, Mark P.; McFarland, Jack W.] US Geol Survey, Menlo Pk, CA 94025 USA. [Hollingsworth, Teresa N.] US Forest Serv, Boreal Ecol Cooperat Res Unit, Pacific NW Res Stn, USDA, Fairbanks, AK 99775 USA. RP Ji, L (reprint author), US Geol Survey, ASRC Res & Technol Solut, Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. EM lji@usgs.gov RI Wylie, Bruce/H-3182-2014; OI Wylie, Bruce/0000-0002-7374-1083; Waldrop, Mark/0000-0003-1829-7140; Rover, Jennifer/0000-0002-3437-4030 FU U.S. Geological Survey Climate Effects Network and Global Change Research & Development Programs; USGS [G08PC91508] FX This study was funded by the U.S. Geological Survey Climate Effects Network and Global Change Research & Development Programs. The work by Lei Ji and Birgit Peterson was performed under USGS contract G08PC91508. We thank Jason Stoker for processing the raw lidar data, and Mark Winterstein for assistance with field work. We also thank Eugene Fosnight, Roger Auch, and Tom Adamson for reviewing the manuscript and providing valuable comments. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 66 TC 31 Z9 32 U1 4 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD AUG PY 2012 VL 18 BP 451 EP 461 DI 10.1016/j.jag.2012.03.019 PG 11 WC Remote Sensing SC Remote Sensing GA 971IZ UT WOS:000306198900042 ER PT J AU Clotilde, LM Bernard, C Sequera, DE Karmali, A Fusellier, A Carter, JM AF Clotilde, Laurie M. Bernard, Clay Sequera, Dean E. Karmali, Anis Fusellier, Andrew Carter, J. Mark TI Dynex: Multiplex ELISA Technology SO JALA LA English DT Article DE ELISA; Dynex; automated; multiplex; Escherichia coli; Shiga toxin AB Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates. C1 [Clotilde, Laurie M.; Bernard, Clay; Carter, J. Mark] USDA ARS, Foodborne Contaminants Res Unit, Albany, CA 94710 USA. [Sequera, Dean E.; Karmali, Anis; Fusellier, Andrew] Dynex Technol, Chantilly, VA USA. RP Carter, JM (reprint author), USDA ARS, Foodborne Contaminants Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM j.mark.carter@ars.usda.gov RI Karmali, Amin/E-4787-2011; Carter, John Mark/K-2485-2015 OI Karmali, Amin/0000-0003-0419-401X; Carter, John Mark/0000-0001-8251-4168 NR 5 TC 1 Z9 1 U1 1 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 2211-0682 J9 JALA-J LAB AUTOM JI JALA PD AUG PY 2012 VL 17 IS 4 BP 309 EP 314 DI 10.1177/2211068211434156 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 972IJ UT WOS:000306270200008 PM 22357566 ER PT J AU Wang, K Zhang, J Zhu, ZQ Huang, HG Li, TQ He, ZL Yang, XE Alva, A AF Wang, Kai Zhang, Jie Zhu, Zhiqiang Huang, Huagang Li, Tingqiang He, Zhenli Yang, Xiaoe Alva, Ashok TI Pig manure vermicompost (PMVC) can improve phytoremediation of Cd and PAHs co-contaminated soil by Sedum alfredii SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Cadmium; Co-contamination; Hyperaccumulator; Organic fertilizer; Phytoremediation; Polycyclic aromatic hydrocarbons ID POLYCYCLIC AROMATIC-HYDROCARBONS; ZEA-MAYS L.; CADMIUM TOLERANCE; PLANT UPTAKE; PHYTOEXTRACTION; PYRENE; DISSIPATION; HYPERACCUMULATOR; PHENANTHRENE; DEGRADATION AB A major challenge to phytoremediation of co-contaminated soils is developing strategies for efficient and simultaneous removal of multiple pollutants. A pot experiment was conducted to investigate the potential for enhanced phytoextraction of cadmium (Cd) by Sedum alfredii and dissipation of polycyclic aromatic hydrocarbons (PAHs) in co-contaminated soil by application of pig manure vermicompost (PMVC). Soil contaminated by Cd (5.53 mg kg(-1) DW) was spiked with phenanthrene, anthracene, and pyrene together (250 mg kg(-1) DW for each PAH). A pot experiment was conducted in a greenhouse with four treatments: (1) soil without plants and PMVC (Control), (2) soil planted with S. alfredii (Plant), (3) soil amended with PMVC at 5 % (w/w) (PMVC), and (4) treatment 2 + 3 (Plant + PMVC). After 90 days, shoot and root biomass of plants, Cd concentrations in plant and soil, and PAH concentrations in soil were determined. Abundance of PAH degraders in soil, soil bacterial community structure and diversity, and soil enzyme activities and microbial biomass carbon were measured. Application of PMVC to co-contaminated soil increased the shoot and root dry biomass of S. alfredii by 2.27- and 3.93-fold, respectively, and simultaneously increased Cd phytoextraction without inhibiting soil microbial population and enzyme activities. The highest dissipation rate of PAHs was observed in Plant + PMVC treatment. However, neither S. alfredii nor PMVC enhanced PAH dissipation when applied separately. Abundance of PAH degraders in soil was not significantly related to PAH dissipation rate. Plant + PMVC treatment significantly influenced the bacterial community structure. Enhanced PAH dissipation in the Plant + PMVC treatment could be due to the improvement of plant root growth, which may result in increased root exudates, and subsequently change bacterial community structure to be favorable for PAH dissipation. This study demonstrated that remediation of Cd and PAHs co-contaminated soil by S. alfredii can be enhanced by simultaneous application of PMVC. Long-term evaluation of this strategy in co-contaminated field sites is needed. C1 [Wang, Kai; Zhang, Jie; Zhu, Zhiqiang; Huang, Huagang; Li, Tingqiang; Yang, Xiaoe] Zhejiang Univ, MOE Key Lab Environm Remediat & Ecosyst Hlth, Coll Environm & Resources Sci, Hangzhou 310058, Zhejiang, Peoples R China. [Zhu, Zhiqiang] Hainan Univ, Coll Agr, Haikou 570228, Peoples R China. [He, Zhenli] Univ Florida, Indian River Res & Educ Ctr, Inst Food & Agr Sci, Ft Pierce, FL 34945 USA. [Alva, Ashok] ARS, USDA, Prosser, WA 99350 USA. RP Li, TQ (reprint author), Zhejiang Univ, MOE Key Lab Environm Remediat & Ecosyst Hlth, Coll Environm & Resources Sci, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. EM litq@zju.edu.cn; xyang571@yahoo.com RI Zhang, Jie/E-4768-2013; He, Zhenli/R-1494-2016; Li, Tingqiang/B-2592-2017 OI Zhang, Jie/0000-0002-8344-5907; He, Zhenli/0000-0001-7761-2070; Li, Tingqiang/0000-0003-4448-4871 FU Ministry of Science of China [2012AA101405-1, 2009AA06Z316]; Ministry of Education of China [310003]; Ministry of Environmental Protection of China [2011467057]; Fundamental Research Funds for the Central Universities FX This study was financially supported by "863" Target Goal Projects from Ministry of Science of China (#2012AA101405-1; #2009AA06Z316), a key project from Ministry of Education of China (#310003), a project from Ministry of Environmental Protection of China (#2011467057) and Fundamental Research Funds for the Central Universities. NR 51 TC 11 Z9 11 U1 1 U2 67 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 J9 J SOIL SEDIMENT JI J. Soils Sediments PD AUG PY 2012 VL 12 IS 7 BP 1089 EP 1099 DI 10.1007/s11368-012-0539-4 PG 11 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA 971QF UT WOS:000306218200008 ER PT J AU Ham, C Champ, PA Loomis, JB Reich, RM AF Ham, Charlotte Champ, Patricia A. Loomis, John B. Reich, Robin M. TI Accounting for Heterogeneity of Public Lands in Hedonic Property Models SO LAND ECONOMICS LA English DT Article ID OPEN-SPACE; SPATIAL DEPENDENCE; IMPLICIT PRICES; VALUES; AMENITIES; FORESTS; IMPACT AB Open space lands, national forests in particular, are usually treated as homogeneous entities in hedonic price studies. Failure to account for the heterogeneous nature of public open spaces may result in inappropriate inferences about the benefits of proximate location to such lands. In this study the hedonic price method is used to estimate the marginal values for proximity to the Pike National Forest. The results indicate that specifying the forest as homogeneous overstates the benefits for homes within two miles relative to specifying the forest based on land use characteristics, because the significant negative effect from noise-intensive activities is omitted. C1 [Ham, Charlotte] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Champ, Patricia A.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO USA. [Loomis, John B.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [Reich, Robin M.] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. RP Ham, C (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. NR 42 TC 10 Z9 10 U1 0 U2 10 PU UNIV WISCONSIN PI MADISON PA SOCIAL SCIENCE BLDG, MADISON, WI 53706 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD AUG PY 2012 VL 88 IS 3 BP 444 EP 456 PG 13 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA 245QK UT WOS:000326478700002 ER PT J AU Muhidinov, Z Teshaev, K Jonmurodov, A Khalikov, D Fishman, M AF Muhidinov, Zayniddin Teshaev, Khurshed Jonmurodov, Abduvaly Khalikov, Djurabay Fishman, Marshall TI Physico-Chemical Characterization of Pectic Polysaccharides from Various Sources Obtained by Steam Assisted Flash Extraction (SAFE) SO MACROMOLECULAR SYMPOSIA LA English DT Proceedings Paper CT 14th IUPAC International Symposium on Macromolecular Complexes (MMC) CY AUG 14-17, 2011 CL Helsinki, FINLAND SP IUPAC DE aggregation; microgels; molar mass; molar mass distribution; pectin AB Pectic polysaccharides (PP) have been isolated from a variety of sources and characterized by their yield, anhydrogalacturonic (AGA) content, degree of methyl esterification (DE) and microgel (MG) content. Molar mass and mass distribution (MMD) were analyzed by high performance size-exclusion chromatography (HPSEC) coupled with pressure differential viscosity (PDV) and differential refractive index (DRI) detectors. Results were grouped according to the DE of the PP. Among sources of lower methylated (LM) pectins, apricot pectin had the highest weight average molar mass (M-w) followed by apple pectin. Among high methylated (HM) pectins, pumpkin pectin had the highest value of M-w followed by tangerine and lemon pectins. All pectins studied were found to have bi-modal distribution as indicated by their molar mass calibration curves. Apple pectin was the most polydisperse whereas pumpkin pectin was the least polydisperse as indicated by M-w/M-n, where M-n is the number average molar mass. C1 [Muhidinov, Zayniddin; Teshaev, Khurshed; Jonmurodov, Abduvaly; Khalikov, Djurabay] Tajikistan Acad Sci, Inst Chem, Lab High Mol Cpds, Dushanbe 734063, Tajikistan. [Fishman, Marshall] USDA ARS, Easter Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Muhidinov, Z (reprint author), Tajikistan Acad Sci, Inst Chem, Lab High Mol Cpds, 299-2 Ainy Str, Dushanbe 734063, Tajikistan. EM zayniddinm@yahoo.com NR 15 TC 2 Z9 2 U1 2 U2 5 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1022-1360 EI 1521-3900 J9 MACROMOL SYMP JI Macromol. Symp. PD AUG PY 2012 VL 317 IS 1 SI SI BP 142 EP 148 DI 10.1002/masy.201100108 PG 7 WC Polymer Science SC Polymer Science GA AI8OQ UT WOS:000337179400018 ER PT J AU Jackson, CR Furtula, V Farrell, EG Barrett, JB Hiott, LM Chambers, P AF Jackson, Charlene R. Furtula, Vesna Farrell, Erin G. Barrett, John B. Hiott, Lari M. Chambers, Patricia TI A Comparison of BOX-PCR and Pulsed-Field Gel Electrophoresis to Determine Genetic Relatedness of Enterococci from Different Environments SO MICROBIAL ECOLOGY LA English DT Article ID BACTERIAL SOURCE TRACKING; RESISTANT ENTEROCOCCI; ANTIBIOTIC-RESISTANCE; VANCOMYCIN-RESISTANT; TYPING SCHEME; FAECIUM; DIVERSITY; POLLUTION; PROFILES; CHICKENS AB Genetic relatedness of enterococci from poultry litter to enterococci from nearby surface water and groundwater in the Lower Fraser Valley regions of British Columbia, Canada was determined. A new automated BOX-PCR and Pulsed-Field Gel Electrophoresis (PFGE) were used to subtype enterococcal isolates from broiler and layer litter and surface and groundwater. All surface water samples (n = 12) were positive for enterococci, as were 11% (3/28) of groundwater samples. Enterococcus faecium (n = 90) was isolated from all sources, while Enterococcus faecalis (n = 59) was isolated from all sources except layer litter. The majority of E. faecalis originated from broiler litter (28/59; 47.5%) while the majority of E. faecium were isolated from layer litter (29/90; 32.2%). E. faecalis grouped primarily by source using BOX-PCR. Isolates from water samples were dispersed more frequently among PFGE groups containing isolates from poultry litter. E. faecium strains were genetically diverse as overall clustering was independent of source by both molecular methods. Subgroups of E. faecium isolates based upon source (layer litter) were present in BOX-PCR groups. Three individual E. faecalis groups and two individual E. faecium groups were 100% similar using BOX-PCR; only one instance of 100% similarity among isolates using PFGE was observed. Although enterococci from litter and water sources were grouped together using BOX-PCR and PFGE, isolates originating from water could not be definitively identified as originating from poultry litter. Automation of BOX-PCR amplicon separation and visualization increased the reproducibility and standardization of subtyping using this procedure. C1 [Furtula, Vesna] Environm Canada, Pacific Environm Sci Ctr, N Vancouver, BC V7H 1B1, Canada. [Jackson, Charlene R.; Barrett, John B.; Hiott, Lari M.] USDA ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30605 USA. [Furtula, Vesna; Farrell, Erin G.; Chambers, Patricia] Environm Canada, Aquat Ecosyst Impacts Res Div, N Vancouver, BC V7H 1B1, Canada. RP Furtula, V (reprint author), Environm Canada, Pacific Environm Sci Ctr, 2645 Dollarton Hwy, N Vancouver, BC V7H 1B1, Canada. EM vesna.furtula@ec.gc.ca FU Health Canada FX The authors are thankful to Health Canada for providing the funds for this research. Many thanks to Water Management and Indicators, Section of Environment Canada, for the help with sampling. The authors are also thankful to reviewers for reviewing the paper. NR 31 TC 4 Z9 4 U1 0 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 J9 MICROB ECOL JI Microb. Ecol. PD AUG PY 2012 VL 64 IS 2 BP 378 EP 387 DI 10.1007/s00248-012-0027-9 PG 10 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA 971AS UT WOS:000306174700009 PM 22383122 ER PT J AU Harder, CB Lodge, DJ Petersen, RH Hughes, KW Blanco, JC Froslev, TG Laessoe, T AF Harder, Christoffer Bugge Lodge, D. Jean Petersen, Ronald H. Hughes, Karen W. Cifuentes Blanco, Joaquin Froslev, Tobias Guldberg Laessoe, Thomas TI Amyloidity is not diagnostic for species in the Mycena pearsoniana complex (Mycena sectio Calodontes) SO MYCOLOGICAL PROGRESS LA English DT Article DE Mycena; ITS phylogeny; Amyloidity ID GEOGRAPHICALLY SEPARATED COLLECTIONS; INFRASPECIFIC VARIATION; PHYLOGENETIC ANALYSIS; NORTHERN-HEMISPHERE; FUNGI; DELIMITATION; RECOGNITION; PATTERNS; BASIDIOMYCOTA; AGARICALES AB In Mycena sectio Calodontes with otherwise amyloid spores, the inamyloid spores of Mycena pearsoniana Dennis ex Singer were a distinguishing feature for this species and its subsection Violacella. Although the original concept of this species was European, Singer chose to typify it with material collected in Mexico. The name has since been applied to all European collections with inamyloid spores and decurrent lamellae. Our phylogenetic analysis of 91 ITS sequences from European, North and South American Calodontes collections shows that European collections identified as M. pearsoniana fall into two well-supported sibling clades together with both inamyloid and weakly amyloid North American collections. Since the holotype of M. pearsoniana is in an advanced state of decay, we have selected an epitype from a North American locality with a climate comparable to the Mexican type locality. Our results show weakly and inamyloid spore reactions to be homoplastic in Calodontes, and furthermore that spores of M. pearsoniana can show either amyloid or inamyloid reactions interchangeably. This raises doubt about the taxonomic value of this trait in Mycena systematics. C1 [Harder, Christoffer Bugge] Univ Copenhagen, Dept Biol, DK-1353 Copenhagen K, Denmark. [Lodge, D. Jean] US Forest Serv, Ctr Forest Mycol Res, USDA, No Res Stn, Luquillo, PR 00773 USA. [Petersen, Ronald H.; Hughes, Karen W.] Univ Tennessee, Knoxville, TN 37996 USA. [Cifuentes Blanco, Joaquin] Univ Nacl Autonoma Mexico, Fac Ciencias, Mexico City 4510, DF, Mexico. [Froslev, Tobias Guldberg] Lab Filadelfia, DK-4293 Dianalund, Denmark. [Laessoe, Thomas] Univ Copenhagen, Dept Ecol & Evolut Biol, DK-2100 Copenhagen O, Denmark. RP Harder, CB (reprint author), Univ Copenhagen, Dept Biol, Oster Farimagsgade 2D, DK-1353 Copenhagen K, Denmark. EM cbharder@bio.ku.dk FU US National Science Foundation [DBI 6338699]; Danish Council for Strategic Research [2104-08-0012, MIRESOWA] FX We thank the curator of the LILOA herbarium in Argentina for loaning the holotype of M. pearsoniana for study. Collecting in the Great Smoky Mountain National Park was supported by US National Science Foundation grant DBI 6338699 to K. W. Hughes and R. H. Petersen at the University of Tennesse, Knoxville. C. B. Harder was supported by The Danish Council for Strategic Research grant (2104-08-0012, MIRESOWA) at the time of the writing. The authors thank B. A. Perry and the following pre-reviewers for valuable comments on previous versions of the manuscript: K. O'Donnell, J. Geml and D. E. Desjardin. NR 46 TC 3 Z9 4 U1 0 U2 9 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-416X J9 MYCOL PROG JI Mycol. Prog. PD AUG PY 2012 VL 11 IS 3 BP 725 EP 732 DI 10.1007/s11557-011-0782-x PG 8 WC Mycology SC Mycology GA 973FQ UT WOS:000306345100010 ER PT J AU Vicca, S Gilgen, AK Serrano, MC Dreesen, FE Dukes, JS Estiarte, M Gray, SB Guidolotti, G Hoeppner, SS Leakey, ADB Ogaya, R Ort, DR Ostrogovic, MZ Rambal, S Sardans, J Schmitt, M Siebers, M van der Linden, L van Straaten, O Granier, A AF Vicca, S. Gilgen, A. K. Serrano, M. Camino Dreesen, F. E. Dukes, J. S. Estiarte, M. Gray, S. B. Guidolotti, G. Hoeppner, S. S. Leakey, A. D. B. Ogaya, R. Ort, D. R. Ostrogovic, M. Z. Rambal, S. Sardans, J. Schmitt, M. Siebers, M. van der Linden, L. van Straaten, O. Granier, A. TI Urgent need for a common metric to make precipitation manipulation experiments comparable SO NEW PHYTOLOGIST LA English DT Letter DE drought stress; plant available water; precipitation manipulation experiments; soil moisture; synthesis ID TERRESTRIAL ECOSYSTEMS; RESPONSES; DYNAMICS; SOIL; CANOPY; GROWTH; CARBON; LEAF C1 [Vicca, S.; Serrano, M. Camino; Dreesen, F. E.] Univ Antwerp, Res Grp Plant & Vegetat Ecol, Dept Biol, B-2610 Antwerp, Belgium. [Gilgen, A. K.] ETH, Inst Agr Sci, CH-8092 Zurich, Switzerland. [Gilgen, A. K.] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland. [Gilgen, A. K.] Univ Bern, Oeschger Ctr Climate Change Res, CH-3013 Bern, Switzerland. [Dukes, J. S.; Hoeppner, S. S.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Dukes, J. S.] Univ Massachusetts, Dept Biol, Boston, MA 02125 USA. [Dukes, J. S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Estiarte, M.; Ogaya, R.; Sardans, J.] CREAF, Cerdanyola Del Valles 08193, Spain. [Estiarte, M.] Global Ecol Unit CREAF CEAB UAB, CSIC, Cerdanyola Del Valles 08193, Spain. [Gray, S. B.; Leakey, A. D. B.; Ort, D. R.; Siebers, M.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Gray, S. B.; Leakey, A. D. B.; Ort, D. R.; Siebers, M.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Guidolotti, G.] Univ Tuscia, Dept Innovat Biol Agrofood & Forest Syst, Viterbo, Italy. [Ort, D. R.] ARS, USDA, Urbana, IL 61801 USA. [Ostrogovic, M. Z.] Croatian Forest Res Inst, Jastrebarsko 10450, Croatia. [Rambal, S.] CEFE CNRS, F-34000 Montpellier, France. [Schmitt, M.] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [van der Linden, L.] Australian Water Qual Ctr, Adelaide, SA 5000, Australia. [van Straaten, O.] Univ Gottingen, Buesgen Inst, D-37077 Gottingen, Germany. [Granier, A.] INRA, UMR EEF 1137, F-54280 Champenoux, France. RP Vicca, S (reprint author), Univ Antwerp, Res Grp Plant & Vegetat Ecol, Dept Biol, Univ Pl 1, B-2610 Antwerp, Belgium. EM sara.vicca@ua.ac.be RI Dukes, Jeffrey/C-9765-2009; Leakey, Andrew/Q-9889-2016; Young, Kristina/M-3069-2014; Hoeppner, Susanne/C-4631-2015; Estiarte, Marc/G-2001-2016; Vicca, Sara/I-3637-2012; OI Dukes, Jeffrey/0000-0001-9482-7743; Leakey, Andrew/0000-0001-6251-024X; rambal, serge/0000-0001-5869-8382; Hoeppner, Susanne/0000-0003-2060-1666; Estiarte, Marc/0000-0003-1176-8480; Vicca, Sara/0000-0001-9812-5837; Sardans, Jordi/0000-0003-2478-0219 NR 18 TC 37 Z9 37 U1 10 U2 102 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD AUG PY 2012 VL 195 IS 3 BP 518 EP 522 DI 10.1111/j.1469-8137.2012.04224.x PG 5 WC Plant Sciences SC Plant Sciences GA 971CB UT WOS:000306179200006 PM 22734795 ER PT J AU Reitze, WT Sinkovec, C Huckell, BB AF Reitze, William T. Sinkovec, Christina Huckell, Bruce B. TI Folsom Technology and Toolstone Use at the Martin Site, North Central New Mexico SO PLAINS ANTHROPOLOGIST LA English DT Article DE Paleoindian; Folsom; Estancia Basin; hunter-gatherer mobility; raw material economies ID LITHIC TECHNOLOGY; MOBILITY; PLAINS; POINT; TEXAS; VIEW AB The Martin site is a Folsom camp in the Estancia Basin, Santa Fe County, New Mexico, investigated in 1955 and only briefly described in a 1967 dissertation. Reanalysis of the implement collection was conducted during 2002, and the debitage was described in detail in 2005. The principal goal of the research was to integrate this important assemblage into current models of Folsom lithic technological organization through study of artifact assemblage composition and identification of the raw materials used in the production of stone tools. The assemblage is dominated by large numbers of preforms and channel flakes that are predominantly (95 percent) Edwards chert from west central Texas sources. Despite the location of the Martin site far from known Edwards chert sources, only Folsom points were manufactured, thereby raising questions concerning the relationship between Folsom and Midland and the hypothesis that Midland point manufacture is a raw material conservation strategy employed by Folsom groups at sites distant from sources. We describe the assemblage and discuss the pattern of Folsom raw material consumption and technological organization. C1 [Reitze, William T.] Univ Arizona, Dept Anthropol, Tuscaloosa, AL USA. [Sinkovec, Christina] Cibola Natl Forest, USDA Forest Serv, Albuquerque, NM 87113 USA. [Huckell, Bruce B.] Univ New Mexico, Maxwell Museum Anthropol, Albuquerque, NM 87131 USA. [Huckell, Bruce B.] Univ New Mexico, Dept Anthropol, Albuquerque, NM 87131 USA. RP Reitze, WT (reprint author), Univ Arizona, Dept Anthropol, Tuscaloosa, AL USA. NR 69 TC 0 Z9 0 U1 1 U2 3 PU PLAINS ANTHROPOLOGICAL SOC PI LINCOLN PA 3201 SOUTH STREET, PO BOX 152, LINCOLN, NE 68506-3266 USA SN 0032-0447 J9 PLAINS ANTHROPOL JI Plains Anthropol. PD AUG PY 2012 VL 57 IS 223 BP 237 EP 259 PG 23 WC Anthropology SC Anthropology GA 229DU UT WOS:000325242800003 ER PT J AU Zhong, GY Yang, YZ AF Zhong, Gan-Yuan Yang, Yingzhen TI Characterization of grape Gibberellin Insensitive1 mutant alleles in transgenic Arabidopsis SO TRANSGENIC RESEARCH LA English DT Article DE GAI; Grapes; Plant architecture; Allelic variation ID GIBBERELLIN RESPONSES; GREEN-REVOLUTION; DELLA PROTEINS; FLORAL DEVELOPMENT; GENE ENCODES; ROOT-GROWTH; GAI GENE; RICE; MUTATION; RECEPTOR AB We generated 12 different mutations in the grape Gibberellin Insensitive1 (VvGAI1) sequences, transformed them into Arabidopsis under the control of 35S, Arabidopsis GAI or grape GAI1 promoter, and evaluated the impact of these mutant alleles on plant growth and development. These VvGAI1 sequence variants included some mimics of the known GAI-like mutant alleles discovered in grape, wheat, barley, corn, Brassica, and Arabidopsis. In general, plant height and related traits such as length of internodes and inflorescences were significantly reduced for most of the mutant alleles studied, regardless of which promoter was used. Interestingly, the numbers of rosette leaves and lateral branches were generally reduced when a 35S promoter was used to express the mutant alleles, but increased when an Arabidopsis or grape GAI promoter was used. Furthermore, the 35S plants often displayed curly and small leaves. In contrast, the leaves of the plants carrying mutant alleles controlled by a GAI promoter were of variable size, dark green and rarely curly. In addition, when certain VvGAI1 mutant alleles were under the control of the grape GAI1 promoter, the number of pods on inflorescences was significantly increased, but some of the pods produced few seeds due to partial sterility. On the basis of the systematic evaluation of various VvGAI1 mutant alleles in Arabidopsis, we concluded that the VvGAI1 mutant alleles mimicking the GAI or GAI-like mutant variants discovered in wheat, barley and Brassica could potentially be useful for the improvement of grapevine plant architecture. C1 [Zhong, Gan-Yuan; Yang, Yingzhen] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Zhong, GY (reprint author), ARS, USDA, Grape Genet Res Unit, 630W North St, Geneva, NY 14456 USA. EM ganyuan.zhong@ars.usda.gov NR 43 TC 2 Z9 2 U1 0 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 J9 TRANSGENIC RES JI Transgenic Res. PD AUG PY 2012 VL 21 IS 4 BP 725 EP 741 DI 10.1007/s11248-011-9565-z PG 17 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 972NJ UT WOS:000306283500004 PM 22038449 ER PT J AU Chen, F Yan, ZQ Liu, J Ji, J Chang, S Liu, D Qin, JP Ma, JY Bi, YZ Xie, QM AF Chen, Feng Yan, Zhuan-Qiang Liu, Jun Ji, Jun Chang, Shuang Liu, Di Qin, Jian-Ping Ma, Jing-Yun Bi, Ying-Zuo Xie, Qing-Mei TI Phylogenetic analysis of hemagglutinin genes of 40 H9N2 subtype avian influenza viruses isolated from poultry in China from 2010 to 2011 SO VIRUS GENES LA English DT Article DE Avian influenza virus; H9N2 subtype; Hemagglutinin gene; Phylogenetic analysis ID A-VIRUSES; RECEPTOR-BINDING; MAINLAND CHINA; HONG-KONG; EUROPE; ACID; ASIA; NEURAMINIDASE; TRANSMISSION; SPECIFICITY AB Avian influenza virus (H9N2) infection is a major problem of product performance in poultry worldwide. Vaccination is used to limit spread, but more knowledge is needed on the epidemiology of virus subtypes to improve vaccine design. In this study, 40 H9N2 subtype avian influenza viruses (AIVs) were isolated from vaccinated poultry flocks in China from 2010 to 2011. Hemagglutinin (HA) from different virus strains was sequenced and analyzed. We found that the HA genes of these strains shared nucleotide and deduced amino acid homologies that ranged from 90.1 to 92.9 and 91.4 to 95.0 %, respectively, when compared with vaccine strains. Phylogenetic analysis showed that the strains tested could be divided into two major groups. Group I consisted of 24 strains isolated mainly from Eastern and Central China. Group II consisted of 20 strains isolated from Southern China. The cleavage site within the HA protein contained two basic motifs, PSRSSRa dagger"GLF for group I, and PARSSRa dagger"GLF for group II. Additional potential glycosylation sites were found at amino acid position 295 in the HA1 of the isolates in group I, compared with isolates in group II and the vaccine strains. Furthermore, 38 out of the 40 isolates had a leucine residue at position 216 (aa 226 in H3), which was characteristic of human influenza virus-like receptor specificity. In the present study we found that geographical factors play a significant role in virus evolution, and emphasize the importance of continuing surveillance of H9N2 AIVs in chickens in China. C1 [Chen, Feng; Yan, Zhuan-Qiang; Ji, Jun; Liu, Di; Ma, Jing-Yun; Bi, Ying-Zuo; Xie, Qing-Mei] S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Liu, Jun; Qin, Jian-Ping] Guangdong Wens Foodstuffs Grp Co Ltd, Yunfu 527439, Peoples R China. [Chang, Shuang] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Xie, QM (reprint author), S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Science and Technology Planning Project of Guangdong Province China [2010B090301019] FX This research was supported by a grant from the Science and Technology Planning Project of Guangdong Province China (No. 2010B090301019). NR 31 TC 11 Z9 15 U1 0 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD AUG PY 2012 VL 45 IS 1 BP 69 EP 75 DI 10.1007/s11262-012-0742-9 PG 7 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 971OR UT WOS:000306214200009 PM 22476906 ER PT J AU Valles, SM Sabath, N AF Valles, Steven M. Sabath, Niv TI No evidence for translation of pog, a predicted overlapping gene of Solenopsis invicta virus 1 SO VIRUS GENES LA English DT Article DE Solenopsis invicta; RNA virus; Predicted overlapping gene; SINV-1 ID PCR AB An overlapping open reading frame (ORF) with a potential to encode a functional protein has been identified within the 3'-proximal ORF of Solenopsis invicta virus 1 (SINV-1) and three bee viruses. This ORF has been referred to as predicted overlapping gene (pog). Protein motif searches of POG revealed weak relationships precluding assignment of a potential function. Neither a transcript nor a protein encoded by the pog ORF has been detected. However, recently, a protein encoded by the corresponding +1 overlapping ORF (termed ORFx) in the Israeli acute paralysis virus (IAPV) was demonstrated by recombinant means as well as in IAPV-infected honey bees. The objective of our study was to attempt to provide empirical evidence for the presence of a pog-derived protein from SINV-1-infected fire ants. A number of different laboratory and field SINV-1-infected Solenopsis invicta preparations were examined by western blotting for the presence of a POG protein sequence. In every case, these preparations failed to yield any detectable bands when probed with a polyclonal antibody preparation raised to a portion of the pog predicted protein sequence. Although impossible to prove a negative result, proper controls used in these studies suggested that the pog ORF is not translated into a functional protein in SINV-1. C1 [Valles, Steven M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Sabath, Niv] Univ Zurich, Inst Evolutionary Biol & Environm Studies, CH-8057 Zurich, Switzerland. [Sabath, Niv] Swiss Inst Bioinformat, Basel, Switzerland. RP Valles, SM (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 18 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD AUG PY 2012 VL 45 IS 1 BP 84 EP 89 DI 10.1007/s11262-012-0746-5 PG 6 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 971OR UT WOS:000306214200011 PM 22528643 ER PT J AU Li, F Xu, DL Abad, J Li, RH AF Li, Fan Xu, Donglin Abad, Jorge Li, Ruhui TI Phylogenetic relationships of closely related potyviruses infecting sweet potato determined by genomic characterization of Sweet potato virus G and Sweet potato virus 2 SO VIRUS GENES LA English DT Article DE Sweet potato virus G; Sweet potato virus 2; Potyvirus; Genomic sequence; PISPO; Phylogeny ID FEATHERY-MOTTLE-VIRUS; COAT PROTEIN; MOLECULAR VARIABILITY; FAMILY POTYVIRIDAE; NONCODING REGION; GENUS POTYVIRUS; DISEASE SPVD; RNA; RECOMBINATION; STRAINS AB Complete nucleotide sequences of Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2) were determined to be 10,800 and 10,731 nucleotides, respectively, excluding the 3'-poly(A) tail. Their genomic organizations are typical of potyviruses, encoding a polyprotein which is likely cleaved into 10 mature proteins by three viral proteinases. Conserved motifs of orthologous proteins of viruses in the genus Potyvirus are found in corresponding positions of both viruses. Pairwise comparisons of individual protein sequences of the two viruses with those of 78 other potyviruses show that P1 protein and coat protein (CP) of both viruses are significantly large, with the SPVG CP as the largest among the all the known species of the genus Potyvirus. The extended N-terminal region of the P1 protein is conserved in the potyviruses and ipomovirus infecting sweet potato. A novel ORF, PISPO, is identified within the P1 region of SPVG, SPV2, Sweet potato feathery mottle virus (SPFMV), and Sweet potato virus C (SPVC). The C-terminal half of CP is highly conserved among SPFMV, SPVC, SPVG, SPV2, and Sweet potato virus-Zimbabwe. Phylogenetic analysis based on the deduced CP amino acid sequences supports the view that these five viruses are grouped together in a SPFMV lineage. The analysis also reveals that Sweet potato virus Y and Ipomoea vein mosaic virus are grouped with SPV2 as one species, and these two viruses should be consolidated with SPV2. C1 [Li, Fan; Xu, Donglin; Li, Ruhui] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Abad, Jorge] USDA APHIS PPQ PHP, Plant Germplasm Quarantine Program, Beltsville, MD 20705 USA. RP Li, RH (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. EM Ruhui.Li@ars.usda.gov OI Xu, Dongin/0000-0002-5719-2950 NR 40 TC 12 Z9 12 U1 0 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD AUG PY 2012 VL 45 IS 1 BP 118 EP 125 DI 10.1007/s11262-012-0749-2 PG 8 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 971OR UT WOS:000306214200016 PM 22562225 ER PT J AU Biswas, KK Tarafdar, A Diwedi, S Lee, RF AF Biswas, K. K. Tarafdar, A. Diwedi, S. Lee, R. F. TI Distribution, genetic diversity and recombination analysis of Citrus tristeza virus of India SO VIRUS GENES LA English DT Article DE CTV; Genetic diversity; Variant; Distribution; India ID VARIABILITY; SEQUENCE; ISOLATE; REGION; RNA AB Citrus tristeza virus (CTV) isolates representing all the citrus-growing geographical zones of India were analyzed for nucleotide sequence of the 5'ORF1a fragments of the partial LProI domain and for the coat protein (CP) gene. The nucleotide sequences were compared with previously reported Indian and CTV genotypes from GenBank. The Indian isolates had 80-99 % sequence identity for the 5'ORF1a and 89-99 % identity for the CP genes. In phylogenetic tree analysis, all the Indian and previously reported isolates segregated into eight clades or groups for the 5'ORF1a region. Indian CTV isolates were clustered in all the clades, four of which, D13, K5, BAN-1, and B165, consisted of only Indian isolates. Phylogenetic tree analysis of the CP genes resulted in seven clades. Indian CTV isolates clustered in six of them, and clades I and VI consisted of only Indian isolates. In the phylogenetic tree the Indian CTV isolates clustered in different groups regardless their geographical origin. Diversities in CTV isolates within individual citrus farms were highlighted. Because incongruent phylogenetic relationships were observed for both of the genomic regions, 5'ORF1a and CP gene, recombination analysis was performed using program RDP3. This analysis detected potential recombination events among the CTV isolates which involved exchange of sequences between divergent CTV variants. The SplitsTree analysis showed evidence of phylogenetic conflicts in evolutionary relationships among CTV isolates. C1 [Biswas, K. K.; Tarafdar, A.; Diwedi, S.] Indian Agr Res Inst, Adv Ctr Plant Virol, Div Plant Pathol, New Delhi 110012, India. [Lee, R. F.] USDA ARS, NCGRCD, Riverside, CA 92507 USA. RP Biswas, KK (reprint author), Indian Agr Res Inst, Adv Ctr Plant Virol, Div Plant Pathol, New Delhi 110012, India. EM kkbiswas@mailcity.com FU Department of Biotechnology, Govt. of India FX This work was supported by Department of Biotechnology, Govt. of India. The authors are grateful to K. L. Manjunath, USDA-ARS, NCGRCD, Riverside, CA 90507 for synthesis of primers for amplification of CTV sequences. We also thank R. K. Jain, HOD, Plant Pathology; Prof. Anupam Varma, National Professor, Y. S. Ahlawat and V. G. Malathi, In-charge, Plant Virology Unit; Director, Indian Agricultural Research Institute, New Delhi for providing facilities and their valuable suggestion. We are thankful to R. M. Gade, PDKV, Akola; K. B. Pun, IARI Regional Station, Kalimpong; V. M. Chavan, IARI Regional Station, Pune; for valuable support with surveys. NR 35 TC 11 Z9 13 U1 1 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD AUG PY 2012 VL 45 IS 1 BP 139 EP 148 DI 10.1007/s11262-012-0748-3 PG 10 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 971OR UT WOS:000306214200018 PM 22562224 ER PT J AU Jay-Russell, MT Bates, A Harden, L Miller, WG Mandrell, RE AF Jay-Russell, M. T. Bates, A. Harden, L. Miller, W. G. Mandrell, R. E. TI Isolation of Campylobacter from Feral Swine (Sus scrofa) on the Ranch Associated with the 2006 Escherichia coli O157:H7 Spinach Outbreak Investigation in California SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Campylobacter; Escherichia coli O157; MLST; PorA; matrix-assisted laser desorption ionization mass spectrometry; feral; swine; wild animals ID PIG-POPULATIONS; C-SPUTORUM; WILD PIG; JEJUNI; UPSALIENSIS; CATTLE; LANIENAE; SEQUENCE; LARI; TRANSMISSION AB We report the isolation of Campylobacter species from the same population of feral swine that was investigated in San Benito County, California, during the 2006 spinach-related Escherichia coli O157:H7 outbreak. This is the first survey of Campylobacter in a free-ranging feral swine population in the United States. Campylobacter species were cultured from buccal and rectal-anal swabs, colonic faeces and tonsils using a combination of selective enrichment and antibiotic-free membrane filtration methods. Matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF-MS, Bruker Daltonics, Inc., Billerica, MA, USA) was used to identify species followed by confirmatory multiplex PCR or 16S rRNA sequencing. Genetic relatedness of Campylobacter jejuni and Campylobacter coli strains was determined by multilocus sequence typing (MLST) and porA allele sequencing. Altogether, 12 (40%) of 30 feral swine gastrointestinal and oral cavity specimens were positive, and six species were isolated: Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalsis, Campylobacter jejuni, Campylobacter lanienae and Campylobacter sputorum. Campylobacter jejuni subtypes were closely related to MLST sequence type 21 (ST-21) and had identical porA sequences. Campylobacter coli subtypes were unrelated to isolates in the pubMLST/porA database. This feral swine population lived in close association with a grassfed beef cattle herd adjacent to spinach and other leafy green row crop fields. The findings underscore the importance of protecting raw vegetable crops from faecal contamination by wild or feral animals. The study also illustrates a potential risk of Campylobacter exposure for hunters during handling and processing of wild swine meat. C1 [Jay-Russell, M. T.] Univ Calif Davis, Western Inst Food Safety & Secur, Davis, CA 95616 USA. [Bates, A.; Harden, L.; Miller, W. G.; Mandrell, R. E.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. RP Jay-Russell, MT (reprint author), Univ Calif Davis, Western Inst Food Safety & Secur, 1 Shields Ave, Davis, CA 95616 USA. EM mjay@ucdavis.edu FU USDA ARS CRIS [5325-42000-045-00D] FX We are very grateful for technical assistance provided by Gerald Wiscomb, USDA Wildlife Services; Rosa Manalac, the Western Institute for Food Safety and Security, University of California Davis; and Feli Bautista at the USDA ARS Western Regional Research Center. This work was supported by funds from USDA ARS CRIS project #5325-42000-045-00D. NR 30 TC 6 Z9 7 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD AUG PY 2012 VL 59 IS 5 BP 314 EP 319 DI 10.1111/j.1863-2378.2012.01465.x PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA 972VK UT WOS:000306309000002 PM 22405465 ER PT J AU Cernicchiaro, N Pearl, DL McEwen, SA Harpster, L Homan, HJ Linz, GM LeJeune, JT AF Cernicchiaro, N. Pearl, D. L. McEwen, S. A. Harpster, L. Homan, H. J. Linz, G. M. LeJeune, J. T. TI Association of Wild Bird Density and Farm Management Factors with the Prevalence of E. coli O157 in Dairy Herds in Ohio (2007-2009) SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE E. coli O157; Sturnus vulgaris; starlings; birds; cattle; epidemiology ID TOXIGENIC ESCHERICHIA-COLI; CATTLE; RISK; CAMPYLOBACTER; TRANSMISSION; LISTERIA; FEEDLOT; H7 AB Our objective was to determine the role that European starlings (Sturnus vulgaris) play in the epidemiology of Escherichia coli O157:H7 in dairy cattle. We visited 150 dairy farms in Ohio twice during summer and fall months from 2007 to 2009. Fresh faecal pats from 30 lactating cows were collected during each visit. Information on farm management and environmental variables was gathered through a questionnaire administered to the farm owner. The number of starlings observed on the farm was also recorded. Approximately 1% of dairy cattle and 24% of farms were positive for E. coli O157. Risk factors associated with the presence of E. coli O157 in faecal pats included contact between adult cattle and calves, types or number of ventilation and manure management systems and number of birds per milking cow. C1 [Cernicchiaro, N.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Pearl, D. L.; McEwen, S. A.] Univ Guelph, Ontario Vet Coll, Dept Populat Med, Guelph, ON N1G 2W1, Canada. [Harpster, L.; LeJeune, J. T.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim & Hlth Res Program, Wooster, OH 44691 USA. [Homan, H. J.; Linz, G. M.] USDA, Natl Wildlife Res Ctr, Great Plains Field Stn, Bismarck, ND USA. RP Cernicchiaro, N (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Mosier Hall J118,1800 Denison Ave, Manhattan, KS 66506 USA. EM ncernic@vet.k-state.edu RI LeJeune, Jeff/B-3961-2012 FU United States Department of Agriculture through their National Research Initiative (USDA-NRI) [2006-01227]; Canada Foundation for Innovation; Ontario Ministry of Research and Innovation FX The original study was funded by the United States Department of Agriculture through their National Research Initiative (USDA-NRI), Epidemiological Approaches to Food Safety Grant # 2006-01227. The computational infrastructure for this research was obtained with the support of the Canada Foundation for Innovation and the Ontario Ministry of Research and Innovation through a grant to D.L. Pearl. NR 26 TC 15 Z9 16 U1 2 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD AUG PY 2012 VL 59 IS 5 BP 320 EP 329 DI 10.1111/j.1863-2378.2012.01457.x PG 10 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA 972VK UT WOS:000306309000003 PM 22333436 ER PT J AU Loharikar, A Briere, E Schwensohn, C Weninger, S Wagendorf, J Scheftel, J Garvey, A Warren, K Villamil, E Rudroff, JA Kurkjian, K Levine, S Colby, K Morrison, B May, A Anderson, S Daly, E Marsden-Haug, N Erdman, MM Gomez, T Rhorer, A Castleman, J Adams, JK Theobald, L Lafon, P Trees, E Mitchell, J Sotir, MJ Behravesh, CB AF Loharikar, A. Briere, E. Schwensohn, C. Weninger, S. Wagendorf, J. Scheftel, J. Garvey, A. Warren, K. Villamil, E. Rudroff, J. A. Kurkjian, K. Levine, S. Colby, K. Morrison, B. May, A. Anderson, S. Daly, E. Marsden-Haug, N. Erdman, M. M. Gomez, T. Rhorer, A. Castleman, J. Adams, J. K. Theobald, L. Lafon, P. Trees, E. Mitchell, J. Sotir, M. J. Behravesh, C. B. TI Four Multistate Outbreaks of Human Salmonella Infections Associated with Live Poultry Contact, United States, 2009 SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Salmonella; poultry; chicks; mail-order hatchery; zoonoses ID TYPHIMURIUM; PULSENET AB Outbreaks of human salmonellosis associated with live poultry contact have been reported since 1955. Multiple Salmonella serotypes have been associated with these outbreaks, and specific outbreak strains have been repeatedly linked to single hatcheries over multiple years. During 2009, four multistate outbreaks of human Salmonella infections associated with direct and indirect exposure to live poultry purchased from mail-order hatcheries and agricultural feed stores were identified, resulting in 165 culture-confirmed cases in 30 states. This report describes the epidemiologic, environmental and laboratory investigations conducted by state and local health departments, state departments of agriculture, the U.S. Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), National Poultry Improvement Plan (NPIP) and National Veterinary Services Laboratories (NVSL), and the Centers for Disease Control and Prevention (CDC). Case-patients were identified through PulseNet, the national molecular subtyping network for foodborne disease surveillance, and interviewed using the CDC standard live poultry contact questionnaire that asks about poultry-related exposures during the 7 days before illness onset. These outbreaks highlight the need to focus efforts on strategies to decrease and prevent human illness associated with live poultry contact through comprehensive interventions at the mail-order hatchery, agricultural feed store and consumer levels. Additional consumer education and interventions at mail-order hatcheries and venues where live poultry are sold, including agricultural feed stores, are necessary to prevent transmission of Salmonella from poultry to humans. C1 [Loharikar, A.; Briere, E.] Ctr Dis Control & Prevent, Epidem Intelligence Serv, Atlanta, GA 30333 USA. [Schwensohn, C.; Castleman, J.; Adams, J. K.; Theobald, L.; Lafon, P.; Trees, E.; Mitchell, J.; Sotir, M. J.; Behravesh, C. B.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30333 USA. [Weninger, S.; Wagendorf, J.] N Dakota Dept Hlth, Bismarck, ND USA. [Scheftel, J.] Minnesota Dept Hlth, St Paul, MN USA. [Garvey, A.] Iowa Dept Hlth, Des Moines, IA USA. [Warren, K.] Penn Dept Hlth, Wilkes Barre, PA USA. [Villamil, E.] New York State Dept Hlth, Albany, NY USA. [Rudroff, J. A.] Missouri Dept Hlth & Senior Serv, Jefferson City, MO USA. [Kurkjian, K.; Levine, S.] Virginia Dept Hlth, Richmond, VA USA. [Colby, K.] Univ So Maine, Maine Dept Hlth & Human Serv, Augusta, ME USA. [Morrison, B.] Maine Dept Agr, Augusta, ME USA. [May, A.] Indiana State Dept Hlth, Indianapolis, IN 46202 USA. [Anderson, S.] Kansas Dept Hlth & Environm, Topeka, KS USA. [Daly, E.] New Hampshire Dept Hlth & Human Serv, Concord, NH 03301 USA. [Marsden-Haug, N.] Washington State Dept Hlth, Shoreline, WA USA. [Erdman, M. M.] Natl Vet Serv Lab, Ames, IA 50010 USA. [Gomez, T.] Ctr Dis Control & Prevent, USDA, APHIS, Vet Serv Liason CDC, Atlanta, GA 30333 USA. [Rhorer, A.] USDA Natl Poultry Improvement Plan, Conyers, GA USA. RP Loharikar, A (reprint author), Ctr Dis Control & Prevent, Epidem Intelligence Serv, 1600 Clifton Rd,MS A-38, Atlanta, GA 30333 USA. EM aloharikar@cdc.gov NR 16 TC 10 Z9 10 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD AUG PY 2012 VL 59 IS 5 BP 347 EP 354 DI 10.1111/j.1863-2378.2012.01461.x PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA 972VK UT WOS:000306309000006 PM 22372941 ER PT J AU Tscharntke, T Tylianakis, JM Rand, TA Didham, RK Fahrig, L Batary, P Bengtsson, J Clough, Y Crist, TO Dormann, CF Ewers, RM Frund, J Holt, RD Holzschuh, A Klein, AM Kleijn, D Kremen, C Landis, DA Laurance, W Lindenmayer, D Scherber, C Sodhi, N Steffan-Dewenter, I Thies, C van der Putten, WH Westphal, C AF Tscharntke, Teja Tylianakis, Jason M. Rand, Tatyana A. Didham, Raphael K. Fahrig, Lenore Peter Batary Bengtsson, Janne Clough, Yann Crist, Thomas O. Dormann, Carsten F. Ewers, Robert M. Fruend, Jochen Holt, Robert D. Holzschuh, Andrea Klein, Alexandra M. Kleijn, David Kremen, Claire Landis, Doug A. Laurance, William Lindenmayer, David Scherber, Christoph Sodhi, Navjot Steffan-Dewenter, Ingolf Thies, Carsten van der Putten, Wim H. Westphal, Catrin TI Landscape moderation of biodiversity patterns and processes - eight hypotheses SO BIOLOGICAL REVIEWS LA English DT Review DE beta diversity; belowground-aboveground patterns; conservation management; ecosystem functioning and services; functional traits; insurance hypothesis; landscape composition and configuration; multitrophic interactions; resilience and stability; spatial heterogeneity ID DIFFERENT SPATIAL SCALES; AGRI-ENVIRONMENT SCHEMES; LAND-USE INTENSITY; EXPERIMENTALLY FRAGMENTED LANDSCAPE; SPECIES-AREA RELATIONSHIPS; TROPICAL HABITAT GRADIENT; SOIL DECOMPOSER COMMUNITY; BIOLOGICAL-CONTROL AGENTS; NATURAL ENEMY DIVERSITY; FOOD-WEB STRUCTURE AB Understanding how landscape characteristics affect biodiversity patterns and ecological processes at local and landscape scales is critical for mitigating effects of global environmental change. In this review, we use knowledge gained from human-modified landscapes to suggest eight hypotheses, which we hope will encourage more systematic research on the role of landscape composition and configuration in determining the structure of ecological communities, ecosystem functioning and services. We organize the eight hypotheses under four overarching themes. Section A: landscape moderation of biodiversity patterns' includes (1) the landscape species pool hypothesisthe size of the landscape-wide species pool moderates local (alpha) biodiversity, and (2) the dominance of beta diversity hypothesislandscape-moderated dissimilarity of local communities determines landscape-wide biodiversity and overrides negative local effects of habitat fragmentation on biodiversity. Section B: landscape moderation of population dynamics' includes (3) the cross-habitat spillover hypothesislandscape-moderated spillover of energy, resources and organisms across habitats, including between managed and natural ecosystems, influences landscape-wide community structure and associated processes and (4) the landscape-moderated concentration and dilution hypothesisspatial and temporal changes in landscape composition can cause transient concentration or dilution of populations with functional consequences. Section C: landscape moderation of functional trait selection includes (5) the landscape-moderated functional trait selection hypothesislandscape moderation of species trait selection shapes the functional role and trajectory of community assembly, and (6) the landscape-moderated insurance hypothesislandscape complexity provides spatial and temporal insurance, i.e. high resilience and stability of ecological processes in changing environments. Section D: landscape constraints on conservation management' includes (7) the intermediate landscape-complexity hypothesislandscape-moderated effectiveness of local conservation management is highest in structurally simple, rather than in cleared (i.e. extremely simplified) or in complex landscapes, and (8) the landscape-moderated biodiversity versus ecosystem service management hypothesislandscape-moderated biodiversity conservation to optimize functional diversity and related ecosystem services will not protect endangered species. Shifting our research focus from local to landscape-moderated effects on biodiversity will be critical to developing solutions for future biodiversity and ecosystem service management. C1 [Tscharntke, Teja; Peter Batary; Clough, Yann; Fruend, Jochen; Scherber, Christoph; Thies, Carsten; Westphal, Catrin] Univ Gottingen, Dept Crop Sci, D-37077 Gottingen, Germany. [Tylianakis, Jason M.; Didham, Raphael K.] Univ Canterbury, Sch Biol Sci, Christchurch 8140, New Zealand. [Rand, Tatyana A.] ARS, USDA, No Plains Agr Res Lab, Sidney, MT 59270 USA. [Didham, Raphael K.] Univ Western Australia, Sch Anim Biol, Crawley, WA 6009, Australia. [Didham, Raphael K.] CSIRO Entomol, Ctr Environm & Life Sci, Floreat, WA 6014, Australia. [Fahrig, Lenore] Carleton Univ, Dept Biol, Geomat & Landscape Ecol Lab, Ottawa, ON K1S 5B6, Canada. [Peter Batary] MTA ELTE MTM Ecol Res Grp, H-1083 Budapest, Hungary. [Bengtsson, Janne] Swedish Univ Agr Sci, Dept Ecol, S-75007 Uppsala, Sweden. [Crist, Thomas O.] Miami Univ, Inst Environm & Sustainabil, Oxford, OH 45056 USA. [Crist, Thomas O.] Miami Univ, Dept Zool, Oxford, OH 45056 USA. [Dormann, Carsten F.] UFZ Helmholtz Ctr Environm Res, Dept Computat Landscape Ecol, D-04318 Leipzig, Germany. [Ewers, Robert M.] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, Ascot SL5 7PY, Berks, England. [Holt, Robert D.] Univ Florida, Gainesville, FL 32611 USA. [Holzschuh, Andrea; Steffan-Dewenter, Ingolf] Univ Wurzburg, Bioctr, Dept Anim Ecol & Trop Biol, D-97074 Wurzburg, Germany. [Klein, Alexandra M.] Univ Luneburg, Inst Ecol & Environm Chem, Fac 3, D-21335 Luneburg, Germany. [Kleijn, David] Alterra, Ctr Ecosyst Studies, NL-6700 AA Wageningen, Netherlands. [Kremen, Claire] Univ Calif Berkeley, Berkeley, CA 94720 USA. [Landis, Doug A.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Landis, Doug A.] Michigan State Univ, Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [Laurance, William] James Cook Univ, Ctr Trop Environm & Sustainabil Sci TESS, Cairns, Qld 4870, Australia. [Laurance, William] James Cook Univ, Sch Marine & Trop Biol, Cairns, Qld 4870, Australia. [Lindenmayer, David] Australian Natl Univ, Fenner Sch Environm & Soc, Canberra, ACT 0200, Australia. [Sodhi, Navjot] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore. [van der Putten, Wim H.] Netherlands Inst Ecol, NL-6700 AB Wageningen, Netherlands. [van der Putten, Wim H.] Univ Wageningen & Res Ctr, Nematol Lab, NL-6700 ES Wageningen, Netherlands. RP Tscharntke, T (reprint author), Univ Gottingen, Dept Crop Sci, Grisebachstr 6, D-37077 Gottingen, Germany. EM ttschar@gwdg.de RI Scherber, Christoph/B-6611-2009; Didham, Raphael/B-5953-2011; James Cook University, TESS/B-8171-2012; van der Putten, Wim/C-3707-2011; Batary, Peter/A-2349-2008; Clough, Yann/B-9739-2014; Tylianakis, Jason/B-6634-2011; Tscharntke, Teja/N-5123-2014; Research ID, CTBCC /O-3564-2014; Westphal, Catrin/F-9560-2015; OI Frund, Jochen/0000-0002-7079-3478; Steffan-Dewenter, Ingolf/0000-0003-1359-3944; van der Putten, Wim/0000-0002-9341-4442; Batary, Peter/0000-0002-1017-6996; Clough, Yann/0000-0002-2901-7602; Tylianakis, Jason/0000-0001-7402-5620; Westphal, Catrin/0000-0002-2615-1339; Scherber, Christoph/0000-0001-7924-8911; Kleijn, David/0000-0003-2500-7164; Dormann, Carsten/0000-0002-9835-1794 FU German Ministry of Research and Education (BMBF); German Research Foundation (DFG); Helmholtz Association [VH-NG-247]; Marsden Fund of New Zealand [UOC-0802]; Natural Sciences and Engineering Research Council of Canada; Canada Foundation for Innovation; University of Florida Foundation; EU [226852, 244090-STEP-CP-FP]; US National Science Foundation; US Department of Energy Great Lakes Bioenergy Research Center FX We note with sadness the passing of our friend and colleague Navjot Sodhi, and hope that he would have approved of the revised version of this manuscript. Author sequence follows the "sequence-determines-credit" (from T. T. to L. F.) and the "equal-contribution'' norm (from P. B. to C. W.) (see Tscharntke et al., 2007b). Two anonymous reviewers provided very thoughtful advice. Financial support for T. T., Y.C., A. H., A. M. K., C. S., I. S. D., C. T., J.F. and C. W. came from the German Ministry of Research and Education (BMBF) and the German Research Foundation (DFG), for T. T., P. B. and C. F. D. from the Helmholtz Association (VH-NG-247, Research Group BESS "Biological Ecosystem Services''), for J.M.T. and R. K. D. from the Marsden Fund of New Zealand (UOC-0802), for L. F. from the Natural Sciences and Engineering Research Council of Canada and the Canada Foundation for Innovation, to R. D. H. from the University of Florida Foundation, for I. S. D. and A. H. from the EU FP7 projects "SCALES" (Contract No. 226852) and "STEP" (Contract No. 244090-STEP-CP-FP), and for D. A. L. from the US National Science Foundation Long-Term Ecological Research Program and the US Department of Energy Great Lakes Bioenergy Research Center. P. B. was a Bolyai Research Fellow of the Hungarian Academy of Sciences. Susanne Schiele is gratefully acknowledged for her help. NR 329 TC 335 Z9 352 U1 93 U2 919 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-7931 EI 1469-185X J9 BIOL REV JI Biol. Rev. PD AUG PY 2012 VL 87 IS 3 BP 661 EP 685 DI 10.1111/j.1469-185X.2011.00216.x PG 25 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 968OL UT WOS:000305992300010 PM 22272640 ER PT J AU Epanchin-Niell, RS Haight, RG Berec, L Kean, JM Liebhold, AM AF Epanchin-Niell, Rebecca S. Haight, Robert G. Berec, Ludek Kean, John M. Liebhold, Andrew M. TI Optimal surveillance and eradication of invasive species in heterogeneous landscapes SO ECOLOGY LETTERS LA English DT Article DE Biological invasions; cost efficient; detection; eradication; gypsy moth; Lymantria dispar; monitoring; pest management; resource allocation; risk management ID UNITED-STATES; GYPSY-MOTH; MANAGEMENT; DYNAMICS; MODEL AB Cost-effective surveillance strategies are needed for efficient responses to biological invasions and must account for the trade-offs between surveillance effort and management costs. Less surveillance may allow greater population growth and spread prior to detection, thereby increasing the costs of damages and control. In addition, surveillance strategies are usually applied in environments under continual invasion pressure where the number, size and location of established populations are unknown prior to detection. We develop a novel modeling framework that accounts for these features of the decision and invasion environment and determines the long term sampling effort that minimises the total expected costs of new invasions. The optimal solution depends on population establishment and growth rates, sample sensitivity, and sample, eradication, and damage costs. We demonstrate how to optimise surveillance systems under budgetary constraints and find that accounting for spatial heterogeneity in sampling costs and establishment rates can greatly reduce management costs. C1 [Epanchin-Niell, Rebecca S.] Resources Future Inc, Washington, DC 20036 USA. [Haight, Robert G.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Berec, Ludek] Acad Sci Czech Republic, Inst Entomol, Ctr Biol, CR-37005 Ceske Budejovice, Czech Republic. [Kean, John M.] AgRes Lincoln, Christchurch 8140, New Zealand. [Liebhold, Andrew M.] US Forest Serv, No Res Stn, Morgantown, WV 26505 USA. RP Epanchin-Niell, RS (reprint author), Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. EM epanchin-niell@rff.org RI Liebhold, Andrew/C-1423-2008; Berec, Ludek/G-7253-2014 OI Liebhold, Andrew/0000-0001-7427-6534; Berec, Ludek/0000-0002-2419-3324 NR 30 TC 37 Z9 39 U1 6 U2 70 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X J9 ECOL LETT JI Ecol. Lett. PD AUG PY 2012 VL 15 IS 8 BP 803 EP 812 DI 10.1111/j.1461-0248.2012.01800.x PG 10 WC Ecology SC Environmental Sciences & Ecology GA 967YB UT WOS:000305943000005 PM 22642613 ER PT J AU Slosky, LM Hoffmann, EJ Hagler, JR AF Slosky, Lauren M. Hoffmann, Eric J. Hagler, James R. TI A comparative study of the retention and lethality of the first and second generation arthropod protein markers SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE arthropod dispersal; protein marking; enzyme-linked immunosorbent assay; Hippodamia convergens; mark-capture; mark-release-recapture; Coleoptera; Coccinellidae; convergent lady beetle ID SUBTERRANEAN TERMITE ISOPTERA; IMMUNOMARKING TECHNIQUE; MARKING TECHNIQUE; INSECT PREDATORS; DISPERSAL; MOVEMENT; PARASITOIDS; RHINOTERMITIDAE; POPULATION AB A greenhouse study was conducted that compared the protein mark retention time of a well-established rabbit IgG protein detection protocol with those of three newer, less expensive protein detection protocols designed to detect casein in bovine milk, egg albumin in chicken egg whites, and soy trypsin in soy milk, respectively. Adult convergent lady beetles, Hippodamia convergens Guerin-Meneville (Coleoptera: Coccinellidae), were topically marked with either 2.0 ml of a 5.0 mg ml(-1) rabbit IgG solution or 2.0 ml of pure bovine milk, chicken egg whites, or soy milk solutions. The variously marked cohorts of beetles were then released into cages that contained a single cotton plant. In turn, beetles were collected every other day for 26 days after marking and assayed for the presence of the protein marks by either a sandwich anti-rabbit IgG enzyme-linked immunosorbent assay (ELISA) or an indirect anti-casein, anti-egg albumin, or anti-soy trypsin ELISA to detect bovine casein, chicken egg albumin, or soy milk trypsin, respectively. Data indicate that the durability of the protein markers on the beetles decreases in the following order: egg whites > rabbit IgG > milk > soy milk. In addition, the mortality of H. convergens after receiving each protein mark treatment was assessed. Data revealed that there were no significant differences in mean mortality of beetles between any of the protein treatments. Overall mortality during the course of the 26-day study ranged from 30% for the soy-marked beetles to 50% for the water and rabbit IgG-marked beetles. C1 [Slosky, Lauren M.; Hoffmann, Eric J.; Hagler, James R.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Slosky, Lauren M.] Univ Arizona, Undergrad Biol Res Program, Tucson, AZ 85719 USA. RP Hagler, JR (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov FU University of Arizona's Undergraduate Biology Research Program (Howard Hughes Medical Institute); CSREES Risk Avoidance and Mitigation Program (RAMP) FX We thank Felisa Blackmer, Joel Gilley, Amanda Hagler, Scott Machtley, Chris McNeely, and Samantha Werts for their meticulous technical assistance. A special thanks to Dr. Bruce Mackey for providing expert statistical advice. Funding for the project was provided, in part, by the University of Arizona's Undergraduate Biology Research Program (Howard Hughes Medical Institute) and the CSREES Risk Avoidance and Mitigation Program (RAMP). Mention of proprietary or brand names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval to the exclusion of others that also may be suitable. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of source. NR 23 TC 9 Z9 9 U1 4 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD AUG PY 2012 VL 144 IS 2 BP 165 EP 171 DI 10.1111/j.1570-7458.2012.01274.x PG 7 WC Entomology SC Entomology GA 967XR UT WOS:000305942000003 ER PT J AU McKinley, DC Briggs, RD Bartuska, AM AF McKinley, Duncan C. Briggs, Russell D. Bartuska, Ann M. TI When peer-reviewed publications are not enough! Delivering science for natural resource management SO FOREST POLICY AND ECONOMICS LA English DT Article DE Forest management; Natural resource management; Citizen science; Participatory research; Public participation; Democracy ID PARTICIPATORY ACTION RESEARCH; CITIZEN-SCIENCE; ECOSYSTEM MANAGEMENT; POLICY-MAKING; CONSERVATION BIOLOGISTS; SCIENTIFIC-KNOWLEDGE; ENVIRONMENTAL-POLICY; DECISION-MAKING; TECHNOLOGY; SOCIETY AB Over the last century, natural resource management on forest lands has shifted from a singular focus on resource extraction to many foci, such as recreation, tourism, conservation, view-scapes, cultural and spiritual values, sustainability, and other values. As a result, the information needs of land managers must now include social and cultural values. In addition, the public's interest in having greater participation in land management decisions and in generating scientific knowledge has never been greater. The generation of scientific knowledge which is expressed primarily through conventional means - such as peer-reviewed publications targeting academics and technology transfer (e.g., patents, licenses, agreements) primarily for government and industry - does not always satisfy the needs of resource managers and public. In recent decades, there has been rapid growth of methods to help bridge this gap by better connecting new knowledge and knowledge generation with public needs. The U.S. Forest Service is making science delivery as important goal as science creation, including structural institutional changes at the interface among researchers, resource managers, and the public, allocating an appropriate portion of project funding specifically for delivery. The Forest Service is considering increasing its use of citizen science and participatory research - which brings resource managers, decision makers, and the public into the research process to varying extents - as part of the agency's science delivery efforts. Here we explore citizen science and participatory research as possible vehicles to augment existing science delivery efforts from the perspective of a federal land management agency. We found that these mechanisms facilitate public involvement in fundamentally different ways. Depending on the type of research and desired use of research outcomes, either citizen science or participatory research could enhance the use of science in some natural resource management discussions, possibly leading to supportable solutions. Published by Elsevier B.V. C1 [McKinley, Duncan C.] US Forest Serv, USDA, Washington, DC 20250 USA. [Briggs, Russell D.] SUNY Coll Environm Sci & Forestry ESF, Syracuse, NY 13210 USA. RP McKinley, DC (reprint author), US Forest Serv, USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. EM dcmckinley@fs.fed.us; rdbriggs@esf.edu; Ann.Bartuska@osec.usda.gov NR 111 TC 21 Z9 21 U1 10 U2 83 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 J9 FOREST POLICY ECON JI Forest Policy Econ. PD AUG PY 2012 VL 21 BP 1 EP 11 DI 10.1016/j.forpol.2012.03.007 PG 11 WC Forestry SC Forestry GA 967TR UT WOS:000305931200001 ER PT J AU Prestemon, JP Abt, KL Barbour, RJ AF Prestemon, Jeffrey P. Abt, Karen L. Barbour, R. James TI Quantifying the net economic benefits of mechanical wildfire hazard treatments on timberlands of the western United States SO FOREST POLICY AND ECONOMICS LA English DT Article DE Fuel; Timber; Products; Wildland-urban interface; Monte Carlo ID FOREST-FIRE MANAGEMENT; FUEL TREATMENTS; MARKET IMPACTS; PROGRAMS; BEHAVIOR; GROWTH; US AB Mechanical treatment of vegetation is done on public and private lands for many possible reasons, including enhancing wildlife habitat, increasing timber growth of residual stands, and improving resistance to damaging pests. Few studies, however, have focused on the circumstances under which mechanical wildfire hazard reduction treatments can yield positive net economic wildfire benefits for landowners and managers. This study describes an economic assessment tool built from a representative area sample frame inventory of hazardous and potentially treatable timberland in twelve western states of the U.S. Base case parameter assumptions about values at risk, timber product prices, stand re-growth following treatment and wildfire impacts enable an initial estimate of the amount of timberland with positive discounted expected net economic benefits under four policy scenarios. These assumptions are then varied in a Monte Carlo simulation to provide some bounds of uncertainty around base case levels. A policy that allowed optimal prescriptions and product sales and which incorporates wildfire costs and benefits would result in more than 25% of treated area with positive net benefits. This is reduced somewhat if wildfire reduction costs and benefits are not considered, and reduced again to 14% when large trees are excluded from product sales. A policy that prohibits sale of products from these treatments results in less than 1% of area with positive net benefits. Published by Elsevier B.V. C1 [Prestemon, Jeffrey P.; Abt, Karen L.] US Forest Serv, So Res Stn, USDA, Res Triangle Pk, NC 27709 USA. [Barbour, R. James] US Forest Serv, Pacific NW Res Stn, USDA, Portland, OR 97204 USA. RP Prestemon, JP (reprint author), US Forest Serv, So Res Stn, USDA, POB 12254, Res Triangle Pk, NC 27709 USA. EM jprestemon@fs.fed.us; kabt@fs.fed.us; jbarbour01@fs.fed.us FU Joint Fire Science Program [01-1-2-09] FX We thank Armando Gonzalez-Caban, Jeffrey D. Kline, Eric White, and Robert Rummer for helpful comments in an initial draft of this article. We also thank James Jeuck for his assistance with geographic information and mapping. Original funding for the initial phases of model development for this work was derived from Joint Fire Science Program Grant 01-1-2-09. NR 33 TC 5 Z9 5 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 J9 FOREST POLICY ECON JI Forest Policy Econ. PD AUG PY 2012 VL 21 BP 44 EP 53 DI 10.1016/j.forpol.2012.02.006 PG 10 WC Forestry SC Forestry GA 967TR UT WOS:000305931200005 ER EF