FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Klopfenstein, NB Hanna, JW Kim, M AF Klopfenstein, N. B. Hanna, J. W. Kim, M. TI Potential invasiveness of Armillaria solidipes, a tree-root-disease pathogen with a circumboreal distribution SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Klopfenstein, N. B.; Hanna, J. W.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID USA. [Kim, M.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S92 EP S92 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400539 ER PT J AU Klosterman, SJ Subbarao, KV Kang, S Veronese, P Gold, SE Thomma, BP Chen, Z Henrissat, B Lee, Y Park, J Garcia-Pedrajas, MD Barbara, DJ Anchieta, A de Jonge, R Santhanam, P Maruthachalam, K Atallah, Z Amyotte, SG Paz, Z Inderbitzin, P Hayes, RJ Heiman, DI Young, S Zeng, Q Engels, R Galagan, J Cuomo, C Dobinson, KF Ma, L AF Klosterman, S. J. Subbarao, K. V. Kang, S. Veronese, P. Gold, S. E. Thomma, B. P. Chen, Z. Henrissat, B. Lee, Y. Park, J. Garcia-Pedrajas, M. D. Barbara, D. J. Anchieta, A. de Jonge, R. Santhanam, P. Maruthachalam, K. Atallah, Z. Amyotte, S. G. Paz, Z. Inderbitzin, P. Hayes, R. J. Heiman, D. I. Young, S. Zeng, Q. Engels, R. Galagan, J. Cuomo, C. Dobinson, K. F. Ma, L. TI Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Klosterman, S. J.; Anchieta, A.; Hayes, R. J.] ARS, USDA, Salinas, CA USA. [Subbarao, K. V.; Maruthachalam, K.; Inderbitzin, P.] Univ Calif Davis, Davis, CA 95616 USA. [Kang, S.] Penn State Univ, University Pk, PA 16802 USA. [Veronese, P.] N Carolina State Univ, Raleigh, NC 27695 USA. [Gold, S. E.; Paz, Z.] Univ Georgia, Athens, GA 30602 USA. [Thomma, B. P.; de Jonge, R.; Santhanam, P.] Wageningen Univ, Wageningen, Netherlands. [Chen, Z.; Heiman, D. I.; Young, S.; Zeng, Q.; Engels, R.; Galagan, J.; Cuomo, C.; Ma, L.] Broad Inst, Cambridge, MA USA. [Henrissat, B.] Univ Aix Marseille, CNRS, Marseille, France. [Lee, Y.; Park, J.] Seoul Natl Univ, Seoul, South Korea. [Garcia-Pedrajas, M. D.] CSIC, Estac Expt La Mayora, Malaga, Spain. [Barbara, D. J.] Univ Warwick, Warwick, England. [Atallah, Z.] Univ Calif, Salinas, CA USA. [Amyotte, S. G.] Univ Western Ontario, London, ON, Canada. [Dobinson, K. F.] Agr & Agri Food Canada, London, ON, Canada. RI Henrissat, Bernard/J-2475-2012; Thomma, Bart/E-5959-2011 OI Thomma, Bart/0000-0003-4125-4181 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S208 EP S208 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401633 ER PT J AU Kluepfel, DA Aradhaya, MK Moersfelder, JW McClean, AE Hackett, WP Dull, AJ AF Kluepfel, D. A. Aradhaya, M. K. Moersfelder, J. W. McClean, A. E. Hackett, W. P. Dull, A. J. TI Evaluation of wild walnut Juglans spp. for resistance to crown gall disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Kluepfel, D. A.; Aradhaya, M. K.; Moersfelder, J. W.; McClean, A. E.; Dull, A. J.] Univ Calif Davis, ARS, USDA, Davis, CA 95616 USA. [Hackett, W. P.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S92 EP S92 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400540 ER PT J AU Kousik, CS AF Kousik, C. S. TI Sources of resistance to Phytophthora fruit rot in watermelon plant introductions SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Kousik, C. S.] ARS, US Vegetable Lab, USDA, Charleston, SC USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S94 EP S94 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400549 ER PT J AU Kunta, M Rascoe, J Palm, ME da Graca, JV Salas, B Satpute, A Setamou, M Snow, PBD Skaria, M AF Kunta, M. Rascoe, J. Palm, M. E. da Graca, J. V. Salas, B. Satpute, A. Setamou, M. Snow, P. B. de Sa Skaria, M. TI First report of Sweet orange scab in USA SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Kunta, M.; da Graca, J. V.; Satpute, A.; Setamou, M.; Skaria, M.] Texas A&M Univ Kingsville, Weslaco, TX USA. [Rascoe, J.; Palm, M. E.] USDA APHIS PPQ PHP RIPPS, Beltsville, MD USA. [Salas, B.] USDA APHIS PPQ CPHST PDDML, Mission, TX USA. [Snow, P. B. de Sa] USDA APHIS PPQ PHP RIPPS MDL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S95 EP S95 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400556 ER PT J AU Kuykendall, L Davenport, F Shao, JY Maroon-Lango, CJ AF Kuykendall, L. Davenport, F. Shao, J. Y. Maroon-Lango, C. J. TI PCR-based detection, by use of degenerate primers, of an EngA cellulase gene in Xanthomonas sacchari from asymptomatic sugarcane SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Kuykendall, L.; Davenport, F.] ARS, Natl Germplasm Resources Lab, BARC West, Natl Germplasm Resources Lab, Beltsville, MD USA. [Kuykendall, L.; Shao, J. Y.] ARS, Mol Plant Pathol Lab, USDA, BARC West, Beltsville, MD USA. [Maroon-Lango, C. J.] USDA APHIS PPQ PHP Plant Germplasm Quarantine Pro, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S96 EP S96 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400563 ER PT J AU Lacava, PT Gai, CS Miller, TA Hartung, JS Azevedo, J AF Lacava, P. T. Gai, C. S. Miller, T. A. Hartung, J. S. Azevedo, J. TI Strategies of biological and symbiotic control of citrus variegated chlorosis by endophytic bacteria SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lacava, P. T.] Fed Univ Alfenas UNIFAL MG, Alfenas, Brazil. [Gai, C. S.; Azevedo, J.] Univ Sao Paulo, Piracicaba, Brazil. [Miller, T. A.] Univ Calif Riverside, Riverside, CA 92521 USA. [Hartung, J. S.] ARS, USDA, Beltsville, MD USA. RI Miller, Thomas/F-7512-2012 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S96 EP S96 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400565 ER PT J AU Lakshman, DK Nguyen, TC Han, J Galvez, LC Mitra, A AF Lakshman, D. K. Nguyen, T. C. Han, J. Galvez, L. C. Mitra, A. TI Transgenic plants expressing antimicrobial lactoferrin protein are resistant to Rhizoctonia solani SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lakshman, D. K.] ARS, USDA, Beltsville, MD USA. [Nguyen, T. C.; Han, J.; Galvez, L. C.; Mitra, A.] Univ Nebraska, Lincoln, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S97 EP S97 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400568 ER PT J AU Larkin, RP Olanya, M He, Z Halloran, J Honeycutt, W AF Larkin, R. P. Olanya, M. He, Z. Halloran, J. Honeycutt, W. TI Use of disease-suppressive Brassica rotation crops in potato production: Overview of 10 years of field trials SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Olanya, M.; He, Z.; Halloran, J.; Honeycutt, W.] ARS, USDA, NEPSWL, Orono, ME USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S98 EP S98 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400576 ER PT J AU Lartey, RT Ghoshroy, K Caesar-TonThat, T Evans, RG Ghoshroy, S AF Lartey, R. T. Ghoshroy, K. Caesar-TonThat, T. Evans, R. G. Ghoshroy, S. TI Basis for inhibition of Pyrenophora teres by Laetisaria arvalis, a scanning and transmission electron microscopic study SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lartey, R. T.; Caesar-TonThat, T.; Evans, R. G.] ARS, USDA, Sidney, MT USA. [Ghoshroy, K.] Univ S Carolina Sumter, Div Sci Math & Engn, Sumter, SC USA. [Ghoshroy, S.] Univ S Carolina, Electron Microscopy Ctr, Columbia, SC 29208 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S98 EP S98 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400577 ER PT J AU Lartey, RT Caesar-TonThat, T Ghoshroy, K Evans, RG Caesar, AJ Hanson, S Sainju, UM AF Lartey, R. T. Caesar-TonThat, T. Ghoshroy, K. Evans, R. G. Caesar, A. J. Hanson, S. Sainju, U. M. TI Detection of Pyrenophora teres in conidia and barley seed by PCR, a technique for rapid diagnosis of infestation SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lartey, R. T.; Caesar-TonThat, T.; Evans, R. G.; Caesar, A. J.; Hanson, S.; Sainju, U. M.] ARS, USDA, Sidney, MT USA. [Ghoshroy, K.] Univ S Carolina Sumter, Div Sci Math & Engn, Sumter, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S98 EP S99 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400578 ER PT J AU Lee, M Rogers, EE Stenger, DC AF Lee, M. Rogers, E. E. Stenger, D. C. TI PemK toxin encoded by the Xylella fastidiosa IncP-1 plasmid pXF-RIV11 is a ribonuclease SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lee, M.; Rogers, E. E.; Stenger, D. C.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S99 EP S99 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400583 ER PT J AU Lee, S Mian, M Wang, H McHale, L Sneller, C Dorrance, A AF Lee, S. Mian, M. Wang, H. McHale, L. Sneller, C. Dorrance, A. TI Identification of quantitative trait loci conferring partial resistance to Phytophthora sojae in soybean PI 427106 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lee, S.; Sneller, C.] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH USA. [Mian, M.] ARS, Corn & Soybean Unit, USDA, Wooster, OH USA. [Wang, H.; Dorrance, A.] Ohio State Univ, Dept Plant Pathol, Wooster, OH USA. [McHale, L.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S100 EP S100 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045400585 ER PT J AU Li, F Xu, D Abad, J Li, R AF Li, F. Xu, D. Abad, J. Li, R. TI Phylogenetic relationships of closely related potyviruses infecting sweet potato determined by genomic characterization of Sweet potato virus 2 and Sweet potato virus G SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, F.; Xu, D.; Li, R.] ARS, USDA, Beltsville, MD USA. [Abad, J.] USDA, APHIS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S102 EP S102 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401010 ER PT J AU Li, F Zuo, R Xu, D Abad, J Li, R AF Li, F. Zuo, R. Xu, D. Abad, J. Li, R. TI Simultaneous detection and differentiation of four sweet potato potyviruses by one-step RT-PCR SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, F.; Xu, D.; Li, R.] ARS, USDA, Beltsville, MD USA. [Zuo, R.] Yunnan Agr Univ, Kunming, Peoples R China. [Abad, J.] USDA, APHIS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S102 EP S102 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401009 ER PT J AU Li, S Chen, P Rupe, JC Wrather, A Sciumbato, G Smith, J Nelson, R AF Li, S. Chen, P. Rupe, J. C. Wrather, A. Sciumbato, G. Smith, J. Nelson, R. TI Identification of soybean accessions with resistance to Phomopsis seed decay: Joint effort from USDA and University scientists SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, S.; Smith, J.] ARS, USDA, Crop Genet Res Unit CGRU, Stoneville, MS USA. [Chen, P.; Rupe, J. C.] Univ Arkansas, Fayetteville, AR 72701 USA. [Wrather, A.] Univ Missouri, Portageville, MO USA. [Sciumbato, G.] Univ Mississippi, Stoneville, MS USA. [Nelson, R.] ARS, USDA, Genet Res Unit, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S103 EP S103 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401014 ER PT J AU Li, S Jin, X Chen, J AF Li, S. Jin, X. Chen, J. TI Effects of venom alkaloids from red imported fire ants on bacterial canker of tomato in the greenhouse SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, S.] Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding, Hebei Province, Peoples R China. [Jin, X.; Chen, J.] ARS, USDA, MSA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S101 EP S101 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401005 ER PT J AU Li, Y Windham, M Trigiano, R Windham, A Spiers, J AF Li, Y. Windham, M. Trigiano, R. Windham, A. Spiers, J. TI Anthracnose of Miscanthus sinensis caused by Colletotrichum graminicola SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, Y.] Connecticut Agr Expt Stn, New Haven, CT 06504 USA. [Spiers, J.] USDA ARS, Poplarville, MS USA. [Windham, M.; Trigiano, R.; Windham, A.] Univ Tennessee, Knoxville, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S260 EP S261 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401908 ER PT J AU Liangsheng, X Chen, W AF Liangsheng, X. Chen, W. TI Random T-DNA mutagenesis identifies a Cu-Zn-superoxide dismutase gene as a virulence factor of Sclerotinia sclerotiorum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Liangsheng, X.] Washington State Univ, Pullman, WA 99164 USA. [Chen, W.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S104 EP S104 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401021 ER PT J AU Liangsheng, X Xiang, M White, D Chen, W AF Liangsheng, X. Xiang, M. White, D. Chen, W. TI Oxalate-minus mutants of Sclerotinia sclerotiorum via random mutagenesis retain pathogenicity SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Liangsheng, X.; Xiang, M.; White, D.] Washington State Univ, Pullman, WA 99164 USA. [Chen, W.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S104 EP S104 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401020 ER PT J AU Lin, H Yang, L Civerolo, EL Walker, MA AF Lin, H. Yang, L. Civerolo, E. L. Walker, M. A. TI Proteomic analysis of grapevines in response to Xylella fastidiosa infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lin, H.] ARS, USDA, PWA, Parlier, CA USA. [Yang, L.] Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China. [Walker, M. A.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S105 EP S106 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401030 ER PT J AU Lin, H Glynn, JM Civerolo, EL AF Lin, H. Glynn, J. M. Civerolo, E. L. TI Complete genome sequence analyses and functional predictions for 'Ca. L. solanacearum', the bacterium associated with potato zebra chip disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lin, H.] ARS, USDA, PWA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S105 EP S105 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401029 ER PT J AU Lin, L Li, R Mock, R Kinard, G AF Lin, L. Li, R. Mock, R. Kinard, G. TI Development of a real-time RT-PCR assay to detect Peach latent mosaic viroid infections in stone fruit trees SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lin, L.; Li, R.; Mock, R.; Kinard, G.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S105 EP S105 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401027 ER PT J AU Lin, L Li, R Mock, R Kinard, G AF Lin, L. Li, R. Mock, R. Kinard, G. TI One-step multiplex RT-PCR assay for simultaneous detection of two viroids and Plum bark necrosis stem pitting-associated virus in stone fruit trees SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lin, L.; Li, R.; Mock, R.; Kinard, G.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S105 EP S105 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401026 ER PT J AU Ling, K Fei, Z Wechter, WP Hernamdez, AG AF Ling, K. Fei, Z. Wechter, W. P. Hernamdez, A. G. TI Deep sequencing of small RNAs for virus and viroid identification in tomatoes SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Ling, K.; Wechter, W. P.] ARS, USDA, Charleston, SC USA. [Fei, Z.] Cornell Univ, USDA, ARS, BTI, Ithaca, NY USA. [Hernamdez, A. G.] Univ Illinois, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S106 EP S106 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401031 ER PT J AU Liu, P Lin, H Walker, MA AF Liu, P. Lin, H. Walker, M. A. TI Metabolic profiling of xylem sap from Pierce's disease resistant and susceptible grapevines SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Liu, P.] China Agr Univ, Beijing 100094, Peoples R China. [Lin, H.] ARS, USDA, PWA, Parlier, CA USA. [Walker, M. A.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S107 EP S108 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401042 ER PT J AU Liu, Q Chen, J Munyaneza, J Civerolo, E AF Liu, Q. Chen, J. Munyaneza, J. Civerolo, E. TI Endophytic bacteria in potato tubers affected by zebra chip disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Liu, Q.] S China Agr Univ, Guangzhou, Guangdong, Peoples R China. [Chen, J.; Civerolo, E.] ARS, USDA, PWA, Parlier, CA USA. [Munyaneza, J.] ARS, USDA, Wapato, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S108 EP S108 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401048 ER PT J AU Liu, Z Rappaport, K Bowman, H Twieg, E Mavrodieva, V Levy, L AF Liu, Z. Rappaport, K. Bowman, H. Twieg, E. Mavrodieva, V. Levy, L. TI Evaluation and adaptation of CANARY technology for rapid detection of plant pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Liu, Z.; Rappaport, K.; Bowman, H.; Twieg, E.; Mavrodieva, V.; Levy, L.] USDA, APHIS, PPQ, CPHST,NPGBI, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S106 EP S106 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401032 ER PT J AU Loper, JE Hassan, KA Davis, EW Lim, CK Paulsen, IT AF Loper, J. E. Hassan, K. A. Davis, E. W. Lim, C. K. Paulsen, I. T. TI Comparative genomic analysis reveals new aspects of the biology and secondary metabolism of biological control strains of Pseudomonas spp. SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Loper, J. E.; Davis, E. W.] USDA ARS, Corvallis, OR USA. [Hassan, K. A.; Lim, C. K.; Paulsen, I. T.] Macquarie Univ, Sydney, NSW 2109, Australia. RI Hassan, Karl/B-2802-2013 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S231 EP S231 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401763 ER PT J AU Lynch, S Eskalen, A Zambino, P Scott, T AF Lynch, S. Eskalen, A. Zambino, P. Scott, T. TI Botryosphaeria species complex associated with coast live oak (Quercus agrifolia) mortality in Southern California SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lynch, S.; Eskalen, A.; Scott, T.] Univ Calif Riverside, Riverside, CA 92521 USA. [Zambino, P.] Pacific SW Reg, Forest Serv, San Bernardino, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S111 EP S111 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401062 ER PT J AU Lynch, SC Eskalen, A Zambino, P Scott, T AF Lynch, S. C. Eskalen, A. Zambino, P. Scott, T. TI Ophiostoma and Geosmithia spp. associated with western oak bark beetle damage on declining California black oak and coast live oak in southern California SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lynch, S. C.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. [Scott, T.] Univ Calif Riverside, Dept Earth Sci, Riverside, CA 92521 USA. [Eskalen, A.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Zambino, P.] US Forest Serv, USDA, San Bernardino, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S249 EP S249 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401857 ER PT J AU Lynn, N Leslie, CA Gonzalez, A Sudarshana, MR AF Lynn, N. Leslie, C. A. Gonzalez, A. Sudarshana, M. R. TI Molecular screening of walnut backcross populations for a DNA marker linked to cherry leafroll virus resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lynn, N.; Leslie, C. A.] UC Davis, Davis, CA USA. [Gonzalez, A.; Sudarshana, M. R.] ARS, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S111 EP S111 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401063 ER PT J AU Mahaffee, W Bailey, B Pardyjak, E Skinkis, P Stoll, R AF Mahaffee, W. Bailey, B. Pardyjak, E. Skinkis, P. Stoll, R. TI Pathogen Transport and Response-tool for Agricultural Canopies (P-TRAC) - A modeling system to guide disease management decisions in perennial canopies SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mahaffee, W.] ARS, USDA, HCRL, Corvallis, OR USA. [Bailey, B.; Pardyjak, E.; Stoll, R.] Univ Utah, Dept Mech Engn, Salt Lake City, UT USA. [Skinkis, P.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S112 EP S113 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401072 ER PT J AU Mahaffee, W Grove, G Martin, D Albrecht, A AF Mahaffee, W. Grove, G. Martin, D. Albrecht, A. TI Grower implementation of LAMP PCR to initiate grape powdery mildew fungicide program based on inoculum detection SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mahaffee, W.; Martin, D.] ARS, USDA, HCRL, Corvallis, OR USA. [Grove, G.] Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Albrecht, A.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S112 EP S112 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401071 ER PT J AU Mahaffee, WF Grove, GG Martin, D Albrecht, AS AF Mahaffee, W. F. Grove, G. G. Martin, D. Albrecht, A. S. TI Another step closer to implementing inoculum detection as a method to time initiation of fungicide applications for management of grape powdery mildew SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Albrecht, A. S.] Oregon State Univ, Dept Plant Pathol, Corvallis, OR 97331 USA. [Grove, G. G.] Washington State Univ, Dept Plant Pathol, Prosser, WA USA. [Mahaffee, W. F.; Martin, D.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S249 EP S249 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401858 ER PT J AU Manjunath, KL Ramadugu, C Kund, G Trumble, J Lee, RF AF Manjunath, K. L. Ramadugu, C. Kund, G. Trumble, J. Lee, R. F. TI A tomato model system to study Citrus huanglongbing SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Manjunath, K. L.; Lee, R. F.] ARS, Natl Clonal Germplasm Repository Citrus & Dates, USDA, Riverside, CA USA. [Ramadugu, C.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Kund, G.; Trumble, J.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S113 EP S113 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401074 ER PT J AU Marasas, C AF Marasas, C. TI Managing pest risk of plants for planting in international trade: US import regulations at a crossroad SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Marasas, C.] USDA, APHIS, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S114 EP S114 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401078 ER PT J AU Maroon-Lango, C Turner, RS Brown, H McCallister, JE Barbosa, AA Smither, MR Boulais, VL AF Maroon-Lango, C. Turner, R. S. Brown, H. McCallister, J. E. Barbosa, A. A. Smither, M. R. Boulais, V. L. TI The USDA-APHIS quarantine programs for sugarcane, grasses, rice & bamboo SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Maroon-Lango, C.; Turner, R. S.; Brown, H.; McCallister, J. E.; Barbosa, A. A.; Smither, M. R.; Boulais, V. L.] USDA, Plant Germplasm Quarantine Program, Plant Hlth Programs, APHIS,BARC E, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S114 EP S114 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401081 ER PT J AU Martin, RR Quito-Avila, DF Cline, WO Harmon, PF Brannen, PM AF Martin, R. R. Quito-Avila, D. F. Cline, W. O. Harmon, P. F. Brannen, P. M. TI Blueberry necrotic ring blotch, a new blueberry disease caused by a virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Martin, R. R.] ARS, USDA, Corvallis, OR USA. [Quito-Avila, D. F.] Oregon State Univ, Corvallis, OR 97331 USA. [Cline, W. O.] N Carolina State Univ, Castle Hayne, NC USA. [Harmon, P. F.] Univ Florida, Gainesville, FL USA. [Brannen, P. M.] Univ Georgia, Athens, GA 30602 USA. NR 0 TC 3 Z9 3 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S115 EP S115 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401085 ER PT J AU Martins, MM de Jensen, CE Porch, TG Beaver, J AF Martins, M. Mbui de Jensen, C. Estevez Porch, T. G. Beaver, J. TI Response of Phaseolus vulgaris lines to angular leaf spot SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Martins, M. Mbui; de Jensen, C. Estevez; Beaver, J.] Univ Puerto Rico, Mayaguez, PR USA. [Porch, T. G.] USDA, Trop Agr Res Stn, TARS, Mayaguez, PR USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S278 EP S278 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402067 ER PT J AU Martins, OM Couto, ME Bull, CT AF Martins, O. M. Couto, M. E. Bull, C. T. TI Identification of an emergent bacterial blight of garlic in Brazil SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Martins, O. M.] Embrapa Genet Resources & Biotechnol, Brasilia, DF, Brazil. [Couto, M. E.] Embrapa Clima Temperado, Pelotas, Brazil. [Bull, C. T.] ARS, USDA, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S115 EP S115 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401086 ER PT J AU Maruthachalam, K Klosterman, SJ Subbarao, KV AF Maruthachalam, K. Klosterman, S. J. Subbarao, K. V. TI Colonization of spinach (Spinacia oleracea L.) by GFP-tagged Verticillium dahliae SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Maruthachalam, K.] Univ Calif, Salinas, CA USA. [Klosterman, S. J.] ARS, USDA, Salinas, CA USA. [Subbarao, K. V.] Univ Calif, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S115 EP S115 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401087 ER PT J AU Mathew, F Alananbeh, K Balbyshev, N Heitkamp, E Gulya, T Markell, S AF Mathew, F. Alananbeh, K. Balbyshev, N. Heitkamp, E. Gulya, T. Markell, S. TI Reevaluation of Phomopsis species affecting sunflowers in the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mathew, F.; Alananbeh, K.; Heitkamp, E.; Markell, S.] N Dakota State Univ, Fargo, ND 58105 USA. [Balbyshev, N.; Gulya, T.] ARS, USDA, No Crop Sci Lab, Fargo, ND USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S115 EP S116 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401090 ER PT J AU Mauzey, SJ Davis, CD Martins, OM Koike, ST Bull, CT AF Mauzey, S. J. Davis, C. D. Martins, O. M. Koike, S. T. Bull, C. T. TI A new disease of parsley (Petroselinum crispum) in California caused by a fluorescent pseudomonad related to Pseudomonas viridiflava SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mauzey, S. J.] Calif State Univ, Undergrad Res Opportun Ctr, Seaside, CA USA. [Davis, C. D.] Hartnell Coll, Salinas, CA USA. [Martins, O. M.] Embrapa Genet Resources & Biotechnol, Brasilia, DF, Brazil. [Koike, S. T.] Univ Calif, Davis, CA USA. [Bull, C. T.] ARS, USDA, Salinas, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S116 EP S116 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401095 ER PT J AU Mavrodi, D Hassan, K Mavrodi, O Paulsen, I Loper, J Weller, D Thomashow, L AF Mavrodi, D. Hassan, K. Mavrodi, O. Paulsen, I. Loper, J. Weller, D. Thomashow, L. TI Complete genomes of plant growth-promoting rhizobacteria Pseudomonas fluorescens strains Q8r1-96 and Q2-87 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mavrodi, D.; Mavrodi, O.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Hassan, K.; Paulsen, I.] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. [Loper, J.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. [Weller, D.; Thomashow, L.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. RI Mavrodi, Dmitri/J-2745-2013; OI Hassan, Karl/0000-0003-2031-9679 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S117 EP S117 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401097 ER PT J AU Mavrodi, O Walter, N Taylor, C Okubara, P AF Mavrodi, O. Walter, N. Taylor, C. Okubara, P. TI Biological control properties of Pseudomonas isolates SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mavrodi, O.] Washington State Univ, Pullman, WA 99164 USA. [Walter, N.; Okubara, P.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. [Taylor, C.] Ohio State Univ, OARDC Plant Pathol, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S117 EP S117 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401096 ER PT J AU Mavrodieva, V Williams, K Negi, S Levy, L AF Mavrodieva, V. Williams, K. Negi, S. Levy, L. TI Characterization of a rare Plum pox virus W isolate found in germplasm illegally carried to the US SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mavrodieva, V.; Williams, K.; Negi, S.; Levy, L.] USDA, APHIS, PPQ, CPHST, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S117 EP S117 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401098 ER PT J AU Mazzola, M AF Mazzola, M. TI Particle size affects Brassica juncea seed meal-induced pathogen suppression of Rhizoctonia solani AG-5 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S249 EP S249 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401861 ER PT J AU Mazzola, M AF Mazzola, M. TI Resident biology restricts proliferation of Macrophomina phaseolina in brassicaceae seed meal amended soil SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S249 EP S249 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401860 ER PT J AU Mazzola, M AF Mazzola, M. TI Advances in Brassicaceae seed meal formulation and application for replant disease control in organic apple orchards SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S117 EP S117 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401100 ER PT J AU Mazzola, M AF Mazzola, M. TI Active manipulation of resident biology to suppress Macrophomina phaseolina in strawberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S117 EP S117 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401099 ER PT J AU Medrano, EG Esquivel, JF Bell, AA AF Medrano, E. G. Esquivel, J. F. Bell, A. A. TI Transmission of the opportunistic cotton (Gossypium hirsutum L.) boll pathogen Pantoea agglomerans by the brown stink bug (Euschistus servus Say) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Medrano, E. G.; Bell, A. A.] ARS, USDA, Cotton Pathol Res Unit, College Stn, TX USA. [Esquivel, J. F.] ARS, USDA, Areawide Pest Management Res Unit, College Stn, TX USA. NR 0 TC 3 Z9 3 U1 4 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S119 EP S119 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401109 ER PT J AU Melnick, RL Marelli, J Bailey, BA AF Melnick, R. L. Marelli, J. Bailey, B. A. TI The molecular interaction of Theobroma cacao and Moniliophthora perniciosa, causal agent of witches' broom, during infection of young pods SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Marelli, J.] Mars Ctr Cocoa Sci, BR-4630000 Itajuipe, Bahia, Brazil. [Melnick, R. L.; Bailey, B. A.] ARS, USDA, Sustainable Perennial Crops Lab, PSI, Beltsville, MD USA. RI Melnick, Rachel/B-1719-2013 OI Melnick, Rachel/0000-0002-5113-2551 NR 0 TC 1 Z9 1 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S274 EP S274 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402050 ER PT J AU Mersha, Z Aime, MC Cannon, P Nandwani, D Nelson, S Spaine, PC Schlub, RL AF Mersha, Z. Aime, M. C. Cannon, P. Nandwani, D. Nelson, S. Spaine, P. C. Schlub, R. L. TI Decline of Casuarina equisetifolia (ironwood) trees on Guam: Ganoderma and Phellinus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Mersha, Z.] Univ Florida, Homestead, FL USA. [Aime, M. C.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Cannon, P.] US Forest Serv, USDA, Vallejo, CA USA. [Nandwani, D.] No Marianas Coll, Saipan, CM USA. [Nelson, S.] Univ Hawaii, Manoa, HI USA. [Spaine, P. C.] USDA APHIS BRS, Riverdale, MD USA. [Schlub, R. L.] Univ Guam, Mangilao, GU USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S216 EP S216 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401679 ER PT J AU Meyer, SL Everts, KL Gardener, BBM AF Meyer, S. L. Everts, K. L. Gardener, B. B. McSpadden TI Effects of green manures on nematode population densities in an organic tomato field SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Meyer, S. L.] ARS, USDA, Beltsville, MD USA. [Everts, K. L.] Univ Maryland, Salisbury, MD USA. [Everts, K. L.] Univ Delaware, Salisbury, MD USA. [Gardener, B. B. McSpadden] Ohio State Univ, OARDC, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S120 EP S120 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401115 ER PT J AU Nakhla, MK Picton, DD Owens, KJ Levy, L AF Nakhla, M. K. Picton, D. D. Owens, K. J. Levy, L. TI Development and validation of a multiplex one-step RT-PCR for the improved detection of potyviruses infecting imported germplasm SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Picton, D. D.; Owens, K. J.; Levy, L.] USDA, APHIS, PPQ, CPHST,NPGBL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S125 EP S125 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401148 ER PT J AU Nakhla, MK Li, W Wei, G Levy, L AF Nakhla, M. K. Li, W. Wei, G. Levy, L. TI Development and validation of Citrus leprosis virus-C (CiLV-C) molecular detection and identification methods for use in regulatory diagnostic assays SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Li, W.; Wei, G.; Levy, L.] USDA, APHIS, PPQ, CPHST,NPGBL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S125 EP S125 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401147 ER PT J AU Natarajan, S Chen, X MacDonald, M Garrett, W Matthews, B AF Natarajan, S. Chen, X. MacDonald, M. Garrett, W. Matthews, B. TI Proteome reference map for the soybean cyst nematode SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Natarajan, S.] ARS, USDA, PSI, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S126 EP S126 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401149 ER PT J AU Natilla, A Nemchinov, LG Vaira, A Hammond, J Hammond, RW AF Natilla, A. Nemchinov, L. G. Vaira, A. Hammond, J. Hammond, R. W. TI Virus-like particles of Maize rayado fino virus, Cucumber mosaic virus, and Lolium latent virus as chemical bio-conjugate substrates SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Natilla, A.; Nemchinov, L. G.; Hammond, R. W.] ARS, USDA, PSI, MPPL, Beltsville, MD USA. [Vaira, A.] CNR, Ist Virol Vegetale, I-10126 Turin, Italy. [Hammond, J.] ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S126 EP S126 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401150 ER PT J AU Newcomb, M Acevedo, M Bockelman, HE Brown-Guedira, G Goates, BJ Jackson, EW Jin, Y Njau, P Singh, D Wanyera, R Bonman, J AF Newcomb, M. Acevedo, M. Bockelman, H. E. Brown-Guedira, G. Goates, B. J. Jackson, E. W. Jin, Y. Njau, P. Singh, D. Wanyera, R. Bonman, J. TI Resistance to the stem rust 'Ug99' race group in spring wheat landrace accessions from the USDA-ARS National Small Grains Collection (NSGC) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Newcomb, M.] ARS, USDA, Aberdeen, ID USA. [Acevedo, M.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Bockelman, H. E.; Goates, B. J.; Bonman, J.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA. [Brown-Guedira, G.] ARS, USDA, Eastern Reg Genotyping Lab, Raleigh, NC USA. [Jin, Y.] Univ Minnesota, ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Jin, Y.] Univ Minnesota, ARS, USDA, Dept Plant Pathol, St Paul, MN 55108 USA. [Njau, P.; Wanyera, R.] Kenya Agr Res Inst, Njoro, Kenya. [Singh, D.] Univ Sydney, Plant Breeding Inst, Sydney, NSW 2006, Australia. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S127 EP S128 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401160 ER PT J AU Ni, X AF Ni, X. TI Spatial and temporal patterns of insect damage and aflatoxin contamination in corn at pre-harvest SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Ni, X.] ARS, USDA, Crop Gent & Breeding Res Unit, Tifton, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S128 EP S128 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401162 ER PT J AU Nischwitz, C Schmitt, M Skantar, A Bunderson, T McClure, M AF Nischwitz, C. Schmitt, M. Skantar, A. Bunderson, T. McClure, M. TI Root-knot nematode species in golf course greens in the Western USA SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Nischwitz, C.; Bunderson, T.] Utah State Univ, Logan, UT 84322 USA. [Schmitt, M.; McClure, M.] Univ Arizona, Tucson, AZ USA. [Skantar, A.] ARS, USDA, Nematol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S129 EP S129 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401167 ER PT J AU Nishijima, KA Wall, MM Keith, LM AF Nishijima, K. A. Wall, M. M. Keith, L. M. TI Microorganisms and antifungal properties associated with noni (Morinda citrifolia) fruit and fermented juice in Hawaii SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Nishijima, K. A.; Wall, M. M.; Keith, L. M.] ARS, USDA, Hilo, HI USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S129 EP S129 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401168 ER PT J AU Oak, S Hwang, J Jeffers, S AF Oak, S. Hwang, J. Jeffers, S. TI An in vitro baiting assay for recovery of Phytophthora ramorum from waterways SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Oak, S.] US Forest Serv, USDA, Asheville, NC USA. [Hwang, J.; Jeffers, S.] Clemson Univ, Clemson, SC USA. NR 0 TC 1 Z9 1 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S131 EP S131 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401179 ER PT J AU Olanya, M Larkin, R Zhongqi, H Jain, S AF Olanya, M. Larkin, R. Zhongqi, H. Jain, S. TI Survival potential of Phytophthora infestans sporangia in relation to meteorological factors SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Olanya, M.; Larkin, R.; Zhongqi, H.] ARS, USDA, Orono, ME USA. [Jain, S.] Univ Maine, Orono, ME USA. RI Jain, Shaleen/B-2923-2011 OI Jain, Shaleen/0000-0003-1792-4421 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S132 EP S132 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401184 ER PT J AU Ortega-Beltran, A Grubisha, L Cotty, PJ AF Ortega-Beltran, A. Grubisha, L. Cotty, P. J. TI Genetic diversity within a vegetative compatibility group of aflatoxin-producing fungi SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Ortega-Beltran, A.] Univ Arizona, Sch Plant Sci Tucson, Tucson, AZ 85721 USA. [Grubisha, L.; Cotty, P. J.] ARS, USDA, Sch Plant Sci, Tucson, AZ USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S250 EP S250 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401863 ER PT J AU Ortega-Beltran, A Jaime-Garcia, R Cotty, PJ AF Ortega-Beltran, A. Jaime-Garcia, R. Cotty, P. J. TI Aflatoxin-producing fungi in maize fields of Sonora, Mexico at varying elevations: A three year study SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Cotty, P. J.] Univ Arizona, ARS, USDA, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S133 EP S134 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401196 ER PT J AU Osman, F Al Rwahnih, M Pitman, T Cordero, F Preece, J Golino, D AF Osman, F. Al Rwahnih, M. Pitman, T. Cordero, F. Preece, J. Golino, D. TI A preliminary account of the sanitary status of Prunus species in the National Clonal Germplasm Repository SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Osman, F.; Al Rwahnih, M.; Cordero, F.; Golino, D.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Pitman, T.] Univ Calif Davis, Fdn Plant Serv, Davis, CA 95616 USA. [Preece, J.] ARS, Natl Clonal Germplasm Repository, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S134 EP S134 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401198 ER PT J AU Otrosina, W Sung, S Kliejunas, J Woodruff, W Spaine, P AF Otrosina, W. Sung, S. Kliejunas, J. Woodruff, W. Spaine, P. TI Blackstain root disease effects on foliar nutrients, chlorophyll content, and internodal growth in ponderosa pine SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sung, S.] US Forest Serv, USDA, Pineville, LA USA. [Kliejunas, J.] US Forest Serv, USDA, Kent, WA USA. [Woodruff, W.] US Forest Serv, USDA, Susanville, CA USA. [Spaine, P.] USDA APHIS, Riverdale, MD USA. [Otrosina, W.] US Forest Serv, USDA, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S135 EP S135 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401203 ER PT J AU Palencia, ER Mitchell, TR Riley, RT Hinton, DM Snook, ME Bacon, C AF Palencia, E. R. Mitchell, T. R. Riley, R. T. Hinton, D. M. Snook, M. E. Bacon, C. TI Endophytic associations and production of mycotoxins by the Aspergillus section Nigri species SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Palencia, E. R.] Univ Georgia, USDA, ARS, Russell Res Ctr, Athens, GA 30602 USA. [Palencia, E. R.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Mitchell, T. R.; Hinton, D. M.; Snook, M. E.; Bacon, C.] ARS, USDA, Russell Res Ctr, Athens, GA USA. [Riley, R. T.] ARS, USDA, Toxicol & Mycotoxin Res Unit, Athens, GA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S135 EP S135 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401207 ER PT J AU Park, J Cavender-Bares, J Juzwik, J AF Park, J. Cavender-Bares, J. Juzwik, J. TI Effect of multiple Ceratcystis smalleyi infections on stem water conductance in maturing bitternut hickory SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Juzwik, J.] US Forest Serv, St Paul, MN USA. [Park, J.; Cavender-Bares, J.] Univ Minnesota, St Paul, MN 55108 USA. RI Cavender-Bares, Jeannine/K-5716-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S245 EP S245 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401839 ER PT J AU Parke, JL Britton, KO Frankel, SJ AF Parke, J. L. Britton, K. O. Frankel, S. J. TI Historical pathways of introduction for non-indigenous forest pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Parke, J. L.] Oregon State Univ, Corvallis, OR 97331 USA. [Britton, K. O.] US Forest Serv, USDA, Arlington, VA USA. [Frankel, S. J.] US Forest Serv, USDA, PSW Res Stn, Albany, CA USA. RI Parke, Jennifer/H-8464-2014 NR 0 TC 0 Z9 0 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S137 EP S138 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401219 ER PT J AU Parke, JL Eberhart, JE Hansen, EM Frankel, SJ AF Parke, J. L. Eberhart, J. E. Hansen, E. M. Frankel, S. J. TI Forest Phytophthoras of the World website SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Parke, J. L.; Eberhart, J. E.; Hansen, E. M.] Oregon State Univ, Corvallis, OR 97331 USA. [Frankel, S. J.] US Forest Serv, USDA, PSW Res Stn, Albany, CA USA. RI Parke, Jennifer/H-8464-2014 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S137 EP S137 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401218 ER PT J AU Pastor-Corrales, MA Osorno, JM Markell, SG Goswami, RS AF Pastor-Corrales, M. A. Osorno, J. M. Markell, S. G. Goswami, R. S. TI Using phenotypic markers to identify common beans with two and three rust resistance genes SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pastor-Corrales, M. A.] USDA ARS, Beltsville, MD USA. [Osorno, J. M.; Markell, S. G.; Goswami, R. S.] N Dakota State Univ, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S138 EP S138 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401221 ER PT J AU Pedley, KF Pandey, AK Kendrick, MD Zhang, C Graham, MA Whitham, SA Hill, JH AF Pedley, K. F. Pandey, A. K. Kendrick, M. D. Zhang, C. Graham, M. A. Whitham, S. A. Hill, J. H. TI Functional analysis of Asian soybean rust resistance pathways using virus-induced gene silencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pedley, K. F.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. [Pandey, A. K.] Iowa State Univ, USDA, ARS, Ft Detrick, MD USA. [Zhang, C.; Whitham, S. A.; Hill, J. H.] Iowa State Univ, Ames, IA USA. [Graham, M. A.] ARS, USDA, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S139 EP S139 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401227 ER PT J AU Peduto, F Sutherland, AM Hand, EK Broome, JC Parikh, PD Bettiga, LJ Smith, RJ Mahaffee, WF Gubler, WD AF Peduto, F. Sutherland, A. M. Hand, E. K. Broome, J. C. Parikh, P. D. Bettiga, L. J. Smith, R. J. Mahaffee, W. F. Gubler, W. D. TI Comparing the efficiency of visual scouting, spore trapping systems and a bioindicator for early detection of Erysiphe necator in California vineyards SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Peduto, F.; Sutherland, A. M.; Hand, E. K.; Broome, J. C.; Parikh, P. D.; Gubler, W. D.] Univ Calif Davis, Davis, CA 95616 USA. [Bettiga, L. J.] UC Cooperat Extens Monterey Cty, Salinas, CA USA. [Smith, R. J.] UC Cooperat Extens Sonoma Cty, Santa Rosa, CA USA. [Mahaffee, W. F.] ARS, USDA, HCRL, Corvallis, OR USA. RI Hand, Francesca/D-1761-2013 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S139 EP S139 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401228 ER PT J AU Perumal, R Little, CR Erraitaimuthu, SR Prom, LK Magill, CW AF Perumal, R. Little, C. R. Erraitaimuthu, S. R. Prom, L. K. Magill, C. W. TI Genetic diversity and pathotype determination of Colletotrichum sublineolum isolated causing anthracnose disease in sorghum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Perumal, R.] Kansas State Univ, Hays, KS USA. [Little, C. R.; Erraitaimuthu, S. R.] Kansas State Univ, Manhattan, KS 66506 USA. [Prom, L. K.] ARS, USDA, College Stn, TX USA. [Magill, C. W.] Texas A&M Univ, College Stn, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S140 EP S141 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401237 ER PT J AU Perumal, R Little, CR Erraittaimuthu, SR Prom, LK Magill, CW AF Perumal, R. Little, C. R. Erraittaimuthu, S. R. Prom, L. K. Magill, C. W. TI Virulence and molecular genotyping studies of Sporisorium relianum isolates in sorghum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Perumal, R.; Little, C. R.; Erraittaimuthu, S. R.] Kansas State Univ, Manhattan, KS 66506 USA. [Prom, L. K.] ARS, USDA, College Stn, TX USA. [Magill, C. W.] Texas A&M Univ, College Stn, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S140 EP S140 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401236 ER PT J AU Peter, K Jurick, WM Vico, I Park, E Gaskins, VL Janisiewicz, WJ Conway, WS AF Peter, K. Jurick, W. M. Vico, I. Park, E. Gaskins, V. L. Janisiewicz, W. J. Conway, W. S. TI Host modification of Penicillium solitum during postharvest decay of apple fruit SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Peter, K.; Jurick, W. M.; Park, E.; Gaskins, V. L.; Conway, W. S.] ARS, USDA, Beltsville, MD USA. [Vico, I.] Univ Belgrade, Fac Agr, Inst Phytomed, Belgrade, Serbia. [Janisiewicz, W. J.] ARS, USDA, Kearneysville, WV USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S141 EP S141 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401238 ER PT J AU Peterson, GL Pedley, KF Bockus, WW Stack, JP Cruz, CD Valent, BS AF Peterson, G. L. Pedley, K. F. Bockus, W. W. Stack, J. P. Cruz, C. D. Valent, B. S. TI Susceptibility of select US winter wheat cultivars to wheat blast (Magnaporthe oryzae) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Peterson, G. L.; Pedley, K. F.] ARS, USDA, NAA, FDWSRU, Ft Detrick, MD USA. [Bockus, W. W.; Stack, J. P.; Cruz, C. D.; Valent, B. S.] Kansas State Univ, Manhattan, KS 66506 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S141 EP S141 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401239 ER PT J AU Pethybridge, SJ Gent, D Hay, FS AF Pethybridge, S. J. Gent, D. Hay, F. S. TI Effects of seedborne and overwintering inoculum on ray blight severity in pyrethrum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pethybridge, S. J.] Bot Resources Australia, Ulverstone, Australia. [Gent, D.] ARS, USDA, NFSPRC, Corvallis, OR USA. [Hay, F. S.] Univ Tasmania, Burnie, Tas 7320, Australia. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S141 EP S141 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401240 ER PT J AU Pokharel, R Li, R Mock, RG AF Pokharel, R. Li, R. Mock, R. G. TI Genetic variability of Colorado Cherry rasp leaf virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pokharel, R.] Colorado State Univ, Grand Junction, CO USA. [Mock, R. G.] ARS, USDA, NGRL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S142 EP S143 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401248 ER PT J AU Pokharel, R Mock, RG Kinard, G Li, R AF Pokharel, R. Mock, R. G. Kinard, G. Li, R. TI Incidence of multiple viruses in western Colorado cherry orchards SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pokharel, R.] Colorado State Univ, Grand Junction, CO USA. [Mock, R. G.] ARS, USDA, NGRL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S143 EP S143 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401249 ER PT J AU Polashock, J Vorsa, N Johnson-Cicalese, J Tadych, M White, J AF Polashock, J. Vorsa, N. Johnson-Cicalese, J. Tadych, M. White, J. TI Fruit rot resistance and heritability in cultivated cranberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Polashock, J.] ARS, USDA, Chatsworth, NJ USA. [Vorsa, N.; Johnson-Cicalese, J.] Rutgers State Univ, Chatsworth, NJ USA. [Tadych, M.; White, J.] Rutgers State Univ, New Brunswick, NJ 08903 USA. RI Tadych, Mariusz/G-4449-2015 OI Tadych, Mariusz/0000-0003-4776-1096 NR 0 TC 0 Z9 0 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S143 EP S143 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401252 ER PT J AU Polashock, J Constantelos, C Oudemans, P AF Polashock, J. Constantelos, C. Oudemans, P. TI Impact and characterization of 'black shadow' on highbush blueberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Polashock, J.] ARS, USDA, Chatsworth, NJ USA. [Constantelos, C.; Oudemans, P.] Rutgers State Univ, Chatsworth, NJ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S143 EP S143 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401251 ER PT J AU Poojari, S Alabi, OJ Walsh, DB Okubara, P Naidu, RA AF Poojari, S. Alabi, O. J. Walsh, D. B. Okubara, P. Naidu, R. A. TI Rapid and real-time detection of grapevine leafroll associated viruses in grapevines and insect vectors SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Poojari, S.; Alabi, O. J.; Walsh, D. B.] Washington State Univ, Dept Entomol, Prosser, WA USA. [Okubara, P.] Washington State Univ, USDA, ARS, Dept Plant Pathol, Pullman, WA 99164 USA. [Naidu, R. A.] Washington State Univ, Irrigated Agr Res & Exten Ctr, Prosser, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S144 EP S144 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401255 ER PT J AU Poole, G Smiley, R Paulitz, TC Garland-Campbell, K AF Poole, G. Smiley, R. Paulitz, T. C. Garland-Campbell, K. TI Identifying quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in two spring wheat populations SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Poole, G.] Washington State Univ, Pullman, WA 99164 USA. [Smiley, R.] Oregon State Univ, Pendleton, OR USA. [Paulitz, T. C.; Garland-Campbell, K.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S144 EP S144 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401256 ER PT J AU Porter, LD Walsh, DB Boydston, RA O'Donnell, K AF Porter, L. D. Walsh, D. B. Boydston, R. A. O'Donnell, K. TI Survival and natural biological control of Sclerotinia sclerotiorum sclerotia in alfalfa seed production SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Porter, L. D.; Boydston, R. A.] ARS, USDA, Prosser, WA USA. [Walsh, D. B.] Washington State Univ, Prosser, WA USA. [O'Donnell, K.] ARS, USDA, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S144 EP S144 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401258 ER PT J AU Preuett, JA Collins, DJ Luster, DG Widmer, TL AF Preuett, J. A. Collins, D. J. Luster, D. G. Widmer, T. L. TI The effects of salinity on Phytophthora ramorum viability and infectivity SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Preuett, J. A.; Collins, D. J.] So Univ, Urban Forestry Program, Baton Rouge, LA USA. [Preuett, J. A.; Collins, D. J.] A&M Coll, Baton Rouge, LA USA. [Luster, D. G.; Widmer, T. L.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. NR 0 TC 1 Z9 1 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S146 EP S146 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401267 ER PT J AU Pusey, P Stockwell, VO Reardon, CL Smits, TH Duffy, B AF Pusey, P. Stockwell, V. O. Reardon, C. L. Smits, T. H. Duffy, B. TI Antibiosis by Pantoea agglomerans biocontrol strain E325 against Erwinia amylovora on apple flower stigmas SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Pusey, P.] ARS, USDA, Wenatchee, WA USA. [Stockwell, V. O.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Reardon, C. L.] ARS, USDA, Columbia Plateau Conservat Res Ctr, Adams, OR USA. [Smits, T. H.; Duffy, B.] Swiss Fed Res Stn, CH-8820 Wadenswil, Switzerland. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S146 EP S147 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401271 ER PT J AU Qiu, D Vandemark, G Chen, W AF Qiu, D. Vandemark, G. Chen, W. TI Comparative transcriptome analysis in Sclerotinia sclerotiorum and S-trifoliorum by 454 Titanium RNA sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Qiu, D.] Washington State Univ, Pullman, WA 99164 USA. [Vandemark, G.; Chen, W.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S148 EP S148 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401282 ER PT J AU Qiu, D Vandemark, G Chen, W AF Qiu, D. Vandemark, G. Chen, W. TI Comparative analyses of endogenous small RNAs in Sclerotinia sclerotiorum and S-trifoliorum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Qiu, D.] Washington State Univ, Pullman, WA 99164 USA. [Vandemark, G.; Chen, W.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S148 EP S148 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401281 ER PT J AU Quito-Alva, DF Martin, RR AF Quito-Alva, D. F. Martin, R. R. TI Significant increase in titer of Raspberry bushy dwarf virus when present with Raspberry leaf mottle virus and its effect on raspberry plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Quito-Alva, D. F.] Oregon State Univ, Corvallis, OR 97331 USA. [Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S149 EP S149 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401288 ER PT J AU Quito-Alva, DF Martin, RR AF Quito-Alva, D. F. Martin, R. R. TI Raspberry latent virus a plant reovirus that is aphid transmitted in a replicative persistent manner SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Quito-Alva, D. F.] Oregon State Univ, Corvallis, OR 97331 USA. [Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S149 EP S149 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401287 ER PT J AU Radwan, O Govindarajulu, M Wu, X Libault, M Neece, D Berg, R Huber, SC Stacey, G Taylor, CG Steven, CJ AF Radwan, O. Govindarajulu, M. Wu, X. Libault, M. Neece, D. Berg, R. Huber, S. C. Stacey, G. Taylor, C. G. Steven, C. J. TI A 14-3-3 protein appears to be required for establishing normal nodulation in soybean SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wu, X.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Govindarajulu, M.] Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA. [Libault, M.; Stacey, G.] Univ Missouri, Sch Med, Dept Mol Microbiol & Immunol, Columbia, MO 65212 USA. [Neece, D.; Huber, S. C.; Steven, C. J.] ARS, Dept Crop Sci, Urbana, IL USA. [Neece, D.; Huber, S. C.; Steven, C. J.] ARS, USDA, Urbana, IL USA. [Berg, R.] Danforth Plant Sci Ctr, St Louis, MO USA. [Taylor, C. G.] Ohio State Univ, Dept Plant Pathol, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S150 EP S150 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401290 ER PT J AU Radwan, O Clough, SJ AF Radwan, O. Clough, S. J. TI Soybean susceptible leaves response to Fusarium virguliforme toxin in a manner resembling an incompatible interaction SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Radwan, O.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Clough, S. J.] ARS, Dept Crop Sci, Urbana, IL USA. [Clough, S. J.] ARS, USDA, Urbana, IL USA. NR 0 TC 0 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S149 EP S150 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401289 ER PT J AU Ramadugu, C Manjunath, KL Razi, MF Khan, IA Roose, M Lee, RF AF Ramadugu, C. Manjunath, K. L. Razi, M. F. Khan, I. A. Roose, M. Lee, R. F. TI Analysis of Citrus Huanglongbing-associated Candidatus Liberibacter strains from Pakistan SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Ramadugu, C.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Manjunath, K. L.; Lee, R. F.] ARS, USDA, Riverside, CA USA. [Razi, M. F.; Khan, I. A.] Univ Agr Faisalabad, Faisalabad, Pakistan. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S150 EP S150 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401292 ER PT J AU Razi, MF Manjunath, KL Ramadugu, C Jaskani, MJ Basra, SA Roose, M Khan, IA Lee, RF AF Razi, M. F. Manjunath, K. L. Ramadugu, C. Jaskani, M. J. Basra, S. A. Roose, M. Khan, I. A. Lee, R. F. TI Detection of 'Candidatus Liberibacter asiaticus' in psyllid and citrus hosts in Pakistan and analysis of psyllid populations SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Razi, M. F.; Jaskani, M. J.; Basra, S. A.; Khan, I. A.] Univ Agr Faisalabad, Faisalabad, Pakistan. [Manjunath, K. L.; Lee, R. F.] ARS, USDA, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. [Ramadugu, C.; Roose, M.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. RI Jaskani, Muhammad/A-8577-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S151 EP S151 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401297 ER PT J AU Redinbaugh, MG Jones, MW Boyd, EC AF Redinbaugh, M. G. Jones, M. W. Boyd, E. C. TI Responses of maize (Zea mays L.) near isogenic lines carrying Wsm1, Wsm2 and Wsm3 to three viruses in the Potyviridae SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Redinbaugh, M. G.; Jones, M. W.] ARS Corn & Soybean Res, USDA, Wooster, OH USA. [Boyd, E. C.] Ohio State Univ OARDC, Dept Plant Pathol, Wooster, OH USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S151 EP S151 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401299 ER PT J AU Robertson, N Quito-Avila, DF Keller, KE Martin, RR AF Robertson, N. Quito-Avila, D. F. Keller, K. E. Martin, R. R. TI A novel vitivirus isolated from Ribes species in Alaska SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Robertson, N.] ARS, USDA, Palmer, AK USA. [Quito-Avila, D. F.] Oregon State Univ, Corvallis, OR 97331 USA. [Keller, K. E.; Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S154 EP S154 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401314 ER PT J AU Robertson, NL AF Robertson, N. L. TI Molecular characterization of Tobacco rattle virus RNA1 from Dicentra spectabilis (L.) Lem (bleeding-heart) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Robertson, N. L.] ARS, USDA, Palmer, AK USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S154 EP S154 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401315 ER PT J AU Rogers, E AF Rogers, E. TI Evaluation of Arabidopsis thaliana as a model host for Xylella fastidiosa SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Rogers, E.] ARS, USDA, SJVASC, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S155 EP S155 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401321 ER PT J AU Rolshausen, PE Baumgartner, K Travadon, R Fujiyoshi, P Mahoney, N Molyneux, R Wilcox, W AF Rolshausen, P. E. Baumgartner, K. Travadon, R. Fujiyoshi, P. Mahoney, N. Molyneux, R. Wilcox, W. TI Species identification of the causal agent of Eutypa dieback of grapevine in northeastern US and southeastern Canadian vineyards SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Rolshausen, P. E.] Univ Calif Riverside, Riverside, CA 92521 USA. [Baumgartner, K.] ARS, USDA, Davis, CA USA. [Travadon, R.; Fujiyoshi, P.] Univ Calif Davis, Davis, CA 95616 USA. [Mahoney, N.] ARS, USDA, Albany, CA USA. [Molyneux, R.] Univ Hawaii, Hilo, HI 96720 USA. [Wilcox, W.] Cornell Univ, Geneva, NY USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S156 EP S156 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401325 ER PT J AU Rosskopf, E Kokalis-Burelle, N He, Z Noling, J Butler, D Iriarte, F Booker, B Sances, F AF Rosskopf, E. Kokalis-Burelle, N. He, Z. Noling, J. Butler, D. Iriarte, F. Booker, B. Sances, F. TI ASI-261: A potential non-fumigant alternative to methy bromide SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Rosskopf, E.; Kokalis-Burelle, N.] ARS, USDA, Ft Pierce, FL USA. [He, Z.] Univ Florida, IRREC, Ft Pierce, FL USA. [Noling, J.] Univ Florida, CREC, Lake Alfred, FL USA. [Butler, D.] Univ Tennessee, Knoxville, TN USA. [Iriarte, F.] Kansas State Univ, Manhattan, KS 66506 USA. [Iriarte, F.] Pacific Ag Grp, Dover, FL USA. [Sances, F.] Pacific Ag Grp, San Luis Obispo, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S156 EP S156 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401328 ER PT J AU Roy, A Choudhary, N Hartung, JS Brlansky, RH AF Roy, A. Choudhary, N. Hartung, J. S. Brlansky, R. H. TI Genome sequence of an unassigned Citrus tristeza virus genotypic isolate from Puerto Rico reveals a trifoliate resistance breaking genotype SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Roy, A.; Choudhary, N.; Brlansky, R. H.] Univ Florida, Lake Alfred, FL USA. [Hartung, J. S.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S156 EP S156 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401329 ER PT J AU Roychowdhury, M Jia, Y Jia, M Fjellstrom, R Cartwright, R AF Roychowdhury, M. Jia, Y. Jia, M. Fjellstrom, R. Cartwright, R. TI Characterization of the Pi-b rice blast resistance gene in the National Small Grains Collection (NSGC) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Roychowdhury, M.] Univ Arkansas, Stuttgart, AR USA. [Jia, Y.; Jia, M.; Fjellstrom, R.] ARS, USDA, Stuttgart, AR USA. [Cartwright, R.] Univ Arkansas, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S156 EP S157 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401330 ER PT J AU Royer, TA Giles, KL Elliott, NC Hunger, RL Carver, BF Edwards, JT Armstrong, J AF Royer, T. A. Giles, K. L. Elliott, N. C. Hunger, R. L. Carver, B. F. Edwards, J. T. Armstrong, J. TI IPM programs for winter wheat in Oklahoma: A team approach to manage insects, diseases and weeds SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Royer, T. A.; Giles, K. L.; Hunger, R. L.; Carver, B. F.; Edwards, J. T.; Armstrong, J.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Elliott, N. C.] ARS, USDA, Stillwater, OK USA. RI Royer, Tom/D-9737-2016 OI Royer, Tom/0000-0003-0912-7115 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S157 EP S157 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401331 ER PT J AU Rubio, I Bull, CT AF Rubio, I. Bull, C. T. TI Previous reports of bacterial diseases on crucifers attributed to Pseuomonas syringae pv. maculicola were caused by P. cannabina pv. alisalensis SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 Calif State Univ, Undergrad Res Opportun Ctr, Seaside, CA USA. [Bull, C. T.] ARS, USDA, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S157 EP S157 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401332 ER PT J AU Rudolph, K Bolton, MD Nelson, BD AF Rudolph, K. Bolton, M. D. Nelson, B. D. TI Impact of soybean cyst nematode on Rhizoctonia root and crown rot of sugar beet SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Rudolph, K.; Nelson, B. D.] N Dakota State Univ, Fargo, ND 58105 USA. [Bolton, M. D.] ARS, USDA, No Crop Sci Lab, Fargo, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S157 EP S157 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401333 ER PT J AU Sabanadzovic, S Valverde, RA Wintermantel, WM AF Sabanadzovic, S. Valverde, R. A. Wintermantel, W. M. TI Molecular characterization of an endornavirus from Cucumis spp. SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sabanadzovic, S.] Mississippi State Univ, Dept Biochem Mol Bio Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Valverde, R. A.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Wintermantel, W. M.] ARS, USDA, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S158 EP S158 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401339 ER PT J AU Sabanadzovic, S Keller, KE Martin, RR Tzanetakis, IE AF Sabanadzovic, S. Keller, K. E. Martin, R. R. Tzanetakis, I. E. TI Identification and characterization of a new ampelovirus infecting cultivated and wild blackberries SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sabanadzovic, S.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Keller, K. E.; Martin, R. R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR USA. [Tzanetakis, I. E.] Univ Arkansas, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S158 EP S158 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401338 ER PT J AU Saito, S Cadle-Davidson, L Wilcox, WF AF Saito, S. Cadle-Davidson, L. Wilcox, W. F. TI Practical resistance to fenhexamid Botrytis cinerea isolates from grapevines in New York SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Saito, S.; Wilcox, W. F.] Cornell Univ NYSAES, Geneva, NY USA. [Cadle-Davidson, L.] ARS, USDA, Geneva, NY USA. NR 0 TC 3 Z9 3 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S158 EP S158 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401342 ER PT J AU Schlub, RL Moore, A Marx, B Schlub, K Kennaway, L Quintanilla, M Putnam, M Mersha, Z AF Schlub, R. L. Moore, A. Marx, B. Schlub, K. Kennaway, L. Quintanilla, M. Putnam, M. Mersha, Z. TI Decline of Casuarina equisetifolia (ironwood) trees on Guam: Symptomatology and explanatory variables SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Schlub, R. L.; Moore, A.] Univ Guam, Mangilao, GU USA. [Marx, B.; Schlub, K.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Kennaway, L.] USDA APHIS PPQ CPHST, Ft Collins, CO USA. [Quintanilla, M.] No Marianas Coll, Saipan, CM USA. [Putnam, M.] Oregon State Univ, Corvallis, OR 97331 USA. [Mersha, Z.] Univ Florida, Homestead, FL USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S216 EP S216 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401678 ER PT J AU Schmidt, LS Bhat, RG Browne, GT AF Schmidt, L. S. Bhat, R. G. Browne, G. T. TI Characterization of Cylindrocarpon populations associated with replant disease of almond and peach SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Schmidt, L. S.; Browne, G. T.] USDA ARS, Davis, CA USA. [Bhat, R. G.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S160 EP S161 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401355 ER PT J AU Schroeder, KL Shetty, KK Paulitz, TC AF Schroeder, K. L. Shetty, K. K. Paulitz, T. C. TI Survey of Rhizoctonia spp. from wheat soils in the US and determination of pathogenicity on wheat and barley SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Schroeder, K. L.] Washington State Univ, Pullman, WA 99164 USA. [Shetty, K. K.] Syngenta Seed Care, Durham, NH USA. [Paulitz, T. C.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 2 Z9 2 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S161 EP S161 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401359 ER PT J AU Sengoda, VG Munyaneza, JE Buchman, JL Fisher, TW Pappu, HR AF Sengoda, V. G. Munyaneza, J. E. Buchman, J. L. Fisher, T. W. Pappu, H. R. TI Effects of temperature on potato zebra chip disease development SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sengoda, V. G.; Buchman, J. L.; Fisher, T. W.] Washington State Univ, Wapato, WA USA. [Sengoda, V. G.; Munyaneza, J. E.; Buchman, J. L.; Fisher, T. W.] USDA ARS, Wapato, WA USA. [Pappu, H. R.] Washington State Univ, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S162 EP S162 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401366 ER PT J AU Serrato-Diaz, LM Rivera-Vargas, LI Goenaga, RJ French-Monar, RD AF Serrato-Diaz, L. M. Rivera-Vargas, L. I. Goenaga, R. J. French-Monar, R. D. TI Pathogenic and non-pathogenic fungi associated with longan (Dimocarpus longan L.) in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Serrato-Diaz, L. M.; French-Monar, R. D.] AgriLife Extens Texas A&M, Amarillo, TX USA. [Rivera-Vargas, L. I.] Univ Puerto Rico, Mayaguez, PR USA. [Goenaga, R. J.] ARS, USDA, Trop Agr Res Stn, Mayaguez, PR USA. [Goenaga, R. J.] Univ Puerto Rico Mayaguez Campus, Mayaguez, PR USA. NR 0 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S163 EP S163 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401370 ER PT J AU Shaner, D AF Shaner, D. TI The current state of resistance to acetohydroxyacid/acetolactate synthase inhibitors SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Shaner, D.] ARS, USDA, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S236 EP S236 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401795 ER PT J AU Sharma-Poudyal, D Chen, X AF Sharma-Poudyal, D. Chen, X. TI Virulence diversity of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp tritici SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Chen, X.] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S164 EP S164 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401378 ER PT J AU Sheng, H See, DR Murray, TD AF Sheng, H. See, D. R. Murray, T. D. TI QTL mapping of resistance genes for eyespot of wheat in Aegilops longissima SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [See, D. R.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S165 EP S165 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401380 ER PT J AU Shishkoff, N AF Shishkoff, N. TI Risk analysis of native and ornamental plants for root infection and inoculum production from roots by Phytophthora ramorum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Shishkoff, N.] ARS, USDA, FDWSRU, Frederick, MD USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S166 EP S166 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401386 ER PT J AU Smith, BJ AF Smith, B. J. TI Effect of bed height and soil amendments on survival of southern highbush blueberry cultivars in Phytophthora spp. infested soils in Mississippi SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Smith, B. J.] ARS, USDA, So Hort Lab, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S167 EP S167 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401396 ER PT J AU Sparks, AN Gadal, L Ni, X AF Sparks, A. N. Gadal, L. Ni, X. TI Efficacy of silk channel injections with insecticides for management of lepidopteran pests of sweet corn SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sparks, A. N.] Univ Georgia Cooperat Extens, Tifton, GA USA. [Gadal, L.] Monsanto Dekalb, Montpellier, France. [Ni, X.] USDA ARS, Crops Genet & Breeding Res Unit, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S169 EP S170 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401409 ER PT J AU Stenger, DC Sisterson, MS French, R AF Stenger, D. C. Sisterson, M. S. French, R. TI Homalodisca vitripennis reovirus polymorphism validates timing and limited introduction of glassy-winged sharpshooter to California SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Stenger, D. C.; Sisterson, M. S.] USDA ARS, Parlier, CA USA. [French, R.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S171 EP S171 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401418 ER PT J AU Stewart, JE Abdo, Z Dumroese, R Klopfenstein, NB Kim, M AF Stewart, J. E. Abdo, Z. Dumroese, R. Klopfenstein, N. B. Kim, M. TI Virulence of Fusarium root-disease pathogens (Fusarium oxysporum and F. commune) to Douglas-fir (Pseudotsuga menziesii) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Stewart, J. E.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Abdo, Z.] Univ Idaho, Dept Math, Moscow, ID 83843 USA. [Abdo, Z.] Univ Idaho, Dept Stat, Moscow, ID 83843 USA. [Dumroese, R.; Klopfenstein, N. B.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID USA. [Kim, M.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S171 EP S171 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401420 ER PT J AU Stobbe, AH Daniels, J Espindola, A Schneider, WL Fletcher, J Melcher, UK AF Stobbe, A. H. Daniels, J. Espindola, A. Schneider, W. L. Fletcher, J. Melcher, U. K. TI Use of massively parallel sequencing as a diagnostic tool SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Stobbe, A. H.; Daniels, J.; Espindola, A.; Melcher, U. K.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W. L.] ARS, Foreign Dis Weed Sci Res Unit, USDA, Ft Detrick, MD USA. [Fletcher, J.] NIMFFAB, Stillwater, OK USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S171 EP S172 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401421 ER PT J AU Stockwell, VO Shaffer, BT Henkels, MD Pscheidt, JW Loper, JE AF Stockwell, V. O. Shaffer, B. T. Henkels, M. D. Pscheidt, J. W. Loper, J. E. TI Factors influencing efficacy of plastic shelters for control of bacterial blight of lilac SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Stockwell, V. O.; Pscheidt, J. W.] Oregon State Univ, Corvallis, OR 97331 USA. [Shaffer, B. T.; Henkels, M. D.; Loper, J. E.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S172 EP S172 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401422 ER PT J AU Strausbaugh, CA Rearick, E Eujayl, IA Foote, P AF Strausbaugh, C. A. Rearick, E. Eujayl, I. A. Foote, P. TI Influence of rhizoctonia-bacterial root rot complex on storability of sugar beet SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Strausbaugh, C. A.] USDA ARS NWISRL, Kimberly, ID USA. [Rearick, E.] Amalgamated Res LLC, Twin Falls, ID USA. [Eujayl, I. A.] ARS, USDA, Kimberly, ID USA. [Foote, P.] Amalgamated Sugar Co LLC, Paul, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S172 EP S172 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401424 ER PT J AU Sudarshana, MR Gonzalez, A Dave, A Uyemoto, JK AF Sudarshana, M. R. Gonzalez, A. Dave, A. Uyemoto, J. K. TI A quantitative PCR assay for the detection of phytoplasmas causing almond brownline, peach yellow leafroll, and pear decline diseases in California SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Sudarshana, M. R.; Gonzalez, A.] ARS, USDA, Davis, CA USA. [Dave, A.] UC Davis, Davis, CA USA. [Uyemoto, J. K.] Univ Calif Davis, ARS, USDA, Davis, CA 95616 USA. NR 0 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S172 EP S173 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401427 ER PT J AU Thies, JA Ariss, JJ Kousik, CS AF Thies, J. A. Ariss, J. J. Kousik, C. S. TI Response of pepper (Capsicum annuum) genotypes to co-infection by Phytophthora capsici and Meloidogyne incognita SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Thies, J. A.; Ariss, J. J.; Kousik, C. S.] ARS, US Vegetable Lab, USDA, Charleston, SC USA. NR 0 TC 2 Z9 2 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S176 EP S176 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401447 ER PT J AU Thomashow, LS Mavrodi, DV Hassan, KA Paulsen, IT Loper, JE Alfano, JR Weller, DM AF Thomashow, L. S. Mavrodi, D. V. Hassan, K. A. Paulsen, I. T. Loper, J. E. Alfano, J. R. Weller, D. M. TI Novel pathways revealed in P. fluorescens Q2-87 and Q8r1-96 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Thomashow, L. S.; Weller, D. M.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Mavrodi, D. V.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Hassan, K. A.; Paulsen, I. T.] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. [Loper, J. E.] USDA ARS, Corvallis, OR USA. [Alfano, J. R.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE USA. RI Hassan, Karl/B-2802-2013; Mavrodi, Dmitri/J-2745-2013 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S231 EP S231 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401764 ER PT J AU Tian, B Schneider, WL Gildow, FE AF Tian, B. Schneider, W. L. Gildow, F. E. TI Tradeoffs between host adaptation and vector transmission of Soybean Dwarf Virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Tian, B.; Gildow, F. E.] Penn State Univ, Dept Plant Pathol, State Coll, PA USA. [Schneider, W. L.] ARS, Foreign Dis Weed Sci Res Unit, USDA, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S176 EP S176 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401450 ER PT J AU Tjosvold, S Chambers, D Mori, S AF Tjosvold, S. Chambers, D. Mori, S. TI Effect of environmental conditions and lesion age on sporulation of Phytophthora ramorum on California bay, rhododendron, and camellia SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Tjosvold, S.; Chambers, D.] Univ Calif Cooperat Extens, Watsonville, CA USA. [Mori, S.] US Forest Serv, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S177 EP S177 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401452 ER PT J AU Tooley, PW Browning, M AF Tooley, P. W. Browning, M. TI Inoculum density effects on infection of selected Eastern US forest species by Phytophthora ramorum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Tooley, P. W.; Browning, M.] USDA ARS, FDWSRU, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S274 EP S275 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402054 ER PT J AU Travadon, R Fujiyoshi, P Smith, ME Douhan, GW Rizzo, DM Baumgartner, K AF Travadon, R. Fujiyoshi, P. Smith, M. E. Douhan, G. W. Rizzo, D. M. Baumgartner, K. TI Clonal and sexual dispersal of Armillaria mellea in an ornamental landscape SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Travadon, R.; Rizzo, D. M.] Univ Calif Davis, Davis, CA 95616 USA. [Fujiyoshi, P.; Baumgartner, K.] ARS, USDA, Davis, CA USA. [Smith, M. E.] Duke Univ, Durham, NC USA. [Douhan, G. W.] Univ Calif Riverside, Riverside, CA 92521 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S178 EP S178 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401459 ER PT J AU Tremblay, A Hosseini, P Li, S Alkharouf, NW Matthews, BF AF Tremblay, A. Hosseini, P. Li, S. Alkharouf, N. W. Matthews, B. F. TI Discovery of new soybean and soybean rust genes using next generation sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Tremblay, A.; Hosseini, P.; Matthews, B. F.] ARS, USDA, PSI, SGIL, Beltsville, MD USA. [Li, S.] ARS, USDA, CGRU, Stoneville, MS USA. [Alkharouf, N. W.] Towson Univ, Towson, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S209 EP S209 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401636 ER PT J AU Vaira, A Lim, H Owens, RA Dienelt, MM Reinsel, MD Hammond, J AF Vaira, A. Lim, H. Owens, R. A. Dienelt, M. M. Reinsel, M. D. Hammond, J. TI Production of both carboxy-coterminal coat protein forms of Lolium latent virus is required for efficient systemic movement SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Lim, H.] CNR, Ist Virol Vegetale, I-10126 Turin, Italy. [Owens, R. A.] Chungnam Natl Univ, Taejon, South Korea. [Dienelt, M. M.] ARS, USDA, MPPL, Beltsville, MD USA. [Hammond, J.] ARS, USDA, FNPRU, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S181 EP S181 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401475 ER PT J AU van Waes, JG Maddox, J Ashley-Koch, A Gregory, S de Matute, OT Voss, KA Riley, RT AF van Waes, J. Gelineau Maddox, J. Ashley-Koch, A. Gregory, S. Torres de Matute, O. Voss, K. A. Riley, R. T. TI Evaluating human exposure to fumonisins in Guatemala and its possible role as a contributing factor to neural tube defects SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [van Waes, J. Gelineau; Maddox, J.] Creighton Univ, Omaha, NE 68178 USA. [Ashley-Koch, A.; Gregory, S.] Duke Univ, Med Ctr, Durham, NC USA. [Torres de Matute, O.] Ctr Invest Nutr & Salud, Guatemala City, Guatemala. [Voss, K. A.; Riley, R. T.] ARS, USDA, Toxicol & Mycotoxin Res Unit, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S222 EP S222 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401713 ER PT J AU Cruz, CMV Balidion, JF Choi, I Mauleon, RP Satoh, K Kikuchi, S Goodwin, PH Webb, KM Garrett, KA Wydra, K Leach, JE AF Vera Cruz, C. M. Balidion, J. F. Choi, I. Mauleon, R. P. Satoh, K. Kikuchi, S. Goodwin, P. H. Webb, K. M. Garrett, K. A. Wydra, K. Leach, J. E. TI Inverse responses of two major genes against bacterial blight of rice at different temperature regimes SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Vera Cruz, C. M.] Intl Rice Res Inst, Manila, Philippines. [Balidion, J. F.] Univ Philippines, Coll Agr, Los Banos, Philippines. [Choi, I.] Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, Los Banos, Philippines. [Mauleon, R. P.] Int Rice Res Inst, TT Chang Genet Resources Ctr, Los Banos, Philippines. [Satoh, K.; Kikuchi, S.] Natl Inst Agrobiol Sci, Div Genome & Biodivers Res, Plant Genome Res Unit, Tsukuba, Ibaraki, Japan. [Goodwin, P. H.] Univ Guelph, Ontario Agr Coll, Dept Environm Biol, Guelph, ON N1G 2W1, Canada. [Webb, K. M.] ARS, USDA, Sugar Beet Res Unit, Ft Collins, CO USA. [Garrett, K. A.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Wydra, K.] Georg August Univ Goettingen, Tropenzentrum, Ctr Trop & Subtrop Agr & Forestry, Gottingen, Germany. [Leach, J. E.] Colorado State Univ, Ft Collins, CO 80523 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S182 EP S183 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401486 ER PT J AU Vico, I Jurick, WM Gaskins, VL Peter, KA Janisiewicz, WJ Conway, WS AF Vico, I. Jurick, W. M., II Gaskins, V. L. Peter, K. A. Janisiewicz, W. J. Conway, W. S. TI Nutritional cues and ambient pH modulate the in vitro activity of a polygalacturonase isozyme produced by Penicillium expansum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Vico, I.] ARS, USDA, Food Qual Lab, Beltsville, MD USA. [Jurick, W. M., II; Gaskins, V. L.; Peter, K. A.; Conway, W. S.] ARS, USDA, Beltsville, MD USA. [Janisiewicz, W. J.] ARS, USDA, Kerneysville, WV USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S183 EP S183 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401489 ER PT J AU Wallis, C Sisterson, MS AF Wallis, C. Sisterson, M. S. TI Xylella fastidiosa infection of grapevines affects host secondary metabolite and defense-related protein levels within xylem SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wallis, C.; Sisterson, M. S.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S185 EP S185 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401499 ER PT J AU Wallis, C Chen, J AF Wallis, C. Chen, J. TI Zebra chip disease is associated with increases in pathogenesis-related protein activity and host defense-associated secondary metabolites in tubers SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Chen, J.] ARS, USDA, PWA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S184 EP S185 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401498 ER PT J AU Wallis, C Krugner, R Walse, S AF Wallis, C. Krugner, R. Walse, S. TI Grape hosts infested with glassy-winged sharpshooters produce volatile compounds which may attract egg parasitoids SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wallis, C.; Krugner, R.; Walse, S.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S184 EP S184 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401497 ER PT J AU Walters, TW AF Walters, T. W. TI Designing, developing, and delivering digital identification tools for plant protection and quarantine SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Walters, T. W.] USDA APHIS PPQ CPHST, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S218 EP S218 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401690 ER PT J AU Wang, C Mullens, TR Ulloa, M Roberts, PA AF Wang, C. Mullens, T. R. Ulloa, M. Roberts, P. A. TI QTL analysis for transgressive resistance to root-knot nematode in a cotton RIL population derived from interspecific susceptible parents (Gossypium spp.) SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wang, C.; Mullens, T. R.; Roberts, P. A.] Univ Calif Riverside, Riverside, CA 92521 USA. [Ulloa, M.] ARS, USDA, Western Intergrated Cropping Syst Res Unit, Shafter, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S187 EP S187 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401513 ER PT J AU Wang, D Calla, B Qi, M Korban, S Clough, S Sundin, G Toth, I Zhao, Y AF Wang, D. Calla, B. Qi, M. Korban, S. Clough, S. Sundin, G. Toth, I. Zhao, Y. TI Genome-wide identification of genes regulated by RcsB and RcsC in Erwinia amylovora SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wang, D.; Calla, B.; Qi, M.; Korban, S.; Zhao, Y.] Univ Illinois, Urbana, IL 61801 USA. [Clough, S.] ARS, USDA, Urbana, IL USA. [Sundin, G.] Michigan State Univ, E Lansing, MI 48824 USA. [Toth, I.] SCRI, Plant Pathol Program, Dundee, Scotland. RI Wang, Dongping /L-2124-2013 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S186 EP S186 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401505 ER PT J AU Wang, K Cabos, R AF Wang, K. Cabos, R. TI Food and microhabitat preferences of Mononchus: A preliminary investigation SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wang, K.] Univ Hawaii, Honolulu, HI 96822 USA. [Cabos, R.] ARS, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S185 EP S186 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401504 ER PT J AU Wanner, LA Qu, X Christ, BJ AF Wanner, L. A. Qu, X. Christ, B. J. TI A common scab resistant potato cultivar is not explained by pathogen growth in soil or window of infectivity SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wanner, L. A.] ARS, USDA, Beltsville, MD USA. [Qu, X.; Christ, B. J.] Penn State Univ, University Pk, PA 16802 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S188 EP S188 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401518 ER PT J AU Webster, CG Reitz, SR Frantz, G Mellinger, H Perry, KL Adkins, S AF Webster, C. G. Reitz, S. R. Frantz, G. Mellinger, H. Perry, K. L. Adkins, S. TI A novel M RNA reassortant of Groundnut ringspot virus and Tomato chlorotic spot virus infecting vegetables in Florida SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Webster, C. G.; Adkins, S.] ARS, USDA, Ft Pierce, FL USA. [Reitz, S. R.] ARS, USDA, Tallahassee, FL USA. [Frantz, G.; Mellinger, H.] Glades Crop Care Inc, Jupiter, FL USA. [Perry, K. L.] Cornell Univ, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S189 EP S189 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401524 ER PT J AU Wechter, W Keinath, A Smith, P Farnham, M AF Wechter, W. Keinath, A. Smith, P. Farnham, M. TI Evaluation of leaf blight-resistant plant introductions of Brassica juncea and Brassica rapa and elucidation of inheritance of resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wechter, W.; Farnham, M.] ARS, USDA, Charleston, SC USA. [Keinath, A.] Clemson Univ, Charleston, SC USA. [Smith, P.] Clemson Univ, Lexington, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S189 EP S189 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401525 ER PT J AU Weems, JD Zhang, GR Ames, KA Haudenshield, JS Hartman, GL Bond, JP Bradley, CA AF Weems, J. D. Zhang, G. R. Ames, K. A. Haudenshield, J. S. Hartman, G. L. Bond, J. P. Bradley, C. A. TI Effect of fungicide seed treatments on Fusarium virguliforme and Sudden death syndrome of soybean SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Bond, J. P.] So Illinois Univ, Carbondale, IL 62901 USA. [Haudenshield, J. S.; Hartman, G. L.] USDA, Washington, DC USA. [Weems, J. D.; Zhang, G. R.; Ames, K. A.; Bradley, C. A.] Univ Illinois, Chicago, IL 60680 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S246 EP S246 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401846 ER PT J AU Wen, A Lin, H Gudmestad, NC AF Wen, A. Lin, H. Gudmestad, N. C. TI Development of PCR assay using simple sequence repeat primers for detection of 'Candidatus Liberibacter solanacearum' SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wen, A.; Gudmestad, N. C.] N Dakota State Univ, Fargo, ND 58105 USA. [Lin, H.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S190 EP S190 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401529 ER PT J AU Whitworth, JL Novy, RG Yilma, S AF Whitworth, J. L. Novy, R. G. Yilma, S. TI Potato virus Y resistance from Ry(adg) and Ry(sto) genes: Practical application in a potato breeding program SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT American Phytopathological Society (APS)/International Plant Protection Congress(IPPS) Joint Meeting CY AUG 06-10, 2011 CL Honolulu, HI SP Amer Phytopathol Soc (APS), Int Plant Protection Congress (IPPS) C1 [Whitworth, J. L.; Novy, R. G.] ARS, USDA, Aberdeen, ID USA. [Yilma, S.] Oregon State Univ, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S190 EP S190 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401533 ER PT J AU Widmer, TL AF Widmer, T. L. TI Sporulation potential of Phytophthora kernoviae compared to P. syringae and P. cactorum on selected hosts SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Widmer, T. L.] USDA ARS FDWSRU, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S191 EP S191 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401535 ER PT J AU Widmer, TL Johnson-Brousseau, SA Ghosh, S AF Widmer, T. L. Johnson-Brousseau, S. A. Ghosh, S. TI Management of Phytophthora ramorum-infested nursery soil with Trichoderma asperellum SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Widmer, T. L.] USDA ARS FDWSRU, Frederick, MD USA. [Johnson-Brousseau, S. A.; Ghosh, S.] Dominican Univ Calif, San Rafael, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S191 EP S191 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401536 ER PT J AU Wilson, A Oberle, CS AF Wilson, A. Oberle, C. S. TI Development of an electronic-nose technology for the rapid detection and discrimination of subterranean termites within wood in service SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wilson, A.; Oberle, C. S.] US Forest Serv, USDA, Stoneville, MS USA. NR 0 TC 1 Z9 1 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S192 EP S192 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401541 ER PT J AU Wintermantel, WM AF Wintermantel, W. M. TI Differential effects of host plants on accumulation, competition and transmission of curtoviruses from single and mixed infections SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wintermantel, W. M.] ARS, USDA, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S192 EP S192 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401544 ER PT J AU Wu, W Ding, Y Wei, W Lee, I Davis, RE Zhao, Y AF Wu, W. Ding, Y. Wei, W. Lee, I. Davis, R. E. Zhao, Y. TI Gibberellin acid (GA3) treatment attenuates tomato floral deformation caused by potato purple top phytoplasma infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Wu, W.; Ding, Y.; Wei, W.; Lee, I.; Davis, R. E.; Zhao, Y.] ARS, MPPL, USDA, Beltsville, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S275 EP S275 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402056 ER PT J AU Xu, D Mock, R Kinard, G Li, R AF Xu, D. Mock, R. Kinard, G. Li, R. TI Molecular analysis of complete genomic sequences of four isolates of Gooseberry vein banding associated virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Xu, D.; Mock, R.; Kinard, G.; Li, R.] USDA ARS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S195 EP S195 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401558 ER PT J AU Xu, D Liu, H Li, F Howell, B Tian, T Li, R AF Xu, D. Liu, H. Li, F. Howell, B. Tian, T. Li, R. TI Biological characterization and complete genomic sequence of Carrot thin leaf virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Xu, D.; Li, F.; Li, R.] USDA ARS, Beltsville, MD USA. [Liu, H.] USDA ARS, Salinas, CA USA. [Howell, B.] Washington State Univ, Prosser, WA USA. [Tian, T.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S195 EP S195 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401559 ER PT J AU Yakabe, LE Parker, SR AF Yakabe, L. E. Parker, S. R. TI Comparison of seed health methods for the detection of Acidovorax avenae subsp citrulli in cucurbit seeds SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Yakabe, L. E.] USDA ARS, CPGRU, Davis, CA USA. [Parker, S. R.] Syngenta, Nampa, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S196 EP S196 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401567 ER PT J AU Yakabe, LE Parker, SR Kluepfel, DA AF Yakabe, L. E. Parker, S. R. Kluepfel, D. A. TI Evaluations and modifications of semi-selective media for improved isolation of Agrobacterium tumefaciens biovar 1 from cultivated walnut SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Yakabe, L. E.; Parker, S. R.; Kluepfel, D. A.] ARS, USDA, Crops Pathol Genet Res Unit, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S196 EP S196 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401566 ER PT J AU Yan, G Smiley, RW Okubara, PA AF Yan, G. Smiley, R. W. Okubara, P. A. TI Development of a species-specific PCR assay to identify the cereal cyst nematode Heterodera filipjevi SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Yan, G.; Smiley, R. W.] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR USA. [Okubara, P. A.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S196 EP S196 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401569 ER PT J AU Yang, H Hartman, GL AF Yang, H. Hartman, G. L. TI Identification and pathogenic analysis of Colletotrichum species causing soybean anthracnose SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT American Phytopathological Society (APS)/International Plant Protection Congress(IPPS) Joint Meeting CY AUG 06-10, 2011 CL Honolulu, HI SP Amer Phytopathol Soc (APS), Int Plant Protection Congress (IPPS) C1 [Yang, H.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Dept Crop Sci, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S197 EP S197 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401573 ER PT J AU Yuan, Q Jordan, R Brlansky, RH Minenkova, O Hartung, J AF Yuan, Q. Jordan, R. Brlansky, R. H. Minenkova, O. Hartung, J. TI Production of single chain antibodies against 'Ca. Liberibacter asiaticus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Yuan, Q.; Hartung, J.] USDA ARS MPPL, Beltsville, MD USA. [Jordan, R.] USDA ARS FNPRU, Beltsville, MD USA. [Brlansky, R. H.] Univ Florida, Lake Alfred, FL USA. [Minenkova, O.] Sigma Tau Pharmaceut, Rome, Italy. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S199 EP S199 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401587 ER PT J AU Zapata, M Hartung, J Brodbeck, B Andersen, P AF Zapata, M. Hartung, J. Brodbeck, B. Andersen, P. TI Endophytic bacteria from the vascular tissue of coffee (Coffea arabica L.) and citrus (Citrus sinensis L.) leaves found during the attempt to isolate the pathogen, Xylella fastidiosa in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zapata, M.] Univ Puerto Rico, Dept Crops & Agroenvironm Sci, Mayaguez, PR USA. [Hartung, J.] USDA ARS, Mol Plant Pathol Lab, Beltsville Agr Res Ctr W, Beltsville, MD USA. [Brodbeck, B.; Andersen, P.] Univ Florida, N Florida Res & Educ Ctr Quincy, Quincy, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S279 EP S279 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402073 ER PT J AU Zasada, I AF Zasada, I. TI Viruses transmitted by nematodes: When the germs meet the worms SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT American Phytopathological Society (APS)/International Plant Protection Congress(IPPS) Joint Meeting CY AUG 06-10, 2011 CL Honolulu, HI SP Amer Phytopathol Soc (APS), Int Plant Protection Congress (IPPS) C1 [Zasada, I.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S209 EP S209 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401639 ER PT J AU Zerillo, MM Woeste, K Freeland, E Seybold, S Cranshaw, W Tisserat, N AF Zerillo, M. M. Woeste, K. Freeland, E. Seybold, S. Cranshaw, W. Tisserat, N. TI Population structure of Geosmithia morbida in the United States is complex SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zerillo, M. M.; Freeland, E.; Cranshaw, W.; Tisserat, N.] Colorado State Univ, Ft Collins, CO 80523 USA. [Woeste, K.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Seybold, S.] US Forest Serv, USDA, Davis, CA USA. RI zerillo, marcelo/M-4159-2016 OI zerillo, marcelo/0000-0001-9671-2894 NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S201 EP S201 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401596 ER PT J AU Zhan, G Chen, X Kang, Z Wang, M Wan, A Cheng, P AF Zhan, G. Chen, X. Kang, Z. Wang, M. Wan, A. Cheng, P. TI Virulence and molecular comparison of Puccinia striiformis f. sp tritici populations in China and the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhan, G.] NW A&F Univ, Coll Crop Protect, Yangling, Shaanxi, Peoples R China. [Kang, Z.] NW A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Wang, M.; Wan, A.; Cheng, P.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RI Zhao, jing/B-7349-2008; WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S201 EP S201 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401597 ER PT J AU Zhang, J Thomashow, LS Mavrodi, OV Weller, DM Mavrodi, DV AF Zhang, J. Thomashow, L. S. Mavrodi, O. V. Weller, D. M. Mavrodi, D. V. TI Construction of recombinant fluorescent Pseudomonas spp. for suppression of soilborne pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhang, J.] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Peoples R China. [Thomashow, L. S.; Weller, D. M.] ARS, USDA, Pullman, WA USA. [Mavrodi, O. V.] Washington State Univ, Pullman, WA 99164 USA. RI Mavrodi, Dmitri/J-2745-2013 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S201 EP S201 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401598 ER PT J AU Zhang, M Duan, Y Powell, C AF Zhang, M. Duan, Y. Powell, C. TI Evaluation of chemicals for control of citrus canker, Xanthomonas citri subsp citri SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Powell, C.] Univ Florida, IRREC IFAS, Ft Pierce, FL USA. [Duan, Y.] ARS, USHRL, USDA, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S202 EP S202 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401601 ER PT J AU Zhang, R Hartman, GL Eastburn, DM AF Zhang, R. Hartman, G. L. Eastburn, D. M. TI Interactions of post emergence herbicides, strobulurin fungicides, and Rhizoctonia root rot of soybean SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Hartman, G. L.] Univ Illinois, USDA, ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S203 EP S203 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401606 ER PT J AU Zhou, J Karen, K Robert, M Tzanetakis, I AF Zhou, J. Karen, K. Robert, M. Tzanetakis, I. TI Sweet bunden of sugarberry - a novel ampelovirus found in Celtis laevigata SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, J.; Tzanetakis, I.] Univ Arkansas, Dept Plant Pathol, Div Agr & Cell, Fayetteville, AR 72701 USA. [Zhou, J.; Tzanetakis, I.] Univ Arkansas, Program Mol Biol, Fayetteville, AR 72701 USA. [Karen, K.; Robert, M.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S206 EP S206 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401624 ER PT J AU Zhou, L Armstrong, C Powell, CA Duan, Y AF Zhou, L. Armstrong, C. Powell, C. A. Duan, Y. TI Metagenomic analysis of Candidatus Liberibacter asiaticus in naturally populated psyllids (Diaphorina citri) using BAC libraries SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, L.] Hort Res Lab, Ft Pierce, FL USA. [Armstrong, C.; Duan, Y.] ARS, USDA, USHRL, Ft Pierce, FL USA. [Powell, C. A.] Univ Florida, IFAS IRREC, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S205 EP S206 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401623 ER PT J AU Zhou, X Ren, R Wang, M Chen, X AF Zhou, X. Ren, R. Wang, M. Chen, X. TI Molecular mapping of new genes for stripe rust resistance in spring wheat genotypes PI 178759 and PI 183527 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, X.; Ren, R.; Wang, M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X.] ARS, USDA, Pullman, WA USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S205 EP S205 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401622 ER PT J AU Zhou, XG McClung, AM AF Zhou, X. G. McClung, A. M. TI Disease severity and yield potential of rice cultivars in organic production systems SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, X. G.] Texas A&M Univ Syst, AgriLife Res & Extens Ctr, Beaumont, TX USA. [McClung, A. M.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S270 EP S271 PG 2 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045402038 ER PT J AU Zhou, XG McClung, AM Way, MO Jo, Y Tabien, RE Wilson, LT AF Zhou, X. G. McClung, A. M. Way, M. O. Jo, Y. Tabien, R. E. Wilson, L. T. TI Severe outbreak of bacterial panicle blight across Texas Rice Belt in 2010 SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, X. G.; Way, M. O.; Tabien, R. E.; Wilson, L. T.] Texas A&M Univ, Beaumont, TX USA. [McClung, A. M.] ARS, USDA, Rice Res Unit, Beaumont, TX USA. [Jo, Y.] Texas A&M Univ, College Stn, TX USA. RI Wilson, Lloyd/E-9971-2011 NR 0 TC 0 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S205 EP S205 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401618 ER PT J AU Zhou, XG Tabien, RE McClung, AM AF Zhou, X. G. Tabien, R. E. McClung, A. M. TI Field disease reaction of rice cultivars and elite lines in Texas SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zhou, X. G.; Tabien, R. E.] Texas A&M Univ Syst, Beaumont, TX USA. [McClung, A. M.] ARS, USDA, Rice Res Unit, Beaumont, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S204 EP S204 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401615 ER PT J AU Ziska, LH AF Ziska, L. H. TI Climate change and plant protection: Emerging viral and weed threats SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Ziska, L. H.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S240 EP S240 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401814 ER PT J AU Zitomer, NC Baldwin, TT Glenn, AE Bacon, CW Riley, RT AF Zitomer, N. C. Baldwin, T. T. Glenn, A. E. Bacon, C. W. Riley, R. T. TI Fusarium verticillioides infection of maize seedlings and the corresponding movement of fungus, fumonisins, and biomarkers of exposure SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Glenn, A. E.] ARS, USDA, Richard B Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S206 EP S206 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401625 ER PT J AU Zou, H Duan, Y Gowda, S Powell, CA Chen, G AF Zou, H. Duan, Y. Gowda, S. Powell, C. A. Chen, G. TI Characterization of the host defense response induced by the flagellin protein of Candidatus Liberibacter asiaticus SO PHYTOPATHOLOGY LA English DT Meeting Abstract C1 [Zou, H.; Gowda, S.] Univ Florida, Citrus Res & Educ Ctr, IFAS, Lake Alfred, FL 33805 USA. [Duan, Y.] ARS, USDA, USHRL, Ft Pierce, FL USA. [Powell, C. A.] Univ Florida, IFAS, IRREC, Ft Pierce, FL USA. [Chen, G.] Shanghai Jiao Tong Univ, Dept Environm Resources, Shanghai 200030, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 SU S BP S206 EP S206 PG 1 WC Plant Sciences SC Plant Sciences GA 822KR UT WOS:000295045401627 ER PT J AU Wenger, SJ Isaak, DJ Dunham, JB Fausch, KD Luce, CH Neville, HM Rieman, BE Young, MK Nagel, DE Horan, DL Chandler, GL AF Wenger, Seth J. Isaak, Daniel J. Dunham, Jason B. Fausch, Kurt D. Luce, Charles H. Neville, Helen M. Rieman, Bruce E. Young, Michael K. Nagel, David E. Horan, Dona L. Chandler, Gwynne L. TI Role of climate and invasive species in structuring trout distributions in the interior Columbia River Basin, USA SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID WESTERN UNITED-STATES; SUMMER STREAM TEMPERATURES; WESTSLOPE CUTTHROAT TROUT; ROCKY-MOUNTAIN STREAMS; BROOK TROUT; BULL TROUT; SALMO-TRUTTA; BROWN TROUT; SALVELINUS-CONFLUENTUS; AIR-TEMPERATURE AB Recent and projected climate warming trends have prompted interest in impacts on coldwater fishes. We examined the role of climate (temperature and flow regime) relative to geomorphology and land use in determining the observed distributions of three trout species in the interior Columbia River Basin, USA. We considered two native species, cutthroat trout (Oncorhynchus clarkii) and bull trout (Salvelinus confluentus), as well as nonnative brook trout (Salvelinus fontinalis). We also examined the response of the native species to the presence of brook trout. Analyses were conducted using multilevel logistic regression applied to a geographically broad database of 4165 fish surveys. The results indicated that bull trout distributions were strongly related to climatic factors, and more weakly related to the presence of brook trout and geomorphic variables. Cutthroat trout distributions were weakly related to climate but strongly related to the presence of brook trout. Brook trout distributions were related to both climate and geomorphic variables, including proximity to unconfined valley bottoms. We conclude that brook trout and bull trout are likely to be adversely affected by climate warming, whereas cutthroat trout may be less sensitive. The results illustrate the importance of considering species interactions and flow regime alongside temperature in understanding climate effects on fish. C1 [Wenger, Seth J.; Neville, Helen M.] Trout Unltd, Boise, ID 83702 USA. [Isaak, Daniel J.; Luce, Charles H.; Nagel, David E.; Horan, Dona L.; Chandler, Gwynne L.] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [Fausch, Kurt D.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Rieman, Bruce E.] US Forest Serv, Rocky Mt Res Stn, Seeley Lake, MT 59868 USA. [Young, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Wenger, SJ (reprint author), Trout Unltd, 322 E Front St,Suite 401, Boise, ID 83702 USA. EM swenger@tu.org RI Wenger, Seth/G-6594-2011; Luce, Charles/A-9267-2008; Fausch, Kurt/A-8849-2010 OI Luce, Charles/0000-0002-6938-9662; FU National Fish and Wildlife Foundation's Keystone Initiative for Freshwater Fishes [2008-0087-000] FX This work was funded by grant 2008-0087-000 of the National Fish and Wildlife Foundation's Keystone Initiative for Freshwater Fishes. The fish data used in this study were compiled from multiple sources, including a previous database of sites in the range of westslope cutthroat trout (Rieman et al. 1999), which included data from the Idaho Fish and Game's General Parr Monitoring database and other sources. Additional data used in this study were provided by Bart Gammett of the Salmon-Challis National Forest; James Capurso of the Caribou-Targhee National Forest; Mark Novak of the Bridger-Teton National Forest; Steven Kujala of the Beaverhead-Deerlodge National Forest; Joseph Benjamin, formerly of the U.S.D.A. Forest Service Rocky Mountain Research Station; Hilda Sexauer of Wyoming Game and Fish; Kevin Meyer of Idaho Fish and Game; and Brad Shepard of Montana Fish, Wildlife and Parks. Sharon Parkes of the U.S.D.A. Forest Service Rocky Mountain Research Station (RMRS) helped to develop the GIS coverages used in this analysis. Expert advice over the course of the study was provided by Russ Thurow, John Buffington, and Jim McKean of RMRS; Doug Peterson of the US Fish and Wildlife Service; Dan Dauwalter and Jack Williams of Trout Unlimited; Brad Shepard of Montana Fish, Wildlife and Parks; and Colden Baxter of Idaho State University. Brian Cade of US Geological Survey provided statistical advice. Alan Hamlet and Marketa McGuire Elsner of the Climate Impacts Group performed the VIC hydrologic modeling used in this study. This paper was substantially improved by reviews from Brad Shepard, Dan Dauwalter, and three anonymous reviewers. Use of trade or firm names in this manuscript is for reader information only and does not imply endorsement of any product or service by the US government. NR 108 TC 36 Z9 36 U1 9 U2 58 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD JUN PY 2011 VL 68 IS 6 BP 988 EP 1008 DI 10.1139/F2011-034 PG 21 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 813MF UT WOS:000294370600004 ER PT J AU Morris, MS Jacques, PF Chylack, LT Hankinson, SE Willett, WC Taylor, A AF Morris, M. S. Jacques, P. F. Chylack, L. T., Jr. Hankinson, S. E. Willett, W. C. Taylor, A. TI SUPPLEMENTAL VITAMINS AND VISUAL ACUITY IN THE PRESENCE OF EARLY AGE-RELATED OCULAR LESIONS SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Meeting Abstract CT 3rd North American Congress of Epidemiology CY JUN 21-24, 2011 CL Montreal, CANADA C1 [Morris, M. S.; Jacques, P. F.; Chylack, L. T., Jr.; Hankinson, S. E.; Willett, W. C.; Taylor, A.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 1 PY 2011 VL 173 SU 11 BP S60 EP S60 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 810GU UT WOS:000294114600234 ER PT J AU Nord, M AF Nord, M. TI WHY IS HOUSEHOLD FOOD SECURITY BETTER IN CANADA THAN IN THE UNITED STATES? SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Meeting Abstract CT 3rd North American Congress of Epidemiology CY JUN 21-24, 2011 CL Montreal, CANADA C1 [Nord, M.] Econ Res Serv, Food Assistance Branch, USDA, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 1 PY 2011 VL 173 SU 11 BP S241 EP S241 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 810GU UT WOS:000294114601235 ER PT J AU Nord, M AF Nord, Mark TI INTRA-HOUSEHOLD DIFFERENCES IN FOOD SECURITY AMONG ADULTS IN NHANES SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Meeting Abstract CT 3rd North American Congress of Epidemiology CY JUN 21-24, 2011 CL Montreal, CANADA C1 [Nord, Mark] USDA, Econ Res Serv, Washington, DC USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 1 PY 2011 VL 173 SU 11 BP S326 EP S326 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 810GU UT WOS:000294114601565 ER PT J AU Chen, S Fleischer, SJ Tobin, PC Saunders, MC AF Chen, Shi Fleischer, Shelby J. Tobin, Patrick C. Saunders, Michael C. TI Projecting Insect Voltinism Under High and Low Greenhouse Gas Emission Conditions SO ENVIRONMENTAL ENTOMOLOGY LA English DT Review DE greenhouse gas emissions; climate change; phenology; voltinism; grape berry moth ID BERRY MOTH LEPIDOPTERA; CLIMATE-CHANGE; POPULATION-DYNAMICS; DIAPAUSE; TEMPERATURE; TORTRICIDAE; PHENOLOGY; GRAPE; IMPACTS; TRANSMISSION AB We develop individual-based Monte Carlo methods to explore how climate change can alter insect voltinism under varying greenhouse gas emissions scenarios by using input distributions of diapause termination or spring emergence, development rate, and diapause initiation, linked to daily temperature and photoperiod. We show concurrence of these projections with a field dataset, and then explore changes in grape berry moth, Paralobesia viteana (Clemens), voltinism that may occur with climate projections developed from the average of three climate models using two different future emissions scenarios from the International Panel of Climate Change (IPCC). Based on historical climate data from 1960 to 2008, and projected downscaled climate data until 2099 under both high (A1fi) and low (B1) greenhouse gas emission scenarios, we used concepts of P. viteana biology to estimate distributions of individuals entering successive generations per year. Under the low emissions scenario, we observed an earlier emergence from diapause and a shift in mean voltinism from 2.8 to 3.1 generations per year, with a fraction of the population achieving a fourth generation. Under the high emissions scenario, up to 3.6 mean generations per year were projected by the end of this century, with a very small fraction of the population achieving a fifth generation. Changes in voltinism in this and other species in response to climate change likely will cause significant economic and ecological impacts, and the methods presented here can be readily adapted to other species for which the input distributions are reasonably approximated. C1 [Chen, Shi; Fleischer, Shelby J.; Saunders, Michael C.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Tobin, Patrick C.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. RP Chen, S (reprint author), Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. EM sxc445@psu.edu FU Pennsylvania State University FX We thank Melanie Fitzpatrick of the Union for Concerned Scientists and K. Hayhoe from Texas Tech University who provided us with climate data from 1960 to 2099. Funding was provided through a University Graduate Student Fellowship from Pennsylvania State University. NR 50 TC 7 Z9 8 U1 4 U2 19 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 505 EP 515 DI 10.1603/EN10099 PG 11 WC Entomology SC Entomology GA 810FS UT WOS:000294111700003 PM 22251628 ER PT J AU Jiang, XF Luo, LZ Zhang, L Sappington, TW Hu, Y AF Jiang, Xingfu Luo, Lizhi Zhang, Lei Sappington, Thomas W. Hu, Yi TI Regulation of Migration in Mythimna separata (Walker) in China: A Review Integrating Environmental, Physiological, Hormonal, Genetic, and Molecular Factors SO ENVIRONMENTAL ENTOMOLOGY LA English DT Review DE Mythimna separata; migration; regulation ID MANDUCA-SEXTA ALLATOTROPIN; OOGENESIS-FLIGHT SYNDROME; AGROTIS-IPSILON LEPIDOPTERA; AFRICAN ARMYWORM MOTH; LONG-DURATION FLIGHT; PSEUDALETIA-UNIPUNCTA; ORIENTAL ARMYWORM; JUVENILE-HORMONE; SPODOPTERA-EXEMPTA; TRUE ARMYWORM AB Each year the Mythimna separate (Walker), undertakes a seasonal, long-distance, multigeneration roundtrip migration between southern and northern China. Despite its regularity, the decision to migrate is facultative, and is controlled by environmental, physiological, hormonal, genetic, and molecular factors. Migrants take off on days 1 or 2 after eclosion, although the preoviposition period lasts approximate to 7 d. The trade-offs among the competing physiological demands of migration and reproduction are coordinated in M. separata by the "oogenesis-flight syndrome." Larvae that experience temperatures above or below certain thresholds accompanied by appropriate humidity, short photoperiod, poor nutrition, and moderate density tend to develop into migrants. However, there is a short window of sensitivity within 24 h after adult eclosion when migrants can be induced to switch to reproductive residents if they encounter extreme environmental factors including starvation, low temperature and long photoperiod. Juvenile hormone (JH) titer is low before migration but high titers are associated with termination of migratory behavior and the switch to reproduction. Early release of JH by the corpora allata in environmentally stressed 1-d old adults, otherwise destined by larval conditions to be migrants, switches them to residents. Offspring inherit parental additive genetic effects governing migratory behavior. However, they also retain flexibility in expression of both flight and reproductive life history traits. The insect neuropeptide, allatotropin, which activates corpora allata to synthesize JH, controls adult flight and reproduction. Future research directions to better understand regulation of migration in this species are discussed. C1 [Jiang, Xingfu; Luo, Lizhi; Zhang, Lei; Hu, Yi] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pest, Inst Plant Protect, Beijing 100193, Peoples R China. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Genet Lab, Ames, IA 50011 USA. RP Jiang, XF (reprint author), Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pest, Inst Plant Protect, 2 W Yuanmingyuan Rd Haidian Dist, Beijing 100193, Peoples R China. EM xfjiang@ippcaas.cn; lzluo@ippcaas.cn FU Major State Basic Research Development Program [2006CB102001]; National Natural Science Foundation of China [30871641, 31071641]; National Department of Public Benefit Research Foundation [200803007] FX We thank the USDA-ARS Corn Insects & Crop Genetics Research Unit for providing XFJ an opportunity to present his laboratory's cumulative research on this topic at the 2009 NCERA-148 meeting in Virginia, which generated valuable feedback and encouraged this review's composition. This work was funded by Projects under the Major State Basic Research Development Program (Grant 2006CB102001), National Natural Science Foundation of China (30871641, 31071641), and National Department of Public Benefit Research Foundation (200803007). NR 157 TC 13 Z9 21 U1 3 U2 43 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 516 EP 533 DI 10.1603/EN10199 PG 18 WC Entomology SC Entomology GA 810FS UT WOS:000294111700004 PM 22251629 ER PT J AU Zhang, MC Fielding, DJ AF Zhang, Mingchu Fielding, Dennis J. TI Populations of the Northern Grasshopper, Melanoplus borealis (Orthoptera: Acrididae), in Alaska Are Rarely Food Limited SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Orthoptera; Acrididae; resource limitation; bottom-up forces; subarctic ID MIXED-GRASS RANGELAND; INTERSPECIFIC COMPETITION; SANGUINIPES ORTHOPTERA; BEETLES COLEOPTERA; FORAGE DESTRUCTION; INSECT HERBIVORES; PERTURBATION; PREDATION; DYNAMICS; HABITAT AB In some systems, grasshoppers appear to be food limited in most years, whereas in other systems top down forces, for example, predators, are more often implicated in population regulation. Sustainable strategies to manage grasshopper populations through habitat management require knowledge of the forces that regulate grasshopper populations. This experiment was undertaken to determine whether populations of Melanoplus borealis (Fieber), a common pest species in Alaska, are food-limited in Alaska. Cages were set up in a fallow field near Delta Junction, AK, in 3 yr (2007-2009). In 2007 and 2008, fertilizer was added to half the plots to increase primary production, and, in all years, cages within each plot were stocked with 0, 5, 9, or 13 fourth-instar M. borealis ( equivalent to 0, 20, 36, or 52 grasshoppers/m(2)). Grasshoppers in each cage were counted weekly. Near the end of the growing season, surviving female grasshoppers (approximate to 40% of the original number) were collected. Femur length was taken as a measure of adult size, and functional ovarioles were counted as a measure of current fecundity. If the grasshoppers were food limited, we expected to see significant effects of either density or fertilizer on grasshopper survival, size, or fecundity. The fertilizer treatment greatly increased primary production in both years. Neither fertilizer treatment nor grasshopper density had consistent effects on survival, size, or potential fecundity, leading us to conclude that food is seldom limiting to populations in the interior of Alaska at densities <50 m(-2). C1 [Fielding, Dennis J.] USDA ARS, Subarctic Agr Res Unit, Fairbanks, AK 99775 USA. [Zhang, Mingchu] Univ Alaska, Sch Nat Resources & Agr Sci, Fairbanks, AK 99701 USA. RP Fielding, DJ (reprint author), USDA ARS, Subarctic Agr Res Unit, POB 757200, Fairbanks, AK 99775 USA. EM dennis.felding@ars.usda.gov NR 42 TC 2 Z9 2 U1 0 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 541 EP 548 DI 10.1603/EN10179 PG 8 WC Entomology SC Entomology GA 810FS UT WOS:000294111700006 ER PT J AU Oleiro, M Mc Kay, F Wheeler, GS AF Oleiro, Marina Mc Kay, Fernando Wheeler, Gregory S. TI Biology and Host Range of Tecmessa elegans (Lepidoptera: Notodontidae), a Leaf-Feeding Moth Evaluated as a Potential Biological Control Agent for Schinus terebinthifolius (Sapindales: Anacardiaceae) in the United States SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Brazilian pepper; weed biological control; exocrine gland; host specificity testing ID BRAZILIAN PEPPER; FLORIDA; CLASSIFICATION; CATERPILLARS; SPECIFICITY; SECRETION; CANDIDATE; DEFENSE; PLANTS; RADDI AB During surveys for natural enemies that could be used as classical biological control agents of Schinus terebinthifolius Raddi (Brazilian pepper), the caterpillar, Tecmessa elegans Schaus (Lepidoptera: Notodontidae), was recorded feeding on the leaves of the shrub in South America. The biology and larval and adult host range of this species were examined to determine the insect's suitability for biological control of this invasive weed in North America and Hawaii. Biological observations indicate that the larvae have five instars. When disturbed, the late instar larvae emit formic acid from a prothoracic gland that may protect larvae from generalist predators. Larval host range tests conducted both in South and North America indicated that this species feeds and completes development primarily on members of the Anacardiaceae within the tribe Rhoeae. Oviposition tests indicated that when given a choice in large cages the adults will select the target weed over Pistacia spp. However, considering the many valued plant species in its host range, especially several North American natives, this species will not be considered further for biological control of S. terebinthifolius in North America. C1 [Wheeler, Gregory S.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Oleiro, Marina; Mc Kay, Fernando] USDA ARS, S Amer Biol Control Lab, RA-1686 Hurlingham, Argentina. RP Wheeler, GS (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM greg.wheeler@ars.usda.gov FU Florida Fish and Wildlife Conservation Commission; South Florida Water Management District FX We thank K. Dyer and K. Paul, USDA-ARS-IPRL, J. Rendon, and M. Chawner SCA/AmeriCorps for technical assistance during quarantine host testing. Insect identifications were provided by M. Pogue, USDA-ARS-SEL. Voucher specimens have been placed at USDA-ARS-SEL and USDA-ARS-IPRL. We also thank A. McConnachie (ARC-PPRI), T. Heard (Commonwealth Scientific and Industrial Research Organization), A. Peard Bugliani, and J. Briano (SABCL) for their valuable comments and suggestions on the manuscript. Brazilian insect collections were conducted with the assistance of M. Vitorino, Universidade Regional de Blumenau, under the Instituto Brasileiro do Meio Ambiente permit 09BR003939/DF. Insects were imported under quarantine USDA/APHIS/PPQ permit P526P-07-06609 issued to G.S.W. This project was partially funded by Florida Fish and Wildlife Conservation Commission and South Florida Water Management District. NR 53 TC 11 Z9 11 U1 0 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 605 EP 613 DI 10.1603/EN10254 PG 9 WC Entomology SC Entomology GA 810FS UT WOS:000294111700013 PM 22251638 ER PT J AU Cane, JH Sampson, BJ Miller, SA AF Cane, James H. Sampson, Blair J. Miller, Stephanie A. TI Pollination Value of Male Bees: The Specialist Bee Peponapis pruinosa (Apidae) at Summer Squash (Cucurbita pepo) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE pollination efficiency; oligolecty; fruit set; fruit size; Cucurbitaceae ID FOETIDISSIMA CUCURBITACEAE; UNITED-STATES; SEX-RATIOS; HYMENOPTERA; APOIDEA; ANTHOPHORIDAE; ABUNDANCE; VACCINIUM; SIZE; XENOGLOSSA AB Male bees can be abundant at flowers, particularly floral hosts of those bee species whose females are taxonomic pollen specialists (oligolecty). Contributions of male bees to host pollination are rarely studied directly despite their prevalence in a number of pollination guilds, including those of some crop plants. In this study, males of the oligolectic bee, Peponapis pruinosa Say, were shown to be effective pollinators of summer squash, Cucurbita pepo L. Seven sequential visits from male P. pruinosa maximized squash fruit set and growth. This number of male visits accumulated during the first hour of their foraging and mate searching at flowers soon after sunrise. Pollination efficacy of male P. pruinosa and their abundances at squash flowers were sufficient to account for most summer squash production at our study sites, and by extrapolation, to two-thirds of all 87 North American farms and market gardens growing squashes that were surveyed for pollinators by collaborators in the Squash Pollinators of the Americas Survey. We posit that the substantial pollination value of male Peponapis bees is a consequence of their species' oligolecty, their mate seeking strategy, and some extreme traits of Cucurbita flowers (massive rewards, flower size, phenology). C1 [Cane, James H.; Miller, Stephanie A.] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Sampson, Blair J.] USDA ARS, Thad Cochran So Hort Lab, Poplarville, MS 39470 USA. RP Cane, JH (reprint author), Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. EM jim.cane@ars.usda.gov NR 37 TC 13 Z9 13 U1 7 U2 45 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 614 EP 620 DI 10.1603/EN10084 PG 7 WC Entomology SC Entomology GA 810FS UT WOS:000294111700014 PM 22251639 ER PT J AU Burks, CS Higbee, BS Siegel, JP Brandl, DG AF Burks, Charles S. Higbee, Bradley S. Siegel, Joel P. Brandl, David G. TI Comparison of Trapping for Eggs, Females, and Males of the Naval Orangeworm (Lepidoptera: Pyralidae) in Almonds SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE monitoring; egg trap; almonds; Prunis dulcis; Amyelois transitella ID NAVEL ORANGEWORM; SEXUAL COMMUNICATION; AMYELOIS-TRANSITELLA; MATING DISRUPTION; TRAPS; PHEROMONE; MOTH; PISTACHIOS; ABUNDANCE; ORCHARDS AB The navel orangeworm is the primary insect pest of almonds in California, and egg traps are the primary means of monitoring this pest. Aprevious study found that the current use of 2-4 traps per 64 ha block usually is not sufficient to provide management information specifically for that block. In this study, we compare data from large grids of egg traps in varied commercial almond orchards with trapping data for females and males, with the objective of finding a more cost-effective monitoring program using currently available attractants. The proportion of egg traps with eggs was highly correlated with mean eggs per egg trap, and with females and males trapped simultaneously at the same location. Almond variety and the type of bait used had little impact on the relationship between the proportion of egg traps with eggs and the number of eggs per traps. Traps in orchards with more unharvested (mummy) almonds had more eggs, suggesting that navel orangeworm abundance affected traps more than competition from mummies. Laboratory experiments comparing age-specific oviposition in two-choice and no-choice situations found that younger, more fecund females laid a greater proportion of eggs on the preferred substrate in a two-choice situation, but that age-specific fecundity was not different between substrates in no-choice tests. These findings indicate that the proportion of egg traps with eggs provides a more stable indication of navel orangeworm phenology than mean eggs per trap. We suggest that similar information could be obtained in a more cost-effective manner with female trapping. C1 [Burks, Charles S.; Siegel, Joel P.; Brandl, David G.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. RP Burks, CS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM charles.burks@ars.usda.gov OI Burks, Charles/0000-0003-0242-8644 NR 34 TC 11 Z9 11 U1 1 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 706 EP 713 DI 10.1603/EN10253 PG 8 WC Entomology SC Entomology GA 810FS UT WOS:000294111700025 PM 22251650 ER PT J AU Stuhl, C Sivinski, J Teal, P Paranhos, B Aluja, M AF Stuhl, Charles Sivinski, John Teal, Peter Paranhos, Beatriz Aluja, Martin TI A Compound Produced by Fruigivorous Tephritidae (Diptera) Larvae Promotes Oviposition Behavior by the Biological Control Agent Diachasmimorpha longicaudata (Hymenoptera: Braconidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Diachasmimorpha longicaudata; Bactrocera; Anastrepha; Ceratitis; para-ethylacetophenone ID CARIBBEAN FRUIT-FLY; HOST LOCATION; FLIES DIPTERA; STIMULI; PARASITOIDS; MORPHOLOGY; PHEROMONE; SUSPENSA; MEXICO; ORGANS AB Tephritid fruit fly parasitoids use fruit-derived chemical cues and the vibrations that result from larval movements to locate hosts sequestered inside fruit. However, compounds produced by the larvae themselves have not been previously described nor their significance to parasitoid foraging determined. We collected the volatiles from four species of tropical and subtropical Tephritidae: Anastrepha suspensa (Loew), Bactrocera dorsalis Hendel, Bactrocera cucurbitae Coquillett, and Ceratitis capitata (Wiedemann), representing two subfamilies (Dacinae and Trypetinae). Para-ethylacetophenone, an analog of a known tephritid parasitoid attractant, was a major constituent of all four, and was not associated with larvae of another acalypterate fly, Drosophila melanogaster Meigen, or with the calypterate Musca domestica L. It also was present in volatiles from whole, A. suspensa infested fruits of Eugenia uniflora (L.). Para-ethylacetophenone was not necessarily produced as a direct consequence of fruit consumption because it also was detected from larvae that developed in two artificial diets and in spent diets subsequent to larval development. Sensillae on both the antennae and ovipositor of the opiine braconid fruit fly parasitoid, Diachasmimorpha longicaudata (Ashmead) responded to the para-ethylacetophenone in larval volatiles and as a synthetic. Although a potential cue to foraging parasitoids, para-ethylacetophenone showed no long range (>1m) attractiveness to the adult female parasitoid, but did stimulate ovipositor-insertion and oviposition into both a natural (fruit) and an artificial (parafilm) substrate. Thus it may prove useful in colonizing and mass-rearing opine fruit fly parasitoids. C1 [Stuhl, Charles; Sivinski, John; Teal, Peter] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32604 USA. [Stuhl, Charles] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Paranhos, Beatriz] Embrapa Semi Arido, Entomol Lab, BR-56302970 Petrolina, PE, Brazil. [Aluja, Martin] Inst Ecol AC, Xalapa 91000, Veracruz, Mexico. RP Sivinski, J (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32604 USA. EM john.sivinski@ars.usda.gov FU CONACyT [79449] FX We thank Don McInnis, USDA-ARS (ret.), Honolulu, HI, for providing Bactrocera and Ceratitis larvae for volatile collections and the space to perform the collections in his laboratory. Tim Holler (USDA-APHIS [ret.]), allowed us to remove insects from his parasitoid colonies, helped with field collections, and was instrumental in performing many of the experiments. The Florida Division of Plant Industry generously supplied Anastrepha suspensa larvae. M. Aluja acknowledges support from CONACyT through a Sabbatical Year Fellowship (Ref. 79449) and thanks Benno Graf and Jorg Samietz (Forschungsanstalt Agroscope Changins-Wadenswil ACW, Switzerland) for providing ideal conditions to work on this paper. NR 39 TC 9 Z9 9 U1 2 U2 27 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2011 VL 40 IS 3 BP 727 EP 736 DI 10.1603/EN10198 PG 10 WC Entomology SC Entomology GA 810FS UT WOS:000294111700027 PM 22251652 ER PT J AU Schortemeyer, M Thomas, K Haack, RA Uzunovic, A Hoover, K Simpson, JA Grgurinovic, CA AF Schortemeyer, Marcus Thomas, Ken Haack, Robert A. Uzunovic, Adnan Hoover, Kelli Simpson, Jack A. Grgurinovic, Cheryl A. TI Appropriateness of Probit-9 in the Development of Quarantine Treatments for Timber and Timber Commodities SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE International Standard for Phytosanitary Measures No. 15; International Plant Protection Convention; quarantine treatments ID FRUIT-FLY DIPTERA; CODLING MOTH LEPIDOPTERA; HOT-WATER IMMERSION; EMERALD ASH BORER; ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; METHYL-BROMIDE FUMIGATION; VAPOR-HEAT-TREATMENT; ASIAN LONGHORNED BEETLE; LOW-TEMPERATURE STORAGE; MACONELLICOCCUS-HIRSUTUS HOMOPTERA AB Following the increasing international phasing out of methyl bromide for quarantine purposes, the development of alternative treatments for timber pests becomes imperative. The international accreditation of new quarantine treatments requires verification standards that give confidence in the effectiveness of a treatment. Probit-9 mortality is a standard for treatment effectiveness that has its origin in fruit fly research, and has been adopted by the United States Department of Agriculture for fruit flies and several other pests. Following this, the probit-9 standard has been adopted as a benchmark for many quarantine treatments worldwide. This article discusses aspects of the application of this concept for a range of timber pests. Problematic issues include the often small pest populations available for testing, the limits of modeling pest responses to a treatment in the absence of sufficient numbers for treatment verification, the species diversity of pests and host materials and the physical and chemical conditions of host material or treatment conditions. Where treatment verification by killing large numbers of individuals is impossible, data collected from small populations or under specific conditions must be interpreted with caution. We discuss possible alternative approaches to probit-9 as a treatment efficacy standard. C1 [Schortemeyer, Marcus; Thomas, Ken; Simpson, Jack A.; Grgurinovic, Cheryl A.] Dept Agr Fisheries & Forestry, Canberra, ACT 2601, Australia. [Schortemeyer, Marcus] Australian Natl Univ, Plant Sci Div, Res Sch Biol, Canberra, ACT 0200, Australia. [Haack, Robert A.] USDA Forest Serv, No Res Stn, E Lansing, MI 48823 USA. [Uzunovic, Adnan] FP Innovat, Vancouver, BC V6T 1W5, Canada. [Hoover, Kelli] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. RP Schortemeyer, M (reprint author), Dept Agr Fisheries & Forestry, GPO Box 858, Canberra, ACT 2601, Australia. NR 169 TC 8 Z9 8 U1 2 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 717 EP 731 DI 10.1603/EC10453 PG 15 WC Entomology SC Entomology GA 810IH UT WOS:000294118500002 PM 21735886 ER PT J AU Li, JL Peng, WJ Wu, J Strange, JP Boncristiani, H Chen, YP AF Li, Jilian Peng, Wenjun Wu, Jie Strange, James P. Boncristiani, Humberto Chen, Yanping TI Cross-Species Infection of Deformed Wing Virus Poses a New Threat to Pollinator Conservation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE honey bee; bumble bee; virus; host expansion ID HONEYBEES APIS-MELLIFERA; PCR ANALYSIS; BUMBLE BEES; DECLINE; VULNERABILITY; TRANSMISSION; PARASITES; PATHOGEN; PATTERNS; PLANTS AB The Deformed wing virus (family Iflaviridae, genus Iflavirus, DWV), one of the most prevalent and common viruses in honey bees, Apis mellifera L., is present in both laboratory-reared and wild populations of bumble bees, Bombus huntii Greene. Our studies showed thatDWVinfection spreads throughout the entire body ofB. huntii and that the concentration of DWV is higher in workers than in males both collected in the field and reared in the laboratory, implying a possible association between the virus infection and foraging activities. Further results showed that gut tissue of B. huntii can support the replication ofDWV, suggesting that B. huntii is a biological host for DWV, as are honey bees. Bumble bees and honey bees sometimes share nectar and pollen resources in the same field. The geographical proximity of two host species probably plays an important role in host range breadth of the virus. C1 [Boncristiani, Humberto; Chen, Yanping] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Li, Jilian; Peng, Wenjun; Wu, Jie] Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China. [Strange, James P.] Utah State Univ, USDA, ARS, Pollinating Insects Biol Management & Systemat La, Logan, UT 84322 USA. RP Chen, YP (reprint author), ARS, USDA, Bee Res Lab, BARC E Bldg 476, Beltsville, MD 20705 USA. EM judy.chen@ars.usda.gov FU Chinese National Nature Science Foundation [30700606, 30972149]; program for National Modern Apicultural Industry Technology Research System (Bee); USDA [2009-85118-05718] FX This work was funded by the Chinese National Nature Science Foundation, 30700606 and 30972149; the program for National Modern Apicultural Industry Technology Research System (Bee); and USDA-Coordinated Agricultural Project (CAP) grant 2009-85118-05718. NR 29 TC 21 Z9 26 U1 4 U2 47 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 732 EP 739 DI 10.1603/EC10355 PG 8 WC Entomology SC Entomology GA 810IH UT WOS:000294118500003 PM 21735887 ER PT J AU Danka, RG Harris, JW Villa, JD AF Danka, Robert G. Harris, Jeffrey W. Villa, Jose D. TI Expression of Varroa Sensitive Hygiene (VSH) in Commercial VSH Honey Bees (Hymenoptera: Apidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Apis mellifera; Varroa destructor; mite resistance; breeding ID DESTRUCTOR; RESISTANCE; MESOSTIGMATA; COLONIES AB We tested six commercial sources of honey bees, Apis mellifera L. (Hymenoptera: Apidae), whose breeding incorporated the trait of Varroa sensitive hygiene (VSH). VSH confers resistance to the parasitic mite Varroa destructor Anderson & Trueman by enhancing the ability of the bees to hygienically remove mite-infested brood. VSH production queens (i.e., queens commercially available for use in beekeepers' production colonies) from the six sources were established in colonies which later were measured for VSH. Their responses were compared with those of colonies with three other types of queens, as follows: VSH queens from the selected closed population maintained by USDA-ARS for research and as a source of breeding germplasm, queens from the cooperating commercial distributor of this germplasm, and queens of a commercial, mite-susceptible source. The reduction of mite infestation in brood combs exposed to test colonies for 1 wk differed significantly between groups. On average, colonies with VSH production queens reduced infestation by 44%. This group average was intermediate between the greater removal by pure ARS VSH (76%) and the cooperators' breeding colonies (64%), and the lesser removal by susceptible colonies (7%). VSH production colonies from the different sources had variable expression of hygiene against mites, with average reduced infestations ranging from 22 to 74%. In addition, infertility was high among mites that remained in infested cells in VSH breeder colonies from ARS and the commercial distributor but was lower and more variable in VSH production colonies and susceptible colonies. Commercial VSH production colonies supply mite resistance that generally seems to be useful for beekeeping. Resistance probably could be improved if more VSH drones sources were supplied when VSH production queens are being mated. C1 [Danka, Robert G.; Harris, Jeffrey W.; Villa, Jose D.] USDA ARS, Honey Bee Breeding Genet & Physiol Res Lab, Baton Rouge, LA 70820 USA. RP Danka, RG (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Res Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM bob.danka@ars.usda.gov NR 19 TC 4 Z9 5 U1 4 U2 20 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 745 EP 749 DI 10.1603/EC10401 PG 5 WC Entomology SC Entomology GA 810IH UT WOS:000294118500005 PM 21735889 ER PT J AU Stanley, CA Pitts-Singer, TL Bosch, J AF Stanley, Cory A. Pitts-Singer, Theresa L. Bosch, Jordi TI Influence of Rough Handling on Osmia lignaria (Hymenoptera: Megachilidae) Nest Establishment in Commercial Orchards SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Osmia lignaria; solitary bees; bee retention; bee dispersal ID SOLITARY BEE; BODY-SIZE; CORNUTA; SUCCESS; POLLINATOR; FEMALES AB Osmia lignaria Say (Hymenoptera: Megachilidae) can be used to pollinate fruit trees. Populations are sometimes difficult to sustain because some female bees fail to establish at provided nesting sites. We address the hypothesis that rough handling of overwintered O. lignaria results in decreased establishment. Wetested this by shaking (200 rpm for 2 min) overwintering bees as a proxy for rough handling. Bees were then released in an orchard, and nest establishment of shaken and unshaken bees was recorded. There was no significant difference in the proportion of shaken and unshaken females that nested, indicating that rough handling of overwintering bees does not discourage nest establishment. C1 [Stanley, Cory A.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Pitts-Singer, Theresa L.] USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Bosch, Jordi] Univ Autonoma Barcelona, CREAF, Bellaterra 08193, Spain. RP Stanley, CA (reprint author), Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. EM cory.vorel@usu.edu OI Bosch , Jordi/0000-0002-8088-9457 FU ARS USDA Bee Biology and Systematics Laboratory FX We thank Ron Zollinger for the use of his orchard. Appreciation is extended to employees of the ARS USDA Bee Biology and Systematics Laboratory, which assisted with and funded all work. NR 14 TC 1 Z9 1 U1 3 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 750 EP 752 DI 10.1603/EC10406 PG 3 WC Entomology SC Entomology GA 810IH UT WOS:000294118500006 PM 21735890 ER PT J AU Meikle, WG Patt, JM AF Meikle, William G. Patt, Joseph M. TI The Effects of Temperature, Diet, and Other Factors on Development, Survivorship, and Oviposition of Aethina tumida (Coleoptera: Nitidulidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aethina tumida; temperature; diet; survivorship; oviposition ID EUROPEAN HONEY-BEES; SIMULATION-MODEL; APIS-MELLIFERA; WEST-AFRICA; BEETLE; BIOLOGY; HETERORHABDITIDAE; STEINERNEMATIDAE; MANAGEMENT; MURRAY AB Developmental rate and survivorship of small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), life stages were measured across different temperatures (21, 25, 28, 32 and 35 degrees C) and diets, which included natural and artificial pollen, honey, and bee pupae. Temperature affected hatch success, time to hatching, and larval growth. Eggs hatched in 61 h at 21 degrees C but in <22 hat 35 degrees C. Larvae achieved peak weight in <8 d at 35 degrees C but needed 17 d at 21 degrees C. Diet had comparatively little effect on larval survivorship or maximum weight, although larvae fed only bee pupae had lower survivorship. Access to soil influenced pupation success. Duration of the life stage spent in the soil, during which pupation occurs, was also affected by temperature: adults emerged after 32.7 d at 21 degrees C but after only 14.8 d at 35 degrees C, albeit with high mortality. Minimum temperature for development was estimated at 13.5 degrees C for eggs, and 10.0 degrees C for larvae and pupae. Temperature influenced adult longevity and oviposition: on a honey and pollen diet average adult lifespan was 92.8 d at 24 degrees C but only 11.6 d at 35 degrees C. Beetles lived longer at 28 degrees C or lower but produced the most eggs per female, regardless of diet, at 32 degrees C. Beetle density influenced fecundity: beetles kept at three pairs per vial laid 6.7 times more eggs per female than those kept as single pairs. Overall, beetles fared best at 28-32 degrees C with mortality of all stages highest at 35 degrees C. C1 [Meikle, William G.; Patt, Joseph M.] USDA ARS, Kika Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Meikle, WG (reprint author), USDA ARS, Kika Garza Subtrop Agr Res Ctr, 2413 E Highway 83, Weslaco, TX 78596 USA. EM william.meikle@ars.usda.gov NR 25 TC 8 Z9 8 U1 0 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 753 EP 763 DI 10.1603/EC10364 PG 11 WC Entomology SC Entomology GA 810IH UT WOS:000294118500007 PM 21735891 ER PT J AU Prabhaker, N Castle, SJ Naranjo, SE Toscano, NC Morse, JG AF Prabhaker, Nilima Castle, Steven J. Naranjo, Steven E. Toscano, Nick C. Morse, Joseph G. TI Compatibility of Two Systemic Neonicotinoids, Imidacloprid and Thiamethoxam, With Various Natural Enemies of Agricultural Pests SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE systemic insecticides; beneficial insects; egg parasitoids; predators; laboratory bioassay ID HOMALODISCA-COAGULATA HOMOPTERA; INSECT GROWTH-REGULATORS; ORIUS-INSIDIOSUS; BEMISIA-TABACI; ARTHROPOD PREDATORS; WORKING GROUP; TOXICITY; CITRUS; PESTICIDES; CICADELLIDAE AB Two systemic neonicotinoids, imidacloprid and thiamethoxam, are widely used for residual control of several insect pests in cotton (Gossypium spp.), vegetables, and citrus (Citrus spp.). We evaluated their impact on six species of beneficial arthropods, including four parasitoid species-Aphytis melinus Debach, Gonatocerus ashmeadi Girault, Eretmocerus eremicus Rose & Zolnerowich, and Encarsia formosa Gahan-and two generalist predators-Geocoris punctipes (Say) and Orius insidiosus (Say)-in the laboratory by using a systemic uptake bioassay. Exposure to systemically treated leaves of both neonicotinoids had negative effects on adult survival in all four parasitoids, with higher potency against A. melinus as indicated by a low LC(50). Mortality was also high for G. ashmeadi, E. eremicus, and E. formosa after exposure to both compounds but only after 48 h posttreatment. The two predators G. punctipes and O. insidiosus were variably susceptible to imidacloprid and thiamethoxam after 96-h exposure. However, toxicity to these predators may be related to their feeding on foliage and not just contact with surface residues. Our laboratory results contradict suggestions of little impact of these systemic neonicotinoids on parasitoids or predators but field studies will be needed to better quantify the levels of such impacts under natural conditions. C1 [Prabhaker, Nilima; Toscano, Nick C.; Morse, Joseph G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Castle, Steven J.; Naranjo, Steven E.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. RP Prabhaker, N (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM nilima.castle@ucr.edu FU California Department of Food and Agriculture FX We thank Alan Urena and Paul Merten for technical assistance. Funding was provided in part by grants from the California Department of Food and Agriculture. NR 69 TC 28 Z9 37 U1 7 U2 48 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 773 EP 781 DI 10.1603/EC10362 PG 9 WC Entomology SC Entomology GA 810IH UT WOS:000294118500009 PM 21735893 ER PT J AU Yokoyama, VY AF Yokoyama, Victoria Y. TI Approved Quarantine Treatment for Hessian Fly (Diptera: Cecidomyiidae) in Large-Size Hay Bales and Hessian Fly and Cereal Leaf Beetle (Coleoptera: Chrysomelidae) Control by Bale Compression SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mayetiola destructor; Oulema melanopus; pressure; hydrogen phosphide; fumigation ID HYDROGEN PHOSPHIDE FUMIGATION; WRAPPED BALES; JAPAN; EFFICACY; EXPORT; DISINFESTATION; SIMULATION; MORTALITY; INSECTS; GRASSES AB A quarantine treatment using bale compression (32 kg/cm(2) pressure) and phosphine fumigation (61 g/28.3 m(3) aluminum phosphide for 7 d at 20 degrees C) was approved to control Hessian fly, Mayetiola destructor (Say), in large-size, polypropylene fabric-wrapped bales exported from the western states to Japan. No Hessian fly puparia (45,366) survived to the adult stage in infested wheat, Triticum aestivum L., seedlings exposed to the treatment in a large-scale commercial test. Daily temperatures (mean +/- SEM) inside and among bales in three test freight containers were 17.8 +/- 0.2 front top, 17.0 +/- 0.2 front bottom, 17.3 +/- 0.2 middle bale, 15.7 +/- 0.3 middle air, 18.5 +/- 0.1 back top, and 18.1 +/- 0.1 degrees C back bottom, allowing the fumigation temperature to be established at >= 20 degrees C. Mean fumigant concentrations ranged from 208 to 340 ppm during the first 3 d and ranged from 328 to 461 ppm after 7 d of fumigation. Copper plate corrosion values inside the doors, and in the middle of the large-size bales in all locations indicated moderate exposure to hydrogen phosphide (PH3). PH3 residues were below the U.S. Environmental Protection Agency tolerance of 0.1 ppm in animal feeds. The research was approved by Japan and U.S. regulatory agencies, and regulations were implemented on 20 May 2005. Compression in large-size bale compressors resulted in 3-3.6 and 0% survival of Hessian fly puparia and cereal leaf beetle, Oulema melanopus (L.), respectively. Bale compression can be used as a single treatment for cereal leaf beetle and as a component in a systems approach for quarantine control of Hessian fly. C1 USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Yokoyama, VY (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM victoria.yokoyama@ars.usda.gov FU National Hay Association; Kittitas County Timothy Hay Growers and Suppliers FX We especially thank Gina T. Miller and Gail E. Sergent (USDA-ARS, SJVASC) and Preston L. Hartsell (Dried Fruit Association, Fresno, CA) for participation in this project. We are grateful to Ron T. Anderson (The National Hay Association) for coordinating industry support and to Scott Isherwood, Denny Coates, Walter Hyde, Todd Fogelburg, and Chad Vessey (Anderson Hay and Grain, Ellensburg, WA); Richard Worth (Oregon Department of Agriculture, Salem, OR); and Steve VanMouwerik and Ron Toole (Anderson Hay and Grain, Aurora, OR) for field support. This research was supported in part by The National Hay Association and the Kittitas County Timothy Hay Growers and Suppliers. NR 26 TC 3 Z9 4 U1 2 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 792 EP 798 DI 10.1603/EC10339 PG 7 WC Entomology SC Entomology GA 810IH UT WOS:000294118500011 PM 21735895 ER PT J AU Blomefield, T Carpenter, JE Vreysen, MJB AF Blomefield, T. Carpenter, J. E. Vreysen, M. J. B. TI Quality of Mass-Reared Codling Moth (Lepidoptera: Tortricidae) After Long-Distance Transportation: 1. Logistics of Shipping Procedures and Quality Parameters as Measured in the Laboratory SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE sterile insect technique; codling moth; long-distance transportation ID CYDIA-POMONELLA LEPIDOPTERA; STERILE INSECT TECHNIQUE; SOUTH-AFRICA; OLETHREUTIDAE; POPULATIONS; COMPATIBILITY; TEMPERATURE; RADIATION; FERTILITY; FECUNDITY AB The sterile insect technique (SIT) is a proven effective control tactic against lepidopteran pests when applied in an areawide integrated pest management program. The construction of insect mass-rearing facilities requires considerable investment and moth control strategies that include the use of sterile insects could be made more cost-effective through the importation of sterile moths produced in other production centers. For codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), this is an attractive option because mating studies have confirmed the absence of mating barriers between codling moth populations from geographically different areas. To assess the feasibility of long-distance transportation of codling moths, pupae and adult moths were transported in 2004 from Canada to South Africa in four shipments by using normal commercial transport routes. The total transport time remained below 67 h in three of the consignments, but it was 89 h in the fourth consignment. Temperature in the shipping boxes was fairly constant and remained between -0.61 and 0.16 degrees C for 76.8-85.7% of the time. The data presented indicate that transporting codling moths as adults and pupae from Canada to South Africa had little effect on moth emergence, longevity, and ability to mate, as assessed in the laboratory. These results provide support to the suggestion that the SIT for codling moth in pome fruit production areas might be evaluated and implemented by the importation of irradiated moths from rearing facilities in a different country or hemisphere. C1 [Carpenter, J. E.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Blomefield, T.] Agr Res Council, ZA-7599 Stellenbosch, South Africa. [Vreysen, M. J. B.] IAEA, Insect Pest Control Lab, Joint FAO IAEA Programme Nucl Tech Food & Agr, A-1400 Vienna, Austria. RP Carpenter, JE (reprint author), USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. EM jcarpent@tifton.usda.gov FU Deciduous Fruit Producers Trust; ARC Infruitec-Nietvoorbij; International Atomic Energy Agency, Vienna FX We thank ARC Infruitec-Nietvoorbij for providing laboratory and cold room facilities and Muriel Knipe and Niel DuPlessis for invaluable technical assistance. Thanks to the Deciduous Fruit Producers Trust, ARC Infruitec-Nietvoorbij and the International Atomic Energy Agency, Vienna, for providing funds to conduct the research. We also appreciate the cooperation of the Okanagan-Kootenay Sterile Insect Release Programme in Osoyoos, BC, Canada, in conducting this research. NR 44 TC 9 Z9 12 U1 2 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 814 EP 822 DI 10.1603/EC10238 PG 9 WC Entomology SC Entomology GA 810IH UT WOS:000294118500014 PM 21735898 ER PT J AU Joseph, SV Hanula, JL Braman, SK Byrne, FJ AF Joseph, S. V. Hanula, J. L. Braman, S. K. Byrne, F. J. TI Effects of Fertilizer and Low Rates of Imidacloprid on Adelges tsugae (Hemiptera: Adelgidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE hemlock woolly adelgid; eastern hemlock; predators; imidacloprid; fertilizer ID LARICOBIUS-NIGRINUS COLEOPTERA; EASTERN UNITED-STATES; NITROGEN-FERTILIZATION; POPULATION-GROWTH; FOLIAR CHEMISTRY; MYZUS-PERSICAE; APHIS-GOSSYPII; NEW-ENGLAND; HOMOPTERA; DERODONTIDAE AB Healthy hemlock trees, Tsuga canadensis (L.) Carriere, and hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), populations should favor retention and population growth of adelgid predators such as Laricobius nigrinus Fender (Coleoptera: Derodontidae) and Sasajiscymnus tsugae (Sasaji & McClure) (Coleoptera: Coccinellidae). Eastern hemlock trees between 15 and 38 cm diameter at breast height (dbh) were treated with 0, 10, or 25% of 1.5 g imidacloprid (Merit 75 WP) per 2.5 cm dbh and were either fertilized or not, in a 3 by 2 factorial design. After 2 yr, imidacloprid reduced the numbers of ovisacs and eggs found on trees in a dosage-dependent manner, while enhancing tree growth parameters such as new shoots or needles and the length of new shoots. Fertilized trees had greater adelgid fecundity, which was positively correlated with total foliar N in both winter generations. In February 2009 (27 mo after imidacloprid treatment), higher imidacloprid dosages to unfertilized trees resulted in reduced adelgid fecundity. Concentrations of N, P, and K were higher in the foliage of trees treated with insecticide, whereas foliar aluminum concentrations were consistently lower in trees with higher insecticide dosages. Trees treated with low rates of imidacloprid were healthier than untreated trees, but only trees treated with the 0.1 x dosage had sufficient adelgids to possibly sustain predators over extended periods. C1 [Joseph, S. V.; Braman, S. K.] Univ Georgia, Dept Entomol, Griffin, GA 30223 USA. [Hanula, J. L.] USDA Forest Serv, Athens, GA 30602 USA. [Byrne, F. J.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Joseph, SV (reprint author), Univ Georgia, Dept Entomol, 1109 Expt St, Griffin, GA 30223 USA. EM shimat@uga.edu FU U.S. Forest Service, Southern Research Station [SRS 4552] FX We thank the Chattahoochee National Forest staff for help in getting the study established and S. Horn, M. Cody, C. Crowe, and J. Quick for technical assistance. We are grateful to C. Cheah for suggestions on assessing tree health and to J. Davis for providing valuable advice on statistical analysis. We thank D. Buntin, J. Ruberson, J. All, M. Ulyshen, and two anonymous reviewers for suggestions that greatly improved the manuscript. This project was funded by U.S. Forest Service, Southern Research Station SRS 4552. NR 50 TC 8 Z9 8 U1 0 U2 7 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 868 EP 878 DI 10.1603/EC10145 PG 11 WC Entomology SC Entomology GA 810IH UT WOS:000294118500022 PM 21735906 ER PT J AU Dodds, KJ AF Dodds, Kevin J. TI Effects of Habitat Type and Trap Placement on Captures of Bark (Coleoptera: Scolytidae) and Longhorned (Coleoptera: Cerambycidae) Beetles in Semiochemical-Baited Traps SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE disturbance; edge; survey; trapping; exotic species ID MULTIPLE-FUNNEL TRAPS; WOOD-BORING BEETLES; DOUGLAS-FIR BEETLE; DENDROCTONUS-PSEUDOTSUGAE; IPS-TYPOGRAPHUS; SAMPLING RANGE; FLIGHT; PHEROMONES; CATCHES; TREES AB Two studies were conducted to determine the effect of habitat selection and trap placement on catches of Scolytidae and Cerambycidae (Coleoptera) in northeastern U. S. forests. In a nonreplicated case study, four habitat types-closed canopy hardwood stand, closed canopy conifer stand, a low-intensity thinned Pinus strobus L. stand, and a high-intensity thinned P. strobus stand-were surveyed using alpha-pinene, ethanol, ipsenol, ipsdienol, and lanierone. Average trap catches, species richness, and the number of unique species captured were all highest in at least one of the thinned habitats. A second experiment that was replicated evaluated the placement of traps in relation to habitat patches. Semiochemical-baited traps (alpha-pinene and ethanol) were placed under a closed canopy forest, along an edge, and in a clearing and tested for effects on Scolytidae and Cerambycidae trap catches. Abundance and species richness were generally higher in the closed canopy and edge placements compared with traps in the open area. The highest number of unique species were captured in the edge and clearing. C1 US Forest Serv, NE Area State & Private Forestry, Durham Field Off, Durham, NH 03824 USA. RP Dodds, KJ (reprint author), US Forest Serv, NE Area State & Private Forestry, Durham Field Off, 271 Mast Rd, Durham, NH 03824 USA. EM kdodds@fs.fed.us NR 42 TC 10 Z9 10 U1 4 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 879 EP 888 DI 10.1603/EC10358 PG 10 WC Entomology SC Entomology GA 810IH UT WOS:000294118500023 PM 21735907 ER PT J AU Miller, DR Allison, JD AF Miller, Daniel R. Allison, Jeremy D. TI Variation in Enantiospecific Attraction of Ips avulsus (Coleoptera: Curculionidae) to the Pheromone Ipsdienol in Georgia SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE ipsdienol; enantiomers; Ips avulsus; semiochemical context; seasonal variation ID BARK BEETLES COLEOPTERA; SOUTHERN PINE-BEETLE; ENANTIOMERIC COMPOSITION; SEASONAL-VARIATION; SCOLYTIDAE; BEHAVIOR; COLONIZATION; AGGREGATION; LANIERONE AB In 2006, we tested the responses of the small southern pine engraver, Ips avulsus (Eichhoff) (Coleoptera: Curculionidae), to multiple-funnel traps baited with (+)-, (-)-, and (+/-)-ipsdienol. Three experiments were conducted in Georgia with all traps co-baited with one of the following lure combinations, respectively: experiment 1, ipsenol; experiment 2, lanierone and (-)- alpha-pinene; and experiment 3, ipsenol, lanierone, and (-)-alpha-pinene. Ipsdienol and lanierone are aggregation pheromones used by I. avulsus, whereas ipsenol is an aggregation pheromone used by the eastern fivespined ips, Ips grandicollis (Eichhoff), a sympatric species also breeding in pines. In all experiments, the highest catches of I. avulsus were obtained in traps baited with (+/-)-ipsdienol. The relative attractiveness of (+)-ipsdienol varied across the three experiments. When traps were co-baited with ipsenol, lanierone, and (-)-alpha-pinene, catches of I. avulsus in traps baited with (+)-ipsdienol were high and no different from those in traps baited with (+/-)-ipsdienol. When traps were co-baited with lanierone and (-)-alpha-pinene, catches in traps baited with (+)-ipsdienol were lower than those in traps baited with (+/-)-ipsdienol by approximate to 40%, whereas catches were reduced by approximate to 95% when traps were co-baited with ipsenol alone. We hypothesize that the observed variation in enantiospecific responses of I. avulsus to ipsdienol may be explained by variation in semiochemical context (i.e., different co-baits among the experiments) or seasonal variation. C1 [Miller, Daniel R.] USDA Forest Serv, So Res Stn, Athens, GA 30602 USA. [Allison, Jeremy D.] Louisiana State Univ, LSU AgCtr, Dept Entomol, Baton Rouge, LA 70803 USA. RP Miller, DR (reprint author), USDA Forest Serv, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM dmiller03@fs.fed.us FU USDA Forest Service; LSU AgCenter FX The use of trade names and identification of firms or corporations does not constitute an official endorsement or approval by the U.S. Government of any product or service to the exclusion of others that may be suitable. Funding for this research was provided by the USDA Forest Service and the LSU AgCenter. The USDA is an equal opportunity provider and employer. NR 41 TC 1 Z9 2 U1 0 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 895 EP 900 DI 10.1603/EC10405 PG 6 WC Entomology SC Entomology GA 810IH UT WOS:000294118500025 PM 21735909 ER PT J AU Francese, JA Fraser, I Lance, DR Mastro, VC AF Francese, Joseph A. Fraser, Ivich Lance, David R. Mastro, Victor C. TI Efficacy of Multifunnel Traps for Capturing Emerald Ash Borer (Coleoptera: Buprestidae): Effect of Color, Glue, and Other Trap Coatings SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE emerald ash borer; funnel trap; prism trap; visual cues; trap color ID AGRILUS-PLANIPENNIS COLEOPTERA; CERAMBYCIDAE; PLACEMENT; BEETLES; LURE AB Tens of thousands of adhesive-coated purple prism traps are deployed annually in the United States to survey for the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae). A reusable, more user-friendly trap is desired by program managers, surveyors, and researchers. Field assays were conducted in southeastern Michigan to ascertain the feasibility of using nonsticky traps as survey and detection tools for emerald ash borer. Three nonsticky trap designs, including multifunnel (Lindgren), modified intercept panel, and drainpipe (all painted purple) were compared with the standard purple prism trap; no statistical differences in capture of emerald ash borer adults were detected between the multifunnel design and the prism. In subsequent color comparison assays, both green-and purple-painted multifunnel traps (and later, plastic versions of these colors) performed as well or better than the prism traps. Multifunnel traps coated with spray-on adhesive caught more beetles than untreated traps. The increased catch, however, occurred in the traps' collection cups and not on the trap surface. In a separate assay, there was no significant difference detected between glue-coated traps and Rain-X (normally a glass treatment)-coated traps, but both caught significantly more A. planipennis adults than untreated traps. C1 [Francese, Joseph A.; Lance, David R.; Mastro, Victor C.] USDA, APHIS, PPQ CPHST, Otis Lab, Otis Angb, MA 02542 USA. [Fraser, Ivich] USDA, APHIS, PPQ, Emerald Ash Borer Project, Brighton, MI 48116 USA. RP Francese, JA (reprint author), USDA, APHIS, PPQ CPHST, Otis Lab, Bldg 1398, Otis Angb, MA 02542 USA. EM joe.francese@aphis.usda.gov FU USDA FX We thank the staff of the USDA-APHIS-PPQ CPHST Emerald Ash Borer laboratory (Brighton, MI): Michael Rietz, Nicole Smith, Janna Daimler, Ariel Andrews, Bethany Boegler, Kevin Donovan, Tony Kern, Joe Rogers, Grace O'Connor, Kelly Schmiermund, Ben Sorensen, Carissa Vanderschaaf, and Joshua Warlick for fieldwork assistance. Damon Crook and Alan Sawyer (Otis Laboratory) provided planning and analytical advice. Pete Holske (Otis Laboratory) provided trap painting assistance. Damon Crook, Phil Lewis (Otis Laboratory), and Jason Oliver (Tennessee State University, McMinnville, TN) reviewed an earlier version of this manuscript. This research was funded by the USDA-APHIS-PPQ Emerald Ash Borer Program. NR 27 TC 13 Z9 14 U1 1 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 901 EP 908 DI 10.1603/EC11038 PG 8 WC Entomology SC Entomology GA 810IH UT WOS:000294118500026 PM 21735910 ER PT J AU Yee, WL AF Yee, Wee L. TI Evaluation of Yellow Rectangle Traps Coated With Hot Melt Pressure Sensitive Adhesive and Sticky Gel Against Rhagoletis indifferens (Diptera: Tephritidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE western cherry fruit fly; yellow color; trap design; sticky gel; hot melt pressure sensitive adhesive ID MAGGOT FLIES DIPTERA; CHERRY FRUIT-FLY; APPLE MAGGOT; POMONELLA DIPTERA; RED SPHERES; LURES; ATTRACTION; FOOD AB Sticky yellow rectangle traps have been used for many years to capture Rhagoletis (Diptera: Tephritidae) fruit flies. Traditional sticky yellow traps are coated with a sticky gel (SG) that can leave residues on the hands of users. An alternative to SG on traps are hot melt pressure sensitive adhesives (HMPSAs), which are less messy. The main objective here was to evaluate two rectangle traps of two yellow colors, the Alpha Scents Yellow Card coated with HMPSA (Alpha Scents, West Linn, OR), and the Pherocon AM trap coated with SG (Pherocon; Trece, Adair, OK), for capturing western cherry fruit fly, Rhagoletis indifferens Curran. Flies captured on both traps and held in the laboratory and field did not escape their surfaces. Flies caught on HMPSA were damaged when removed from traps without citrus solvent, whereas flies caught on SG could be removed intact without solvent. In field tests, Alpha Scents traps baited with an ammonium bicarbonate lure captured 1.4-2.2 times more R. indifferens than Pherocon traps baited with the same lure. Results of an experiment that eliminated differences in surface sticky material type, overall size, and surface sticky area between Alpha Scents and Pherocon traps suggested, although did not show conclusively, that more flies were caught on the Alpha Scents than Pherocon traps because of their different yellow color and/or lower fluorescence and not the HMPSA. Overall, the Alpha Scents trap is a viable alternative to the Pherocon trap for detecting R. indifferens. C1 ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov NR 40 TC 8 Z9 8 U1 1 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 909 EP 919 DI 10.1603/EC10327 PG 11 WC Entomology SC Entomology GA 810IH UT WOS:000294118500027 PM 21735911 ER PT J AU Tooman, LK Rose, CJ Carraher, C Suckling, DM Paquette, SR Ledezma, LA Gilligan, TM Epstein, M Barr, NB Newcomb, RD AF Tooman, Leah K. Rose, Caroline J. Carraher, Colm Suckling, D. Max Paquette, Sebastien Rioux Ledezma, Lisa A. Gilligan, Todd M. Epstein, Marc Barr, Norman B. Newcomb, Richard D. TI Patterns of Mitochondrial Haplotype Diversity in the Invasive Pest Epiphyas postvittana (Lepidoptera: Tortricidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Epiphyas postvittana; mitochondrial population genetics; haplotype; invasive species; origin ID BROWN APPLE MOTH; EVOLUTIONARY GENETICS; POPULATION-GENETICS; DNA; CALIFORNIA; TEPHRITIDAE; BOTTLENECK; DIPTERA; BIOLOGY AB The light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), is a horticultural pest of Australia and New Zealand that has more recently invaded Hawaii, Europe, and California. A 2,216-bp region of the mitochondrial genome containing the cytochrome oxidase I and II genes was sequenced from 752 individuals. Haplotype network analyses revealed a major split between a predominantly Western Australian clade and all other samples, suggestive of either a deep genetic divergence or a cryptic species. Nucleotide and haplotype diversity were highest in the country of origin, Australia, and in New Zealand populations, with evidence of haplotype sharing between New Zealand and Tasmania. Nucleotide and haplotype diversity were higher in California than within the British Isles or Hawaii. From the total of 96 haplotypes, seven were found in California, of which four were private. Within California, there have been at least two introductions; based on genetic diversity we were unable to assign a likely source for a single moth found and eradicated in Los Angeles in 2007; however, our data suggest it is unlikely that Hawaii and the British Isles are sources of the major E. postvittana population found throughout the rest of the state since 2006. C1 [Tooman, Leah K.; Rose, Caroline J.; Carraher, Colm; Suckling, D. Max; Newcomb, Richard D.] New Zealand Inst Plant & Food Res Ltd, Auckland 1142, New Zealand. [Tooman, Leah K.; Paquette, Sebastien Rioux; Newcomb, Richard D.] Massey Univ, Allan Wilson Ctr Mol Ecol & Evolut, Palmerston North, New Zealand. [Paquette, Sebastien Rioux] Victoria Univ Wellington, Sch Biol Sci, Wellington 6140, New Zealand. [Ledezma, Lisa A.; Barr, Norman B.] USDA, APHIS, Edinburg, TX 78541 USA. [Gilligan, Todd M.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Epstein, Marc] Calif Dept Food & Agr, Plant Pest Diagnost Branch, Sacramento, CA 95832 USA. [Newcomb, Richard D.] Univ Auckland, Sch Biol Sci, Auckland 1142, New Zealand. RP Newcomb, RD (reprint author), New Zealand Inst Plant & Food Res Ltd, Private Bag 92169, Auckland 1142, New Zealand. EM richard.newcomb@plantandfood.co.nz RI Newcomb, Richard/C-2067-2009; Suckling, David/F-7005-2010; OI Newcomb, Richard/0000-0002-7307-9755; Suckling, David/0000-0001-7216-9348; Rioux Paquette, Sebastien/0000-0002-7724-6813 FU USDA-APHIS; Allan Wilson Centre for Molecular Ecology and Evolution FX We thank all those mentioned in Supp Table 2 [online only] for collecting samples. This work would not have been possible without their contributions. We are especially grateful to Lucia Varela for donating samples from Victoria, Bobbie Hitchcock for identifications and supplying specimens of E. ashworthana and E. sobrina, Bill Woods and coworkers for extensive sampling efforts in Western Australia, and Christian Mille for trapping efforts in New Caledonia. We also thank Tony Corbett for help with graphics; Jeremy McRae for R scripting; Anne Gunson for editorial suggestions; and Evan Braswell, Erik Rikkerink, David Teulon, and Jim Walker for comments on the manuscript. The research was funded by the USDA-APHIS and the Allan Wilson Centre for Molecular Ecology and Evolution. NR 48 TC 10 Z9 10 U1 2 U2 19 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 920 EP 932 DI 10.1603/EC10342 PG 13 WC Entomology SC Entomology GA 810IH UT WOS:000294118500028 PM 21735912 ER PT J AU Sanders, WR Liburd, OE Mankin, RW Meyer, WL Stelinski, LL AF Sanders, W. R. Liburd, O. E. Mankin, R. W. Meyer, W. L. Stelinski, L. L. TI Applications and Mechanisms of Wax-Based Semiochemical Dispenser Technology for Disruption of Grape Root Borer Mating SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Vitacea polistiformis; mating disruption; competitive attraction; pheromone dispenser ID ORIENTAL FRUIT MOTH; SEX-PHEROMONE; INTEGRATED CONTROL; PEACH ORCHARDS; LEPIDOPTERA; SESIIDAE; TORTRICIDAE; STRATEGIES; DENSITIES; DAMAGE AB Grape root borer, Vitacea polistiformis (Harris) (Lepidoptera: Sesiidae), is an important pest of cultivated grapes (Vitis spp.) in the eastern United States from Michigan to Florida. There are few registered insecticides for control of this pest, and their efficacy is limited. Pheromone-based mating disruption is a potential alternative to insecticides for management of V. polistiformis. Wax-based Specialized Pheromone & Lure Application Technology (SPLAT) was tested as a mating disruption method. Deployment densities of 150 dispensers per ha dosed with 5 mg of V. polistiformis pheromone were sufficient to achieve 95% mating disruption during a 7-wk trapping period. The disruption mechanism was determined to be competitive attraction. The release rate of pheromone from these dispensers was quantified to be approximately linear, 77.4 mu g/g SPLAT/d. At this release rate, a minimum initial load of 5.4 mg of pheromone per dispenser would be needed to maintain disruption over a 9-10-wk V. polistiformis flight season, approximate to 19 August to 21 October in central Florida. It should be noted, however, that the main pheromone component alone, (E,Z)-2,13-octadecadienyl acetate(ODDA), was effective (presumably by a noncompetitive mechanism) at higher loads per area of crop. Due to the cost of synthesis of highly pure isomers of the V. polistiformis pheromone components, mating disruption of V. polistiformis may be more practical with higher doses of commercially produced Zeuzera pyrina L. blend [95% (E, Z)-2,13-ODDA:5% (E, Z)-3,13-octadecadien-1-ol] or with (E, Z)-2,13-ODDA alone than with the V. polistiformis blend at lower rates. C1 [Meyer, W. L.; Stelinski, L. L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. [Sanders, W. R.; Liburd, O. E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Stelinski, LL (reprint author), Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. EM stelinski@ufl.edu FU Florida Grape Growers Association; Sustainable Agriculture Research and Education grant [G000340] FX We thank the staff in the Small Fruit and Vegetable IPM laboratory in Gainesville and the Applied Chemical Ecology laboratory in Lake Alfred at the University of Florida for assistance in field and laboratory work. We also thank the grape growers that participated in the study by providing their vineyards for our experiments including Bob Paulish, John Sirvent, and Antonio Fiorelli. Partial funding was provided by the Florida Grape Growers Association and Sustainable Agriculture Research and Education grant G000340. NR 28 TC 1 Z9 1 U1 0 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 939 EP 946 DI 10.1603/EC10359 PG 8 WC Entomology SC Entomology GA 810IH UT WOS:000294118500030 PM 21735914 ER PT J AU Ni, XZ Sparks, AN Riley, DG Li, XC AF Ni, Xinzhi Sparks, Alton N., Jr. Riley, David G. Li, Xianchun TI Impact of Applying Edible Oils to Silk Channels on Ear Pests of Sweet Corn SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE corn earworm; sap beetles; common smut; edible oils; husk coverage ID LEPIDOPTERAN PESTS; EARWORM CONTROL; VEGETABLE-OILS; STORED MAIZE; MAYSIN; THURINGIENSIS; LARVAE AB The impact of applying edible oils to corn silks on ear-feeding insects in sweet corn, Zea mays L., production was evaluated in 2006 and 2007. Six edible oils used in this experiment were canola, corn, olive, peanut, sesame, and soybean. Water and two commercial insecticidal oils (Neemix neem oil and nC21 Sunspray Ultrafine, a horticultural mineral oil) were used as the controls for the experiment. Six parameters evaluated in this experiment were corn earworm [Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)] damage rating, the number of sap beetle [Carpophilus spp. (Coleoptera: Nitidulidae)] adults and larvae, the number of corn silk fly (or picture-winged fly) (Diptera: Ulidiidae) larvae, common smut [Ustilago maydis (D. C.) Corda] infection rate, and corn husk coverage. Among the two control treatments, neem oil reduced corn earworm damage at both pre- and postpollination applications in 2006, but not in 2007, whereas the mineral oil applied at postpollination treatments reduced corn earworm damage in both years. The mineral oil also reduced the number of sap beetle adults, whereas the neem oil applied at postpollination attracted the most sap beetle adults in 2007. Among the six edible oil treatments, the corn and sesame oils applied at postpollination reduced corn earworm damage only in 2007. The application of the peanut oil at postpollination attracted more sap beetle adults in 2006, and more sap beetle larvae in 2007. Olive and neem oils significantly reduced husk coverage compared with the water control in both years. The mineral oil application consistently increased smut infection rate in both 2006 and 2007. Ramifications of using oil treatments in ear pest management also are discussed. C1 [Ni, Xinzhi] Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Sparks, Alton N., Jr.; Riley, David G.] Univ Georgia, Dept Entomol, Tifton, GA 31793 USA. [Li, Xianchun] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. RP Ni, XZ (reprint author), Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. EM xinzhi.ni@ars.usda.gov NR 27 TC 1 Z9 1 U1 1 U2 3 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 956 EP 964 DI 10.1603/EC10356 PG 9 WC Entomology SC Entomology GA 810IH UT WOS:000294118500032 PM 21735916 ER PT J AU Osbrink, WLA Cornelius, ML Lax, AR AF Osbrink, Weste L. A. Cornelius, Mary L. Lax, Alan R. TI Areawide Field Study on Effect of Three Chitin Synthesis Inhibitor Baits on Populations of Coptotermes formosanus and Reticulitermes flavipes (Isoptera: Rhinotermitidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Formosan termite; eastern subterranean termite; diflubenzuron; chlorfluazuron; hexaflumuron ID SUBTERRANEAN TERMITES ISOPTERA; SHIRAKI ISOPTERA; HEXAFLUMURON BAIT; NITROGEN-FIXATION; NEW-ORLEANS; MANAGEMENT; CHLORFLUAZURON; LOUISIANA; COLONIES; TOXICANT AB Periodic sampling of 43 independent monitors, initially active with Formosan subterranean termite, Coptotermes formosanus Shiraki, or the eastern subterranean termite, Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae), was conducted to evaluate the effects of cellulose baits containing one of three chitin synthesis inhibitors (CSIs)-diflubenzuron, hexaflumuron, or chlorfluazuron-on termite populations. Diflubenzuron at 0.1% active ingredient (AI, wt:wt) had no noticeable effect on termite populations. Chlorfluazuron (0.25% [AI]) significantly reduced termite populations in approximate to 3 yr. Chlorfluazuron used after >2-yr diflubenzuron treatment significantly reduced termite populations within months. This suggests diflubenzuron exposure increased the termite's sensitivity to chlorfluazuron accelerating population collapse. Hexaflumuron (0.5% [AI]) also reduced termite populations in approximate to 2 yr. The process of removing most detectable termite populations from the approximate to 160,000-m(2) campus of the Southern Regional Research Center, New Orleans, LA, with CSIs baits required approximate to 3 yr. Adjustments in the specific bait formulations and application procedures might reduce time to suppression. Establishment of new independent termite populations provides a mechanism to minimize the effects of baits. Remedial control measures around and under structures should be considered when implementing an area wide management strategy. C1 [Osbrink, Weste L. A.; Cornelius, Mary L.; Lax, Alan R.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Osbrink, WLA (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM weste.osbrink@ars.usda.gov NR 48 TC 11 Z9 12 U1 1 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1009 EP 1017 DI 10.1603/EC10217 PG 9 WC Entomology SC Entomology GA 810IH UT WOS:000294118500039 PM 21735923 ER PT J AU Cornelius, ML Osbrink, WLA AF Cornelius, Mary L. Osbrink, Weste L. A. TI Effect of Seasonal Changes in Soil Temperature and Moisture on Wood Consumption and Foraging Activity of Formosan Subterranean Termite (Isoptera: Rhinotermitidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE subterranean termite; feeding rates; monitoring station; seasonal trends ID NORTHERN CALIFORNIA; FEEDING-BEHAVIOR; NEW-ORLEANS; LOUISIANA; PATTERNS; COLONIES; PARK AB The objective of this study was to determine how seasonal changes affect the foraging activity and wood consumption of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae), in New Orleans, LA. There was a significant correlation between wood consumption and air temperature, soil temperature, and soil moisture, but not precipitation or number of rainy days. In the first year of the study, wood consumption was the lowest in December, February, and March. Wood consumption in January was not significantly different from consumption during the rest of the year. There were no seasonal changes in the number of underground monitoring stations occupied by termites. In the second year of the study, wood consumption was lowest from January to March. There was a significant decrease in the number of monitoring stations occupied by termites during the winter. This study determined that C. formosanus will remain in monitoring stations and resume feeding during warmer periods of a mild winter if average soil temperatures remain above 15 degrees C. Only prolonged periods of cold weather, with average soil temperatures below 15 degrees C, caused a significant number of termites to abandon underground monitoring stations. Seasonal changes in foraging activity would probably only disrupt baiting programs during severe winters in New Orleans, LA. C1 [Cornelius, Mary L.; Osbrink, Weste L. A.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Cornelius, ML (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM mary.cornelius@ars.usda.gov NR 23 TC 4 Z9 5 U1 1 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1024 EP 1030 DI 10.1603/EC10332 PG 7 WC Entomology SC Entomology GA 810IH UT WOS:000294118500041 PM 21735925 ER PT J AU Oswald, KJ French, BW Nielson, C Bagley, M AF Oswald, Kenneth J. French, B. Wade Nielson, Chad Bagley, Mark TI Selection for Cry3Bb1 Resistance in a Genetically Diverse Population of Nondiapausing Western Corn Rootworm (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; Bt resistance; MON863; insect resistance management ID VIRGIFERA-VIRGIFERA COLEOPTERA; BACILLUS-THURINGIENSIS TOXIN; DIABROTICA-VIRGIFERA; INSECT RESISTANCE; FITNESS COSTS; BT CORN; HERITABILITY; SUSCEPTIBILITY; LEPIDOPTERA; MANAGEMENT AB Five short-diapause laboratory lines of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), were selected for resistance to MON863, a variety of corn genetically modified with the Bacillus thuringiensis Berliner (Bt) transgene that expresses the Cry3Bb1 delta-endotoxin. Three of the selected lines were developed by incremental increase in the duration of exposure to MON863 over 11 generations (moderate selected lines). Two selected lines were developed from a control group by constant exposure to MON863 for at least 14 d posthatch over seven generations (intense selected lines). At the end of the experiment, survivorship, as measured by adult emergence, was approximate to 4 times higher in each of the selected lines reared on MON863 compared with control lines. Estimates of realized heritabilities (h(2)) were 0.16 and 0.15 for the moderate and intense selected lines, respectively, and are consistent with h(2) estimates reported previously from a variety of pest insects. These lines provide data necessary for evaluating the potential for Bt resistance within diabroticite beetles and will be useful for developing improved insect resistance management strategies. C1 [Oswald, Kenneth J.; Bagley, Mark] US EPA, Off Res & Dev, Natl Exposure Res Lab, Ecol Exposure Res Div,Mol Ecol Res Branch, Cincinnati, OH 45268 USA. [French, B. Wade; Nielson, Chad] ARS, N Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. RP Oswald, KJ (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab, Ecol Exposure Res Div,Mol Ecol Res Branch, Cincinnati, OH 45268 USA. EM oswald.kenneth@epa.gov NR 39 TC 32 Z9 33 U1 0 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1038 EP 1044 DI 10.1603/EC10312 PG 7 WC Entomology SC Entomology GA 810IH UT WOS:000294118500043 PM 21735927 ER PT J AU Meihls, LN Higdon, ML Ellersieck, M Hibbard, BE AF Meihls, Lisa N. Higdon, Matthew L. Ellersieck, Mark Hibbard, Bruce E. TI Selection for Resistance to mCry3A-Expressing Transgenic Corn in Western Corn Rootworm SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; Bacillus thuringiensis; MIR604; toxicity assay ID VIRGIFERA-VIRGIFERA COLEOPTERA; GAS-EXCHANGE PARAMETERS; TO-PLANT MOVEMENT; EVENT DAS-59122-7; LARVAE COLEOPTERA; CRY1F RESISTANCE; CHRYSOMELIDAE; MAIZE; INJURY; SUSCEPTIBILITY AB To investigate the development of resistance to mCry3A, a laboratory colony of the western corn rootworm, Diabrotica virgifera virgifera LeConte, was established from field survivors of mCry3A-expressing (MIR604) corn, Zea mays L. Feral adults emerging from MIR604 (selected) and isoline (control) field plots were collected and returned to the laboratory. Progeny of each colony was reared one generation on isoline corn and then crossed reciprocally with a nondiapausing colony. The resulting nondiapausing progeny were then reared on greenhouse corn in accordance with the wild type parent's origin (on MIR604 or isoline corn). After four, seven, and 10 total generations of selection, the resistance ratio of the selected colony was 0.5, 4.3, and 15.4 in terms of lethal concentration (LC)(50) values in toxicity assays, with the latter two LC(50) values being significant. After seven generations of selection in total, selected and control colonies were screened on MIR604 and isoline corn under field conditions. There was a significant colony x corn pedigree interaction in terms of plant damage. There was no significant difference in damage between MIR604 and isoline corn, whereas this difference was significant for the control colony. After 14 generations of selection, a seedling bioassay was performed. Again, there was a significant colony x corn pedigree interaction, this time in terms of the number of larvae recovered. There was no significant difference in the number of larvae recovered from MIR604 and isoline corn for the selected colony, whereas this difference was significant for the control colony, although larval size was greater on isoline corn for both colonies. Resistance has developed in western corn rootworm laboratory colonies to all Bt proteins currently registered for corn rootworm management, which emphasizes the importance of adhering to resistance management plans for maintaining product efficacy. C1 [Higdon, Matthew L.; Hibbard, Bruce E.] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [Meihls, Lisa N.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ellersieck, Mark] Univ Missouri, Agr Expt Stn, Columbia, MO 65211 USA. RP Hibbard, BE (reprint author), Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM bruce.hibbard@ars.usda.gov FU Syngenta Biotechnology Inc. [58-3K95-6-1167]; University of Missouri Division of Plant Science; USDA-ARS FX We thank Rebecca Bukowsky, Julie Barry, and Anthony Zukoff (USDA-ARS, Columbia, MO) for technical assistance along with a number of undergraduate workers. We thank Syngenta Seeds for providing Bt and isoline seed as well as protein and diet for toxicity bioassays. Finally, we thank Ryan Kurtz, Andre Crespo, Von Kaster (all with Syngenta), Blair Siegfried (University of Nebraska-Lincoln), and two anonymous reviewers for constructive comments on a previous draft. Funding for this work was provided by Syngenta Biotechnology Inc. through agreement 58-3K95-6-1167 to USDA-ARS, by the University of Missouri Division of Plant Science, and by USDA-ARS. NR 48 TC 35 Z9 36 U1 0 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1045 EP 1054 DI 10.1603/EC10320 PG 10 WC Entomology SC Entomology GA 810IH UT WOS:000294118500044 PM 21735928 ER PT J AU Wu, Y Li, YL Ruiz-Arce, R McPheron, BA Wu, JJ Li, ZH AF Wu, Yi Li, Yunlong Ruiz-Arce, Raul McPheron, Bruce A. Wu, Jiajiao Li, Zhihong TI Microsatellite Markers Reveal Population Structure and Low Gene Flow Among Collections of Bactrocera cucurbitae (Diptera: Tephritidae) in Asia SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bactrocera cucurbitae; quarantine pest; gene flow; genetic structure; microsatellites ID MEDITERRANEAN FRUIT-FLY; ALLELE FREQUENCY DATA; CERATITIS-CAPITATA; MITOCHONDRIAL-DNA; OLIVE FLY; MELON FLY; INVASION; DIFFERENTIATION; EXPANSION; SEQUENCES AB The melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), is widespread agricultural pest, and it is known to have the potential to establish invasive populations in various tropical and subtropical areas. Despite the economic risk associated with a putative stable presence of this fly, the population genetics of this pest have remained relatively unexplored in Asia, the main area for distribution of this pest. The goals for this study were to employ nuclear markers to examine geographic collections for population genetic structure and quantify the extent of gene flow within these Southeast Asian and Chinese populations. To achieve these goals, we used 12 polymorphic microsatellite markers. A low level of genetic diversity was found among collections from China and higher levels were seen in Southeast Asia collections. Three genetically distinct groups, Southeast Asia, southwest China, and southeast China, were recovered by Bayesian model-based clustering methods, the phylogenetic reconstruction and the principal coordinate analysis. The Mantel test clearly shows geographical distance contributed in the genetic structuring of B. cucurbitae's populations. No recent bottlenecks for any of the populations examined. The results of clustering, migration analyses, and Mantel test, strongly suggest that the regional structure observed may be due to geographical factors such as mountains, rivers, and islands. We found a high rate of migration in some sites from the southwest China region (cluster 1) and the southeast China region (cluster 2), suggesting that China-Guangdong-Guangzhou (GZ) may be the center of melon fruit fly in the southeast China region. C1 [Wu, Yi; Li, Yunlong; Li, Zhihong] China Agr Univ, Coll Agron & Biotechnol, Dept Entomol, Beijing 100193, Peoples R China. [Ruiz-Arce, Raul] USDA, APHIS, PPQ, Ctr Plant Hlth Sci & Technol,Mission Lab, Edinburg, TX 78541 USA. [McPheron, Bruce A.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Wu, Jiajiao] Guangdong Entry Exit Inspect & Quarantine Bur, Guangzhou 510623, Guangdong, Peoples R China. RP Li, ZH (reprint author), China Agr Univ, Coll Agron & Biotechnol, Dept Entomol, Yuanmingyuan W Rd 2, Beijing 100193, Peoples R China. EM lizh@cau.edu.cn OI McPheron, Bruce/0000-0001-9623-5100 FU National Natural Science Foundation of China [30771432]; Ministry of Agriculture of the People's Republic of China [2009-Z41, 200903034] FX We thank Guangqin Liang, Fan Liang for the melon fruit fly collection and identification. We also thank Warunee Sirikajornjaru and Prof. Edhi Marton for providing us the samples from Thailand and Indonesia, respectively. We are grateful to Md. Sajedul Islam and Samuel Aaron Gum for aid with software analysis, and W. Evan Braswell for a helpful manuscript review. Financial support for this work was provided by the National Natural Science Foundation of China (30771432) and the Ministry of Agriculture of the People's Republic of China (2009-Z41 and 200903034). NR 49 TC 5 Z9 6 U1 2 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1065 EP 1074 DI 10.1603/EC10395 PG 10 WC Entomology SC Entomology GA 810IH UT WOS:000294118500046 PM 21735930 ER PT J AU Chaudhury, MF Skoda, SR Sagel, A AF Chaudhury, M. F. Skoda, S. R. Sagel, A. TI Solidifying Agent and Processing of Blood Used for the Larval Diet Affect Screwworm (Diptera: Calliphoridae) Life-History Parameters SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cochliomyia hominivorax; insect diet; defibrination; anticoagulants ID SIZE AB Spray-dried whole bovine blood and a sodium polyacrylate polymer gel as a bulking and solidifying agent are among the constituents of the current larval diet for mass rearing screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Locally available, inexpensive dietary materials could reduce rearing cost and address an uncertain commercial supply of spray-dried blood. We compared efficacy of diet prepared from fresh bovine blood after decoagulation with sodium citrate or ethylenediaminetetraacetic acid (EDTA) or after mechanical defibrination, with the diet containing spray-dried blood using either gel or cellulose fiber as the bulking and solidifying agent. Several life-history parameters were compared among insects reared on each of the blood and bulking agent diets combination. Diets containing citrated blood yielded the lightest larval and pupal weights and fewest pupae. EDTA-treated blood with the gel also caused reductions. EDTA-treated blood with fiber yielded screwworms that were heavier and more numerous than those from the diet with citrated blood but lighter than those from the control diet using spray-dried blood. A reduction in percentage of adults emerging from pupae occurred from diets with both bulking agents using citrated blood and the diet using EDTA mixed with the gel bulking agent. As a group, the cellulose-fiber diets performed better than the gel diets. Larval diet did not affect adult longevity, weight of the eggs deposited by the females that emerged or subsequent egg hatch. Parameter measurements of insects from both defibrinated blood diets were similar to those from the spray-dried blood diets, indicating that fresh, defibrinated bovine blood can successfully replace the dry blood in the screwworm rearing medium. C1 [Chaudhury, M. F.; Sagel, A.] USDA ARS, Screwworm Res Unit, US Embassy Panama, Unit 9100, Dpo, AA 34002 USA. [Skoda, S. R.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Chaudhury, MF (reprint author), USDA ARS, Screwworm Res Unit, US Embassy Panama, Unit 9100, Box 4200, Dpo, AA 34002 USA. EM muhammad.chaudhury@ars.usda.gov NR 19 TC 4 Z9 4 U1 0 U2 2 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1103 EP 1107 DI 10.1603/EC10375 PG 5 WC Entomology SC Entomology GA 810IH UT WOS:000294118500051 PM 21735935 ER PT J AU Pitzer, JB Kaufman, PE Hogsette, JA Geden, CJ Tenbroeck, SH AF Pitzer, Jimmy B. Kaufman, Phillip E. Hogsette, Jerome A. Geden, Christopher J. Tenbroeck, Saundra H. TI Seasonal Abundance of Stable Flies and Filth Fly Pupal Parasitoids (Hymenoptera: Pteromalidae) at Florida Equine Facilities SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Stomoxys calcitrans; Spalangia; Muscidifurax; population dynamics; Musca domestica ID NASONIA-VITRIPENNIS HYMENOPTERA; STOMOXYS-CALCITRANS DIPTERA; ILLINOIS CATTLE FEEDLOTS; CONFINED BEEF-CATTLE; RELATIVE ABUNDANCE; DAIRY-CATTLE; MUSCIDIFURAX-RAPTORELLUS; NORTHWESTERN FLORIDA; CALIFORNIA DAIRIES; HOUSEFLIES DIPTERA AB Beginning in November 2007 and continuing until December 2009, weekly stable fly, Stomoxys calcitrans (L.), surveillance was conducted at four equine facilities near Ocala, FL, by using alsynite sticky traps for adults and by searching immature developmental sites for pupae. Adult stable fly trap captures were highly variable throughout the year, ranging from 0 to 1,400 flies per trap per farm. The greatest adult stable fly activity was observed during the spring months of March and April, with weekly three-trap means of 121 and 136 flies per farm, respectively. The importance of cultural control measures was most apparent on the only farm with no reported insecticide use and the lowest stable fly trap captures, where an intense daily sanitation and composting program was conducted. A survey of on-site filth fly pupae revealed that 99.9% of all parasitoids recovered were Spalangia spp., consisting of Spalangia cameroni Perkins (56.5%), Spalangia nigroaenea Curtis (34.0%), Spalangia endius Walker (5.8%), and Spalangia nigra Latreille (3.7%). The implications of these findings are discussed. C1 [Pitzer, Jimmy B.; Kaufman, Phillip E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Hogsette, Jerome A.; Geden, Christopher J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Tenbroeck, Saundra H.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. RP Kaufman, PE (reprint author), Univ Florida, Dept Entomol & Nematol, 970 Nat Area Dr, Gainesville, FL 32611 USA. EM pkaufman@ufl.edu OI Kaufman, Phillip/0000-0001-6159-8358 FU University of Florida Agricultural Experiment Station [FLA-ENY-04598, FLA-ENY-04880] FX We thank M. Bentley, S. Nunez, T. Johnson, M. Segal, P. Obenauer, and L. Wood for assistance with this project. Thanks also are extended to those equine facility owners allowing our projects to be conducted on their properties. This research was supported by the University of Florida Agricultural Experiment Station Federal Formula Funds, projects FLA-ENY-04598 and FLA-ENY-04880 (USDA-Co-operative State Research, Education and Extension Service). NR 44 TC 9 Z9 10 U1 1 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2011 VL 104 IS 3 BP 1108 EP 1115 DI 10.1603/EC10227 PG 8 WC Entomology SC Entomology GA 810IH UT WOS:000294118500052 PM 21735936 ER PT J AU Mellon, JE Vaughn, KC AF Mellon, J. E. Vaughn, K. C. TI Immunohistochemical investigation of cotton carpel tissue exposed to xylanolytic hydrolases of Aspergillus flavus SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Aspergillus flavus; Xylanolytic hydrolases; Virulence factor; Gossypium hirsutum; Carpel tissue ID CELL-WALLS; POLYGALACTURONASE; PATHOGENESIS AB Cotton carpel tissue (35-45 days post-anthesis) that had been treated with a mixture of xylanolytic hydrolases derived from Aspergillus flavus was subjected to immunocytochemical analysis. Microscopic examination of treated tissues revealed severe degradation of the secondary wall structure. Control tissue cells revealed the presence of high concentrations of xylans/arabinoxylans throughout the cell wall, as well as significant concentrations of arabinogalactan proteins in secondary wall structure. Carpel cells treated with a mixture of A. flavus-produced xylanolytic hydrolases showed a much reduced presence of labeling by xylan-specific antibodies on the inner wall surface, suggesting a severe loss of these plant polysaccharides in the secondary wall structure. Carpel exposure to a purified 14 kD endoxylanase from A. flavus also resulted in a severe reduction of xylans from secondary wall structure, although penetration of the tissue was not as dramatic. Arabinogalactan proteins were not as severely affected by the xylanolytic hydrolases. Comparison of control tissue with hydrolase-treated tissue stained with toluidine blue revealed an apparent reduction in wall thickness, supporting the conclusion of secondary wall structure degradation. Interestingly, the pectins could only be detected in the samples treated with xylanolytic enzymes, indicating that the pectins were being masked by xylans. These results are consistent with the conclusion that the xylanolytic hydrolase complex of A. flavus is a critical factor for host cell wall maceration and may represent another important fungal virulence factor, in addition to pectolytic hydrolase activities. Published by Elsevier Ltd. C1 [Mellon, J. E.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. [Vaughn, K. C.] ARS, USDA, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Mellon, JE (reprint author), ARS, USDA, So Reg Res Ctr, POB 19687, New Orleans, LA 70179 USA. EM Jay.Mellon@ars.usda.gov NR 21 TC 2 Z9 2 U1 1 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD JUN PY 2011 VL 76 IS 1 BP 34 EP 38 DI 10.1016/j.pmpp.2011.06.003 PG 5 WC Plant Sciences SC Plant Sciences GA 810FT UT WOS:000294111800006 ER PT J AU Klosterman, SJ Anchieta, A Garcia-Pedrajas, MD Maruthachalam, K Hayes, RJ Subbarao, KV AF Klosterman, Steven J. Anchieta, Amy Garcia-Pedrajas, Maria D. Maruthachalam, Karunakaran Hayes, Ryan J. Subbarao, Krishna V. TI SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa) SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Lactuca sativa; Lettuce; Verticillium dahliae; Susceptible; Suppression subtractive hybridization; Expression; SSH; Cysteine protease ID PROGRAMMED CELL-DEATH; REAL-TIME PCR; GENE-EXPRESSION; LEAF SENESCENCE; CYSTEINE PROTEINASES; ARABIDOPSIS-THALIANA; MOLECULAR ASPECTS; DAHLIAE; TOMATO; RESISTANCE AB Suppression subtractive hybridization (SSH) was employed to identify lettuce (Lactuca sativa) genes that are differentially expressed in symptomatic leaves infected with Verticillium dahliae. Genes expressed only in symptomatic leaves included those with homology to pathogenesis-related (PR) protein genes PR-3, PR-5, and a putative cysteine protease (LsCP2). Quantitative real-time reverse transcription PCR (RT-qPCR) revealed that LsCP2 was expressed in senescent leaves, but at significantly higher levels in symptomatic leaves. LsCP2 shares homology with plant cysteine proteases that are expressed in senescing leaves and may therefore accelerate or contribute to the leaf symptoms observed in a lettuce-V. dahliae interaction. Published by Elsevier Ltd. C1 [Klosterman, Steven J.; Anchieta, Amy; Hayes, Ryan J.] ARS, USDA, Salinas, CA 93905 USA. [Garcia-Pedrajas, Maria D.] Univ Malaga, Inst Hortofruticultura Subtrop & Mediterranea La, Consejo Super Invest Cient IHSM UMA CSIC, E-29750 Malaga, Spain. [Maruthachalam, Karunakaran; Subbarao, Krishna V.] Univ Calif Davis, Salinas, CA USA. RP Klosterman, SJ (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM Steve.Klosterman@ars.usda.gov RI Garcia-Pedrajas, Maria /G-1729-2015; OI Garcia-Pedrajas, Maria /0000-0001-5520-1887; Subbarao, Krishna/0000-0002-2075-1835 NR 54 TC 9 Z9 10 U1 1 U2 15 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD JUN PY 2011 VL 76 IS 1 BP 48 EP 58 DI 10.1016/j.pmpp.2011.05.003 PG 11 WC Plant Sciences SC Plant Sciences GA 810FT UT WOS:000294111800008 ER PT J AU Fleisher, DH Timlin, DJ Yang, Y Reddy, VR AF Fleisher, David H. Timlin, Dennis J. Yang, Yang Reddy, V. R. TI Potato Stem Density Effects on Canopy Development and Production SO POTATO RESEARCH LA English DT Article DE Canopy architecture; Lateral branching; Modelling; Potato; Stem density ID TUBER SIZE DISTRIBUTION; INDIVIDUAL LEAVES; LEAF APPEARANCE; SEED TUBERS; GROWTH; YIELD; PLANT; NITROGEN; TEMPERATURE; POPULATION AB Controlled environment studies with potato frequently assume responses from single-stem potato plants can be extrapolated to the field where multiple-stemmed plants are common. Controlled environment studies were conducted to characterize differences in canopy growth, development, and dry matter production between single- and multiple-stemmed potted potatoes. Leaf area distribution was influenced by stem density, with main stem and apical lateral branch leaf area being 150% to 200% larger in triple-stemmed (3S) versus single- stemmed (1S) pots (P<0.01), while basal lateral branch leaf area was 50% less (P<0.01). Basal lateral branches were more developed in 1S pots with longer branch lengths, more leaf area, and higher orders of branching. In a second experiment, more leaves were initiated in higher-density pots; however, individual leaf areas were approximately 50% smaller than those in 1S pots. Total leaf area and total, vegetative, and tuber dry matter production were unaffected by stem density in either experiment on a per pot basis. Development and growth of lower basal lateral branches in the 1S potato canopy offset any initial advantage in leaf numbers in the multiple-stemmed plants. These results appeared to validate the assumption that, given a late maturing cultivar and adequate nutrition, responses from single- stemmed plants can be extended to multiple-stemmed plants when expressed on a production area basis. These findings have practical considerations for potato researchers who conduct growth chamber experiments and potato modelers. C1 [Fleisher, David H.; Timlin, Dennis J.; Reddy, V. R.] USDA ARS PSI, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Yang, Yang] Dow AgroSci LLC, Indianapolis, IN 46268 USA. RP Fleisher, DH (reprint author), USDA ARS PSI, Crop Syst & Global Change Lab, Bldg 1,Rm 342,Barc W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM david.fleisher@ars.usda.gov NR 47 TC 0 Z9 0 U1 0 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-3065 J9 POTATO RES JI Potato Res. PD JUN PY 2011 VL 54 IS 2 BP 137 EP 155 DI 10.1007/s11540-011-9185-2 PG 19 WC Agronomy SC Agriculture GA 808UC UT WOS:000294003400003 ER PT J AU Nakasone, KK AF Nakasone, Karen K. TI Boidinella gen. nov (Cantharellales, Basidiomycota) SO CRYPTOGAMIE MYCOLOGIE LA English DT Article DE Taxonomy; cyanophilous basidiospores; dendrohyphidia; Dendrothele; urniform basidia ID FUNGI AB The genus Boidinella is erected to accommodate Sistotremella cystidiolophora and Dendrothele globulispora. The genus is characterized by effuse, soft, farinaceous to membranous basidiomata, delicate dendrohyphidia, obclavate leptocystidia, urniform basidia with 4 sterigmata, and subglobose to ellipsoid basidiospores with hyaline, slightly thickened, smooth, cyanophilous walls. Boidinella is compared with Dendrothele, Hypochnicium, Sistotremella, and Leptocorticium. C1 US Forest Serv, Ctr Forest Mycol Res, No Res Stn, Madison, WI 53726 USA. RP Nakasone, KK (reprint author), US Forest Serv, Ctr Forest Mycol Res, No Res Stn, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM knakasone@fs.fed.us NR 20 TC 1 Z9 1 U1 0 U2 0 PU ADAC-CRYPTOGAMIE PI PARIS PA 12 RUE DE BUFFON, 75005 PARIS, FRANCE SN 0181-1584 J9 CRYPTOGAMIE MYCOL JI Cryptogam. Mycol. PD JUN PY 2011 VL 32 IS 2 BP 191 EP 197 PG 7 WC Mycology SC Mycology GA 802EL UT WOS:000293492000009 ER PT J AU Crosslin, JM Lin, H Munyaneza, JE AF Crosslin, J. M. Lin, H. Munyaneza, J. E. TI Detection of 'Candidatus Liberibacter solanacearum' in the Potato Psyllid, Bactericera cockerelli (Sulc), by Conventional and Real-time PCR SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID ZEBRA CHIP DISEASE; ASSOCIATION; LEAFHOPPERS; PHYTOPLASMA; EUROPE; PLANTS AB 'Candidatus Liberibacter solanacearum' is the putative causal agent of zebra chip disease of potato, Solanum tuberosum L., and is transmitted by the potato psyllid, Bactericera cockerelli (Sulc). Much work has been done on the association of Liberibacter with the potato psyllid and several laboratories currently use polymerase chain reaction (PCR) to test for the bacterium. We have been testing for the bacterium in individual and composite samples of B. cockerelli as part of an ongoing investigation of the incidence of 'Ca. L. solanacearum' in potato psyllids collected in various parts of the US. Here we report that the bacterium can be reliably detected by using conventional and quantitative real-time PCR of nucleic acid extracts prepared from composite samples of one psyllid from a Liberibacter-infected colony combined with either one, two, three, four, five, nine, 14, 19, or 29 psyllids from a Liberibacter-free psyllid colony. This ability makes it possible to more easily test large numbers of psyllids for the presence of 'Ca. Liberibacter solanacearum'. This, in turn, will aid efforts to predict the threat of developing serious zebra chip disease as a result of psyllid infestations in potato fields. C1 [Crosslin, J. M.] USDA ARS, Prosser, WA 99350 USA. [Lin, H.] USDA ARS, Parlier, CA 93648 USA. [Munyaneza, J. E.] USDA ARS, Wapato, WA 98951 USA. RP Crosslin, JM (reprint author), USDA ARS, 24106 N Bunn Rd, Prosser, WA 99350 USA. FU Specialty Crops Research Initiative [2009-51181-20176] FX Funding for this research was provided by Specialty Crops Research Initiative grant 2009-51181-20176. USDA is an equal opportunity provider and employer. NR 19 TC 45 Z9 46 U1 1 U2 12 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2011 VL 36 IS 2 BP 125 EP 135 PG 11 WC Entomology SC Entomology GA 796ML UT WOS:000293055300002 ER PT J AU Armstrong, JS Coleman, RJ Adamczyk, JJ AF Armstrong, J. Scott Coleman, Randy J. Adamczyk, John J. TI Baseline Susceptibility of Creontiades signatus to Cotton Insecticides, with Emphasis on Malathion SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID PLANT BUG HETEROPTERA; MISSISSIPPI RIVER DELTA; HEMIPTERA-MIRIDAE; RESISTANCE; POPULATIONS; BIOASSAY; ACEPHATE; TEXAS AB We report the susceptibility of a green plant bug, Creontiades signatus Distant, a pest of cotton, Gossypium hirsutum L., and other crops in South Texas, to malathion and other cotton insecticides using the adult vial test, and an oral "floral foam" assay for systemic insecticides, when active eradication of boll weevil, Anthonomus grandis grandis Boheman, has been in effect for six seasons in the Rio Grande Valley of Texas. Field-collected green plant bugs exhibited malathion tolerance ratios of 1.68 to 4.83 when compared to unexposed plant bugs reared in the laboratory for six generations. The associated 95% C.I.'s for LC(50) values did not overlap following six consecutive paired (field versus laboratory) assays from November 2006 to October 2009. The greater tolerance ratios and LC(50) values may be the result of an average of 18 to 20 applications of ultra-low volume (ULV) malathion applied in the process of boll weevil eradication in cotton fields. Field-collected green plant bugs also had larger LC(50) values when exposed to acephate using an adult vial test compared to the laboratory strain, but the 95% C.I.s did not overlap. All other organophosphate, pyrethroid, carbamate, neonicotinoid, and flonicomid insecticides were very toxic against field- and laboratory-reared green plant bugs. The floral foam method of diluting non-acetone soluble insecticide active ingredients into honey-water worked well as an assay technique with the exception that mortality should be observed at 72 instead of 48 hours. C1 [Armstrong, J. Scott; Coleman, Randy J.; Adamczyk, John J.] USDA ARS, Beneficial Insects Res Unit, Weslaco, TX 78596 USA. RP Armstrong, JS (reprint author), USDA ARS, Beneficial Insects Res Unit, 2413 E Highway 83, Weslaco, TX 78596 USA. EM scott.armstrong@ars.usda.gov NR 21 TC 1 Z9 1 U1 1 U2 7 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2011 VL 36 IS 2 BP 145 EP 153 PG 9 WC Entomology SC Entomology GA 796ML UT WOS:000293055300004 ER PT J AU Lopez, JD Latheef, MA Hoffmann, WC AF Lopez, Juan D., Jr. Latheef, Mohammed A. Hoffmann, W. Clint TI Effect of Abamectin on Feeding Response, Mortality, and Reproduction of Adult Bollworm (Lepidoptera: Noctuidae) SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID HELICOVERPA-ZEA LEPIDOPTERA; MOTH LEPIDOPTERA; AVERMECTINS AB Newly eclosed adult bollworm, Helicoverpa zea (Boddie), feeds on carbohydrate from plants and other exudates before dispersal and reproduction. The objective of this study was to determine whether or not this nocturnal behavior could be used to attract the insect to a feeding-based attracticide mixed with a toxicant. Commercially-formulated abamectin (Agri-mek (R) 0.15EC) was evaluated as a toxicant mixed with a 2.5 M sucrose solution against bollworm adults, relative to toxicity, mortality, gustatory and proboscis-extension response, and reproduction. Sublethal doses of abamectin at 0.05, 0.1, 0.25, and 0.5 mg litre(-1) fed to laboratory-reared females within 24 hours of emergence significantly reduced by 10-73% the percentage of eggs that hatched as compared to females fed sucrose only. Mating frequency was significantly inhibited by 2-70% at 0.25 mg litre(-1) or more. A detailed examination of the effect of abamectin on toxicity, mortality, gustatory and proboscis-extension response, and reproduction of laboratory-reared and feral bollworms showed a potential exists for the use of this insecticide in a feeding-based adult control system. The dramatic decrease in fecundity and inhibition of mating frequency show a premise for the use of abamectin as a toxicant for suppression of bollworm adults. C1 [Lopez, Juan D., Jr.; Latheef, Mohammed A.; Hoffmann, W. Clint] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. RP Lopez, JD (reprint author), USDA ARS, Areawide Pest Management Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. EM clint.hoffmann@ars.usda.gov NR 17 TC 0 Z9 0 U1 0 U2 0 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2011 VL 36 IS 2 BP 155 EP 166 PG 12 WC Entomology SC Entomology GA 796ML UT WOS:000293055300005 ER PT J AU Ortega-Morales, AI Huerta, H Strickman, D Ramos, FJS Flores, JL Chavez, EC AF Ortega-Morales, Aldo I. Huerta, Heron Strickman, Daniel Sanchez Ramos, Francisco J. Landeros Flores, Jeronimo Cerna Chavez, Ernesto TI Records of Mosquitoes in Mexico: Culex stigmatosome Dyar and Cx. thriambus Dyar (Diptera: Culicidae) with Taxonomic Notes for Both Species SO SOUTHWESTERN ENTOMOLOGIST LA Spanish DT Article ID WEST-NILE-VIRUS; VECTOR COMPETENCE C1 [Ortega-Morales, Aldo I.; Landeros Flores, Jeronimo; Cerna Chavez, Ernesto] Univ Autonoma Agr Antonio Narro, Dept Parasitol, Saltillo 25315, Coahuila, Mexico. [Huerta, Heron] Inst Diagnost & Referencia Epidemiol, Entomol Lab, Mexico City 11340, DF, Mexico. [Strickman, Daniel] ARS, USDA, Beltsville, MD 20705 USA. [Sanchez Ramos, Francisco J.] Univ Autonoma Agr Antonio Narro, Dept Parasitol, Unidad Laguna, Torreon 27084, Coahuila, Mexico. RP Ortega-Morales, AI (reprint author), Univ Autonoma Agr Antonio Narro, Dept Parasitol, Calzada Antonio Narro 1923, Saltillo 25315, Coahuila, Mexico. EM agrortega@hotmail.com NR 36 TC 1 Z9 1 U1 0 U2 3 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2011 VL 36 IS 2 BP 177 EP 196 PG 20 WC Entomology SC Entomology GA 796ML UT WOS:000293055300007 ER PT J AU Esquivel, JF AF Esquivel, J. F. TI Euschistus servus (Say) - a New Host Record for Mermithidae (Mermithida) SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID BUG HEMIPTERA-PENTATOMIDAE; IMPROVED VISUALIZATION; NEMATODES; GREEN; CARPOCAPSAE; LARVAE; INDIA C1 ARS, USDA, So Plains Agr Res Ctr, Areawide Pest Management Res Unit, College Stn, TX USA. RP Esquivel, JF (reprint author), ARS, USDA, So Plains Agr Res Ctr, Areawide Pest Management Res Unit, College Stn, TX USA. EM Jesus.Esquivel@ars.usda.gov NR 18 TC 2 Z9 2 U1 0 U2 0 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2011 VL 36 IS 2 BP 207 EP 211 PG 5 WC Entomology SC Entomology GA 796ML UT WOS:000293055300010 ER PT J AU Samson, RA Peterson, SW Frisvad, JC Varga, J AF Samson, R. A. Peterson, S. W. Frisvad, J. C. Varga, J. TI New species in Aspergillus section Terrei SO STUDIES IN MYCOLOGY LA English DT Article DE Ascomycetes; Aspergillus section Terrei; beta-tubulin; calmodulin; citreoviridin; Eurotiales; extrolites; ITS; polyphasic taxonomy ID PULMONARY ASPERGILLOSIS; PHYLOGENETIC ANALYSIS; METABOLIC PRODUCTS; NIVEUS LU-9575; A-D; FUNGUS; MYCOTOXINS; STRAIN; MICROORGANISMS; CHROMATOGRAPHY AB Section Terrei of Aspergillus was studied using a polyphasic approach including sequence analysis of parts of the beta-tubulin and calmodulin genes and the ITS region, macro- and micromorphological analyses and examination of extrolite profiles to describe three new species in this section. Based on phylogenetic analysis of calmodulin and beta-tubulin sequences seven lineages were observed among isolates that have previously been treated as A. terreus and its subspecies by Raper & Fennell (1965) and others. Aspergillus alabamensis, A. terreus var. floccosus, A. terreus var. africanus, A. terreus var. aureus, A. hortai and A. terreus NRRL 4017 all represent distinct lineages from the A. terreus clade. Among them, A. terreus var. floccosus, A. terreus NRRL 4017 and A. terreus var. aureus could also be distinguished from A. terreus by using ITS sequence data. New names are proposed for A. terreus var. floccosus, A. terreus var. africanus, A. terreus var. aureus, while Aspergillus hortai is recognised at species level. Aspergillus terreus NRRL 4017 is described as the new species A. pseudoterreus. Also included in section Terrei are some species formerly placed in sections Flavipedes and Versicolores. A. clade including the type isolate of A. niveus (CBS 115.27) constitutes a lineage closely related to A. carneus. Fennellia nivea, the hypothesized teleomorph is not related to this clade. Aspergillus allahabadii, A. niveus var. indicus, and two species originally placed in section Versicolores, A. ambiguus and A. microcysticus, also form well-defined lineages on all trees. Species in Aspergillus section Terrei are producers of a diverse array of secondary metabolites. However, many of the species in the section produce different combinations of the following metabolites: acetylaranotin, asperphenamate, aspochalamins, aspulvinones, asteltoxin, asterric acid, asterriquinones, aszonalenins, atrovenetins, butyrolactones, citreoisocoumarins, citreoviridins, citrinins, decaturins, fulvic acid, geodins, gregatins, mevinolins, serantrypinone, terreic acid (only the precursor 3,6-dihydroxytoluquinone found), terreins, terrequinones, terretonins and territrems. The cholesterol-lowering agent mevinolin was found in A. terreus and A. neoafricanus only. The hepatotoxic extrolite citrinin was found in eight species: A. alabamensis, A. allahabadii, A. carneus, A. floccosus, A. hortai, A. neoindicus, A. niveus and A. pseudoterreus. The neurotoxic extrolite citreoviridin was found in five species: A. neoafricanus, A. aureoterreus, A. pseudoterreus, A. terreus and A. neoniveus. Territrems, tremorgenic extrolites, were found in some strains of A. alabamensis and A. terreus. C1 [Samson, R. A.; Varga, J.] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. [Peterson, S. W.] USDA ARS, Natl Ctr Agr Utilizat Res, Microbial Genom & Bioproc Res Unit, Peoria, IL 61604 USA. [Frisvad, J. C.] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby, Denmark. [Varga, J.] Univ Szeged, Fac Sci & Informat, Dept Microbiol, H-6726 Szeged, Hungary. RP Samson, RA (reprint author), CBS KNAW Fungal Biodivers Ctr, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands. EM r.samson@cbs.knaw.nl NR 69 TC 27 Z9 29 U1 2 U2 17 PU CENTRAALBUREAU SCHIMMELCULTURE PI UTRECHT PA PO BOX 85167, 3508 AD UTRECHT, NETHERLANDS SN 0166-0616 EI 1872-9797 J9 STUD MYCOL JI Stud. Mycol. PD JUN PY 2011 IS 69 BP 39 EP 55 DI 10.3114/sim.2011.69.04 PG 17 WC Mycology SC Mycology GA 804TT UT WOS:000293677600004 PM 21892242 ER PT J AU Chao, WS Xu, B Foley, ME AF Chao, W. S. Xu, B. Foley, M. E. TI Developing an Agrobacterium-mediated Transformation System for Leafy Spurge (Euphorbia esula), a Model System for Weeds SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Chao, W. S.; Foley, M. E.] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Xu, B.] Virginia Tech, Dept Hort, Blacksburg, VA 24060 USA. EM wun.chao@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S24 EP S25 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700058 ER PT J AU Dong, N McMahan, C AF Dong, Niu McMahan, Colleen TI Using Ribosome Binding Sequences for Multiple-Gene Transformation SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Dong, Niu; McMahan, Colleen] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. EM niu.dong@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S57 EP S58 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700136 ER PT J AU Ellis, D Hernandez-Ellis, M Leslie, C Jenderek, M AF Ellis, Dave Hernandez-Ellis, Margarita Leslie, Chuck Jenderek, Maria TI Cryopreservation of Walnut (Juglans regia L.) Embryogenic Cultures SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Ellis, Dave; Hernandez-Ellis, Margarita; Jenderek, Maria] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO USA. [Leslie, Chuck] Univ Calif Davis, Dept Pomol, Davis, CA 95616 USA. EM maria.jenderek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S63 EP S63 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700149 ER PT J AU Folta, KM Slovin, JP Atari, MFM Smith, K Gonzalez, L Needleman, H AF Folta, Kevin M. Slovin, Janet P. Atari, M. Fadhli Mad Smith, Kaylie Gonzalez, Laura Needleman, Hannah TI Strawberry Transformation as a Means to Accelerate Functional and Translational Studies in the Rosaceae SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Folta, Kevin M.; Atari, M. Fadhli Mad; Smith, Kaylie; Gonzalez, Laura; Needleman, Hannah] Univ Florida, Hort Sci Dept, Gainesville, FL 32611 USA. [Folta, Kevin M.; Atari, M. Fadhli Mad; Smith, Kaylie; Gonzalez, Laura; Needleman, Hannah] Univ Florida, Grad Program Plant Mol & Cellular Biol, Gainesville, FL 32611 USA. [Slovin, Janet P.] USDA, Small Fruit Lab, Beltsville, MD 20705 USA. EM kfolta@ufl.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S21 EP S21 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700047 ER PT J AU Hand, CP Reed, BM AF Hand, Charles P. Reed, Barbara M. TI The Effect of Explant Node Position on the Amount and Type of Bacterial Contamination in Hazelnut Shoot Cultures SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Hand, Charles P.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM handc@onid.orst.edu; Barbara.Reed@ars.usda.gov NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S67 EP S67 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700160 ER PT J AU Lisko, KA Hubstenberger, JF Belefant-Miller, H Phillips, GC Yan, WG McClung, A Lorence, A AF Lisko, Katherine A. Hubstenberger, John F. Belefant-Miller, Helen Phillips, Gregory C. Yan, Wen Gui McClung, Anna Lorence, Argelia TI Screening Rice Cultivars for Elevated Vitamin C Content SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Lisko, Katherine A.; Hubstenberger, John F.; Phillips, Gregory C.; Lorence, Argelia] Arkansas State Univ, Arkansas Biosci Inst, Stuttgart, AR USA. [Belefant-Miller, Helen; Yan, Wen Gui; McClung, Anna] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Phillips, Gregory C.] Arkansas State Univ, Coll Agr & Technol, Stuttgart, AR USA. [Lorence, Argelia] Arkansas State Univ, Dept Chem & Phys, Stuttgart, AR USA. EM Katherine.lisko@smail.astate.edu; alorence@astate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S61 EP S61 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700145 ER PT J AU Palla, KJ Beasley, RR Pijut, PM AF Palla, Kaitlin J. Beasley, Rochelle R. Pijut, Paula M. TI In Vitro Propagation of Persimmon (Diospyros virginiana L.) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Palla, Kaitlin J.; Beasley, Rochelle R.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM kpalla@purdue.edu; ppijut@purdue.edu RI Beasley, Rochelle/M-1396-2015; Pijut, Paula/N-6789-2015 OI Beasley, Rochelle/0000-0001-7325-4085; NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S68 EP S68 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700163 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI Improving Mineral Nutrition of Micropropagated Red Raspberry SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Poothong, Sukalya] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM sukalya_n@hotmail.com; Barbara.Reed@ars.usda.gov NR 0 TC 1 Z9 1 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S69 EP S69 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700165 ER PT J AU Rathore, KS Sundaram, S Sunilkumar, G Campbell, LM Tollack, LK Puckhaber, L Stipanovic, RD Palle, SR Krishnan, S AF Rathore, Keerti S. Sundaram, S. Sunilkumar, G. Campbell, L. M. Tollack, L. K. Puckhaber, Lorraine Stipanovic, Robert D. Palle, S. R. Krishnan, S. TI RNAi-mediated Metabolic Engineering of Cottonseed for Improving Its Nutritional Value SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rathore, Keerti S.; Sundaram, S.; Sunilkumar, G.; Campbell, L. M.; Tollack, L. K.; Palle, S. R.; Krishnan, S.] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA. [Puckhaber, Lorraine; Stipanovic, Robert D.] ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. EM rathore@tamu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S37 EP S38 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700088 ER PT J AU Reed, BM Wada, S DeNoma, J Evens, TJ Niedz, RP AF Reed, Barbara M. Wada, Sugae DeNoma, Jeanine Evens, Terrence J. Niedz, Randall P. TI Physiological Disorders of Pear Shoot Cultures SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Reed, Barbara M.; DeNoma, Jeanine] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Evens, Terrence J.; Niedz, Randall P.] US Hort Res Lab, Hort & Breeding Unit, Ft Pierce, FL 34945 USA. EM Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S18 EP S19 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700041 ER PT J AU Rupp, JL Fellers, JP Trick, HN AF Rupp, Jessica L. Fellers, John P. Trick, Harold N. TI RNAi Mediated Viral Resistance in Transgenic Wheat SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica L.; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Fellers, John P.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66506 USA. EM jrupp@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S36 EP S37 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700086 ER PT J AU Stevens, M Pijut, PM AF Stevens, M. Pijut, Paula M. TI Hypocotyl Derived In Vitro Regeneration of Pumpkin Ash (Fraxinus profunda) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Stevens, M.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM steven31@purdue.edu; ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 1 Z9 1 U1 0 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2011 VL 47 SU 1 BP S65 EP S66 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 799IZ UT WOS:000293281700156 ER PT J AU Barker, FM Snodderly, DM Johnson, EJ Schalch, W Koepcke, W Gerss, J Neuringer, M AF Barker, Felix M., II Snodderly, D. Max Johnson, Elizabeth J. Schalch, Wolfgang Koepcke, Wolfgang Gerss, Joachim Neuringer, Martha TI Nutritional Manipulation of Primate Retinas, V: Effects of Lutein, Zeaxanthin, and n-3 Fatty Acids on Retinal Sensitivity to Blue-Light-Induced Damage SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Article ID MACULAR PIGMENT DENSITY; AGE-RELATED MACULOPATHY; DOCOSAHEXAENOIC ACID; OXIDATIVE STRESS; RHESUS-MONKEYS; VITAMIN-C; OPTICAL-DENSITY; PROCTOR LECTURE; EYE DISEASE; DEGENERATION AB PURPOSE. Blue-light photooxidative damage has been implicated in the etiology of age-related macular degeneration (AMD). The macular pigment xanthophylls lutein (L) and zeaxanthin (Z) and n-3 fatty acids may reduce this damage and lower the risk of AMD. This study investigated the effects of the lifelong absence of xanthophylls followed by L or Z supplementation, combined with the effects of n-3 fatty acid deficiency, on acute blue-light photochemical damage. METHODS. Subjects included eight rhesus monkeys with no lifelong intake of xanthophylls and no detectable macular pigment. Of these, four had low n-3 fatty acid intake and four had adequate intakes. Control subjects had typical L, Z, and n-3 fatty acid intake. Retinas received 150-mu m-diameter exposures of low-power 476-nm laser light at 0.5 mm (similar to 2 degrees) eccentricity, which is adjacent to the macular pigment peak, and parafoveally at 1.5 mm (similar to 6 degrees). Exposures of xanthophyll-free animals were repeated after supplementation with pure L or Z for 22 to 28 weeks. Ophthalmoscopically visible lesion areas were plotted as a function of exposure energy, with greater slopes of the regression lines indicating greater sensitivity to damage. RESULTS. In control animals, the fovea was less sensitive to blue-light-induced damage than the parafovea. Foveal protection was absent in xanthophyll-free animals but was evident after supplementation. In the parafovea, animals low in n-3 fatty acids showed greater sensitivity to damage than animals with adequate levels. CONCLUSIONS. After long-term xanthophyll deficiency, L or Z supplementation protected the fovea from blue light-induced damage, whereas adequate n-3 fatty acid levels reduced the damage in the parafovea. (Invest Ophthalmol Vis Sci. 2011;52: 3934-3942) DOI:10.1167/iovs.10-5898 C1 [Neuringer, Martha] Oregon Reg Primate Res Ctr, Beaverton, OR 97006 USA. [Neuringer, Martha] Oregon Hlth & Sci Univ, Casey Eye Inst, Beaverton, OR USA. [Barker, Felix M., II] Salus Univ, Penn Coll Optometry, Elkins Pk, PA USA. [Snodderly, D. Max] Schepens Eye Res Inst, Boston, MA USA. [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Schalch, Wolfgang] DSM Nutr Prod Ltd, Kaiseraugst, Switzerland. [Koepcke, Wolfgang; Gerss, Joachim] Univ Munster, Dept Med Informat & Bioinformat, Munster, Germany. RP Neuringer, M (reprint author), Oregon Reg Primate Res Ctr, 505 NW 185th Ave, Beaverton, OR 97006 USA. EM neuringe@ohsu.edu RI GerSS, Joachim/B-5702-2013 FU DSM Nutritional Products Ltd., Kaiseraugst, Switzerland; Institute of Diabetes, Digestive and Kidney Diseases [DK29930]; National Center for Research Resources [RR00163]; U.S. Department of Agriculture [581950-9-001]; National Eye Institute [P30 EY03790]; Foundation Fighting Blindness FX Supported by DSM Nutritional Products Ltd., Kaiseraugst, Switzerland; Grant DK29930 from the Institute of Diabetes, Digestive and Kidney Diseases; Grant RR00163 from the National Center for Research Resources; Grant 581950-9-001 from the U.S. Department of Agriculture; Grant P30 EY03790 from the National Eye Institute; and a grant from The Foundation Fighting Blindness. NR 71 TC 57 Z9 57 U1 0 U2 12 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2011 VL 52 IS 7 BP 3934 EP 3942 DI 10.1167/iovs.10-5898 PG 9 WC Ophthalmology SC Ophthalmology GA 800BP UT WOS:000293332500003 PM 21245404 ER PT J AU Zhou, HY Zhao, XF Johnson, EJ Lim, A Sun, ED Yu, J Zhang, YB Liu, XP Snellingen, T Shang, F Liu, NP AF Zhou, Haiying Zhao, Xianfeng Johnson, Elizabeth J. Lim, Apiradee Sun, Erdan Yu, Jie Zhang, Yinbo Liu, Xipu Snellingen, Torkel Shang, Fu Liu, Ningpu TI Serum Carotenoids and Risk of Age-Related Macular Degeneration in a Chinese Population Sample SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Article ID VITAMIN-A; ADIPOSE-TISSUE; DIETARY-INTAKE; BETA-CAROTENE; ADULT-POPULATION; PIGMENT DENSITY; LUNG-CANCER; BEIJING EYE; ZEAXANTHIN; LUTEIN AB PURPOSE. It has been hypothesized that the macular carotenoids protect against age-related macular degeneration (AMD). In this study, the association between serum concentrations of carotenoids and the presence of AMD was evaluated in a case-control sample of elderly Chinese subjects. METHODS. Two hundred sixty-three individuals aged between 50 and 88 years enrolled in the study. Subjects included 82 cases with exudative AMD, 92 cases with early AMD, and 89 control individuals. Serum carotenoids (lutein, zeaxanthin, lycopene, alpha- and beta-carotenes, and beta-cryptoxanthin) and retinol were measured with reversed-phase high-performance liquid chromatography (HPLC). RESULTS. Serum levels of carotenoids and retinol were significantly lower in the cases with exudative AMD than in the controls. Median levels of lutein and zeaxanthin were 0.538 and 0.101 mu M, respectively, in the control subjects, and 0.488 and 0.076 mu M, respectively, in cases with exudative AMD. After adjustment for age, sex, smoking status, and body mass index (BMI), a significant inverse association was observed for exudative AMD with serum zeaxanthin (relative risk ratio [RRR], 0.04; 95% confidence interval [CI], 0-0.35), lycopene (RRR, 0.22; 95% CI, 0.1-0.48), and alpha-carotene (RRR, 0.24; 95% CI, 0.12-0.51). Early AMD was inversely associated only with lycopene (RRR, 0.49; 95% CI, 0.28-0.86) but was positively associated with alpha-carotene (RRR, 2.22; 95% CI, 1.37-3.58). No significant associations were observed between serum lutein and cases with early or exudative AMD. CONCLUSIONS. The data suggest that higher levels of serum carotenoids, in particular zeaxanthin and lycopene, are associated with a lower likelihood of having exudative AMD. Serum levels of carotenoids were relatively higher in this Chinese cohort than in samples of other ethnicities in previous reports. ( Invest Ophthalmol Vis Sci. 2011;52:4338-4344) DOI:10.1167/iovs.10-6519 C1 [Zhou, Haiying; Sun, Erdan; Yu, Jie; Zhang, Yinbo; Liu, Ningpu] Capital Med Univ, Beijing Tongren Eye Ctr, Beijing Tongren Hosp, Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China. [Zhao, Xianfeng] Chinese Ctr Dis Control & Prevent, Natl Inst Nutr & Food Safety, Beijing, Peoples R China. [Johnson, Elizabeth J.; Shang, Fu] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Lim, Apiradee] Prince Songkla Univ, Fac Sci & Technol, Dept Math & Comp Sci, Muang Pattani, Thailand. [Liu, Xipu; Snellingen, Torkel] Sekwa Eye Hosp, Beijing, Peoples R China. RP Liu, NP (reprint author), Capital Med Univ, Beijing Tongren Eye Ctr, Beijing Tongren Hosp, Beijing Ophthalmol & Visual Sci Key Lab, 1 Dong Jiao Min Xiang, Beijing 100730, Peoples R China. EM nliu001@gmail.com FU National Basic Research Program of China (Program 973) [2007CB512201]; Beijing Municipal Health Bureau [2009208] FX Supported by the National Basic Research Program of China (Program 973) Grant 2007CB512201 and the Beijing Municipal Health Bureau Grant 2009208. NR 53 TC 10 Z9 13 U1 0 U2 5 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2011 VL 52 IS 7 BP 4338 EP 4344 DI 10.1167/iovs.10-6519 PG 7 WC Ophthalmology SC Ophthalmology GA 800BP UT WOS:000293332500054 PM 21508112 ER PT J AU Tandon, S Woolverton, AE Landes, MR AF Tandon, Sharad Woolverton, Andrea E. Landes, Maurice R. TI Analyzing Modern Food Retailing Expansion Drivers in Developing Countries SO AGRIBUSINESS LA English DT Article ID RESEARCH AGENDA; SUPERMARKETS; RISE; ASIA AB The recent rapid growth in modern food retailing can be either driven by price decreases associated with efficiency gains in the supply chain over time, or it can be driven by growing consumer demand for nonprice characteristics, such as added convenience and higher quality goods. However, the price decreases associated with efficiency gains are likely to have a much larger effect on food demand and welfare in developing countries. Despite the importance of the question, few studies have empirically analyzed which driver has been more important to the spread of modern food retailing. Using a newly constructed data set of 103 developing countries, we find suggestive evidence of the expansion being primarily driven by growth in demand for nonprice characteristics. Specifically, we find that variation in demographic characteristics, such as share of the population who would benefit most from the convenience offered by modern formats, are associated with large variations in supermarket growth over the past decade. On the other hand, growth in modern food retailing appears uncorrelated with variation in the ease of developing efficient supply chains. Thus, although having the possibility to deliver large welfare gains to developing countries, the expansion of modern supermarkets likely has had much more muted effects. [EconLit citations: O100; L100; Q130]. (C) 2011 Wiley Periodicals, Inc. C1 [Tandon, Sharad; Woolverton, Andrea E.; Landes, Maurice R.] Econ Res Serv, USDA, Washington, DC 20036 USA. RP Tandon, S (reprint author), Econ Res Serv, USDA, 1800 M St NW, Washington, DC 20036 USA. EM standon@ers.usda.gov; woolverton.ae@gmail.com; mlandes@ers.usda.gov NR 30 TC 2 Z9 2 U1 1 U2 14 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0742-4477 J9 AGRIBUSINESS JI Agribusiness PD SUM PY 2011 VL 27 IS 3 BP 327 EP 343 DI 10.1002/agr.20267 PG 17 WC Agricultural Economics & Policy; Economics; Food Science & Technology SC Agriculture; Business & Economics; Food Science & Technology GA 796WF UT WOS:000293083500005 ER PT J AU Rothrock, PE Reznicek, AA Bryson, CT AF Rothrock, Paul E. Reznicek, Anton A. Bryson, Charles T. TI Geographic Range and Morphological and Chromosomal Variability of Carex molestiformis (Cyperaceae) East of the Mississippi River SO CASTANEA LA English DT Article AB Carex molestiformis, described in 1997 as an endemic to the Ozark and Ouachita Mountain regions, is newly collected from Georgia, Mississippi, and Ohio. Herbarium records also have confirmed this species from North Carolina, Virginia, and West Virginia bringing the overall range of this species to 11 states. A morphological comparison of C. molestiformis from east of the Mississippi River with western populations did not reveal regional differences. Some eastern populations occupied ruderal habitat, namely drier grassy roadsides and hay meadows rather than the river bottom openings typical of western populations. New chromosome counts for this species from the eastern portion of its range confirmed existing reports of n = 37 and also found agmatoploidy with n = 35. C1 [Rothrock, Paul E.] Taylor Univ, Randall Environm Studies Ctr, Upland, IN 46989 USA. [Reznicek, Anton A.] Univ Michigan Herbarium, Ann Arbor, MI 48108 USA. [Bryson, Charles T.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Rothrock, PE (reprint author), Taylor Univ, Randall Environm Studies Ctr, 236 W Reade Ave, Upland, IN 46989 USA. EM plrothroc@tayloru.edu NR 5 TC 0 Z9 1 U1 0 U2 1 PU SOUTHERN APPALACHIAN BOTANICAL SOC, NEWBERRY COLL PI NEWBERRY PA DEPT BIOLOGY, C/O CHARLES N HORN, SECRETARY-TREASURER, 2100 COLLEGE ST, NEWBERRY, SC 29108 USA SN 0008-7475 J9 CASTANEA JI Castanea PD JUN PY 2011 VL 76 IS 2 BP 178 EP 182 PG 5 WC Plant Sciences SC Plant Sciences GA 800NB UT WOS:000293366500006 ER PT J AU Zia-Ul-Haq, M Ahmad, S Shad, MA Iqbal, S Qayum, M Ahmad, A Luthria, DL Amarowicz, R AF Zia-ul-Haq, M. Ahmad, S. Shad, M. Aslam Iqbal, S. Qayum, M. Ahmad, A. Luthria, D. L. Amarowicz, R. TI COMPOSITIONAL STUDIES OF LENTIL (LENS CULINARIS MEDIK.) CULTIVARS COMMONLY GROWN IN PAKISTAN SO PAKISTAN JOURNAL OF BOTANY LA English DT Article ID IMPATIENS-BICOLOR-ROYLE; CHICKPEA; LEGUMES; SEEDS; OIL; TOCOPHEROL; FRACTIONS; QUALITY AB Four improved lentil cultivars viz., Masoor 85, Masoor 93, NIAB Masoor 2002 and NIAB Masoor 2006 grown and consumed in Pakistan have been systematically analyzed to determine and compare their nutritional and compositional properties. Proximate analysis, anti-nutritional contents, amino acid and fatty acid profiles of the oil extracted along with mineral content from all four cultivars were investigated. Mineral composition showed that sufficient amounts of Ca, P, K, Cu, Zn and Mg were present to meet the macro and micro-nutrients demand in human diets. Despite variations, potassium and manganese were noted as being present in highest and lowest concentrations, respectively, in all cultivars. The distribution patterns of various amino acids in these cultivars suggested sulfur containing amino acids as limiting amino acids. Fatty acid profile indicated unsaturated fatty acids as major fatty acids in all cultivars. The data show that, in terms of both quality and quantity, all four lentil cultivars can serve as a significant source of essential amino acids, essential fatty acids and trace minerals to meet the demand of population of Pakistan. C1 [Ahmad, S.] Bahauddin Zakariya Univ, Dept Agron, Multan 60800, Pakistan. [Shad, M. Aslam] Bahauddin Zakariya Univ, Dept Chem, Multan 60800, Pakistan. [Zia-ul-Haq, M.] Univ Karachi, Dept Pharmacognosy, Karachi 75270, Pakistan. [Iqbal, S.] Univ Sargodha, Dept Chem, Sargodha 40100, Pakistan. [Qayum, M.] Univ Peshawar, Dept Pharm, Peshawar 25120, Pakistan. [Ahmad, A.] Univ Agr Faisalabad, Agroclimatol Lab, Dept Agron, Faisalabad 38040, Pakistan. [Luthria, D. L.] ARS, Food Composit & Method Dev Lab, USDA, BHNRC, Beltsville, MD 20705 USA. [Amarowicz, R.] Polish Acad Sci, Inst Anim & Reprod & Food Res, Dept Chem & Phys Properties Food, PL-10747 Olsztyn, Poland. RP Ahmad, S (reprint author), Bahauddin Zakariya Univ, Dept Agron, Multan 60800, Pakistan. EM shakeel.agronomy@gmail.com RI Zia-Ul-Haq, Muhamamd/D-7779-2011; Iqbal, Shahid/I-8030-2015; OI Ahmad, Shakeel/0000-0002-8613-2703; AMAROWICZ, Ryszard/0000-0001-9731-0045 NR 39 TC 23 Z9 24 U1 1 U2 9 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 J9 PAK J BOT JI Pak. J. Bot. PD JUN PY 2011 VL 43 IS 3 BP 1563 EP 1567 PG 5 WC Plant Sciences SC Plant Sciences GA 800DO UT WOS:000293340200024 ER PT J AU Myers, AM James, MG Lin, QH Yi, G Stinard, PS Hennen-Bierwagen, TA Becraft, PW AF Myers, Alan M. James, Martha G. Lin, Qiaohui Yi, Gibum Stinard, Philip S. Hennen-Bierwagen, Tracie A. Becraft, Philip W. TI Maize opaque5 Encodes Monogalactosyldiacylglycerol Synthase and Specifically Affects Galactolipids Necessary for Amyloplast and Chloroplast Function SO PLANT CELL LA English DT Article ID ASSISTED CARBOHYDRATE ELECTROPHORESIS; ZEA-MAYS-L; STARCH GRANULES; ARABIDOPSIS-THALIANA; ANGSTROM RESOLUTION; ENVELOPE MEMBRANES; RICE ENDOSPERM; INNER ENVELOPE; PHOTOSYSTEM-II; PROTEIN AB The maize (Zea mays) opaque5 (o5) locus was shown to encode the monogalactosyldiacylglycerol synthase MGD1. Null and point mutations of o5 that affect the vitreous nature of mature endosperm engendered an allelic series of lines with stepwise reductions in gene function. C-18:3/C-18:2 galactolipid abundance in seedling leaves was reduced proportionally, without significant effects on total galactolipid content. This alteration in polar lipid composition disrupted the organization of thylakoid membranes into granal stacks. Total galactolipid abundance in endosperm was strongly reduced in o5-mutants, causing developmental defects and changes in starch production such that the normal simple granules were replaced with compound granules separated by amyloplast membrane. Complete loss of MGD1 function in a null mutant caused kernel lethality owing to failure in both endosperm and embryo development. The data demonstrate that low-abundance galactolipids with five double bonds serve functions in plastid membranes that are not replaced by the predominant species with six double bonds. Furthermore, the data identify a function of amyloplast membranes in the development of starch granules. Finally, the specific changes in lipid composition suggest that MGD1 can distinguish the constituency of acyl groups on its diacylglycerol substrate based upon the degree of desaturation. C1 [Yi, Gibum; Becraft, Philip W.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Myers, Alan M.; James, Martha G.; Lin, Qiaohui; Hennen-Bierwagen, Tracie A.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Stinard, Philip S.] ARS, USDA, Maize Genet Cooperat Stock Ctr, Urbana, IL 61801 USA. RP Becraft, PW (reprint author), Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. EM becraft@iastate.edu OI Becraft, Philip/0000-0002-3299-2126; Yi, Gibum/0000-0002-7630-1892; Myers, Alan/0000-0002-9619-4692 NR 81 TC 26 Z9 28 U1 2 U2 12 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD JUN PY 2011 VL 23 IS 6 BP 2331 EP 2347 DI 10.1105/tpc.111.087205 PG 17 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 798PU UT WOS:000293224200024 PM 21685260 ER PT J AU Johnson, DM McCulloh, KA Meinzer, FC Woodruff, DR Eissenstat, DM AF Johnson, D. M. McCulloh, K. A. Meinzer, F. C. Woodruff, D. R. Eissenstat, D. M. TI Hydraulic patterns and safety margins, from stem to stomata, in three eastern US tree species SO TREE PHYSIOLOGY LA English DT Article DE cavitation; embolism; photosynthesis; transpiration; xylem ID AIR-SEEDING THRESHOLDS; LEAF HYDRAULICS; TROPICAL SAVANNA; XYLEM CAVITATION; LIGHT RESPONSE; GAS-EXCHANGE; PINE NEEDLES; DOUGLAS-FIR; SUGAR MAPLE; SAP FLOW AB Adequate water transport is necessary to prevent stomatal closure and allow for photosynthesis. Dysfunction in the water transport pathway can result in stomatal closure, and can be deleterious to overall plant health and survival. Although much is known about small branch hydraulics, little is known about the coordination of leaf and stem hydraulic function. Additionally, the daily variations in leaf hydraulic conductance (K(leaf)), stomatal conductance and water potential (psi(L)) have only been measured for a few species. The objective of the current study was to characterize stem and leaf vulnerability to hydraulic dysfunction for three eastern US tree species (Acer rubrum, Liriodendron tulipifera and Pinus virginiana) and to measure in situ daily patterns of K(leaf), leaf and stem psi, and stomatal conductance in the field. Sap flow measurements were made on two of the three species to compare patterns of whole-plant water use with changes in K(leaf) and stomatal conductance. Overall, stems were more resistant to hydraulic dysfunction than leaves. Stem P(50) (psi resulting in 50% loss in conductivity) ranged from -3.0 to -4.2 MPa, whereas leaf P50 ranged from -0.8 to -1.7 MPa. Field psi(L) declined over the course of the day, but only P. virginiana experienced reductions in K(leaf) (nearly 100% loss). Stomatal conductance was greatest overall in P. virginiana, but peaked midmorning and then declined in all three species. Midday stem. in all three species remained well above the threshold for embolism formation. The daily course of sap flux in P. virginiana was bell-shaped, whereas in A. rubrum sap flux peaked early in the morning and then declined over the remainder of the day. An analysis of our data and data for 39 other species suggest that there may be at least three distinct trajectories of relationships between maximum K(leaf) and the % K(leaf) at psi(min). In one group of species, a trade-off between maximum K(leaf) and % K(leaf) at psi(min) appeared to exist, but no trade-off was evident in the other two trajectories. C1 [Johnson, D. M.] Ohio Univ, Dept Environm & Plant Biol, Athens, OH 45701 USA. [McCulloh, K. A.] Oregon State Univ, Dept Wood Sci & Engn, Corvallis, OR 97331 USA. [Meinzer, F. C.; Woodruff, D. R.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Eissenstat, D. M.] Penn State Univ, Dept Hort, University Pk, PA 16802 USA. RP Johnson, DM (reprint author), Ohio Univ, Dept Environm & Plant Biol, Athens, OH 45701 USA. EM johnsod6@ohio.edu RI Johnson, Daniel/E-6789-2011; Meinzer, Frederick/C-3496-2012 OI Johnson, Daniel/0000-0001-5890-3147; FU NSF [IOS-0919871, NSF EAR 07-25019] FX This research was supported by NSF IOS-0919871 and NSF EAR 07-25019. NR 49 TC 66 Z9 73 U1 7 U2 73 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2011 VL 31 IS 6 BP 659 EP 668 DI 10.1093/treephys/tpr050 PG 10 WC Forestry SC Forestry GA 796UA UT WOS:000293076900010 PM 21724585 ER PT J AU Ferguson, TL Rude, BJ King, DT AF Ferguson, Treena L. Rude, Brian J. King, D. Tommy TI Nutrient Utilization and Diet Preference of American White Pelicans Consuming Either a Mono- or Multi-Species Diet SO WATERBIRDS LA English DT Article DE American White Pelican; carp; catfish; diet; metabolism; Pelecanus erythrorhynchos; preference ID DOUBLE-CRESTED CORMORANTS; ENERGY-REQUIREMENTS; PELECANUS-ONOCROTALUS; FISH; DIGESTIBILITY; AQUACULTURE AB American White Pelicans (Pelecanus erythrorhynchos) are reported to consume large quantities of commercial catfish in Alabama, Arkansas, Louisiana and Mississippi, negatively impacting aquaculture production. If pelicans consume a single item diet, such as catfish, nutritional deficiencies may result. Therefore, effects of mono- and multi-species diets were examined to determine nutritional impacts on pelicans in addition to determining if preferences for certain fish species exist. Twelve American White Pelicans captured in northwest Mississippi were used for a seven day metabolism trial followed by a two day preference trial. In the metabolism trial, pelicans were assigned to one of three treatment diets (four birds/diet): catfish only, carp only, or both (50% catfish and 50% carp). Pelicans consuming only catfish metabolized less thy matter, organic matter and energy than those consuming only carp or both. Four pelicans were used to determine preference for carp or catfish. Pelicans ate more (P = 0.001) carp (89% of diet) and digested nutrients from carp more efficiently than they did from catfish. Pelicans can meet nutritional requirements by consuming a mono-species diet of fish; however, certain prey may be more beneficial and even preferred for consumption. Received 15 November 2010, accepted 5 April 2011. C1 [King, D. Tommy] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Mississippi State, MS 39762 USA. [Ferguson, Treena L.; Rude, Brian J.] Mississippi State Univ, Dept Anim & Dairy Sci, Mississippi State, MS 39762 USA. RP King, DT (reprint author), USDA, Wildlife Serv, Natl Wildlife Res Ctr, Mississippi State, MS 39762 USA. EM Tommy.King@aphis.usda.gov NR 25 TC 1 Z9 1 U1 4 U2 15 PU WATERBIRD SOC PI WASHINGTON PA NATL MUSEUM NATURAL HISTORY SMITHSONIAN INST, WASHINGTON, DC 20560 USA SN 1524-4695 J9 WATERBIRDS JI Waterbirds PD JUN PY 2011 VL 34 IS 2 BP 218 EP 224 PG 7 WC Ornithology SC Zoology GA 797WH UT WOS:000293160300011 ER PT J AU Walter, WD Baasch, DM Hygnstrom, SE Trindle, BD Tyre, AJ Millspaugh, JJ Frost, CJ Boner, JR VerCauteren, KC AF Walter, W. David Baasch, David M. Hygnstrom, Scott E. Trindle, Bruce D. Tyre, Andrew J. Millspaugh, Joshua J. Frost, Charles J. Boner, Justin R. VerCauteren, Kurt C. TI Space use of sympatric deer in a riparian ecosystem in an area where chronic wasting disease is endemic SO WILDLIFE BIOLOGY LA English DT Article DE chronic wasting disease; home range; movements; mule deer; Odocoileus hemionus; O. virginianus; resource selection; space use; sympatry; white-tailed deer ID WHITE-TAILED DEER; HOME-RANGE SIZE; MULE DEER; HABITAT SELECTION; RESOURCE SELECTION; CONTACT RATES; PRION DISEASE; PATTERNS; EPIDEMIOLOGY; MIGRATION AB Knowledge of movements, range size and spatial overlap of sympatric deer is integral to understanding chronic wasting disease (CWD) in endemic areas and can assist resource managers in modeling the spread of the disease. We radio-collared 70 deer (30 mule deer Odocoileus hemionus and 40 white-tailed deer O. virginianus) in the North Platte River Valley in western Nebraska, USA, from 2004 to 2007 to document movements, size and spatial overlap of home range and resource selection of these sympatric species of deer. We compared home-range size and overlap and resource selection among male and female mule deer and white-tailed deer to examine relative use of space in order to understand the potential for indirect spread of CWD better. We identified forested, riparian habitats as high selection of use and these habitats could likely contribute to the potential spreading of CWD between sympatric deer that concentrate in these areas. We found that migration, size of home range of female mule deer, and similarities in resource selection could contribute to sustaining or spreading CWD in Nebraska. The role of female mule deer in the spread of CWD coupled with selection for riparian corridors by both species should be investigated further, because these factors may be a primary determinant of disease spread in the historical range of mule deer. C1 [Walter, W. David; VerCauteren, Kurt C.] Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Baasch, David M.; Hygnstrom, Scott E.; Tyre, Andrew J.; Frost, Charles J.; Boner, Justin R.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Trindle, Bruce D.] Nebraska Game & Parks Commiss, Norfolk, NE 68701 USA. [Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. RP Walter, WD (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM WDWalter@aphis.usda.gov; baaschd@headwaterscorp.org; shygnstr@unlnotes.edu; bruce.trindle@nebraska.gov; atyre2@unl.edu; MillspaughJ@missouri.edu; charlesf@alaskasealife.org; juboner@yahoo.com; Kurt.C.VerCauteren@aphis.usda.gov FU USDA-APHIS-WS-National Wildlife Research Center; Nebraska Game and Parks Commission; U.S. Geological Survey; Berryman Institute-East; Boone and Crockett Club; Nebraska Bowhunter's Association; Cabelas Inc.; University of Nebraska-Lincoln FX support for this research was provided by the USDA-APHIS-WS-National Wildlife Research Center, Nebraska Game and Parks Commission, U.S. Geological Survey, Berryman Institute-East, Boone and Crockett Club, Nebraska Bowhunter's Association, Cabelas Inc. and the University of Nebraska-Lincoln. Special thanks is given to technicians and volunteers who assisted in capturing deer and collecting data, especially G.M. Clements, M. Clements, J.M. Gilsdorf, T. Kinsell, S. Korte, C. Luedtke, B. Malochek and R. Otto. NR 75 TC 4 Z9 5 U1 3 U2 11 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 J9 WILDLIFE BIOL JI Wildlife Biol. PD JUN PY 2011 VL 17 IS 2 BP 191 EP 209 DI 10.2981/10-055 PG 19 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 799DG UT WOS:000293263100008 ER PT J AU Ooi, EMM Watts, GF Barrett, PHR AF Ooi, Esther M. M. Watts, Gerald F. Barrett, P. Hugh R. TI Altered metabolism of apolipoprotein C-III a contributor in chronic kidney disease? SO CLINICAL LIPIDOLOGY LA English DT Editorial Material DE apoC-III; cardiovascular disease; kidney disease; kinetics; lipoprotein metabolism ID LOW-DENSITY-LIPOPROTEIN; CORONARY-ARTERY-DISEASE; CARDIOVASCULAR RISK; ENDOTHELIAL-CELLS; DIALYSIS PATIENTS; POTENTIAL MECHANISM; PERITONEAL-DIALYSIS; TRIGLYCERIDE LEVELS; MONOCYTIC CELLS; A-I C1 [Watts, Gerald F.; Barrett, P. Hugh R.] Univ Western Australia, Metab Res Ctr, Sch Med & Pharmacol, Royal Perth Hosp, Perth, WA 6009, Australia. [Ooi, Esther M. M.] Tufts Univ, Lipid Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Barrett, PHR (reprint author), Univ Western Australia, Metab Res Ctr, Sch Med & Pharmacol, Royal Perth Hosp, Perth, WA 6009, Australia. EM hugh.barrett@uwa.edu.au RI Barrett, Hugh/B-2745-2011; Ooi, Esther/B-3257-2011; OI Ooi, Esther/0000-0002-2097-633X; Barrett, Peter Hugh/0000-0003-3223-6125 NR 45 TC 0 Z9 0 U1 0 U2 0 PU FUTURE MEDICINE LTD PI LONDON PA UNITEC HOUSE, 3RD FLOOR, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON, N3 1QB, ENGLAND SN 1758-4299 J9 CLIN LIPIDOL JI Clin. Lipidol. PD JUN PY 2011 VL 6 IS 3 BP 247 EP 251 DI 10.2217/CLP.11.18 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 787DB UT WOS:000292355700001 ER PT J AU Gibson, DM King, BC Hayes, ML Bergstrom, GC AF Gibson, Donna M. King, Brian C. Hayes, Marshall L. Bergstrom, Gary C. TI Plant pathogens as a source of diverse enzymes for lignocellulose digestion SO CURRENT OPINION IN MICROBIOLOGY LA English DT Review ID WALL-DEGRADING ENZYMES; CELL-WALL; FUSARIUM-GRAMINEARUM; ENZYMATIC-HYDROLYSIS; BOTRYTIS-CINEREA; GENOMIC ANALYSIS; FUNGI; BIOMASS; GENE; DEGRADATION AB The plant cell wall is a major barrier that many plant pathogens must surmount for successful invasion of their plant hosts. Full genome sequencing of a number of plant pathogens has revealed often large, complex, and redundant enzyme systems for degradation of plant cell walls. Recent surveys have noted that plant pathogenic fungi are highly competent producers of lignocellulolytic enzymes, and their enzyme activity patterns reflect host specificity. We propose that plant pathogens may contribute to biofuel production as diverse sources of accessory enzymes for more efficient conversion of lignocellulose into fermentable sugars. C1 [Gibson, Donna M.] ARS, Biol Integrated Pest Management Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. [King, Brian C.; Hayes, Marshall L.; Bergstrom, Gary C.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Gibson, DM (reprint author), ARS, Biol Integrated Pest Management Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Tower Rd, Ithaca, NY 14853 USA. EM Donna.Gibson@ars.usda.gov RI King, Brian/K-2021-2016 OI King, Brian/0000-0003-3954-5190 FU US Department of Energy, Northeast Sun Grant; USDA, NIFA; National Institutes of Health (NIGMS) FX We acknowledge our lab members for their assistance in conducting the studies described in King et al. (2011), and referenced in this review (Ref. 129*]). This work was supported by grants from the US Department of Energy, Northeast Sun Grant, and USDA, NIFA. Additional support for Brian C. King was provided by a Chemistry-Biology Interface training grant from the National Institutes of Health (NIGMS). Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 50 TC 24 Z9 24 U1 2 U2 26 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5274 J9 CURR OPIN MICROBIOL JI Curr. Opin. Microbiol. PD JUN PY 2011 VL 14 IS 3 SI SI BP 264 EP 270 DI 10.1016/j.mib.2011.04.002 PG 7 WC Microbiology SC Microbiology GA 795CE UT WOS:000292948300007 PM 21536481 ER PT J AU Zhang, Y Simsek, S Campanella, OH Ohm, JB Chang, H Reuhs, BL Mergoum, M AF Zhang, Yu Simsek, Senay Campanella, Osvaldo H. Ohm, Jae B. Chang, Hector Reuhs, Bradley L. Mergoum, Mohamed TI RHEOLOGICAL CHANGES IN REFRIGERATED DOUGH DURING STORAGE IN RELATION TO PROTEINS SO JOURNAL OF FOOD PROCESS ENGINEERING LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SIZE-EXCLUSION HPLC; WHEAT-FLOUR DOUGH; BREADMAKING; QUALITY; GLUTEN; ARABINOXYLANS; FRACTIONS; STARCH AB Refrigerated dough is a flour-based, unbaked product that is stored between 4 and 7C. The aim of this work was to study the rheological properties of refrigerated dough during storage and determine their correlations with dough proteins. Rheological properties were determined using texture analyzer and dynamic oscillatory rheometry during 34 days of storage. The protein analysis was performed by size-exclusion high performance liquid chromatography. On day 34, R(max) was 93.8% higher than day 0. Both, the G' and G" moduli decreased during storage. Dough exhibited the major decreases on the moduli on day 3 and day 16. By comparing the viscoelastic properties of day 0 and day 16, a 50% decrease on the elastic modulus and a roughly 30% decrease in the loss modulus were observed. Changes in the protein fractions of dough samples were related to their rheological properties. The high and low molecular weight polymeric protein and gliadin were positively correlated to dough extensibility (r > 0.8343). C1 [Zhang, Yu; Simsek, Senay; Mergoum, Mohamed] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Campanella, Osvaldo H.; Chang, Hector] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Campanella, Osvaldo H.; Chang, Hector; Reuhs, Bradley L.] Purdue Univ, Whistler Ctr Carbohydrate Res, W Lafayette, IN 47907 USA. [Ohm, Jae B.] N Dakota State Univ, USDA ARS RRVARC NCSL, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58105 USA. [Reuhs, Bradley L.] Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA. RP Simsek, S (reprint author), N Dakota State Univ, Dept Plant Sci, POB 6050,Dept 7670, Fargo, ND 58108 USA. EM senay.simsek@ndsu.edu RI Mergoum, Mohamed/D-3815-2014; OI Campanella, Osvaldo/0000-0002-2281-5185 FU North Dakota Agricultural Product Utilization Commission [FAR0013155]; North Dakota State University Agricultural Experiment Station FX This work was supported by Grant No. FAR0013155 from the North Dakota Agricultural Product Utilization Commission and North Dakota State University Agricultural Experiment Station. We would like to thank Kristin Whitney for her help in preparation of the dough samples and Angelo Ostenson for her help during analysis of proteins. NR 30 TC 4 Z9 4 U1 5 U2 12 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0145-8876 J9 J FOOD PROCESS ENG JI J. Food Process Eng. PD JUN PY 2011 VL 34 IS 3 BP 639 EP 656 DI 10.1111/j.1745-4530.2009.00415.x PG 18 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 795OT UT WOS:000292984200007 ER PT J AU Petrice, TR Haack, RA AF Petrice, Toby R. Haack, Robert A. TI Effects of Cutting Time, Stump Height, and Herbicide Application on Ash (Fraxinus Spp.) Stump Sprouting and Colonization by Emerald Ash Borer (Agrilus planipennis) SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE Buprestidae; control; coppice; eradication; triclopyr ID TWOLINED CHESTNUT BORER; BETULA-PUBESCENS; NORTH-AMERICA; FELLING TIME; RED ALDER; COLEOPTERA; BUPRESTIDAE; TREES; CUT; ATTRACTION AB Efforts to eradicate or slow the spread of emerald ash borer (EAB) (Agrilus planipennis Fairmaire [Coleoptera: Buprestidae]) include cutting infested and nearby uninfested ash (Fraxinus spp.) trees. However, ash trees readily sprout after they have been cut, providing potential host material for EAB. In 2004-2005, we conducted studies to determine how different cutting times (midspring, late spring, and late summer), different cutting heights (0-5, 10-15, and 20-25 cm above the ground), and triclopyr (44% active ingredient) stump treatment of green ash (Fraxinus pennsylvanica Marsh.) trees affected subsequent stump sprouting and colonization by EAB. We also cut white ash (Fraxinus americana L.) and black ash (Fraxinus nigra Marsh.) trees 20-25 cm above the ground in late spring. Some stumps of each ash species tested sprouted and were colonized by EAB. All green ash stumps treated with triclopyr died and were not colonized by EAB. Stump sprouting was significantly lower for stumps cut in late spring compared with stumps cut in midspring or late summer. Stump sprouting did not vary significantly among cutting heights. None of the green ash stumps cut in midspring or cut 0-5 cm above the ground were colonized by EAB; however, the frequency of stump colonization by EAB did not vary significantly among cutting times or cutting heights. C1 [Petrice, Toby R.; Haack, Robert A.] US Forest Serv, E Lansing, MI 48823 USA. RP Petrice, TR (reprint author), US Forest Serv, 1407 S Harrison Rd, E Lansing, MI 48823 USA. EM tpetrice@fs.fed.us NR 28 TC 2 Z9 4 U1 1 U2 18 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 EI 1938-3762 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2011 VL 28 IS 2 BP 79 EP 83 PG 5 WC Forestry SC Forestry GA 796WM UT WOS:000293084200004 ER PT J AU Weiskittel, AR Kenefic, LS Li, RX Brissette, J AF Weiskittel, Aaron R. Kenefic, Laura S. Li, Rongxia Brissette, John TI Stand Structure and Composition 32 Years after Precommercial Thinning Treatments in a Mixed Northern Conifer Stand in Central Maine SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE balsam fir; red spruce; crop tree release; Acadian Forest; Penobscot Experimental Forest ID BALSAM FIR STANDS; RED SPRUCE; GROWTH; YIELD AB The effects of four precommercial thinning (PCT) treatments on an even-aged northern conifer stand in Maine were investigated by examining stand structure and composition 32 years after treatment. Replicated treatments applied in 1976 included: (1) control (no PCT), (2) row thinning (rowthin; 5-ft-wide row removal with 3-ft-wide residual strips), (3) row thinning with crop tree release (rowthin + CTR; 5-ft-wide row removal with crop tree release at 8-ft intervals in 3-ft-wide residual strips), and (4) crop tree release (CTR; release of selected crop trees at 8 x 8-ft intervals). PCT plots had more large trees and fewer small trees than the control in 2008. There were no other significant differences between the rowthin and control. The rowthin + CTR and CTR treatments had lower total and hardwood basal area (BA) and higher merchantable conifer BA than the control. CTR also resulted in more red spruce (Picea rubens [Sarg.]) and less balsam fir (Abies balsamea [L.]) than the other treatments. Although stand structures for rowthin + CTR and CTR were similar, the percentage of spruce in CTR was greater. Although the less-intensive rowthin + CTR treatment may provide many of the same benefits as CTR, the latter would be the preferred treatment if increasing the spruce component of a stand is an objective. Overall, early thinning treatments were found to have long-term effects on key stand attributes, even more than 30 years after treatment in areas with mixed species composition and moderate site potential. C1 [Weiskittel, Aaron R.; Li, Rongxia] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Kenefic, Laura S.] US Forest Serv, No Res Sect, Bradley, ME 04411 USA. [Brissette, John] US Forest Serv, No Res Sect, Durham, NH 03824 USA. RP Weiskittel, AR (reprint author), Univ Maine, Sch Forest Resources, 5755 Nutting Hall, Orono, ME 04469 USA. EM aaron.weiskittel@maine.edu RI Kenefic, Laura/H-3177-2014 FU University of Maine School of Forest Resources Forest Bioproducts Research Initiative; US Forest Service, Northern Research Station FX Aaron R. Weiskittel (aaron.weiskittel@maine.edu), School of Forest Resources, University of Maine, 5755 Nutting Hall, Orono, ME 04469. Laura S. Kenefic, US Forest Service, Northern Research Section, Bradley, ME 04411. John Brissette, US Forest Service, Northern Research Section, Durham, NII 03824. Rongxia Li, School of Forest Resources, University of Maine, 5755 Nutting Hall, Orono, ME 04469. The US Forest Service, Northern Research Station, provided data and access to the study sites. Richard Dionne, Jacob Hicks, Karl Buckley, Baburam Rijal, Peter McBride, Alexandria Small, and Matthew Russell assisted with fieldwork. This work was funded by the University of Maine School of Forest Resources Forest Bioproducts Research Initiative and the US Forest Service, Northern Research Station. NR 22 TC 4 Z9 4 U1 1 U2 7 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 EI 1938-3762 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2011 VL 28 IS 2 BP 92 EP 96 PG 5 WC Forestry SC Forestry GA 796WM UT WOS:000293084200006 ER PT J AU Evans, DM Aust, WM Dolloff, CA Templeton, BS Peterson, JA AF Evans, Daniel M. Aust, W. Michael Dolloff, C. Andrew Templeton, Ben S. Peterson, John A. TI Eastern Hemlock Decline in Riparian Areas from Maine to Alabama SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE eastern hemlock; hemlock woolly adelgid; forest insect pests; forest disturbance; shrub competition ID WOOLLY ADELGID HOMOPTERA; SOUTHERN NEW-ENGLAND; LITTER DECOMPOSITION; LOW-TEMPERATURES; FOREST RESPONSE; CONSEQUENCES; ECOSYSTEMS; NITROGEN; INFESTATION; MORTALITY AB Eastern hemlock (Tsuga canadensis) in the Appalachian mountain range is threatened by the introduced hemlock woolly adelgid (Adelges tsugae). Potential impacts on riparian systems are great because of eastern hemlock's role as a foundation species that influences site soil, vegetation, and stream characteristics. We installed permanent research sites at 49 locations in riparian areas, from Maine to Alabama, to survey eastern hemlock health, measure stand dynamics, and predict near-term forest composition without eastern hemlock. This report summarizes the initial stand measurements from summer of 2008. We found hemlock woolly adelgid present at 25 of 49 stands from Massachusetts to Georgia, and all of these stands had some degree of hemlock decline. New England states, Ohio, western Pennsylvania, Kentucky, and Alabama had good hemlock health and no sign of hemlock woolly adelgid. Eighteen of the 49 sites had no nonhemlock conifer species in the overstory, and 30 of 49 sites had less than 5 m(2) ha(-1) of nonhemlock conifers. Without eastern hemlock, 25 of the stands would have more than 90% hardwood in the overstory, many of which are in the mid-Atlantic and southern states at sites dominated by shrubs in the understory such as Rhododendron maximum. Competition from shrubs may hinder stand regeneration after disturbance by hemlock woolly adelgid. On the basis of the abundance of hardwood species and lack of conifer species present in the overstory at many infested hemlock-dominated stands, these sites may convert to hardwood-dominated stands, which will affect terrestrial and aquatic ecosystem dynamics. C1 [Evans, Daniel M.; Aust, W. Michael; Templeton, Ben S.; Peterson, John A.] Virginia Polytech Inst & State Univ, Forest Resources & Environm Conservat Dept, Blacksburg, VA 24060 USA. [Dolloff, C. Andrew] US Forest Serv, So Res Stn, Blacksburg, VA 24060 USA. RP Evans, DM (reprint author), Virginia Polytech Inst & State Univ, Forest Resources & Environm Conservat Dept, 228 Cheatham Hall, Blacksburg, VA 24060 USA. EM daniel76@vt.edu FU US Fish and Wildlife Service; US Forest Service; US National Park Service; Pennsylvania State Parks; Ohio Division of Forestry; Massachusetts Department of Conservation and Recreation; New York Department of Environmental Conservation; New Jersey Department of Parks and Forestry FX Daniel M. Evans (daniel76@vt.edu), W. Michael Aust, Ben S. Templeton, and John A. Peterson, Forest Resources and Environmental Conservation Department, Virginia Polytechnic Institute and State University, 228 Cheatham Hall, Blacksburg, VA 24060. C. Andrew Dolloff, US Forest Service, Southern Research Station, 1710 Ramble Rd., Blacksburg, VA 24060. Access to research sites was granted by the US Fish and Wildlife Service, US Forest Service, US National Park Service, Pennsylvania State Parks, Ohio Division of Forestry, Massachusetts Department of Conservation and Recreation, New York Department of Environmental Conservation, and New Jersey Department of Parks and Forestry. Field assistance was provided by Drew Stephenson, and editing assistance was provided by Amy Villamagna. The quality of this report has been greatly improved through the suggestions of three anonymous reviewers. NR 33 TC 6 Z9 6 U1 1 U2 32 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 EI 1938-3762 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2011 VL 28 IS 2 BP 97 EP 104 PG 8 WC Forestry SC Forestry GA 796WM UT WOS:000293084200007 ER PT J AU Kalkan, H Beriat, P Yardimci, Y Pearson, TC AF Kalkan, H. Beriat, P. Yardimci, Y. Pearson, T. C. TI Detection of contaminated hazelnuts and ground red chili pepper flakes by multispectral imaging SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Multispectral imaging; Food safety; Feature extraction; Classification ID PISTACHIO NUTS; AFLATOXIN; FLUORESCENCE; CLASSIFICATION AB Mycotoxins are the toxic metabolites of certain filamentous fungi and have been demonstrated to cause various health problems in humans, including immunosuppression and cancer. Among them, the aflatoxins have received greater attention because they are potent carcinogens and are responsible for many human deaths per annum, mostly in non-industrialized countries. Various regulatory agencies have enforced limits on the concentrations of these toxins in foods and feeds involved in international commerce. Hyperspectral and multispectral imaging are becoming increasingly important for rapid and non-destructive testing for the presence of such contaminants. However, the high number of spectral bands needed may render such image acquisition systems too complex, expensive and slow. Moreover, they tend to generate overwhelming amount of data, making effective processing of this information in real time difficult. In this study, a two-dimensional local discriminant bases algorithm was developed to detect the location of the discriminative features in the multispectral data space. The algorithm identifies the optimal passband width and center frequencies of optical filters to be used for a multispectral imaging system. This was applied to a multispectral imaging system used to detect aflatoxin-contaminated hazelnut kernels and red chili peppers. Classification accuracies of 92.3% and 80% were achieved for aflatoxin-contaminated and uncontaminated hazelnuts and red chili peppers, respectively. The aflatoxin concentrations were decreased from 608 to 0.84 ppb for tested hazelnuts and from 38.26 to 22.85 ppb for red chili peppers by removal of the nuts/peppers that were classified as aflatoxin-contaminated. The algorithm was also used to classify fungal contaminated and uncontaminated hazelnut kernels, and an accuracy of 95.6% was achieved for this broader classification. (C) 2011 Elsevier B.V. All rights reserved. C1 [Kalkan, H.] Suleyman Demirel Univ, TR-32200 Isparta, Turkey. [Kalkan, H.; Beriat, P.; Yardimci, Y.] Middle E Tech Univ, TR-06531 Ankara, Turkey. [Pearson, T. C.] USDA ARS CGAHR, Manhattan, KS USA. RP Kalkan, H (reprint author), Suleyman Demirel Univ, TR-32200 Isparta, Turkey. EM habilkalkan@sdu.edu.tr FU National Scientific Research Council of Turkey (TUBITAK) [EEEAG-106E057] FX This work is supported by the National Scientific Research Council of Turkey (TUBITAK) project EEEAG-106E057. NR 28 TC 25 Z9 26 U1 1 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2011 VL 77 IS 1 BP 28 EP 34 DI 10.1016/j.compag.2011.03.005 PG 7 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 791ZY UT WOS:000292711500004 ER PT J AU McMaster, GS Edmunds, DA Wilhelm, WW Nielsen, DC Prasad, PVV Ascough, JC AF McMaster, Gregory S. Edmunds, D. A. Wilhelm, W. W. Nielsen, D. C. Prasad, P. V. V. Ascough, J. C., II TI PhenologyMMS: A program to simulate crop phenological responses to water stress SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Simulation model; Decision support systems; Crop development; Growth stages; Crop management; Seedling emergence ID WINTER-WHEAT PHENOLOGY; CENTRAL GREAT-PLAINS; MODEL; GROWTH; TEMPERATURE; SYSTEMS; SOIL; NITROGEN; APSIM AB Crop phenology is fundamental for understanding crop growth and development, and increasingly influences many agricultural management practices. Water deficits are one environmental factor that can influence crop phenology through shortening or lengthening the developmental phase, yet the phenological responses to water deficits have rarely been quantified. The objective of this paper is to provide an overview of a decision support technology software tool, PhenologyMMS V1.2, developed to simulate the phenology of various crops for varying levels of soil water. The program is intended to be simple to use, requires minimal information for calibration, and can be incorporated into other crop simulation models. It consists of a Java interface connected to FORTRAN science modules to simulate phenological responses. The complete developmental sequence of the shoot apex correlated with phenological events, and the response to soil water availability for winter and spring wheat (Triticum aestivum L), winter and spring barley (Hordeum vulgare L.), corn (Zen mays L), sorghum (Sorghum bicolor L.), proso millet (Panicum milaceum L), hay/foxtail millet [Seraria italica (L) P. Beauv.], and sunflower (Helianthus annus L) were created based on experimental data and the literature. Model evaluation consisted of testing algorithms using "generic" default phenology parameters for wheat (i.e., no calibration for specific cultivars was used) for a variety of field experiments to predict developmental events. Results demonstrated that the program has general applicability for predicting crop phenology and can aid in crop management. Published by Elsevier B.V. C1 [McMaster, Gregory S.; Edmunds, D. A.; Ascough, J. C., II] USDA ARS NPA, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Wilhelm, W. W.] Univ Nebraska, USDA ARS NPA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Nielsen, D. C.] USDA ARS NPA, Cent Plains Resource Management Unit, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Prasad, P. V. V.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. RP McMaster, GS (reprint author), USDA ARS NPA, Agr Syst Res Unit, 2150 Ctr Ave,Bldg D,Suite 200, Ft Collins, CO 80526 USA. EM Greg.McMaster@ars.usda.gov RI Prasad, P.V. Vara/B-3835-2012 OI Prasad, P.V. Vara/0000-0001-6632-3361 NR 27 TC 11 Z9 11 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2011 VL 77 IS 1 BP 118 EP 125 DI 10.1016/j.compag.2011.04.003 PG 8 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 791ZY UT WOS:000292711500015 ER PT J AU Dror, DK Allen, LH AF Dror, Daphna K. Allen, Lindsay H. TI Vitamin E deficiency in developing countries SO FOOD AND NUTRITION BULLETIN LA English DT Article DE Deficiency; developing country; oxidative stress; vitamin E ID PROTEIN-ENERGY MALNUTRITION; RANDOMIZED CONTROLLED-TRIAL; PLASMA ALPHA-TOCOPHEROL; GAMMA-TOCOPHEROL; OXIDATIVE STRESS; BETA-CAROTENE; NEUROLOGICAL DEFICITS; SERUM CONCENTRATIONS; MICRONUTRIENT STATUS; FALCIPARUM-MALARIA AB In addition to its role as a potent antioxidant, vitamin E is involved in a wide range of physiological processes, ranging from immune function and control of inflammation to regulation of gene expression and cognitive performance. Results from multiple studies suggest that poor nutritional status and higher prevalence of other oxidative stressors such as malaria and HIV infection predispose populations in developing countries for vitamin E deficiency. Although direct comparison between study outcomes is complicated by varied definitions of vitamin E deficiency, data trends indicate that children and the elderly are more vulnerable age groups and that men may be at higher risk for deficiency than women. Public health initiatives aimed at improving the vitamin E status of high-risk populations in developing countries would be prudent to counteract oxidative stress, improve immune function, and protect against neurologic and cognitive deficits. Additional research is needed to establish dose-response relationships of various interventions and to develop cost-effective, culturally-appropriate, and targeted programs. C1 [Dror, Daphna K.; Allen, Lindsay H.] ARS, Allen Lab, USDA, WHNRC, Davis, CA 95616 USA. RP Dror, DK (reprint author), ARS, Allen Lab, USDA, WHNRC, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM dkdror@ucdavis.edu FU HarvestPlus FX Financial support for this review was provided by HarvestPlus (www.HarvestPlus.org), a global alliance of agriculture and nutrition institutions working to increase the micronutrient contents of staple food crops through biofortification. The views expressed do not necessarily reflect those of HarvestPlus. NR 105 TC 19 Z9 20 U1 1 U2 12 PU INT NUTRITION FOUNDATION PI BOSTON PA 150 HARRISON AVE, BOSTON, MA 02111 USA SN 0379-5721 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2011 VL 32 IS 2 BP 124 EP 143 PG 20 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 791NL UT WOS:000292671800006 PM 22164974 ER PT J AU Calkin, DC Finney, MA Ager, AA Thompson, MP Gebert, KM AF Calkin, David C. Finney, Mark A. Ager, Alan A. Thompson, Matthew P. Gebert, Krista M. TI Progress towards and barriers to implementation of a risk framework for US federal wildland fire policy and decision making SO FOREST POLICY AND ECONOMICS LA English DT Article DE Wildfire; Risk management; Decision support; Wildfire policy ID FUEL TREATMENT; LARGE WILDFIRES; UNITED-STATES; MANAGEMENT; LANDSCAPE; FOREST; MODEL; TERM; OPTIMIZATION; SIMULATION AB In this paper we review progress towards the implementation of a risk management framework for US federal wildland fire policy and operations. We first describe new developments in wildfire simulation technology that catalyzed the development of risk-based decision support systems for strategic wildfire management. These systems include new analytical methods to measure wildfire risk to human and ecological values and to inform fuel treatment investment strategies at national, regional, and local scales. Application of the risk management framework to support wildfire incidents has been dramatically advanced with the Wildland Fire Decision Support System and allowed policy modifications that encourage management of incidents for multiple objectives. The new wildfire risk management decision support systems we discuss provide Federal agencies in the US the ability to integrate risk-informed approaches to a wide range of wildfire management responsibilities and decisions. While much progress has been made, there remain several barriers that need to be addressed to fully integrate risk science into current wildfire management practices. We conclude by identifying five primary issues that if properly addressed could help public land management better realize the opportunities and potential payoffs from fully adopting a risk management paradigm. Published by Elsevier B.V. C1 [Calkin, David C.; Finney, Mark A.; Thompson, Matthew P.; Gebert, Krista M.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59807 USA. [Ager, Alan A.] US Forest Serv, Western Wildland Environm Threat Assessment Ctr, USDA, Prineville, OR 97754 USA. RP Calkin, DC (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, POB 7669, Missoula, MT 59807 USA. EM decalkin@fs.fed.us RI Calkin, David/F-4727-2014 NR 84 TC 28 Z9 32 U1 3 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 J9 FOREST POLICY ECON JI Forest Policy Econ. PD JUN PY 2011 VL 13 IS 5 BP 378 EP 389 DI 10.1016/j.forpol.2011.02.007 PG 12 WC Forestry SC Forestry GA 789RJ UT WOS:000292533700009 ER PT J AU Moreno, JP Kelley, ML Landry, DN Paasch, V Terlecki, MA Johnston, CA Foreyt, JP AF Moreno, Jennette Palcic Kelley, Mary Lou Landry, David N. Paasch, Valerie Terlecki, Meredith A. Johnston, Craig A. Foreyt, John P. TI Development and validation of the Family Health Behavior Scale SO INTERNATIONAL JOURNAL OF PEDIATRIC OBESITY LA English DT Article DE Obesity; assessment; eating; nutrition; physical activity; family; child ID PHYSICAL-ACTIVITY; CHILDHOOD OBESITY; CHILDREN; PARENTS; ADOLESCENTS; PREVALENCE; PATTERNS; EXERCISE; ADULTS; MASS AB Objective. The purpose of the current study was to develop a psychometrically sound, parent-report measure of family and child behaviors related to obesity in children between 5- and 12-years-old. Methods. Item generation, item selection, and initial exploratory factor analysis yielded a 27-item measure called the Family Health Behavior Scale (FHBS). Results. The FHBS contains four subscales measuring health-promoting family behaviors, obesogenic behaviors, meal-time routines, and family physical activity habits. Parent ratings on the FHBS were shown to predict their child's weight classification. The FHBS also demonstrated adequate internal consistency and temporal stability. Conclusions. The results of the study suggest that the FHBS is a promising measure of family eating and physical activity habits related to obesity in children. C1 [Moreno, Jennette Palcic; Landry, David N.; Johnston, Craig A.; Foreyt, John P.] Baylor Coll Med, Dept Pediat Nutr, Houston, TX 77030 USA. [Moreno, Jennette Palcic; Johnston, Craig A.; Foreyt, John P.] ARS, USDA, Childrens Nutr Res Ctr, Houston, TX USA. [Kelley, Mary Lou; Landry, David N.; Paasch, Valerie; Terlecki, Meredith A.] Louisiana State Univ, Dept Psychol, Baton Rouge, LA 70803 USA. RP Moreno, JP (reprint author), Baylor Coll Med, Dept Pediat Nutr, 6655 Travis Mailstop 320, Houston, TX 77030 USA. EM palcic@bcm.edu OI Terlecki, Meredith/0000-0002-4726-6653 NR 30 TC 1 Z9 1 U1 1 U2 9 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 1747-7166 J9 INT J PEDIATR OBES JI Int. J. Pediatr. Obes. PD JUN PY 2011 VL 6 IS 2-2 BP E480 EP E486 DI 10.3109/17477166.2011.575148 PG 7 WC Pediatrics SC Pediatrics GA 791YA UT WOS:000292704500059 PM 21615229 ER PT J AU Geiger, JH Pratt, PD Wheeler, GS Williams, DA AF Geiger, John H. Pratt, Paul D. Wheeler, Gregory S. Williams, Dean A. TI HYBRID VIGOR FOR THE INVASIVE EXOTIC BRAZILIAN PEPPERTREE (SCHINUS TEREBINTHIFOLIUS RADDI., ANACARDIACEAE) IN FLORIDA SO INTERNATIONAL JOURNAL OF PLANT SCIENCES LA English DT Article DE invasive species; Schinus terebinthifolius; multiple introductions; hybridization; common garden experiment; hybrid vigor; intraspecific ID GENETIC-VARIATION; PLANT INVASIONS; EVOLUTION; HYBRIDIZATION; BIOLOGY; ABILITY; COLONIZATION; CHLOROPLAST; RECRUITMENT; GERMINATION AB How can successful invaders overcome reduced genetic variation via small founder population sizes to persist, thrive, and successfully adapt to a new set of environmental conditions? An expanding body of literature posits hybridization, both inter- and intraspecific, as a driver of the evolution of invasiveness via genetic processes. We studied Brazilian peppertree (Schinus terebinthifolius), a tree species native to South America that is a successful invader throughout Florida. The tree was introduced separately to the east and west coasts of Florida more than 100 years ago from genetically distinct source populations. We conducted a common garden experiment to compare the early life-stage performance of hybrids versus their progenitors. We hypothesized that hybrids would outperform their progenitors due to the positive genetic effects of intraspecific hybridization (i.e., hybrid vigor). Hybrid seeds germinated at higher rates than eastern seeds. Over the 8-mo experiment, a greater proportion of hybrid seedlings survived than did western seedlings, and hybrids attained greater biomass than the western types. The cumulative hybrid advantage of both seed germination and seedling survival led to the establishment of nearly 45% more hybrid seedlings versus either progenitor. Documenting fitness advantages for hybrids over their progenitors is a requisite finding to consider hybridization as a factor in the success of invasive species. C1 [Geiger, John H.; Pratt, Paul D.; Wheeler, Gregory S.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Williams, Dean A.] Texas Christian Univ, Dept Biol, Ft Worth, TX 76129 USA. RP Geiger, JH (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM john.geiger@ars.usda.gov; paul.pratt@ars.usda.gov; greg.wheeler@ars.usda.gov; dean.williams@tcu.edu RI Williams, Dean/H-6108-2016 OI Williams, Dean/0000-0002-9001-6019 NR 42 TC 15 Z9 15 U1 4 U2 34 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 1058-5893 J9 INT J PLANT SCI JI Int. J. Plant Sci. PD JUN PY 2011 VL 172 IS 5 BP 655 EP 663 DI 10.1086/659457 PG 9 WC Plant Sciences SC Plant Sciences GA 791XV UT WOS:000292703800004 ER PT J AU Polacco, JC Hyten, DL Medeiros-Silva, M Sleper, DA Bilyeu, KD AF Polacco, Joe C. Hyten, David L. Medeiros-Silva, Monica Sleper, David A. Bilyeu, Kristin D. TI Mutational analysis of the major soybean UreF paralogue involved in urease activation SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Eu2; Nickel; SNP; soybean; urease; UreF; Universal Soy Linkage Panel ID UBIQUITOUS UREASE; UREIDE DEGRADATION; ACCESSORY PROTEIN; GENE-CLUSTER; SEED UREASE; LEAF UREASE; NICKEL; TISSUE; ENCODES; UREIDOGLYCOLATE AB The soybean genome duplicated similar to 14 and 45 million years ago and has many paralogous genes, including those in urease activation (emplacement of Ni and CO(2) in the active site). Activation requires the UreD and UreF proteins, each encoded by two paralogues. UreG, a third essential activation protein, is encoded by the single-copy Eu3, and eu3 mutants lack activity of both urease isozymes. eu2 has the same urease-negative phenotype, consistent with Eu2 being a single-copy gene, possibly encoding a Ni carrier. Unexpectedly, two eu2 alleles co-segregated with missense mutations in the chromosome 2 UreF paralogue (Ch02UreF), suggesting lack of expression/function of Ch14UreF. However, Ch02UreF and Ch14UreF transcripts accumulate at the same level. Further, it had been shown that expression of the Ch14UreF ORF complemented a fungal ureF mutant. A third, nonsense (Q2*) allelic mutant, eu2-c, exhibited 5- to 10-fold more residual urease activity than missense eu2-a or eu2-b, though eu2-c should lack all Ch02UreF protein. It is hypothesized that low-level activation by Ch14UreF is 'spoiled' by the altered missense Ch02UreF proteins ('epistatic dominant-negative'). In agreement with active 'spoiling' by eu2-b-encoded Ch02UreF (G31D), eu2-b/eu2-c heterozygotes had less than half the urease activity of eu2-c/eu2-c siblings. Ch02UreF (G31D) could spoil activation by Chr14UreF because of higher affinity for the activation complex, or because Ch02UreF (G31D) is more abundant than Ch14UreF. Here, the latter is favoured, consistent with a reported in-frame AUG in the 5' leader of Chr14UreF transcript. Translational inhibition could represent a form of 'functional divergence' of duplicated genes. C1 [Polacco, Joe C.; Medeiros-Silva, Monica] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. [Polacco, Joe C.; Medeiros-Silva, Monica] Univ Fed Rio Grande do Sul, Dept Biophys, BR-91501970 Porto Alegre, RS, Brazil. [Polacco, Joe C.; Medeiros-Silva, Monica] Univ Fed Rio Grande do Sul, Ctr Biotechnol, BR-91501970 Porto Alegre, RS, Brazil. [Hyten, David L.] ARS, Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Sleper, David A.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Sleper, David A.] Univ Missouri, Ctr Soybean Biotechnol, Columbia, MO 65211 USA. [Polacco, Joe C.; Sleper, David A.; Bilyeu, Kristin D.] Univ Missouri, Interdisciplinary Plant Grp, Bond Life Sci Ctr 371, Columbia, MO 65211 USA. [Bilyeu, Kristin D.] ARS, USDA, Plant Genet Res Unit, Columbia, MO 65211 USA. [Medeiros-Silva, Monica] Univ Fed Rio Grande do Sul, Ctr Biotechnol, Grad Program Cellular & Mol Biol, BR-91501970 Porto Alegre, RS, Brazil. RP Polacco, JC (reprint author), Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. EM polaccoj@missouri.edu OI Hyten, David/0000-0001-6324-9389 FU CAPES, Brazil FX We thank Paul Little for excellent technical assistance, Perry Cregan for encouragement in SNP mapping, Mike McMullen for providing invaluable advice on analysis of SNP data, and Doug Randall for lab space. MMS was supported by a predoctoral fellowship from CAPES, Brazil. NR 42 TC 7 Z9 7 U1 0 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD JUN PY 2011 VL 62 IS 10 BP 3599 EP 3608 DI 10.1093/jxb/err054 PG 10 WC Plant Sciences SC Plant Sciences GA 789ZA UT WOS:000292557000027 PM 21430294 ER PT J AU Cilia, M Howe, K Fish, T Smith, D Mahoney, J Tamborindeguy, C Burd, J Thannhauser, TW Gray, S AF Cilia, Michelle Howe, Kevin Fish, Tara Smith, Dawn Mahoney, Jaclyn Tamborindeguy, Cecilia Burd, John Thannhauser, Theodore W. Gray, Stewart TI Biomarker discovery from the top down: Protein biomarkers for efficient virus transmission by insects (Homoptera: Aphididae) discovered by coupling genetics and 2-D DIGE SO PROTEOMICS LA English DT Article DE Aphid; Biomarker; DIGE; Insect; Plant proteomics; Virus transmission ID TANDEM MASS-SPECTROMETRY; DNA SEQUENCE DIVERGENCE; YELLOW-DWARF VIRUSES; GEL-ELECTROPHORESIS; SCHIZAPHIS-GRAMINUM; SITOBION-AVENAE; MYZUS-PERSICAE; LUTEOVIRUS TRANSMISSION; CUTICULAR PROTEINS; HOST ASSOCIATIONS AB Yellow dwarf viruses cause the most economically important virus diseases of cereal crops worldwide and are vectored by aphids. The identification of vector proteins mediating virus transmission is critical to develop sustainable virus management practices and to understand viral strategies for circulative movement in all insect vectors. Previously, we applied 2-D DIGE to an aphid filial generation 2 population to identify proteins correlated with the transmission phenotype that were stably inherited and expressed in the absence of the virus. In the present study, we examined the expression of the DIGE candidates in previously unstudied, field-collected aphid populations. We hypothesized that the expression of proteins involved in virus transmission could be clinically validated in unrelated, virus transmission-competent, field-collected aphid populations. All putative biomarkers were expressed in the field-collected biotypes, and the expression of nine of these aligned with the virus transmission-competent phenotype. The strong conservation of the expression of the biomarkers in multiple field-collected populations facilitates new and testable hypotheses concerning the genetics and biochemistry of virus transmission. Integration of these biomarkers into current aphid-scouting methodologies will enable rational strategies for vector control aimed at judicious use and development of precision pest control methods that reduce plant virus infection. C1 [Cilia, Michelle; Howe, Kevin; Fish, Tara; Thannhauser, Theodore W.; Gray, Stewart] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Smith, Dawn; Mahoney, Jaclyn; Gray, Stewart] Cornell Univ, Dept Plant Pathol & Plant Microbe Interact, Ithaca, NY 14853 USA. [Tamborindeguy, Cecilia] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. RP Gray, S (reprint author), Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Tower Rd, Ithaca, NY 14853 USA. EM smg3@cornell.edu RI Tamborindeguy, Cecilia/A-6110-2012 FU ARS CRIS [1907-101-16, 1907-21000-24/25-00D]; NSF [DBI-0606596]; [USDA-NRI 2007-04567]; [6217-2200-015-00] FX The authors would like to thank Neil Kelleher for his encouragement and helpful discussion, the faculty and guest lecturers of the Cold Spring Harbor Laboratory 2010 Proteomics course for helpful discussions, Tom Hammond for his meticulous care of the barley and oat plants used in this study, Mike McLaughlin (USDA-ARS Ithaca) for assistance with database searching, Melissa Burrows (USDA-ARS Stillwater) for coordinating the transport of the field-collected aphid populations from Stillwater, Yong Yang for collecting LC-MS/MS data for two of the biomarkers on the SYNAPT HDMS instrument, the Cornell University Life Sciences Core Laboratories Information Technology Facility staff for assistance with database management and IT support, Kern Reeve for comments on the manuscript and helpful discussion, Kent Loeffler for help with the figures, and the following funding sources: USDA-NRI 2007-04567 (Gray and Thannhauser), ARS CRIS project numbers 1907-101-16 (Gray), 1907-21000-24/25-00D (Thannhauser), NSF DBI-0606596 (Thannhauser), and 6217-2200-015-00 (Burd). NR 80 TC 13 Z9 13 U1 0 U2 16 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1615-9853 J9 PROTEOMICS JI Proteomics PD JUN PY 2011 VL 11 IS 12 BP 2440 EP 2458 DI 10.1002/pmic.201000519 PG 19 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 793OH UT WOS:000292832500007 PM 21648087 ER PT J AU Greenberg, AS Coleman, RA AF Greenberg, Andrew S. Coleman, Rosalind A. TI Expanding roles for lipid droplets SO TRENDS IN ENDOCRINOLOGY AND METABOLISM LA English DT Editorial Material ID HORMONE-SENSITIVE LIPASE; 3T3-L1 ADIPOCYTES; STORAGE DROPLET; PERILIPIN-A; LIPOLYSIS; RESISTANCE; PROTEINS C1 [Greenberg, Andrew S.] Tufts Univ, Obes & Metab Lab, JM USDA HNRCA, Boston, MA 02111 USA. [Coleman, Rosalind A.] Univ N Carolina, Dept Nutr, Chapel Hill, NC 27599 USA. RP Greenberg, AS (reprint author), Tufts Univ, Obes & Metab Lab, JM USDA HNRCA, Boston, MA 02111 USA. EM andrew.greenberg@tufts.edu; rcoleman@unc.edu FU NIDDK NIH HHS [R01 DK56598, R01-DK59935, R01 DK082574, R01-DK-082574, R01 DK059935, R01 DK056598] NR 11 TC 12 Z9 12 U1 0 U2 2 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1043-2760 J9 TRENDS ENDOCRIN MET JI Trends Endocrinol. Metab. PD JUN PY 2011 VL 22 IS 6 SI SI BP 195 EP 196 DI 10.1016/j.tem.2011.04.002 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 791PG UT WOS:000292676500001 PM 21531573 ER PT J AU Wang, FS Tian, XL Ding, YL Wan, XC Tyree, MT AF Wang, Fusheng Tian, Xinli Ding, Yulong Wan, Xianchong Tyree, M. T. TI A survey of root pressure in 53 Asian species of bamboo SO ANNALS OF FOREST SCIENCE LA English DT Article DE Root pressure; Osmotic pressure; Asian species of bamboo ID HYDRAULIC CONDUCTIVITY RECOVERY; LAURUS-NOBILIS; XYLEM EMBOLISM; ACER-SACCHARUM; WATER; VESSELS; VINES; STEMS; MODEL; NEED AB Introduction Root pressure is a potentially important mechanism for dissolving embolisms in previously cavitated vessels, but the occurrence of root pressure is not a universal property in plants. Material and methods We suggest that root pressure might be more common in monocots than dicots because in dicots, cambial growth can replace embolized vessels but monocots have no secondary growth and hence need mechanisms for repair of embolized vessels. In this paper, bubble manometers were used to investigate 53 bamboo species from 10 genera. Results A survey of root pressure in 53 Asian species of bamboo revealed that all possessed root pressure and in some species root pressure was high enough to cause guttation of liquid from leaves at predawn. In contrast, in woody vines were we might expect root pressure to be common because of reduced cambial growth, root pressure is comparatively rare. Xylem exudate and guttation fluid had an osmotic pressure greater than root pressure and this can be explained in terms of a low reflection coefficient in context of how root pressure is generated according to the composite membrane model of roots. Conclusion We conclude that the next logical step is to survey bamboo species to determine how common cavitation evens are and to determine if refilling of vessels occurs by root pressure. C1 [Wan, Xianchong] Chinese Acad Forestry, Inst New Forest Technol, Beijing 100091, Peoples R China. [Wang, Fusheng; Tian, Xinli; Ding, Yulong] Nanjing Forestry Univ, Inst Bamboo Res, Nanjing 210037, Peoples R China. [Tyree, M. T.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Tyree, M. T.] US Forest Serv, No Res Stn, S Burlington, VT 05403 USA. RP Wan, XC (reprint author), Chinese Acad Forestry, Inst New Forest Technol, Beijing 100091, Peoples R China. EM xianchong@yahoo.com; mtyree@ales.ualberta.ca OI Ding, Yulong/0000-0002-9713-5694 FU 11th Five-year Key Technology program of China [2006BAD19B0201]; Ministry of Human Resources of China FX This work was financially supported by grants from the 11th Five-year Key Technology program of China (2006BAD19B0201) and the Ministry of Human Resources of China (2007). NR 20 TC 5 Z9 6 U1 6 U2 26 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1286-4560 J9 ANN FOREST SCI JI Ann. For. Sci. PD JUN PY 2011 VL 68 IS 4 BP 783 EP 791 DI 10.1007/s13595-011-0075-1 PG 9 WC Forestry SC Forestry GA 789XS UT WOS:000292553400012 ER PT J AU Kleinman, PJA Sharpley, AN Buda, AR McDowell, RW Allen, AL AF Kleinman, Peter J. A. Sharpley, Andrew N. Buda, Anthony R. McDowell, Richard W. Allen, Arthur L. TI Soil controls of phosphorus in runoff: Management barriers and opportunities SO CANADIAN JOURNAL OF SOIL SCIENCE LA English DT Article DE Phosphorus; runoff; eutrophication; nutrient management; no till ID WATER-QUALITY; SOUTHERN PLAINS; SURFACE WATERS; AGRICULTURAL WATERSHEDS; DISSOLVED PHOSPHORUS; SOLUBLE PHOSPHORUS; TILLAGE SYSTEMS; GRASSLAND SOILS; SATURATION; TRANSPORT AB Kleinman, P. J. A., Sharpley, A. N., Buda, A. R., McDowell, R. W. and Allen, A. L. 2011. Soil controls of phosphorus in runoff: Management barriers and opportunities. Can. J. Soil Sci. 91: 329-338. The persistent problem of eutrophication, the biological enrichment of surface waters, has produced a vast literature on soil phosphorus (P) effects on runoff water quality. This paper considers the mechanisms controlling soil P transfers from agricultural soils to runoff waters, and the management of these transfers. Historical emphases on soil conservation and control of sediment delivery to surface waters have demonstrated that comprehensive strategies to mitigate sediment-bound P transfer can produce long-term water quality improvements at a watershed scale. Less responsive are dissolved P releases from soils that have historically received P applications in excess of crop requirements. While halting further P applications to such soils may prevent dissolved P losses from growing, the desorption of P from soils that is derived from historical inputs, termed here as "legacy P", can persist for long periods of time. Articulating the role of legacy P in delaying the response of watersheds to remedial programs requires more work, delivering the difficult message that yesterday's sinks of P may be today's sources. Even legacy sources of P that occur in low concentration relative to agronomic requirement can support significant loads of P in runoff under the right hydrologic conditions. Strategies that take advantage of the capacity of soils to buffer dissolved P losses, such as periodic tillage to diminish severe vertical stratification of P in no-till soils, offer short-term solutions to mitigating P losses. In some cases, more aggressive strategies are required to mitigate both short-term and legacy P losses. C1 [Kleinman, Peter J. A.; Buda, Anthony R.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Sharpley, Andrew N.] Univ Arkansas, Dept Crop & Soil Environm Sci, Fayetteville, AR 72701 USA. [Sharpley, Andrew N.] AgResearch Ltd, Invermay Agr Ctr, Mosgiel, New Zealand. [Allen, Arthur L.] Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Princess Anne, MD 21853 USA. RP Kleinman, PJA (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM peterkleinman@ars.usda.gov RI Buda, Anthony/B-4860-2013; OI McDowell, Richard/0000-0003-3911-4825 NR 63 TC 41 Z9 41 U1 12 U2 72 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4271 J9 CAN J SOIL SCI JI Can. J. Soil Sci. PD JUN PY 2011 VL 91 IS 3 SI SI BP 329 EP 338 DI 10.4141/CJSS09106 PG 10 WC Soil Science SC Agriculture GA 788ZJ UT WOS:000292482800003 ER PT J AU Nellesen, SL Kovar, JL Haan, MM Russell, JR AF Nellesen, Shelly L. Kovar, John L. Haan, Mathew M. Russell, James R. TI Grazing management effects on stream bank erosion and phosphorus delivery to a pasture stream SO CANADIAN JOURNAL OF SOIL SCIENCE LA English DT Article DE Beef cattle; managed grazing; stream bank erosion; water quality; P ID SURFACE RUNOFF; WATER-QUALITY; CENTRAL IOWA; SEDIMENT; CATTLE; SOILS; ADJACENT; NITROGEN; USA; UK AB Nellesen, S. L., Kovar, J. L., Haan, M. M. and Russell, J. R. 2011. Grazing management effects on stream bank erosion and phosphorus delivery to a pasture stream. Can. J. Soil Sci. 91: 385-395. Pasture lands may deliver significant sediment and phosphorus (P) to surface waters. To determine the effects of beef cattle (Bos taurus) grazing practices on stream bank erosion and P losses, three treatments [rotational stocking (RS), continuous stocking with restricted stream access (CSR), and continuous stocking with unrestricted stream access (CSU)] were established in six adjacent pastures along Willow Creek in central Iowa, USA. Erosion pins were used to record bank erosion and deposition monthly from May to November of 2005-2007. Soil samples were collected by horizon to estimate P losses. Net bank erosion/deposition and pin activity, calculated as an indicator of bank stability, differed among years and measurement periods within a given year more than among grazing management treatments. Trend analysis of the 3 yr of monthly erosion/deposition data revealed two RS pastures with decreasing bank erosion, suggesting some response to this grazing practice. Mean 3-yr P losses were lower in CSR pastures (5.0 g m(-1) stream) than in RS (9.1 g m(-1) stream) and CSU (12.2 g m(-1) stream) pastures. Both sediment and P losses tended to occur during the winter and early part of the grazing season. In general, our results suggested that at this site bank erosion and P losses to the stream were controlled primarily by natural processes, rather than grazing management. C1 [Kovar, John L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Nellesen, Shelly L.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Haan, Mathew M.] Michigan State Univ, Kellogg Biol Stn, Hickory Corners, MI 49060 USA. [Russell, James R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Kovar, JL (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM john.kovar@ars.usda.gov FU Iowa Department of Natural Resources through US Environmental Protection Agency; Iowa State University Leopold Center for Sustainable Agriculture; Cooperative State Research, Education, and Extension Service, US Department of Agriculture [2006-51130-03700] FX This research was funded in part by the Iowa Department of Natural Resources through a grant from the US Environmental Protection Agency under the Federal Nonpoint Source Management Program (Section 319 of the Clean Water Act) and the Iowa State University Leopold Center for Sustainable Agriculture. The research was also supported by the Cooperative State Research, Education, and Extension Service, US Department of Agriculture, under Award No. 2006-51130-03700. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily NR 44 TC 7 Z9 7 U1 1 U2 17 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4271 J9 CAN J SOIL SCI JI Can. J. Soil Sci. PD JUN PY 2011 VL 91 IS 3 SI SI BP 385 EP 395 DI 10.4141/CJSS10006 PG 11 WC Soil Science SC Agriculture GA 788ZJ UT WOS:000292482800008 ER PT J AU Armstrong, SD Smith, DR Owens, PR Joern, BC Leytem, AB Huang, CH Adeola, O AF Armstrong, Shalamar D. Smith, Douglas R. Owens, Phillip R. Joern, Brad C. Leytem, April B. Huang, Chi H. Adeola, Olayiwola TI Phosphorus and nitrogen loading depths in fluvial sediments following manure spill simulations SO CANADIAN JOURNAL OF SOIL SCIENCE LA English DT Article DE Manure spills; sediment; nitrogen; phosphorus; mixing depth ID MIDWESTERN UNITED-STATES; DRAINAGE DITCHES; STREAM; FATE; ADSORPTION; MEHLICH-3; EMISSIONS; IMPACTS; AMMONIA; CYCLE AB Armstrong, S. D., Smith, D. R., Owens, P. R., Joern, B. C., Leytem, A. B., Huang, C. H. and Adeola, O. 2011. Phosphorus and nitrogen loading depths in fluvial sediments following manure spill simulations. Can. J. Soil Sci. 91: 427-436. The depth of nitrogen (N) and phosphorus (P) loading in fluvial sediments following a manure spill has not been documented. Thus, the objectives of this study were: (i) to determine the depth of N and P enrichment as a result of a manure spill under base flow conditions using fluvarium techniques and (ii) to evaluate the impact of sediment particle size distribution on N and P enrichment depths. Manure spills were simulated using stream simulators and ditch sediments collected from agricultural drainage ditches. During the manure spill simulation, the P sorption capacity of all sediments exponentially decreased with time and the NH(4)-N sorption capacity remained constant with time. The P and NH(4)-N loading in all sediments were observed to the depth of 2 cm, but were most concentrated in the 0- to 1-cm depth ranging in concentrations from 3 to 12 mg P kg(-1) and from 7.2 to 45 mg NH(4)-N kg(-1). Data from this study give a basis for the advancement of manure spill remediation that will reduce the impairment of surface waters via the release of nutrients from enriched sediments following a manure spill. C1 [Armstrong, Shalamar D.; Smith, Douglas R.; Huang, Chi H.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Owens, Phillip R.; Joern, Brad C.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Adeola, Olayiwola] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. RP Armstrong, SD (reprint author), USDA ARS, Natl Soil Eros Res Lab, 275 S Russell St, W Lafayette, IN 47907 USA. EM Sarmstro@purdue.edu FU Illinois-Indiana Sea Grant College; Alliance for Graduate Education and Professoriate FX The authors are grateful to Jeremy Shulman, Scott McAfee, Amanda McKenzie, Myriam Larose, and Taneisha Springfield-Jones for their assistance in completing this research. Additionally, the authors acknowledge the Illinois-Indiana Sea Grant College Program and the Alliance for Graduate Education and Professoriate for funding that supported this research. NR 38 TC 3 Z9 3 U1 1 U2 8 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4271 J9 CAN J SOIL SCI JI Can. J. Soil Sci. PD JUN PY 2011 VL 91 IS 3 SI SI BP 427 EP 436 DI 10.4141/CJSS10003 PG 10 WC Soil Science SC Agriculture GA 788ZJ UT WOS:000292482800011 ER PT J AU Jokela, WE Casler, MD AF Jokela, William E. Casler, Michael D. TI Transport of phosphorus and nitrogen in surface runoff in a corn silage system: Paired watershed methodology and calibration period results SO CANADIAN JOURNAL OF SOIL SCIENCE LA English DT Article DE Phosphorus; nitrogen; runoff; paired watershed; water quality; corn silage ID MANURE APPLICATION; AGRICULTURAL PHOSPHORUS; SEDIMENT LOSSES; RESIDUE LEVEL; COVER CROPS; TILLAGE; SOIL; NUTRIENT; SNOWMELT; QUALITY AB Joke la, W. E and Casler, M. D. 2011. Transport of phosphorus and nitrogen in surface runoff in a corn silage system: Paired watershed methodology and calibration period results. Can. J. Soil Sci. 91: 479-491. Transport of P, N, and sediment via runoff from crop fields, especially where manure has been applied, can contribute to eutrophication and degradation of surface waters. We established a paired-watershed field site to evaluate surface runoff losses of nutrients and sediment from different manure/crop/tillage management systems for silage corn production. During the 2-yr calibration period the four 1.6-ha watersheds, or fields, were treated identically with fall dairy manure application and chisel plowing, and runoff was monitored, sampled, and analyzed for suspended sediment (SS) and total and dissolved forms of P and N. That management was maintained as a control in one watershed, while alternative management systems were initiated on the three treatment fields. During the calibration period both concentrations and loads of SS and total and dissolved P and N varied by field and over 50% of runoff and dissolved P and N was from snowmelt runoff. Linear regressions of treatment fields against the control field were highly significant for runoff and concentrations and loads of all constituents. The estimated minimum detectable change (difference between means) was 10 to 30% for most parameters, suggesting a reasonable probability of success in detecting change in the treatment period. C1 [Jokela, William E.] USDA ARS, Marshfield, WI USA. [Casler, Michael D.] US Dairy Forage Res Ctr, Madison, WI USA. RP Jokela, WE (reprint author), USDA ARS, Marshfield, WI USA. EM bill.jokela@ars.usda.gov NR 35 TC 8 Z9 8 U1 4 U2 21 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4271 J9 CAN J SOIL SCI JI Can. J. Soil Sci. PD JUN PY 2011 VL 91 IS 3 SI SI BP 479 EP 491 DI 10.4141/CJSS09095 PG 13 WC Soil Science SC Agriculture GA 788ZJ UT WOS:000292482800016 ER PT J AU Makarova, SS Erokhina, TN Solovyev, AG Schiemann, J Owens, R Morozov, SY AF Makarova, S. S. Erokhina, T. N. Solovyev, A. G. Schiemann, J. Owens, R. Morozov, S. Y. TI Subcellular localization and structural properties of tobacco 4/1 protein SO FEBS JOURNAL LA English DT Meeting Abstract CT 36th FEBS Congress of the Biochemistry for Tomorrows Medicine CY JUN 25-30, 2011 CL Torino, ITALY SP Federat Soc Biochem & Mol Biol C1 [Makarova, S. S.] Moscow MV Lomonosov State Univ, Dept Virol, Moscow, Russia. [Erokhina, T. N.] Shemyakin & Ovchinnikov Inst Bioorgan Chem, Dept Mol Diagnost, Moscow, Russia. [Solovyev, A. G.; Morozov, S. Y.] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow, Russia. [Schiemann, J.] JKI, Inst Biosafety Genetically Modified Plants, Quedlinburg, Germany. [Owens, R.] USDA ARS, Beltsville Agr Res Ctr, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1742-464X J9 FEBS J JI FEBS J. PD JUN PY 2011 VL 278 SU 1 SI SI BP 465 EP 465 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 786UJ UT WOS:000292333103064 ER PT J AU Kendra, PE Sanchez, JS Montgomery, WS Okins, KE Niogret, J Pena, JE Epsky, ND Heath, RR AF Kendra, Paul E. Sanchez, Jorge S. Montgomery, Wayne S. Okins, Katherine E. Niogret, Jerome Pena, Jorge E. Epsky, Nancy D. Heath, Robert R. TI DIVERSITY OF SCOLYTINAE (COLEOPTERA: CURCULIONIDAE) ATTRACTED TO AVOCADO, LYCHEE, AND ESSENTIAL OIL LURES SO FLORIDA ENTOMOLOGIST LA English DT Article DE ambrosia beetles; Persea americana; Litchi chinensis; manuka oil; phoebe oil ID AMBROSIA BEETLE COLEOPTERA; SOUTHEASTERN UNITED-STATES; LAUREL WILT; LAURACEAE; REDBAY; SYMBIONT; PATHOGEN; VECTOR AB The redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), is an exotic wood-boring insect that vectors laurel wilt, a lethal vascular disease of trees in the Lauraceae, including avocado (Persea americana) and native Persea species (redbay, swampbay). As part of research to identify host-based attractants for X. glabratus, we discovered that a diverse array of non-target ambrosia beetles was attracted to the same substrates as X. glabratus. During Sep-Dec 2009, several field tests were conducted in north Florida (in woodlands with advanced stages of laurel wilt) with traps baited with commercial lures of the essential oils, manuka and phoebe, and with freshly-cut wood bolts of avocado (a known host) and lychee (Litchi chinensis, a non-host high in the sesquiterpene alpha-copaene, a putative host attractant). In addition, manuka-baited traps were deployed in avocado groves in south Florida to monitor for potential spread of X. glabratus. The combined trapping results indicated that none of these substrates was specific in attraction of X. glabratus. Numerous non-target ambrosia beetles were captured, including 17 species representative of 4 tribes within the subfamily Scolytinae. This report provides photo-documentation and data on the species diversity and relative abundance for a group of poorly-studied beetles, the scolytine community in Florida Persea habitats. C1 [Kendra, Paul E.; Sanchez, Jorge S.; Montgomery, Wayne S.; Niogret, Jerome; Epsky, Nancy D.; Heath, Robert R.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Okins, Katherine E.] Florida Dept Agr & Consumer Serv, DPI, CAPS, Gainesville, FL 32608 USA. [Pena, Jorge E.] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; Florida Avocado Administrative Committee FX We gratefully acknowledge David Long, Mike Winterstein (USDA-ARS; Miami, FL), Rita Duncan (Univ. Florida; Homestead, FL), Gurpreet Brar, and Stephen McLean (Univ. Florida; Gainesville, FL) for technical assistance; Patti Anderson (FDACS-DPI, Gainesville, FL) for Persea identifications; Bud Mayfield (USDA-Forest Service; Asheville, NC) for advice on field trapping; Ray Schnell (USDA-ARS; Miami, FL) for advice on avocado germplasm samples; David Jenkins (USDA-ARS; Mayaguez, PR) and 2 anonymous reviewers for suggestions with the manuscript; Pansy Vazquez-Kendra and Elena Schnell for translation of the abstract; and Connie Rightmire (St. John's River Water Management District) for assistance in obtaining a special use permit for the Lochloosa Wildlife Conservation Area. This work was supported in part by the USDA-ARS National Plant Disease Recovery System and the Florida Avocado Administrative Committee. This report presents the results of research only; mention of a proprietary product does not constitute an endorsement by the USDA. NR 18 TC 16 Z9 17 U1 3 U2 17 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 123 EP 130 PG 8 WC Entomology SC Entomology GA 787WH UT WOS:000292406900001 ER PT J AU Pereira, R Sivinski, J Shapiro, JP Teal, PEA AF Pereira, Rui Sivinski, John Shapiro, Jeffrey P. Teal, Peter E. A. TI INFLUENCE OF METHOPRENE AND DIETARY PROTEIN ON MALE ANASTREPHA SUSPENSA (DIPTERA: TEPHRITIDAE) LIPID AND PROTEIN CONTENT SO FLORIDA ENTOMOLOGIST LA English DT Article DE adult age; adult weight; Caribbean fruit fly; hydrolyzed yeast; juvenile hormone; sexual maturation ID FRUIT-FLIES DIPTERA; MATING SUCCESS; LOEW DIPTERA; ADULT DIET; SEXUAL SUCCESS; FLY DIPTERA; FOOD; RESERVES; SIZE; CONSUMPTION AB Because both the application of a juvenile hormone analog, methoprene, and the addition of protein to the adult diet increased the sexual success of male Caribbean fruit fly, Anastrepha suspensa (Loew) (Diptera: Tephritidae), it was hypothesized that both might also impact male nutritional status. Total content of lipid and of protein in A. suspensa males were measured to discover if there was an effect of these treatments alone or in combination on the content of each of these subtstances. In the first 24 hours following adult emergence, 6 different treatments were applied (all possible combinations of methoprene in acetone solution or acetone alone, and protein-diet enrichment). Adult weight was determined for all treatments at 5, 10, 15, 20, 25, 30 and 35 d post-emergence. Dietary protein had a positive effect on the weight and total lipid and protein contents during the first 35 d of adult male life. There were minimal negative impacts from methoprene applications. Even though males were more active sexually, there was no significant change in weight or protein content during the study period. However, total lipid content decreased with age. The usefulness of methoprene to enhance the sexual performance of mass-reared tephritids destined for sterile release appears to outweigh any physiological costs/limitations that such treatment might confer. C1 [Pereira, Rui] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Pereira, Rui; Sivinski, John; Shapiro, Jeffrey P.; Teal, Peter E. A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. RP Pereira, R (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. FU International Atomic Energy Agency [12863]; Centro de Ciencia e Tecnologia da Madeira [BD I/2002-004] FX We thank David Nestel (IPP-The Volcani Center, Beit-Dagan, Israel), Nikos Papadopoulos (University of Thessaly, Magnisia, Greece), and Steve Ferkovich (CMAVE, USDA-ARS, Gainesville-FL, USA) for critical reviews of an earlier version of this manuscript. This project was funded in part by the International Atomic Energy Agency (Research Contract 12863). Financial support was provided to RP by the Centro de Ciencia e Tecnologia da Madeira through the Ph.D. grant BD I/2002-004. NR 44 TC 2 Z9 2 U1 1 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 137 EP 144 PG 8 WC Entomology SC Entomology GA 787WH UT WOS:000292406900003 ER PT J AU Buckingham, GR Wineriter, SA Stanley, JD Pratt, PD Center, TD AF Buckingham, Gary R. Wineriter, Susan A. Stanley, Jason D. Pratt, Paul D. Center, Ted D. TI VAGILITY AS A LIABILITY: RISK ASSESSMENT OF THE LEAF-BLOTCHING BUG EUCEROCORIS SUSPECTUS (HEMIPTERA: MIRIDAE), A PROSPECTIVE BIOLOGICAL CONTROL AGENT OF THE AUSTRALIAN TREE MELALEUCA QUINQUENERVIA SO FLORIDA ENTOMOLOGIST LA English DT Article DE Biological control; Hemiptera; Eucerocoris suspectus; host range; Melaleuca quinquenervia; Miridae; Myrtaceae; risk assessment; weed control ID HOST-RANGE; MYRTACEAE AB Melaleuca quinqueaervia (Cav.) S.T. Blake (Myrtales: Myrtaceae) forms dense monocultures that displace native vegetation in wetlands of southern Florida, USA. Faunal studies in the tree's native Australian range revealed several prospective biological control agents, including the leaf-blotching bug, Eucerocoris suspectus Distant (Hemiptera: Miridae). This herbivore was imported into quarantine to assess risk to Florida native and ornamental species after preliminary Australian studies had indicated that it might be useful. Ornamental Melaleuca spp. suffered heavy feeding in no-choice adult feeding trials, with moderate feeding on some native Myrtaceae. Native species sustained light to heavy feeding in multi-choice adult feeding trials and in a no-choice nymphal feeding trial. Feeding increased on native species in a large enclosure after M. quinquenervia was cut, allowed to dry, and then removed. Nymphs completed development only on M. quinquenervia and ornamental bottlebrushes, Melaleuca spp. However, inability to fully develop on non-target species is of limited importance as a criterion for release of insects with highly mobile immature stages as compared to less vagile species. Local movement from the host to other plant species could result in unacceptable non-target damage despite seemingly adequate developmental specificity. This insect would clearly harm native and ornamental Myrtaceae and should therefore not be released. C1 [Buckingham, Gary R.; Wineriter, Susan A.] ARS, USDA, Invas Plants Res Lab, Gainesville, FL 32614 USA. [Stanley, Jason D.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA. [Pratt, Paul D.; Center, Ted D.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. FU South Florida Water Management District; Florida Department of Environmental Protection Bureau of Invasive Plant Management FX We thank Mayana Roberg Anderson for assistance with plant maintenance. We further acknowledge D. W. Burrows, J. K. Balciunas, M. F. Purcell, K. E. Galway, J. A. Goolsby, J. R. Makinson, D. Mira, and the late C. E. Turner for significant contributions towards the study of Eucerocoris suspectus. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This research was supported, in part, by grants from the South Florida Water Management District and the Florida Department of Environmental Protection Bureau of Invasive Plant Management. NR 8 TC 1 Z9 1 U1 0 U2 6 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 172 EP 179 PG 8 WC Entomology SC Entomology GA 787WH UT WOS:000292406900008 ER PT J AU Epsky, ND Kendra, PE Pena, J Heath, RR AF Epsky, Nancy D. Kendra, Paul E. Pena, Jorge Heath, Robert R. TI COMPARISON OF SYNTHETIC FOOD-BASED LURES AND LIQUID PROTEIN BAITS FOR CAPTURE OF ANASTREPHA SUSPENSA (DIPTERA: TEPHRITIDAE) ADULTS SO FLORIDA ENTOMOLOGIST LA English DT Article DE Caribbean fruit fly; Biolure; ammonium acetate; unipak; torula yeast ID FRUIT-FLIES DIPTERA; FLY DIPTERA; CERATITIS-CAPITATA; YEAST HYDROLYSATE; ATTRACTANT; TRAP; FORMULATIONS; BIOLURE; LUDENS; CITRUS AB Field tests were conducted in south Florida to compare capture of the Caribbean fruit fly, Anastrepha suspensa (Loew), in Multilure traps baited with either of the liquid protein baits torula yeast/borax or Nulure/borax, or with food-based synthetic lures including two-component Biolure (ammonium acetate, putrescine) and three-component Biolure (ammonium acetate, putrescine, trimethylamine). The highest relative proportion of females captured was in traps baited with the two-component Biolure (44-61%), intermediate capture was in traps baited with the three-component Biolure (14-24%) or torula yeast/borax (8-25%), and the lowest capture tended to be in traps baited with Nulure/borax (0-19%). Similar results were obtained for capture of males. Tests of the unipak two-component Biolure, which has a reduced ammonium acetate release rate and is a single package with both ammonium acetate and putrescine sections, captured similar numbers of both females and males as Biolure formulated in 2 individual packages. Traps baited with unipak Biolure combined with the addition of a trimethylamine lure captured fewer females than the unipak alone, but this was greater than capture in traps baited with torula yeast/borax. Our studies confirmed that the best lure for A. suspensa is ammonium acetate and putrescine. However, C. capitata-targeted traps baited with three-component Biolure should be as effective for A. suspensa detection and monitoring as traps baited with torula yeast/borax. The unipak two-component Biolure will provide the improved handling that has been requested by users. C1 [Epsky, Nancy D.; Kendra, Paul E.; Heath, Robert R.] ARS, Subtrop Hort Res Stn, USDA, Miami, FL 33158 USA. [Pena, Jorge] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. RP Epsky, ND (reprint author), ARS, Subtrop Hort Res Stn, USDA, 13601 Old Cutler Rd, Miami, FL 33158 USA. NR 21 TC 6 Z9 10 U1 1 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 180 EP 185 PG 6 WC Entomology SC Entomology GA 787WH UT WOS:000292406900009 ER PT J AU Jenkins, DA Epsky, ND Kendra, PE Heath, RR Goenaga, R AF Jenkins, David A. Epsky, Nancy D. Kendra, Paul E. Heath, Robert R. Goenaga, Ricardo TI FOOD-BASED LURE PERFORMANCE IN THREE LOCATIONS IN PUERTO RICO: ATTRACTIVENESS TO ANASTREPHA SUSPENSA AND A. OBLIQUA (DIPTERA: TEPHRITIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE ammonium acetate; putrescine; Nulure; McPhail trap ID FRUIT-FLIES DIPTERA; SYNTHETIC ATTRACTANT; MCPHAIL TRAPS; LOEW DIPTERA; FLY DIPTERA; AMMONIA; FORMULATIONS; PUTRESCINE; EFFICACY; BIOLURE AB Lures based on odors released by hydrolyzed protein were assessed for their attractiveness to Anastrepha obliqua and A. suspensa at 3 locations in Puerto Rico in Aug through Oct 2009. Lures compared included ammonium acetate combined with putrescine, hydrolyzed corn protein (Nulure) with borax, freeze-dried Nulure, freeze-dried Nulure in combination with ammonium acetate, freeze-dried Nulure in combination with ammonium acetate and putrescine, and the Unipak lure, a single lure containing ammonium acetate and putrescine. Where the distribution of trapped flies departed significantly from what would be expected given an equal attraction of the baits, Nulure and freeze-dried Nulure always attracted fewer flies than the other baits tested, regardless of species, sex, or location. Although all of the baits or bait combinations containing ammonium acetate attracted more flies than the Nulure or freeze-dried Nulure baits, there was a distinct trend of ammonium acetate and putrescine and the Unipak lures to attract more flies after the 4th week of the study and for the freeze-dried Nulure with ammonium acetate or in combination with ammonium acetate and putrescine to attract more flies in the 1st 4 weeks of the study. This trial is unique in that it was conducted in orchards of carambola, Averrrhoa carambola (Oxalidaceae), a poor host for both fly species. Our results are compared with other studies on lures of A. obliqua and A. suspensa and the implications for monitoring/detecting pest Tephritidae are discussed. C1 [Jenkins, David A.; Goenaga, Ricardo] ARS, USDA, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Epsky, Nancy D.; Kendra, Paul E.; Heath, Robert R.] ARS, USDA, Subtrop Hort Res Stn, Miami, FL 33158 USA. RP Jenkins, DA (reprint author), ARS, USDA, Trop Agr Res Stn, 2200 Ave PA Campos, Mayaguez, PR 00680 USA. NR 29 TC 4 Z9 5 U1 0 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 186 EP 194 PG 9 WC Entomology SC Entomology GA 787WH UT WOS:000292406900010 ER PT J AU Porter, SD Graham, LC Johnson, SJ Thead, LG Briano, JA AF Porter, Sanford D. Graham, L. C. Fudd Johnson, Seth J. Thead, Larry G. Briano, Juan A. TI THE LARGE DECAPITATING FLY PSEUDACTEON LITORALIS (DIPTERA: PHORIDAE): SUCCESSFULLY ESTABLISHED ON FIRE ANT POPULATIONS IN ALABAMA SO FLORIDA ENTOMOLOGIST LA English DT Article DE Solenopsis invicta; biological control; low population density ID SOLENOPSIS-SAEVISSIMA COMPLEX; FLIES DIPTERA; SOUTH-AMERICA; HYMENOPTERA-FORMICIDAE; CURVATUS DIPTERA; NORTH FLORIDA; PARASITOIDS; TRICUSPIS; ARGENTINA; PATTERNS AB The large fire ant decapitating fly, Pseudacteon litoralis Borgmeier, from northeastern Argentina was successfully released as a self-sustaining biocontrol agent of imported fire ants in south central Alabama in 2005. Five years later, this fly is firmly established at the original release site and has expanded outward at least 18 km. Nevertheless, populations remain very low considering P litoralis is one of the most abundant fire ant decapitating flies in large areas of its range in South America. The reasons for low densities and why we were only able to establish this fly at 1 of 9 release sites in 4 states (2003-2006) are unknown, but problems with host-matching, release procedures, weather conditions, and competition with previously released decapitating flies are discussed as possible factors. C1 [Porter, Sanford D.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Graham, L. C. Fudd] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Johnson, Seth J.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Thead, Larry G.] ARS, Biol Control Pests Res Unit, USDA, Stoneville, MS 38776 USA. [Briano, Juan A.] ARS, S Amer Biol Control Lab, USDA, Buenos Aires, DF, Argentina. RP Porter, SD (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 25 TC 8 Z9 9 U1 0 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 208 EP 213 PG 6 WC Entomology SC Entomology GA 787WH UT WOS:000292406900013 ER PT J AU Tufts, DM Spencer, K Hunter, WB Bextine, B AF Tufts, Danielle M. Spencer, Kyle Hunter, Wayne B. Bextine, Blake TI DELIVERY SYSTEM USING SODIUM ALGINATE VIRUS LOADED PELLETS TO RED IMPORTED FIRE ANTS (SOLENOPSIS INVICTA, HYMENOPTERA: FORMICIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE ant management; anti-infecting virus; Solenopsis invicta virus (SINV); microencapsulation; mortality; biological control ID GLASSY-WINGED SHARPSHOOTER; COLONY COLLAPSE DISORDER; GENOME SEQUENCE; DISCOVERY; SINV-1 AB Microencapsulation as a delivery mechanism of SINV-1 and other molecules such as dsRNA, offers an approach to Solenopsis invicta Buren management that is target specific and fits current approaches to baiting ants with toxins and/or RNA-interference. The delivery method presented here targets ground dwelling, foraging ants with an ant-infecting virus which is specific to the genus, Solenopsis. Endemic ant-infecting viruses, like S. invicta viruses (SINV-1, SINV-2, and SINV-3) are being evaluated for efficacy in S. invicta population suppression. In this study, SINV-1 (TX5 strain) was extracted from S. invicta colonies and microencapsulated in sodium alginate pellets. Pellets containing extracted whole virions were offered to confirmed non-infected S. invicta colonies. Colonies were sampled every 5 d and tested by reverse transcription polymerase chain reaction (RT-PCR) for presence of viral RNA. The longevity of control and viral pellets were also evaluated. Within 30 d, post-feeding of virus, 35% of S. invicta colonies acquired SINV-1 infection (P = 0.03). Thus, microencapsulation as a delivery mechanism was successful to deliver SINV-1 to S. inuicta colonies. Future incorporation of this economically affordable method can be implemented to deliver biological agents for specific ant species and to augment current approaches that bait ants. While a virus was used to demonstrate delivery, an adequate and affordable virus production system still needs to be developed before a viral strategy can be adopted as a tool for biological control of fire ants. C1 [Tufts, Danielle M.; Spencer, Kyle; Bextine, Blake] Univ Texas Tyler, Dept Biol, Tyler, TX 75799 USA. [Hunter, Wayne B.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Tufts, DM (reprint author), Univ Nebraska, Sch Biol Sci, 323 Manter Hall, Lincoln, NE 68588 USA. FU University of Texas FX We thank our anonymous reviews for constructive criticism and Christopher Powell for assistance in the laboratory. Funding was provided by a University of Texas at Tyler research grant. The mention or use of products within does not imply nor guarantee an endorsement by the USDA, AIRS, to the exclusion of other similar, suitable products. NR 16 TC 4 Z9 4 U1 1 U2 11 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 237 EP 241 PG 5 WC Entomology SC Entomology GA 787WH UT WOS:000292406900017 ER PT J AU Armstrong, JS Adamczyk, JJ Greenberg, SM AF Armstrong, J. Scott Adamczyk, John J., Jr. Greenberg, Shoil M. TI EFFICACY OF SINGLE AND DUAL GENE COTTON GOSSYPIUM HIRSUTUM EVENTS ON NEONATE AND THIRD INSTAR FALL ARMYWORM, SPODOPTERA FRUGIPERDA DEVELOPMENT BASED ON TISSUE AND MERIDIC DIET ASSAYS SO FLORIDA ENTOMOLOGIST LA English DT Article DE Bacillus thuringiensis; Cry1Ac; Cry2Ab; Cry1F+Cry1Ac; GMO; PIP; transgenic ID INSECTICIDAL PROTEINS; LEPIDOPTERA-NOCTUIDAE; EXPRESSING ONE; LARVAE; SURVIVAL; BERLINER AB We evaluated mortality and developmental parameters of fall armyworms, Spodoptera frugiperda (J. E. Smith), to the single Bacillus thuringiensis (Bt) cotton trait, Bollgard (R) and dual Bt cotton traits (Bollgard II (R) and WideStrike (TM)) by using a cotton leaf-tissue assay and by incorporating lyophilized cotton tissue into a meridic diet. Bioassays were conducted for both neonate and 3rd instars. Leaf tissue bioassays indicated that Bollgard II (R) and WideStrike (TM) are highly effective against fall armyworm neonates by causing mortality and by retarding development parameters such as larval weight, pupal duration, and time to adulthood. Bollgard (R) was not significantly different from non-transgenic cotton in terms of mortality or feeding, with the exception of the non-Bt (PhytoGen 425RF), which had an inherent form of resistance that is not associated with a transgenic event. Third instars evaluated with lyophilized diet bioassays were not as affected by the Bt traits to the same degree as neonates; however, larval weights were lower, and developmental parameters such as time to pupation and time to adulthood were longer. The duration of pupal development was significantly longer for 3rd instars that survived the highest dose of 5,000 mu g of WideStrike (TM) cotton tissue. Sublethal doses for Bollgard II (R) and WideStrike (TM) were generally observed at 500 to 5,000 mu g of lyophilized cotton tissue per mg of meridic diet, depending upon the variable (time to pupation, pupal duration, time to adult emergence) measured. C1 [Armstrong, J. Scott; Adamczyk, John J., Jr.; Greenberg, Shoil M.] ARS, USDA, Beneficial Insect Res Unit, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Armstrong, JS (reprint author), ARS, USDA, Beneficial Insect Res Unit, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. NR 18 TC 3 Z9 4 U1 0 U2 9 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 262 EP 271 PG 10 WC Entomology SC Entomology GA 787WH UT WOS:000292406900020 ER PT J AU Hardke, JT Temple, JH Leonard, BR Jackson, RE AF Hardke, Jarrod T. Temple, Joshua H. Leonard, B. Rogers Jackson, Ryan E. TI LABORATORY TOXICITY AND FIELD EFFICACY OF SELECTED INSECTICIDES AGAINST FALL ARMYWORM (LEPIDOPTERA: NOCTUIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE Spodoptera frugiperda; dose-mortality responses; chemical control; IPM ID COTTON; LARVAE; SORGHUM AB Fall armyworm, Spodoptera frugiperda (J. E. Smith), is an occasional but often serious pest of several row crops in the southern U.S., including cotton, field corn, and grain sorghum. The objective of these studies was to generate baseline dose-mortality responses for fall armyworm larvae in laboratory bioassays, to confirm field efficacy against natural infestations, and to determine residual efficacy of selected insecticides. These studies evaluated 4 recently developed insecticides (chlorantraniliprole, cyantraniliprole, flubendiamide, and spinetoram) and 5 commercial standards (indoxacarb, lambda-cyhalothrin, methoxyfenozide, novaluron, and spinosad). In diet-incorporated assays, the LC(50) values of chlorant-ranilprole and spinetoram were significantly lower than the LC(50)'s of all other insecticides. The results of a field trial against a native fall armyworm infestation in grain sorghum indicated that chlorantraniliprole reduced the number of infested whorls below that in the non-treated control and the lambda-cyhalothrin- and methoxyfenozide-treated plots at 3 d after treatment (DAT). At 7 DAT, no insecticides significantly reduced the number of infested whorls below that in the non-treated plots. In residual efficacy studies, exposure of fall armyworm larvae to chlorantraniliprole- and cyantraniliprole-treated tissue resulted in significantly greater mortality compared to those exposed to non-treated tissue and lambda-cyhalothrin-, flubendiamide-, novaluron-, and methoxyfenozide-treated tissues at 7 DAT. In addition, chlorantraniliprole and cyantraniliprole were the only compounds that resulted in >40% mortality at 28 DAT. These results indicate that newer insecticides are equal to or more efficacious against fall armyworm than traditional insecticides. C1 [Hardke, Jarrod T.; Temple, Joshua H.; Leonard, B. Rogers] Louisiana State Univ, LSU AgCtr, Baton Rouge, LA 70803 USA. [Jackson, Ryan E.] ARS, USDA, So Insect Management Res Unit, Stoneville, MS USA. RP Hardke, JT (reprint author), Louisiana State Univ, LSU AgCtr, Baton Rouge, LA 70803 USA. FU LSU AgCenter; Cotton Incorporated; Louisiana Soybean and Grain Promotion Board FX The authors thank Trey Price, Ralph Sheppard, Karla Emfinger, and the numerous student workers at the Macon Ridge Research Station for assistance with laboratory and field studies, and colony maintenance. The authors thank the LSU AgCenter, Cotton Incorporated, and the Louisiana Soybean and Grain Promotion Board for financial support. This article was approved for publication by the Director of the Louisiana Agricultural Experiment Station as manuscript No. 2010-258-9502. NR 25 TC 19 Z9 21 U1 2 U2 19 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 272 EP 278 PG 7 WC Entomology SC Entomology GA 787WH UT WOS:000292406900021 ER PT J AU Sanders, WR Mankin, RW Liburd, OE Stelinski, LL AF Sanders, William R. Mankin, R. W. Liburd, Oscar E. Stelinski, Lukasz L. TI ACOUSTIC DETECTION OF ARTHROPOD INFESTATION OF GRAPE ROOTS: SCOUTING FOR GRAPE ROOT BORER (LEPIDOPTERA: SESIIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE IPM; monitoring; mounding; grape pest; Vitis spp. ID SEX-PHEROMONE; SOIL AB The grape root borer, Vitacea polistiformis Harris, is the principal pest of grapes (Vitis spp. L.) in Florida where chlorpyrifos is 1 of the few chemicals registered for its control. However, chlorpyrifos is not an ideal treatment because it is highly toxic to birds, fish, aquatic invertebrates, and honeybees. Also, the recommended timing of application conflicts with harvest dates. There is an effective cultural control method, known as mounding, but this method is currently cost prohibitive for commercial production and is not widely used. If mounding could be applied only to infested plants, the cost of this method would be reduced considerably. This study evaluated the potential of acoustics for detecting the larvae in-situ. Human listeners assessed likelihood of arthropod infestation for each site based on live acoustic samples as they were being recorded. Computer software later constructed acoustic indicators from these recordings that were used for computer assessment of infestation likelihood. After recording, the roots of sampled vines were excavated to determine infestation levels. Infestation likelihood predictions of both human listeners and computer software largely reflected infestation condition of tested sites. Consequently, acoustic methods could be developed as tools for growers to employ mounding only at sites most likely to be infested, and thus enable more cost-effective use of this cultural control tactic. C1 [Sanders, William R.; Liburd, Oscar E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Stelinski, Lukasz L.] Univ Florida, Ctr Citrus Res & Educ, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. RP Sanders, WR (reprint author), Univ Florida, Dept Entomol & Nematol, 970 Nat Area Dr, Gainesville, FL 32611 USA. FU Florida Grape Growers Association; SARE [G000340] FX We thank the staff in the Small Fruit and Vegetable IPM laboratory at the University of Florida, and Everett Foreman, Betty Weaver, and Mackenzie Egan at USDA-ARS-CMAVE in Gainesville for assistance in field and laboratory work. We would also like to thank Lyle Buss at the University of Florida for his assistance with specimen identification. We also thank the grape growers that participated in the study, Bob Paulish and John Sirvent, without whom this research would not have been possible. Partial funding was provided by the Florida Grape Growers Association and SARE grant #G000340. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 17 TC 1 Z9 1 U1 2 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 296 EP 302 PG 7 WC Entomology SC Entomology GA 787WH UT WOS:000292406900024 ER PT J AU Ammar, E Hall, DG AF Ammar, El-Desouky Hall, David G. TI A NEW METHOD FOR SHORT-TERM REARING OF CITRUS PSYLLIDS (HEMIPTERA: PYSLLIDAE) AND FOR COLLECTING THEIR HONEYDEW EXCRETIONS SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material C1 [Ammar, El-Desouky; Hall, David G.] ARS, Subtrop Insects Res Unit, USDA, Ft Pierce, FL 34945 USA. RP Ammar, E (reprint author), ARS, Subtrop Insects Res Unit, USDA, Ft Pierce, FL 34945 USA. NR 6 TC 5 Z9 5 U1 1 U2 15 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 340 EP 342 PG 3 WC Entomology SC Entomology GA 787WH UT WOS:000292406900029 ER PT J AU Hesler, LS Lago, PK AF Hesler, Louis S. Lago, Paul K. TI FIRST RECORDS OF BOTH SUBSPECIES OF BRACHIACANTHA QUADRIPUNCTATA (COLEOPTERA: COCCINELLIDAE) IN MISSISSIPPI, USA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID NORTH; AMERICA; DAKOTA; LIST C1 [Hesler, Louis S.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Lago, Paul K.] Univ Mississippi, Dept Biol, University, MS 38677 USA. RP Hesler, LS (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2011 VL 94 IS 2 BP 361 EP 363 PG 3 WC Entomology SC Entomology GA 787WH UT WOS:000292406900038 ER PT J AU Zhu, HP Altland, J Derksen, RC Krause, CR AF Zhu, Heping Altland, James Derksen, Richard C. Krause, Charles R. TI Optimal Spray Application Rates for Ornamental Nursery Liner Production SO HORTTECHNOLOGY LA English DT Article DE bareroot stock; environment; nursery sprayer; pest management; spray coverage; spray deposition ID OFF-TARGET LOSS; DROPLET DENSITY; LARVAE AB Spray deposition and coverage at different application rates for nursery liners of different sizes were investigated to determine the optimal spray application rates. Experiments were conducted on 2- and 3-year-old 'Autumn Spire' red maple (Acer rubrum) liners. A traditional hydraulic sprayer with vertical booms between tree rows was used to apply the spray applications. Application rates were 10, 20, 30, and 40 gal/acre for the 2-year-old liners and were 20, 40, 60, and 80 gal/acre for the 3-year-old liners. Nylon screens were used to collect spray deposition of a fluorescent tracer dissolved in water, and water-sensitive papers were used to quantify spray coverage inside canopies. Spray deposition, coverage, and droplet density inside both 2- and 3-year-old liner canopies increased as the application rate increased. The minimum rates to spray 6.6-ft-tall, 2-year-old 'Autumn Spire' red maple liners and 8.7-ft-tall, 3-year-old liners were 20 and 40 gal/acre, respectively. An exponential equation was derived from these results to estimate the spray application rate required for different tree liner heights and to minimize excessive chemical use in rapidly growing tree liners. C1 [Zhu, Heping; Altland, James; Derksen, Richard C.; Krause, Charles R.] ARS, USDA, Applicat Technol Res Unit, Wooster, OH 44691 USA. RP Zhu, HP (reprint author), ARS, USDA, Applicat Technol Res Unit, 1680 Madison Ave, Wooster, OH 44691 USA. EM heping.zhu@ars.usda.gov NR 16 TC 6 Z9 7 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2011 VL 21 IS 3 BP 367 EP 375 PG 9 WC Horticulture SC Agriculture GA 787WS UT WOS:000292408000015 ER PT J AU Yan, F Cao, HW Cover, TL Washington, MK Shi, Y Liu, LS Chaturvedi, R Peek, RM Wilson, KT Polk, DB AF Yan, Fang Cao, Hanwei Cover, Timothy L. Washington, M. Kay Shi, Yan Liu, LinShu Chaturvedi, Rupesh Peek, Richard M., Jr. Wilson, Keith T. Polk, D. Brent TI Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID GROWTH-FACTOR RECEPTOR; ACTIVE ULCERATIVE-COLITIS; PLACEBO-CONTROLLED TRIAL; SULFATE-INDUCED COLITIS; LACTOBACILLUS-GG; EPITHELIAL-CELLS; CROHNS-DISEASE; OXAZOLONE COLITIS; INDUCED APOPTOSIS; IMMUNE-RESPONSES AB Probiotic bacteria can potentially have beneficial effects on the clinical course of several intestinal disorders, but our understanding of probiotic action is limited. We have identified a probiotic bacteria-derived soluble protein, p40, from Lactobacillus rbamnosus GG (LGG), which prevents cytokine-induced apoptosis in intestinal epithelial cells. In the current study, we analyzed the mechanisms by which p40 regulates cellular responses in intestinal epithelial cells and p40's effects on experimental colitis using mouse models. We show that the recombinant p40 protein activated EGFR, leading to Akt activation. Activation of EGFR by p40 was required for inhibition of cytokine-induced apoptosis in intestinal epithelial cells in vitro and ex vivo. Furthermore, we developed a pectin/zein hydrogel bead system to specifically deliver p40 to the mouse colon, which activated EGFR in colon epithelial cells. Administration of p40-containing beads reduced intestinal epithelial apoptosis and disruption of barrier function in the colon epithelium in an EGFR-dependent manner, thereby preventing and treating DSS-induced intestinal injury and acute colitis. Furthermore, p40 activation of EGFR was required for ameliorating colon epithelial cell apoptosis and chronic inflammation in oxazolone-induced colitis. These data define what we believe to be a previously unrecognized mechanism of probiotic-derived soluble proteins in protecting the intestine from injury and inflammation. C1 [Yan, Fang; Cao, Hanwei; Polk, D. Brent] Monroe Carell Jr Childrens Hosp Vanderbilt, Dept Pediat, Div Gastroenterol Hepatol & Nutr, Nashville, TN USA. [Cover, Timothy L.; Chaturvedi, Rupesh; Peek, Richard M., Jr.; Wilson, Keith T.] Vanderbilt Univ, Sch Med, Dept Med, Nashville, TN 37232 USA. [Cover, Timothy L.] Vanderbilt Univ, Sch Med, Dept Microbiol & Immunol, Nashville, TN 37232 USA. [Cover, Timothy L.; Chaturvedi, Rupesh; Wilson, Keith T.] Vet Affairs Tennessee Valley Healthcare Syst, Nashville, TN USA. [Washington, M. Kay] Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA. [Shi, Yan] Xiamen Univ, Sch Life Sci, Xiamen, Peoples R China. [Liu, LinShu] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Peek, Richard M., Jr.; Wilson, Keith T.] Vanderbilt Univ, Sch Med, Dept Canc Biol, Nashville, TN 37232 USA. [Polk, D. Brent] Vanderbilt Univ, Sch Med, Dept Cell & Dev Biol, Nashville, TN 37232 USA. RP Yan, F (reprint author), Vanderbilt Univ, Med Ctr, Dept Pediat, Div Gastroenterol Hepatol & Nutr, 2215 Garland Ave,MRB 4,Room 1035, Nashville, TN 37232 USA. EM fang.yan@vanderbilt.edu RI Cover, Timothy/I-3814-2015; OI Cover, Timothy/0000-0001-8503-002X; Wilson, Keith/0000-0003-4421-1830 FU NIH [DK065744, DK081134]; Crohn's and Colitis Foundation of America [DK56008, AT004821, AT004821-S1, DK053620]; Department of Veterans Affairs; Vanderbilt University Digestive Disease Research Center [P30DK058404]; Vanderbilt University Medical Center Imaging Core Research Laboratory [CA 68485] FX This work was supported by NIH grants DK065744 and DK081134 and a Crohn's and Colitis Foundation of America Senior Research Award (to F. Yan); DK56008 (to D.B. Polk); AT004821, AT004821-S1, and DK053620 (to K.T. Wilson); the Department of Veterans Affairs (to T.L. Cover and K.T. Wilson); P30DK058404 (Vanderbilt University Digestive Disease Research Center); and CA 68485 (Vanderbilt University Medical Center Imaging Core Research Laboratory). NR 56 TC 109 Z9 115 U1 2 U2 45 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JUN PY 2011 VL 121 IS 6 BP 2242 EP 2253 DI 10.1172/JCI44031 PG 12 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 772LB UT WOS:000291234300024 PM 21606592 ER PT J AU Finkenstadt, VL Cote, GL Willett, JL AF Finkenstadt, Victoria L. Cote, Gregory L. Willett, J. L. TI Corrosion protection of low-carbon steel using exopolysaccharide coatings from Leuconostoc mesenteroides SO BIOTECHNOLOGY LETTERS LA English DT Article DE Corrosion; Exopolysaccharide; Leuconostoc mesenteroides ID STRUCTURAL-ANALYSIS; NRRL B-1355; BIOFILM; GLUCANSUCRASE; METHYLATION; INHIBITION AB Corrosion of metals is a serious and challenging problem faced worldwide by industry. Purified Leuconostoc mesenteroides exopolysaccharide (EPS) coatings, cast from aqueous solution, inhibited the corrosion of low-carbon steel as determined by electrochemical impedance spectroscopy (EIS). There were two different corrosion behaviors exhibited when EPS films from different strains were cast onto the steel. One EPS coating reacted immediately with the steel substrate to form an iron (III) oxide layer ("rust") during the drying process while another did not. The samples that did not flash corrode had higher corrosion inhibition and formed an iron (II) passivation layer during EIS testing that persisted after the cells were disassembled. Corrosion inhibition was strain-specific as polysaccharides with similar structure did not have the same corrosion potential. C1 [Finkenstadt, Victoria L.; Cote, Gregory L.; Willett, J. L.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Finkenstadt, VL (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM victoria.finkenstadt@ars.usda.gov NR 24 TC 19 Z9 20 U1 1 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD JUN PY 2011 VL 33 IS 6 BP 1093 EP 1100 DI 10.1007/s10529-011-0539-2 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 777WG UT WOS:000291655200003 PM 21290167 ER PT J AU Manitchotpisit, P Price, NPJ Leathers, TD Punnapayak, H AF Manitchotpisit, Pennapa Price, Neil P. J. Leathers, Timothy D. Punnapayak, Hunsa TI Heavy oils produced by Aureobasidium pullulans SO BIOTECHNOLOGY LETTERS LA English DT Article DE Aureobasidium pullulans; Fluorescence; Heavy oil ID 5-HYDROXY-2-DECENOYL ESTERS; POLYOL LIPIDS; 3,5-DIHYDROXYDECANOYL; ARABITOL; MANNITOL; STRAINS AB From a survey of more than 50 diverse strains of Aureobasidium pullulans, 21 produced extracellular heavy oils. Most oil producers fell into phylogenetic clades 8, 9, and 11. Oil colors ranged from bright yellow to malachite. More than half of the strains produced oil that was fluorescent. In medium containing 5% (w/v) sucrose, oil yields ranged from 0.5 to 6 g oil/l. Strain CU 43 reached stationary growth phase at day 4 while oil yields were maximal at day 6. CU 43 produced bright yellow, highly fluorescent oil that also was visible as intracellular droplets under fluorescent microscopy. Oil was surface active, suggesting that it functions as a biosurfactant. Oil from two strains (CU 43 and NRRL Y-12974) differentially inhibited mammalian cancer cell lines. MALDI-TOF MS spectra suggested that A. pullulans strains produce a family of related oil structures. C1 [Price, Neil P. J.; Leathers, Timothy D.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Rangsit Univ, Biochem Unit, Dept Med Sci, Fac Sci, Lakhok 12000, Pathumthani, Thailand. [Punnapayak, Hunsa] Chulalongkorn Univ, Plant Biomass Utilizat Res Unit, Dept Bot, Fac Sci, Bangkok 10330, Thailand. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Tim.Leathers@ars.usda.gov FU Research Center of Rangsit University [37/52] FX The authors would like to acknowledge RSU grant number 37/52 from the Research Center of Rangsit University for financial support. The authors sincerely thank Dr. Christopher Dunlap for surface tension measurements. Appreciation is expressed for the kind assistance provided by Melinda Nunnally, Joseph Rich, Karen Hughes, Nathane Orwig, and Trina Hartman. NR 12 TC 18 Z9 18 U1 2 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD JUN PY 2011 VL 33 IS 6 BP 1151 EP 1157 DI 10.1007/s10529-011-0548-1 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 777WG UT WOS:000291655200012 PM 21293903 ER PT J AU Oakley, BB Talundzic, E Morales, CA Hiett, KL Siragusa, GR Volozhantsev, NV Seal, BS AF Oakley, Brian B. Talundzic, Eldin Morales, Cesar A. Hiett, Kelli L. Siragusa, Gregory R. Volozhantsev, Nikolay V. Seal, Bruce S. TI Comparative genomics of four closely related Clostridium perfringens bacteriophages reveals variable evolution among core genes with therapeutic potential SO BMC GENOMICS LA English DT Article ID TETRANUCLEOTIDE USAGE PATTERNS; MICROBIAL COMMUNITIES; PROTEIN FAMILIES; GROWTH PROMOTERS; POULTRY; DOMAINS; VISUALIZATION; ANNOTATION; SEQUENCES; BINDING AB Background: Because biotechnological uses of bacteriophage gene products as alternatives to conventional antibiotics will require a thorough understanding of their genomic context, we sequenced and analyzed the genomes of four closely related phages isolated from Clostridium perfringens, an important agricultural and human pathogen. Results: Phage whole-genome tetra-nucleotide signatures and proteomic tree topologies correlated closely with host phylogeny. Comparisons of our phage genomes to 26 others revealed three shared COGs; of particular interest within this core genome was an endolysin (PF01520, an N-acetylmuramoyl-L-alanine amidase) and a holin (PF04531). Comparative analyses of the evolutionary history and genomic context of these common phage proteins revealed two important results: 1) strongly significant host-specific sequence variation within the endolysin, and 2) a protein domain architecture apparently unique to our phage genomes in which the endolysin is located upstream of its associated holin. Endolysin sequences from our phages were one of two very distinct genotypes distinguished by variability within the putative enzymatically-active domain. The shared or core genome was comprised of genes with multiple sequence types belonging to five pfam families, and genes belonging to 12 pfam families, including the holin genes, which were nearly identical. Conclusions: Significant genomic diversity exists even among closely-related bacteriophages. Holins and endolysins represent conserved functions across divergent phage genomes and, as we demonstrate here, endolysins can have significant variability and host-specificity even among closely-related genomes. Endolysins in our phage genomes may be subject to different selective pressures than the rest of the genome. These findings may have important implications for potential biotechnological applications of phage gene products. C1 [Oakley, Brian B.; Morales, Cesar A.; Hiett, Kelli L.; Siragusa, Gregory R.; Seal, Bruce S.] ARS, Poultry Microbiol Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. [Talundzic, Eldin] Univ Georgia, Dept Infect Dis, Athens, GA 30306 USA. [Talundzic, Eldin] Univ Georgia, Ctr Trop & Emerging Global Dis, Athens, GA 30306 USA. [Volozhantsev, Nikolay V.] State Res Ctr Appl Microbiol & Biotechnol, Obolensk, Russia. [Siragusa, Gregory R.] Danisco Inc, Waukesha, WI 53186 USA. RP Oakley, BB (reprint author), ARS, Poultry Microbiol Res Unit, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM brian.oakley@ars.usda.gov OI Volozhantsev, Nikolay/0000-0003-4667-1451 FU ARS-USDA [6612-32000-046, 6612-32000-055] FX This work was supported by ARS-USDA project number 6612-32000-046 Interventions and Methodologies to Reduce Human Food-Borne Bacterial Pathogens in Chickens and project number 6612-32000-055 Molecular Characterization and Gastrointestinal Tract Ecology of Commensal Human Food-Borne Bacterial Pathogens in the Chicken. We thank Johnna Garrish for technical assistance and Susan Brooks for assistance with manuscript preparation. NR 48 TC 12 Z9 12 U1 2 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 1 PY 2011 VL 12 AR 282 DI 10.1186/1471-2164-12-282 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 778UC UT WOS:000291731300001 PM 21631945 ER PT J AU Scholljegerdes, EJ Lekatz, LA Vonnahme, KA AF Scholljegerdes, E. J. Lekatz, L. A. Vonnahme, K. A. TI Effects of short-term oilseed supplementation on reproductive performance in beef heifers SO CANADIAN JOURNAL OF ANIMAL SCIENCE LA English DT Article DE Flaxseed; lipid supplementation; omega-3 fatty acids; omega-6 fatty acids; pregnancy rate ID LINOLEATE SAFFLOWER SEEDS; FATTY-ACID-COMPOSITION; BODY CONDITION SCORE; DAIRY-COWS; ESTROUS BEHAVIOR; HOLSTEIN COWS; BRAHMAN COWS; DIETARY-FAT; PROGESTERONE; PROSTAGLANDIN AB Scholljegerdes, E. J., Lekatz, L. A. and Vonnahme, K. A. 2011. Effects of short-term oilseed supplementation on reproductive performance in beef heifers. Can. J. Anim. Sci. 91: 221-229. The objective of this experiment was to evaluate the role of a short-term increase in linolenic acid around the timed artificial insemination (TAI) on plasma fatty acid concentrations, estrous behavior, and pregnancy. Therefore, 96 cross-bred heifers (Initial BW = 394 +/- 18.1 kg) fed chopped grass hay were allotted to one of three dietary treatments: no supplement (CON); 1.8 kg head(-1) d(-1) (DM basis) of a soybean-based pellet (SOY) high in linoleic acid (C18:2n-6); or 1.5 kg head(-1) d(-1) (DM basis) of a flaxseed-based pellet (FLX) high in linolenic acid (C18:3n-3). On day -9, relative to TAI, supplementation began, and heifers received a controlled internal drug-releasing (CIDR) device and gonadotropin releasing hormone (GnRH; 100 mu g). On day -2, CIDRs were removed and heifers received 25 mg prostaglandin F(2)alpha (PGF(2)alpha). Heifers were bred by TAI (day 0) 54 h after CIDR removal and received GnRH. Estrous behavior was monitored throughout the feeding period by radiotelemetry. Supplementation ended on day 18 relative to TAI. Plasma concentrations of C18:2n-6 were greater by day 0 and day 18 (P < 0.001) above that of CON for both SOY and FLX with SOY being greater than (P < 0.001) FLX. Likewise, C18:3n-3 was also greater for supplemented heifers by day 0 and day 18 (P < 0.001). The number of heifers that did not respond to estrous synchronization was not affected by dietary treatment (P=0.61). Conception to AI. was not affected by treatment (P=0.24). Overall pregnancy rates tended to be lower for FLX (P=0.08) than SOY. Conception to a TAI program was not enhanced when oilseeds were fed to beef heifers during estrous synchronization and continuing past day 18 relative to artificial insemination. C1 [Scholljegerdes, E. J.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Lekatz, L. A.; Vonnahme, K. A.] N Dakota State Univ, Ctr Nutr & Pregnancy, Dept Anim Sci, Fargo, ND 58105 USA. RP Scholljegerdes, EJ (reprint author), New Mexico State Univ, Las Cruces, NM 88003 USA. EM ejs@nmsu.edu NR 49 TC 1 Z9 1 U1 1 U2 4 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-3984 J9 CAN J ANIM SCI JI Can. J. Anim. Sci. PD JUN PY 2011 VL 91 IS 2 BP 221 EP 229 DI 10.4141/CJAS2010-045 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 779LX UT WOS:000291780200004 ER PT J AU Robertson, GP Hamilton, SK Del Grosso, SJ Parton, WJ AF Robertson, G. Philip Hamilton, Stephen K. Del Grosso, Stephen J. Parton, William J. TI The biogeochemistry of bioenergy landscapes: carbon, nitrogen, and water considerations SO ECOLOGICAL APPLICATIONS LA English DT Article DE bioenergy; biofuels; cellulosic biomass; climate mitigation; climate stabilization; ecosystem modeling; global warming potential; greenhouse gases; nitrogen; nitrous oxide; soil carbon; water quality ID LIFE-CYCLE ASSESSMENT; LAND-USE CHANGE; CROPPING SYSTEMS; UNITED-STATES; ETHANOL-PRODUCTION; MISSISSIPPI RIVER; GREENHOUSE GASES; OXIDE EMISSIONS; CORN STOVER; BIOFUELS AB The biogeochemical liabilities of grain-based crop production for bioenergy are no different from those of grain-based food production: excessive nitrate leakage, soil carbon and phosphorus loss, nitrous oxide production, and attenuated methane uptake. Contingent problems are well known, increasingly well documented, and recalcitrant: freshwater and coastal marine eutrophication, groundwater pollution, soil organic matter loss, and a warming atmosphere. The conversion of marginal lands not now farmed to annual grain production, including the repatriation of Conservation Reserve Program (CRP) and other conservation set-aside lands, will further exacerbate the biogeochemical imbalance of these landscapes, as could pressure to further simplify crop rotations. The expected emergence of biorefinery and combustion facilities that accept cellulosic materials offers an alternative outcome: agricultural landscapes that accumulate soil carbon, that conserve nitrogen and phosphorus, and that emit relatively small amounts of nitrous oxide to the atmosphere. Fields in these landscapes are planted to perennial crops that require less fertilizer, that retain sediments and nutrients that could otherwise be transported to groundwater and streams, and that accumulate carbon in both soil organic matter and roots. If mixed-species assemblages, they additionally provide biodiversity services. Biogeochemical responses of these systems fall chiefly into two areas: carbon neutrality and water and nutrient conservation. Fluxes must be measured and understood in proposed cropping systems sufficient to inform models that will predict biogeochemical behavior at field, landscape, and regional scales. Because tradeoffs are inherent to these systems, a systems approach is imperative, and because potential biofuel cropping systems and their environmental contexts are complex and cannot be exhaustively tested, modeling will be instructive. Modeling alternative biofuel cropping systems converted from different starting points, for example, suggests that converting CRP to corn ethanol production under conventional tillage results in substantially increased net greenhouse gas (GHG) emissions that can be only partly mitigated with no-till management. Alternatively, conversion of existing cropland or prairie to switchgrass production results in a net GHG sink. Outcomes and policy must be informed by science that adequately quantifies the true biogeochemical costs and advantages of alternative systems. C1 [Robertson, G. Philip; Hamilton, Stephen K.] Michigan State Univ, WK Kellogg Biol Stn, Hickory Corners, MI 49060 USA. [Robertson, G. Philip; Hamilton, Stephen K.] Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [Robertson, G. Philip] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA. [Hamilton, Stephen K.] Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA. [Del Grosso, Stephen J.; Parton, William J.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80521 USA. [Del Grosso, Stephen J.] ARS, USDA, Ft Collins, CO 80512 USA. RP Robertson, GP (reprint author), Michigan State Univ, WK Kellogg Biol Stn, Hickory Corners, MI 49060 USA. EM robertson@kbs.msu.edu RI Hamilton, Stephen/N-2979-2014; Robertson, G/H-3885-2011 OI Hamilton, Stephen/0000-0002-4702-9017; Robertson, G/0000-0001-9771-9895 FU DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science and DOE OBP EERE); NSF; Michigan Agricultural Experiment Station FX Support for this work was provided by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science and DOE OBP EERE), the NSF Long-term Ecological Research Program, and the Michigan Agricultural Experiment Station. NR 81 TC 50 Z9 50 U1 11 U2 144 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2011 VL 21 IS 4 BP 1055 EP 1067 DI 10.1890/09-0456.1 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 776LE UT WOS:000291535500007 PM 21774413 ER PT J AU Hoover, CM Heath, LS AF Hoover, Coeli M. Heath, Linda S. TI Potential gains in C storage on productive forestlands in the northeastern United States through stocking management SO ECOLOGICAL APPLICATIONS LA English DT Article DE forest carbon management; forest carbon sequestration; Forest Inventory and Analysis; Forest Vegetation Simulator; northeastern United States forests AB One method of increasing forest carbon stocks that is often discussed is increasing stocking levels on existing forested lands. However, estimates of the potential increases in forest carbon sequestration as a result of increased stocking levels are not readily available. Using the USDA Forest Service's Forest Inventory and Analysis data coupled with the Forest Vegetation Simulator, we estimate that, for a seven-state region in the northeastern United States, timberland contains about 1768 Tg of carbon in aboveground live biomass across all stocking classes. If all medium and understocked stands had the carbon density of fully stocked stands, an additional 453 Tg of carbon would be stored. While the carbon gains per unit area are greatest for understocked stands, generally fewer than 10% of stands are in this condition. The increase in carbon storage per unit area is smaller for stands in the medium stocked class, but the large proportion of stands in this condition offers considerable opportunities. Our analysis indicates that, when seeking to increase forest carbon storage, managing stocking levels is an option with considerable potential, especially since no changes in land use are required. C1 [Hoover, Coeli M.; Heath, Linda S.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Hoover, CM (reprint author), US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. EM choover@fs.fed.us NR 17 TC 8 Z9 9 U1 0 U2 10 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2011 VL 21 IS 4 BP 1154 EP 1161 DI 10.1890/10-0046.1 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 776LE UT WOS:000291535500014 PM 21774420 ER PT J AU Nave, LE Vance, ED Swanston, CW Curtis, PS AF Nave, Lucas E. Vance, Eric D. Swanston, Christopher W. Curtis, Peter S. TI Fire effects on temperate forest soil C and N storage SO ECOLOGICAL APPLICATIONS LA English DT Article DE carbon sinks; fire; forest management; meta-analysis; soil carbon; soil nitrogen; temperate forests ID PONDEROSA PINE FOREST; COARSE WOODY DEBRIS; ORGANIC-MATTER; TERRESTRIAL ECOSYSTEMS; NORTHERN WISCONSIN; STAND REPLACEMENT; SIERRA-NEVADA; CARBON SINKS; WILDFIRE; NITROGEN AB Temperate forest soils store globally significant amounts of carbon (C) and nitrogen (N). Understanding how soil pools of these two elements change in response to disturbance and management is critical to maintaining ecosystem services such as forest productivity, greenhouse gas mitigation, and water resource protection. Fire is one of the principal disturbances acting on forest soil C and N storage and is also the subject of enormous management efforts. In the present article, we use meta-analysis to quantify fire effects on temperate forest soil C and N storage. Across a combined total of 468 soil C and N response ratios from 57 publications (concentrations and pool sizes), fire had significant overall effects on soil C (-26%) and soil N (-22%). The impacts of fire on forest floors were significantly different from its effects on mineral soils. Fires reduced forest floor C and N storage (pool sizes only) by an average of 59% and 50%, respectively, but the concentrations of these two elements did not change. Prescribed fires caused smaller reductions in forest floor C and N storage (-46% and -35%) than wildfires (-67% and -69%), and the presence of hardwoods also mitigated fire impacts. Burned forest floors recovered their C and N pools in an average of 128 and 103 years, respectively. Among mineral soils, there were no significant changes in C or N storage, but C and N concentrations declined significantly (-11% and -12%, respectively). Mineral soil C and N concentrations were significantly affected by fire type, with no change following prescribed burns, but significant reductions in response to wildfires. Geographic variation in fire effects on mineral soil C and N storage underscores the need for region-specific fire management plans, and the role of fire type in mediating C and N shifts (especially in the forest floor) indicates that averting wildfires through prescribed burning is desirable from a soils perspective. C1 [Nave, Lucas E.; Curtis, Peter S.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Nave, Lucas E.] Univ Michigan, Biol Stn, Pellston, MI 49769 USA. [Vance, Eric D.] Natl Council Air & Stream Improvement, Res Triangle Pk, NC 27709 USA. [Swanston, Christopher W.] US Forest Serv, USDA, No Res Stn, Houghton, MI 49931 USA. RP Nave, LE (reprint author), Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. EM lukenave@umich.edu FU USDA-Forest Service Northern Research Station [06-JV-11242300]; National Soil Carbon Network FX This research was supported by the USDA-Forest Service Northern Research Station through Cooperative Agreement No. 06-JV-11242300. The National Soil Carbon Network also supported this work. We acknowledge John Clark, Jim Le Moine, and Robert Sanford for helpful conversations during the preparation of the manuscript, and Alex Friend, who helped define the scope of our larger meta-analysis project at its initiation. NR 78 TC 43 Z9 45 U1 5 U2 76 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2011 VL 21 IS 4 BP 1189 EP 1201 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 776LE UT WOS:000291535500017 PM 21774423 ER PT J AU Shuster, WD Pappas, E AF Shuster, W. D. Pappas, E. TI Laboratory Simulation of Urban Runoff and Estimation of Runoff Hydrographs with Experimental Curve Numbers Implemented in USEPA SWMM SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING-ASCE LA English DT Article DE Storm water management; Rainfall intensity; Hydrologic models; Infiltration; Urban areas; Runoff; Hydrographs ID SYSTEMS AB The prognostic capabilities of a lumped hydrologic modeling approach may be complicated by routing and connectivity among infiltrative and impervious surfaces. We used artificial rainfall to generate runoff from impervious and bare soil boxes arranged in series to simulate different extents and connectivity of impervious surfaces under different moisture conditions for pervious areas. Curve numbers were calculated from observed rainfall and runoff data, compared with published values, and used in the curve number infiltration algorithm in the U.S. EPA Storm Water Management Model 5 (USEPA SWMM5) to generate runoff hydrographs. Experimental curve numbers were higher than tabular USDA values, ranging from 91 to 96. Simulations of infiltration and runoff response with experimental curve numbers showed overall good agreement with observed data, although SWMM5 was unable to re-create early term infiltration patterns, and simulated runoff lagged observed, which is attributed to implicit accounting for soil moisture and other assumptions of the SWMM5 curve number application. Our results highlight some prospects for the use of curve numbers in modeling infiltration and runoff.DOI:10.1061/(ASCE)IR.1943-4774.0000301. (C) 2011 American Society of Civil Engineers. C1 [Shuster, W. D.] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Sustainable Environm Branch, Cincinnati, OH 45268 USA. [Pappas, E.] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Shuster, WD (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, Sustainable Environm Branch, ML498,26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM shuster.william@epa.gov NR 15 TC 5 Z9 5 U1 6 U2 45 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 J9 J IRRIG DRAIN E-ASCE JI J. Irrig. Drainage Eng-ASCE PD JUN PY 2011 VL 137 IS 6 BP 343 EP 351 DI 10.1061/(ASCE)IR.1943-4774.0000301 PG 9 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA 777PF UT WOS:000291634600001 ER PT J AU Chumlea, WMC Cesari, M Evans, WJ Ferrucci, L Fielding, RA Pahor, M Studenski, S Vellas, B AF Chumlea, W. M. C. Cesari, M. Evans, W. J. Ferrucci, L. Fielding, R. A. Pahor, M. Studenski, S. Vellas, B. CA Task Force Members TI Sarcopenia: Designing Phase IIB Trials International working group on Sarcopenia SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Article DE Sarcopenia; phase IIB clinical trials; fat-free mass; strength; frailty ID RANDOMIZED CONTROLLED-TRIAL; PERIPHERAL ARTERIAL-DISEASE; SKELETAL-MUSCLE MASS; HORMONE AND/OR TESTOSTERONE; LIFE-STYLE INTERVENTIONS; HEALTHY OLDER-ADULTS; FRAIL ELDERLY-MEN; BODY-COMPOSITION; PHYSICAL-ACTIVITY; GROWTH-HORMONE AB Sarcopenia is the age-related involuntary loss of skeletal muscle mass and functionality that can lead to the development of disability, frailty and increased health care costs. The development of interventions aimed at preventing and/or treating sarcopenia is complex, requiring the adoption of assumptions and standards that are not well established scientifically or clinically. A number of investigators and clinicians (both from academia and industry) met in Rome (Italy) in 2009 to develop a consensus definition of sarcopenia. Subsequently, in Albuquerque (New Mexico, USA) in 2010, the same group met again to consider the complex issues necessary for designing Phase II clinical trials for sarcopenia. Current clinical trial data indicate that fat-free mass (FFM) parameters are responsive to physical activity/nutritional treatment modalities over short time periods, but pharmacological trials of sarcopenia have yet to show significant efficacy. In order to conduct a clinical trial within a reasonable time frame, groups that model or display accelerated aging and loss of FFM are necessary. Few studies have used acceptable designs for testing treatment effects, sample sizes or primary outcomes that could provide interpretable findings or effects across studies. Dual energy x-ray absorptiometry (DXA) is the measure of choice for assessing FFM, but sufficient time is needed for changes to be detected accurately and reliably. A tool set that would allow clinical, basic and epidemiological research on sarcopenia to advance rapidly toward diagnosis and treatment phases should be those reflecting function and strength. C1 [Chumlea, W. M. C.] Wright State Univ, Boonshoft Sch Med, Dept Community Hlth, Lifespan Hlth Res Ctr, Dayton, OH 45420 USA. [Chumlea, W. M. C.; Cesari, M.; Vellas, B.] Univ Toulouse 3, Inserm U 1027, CHU Purpan, F-31300 Toulouse, France. [Evans, W. J.] GlaxoSmithKline Inc, Head Muscle Metab DPU, Res Triangle Pk, NC 27709 USA. [Ferrucci, L.] Harbor Hosp, Clin Res Branch, Baltimore, MD 21225 USA. [Fielding, R. A.] Tufts Univ, Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Pahor, M.] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL 32610 USA. [Studenski, S.] Univ Pittsburgh, Pittsburgh Vet Affairs Med Ctr, Pittsburgh, PA USA. RP Chumlea, WMC (reprint author), Wright State Univ, Boonshoft Sch Med, Dept Community Hlth, Lifespan Hlth Res Ctr, 3171 Res Blvd, Dayton, OH 45420 USA. EM cameron.chumlea@wright.edu; william.j.evans@gsk.com; ferruccilu@grc.nia.nih.gov; MPahor@aging.ufl.edu; sas33@pitt.edu RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 FU USDA [58-1950-7-707]; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679] FX This material is based upon work supported by the USDA, under agreement No. 58-1950-7-707 Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U. S. Department of Agriculture. Also supported by the Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679). NR 80 TC 32 Z9 32 U1 1 U2 6 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD JUN PY 2011 VL 15 IS 6 BP 450 EP 455 DI 10.1007/s12603-011-0092-7 PG 6 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA 777VM UT WOS:000291653200005 PM 21623466 ER PT J AU Meydani, M Das, S Band, M Epstein, S Roberts, S AF Meydani, M. Das, S. Band, M. Epstein, S. Roberts, S. TI The effect of caloric restriction and glycemic load on measures of oxidative stress and antioxidants in humans: Results from the calerie trial of human caloric restriction SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Article DE Calorie restriction; antioxidants; oxidative stress; GPX; protein carbonyl; humans ID RANDOMIZED CONTROLLED-TRIAL; LIFE-SPAN REGULATION; DIETARY RESTRICTION; PROTEIN OXIDATION; WEIGHT-LOSS; LIPID-PEROXIDATION; ENZYME-ACTIVITY; OXIDANT STRESS; IN-VIVO; AGE AB Decreasing oxidative stress and increasing antioxidant defense has been hypothesized as one mechanism by which caloric restriction (CR) increases longevity in animals. A total of 46 moderately overweight volunteers (BMI: 252-30 kg/m(2)), ages 20-42 yr were randomized to either high glycemic (HG) or low glycemic (LG) dietary load CR regimen at either 10% (n=12) or 30% (n=34) of basal caloric intake. All food was provided to participants for 6 mo. Overall, after controlling for CR levels and dietary regimen for 6 mo, plasma glutathione peroxidase activity increased (p=0.04) and plasma protein carbonyl levels decreased (p=0.02) and a non-significant decrease in plasma 8-epi-prostaglandin F2 alpha level was observed (p=0.09). No significant change was observed in other plasma antioxidants such as superoxide dismutase and catalase. These findings indicate that short term CR (10% or 30%) in moderately overweight subjects modulates some but not all measures of antioxidant defense and oxidative stress. C1 [Meydani, M.; Das, S.; Band, M.; Epstein, S.; Roberts, S.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. RP Meydani, M (reprint author), Tufts Univ, Vasc Biol Lab, JM USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM mohsen.meydani@tufts.edu FU NIA/NIH [NGA-3U01-AG20480]; U.S. Department of Agriculture [58-1950-7-707] FX This manuscript is based on work supported by grant from NIA/NIH grant NGA-3U01-AG20480 and the U.S. Department of Agriculture, under agreement No. 58-1950-7-707. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. We would also like to thank Stephanie Marco for her assistance in the preparation of this manuscript. NR 41 TC 14 Z9 14 U1 0 U2 19 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD JUN PY 2011 VL 15 IS 6 BP 456 EP 460 DI 10.1007/s12603-011-0002-z PG 5 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA 777VM UT WOS:000291653200006 PM 21623467 ER PT J AU Lopes, AP Santos, H Neto, F Rodrigues, M Kwok, OCH Dubey, JP Cardoso, L AF Lopes, A. P. Santos, H. Neto, F. Rodrigues, M. Kwok, O. C. H. Dubey, J. P. Cardoso, L. TI PREVALENCE OF ANTIBODIES TO TOXOPLASMA GONDII IN DOGS FROM NORTHEASTERN PORTUGAL SO JOURNAL OF PARASITOLOGY LA English DT Article ID TISSUE CYSTS; INFECTION; OOCYSTS; CATS; SPECIFICITY; SENSITIVITY; PORK AB Prevalence of antibodies to Toxoplasma gondii was investigated in 673 domestic dogs from northeastern Portugal, using the modified agglutination test (MAT) with 1:20 as cutoff for seropositivity; antibodies were found in 256 dogs (38.0%). Differences between seroprevalence levels in males (36.7%) and females (41.8%) and between pure-breed (42.1%) and mixed-breed dogs (35.2%) were not statistically significant. Multiple logistic regression analysis identified age above 12 mo (odds ratio [OR] = 4.0), chance of eating birds or small mammals (OR = 4.0), housing exclusively outdoors (OR = 1.5), home-cooked meals (OR = 3.0), and eating raw meat or viscera (OR = 7.7) as risk factors for the canine T. gondii infection. Some control measures are suggested based on these findings. C1 [Lopes, A. P.; Santos, H.; Neto, F.; Rodrigues, M.; Kwok, O. C. H.; Dubey, J. P.; Cardoso, L.] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, P-5001801 Vila Real, Portugal. RP Dubey, JP (reprint author), ARS, Anim Parasit Dis Lab, Anim Nat Resources Inst, USDA, Beltsville, MD 20705 USA. EM jitender.dubey@anri.barc.usda.gov RI Cardoso, Luis/J-8310-2013; Lopes, Ana Patricia/K-4671-2013; OI Cardoso, Luis/0000-0002-6145-7560; Lopes, Ana Patricia/0000-0002-8182-5674 FU Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/44438/2008]; European Social Fund FX The authors thank the dog owners for their cooperation. Special thanks are also due to F. T. Rodrigues, D. Diz-Lopes, T. Coutinho, C. Abraao, D. Jesus, and F. Fraga for their assistance. A. P. Lopes was supported by scholarship SFRH/BD/44438/2008 from Fundacao para a Ciencia e a Tecnologia, Portugal, and the European Social Fund. NR 18 TC 17 Z9 17 U1 0 U2 6 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2011 VL 97 IS 3 BP 418 EP 420 DI 10.1645/GE-2691.1 PG 3 WC Parasitology SC Parasitology GA 779KA UT WOS:000291775300007 PM 21506866 ER PT J AU Dangoudoubiyam, S Oliveira, JB Viquez, C Gomez-Garcia, A Gonzalez, O Romero, JJ Kwok, OCH Dubey, JP Howe, DK AF Dangoudoubiyam, S. Oliveira, J. B. Viquez, C. Gomez-Garcia, A. Gonzalez, O. Romero, J. J. Kwok, O. C. H. Dubey, J. P. Howe, D. K. TI Detection of Antibodies Against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in Horses From Costa Rica SO JOURNAL OF PARASITOLOGY LA English DT Article ID SEROLOGIC PREVALENCE; SERUM ANTIBODIES; SURFACE-ANTIGEN; NORTH-AMERICA; CANINUM; BRAZIL; HUGHESI; ASSAY AB Serum samples from 315 horses from Costa Rica, Central America, were examined for the presence of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii by using the surface antigen (SAG) SnSAG2 enzyme-linked immunosorbent assay (ELISA), the NhSAG1 ELISA, and the modified agglutination test, respectively. Anti-S. neurona antibodies were found in 42.2% of the horses by using the SnSAG2 ELISA. Anti-Neospora spp. antibodies were found in only 3.5% of the horses by using the NhSAG1 ELISA, and only 1 of these horses was confirmed seropositive by Western blot. Antibodies to T gondii were found in 34.0% of the horses tested, which is higher than in previous reports from North and South America. The finding of anti-S. neurona antibodies in horses from geographical areas where Didelphis marsupials has wide distribution suggests that D. marsupialis is a potential definitive host for this parasite and a source of infection for these horses. C1 [Dangoudoubiyam, S.; Howe, D. K.] Univ Kentucky, Dept Vet Sci, Maxwell H Gluck Equine Res Ctr, Lexington, KY 40546 USA. [Oliveira, J. B.] Univ Fed Rural Pernambuco, Catedra Parasitol, Dept Biol, BR-52171900 Recife, PE, Brazil. [Oliveira, J. B.; Viquez, C.; Romero, J. J.] Univ Nacl Costa Rica, Escuela Med Vet, Heredia 863000, Apdo, Costa Rica. [Kwok, O. C. H.; Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dangoudoubiyam, S (reprint author), Univ Kentucky, Dept Vet Sci, Maxwell H Gluck Equine Res Ctr, Lexington, KY 40546 USA. EM dkhowe2@uky.edu NR 18 TC 17 Z9 17 U1 0 U2 5 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2011 VL 97 IS 3 BP 522 EP 524 DI 10.1645/GE-2722.1 PG 3 WC Parasitology SC Parasitology GA 779KA UT WOS:000291775300021 PM 21506839 ER PT J AU Hilali, M El-seify, M Zayed, A El-Morsey, A Dubey, JP AF Hilali, M. El-seify, M. Zayed, A. El-Morsey, A. Dubey, J. P. TI Sarcocystis dubeyi (Huong and Uggla, 1999) Infection in Water Buffaloes (Bubalus bubalis) from Egypt SO JOURNAL OF PARASITOLOGY LA English DT Article ID SP. PROTOZOA AB Water buffaloes (Bubalus bubalis) are intermediate hosts for 4 species of Sarcocystis, i.e. Sarcocystis fusiformis and Sarcocystis buffalonis with cats as definitive hosts; Sarcocystis levinei with dogs as definitive hosts, and Sarcocystis dubeyi with an unknown definitive host but thought to be zoonotic. Currently, the latter species has been identified with certainty only from Vietnam. In the present study, sarcocysts of S. dubeyi are reported in 11 (30%) of 35 Egyptian water buffaloes from which the esophageal muscles were examined histologically. Sarcocysts were microscopic, measuring 180-250 X 70-110 mu m in size. Ultrastructurally, the sarcocyst wall was 3.5-6.5 mu m thick and had palisade-like villar protrusions which give it a striated appearance. The villar protrusions contained microtubules that were distributed along the whole villus. This is the first report of S. dubeyi from water buffaloes in Egypt. C1 [Dubey, J. P.] ARS, Anim Parasit Dis Lab, USDA, Anim & Nat Resources Inst,BRC EAST, Beltsville, MD 20705 USA. [Hilali, M.] Cairo Univ, Dept Parasitol, Fac Vet Med, Giza 12211, Egypt. [El-seify, M.] Kafrelsheikh Univ, Dept Parasitol, Fac Vet Med, Kafrelsheikh, Egypt. [Zayed, A.] Natl Res Ctr, Parasitol & Anim Dis Dept, Vet Res Div, Giza 12311, Egypt. [El-seify, M.] Natl Res Ctr, Dept Parasitol, Cairo, Egypt. RP Dubey, JP (reprint author), ARS, Anim Parasit Dis Lab, USDA, Anim & Nat Resources Inst,BRC EAST, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Hilali, Mosaad/O-9443-2016; OI A. Hilali, Mosaad/0000-0002-9930-8501 NR 5 TC 6 Z9 6 U1 0 U2 0 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2011 VL 97 IS 3 BP 527 EP 528 DI 10.1645/GE-2656.1 PG 2 WC Parasitology SC Parasitology GA 779KA UT WOS:000291775300023 PM 21506768 ER PT J AU Gay, AN Lazar, DA Stoll, B Naik-Mathuria, B Mushin, OP Rodriguez, MA Burrin, DG Olutoye, OO AF Gay, Andre N. Lazar, David A. Stoll, Barbara Naik-Mathuria, Bindi Mushin, Oren P. Rodriguez, Manuel A. Burrin, Doug G. Olutoye, Oluyinka O. TI Near-infrared spectroscopy measurement of abdominal tissue oxygenation is a useful indicator of intestinal blood flow and necrotizing enterocolitis in premature piglets SO JOURNAL OF PEDIATRIC SURGERY LA English DT Article DE Necrotizing enterocolitis; NEC; Near infrared spectroscopy; NIRS; A-NIRS; StO(2); Premature; Piglets ID SPLANCHNIC PERFUSION EVALUATION; FED NEONATAL PIGLETS; PRETERM INFANTS; TRAUMA PATIENTS; EARLY TOLERANCE; RESUSCITATION; HEMORRHAGE; ABSORPTION; PREDICTION; SEVERITY AB Purpose: A major objective of necrotizing enterocolitis (NEC) research is to devise a noninvasive method of early detection. We hypothesized that abdominal near-infrared spectroscopy (A-NIRS) readings will identify impending NEC in a large animal model. Methods: Piglets were prematurely delivered and received parenteral nutrition followed by enteral feedings. Serial A-NIRS readings were obtained for 5 days, and animals were monitored for NEC. Separately, A-NIRS readings were obtained in healthy piglets to validate the correlation of A-NIRS with splanchnic oxygen delivery. Results: Of 29 piglets, 3 developed NEC. Eleven piglets without NEC died prematurely. Fifteen piglets remained healthy, had normal histologic assessment of their intestines, and served as controls. Abdominal near-infrared spectroscopy readings within 12 hours of birth were significantly lower in animals that developed NEC compared with healthy littermates (4% vs 33%, P = .02). For all time-points measured, A-NIRS readings were significantly lower in the NEC group compared with controls (21% vs 55%, P < .001). Abdominal near-infrared spectroscopy readings correlated with both decreased pulse oximetry readings during apneic episodes (r = 0.96) and increased superior mesenteric artery flow in response to glucagon-like peptide 2 (r = 0.67). Conclusion: Abdominal near-infrared spectroscopy is capable of detecting alterations in intestinal oxygenation and perfusion in neonatal piglets and may allow early detection of neonates at risk for NEC. (C) 2011 Elsevier Inc. All rights reserved. C1 [Gay, Andre N.; Lazar, David A.; Naik-Mathuria, Bindi; Mushin, Oren P.; Rodriguez, Manuel A.; Olutoye, Oluyinka O.] Texas Childrens Hosp, Baylor Coll Med, Michael E DeBakey Dept Surg, Div Pediat Surg, Houston, TX 77030 USA. [Stoll, Barbara; Burrin, Doug G.] ARS, Dept Pediat, Baylor Coll Med, USDA,Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Olutoye, OO (reprint author), Texas Childrens Hosp, Baylor Coll Med, Michael E DeBakey Dept Surg, Div Pediat Surg, 6701 Fannin St,CC 650-00, Houston, TX 77030 USA. EM oolutoye@bcm.edu FU US Department of Agriculture [58-6250-6-001]; Danish Research Councils (FOSU Committee); Texas Medical Center Digestive Diseases Center [P30 DK-56338]; National Institute of General Medical Sciences [GM-069912] FX This work was supported, in part, by federal funds from the US Department of Agriculture, under Cooperative Agreement 58-6250-6-001, the Danish Research Councils (FOSU Committee), the Texas Medical Center Digestive Diseases Center (National Institute of Diabetes and Digestive and Kidney Diseases Grant P30 DK-56338), and the National Institute of General Medical Sciences (GM-069912). The authors also thank Hutchinson Technology for use of the InSpectra StO2 Tissue Oxygenation Monitors. NR 32 TC 30 Z9 30 U1 0 U2 5 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0022-3468 J9 J PEDIATR SURG JI J. Pediatr. Surg. PD JUN PY 2011 VL 46 IS 6 BP 1034 EP 1040 DI 10.1016/j.jpedsurg.2011.03.025 PG 7 WC Pediatrics; Surgery SC Pediatrics; Surgery GA 778DD UT WOS:000291678700012 PM 21683194 ER PT J AU Carmo-Silva, AE Marri, L Sparla, F Salvucci, ME AF Carmo-Silva, A. Elizabete Marri, Lucia Sparla, Francesca Salvucci, Michael E. TI Isolation and Compositional Analysis of a CP12-Associated Complex of Calvin Cycle Enzymes from Nicotiana tabacum SO PROTEIN AND PEPTIDE LETTERS LA English DT Article DE Calvin Cycle; CP12; glyceraldehyde-3-phosphate dehydrogenase; phosphoribulokinase; protein-protein interactions; redox regulation ID PHOTOSYNTHETIC GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; RIBULOSE BISPHOSPHATE CARBOXYLASE; SUPRAMOLECULAR COMPLEX; ARABIDOPSIS-THALIANA; LIGHT REGULATION; PROTEIN CP12; CHLOROPLASTS; PHOSPHORIBULOKINASE; SPINACH; DISSOCIATION AB Two Calvin Cycle enzymes, NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK) form a multiprotein complex with CP12, a small intrinsically-unstructured protein. Under oxidizing conditions, association with CP12 confers redox-sensitivity to the otherwise redox-insensitive A isoform of GAPDH (GapA) and provides an additional level of down-regulation to the redox-regulated PRK. To determine if CP12mediated regulation is specific for GAPDH and PRK in vivo, a high molecular weight complex containing CP12 was isolated from tobacco chloroplasts and leaves and its protein composition was characterized. Gel electrophoresis and immunoblot analyses after separation of stromal proteins by size fractionation verified that the GAPDH (both isoforms) and PRK co-migrated with CP12 in dark-but not light-adapted chloroplasts. Nano-liquid-chromatography-mass-spectrometry of the isolated complex identified only CP12, GAPDH and PRK. Since nearly all of the CP12 from darkened chloroplasts migrates with GADPH and PRK as a high molecular mass species, these data indicate that the tight association of tobacco CP12 with GAPDH and PRK is specific and involves no other Calvin Cycle enzymes. C1 [Carmo-Silva, A. Elizabete; Salvucci, Michael E.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Marri, Lucia; Sparla, Francesca] Univ Bologna, Dept Expt Evolutionary Biol, Lab Mol Plant Physiol, I-40126 Bologna, Italy. RP Salvucci, ME (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM mike.salvucci@ars.usda.gov RI Carmo-Silva, Elizabete/G-7437-2012 OI Carmo-Silva, Elizabete/0000-0001-6059-9359 FU United States Department of Energy, Office of Basic Energy Sciences [DE-FG02-08ER20268, DEPS02-09ER09-02] FX The authors thank Dr. Dominique Rumeau (CNRS-CEA-Universite de la Mediterranee, France) for the generous gift of His-tag tobacco seeds, Professor Renate Scheibe (University of Osnabruck, Germany) for the generous gift of the GAPDH antibody, and Dr. Alfred Keys (Rothamsted Research, UK) for useful comments on the manuscript. The research was supported by the United States Department of Energy, Office of Basic Energy Sciences, Photosynthetic Systems Grants DE-FG02-08ER20268 and DEPS02-09ER09-02 (M.E.S.). NR 27 TC 11 Z9 11 U1 1 U2 10 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 0929-8665 J9 PROTEIN PEPTIDE LETT JI Protein Pept. Lett. PD JUN PY 2011 VL 18 IS 6 BP 618 EP 624 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 777QP UT WOS:000291639300011 PM 21271977 ER PT J AU Ye, XW Lai, CQ Crott, JW Troen, AM Ordovas, JM Tucker, KL AF Ye, Xingwang Lai, Chao-Qiang Crott, Jimmy W. Troen, Aron M. Ordovas, Jose M. Tucker, Katherine L. TI The Folate Hydrolase 1561C > T Polymorphism Is Associated With Depressive Symptoms in Puerto Rican Adults SO PSYCHOSOMATIC MEDICINE LA English DT Article DE depressive symptoms; genetics; polymorphisms; folate; folate hydrolase; glutamate carboxypeptidase II ID SINGLE-NUCLEOTIDE POLYMORPHISMS; GLUTAMATE-CARBOXYPEPTIDASE-II; NUTRITION EXAMINATION SURVEY; N-ACETYLASPARTYLGLUTAMATE; HORDALAND HOMOCYSTEINE; MAJOR DEPRESSION; CES-D; VITAMIN-B-12; HEALTH; SERUM AB Objective: To examine the associations between variants of genes involved in the uptake, retention, and metabolism of folate and depressive symptoms and to analyze whether such associations are direct or through mediation by folate or homocysteine. Methods: We performed a cross-sectional analysis of data from 976 Puerto Rican adults, aged 45 to 75 years, residing in the greater Boston area, Massachusetts. Twelve single nucleotide polymorphisms (SNPs) in genes involved in folate uptake, retention, and metabolism were investigated. These include FOLH1 (folate hydrolase), FPGS (folate polyglutamate synthase), GGH (gamma-glutamyl hydrolase), MTHFR (methylenetetrahydrofolate reductase), MTR (methionine synthase), PCFT (proton-coupled folate transporter), and RFC1 (reduced folate carrier 1). The Center for Epidemiologic Studies Depression Scale (CES-D) was used to measure depressive symptoms. Results: The FOLH1 rs61886492 C>T (or 1561C>T) polymorphism was significantly associated with lower CES-D score (p = .0025) after adjusting for age, sex, population admixture, smoking, and educational attainment. Individuals with the TT and TC genotypes were 49% less likely (odds ratio = 0.51, 95% confidence interval = 0.29-0.89) to report mild depressive symptoms (CES-D score >= 16 and <= 26) and 64% less likely. (odds ratio = 0.36, 95% confidence interval = 0.18-0.69) to report moderate to severe depressive symptoms (CES-D score >26), compared with those with the CC genotype. No significant mediation effects by plasma folate or homocysteine on the associations between this single nucleotide polymorphism and CES-D score were observed. Conclusions: The FOLH1 1561C>T polymorphism may be associated with the risk of depressive symptoms. C1 [Ye, Xingwang; Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Ye, Xingwang; Lai, Chao-Qiang; Crott, Jimmy W.; Troen, Aron M.; Ordovas, Jose M.; Tucker, Katherine L.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Boston, MA 02111 USA. RP Tucker, KL (reprint author), Northeastern Univ, Dept Hlth Sci, 316 Robinson Hall,360 Huntington Ave, Boston, MA 02115 USA. EM kl.tucker@neu.edu RI Tucker, Katherine/A-4545-2010; OI Tucker, Katherine/0000-0001-7640-662X; Ordovas, Jose/0000-0002-7581-5680 FU National Institutes of Health [P01 AG023394, P50 HL105185, R01 HL054776, R01 DK075030]; United States Department of Agriculture Research Service [58-1950-7-707, 58-1950-9-001, 53-K06-5-10] FX This research was supported by National Institutes of Health P01 AG023394 and P50 HL105185; R01 HL054776; R01 DK075030; and United States Department of Agriculture Research Service contracts 58-1950-7-707, 58-1950-9-001, and 53-K06-5-10. NR 48 TC 5 Z9 5 U1 1 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JUN PY 2011 VL 73 IS 5 BP 385 EP 392 DI 10.1097/PSY.0b013e31821a1ab4 PG 8 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA 780CG UT WOS:000291829300004 PM 21597034 ER PT J AU Milanovich, JR Trauth, SE Saugey, DA AF Milanovich, Joseph R. Trauth, Stanley E. Saugey, David A. TI REPRODUCTION AND AGE COMPOSITION OF A POPULATION OF WOODLAND SALAMANDERS (PLETHODON ALBAGULA) AFTER A PRESCRIBED BURN IN SOUTHWESTERN ARKANSAS SO SOUTHWESTERN NATURALIST LA English DT Article ID BLUE-RIDGE MOUNTAINS; TERRESTRIAL SALAMANDERS; FUEL REDUCTION; SMALL MAMMALS; CLEAR-CUT; FOREST; FIRE; AMPHIBIANS; CINEREUS; FOOD AB We studied a population of Plethodon albagula inhabiting an abandoned mine in Garland County, Arkansas, to test whether variables such as fecundity, diameter of egg, total number of eggs produced, and total number of clutches were influenced by precipitation or prescribed burning. We also compared ratios of juveniles to adults during summer, a period when P. albagula sought refuge in the mine, to determine if prescribed burning influenced certain life stages more than others. Comparing years before and after burning and accounting for the influence of precipitation, we did not detect a significant effect of the prescribed burn on any reproductive variable. However, we did discover a decline in some reproductive variables 1.5 years post-burning. There also was no decline in the ratio of juveniles to adults before or after the burn. Prescribed burning in the inactive season did not affect reproduction or age composition of the population of P. albagula. C1 [Milanovich, Joseph R.; Trauth, Stanley E.] Arkansas State Univ, Dept Biol Sci, State Univ, AR 72467 USA. [Saugey, David A.] US Forest Serv, Jessieville, AR 71949 USA. RP Milanovich, JR (reprint author), Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM milanovichj@warnell.uga.edu FU Arkansas State University [03-CS-11080901-090]; United States Department of Agriculture Forest Service (Ouachita National Forest) FX We are grateful to M. McCallum, R. Jordan, B. Wheeler, and many others for assistance in the field. We also thank R. B. Bury and J. W. Petranka for providing helpful comments on early drafts of the manuscript and J. C. Maerz, A. K. Davis, and K. Barrett for helpful advice. This study was conducted under authority of Arkansas Game and Fish Commission scientific permit (041420042) issued to SET. Funding was provided by a Challenge Cost-Share Agreement (03-CS-11080901-090) between Arkansas State University and the United States Department of Agriculture Forest Service (Ouachita National Forest). Appreciation is extended to personnel of the United States Army Corps of Engineers, Lake Ouachita Field Office, for allowing access and entry into the mine and to personnel of the United States Forest Service for conducting the prescribed burn. NR 49 TC 1 Z9 1 U1 1 U2 8 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN PY 2011 VL 56 IS 2 BP 172 EP 179 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 779IX UT WOS:000291772400005 ER PT J AU Block, WM Russell, RE Ganey, JL AF Block, William M. Russell, Robin E. Ganey, Joseph L. TI OCCUPANCYAND HABITAT ASSOCIATIONS OF FOUR SPECIES OF SCIURIDS IN PONDEROSA PINE-GAMBEL OAK (PINUS PONDEROSA-QUERCUS GAMBELII) FORESTS IN NORTHERN ARIZONA SO SOUTHWESTERN NATURALIST LA English DT Article ID MIXED-CONIFER FOREST; PRESCRIBED FIRE; ECOLOGICAL RESTORATION; ABERT SQUIRREL; WOODY DEBRIS; HOME-RANGE; POPULATION; PRINCIPLES; MANAGEMENT; WILDLIFE AB In ponderosa pine-Gambel oak (Pinus ponderosa-Quercus gambelii) forests of north-central Arizona, we examined occupancy and habitats of four species of sciurids (rock squirrel Spermophilus variegatus, golden-mantled ground squirrel S. lateralis, gray-collared chipmunk Tamias cinereicollis, cliff chipmunk T dorsalis). Numbers of captures were highly variable, suggesting a patchy distribution of species. All four species co-occurred on only one of the study sites. Gray-collared chipmunks and golden-mantled ground squirrels were relatively common on one site only. Golden-mantled ground squirrels were associated with mature forests with high canopy cover and low basal area, whereas gray-collared chipmunks were in locations with large numbers of logs and shallow litter. The two species of chipmunks appeared to be separated in space; gray-collared chipmunks were more common where cliff chipmunks were least common. Rock squirrels were relatively ubiquitous. There was evidence for an association of rock squirrels with increasing rocky cover and decreasing canopy cover, whereas cliff chipmunks occupied rocky areas with high canopy cover and numerous shrubs. C1 [Block, William M.; Ganey, Joseph L.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Russell, Robin E.] US Forest Serv, Rocky Mt Res Stn, Bozeman, MT 59717 USA. RP Block, WM (reprint author), US Forest Serv, Rocky Mt Res Stn, SW Forest Sci Complex,2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM wblock@fs.fed.us OI Russell, Robin/0000-0001-8726-7303 FU Rocky Mountain Research Station of the United States Forest Service; Southwestern Region of the United States Forest Service FX We thank S. Converse, M. Seamans, and two anonymous reviewers for insightful reviews that improved the manuscript. We also thank J. Iniguez and C. Cones Montano for translating the abstract into Spanish. Funding was provided by Rocky Mountain Research Station and the Southwestern Region, both of the United States Forest Service. Numerous people assisted with collection of data including K. Berger, J. L. Block, L. DiDonato, S. J. Jenness, S. Miller, D. Olson, M. Morrison, H. W. Range, P. Stapp, P. Stefanek, and B. Strohmeyer. S. DeRosier, J. Jenness, S. Miller, B. Stohmeyer, and G. Wildeman assisted with entry of data, and J. Jenness assisted in debugging the files. NR 47 TC 0 Z9 0 U1 0 U2 19 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 EI 1943-6262 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN PY 2011 VL 56 IS 2 BP 193 EP 203 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 779IX UT WOS:000291772400008 ER PT J AU Griffs-Kyle, KL Kyle, S Jungels, J AF Griffs-Kyle, Kerry L. Kyle, Sean Jungels, Jeremy TI USE OF BREEDING SITES BY ARID-LAND TOADS IN RANGELANDS: LANDSCAPE-LEVEL FACTORS SO SOUTHWESTERN NATURALIST LA English DT Article ID CONSERVATION; ABUNDANCE; ENVIRONMENTS; OCCUPANCY AB We examined spatial extent of habitat that anaxyrids responded to in an arid environment. We used surveys of vocalizations and searches to identify toads after rainfall events to examine whether the spatial arrangement and proximity of earthen tanks could influence breeding populations of Anaxyrus cognatus and A. These species responded to the landscape complement of breeding sites inside a buffer of 5 km, a much larger distance than most studies have addressed. C1 [Griffs-Kyle, Kerry L.] Texas Tech Univ, Dept Nat Resources Management, Lubbock, TX 79409 USA. [Kyle, Sean] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86017 USA. [Jungels, Jeremy] New Mexico State Univ, Dept Fish Wildlife & Conservat Ecol, Las Cruces, NM 88011 USA. RP Griffs-Kyle, KL (reprint author), Texas Tech Univ, Dept Nat Resources Management, Box 42125, Lubbock, TX 79409 USA. EM keny.griffis-kyle@ttu.edu NR 21 TC 3 Z9 3 U1 1 U2 8 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN PY 2011 VL 56 IS 2 BP 251 EP 255 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 779IX UT WOS:000291772400016 ER PT J AU Pech, LL Gates, MW Graham, TB AF Pech, Louis L. Gates, Michael W. Graham, Tim B. TI DIRHINUS TEXANUS (HYMENOPTERA: CHALCIDIDAE) FROM UTAH SO SOUTHWESTERN NATURALIST LA English DT Article ID BIOTIC INVENTORIES; NATIONAL-PARKS AB We collected a Dirhinus texanus (Hymenoptera: Chalcididae) in Salt Creek Canyon, Canyonlands National Park, an Juan County, Utah. This is the first record for D. texanus in Utah. C1 [Pech, Louis L.] Univ Wisconsin Marathon Cty, Dept Biol Sci, Wausau, WI 54401 USA. [Gates, Michael W.] ARS, Systemat Entomol Lab, USDA, Plant Sci Inst,Natl Museum Nat Hist, Washington, DC 20013 USA. [Graham, Tim B.] US Geol Survey, Biol Resource Div, Moab, UT 84532 USA. RP Pech, LL (reprint author), Univ Wisconsin Marathon Cty, Dept Biol Sci, 518 S 7th Ave, Wausau, WI 54401 USA. EM lou.pech@uwc.edu NR 9 TC 0 Z9 0 U1 1 U2 2 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN PY 2011 VL 56 IS 2 BP 275 EP 276 PG 2 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 779IX UT WOS:000291772400022 ER PT J AU Costanzo, S Jackson, AK Brooks, SA AF Costanzo, Stefano Jackson, Aaron K. Brooks, Steven A. TI High-resolution mapping of Rsn1, a locus controlling sensitivity of rice to a necrosis-inducing phytotoxin from Rhizoctonia solani AG1-IA SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID SHEATH BLIGHT RESISTANCE; QUANTITATIVE TRAIT LOCI; ORYZA-SATIVA L.; UDP-GLUCOSYLTRANSFERASE; ARABIDOPSIS-THALIANA; BIOLOGICAL-ACTIVITY; JASMINE 85; CYTOKININS; GENE; QTLS AB Rhizoctonia solani is a necrotrophic fungal pathogen that causes disease on many crop-plant species. Anastomosis group 1-IA is the causal agent of sheath blight of rice (Oryza sativa L.), one of the most important rice diseases worldwide. R. solani AG1-IA produces a necrosis-inducing phytotoxin and rice cultivar's sensitivity to the toxin correlates with disease susceptibility. Unlike genetic analyses of sheath blight resistance where resistance loci have been reported as quantitative trait loci, phytotoxin sensitivity is inherited as a Mendelian trait that permits high-resolution mapping of the sensitivity genes. An F-2 mapping population derived from parent cultivars 'Cypress' (toxin sensitive) and 'Jasmine 85' (toxin insensitive) was used to map Rsn1, the necrosis-inducing locus. Initial mapping based on 176 F-2 progeny and 69 simple sequence repeat (SSR) markers located Rsn1 on the long arm of chromosome 7, with tight linkage to SSR marker RM418. A high-resolution genetic map of the region was subsequently developed using a total of 1,043 F-2 progeny, and Rsn1 was mapped to a 0.7 cM interval flanked by markers NM590 and RM418. Analysis of the corresponding 29 Kb genomic sequences from reference cultivars 'Nipponbare' and '93-11' revealed the presence of four putative genes within the interval. Two are expressed cytokinin-O-glucosyltransferases, which fit an apoptotic pathway model of toxin activity, and are individually being investigated further as potential candidates for Rsn1. C1 [Costanzo, Stefano; Jackson, Aaron K.; Brooks, Steven A.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Brooks, SA (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 E, Stuttgart, AR 72160 USA. EM ricegenes@mac.com FU USDA Cooperative State Research, Education and Extension Service-National Research Initiative [2004-35317-14867]; USDA ARS FX This work was supported in part by the USDA Cooperative State Research, Education and Extension Service-National Research Initiative-Applied Plant Genomics Program entitled "RiceCAP: A coordinated research, education, and extension project for the application of genomic discoveries to improve rice in the United States" (USDA/CSREES grant 2004-35317-14867). Additional funding was also provided by USDA ARS through a post-doctoral research associate grant. The authors gratefully acknowledge Gordon Miller, Tara Moss and Melissa Jia for excellent technical support. NR 48 TC 6 Z9 6 U1 2 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2011 VL 123 IS 1 BP 33 EP 41 DI 10.1007/s00122-011-1564-1 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 777FC UT WOS:000291600300004 PM 21424397 ER PT J AU Andru, S Pan, YB Thongthawee, S Burner, DM Kimbeng, CA AF Andru, Suman Pan, Yong-Bao Thongthawee, Songkran Burner, David M. Kimbeng, Collins A. TI Genetic analysis of the sugarcane (Saccharum spp.) cultivar 'LCP 85-384'. I. Linkage mapping using AFLP, SSR, and TRAP markers SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID RUST RESISTANCE GENE; MICROSATELLITE MARKERS; GENOME STRUCTURE; MAP; SEGREGATION; OFFICINARUM; SPONTANEUM; ORGANIZATION; L.; IDENTIFICATION AB Sugarcane hybrids are complex aneu-polyploids (2n = 100-130) derived from inter-specific hybridization between ancestral polyploid species, namely S. officinarum L. and S. spontaneum L. Efforts to understand the sugarcane genome have recently been enhanced through the use of new molecular marker technologies. A framework genetic linkage map of Louisiana's popular cultivar LCP 85-384 was constructed using the selfed progeny and based on polymorphism derived from 64 AFLP, 19 SSR and 12 TRAP primer pairs. Of 1,111 polymorphic markers detected, 773 simplex (segregated in 3:1 ratio) and 182 duplex (segregate in 77:4 ratio) markers were used to construct the map using a LOD value of >= 4.0 and recombination threshold of 0.44. The genetic distances between pairs of markers linked in the coupling phase was computed using the Kosambi mapping function. Of the 955 markers, 718 simplex and 66 duplex markers were assigned to 108 co-segregation groups (CGs) with a cumulative map length of 5,617 cM and a density of 7.16 cM per marker. Fifty-five simplex and 116 duplex markers remained unlinked. With an estimated genome size of 12,313 cM for LCP 85-384, the map covered approximately 45.6% of the genome. Forty-four of the 108 CGs were assigned into 9 homo(eo)logous groups (HGs) based on information from locus-specific SSR and duplex markers, and repulsion phase linkages detected between CGs. Meiotic behavior of chromosomes in cytogenetic studies and repulsion phase linkage analysis between CGs in this study inferred the existence of strong preferential chromosome pairing behavior in LCP 85-384. This framework map marks an important beginning for future mapping of QTLs associated with important agronomic traits in the Louisiana sugarcane breeding programs. C1 [Kimbeng, Collins A.] Louisiana State Univ, Sugar Res Stn, Ctr Agr, St Gabriel, LA 70776 USA. [Andru, Suman] Louisiana State Univ, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Pan, Yong-Bao] USDA ARS, Sugarcane Res Lab, MSA, Houma, LA 70360 USA. [Thongthawee, Songkran] Kasetsart Univ, Ctr Agr Biotechnol, Nakhon Pathom 73140, Thailand. [Burner, David M.] USDA ARS, SPA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. RP Kimbeng, CA (reprint author), Louisiana State Univ, Sugar Res Stn, Ctr Agr, St Gabriel, LA 70776 USA. EM ckimbeng@agctr.lsu.edu FU American Sugar Cane League of the USA, Inc.; School of Plant, Environmental and Soil Sciences, Louisiana State University FX We thank Johan W. Van Ooijen for his kind assistance on how best to use the JoinMap 3.0 software in mapping a polyploid species. We also appreciate Anudeep Paturi, Lionel Lomax, and Jennifer Shaw for their immense help in this project. Financial support from the American Sugar Cane League of the USA, Inc., and the School of Plant, Environmental and Soil Sciences, Louisiana State University are gratefully acknowledged. NR 61 TC 22 Z9 25 U1 3 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2011 VL 123 IS 1 BP 77 EP 93 DI 10.1007/s00122-011-1568-x PG 17 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 777FC UT WOS:000291600300008 PM 21472411 ER PT J AU Bushman, BS Larson, SR Tuna, M West, MS Hernandez, AG Vullaganti, D Gong, G Robins, JG Jensen, KB Thimmapuram, J AF Bushman, B. Shaun Larson, Steve R. Tuna, Metin West, Mark S. Hernandez, Alvaro G. Vullaganti, Deepika Gong, George Robins, Joseph G. Jensen, Kevin B. Thimmapuram, Jyothi TI Orchardgrass (Dactylis glomerata L.) EST and SSR marker development, annotation, and transferability SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GENETIC-LINKAGE MAP; LOLIUM-PERENNE L.; MICROSATELLITE MARKERS; SIMILARITY INDEX; GENUS DACTYLIS; DIVERSITY; WHEAT; SEQUENCES; BARLEY; RICE AB Orchardgrass, or cocksfoot [Dactylis glomerata (L.)], has been naturalized on nearly every continent and is a commonly used species for forage and hay production. All major cultivated varieties of orchardgrass are autotetraploid, and few tools or information are available for functional and comparative genetic analyses and improvement of the species. To improve the genetic resources for orchardgrass, we have developed an EST library and SSR markers from salt, drought, and cold stressed tissues. The ESTs were bi-directionally sequenced from clones and combined into 17,373 unigenes. Unigenes were annotated based on putative orthology to genes from rice, Triticeae grasses, other Poaceae, Arabidopsis, and the non-redundant database of the NCBI. Of 1,162 SSR markers developed, approximately 80% showed amplification products across a set of orchardgrass germplasm, and 40% across related Festuca and Lolium species. When orchardgrass subspecies were genotyped using 33 SSR markers their within-accession similarity values ranged from 0.44 to 0.71, with Mediterranean accessions having a higher similarity. The total number of genotyped bands was greater for tetraploid accessions compared to diploid accessions. Clustering analysis indicated grouping of Mediterranean subspecies and central Asian subspecies, while the D. glomerata ssp. aschersoniana was closest related to three cultivated varieties. C1 [Bushman, B. Shaun; Larson, Steve R.; Robins, Joseph G.; Jensen, Kevin B.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [West, Mark S.] USDA ARS, Ft Collins, CO 80526 USA. [Hernandez, Alvaro G.; Vullaganti, Deepika; Gong, George; Thimmapuram, Jyothi] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. [Tuna, Metin] Namik Kemal Univ, Dept Field Crops, Fac Agr, TR-59100 Tekirdag, Turkey. RP Bushman, BS (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Lab, 695 N 1100 E, Logan, UT 84322 USA. EM shaun.bushman@ars.usda.gov OI Larson, Steve/0000-0003-2742-2134 NR 42 TC 21 Z9 28 U1 3 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2011 VL 123 IS 1 BP 119 EP 129 DI 10.1007/s00122-011-1571-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 777FC UT WOS:000291600300011 PM 21465186 ER PT J AU Lowe, I Jankuloski, L Chao, SM Chen, XM See, D Dubcovsky, J AF Lowe, Iago Jankuloski, Ljupcho Chao, Shiaoman Chen, Xianming See, Deven Dubcovsky, Jorge TI Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; PLANT DISEASE RESISTANCE; PUCCINIA-STRIIFORMIS; UNITED-STATES; COMMON WHEAT; TETRAPLOID WHEAT; BREAD WHEAT; AESTIVUM L.; GENE SR2; MARKERS AB A mapping population of 186 recombinant inbred lines developed from a cross between UC1110, an adapted California spring wheat, and PI610750, a synthetic derivative from CIMMYT's Wide Cross Program, was evaluated for its response to current California races of stripe rust (Puccinia striiformis f. sp. tritici) in replicated field trials over four seasons (2007-2010) in the northern Sacramento Valley. A genetic map was constructed consisting of 1,494 polymorphic probes (SSRs, DArTs, and ESTs) mapped to 558 unique loci, and QTL analysis revealed the presence of four stripe rust resistance QTL segregating in this population, two from UC1110 (on chromosomes 3BS and 2BS) and two from PI610750 (5AL and 2AS). The two QTL of largest effects (on 3BS and 5AL) were validated in independent populations and their intervals narrowed to 2.5 and 5.3 cM, respectively. The 3BS QTL was shown, by allelism test and genotype, to carry a gene different from the Yr30/Sr2 complex. Mapped position also suggests that the 3BS QTL is associated with a gene different from either Yrns-B1 or YrRub, two stripe rust resistance genes mapped to this region in other studies. The 5AL QTL carries a previously unreported partial stripe rust resistance gene, designated here as Yr48. This paper discusses the individual contributions to resistance of these four QTL, their epistatic interactions, and their potential in durable resistance breeding strategies based on combinations of partial resistance genes. C1 [Lowe, Iago; Jankuloski, Ljupcho; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Jankuloski, Ljupcho] Fac Agr Sci & Food, Dept Genet & Plant Breeding, Skopje 1000, Macedonia. [Chao, Shiaoman] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Chen, Xianming; See, Deven] Washington State Univ, USDA ARS, Dis Res Unit, Pullman, WA 99164 USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, 1 Shields Av, Davis, CA 95616 USA. EM jdubcovsky@ucdavis.edu RI Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU USDA National Institute of Food and Agriculture [2009-65300-05640, 2011-68002-30029 (Triticeae-CAP)]; UC Davis Department of Plant Sciences; International Atomic Energy Agency FX This project was supported by the National Research Initiative Competitive Grants 2009-65300-05640 and 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. I. Lowe received valuable support through the Graduate Student Research Fellowship program of the UC Davis Department of Plant Sciences. L. Jankuloski received fellowship support from the 4International Atomic Energy Agency. The authors thank O. Chicaiza, Z. Abate, A. Distelfeld, D. Fu, F. Paraiso, C. Uauy, A. Wan, D. Feltus, M. Osenga, V. Talbott, and especially X. Zhang for excellent technical assistance. NR 44 TC 40 Z9 44 U1 3 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2011 VL 123 IS 1 BP 143 EP 157 DI 10.1007/s00122-011-1573-0 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 777FC UT WOS:000291600300013 PM 21455722 ER PT J AU Qi, LL Pumphrey, MO Friebe, B Zhang, P Qian, C Bowden, RL Rouse, MN Jin, Y Gill, BS AF Qi, L. L. Pumphrey, M. O. Friebe, Bernd Zhang, P. Qian, C. Bowden, R. L. Rouse, M. N. Jin, Y. Gill, B. S. TI A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; CONFERRING RESISTANCE; PSEUDOCERCOSPORELLA-HERPOTRICHOIDES; 1RS.1BL TRANSLOCATION; CENTRIC MISDIVISION; CHROMOSOMES; CENTROMERES; LINES; TTKS; RECOMBINATION AB Stem rust (Puccinia graminis f. sp. tritici Eriks. & E. Henn.) (the causal agent of wheat stem rust) race Ug99 (also designated TTKSK) and its derivatives have defeated several important stem rust resistance genes widely used in wheat (Triticum aestivum L.) production, rendering much of the worldwide wheat acreage susceptible. In order to identify new resistance sources, a large collection of wheat relatives and genetic stocks maintained at the Wheat Genetic and Genomic Resources Center was screened. The results revealed that most accessions of the diploid relative Dasypyrum villosum (L.) Candargy were highly resistant. The screening of a set of wheat-D. villosum chromosome addition lines revealed that the wheat-D. villosum disomic addition line DA6V#3 was moderately resistant to race Ug99. The objective of the present study was to produce and characterize compensating wheat-D. villosum whole arm Robertsonian translocations (RobTs) involving chromosomes 6D of wheat and 6V#3 of D. villosum through the mechanism of centric breakage-fusion. Seven 6V#3-specific EST-STS markers were developed for screening F(2) progeny derived from plants double-monosomic for chromosomes 6D and 6V#3. Surprisingly, although 6D was the target chromosome, all recovered RobTs involved chromosome 6A implying a novel mechanism for the origin of RobTs. Homozygous translocations (T6AS.6V#3L and T6AL.6V#3S) with good plant vigor and full fertility were selected from F(3) families. A stem rust resistance gene was mapped to the long arm 6V#3L in T6AS.6V#3L and was designated as Sr52. Sr52 is temperature-sensitive and is most effective at 16 degrees C, partially effective at 24 degrees C, and ineffective at 28 degrees C. The T6AS.6V#3L stock is a new source of resistance to Ug99, is cytogenetically stable, and may be useful in wheat improvement. C1 [Friebe, Bernd; Qian, C.; Gill, B. S.] Kansas State Univ, Dept Plant Pathol, Wheat Genet & Genom Resources Ctr, Manhattan, KS 66506 USA. [Qi, L. L.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Pumphrey, M. O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Zhang, P.] Univ Sydney, Plant Breeding Inst, Camden, NSW 2570, Australia. [Bowden, R. L.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Rouse, M. N.; Jin, Y.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Friebe, B (reprint author), Kansas State Univ, Dept Plant Pathol, Wheat Genet & Genom Resources Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. EM friebe@ksu.edu RI Rouse, Matthew/G-5474-2011; Bowden, Robert/E-3268-2013 OI Bowden, Robert/0000-0001-9419-6574 FU Kansas Wheat Commission; Kansas Crop Improvement Association; USDA-CSREES; Genomic Resources Center; Cornell University FX We thank Drs. Brady Vick and Steven S. Xu for critical review of the manuscript, W. John Raupp for editorial assistance with the manuscript, and Duane L. Wilson for excellent technical help. This research was supported by grants from the Kansas Wheat Commission, the Kansas Crop Improvement Association, a special USDA-CSREES grant to the Wheat Genetic and Genomic Resources Center, and a grant from Cornell University Project "Durable Rust Resistance in Wheat". This paper is contribution number 11-166-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502. NR 44 TC 49 Z9 56 U1 0 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2011 VL 123 IS 1 BP 159 EP 167 DI 10.1007/s00122-011-1574-z PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 777FC UT WOS:000291600300014 PM 21437597 ER PT J AU Flather, CH Hayward, GD Beissinger, SR Stephens, PA AF Flather, Curtis H. Hayward, Gregory D. Beissinger, Steven R. Stephens, Philip A. TI Minimum viable populations: is there a 'magic number' for conservation practitioners? SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Review ID VIABILITY ANALYSIS; EXTINCTION RISK; BODY-SIZE; BIOLOGY; WILDLIFE; BIODIVERSITY; PROBABILITY; UNCERTAINTY; MEANINGFUL; MANAGEMENT AB Establishing species conservation priorities and recovery goals is often enhanced by extinction risk estimates. The need to set goals, even in data-deficient situations, has prompted researchers to ask whether general guidelines could replace individual estimates of extinction risk. To inform conservation policy, recent studies have revived the concept of the minimum viable population (MVP), the population size required to provide some specified probability of persistence for a given period of time. These studies conclude that long-term persistence requires >= 5000 adult individuals, an MVP threshold that is unaffected by taxonomy, life history or environmental conditions. Here, we re-evaluate this suggestion. We find that neither data nor theory supports its general applicability, raising questions about the utility of MVPs for conservation planning. C1 [Stephens, Philip A.] Univ Durham, Sch Biol & Biomed Sci, Durham DH1 3LE, England. [Flather, Curtis H.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Hayward, Gregory D.] US Forest Serv, USDA, Anchorage, AK 99503 USA. [Hayward, Gregory D.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Beissinger, Steven R.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Stephens, PA (reprint author), Univ Durham, Sch Biol & Biomed Sci, South Rd, Durham DH1 3LE, England. EM philip.stephens@durham.ac.uk RI Beissinger, Steven/F-3809-2012; Stephens, Philip/B-8397-2008; Flather, Curtis/G-3577-2012 OI Stephens, Philip/0000-0001-5849-788X; Flather, Curtis/0000-0002-0623-3126 FU USDA Forest Service FX We thank Rudy King, John McNamara and Shane Richards for statistical advice, and Jonathan Rhodes, Chris Carbone, Barry Brook, Mark Boyce and Barry Noon for insightful comments on earlier drafts. P.A.S. was supported, in part, by funding from the USDA Forest Service International Programs. NR 84 TC 63 Z9 68 U1 14 U2 145 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD JUN PY 2011 VL 26 IS 6 BP 307 EP 316 DI 10.1016/j.tree.2011.03.001 PG 10 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 780HA UT WOS:000291843900011 PM 21458878 ER PT J AU Carpenter, JP Wang, Y Schweitzer, C Hamel, PB AF Carpenter, John P. Wang, Yong Schweitzer, Callie Hamel, Paul B. TI AVIAN COMMUNITY AND MICROHABITAT ASSOCIATIONS OF CERULEAN WARBLERS IN ALABAMA SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article ID EASTERN ONTARIO; HABITAT CHARACTERISTICS; REPRODUCTIVE SUCCESS; BREEDING BIRDS; ABUNDANCE; SELECTION; CONSERVATION; DISTURBANCE; ORDINATION; VIRGINIA AB Cerulean Warblers (Dendroica cerulea) have experienced one of the highest population declines of any neotropical-Nearctic migratory species in North America. We performed point counts and habitat assessments in areas used and unused by Cerulean Warblers in northern Alabama during the 2005 and 2006 breeding seasons to examine their avian associations and identify microhabitat features that best explained their occurrence. We detected on average similar to 50 Cerulean Warbler males (total) in three disjunct populations during each breeding season. Areas used by Cerulean Warblers were characterized by avian communities with significantly higher species richness, diversity, and abundance compared to areas where they were not detected. Correspondence analysis related Cerulean Warblers to inhabitants of riparian, bottomland deciduous forests (e.g., Kentucky Warbler [Oporornis formosus] Acadian Flycatcher [Empidonax virescens], and Northern Parula [Panda americana]) and two edge specialists (Blue-winged Warbler [Vermivora cyanoptera] and Indigo Bunting [Passerine cyanea]) suggesting Cerulean Warblers in our study areas may be tolerant of some habitat disturbance within an otherwise largely forested landscape. Information theoretic criteria and canonical correspondence analysis indicated Cerulean Warblers preferred bottomland forests containing tall (> 29 m), large diameter, well-spaced (> 27 m(2)/ha) deciduous trees with greater canopy cover (>= 90%), closer (< 20 m) canopy gaps, fewer snags (<= 25/ha), and a moderately complex canopy structure. Received 5 March 2010. Accepted 22 December 2010. C1 [Carpenter, John P.; Wang, Yong] Alabama A&M Univ, Dept Nat Resources & Environm Sci, Normal, AL 35762 USA. [Schweitzer, Callie] US Forest Serv, USDA, So Res Stn, Normal, AL 35762 USA. [Hamel, Paul B.] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. RP Carpenter, JP (reprint author), N Carolina Wildlife Resources Commiss, 1751 Vars Dr, Raleigh, NC 27606 USA. EM john.carpenter@ncwildlife.org FU Alabama A&M University, Alabama Department of Conservation and Natural Resources, U.S. Forest Service; U.S. Fish and Wildlife Service FX Funding for this project was provided by Alabama A&M University, Alabama Department of Conservation and Natural Resources, U.S. Forest Service, and U.S. Fish and Wildlife Service. We are grateful to E. C. Soehren, A. A. Lesak, C. M. Kilgore, and L. M. Gardner-Barillas for field assistance; J. A. Cochran, T. U. Counts, and G. M. Lein for logistical support; R. L. West and T. M. Haggerty for historic Cerulean Warbler records and Breeding Bird Atlas data; and W. B. Tadesse and K. E. Ward for suggestions that improved the thesis on which this study is based. This manuscript benefited greatly from comments provided by Jason Jones, C. E. Braun, and two anonymous reviewers. This project would have been impossible without the cooperation of many private landowners who granted access to their property. We especially thank the Cagle and Miller families, and the Stevenson Land Company. NR 66 TC 5 Z9 6 U1 6 U2 27 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD JUN PY 2011 VL 123 IS 2 BP 206 EP 217 PG 12 WC Ornithology SC Zoology GA 779KK UT WOS:000291776300002 ER PT J AU Chacko, SK Ordonez, J Sauer, PJJ Sunehag, AL AF Chacko, Shaji K. Ordonez, Jorge Sauer, Pieter J. J. Sunehag, Agneta L. TI Gluconeogenesis is Not Regulated by Either Glucose or Insulin in Extremely Low Birth Weight Infants Receiving Total Parenteral Nutrition SO JOURNAL OF PEDIATRICS LA English DT Article ID CARBOHYDRATE-METABOLISM; PREMATURE-INFANTS; PHYSIOLOGICAL HYPERCORTISOLEMIA; PROTEIN-SYNTHESIS; PLASMA-CORTISOL; NEWBORN LAMB; INFUSION; HYPOGLYCEMIA; HUMANS; PROTEOLYSIS AB Objective To determine potential factors regulating gluconeogenesis (GNG) in extremely low birth weight infants receiving total parenteral nutrition. Study design Seven infants (birth weight, 0.824 +/- 0.068 kg; gestational age, 25.4 +/- 0.5 weeks; postnatal age, 3.3 +/- 0.2 days) were studied for 11 hours, with parenteral lipid and amino acid therapy continued at prestudy rates. Glucose was supplied at prestudy rates for the first 5 hours (period 1) and was then reduced to 6 mg/kg.min for 1 hour and further to similar to 3 mg/kg.min for 5 hours (period 2). A total of 2.5 mg/kg.min of the glucose was replaced by [U-C-13] glucose throughout the study for measurements of glucose production and GNG. Concentrations of glucose, insulin, glucagons, and cortisol were determined. Results GNG and glucose production remained unchanged (2.12 +/- 0.23 vs. 1.84 +/- 0.25 mg/kg.min [P = NS] and 2.44 +/- 0.27 vs. 2.51 +/- 0.31 mg/kg.min [P = NS], respectively), despite a 60% reduction of the glucose infusion rate and subsequent 30% (124.7 +/- 10.8 to 82.6 +/- 8.9 mg/dL; P = .009) and 70% (26.9 +/- 4.7 to 6.6 +/- 0.4 mU/mL; P = .002) decreases in glucose and insulin concentrations, respectively. Cortisol and glucagon concentrations remained unchanged. Conclusion In extremely low birth weight infants receiving total parenteral nutrition, GNG is a continuous process that is not affected by infusion rates of glucose or concentrations of glucose or insulin. (J Pediatr 2011;158:891-6). C1 [Chacko, Shaji K.; Ordonez, Jorge; Sunehag, Agneta L.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Sauer, Pieter J. J.] Univ Groningen, Dept Pediat, Beatrix Childrens Hosp, Univ Med Ctr Groningen, NL-9700 AB Groningen, Netherlands. RP Sunehag, AL (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. EM asunehag@bcm.tmc.edu FU National Institutes of Health [RO1 HD 37857]; US Department of Agriculture [58-6250-6-001]; General Clinical Research Center, National Center for Research Resources [NIH MO1-RR-001888] FX Funded by the National Institutes of Health (Grant RO1 HD 37857), US Department of Agriculture (Cooperative Agreement 58-6250-6-001), and General Clinical Research Center, National Center for Research Resources (Grant NIH MO1-RR-001888). The contents of this article do not necessarily reflect the views of policies of the US Department of Agriculture, nor does any mention of trade names, commercial products, or organizations imply endorsement from the US Government. The authors declare no conflicts of interest. NR 41 TC 14 Z9 14 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 J9 J PEDIATR-US JI J. Pediatr. PD JUN PY 2011 VL 158 IS 6 BP 891 EP 896 DI 10.1016/j.jpeds.2010.12.040 PG 6 WC Pediatrics SC Pediatrics GA 763LQ UT WOS:000290558600008 PM 21324479 ER PT J AU McCulloh, KA Johnson, DM Meinzer, FC Lachenbruch, B AF McCulloh, Katherine A. Johnson, Daniel M. Meinzer, Frederick C. Lachenbruch, Barbara TI AN ANNUAL PATTERN OF NATIVE EMBOLISM IN UPPER BRANCHES OF FOUR TALL CONIFER SPECIES SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE Abies grandis; hydraulic conductivity; Pseudotsuga menziesii; Thuja plicata; Tsuga heterophylla; wood density ID SEMPERVIRENS D. DON; FREEZE-THAW CYCLES; PINUS-SYLVESTRIS L; XYLEM EMBOLISM; HYDRAULIC CONDUCTIVITY; WATER RELATIONS; ALPINE TIMBERLINE; NEGATIVE-PRESSURE; NORWAY SPRUCE; STONE PINE AB Premise of the study: The Pacific Northwest of North America experiences relatively mild winters and dry summers. For the tall coniferous trees that grow in this region, we predicted that loss in the hydraulic conductivity of uppermost branches would be avoided because of difficulty reversing accumulated emboli in xylem that is always under negative pressure. Methods: To test this hypothesis, we measured native percent loss in hydraulic conductivity (PLC; the decrease of in situ hydraulic conductivity relative to the maximum) monthly throughout 2009 in branches at the tops (similar to 50 m) of four species in an old growth forest in southern Washington. Key results: Contrary to our prediction, freeze-thaw cycles resulted in considerable native PLC. Branches showed hydraulic recovery in the spring and after a moderate increase in native embolism that was observed after an unusually hot period in August. The September recovery occurred despite decreases in the leaf and stem water potentials compared to August values. Conclusions: Recoveries in branches of these trees could not have occurred by raising the water potential enough to dissolve bubbles simply by transporting water from roots and must have occurred either through water absorption through needles and/or refilling under negative pressure. Excluding the August value, native embolism values correlated strongly with air temperature of the preceding 10 d. For three species, we found that branches with lower wood density had higher specific conductivity, but not greater native PLC than branches with higher wood density, which calls into question whether there is any hydraulic benefit to higher wood density in small branches in those species. C1 [McCulloh, Katherine A.; Lachenbruch, Barbara] Oregon State Univ, Dept Wood Sci & Engn, Corvallis, OR 97331 USA. [Johnson, Daniel M.; Meinzer, Frederick C.] US Forest Serv, USDA, PNW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. RP McCulloh, KA (reprint author), Oregon State Univ, Dept Wood Sci & Engn, Corvallis, OR 97331 USA. EM kate.mcculloh@oregonstate.edu RI Johnson, Daniel/E-6789-2011; Meinzer, Frederick/C-3496-2012 OI Johnson, Daniel/0000-0001-5890-3147; FU National Science Foundation [IBN 09-19871] FX This project was funded by National Science Foundation grant IBN 09-19871. We are very grateful to L. Bernart, M. Loloum, and S. du Peloux du Saint Romain for assistance with measurements. We are also indebted to the employees at the Wind River Canopy Crane Research Facility, particularly M. Schroeder. M. McCord, K. Anlauf, R. Johnson, and K. Kirchner were helpful with sample collection, and S. Voelker and M. Huso provided very useful discussions on statistics. NR 49 TC 23 Z9 23 U1 2 U2 33 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD JUN PY 2011 VL 98 IS 6 BP 1007 EP 1015 DI 10.3732/ajb.1000503 PG 9 WC Plant Sciences SC Plant Sciences GA 774OJ UT WOS:000291395600019 PM 21613067 ER PT J AU Nghiem, NP Ramirez, EC McAloon, AJ Yee, W Johnston, DB Hicks, KB AF Nghiem, Nhuan P. Ramirez, Edna C. McAloon, Andrew J. Yee, Winnie Johnston, David B. Hicks, Kevin B. TI Economic analysis of fuel ethanol production from winter hulled barley by the EDGE (Enhanced Dry Grind Enzymatic) process SO BIORESOURCE TECHNOLOGY LA English DT Article DE Barley; Fuel ethanol; Economic modeling; EDGE process ID QUICK FIBER PROCESS; ENGINEERING PROCESS; COST MODEL; CORN AB A process and cost model was developed for fuel ethanol production from winter barley based on the EDGE (Enhanced Dry Grind Enzymatic) process. In this process, in addition to beta-glucanases, which are added to reduce the viscosity of the mash, beta-glucosidase is also added to completely hydrolyze the oligomers obtained during the hydrolysis of beta-glucans to glucose. The model allows determination of capital costs, operating costs, and ethanol production cost for a plant producing 40 million gallons of denatured fuel ethanol annually. A sensitivity study was also performed to examine the effects of beta-glucosidase and barley costs on the final ethanol production cost. The results of this study clearly demonstrate the economic benefit of adding beta-glucosidase. Lower ethanol production cost was obtained compared to that obtained without beta-glucosidase addition in all cases except one where highest beta-glucosidase cost allowance and lowest barley cost were used. Published by Elsevier Ltd. C1 [Nghiem, Nhuan P.; Ramirez, Edna C.; McAloon, Andrew J.; Yee, Winnie; Johnston, David B.; Hicks, Kevin B.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Nghiem, NP (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM John.Nghiem@ars.usda.gov FU Genencor, a Danisco Division [58-3K95-6-1155-M]; ARS-USDA FX This work was partly funded by Cooperative Research and Development Agreement # 58-3K95-6-1155-M between Genencor, a Danisco Division, and ARS-USDA. NR 25 TC 11 Z9 11 U1 2 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUN PY 2011 VL 102 IS 12 BP 6696 EP 6701 DI 10.1016/j.biortech.2011.03.109 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 774IL UT WOS:000291378800009 PM 21511462 ER PT J AU Goerndt, ME Monleon, VJ Temesgen, H AF Goerndt, Michael E. Monleon, Vicente J. Temesgen, Hailemariam TI A comparison of small-area estimation techniques to estimate selected stand attributes using LiDAR-derived auxiliary variables SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID MEAN-SQUARED ERROR; FOREST ATTRIBUTES; PREDICTORS; IMPUTATION; DIAMETER; BIOMASS; TREES AB One of the challenges often faced in forestry is the estimation of forest attributes for smaller areas of interest within a larger population. Small-area estimation (SAE) is a set of techniques well suited to estimation of forest attributes for small areas in which the existing sample size is small and auxiliary information is available. Selected SAE methods were compared for estimating a variety of forest attributes for small areas using ground data and light detection and ranging (LiDAR) derived auxiliary information. The small areas of interest consisted of delineated stands within a larger forested population. Four different estimation methods were compared for predicting forest density (number of trees/ha), quadratic mean diameter (cm), basal area (m(2)/ha), top height (m), and cubic stem volume (m(3)/ha). The precision and bias of the estimation methods (synthetic prediction (SP), multiple linear regression based composite prediction (CP), empirical best linear unbiased prediction (EBLUP) via Fay-Herriot models, and most similar neighbor (MSN) imputation) are documented. For the indirect estimators, MSN was superior to SP in terms of both precision and bias for all attributes. For the composite estimators, EBLUP was generally superior to direct estimation (DE) and CP, with the exception of forest density. C1 [Goerndt, Michael E.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Monleon, Vicente J.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Temesgen, Hailemariam] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. RP Goerndt, ME (reprint author), Univ Missouri, Dept Forestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM goerndtm@missouri.edu FU Forest Inventory and Analysis (FIA), U.S. Department of Agriculture Forest Service FX We are grateful to Emmor Nile and Dave Enck of the Oregon Department of Forestry for providing both LiDAR and ground data for use in this study. We thank Michael Wing for his expertise with regard to GIS. We also thank Greg Latta, Matt Gregory, and Emilie Grossmann for their superb advice regarding mapping, imputation, and many other aspects of this study. Funding for this project was provided by Forest Inventory and Analysis (FIA), U.S. Department of Agriculture Forest Service. NR 36 TC 9 Z9 10 U1 2 U2 8 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2011 VL 41 IS 6 BP 1189 EP 1201 DI 10.1139/X11-033 PG 13 WC Forestry SC Forestry GA 775GR UT WOS:000291447300004 ER PT J AU Kelsey, RG Joseph, G McWilliams, MG AF Kelsey, Rick G. Joseph, Gladwin McWilliams, Michael G. TI Ethanol synthesis by anoxic root segments from five cedar species relates to their habitat attributes but not their known differences in vulnerability to Phytophthora lateralis root disease SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID PORT-ORFORD-CEDAR; MENZIESII MIRB. FRANCO; ZOOSPORE CHEMOTAXIS; SEEDLING GROWTH; PH REGULATION; DOUGLAS-FIR; PACIFIC YEW; TOLERANCE; GERMINATION; METABOLISM AB Ethanol synthesis by anoxic root segments from Port Orford cedar (Chamaecyparis lawsoniana (A. Murray bis) Parl.); yellow cedar (Chamaecyparis nootkatensis (D. Don) Spach); Atlantic white cedar (Chamaecyparis thyoides (L.) Britton, Sterns & Poggenb.); western redcedar (Thuja plicata Donn ex D. Don), and incense cedar (Calocedrus decurrens (Torr.) Florin) was compared to determine whether the amounts that they produced during flooding could contribute the known greater vulnerability of Port Orford cedar to infection by Phytophthora lateralis Tucker & Milbrat. Roots were incubated in water at 5, 15, 25, and 35 degrees C for 14 days with periodic sampling. After 12 h of anoxic stress, Atlantic white cedar and yellow cedar roots produced equal quantities of ethanol that were about two times more than produced by the other three species, which did not differ from one another. The roots remained anoxic for 14 days, with ethanol concentrations increasing 6 to 11 times depending on the species. After 14 days, Atlantic white cedar remained the highest ethanol producer at two to three times more than the other species, whereas incense cedar yields were the lowest. Yellow cedar, western redcedar, and Port Orford cedar had intermediate levels of ethanol. The similarity in responses of Port Orford cedar to the other species is strong evidence that ethanol is not an important contributor to its known greater vulnerability to P. lateralis infection. In general, root incubation temperature affected ethanol synthesis similarly for all species. Increases in temperature from 5 to 15 degrees C or 15 to 25 degrees C doubled the ethanol yields at 12 h. Literature ratings of anaerobic tolerance for these cedars were compared with ratings based on their ethanol yields after 12 h or 14 days of anoxia. The latter rating appears to more closely correspond with the cedars associations to wet, mesic environments and their likelihood of experiencing anoxia via flooding. C1 [Kelsey, Rick G.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Joseph, Gladwin] ATREE, Bangalore 560064, Karnataka, India. [McWilliams, Michael G.] Oregon Dept Forestry, Salem, OR 97310 USA. RP Kelsey, RG (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3200 Jefferson Way, Corvallis, OR 97331 USA. EM rkelsey@fs.fed.us NR 58 TC 2 Z9 2 U1 1 U2 8 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2011 VL 41 IS 6 BP 1202 EP 1211 DI 10.1139/X11-043 PG 10 WC Forestry SC Forestry GA 775GR UT WOS:000291447300005 ER PT J AU Long, RP Horsley, SB Hall, TJ AF Long, Robert P. Horsley, Stephen B. Hall, Thomas J. TI Long-term impact of liming on growth and vigor of northern hardwoods SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID SUGAR MAPLE; ALLEGHENY PLATEAU; FOREST SOILS; CROWN VIGOR; NUTRITION; STRESS; PENNSYLVANIA; STANDS; INDICATORS; MANGANESE AB Sugar maple (Acer saccharum Marsh.) is a keystone species in the northern hardwood forest, and decline episodes have negatively affected the growth and health of sugar maple in portions of its range over the past 50+ years. Crown health, growth, survival, and flower and seed production of sugar maple were negatively affected by a widespread decline event in the mid-1980s on the unglaciated Allegheny Plateau in northern Pennsylvania. A long-term liming study was initiated in 1985 to evaluate responses to a one-time application of 22.4 Mg.ha(-1) of dolomitic limestone in four northern hardwood stands. Over the 23-year period ending in 2008, sugar maple basal area increment (BAINC) increased significantly (P <= 0.05) in limed plots from 1995 through 2008, whereas American beech (Fagus grandifolia Ehrh.) BAINC was unaffected. For black cherry (Prunus serotina Ehrh.), the third principal overstory species, BAINC and survival were reduced in limed plots compared with unlimed plots. Foliar Ca and Mg remained significantly higher in sugar maple foliage sampled 21 years after lime application, showing persistence of the lime effect. These results show long-term species-specific responses to lime application. C1 [Long, Robert P.; Horsley, Stephen B.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. [Hall, Thomas J.] Penn Bur Forestry, Div Forest Pest Management, Middletown, PA 17057 USA. RP Long, RP (reprint author), US Forest Serv, USDA, No Res Stn, POB 267,335 Natl Forge Rd, Irvine, PA 16329 USA. EM rlong@fs.fed.us NR 44 TC 26 Z9 26 U1 5 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2011 VL 41 IS 6 BP 1295 EP 1307 DI 10.1139/X11-049 PG 13 WC Forestry SC Forestry GA 775GR UT WOS:000291447300013 ER PT J AU Bacon, CW Hinton, DM AF Bacon, Charles W. Hinton, Dorothy M. TI In planta reduction of maize seedling stalk lesions by the bacterial endophyte Bacillus mojavensis SO CANADIAN JOURNAL OF MICROBIOLOGY LA English DT Article DE Bacillus mojavensis; bacterial endophyte; biological control; Fusarium verticillioides; fumonisin; fusaric acid ID FUSARIC ACID; FUMONISIN PRODUCTION; GIBBERELLA-FUJIKUROI; BIOLOGICAL-CONTROL; WILT TOXIN; VERTICILLIOIDES; MONILIFORME; CORN; BIOCONTROL; PHYTOTOXICITY AB Maize (Zea mays L.) is susceptible to infection by Fusarium verticillioides through autoinfection and alloinfection, resulting in diseases and contamination of maize kernels with the fumonisin mycotoxins. Attempts at controlling this fungus are currently being done with biocontrol agents such as bacteria, and this includes bacterial endophytes, such as Bacillus mojavensis. In addition to producing fumonisins, which are phytotoxic and mycotoxic, F. verticillioides also produces fusaric acid, which acts both as a phytotoxin and as an antibiotic. The question now is Can B. mojavensis reduce lesion development in maize during the alloinfection process, simulated by internode injection of the fungus? Mutant strains of B. mojavensis that tolerate fusaric acid were used in a growth room study to determine the development of stalk lesions, indicative of maize seedling blight, by co-inoculations with a wild-type strain of F. verticillioides and with non-fusaric acid producing mutants of F. verticillioides. Lesions were measured on 14-day-old maize stalks consisting of treatment groups inoculated with and without mutants and wild-type strains of bacteria and fungi. The results indicate that the fusaric-acid-tolerant B. mojavensis mutant reduced stalk lesions, suggesting an in planta role for this substance as an antibiotic. Further, lesion development occurred in maize infected with F. verticillioides mutants that do not produce fusaric acid, indicating a role for other phytotoxins, such as the fumonisins. Thus, additional pathological components should be examined before strains of B. mojavensis can be identified as being effective as a biocontrol agent, particularly for the control of seedling disease of maize. C1 [Bacon, Charles W.; Hinton, Dorothy M.] ARS, Toxicol & Mycotoxin Res Unit, USDA, Russell Res Ctr, Athens, GA 30604 USA. RP Bacon, CW (reprint author), ARS, Toxicol & Mycotoxin Res Unit, USDA, Russell Res Ctr, POB 5677, Athens, GA 30604 USA. EM charles.bacon@ars.usda.gov NR 47 TC 4 Z9 4 U1 1 U2 17 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0008-4166 J9 CAN J MICROBIOL JI Can. J. Microbiol. PD JUN PY 2011 VL 57 IS 6 BP 485 EP 492 DI 10.1139/W11-031 PG 8 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology GA 775HO UT WOS:000291449800005 PM 21635192 ER PT J AU Locke, MA Weaver, MA Zablotowicz, RM Steinriede, RW Bryson, CT Cullum, RF AF Locke, M. A. Weaver, M. A. Zablotowicz, R. M. Steinriede, R. W. Bryson, C. T. Cullum, R. F. TI Constructed wetlands as a component of the agricultural landscape: Mitigation of herbicides in simulated runoff from upland drainage areas SO CHEMOSPHERE LA English DT Article DE Atrazine; Fluometuron; Water quality; Wetland ID ATRAZINE; DEGRADATION; MINERALIZATION; MANAGEMENT; REMOVAL; SYSTEM; SOIL AB Constructed wetlands are a recommended practice for buffering pollutant source areas and receiving waters. A wetland consisting of a sediment trap and two treatment cells was constructed in a Mississippi Delta lake watershed. A 3-h simulated runoff event was initiated (2003) to evaluate fate and transport of atrazine and fluometuron through the wetland. Water samples were collected during a runoff simulation and then afterward at selected intervals for 21 d, and analyzed for the herbicides. Breakthrough patterns for herbicide concentrations in water samples during the first 20 h after simulated runoff showed peak concentrations in the first 6 h, with gradual tailing as the herbicide pulse was diluted in the second, excavated (deeper) cell. Atrazine and fluometuron concentrations in the first (shallower, non-excavated) cell averaged 12- and 20-fold greater, respectively, than those in the second cell following simulated runoff, indicating entrapment in the first cell. Atrazine and fluometuron concentrations in the shallower cell decreased 32% and 22%, respectively, 9 d following simulated runoff, indicating either degradation or sorption to soil or wetland flora. In the excavated cell, concentrations were even lower, and atrazine declined more rapidly than fluometuron. Results indicate constructed wetlands can improve downstream water quality though sequestration or processing of pollutants. Published by Elsevier Ltd. C1 [Locke, M. A.; Steinriede, R. W.; Cullum, R. F.] ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. [Weaver, M. A.] ARS, USDA, Biol Control Pests Res Unit, Stoneville, MS USA. [Zablotowicz, R. M.; Bryson, C. T.] ARS, USDA, Crop Prod Syst Res Unit, Stoneville, MS USA. RP Locke, MA (reprint author), ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, 598 McElroy Drive, Oxford, MS 38655 USA. EM Martin.Locke@ars.usda.gov OI Zablotowicz, Robert/0000-0001-8070-1998; Weaver, Mark/0000-0001-9020-7264 NR 31 TC 13 Z9 15 U1 1 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 J9 CHEMOSPHERE JI Chemosphere PD JUN PY 2011 VL 83 IS 11 BP 1532 EP 1538 DI 10.1016/j.chemosphere.2011.01.034 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 774JJ UT WOS:000291381200015 PM 21329960 ER PT J AU Baker, LB Stofan, JR Lukaski, HC Horswill, CA AF Baker, Lindsay B. Stofan, John R. Lukaski, Henry C. Horswill, Craig A. TI Exercise-Induced Trace Mineral Element Concentration in Regional Versus Whole-Body Wash-Down Sweat SO INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM LA English DT Article DE sweat composition; sweat calcium concentration; sweat copper concentration; sweat magnesium concentration ID IRON LOSS; LOSSES; HEAT; ZINC; MAGNESIUM; PERFORMANCE; COLLECTION; EXCRETION; CALCIUM; METALS AB Simultaneous whole-body wash-down (WBW) and regional skin surface sweat collections were completed to compare regional patch and WBW sweat calcium (Ca), magnesium (Mg), copper (Cu), manganese (Mn), iron (Fe), and zinc (Zn) concentrations. Athletes (4 men, 4 women) cycled in a plastic open-air chamber for 90 min in the heat. Before exercise, the subjects and cycle ergometer (covered in plastic) were washed with deionized water. After the onset of sweating, sterile patches were attached to the forearm, back, chest, forehead, and thigh and removed on saturation. After exercise, the subjects and cycle ergometer were washed with 5 L of 15-mM ammonium sulfate solution to collect all sweat minerals and determine the volume of unevaporated sweat. Control trials were performed to measure mineral contamination in regional and WBW methods. Because background contamination in the collection system was high for WBW Mn, Fe, and Zn, method comparisons were not made for these minerals. After correction for minimal background contamination, WBW sweat [Ca], [Mg], and [Cu] were 44.6 +/- 20.0, 9.8 +/- 4.8, and 0.125 +/- 0.069 mg/L, respectively, and 5-site regional (weighted for local sweat rate and body surface area) sweat [Ca], [Mg], and [Cu] were 59.0 +/- 15.9, 14.5 +/- 4.8, and 0.166 +/- 0.031 mg/L, respectively. Five-site regional [Ca], [Mg], and [Cu] overestimated WBW by 32%, 48%, and 33%, respectively. No individual regional patch site or 5-site regional was significantly correlated with WBW sweat [Ca] (r = -.21, p = .65), [Mg] (r = .49, p = .33), or [Cu] (r = .17, p = .74). In conclusion, regional sweat [Ca], [Mg], and [Cu] are not accurate surrogates for or significantly correlated with WBW sweat composition. C1 [Baker, Lindsay B.; Stofan, John R.; Horswill, Craig A.] Gatorade Sports Sci Inst, Barrington, IL USA. [Lukaski, Henry C.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Baker, LB (reprint author), Gatorade Sports Sci Inst, Barrington, IL USA. FU Gatorade Sports Science Institute; USDA, ARS Grand Forks Human Nutrition Research Center FX This study was funded by the Gatorade Sports Science Institute and the USDA, ARS Grand Forks Human Nutrition Research Center. Authors Lindsay B. Baker and John R. Stofan are employees of the Gatorade Sports Science Institute. NR 39 TC 5 Z9 6 U1 1 U2 7 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1526-484X J9 INT J SPORT NUTR EXE JI Int. J. Sport Nutr. Exerc. Metab. PD JUN PY 2011 VL 21 IS 3 BP 233 EP 239 PG 7 WC Nutrition & Dietetics; Sport Sciences SC Nutrition & Dietetics; Sport Sciences GA 776UV UT WOS:000291564900007 PM 21719904 ER PT J AU Ely, MR Kenefick, RW Cheuvront, SN Chinevere, TD Lacher, CP Lukaski, HC Montain, SJ AF Ely, Matthew R. Kenefick, Robert W. Cheuvront, Samuel N. Chinevere, Troy D. Lacher, Craig P. Lukaski, Henry C. Montain, Scott J. TI Surface contamination artificially elevates initial sweat mineral concentrations SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE zinc; iron; copper; calcium; electrolytes ID EXERCISE-HEAT STRESS; WHOLE-BODY; ZINC CONCENTRATIONS; PROLONGED EXERCISE; TRACE-ELEMENTS; LOSSES; IRON; COLLECTION; COPPER; SKIN AB Several sweat mineral element concentrations decline with serial sampling. Possible causes include reduced dermal mineral concentrations or flushing of surface contamination. The purpose of this study was to simultaneously sample mineral concentrations in transdermal fluid (TDF), sweat, and serum during extended exercise-heat stress to determine if these compartments show the same serial changes during repeat sampling. Sixteen heat-acclimated individuals walked on a treadmill (1.56 m/s, 3.0% grade) in a 35 degrees C, 20% relative humidity (RH), 1 m/s wind environment 50 min each hour for 3 h. Mineral concentrations of Ca, Cu, Fe, K, Mg, Na, and Zn were measured each hour from serum, sweat from upper back (sweat pouch) and arm (bag), and TDF from the upper back. Sites were meticulously cleaned to minimize surface contamination. Mineral concentrations were determined by spectrometry. TDF remained stable over time, with exception of a modest increase in TDF [Fe] (15%) and decrease in TDF [Zn] (-18%). Likewise, serum and pouch sweat samples were stable over time. In contrast, the initial arm bag sweat mineral concentrations were greater than those in the sweat pouch, and [Ca], [Cu], [Mg], and [Zn] declined 26-76% from initial to the subsequent samples, becoming similar to sweat pouch. Nominal TDF mineral shifts do not affect sweat mineral concentrations. Arm bag sweat mineral concentrations are initially elevated due to skin surface contaminants that are not removed despite meticulous cleaning (e. g., under fingernails, on arm hair), then decrease with extended sweating and approach those measured from the scapular region. C1 [Ely, Matthew R.; Kenefick, Robert W.; Cheuvront, Samuel N.; Chinevere, Troy D.; Montain, Scott J.] USA, Environm Med Res Inst, Natick, MA 01760 USA. [Lacher, Craig P.; Lukaski, Henry C.] USDA, Human Nutr Res Ctr, Grand Forks, ND USA. RP Ely, MR (reprint author), USA, Environm Med Res Inst, Kansas St,Bldg 42, Natick, MA 01760 USA. EM matthew.ely@us.army.mil OI Ely, Matthew/0000-0002-0618-7078 NR 41 TC 5 Z9 5 U1 2 U2 6 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JUN PY 2011 VL 110 IS 6 BP 1534 EP 1540 DI 10.1152/japplphysiol.01437.2010 PG 7 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA 776KG UT WOS:000291532600007 PM 21512152 ER PT J AU Dugan, FM Wiest, A McCluskey, K AF Dugan, Frank M. Wiest, Aric McCluskey, Kevin TI Public germplasm collections and revolutions in biotechnology SO JOURNAL OF BIOSCIENCES LA English DT Editorial Material DE Antibiotics; endophyte; ex situ germplasm repository; green revolution; perennial crops; polymerase chain reaction ID POLYMERASE CHAIN-REACTION; DNA C1 [Wiest, Aric; McCluskey, Kevin] Univ Missouri, Fungal Genet Stock Ctr, Kansas City, MO 64110 USA. [Wiest, Aric; McCluskey, Kevin] Univ Missouri, Sch Biol Sci, Kansas City, MO USA. [Dugan, Frank M.] Washington State Univ, USDA ARS, Western Reg Plant Intro & Testing Res Stn, Pullman, WA 99164 USA. RP McCluskey, K (reprint author), Univ Missouri, Fungal Genet Stock Ctr, Kansas City, MO 64110 USA. EM mccluskeyk@umkc.edu NR 24 TC 2 Z9 2 U1 2 U2 3 PU INDIAN ACAD SCIENCES PI BANGALORE PA C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA SN 0250-5991 J9 J BIOSCIENCES JI J. Biosci. PD JUN PY 2011 VL 36 IS 2 BP 205 EP 209 DI 10.1007/s12038-011-9060-y PG 5 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 777ZQ UT WOS:000291665200002 PM 21654073 ER PT J AU Klarica, J Bittner, L Pallua, J Pezzei, C Huck-Pezzei, V Dowell, F Schied, J Bonn, G Huck, C Schlick-Steiner, BC Steiner, FM AF Klarica, Jasmin Bittner, Lukas Pallua, Johannes Pezzei, Christine Huck-Pezzei, Verena Dowell, Floyd Schied, Johannes Bonn, Guenther K. Huck, Christian Schlick-Steiner, Birgit C. Steiner, Florian M. TI Near-Infrared Imaging Spectroscopy as a Tool to Discriminate Two Cryptic Tetramorium Ant Species SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Cuticular hydrocarbons; Ant worker; Infrared spectroscopy; Chemical fingerprinting ID HYMENOPTERA; FORMICIDAE AB Correct species identification is a precondition for many ecological studies. Morphologically highly similar, i.e., cryptic, species are an important component of biodiversity but particularly difficult to discriminate and therefore understudied ecologically. To find new methods for their rapid identification, thus, is important. The cuticle's chemical signature of insects often is unique for species. Near-infrared spectroscopy (NIRS) can capture such signatures. Imaging NIRS facilitates precise positioning of the measurement area on biological objects and high-resolution spatial capturing. Here, we tested the applicability of imaging NIRS to the discrimination of cryptic species by using the ants Tetramorium caespitum and T. impurum. The classification success of Partial Least Squares Regression was 98.8%. Principal Component Analysis grouped spectra of some T. impurum individuals with T. caespitum. Combined with molecular-genetic and morphological evidence, this result enabled us to pose testable hypotheses about the biology of these species. We conclude that discrimination of T. caespitum and T. impurum with imaging NIRS is possible, promising that imaging NIRS could become a time- and cost-efficient tool for the reliable discrimination of cryptic species. This and the direct facilitation of potential biological insight beyond species identification underscore the value of imaging NIRS to ecology. C1 [Klarica, Jasmin; Schied, Johannes; Schlick-Steiner, Birgit C.; Steiner, Florian M.] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Bittner, Lukas; Pallua, Johannes; Pezzei, Christine; Huck-Pezzei, Verena; Bonn, Guenther K.; Huck, Christian] Univ Innsbruck, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria. [Dowell, Floyd] ARS, USDA, Manhattan, KS 66502 USA. RP Klarica, J (reprint author), Univ Innsbruck, Inst Ecol, Technikerstr 25, A-6020 Innsbruck, Austria. EM jasmin.klarica@gmail.com OI Huck, Christian/0000-0002-6272-3242 FU Austrian Science Fund FWF [P 23409] NR 10 TC 10 Z9 11 U1 1 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2011 VL 37 IS 6 BP 549 EP 552 DI 10.1007/s10886-011-9956-x PG 4 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 775UX UT WOS:000291490000001 PM 21537901 ER PT J AU Jang, EB Khrimian, A Siderhurst, MS AF Jang, Eric B. Khrimian, Ashot Siderhurst, Matthew S. TI Di- and Tri-fluorinated Analogs of Methyl Eugenol: Attraction to and Metabolism in the Oriental Fruit Fly, Bactrocera Dorsalis (Hendel) SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Oriental fruit fly. Bactrocera dorsalis (Hendel); 1-(3,3-Difluoroprop-2-en-1-yl)-2-fluoro-4, 5-dimethoxybenzene 1-Fluoro-2-(3-fluoroprop-2-en-1-yl)-4,5-dimethoxybenzene; Methyl eugenol; Metabolism inhibition; Field attraction; Bioassay; Male annihilation ID DACUS-DORSALIS; MONOFLUORO ANALOGS; MALE ANNIHILATION; TEPHRITIDAE; ERADICATION; DIPTERA; SAFROLE; FLIES; POLLINATION; ESTRAGOLE AB Oriental fruit fly, Bactrocera dorsalis (Hendel), males are attracted to the natural phenylpropanoid methyl eugenol (ME). They feed compulsively on ME and metabolize it to ring and side-chain hydroxylated compounds that have both pheromonal and allomonal properties. Previously, we demonstrated that mono-fluorination at the terminal carbon of the ME side-chain significantly reduced metabolic side-chain hydroxylation, while mono-fluorination of ME at position 4 of the aromatic ring blocked ring-hydroxylation but surprisingly enhanced side-chain hydroxylation. Here, we demonstrated that the introduction of fluorine atoms on both the ring and side-chain of ME blocks both positions that undergo enzymatic hydroxylation and, in particular, completely inhibits oxidative biotransformation of the allyl group. In laboratory experiments, B. dorsalis males initially were more attracted to both 1-fluoro-4,5-dimethoxy-2-(3,3-difluoro-2-propenyl)benzene (I) and 1-fluoro-4,5-dimethoxy-2-(3-fluoro-2-propenyl)benzene (II) than to ME. However, both I and II were taken up by flies at rates significantly less than that of ME. Flies fed with difluoroanalog II partially metabolized it to 5-fluoro-4-(3-fluoroprop-2-en-1-yl)-2-methoxyphenol (III), and flies fed with trifluoroanalog I produced 4-(3,3-difluoroprop-2-en-1-yl)-5-fluoro-2-methoxyphenol (V), but the rates of metabolism relative to rates of intakes were much lower compared to those of ME. Flies that consumed either the tri- or difluorinated analog showed higher post-feeding mortality than those that fed on methyl eugenol. In field trials, trifluoroanalog I was similar to 90% less attractive to male B. dorsalis than ME, while difluoroanalog II was similar to 50% less attractive. These results suggest that increasing fluorination can contribute to fly mortality, but the trade off with attractancy makes it unlikely that either a di or trifluorinated ME would be an improvement over ME for detection and/or eradication of this species. C1 [Siderhurst, Matthew S.] Eastern Mennonite Univ, Dept Chem, Harrisonburg, VA 22802 USA. [Jang, Eric B.; Siderhurst, Matthew S.] ARS, US Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. [Khrimian, Ashot] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. RP Siderhurst, MS (reprint author), Eastern Mennonite Univ, Dept Chem, 1200 Pk Rd, Harrisonburg, VA 22802 USA. EM ms826@emu.edu NR 28 TC 2 Z9 2 U1 3 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2011 VL 37 IS 6 BP 553 EP 564 DI 10.1007/s10886-011-9963-y PG 12 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 775UX UT WOS:000291490000002 PM 21614534 ER PT J AU Wallis, CM Huber, DPW Lewis, KJ AF Wallis, Christopher M. Huber, Dezene P. W. Lewis, Kathy J. TI Ecosystem, Location, and Climate Effects on Foliar Secondary Metabolites of Lodgepole Pine Populations from Central British Columbia SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Climate change; Gas chromatography-mass spectrometry (GC-MS); Lignin; Lodgepole pine; Monoterpenoid; Phenolics; Pinus contorta latifolia; Sesquiterpenoid; Diterpenoid ID DOTHISTROMA NEEDLE BLIGHT; BARK BEETLES; FOREST; PLANTS; MANAGEMENT; RESPONSES; LATITUDE; DEFENSE; DISEASE; CANADA AB Lodgepole pines, Pinus contorta Douglas ex Louden var. latifolia Engelm. ex S. Watson, are encountering increased abiotic stress and pest activity due to recent increases in temperature and changes in precipitation throughout their range. This tree species counters these threats by producing secondary metabolites, including phenolics and terpenoids. We examined foliar levels of lignin, soluble phenolics, monoterpenoids, sesquiterpenoids, and diterpenoids in 12 stands in British Columbia, Canada. We used these data to assess associations among foliar secondary metabolite levels and ecosystem, geographic, and climatic variables. Regressions were also performed to observe which combinations of variables best explained secondary metabolite variance. Stands of P. c. latifolia in the Coastal Western Hemlock and Interior Cedar/Hemlock biogeoclimatic zones had consistently greater foliar levels of almost all measured secondary metabolites than did other stands. Lignin was present in greater amounts in Boreal White/Black Spruce ecosystem (i.e., northern) stands than in southern stands, suggesting a role for this metabolite in pine survival in the boreal forest. Attempts to develop regression models with geographic and climatic variables to explain foliar secondary metabolite levels resulted in multiple models with similar predictive capability. Since foliar secondary metabolite levels appeared to vary most between stand ecosystem types and not as much due to geographic and climatic variables, metabolic profiles appeared best matched to the stress levels within local environments. It is unknown if differences in secondary metabolite levels are the result of genetic adaptation or phenotypic plasticity, but results from this and other studies suggest that both are important. These results are interpreted in light of ongoing efforts to assist in the migration of certain populations of P. c. latifolia northward in an effort to counter predicted effects of climate change. C1 [Wallis, Christopher M.; Huber, Dezene P. W.; Lewis, Kathy J.] Univ No British Columbia, Ecosyst Sci & Management Program, Prince George, BC V2N 4Z9, Canada. RP Wallis, CM (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM christopher.wallis@ars.usda.gov RI Wallis, Christopher/E-6432-2011; OI Huber, Dezene/0000-0002-6495-1759 FU University of Northern British Columbia; Forest Genetic Council of British Columbia; British Columbia Ministry of Forests and Range; Natural Science and Engineering Research Council of Canada; Canada Research Chairs Program; Canada Foundation for Innovation; British Columbia Knowledge Development Fund FX The authors thank Nick Ukraintz, Alex Woods, Brian Aukema, Sam Barnes, and Rhiannon Montgomery for planning and technical help in executing this study. We also acknowledge the University of Northern British Columbia, the Forest Genetic Council of British Columbia, the British Columbia Ministry of Forests and Range, the Natural Science and Engineering Research Council of Canada, the Canada Research Chairs Program, the Canada Foundation for Innovation, and the British Columbia Knowledge Development Fund for funding and other support in conducting this research. NR 45 TC 9 Z9 11 U1 1 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2011 VL 37 IS 6 BP 607 EP 621 DI 10.1007/s10886-011-9958-8 PG 15 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 775UX UT WOS:000291490000008 PM 21537900 ER PT J AU Childers, CC Haley, E Jahns, L AF Childers, Courtney Carpenter Haley, Eric Jahns, Lisa TI Insights into University Freshman Weight Issues and How They Make Decisions About Eating SO JOURNAL OF CONSUMER AFFAIRS LA English DT Article ID VIGOROUS PHYSICAL-ACTIVITY; COLLEGE-STUDENTS; BODY-WEIGHT; EXERCISE; BEHAVIOR; OBESITY; TRANSITION; HEALTH; GAIN; WOMEN AB The transition from high school to college represents a life turning point during which health behavior paths may be influenced. This study addresses the internal and external factors that guide students' eating decisions as they are understood and relayed by students through qualitative methods. A sample of 102 second semester college freshmen participated in a two-week data collection period. Results show that many students are caught in a negative cycle of food choices with notable social and environmental determinants impacting both their physical and emotional health. C1 [Childers, Courtney Carpenter; Haley, Eric] Univ Tennessee, Knoxville, TN 37996 USA. [Jahns, Lisa] USDA, Washington, DC USA. RP Childers, CC (reprint author), Univ Tennessee, Knoxville, TN 37996 USA. EM childers@utk.edu; haley@utk.edu; Lisa.Jahns@ars.usda.gov OI Jahns, Lisa/0000-0002-1828-6962 NR 39 TC 5 Z9 5 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0078 EI 1745-6606 J9 J CONSUM AFF JI J. Consum. Aff. PD SUM PY 2011 VL 45 IS 2 BP 306 EP 328 DI 10.1111/j.1745-6606.2011.01204.x PG 23 WC Business; Economics SC Business & Economics GA 773AA UT WOS:000291278200007 ER PT J AU Gurtler, JB Marks, HM Jones, DR Bailey, RR Bauer, NE AF Gurtler, Joshua B. Marks, Harry M. Jones, Deana R. Bailey, Rebecca R. Bauer, Nathan E. TI Modeling the Thermal Inactivation Kinetics of Heat-Resistant Salmonella Enteritidis and Oranienburg in 10 Percent Salted Liquid Egg Yolk SO JOURNAL OF FOOD PROTECTION LA English DT Article ID LISTERIA-MONOCYTOGENES; PRODUCTS; DESTRUCTION; TEMPERATURES; PASTEURIZATION; SEROVARS; CURVES; SUGAR AB There is no suitable model for predicting thermal inactivation kinetics of Salmonella spp. for many types of liquid egg products, including salted liquid egg yolk, for use in updating U.S. Department of Agriculture (USDA) pasteurization guidelines. This is because, in part, of the variations in Salmonella strains and the changes in the processing of liquid egg products over the past 40 years. The objectives of the present study were to determine the thermal inactivation kinetics and to create a general thermal inactivation kinetics model that can be used for estimating log reductions of salmonellae in 10% salted liquid egg yolk for temperatures between 62.2 and 69 degrees C. This model can be used by processors to help ensure adequate pasteurization. This was accomplished by studying the inactivation kinetics of a three-strain composite of heat-resistant Salmonella serovars Enteritidis and Oranienburg, inoculated into commercially processed 10% salted liquid egg yolk. The survival curves were convex, with asymptotic D-values. From these curves, a general model was developed to predict log reductions for given times at specified temperatures. For example, at a temperature of 67.3 degrees C (153.1 degrees F) for 3.5 min, our model predicts a 5-log reduction would be obtained, whereas with the current USDA minimum required pasteurization regimen (63.33 degrees C [146 degrees F] for 3.5 min), our model predicts that a reduction of only 2.7 log would be obtained. The results of this study provide information that can be used by processors to aid in producing safe, pasteurized egg yolk products, and for satisfying USDA pasteurization performance standards and developing industry guidance. C1 [Gurtler, Joshua B.; Bailey, Rebecca R.] Agr Res Serv, USDA, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Marks, Harry M.] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Jones, Deana R.] Agr Res Serv, USDA, Egg Safety & Qual Res Unit, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. [Bauer, Nathan E.] US Food Safety & Inspect Serv, USDA, College Stn, TX 77845 USA. RP Gurtler, JB (reprint author), Agr Res Serv, USDA, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Joshua.gurtler@ars.usda.gov FU FSIS, Washington, DC FX This research was funded based on needs prioritized by the FSIS, Washington, DC. The authors would like to thank our internal USDA reviewers: David Geveke, Lihan Huang, Andy Hwang, and Shiowshuh Sheen for proofreading this article and providing helpful feedback. The 10% salted liquid egg yolk that was used in this study, provided by Michael Foods, Gaylord, MN, is very much appreciated. Egg quality assessment technical assistance was provided by Patsy Mason and Victoria Broussard. NR 39 TC 5 Z9 5 U1 1 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2011 VL 74 IS 6 BP 882 EP 892 DI 10.4315/0362-028X.JFP-10-384 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 776HG UT WOS:000291524500003 PM 21669063 ER PT J AU Lu, HYJ Breidt, F Perez-Diaz, IM Osborne, JA AF Lu, Huiying J. Breidt, Frederick, Jr. Perez-Diaz, Ilenys M. Osborne, Jason A. TI Antimicrobial Effects of Weak Acids on the Survival of Escherichia coli O157:H7 under Anaerobic Conditions SO JOURNAL OF FOOD PROTECTION LA English DT Article ID HEMOLYTIC-UREMIC SYNDROME; UNITED-STATES; FUMARIC-ACID; APPLE CIDER; ALTERNATIVE METHOD; 5-LOG REDUCTION; SODIUM BENZOATE; SORBIC ACID; O157-H7; PH AB Outbreaks of disease due to vegetative bacterial pathogens associated with acid foods (such as apple cider) have raised concerns about acidified vegetables and related products that have a similar pH (3.2 to 4.0). Escherichia coli O157:H7 and related strains of enterohemorrhagic E. call (EHEC) have been identified as the most acid resistant vegetative pathogens in these products. Previous research has shown that the lack of dissolved oxygen in many hermetically sealed acid or acidified food products can enhance survival of EHEC compared with their survival under aerobic conditions. We compared the antimicrobial effects of several food acids (acetic, malic, lactic, fumaric, benzoic, and sorbic acids and sulfite) on a cocktail of EHEC strains under conditions representative of non heat-processed acidified vegetables in hermetically sealed jars, holding the pH (3.2) and ionic strength (0.342) constant under anaerobic conditions. The overall antimicrobial effectiveness of weak acids used in this study was ranked, from most effective to least effective: sulfite > benzoic acid > sorbic acid > fumaric acid > L- and o-lactic acid > acetic acid > malic acid. These rankings were based on the estimated protonated concentrations required to achieve a Slog reduction in EHEC after 24 h of incubation at 30 degrees C. This study provides information that can be used to formulate safer acid and acidified food products and provides insights about the mode of action of weak acids against EHEC. C1 [Breidt, Frederick, Jr.; Perez-Diaz, Ilenys M.] N Carolina State Univ, USDA, S Atlantic Area, Food Sci Res Unit,Agr Res Serv, Raleigh, NC 27695 USA. [Lu, Huiying J.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Osborne, Jason A.] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. RP Breidt, F (reprint author), N Carolina State Univ, USDA, S Atlantic Area, Food Sci Res Unit,Agr Res Serv, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM fred.breidt@ars.usda.gov NR 47 TC 6 Z9 6 U1 3 U2 9 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2011 VL 74 IS 6 BP 893 EP 898 DI 10.4315/0362-028X.JFP-10-404 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 776HG UT WOS:000291524500004 PM 21669064 ER PT J AU Niemira, BA AF Niemira, Brendan A. TI Influence of Refrigerated Storage Time on Efficacy of Irradiation To Reduce Salmonella on Sliced Roma Tomatoes SO JOURNAL OF FOOD PROTECTION LA English DT Article ID 1-PERCENT CALCIUM-CHLORIDE; GAMMA-IRRADIATION; DICED TOMATOES; LISTERIA-MONOCYTOGENES; FRESH PRODUCE; LETTUCE; ENTERICA; SPINACH; LEAVES AB Contamination of tomatoes with Salmonella is a recurring food safety concern. Irradiation is a nonthermal intervention that can inactivate pathogens on fresh and minimally processed produce. However, the influence of tomato processing protocols, including time in refrigerated storage and time between slicing and irradiation, has not been determined. Roma tomatoes were sliced and inoculated with a cocktail of Salmonella outbreak strains. The inoculated tomatoes were held in refrigerated storage for various times after inoculation to simulate the potential time delay between packaging and irradiation. Tomatoes were irradiated immediately (0 h) or after 24, 48, or 72 h in storage. The surviving populations were recovered and enumerated. Irradiation effectively reduced Salmonella at all times. The D(10)-values (the dose necessary for a 1-log reduction of the pathogen) were not significantly different at each storage time and ranged from 0.382 to 0.473 kGy. These results suggest that the time required for holding of processed Roma tomatoes or shipment to an off-site irradiation service provider will not alter the efficacy of irradiation in a commercial environment. C1 Agr Res Serv, USDA, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Niemira, BA (reprint author), Agr Res Serv, USDA, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM brendan.niemira@ars.usda.gov NR 16 TC 1 Z9 1 U1 1 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2011 VL 74 IS 6 BP 990 EP 993 DI 10.4315/0362-028X.JFP-10-385 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 776HG UT WOS:000291524500018 PM 21669078 ER PT J AU Gould, LH Seys, S Everstine, K Norton, D Ripley, D Reimann, D Dreyfuss, M Chen, WS Selman, CA AF Gould, L. Hannah Seys, Scott Everstine, Karen Norton, Dawn Ripley, Danny Reimann, David Dreyfuss, Moshe Chen, Wu San Selman, Carol A. TI Recordkeeping Practices of Beef Grinding Activities at Retail Establishments SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI; GROUND-BEEF AB Ground beef has been implicated as a transmission vehicle in foodborne outbreaks of infection with pathogens such as Escherichia coli O157:H7 and Salmonella. During outbreak investigations, traceback of contaminated beef to the producing facility is often unsuccessful because of inadequate recordkeeping at retail establishments that grind beef products. We conducted a survey in three states participating in the Environmental Health Specialists Network to describe beef grinding and recordkeeping practices at retail establishments. In each establishment that maintained grinding logs, three randomly selected records were reviewed to determine whether important data elements for traceback investigations were recorded. One hundred twenty-five stores were surveyed, of which 60 (49%) kept grinding logs, including 54 (74%) of 73 chain stores and 6 (12%) of 51 independent stores. One hundred seventy-six grinding records from 61 stores were reviewed. Seventy-three percent of the records included the establishment code of the source beef, 72% included the grind date and time, and 59% included the lot number of the source beef. Seventy-five percent of records noted whether trimmings were included in grinds, and 57% documented cleanup activities. Only 39 (22%) records had all of these variables completed. Of stores that did not keep grinding logs, 40% were unaware of their purpose. To facilitate effective and efficient traceback investigations by regulatory agencies, retail establishments should maintain records more detailed and complete of all grinding activities. C1 [Gould, L. Hannah] Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Seys, Scott] US Food Safety & Inspect Serv, Foodborne Dis Invest Branch, USDA, Washington, DC 20250 USA. [Everstine, Karen; Reimann, David] Minnesota Dept Hlth, St Paul, MN 55101 USA. [Norton, Dawn] Calif Emerging Infect Program, Oakland, CA 94612 USA. [Ripley, Danny] Nashville Davidson Cty Metro Publ Hlth Dept, Nashville, TN 37201 USA. [Dreyfuss, Moshe; Chen, Wu San] US Food Safety & Inspect Serv, Microbiol Issues Branch, USDA, Aerosp Ctr, Washington, DC 20250 USA. [Selman, Carol A.] Ctr Dis Control & Prevent, Environm Hlth Serv Branch, Natl Ctr Environm Hlth, Atlanta, GA 30341 USA. RP Gould, LH (reprint author), Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Natl Ctr Emerging & Zoonot Infect Dis, 1600 Clifton Rd NE,MS D63, Atlanta, GA 30333 USA. EM lgould@cdc.gov NR 10 TC 3 Z9 3 U1 0 U2 2 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2011 VL 74 IS 6 BP 1022 EP 1024 DI 10.4315/0362-028X.JFP-10-370 PG 3 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 776HG UT WOS:000291524500025 PM 21669085 ER PT J AU Dainat, B Evans, JD Chen, YP Neumann, P AF Dainat, Benjamin Evans, Jay D. Chen, Yan Ping Neumann, Peter TI Sampling and RNA quality for diagnosis of honey bee viruses using quantitative PCR SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Apis mellifera; Viruses; Diagnosis; Sampling; Conservation; RNA ID RT-PCR; APIS-MELLIFERA AB Molecular diagnoses of pathogens via ribonucleic acid (RNA) signatures are used widely in honey bee pathology. Such diagnoses can be compromised by ubiquitous and endogenous RNA-degrading enzymes activated after the death of sampled bees. RNA degradation can be minimized by storage at ultra-cold temperatures or by immersion in high-salt buffers. However, these methods are not always available in the field or are costly, driving a search for alternative methods to store and transport bees for RNA analyses. While the impact of storage conditions on RNA integrity has been evaluated, the tolerance of standard RT-qPCR diagnostic methods of honey bee pathogens for suboptimal collection and storage is unknown. Given the short regions of RNA used for pathogen diagnosis (generally amplified regions of 100-200 nucleotides), it is conceivable that even degraded RNA will provide a template for precise diagnosis. In this study, the impact of the two most convenient sample storage and handling methods (+4 degrees C and ambient temperature) for identifying honey bee virus infections was evaluated by RT-qPCR The aim was to streamline the methods needed to collect, transport, and store honey bee samples destined for pathogen diagnosis. The data show that samples held at room temperature for times anticipated for sample transport for up to 5 days are suitable for diagnosis of two of the most common and prevalent honey bee viruses, deformed wing virus (DWV) and black queen cell virus (BQCV). The results will be useful for the standardisation of sampling methods across countries and laboratories. (C) 2011 Elsevier B.V. All rights reserved. C1 [Dainat, Benjamin; Neumann, Peter] Agroscope Liebefeld Posieux Res Stn ALP, Swiss Bee Res Ctr, CH-3003 Bern, Switzerland. [Evans, Jay D.; Chen, Yan Ping] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Neumann, Peter] Rhodes Univ, Dept Zool & Entomol, ZA-61440 Grahamstown, South Africa. RP Dainat, B (reprint author), Agroscope Liebefeld Posieux Res Stn ALP, Swiss Bee Res Ctr, CH-3003 Bern, Switzerland. EM benjamin.dainat@alp.admin.ch RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU Swiss Federal Veterinary Office FX We would like to thank Dawn Lopez and Michelle Hamilton for their valuable laboratory assistance and Andrew Ulsamer for the beekeeping assistance. Appreciation is also addressed to two anonymous referees for constructive comments, to Ueli Buetikofer and Tioka Rabeony for statistical advice. We are grateful to A. Zuckerman for helpful editorial comments. Financial support was granted by the Swiss Federal Veterinary Office to PN and BD. Use of commercial names in this paper is for information only. There are no conflicts of interest to be declared. NR 10 TC 10 Z9 10 U1 1 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 J9 J VIROL METHODS JI J. Virol. Methods PD JUN PY 2011 VL 174 IS 1-2 BP 150 EP 152 DI 10.1016/j.jviromet.2011.03.029 PG 3 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA 776FX UT WOS:000291521000025 PM 21473885 ER PT J AU Somogyi, A Hathcock, J Biesalski, HK Blumberg, JB Antoine, JM Edwards, G Prock, P AF Somogyi, Arpad Hathcock, John Biesalski, Hans Konrad Blumberg, Jeffrey B. Antoine, Jean-Michel Edwards, Gareth Prock, Peter TI Scientific issues related to Codex Alimentarius goals: A review of principles, with examples SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Codex; Codex Alimentarius; Nutrients; Supplements; Health claims; Benefits; Risk assessment; Safety ID FOODS; BSE AB The Codex Alimentarius provides the food standards and guidelines recognized by the World Trade Organization as the primary authority for use in settlement of related trade disputes. Codex bases its decisions primarily on scientific principles and evidence, although other legitimate factors such as economic and societal values may be considered. Codex has two primary aims: to protect consumers' health and assure fair practices in food trade. Codex documents may provide templates for individual nations but are not binding for domestic policies. Despite many advances over the last couple of decades, misunderstandings and controversies have interfered with important aspects of progress which Codex needs to accomplish, especially in the areas of claims of benefits related to food or nutrient consumption and the establishment of the safety of these items. Claims for health benefits should be based on the totality of available scientific evidence, including observational data collected from large populations as well as the results from randomized clinical trials. Safety should be evaluated by risk assessment on high quality experimental data, with anecdotal information having a lesser role. Regulatory policy would be improved if "history of safe use" were to be better defined and described. (C) 2011 Elsevier Inc. All rights reserved. C1 [Hathcock, John] Council Responsible Nutr Int, Washington, DC 20036 USA. [Somogyi, Arpad] European Commiss, D-12309 Berlin, Germany. [Biesalski, Hans Konrad] Univ Hohenheim, Dept Biol Chem & Nutr, D-70593 Stuttgart, Germany. [Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Antoine, Jean-Michel] Danone Res, F-91767 Palaiseau, France. [Edwards, Gareth] Gareth Edwards Consultancy, Reading RG4 9TD, Berks, England. [Prock, Peter] European Nutraceut Assoc, CH-4010 Basel, Switzerland. RP Hathcock, J (reprint author), Council Responsible Nutr Int, 1828 L St NW,Suite 510, Washington, DC 20036 USA. EM Jhathcock@crnusa.org NR 25 TC 9 Z9 9 U1 0 U2 3 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD JUN PY 2011 VL 60 IS 1 BP 161 EP 164 DI 10.1016/j.yrtph.2011.02.011 PG 4 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA 774HQ UT WOS:000291376700016 PM 21382429 ER PT J AU Rypel, AL AF Rypel, A. L. TI RIVER IMPOUNDMENT AND SUNFISH GROWTH SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE Centrarchidae; climate; dams; fisheries management; natural flow regime; production; restoration; reservoir retention time ID AQUATIC ECOSYSTEM FRAGMENTATION; FLOOD-PULSE CONCEPT; HYDROLOGIC CONNECTIVITY; FISH ASSEMBLAGES; LARGEMOUTH BASS; ECOLOGICAL INTEGRITY; MISSISSIPPI RIVER; REGULATED RIVERS; BLUEGILL SUNFISH; TIDAL CREEKS AB Impoundment of rivers by dams is widespread and one of the most devastating anthropogenic impacts to freshwater environments. Linking theoretical and applied research on river impoundment requires an improved capacity for predicting how varying degrees of impoundment affects a range of species. Here, growth of 14 North American sunfish species resilient to river impoundment was compared in rivers versus impoundments. Growth response to river impoundment varied widely, but consistently among taxa: five species (shadow bass, rock bass, flier, redbreast sunfish and green sunfish) showed significantly higher growth in riverine ecosystems, four species (largemouth bass, smallmouth bass, spotted bass and longear sunfish) showed significantly higher growth in impounded ecosystems, and five species (bluegill, black crappie, white crappie, redear sunfish and warmouth) displayed no difference in growth between rivers and impoundments. Furthermore, significant linear models were developed for predicting growth of two species (largemouth bass, R-2 = 0.75 and warmouth, R-2 = 0.44) based on a physiographically specific index of reservoir retention time. For another species (white crappie), growth could not be predicted by the retention time index in Central Lowlands rivers (R-2 = 0.001), but was strongly predicted by this factor in southeastern Coastal Plain rivers (R-2 = 0.76) showing how impacts of impoundment, and prediction of its consequences, can vary across river landscape types. Further analysis of fish growth in response to river impoundment, regulation and fragmentation could greatly enhance conservation biology, restoration ecology and basic land use decisions in riverine landscapes. Copyright (C) 2010 John Wiley & Sons, Ltd. C1 Univ Mississippi, Dept Biol, USDA Forest Serv Stream Hydrol Lab, Oxford, MS 38655 USA. RP Rypel, AL (reprint author), Univ Mississippi, Dept Biol, USDA Forest Serv Stream Hydrol Lab, 1000 Front St, Oxford, MS 38655 USA. EM andrewrypel@yahoo.com FU USDA Forest Service FX This research was supported by a USDA Forest Service Post Doctoral Fellowship to A.L.R.I gratefully thank William L. Fisher, Donald A. Jackson, Craig A. Layman and two anonymous reviewers who provided comments on earlier versions of this manuscript. I also thank the following people who contributed data and time into this paper - especially D. Abernethy, D. Armstrong, J. Haffner, P. Ekema, K. Weathers, L. Graves, R. Andress, D. Harrison, J. Hakala, T. Barrett, K. Pounds, S. Kone and J. Rypel. NR 91 TC 14 Z9 14 U1 3 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD JUN PY 2011 VL 27 IS 5 BP 580 EP 590 DI 10.1002/rra.1370 PG 11 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 777DK UT WOS:000291595100004 ER PT J AU Spatz, SJ Schat, KA AF Spatz, Stephen J. Schat, Karel A. TI Comparative genomic sequence analysis of the Marek's disease vaccine strain SB-1 SO VIRUS GENES LA English DT Article DE Marek's disease; Gallid herpesvirus type 3; DNA sequence; Vaccine; SB-1 ID AVIAN-LEUKOSIS VIRUS; LONG TERMINAL REPEAT; RETICULOENDOTHELIOSIS VIRUS; FIELD STRAIN; HERPESVIRUS; LTR; DNA; INTEGRATION; INSERTION AB Marek's disease virus (MDV), an oncogenic alphaherpesvirus, induces a rapid onset T-cell lymphoma and demyelinating disease in chickens. Since the 1970s the disease has been controlled through mass vaccination with herpesvirus of turkeys [meleagrid herpesvirus type 1 (MeHV-1)]. Over time this vaccine's efficacy decreased, and in the 1980s a bivalent vaccine consisting of MeHV-1 and a non-oncogenic gallid herpesvirus type 3 (GaHV-3) strain known as SB-1 was introduced. The complete DNA sequence (165,994 bp) of this GaHV-3 strain was determined using 454 pyrosequencing. A total of 524 open reading frames (ORFs) were examined for homology to protein sequences present in GenBank using BLAST (E-values < 0.9). Of the 128 ORF hits, 75 ORFs showed homology to well-characterized alphaherpesviral proteins. Phylogenetically, this strain partitions in its own branch along with the GaHV-3 strain HPRS24 and shows more relatedness to MeHV-1 than gallid herpesvirus type 2 (GaHV-2, Marek's disease virus). When comparing the GaHV-3 ORFs to their homologues in MeHV-1 and GaHV-2, a greater percentage of amino acid similarity was found with homologous ORFs in the genome of SB-1 than with those in the HPRS24 genome. Overall, twice as many of the 75 ORFs within the SB-1 genome showed greater sequence identities and similarities to homologous ORFs in the Marek's disease genome than those within the HPRS24 genome. This paper describes the sequence difference between the two GaHV-3 genomes. Overall 19 ORFs differ in the number of predicted amino acids; of these, eight (U(L)3.5, U(L)5, U(L)9, U(L)28, U(L)30, U(L)36, U(L)37, and U(L)50) encode well-characterized alphaherpesviral proteins A sequence within the unique short region of the SB-1 genome exhibited significant sequence homology to long terminal repeat (LTR) sequences of avian retroviruses. This sequence was only found in the SB-1 genome and not the HPRS24 genome. C1 [Spatz, Stephen J.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Schat, Karel A.] Cornell Univ, Dept Microbiol & Immunol, Coll Vet Med, Ithaca, NY 14853 USA. RP Spatz, SJ (reprint author), ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. EM Stephen.Spatz@ars.usda.gov; kas24@cornell.edu NR 23 TC 12 Z9 12 U1 0 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD JUN PY 2011 VL 42 IS 3 BP 331 EP 338 DI 10.1007/s11262-011-0573-0 PG 8 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 775VX UT WOS:000291492600004 PM 21336949 ER PT J AU Yu, QZ Estevez, CN Roth, JP Hu, HX Zsak, L AF Yu, Qingzhong Estevez, Carlos N. Roth, Jason P. Hu, Haixia Zsak, Laszlo TI Deletion of the M2-2 gene from avian metapneumovirus subgroup C impairs virus replication and immunogenicity in Turkeys SO VIRUS GENES LA English DT Article DE aMPV-C; Reverse genetics; M2-2 gene deletion; Virus replication; Pathogenicity; Immunogenicity ID RESPIRATORY SYNCYTIAL VIRUS; OPEN READING FRAME; RHINOTRACHEITIS VIRUS; SEQUENCE-ANALYSIS; REVERSE GENETICS; ORDER DIFFERENT; SUBTYPE-C; SH-GENE; PROTEIN; PNEUMOVIRUS AB The second matrix (M2) gene of avian metapneumovirus subgroup C (aMPV-C) contains two overlapping open reading frames (ORFs), encoding two putative proteins, M2-1 and M2-2. Both proteins are believed to be involved in viral RNA transcription or replication. To further characterize the function of the M2-2 protein in virus replication, the non-overlapping region of the M2-2 ORF was deleted from an infectious cDNA clone of the aMPV-C strain, and a viable virus was rescued by using reverse genetics technology. The recombinant virus, raMPV-C Delta M2-2, was characterized in vitro and in vivo. In Vero cells, raMPV-C Delta M2-2 replicated slightly less efficiently than the parental virus, 10-fold reduction at 48-h post-infection. The raMPV-C Delta M2-2 virus induced typical cytopathic effects (CPE) that were indistinguishable from those seen with the parental virus infection. In specific-pathogen-free (SPF) turkeys, raMPV-C Delta M2-2 was attenuated and caused no clinical signs of disease. Less than 20% of the inoculated birds shed detectable virus in tracheal tissue during the first 5 days post-infection, and no virus shedding was detected afterward. Forty percent of infected birds produced a weak antibody response at 14 days post-infection. Upon challenge with a virulent aMPV-C strain, more than 80% of the raMPV-C Delta M2-2-inoculated birds showed typical disease signs and virus shedding in tracheal tissue. These results suggest that the M2-2 protein of aMPV-C virus is not essential for virus replication in vitro, but is required for sufficient virus replication to maintain pathogenicity and immunogenicity in the natural host. C1 [Yu, Qingzhong; Estevez, Carlos N.; Roth, Jason P.; Hu, Haixia; Zsak, Laszlo] ARS, USDA, SE Poultry Res Lab, Athens, GA 30605 USA. RP Yu, QZ (reprint author), ARS, USDA, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM qingzhong.yu@ars.usda.gov FU USDA, ARS [6612-32000-056-00D] FX The authors wish to thank Xiuqin Xia and Fenglan Li for excellent technical assistance and Melissa Scott and Joyce Bennett for performing the automated nucleotide sequencing, Darrell Kapczynski for assistant in statistical analysis, and Patti Miller for critical reading of the manuscript. This research was supported by USDA, ARS CRIS project 6612-32000-056-00D. NR 38 TC 3 Z9 4 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD JUN PY 2011 VL 42 IS 3 BP 339 EP 346 DI 10.1007/s11262-011-0577-9 PG 8 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 775VX UT WOS:000291492600005 PM 21287257 ER PT J AU Kim, T Mays, J Fadly, A Silva, RF AF Kim, Taejoong Mays, Jody Fadly, Aly Silva, Robert F. TI Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek's disease virus (MDV) alters expression of nearby MDV genes SO VIRUS GENES LA English DT Article DE Herpesvirusl; Retrovirusl; Poultryl; Recombinationl; LTR, MDV, REV ID AVIAN-LEUKOSIS VIRUS; UNIQUE SHORT REGION; RETROVIRUS INSERTION; IN-VIVO; ESCHERICHIA-COLI; HERPESVIRUS; GENOME; DNA; REPLICATION; INTEGRATION AB Researchers reported that co-cultivating the JM/102W strain of Marek's disease virus (MDV) with reticuloendotheliosis virus (REV) resulted in an REV long terminal repeat (LTR) being inserted into the internal repeat short (IRS) region of JM/102W. When the resulting recombinant virus was serially passed in cell culture, the initial LTR was duplicated and a second LTR spontaneously appeared in the terminal repeat short (TRS) region of the MDV genome. The virus, designated RM1, was significantly attenuated but still induced severe bursal and thymic atrophy (Isfort et al. PNAS 89:991-995). To determine whether the altered phenotype was due solely to the LTR, we cloned the LTR from the RM1 IRS region and inserted it into the IRS region of a very virulent bacterial artificial clone (BAC) of the Md5 strain of MDV, which we designated rMd5-RM1-LTR. During blind passage in duck embryo fibroblast cultures, the initial LTR in the rMd5-RM1-LTR was also duplicated, with LTRs appearing in both IRS and TRS regions of the MDV genome. The inserted LTR sequences and transcripts associated with the MDV open reading frames MDV085, MDV086, SORF2, US1, and US10 were molecularly characterized. The parental Md5 BAC contains a family of transcripts of 3, 2, and 1 kb that all terminate at the end of the US10 gene. The rMd5-RM1-LTR and RM1 viruses both express an additional 4 kb transcript that originates in the LTR and also terminates after US10. Collectively, the data suggest that our engineered rMd5-RM1-LTR virus very closely resembles the RM1 virus in its structure and transcription patterns. C1 [Kim, Taejoong; Mays, Jody; Fadly, Aly; Silva, Robert F.] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. [Kim, Taejoong] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. RP Silva, RF (reprint author), ARS, Avian Dis & Oncol Lab, USDA, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM Bob.Silva@ars.usda.gov NR 39 TC 9 Z9 12 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD JUN PY 2011 VL 42 IS 3 BP 369 EP 376 DI 10.1007/s11262-011-0585-9 PG 8 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 775VX UT WOS:000291492600009 PM 21340512 ER PT J AU Cohnstaedt, LW Beati, L Caceres, AG Ferro, C Munstermann, LE AF Cohnstaedt, Lee W. Beati, Lorenza Caceres, Abraham G. Ferro, Cristina Munstermann, Leonard E. TI Phylogenetics of the Phlebotomine Sand Fly Group Verrucarum (Diptera: Psychodidae: Lutzomyia) SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID FLIES DIPTERA; SYSTEMATIC RELATIONSHIPS; GENETIC-RELATIONSHIPS; SPECIES GROUP; DNA; SEQUENCES; COLOMBIA; VECTORS; INTROGRESSION; LONGIPALPIS AB Within the sand fly genus Lutzomyia, the Verrucarum species group contains several of the principal vectors of American cutaneous leishmaniasis and human bartonellosis in the Andean region of South America. The group encompasses 40 species for which the taxonomic status, phylogenetic relationships, and role of each species in disease transmission remain unresolved. Mitochondrial cytochrome c oxidase I (COI) phylogenetic analysis of a 667-bp fragment supported the morphological classification of the Verrucarum group into series. Genetic sequences from seven species were grouped in well-supported monophyletic lineages. Four species, however, clustered in two paraphyletic lineages that indicate conspecificity-the Lutzomyia longiflocosa-Lutzomyia sauroida pair and the Lutzomyia quasitownserzdi-Lutzomyia torvida pair. COI sequences were also evaluated as a taxonomic tool based on interspecific genetic variability within the Verrucarum group and the intraspecific variability of one of its members, Lutzomyia verrucarum, across its known distribution. C1 [Cohnstaedt, Lee W.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Beati, Lorenza] Southern Univ, Inst Arthropodol & Parasitol, Statesboro, GA USA. [Caceres, Abraham G.] Univ Nacl, Fac Med Humana, Dept Acad Microbiol Mod, Lima, Peru. [Caceres, Abraham G.] Inst Nacl Salud, Entomol Lab, Lima, Peru. Inst Nacl Salud, Entomol Lab, Bogota, Colombia. [Munstermann, Leonard E.] Yale Univ, Yale Sch Publ Hlth, New Haven, CT USA. Georgia So Univ, Inst Arthropodol & Parasitol, Statesboro, GA 30460 USA. RP Cohnstaedt, LW (reprint author), USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM Lee.Cohnstaedt@ars.usda.gov; LorenzaBeati@Georgiasouthen.edu; agc12011@gmail.com; Leonard.Munstermann@yale.edu FU National Institutes of Health [U19-A1065866, PTG-2T32-AI07404, R01-AI56254] FX We thank Lynn A. Jones for her taxonomic expertise identifying sand flies, Dr. Virginia Hodgkinson for her comments on the manuscript, and Nicolas Patricio for his help with the sand fly capture in Peru. A special thanks to the Caccone/Powell Lab at Yale University and the Laboratorio de Entomologia del Instituto Nacional de Salud for the Colombian collections (assisted by National Institutes of Health Grant R01-AI56254). A Down's Fellowship Grant to L.W.C. funded sand fly collections in Peru, and molecular work was funded by National Institutes of Health Program Grant U19-A1065866 and Training Grant PTG-2T32-AI07404. NR 32 TC 17 Z9 17 U1 0 U2 5 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA SN 0002-9637 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD JUN PY 2011 VL 84 IS 6 BP 913 EP 922 DI 10.4269/ajtmh.2011.11-0040 PG 10 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA 773TI UT WOS:000291333200014 PM 21633028 ER PT J AU Chen, SY Wang, F Beaulieu, JC Stein, RE Ge, BL AF Chen, Siyi Wang, Fei Beaulieu, John C. Stein, Rebecca E. Ge, Beilei TI Rapid Detection of Viable Salmonellae in Produce by Coupling Propidium Monoazide with Loop-Mediated Isothermal Amplification SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID REAL-TIME PCR; LISTERIA-MONOCYTOGENES CELLS; ETHIDIUM MONOAZIDE; VIBRIO-VULNIFICUS; UNITED-STATES; DEAD CELLS; CAMPYLOBACTER-JEJUNI; RISK-ASSESSMENT; RAW OYSTERS; BACTERIA AB Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by coupling a simple propidium monoazide sample treatment with loop-mediated isothermal amplification (PMA-LAMP). We first designed and optimized a LAMP assay targeting Salmonella. Second, the performance of PMA-LAMP for detecting and quantifying viable salmonellae was determined. Finally, the assay was evaluated in experimentally contaminated produce items (cantaloupe, spinach, and tomato). Under the optimized condition, PMA-LAMP consistently gave negative results for heat-killed Salmonella cells with concentrations up to 10(8) CFU/ml (or CFU/g in produce). The detection limits of PMA-LAMP were 3.4 to 34 viable Salmonella cells in pure culture and 6.1 x 10(3) to 6.1 x 10(4) CFU/g in spiked produce samples. In comparison, PMA-PCR was up to 100-fold less sensitive. The correlation between LAMP time threshold (TT) values and viable Salmonella cell numbers was high (R(2) = 0.949 to 0.993), with a quantification range (10(2) to 10(5) CFU/reaction in pure culture and 10(4) to 10(7) CFU/g in produce) comparable to that of PMA in combination with quantitative real-time PCR (PMA-qPCR). The complete PMA-LAMP assay took about 3 h to complete when testing produce samples. In conclusion, this rapid, accurate, and simple method to detect and quantify viable Salmonella cells in produce may present a useful tool for the produce industry to better control potential microbial hazards in produce. C1 [Chen, Siyi; Wang, Fei; Ge, Beilei] Louisiana State Univ, Dept Food Sci, Baton Rouge, LA 70803 USA. [Beaulieu, John C.; Stein, Rebecca E.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Ge, BL (reprint author), Louisiana State Univ, Dept Food Sci, 111 Food Sci Bldg, Baton Rouge, LA 70803 USA. EM bge@lsu.edu RI Wang, Fei/J-4353-2014; OI Beaulieu, John/0000-0001-7767-5901 FU Center for Produce Safety at the University of California, Davis [SA7498] FX This study was supported in part by funding from the Center for Produce Safety (contract SA7498) at the University of California, Davis. NR 50 TC 58 Z9 59 U1 5 U2 26 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2011 VL 77 IS 12 BP 4008 EP 4016 DI 10.1128/AEM.00354-11 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 773WI UT WOS:000291341800013 PM 21498750 ER PT J AU Zhang, SC Ling, KS AF Zhang, Shuo Cheng Ling, Kai-Shu TI Genetic diversity of sweet potato begomoviruses in the United States and identification of a natural recombinant between sweet potato leaf curl virus and sweet potato leaf curl Georgia virus SO ARCHIVES OF VIROLOGY LA English DT Article ID MAIZE-STREAK-VIRUS; ROLLING CIRCLE AMPLIFICATION; STRANDED-DNA VIRUSES; 1ST REPORT; TOP VIRUS; GEMINIVIRUS; REPLICATION; C4; POLYMERASE; SUPPRESSOR AB In the United States, two sweet potato begomoviruses, sweet potato leaf curl virus (SPLCV) and sweet potato leaf curl Georgia virus (SPLCGV), were previously identified in Louisiana. In recent years, at least seven additional sweet potato begomoviruses have been identified in other parts of the world. In an effort to determine the genetic diversity and distribution of sweet potato begomoviruses in the U.S., we focused our efforts on molecular characterization of field-collected begomovirus isolates in two states: Mississippi and South Carolina. Using rolling-circle amplification, a total of 52 clones of the full genome were obtained. Initial inspection of alignments of the end sequences in these clones revealed a strong genetic diversity. Overall, 10 genotypes could be assigned. A majority of the isolates (50/52) in eight genotypes were shown to be closely related to SPLCV. A representative clone of each genotype was fully sequenced and analyzed. Among them, four genotypes from South Carolina with 91-92% sequence identity to the type member of SPLCV were considered a new strain, whereas four other genotypes from Mississippi with > 95% sequence identity to SPLCV were considered variants. In addition, a member of a proposed new begomovirus species was identified after comparative sequence analysis of the isolate [US:SC:646B-9] from South Carolina with less than 89% sequence identity to any known begomovirus. Hence, the provisional name Sweet potato leaf curl South Carolina virus (SPLCSCV) is proposed. Moreover, a natural recombinant consisting of two distinct parental genomic sequences from SPLCV and SPLCGV was identified in the sample [US:MS:1B-3] from Mississippi. Two recombinant breakpoints were identified, one in the origin of replication and the other between C2 and C4. This knowledge about the genetic diversity of begomoviruses infecting sweet potato will likely have a major impact on PCR-based virus detection and on disease management practice through breeding for virus resistance. C1 [Ling, Kai-Shu] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Zhang, Shuo Cheng] Alcorn State Univ, Ctr Biotechnol & Genom, Alcorn State, MS 39096 USA. RP Ling, KS (reprint author), ARS, US Vegetable Lab, USDA, 2700 Savannah Highway, Charleston, SC 29414 USA. EM kai.ling@ars.usda.gov FU USDA-NIFA FX We thank Dr. D. Michael Jackson and Alvin Simmons for critical reading of the manuscript, and Andrea Gilliard and Kim Bowie for excellent technical assistance. Funding for this research was supported in part by a USDA-NIFA grant. NR 68 TC 8 Z9 12 U1 4 U2 20 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD JUN PY 2011 VL 156 IS 6 BP 955 EP 968 DI 10.1007/s00705-011-0930-2 PG 14 WC Virology SC Virology GA 769ZL UT WOS:000291056700004 PM 21302123 ER PT J AU Xie, CH Kang, J Burris, R Ferguson, ME Schauss, AG Nagarajan, S Wu, XL AF Xie, Chenghui Kang, Jie Burris, Ramona Ferguson, Matthew E. Schauss, Alexander G. Nagarajan, Shanmugam Wu, Xianli TI Acai juice attenuates atherosclerosis in ApoE deficient mice through antioxidant and anti-inflammatory activities SO ATHEROSCLEROSIS LA English DT Article DE Acai; Euterpe oleracea Mart.; Inflammation; Antioxidant enzyme; apoE-deficient mice; Atherosclerosis; Cytokine ID CORONARY-ARTERY-DISEASE; PEROXIDATION IN-VIVO; LIPID-PEROXIDATION; ENZYME EXPRESSION; OXIDATIVE STRESS; INFLAMMATION; MECHANISMS; ACID; PRODUCTS; MARKERS AB Objective: Acai fruit pulp has received much attention because of its high antioxidant capacity and potential anti-inflammatory effects. In this study, athero-protective effects of acai juice were investigated in apolipoprotein E deficient (apoE(-/-)) mice. Methods and results: ApoE(-/-) mice were fed AIN-93G diet (CD) or CD formulated to contain 5% freeze-dried acai juice powder (AJ) for 20 weeks. The mean lesion areas in the aorta for apoE(-/-) mice fed AJ were 58% less (P < 0.001) compared to that for CD fed mice. HDL-cholesterol was higher in AJ fed mice. Biomarkers of lipid peroxidation, including F(2)-isoprostanes and isomers of hydroxyoctadecadienoic acids and hydroxyeicosatetraenoic acids were significantly lower in serum and in liver of AJ fed mice. Expression of the two antioxidant enzyme genes, Gpx3 and Gsr, were significantly up-regulated in the aorta from AJ fed mice. The activity of GPX, GSR and PON1 increased in serum and/or liver of mice fed AJ. In the second experiment, ApoE(-/-) mice were fed CD or AJ for 5 weeks. Serum levels, gene expression and protein levels of the two proinflammatory cytokines TNF-alpha and IL-6 in the resident macrophages with or without LPS stimulation were lower in mice fed AJ. SEAP reporter assay determined that AJ reduced NF-kappa B activation. Conclusion: Reducing lipid peroxidation through boosting antioxidant enzymes and inhibiting pro-inflammatory cytokine production are proposed as major underlying mechanisms for the athero-protective effects of the acai juice tested in these experimental in vivo models. (C) 2011 Elsevier Ireland Ltd. All rights reserved. C1 [Xie, Chenghui; Kang, Jie; Wu, Xianli] Univ Arkansas Med Sci, Dept Physiol & Biophys, Little Rock, AR 72202 USA. [Xie, Chenghui; Kang, Jie; Burris, Ramona; Ferguson, Matthew E.; Nagarajan, Shanmugam; Wu, Xianli] Univ Arkansas Med Sci, USDA Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA. [Schauss, Alexander G.] AIBMR Life Sci Inc, Puyallup, WA 98373 USA. [Nagarajan, Shanmugam] Univ Arkansas Med Sci, Dept Immunol, Little Rock, AR 72202 USA. RP Wu, XL (reprint author), Univ Arkansas Med Sci, Dept Physiol & Biophys, 15 Childrens Way, Little Rock, AR 72202 USA. EM wuxianli@uams.edu FU MonaVie LLC (South Jordan, UT, USA) FX The authors express our appreciation for financial support by MonaVie LLC (South Jordan, UT, USA). NR 36 TC 30 Z9 30 U1 0 U2 23 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 J9 ATHEROSCLEROSIS JI Atherosclerosis PD JUN PY 2011 VL 216 IS 2 BP 327 EP 333 DI 10.1016/j.atherosclerosis.2011.02.035 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 773XA UT WOS:000291343600014 PM 21411096 ER PT J AU Dien, BS Miller, DJ Hector, RE Dixon, RA Chen, F McCaslin, M Reisen, P Sarath, G Cotta, MA AF Dien, Bruce S. Miller, David J. Hector, Ronald E. Dixon, Richard A. Chen, Fang McCaslin, Mark Reisen, Peter Sarath, Gautam Cotta, Michael A. TI Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition SO BIORESOURCE TECHNOLOGY LA English DT Article DE Biomass; Alfalfa; Bioethanol; Pretreatment; Lignin-modified transgenics ID CAFFEIC ACID 3-O-METHYLTRANSFERASE; BIOFUEL PRODUCTION; TRANSGENIC ALFALFA; FUEL ETHANOL; BIOMASS; SACCHARIFICATION; BIOSYNTHESIS; IMPACTS; FERMENTATION; PRETREATMENT AB Alfalfa (Medicago sativa L) biomass was evaluated for biochemical conversion into ethanol using dilute-acid and ammonia pretreatments. The two alfalfa lines compared were a reduced S-lignin transgenic cultivar generated through down regulation of the caffeic acid O-methyltransferase gene and a wild-type control. Both were harvested at two maturities. All the samples had similar carbohydrate contents including a mean composition of 316 g glucan and 497 g total neutral carbohydrates per kg dry biomass, which corresponds to a theoretic ethanol yield of 3821/ton. Ethanol yields for alfalfa stems pretreated with dilute-acid were significantly impacted by harvest maturity and lignin composition, whereas when pretreated with dilute-ammonia, yield was solely affected by lignin composition. Use of a recombinant xylose-fermenting Saccharomyces strain, for converting the ammonia pretreated alfalfa samples, further increased ethanol yields. Ethanol yields for the xylose-fermenting yeast were 232-278 l/ton and were significantly enhanced for the reduced S lignin cultivars. Published by Elsevier Ltd. C1 [Dien, Bruce S.; Hector, Ronald E.; Cotta, Michael A.] ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Miller, David J.] Pioneer HiBred Int Inc, Arlington, WI 53911 USA. [Dixon, Richard A.; Chen, Fang] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA. [McCaslin, Mark; Reisen, Peter] Forage Genet, Nampa, ID 83651 USA. [Sarath, Gautam] Univ Nebraska, Forage & Bioenergy Res Unit, USDA, ARS, Lincoln, NE 68583 USA. RP Dien, BS (reprint author), ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM Bruce.Dien@ars.usda.gov OI Cotta, Michael/0000-0003-4565-7754; Dien, Bruce/0000-0003-3863-6664 FU Forage Genetics International FX The authors would like to thank Ms. Patricia J. O'Bryan, Mr. Loren Iten, and Nathan A. Palmer for their fine technical assistance. The authors also thank Ms. Debra Plamquist for statistical help. The work was supported by grants from Forage Genetics International (to Richard A. Dixon). NR 35 TC 40 Z9 44 U1 0 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUN PY 2011 VL 102 IS 11 BP 6479 EP 6486 DI 10.1016/j.biortech.2011.03.022 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 771XU UT WOS:000291194800016 PM 21474304 ER PT J AU Adey, WH Kangas, PC Mulbry, W AF Adey, Walter H. Kangas, Patrick C. Mulbry, Walter TI Algal Turf Scrubbing: Cleaning Surface Waters with Solar Energy while Producing a Biofuel SO BIOSCIENCE LA English DT Article DE algae; biofuel; ecological engineering; nitrogen; phosphorus ID DAIRY MANURE EFFLUENT; LOADING RATES; NUTRIENT REMOVAL; PRODUCTIVITY; BIOMASS; MICROALGAE; RECOVERY; STREAMS; SYSTEM AB As human populations have expanded, Earth's atmosphere and natural waters have become dumps for agricultural and industrial wastes. Remediation methods of the last half century have been largely unsuccessful. In many US watersheds, surface waters are eutrophic, and coastal water bodies, such as the Chesapeake Bay and the Gulf of Mexico, have become increasingly hypoxic. The algal turf scrubber (ATS) is an engineered system for flowing pulsed wastewaters over sloping surfaces with attached, naturally seeded filamentous algae. This treatment has been demonstrated for tertiary sewage, farm wastes, streams, and large aquaculture systems; rates as large as 40 million to 80 million liters per day (lpd) are routine. Whole-river-cleaning systems of 12 billion lpd are in development. The algal biomass, produced at rates 5 to 10 times those of other types of land-based agriculture, can be fermented, and significant research and development efforts to produce ethanol, butanol, and methane are under way. Unlike with algal photobioreactor systems, the cost of producing biofuels from the cleaning of wastewaters by ATS can be quite low. C1 [Adey, Walter H.] Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, Washington, DC 20560 USA. [Kangas, Patrick C.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Mulbry, Walter] ARS, Environm Management & Byprod Utilizat Lab, USDA, Beltsville, MD USA. RP Adey, WH (reprint author), Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, Washington, DC 20560 USA. EM adeyw@si.edu FU Lewis Foundation FX We gratefully acknowledge HydroMentia, Inc., for providing figures 4 and 5 and John Miller (Western Michigan University) for providing information for table 1. Funding was provided by a gift from the Lewis Foundation to the Smithsonian Institution. J. Emmett Duffy and Raleigh Hood provided many constructive comments on the manuscript and submitted supporting letters in an early stage of manuscript development. Their insightful comments were crucial to the development of a cogent manuscript. NR 51 TC 34 Z9 35 U1 7 U2 76 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD JUN PY 2011 VL 61 IS 6 BP 434 EP 441 DI 10.1525/bio.2011.61.6.5 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 773TM UT WOS:000291333700005 ER PT J AU Li, GY Huang, LH Hse, CY Qin, TF AF Li, Gai-Yun Huang, Luo-Hua Hse, Chung-Yun Qin, Te-Fu TI Chemical compositions, infrared spectroscopy, and X-ray diffractometry study on brown-rotted woods SO CARBOHYDRATE POLYMERS LA English DT Article DE Brown-rot decay; Chemical component; Alkali solubility; Crystallinity; FTIR ID WHITE-ROT; FUNGI; CELLULOSE; DECAY; CRYSTALLINITY; LIGNIN; FIBERS; FTIR AB The effect of brown-rot decay on the chemical composition and crystallinity of Masson pine was studied by exposing it to Wolfiporia cocos (Schwein.) Ryvarden and Gilbn. for durations of up to 15 weeks in the field. The holocellulose content, alpha-cellulose content, and wood crystallinity decreased slowly in the initial stage, followed by a significant reduction during the late stage. Pentosans did not have a significant reduction until 15-week decay, indicating that pentosans were degraded much more slowly than the cellulose. Strong correlations between the holocellulose and lignin content and alkali solubility, wood crystallinity, and IR band height ratio indicated that different wood properties affected each other during the degradation process. The results indicated that the slight degradation of amorphous cellulose and hemicellulose formed the entrance points for W. cocos to attack crystalline cellulose, and led to the serious degradation of the cell wall. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Li, Gai-Yun; Huang, Luo-Hua; Qin, Te-Fu] Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. [Hse, Chung-Yun] USDA Forest Serv, So Res Stn, Pineville, LA 71360 USA. RP Qin, TF (reprint author), Chinese Acad Forestry, Res Inst Wood Ind, Xiangshan Rd, Beijing 100091, Peoples R China. EM tefuqin@yahoo.cn FU National Key Technologies R&D Program for the 11th Five-Year Plan [2006BAD18B1002, 2006BAD03A16] FX The financial support of National Key Technologies R&D Program for the 11th Five-Year Plan (project number: 2006BAD18B1002 and 2006BAD03A16) are gratefully acknowledged. NR 23 TC 16 Z9 16 U1 2 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JUN 1 PY 2011 VL 85 IS 3 BP 560 EP 564 DI 10.1016/j.carbpol.2011.03.014 PG 5 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 770WT UT WOS:000291119900011 ER PT J AU Anderson, DP Sturtevant, BR AF Anderson, Dean P. Sturtevant, Brian R. TI Pattern analysis of eastern spruce budworm Choristoneura fumiferana dispersal SO ECOGRAPHY LA English DT Article ID POPULATION GENETIC-STRUCTURE; TENT CATERPILLAR OUTBREAKS; ANEMOCHOROUS FOREST TREES; SPATIAL SYNCHRONY; BALSAM FIR; MULTILOCUS GENOTYPES; INSECT OUTBREAKS; MOTH LEPIDOPTERA; TRAVELING-WAVES; SEED DISPERSAL AB Dispersal has been proposed as an important mechanism in the broad-scale synchronisation of insect outbreaks by linking spatially disjunct populations. Evidence suggests that dispersal is influenced by landscape structure, phenology, temperature, and air currents; however, the details remain unclear due to the difficulty of quantifying dispersal. In this study, we used data on the abundance and distribution of spruce budworm Choristoneura fumiferana larvae (potential dispersers) and adult male moths (dispersers) to make inference on the effects of air currents and host-species abundance on dispersal. Hierarchical-Bayesian and inverse modeling was used to explore 4 dispersal models: 1) isotropic dispersal; 2) directional-dispersal; 3) directional-and-host-species dispersal; and 4) host-species dispersal. Despite their strong dependence on balsam fir Abies balsamea and spruce species Picea spp., the mapped basal area of these host species did not influence the pattern of dispersed moths. The model that best fit the data was the directional-dispersal model, which showed that the prevailing dispersal direction was from the northwest (328 degrees). We infer that the strong pattern of directional dispersal was due to a prevailing wind from the same direction. Our interpretation was corroborated by independent wind data during the period of active adult male budworm flight, particularly in the region with high larval abundance. Our results indicate that there was a relatively high probability of individuals flying at least 48 km with the wind where larvae abundance at source locations was also high. Such findings emphasize the importance of long-distance dispersal on spatial distribution of adult male spruce budworms. Insight into the population-level consequences of such dispersal patterns requires additional research. C1 [Anderson, Dean P.] Landcare Res, Lincoln, New Zealand. [Sturtevant, Brian R.] US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. RP Anderson, DP (reprint author), Landcare Res, POB 40, Lincoln, New Zealand. FU United States Dept of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative [WISR-2005-02815]; National Fire Plan FX This work was supported by the United States Dept of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative Grant no. WISR-2005-02815, and the National Fire Plan. We would to thank Barry Cooke for his guidance in field methods and SBW identification, and also the following people for assisting with the field data collection: Nate Aspelin, Louis-Etienne Robert, Brian Miranda, Robert Costanza, Barry Cooke, Stephane Bourassa, and Patrick James. The Superior National Forest provided housing during the field season and assisted with sampling logistics. - Remi St-Amant assemble the necessary weather input data required to estimate the budworm adult flight window using BioSIM. Anouk Wanrooy assisted with the figures. We also thank Marie-Josee Fortin, Mandy Barron and 3 anonymous reviewers for helpful comments on the manuscript. NR 59 TC 13 Z9 13 U1 2 U2 36 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0906-7590 J9 ECOGRAPHY JI Ecography PD JUN PY 2011 VL 34 IS 3 BP 488 EP 497 DI 10.1111/j.1600-0587.2010.06326.x PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 772IT UT WOS:000291228300014 ER PT J AU Moser, BR AF Moser, Bryan R. TI Complementary blending of meadowfoam seed oil methyl esters with biodiesel prepared from soybean and waste cooking oils to enhance fuel properties SO ENERGY & ENVIRONMENTAL SCIENCE LA English DT Article ID VEGETABLE-OILS; FATTY-ACID; OXIDATIVE STABILITY; FLOW PROPERTIES; ANTIOXIDANT; TOCOPHEROLS; MIXTURES; STORAGE; CANOLA; LIPIDS AB The complementary blending of cold-pressed meadowfoam seed oil methyl esters (MFME) with soybean and waste cooking oil methyl esters (SME and WCME) was investigated. MFME prepared from cold-pressed meadowfoam oil exhibited an exceptionally high induction period (IP) of 66.2 h whereas SME and WCME yielded considerably lower values of 5.9 and 4.5 h, respectively. The kinematic viscosities (KVs) of SME and WCME were within the prescribed ranges in the biodiesel standards (ASTM D6751 and EN 14214) but MFME was not due to its content of unique longer-chain fatty acids. Furthermore, the iodine value (IV) of SME was in excess of the maximum limit specified in EN 14214 whereas MFME and WCME were not. Lastly, the cold flow properties of MFME were superior to those of SME and WCME. Complementary blends of MFME-SME and MFME-WCME ameliorated deficiencies of the individual fuels. Blends containing 20-30 vol % MFME in WCME provided IPs, KVs and IVs within the limits prescribed in both ASTM D6751 and EN 14214. With respect to MFME-SME blends, those containing 30-50% MFME complied with the specifications of both biodiesel standards. In addition, highly linear correlations between percentage of MFME and fuel properties were established. Furthermore, the influence of storage for 90 days on fuel properties of the blends was determined. C1 ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Moser, BR (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov NR 33 TC 7 Z9 7 U1 1 U2 8 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 1754-5692 J9 ENERG ENVIRON SCI JI Energy Environ. Sci. PD JUN PY 2011 VL 4 IS 6 BP 2160 EP 2167 DI 10.1039/c1ee01047f PG 8 WC Chemistry, Multidisciplinary; Energy & Fuels; Engineering, Chemical; Environmental Sciences SC Chemistry; Energy & Fuels; Engineering; Environmental Sciences & Ecology GA 772FG UT WOS:000291219100028 ER PT J AU Kopittke, PM Kinraide, TB Wang, P Blarney, FPC Reichman, SM Menzies, NW AF Kopittke, Peter M. Kinraide, Thomas B. Wang, Peng Blarney, F. Pax C. Reichman, Suzie M. Menzies, Neal W. TI Alleviation of Cu and Pb Rhizotoxicities in Cowpea (Vigna unguiculata) as Related to Ion Activities at Root-Cell Plasma Membrane Surface SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID BIOTIC LIGAND MODEL; BARLEY HORDEUM-VULGARE; PREDICTING NICKEL TOXICITY; LOW PH; COPPER; ALUMINUM; BIOAVAILABILITY; MAGNESIUM; CALCIUM; METALS AB Cations, such as Ca and Mg, are generally thought to alleviate toxicities of trace metals through sites-pecific competition (as incorporated in the biotic ligand model, BLM). Short-term experiments were conducted with cowpea (Vigna unguiculata L. Walp.) seedlings in simple nutrient solutions to examine the alleviation of Cu and Pb toxicities by Al, Ca, H, Mg, and Na. For Cu, the cations depolarized the plasma membrane (PM) and reduced the negativity of psi(o)(0) (electrical potential at the outer surface of the PM) and thereby decreased {Cu(2+)}(0)(o) (activity of Cu(2+) at the outer surface of the PM). For Pb, root elongation was generally better correlated to the activity of Pb(2+) in the bulk solution than to {Pb2+}(0)(o). However, we propose that the addition of cations resulted in a decrease in {Pb(2+)}(0)(o) but a simultaneous increase in the rate of Pb uptake (due to an increase in the negativity of E(m,surb) the difference in potential between the inner and outer surfaces of the PM) thus offsetting the decrease in {Pb(2+)}(0)(o). In addition, Ca was found to alleviate Pb toxicity through a specific effect. Although our data do not preclude site-specific competition (as incorporated in the BLM), we suggest that electrostatic effects have an important role. C1 [Kopittke, Peter M.; Blarney, F. Pax C.; Menzies, Neal W.] Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld 4072, Australia. [Kopittke, Peter M.; Blarney, F. Pax C.; Menzies, Neal W.] Univ Queensland, CRC CARE, St Lucia, Qld 4072, Australia. [Kinraide, Thomas B.] ARS, USDA, Beaver, WV 25813 USA. [Wang, Peng] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China. [Reichman, Suzie M.] RMIT Univ, Sch Civil Environm & Chem Engn, Melbourne, Vic 3001, Australia. RP Kopittke, PM (reprint author), Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld 4072, Australia. EM p.kopittke@uq.edu.au RI Wang, Peng/E-2008-2012; Kopittke, Peter/A-6026-2011; Menzies, Neal/L-1338-2013; OI Wang, Peng/0000-0001-8622-8767; Kopittke, Peter/0000-0003-4948-1880; Menzies, Neal/0000-0003-0207-070X; Reichman, Suzie/0000-0002-5110-5166 FU Environmental Protection Authority (EPA), Victoria, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE) [3-03-05-09/10] FX The authors acknowledge the support of the Environmental Protection Authority (EPA), Victoria, Australia. This research was funded through the Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE) Project 3-03-05-09/10. Statement of Equal Opportunity and Product Disclaimer: The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products does no: imply recommendation or endorsement by the USDA. NR 22 TC 28 Z9 30 U1 1 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 1 PY 2011 VL 45 IS 11 BP 4966 EP 4973 DI 10.1021/es1041404 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 771AD UT WOS:000291128700044 PM 21563792 ER PT J AU Marian, C Tao, M Mason, JB Goerlitz, DS Nie, J Chanson, A Freudenheim, JL Shields, PG AF Marian, C. Tao, M. Mason, J. B. Goerlitz, D. S. Nie, J. Chanson, A. Freudenheim, J. L. Shields, P. G. TI Single nucleotide polymorphisms in uracil-processing genes, intake of one-carbon nutrients and breast cancer risk SO EUROPEAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE uracil-processing genes; single nucleotide polymorphisms; breast cancer ID EXCISION-REPAIR PATHWAY; LUNG-CANCER; DNA-URACIL; POPULATION; FOLATE; DAMAGE; MISINCORPORATION; CARCINOGENESIS; CONSEQUENCES; DEFICIENCIES AB Background/Objectives: The misincorporation of uracil into DNA leads to genomic instability. In a previous study, some of us identified four common single nucleotide polymorphisms (SNPs) in uracil-processing genes (rs2029166 and rs7296239 in SMUG1, rs34259 in UNG and rs4775748 in DUT) that were associated with significantly altered levels of uracil in human DNA. We investigated whether any of these SNPs are associated with an altered risk of developing breast cancer and if one-carbon nutrients intake can modify their effects. Subjects/Methods: We genotyped the four SNPs in 1077 cases of incident breast cancer and 1910 age and race-matched controls in the Western New York Exposures and Breast Cancer (WEB) Study and examined associations with breast cancer risk and interactions with intake of folate, vitamins B6 and B12. Results: After adjustment for known risk factors for breast cancer, there was increased risk of breast cancer among postmenopausal women who were heterozygous for either of the two SMUG1 SNPs (odds ratio (OR) 1.29, 95% confidence interval (CI) 1.07-1.56) and OR 1.29, 95% CI 1.07-1.55, respectively). Among premenopausal women, increased risk associated with the SMUG1 rs2029166 genotype was limited to those with low folate intake. There were no other interactions with vitamins B(6) or B(12) intake. Conclusions: Our study suggests that the four selected SNPs are not robust determinants of breast cancer risk, but that the two SNPs in SMUG1 might modestly alter the risk of breast cancer. However, the increase in risk among heterozygotes in the two SNPs in SMUG1, which is thought to be the most active glycosylase in vivo, raises the possibility that subtle 'heterosis' effects on cancer risk might be produced by these SNPs. European Journal of Clinical Nutrition (2011) 65, 683-689; doi:10.1038/ejcn.2011.29; published online 23 March 2011 C1 [Marian, C.] Georgetown Univ, Carcinogenesis Biomarkers & Epidemiol Program, Lombardi Comprehens Canc Ctr, Med Ctr, Washington, DC 20057 USA. [Tao, M.; Nie, J.; Freudenheim, J. L.] SUNY Buffalo, Sch Publ Hlth & Hlth Profess, Dept Social & Prevent Med, Buffalo, NY 14260 USA. [Mason, J. B.; Chanson, A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Vitamins & Carcinogenesis Lab, Boston, MA 02111 USA. RP Marian, C (reprint author), Georgetown Univ, Carcinogenesis Biomarkers & Epidemiol Program, Lombardi Comprehens Canc Ctr, Med Ctr, 3800 Reservoir Rd,NW LLs Level,Room 116A, Washington, DC 20057 USA. EM cm444@georgetown.edu RI Shields, Peter/I-1644-2012; Marian, Catalin/B-1750-2012; OI Marian, Catalin/0000-0002-7749-1384 FU NCRR NIH HHS [M01 RR020359, P41 RR013461] NR 31 TC 9 Z9 9 U1 1 U2 2 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0954-3007 J9 EUR J CLIN NUTR JI Eur. J. Clin. Nutr. PD JUN PY 2011 VL 65 IS 6 BP 683 EP 689 DI 10.1038/ejcn.2011.29 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 772FD UT WOS:000291218800004 PM 21427733 ER PT J AU DeSantis, RD Hallgren, SW Stahle, DW AF DeSantis, Ryan D. Hallgren, Stephen W. Stahle, David W. TI Drought and fire suppression lead to rapid forest composition change in a forest-prairie ecotone SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Drought; Fire exclusion; Juniperus virginiana; Quercus marilandica; Quercus stellata; Recruitment ID RED CEDAR JUNIPERUS; EASTERN REDCEDAR; TALLGRASS PRAIRIE; GRADIENT ANALYSIS; CENTRAL MISSOURI; PLANT COMMUNITY; UPLAND FORESTS; CROSS TIMBERS; NORTH-AMERICA; OAK QUERCUS AB Altered fire regimes and increased drought can lead to major vegetation changes, especially in ecotones. A decrease in fire can lead to woody species encroachment in prairies and increasing forest stand density. The threat of global climate change raises questions about potential increases in the length, severity, and incidence of droughts substantially altering species composition. Re-measured upland forests in south-central North America's midcontinent forest-prairie ecotone exhibited major changes in woody species composition and structure over fifty years and successional trajectories appeared to favor invasive Juniperus virginiana L over the previous dominant Quercus species. The objective of this study was to determine whether climate and fire exclusion affected the recruitment history of dominant woody species in these upland forests located near the xeric western edge of the eastern deciduous forest biome of North America. We removed cores and cross-sections from 992 J. virginiana, Quercus marilandica Munchh. and Q. stellata Wangenh. trees from eleven forest stands located across central and northwest Oklahoma, and determined their ages using standard dendrochronological methods. Recruitment of all species increased following a severe mid-20th century drought, but a rapid increase in J. virginiana recruitment and decrease in Quercus recruitment appeared to be linked to a decrease in fire. Future fire regime changes and increased drought due to global climate change could lead to widespread shifts from Quercus-to Juniperus-dominated forests and cause substantial changes to ecosystem services. (C) 2011 Elsevier B.V. All rights reserved. C1 [DeSantis, Ryan D.; Hallgren, Stephen W.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. [Stahle, David W.] Univ Arkansas, Dept Geosci, Fayetteville, AR 72701 USA. RP DeSantis, RD (reprint author), US Forest Serv, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM desantisr@missouri.edu; steve.hallgren@okstate.edu; dstahle@uark.edu RI Hallgren, Stephen/A-2131-2013 FU Oklahoma State University Department of Natural Resource Ecology Management; Oklahoma State University Cooperative Extension Service and Agricultural Experiment Station through a Division of Agricultural Sciences and Natural Resources FX We thank the landowners for access to the study sites. Dr. Tom Lynch and Dr. Mike Palmer, Oklahoma State University, provided valuable advice. Thanks to Jake Beale, Kyle Hesse, and Jesse Martin for field and laboratory assistance. Financial support was provided by the Oklahoma State University Department of Natural Resource Ecology & Management and the Oklahoma State University Cooperative Extension Service and Agricultural Experiment Station through a Division of Agricultural Sciences and Natural Resources Team Initiative Project grant. NR 84 TC 21 Z9 22 U1 13 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2011 VL 261 IS 11 BP 1833 EP 1840 DI 10.1016/j.foreco.2011.02.006 PG 8 WC Forestry SC Forestry GA 770YZ UT WOS:000291125700013 ER PT J AU Coleman, TW Grulke, NE Daly, M Godinez, C Schilling, SL Riggan, PJ Seybold, S AF Coleman, Tom W. Grulke, Nancy E. Daly, Miles Godinez, Cesar Schilling, Susan L. Riggan, Philip J. Seybold, Steven. TI Coast live oak, Quercus agrifolia, susceptibility and response to goldspotted oak borer, Agrilus auroguttatus, injury in southern California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Invasive species; Buprestidae; Phloem/wood borer; Tree health rating; Tree drought stress; Tree physiology; Water use efficiency; Xylem water potential ID CHESTNUT BORER; PONDEROSA PINE; DEFOLIATED OAK; NORTH-AMERICA; TREE-AGE; BUPRESTIDAE; MORTALITY; DROUGHT; DECLINE; SOIL AB Oak mortality is often associated with a complex of decline factors. We describe the morphological and physiological responses of coast live oak, Quercus agrifolia Nee, in California to an invasive insect, the goldspotted oak borer (GSOB), Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), and evaluate drought as a potential inciting factor. Morphological traits of 356 trees were assessed and physiological traits of 70 of these were monitored intensively over one growing season. Morphological characteristics of tree health included crown thinning and dieback; bole staining resulting from larval feeding; density of GSOB adult exit holes; and holes caused by woodpecker feeding. These characteristics were used to rank GSOB infestation/injury into four classes, and taken together, they explained 87% of the variation in a principal component analysis. Drought stress on various size/age and infestation classes of Q. agrifolia was measured by assessing branchlet pre-dawn and solar noon xylem water potential, leaf cell turgor potential, and water use efficiency over one growing season. Both morphological and physiological traits were highly variable in mature and old growth trees. Early summer plant water status (branchlet xylem water potential and water use efficiency) was similar between uninfested and newly colonized trees, suggesting that GSOB are not pre-selecting drought-stressed Q agrifolia for oviposition. By late summer, leaf water and cell turgor potentials were lower in infested than in uninfested mature trees, suggesting that GSOB infestation causes drought stress in these trees. Among the tree size/age classes, infested old growth trees exhibited the greatest change in water use efficiency over the growing season, and showed greater morphological injury symptoms of decline than infested mature trees. Morphological attributes of decline in Q. agrifolia associated with GSOB were correlated weakly with increasing physiological drought stress among infestation classes of trees. We propose that the collection of morphological responses of Q. agrifolia to GSOB described here can be used to monitor the future expansion of the GSOB distribution as well as the GSOB-induced decline of Q. agrifolia in California. Published by Elsevier B.V. C1 [Coleman, Tom W.] US Forest Serv, USDA, San Bernardino, CA 92408 USA. [Grulke, Nancy E.; Godinez, Cesar; Schilling, Susan L.; Riggan, Philip J.] US Forest Serv, USDA, Pacific SW Res Stn, Riverside, CA 92507 USA. [Seybold, Steven.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95616 USA. RP Coleman, TW (reprint author), US Forest Serv, USDA, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. EM twcoleman@fs.fed.us FU USDA Forest Service Pacific Southwest Research Station and Region 5, Forest Health Protection FX This research was supported by the USDA Forest Service Pacific Southwest Research Station and Region 5, Forest Health Protection. We thank the late A. Koonce for raising awareness of oak mortality in San Diego County and for her support of this project. We also thank Stacy Hishinuma (UC-Davis Department of Entomology), Andreana Cipollone (USDA Forest Service), and Karl and David Marrett for field assistance. This work would not have been feasible without the support of the Cleveland National Forest and Viejas and La Posta Tribes. Andrew D. Graves (USDA Forest Service Forest Health Protection) and two anonymous reviewers provided valuable comments to this manuscript. NR 67 TC 24 Z9 24 U1 2 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2011 VL 261 IS 11 BP 1852 EP 1865 DI 10.1016/j.foreco.2011.02.008 PG 14 WC Forestry SC Forestry GA 770YZ UT WOS:000291125700015 ER PT J AU Pinto, JR Marshall, JD Dumroese, RK Davis, AS Cobos, DR AF Pinto, Jeremiah R. Marshall, John D. Dumroese, R. Kasten Davis, Anthony S. Cobos, Douglas R. TI Establishment and growth of container seedlings for reforestation: A function of stocktype and edaphic conditions SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Ponderosa pine; Volumetric soil moisture content; Morphology; Container seedlings; Seedling establishment and growth ID PONDEROSA PINE-SEEDLINGS; PLANTATION ESTABLISHMENT; FIELD PERFORMANCE; PLANTING STOCK; ROOT-GROWTH; WATER; STRESS; SPRUCE; COMPETITION; MORPHOLOGY AB A properly selected stocktype can greatly enhance reforestation success through increased survival and growth following outplanting. Implementing a robust stocktype trial using stocktypes of equal quality can ensure results lead to the best choice. Six container types, differing primarily in depth and volume, were used to evaluate the performance of ponderosa pine (Pinus ponderosa Laws. var. ponderosa) seedlings out-planted on two sites that varied in volumetric soil moisture content (theta), average temperature, and total precipitation (mesic and xeric). Seedlings in each container type were cultured specifically to achieve uniform seedling quality. After two growing seasons, seedlings planted at the mesic site showed high survival (>99%) and incremental growth gains of 147, 100, and 794% for height, root-collar diameter (RCD), and stem volume, respectively; container types exhibited differences in total height, RCD, and stem volume with larger containers generally yielding the largest seedlings. Seedlings planted at the xeric site experienced 83% survival, smaller growth gains (25, 46, and 220% for height, RCD, and stem volume, respectively), and also exhibited differences in height, RCD, and stem volume. Regression analysis revealed that for each site, initial seedling morphological characteristics were better at predicting absolute height, RCD and stem volume after the first year than after the second year, with initial seedling height offering the best predictive power (R-2 = 0.66, mesic site; and R-2 = 0.70, xeric site). Second-year absolute growth prediction was poorest on the mesic site (R-2 <0.21). Regression analysis indicates that initial seedling characteristics lost predictive value with time, especially on the mesic site, as seedlings grew out of their initial, container-induced characteristics and become more limited by current environmental and genetic factors. Conversely, on a xeric site, where absolute growth was reduced, traits determined by the container type persisted longer. Selecting stocktypes for mesic site conditions may only be limited by the minimum growth gains desired. Conversely, xeric sites may benefit from deep-planted quality seedlings or carefully planted long-rooted, large container seedlings. Published by Elsevier B.V. C1 [Pinto, Jeremiah R.; Dumroese, R. Kasten] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Marshall, John D.; Davis, Anthony S.] Univ Idaho, Dept Forest Ecol & Biogeosci, Moscow, ID 83843 USA. [Cobos, Douglas R.] Decagon Devices Inc, Pullman, WA 99163 USA. RP Pinto, JR (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 1221 S Main St, Moscow, ID 83843 USA. EM jpinto@fs.fed.us FU USDA Forest Service FX Potlatch Corporation and the Confederated Tribes of the Colville Indian Reservation donated seed, planting sites, and planting help for the project. Ann Abbott and Scott Baggett provided statistical guidance. Funding support was provided by the USDA Forest Service. We are thankful to the editor and the anonymous reviewers for their constructive comments on a previous version of this manuscript. NR 61 TC 23 Z9 23 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2011 VL 261 IS 11 BP 1876 EP 1884 DI 10.1016/j.foreco.2011.02.010 PG 9 WC Forestry SC Forestry GA 770YZ UT WOS:000291125700017 ER PT J AU Collins, BJ Rhoades, CC Hubbard, RM Battaglia, MA AF Collins, Byron J. Rhoades, Charles C. Hubbard, Robert M. Battaglia, Michael A. TI Tree regeneration and future stand development after bark beetle infestation and harvesting in Colorado lodgepole pine stands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Rocky Mountain forests; Forest management; Disturbance; Mountain pine beetle; Silviculture ID YELLOWSTONE-NATIONAL-PARK; WESTERN UNITED-STATES; BRITISH-COLUMBIA; DENDROCTONUS-PONDEROSAE; SHADE TOLERANCE; FORESTS; MANAGEMENT; MORTALITY; DYNAMICS; DENSITY AB In the southern Rocky Mountains, current mountain pine beetle (Dendroctonus ponderosae Hopkins) outbreaks and associated harvesting have set millions of hectares of lodgepole pine (Pinus contorta var. latifolia Engelm. ex Wats.) forest onto new stand development trajectories. Information about immediate, post-disturbance tree regeneration will provide insight on dynamics of future stand composition and structure. We compared tree regeneration in eight paired harvested and untreated lodgepole pine stands in the Fraser Experimental Forest that experienced more than 70% overstory mortality due to beetles. New seedlings colonized both harvested and untreated stands in the first years after the beetle outbreak. In harvested areas the density of new seedlings, predominantly lodgepole pine and aspen, was four times higher than in untreated stands. Annual height growth of pine and fir advance regeneration (e.g., trees established prior to the onset of the outbreak) has doubled following overstory mortality in untreated stands. Growth simulations based on our regeneration data suggest that stand basal area and stem density will return to pre-beetle levels in untreated and harvested stands within 80-105 years. Furthermore, lodgepole pine will remain the dominant species in harvested stands over the next century, but subalpine fir will become the most abundant species in untreated areas. Owing to terrain, economic and administrative limitations, active management will treat a small fraction (<15%) of the forests killed by pine beetle. Our findings suggest that the long-term consequences of the outbreak will be most dramatic in untreated forests where the shift in tree species composition will influence timber and water production, wildfire behavior, wildlife habitat and other forest attributes. Published by Elsevier B.V. C1 [Collins, Byron J.; Rhoades, Charles C.; Hubbard, Robert M.; Battaglia, Michael A.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Rhoades, CC (reprint author), US Forest Serv, Rocky Mt Res Stn, 240 W Prospect, Ft Collins, CO 80526 USA. EM crhoades@fs.fed.us RI Collins, Byron/G-2849-2011; Hubbard, Robert/J-6059-2012 FU US Forest Service, R2 Bark Beetle Incident Team; US Forest Service; Rocky Mountain Research Station; Colorado Water Conservation Board FX Without the flexibility and assistance of Craig Magwire, Rick Cassie, Chris Oliver, Jeff Underhill and field crews of the Arapaho-Roosevelt NF, Sulphur Ranger District this research would not have been possible. Thanks to Brandt Logging for their willingness to facilitate our research. Many thanks to Clint Kyhl and Cal Wettstein of the US Forest Service, R2 Bark Beetle Incident Team, the US Forest Service, Chiefs Emergency Fund program, the Rocky Mountain Research Station and the Colorado Water Conservation Board for financial support. Thanks to Kelly Elder, Mark Dixon and Banning Starr of the Fraser Experimental Forest for the use of FEF resources and logistical support. The authors also thank Scott Baggett, Laurie Forth and David Turner for their statistical expertise, Lance Asherin for assistance with growth projections, Paula Fornwalt and two anonymous reviewers for comments on the manuscript and numerous individuals for assistance with field measurements. NR 42 TC 50 Z9 51 U1 6 U2 71 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2011 VL 261 IS 11 BP 2168 EP 2175 DI 10.1016/j.foreco.2011.03.016 PG 8 WC Forestry SC Forestry GA 770YZ UT WOS:000291125700049 ER PT J AU Lin, CL Li, HF Zhang, GZ Wei, W Zhu, XQ Li, ZP Wang, H Xu, QC Zhou, T Tian, GZ AF Lin, C. L. Li, H. F. Zhang, G. Z. Wei, W. Zhu, X. Q. Li, Z. P. Wang, H. Xu, Q. C. Zhou, T. Tian, G. Z. TI Molecular identification and characterization of a new phytoplasma strain associated with Chinese chestnut yellow crinkle disease in China SO FOREST PATHOLOGY LA English DT Article AB P>Chinese chestnut trees (Castanea mollissima BL.) planted in a suburb of Beijing, China developed symptoms including yellowing, leaf crinkling, little leaf, shortened internodes, and empty burrs. Transmission electron microscopy revealed presence of phytoplasma cells in phloem sieve elements of the symptomatic chestnut trees. Molecular cloning and sequence analysis of PCR-amplified near-full length 16S rRNA gene indicated that the phytoplasma associated with the Chinese chestnut yellow crinkle disease is closely related to Japanese chestnut witches'-broom phytoplasma. This is the first report of a phytoplasmal disease in Chinese chestnut trees. C1 [Lin, C. L.; Xu, Q. C.; Tian, G. Z.] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Lab Forest Protect State Forestry Adm, Beijing 100091, Peoples R China. [Lin, C. L.; Li, H. F.; Zhang, G. Z.; Zhou, T.] China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China. [Wei, W.] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. [Zhu, X. Q.; Li, Z. P.] Beijing Forestry Bur, Forest Pest & Dis Control & Quarantine Stn Huairo, Beijing 101400, Peoples R China. [Wang, H.] Beijing Forestry Bur, Forest Protect Stn, Beijing 100029, Peoples R China. RP Tian, GZ (reprint author), Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Key Lab Forest Protect State Forestry Adm, Beijing 100091, Peoples R China. EM tiangz@caf.ac.cn FU National Natural Science Foundation of China [30471393]; Ministry of Science and Technology [2005DKA21207] FX This work was supported by National Natural Science Foundation of China (No. 30471393) and Natural Resource Platform Project from Ministry of Science and Technology (2005DKA21207). We thank Professors R. X. Shen, G. M. Huan and W. Yang, Beijing Forestry University, Director W. Q. Tao, Beijing Forestry Bureau, and Mr X.J. Wang, Huairou Bohai Station of Forest Protection for contribution to field investigation, and the electron microscope laboratory of China Agricultural University for preparation of ultra-thin section. NR 15 TC 0 Z9 0 U1 1 U2 5 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1437-4781 J9 FOREST PATHOL JI Forest Pathol. PD JUN PY 2011 VL 41 IS 3 BP 233 EP 236 DI 10.1111/j.1439-0329.2010.00679.x PG 4 WC Forestry SC Forestry GA 772QV UT WOS:000291250600010 ER PT J AU Chaston, JM Dillman, AR Shapiro-Ilan, DI Bilgrami, AL Gaugler, R Hopper, KR Adams, BJ AF Chaston, John M. Dillman, Adler R. Shapiro-Ilan, David I. Bilgrami, Anwar L. Gaugler, Randy Hopper, Keith R. Adams, Byron J. TI Outcrossing and crossbreeding recovers deteriorated traits in laboratory cultured Steinernema carpocapsae nematodes SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Biological control; Entomopathogenic nematodes; Inbreeding depression; Steinernema carpocapsae; Trait deterioration ID ENTOMOPATHOGENIC NEMATODES; HETERORHABDITIS-BACTERIOPHORA; XENORHABDUS-NEMATOPHILA; INBREEDING DEPRESSION; HOST; COLONIZATION; GENETICS; LARVAE AB The nematode Steinernema carpocapsae infects and kills many pest insects in agro-ecosystems and is commonly used in biocontrol of these pests. Growth of the nematodes prior to distribution for biocontrol commonly results in deterioration of traits that are essential for nematode persistence in field applications. To better understand the mechanisms underlying trait deterioration of the efficacy of natural parasitism in entomopathogenic nematodes, we explored the maintenance of fitness related traits including reproductive capacity, heat tolerance, virulence to insects and 'tail standing' (formerly called nictation) among laboratory-cultured lines derived from natural, randomly mating populations of S. carpocapsae. Laboratory cultured nematode lines with fitness-related trait values below wild-type levels regained wild-type levels of reproductive and heat tolerance traits when outcrossed with a non-deteriorated line, while virulence and 'tail standing' did not deteriorate in our experiments. Crossbreeding two trait-deteriorated lines with each other also resulted in restoration of trait means to wild-type levels in most crossbred lines. Our results implicate inbreeding depression as the primary cause of trait deterioration in the laboratory cultured S. carpocapsae. We further suggest the possibility of creating inbred lines purged of deleterious alleles as founders in commercial nematode growth. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. C1 [Adams, Byron J.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Chaston, John M.; Dillman, Adler R.] Brigham Young Univ, Microbiol & Mol Biol Dept, Provo, UT 84602 USA. [Chaston, John M.; Dillman, Adler R.; Adams, Byron J.] Brigham Young Univ, Evolutionary Ecol Lab, Provo, UT 84602 USA. [Shapiro-Ilan, David I.] ARS, SE Fruit & Tree Nut Res Unit, USDA, Byron, GA 31008 USA. [Bilgrami, Anwar L.; Gaugler, Randy] Rutgers State Univ, Ctr Vector Biol, New Brunswick, NJ 08901 USA. [Hopper, Keith R.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. RP Adams, BJ (reprint author), Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. EM bjadams@byu.edu RI Adams, Byron/C-3808-2009; Gaugler, Randy/K-3898-2013; OI Adams, Byron/0000-0002-7815-3352; Dillman, Adler/0000-0001-7171-4332 FU National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service [2002-01974]; Brigham Young University (USA); National Institutes of Health (USA) National Research Service [AI55397]; National Science Foundation; Brigham Young University Office of Creative Research and Activities Mentoring FX This study was supported in part by the National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service, Grant No. 2002-01974 to DIS-I, RG and BJA, and a Brigham Young University (USA) Mentored Environment Grant to BJA. JMC was supported by a National Institutes of Health (USA) National Research Service Award AI55397 in "Microbes in Health and Disease", and a National Science Foundation Graduate Research Fellowship. ARD and JMC were supported by a Brigham Young University Office of Creative Research and Activities Mentoring Grant. We thank members of the Nematode Evolution Laboratory at Brigham Young University for helpful discussion, Kathy Halat for technical assistance, and Robert Zimmerman for encouragement and inspiration. NR 31 TC 9 Z9 9 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 J9 INT J PARASITOL JI Int. J. Parasit. PD JUN PY 2011 VL 41 IS 7 BP 801 EP 809 DI 10.1016/j.ijpara.2011.02.005 PG 9 WC Parasitology SC Parasitology GA 774IA UT WOS:000291377700012 PM 21447341 ER PT J AU Lee, KW Lillehoj, HS Jang, SI Li, GX Bautista, DA Phillips, K Ritter, D Lillehoj, EP Siragusa, GR AF Lee, K. W. Lillehoj, H. S. Jang, S. I. Li, G. X. Bautista, D. A. Phillips, K. Ritter, D. Lillehoj, E. P. Siragusa, G. R. TI Effects of coccidiosis control programs on antibody levels against selected pathogens and serum nitric oxide levels in broiler chickens SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE chicken anemia virus; Clostridium; coccidiosis control program; infectious bursal disease virus; reovirus ID CLOSTRIDIUM-PERFRINGENS; NECROTIC ENTERITIS; BACTERIAL COMMUNITY; EIMERIA-TENELLA; ALPHA-TOXIN; PREVALENCE; SUSCEPTIBILITY; MACROPHAGES; DISEASE; SALINOMYCIN AB In-feed antibiotic growth promoters (AGP) are used in commercial poultry production to improve performance and health. In addition, anticoccidial drugs and vaccines provide protection against avian coccidiosis. However, the combined effects of AGP and anticoccidial control programs on poultry health and immune status have not been adequately addressed. Therefore, we assessed live coccidia vaccination, or feeding with 1 of 6 different diets supplemented with coccidiostatic drugs, in combination with AGP, on antibody levels against selected pathogens and serum nitric oxide (NO) levels in broilers. One-day-old untreated and treated chickens were raised on used litter from a gangrenous dermatitis-endemic farm as a source of microbial infection. Clostridium infection in untreated birds was confirmed by the appearance of bacteria-reactive antibodies, with the early presence of antibodies against chicken anemia virus and infectious bursal disease virus and decreasing levels of NO. Coccidia-vaccinated birds given AGP-supplemented diets, in general, had Clostridium antibody levels equal to those of untreated controls. Similarly, few differences between control and vaccinated groups were evident with seropositivity or NO levels. By contrast, most of the coccidiostat plus AGP treatment groups were associated with decreased Clostridium antibody levels compared not only with the unmedicated group, but also with the coccidia vaccine plus AGP group. We conclude that the use of anticoccidial drugs plus AGP may reduce the incidence of Clostridium infection in poultry compared with coccidia vaccination in the presence of AGP, at least under the experimental test conditions used in this study. C1 [Lee, K. W.; Lillehoj, H. S.; Jang, S. I.; Li, G. X.] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Bautista, D. A.; Phillips, K.] Univ Delaware, Lasher Poultry Diagnost Lab, Georgetown 16483, Guyana. [Ritter, D.] Mountaire Farms Inc, Millsboro, DE 19966 USA. [Lillehoj, E. P.] Univ Maryland, Dept Pediat, Sch Med, Baltimore, MD 21201 USA. [Siragusa, G. R.] Danisco, Waukesha, WI 53186 USA. RP Lillehoj, HS (reprint author), ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov OI Lee, Kyung-Woo/0000-0002-3533-7979 FU USDA Agricultural Research Service and Danisco [1265-32000-086-00D] FX This project was supported by a Trust agreement established between USDA Agricultural Research Service and Danisco and partially by the Agricultural Research Service in-house project 1265-32000-086-00D. We thank Marjorie Nichols and Stacy Torreyson in the Animal Parasitic Diseases Laboratory (Beltsville, MD) for technical assistance. NR 40 TC 4 Z9 4 U1 1 U2 5 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2011 VL 20 IS 2 BP 143 EP 152 DI 10.3382/japr.2010-00218 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 770OY UT WOS:000291098900005 ER PT J AU Barker, KJ Coufal, CD Purswell, JL Davis, JD Parker, HM Kidd, MT McDaniel, CD Kiess, AS AF Barker, K. J. Coufal, C. D. Purswell, J. L. Davis, J. D. Parker, H. M. Kidd, M. T. McDaniel, C. D. Kiess, A. S. TI In-house windrowing of a commercial broiler farm during the summer months and its effect on litter composition SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler; windrowing; ammonia; temperature; microbe ID POULTRY LITTER; PATHOGENS; AMMONIA; CHAMBER; FLUX AB In-house windrowing of broiler litter between flocks has been suggested as an effective method to reduce pathogens and improve the quality of the house environment in which new chicks are placed. In this study, different methods of in-house windrowing were tested in commercial broiler houses during the summer months of July and August to determine the effects each would have on litter composition. Three windrowing treatments and 1 control treatment were applied to 2 commercial broiler houses containing litter that was used over multiple flock grow-outs. Windrowing treatments included windrows with either the addition of water, turning of the windrow, or leaving the windrow undisturbed for 8 d. Each commercial broiler house was divided into 16 plots, each 6.1 x 6.1 m, with each treatment being applied to 4 plots per house. Litter from each plot was analyzed for moisture content, pH, NH(3), and microbes over a 17-d period, with d 17 representing 7 d after chick placement. Moisture content was significantly lower in the control treatment compared with all other treatments. Litter in all treatments had decreased pH from d 0 to 17. Ammonia decreased from d 10 to 17. Litter windrowed without the addition of water or turning resulted in a decrease in anaerobic bacteria on d 17 compared with the control treatment. In conclusion, in-house windrowing may be beneficial for newly placed chicks by changing the litter environment and reducing the population of pathogenic anaerobes, which can be detrimental to bird health and production. C1 [Barker, K. J.; Parker, H. M.; McDaniel, C. D.; Kiess, A. S.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. [Coufal, C. D.] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. [Purswell, J. L.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. [Davis, J. D.] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Kidd, M. T.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. RP Kiess, AS (reprint author), Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. EM akiess@poultry.msstate.edu NR 26 TC 3 Z9 3 U1 1 U2 4 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2011 VL 20 IS 2 BP 168 EP 180 DI 10.3382/japr.2010-00242 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA 770OY UT WOS:000291098900008 ER PT J AU Berrang, ME Smith, DP Meinersmann, RJ AF Berrang, M. E. Smith, D. P. Meinersmann, R. J. TI Variations on standard broiler processing in an effort to reduce Campylobacter numbers on postpick carcasses SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler processing; Campylobacter; defeathering; feather picking ID CROSS-CONTAMINATION; SALMONELLA; PREVALENCE; RECOVERY; CHICKEN; COUNTS; LEVEL; SPP.; INFECTION; TURKEY AB Campylobacter numbers increase on broiler carcasses during defeathering because of leakage of gut contents through the vent. We tested several processing modifications designed to interfere with the transfer of Campylobacter from gut contents to the carcass surface. The numbers of Campylobacter detected on the breast skin of carcasses treated with each modification was compared with the numbers on control broilers processed using a standard method. Filling the vent and colon with commercially available canned spray foam did not consistently form an effective plug, and Campylobacter numbers increased during picking. Likewise, hanging carcasses with the vent pointed downward during defeathering was not effective in preventing the increase in Campylobacter numbers. Eviscerating carcasses by hand immediately before defeathering eliminated the increase in Campylobacter during automated feather picking. However, inadvertent contamination during hand evisceration led to higher numbers before feather removal. Therefore, we tested hand evisceration before scalding, allowing the scald water to kill and wash away Campylobacter spilled on the carcass during evisceration. Prescald evisceration was effective in significantly moderating the increase in Campylobacter on broiler carcasses during automated defeathering. Changing the order of standard broiler processing may help to control contamination with Campylobacter. C1 [Berrang, M. E.; Meinersmann, R. J.] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. [Smith, D. P.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC 27695 USA. RP Berrang, ME (reprint author), ARS, USDA, Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM berrang@ars.usda.gov NR 25 TC 1 Z9 1 U1 0 U2 2 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2011 VL 20 IS 2 BP 197 EP 202 DI 10.3382/japr.2010-00274 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 770OY UT WOS:000291098900011 ER PT J AU Aldini, G Orioli, M Rossoni, G Savi, F Braidotti, P Vistoli, G Yeum, KJ Negrisoli, G Carini, M AF Aldini, Giancarlo Orioli, Marica Rossoni, Giuseppe Savi, Federica Braidotti, Paola Vistoli, Giulio Yeum, Kyung-Jin Negrisoli, Gianpaolo Carini, Marina TI The carbonyl scavenger carnosine ameliorates dyslipidaemia and renal function in Zucker obese rats SO JOURNAL OF CELLULAR AND MOLECULAR MEDICINE LA English DT Article DE carnosine; L and D enantiomers; carbonyl quenching activity; metabolic syndrome; obese Zucker rats; chronic treatment; renoprotection ID HISTIDINE-CONTAINING PEPTIDES; TANDEM MASS-SPECTROMETRY; METABOLIC SYNDROME-X; LIPID-PEROXIDATION; OXIDATIVE STRESS; PROTEIN CARBONYLATION; SPECTROPHOTOMETRIC METHOD; CELLULAR DYSFUNCTION; MEDIATED INHIBITION; SYMPATHETIC-NERVE AB The metabolic syndrome is a risk factor that increases the risk for development of renal and vascular complications. This study addresses the effects of chronic administration of the endogenous dipeptide carnosine (beta-alanyl-L-histidine, L-CAR) and of its enantiomer (beta-alanyl-D-histidine, D-CAR) on hyperlipidaemia, hypertension, advanced glycation end products, advanced lipoxidation end products formation and development of nephropathy in the non-diabetic, Zucker obese rat. The Zucker rats received a daily dose of L-CAR or D-CAR (30 mg/kg in drinking water) for 24 weeks. Systolic blood pressure was recorded monthly. At the end of the treatment, plasma levels of triglycerides, total cholesterol, glucose, insulin, creatinine and urinary levels of total protein, albumin and creatinine were measured. Several indices of oxidative/carbonyl stress were also measured in plasma, urine and renal tissue. We found that both L- and D-CAR greatly reduced obese-related diseases in obese Zucker rat, by significantly restraining the development of dyslipidaemia, hypertension and renal injury, as demonstrated by both urinary parameters and electron microscopy examinations of renal tissue. Because the protective effect elicited by L- and D-CAR was almost superimposable, we conclude that the pharmacological action of L-CAR is not due to a pro-histaminic effect (D-CAR is not a precursor of histidine, since it is stable to peptidic hydrolysis), and prompted us to propose that some of the biological effects can be mediated by a direct carbonyl quenching mechanism. C1 [Aldini, Giancarlo; Orioli, Marica; Vistoli, Giulio; Carini, Marina] Univ Milan, Dept Pharmaceut Sci Pietro Pratesi, I-20133 Milan, Italy. [Rossoni, Giuseppe] Univ Milan, Dept Pharmacol Chemotherapy & Med Toxicol, I-20133 Milan, Italy. [Savi, Federica; Braidotti, Paola] San Paolo Hosp, Dept Med Surg & Odontol, Milan, Italy. [Savi, Federica; Braidotti, Paola] Univ Milan, IRCCS Fdn Policlin, I-20133 Milan, Italy. [Yeum, Kyung-Jin] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Negrisoli, Gianpaolo] Flamma SpA, Chignolo Disol, Prov Bergamo, Italy. RP Aldini, G (reprint author), Univ Milan, Dept Pharmaceut Sci Pietro Pratesi, Via Mangiagalli 25, I-20133 Milan, Italy. EM giancarlo.aldini@unimi.it RI orioli, marica/F-7606-2015; CARINI, MARINA/D-3084-2015; OI orioli, marica/0000-0003-1558-9551; CARINI, MARINA/0000-0003-3407-5425; aldini, giancarlo/0000-0002-2355-6744; Vistoli, Giulio/0000-0002-3939-5172 FU University of Milan; Regione Lombardia-MIUR [L.297 - Art. 12/BioTech DM27909] FX This work was supported by funds from the University of Milan (PUR 2007, 2008) MIUR (PRIN 2007) and from Regione Lombardia-MIUR (L.297 - Art. 12/BioTech DM27909). The authors are grateful to Jaclyn Michelle Winkel for English language corrections. NR 73 TC 57 Z9 60 U1 2 U2 9 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1582-1838 J9 J CELL MOL MED JI J. Cell. Mol. Med. PD JUN PY 2011 VL 15 IS 6 BP 1339 EP 1354 DI 10.1111/j.1582-4934.2010.01101.x PG 16 WC Cell Biology; Medicine, Research & Experimental SC Cell Biology; Research & Experimental Medicine GA 769ZX UT WOS:000291057900010 PM 20518851 ER PT J AU Hallacoglu, B Sassaroli, A Fantini, S Troen, AM AF Hallacoglu, Bertan Sassaroli, Angelo Fantini, Sergio Troen, Aron M. TI Cerebral perfusion and oxygenation are impaired by folate deficiency in rat: absolute measurements with noninvasive near-infrared spectroscopy SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE aging; capillaries; CBF; NIRS; nutrition; VCI ID VASCULAR COGNITIVE IMPAIRMENT; ALZHEIMERS-DISEASE; BRAIN OXYGENATION; TOTAL HOMOCYSTEINE; SCATTERING MEDIA; LIGHT-SCATTERING; FREQUENCY-DOMAIN; BLOOD-FLOW; HYPERHOMOCYSTEINEMIA; HEMOGLOBIN AB Brain microvascular pathology is a common finding in Alzheimer's disease and other dementias. However, the extent to which microvascular abnormalities cause or contribute to cognitive impairment is unclear. Noninvasive near-infrared spectroscopy (NIRS) can address this question, but its use for clarifying the role of microvascular dysfunction in dementia has been limited due to theoretical and practical considerations. We developed a new noninvasive NIRS method to obtain quantitative, dynamic measurements of absolute brain hemoglobin concentration and oxygen saturation and used it to show significant cerebrovascular impairments in a rat model of diet-induced vascular cognitive impairment. We fed young rats folate-deficient (FD) and control diets and measured absolute brain hemoglobin and hemodynamic parameters at rest and during transient mild hypoxia and hypercapnia. With respect to control animals, FD rats featured significantly lower brain hemoglobin concentration (72 +/- 4 mu mol/L versus 95 +/- 6 mu mol/L) and oxygen saturation (54% +/- 3% versus 65% +/- 2%). By contrast, resting arterial oxygen saturation was the same for both groups (96% +/- 4%), indicating that decrements in brain hemoglobin oxygenation were independent of blood oxygen carrying capacity. Vasomotor reactivity in response to hypercapnia was also impaired in FD rats. Our results implicate microvascular abnormality and diminished oxygen delivery as a mechanism of cognitive impairment. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 1482-1492; doi:10.1038/jcbfm.2011.13; published online 9 March 2011 C1 [Troen, Aron M.] Hebrew Univ Jerusalem, Nutr & Brain Hlth Lab, Inst Biochem Food Sci & Nutr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel. [Hallacoglu, Bertan; Sassaroli, Angelo; Fantini, Sergio] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA. [Troen, Aron M.] Tufts Univ, Nutr & Neurocognit Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Troen, AM (reprint author), Hebrew Univ Jerusalem, Nutr & Brain Hlth Lab, Inst Biochem Food Sci & Nutr, Robert H Smith Fac Agr Food & Environm, POB 12, IL-76100 Rehovot, Israel. EM troen@agri.huji.ac.il RI Hallacoglu, Bertan/K-4175-2012 FU Unilever USA; Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts; US Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This work is supported by a strategic research agreement between Unilever USA and the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts, and by the US Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. NR 44 TC 13 Z9 14 U1 0 U2 6 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD JUN PY 2011 VL 31 IS 6 BP 1482 EP 1492 DI 10.1038/jcbfm.2011.13 PG 11 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA 771HY UT WOS:000291149000018 PM 21386853 ER PT J AU Pedersen, TL Keyes, WR Shahab-Ferdows, S Allen, LH Newman, JW AF Pedersen, Theresa L. Keyes, William R. Shahab-Ferdows, Setareh Allen, Lindsay H. Newman, John W. TI Methylmalonic acid quantification in low serum volumes by UPLC-MS/MS SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE Methylmalonic acid; Vitamin B12 status; UPLC; LC-MS/MS; Nutrition ID TANDEM MASS-SPECTROMETRY; TOTAL HOMOCYSTEINE; ISOTOPE DILUTION; PLASMA; URINE; INDICATORS; DEFICIENCY; MARKERS AB Methylmalonic acid (MMA) is a metabolic intermediate transformed to succinic acid (SA) by a vitamin B-12-dependent catalytic step, and is broadly used as a clinical biomarker of functional vitamin B12 status. However, reported methods use between 100 and 1000 mu L of serum or plasma making them sub-optimal for sample-limited studies, including those with neonates and infants. LC-MS/MS based protocols to measure MMA as n-butyl esters in the presence of tri-deuterated MMA (MMA-d(3)) were modified for use with 25 mu L of human serum by scaling down sample processing volumes and analysis by UPLC-MS/MS. Plasma-based calibration solutions were found to be unnecessary, and chromatographic resolution and peak shape of SA and MMA was optimized in <4 min with isocratic 53:47 methanol/1.67 mM (pH 6.5) ammonium formate. Additionally, 1-cyclohexyl-urido-3-dodecanoic acid (CUDA) was included as internal standard allowing direct assessment of MMA recovery. Sample concentrations in the low normal range produced a signal:noise of >100:1. MMA intra- and inter-assay variability was under 10%. MMA-d(3) surrogate recovery averaged 93 +/- 14%. MMA stability exceeded three years in frozen samples and was unaffected by up to five freeze/thaw cycles. In conclusion, we report that methylmalonic acid can be measured with 25 mu L. of serum using water based standards. The assay signal:noise per concentration indicates that the method could perform as implemented with as little as 5 mu L, of serum. The reported method is applicable for studies of functional B12 status in sample limited experiments including investigations of nutritional status in neonates and in studies where low normal MMA levels are expected. Published by Elsevier B.V. C1 [Pedersen, Theresa L.; Keyes, William R.; Shahab-Ferdows, Setareh; Allen, Lindsay H.; Newman, John W.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Newman, JW (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM theresa.pedersen@ars.usda.gov; william.keyes@ars.usda.gov; setareh.shahab-ferdows@ars.usda.gov; lindsay.allen@ars.usda.gov; john.newman@ars.usda.gov FU USDA [5306-5152-006-00D, 5306-5152-007-00D, 5306-51530-016-00D, 5306-51530-019-00D]; Bristol-Meyers Squibb Company [010262] FX This project was supported by in part by USDA projects 5306-5152-006-00D 5306-5152-007-00D, 5306-51530-016-00D, 5306-51530-019-00D, and the Bristol-Meyers Squibb Company research grant "Promoting New Directions in International Nutrition Research at UC Davis (UCD OVCR#: 010262). The USDA is an equal opportunity provider and employer. NR 19 TC 12 Z9 12 U1 0 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 J9 J CHROMATOGR B JI J. Chromatogr. B PD JUN 1 PY 2011 VL 879 IS 19 BP 1502 EP 1506 DI 10.1016/j.jchromb.2011.03.039 PG 5 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 773SL UT WOS:000291330600002 PM 21497144 ER PT J AU Greenberg, AS Coleman, RA Kraemer, FB McManaman, JL Obin, MS Puri, V Yan, QW Miyoshi, H Mashek, DG AF Greenberg, Andrew S. Coleman, Rosalind A. Kraemer, Fredric B. McManaman, James L. Obin, Martin S. Puri, Vishwajeet Yan, Qing-Wu Miyoshi, Hideaki Mashek, Douglas G. TI The role of lipid droplets in metabolic disease in rodents and humans SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Review ID DIFFERENTIATION-RELATED PROTEIN; ADIPOSE TRIGLYCERIDE LIPASE; FATTY LIVER-DISEASE; HORMONE-SENSITIVE LIPASE; NECROSIS-FACTOR-ALPHA; DIET-INDUCED OBESITY; MAGNETIC-RESONANCE-SPECTROSCOPY; SEIP CONGENITAL LIPODYSTROPHY; INDUCED INSULIN-RESISTANCE; ACTIVATED-RECEPTOR-ALPHA AB Lipid droplets (LDs) are intracellular organelles that store neutral lipids within cells. Over the last two decades there has been a dramatic growth in our understanding of LD biology and, in parallel, our understanding of the role of LDs in health and disease. In its simplest form, the LD regulates the storage and hydrolysis of neutral lipids, including triacylglycerol and/or cholesterol esters. It is becoming increasingly evident that alterations in the regulation of LD physiology and metabolism influence the risk of developing metabolic diseases such as diabetes. In this review we provide an update on the role of LD-associated proteins and LDs in metabolic disease. C1 [Greenberg, Andrew S.; Obin, Martin S.; Yan, Qing-Wu] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Obes & Metab Lab, Boston, MA 02111 USA. [Coleman, Rosalind A.] Univ N Carolina, Dept Nutr, Chapel Hill, NC USA. [Kraemer, Fredric B.] Stanford Univ, Div Endocrinol, Palo Alto, CA 94304 USA. [Kraemer, Fredric B.] VA Palo Alto Hlth Care Syst, Palo Alto, CA USA. [McManaman, James L.] Univ Colorado, Dept Obstet & Gynecol, Div Reprod Sci, Denver, CO 80202 USA. [Puri, Vishwajeet] Boston Univ, Sch Med, Dept Med, Sect Endocrinol Diabet & Nutr, Boston, MA 02118 USA. [Miyoshi, Hideaki] Hokkaido Univ, Grad Sch Med, Dept Internal Med 2, Sapporo, Hokkaido, Japan. [Mashek, Douglas G.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN USA. RP Greenberg, AS (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Obes & Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM Andrew.greenberg@tufts.edu OI Mashek, Douglas/0000-0001-7033-3386 FU American Diabetes Association [7-08-RA-57, 7-07-JF-43]; USDA Agricultural Research Service [58-1950-7-707]; Department of Veterans Affairs Office of Research and Development Medical Research Service; NIH [AG028098, DK0822574, 1RC2ES018781, R24DK087669, DK56598, DK59935, RO1-HD045962, PO1-HD38129, 3P30DK046200, DK074979, DK085008]; Merck Co.; Life Sciences Institute of the University of Michigan FX This work was supported by grants from the American Diabetes Association (7-08-RA-57 to A.S. Greenberg; 7-07-JF-43 to D.G. Mashek), the USDA Agricultural Research Service (58-1950-7-707 to A.S. Greenberg), the Department of Veterans Affairs Office of Research and Development Medical Research Service (to F.B. Kraemer) and the NIH (AG028098 to F.B. Kraemer; DK0822574, 1RC2ES018781, and R24DK087669 to A.S. Greenberg; DK56598 and DK59935 to R.A. Coleman; RO1-HD045962 and PO1-HD38129 to J.L. McManaman; 3P30DK046200 to V. Puri; DK074979 to M.S. Obin; and DK085008 to D.G. Mashek). The Obesity Review Series is supported in part by unrestricted educational grants from Merck & Co. and the Life Sciences Institute of the University of Michigan. NR 155 TC 200 Z9 202 U1 8 U2 50 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JUN PY 2011 VL 121 IS 6 BP 2102 EP 2110 DI 10.1172/JCI46069 PG 9 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 772LB UT WOS:000291234300007 PM 21633178 ER PT J AU Edupuganti, S Rouphael, N Mehta, A Eaton, M Heller, JG Bressler, A Brandt, M O'Donnell, K AF Edupuganti, Srilatha Rouphael, Nadine Mehta, Aneesh Eaton, Molly Heller, John G. Bressler, Adam Brandt, Mary O'Donnell, Kerry TI Fusarium falciforme Vertebral Abscess and Osteomyelitis: Case Report and Molecular Classification SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SOLANI SPECIES COMPLEX; DNA-SEQUENCE DATABASE; ANTIFUNGAL SUSCEPTIBILITY; INFECTIONS; IDENTIFICATION; MANAGEMENT AB Fusarium is a ubiquitous mold that can cause superficial infections such as keratitis and onychomycosis in immunocompetent humans; however, infections in immunocompromised hosts can be fatal. We report an unusual case of epidural abscess and vertebral osteomyelitis in a patient with an autoimmune disorder who was on long-term glucocorticoids. Multilocus DNA sequence-based typing revealed that the infection was caused by a novel three-locus haplotype of Fusarium falciforme designated FSSC 3+4qqq. C1 [Edupuganti, Srilatha; Rouphael, Nadine; Mehta, Aneesh; Eaton, Molly] Emory Univ, Sch Med, Dept Med, Div Infect Dis, Atlanta, GA USA. [Heller, John G.] Emory Univ, Sch Med, Dept Orthopaed Surg, Atlanta, GA USA. [Bressler, Adam] Infect Dis Specialists Atlanta, Atlanta, GA USA. [Brandt, Mary] Ctr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA USA. [O'Donnell, Kerry] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Edupuganti, S (reprint author), Emory Univ, Sch Med, Div Infect Dis, Hope Clin,Emory Vaccine Ctr, 603 Church St, Decatur, GA 30030 USA. EM sedupug@emory.edu RI Mehta, Aneesh/B-8054-2012 OI Mehta, Aneesh/0000-0002-6552-9162 NR 21 TC 8 Z9 8 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUN PY 2011 VL 49 IS 6 BP 2350 EP 2353 DI 10.1128/JCM.02547-10 PG 4 WC Microbiology SC Microbiology GA 769NZ UT WOS:000291024500055 PM 21450957 ER PT J AU Hemashettar, BM Patil, RN O'Donnell, K Chaturvedi, V Ren, P Padhye, AA AF Hemashettar, B. M. Patil, R. N. O'Donnell, Kerry Chaturvedi, Vishnu Ren, Ping Padhye, Arvind A. TI Chronic Rhinofacial Mucormycosis Caused by Mucor irregularis (Rhizomucor variabilis) in India SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID ZYGOMYCOSIS; SPECTRUM; DISEASE AB In this article, we describe a chronic case of rhinofacial mucormycosis caused by Mucor irregularis, formerly known as Rhizomucor variabilis var. variabilis, a rare mycotic agent in humans. The infection caused progressive destruction of the nasal septum and soft and hard palate, leading to collapse of the nose bridge and an ulcerative gaping hole. The mucoralean mold cultured from a nasal biopsy specimen was determined by multilocus DNA sequence data to be conspecific with M. irregularis. C1 [O'Donnell, Kerry] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Hemashettar, B. M.] Hi Tech Hlth Care & Diagnost Ctr Pvt Ltd, Belgaum 590002, India. [Patil, R. N.] Jawaharlal Nehru Med Coll, Dept Ear Nose & Throat, Belgaum 590010, India. [Chaturvedi, Vishnu; Ren, Ping] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA. [Padhye, Arvind A.] Ctr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA 30333 USA. RP O'Donnell, K (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM kerry.odonnell@ars.usda.gov NR 23 TC 18 Z9 18 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUN PY 2011 VL 49 IS 6 BP 2372 EP 2375 DI 10.1128/JCM.02326-10 PG 4 WC Microbiology SC Microbiology GA 769NZ UT WOS:000291024500061 PM 21508154 ER PT J AU Vogel, RM Yaindl, C Walter, M AF Vogel, Richard M. Yaindl, Chad Walter, Meghan TI Nonstationarity: Flood Magnification and Recurrence Reduction Factors in the United States SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE flooding; runoff; land-use; land-cover change; urbanization; rivers; streams; trend analysis ID MANAGEMENT; TRENDS AB It may no longer be reasonable to model streamflow as a stationary process, yet nearly all existing water resource planning methods assume that historical streamflows will remain unchanged in the future. In the few instances when trends in extreme events have been considered, most recent work has focused on the influence of climate change, alone. This study takes a different approach by exploring trends in floods in watersheds which are subject to a very broad range of anthropogenic influences, not limited to climate change. A simple statistical model is developed which can both mimic observed flood trends as well as the frequency of floods in a nonstationary world. This model is used to explore a range of flood planning issues in a nonstationary world. A decadal flood magnification factor is defined as the ratio of the T-year flood in a decade to the T-year flood today. Using historical flood data across the United States we obtain flood magnification factors in excess of 2-5 for many regions of the United States, particularly those regions with higher population densities. Similarly, we compute recurrence reduction factors which indicate that what is now considered the 100-year flood, may become much more common in many watersheds. Nonstationarity in floods can result from a variety of anthropogenic processes including changes in land use, climate, and water use, with likely interactions among those processes making it very difficult to attribute trends to a particular cause. C1 [Vogel, Richard M.] Tufts Univ, Dept Civil & Environm Engn, Medford, MA 02155 USA. [Yaindl, Chad] GeoSyntec, Acton, MA 01720 USA. [Walter, Meghan] Nat Resources Conservat Serv, USDA, Portland, OR 97232 USA. RP Vogel, RM (reprint author), Tufts Univ, Dept Civil & Environm Engn, Medford, MA 02155 USA. EM richard.vogel@tufts.edu RI Vogel, Richard/A-8513-2008 OI Vogel, Richard/0000-0001-9759-0024 NR 12 TC 63 Z9 68 U1 2 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2011 VL 47 IS 3 BP 464 EP 474 DI 10.1111/j.1752-1688.2011.00541.x PG 11 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 772HL UT WOS:000291224900004 ER PT J AU Allan, SA AF Allan, Sandra A. TI Susceptibility of adult mosquitoes to insecticides in aqueous sucrose baits SO JOURNAL OF VECTOR ECOLOGY LA English DT Article DE Mosquito; insecticide; sugar bait; Culex; Aedes; Anopheles ID LARVICIDE BACILLUS-SPHAERICUS; ANOPHELES-GAMBIAE; AEDES-AEGYPTI; NEONICOTINOID INSECTICIDE; LABORATORY EVALUATION; PYRETHROID-RESISTANT; FLORIDA MOSQUITOS; FIELD-EVALUATION; MUSCA-DOMESTICA; DIFFERENT MODES AB Mosquitoes characteristically feed on plant-derived carbohydrates and honeydew just after emergence and intermittently during their lives. Development of toxic baits focusing on this carbohydrate-seeking behavior may potentially contribute to localized control. In the present study, ten insecticides were fed to female Culex quinquefasciatus, Anopheles quadrimaculatus, and Aedes taeniorhynchus in a 10% sucrose solution. Active ingredients representative of five classes of insecticides (pyrethroids, phenylpyroles, pyrroles, neonicotinoids, and macrocyclic lactones) were selected for comparison with commercial formulations used to facilitate incorporation of active ingredients into aqueous sucrose solutions. Sucrose as a phagostimulant significantly enhanced mortality to toxicants. In general, the most effective active ingredients were fipronil, deltamethrin and imidacloprid, followed by spinosad, thiamethoxam, bifenthrin, permethrin, and cyfluthrin. The least effective ingredients were chlorfenapyr and ivermectin. For some of the ingredients tested, Cx. quinquefasciatus was the least susceptible species. One-day-old male Cx. quinquefasciatus were more susceptible than females; however, no differences existed between one-and seven-day-old mosquitoes. There were no differences in susceptibility between unfed and gravid ten-day-old female Cx. quinquefasciatus to bifenthrin. In conclusion, several pesticides from different classes of compounds have potential for use in development of toxic baits for mosquitoes. Journal of Vector Ecology 36 (1): 59-67. 2011. C1 ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. RP Allan, SA (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. FU U.S. Department of Defense through the Armed Forces Pest Management Board FX Thanks are extended to E. Vrzal and C. Brown for technical assistance, H. Brown for providing mosquitoes, and C. Geden, J. Cilek, and L. Cohnstaedt for critical review of the manuscript. This study was supported by a Deployed War-Fighter Protection Research Program Grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. The use of trade, firm, or corporation names in this publication are for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 48 TC 17 Z9 17 U1 2 U2 14 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 J9 J VECTOR ECOL JI J. Vector Ecol. PD JUN PY 2011 VL 36 IS 1 BP 59 EP 67 PG 9 WC Entomology SC Entomology GA 772II UT WOS:000291227200008 PM 21635642 ER PT J AU Li, G Sarazin, P Orts, WJ Imam, SH Favis, BD AF Li, Gang Sarazin, Pierre Orts, William J. Imam, Syed H. Favis, Basil D. TI Biodegradation of Thermoplastic Starch and its Blends with Poly(lactic acid) and Polyethylene: Influence of Morphology SO MACROMOLECULAR CHEMISTRY AND PHYSICS LA English DT Article DE biodegradation; morphology; polyethylene; poly(lactic acid); thermoplastic starch ID POLYCAPROLACTONE; DEGRADATION; INVERSION AB The room temperature mineralization of thermoplastic starch (TPS) with a high glycerol content and its blends with low-density polyethylene (LDPE) and polylactic acid (PLA) are examined under controlled degradation conditions. These results are correlated with the morphologies and continuity behavior of the various blend systems. It is found that thermoplastic starch degrades more rapidly than native starch. Lowering the glycerol content in the TPS has virtually no effect on its biodegradation behavior. The only contribution to biodegradation of the TPS blend is from the TPS component. Blending TPS with LDPE and PLA in a co-continuous morphology at a 50/50 composition provides a significant increase in TPS surface area, which increases the biodegradation rate for the blends as compared to pure TPS. The results indicate a close relationship between morphology, phase continuity, and biodegradation behavior. C1 [Li, Gang; Sarazin, Pierre; Favis, Basil D.] Ecole Polytech Montreal, CREPEC, Dept Chem Engn, Montreal, PQ H3C 3A7, Canada. [Orts, William J.; Imam, Syed H.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Favis, BD (reprint author), Ecole Polytech Montreal, CREPEC, Dept Chem Engn, POB 6079,Stn Ctr Ville, Montreal, PQ H3C 3A7, Canada. EM basil.favis@polymtl.ca NR 30 TC 9 Z9 10 U1 6 U2 36 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 1022-1352 J9 MACROMOL CHEM PHYS JI Macromol. Chem. Phys. PD JUN 1 PY 2011 VL 212 IS 11 BP 1147 EP 1154 DI 10.1002/macp.201100090 PG 8 WC Polymer Science SC Polymer Science GA 770TE UT WOS:000291110600006 ER PT J AU Al-Dous, EK George, B Al-Mahmoud, ME Al-Jaber, MY Wang, H Salameh, YM Al-Azwani, EK Chaluvadi, S Pontaroli, AC DeBarry, J Arondel, V Ohlrogge, J Saie, IJ Suliman-Elmeer, KM Bennetzen, JL Kruegger, RR Malek, JA AF Al-Dous, Eman K. George, Binu Al-Mahmoud, Maryam E. Al-Jaber, Moneera Y. Wang, Hao Salameh, Yasmeen M. Al-Azwani, Eman K. Chaluvadi, Srinivasa Pontaroli, Ana C. DeBarry, Jeremy Arondel, Vincent Ohlrogge, John Saie, Imad J. Suliman-Elmeer, Khaled M. Bennetzen, Jeffrey L. Kruegger, Robert R. Malek, Joel A. TI De novo genome sequencing and comparative genomics of date palm (Phoenix dactylifera) SO NATURE BIOTECHNOLOGY LA English DT Article ID LTR RETROTRANSPOSONS; ELAEIS-GUINEENSIS; SEX DETERMINATION; SHORT-READ; RICE; DIVERGENCE; EVOLUTION; ALIGNMENT; GENES; TOOL AB Date palm is one of the most economically important woody crops cultivated in the Middle East and North Africa and is a good candidate for improving agricultural yields in arid environments. Nonetheless, long generation times (5-8 years) and dioecy (separate male and female trees) have complicated its cultivation and genetic analysis. To address these issues, we assembled a draft genome for a Khalas variety female date palm, the first publicly available resource of its type for a member of the order Arecales. The similar to 380 Mb sequence, spanning mainly gene-rich regions, includes >25,000 gene models and is predicted to cover similar to 90% of genes and similar to 60% of the genome. Sequencing of eight other cultivars, including females of the Deglet Noor and Medjool varieties and their backcrossed males, identified >3.5 million polymorphic sites, including >10,000 genic copy number variations. A small subset of these polymorphisms can distinguish multiple varieties. We identified a region of the genome linked to gender and found evidence that date palm employs an XY system of gender inheritance. C1 Weill Cornell Med Coll Qatar, Dept Med Genet, Doha, Qatar. [Wang, Hao; Chaluvadi, Srinivasa; Pontaroli, Ana C.; DeBarry, Jeremy; Bennetzen, Jeffrey L.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Arondel, Vincent] Univ V Segalen Bordeaux, CNRS UMR, Lab Biogenese Membranaire, Bordeaux, France. [Ohlrogge, John] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Suliman-Elmeer, Khaled M.] Minist Environm, Ctr Biotechnol, Doha, Qatar. [Kruegger, Robert R.] Univ Calif Riverside, USDA ARS Natl Clonal Germplasm Repository Citrus, Riverside, CA 92521 USA. RP Malek, JA (reprint author), Weill Cornell Med Coll Qatar, Dept Med Genet, Doha, Qatar. EM jom2042@qatar-med.cornell.edu RI Chaluvadi, Srinivasa/C-7163-2011; Wang, Hao/C-7651-2009; OI Wang, Hao/0000-0002-9634-8778; DeBarry, Jeremy/0000-0001-5718-2675 FU Qatar Foundation FX This work was supported by Qatar Foundation Biomedical Research Program Grant funding. We thank G. Parra for running the CEGMA pipeline on our initial assembly. We thank the Cornell Center for Academic Computing for providing hardware systems support during the assembly stage. We thank K. Machaca and R. Crystal for critical review of the manuscript. NR 46 TC 142 Z9 149 U1 4 U2 47 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1087-0156 EI 1546-1696 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD JUN PY 2011 VL 29 IS 6 BP 521 EP U84 DI 10.1038/nbt.1860 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 773WL UT WOS:000291342100018 PM 21623354 ER PT J AU Zhao, L Lee, JY Hwang, DH AF Zhao, Ling Lee, Joo Y. Hwang, Daniel H. TI Inhibition of pattern recognition receptor-mediated inflammation by bioactive phytochemicals SO NUTRITION REVIEWS LA English DT Review DE phytochemical; pattern recognition receptor; inflammation; chronic disease ID TOLL-LIKE RECEPTOR; NF-KAPPA-B; NONPARENCHYMAL LIVER-CELLS; INDUCED INSULIN-RESISTANCE; LOW-DENSITY-LIPOPROTEIN; HELICOBACTER-PYLORI; SIGNALING PATHWAYS; IMMUNE-RESPONSES; INNATE IMMUNITY; FATTY-ACIDS AB Emerging evidence reveals that pattern-recognition receptors (PRRs), Toll-like receptors (TLRs), and nucleotide-binding oligomerization domain proteins (NODs) mediate both infection-induced and sterile inflammation by recognizing pathogen-associated molecular patterns and endogenous molecules, respectively. PRR-mediated chronic inflammation is a determinant for the development and progression of chronic diseases including cancer, atherosclerosis, and insulin resistance. Recent studies demonstrated that certain phytochemicals inhibit PRR-mediated pro-inflammation. Curcumin, helenalin, and cinnamaldehyde with alpha, beta-unsaturated carbonyl groups, or sulforaphane with an isothiocyanate group, inhibit TLR4 activation by interfering with cysteine residue-mediated receptor dimerization, while resveratrol, with no unsaturated carbonyl group, did not. Similarly, curcumin, parthenolide, and helenalin, but not resveratrol and (-)-epigallocatechin-3-gallate (EGCG), also inhibit NOD2 activation by interfering with NOD2 dimerization. In contrast, resveratrol, EGCG, luteolin, and structural analogs of luteolin specifically inhibit TLR3 and TLR4 signaling by targeting TANK binding kinase 1 (TBK1) and receptor interacting protein 1 (RIP1) in Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF) complex. Together, these results suggest that PRRs and downstream signaling components are molecular targets for dietary strategies to reduce PRR-mediated chronic inflammation and consequent risks of chronic diseases. C1 [Hwang, Daniel H.] Western Human Nutr Res Ctr, USDA, ARS, Davis, CA 95616 USA. [Zhao, Ling] Univ Tennessee, Dept Nutr, Knoxville, TN 37996 USA. [Lee, Joo Y.] Gwangju Inst Sci & Technol, Sch Life Sci, Kwangju, South Korea. [Hwang, Daniel H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Hwang, DH (reprint author), Western Human Nutr Res Ctr, USDA, ARS, Davis, CA 95616 USA. EM daniel.hwang@ars.usda.gov FU National Institutes of Health [DK064007, DK41868, CA75613]; United States Department of Agriculture (USDA) [2001-35200-10721]; American Institutes for Cancer Research [01A095Rev]; Western Human Nutrition Research Center/ARS/USDA; Korean Government (MEST) [20110000171]; Cell Dynamics Research Center, NRF, MEST [2010-0001617, 2011-0001156] FX This work was supported by grants DK064007, DK41868, and CA75613 from the National Institutes of Health, grant 2001-35200-10721 from the United States Department of Agriculture (USDA), grant 01A095Rev from the American Institutes for Cancer Research, and program funds from the Western Human Nutrition Research Center/ARS/USDA (D. H. H.) and grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (MEST) (20110000171) and Cell Dynamics Research Center, NRF, MEST (2010-0001617, 2011-0001156) (J.Y.L.). NR 86 TC 42 Z9 44 U1 1 U2 22 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD JUN PY 2011 VL 69 IS 6 BP 310 EP 320 DI 10.1111/j.1753-4887.2011.00394.x PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 772GR UT WOS:000291222800002 PM 21631512 ER PT J AU Hayes, RJ Wu, BM Subbarao, KV AF Hayes, Ryan J. Wu, BoMing Subbarao, Krishna V. TI A single recessive gene conferring short leaves in romaine x Latin type lettuce (Lactuca sativa L.) crosses, and its effect on plant morphology and resistance to lettuce drop caused by Sclerotinia minor Jagger SO PLANT BREEDING LA English DT Article DE genetics; breeding; cultivars; disease resistance; crop evolution ID PHENOTYPIC CHARACTERIZATION; POPULATION-STRUCTURE; MOSAIC-VIRUS AB P>Understanding the relationship between plant morphology and disease resistance is crucial to successful lettuce breeding. Latin type cultivars are a potential useful source of resistance to Sclerotinia minor for breeding resistant romaine cultivars. However, resistance in Latin cultivars may be conditioned by their relatively short stature. The objective of this research was to determine the segregation for plant height and the relationship between plant height and S. minor resistance in romaine x Latin crosses. The frequency of short plants was conditioned by a single recessive gene in F(1), F(2) and F(3) families from nine romaine x Latin crosses tested in 2007, 2008 and 2009 field experiments. The gene, named short leaf 1 (sl1), affects only leaf length in rosette plants and is morphologically distinct from other dwarfing genes. In two S. minor-infested field experiments with 75 F(3) romaine x 'Eruption' families, no difference in disease incidence was detected between short (sl1sl1), tall (Sl1Sl1) or segregating families. Therefore, development of resistant romaine cultivars from crosses with 'Eruption' is feasible. C1 [Hayes, Ryan J.] ARS, USDA, Salinas, CA 93905 USA. [Wu, BoMing] Oregon State Univ, Dept Bot & Plant Pathol, Cent Oregon Agr Res Ctr, Madras, OR USA. [Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, US Agr Res Stn, Salinas, CA USA. RP Hayes, RJ (reprint author), ARS, USDA, Salinas, CA 93905 USA. EM Ryan.Hayes@ars.usda.gov FU California Leafy Green Research Program FX This research was supported by the California Leafy Green Research Program. NR 21 TC 3 Z9 3 U1 0 U2 13 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2011 VL 130 IS 3 BP 388 EP 393 DI 10.1111/j.1439-0523.2010.01822.x PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 772HN UT WOS:000291225100013 ER PT J AU Jeong, Y Lee, S Choi, I Lim, Y Hur, Y Staub, JE Chung, S AF Jeong, YeSol Lee, SunYoung Choi, InHye Lim, YongPyo Hur, YoonKang Staub, Jack E. Chung, SangMin TI A method for selection of restriction enzymes for sdCAPS marker construction SO PLANT BREEDING LA English DT Article DE single-nucleotide polymorphism; sdCAPS; restriction enzyme selection AB P>Development of PCR-based markers for single-nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost-effective methodologies for SNP marker development that would enhance the use of SNP-based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer-based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple. C1 [Jeong, YeSol; Lee, SunYoung; Choi, InHye; Chung, SangMin] Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. [Lim, YongPyo] Chungnam Natl Univ, Dept Hort, Taejon 305764, South Korea. [Hur, YoonKang] Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea. [Staub, Jack E.] ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. RP Chung, S (reprint author), Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. EM smchung@dongguk.edu FU Ministry for Food, Agriculture, Forestry, and Fisheries, Republic of Korea [607003-05-4-SB250] FX This work was supported by a grant from the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry, and Fisheries (Grant 607003-05-4-SB250), Republic of Korea. NR 8 TC 1 Z9 2 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2011 VL 130 IS 3 BP 401 EP 403 DI 10.1111/j.1439-0523.2011.01848.x PG 3 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 772HN UT WOS:000291225100016 ER PT J AU Kim, H Ridenour, JB Dunkle, LD Bluhm, BH AF Kim, Hun Ridenour, John B. Dunkle, Larry D. Bluhm, Burton H. TI Regulation of Pathogenesis by Light in Cercospora zeae-maydis: An Updated Perspective SO PLANT PATHOLOGY JOURNAL LA English DT Review DE Cercospora; photoreceptor; stomatal tropism ID GRAY LEAF-SPOT; NEUROSPORA-CRASSA; PLANT-DISEASE; FUNGAL DEVELOPMENT; CIRCADIAN-RHYTHM; WHITE COLLAR-1; MAIZE; TOXIN; CORN; ENVIRONMENT AB The fungal genus Cercospora is one of the most ubiquitous groups of plant pathogenic fungi, and gray leaf spot caused by C. zeae-maydis is one of the most widespread and damaging foliar diseases of maize in the world. While light has been implicated as a critical environmental regulator of pathogenesis in C. zeae-maydis, the relationship between light and the development of disease is not fully understood. Recent discoveries have provided new insights into how light influences pathogenesis and morphogenesis in C. zeae-maydis, particularly at the molecular level. This review is focused on integrating old and new information to provide an updated perspective of how light influences pathogenesis, and provides a working model to explain some of the underlying molecular mechanisms. Ultimately, a thorough molecular-level understanding of how light regulates pathogenesis will augment efforts to manage gray leaf spot by improving host resistance and disease management strategies. C1 [Kim, Hun; Ridenour, John B.; Bluhm, Burton H.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Dunkle, Larry D.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Bluhm, BH (reprint author), Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. EM bbluhm@uark.edu FU USDA-ARS CRIS [3602-22000-015-00D]; NSF [0920287] FX The authors are grateful for financial support from USDA-ARS CRIS project 3602-22000-015-00D (L. Dunkle), and NSF award 0920287 (B. Bluhm). NR 60 TC 2 Z9 2 U1 2 U2 16 PU KOREAN SOC PLANT PATHOLOGY PI SUWON PA NATIONAL INST AGRICULTURAL SCIENCE & TECHNOLOGY, PLANT PATHOLOGY DIVISION, SUWON, 441-707, SOUTH KOREA SN 1598-2254 J9 PLANT PATHOLOGY J JI Plant Pathol. J. PD JUN PY 2011 VL 27 IS 2 BP 103 EP 109 DI 10.5423/PPJ.2011.27.2.103 PG 7 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA 772LJ UT WOS:000291235100001 ER PT J AU Clark, RT MacCurdy, RB Jung, JK Shaff, JE McCouch, SR Aneshansley, DJ Kochian, LV AF Clark, Randy T. MacCurdy, Robert B. Jung, Janelle K. Shaff, Jon E. McCouch, Susan R. Aneshansley, Daniel J. Kochian, Leon V. TI Three-Dimensional Root Phenotyping with a Novel Imaging and Software Platform SO PLANT PHYSIOLOGY LA English DT Article ID SYSTEM ARCHITECTURE; PHOSPHORUS ACQUISITION; RICE; ARABIDOPSIS; CROPS; RECONSTRUCTION; TOMOGRAPHY; EFFICIENCY; TOLERANCE AB A novel imaging and software platform was developed for the high-throughput phenotyping of three-dimensional root traits during seedling development. To demonstrate the platform's capacity, plants of two rice (Oryza sativa) genotypes, Azucena and IR64, were grown in a transparent gellan gum system and imaged daily for 10 d. Rotational image sequences consisting of 40 two-dimensional images were captured using an optically corrected digital imaging system. Three-dimensional root reconstructions were generated and analyzed using a custom-designed software, RootReader3D. Using the automated and interactive capabilities of RootReader3D, five rice root types were classified and 27 phenotypic root traits were measured to characterize these two genotypes. Where possible, measurements from the three-dimensional platform were validated and were highly correlated with conventional two-dimensional measurements. When comparing gellan gum-grown plants with those grown under hydroponic and sand culture, significant differences were detected in morphological root traits (P < 0.05). This highly flexible platform provides the capacity to measure root traits with a high degree of spatial and temporal resolution and will facilitate novel investigations into the development of entire root systems or selected components of root systems. In combination with the extensive genetic resources that are now available, this platform will be a powerful resource to further explore the molecular and genetic determinants of root system architecture. C1 [Clark, Randy T.; Shaff, Jon E.; Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Clark, Randy T.; Aneshansley, Daniel J.] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. [MacCurdy, Robert B.] Cornell Univ, Dept Mech & Aerosp Engn, Ithaca, NY 14853 USA. [Jung, Janelle K.; McCouch, Susan R.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Kochian, LV (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM lvk1@cornell.edu OI Kochian, Leon/0000-0003-3416-089X FU National Science Foundation [DBI-0820624, DBI-0606461, G3008.02] FX This work was supported by the National Science Foundation (Plant Genome grant nos. DBI-0820624 and DBI-0606461 as well as Generation Challenge Program grant no. G3008.02). NR 35 TC 145 Z9 156 U1 12 U2 90 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2011 VL 156 IS 2 BP 455 EP 465 DI 10.1104/pp.110.169102 PG 11 WC Plant Sciences SC Plant Sciences GA 771HC UT WOS:000291146800002 PM 21454799 ER PT J AU Rau, BM Melvin, AM Johnson, DW Goodale, CL Blank, RR Fredriksen, G Miller, WW Murphy, JD Todd, DE Walker, RF AF Rau, Benjamin M. Melvin, April M. Johnson, Dale W. Goodale, Christine L. Blank, Robert R. Fredriksen, Guinevere Miller, Watkins W. Murphy, James D. Todd, Donald E., Jr. Walker, Roger F. TI Revisiting Soil Carbon and Nitrogen Sampling: Quantitative Pits Versus Rotary Cores SO SOIL SCIENCE LA English DT Article DE Soil sampling; soil pit; soil core; coarse fragment; carbon; nitrogen ID FOREST; TOOL AB Increasing atmospheric carbon dioxide and its feedbacks with global climate have sparked renewed interest in quantifying ecosystem carbon (C) budgets, including quantifying belowground pools. Belowground nutrient budgets require accurate estimates of soil mass, coarse fragment content, and nutrient concentrations. It has long been thought that the most accurate measurement of soil mass and coarse fragment content has come from excavating quantitative soil pits. However, this methodology is labor intensive and time consuming. We propose that diamond-tipped rotary cores are an acceptable if not superior alternative to quantitative soil pits for the measurement of soil mass, coarse fragment content, C and total nitrogen (N) concentrations. We tested the rotary core methodology against traditional quantitative pits at research sites in California, Nevada, and New York. We found that soil cores had 16% higher estimates of less than 2-mm soil mass than estimates obtained from quantitative pits. Conversely, soil cores had 8% lower estimates of coarse fragment mass compared with quantitative pits. There were no statistical differences in measured C or N concentrations between the two methods. At the individual site level, differences in estimates for the two methods were more pronounced, but there was no consistent tendency for cores to overestimate or underestimate a soil parameter when compared with quantitative pits. C1 [Rau, Benjamin M.] USDA Forest Serv, La Grande, OR 97850 USA. [Melvin, April M.; Goodale, Christine L.; Fredriksen, Guinevere] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. [Johnson, Dale W.; Miller, Watkins W.; Murphy, James D.; Walker, Roger F.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89512 USA. [Blank, Robert R.] USDA ARS, Reno, NV 89512 USA. [Todd, Donald E., Jr.] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA. RP Rau, BM (reprint author), USDA Forest Serv, La Grande, OR 97850 USA. EM brau02@fs.fed.us FU U.S. Joint Fire Science Program; U.S. Forest Service; Nevada Agricultural Experiment Station; University of Nevada, Reno, Nevada; McIntire-Stennis; Hatch programs FX This work is Contribution No. 47 of the Sagebrush Steppe Treatment Evaluation Project (SageSTEP) and was partially funded by the U.S. Joint Fire Science Program. This research was also supported by the U.S. Forest Service and the Nevada Agricultural Experiment Station, University of Nevada, Reno, Nevada. The New York sampling was supported by funds from the McIntire-Stennis and Hatch programs to Christine L. Goodale. NR 22 TC 13 Z9 13 U1 1 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X J9 SOIL SCI JI Soil Sci. PD JUN PY 2011 VL 176 IS 6 BP 273 EP 279 DI 10.1097/SS.0b013e31821d6d4a PG 7 WC Soil Science SC Agriculture GA 772AR UT WOS:000291202300002 ER PT J AU Zhang, MC He, ZQ Zhao, AQ Zhang, HL Endale, DM Schomberg, HH AF Zhang, Mingchu He, Zhongqi Zhao, Aiqin Zhang, Hailin Endale, Dinku M. Schomberg, Harry H. TI Water-Extractable Soil Organic Carbon and Nitrogen Affected by Tillage and Manure Application SO SOIL SCIENCE LA English DT Article DE Poultry litter; N fertilizer; conventional tillage; no-till; water-extractable organic matter; fluorescence EEM ID FLUORESCENCE SPECTROSCOPY; POULTRY LITTER; CHEMICAL CHARACTERISTICS; CONVENTIONAL-TILLAGE; MATTER FRACTIONS; PARAFAC ANALYSIS; NO-TILLAGE; LAND-USE; EXCITATION; MANAGEMENT AB Water-extractable organic matter (WEOM) contains labile organic carbon (C) and nitrogen (N) and is sensitive to soil management. The objective of this study was to determine the quantity and spectral properties of soil WEOM responding to types of nutrient addition and tillage managements. Soil samples were taken from treatments (conventional tillage, no-tillage, poultry litter, and NH(4)NO(3) application) of a 10-year experiment in Watkinsville, Georgia, at different times and soil depths. Air-dried soil samples were extracted by deionized water followed by filtration (< 0.45 mu m), and soluble C and N, absorptivity at 254 nm, and fluorescence excitation/emission matrix in the extract were determined. Results showed that poultry litter application accumulated higher amounts of water-extractable organic C and N than NH(4)NO(3) application. But no significant difference was found between tillage and no-tillage for both water-extractable C and N. Absorptivity at 254 nm decreased during cropping years for conventional tillage and NH(4)NO(3) application, indicating accumulation of aliphatic organic compounds in WEOM, but the fluorescence-measured humification index showed an opposite trend. For no-tillage and poultry litter application treatments, there was no difference in absorptivity over the sampling time. Parallel factor analysis of excitation/emission matrix data showed that WEOM consisted of three fluorophore components: humic-, fulvic-, and tyrosine-like "compounds,'' and relative distribution of these components differed among treatments and changed over soil depths. The tyrosine-like component tended to accumulate in the lower sampling depth (> 15 cm). Overall, the quantity and spectral properties of WEOM responded to the types of nutrient application and tillage practices. C1 [Zhang, Mingchu; Zhao, Aiqin] Univ Alaska, Dept High Latitude Agr, Sch Nat Resources & Agr Sci, Fairbanks, AK 99775 USA. [He, Zhongqi] USDA ARS, New England Plant Soil & Water Lab, Orono, ME USA. [Zhang, Hailin] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Endale, Dinku M.; Schomberg, Harry H.] USDA ARS J Phil Campbell Sr, Nat Resource Conservat Ctr, Watkinsville, GA USA. RP Zhang, MC (reprint author), Univ Alaska, Dept High Latitude Agr, Sch Nat Resources & Agr Sci, Fairbanks, AK 99775 USA. EM mzhang3@alaska.edu RI Yang, Yang/C-7464-2012; OI He, Zhongqi/0000-0003-3507-5013 NR 31 TC 20 Z9 21 U1 1 U2 29 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X J9 SOIL SCI JI Soil Sci. PD JUN PY 2011 VL 176 IS 6 BP 307 EP 312 DI 10.1097/SS.0b013e31821d6d63 PG 6 WC Soil Science SC Agriculture GA 772AR UT WOS:000291202300006 ER PT J AU He, XH Qi, WY Quinones, B McMahon, S Cooley, M Mandrell, RE AF He, Xiaohua Qi, Wenyuan Quinones, Beatriz McMahon, Stephanie Cooley, Michael Mandrell, Robert E. TI Sensitive Detection of Shiga Toxin 2 and Some of Its Variants in Environmental Samples by a Novel Immuno-PCR Assay SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ESCHERICHIA-COLI O157-H7; HEMOLYTIC-UREMIC SYNDROME; REAL-TIME PCR; EDEMA DISEASE; IDENTIFICATION; CATTLE; GENES; INFECTIONS; OUTBREAK; CULTURE AB Shiga toxin-producing Escherichia coli (STEC) in the environment has been reported frequently. However, robust detection of STEC in environmental samples remains difficult because the numbers of bacteria in samples are often below the detection threshold of the method. We developed a novel and sensitive immuno-PCR (IPCR) assay for the detection of Shiga toxin 2 (Stx2) and Stx2 variants. The assay involves immuno-capture of Stx2 at the B subunit and real-time PCR amplification of a DNA marker linked to a detection antibody recognizing the Stx2 A subunit. The qualitative detection limit of the assay is 0.1 pg/ml in phosphate-buffered saline (PBS), with a quantification range of 10 to 100,000 pg/ml. The IPCR method was 10,000-fold more sensitive than an analogue conventional enzyme-linked immunosorbent assay (ELISA) in PBS. Although the sensitivity of the IPCR for detection of Stx2 was affected by environmental sample matrices of feces, feral swine colons, soil, and water from watersheds, application of the IPCR assay to 23 enriched cultures of fecal, feral swine colon, soil, and watershed samples collected from the environment revealed that the IPCR detected Stx2 in all 15 samples that were shown to be STEC positive by real-time PCR and culture methods, demonstrating a 100% sensitivity and specificity. The modification of the sandwich IPCR we have described in this study will be a sensitive and specific screening method for evaluating the occurrence of STEC in the environment. C1 [He, Xiaohua; Quinones, Beatriz; McMahon, Stephanie; Cooley, Michael; Mandrell, Robert E.] Agr Res Serv, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Qi, Wenyuan] Shanghai Acad Agr Sci, Crop Forest & Fruit Inst, Shanghai, Peoples R China. RP Mandrell, RE (reprint author), Agr Res Serv, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM robert.mandrell@ars.usda.gov FU USDA-ARS [5325-42000-043-00D, 5325-42000-045-00D]; USDA National Research Initiative [2006-55212-16927]; USDA National Institute of Food and Agriculture, Agriculture and Food Research Initiative [2007-35212-18239] FX This research was supported in part by USDA-ARS CRIS projects 5325-42000-043-00D and 5325-42000-045-00D, USDA National Research Initiative competitive grant 2006-55212-16927, and USDA National Institute of Food and Agriculture, Agriculture and Food Research Initiative grant 2007-35212-18239. NR 49 TC 23 Z9 25 U1 0 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2011 VL 77 IS 11 BP 3558 EP 3564 DI 10.1128/AEM.02205-10 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 767HY UT WOS:000290847800002 PM 21441317 ER PT J AU Latorre, AA Van Kessel, JAS Karns, JS Zurakowski, MJ Pradhan, AK Boor, KJ Adolph, E Sukhnanand, S Schukken, YH AF Latorre, Alejandra A. Van Kessel, Jo Ann S. Karns, Jeffrey S. Zurakowski, Michael J. Pradhan, Abani K. Boor, Kathryn J. Adolph, Evin Sukhnanand, Sharinne Schukken, Ynte H. TI Increased In Vitro Adherence and On-Farm Persistence of Predominant and Persistent Listeria monocytogenes Strains in the Milking System SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FIELD GEL-ELECTROPHORESIS; MEXICAN-STYLE CHEESE; BULK TANK MILK; DAIRY FARM; BIOFILM FORMATION; FEBRILE GASTROENTERITIS; INFECTIOUS-DISEASES; PROCESSING PLANTS; BOVINE MASTITIS; WILD BIRDS AB Dairy farms are a reservoir for Listeria monocytogenes, and the reduction of this pathogen at the farm level is important for reducing human exposure. The objectives of this research were to study the diversity of L. monocytogenes strains on a single dairy farm, assess strain dynamics within the farm, identify potential sources of L. monocytogenes in bulk tank milk and milk filters, and assess the adherence abilities of representative strains. A total of 248 L. monocytogenes isolates were analyzed by pulsed-field gel electrophoresis (PFGE). Combined AscI and ApaI restriction analysis yielded 40 PFGE types (strains). The most predominant strains were T (28.6%), D (22.6%), and F (14.9%). A high level of heterogeneity of strains among isolates from fecal (Simpson's index of diversity [SID] = 0.96) and environmental (SID = 0.96) samples was observed. A higher homogeneity of strains was observed among isolates from milk filters (SID = 0.71) and bulk tank milk (SID = 0.65). Six of 17 L. monocytogenes isolates (35.3%) were classified in an in vitro assay as having a "low adherence ability," 9 (52.9%) were classified as having a " medium adherence ability," and 2 (11.8%) were classified as having a " high adherence ability." The L. monocytogenes strains that were predominant and persistent showed significantly better adherence than did strains that were only sporadic, predominant, or persistent (P = 0.0006). Our results suggest that the milking system was exposed to several L. monocytogenes strains from different sources. Only 3 strains, however, were successful in persisting within the milking system, suggesting that some strains are more suitable to that particular ecological environment than others. C1 [Latorre, Alejandra A.; Zurakowski, Michael J.; Pradhan, Abani K.; Adolph, Evin; Sukhnanand, Sharinne; Schukken, Ynte H.] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14850 USA. [Van Kessel, Jo Ann S.; Karns, Jeffrey S.] USDA ARS, Environm Microbial & Food Safety Lab, ANRI, Beltsville, MD 20705 USA. [Boor, Kathryn J.] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Latorre, AA (reprint author), Univ Concepcion, Dept Pathol & Prevent Med, Coll Vet Sci, Av Vincente Mendez 595, Chillan 595, Chile. EM alatorre@udec.cl RI Boor, Kathryn/B-3545-2009; Schukken, Ynte/C-3405-2008 OI Boor, Kathryn/0000-0001-6810-3434; Schukken, Ynte/0000-0002-8250-4194 FU USDA Agricultural Research Service for the Regional Dairy Quality Management Alliance [58-1265-3-155, 58-1265-3-156, 58-1265-3-158, 58-1265-4-020, 58-1265-8-064] FX Financial support for this work was provided by the USDA Agricultural Research Service (agreement no. 58-1265-3-155, 58-1265-3-156, 58-1265-3-158, 58-1265-4-020, and 58-1265-8-064) for the Regional Dairy Quality Management Alliance. NR 46 TC 13 Z9 13 U1 0 U2 11 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2011 VL 77 IS 11 BP 3676 EP 3684 DI 10.1128/AEM.02441-10 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 767HY UT WOS:000290847800016 PM 21441322 ER PT J AU Carter, MQ Brandl, MT Louie, JW Kyle, JL Carychao, DK Cooley, MB Parker, CT Bates, AH Mandrell, RE AF Carter, Michelle Q. Brandl, Maria T. Louie, Jacqueline W. Kyle, Jennifer L. Carychao, Diana K. Cooley, Michael B. Parker, Craig T. Bates, Anne H. Mandrell, Robert E. TI Distinct Acid Resistance and Survival Fitness Displayed by Curli Variants of Enterohemorrhagic Escherichia coli O157:H7 SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CONGO-RED-BINDING; BIOFILM FORMATION; FIBRONECTIN-BINDING; GENE-EXPRESSION; K-12; FIBERS; FIMBRIAE; PROTEIN; O157H7; CELLS AB Curli are adhesive fimbriae of Enterobacteriaceae and are involved in surface attachment, cell aggregation, and biofilm formation. Here, we report that both inter-and intrastrain variations in curli production are widespread in enterohemorrhagic Escherichia coli O157:H7. The relative proportions of curli-producing variants (C(+)) and curli-deficient variants (C(-)) in an E. coli O157: H7 cell population varied depending on the growth conditions. In variants derived from the 2006 U. S. spinach outbreak strains, the shift between the C(+) and C(-) subpopulations occurred mostly in response to starvation and was unidirectional from C(-) to C(+); in variants derived from the 1993 hamburger outbreak strains, the shift occurred primarily in response to oxygen depletion and was bidirectional. Furthermore, curli variants derived from the same strain displayed marked differences in survival fitness: C(+) variants grew to higher concentrations in nutrient-limited conditions than C(-) variants, whereas C(-) variants were significantly more acid resistant than C(+) variants. This difference in acid resistance does not appear to be linked to the curli fimbriae per se, since a csgA deletion mutant in either a C(+) or a C(-) variant exhibited an acid resistance similar to that of its parental strain. Our data suggest that natural curli variants of E. coli O157: H7 carry several distinct physiological properties that are important for their environmental survival. Maintenance of curli variants in an E. coli O157: H7 population may provide a survival strategy in which C(+) variants are selected in a nutrient-limited environment, whereas C(-) variants are selected in an acidic environment, such as the stomach of an animal host, including that of a human. C1 [Carter, Michelle Q.; Brandl, Maria T.; Louie, Jacqueline W.; Kyle, Jennifer L.; Carychao, Diana K.; Cooley, Michael B.; Parker, Craig T.; Bates, Anne H.; Mandrell, Robert E.] Agr Res Serv, Produce Safety & Microbiol Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Carter, MQ (reprint author), Agr Res Serv, Produce Safety & Microbiol Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM michelle.carter@ars.usda.gov FU USDA-ARS [5325-42000-044-00D]; USDA National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239] FX This work was supported in part by USDA-ARS CRIS project 5325-42000-044-00D and by National Research Initiative Competitive Grant numbers 2006-55212-16927 and 2007-35212-18239, from the USDA National Institute of Food and Agriculture. NR 44 TC 32 Z9 32 U1 0 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2011 VL 77 IS 11 BP 3685 EP 3695 DI 10.1128/AEM.02315-10 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 767HY UT WOS:000290847800017 PM 21478320 ER PT J AU Bernacchi, CJ Leakey, ADB Kimball, BA Ort, DR AF Bernacchi, Carl J. Leakey, Andrew D. B. Kimball, Bruce A. Ort, Donald R. TI Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotranspiration and soil water depletion SO ENVIRONMENTAL POLLUTION LA English DT Article DE Tropospheric ozone; Evapotranspiration; Canopy temperature; Soybean; Soil moisture ID AIR CO2 ENRICHMENT; GROUND-LEVEL OZONE; ENERGY-BALANCE; CARBON-DIOXIDE; ELEVATED CO2; STOMATAL CONDUCTANCE; WINTER-WHEAT; CROP YIELDS; PHOTOSYNTHESIS; FIELD AB Tropospheric ozone is increasing in many agricultural regions resulting in decreased stomatal conductance and overall biomass of sensitive crop species. These physiological effects of ozone forecast changes in evapotranspiration and thus in the terrestrial hydrological cycle, particularly in intercontinental interiors. Soybean plots were fumigated with ozone to achieve concentrations above ambient levels over five growing seasons in open-air field conditions. Mean season increases in ozone concentrations ([O(3)]) varied between growing seasons from 22 to 37% above background concentrations. The objective of this experiment was to examine the effects of future [O(3)] on crop ecosystem energy fluxes and water use. Elevated [O(3)] caused decreases in canopy evapotranspiration resulting in decreased water use by as much as 15% in high ozone years and decreased soil water removal. In addition, ozone treatment resulted in increased sensible heat flux in all years indicative of day-time increase in canopy temperature of up to 0.7 degrees C. Published by Elsevier Ltd. C1 [Bernacchi, Carl J.; Ort, Donald R.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Bernacchi, Carl J.; Leakey, Andrew D. B.; Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.; Leakey, Andrew D. B.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Kimball, Bruce A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. RP Bernacchi, CJ (reprint author), USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM bernacch@illinois.edu RI Leakey, Andrew/Q-9889-2016; OI Leakey, Andrew/0000-0001-6251-024X; Bernacchi, Carl/0000-0002-2397-425X NR 50 TC 13 Z9 13 U1 3 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUN PY 2011 VL 159 IS 6 BP 1464 EP 1472 DI 10.1016/j.envpol.2011.03.011 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA 767FE UT WOS:000290839900002 PM 21477906 ER PT J AU Veronesi, M Alberini, A Cooper, JC AF Veronesi, Marcella Alberini, Anna Cooper, Joseph C. TI Implications of Bid Design and Willingness-To-Pay Distribution for Starting Point Bias in Double-Bounded Dichotomous Choice Contingent Valuation Surveys SO ENVIRONMENTAL & RESOURCE ECONOMICS LA English DT Article DE Anchoring; Bid design; Contingent valuation; Double-bounded dichotomous choice format; Monte Carlo simulations; Starting point bias; Willingness-to-pay distribution ID INCENTIVE INCOMPATIBILITY; NONPARAMETRIC-ESTIMATION; QUESTIONS; PREFERENCES; UNCERTAINTY; GOODS AB We examine starting point bias in double-bounded dichotomous choice contingent valuation surveys. We investigate (1) the seriousness of the biases for the location and scale parameters of the willingness-to-pay (WTP) in the presence of starting point bias; (2) whether or not these biases depend on the distribution of WTP and on the bid design; and (3) how well a commonly used diagnostic for starting point bias-a test of the null that bid set dummies entered in the right-hand side of the WTP model are jointly equal to zero-performs under various circumstances. Monte Carlo simulations suggest that the effect of ignoring starting point bias depends on the bid design and on the true distribution of WTP. A well-balanced, symmetric bid design may result in very modest biases even when the anchoring mechanism is very strong. The power of bid set dummies in detecting starting point bias is low. They tend to account for misspecifications in the distribution assumed by the researcher for the latent WTP, rather than capturing the presence of starting point bias. C1 [Veronesi, Marcella] ETH, Inst Environm Decis, CH-8092 Zurich, Switzerland. [Alberini, Anna] Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. [Cooper, Joseph C.] USDA, Econ Res Serv, Washington, DC USA. RP Veronesi, M (reprint author), ETH, Inst Environm Decis, Univ Str 22,CHN K 76-1, CH-8092 Zurich, Switzerland. EM marcella.veronesi@env.ethz.ch NR 36 TC 14 Z9 14 U1 2 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0924-6460 J9 ENVIRON RESOUR ECON JI Environ. Resour. Econ. PD JUN PY 2011 VL 49 IS 2 BP 199 EP 215 DI 10.1007/s10640-010-9430-1 PG 17 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA 762QF UT WOS:000290493800003 ER PT J AU Huang, LH AF Huang, Lihan TI A new mechanistic growth model for simultaneous determination of lag phase duration and exponential growth rate and a new Belehdradek-type model for evaluating the effect of temperature on growth rate SO FOOD MICROBIOLOGY LA English DT Article; Proceedings Paper CT 6th International Conference of Predictive Modeling in Foods (6ICPMF) CY SEP 08-12, 2009 CL Washington, DC DE Growth model; Kinetic analysis; Mathematical modeling ID ESCHERICHIA-COLI O157H7; BEEF; KINETICS; FOOD AB A new mechanistic growth model was developed to describe microbial growth under isothermal conditions. The new mathematical model was derived from the basic observation of bacterial growth that may include lag, exponential, and stationary phases. With this model, the lag phase duration and exponential growth rate of a growth curve were simultaneously determined by nonlinear regression. The new model was validated using Listeria monocytogenes and Escherichia coli O157:H7 in broth or meat. Statistical results suggested that both bias factor (B-f) and accuracy factor (A(f)) of the new model were very close to 1.0. A new Belehdradek-type rate model and the Ratkowsky square-root model were used to describe the temperature dependence of bacterial growth rate. It was observed that the maximum and minimum temperatures were more accurately estimated by a new Belehdradek-type rate model. Further, the inverse of square-roots of lag phases was found proportional to temperature, making it possible to estimate the lag phase duration from the growth temperature. Published by Elsevier Ltd. C1 USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 13 TC 15 Z9 16 U1 1 U2 7 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2011 VL 28 IS 4 SI SI BP 770 EP 776 DI 10.1016/j.fm.2010.05.019 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA 768HI UT WOS:000290924300017 PM 21511137 ER PT J AU Oscar, TP AF Oscar, T. P. TI Plenary lecture: Innovative modeling approaches applicable to risk assessments SO FOOD MICROBIOLOGY LA English DT Article; Proceedings Paper CT 6th International Conference of Predictive Modeling in Foods (6ICPMF) CY SEP 08-12, 2009 CL Washington, DC DE Risk assessment; Rare event modeling; Predictive microbiology; Food safety ID SALMONELLA-TYPHIMURIUM DT104; LOW INITIAL DENSITY; SIMULATION-MODEL; GROUND CHICKEN; GROWTH; MICROFLORA; TEMPERATURE; VALIDATION; SURVIVAL; TIME AB Proper identification of safe and unsafe food at the processing plant is important for maximizing the public health benefit of food by ensuring both its consumption and safety. Risk assessment is a holistic approach to food safety that consists of four steps: 1) hazard identification; 2) exposure assessment; 3) hazard characterization; and 4) risk characterization. Risk assessments are modeled by mapping the risk pathway as a series of unit operations and associated pathogen events and then using probability distributions and a random sampling method to simulate the rare, random, variable and uncertain nature of pathogen events in the risk pathway. To model pathogen events, a rare event modeling approach is used that links a discrete distribution for incidence of the pathogen event with a continuous distribution for extent of the pathogen event. When applied to risk assessment, rare event modeling leads to the conclusion that the most highly contaminated food at the processing plant does not necessarily pose the highest risk to public health because of differences in post-processing risk factors among distribution channels and consumer populations. Predictive microbiology models for individual pathogen events can be integrated with risk assessment models using the rare event modeling method. Published by Elsevier Ltd. C1 Univ Maryland, USDA, ARS, Food Safety Res Unit,Ctr Food Sci & Technol, Princess Anne, MD 21853 USA. RP Oscar, TP (reprint author), Univ Maryland, USDA, ARS, Food Safety Res Unit,Ctr Food Sci & Technol, Room 2111, Princess Anne, MD 21853 USA. EM thomas.oscar@ars.usda.gov NR 14 TC 3 Z9 4 U1 0 U2 2 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2011 VL 28 IS 4 SI SI BP 777 EP 781 DI 10.1016/j.fm.2010.05.017 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA 768HI UT WOS:000290924300018 PM 21511138 ER PT J AU Juneja, VK Marks, H Huang, LH Thippareddi, H AF Juneja, Vijay K. Marks, Harry Huang, Lihan Thippareddi, H. TI Predictive model for growth of Clostridium perfringens during cooling of cooked uncured meat and poultry SO FOOD MICROBIOLOGY LA English DT Article; Proceedings Paper CT 6th International Conference of Predictive Modeling in Foods (6ICPMF) CY SEP 08-12, 2009 CL Washington, DC DE C. perfringens; Predictive modeling; Growth kinetics ID GROUND-BEEF; FOOD AB Comparison of Clostridium perfringens spore germination and outgrowth in cooked uncured products during cooling for different meat species is presented. Cooked, uncured product was inoculated with C. perfringens spores and vacuum packaged. For the isothermal experiments, all samples were incubated in a water bath stabilized at selected temperatures between 10 and 51 degrees C and sampled periodically. For dynamic experiments, the samples were cooled from 54.4 to 27 degrees C and subsequently from 27 to 4 degrees C for different time periods, designated as x and y hours, respectively. The growth models used were based on a model developed by Baranyi and Roberts (1994. A dynamic approach to predicting bacterial growth in food. Int. J. Food Micro. 23, 277-294), which incorporates a constant, referred to as the physiological state constant, q(0). The value of this constant captures the cells' history before the cooling begins. To estimate specific growth rates, data from isothermal experiments were used, from which a secondary model was developed, based on a form of Ratkowsky's 4-parameter equation. The estimated growth kinetics associated with pork and chicken were similar, but growth appeared to be slightly greater in beef; for beef, the maximum specific growth rates estimated from the Ratkowsky curve was about 2.7 log(10) cfu/h, while for the other two species, chicken and pork, the estimate was about 2.2 log(10) cfu/h. Physiological state constants were estimated by minimizing the mean square error of predictions of the log(10) of the relative increase versus the corresponding observed quantities for the dynamic experiments: for beef the estimate was 0.007, while those for pork and chicken the estimates were about 0.014 and 0.011, respectively. For a hypothetical 1.5 h cooling from 54 degrees C to 27 and 5 h to 4 degrees C, corresponding to USDA-FSIS cooling compliance guidelines, the predicted growth (log(10) of the relative increase) for each species was: 1.29 for beef; 1.07 for chicken and 0.95 log(10) for pork. However, it was noticed that for pork in particular, the model using the derived q(0) had a tendency to over-predict relative growth when the observed amount of relative growth was small, and under-predict the relative growth when the observed amount of relative growth was large. To provide more fail-safe estimate, rather than using the derived value of q(0), a value of 0.04 is recommended for pork. Published by Elsevier Ltd. C1 [Juneja, Vijay K.; Huang, Lihan] ARS, Microbial Food Safety Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Marks, Harry] USDA, Food Safety Inspect Serv, Washington, DC 20250 USA. [Thippareddi, H.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. RP Juneja, VK (reprint author), ARS, Microbial Food Safety Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM vjuneja@errc.ars.usda.gov NR 12 TC 15 Z9 15 U1 2 U2 37 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2011 VL 28 IS 4 SI SI BP 791 EP 795 DI 10.1016/j.fm.2010.05.013 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA 768HI UT WOS:000290924300020 PM 21511140 ER PT J AU Velugoti, PR Bohra, LK Juneja, VK Huang, LH Wesseling, AL Subbiah, J Thippareddi, H AF Velugoti, Padmanabha Reddy Bohra, Lalit K. Juneja, Vijay K. Huang, Lihan Wesseling, Audrey L. Subbiah, Jeyamkondan Thippareddi, Harshavardhan TI Dynamic model for predicting growth of Salmonella spp. in ground sterile pork SO FOOD MICROBIOLOGY LA English DT Article; Proceedings Paper CT 6th International Conference of Predictive Modeling in Foods (6ICPMF) CY SEP 08-12, 2009 CL Washington, DC DE Salmonella spp.; Ground pork; Predictive model; Dynamic modeling ID CLOSTRIDIUM-PERFRINGENS; FLUCTUATING TEMPERATURE; LISTERIA-MONOCYTOGENES; BACTERIAL-GROWTH; FOOD AB A predictive model for Salmonella spp. growth in ground pork was developed and validated using kinetic growth data. Salmonella spp. kinetic growth data in ground pork were collected at several isothermal conditions (between 10 and 45 degrees C) and Baranyi model was fitted to describe the growth at each temperature, separately. The maximum growth rates (mu(max)) estimated from the Baranyi model were modeled as a function of temperature using a modified Ratkowsky equation. To estimate bacterial growth under dynamic temperature conditions, the differential form of the Baranyi model, in combination with the modified Ratkowsky equation for rate constants, was solved numerically using fourth order Runge-Kutta method. The dynamic model was validated using five different dynamic temperature profiles (linear cooling, exponential cooling, linear heating, exponential heating, and sinusoidal). Performance measures, root mean squared error, accuracy factor, and bias factor were used to evaluate the model performance, and were observed to be satisfactory. The dynamic model can estimate the growth of Salmonella spp. in pork within a 0.5 log accuracy under both linear and exponential cooling profiles, although the model may overestimate or underestimate at some data points, which were generally < 1 log. Under sinusoidal temperature profiles, the estimates from the dynamic model were also within 0.5 log of the observed values. However, underestimation could occur if the bacteria were exposed to temperatures below the minimum growth temperature of Salmonella spp., since low temperature conditions could alter the cell physiology. To obtain an accurate estimate of Salmonella spp. growth using the models reported in this work, it is suggested that the models be used at temperatures above 7 degrees C, the minimum growth temperature for Salmonella spp. in pork. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Velugoti, Padmanabha Reddy; Bohra, Lalit K.; Wesseling, Audrey L.; Subbiah, Jeyamkondan; Thippareddi, Harshavardhan] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. [Juneja, Vijay K.; Huang, Lihan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Subbiah, Jeyamkondan] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. RP Thippareddi, H (reprint author), Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. EM hthippareddi2@unl.edu NR 16 TC 21 Z9 23 U1 2 U2 14 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2011 VL 28 IS 4 SI SI BP 796 EP 803 DI 10.1016/j.fm.2010.05.007 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA 768HI UT WOS:000290924300021 PM 21511141 ER PT J AU Frye, JG Lindsey, RL Meinersmann, RJ Berrang, ME Jackson, CR Englen, MD Turpin, JB Fedorka-Cray, PJ AF Frye, Jonathan G. Lindsey, Rebecca L. Meinersmann, Richard J. Berrang, Mark E. Jackson, Charlene R. Englen, Mark D. Turpin, Jennifer B. Fedorka-Cray, Paula J. TI Related Antimicrobial Resistance Genes Detected in Different Bacterial Species Co-isolated from Swine Fecal Samples SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID CLASS 1 INTEGRONS; ESCHERICHIA-COLI; SALMONELLA-ENTERICA; ANTIBIOTIC-RESISTANCE; FOOD ANIMALS; CAMPYLOBACTER-JEJUNI; DRUG-RESISTANCE; DNA MICROARRAY; OLIGONUCLEOTIDE MICROARRAY; ENTEROCOCCUS-FAECIUM AB A potential factor leading to the spread of antimicrobial resistance (AR) in bacteria is the horizontal transfer of resistance genes between bacteria in animals or their environment. To investigate this, swine fecal samples were collected on-farm and cultured for Escherichia coli, Salmonella enterica, Campylobacter spp., and Enterococcus spp. which are all commonly found in swine. Forty-nine of the samples from which all four bacteria were recovered were selected yielding a total of 196 isolates for analysis. Isolates were tested for antimicrobial susceptibility followed by hybridization to a DNA microarray designed to detect 775 AR-related genes. E. coli and Salmonella isolated from the same fecal sample had the most AR genes in common among the four bacteria. Genes detected encoded resistance to aminoglycosides (aac(3), aadA1, aadB, and strAB), beta-lactams (ampC, ampR, and bla(TEM)), chloramphenicols (cat and floR), sulfanillic acid (sul1/sulI), tetracyclines (tet(A), tet(D), tet(C), tet(G), and tet(R)), and trimethoprim (dfrA1 and dfh). Campylobacter coli and Enterococcus isolated from the same sample frequently had tet(O) and aphA-3 genes detected in common. Almost half (47%) of E. coli and Salmonella isolated from the same fecal sample shared resistance genes at a significant level (chi(2), p < 0.0000001). These data suggest that there may have been horizontal exchange of AR genes between these bacteria or there may be a common source of AR genes in the swine environment for E. coli and Salmonella. C1 [Frye, Jonathan G.; Lindsey, Rebecca L.; Meinersmann, Richard J.; Berrang, Mark E.; Jackson, Charlene R.; Englen, Mark D.; Turpin, Jennifer B.; Fedorka-Cray, Paula J.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Res Ctr, USDA, Athens, GA 30605 USA. RP Frye, JG (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jonathan.frye@ars.usda.gov RI Frye, Jonathan/I-6382-2013 OI Frye, Jonathan/0000-0002-8500-3395 NR 82 TC 12 Z9 13 U1 0 U2 9 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2011 VL 8 IS 6 BP 663 EP 679 DI 10.1089/fpd.2010.0695 PG 17 WC Food Science & Technology SC Food Science & Technology GA 769MF UT WOS:000291017100002 PM 21385089 ER PT J AU Gorski, L Duhe, JM Flaherty, D AF Gorski, Lisa Duhe, Jessica M. Flaherty, Denise TI The Sigma B Operon Is a Determinant of Fitness for a Listeria monocytogenes Serotype 4b Strain in Soil SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID GENERAL STRESS-RESPONSE; PSEUDOMONAS-FLUORESCENS; ATTACHMENT; COLONIZATION; VIRULENCE; TOLERANCE; TISSUE; RHIZOSPHERE; TEMPERATURE; ADAPTATION AB In nature the foodborne pathogen Listeria monocytogenes lives as a saprophyte where it can contaminate preharvest produce. This environment can present many stresses such as ultraviolet light, variations in temperature and humidity, and oxidative stress from growing plant matter in the soil. The alternative sigma factor Sigma B, encoded by sigB, controls the response to most stresses in L. monocytogenes. Fitness in soil and on radishes sown and grown in contaminated soil was measured in a wild-type and an isogenic sigB operon mutant strain to determine if the sigma factor was necessary for life in these niches. Levels of wild-type and mutant strains were monitored in contaminated soil over the course of radish gestation from seed to mature tuber, and levels on mature radishes were determined. The wild-type strain was able to survive in soil over the 4 weeks of the experiment at levels of 4-7 log CFU/g soil, and the levels of the sigB mutant were reduced by 1-2 log from the wild type. The mutant showed reduced levels in soil by 6 h after inoculation, which was partially recovered when the mutant was complemented, and stayed at a reduced level over the next 4 weeks. Upon harvest, 3-4 log CFU/g of wild-type L. monocytogenes was detected on radish surfaces, and the bacteria could not be washed off under running water. On mature radishes populations of the mutant strain were 1-2 log CFU/g lower than the wild type. The levels on mature radishes reflected the levels in the soil at 4 weeks. The conclusions are that the Sigma B operon is necessary for initial adaptation to the soil environment, and plays a role in maintaining the population, but does not play a role in attachment or colonization of the radish. C1 [Gorski, Lisa; Duhe, Jessica M.; Flaherty, Denise] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. RP Gorski, L (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM lisa.gorski@ars.usda.gov FU U.S. Department of Agriculture (Agricultural Research Service) [5325-42000-044-00D] FX We thank T. Chakraborty, Justus Liebig Universitat Giessen, Giessen, Germany, for the gift of pAUL-A. We thank K. Nguyen and A. Tse for technical assistance. This research was funded by the U.S. Department of Agriculture (Agricultural Research Service CRIS project number 5325-42000-044-00D). NR 39 TC 8 Z9 8 U1 4 U2 12 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2011 VL 8 IS 6 BP 699 EP 704 DI 10.1089/fpd.2010.0752 PG 6 WC Food Science & Technology SC Food Science & Technology GA 769MF UT WOS:000291017100006 PM 21381923 ER PT J AU Quinones, B Swimley, MS Taylor, AW Dawson, ED AF Quinones, Beatriz Swimley, Michelle S. Taylor, Amber W. Dawson, Erica D. TI Identification of Escherichia coli O157 by Using a Novel Colorimetric Detection Method with DNA Microarrays SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID PATHOGEN DETECTION; UNITED-STATES; GENES; PCR; HYBRIDIZATION; INFECTIONS; STRAINS; SAMPLES; CHAIN AB Shiga toxin-producing Escherichia coli O157 is a leading cause of foodborne illness worldwide. To evaluate better methods to rapidly detect and genotype E. coli O157 strains, the present study evaluated the use of ampliPHOX, a novel colorimetric detection method based on photopolymerization, for pathogen identification with DNA microarrays. A low-density DNA oligonucleotide microarray was designed to target stx1 and stx2 genes encoding Shiga toxin production, the eae gene coding for adherence membrane protein, and the per gene encoding the O157-antigen perosamine synthetase. Results from the validation experiments demonstrated that the use of ampliPHOX allowed the accurate genotyping of the tested E. coli strains, and positive hybridization signals were observed for only probes targeting virulence genes present in the reference strains. Quantification showed that the average signal-to-noise ratio values ranged from 47.73 +/- 7.12 to 76.71 +/- 8.33, whereas average signal-to-noise ratio values below 2.5 were determined for probes where no polymer was formed due to lack of specific hybridization. Sensitivity tests demonstrated that the sensitivity threshold for E. coli O157 detection was 1001000 CFU/mL. Thus, the use of DNA microarrays in combination with photopolymerization allowed the rapid and accurate genotyping of E. coli O157 strains. C1 [Quinones, Beatriz; Swimley, Michelle S.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Taylor, Amber W.; Dawson, Erica D.] InDevR Inc, Boulder, CO USA. RP Quinones, B (reprint author), ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St,WAB214L, Albany, CA 94710 USA. EM beatriz.quinones@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service [5325-42000-045]; National Institutes of Health/National Institute of Allergy and Infectious Diseases [U01A1070276] FX This work was supported by the U.S. Department of Agriculture, Agricultural Research Service CRIS project number 5325-42000-045, and by the National Institutes of Health/National Institute of Allergy and Infectious Diseases grant number U01A1070276. The authors would like to thank Edith Pierre-Jerome for excellent technical assistance and to Robert E. Mandrell and Michael Cooley (USDA/ARS/WRRC, Albany, CA) for providing the bacterial strains used in this study. NR 33 TC 10 Z9 10 U1 1 U2 2 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2011 VL 8 IS 6 BP 705 EP 711 DI 10.1089/fpd.2010.0753 PG 7 WC Food Science & Technology SC Food Science & Technology GA 769MF UT WOS:000291017100007 PM 21288130 ER PT J AU Bearson, SMD Bearson, BL Brunelle, BW Sharma, VK Lee, IS AF Bearson, Shawn M. D. Bearson, Bradley L. Brunelle, Brian W. Sharma, Vijay K. Lee, In Soo TI A Mutation in the poxA Gene of Salmonella enterica Serovar Typhimurium Alters Protein Production, Elevates Susceptibility to Environmental Challenges, and Decreases Swine Colonization SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID TRANSFER-RNA SYNTHETASE; ESCHERICHIA-COLI; PHASE VARIATION; SENSOR KINASE; VIRULENCE; NOREPINEPHRINE; ADAPTATION; REGULATORS; MOTILITY; INVASION AB Control of foodborne Salmonella within the farm-retail continuum is a complex issue since over 2500 serovars of Salmonella exist, the host range of Salmonella spp. varies greatly, and Salmonella is environmentally ubiquitous. To identify Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) genes important for pathogen survival, our research group previously screened a signature-tagged mutagenesis bank in an ex vivo swine stomach content assay. A mutation in the poxA gene, a member of the gene family encoding class-II aminoacyl-tRNA synthetases, decreased survival of Salmonella Typhimurium in the ex vivo swine stomach content assay. In the current study, complementation with a plasmid-encoded poxA gene restored survival of the poxA mutant to the level of the parental, wild-type strain. In vivo analysis of the poxA mutant in the natural porcine host revealed significantly reduced fecal shedding of Salmonella, decreased colonization of the tonsils, and decreased detection of the mutant strain in the cecal contents of the pigs at 7 days postinoculation (p<0.05). Body temperature (fever) of the pigs inoculated with wild-type Salmonella Typhimurium was significantly higher than that of pigs inoculated with the poxA mutant (p<0.05). Two-dimensional gel electrophoresis revealed characteristic differences in the protein profile of the poxA mutant relative to the wild-type strain, indicating that deletion of poxA in Salmonella Typhimurium exerts selective effects on translation and/or posttranslational modifications of mRNA species that are necessary for stress survival and colonization of the natural swine host. C1 [Bearson, Shawn M. D.; Brunelle, Brian W.; Sharma, Vijay K.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Bearson, Bradley L.] ARS, Agroecosyst Management Res Unit, Natl Lab Agr & Environm, USDA, Ames, IA 50010 USA. [Lee, In Soo] Hannam Univ, Dept Biotechnol, Taejon, South Korea. RP Bearson, SMD (reprint author), ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave,Room 1403, Ames, IA 50010 USA. EM shawn.bearson@ars.usda.gov FU Agricultural Research Service, U.S. Department of Agriculture FX The authors are grateful to Ann Hoffman, Kellie Winter, Jennifer Jones, and Stephanie Jones for technical assistance. This research was supported by Agricultural Research Service, U.S. Department of Agriculture funds. NR 31 TC 16 Z9 16 U1 0 U2 4 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2011 VL 8 IS 6 BP 725 EP 732 DI 10.1089/fpd.2010.0796 PG 8 WC Food Science & Technology SC Food Science & Technology GA 769MF UT WOS:000291017100010 PM 21348575 ER PT J AU Zhang, BD Alysandratos, KD Angelidou, A Asadi, S Sismanopoulos, N Delivanis, DA Weng, ZY Miniati, A Vasiadi, M Katsarou-Katsari, A Miao, BC Leeman, SE Kalogeromitros, D Theoharides, TC AF Zhang, Bodi Alysandratos, Konstantinos-Dionysios Angelidou, Asimenia Asadi, Shahrzad Sismanopoulos, Nikolaos Delivanis, Danae-Anastasia Weng, Zuyi Miniati, Alexandra Vasiadi, Magdalini Katsarou-Katsari, Alexandra Miao, Benchun Leeman, Susan E. Kalogeromitros, Dimitrios Theoharides, Theoharis C. TI Human mast cell degranulation and preformed TNF secretion require mitochondrial translocation to exocytosis sites: Relevance to atopic dermatitis SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE Atopic dermatitis; degranulation; inflammation; mast cells; mitochondria; substance P; TNF ID FC-EPSILON-RI; SUBSTANCE-P; INFLAMMATORY DISEASE; ACTIVATION; FISSION; SKIN; IGE; STIMULATION; MECHANISMS; INHIBITION AB Background: Mast cells derive from hematopoietic cell precursors and participate in tissue allergic, immune, and inflammatory processes. They secrete many mediators, including preformed TNF, in response to allergic, neuropeptide, and environmental triggers. However, regulation of mast cell degranulation is not well understood. Objective: We investigated the role of mitochondrial dynamics in degranulation of human cultured mast cells. Methods: Human umbilical cord blood-derived mast cells (hCBMCs) and Laboratory of Allergic Diseases 2 (LAD2) mast cells were examined by confocal and differential interference contrast microscopy during activation by IgE/antigen and substance P (SP). Mast cells in control and atopic dermatitis (AD) skin were evaluated by transmission electron microscopy. LAD2 cells were pretreated with mitochondrial division inhibitor, a dynamin-related protein 1 (Drp1) inhibitor, and small interfering RNA for Drp1, which is necessary for mitochondrial fission and translocation. Calcineurin and Drp1 gene expression was analyzed in stimulated LAD2 cells and AD skin biopsies. Results: Stimulation of hCBMCs with IgE/antigen or LAD2 cells with SP leads to rapid (30 minutes) secretion of preformed TNF. Degranulation is accompanied by mitochondrial translocation from a perinuclear location to exocytosis sites. Extracellular calcium depletion prevents these effects, indicating calcium requirement. The calcium-dependent calcineurin and Drp1 are activated 30 minutes after SP stimulation. Reduction of Drp1 activity by mitochondrial division inhibitor and decrease of Drp1 expression using small interfering RNA inhibit mitochondrial translocation, degranulation, and TNF secretion. Mitochondrial translocation is also evident by transmission electron microscopy in skin mast cells from AD biopsies, in which gene expression of calcineurin, Drp1, and SP is higher than in normal skin. Conclusion: Human mast cell degranulation requires mitochondrial dynamics, also implicated in AD. (J Allergy Clin Immunol 2011;127:1522-31.) C1 [Zhang, Bodi; Alysandratos, Konstantinos-Dionysios; Angelidou, Asimenia; Asadi, Shahrzad; Sismanopoulos, Nikolaos; Delivanis, Danae-Anastasia; Weng, Zuyi; Miniati, Alexandra; Vasiadi, Magdalini; Theoharides, Theoharis C.] Tufts Univ, Sch Med, Dept Mol Physiol & Pharmacol, Mol Immunopharmacol & Drug Discovery Lab, Boston, MA 02111 USA. [Zhang, Bodi; Theoharides, Theoharis C.] Tufts Univ, Sch Med, Dept Biochem, Boston, MA 02111 USA. [Miao, Benchun] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Boston, MA 02111 USA. [Theoharides, Theoharis C.] Tufts Univ, Sch Med, Dept Internal Med, Boston, MA 02111 USA. [Theoharides, Theoharis C.] Tufts Med Ctr, Boston, MA USA. [Alysandratos, Konstantinos-Dionysios; Angelidou, Asimenia; Miniati, Alexandra; Vasiadi, Magdalini; Kalogeromitros, Dimitrios; Theoharides, Theoharis C.] Attikon Gen Hosp, Allergy Sect, Allergy Clin Res Unit, Athens, Greece. [Katsarou-Katsari, Alexandra] Univ Athens, Sch Med, A Syggros Hosp, Dept Dermatol 1, GR-10679 Athens, Greece. [Leeman, Susan E.] Boston Univ, Sch Med, Dept Pharmacol, Boston, MA 02215 USA. RP Theoharides, TC (reprint author), Tufts Univ, Sch Med, Dept Mol Physiol & Pharmacol, Mol Immunopharmacol & Drug Discovery Lab, Room M&V 208,136 Harrison Ave, Boston, MA 02111 USA. EM theoharis.theoharides@tufts.edu RI Zhang, Bodi/G-6452-2010; weng, zuyi/J-4147-2015 FU NIH [R01 AR47652]; Galenica SA (Athens, Greece); Hellenic State Scholarships Foundation (Athens, Greece) FX Supported in part by NIH grant R01 AR47652 to T.C.T.; We thank Dr A. S. Kirshenbaum and Dr Dean Metcalfe (National Institutes of Health, Bethesda, Md) for the generous supply of LAD2 mast cells. We also thank Dr Orian Shirihai (Boston University Medical School, Boston, Mass) for kindly providing the Drp1 inhibitor mdivi-1 and for useful discussions and encouragement. Thanks are also due to Dr James A. Marchand (Tufts University, Boston, Mass) for his useful comments on this article. We thank Biovitrum AB (Stockholm, Sweden) for their kind gift of rhSCF. B.Z. is supported by a graduate student fellowship from Galenica SA (Athens, Greece). K.-D. A. and A. A. are recipients of postgraduate scholarships from the Hellenic State Scholarships Foundation (Athens, Greece). Rose Fountotos (Montreal, Canada) participated as a high school trainee during summer 2010. NR 51 TC 53 Z9 54 U1 1 U2 5 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD JUN PY 2011 VL 127 IS 6 BP 1522 EP U318 DI 10.1016/j.jaci.2011.02.005 PG 18 WC Allergy; Immunology SC Allergy; Immunology GA 769WH UT WOS:000291048500032 PM 21453958 ER PT J AU Chavez, PRG Lian, FZ Chung, J Liu, C Paiva, SAR Seitz, HK Wang, XD AF Chavez, Pollyanna R. G. Lian, Fuzhi Chung, Jayong Liu, Chun Paiva, Sergio A. R. Seitz, Helmut K. Wang, Xiang-Dong TI Long-Term Ethanol Consumption Promotes Hepatic Tumorigenesis but Impairs Normal Hepatocyte Proliferation in Rats SO JOURNAL OF NUTRITION LA English DT Article ID RETINOIC ACID CONCENTRATION; ALCOHOLIC LIVER-DISEASE; GROWTH FACTOR-I; PPAR-ALPHA; HEPATOCELLULAR-CARCINOMA; MOLECULAR-MECHANISMS; CELL-PROLIFERATION; C-JUN; EXPRESSION; MICE AB Chronic and excessive alcohol consumption has been related to an increased risk of several cancers, including that of the liver; however, studies in animal models have yet to conclusively determine whether ethanol acts as a tumor promoter in hepatic tumorigenesis. We examined whether prolonged alcohol consumption could act as a hepatic tumor promoter after initiation by diethylnitrosamine (DEN) in a rat model. Male Sprague-Dawley rats were injected with 20 mg DEN/kg body weight 1 wk before introduction of either an ethanol liquid diet or an isoenergic control liquid diet. Hepatic pathological lesions, hepatocyte proliferation, apoptosis, PPAR alpha and PPAR gamma, and plasma insulin-like growth factor 1 IGF-1) levels were assessed after 6 and 10 mo. Mean body and liver weights, plasma IGF-1 concentration, hepatic expressions of proliferating cellular nuclear antigen and Ki-67, and cyclin D1 in ethanol-fed rats were all significantly lower after 10 mo of treatment compared with control rats. In addition, levels of hepatic PPAR gamma protein, not PPAR alpha, were significantly higher in the ethanol-fed rats after prolonged treatment. Although ethanol feeding also resulted in significantly fewer altered hepatic foci, hepatocellular adenoma was detected in ethanol-fed rats at 10 mo, but not in control rats given the same dose of DEN. Together, these results indicate that chronic, excessive ethanol consumption impairs normal hepatocyte proliferation, which is associated with reduced IGF-1 levels, but promotes hepatic carcinogenesis. J. Nutr. 141: 1049-1055, 2011. C1 [Chavez, Pollyanna R. G.; Lian, Fuzhi; Chung, Jayong; Liu, Chun; Paiva, Sergio A. R.; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chung, Jayong] Kyung Hee Univ, Dept Food & Nutr, Seoul 130701, South Korea. [Paiva, Sergio A. R.] Univ Sao Paulo, Dept Med, Botucatu Sch Med, BR-18618000 Botucatu, SP, Brazil. [Seitz, Helmut K.] Univ Heidelberg, Salem Med Ctr, Ctr Alcohol Res Liver Dis & Nutr, D-69120 Heidelberg, Germany. RP Wang, XD (reprint author), Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu RI Lian, Fuzhi/F-9083-2015; Paiva, Sergio/A-5101-2008 OI Lian, Fuzhi/0000-0002-0733-579X; Paiva, Sergio/0000-0003-4412-1990 FU NIH [R01AA12682, R01CA104932]; USDA [1950-51000-064S]; Dietmar-Hopp Foundation; Manfred-Lautenschlaeger Foundation FX Supported by NIH grants R01AA12682 and R01CA104932, USDA grant 1950-51000-064S, and the Dietmar-Hopp and Manfred-Lautenschlaeger Foundations. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of NIH and the USDA. NR 50 TC 9 Z9 9 U1 0 U2 3 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD JUN PY 2011 VL 141 IS 6 BP 1049 EP 1055 DI 10.3945/jn.110.136531 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 768GK UT WOS:000290921700005 PM 21490289 ER PT J AU Farina, EK Kiel, DP Roubenoff, R Schaefer, EJ Cupples, LA Tucker, KL AF Farina, Emily K. Kiel, Douglas P. Roubenoff, Ronenn Schaefer, Ernst J. Cupples, L. Adrienne Tucker, Katherine L. TI Dietary Intakes of Arachidonic Acid and alpha-Linolenic Acid Are Associated with Reduced Risk of Hip Fracture in Older Adults SO JOURNAL OF NUTRITION LA English DT Article ID BONE-MINERAL DENSITY; N-3 FATTY-ACIDS; FOOD FREQUENCY QUESTIONNAIRE; TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; DOCOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; OVARIECTOMIZED RATS; ELDERLY-MEN; FISH-OIL AB PUFA are hypothesized to influence bone health, but longitudinal studies on hip fracture risk are lacking. We examined associations between intakes of PUFA and fish, and hip fracture risk among older adults (n = 904) in the Framingham Osteoporosis Study. Participants (mean age similar to 75 y at baseline) were followed for incident hip fracture from the time they completed the baseline exam (1988-1989) until December 31, 2005. HR and 95% Cl were estimated for energy-adjusted dietary fatty acid exposure variables [(n-3) fatty acids: alpha-linolenic acid (ALA), EPA, DHA, EPA+DHA; (n-6) fatty acids: linoleic acid, arachidonic acid (AA); and the (n-6):(n-3) ratio] and fish intake categories, adjusting for potential confounders and covariates. Protective associations were observed between intakes of ALA (P-trend = 0.02) and hip fracture risk in a combined sample of women and men and between intakes of AA (P-trend = 0.05) and hip fracture risk in men only. Participants in the highest quartile of ALA intake had a 54% lower risk of hip fracture than those in the lowest quartile (Q4 vs. Q1: HR = 0.46; 95% Cl = 0.26-0.83). Men in the highest quartile of AA intake had an 80% lower risk of hip fracture than those in the lowest quartile (04 vs. 01: HR = 0.20; 95% Cl = 0.04-0.96). No significant associations were observed among intakes of EPA, DHA, EPA+DHA, or fish. These findings suggest dietary ALA may reduce hip fracture risk in women and men and dietary AA may reduce hip fracture risk in men. J. Nutr. 141: 1146-1153, 2011. C1 [Tucker, Katherine L.] Northeastern Univ, Bouve Coll Hlth Sci, Boston, MA 02115 USA. [Farina, Emily K.; Roubenoff, Ronenn; Schaefer, Ernst J.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Kiel, Douglas P.] Harvard Univ, Sch Med, Inst Aging Res, Boston, MA 02131 USA. [Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Boston, MA 02118 USA. RP Tucker, KL (reprint author), Northeastern Univ, Bouve Coll Hlth Sci, Boston, MA 02115 USA. EM ki.tucker@neu.edu RI Tucker, Katherine/A-4545-2010; OI Cupples, L. Adrienne/0000-0003-0273-7965 FU National Institute of Arthritis, Musculoskeletal, and Skin Diseases; National Institute on Aging [R01 AR/AG 41398]; (NHLBI/NIH), Bethesda, MD [N01-HC-25195] FX Supported by the National Institute of Arthritis, Musculoskeletal, and Skin Diseases and the National Institute on Aging R01 AR/AG 41398 and the National Heart, Lung, and Blood Institute (NHLBI) Framingham Heart Study (NIH/NHLBI contract N01-HC-25195, Bethesda, MD), Framingham, MA. NR 80 TC 39 Z9 40 U1 0 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2011 VL 141 IS 6 BP 1146 EP 1153 DI 10.3945/jn.110.133728 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 768GK UT WOS:000290921700020 PM 21508210 ER PT J AU Thomas, AP Dunn, TN Oort, PJ Grino, M Adams, SH AF Thomas, Anthony P. Dunn, Tamara N. Oort, Pieter J. Grino, Michel Adams, Sean H. TI Inflammatory Phenotyping Identifies CD11d as a Gene Markedly Induced in White Adipose Tissue in Obese Rodents and Women SO JOURNAL OF NUTRITION LA English DT Article ID DIET-INDUCED OBESITY; ACTIVATED-RECEPTOR-GAMMA; INJURED SPINAL-CORD; INDUCED INSULIN-RESISTANCE; PPAR-GAMMA; INTEGRIN ALPHA(D)BETA(2); MONOCLONAL-ANTIBODY; ADHESION MOLECULES; LEUKOCYTE ADHESION; ADIPOCYTE DEATH AB In severe obesity, white adipose tissue (WAT) inflammation and macrophage infiltration are thought to contribute to WAT and whole-body insulin resistance. Specific players involved in triggering and maintaining inflammation (i.e. those regulating adipokine release and WAT macrophage recruitment, retention, or function) remain to be fully elaborated, and the degree to which moderate obesity promotes WAT inflammation remains to be clarified further. Therefore, we characterized adiposity and metabolic phenotypes in adult male C57BL/6J mice fed differing levels of dietary fat (10, 45, and 60% of energy) for 12 wk, concurrent with determinations of WAT inflammation markers and mRNA expression of leukocyte-derived integrins (CD11b, CD11c, CD11d) involved in macrophage extravasation and tissue macrophage homing/retention. As expected, a lard-based, very high-fat diet (60% energy) significantly increased adiposity and glucose intolerance compared with 10% fat-fed controls, coincident with higher retroperitoneal (RP) WAT transcript levels for proinflammatory factors and macrophage markers, including TNF alpha and CD68 mRNA, which were similar to 3- and similar to 15-fold of control levels, respectively (P < 0.001). Mice fed the 45% fat diet had more moderate obesity, less glucose intolerance, and lower WAT macrophage/inflammatory marker m RNA abundances compared with 60% fat-fed mice; TNF alpha and CD68 mRNA levels were similar to 2- and similar to 5-fold of control levels (P < 0.01). Relative WAT expression of CD11d was massively induced by obesity to an extent greater than any other inflammatory marker (to >300-fold of controls in the 45 and 60% fat groups) (P < 0.0001) and this induction was WAT specific. Because we found that CD11d expression also increased in RP-WAT of Zucker obese rats and in the subcutaneous WAT of obese adult women, this appears to be a common feature of obesity. Observed correlations of WAT macrophage transcript marker abundances with body weight in lean to modestly obese mice raises an interesting possibility that the activities of at least some WAT macrophages are closely linked to the normal adipose remodeling that is a requisite for changes in WAT energy storage capacity. J. Nutr. 141: 1172-1180, 2011. C1 [Thomas, Anthony P.; Dunn, Tamara N.; Oort, Pieter J.; Adams, Sean H.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Thomas, Anthony P.; Dunn, Tamara N.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Grino, Michel] INSERM, U626, Marseille, France. [Grino, Michel] Univ Aix Marseille 2, Fac Med, F-13385 Marseille, France. RP Adams, SH (reprint author), USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. EM sean.h.adams@ars.usda.gov FU USDA-ARS [5306-51530-019-00]; National Dairy Council FX Supported in part by the intramural USDA-ARS Project 5306-51530-019-00 and the National Dairy Council (grant administered by the Dairy Research Institute). USDA is an equal opportunity provider and employer. NR 67 TC 15 Z9 15 U1 1 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2011 VL 141 IS 6 BP 1172 EP 1180 DI 10.3945/jn.110.127068 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 768GK UT WOS:000290921700024 PM 21508205 ER PT J AU Shogren, RL Selling, G Willett, JL AF Shogren, Randal L. Selling, Gordon Willett, J. L. TI Effect of Orientation on the Morphology and Mechanical Properties of PLA/Starch Composite Filaments SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Starch; Polylactic acid; Fibers; Extrusion ID POLY(LACTIC ACID); RENEWABLE RESOURCES; PHYSICAL-PROPERTIES; REACTIVE EXTRUSION; STARCH COMPOSITES; BLENDS; ANHYDRIDE; POLYMERS; PLASTICS; FILMS AB Polylactic acid (PLA)/starch fibers were produced by twin screw extrusion of PLA with granular or gelatinized starch/glycerol followed by drawing through a set of winders with an intermediate oven. At 30% starch, fibers drawn 2-5x were highly flexible (elongation 20-100%) while undrawn filaments were brittle (elongation 2-9%). Tensile strength and moduli increased with increasing draw ratio but decreased with increasing starch content. Mechanical properties were better for composites made with gelatinized starch/glycerol than granular starch. In conclusion, orientation greatly increases the flexibility of PLA/starch composites and this may be useful not only in fibers but also possibly in molded articles. Other advantages of starch addition could include fiber softness without added plasticizer, moisture/odor absorbency and as a carrier for active compounds. C1 [Shogren, Randal L.; Selling, Gordon] USDA ARS NCAUR, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Willett, J. L.] USDA ARS, Off Director, Midwest Area, Peoria, IL 61604 USA. RP Shogren, RL (reprint author), USDA ARS NCAUR, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Randy.Shogren@ars.usda.gov NR 32 TC 2 Z9 2 U1 4 U2 20 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 J9 J POLYM ENVIRON JI J. Polym. Environ. PD JUN PY 2011 VL 19 IS 2 BP 329 EP 334 DI 10.1007/s10924-010-0267-z PG 6 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA 769UY UT WOS:000291045000001 ER PT J AU Basta, AH El-Saied, H Winandy, JE Sabo, R AF Basta, Altaf H. El-Saied, Houssni Winandy, Jerrold E. Sabo, Ronald TI Preformed Amide-containing biopolymer for Improving the Environmental Performance of Synthesized Urea-formaldehyde in Agro-fiber Composites SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Modified-HCHO-based adhesive; Urea-formaldehyde; Biopolymers; Agro-based composite; Environment; Free formaldehyde ID LIGNOCELLULOSIC COMPOSITES; ADHESIVES; RESINS AB Investigations have continued for production high performance agro-based composites using environmentally acceptable approaches. This study examines the role of adding amide-containing biopolymers during synthesis of urea-formaldehyde (UF) on properties of adhesive produced, especially its adhesion potential. The environmental performance of UF-resin synthesized in the presence of modified amide-containing biopolymer was evaluated by evaluating the free-HCHO of both adhesive (during processing) and of the eventual engineered composite product. Also, the benefits of this synthesis-modified adhesive in enhancing the bondability of sugar-cane fibers used in engineered composite panels was evaluated and compared to using UF-resin. The results obtained show that, static bending of the produced composites varied from 27.7 to 33.13 N/mm(2) of modulus of rupture (MOR) and from 2860 to 3374 N/mm(2) of Modulus of Elasticity (MOE); while for internal bond (IB) it's varied from 0.64 to 0.866 N/mm(2). Based on the ANSI and EN Standards modified UF-based agro composites produced meet the performance requirements for high grade particleboards with respect to static bending strength. These agro-based composite also tested out as having free-HCHO values of similar to 13 mg/100 g board. C1 [Basta, Altaf H.; El-Saied, Houssni] Natl Res Ctr, Cellulose & Paper Dept, Cairo 12622, Egypt. [Winandy, Jerrold E.] Univ Minnesota, St Paul, MN 55108 USA. [Sabo, Ronald] US Forest Serv, Madison, WI USA. RP Basta, AH (reprint author), Natl Res Ctr, Cellulose & Paper Dept, Cairo 12622, Egypt. EM altaf_basta@yahoo.com OI Basta, Altaf/0000-0002-4030-8486; Basta, Altaf/0000-0003-1876-4378; El saied, Houssni/0000-0002-1833-5795 FU Egypt-U.S. Science and Technology Program; Egyptian Ministry of Scientific Research; U.S. Department of State [280] FX This research work was carried out under grant from Egypt-U.S. Science and Technology Program and sponsored by the Egyptian Ministry of Scientific Research and the U.S. Department of State with Contact/Agreement No. 280. NR 23 TC 16 Z9 18 U1 0 U2 10 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 J9 J POLYM ENVIRON JI J. Polym. Environ. PD JUN PY 2011 VL 19 IS 2 BP 405 EP 412 DI 10.1007/s10924-011-0286-4 PG 8 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA 769UY UT WOS:000291045000009 ER PT J AU Sekhar, RV Patel, SG D'Amico, S Shi, JJ Balasubramanyam, A Rehman, K Jahoor, F Visnegarwala, F AF Sekhar, Rajagopal V. Patel, Sanjeet G. D'Amico, Susana Shi, Jianjian Balasubramanyam, Ashok Rehman, Khaleel Jahoor, Farook Visnegarwala, Fehmida TI Effects of rosiglitazone on abnormal lipid kinetics in HIV-associated dyslipidemic lipodystrophy: a stable isotope study SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID ACTIVE ANTIRETROVIRAL THERAPY; HUMAN-IMMUNODEFICIENCY-VIRUS; PLACEBO-CONTROLLED TRIAL; ADIPOSE-TISSUE; MYOCARDIAL-INFARCTION; INSULIN SENSITIVITY; FAT DISTRIBUTION; INFECTED MEN; DOUBLE-BLIND; GLUCOCORTICOIDS AB HIV-associated dyslipemic lipodystrophy (HADL) is a heterogeneous syndrome of fat redistribution, hypertriglyceridemia, and insulin resistance, associated with markedly accelerated rates of lipolysis, intraadipocyte and intrahepatic reesterification, and very low-density lipoprotein triglyceride synthesis and release. The objective of the study was to determine if rosiglitazone can ameliorate these lipid kinetic defects in patients with HADL. infusions of [C-13(1)]palmitate and [H-2(5)]glycerol were used to measure total and net lipolysis, adipocyte and hepatic reesterification, and plasma free fatty acid (FFA) oxidation in 9 men with HADL, before and after 3 months of treatment with rosiglitazone (8 mg/d). Rosiglitazone treatment significantly increased both total lipolysis (R-a FFA(total) from 3.37 +/- 0.40 to 4.57 +/- 0.68 mmol FFA per kilogram fat per hour, P < .05) and adipocyte reesterification (1.25 +/- 0.35 to 2.43 +/- 0.65 mmol FFA per kilogram fat per hour, P < .05). However, there was no change in net lipolysis (R-a FFA(net) 2.47 +/- 0.43 to 2.42 +/- 0.37 mmol FFA per kilogram fat per hour), plasma FFA oxidation (0.30 +/- 0.046 to 0.32 +/- 0.04 mmol FFA per kilogram lean body mass per hour), or FFA flux available for hepatic reesterification (0.59 +/- 0.07 to 0.56 +/- 0.10 mmol FFA per kilogram fat per hour). There were significant decreases in fasting plasma insulin concentrations and insulin resistance, but not in fasting plasma lipid or glucose concentrations. There was a significant decrease in waist to hip ratio (0.98 +/- 0.02 to 0.95 +/- 0.02, P < .05) consistent with a significant increase in hip circumference (0.93 +/- 0.02 to 0.95 +/- 0.02 m, P < .05), without change in waist circumference. Rosiglitazone significantly increased adipocyte reesterification and improved insulin sensitivity, but the potential benefit of these changes was compromised by increase in total lipolysis. Combining rosiglitazone with agents designed to blunt lipolysis could expand depleted peripheral adipose depots in patients with HIV lipodystrophy. (C) 2011 Elsevier Inc. All rights reserved. C1 [Sekhar, Rajagopal V.] Baylor Coll Med, Div Endocrinol Diabet & Metab, Translat Metab Unit, Diabet & Endocrinol Res Ctr, Houston, TX 77030 USA. [Sekhar, Rajagopal V.; D'Amico, Susana; Balasubramanyam, Ashok] Ben Taub Gen Hosp, Endocrine Serv, Houston, TX 77030 USA. [Jahoor, Farook] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Jahoor, Farook] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Visnegarwala, Fehmida] Baylor Coll Med, Dept Med, Infect Dis Sect, Houston, TX 77030 USA. RP Sekhar, RV (reprint author), Baylor Coll Med, Div Endocrinol Diabet & Metab, Translat Metab Unit, Diabet & Endocrinol Res Ctr, ABBR R-604, Houston, TX 77030 USA. EM rsekhar@bcm.edu FU Glaxo Smithkline; Baylor College of Medicine; National Institutes of Health [M01-RR00188]; General Clinical Research Center; NIH-Diabetes and Endocrinology Research Center at Baylor College of Medicine [P30DK079638] FX We thank Dina Harleaux, Lynne Scott, Varsha Patel, and the nursing staff of the Baylor General Clinical Research Center for excellent care of subjects and meticulous attention to protocol. This work was supported by an investigator-initiated research grant from Glaxo Smithkline (to FV) and Baylor College of Medicine Seed Grant (to RVS). This work also received support from the National Institutes of Health, M01-RR00188, General Clinical Research Center, and was supported in part by the NIH-Diabetes and Endocrinology Research Center (P30DK079638), at Baylor College of Medicine. NR 36 TC 6 Z9 6 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD JUN PY 2011 VL 60 IS 6 BP 754 EP 760 DI 10.1016/j.metabol.2010.07.023 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 770GX UT WOS:000291076100003 PM 20832829 ER PT J AU Lindsey, RL Frye, JG Thitaram, SN Meinersmann, RJ Fedorka-Cray, PJ Englen, MD AF Lindsey, Rebecca L. Frye, Jonathan G. Thitaram, Sutawee N. Meinersmann, Richard J. Fedorka-Cray, Paula J. Englen, Mark D. TI Characterization of Multidrug-Resistant Escherichia coli by Antimicrobial Resistance Profiles, Plasmid Replicon Typing, and Pulsed-Field Gel Electrophoresis SO MICROBIAL DRUG RESISTANCE LA English DT Article ID SPECTRUM BETA-LACTAMASES; ANIMAL ORIGIN; SULFONAMIDE RESISTANCE; HUMAN HEALTH; SALMONELLA; BACTERIA; ENTEROBACTERIACEAE; IDENTIFICATION; EPIDEMIOLOGY; GENES AB The objective of this study was to examine the distribution of multidrug resistance in Escherichia coli in relation to plasmid replicon types, animal sources, and genotypes. E. coli isolates (n = 35) from seven different animal sources were selected and tested for susceptibility to 15 antimicrobials; pulsed-field gel electrophoresis was used to determine genetic relationships among the E. coli isolates. Plasmid types based on their incompatibility (Inc) replicon types were determined, and linkage disequilibrium analysis was performed for antimicrobial resistance profiles, replicon types, and animal source. A high degree of genotypic diversity was observed: 34 different pulsed-field gel electrophoresis types among the 35 isolates examined. Twelve different plasmid Inc types were detected, and all isolates carried at least one replicon type. IncF (n = 25; 71.4%) and IncFIB (n = 19; 54.3%) were the most common replicon types identified. Chloramphenicol resistance was significantly linked with four Inc types (A/C, FIIA, F, and Y), and amoxicillin/clavulanic acid was linked with three Inc types (B/O, P and Y). Resistance to any other antimicrobial was linked to two or fewer replicon types. The isolate source was linked with resistance to seven antimicrobials and IncI1. We conclude that commensal E. coli from animal sources are highly variable genotypically and are reservoirs of a diverse array of plasmids carrying antimicrobial resistance. C1 [Lindsey, Rebecca L.; Frye, Jonathan G.; Thitaram, Sutawee N.; Meinersmann, Richard J.; Fedorka-Cray, Paula J.; Englen, Mark D.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. RP Englen, MD (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM mark.englen@ars.usda.gov RI Frye, Jonathan/I-6382-2013 OI Frye, Jonathan/0000-0002-8500-3395 NR 31 TC 12 Z9 12 U1 0 U2 6 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD JUN PY 2011 VL 17 IS 2 BP 157 EP 163 DI 10.1089/mdr.2010.0148 PG 7 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA 769GT UT WOS:000291001100004 PM 21332363 ER PT J AU Lu, SW Friesen, TL Faris, JD AF Lu, Shunwen Friesen, Timothy L. Faris, Justin D. TI Molecular characterization and genomic mapping of the pathogenesis-related protein 1 (PR-1) gene family in hexaploid wheat (Triticum aestivum L.) SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE PR proteins; Plant defense; Host-pathogen interactions; Common wheat; Physical mapping; Differential expression ID EXPRESSED SEQUENCE TAGS; ACQUIRED-RESISTANCE; POLYPLOID WHEAT; SIGNAL PEPTIDE; CELL-DEATH; ZEA-MAYS; ARABIDOPSIS; TOBACCO; TOMATO; IDENTIFICATION AB The group 1 pathogenesis-related (PR-1) proteins, known as hallmarks of defense pathways, are encoded by multigene families in plants as evidenced by the presence of 22 and 32 PR-1 genes in the finished Arabidopsis and rice genomes, respectively. Here, we report the initial characterization and mapping of 23 PR-1-like (TaPr-1) genes in hexaploid wheat (Triticum aestivum L.), which possesses one of the largest (> 16,000 megabases) genomes among monocot crop plants. Sequence analysis revealed that the 23 TaPr-1 genes all contain intron-free open reading frames that encode a signal peptide at the N-terminus and a conserved PR-1-like domain. Phylogenetic analysis indicated that TaPr-1 genes form three major monophyletic groups along with their counterparts in other monocots; each group consists of genes encoding basic, basic with a C-terminal extension, and acidic PR-1 proteins, respectively, suggesting diversity and conservation of PR-1 gene functions in monocot plants. Mapping analysis assisted by untranslated region-specified discrimination (USD) markers and various cytogenetic stocks located the 23 TaPr-1 genes to seven different chromosomes, with the majority mapping to chromosomes of homoeologous groups 5 and 7. Reverse transcriptase (RT)-PCR analysis revealed that 12 TaPr-1 genes were induced or up-regulated upon pathogen challenge. Together, this study provides insights to the origin, evolution, homoeologous relationships, and expression patterns of the TaPr-1 genes. The data presented provide critical information for further genome-wide characterization of the wheat PR-1 gene family and the USD markers developed will facilitate genetic and functional analysis of PR-1 genes associated with plant defense and/or other important traits. C1 [Lu, Shunwen; Friesen, Timothy L.; Faris, Justin D.] USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. RP Lu, SW (reprint author), USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. EM Shunwen.Lu@ars.usda.gov FU USDA-Agricultural Research Service CRIS project [5442-22000-033-00D] FX We thank Jamie Hauff, Kelsey Dunnell and Rachel Lindgren for technical assistance, and Michael Edwards for reviewing the manuscript. This research was supported by the USDA-Agricultural Research Service CRIS project 5442-22000-033-00D. NR 74 TC 17 Z9 18 U1 0 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD JUN PY 2011 VL 285 IS 6 BP 485 EP 503 DI 10.1007/s00438-011-0618-z PG 19 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 769TD UT WOS:000291040300005 PM 21516334 ER PT J AU Johnson, ET Dowd, PF Liu, ZL Musser, RO AF Johnson, Eric T. Dowd, Patrick F. Liu, Z. Lewis Musser, Richard O. TI Comparative transcription profiling analyses of maize reveals candidate defensive genes for seedling resistance against corn earworm SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Defense; Insect; Maize; Seedling; Microarray ID INSECT RESISTANCE; SCHIZAPHIS-GRAMINUM; HYDROXAMIC ACIDS; DIMBOA CONTENT; PLANT; PROTEIN; LEAF; ARABIDOPSIS; INHIBITORS; BORER AB As maize seedlings germinate into the soil, they encounter an environment teeming with insects seeking rich sources of nutrition. Maize presumably has developed a number of molecular mechanisms to ensure survival at the beginning of its life cycle. Comparative transcription analysis using microarrays was utilized to document the expression of a number of genes with potential defensive functions in seedling tissue. In addition to elevated levels of the genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), an anti-insect resistance molecule, other highly expressed genes in the seedling encode the following putative defensive proteins: defensin, hydroxyproline and proline-rich protein, thaumatin-like protein, lipase, cystatin, protease inhibitor, and a variety of proteases. The potential resistance genes identified occurred mainly on chromosomes 1 and 5 in the B73 genome. Analysis of promoters of seven DIMBOA biosynthetic genes identified three transcription factor binding sites that are possibly involved in regulation of the DIMBOA biosynthetic pathway. The results indicate that maize employs a wide variety of potential resistance mechanisms in seedling tissue to resist a possible insect attack. C1 [Johnson, Eric T.; Dowd, Patrick F.] ARS, Crop Bioprotect Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Liu, Z. Lewis] ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Musser, Richard O.] Western Illinois Univ, Dept Biol Sci, Macomb, IL 61455 USA. RP Johnson, ET (reprint author), ARS, Crop Bioprotect Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM eric.t.johnson@ars.usda.gov OI Johnson, Eric/0000-0002-1665-6229; Musser, Richard/0000-0002-9331-4249 FU National Science Foundation Plant Genome Research Initiative [0820367] FX We thank David Lee, Mark Doehring, and Dr. Menggen Ma for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. Funding was provided by the National Science Foundation Plant Genome Research Initiative (No. 0820367 to ROM) for statistical software. NR 52 TC 8 Z9 8 U1 1 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD JUN PY 2011 VL 285 IS 6 BP 517 EP 525 DI 10.1007/s00438-011-0626-z PG 9 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 769TD UT WOS:000291040300007 PM 21556895 ER PT J AU Johnson, KN Kleinman, PJA Beegle, DB Elliott, HA Saporito, LS AF Johnson, Keisha N. Kleinman, Peter J. A. Beegle, Douglas B. Elliott, Herschel A. Saporito, Lou S. TI Effect of dairy manure slurry application in a no-till system on phosphorus runoff SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Phosphorus; Manure application; Surface runoff; No-till ID WATER-EXTRACTABLE PHOSPHORUS; LIQUID SWINE MANURE; SURFACE; RAINFALL; LOSSES; BROADCAST; MANAGEMENT; FERTILIZER; YIELD; SOILS AB Incorporation of manure slurry under reduced tillage conditions remains a challenge in the northeastern US. New technologies to directly incorporate slurry are available but their agronomic and environmental benefits have generally not been quantified. This study evaluated the effects of five manure slurry application methods on phosphorus (P) loss in runoff (broadcasting with and without incorporation by tillage, shallow disk injection, banded application and aeration, and pressurized injection) and a control (no manure). Research was conducted over a 2 year period in central Pennsylvania on a well-drained Hagerstown silt loam (fine, mixed, semiactive, mesic Typic Hapludalf) under corn (Zea mays L.) production. Approximately 72 h after dairy (Bos Taurus) slurry application (56,000 l ha(-1)) to 10 x 13 m plots, a single rainfall simulation (68 mm h(-1)) was conducted in triplicate on 10 x 2 m areas within the plots. Trends in total P losses in runoff (kg ha(-1)) from plots varied between years and treatments. Aeration yielded lower losses than all other treatments in 2006 and was amongst the lowest in 2007 with losses statistically similar to shallow disk and pressure injection. Remarkably, few differences were apparent in losses of dissolved reactive P between treatments, reflecting high variability in runoff depths. Indeed, variability in runoff depths resulted in some unexpected trends, including high loads from the unamended control and modest loads from the tillage treatment. Results highlight tradeoffs in alternative manure slurry application practices but point to the potential to significantly lower runoff P losses from reduced tillage systems receiving manure slurry. C1 [Johnson, Keisha N.; Beegle, Douglas B.] Penn State Univ, Dept Crop & Soil Sci, University Pk, PA 16802 USA. [Kleinman, Peter J. A.; Saporito, Lou S.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. [Elliott, Herschel A.] Penn State Univ, Dept Agr & Biosyst Engn, University Pk, PA 16802 USA. RP Johnson, KN (reprint author), Penn State Univ, Dept Crop & Soil Sci, Agr Sci & Ind Bldg, University Pk, PA 16802 USA. EM knj107@psu.edu NR 39 TC 11 Z9 11 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD JUN PY 2011 VL 90 IS 2 BP 201 EP 212 DI 10.1007/s10705-011-9422-8 PG 12 WC Soil Science SC Agriculture GA 768NV UT WOS:000290942700003 ER PT J AU Spargo, JT Cavigelli, MA Mirsky, SB Maul, JE Meisinger, JJ AF Spargo, John T. Cavigelli, Michel A. Mirsky, Steven B. Maul, Jude E. Meisinger, John J. TI Mineralizable soil nitrogen and labile soil organic matter in diverse long-term cropping systems SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Chemically-labile organic matter (CLOM); Organic farming; Particulate organic matter (POM); Soil mineralizable nitrogen ID CONVENTIONAL FARMING SYSTEMS; DOUBLE EXPONENTIAL MODEL; MID-ATLANTIC REGION; NO-TILL MANAGEMENT; CARBON SEQUESTRATION; USE EFFICIENCY; COASTAL-PLAIN; FIELD CROPS; AVAILABILITY; DYNAMICS AB Sustainable soil fertility management depends on long-term integrated strategies that build and maintain soil organic matter and mineralizable soil N levels. These strategies increase the portion of crop N needs met by soil N and reduce dependence on external N inputs required for crop production. To better understand the impact of management on soil N dynamics, we conducted field and laboratory research on five diverse management systems at a long-term study in Maryland, the USDA- Agricultural Research Service Beltsville Farming Systems Project (FSP). The FSP is comprised of a conventional no-till corn (Zea mays L.)-soybean (Glycine max L.)-wheat (Triticum aestivum L.)/double-crop soybean rotation (NT), a conventional chisel-till corn-soybean-wheat/soybean rotation (CT), a 2 year organic corn-soybean rotation (Org2), a 3 year organic corn-soybean-wheat rotation (Org3), and a 6 year organic corn-soybean-wheat-alfalfa (Medicago sativa L.) (3 years) rotation (Org6). We found that total potentially mineralizable N in organic systems (average 315 kg N ha(-1)) was significantly greater than the conventional systems (average 235 kg N ha(-1)). Particulate organic matter (POM)-C and -N also tended to be greater in organic than conventional cropping systems. Average corn yield and N uptake from unamended (minus N) field microplots were 40 and 48%, respectively, greater in organic than conventional grain cropping systems. Among the three organic systems, all measures of N availability tended to increase with increasing frequency of manure application and crop rotation length (Org2 < Org3 a parts per thousand currency sign Org6) while most measures were similar between NT and CT. Our results demonstrate that organic soil fertility management increases soil N availability by increasing labile soil organic matter. Relatively high levels of mineralizable soil N must be considered when developing soil fertility management plans for organic systems. C1 [Spargo, John T.; Cavigelli, Michel A.; Mirsky, Steven B.; Maul, Jude E.] ARS, Sustainable Agr Syst Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Meisinger, John J.] ARS, Environm Management & By Prod Utilizat Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. RP Spargo, JT (reprint author), ARS, Sustainable Agr Syst Lab, Anim & Nat Resources Inst, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM john.spargo@ars.usda.gov NR 72 TC 29 Z9 30 U1 5 U2 55 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD JUN PY 2011 VL 90 IS 2 BP 253 EP 266 DI 10.1007/s10705-011-9426-4 PG 14 WC Soil Science SC Agriculture GA 768NV UT WOS:000290942700007 ER PT J AU Yang, YZ Singer, SD Liu, ZR AF Yang, Yazhou Singer, Stacy D. Liu, Zongrang TI Evaluation and comparison of the insulation efficiency of three enhancer-blocking insulators in plants SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Enhancer-blocking insulators; Enhancer-promoter interaction; Insulation efficiency; Plant transformation; Arabidopsis ID TISSUE-SPECIFIC EXPRESSION; DROSOPHILA-MELANOGASTER; TRANSGENE EXPRESSION; GENE-EXPRESSION; ADENOVIRUS VECTORS; PROMOTER SEQUENCES; BITHORAX COMPLEX; HUMAN GENOME; ARABIDOPSIS; ELEMENT AB Enhancer-promoter interactions have the potential to compromise the precise engineering of gene function and agronomically important traits in crops; therefore, effective enhancer-blocking insulators are required in transgene constructs to block such interference. In this study, we evaluated and compared the insulation efficiency of three enhancer-blocking insulators using an enhancer activation assay in which a flower-specific AGAMOUS second intron-derived promoter (AGIP) that drives a beta-glucuronidase (GUS) reporter gene is activated in vegetative tissues by an adjacent CaMV35S enhancer. Of the insulators analyzed, both the EXOB fragment from the lambda genome and the TBS from petunia were found to effectively block enhancer activation, while the 16 bp NI29 fragment from Arabidopsis inserted either as a single copy or tandem repeat in the same assay vector failed to do the same as revealed by the detection of strong GUS expression in the leaves. PCR amplification and DNA sequencing analyses demonstrated that there were no deletions or mutation events within the transgenic NI29 sequence, indicating that the failure of NI29 to block 35S enhancer activation was not the result of gene rearrangements or sequence mutations. Quantitative analysis of GUS reporter expression at both the enzymatic and transcriptional levels exemplified that the EXOB is on average four times more efficient than the TBS in blocking enhancer-promoter communication. This highly effective insulator has an immediate application for remedying enhancer-promoter interactions within transformation vectors composed of multiple transcription units. C1 [Yang, Yazhou; Singer, Stacy D.; Liu, Zongrang] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Yang, Yazhou] NW A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. [Singer, Stacy D.] Cornell Univ, New York State Agr Expt Stn, Dept Plant Pathol, Geneva, NY 14456 USA. RP Liu, ZR (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM zongrang.liu@ars.usda.gov FU United States Department of Agriculture (USDA)-Agricultural Research Service Headquarter; USDA National Institute of Food and Agriculture [2006-03701] FX We would like to thank Mr. Dennis Bennett for his excellent technical assistance. This study was funded by the United States Department of Agriculture (USDA)-Agricultural Research Service Headquarter 2007 classes of postdoctoral grants and the Biotechnology Risk Assessment Program Grant (2006-03701) from the USDA National Institute of Food and Agriculture. NR 44 TC 6 Z9 6 U1 0 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD JUN PY 2011 VL 105 IS 3 BP 405 EP 414 DI 10.1007/s11240-010-9880-8 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA 768NT UT WOS:000290942500014 ER PT J AU Crouch, JA Szabo, LJ AF Crouch, Jo Anne Szabo, Les J. TI Real-Time PCR Detection and Discrimination of the Southern and Common Corn Rust Pathogens Puccinia polysora and Puccinia sorghi SO PLANT DISEASE LA English DT Article ID POLYMERASE-CHAIN-REACTION; PHAKOPSORA-PACHYRHIZI; SEQUENCE-ANALYSIS; MAIZE; RESISTANCE; ASSAYS; GENE; IDENTIFICATION; INHERITANCE; MORPHOLOGY AB Over the past several years, southern corn rust (SCR) outbreaks caused by the fungus Puccinia polysora have become increasingly problematic for corn growers in the United States. SCR is currently diagnosed through the visual examination of disease symptoms and pathogen morphology, including pigmentation, size, shape, and location of fruiting structures. However, these characteristics are similar to those produced by the common corn rust fungus P. sorghi, confounding accurate visual diagnosis of SCR. Here we report the development of a real-time polymerase chain reaction assay that discriminates between P polysora and P. sorghi. Sequences of the rDNA internal transcribed spacer region were determined for P. polysora and P. sorghi. 5-Carboxyfluorescein fluorophore-labeled hydrolysis probes that differed at 14 nucleotide positions between the species were developed from these data and used to screen DNA extracted directly from rust-infected corn leaves. Species-specific, reproducible identifications of the pathogens were made from as little as 50 pg of DNA within 30 min, and were reliably performed from both recent collections and herbarium specimens. This assay will be useful for rapid and accurate diagnosis of SCR, and could serve as a tool to monitor the distribution and incidence of the disease in the United States. C1 [Crouch, Jo Anne; Szabo, Les J.] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Szabo, LJ (reprint author), Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM les.szabo@ars.usda.gov RI Crouch, Jo Anne/F-4322-2012; OI Crouch, Jo Anne/0000-0001-6886-8090 NR 45 TC 6 Z9 7 U1 4 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 624 EP 632 DI 10.1094/PDIS-10-10-0745 PG 9 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700001 ER PT J AU Smith, JA O'Donnell, K Mount, LL Shin, K Peacock, K Trulock, A Spector, T Cruse-Sanders, J Determann, R AF Smith, Jason A. O'Donnell, Kerry Mount, Lacey L. Shin, Keumchul Peacock, Kelly Trulock, Aaron Spector, Tova Cruse-Sanders, Jenny Determann, Ron TI A Novel Fusarium Species Causes a Canker Disease of the Critically Endangered Conifer, Torreya taxifolia SO PLANT DISEASE LA English DT Article ID FLORIDA TORREYA; PHYLOGENETIC DIVERSITY; RECOGNITION; DECLINE; COMPLEX; TREE; ARN. AB A canker disease of Florida torreya (Torreya taxifolia) has been implicated in the decline of this critically endangered species in its native ranee of northern Florida and southeastern Georgia. In surveys of eight Florida torreya sites, cankers were present on all dead trees and 71 to 100% of living trees, suggesting that a fungal pathogen might be the causal agent. To identify the causal agent, nuclear ribosomal internal transcribed spacer region (ITS rDNA) sequences were determined for 115 fungi isolated from cankers on 46 symptomatic trees sampled at three sites in northern Florida. BLASTn searches of the GenBank nucleotide database, using the ITS rDNA sequences as the query, indicated that a novel Fusarium species designated Fsp-1 might be the etiological agent. Molecular phylogenetic analyses of partial translation elongation factor I-alpha (EF-1) and RNA polymerase second largest subunit (RPB2) gene sequences indicate that Fsp-1 represents a novel species representing one of the earliest divergences within the Gibberella clade of Fusarium. Results of pathogenicity experiments established that the four isolates of Fsp-1 tested could induce canker symptoms on cultivated Florida torreya in a growth chamber. Koch's postulates were completed by the recovery and identification of Fsp-1 from cankers of the inoculated plants. C1 [Smith, Jason A.; Shin, Keumchul; Peacock, Kelly; Trulock, Aaron] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [O'Donnell, Kerry] ARS, Bacterial Food Borne Pathogens & Mycol Res Unit, USDA, Peoria, IL USA. [Mount, Lacey L.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Spector, Tova] Environm Protect Dept, Florida Pk Serv, Panama City, FL USA. [Cruse-Sanders, Jenny; Determann, Ron] Atlanta Bot Garden, Atlanta, GA USA. RP Smith, JA (reprint author), Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM jasons@ufl.edu NR 27 TC 10 Z9 11 U1 3 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 633 EP 639 DI 10.1094/PDIS-10-10-0703 PG 7 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700002 ER PT J AU Zhao, DL Glynn, NC Glaz, B Comstock, JC Sood, S AF Zhao, Duli Glynn, Neil C. Glaz, Barry Comstock, Jack C. Sood, Sushma TI Orange Rust Effects on Leaf Photosynthesis and Related Characters of Sugarcane SO PLANT DISEASE LA English DT Article ID PUCCINIA-KUEHNII; 1ST REPORT; CAUSAL AGENT; BROWN RUST; STOMATAL CLOSURE; GAS-EXCHANGE; WATER-STRESS; BEAN-LEAVES; WHEAT; REGISTRATION AB Orange rust of sugarcane (Saccharum spp. hybrids), caused by Puccinia kuehnii, is a relatively new disease in the Western Hemisphere that substantially reduces yields in susceptible sugarcane genotypes. The objective of this study was to determine the physiological mechanisms of orange rust induced reductions in sugarcane growth and yield by quantifying effects of the disease on leaf SPAD index (an indication of leaf chlorophyll content), net photosynthetic rate, dark respiration, maximum quantum yield of CO2 assimilation, carbon fixation efficiency, and the relationships between these leaf photosynthetic components and rust disease ratings. Plants growing in pots were inoculated with the orange rust pathogen using a leaf whorl inoculation method. A disease rating was assigned using a scale from 0 to 4 with intervals of 0.5. At disease ratings >= 2, the rust-infected leaf portion of inoculated plants showed significant reductions in SPAD index, maximum quantum yield, carbon fixation efficiency, stomatal conductance, leaf transpiration rate, and net photosynthetic rate; but the rusted portion of the infected leaves had increased intercellular CO, concentration and leaf dark respiration rate. Although leaf SPAD index, photosynthetic rate, stomatal conductance, and transpiration rate at the rust-infected portion decreased linearly with increased rust rating, the effect of orange rust on photosynthetic rate was much greater than that on stomatal conductance and transpiration. Unlike earlier reports on other crops, reduction in leaf photosynthesis by orange rust under low light was greater than that under high light conditions. These results help improve the understanding of orange rust etiology and physiological bases of sugarcane yield loss caused by orange rust. C1 [Zhao, Duli; Glynn, Neil C.; Glaz, Barry; Comstock, Jack C.; Sood, Sushma] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Zhao, DL (reprint author), ARS, USDA, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. EM duli.zhao@ars.usda.gov NR 47 TC 16 Z9 17 U1 1 U2 27 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 640 EP 647 DI 10.1094/PDIS-10-10-0762 PG 8 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700003 ER PT J AU Funnell-Harris, DL Pedersen, JF AF Funnell-Harris, Deanna L. Pedersen, Jeffrey F. TI Presence of Fusarium spp. in Air and Soil Associated with Sorghum Fields SO PLANT DISEASE LA English DT Article ID FUJIKUROI SPECIES COMPLEX; SMALL-GRAIN CEREALS; SPORE DEPOSITION; FUNGAL INVASION; SECTION LISEOLA; AIRBORNE SPORES; HEAD BLIGHT; EAR BLIGHT; MAIZE; MOLD AB Sorghum grain, valuable for feed, food, and bioenergy, can be colonized by several Fusarium spp.; therefore, it was of interest to identify possible sources of conidia. Analysis of air and soil samples provided evidence for the presence of propagules from Fusarium genotypes that may cause grain infections. Soil population estimates of members of the Gibberella fujikuroi species complex, that includes sorghum pathogens and other Fusarium spp., suggested that adequate inoculum for systemic infections was present. Conidia in air samples within two sorghum fields were collected by passive trapping for 2 years. Subsampled Fusarium isolates indicated that numbers of G. fujikuroi increased from anthesis through maturity, which coincides with grain development stages vulnerable to Fusarium spp. Genotyping using translation elongation factor 1-alpha gene sequences revealed that spore trap isolates included members of G. fujikuroi that are sorghum pathogens: Fusarium thapsinum, F verticillioides, E proliferatum, and F andiyazi. Also detected were E graminearum, F subglutinans, and several E incarnatum-F equiseti species complex haplotypes that colonize sorghum asymptomatically. All commonly found grain colonizers were detected from air samples in this study. C1 [Funnell-Harris, Deanna L.; Pedersen, Jeffrey F.] Univ Nebraska, GFBRU, USDA, ARS, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Pedersen, Jeffrey F.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RP Funnell-Harris, DL (reprint author), Univ Nebraska, GFBRU, USDA, ARS, Lincoln, NE 68583 USA. EM Deanna.Funnell-Harris@ars.usda.gov NR 61 TC 5 Z9 5 U1 1 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 648 EP 656 DI 10.1094/PDIS-09-10-0671 PG 9 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700004 ER PT J AU Chellemi, DO Webster, CG Baker, CA Annamalai, M Achor, D Adkins, S AF Chellemi, Dan O. Webster, Craig G. Baker, Carlye A. Annamalai, Mani Achor, Diann Adkins, Scott TI Widespread Occurrence and Low Genetic Diversity of Colombian datura virus in Brugmansia Suggest an Anthropogenic Role in Virus Selection and Spread SO PLANT DISEASE LA English DT Article ID 1ST REPORT; MOSAIC-VIRUS; MOTTLE VIRUS; PLANT-VIRUS; RT-PCR; TOMATO; POTYVIRUS; HUNGARY; GERMANY; TOBACCO AB Brugmansia (Brugmansia spp.) is a perennial shrub in the Solanaceae, originating from South America, that is a popular landscape plant in the tropics and subtropics and container plant in temperate regions. Virus-like symptoms including mosaic, rugosity, and faint chlorotic spots were first observed on leaves of Brugmansia plants in a south Florida nursery in November 2003. Colombian datura virus (CDV) was identified in these initial plants and subsequent Brugmansia and Datura metel (a Brugmansia relative also grown as an ornamental) plants obtained from Florida, Connecticut, Wisconsin, and California. Overall, 77.5% of Brugmansia and two of four D. metel plants tested were infected with CDV. Partial NIb/CP sequences of 28 Brugmansia CDV isolates from this study were compared with all 16 CDV isolates in GenBank and found to share high levels of nucleotide and amino acid identity, with negative selection estimated to be occurring. A single Brugmansia plant was also infected with a recently described tobamovirus. The low genetic diversity of CDV observed, along with negative selection pressure on NIb/CP, suggests a recent ancestry (<400 years) of the worldwide population of CDV, coinciding with anthropogenic collection and dissemination of Brugmansia plants from their center of origin. C1 [Chellemi, Dan O.; Webster, Craig G.; Adkins, Scott] ARS, USDA, Ft Pierce, FL 34945 USA. [Baker, Carlye A.; Annamalai, Mani] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA. [Achor, Diann] Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. RP Adkins, S (reprint author), ARS, USDA, Ft Pierce, FL 34945 USA. EM scott.adkins@ars.usda.gov NR 49 TC 6 Z9 7 U1 0 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 755 EP 761 DI 10.1094/PDIS-09-10-0654 PG 7 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700019 ER PT J AU Schilder, AMC Lizotte, EM Yun, HY Dixon, LJ Castlebury, LA AF Schilder, A. M. C. Lizotte, E. M. Yun, H. Y. Dixon, L. J. Castlebury, L. A. TI First Report of Juneberry Rust Caused by Gymnosporangium nelsonii on Juneberry in Michigan SO PLANT DISEASE LA English DT News Item C1 [Schilder, A. M. C.] Michigan State Univ, Dept Plant Pathol, E Lansing, MI 48824 USA. [Lizotte, E. M.] Michigan State Univ Extens, NW Michigan Hort Res Ctr, Traverse City, MI 49684 USA. [Yun, H. Y.; Dixon, L. J.; Castlebury, L. A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. RP Schilder, AMC (reprint author), Michigan State Univ, Dept Plant Pathol, E Lansing, MI 48824 USA. NR 4 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 770 EP 771 DI 10.1094/PDIS-12-10-0874 PG 2 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700031 ER PT J AU Rebollar-Alviter, A Minnis, AM Dixon, LJ Castlebury, LA Ramirez-Mendoza, MR Silva-Rojas, HV Valdovinos-Ponce, G AF Rebollar-Alviter, A. Minnis, A. M. Dixon, L. J. Castlebury, L. A. Ramirez-Mendoza, M. R. Silva-Rojas, H. V. Valdovinos-Ponce, G. TI First Report of Leaf Rust of Blueberry Caused by Thekopsora minima in Mexico SO PLANT DISEASE LA English DT News Item C1 [Rebollar-Alviter, A.] Univ Autonoma Chapingo, Ctr Reg Morelia, Morelia, Michoacan, Mexico. [Minnis, A. M.; Dixon, L. J.; Castlebury, L. A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD USA. [Ramirez-Mendoza, M. R.] Parasitol Agr UACh, Chapingo, Mexico. [Silva-Rojas, H. V.] Lab Biotecnol Semillas, Montecillo, Mexico. [Valdovinos-Ponce, G.] Inst Fitosanidad, Montecillo, Mexico. RP Rebollar-Alviter, A (reprint author), Univ Autonoma Chapingo, Ctr Reg Morelia, Morelia, Michoacan, Mexico. OI Rebollar-Alviter, Angel/0000-0002-6641-456X NR 4 TC 1 Z9 2 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2011 VL 95 IS 6 BP 772 EP 772 DI 10.1094/PDIS-12-10-0885 PG 1 WC Plant Sciences SC Plant Sciences GA 767TN UT WOS:000290879700035 ER PT J AU Ueno, D Milner, MJ Yamaji, N Yokosho, K Koyama, E Zambrano, MC Kaskie, M Ebbs, S Kochian, LV Ma, JF AF Ueno, Daisei Milner, Matthew J. Yamaji, Naoki Yokosho, Kengo Koyama, Emi Zambrano, M. Clemencia Kaskie, Molly Ebbs, Stephen Kochian, Leon V. Ma, Jian Feng TI Elevated expression of TcHMA3 plays a key role in the extreme Cd tolerance in a Cd-hyperaccumulating ecotype of Thlaspi caerulescens SO PLANT JOURNAL LA English DT Article DE cadmium; tolerance; hyperaccumulator; vacuole; transporter ID HEAVY-METAL HYPERACCUMULATION; 2 CONTRASTING ECOTYPES; ARABIDOPSIS-HALLERI; CELLULAR COMPARTMENTATION; CADMIUM ACCUMULATION; TRANSPORTER GENES; ZINC TOLERANCE; ATPASE; THALIANA; LEAVES AB P>Cadmium (Cd) is a highly toxic heavy metal for plants, but several unique Cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. However, the molecular mechanisms underlying this hypertolerance to Cd are poorly understood. Here we have isolated and functionally characterized an allelic gene, TcHMA3 (heavy metal ATPase 3) from two ecotypes (Ganges and Prayon) of Thlaspi caerulescens contrasting in Cd accumulation and tolerance. The TcHMA3 alleles from the higher (Ganges) and lower Cd-accumulating ecotype (Prayon) share 97.8% identity, and encode a P(1B)-type ATPase. There were no differences in the expression pattern, cell-specificity of protein localization and transport substrate-specificity of TcHMA3 between the two ecotypes. Both alleles were characterized by constitutive expression in the shoot and root, a tonoplast localization of the protein in all leaf cells and specific transport activity for Cd. The only difference between the two ecotypes was the expression level of TcHMA3: Ganges showed a sevenfold higher expression than Prayon, partly caused by a higher copy number. Furthermore, the expression level and localization of TcHMA3 were different from AtHMA3 expression in Arabidopsis. Overexpression of TcHMA3 in Arabidopsis significantly enhanced tolerance to Cd and slightly increased tolerance to Zn, but did not change Co or Pb tolerance. These results indicate that TcHMA3 is a tonoplast-localized transporter highly specific for Cd, which is responsible for sequestration of Cd into the leaf vacuoles, and that a higher expression of this gene is required for Cd hypertolerance in the Cd-hyperaccumulating ecotype of T. caerulescens. C1 [Milner, Matthew J.; Kochian, Leon V.] Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Ueno, Daisei; Yamaji, Naoki; Yokosho, Kengo; Koyama, Emi; Ma, Jian Feng] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan. [Milner, Matthew J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Zambrano, M. Clemencia; Kaskie, Molly; Ebbs, Stephen] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA. RP Kochian, LV (reprint author), Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM lvk1@cornell.edu; maj@rib.okayama-u.ac.jp RI YAMAJI, Naoki/B-2524-2011; Ma, Jian Feng/C-7213-2009; Milner, Matthew/M-2430-2014; OI YAMAJI, Naoki/0000-0002-7499-3004; Kochian, Leon/0000-0003-3416-089X; Milner, Matthew/0000-0001-9184-7423; Ebbs, Stephen/0000-0003-4042-9234 FU Ministry of Education, Culture, Sports, Science and Technology of Japan [21248009, 22119002]; USDA Agricultural Research Service; National Science Foundation [0346276] FX We thank Pierre Richaud group for kindly giving us the AtHMA3w cDNA. This research was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (no. 21248009 and 22119002 to JFM), by funding from the USDA Agricultural Research Service (to LVK) and by the National Science Foundation (no. 0346276 to SE). NR 55 TC 80 Z9 84 U1 5 U2 59 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0960-7412 J9 PLANT J JI Plant J. PD JUN PY 2011 VL 66 IS 5 BP 852 EP 862 DI 10.1111/j.1365-313X.2011.04548.x PG 11 WC Plant Sciences SC Plant Sciences GA 768QY UT WOS:000290952900012 PM 21457363 ER PT J AU Santin, M Fayer, R AF Santin, Monica Fayer, Ronald TI Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animals SO RESEARCH IN VETERINARY SCIENCE LA English DT Review DE Enterocytozoon bieneusi; Microsporidia; Genotypes; ITS ID EASTERN UNITED-STATES; VIRUS-INFECTED PATIENTS; RIBOSOMAL-RNA SEQUENCE; ENCEPHALITOZOON-CUNICULI; FECAL SAMPLES; 1ST DETECTION; INTESTINAL MICROSPORIDIOSIS; MOLECULAR CHARACTERIZATION; MONOCLONAL-ANTIBODIES; SERIAL PROPAGATION AB Microsporidia are a ubiquitous group of obligate intracellular parasites that infect all major animal groups. Enterocytozoon bieneusi is the most commonly identified Microsporidia in humans and has also been reported worldwide in animals with importance in veterinary medicine (e.g. cats, dogs, horses, cattle and pigs). The identification of E. bieneusi in animals has raised the question of the importance of animal reservoirs in the epidemiology of this pathogen, and the implications of the infection with this pathogen in infected animals. Considerable genetic diversity within E. bieneusi has been found with over 90 genotypes identified based on the ITS nucleotide sequence of E. bieneusi spores recovered from the feces of infected humans and animals. Both host-adapted E. bieneusi genotypes with narrow host ranges and potentially zoonotic genotypes with wide host specificity have been identified. The information presented in this review should be useful in understanding the taxonomy, epidemiology, zoonotic potential, and importance in public health of E. bieneusi. Published by Elsevier Ltd. C1 [Santin, Monica; Fayer, Ronald] ARS, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. RP Santin, M (reprint author), ARS, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, USDA, Bldg 173,BARC E,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov NR 81 TC 96 Z9 100 U1 1 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD JUN PY 2011 VL 90 IS 3 BP 363 EP 371 DI 10.1016/j.rvsc.2010.07.014 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 770KK UT WOS:000291085200002 PM 20699192 ER PT J AU Szabo, DT Diliberto, JJ Hakk, H Huwe, JK Birnbaum, LS AF Szabo, David Taylor Diliberto, Janet J. Hakk, Heldur Huwe, Janice K. Birnbaum, Linda S. TI Toxicokinetics of the Flame Retardant Hexabromocyclododecane Alpha: Effect of Dose, Timing, Route, Repeated Exposure, and Metabolism SO TOXICOLOGICAL SCIENCES LA English DT Article DE toxicokinetics; biotransformation; lipophilic; persistent organic pollutants; endocrine disruptors; risk assessment; brominated flame retardant; hexabromocyclododecane; mixture; metabolism ID POLYBROMINATED DIPHENYL ETHERS; CYP1A2-KNOCKOUT; BREAST-MILK; WISTAR RATS; MICE; HBCDS; GAMMA; ACCUMULATION; C57BL/6N; BEHAVIOR AB Alpha-hexabromocyclododecane (alpha-HBCD) is an emerging persistent organic pollutant present in the hexabromocyclododecane (HBCD) commercial mixture. HBCD is used as an additive flame retardant in a wide variety of household consumer products. Three main stereoisomers, alpha (alpha), beta (beta), and gamma (gamma), comprise roughly 10, 10, and 80% of the mixture, respectively. Despite its small contribution to HBCD global production and usage, alpha-HBCD is the major stereoisomer found in wildlife and human tissues including breast milk and blood in North America, European Union, and Asia. No mammalian or human data are currently available regarding the toxicokinetics of alpha-HBCD. This study was conducted in an effort to fully characterize the absorption, distribution, metabolism, and elimination of alpha-HBCD following a single and repeated exposure with respect to dose, time, and route of administration in female C57BL/6 mice. Results indicate that similar to 90% of the administered dose (3 mg/kg) was absorbed after oral exposure. Disposition was (1) dictated by lipophilicity, as adipose, liver, muscle, and skin were major depots and (2) was dose dependent with nonlinear accumulation at higher doses. Elimination, both whole-body and from individual tissues, was biphasic. alpha-HBCD-derived radioactivity was excreted in the feces as parent and metabolites, whereas urine only contained metabolites. Presence of polar metabolites in the blood and urine were a major factor in determining the rapid initial whole-body half-life after a single oral exposure. Initial half-lives were similar to 1-3 days and much longer terminal half-lives of 17 days were observed, suggesting the potential for alpha-HBCD bioaccumulation. A 10-day repeated study supports alpha-HBCD bioaccumulation potential. Stereoisomerization previously observed after exposure to gamma-HBCD was not seen after exposure of alpha-HBCD. The toxicokinetic behavior reported here has important implications for the extrapolation of toxicological studies of the commercial HBCD mixture to the assessment of risk of alpha-HBCD which is the major stereoisomer found in wildlife and people. C1 [Szabo, David Taylor; Diliberto, Janet J.] US EPA, Off Res & Dev, Natl Hlth Effects & Exposure Res Lab, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA. [Szabo, David Taylor] Univ N Carolina, Curriculum Toxicol, Res Triangle Pk, NC 27711 USA. [Hakk, Heldur; Huwe, Janice K.] Agr Res Serv, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Birnbaum, Linda S.] NCI, Res Triangle Pk, NC 27709 USA. [Birnbaum, Linda S.] Natl Inst Environm Hlth Sci, NIH, Res Triangle Pk, NC 27709 USA. RP Szabo, DT (reprint author), US EPA, Off Res & Dev, Natl Hlth Effects & Exposure Res Lab, Integrated Syst Toxicol Div, Mail Drop B143-01,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM szabo.david@epa.gov FU University of North Carolina in Chapel Hill; Environmental Protection Agency [CR 833237] FX This work is funded in part by a cooperative agreement between the University of North Carolina in Chapel Hill and the Environmental Protection Agency (CR 833237) predoctoral training grants. NR 38 TC 21 Z9 21 U1 3 U2 49 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 J9 TOXICOL SCI JI Toxicol. Sci. PD JUN PY 2011 VL 121 IS 2 BP 234 EP 244 DI 10.1093/toxsci/kfr059 PG 11 WC Toxicology SC Toxicology GA 768JS UT WOS:000290931000002 PM 21441408 ER PT J AU Marini, JC Lanpher, BC Scaglia, F O'Brien, WE Sun, Q Garlick, PJ Jahoor, F Lee, B AF Marini, Juan C. Lanpher, Brendan C. Scaglia, Fernando O'Brien, William E. Sun, Qin Garlick, Peter J. Jahoor, Farook Lee, Brendan TI Phenylbutyrate improves nitrogen disposal via an alternative pathway without eliciting an increase in protein breakdown and catabolism in control and ornithine transcarbamylase-deficient patients SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID UREA CYCLE DISORDERS; SODIUM PHENYLBUTYRATE; HUMANS; PHENYLACETATE; METABOLISM; LEUCINE; ACID; PHENYLACETYLGLUTAMINE; THERAPY; INHIBITION AB Background: Phenylbutyrate is a drug used in patients with urea cycle disorder to elicit alternative pathways for nitrogen disposal. However, phenylbutyrate administration decreases plasma branched-chain amino acid (BCAA) concentrations, and previous research suggests that phenylbutyrate administration may increase leucine oxidation, which would indicate increased protein degradation and net protein loss. Objective: We investigated the effects of phenylbutyrate administration on whole-body protein metabolism, glutamine, leucine, and urea kinetics in healthy and ornithine transcarbamylase-deficient (OTCD) subjects and the possible benefits of BCAA supplementation during phenylbutyrate therapy. Design: Seven healthy control and 7 partial-OTCD subjects received either phenylbutyrate or no treatment in a crossover design. In addition, the partial-OTCD and 3 null-OTCD subjects received phenylbutyrate and phenylbutyrate plus BCAA supplementation. A multitracer protocol was used to determine the whole-body fluxes of urea and amino acids of interest. Results: Phenylbutyrate administration reduced ureagenesis by approximate to 15% without affecting the fluxes of leucine, tyrosine, phenylalanine, or glutamine and the oxidation of leucine or phenylalanine. The transfer of N-15 from glutamine to urea was reduced by 35%. However, a reduction in plasma concentrations of BCAAs due to phenylbutyrate treatment was observed. BCAA supplementation did not alter the respective baseline fluxes. Conclusions: Prolonged phenylbutyrate administration reduced ureagenesis and the transfer of N-15 from glutamine to urea without parallel reductions in glutamine flux and concentration. There were no changes in total-body protein breakdown and amino acid catabolism, which suggests that phenylbutyrate can be used to dispose of nitrogen effectively without adverse effects on body protein economy. Am J Clin Nutr 2011;93:1248-54. C1 [Lanpher, Brendan C.; Scaglia, Fernando; O'Brien, William E.; Sun, Qin; Lee, Brendan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Marini, Juan C.; Jahoor, Farook] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Lee, Brendan] Univ Illinois, Howard Hughes Med Inst, Champaign, IL 61820 USA. [Garlick, Peter J.] Univ Illinois, Dept Anim Sci, Champaign, IL 61820 USA. RP Lee, B (reprint author), Baylor Coll Med, Dept Mol & Human Genet, 1 Baylor Plaza,Room R814, Houston, TX 77030 USA. EM blee@bcm.edu FU Ucyclyd Pharma Inc.; NIH [DK54450, RR024173, HD061221]; Baylor College of Medicine General Clinical Research Center; Texas Medical Center Digestive Disease Center; Urea Cycle Disorders Rare Disease Clinical Research Network; National Urea Cycle Disorders Foundation FX The authors' responsibilities were as follows-JCM: designed the study, analyzed samples and data, and prepared the report; BCL and FS: implemented the study and prepared the report; WEO and QS: analyzed samples and data; PJG: designed the study and prepared the report; FJ: designed the study, analyzed samples, and prepared the report; and BL: designed the study, analyzed data, and prepared the report. BL received research grant support from Ucyclyd Pharma Inc. JCM, BCL, FS, WEO, QS, PJG, and FJ received no financial support from nor had any personal interest in any company or organization that sponsored the research.; Supported by the NIH (DK54450 to BL; RR024173 to JCM), the Baylor College of Medicine General Clinical Research Center, the Texas Medical Center Digestive Disease Center, the Urea Cycle Disorders Rare Disease Clinical Research Network, NIH (HD061221 to BL), and the National Urea Cycle Disorders Foundation. NR 25 TC 4 Z9 4 U1 0 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2011 VL 93 IS 6 BP 1248 EP 1254 DI 10.3945/ajcn.110.009043 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 766PD UT WOS:000290796700011 PM 21490144 ER PT J AU Bhupathiraju, SN Tucker, KL AF Bhupathiraju, Shilpa N. Tucker, Katherine L. TI The implication of unknown bioactive compounds and cooking techniques in relations between the variety in fruit and vegetable intake and inflammation Reply SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Letter ID MARKERS C1 [Bhupathiraju, Shilpa N.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. RP Bhupathiraju, SN (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. EM kl.tucker@neu.edu RI Tucker, Katherine/A-4545-2010; Bhupathiraju, Shilpa/I-1209-2013 NR 9 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC CLINICAL NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, SUBSCRIPTIONS, RM L-3300, BETHESDA, MD 20814-3998 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2011 VL 93 IS 6 BP 1384 EP 1385 DI 10.3945/ajcn.111.013680 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 766PD UT WOS:000290796700029 ER PT J AU Tarkalson, DD King, BA Bjorneberg, DL Taberna, JP AF Tarkalson, David D. King, Bradley A. Bjorneberg, Dave L. Taberna, John P., Jr. TI Evaluation of In-Row Plant Spacing and Planting Configuration for Three Irrigated Potato Cultivars SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Russet Burbank; Russet Norkotah; Ranger Russet; Bed planting ID RUSSET BURBANK POTATOES; PROCESSING QUALITY; TUBER-SIZE; YIELD; POPULATION; MANITOBA; TILLAGE; DENSITY; DATE; STEM AB Research studies have shown that planting potatoes (Solanum tuberosum L.) in a bed configuration can improve water movement into the potato root zone. However, plant spacing recommendations are needed for potatoes planted in a bed configuration. This study was conducted to evaluate the effect of in-row plant spacing and planting configuration on yield of Russet Burbank, Russet Norkotah, and Ranger Russet potatoes under sprinkler irrigation. For the three cultivars, the effect of in-row plant spacing (three spacing treatments) for each planting configuration (4 row conventional ridged-row [4RC], 5 row bed [5RB], and 7 row bed [7RB]), and the effect of planting configuration at a uniform population on total tuber yield, U.S. No. 1 tuber yield, tubers per ha, average size (by weight), and large tuber yield were investigated at the USDA-ARS Northwest Irrigation & Soils Research Lab in Kimberly, ID on a Portneuf silt loam (coarse-silty mixed mesic Durixerollic Calciorthid) in 2008 and 2009. The greatest influence of in-row plant spacing was on average size and tubers per ha. In general, as in-row plant spacing increased (plant population decreased) the average tuber size increased and tubers per ha decreased. There was little influence of in-row spacing on measured production variables under the bed planting configurations except for tubers per ha which generally increased with narrower plant spacing. For Russet Norkotah and Ranger Russet there were few differences in measured production variables between planting configuration treatments. For Russet Burbank, the 4RC planting configuration had 14.6% significantly greater total tuber yield than the 7RB planting configuration, 20.2% greater U.S. No. 1 tuber yield than both bed planting configurations, and 25.2 and 29.9% greater large tuber yield than the 5RB and 7RB planting configurations, in 2009. Optimum production of Russet Norkotah and Ranger Russet potatoes is possible under all the planting configurations and plant spacing range evaluated in this study, granting growers flexibility in their systems, however, evidence from this study suggests that production of Russet Burbank may be less suited to bed planting configurations. C1 [Tarkalson, David D.; King, Bradley A.; Bjorneberg, Dave L.] ARS, USDA, NW Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Taberna, John P., Jr.] Western Ag Res LLC, Blackfoot, ID 83221 USA. RP Tarkalson, DD (reprint author), ARS, USDA, NW Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. EM david.tarkalson@ars.usda.gov NR 29 TC 6 Z9 7 U1 4 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2011 VL 88 IS 3 BP 207 EP 217 DI 10.1007/s12230-010-9185-9 PG 11 WC Agronomy SC Agriculture GA 768DU UT WOS:000290912500002 ER PT J AU Jansky, SH Hamernik, A Bethke, PC AF Jansky, Shelley H. Hamernik, Andy Bethke, Paul C. TI Germplasm Release: Tetraploid Clones with Resistance to Cold-Induced Sweetening SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato chip processing; Solanum; Wild germplasm; Low temperature sweetening; Reducing sugars ID CHIPPING GENOTYPES; CULTIVATED POTATO; SELECTION; TUBERS; GLYCOALKALOIDS; STORAGE AB Long-term cold storage is necessary to supply potatoes to the processing industry throughout the year. Cold-induced sweetening prevents current potato cultivars from producing acceptable chips after cold storage. Resistance to cold-induced sweetening has been introgressed into the cultivated potato from wild Solanum relatives. This paper describes five tetraploid interspecific hybrid clones that can be used in breeding programs to improve chip quality in cold-stored tubers. The clones are male and female fertile, and are adapted to temperate zone production environments. All clones except M1 have low levels of glycoalkaloids. They produce acceptable chip color following storage at 4.4A degrees C for 3 months. Tubers from all clones have high specific gravity. Following cold storage, tuber glucose and fructose contents are low compared to standard cultivars. Tuber sucrose contents for the five clones are either comparable to that in standard cultivars or much greater. C1 [Jansky, Shelley H.; Hamernik, Andy; Bethke, Paul C.] ARS, USDA, Madison, WI 53706 USA. [Jansky, Shelley H.; Hamernik, Andy; Bethke, Paul C.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. RP Jansky, SH (reprint author), ARS, USDA, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 22 TC 6 Z9 6 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2011 VL 88 IS 3 BP 218 EP 225 DI 10.1007/s12230-011-9186-3 PG 8 WC Agronomy SC Agriculture GA 768DU UT WOS:000290912500003 ER PT J AU Brown, CR Haynes, KG Moore, M Pavek, MJ Hane, DC Love, SL Novy, RG Miller, JC AF Brown, Charles Raymond Haynes, Kathleen G. Moore, Martin Pavek, Mark J. Hane, Daniel C. Love, Steven L. Novy, Richard G. Miller, J. Creighton, Jr. TI Stability and Broad-sense Heritability of Mineral Content in Potato: Zinc SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE ICAEPS; Solanum tuberosum; Zinc content genotype x environment interaction; Stability analysis; Zinc deficiency disease ID HUMAN-NUTRITION PERSPECTIVE; MICRONUTRIENT MALNUTRITION; CULTIVARS; PLANTS; CROPS; IRON; FOOD AB The mineral content of potato is an important consideration in the evaluation of its role in the human diet. Zinc content is vital due to its crucial role as a micronutrient. Zinc deficiency occurs among the poorest of the world's populations. In this study, 36 breeding lines and varieties (genotypes) were divided among three trials (Tri-State, Western Regional Russet and Western Specialty/Red) which were grown in 11 locations. Zinc content was measured in harvested tubers by wet ashing and passage through an Inductively Coupled Argon Plasma Emission Spectrophotometer Genotype mean zinc content ranged from 12 to 18 mu g g(-1) dry weight over all trials. In two of the three trials there were no significant differences among genotypes for zinc. Broad sense heritabilities for zinc content were small in these two trials. In the Western Regional Russet Trial there were significant differences among genotypes and the heritability was 0.61, suggesting that genotypes with higher zinc content could be selected. However, the largest zinc value was only 50% above the lowest value. Furthermore, a 100 g serving of the highest zinc genotype would only provide 4% of the adult Estimated Average Requirement From these results, potato from this breeding pool would not appear to be a good candidate for biofortification of zinc through traditional breeding. Higher values from other studies suggest that zinc biofortification through breeding may be warranted in potato for populations with high potato consumption and high risk for zinc deficiency in the Andes of South America. C1 [Brown, Charles Raymond] ARS, USDA, Prosser, WA 99350 USA. [Haynes, Kathleen G.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Moore, Martin] Agri NW, Plymouth, WA 99346 USA. [Pavek, Mark J.] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Hane, Daniel C.] Oregon State Univ, Dept Crop Sci, HAREC, Hermiston, OR 97838 USA. [Love, Steven L.] Univ Idaho, AREC, Aberdeen, ID 83210 USA. [Novy, Richard G.] ARS, USDA, Aberdeen, ID 83210 USA. [Miller, J. Creighton, Jr.] Texas A&M Univ, Dept Hort, College Stn, TX 77843 USA. RP Brown, CR (reprint author), ARS, USDA, Prosser, WA 99350 USA. EM chuck.brown@ars.usda.gov NR 33 TC 9 Z9 10 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2011 VL 88 IS 3 BP 238 EP 244 DI 10.1007/s12230-011-9188-1 PG 7 WC Agronomy SC Agriculture GA 768DU UT WOS:000290912500005 ER PT J AU Whitworth, JL Novy, RG Stark, JC Pavek, JJ Corsini, DL Love, SL Olsen, N Gupta, SK Brandt, T Vales, MI Mosley, AR Yilma, S James, SR Hane, DC Charlton, BA Shock, CC Knowles, NR Pavek, MJ Miller, JS Brown, CR AF Whitworth, Jonathan L. Novy, Richard G. Stark, Jeffrey C. Pavek, Joseph J. Corsini, Dennis L. Love, Steven L. Olsen, Nora Gupta, Sanjay K. Brandt, Tina Vales, M. Isabel Mosley, Alvin R. Yilma, Solomon James, Steve R. Hane, Dan C. Charlton, Brian A. Shock, Clinton C. Knowles, N. Richard Pavek, Mark J. Miller, Jeffrey S. Brown, Charles R. TI Alpine Russet: A Potato Cultivar Having Long Tuber Dormancy Making it Suitable for Processing from Long-term Storage SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Tuber dormancy; Breeding; French fry ID DRY ROT; RESISTANCE; QUALITY; VARIETY AB Alpine Russet is a later maturing, oblong-long, lightly russeted potato cultivar, notable for having tuber dormancy comparable to Russet Burbank. Processing quality of Alpine Russet from long-term storage is superior to Russet Burbank, with low percent reducing sugars and uniform fry color due to a low percentage difference of sugars between the bud and stem ends. Alpine Russet yields were comparable to Russet Burbank in early harvest trials and were comparable or significantly larger in late harvest trials depending on the location. At two late season locations, Alpine Russet had the largest total and percent No. 1 yields and the largest percent mid-range No. 1 tubers compared to Ranger Russet and Russet Burbank. It has moderately high specific gravity and is resistant to most external and internal defects. Alpine Russet has been evaluated in public and industry trials throughout the Western U.S. for over 15 years. C1 [Whitworth, Jonathan L.; Novy, Richard G.; Pavek, Joseph J.; Corsini, Dennis L.] ARS, R&E Ctr, USDA, Aberdeen, ID 83210 USA. [Stark, Jeffrey C.; Love, Steven L.] Univ Idaho, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. [Olsen, Nora; Gupta, Sanjay K.; Brandt, Tina] Univ Idaho, Kimberly R&E Ctr, Kimberly, ID 83341 USA. [Vales, M. Isabel; Mosley, Alvin R.; Yilma, Solomon] Oregon State Univ, Corvallis, OR 97331 USA. [James, Steve R.] Oregon State Univ, Madras, OR 97741 USA. [Hane, Dan C.] Oregon State Univ, Hermiston R&E Ctr, Hermiston, OR 97838 USA. [Charlton, Brian A.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Shock, Clinton C.] Oregon State Univ, Malhuer Expt Stn, Ontario, OR 97914 USA. [Knowles, N. Richard; Pavek, Mark J.] Washington State Univ, Pullman, WA 99164 USA. [Miller, Jeffrey S.] Miller Res, Rupert, ID 83350 USA. [Brown, Charles R.] ARS, USDA, Prosser, WA 99350 USA. RP Whitworth, JL (reprint author), ARS, R&E Ctr, USDA, Aberdeen, ID 83210 USA. EM Jonathan.Whitworth@ars.usda.gov FU USDA/CSREES FX The authors thank Margaret Bain, Mel Chappell, Lorie Ewing, Nora Fuller, Mark Fristad, Darren Hall, Charlene Miller, Tom Salaiz, Brian Schneider, Lura Schroeder, Penny Tubbs, Steven Wheeler, and Jim Whitmore, as well as our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Oregon and Washington Potato Commissions for their contributions to the development and release of Alpine Russet. We also express thanks to Dave Hammond from Lamb Weston, Kennewick, WA as well as our other industry cooperators for their substantial contributions to this research effort. Development of Alpine Russet was partially funded by the USDA/CSREES Special Potato Program Grant. NR 12 TC 5 Z9 5 U1 2 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2011 VL 88 IS 3 BP 256 EP 268 DI 10.1007/s12230-011-9190-7 PG 13 WC Agronomy SC Agriculture GA 768DU UT WOS:000290912500007 ER PT J AU Suttle, JC Huckle, LL Lulai, EC AF Suttle, Jeffrey C. Huckle, Linda L. Lulai, Edward C. TI The Effects of Dormancy Status on the Endogenous Contents and Biological Activities of Jasmonic Acid, N-(jasmonoyl)-Isoleucine, and Tuberonic Acid in Potato Tubers SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Dormancy; Jasmonic acid; N-Jasmonoyl-Isoleucine; Tuber; Tuberonic acid; Oxylipin; Solanum tuberosum L.; Sprouting ID SOLANUM-TUBEROSUM; METHYL JASMONATE; MICROTUBER DORMANCY; ABSCISIC-ACID; IN-VITRO; BREAKING; PLANTS; GERMINATION; PROGRESSION; ACTIVATION AB The effects of storage and dormancy progression on the endogenous contents and the growth-regulating activities of jasmonic acid (JA), jasmonoyl-isoleucine (JA-Ile), and tuberonic acid (TA) were determined in potato (Solanum tuberosum L. cv. Russet Burbank) minitubers and seed tubers over several harvest/storage seasons. In apical discs (consisting of both periderm and buds) isolated from minitubers undergoing natural dormancy progression, JA content was low immediately after harvest, remained essentially constant as dormancy weakened, rose > 4-fold as sprouting commenced and declined by > 50% as sprouting became more vigorous. JA-Ile content was more variable; remaining constant over 7 months of storage in 1 year and increasing ca. 5-fold over the same period during a second year. The TA content of minituber apical discs exceeded that of JA or JA-Ile by > 20-fold and declined steadily to ca. 50% of initial levels during storage and dormancy progression. A similar but more temporally compressed pattern was found in chemically forced minituber apical discs following a 24 h treatment with the synthetic dormancy terminating agent bromoethane. A different pattern was observed in meristems isolated from seed tubers at three stages of dormancy progression. JA content was low in dormant meristems and remained constant in meristems isolated at late dormancy and following dormancy exit. The content of JA-Ile rose gradually as meristems progressed from deep dormancy to active sprouting. The TA content of isolated meristems was > 20-fold higher than either JA or JA-Ile and rose slightly during dormancy progression. Exogenous JA (0.001 to 1 mM) had no effect on sprout growth when applied to intact non-dormant minitubers but treatment with 1 mM JA-Ile inhibited sprout growth by 35%. In contrast, direct application of 10 mu g JA to single-eye tissue cylinders resulted in a 29% inhibition of sprout growth after 2 weeks while JA-Ile had no effect. Treatment of dormant minitubers with either JA or JA-Ile (0.01to1 mM) had no effect on dormancy duration or subsequent sprout growth. Collectively, these data do not support a major role for JA or its metabolites JA-Ile and TA in potato tuber dormancy control but they do not exclude other roles for these hormones in tuber physiology and early sprout growth. C1 [Suttle, Jeffrey C.; Huckle, Linda L.; Lulai, Edward C.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Suttle, JC (reprint author), ARS, USDA, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM jeff.suttle@ars.usda.gov NR 41 TC 4 Z9 4 U1 4 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2011 VL 88 IS 3 BP 283 EP 293 DI 10.1007/s12230-011-9192-5 PG 11 WC Agronomy SC Agriculture GA 768DU UT WOS:000290912500009 ER PT J AU Wang, XG Johnson, MW Yokoyama, VY Pickett, CH Daane, KM AF Wang, Xin-geng Johnson, Marshall W. Yokoyama, Victoria Y. Pickett, Charles H. Daane, Kent M. TI Comparative evaluation of two olive fruit fly parasitoids under varying abiotic conditions SO BIOCONTROL LA English DT Article DE Bactrocera oleae; Psyttalia concolor; Psyttalia humilis; Psyttalia lounsburyi; Classical biological control; Field-cage evaluation; Host specificity; Climatic adaptability ID BACTROCERA-OLEAE DIPTERA; PSYTTALIA-CONCOLOR HYMENOPTERA; SPECIES COMPLEX HYMENOPTERA; BIOLOGICAL-CONTROL AGENTS; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; CERATITIS-CAPITATA; ARTHROPOD PESTS; OPIUS-CONCOLOR; TEPHRITIDAE AB Psyttalia lounsburyi (Silvestri) and P. humilis (Silvestri) (Hymenoptera: Braconidae) were evaluated in California for their potential to control the invasive olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae). Psyttalia lounsburyi is a specialist on B. oleae while P. humilis also attacks other tephritid species. Field cage trials, conducted from 2006 to 2009, were used to compare P. lounsburyi and two populations of P. humilis (Kenya and Namibia) in California's interior valley and coastal regions. Both parasitoid species reproduced on B. oleae in all trials. Under similar abiotic conditions, offspring production per female was higher in P. humilis than in P. lounsburyi, suggesting that host specificity by P. lounsburyi does not confer a higher efficiency on B. oleae in cultivated olives. Two abiotic factors were shown to impact parasitoid efficiency. First, adult parasitoid survival was poor during periods of high summer temperatures, common to the olive production areas in California's interior valleys. Second, parasitism levels were lower on B. oleae larvae feeding in larger Ascolano cv. fruit than in smaller Manzanillo cv. fruit. Results are discussed relative to biological control of B. oleae in commercial olives and the usefulness of natural enemies specialized to attack fruit flies in wild olives compared with the larger cultivated olive fruit. C1 [Wang, Xin-geng; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Johnson, Marshall W.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Yokoyama, Victoria Y.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Pickett, Charles H.] Calif Dept Food & Agr, Biol Control Unit, Sacramento, CA 95832 USA. RP Daane, KM (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM xgwang@uckac.edu; mjohnson@uckac.edu; Victoria.yokoyama@ars.usda.gov; cpickett@cdfa.ca.gov; daane@uckac.edu FU California Specialty Crop Block Grant; California Olive Committee; USDA APHIS; CDFA; USDA CSREES FX We are grateful to Martha Gerik (University of California, Riverside) for assistance in all field tests; David Headrick, Pete Peterson and Therese Kapaun (California Polytechnic State University, San Luis Obispo) for facilitating field studies in San Luis Obispo; Walt French, Scott Ritterbuck, Anne May and David Righetti for allowing us to use their olive trees in San Luis Obispo; Arnaud Blanchet and Walker Jones (USDA ARS European Biological Control Laboratory, Montferrier, France) and Pedro Rendon (USDA-APHID-PPQ, Moscamed biological control laboratory, San Miguel Petapa, Guatemala) for providing the initial colonies of parasitoids; John Andrews (University of California, Berkeley) for managing the quarantine importation; and Mathew Middleton (University of California, Berkeley) for molecular analysis of parasitoid colonies. Funds were provided by the California Specialty Crop Block Grant, California Olive Committee, USDA APHIS and CDFA Biological Control Program, and USDA CSREES Special Grants Program: Pest Management Alternatives to MWJ and KMD. NR 50 TC 17 Z9 17 U1 1 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 J9 BIOCONTROL JI Biocontrol PD JUN PY 2011 VL 56 IS 3 BP 283 EP 293 DI 10.1007/s10526-010-9332-8 PG 11 WC Entomology SC Entomology GA 768EI UT WOS:000290913900005 ER PT J AU Seagraves, MP Kajita, Y Weber, DC Obrycki, JJ Lundgren, JG AF Seagraves, Michael P. Kajita, Yukie Weber, Donald C. Obrycki, John J. Lundgren, Jonathan G. TI Sugar feeding by coccinellids under field conditions: the effects of sugar sprays in soybean SO BIOCONTROL LA English DT Article DE Anthrone; Aphis glycines; Conservation biological control; Food spray; Omnivory; Non-prey foods ID COLEOMEGILLA-MACULATA COLEOPTERA; LADY BEETLES COLEOPTERA; SEPTEMPUNCTATA COLEOPTERA; HARMONIA-AXYRIDIS; FOOD-CONSUMPTION; APHID HEMIPTERA; ALFALFA FIELDS; UTAH ALFALFA; PREDATION; NECTAR AB Sucrose was applied weekly throughout the growing season at three US locations (South Dakota [SD], Maryland [MD], and Kentucky [KY]), and coccinellids and aphids (Aphis glycines Matsumura [Hemiptera: Aphididae]) were sampled 24 h later. Total coccinellid densities were 50-77% greater in sugar-sprayed soybean than in untreated plots. Coccinella septempuncata L., Hippodamia convergens Gu,rin-M,neville, and Harmonia axyridis Pallas were more abundant where sugar was applied. Coleomegilla maculata (DeGeer) was found in equally low numbers in all treatments. Aphid densities were similar in both treatments, and only reached economically threatening levels in SD. Coccinellids digested sugar meals within 1 h of consumption (measured using the cold anthrone test). Despite this narrow window of detection, field-collected coccinellids frequently tested positive for fructose. Under natural conditions, sugar is commonly ingested by coccinellids and sugar sprays increase coccinellid densities and their consumption of sugar. Sugar sprays did not enhance biological control of aphids in this experiment. C1 [Seagraves, Michael P.; Lundgren, Jonathan G.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Kajita, Yukie; Obrycki, John J.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Weber, Donald C.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Lundgren, JG (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM jonathan.lundgren@ars.usda.gov NR 48 TC 8 Z9 10 U1 0 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 J9 BIOCONTROL JI Biocontrol PD JUN PY 2011 VL 56 IS 3 BP 305 EP 314 DI 10.1007/s10526-010-9337-3 PG 10 WC Entomology SC Entomology GA 768EI UT WOS:000290913900007 ER PT J AU Nirmala, X Olson, SR Holler, TC Cho, KH Handler, AM AF Nirmala, X. Olson, S. R. Holler, T. C. Cho, K. H. Handler, A. M. TI A DsRed fluorescent protein marker under polyubiquitin promoter regulation allows visual and amplified gene detection of transgenic Caribbean fruit flies in field traps SO BIOCONTROL LA English DT Article DE Sterile insect technique; Pest management; Tephritid fly traps; Tephritidae; Transgenic flies; Anastrepha suspensa ID CERATITIS-CAPITATA DIPTERA; INSECT TRANSGENESIS; SPERM MARKING; FLY DIPTERA; TEPHRITIDAE; TRANSFORMATION; SURVEILLANCE; PROSPECTS; IMPROVE; PEST AB Field population surveillance of a targeted insect pest species is critical in evaluating management programs such as the sterile insect technique. Fluorescent powder dyes currently used to distinguish released tephritids from the field population are not optimal in terms of reliability and human health issues. Genetically transformed tephritid species present the possibility of using fluorescent transgenes for marking. Here we studied the stability of DsRed fluorescence in transgenic flies maintained in aqueous torula yeast borax and propylene glycol. DsRed was stable in both solutions for three weeks by visual microscopic observations and could be used to unambiguously distinguish them from non-fluorescent wild type flies. To compensate for any potential ambiguity in visual identification a diagnostic PCR was developed that could specifically amplify the exotic heterologous marker gene. Therefore, the use of sterile transgenic insect strains carrying stably integrated fluorescent protein marker genes in biologically-based control programs could greatly improve released fly identification in pest management programs. C1 [Nirmala, X.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Nirmala, X.; Handler, A. M.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Olson, S. R.; Holler, T. C.; Cho, K. H.] USDA, APHIS PPQ, Gainesville, FL 32608 USA. RP Nirmala, X (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM xaviern@ufl.edu FU USDA-NIFA-Agriculture and Food Research Initiative FX The authors thank Drs. John Sivinski and Kenneth Bloem for critical comments on the manuscript and Jennifer Mestas and Alice Miano for technical assistance. Funding from the USDA-NIFA-Agriculture and Food Research Initiative is gratefully acknowledged. NR 31 TC 5 Z9 5 U1 0 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 J9 BIOCONTROL JI Biocontrol PD JUN PY 2011 VL 56 IS 3 BP 333 EP 340 DI 10.1007/s10526-011-9347-9 PG 8 WC Entomology SC Entomology GA 768EI UT WOS:000290913900010 ER PT J AU Nichols, NN Szynkarek, MP Skory, CD Gorsich, SW Lopez, MJ Guisado, GM Nichols, WA AF Nichols, Nancy N. Szynkarek, Matthew P. Skory, Christopher D. Gorsich, Steven W. Lopez, Maria J. Guisado, Gema M. Nichols, Wade A. TI Transformation and electrophoretic karyotyping of Coniochaeta ligniaria NRRL30616 SO CURRENT GENETICS LA English DT Article DE Fungal transformation; Protoplasts; Chromosomes; Mutagenesis; Electrophoretic karyotype; Fermentation inhibitors ID NITRATE REDUCTASE; HYGROMYCIN-B; GENE; LECYTHOPHORA; DETOXIFICATION; FERMENTATION; SYSTEM; FUNGI AB Coniochaeta ligniaria NRRL30616 is an ascomycete that grows with yeast-like appearance in liquid culture. The strain has potential utility for conversion of fibrous biomass to fuels or chemicals. Furans and other inhibitory compounds in lignocellulosic biomass are metabolized by NRRL30616, facilitating subsequent microbial fermentation of biomass sugars. This study undertook initial characterization of the genetic system of C. ligniaria NRRL30616. Transformation using hygromycin as a dominant selectable marker was achieved using protoplasts generated by incubating cells in 1% (v/v) beta-mercaptoethanol, followed by cell wall-digesting enzymes. Thirteen chromosomes with an estimated total size of 30.1 Mb were detected in C. ligniaria. The GC content of chromosomal DNA and of coding regions from cDNA sequences were 49.2 and 51.9%, respectively. This study is the first report of genome size, electrophoretic karyotype, and transformation system for a member of the Coniochaetales. C1 [Nichols, Nancy N.; Skory, Christopher D.] USDA, ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Szynkarek, Matthew P.; Nichols, Wade A.] Illinois State Univ, Sch Biol Sci, Normal, IL 61761 USA. [Gorsich, Steven W.] Cent Michigan Univ, Dept Biol, Mt Pleasant, MI 48859 USA. [Lopez, Maria J.; Guisado, Gema M.] Univ Almeria, Dept Biol Aplicada, Almeria, Spain. RP Nichols, NN (reprint author), USDA, ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM nancy.nichols@ars.usda.gov OI Lopez, Maria J./0000-0002-3153-3227 NR 26 TC 3 Z9 3 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0172-8083 J9 CURR GENET JI Curr. Genet. PD JUN PY 2011 VL 57 IS 3 BP 169 EP 175 DI 10.1007/s00294-010-0332-0 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA 766GZ UT WOS:000290770300002 PM 21222124 ER PT J AU Larson, TM Kendra, DF Busman, M Brown, DW AF Larson, Troy M. Kendra, David F. Busman, Mark Brown, Daren W. TI Fusarium verticillioides chitin synthases CHS5 and CHS7 are required for normal growth and pathogenicity SO CURRENT GENETICS LA English DT Article DE Chitin synthase; CHS; Fusarium spp.; Cell wall; Endophyte; Fumonisin ID MOTOR-LIKE DOMAIN; SACCHAROMYCES-CEREVISIAE; CLASS-V; ASPERGILLUS-NIDULANS; CLASS-VII; CLASS-I; GENE; MAIZE; MYOSIN; TRANSFORMATION AB Fusarium verticillioides is both an endophyte and a pathogen of maize and is a health threat in many areas of the world because it can contaminate maize with fumonisins, a toxic secondary metabolite. We identified eight putative chitin synthase (CHS) genes in F. verticillioides genomic sequence, and phylogenetic evidence shows that they group into seven established CHS gene classes. We targeted two CHSs (CHS5 and CHS7) for deletion analysis and found that both are required for normal hyphal growth and maximal disease of maize seedlings and ears. CHS5 and CHS7 encode a putative class V and class VII fungal chitin synthase, respectively; they are located adjacent to each other and are divergently transcribed. Fluorescent microscopy found that both CHS deficient strains produce balloon-shaped hyphae, while growth assays indicated that they were more sensitive to cell wall stressing compounds (e.g., the antifungal compound Nikkomycin Z) than wild type. Pathogenicity assays on maize seedlings and ears indicated that both strains were significantly reduced in their ability to cause disease. Our results demonstrate that both CHS5 and CHS7 are necessary for proper hyphal growth and pathogenicity of F. verticillioides on maize. C1 [Larson, Troy M.; Kendra, David F.; Busman, Mark; Brown, Daren W.] USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP Larson, TM (reprint author), USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM troy.larson@ars.usda.gov NR 52 TC 25 Z9 27 U1 4 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0172-8083 EI 1432-0983 J9 CURR GENET JI Curr. Genet. PD JUN PY 2011 VL 57 IS 3 BP 177 EP 189 DI 10.1007/s00294-011-0334-6 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 766GZ UT WOS:000290770300003 PM 21246198 ER PT J AU Coudron, TA Shelby, KS Ellersieck, MR Winston, BR Popham, HJR AF Coudron, Thomas A. Shelby, Kent S. Ellersieck, Mark R. Winston, Brittany R. Popham, Holly J. R. TI Developmental response of the beneficial predator Podisus maculiventris to change in dietary ascorbic acid concentration SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE predatory; entomophagous; biofortification; diet; development; odds ratio; biological control; Heteroptera; Pentatomidae; stink bug; vitamin C; spined soldier bug ID VITAMIN-C CONTENT; HELIOTHIS-VIRESCENS; ARTIFICIAL DIET; SAY HETEROPTERA; PENTATOMIDAE; PLANTS; SUSCEPTIBILITY; INSECTS; COST AB We report here the effects of a range of ascorbic acid concentrations (0.07, 0.3, 3.0, and 30.0 g l-1) in artificial diets on growth rates, adult weights, fecundity, and survival over two generations of the predatory stink bug, Podisus maculiventris (Say) (Heteroptera: Pentatomidae). Overall, a dietary level of 3.0 g l-1 gave the shortest developmental times over two generations. The likelihood of egg hatch at one ascorbic acid concentration compared to another concentration suggested that egg hatch increased as the concentration of ascorbic acid increased from 0.07 to 3.0 g l-1 and then declined from 3.0 to 30.0 g l-1. The combination of the maximum egg oviposition at 0.3 and 3.0 g l-1, egg hatch at 3.0 g l-1, and survival at 0.07 and 0.3 g l-1 suggests an overall superior performance at a concentration between 0.3 and 3.0 g l-1. Depletion of ascorbic acid below 3.0 g l-1 or addition of ascorbic acid above 3.0 g l-1 lowered the likelihood of egg hatch, which became more pronounced in the second generation. This is consistent with previously published information for phytophagous insects. C1 [Coudron, Thomas A.; Shelby, Kent S.; Popham, Holly J. R.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. [Ellersieck, Mark R.] Univ Missouri, Agr Expt Stn Statisticians, Columbia, MO 65211 USA. [Winston, Brittany R.] Lincoln Univ, Dept Life & Phys Sci, Jefferson City, MO 65101 USA. RP Coudron, TA (reprint author), ARS, Biol Control Insects Res Lab, USDA, 1503 S Providence Rd, Columbia, MO 65203 USA. EM tom.coudron@ars.usda.gov RI Shelby, Kent/E-1605-2011 OI Shelby, Kent/0000-0001-9859-3497 NR 18 TC 1 Z9 1 U1 1 U2 11 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUN PY 2011 VL 139 IS 3 BP 235 EP 241 DI 10.1111/j.1570-7458.2011.01127.x PG 7 WC Entomology SC Entomology GA 765ES UT WOS:000290686800006 ER PT J AU Woodall, CW Amacher, MC Bechtold, WA Coulston, JW Jovan, S Perry, CH Randolph, KC Schulz, BK Smith, GC Tkacz, B Will-Wolf, S AF Woodall, Christopher William Amacher, Michael C. Bechtold, William A. Coulston, John W. Jovan, Sarah Perry, Charles H. Randolph, KaDonna C. Schulz, Beth K. Smith, Gretchen C. Tkacz, Borys Will-Wolf, Susan TI Status and future of the forest health indicators program of the USA SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Criteria and indicators; Forest health; Forest inventory; Forest health monitoring; Forest health indicators ID UNITED-STATES; AMBIENT OZONE; CARBON STOCKS; GRADIENTS; POLLUTION; BUDGET AB For two decades, the US Department of Agriculture, Forest Service, has been charged with implementing a nationwide field-based forest health monitoring effort. Given its extensive nature, the monitoring program has been gradually implemented across forest health indicators and inventoried states. Currently, the Forest Service's Forest Inventory and Analysis program has initiated forest health inventories in all states, and most forest health indicators are being documented in terms of sampling protocols, data management structures, and estimation procedures. Field data from most sample years and indicators are available on-line with numerous analytical examples published both internally and externally. This investment in national forest health monitoring has begun to yield dividends by allowing evaluation of state/regional forest health issues (e.g., pollution and invasive pests) and contributing substantially to national/international reporting efforts (e.g., National Report on Sustainability and US EPA Annual Greenhouse Gas Estimates). With the emerging threat of climate change, full national implementation and remeasurement of a forest health inventory should allow for more robust assessment of forest communities that are undergoing unprecedented changes, aiding future land management and policy decisions. C1 [Woodall, Christopher William; Perry, Charles H.] US Forest Serv, No Res Stn, USDA, St Paul, MN USA. [Amacher, Michael C.] US Forest Serv, Rocky Mt Res Stn, USDA, Logan, UT USA. [Bechtold, William A.] US Forest Serv, So Res Stn, USDA, Asheville, NC USA. [Coulston, John W.; Randolph, KaDonna C.] US Forest Serv, So Res Stn, USDA, Knoxville, TN USA. [Jovan, Sarah] US Forest Serv, Pacific NW Res Stn, USDA, Portland, OR 97208 USA. [Schulz, Beth K.] US Forest Serv, Pacific NW Res Stn, USDA, Anchorage, AK USA. [Smith, Gretchen C.] Univ Massachusetts, Amherst, MA 01003 USA. [Tkacz, Borys] US Forest Serv, USDA, Washington, DC 20250 USA. [Will-Wolf, Susan] Univ Wisconsin, Madison, WI USA. RP Woodall, CW (reprint author), US Forest Serv, No Res Stn, USDA, St Paul, MN USA. EM cwoodall@fs.fed.us OI Woodall, Christopher/0000-0001-8076-6214; Perry, Charles/0000-0001-9377-5996 NR 77 TC 26 Z9 29 U1 6 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD JUN PY 2011 VL 177 IS 1-4 BP 419 EP 436 DI 10.1007/s10661-010-1644-8 PG 18 WC Environmental Sciences SC Environmental Sciences & Ecology GA 765TU UT WOS:000290732300031 PM 20711860 ER PT J AU Stephensen, CB Chantry, C Liang, L Styne, DM AF Stephensen, Charles B. Chantry, Caroline Liang, Lisa Styne, Dennis M. TI Reply to correspondence letter by S. A-zsoylu regarding our manuscript "Prevalence and risk factors for vitamin d deficiency among healthy infants and young children in Sacramento, California SO EUROPEAN JOURNAL OF PEDIATRICS LA English DT Letter C1 [Stephensen, Charles B.] Univ Calif Davis, Western Human Nutr Res Ctr, USDA, Immun & Dis Prevent Management Unit, Davis, CA 95616 USA. [Chantry, Caroline; Styne, Dennis M.] Univ Calif Davis, Sch Med, Dept Pediat, Davis, CA 95616 USA. [Liang, Lisa] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Liang, Lisa] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Stephensen, CB (reprint author), Univ Calif Davis, Western Human Nutr Res Ctr, USDA, Immun & Dis Prevent Management Unit, Davis, CA 95616 USA. EM charles.stephensen@ars.usda.gov NR 2 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0340-6199 J9 EUR J PEDIATR JI Eur. J. Pediatr. PD JUN PY 2011 VL 170 IS 6 BP 817 EP 818 DI 10.1007/s00431-011-1413-x PG 2 WC Pediatrics SC Pediatrics GA 766TA UT WOS:000290808200022 ER PT J AU Chang, S Condon, B Graves, E Uchimiya, M Fortier, C Easson, M Wakelyn, P AF Chang, SeChin Condon, Brian Graves, Elena Uchimiya, Minori Fortier, Chanel Easson, Michael Wakelyn, Phillip TI Flame Retardant Properties of Triazine Phosphonates Derivative with Cotton Fabric SO FIBERS AND POLYMERS LA English DT Article DE Flame retardant; Cotton; Flammability; Thermal degradation; Phosphorus ID NITROGEN; RESINS AB The flame retardant behavior of cotton fabric treated with phosphorus-nitrogen containing triazine compound was evaluated. It was found that cyanuric chloride (2,4,6-trichloro-1,3,5-triazine) is an excellent starting material for the preparation of phosphonate flame retardants that interact well with cotton to improve flame resistance (FR) performance. Tetraethyl 6-chloro-1,3,5-triazine-2,4-diyldiphosphonate (TECTDP) has been prepared by a simple one-step reaction in high yield (98.0 %). Cotton fabrics treated with TECTDP result in covalent bond formation between TECTDP and hydroxyl groups in cotton fabrics. This FR system provides an efficient flame retardant for cotton textiles at low cost to meet government mandates. In this study, the FR material was synthesized and characterized by (1)H and (13)C nuclear magnetic resonance (NMR), and LC-MS spectroscopy. The chemical structure of cotton twill fabric treated with TECTDP by pad-dry-cure method was analyzed by FT-MIR and SEM. Furthermore, the thermal and flammability properties were evaluated by thermogravimetric analysis (TGA), limiting oxygen index (LOI, ASTM D-2863-09), and a vertical flame test (ASTM D-6413-08). C1 [Chang, SeChin; Condon, Brian; Graves, Elena; Uchimiya, Minori; Fortier, Chanel; Easson, Michael] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Wakelyn, Phillip] Wakelyn Associates LLC, Washington, DC 20036 USA. RP Chang, S (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sechin.chang@ars.usda.gov FU USDA-ARS CRIS FX The authors wish to acknowledge the USDA-ARS CRIS for financial support. We also appreciate the skillful experimental assistance with Dr. Ryan Slopek and Christa Madison. NR 16 TC 20 Z9 21 U1 7 U2 37 PU KOREAN FIBER SOC PI SEOUL PA KOREA SCIENCE TECHNOLOGY CTR #501 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREA SN 1229-9197 J9 FIBER POLYM JI Fiber. Polym. PD JUN PY 2011 VL 12 IS 3 BP 334 EP 339 DI 10.1007/s12221-011-0334-7 PG 6 WC Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA 765WG UT WOS:000290739500007 ER PT J AU Ewald, SJ Kapczynski, DR Livant, EJ Suarez, DL Ralph, J McLeod, S Miller, C AF Ewald, Sandra J. Kapczynski, Darrell R. Livant, Emily J. Suarez, David L. Ralph, John McLeod, Scott Miller, Carolyn TI Association of Mx1 Asn631 variant alleles with reductions in morbidity, early mortality, viral shedding, and cytokine responses in chickens infected with a highly pathogenic avian influenza virus SO IMMUNOGENETICS LA English DT Article DE Chicken; Mx1; Influenza; Genetic resistance; Cytokines ID A VIRUS; ANTIVIRAL ACTIVITY; RESISTANCE GENE; MICE; INTERFERON; PROTEIN; REPLICATION; EXPRESSION; POLYMORPHISMS; POSITION-631 AB Myxovirus-resistance (Mx) proteins are produced by host cells in response to type I interferons, and some members of the Mx gene family in mammals have been shown to limit replication of influenza and other viruses. According to an early report, chicken Mx1 variants encoding Asn at position 631 have antiviral activity, whereas variants with Ser at 631 lack activity in experiments evaluating Mx1 complementary DNA (cDNA) expressed ectopically in a cell line. We evaluated whether the Mx1 631 dimorphism influenced pathogenesis of highly pathogenic avian influenza virus (HPAIV) infection in chickens of two commercial broiler lines, each segregating for Asn631 and Ser631 variants. Following intranasal infection with HPAIV strain A/Chicken/Queretaro/14588-19/1995 H5N2, chickens homozygous for Asn631 allele were significantly more resistant to disease based on early mortality, morbidity, or virus shedding than Ser631 homozygotes. Higher amounts of splenic cytokine transcripts were observed in the Ser631 birds after infection, consistent with higher viral loads seen in this group and perhaps contributing to their higher morbidity. Nucleotide sequence determination of Mx1 cDNAs demonstrated that the Asn631 variants in the two chicken lines differed at several amino acid positions outside 631. In vitro experiments with a different influenza strain (low pathogenicity) failed to demonstrate an effect of Mx1 Asn631 on viral replication suggesting that in vivo responses may differ markedly from in vitro, or that choice of virus strain may be critical in demonstrating effects of chicken Mx1. Overall, these studies provide the first evidence that Mx1 has antiviral effects in chickens infected with influenza virus. C1 [Ewald, Sandra J.] Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA. [Ewald, Sandra J.; Livant, Emily J.] Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA. [Kapczynski, Darrell R.; Suarez, David L.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. [Ralph, John; McLeod, Scott; Miller, Carolyn] Aviagen, Crossville, TN USA. RP Ewald, SJ (reprint author), Auburn Univ, Dept Pathobiol, 166 Greene Hall, Auburn, AL 36849 USA. EM ewaldsj@auburn.edu RI Kapczynski, Marcin/B-4696-2010 OI Kapczynski, Marcin/0000-0002-9220-0936 FU USDA National Institute of Food and Agriculture [2006-35205-16676]; USDA Agricultural Research Service [6612-32000-053] FX This project was supported by the National Research Initiative Competitive Grant no. 2006-35205-16676 from the USDA National Institute of Food and Agriculture, and USDA Agricultural Research Service CRIS project no. 6612-32000-053. We thank Clayton Floyd and Roger Brock for animal care. We also thank Dr. Igal Pevzner and Mr. Randy Shumate of Cobb-Vantress, Inc., for the chickens, and Dr. Peter Staeheli (University of Freiburg, Freiburg, Germany) for the expression construct for recombinant chicken IFN-alpha. We gratefully acknowledge the technical expertise of Ms. Milla Kaltenboeck, Ms. Tracy Faulkner, Mr. CamGreene, and Ms. Aniko Zsak. NR 38 TC 16 Z9 16 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PD JUN PY 2011 VL 63 IS 6 BP 363 EP 375 DI 10.1007/s00251-010-0509-1 PG 13 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA 763FR UT WOS:000290541200004 PM 21286706 ER PT J AU Li, B Wang, GS Chen, KF Vahey, DW Zhu, JY AF Li, Bo Wang, Gaosheng Chen, Kefu Vahey, David W. Zhu, J. Y. TI On Quantification of Residual Ink Content and Deinking Efficiency in Recycling of Mixed Office Waste Paper SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article AB Although (flotation) deinking has been a common industry practice for several decades, true residual ink content and deinking efficiency have never been quantified. Paper brightness and ERIC (Effective Residual Ink Concentration), based on measurements of the absorption coefficient of deinked pulp, have been used to determine performance of flotation deinking processes and the quality of deinked pulps. This study demonstrated a new method for quantifying true residual ink content of mixed office waste paper by determining the trace iron content in pulp samples. Two sets of laboratory studies were conducted, one using mixed pulp samples of different ink contents and the other using samples from flotation deinking experiments with different surfactant dosages. All pulp samples including flotation feeds and accepts were characterized by ERIC, brightness, and iron-content-derived ink content. The results suggest that ERIC and brightness are less suitable than iron content for evaluating the residual ink in very clean papers. In flotation studies, both ERIC and brightness predict lower deinking efficiencies than are obtained from iron-content measurements. However, brightness is suitable for evaluating the final quality of very clean deinked pulp because of its high accuracy at high brightness levels. C1 [Li, Bo; Chen, Kefu] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510641, Guangdong, Peoples R China. [Wang, Gaosheng] Tianjin Univ Sci & Technol, Tianjin Key Lab Pulp & Paper, Tianjin 304573, Peoples R China. [Vahey, David W.; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. RP Li, B (reprint author), S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510641, Guangdong, Peoples R China. EM ppboli@scut.edu.cn; jzhu@fs.fed.us FU Chinese Scholarship Council; State Key Laboratory of Pulp and Paper Engineering of China FX This work was conducted at the USDA Forest Service, Forest Products Laboratory (FPL) while Li was a visiting Ph.D. student at the University of Wisconsin-Madison and FPL and Wang was a visiting scientist at FPL and on official U.S. government time by Zhu and Vahey. We acknowledge the financial support from the Chinese Scholarship Council for Li's visiting appointment at FPL and the Young Investigator Fund of State Key Laboratory of Pulp and Paper Engineering of China. The authors would like to acknowledge Daniel Foster of FPL for conducting iron content analysis of pulp samples using ICP and Buckman Laboratory for providing surfactant. The work is in the public domain in the U.S. NR 12 TC 5 Z9 5 U1 3 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JUN 1 PY 2011 VL 50 IS 11 BP 6965 EP 6971 DI 10.1021/ie200148c PG 7 WC Engineering, Chemical SC Engineering GA 767GS UT WOS:000290843900048 ER PT J AU Hastrup, ACS Howell, C Jensen, B Green, F AF Hastrup, Anne Christine Steenkjaer Howell, Caitlin Jensen, Bo Green, Frederick, III TI Non-enzymatic depolymerization of cotton by fungal mimicking metabolites SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Hydrogen peroxide; Oxalic acid; Depolymerization; Fenton reaction; Cotton cellulose; EDTA ID BROWN-ROT FUNGI; DRIVEN FENTON REACTION; MOLECULAR-WEIGHT CHELATORS; OXALIC-ACID; WOOD DECAY; GLOEOPHYLLUM-TRABEUM; TYROMYCES-PALUSTRIS; CONIOPHORA-PUTEANA; HYDROXYL RADICALS; HYDROGEN-PEROXIDE AB Small, low molecular weight, non-enzymatic compounds have been linked to the early stages of brown rot decay as the enzymes involved with holocellulose degradation are too large to penetrate the S3 layer of intact wood cells. We investigated the most notable of these compounds, i.e. hydrogen peroxide, iron, and oxalic acid. The former two are involved in the Fenton reaction in which they react to form hydroxyl radicals, which cause an accelerated depolymerization in cotton cellulose. We found the same reaction to be caused by both iron Fe3+ and Fe2+. A 10 mM oxalic acid solution showed significant depolymerization effect on cotton cellulose. An oxalic acid/sodium oxalate buffered pH gradient had an inhibitory effect on the reduction of cellulose polymers at increased pH values. The organic iron chelator, EDTA, was found to promote depolymerization of cellulose in combination with Fenton's reagents, but inhibited the effect of oxalic acid in the absence of iron and hydrogen peroxide. Manganese was tested to see if metals other than iron could generate a significant impact on the degree of polymerization (DP) in cotton cellulose. Depolymerizing properties comparable to iron were seen. The results confirm that low molecular weight metabolites are capable of depolymerizing cellulose and suggest an importance of these mechanisms during incipient decay by brown rot fungi. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Hastrup, Anne Christine Steenkjaer; Green, Frederick, III] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Hastrup, Anne Christine Steenkjaer; Jensen, Bo] Univ Copenhagen, Inst Biol, Dept Biol, Microbiol Sect, DK-1307 Copenhagen K, Denmark. [Howell, Caitlin] Univ Maine, Sch Biol & Ecol, Orono, ME 04469 USA. RP Hastrup, ACS (reprint author), Solvgade 83H, DK-1307 Copenhagen K, Denmark. EM annech@bio.ku.dk RI Jensen, Bo/N-5082-2014; Howell, Caitlin/E-4274-2016 OI Jensen, Bo/0000-0002-0414-8999; Howell, Caitlin/0000-0002-9345-6642 NR 58 TC 10 Z9 10 U1 0 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD JUN PY 2011 VL 65 IS 3 BP 553 EP 559 DI 10.1016/j.ibiod.2011.02.006 PG 7 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA 765GR UT WOS:000290693900025 ER PT J AU MacNeil, MD Vukasinovic, N AF MacNeil, M. D. Vukasinovic, N. TI A prototype national cattle evaluation for sustained reproductive success in Hereford cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; genetic evaluation; reproduction; survival analysis ID SURVIVAL ANALYSIS; CENSORED RECORDS; PRODUCTIVE LIFE; BEEF FEMALES; DAIRY-COWS; LONGEVITY; MODELS; HERITABILITY; FERTILITY; SELECTION AB The objective of this research was to develop a prototype system for national cattle evaluation that would facilitate selection for improved fertility of daughters from Hereford sires. Raw data for this analysis were the birth dates of calves as reported by breeders to the American Hereford Association. Records from females entered this analysis with the reporting of a birth date for their first calf. At that time, females were required to be in contemporary groups of at least 3 animals and to have at least 2 additional paternal half-sibs also represented in the data. To explicitly define "sustained reproductive success," the philosophy taken was that a female that maintained a calving interval of 425 d or less would be considered successful. Females failing to meet this criterion were considered to be at the end of their successful lifetime. Data were analyzed using methodology for survival analysis with grouped data. Fixed contemporary groups were modeled as being time dependent, reflecting the females exposed for breeding in the same herd-year-season. Sire effects were time independent and considered random. Also included in the analysis were time-independent covariates for maternal weaning weight and total maternal calving ease from the national cattle evaluation of the American Hereford Association. Records from females still successfully in production at the time of this analysis, those that were transferred, those with calving intervals less than 280 d, and those that were successful until becoming donor dams for embryo transfer were considered censored. A total of 36,866 females contributed to this analysis, with 14,143 of these having censored records. The median number of females in a contemporary group was 6. A total of 3,323 sires had daughters with records. The median number of daughters per sire was 7. Heritability of sustained reproductive success on the underlying scale estimated from these data was approximately 0.05. Additional data accumulated over time will improve this genetic evaluation. Sustained reproductive success is important to the commercial beef industry, and results from this evaluation are expected to enhance the assessment of economic value of Hereford seedstock. C1 [MacNeil, M. D.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Vukasinovic, N.] Newsham Choice Genet, Chesterfield, MO 63005 USA. RP MacNeil, MD (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM mike.macneil@ars.usda.gov NR 30 TC 2 Z9 2 U1 1 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1712 EP 1718 DI 10.2527/jas.2010-3353 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400005 PM 21278118 ER PT J AU Snelling, WM Allan, MF Keele, JW Kuehn, LA Thallman, RM Bennett, GL Ferrell, CL Jenkins, TG Freetly, HC Nielsen, MK Rolfe, KM AF Snelling, W. M. Allan, M. F. Keele, J. W. Kuehn, L. A. Thallman, R. M. Bennett, G. L. Ferrell, C. L. Jenkins, T. G. Freetly, H. C. Nielsen, M. K. Rolfe, K. M. TI Partial-genome evaluation of postweaning feed intake and efficiency of crossbred beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; feed efficiency; genetic parameter ID QUANTITATIVE TRAIT LOCI; WIDE ASSOCIATION; GENETIC EVALUATION; ANGUS CATTLE; LINKAGE DISEQUILIBRIUM; BREEDING VALUES; FULL PEDIGREE; ADULT HEIGHT; GROWTH; INFORMATION AB The effects of individual SNP and the variation explained by sets of SNP associated with DMI, metabolic midtest BW, BW gain, and feed efficiency, expressed as phenotypic and genetic residual feed intake, were estimated from BW and the individual feed intake of 1,159 steers on dry lot offered a 3.0 Mcal/kg ration for at least 119 d before slaughter. Parents of these F-1 x F-1 (F-1(2)) steers were AI-sired F1 progeny of Angus, Charolais, Gelbvieh, Hereford, Limousin, Red Angus, and Simmental bulls mated to US Meat Animal Research Center Angus, Hereford, and MARC III composite females. Steers were genotyped with the BovineSNP50 BeadChip assay (Illumina Inc., San Diego, CA). Effects of 44,163 SNP having minor allele frequencies >0.05 in the F-1(2) generation were estimated with a mixed model that included genotype, breed composition, heterosis, age of dam, and slaughter date contemporary groups as fixed effects, and a random additive genetic effect with recorded pedigree relation-ships among animals. Variance in this population attributable to sets of SNP was estimated with models that partitioned the additive genetic effect into a polygenic component attributable to pedigree relationships and a genotypic component attributable to genotypic relationships. The sets of SNP evaluated were the full set of 44,163 SNP and subsets containing 6 to 40,000 SNP selected according to association with phenotype. Ninety SNP were strongly associated (P < 0.0001) with at least 1 efficiency or component trait; these 90 accounted for 28 to 46% of the total additive genetic variance of each trait. Trait-specific sets containing 96 SNP having the strongest associations with each trait explained 50 to 87% of additive variance for that trait. Expected accuracy of steer breeding values predicted with pedigree and genotypic relationships exceeded the accuracy of their sires predicted without genotypic information, although gains in accuracy were not sufficient to encourage that performance testing be replaced by genotyping and genomic evaluations. C1 [Snelling, W. M.; Allan, M. F.; Keele, J. W.; Kuehn, L. A.; Thallman, R. M.; Bennett, G. L.; Ferrell, C. L.; Jenkins, T. G.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Nielsen, M. K.; Rolfe, K. M.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. RP Snelling, WM (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM warren.snelling@ars.usda.gov NR 46 TC 42 Z9 44 U1 1 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1731 EP 1741 DI 10.2527/jas.2010-3526 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400007 PM 21297062 ER PT J AU Kuehn, LA Keele, JW Bennett, GL McDaneld, TG Smith, TPL Snelling, WM Sonstegard, TS Thallman, RM AF Kuehn, L. A. Keele, J. W. Bennett, G. L. McDaneld, T. G. Smith, T. P. L. Snelling, W. M. Sonstegard, T. S. Thallman, R. M. TI Predicting breed composition using breed frequencies of 50,000 markers from the US Meat Animal Research Center 2,000 Bull Project SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE breed composition; cattle; single nucleotide polymorphism ID GENOME-WIDE ASSOCIATION; GENETIC-STRUCTURE; CATTLE; POPULATION; HETEROSIS; EVOLUTION; TRAITS AB Knowledge of breed composition can be useful in multiple aspects of cattle production, and can be critical for analyzing the results of whole genome-wide association studies currently being conducted around the world. We examine the feasibility and accuracy of using genotype data from the most prevalent bovine genome-wide association studies platform, the Illumina BovineSNP50 array (Illumina Inc., San Diego, CA), to estimate breed composition for individual breeds of cattle. First, allele frequencies (of Illumina-defined allele B) of SNP on the array for each of 16 beef cattle breeds were defined by genotyping a large set of more than 2,000 bulls selected in cooperation with the respective breed associations to be representative of their breed. With these breed-specific allele frequencies, the breed compositions of approximately 2,000 two-, three-, and four-way cross (of 8 breeds) cattle produced at the US Meat Animal Research Center were predicted by using a simple multiple regression technique or Mendel (http://www.genetics.ucla.edu/software/mendel) and their genotypes from the Illumina BovineSNP50 array, and were then compared with pedigree-based estimates of breed composition. The accuracy of marker-based breed composition estimates was 89% when using either estimation method for all breeds except Angus and Red Angus (averaged 79%), based on comparing estimates with pedigree-based average breed composition. Accuracy increased to approximately 88% when these 2 breeds were combined into an aggregate Angus group. Additionally, we used a subset of these markers, approximately 3,000 that populate the Illumina Bovine3K (Illumina Inc.), to see whether breed composition could be estimated with similar accuracy when using this reduced panel of SNP makers. When breed composition was estimated using only SNP in common with the Bovine 3K array, accuracy was slightly reduced to 83%. These results suggest that SNP data from these arrays could be used to estimate breed composition in most US beef cattle in situations where pedigree is not known (e. g., multiple-sire natural service matings, non-source-verified animals in feedlots or at slaughter). This approach can aid analyses that depend on knowledge of breed composition, including identification and adjustment of breed-based population stratification, when performing genome-wide association studies on populations with incomplete pedigrees. In addition, SNP-based breed composition estimates may facilitate fitting cow germplasm to the environment, managing cattle in the feedlot, and tracing disease cases back to the geographic region or farm of origin. C1 [Kuehn, L. A.; Keele, J. W.; Bennett, G. L.; McDaneld, T. G.; Smith, T. P. L.; Snelling, W. M.; Thallman, R. M.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Sonstegard, T. S.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. RP Kuehn, LA (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Larry.Kuehn@ars.usda.gov NR 18 TC 18 Z9 19 U1 0 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1742 EP 1750 DI 10.2527/jas.2010-3530 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400008 PM 21278116 ER PT J AU Lay, DC Kattesh, HG Cunnick, JE Daniels, MJ Kranendonk, G McMunn, KA Toscano, MJ Roberts, MP AF Lay, D. C., Jr. Kattesh, H. G. Cunnick, J. E. Daniels, M. J. Kranendonk, G. McMunn, K. A. Toscano, M. J. Roberts, M. P. TI Effect of prenatal stress on subsequent response to mixing stress and a lipopolysaccharide challenge in pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE corticosteroid-binding globulin; cortisol; immune; prenatal; stress; swine ID PITUITARY-ADRENAL AXIS; IMMUNE-RESPONSE; CORTISOL; SOWS; INTERLEUKIN-6; BEHAVIOR; ALTERS; GESTATION; BIRTH; YOUNG AB Sows subjected to prenatal stress have been found to produce offspring that have altered responses to stress. Our objective was to determine if exposing a sow to stress would alter the response of the offspring to lipopolysaccharide (LPS) at 2 mo of age or their response to mixing stress at 4 mo of age. Sow treatments consisted of intravenous injections of ACTH (1 IU/kg of BW), exposure to rough handling for a 10-min duration (rough), or no treatment (control) once per week from d 42 to 77 of gestation. At 2 mo of age, pigs from each treatment, 1 per litter (n = 21, 17, and 15 for the ACTH, rough, and control treatments, respectively), were challenged with 2 mu g of LPS/kg of BW or saline, or served as a noninjected control. Their behavioral response to a human approach test and salivary cortisol were measured. At 4 mo of age, 1 pig from each treatment (n = 14, 14, and 15 for the ACTH, rough, and control treatments, respectively) was taken from its home pen and placed in a pen of unfamiliar pigs. At this time, a punch biopsy wound (6 x 6 mm) was created to measure the ability of the pig to heal the wound. At this same time, each pig received a 1-mL intramuscular injection of 20% ovine red blood cells (oRBC), and then a second injection of oRBC at 21 d postmixing. Blood samples were collected 3 times per week for 2 wk and then once a week for 4 more weeks. Blood samples were analyzed for cortisol, porcine corticosteroid-binding globulin, antibody response to oRBC, and nitric oxide production by macrophages. Behavior was recorded during the first 5 d after mixing. All pigs in the LPS challenge responded with characteristic sickness behavior; however, pigs in the rough treatment showed less sickness behavior than those in the other 2 treatments (P < 0.05). Maternal stress treatment did not affect (P < 0.43) salivary cortisol. Pigs from all treatments responded similarly to mixing stress with regard to cortisol, porcine corticosteroid-binding globulin, antibody titers, nitric oxide production, and hematology measures, and all pigs experienced the same amount of aggression in response to mixing. Without altering peripheral measures of stress responsivity, prenatal stress enhanced the ability of pigs to cope with a simulated immune challenge, which could prove to be an adaptation to challenging environments. C1 [Lay, D. C., Jr.; McMunn, K. A.; Toscano, M. J.] USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47980 USA. [Kattesh, H. G.; Roberts, M. P.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. [Cunnick, J. E.] Iowa State Univ, Dept Anim Sci, Ames, IA 50014 USA. [Daniels, M. J.] Univ Florida, Dept Stat, Gainesville, FL 32611 USA. [Kranendonk, G.] Univ Utrecht, Dept Foetal & Neonatal Biol, NL-3508 Utrecht, Netherlands. RP Lay, DC (reprint author), USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47980 USA. EM Don.Lay@ars.usda.gov FU USDA-National Research Initiative, Washington, DC [2000-02001]; USDA-Livestock Behavior Research Unit (West Lafayette, IN) FX This work was supported in part by a USDA-National Research Initiative Competitive Grant (No. 2000-02001; Washington, DC) and the USDA-Livestock Behavior Research Unit (West Lafayette, IN). Mention of a trade name, proprietary product, or specified equipment does not constitute a guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that may be suitable. NR 30 TC 11 Z9 11 U1 0 U2 15 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1787 EP 1794 DI 10.2527/jas.2010-3612 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400013 PM 21606444 ER PT J AU Weedman, SM Rostagno, MH Patterson, JA Yoon, I Fitzner, G Eicher, SD AF Weedman, S. M. Rostagno, M. H. Patterson, J. A. Yoon, I. Fitzner, G. Eicher, S. D. TI Yeast culture supplement during nursing and transport affects immunity and intestinal microbial ecology of weanling pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE immune response; intestinal microbiota; pig; transport; weaning; yeast culture ID NEWLY WEANED PIGLETS; GROWTH-PERFORMANCE; BETA-GLUCAN; NUTRIENT DIGESTIBILITY; PERFORIN EXPRESSION; NATURAL-KILLER; ANIMAL PLASMA; CELLS; HEALTH; COLONIZATION AB The objectives of this study were to determine the influence of a Saccharomyces cerevisiae fermentation product on innate immunity and intestinal microbial ecology after weaning and transport stress. In a randomized complete block design, before weaning and in a split-plot analysis of a 2 x 2 factorial arrangement of yeast culture (YY) and transport (TT) after weaning, 3-d-old pigs (n = 108) were randomly assigned within litter (block) to either a control (NY, milk only) or yeast culture diet (YY; delivered in milk to provide 0.1 g of yeast culture product/kg of BW) from d 4 to 21. At weaning (d 21), randomly, one-half of the NY and YY pigs were assigned to a 6-h transport (NY-TT and YY-TT) before being moved to nursery housing, and the other one-half were moved directly to nursery housing (NY-NT and YY-NT, where NT is no transport). The yeast treatment was a 0.2% S. cerevisiae fermentation product and the control treatment was a 0.2% grain blank in feed for 2 wk. On d 1 before transport and on d 1, 4, 7, and 14 after transport, blood was collected for leukocyte assays, and mesenteric lymph node, jejunal, and ileal tissue, and jejunal, ileal, and cecal contents were collected for Toll-like receptor expression (TLR); enumeration of Escherichia coli, total coliforms, and lactobacilli; detection of Salmonella; and microbial analysis. After weaning, a yeast x transport interaction for ADG was seen (P _ 0.05). Transport affected (P _ 0.09) ADFI after weaning. Yeast treatment decreased hematocrit (P _ 0.04). A yeast x transport interaction was found for counts of white blood cells (P _ 0.01) and neutrophils (P _ 0.02) and for the neutrophil-tolymphocyte ratio (P _ 0.02). Monocyte counts revealed a transport (P _ 0.01) effect. Interactions of yeast x transport (P _ 0.001) and yeast x transport x day (P _ 0.09) for TLR2 and yeast x transport (P _ 0.08) for TLR4 expression in the mesenteric lymph node were detected. Day affected lactobacilli, total coliform, and E. coli counts. More pigs were positive for Salmonella on d 7 and 14 than on d 4, and more YY-TT pigs were positive (P _ 0.07) on d 4. The number of bands for microbial amplicons in the ileum was greater for pigs in the control treatment than in the yeast treatment on d 0, and this number tended to decrease (P _ 0.066) between d 1 and 14 for all pigs. Similarity coefficients for jejunal contents were greater (P _ 0.03) for pigs fed NY than for those fed YY, but pigs fed YY had greater similarity coefficients for ileal (P _ 0.001) and cecal (P _ 0.058) contents. The number of yeast x transport x day interactions demonstrates the complexity of the stress and dietary relationship. C1 [Rostagno, M. H.; Eicher, S. D.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Yoon, I.; Fitzner, G.] Diamond V Mills Inc, Cedar Rapids, IA 52407 USA. RP Eicher, SD (reprint author), Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM Susan.Eicher@ars.usda.gov FU Diamond V Mills Inc. (Cedar Rapids, IA) FX Funded in part by Diamond V Mills Inc. (Cedar Rapids, IA). NR 32 TC 5 Z9 6 U1 7 U2 18 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1908 EP 1921 DI 10.2527/jas.2009-2539 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400027 PM 21606447 ER PT J AU Klotz, JL Bush, LP Strickland, JR AF Klotz, J. L. Bush, L. P. Strickland, J. R. TI A vascular contractility bioassay using bovine right ruminal artery and vein SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE bovine; norepinephrine; ruminal artery and vein; serotonin ID INFECTED TALL FESCUE; BLOOD-FLOW; CATTLE; ALKALOIDS; RESPONSES; EXTRACTS; HEIFERS; TISSUES; HAY AB Endophyte-infected (Neotyphodium coenophialum) tall fescue (Lolium arundinaceum) produces ergot alkaloids that are associated with peripheral vasoconstriction in grazing animals, and ingestion of these alkaloids may affect splanchnic vasculature. Peripheral effects of ergot alkaloids have been well documented previously in cattle using a lateral saphenous vein bioassay. Because of significant differences in morphological and functional characteristics between vasculature supporting digestive and peripheral tissues, the bovine foregut vascular model required validation. Experiments were conducted, using dose-responses to norepinephrine and serotonin that were normalized to either 0.12 M KCl, or 0.1 mM norepinephrine or serotonin, to compare responses of vessels equilibrated at different tensions on the day of collection or the day after collection. Segments of a branch of right ruminal artery and vein were collected from the ventral coronary groove of healthy cattle of mixed breed, age, and sex (n = 20) at local abattoirs. Cross-sections of the artery and vein were suspended on luminal supports in a chamber of a multimyograph containing continuously oxygenated Krebs-Henseleit buffer (95% O(2)/5% CO(2), pH 7.4; 37 degrees C). Vessels were allowed to equilibrate at either 0.5 or 1.0 g of tension for 1.5 h before exposure to a reference compound. Increasing concentrations of each biogenic amine were administered in 15-min intervals after buffer replacement. Data were normalized as a percentage of the contractile response induced by the reference compound for each tension and day of analysis. The ruminal artery and vein were both more responsive to KCl as a reference compound (P < 0.05) than to norepinephrine or serotonin and did not differ between days when normalized with KCl. Ruminal arteries had greater contractile responses (P < 0.05) when tension was set to 1.0 g, compared with 0.5 g, during equilibration. The ruminal vein response had a more stable maintenance of baseline tension in vessels equilibrated at 0.5 g of resting tension. Development of this bioassay allows separation of the effects tall fescue alkaloids exert on both the right ruminal artery and vein as representative vessels that service tissues functioning in nutrient absorption. C1 [Klotz, J. L.; Strickland, J. R.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. [Bush, L. P.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. RP Klotz, JL (reprint author), Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. EM james.klotz@ars.usda.gov NR 18 TC 4 Z9 4 U1 0 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2011 VL 89 IS 6 BP 1944 EP 1951 DI 10.2527/jas.2010-3532 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 767KL UT WOS:000290855400031 PM 21297058 ER PT J AU Bestelmeyer, BT Goolsby, DP Archer, SR AF Bestelmeyer, Brandon T. Goolsby, Darroc P. Archer, Steven R. TI Spatial perspectives in state-and-transition models: a missing link to land management? SO JOURNAL OF APPLIED ECOLOGY LA English DT Review DE Chihuahuan Desert; contagion; Iceland; monitoring; patch dynamics; regime shift; southern Great Plains; spatial dependence; thresholds ID ALTERNATIVE STABLE STATES; CATASTROPHIC REGIME SHIFTS; SOIL-EROSION THRESHOLDS; SOUTHERN NEW-MEXICO; VEGETATION DYNAMICS; SONORAN DESERT; RESTORATION ECOLOGY; HISTORICAL ECOLOGY; AUSTRALIAN SAVANNA; POSITIVE FEEDBACKS AB P>1. State-and-transition models (STMs) synthesize and communicate knowledge about the alternative states of an ecosystem and causes of state transitions. Data supported narrative descriptions within STMs are used to select or justify management actions. State transitions are characteristically heterogeneous in space and time, but spatial heterogeneity is seldom described in STMs, thereby limiting their utility. 2. We conducted a review that indicates how spatially explicit data can be used to improve STMs. We first identified three spatial scales at which spatial patterns and processes are manifest: patches, sites and landscapes. We then identified three classes of spatial processes that govern heterogeneity in state transitions at each scale and that can be considered in empirical studies, STM narratives and management interpretations. 3. First, spatial variations in land-use driver history (e.g. grazing use) can explain differences in the occurrence of state transitions within land areas that are otherwise uniform. Secondly, spatial dependence in response to drivers imposed by variations in soils, landforms and climate can explain how the likelihood of state transition varies along relatively static environmental gradients. Thirdly, state transition processes can be contagious, under control of vegetation-environment feedbacks, such that the spatiotemporal evolution of state transitions is predictable. 4. We suggest a strategy for considering each of the three spatial processes in the development of STM narratives. We illustrate how spatial data can be employed for describing early warning indicators of state transition, identifying areas that are most susceptible to state transitions, and designing and implementing monitoring schemes. 5. Synthesis and applications. State-and-transition models are increasingly important tools for guiding land-management activities. However, failure to adequately represent spatial processes in STMs can limit their ability to identify the initiation, risk and causes of state transitions and, therefore, the appropriate management responses. We suggest that multi-scaled studies targeted to different kinds of ecosystems can be used to uncover evidence of spatial processes. Such evidence should be included in STM narratives and can lead to novel interpretations of land change and improved management. C1 [Bestelmeyer, Brandon T.] New Mexico State Univ, USDA, ARS, Jornada Expt Range & Jornada Basin LTER, Las Cruces, NM 88003 USA. [Goolsby, Darroc P.] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Archer, Steven R.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. RP Bestelmeyer, BT (reprint author), New Mexico State Univ, USDA, ARS, Jornada Expt Range & Jornada Basin LTER, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM bbestelm@nmsu.edu FU USDA-NRI; National Science Foundation [DEB 0080412] FX This work was supported by a grant from the USDA-NRI Biology of Weedy and Invasive Species programme and the National Science Foundation Long-Term Ecological Research programme (DEB 0080412). We thank Kris Havstad, Katie Predick, Rachel Standish and two anonymous referees for comments on earlier drafts of the paper and Caiti Steele and Judy Ward for assistance with the data. NR 85 TC 51 Z9 52 U1 6 U2 79 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-8901 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2011 VL 48 IS 3 BP 746 EP 757 DI 10.1111/j.1365-2664.2011.01982.x PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 763VF UT WOS:000290587100028 ER PT J AU Maiga, HA Bauer, ML Dahlen, CR Badaruddin, M Scholljegerdes, EJ AF Maiga, H. A. Bauer, M. L. Dahlen, C. R. Badaruddin, M. Scholljegerdes, E. J. TI Mustard bran in lactating dairy cow diets SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE oriental mustard bran; milk; organoleptic; lactating cow ID CONJUGATED LINOLEIC-ACID; COTTONSEED MEAL; CANOLA-MEAL; DRY-MATTER; MILK; DIGESTIBILITY; RATIONS; FAT AB Two trials using lactating Holstein cows were conducted to evaluate effects of a diet containing oriental mustard bran on dry matter intake (DMI), milk production, milk components, and organoleptic properties. In experiment 1, 34 lactating cows (24 multiparous and 10 primiparous; days in milk >= 50 d) were used in a switchback design to determine the lactational response and organoleptic quality of milk when the diet contained 8% oriental mustard bran (MB) versus h control diet (CON). Mustard bran replaced a portion of soybean meal and all the beet pulp in the CON diet. Milk yields were greater for cows fed the NIB diet; however, no differences were found in DMI, 3.5% fat(FCM) or solids-corrected milk. Milk components and components production were not affected by treatment. Milk organoleptic qualities were not affected by diet. In experiment 2, 22 lactating cows (16 multiparous and 6 primiparous; days in milk >= 21 d) were assigned randomly within parity to receive MB or CON from wk 4 to 19 postpartum in a randomized complete block design. Cows were fed CON wk 1 to 3 postpartum. The MB diet contained the same ingredients as the CON, except sunflower seed and a portion of soybean meal were replaced with mustard bran. Milk and components data were collected during wk 3 postpartum and used as covariates to adjust treatment means. Intake was greater for cows fed the NIB diet; however, daily milk, 3.5% FCM, and solids-corrected milk yields were not different between diets. Milk components and component yields were not affected by treatment. Milk urea concentration was less for cows fed the MB diet. Although cows fed the MB diet had greater DMI, this was not translated into a higher milk 3.5% FCM/DMI production efficiency ratio. During experiment 2, many cows fed MB experienced minor to severe hemolysis with bloody urine. This hemolysis believed to be caused by the S-methyl-cysteine sulfoxide contained in mustard bran could have affected milk production efficiency. The increased milk yield observed in experiment 1 was not observed in experiment 2. Adding 8% of MB to lactating cow diets had a mixed effect on DMI and milk production. Milk component yields and milk quality were not affected. Feeding this level of MB presents a hemolytic danger to lactating dairy cows. C1 [Maiga, H. A.] Univ Minnesota, Dept Agr, Div Anim Sci, Crookston, MN 56716 USA. [Bauer, M. L.; Dahlen, C. R.] N Dakota State Univ, Dept Anim Sci, Fargo, ND 58108 USA. [Badaruddin, M.] Minn Dak Growers Ltd, Grand Forks, ND 58208 USA. [Scholljegerdes, E. J.] USDA, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Maiga, HA (reprint author), Univ Minnesota, Dept Agr, Div Anim Sci, 2900 Univ Ave, Crookston, MN 56716 USA. EM hmaiga@umn.edu NR 29 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2011 VL 94 IS 6 BP 3054 EP 3062 DI 10.3168/jds.2010-3971 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 766JG UT WOS:000290777800040 PM 21605775 ER PT J AU Aguerre, MJ Wattiaux, MA Powell, JM Broderick, GA Arndt, C AF Aguerre, M. J. Wattiaux, M. A. Powell, J. M. Broderick, G. A. Arndt, C. TI Effect of forage-to-concentrate ratio in dairy cow diets on emission of methane, carbon dioxide, and ammonia, lactation performance, and manure excretion SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE methane; ammonia; forage; dairy cow ID VOLATILE FATTY-ACID; RUMEN FERMENTATION; MILK-PRODUCTION; UREA CONCENTRATION; HOLSTEIN HEIFERS; BEEF-CATTLE; CORN-SILAGE; FEED-INTAKE; NITROGEN; PROTEIN AB Holstein cows housed in a modified tie-stall barn were used to determine the effect of feeding diets with different forage-to-concentrate ratios (F:C) on performance and emission of CH(4), CO(2) and manure NH(3)-N. Eight multiparous cows (means +/- standard deviation): 620 +/- 68 kg of body weight; 52 +/- 34 d in milk and 8 primiparous cows (546 +/- 38 kg of body weight; 93 +/- 39 d in milk) were randomly assigned to 1 of 4 airflow controlled chambers, constructed to fit 4 cows each. Chambers were assigned to dietary treatment sequences in a single 4 x 4 Latin square design. Dietary treatments, fed as 16.2% crude protein total mixed rations included the following F:C ratio: 47:53, 54:46, 61:39, and 68:32 [diet dry matter (DM) basis]. Forage consisted of alfalfa silage and corn silage in a 1:1 ratio. Cow performance and emission data were measured on the last 7 d and the last 4 d, respectively of each 21-d period. Air samples entering and exiting each chamber were analyzed with a photo-acoustic field gas monitor. In a companion study, fermentation pattern was studied in 8 rumen-cannulated cows. Increasing F:C ratio in the diet had no effect on DM intake (21.1 +/- 1.5 kg/d), energy-corrected milk (ECM, 37.4 +/- 2.2 kg/d), ECM/DM intake (1.81 +/- 0.18), yield of milk fat, and manure excretion and composition; however, it increased milk fat content linearly by 7% and decreased linearly true protein, lactose; and solids-not-fat content (by 4, 1, and 2%, respectively) and yield (by 10, 6, and 6%, respectively), and milk N-to-N intake ratio. On average 93% of the N consumed by the cows in the chambers was accounted for as milk N, manure N, or emitted NH(3)-N. Increasing the F:C ratio also increased ruminal pH linearly and affected concentrations of butyrate and isovalerate quadratically. Increasing the F:C ratio from 47:53 to 68:32 increased CH(4) emission from 538 to 648 g/cow per day, but had no effect on manure NH(3)-N emission (14.1 +/- 3.9 g/cow per day) and CO2 emission (18,325 +/- 2,241 g/cow per day). In this trial, CH(4) emission remained constant per unit of neutral detergent fiber intake (1 g of CH(4) was emitted for every 10.3 g of neutral detergent fiber consumed by the cow), but increased from 14.4 to 18.0 g/kg of ECM when the percentage of forage in the diet increased from 47 to 68%. Although the pattern of emission within a day was distinct for each gas, emissions were higher between morning feeding (0930 h) and afternoon milking (1600 h) than later in the day. Altering the level of forage within a practical range and rebalancing dietary crude protein with common feeds of the Midwest of the United States had no effects on manure NH(3)-N emission but altered CH(4) emission. C1 [Aguerre, M. J.; Wattiaux, M. A.; Arndt, C.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Powell, J. M.; Broderick, G. A.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Wattiaux, MA (reprint author), Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. EM wattiaux@wisc.edu FU USDA-ARS [58-3655-7-627]; USDA [NE-0144, WIS01547] FX This research was supported in part by the USDA-ARS specific cooperative agreement # 58-3655-7-627 and by USDA-Hatch Multi-State Research Formula fund NE-0144 award number WIS01547. The authors thank Robbin Smith for her assistance in chamber maintenance, calibrations, and conduct of these experiments; Brian Holmes (Department of Biological Systems Engineering, University of Wisconsin-Madison, WI) for his assistance in chamber calibrations; Nancy Betzold and her barn crew for feeding and animal care at the US Dairy Forage Center Research Farm (Prairie du Sac, WI); Wendy Radloff and Mary Becker (US Dairy Forage Research Center, Madison, WI) for assisting with laboratory analyses; and Jacob Finer, Teresa Kaiser, and other UW-Madison students for helping during sample collection and processing. NR 62 TC 60 Z9 64 U1 4 U2 77 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2011 VL 94 IS 6 BP 3081 EP 3093 DI 10.3168/jds.2010-4011 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 766JG UT WOS:000290777800042 PM 21605777 ER PT J AU Wiggans, GR VanRaden, PM Cooper, TA AF Wiggans, G. R. VanRaden, P. M. Cooper, T. A. TI The genomic evaluation system in the United States: Past, present, future SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE genomic evaluation; SNP effect; reliability ID SINGLE-NUCLEOTIDE POLYMORPHISMS; PREDICTIONS; SELECTION; GENOTYPES; CATTLE AB Implementation of genomic evaluation has caused profound changes in dairy cattle breeding. All young bulls bought by major artificial insemination organizations now are selected based on such evaluation. Evaluation reliability can reach approximately 75% for yield traits, which is adequate for marketing semen of 2-yr-old bulls. Shortened generation interval from using genomic evaluations is the most important factor in increasing the rate of genetic improvement. Genomic evaluations are based on 42,503 single nucleotide polymorphisms (SNP) genotyped with technology that became available in 2007. The first unofficial USDA genomic evaluations were released in 2008 and became official for Holsteins, Jerseys, and Brown Swiss in 2009. Evaluation accuracy has increased steadily from including additional bulls with genotypes and traditional evaluations (predictor animals). Some of that increase occurs automatically as young genotyped bulls receive a progeny test evaluation at 5 yr of age. Cow contribution to evaluation accuracy is increased by decreasing mean and variance of their evaluations so that they are similar to bull evaluations. Integration of US and Canadian genotype databases was critical to achieving acceptable initial accuracy and continues to benefit both countries. Genotype exchange with other countries added predictor bulls for Brown Swiss. In 2010, a low-density chip with 2,900 SNP and a high-density chip with 777,962 SNP were released. The low-density chip has increased greatly the number of animals genotyped and is expected to replace microsatellites in parentage verification. The high-density chip can increase evaluation accuracy by better tracking of loci responsible for genetic differences. To integrate information from chips of various densities, a method to impute missing genotypes was developed based on splitting each genotype into its maternal and paternal haplotypes and tracing their inheritance through the pedigree. The same method is used to impute genotypes of nongenotyped dams based on genotyped progeny and mates. Reliability of resulting evaluations is discounted to reflect errors inherent in the process. Further increases in evaluation accuracy are expected because of added predictor animals and more SNP. The large population of existing genotypes can be used to evaluate new traits; however, phenotypic observations must be obtained for enough animals to allow estimation of SNP effects with sufficient accuracy for application to the general population. C1 [Wiggans, G. R.; VanRaden, P. M.; Cooper, T. A.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. RP Wiggans, GR (reprint author), ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. EM George.Wiggans@ars.usda.gov NR 39 TC 102 Z9 104 U1 2 U2 29 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2011 VL 94 IS 6 BP 3202 EP 3211 DI 10.3168/jds.2010-3866 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 766JG UT WOS:000290777800054 PM 21605789 ER PT J AU Allen, C Valles, SM Strong, CA AF Allen, Clare Valles, Steven M. Strong, Charles A. TI Multiple virus infections occur in individual polygyne and monogyne Solenopsis invicta ants SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Multiple infections; Genetic diversity; Multiplex PCR; Solenopsis invicta virus; Fire ant ID IMPORTED FIRE ANT; DEPENDENT RNA-POLYMERASE; HYMENOPTERA-FORMICIDAE; HONEYBEE COLONIES; GENETIC DIVERSITY; SOCIAL INSECTS; BEE VIRUSES; PARASITES; PCR; POPULATIONS AB Concurrent infections of Solenopsis invicta colonies with S. invicta virus 1 (SINV-1). SINV-2, and SINV-3 has been reported. However, whether individual ants were capable of supporting multiple virus infections simultaneously was not known, nor whether the social form of the colony (polygyne or monogyne) had an influence on the occurrence of multiple infection rates in individual ants. S. invicta field populations were sampled sequentially to establish whether multiple virus infections co-occurred in individual worker ants. In addition, the intra-colony virus infection rates were compared in monogyne and polygyne field colonies to determine whether social form played a role in the viral infection prevalence. All combinations of virus infection (SINV-1, SINV-2, or SINV-3 alone, SINV-1 & SINV-2, SINV-1 & SINV-3, SINV-2 & SINV-3, and SINV-1, SINV-2 & SINV-3) were detected in individual worker ants as well as queens in the field. Thus, individual S. invicta ants can be infected simultaneously with all combinations of the S. invicta viruses. Colony social form did have an influence on the intra-colony prevalence of multiple S. invicta virus infections. Polygyne colonies exhibited significantly greater intra- and inter-colony single and multiple virus infections compared with monogyne colonies. Published by Elsevier Inc. C1 [Allen, Clare; Valles, Steven M.; Strong, Charles A.] USDA ARS, Imported Fire Ant & Household Insects Res Unit, Gainesville, FL 32608 USA. RP Valles, SM (reprint author), USDA ARS, Imported Fire Ant & Household Insects Res Unit, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 43 TC 8 Z9 8 U1 1 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUN PY 2011 VL 107 IS 2 BP 107 EP 111 DI 10.1016/j.jip.2011.03.005 PG 5 WC Zoology SC Zoology GA 768GO UT WOS:000290922100003 PM 21439294 ER PT J AU Rowley, DL Popham, HJR Harrison, RL AF Rowley, Daniel L. Popham, Holly J. R. Harrison, Robert L. TI Genetic variation and virulence of nucleopolyhedroviruses isolated worldwide from the heliothine pests Helicoverpa armigera, Helicoverpa zea, and Heliothis virescens SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Heliothis virescens (Fabricius); Helicoverpa zea (Boddie); Helicoverpa armigera (Hubner); Tobacco budworm; Corn earworm; Nucleopolyhedrovirus; Baculovirus; Biological control ID NUCLEAR-POLYHEDROSIS-VIRUS; GENOMIC SEQUENCE-ANALYSIS; SINGLE NUCLEOCAPSID NUCLEOPOLYHEDROVIRUS; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS; POLYMERASE-CHAIN-REACTION; DNA PHOTOLYASE GENES; MULTIPLE-NUCLEOPOLYHEDROVIRUS; LEPIDOPTERA-NOCTUIDAE; MAMESTRA-CONFIGURATA; MOLECULAR-IDENTIFICATION AB To assess the diversity and relationships of baculoviruses found in insects of the heliothine pest complex, a PCR-based method was used to classify 90 samples of nucleopolyhedrovirus (NPV; Baculoviridae: Alphabaculovirus) obtained worldwide from larvae of Heliothis virescens, Helicoverpa zea, and Helicoverpa armigera. Partial nucleotide sequencing and phylogenetic analysis of three highly conserved genes (lef-8, lef-9, and polh) indicated that 67 of these samples contained isolates of the H. zea-H. armigera single nucleopolyhedrovirus (Hz/HaSNPV) species group. Eighteen of the samples contained isolates of a multiple NPV from H. armigera. HearMNPV, and five of the samples contained isolates of Autographa californica MNPV (AcMNPV). Sequencing and analysis of an additional seven loci (orf5/orf5b, hr3-orf62, orf26, orf79, orf124/orf117a, orf42, and a part of the region between hr2 and hr3) in the Hz/HearSNPV isolates further classified these viruses into two groups of HearSNPV variants mostly from India and China and a third group of HzSNPV variants. Some of the samples contained isolates of more than one virus. In bioassays of a selection of isolates against H. zea, the commercially available Gemstar (R) isolate of HzSNPV killed larvae faster than most other Hz/HaSNPV and HearMNPV isolates. Gemstar (R) and two HearMNPV isolates exhibited significantly higher LC(50)s than the Hz/HearSNPV isolates tested. This study expands significantly on what we know about the variation of heliothine NPV populations, provides novel information on the distinct groups in which these NPVs occur, and contributes to the knowledge required for improvement of heliothine baculoviruses as biological control agents. Published by Elsevier Inc. C1 [Harrison, Robert L.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, BARC W, Beltsville, MD 20705 USA. [Popham, Holly J. R.] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Harrison, RL (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, BARC W, Bldg 011A,Room 214,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Daniel.Rowley@ars.usda.gov; Holly.Popham@ar-s.usda.gov; Robert.L.Harrison@ars.usda.gov RI Harrison, Robert/I-4707-2014; OI Harrison, Robert/0000-0002-8348-3874 NR 66 TC 20 Z9 20 U1 0 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUN PY 2011 VL 107 IS 2 BP 112 EP 126 DI 10.1016/j.jip.2011.03.007 PG 15 WC Zoology SC Zoology GA 768GO UT WOS:000290922100004 PM 21439295 ER PT J AU Goff, B Moore, KJ Fales, SL Pedersen, JF AF Goff, Ben M. Moore, Kenneth J. Fales, Steven L. Pedersen, Jeffery F. TI Comparison of gas chromotography, spectrophotometry and near infrared spectroscopy to quantify prussic acid potential in forages SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE prussic acid; gas chromatography; spectrophotometry; near infrared spectroscopy; sorghum; sudangrass ID REFLECTANCE SPECTROSCOPY; SORGHUM-VULGARE; QUANTIFICATION; DHURRIN; PLANTS; SUDANGRASS; TISSUE AB BACKGROUND: Sorghum [Sorghum bicolor (L.) Moench] has been shown to contain the cyanogenic glycoside dhurrin, which is responsible for the disorder known as prussic acid poisoning in livestock. The current standard method for estimating hydrogen cyanide (HCN) uses spectrophotometry to measure the aglycone, p-hydroxybenzaldehyde (p-HB), after hydrolysis. Errors may occur due to the inability of this method to solely estimate the absorbance of p-HB at a given wavelength. The objective of this study was to compare the use of gas chromatography (GC) and near infrared spectroscopy (NIRS) methods, along with a spectrophotometry method to estimate the potential for prussic acid (HCNp) of sorghum and sudangrasses over three stages maturities. RESULTS: It was shown that the GC produced higher HCNp estimates than the spectrophotometer for the grain sorghums, but lower concentrations for the sudangrass. Based on what is known about the analytical process of each method, the GC data is likely closer to the true HCNp concentrations of the forages. Both the GC and spectrophotometry methods yielded robust equations with the NIRS method; however, using GC as the calibration method resulted in more accurate and repeatable estimates. CONCLUSION: The HCNp values obtained from using the GC quantification method are believed to be closer to the actual values of the forage, and that use of this method will provide a more accurate and easily automated means of quantifying prussic acid. (C) 2011 Society of Chemical Industry C1 [Goff, Ben M.; Moore, Kenneth J.; Fales, Steven L.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Pedersen, Jeffery F.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Goff, B (reprint author), Iowa State Univ, Dept Agron, 2101 Agron Hall, Ames, IA 50011 USA. EM ben.goff@uky.edu NR 26 TC 4 Z9 4 U1 3 U2 23 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD JUN PY 2011 VL 91 IS 8 BP 1523 EP 1526 DI 10.1002/jsfa.4366 PG 4 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 767MK UT WOS:000290860500024 PM 21541942 ER PT J AU Barrack, MT Van Loan, MD Rauh, MJ Nichols, JF AF Barrack, Michelle T. Van Loan, Marta D. Rauh, Mitchell J. Nichols, Jeanne F. TI Body Mass, Training, Menses, and Bone in Adolescent Runners: A 3-yr Follow-up SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article DE CROSS-COUNTRY; OSTEOPOROSIS; BONE DEVELOPMENT; ENERGY DEFICIT; AMENORRHEA ID MINERAL DENSITY; ANOREXIA-NERVOSA; LUMBAR SPINE; WEIGHT-GAIN; WOMEN; TURNOVER; LEPTIN; AGE; NORMALIZATION; OSTEOPOROSIS AB BARRACK, M. T., M. D. VAN LOAN, M. J. RAUH, and J. F. NICHOLS. Body Mass, Training, Menses, and Bone in Adolescent Runners: A 3-yr Follow-up. Med. Sci. Sports Exerc., Vol. 43, No. 6, pp. 959-966, 2011. Endurance runners with low bone mass during adolescence may risk attaining a low peak bone mineral density (BMD) in adulthood. Alternatively, they may mature late and undergo delayed bone mineral accumulation. Purpose: The purpose of this study was to evaluate 40 adolescent runners (aged 15.9 +/- 0.2 yr) at two time points, approximately 3 yr apart, to assess bone mass status and identify variables associated with bone mass change. Methods: Follow-up measures included a questionnaire to assess menstrual status, training, and sports participation history, height and weight, and a dual-energy x-ray absorptiometry scan to assess total body, total hip, and lumbar spine BMD, bone mineral content (BMC), BMD z-score, and body composition. We used -1 and -2 BMD z-score cutoffs to categorize runners with low bone mass. Results: Eighty-seven percent of girls with low BMD at baseline had low BMD at the follow-up. Girls with low compared with normal baseline BMD had lower follow-up adjusted total body (2220.4 +/- 65.8 vs 2793.1 +/- 68.2 g, P < 0.001), total hip (27.0 +/- 1 vs 33.9 +/- 1.0 g, P < 0.05), and lumbar spine (47.8 +/- 2.0 vs 66.3 +/- 2.2 g, P < 0.001) BMC values. Variables related to 3-yr training volume, menstrual function, age, developmental stage, and change in body mass explained 29%-54% of the variability in BMC change. Conclusions: The majority of adolescent runners with low BMD at baseline had low BMD after a 3-yr follow-up. Our observations suggest that "catch-up'' accrual may be difficult and, thus, emphasize the importance of gaining adequate bone mineral during the early adolescent years. C1 [Barrack, Michelle T.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Van Loan, Marta D.] Univ Calif Davis, ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Rauh, Mitchell J.] Rocky Mt Univ Hlth Profess, Grad Program Orthopaed & Sports Phys Therapy, Provo, UT USA. [Rauh, Mitchell J.; Nichols, Jeanne F.] San Diego State Univ, Sch Exercise & Nutr Sci, San Diego, CA 92182 USA. RP Barrack, MT (reprint author), Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. EM michellebarrack@gmail.com FU National Athletic Trainers Association; San Diego State University FX Funded by grants from the National Athletic Trainers Association and the San Diego State University Fred Kasch Endowment. NR 30 TC 13 Z9 13 U1 3 U2 7 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD JUN PY 2011 VL 43 IS 6 BP 959 EP 966 DI 10.1249/MSS.0b013e318201d7bb PG 8 WC Sport Sciences SC Sport Sciences GA 764TW UT WOS:000290657300004 PM 20980925 ER PT J AU Li, XR Zhu, CS Lin, ZW Wu, Y Zhang, DB Bai, GH Song, WX Ma, JX Muehlbauer, GJ Scanlon, MJ Zhang, M Yu, JM AF Li, Xianran Zhu, Chengsong Lin, Zhongwei Wu, Yun Zhang, Dabao Bai, Guihua Song, Weixing Ma, Jianxin Muehlbauer, Gary J. Scanlon, Michael J. Zhang, Min Yu, Jianming TI Chromosome Size in Diploid Eukaryotic Species Centers on the Average Length with a Conserved Boundary SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE chromosome size; genome evolution; evolutionary modeling ID SEX-CHROMOSOMES; GENOME; EVOLUTION; MODEL; DISTRIBUTIONS; STABILITY; KARYOTYPE; SEQUENCE; POSITION; MITOSIS AB Understanding genome and chromosome evolution is important for understanding genetic inheritance and evolution. Universal events comprising DNA replication, transcription, repair, mobile genetic element transposition, chromosome rearrangements, mitosis, and meiosis underlie inheritance and variation of living organisms. Although the genome of a species as a whole is important, chromosomes are the basic units subjected to genetic events that coin evolution to a large extent. Now many complete genome sequences are available, we can address evolution and variation of individual chromosomes across species. For example, "How are the repeat and nonrepeat proportions of genetic codes distributed among different chromosomes in a multichromosome species?" "Is there a general rule behind the intuitive observation that chromosome lengths tend to be similar in a species, and if so, can we generalize any findings in chromosome content and size across different taxonomic groups?" Here, we show that chromosomes within a species do not show dramatic fluctuation in their content of mobile genetic elements as the proliferation of these elements increases from unicellular eukaryotes to vertebrates. Furthermore, we demonstrate that, notwithstanding the remarkable plasticity, there is an upper limit to chromosome-size variation in diploid eukaryotes with linear chromosomes. Strikingly, variation in chromosome size for 886 chromosomes in 68 eukaryotic genomes (including 22 human autosomes) can be viably captured by a single model, which predicts that the vast majority of the chromosomes in a species are expected to have a base pair length between 0.4035 and 1.8626 times the average chromosome length. This conserved boundary of chromosome-size variation, which prevails across a wide taxonomic range with few exceptions, indicates that cellular, molecular, and evolutionary mechanisms, possibly together, confine the chromosome lengths around a species-specific average chromosome length. C1 [Zhang, Dabao; Zhang, Min] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Li, Xianran; Zhu, Chengsong; Lin, Zhongwei; Wu, Yun; Bai, Guihua; Yu, Jianming] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS USA. [Song, Weixing] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [Ma, Jianxin] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, Minneapolis, MN 55455 USA. [Scanlon, Michael J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. RP Zhang, M (reprint author), Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. EM minzhang@purdue.edu; jyu@ksu.edu RI Zhu, Chengsong/F-8045-2011; Li, Xianran/I-5651-2012; OI Li, Xianran/0000-0002-4252-6911; Yu, Jianming/0000-0001-5326-3099 FU National Science Foundation [DBI-0820610, IIS-0844945]; National Institute of Health [NIH/NCI U01-CA128535-01]; Department of Defense [W81XWH-08-1-0065]; Purdue University; National Research Initiative of the US-DA-CSREES [2006-03578]; Kansas State University FX We are very grateful to Dr. Ingo Schubert and Dr. Brandon Gaut for their critical comments of the manuscript. This work is supported by the National Science Foundation (DBI-0820610; IIS-0844945), the National Institute of Health (NIH/NCI U01-CA128535-01), the Department of Defense (W81XWH-08-1-0065), the Purdue University Discovery Park Seed Grant, the National Research Initiative of the US-DA-CSREES (2006-03578), and the Targeted Excellence Program of Kansas State University. NR 36 TC 6 Z9 6 U1 1 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0737-4038 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD JUN PY 2011 VL 28 IS 6 BP 1901 EP 1911 DI 10.1093/molbev/msr011 PG 11 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 766VM UT WOS:000290814600013 PM 21239390 ER PT J AU Moscou, MJ Lauter, N Caldo, RA Nettleton, D Wise, RP AF Moscou, Matthew J. Lauter, Nick Caldo, Rico A. Nettleton, Dan Wise, Roger P. TI Quantitative and Temporal Definition of the Mla Transcriptional Regulon During Barley-Powdery Mildew Interactions SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID F-SP HORDEI; DISEASE-RESISTANCE; BLUMERIA-GRAMINIS; GENE-EXPRESSION; BASAL DEFENSE; FUNGUS; LOCUS; RAR1; ACCUMULATION; DEPENDENCE AB Barley Mildew resistance locus a (Mla) is a major determinant of immunity to the powdery mildew pathogen, Blumeria graminis f. sp. hordei. Alleles of Mla encode cytoplasmic- and membrane-localized coiled-coil, nucleotide binding site, leucine-rich repeat proteins that mediate resistance when complementary avirulence effectors (AVR(a)) are present in the pathogen. Presence of an appropriate AVR(a) protein triggers nuclear relocalization of MLA, in which MLA binds repressing host transcription factors. Time-course expression profiles of plants harboring Mla1, Mla6, and Mla12 wild-type alleles versus paired loss-of-function mutants were compared to discover conserved transcriptional targets of MLA and downstream signaling cascades. Pathogen-dependent gene expression was equivalent or stronger in susceptible plants at 20 h after inoculation (HAI) and was attenuated at later timepoints, whereas resistant plants exhibited a time-dependent strengthening of the transcriptional response, increasing in both fold change and the number of genes differentially expressed. Deregulation at 20 HAI implicated 16 HAI as a crucial point in determining the future trajectory of this interaction and was interrogated by quantitative analysis. In total, 28 potential transcriptional targets of the MLA regulon were identified. These candidate targets possess a diverse set of predicted functions, suggesting that multiple pathways are required to mediate the hypersensitive reaction. C1 [Moscou, Matthew J.; Nettleton, Dan; Wise, Roger P.] Iowa State Univ, Interdept Program Bioinformat & Computat Biol, Ames, IA 50011 USA. [Moscou, Matthew J.; Lauter, Nick; Caldo, Rico A.; Wise, Roger P.] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA. [Lauter, Nick; Wise, Roger P.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA 50011 USA. [Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. RP Wise, RP (reprint author), Iowa State Univ, Interdept Program Bioinformat & Computat Biol, Ames, IA 50011 USA. EM rpwise@iastate.edu RI Moscou, Matthew/D-5266-2011 OI Moscou, Matthew/0000-0003-2098-6818 FU National Science Foundation [0500461]; USDA-Initiative for Future Agriculture and Food Systems [2001-52100-11346]; USDA-ARS CRIS [3625-21000-049-00D] FX The authors thank A. Bogdanove for critical review of the manuscript, J. Peng (Iowa State University Gene Chip facility) for his expertise in performing the Gene Chip hybridizations, and G. Fuerst for preparation of the tables and figures. This article is a joint contribution of The Iowa Agriculture and Home Economics Experiment Station and the Corn Insects and Crop Genetics Research Unit, USDA-ARS. This work was supported by National Science Foundation-Plant Genome Award 0500461 to R. Wise and D. Nettleton, USDA-Initiative for Future Agriculture and Food Systems grant 2001-52100-11346 to R. Wise, and USDA-ARS CRIS #3625-21000-049-00D to R. Wise and N. Lauter. All germplasm identifiers using a Cl prefix refer to the nomenclature applied by the USDA National Plant Germplasm System. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 50 TC 16 Z9 16 U1 0 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JUN PY 2011 VL 24 IS 6 BP 694 EP 705 DI 10.1094/MPMI-09-10-0211 PG 12 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA 767TM UT WOS:000290879600007 PM 21323465 ER PT J AU Suarez, CE Laughery, JM Bastos, RG Johnson, WC Norimine, J Asenzo, G Brown, WC Florin-Christensen, M Goff, WL AF Suarez, Carlos E. Laughery, Jacob M. Bastos, Reginaldo G. Johnson, Wendell C. Norimine, Junzo Asenzo, Gustavo Brown, Wendy C. Florin-Christensen, Monica Goff, Will L. TI A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by Babesia bovis merozoites SO PARASITOLOGY LA English DT Article DE Apicomplexan; Babesia bovis; rhoptry-associated protein-1; RAP-1 ID RHOPTRY-ASSOCIATED PROTEIN-1; LINKED-IMMUNOSORBENT-ASSAY; INVITRO CULTIVATION; SURFACE-PROTEINS; GENOME SEQUENCE; THEILERIA-PARVA; POTENTIAL USE; ANTIBODIES; CATTLE; EPITOPES AB Objective. The Babesia bovis genome encodes a rap-1 related gene denominated RAP-1 related antigen (RRA). In this study, we analysed the pattern of expression, immunogenicity and functional relevance of RRA. Methods. Phylogenetic analysis was performed using the program Phylip. Expression of rra was analysed by Northern blots, RT-PCR, immunoprecipitation, Western blots and immunofluorescence. RRA antigenicity was tested by T-cell proliferation and Western blot analysis, and functional relevance was determined in an in vitro neutralization assay. Results. RRA is more closely related to RAP-1b of Babesia bigemina than to B. bovis RAP-1, and it is highly conserved among distinct strains. Transcriptional analysis suggests lower numbers of rra transcripts compared to rap-1. Immunoprecipitation of metabolically labelled B. bovis proteins with antibodies against synthetic peptides representing predicted antigenic regions of RRA confirmed the expression of a similar to 43 kDa RRA in cultured merozoites. Antibodies present in B. bovis hyperimmune sera, but not in field-infected cattle sera, reacted weakly with recombinant RRA, and no significant stimulation was obtained using recombinant RRA as antigen in T-cell proliferation assays, indicating that RRA is a subdominant antigen. Antibodies against RRA synthetic peptides reacted with merozoites using immunofluorescence, and were able to significantly inhibit erythrocyte invasion in in vitro neutralization tests, suggesting functional relevance for parasite survival. Conclusion. B. bovis express a novel subdominant RAP-1-like molecule that may contribute to erythrocyte invasion and/or egression by the parasite. C1 [Suarez, Carlos E.; Johnson, Wendell C.; Goff, Will L.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Suarez, Carlos E.; Laughery, Jacob M.; Bastos, Reginaldo G.; Norimine, Junzo; Brown, Wendy C.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Brown, Wendy C.] Washington State Univ, Sch Global Anim Hlth, Pullman, WA USA. [Asenzo, Gustavo; Florin-Christensen, Monica] Ctr Invest Ciencias Vet & Agr, Inst Nacl Tecnol Agr, Castelar, Argentina. RP Suarez, CE (reprint author), ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. EM ces@vetmed.wsu.edu NR 40 TC 4 Z9 4 U1 1 U2 5 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 J9 PARASITOLOGY JI Parasitology PD JUN PY 2011 VL 138 IS 7 BP 809 EP 818 DI 10.1017/S0031182011000321 PG 10 WC Parasitology SC Parasitology GA 766EE UT WOS:000290763000001 PM 21554842 ER PT J AU Alvarado-Esquivel, C Urbina-Alvarez, JD Estrada-Martinez, S Torres-Castorena, A Molotla-de-Leon, G Liesenfeld, O Dubey, JP AF Alvarado-Esquivel, Cosme David Urbina-Alvarez, Jesus Estrada-Martinez, Sergio Torres-Castorena, Alejandro Molotla-de-Leon, Gabriel Liesenfeld, Oliver Dubey, Jitender P. TI Toxoplasma gondii infection and schizophrenia: A case control study in a low Toxoplasma seroprevalence Mexican population SO PARASITOLOGY INTERNATIONAL LA English DT Article DE Toxoplasma gondii; Schizophrenia; Case control study; Seroprevalence; Epidemiology ID SEROEPIDEMIOLOGY; DURANGO; ANTIBODIES; INDIVIDUALS AB There are conflicting reports concerning the association of Toxoplasma gondii infection and schizophrenia in humans. Therefore, we determined such association in a Mexican population of Mestizo ethnicity. Through a case-control study design, 50 schizophrenic patients and 150 control subjects matched by gender, age, residence place, and ethnicity were examined with enzyme-linked immunoassays for the presence and levels of T. gondii IgG antibodies and for the presence of T. gondii IgM antibodies. Schizophrenic patients attended a public psychiatric hospital in Durango City, Mexico, and the control group consisted of individuals of the general population of the same city. Socio-demographic, clinical and behavioral characteristics from the study subjects were also obtained. Both the seroprevalence and the level of T. gondii IgG antibodies were higher in schizophrenic patients (10/50; 20%) than in control subjects (8/150; 5.3%) (OR=4.44; 95% CI: 1.49-13.37; P=0.003). The IgG T. gondii levels higher than 150 IU/ml were more frequently observed in patients than in controls (10% versus 2%, respectively; P=0.02). One (50%) of the two patients with recently diagnosed schizophrenia and none of the controls had T. gondii IgM antibodies (P=0.01). T. gondii seropositivity was significantly higher in patients with a history of cleaning cat excrement (P=0.005), and suffering from simple schizophrenia (ICD-10 classification: F20.6) (P=0.03) than patients without these characteristics. Toxoplasma seroprevalence was also significantly higher in patients with simple schizophrenia (F20.6) than in those with paranoid schizophrenia (F20.0) (P=0.02). This study provides elements to clarify the controversial information on the association of T. gondii infection and schizophrenia. Published by Elsevier Ireland Ltd. C1 [Dubey, Jitender P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Estrada-Martinez, Sergio] Juarez Univ Durango State, Inst Sci Res, Durango 34000, Dgo, Mexico. [David Urbina-Alvarez, Jesus; Torres-Castorena, Alejandro] Mexican Social Secur Inst, Durango City 34000, Mexico. [Molotla-de-Leon, Gabriel] Hosp Mental Hlth Dr Miguel Vallebueno, Durango 34237, Dgo, Mexico. [Liesenfeld, Oliver] Charite, Inst Microbiol & Hyg, D-12203 Berlin, Germany. RP Dubey, JP (reprint author), ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM alvaradocosme@yahoo.com; bruss_23@hotmail.com; sem@mexico.com; aletorcas@yahoo.com.mx; gabrielmolotla@yahoo.com.mx; olitoxo@yahoo.com; JITENDER.DUBEY@ARS.USDA.GOV FU Fondos Mixtos Durango-Consejo Nacional de Ciencia y Tecnologia, Mexico [66718] FX This study was supported by Fondos Mixtos Durango-Consejo Nacional de Ciencia y Tecnologia, Mexico, grant no. 66718. NR 32 TC 28 Z9 30 U1 5 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 1383-5769 J9 PARASITOL INT JI Parasitol. Int. PD JUN PY 2011 VL 60 IS 2 BP 151 EP 155 DI 10.1016/j.parint.2010.12.003 PG 5 WC Parasitology SC Parasitology GA 764RY UT WOS:000290651500006 PM 21292026 ER PT J AU Orellana, RA Wilson, FA Gazzaneo, MC Suryawan, A Davis, TA Nguyen, HV AF Orellana, Renan A. Wilson, Fiona A. Gazzaneo, Maria C. Suryawan, Agus Davis, Teresa A. Nguyen, Hanh V. TI Sepsis and Development Impede Muscle Protein Synthesis in Neonatal Pigs by Different Ribosomal Mechanisms SO PEDIATRIC RESEARCH LA English DT Article ID SKELETAL-MUSCLE; TRANSLATION INITIATION; AMINO-ACIDS; INSULIN; ENDOTOXEMIA; STIMULATION; ACTIVATION; MODULATION; RESPONSES; LIVER AB In muscle, sepsis reduces protein synthesis (MPS) by restraining translation in neonates and adults. Even though protein accretion decreases with development as neonatal MPS rapidly declines by maturation, the changes imposed by development on the sepsis-associated decrease in MPS have not been described. Pigs at 7 and 26 d of age were infused. for 8 h with lipopolysaccharide (LPS, endotoxin, 0 and 10 mu g . kg(-1) . h(-1)). Fractional MPS rates and translation eukaryotic initiation factor (eIF) activation in muscle were examined (n = 5-7/group). The LPS-induced decrease in MPS was associated with reduced ribosomal and translational efficiency, whereas the age-induced decrease in MPS occurred by decreasing ribosome number. Abundances of mammalian target of rapamycin (mTOR) and S6 decreased, and that of the repressor eIF4E . 4E-binding protein 1 (4EBP1) association increased in 26-d-old pigs compared with 7-d-old pigs. LPS decreased the abundance of the active eIF4E . eIF4G association and the phosphorylation of eIF4G across ages, whereas the abundance of eIF4G declined and eIF2 alpha phosphorylation increased with age. Therefore, when lacking anabolic stimulation, the decrease in MPS induced by LPS is associated with reduced ribosomal efficiency and decreased eIF4E . eIF4G assembly, whereas that induced by development involves reduced ribosomal number, translation factor abundance, and increased eIF2 alpha phosphorylation. (Pediatr Res 69: 473-478, 2011) C1 [Orellana, Renan A.; Wilson, Fiona A.; Gazzaneo, Maria C.; Suryawan, Agus; Davis, Teresa A.; Nguyen, Hanh V.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. RP Orellana, RA (reprint author), ARS, USDA, Childrens Nutr Res Ctr, 1100 Bates St,Room 9057, Houston, TX 77030 USA. EM orellana@bcm.edu FU NIH [K08 AR-51563, AR-44474]; US Department of Agriculture, Agricultural Research Service [58-6250-6-001] FX Supported by NIH awards K08 AR-51563 [R.A.O.] and AR-44474 [T.A.D.], and the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement number 58-6250-6-001 [T.A.D.]. NR 34 TC 5 Z9 5 U1 0 U2 3 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 W CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD JUN PY 2011 VL 69 IS 6 BP 473 EP 478 DI 10.1203/PDR.0b013e3182176da1 PG 6 WC Pediatrics SC Pediatrics GA 767CA UT WOS:000290831700002 PM 21364490 ER PT J AU Gent, DH De Wolf, E Pethybridge, SJ AF Gent, David H. De Wolf, Erick Pethybridge, Sarah J. TI Perceptions of Risk, Risk Aversion, and Barriers to Adoption of Decision Support Systems and Integrated Pest Management: An Introduction SO PHYTOPATHOLOGY LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Phytopathological-Society CY JUL 31-AUG 05, 2009 CL Portland, OR SP Amer Phytopathol Soc ID PLANT-DISEASE PREDICTION; CROP PROTECTION; IMPLEMENTATION; PYRETHRUM; PROSPECTS AB Rational management of plant diseases, both economically and environmentally, involves assessing risks and the costs associated with both correct and incorrect tactical management decisions to determine when control measures are warranted. Decision support systems can help to inform users of plant disease risk and thus assist in accurately targeting events critical for management. However, in many instances adoption of these systems for use in routine disease management has been perceived as slow. The under-utilization of some decision support systems is likely due to both technical and perception constraints that have not been addressed adequately during development and implementation phases. Growers' perceptions of risk and their aversion to these perceived risks can be reasons for the "slow" uptake of decision support systems and, more broadly, integrated pest management (IPM). Decision theory provides some tools that may assist in quantifying and incorporating subjective and/or measured probabilities of disease occurrence or crop loss into decision support systems. incorporation of subjective probabilities into IPM recommendations may be one means to reduce grower uncertainty and improve trust of these systems because management recommendations could be explicitly informed by growers' perceptions of risk and economic utility. Ultimately though, we suggest that an appropriate measure of the value and impact of decision support systems is grower education that enables more skillful and informed management decisions independent of consultation of the support tool outputs. C1 [Gent, David H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [De Wolf, Erick] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Pethybridge, Sarah J.] Bot Resources Australia Agr Serv Pty Ltd, Ulverstone, Tas 7315, Australia. RP Gent, DH (reprint author), USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu NR 27 TC 27 Z9 27 U1 3 U2 34 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 BP 640 EP 643 DI 10.1094/PHYTO-04-10-0124 PG 4 WC Plant Sciences SC Plant Sciences GA 766ZF UT WOS:000290824400001 PM 21117876 ER PT J AU Pfender, WF Gent, DH Mahaffee, WF Coop, LB Fox, AD AF Pfender, W. F. Gent, D. H. Mahaffee, W. F. Coop, L. B. Fox, A. D. TI Decision Aids for Multiple-Decision Disease Management as Affected by Weather Input Errors SO PHYTOPATHOLOGY LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Phytopathological-Society CY JUL 31-AUG 05, 2009 CL Portland, OR SP Amer Phytopathol Soc ID RYEGRASS SEED CROPS; PERENNIAL RYEGRASS; STEM RUST; WETNESS DURATION; UNCERTAINTY; PREDICTION; BLIGHT; RISK; PROTECTION; FORECAST AB Many disease management decision support systems (DSSs) rely, exclusively or in part, on weather inputs to calculate an indicator for disease hazard. Error in the weather inputs, typically due to forecasting, interpolation, or estimation from off-site sources, may affect model calculations and management decision recommendations. The extent to which errors in weather inputs affect the quality of the final management outcome depends on a number of aspects of the disease management context, including whether management consists of a single dichotomous decision, or of a multi-decision process extending over the cropping season(s). Decision aids for multi-decision disease management typically are based on simple or complex algorithms of weather data which may be accumulated over several days or weeks, It is difficult to quantify accuracy of multi-decision DSSs due to temporally overlapping disease events, existence of more than one solution to optimizing the outcome, opportunities to take later recourse to modify earlier decisions, and the ongoing, complex decision process in which the DSS is only one component. One approach to assessing importance of weather input errors is to conduct an error analysis in which the DSS outcome from high-quality weather data is compared with that from weather data with various levels of bias and/or variance from the original data. We illustrate this analytical approach for two types of DSS, an infection risk index for hop powdery mildew and a simulation model for grass stem rust. Further exploration of analysis methods is needed to address problems associated with assessing uncertainty in multi-decision DSSs. C1 [Pfender, W. F.; Gent, D. H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Pfender, W. F.; Gent, D. H.; Mahaffee, W. F.; Coop, L. B.] Oregon State Univ Bot & Plant Pathol, Corvallis, OR USA. [Mahaffee, W. F.] USDA ARS Hort Crops Res Unit, Corvallis, OR USA. [Fox, A. D.] Fox Weather LLC, Fortuna, CA USA. RP Pfender, WF (reprint author), USDA ARS, Forage Seed & Cereal Res Unit, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM pfenderw@onid.orst.edu NR 32 TC 9 Z9 9 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 BP 644 EP 653 DI 10.1094/PHYTO-05-10-0131 PG 10 WC Plant Sciences SC Plant Sciences GA 766ZF UT WOS:000290824400002 PM 21091182 ER PT J AU Burlakoti, RR Neate, SM Adhikari, TB Gyawali, S Salas, B Steffenson, BJ Schwarz, PB AF Burlakoti, Rishi R. Neate, Stephen M. Adhikari, Tika B. Gyawali, Sanjaya Salas, Bacilio Steffenson, Brian J. Schwarz, Paul B. TI Trichothecene Profiling and Population Genetic Analysis of Gibberella zeae from Barley in North Dakota and Minnesota SO PHYTOPATHOLOGY LA English DT Article DE Fusarium graminearum; Hordeum vulgare; population genetics ID FUSARIUM-GRAMINEARUM CLADE; UNITED-STATES; MYCOTOXIN PROFILES; WHEAT FIELDS; SUGAR-BEET; DIVERSITY; SCAB; PSEUDOGRAMINEARUM; DEOXYNIVALENOL; RECOMBINATION AB Gibberella zeae, the principal cause of Fusarium head blight (FHB) of barley, contaminates grains with several mycotoxins, which creates a serious problem for the malting barley industry in the United States, China, and Europe. However, limited studies have been conducted on the trichothecene profiles and population genetic structure of G. zeae isolates collected from barley in the United States. Trichothecene biosynthesis gene (TRI)-based polymerase chain reaction (PCR) assays and 10 variable number tandem repeat (VNTR) markers were used to determine the genetic diversity and compare the trichothecene profiles of an older population (n = 115 isolates) of G. zeae collected in 1997 to 2000 with a newer population (n = 147 isolates) collected in 2008. Samples were from across the major barley-growing regions in North Dakota and Minnesota. The results of TRI-based PCR assays were further validated using a subset of 32 and 28 isolates of G. zeae by sequence analysis and gas chromatography, respectively. TRI-based PCR assays revealed that all the G. zeae isolates in both populations had markers for deoxynivalenol (DON), and the frequencies of isolates with a 3-acetyldeoxynivalenol (3-ADON) marker in the newer population were approximate to 11-fold higher than those among isolates in the older population. G. zeae populations from barley in the Midwest of the United States showed no spatial structure, and all the isolates were solidly in clade 7 of G. zeae, which is quite different from other barley-growing areas of world, where multiple species of G. woe are commonly found in close proximity and display spatial structure. VNTR analysis showed high gene diversity (H = 0.82 to 0.83) and genotypic diversity but low linkage disequilibrium (LD = 0.02 to 0.07) in both populations. Low genetic differentiation (F(ST) = 0.013) and high gene flow (Nm = 36.84) was observed between the two populations and among subpopulations within the same population (Nm = 12.77 to 29.97), suggesting that temporal and spatial variations had little influence on population differentiation in the Upper Midwest. Similarly, tow F(ST) (0.02) was observed between 3-ADON and 15-acetyldeoxynivalenol populations, indicating minor influence of the chemotype of G. zeae isolates on population subdivision, although there was a rapid increase in the frequencies of isolates with the 3-ADON marker in the Upper Midwest between the older collection made in 1997 to 2000 and the newer collection made in 2008. This study provides information to barley-breeding programs for their selection of isolates of G. zeae for evaluating barley genotypes for resistance to FHB and DON accumulation. C1 [Burlakoti, Rishi R.; Neate, Stephen M.; Adhikari, Tika B.; Gyawali, Sanjaya] N Dakota State Univ NDSU Dept 7660, Dept Plant Pathol, Fargo, ND 58108 USA. [Salas, Bacilio] USDA, Pest Detect Diagnost & Management Lab, Edinburg, TX 78541 USA. [Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Schwarz, Paul B.] NDSU Dept 7670, Dept Plant Sci, Fargo, ND USA. RP Neate, SM (reprint author), Agrisci Queensland DEEDI, POB 2282, Toowoomba, Qld 4350, Australia. EM Stephen.Neate@DEEDI.qld.gov.au OI Steffenson, Brian/0000-0001-7961-5363 FU American Malting Barley Association; North Dakota Barley Council FX We thank K. O'Donnell (USDA) for providing representative strains of the G. Zeae, H. Chawla for technical assistance in laboratory work, and S. Mamidi for his valuable suggestions in data analysis. This work was funded by a grant from the American Malting Barley Association and the collection of barley samples was funded by the North Dakota Barley Council. NR 52 TC 16 Z9 17 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 BP 687 EP 695 DI 10.1094/PHYTO-04-10-0101 PG 9 WC Plant Sciences SC Plant Sciences GA 766ZF UT WOS:000290824400006 PM 21244225 ER PT J AU Wang, DP Korban, SS Pusey, PL Zhao, YF AF Wang, Dongping Korban, Schuyler S. Pusey, P. Lawrence Zhao, Youfu TI Characterization of the RcsC Sensor Kinase from Erwinia amylovora and Other Enterobacteria SO PHYTOPATHOLOGY LA English DT Article DE fire blight; polysaccharide; regulation ID ENTERICA SEROVAR TYPHIMURIUM; ESCHERICHIA-COLI K-12; PHOSPHORELAY SYSTEM; SWARMING MOTILITY; BIOFILM FORMATION; PATHOGENICITY; VIRULENCE; EXOPOLYSACCHARIDE; BIOSYNTHESIS; INFECTION AB RcsC is a hybrid sensor kinase which contains a sensor domain, a histidine kinase domain, and a receiver domain. We have previously demonstrated that, although the Erwinia amylovora rcsC mutant produces more amylovoran than the wild-type (WT) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. In this study, we have comparatively characterized the Erwinia RcsC and its homologs from various enterobacteria. Results demonstrate that expression of the Erwinia rcsC gene suppresses amylovoran production in various amylovoran overproducing WT and mutant strains, thus suggesting the presence of a net phosphatase activity of Erwinia RcsC. Findings have also demonstrated that rcsC homologs from other enterobacteria could not rescue amylovoran production of the Erwinia rcsC mutant in vitro. However, virulence of the Erwinia rcsC mutant is partially restored by rcsC homologs from Pantoea stewartii, Yersinia pestis, and Salmonella enterica but not from Escherichia coli on apple shoots. Domain-swapping experiments have indicated that replacement of the E. coli RcsC sensor domain by those of Erwinia and Yersinia spp. partially restores virulence of the Erwinia rcsC mutant, whereas chimeric constructs containing the sensor domain of E. coli RcsC could not rescue virulence of the Erwinia rcsC mutant on apple. Interestingly, only chimeric constructs containing the histidine kinase and receiver domains of Erwinia RcsC are fully capable of rescuing amylovoran production. These results suggest that the sensor domain of RcsC may be important in regulating bacterial virulence, whereas the activity of the histidine kinase and receiver domains of Erwinia RcsC may be essential for amylovoran production in vitro. C1 [Wang, Dongping; Zhao, Youfu] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Korban, Schuyler S.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Pusey, P. Lawrence] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Zhao, YF (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM zhao888@illinois.edu RI Wang, Dongping /L-2124-2013 FU United States Department of Agriculture (USDA) National Institute of Food and Agriculture [2010-65110-20497]; USDA-SCRI [AG 2009-51181-06023] FX We thank D. Clarke for providing cloning vector and several rcsC constructs and J. M. Slauch and X. Li for providing genomic DNA from Salmonella enteric and Yersinia pestis, respectively. This project was supported by the Agriculture and Food Research Initiative Competitive Grants Program grant no. 2010-65110-20497 from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture to Y. F. Zhao and USDA-SCRI grant AG 2009-51181-06023 to S. S. Korban. NR 31 TC 13 Z9 13 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 BP 710 EP 717 DI 10.1094/PHYTO-09-10-0258 PG 8 WC Plant Sciences SC Plant Sciences GA 766ZF UT WOS:000290824400008 PM 21261468 ER PT J AU Domier, LL Hobbs, HA McCoppin, NK Bowen, CR Steinlage, TA Chang, S Wang, Y Hartman, GL AF Domier, Leslie L. Hobbs, Houston A. McCoppin, Nancy K. Bowen, Charles R. Steinlage, Todd A. Chang, Sungyul Wang, Yi Hartman, Glen L. TI Multiple Loci Condition Seed Transmission of Soybean mosaic virus (SMV) and SMV-Induced Seed Coat Mottling in Soybean SO PHYTOPATHOLOGY LA English DT Article DE quantitative trait loci; RNA silencing; virus movement ID HC-PRO PROTEIN; TO-CELL MOVEMENT; GLYCINE-MAX; QTL-ANALYSIS; PEA EMBRYO; SMALL RNAS; RESISTANCE; GENE; ARABIDOPSIS; PLANTS AB Infection of soybean plants with Soybean mosaic virus (SMV), which is transmitted by aphids and through seed, can cause significant reductions in seed production and quality. Because seedborne infections are the primary sources of inoculum for SMV infections in North America, host-plant resistance to seed transmission can limit the pool of plants that can serve as sources of inoculum. To examine the inheritance of SMV seed transmission in soybean, crosses were made between plant introductions (PIs) with high (PI88799), moderate (PI60279), and low (PI548391) rates of transmission of SMV through seed. In four F(2) populations, SMV seed transmission segregated as if conditioned by two or more genes. Consequently, a recombinant inbred line population was derived from a cross between PIs 88799 and 548391 and evaluated for segregation of SMV seed transmission, seed coat mottling, and simple sequence repeat markers. Chromosomal regions on linkage groups C1 and C2 were significantly associated with both transmission of isolate SMV 413 through seed and SMV-induced seed coat mottling, and explained approximate to 42.8 and 46.4% of the variability in these two traits, respectively. Chromosomal regions associated with seed transmission and seed coat mottling contained homologues of Arabidopsis genes DCL3 and RDR6, which encode enzymes involved in RNA-mediated transcriptional and posttranscriptional gene silencing. C1 [Domier, Leslie L.; McCoppin, Nancy K.; Bowen, Charles R.; Hartman, Glen L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Domier, Leslie L.; Hobbs, Houston A.; McCoppin, Nancy K.; Bowen, Charles R.; Steinlage, Todd A.; Chang, Sungyul; Wang, Yi; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Domier, LL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM leslie.domier@ars.usda.gov FU United States Department of Agriculture Cooperative State Research, Education, and Extension and Agricultural Research Services; Illinois Soybean Association FX Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the United States Department of Agriculture or the University of Illinois and does not imply its approval to the exclusion of other products or vendors that may also be suitable. This work was supported by the United States Department of Agriculture Cooperative State Research, Education, and Extension and Agricultural Research Services and the Illinois Soybean Association. We thank J. Davenport, M. McAllister, and D. Poly-chronopoulos for their assistance in genetic analysis of soybean populations; and R. Warsaw, L. Crull, and A. Waymire for their assistance in the field experiments. NR 73 TC 13 Z9 16 U1 1 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2011 VL 101 IS 6 BP 750 EP 756 DI 10.1094/PHYTO-09-10-0239 PG 7 WC Plant Sciences SC Plant Sciences GA 766ZF UT WOS:000290824400013 PM 21561316 ER PT J AU Polley, HW Fay, PA Jin, VL Combs, GF AF Polley, H. Wayne Fay, Philip A. Jin, Virginia L. Combs, Gerald F., Jr. TI CO2 enrichment increases element concentrations in grass mixtures by changing species abundances SO PLANT ECOLOGY LA English DT Article DE Bouteloua curtipendula; C-4 grasses; Soil type; Sorghastrum nutans; Subambient CO2 concentration; Tallgrass prairie ID ELEVATED ATMOSPHERIC CO2; TALLGRASS PRAIRIE; PLANT-TISSUES; PRODUCTIVITY; SUBAMBIENT; VEGETATION; RESPONSES; CARBON; AVAILABILITY; NUTRITION AB Atmospheric carbon dioxide (CO2) enrichment may increase plant growth more than the uptake of chemical elements from soil. Increased CO2 also may alter element levels in biomass from multi-species vegetation by changing plant species abundances. We measured concentrations of ten elements in aboveground tissues of three C-4 grasses that had been exposed for 2-3 growing seasons to a continuous gradient in CO2 from 250 to 500 mu mol mol(-1). The grasses, Bouteloua curtipendula, Schizachyrium scoparium, and Sorghastrum nutans, are competitive dominants in assemblages of tallgrass prairie vegetation growing on each of three soil types along a field CO2 gradient in central Texas, USA. Our objective was to determine whether CO2 influences element concentrations in grass mixtures by changing concentrations in individual species or shifting species abundances. Increased CO2 had little effect on element concentrations in grasses compared to differences observed among grass species and soils. Increasing CO2 from the pre-Industrial to elevated levels reduced the phosphorus concentration in grasses grown on a clay and sandy loam soil. Concentrations of most other elements did not respond to CO2 treatment. Cover of the mid-grass Bouteloua declined at higher CO2 levels as cover of the taller grass Sorghastrum increased. Concentrations of several elements were lower in Bouteloua than Sorghastrum; hence, this exchange of species at higher CO2 increased element concentrations in grass assemblages. Potential consequences include an improvement in the nutritional quality of plants for herbivores. Results highlight the underappreciated impact that CO2 enrichment may have on ecosystem functioning by changing plant composition. C1 [Polley, H. Wayne; Fay, Philip A.; Jin, Virginia L.] Agr Res Serv, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. [Combs, Gerald F., Jr.] Agr Res Serv, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58202 USA. RP Polley, HW (reprint author), Agr Res Serv, Grassland Soil & Water Res Lab, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. EM wayne.polley@ars.usda.gov OI Fay, Philip/0000-0002-8291-6316 NR 34 TC 11 Z9 14 U1 1 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD JUN PY 2011 VL 212 IS 6 BP 945 EP 957 DI 10.1007/s11258-010-9874-y PG 13 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 765FB UT WOS:000290687700002 ER PT J AU Miles, DM Rowe, DE Cathcart, TC AF Miles, D. M. Rowe, D. E. Cathcart, T. C. TI Litter ammonia generation: Moisture content and organic versus inorganic bedding materials SO POULTRY SCIENCE LA English DT Article DE ammonia; bedding; broiler; litter; moisture content ID REARING BROILER-CHICKENS; CARBON-DIOXIDE; POULTRY LITTER; VOLATILIZATION; SAND; PERFORMANCE; CAKE AB Negative impacts on the environment, bird well-being, and farm worker health indicate the need for abatement strategies for poultry litter NH3 generation. Type of bedding affects many parameters related to poultry production including NH3 losses. In a randomized complete block design, 3 trials compared the cumulative NH3 volatilization for laboratory-prepared litter (4 bedding types mixed with excreta) and commercial litter (sampled from a broiler house during the second flock on reused pine wood chips). Litters were assessed at the original moisture content and 2 higher moisture contents. Broiler excrement was mixed with pine wood shavings, rice hulls, sand, and vermiculite to create litter samples. Volumetrically uniform litter samples were placed in chambers receiving humidified air where the exhaust passed through H3BO3 solution, trapping litter-emitted NH3. At the original moisture content, sand and vermiculite litters generated the most NH3 (5.3 and 9.1 mg of N, respectively) whereas wood shavings, commercial, and rice hull litters emitted the least NH3 (0.9-2.6 mg of N). For reducing NH3 emissions, the results support recommendations for using wood shavings and rice hulls, already popular bedding choices in the United States and worldwide. In this research, the organic bedding materials generated the least NH3 at the original moisture content when compared with the inorganic materials. For each bedding type, incremental increases in litter moisture content increased NH3 volatilization. However, the effects of bedding material on NH3 volatilization at the increased moisture levels were not clearly differentiated across the treatments. Vermiculite generated the most NH3 (26.3 mg of N) at the highest moisture content. Vermiculite was a novel bedding choice that has a high water absorption capacity, but because of high NH3 generation, it is not recommended for further study as broiler bedding material. Controlling unnecessary moisture inputs to broiler litter is a key to controlling NH3 emissions. C1 [Miles, D. M.] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. [Rowe, D. E.] Mississippi State Univ, Dept Expt Stat, Mississippi State, MS 39762 USA. [Cathcart, T. C.] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. RP Miles, DM (reprint author), ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. EM dana.miles@ars.usda.gov NR 25 TC 15 Z9 18 U1 3 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN 1 PY 2011 VL 90 IS 6 BP 1162 EP 1169 DI 10.3382/ps.2010-01113 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 766BE UT WOS:000290752600003 PM 21597054 ER PT J AU Meydan, H Yildiz, MA Dodgson, JB Cheng, HH AF Meydan, H. Yildiz, M. A. Dodgson, J. B. Cheng, H. H. TI Allele-specific expression analysis reveals CD79B has a cis-acting regulatory element that responds to Marek's disease virus infection in chickens SO POULTRY SCIENCE LA English DT Article DE Marek's disease; genetic resistance; allele-specific expression; CD79B; growth hormone ID AFFECTING SUSCEPTIBILITY; GENE-EXPRESSION; IDENTIFICATION; RESISTANCE; COMPLEX; PROTEIN; SCREEN; CELLS AB Marek's disease (MD) is a T cell lymphoma disease of domestic chickens induced by the Marek's disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus. Enhancing genetic resistance to MD in poultry is an attractive method to augment MD vaccines, which protect against MD but do not prevent MDV replication and horizontal spread. Previous work integrating QTL scans, transcript profiling, and MDV-chicken protein-protein interaction screens revealed 3 MD resistance genes; however, a major challenge continues to be the identification of the other contributing genes. To aid in this search, we screened for allele-specific expression (ASE) in response to MDV infection, a simple and novel method for identifying polymorphic cis-acting regulatory elements, which may contain strong candidate genes with specific alleles that confer MD genetic resistance. In this initial study, we focused on immunoglobulin beta (CD79B) because it plays a critical role in the immune response and, more important, is transcriptionally coupled with growth hormone (GH1), one of the previously identified MD resistance genes. Using a coding SNP in CD79B and pyrosequencing to track the relative expression of each allele, we monitored ASE in uninfected and MDV-infected F(1) progeny from reciprocal intermatings of highly inbred chicken lines 6(3) (MD resistant) and 7(2) (MD susceptible). Upon screening 3 tissues (bursa, thymus, and spleen) at 5 time points (1, 4, 7, 11, and 15 d postinfection), we observed that MDV infection alters the CD79B allelic ratios in bursa and thymus tissues at 4 and 15 d postinfection in both mating directions. Our results suggest that CD79B has a cis-acting regulatory element that responds to MDV infection and probably cooperates with GH1 in conferring genetic resistance to MD. This result helps validates the use of ASE screens to identify specific candidate genes for complex traits such as genetic resistance to MD. C1 [Cheng, H. H.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Meydan, H.; Yildiz, M. A.] Ankara Univ, Dept Anim Sci, TR-06110 Ankara, Turkey. [Meydan, H.; Dodgson, J. B.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. RP Cheng, HH (reprint author), ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. EM hans.cheng@ars.usda.gov RI MEYDAN, HASAN/C-5003-2016 OI MEYDAN, HASAN/0000-0003-4681-2525 FU USDA National Institute of Food and Agriculture [2007-04201]; Council of Higher Education of Turkey FX We thank Laurie Molitor (USDA, ARS, ADOL, East Lansing, MI) for excellent technical assistance. This work was supported by National Research Initiative Competitive Grant no. 2007-04201 from the USDA National Institute of Food and Agriculture to HHC and The Council of Higher Education of Turkey to HM and MAY. NR 24 TC 8 Z9 8 U1 1 U2 1 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD JUN 1 PY 2011 VL 90 IS 6 BP 1206 EP 1211 DI 10.3382/ps.2010-01295 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 766BE UT WOS:000290752600009 PM 21597060 ER PT J AU Byrd, JA Sams, AR Hargis, BM Caldwell, DJ AF Byrd, J. A. Sams, A. R. Hargis, B. M. Caldwell, D. J. TI Effect of selected modified atmosphere packaging on Campylobacter survival in raw poultry SO POULTRY SCIENCE LA English DT Article DE modified atmosphere; packaging; Campylobacter ID BROILER CARCASSES; SHELF-LIFE; DEGREES-C; CHICKEN; JEJUNI; SPOILAGE; TEMPERATURE; RECOVERY; BACTERIA; STORAGE AB Most current research on Campylobacter has focused on preharvest or processing plant cross-contamination. Little is known about the effect of storage environment on the survival of Campylobacter on raw poultry. We evaluated the effects of modified storage atmosphere and freezing on the survival of naturally occurring Campylobacter on raw poultry. Broiler carcasses (n = 560) were collected as they exited the chiller in 2 commercial processing plants and were sampled for the detection of Campylobacter, Escherichia coli, psychrophiles, and total aerobes at 0 and 14 d of refrigerated (2 degrees C) storage. Gases evaluated were air, 100% O(2), 100% CO(2), and a standard poultry modified atmosphere packaging mixture (5% O(2) + 10% CO(2) + 85% N). Freezing was included as a control group. All carcasses were sampled by the whole-carcass rinse method. The rinse fluid was recovered and pooled from 5 individual rinses, and serial dilutions were made for examination of Campylobacter (42 degrees C, 48 h), E. coli (37 degrees C, 24 h), psychrophiles (plate count agar, 4 degrees C, 7 d), and total aerobic bacterial populations (plate count agar, 37 degrees C, 24 h). Campylobacter counts for all treatments were reduced during the 14-d storage period but the 100% O(2) treatment caused a significantly (P < 0.05) greater reduction than the other gas treatments. For the psychrophiles, storage in air resulted in the greatest growth after 14 d, with reduced psychrophilic growth allowed by either O(2) or the modified atmosphere packaging mixture (not different from each other). Of the treatments evaluated, CO(2) allowed the least growth of psychrophiles. Proliferation of E. coli and aerobes was the greatest when packaged in air after 14 d, whereas CO(2) packaging resulted in the least growth. These data suggest that storage under O(2) may reduce Campylobacter recovery and slow psychrophile and aerobe recovery following storage. C1 [Byrd, J. A.] ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. [Sams, A. R.; Caldwell, D. J.] Texas A&M Univ, Texas Agr Expt Stn, Dept Poultry Sci, College Stn, TX 77843 USA. [Hargis, B. M.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Caldwell, D. J.] Texas A&M Univ, Texas Agr Expt Stn, Dept Vet Pathobiol, College Stn, TX 77843 USA. RP Byrd, JA (reprint author), ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. EM allen.byrd@ars.usda.gov NR 21 TC 5 Z9 5 U1 2 U2 17 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD JUN 1 PY 2011 VL 90 IS 6 BP 1324 EP 1328 DI 10.3382/ps.2010-00746 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA 766BE UT WOS:000290752600023 PM 21597074 ER PT J AU Halvorson, JJ Gonzalez, JM Hagerman, AE AF Halvorson, Jonathan J. Gonzalez, Javier M. Hagerman, Ann E. TI Repeated applications of tannins and related phenolic compounds are retained by soil and affect cation exchange capacity SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Tannins; Sorption; Cation exchange capacity; Soil organic matter; C-sequestration; Tannic acid; Methyl gallate; Gallic acid; beta-1,2,3,4,6-penta-O-galloyl-D-glucose; PGG; Catechin; Procyanidin ID DISSOLVED ORGANIC-MATTER; NUTRIENT DYNAMICS; PROTEIN PRECIPITATION; ADSORPTION-ISOTHERM; NITROGEN DYNAMICS; TEMPERATE FOREST; ALASKAN TAIGA; LEAF-LITTER; CARBON; SORPTION AB Retention of tannins, produced by plants, could be important for managing soil organic matter and nutrient cycling. However, we know little about the comparative retention of different classes of tannins and related compounds or if soils have a maximum storage capacity for them. To address these questions, forest, and pasture loam soils, collected at 0-5 cm (surface) and 10-20 cm (subsurface), were repeatedly treated with water (Control) or solutions containing condensed and hydrolyzable tannins or related phenolic subunits (10 mg g(-1) soil). Treatments included a polymeric flavonoid-based procyanidin from sorghum, catechin, tannic acid, beta-1,2,3,4,6-penta-O-galloyl-D-glucose (PGG), gallic acid, and methyl gallate. After each application, soluble-C in supernatants was determined by oxidative-combustion infrared analysis and retention of treatment-carbon by soil was calculated as the difference between added and recovered soluble-C. An interaction between soil depth and treatment was evident through all applications with highest retention of both hydrophobic (PGG) and hydrophilic (procyanidin) tannins, compared to other phenolic compounds. For all treatments except gallic acid and methyl gallate, higher sorption occurred in surface soil, which contained more organic matter than subsurface soil. With each successive application, less additional treatment-C was retained by soil and the amount of C remaining in supernatants was correlated with the presence of phenolic substances. Cumulative retention by surface soil was more than 10.3, 8.5 and 6.4 mg C g(-1), soil for PGG, tannic acid, and procyanidin, several times higher than the other compounds. Soluble-C extracted from treated soil, with cool water (23 degrees C), was 1-2 orders of magnitude greater than Control samples and highly correlated with Prussian Blue (PB) phenolics, indicating some retained treatment-C was only weakly held on the soil. The final extraction, with hot water (80 degrees C), removed more soluble-C, particularly from surface samples, that contained fewer PB phenolics per unit soluble-C than cool water extracts. After all extractions more than 85% of sorbed procyanidin-C was retained by samples compared to 81% of methyl gallate, 79% of PGG, 74% of tannic acid, 50% of catechin, and 40% of the gallic acid. Total C. measured in soil after all extractions, was close to expected values, confirming tannins and phenolic compounds had remained in soil and were not otherwise lost. Cation exchange capacity was increased about 30% in subsurface and forest samples by PGG, a hydrolyzable tannin, but decreased by 30% and 35% in surface and pasture soil, respectively, by its monomer, gallic acid. Published by Elsevier Ltd. C1 [Halvorson, Jonathan J.; Gonzalez, Javier M.] ARS, USDA, Appalachian Farming Syst Res Ctr, Beaver, WV 25813 USA. [Hagerman, Ann E.] Miami Univ, Oxford, OH 45056 USA. RP Halvorson, JJ (reprint author), ARS, USDA, Appalachian Farming Syst Res Ctr, 1224 Airport Rd, Beaver, WV 25813 USA. EM Jonathan.Halvorson@ars.usda.gov NR 70 TC 12 Z9 12 U1 4 U2 34 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2011 VL 43 IS 6 BP 1139 EP 1147 DI 10.1016/j.soilbio.2011.01.023 PG 9 WC Soil Science SC Agriculture GA 765IF UT WOS:000290698100004 ER PT J AU Somenahally, AC Hollister, EB Loeppert, RH Yan, WG Gentry, TJ AF Somenahally, Anil C. Hollister, Emily B. Loeppert, Richard H. Yan, Wengui Gentry, Terry J. TI Microbial communities in rice rhizosphere altered by intermittent and continuous flooding in fields with long-term arsenic application SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Rice rhizosphere; Pore-water As; Rhizosphere-microbial communities; 16S pyrosequencing; qPCR; Iron-reducing bacteria ID BENGAL DELTA SEDIMENTS; REDUCING BACTERIA; FE(III)-REDUCING BACTERIA; CONTAMINATED SOILS; PADDY SOIL; REDUCTION; IRON; GRAIN; BANGLADESH; DIVERSITY AB Rice cultivated on arsenic (As)-contaminated soils can, under some conditions, accumulate high concentrations of As in grain, mostly as a result of the continuous flooding practices commonly used for rice cultivation. Intermittent flooding, as opposed to continuous flooding, might reduce soluble As concentrations in the rice rhizosphere, but it might also alter soil microbial populations that may impact As chemistry. A field-scale study was conducted to analyze As concentrations and microbial populations in the rice rhizosphere, in response to intermittent and continuous flooding in plots that were historically amended with "As-containing" pesticide and unamended soil. Rhizosphere, pore-water and grain As concentrations were quantified, and microbial populations in the rhizosphere were characterized using community quantitative-PCR and 16S rRNA gene sequencing. Pore-water As concentrations decreased by 41-81% and grain As by 31-48% in the intermittently flooded plots relative to the continuously flooded plots. The relative abundance of bacteria increased over the course of the growing season, while archaeal and fungal gene abundances decreased. Bacterial community structure and composition were significantly different between As amended and unamended plots, as well as between the flooding treatments. Proteobacteria was the predominant phylum detected in most treatments with relative abundance of 24-29%. The relative abundance of iron-reducing bacteria was higher with the continuous flood compared to the intermittent-flood treatment, implying greater relative iron reduction and possibly As release from the iron oxides under the continuously flooded conditions. These differences in rhizosphere-microbial communities may have contributed to the lower pore-water arsenic concentrations in the intermittently flooded conditions. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Somenahally, Anil C.; Hollister, Emily B.; Loeppert, Richard H.; Gentry, Terry J.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Yan, Wengui] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Somenahally, AC (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, 370 Olsen Blvd, College Stn, TX 77843 USA. EM asomenahally@ag.tamu.edu NR 58 TC 23 Z9 24 U1 2 U2 63 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2011 VL 43 IS 6 BP 1220 EP 1228 DI 10.1016/j.soilbio.2011.02.011 PG 9 WC Soil Science SC Agriculture GA 765IF UT WOS:000290698100013 ER PT J AU Uselman, SM Snyder, KA Blank, RR Jones, TJ AF Uselman, Shauna M. Snyder, Keirith A. Blank, Robert R. Jones, Timothy J. TI UVB exposure does not accelerate rates of litter decomposition in a semi-arid riparian ecosystem SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Biological control; Diorhabda carinulata; Great Basin Desert; Insect herbivory; Lepidium latifolium; Litter quality; Nutrient dynamics; Photodegradation; Tamarix spp.; Ultraviolet-B radiation ID ULTRAVIOLET-B RADIATION; LONG-TERM DECOMPOSITION; TIERRA-DEL-FUEGO; LEAF-LITTER; PLANT LITTER; NUTRIENT DYNAMICS; UNITED-STATES; TERRESTRIAL ECOSYSTEMS; CLIMATE-CHANGE; LIGNIN CONTROL AB Aboveground litter decomposition is controlled mainly by substrate quality and climate factors across terrestrial ecosystems, but photodegradation from exposure to high-intensity ultraviolet-B (UVB) radiation may also be important in arid and semi-arid environments. We investigated the interactive effects of UVB exposure and litter quality on decomposition in a Tamarix-invaded riparian ecosystem during the establishment of an insect biological control agent in northern Nevada. Feeding by the northern tamarisk beetle (Diorhabda carinulata) on Tamarix spp. trees leads to altered leaf litter quality and increased exposure to solar UVB radiation from canopy opening. In addition, we examined the dynamics of litter decomposition of the invasive exotic Lepidium latifolium, because it is well-situated to invade beetle-infested Tamarix sites. Three leaf litter types (natural Tamarix, beetle-affected Tamarix, and L latifolium) differing in substrate quality were decomposed in litterbags for one year in the field. Litterbags were subjected to one of three treatments: (1) Ambient UVB or (2) Reduced UVB (where UVB was manipulated by using clear plastic films that transmit or block UVB), and (3) No Cover (a control used to test for the effect of using the plastic films, i.e. a cover effect). Results showed a large cover effect on rates of decomposition and nutrient release, and our findings suggested that frequent cycles of freeze thaw, and possibly rainfall intensity, influenced decomposition at this site. Contrary to our expectations, greater UVB exposure did not result in faster rates of decomposition. Greater UVB exposure resulted in decreased rates of decomposition and P release for the lower quality litter and no change in rates of decomposition and nutrient release for the two higher quality litter types, possibly due to a negative effect of UVB on soil microbes. Among litter types, rates of decomposition and net release of N and P followed this ranking: L latifolium > beetle-affected Tamarix > natural Tamarix. Altered nutrient dynamics with beetle introduction as well as the rapid decomposition rates exhibited by L latifolium are consistent with vulnerability to secondary invasion. In this desert ecosystem, decomposition and nutrient release were strongly affected by litter type and much less so by UVB exposure. Published by Elsevier Ltd. C1 [Uselman, Shauna M.; Snyder, Keirith A.; Blank, Robert R.; Jones, Timothy J.] ARS, USDA, Exot & Invas Weeds Res Unit, Reno, NV 89512 USA. RP Uselman, SM (reprint author), ARS, USDA, Exot & Invas Weeds Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM Shauna.Uselman@ars.usda.gov; Keirith.Snyder@ars.usda.gov; Bob.Blank@ars.usda.gov; Timothy.jones@ars.usda.gov NR 70 TC 31 Z9 33 U1 1 U2 40 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2011 VL 43 IS 6 BP 1254 EP 1265 DI 10.1016/j.soilbio.2011.02.016 PG 12 WC Soil Science SC Agriculture GA 765IF UT WOS:000290698100017 ER PT J AU Gomez, AM Yannarell, AC Sims, GK Cadavid-Restrepo, G Herrera, CXM AF Gomez, Andres M. Yannarell, Anthony C. Sims, Gerald K. Cadavid-Restrepo, Gloria Moreno Herrera, Claudia X. TI Characterization of bacterial diversity at different depths in the Moravia Hill landfill site at Medellin, Colombia SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Bacterial diversity; Depth; Solid waste dump/landfill; Hydrocarbon degradation; Temporal temperature gel electrophoresis; Terminal restriction fragment length polymorphism; Ribosomal intergenic spacer analysis ID 16S RIBOSOMAL-RNA; GRADIENT GEL-ELECTROPHORESIS; FRAGMENT-LENGTH-POLYMORPHISMS; INTERGENIC SPACER ANALYSIS; HORIZONTAL GENE-TRANSFER; MICROBIAL DIVERSITY; COMMUNITY STRUCTURE; HYDROCARBON DEGRADATION; PROKARYOTIC DIVERSITY; CONTAMINATED SOIL AB A combination of culture-dependent and culture-independent methods was used to assess bacterial diversity at different depths within a former solid waste dump in Medellin, Colombia. Sampling sites included a densely populated area, which is built upon 40 m of solid waste (domestic, industrial, agricultural, and medical). The soil and leachate contain high levels of contaminants and the natural soil is highly disturbed with solid anthropogenic materials, disrupting natural aggregation and resulting in a loose, porous matrix with irregular aggregate structure. The unusual physical structure and contaminant levels at the site made it unclear if the indigenous bacterial community would possess the complexity commonly observed for natural soils, and thus may limit potential for remediating the site using monitored natural attenuation. Bacterial diversity patterns were determined through 16S-TTGE and T-RFLP at depths of 0, 10, 20 and 30 m. Abundance and diversity patterns, as estimated by number and intensity of terminal restriction fragments and TTGE bands, varied among the 4 different depths, showing more complex patterns in deeper samples (20 and 30 m), which also contained greater concentrations of organic carbon. General diversity patterns were dominated by the phylum Proteobacteria (lambda, beta, and alpha divisions). These findings were reinforced by analysis of the culturable fraction able to use n-hexadecane as sole carbon source, in which the genera Acinetobacter sp. (lambda-Proteobacteria) was dominant. This research offers new clues regarding bacterial diversity patterns through different depths in polluted environments with unique physicochemical conditions, suggesting that bacterial diversity profiles may be highly influenced by the nature of pollutants present. Additionally, results imply that the culturable fraction at the site has a very important role in the community. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Gomez, Andres M.; Cadavid-Restrepo, Gloria; Moreno Herrera, Claudia X.] Univ Nacl Colombia Medellin, Microbiodivers & Bioprospect Res Grp, Ind Microbiol Lab, Medellin, Colombia. [Gomez, Andres M.; Cadavid-Restrepo, Gloria; Moreno Herrera, Claudia X.] Univ Nacl Colombia Medellin, Cellular & Mol Biol Lab, Medellin, Colombia. [Yannarell, Anthony C.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Sims, Gerald K.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP Gomez, AM (reprint author), Univ Illinois, Inst Genom Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA. EM amgomez@illinois.edu; cxmoreno@unal.edu.co RI Sims, Gerald/A-2500-2008; OI Moreno Herrera, Claudia Ximena/0000-0002-8132-5223 FU Urban development Office; Metropolitan Area in Medellin, Colombia; Agricultural Research Service, United States Department of Agriculture [3611-12220-006-00D]; American Society for Microbiology FX The authors thank Moises Posada, Lynn Connor and Terry Holman for laboratory assistance and Dr. Angela Kent for computing support and advising. This work was supported by The Urban development Office and The Metropolitan Area in Medellin, Colombia, The Agricultural Research Service, United States Department of Agriculture, project number 3611-12220-006-00D and a grant provided by the American Society for Microbiology and its international fellowship program for Latin America and the Caribbean. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture, University of Illinois, or The National University of Colombia. NR 76 TC 6 Z9 7 U1 1 U2 27 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2011 VL 43 IS 6 BP 1275 EP 1284 DI 10.1016/j.soilbio.2011.02.018 PG 10 WC Soil Science SC Agriculture GA 765IF UT WOS:000290698100019 ER PT J AU Kichler, CM Fulton, JP Raper, RL McDonald, TP Zech, WC AF Kichler, C. M. Fulton, J. P. Raper, R. L. McDonald, T. P. Zech, W. C. TI Effects of transmission gear selection on tractor performance and fuel costs during deep tillage operations SO SOIL & TILLAGE RESEARCH LA English DT Article DE Data acquisition; Power; Torque; Tractor performance; Subsoiler; Site-specific ID COASTAL-PLAIN; SOIL; CORN; COMPACTION; YIELD; WATER AB Deep tillage operations required to alleviate soil compaction common in the Southeastern US remain energy intensive and expensive. Equipment performance and in-field efficiency are two variables that can be improved to minimize fuel consumption and ultimately reduce input costs for crop production. The objective of this study was to investigate the effects of transmission gear selection on fuel costs, draft, and other equipment performance variables using two deep tillage implements. Three different transmission gears were selected which represented slow, normal, and fast operating speeds for two typical in-row, integral subsoilers (KMC generation I rip-strip and Bigham Brothers Paratill (TM)). Tractor fuel consumption, slip, axle torque, and engine speed were measured in real-time along with transverse and vertical draft forces. Results indicated a 105% increase in fuel consumption rate, a 28% increase in implement draft, and a 255% increase in power between the slow and fast speed for the Paratill (TM). The KMC showed a 115% increase in fuel consumption rate, a 37% increase in implement draft, and a 283% increase in power between the slow and fast speeds. Good comparisons were found between measured and estimated, using published equations, for fuel consumption (-5.3% to 4.9%) and draft (-3.6% to 17.7%). For the Paratill (TM), the normal speed produced the lowest fuel cost ($ 5.10/ha) but operating at this speed reduced productivity rate from 4.55 ha/h at the fast speed down to 3.23 ha/h. Conversely, the KMC fast speed provided the lowest fuel cost ($ 5.35/ha) and highest productivity rate (4.35 ha/h) for this implement. In general, the Paratill (TM) provided the highest productivity and lowest fuel cost when operated at the typical speeds in the Southeastern US (around the normal to fast) mostly likely due to the lower required draft. In summary, the minimum fuel usage for each implement occurred at a different ground speed however, productivity was maximized at the fast speed with data reported useful to practitioners managing these style tillage implements. Published by Elsevier B.V. C1 [Fulton, J. P.; McDonald, T. P.] Auburn Univ, Biosyst Engn Dept, Auburn, AL 36849 USA. [Kichler, C. M.] USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Raper, R. L.] USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Zech, W. C.] Auburn Univ, Dept Civil Engn, Auburn, AL 36849 USA. RP Fulton, JP (reprint author), Auburn Univ, Biosyst Engn Dept, 200 Tom E Corley Bldg, Auburn, AL 36849 USA. EM corey.kichler@ars.usda.gov; fultojp@auburn.edu; randy.raper@ars.usda.gov; mcdontp@auburn.edu; zechwes@auburn.edu FU USDA-ARS National Soil Dynamics Laboratory; Alabama Agricultural Experiment Station; CSREES-USDA; Alabama Wheat and Feed Grain Commodity group FX The authors would like to thank Tyrel Harbuck, Jerry Carrington and Eric Schwab who assisted with this research effort. A special thanks is extended to the reviewers who provided constructive feedback. We also appreciate the support provided by the USDA-ARS National Soil Dynamics Laboratory and the Alabama Agricultural Experiment Station. Partial funding for this project was provided through a special grant from CSREES-USDA entitled, "Precision Agriculture - Alabama" and the Alabama Wheat and Feed Grain Commodity group. NR 25 TC 10 Z9 10 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 J9 SOIL TILL RES JI Soil Tillage Res. PD JUN PY 2011 VL 113 IS 2 BP 105 EP 111 DI 10.1016/j.still.2011.03.002 PG 7 WC Soil Science SC Agriculture GA 767XQ UT WOS:000290892600005 ER PT J AU Asadauskas, S Perez, JM Erhan, SZ AF Asadauskas, Svajus Perez, Joseph M. Erhan, Sevim Z. TI Kinetics of Oxidative Degradation: Applicability of Time and Temperature Superposition SO TRIBOLOGY & LUBRICATION TECHNOLOGY LA English DT Article DE Vegetable Oils; Oleates; Triglycerides; Triolein; Unsaturation; Lubricants; Hydraulic Fluids; Biodegradable Oils; Polymerization; Gelation; Stability; Micro Oxidation; Kinetics AB New methodology is suggested to quantify the effect of temperature on oxidative degradation and compare test results obtained at different temperatures or evaluate changes in chemical mechanisms. Assumption of the pseudo-first-order kinetics to calculate activation energies in oxidative polymerization of uninhibited 100 percent oleic triglyceride under thin film oxidation provided only semiquantitative results from available oxypolymerization data at six temperatures from 100 degrees to 175 degrees C. As an alternative approach, Time and Temperature Superposition (TTS) was applied to analyze the data. Several TTS forms consistent with Arrhenius equation showed much greater accuracy in determining activation energies and induction periods. Different stages of oxidative degradation were identified after the data from different temperatures were combined into one kinetic map. Kinetic maps of alkalirefined high oleic sunflower oil, fully formulated synthetic oleate-based hydraulic fluid and additive-free super refined mineral oil showed their increasing resistance to oxypolymerization. Limitations of TTS were discussed. C1 [Asadauskas, Svajus; Perez, Joseph M.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA. [Erhan, Sevim Z.] USDA, NCAUR, Peoria, IL USA. RP Asadauskas, S (reprint author), Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA. RI Asadauskas, Svajus/A-6766-2010 OI Asadauskas, Svajus/0000-0001-9267-5280 NR 15 TC 1 Z9 1 U1 0 U2 3 PU SOC TRIBOLOGISTS & LUBRICATION ENGINEERS PI PARK RIDGE PA 840 BUSSE HIGHWAY, PARK RIDGE, IL 60068 USA SN 1545-858X J9 TRIBOL LUBR TECHNOL JI Tribol. Lubr. Technol. PD JUN PY 2011 VL 67 IS 6 BP 56 EP + PG 8 WC Engineering, Mechanical SC Engineering GA 766JT UT WOS:000290779100009 ER PT J AU Fan, ZS Casey, FXM Hakk, H Larsen, GL Khan, E AF Fan, Zhaosheng Casey, Francis X. M. Hakk, Heldur Larsen, Gerald L. Khan, Eakalak TI Sorption, Fate, and Mobility of Sulfonamides in Soils SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Batch experiments; Global optimization; Miscible-displacement; Solute transport; Sorption; Sulfamethazine ID VETERINARY ANTIMICROBIAL SULFATHIAZOLE; STOCHASTIC RANKING; LIQUID-CHROMATOGRAPHY; PARAMETER-ESTIMATION; CLAY-MINERALS; TRANSPORT; ANTIBIOTICS; METABOLITES; PHARMACEUTICALS; SULFAMETHAZINE AB Sulfonamides (SAs) are one of the broadly used antibiotics in domestic animal operations and have a notable potential of entering the environment through animal manure management practices. In this study, sulfamethazine (SMZ) was used as a prototype to study the sorption, fate, and transport of SAs in soil-water systems using batch and miscible-displacement experiments. Sulfamethazine was degraded to a polar metabolite (PM). The batch experiments indicated that the linear sorption partitioning coefficient (K (d) ) values for the PM ranged from 7.5 to 206.2 L kg(-1). Strong relationships between the sorption of PM and various soil fractions and organic matter were also observed. The miscible-displacement experiments showed that 33-70% of SMZ was degraded within 6 h during transport in the soil columns. Also, 69-99.7% of SMZ and PM were recovered in the effluents suggesting their high mobility. Also, the simultaneous degradation, sorption, and transport of SMZ and PM were described using a two-site chemical nonequilibrium fate and transport model, using the K (d) values obtained from the batch experiments. The parameters of this model were uniquely estimated using a global optimization strategy, the stochastic ranking evolutionary strategy. C1 [Fan, Zhaosheng] Univ Colorado, Dept Geol Sci, Boulder, CO 80309 USA. [Casey, Francis X. M.] N Dakota State Univ, Dept Soil Sci, Fargo, ND 58108 USA. [Hakk, Heldur; Larsen, Gerald L.] USDA ARS, Anim Metab & Agr Chem Res Unit, Biosci Res Lab, Fargo, ND 58108 USA. [Khan, Eakalak] N Dakota State Univ, Dept Civil Engn, Dept 2470, Fargo, ND 58108 USA. RP Fan, ZS (reprint author), Univ Colorado, Dept Geol Sci, Boulder, CO 80309 USA. EM Zhaosheng.Fan@Colorado.edu; francis.casey@ndsu.edu; Heldur.Hakk@ars.usda.gov; Gerald.Larsen@ars.usda.gov; eakalak.khan@ndsu.edu RI Casey, Francis/A-2135-2010 OI Casey, Francis/0000-0002-6035-7234 FU National Science Foundation [0730492] FX This research was based upon work supported by the National Science Foundation under Grant No. 0730492. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The authors also wish to express gratitude to Barbara K. Magelky and Colleen M. Pfaff for their contributions to the experimental work. NR 42 TC 20 Z9 21 U1 6 U2 58 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD JUN PY 2011 VL 218 IS 1-4 BP 49 EP 61 DI 10.1007/s11270-010-0623-6 PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA 765QT UT WOS:000290724400007 ER PT J AU Bodinier, M Denery, S Pineau, F Triballeau, S Leroy, M Tranquet, O Adel-Patient, K Moneret-Vautrin, D Bakan, B Marion, D Lelion, A Mothes, T Mameri, H Kasarda, D AF Bodinier, M. Denery, S. Pineau, F. Triballeau, S. Leroy, M. Tranquet, O. Adel-Patient, K. Moneret-Vautrin, D. Bakan, B. Marion, D. Lelion, A. Mothes, T. Mameri, H. Kasarda, D. TI Comparison of IgE-binding epitopes on wheat gliadins for patients with food allergy and experimentally sensitised mice SO ALLERGY LA English DT Meeting Abstract CT 30th Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI) CY JUN 11-15, 2011 CL Istanbul, TURKEY SP European Acad Allergy & Clin Immunol (EAACI) C1 [Bodinier, M.; Denery, S.; Pineau, F.; Triballeau, S.; Leroy, M.; Tranquet, O.; Bakan, B.; Marion, D.; Lelion, A.; Mameri, H.] INRA, Allergy Team, BIA UR1268, F-44026 Nantes, France. [Adel-Patient, K.] CEA Saclay, INRA, UR496, IAA, F-91191 Gif Sur Yvette, France. [Moneret-Vautrin, D.] Univ Hosp, Dept Internal Med Clin Immunol & Allergol, Nancy, France. [Mothes, T.] Univ Hosp, Inst Lab Med, Leipzig, Germany. [Mothes, T.] Univ Leipzig, Fac Med, D-04109 Leipzig, Germany. [Kasarda, D.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0105-4538 EI 1398-9995 J9 ALLERGY JI Allergy PD JUN PY 2011 VL 66 SU 94 SI SI MA 75 BP 36 EP 37 PG 2 WC Allergy; Immunology SC Allergy; Immunology GA 286IS UT WOS:000329462200076 ER PT J AU Maleki, S Cheng, H Dyer, S Kado, R Nesbit, J AF Maleki, S. Cheng, H. Dyer, S. Kado, R. Nesbit, J. TI The role of structure and chemical modification on the allergenicity of the major peanut allergens SO ALLERGY LA English DT Meeting Abstract CT 30th Congress of the European-Academy-of-Allergy-and-Clinical-Immunology (EAACI) CY JUN 11-15, 2011 CL Istanbul, TURKEY SP European Acad Allergy & Clin Immunol (EAACI) C1 [Maleki, S.; Cheng, H.; Nesbit, J.] USDA, New Orleans, LA USA. [Dyer, S.; Kado, R.] Tulane Hlth Sci Cener, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0105-4538 EI 1398-9995 J9 ALLERGY JI Allergy PD JUN PY 2011 VL 66 SU 94 SI SI MA 1008 BP 389 EP 389 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA 286IS UT WOS:000329462202191 ER PT J AU Taheri, WI Bever, JD AF Taheri, Wendy I. Bever, James D. TI Adaptation of Liquidambar styraciflua to coal tailings is mediated by arbuscular mycorrhizal fungi SO APPLIED SOIL ECOLOGY LA English DT Article DE Mycorrhizal; Fungi; Adaptation; Coal; Restoration; Remediation ID ANDROPOGON-VIRGINICUS L.; ALUMINUM RESISTANCE; DROUGHT STRESS; FLY-ASH; GROWTH; PLANTS; TOLERANCE; DIVERSITY; WHEAT; PRODUCTIVITY AB We performed a full factorial greenhouse experiment in order to determine if utilizing seedlings of Liquidambar styraciflua or communities of arbuscular mycorrhizal (AM) fungi, originating from coal mine conditions, could improve plant survivorship or accelerate revegetation of abandoned coal sites. Trees from the mine grew significantly more slowly than trees from natural areas. Both plant populations grew relatively better in their own soil type. Moreover, an AM symbiosis appeared to mediate local plant adaptation. Mine-soil adapted seedlings were more responsive to AM fungal colonization when grown in mine soil whereas the seedlings from natural areas were more responsive to AM fungal colonization when grown in Indiana's native low nutrient clay soil. AM fungal communities originating from the mined area showed significantly greater colonization levels in mine soil than in native soil, suggesting adaptation of AM fungi to mine-soil conditions. These results suggest that reclamation efforts could be improved by starting with plants and AM fungal communities which are already adapted to the target site. (C) 2011 Elsevier B.V. All rights reserved. C1 [Taheri, Wendy I.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Bever, James D.] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. RP Taheri, WI (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM wendy.taheri@ars.usda.gov; jbever@indiana.edu FU Indiana Dept of Natural Resources, Division of Coal Mine Restoration; National Science Foundation (NSF) [DEB-0919434] FX We would like to thank the Indiana Dept of Natural Resources, Division of Coal Mine Restoration for funding under the SMART Grant program, as well as the National Science Foundation (NSF grant DEB-0919434), and the Indiana Geological Survey for access to their database, and chemical analyses of Indiana coal. NR 29 TC 5 Z9 5 U1 3 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JUN PY 2011 VL 48 IS 2 BP 251 EP 255 DI 10.1016/j.apsoil.2011.03.012 PG 5 WC Soil Science SC Agriculture GA 781TC UT WOS:000291958400019 ER PT J AU Lee, LF Zhang, HM Heidari, M Lupiani, B Reddy, SM AF Lee, Lucy F. Zhang, Huanmin Heidari, Mohammad Lupiani, Blanca Reddy, Sanjay M. TI Evaluation of Factors Affecting Vaccine Efficacy of Recombinant Marek's Disease Virus Lacking the Meq Oncogene in Chickens SO AVIAN DISEASES LA English DT Article DE Marek's disease virus; recombinant MDV; Meq oncogene; vaccine parameters; vaccine efficacy ID BACTERIAL ARTIFICIAL CHROMOSOME; STRAIN CVI 988; MATERNAL ANTIBODIES; BROILER-CHICKENS; HERPESVIRUS; PROTECTION; CHALLENGE; GENE; MDV; NETHERLANDS AB We previously reported that deletion of the Meq gene from the oncogenic rMd5 virus rendered it apathogenic for chickens. Here we examined multiple factors affecting Marek's disease vaccine efficacy of this nonpathogenic recombinant Meq null rMd5 virus (rMd5 Delta Meq). These factors included host genetics (MHC haplotype), strain or dose of challenge virus, vaccine challenge intervals, and maternal antibody status of the vaccinated chicks. Studies on host genetics were carried out in five chicken lines comprising four different MHC B-haplotypes. Results showed that chicken lines tested were highly protected, with protective indexes of 100% (B*2/*15), 94% (B*2/*2), 87% (B*19/*19), and 83% (B*21/*21). At a challenge dose above 8000 plaque-forming units, differences in protection were observed between the two highly virulent strains examined (648A and 686). The interval between vaccination and challenge indicated a protective efficacy from 0 to 2 days varied greatly (12%-82%) after challenge with vv+686, the most virulent virus. Less variation and significant protection began at 3 days post vaccination and reached a maximum at 5 days post vaccination with about 80%-100% protection. Taken together, our results indicate that the factors examined in this study are important for vaccine efficacy and need to be considered in comparative evaluations of vaccines. C1 [Lee, Lucy F.; Zhang, Huanmin; Heidari, Mohammad] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Lupiani, Blanca; Reddy, Sanjay M.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathol, College Stn, TX 77843 USA. RP Lee, LF (reprint author), ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. EM leelu@msu.edu NR 42 TC 4 Z9 4 U1 0 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2011 VL 55 IS 2 BP 172 EP 179 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 786ZZ UT WOS:000292347700002 PM 21793430 ER PT J AU Gast, RK Guraya, R Guard, J Holt, PS AF Gast, Richard K. Guraya, Rupa Guard, Jean Holt, Peter S. TI The Relationship Between the Numbers of Salmonella Enteritidis, Salmonella Heidelberg, or Salmonella Hadar Colonizing Reproductive Tissues of Experimentally Infected Laying Hens and Deposition Inside Eggs SO AVIAN DISEASES LA English DT Article DE Salmonella Enteritidis; Salmonella Heidelberg; Salmonella Hadar; chickens; egg contamination; ovary; oviduct ID ENTERICA SEROVAR ENTERITIDIS; LIPOPOLYSACCHARIDE O-CHAIN; UNITED-STATES; PHAGE TYPE-4; SEROTYPE ENTERITIDIS; HORIZONTAL TRANSMISSION; CONTAMINATION; COLONIZATION; STRAINS; CHICKENS AB Contamination of eggs by Salmonella Enteritidis has been a prominent cause of human illness for several decades and is the focus of a recently implemented national regulatory, plan for egg-producing flocks in the United States. Salmonella Heidelberg has also been identified as an egg-transmitted pathogen. The deposition of Salmonella strains inside eggs is a consequence of reproductive tract colonization in infected laying hens, but prior research has not determined the relationship between the numbers of Salmonella that colonize reproductive organs and the associated frequency of egg contamination. In the present study, groups of laying hens in two trials were experimentally infected with large oral doses of strains of Salmonella Enteriridis (phage type 13a), Salmonella Heidelberg, or Salmonella Hadar. Reproductive tissues of selected hens were cultured to detect and enumerate Salmonella at 5 days postinoculation, and the interior contents of eggs laid between 6 and 25 days postinoculation were tested for contamination. Significantly more internally contaminated eggs were laid by hens infected with Salmonella Enteritidis (3.58%) than with strains of either Salmonella Heidelberg (0.47%) or Salmonella Hadar (0%). However, no significant differences were observed between Salmonella strains in either isolation frequency, or the number of colony-forming units (CPU) isolated from ovaries or oviducts. Salmonella isolation frequencies ranged from 20.8% to 41.7% for ovaries and from 8.3% to 33.3% for oviducts. Mean Salmonella colonization levels ranged from 0.10 to 0.51 log CFU/g for ovaries and from 0.25 to 0.46 log CFU/g for oviducts. Although parallel rank-orders were observed for Salmonella enumeration (in both ovaries and oviducts) and egg contamination frequency, a statistically, significant relationship could not be established between these two parameters of infection. C1 [Gast, Richard K.; Guraya, Rupa; Guard, Jean; Holt, Peter S.] ARS, USDA, Egg Safety & Qual Res Unit, Russell Res Ctr, Athens, GA 30605 USA. RP Gast, RK (reprint author), ARS, USDA, Egg Safety & Qual Res Unit, Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM richard.gast@ars.usda.gov NR 47 TC 17 Z9 17 U1 2 U2 15 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2011 VL 55 IS 2 BP 243 EP 247 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 786ZZ UT WOS:000292347700012 PM 21793440 ER PT J AU Mesonero, A Suarez, DL van Santen, E Tang, DC Toro, H AF Mesonero, Alexander Suarez, David L. van Santen, Edzard Tang, De-chu C. Toro, Haroldo TI Avian Influenza In Ovo Vaccination with Replication Defective Recombinant Adenovirus in Chickens: Vaccine Potency, Antibody Persistence, and Maternal Antibody Transfer SO AVIAN DISEASES LA English DT Article DE avian influenza virus; recombinant vaccine; adenovirus; chickens ID VIRUS; HEMAGGLUTININ; PROTECTION AB Protective immunity against avian influenza (AI) can be elicited in chickens in a single-dose regimen by in ova vaccination with a replication-competent adenovirus (RCA)-free human adenovirus serotype 5 (Ad)-vector encoding the AI virus (AIV) hemagglutinin (HA). We evaluated vaccine potency, antibody persistence, transfer of maternal antibodies (MtAb), and interference between MtAb and active in ovo or mucosal immunization with RCA-free recombinant Ad expressing a codon-optimized AIV H5 HA gene from A/turkey/WI/68 (AdTW68.H5(ck)). Vaccine coverage and intrapotency test repeatability were based on anti-H5 hemagglutination inhibition (HI) antibody levels detected in in ovo vaccinated chickens. Even though egg inoculation of each replicate was performed by individuals with varying expertise and with different vaccine batches, the average vaccine coverage of three replicates was 85%. The intrapotency test repeatability, which considers both positive as well as negative values, varied between 0.69 and 0.71, indicating effective vaccination. Highly pathogenic (HP) ATV challenge of chicken groups vaccinated with increasing vaccine doses showed similar to 90% protection in chickens receiving >= 10(8) ifu (infectious units)/bird. The protective dose 50% (PD(50)) was determined to be 10(6.5) ifu. Even vaccinated chickens that did not develop detectable antibody levels were effectively protected against HP AIV challenge. This result is consistent with previous findings of Ad-vector eliciting T lymphocyte responses. Higher vaccine doses significantly reduced viral shedding as determined by AIV RNA concentration in oropharyngeal swabs. Assessment of antibody persistence showed that antibody levels of in ova immunized chickens continued to increase until 12 wk and started to decline after 18 wk of age. Intramuscular (IM) booster vaccination with the same vaccine at 16 wk of age significantly increased the antibody responses in breeder hens, and these responses were maintained at high levels throughout the experimental period (34 wk of age). AdTW68.H5(ch)-immunized breeder hens effectively transferred MtAb to progeny chickens. The level of MtAb in the progenies was consistent with the levels detected in the breeders, i.e., intramuscularly boosted breeders transferred higher concentrations of antibodies to the offspring. Maternal antibodies declined with time in the progenies and achieved marginal levels by 34 days of age. Chickens with high maternal antibody levels that were vaccinated either in ova or via mucosal routes (ocular or spray) did not seroconvert. In contrast, chickens without MtAb successfully developed specific antibody levels after either in ova or mucosal vaccination. These results indicate that high levels of MtAb interfered with active Advectored vaccination. C1 [Mesonero, Alexander; Toro, Haroldo] Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA. [Suarez, David L.] USDA, SE Poultry Res Lab, Athens, GA 30605 USA. [van Santen, Edzard] Auburn Univ, Dept Agron & Soils, Auburn, AL 36849 USA. [Tang, De-chu C.] Vaxin Inc, Birmingham, AL 35203 USA. RP Toro, H (reprint author), Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA. EM torohar@auburn.edu FU USDA; National Institutes of Health FX The authors wish to thank Cassandra Breedlove, Frederik van Ginkel, Lisa Parsons, and Rodrigo Gallardo at Auburn University, Suzanne DeBlois at SEPRL, Jianfeng Zhang, Tsungwei Feng, and Zhongkai Shi at Vaxin Inc. for technical assistance during this work. This work was funded by USDA Avian Influenza Coordinated Agricultural Project and the Small Business Research Funding Opportunities, National Institutes of Health. NR 17 TC 8 Z9 8 U1 1 U2 9 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2011 VL 55 IS 2 BP 285 EP 292 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 786ZZ UT WOS:000292347700019 PM 21793447 ER PT J AU Hunt, HD Dunn, JR AF Hunt, Henry D. Dunn, John R. TI Serial Transfer of a Transplantable Tumor: Implications for Marek's Vaccine Mechanisms SO AVIAN DISEASES LA English DT Article DE Marek's disease; virus; vaccination; virulence; transplantable tumor ID STRAIN CVI 988; DISEASE VIRUS; GROWTH-CHARACTERISTICS; CHICKEN LINES; INBRED LINES; FIELD TRIALS; CELL-LINES; REPERTOIRE; HERPESVIRUS; PROTECTION AB The mechanism of Marek's disease (MD) vaccination to prevent the lymphoproliferative disease in chickens is not well understood. It is generally recognized that vaccination prevents disease, including the induction of T-cell tumors, but it does not prevent the pathogenic virus from infecting and replicating in the vaccinated host, nor does it prevent bird to bird spread of the oncogenic virus. The stage at which the vaccinated immune system intervenes in the process from infection to the induction of tumors remains obscure. Using a transplantable tumor induced by the Md5 strain of MD virus (MDV), we show that CVI988 vaccination does not prevent the induction of transplantable tumors in the 15I(5) X 7(1) chicken line. A monoclonal tumor with a V beta 1 T-cell receptor spectrarype of 207 base pairs was used to follow the transplantable tumor in serial passages in vivo. This transplantable tumor could be passed in vaccinated birds. The length of time between vaccination and challenge (5 to 12 days) had little or no influence on the ability to transfer the tumor. There was variability in the manifestation of the disease produced by the transplanted tumor. Some chickens presented as normal but were still capable of transmitting the transplanted tumor to newly vaccinated recipients via their blood. This indicates that some chickens can control, but not eliminate, the tumor. The variables inducing health or disease in the challenged chickens remain obscure, but environmental or other factors likely depress the immune system allowing the tumor to overwhelm the immune system. C1 [Hunt, Henry D.; Dunn, John R.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Hunt, HD (reprint author), ARS, USDA, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM Henry.Hunt@ars.usda.gov NR 35 TC 2 Z9 2 U1 0 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2011 VL 55 IS 2 BP 293 EP 301 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 786ZZ UT WOS:000292347700020 PM 21793448 ER PT J AU Shafer, SR Walthall, CL Franzluebbers, AJ Scholten, M Meijs, J Clark, H Reisinger, A Yagi, K Roel, A Slattery, B Campbell, ID McConkey, BG Angers, DA Soussana, JF Richard, G AF Shafer, Steven R. Walthall, Charles L. Franzluebbers, Alan J. Scholten, Martin Meijs, Jac Clark, Harry Reisinger, Andy Yagi, Kazuyuki Roel, Alvaro Slattery, Bill Campbell, Ian D. McConkey, Brian G. Angers, Denis A. Soussana, Jean-Francois Richard, Guy TI Emergence of the Global Research Alliance on Agricultural Greenhouse Gases SO CARBON MANAGEMENT LA English DT Editorial Material ID SOIL C1 [Shafer, Steven R.; Walthall, Charles L.] ARS, USDA, Beltsville, MD 20705 USA. [Franzluebbers, Alan J.] ARS, USDA, Watkinsville, GA 30677 USA. [Scholten, Martin; Meijs, Jac] Wageningen Univ, NL-6700 AP Wageningen, Netherlands. [Clark, Harry; Reisinger, Andy] New Zealand Agr Greenhouse Gas Res Ctr, Palmerston North 4442, New Zealand. [Yagi, Kazuyuki] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan. [Roel, Alvaro] Natl Inst Agr Res, Treinta 33000, Uruguay. [Roel, Alvaro] Natl Inst Agr Res, Tres 33000, Uruguay. [Slattery, Bill] Dept Climate Change & Energy Efficiency, Canberra, ACT 2600, Australia. [Campbell, Ian D.] Agr & Agri Food Canada, Ottawa, ON, Canada. [McConkey, Brian G.] Agr & Agri Food Canada, Swift Current, SK S9H 3X2, Canada. [Angers, Denis A.] Agr & Agri Food Canada, Ste Foy, PQ G1V 2J3, Canada. [Soussana, Jean-Francois] INRA, F-75338 Paris 07, France. [Richard, Guy] Ctr Rech Orleans, INRA, F-45075 Orleans 2, France. RP Franzluebbers, AJ (reprint author), ARS, USDA, 1420 Expt Stn Rd, Watkinsville, GA 30677 USA. EM alan.franzluebbers@ars.usda.gov RI Soussana, Jean-Francois/P-2094-2016; Soussana, Jean-Francois/E-2543-2012; Reisinger, Andy/E-1201-2016 OI Soussana, Jean-Francois/0000-0002-1932-6583; Campbell, Ian/0000-0002-9639-1995; Reisinger, Andy/0000-0002-6631-7188 NR 15 TC 9 Z9 9 U1 2 U2 7 PU FUTURE SCI LTD PI LONDON PA UNITED HOUSE, 2 ALBERT PL, LONDON, N3 1QB, ENGLAND SN 1758-3004 J9 CARBON MANAG JI Carbon Manag. PD JUN PY 2011 VL 2 IS 3 BP 209 EP 214 DI 10.4155/CMT.11.26 PG 6 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA V27ZE UT WOS:000208650300001 ER PT J AU Lippke, B Oneil, E Harrison, R Skog, K Gustavsson, L Sathre, R AF Lippke, Bruce Oneil, Elaine Harrison, Rob Skog, Kenneth Gustavsson, Leif Sathre, Roger TI Life cycle impacts of forest management and wood utilization on carbon mitigation: knowns and unknowns SO CARBON MANAGEMENT LA English DT Review AB This review on research on life cycle carbon accounting examines the complexities in accounting for carbon emissions given the many different ways that wood is used. Recent objectives to increase the use of renewable fuels have raised policy questions, with respect to the sustainability of managing our forests as well as the impacts of how best to use wood from our forests. There has been general support for the benefits of sustainably managing forests for carbon mitigation as expressed by the Intergovernmental Panel on Climate Change in 2007. However, there are many integrated carbon pools involved, which have led to conflicting implications for best practices and policy. In particular, sustainable management of forests for products produces substantially different impacts than a focus on a single stand or on specific carbon pools with each contributing to different policy implications. In this article, we review many recent research findings on carbon impacts across all stages of processing from cradle-to-grave, based on life cycle accounting, which is necessary to understand the carbon interactions across many different carbon pools. The focus is on where findings are robust and where uncertainties may be large enough to question key assumptions that impact carbon in the forest and its many uses. Many opportunities for reducing carbon emissions are identified along with unintended consequences of proposed policies. C1 [Lippke, Bruce] Univ Washington, Coll Environm, Seattle, WA 98195 USA. [Oneil, Elaine] CORRIM Inc Consortium Res Renewable Ind Mat, Seattle, WA 98195 USA. [Harrison, Rob] Univ Washington, Coll Environm, Seattle, WA 98195 USA. [Skog, Kenneth] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Gustavsson, Leif] Linneaus Univ, Kalmar, Sweden. [Gustavsson, Leif; Sathre, Roger] Mid Sweden Univ, Harnosand, Sweden. RP Lippke, B (reprint author), Univ Washington, Coll Environm, Box 352100, Seattle, WA 98195 USA. EM blippke@u.washington.edu FU CORRIM FX While our review goes far beyond CORRIM's research, we acknowledge the support of CORRIM's member institutions and contributing researchers, donors and agencies, in particular the US Forest Service. Through their support we had access to never-before accumulated life cycle data and international contacts that enriched our understanding of the complex interactions between many carbon pools. We thank the Swedish Ministry of Industry for its leadership in understanding the carbon impacts of different buildings and support for international collaboration. We thank the Society of Wood Science and Technology for their willingness to review and publish special issues bringing together much of the life cycle information on sustainable forest management and wood utilization. Any opinions, findings, conclusions or recommendations as expressed in this article are however those of the authors and do not necessarily reflect the views of the contributing entities. NR 80 TC 86 Z9 88 U1 1 U2 40 PU FUTURE SCI LTD PI LONDON PA UNITED HOUSE, 2 ALBERT PL, LONDON, N3 1QB, ENGLAND SN 1758-3004 J9 CARBON MANAG JI Carbon Manag. PD JUN PY 2011 VL 2 IS 3 BP 303 EP 333 DI 10.4155/CMT.11.24 PG 31 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA V27ZE UT WOS:000208650300015 ER PT J AU Coleman, TW Seybold, SJ AF Coleman, Tom W. Seybold, Steven J. TI COLLECTION HISTORY AND COMPARISON OF THE INTERACTIONS OF THE GOLDSPOTTED OAK BORER, AGRILUS AUROGUTTATUS SCHAEFFER (COLEOPTERA: BUPRESTIDAE), WITH HOST OAKS IN SOUTHERN CALIFORNIA AND SOUTHEASTERN ARIZONA, U.S.A. SO COLEOPTERISTS BULLETIN LA English DT Article DE Agrilus coxalis; Atanycolus simplex; Calosota elongata; firewood; intracontinental invasive species; oak mortality ID ENEMY RELEASE HYPOTHESIS; UNITED-STATES; MEXICO; FIREWOOD; INVASION; RECORDS; OREGON; CANADA; NORTH; BARK AB An invasive population of the goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), is colonizing and killing three species of oaks in San Diego Co., California. However, the interactions of A. (novo:lams with oaks in its native range in southeastern Arizona have not been recorded. We present a complete inventory of the North and Central American collection records of A. auroguttatus and Agrilus coxalis Waterhouse from the literature and from a survey of the holdings of 27 museum and personal collections. We also discuss the relationship between this collection history and the behavior of A. auroguttatus as an intracontinental invasive species. Surveys of native populations of A. auroguttatus in oak forest stands from four mountain ranges in southeastern Arizona revealed injury patterns on Emory oak, Quercus emoryi Torrey, and silverleaf oak, Quercus hypoleucoides A. Camus, similar to those observed on other "red" oaks in California. No damage was observed on "white" oaks in Arizona, and observed only rarely on a white oak, Quercus engelmannii Greene, in California. In Arizona, adult emergence was confirmed from bark removed from Q. emoryi, representing the first developmental record of A. auroguttatus from a native host. Late instars of Agrilus sp. were also recovered from Q. hypoleucoides, but they were not reared to the adult stage for species identification. Nonetheless, our observations of damage and the presence of larvae in the same configuration and location in the outer bark as we would expect for A. auroguttatus suggest that Q. hypoleucoides is also likely a host. Two hymenopteran parasitoids, Calosota elongata Gibson (Eupelmidae) and Atanycolus simplex Cresson (Braconidae), and two likely coleopteran predators (Trogossitidae and Elateridae) emerged from, or were collected in southeastern Arizona from, Q. emoryi bark infested with A. auroguttatus. Based on the museum survey results, the morphological similarity of individuals from the California and Arizona populations, the spatial dynamics of the pattern of infestation in California, the geographic isolation of hosts in California from native populations of the beetle, and the proximity of San Diego Co. to southeastern Arizona, we hypothesize that A. auroguttatus was introduced to California from Arizona or less likely from the Mexican states of Baja California, Chihuahua, or Sonora, and that the introduction most likely occurred on oak firewood. Further, we hypothesize that the oak mortality in southern California is occurring from this intracontinental invasive species because the beetle is filling a vacant niche by colonizing and developing in non-coevolved trees with low host resistance in the absence of a diverse and coevolved insect natural enemy complex. C1 [Coleman, Tom W.] US Forest Serv, USDA, San Bernardino, CA 92408 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95616 USA. RP Coleman, TW (reprint author), US Forest Serv, USDA, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. EM twcoleman@fs.fed.us; sjseybold@gmail.com FU USDA Forest Service; Pacific Southwest Research Station; Invasive Species Program; Forest Health Protection, Region 5; Forest Health Monitoring Program [WC-DM-09-01] FX The authors want to thank Charles L. Bellamy (Plant Pest Diagnostic Laboratory, California Department of Food and Agriculture), Henry A. Hespenheide (Department of Ecology and Evolutionary Biology, University of California-Los Angeles), and Richard L. Westcott (Oregon Department of Agriculture), who generously shared their unpublished collection data. HAH and RLW also provided valuable comments on earlier versions of the manuscript. We also thank Michael Sharkey (Department of Entomology, University of Kentucky), Gary A. P. Gibson (Canadian National Collection of Insects, Agriculture and Agri-Food Canada), Joel McMillin and Bobbe Fitzgibbon (USDA Forest Service-Forest Health Protection, Region 3), Andreana Cipollone, Zachary Heath, Andi Koonce (deceased), Meghan Woods, and Paul Zambino (USDA Forest Service-Forest Health Protection, Region 5), Andrew D. Graves (Department of Plant Pathology, University of California-Davis), Steve Heydon and Stacy Hishinuma (Department of Entomology, University of California- Davis), Robert Rabaglia (USDA Forest Service-Forest Health Protection, Washington Office), and the staffs of the Cleveland and Coronado National Forests for their support of and assistance with this work. We thank James Boone (Field Museum of Natural History), Shawn Clark (Monte L. Bean Life Science Museum, Brigham Young University), Lee Herman (American Museum of Natural History), Shepherd Myers (Bernice P. Bishop Museum), Philip Perkins (Museum of Comparative Zoology, Harvard University), Natalia Vandenberg (National Museum of Natural History), and Michael Thomas (Florida State Collection of Arthropods) for generously taking the time to search for and report specimen records from their respective collections (Table 1). Specimens of A. auroguttatus reared from Q. emoryi from AZ were deposited in the CAS, specimens of C. elongata reared from A. auroguttatus-infested bark from AZ were deposited in the CNCI and UCDC, and specimens of A. simplex reared from A. auroguttatus-infested bark or logs were deposited in the UCDC (CA specimens) and the University of Kentucky Insect Collection (HIC) (AZ specimens). Initial critical funding for this work was provided primarily by the USDA Forest Service, Pacific Southwest Research Station, Invasive Species Program, Forest Health Protection, Region 5, and by the Forest Health Monitoring Program (Project # WC-DM-09-01). NR 53 TC 26 Z9 26 U1 2 U2 22 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2011 VL 65 IS 2 BP 93 EP 108 PG 16 WC Entomology SC Entomology GA 783IL UT WOS:000292074600001 ER PT J AU Lingafelter, SW Nearns, EH AF Lingafelter, Steven W. Nearns, Eugenio H. TI NEW SYNONYMY IN CUBAN TILLOCLYTUS BATES (COLEOPTERA: CERAMBYCIDAE: ANAGLYPTINI) SO COLEOPTERISTS BULLETIN LA English DT Editorial Material C1 [Lingafelter, Steven W.] ARS, Systemat Entomol Lab, Inst Plant Sci, USDA,Natl Museum Nat Hist, Washington, DC 20013 USA. [Nearns, Eugenio H.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. RP Lingafelter, SW (reprint author), ARS, Systemat Entomol Lab, Inst Plant Sci, USDA,Natl Museum Nat Hist, Washington, DC 20013 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2011 VL 65 IS 2 BP 153 EP 153 PG 1 WC Entomology SC Entomology GA 783IL UT WOS:000292074600011 ER PT J AU Negron, JF Schaupp, WC Pederson, L AF Negron, Jose F. Schaupp, Willis C., Jr. Pederson, Lee TI FLIGHT PERIODICITY OF THE DOUGLAS-FIR BEETLE, DENDROCTONUS PSEUDOTSUGAE HOPKINS (COLEOPTERA: CURCULIONIDAE: SCOLYTINAE) IN COLORADO, U.S.A. SO COLEOPTERISTS BULLETIN LA English DT Editorial Material ID CLIMATE-CHANGE; MORTALITY C1 [Negron, Jose F.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Schaupp, Willis C., Jr.] US Forest Serv, USDA, Forest Hlth Protect Rapid City Serv Ctr, Rapid City, SD 57702 USA. [Pederson, Lee] US Forest Serv, USDA, Forest Hlth Protect Coeur dAlene Field Off, Coeur Dalene, ID 83815 USA. RP Negron, JF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 240 W Prospect, Ft Collins, CO 80526 USA. EM jnegron@fs.fed.us NR 12 TC 1 Z9 1 U1 0 U2 7 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2011 VL 65 IS 2 BP 182 EP 184 PG 3 WC Entomology SC Entomology GA 783IL UT WOS:000292074600016 ER PT J AU Oppert, B Elpidina, EN Toutges, M Mazumdar-Leighton, S AF Oppert, Brenda Elpidina, Elena N. Toutges, Michelle Mazumdar-Leighton, Sue TI Microarray analysis reveals strategies of Tribolium castaneum larvae to compensate for cysteine and serine protease inhibitors, (vol 5, pg 280, 2010) SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS LA English DT Correction C1 [Oppert, Brenda; Toutges, Michelle] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Elpidina, Elena N.] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119991, Russia. [Mazumdar-Leighton, Sue] Univ Delhi, Dept Bot, Delhi 110007, India. RP Oppert, B (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM bso@ksu.edu NR 1 TC 0 Z9 0 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1744-117X J9 COMP BIOCHEM PHYS D JI Comp. Biochem. Physiol. D-Genomics Proteomics PD JUN PY 2011 VL 6 IS 2 BP 235 EP 235 DI 10.1016/j.cbd.2011.02.001 PG 1 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 781GP UT WOS:000291918900016 ER PT J AU Dubey, A Farmer, A Schlueter, J Cannon, SB Abernathy, B Tuteja, R Woodward, J Shah, T Mulasmanovic, B Kudapa, H Raju, NL Gothalwal, R Pande, S Xiao, YL Town, CD Singh, NK May, GD Jackson, S Varshney, RK AF Dubey, Anuja Farmer, Andrew Schlueter, Jessica Cannon, Steven B. Abernathy, Brian Tuteja, Reetu Woodward, Jimmy Shah, Trushar Mulasmanovic, Benjamin Kudapa, Himabindu Raju, Nikku L. Gothalwal, Ragini Pande, Suresh Xiao, Yongli Town, Chris D. Singh, Nagendra K. May, Gregory D. Jackson, Scott Varshney, Rajeev K. TI Defining the Transcriptome Assembly and Its Use for Genome Dynamics and Transcriptome Profiling Studies in Pigeonpea (Cajanus cajan L.) SO DNA RESEARCH LA English DT Article DE Cajanus cajan L.; next generation sequencing; transcriptome assembly; molecular markers and gene discovery ID FINGER PROTEIN ZAT12; SSR-MARKERS; MICROSATELLITE MARKERS; EST RESOURCE; EXPRESSION; SEQUENCE; ARABIDOPSIS; DISCOVERY; ALIGNMENT; STRESSES AB This study reports generation of large-scale genomic resources for pigeonpea, a so-called 'orphan crop species' of the semi-arid tropic regions. FLX/454 sequencing carried out on a normalized cDNA pool prepared from 31 tissues produced 494 353 short transcript reads (STRs). Cluster analysis of these STRs, together with 10 817 Sanger ESTs, resulted in a pigeonpea trancriptome assembly (CcTA) comprising of 127 754 tentative unique sequences (TUSs). Functional analysis of these TUSs highlights several active pathways and processes in the sampled tissues. Comparison of the CcTA with the soybean genome showed similarity to 10 857 and 16 367 soybean gene models (depending on alignment methods). Additionally, Illumina 1G sequencing was performed on Fusarium wilt (FW)- and sterility mosaic disease (SMD)-challenged root tissues of 10 resistant and susceptible genotypes. More than 160 million sequence tags were used to identify FW- and SMD-responsive genes. Sequence analysis of CcTA and the Illumina tags identified a large new set of markers for use in genetics and breeding, including 8137 simple sequence repeats, 12 141 single-nucleotide polymorphisms and 5845 intron-spanning regions. Genomic resources developed in this study should be useful for basic and applied research, not only for pigeonpea improvement but also for other related, agronomically important legumes. C1 [Dubey, Anuja; Tuteja, Reetu; Shah, Trushar; Kudapa, Himabindu; Raju, Nikku L.; Pande, Suresh; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, CEG, Patancheru 502324, Greater Hyderab, India. [Dubey, Anuja; Gothalwal, Ragini] Barkatullah Univ, Bhopal 462026, India. [Farmer, Andrew; Woodward, Jimmy; May, Gregory D.] NCGR, Santa Fe, NM 87505 USA. [Schlueter, Jessica] Univ N Carolina, Charlotte, NC 28223 USA. [Cannon, Steven B.; Mulasmanovic, Benjamin] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Abernathy, Brian; Jackson, Scott] Purdue Univ, W Lafayette, IN 47907 USA. [Xiao, Yongli; Town, Chris D.] JCVI, Rockville, MD 20850 USA. [Singh, Nagendra K.] Natl Res Ctr Plant Biotechnol, New Delhi 110012, India. [Varshney, Rajeev K.] CIMMYT, CGIAR Generat Challenge Programme GCP, Mexico City 06600, DF, Mexico. RP Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, CEG, Bldg 300, Patancheru 502324, Greater Hyderab, India. EM r.k.varshney@cgiar.org RI Varshney, Rajeev/C-5295-2014 OI Varshney, Rajeev/0000-0002-4562-9131 FU CGIAR Generation Challenge Programme, Mexico; Pigeonpea Genomics Initiative (PGI) of Indian Council of Agricultural Research (ICAR) of Government of India FX This study was supported financially by SP2 Leader Discretionary Grant of CGIAR Generation Challenge Programme, Mexico; and Pigeonpea Genomics Initiative (PGI) of Indian Council of Agricultural Research (ICAR) of Government of India. NR 41 TC 48 Z9 48 U1 0 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1340-2838 J9 DNA RES JI DNA Res. PD JUN PY 2011 VL 18 IS 3 BP 153 EP 164 DI 10.1093/dnares/dsr007 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA 783GV UT WOS:000292070400002 PM 21565938 ER PT J AU Jensen, NR Webster, CR Witt, JC Grant, JB AF Jensen, N. R. Webster, C. R. Witt, J. C. Grant, J. B. TI Ungulate winter habitat selection as a driver of herbaceous-layer heterogeneity in northern temperate forests SO ECOSPHERE LA English DT Article DE Acer saccharum; biodiversity; biogeochemical hotspots; forest ecology; Odocoileus virginianus; ordination; plant community structure; Tsuga canadensis; white-tailed deer AB Concentrated seasonal habitat use by ungulates may amplify their influence on ecosystem processes. For example, white-tailed deer (Odocoileus virginianus) depend on forests of coniferous trees, such as Tsuga canadensis (L.) Carr. (eastern hemlock), for winter cover and forage in the northern portion of their range. We hypothesized that winter habitat use influences summer herbaceous-layer plant communities through cascading indirect effects resulting from the deposition of nitrogenous wastes and browsing of woody plants. Deer use was monitored from 2006-2008 via fecal pellet group counts in 15 T. canadensis stands across the western Upper Peninsula of Michigan. Detailed vegetation and soil sampling was performed during the summer of 2008. Estimates derived from winter inputs of fecal pellets, suggest that overwintering white-tailed deer deposit 3.3 +/- 0.8 kg N ha(-1) yr(-1). Results from nonmetric multidimensional scaling ordinations and permutational ANOVAs of distance to group centroids of ground-layer vegetation and soil attributes indicated that high levels of winter deer-use were significantly associated with greater heterogeneity in ground-layer community composition and relative soil nutrient variability within and between stands. Our results highlight potentially important feedbacks between habitat selection by ungulates, site productivity, and plant community structure in forest ecosystems. C1 [Jensen, N. R.; Webster, C. R.; Witt, J. C.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Ecosyst Sci Ctr, Houghton, MI 49931 USA. [Grant, J. B.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. RP Jensen, NR (reprint author), US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. EM cwebster@mtu.edu FU McIntier -Stennis Cooperative Forestry Program; Ecosystem Science Center at Michigan Technological University; Wildlife Division of the Michigan Department of Natural Resources FX We would like to thank the Michigan Department of Natural Resources, Ottawa National Forest, Michigan Technological University, and the Gratiot Lake Conservancy for logistical support and access to study sites. We would like to thank the following individuals for field and/or laboratory assistance: Jen Boettger, Jennifer Eikenberry, Steve Miceli, Justin Link, Andy Quinn, Chao Wang, and Aaron Wuori. Helpful comments on the design of this study and earlier drafts of this manuscript were provided by Joseph Bump, Andy Burton, Casey Huckins, Marty Jurgensen, and Erik Lilleskov. Support for this project was provided by the McIntier -Stennis Cooperative Forestry Program, Ecosystem Science Center at Michigan Technological University, and Wildlife Division of the Michigan Department of Natural Resources. NR 71 TC 7 Z9 7 U1 3 U2 14 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2011 VL 2 IS 6 AR UNSP art67 DI 10.1890/ES10-00189.1 PG 16 WC Ecology SC Environmental Sciences & Ecology GA V30IW UT WOS:000208810700003 ER PT J AU Kim, SP Kang, MY Choi, YH Kim, JH Nam, SH Friedman, M AF Kim, Sung Phil Kang, Mi Young Choi, Yong Hee Kim, Jae Ho Nam, Seok Hyun Friedman, Mendel TI Mechanism of Hericium erinaceus (Yamabushitake) mushroom-induced apoptosis of U937 human monocytic leukemia cells SO FOOD & FUNCTION LA English DT Article ID STOMACH-CANCER CELLS; RICE BRAN EXTRACTS; NUTRITIONAL QUALITY; NITRIC-OXIDE; HL-60 CELLS; DEATH; LIVER; GROWTH; ASSAYS; GLYCOALKALOIDS AB Phytochemicals in some foods are a potential source of bioactive safe compounds for cancer chemoprevention and suppression of tumor initiation, promotion, and metastasis. In the present study, we evaluated hot water (HWE), microwaved 50% ethanol (MWE), acidic (ACE), and alkaline (AKE) extracts of the fruitbody (sporocarp) of Hericium erinaceus (Yamabushitake, Lion's Mane) mushrooms for their ability to induce apoptosis (programmed cell death) in U937 human monocytic leukemia cells. Cell culture, cell viability, cytotoxicity, flow cytometry, chromosomal DNA integrity, mitochondrial membrane potential, expression of pro-and anti-apoptotic proteins, and activation and inhibition of caspase assays were carried out to help define the mechanism of observed apoptosis. The aqueous and aqueous/ethanolic extracts were active in all assays, whereas the acidic and alkaline extracts with the similar proximate compositions were both inactive. The results of the bioassays with the active extracts are consistent with an apoptosis mechanism governing suppression of the cell proliferation pathway that involves activation of mitochondria-mediated caspase-3 and caspase-9 but not caspase-8. Proximate analysis of the freeze-dried mushroom powder showed that it contains high amounts of proteins, carbohydrates, and minerals. The results indicate that H. erinaceus mushrooms may have therapeutic potential against human leukemia. C1 [Kim, Sung Phil; Kim, Jae Ho] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea. [Kang, Mi Young] Kyungpook Natl Univ, Dept Food Sci & Nutr, Taegu 702701, South Korea. [Choi, Yong Hee] Kyungpook Natl Univ, Dept Food Sci & Technol, Taegu 702701, South Korea. [Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Kim, SP (reprint author), Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea. EM shnam@ajou.ac.kr; Mendel.Friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 FU Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries [107082-03-1-HD110] FX This work was supported by a grant (No. 107082-03-1-HD110) from Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries. We thank Carol E. Levin for assistance with the preparation of the manuscript. NR 39 TC 18 Z9 19 U1 0 U2 10 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 2042-6496 J9 FOOD FUNCT JI Food Funct. PD JUN PY 2011 VL 2 IS 6 BP 348 EP 356 DI 10.1039/c1fo10030k PG 9 WC Biochemistry & Molecular Biology; Food Science & Technology SC Biochemistry & Molecular Biology; Food Science & Technology GA 782FC UT WOS:000291992800007 PM 21779573 ER PT J AU York, P Evangelista, P Kumar, S Graham, J Flather, C Stohlgren, T AF York, Patricia Evangelista, Paul Kumar, Sunil Graham, James Flather, Curtis Stohlgren, Thomas TI A habitat overlap analysis derived from Maxent for Tamarisk and the South-western Willow Flycatcher SO FRONTIERS OF EARTH SCIENCE LA English DT Article DE Niche modeling; species interactions; Tamarisk; South-western Willow Flycatcher; habitat overlap analysis ID SOUTHWESTERN UNITED-STATES; SPECIES DISTRIBUTIONS; RIPARIAN VEGETATION; NORTH-AMERICA; RESTORATION; SALTCEDAR; ECOLOGY; PREDICTION; MODELS; SPP. AB Biologic control of the introduced and invasive, woody plant tamarisk (Tamarix spp, saltcedar) in south-western states is controversial because it affects habitat of the federally endangered South-western Willow Flycatcher (Empidonax traillii extimus). These songbirds sometimes nest in tamarisk where floodplain-level invasion replaces native habitats. Biologic control, with the saltcedar leaf beetle (Diorhabda elongate), began along the Virgin River, Utah, in 2006, enhancing the need for comprehensive understanding of the tamarisk-flycatcher relationship. We used maximum entropy (Maxent) modeling to separately quantify the current extent of dense tamarisk habitat (>50% cover) and the potential extent of habitat available for E. traillii extimus within the studied watersheds. We used transformations of 2008 Landsat Thematic Mapper images and a digital elevation model as environmental input variables. Maxent models performed well for the flycatcher and tamarisk with Area Under the ROC Curve (AUC) values of 0.960 and 0.982, respectively. Classification of thresholds and comparison of the two Maxent outputs indicated moderate spatial overlap between predicted suitable habitat for E. traillii extimus and predicted locations with dense tamarisk stands, where flycatcher habitat will potentially change flycatcher habitats. Dense tamarisk habitat comprised 500 km(2) within the study area, of which 11.4% was also modeled as potential habitat for E. traillii extimus. Potential habitat modeled for the flycatcher constituted 190 km(2), of which 30.7% also contained dense tamarisk habitat. Results showed that both native vegetation and dense tamarisk habitats exist in the study area and that most tamarisk infestations do not contain characteristics that satisfy the habitat requirements of E. traillii extimus. Based on this study, effective biologic control of Tamarix spp. may, in the short term, reduce suitable habitat available to E. traillii extimus, but also has the potential in the long term to increase suitable habitat if appropriate mixes of native woody vegetation replace tamarisk in biocontrol areas. C1 [York, Patricia; Evangelista, Paul; Kumar, Sunil; Graham, James] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Flather, Curtis] US Forest Serv, Dept Agr, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Stohlgren, Thomas] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. RP York, P (reprint author), Colorado State Univ, Nat Resource Ecol Lab, 1499 Campus Delivery, Ft Collins, CO 80523 USA. EM pmyork0714@gmail.com RI Kumar, Sunil/A-6730-2009; Evangelista, Paul/D-2315-2016; Flather, Curtis/G-3577-2012 OI Flather, Curtis/0000-0002-0623-3126 FU US Geological Survey FX We wish to thank the US Geological Survey for funding and the Natural Resources Ecology Laboratory at Colorado State University for logistical support. TJS contribution supported by US Geological Survey Invasive Species Program, USGS Fort Collins Science Program, and USDA CSREES/NRI 2008-35615-04666. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 54 TC 5 Z9 6 U1 3 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2095-0195 J9 FRONT EARTH SCI-PRC JI Front. Earth Sci. PD JUN PY 2011 VL 5 IS 2 BP 120 EP 129 DI 10.1007/s11707-011-0154-5 PG 10 WC Geosciences, Multidisciplinary SC Geology GA 002SS UT WOS:000308556000002 ER PT J AU Liang, CT Stohlgren, TJ AF Liang, Christina T. Stohlgren, Thomas J. TI Habitat suitability of patch types: A case study of the Yosemite toad SO FRONTIERS OF EARTH SCIENCE LA English DT Article DE species distribution models; Maxent; habitat patch; patchy populations; Yosemite toad; Anaxyrus canorus; Bufo canorus ID SPECIES DISTRIBUTIONS; METAPOPULATION DYNAMICS; AMPHIBIAN DECLINES; AMERICAN BULLFROG; CLIMATE-CHANGE; NORTH-AMERICA; SIERRA-NEVADA; CONSERVATION; CALIFORNIA; POPULATIONS AB Understanding patch variability is crucial in understanding the spatial population structure of wildlife species, especially for rare or threatened species. We used a well-tested maximum entropy species distribution model (Maxent) to map the Yosemite toad (Anaxyrus (= Bufo) canorus) in the Sierra Nevada mountains of California. Twenty-six environmental variables were included in the model representing climate, topography, land cover type, and disturbance factors (e. g., distances to agricultural lands, fire perimeters, and timber harvest areas) throughout the historic range of the toad. We then took a novel approach to the study of spatially structured populations by applying the species-environmental matching model separately for 49 consistently occupied sites of the Yosemite toad compared to 27 intermittently occupied sites. We found that the distribution of the entire population was highly predictable (AUC = 0.95+/-0.03 SD), and associated with low slopes, specific vegetation types (wet meadow, alpine-dwarf shrub, montane chaparral, red fir, and subalpine conifer), and warm temperatures. The consistently occupied sites were also associated with these same factors, and they were also highly predictable (AUC = 0.95+/-0.05 SD). However, the intermittently occupied sites were associated with distance to fire perimeter, a slightly different response to vegetation types, distance to timber harvests, and a much broader set of aspect classes (AUC = 0.90+/-0.11 SD). We conclude that many studies of species distributions may benefit by modeling spatially structured populations separately. Modeling and monitoring consistently-occupied sites may provide a realistic snapshot of current species-environment relationships, important climatic and topographic patterns associated with species persistence patterns, and an understanding of the plasticity of the species to respond to varying climate regimes across its range. Meanwhile, modeling and monitoring of widely dispersing individuals and intermittently occupied sites may uncover environmental thresholds and human-related threats to population persistence. C1 [Liang, Christina T.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Liang, Christina T.] US Forest Serv, USDA, Pacific SW Res Stn, Sierra Nevada Res Ctr, Davis, CA 95618 USA. [Stohlgren, Thomas J.] US Geol Survey Sci, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. RP Liang, CT (reprint author), Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. EM christinaliang@fs.fed.us FU University of California at Davis FX Data were provided by the Inyo National Forest, Sierra National Forest, Stanislaus National Forest, Sequoia-Kings Canyon National Park, and Yosemite National Park. We thank R. Grasso for assistance in classifying Yosemite toad locations and S. Kumar for Maxent training. S. Kumar, T. Holcombe, A. Lind, S. Lawler, and J. Quinn provided helpful reviews on an earlier version of the manuscript. CTL funding was provided by the University of California at Davis. TJS contribution supported by US Geological Survey Invasive Species Program, USGS Fort Collins Science Program, and USDA CSREES/NRI 2008-35615-04666. The Natural Resources Ecology Laboratory at Colorado State University provided logistical support. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 39 TC 1 Z9 1 U1 2 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2095-0195 J9 FRONT EARTH SCI-PRC JI Front. Earth Sci. PD JUN PY 2011 VL 5 IS 2 BP 217 EP 228 DI 10.1007/s11707-011-0157-2 PG 12 WC Geosciences, Multidisciplinary SC Geology GA 002SS UT WOS:000308556000012 ER PT J AU Tsilo, TJ Simsek, S Ohm, JB Hareland, GA Chao, SM Anderson, JA AF Tsilo, Toi J. Simsek, Senay Ohm, Jae-Bom Hareland, Gary A. Chao, Shiaoman Anderson, James A. TI Quantitative trait loci influencing endosperm texture, dough-mixing strength, and bread-making properties of the hard red spring wheat breeding lines SO GENOME LA English DT Article DE Wheat; endosperm texture; dough-mixing strength; bread-making properties; quantitative trait loci ID TRITICUM-AESTIVUM L.; DOUBLED HAPLOID POPULATION; PUROINDOLINE SEQUENCE TYPE; GRAIN PROTEIN-CONTENT; X AC-DOMAIN; KERNEL HARDNESS; QUALITY TRAITS; GLUTENIN SUBUNITS; GENETIC-ANALYSIS; BAKING QUALITY AB Wheat end product quality is determined by a complex group of traits including dough viscoelastic characteristics and bread-making properties. Quantitative trait loci (QTL) mapping and analysis were conducted for endosperm texture, dough-mixing strength, and bread-making properties in a population of 139 (MN99394 x MN98550) recombinant inbred lines that were evaluated at three environments in 2006. Based on the genetic map of 534 loci, six QTL were identified for endosperm texture, with the main QTL on chromosomes 1A (R(2) = 6.6%-17.3%), 5A (R(2) = 6.1%-17.1%), and 5D (R(2) = 15.8%-22%). Thirty-four QTL were identified for eight dough-mixing strength and bread-making properties. Major QTL clusters were associated with the low-molecular weight glutenin gene Glu-A3, the two high-molecular weight glutenin genes Glu-B1 and Glu-D1, and two regions on chromosome 6D. Alleles at these QTL clusters have previously been proven useful for wheat quality, except one of the QTL clusters on chromosome 6D. A QTL cluster on chromosome 6D is one of the novel chromosome regions influencing dough-mixing strength and bread-making properties. The QTL for endosperm texture on chromosomes 1A, 5A, and 5B also influenced flour ash content (12.4%-23.3%), flour protein content (10.5%-12.5%), and flour colour (7.7%-13.5%), respectively. C1 [Tsilo, Toi J.; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Simsek, Senay] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Ohm, Jae-Bom; Hareland, Gary A.; Chao, Shiaoman] ARS, Cereal Crops Res Unit, Red River Valley Res Ctr, USDA, Fargo, ND 58105 USA. RP Anderson, JA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall, St Paul, MN 55108 USA. EM ander319@umn.edu RI Tsilo, Toi/G-5088-2011 FU Minnesota Annual Conference of the United Methodist Church; Compton International Foundation; National Research Foundation of South Africa; Agricultural Research Council of South Africa; United States Department of Agriculture-Agricultural Research Service; National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP [2006-55606-16629] FX Financial support for this work was obtained from the Minnesota Annual Conference of the United Methodist Church, the Compton International Foundation, the National Research Foundation of South Africa, the Agricultural Research Council of South Africa, the United States Department of Agriculture-Agricultural Research Service, and the National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP Grant No. 2006-55606-16629. The authors would like to thank G. Linkert, C. Springer, D. Hanson, D. Puhr, and J. Wear for their technical assistance. NR 73 TC 19 Z9 20 U1 1 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0831-2796 J9 GENOME JI Genome PD JUN PY 2011 VL 54 IS 6 BP 460 EP 470 DI 10.1139/G11-012 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 782FW UT WOS:000291994900003 PM 21615298 ER PT J AU Horimoto, T Koshioka, M Kubota, S Mander, LN Hirai, N Ishida, N Suh, JK Lee, AK Roh, MS AF Horimoto, Taiga Koshioka, Masaji Kubota, Satoshi Mander, Lewis N. Hirai, Nobuhiro Ishida, Nobuaki Suh, Jeung Keun Lee, Ae Kyung Roh, Mark S. TI Effect of warm and cold stratification on H-1-NMR profiles, endogenous gibberellins and abscisic acid in Styrax japonicus seeds SO HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY LA English DT Article DE gibberellin metabolism; mass spectrometry; mass spectrometry (UPLC-MS/MS); ultra performance liquid chromatography; water mobility ID FREE-WATER; GERMINATION; INFLORESCENCE; METABOLISM; 20-OXIDASE; RICE AB Germination of Styrax japonicus seeds is promoted by warm stratification (WS) at 18-20A degrees C followed by cold stratification (CS) at 4-5A degrees. The objective of this work was to analyze the state and mobility of water molecules measured by H-1-NMR and endogenous gibberellins (GAs) and abscisic acid (ABA) by ultra performance liquid chromatography/mass spectrometry/mass spectrometry (UPLC-MS/MS) as influenced by WS and CS treatments had not previously been investigated. Styrax seeds that received 35 days of WS (35D WS) followed by 63 days of CS (63D CS) (35D WS + 63D CS) germinated. Seeds that received only 35D WS failed to germinate. Endogenous GA(1), GA(8), GA(19), GA(20), and GA(53) were identified as well as GA(17), GA(23), GA(28), GA(29), and GA(97) by gas chromatography/MS (GC/MS) and UPLC-MS/MS in seeds that were treated with warm and cold stratification (WS + CS). This suggests that the early C-13 hydroxylation pathway [-GA(53)-(GA(44))-GA(19)-GA(20)-GA(1)-GA(8)] of GAs is a major biosynthetic pathway in the seeds. The concentration of GA(53) and GA(19) increased following WS and that of GA(53) increased after WS + CS. The concentration of GA(19) increased only slightly after WS + CS. The concentration of GA(1) increased only after WS + CS. ABA concentration significantly decreased following the WS treatment. It is concluded that the mobility of water molecules and water content in cotyledons and endosperm is increased following WS + CS treatments. The occurrence of C-13 hydroxylated GAs suggests that the early C-13 hydroxylation pathway, -> GA(53) -> GA(44) -> GA(19) -> GA(20) -> GA(1) -> GA(8), is a major biosynthetic pathway in Styrax seeds. C1 [Roh, Mark S.] ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD USA. [Horimoto, Taiga; Koshioka, Masaji; Kubota, Satoshi] Nihon Univ, Coll Bioresource Sci, Dept Plant Sci & Resources, Fujisawa, Kanagawa, Japan. [Mander, Lewis N.] Australian Natl Univ, Res Sch Chem, Canberra, ACT, Australia. [Hirai, Nobuhiro] Kyoto Univ, Grad Sch Agr, Kyoto, Japan. [Ishida, Nobuaki] Ishikawa Prefectural Univ, Dept Food Sci, Nonoichi, Ishikawa, Japan. [Suh, Jeung Keun; Lee, Ae Kyung] Dankook Univ, Coll Bioresources Sci, Dept Environm Hort, Cheonan 330714, South Korea. RP Roh, MS (reprint author), ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD USA. EM Mark.Roh@ars.usda.gov NR 26 TC 4 Z9 4 U1 0 U2 11 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 2211-3452 J9 HORTIC ENVIRON BIOTE JI Hortic. Environ. Biotechnol. PD JUN PY 2011 VL 52 IS 3 BP 233 EP 239 DI 10.1007/s13580-011-0207-4 PG 7 WC Horticulture SC Agriculture GA 782WU UT WOS:000292044200001 ER PT J AU McCreight, JD Coffey, MD AF McCreight, James D. Coffey, Michael D. TI Inheritance of Resistance in Melon PI 313970 to Cucurbit Powdery Mildew Incited by Podosphaera xanthii Race S SO HORTSCIENCE LA English DT Article DE Cucumis melo; Sphaerotheca fuliginea; resistant blister ID CICHORACEARUM AB A new strain of cucurbit powdery mildew incited by Podosphaera xanthii (Castagne) Braun & Shishkoff (formerly Sphaerotheca fuliginea), designated race S, is virulent on all the commonly used melon (Cucumis melo L.) powdery mildew race differentials and first appeared on melon in Yuma, AZ, and Imperial Valley, CA, in 2003. Melon PI 313970 (C. melo var. acidulus) was resistant to P. xanthii race S in 2003 and subsequent years. Inheritance of resistance to P. xanthii race S was studied in two naturally infected, replicated field tests in Imperial Valley in 2005 and 2009 using the parents and F(1), F(2), and reciprocal backcross generations from crosses of PI 313970 with powdery mildew-susceptible 'Top Mark'. Resistance to race S was recessive: all F1 and BC(TM) individuals were susceptible. One recessive gene, designated pm-S, conditioned resistance to race S in the F(2) and BC(PI),. The relationship of pm-S to the previously reported recessive and codominant genes in PI 313970 for resistance to P. xanthii races 1, 2, 2U.S., 3, 3.5, 4.5, and 5 remains to be determined. C1 [McCreight, James D.] ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA. RP McCreight, JD (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM jim.mccreight@ars.usda.gov FU California Melon Research Board FX Funded in part by a grant from the California Melon Research Board. NR 21 TC 9 Z9 10 U1 3 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 838 EP 840 PG 3 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800002 ER PT J AU Castle, WS Bowman, KD Baldwin, JC Grosser, JW Gmitter, FG AF Castle, William S. Bowman, Kim D. Baldwin, James C. Grosser, Jude W. Gmitter, Frederick G., Jr. TI Rootstocks Affect Tree Growth, Yield, and Juice Quality of 'Marsh' Grapefruit SO HORTSCIENCE LA English DT Article DE Citrange; citrumelo; mandarin rootstocks; Sunki mandarin hybrids; US-897; US-812 ID VALENCIA SWEET ORANGE; PERFORMANCE; CITRUS; SELECTION; LOCATIONS; MANDARIN; HAMLIN; HABIT AB Two adjacent rootstock trials were conducted in the east coast Indian River region of Florida with 'Marsh' grapefruit (Citrus paradisi Macf.) scion. The objective was to find rootstocks to replace sour orange (C. aurantium L.) because of losses to citrus tristeza virus, and to replace Swingle citrumelo [C. paradisi x Poncirus trifoliata (L.) Raf.] because of its limited usefulness in certain poorly drained coastal sites. The trials were conducted in randomized complete blocks with 12 single-tree replicates spaced 4.6 x 6.9 m. The soils were of the Wabasso and Riviera series. The first trial consisted largely of trees on citrange [C. sinensis (L.) Osb. x P. trifiliata] and citrumelo rootstocks, 'Cipo' sweet orange (C. sinensis), and various hybrid rootstocks. The second trial involved mandarin rootstocks (C. reticulata Blanco) and sour orange and related rootstocks. Trees were grown for 7 years and yield and juice quality data were collected for the last 4 years of that period. Those rootstocks identified as the most promising, based on combinations of smaller tree size and high productivity and juice quality, were two Sunki mandarin x Swingle trifoliate orange (TF) hybrids (C-54, C-146), a Sunki mandarin x Flying Dragon TF hybrid, C-35 citrange, and a Cleopatra mandarin x Rubidoux TF hybrid (x639). The trees on these five rootstocks cropped well leading to soluble solids (SS) values of 3000 to 4000 kg/ha when they were 7-years old. The trees on C-54 and C-146 were relatively large, somewhat taller than trees on sour orange, whereas those on C-35 and the Sunki x Flying Dragon hybrid were smaller and similar to sour orange in tree height. Fruit quality among the trees on C-35 and the Sunki x Flying Dragon hybrid had relatively high SS concentration (better than sour orange), and the other three rootstocks had relatively lower solids concentration (poorer than sour orange). The trees on C-35 and the Sunki x Flying Dragon hybrid would be good candidates for higher density orchards. C1 [Castle, William S.; Baldwin, James C.; Grosser, Jude W.; Gmitter, Frederick G., Jr.] Univ Florida, Citrus Res & Educ Ctr, IFAS, Dept Hort Sci, Lake Alfred, FL 33850 USA. [Bowman, Kim D.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Castle, WS (reprint author), Univ Florida, Citrus Res & Educ Ctr, IFAS, Dept Hort Sci, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM bcastle@ufl.edu FU Florida Citrus Production Research Advisory Council FX We are very grateful to personnel of the Becker Holding Company on whose property this work was conducted. We especially recognize Lynn Faulkner who at the time was the nursery manager and oversaw the considerable effort in propagating the trial trees and insuring their identification through to planting. We also extend our gratitude to Florida citrus growers who supported this work through a self-imposed tax for research funds granted by the Florida Citrus Production Research Advisory Council. NR 40 TC 5 Z9 5 U1 1 U2 13 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 841 EP 848 PG 8 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800003 ER PT J AU Stover, EW McCollum, TG AF Stover, E. W. McCollum, T. G. TI Levels of Candidatus Liberibacter asiaticus and Xanthomonas citri in Diverse Citrus Genotypes and Relevance to Potential Transmission from Pollinations SO HORTSCIENCE LA English DT Article DE breeding; citrus canker; huanglonging; HLB ID REAL-TIME PCR AB The diseases huanglongbing [HLB, associated with Candidatus Liberibacter asiaticus (CLas)] and Asian citrus canker [ACC, caused by Xantlzomonas citri (Xcc)] are widespread in Florida and many other citrus-growing areas, presenting unprecedented challenges for citrus breeding. Because RIB and ACC weaken trees and compromise cropping, breeding is much less efficient using seed parents that have been exposed to these diseases. Therefore, it would be highly desirable to use unique disease-exposed selections only as pollen parents with pollen applied to disease-free trees. However, there may be a risk of introducing these diseases using such pollen sources. To assess this potential, abundance of the pathogens associated with these diseases was assessed in anthers and flowers using quantitative polymerase chain reaction. Because CLas is systemic, levels on mature leaves from the flower source trees were assessed to see if the presence of CLas in flowers was associated with leaf levels. Disease-exposed trees were tested in 10 genotypes from each of three broad genotypic categories, which reflect different levels of susceptibility to the diseases associated with the pathogens studied: Ponciras trifoliata hybrids (most resistant to HLB), Citrus maxima and hybrids (susceptible to both diseases), and C. reticulata and hybrids (considerable resistance to ACC). Of the 30 samples of each tissue type analyzed for CLas, 88% of mature leaves, 69% of flowers, and 88% of anthers had one or more CLas bacterium per sample. The trifoliate genotypic group had significantly lower levels of CLas than the pummelo and mandarin groups in mature leaf samples, but CLas levels were more similar between groups in anther and flower samples, and the pathogen was present in most of the trifoliate hybrids tested. Mean numbers of CLas detected per nanogram nucleic acid were 100 to 800 times higher in mature leaf samples, most characteristic of HLB symptoms, compared with anther samples. Xcc DNA was detected in 30% of flower samples and 23% of anther samples. No significant differences in Xcc levels were found between tissue type or genotypic group. However, regressions between Xcc levels in flowers and percent of plant pedigree derived from mandarin had a negative correlation and an r(2) of 0.159 (P= 0.029). The biology of CLas is consistent with the pathogen being present in anthers from unopened flowers, whereas the ACC pathogen detected inside flowers was likely the result of contamination despite great care in sample collection and handling. Where exceptional diligence to exclude HLB and ACC is appropriate, results suggest that there may be a risk of spreading these pathogens through use of pollen from trees on infected farms. C1 [Stover, E. W.; McCollum, T. G.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Stover, EW (reprint author), ARS, USDA, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov NR 16 TC 4 Z9 4 U1 0 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 854 EP 857 PG 4 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800005 ER PT J AU Farnham, MW Bjorkman, T AF Farnham, Mark W. Bjoerkman, Thomas TI Evaluation of Experimental Broccoli Hybrids Developed for Summer Production in the Eastern United States SO HORTSCIENCE LA English DT Article DE Brassica oleracea Italica Group; heat tolerance; environmental stress; vernalization ID VAR. ITALICA; TEMPERATURE; INFLORESCENCE; INITIATION; MODEL; L. AB Broccoli (Brassica oleracea L. Italica Group) is a vegetable crop requiring relatively cool conditions (e.g., less than 23 degrees C) to induce and maintain vernalization and to allow normal floral and head development to proceed. In general, this requirement is a major limiting factor to production of broccoli in eastern states where growing seasons are often interrupted by high temperature spikes. The USDA, ARS, U.S. Vegetable Laboratory (USVL) is conducting a program to breed broccoli varieties adapted to summer conditions of the southeastern United States. The goal of the current study was to compare performance of three experimental broccoli hybrids from that program with some commonly raised commercial hybrids ('Packman', 'Marathon', 'Arcadia', 'Greenbelt', 'Patron', and 'Gypsy') by conducting trials in summer environments as well as in more conventional growing environments (e.g., in fall). All hybrids produced marketable heads with high quality ratings in fall field trials (2006, 2007, and 2008). Under the high temperatures that were characteristic of the summer (2007, 2008, and 2009) trials in South Carolina, the commercial hybrids 'Marathon', 'Greenbelt', 'Arcadia', and 'Patron' failed to produce broccoli heads at all. The remaining hybrids produced heads with similar mean head mass, stem diameter, and bead size in South Carolina summer trials. However, the three experimental hybrids produced marketable quality heads, but 'Gypsy' and 'Packman' did not. The primary flaws in 'Gypsy' and 'Packman' heads were increased yellow color, flattening of the dome, increased roughness, and non-uniformity of bead size. In New York trials, all tested hybrids developed heads, but 'Packman' and 'Marathon' produced relatively poor-quality heads when maturing in summer and better quality heads when maturing in the fall. The experimental hybrids exhibited more consistent quality across different maturity times in the New York tests. Results of this research indicate that broccoli response to summer conditions of the eastern United States is dependent on the cultivar grown. Many cultivars are not adapted to extreme summer conditions of the Southeast because they will not be effectively vernalized and will therefore not head. Others such as 'Gypsy' and 'Packrnan' will head, but non-uniform bud development results in a rough-appearing curd in which flower buds are at various stages of development. The experimental hybrids that are single crosses of inbreds selected for adaptation to southeastern summer conditions represent a unique class of broccoli hybrids that combine early maturity and the ability to produce heads under summer conditions of South Carolina. Additional tests of these latter hybrids in New York indicate that they may be generally adapted to summer environments of the eastern United States. C1 [Farnham, Mark W.] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. [Bjoerkman, Thomas] Cornell Univ, Dept Hort, NYSAES, Geneva, NY 14456 USA. RP Farnham, MW (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Mark.Farnham@ars.usda.gov RI Bjorkman, Thomas/D-1047-2012 OI Bjorkman, Thomas/0000-0001-7151-6205 NR 16 TC 8 Z9 8 U1 0 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 858 EP 863 PG 6 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800006 ER PT J AU Wood, BW AF Wood, Bruce W. TI Influence of Plant Bioregulators on Pecan Flowering and Implications for Regulation of Pistillate Flower Initiation SO HORTSCIENCE LA English DT Article DE growth regulators; fruiting; flowering; alternate bearing; irregular bearing; profit; production; flowers; staminate flowers ID GIBBERELLIN-LIKE SUBSTANCES; ALTERNATE-BEARING; GENE-EXPRESSION; RETURN BLOOM; FLORAL INITIATION; FALL DEFOLIATION; FRUIT-QUALITY; BUD FORMATION; LATERAL BUDS; APPLE AB Mitigation of alternate bearing (AB) through regulation of floral initiation of pistillate flowers is central to improving cropload management of pecan [Carya illinoinensis (Wangenh.) K. Koch] trees and orchards. The present study examines the influence of key bioregulators {i.e., an auxin [as B-napththaleneacetic acid (NAA)], a cytokinin [6-benylamino purine (6-BA)], an ethylene generator (ethephon), and an auxin transport inhibitor [2,3,5-triiodobenzoic acid (TIBA)]} on subsequent season pistillate flowering. Gibberellic acid (i.e., GA(3)) and NAA inhibited, whereas prohexadione-calcium (P-Ca; calcium 3-oxido-5-oxo-4-propionylcyclohex-3-enecarboxylate), ethephon, and BA + TIBA promoted floral initiation when topically applied to canopies before the kernel filling stage of seed development. These bioregulators exhibit potential for integration into a bioregulator-based strategy to mitigate pecan AB by selective and timely use in "off" or "on" cycle years, depending on the bioregulator. Field studies provide evidence that a "cytokinin-gibberellin balance," with partial modulation by auxin and ethylene, acts in the endogenous primordial environment of floral meristems as a "second-level signal" regulating a key step in a three-step process for initiation of pistillate flowers in pecan. This establishes a new model for explaining pistillate flower initiation in pecan and a basis for designing future research on the control and management of pistillate flowering and AB. C1 ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Wood, BW (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM bruce.wood@ars.usda.gov NR 57 TC 10 Z9 10 U1 3 U2 14 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 870 EP 877 PG 8 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800008 ER PT J AU Wall, MM Nishijima, KA Keith, LM Nagao, MA AF Wall, Marisa M. Nishijima, Kate A. Keith, Lisa M. Nagao, Mike A. TI Influence of Packaging on Quality Retention of Longans (Dimocarpus longan) Under Constant and Fluctuating Postharvest Temperatures SO HORTSCIENCE LA English DT Article DE Sapindaceae; tropical fruit; postharvest; modified atmosphere packaging ID FRUIT; STORAGE; VEGETABLES; PERICARP; OXYGEN; WATER; ACID AB Longan (Dimcarpus longan) fruit production and global exports are rapidly expanding. Consumer acceptance of this high value crop requires that fruit arrive in excellent condition. Pericarp browning and fungal diseases are the main postharvest problems for longans. Research was conducted to establish optimum storage temperatures and packaging systems to retain fruit quality of 'Biew Kiew' longans. Average respiration rates for longans stored at 20 degrees C (61.6 mg CO(2)/kg/h) were about twice the rate as those stored at 10 degrees C (32.7 mg CO(2)/kg/h) and triple the rate for those stored at 5 degrees C (21.1 mg CO(2)/kg/h). Ethylene rates were below 0.4 mu g-kg(-1).h(-1). Fruit quality and shelf life were greatest when stored at 10 degrees C. Longans held at 20 degrees C were unmarketable after 10 d, and fruit stored at 5 C exhibited chilling injury (CI). After storage at 10 degrees C, longans packaged in microperforated (MP) bags, clamshell (CL) containers, or Peakfresh (R) film (PF) had the highest visual quality ratings, lowest disease incidences, and longest shelf life when compared with fruit in Lifespan (R) film (LS) or fiberboard boxes. The most promising packages (MP, CL, PF) were evaluated further under constant 10 degrees C or simulated shipping (SS) conditions with fluctuating temperatures (22 degrees C/10 degrees C/22 degrees C). Longans in CL containers had the highest visual quality and lowest disease incidence when stored at 10 degrees C, but there were no differences among package treatments under SS conditions. Also, sensory ratings were greatest for fruit packed in CL or PF when stored at 10 degrees C but all sensory scores decreased under SS temperatures. When longans were stored under fluctuating temperatures, aril texture and flavor ratings were highest for CL packages. CL, PF, and MP are suitable packages for longans stored under optimal temperatures. However, for longans stored under SS conditions, sensory quality was highest when packaged in CL containers. C1 [Wall, Marisa M.; Nishijima, Kate A.; Keith, Lisa M.] ARS, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Nagao, Mike A.] Univ Hawaii, Beaumont Agr Res Ctr, Coll Trop Agr & Human Resources, Hilo, HI 96720 USA. RP Wall, MM (reprint author), ARS, USDA, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM marisa.wall@ars.usda.gov FU USDA; USDA Agricultural Research Service FX This research was supported by the USDA Special Research Grant for Agricultural Diversification: Hawaii Tropical Specialty Fruit Research and Development and by the USDA Agricultural Research Service. NR 30 TC 1 Z9 1 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 917 EP 923 PG 7 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800016 ER PT J AU Fery, RL Thies, JA AF Fery, Richard L. Thies, Judy A. TI PA-560, a Southern Root-knot Nematode-resistant, Yellow-fruited, Habanero-type Pepper SO HORTSCIENCE LA English DT Editorial Material DE Capsicum chinense; Meloidogvne incognita; vegetable breeding ID CAPSICUM-CHINENSE JACQ C1 [Fery, Richard L.; Thies, Judy A.] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. RP Fery, RL (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Richard.Fery@ars.usda.gov NR 4 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2011 VL 46 IS 6 BP 946 EP 947 PG 2 WC Horticulture SC Agriculture GA 782OU UT WOS:000292019800021 ER PT J AU Hawksworth, DL Crous, PW Redhead, SA Reynolds, DR Samson, RA Seifert, KA Taylor, JW Wingfield, MJ Abaci, O Aime, C Asan, A Bai, FY de Beer, ZW Begerow, D Berikten, D Boekhout, T Buchanan, PK Burgess, T Buzina, W Cai, L Cannon, PF Crane, JL Damm, U Daniel, HM van Diepeningen, AD Druzhinina, I Dyer, PS Eberhardt, U Fell, JW Frisvad, JC Geiser, DM Geml, J Glienke, C Grafenhan, T Groenewald, JZ Groenewald, M de Gruyter, J Gueho-Kellermann, E Guo, LD Hibbett, DS Hong, SB de Hoog, GS Houbraken, J Huhndorf, SM Hyde, KD Ismail, A Johnston, PR Kadaifciler, DG Kirk, PM Koljalg, U Kurtzman, CP Lagneau, PE Levesque, CA Liu, XZ Lombard, L Meyer, W Miller, A Minter, DW Najafzadeh, MJ Norvell, L Ozerskaya, SM Ozic, R Pennycook, SR Peterson, SW Pettersson, OV Quaedvlieg, W Robert, VA Ruibal, C Schnurer, J Schroers, HJ Shivas, R Slippers, B Spierenburg, H Takashima, M Taskin, E Thines, M Thrane, U Uztan, AH van Raak, M Varga, J Vasco, A Verkley, G Videira, SIR de Vries, RP Weir, BS Yilmaz, N Yurkov, A Zhang, N AF Hawksworth, David L. Crous, Pedro W. Redhead, Scott A. Reynolds, Don R. Samson, Robert A. Seifert, Keith A. Taylor, John W. Wingfield, Michael J. Abaci, Ozlem Aime, Catherine Asan, Ahmet Bai, Feng-Yan de Beer, Z. Wilhelm Begerow, Dominik Berikten, Derya Boekhout, Teun Buchanan, Peter K. Burgess, Treena Buzina, Walter Cai, Lei Cannon, Paul F. Crane, J. Leland Damm, Ulrike Daniel, Heide-Marie van Diepeningen, Anne D. Druzhinina, Irina Dyer, Paul S. Eberhardt, Ursula Fell, Jack W. Frisvad, Jens C. Geiser, David M. Geml, Jozsef Glienke, Chirlei Grafenhan, Tom Groenewald, Johannes Z. Groenewald, Marizeth de Gruyter, Johannes Gueho-Kellermann, Eveline Guo, Liang-Dong Hibbett, David S. Hong, Seung-Beom de Hoog, G. Sybren Houbraken, Jos Huhndorf, Sabine M. Hyde, Kevin D. Ismail, Ahmed Johnston, Peter R. Kadaifciler, Duygu G. Kirk, Paul M. Koljalg, Urmas Kurtzman, Cletus P. Lagneau, Paul-Emile Levesque, C. Andre Liu, Xingzhong Lombard, Lorenzo Meyer, Wieland Miller, Andrew Minter, David W. Najafzadeh, Mohammad Javad Norvell, Lorelei Ozerskaya, Svetlana M. Ozic, Rasime Pennycook, Shaun R. Peterson, Stephen W. Pettersson, Olga V. Quaedvlieg, William Robert, Vincent A. Ruibal, Constantino Schnurer, Johan Schroers, Hans-Josef Shivas, Roger Slippers, Bernard Spierenburg, Henk Takashima, Masako Taskin, Evrim Thines, Marco Thrane, Ulf Uztan, Alev Haliki van Raak, Marcel Varga, Janos Vasco, Aida Verkley, Gerard Videira, Sandra I. R. de Vries, Ronald P. Weir, Bevan S. Yilmaz, Neriman Yurkov, Andrey Zhang, Ning TI The Amsterdam Declaration on Fungal Nomenclature SO IMA FUNGUS LA English DT Article DE Anamorph; Article 59; BioCode; Candidate species; Environmental sequences; International Code of Botanical Nomenclature; MycoCode; Pleomorphic fungi; Teleomorph AB The Amsterdam Declaration on Fungal Nomenclature was agreed at an international symposium convened in Amsterdam on 19-20 April 2011 under the auspices of the International Commission on the Taxonomy of Fungi (ICTF). The purpose of the symposium was to address the issue of whether or how the current system of naming pleomorphic fungi should be maintained or changed now that molecular data are routinely available. The issue is urgent as mycologists currently follow different practices, and no consensus was achieved by a Special Committee appointed in 2005 by the International Botanical Congress to advise on the problem. The Declaration recognizes the need for an orderly transitition to a single-name nomenclatural system for all fungi, and to provide mechanisms to protect names that otherwise then become endangered. That is, meaning that priority should be given to the first described name, except where that is a younger name in general use when the first author to select a name of a pleomorphic monophyletic genus is to be followed, and suggests controversial cases are referred to a body, such as the ICTF, which will report to the Committee for Fungi. If appropriate, the ICTF could be mandated to promote the implementation of the Declaration. In addition, but not forming part of the Declaration, are reports of discussions held during the symposium on the governance of the nomenclature of fungi, and the naming of fungi known only from an environmental nucleic acid sequence in particular. Possible amendments to the Draft BioCode (2011) to allow for the needs of mycologists are suggested for further consideration, and a possible example of how a fungus only known from the environment might be described is presented. C1 [Hawksworth, David L.; Ruibal, Constantino] Univ Complutense Madrid, Fac Farm, Dept Biol Vegetal 2, Plaza Ramon y Cajal, E-28040 Madrid, Spain. [Hawksworth, David L.; Ruibal, Constantino] Nat Hist Museum, Dept Bot, Cromwell Rd, London SW7 5BD, England. [Crous, Pedro W.; Samson, Robert A.; Boekhout, Teun; Damm, Ulrike; van Diepeningen, Anne D.; Eberhardt, Ursula; Groenewald, Johannes Z.; Groenewald, Marizeth; de Hoog, G. Sybren; Houbraken, Jos; Ismail, Ahmed; Lombard, Lorenzo; Quaedvlieg, William; Robert, Vincent A.; Spierenburg, Henk; Verkley, Gerard; Videira, Sandra I. R.; de Vries, Ronald P.; Yilmaz, Neriman] CBS KNAW Fungal Biodivers Ctr, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands. [Redhead, Scott A.; Seifert, Keith A.] Agr & Agri Food Canada, Natl Mycol Herbarium, Neatby Bldg,960 Carling Ave, Ottawa, ON K1A 0C6, Canada. [Reynolds, Don R.] Univ Calif Berkeley, Herbarium, 1001 Valley Life Sci Bldg 2465, Berkeley, CA 94720 USA. [Taylor, John W.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Wingfield, Michael J.; de Beer, Z. Wilhelm; Slippers, Bernard] Univ Pretoria, Forestry & Agr Biotechnol Inst, Private Bag X20,Hatfield 0028, ZA-0002 Pretoria, South Africa. [Abaci, Ozlem] Ege Univ, Fac Sci, Dept Biol, Basic & Ind Microbiol Sect, Izmir, Turkey. [Aime, Catherine] Louisiana State Univ, Dept Plant Pathol & Crop Physiol, Ctr Agr, 302 Life Sci Bldg, Baton Rouge, LA 70803 USA. [Asan, Ahmet] Trakya Univ, Dept Biol, TR-22030 Edirne, Turkey. [Bai, Feng-Yan; Guo, Liang-Dong; Liu, Xingzhong] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, 3,1st Beichen West Rd, Beijing 100101, Peoples R China. [Begerow, Dominik; Yurkov, Andrey] Ruhr Univ Bochum, AG Geobot, Univ Str 150, D-44780 Bochum, Germany. [Berikten, Derya] Anadolu Univ, Dept Biol, TR-26470 Eskisehir, Turkey. [Buchanan, Peter K.; Weir, Bevan S.] Landcare Res, Private Bag 92170, Auckland 1142, New Zealand. [Burgess, Treena] Murdoch Univ, Sch Biol Sci & Biotechnol, South St, Murdoch, WA 6150, Australia. [Buzina, Walter] Med Univ Graz, Inst Hyg Microbiol & Environm Med, Univ Pl 4, A-8010 Graz, Austria. [Cai, Lei] Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, 10 North 4th Ring Rd West, Beijing 100190, Peoples R China. [Cannon, Paul F.] Royal Bot Gardens, Jodrell Lab, CABI Europe UK, Richmond TW9 3AB, Surrey, England. [Cannon, Paul F.] Royal Bot Gardens, Jodrell Lab, Royal Bot Gardens Kew, Richmond TW9 3AB, Surrey, England. [Daniel, Heide-Marie] Catholic Univ Louvain, Earth & Life Inst, BCCM MUCL, Appl Microbiol,Mycol, Croix Sud 3,Bte 6, B-1348 Louvain La Neuve, Belgium. [Druzhinina, Irina] Vienna Univ Technol, Inst Chem Engn, Area Gene Technol & Appl Biochem, A-1060 Vienna, Austria. [Dyer, Paul S.] Univ Nottingham, Sch Biol, Univ Pk, Nottingham NG7 2RD, England. [Fell, Jack W.] Univ Miami, RSMAS, 4600 Rickenbacker Causeway, Key Biscayne, FL 33149 USA. [Frisvad, Jens C.; Thrane, Ulf] Tech Univ Denmark, Dept Syst Biol, Ctr Microbial Biotechnol, Soltofts Plads B 221, DK-2800 Lyngby, Denmark. [Geiser, David M.] Penn State Univ, Dept Plant Pathol, Fusarium Res Ctr, University Pk, PA 16802 USA. [Geml, Jozsef] Leiden Univ, Netherlands Ctr Biodivers Naturalis, Natl Herbarium Netherlands, POB 9514,Einsteinweg 2, NL-2300 RA Leiden, Netherlands. [Glienke, Chirlei] Univ Fed Parana, Dept Genet, POB 19071, BR-81531099 Curitiba, Parana, Brazil. [Grafenhan, Tom] Canadian Grain Commiss, Grain Res Lab, 1404-303 Main St, Winnipeg R3C 3G8, MB, Canada. [de Gruyter, Johannes; van Raak, Marcel] Plant Protect Serv, POB 9102, NL-6700 HC Wageningen, Netherlands. [Hibbett, David S.] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Hong, Seung-Beom] Natl Acad Agr Sci, Suwon 441707, South Korea. [Huhndorf, Sabine M.] Field Museum, Dept Bot, 400 South Lake Shore Dr, Chicago, IL 60605 USA. [Kadaifciler, Duygu G.] Istanbul Univ, Fac Sci, Dept Biol, TR-34134 Vezneciler, Turkey. [Kirk, Paul M.] CABI Europe, Bakeham Lane, Surrey TW20 9TY, England. [Koljalg, Urmas] Univ Tartu, Inst Ecol & Earth Sci, 40 Lai St, EE-51005 Tartu, Estonia. [Kurtzman, Cletus P.; Peterson, Stephen W.] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Lagneau, Paul-Emile] Reg Assoc Hlth & Anim Identificat, Dreve Prophete 2, B-7000 Mons, Belgium. [Meyer, Wieland] Univ Sydney, Ctr Infect Dis & Microbiol, Westmead Hosp,Mol Mycol Res Lab, ICPMR,Westmead Millennium Inst,Sydney Med Sch Wes, Level 3,Room 3114A,Darcy Rd, Westmead, NSW 2145, Australia. [Miller, Andrew] Univ Illinois, Illinois Nat Hist Survey, 1816 South Oak St, Champaign, IL 61820 USA. [Minter, David W.] Cybertruffle, 4 Esk Terrace, Whitby YO21 1PA, N Yorkshire, England. [Minter, David W.] CAB Int, Bakeham Lane, Egham TW20 9TY, Surrey, England. [Najafzadeh, Mohammad Javad] Mashhad Univ Med Sci, Mashhad, Iran. [Ozerskaya, Svetlana M.] GK Skryabin Inst Biochem & Physiol Microorganisms, All Russian Collect Microorganisms, Prospect Nauki 5, Pushchino 142290, Russia. [Ozic, Rasime] Anadolu Univ, Fac Sci, Dept Biol, TR-26470 Eskisehir, Turkey. [Pettersson, Olga V.; Schnurer, Johan] Swedish Univ Agr Sci, Uppsala Bioctr, Dept Microbiol, POB 7025, SE-75007 Uppsala, Sweden. [Schroers, Hans-Josef] Agr Inst Slovenia, Hacquetova 17, Ljubljana 1000, Slovenia. [Shivas, Roger] Ecosci Precinct, Dept Employment Econ Dev & Innovat, Plant Pathol Herbarium BRIP, 41 Boggo Rd, Dutton Park, Qld 4102, Australia. [Takashima, Masako] RIKEN, BioResource Ctr, Japan Collect Microorganisms, 2-1 Hirosawa, Wako, Saitama 3510198, Japan. [Taskin, Evrim] Celal Bayar Univ, Fac Arts & Sci, Dept Biol, TR-45140 Muradiye, Turkey. [Thines, Marco] Biodivers & Climate Res Ctr BiK F, Senckenberganlage 25, D-60325 Frankfurt, Germany. [Thines, Marco] Goethe Univ, Inst Ecol Evolut & Div, Siesmayerstr 70, D-60323 Frankfurt, Germany. [Uztan, Alev Haliki] Ege Univ, Dept Biol, Basic & Ind Microbiol Sect, Bornova, Turkey. [Varga, Janos] Univ Szeged, Fac Sci & Informat, Dept Microbiol, Kozep Fasor 52, H-6726 Szeged, Hungary. [Vasco, Aida] Univ Antioquia, Fac Ciencias Exactas & Nat, Inst Biol, Lab Taxon & Ecol Hongos, Medellin 1226, Colombia. [Zhang, Ning] Rutgers State Univ, Dept Plant Biol & Pathol, 59 Dudley Rd, New Brunswick, NJ 08901 USA. RP Hawksworth, DL (reprint author), Univ Complutense Madrid, Fac Farm, Dept Biol Vegetal 2, Plaza Ramon y Cajal, E-28040 Madrid, Spain. EM d.hawksworth@nhm.ac.uk; p.crous@cbs.knaw.nl RI CAI, LEI/K-7226-2014; de Beer, Z. Wilhelm/B-6353-2008; Lombard, Lorenzo/B-2042-2010; Crous, Pedro/H-1489-2012; Thrane, Ulf/G-2978-2016; Zhang, Ning/K-3046-2012; Burgess, Treena/G-4770-2011; Videira, Sandra/C-8593-2013; Quaedvlieg, William/C-9449-2013; Weir, Bevan/B-5057-2008; Groenewald, Marizeth/G-4976-2012; Groenewald, Johannes/F-4667-2011; OI CAI, LEI/0000-0002-8131-7274; de Beer, Z. Wilhelm/0000-0001-9758-8987; Lombard, Lorenzo/0000-0002-5942-5375; Crous, Pedro/0000-0001-9085-8825; Thrane, Ulf/0000-0002-6040-4141; Zhang, Ning/0000-0003-0755-2505; Burgess, Treena/0000-0002-7962-219X; Videira, Sandra/0000-0002-4632-0777; Weir, Bevan/0000-0003-2580-0701; Groenewald, Marizeth/0000-0003-0835-5925; Yurkov, Andrey/0000-0002-1072-5166; de Vries, Ronald/0000-0002-4363-1123; Meyer, Wieland/0000-0001-9933-8340; Ruibal Villasenor, Constantino/0000-0003-3494-7051 FU CBS-KNAW Fungal Biodiversity Centre FX The mycological community is indebted to Keith A Seifert and Robert A Samson for conceiving and organizing the One Fungus = One Name symposium. The CBS-KNAW Fungal Biodiversity Centre kindly provided logistical and financial support towards the organization of the symposium. NR 31 TC 144 Z9 157 U1 0 U2 13 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2011 VL 2 IS 1 BP 105 EP 112 DI 10.5598/imafungus.2011.02.01.14 PG 8 WC Mycology SC Mycology GA V46TR UT WOS:000209907000037 PM 22679594 ER PT J AU Yuan, YP Bingner, RL Locke, MA Stafford, J Theurer, FD AF Yuan, Yongping Bingner, Ronald L. Locke, Martin A. Stafford, Jim Theurer, Fred D. TI Assessing the Long Term Impact of Phosphorus Fertilization on Phosphorus Loadings Using AnnAGNPS SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article; Proceedings Paper CT 7th International Symposium on Recent Advances in Environmental Health Research CY SEP 12-15, 2010 CL Jackson State Univ (JSU), Jackson, MI SP Natl Inst Hlth (NIH), RCMI-Ctr Environm Hlth, Environm Hlth Protect Agcy, JSU Off Acad Affairs, JSU Off Res & Fed Relations HO Jackson State Univ (JSU) DE AnnAGNPS watershed modeling; phosphorus fertilization rates; phosphorus loss ID SOIL-PHOSPHORUS; RUNOFF PHOSPHORUS; MODEL; ACCUMULATION; WATERSHEDS; COMPONENT; ALBERTA; LOSSES AB High phosphorus (P) loss from agricultural fields has been an environmental concern because of potential water quality problems in streams and lakes. To better understand the process of P loss and evaluate the effects of different phosphorus fertilization rates on phosphorus losses, the USDA Annualized AGricultural Non-Point Source (AnnAGNPS) pollutant loading model was applied to the Ohio Upper Auglaize watershed, located in the southern portion of the Maumee River Basin. In this study, the AnnAGNPS model was calibrated using USGS monitored data; and then the effects of different phosphorus fertilization rates on phosphorus loadings were assessed. It was found that P loadings increase as fertilization rate increases, and long term higher P application would lead to much higher P loadings to the watershed outlet. The P loadings to the watershed outlet have a dramatic change after some time with higher P application rate. This dramatic change of P loading to the watershed outlet indicates that a. critical point. may exist in the soil at which soil P loss to water changes dramatically. Simulations with different initial soil P contents showed that the higher the initial soil P content is, the less time it takes to reach the. critical point. where P loadings to the watershed outlet increases dramatically. More research needs to be done to understand the processes involved in the transfer of P between the various stable, active and labile states in the soil to ensure that the model simulations are accurate. This finding may be useful in setting up future P application and management guidelines. C1 [Yuan, Yongping] US EPA, Div Environm Sci, Off Res & Dev, Las Vegas, NV 89119 USA. [Bingner, Ronald L.; Locke, Martin A.] USDA ARS, Water Qual & Ecol Res Unit, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Stafford, Jim] USDA, NRCS, Columbus, OH 43215 USA. [Theurer, Fred D.] USDA, Water Qual & Quant Team, NRCS, Beltsville, MD 20705 USA. RP Yuan, YP (reprint author), US EPA, Div Environm Sci, Off Res & Dev, 944 E Harmon Ave, Las Vegas, NV 89119 USA. EM yuan.yongping@epa.gov; ron.bingner@ars.usda.gov; martin.locke@ars.usda.gov; jim.stafford@oh.usda.gov; Fred.Theurer@verizon.net RI feng, yongzhong/F-5090-2012 OI feng, yongzhong/0000-0002-5202-4368 NR 32 TC 6 Z9 7 U1 2 U2 19 PU MDPI AG PI BASEL PA KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD JUN PY 2011 VL 8 IS 6 BP 2181 EP 2199 DI 10.3390/ijerph8062181 PG 19 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA 782PV UT WOS:000292022500026 PM 21776225 ER PT J AU Labeda, DP Goodfellow, M Chun, J Zhi, XY Li, WJ AF Labeda, D. P. Goodfellow, M. Chun, J. Zhi, X. -Y. Li, W. -J. TI Reassessment of the systematics of the suborder Pseudonocardineae: transfer of the genera within the family Actinosynnemataceae Labeda and Kroppenstedt 2000 emend. Zhi et al. 2009 into an emended family Pseudonocardiaceae Embley et al. 1989 emend. Zhi et al. 2009 SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID GEN. NOV.; COMB. NOV; ORDER ACTINOMYCETALES; PROPOSAL; AMYCOLATOPSIS; SACCHAROTHRIX; STREPTOALLOTEICHUS; MEMBER; SOIL; ACTINOALLOTEICHUS AB The taxonomic status of the families Actinosynnemataceae and Pseudonocardiaceae was assessed based on 16S rRNA gene sequence data available for the 151 taxa with validly published names, as well as chemotaxonomic and morphological properties available from the literature. 16S rRNA gene sequences for the type strains of all taxa within the suborder Pseudonocardineae were subjected to phylogenetic analyses using different algorithms in ARB and PHYLIP. The description of many new genera and species within the suborder Pseudonocardineae since the family Actinosynnemataceae was proposed in 2000 has resulted in a markedly different distribution of chemotaxonomic markers within the suborder from that observed at that time. For instance, it is noted that species of the genera Actinokineospora and Allokutzneria contain arabinose in whole-cell hydrolysates, which is not observed in the other genera within the Actinosynnemataceae, and that there are many genera within the family Pseudonocardiaceae as currently described that do not contain arabinose. Phylogenetic analyses of 16S rRNA gene sequences for all taxa within the suborder do not provide any statistical support for the family Actinosynnemataceae, nor are signature nucleotides found that support its continued differentiation from the family Pseudonocardiaceae. The description of the family Pseudonocardiaceae is therefore emended to include the genera previously classified within the family Actinosynnemataceae and the description of the suborder Pseudonocardineae is also emended to reflect this reclassification. C1 [Labeda, D. P.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Goodfellow, M.] Newcastle Univ, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Chun, J.] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea. [Zhi, X. -Y.; Li, W. -J.] Yunnan Univ, Key Lab Microbial Resources, Minist Educ, Kunming 650091, Peoples R China. [Zhi, X. -Y.; Li, W. -J.] Yunnan Univ, Lab Conservat & Utilizat Bioresources, Yunnan Inst Microbiol, Kunming 650091, Peoples R China. RP Labeda, DP (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM David.Labeda@ars.usda.gov NR 44 TC 26 Z9 26 U1 0 U2 2 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUN PY 2011 VL 61 BP 1259 EP 1264 DI 10.1099/ijs.0.024984-0 PN 6 PG 6 WC Microbiology SC Microbiology GA 787AR UT WOS:000292349500004 PM 20601483 ER PT J AU Parton, WJ Del Grosso, SJ Marx, E Swan, AL AF Parton, William J. Del Grosso, Stephen J. Marx, Ernie Swan, Amy L. TI Agriculture's Role in Cutting Greenhouse Gas Emissions SO ISSUES IN SCIENCE AND TECHNOLOGY LA English DT Editorial Material C1 [Parton, William J.; Marx, Ernie; Swan, Amy L.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Del Grosso, Stephen J.] ARS, USDA, Washington, DC USA. RP Parton, WJ (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. EM billp@nrel.colostate.edu; Steve.DelGrosso@ARS.USDA.GOV; emiemarx@gmail.com; swan@nrel.colostate.edu NR 0 TC 1 Z9 1 U1 1 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0748-5492 J9 ISSUES SCI TECHNOL JI Issues Sci. Technol. PD SUM PY 2011 VL 27 IS 4 BP 29 EP 32 PG 4 WC Engineering, Multidisciplinary; Engineering, Industrial; Multidisciplinary Sciences; Social Issues SC Engineering; Science & Technology - Other Topics; Social Issues GA 787AP UT WOS:000292349300021 ER PT J AU Michopoulos, JG Hermanson, JC Iliopoulos, A Lambrakos, SG Furukawa, T AF Michopoulos, John G. Hermanson, John C. Iliopoulos, Athanasios Lambrakos, Samuel G. Furukawa, Tomonari TI Data-Driven Design Optimization for Composite Material Characterization SO JOURNAL OF COMPUTING AND INFORMATION SCIENCE IN ENGINEERING LA English DT Article DE design optimization; material characterization; mechatronic systems; constitutive response; anisotropic materials; polymer matrix composites; multiaxial testing; full-field methods ID VIRTUAL FIELDS METHOD; NEURAL-NETWORKS; ELASTIC CHARACTERIZATION; MATERIAL IDENTIFICATION; CONSTITUTIVE MODEL; MULTIAXIAL TESTS; DISPLACEMENT; STIFFNESSES; SENSITIVITY; PARAMETERS AB The main goal of the present paper is to demonstrate the value of design optimization beyond its use for structural shape determination in the realm of the constitutive characterization of anisotropic material systems such as polymer matrix composites with or without damage. The approaches discussed are based on the availability of massive experimental data representing the excitation and response behavior of specimens tested by automated mechatronic material testing systems capable of applying multiaxial loading. Material constitutive characterization is achieved by minimizing the difference between experimentally measured and analytically computed system responses as described by surface strain and strain energy density fields. Small and large strain formulations based on additive strain energy density decompositions are introduced and utilized for constructing the necessary objective functions and their subsequent minimization. Numerical examples based on both synthetic (for one-dimensional systems) and actual data (for realistic 3D material systems) demonstrate the successful application of design optimization for constitutive characterization. [DOI: 10.1115/1.3595561] C1 [Michopoulos, John G.; Iliopoulos, Athanasios; Lambrakos, Samuel G.] USN, Res Lab, Ctr Computat Mat Sci, Computat Multiphys Syst Lab, Washington, DC 20375 USA. [Hermanson, John C.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Iliopoulos, Athanasios] Sci Applicat Int Corp, Washington, DC 20375 USA. [Furukawa, Tomonari] Virginia Polytech Inst & State Univ, Dept Mech Engn, Computat Multiphys Syst Lab, Danville, VA 24540 USA. RP Michopoulos, JG (reprint author), USN, Res Lab, Ctr Computat Mat Sci, Computat Multiphys Syst Lab, Washington, DC 20375 USA. RI Hermanson, John/K-8106-2015; Michopoulos, John/D-6704-2016 OI Hermanson, John/0000-0002-3325-6665; Michopoulos, John/0000-0001-7004-6838 FU Office of Naval Research; NRL FX The authors acknowledge the support by the Office of Naval Research and by NRL's 6.1 core-program. The authors also express their gratitude to Dr. Phillip Mast for providing us with pioneering inspiration by initiating both the dream and the reality of industrialized material characterization in the sixties and allowed us to stand on his giant shoulders. NR 65 TC 3 Z9 3 U1 2 U2 8 PU ASME-AMER SOC MECHANICAL ENG PI NEW YORK PA THREE PARK AVE, NEW YORK, NY 10016-5990 USA SN 1530-9827 J9 J COMPUT INF SCI ENG JI J. Comput. Inf. Sci. Eng. PD JUN PY 2011 VL 11 IS 2 AR 021009 DI 10.1115/1.3595561 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Manufacturing SC Computer Science; Engineering GA 782FF UT WOS:000291993200010 ER PT J AU Perry, RW AF Perry, Roger W. TI Fidelity of Bats to Forest Sites Revealed From Mist-Netting Recaptures SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE recaptures; banding; evening bat; northern long-eared bat; red bat; Seminole bat; tri-colored bat ID FEMALE EASTERN PIPISTRELLES; LONG-TERM REUSE; TEMPORAL PATTERNS; AMERICAN-SOCIETY; ROOST SELECTION; HABITAT; LANDSCAPE; MAMMALS; SUMMER; MAMMALOGISTS AB Although site fidelity to permanent roost structures by bats is generally known, long-term fidelity to areas such as foraging or drinking sites is unknown. Furthermore, mist-net recaptures of bats over multiple years are rarely reported. Extensive mist-net surveys were conducted over the course of 8 y in the Ouachita National Forest of central Arkansas, United States to investigate long-term site fidelity and recapture rates of individually marked forest bats. Among eight species that were captured, five species were recaptured over spans >= 1 y, including eastern red bats Lasiurus borealis, Seminole bats L. seminolus, evening bats Nycticeius humeralis, tri-colored bats Perimyotis subflavus, and northern long-eared bats Myotis septentrionalis. Some individuals were recaptured multiple times over multiple years, and the maximum span over which an individual was recaptured was 1 y for Seminole bats, 2 y for tri-colored bats, 3 y for evening bats, 4 y for eastern red bats, and 5 y for northern long-eared bats. These results indicate long-term site fidelity by multiple bat species to particular forest sites, primarily pools along small intermittent streams. Proximity to these sites should be considered when conducting management activities. C1 US Forest Serv, USDA, So Res Stn, Hot Springs, AR 71902 USA. RP Perry, RW (reprint author), US Forest Serv, USDA, So Res Stn, POB 1270, Hot Springs, AR 71902 USA. EM rperry03@fs.fed.us FU Ouachita National Forest; Arkansas Game and Fish Commission; Southern Research Station FX Funding was provided by the Ouachita National Forest through the efforts of LD Hedrick, the Arkansas Game and Fish Commission through the efforts of DB Sasse, and the Southern Research Station through efforts of RE Thill. The use of trade or firm names in this publication is for reader information and does not imply endorsement of any product or service by the U. S. Department of Agriculture. NR 30 TC 6 Z9 6 U1 2 U2 35 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2011 VL 2 IS 1 BP 112 EP 116 DI 10.3996/082010-JFWM-030 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 044MX UT WOS:000311625000014 ER PT J AU Cerveny, LK Blahna, DJ Stern, MJ Mortimer, MJ Freeman, JW AF Cerveny, Lee K. Blahna, Dale J. Stern, Marc J. Mortimer, Michael J. Freeman, James W. TI Forest Service Interdisciplinary Teams: Size, Composition, and Leader Characteristics SO JOURNAL OF FORESTRY LA English DT Article DE US Forest Service; National Environmental Policy Act; interdisciplinary teams; recreation ID ENVIRONMENTAL-POLICY ACT; ECOSYSTEM MANAGEMENT; DIVERSIFICATION; EXPERIENCE; GENDER; NEPA AB Interdisciplinary (ID) teams were created by the US Forest Service in response to environmental legislation. In 2008, we surveyed ID team leaders for National Environmental Policy Act (NEPA) analysis of 106 recreation-related projects conducted between 2005 and 2008. Results were compared with current workforce data and previous studies of ID team leadership and composition for NEPA assessments. ID teams were large in size and diverse in composition, with representatives of a broad range of disciplines and functional areas. The composition of ID teams may be changing from traditional natural resource management to more discipline-specific expertise. The role of social scientists and other human dimension specialists remains modest, despite the importance of social science questions inherent in recreation projects. Results reflect changes in agency hiring practices in the last 20 years and raise questions about the interdisciplinary nature of US Forest Service ID teams. C1 [Cerveny, Lee K.; Blahna, Dale J.] US Forest Serv, Pacific NW Res Stn, Pacific Wildand Fire Sci Lab, Seattle, WA 98103 USA. [Stern, Marc J.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Mortimer, Michael J.] Virginia Polytech Inst & State Univ, Coll Nat Resources, Falls Church, VA 22043 USA. [Freeman, James W.] Phase One Consulting Grp, Alexandria, VA 22314 USA. RP Cerveny, LK (reprint author), US Forest Serv, Pacific NW Res Stn, Pacific Wildand Fire Sci Lab, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. EM lcerveny@fs.fed.us; dblahna@fs.fed.us; mjstern@vt.edu; mortimer@vt.edu; jfreeman@phaseonecg.com OI Stern, Marc/0000-0002-0294-8941 FU US Forest Service, Pacific Northwest Research Station FX This project was funded in part by the US Forest Service, Pacific Northwest Research Station. The authors thank Judy Mikowski for her help in preparing this article. NR 30 TC 3 Z9 3 U1 1 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2011 VL 109 IS 4 BP 201 EP 207 PG 7 WC Forestry SC Forestry GA 783FZ UT WOS:000292068200004 ER PT J AU Becker, DR McCaffrey, SM Abbas, D Halvorsen, KE Jakes, P Moseley, C AF Becker, Dennis R. McCaffrey, Sarah M. Abbas, Dalia Halvorsen, Kathleen E. Jakes, Pamela Moseley, Cassandra TI Conventional Wisdoms of Woody Biomass Utilization on Federal Public Lands SO JOURNAL OF FORESTRY LA English DT Article DE biomass utilization; problem framing; markets; policy ID SOUTHWESTERN UNITED-STATES; PROGRAM; TREES; US AB The appeal of biomass utilization grows as the need for wildfire risk reduction, economic development, and renewable energy generation becomes more pressing. However, uncertainty exists regarding the factors necessary to stimulate use. We draw on in-depth interviews with local industry, agency, community, and tribal representatives from 10 study sites on federal public lands across the United States to examine persistent conventional wisdoms about what hinders biomass use. Findings indicate that the conventional wisdoms were reasonably accurate although the degree to which each impeded progress varied. Their interconnectedness also varied depending on local conditions. Supply guarantees, industry presence, transportation, and the value of the biomass were limiting factors to use, whereas agency budgets and staffing, environmental concerns, and partnerships more aggravated the problem than impeded progress. Understanding the scope and consistency of these accepted truths is important for ensuring that management efforts and ensuing policy effectively targets local use challenges. C1 [Becker, Dennis R.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [McCaffrey, Sarah M.] US Forest Serv, No Res Stn, Evanston, IL 60201 USA. [Halvorsen, Kathleen E.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Jakes, Pamela] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Moseley, Cassandra] Univ Oregon, Ecosyst Workforce Program, Inst Sustainable Environm, Eugene, OR 97403 USA. RP Becker, DR (reprint author), Univ Minnesota, Dept Forest Resources, 1530 Cleveland Ave N,115 Green Hall, St Paul, MN 55108 USA. EM drbecker@umn.edu; smccaffrey@fs.fed.us; abbasd@msu.edu; kehalvor@mtu.edu; pjackes@fs.fed.us; cmoseley@uoregon.edu RI Becker, Dennis/E-6249-2017 FU Joint Fire Sciences Program FX This project was funded by the Joint Fire Sciences Program. NR 34 TC 8 Z9 8 U1 2 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2011 VL 109 IS 4 BP 208 EP 218 PG 11 WC Forestry SC Forestry GA 783FZ UT WOS:000292068200005 ER PT J AU Halofsky, JE Peterson, DL Furniss, MJ Joyce, LA Millar, CI Neilson, RP AF Halofsky, Jessica E. Peterson, David L. Furniss, Michael J. Joyce, Linda A. Millar, Constance I. Neilson, Ronald P. TI Workshop Approach for Developing Climate Change Adaptation Strategies and Actions for Natural Resource Management Agencies in the United States SO JOURNAL OF FORESTRY LA English DT Article DE climate change; adaptation; forest management ID FOREST MANAGEMENT; CONSERVATION; FACE AB Concrete ways to adapt to climate change are needed to help land-management agencies take steps to incorporate climate change into management and take advantage of opportunities to balance the negative effects of climate change. Because the development of adaptation tools and strategies is at an early stage, it is important that ideas and strategies are disseminated quickly to advance thinking and practice. Here, we offer an example of a successful workshop, focused on National Forests in the United States, which allowed quick dissemination of ideas and strategies for climate change adaptation in resource management through an interaction between scientists and managers. We share both the process used in the workshop and the outcome of facilitated dialogue at the workshop. By presenting concrete adaptation methods and showing the value of a focused scientist-manager dialogue, we hope to motivate the US Forest Service and other natural resource agencies to emulate our approach and begin the process of adapting to climate change. C1 [Halofsky, Jessica E.] Univ Washington, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. [Peterson, David L.] US Forest Serv, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. [Furniss, Michael J.] US Forest Serv, Pacific NW Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. [Joyce, Linda A.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Millar, Constance I.] US Forest Serv, Pacific SW Res Stn, Sierra Nevada Res Ctr, Albany, CA 94710 USA. [Neilson, Ronald P.] US Forest Serv, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. RP Halofsky, JE (reprint author), Univ Washington, Pacific Wildland Fire Sci Lab, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. EM jhalo@u.washington.edu; peterson@fs.fed.us; mfurniss@fs.fed.us; ljoyce@fs.fed.us; cmillar@fs.fed.us; neilson@fsl.orst.edu NR 38 TC 2 Z9 2 U1 3 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2011 VL 109 IS 4 BP 219 EP 225 PG 7 WC Forestry SC Forestry GA 783FZ UT WOS:000292068200006 ER PT J AU Smith, B Oswalt, SN AF Smith, Brad Oswalt, Sonja N. TI Status and Progress in Large-Scale Assessments of the Productive Capacity of Forest Ecosystems in the United States SO JOURNAL OF FORESTRY LA English DT Article DE forest area; volume; growth; planted forest; timber harvest; nonwood products; annual inventory AB The Montreal Process is a forest sustainability reporting framework adopted by 12 temperate-boreal nations consisting of 7 basic criteria and 64 indicators. This article focuses specifically on criterion 2, maintenance of the productive capacity of forest ecosystems, and its five indicators: area of forestland and net forestland available for timber production; growing stock and annual increment on forest available for timber production; area, percent, and volume in plantations; and annual removals of nonwood forest products (NWFP). We compare historic and current inventory processes, discuss the implications of each, present criterion 2 indicator highlights, and identify gaps in our current knowledge. In general, the productive capacity of US forests is strong. Forest and timberland areas have been stable for over 50 years, net growth continues to exceed removals, growing stock volume is up 51% since 1953, and planted forests continue to increase in area and provide a larger share of the nation's annual wood production. NWFPs continue to be difficult to track but progress has been made. C1 [Smith, Brad] US Forest Serv, Arlington, VA USA. [Oswalt, Sonja N.] US Forest Serv, Knoxville, TN USA. RP Smith, B (reprint author), US Forest Serv, Arlington, VA USA. EM bsmith12@fs.fed.us; soswalt@fs.fed.us NR 24 TC 2 Z9 2 U1 0 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2011 VL 109 IS 4 BP 226 EP 232 PG 7 WC Forestry SC Forestry GA 783FZ UT WOS:000292068200007 ER PT J AU Tarver, MR Schmelz, EA Scharf, ME AF Tarver, Matthew R. Schmelz, Eric A. Scharf, Michael E. TI Soldier caste influences on candidate primer pheromone levels and juvenile hormone-dependent caste differentiation in workers of the termite Reticulitermes flavipes SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Termite; Chemical ecology; Primer pheromone; Terpene; Juvenile hormone; Caste; Cadinene; Cadinenal ID ZOOTERMOPSIS-ANGUSTICOLLIS HAGEN; GENE-EXPRESSION; NEOTENIC REPRODUCTIVES; SPP. ISOPTERA; HONEY-BEES; RHINOTERMITIDAE; IDENTIFICATION; SECRETIONS; COLONIES; INSECT AB Caste systems and the division of labor they make possible are common underlying features of all social insects. Multiple extrinsic factors have been shown to impact caste composition in social insect colonies. Primer pheromones are one type of extrinsic caste-regulatory factor; they are chemical signaling molecules produced by certain colony members to impact developmental physiology of recipient nestmates. However, only limited evidence exists regarding primer pheromones and their actions in eusocial termites. In previous research we identified two soldier-produced terpenes, gamma-cadinene (CAD) and gamma-cadinenal (ALD), as candidate primer pheromones of the lower termite Reticulitermes flavipes. In the present study we tested hypotheses related to CAD and ALD action in recipient individuals. We examined the influences of terminally developed soldier termites on (1) CAD and ALD levels and (2) caste differentiation in developmentally totipotent workers. Our findings show CAD and ALD (respectively) are caste stimulatory and inhibitory components of chemical blends present in soldier heads, ALD levels increase significantly (10.9x) in workers only in the presence of soldiers, and soldiers can reduce developmental-hormone response thresholds of workers, presumably via ALD action. These findings provide novel evidence supporting that CAD and ALD are authentic caste-regulatory primer pheromones in Reticulitermes. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Tarver, Matthew R.; Scharf, Michael E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Tarver, Matthew R.] USDA ARS SRRC, Formosan Subterranean Termite Res Unit, New Orleans, LA USA. [Schmelz, Eric A.] USDA ARS CMAVE, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Scharf, ME (reprint author), Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. EM mscharf@purdue.edu; eric.schmelz@ars.usda.gov; matt.tarver@ars.usda.gov OI Schmelz, Eric/0000-0002-2837-734X FU CSREES-USDA-NRI [2007-35607-17777]; USDA-NIFA-AFRI [2010-65106-20621] FX We gratefully acknowledge Elizabeth Wood for assistance in conducting JH and isolation bioassays, Dr. Weste Osbrink for helpful discussions, and Drs. Mary Cornelius and Michael Haverty for manuscript review and helpful comments. This work was supported by CSREES-USDA-NRI grant No. 2007-35607-17777 and USDA-NIFA-AFRI grant No. 2010-65106-20621 to MES. NR 64 TC 6 Z9 6 U1 3 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD JUN PY 2011 VL 57 IS 6 BP 771 EP 777 DI 10.1016/j.jinsphys.2011.02.015 PG 7 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 783YM UT WOS:000292120800012 PM 21356212 ER PT J AU Khrimian, A Cosse, AA Crook, DJ AF Khrimian, Ashot Cosse, Allard A. Crook, Damon J. TI Absolute Configuration of 7-epi-Sesquithujene SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID BUG ERYSARCORIS-LEWISI; AGGREGATION PHEROMONE; SANDALWOOD OIL; CONSTITUENTS; SANTALACEAE AB 7-epi-Sesquithujene (1) is a bicyclic sesquiterpene isolated from phoebe oil, an essential oil of the Brazilian walnut tree, Phoebe porosa. It is also produced by stressed ash trees and has been shown to elicit strong electrophysiological responses on emerald ash borer, Agrilus planipennis, antennae. In the course of the development of a synthetic 7-epi-sesquithujene lure for field testing against the emerald ash borer, we found that the absolute configuration of this compound had not been determined. We isolated >95% pure 7-epi-sesquithujene from phoebe oil via successive fractionation and conventional and argentation (HPLC) chromatographies. The specific optical rotation of this compound matched that of a synthetic product of known configuration. We also synthesized two other stereoisomers of sesquithujene and developed a chiral GC method to separate all four. Based on the specific rotation, stereoselective syntheses, and chiral GC analyses, 7-epi-sesquithujene present in phoebe oil and white ash was found to have the 2S,6S,7R absolute configuration. C1 [Khrimian, Ashot] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Cosse, Allard A.] ARS, NCAUR, USDA, Peoria, IL 61604 USA. [Crook, Damon J.] Anim & Plant Hlth Inspect Serv, PPQ CPHST, USDA, Otis Lab, Buzzards Bay, MA 02542 USA. RP Khrimian, A (reprint author), ARS, Beltsville Agr Res Ctr, USDA, Bldg 007,Rm 326,BARC W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM ashot.khrimian@ars.usda.gov FU United States Department of Agriculture FX This work was supported by the United States Department of Agriculture. NMR spectra were obtained in the Analytical NMR Service and Research Center at the University of Maryland. HRSM was performed in the High Resolution Mass Spectroscopy Facility at the University of Iowa. We would like to extend our gratitude to Mr. F. Guzman for help in synthesis and analytical measurements. NR 19 TC 6 Z9 6 U1 0 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 J9 J NAT PROD JI J. Nat. Prod. PD JUN PY 2011 VL 74 IS 6 BP 1414 EP 1420 DI 10.1021/np200098z PG 7 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA 781HF UT WOS:000291920600010 PM 21574561 ER PT J AU Bader, TJ Bednarz, JC AF Bader, Troy J. Bednarz, James C. TI PARENTAL CARE AND DIET OF MISSISSIPPI KITES (ICTINIA MISSISSIPPIENSIS) IN EASTERN ARKANSAS SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Mississippi Kite; Ictinia mississippiensis; diet; parental care; video-recording system ID REPRODUCTIVE SUCCESS; SUPPLEMENTAL FOOD; NORTHERN GOSHAWKS; BREEDING SUCCESS; FEEDING ECOLOGY; VIDEO CAMERA; NEST-SITE; SYSTEM; SIZE; NESTLINGS AB Few studies have quantified parental care patterns and diets of Mississippi Kites (Ictinia mississippiensis). Using video-recording systems, we quantified and analyzed nesting Mississippi Kite parental care and food delivery in eastern Arkansas. During the incubation period, male kites appeared to spend more time ((X) over bar = 311.8 min/day, SE = 30.27) on the nest than females ((X) over bar = 249.6 min/day, SE = 30.62), though the difference was not statistically significant. Females spent significantly more time on the nest during the brooding period than did males. Female kites also spent significantly more time shading nestlings than males. The amount of time that females stayed on the nest overnight during the incubation ((X) over bar = 886 min/night) and brooding periods ((X) over bar = 815 min/night) was longer than that of males ((X) over bar = 806 and 739 min/night, respectively). Male kites delivered more food items (58%) than females (42%) and also delivered significantly more vertebrate food items than did females. Insects were the most common type of food items (80%) delivered to nests. Vertebrates made up 5% of the food items, and the remaining 15% could not be identified. Our data indicated differential sex-division in parental contributions by male and female Mississippi Kites, but a similar contribution in terms of overall parental effort. C1 [Bader, Troy J.; Bednarz, James C.] Arkansas State Univ, Dept Biol Sci, Jonesboro, AR 72467 USA. RP Bader, TJ (reprint author), USDA ARS, Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM troybader@hotmail.com FU U.S. Fish and Wildlife Service (USFWS); Arkansas Game and Fish Commission (AGFC) FX This project was primarily funded by the U.S. Fish and Wildlife Service (USFWS) and Arkansas Game and Fish Commission (AGFC) through a State Wildlife Grant. We would like to thank Karen Rowe (AGFC) for all her knowledge, support., and assistance on this project. Further, we thank the USFWS for help on the logistics on this project. We also thank Richard Hines (USFWS) and the staff at the White River National Wildlife Refuge for all the help they provided on this project and also for looking for kites. We especially are grateful to the 2004 and 2005 research technician, Waylon Edwards, for his endless amount of hard work and dedication to this project. We greatly appreciate the assistance in the field and in the lab provided by Jeremy Brown, T.J. Benson, Dick Baxter, Travis Edwards, Jim Rowe, Erika Bader, and Amy Si,. Pierre. Finally, we thank Cheryl Dykstra, Antoni Margalida, Travis Booms, Jennifer Coulson, Jim Parker, and other anonymous reviewers for their helpful comments on earlier versions of this manuscript. NR 42 TC 5 Z9 5 U1 1 U2 13 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 J9 J RAPTOR RES JI J. Raptor Res. PD JUN PY 2011 VL 45 IS 2 BP 109 EP 118 PG 10 WC Ornithology SC Zoology GA 781HJ UT WOS:000291921200001 ER EF