FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Lebow, P Lebow, S Nelson, W AF Lebow, Patricia Lebow, Stan Nelson, William TI Effect of Treatment Pressure on Treatment Quality and Bending Properties of Red Pine Lumber SO FOREST PRODUCTS JOURNAL LA English DT Article ID PRESERVATIVE TREATMENT; SOUTHERN PINE; CCA AB Although higher treatment pressures have the potential to improve preservative penetration, higher pressures may possibly result in greater reduction in mechanical properties. The present study evaluated the effect of treatment pressure on the treatment quality and mechanical properties of red pine (Pinus resinosa Ait.) lumber. End-matched sections of red pine lumber were treated with an ethanolamine copper preservative at pressures of 1,207 kPa (175 psi), 1,379 kPa (200 psi), or 1,551 kPa (225 psi). Preservative uptake and penetration were measured, and small clear specimens were subsequently cut from the specimens for evaluation of bending properties. The average percentage of sapwood penetration increased slightly with increasing pressure, and this difference was statistically significant between the 1,207-kPa (175-psi) and 1,551-kPa (225-psi) pressures. In comparison to untreated specimens, treatment at all pressures caused small reductions in modulus of rupture and work to maximum load. However, there were no significant differences in bending properties between the pressures evaluated, indicating that higher pressures can be used without additional sacrifice of wood properties. These treatments were conducted at ambient temperature, and the findings do not necessarily apply to treatments conducted at elevated temperatures. C1 [Lebow, Patricia; Lebow, Stan; Nelson, William] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. RP Lebow, P (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM plebow@fs.fed.us; slebow@fs.fed.us; wnelson01@fs.fed.us NR 17 TC 0 Z9 0 U1 0 U2 1 PU FOREST PRODUCTS SOC PI MADISON PA 2801 MARSHALL COURT, MADISON, WI 53705-2295 USA SN 0015-7473 J9 FOREST PROD J JI For. Prod. J. PD AUG PY 2010 VL 60 IS 5 BP 447 EP 452 PG 6 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA 704UK UT WOS:000286080900006 ER PT J AU Polyakov, M Wear, DN Huggett, RN AF Polyakov, Maksym Wear, David N. Huggett, Robert N. TI Harvest Choice and Timber Supply Models for Forest Forecasting SO FOREST SCIENCE LA English DT Article DE conditional logit; elasticity; expectations; simulation ID ECONOMETRIC-ANALYSIS; STANDING FOREST; OWNERS; MARKET; PRICE AB Timber supply has traditionally been modeled using aggregate data, whereas individual harvest choices have been shown to be sensitive to the vintage and condition of forest capital stocks. In this article, we build aggregate supply models for four roundwood products in a seven-state region of the US South directly from stand-level harvest choice models applied to detailed forest inventories. These models allow for a more precise accounting of the biological and economic underpinnings of supply and support forecasting of changes in forest inventories with a high degree of detail. Estimation results support use of the approach. The elasticities of softwood and hardwood sawtimber supply, 0.34 and 0.31, respectively, are consistent with the elasticities reported by previous studies. The elasticities of softwood and hardwood pulpwood supply (respectively, 0.062 and 0.025) are much lower than previous studies found for pulpwood supply, and cross-price elasticities indicate a dominant influence of sawtimber markets on pulpwood supply. Results generally indicate complementarity between sawtimber and pulpwood supply in the short run. This approach can provide a means of predicting the supply consequences of exogenous factors that could alter forest inventories, e.g., climate change and invasive species, and support regular updating of supply models as new inventory data are recorded. FOR. SCI. 56(4):344-355. C1 [Polyakov, Maksym] Univ Western Australia, Nedlands, WA 6009, Australia. [Wear, David N.] US Forest Serv, So Res Stn, Res Triangle Pk, NC 27709 USA. RP Polyakov, M (reprint author), Univ Western Australia, Nedlands, WA 6009, Australia. EM maksym.pdyakov@uwa.edu.au; dwear@fs.fed.us; rhuggett@fs.fed.us RI Polyakov, Maksym/G-1523-2010 OI Polyakov, Maksym/0000-0002-0193-6658 NR 31 TC 5 Z9 5 U1 1 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD AUG PY 2010 VL 56 IS 4 BP 344 EP 355 PG 12 WC Forestry SC Forestry GA 641KF UT WOS:000281123400003 ER PT J AU Marcot, BG Ohmann, JL Mellen-McLean, KL Waddell, KL AF Marcot, Bruce G. Ohmann, Janet L. Mellen-McLean, Kim L. Waddell, Karen L. TI Synthesis of Regional Wildlife and Vegetation Field Studies to Guide Management of Standing and Down Dead Trees SO FOREST SCIENCE LA English DT Article DE forest inventory; snags; down wood; tolerance interval; meta-analysis ID HABITAT SELECTION; POWER ANALYSIS; CONSERVATION; OREGON; ENOUGH; CALIFORNIA; INTERVALS; MODELS AB We used novel methods for combining information from wildlife and vegetation field studies to develop guidelines for managing dead wood for wildlife and biodiversity. The DecAID Decayed Wood Adviser presents data on wildlife use of standing and down dead trees (snags and down wood) and summaries Of regional vegetation plot data depicting dead wood conditions, for forests across the Pacific Northwest United States. We combined data on wildlife use by snag diameter and density and by down wood diameter and cover, across studies, using parametric techniques of meta-analysis. We calculated tolerance intervals, which represent the percentage of each species' population that uses particular sizes or amounts of snags and down wood, and rank-ordered the species into cumulative species curves. We combined data on snags and down wood from >16,000 field plots from three regional forest inventories and calculated distribution-free tolerance intervals compatible with those compiled for wildlife to facilitate integrated analysis. We illustrate our methods using an example for one vegetation condition. The statistical summaries in DecAID use a probabilistic approach, which works well in a risk analysis and management framework, rather than a deterministic approach. Our methods may prove useful to others faced with similar problems of combining information across studies in other regions or for other data types. FOR. SCI. 56(4):391-404. C1 [Marcot, Bruce G.; Ohmann, Janet L.; Waddell, Karen L.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Marcot, BG (reprint author), US Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM bmarcot@fs.fed.us; johmann@fs.fed.us; kmellenmclean@fs.fed.us; kwaddell@fs.fed.us NR 50 TC 8 Z9 9 U1 0 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD AUG PY 2010 VL 56 IS 4 BP 391 EP 404 PG 14 WC Forestry SC Forestry GA 641KF UT WOS:000281123400007 ER PT J AU Duane, MV Cohen, WB Campbell, JL Hudiburg, T Turner, DP Weyermann, DL AF Duane, Maureen V. Cohen, Warren B. Campbell, John L. Hudiburg, Tara Turner, David P. Weyermann, Dale L. TI Implications of Alternative Field-Sampling Designs on Landsat-Based Mapping of Stand Age and Carbon Stocks in Oregon Forests SO FOREST SCIENCE LA English DT Article ID CONTERMINOUS UNITED-STATES; NEAREST-NEIGHBOR IMPUTATION; THEMATIC MAPPER DATA; ABOVEGROUND BIOMASS; WESTERN OREGON; BIOPHYSICAL VARIABLES; ECOSYSTEM PRODUCTION; PACIFIC-NORTHWEST; ETM+ DATA; COVER AB Empirical models relating forest attributes to remotely sensed metrics are widespread in the literature and underpin many of our efforts to map forest structure across complex landscapes. In this study we compared empirical models relating Landsat reflectance to forest age across Oregon using two alternate sets of ground data: one from a large (n similar to 1500) systematic forest inventory and another from a smaller set of plots (n < 50) deliberately selected to represent pure conditions along predefined structural gradients. Models built with the smaller set of targeted ground data resulted in lower plot-level mapping error (root mean square error) and higher apparent explanatory power (R(2)) than those built with the larger, more widely distributed inventory data. However, in two of the three ecoregions considered, predictions derived from models built with the smaller ground data set displayed a bias relative to those built with the larger but noisier inventory data. A modeling exercise, wherein mapped forest age was translated into carbon, demonstrated how nonlinear ecological models can magnify these prediction biases over landscapes. From this study, it is clear that for mapping purposes, inventory data are superior to project-specific data sets if those data sets are not representative of the full region over which mapping is to be done. FOR. SCI. 56(4):405-416. C1 [Duane, Maureen V.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Cohen, Warren B.; Weyermann, Dale L.] US Forest Serv, Morgantown, WV 26505 USA. RP Duane, MV (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. EM maureen.duane@oregonstate.edu; wcohen@fs.fed.us; john.campbell@oregonstate.edu; tara.hudiburg@oregonstate.edu; david.turner@oregonstate.edu; dweyermann@fs.fed.us RI Hudiburg, Tara/G-4556-2010 FU Office of Science (BER), US Department of Energy [ER64360, ER64361] FX We acknowledge the Forest Inventory and Analysis (FIA) Research Program of the US Forest Service for making available confidential FIA plot data and Elizabeth LaPoint (FIA Northern Research Station) for providing data support. We also thank Manuela Huso and Lisa Ganio for statistical consulting and guidance. Matt Gregory, Dirk Pflugmacher, and Robert Kennedy provided much needed programming skills, advice. and assistance. Zhiqiang Yang assisted with the conceptualization of the spectral variable distancebg. This research was supported by the Office of Science (BER), US Department of Energy (Grant ER64360 and ER64361). NR 48 TC 19 Z9 19 U1 0 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD AUG PY 2010 VL 56 IS 4 BP 405 EP 416 PG 12 WC Forestry SC Forestry GA 641KF UT WOS:000281123400008 ER PT J AU Clinton, SM Edwards, RT Findlay, SEG AF Clinton, Sandra M. Edwards, Rick T. Findlay, Stuart E. G. TI Exoenzyme activities as indicators of dissolved organic matter composition in the hyporheic zone of a floodplain river SO FRESHWATER BIOLOGY LA English DT Article DE dissolved organic matter; exoenzyme activity; floodplain river; hyporheic; soil patches ID SONORAN DESERT STREAM; ECOSYSTEM METABOLISM; SPATIAL-DISTRIBUTION; RED ALDER; CARBON; FOREST; SEDIMENTS; WATER; EXCHANGE; SURFACE AB P>1. We measured the hyporheic microbial exoenzyme activities in a floodplain river to determine whether dissolved organic matter (DOM) bioavailability varied with overlying riparian vegetation patch structure or position along flowpaths. 2. Particulate organic matter (POM), dissolved organic carbon (DOC), dissolved oxygen (DO), electrical conductivity and temperature were sampled from wells in a riparian terrace on the Queets River, Washington, U.S.A. on 25 March, 15 May, 20 July and 09 October 1999. Dissolved nitrate, ammonium and soluble reactive phosphorus were also collected on 20 July and 09 October 1999. Wells were characterised by their associated overlying vegetation: bare cobble/young alder, mid-aged alder (8-20 years) and old alder/old-growth conifer (25 to > 100 years). POM was analysed for the ash-free dry mass and the activities of eight exoenzymes (alpha-glucosidase, beta-glucosidase, beta -N-acetylglucosaminidase, xylosidase, phosphatase, leucine aminopeptidase, esterase and endopeptidase) using fluorogenic substrates. 3. Exoenzyme activities in the Queets River hyporheic zone indicated the presence of an active microbial community metabolising a diverse array of organic molecules. Individual exoenzyme activity (mean +/- standard error) ranged from 0.507 +/- 0.1547 to 22.8 +/- 5.69 mu mol MUF (g AFDM)-1 h-1, was highly variable among wells and varied seasonally, with the lowest rates occurring in March. Exoenzyme activities were weakly correlated with DO, DOC and inorganic nutrient concentrations. 4. Ratios of leucine aminopeptidase : beta-glucosidase were low in March, May and October and high in July, potentially indicating a switch from polysaccharides to proteins as the dominant component of microbial metabolism. 5. Principal components analysis indicated that there were patch effects and that these effects were strongest in the summer. 6. DOM degradation patterns did not change systematically along hyporheic flowpaths but varied with overlying forest patch type in the Queets River hyporheic zone, suggesting that additional carbon inputs exist. We hypothesise that the most likely input is the downward movement of DOM from overlying riparian soils. Understanding this movement of DOM from soils to subsurface water is essential for understanding both the hyporheic metabolism and the carbon budget of streams and rivers. C1 [Clinton, Sandra M.] Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA. [Edwards, Rick T.] US Forest Serv, Pacific NW Res Stn, USDA, Juneau, AK USA. [Findlay, Stuart E. G.] Cary Inst Ecosyst Studies, Millbrook, NY USA. RP Clinton, SM (reprint author), Univ N Carolina, Dept Biol, 9201 Univ City Blvd, Charlotte, NC 28223 USA. EM sclinto1@uncc.edu RI yang, lixia/D-7815-2011 FU USDA Forest Service Aquatic and Land Interactions PNW Research Station; Andrew W. Mellon Foundation; Water Center, University of Washington FX We thank Mark Frey, Mark Fry, Scott Bechtold, Holly Coe and Tom O'keefe for help in the field. Drs. Robert Naiman and Robert Edmonds (University of Washington) and two anonymous reviewers provided comments on earlier drafts that significantly improved this manuscript. This research was funded by the USDA Forest Service Aquatic and Land Interactions PNW Research Station (to R. Edwards), Andrew W. Mellon Foundation (to R. Naiman), and The Water Center, University of Washington (to S. Clinton). NR 56 TC 10 Z9 10 U1 1 U2 24 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0046-5070 J9 FRESHWATER BIOL JI Freshw. Biol. PD AUG PY 2010 VL 55 IS 8 BP 1603 EP 1615 DI 10.1111/j.1365-2427.2009.02383.x PG 13 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA 623IZ UT WOS:000279734300002 ER PT J AU Pace, ML Hampton, SE Limburg, KE Bennett, EM Cook, EM Davis, AE Grove, JM Kaneshiro, KY LaDeau, SL Likens, GE McKnight, DM Richardson, DC Strayer, DL AF Pace, Michael L. Hampton, Stephanie E. Limburg, Karin E. Bennett, Elena M. Cook, Elizabeth M. Davis, Ann E. Grove, J. Morgan Kaneshiro, Kenneth Y. LaDeau, Shannon L. Likens, Gene E. McKnight, Diane M. Richardson, David C. Strayer, David L. TI Communicating with the public: opportunities and rewards for individual ecologists SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID SOCIAL-CONTRACT; CLIMATE-CHANGE; SCIENCE; SCIENTISTS; INFORMATION; TECHNOLOGY; MANAGEMENT; KNOWLEDGE; ADVOCACY; AMERICA AB Many ecologists are interested in communicating science to the public and addressing societal concerns about environmental issues. Individual ecologists need to consider whether, when, and how this should be done. We propose that public outreach activities can be beneficial for ecologists at all stages of their career. There are diverse opportunities for such involvement, and these can vary enormously in terms of time and expertise required. Trends within the science of ecology, especially research focused on social-ecological systems, are likely to promote increased interactions with stakeholders and policy makers. To be effective in these interactions, ecologists should consider new approaches to communication and be aware of the potential roles scientists can play in public policy debates. Professional ecologists need to engage with non-scientific audiences; a review of such activities should be included in considerations for promotion, recognition, and awards, while also acknowledging variations in the inclinations and abilities of individual scientists. There are, however, few current standards for how much time ecologists should commit to public outreach, how time allocation might change over a career, or how to evaluate the quality of such activities. We ask ecologists to consider ways to evaluate the quality of interactions with the public and how to reward these efforts appropriately. C1 [Pace, Michael L.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA. [Hampton, Stephanie E.] Univ Calif Santa Barbara, Natl Ctr Ecol Anal & Synth, Santa Barbara, CA 93106 USA. [Limburg, Karin E.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Bennett, Elena M.] McGill Univ, Dept Nat Resources, Montreal, PQ, Canada. [Bennett, Elena M.] McGill Univ, McGill Sch Environm, Montreal, PQ, Canada. [Cook, Elizabeth M.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Davis, Ann E.] Marist Coll, Sch Management, Poughkeepsie, NY USA. [Grove, J. Morgan] US Forest Serv, No Res Stn, USDA, S Burlington, VT USA. [Kaneshiro, Kenneth Y.] Univ Hawaii, Ctr Conservat & Res Training, Honolulu, HI 96822 USA. [LaDeau, Shannon L.; Likens, Gene E.; Richardson, David C.; Strayer, David L.] Cary Inst Ecosyst Studies, Millbrook, NY USA. [McKnight, Diane M.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. RP Pace, ML (reprint author), Univ Virginia, Dept Environm Sci, Clark Hall, Charlottesville, VA 22903 USA. EM mlp5fy@virginia.edu RI Strayer, David/H-3788-2011; Bennett, Elena/A-9553-2008; Limburg, Karin/M-8380-2013; OI LaDeau, Shannon/0000-0003-4825-5435; Pace, Michael/0000-0001-5945-6131; MCKNIGHT, DIANE/0000-0002-4171-1533; Hampton, Stephanie/0000-0003-2389-4249 FU National Science Foundation [DEB-0840224]; USDA Forest Service [09-DG-11132650-083]; US Environmental Protection Agency [EP09H000638]; USDA Agriculture and Food Research Initiative [2009-02609, 2010-85101-20517] FX The authors developed the paper from a series of discussions at the 2009 Cary Conference and subsequent interactions. The Cary Conference was supported by grants from the National Science Foundation (grant # DEB-0840224), the USDA Forest Service (grant # 09-DG-11132650-083), the US Environmental Protection Agency (grant #EP09H000638), and the USDA AFRI Program (grants #2009-02609 and #2010-85101-20517). This paper benefitted from the criticisms and suggestions of various reviewers. NR 51 TC 25 Z9 29 U1 7 U2 48 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD AUG PY 2010 VL 8 IS 6 BP 292 EP 298 DI 10.1890/090168 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 638DI UT WOS:000280871100003 ER PT J AU Whitmer, A Ogden, L Lawton, J Sturner, P Groffman, PM Schneider, L Hart, D Halpern, B Schlesinger, W Raciti, S Bettez, N Ortega, S Rustad, L Pickett, STA Killelea, M AF Whitmer, Ali Ogden, Laura Lawton, John Sturner, Pam Groffman, Peter M. Schneider, Laura Hart, David Halpern, Benjamin Schlesinger, William Raciti, Steve Bettez, Neil Ortega, Sonia Rustad, Lindsey Pickett, Steward T. A. Killelea, Mary TI The engaged university: providing a platform for research that transforms society SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID COMMUNICATION; COMMUNITIES; SCIENCE; HEALTH AB Despite a growing recognition that the solutions to current environmental problems will be developed through collaborations between scientists and stakeholders, substantial challenges stifle such cooperation and slow the transfer of knowledge. Challenges occur at several levels, including individual, disciplinary, and institutional. All of these have implications for scholars working at academic and research institutions. Fortunately, creative ideas and tested models exist that provide opportunities for conversation and serious consideration about how such institutions can facilitate the dialogue between scientists and society. C1 [Whitmer, Ali] Georgetown Univ, Washington, DC 20057 USA. [Ogden, Laura] Florida Int Univ, Dept Global & Sociocultural Studies, Miami, FL 33199 USA. [Lawton, John] Royal Commiss Environm Pollut, London, England. [Sturner, Pam] Stanford Univ, Woods Inst Environm, Stanford, CA 94305 USA. [Groffman, Peter M.; Schlesinger, William; Raciti, Steve; Bettez, Neil; Pickett, Steward T. A.] Boston Univ, Dept Geog & Environm, Boston, MA 02215 USA. [Schneider, Laura] Rutgers State Univ, Dept Geog, New Brunswick, NJ 08903 USA. [Hart, David] Univ Maine, Senator George J Mitchell Ctr Environm & Watershe, Orono, ME USA. [Halpern, Benjamin] Natl Ctr Ecol Anal & Synth, Santa Barbara, CA USA. [Ortega, Sonia] Natl Sci Fdn, Arlington, VA 22230 USA. [Rustad, Lindsey] US Forest Serv, USDA, No Res Stn, Durham, NH USA. [Killelea, Mary] NYU, Dept Biol, New York, NY 10003 USA. RP Whitmer, A (reprint author), Georgetown Univ, Washington, DC 20057 USA. EM whitmer@georgetown.edu RI Raciti, Steve/D-3837-2013; Bettez, Neil/I-5672-2012 OI Raciti, Steve/0000-0002-6793-5068; Bettez, Neil/0000-0002-6859-8083 FU NSF [DEB-0840224]; US Department of Agriculture (USDA) Forest Service [09-DG-11132650-083]; US Environmental Protection Agency [EP09H000638]; USDA Agricultural and Food Research Initiative [2009-02609, 2009-36704469] FX The 2009 Cary Conference was supported by grants from the NSF (grant #DEB-0840224), the US Department of Agriculture (USDA) Forest Service (grant #09-DG-11132650-083), the US Environmental Protection Agency (grant #EP09H000638), and the USDA Agricultural and Food Research Initiative Program (grants #2009-02609 and #2009-36704469). NR 32 TC 43 Z9 44 U1 7 U2 34 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD AUG PY 2010 VL 8 IS 6 BP 314 EP 321 DI 10.1890/090241 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 638DI UT WOS:000280871100006 ER PT J AU Pouyat, RV Weathers, KC Hauber, R Lovett, GM Bartuska, A Christenson, L Davis, JLD Findlay, SEG Menninger, H Rosi-Marshall, E Stine, P Lymn, N AF Pouyat, Richard V. Weathers, Kathleen C. Hauber, Rick Lovett, Gary M. Bartuska, Ann Christenson, Lynn Davis, Jana L. D. Findlay, Stuart E. G. Menninger, Holly Rosi-Marshall, Emma Stine, Peter Lymn, Nadine TI The role of federal agencies in the application of scientific knowledge SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID SCIENCE; POLICY; MANAGEMENT; UNCERTAINTY; NORTHWEST AB Environmental and ecological research has long been characterized as operating along a continuum, with "basic" - representing "investigator-initiated" research - at one end and "applied" - representing " mission-initiated" research - at the other. While federal agency science programs ideally occupy points along this continuum, the resulting science has not always been relevant to solving environmental problems. Here, we suggest that environmental problem-solving by federal agencies has been less effective than it might have been, because of a need for more effective, long-term strategic planning, cooperation across agencies, and incentives for scientists to participate in policy development and implementation. We make suggestions about what federal agencies can do to improve the integration of science, policy, and natural resource management. First, we refer to agency examples, using regulatory (acid deposition) and land management (national forest) issues to illustrate how environmental research intersects with policy development and its implementation. Second, we discuss the barriers that inhibit the application of scientific knowledge in developing and implementing policy - from the perspective of a federal agency and of an individual scientist. Finally, we generate a series of specific recommendations, targeted at federal agencies, individual scientists, and decision makers. C1 [Pouyat, Richard V.] US Forest Serv, Res & Dev, Rosslyn, VA USA. [Weathers, Kathleen C.; Lovett, Gary M.; Findlay, Stuart E. G.; Rosi-Marshall, Emma] Cary Inst Ecosyst Studies, Millbrook, NY USA. [Hauber, Rick] US EPA, Clean Air Markets Div, Washington, DC 20460 USA. [Bartuska, Ann] US Forest Serv, Res & Dev, Washington, DC 20250 USA. [Christenson, Lynn] Vassar Coll, Poughkeepsie, NY 12601 USA. [Davis, Jana L. D.] Chesapeake Bay Trust, Annapolis, MD USA. [Menninger, Holly] Cornell Univ, Ithaca, NY USA. [Stine, Peter] US Forest Serv, Pacific SW Res Stn, Davis, CA USA. [Lymn, Nadine] ESA, Washington, DC USA. RP Pouyat, RV (reprint author), US Forest Serv, Res & Dev, Rosslyn, VA USA. EM rpouyat@fs.fed.us RI Lovett, Gary/H-3800-2013; OI Lovett, Gary/0000-0002-8411-8027 FU NSF [DEB-0840224, 0949558]; USDA Forest Service [09-DG-11132650-083]; US EPA [EP09H000638]; USDA Agricultural and Food Research Initiative [2009-02609, 2009-04469] FX The 2009 Cary Conference was supported by grants from the NSF (grant #DEB-0840224 and #0949558), the USDA Forest Service (grant #09-DG-11132650-083), the US EPA (grant #EP09H000638), and the USDA Agricultural and Food Research Initiative Program (grants #2009-02609 and #2009-04469). We thank D Goldston and R Gropp for their contributions to ideas presented in this manuscript and A Elliott for help with graphics. The views expressed do not in any way reflect the views of the USDA Forest Service and the US Environmental Protection Agency. NR 26 TC 10 Z9 10 U1 0 U2 11 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD AUG PY 2010 VL 8 IS 6 BP 322 EP 328 DI 10.1890/090180 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 638DI UT WOS:000280871100007 ER PT J AU Coram, TE Huang, XL Zhan, GM Settles, ML Chen, XM AF Coram, Tristan E. Huang, Xueling Zhan, Gangming Settles, Matthew L. Chen, Xianming TI Meta-analysis of transcripts associated with race-specific resistance to stripe rust in wheat demonstrates common induction of blue copper-binding protein, heat-stress transcription factor, pathogen-induced WIR1A protein, and ent-kaurene synthase transcripts SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Wheat; Stripe rust; Microarray; Gene expression; Resistance ID F-SP TRITICI; PLANT-DISEASE RESISTANCE; FUSARIUM-GRAMINEARUM; GENE-EXPRESSION; OXIDATIVE BURST; DEFENSE; ARABIDOPSIS; BARLEY; PEROXIDASE; INFECTION AB Resistance to stripe rust in wheat is a preferred method of disease prevention. Race-specific all-stage resistance usually provides complete protection; thus an understanding of the molecular control of race-specific resistance is important. To build on previous studies of race-specific resistance controlled by the Yr5 gene, this study reports the construction and use of a custom oligonucleotide microarray to perform a meta-analysis of the transcriptional response involved in race-specific resistance conferred by Yr1, Yr5, Yr7, Yr8, Yr9, Yr10, Yr15, and Yr17. By profiling the response of eight resistance genes in a common background, we identified 28 transcripts significantly involved in the resistance phenotype across all genotypes. The most significant of these were annotated as blue copper-binding protein, heat-stress transcription factor, pathogen-induced WIR1A protein, and ent-kaurene synthase transcripts. Unique transcripts significant in each genotype were also identified, which highlighted some transcriptional events specific to certain genotypes. The approach was effective in narrowing down the list of candidate genes in comparison to studying individual genotypes. Annotation revealed key gene expression events involved in race-specific resistance. The results confirm the activity of known R-gene-mediated pathway race-specific resistance, including an oxidative burst that likely contributes to a hypersensitive response, as well as pathogenesis-related protein expression and activity of the phenylpropanoid pathway. However, several identified transcripts remained unknown and may prove interesting candidates for further characterization. C1 [Coram, Tristan E.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Huang, Xueling; Zhan, Gangming; Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Settles, Matthew L.] Washington State Univ, Dept Mol Biosci, Pullman, WA 99164 USA. [Chen, Xianming] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Huang, Xueling; Zhan, Gangming] NW A&F Univ, Coll Plant Protect, Xianyang, Shaanxi, Peoples R China. RP Coram, TE (reprint author), ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM tristan.coram@ars.usda.gov RI Coram, Tristan/E-8744-2010; Settles, Matthew/F-2031-2011 OI Settles, Matthew/0000-0002-3424-1086 FU US Department of Agriculture, Agricultural Research Service [5348-22000-014-00D]; USDA-ARS; Washington Wheat Commission [13C-3061-3923] FX This research was supported in part by the US Department of Agriculture, Agricultural Research Service (project no. 5348-22000-014-00D), USDA-ARS Postdoctoral Program, and Washington Wheat Commission (project no. 13C-3061-3923). PPNS no. XXXX, Department of Plant Pathology, College of Agricultural, Human, and Natural Resources Research Center, project numbers WNP00823. The authors acknowledge Lisa Orfe and Dr. Douglas Call for printing the microarrays. NR 39 TC 16 Z9 17 U1 2 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD AUG PY 2010 VL 10 IS 3 BP 383 EP 392 DI 10.1007/s10142-009-0148-5 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 629YT UT WOS:000280238200008 PM 19937262 ER PT J AU Widmer, TL AF Widmer, Timothy L. TI Phytophthora kernoviae oospore maturity, germination, and infection SO FUNGAL BIOLOGY LA English DT Article DE Germination; Oospores; Phytophthora kernoviae; Tetrazolium bromide; Viability stain ID F-SP MEDICAGINIS; IN-VITRO; CHEMICAL FACTORS; LIGHT; INFESTANS; RAMORUM; UK; REPRODUCTION; TEMPERATURE; EPIDEMICS AB Limited information is known on the basic biology of the recently described Phytophthora kernoviae that produces homothallic oospores. In this study, different P. kernoviae isolates were used to investigate oospore maturity, germination, and infection. All isolates produced oospores in V8 broth at 20 degrees C in the dark by 6 d. Oospores also formed at 10 and 15 degrees C, but did not form at 25 and 28 degrees C. Continuous light inhibited oospore production of some isolates but had no negative effect on others. Maturation time of the oospores, as noted by germination and staining with tetrazolium bromide, was not much different among the isolates between 2 and 14 weeks. Oospore germination was optimal at 18 and 20 degrees C, and did not occur at 5, 25, and 30 degrees C. Oospore germination under continuous light was higher than in the dark, but individual isolates showed variable results. Rhododendron leaf disks inoculated with oospores and maintained in the dark at 20 degrees C were necrotic after 1 week, while those kept under continuous light did not develop necrosis. The percentage of leaf disks infected with P. kernoviae was lower in the leaves exposed to continuous light (40 %) compared to those kept in the dark (100 %). Published by Elsevier Ltd on behalf of The British Mycological Society. C1 ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Widmer, TL (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. EM tim.widmer@ars.usda.gov NR 36 TC 3 Z9 5 U1 0 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 J9 FUNGAL BIOL-UK JI Fungal Biol. PD AUG PY 2010 VL 114 IS 8 BP 661 EP 668 DI 10.1016/j.funbio.2010.06.001 PG 8 WC Mycology SC Mycology GA 638WG UT WOS:000280928100007 PM 20943177 ER PT J AU Vega, FE Simpkins, A Aime, MC Posada, F Peterson, SW Rehner, SA Infante, F Castillo, A Arnold, AE AF Vega, Fernando E. Simpkins, Ann Aime, M. Catherine Posada, Francisco Peterson, Stephen W. Rehner, Stephen A. Infante, Francisco Castillo, Alfredo Arnold, A. Elizabeth TI Fungal endophyte diversity in coffee plants from Colombia, Hawai'i, Mexico and Puerto Rico SO FUNGAL ECOLOGY LA English DT Article DE Coffea; Coffee; Endophytes; Tropics ID MOLECULAR CHARACTERIZATION; ANALYSIS REVEALS; THEOBROMA-CACAO; RDNA SEQUENCES; LEAVES; PATHOGEN; IDENTIFICATION; BIODIVERSITY; MYCORRHIZAE; SPECIFICITY AB Coffee (Coffea arabica) plant tissues were surface-sterilized and fungal endophytes isolated using standard techniques, followed by DNA extraction and sequencing of the internal transcribed spacer region (ITS). A total of 843 fungal isolates were recovered and sequenced (Colombia, 267; Hawai'i, 393; Mexico, 109; Puerto Rico, 74) yielding 257 unique ITS genotypes (Colombia, 113; Hawaii, 126; Mexico, 32; Puerto Rico, 40). The most abundant taxa were Colletotrichum, Fusarium, Penicillium, and Xylariaceae. Overall, 220 genotypes were detected in only one of the countries sampled; only two genotypes were found in all four countries. Endophytes were also isolated from Coffea canephora, Coffea congensis, Coffea liberica, Coffea macrocarpa, Coffea racemosa, and Coffea stenophylla in Hawai'i. The high biodiversity of fungal endophytes in coffee plants may indicate that most of these are "accidental tourists" with no role in the plant, in contrast to endophytes that could be defined as "influential passengers" and whose role in the plant has been elucidated. This study, the most comprehensive analysis of fungal endophytes associated with a single host species, demonstrates that coffee plants serve as a reservoir for a wide variety of fungal endophytes that can be isolated from various plant tissues, including the seed, and illustrates the different fungal communities encountered by C. arabica in different coffee-growing regions of the world. (C) 2009 Elsevier Ltd and The British Mycological Society. All rights reserved. C1 [Vega, Fernando E.; Simpkins, Ann] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. [Aime, M. Catherine; Rehner, Stephen A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. [Posada, Francisco] Ctr Nacl Invest Cafe, Chinchina, Caldas, Colombia. [Peterson, Stephen W.] ARS, Microbial Genom & Bioproc Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Infante, Francisco; Castillo, Alfredo] El Colegio Frontera ECOSUR, Tapachula 30700, Chiapas, Mexico. [Arnold, A. Elizabeth] Univ Arizona, Dept Plant Sci, Div Plant Pathol & Microbiol, Tucson, AZ 85721 USA. RP Vega, FE (reprint author), ARS, Sustainable Perennial Crops Lab, USDA, Bldg-001,BARC W, Beltsville, MD 20705 USA. EM fernando.vega@ars.usda.gov RI Trejo, Yesenia/D-9257-2012; Alfredo, Castillo/B-5491-2016; OI Alfredo, Castillo/0000-0003-1452-0026; Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 NR 73 TC 59 Z9 64 U1 3 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 J9 FUNGAL ECOL JI Fungal Ecol. PD AUG PY 2010 VL 3 IS 3 BP 122 EP 138 DI 10.1016/j.funeco.2009.07.002 PG 17 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA 619FN UT WOS:000279414700003 ER PT J AU Taskin, H Buyukalaca, S Dogan, HH Rehner, SA O'Donnell, K AF Taskin, Hatira Buyukalaca, Saadet Dogan, Hasan Hueseyin Rehner, Stephen A. O'Donnell, Kerry TI A multigene molecular phylogenetic assessment of true morels (Morchella) in Turkey SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Conservation; EF-1 alpha; GCPSR; ITS rDNA; LSU rDNA; RPB1; RPB2; Species limits ID RIBOSOMAL-RNA GENE; REPRODUCTIVE ISOLATION; SPECIES RECOGNITION; EVOLUTIONARY RELATIONSHIPS; CRYPTIC SPECIATION; MODEL EUKARYOTE; FUNGI; RPB2; SYSTEMATICS; AGARICALES C1 [O'Donnell, Kerry] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Taskin, Hatira; Buyukalaca, Saadet] Cukurova Univ, Dept Hort, Fac Agr, TR-01330 Adana, Turkey. [Dogan, Hasan Hueseyin] Selcuk Univ, Dept Biol, Fac Sci, TR-42079 Konya, Turkey. [Rehner, Stephen A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. RP O'Donnell, K (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM kerry.odonnell@ars.usda.gov FU Scientific and Technological Research Council of Turkey (TUBITAK); Cukurova University [CU-BAP-ZF2009D41] FX Special thanks are due the Scientific and Technological Research Council of Turkey (TUBITAK) and the Cukurova University, Scientific Research Projects Coordinating Office (CU-BAP-ZF2009D41) for supporting the studies of HT at NCAUR, Stacy Sink for excellent technical assistance, Deb Palmquist for assistance with the statistical analyses, Don Fraser for preparation of the publication figures, and Nathane Orwig for running the DNA sequences in the NCAUR DNA core facility. The mention of trade products or firm names does not imply that the US Department of Agriculture recommends them over similar products or other firms not mentioned. NR 53 TC 21 Z9 26 U1 0 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD AUG PY 2010 VL 47 IS 8 BP 672 EP 682 DI 10.1016/j.fgb.2010.05.004 PG 11 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 627LN UT WOS:000280041100002 PM 20580850 ER PT J AU Gwag, JG Dixit, A Park, YJ Ma, KH Kwon, SJ Cho, GT Lee, GA Lee, SY Kang, HK Lee, SH AF Gwag, Jae-Gyun Dixit, Anupam Park, Yong-Jin Ma, Kyung-Ho Kwon, Soon-Jae Cho, Gyu-Taek Lee, Gi-An Lee, Sok-Young Kang, Hee-Kyoung Lee, Suk-Ha TI Assessment of genetic diversity and population structure in mungbean SO GENES & GENOMICS LA English DT Article DE Mungbean; SSRs; Genetic diversity; Population structure ID POLYMERASE CHAIN-REACTION; VIGNA-RADIATA; MICROSATELLITE MARKERS; ARBITRARY PRIMERS; DNA POLYMORPHISMS; WHEAT ACCESSIONS; RAPD ANALYSIS; LINKAGE MAP; AMPLIFICATION; CULTIVARS AB This study was carried out to assess the genetic diversity and to analyze the population genetic structure for a total of 692 mungbean accessions preserved at National Agrobiodiversity Center (NAC) of the Rural Development Administration (RDA), Korea. Mungbean accessions were collected from 27 countries in nine different geographic regions, and were genotyped using 15 microsatellite markers, which were developed in our previous study. A total of 66 alleles were detected among 692 accessions at all the loci with an average of 4.4 alleles per locus. All the microsatellite loci were found to be polymorphic. The expected heterozygosity (H(E)) and polymorphism information content (PIC) ranged from 0.081 to 0.588 (mean = 0.345) and from 0.080 to 0.544 (mean = 0.295), respectively. Of the 66 alleles, 17 (25.8%) were common (frequency range between 0.05 and 0.5), 15 (22.7%) were abundant (frequency range > 0.5), and 34 (51.5%) were rare (frequency range < 0.05). Locus GB-VR-7 provided the highest number of rare alleles(eight), followed by GB-VR-91(six) and GB-VR-113(four). Country-wide comparative study on genetic diversity showed that accessions from the USA possessed the highest genetic diversity (PIC) followed by Nepal, Iran, and Afghanistan. And region-wide showed that accessions from Europe possessed the highest average genetic diversity, followed by accessions from the USA, South Asia, West Asia, and Oceania. Twenty-seven countries were grouped into seven clades by phylogenetic relationship analysis, but clustering pattern did not strictly follow their geographical origin because of extensive germplasm exchange between/among countries and regions. As a result of a model-based analysis (STRUCTURE) of microsatellite data, two distinct genetic groups were identified which shared more than 75% membership with one of the two genetic groups. However the genetic group pattern did not reflect their geographical origin. The Duncan's Multiple Range Test among these two genetic groups and an admixed group, with a mean of 16 phenotypic traits, showed significant difference in 12 quantitative and qualitative traits on the basis of ANOVA. These 15 newly developed SSR markers proved to be useful as DNA markers to detect genetic variation in mungbean germplasm for reasonable management and crossbreeding purposes. C1 [Lee, Suk-Ha] Seoul Natl Univ, Dept Plant Sci, Seoul 151921, South Korea. [Lee, Suk-Ha] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea. [Gwag, Jae-Gyun; Dixit, Anupam; Ma, Kyung-Ho; Kwon, Soon-Jae; Cho, Gyu-Taek; Lee, Gi-An; Lee, Sok-Young] Natl Acad Agr Sci, Natl Agrobiodivers Ctr, RDA, Suwon 441707, Kyunggi, South Korea. [Park, Yong-Jin; Kang, Hee-Kyoung] Kongju Natl Univ, Coll Ind Sci, Yesan 340802, South Korea. [Lee, Suk-Ha] Seoul Natl Univ, Plant Genom & Breeding Inst, Seoul 151921, South Korea. [Kwon, Soon-Jae] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Lee, SH (reprint author), Seoul Natl Univ, Dept Plant Sci, Seoul 151921, South Korea. EM sukhalee@snu.ac.kr FU Agenda Program [20090801-030-001]; Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea; National Academy of Agricultural Science, RDA, Republic of Korea FX This work was supported by the Agenda Program (20090801-030-001), the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and National Academy of Agricultural Science, RDA, Republic of Korea. NR 40 TC 15 Z9 16 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1976-9571 J9 GENES GENOM JI Genes Genom. PD AUG PY 2010 VL 32 IS 4 BP 299 EP 308 DI 10.1007/s13258-010-0014-9 PG 10 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 648CY UT WOS:000281669200002 ER PT J AU Albright, TP Pidgeon, AM Rittenhouse, CD Clayton, MK Flather, CH Culbert, PD Wardlow, BD Radeloff, VC AF Albright, Thomas P. Pidgeon, Anna M. Rittenhouse, Chadwick D. Clayton, Murray K. Flather, Curtis H. Culbert, Patrick D. Wardlow, Brian D. Radeloff, Volker C. TI Effects of drought on avian community structure SO GLOBAL CHANGE BIOLOGY LA English DT Article DE abundance; birds; drought; Great Plains; greenness; mixed effects models; North American Breeding Bird Survey; precipitation; richness; United States ID STANDARDIZED PRECIPITATION INDEX; CLIMATE-CHANGE; POPULATION DECLINES; SPECIES RICHNESS; SATELLITE DATA; TIME-SERIES; BIRDS; VARIABILITY; TRENDS; INFORMATION AB Droughts are expected to become more frequent under global climate change. Avifauna depend on precipitation for hydration, cover, and food. While there are indications that avian communities respond negatively to drought, little is known about the response of birds with differing functional and behavioural traits, what time periods and indicators of drought are most relevant, or how response varies geographically at broad spatial scales. Our goals were thus to determine (1) how avian abundance and species richness are related to drought, (2) whether community variations are more related to vegetation vigour or precipitation deviations and at what time periods relationships were strongest, (3) how response varies among avian guilds, and (4) how response varies among ecoregions with different precipitation regimes. Using mixed effect models and 1989-2005 North American Breeding Bird Survey data over the central United States, we examined the response to 10 precipitation- and greenness-based metrics by abundance and species richness of the avian community overall, and of four behavioural guilds. Drought was associated with the most negative impacts on avifauna in the semiarid Great Plains, while positive responses were observed in montane areas. Our models predict that in the plains, Neotropical migrants respond the most negatively to extreme drought, decreasing by 13.2% and 6.0% in abundance and richness, while permanent resident abundance and richness increase by 11.5% and 3.6%, respectively in montane areas. In most cases, response of abundance was greater than richness and models based on precipitation metrics spanning 32-week time periods were more supported than those covering shorter time periods and those based on greenness. While drought is but one of myriad environmental variations birds encounter, our results indicate that drought is capable of imposing sizable shifts in abundance, richness, and composition on avian communities, an important implication of a more climatically variable future. C1 [Albright, Thomas P.; Pidgeon, Anna M.; Rittenhouse, Chadwick D.; Culbert, Patrick D.; Radeloff, Volker C.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Clayton, Murray K.] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA. [Flather, Curtis H.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. [Wardlow, Brian D.] Univ Nebraska, Natl Drought Mitigat Ctr, Lincoln, NE 68583 USA. RP Albright, TP (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM talbrig1@wisc.edu RI Rittenhouse, Chadwick/G-7169-2012; Radeloff, Volker/B-6124-2016; Flather, Curtis/G-3577-2012 OI Radeloff, Volker/0000-0001-9004-221X; Flather, Curtis/0000-0002-0623-3126 FU NASA FX We gratefully acknowledge support for this research by the NASA Biodiversity Program and the NASA Interdisciplinary Science Program. N. Keuler provided important statistical advice. Colour schemes were based on http://ColorBrewer.org. We thank the R project and the developers of the nlme, GeoR, and gplots modules. Suggestions from J. Pauli, M. Visser, and three anonymous reviewers improved this manuscript. Finally, we thank the many coordinators and volunteers who make BBS possible. NR 63 TC 38 Z9 39 U1 7 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD AUG PY 2010 VL 16 IS 8 BP 2158 EP 2170 DI 10.1111/j.1365-2486.2009.02120.x PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 619QD UT WOS:000279443800002 ER PT J AU Jin, VL Evans, RD AF Jin, V. L. Evans, R. D. TI Microbial 13C utilization patterns via stable isotope probing of phospholipid biomarkers in Mojave Desert soils exposed to ambient and elevated atmospheric CO2 SO GLOBAL CHANGE BIOLOGY LA English DT Article DE 13CO(2); elevated CO2; Mojave Desert; phospholipid fatty acids; pulse-chase ID ACTIVE RHIZOSPHERE MICROORGANISMS; CARBON-DIOXIDE; FATTY-ACIDS; COMMUNITY STRUCTURE; MYCORRHIZAL FUNGI; RESPONSES; ECOSYSTEM; NITROGEN; BACTERIAL; DYNAMICS AB Changes in plant inputs under changing atmospheric CO2 can be expected to alter the size and/or functional characteristics of soil microbial communities which can determine whether soils are a C sink or source. Stable isotope probing was used to trace autotrophically fixed 13C into phospholipid fatty acid (PLFA) biomarkers in Mojave Desert soils planted with the desert shrub, Larrea tridentata. Seedlings were pulse-labeled with 13CO(2) under ambient and elevated CO2 in controlled environmental growth chambers. The label was chased into the soil by extracting soil PLFAs after labeling at Days 0, 2, 10, 24, and 49. Eighteen of 29 PLFAs identified showed 13C enrichment relative to nonlabeled control soils. Patterns of PLFA enrichment varied temporally and were similar for various PLFAs found within a microbial functional group. Enrichment of PLFA 13C generally occurred within the first 2 days in general and fungal biomarkers, followed by increasingly greater enrichment in bacterial biomarkers as the study progressed (Gram-negative, Gram-positive, actinobacteria). While treatment CO2 level did not affect total PLFA-C concentrations, microbial functional group abundances and distribution responded to treatment CO2 level and these shifts persisted throughout the study. Specifically, ratios of bacterial-to-total PLFA-C decreased and fungal-to-bacterial PLFA-C increased under elevated CO2 compared with ambient conditions. Differences in the timing of 13C incorporation into lipid biomarkers coupled with changes in microbial functional groups indicate that microbial community characteristics in Mojave Desert soils have shifted in response to long-term exposure to increased atmospheric CO2. C1 [Jin, V. L.; Evans, R. D.] Washington State Univ, Sch Biol Sci, Lab Biotechnol & Bioanal Stable Isotope Core 2, Pullman, WA 99164 USA. RP Jin, VL (reprint author), Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, East Campus, Lincoln, NE 68583 USA. EM virginia.jin@ars.usda.gov FU US National Science Foundation [NSF-DEB-0424979, NSF-MRI-0421478]; US Dept. of Energy Terrestrial Carbon Processes [DE-FG02-03ER63651] FX The authors acknowledge financial support from the US National Science Foundation (NSF-DEB-0424979 and NSF-MRI-0421478 to RDE) for this research, and the US Dept. of Energy Terrestrial Carbon Processes program (Award No. DE-FG02-03ER63651) for operational support to the NDFF. The authors thank B. Harlow, S. Schaeffer, J. Briggs, J. Schneider, and A. Cho for laboratory and field assistance, and R. Alldredge for statistical consulting. NR 70 TC 25 Z9 26 U1 7 U2 56 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD AUG PY 2010 VL 16 IS 8 BP 2334 EP 2344 DI 10.1111/j.1365-2486.2010.02207.x PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 619QD UT WOS:000279443800015 ER PT J AU Hickman, GC Vanloocke, A Dohleman, FG Bernacchi, CJ AF Hickman, George C. Vanloocke, Andy Dohleman, Frank G. Bernacchi, Carl J. TI A comparison of canopy evapotranspiration for maize and two perennial grasses identified as potential bioenergy crops SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE agriculture; biofuels; evapotranspiration; perennial rhizomatous grasses; water-use efficiency ID AIR CO2 ENRICHMENT; LAND-USE CHANGE; ENERGY-BALANCE; WATER-USE; MISCANTHUS; IMPACTS; CLIMATE; CARBON; WHEAT; US AB In the Midwestern US, perennial rhizomatous grasses (PRGs) are considered one of the most promising vegetation types to be used as a cellulosic feedstock for renewable energy production. The potential widespread use of biomass crops for renewable energy production has sparked numerous environmental concerns, including the impacts of land-use change on the hydrologic cycle. We predicted that total seasonal evapotranspiration (ET) would be higher for PRGs relative to maize resulting from higher leaf area and a prolonged growing season. We further predicted that, compared with maize, higher aboveground biomass associated with PRGs would offset the higher ET and increase water-use efficiency (WUE) in the context of biomass harvests for liquid biofuel production. To test these predictions, ET was estimated during the 2007 growing season for replicated plots of Miscanthus x giganteus (miscanthus), Panicum virgatum (switchgrass), and Zea mays (maize) using a residual energy balance approach. The combination of a 25% higher mean latent heat flux (lambda ET) and a longer growing season resulted in miscanthus having ca. 55% higher cumulative ET over the growing season compared with maize. Cumulative ET for switchgrass was also higher than maize despite similar seasonal-mean lambda ET. Based on total harvested aboveground biomass, WUE was ca. 50% higher for maize relative to miscanthus; however, when WUE calculated from only maize grain biomass was compared with WUE calculated from miscanthus harvested aboveground biomass, this difference disappeared. Although WUE between maize and miscanthus differed postsenescence, there were no differences in incremental WUE throughout the growing season. Despite initial predictions, aboveground biomass for switchgrass was less than maize; thus WUE was substantially lower for switchgrass than for either maize scenario. These results indicate that changes in ET due to large-scale implementation of PRGs in the Midwestern US would likely influence local and regional hydrologic cycles differently than traditional row crops. C1 [Hickman, George C.; Dohleman, Frank G.; Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Vanloocke, Andy] Univ Illinois, Dept Atmospher Sci, Urbana, IL 61801 USA. [Bernacchi, Carl J.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. RP Bernacchi, CJ (reprint author), 193 ERML,1201 W Gregory Dr, Urbana, IL 61801 USA. EM bernacch@illinois.edu RI VanLoocke, Andy/B-1467-2012 FU Energy Biosciences Institute FX The authors wish to thank Don Ort, David Drag, Marcelo Zeri, Ursula Melissa Ruiz Vera, Christina Burke, Sarah Campbell, and Greg McIsaac for helpful comments on the manuscript. We also wish to acknowledge Devin Quarles for assisting with the field measurements. This research was supported by the Energy Biosciences Institute. NR 50 TC 48 Z9 48 U1 3 U2 28 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1757-1693 J9 GCB BIOENERGY JI GCB Bioenergy PD AUG PY 2010 VL 2 IS 4 BP 157 EP 168 DI 10.1111/j.1757-1707.2010.01050.x PG 12 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 624RN UT WOS:000279837900001 ER PT J AU Vanloocke, A Bernacchi, CJ Twine, TE AF Vanloocke, Andy Bernacchi, Carl J. Twine, Tracy E. TI The impacts of Miscanthus x giganteus production on the Midwest US hydrologic cycle SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE Agro-IBIS; biofuels; evapotranspiration; hydrology; land use change; miscanthus; modeling ID MISSISSIPPI RIVER-BASIN; INTEGRATED BIOSPHERE SIMULATOR; CLIMATE MODEL GENESIS; TRANSFER SCHEME LSX; GLOBAL CLIMATE; WATER-BALANCE; LAND-COVER; ENERGY-CONVERSION; CARBON BALANCE; UNITED-STATES AB Perennial grasses are being considered as candidates for biofuel feedstocks to provide an alternative energy source to fossil fuels. Miscanthus x giganteus (miscanthus), in particular, is a grass that is predicted to provide more energy per sown area than corn ethanol and reduce net carbon dioxide emissions by increasing the storage of carbon belowground. Miscanthus uses more water than Zea mays (maize), mainly as a result of a longer growing season and higher productivity. Conversion of current land use for miscanthus production will likely disrupt regional hydrologic cycles, yet the magnitude, timing, and spatial distribution of effects are unknown. Here, we show the effects of five different scenarios of miscanthus production on the simulated Midwest US hydrologic cycle. Given the same historic precipitation observations, our ecosystem model simulation results show that on an annual basis miscanthus uses more water than the ecosystems it will likely replace. The actual timing and magnitude of increased water loss to the atmosphere depends on location; however, substantial increases only occur when miscanthus fraction cover exceeds 25% in dry regions and 50% in nearly all of the Midwest. Our results delineate where large-scale land use conversion to perennial biofuel grasses might deplete soil water resources. Given the fact that some watersheds within the Midwest already have depleted water resources, we expect our results to inform decisions on where to grow perennial grasses for biofuel use to ensure sustainability of energy and water resources, and to minimize the potential for deleterious effects to water quantity and quality. C1 [Twine, Tracy E.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Vanloocke, Andy] Univ Illinois, Dept Atmospher Sci, Urbana, IL 61801 USA. [Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Twine, TE (reprint author), Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle,439 Borlaug Hall, St Paul, MN 55108 USA. EM twine@umn.edu RI VanLoocke, Andy/B-1467-2012; Twine, Tracy/H-2337-2013 OI Twine, Tracy/0000-0002-8457-046X FU Energy Biosciences Institute, University of Illinois at Urbana-Champaign FX We thank Santiago Vianna Cuadra for his help with algorithm development. We also thank Frank Dohleman, Emily Heaton, and George Hickman for supplying data for model validation. Don Ort, Jason Hill, Sarah Davis, Marcelo Zeri, and David Drag helped improve the manuscript. This study was funded by the Energy Biosciences Institute, University of Illinois at Urbana-Champaign. NR 60 TC 80 Z9 84 U1 3 U2 43 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1757-1693 J9 GCB BIOENERGY JI GCB Bioenergy PD AUG PY 2010 VL 2 IS 4 BP 180 EP 191 DI 10.1111/j.1757-1707.2010.01053.x PG 12 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 624RN UT WOS:000279837900003 ER PT J AU Li, XJ Zhou, YD Yan, YL Cai, ZY Feng, F AF Li, Xianjun Zhou, Yongdong Yan, Yonglin Cai, Zhiyong Feng, Fu TI A single cell model for pretreatment of wood by microwave explosion SO HOLZFORSCHUNG LA English DT Article DE microwave explosion; pretreatment; single cell model; wood AB A theoretical model was developed to better understand the process of microwave explosion treatment of wood cells. The cell expansion and critical conditions concerning pressure and temperature of ray parenchyma cells in Eucalyptus urophylla were simulated during microwave pretreatment. The results indicate that longitudinal and circumferential stresses were generated in the cell walls owing to the internal steam pressure during extensive microwave treatment. The circumferential stress is twice as high as the longitudinal stress. The pressure difference reaches its maximum value of 0.84 MPa when the extension ratio is 1.20 for the longitudinal direction and 1.62 for the circumferential direction. The maximum pressure difference at the theoretical yielding point is the critical pressure difference that can eventually rupture the ray cell. The critical pressure difference decreases with increasing cell radius and decreasing shear modulus in the cell wall. This simulated result provides useful information to modify wood at the level of ray parenchyma cells. C1 [Li, Xianjun] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Li, Xianjun; Yan, Yonglin] Cent S Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha, Hunan, Peoples R China. [Zhou, Yongdong; Feng, Fu] Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China. RP Li, XJ (reprint author), US Forest Serv, Forest Prod Lab, USDA, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM lxjmu@yahoo.cn FU National Natural Science Foundation of China [30871983]; State Forestry Administration of China [2006-4-99] FX This work was supported by the National Natural Science Foundation of China (No. 30871983), and the State Forestry Administration of China (2006-4-99). NR 19 TC 9 Z9 11 U1 1 U2 10 PU WALTER DE GRUYTER & CO PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 J9 HOLZFORSCHUNG JI Holzforschung PD AUG PY 2010 VL 64 IS 5 BP 633 EP 637 DI 10.1515/HF.2010.095 PG 5 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA 635AG UT WOS:000280627400013 ER PT J AU Stegmeir, TL Finn, CE Warner, RM Hancock, JF AF Stegmeir, Travis L. Finn, Chad E. Warner, Ryan M. Hancock, James F. TI Performance of an Elite Strawberry Population Derived from Wild Germplasm of Fragaria chiloensis and F. virginiana SO HORTSCIENCE LA English DT Article ID NORTH; GENOTYPES; CULTIVARS AB Fragaria xananassa Duchesne ex Rosier, or the cultivated strawberry, resulted from the hybridization of two wild species, F. chiloensis (L.) Miller and F. virginiana Miller. In an attempt to recreate the cultivated strawberry, elite clones of F. chiloensis and F. virginiana were crossed within species and then hybridized to produce 26 reconstructed populations. Of these populations, FVC11 [(Frederick 9 x LH 50-4) x (Scotts Creek x 2 MAR 1A)] had unusually large fruit size and was selected for further analysis. In the summer of 2008,78 individuals of this population were evaluated for their seasonal flowering patterns, inflorescence number, inflorescence height, crown production, flower number, fruit size, yield, internal color, soluble solids, fruit firmness, and plant vigor. Progeny means were compared with those of the parental means and most traits exhibited transgressive segregation, most notably yield and fruit weight. Significant positive correlations were found between many of the production traits, although there were significant negative correlations between fruit firmness and flower number per inflorescence, fruit firmness and soluble solids, and yield per plant and soluble solids. Overall performance scores were assigned to each genotype by summing their relative performance for each trait in the population. Individuals were identified that combined high values for fruit weight and yield with higher than average values for fruit color, firmness, and soluble solids. Use of this population in breeding programs could help expand the genetic base of the cultivated strawberry with limited linkage drag. C1 [Stegmeir, Travis L.; Warner, Ryan M.; Hancock, James F.] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Finn, Chad E.] USDA ARS, Hort Crops Res Lab, NW Ctr Small Fruit Res, Corvallis, OR 97330 USA. RP Hancock, JF (reprint author), Michigan State Univ, Dept Hort, 342 Plant & Soil Sci Bldg, E Lansing, MI 48824 USA. EM hancock@msu.edu NR 26 TC 10 Z9 10 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 BP 1140 EP 1145 PG 6 WC Horticulture SC Agriculture GA 644OD UT WOS:000281387200001 ER PT J AU Glenn, DM Cooley, N Walker, R Clingeleffer, P Shellie, K AF Glenn, D. Michael Cooley, Nicola Walker, Rob Clingeleffer, Peter Shellie, Krista TI Impact of Kaolin Particle Film and Water Deficit on Wine Grape Water Use Efficiency and Plant Water Relations SO HORTSCIENCE LA English DT Article ID VITIS-VINIFERA L.; CARBON-ISOTOPE DISCRIMINATION; RESTRICTED ROOT VOLUME; PEACH-TREE GROWTH; CABERNET-SAUVIGNON; EMPIRE APPLE; TRANSPIRATION EFFICIENCY; GAS-EXCHANGE; CV SHIRAZ; IRRIGATION AB Water use efficiency (WUE) and response of grape vines (Vitis vinifera L. cvs. 'Cabernet Sauvignon', 'Merlot', and 'Viognier') to a particle film treatment (PFT) under varying levels of applied water were evaluated in Victoria, Australia, and southwestern Idaho. Vines that received the least amount of water had the warmest canopy or leaf surface temperature and the lowest (more negative) leaf water potential, stomata! conductance (g(S)), transpiration (E), and photosynthesis (A). Vines with plus-PFT had cooler leaf and canopy temperature than non-PET vines; however, temperature difference resulting from irrigation was greater than that resulting from PFT. In well-watered vines, particle film application increased leaf water potential and lowered g(S). Point-in-time measurements of WUE (A/E) and g(S) did not consistently correspond with seasonal estimates of WUE based on carbon isotope discrimination of leaf or shoot tissue. The response of vines with particle film to undergo stomatal closure and increase leaf water potential conserved water and enhanced WUE under non-limiting soil moisture conditions and the magnitude of response differed according to cultivar. C1 [Glenn, D. Michael] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Cooley, Nicola; Walker, Rob; Clingeleffer, Peter] CSIRO Plant Ind, Merbein, Vic 3505, Australia. [Shellie, Krista] ARS, USDA, Hort Crops Res Lab, Parma, ID 83660 USA. RP Glenn, DM (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov RI Clingeleffer, Peter/A-7637-2008; Walker, Rob/C-2764-2015 OI Clingeleffer, Peter/0000-0001-6818-3542; FU Cooperative Research Centre for Viticulture; Grape and Wine Research and Development Corporation FX The project was funded in part by the Cooperative Research Centre for Viticulture, and the Grape and Wine Research and Development Corporation. The research was a collaboration between USDA-ARS Kearneysville, WV, and Parma. ID, and CSIRO Plant Industry. NR 79 TC 19 Z9 20 U1 0 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 BP 1178 EP 1187 PG 10 WC Horticulture SC Agriculture GA 644OD UT WOS:000281387200008 ER PT J AU Ehlenfeldt, MK Polashock, JJ Stretch, AW Kramer, M AF Ehlenfeldt, Mark K. Polashock, James J. Stretch, Allan W. Kramer, Matthew TI Ranking Cultivated Blueberry for Mummy Berry Blight and Fruit Infection Incidence Using Resampling and Principal Components Analysis SO HORTSCIENCE LA English DT Article ID MONILINIA-VACCINII-CORYMBOSI; HIGHBUSH BLUEBERRY; RESISTANCE; ROT; DISEASE; SUSCEPTIBILITY; GERMPLASM; MODELS; LENGTH AB Mummy berry disease of blueberry has two distinct phases: a blighting phase that infects emerging shoots and leaves early in the spring and a flower infection phase that ultimately leads to infected (mummified) fruit. Cultivated blueberry (Vaccinium spp.) genotypes that are resistant to one phase are not necessarily resistant to the other phase. The resistance of cultivated blueberry (Vaccinium spp.) genotypes to each phase of the disease is different. A large number of cultivars were screened for resistance to each phase. Cultivar standards (cultivars with well-documented responses to the disease) were used in the screening to evaluate long-term variation and aid comparisons across years. Using nine standards for the blight phase, 125 cultivars were tested and ranked for relative resistance using a ranking system based on resampling and principal component analysis. Similarly, using six standards for the flower/fruit infection stage, 110 blueberry cultivars were tested and ranked for relative resistance. Cultivar rankings show that lowbush cultivars and other types possessing high percentages of lowbush germplasm are generally more resistant to both phases of the disease. Among highbush cultivars, Bluejay is reliably resistant to both phases. Documentation of resistance to each phase will allow selection of cultivars for planting in affected areas and will facilitate the development of breeding strategies to produce cultivars with superior resistance. C1 [Ehlenfeldt, Mark K.; Polashock, James J.; Stretch, Allan W.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Henry A Wallace Agr Res Ctr, Beltsville, MD 20705 USA. [Kramer, Matthew] USDA ARS, Biometr Consulting Serv, Henry A Wallace Agr Res Ctr, Beltsville, MD 20705 USA. RP Ehlenfeldt, MK (reprint author), PE Marucci Ctr Blueberry & Cranberry Res, 125A Lake Oswego Rd, Chatsworth, NJ 08019 USA. EM mark.ehlenfeldt@ars.usda.gov NR 20 TC 5 Z9 5 U1 1 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 BP 1205 EP 1210 PG 6 WC Horticulture SC Agriculture GA 644OD UT WOS:000281387200012 ER PT J AU Miller, SS Tworkoski, T AF Miller, Stephen S. Tworkoski, Thomas TI Blossom Thinning in Apple and Peach with an Essential Oil SO HORTSCIENCE LA English DT Article ID SHOOT GROWTH; FRUIT SIZE; TREES; PHOTOSYNTHESIS; DEFOLIATION; BLOOM AB A series of experiments was conducted with apple (Malus xdontestica) and peach [Prunus persica (L.) Batsch] from 2003 to 2008 to evaluate the flower thinning efficacy of eugenol and a eugenol-based essential oil. Flower thinning effects by hand defoliation and alternative chemical agents were compared with eugenol in different years. Eugenol or the eugenol-based contact herbicide Matran 2 EC (or Matratec AG) produced noticeable phytotoxicity to floral parts and exposed leaf tissue within 15 min to 1 h after application and injury was proportional to rate. At the highest rates (8% and 10%), eugenol resulted in complete burning of all exposed tissue except bark tissue, in which there were no visible signs of injury. Within 3 to 4 weeks of application, phytotoxicity was difficult to observe even at the higher rates of eugenol. In companion experiments, hand defoliation of young leaves at bloom resulted in abscission of young fruitlets in apple, but not in peach, indicating that eugenol may cause thinning by multiple mechanisms. Ammonium thiosulfate (ATS) [49 L.ha(-1) or 6.0% (v/v)] provided thinning in peach and showed little or no phytotoxicity, but the response was inconsistent. ATS was also inconsistent in thinning apple. The thinning response from monocarbamidedihydrogen sulphate (MCDS; Wilthin) at 3.2% (v/v) was inconsistent in peach. At the rate used, MCDS caused some phytotoxicity on peach. Applications of 1% to 2% eugenol appear promising, but good blossom coverage is critical for thinning. Furthermore, eugenol formulations need improvement to ensure uniform coverage for more predictable thinning. C1 [Miller, Stephen S.; Tworkoski, Thomas] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Miller, SS (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM stephen.miller@ars.usda.gov NR 33 TC 9 Z9 9 U1 2 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 BP 1218 EP 1225 PG 8 WC Horticulture SC Agriculture GA 644OD UT WOS:000281387200014 ER PT J AU Porch, TG Smith, JR Beaver, JS Griffiths, PD Canaday, CH AF Porch, Timothy G. Smith, James R. Beaver, James S. Griffiths, Phillip D. Canaday, Craig H. TI TARS-HT1 and TARS-HT2 Heat-tolerant Dry Bean Germplasm SO HORTSCIENCE LA English DT Editorial Material ID PHASEOLUS-VULGARIS L; TEMPERATURE; RESISTANCE C1 [Porch, Timothy G.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Smith, James R.] USDA ARS, JWDSRC, Stoneville, MS 38776 USA. [Beaver, James S.] Univ Puerto Rico, Dept Agroenvironm Sci, Recinto Univ Mayaguez, Mayaguez, PR 00681 USA. [Griffiths, Phillip D.] Cornell Univ, New York State Agr Expt Stn, Dept Hort Sci, Geneva, NY 14456 USA. [Canaday, Craig H.] Univ Tennessee, Dept Entomol & Plant Pathol, Jackson, TN 38301 USA. RP Porch, TG (reprint author), USDA ARS, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM timothy.porch@ars.usda.gov RI Rinaldi2, Carlos/D-4479-2011 NR 11 TC 1 Z9 1 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 BP 1278 EP 1280 PG 3 WC Horticulture SC Agriculture GA 644OD UT WOS:000281387200023 ER PT J AU Al-Kofahi, SD Steele, C VanLeeuwen, D St Hilaire, R AF Al-Kofahi, Salman D. Steele, Caiti VanLeeuwen, Dawn St Hilaire, Rolston TI Mapping Land Cover in Urban Residential Landscapes using Fine Resolution Imagery and Object-oriented Classification SO HORTSCIENCE LA English DT Meeting Abstract C1 [Al-Kofahi, Salman D.; VanLeeuwen, Dawn; St Hilaire, Rolston] New Mexico State Univ, Las Cruces, NM 88003 USA. [Steele, Caiti] ARS, USDA, Las Cruces, NM USA. EM slssls76@yahoo.com; caiti@nmsu.edu; vanleeuw@nmsu.edu; rsthilai@nmsu.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S93 EP S94 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800229 ER PT J AU Albano, JP Owen, J Altland, J Evens, TJ Reed, S Yeager, T AF Albano, Joseph P. Owen, Jim Altland, James Evens, Terence J. Reed, Stewart Yeager, Thomas TI Composted Algae as an Alternative Substrate for Horticultural Crop Production: Chemical and Physical Properties SO HORTSCIENCE LA English DT Meeting Abstract C1 [Albano, Joseph P.; Evens, Terence J.] US Hort Res Lab, Ft Pierce, FL USA. [Owen, Jim] N Willamette Res & Ext Ctr, Aurora, OR USA. [Altland, James] ARS, USDA, Wooster, OH USA. [Reed, Stewart] ARS, USDA, Miami, FL USA. [Yeager, Thomas] Univ Florida, IFAS, Gainesville, FL 32611 USA. EM joseph.albano@ars.usda.gov; jim.owen@oregonstate.edu; james.altland@ars.usda.gov; terence.evens@ars.usda.gov; stewart.reed@ars.usda.gov; yeagert@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S164 EP S165 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800394 ER PT J AU Albrecht, E Stommel, JR Saftner, RA AF Albrecht, Elena Stommel, John R. Saftner, Robert A. TI Variability of Free Sugars, Organic Acids and Capsaicinoids in Capsicum baccatum SO HORTSCIENCE LA English DT Meeting Abstract C1 [Albrecht, Elena] KeyGene Inc, Rockville, MD USA. [Stommel, John R.; Saftner, Robert A.] ARS, USDA, Beltsville, MD USA. EM elena.albrecht@keygene.com; john.stommel@ars.usda.gov; robert.saftner@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S289 EP S290 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800682 ER PT J AU Altland, J AF Altland, James TI Invasive Plant Terminology SO HORTSCIENCE LA English DT Meeting Abstract C1 [Altland, James] ARS, USDA, Wooster, OH USA. EM james.altland@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S29 EP S29 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800069 ER PT J AU Anderson, N Byrne, DH Castro, C Raseira, MDB Boonprakob, U Wang, LR Okie, WR AF Anderson, Natalie Byrne, David H. Castro, Caroline Bassola Raseira, Maria do Carmo Boonprakob, Unaroj Wang, Lirong Okie, W. R. TI Diversity of Low Chill Peaches from Asia, Brazil, Europe, and the USA SO HORTSCIENCE LA English DT Meeting Abstract C1 [Anderson, Natalie; Byrne, David H.] Texas A&M Univ, College Stn, TX USA. [Castro, Caroline; Bassola Raseira, Maria do Carmo] EMBRAPA, Pelotas, RS, Brazil. [Boonprakob, Unaroj] Kasetsart Univ, Nakhon Pathom, Thailand. [Wang, Lirong] Chinese Acad Agr Sci, Zhengzhou, Henan, Peoples R China. [Okie, W. R.] ARS, USDA, Byron, GA USA. EM nanderson@tamu.edu; d-byrne@tamu.edu; caroline@cpact.embrapa.br; bassols@cpact.embrapa.br; agrunb@ku.ac.th; wanglirong@caas.net.cn; william.okie@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S53 EP S53 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800139 ER PT J AU Arora, R Rowland, LJ AF Arora, Rajeev Rowland, Lisa J. TI Adaptive Response of Plants to Freezing Stress: A Physiological Perspective SO HORTSCIENCE LA English DT Meeting Abstract C1 [Arora, Rajeev] Iowa State Univ, Ames, IA USA. [Rowland, Lisa J.] ARS, USDA, Beltsville, MD USA. EM rarora@iastate.edu; jeannine.rowland@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S16 EP S17 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800037 ER PT J AU Aryal, N Nimmakayala, P Islam-Faridi, N Levi, A Vajja, GV Reddy, UK AF Aryal, Nischit Nimmakayala, Padma Islam-Faridi, Nurul Levi, Amnon Vajja, Gopinath Venkata Reddy, Umesh K. TI Molecular and Cytogenetic Characterization of Watermelon using DNA Markers and FISH SO HORTSCIENCE LA English DT Meeting Abstract C1 [Aryal, Nischit; Nimmakayala, Padma; Vajja, Gopinath Venkata; Reddy, Umesh K.] W Virginia State Univ, Institute, WV USA. [Islam-Faridi, Nurul] Texas A&M Univ, College Stn, TX USA. [Levi, Amnon] ARS, USDA, Charleston, SC USA. EM naryal@wvstateu.edu; padma@wvstateu.edu; nfaridi@tamu.edu; alevi@ars.usda.gov; gopinath_vajja@yahoo.com; ureddy@wvstateu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S303 EP S303 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800715 ER PT J AU Bai, JH Imahori, Y Baldwin, EA Brecht, JK AF Bai, Jinhe Imahori, Yoshihiro Baldwin, Elizabeth A. Brecht, Jeff K. TI Influence of Chilling and Heating Stress on Oxidative Parameters and Antioxidant Systems in Tomato SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bai, Jinhe] USDA ARS, Winter Haven, FL USA. [Imahori, Yoshihiro] Osaka Pref Univ, Sch Agr, Osaka 5998531, Japan. [Baldwin, Elizabeth A.] USDA, Winter Haven, FL USA. [Brecht, Jeff K.] Univ Florida, Gainesville, FL USA. EM jinhe.bai@ars.usda.gov; imahori@plant.osakafu-u.ac.jp; Liz.Baldwin@ars.usda.gov; jkbrecht@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S244 EP S244 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800572 ER PT J AU Baldwin, EA Plotto, A Loayza, FE Bai, JH Dea, S Brecht, JK AF Baldwin, Elizabeth A. Plotto, Anne Loayza, Francisco E. Bai, Jinhe Dea, Sharon Brecht, Jeffrey K. TI Evaluation of the Impact of Hot Water Treatment on Flavor Compounds of Fresh Tomatoes SO HORTSCIENCE LA English DT Meeting Abstract C1 [Baldwin, Elizabeth A.] USDA, Winter Haven, FL USA. [Plotto, Anne; Bai, Jinhe; Dea, Sharon] USDA ARS, Winter Haven, FL USA. [Loayza, Francisco E.; Brecht, Jeffrey K.] Univ Florida, Gainesville, FL USA. EM Liz.Baldwin@ars.usda.gov; anne.plotto@ars.usda.gov; francisco.loayza@ufl.edu; jinhe.bai@ars.usda.gov; Sharon.Dea@ars.usda.gov; jkbrecht@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S243 EP S244 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800571 ER PT J AU Bamberg, J del Rio, A AF Bamberg, John del Rio, Alfonso TI Use of Native Potatoes for Research and Breeding SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bamberg, John; del Rio, Alfonso] ARS, USDA, Sturgeon Bay, WI USA. EM John.Bamberg@ars.usda.gov; adelrioc@wisc.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S26 EP S26 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800061 ER PT J AU Bassett, C Glenn, DM Forsline, PL Farrell, RE AF Bassett, Carole Glenn, David Michael Forsline, Philip L. Farrell, Robert E. TI Characterizing Water Use Efficiency (WUE) and Water Deficit Responses in Apple (Malus x domestica and Malus sieversii) SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bassett, Carole] ARS, USDA, Kearneysville, WV USA. [Glenn, David Michael] USDA Appalachian Fruit Res Sta, Kearneysville, WV USA. [Forsline, Philip L.] New York Agr Expt Sta, Geneva, NY USA. [Farrell, Robert E.] Penn State Univ, York, PA USA. EM Carole.Bassett@ARS.USDA.GOV; michael.glenn@ars.usda.gov; philip.forsline@ars.usda.gov; jj@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S17 EP S17 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800038 ER PT J AU Bassett, CL Dardick, C Gasic, K Wisniewski, ME Fisher, K AF Bassett, Carole L. Dardick, Chris Gasic, Ksenija Wisniewski, Michael E. Fisher, Kirsten TI The Peach Dehydrin Family Is Small Relative to All Other Sequenced Plant Genomes SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bassett, Carole L.; Dardick, Chris; Wisniewski, Michael E.] ARS, USDA, Kearneysville, WV USA. [Gasic, Ksenija] Clemson Univ, Clemson, SC USA. [Fisher, Kirsten] Univ Calif Los Angelos, Los Angeles, CA USA. EM Carole.Bassett@ars.usda.gov; Chris.Dardick@ars.usda.gov; kgasic@clemson.edu; Michael.Wisniewski@ars.usda.gov; kfisher2@calstatela.edu NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S120 EP S121 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800291 ER PT J AU Bassil, N Gilmore, B Mockler, T Olmstead, J Brown, A Rowland, LJ AF Bassil, Nahla Gilmore, Barbara Mockler, Todd Olmstead, James Brown, Allan Rowland, Lisa J. TI SNP Mining in Blueberry Using Illumina GAII Sequencing SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bassil, Nahla; Gilmore, Barbara] USDA ARS NCGR, Corvallis, OR USA. [Mockler, Todd] Oregon State Univ, Corvallis, OR 97331 USA. [Olmstead, James] Univ Florida, Gainesville, FL USA. [Brown, Allan] NC State Univ, Kannapolis, NC USA. [Rowland, Lisa J.] USDA ARS, Beltsville, MD USA. EM bassiln@hort.oregonstate.edu; barb.gilmore@ars.usda.gov; tmockler@cgrb.oregonstate.edu; jwolmstead@ufl.edu; allan_brown@ncsu.edu; jeannine.rowland@ars.usda.gov RI Mockler, Todd/L-2609-2013 OI Mockler, Todd/0000-0002-0462-5775 NR 0 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S7 EP S8 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800017 ER PT J AU Bassil, N AF Bassil, Nahla TI Applications of Next-Generation Sequencing to Horticultural Crops SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bassil, Nahla] USDA ARS NCGR, Corvallis, OR USA. EM bassiln@hort.oregonstate.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S6 EP S6 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800012 ER PT J AU Bausher, MG Chellemi, DO AF Bausher, Michael G. Chellemi, Daniel O. TI Effect of Rootknot Nematode on Tomato Rootstocks in Non-fumigated Open Field Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bausher, Michael G.; Chellemi, Daniel O.] ARS, USDA, USHRL, Ft Pierce, FL USA. EM michael.bausher@ars.usda.gov; dchellemi@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S MA 6 BP S112 EP S112 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800270 ER PT J AU Beaulieu, JC Grimm, CC Lloyd, SW Stein, RE AF Beaulieu, John C. Grimm, Casey C. Lloyd, Steven W. Stein, Rebecca E. TI Characterizing Endogenous and Oxidative Low Molecular Weight Flavor/Aroma Compounds in Fresh Squeezed/Blended Pomegranate Juice SO HORTSCIENCE LA English DT Meeting Abstract C1 [Beaulieu, John C.; Grimm, Casey C.; Lloyd, Steven W.; Stein, Rebecca E.] ARS, USDA, New Orleans, LA USA. EM John.Beaulieu@ars.usda.gov; Casey.Grim@ars.usda.gov; Steven.Lloyd@ars.usda.gov; Rebecca.Stein@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S143 EP S143 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800342 ER PT J AU Bell, RL AF Bell, Richard L. TI Pear Psylla Resistance in Breeders Selections SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bell, Richard L.] ARS, USDA, Kearneysville, WV USA. EM richard.bell@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S216 EP S216 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800511 ER PT J AU Bi, GH Scagel, CF AF Bi, Guihong Scagel, Carolyn F. TI Effect of Nitrogen Availability on Mineral Nutrient Uptake and Plant Growth of Container-grown Hydrangeas SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bi, Guihong] Mississippi State Univ, Truck Crops Branch, Crystal Springs, MS USA. [Scagel, Carolyn F.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR USA. EM gb250@msstate.edu; scagelc@onid.orst.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S288 EP S289 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800679 ER PT J AU Bryla, DR AF Bryla, David R. TI Irrigation Management Methods to Manipulate Fruit Quality in Blueberry SO HORTSCIENCE LA English DT Meeting Abstract C1 [Bryla, David R.] ARS, USDA, Corvallis, OR USA. EM brylad@onid.orst.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S49 EP S49 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800129 ER PT J AU Callahan, A Dardick, C Scorza, R AF Callahan, Ann Dardick, Chris Scorza, Ralph TI Utilization of Next-Generation Sequencing for Analyzing Transgenic Insertions in Plum: A Risk Assessment Study SO HORTSCIENCE LA English DT Meeting Abstract C1 [Callahan, Ann; Dardick, Chris; Scorza, Ralph] USDA ARS, Kearneysville, WV USA. EM Ann.Callahan@ars.usda.gov; chris.dardick@ars.usda.gov; ralph.scorza@ars.usda.gov NR 0 TC 1 Z9 1 U1 1 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S6 EP S6 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800013 ER PT J AU Davis, A Perkins-Veazie, P Levi, A King, SR Wehner, T AF Davis, Angela Perkins-Veazie, Penelope Levi, Amnon King, Stephen R. Wehner, Todd TI Breeding for Phytonutrient Content: Examples from Watermelon SO HORTSCIENCE LA English DT Meeting Abstract C1 [Davis, Angela] ARS, USDA, S Cent Ag Res Lab, Lane, OK USA. [Perkins-Veazie, Penelope] NC State Univ, Kannapolis, NC USA. [Levi, Amnon] ARS, USDA, Charleston, SC USA. [King, Stephen R.] Texas A&M Univ, Millican, TX USA. [Wehner, Todd] NC State Univ, Raleigh, NC USA. EM angela.davis@lane-ag.org; penelope_perkins@ncsu.edu; alevi@ars.usda.gov; srking@tamu.edu; todd_wehner@ncsu.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S154 EP S154 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800369 ER PT J AU Dea, S Plotto, A Manthey, J Baldwin, EA McCollum, G AF Dea, Sharon Plotto, Anne Manthey, John Baldwin, Elizabeth A. McCollum, Greg TI Insights Regarding Sensory Evaluation of Bitterness Development in Citrus Juice SO HORTSCIENCE LA English DT Meeting Abstract C1 [Dea, Sharon; Plotto, Anne; Manthey, John] ARS, USDA, Winter Haven, FL USA. [McCollum, Greg] ARS, USDA, USHRL, Ft Pierce, FL USA. EM Sharon.Dea@ars.usda.gov; anne.plotto@ars.usda.gov; John.Manthey@ars.usda.gov; Liz.Baldwin@ars.usda.gov; gmccollum@ushrl.ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S307 EP S308 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800725 ER PT J AU Donnelly, LM Reid, MS Jiang, CZ AF Donnelly, Linda M. Reid, Michael S. Jiang, Cai-Zhong TI Virus-Induced Gene Silencing of a NAC Transcription Factor Alters Flower Morphology and Accelerates Flower Senescence in Petunia SO HORTSCIENCE LA English DT Meeting Abstract C1 [Donnelly, Linda M.; Jiang, Cai-Zhong] ARS, USDA, Davis, CA USA. [Reid, Michael S.] Univ Calif Davis, Davis, CA 95616 USA. EM lmdonnelly@ucdavis.edu; msreid@ucdavis.edu; cjiang@ucdavis.edu NR 0 TC 1 Z9 1 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S161 EP S161 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800385 ER PT J AU Dossett, M Lee, J Finn, CE AF Dossett, Michael Lee, Jungmin Finn, Chad E. TI Variation in Anthocyanin Content of Wild Black Raspberry for Breeding Improved Cultivars SO HORTSCIENCE LA English DT Meeting Abstract C1 [Dossett, Michael] Oregon State Univ, Corvallis, OR 97331 USA. [Lee, Jungmin] ARS, USDA, HCRL, Parma, ID USA. [Finn, Chad E.] ARS, USDA, HCRL, Corvallis, OR USA. EM dossettm@hort.oregonstate.edu; Jungmin.Lee@ars.usda.gov; finnc@hort.oregonstate.edu RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S181 EP S182 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800434 ER PT J AU Dossett, M Finn, CE AF Dossett, Michael Finn, Chad E. TI Phenotypic Diversity of Wild Black Raspberry Germplasm SO HORTSCIENCE LA English DT Meeting Abstract C1 [Dossett, Michael] Oregon State Univ, Corvallis, OR 97331 USA. [Finn, Chad E.] ARS, USDA, HCRL, Corvallis, OR USA. EM dossettm@hort.oregonstate.edu; finnc@hort.oregonstate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S121 EP S121 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800292 ER PT J AU Fare, DC AF Fare, Donna C. TI Irrigation Timing and Emitter Selection Affects Irrigation Efficiency and Plant Growth SO HORTSCIENCE LA English DT Meeting Abstract C1 [Fare, Donna C.] USDA ARS, Mcminnville, TN USA. EM dfare@blomand.net NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S264 EP S265 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800619 ER PT J AU Farnham, M AF Farnham, Mark TI Breeding Vegetables Adapted to High Temperatures: A Case Study with Broccoli SO HORTSCIENCE LA English DT Meeting Abstract C1 [Farnham, Mark] ARS, USDA, Charleston, SC USA. EM mark.farnham@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S18 EP S18 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800042 ER PT J AU Farnham, MW Grusak, MA AF Farnham, Mark W. Grusak, Michael A. TI Mineral Concentration of Broccoli Heads in Relation to Year of Cultivar Release SO HORTSCIENCE LA English DT Meeting Abstract C1 [Farnham, Mark W.] USDA ARS, Charleston, SC USA. [Grusak, Michael A.] USDA ARS, Houston, TX USA. EM mark.farnham@ars.usda.gov; Mike.Grusak@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S282 EP S283 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800663 ER PT J AU Fery, RL Thies, JA AF Fery, Richard L. Thies, Judith A. TI Truhart-NR, a Root-knot Nematode Resistant, Pimento-type Pepper Cultivar SO HORTSCIENCE LA English DT Meeting Abstract C1 [Fery, Richard L.; Thies, Judith A.] ARS, USDA, Charleston, SC USA. EM richard.fery@ars.usda.gov; Judy.Thies@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S185 EP S185 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800443 ER PT J AU Fery, RL Thies, JA AF Fery, Richard L. Thies, Judith A. TI PA-560, a Root-knot Nematode Resistant, Yellow-fruited, Habanero-type Pepper SO HORTSCIENCE LA English DT Meeting Abstract C1 [Fery, Richard L.; Thies, Judith A.] ARS, USDA, Charleston, SC USA. EM richard.fery@ars.usda.gov; Judy.Thies@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S184 EP S185 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800442 ER PT J AU Ford, BL Bai, JH Manthey, JA Baldwin, EA AF Ford, Bryan L. Bai, Jinhe Manthey, John A. Baldwin, Elizabeth A. TI Comparison of Oxidative Enzymes and Redox Active Compounds Between Fresh Squeezed and Processed Orange Juices SO HORTSCIENCE LA English DT Meeting Abstract C1 [Ford, Bryan L.; Bai, Jinhe; Manthey, John A.; Baldwin, Elizabeth A.] ARS, USDA, Winter Haven, FL USA. EM bryan.ford@ars.usda.gov; jinhe.bai@ars.usda.gov; john.manthey@ars.usda.gov; liz.baldwin@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S308 EP S309 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800728 ER PT J AU Funk, PA AF Funk, Paul A. TI Pepper Harvest Mechanization: Past and Present SO HORTSCIENCE LA English DT Meeting Abstract C1 [Funk, Paul A.] ARS, USDA, SW Cotton Ginning Res Lab, Mesilla Pk, NM USA. EM pfunk@nmsu.edu NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S21 EP S21 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800049 ER PT J AU Furman, BJ Robertson, NL Hummer, KE AF Furman, Bonnie J. Robertson, Nancy L. Hummer, Kim E. TI The USDA/ARS Rhubarb Collection SO HORTSCIENCE LA English DT Meeting Abstract C1 [Furman, Bonnie J.; Robertson, Nancy L.] ARS, USDA, Palmer, AK USA. [Hummer, Kim E.] ARS, USDA, Corvallis, OR USA. EM bonnie.furman@ars.usda.gov; nancy.robertson@ars.usda.gov; Kim.Hummer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S182 EP S183 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800437 ER PT J AU Furman, BJ Robertson, NL Hummer, KE AF Furman, Bonnie J. Robertson, Nancy L. Hummer, Kim E. TI New Clonal Emphasis for the Arctic and Sub-arctic Plant Genetic Resources Unit in Palmer, Alaska SO HORTSCIENCE LA English DT Meeting Abstract C1 [Furman, Bonnie J.; Robertson, Nancy L.] ARS, USDA, Palmer, AK USA. [Hummer, Kim E.] ARS, USDA, Corvallis, OR USA. EM bonnie.furman@ars.usda.gov; nancy.robertson@ars.usda.gov; Kim.Hummer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S182 EP S182 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800436 ER PT J AU George, LJ Preece, J AF George, Laurie J. Preece, John TI Efficiencies in Alginate Encapsulation of Vegetative Explants SO HORTSCIENCE LA English DT Meeting Abstract C1 [George, Laurie J.] So Illinois Univ, Carbondale, IL 62901 USA. [Preece, John] ARS, USDA, Davis, CA USA. EM laurie_j_george@hotmail.com; John.Preece@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S192 EP S192 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800460 ER PT J AU Goenaga, R Jenkins, D Irish, B AF Goenaga, Ricardo Jenkins, David Irish, Brian TI The Tropical Fruit Research Program of the USDA-ARS Tropical Agriculture Research Station SO HORTSCIENCE LA English DT Meeting Abstract C1 [Goenaga, Ricardo; Jenkins, David; Irish, Brian] USDA ARS, Mayaguez, PR USA. EM ricardo.goenaga@ars.usda.gov; david.jenkins@ars.usda.gov; brian.irish@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S275 EP S276 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800644 ER PT J AU Goenaga, R Jenkins, D Marrero, A AF Goenaga, Ricardo Jenkins, David Marrero, Angel TI Commercial Potential of Rambutan Cultivars Grown at Two Locations in Puerto Rico SO HORTSCIENCE LA English DT Meeting Abstract C1 [Goenaga, Ricardo; Jenkins, David; Marrero, Angel] USDA ARS, Mayaguez, PR USA. EM ricardo.goenaga@ars.usda.gov; david.jenkins@ars.usda.gov; angel.marrero@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S275 EP S275 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800643 ER PT J AU Goenaga, R Salazar-Garcia, S Osuna-Garcia, JA Vigneault, C Doyon, G Gonzalez-Duran, JL AF Goenaga, Ricardo Salazar-Garcia, Samuel Osuna-Garcia, Jorge A. Vigneault, Clement Doyon, Gilles Gonzalez-Duran, Jose L. TI Mission of PROCINORTE's Tropical and Subtropical Fruit Task Force SO HORTSCIENCE LA English DT Meeting Abstract C1 [Goenaga, Ricardo] USDA ARS, Mayaguez, PR USA. [Salazar-Garcia, Samuel; Osuna-Garcia, Jorge A.; Gonzalez-Duran, Jose L.] INIFAP, Nayarit, Mexico. [Vigneault, Clement; Doyon, Gilles] Agr & Agri Food Canada, St Hyacinthe, PQ, Canada. EM ricardo.goenaga@ars.usda.gov; samuelsalazar@prodigy.net.mx; osunajorgealberto@inifap.gob.mx; Clement.Vigneault@AGR.GC.CA; DoyonG@AGR.GC.CA; ijg2@cornell.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S265 EP S266 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800621 ER PT J AU Grieve, C Suarez, DL AF Grieve, Catherine Suarez, Donald L. TI Growth, Yield, and Ion Relations of Strawberry in Response to Irrigation with Chloride-dominated Waters SO HORTSCIENCE LA English DT Meeting Abstract C1 [Grieve, Catherine; Suarez, Donald L.] USDA ARS, US Salin Lab, Riverside, CA 92501 USA. EM cgrieve@ussl.ars.usda.gov; donald.suarez@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S229 EP S229 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800538 ER PT J AU Hadziabdic, D Fitzpatrick, B Wang, XW Wadl, P Rinehart, T Ownley, BH Windham, M Trigiano, R AF Hadziabdic, Denita Fitzpatrick, Benjamin Wang, Xinwang Wadl, Phillip Rinehart, Tim Ownley, Bonnie H. Windham, Mark Trigiano, Robert TI Genetic Diversity of Flowering Dogwood Maintained Despite Massive Mortality Caused by Dogwood Anthracnose SO HORTSCIENCE LA English DT Meeting Abstract C1 [Hadziabdic, Denita; Fitzpatrick, Benjamin; Wadl, Phillip; Ownley, Bonnie H.; Windham, Mark; Trigiano, Robert] Univ Tennessee, Knoxville, TN USA. [Wang, Xinwang] Texas A&M Univ, Texas AgriLife Res & Extens Ctr, Dallas, TX USA. [Rinehart, Tim] ARS, USDA, Poplarville, MS USA. EM DHADZIAB@UTK.EDU; benfitz@utk.edu; xw-wang@tamu.edu; pwadl@utk.edu; Tim.Rinehart@ars.usda.gov; bownley@utk.edu; mwindham@utk.edu; rtrigian@utk.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S148 EP S148 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800354 ER PT J AU Hellier, B AF Hellier, Barbara TI Collecting in Central Asia: National Plant Germplasm System Plant Explorations SO HORTSCIENCE LA English DT Meeting Abstract C1 [Hellier, Barbara] ARS, USDA, Pullman, WA USA. EM bhellier@wsu.edu NR 0 TC 1 Z9 1 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S25 EP S25 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800058 ER PT J AU Hummel, N Coneva, ED Braswell, J Cline, W Marshall, D Ferrin, D Machtmes, K Roy, H Attaway, D AF Hummel, Natalie Coneva, Elina D. Braswell, John Cline, William Marshall, Donna Ferrin, Don Machtmes, Krisanna Roy, Heli Attaway, Denise TI Development of the "All about Blueberries" Extension Community of Practice (CoP) SO HORTSCIENCE LA English DT Meeting Abstract C1 [Hummel, Natalie; Ferrin, Don; Machtmes, Krisanna; Roy, Heli; Attaway, Denise] LSU AgCtr, Baton Rouge, LA USA. [Coneva, Elina D.] Auburn Univ, Auburn, AL 36849 USA. [Braswell, John] Mississippi State Univ, Poplarville, MS USA. [Cline, William] N Carolina State Univ, Castle Hayne, NC USA. [Marshall, Donna] ARS, USDA, Poplarville, MS USA. EM nhummel@agcenter.lsu.edu; edc0001@auburn.edu; braswell@ext.msstate.edu; bill_cline@ncsu.edu; Donna.Marshall@ars.usda.gov; dferrin@agcenter.lsu.edu; KMachtmes@agcenter.lsu.edu; hroy@agcenter.lsu.edu; dattaway@agcenter.lsu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S80 EP S81 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800201 ER PT J AU Hummer, KE AF Hummer, Kim E. TI From Villous Strawberry Shams to Hairy Huckleberries: The Wild Side of Berry Exploration SO HORTSCIENCE LA English DT Meeting Abstract C1 [Hummer, Kim E.] ARS, USDA, Corvallis, OR USA. EM Kim.Hummer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S25 EP S25 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800059 ER PT J AU Irish, B Goenaga, R Crespo, A Wessel-Beaver, L AF Irish, Brian Goenaga, Ricardo Crespo, Arnelis Wessel-Beaver, Linda TI Characterization of Mamey Sapote [Pouteria sapota (Jacq.) HE Moore & Stearn.] Germplasm at the USDA-ARS Tropical Agriculture Research Station SO HORTSCIENCE LA English DT Meeting Abstract C1 [Irish, Brian] USDA ARS TARS, Mayaguez, PR USA. [Goenaga, Ricardo] USDA ARS, Mayaguez, PR USA. [Crespo, Arnelis; Wessel-Beaver, Linda] Univ Puerto Rico, Mayaguez, PR USA. EM brian.irish@ars.usda.gov; ricardo.goenaga@ars.usda.gov; lwesselbeaver@yahoo.com; lwesselbeaver@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S216 EP S216 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800510 ER PT J AU Jeliazkov, VD Cantrell, C Astatkie, T AF Jeliazkov, Valtcho D. Cantrell, Charles Astatkie, Tess TI Potential Essential Oil Crops for Southeastern US SO HORTSCIENCE LA English DT Meeting Abstract CT Annual Conference of the American-Society-for-Horticultural-Science CY AUG 02-05, 2010 CL Palm Desert, CA SP Amer Soc Hort Sci C1 [Jeliazkov, Valtcho D.] Mississippi State Univ, Verona, MS B2N 5E3 USA. [Cantrell, Charles] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. [Astatkie, Tess] Nova Scotia Agr Coll, Truro, NS, Canada. EM vj40@pss.msstate.edu; clcantr1@olemiss.edu; tastatkie@nsac.ca RI Astatkie, Tess/J-7231-2012 OI Astatkie, Tess/0000-0002-9779-8789 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S61 EP S61 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800154 ER PT J AU Jeliazkov, VD Giblette, J Gardner, Z Cantrell, C Craker, LE AF Jeliazkov, Valtcho D. Giblette, Jean Gardner, Zoe Cantrell, Charles Craker, Lyle E. TI Adaptability of Chinese Medicinals for American Growers SO HORTSCIENCE LA English DT Meeting Abstract C1 [Jeliazkov, Valtcho D.] Mississippi State Univ, Verona, MS USA. [Giblette, Jean] High Falls Garden, Philmont, NY USA. [Gardner, Zoe; Craker, Lyle E.] Univ Massachusetts, Amherst, MA 01003 USA. [Cantrell, Charles] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. EM vj40@pss.msstate.edu; hfg@capital.nct; zoe@psis.umass.edu; cicantr1@olemiss.edu; craker@pssci.umass.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S43 EP S43 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800109 ER PT J AU Jenderek, MM Ambruzs, B Andre, C Laufman, J Ellis, D AF Jenderek, Maria M. Ambruzs, Barbara Andre, Christina Laufman, Julie Ellis, David TI Shoot Tip Cryopreservation of Solanum tuberosum Germplasm SO HORTSCIENCE LA English DT Meeting Abstract C1 [Jenderek, Maria M.; Ambruzs, Barbara; Andre, Christina; Ellis, David] ARS, USDA, NCGRP, Ft Collins, CO USA. [Laufman, Julie] US Natl Forest Serv, USDA, ARS, Ft Collins, CO USA. EM maria.jenderek@ars.usda.gov; barbara.ambruzs@ars.usda.gov; christina.andre@ars.usda.gov; jlaufman@fs.fed.us; david.ellis@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S302 EP S302 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800713 ER PT J AU Jeong, KY Nelson, P Frantz, JM AF Jeong, Ka Yeon Nelson, Paul Frantz, Jonathan M. TI Acidification of Soilless Substrate by 13 Floricultural Crops SO HORTSCIENCE LA English DT Meeting Abstract C1 [Jeong, Ka Yeon; Nelson, Paul] N Carolina State Univ, Raleigh, NC 27695 USA. [Frantz, Jonathan M.] Univ Toledo, USDA, ARS, Toledo, OH 43606 USA. EM kyjeong@ncsu.edu; paul_nelson@ncsu.edu; jonathan.frantz@utoledo.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S299 EP S299 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800706 ER PT J AU Jeong, KY Nelson, P Frantz, JM AF Jeong, Ka Yeon Nelson, Paul Frantz, Jonathan M. TI Dairy Manure Compost Effects on Soilless Substrate EC and Plant Nutrient Uptake SO HORTSCIENCE LA English DT Meeting Abstract C1 [Jeong, Ka Yeon; Nelson, Paul] N Carolina State Univ, Raleigh, NC 27695 USA. [Frantz, Jonathan M.] Univ Toledo, ARS, USDA, Toledo, OH 43606 USA. EM kyjeong@ncsu.edu; paul_nelson@ncsu.edu; jonathan.frantz@utoledo.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S101 EP S101 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800247 ER PT J AU Johnson, WC Langston, DB Torrance, RL MacLean, DD AF Johnson, Wiley C. Langston, David B. Torrance, Reid L. MacLean, Daniel D. TI Integrated Systems of Weed Management in Organic 'Vidalia (R)' Onion SO HORTSCIENCE LA English DT Meeting Abstract C1 [Johnson, Wiley C.] ARS, USDA, Tifton, GA USA. [Langston, David B.; MacLean, Daniel D.] Univ Georgia, Tifton, GA USA. [Torrance, Reid L.] Univ Georgia, Lyons, GA USA. EM Carroll.Johnson@ars.usda.gov; dlangsto@uga.edu; reidt@uga.edu; dmaclean@uga.edu RI MacLean, Dan/E-7138-2012 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S136 EP S137 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800328 ER PT J AU Labate, JA Francis, D McGrath, MT Panthee, D Robertson, LD AF Labate, Joanne A. Francis, David McGrath, Margaret T. Panthee, Dilip Robertson, Larry D. TI Diversity in a Collection of Heirloom Tomato Varieties SO HORTSCIENCE LA English DT Meeting Abstract C1 [Labate, Joanne A.] ARS, USDA, PGRU, Geneva, NY USA. [Francis, David] Ohio State Univ, Wooster, OH USA. [McGrath, Margaret T.] Cornell Univ, Riverhead, NY USA. [Panthee, Dilip] N Carolina State Univ, Mills River, NC USA. EM joanne.labate@ars.usda.gov; francis.77@osu.edu; mtm3@cornell.edu; dilip_panthee@ncsu.edu; lrobertson@pgru.ars.usda.gov RI Francis, David/B-7262-2012 NR 0 TC 1 Z9 1 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S145 EP S145 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800346 ER PT J AU Le, S Cherubin, R Boue, S Bhatnagar, D McLachlan, J Ratnayaka, H AF Le, Steven Cherubin, Ronald Boue, Stephen Bhatnagar, Deepak McLachlan, John Ratnayaka, Harish TI Photosynthetic, Antioxidant, and Anticancer Properties of Soybean under Drought and Mechanical Wounding SO HORTSCIENCE LA English DT Meeting Abstract C1 [Le, Steven; Cherubin, Ronald; Ratnayaka, Harish] Xavier Univ Louisiana, New Orleans, LA USA. [Boue, Stephen; Bhatnagar, Deepak] ARS, USDA, SRRC, New Orleans, LA USA. [McLachlan, John] Tulane Univ, New Orleans, LA 70118 USA. EM sle2@xula.edu; rcherubi@xula.edu; steve.boue@ars.usda.gov; deepak.bhatnagar@ars.usda.gov; jmclach@tulane.edu; hratnaya@xula.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S89 EP S90 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800220 ER PT J AU Liang, YC Reid, MS Jiang, CZ AF Liang, Yin-Chih Reid, Michael S. Jiang, Cai-Zhong TI Controlling Plant Architecture by Manipulation of Gibberellic Acid Signaling SO HORTSCIENCE LA English DT Meeting Abstract C1 [Liang, Yin-Chih; Reid, Michael S.] Univ Calif Davis, Davis, CA 95616 USA. [Jiang, Cai-Zhong] USDA ARS, Davis, CA USA. EM imliang@ucdavis.edu; msreid@ucdavis.edu; cjiang@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S279 EP S279 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800653 ER PT J AU Loayza, FE Plotto, A Baldwin, E Bai, JH Dea, S Brecht, JK AF Loayza, Francisco E. Plotto, Anne Baldwin, Elizabeth Bai, Jinhe Dea, Sharon Brecht, Jeffrey K. TI Evaluation of the Impact of Hot Water Treatment on the Sensory Quality of Fresh Tomatoes in Cold Storage SO HORTSCIENCE LA English DT Meeting Abstract C1 [Loayza, Francisco E.; Brecht, Jeffrey K.] Univ Florida, Gainesville, FL USA. [Plotto, Anne; Baldwin, Elizabeth; Bai, Jinhe; Dea, Sharon] USDA ARS, Winter Haven, FL USA. EM francisco.loayza@ufl.edu; anne.plotto@ars.usda.gov; liz.baldwin@ars.usda.gov; jinhe.bai@ars.usda.gov; Sharon.Dea@ars.usda.gov; jkbrecht@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S242 EP S242 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800568 ER PT J AU Luby, J Finn, CE Gasic, K Iezzoni, A Oraguzie, N Brown, SK Byrne, DH Clark, JR Crisosto, CH Davis, TM Evans, K Gradziel, T Hancock, JF Bassil, NV Fazio, G Main, D Peace, C Weebadde, CK van de Weg, E Yue, CY AF Luby, James Finn, Chad E. Gasic, Ksenija Iezzoni, Amy Oraguzie, Nnadozie Brown, Susan K. Byrne, David H. Clark, John R. Crisosto, Carlos H. Davis, Thomas M. Evans, Kate Gradziel, Thomas Hancock, James F. Bassil, Nahla V. Fazio, Gennaro Main, Doreen Peace, Cameron Weebadde, Cholani Kumari van de Weg, Eric Yue, Chengyan TI Germplasm Sets and Standardized Phenotyping Protocols for Fruit Quality Traits in RosBREED SO HORTSCIENCE LA English DT Meeting Abstract CT Annual Conference of the American-Society-for-Horticultural-Science CY AUG 02-05, 2010 CL Palm Desert, CA SP Amer Soc Hort Sci C1 [Luby, James; Yue, Chengyan] Univ Minnesota, St Paul, MN 55108 USA. [Finn, Chad E.] ARS, HCRL, USDA, Corvallis, OR USA. [Gasic, Ksenija] Clemson Univ, Clemson, SC USA. [Iezzoni, Amy] Michigan State Univ, E Lansing, MI USA. [Oraguzie, Nnadozie] Washington State Univ, Prosser, WA USA. [Brown, Susan K.] Cornell Univ, Geneva, NY USA. [Byrne, David H.] Texas A&M Univ, College Stn, TX USA. [Clark, John R.] Univ Arkansas, Fayetteville, AR 72701 USA. [Crisosto, Carlos H.; Gradziel, Thomas] Univ Calif, Davis, CA USA. [Davis, Thomas M.] Univ New Hampshire, Durham, NH 03824 USA. [Evans, Kate] Washington State Univ TFREC, Wenatchee, WA USA. [Hancock, James F.; Weebadde, Cholani Kumari] Michigan State Univ, E Lansing, MI 48824 USA. [Bassil, Nahla V.] ARS, USDA, Corvallis, OR USA. [Fazio, Gennaro] ARS, USDA, Geneva, NY USA. [Main, Doreen; Peace, Cameron] Washington State Univ, Pullman, WA 99164 USA. [van de Weg, Eric] Plant Res Int, Wageningen, Netherlands. EM lubyx001@umn.edu; finnc@hort.oregonstate.edu; kgasic@clemson.edu; iezzoni@msu.edu; noraguzie@wsu.edu; skb3@cornell.edu; d-byrne@tamu.edu; jrclark@uark.edu; carlos@uckac.edu; tom.davis@unh.edu; kate_evans@wsu.edu; tmgradziel@ucdavis.edu; hancock@msu.edu; bassiln@hort.oregonsiate.edu; gf35@cornell.edu; dorrie@wsu.edu; cpeace@wsu.edu; weebadde@msu.edu; vandeweg@wur.nl; yuechy@umn.edu RI Van de Weg, Eric/G-3225-2011 OI Van de Weg, Eric/0000-0002-9443-5974 NR 0 TC 1 Z9 1 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S55 EP S56 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800142 ER PT J AU Lutz, FG Ponniah, SK Nimmakayala, P Tomason, YR Levi, A Reddy, UK AF Lutz, Frank G. Ponniah, Satish K. Nimmakayala, Padma Tomason, Yan R. Levi, Amnon Reddy, Umesh K. TI Syntenic Analysis of Cucurbit Cultivar Complex using Microsatellite Markers SO HORTSCIENCE LA English DT Meeting Abstract C1 [Lutz, Frank G.; Ponniah, Satish K.; Nimmakayala, Padma; Tomason, Yan R.; Reddy, Umesh K.] W Virginia State Univ, Institute, WV USA. [Levi, Amnon] ARS, USDA, Charleston, SC USA. EM papaloots@hotmail.com; skumar@wvstateu.edu; padma@wvstateu.edu; yantomason75@hotmail.com; alevi@ars.usda.gov; ureddy@wvstateu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S90 EP S90 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800221 ER PT J AU Marble, SC Prior, S Runion, GB Torbert, HA Gilliam, CH Fain, GB AF Marble, S. Christopher Prior, Stephen Runion, G. Brett Torbert, H. Allen Gilliam, Charles H. Fain, Glenn B. TI Determining Media and Species Effects on Soil Carbon Dynamics in the Landscape SO HORTSCIENCE LA English DT Meeting Abstract C1 [Marble, S. Christopher; Runion, G. Brett; Torbert, H. Allen; Gilliam, Charles H.; Fain, Glenn B.] Auburn Univ, Auburn, AL 36849 USA. [Prior, Stephen] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL USA. EM marblsc@auburn.edu; steve.prior@ars.usda.gov; brett.runion@ars.usda.gov; allen.torbert@ars.usda.gov; gillic1@auburn.edu; gbf0002@auburn.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S108 EP S109 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800262 ER PT J AU Matsumoto, TK Nagao, MA Zee, FTP Nishijima, K Keith, LM Wall, M AF Matsumoto, Tracie K. Nagao, Mike A. Zee, Francis T. P. Nishijima, Kate Keith, Lisa M. Wall, Marisa TI Foliar Fertilization as an Alternative to Hand-thinning Fruit in Chlorate-treated Dimocarpus longan Lour. Trees in Hawaii SO HORTSCIENCE LA English DT Meeting Abstract C1 [Matsumoto, Tracie K.; Zee, Francis T. P.; Nishijima, Kate; Keith, Lisa M.; Wall, Marisa] ARS, USDA, PWA, PBARC, Hilo, HI USA. [Nagao, Mike A.] Hawaii State Govt UH CTAHR HI, Hilo, HI USA. EM tmatsumoto@pbarc.ars.usda.gov; mnagao@hawaii.edu; Francis.Zee@ars.usda.gov; kate.nishijima@ars.usda.gov; lisa.keith@ars.usda.gov; marisa.wall@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S135 EP S135 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800325 ER PT J AU McCollum, G Stover, E AF McCollum, Greg Stover, Ed TI First Steps toward Rescuing Las-infected Citrus Germplasm SO HORTSCIENCE LA English DT Meeting Abstract C1 [McCollum, Greg; Stover, Ed] ARS, USDA, USHRL, Ft Pierce, FL USA. EM gmccollum@ushrl.ars.usda.gov; ed.stover@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S119 EP S119 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800288 ER PT J AU McCreight, JD Wintermantel, WM AF McCreight, James D. Wintermantel, William M. TI Resistance in Melon PI 313970 to Cucurbit Yellow Stunting Disorder Virus SO HORTSCIENCE LA English DT Meeting Abstract C1 [McCreight, James D.; Wintermantel, William M.] ARS, USDA, Salinas, CA USA. EM Jim.McCreight@ars.usda.gov; bill.wintermantel@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S154 EP S154 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800370 ER PT J AU Mitcham, EJ Brecht, JK Bruhn, CM Barrett, DM Rouseff, RR Danyluk, MD Harris, LJ Diehl, DC Simonne, A Baldwin, EA Cantwell, MI Cassady, DL Cook, R Crisosto, CH Ebeler, SE Emond, JP Huber, DJ Nunes, C Plotto, A Sargent, SA Schneider, KR Sims, CA Slaughter, DC Suslow, TV Wysocki, AF Thompson, JF Zakharov, F AF Mitcham, Elizabeth J. Brecht, Jeffrey K. Bruhn, Christine M. Barrett, Diane M. Rouseff, Russell R. Danyluk, Michelle D. Harris, Linda J. Diehl, David C. Simonne, Amarat Baldwin, Elizabeth A. Cantwell, Marita I. Cassady, Diana L. Cook, Roberta Crisosto, Carlos H. Ebeler, Susan E. Emond, Jean-Pierre Huber, Donald J. Nunes, Cecilia Plotto, Anne Sargent, Steven A. Schneider, Keith R. Sims, Charles A. Slaughter, David C. Suslow, Trevor V. Wysocki, Allen F. Thompson, James F. Zakharov, Florence TI Increasing Consumption of Specialty Crops by Enhancing Their Quality and Safety SO HORTSCIENCE LA English DT Meeting Abstract C1 [Mitcham, Elizabeth J.; Bruhn, Christine M.; Barrett, Diane M.; Harris, Linda J.; Cantwell, Marita I.; Cassady, Diana L.; Cook, Roberta; Crisosto, Carlos H.; Ebeler, Susan E.; Slaughter, David C.; Suslow, Trevor V.; Thompson, James F.; Zakharov, Florence] Univ Calif Davis, Davis, CA 95616 USA. [Brecht, Jeffrey K.; Diehl, David C.; Simonne, Amarat; Emond, Jean-Pierre; Huber, Donald J.; Nunes, Cecilia; Sargent, Steven A.; Schneider, Keith R.; Sims, Charles A.; Wysocki, Allen F.] Univ Florida, Gainesville, FL USA. [Rouseff, Russell R.; Danyluk, Michelle D.] Univ Florida, Lake Alfred, FL USA. [Baldwin, Elizabeth A.; Plotto, Anne] ARS, USDA, Winter Haven, FL USA. EM ejmitcham@ucdavis.edu; jkbrecht@ufl.edu; cmbruhn@ucdavis.edu; dmbarrett@ucdavis.edu; rrouseff@ufl.edu; mddanyluk@ufl.edu; ljharris@ucdavis.edu; dcdiehl@ufl.edu; asim@ufl.edu; liz.baldwin@ars.usda.gov; micantwell@ucdavis.edu; dlcassady@ucdavis.edu; cook@primal.ucdavis.edu; carlos@uckac.edu; seebeler@ucdavis.edu; jpemond@ufl.edu; djhuber@ufl.edu; cnunes@ifas.ufl.edu; anne.plotto@ars.usda.gov; sasa@ufl.edu; keiths29@ufl.edu; csims@ufl.edu; dcslaughter@ucdavis.edu; tvsuslow@ucdavis.edu; wysocki@ufl.edu; jfthompson@ucdavis.edu; fnegre@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S309 EP S310 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800729 ER PT J AU Mitcham, EJ Brecht, JK Bruhn, CM Barrett, DM Rouseff, RR Danyluk, MD Harris, LJ Diehl, DC Simonne, A Baldwin, EA Cantwell, MI Cassady, DL Cook, R Crisosto, CH Ebeler, SE Emond, JP Huber, DJ Nunes, C Plotto, A Sargent, SA Schneider, KR Sims, CA Slaughter, DC Suslow, TV Wysocki, AF Thompson, JF Zakharov, F AF Mitcham, Elizabeth J. Brecht, Jeffrey K. Bruhn, Christine M. Barrett, Diane M. Rouseff, Russell R. Danyluk, Michelle D. Harris, Linda J. Diehl, David C. Simonne, Amarat Baldwin, Elizabeth A. Cantwell, Marita I. Cassady, Diana L. Cook, Roberta Crisosto, Carlos H. Ebeler, Susan E. Emond, Jean-Pierre Huber, Donald J. Nunes, Cecilia Plotto, Anne Sargent, Steven A. Schneider, Keith R. Sims, Charles A. Slaughter, David C. Suslow, Trevor V. Wysocki, Allen F. Thompson, James F. Zakharov, Florence TI Increasing Consumption of Specialty Crops by Enhancing Their Quality and Safety (SCRI) SO HORTSCIENCE LA English DT Meeting Abstract C1 [Mitcham, Elizabeth J.; Bruhn, Christine M.; Barrett, Diane M.; Harris, Linda J.; Cantwell, Marita I.; Cassady, Diana L.; Cook, Roberta; Crisosto, Carlos H.; Ebeler, Susan E.; Slaughter, David C.; Suslow, Trevor V.; Thompson, James F.; Zakharov, Florence] Univ Calif Davis, Davis, CA 95616 USA. [Huber, Donald J.; Sargent, Steven A.] Univ Florida, IFAS, Lake Alfred, FL USA. [Plotto, Anne] ARS, USDA, Winter Haven, FL USA. EM ejmitcham@ucdavis.edu; jkbrecht@ufl.edu; cmbruhn@ucdavis.edu; dmbarrett@ucdavis.edu; rrouseff@ufl.edu; mddanyluk@ufl.edu; ljharris@ucdavis.edu; dcdiehl@ufl.edu; asim@ufl.edu; Liz.Baldwin@ars.usda.gov; micantwell@ucdavis.edu; dlcassady@ucdavis.edu; cook@primal.ucdavis.edu; carlos@uckac.edu; seebeler@ucdavis.edu; jpemond@ufl.edu; djhuber@ufl.edu; cnunes@ifas.ufl.edu; anne.plotto@ars.usda.gov; sasa@ufl.edu; keiths29@ufl.edu; csims@ufl.edu; dcslaughter@ucdavis.edu; tvsuslow@ucdavis.edu; wysocki@ufl.edu; jfthompson@ucdavis.edu; fnegre@ucdavis.cdu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S203 EP S204 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800485 ER PT J AU Morris, B Chase, CA Cho, AH Koenig, R Morales-Payan, JP AF Morris, Brad Chase, Carlene A. Cho, Alyssa H. Koenig, Rosalie Morales-Payan, J. Pablo TI Principal Component Analysis for Morphological, Seed Reproductive, and Phenology Traits in 16 Sunn Hemp (Crotalaria juncea L.) Accessions SO HORTSCIENCE LA English DT Meeting Abstract C1 [Morris, Brad] ARS, USDA, Griffin, GA USA. [Chase, Carlene A.; Cho, Alyssa H.; Koenig, Rosalie] Univ Florida, Gainesville, FL USA. [Morales-Payan, J. Pablo] Univ Puerto Rico, Mayaguez, PR USA. EM brad.morris@ars.usda.gov; cach@mail.ifas.ufl.edu; alycho@ufl.edu; rlkoenig@ufl.edu; jpmorales@uprm.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S182 EP S182 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800435 ER PT J AU Mou, B Liu, HY Benzen, S Richardson, K AF Mou, Beiquan Liu, Hsing-Yeh Benzen, Sharon Richardson, Kelley TI Beet Necrotic Yellow Vein Virus-A New Threat to Spinach Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Mou, Beiquan; Liu, Hsing-Yeh; Benzen, Sharon; Richardson, Kelley] ARS, USDA, Salinas, CA USA. EM beiquan.mou@ars.usda.gov; HsingYeh.Liu@ARS.USDA.GOV; Sharon.Benzen@ars.usda.gov; Kelley.Richardson@ars.usda NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S112 EP S113 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800271 ER PT J AU Niu, LX Zhang, YL Zhang, QX Jiang, CZ Reid, MS AF Niu, Lixin Zhang, Yanlong Zhang, Qi-Xiang Jiang, Cai-Zhong Reid, Michael S. TI Morphological Characterization of Wild Lilium Species Collected from the Vicinity of the Qin-Ba Mountains, China SO HORTSCIENCE LA English DT Meeting Abstract C1 [Niu, Lixin; Zhang, Yanlong] NW A&F Univ, Yangling, Shaanxi, Peoples R China. [Zhang, Qi-Xiang] Beijing Forestry Univ, Beijing, Peoples R China. [Jiang, Cai-Zhong] ARS, USDA, Davis, CA USA. [Reid, Michael S.] Univ Calif Davis, Davis, CA 95616 USA. EM niulixinqh@yahoo.com.cn; susan64sg@yahoo.com; ninishun@gmail.com; cjiang@ucdavis.edu; msreid@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S160 EP S160 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800382 ER PT J AU Obenland, D Margosan, D Smilanick, J AF Obenland, David Margosan, Dennis Smilanick, Joseph TI Extending the Use of Ultraviolet Light for Fruit Quality Sorting in Citrus Packinghouses SO HORTSCIENCE LA English DT Meeting Abstract C1 [Obenland, David; Margosan, Dennis; Smilanick, Joseph] ARS, USDA, Parlier, CA USA. EM david.obenland@ars.usda.gov; dennis.margosan@ars.usda.gov; joseph.smilanick@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S306 EP S307 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800723 ER PT J AU Ostrom, AK Pasian, CC Frantz, JM Jones, ML AF Ostrom, Aaron K. Pasian, Claudio C. Frantz, Jonathan M. Jones, Michelle L. TI Identifying Optimal Fertility Rates for Sustainable Floriculture Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Ostrom, Aaron K.; Pasian, Claudio C.] Ohio State Univ, Columbus, OH 43210 USA. [Frantz, Jonathan M.] Univ Toledo, ARS, USDA, Toledo, OH 43606 USA. [Jones, Michelle L.] Ohio State Univ, OARDC, Wooster, OH USA. EM ostrom.9@osu.edu; pasian.1@osu.edu; jonathan.frantz@utoledo.edu; jones.1968@osu.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S83 EP S84 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800207 ER PT J AU Owen, JS Zazirska-Gabriel, M Sullivan, DM Altland, JE Albano, JP AF Owen, J. S. Zazirska-Gabriel, M. Sullivan, D. M. Altland, J. E. Albano, J. P. TI Water Use and Growth of Two Woody Taxa Produced in Varying Indigenous Douglas Fir Based Soilless Substrates SO HORTSCIENCE LA English DT Meeting Abstract C1 [Owen, J. S.] N Willamette Res & Ext Ctr, Aurora, CO USA. [Zazirska-Gabriel, M.] Univ Calif Riverside, Parlier, CA USA. [Zazirska-Gabriel, M.] ARS, USDA, Parlier, CA USA. [Sullivan, D. M.] Oregon State Univ, Corvallis, OR 97331 USA. [Altland, J. E.] ARS, USDA, Wooster, OH USA. [Albano, J. P.] US Hort Res Lab, Ft Pierce, FL USA. EM jim.owen@oregonstate.edu; Magdalena.Z.Gabriel@ARS.USDA.GOV; dan.sullivan@oregonstate.edu; james.altland@ars.usda.gov; joseph.albano@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S111 EP S111 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800267 ER PT J AU Owen, JS Stoven, HM Altland, JE Pruett, WJ Klick, J AF Owen, J. S. Stoven, H. M. Altland, J. E. Pruett, W. J. Klick, J. TI Crop Response to Hybrid Poplar Alternative Soilless Substrate Component for Pacific Northwest Ornamental Container Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Owen, J. S.; Stoven, H. M.; Klick, J.] N Willamette Res & Ext Ctr, Aurora, CO USA. [Altland, J. E.] ARS, USDA, Wooster, OH USA. [Pruett, W. J.] Phillips Soil Prod Inc, Canby, OR USA. EM jim.owen@oregonstate.edu; heather.stoven@oregonstate.edu; james.altland@ars.usda.gov; wade@phillipssoil.com; jimmy.klick@oregonstate.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S110 EP S111 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800266 ER PT J AU Parfitt, D Hua, SST Holtz, BA Chan, HM Sarreal, SBL Martin-Duvall, T Braga, M AF Parfitt, Dan Hua, Sui Shen T. Holtz, Brent A. Chan, Helen M. Sarreal, Siov Bouy L. Martin-Duvall, Tome Braga, Mike TI Experiments with Biocontrol of Alternaria alternata SO HORTSCIENCE LA English DT Meeting Abstract C1 [Parfitt, Dan; Chan, Helen M.] Univ Calif Davis, Davis, CA 95616 USA. [Hua, Sui Shen T.; Sarreal, Siov Bouy L.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Holtz, Brent A.] Univ Calif Cooperat Extens, Stockton, CA USA. [Martin-Duvall, Tome] Univ Calif Cooperat Extens, Madera, CA USA. [Braga, Mike] Braga Farms, Madera, CA USA. EM deparfitt@ucdavis.edu; ssth@pw.usda.gov; baholtz@ucdavis.edu; hmchan@ucdavis.edu; siov.sarreal@ars.usda.gov; tmmartinduvall@ucdavis.edu; bragafarms@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S227 EP S227 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800534 ER PT J AU Park, SO Ham, IK Lee, EM Yoo, K Lester, GE Crosby, KM AF Park, Soon Oh Ham, In K. Lee, Eun M. Yoo, Kilsun Lester, Gene E. Crosby, Kevin M. TI Molecular Mapping of QTL for Sugars in Melon SO HORTSCIENCE LA English DT Meeting Abstract C1 [Park, Soon Oh] Texas Agr Expt Stn, Weslaco, TX USA. [Ham, In K.; Lee, Eun M.] Chungnam Agr Res & Extens Serv, Yesan, South Korea. [Yoo, Kilsun] Texas A&M Univ, College Stn, TX USA. [Lester, Gene E.] ARS, USDA, SARC, Weslaco, TX USA. [Crosby, Kevin M.] Texas A&M Univ, Weslaco, TX USA. EM so-park@tamu.edu; haminki@empal.com; haminki@empal.com; k-yoo@tamu.edu; gene.lester@ars.usda.gov; k-crosby@tamu.edu NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S185 EP S186 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800444 ER PT J AU Peace, C Bassil, NV Bink, M Brown, SK Byrne, DH Clark, JR Davis, TM Evans, K Fazio, G Finn, CE Gasic, K Gradziel, T Hancock, JF Luby, J Main, D Oraguzie, N van de Weg, E Wang, DC Xu, KN Iezzoni, A AF Peace, Cameron Bassil, Nahla V. Bink, Marco Brown, Susan K. Byrne, David H. Clark, John R. Davis, Thomas M. Evans, Kate Fazio, Gennaro Finn, Chad E. Gasic, Ksenija Gradziel, Thomas Hancock, James F. Luby, James Main, Doreen Oraguzie, Nnadozie van de Weg, Eric Wang, Dechun Xu, Kenong Iezzoni, Amy TI Rosbreed's Marker-assisted Breeding Pipeline SO HORTSCIENCE LA English DT Meeting Abstract C1 [Peace, Cameron; Main, Doreen] Washington State Univ, Pullman, WA 99164 USA. [Bassil, Nahla V.; Finn, Chad E.] ARS, USDA, HCRL, Corvallis, OR USA. [Bink, Marco; van de Weg, Eric] Plant Res Int, Wageningen, Netherlands. [Brown, Susan K.; Xu, Kenong] Cornell Univ, Geneva, NY USA. [Byrne, David H.] Texas A&M Univ, College Stn, TX USA. [Clark, John R.] Univ Arkansas, Fayetteville, AR 72701 USA. [Davis, Thomas M.] Univ New Hampshire, Durham, NH 03824 USA. [Evans, Kate] Washington State Univ TFREC, Wenatchee, WA USA. [Fazio, Gennaro] ARS, USDA, Geneva, NY USA. [Gasic, Ksenija] Clemson Univ, Clemson, SC USA. [Gradziel, Thomas] Univ Calif, Davis, CA USA. [Hancock, James F.; Wang, Dechun; Iezzoni, Amy] Michigan State Univ, E Lansing, MI 48824 USA. [Luby, James] Univ Minnesota, St Paul, MN 55108 USA. [Oraguzie, Nnadozie] Washington State Univ, Prosser, WA USA. EM cpeace@wsu.edu; bassiln@hort.oregonstate.edu; marco.bink@wur.nl; skb3@cornell.edu; d-byrne@tamu.edu; jrclark@uark.edu; tom.davis@unh.edu; kate_evans@wsu.edu; gf35@cornell.edu; finnc@hort.oregonstate.edu; kgasic@clemson.edu; hancock@msu.edu; lubyx001@umn.edu; dorrie@wsu.edu; noraguzie@wsu.edu; vandeweg@wur.nl; wangdech@msu.edu; kx27@cornell.edu; iezzoni@msu.edu RI Van de Weg, Eric/G-3225-2011 OI Van de Weg, Eric/0000-0002-9443-5974 NR 0 TC 2 Z9 2 U1 0 U2 9 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S54 EP S54 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800140 ER PT J AU Plotto, A Gmitter, F Kerbiriou, P Miyazaki, T Valim, MF Baldwin, EA AF Plotto, Anne Gmitter, Fred Kerbiriou, Pauline Miyazaki, Takayuki Valim, M. Filomena Baldwin, Elizabeth A. TI A Three-year Sensory Fruit Quality Study in a Group of New Tangerine Hybrids SO HORTSCIENCE LA English DT Meeting Abstract C1 [Plotto, Anne] ARS, USDA, Winter Haven, FL USA. [Gmitter, Fred; Miyazaki, Takayuki] Univ Florida, Lake Alfred, FL USA. [Kerbiriou, Pauline] Wageningen Univ, Wageningen, Netherlands. [Valim, M. Filomena] Florida Dept Citrus, Lake Alfred, FL USA. EM anne.plotto@ars.usda.gov; fgg@crec.ifas.ufl.edu; pauline.kerbiriou@wur.nl; tomotaka-12137@hotmail.co.jp; fvalim@citrus.state.fl.us; Liz.Baldwin@ars.usda.gov NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S180 EP S181 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800432 ER PT J AU Prior, S Marble, SC Runion, GB Torbert, HA Gilliam, CH AF Prior, Stephen Marble, S. Christopher Runion, G. Brett Torbert, H. Allen Gilliam, Charles H. TI Implications of Elevated Atmospheric CO2 on Plant Growth and Water Relations SO HORTSCIENCE LA English DT Meeting Abstract CT Annual Conference of the American-Society-for-Horticultural-Science CY AUG 02-05, 2010 CL Palm Desert, CA SP Amer Soc Hort Sci C1 [Prior, Stephen] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL USA. [Marble, S. Christopher; Gilliam, Charles H.] Auburn Univ, Auburn, AL 36849 USA. EM steve.prior@ars.usda.gov; marblsc@auburn.edu; brett.runion@ars.usda.gov; allen.torbert@ars.usda.gov; cgilliam@acesag.auburn.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S14 EP S14 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800030 ER PT J AU Rowland, LJ Alkharouf, N Bassil, NV Beers, L Bell, DJ Buck, EJ Drummond, FA Finn, CE Graham, J Hancock, JF McCallum, S Olmstead, J AF Rowland, Lisa J. Alkharouf, Nadim Bassil, Nahla V. Beers, Lee Bell, Daniel J. Buck, Emily J. Drummond, Francis A. Finn, Chad E. Graham, Julie Hancock, James F. McCallum, Susan Olmstead, James TI Generating Genomic Tools for Blueberry Improvement - An Update of Our Progress SO HORTSCIENCE LA English DT Meeting Abstract C1 [Rowland, Lisa J.; Bell, Daniel J.] ARS, USDA, Beltsville, MD USA. [Alkharouf, Nadim] Towson Univ, Towson, MD USA. [Finn, Chad E.] ARS, USDA, HCRL, Corvallis, OR USA. [Beers, Lee; Drummond, Francis A.] Univ Maine, Orono, ME USA. [Buck, Emily J.] New Zealand Inst Plant & Food Res Ltd, Palmerston North, New Zealand. [Graham, Julie; McCallum, Susan] Scottish Crop Res Inst, Invergowrie, Perth & Kinross, Scotland. [Hancock, James F.] Michigan State Univ, E Lansing, MI 48824 USA. [Olmstead, James] Univ Florida, Gainesville, FL USA. EM jeannine.rowland@ars.usda.gov; nalkharouf@towson.edu; bassiln@hort.oregonstate.edu; Lee_Beers@umit.maine.edu; Daniel_J._Bell@umit.maine.edu; Emily.Buck@plantandfood.co.nz; Frank_Drummond@umit.maine.edu; finnc@hort.oregonstate.edu; Julie.Graham@scri.ac.uk; hancock@msu.edu; Susan.McCallum@scri.ac.uk; jwolmstead@ufl.edu RI Buck, Emily/P-2687-2016 OI Buck, Emily/0000-0002-3292-1694 NR 0 TC 0 Z9 0 U1 0 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S197 EP S197 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800473 ER PT J AU Rudell, D Whitaker, B Mattheis, J Zhu, YM AF Rudell, David Whitaker, Bruce Mattheis, Jim Zhu, Yanmin TI Phytosterol Conjugation in Cold-stored Apple Fruit Is Linked to Oxidative Stress and Ripening SO HORTSCIENCE LA English DT Meeting Abstract C1 [Rudell, David; Mattheis, Jim; Zhu, Yanmin] ARS, USDA, Wenatchee, WA USA. [Whitaker, Bruce] ARS, USDA, Beltsville, MD USA. EM rudell@tfrl.ars.usda.gov; bruce.whitaker@ars.usda.gov; james.mattheis@ars.usda.gov; yanmin.zhu@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S58 EP S58 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800147 ER PT J AU Salazar-Garcia, S Gonzalez-Duran, IJL Cossio-Vargas, LE Tapia-Vargas, LM Goenaga, R AF Salazar-Garcia, Samuel Gonzalez-Duran, Isidro J. L. Cossio-Vargas, Luis E. Tapia-Vargas, Luis M. Goenaga, Ricardo TI 'Hass' Avocado Fruit Nutrient Removal as Affected by Production Conditions SO HORTSCIENCE LA English DT Meeting Abstract C1 [Salazar-Garcia, Samuel; Gonzalez-Duran, Isidro J. L.; Cossio-Vargas, Luis E.; Tapia-Vargas, Luis M.] INIFAP, Santiago Ixcuintla, Nayarit, Mexico. [Goenaga, Ricardo] ARS, USDA, Mayaguez, PR USA. EM samuelsalazar@prodigy.net.mx; gonzalez.joseluis@inifap.gob.mx; cossio.luiseduardo@inifap.gob.mx; tapia.luismario@inifap.gob.mx; ricardo.goenaga@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S166 EP S166 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800397 ER PT J AU Samtani, JB Hanson, BD Gerik, JS Fennimore, SA Weber, JB AF Samtani, Jayesh B. Hanson, Bradley D. Gerik, James S. Fennimore, Steven A. Weber, John B. TI Evaluation and Demonstration of a Mobile Steam Applicator to Disinfest Soil in Field-grown California Strawberry and Flower Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Samtani, Jayesh B.; Fennimore, Steven A.; Weber, John B.] Univ Calif Davis, Salinas, CA USA. [Hanson, Bradley D.] Univ Calif Davis, Davis, CA 95616 USA. [Gerik, James S.] USDA ARS, Parlier, CA USA. EM jbsamtani@ucdavis.edu; bhanson@ucdavis.edu; jgerik@fresno.ars.usda.gov; safennimore@ucdavis.edu; jbweber@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S228 EP S228 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800536 ER PT J AU Scherm, H Krewer, G Cline, WO Harmon, PF Morgan, K Takeda, F AF Scherm, Harald Krewer, Gerard Cline, William O. Harmon, Philip F. Morgan, Kimberly Takeda, Fumiomi TI Advancing Blueberry Production Efficiency by Enabling Mechanical Harvest, Improving Fruit Quality and Safety, and Managing Emerging Diseases SO HORTSCIENCE LA English DT Meeting Abstract C1 [Scherm, Harald] Univ Georgia, Athens, GA 30602 USA. [Krewer, Gerard] Univ Georgia, Tifton, GA USA. [Cline, William O.] N Carolina State Univ, Castle Hayne, NC USA. [Harmon, Philip F.] Univ Florida, Gainesville, FL USA. [Morgan, Kimberly] Mississippi State Univ, Mississippi State, MS 39762 USA. [Takeda, Fumiomi] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM scherm@uga.edu; gkrewer@uga.edu; bill_cline@ncsu.edu; pfharmon@ufl.edu; Morgan@agecon.msstate.edu; Fumi.Takeda@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S199 EP S200 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800479 ER PT J AU Schnell, RJ Kuhn, DN Tondo, CL Motamayor, JC AF Schnell, Raymond J. Kuhn, D. N. Tondo, C. L. Motamayor, J. C. TI The Value of Wild Theobroma Germplasm in Genomics SO HORTSCIENCE LA English DT Meeting Abstract C1 [Schnell, Raymond J.; Kuhn, D. N.; Tondo, C. L.] ARS, USDA, Miami, FL USA. [Motamayor, J. C.] MARS Inc, Miami, FL USA. EM miars@usda-grin.gov; David.Kuhn@ars.usda.gov; Cecile.Tondo@ars.usda.gov; juan.motamayor@effem.com NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S26 EP S26 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800063 ER PT J AU Schreiner, RP Pinkerton, J Zasada, I Bryla, D AF Schreiner, R. Paul Pinkerton, John Zasada, Inga Bryla, David TI The Impact of Ring Nematode (Mesocriconema xenoplax) on Grapevines Grafted onto Different Rootstocks in Field Microplots SO HORTSCIENCE LA English DT Meeting Abstract C1 [Schreiner, R. Paul; Pinkerton, John; Zasada, Inga; Bryla, David] ARS, USDA, HCRL, Corvallis, OR USA. EM paul.schreiner@ars.usda.gov; jack.pinkerton@ars.usda.gov; inga.zasada@ars.usda.gov; david.bryla@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S140 EP S140 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800335 ER PT J AU Simon, PW Roberts, PA AF Simon, Philipp W. Roberts, Philip A. TI Deployment of Nutrient-rich Nematode Resistant Carrots to Benefit Growers, Consumers, and the Environment SO HORTSCIENCE LA English DT Meeting Abstract C1 [Simon, Philipp W.] USDA, Madison, WI USA. [Roberts, Philip A.] Univ Calif Riverside, Riverside, CA 92521 USA. EM psimon@wisc.edu; philip.roberts@ucr.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S283 EP S283 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800664 ER PT J AU Simon, PW Roberts, PA AF Simon, Philipp W. Roberts, Philip A. TI Deployment of Nutrient-rich Nematode Resistant Carrots to Benefit Growers, Consumers, and the Environment (SCRI) SO HORTSCIENCE LA English DT Meeting Abstract C1 [Simon, Philipp W.] USDA, Madison, WI USA. [Roberts, Philip A.] Univ Calif Riverside, Riverside, CA 92521 USA. EM psimon@wisc.edu; philip.roberts@ucr.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S207 EP S208 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800492 ER PT J AU Simon, PW Spooner, D AF Simon, Philipp W. Spooner, David TI New Carrot and Garlic Germplasm to Advance Breeding and Understand Crop Origins SO HORTSCIENCE LA English DT Meeting Abstract C1 [Simon, Philipp W.; Spooner, David] USDA, Madison, WI USA. EM psimon@wisc.edu; david.spooner@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S26 EP S26 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800062 ER PT J AU Slovin, J Rabinowicz, P AF Slovin, Janet Rabinowicz, Pablo TI Abiotic Stress Gene Families in the Diploid Strawberry, Fragaria Vesca SO HORTSCIENCE LA English DT Meeting Abstract C1 [Slovin, Janet] ARS, USDA, Fruit Lab, Beltsville, MD USA. [Rabinowicz, Pablo] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. EM janet.slovin@ars.usda.gov; prabinowicz@som.umaryland.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S18 EP S18 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800040 ER PT J AU Stier, G Kumar, P Jiang, CZ Reid, MS AF Stier, Genevieve Kumar, Pushpendra Jiang, Cai-Zhong Reid, Michael S. TI Silencing of a Proteasome Component Delays Floral Senescence SO HORTSCIENCE LA English DT Meeting Abstract C1 [Stier, Genevieve; Kumar, Pushpendra; Reid, Michael S.] Univ Calif Davis, Davis, CA 95616 USA. [Jiang, Cai-Zhong] ARS, USDA, Davis, CA USA. EM gnstier@ucdavis.edu; drpkdhaka@gmail.com; cjiang@ucdavis.edu; msreid@ucdavis.edu NR 0 TC 3 Z9 3 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S147 EP S147 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800352 ER PT J AU Stover, E Stange, R McCollum, G Jaynes, J AF Stover, Ed Stange, Richard McCollum, Greg Jaynes, Jesse TI Screening Antimicrobial Peptides In Vitro for Use in Developing Huanglongbing and Citrus Canker Resistant Transgenic Citrus SO HORTSCIENCE LA English DT Meeting Abstract C1 [Stover, Ed; Stange, Richard; McCollum, Greg] ARS, USDA, USHRL, Ft Pierce, FL USA. [Jaynes, Jesse] Tuskegee Univ, Tuskegee, AL 36088 USA. EM ed.stover@ars.usda.gov; ric.stange@ars.usda.gov; greg.mccollum@ars.usda.gov; jjaynes@tuskegee.edu NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S120 EP S120 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800289 ER PT J AU Takeda, F AF Takeda, Fumiomi TI Methods for Altering the Flowering Time in Strawberries SO HORTSCIENCE LA English DT Meeting Abstract C1 [Takeda, Fumiomi] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM Fumi.Takeda@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S230 EP S230 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800541 ER PT J AU Tarara, JM Lee, J AF Tarara, Julie M. Lee, Jungmin TI Light and Temperature Effects on Phenolics in Dark-skinned Grapes SO HORTSCIENCE LA English DT Meeting Abstract C1 [Tarara, Julie M.] ARS, USDA, Prosser, WA USA. [Lee, Jungmin] ARS, USDA, HCRL, Parma, ID USA. EM jtarara@wsu.edu; Jungmin.Lee@ars.usda.gov RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S48 EP S48 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800127 ER PT J AU Valenzuela-Estrada, L Vargas, OL Bryla, DR Sullivan, DM Strik, BC AF Valenzuela-Estrada, Luis Vargas, Oscar L. Bryla, David R. Sullivan, Dan M. Strik, Bernadine C. TI Blueberry Root Dynamics and Nutrient Management under Organic and Conventional Production SO HORTSCIENCE LA English DT Meeting Abstract C1 [Valenzuela-Estrada, Luis; Vargas, Oscar L.; Sullivan, Dan M.; Strik, Bernadine C.] Oregon State Univ, Corvallis, OR 97331 USA. [Bryla, David R.] ARS, USDA, Corvallis, OR USA. EM valenzul@onid.orst.edu; vargasoo@hort.oregonstate.edu; brylad@onid.orst.edu; dan.sullivan@oregonstate.edu; strikb@hort.oregonstate.edu NR 0 TC 0 Z9 0 U1 1 U2 17 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S34 EP S34 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800083 ER PT J AU Volk, G Henk, A Richards, C Baldo, A Forsline, PL AF Volk, Gayle Henk, Adam Richards, Christopher Baldo, Angela Forsline, Philip L. TI Diversity in Wild Apple Species of Chinese Origin SO HORTSCIENCE LA English DT Meeting Abstract C1 [Volk, Gayle; Henk, Adam; Richards, Christopher; Forsline, Philip L.] ARS, USDA, Ft Collins, CO USA. EM gvolk@lamar.colostate.edu; adam.henk@ars.usda.gov; Chris.Richards@ars.usda.gov; angela.baldo@ars.usda.gov; plf1@cornell.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S57 EP S57 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800144 ER PT J AU Walker, S Funk, PA AF Walker, Stephanie Funk, Paul A. TI Mechanical Harvest Trials of New Mexican-type Green Chile (Capsicum annuum L.) SO HORTSCIENCE LA English DT Meeting Abstract C1 [Walker, Stephanie] New Mexico State Univ, Las Cruces, NM 88003 USA. [Funk, Paul A.] ARS, USDA, Mesilla Pk, NM USA. EM swalker@nmsu.edu; pfunk@nmsu.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S145 EP S146 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800348 ER PT J AU Walters, T Particka, M Zasada, I Pinkerton, JN AF Walters, Thomas Particka, Michael Zasada, Inga Pinkerton, John N. TI On-farm Trials of Alternatives to Methyl Bromide for Raspberry Nurseries SO HORTSCIENCE LA English DT Meeting Abstract C1 [Walters, Thomas; Particka, Michael] Washington State Univ, Mt Vernon, WA USA. [Zasada, Inga] ARS, USDA, HCRL, Corvallis, OR USA. EM twwalters@wsu.edu; mparticka@wsu.edu; inga.zasada@ars.usda.gov; pinkertj@science.oregonstate.edu RI Walters, Thomas/G-1348-2010 OI Walters, Thomas/0000-0002-2938-8542 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S141 EP S141 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800339 ER PT J AU Wang, H Stier, G Jiang, CZ Reid, MS AF Wang, Hong Stier, Genevieve Jiang, Cai-Zhong Reid, Michael S. TI Controlling Flower Senescence by Inducing Expression of etr1-1, a Mutant Ethylene Receptor SO HORTSCIENCE LA English DT Meeting Abstract C1 [Wang, Hong; Stier, Genevieve; Reid, Michael S.] Univ Calif Davis, Davis, CA 95616 USA. [Jiang, Cai-Zhong] ARS, USDA, Davis, CA USA. EM howang@ucdavis.edu; gnstier@ucdavis.edu; cjiang@ucdavis.edu; msreid@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S160 EP S161 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800384 ER PT J AU Wang, SY Chen, CT Yin, JJ AF Wang, Shiow Ying Chen, Chi-Tsun Yin, Jun-Jie TI Allyl Isothiocyanate Reduces Fruit Decay of Blueberries by Paradoxically Generating Reactive Oxygen Species SO HORTSCIENCE LA English DT Meeting Abstract C1 [Wang, Shiow Ying; Chen, Chi-Tsun] ARS, USDA, Beltsville, MD USA. [Yin, Jun-Jie] US FDA, College Pk, MD USA. EM shiow.wang@ars.usda.gov; wangcysy@gmail.com; junjie.yin@fda.hhs.gov RI Yin, Jun Jie /E-5619-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S214 EP S214 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800505 ER PT J AU Weebadde, CK Crisosto, CH Fazio, G Gallardo, K Main, D Peace, C Reighard, GL Sebolt, A Stone, A van de Weg, E Voorrips, R Xu, KN Lezzoni, A AF Weebadde, Cholani Kumari Crisosto, Carlos H. Fazio, Gennaro Gallardo, Karina Main, Doreen Peace, Cameron Reighard, Gregory L. Sebolt, Audrey Stone, Alexandra van de Weg, Eric Voorrips, Roeland Xu, Kenong Lezzoni, Amy TI Use of PediMap in RosBREED for Visualizing Genetic and Phenotypic Data across Pedigrees in Rosaceous Crops SO HORTSCIENCE LA English DT Meeting Abstract C1 [Weebadde, Cholani Kumari; Sebolt, Audrey; Lezzoni, Amy] Michigan State Univ, E Lansing, MI 48824 USA. [Crisosto, Carlos H.] Univ Calif, Davis, CA USA. [Fazio, Gennaro] ARS, USDA, Geneva, NY USA. [Gallardo, Karina] Washington State Univ, TFREC, Wenatchee, WA USA. [Main, Doreen; Peace, Cameron] Washington State Univ, Pullman, WA 99164 USA. [Sebolt, Audrey] Clemson Univ, Clemson, SC USA. [Stone, Alexandra] Oregon State Univ, Corvallis, OR 97331 USA. [van de Weg, Eric; Voorrips, Roeland] Plant Res Int, Wageningen, Netherlands. [Xu, Kenong] Cornell Univ, Geneva, NY USA. EM weebaddc@msu.edu; carlos@uckac.edu; gf35@cornell.edu; karina_gallardo@wsu.edu; dorrie@wsu.edu; cpeace@wsu.edu; grghrd@clemson.edu; grantzau@msu.edu; stonea@hort.oregonstate.edu; ericvandeweg@wur.nl; roeland.voorrips@wur.nl; kx27@cornell.edu; iezzoni@msu.edu RI Van de Weg, Eric/G-3225-2011; Voorrips, Roeland/H-3458-2011 OI Van de Weg, Eric/0000-0002-9443-5974; Voorrips, Roeland/0000-0003-4388-9244 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S55 EP S55 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800141 ER PT J AU Whitaker, V Plotto, A Baldwin, E Chandler, CK AF Whitaker, Vance Plotto, Anne Baldwin, Elizabeth Chandler, Craig K. TI Progress in Strawberry Breeding at the Univ of Florida: A Historical Variety Trial SO HORTSCIENCE LA English DT Meeting Abstract C1 [Whitaker, Vance; Chandler, Craig K.] Univ Florida IFAS, Wimauma, FL USA. [Plotto, Anne; Baldwin, Elizabeth] ARS, USDA, Winter Haven, FL USA. EM vwhitaker@ufl.edu; anne.plotto@ars.usda.gov; liz.baldwin@ars.usda.gov; ckc@ufl.edu NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S121 EP S121 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800293 ER PT J AU Yang, NN Reighard, GL Ritchie, DF Okie, WR Gasic, K AF Yang, Nannan Reighard, Gregory L. Ritchie, David F. Okie, W. R. Gasic, Ksenija TI Construction of a Genetic Linkage Map for Identification of Molecular Markers Associated with Resistance to Xanthomonas arboriciola pv. pruni in Peach [Prunus persica (L.) Batsch] SO HORTSCIENCE LA English DT Meeting Abstract C1 [Yang, Nannan; Reighard, Gregory L.; Gasic, Ksenija] Clemson Univ, Clemson, SC USA. [Ritchie, David F.] N Carolina State Univ, Raleigh, NC 27695 USA. [Okie, W. R.] ARS, USDA, Byron, GA USA. EM nannany@clemson.edu; grghrd@clemson.edu; david_ritchie@ncsu.edu; william.okie@ars.usda.gov; kgasic@clemson.edu NR 0 TC 1 Z9 1 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S304 EP S305 PG 2 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800718 ER PT J AU Zhang, YL Niu, LX Zhang, QX Jiang, CZ AF Zhang, Yanlong Niu, Lixin Zhang, Qi-Xiang Jiang, Cai-Zhong TI Survey of Wild Lilium Species in the Vicinity of the Qin-Ba Mountains, China SO HORTSCIENCE LA English DT Meeting Abstract C1 [Zhang, Yanlong; Niu, Lixin] NW A&F Univ, Yangling, Shaanxi, Peoples R China. [Zhang, Qi-Xiang] Beijing Forestry Univ, Beijing, Peoples R China. [Jiang, Cai-Zhong] ARS, USDA, Davis, CA USA. EM susan64sg@yahoo.com; niulixinqh@yahoo.com.cn; ninishun@gmail.com; cjiang@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S159 EP S159 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800381 ER PT J AU Zhu, HP Jeon, HY Chen, Y Ozkan, E Derksen, RD Reding, ME Ranger, CM Canas, L Krause, CR Locke, JC Ernst, SC Zondag, RH Fulcher, A Rosetta, R AF Zhu, Heping Jeon, Hong Young Chen, Yu Ozkan, Erdal Derksen, Richard D. Reding, Michael E. Ranger, Christopher M. Canas, Luis Krause, Charles R. Locke, James C. Ernst, Stanley C. Zondag, Randall H. Fulcher, Amy Rosetta, Robin TI Development of Two Intelligent Spray Systems for Ornamental Nursery and Fruit Tree Crops SO HORTSCIENCE LA English DT Meeting Abstract C1 [Zhu, Heping; Jeon, Hong Young; Derksen, Richard D.; Reding, Michael E.; Ranger, Christopher M.; Krause, Charles R.] ARS, USDA, Wooster, OH USA. [Chen, Yu; Ozkan, Erdal; Ernst, Stanley C.] Ohio State Univ, Columbus, OH 43210 USA. [Canas, Luis] Ohio State Univ, Wooster, OH USA. [Locke, James C.] ARS, USDA, Toledo, OH USA. [Zondag, Randall H.] Ohio State Univ Hort & Crop Sci, Columbus, OH USA. [Fulcher, Amy] Univ Kentucky, Lexington, KY USA. [Rosetta, Robin] Oregon State Univ, Aurora, OR USA. EM heping.zhu@ars.usda.gov; hongyoung.jeon@ars.usda.gov; chen.1257@osu.edu; ozkan.2@osu.edu; rich.derksen@ars.usda.gov; mike.reding@ars.usda.gov; christopher.ranger@ars.usda.gov; canas.4@osu.edu; charles.krause@ars.usda.gov; jim.locke@ars.usda.gov; Ernst.1@osu.edu; zondag.1@osu.edu; afulcher@uky.edu; Robin.Rosetta@oregonstate.edu RI Canas, Luis/A-5651-2012; Ernst, Stanley/J-9388-2012 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD AUG PY 2010 VL 45 IS 8 SU S BP S202 EP S202 PG 1 WC Horticulture SC Agriculture GA 822OZ UT WOS:000295060800483 ER PT J AU Webber, CL Shrefler, JW Taylor, MJ AF Webber, Charles L., III Shrefler, James W. Taylor, Merritt J. TI Influence of Corn Gluten Meal on Squash Plant Survival and Yields SO HORTTECHNOLOGY LA English DT Article DE Cucurbita pepo; organic; formulation; direct-seeded; application systems ID SOIL ORGANIC-MATTER; TILLAGE; NITROGEN AB Corn gluten meal (CGM) is a non-selective preemergence or preplant-incorporated herbicide that inhibits root development, decreases shoot length, and reduces plant survival. The development of a mechanized application system for the banded placement of CGM between crop rows (seed row not treated) has increased its potential use in organic vegetable production, especially in direct-seeded vegetables. The objective of this research was to determine the impact of CGM applications (formulations, rates, incorporation, and banded applications) on direct-seeded squash (Cucurbita pepo) plant survival and yields. Neither CGM formulation (powdered or granulated) nor incorporation method (incorporated or non-incorporated) resulted in significant differences in plant survival or squash yields. When averaged across all other factors (formulations, incorporation method, and banding), CGM rates of 250 to 750 g.m(-2) reduced squash survival from 70% to 44%, and squash yields from 6402 to 4472 kg.ha(-1). However, the banded application (CGM placed between rows) resulted in significantly greater crop safety (75% survival) and yield (6402 kg.ha(-1)) than the broadcast (non-banded) applications (35% survival and 4119 kg.ha(-1) yield). It was demonstrated that banded applications of CGM can be useful in direct-seeded squash production and other organic direct-seeded vegetables. C1 [Webber, Charles L., III] USDA ARS, SCARL, Lane, OK 74555 USA. [Shrefler, James W.; Taylor, Merritt J.] Oklahoma State Univ, Wes Watkins Agr Res & Extens Ctr, Lane, OK 74555 USA. RP Webber, CL (reprint author), USDA ARS, SCARL, POB 159, Lane, OK 74555 USA. EM cwebber-usda@lane-ag.org NR 20 TC 3 Z9 3 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2010 VL 20 IS 4 BP 696 EP 699 PG 4 WC Horticulture SC Agriculture GA 642YV UT WOS:000281258800006 ER PT J AU Baldos, OC DeFrank, J Sakamoto, G AF Baldos, Orville C. DeFrank, Joseph Sakamoto, Glenn TI Tolerance of Transplanted Seashore Dropseed to Pre- and Postemergence Herbicides SO HORTTECHNOLOGY LA English DT Article DE Sporobolus virginicus; oxadiazon; oxyfluorfen; sulfosulfuron; native species establishment; roadside re-vegetation; Hawaii ID ZOYSIAGRASS ZOYSIA-JAPONICA; VIRGINICUS L KUNTH; PREEMERGENCE HERBICIDES; PASPALUM TURF; WEED-CONTROL; ESTABLISHMENT; RESTORATION; GRASS AB Field experiments were conducted to assess the tolerance of seashore dropseed (Sporobolus virginicus) to pre- and postemergence herbicides labeled for roadside right-of-way use. Dithiopyr (0.25 and 0.50 lb/acre a.i.), trifluralin + isoxaben (2.0 + 0.5 and 4.0 + 1.0 lb/acre a.i.), oxyfluorfen (0.25 and 0.50 lb/acre a.i.), oxadiazon (2.0 and 4.0 lb/acre a.i.), and granular table salt (99% sodium chloride, 1% sodium silicoaluminate; 83% of particles 0.5-0.25 mm in diameter, 400 lb/acre a.i.) were applied at 2 and 84 days after transplanting (DAT). Pre-emergence weed control with crop response measures as visual foliar injury ratings and aboveground biomass accumulation were recorded 38 days after the second application of herbicides (DAH2). Crop response to postemergence herbicides aminopyralid (1.10 lb/acre a.i.), triclopyr (3.0 lb/acre a.i.), a prepackaged mix of carfentrazone + (4-chloro-2-methylphenoxy)acetic acid + mecoprop + dicamba (0.02 + 1.11 + 0.22 + 0.11 lb/acre a.i.), and sulfosulfuron (0.06 lb/acre a.i.) applied at 70 and 98 DAT included visual foliar injury ratings and aboveground biomass accumulation at 28 DAH2. Although all pre-emergence herbicides (except table salt) exhibited acceptable weed control ratings, only oxadiazon and oxyfluorfen showed exceptional weed control and safety. The postemergence herbicide sulfosulfuron was the least injurious to seashore dropseed. The mixture of carfentrazone + (4-chloro-2-methylphenoxy)acetic acid + mecoprop + dicamba and triclopyr were the most injurious to seashore dropseed and should only be used as a directed spray treatment. An unintended overapplication of aminopyralid was phytotoxic, but it did not lead to complete plant death at 28 DAH2. These data identified oxadiazon, oxyfluorfen, and sulfosulfuron as safe and effective for establishing transplanted seashore dropseed plugs. C1 [Baldos, Orville C.; DeFrank, Joseph] Univ Hawaii Manoa, Dept Trop Plant & Soil Sci, St John Plant Sci Lab 102, Honolulu, HI 96822 USA. [Sakamoto, Glenn] Nat Resources Conservat Serv, USDA, Hoolehua Plant Mat Ctr, Hoolehua, HI 96729 USA. RP Baldos, OC (reprint author), Univ Hawaii Manoa, Dept Trop Plant & Soil Sci, St John Plant Sci Lab 102, 3190 Maile Way, Honolulu, HI 96822 USA. EM obaldos@hawaii.edu NR 37 TC 3 Z9 3 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2010 VL 20 IS 4 BP 772 EP 777 PG 6 WC Horticulture SC Agriculture GA 642YV UT WOS:000281258800019 ER PT J AU Frantz, JM Hand, B Buckingham, L Ghose, S AF Frantz, Jonathan M. Hand, Bryon Buckingham, Lee Ghose, Somik TI Virtual Grower: Software to Calculate Heating Costs of Greenhouse Production in the United States SO HORTTECHNOLOGY LA English DT Article DE decision support; energy efficiency; floriculture; glazing; covering ID TEMPERATURE AB Greenhouses are used in many climates for season extension or year-round production and can be expensive to heat. Greenhouse users and growers are often faced with management decisions that rely on an understanding of how temperature settings, heating systems, fuel types, and construction decisions influence overall heating costs. There are no easy-to-use programs to calculate heating costs associated with these factors over full cropping seasons. A computer program called Virtual Grower was created that helps calculate heating costs at many U.S. sites. The program uses a weather database of typical hourly temperature, light, and wind information of 230 sites from the National Renewable Energy Laboratory in the calculations. A user can define unique design characteristics such as building material and construction style. The user also defines the type of heating system and heating schedule, and then the program will predict heating costs based on typical weather at the selected location. Shorter-term predictions with weather forecasts of 2 days or less can be made with the software if there is an internet connection through integration with local weather forecasts. Virtual Grower can serve as a platform from which many other features can be added, such as plant growth and scheduling. Continued development will improve the software and allow users to perform baseline analysis of their heating costs, identify areas in their production to improve efficiency, and take some of the guesswork out of energy analysis in unique greenhouses. C1 [Frantz, Jonathan M.; Hand, Bryon] ARS, USDA, Toledo, OH 43606 USA. [Buckingham, Lee] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Ghose, Somik] Ctr Innovat Food Technol, Toledo, OH 43615 USA. RP Frantz, JM (reprint author), ARS, USDA, 2801 W Bancroft,Mail Stop 604, Toledo, OH 43606 USA. EM jonathan.frantz@ars.usda.gov NR 17 TC 7 Z9 7 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2010 VL 20 IS 4 BP 778 EP 785 PG 8 WC Horticulture SC Agriculture GA 642YV UT WOS:000281258800020 ER PT J AU Uchendu, EE Muminova, M Gupta, S Reed, BM AF Uchendu, Esther E. Muminova, Magfrat Gupta, Sandhya Reed, Barbara M. TI Antioxidant and anti-stress compounds improve regrowth of cryopreserved Rubus shoot tips SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Blackberry; Lipoic acid; Germplasm; Glutathione; Glycine betaine; Long-term storage; Oxidative stress; Polyvinylpyrrolidone ID ALPHA-LIPOIC ACID; IN-VITRO; LIPID-PEROXIDATION; COLD-ACCLIMATION; ENCAPSULATION-DEHYDRATION; PLANT-REGENERATION; OXIDATIVE STRESS; GLYCINE BETAINE; ASCORBIC-ACID; GLUTATHIONE AB Regrowth of plants after cryopreservation varies, and resulting regrowth ranges from poor to excellent. Oxidative stress is a potential cause of damage in plant tissues. Antioxidants and anti-stress compounds may improve regrowth by preventing or repairing the damage. Lipoic acid (LA), glutathione (GSH), glycine betaine (GB), and polyvinylpyrrolidone (PVP) were tested during cryopreservation of shoot tips using the plant vitrification solution 2 (PVS2) protocol. Two in vitro-grown blackberry cultivars were cold acclimated and then cryopreserved in liquid nitrogen (LN). The antioxidant and anti-stress compounds were added at four critical steps of the protocol: pretreatment, loading, rinsing, and regrowth. Three out of the four compounds significantly improved regrowth of cryopreserved shoot tips. Regrowth ranged from 40% to 50% for controls to > 80% for treated shoot tips. LA (4-8 mM) produced high regrowth at pretreatment, loading, and rinsing for 'Chehalem' and at all steps for 'Hull Thornless'. Recovery improved at all steps with GSH (0.16 mM) and GB (10 mM). PVP had a neutral or negative impact on regrowth. Overall addition of LA, GSH, and GB improved regrowth by similar to 25% over the shoot tips cryopreserved using the regular PVS2 protocol (control). This study shows that adding non-vitamin antioxidants and anti-stress compounds during the PVS2-vitrification protocol improves regrowth of shoot cultures following cryopreservation. We recommend inclusion of antioxidants as part of standard cryopreservation protocols. C1 [Reed, Barbara M.] ARS, USDA, Corvallis, OR 97333 USA. [Uchendu, Esther E.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Muminova, Magfrat] Tashkent Chem Technol Inst, Dept Biotechnol, Tashkent 100011, Uzbekistan. [Gupta, Sandhya] Natl Bur Plant Genet Resources, Tissue Culture & Cryopreservat Unit, New Delhi 110012, India. RP Reed, BM (reprint author), ARS, USDA, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM Barbara.Reed@ARS.USDA.GOV OI Reed, Barbara/0000-0003-0079-8473 FU USDA-ARS [5358-21000-03300D] FX This study was funded by CRIS project 5358-21000-03300D USDA-ARS. Thanks to Drs. Tony Chen and Ralph Reed for very useful advice and to the Ford Foundation International Fellowship Program and the University Club of Portland Inc. for awards to Esther Uchendu. Dr. Magfrat Muminova acknowledges the International Union of Biochemistry and Molecular Biology (IUBMB) for awarding a Wood-Whelan Fellowship. NR 62 TC 35 Z9 35 U1 1 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2010 VL 46 IS 4 BP 386 EP 393 DI 10.1007/s11627-010-9292-9 PG 8 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 622JD UT WOS:000279657400008 ER PT J AU Yang, YL Zhu, YC Ottea, J Husseneder, C Leonard, BR Abel, C Huang, FN AF Yang, Yunlong Zhu, Yu Cheng Ottea, James Husseneder, Claudia Leonard, B. Rogers Abel, Craig Huang, Fangneng TI Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Aminopeptidase N; Diatraea saccharalis; Cry1Ab resistance; Molecular cloning; Total aminopeptidase N activity; Quantitative RT-PCR; RNAi ID DOUBLE-STRANDED-RNA; HELIOTHIS-VIRESCENS LARVAE; DELTA-ENDOTOXIN BINDING; BRUSH-BORDER MEMBRANE; HELICOVERPA-ARMIGERA; PLUTELLA-XYLOSTELLA; PORE FORMATION; TOXIN-BINDING; CRY1AB TOXIN; BOMBYX-MORI AB Aminopeptidase N (APN) proteins located at the midgut epithelium of some lepidopteran species have been implicated as receptors for insecticidal proteins from Bacillus thuringiensis. cDNAs of three APN isoforms, DsAPN1, DsAPN2, and DsAPN3, from Cry1Ab-susceptible (Cry1Ab-SS) and -resistant (Cry1Ab-RR) strains of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), were identified and sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5' rapid amplification of cDNA end (5' RACE). The characteristic APN sequence features were derived from deduced amino acid sequences of the cloned cDNAs. cDNA sequences of the three APN genes were identical between the Cry1Ab-SS and -RR strains. However, total APN proteolytic activity and gene expression of the three APNs from Cry1Ab-RR larvae were significantly lower than those of the Cry1Ab-SS strain. RNA interference (RNAi) was employed using an oral droplet feeding technique for the three APNs of the Cry1Ab-SS strain. Down-regulating expressions of the three APN genes by RNAi were corresponding to the reductions in the specific APN activity. In addition, silencing of all three APNs in D. saccharalis in vivo by RNAi resulted in a decrease in Cry1Ab susceptibility. Our results showed that reduction in expression of the three APNs is functionally associated with the Cry1Ab resistance in D. saccharalis. Published by Elsevier Ltd. C1 [Zhu, Yu Cheng; Abel, Craig] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. [Yang, Yunlong; Ottea, James; Husseneder, Claudia; Leonard, B. Rogers; Huang, Fangneng] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. RP Zhu, YC (reprint author), USDA ARS, Jamie Whitten Delta States Res Ctr, POB 346,141 Expt Stn Rd, Stoneville, MS 38776 USA. EM yc.zhu@ars.usda.gov FU Board of Regents of the State of Louisiana [LEQSF 2006-09-RD-A-0]; USDA-ARS [58-6402-6-035] FX We thank Drs. Mukti Ghimire, Qiuying Huang, Dee Colby, Amit Sethi for reviewing an earlier draft of the manuscript. The authors also thank Sandy West for insect rearing. This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript number 2010-234-4300. This project represents work supported by the Board of Regents of the State of Louisiana under Contract No. LEQSF 2006-09-RD-A-0 and USDA-ARS under Contract No. 58-6402-6-035. NR 73 TC 40 Z9 44 U1 0 U2 31 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD AUG PY 2010 VL 40 IS 8 BP 592 EP 603 DI 10.1016/j.ibmb.2010.05.006 PG 12 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 634WK UT WOS:000280616400004 PM 20685334 ER PT J AU Khajuria, C Buschman, LL Chen, MS Muthukrishnan, S Zhu, KY AF Khajuria, Chitvan Buschman, Lawrent L. Chen, Ming-Shun Muthukrishnan, Subbaratnam Zhu, Kun Yan TI A gut-specific chitinase gene essential for regulation of chitin content of peritrophic matrix and growth of Ostrinia nubilalis larvae SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Chitinase; Chitin synthase; European corn borer; Ostrinia nubilalis; Peritrophic matrix; RNA interference ID SITE-DIRECTED MUTAGENESIS; FUNCTIONAL EXPRESSION; ANOPHELES-GAMBIAE; MOLECULAR-CLONING; INSECT CHITINASE; MANDUCA-SEXTA; AEDES-AEGYPTI; CDNA; MIDGUT; PERMEABILITY AB Chitinases belong to a large and diverse family of hydrolytic enzymes that break down glycosidic bonds of chitin. However, very little is known about the function of chitinase genes in regulating the chitin content in peritrophic matrix (PM) of the midgut in insects. We identified a cDNA putatively encoding a chitinase (OnCht) in European corn borer (ECB; Ostrinia nubilalis). The OnCht transcript was predominately found in larval midgut but undetectable in eggs, pupae, or adults. When the larvae were fed on an artificial diet, the OnCht transcript level increased by 4.4-fold but the transcript level of a gut-specific chitin synthase (OnCHS2) gene decreased by 2.5-fold as compared with those of unfed larvae. In contrast, when the larvae were fed with the food and then starved for 24 h, the OnCht transcript level decreased by 1.8-fold but the transcript level of OnCHS2 increased by 1.8-fold. Furthermore, there was a negative relationship between OnCht transcript level and chitin content in the midgut. By using a feeding-based RNAi technique, we were able to reduce the OnCht transcript level by 63-64% in the larval midgut Consequently, these larvae showed significantly increased chitin content (26%) in the PM but decreased larval body weight (54%) as compared with the control larvae fed on the diet containing GFP dsRNA. Therefore, for the first time, we provide strong evidence that OnCht plays an important role in regulating chitin content of the PM and subsequently affecting the growth and development of the ECB larvae. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Khajuria, Chitvan; Buschman, Lawrent L.; Chen, Ming-Shun; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Chen, Ming-Shun] USDA ARS, Plant Sci & Entomol Res Unit, Manhattan, KS 66506 USA. [Muthukrishnan, Subbaratnam] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. RP Zhu, KY (reprint author), Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. EM kzhu@ksu.edu RI Khajuria, Chitvan/E-7690-2012 FU Kansas Agricultural Experiment Station; Arthropod Genomics Center; Kansas State University FX This study was supported in part by the Kansas Agricultural Experiment Station and the Arthropod Genomics Center funded by K-State Targeted Excellence program at Kansas State University. This paper is contribution No 10-146-J from the Kansas Agricultural Experiment Station. The Ostrinia nubilalis voucher specimens (voucher No. 079) are located in the Kansas State University Museum of Entomological and Prairie Arthropod Research, Manhattan, Kansas. NR 47 TC 40 Z9 53 U1 0 U2 24 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD AUG PY 2010 VL 40 IS 8 BP 621 EP 629 DI 10.1016/j.ibmb.2010.06.003 PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 634WK UT WOS:000280616400007 PM 20542114 ER PT J AU Ulyshen, MD Soon, V Hanula, JL AF Ulyshen, Michael D. Soon, Villu Hanula, James L. TI On the vertical distribution of bees in a temperate deciduous forest SO INSECT CONSERVATION AND DIVERSITY LA English DT Article DE Arboreal; arthropods; biodiversity; bottomland hardwood forests; floodplains; insects; pollinators; sweat bees; vertical stratification; wetlands; window traps ID COMMUNITY COMPOSITION; RAIN-FOREST; PAN TRAPS; DIVERSITY; CANOPY; UNDERSTORY; STRATA; STRATIFICATION; PREFERENCES; HYMENOPTERA AB 1. Despite a growing interest in forest canopy biology, very few studies have examined the vertical distribution of forest bees. In this study, bees were sampled using 12 pairs of flight-intercept traps suspended in the canopy (>= 15 m) and near the ground (0.5 m) in a bottomland hardwood forest in the southeastern United States. 2. In total, 6653 bees from 5 families and 71 species were captured. Augochlora pura (Say) (Halictidae), accounted for over 91% of all bees collected and was over 40 times more abundant on average in the canopy than near the ground. 3. Even after removing A. pura from the dataset, bee abundance, richness and Shannon's diversity were considerably higher in the canopy than near the ground. 4. According to both non-metric multidimensional scaling and analysis of similarities, the bee community in the canopy was distinct from that near the ground. Based on indicator species analysis, A. pura, Lasioglossum imitatum (Smith), Lasioglossum versatum sensu Mitch., and Lasioglossum zephyrum (Smith) were significantly associated with the canopy whereas Andrena personata Robertson and Lasioglossum macoupinense (Robertson) were significantly associated with the ground. 5. Augochlora pura was consistently more abundant in the canopy than near the ground throughout the season, but was more so in mid-to-late summer (i.e., June-September), a period coinciding with low floral resource availability. As a group, the remaining bee community exhibited a similar pattern. 6. We suspect that bees frequent the canopy, particularly during times of low nectar and pollen availability, to acquire non-floral resources such as honeydew and sap. C1 [Ulyshen, Michael D.; Hanula, James L.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. [Soon, Villu] Univ Tartu, Dept Zool, Inst Ecol & Earth Sci, EE-50090 Tartu, Estonia. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM mulyshen@hotmail.com NR 40 TC 20 Z9 20 U1 3 U2 35 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1752-458X J9 INSECT CONSERV DIVER JI Insect. Conserv. Divers. PD AUG PY 2010 VL 3 IS 3 BP 222 EP 228 DI 10.1111/j.1752-4598.2010.00092.x PG 7 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA 623KU UT WOS:000279739400007 ER PT J AU Roehrdanz, R Heilmann, L Senechal, P Sears, S Evenson, P AF Roehrdanz, R. Heilmann, L. Senechal, P. Sears, S. Evenson, P. TI Histone and ribosomal RNA repetitive gene clusters of the boll weevil are linked in a tandem array SO INSECT MOLECULAR BIOLOGY LA English DT Article DE histones; ribosomal RNA; Anthonomus; repetitive sequences; internal transcribed spacers ID MELANOGASTER SPECIES SUBGROUP; DROSOPHILA-MELANOGASTER; CHROMOSOMAL LOCATION; NUCLEOTIDE-SEQUENCE; MITOCHONDRIAL-DNA; MULTIGENE FAMILY; REPEATING UNITS; RDNA GENES; EVOLUTION; COLEOPTERA AB Histones are the major protein component of chromatin structure. The histone family is made up of a quintet of proteins, four core histones (H2A, H2B, H3 & H4) and the linker histones (H1). Spacers are found between the coding regions. Among insects this quintet of genes is usually clustered and the clusters are tandemly repeated. Ribosomal DNA contains a cluster of the rRNA sequences 18S, 5.8S and 28S. The rRNA genes are separated by the spacers ITS1, ITS2 and IGS. This cluster is also tandemly repeated. We found that the ribosomal RNA repeat unit of at least two species of Anthonomine weevils, Anthonomus grandis and Anthonomus texanus (Coleoptera: Curculionidae), is interspersed with a block containing the histone gene quintet. The histone genes are situated between the rRNA 18S and 28S genes in what is known as the intergenic spacer region (IGS). The complete reiterated Anthonomus grandis histone-ribosomal sequence is 16 248 bp. C1 [Roehrdanz, R.; Heilmann, L.; Sears, S.; Evenson, P.] ARS, Biosci Res Lab, Red River Valley Agr Res Ctr, USDA, Fargo, ND 58105 USA. [Senechal, P.] N Dakota State Univ, Dept Biochem, Fargo, ND 58105 USA. RP Roehrdanz, R (reprint author), ARS, Biosci Res Lab, Red River Valley Agr Res Ctr, USDA, POB 5674, Fargo, ND 58105 USA. EM richard.roehrdanz@ars.usda.gov NR 38 TC 13 Z9 14 U1 0 U2 6 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1075 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2010 VL 19 IS 4 BP 463 EP 471 DI 10.1111/j.1365-2583.2010.01006.x PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 619RE UT WOS:000279446800005 PM 20456508 ER PT J AU Pasiakos, SM McClung, HL McClung, JP Urso, ML Pikosky, MA Cloutier, GJ Fielding, RA Young, AJ AF Pasiakos, Stefan M. McClung, Holly L. McClung, James P. Urso, Maria L. Pikosky, Matthew A. Cloutier, Gregory J. Fielding, Roger A. Young, Andrew J. TI Molecular Responses to Moderate Endurance Exercise in Skeletal Muscle SO INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM LA English DT Article DE protein synthesis; protein breakdown; muscle protein turnover; translation initiation; intracellular signaling ID GLYCOGEN-SYNTHASE KINASE-3; PROTEIN-SYNTHESIS; RESISTANCE EXERCISE; TIME-COURSE; PATHWAY; PHOSPHORYLATION; EXPRESSION; RECOVERY; INSULIN; METABOLISM AB This study examined alterations in skeletal-muscle growth and atrophy-related molecular events after a single bout of moderate-intensity endurance exercise. Muscle biopsies were obtained from 10 men (23 +/- 1 yr, body mass 80 +/- 2 kg, and VO(2peak) 45 +/- 1 ml . kg(-1) . min(-1)) immediately (0 hr) and 3 hr after a 60-min bout of cycle exercise (60% +/- 5% VO(2peak)). Corresponding muscle biopsies were also obtained under resting conditions. The phosphorylation status of insulin/IGF-PI3K molecular-signaling proteins, ubiquitin-proteasome-related gene expression, FOXO transcription factors, and myogenic regulatory factors in muscle samples was analyzed using multiplex analysis, Western blotting, and quantitative real-time polymerase chain reaction (qRT-PCR). A condition-time interaction was observed for Akt phosphorylation (p < .05) with multiplexing. Regardless of endurance exercise, Akt phosphorylation decreased and ERK phosphorylation increased at 3 hr compared with 0 hr (p < .05). Levels of p70(S6K) phosphorylation were 110% greater (p < .05) at 3 hr than at 0 hr using Western blots. MuRF mRNA expression postexercise increased; levels were 4.7- and 5.7-fold greater (p < .05) at 0 hr and 3 hr, respectively, than at rest with qRT-PCR. Atrogin mRNA expression was up-regulated 3.2-fold 3 hr postexercise compared with rest. These findings demonstrate modest changes in the molecular responses to moderate endurance exercise in the absence of nutrition. This study provides the groundwork for future investigations designed to optimize the metabolic conditions necessary to positively influence the cellular mechanisms specific to skeletal-muscle protein turnover during recovery from endurance exercise. C1 [Pasiakos, Stefan M.; McClung, Holly L.; McClung, James P.; Pikosky, Matthew A.; Young, Andrew J.] USA, Environm Med Res Inst, Mil Nutr Div, Natick, MA 01760 USA. [Urso, Maria L.] USA, Environm Med Res Inst, Mil Performance Div, Natick, MA 01760 USA. [Cloutier, Gregory J.; Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Pasiakos, SM (reprint author), USA, Environm Med Res Inst, Mil Nutr Div, Natick, MA 01760 USA. RI McClung, James/A-1989-2009; Pasiakos, Stefan/E-6295-2014 OI Pasiakos, Stefan/0000-0002-5378-5820 FU U S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This material is based on work supported by the U S. Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U. S. Dept of Agriculture. NR 36 TC 19 Z9 20 U1 1 U2 6 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1526-484X J9 INT J SPORT NUTR EXE JI Int. J. Sport Nutr. Exerc. Metab. PD AUG PY 2010 VL 20 IS 4 BP 282 EP 290 PG 9 WC Nutrition & Dietetics; Sport Sciences SC Nutrition & Dietetics; Sport Sciences GA 640BT UT WOS:000281023000002 PM 20739716 ER PT J AU Du, ZJ Jordan, EM Rooney, AP Chen, GJ Austin, B AF Du, Zong-Jun Jordan, Elizabeth M. Rooney, Alejandro P. Chen, Guan-Jun Austin, Brian TI Corynebacterium marinum sp nov isolated from coastal sediment SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; RENATURATION RATES; DNA HYBRIDIZATION; WILD PENGUINS; CHINA; ACID; SOIL AB A taxonomic study was performed on strain D7015(T), which was isolated from coastal sediment close to a coal-fired power station in Qingdao, China. Cells of strain D7015(T) were Gram-positive, non-motile, diphtheroid rods that grew in the presence of 0-8% (w/v) NaCl and at 4-37 degrees C, with optimum growth at 1 % (w/v) NaCl and 30-32 degrees C. The DNA G+C content was 65.0 mol%. The major fatty acids were C(18:1)omega 9c (56.18%), C(16:0) (38.02%), C(16:1)omega 7c (4.45%), C(18:0) (1.0%) and C(14:0) (0.35%). On the basis of morphological, physiological and phylogenetic characteristics, strain D7015(T) was classified in the genus Corynebacterium. It exhibited a 16S rRNA gene sequence similarity of 95.9% and a DNA DNA relatedness value of 20.4% with Corynebacterium halotolerans DSM 44683(T). Strain D7015(T) was sufficiently different from recognized species of the genus Corynebacterium to be considered to represent a novel species. The name Corynebacterium marinum sp. nov. is proposed, with strain D7015(T) (=CGMCC 1.6998(T)=NRRL B-24779(T)) as the type strain. C1 [Jordan, Elizabeth M.; Austin, Brian] Heriot Watt Univ, Sch Life Sci, Edinburgh EH14 4AS, Midlothian, Scotland. [Du, Zong-Jun; Chen, Guan-Jun] Shandong Univ, Coll Marine Sci, Weihai 264209, Peoples R China. [Austin, Brian] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland. [Rooney, Alejandro P.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Austin, B (reprint author), Heriot Watt Univ, Sch Life Sci, Edinburgh EH14 4AS, Midlothian, Scotland. EM brian.austin@stir.ac.uk FU Darwin Initiative Project in the UK [162/8/065]; National Science Foundation of China [40730847]; Ministry of Agriculture of China [07-046]; Key Laboratory of Marine Bioactive Substances [MBSMAT-2009-07]; Modern Analytical Techniques, State Oceanic Administration (SOA), China FX This work was supported by the Darwin Initiative Project in the UK (no. 162/8/065), the National Science Foundation of China (project no. 40730847), a Special Non-profit Research Project from the Ministry of Agriculture of China (nyhyzx 07-046) and a grant (MBSMAT-2009-07) from the Key Laboratory of Marine Bioactive Substances and Modern Analytical Techniques, State Oceanic Administration (SOA), China. NR 25 TC 9 Z9 10 U1 0 U2 3 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD AUG PY 2010 VL 60 BP 1944 EP 1947 DI 10.1099/ijs.0.018523-0 PN 8 PG 4 WC Microbiology SC Microbiology GA 642AS UT WOS:000281175700041 PM 19783605 ER PT J AU Eggleston, G Montes, B Antoine, A Stewart, D AF Eggleston, Gillian Montes, Belisario Antoine, April Stewart, David TI Seasonal variations in optimized applications of intermediate temperature stable alpha-amylase in raw sugar manufacture SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE alpha-amylase; Bacillus subtilis seasonal starch variations; next-to-the-last evaporators; raw sugar manufacture ID STARCH AB In recent years, starch being delivered to and processed in U.S. factories has risen markedly because of the increased production of green (unburnt) and combine-harvested (billeted) sugarcane as well as the introduction of new sugarcane varieties with higher starch content. This has led to warnings by some U.S. refineries that there may be a penalty for high starch concentrations in raw sugar if starch control is not improved. To prevent carry-over alpha-amylase activity in molasses and raw sugar in the U.S., commercial alpha-amylases used to control starch are intermediate temperature (IT) stable and sourced from Bacillus subtilis bacteria. alpha-Amylases have been typically applied to syrup in last evaporators where starch is solubilized and gelatinized, syrup temperatures are similar to 60-65 degrees C, and similar to 18 min retention time (R(t)) is available. As IT stable alpha-amylases are effective up to 85 degrees C, they could be more effective and economical if applied to next-to-the-last evaporators where syrup temperatures are similar to 77 degrees C. Factory alpha-amylase trials were conducted across the 2007 Louisiana processing season (Oct-Dec). Application of a working solution (diluted 3-fold in water at the factory) of IT stable alpha-amylase of high activity per unit cost (118.3 KNU/m1/$) to the next-to-the-last evaporator provided significantly (P<.05) greater starch hydrolysis (up to 78.0% at a 5 ppm/cane wt dose) than applying it to the last evaporator alone (only up to 59.8% at a 5 ppm/cane wt dose). Reasons for the improved starch hydrolysis in the next-to-the-last than the last evaporator are multi-fold: (i) the lower Brix levels in the next-to-the last evaporator improve alpha-amylase action, (ii) more water is available as a reactant for the hydrolysis reaction, and (iii) there is more time for the hydrolysis reaction to occur. Starch hydrolysis, generally, increased polynomially with increasing initial concentrations of starch in syrups. Seasonal variations in starch concentrations affected the application of alpha-amylase to next-to-the-last evaporator more than to the last evaporator alone. Significantly (P<.05) less starch was hydrolyzed with lower precision when starch concentrations were <1000 ppm/Brix in late season (Dec), because of lower contact between the starch and alpha-amylase. Fluctuating starch concentrations across the season make standardized application of alpha-amylase impossible. Final recommendations are provided. C1 [Eggleston, Gillian; Antoine, April] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Montes, Belisario; Stewart, David] Alma Plantat LLC, Lakeland, LA 70752 USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov FU American Sugar Cane League FX The authors thank the American Sugar Cane League for contributing funds to this research and to Mr. Eldwin St. Cyr for excellent technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 16 TC 11 Z9 11 U1 0 U2 1 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD AUG PY 2010 VL 112 IS 1340 BP 472 EP 480 PG 9 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA 640UF UT WOS:000281078100022 ER PT J AU Dudek, EJ Lampi, KJ Lampi, JA Shang, F King, J Wang, YL Taylor, A AF Dudek, Edward J. Lampi, Kirsten J. Lampi, Jason A. Shang, Fu King, Jonathan Wang, Yongling Taylor, Allen TI Ubiquitin Proteasome Pathway-Mediated Degradation of Proteins: Effects Due to Site-Specific Substrate Deamidation SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Article ID EYE LENS TRANSPARENCY; END RULE PATHWAY; X-RAY-ANALYSIS; ALPHA-CRYSTALLIN; GAMMA-CRYSTALLIN; BETA-CRYSTALLIN; POSTTRANSLATIONAL MODIFICATIONS; ENDOPLASMIC-RETICULUM; MOLECULAR CHAPERONE; PROTEOLYTIC PATHWAY AB PURPOSE. The accumulation, aggregation, and precipitation of proteins is etiologic for age-related diseases, particularly cataract, because the precipitates cloud the lens. Deamidation of crystallins is associated with protein precipitation, aging, and cataract. Among the roles of the ubiquitin proteasome pathway (UPP) is protein surveillance and maintenance of protein quality. The purpose of this study was to determine whether deamidation can alter clearance of crystallins by the UPP. METHODS. Wild-type (WT) and deamidated crystallins were expressed and (125)I-radiolabeled. Ubiquitination and degradation were monitored separately. RESULTS. For beta B2 crystallins, rates of ubiquitination and adenosine triphosphate-dependent degradation, both indicators of active UPP, occurred in the order Q70E/Q162E>Q162E> Q70E = WT beta B2 using reticulocyte lysate as the source of degradation machinery. Human lens epithelial cell lysates and lens fiber cell lysates also catalyzed ubiquitination but only limited degradation. Supplementation with proteasome failed to enhance degradation. Rates of ubiquitination and degradation of WT and deamidated beta B1 crystallins were rapid and showed little relationship to the site of deamidation using N157D and Q204E mutants. gamma D-Crystallins were not degraded by the UPP. Deamidation altered amine reactivity, circular dichroism spectra, surface hydrophobicity, and thermal stability. CONCLUSIONS. These data demonstrate for the first time that, like mild oxidative stress, deamidation of some proteins makes them preferred substrates for ubiquitination and, in some cells, for UPP-dependent degradation. Failure to properly execute ubiquitination and degrade the ubiquitin-conjugates may ex-plain their accumulation on aging and in cataractogenesis. (Invest Ophthalmol Vis Sci. 2010;51:4164-4173) DOI:10.1167/iovs.09-4087 C1 [Dudek, Edward J.; Shang, Fu; Taylor, Allen] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. [Lampi, Kirsten J.; Lampi, Jason A.] Oregon Hlth & Sci Univ, Sch Dent, Dept Integrat Biosci, Portland, OR 97201 USA. [King, Jonathan; Wang, Yongling] MIT, Dept Biol, Cambridge, MA USA. RP Taylor, A (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, 711 Washington Ave, Boston, MA 02111 USA. EM allen.taylor@tufts.edu RI Wang, Yongting/C-2916-2014 FU National Institutes of Health [EY13250, EY012239, EY011717]; Johnson Johnson; American Health Assistance Foundation; United States Department of Agriculture [1950-510000-060-01a] FX Supported by National Institutes of Health Grants EY13250 (AT), EY012239 (KL), and EY011717 (FS); Johnson & Johnson Focused Giving Program (AT); American Health Assistance Foundation (AT); and United States Department of Agriculture Grant 1950-510000-060-01a (AT). NR 85 TC 13 Z9 14 U1 2 U2 10 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD AUG PY 2010 VL 51 IS 8 BP 4164 EP 4173 DI 10.1167/iovs.09-4087 PG 10 WC Ophthalmology SC Ophthalmology GA 629JT UT WOS:000280194100047 PM 20592226 ER PT J AU Eichman, H Hunt, GL Kerkvliet, J Plantinga, AJ AF Eichman, Henry Hunt, Gary L. Kerkvliet, Joe Plantinga, Andrew J. TI Local Employment Growth, Migration, and Public Land Policy: Evidence from the Northwest Forest Plan SO JOURNAL OF AGRICULTURAL AND RESOURCE ECONOMICS LA English DT Article DE amenities; employment growth; migration; Northwest Forest Plan; old-growth forests; public land management ID WESTERN UNITED-STATES; COUNTY GROWTH; REDISTRIBUTION; DETERMINANTS; CONVERGENCE; MANAGEMENT; ECONOMIES; AMENITIES; QUALITY; REGIONS AB Debates over protecting public land reveal two views. Some argue protection reduces commodity production, reducing local employment and increasing out-migration. Others contend protection produces amenities that support job growth and attract migrants. We test these competing views for the Northwest Forest Plan (NWFP), which reallocated 11 million acres of federal land from timber production to protecting old-growth forest species. We find evidence that land protection directly reduced local employment growth and increased net migration. The total negative effect on employment was offset only slightly by positive migration-driven effects. Employment losses were concentrated in metropolitan counties, but percentage losses were higher in rural counties. C1 [Eichman, Henry] USFS TEAMS Enterprise, Duluth, MN USA. [Hunt, Gary L.] Univ Maine, Sch Econ, Orono, ME 04469 USA. [Kerkvliet, Joe] Wilderness Soc, Bozeman, MT USA. [Plantinga, Andrew J.] Oregon State Univ, Dept Agr & Resource Econ, Corvallis, OR 97331 USA. RP Eichman, H (reprint author), USFS TEAMS Enterprise, Duluth, MN USA. NR 43 TC 7 Z9 8 U1 3 U2 18 PU WESTERN AGRICULTURAL ECONOMICS ASSOC PI LOGAN PA C/O DEEVON BAILEY, UTAH STATE UNIV, ECONOMICS DEPT, 3535 OLD MAIN HILL, LOGAN, UT 84322-3530 USA SN 1068-5502 J9 J AGR RESOUR ECON JI J. Agric. Resour. Econ. PD AUG PY 2010 VL 35 IS 2 BP 316 EP 333 PG 18 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 639YT UT WOS:000281013800009 ER PT J AU Oliver, WT Miles, JR AF Oliver, W. T. Miles, J. R. TI A low-fat liquid diet increases protein accretion and alters cellular signaling for protein synthesis in 10-day-old pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE body composition; energy source; protein synthesis; swine ID SKELETAL-MUSCLE; ENERGY-INTAKE; NEONATAL PIGS; AMPK; PATHWAY; ACID; RATS; DEPOSITION; INSULIN; KINASE AB Previous research showed that neonatal pigs respond to decreases in energy density of liquid diets with increased feed intake, resulting in similar performance to pigs fed a more energy-dense diet. The objective of this experiment was to determine whether a high- (25%, HF) or low-fat (2%, LF) liquid diet would affect nutrient accretion rate and select proteins involved in energy homeostasis and protein synthesis in early weaned pigs. Ninety-six pigs, with an initial BW of 3,637 +/- 85 g, were weaned from the sow at 10 d of age and utilized in a randomized complete block design. Pigs were blocked by BW and then assigned to pens (8 pigs/pen). Diets were formulated to provide a constant AA: ME ratio and were fed for 10 d. Pigs were killed at 10, 15, and 20 d of age, at which time blood and LM were collected, and carcasses were prepared for body composition analysis. Blood was analyzed for plasma urea nitrogen (PUN) and NEFA. Longissimus dorsi was analyzed via western immunoblot for mammalian target of rapamycin (mTOR) and adenosine 5' monophosphate-activated protein kinase (AMPK) phosphorylation. Pigs gained 347 +/- 11 g/d, which resulted in an ending BW of 6,858 +/- 135 g, regardless of dietary treatment (P > 0.49). Pigs fed the LF diet consumed 25% more milk than pigs fed the HF diet (2,853 +/- 86 vs. 2,269 +/- 79 g dry feed.pen(-1).d(-1); P < 0.01), which resulted in similar calculated ME intakes between dietary treatments (9.9 +/- 0.2 vs. 10.5 +/- 0.2 Mcal.pen(-1).d(-1); P > 0.5). Feed conversion (G: F) was 24% greater in HF-fed compared with LF-fed pigs (P < 0.01). Circulating NEFA (40 +/- 14 vs. 138 +/- 21 mu Eq/L; P < 0.01) and PUN (3.0 +/- 0.6 vs. 17.7 +/- 0.8 mM; P < 0.01) concentrations were less in LF pigs compared with HF pigs after 10 d of dietary treatments. Pigs consuming the LF diet had a 21% increase in protein accretion (50.5 +/- 2.8 vs. 61.2 +/- 2.8 g/d; P < 0.04) and a 71% reduced lipid accretion rate (28.8 +/- 2.0 vs. 8.3 +/- 2.3 g/d; P < 0.001). Phosphorylation of AMPK was 29% less (P < 0.03) in LF pigs compared with HF pigs, whereas mTOR phosphorylation was increased by 37% in LF pigs (P < 0.01). We conclude that feeding a LF liquid diet to pigs weaned from the sow at 10 d of age increases feed intake to regulate energy intake while maintaining growth performance. In addition, 10-d-old pigs consuming a liquid LF diet have increased protein deposition by a mechanism mediated through AMPK and mTOR. C1 [Oliver, W. T.; Miles, J. R.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Oliver, WT (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM William.Oliver@ars.usda.gov NR 33 TC 8 Z9 9 U1 0 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2010 VL 88 IS 8 BP 2576 EP 2584 DI 10.2527/jas.2009-2766 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 624DQ UT WOS:000279796800004 PM 20382868 ER PT J AU Graham, TW Breher, JE Farver, TB Cullor, JS Kehrli, ME Oberbauer, AM AF Graham, T. W. Breher, J. E. Farver, T. B. Cullor, J. S. Kehrli, M. E., Jr. Oberbauer, A. M. TI Biological markers of neonatal calf performance: The relationship of insulin-like growth factor-I, zinc, and copper to poor neonatal growth SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE calf; copper; growth; inflammation; insulin-like growth factor; zinc ID ANGUS BEEF-CATTLE; HEIFER MANAGEMENT DECISIONS; FACTOR BINDING-PROTEINS; HOLSTEIN HEIFERS; BODY-WEIGHT; REPLACEMENT HEIFERS; CARCASS COMPOSITION; FEED-EFFICIENCY; TRANSGENIC MICE; IMMUNE-SYSTEM AB Raising a heifer calf to reproductive age represents an enormous cost to the producer. Poor neonatal growth exacerbates the costs incurred for rearing, and use of blood variables that may be associated with poorly growing calves may offer predictive value for growth and performance. Thus, the principal objective of the present study was to describe changes in serum IGF-I, zinc, and copper from birth to 90 d in Holstein calves, while accounting for sex and twin status, in poorly growing calves and calves growing well. A second objective was to test the hypothesis that an association exists between these serum variables and morphometric indicators of growth. Measurements of BW, length, and height were recorded at birth and at 30, 60, and 90 d of age. Jugular blood (12 mL) was collected from each calf on d 1 to determine serum total protein, serum IgG, packed cell volume, serum zinc, serum copper, serum IGF-I, and CD18 genotype for bovine leukocyte adhesion deficiency; serum zinc, serum copper, and serum IGF-I ( predictor variables) were also determined for each calf on d 2 through 10 and on d 30, 60, and 90. Stepwise multiple regression and logistic regression analyses were used to examine the relationships between the predictor variables and the dependent variables (BW, height, and length at d 30, 60, and 90 of life). Birth weight, sex, serum IGF-I ( at all ages), serum copper, and the serum copper-to-zinc ratio were associated, to varying degrees, with the dependent growth variables. Birth weight was consistently the dominant predictor. In conclusion, these results suggest that lighter birth weight, reduced serum IGF-I, and inflammation may be important causes of poor growth in neonatal Holstein dairy calves. C1 [Graham, T. W.; Breher, J. E.] Vet Consulting Serv, Davis, CA 95618 USA. [Farver, T. B.; Cullor, J. S.] Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA. [Kehrli, M. E., Jr.] ARS, Natl Anim Dis Ctr, USDA, Swine & Prion Dis Res Unit, Ames, IA 50010 USA. [Oberbauer, A. M.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. RP Graham, TW (reprint author), Vet Consulting Serv, Davis, CA 95618 USA. EM tgraham@dcn.davis.ca.us FU Veterinary Consulting Services; Zinpro Corp.; USDA FX This study was supported in part with funds provided by Veterinary Consulting Services (Davis, CA), the Zinpro Corp. (Eden Prairie, MN), and the USDA. The authors thank Michele Chin, Peter Diehl, Nicole Ekblom, Lara Bosh, and Amanda Plunkett (Veterinary Consulting Services) for technical assistance with care and handling of the calves; A. L. Gilbert Company (Oakdale, CA) for assistance in formulation and production of the calf grains used in this trial; Ronald Haile, Gabe Azevedo, and Tony Silveira of Foster Farms (Modesto, CA) for their assistance at the dairy; and Arlen Anderson and Wendy Johnson (USDA-ARS, Virus and Prion Diseases of Livestock Research Unit, Ames, IA) for excellent technical assistance in the bovine leukocyte adhesion deficiency genotyping. All programs and services of the USDA are offered on a nondiscriminatory basis, without regard to race, color, national origin, religion, sex, age, marital status, or handicap. No endorsements are herein implied. NR 45 TC 5 Z9 5 U1 0 U2 9 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2010 VL 88 IS 8 BP 2585 EP 2593 DI 10.2527/jas.2009-2610 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 624DQ UT WOS:000279796800005 PM 20382870 ER PT J AU Schieck, SJ Kerr, BJ Baidoo, SK Shurson, GC Johnston, LJ AF Schieck, S. J. Kerr, B. J. Baidoo, S. K. Shurson, G. C. Johnston, L. J. TI Use of crude glycerol, a biodiesel coproduct, in diets for lactating sows SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE glycerol; lactation; sow ID HIGH AMBIENT-TEMPERATURES; GROWING PIGS; PERFORMANCE; HYPERHYDRATION; EXERCISE; TIME; ACID AB An experiment was conducted to evaluate the effect of dietary crude glycerol in lactating sow diets on sow and litter performance under heat stress conditions. Mixed parity (range = 0 to 13) sows (n = 345; 253 +/- 24 kg of BW) were assigned randomly within gestation housing location and parity to 1 of 4 dietary treatments. Treatments consisted of a corn-soybean-based control diet (CON) and 3, 6, or 9% glycerol added at the expense of corn and soybean meal. Liquid crude glycerol was incorporated in the complete diet at the time of mixing. Dietary treatments were imposed on d 109 of gestation (2.25 kg/d) when sows were moved into farrowing rooms. Heat index during lactation in farrowing rooms exceeded 25 degrees C for all sows. At farrowing, sows were allowed ad libitum access to feed throughout lactation. Dietary treatment tended ( P = 0.08) to influence ADFI of sows (CON = 6.04 kg/d; 3% = 6.21 kg/d; 6% = 5.69 kg/d; 9% = 6.00 kg/d; pooled SE = 0.18). Up to 9% crude glycerol in the diet had no effect on sow BW and backfat loss, weaning-to-estrus interval, preweaning mortality of piglets, and ADG of piglets. Increasing dietary glycerol linearly reduced (P = 0.10) litter size at weaning (CON = 9.50; 3% = 9.60; 6% = 9.36; 9% = 9.39; pooled SE = 0.08). Daily water consumption was not affected by dietary treatment. Crude glycerol did not affect respiration rates or rectal body temperatures, indicating no efficacy in reducing heat stress of sows. Plasma glycerol concentrations increased linearly (P < 0.05) as dietary crude glycerol increased (CON = 1.21 mu M; 3% = 1.69 mu M; 6% = 7.21 mu M; 9% = 29.04 mu M; pooled SE = 1.58), but plasma glucose concentrations were not affected. Crude protein content of the milk of sows was not affected (P = 0.16) by dietary treatment. Dry matter (P = 0.07) and crude fat (P = 0.09) content of the milk of the sows tended to increase linearly (DM basis: CON = 17.84%; 3% = 18.43%; 6% = 18.98%; 9% = 18.48%; pooled SE = 0.34; crude fat: CON = 4.78%; 3% = 4.91%; 6% = 5.50%; 9% = 5.24%; pooled SE = 0.30), whereas milk ash concentration tended (P = 0.09) to decrease linearly with increasing dietary glycerol (CON = 0.77%; 3% = 0.79%; 6% = 0.74%; 9% = 0.74%; pooled SE = 0.02). Increasing dietary crude glycerol linearly increased ( P < 0.05) lactose concentration in the milk of sows (CON = 5.16%; 3% = 5.30%; 6% = 5.43%; 9% = 5.46%; pooled SE = 0.10). Results from this study indicate that lactating sows fed diets containing up to 9% crude glycerol perform similarly to sows fed a standard corn-soybean meal diet. C1 [Johnston, L. J.] W Cent Res & Outreach Ctr, Morris, MN 56267 USA. [Schieck, S. J.; Shurson, G. C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. [Kerr, B. J.] ARS, USDA, Ames, IA 50011 USA. [Baidoo, S. K.] So Res & Outreach Ctr, Waseca, MN 56093 USA. RP Johnston, LJ (reprint author), W Cent Res & Outreach Ctr, Morris, MN 56267 USA. EM johnstlj@morris.umn.edu FU Minnesota Pork Board; Minnesota Agricultural Experiment Station; SoyMor Biodiesel LLC, Albert Lea, MN FX This project was financially supported by the Minnesota Pork Board, the Minnesota Agricultural Experiment Station, and SoyMor Biodiesel LLC, Albert Lea, MN. NR 33 TC 16 Z9 16 U1 1 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2010 VL 88 IS 8 BP 2648 EP 2656 DI 10.2527/jas.2009-2609 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 624DQ UT WOS:000279796800012 PM 20382881 ER PT J AU Neville, TL Redmer, DA Borowicz, PP Reed, JJ Ward, MA Johnson, ML Taylor, JB Soto-Navarro, SA Vonnahme, KA Reynolds, LP Caton, JS AF Neville, T. L. Redmer, D. A. Borowicz, P. P. Reed, J. J. Ward, M. A. Johnson, M. L. Taylor, J. B. Soto-Navarro, S. A. Vonnahme, K. A. Reynolds, L. P. Caton, J. S. TI Maternal dietary restriction and selenium supply alters messenger ribonucleic acid expression of angiogenic factors in maternal intestine, mammary gland, and fetal jejunal tissues during late gestation in pregnant ewe lambs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE angiogenic factor; dietary restriction; ewe; fetus; selenium ID VISCERAL ORGAN MASS; INTRAUTERINE GROWTH-RETARDATION; BODY-WEIGHT; NUTRIENT RESTRICTION; BEEF STEERS; VASCULARITY; CELLULARITY; SHEEP; PROLIFERATION; NUTRITION AB The objectives of this study were to evaluate effects of maternal dietary restriction and Se supply on angiogenic factor mRNA expression in intestinal and mammary tissues, and jejunal crypt cell proliferation and vascularity in late-term fetal intestines. In Exp. 1, pregnant ewe lambs (n = 32; initial BW = 45.6 +/- 2.3 kg) were allotted randomly to 1 of 4 treatments. Treatments ( initiated d 50 +/- 5 of gestation) were control (3.5 mu g of Se.kg of BW-1.d(-1)), Se-wheat (75 mu g of Se.kg of BW-1.d(-1)), selenate (Se3; providing 75 mu g of Se.kg of BW-1.d(-1)), selenate (Se15; providing 375 mu g of Se.kg of BW-1.d(-1)). Diets (DM basis) were similar in CP (15.5%) and ME (2.68 Mcal/kg). In Exp. 2, pregnant ewe lambs (n = 36; initial BW 53.8 +/- 1.3 kg) were allotted randomly to treatments in a 2 x 2 factorial arrangement. Factors were nutrition ( control, 100% of requirements vs. restricted nutrition, 60% of controls) and dietary Se ( adequate Se; 6 mu g of Se.kg of BW-1.d(-1) vs. high Se; 80 mu g of Se.kg of BW-1.d(-1)). Selenium treatments were initiated 21 d before breeding, and nutritional treatments were initiated on d 64 of gestation. Diets ( DM basis) were 16% CP and 2.12 Mcal/kg of ME. In Exp. 1, Se15 increased (P = 0.07) vascular endothelial growth factor ( VEGF) mRNA expression, whereas Se supplementation decreased (P = 0.06) kinase insert domain receptor (KDR) mRNA in maternal mucosal scrape on d 134 of gestation. Expression of VEGF mRNA was decreased by Se (P = 0.10) in fetal jejunum. In mammary tissue, fms-related tyrosine kinase 1 and KDR mRNA were greater in Se-wheat compared with Se3, and KDR expression was increased (P = 0.10) in Se15 compared with Se3. In Exp. 2, dietary restriction increased (P = 0.07) expression of mRNA for VEGF, fms-related tyrosine kinase 1, KDR, neuropilin 1, neuropilin 2, and hypoxia-inducible factor 1, a subunit in mucosal scrapes from maternal jejunum. In fetal jejunum, soluble guanylate cyclase, was decreased (P = 0.01) by maternal dietary restriction from d 64 to 135 of gestation. Total microvascularity in fetal jejunum was reduced (P = 0.002) by maternal dietary restriction. Mammary gland expression of VEGF, neuropilin 1, angiopoietin receptor ( endothelial tyrosine kinase), and endothelial nitric oxide synthase 3 increased (P = 0.09), whereas angiopoietin 1 decreased (P = 0.05) due to nutrient restriction. Data indicate that expression of angiogenic factors and receptors in maternal intestine, mammary gland, and fetal jejunum are responsive to maternal nutrition and likely explain observed changes in tissue vascularity. C1 [Neville, T. L.; Redmer, D. A.; Borowicz, P. P.; Reed, J. J.; Ward, M. A.; Johnson, M. L.; Vonnahme, K. A.; Reynolds, L. P.; Caton, J. S.] N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. [Taylor, J. B.] ARS, USDA, US Sheep Expt Stn, Dubois, ID 83423 USA. [Soto-Navarro, S. A.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. RP Caton, JS (reprint author), N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. EM Joel.Caton@ndsu.edu RI Reynolds, Lawrence/I-5267-2015 OI Reynolds, Lawrence/0000-0002-6838-7809 FU USDA Cooperative State Research, Education, and Extension Service [2003-35206-13621, 2005-35206-15281]; National Institutes of Health [HL 64141]; USDA-IFAFS [00-52102-9636] FX This project was partially supported by National Research Initiative Competitive Grants No. 2003-35206-13621 and 2005-35206-15281 from the USDA Cooperative State Research, Education, and Extension Service, by National Institutes of Health Grant HL 64141, and by USDA-IFAFS Grant No. 00-52102-9636. Gratitude is expressed to employees of the Animal Nutrition and Physiology Center and Ruminant Nutrition Laboratory (Fargo, ND) for their contributions to this project. NR 49 TC 19 Z9 19 U1 0 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2010 VL 88 IS 8 BP 2692 EP 2702 DI 10.2527/jas.2009-2706 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 624DQ UT WOS:000279796800017 PM 20407071 ER PT J AU Burke, JM Coleman, SW Chase, CC Riley, DG Looper, ML Brown, MA AF Burke, J. M. Coleman, S. W. Chase, C. C., Jr. Riley, D. G. Looper, M. L. Brown, M. A. TI Interaction of breed type and endophyte-infected tall fescue on milk production and quality in beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; milk production; milk quality; tall fescue ID RECIPROCAL-CROSS COWS; HEAT-STRESS; ACREMONIUM-COENOPHIALUM; PLASMA-CONCENTRATIONS; COMMON BERMUDAGRASS; OVARIAN-FUNCTION; ESTROUS-CYCLE; BRAHMAN; ANGUS; PROLACTIN AB Decreased milk production of beef cattle grazing endophyte-infected (EI) tall fescue (TF), an important cool season grass in the southeastern United States, can affect calf growth. The objective was to determine whether a thermal or heat-tolerant Bos taurus breed of cattle, Romosinuano (RO), would tolerate EI TF toxins relative to Angus (AN) cattle. Milk production and quality were examined on AN and RO cows grazing endophyte-free (EF; variety K-31; n = 36) or EI TF (variety K-31; n = 37) along with growth of their Charolais-sired calves in 2007 and 2008 in west central Arkansas. Cows calved between early February and late March (spring). Milk yield and quality, BW, rectal temperature, and serum prolactin were determined at 57.1 +/- 2.6 d postpartum and every 28 d thereafter between April and August. Milk yield was greater in AN compared with RO cows (P < 0.001), but not influenced by forage type (P > 0.88). Percent milk fat was greater in cows grazing EF TF in April, but greater for cows grazing EI TF in July (forage x month, P < 0.001). Percent milk fat was greater for RO than AN cows (P < 0.001). Percent milk protein (P < 0.001) was greater and somatic cell counts (log-transformed; P < 0.001) were less in RO than AN cows. Milk lactose was greater for RO compared with AN cows in June through August (breed x month, P = 0.004). Adjusted weaning BW of calves was similar between EF and EI TF in 2007, but greater for calves from EF than EI TF in 2008 (forage x year, P = 0.03). Rectal temperature was similar between RO cows grazing EF and EI TF, but greater in AN cows grazing EI compared with EF TF in most months (forage x breed x month x year, P < 0.001). Serum prolactin was reduced in both breeds of cows grazing EI TF between April and July of both years and greatest in RO cows grazing EF TF (breed, P < 0.001; forage x month, P < 0.001). These data suggest that RO cows were more thermal-tolerant, but still susceptible to toxins in EI TF as shown by a reduction in serum concentrations of prolactin. However, milk production was not influenced by EI TF as previously observed, but milk fat percent was decreased in early lactation in this group of cows. Milk yield and quality were different between AN and RO cows during the period of lactation observed. C1 [Burke, J. M.; Looper, M. L.] USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Coleman, S. W.; Chase, C. C., Jr.; Riley, D. G.] USDA ARS, SubTrop Agr Res Stn, Brooksville, FL 34601 USA. [Brown, M. A.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Burke, JM (reprint author), USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. EM joan.burke@ars.usda.gov NR 33 TC 8 Z9 8 U1 0 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2010 VL 88 IS 8 BP 2802 EP 2811 DI 10.2527/jas.2009-2214 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 624DQ UT WOS:000279796800027 PM 20418449 ER PT J AU Zerzghi, H Brooks, JP Gerba, CP Pepper, IL AF Zerzghi, H. Brooks, J. P. Gerba, C. P. Pepper, I. L. TI Influence of long-term land application of Class B biosolids on soil bacterial diversity SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE 16S rRNA; bacterial diversity; biosolids; land application; long-term effects ID 16S RIBOSOMAL-RNA; SOUTHWESTERN UNITED-STATES; MICROBIAL DIVERSITY; SEWAGE-SLUDGE; PCR BIAS; COMMUNITY; CULTIVATION; POPULATIONS; SEQUENCES; LIBRARIES AB Aim: To evaluate the effect of long-term annual land applications of Class B biosolids on soil bacterial diversity at University of Arizona Marana Agricultural Field Center, Tucson, Arizona. Methods and Results: Following the final of 20 consecutive years of application of Class B biosolids in March 2005, followed by cotton growth from April to November 2005 surface soil samples (0-30 cm) were collected from control (unamended) and biosolid-amended plots. Total bacterial community DNA was extracted, amplified using 16S rRNA primers, cloned, and sequenced. All 16S rRNA sequences were identified by 16S rRNA sequence analysis and comparison to known sequences in GenBank (NCBI BlastN and Ribosomal Database Project II, RDP). Results showed that the number of known genera (identifiable > 96%) increased in the high rate biosolid plots compared to control plots. Biosolids-amended soils had a broad phylogenetic diversity comprising more than four major phyla: Proteobacteria (32%), Acidobacteria (21%), Actinobacteria (16%), Firmicutes (7%), and Bacteroidetes (6%) which were typical to bacterial diversity found in the unamended arid southwestern soils. Conclusion: Bacterial diversity was either enhanced or was not negatively impacted following 20 years of land application of Class B biosolids. Significance and Impact of the Study: This study illustrates that long-term land application of biosolids to arid southwestern desert soils has no deleterious effect on soil microbial diversity. C1 [Zerzghi, H.; Gerba, C. P.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Brooks, J. P.] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS USA. [Pepper, I. L.] Univ Arizona, Environm Res Lab, Tucson, AZ 85721 USA. RP Zerzghi, H (reprint author), Univ Arizona, Dept Soil Water & Environm Sci, 1177 E 4th St,Shantz Bldg,Room 429, Tucson, AZ 85721 USA. EM huruyg@email.arizona.edu FU National Science Foundation Water Quality Center at the University of Arizona FX This work was funded by the National Science Foundation Water Quality Center located at the University of Arizona. The authors thank Al Agellon for his technical assistance and Susan J. Miller (University of Arizona, Biotechnology Computing Facility) for her assistance in data analysis. NR 41 TC 7 Z9 7 U1 0 U2 14 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD AUG PY 2010 VL 109 IS 2 BP 698 EP 706 DI 10.1111/j.1365-2672.2010.04698.x PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 623IU UT WOS:000279733700032 PM 20202022 ER PT J AU McAuliffe, JR Hamerlynck, EP AF McAuliffe, J. R. Hamerlynck, E. P. TI Perennial plant mortality in the Sonoran and Mojave deserts in response to severe, multi-year drought SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Ambrosia; Disturbance; Larrea tridentata; Soil; SPI; Standard precipitation index ID CERCIDIUM-MICROPHYLLUM; FOUQUIERIA-SPLENDENS; ROOT COMMUNICATION; SHRUBS; DYNAMICS; TREE; PRECIPITATION; CALIFORNIA; DIEBACK; USA AB Recent drought caused considerable shrub mortality in parts of the Sonoran and Mojave deserts (USA) The most severe impacts occurred in southern parts of the Mojave Desert and the adjacent Lower Colorado section of the Sonoran Desert in southernmost California and southwestern Arizona In that region, mortality of the small, drought-deciduous shrubs Ambrosia deltoidea and Ambrosia dumosa was 100% or nearly so The larger, drought-enduring evergreen Larrea tridentata fared much better, although nearly two-thirds of all L tridentata plants succumbed at one location Data on 21 additional perennial species showed that other species of small, drought-deciduous shrubs also sustained considerably higher mortality. The best predictor of plant responses was the Standard Precipitation Index (SPI) calculated for a 60-month time frame ending in March 2003, indicating the Importance of the cumulative effect of successive drought years Deficits of cool-season precipitation were more extreme than warm-season deficits and were probably the greatest contributor to plant mortality. Soil conditions, including differences in parent materials and texture, also influenced plant responses Episodes of drought-induced, perennial plant mortality represent extensive, region-wide ecological disturbances and may be one of the most important processes affecting plant populations and community composition in deserts (C) 2010 Elsevier Ltd All rights reserved C1 [McAuliffe, J. R.] Desert Bot Garden, Phoenix, AZ 85008 USA. [Hamerlynck, E. P.] USDA ARS, SW Watershed Res Ctr, Tucson, AZ 85719 USA. RP McAuliffe, JR (reprint author), Desert Bot Garden, 1201 N Galvin Pkwy, Phoenix, AZ 85008 USA. FU NSF FX National Park Service personnel at Organ Pipe Cactus National Monument (Sue Rutman. Tim Tibbitts), Joshua Tree National Park (Christine Wilson), and Death Valley National Park (Dick Anderson) facilitated our work in those parks. Twenty-four participants of a Chautauqua short course offered by EPH and John Carothers (supported by the NSF Division of Undergraduate Education) assisted with collection of data from the Providence Mountain site Jim Andre of the Univ. of California Sweeney Granite Mountain Desert Research Center and Robert Fulton of the California State University Desert Studies Center in Zzyzx, CA facilitated fieldwork in the Mojave Desert Travis Huxman and Ed Bobich assisted with collection of data at one site in Organ Pipe Cactus National Monument and Jennifer Johnson, Matthew King, and Jacob McAuliffe assisted in collection of data in 2009. Pete Sundt, Matthew King, and three anonymous reviewers provided helpful suggestions for improving the manuscript. NR 26 TC 49 Z9 50 U1 4 U2 52 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 J9 J ARID ENVIRON JI J. Arid. Environ. PD AUG PY 2010 VL 74 IS 8 BP 885 EP 896 DI 10.1016/j.jaridenv.2010.01.001 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 609KX UT WOS:000278656400001 ER PT J AU Ippolito, JA Blecker, SW Freeman, CL McCulley, RL Blair, JM Kelly, EF AF Ippolito, J. A. Blecker, S. W. Freeman, C. L. McCulley, R. L. Blair, J. M. Kelly, E. F. TI Phosphorus biogeochemistry across a precipitation gradient in grasslands of central North America SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Grassland ecosystems; Phosphorus biogeochemistry; Sequential phosphorus extraction; Soil weathering ID AFFECTING PHOSPHATE SORPTION; GREAT-PLAINS; ORGANIC-CARBON; UNITED-STATES; SOIL-P; LANDSCAPE; DESERT; FORMS; CHRONOSEQUENCE; AVAILABILITY AB Soil P transformations and distribution studies under water limited conditions that characterize many grasslands may provide further insight into the importance of abiotic and biotic P controls within grass-dominated ecosystems We assessed transformations between P pools across four sites spanning the shortgrass steppe, mixed grass prairie, and tallgrass prairie along a 400-mm precipitation gradient across the central Great Plains Pedon total elemental and constituent mass balance analyses reflected a pattern of increased chemical weathering from the more arid shortgrass steppe to the more mesic tallgrass prairie Soil surface A horizon P accumulation was likely related to increased biocycling and biological mining. Soluble P, a small fraction of total P in surface A horizons, was greatest at the mixed grass sites The distribution of secondary soil P fractions across the gradient suggested decreasing Ca-bound P and increasing amounts of occluded P with increasing precipitation Surface A horizons contained evidence of Ca-bound P in the absence of CaCO(3), while in subsurface horizons the Ca-bound P was associated with increasing CaCO(3) content Calcium-bound P. which dominates in water-limited systems, forms under different sets of soil chemical conditions in different climatic regimes, demonstrating the importance of carbonate regulation of P in semi-arid ecosystems Published by Elsevier Ltd C1 [Ippolito, J. A.] USDA ARS, NW Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Blecker, S. W.; Freeman, C. L.; Kelly, E. F.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [McCulley, R. L.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Blair, J. M.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. RP Ippolito, JA (reprint author), USDA ARS, NW Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. RI Blair, John/I-4082-2014 OI Blair, John/0000-0003-0072-0721 NR 54 TC 8 Z9 10 U1 1 U2 27 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 J9 J ARID ENVIRON JI J. Arid. Environ. PD AUG PY 2010 VL 74 IS 8 BP 954 EP 961 DI 10.1016/j.jaridenv.2010.01.003 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 609KX UT WOS:000278656400010 ER PT J AU Lucero, ME Dreesen, DR VanLeeuwen, DM AF Lucero, M. E. Dreesen, D. R. VanLeeuwen, D. M. TI Using hydrogel filled, embedded tubes to sustain grass transplants for arid land restoration SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Black grama; Remote site irrigation; Soil Moist (TM) AB Glass restoration on remote arid rangelands may require irrigation to stimulate establishment However, irrigation on undeveloped sites is costly Vertical irrigation tubes that direct applied moisture to subsurface zones where evaporation is reduced, and hydrogels that prevent applied moisture from infiltrating beyond plant root zones can maximize the portion of applied water available for plant uptake. The survival and growth of Bouteloua eriopoda (Toir) Torr transplants irrigated with either starch- or acrylic-based hydrogels contained in one of three embedded watering tube styles were evaluated in a greenhouse trial A field trial evaluated differences in transplant survival and cover between treatments consisting of embedded watering tubes with or without acrylic hydrogels Greenhouse transplants from all treatments grew 146 days on less than 1 L of water Plains irrigated with starch hydrogels consumed the most water and exhibited the most growth Variations in tube styles had minor effects on plant growth and water loss from tubes In the field, heavy growing season precipitation was observed, and transplant survival was high for both treatments. No significant differences in cover were detected. Greenhouse data demonstrate potential for hydrogel filled, embedded tubes to provide adequate moisture for establishment and growth of deep-rooted black grama transplants Field data indicate deep-rooted black grama transplants establish successfully when adequate moisture is available Published by Elsevier Ltd C1 [Lucero, M. E.] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Dreesen, D. R.] USDA NRCS Plant Mat Ctr, Los Lunas, NM 87031 USA. [VanLeeuwen, D. M.] New Mexico State Univ, Dept Econ & Int Business, Las Cruces, NM 88003 USA. RP Lucero, ME (reprint author), USDA ARS Jornada Expt Range, 2995 Knox St, Las Cruces, NM 88003 USA. FU USDA [6235-11210-005-00D]; Bureau of Land Management; New Mexico State Office; New Mexico Agricultural Experiment Station; National Science Foundation [DEB-0618210] FX We thank Mike Howard, Bureau of Land Management, New Mexico State Office for suggesting utilization of embedded irrigation tubes for grass transplant establishment and Patrick Holcomb and Jason Dunwell for assistance with project setup and data collection This research was funded in part by the USDA-ARS Jornada Experimental Range (Project number 6235-11210-005-00D), the Bureau of Land Management, New Mexico State Office, the New Mexico Agricultural Experiment Station and USDA Cooperative State Research, Education & Extension Service, Hatch project NM-VanLeeuwen-08H and the National Science Foundation Jornada Basin Long Term Ecological Research Program (DEB-0618210) NR 13 TC 2 Z9 2 U1 1 U2 7 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 J9 J ARID ENVIRON JI J. Arid. Environ. PD AUG PY 2010 VL 74 IS 8 BP 987 EP 990 DI 10.1016/j.jaridenv.2010.01.007 PG 4 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 609KX UT WOS:000278656400014 ER PT J AU Iyer, PR Geib, SM Catchmark, J Kao, TH Tien, M AF Iyer, Prashanti R. Geib, Scott M. Catchmark, Jeff Kao, Teh-hui Tien, Ming TI Genome Sequence of a Cellulose-Producing Bacterium, Gluconacetobacter hansenii ATCC 23769 SO JOURNAL OF BACTERIOLOGY LA English DT Article ID ACETOBACTER-XYLINUM; CRYSTALLIZATION AB The Gram-negative bacterium Gluconacetobacter hansenii is considered a model organism for studying cellulose synthesis. We have determined the genome sequence of strain ATCC 23769. C1 [Tien, Ming] Penn State Univ, Dept Biochem & Mol Biol, Althouse Lab 408, University Pk, PA 16802 USA. [Iyer, Prashanti R.] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. [Geib, Scott M.] ARS, USDA, Pacific Basin Agr Res Ctr, Hilo, HI USA. [Catchmark, Jeff] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. RP Tien, M (reprint author), Penn State Univ, Dept Biochem & Mol Biol, Althouse Lab 408, University Pk, PA 16802 USA. EM mxt3@psu.edu FU U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0001090] FX This material is based upon work supported as part of the Center for LignoCellulose Structure and Function, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under award number DE-SC0001090. NR 8 TC 21 Z9 24 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD AUG PY 2010 VL 192 IS 16 BP 4256 EP 4257 DI 10.1128/JB.00588-10 PG 2 WC Microbiology SC Microbiology GA 632FL UT WOS:000280406300021 PM 20543071 ER PT J AU Denizman, C Brevik, EC Doolittle, J AF Denizman, Can Brevik, Eric C. Doolittle, Jim TI GROUND-PENETRATING RADAR INVESTIGATION OF A RAPIDLY DEVELOPED SMALL ISLAND IN A LAKE IN SOUTHERN GEORGIA, USA SO JOURNAL OF CAVE AND KARST STUDIES LA English DT Article ID NORTH-CENTRAL FLORIDA; SEDIMENTS; CARBONATE; EVOLUTION; IGNITION; PONDS AB Collapse sinkholes commonly form in karstic limestones of the Floridan aquifer of southern Georgia, USA. The limestones are capped by impermeable strata that can obscure developing sub-surface voids and catastrophically collapse when too much of their underlying support has been removed. We investigated the overnight appearance of an island in a Georgian lake and its possible relationship to the underlying Floridan aquifer using ground-penetrating radar and global-positioning-satellite spot elevations. The island is adjacent to a submerged sinkhole with an arcuate depression and whose development included convergent downward slumping. Compression created by the convergence probably squeezed lake-bottom sediments upward to form the unusual island. Our methodology can be applied to other lakes in karst regions and may prove useful for diagnosing existing or future subsidence risks. C1 [Brevik, Eric C.] Dickinson State Univ, Dept Nat Sci & Agr, Dickinson, ND 58601 USA. [Brevik, Eric C.] Dickinson State Univ, Dept Tech Studies, Dickinson, ND 58601 USA. [Denizman, Can] Valdosta State Univ, Dept Phys Astron & Geosci, Valdosta, GA 31698 USA. [Doolittle, Jim] USDA NRCS NSSC, Newtown Sq, PA 19073 USA. RP Brevik, EC (reprint author), Dickinson State Univ, Dept Nat Sci & Agr, Dickinson, ND 58601 USA. EM cdenizma@valdosta.edu; Eric.Brevik@dsu.nodak.edu; jim.doolittle@lin.usda.gov OI Brevik, Eric/0000-0002-6004-0018 NR 23 TC 3 Z9 3 U1 0 U2 7 PU NATL SPELEOLOGICAL SOC PI HUNTSVILLE PA 2813 CAVE AVE, HUNTSVILLE, AL 35810-4431 USA SN 1090-6924 J9 J CAVE KARST STUD JI J. Cave Karst Stud. PD AUG PY 2010 VL 72 IS 2 BP 94 EP 99 DI 10.4311/jcks2008es0060 PG 6 WC Geosciences, Multidisciplinary SC Geology GA 646XO UT WOS:000281578600004 ER PT J AU Diedrich, CG Copeland, JP AF Diedrich, Cajus G. Copeland, Jeffrey P. TI UPPER PLEISTOCENE GULO GULO (LINNE, 1758) REMAINS FROM THE SRBSKO CHLUM-KOMIN HYENA DEN CAVE IN THE BOHEMIAN KARST, CZECH REPUBLIC, WITH COMPARISONS TO CONTEMPORARY WOLVERINES SO JOURNAL OF CAVE AND KARST STUDIES LA English DT Article ID SPOTTED HYENAS; SURVIVAL; SITES AB Wolverine bone material is described from the famous Upper Pleistocene cave Srbsko Chlum-Komin in the Bohemian Karst, Czech Republic, along with an overview of recently known Czech sites. The Gulo gulo Linne material was found in one of the largest Ice Age spotted-hyena dens in Europe. As a result of non-systematic excavations, the taphonomy is partly unclear. Lower-jaw remains indicate a minimum of three wolverines. Two of the mandibles are cracked, which is most likely the result of carnivore scavenging. The absence of juvenile G. gulo suggests possible importation of the wolverines by hyenas Crocuta crocuta spelaea Goldfuss. C1 [Diedrich, Cajus G.] PaleoLogic, D-33790 Halle, Germany. [Copeland, Jeffrey P.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Diedrich, CG (reprint author), PaleoLogic, Nansenstr 8, D-33790 Halle, Germany. EM cdiedri@gmx.net; jpcopeland@fs.fed.us NR 21 TC 1 Z9 1 U1 0 U2 8 PU NATL SPELEOLOGICAL SOC PI HUNTSVILLE PA 2813 CAVE AVE, HUNTSVILLE, AL 35810-4431 USA SN 1090-6924 J9 J CAVE KARST STUD JI J. Cave Karst Stud. PD AUG PY 2010 VL 72 IS 2 BP 122 EP 127 DI 10.4311/jcks2008pa0070 PG 6 WC Geosciences, Multidisciplinary SC Geology GA 646XO UT WOS:000281578600008 ER PT J AU Schnupf, U Willett, JL Momany, FA AF Schnupf, Udo Willett, J. L. Momany, Frank A. TI 27 ps DFT Molecular Dynamics Simulation of alpha-Maltose: A Reduced Basis Set Study SO JOURNAL OF COMPUTATIONAL CHEMISTRY LA English DT Article DE B3LYP/6-31+G*; 4-31G; DFTMD; dynamics; maltose ID BETA-D-GLUCOPYRANOSE; B3LYP/6-311++G-ASTERISK-ASTERISK LEVEL; WATER-MOLECULES; FORCE-FIELD; GEOMETRY-OPTIMIZATION; COMPUTER EXPERIMENTS; CRYSTAL-STRUCTURE; CLASSICAL FLUIDS; AQUEOUS-SOLUTION; ENERGY SURFACES AB DFT molecular dynamics simulations are time intensive when carried out on carbohydrates such as alpha-maltose In a recent publication (Momany et al.. J. Mol. Struct THEOCHEM. submitted) forces for dynamics were generated from B3LYP/6-31 +G* electronic structure calculations The implicit solvent method COSMO was applied to simulate the solution environment Here we present a modification of the DFT method that keeps the critical aspects of the larger basis set (B3LYP/6-31 +G*) while allowing the less-essential atom interactions to be calculated using a mallet basis set, thus allowing for faster completion without sacrificing the interactions dictating the hydrogen bonding networks in alpha-maltose In previous studies, the gg'-gg-c solvated form quickly converged to the "r" form during a 5 ps dynamics run This important conformational transition is tested by carrying out a long 27 ps simulation. The trend for the "r" conformer to be most stable during dynamics when fully solvated, is confirmed, resulting in similar to 20/80% c/r population Further, the study shows that considerable molecular end effects are important, the reducing end being fairly stable. the O6-H pointing at the O5. while the nonreducing end moves freely to take on different conformations Some "kink" and transition state forms are populated during the simulation The average H1' H4 distance of 2 28 angstrom confirms that the syn form is the primary glycosidic conformation, while the average C1'-O1'-C4 bond angle was 118 8 degrees, in excellent agreement with experimental values. The length of this simulation allowed the evaluation of vibrational frequencies by Fourier transform of the velocity correlation function, taken from different time segments along the simulation path C1 [Schnupf, Udo; Willett, J. L.; Momany, Frank A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Momany, FA (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RI Schnupf, Udo/H-4703-2016 OI Schnupf, Udo/0000-0002-1457-1985 NR 61 TC 6 Z9 6 U1 1 U2 13 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0192-8651 J9 J COMPUT CHEM JI J. Comput. Chem. PD AUG PY 2010 VL 31 IS 11 BP 2087 EP 2097 DI 10.1002/jcc.21495 PG 11 WC Chemistry, Multidisciplinary SC Chemistry GA 620PG UT WOS:000279511200001 PM 20127742 ER PT J AU Smith, RL Strawderman, RL Schukken, YH Wells, SJ Pradhan, AK Espejo, LA Whitlock, RH Van Kessel, JS Smith, JM Wolfgang, DR Grohn, YT AF Smith, R. L. Strawderman, R. L. Schukken, Y. H. Wells, S. J. Pradhan, A. K. Espejo, L. A. Whitlock, R. H. Van Kessel, J. S. Smith, J. M. Wolfgang, D. R. Groehn, Y. T. TI Effect of Johne's disease status on reproduction and culling in dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE Johne's disease; reproduction; culling; survival analysis ID AVIUM SUBSP PARATUBERCULOSIS; MYCOBACTERIUM-PARATUBERCULOSIS; MILK-PRODUCTION; RECURRENT EVENTS; ENERGY-BALANCE; FECAL SAMPLES; COWS; CULTURE; HERDS; REGRESSION AB Among the costs attributed to Mycobacterium avium ssp. paratuberculosis (MAP) infection in dairy cattle, the effects on reproduction and culling are the least documented. To estimate the cost of MAP infections and Johne's disease in a dairy herd, the rates of calving and culling were calculated for cows in each stage of MAP infection relative to uninfected cows. Data from 6 commercial dairy herds, consisting of 2,818 cows with 2,754 calvings and 1,483 cullings, were used for analysis. Every cow in each study herd was tested regularly for MAP, and herds were followed for between 4 and 7 yr. An ordinal categorical variable for Johne's disease status [test-negative, low-positive (low-shedding or ELISA-positive only), or high-shedding] was defined as a time-dependent variable for all cows with at least 1 positive test result or 2 negative test results. A Cox regression model, stratified on herd and controlling for the time-dependent infection variable, was used to analyze time to culling. Nonshedding animals were significantly less likely to be culled in comparison with animals in the low-shedding or ELISA-positive category, and high-shedding animals had nonsignificantly higher culling rates than low-shedding or ELISA-positive animals. Time to calving was analyzed using a proportional rates model, an analog to the Andersen-Gill regression model suitable for recurrent event data, stratifying on herd and weighted to adjust for the dependent censoring caused by the culling effects described above. High-shedding animals had lower calving rates in comparison with low-shedding or ELISA-positive animals, which tended to have higher calving rates than test-negative animals. C1 [Smith, R. L.; Groehn, Y. T.] Cornell Univ, Epidemiol Sect, Dept Populat Med & Diag Sci, Coll Vet Med, Ithaca, NY 14850 USA. [Strawderman, R. L.] Cornell Univ, Dept Biol Stat & Computat Biol, Ithaca, NY 14850 USA. [Schukken, Y. H.; Pradhan, A. K.] Cornell Univ, Qual Milk Prod Serv, Dept Populat Med & Diag Sci, Coll Vet Med, Ithaca, NY 14850 USA. [Wells, S. J.; Espejo, L. A.] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. [Whitlock, R. H.] Univ Penn, Sch Vet Med, Dept Clin Studies, New Bolton Ctr, Kennett Sq, PA 19348 USA. [Van Kessel, J. S.] ARS, Environm Microbial & Food Safety Lab, ANRI, USDA, Beltsville, MD 20705 USA. [Smith, J. M.] Univ Vermont, Dept Anim Sci, Burlington, VT 05405 USA. [Wolfgang, D. R.] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. RP Smith, RL (reprint author), Cornell Univ, Epidemiol Sect, Dept Populat Med & Diag Sci, Coll Vet Med, Ithaca, NY 14850 USA. EM rls57@cornell.edu RI Schukken, Ynte/C-3405-2008; OI Schukken, Ynte/0000-0002-8250-4194; Smith, Rebecca/0000-0002-8343-794X FU USDA (Cooperative State Research, Education and Extension Service, Washington, DC) [2008-35204-04627]; USDA-Agricultural Research Service [58-1265-3-155, 58-1265-3-156, 58-1265-3-158, 58-1265-4-020]; University Park, PA; USDA [45105] FX The USDA (Cooperative State Research, Education and Extension Service, Washington, DC) Award Number 2008-35204-04627 provided funding for this study, as did the USDA-Agricultural Research Service (Agreements 58-1265-3-155, 58-1265-3-156, 58-1265-3-158, and 58-1265-4-020) for the Regional Dairy Quality Management Alliance (RDQMA; Beltsville, MD) and the Johne's Disease Integrated Program (JDIP; University Park, PA; USDA contract 45105). NR 38 TC 29 Z9 30 U1 2 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2010 VL 93 IS 8 BP 3513 EP 3524 DI 10.3168/jds.2009-2742 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 629MN UT WOS:000280203400015 PM 20655419 ER PT J AU Gregorini, P Soder, KJ Waghorn, G AF Gregorini, P. Soder, K. J. Waghorn, G. TI Effects of timing of corn silage supplementation on digestion, fermentation pattern, and nutrient flow during continuous culture fermentation of a short and intensive orchardgrass meal SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE corn silage; supplementation; herbage digestion; nutrient supply ID DAIRY-COWS; RUMINAL PH; GRAZING BEHAVIOR; MILK-PRODUCTION; PASTURE INTAKE; NEW-ZEALAND; TIME; RUMEN; RYEGRASS; NITROGEN AB Using a dual-flow continuous culture fermenter system, this study evaluated the effect of timing of corn silage supplementation on ruminal digestion and nutrient flows following a short and intensive orchardgrass herbage meal. Treatments included 28 g dry matter (DM) of corn silage added either 9 h (9BH; 0700 h) or 1 h (1BH; 1500 h) before adding 42 g DM orchardgrass herbage or no corn silage (control; 70 g DM herbage). Herbage was fed as follows: 66% of the total herbage meal at 1600 h, 22% at 1720 h, and the remaining 12% at 1840 h. Effluent was analyzed for organic matter (OM), crude protein (CP), and neutral detergent fiber (NDF). Purine concentrations in effluent and bacterial isolates were used to estimate the partition of effluent N flow into bacterial and nonbacterial fractions, and to calculate true OM digestibility. Fermenters were sampled for pH, volatile fatty acids (VFA), and NH(3)-N at 0730, 1100, 1530, 1600, 1720, 1840, and 2000 h on d 10. Data were analyzed as a 3 x 4 Latin square experimental design. True digestibilities for OM (average of 78.5%) and CP (average of 84.6%), and apparent NDF digestibility (average of 82.7%) were not affected by treatment. Mean ruminal pH was lower for 9BH than for 1BH, averaging 5.6 and 6.5, respectively. Molar proportions of acetate were not affected by treatment. Propionate concentration was greater for 9BH than for 1BH, averaging 20.5 and 18.1 mM, respectively. Diurnal patterns of pH, NH(3)-N, and acetate: propionate ratio were affected by treatment: 9BH had the lowest values for all measurements as the day progressed. The NH(3)-N concentration and effluent NH(3)-N flow were higher for 1BH (11.4 mg/100 mL and 0.26 g/d, respectively) than for 9BH (8.8 mg/100 mL and 0.20 g/d, respectively). Effluent NH(3)-N flow (as a % of total N flow) was the lowest for 9BH. Bacterial efficiency was not affected by treatments, with a mean of 10.5 g of N/kg of OM truly digested. Under the same resource allocation (pasture plus supplement), a simple change in timing of corn silage feeding (9 rather than 1 h before an orchardgrass herbage meal) may alter ruminal fermentation pattern. These changes could increase the glucogenic nutrient supply and improve N utilization by reducing ammonia N losses. C1 [Gregorini, P.; Waghorn, G.] DairyNZ Ltd, Hamilton, New Zealand. [Gregorini, P.; Soder, K. J.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Gregorini, P (reprint author), DairyNZ Ltd, Private Bag 3221, Hamilton, New Zealand. EM Pablo.Gregorini@dairynz.co.nz OI Gregorini, Pablo/0000-0002-7084-5223 NR 43 TC 8 Z9 9 U1 0 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2010 VL 93 IS 8 BP 3722 EP 3729 DI 10.3168/jds.2009-2950 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 629MN UT WOS:000280203400037 PM 20655441 ER PT J AU Norman, HD Hutchison, JL Miller, RH AF Norman, H. D. Hutchison, J. L. Miller, R. H. TI Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holsteins in the United States SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE sexed semen; conception rate; dystocia; stillbirth ID SORTED SPERM; HEIFERS; CATTLE; INSEMINATION; COWS; FERTILITY; IMPACTS; BIRTHS; CALVES; HERDS AB Use of sexed semen for artificial insemination of US Holstein heifers (1.3 million breedings) and cows (10.8 million breedings) in Dairy Herd Improvement herds was characterized by breeding year, parity, service number, region, herd size, and herd milk yield. Sexed semen was used for 1.4, 9.5, and 17.8% of all reported breedings for 2006, 2007, and 2008, respectively, for heifers, and for 0.1, 0.2, and 0.4%, respectively, for cows. For 2008 sexed semen breedings, 80.5 and 68.6% of use was for first services of heifers and cows, respectively. For cows, 63.1% of 2008 sexed semen use was for first parity. Mean sexed semen use within herd was the greatest for heifers in the Southwest (36.2%) and for cows in the Mideast (1.3%). Mean sexed semen use increased for heifers but changed little for cows as either herd size or herd mean milk yield increased. Availability of sexed semen was examined for Holstein bulls in active AI service; of 700 bulls born after 1993, 37% had sexed semen marketed by mid August 2009. Active AI bulls with marketed sexed semen were superior to average active AI bulls for evaluations of yield traits, productive life, somatic cell score, daughter pregnancy rate, service-sire calving ease, service-sire stillbirth, final score, sire conception rate, and lifetime net merit. The effect of sexed semen use on conception rate, calf sex, dystocia, and stillbirth also was examined for heifers and cows. Mean conception rate for heifers was 56% for conventional and 39% for sexed semen; corresponding conception rates for cows were 30 and 25%. For single births from sexed semen breedings, around 90% were female. Dystocia and stillbirth were more frequent for heifers (6.0 and 10.4%, respectively, for conventional semen; 4.3 and 11.3%, respectively, for sexed semen) than for cows (2.5 and 3.6%, respectively, for conventional semen; 0.9 and 2.7%, respectively, for sexed semen). Difficult births declined by 28% for heifers and 64% for cows with sexed semen use. Stillbirths were more prevalent for twin births except for sexed semen heifer breedings. Stillbirths of single male calves of heifers were more frequent for breedings with sexed semen (15.6%) than conventional semen (10.8%); a comparable difference was not observed for cows, for which stillbirth frequency of single male calves even decreased (2.6 vs. 3.6%). Overall stillbirth frequency was reduced by sexed semen use for cows but not for heifers. C1 [Norman, H. D.; Hutchison, J. L.; Miller, R. H.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. RP Norman, HD (reprint author), ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. EM Duane.Norman@ars.usda.gov NR 31 TC 47 Z9 48 U1 1 U2 21 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2010 VL 93 IS 8 BP 3880 EP 3890 DI 10.3168/jds.2009-2781 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 629MN UT WOS:000280203400053 PM 20655457 ER PT J AU Wistrom, C Sisterson, MS Pryor, MP Hashim-Buckey, JM Daane, KM AF Wistrom, Christina Sisterson, Mark S. Pryor, Murray P. Hashim-Buckey, Jennifer M. Daane, Kent M. TI Distribution of Glassy-Winged Sharpshooter and Threecornered Alfalfa Hopper on Plant Hosts in the San Joaquin Valley, California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Pierce's disease; vector control; citrus; grape; alternate host plants ID HOMALODISCA-COAGULATA HEMIPTERA; CHAIN-REACTION PROTOCOL; XYLELLA-FASTIDIOSA; VITRIPENNIS HEMIPTERA; SOUTHERN CALIFORNIA; ORANGE TREES; CICADELLIDAE; TRANSMISSION; MANAGEMENT; DISEASE AB Homalodisca vitripennis (Cermar) and Spissistilus festinus (Say) populations were surveyed bimonthly for 14 mo in Kern County, CA, at five agricultural sites made up of a variety of potential host plants. In addition, S. festinus populations were surveyed in four alfalfa, Medicago saliva L., fields in Kern and Tulare counties. Insects were collected by beats-sweeps and sticky traps. Data on host plant condition and phenology, and ground cover presence and composition were collected at the five agricultural sites, whereas data on mowing and insecticide use were collected at the four alfalfa sites. Populations of both insects persisted at the five agricultural locations despite insecticide applications applied as part of a H. vitripennis areawide management program and standard commercial operations. Plants colonized by H. vitripennis included eucalyptus (Eucalyptus L'Her.), jojoba [Simmondsia chinensis (Link) C. K. Schneid.], and citrus (Citrus spp.). Populations of S. festinus were much greater in collections from alfalfa fields than from the five agricultural sites. Insects collected from the five mixed agricultural sites were negative for presence of X. fastidiosa. In laboratory tests, S. festinus did not acquire or transmit X. fastidiosa in tests with infected grape (Vitis spp.) as an acquisition source and grape, almond [Prunus dulcis (Mill.) D.A.Webb], and alfalfa as inoculation hosts. Recommendations for vector control, vegetation management, and targeted monitoring to reduce insect populations and inoculum potential are discussed. C1 [Wistrom, Christina; Pryor, Murray P.; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Sisterson, Mark S.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Pryor, Murray P.; Hashim-Buckey, Jennifer M.] Univ Calif Cooperat Extens, Bakersfield, CA 93307 USA. RP Daane, KM (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM daane@uckac.edu FU Almond Board of California; California Department of Food and Agriculture's Pierce's Disease/Glassy-Winged Sharpshooter Board; University of California Pierce's Disease FX We thank Marta Yamamoto for laboratory assistance, Rodrigo Almeida for advice and laboratory space, Elaine Backus for the S. festinus colony, Donal Dwyer for sampling alfalfa fields, and Foundation Plant Service at the University of California-Davis for the grapevines. Funding for this project was provided by a grant from the Almond Board of California, the California Department of Food and Agriculture's Pierce's Disease/Glassy-Winged Sharpshooter Board, and the University of California Pierce's Disease Grant Program (USDA-CSREES). NR 39 TC 4 Z9 4 U1 3 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1051 EP 1059 DI 10.1603/EC09321 PG 9 WC Entomology SC Entomology GA 634FU UT WOS:000280566500005 PM 20857711 ER PT J AU Munyaneza, JE Fisher, TW Sengoda, VG Garczynski, SF Nissinen, A Lemmetty, A AF Munyaneza, Joseph E. Fisher, Tonja W. Sengoda, Venkatesan G. Garczynski, Stephen F. Nissinen, Anne Lemmetty, Anne TI Association of "Candidatus Liberibacter solanacearum" With the Psyllid, Trioza apicalis (Hemiptera: Triozidae) in Europe SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Trioza apicalis; psyllid; carrot; liberibacter; plant pathogen ID ZEBRA CHIP DISEASE; BACTERICERA-COCKERELLI SULC; 1ST REPORT; GREENING DISEASE; SOLANUM-TUBEROSUM; POTATO-TUBERS; UNITED-STATES; NORTH-AMERICA; PEAR DECLINE; SAO-PAULO AB The psyllid Trioza apicalis Forster (Hemiptera: Triozidae) is a serious pest of carrots, Daucus carota L., in Europe. Carrots exhibiting symptoms of psyllid damage were observed in commercial fields in southern Finland in 2008. Symptoms in affected plants included leaf curling, yellow and purple discoloration of leaves, stunted growth of shoots and roots, and proliferation of secondary roots. Mechanisms by which T. apicalis induces symptoms in plants are not understood, and no plant pathogens have yet been associated with this insect. Given recent association of liberibacter with several crops affected by psyllids, an investigation on whether this bacterium is associated with T. apicalis was conducted. Polymerase chain reaction (PCR) primer pairs OA2/OI2c and LsoF/OI2c, specific for 16S rRNA gene from ''Candidatus Liberibacter solanacearum," generated amplicons of 1,168 bp and 1,173 bp, respectively, from DNA extracted from field-collected psyllids (61 and 36.6%, respectively), laboratory-reared psyllids (70 and 33.3%, respectively), field-collected petioles from symptomatic carrots (80 and 55%, respectively), and laboratory-grown carrots (100% for both primer pairs). In contrast, no PCR products were detected in DNA extracted from insect-free plants. The DNA sequences of amplicons of the genes encoding liberibacter 16S rRNA from psyllids and carrots were identical. DNA of the 16S rRNA gene sequences determined from carrots and psyllids were 99.9% identical to analogous sequences of "Ca. L. solanacearum" amplified from several solanaceous crops and the psyllid Bactericera cockerelli (Sulc), a vector of this bacterium. This is the first report of a plant pathogen associated with T. apicalis and the second known psyllid species associated with "Ca. L. solanacearum." C1 [Munyaneza, Joseph E.; Fisher, Tonja W.; Sengoda, Venkatesan G.; Garczynski, Stephen F.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Nissinen, Anne; Lemmetty, Anne] MTT Agrifood Res Finland, Plant Protect, FI-31600 Jokioinen, Finland. RP Munyaneza, JE (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM joseph.munyaneza@ars.usda.gov FU Frito Lay, Inc.; USDA-ARS State Cooperative; Texas Department of Agriculture FX We thank Jeremy L. Buchman, Millie Heidt, Peng Luo, Sakari Raiskio, and Senja Rasanen for invaluable technical assistance. We are very grateful to collaborator Finnish carrot growers. We are also thankful to anonymous reviewers who made suggestions to an earlier draft of this manuscript. Financial support for this work was partially provided by Frito Lay, Inc.; the USDA-ARS State Cooperative Potato Research Program; and Texas Department of Agriculture. NR 76 TC 58 Z9 62 U1 1 U2 20 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1060 EP 1070 DI 10.1603/EC10027 PG 11 WC Entomology SC Entomology GA 634FU UT WOS:000280566500006 PM 20857712 ER PT J AU Ulyshen, MD Duan, JJ Bauer, LS Fraser, I AF Ulyshen, Michael D. Duan, Jian J. Bauer, Leah S. Fraser, Ivich TI Suitability and Accessibility of Immature Agrilus planipennis (Coleoptera: Buprestidae) Stages to Tetrastichus planipennisi (Hymenoptera: Eulophidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE classical biological control; concealed host; exotic; forest pest; non-native ID EMERALD ASH BORER; NATURAL ENEMIES; CHINA; PARASITOIDS AB Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae), a gregarious larval endo-parasitoid, is one of three biocontrol agents from Asia currently being released in the United States to combat the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae). The current protocol for rearing T. planipennisi involves presenting the wasps with artificially infested ash sticks made by placing field-collected larvae into shallow grooves beneath flaps of bark. Although third and fourth instars are readily accepted by T. planipennisi in these exposures, the suitability of younger or older developmental stages, which are often more readily available in the field, has not been tested. In this study, we used both artificially infested ash sticks and naturally infested ash logs to test which emerald ash borer developmental stages (second to fourth instars, J larvae [preprepupae 1, prepupae, and pupae) are most suitable for rearing T. planipennisi. T. planipennisi parasitized all stages except for pupae, but parasitized fewer J larvae and prepupae in naturally infested logs than in artificially infested ash sticks. This is probably because, in naturally infested ash logs, these stages were confined to pupal chambers excavated in the sapwood and may have been largely beyond the reach of ovipositing T. planipennisi. The number of T. planipennisi progeny produced was positively correlated (logarithmic) with host weight, but this relationship was stronger when J larvae and prepupae were excluded from the data set. Fourth instars yielded the most parasitoid progeny, followed by, in approximately equal numbers, J larvae, prepupae, and third instars. Second instars yielded too few parasitoid progeny to benefit rearing efforts. C1 [Duan, Jian J.] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Ulyshen, Michael D.; Bauer, Leah S.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Bauer, Leah S.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. [Fraser, Ivich] USDA APHIS CPHST, Brighton, MI 48116 USA. RP Duan, JJ (reprint author), USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov NR 17 TC 21 Z9 23 U1 5 U2 11 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1080 EP 1085 DI 10.1603/EC10024 PG 6 WC Entomology SC Entomology GA 634FU UT WOS:000280566500008 PM 20857714 ER PT J AU Hallman, GJ Thomas, DB AF Hallman, Guy J. Thomas, Donald B. TI Ionizing Radiation as a Phytosanitary Treatment Against Fruit Flies (Diptera: Tephritidae): Efficacy in Naturally Versus Artificially Infested Fruit SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Anastrepha ludens; quarantine; commodity treatment; radiation; disinfestation ID FLY DIPTERA; QUARANTINE TREATMENT; GAMMA-RADIATION; IRRADIATION; MEXICAN AB Some phytosanitary irradiation treatment research against tephritid fruit flies (Diptera: Tephritidae) has used artificially infested fruit with the unstated and untested assumption that the method adequately simulated a natural situation. We compare grapefruit, Citrus paradisi Macfayden, naturally infested by Mexican fruit fly, Anastrepha ludens (Loew), via oviposition until larvae reached the late third instar versus insertion of diet-reared third instars into holes made in grapefruits 24 h before irradiation; the latter technique has been used in other studies. Both infestation techniques resulted in statistically indistinguishable results, indicating that insertion of diet-reared third instar Mexican fruit fly into holes bored into grapefruit and subsequently sealed 24 h before irradiation would adequately represent natural infestation and could be used to develop a radiation phytosanitary treatment of the insect in grapefruit when prevention of adult emergence is used as the measure of efficacy. Nevertheless, it may not be advisable to extend this conclusion to other fruit fly/fruit combinations without doing appropriate comparison studies. Dissection of puparia from nonirradiated control insects that failed to emerge as adults showed a relatively even distribution of mortality among the developmental stages within the puparium. In contrast, dissection of puparia from irradiated third instars that did not emerge as adults revealed a sharp attenuation in development from cryptocephalic to phanerocephalic pupae demonstrating this transition to be the developmental step most affected by radiation. C1 [Hallman, Guy J.; Thomas, Donald B.] USDA ARS, Weslaco, TX 78596 USA. RP Hallman, GJ (reprint author), USDA ARS, 2413 E Highway 83, Weslaco, TX 78596 USA. EM guy.hallman@ars.usda.gov NR 18 TC 11 Z9 11 U1 5 U2 10 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1129 EP 1134 DI 10.1603/EC09438 PG 6 WC Entomology SC Entomology GA 634FU UT WOS:000280566500013 PM 20857719 ER PT J AU Mankin, RW Moore, A AF Mankin, R. W. Moore, A. TI Acoustic Detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in Palm Trees in Urban Guam SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE invasive species; acoustic detection; eradication ID WHITE GRUBS; BEETLE; SOIL; VIBRATIONS; INFESTATIONS; LEAFMINER; PATTERNS; BEHAVIOR AB Adult and larval Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae: Dynastinae) were acoustically detected in live and dead palm trees and logs in recently invaded areas of Guam, along with Nasutitermes luzonicus Oshima (Isoptera: Termitidae), and other small, sound-producing invertebrates and invertebrates. The low-frequency, long-duration sound-impulse trains produced by large, active O. rhinoceros and the higher frequency, shorter impulse trains produced by feeding N. luzonicus had distinctive spectral and temporal patterns that facilitated their identification and discrimination from background noise, as well as from roaches, earwigs, and other small sound-producing organisms present in the trees and logs The distinctiveness of the O. rhinoceros sounds enables current usage of acoustic detection as a tactic in Guam's ongoing O. rhinoceros eradication program. C1 [Mankin, R. W.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Moore, A.] Univ Guam, Coll Nat & Appl Sci, Mangilao, GU 96923 USA. RP Mankin, RW (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM richard.mankin@ars.usda.gov FU Western Integrated Pest Management Center Special Issues Grant FX Robert Bourgeois (University of Guam), Roland Quitugua (Guam Coconut Rhinoceros Beetle Eradication Project), Everett Foreman (ARS), and Betty Weaver (ARS) provided technical support and also participated at different times as listeners on site or during subsequent playbacks of recordings in Raven along with R.W.M. and A.M. Jan Krecek and Rudolf Scheffrahn (Ft. Lauderdale Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida) provided identifications of termite specimens. Financial support was provided in part by a Western Integrated Pest Management Center Special Issues Grant. NR 36 TC 7 Z9 10 U1 0 U2 5 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1135 EP 1143 DI 10.1603/EC09214 PG 9 WC Entomology SC Entomology GA 634FU UT WOS:000280566500014 PM 20857720 ER PT J AU Ugine, TA Sensenbach, EJ Sanderson, JP Wraight, SP AF Ugine, Todd A. Sensenbach, Emily J. Sanderson, John P. Wraight, Stephen P. TI Biology and Feeding Requirements of Larval Hunter Flies Coenosia attenuata (Diptera: Muscidae) Reared on Larvae of the Fungus Gnat Bradysia impatiens (Diptera: Sciaridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biocontrol; predator; Coenosia attenuata; Bradysia; development ID CANNIBALISM; EARTHWORMS; PREY; FLY AB The larval feeding requirements and biology of the generalist predatory muscid hunter fly Coenosia attenuata Stein 1903 (Diptera: Muscidae) were investigated at 25 degrees C. Larval C. attenuata were fed second-, third, and fourth-instar (L(2), L(3), and L(4)) larvae of the fungus gnat Bradysia impatiens (Johannsen) (Diptera: Sciaridae) at variable rates to determine minimum and optimum numbers of these prey required for normal development. The proportion of C. attenuata larvae surviving to pupation differed significantly as a function of L(2) and L(3) prey numbers. When the number of prey/d was increased from 10 to 15 L(2) and from 5 to 7 L(3) per day, the respective percentages of pupation increased from 0 to 77% and from 0 to 48%. In contrast, all numbers of L(4) prey (1-7 prey per d) supported pupation, and the pupation rate did not vary with prey number. At the highest prey numbers tested, mortalities of C. attenuata larvae fed L(2), L(3), and L(4) fungus gnat larvae were 7,30, and 75%, respectively. The higher mortality of larvae fed L, prey was clearly the result of lethal wounds inflicted by the fungus gnat larvae in defensive strikes against the predators. At prey numbers supporting maximum rates of adult emergence, larval development required 12-14 d, and duration of the pupal stage was approximate to 10 d. C. attenuata larvae killed large numbers of prey during their development (means of up to 232 L(2),144 L(3), or 87 L(4) fungus gnats), and larvae provided with marginally inadequate numbers of prey survived for long periods (mean 14-22 d, maximum 34 d) before succumbing to apparent starvation. These are favorable attributes with respect to use of C. attenuata as a biological control agent, suggesting a strong potential to substantially impact high-density pest populations and to survive in low-density pest populations. C1 [Ugine, Todd A.; Sensenbach, Emily J.; Sanderson, John P.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Wraight, Stephen P.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Ugine, TA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM todd.ugine@gmail.com FU USDA-ARS Biological Integrated Pest Management Research Unit; Cornell University Department of Entomology, Ithaca, NY [58-1907-4-447]; USDA-ARS, as part of the Floriculture; Nursery Research Initiative FX This research was funded in part through a Specific Cooperative Agreement between the USDA-ARS Biological Integrated Pest Management Research Unit and the Cornell University Department of Entomology, Ithaca, NY (Specific Cooperative Agreement 58-1907-4-447) funded by the USDA-ARS, as part of the Floriculture and Nursery Research Initiative. NR 26 TC 6 Z9 6 U1 0 U2 10 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1149 EP 1158 DI 10.1603/EC09384 PG 10 WC Entomology SC Entomology GA 634FU UT WOS:000280566500016 PM 20857722 ER PT J AU Showler, AT Castro, BA AF Showler, Allan T. Castro, Boris A. TI Mexican Rice Borer (Lepidoptera: Crambidae) Oviposition Site Selection Stimuli on Sugarcane, and Potential Field Applications SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE oviposition; host plant selection; mechanism; preference; resistance ID EOREUMA-LOFTINI LEPIDOPTERA; RIO-GRANDE VALLEY; DIATRAEA-SACCHARALIS; TEXAS SUGARCANE; PYRALIDAE; CORN; RESISTANCE; PREFERENCE; LINEOLATA; PHENOLOGY AB Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), a key pest of sugarcane (Saccharum spp.) and rice, Oryza sativa L., in Texas, has not been controlled with chemical insecticides or biological agents, but some sugarcane varieties have shown degrees of resistance. Assessment of selected sugarcane leaf characteristics indicate that preference for oviposition sites is mostly determined by the presence of a leaf fold and secondarily by the availability of dry leaf tissue, both of which are antixenotic nonchemical stimuli. We suggest that breeding sugarcane lines bearing leaves that do not fold on drying could provide substantial antixenotic resistance against the Mexican rice borer. Previously identified antixenotic chemical stimuli, i.e., low quantities or absence of important nutrients in green leaf tissue, only become apparent when resistant and susceptible sugarcane varieties are compared. Varietal differences in oviposition preference, however, were not observed on excised dry leaf tissue, indicating that expression of resistance in terms of chemical stimuli requires detection of biochemicals in nearby living leaf tissue. Excised dry sugarcane leaves retain the two dominant nonchemical oviposition preference stimuli for Mexican rice borers, and the leaves effectively trapped eggs away from intact plants when dry leaves were used as "mulch" at the bottom of greenhouse cages. Under commercial sugarcane field conditions, bundled dry leaves also collected Mexican rice borer eggs. Possible applications of dry sugarcane leaf substrate for egg scouting and for trapping eggs are discussed. C1 [Showler, Allan T.] USDA ARS, IFNRRU, Kika Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Castro, Boris A.] Texas A&M AgriLife Res & Extens Ctr, Weslaco, TX 78538 USA. RP Showler, AT (reprint author), USDA ARS, IFNRRU, Kika Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. EM ashowler@weslaco.ars.usda.gov NR 44 TC 17 Z9 17 U1 1 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1180 EP 1186 DI 10.1603/EC09352 PG 7 WC Entomology SC Entomology GA 634FU UT WOS:000280566500020 PM 20857726 ER PT J AU Hoffmann, EJ Vandervoort, C Wise, JC AF Hoffmann, Eric J. Vandervoort, Christine Wise, John C. TI Plum Curculio (Coleoptera: Curculionidae) Adult Mortality and Associated Fruit Injury After Exposure to Field-Aged Insecticides on Tart Cherry Branches SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE plant-insect-chemistry triad; sublethal effects; antifeedant; oviposition deterrent ID CONOTRACHELUS-NENUPHAR; DIPTERA TEPHRITIDAE; CURATIVE ACTIVITY; EPICUTICULAR WAX; APPLE MAGGOT; FLY DIPTERA; OVIPOSITION; MANAGEMENT; CUTICLE; LETHAL AB Plum curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), adults were exposed to field-aged residues of thiamethoxam, acetamiprid, thiacloprid, indoxacarb, or azinphos-methyl on tart cherry, Prunus cerasus L. variety Montmorency. At 1, 3, 7, and 14 d postapplication, fruit were sampled for chemical residues, and bioassays were used to assess beetle mortality and plant tissue injury. Azinphos-methyl had lethal activity within 1 d of exposure at all postapplication intervals and significant fruit protection extended to 14 d postapplication. All of the neonicotinoids had lethal activity at 3 d posttreatment, with acetamiprid activity extending to 7 d. Antifeedant and oviposition deterrent effects were seen with thiamethoxam and thiacloprid; damage incidence was significantly reduced in the absence of significant beetle mortality or intoxication. Thiamethoxam and acetamiprid penetrated into leaf and fruit tissue and were detected in the interior tissues at 14 d postapplication, but interior thiacloprid residues were not detected after day 1. Indoxacarb provided some fruit protection out to 7 d postapplication, and 14-d-old residues intoxicated beetles, but the slow action of this compound allowed significant damage to occur before beetles were incapacitated. Indoxacarb was only detected as a surface residue after the first day postapplication. These data on the plant-insect-chemistry interactions will support use and management decisions as compounds with acute contact activity are phased out. C1 [Hoffmann, Eric J.] USDA, Arid Lands Agr Res Ctr, Maricopa, AZ 85238 USA. [Vandervoort, Christine] Michigan State Univ, Pesticide Analyt Lab, Ctr Integrated Plant Syst 206, E Lansing, MI 48824 USA. [Wise, John C.] Michigan State Univ, Ctr Integrated Plant Syst, E Lansing, MI 48824 USA. RP Hoffmann, EJ (reprint author), USDA, Arid Lands Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85238 USA. EM eric.hoffmann@ars.usda.gov FU USDA-CSREES [MICL08338] FX We thank Amanda Carper for maintaining our northern strain plum curculio source colony, Jason Seward for managing the orchard maintenance and pesticide applications, and Tom Guest for residue preparation assistance. Statistical assistance was provided by Wei Wang and Bruce Mackey. Funding for this project was provided, in part, by the USDA-CSREES grant MICL08338 entitled "Development and Optimization of Pre- and Postharvest Pest Management Strategies in Cherries: A Multi-Tactic Approach." NR 38 TC 4 Z9 4 U1 1 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1196 EP 1205 DI 10.1603/EC10017 PG 10 WC Entomology SC Entomology GA 634FU UT WOS:000280566500022 PM 20857728 ER PT J AU Miller, DR Millar, JG Mangini, A Crowe, CM Grant, GG AF Miller, Daniel R. Millar, Jocelyn G. Mangini, Alex Crowe, Christopher M. Grant, Gary G. TI (3Z,6Z,9Z,12Z,15Z)-Pentacosapentaene and (Z)-11-Hexadecenyl Acetate: Sex Attractant Blend for Dioryctria amatella (Lepidoptera: Pyralidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene; Dioryctria amatella; Dioryctria merkeli; Dioryctria disclusa; Dioryctria clarioralis ID PINE SEED ORCHARDS; FIR CONEWORM; ABIETIVORELLA; IDENTIFICATION; PHEROMONES; INSECTS; LURE AB In 2006-2008, we tested (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene (pentaene) with the pheromone components (Z)-11-hexadecenyl acetate (Z11-16:Ac) and (Z) -9-tetradecenyl acetate (Z9-14:Ac), as sex attractants for four sympatric species of coneworms, Dioryctria Zeller (Lepidoptera: Pyralidae) in slash (Pinus elliottii Engelm.) and loblolly pine (Pinus taeda L.) seed orchards in Georgia and Louisiana, respectively. The addition of pentaene increased catches of male southern pine coneworm, Dioryctria amatella (Hulst), in wing traps baited with Z11-16:Ac, whereas catches of Dioryctria disclusa Heinrich in traps baited with Z9-14:Ac were unaffected by the addition of pentaene. The effect of pentaene on male Dioryctria merkeli Mutuura & Munroe was inconsistent. In 2006, pentaene seemed to inhibit attraction of D. merkeli to traps baited with Z9-14:Ac, whereas in a subsequent trial in 2008, moths were equally attracted to Z9-14:Ac with or without the pentaene. We caught too few Dioryctria clarioralis (Walker) in any experiment for meaningful analyses. Our field results with pentaene and the unresolved complexity of the taxonomy, ecology, and management of southern coneworms support the need for a comprehensive examination of the chemical ecology of Dioryctria spp. C1 [Miller, Daniel R.; Crowe, Christopher M.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. [Millar, Jocelyn G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Mangini, Alex] US Forest Serv, USDA, Alexandria Field Off, Pineville, LA 71360 USA. [Grant, Gary G.] Canadian Forest Serv, Great Lakes Forestry Ctr, Marie, ON P6A 2E5, Canada. RP Miller, DR (reprint author), US Forest Serv, USDA, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM dmiller03@fs.fed.us NR 26 TC 4 Z9 5 U1 1 U2 2 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1216 EP 1221 DI 10.1603/EC09412 PG 6 WC Entomology SC Entomology GA 634FU UT WOS:000280566500024 PM 20857730 ER PT J AU Francese, JA Crook, DJ Fraser, I Lance, DR Sawyer, AJ Mastro, VC AF Francese, Joseph A. Crook, Damon J. Fraser, Ivich Lance, David R. Sawyer, Alan J. Mastro, Victor C. TI Optimization of Trap Color for Emerald Ash Borer (Coleoptera: Buprestidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE emerald ash borer; prism trap; visual cues; trap height ID AGRILUS-PLANIPENNIS COLEOPTERA; CAPTURE AB Field assays were performed to determine the optimal color for Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) traps. Previous studies have found that more A. planipennis are caught on purple or green traps than traps of other colors. In three studies, we evaluated various shades of purple, wavelengths of green (500-570 nm), and greens of different reflectance (from 9 to 66%). In all tests, traps of corrugated plastic in standard, commercially available purple (currently used to survey A. planipennis) and a customized green color were used as bases for comparison. Among purple traps, a paint color previously shown to be generally attractive to buprestids caught significantly more A. planipennis adults than traps coated with paints containing more blue or red, or traps constructed of the standard purple plastic. Among traps with maximum reflectance at varying green wavelengths, those ranging in wavelength from 525 to 540 nm caught significantly more adult A. planipennis than traps of other wavelengths. In the 530-540 nm range of the electromagnetic spectrum, there was no significant difference among traps in the 23-66% reflectance range, but traps painted with a peak reflectance of 49% caught more beetles than purple or the custom green plastic traps. Male to female ratio was highest on green traps. C1 [Francese, Joseph A.; Crook, Damon J.; Lance, David R.; Sawyer, Alan J.; Mastro, Victor C.] USDA APHIS PPQ CPHST, Otis Lab, Otis Angb, MA 02542 USA. [Fraser, Ivich] USDA APHIS PPQ, Emerald Ash Borer Project, Brighton, MI 48116 USA. RP Francese, JA (reprint author), USDA APHIS PPQ CPHST, Otis Lab, Bldg 1398, Otis Angb, MA 02542 USA. EM joe.francese@aphis.usda.gov FU USDA-APHIS-PPQ Emerald Ash Borer FX This research was funded by the USDA-APHIS-PPQ Emerald Ash Borer program. We thank the staff of the USDA-APHIS-PPQ Emerald Ash Borer laboratory (Brighton, MI) for fieldwork assistance: Michael Rietz, Nicole Smith, Janna Daimler, John DeLisle, Kyle Gies, Joshua Warlick, Kelly Donovan, Bethany Boegler, Matthew Haack, Derek Lamarand, Stephanie Likens, Ariel Andrews, Kevin Donovan, and Kelly Schmiermund. Jason Oliver and Nadeer Youssef (Tennessee State University, McMinnville, TN) provided the formula for TSU purple. Sara Grady assisted with trap painting. Miriam Cooperband, Phil Lewis (Otis Lab), and Jason Oliver reviewed an earlier version of this manuscript. NR 19 TC 35 Z9 39 U1 2 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1235 EP 1241 DI 10.1603/EC10088 PG 7 WC Entomology SC Entomology GA 634FU UT WOS:000280566500026 PM 20857732 ER PT J AU Siegel, JP Kuenen, LPSB Ledbetter, C AF Siegel, Joel P. Kuenen, L. P. S. Bas Ledbetter, Craig TI Variable Development Rate and Survival of Navel Orangeworm (Lepidoptera: Pyralidae) on Wheat Bran Diet and Almonds SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE navel orangeworm; almonds; development; survival; degree-days ID AMYELOIS-TRANSITELLA LEPIDOPTERA; SEX-PHEROMONE; WALKER LEPIDOPTERA; IDENTIFICATION; PISTACHIOS; CALIFORNIA; BEHAVIOR AB A series of laboratory and field studies were conducted using three lines of navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), reared on wheat bran diet and almonds, Prunus dulcis (Mill.) D.A.Webb, at constant and fluctuating temperature. The duration of development on wheat bran diet at constant temperature differed significantly among the three lines. Development was as much as 40% faster at constant temperature than at fluctuating temperatures, consequently the developmental duration determined at constant temperature was not an absolute measure. When the maximum temperature in fluctuating regimes exceeded 43 degrees C, survival decreased by 50% compared with the constant temperature control. In almonds held at constant temperature, the developmental rate on new-crop nuts was variety-dependent and was fastest on 'Nonpareil' almonds and slowest on the experimental selection '23-122'. Development and survival were also variety-dependent on unharvested (mummy) almonds, and navel orangeworm average emergence was earliest from Nonpareil and latest from 'Carmel' nuts, differing by 529 degree-days, whereas survival was the highest on 'Butte', 35.7%, and the lowest on Carmel nuts, 7.2%. In our trials, both the speed of development and survival depended on host age, variety and quality, indicating that almonds were a dynamic rather than a static nutrient source for navel orangeworm. Identifying the factors responsible for variation in development and survival will give insight into improving control strategies. C1 [Siegel, Joel P.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Commod Protect & Qual Unit, Parlier, CA 93648 USA. [Ledbetter, Craig] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Unit, Parlier, CA 93648 USA. EM joel.siegel@ars.usda.gov NR 33 TC 12 Z9 13 U1 0 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1250 EP 1257 DI 10.1603/EC09309 PG 8 WC Entomology SC Entomology GA 634FU UT WOS:000280566500028 PM 20857734 ER PT J AU Li, AY Davey, RB Miller, RJ AF Li, Andrew Y. Davey, Ronald B. Miller, Robert J. TI Laboratory Evaluation of Verbutin as a Synergist of Acaricides Against Larvae of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE verbutin; PBO; synergist; bioassay; acaricide resistance ID INSECTICIDE SYNERGISTS; SUSCEPTIBILITY; RESISTANCE; AMITRAZ AB Synergistic effects of verbutin, a member of aryl alkynyl derivatives, to three commonly used acaricides were evaluated with the modified Food and Agricultural Organization Larval Packet Test (FAO-LPT) against both susceptible and resistant strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae). These tick strains demonstrated various levels of resistance to coumaphos (2.5-8.2x), permethrin (57.9-711.7x), and amitraz (3.5-177.5x). Verbutin alone was more toxic to tick larvae than piperonyl butoxide (PBO), a conventional pesticide synergist. With synergism ratios (SRs) ranging from 1.5 to 6.0, verbutin was more potent than PBO (SR = 0.9-1.6) in synergizing coumaphos toxicity to resistant tick larvae. Similarly, verbutin (SR = 1.8-15) was also found to be more potent than PBO (SR = 0.9-2.5) in synergizing amitraz in resistant tick strains. Both verbutin and PBO significantly increased permethrin toxicity to larvae of all tick strains tested, and there was no significant difference between the two synergists. SRs ranged from 2.1 to 4.4 and from 2.1 to 3.6 for PBO and verbutin, respectively. C1 [Li, Andrew Y.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Davey, Ronald B.; Miller, Robert J.] ARS, USDA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. RP Li, AY (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM andrew.li@ars.usda.gov NR 22 TC 1 Z9 2 U1 0 U2 5 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1360 EP 1364 DI 10.1603/EC09329 PG 5 WC Entomology SC Entomology GA 634FU UT WOS:000280566500042 PM 20857748 ER PT J AU Akbar, W Showler, AT Reagan, TE White, WH AF Akbar, W. Showler, A. T. Reagan, T. E. White, W. H. TI Categorizing Sugarcane Cultivar Resistance to the Sugarcane Aphid and Yellow Sugarcane Aphid (Hemiptera: Aphididae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Melanaphis sacchari; Sipha flava; intrinsic rate of increase; Saccharum resistance ID GREENBUG HOMOPTERA; CHLOROPHYLL LOSS; MELANAPHIS-SACCHARI; BORER LEPIDOPTERA; FEEDING DAMAGE; WHEAT; SORGHUM; GERMPLASM; INCREASE; LEAF AB Sugarcane (Saccharum spp.) in Louisiana is colonized by two aphid species, the sugarcane aphid, Melanaphis sacchari (Zehntner), and the yellow sugarcane aphid, Sipha flava (Forbes) (Hemiptera: Aphididae). The main problem associated with M. sacchari is transmission of sugarcane yellow leaf virus, a casual agent of yellow leaf disease whose absence has been added to certification standards for micropropagated sugarcane in Louisiana. Greenhouse studies were conducted to categorize dominant commercial sugarcane cultivars for their ability to tolerate aphid injury and to express antixenotic or antibiotic effects on both aphid species. Antixenosis tests showed no preference among cultivars by either aphid species. Loss of chlorophyll content in tolerance tests also did not show differences among cultivars for both aphid species. However, antibiosis tests revealed that life history parameters such as the duration of the reproductive period and fecundity of both aphid species were negatively affected on `HoCP 91-555' compared with 'L 97-128'. Estimation of demographic statistics indicated that both aphid species exhibited a significantly lower intrinsic rate of increase (1.8-2.8-fold) and longer doubling time (1.7-3.1-fold) on HoCP 91-555 relative to L 97-128. From these tests, cultivars in the current study can be ranked from most to the least susceptible as L 97-128 > 'LCP 85-384' > `HoCP 96-540' > 'Ho 95-988' > HoCP 91-555 for M. sacchari and L 97-128 > LCP 85-384 > HoCP 91-555 for S. flava. Therefore, antibiosis is an important category of resistance in sugarcane to both aphid species, and HoCP 91-555 might provide useful germplasm for developing aphid resistant cultivars. C1 [Akbar, W.; Reagan, T. E.] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Entomol, Baton Rouge, LA 70803 USA. [Showler, A. T.] USDA ARS, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [White, W. H.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. RP Akbar, W (reprint author), Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Entomol, Baton Rouge, LA 70803 USA. EM wakbar@agcenter.lsu.edu NR 47 TC 11 Z9 12 U1 1 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1431 EP 1437 DI 10.1603/EC09336 PG 7 WC Entomology SC Entomology GA 634FU UT WOS:000280566500052 PM 20857758 ER PT J AU Weng, YQ Perumal, A Burd, JD Rudd, JC AF Weng, Yiqun Perumal, Azhaguvel Burd, John D. Rudd, Jackie C. TI Biotypic Diversity in Greenbug (Hemiptera: Aphididae): Microsatellite-Based Regional Divergence and Host-Adapted Differentiation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Schizaphis graminum; microsatellite; simple sequence repeat; biotype; genetic diversity ID RUSSIAN WHEAT APHID; DNA SEQUENCE DIVERGENCE; SCHIZAPHIS-GRAMINUM; SITOBION-AVENAE; RESISTANT SORGHUM; GENETIC-STRUCTURE; BEMISIA-TABACI; SWEET-POTATO; PEA APHID; HOMOPTERA AB Nineteen isolates of the cereal aphid pest greenbug, Schizaphis graminum (Rondani) (Hemiptera: Aphididae), were collected from wheat, Triticum aestivum L.; barley, Hardeum vulgare L.; or noncultivated grass hosts in five locations from Colorado and Wyoming. Parthenogenetic colonies were established. Biotypic profiles of the 19 isolates were determined based on their abilities to damage a set of host plant differentials, and 13 new biotypes were identified. Genetic diversity among the 19 isolates and five previously designated greenbug biotypes (E, G, H, I, and K) was examined with 31 cross-species transferable microsatellite (simple sequence repeat) markers. Neighbor-joining clustering analysis of marker data revealed host-adapted genetic divergence as well as regional differentiation of greenbug populations. Host associated biotypic variation seems to be more obvious in "agricultural biotypes," whereas isolates collected from noncultivated grasses tend to show more geographic divergence. It seems that the biotype sharing the most similar biotypic profiles and the same geographic region with current prevailing one may have the greatest potential to become the new prevailing biotype. Close monitoring of greenbug population dynamics especially biotypic variation on both crop plants and noncultivated grasses in small grain production areas may be a useful strategy for detecting potentially new prevailing virulent biotypes of the greenbug. C1 [Weng, Yiqun; Perumal, Azhaguvel; Rudd, Jackie C.] Texas AgriLife Res, Amarillo, TX 79106 USA. [Burd, John D.] USDA ARS, Plant Sci Res Lab, Stillwater, OK 74075 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM yiqun.weng@ars.usda.gov; john.burd@ars.usda.gov FU United States Department of Agriculture; National Research Initiative [CSREES 2006-35301-16892] FX We thank M. Burrows for technical assistance. This research was partially supported by the United States Department of Agriculture, National Research Initiative Competitive Grant Program grant CSREES 2006-35301-16892 (to Y.W.). NR 50 TC 13 Z9 14 U1 2 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1454 EP 1463 DI 10.1603/EC09291 PG 10 WC Entomology SC Entomology GA 634FU UT WOS:000280566500055 PM 20857761 ER PT J AU Throne, JE Arbogast, RT AF Throne, James E. Arbogast, Richard T. TI A Computer Model for Simulating Population Development of the Indianmeal Moth (Lepidoptera: Pyralidae) in Stored Corn SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Plodia interpunctella; simulation model; stored-product insect ID INTERPUNCTELLA HUBNER LEPIDOPTERA; PLODIA-INTERPUNCTELLA; SOUTH-CAROLINA; GRAIN ADVISER; PRODUCT MOTHS; WILD STRAIN; MEAL MOTH; MAIZE; MANAGEMENT; TEMPERATURE AB The Indianmeal moth, Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae), is a common pest of stored corn, Zea mays L. We developed a computer model to simulate population development of the Indianmeal moth in stored corn by using previously published data describing immature developmental times and survivorship, and adult longevity and fecundity. The model accurately simulated population development of Indianmeal moths in corn stored during fall and into winter of three separate storage seasons in South Carolina. This is the period when the Indianmeal moth is a pest in stored corn in South Carolina. The model predicted that populations would increase after winter as grain temperatures rose, but observed populations in the grain bins never increased after winter. Despite this, the model should be useful from a management perspective because the corn is being sold off or used up after winter, and the observed Indianmeal moth populations never reached damaging levels after winter. C1 [Throne, James E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Arbogast, Richard T.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Throne, JE (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.throne@ars.usda.gov NR 23 TC 5 Z9 5 U1 1 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1503 EP 1507 DI 10.1603/EC09400 PG 5 WC Entomology SC Entomology GA 634FU UT WOS:000280566500060 PM 20857766 ER PT J AU Khamis, M Subramanyam, B Flinn, PW Dogan, H Jager, A Gwirtz, JA AF Khamis, Moses Subramanyam, Bhadriraju Flinn, Paul W. Dogan, Hulya Jager, Abigail Gwirtz, Jeffrey A. TI Susceptibility of Various Life Stages of Rhyzopertha dominica (Coleoptera: Bostrichidae) to Flameless Catalytic Infrared Radiation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Rhyzopertha dominica; infrared radiation; nonchemical method; efficacy assessment ID WHEAT KERNELS; F COLEOPTERA; ROUGH RICE; INSECTS; MORTALITY; QUALITY AB In laboratory experiments, a flameless catalytic infrared emitter, fueled by propane, was used to disinfest hard red winter wheat, Triticum aestivum L., containing different life stages of the lesser grain borer, Rhyzopertha dominica (F.), an economically important insect species associated with stored wheat in Kansas. The emitter generates infrared radiation in the 3-7-mu m range. The life stages of R. dominica exposed to infrared radiation included eggs, larvae in different stages of development, pupae, and 2-wk-old adults. A noncontact infrared thermometer measured grain temperatures continuously during exposures of infested wheat to infrared radiation. The grain temperatures attained were influenced by wheat quantity; distance from the emitter; and exposure time, which in turn influenced effectiveness against various life stages of R. dominica. In general, higher grain temperatures were attained in 113.5 g of wheat as opposed to 227.0 g, and at 8.0 cm from the emitter surface rather than at 12.7 cm, and during a 60-s exposure compared with a 45-s exposure. Logistic regression indicated the probability of death of various life stages of R. dominica was temperature dependent. The log odds ratios showed old larvae were less susceptible to infrared radiation than young larvae. Approximately >= 94% mortality of all R. dominica life stages occurred when using 113.5 g of wheat, exposed for 60 s at a distance of 8.0 cm from the emitter, resulting in mean SE wheat temperatures that ranged between 107.6 +/- 1.4 and 113.5 +/- 0.5 degrees C. Our results with small grain quantities show flameless catalytic infrared technology to be a promising tool for disinfestation of stored wheat. C1 [Khamis, Moses; Subramanyam, Bhadriraju; Flinn, Paul W.; Dogan, Hulya; Jager, Abigail; Gwirtz, Jeffrey A.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Flinn, Paul W.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Khamis, M (reprint author), Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RI Flinn, Paul/B-9104-2013 FU USDA FX We thank Virgil Macaluso (Catalytic Drying Technologies LLC) for donating the bench top infrared emitter. Russell Taylor (Electronics Design Laboratory, Kansas State University, Manhattan, KS) developed the LabView program for real-time temperature data acquisition. This work was supported by a grant from USDA-CSREES NC-IPM funds. This article is contribution 10-265-J of the Kansas State University Agricultural Experiment Station. NR 33 TC 5 Z9 5 U1 2 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2010 VL 103 IS 4 BP 1508 EP 1516 DI 10.1603/EC10069 PG 9 WC Entomology SC Entomology GA 634FU UT WOS:000280566500061 PM 20857767 ER PT J AU Corcoran, MP Meydani, M Lichtenstein, AH Schaefer, EJ Dillard, A Lamon-Fava, S AF Corcoran, Michael P. Meydani, Mohsen Lichtenstein, Alice H. Schaefer, Ernst J. Dillard, Alice Lamon-Fava, Stefania TI Sex hormone modulation of proinflammatory cytokine and C-reactive protein expression in macrophages from older men and postmenopausal women SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID LOW-DENSITY-LIPOPROTEIN; TUMOR-NECROSIS-FACTOR; ADHESION MOLECULE-1 EXPRESSION; AORTIC ENDOTHELIAL-CELLS; BLOOD MONONUCLEAR-CELLS; REPLACEMENT THERAPY; HEART-DISEASE; ATHEROSCLEROSIS; TESTOSTERONE; CHOLESTEROL AB Inflammation plays a central role in the development and progression of coronary heart disease (CHD). The sex hormones estrogen and testosterone have been shown to modify the inflammatory response by influencing cytokine expression in human macrophages obtained from younger individuals. The effect of these hormones on the expression of proinflammatory markers in macrophages obtained from a CHD age-relevant population has not been studied. Human monocyte-derived macrophages (HMDMs) were obtained from healthy normolipidemic men and postmenopausal women (age 50-70 years), and cultured in autologous serum along with both physiological and supraphysiological concentrations of estrogen or testosterone. HMDMs were stimulated with oxidized low-density lipoproteins, and the expression of the cytokines tumor necrosis factor alpha (TNF-alpha or TNF), interleukin (IL) 6, and IL-1 beta (IL1B) and of the acute-phase protein C-reactive protein (CRP) was measured. Both physiological and supraphysiological concentrations of testosterone reduced the expression and secretion of TNF-alpha and reduced the expression of IL-1 beta, but did not affect the expression of IL6 or CRP. Estrogen did not modify the expression of TNF-alpha, IL6, and IL-1 beta. Estrogen caused a variable response in CRP expression that was positively associated with the plasma small dense LDL-cholesterol concentration of the donors. There were no gender differences in any of the observed effects. Our results indicate that testosterone may exert anti-inflammatory effects by reducing macrophage TNF-alpha expression, while the effects of estrogen on macrophage CRP expression may depend upon the extracellular lipid environment. Journal of Endocrinology (2010) 206, 217-224 C1 [Corcoran, Michael P.; Schaefer, Ernst J.; Lamon-Fava, Stefania] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. [Meydani, Mohsen] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Vasc Biol Lab, Boston, MA 02111 USA. [Lichtenstein, Alice H.; Dillard, Alice] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. RP Lamon-Fava, S (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Lipid Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM stefania.lamon-fava@tufts.edu FU United States Department of Agriculture [58-1950-7-707, T32 HL69772-01A1]; Unilever Health Science Scholarship FX This work was supported by the United States Department of Agriculture, under agreement No. 58-1950-7-707 and T32 HL69772-01A1 (MPC). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors, and do not necessarily reflect the view of the USDA. MPC was supported by the Unilever Health Science Scholarship. NR 47 TC 45 Z9 48 U1 0 U2 4 PU BIOSCIENTIFICA LTD PI BRISTOL PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND SN 0022-0795 EI 1479-6805 J9 J ENDOCRINOL JI J. Endocrinol. PD AUG PY 2010 VL 206 IS 2 BP 217 EP 224 DI 10.1677/JOE-10-0057 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 626BM UT WOS:000279940900007 PM 20484148 ER PT J AU King, KW Balogh, JC AF King, K. W. Balogh, J. C. TI Chlorothalonil and 2,4-D losses in surface water discharge from a managed turf watershed SO JOURNAL OF ENVIRONMENTAL MONITORING LA English DT Article ID RAINFALL CONDITIONS; PESTICIDE RUNOFF; GOLF-COURSES; HERBICIDES; QUALITY; FORMULATION; TURFGRASS; SIMULATIONS; IRRIGATION; TRANSPORT AB Managed turf sites (golf courses) are the most intensively managed landscapes in the urban environment. Yet, long-term watershed scale studies documenting the environmental transport of agrichemicals applied to these systems are rare. The objective of this study was to quantify the surface discharge losses of two commonly applied pesticides (chlorothalonil and 2,4-D) resulting from prevailing practices on a managed golf course. Inflow and outflow discharge waters on a subarea of Northland Country Club located in Duluth, MN were measured for both quantity and quality from April through November from 2003 to 2008. The median chlorothalonil outflow concentration (0.58 mu g L(-1)) was significantly greater (p < 0.05) than the inflow concentration, which was below the detection limit (0.07 mu g L(-1)). Similarly, the median outflow 2,4-D concentration (0.85 mu g L(-1)) was significantly greater (p < 0.05) than the inflow concentration (0.31 mu g L(-1)). Chlorothalonil concentrations occasionally exceeded acute toxicity levels (7.6 mu g L(-1)) reported for rainbow trout. No 2,4-D concentrations exceeded any human or aquatic species published toxicity level; however, the maximum measured 2,4-D concentration (67.1 mu g L(-1)), which rarely occurred, did approach the 70 mu g L(-1) maximum contaminant level (MCL) for that compound. Losses of both pesticides were detectable throughout the annual sampling period. Mean annual chlorothalonil loading was 10.5 g ha(-1) or 0.3% of applied, while mean annual 2,4-D loading was 4.9 g ha(-1) or 0.5% of applied. The findings of this study provide quantifiable evidence of agrichemical transport resulting from typical turfgrass management and highlight the need for implementation of best management practices to combat the offsite transport of agrichemicals used in professional turf management. C1 [King, K. W.] USDA ARS, Columbus, OH 43210 USA. [Balogh, J. C.] Spectrum Res Inc, Duluth, MN 55804 USA. RP King, KW (reprint author), USDA ARS, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM kevin.king@ars.usda.gov FU US Golf Association Green Section FX The authors would like to acknowledge the assistance of Sara Beth Scadlock, Ivy Leland, Emily Burgess, and Heather McMains in the data collection, processing, and analysis necessary to complete this manuscript. Without their dedication, perseverance, and attention to detail this study would not have been possible. The authors are also indebted to Dave Kolbry (past superintendent), Christopher Tritabaugh (current superintendent), and Jacob Ryan (current assistant superintendent) for granting us access to the course and sharing their management strategies and records. We would also like to thank the members of NCC for their insight in recognizing the importance of this type of research and their willingness to permit us to conduct this type of study on their course. We also acknowledge and thank the US Golf Association Green Section for their support and financial contributions to the study. NR 45 TC 3 Z9 3 U1 4 U2 13 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 1464-0325 J9 J ENVIRON MONITOR JI J. Environ. Monit. PD AUG PY 2010 VL 12 IS 8 BP 1601 EP 1612 DI 10.1039/c0em00030b PG 12 WC Chemistry, Analytical; Environmental Sciences SC Chemistry; Environmental Sciences & Ecology GA 644KH UT WOS:000281374400012 PM 20526481 ER PT J AU Peyer, SM Hermanson, JC Lee, CE AF Peyer, Suzanne M. Hermanson, John C. Lee, Carol Eunmi TI Developmental plasticity of shell morphology of quagga mussels from shallow and deep-water habitats of the Great Lakes SO JOURNAL OF EXPERIMENTAL BIOLOGY LA English DT Article DE biological invasions; bivalve; calcification; common-garden experiment; functional morphology; Great Lakes; moment of inertia; phenotypic plasticity; temperature ID DREISSENA-POLYMORPHA PALLAS; VITAMIN-D; MYTILUS-GALLOPROVINCIALIS; GROWTH-RATE; BUGENSIS; TEMPERATURE; BIVALVIA; SHAPE; ERIE; CALCIFICATION AB The invasive zebra mussel (Dreissena polymorpha) has quickly colonized shallow-water habitats in the North American Great Lakes since the 1980s but the quagga mussel (Dreissena bugensis) is becoming dominant in both shallow and deep-water habitats. While quagga mussel shell morphology differs between shallow and deep habitats, functional causes and consequences of such difference are unknown. We examined whether quagga mussel shell morphology could be induced by three environmental variables through developmental plasticity. We predicted that shallow-water conditions (high temperature, food quantity, water motion) would yield a morphotype typical of wild quagga mussels from shallow habitats, while deep-water conditions (low temperature, food quantity, water motion) would yield a morphotype present in deep habitats. We tested this prediction by examining shell morphology and growth rate of quagga mussels collected from shallow and deep habitats and reared under common-garden treatments that manipulated the three variables. Shell morphology was quantified using the polar moment of inertia. Of the variables tested, temperature had the greatest effect on shell morphology. Higher temperature (similar to 18-20 degrees C) yielded a morphotype typical of wild shallow mussels regardless of the levels of food quantity or water motion. In contrast, lower temperature (similar to 6-8 degrees C) yielded a morphotype approaching that of wild deep mussels. If shell morphology has functional consequences in particular habitats, a plastic response might confer quagga mussels with a greater ability than zebra mussels to colonize a wider range of habitats within the Great Lakes. C1 [Peyer, Suzanne M.; Lee, Carol Eunmi] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Hermanson, John C.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Lee, Carol Eunmi] Univ Wisconsin, Ctr Rapid Evolut, Madison, WI 53706 USA. RP Peyer, SM (reprint author), Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. EM smpeyer@wisc.edu RI Hermanson, John/K-8106-2015 OI Hermanson, John/0000-0002-3325-6665 FU Wisconsin Sea Grant Institute [R/LR-91]; Center for Limnology and Marine Sciences at the University of Wisconsin; Department of Zoology at the University of Wisconsin FX This study was supported by a grant from the Wisconsin Sea Grant Institute (R/LR-91, C. E. L.); an Anna Grant Birge Memorial Scholarship from the Center for Limnology and Marine Sciences at the University of Wisconsin (S. M. P.); and the Dr and Mrs Carl A. Bunde Award from the Department of Zoology at the University of Wisconsin (S. M. P.). Dawn Dittman, Robert O'Gorman, Theodore Strang, Terry Lewchanin, Maureen Walsh and Edward Perry of the U. S. Geological Survey graciously facilitated mussel collections. Mark Eriksson helped with programming required for data processing. Gregory Gelembiuk, Warren Porter, Janette Boughman, Marijan Posavi and Brian Metzger offered thoughtful discussions. Dave Hoffman and Duane Barnes helped with the initial setup for rearing mussels and Mike Kiergaard and Alice McCarthy provided periodic assistance with mussel maintenance. Robert and Beatrice Peyer assisted with water collection during memorable trips to Lake Michigan. NR 44 TC 17 Z9 18 U1 1 U2 19 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0022-0949 J9 J EXP BIOL JI J. Exp. Biol. PD AUG 1 PY 2010 VL 213 IS 15 BP 2602 EP 2609 DI 10.1242/jeb.042549 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 626PL UT WOS:000279978500016 PM 20639421 ER PT J AU Gong, PJ Zhang, JH Li, HX Yang, CX Zhang, CJ Zhang, XH Khurram, Z Zhang, YY Wang, TT Fei, ZJ Ye, ZB AF Gong, Pengjuan Zhang, Junhong Li, Hanxia Yang, Changxian Zhang, Chanjuan Zhang, Xiaohui Khurram, Ziaf Zhang, Yuyang Wang, Taotao Fei, Zhangjun Ye, Zhibiao TI Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Drought stress; gene expression; introgression lines; microarray; tomato ID LENGTH CDNA MICROARRAY; HIGH-SALINITY STRESSES; FALSE DISCOVERY RATE; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; TRANSGENIC TOBACCO; OXIDATIVE STRESS; LYCOPERSICON-ESCULENTUM; EXPRESSION PROFILES; SALT TOLERANCE AB To unravel the molecular mechanisms of drought responses in tomato, gene expression profiles of two drought-tolerant lines identified from a population of Solanum pennellii introgression lines, and the recurrent parent S. lycopersicum cv. M82, a drought-sensitive cultivar, were investigated under drought stress using tomato microarrays. Around 400 genes identified were responsive to drought stress only in the drought-tolerant lines. These changes in genes expression are most likely caused by the two inserted chromosome segments of S. pennellii, which possibly contain drought-tolerance quantitative trait loci (QTLs). Among these genes are a number of transcription factors and signalling proteins which could be global regulators involved in the tomato responses to drought stress. Genes involved in organism growth and development processes were also specifically regulated by drought stress, including those controlling cell wall structure, wax biosynthesis, and plant height. Moreover, key enzymes in the pathways of gluconeogenesis (fructose-bisphosphate aldolase), purine and pyrimidine nucleotide biosynthesis (adenylate kinase), tryptophan degradation (aldehyde oxidase), starch degradation (beta-amylase), methionine biosynthesis (cystathionine beta-lyase), and the removal of superoxide radicals (catalase) were also specifically affected by drought stress. These results indicated that tomato plants could adapt to water-deficit conditions through decreasing energy dissipation, increasing ATP energy provision, and reducing oxidative damage. The drought-responsive genes identified in this study could provide further information for understanding the mechanisms of drought tolerance in tomato. C1 [Gong, Pengjuan; Zhang, Junhong; Yang, Changxian; Zhang, Chanjuan; Zhang, Xiaohui; Khurram, Ziaf; Zhang, Yuyang; Ye, Zhibiao] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Li, Hanxia; Wang, Taotao] Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. RP Ye, ZB (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. EM zbye@mail.hzau.edu.cn FU Ministry of Science and Technology of China [2009CB119000]; National Science Foundation of China (NSFC) [30871712, 30921002]; National Science Foundation [DBI-0501778] FX We would like to thank Dr Amber Hotto for the critical review of this manuscript and Ms Julia Sharwood for proofreading. We are grateful to the Tomato Genetic Resource Center (TGRC) for supplying tomato seeds of 50 introgression lines, S. pennellii, and M82. This work was supported by grants from the Ministry of Science and Technology of China (973 Project, 2009CB119000) to HL, the National Science Foundation of China (NSFC Grants no. 30871712 and no. 30921002) to ZY, and the National Science Foundation (DBI-0501778) to ZF. NR 82 TC 70 Z9 78 U1 6 U2 47 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD AUG PY 2010 VL 61 IS 13 BP 3563 EP 3575 DI 10.1093/jxb/erq167 PG 13 WC Plant Sciences SC Plant Sciences GA 638OX UT WOS:000280905400008 PM 20643807 ER PT J AU Yeats, TH Howe, KJ Matas, AJ Buda, GJ Thannhauser, TW Rose, JKC AF Yeats, Trevor H. Howe, Kevin J. Matas, Antonio J. Buda, Gregory J. Thannhauser, Theodore W. Rose, Jocelyn K. C. TI Mining the surface proteome of tomato (Solanum lycopersicum) fruit for proteins associated with cuticle biogenesis SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Cuticle; cutin; lipid; proteome; tomato fruit; wax ID TRANSPIRATION BARRIER PROPERTIES; LIPID-TRANSFER PROTEINS; GENE-EXPRESSION; ABC TRANSPORTER; EPIDERMAL-CELLS; PLANT-TISSUES; ARABIDOPSIS; IDENTIFICATION; WAX; BIOSYNTHESIS AB The aerial organs of plants are covered by the cuticle, a polyester matrix of cutin and organic solvent-soluble waxes that is contiguous with the polysaccharide cell wall of the epidermis. The cuticle is an important surface barrier between a plant and its environment, providing protection against desiccation, disease, and pests. However, many aspects of the mechanisms of cuticle biosynthesis, assembly, and restructuring are entirely unknown. To identify candidate proteins with a role in cuticle biogenesis, a surface protein extract was obtained from tomato (Solanum lycopersicum) fruits by dipping in an organic solvent and the constituent proteins were identified by several complementary fractionation strategies and two mass spectrometry techniques. Of the similar to 200 proteins that were identified, a subset is potentially involved in the transport, deposition, or modification of the cuticle, such as those with predicted lipid-associated protein domains. These include several lipid-transfer proteins, GDSL-motif lipase/hydrolase family proteins, and an MD-2-related lipid recognition domain-containing protein. The epidermal-specific transcript accumulation of several of these candidates was confirmed by laser-capture microdissection and quantitative reverse transcription-PCR (qRT-PCR), together with their expression during various stages of fruit development. This indicated a complex pattern of cuticle deposition, and models for cuticle biogenesis and restructuring are discussed. C1 [Yeats, Trevor H.; Matas, Antonio J.; Buda, Gregory J.; Rose, Jocelyn K. C.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Howe, Kevin J.; Thannhauser, Theodore W.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Rose, JKC (reprint author), Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. EM jr286@cornell.edu RI Matas Arroyo, Antonio Javier/A-9325-2008 OI Matas Arroyo, Antonio Javier/0000-0003-4348-3930 FU NSF [DBI-0606595]; NIH [T32 GM008500] FX The authors gratefully acknowledge Dr Mike Scanlon for generously providing access to the PALM MicroBeam System. This work was supported by NSF Plant Genome Research Program grant (DBI-0606595) and THY was supported in part by an NIH chemistry/biology interface training grant (grant no. T32 GM008500). LC-ESI-MS/MS analysis was performed by Dr Sheng Zhang and the Cornell Proteomics and Mass Spectrometry Core Facility. NR 61 TC 45 Z9 46 U1 2 U2 22 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD AUG PY 2010 VL 61 IS 13 BP 3759 EP 3771 DI 10.1093/jxb/erq194 PG 13 WC Plant Sciences SC Plant Sciences GA 638OX UT WOS:000280905400024 PM 20571035 ER PT J AU Jun, W Kim, MS Cho, BK Millner, PD Chao, KL Chan, DE AF Jun, Won Kim, Moon S. Cho, Byoung-Kwan Millner, Patricia D. Chao, Kuanglin Chan, Diane E. TI Microbial biofilm detection on food contact surfaces by macro-scale fluorescence imaging SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Hyperspectral imaging; Fluorescence; Biofilm; Detection; Food processing surfaces ID INDUSTRY; INFECTIONS; OUTBREAK AB Hyperspectral fluorescence imaging methods were utilized to evaluate the potential detection of pathogenic bacterial biofilm formations on five types of food contact surface materials: stainless steel, high-density polyethylene (HDPE), plastic laminate (Formica), and two variations of polished granite. The main objective of this study was to determine a minimal number of spectral fluorescence bands suitable for detecting microbial biofilms on surfaces commonly used to process and handle food. Spots of biofilm growth were produced on sample surfaces by spot-inoculations of pathogenic Escherichia coli O157:H7 and Salmonella followed by room temperature storage for 3 days. Subsequently, hyperspectral fluorescence images were acquired from 421 to 700 nm using ultraviolet-A excitation. Both E. coli O157:H7 and Salmonella biofilms emitted fluorescence predominantly in the blue to green wavelengths with emission maxima at approximately 480 nm. A single-band image at 559 nm was able to detect the biofilm spots on stainless steel. On HDPE and granite, algorithms using different two-band ratios provided better separation of the biofilm spots from background areas than any single-band images did. The biofilm spots on stainless steel, HDPE, and granite could be detected with overall detection rate of 95%. On Formica, too many false positives were present to accurately determine an effective biofilm detection rate. This may have been due to the lower cell population density that was observed for the biofilm spots grown on Formica (approximately 4.3-6.4 log cfu cm(-2)) as compared to the other surfaces. These findings can be incorporated into developing portable hand-held imaging devices for sanitation inspection of food processing surfaces. Published by Elsevier Ltd. C1 [Jun, Won; Kim, Moon S.; Millner, Patricia D.; Chao, Kuanglin; Chan, Diane E.] ARS, USDA, EMFSL, Anim & Nat Resources Inst, Beltsville, MD 20705 USA. [Cho, Byoung-Kwan] Chungnam Natl Univ, Bioind Machinery Engn Dept, Taejon 305764, South Korea. RP Kim, MS (reprint author), ARS, USDA, EMFSL, Anim & Nat Resources Inst, Bldg 303 BARC E,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM moon.kim@ars.usda.gov NR 17 TC 21 Z9 21 U1 4 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD AUG PY 2010 VL 99 IS 3 BP 314 EP 322 DI 10.1016/j.jfoodeng.2010.03.005 PG 9 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 603WV UT WOS:000278239700010 ER PT J AU Oyarzabal, OA Oscar, TP Speegle, L Nyati, H AF Oyarzabal, Omar A. Oscar, Thomas P. Speegle, Leslie Nyati, Hilda TI Survival of Campylobacter jejuni and Campylobacter coli on Retail Broiler Meat Stored at-20, 4, or 12 degrees C and Development of Weibull Models for Survival SO JOURNAL OF FOOD PROTECTION LA English DT Article ID GROUND CHICKEN; RAW CHICKEN; TEMPERATURE; CARCASSES; POULTRY; STORAGE; SALMONELLA; PREVALENCE; GROWTH; SPP. AB Survival of Campylobacter jejum and Campylobacter coil isolated from broiler meat was investigated and modeled on retail breast meat. Meat portions were inoculated with C jejum or C at 6 4 to 6.8 log CFU/g followed by storage at -20 degrees C for 84 days or at 4 or 12 degrees C for 14 days Kinetic data within a species and temperature were fitted to the Weibull model When >= 70% of the residuals were in an acceptable prediction zone from -1 (fail-safe) to 0 5 (fail-dangerous) log units, the model was considered to have acceptable performance. Survival of Campylobacter was highest at 4 degrees C, lowest at 12 degrees C. and intermediate at -20 degrees C Survival of C jejunt and C coli was similar at -20 degrees C but was lower (P < 0 05) for C jejum than for C coli at 4 and 12 degrees C. The Weibull model provided acceptable predictions for four of six sets of dependent data with unacceptable performance for survival of C jejum at -20 and 12 degrees C. A difference in survival was observed between the two strains of C jejum tested Comparison of Weibull model predictions with data for C jejum archived in ComBase revealed mostly unacceptable performance, indicating that C jejum and C coli survival on raw broiler breast meat differs front published results for other strains and growth media Variation in Campylobacter survival among replicate storage trials was high, indicating that performance of the models can be improved by collection of additional data to better define the survival response during storage at temperatures from -20 to 12 degrees C C1 [Oyarzabal, Omar A.] Alabama State Univ, Dept Biol Sci, Montgomery, AL 36101 USA. [Oscar, Thomas P.] Univ Maryland Eastern Shore, Microbial Food Safety Res Unit, USDA, ARS, Princess Anne, MD 21853 USA. [Speegle, Leslie] Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA. [Nyati, Hilda] Natl Univ Sci & Technol, Dept Appl Biol & Biochem, Bulawayo, Zimbabwe. RP Oyarzabal, OA (reprint author), Alabama State Univ, Dept Biol Sci, 313 Life Sci Bldg,1627 Hall St, Montgomery, AL 36101 USA. OI Oyarzabal, Omar/0000-0002-7863-2767 FU Council for International Exchange of Scholars FX We thank the Fulbright Visiting Scholars Program of the Council for International Exchange of Scholars for the award provided to Hilda Nyati to perform research at the Department of Poultry Science, Auburn University We also thank Robert S Miller for his technical assistance NR 41 TC 10 Z9 10 U1 0 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2010 VL 73 IS 8 BP 1438 EP 1446 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 641XV UT WOS:000281166900005 PM 20819353 ER PT J AU Mead, G Lammerding, AM Cox, N Doyle, MP Humbert, F Kulikovskiy, A Panin, A do Nascimento, VP Wierup, M AF Mead, Geoffrey Lammerding, Anna M. Cox, Nelson Doyle, Michael P. Humbert, Florence Kulikovskiy, Alexander Panin, Alexander do Nascimento, Vladimir Pinheiro Wierup, Martin CA Salmonella Raw Poultry Writing Com TI Scientific and Technical Factors Affecting the Setting of Salmonella Criteria for Raw Poultry: Global Perspective SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ENTERICA SEROVAR ENTERITIDIS; COMPETITIVE-EXCLUSION TREATMENT; MICROBIAL RISK-ASSESSMENT; FOOD SAFETY OBJECTIVES; BUFFERED PEPTONE WATER; BROILER CARCASSES; SAMPLING METHODS; SEMISOLID RAPPAPORT; CHICKEN CARCASSES; REDUCE SALMONELLA AB Concerns about foodborne salmonellosis have led many countries to introduce microbiological criteria for certain food products. If such criteria are not well-grounded in science, they could be an unjustified obstacle to trade Raw poultry products are an Important part of the global food market Import and export ambiguities and regulatory confusion resulting from different Salmonella requirements were the impetus for convening an international group of scientific experts from 16 countries to discuss the scientific and technical Issues that affect the setting of a microbiological criterion for Salmonella contamination of raw chicken A particular concern for the group was the use of criteria implying a zero tolerance for Salmonella and suggesting complete absence of the pathogen. The notion can be interpreted differently by various stakeholders and was considered inappropriate because there is neither an effective means of eliminating Salmonella from raw poultry nor any practical method for verifying its absence Therefore, it may be more useful at present to set food safety metrics that involve reductions in hazard levels Such terms as "zero tolerance" or "absence of a microbe" in relation to raw poultry should be avoided unless defined and explained by international agreement Risk assessment provides a more meaningful approach than a zero tolerance philosophy, and new metrics, such as performance objectives that are linked to human health outcomes, should be utilized throughout the food chain to help define risk and identify ways to reduce adverse effects on public health C1 [Doyle, Michael P.] Univ Georgia, Ctr Food Safety, Griffin, GA USA. [Lammerding, Anna M.] Publ Hlth Agcy Canada, Guelph, ON, Canada. [Cox, Nelson] ARS, USDA, Athens, GA USA. [Humbert, Florence] Flowbio Veto, Ploufragan, France. [Kulikovskiy, Alexander] Minist Agr, Moscow, Russia. [do Nascimento, Vladimir Pinheiro] Univ Fed Rio Grande do Sul, Porto Alegre, RS, Brazil. [Wierup, Martin] Swedish Univ Agr Sci, Uppsala, Sweden. RP Doyle, MP (reprint author), Univ Georgia, Ctr Food Safety, Griffin, GA USA. RI Nascimento, Vladimir/G-5593-2012; Andreatti Filho, Raphael/P-3730-2015; Zwietering, Marcel/C-2479-2008 OI Zwietering, Marcel/0000-0003-2704-5690 FU Associacao Brasileira dos Produtores e Exportadores de Frangos (Brazilian Chicken Producers and Exporters Association); Asociacion de Productores Avicolas (Chilean Poultry Producers Association); Federacion de Avicultores de Honduras (Federation of Honduran Poultry Producers); Illinois Soybean Program Operation Board. the International Poultry Council; United Soybean Board, Associacion de Productores Av.; U S Poultry & Egg Export Council; USDA Foreign Agricultural Service; U S Poultry and Egg Association FX The following were invited to review the document before submission for publication and provided comments Eric Gander (Goldsboro Milling Co, Goldsboro, NC). Frank T Jones (University of Arkansas, Fayetteville, AR), Isabel Walls (USDA, Washington, DC). John Sofos (Colorado State University, Fort Collins). Carol Bates (Poultry Veterinary Services, Auckland. New Zealand). Scott Hurd (Iowa State University, Ames), and Stan Bailey (bioMerieux. Athens. GA) The authors are indebted to Dr Marcos Sanchez-Plata (Food Safety Specialist. Inter-American Program for the Promotion of Trade. Agribusiness and Food Safety, Inter-American Institute for Cooperation on Agriculture, Miami. FL) for providing information on poultry production and processing in Mexico We thank the following for financial support of this project Associacao Brasileira dos Produtores e Exportadores de Frangos (Brazilian Chicken Producers and Exporters Association), Asociacion de Productores Avicolas (Chilean Poultry Producers Association), Federacion de Avicultores de Honduras (Federation of Honduran Poultry Producers), the Illinois Soybean Program Operation Board. the International Poultry Council, the United Soybean Board, Associacion de Productores Av. the U S Poultry & Egg Export Council, the USDA Foreign Agricultural Service, and the U S Poultry and Egg Association NR 189 TC 34 Z9 36 U1 2 U2 17 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2010 VL 73 IS 8 BP 1566 EP 1590 PG 25 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 641XV UT WOS:000281166900025 PM 20819373 ER PT J AU Bett-Garber, KL Watson, MA Lea, JM Champagne, ET Lamikanra, O AF Bett-Garber, Karen L. Watson, Michael A. Lea, Jeanne M. Champagne, Elaine T. Lamikanra, Olusola TI EFFECT OF COMBINED UNDERWATER PROCESSING AND MILD PRECUT HEAT TREATMENT ON THE SENSORY QUALITY AND STORAGE OF FRESH-CUT CANTALOUPE MELON SO JOURNAL OF FOOD QUALITY LA English DT Article ID HOT-WATER; SALMONELLA; CALCIUM AB Improvement of storage quality of fresh-cut cantaloupe using a combination precut heat treatment and a modified underwater cutting treatment was determined. Eating quality was evaluated using descriptive sensory analysis, and fruit integrity was measured with respiration, cell leakage and product weight loss. Treatments included (1) control (no treatment); (2) making the first longitudinal cut underwater; (3) mild precut heat treatment in a water bath at 60C for 60 min; and (4) combination of precut heat treatment and the underwater cutting methods. Precut heating and processing underwater resulted in more intense fruity/melon flavor compared to conventional processed fresh-cut fruit. Reduced electrolyte leakage and enhanced membrane integrity were observed in all three experimental treatments, as evidenced by lower conductivity measurements. The underwater cut and combined treatments significantly reduced respiration during fresh-cut storage, reflecting less physical stress and membrane damage. Weight loss was not significantly affected by any treatment during fresh-cut storage. PRACTICAL APPLICATIONS There is a steady increase in the consumption of fresh-cut produce. To enhance the storage quality of fresh-cut cantaloupe melon, two minimal processing techniques were examined separately and combined. The methods are mild heat treatment of the whole melon at 60C for 60 min then cooling to 4C for 24 h, cutting the cantaloupe in half and removing the seeds while submerged in a calcium chloride and water solution, and the combination of the two treatments. These methods are simple and can be utilized by small or large processors to maintain sensory quality and fruit integrity during storage. C1 [Bett-Garber, Karen L.; Watson, Michael A.; Lea, Jeanne M.; Champagne, Elaine T.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA. [Lamikanra, Olusola] Frito Lay, Plano, TX USA. RP Bett-Garber, KL (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70179 USA. EM karen.bett@ars.usda.gov OI Bett-Garber, Karen/0000-0002-1453-2759 NR 20 TC 2 Z9 2 U1 0 U2 12 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0146-9428 J9 J FOOD QUALITY JI J. Food Qual. PD AUG PY 2010 VL 33 IS 4 BP 424 EP 438 DI 10.1111/j.1745-4557.2010.00337.x PG 15 WC Food Science & Technology SC Food Science & Technology GA 635QQ UT WOS:000280671600002 ER PT J AU Gurtler, JB Rivera, RB Zhang, HQ Sommers, CH AF Gurtler, Joshua B. Rivera, Rebecca B. Zhang, Howard Q. Sommers, Christopher H. TI BEHAVIOR OF AVIRULENT YERSINIA PESTIS IN LIQUID WHOLE EGG AS AFFECTED BY STORAGE TEMPERATURE, ANTIMICROBIALS AND THERMAL PASTEURIZATION SO JOURNAL OF FOOD SAFETY LA English DT Article ID ESCHERICHIA-COLI O157-H7; ANTIBACTERIAL ACTIVITY; SALMONELLA-ENTERITIDIS; LACTOPEROXIDASE SYSTEM; STAPHYLOCOCCUS-AUREUS; UNITED-STATES; CHITOSAN; PRODUCTS; ENTEROCOLITICA; INACTIVATION AB Yersinia spp. are psychrotrophic bacteria capable of growth at temperatures as low as -2C, known to contaminate shell eggs and liquid eggs in the U.S.A. and South America. A study was performed to determine the thermal sensitivity of avirulent Yersinia pestis in liquid whole egg (LWE), evaluate the growth pattern of the bacterium in LWE at temperatures of 4-22C and assess the ability of 10 antimicrobial compounds to inhibit the growth of attenuated Y. pestis in LWE. The estimated decimal reduction values of avirulent Y. pestis in LWE at 54C (D(54)) were 1.39-1.58 min, and D(60) values were 13.8 and 11.4 s by the addition of 0 and 965 IU of nisin (MP Biomedicals, LLC, Solon, OH), respectively. Low molecular weight chitosan (0.5%) and an activated lactoperoxidase system (2.18 U/mL) were ineffective at inhibiting growth of Y. pestis, while 500 IU/mL of nisin inhibited populations by up to 1 log cfu/mL at 4, 10 and 15C when compared with the control. Allyl isothiocyanate, diacetyl, diethyl dicarbonate, ethylenediaminetetraacetic acid, methylparaben, monolaurin and benzoyl peroxide inhibited the growth of attenuated Y. pestis when added at high levels. PRACTICAL APPLICATIONS The genus Yersinia does not currently pose a problem in pasteurized liquid egg products, although it has been isolated from eggs in the U.S.A. and Argentina. Yersiniae, which are psychrotrophic bacteria, can grow at temperatures as low as -2C; therefore, incidental or intentional contamination of liquid whole egg (LWE) with Yersinia spp. could result in multiplication to high populations, even when stored under refrigeration (ca. 4C). We have shown that avirulent Yersinia pestis is able to multiply to populations of > 2, 5 and 8 log cfu/mL in LWE at 4C within 6, 14 and 26 days, respectively. This study provides information that will be helpful in determining thermal and nonthermal means of controlling yersiniae in LWE products. C1 [Gurtler, Joshua B.; Rivera, Rebecca B.; Zhang, Howard Q.; Sommers, Christopher H.] ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Gurtler, JB (reprint author), ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM joshua.gurtler@ars.usda.gov NR 34 TC 8 Z9 8 U1 1 U2 6 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0149-6085 J9 J FOOD SAFETY JI J. Food Saf. PD AUG PY 2010 VL 30 IS 3 BP 537 EP 557 DI 10.1111/j.1745-4565.2010.00224.x PG 21 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 635OI UT WOS:000280664600003 ER PT J AU Sommers, CH Scullen, OJ Sites, JE AF Sommers, Christopher H. Scullen, O. Joseph Sites, Joseph E. TI INACTIVATION OF FOODBORNE PATHOGENS ON FRANKFURTERS USING ULTRAVIOLET LIGHT AND GRAS ANTIMICROBIALS SO JOURNAL OF FOOD SAFETY LA English DT Article ID CONTAIN POTASSIUM LACTATE; LISTERIA-MONOCYTOGENES; SODIUM DIACETATE; ESCHERICHIA-COLI; UV-IRRADIATION; FRESH PRODUCE; APPLE CIDER; RADIATION; INHIBITION; SURFACE AB Listeria monocytogenes is an occasional contaminant of ready-to-eat meats such as frankfurters and sausages and is responsible for foodborne illness outbreaks and recalls of the subsequently adulterated food products. Salmonella and Staphylococus aureus are prevalent among pathogens which cause foodborne illness. Ultraviolet light (UV-C) (254 nm) is a U.S. Food and Drug Administration (FDA)-approved intervention technology that can inactivate foodborne pathogens on frankfurter and precooked sausage surfaces. Potassium lactate, sodium diacetate and lauric arginate ester are U.S. FDA-approved antimicrobials that can inactivate and inhibit the growth of foodborne pathogens during refrigerated storage. UV-C light, when used in combination with the three antimicrobials, inactivated 2.32-2.80 log of the pathogens, which was more effective than either process used individually as determined by analysis of variance (n = 6, P < 0.05). During 12 weeks of refrigerated storage (10C), the use of UV-C in combination with the 3 antimicrobials was found to be very effective, with 3.6-4.1 log of the three pathogens being inactivated by the end of the storage period. UV-C and antimicrobials had no impact on frankfurter color or texture. The combinatorial use of UV-C and antimicrobials was found to be an effective hurdle against pathogen survival and proliferation. PRACTICAL APPLICATIONS Ultraviolet light (254 nm) is a U.S. Food and Drug Administration-approved technology that can be used in combination with generally recognized as safe (GRAS) antimicrobials to decontaminate the surfaces of precooked sausages such as frankfurters and bratwurst. This work indicates that ultraviolet light (UV-C), when used in combination with the commercially used antimicrobial compounds potassium lactate, sodium diacetate and lauric arginate ester, effectively inactivates and controls the growth of human pathogens better than UV-C or GRAS antimicrobials when used individually. The process described in this study can realistically be adopted by both small and large meat processors to provide safer precooked sausages for consumers. C1 [Sommers, Christopher H.; Scullen, O. Joseph; Sites, Joseph E.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Sommers, CH (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM christopher.sommers@ars.usda.gov NR 38 TC 5 Z9 5 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0149-6085 EI 1745-4565 J9 J FOOD SAFETY JI J. Food Saf. PD AUG PY 2010 VL 30 IS 3 BP 666 EP 678 DI 10.1111/j.1745-4565.2010.00232.x PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 635OI UT WOS:000280664600011 ER PT J AU Englen, MD Berrang, ME Meinersmann, RJ Fedorka-Cray, PJ AF Englen, Mark D. Berrang, Mark E. Meinersmann, Richard J. Fedorka-Cray, Paula J. TI EVALUATION OF TWO COMMERCIAL REAL-TIME PCR ASSAYS FOR DETECTING CAMPYLOBACTER IN BROILER CARCASS RINSES* SO JOURNAL OF FOOD SAFETY LA English DT Article ID JEJUNI; QUANTIFICATION; SPP. AB Traditional plating methods are reliable means for Campylobacter identification from poultry samples but automated gene-based detection systems now available can reduce assay time, data collection and analysis. Bio-Rad and DuPont Qualicon recently introduced Campylobacter assays for their real-time polymerase chain reaction (PCR) instruments. We evaluated the utility of these assays compared with standard plating and enumeration methods routinely used in our laboratory. Two replicates of 40 broiler carcass rinses collected before and after defeathering at a commercial processing plant were tested. All samples were positive for Campylobacter by direct plating of rinses: log(10) cfu values ranged from 0.24 to 4.61. In contrast, the Bio-Rad iQ-Check assay returned 60-72.5% positives on direct rinses; the Qualicon BAX Q7 test produced 60-85% positives with direct rinse samples. Using aliquots of 24 h enrichment broth from rinses in the real-time assays significantly improved detection: the Bio-Rad test had 95-100% positive while the Qualicon assay found 90-95% positive. PRACTICAL APPLICATIONS Traditional detection methods for Campylobacter are time-consuming, requiring as much as 5 days to complete. The recent introduction of commercial real-time PCR instruments for the identification of Campylobacter offer more rapid, simplified alternatives to standard culture-based techniques. C1 [Englen, Mark D.; Berrang, Mark E.; Meinersmann, Richard J.; Fedorka-Cray, Paula J.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Athens, GA 30605 USA. RP Englen, MD (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Athens, GA 30605 USA. EM mark.englen@ars.usda.gov NR 12 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0149-6085 J9 J FOOD SAFETY JI J. Food Saf. PD AUG PY 2010 VL 30 IS 3 BP 732 EP 739 DI 10.1111/j.1745-4565.2010.00237.x PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 635OI UT WOS:000280664600016 ER PT J AU Sheen, S Costa, S Cooke, P AF Sheen, Shiowshuh Costa, Sonya Cooke, Peter TI Impact of Mechanical Shear on the Survival of Listeria monocytogenes on Surfaces SO JOURNAL OF FOOD SCIENCE LA English DT Article DE L. monocytogenes; mechanical shear; surface; survival ID CROSS-CONTAMINATION; STAINLESS-STEEL; ATTACHMENT; VIABILITY; ADHESION; TEMPERATURE; DETACHMENT; BOLOGNA; STRESS; CHEESE AB The impact of mechanical surface shear on microbial viability is rarely a subject for exploration in food processing. The objective of this research was to investigate the impact of mechanical shear on the survival of Listeria mottocytogettes on surfaces. Mechanical shear created by slicing a model food was explored to investigate the viability of L. monocytogenes. Cell injury/death was readily demonstrated in fluorescence images by confocal microscopy in which the live and dead cells were fluorescently stained green and red, respectively, with a viability dye kit. Images showed that a large percentage of dead cells appeared after slicing, and they were readily transferred from the slicer blade onto the surfaces of sliced agar, indicating that surface shear may cause the lethal effect on L. monocytogenes. Surface transfer results also showed that viable cell counts on agar slices (in a slicing series) followed a consistently decreasing pattern. The cell counts initially at 5 to 6.5 log CFU/slice (slices 1 to 6), decreased to 3 to 4 log CFU/slice (slices 8 to 30), then to 2 to 3 log CFU/slice (slices 31 to 40), and counts would be expected to further decrease if slicing continued. The overall cell recovery (survival) ratio was about 2% to 3% compared to the initial 8.4 log CFU/blade on a 10 cm(2) edge area. The impact of shear on microbial viability during slicing may contribute 99% of viable cell count reduction. This study provides clear evidence that surface shear can kill foodborne pathogens and reduce cross-contamination. The lethal effects of surface shear may further enhance food safety C1 [Sheen, Shiowshuh; Costa, Sonya; Cooke, Peter] ARS, Eastern Reg Res Ctr, Microbial Food Safety Res Unit, USDA, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), ARS, Eastern Reg Res Ctr, Microbial Food Safety Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov NR 23 TC 2 Z9 2 U1 0 U2 7 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2010 VL 75 IS 6 BP E387 EP E393 DI 10.1111/j.1750-3841.2010.01692.x PG 7 WC Food Science & Technology SC Food Science & Technology GA 644JN UT WOS:000281372000030 PM 20722924 ER PT J AU Liao, CH Cooke, PH Niemira, BA AF Liao, C. -H. Cooke, P. H. Niemira, B. A. TI Localization, Growth, and Inactivation of Salmonella Saintpaul on Jalapeno Peppers SO JOURNAL OF FOOD SCIENCE LA English DT Article DE growth; inactivation; jalapeno peppers; localization; Salmonella Saintpaul ID PSEUDOMONAS-FLUORESCENS; FRESH PRODUCE; UNITED-STATES; MONTEVIDEO; SANITIZER; TOMATOES; ALFALFA; CHESTER AB Consumption of Salmonella-contaminated jalapeno peppers has been implicated in one of the largest foodborne illness outbreaks in the summer of 2008. The objective of this study was to investigate representative groups of native microflora and the distribution, growth, and inactivation of experimentally-inoculated Salmonella Saintpaul on jalapeno peppers. Two genetically modified strains of Saint. Saintpaul producing either green- or red-fluorescent protein were constructed and used in the study. Microbiological analyses showed that jalapeno peppers contained an average of 5.6 log units of total aerobic count and 3.5, 1.8, and 1.9 log units, respectively, of enterobacteriaceae, lactic acid bacteria, and yeast/mold per gram of tissue. Strains typical of Pseudomonas accounted for 8.3% of total aerobic count, and 0.2% of which exhibited pectolytic activity. On inoculated peppers, a vast majority (>90%) of Salm. Saintpaul was recovered from stem/calyx and only a small proportion recovered from fleshy pods. Growth of Saint. Saintpaul on peppers was indicated by an increase in the population of 3 log units after incubation of samples at 20 degrees C for 48 h. Fluorescent Salm. Saintpaul aggregates could be readily detected on stem/calyx using stereofluorescence imaging microscopy and scanning electron microscopy. Data presented showed that Salm. Saintpaul could survive for at least 8 wk on peppers stored at 4 degrees C. Immersion of inoculated peppers in 200 ppm of sodium hypochlorite, acidified sodium chlorite, or peroxy acetic acid for 10 min could reduce the number of Saint. Saintpaul on stem/calyx by 1.5 to 1.7 and that on flesh by 2.1 to 2.4 log units. C1 [Liao, C. -H.; Cooke, P. H.; Niemira, B. A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Niemira, BA (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Brendan.Niemira@ars.usda.gov NR 21 TC 13 Z9 13 U1 0 U2 5 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2010 VL 75 IS 6 BP M377 EP M382 DI 10.1111/j.1750-3841.2010.01667.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 644JN UT WOS:000281372000038 PM 20722940 ER PT J AU Lim, HS Vaira, AM Bae, H Bragg, JN Ruzin, SE Bauchan, GR Dienelt, MM Owens, RA Hammond, J AF Lim, Hyoun-Sub Vaira, Anna Maria Bae, Hanhong Bragg, Jennifer N. Ruzin, Steven E. Bauchan, Gary R. Dienelt, Margaret M. Owens, Robert A. Hammond, John TI Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID GENE BLOCK PROTEINS; FLUORESCENT PROTEIN; HYDROPHOBIC SEGMENT; SYSTEMIC MOVEMENT; ENCODED PROTEINS; IN-VITRO; X TGBP3; RNA; POTEXVIRUS; BARLEY AB Cell-to-cell movement of potexviruses requires coordinated action of the coat protein and triple gene block (TGB) proteins. The structural properties of Alternanthera mosaic virus (AltMV) TGB3 were examined by methods differentiating between signal peptides and transmembrane domains, and its subcellular localization was studied by Agrobacterium-mediated transient expression and confocal microscopy. Unlike potato virus X (PVX) TGB3, AltMV TGB3 was not associated with the endoplasmic reticulum, and accumulated preferentially in mesophyll cells. Deletion and site-specific mutagenesis revealed an internal signal VL(17,18) of TGB3 essential for chloroplast localization, and either deletion of the TGB3 start codon or alteration of the chloroplast-localization signal limited cell-to-cell movement to the epidermis, yielding a virus that was unable to move into the mesophyll layer. Overexpression of AltMV TGB3 from either AltMV or PVX infectious clones resulted in veinal necrosis and vesiculation at the chloroplast membrane, a cytopathology not observed in wild-type infections. The distinctive mesophyll and chloroplast localization of AltMV TGB3 highlights the critical role played by mesophyll targeting in virus long-distance movement within plants. C1 [Lim, Hyoun-Sub; Vaira, Anna Maria; Dienelt, Margaret M.; Owens, Robert A.; Hammond, John] ARS, USDA, Inst Plant Sci, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Vaira, Anna Maria] CNR, Ist Virol Vegetale, I-10135 Turin, Italy. [Bae, Hanhong] Yeungnam Univ, Sch Biotechnol, Geongsan 712749, South Korea. [Bragg, Jennifer N.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Ruzin, Steven E.] Univ Calif Berkeley, CNR, Berkeley, CA 94720 USA. [Bauchan, Gary R.] ARS, USDA, Inst Plant Sci, Elect & Confocal Microscopy Unit, Beltsville, MD 20705 USA. RP Hammond, J (reprint author), ARS, USDA, Inst Plant Sci, Mol Plant Pathol Lab, B-004,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM John.Hammond@ars.usda.gov RI Vaira, Anna Maria/B-9082-2015 NR 54 TC 15 Z9 15 U1 0 U2 7 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD AUG PY 2010 VL 91 BP 2102 EP 2115 DI 10.1099/vir.0.019448-0 PN 8 PG 14 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 638WK UT WOS:000280928500026 PM 20392901 ER PT J AU Dutra, E Balsamo, G Viterbo, P Miranda, PMA Beljaars, A Schar, C Elder, K AF Dutra, Emanuel Balsamo, Gianpaolo Viterbo, Pedro Miranda, Pedro M. A. Beljaars, Anton Schaer, Christoph Elder, Kelly TI An Improved Snow Scheme for the ECMWF Land Surface Model: Description and Offline Validation SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID TERRESTRIAL WATER STORAGE; INTERACTIVE MULTISENSOR SNOW; ICE MAPPING SYSTEM; INTERCOMPARISON PROJECT; NORTHERN-HEMISPHERE; HYDROLOGICAL CYCLE; ERA-40 REANALYSIS; SUMMER MONSOON; CLIMATE MODEL; HEAT-BALANCE AB A new snow scheme for the European Centre for Medium-Range Weather Forecasts (ECMWF) land surface model has been tested and validated. The scheme includes a new parameterization of snow density, incorporating a liquid water reservoir, and revised formulations for the subgrid snow cover fraction and snow albedo. Offline validation (covering a wide range of spatial and temporal scales) includes simulations for several observation sites from the Snow Models Intercomparison Project-2 (SnowMIP2) and global simulations driven by the meteorological forcing from the Global Soil Wetness Project-2 (GSWP2) and by ECMWF Re-Analysis ERA-Interim. The new scheme reduces the end of season ablation biases from 10 to 2 days in open areas and from 21 to 13 days in forest areas. Global GSWP2 results are compared against basin-scale runoff and terrestrial water storage. The new snow density parameterization increases the snow thermal insulation, reducing soil freezing and leading to an improved hydrological cycle. Simulated snow cover fraction is compared against NOAA/National Environmental Satellite, Data, and Information Service (NESDIS) with a reduction of the negative bias of snow-covered area of the original snow scheme. The original snow scheme had a systematic negative bias in surface albedo when compared against Moderate Resolution Imaging Spectroradiometer (MODIS) remote sensing data. The new scheme reduces the albedo bias, consequently reducing the spatial- and time-averaged surface net shortwave radiation bias by 5.2 W m 22 in 14% of the Northern Hemisphere land. The new snow scheme described in this paper was introduced in the ECMWF operational forecast system in September 2009 (cycle 35R3). C1 [Dutra, Emanuel] Univ Lisbon, CGUL, IDL, Fac Ciencias, P-1749016 Lisbon, Portugal. [Dutra, Emanuel; Schaer, Christoph] ETH, Inst Atmospher & Climate Sci, Zurich, Switzerland. [Balsamo, Gianpaolo; Beljaars, Anton] European Ctr Medium Range Weather Forecasts, Reading RG2 9AX, Berks, England. [Viterbo, Pedro] Inst Meteorol, Lisbon, Portugal. [Elder, Kelly] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO USA. RP Dutra, E (reprint author), Univ Lisbon, CGUL, IDL, Fac Ciencias, Ed C8, P-1749016 Lisbon, Portugal. EM endutra@gmail.com RI Miranda, Pedro/B-6971-2008; Balsamo, Gianpaolo/I-3362-2013; Schar, Christoph/A-1033-2008; Dutra, Emanuel/A-3774-2010; Viterbo, Pedro/B-7184-2008 OI Miranda, Pedro/0000-0002-4288-9456; Balsamo, Gianpaolo/0000-0002-1745-3634; Schar, Christoph/0000-0002-4171-1613; Dutra, Emanuel/0000-0002-0643-2643; Viterbo, Pedro/0000-0001-6587-3062 FU Portuguese Foundation for Science and Technology (FCT) [AMIC PTDC/AAC-CLI/109030/2008]; European Union; FCT [SFRH/BD/35789/2007]; Fundacao Calouste Gulbenkian FX The authors thank SnowMIP2 data providers: David Gustafsson and Manfred Stahliand (Alptal-Switzerland); Alan Barr and Paul Bartlett (Berms-Canada); Kazuyoshi Suzuki and Tomoyoshi Hirota (Hitsujigaoka-Japan); Nuria Altimir and Timo Vesala (Hyytiala-Finland); and Richard Essery and Nick Rutter for the project organization and useful comments. The BSWB dataset was kindly provided by Martin Hirschi. We also thank the MODIS Land and Albedo teams and in particular Crystal Schaaf for the valuable support and comments regarding the use of MODIS albedo product. The comments of three anonymous reviewers are gratefully acknowledged. This work was supported by the Portuguese Foundation for Science and Technology (FCT) under project AMIC PTDC/AAC-CLI/109030/2008 cofinanced by the European Union under program FEDER. E. Dutra acknowledges the financial support of FCT under Grant SFRH/BD/35789/2007 and Fundacao Calouste Gulbenkian. NR 63 TC 80 Z9 80 U1 0 U2 23 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X J9 J HYDROMETEOROL JI J. Hydrometeorol. PD AUG PY 2010 VL 11 IS 4 BP 899 EP 916 DI 10.1175/2010JHM1249.1 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 639EY UT WOS:000280955600004 ER PT J AU Walton, SL Bischoff, KM van Heiningen, ARP van Walsum, GP AF Walton, Sara L. Bischoff, Kenneth M. van Heiningen, Adriaan R. P. van Walsum, G. Peter TI Production of lactic acid from hemicellulose extracts by Bacillus coagulans MXL-9 SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Hemicellulose; Extraction; Bacillus coagulans; Fermentation; Lactic acid ID DETOXIFICATION; FERMENTATION; BIOCONVERSION; OPTIMIZATION; HARDWOODS; ETHANOL AB Bacillus coagulans MXL-9 was found capable of growing on pre-pulping hemicellulose extracts, utilizing all of the principle monosugars found in woody biomass. This organism is a moderate thermophile isolated from compost for its pentose-utilizing capabilities. It was found to have high tolerance for inhibitors such as acetic acid and sodium, which are present in pre-pulping hemicellulose extracts. Fermentation of 20 g/l xylose in the presence of 30 g/l acetic acid required a longer lag phase but overall lactic acid yield was not diminished. Similarly, fermentation of xylose in the presence of 20 g/l sodium increased the lag time but did not affect overall product yield, though 30 g/l sodium proved completely inhibitory. Fermentation of hot water-extracted Siberian larch containing 45 g/l total monosaccharides, mainly galactose and arabinose, produced 33 g/l lactic acid in 60 h and completely consumed all sugars. Small amounts of co-products were formed, including acetic acid, formic acid, and ethanol. Hemicellulose extract formed during autohydrolysis of mixed hardwoods contained mainly xylose and was converted into lactic acid with a 94% yield. Green liquor-extracted hardwood hemicellulose containing 10 g/l acetic acid and 6 g/l sodium was also completely converted into lactic acid at a 72% yield. The Bacillus coagulans MXL-9 strain was found to be well suited to production of lactic acid from lignocellulosic biomass due to its compatibility with conditions favorable to industrial enzymes and its ability to withstand inhibitors while rapidly consuming all pentose and hexose sugars of interest at high product yields. C1 [Walton, Sara L.; van Heiningen, Adriaan R. P.; van Walsum, G. Peter] Univ Maine, Dept Chem Engn, Orono, ME 04469 USA. [Bischoff, Kenneth M.] USDA, Agr Res Stn, Peoria, IL 61604 USA. RP van Walsum, GP (reprint author), Univ Maine, Dept Chem Engn, 5737 Jenness Hall, Orono, ME 04469 USA. EM PvanWalsum@umche.maine.edu FU National Science Foundation [0554545]; Maine Technology Institute; University of Maine Graduate School FX The authors gratefully acknowledge that this material is based upon work supported by the National Science Foundation under Grant No. 0554545. The authors also wish to thank Ken Bischoff of the USDA ARS for providing Bacillus coagulans MXL-9. Additional funding was provided by a Maine Technology Institute grant and a fellowship to the author from the University of Maine Graduate School. NR 28 TC 33 Z9 34 U1 4 U2 23 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD AUG PY 2010 VL 37 IS 8 BP 823 EP 830 DI 10.1007/s10295-010-0727-4 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 637UW UT WOS:000280845200007 PM 20454831 ER PT J AU Kutschbach-Brohl, L Washburn, BE Bernhardt, GE Chipman, RB Francoeur, LC AF Kutschbach-Brohl, Lisa Washburn, Brian E. Bernhardt, Glen E. Chipman, Richard B. Francoeur, Laura C. TI Arthropods of a semi-natural grassland in an urban environment: the John F. Kennedy International Airport, New York SO JOURNAL OF INSECT CONSERVATION LA English DT Article DE John F. Kennedy International Airport; Insects; Grassland; Sweep sample; Vacuum sample; Urban entomology ID MANAGEMENT-PRACTICES; BUTTERFLY DIVERSITY; SEASONAL ABUNDANCE; INSECT COMMUNITIES; TALLGRASS PRAIRIES; CAPTURE EFFICIENCY; SPECIES RICHNESS; SUCTION SAMPLER; GOLF-COURSES; LAND-USE AB Semi-natural grassland habitat fragments, such as those found on airports, might be important for arthropod conservation and biodiversity in urban ecosystems. The objectives of this study were to: (1) describe the arthropod communities present within the grasslands on the John F. Kennedy International Airport and (2) assess spatial and temporal variation in those arthropod communities. We collected arthropods using a vacuum sampler during 2003 and using sweep-net collection methods during 2003 and 2004. During 2003, a total of 1,467 arthropods, representing 17 orders and 68 families were found in vacuum samples. A total of 3,784 arthropods, representing 12 orders and 94 families were collected in sweep-net samples during 2003. In 2004, a total of 3,281 arthropods, representing 12 orders and 85 families were collected in sweep-net samples. Hemiptera, Orthoptera, and Diptera were the most abundant taxa, accounting for 47, 18, and 14% of all arthropods captured, respectively. We found evidence of spatial and temporal variation in arthropod abundance, in particular as noted by fluctuations in Orthoptera: Acrididae and Hemiptera: Auchenorrhyncha. Hemipteran family diversity was also influenced by habitat type. Grassland habitats on airfields, although influenced by anthropogenic factors (e.g., mowing), have the potential to provide abundant and diverse arthropod communities and might serve as a refugium for such species within urban ecosystems. C1 [Kutschbach-Brohl, Lisa; Washburn, Brian E.; Bernhardt, Glen E.] Natl Wildlife Res Ctr, USDA APHIS WS, Sandusky, OH 44870 USA. [Chipman, Richard B.] USDA APHIS WS, Castleton, NY 12033 USA. [Francoeur, Laura C.] Port Authority NY & NJ, Jamaica, NY 11430 USA. RP Washburn, BE (reprint author), Natl Wildlife Res Ctr, USDA APHIS WS, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU Port Authority of New York and New Jersey; New Jersey and United States Department Agriculture; Plant Health Inspection Service-Wildlife Services FX This research effort was funded by the Port Authority of New York and New Jersey and United States Department Agriculture-Animal and Plant Health Inspection Service-Wildlife Services. We thank various members of the USDA-APHIS-Wildlife Services New York staff, especially D. Slater and A. Gosser, and the JFKIA Airfield Operations staff, especially S. Nowak, for their assistance in the field. In addition, we thank J. Dierker and S. Johnston for assistance with data entry. T. DeVault, T. Seamans, and 2 anonymous reviewers kindly provided helpful comments on earlier versions of this manuscript. NR 81 TC 10 Z9 11 U1 6 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1366-638X J9 J INSECT CONSERV JI J. Insect Conserv. PD AUG PY 2010 VL 14 IS 4 BP 347 EP 358 DI 10.1007/s10841-010-9264-8 PG 12 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA 617VU UT WOS:000279310800003 ER PT J AU Shearer, GC Harris, WS Pedersen, TL Newman, JW AF Shearer, Gregory C. Harris, William S. Pedersen, Theresa L. Newman, John W. TI Detection of omega-3 oxylipins in human plasma and response to treatment with omega-3 acid ethyl esters SO JOURNAL OF LIPID RESEARCH LA English DT Article DE omega-3 fatty acid; P-OM3; inflammation; EPA; DHA ID POLYUNSATURATED FATTY-ACIDS; SOLUBLE EPOXIDE HYDROLASE; EICOSAPENTAENOIC ACID; DOUBLE-BLIND; FISH OILS; OMEGA-3-FATTY-ACIDS; PREVENTION; MECHANISM; RECEPTOR; RISK AB The long-chain omega-3 fatty acids (n-3 FA) eicosapentaenoic acid (EPA) and docosahexaenoic acids (DHA) have beneficial health effects, but the molecular mediators of these effects are not well characterized. Oxygenated n-3 FAs (oxylipins) may be an important class of mediators. Members of this chemical class include epoxides, alcohols, diols, and ketones, many of which have bioactivity in vitro. Neither the presence of n-3 oxylipins in human plasma nor the effect of n-3 FA ingestion on their levels has been documented. We measured plasma oxylipins derived from both the n-3 and n-6 FA classes in healthy volunteers (n = 10) before and after 4 weeks of treatment with prescription n-3 FA ethyl esters (4 g/day). At baseline, EPA and DHA oxylipins were detected in low (1-50 nM) range, with alcohols > epoxides >= diols. Treatment increased n-3 oxylipin levels 2- to 5-fold and reduced selected n-6 oxylipins by similar to 20%.Jlr This is the first documentation that endogenous n-3 oxylipin levels can be modulated by n-3 FA treatment in humans. The extent to which the beneficial cardiovascular effects of n-3 FAs are mediated by increased n-3 and/or reduced n-6 oxylipin levels remains to be explored.-Shearer, G. C., W. S. Harris, T. L. Pedersen, and J. W. Newman. Detection of omega-3 oxylipins in human plasma and response to treatment with omega-3 acid ethyl esters. J. Lipid Res. 2010. 51: 2074-2081. C1 [Shearer, Gregory C.; Harris, William S.] Sanford Res USD, Cardiovasc Hlth Res Ctr, Sioux Falls, SD USA. [Pedersen, Theresa L.; Newman, John W.] Univ Calif Davis, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Shearer, GC (reprint author), Sanford Res USD, Cardiovasc Hlth Res Ctr, Sioux Falls, SD USA. EM greg.shearer@usd.edu FU Agricultural Research Service (ARS) [5306-51530-016-00D]; U.S. Department of Agriculture (USDA); National Institutes of Health; IDeA Networks of Biomedical Research Excellence (INBRE), National Center for Research Resources [2-P20-RR016479] FX This work was supported in part by Project 5306-51530-016-00D from the Agricultural Research Service (ARS), U.S. Department of Agriculture (USDA); by the National Institutes of Health; and by Grant 2-P20-RR016479 from the IDeA Networks of Biomedical Research Excellence (INBRE) Program, National Center for Research Resources. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health or the U.S. Department of Agriculture. NR 40 TC 66 Z9 68 U1 1 U2 20 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 J9 J LIPID RES JI J. Lipid Res. PD AUG PY 2010 VL 51 IS 8 BP 2074 EP 2081 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 625LM UT WOS:000279896800005 PM 19671931 ER PT J AU Pyare, S Smith, WP Shanley, CS AF Pyare, Sanjay Smith, Winston P. Shanley, Colin S. TI Den use and selection by northern flying squirrels in fragmented landscapes SO JOURNAL OF MAMMALOGY LA English DT Article DE Alexander Archipelago; cavities; clear-cut logging; den use; fragmentation; matrix; Prince of Wales Island; Southeast Alaska; temperate rain forest ID TEMPERATE RAIN-FOREST; CENTRAL APPALACHIAN MOUNTAINS; OLD-GROWTH FORESTS; NEST-TREE USE; GLAUCOMYS-SABRINUS; SOUTHEAST ALASKA; PTEROMYS-VOLANS; PACIFIC-NORTHWEST; WESTERN HEMLOCK; SIERRA-NEVADA AB We studied den use and den-habitat selection by the Prince of Wales Island flying squirrel (Glaucomys sabrinus griseifrons) at multiple spatial scales in fragmented temperate rain-forest habitats because of the role dens play in the distribution, reproduction, and population density of this endemic subspecies. We observed differences in spatial patterns associated with den use between juveniles and adults: juvenile core denning areas were almost an order of magnitude larger than those of adults, and juveniles used about one-half the number of dens per month as adults. Female juveniles exhibited both the largest mean and maximum movements between consecutive dens among all age and sex classes. At the microhabitat scale of den selection snags were not selected over live trees, but flying squirrels primarily used cavities in snags and live trees. Flying squirrels also selected dens in the largest diameter live trees and snags, in snags with intermediate levels of decay, in live trees with more conks and visible bole entries, and in western hemlock (Tsuga heterophylla). At the broader scale of den selection flying squirrels chose den locations in neighborhoods with higher-volume forests and lower levels of fragmentation, although not lower absolute amounts of edge, than was available across the landscape. Our results suggest that extensive modification of landscapes from clear-cut logging and the creation of an early-seral matrix appeared to influence spatial patterns of den use in flying squirrels and den selection at the broader scale. When compared to patterns in a more-intact landscape, den selection at the microhabitat scale did not relate to differences in landscape context, suggesting additional factors might play an important role in den use across the region. DOI: 10.1644/09-MAMM-A-179.1. C1 [Pyare, Sanjay] Univ Alaska SE, Environm Sci & Geog Program, Juneau, AK 99801 USA. [Smith, Winston P.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Olympia, WA 98512 USA. [Shanley, Colin S.] Univ Alaska, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. RP Pyare, S (reprint author), Univ Alaska SE, Environm Sci & Geog Program, 11120 Glacier Highway, Juneau, AK 99801 USA. EM sanjay.pyare@uas.alaska.edu FU United States Fish and Wildlife Service; Denver Zoological Foundation; Earthwatch Foundation; University of Alaska Southeast FX We thank the United States Fish and Wildlife Service, the Denver Zoological Foundation, the Earthwatch Foundation, and the University of Alaska Southeast for funding and supporting this study. R. Edwards, Juneau Aquatic-Land Interactions Team, Pacific Northwest Research Station, and the staff of Thorne Bay Ranger District, especially K. Webb, provided invaluable logistic support. V. Bakker and K. Hastings reviewed an early draft of this manuscript and offered insightful comments. We appreciate the thorough reviews of 2 anonymous referees whose constructive comments ultimately improved the quality of our paper. NR 65 TC 11 Z9 11 U1 0 U2 16 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 J9 J MAMMAL JI J. Mammal. PD AUG PY 2010 VL 91 IS 4 BP 886 EP 896 DI 10.1644/09-MAMM-A-179.1 PG 11 WC Zoology SC Zoology GA 642LM UT WOS:000281215100012 ER PT J AU Davis, JC Castleberry, SB Kilgo, JC AF Davis, Justin C. Castleberry, Steven B. Kilgo, John C. TI Influence of coarse woody debris on the soricid community in southeastern Coastal Plain pine stands SO JOURNAL OF MAMMALOGY LA English DT Article DE Blarina; coarse woody debris; Cryptotis; decay state; shrew; Sorex; topographic variables; upland pine ID SMALL MAMMALS; SOREX-LONGIROSTRIS; FOREST; SHREWS; RESPONSES; CINEREUS; ECOLOGY; YOUNG AB Shrew abundance has been linked to the presence of coarse woody debris (CWD), especially downed logs, in many regions in the United States. We investigated the importance of CWD to shrew communities in managed upland pine stands in the southeastern United States Coastal Plain. Using a randomized complete block design, 1 of the following treatments was assigned to twelve 9.3-ha plots: removal (n = 3; all downed CWD >= 10 cm in diameter and >= 60 cm long removed), downed (n = 3; 5-fold increase in volume of downed CWD), snag (n = 3; 10-fold increase in volume of standing dead CWD), and control (n = 3; unmanipulated). Shrews (Blarina carolinensis, Sorex longirostris, and Cryptotis parva) were captured over 7 seasons from January 2007 to August 2008 using drift-fence pitfall trapping arrays within treatment plots. Topographic variables were measured and included as treatment covariates. More captures of B. carolinensis were made in the downed treatment compared to removal, and captures of S. longirostris were greater in downed and snag compared to removal. Captures of C. parva did not differ among treatments. Captures of S. longirostris were positively correlated with slope. Our results suggest that abundance of 2 of the 3 common shrew species of the southeastern Coastal Plain examined in our study is influenced by the presence of CWD. DOI: 10.1644/09MAMM-A-170.1. C1 [Castleberry, Steven B.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Davis, Justin C.] Fitzhugh Carter Tract Econfina Creek Wildlife Man, Florida Fish & Wildlife Conservat Commiss, Chipley, FL 32428 USA. [Kilgo, John C.] US Forest Serv Savannah River, So Res Stn, USDA, New Ellenton, SC 29809 USA. RP Castleberry, SB (reprint author), Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM scastle@warnell.uga.edu FU United States Department of Energy Savannah River Operations Office [DE-A109-00SR22188] FX We acknowledge J. Blake, M. Dorcas, S. Loeb, and J. Laerm (deceased) who initiated this project. M. Vukovich, K. Wright, and E. Olson provided logistical assistance throughout the project. We thank J. Linehan, A. McKee, D. Sollenberger, M. Goode, and M. Murphy for field assistance and E. Reed for laboratory assistance in aging shrews. S. Albeke provided guidance in geographic information system applications and analysis, and S. Ellis-Felege and R. J. Cooper assisted with statistical analysis. Funding was provided by the United States Department of Energy Savannah River Operations Office through the United States Forest Service_Savannah River under Interagency Agreement DE-A109-00SR22188. NR 56 TC 8 Z9 8 U1 2 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD AUG PY 2010 VL 91 IS 4 BP 993 EP 999 DI 10.1644/09-MAMM-A-170.1 PG 7 WC Zoology SC Zoology GA 642LM UT WOS:000281215100022 ER PT J AU Solaiman, DKY Swingle, BM Ashby, RD AF Solaiman, Daniel K. Y. Swingle, Bryan M. Ashby, Richard D. TI A new shuttle vector for gene expression in biopolymer-producing Ralstonia eutropha SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Green fluorescent protein; Poly(hydroxyalkanoate); Alpha-galactosidase; Tetracycline resistance ID BETA-HYDROXYBUTYRIC ACID; SOY MOLASSES; PHA SYNTHASE; PSEUDOMONADS AB Ralstonia eutropha (formerly Alcaligenes eutrophus) is a fascinating microorganism with a great scientific importance and an immense commercial potential. A new genetic transformation system for the organism would greatly facilitate the biological study and molecular engineering of this organism. We report here a versatile gene expression method for the genetic engineering of R. eutropha. This method, based on a simplified electroporation protocol, uses a recombinant plasmid, pBS29-P2, containing a Pseudomonas syringae promoter (P2) and two antibiotic-resistance markers (i.e., genes coding for kanamycin (Km)- and tetracycline (Tc)-resistance). Using this method, we successfully achieved transformation of wild-type R. eutropha and its poly(hydroxyalkanoate)-negative mutant, R. eutropha PHB(-)4, with various pBS29-P2-based recombinants. A transformation frequency as high as 4 x 10(3) Km-resistance colonies/mu g DNA was obtained per electroporation experiment. We further demonstrated the successful expression of a heterologous gene coding for green-fluorescent-protein by fluorescence measurement. In addition, our results indicated the expression of a truncated but active Streptomyces coelicolor alpha-galactosidase in R. eutropha. Published by Elsevier B.V. C1 [Solaiman, Daniel K. Y.; Ashby, Richard D.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Swingle, Bryan M.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Solaiman, DKY (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM dan.solaiman@ars.usda.gov NR 23 TC 2 Z9 2 U1 0 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 J9 J MICROBIOL METH JI J. Microbiol. Methods PD AUG PY 2010 VL 82 IS 2 BP 120 EP 123 DI 10.1016/j.mimet.2010.04.010 PG 4 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 629RN UT WOS:000280217500002 PM 20447426 ER PT J AU Escobar, J Frank, JW Suryawan, A Nguyen, HV Van Horn, CG Hutson, SM Davis, TA AF Escobar, Jeffery Frank, Jason W. Suryawan, Agus Nguyen, Hanh V. Van Horn, Cynthia G. Hutson, Susan M. Davis, Teresa A. TI Leucine and alpha-Ketoisocaproic Acid, but Not Norleucine, Stimulate Skeletal Muscle Protein Synthesis in Neonatal Pigs SO JOURNAL OF NUTRITION LA English DT Article ID CHRONIC KIDNEY-DISEASE; CHAIN AMINO-ACIDS; TRANSLATION INITIATION; KETO-ACIDS; INSULIN; METABOLISM; KINASE; TISSUE; MTOR; RATS AB The branched-chain amino acid, leucine, acts as a nutrient signal to stimulate protein synthesis in skeletal muscle of young pigs. However, the chemical structure responsible for this effect has not been identified. We have shown that the other branched-chain amino acids, isoleucine and valine, are not able to stimulate protein synthesis when raised in plasma to levels within the postprandial range. In this study, we evaluated the effect of leucine, alpha-ketoisocaproic acid (KIC), and norleucine infusion (0 or 400 mu mol.kg(-1).h(-1) for 60 min) on protein synthesis and activation of translation initiation factors in piglets. Infusion of leucine, KIC, and norleucine raised plasma levels of each compound compared with controls. KIC also increased (P < 0.01) and norleucine reduced (P < 0.02) plasma levels of leucine compared with controls. Administration of leucine and KIC resulted in greater (P < 0.006) phosphorylation of eukaryotic initiation factor (eIF) 4E binding protein-1 (4E-BP1) and eIF4G, lower (P < 0.04) abundance of the inactive 4E-BP1.eIF4E complex, and greater (P < 0.05) active eIF4G.eIF4E complex formation in skeletal muscle compared with controls. Protein synthesis in skeletal muscle was greater (P < 0.02) in leucine- and KIC-infused pigs than in those in the control group. Norleucine infusion did not affect muscle protein synthesis or translation initiation factor activation. In liver, neither protein synthesis nor activation of translation initiation factors was affected by treatment. These results suggest that the ability of leucine to act as a nutrient signal to stimulate skeletal muscle protein synthesis is specific for leucine and/or its metabolite, KIC. J. Nutr. 140:1418-1424, 2010. C1 [Escobar, Jeffery; Frank, Jason W.; Suryawan, Agus; Nguyen, Hanh V.; Davis, Teresa A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Van Horn, Cynthia G.; Hutson, Susan M.] Wake Forest Univ, Bowman Gray Sch Med, Dept Biochem, Winston Salem, NC 27157 USA. RP Davis, TA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM tdavis@bcm.edu FU NIH [AR-44474, T32 HD-07445]; USDA/Agricultural Research Service (USDA/ARS) [6250510000-33] FX Supported in part by NIH grants AR-44474 (TA. Davis) and by the USDA/Agricultural Research Service (USDA/ARS) under Cooperative Agreement no. 6250510000-33 (TA. Davis). This research was also supported in part by NIH Training grant T32 HD-07445. This work is a publication of the USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine. The contents of this publication do not necessarily reflect the views or politics of the USDA, nor does the mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. NR 35 TC 31 Z9 33 U1 0 U2 7 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2010 VL 140 IS 8 BP 1418 EP 1424 DI 10.3945/jn.110.123042 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 629PE UT WOS:000280211300006 PM 20534881 ER PT J AU Marini, JC Didelija, IC Castillo, L Lee, B AF Marini, Juan C. Didelija, Inka Cajo Castillo, Leticia Lee, Brendan TI Plasma Arginine and Ornithine Are the Main Citrulline Precursors in Mice Infused with Arginine-Free Diets SO JOURNAL OF NUTRITION LA English DT Article ID PORTAL-DRAINED VISCERA; SMALL-INTESTINE; METABOLISM; FLUX; DEFICIENCY; PROLINE; TRANSCARBAMYLASE; GLUTAMINE; HUMANS; ENZYME AB Dietary arginine is the main dietary precursor for citrulline synthesis, but it is not known if other precursors can compensate when arginine is absent in the diet. To address this question, the contributions of plasma and dietary precursors were determined by using multitracer protocols in conscious mice infused i.g. either an arginine-sufficient diet [Arg(+)] or an arginine-free diet [Arg(-)]. The plasma entry rate of citrulline and arginine did not differ between the 2 diet groups (156 +/- 6 and 564 +/- 30 mu mol.kg(-1).h(-1), respectively); however, the entry rate of ornithine was greater in the mice fed the Arg(+) than the Arg(-) diet (332 +/- 33 vs. 180 +/- 16 mu mol.kg(-1).h(-1)). There was a greater utilization of plasma ornithine for the synthesis of citrulline (49 +/- 4 vs. 36 +/- 3 mu mol.kg(-1).h(-1), 30 +/- 3% vs. 24 +/- 2% of citrulline entry rate) in the mice fed the Arg(-) diet than the Arg(+) diet. The utilization of plasma arginine did not differ between the 2 diet groups for citrulline synthesis, either through plasma ornithine (similar to 29 +/- 3 mu mol.kg(-1).h(-1)) or at the site of citrulline synthesis (similar to 12 +/- 3 mu mol.kg(-1).h(-1)). The contribution of dietary proline to the synthesis of citrulline was mainly at the site of citrulline production (17 +/- 1 mu mol.kg(-1).h(-1)), rather than through plasma ornithine (5 +/- 0.4 mu mol.kg(-1).h(-1)). Dietary glutamine was utilized only at the site of citrulline synthesis (4 +/- 0.2 mu mol.kg(-1).h(-1)). Dietary glutamine and proline made a greater contribution to the synthesis of citrulline in mice fed the Arg(-) diet but remained minor sources for citrulline production. Plasma arginine and ornithine are able to support citrulline synthesis during arginine-free feeding. J. Nutr. 140: 1432-1437, 2010. C1 [Marini, Juan C.; Didelija, Inka Cajo; Castillo, Leticia] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Lee, Brendan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Lee, Brendan] Howard Hughes Med Inst, Houston, TX 77030 USA. RP Marini, JC (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM marini@bcm.edu FU USDA [2533771314]; NIH [KO1 RR24173] FX Supported by USDA grant no. 2533771314 and NIH grant no. KO1 RR24173. NR 31 TC 9 Z9 9 U1 0 U2 2 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2010 VL 140 IS 8 BP 1432 EP 1437 DI 10.3945/jn.110.125377 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 629PE UT WOS:000280211300008 PM 20573946 ER PT J AU Pae, M Ren, ZH Meydani, M Shang, F Meydani, SN Wu, DY AF Pae, Munkyong Ren, Zhihong Meydani, Mohsen Shang, Fu Meydani, Simin Nikbin Wu, Dayong TI Epigallocatechin-3-Gallate Directly Suppresses T Cell Proliferation through Impaired IL-2 Utilization and Cell Cycle Progression SO JOURNAL OF NUTRITION LA English DT Article ID NF-KAPPA-B; GREEN TEA; INTERLEUKIN-2 RECEPTOR; PROTEASOME INHIBITORS; PHYSICAL INTERACTION; ACCESSORY CELLS; SOLUBLE FACTORS; IFN-GAMMA; IN-VITRO; GALLATE AB Previously, we demonstrated that in vitro epigallocatechin-3-gallate (EGCG) supplementation inhibited T cell response in mouse spleen cells. In this study, we confirmed this effect of EGCG in mice fed 0.3% EGCG for 6 wk. A coculture with all the combinations of preincubating antigen-presenting cells and T cells with or without EGCG showed that EGCG suppressed antigen-induced T cell proliferation, mainly through a direct effect on T cells. To determine the mechanisms for this effect of EGCG, we stimulated purified mouse T cells with anti-CD3/CD28 in the presence of EGCG (2.5-15 mu mol/L) and found that EGCG dose-dependently inhibited cell division and cell cycle progression and this effect of EGCG was more pronounced in CD4(+) than in CD8(+) T cells. Interleukin (IL)-2 concentrations in EGCG-treated cell cultures showed no difference up to 24 h but were higher in the cultures at 48 h compared with the untreated control cells. However, intracellular staining showed no difference between EGCG-treated and untreated control cells in IL-2 synthesis, but EGCG-treated cells expressed less IL-2 receptor (IL-2R) compared with untreated control cells. EGCG did not affect mRNA expression of IL-2 and IL-2R. These results indicate that EGCG-induced IL-2 accumulation in 48 h cultures is due to its reduced utilization. In summary, EGCG directly inhibits T cell proliferative response to both polyclonal and antigen-specific stimulation. CD4(+) cells are more responsive to EGCG than CD8(+) cells. Future studies should determine the effect of EGCG on CD4(+) cell subsets to assess its application in T cell-mediated autoimmune diseases. J. Nutr. 140: 1509-1515, 2010. C1 [Pae, Munkyong; Ren, Zhihong; Meydani, Simin Nikbin; Wu, Dayong] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. [Meydani, Mohsen] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Vasc Biol Lab, Boston, MA 02111 USA. [Shang, Fu] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. RP Wu, DY (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. EM dayong.wu@tufts.edu RI Pae, Munkyong/B-4831-2012 FU USDA, Agriculture Research Service [58-1950-7-707] FX Supported by the USDA, Agriculture Research Service under contract number 58-1950-7-707. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 37 TC 22 Z9 22 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD AUG PY 2010 VL 140 IS 8 BP 1509 EP 1515 DI 10.3945/jn.110.124743 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 629PE UT WOS:000280211300019 PM 20534878 ER PT J AU Butte, NE Wong, WW Adolph, AL Puyau, MR Vohra, FA Zakeri, IF AF Butte, Nancy E. Wong, William W. Adolph, Anne L. Puyau, Maurice R. Vohra, Firoz A. Zakeri, Issa F. TI Validation of Cross-Sectional Time Series and Multivariate Adaptive Regression Splines Models for the Prediction of Energy Expenditure in Children and Adolescents Using Doubly Labeled Water SO JOURNAL OF NUTRITION LA English DT Article ID COMBINED HEART-RATE; MOTION SENSOR TECHNIQUE; PHYSICAL-ACTIVITY; MOVEMENT SENSOR; YOUNG-CHILDREN; ACCELEROMETRY; VALIDITY; ACTIHEART; HUMANS AB Accurate, nonintrusive, and inexpensive techniques are needed to measure energy expenditure (EE) in free-living populations. Our primary aim in this study was to validate cross-sectional time series (CSTS) and multivariate adaptive regression splines (MARS) models based on observable participant characteristics, heart rate (HR), and accelerometer counts (AC) for prediction of minute-by-minute EE, and hence 24-h total EE (TEE), against a 7-d doubly labeled water (DLW) method in children and adolescents. Our secondary aim was to demonstrate the utility of CSTS and MARS to predict awake EE, sleep EE, and activity EE (AEE) from 7-d HR and AC records, because these shorter periods are not verifiable by DLW, which provides an estimate of the individual's mean TEE over a 7-d interval. CSTS and MARS models were validated in 60 normal-weight and overweight participants (ages 5-18 y). The Actiheart monitor was used to simultaneously measure HR and AC. For prediction of TEE, mean absolute errors were 10.7 +/- 307 kcal/d and 18.7 +/- 252 kcal/d for CSTS and MARS models, respectively, relative to DLW. Corresponding root mean square error values were 305 and 251 kcal/d for CSTS and MARS models, respectively. Bland-Altman plots indicated that the predicted values were in good agreement with the DLW-derived TEE values. Validation of CSTS and MARS models based on participant characteristics, HR monitoring, and accelerometry for the prediction of minute-by-minute EE, and hence 24-h TEE, against the DLW method indicated no systematic bias and acceptable limits of agreement for pediatric groups and individuals under free-living conditions. J. Nutr. 140: 1516-1523, 2010. C1 [Butte, Nancy E.; Wong, William W.; Adolph, Anne L.; Puyau, Maurice R.; Vohra, Firoz A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Zakeri, Issa F.] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19102 USA. RP Butte, NE (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM nbutte@bcm.edu FU NIH [DK-74387]; USDA/Agricultural Research Service [6250-51000-037] FX Supported by federal funds from the NIH (DK-74387) and by the USDA/Agricultural Research Service under Cooperative Agreement number 6250-51000-037. The contents of this publication do not necessarily reflect the views or policies of the NIH or USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. NR 41 TC 23 Z9 25 U1 0 U2 4 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2010 VL 140 IS 8 BP 1516 EP 1523 DI 10.3945/jn.109.120162 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 629PE UT WOS:000280211300020 PM 20573939 ER PT J AU Freedman, LS Guenther, PM Krebs-Smith, SM Dodd, KW Midthune, D AF Freedman, Laurence S. Guenther, Patricia M. Krebs-Smith, Susan M. Dodd, Kevin W. Midthune, Douglas TI A Population's Distribution of Healthy Eating Index-2005 Component Scores Can Be Estimated When More Than One 24-Hour Recall Is Available SO JOURNAL OF NUTRITION LA English DT Article ID EPISODICALLY CONSUMED FOODS AB The USDA's Healthy Eating Index-2005 (HEI-2005) is a tool to quantify the quality of diet consumed by individuals in the U.S. It comprises 12 components expressed as ratios of a food group or nutrient intake to energy intake. Components are scored on a scale from 0 to M, where M is 5, 10, or 20. Ideally, the HEI-2005 is calculated on the basis of the usual, or long-term average, dietary intake of an individual. In recent cycles of the NHANES, intake data have been collected via 24-h recalls for more than 1 d on most participants. We present here a statistical method to estimate a population's distribution of usual HEI-2005 component scores when >= 2 d of dietary information is available for a sample of individuals from the population. Distributions for the total population and for age-gender subgroups may be estimated. The method also yields an estimate of the population's mean total HEI-2005 score. Application of the method to NHANES data for 2001-2004 yielded estimated distributions for all 12 components; those of total vegetables (range 0-5), whole grains (range 0-5), and energy from solid fats, alcoholic beverages, and added sugars (range 0-20) are presented. The total population mean scores for these components were 3.21, 1.00, and 8.41, respectively. An estimated 30% of the total population had a score of <2.5 for total vegetables. This is the first time, to our knowledge, that estimated distributions of usual HEI-2005 component scores have been published. J. Nutr. 140: 1529-1534, 2010. C1 [Freedman, Laurence S.] Gertner Inst Epidemiol & Hlth Policy Res, IL-52161 Tel Hashomer, Israel. [Guenther, Patricia M.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. [Krebs-Smith, Susan M.; Dodd, Kevin W.; Midthune, Douglas] NCI, Bethesda, MD 20892 USA. RP Freedman, LS (reprint author), Gertner Inst Epidemiol & Hlth Policy Res, IL-52161 Tel Hashomer, Israel. EM lsf@actcom.co.il FU Information Management Services Inc with the US National Cancer Institute; USDA; National Cancer Institute FX Supported by a contract held by Information Management Services Inc with the US National Cancer Institute (to L.S.F.); the remaining authors were supported by their respective institutions, the USDA, and the National Cancer Institute. NR 10 TC 22 Z9 23 U1 2 U2 4 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD AUG PY 2010 VL 140 IS 8 BP 1529 EP 1534 DI 10.3945/jn.110.124594 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 629PE UT WOS:000280211300022 PM 20573940 ER PT J AU Dubey, JP Rajendran, C Costa, DGC Ferreira, LR Kwok, OCH Qu, D Su, C Marvulo, MFV Alves, LC Mota, RA Silva, JCR AF Dubey, J. P. Rajendran, C. Costa, D. G. C. Ferreira, L. R. Kwok, O. C. H. Qu, D. Su, C. Marvulo, M. F. V. Alves, L. C. Mota, R. A. Silva, J. C. R. TI NEW TOXOPLASMA GONDII GENOTYPES ISOLATED FROM FREE-RANGE CHICKENS FROM THE FERNANDO DE NORONHA, BRAZIL: UNEXPECTED FINDINGS SO JOURNAL OF PARASITOLOGY LA English DT Article ID IDENTIFICATION AB Worldwide comparison of Toxoplasma gondii isolates from free-range chickens (Gallus domesticus) has indicated that T. gondii isolates from Brazil are phenotypically and genetically different from isolates from other countries; most strains from Brazil are pathogenic to mice, there is great genetic variability, most isolates are nonclonal, and Type II is absent or rare. The prevalence of T. gondii in 50 free-range chickens from the island of Fernando de Noronha, Brazil (this island is 350 km from the mainland) was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT); 42 (84%) chickens had titers of 1:5 in 2, 1:10 in 4, 1:20 in 3, 1:40 in 6, 1:80 in 6, 1:160 in 5, 1:320 in 3, and 1:640 or higher in 13 chickens. Hearts of 40 seropositive chickens were bioassayed individually in mice. Toxoplasma gondii was isolated from 24 chickens with MAT titers of 1:5 or higher; the isolates were designated TgCKBr210-233. None of the isolates was pathogenic for mice. The restricted fragment length polymorphism using 10 markers revealed 6 genotypes, including the Type II, Type III, and 4 new chicken genotypes (#59-#62) that were different from genotypes so far reported in Brazil. All 24 isolates were successfully genotyped; 15 isolates were Brazil chicken type #59, 1 type #60, 1 type #61, 1 type #62; 5 were Type II (with Type I allele at the Apico locus); and 1 isolate was clonal Type III. Results in this study indicate that T. gondii on this island consists of unique genotypes as well as clonal genotypes that are dominant in Europe and North America. C1 [Dubey, J. P.; Rajendran, C.; Costa, D. G. C.; Ferreira, L. R.; Kwok, O. C. H.; Qu, D.; Su, C.; Marvulo, M. F. V.; Alves, L. C.; Mota, R. A.; Silva, J. C. R.] USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Su, Chunlei/M-1892-2013 OI Su, Chunlei/0000-0001-8392-7108 NR 16 TC 32 Z9 36 U1 0 U2 4 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 709 EP 712 DI 10.1645/GE-2425.1 PG 4 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800006 PM 20486738 ER PT J AU Dubey, JP Rajendran, C Ferreira, LR Kwok, OCH Sinnett, D Majumdar, D Su, C AF Dubey, J. P. Rajendran, C. Ferreira, L. R. Kwok, O. C. H. Sinnett, D. Majumdar, D. Su, C. TI A NEW ATYPICAL HIGHLY MOUSE VIRULENT TOXOPLASMA GONDII GENOTYPE ISOLATED FROM A WILD BLACK BEAR IN ALASKA SO JOURNAL OF PARASITOLOGY LA English DT Article ID FREE-RANGE CHICKENS; CONGENITAL TOXOPLASMOSIS; MICROSATELLITE ANALYSIS; MICE; CHROMOSOME; MARKERS; BRAZIL AB Most strains of Toxoplasma gondii isolated in North America and Europe are grouped into 3 (Types I, II, III) genotypes and are considered clonal. Recent evidence suggests that illness due to toxoplasmosis in immunocompetent persons may be related to infection with an atypical genotype; these strains are mouse virulent. In the present study, a new mouse-virulent atypical T gondii genotype was isolated from an asymptomatic black bear (Ursus americanus) from Alaska. The bear had a titer of 1:1,600 using the modified agglutination test for T. gondii. Swiss Webster out-bred mice inoculated with bear heart homogenate died of acute toxoplasmosis, 12 days post-inoculation (PI). Cats fed tissues from chronically infected animals (day 30 PI) shed oocysts, but only 1 of 3 cats fed acutely infected mice (12, 16, 18 days PI) shed oocysts. The isolate (designated TgBbUS1) was mouse virulent; mice inoculated with 1 oocyst or 1 tachyzoite died of acute toxoplasmosis. The restricted fragment length polymorphism using 10 markers revealed that the strain possessed an atypical genotype: type I allele at loci SAG1, (5'-3')SAG2, SAG3, c22-8, c29-2, L358, and Apico; type II allele at locus alt.SAG2; and type III allele at loci BTUB, GRA6, and PK1. DNA sequencing at intron loci EF1, HP2, and UPRT1 revealed that the TgBbUS1 is a divergent T. gondii strain. These results indicate that mouse-virulent atypical T. gondii genotypes are also circulating in wildlife in North America. C1 [Dubey, J. P.; Rajendran, C.; Ferreira, L. R.; Kwok, O. C. H.; Sinnett, D.; Majumdar, D.; Su, C.] USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Su, Chunlei/M-1892-2013 OI Su, Chunlei/0000-0001-8392-7108 NR 25 TC 21 Z9 22 U1 0 U2 4 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 713 EP 716 DI 10.1645/GE-2429.1 PG 4 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800007 PM 20486739 ER PT J AU Feng, XS Zhang, NS Tuo, WB AF Feng, Xiaosheng Zhang, Naisheng Tuo, Wenbin TI NEOSPORA CANINUM TACHYZOITE- AND ANTIGEN-STIMULATED CYTOKINE PRODUCTION BY BONE MARROW-DERIVED DENDRITIC CELLS AND SPLEEN CELLS OF NAIVE BALB/C MICE SO JOURNAL OF PARASITOLOGY LA English DT Article ID NATURAL-KILLER-CELLS; CD4(+) T-CELLS; IMMUNE-RESPONSES; GAMMA-INTERFERON; TOXOPLASMA-GONDII; INFECTION; CATTLE; IDENTIFICATION; IL-12; INTERLEUKIN-10 AB Neospora caninum is an intracellular protozoan pathogen that causes abortion in cattle. This parasite elicits a typical type 1 immune response in host animals, and it is widely believed that the strong type 1 immune response during pregnancy may result in fetal death. Pro-inflammatory and/or inflammatory cytokines produced during either primary or secondary pathogen exposure are supposed to be the mediators of abortion. The present study defined cytokine production by murine naive dendritic cells and spleen cells in response to whole Neospora tachyzoites (live, heat-killed, freeze-killed) or whole-cell tachyzoite lysate in the form of total (NcAg), soluble (sNcAg), or insoluble antigen (isNcAg). All tachyzoite and antigen preparations at high doses stimulated high levels of interleukin (IL) -12, interferon (IFN) -gamma, and tumor necrosis factor (TNF) -alpha, except for heat-killed tachyzoites and sNcAg, which induced moderate level of IL-12 and very low levels of IFN-gamma and TNF-alpha. In general, whole N. caninum tachyzoites were more effective in inducing IL-12, IFN-gamma, and TNF-alpha than the lysate antigen preparations. It appears that the heat-killed N. caninum tachyzoites were less potent in eliciting IFN-gamma or IL-10, but more effective in inducing IL-4. Thus, heat-inactivated tachyzoites or sNcAg alone may not be powerful enough to elicit strong type 1 immune responses against the disease. The present study comprehensively studied the production of critical cytokine by the murine dendritic cells and spleen cells in response to N. caninum; these results may facilitate a better understanding of antigen priming and aid in the design of vaccines/adjuvants against neosporosis. C1 [Feng, Xiaosheng; Zhang, Naisheng; Tuo, Wenbin] USDA ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Tuo, WB (reprint author), USDA ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM wenbin.tuo@ars.usda.gov NR 32 TC 9 Z9 9 U1 0 U2 2 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 717 EP 723 DI 10.1645/GE-1900.1 PG 7 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800008 PM 20738198 ER PT J AU Dubey, JP Rosenthal, BM Felix, TA AF Dubey, J. P. Rosenthal, B. M. Felix, T. A. TI MORPHOLOGIC AND MOLECULAR CHARACTERIZATION OF THE SARCOCYSTS OF SARCOCYSTIS RILEYI (APICOMPLEXA: SARCOCYSTIDAE) FROM THE MALLARD DUCK (ANAS PLATYRHYNCHOS) SO JOURNAL OF PARASITOLOGY LA English DT Article ID SP PROTOZOA; WESTERN CANADA; NEURONA; OPOSSUM; BIRDS AB Macroscopic sarcocysts are often observed in ducks, but at present their taxonomic status remains uncertain because ducks serve as intermediate hosts for several such parasites in the genus Sarcocystis. One such species, Sarcocystis rileyi, was long ago established to involve the northern shoveler duck (Anas clypeata) and the striped skunk (Mephitis mephitis) as its intermediate and definitive hosts, respectively. Here, we employed light microscopy, electron microscopy, and DNA sequencing to more precisely describe diagnostic attributes of parasites presumed to represent S. rileyi occurring in a naturally-infected mallard duck (Anas platyrhynchos). By light and transmission electron microscopy, sarcocysts from the mallard duck resembled the S. rileyi described from A. clypeata. We document 18S, ITS-1, and 28S rDNA sequences from the mallard duck, the first for S. rileyi from any host. Sequences of conserved and variable portions of nuclear ribosomal DNA indicated that S. rileyi is related to, but distinct from, parasites employing opossums as their definitive host (including Sarcocystis neurona and Sarcocystis falcatula). Diagnostic ultrastructural features and nucleotide sequences should aid in future studies and communications regarding this parasitic taxon, which lends itself to experimentation because its sarcocysts are macroscopic and easily excised from infected birds. C1 [Dubey, J. P.; Rosenthal, B. M.; Felix, T. A.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 NR 18 TC 10 Z9 10 U1 2 U2 11 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 765 EP 770 DI 10.1645/GE-2413.1 PG 6 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800015 PM 20496959 ER PT J AU Hsu, V Grant, DC Dubey, JP Zajac, AM Lindsay, DS AF Hsu, Vasha Grant, David C. Dubey, J. P. Zajac, Anne M. Lindsay, David S. TI Prevalence of Antibodies to Sarcocystis neurona in Cats From Virginia and Pennsylvania SO JOURNAL OF PARASITOLOGY LA English DT Article ID OPOSSUMS DIDELPHIS-VIRGINIANA; RACCOONS PROCYON-LOTOR; PROTOZOAL MYELOENCEPHALITIS EPM; ARMADILLO DASYPUS-NOVEMCINCTUS; DOMESTIC CATS; INTERMEDIATE HOST; FELIS-DOMESTICUS; AGGLUTINATING ANTIBODIES; EXPERIMENTAL INDUCTION; NEUROLOGIC-DISEASE AB Sarcocystis neurone, is best known as the causative agent of equine protozoal myeloencephalitis of horses in the Americas. Domestic cats (Felis domesticus) were the first animals described as an intermediate host for S. neurone,. However, S. neurona-associated encephalitis has also been reported in naturally infected cats in the United States. Thus, cats can be implicated in the life cycle of S. neurona as natural intermediate hosts. The present study examined the seroprevalence of IgG antibodies to merozoites of S. neurona in populations of domestic cats from Virginia and Pennsylvania. Overall, sera or plasma from 441 cats (Virginia = 232, Pennsylvania = 209) were tested by an indirect immunofluorescent assay at a 1:50 dilution. Antibodies to S. neurona were found in 32 (7%) of 441 cats. Of these, 22 (9%) of the 232 cats from Virginia and 10 (5%) of the 209 cats from Pennsylvania were seropositive for S. neurona. C1 [Hsu, Vasha; Zajac, Anne M.; Lindsay, David S.] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. [Grant, David C.] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Small Anim Clin Lab, Blacksburg, VA 24061 USA. [Dubey, J. P.] ARS, Anim Parasit Dis Lab, USDA, Anim & Nat Resources Inst,Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Lindsay, DS (reprint author), Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. EM lindsayd@vt.edu RI Lindsay, David/G-8891-2016 OI Lindsay, David/0000-0002-0592-8321 NR 19 TC 4 Z9 4 U1 2 U2 6 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 800 EP 801 DI 10.1645/GE-2449.1 PG 2 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800021 PM 20476809 ER PT J AU Galaktionov, KV Ryss, AJ Pugachev, O Pelgunov, A Kontrimavichus, VL Hoberg, EP AF Galaktionov, K. V. Ryss, A. J. Pugachev, O. Pelgunov, A. Kontrimavichus, V. L. Hoberg, Eric P. TI MARK DMITRIEVICH SONIN 1932-2010 IN MEMORIAM SO JOURNAL OF PARASITOLOGY LA English DT Biographical-Item C1 [Galaktionov, K. V.; Ryss, A. J.; Pugachev, O.] Russian Acad Sci, Inst Zool, St Petersburg 199034, Russia. [Pelgunov, A.] Russian Acad Sci, Ctr Parasitol, Inst Problems Ecol & Evolut, Moscow 119071, Russia. [Kontrimavichus, V. L.] Ctr Nat Res, Inst Ecol, LT-08412 Vilnius, Lithuania. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Galaktionov, KV (reprint author), Russian Acad Sci, Inst Zool, Univ nab 1, St Petersburg 199034, Russia. RI Pugachev, Oleg/A-3740-2013; Ryss, Alexander/B-9689-2013; Galaktionov, Kirill/D-8874-2012 OI Galaktionov, Kirill/0000-0003-1897-6709 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2010 VL 96 IS 4 BP 836 EP 837 DI 10.1645/GE-2464.1 PG 2 WC Parasitology SC Parasitology GA 645LR UT WOS:000281459800032 ER PT J AU Upchurch, RG Ramirez, ME AF Upchurch, Robert G. Ramirez, Martha E. TI Gene Expression Profiles of Soybeans with Mid-Oleic Acid Seed Phenotype SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE GmFATB1a; GmSACPD-A, -B, -C; GmFAD2-1A,-1B; GmFAD2-2; GmFAD2-3; Allele; Oleic acid; qRT-PCR ID OLEATE CONTENT; DESATURASE ENZYMES; MOLECULAR ANALYSIS; MUTANT M23; LINES; TEMPERATURE; STABILITY; OIL AB Seeds of the mid-oleic acid soybean mutant M23 accumulate higher levels of oleic acid (50-60% oleate) by virtue of a deletion of GmFAD2-1A, an allele of the microsomal omega-6 oleate desaturase gene. In other less well characterized soybean varieties that are phenotypically mid-oleic, little is known about the expression levels of omega-6 desaturase GmFAD2 genes and other candidate genes that determine seed oleic acid content. We compared the steady-state transcript abundance during seed development of the oleate-ACP thioesterase (GmFATB1a), delta-9 stearoyl acyl carrier protein desaturase (GmSACPD) and the omega-6 fatty acid desaturase (GmFAD2) genes in five natural mid-oleic varieties and mutant M23 to gene expression data for the conventional non mid-oleic cultivar Dare. We found that, relative to Dare, there were instances where lower expression of GmFATB1a, GmFAD2-1A, GmFAD2-1B, GmFAD2-2, and GmFAD2-3 and higher expression of the GmSACPD-C might be associated with the mid-oleic seed phenotype. This finding suggests that of the several soybean genomic loci known or suspected to be involved in oleic acid phenotype, some are likely to encode genes involved in regulation of transcription of the oleate biosynthetic genes. C1 [Upchurch, Robert G.; Ramirez, Martha E.] N Carolina State Univ, USDA ARS, Dept Plant Pathol, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27695 USA. RP Upchurch, RG (reprint author), N Carolina State Univ, USDA ARS, Dept Plant Pathol, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27695 USA. EM greg.upchurch@ars.usda.gov NR 29 TC 2 Z9 2 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2010 VL 87 IS 8 BP 857 EP 864 DI 10.1007/s11746-010-1576-z PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 627VL UT WOS:000280071600003 ER PT J AU Moreau, RA Hicks, KB Johnston, DB Laun, NP AF Moreau, Robert A. Hicks, Kevin B. Johnston, David B. Laun, Nathan P. TI The Composition of Crude Corn Oil Recovered after Fermentation via Centrifugation from a Commercial Dry Grind Ethanol Process SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Corn; Zea mays; Carotenoids; Fuel ethanol; Tocopherols; Tocotrienols; Vitamin E; Phytosterols; Plant sterols; Coproducts ID GROUND CORN; EXTRACTION; GERM; FIBER; TOCOMONOENOL; TOCOPHEROL; LIPIDS AB A study was conducted to examine the chemical composition of corn oil obtained after fermentation of corn to make fuel ethanol via centrifugation and compare its composition to that of corn germ oil (commercial corn oil) and experimental corn oils. The levels of free fatty acids in the post fermentation corn oil were high (11-16%), as previously reported. The levels of free phytosterols and hydroxycinnamate steryl esters (similar to oryzanol in rice bran oil) were higher than those of corn germ oil and were comparable to those of ethanol-extracted corn kernel oil. The levels of tocopherols were lower in post-fermentation oil than in either corn germ oil or ethanol extracted corn kernel oil. The levels of lutein and zeaxanthin in post-fermentation were much higher than those in corn germ oil and were comparable to those in ethanol-extracted corn kernel oil. Overall, exposure to all upstream processes of a fuel ethanol plant, including high-temperature liquefaction, saccharification and fermentation appeared to have the most notable effect on tocopherols, but it had little effect on the levels of free phytosterols, hydroxycinnamate steryl esters, lutein and zeaxanthin. It may be desirable to recover these valuable functional lipids prior to using the post-fermentation corn oil for industrial applications such as making biodiesel if a cost-effective recovery process can be developed. C1 [Moreau, Robert A.; Hicks, Kevin B.; Johnston, David B.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Laun, Nathan P.] Western New York Energy LLC, Medina, NY 14103 USA. RP Moreau, RA (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM robert.moreau@ars.usda.gov OI Moreau, Robert/0000-0002-8166-8322 NR 23 TC 16 Z9 17 U1 2 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2010 VL 87 IS 8 BP 895 EP 902 DI 10.1007/s11746-010-1568-z PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 627VL UT WOS:000280071600007 ER PT J AU Thompson, M Sessions, J Boston, K Skaugset, A Tomberlin, D AF Thompson, Matthew Sessions, John Boston, Kevin Skaugset, Arne Tomberlin, David TI Forest Road Erosion Control Using Multiobjective Optimization1 SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE sediment; water policy; optimization; nonpoint source pollution; point source pollution ID OBJECTIVE GENETIC ALGORITHMS; NATURAL-RESOURCE MANAGEMENT; DECISION-ANALYSIS; SEDIMENT PRODUCTION; HARVEST; WILDLIFE; MODEL; DEACTIVATION; WATERSHEDS; STRATEGIES AB Forest roads are associated with accelerated erosion and can be a major source of sediment delivery to streams, which can degrade aquatic habitat. Controlling road-related erosion therefore remains an important issue for forest stewardship. Managers are faced with the task to develop efficient road management strategies to achieve conflicting environmental and economic goals. This manuscript uses mathematical programming techniques to identify the efficient frontier between sediment reduction and treatment costs. Information on the nature of the tradeoffs between conflicting objectives can give the decision maker more insight into the problem, and help in reaching a suitable compromise solution. This approach avoids difficulties associated with a priori establishment of targets for sediment reduction, preferences between competing objectives, and mechanisms to scale noncommensurate objectives. Computational results demonstrate the utility of this multiobjective optimization approach, which should facilitate tradeoff analysis and ideally promote efficient erosion control on forest roads. C1 [Thompson, Matthew] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Sessions, John; Boston, Kevin; Skaugset, Arne] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Tomberlin, David] Natl Marine Fisheries Serv, Silver Spring, MD 20910 USA. RP Thompson, M (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, POB 7669, Missoula, MT 59801 USA. EM mpthompson02@fs.fed.us NR 71 TC 6 Z9 6 U1 4 U2 6 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1093-474X J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2010 VL 46 IS 4 BP 712 EP 723 DI 10.1111/j.1752-1688.2010.00443.x PG 12 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 631ON UT WOS:000280357300006 ER PT J AU Merten, EC Hemstad, NA Kolka, RK Newman, RM Verry, ES Vondracek, B AF Merten, Eric C. Hemstad, Nathaniel A. Kolka, Randall K. Newman, Raymond M. Verry, Elon S. Vondracek, Bruce TI Recovery of Sediment Characteristics in Moraine, Headwater Streams of Northern Minnesota After Forest Harvest1 SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE environmental impacts; riparian ecology; forests; headwaters; rivers; streams; sediment; erosion; watershed management ID BREEDING BIRD RESPONSE; PACIFIC-NORTHWEST; MANAGEMENT ZONES; RIPARIAN BUFFERS; DECIDUOUS FOREST; WASHINGTON; MORTALITY; HABITAT; RUNOFF; CANADA AB We investigated the recovery of sediment characteristics in four moraine, headwater streams in north-central Minnesota after forest harvest. We examined changes in fine sediment levels from 1997 (preharvest) to 2007 (10 years postharvest) at study plots with upland clear felling and riparian thinning, using canopy cover, proportion of unstable banks, surficial fine substrates, residual pool depth, and streambed depth of refusal as response variables. Basin-scale year effects were significant (p < 0.001) for all responses when evaluated by repeated-measures ANOVAs. Throughout the study area, unstable banks increased for several years postharvest, coinciding with an increase in windthrow and fine sediment. Increased unstable banks may have been caused by forest harvest equipment, increased windthrow and exposure of rootwads, or increased discharge and bank scour. Fine sediment in the channels did not recover by summer 2007, even though canopy cover and unstable banks had returned to 1997 levels. After several storm events in fall 2007, 10 years after the initial sediment input, fine sediment was flushed from the channels and returned to 1997 levels. Although our study design did not discern the source of the initial sediment inputs (e.g., forest harvest, road crossings, other natural causes), we have shown that moraine, headwater streams can require an extended period (up to 10 years) and enabling event (e.g., high storm flows) to recover from large inputs of fine sediment. C1 [Merten, Eric C.] Univ Wisconsin, Dept Biol, Eau Claire, WI 54702 USA. [Hemstad, Nathaniel A.] Inver Hills Community Coll, Dept Biol, Inver Grove Hts, MN 55076 USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Newman, Raymond M.] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA. [Verry, Elon S.] Ellen River Partners Inc, Grand Rapids, MN 55744 USA. [Vondracek, Bruce] Univ Minnesota, USGS, Minnesota Cooperat Fish & Wildlife Res Unit, St Paul, MN 55108 USA. RP Merten, EC (reprint author), Univ Wisconsin, Dept Biol, 105 Garfield Ave, Eau Claire, WI 54702 USA. EM mertenec@uwec.edu FU Minnesota Department of Natural Resources Division of Fisheries; Minnesota Forest Resources Council; National Council for Air and Stream Improvement; U.S. Forest Service; Minnesota Environment and Natural Resources Trust Fund; Minnesota Trout Unlimited; U.S. Geological Survey; University of Minnesota; Minnesota Department of Natural Resources; Wildlife Management Institute FX This work was funded by the Minnesota Department of Natural Resources Division of Fisheries, the Minnesota Forest Resources Council, the National Council for Air and Stream Improvement, the U.S. Forest Service, the Minnesota Environment and Natural Resources Trust Fund, and Minnesota Trout Unlimited. Charlie Blinn and Brian Palik selected the study plots and supervised the manipulations. John Hansen and Jim Marshall of UPM-Kymenne Corporation Blandin provided access to the study plots. Forest harvest was completed by Rieger Logging. We sincerely thank the following for assistance with data collection: Andy Arola, Brenda Asmus, Jason Bronk, Rebecca Bronk, Ryan Carlson, Bill Coates, Jacquelyn Conner, Carrie Dorrance, Art Elling, MaryKay Fox, Jo Fritz, Sarah Harnden, Deacon Kyllander, Marty Melchior, Steffen Merten, Mateya Miltich, Brittany Mitchell, Erik Mundahl, Elliot Nitzkowski, Ian Phelps, Lisa Pugh, Jeff Rice, David Schroeder, Jeremy Steil, Dustin Wilman, and Jason Zwonitzer. The Statistical Consulting Service at the University of Minnesota provided R code for analysis. Comments from Jacques Finlay, Heinz Stefan, and three anonymous reviewers improved the quality of the manuscript. The Minnesota Cooperative Fish and Wildlife Research Unit is jointly sponsored by the U.S. Geological Survey, the University of Minnesota, the Minnesota Department of Natural Resources, and the Wildlife Management Institute. NR 45 TC 2 Z9 2 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2010 VL 46 IS 4 BP 733 EP 743 DI 10.1111/j.1752-1688.2010.00445.x PG 11 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 631ON UT WOS:000280357300008 ER PT J AU Lee, BH Kim, WH Jeong, J Yoo, J Kwon, YK Jung, BY Kwon, JH Lillehoj, HS Min, W AF Lee, Byung H. Kim, Woo H. Jeong, Jipseol Yoo, Jeongmi Kwon, Yong-Kuk Jung, Byeong Y. Kwon, Jun H. Lillehoj, Hyun S. Min, Wongi TI Prevalence and Cross-Immunity of Eimeria Species on Korean Chicken Farms SO JOURNAL OF VETERINARY MEDICAL SCIENCE LA English DT Article DE chickens; cross-protection; Eimeria; Korea; prevalence ID MAXIMA; COCCIDIOSIS; BROILERS; IDENTIFICATION; PROTECTION; ANTIBODIES; STRAINS; LITTER; DRUGS AB Epidemiology of Eimeria species in poultry flocks is important to increase the effectiveness of vaccinations and prophylactic strategies on chicken farms. In this study, fecal samples from 356 chicken farms were collected randomly and examined for the prevalence of Eimeria species. Through microscopic examination, it was determined that 78.7% of the tested farms were positive in Eimeria-infection. Seven Eimeria species were detected in all the positive farms by PCR amplification or the internal transcribed spacer 1 (ITS-1) region with species-specific primers. E. acervulina and E. tenella were the most prevalent, followed by E. brunetti and E. praecox (87.5, 62.5, 59.3, and 37.5% of the farms, respectively). Each of E. maxima, E. mitis, and E. necatrix was identified in 31.3% of the farms. Individual positive fecal samples contained multiple Eimeria species (mean=3.4). Since E. maxima is known to generate antigenic variants, cross-immunity was investigated for four isolates of E. maxima from the poultry farms in different regions of Korea. The extent of cross-protection varied from 54.3 to 100% against the heterologous isolates. The results obtained from this large-scale survey will be a useful reference for controlling coccidiosis in the poultry industry. C1 [Lee, Byung H.; Kim, Woo H.; Jeong, Jipseol; Yoo, Jeongmi; Min, Wongi] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea. [Lee, Byung H.; Kim, Woo H.; Jeong, Jipseol; Yoo, Jeongmi; Min, Wongi] Gyeongsang Natl Univ, Life Sci Res Inst, Jinju 660701, Gyeongnam, South Korea. [Kwon, Yong-Kuk; Jung, Byeong Y.; Kwon, Jun H.] Minist Food Agr Forestry & Fisheries, Natl Vet Res & Quarantine Serv, Anyang 430824, South Korea. [Lillehoj, Hyun S.] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. RP Min, W (reprint author), Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea. EM wongimin@gnu.ac.kr OI Min, Wongi/0000-0003-2437-7366 FU National Veterinary Research & Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Korea [Z-AD15-2008-09-01] FX This study was supported by a 2008 grant (Z-AD15-2008-09-01) from National Veterinary Research & Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Korea. NR 25 TC 19 Z9 20 U1 0 U2 3 PU JAPAN SOC VET SCI PI TOKYO PA UNIV TOKYO, 1-1-1 YAYOI, BUNKYO-KU, TOKYO, 103, JAPAN SN 0916-7250 J9 J VET MED SCI JI J. Vet. Med. Sci. PD AUG PY 2010 VL 72 IS 8 BP 985 EP 989 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 659EO UT WOS:000282550000004 PM 20234110 ER PT J AU Grisham, MP Johnson, RM Zimba, PV AF Grisham, Michael P. Johnson, Richard M. Zimba, Paul V. TI Detecting Sugarcane yellow leaf virus infection in asymptomatic leaves with hyperspectral remote sensing and associated leaf pigment changes SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Sugarcane; Sugarcane yellow leaf virus; Remote sensing; Hyperspectral; Leaf pigments ID HAND-HELD RADIOMETRY; SPECTRAL REFLECTANCE; MELANAPHIS-SACCHARI; PLANT-LEAVES; DISEASE; LOUISIANA; IMAGERY; YIELD AB Sugarcane infected with Sugarcane yellow leaf virus (SCYLV) rarely produces visual symptoms until late in the growing season High-resolution, hyperspectral reflectance data from SCYLV-infected and noninfected leaves of two cultivars, LCP 85-384 and Ho 95-988, were measured and analyzed on 13 July, 12 October, and 4 November 2005 All plants were asymptomatic Infection was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis Results from discriminant analysis showed that leaf reflectance was effective at predicting SCYLV infection in 73% of the cases in both cultivars using resubstitution and 63% and 62% in LCP 85-384 and Ho 95-988, respectively, using cross-validation Predictive equations were improved when data from sampling dates were analyzed individually SCYLV infection influenced the concentration of several leaf pigments including violaxanthin, beta-carotene, neoxanthin. and chlorophyll a Pigment data were effective at predicting SCYLV infection in 80% of the samples in the combined data set using the derived discriminant function with resubstitution, and 71% with cross-validation Although further research is needed to improve the accuracy of the predictive equations, the results of this study demonstrate the potential application of hyperspectral remote sensing as a rapid, field-based method of identifying SCYLV-infected sugarcane plants prior to symptom expression Published by Elsevier B V C1 [Grisham, Michael P.; Johnson, Richard M.] USDA ARS, Sugarcane Res Lab, Houma, LA 70360 USA. [Zimba, Paul V.] Texas A&M Univ, Ctr Coastal Studies, Corpus Christi, TX 78412 USA. RP Grisham, MP (reprint author), USDA ARS, Sugarcane Res Lab, 5883 USDA Rd, Houma, LA 70360 USA. RI Zimba, Paul/O-2778-2013 NR 41 TC 10 Z9 14 U1 0 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 J9 J VIROL METHODS JI J. Virol. Methods PD AUG PY 2010 VL 167 IS 2 BP 140 EP 145 DI 10.1016/j.jviromet.2010.03.024 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA 621HT UT WOS:000279567400005 PM 20362003 ER PT J AU Cilloniz, C Pantin-Jackwood, MJ Ni, C Goodman, AG Peng, XX Proll, SC Carter, VS Rosenzweig, ER Szretter, KJ Katz, JM Korth, MJ Swayne, DE Tumpey, TM Katze, MG AF Cilloniz, Cristian Pantin-Jackwood, Mary J. Ni, Chester Goodman, Alan G. Peng, Xinxia Proll, Sean C. Carter, Victoria S. Rosenzweig, Elizabeth R. Szretter, Kristy J. Katz, Jacqueline M. Korth, Marcus J. Swayne, David E. Tumpey, Terrence M. Katze, Michael G. TI Lethal Dissemination of H5N1 Influenza Virus Is Associated with Dysregulation of Inflammation and Lipoxin Signaling in a Mouse Model of Infection SO JOURNAL OF VIROLOGY LA English DT Article ID ASPIRIN-TRIGGERED LIPOXIN; EBOLA HEMORRHAGIC-FEVER; A H1N1 VIRUS; ANTIVIRAL DEFENSE; IMMUNE-RESPONSE; MICE; ORIGIN; DISEASE; TRANSMISSION; INTERFERONS AB Periodic outbreaks of highly pathogenic avian H5N1 influenza viruses and the current H1N1 pandemic highlight the need for a more detailed understanding of influenza virus pathogenesis. To investigate the host transcriptional response induced by pathogenic influenza viruses, we used a functional-genomics approach to compare gene expression profiles in lungs from 129S6/SvEv mice infected with either the fully reconstructed H1N1 1918 pandemic virus (1918) or the highly pathogenic avian H5N1 virus Vietnam/1203/04 (VN/1203). Although the viruses reached similar titers in the lung and caused lethal infections, the mean time of death was 6 days for VN/1203-infected animals and 9 days for mice infected with the 1918 virus. VN/1203-infected animals also exhibited an earlier and more potent inflammatory response. This response included induction of genes encoding components of the inflammasome. VN/1203 was also able to disseminate to multiple organs, including the brain, which correlated with changes in the expression of genes associated with hematological functions and lipoxin biogenesis and signaling. Both viruses elicited expression of type I interferon (IFN)-regulated genes in wild-type mice and to a lesser extent in mice lacking the type I IFN receptor, suggesting alternative or redundant pathways for IFN signaling. Our findings suggest that VN/1203 is more pathogenic in mice as a consequence of several factors, including the early and sustained induction of the inflammatory response, the additive or synergistic effects of upregulated components of the immune response, and inhibition of lipoxin-mediated anti-inflammatory responses, which correlated with the ability of VN/1203 to disseminate to extrapulmonary organs. C1 [Cilloniz, Cristian; Ni, Chester; Goodman, Alan G.; Peng, Xinxia; Proll, Sean C.; Carter, Victoria S.; Rosenzweig, Elizabeth R.; Korth, Marcus J.; Katze, Michael G.] Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA. [Pantin-Jackwood, Mary J.; Swayne, David E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30606 USA. [Szretter, Kristy J.] Washington Univ, Dept Med, St Louis, MO 63110 USA. [Katz, Jacqueline M.; Tumpey, Terrence M.] Ctr Dis Control & Prevent, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. [Katze, Michael G.] Univ Washington, Washington Natl Primate Res Ctr, Seattle, WA 98195 USA. RP Katze, MG (reprint author), Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA. EM honey@u.washington.edu FU National Institute of Allergy and Infectious Diseases [P01 AI058113, 2P01 AI058113]; Current Research Information Systems Project [6612-32000-049-00D] FX This study was funded in part by National Institute of Allergy and Infectious Diseases grants P01 AI058113 and 2P01 AI058113 (to M.G.K.) and Current Research Information Systems Project 6612-32000-049-00D (to D.E.S.). NR 46 TC 58 Z9 59 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 2010 VL 84 IS 15 BP 7613 EP 7624 DI 10.1128/JVI.00553-10 PG 12 WC Virology SC Virology GA 626TK UT WOS:000279989800018 PM 20504916 ER PT J AU Cao, MX Ye, XH Willie, K Lin, JY Zhang, XC Redinbaugh, MG Simon, AE Morris, TJ Qu, F AF Cao, Mingxia Ye, Xiaohong Willie, Kristen Lin, Junyan Zhang, Xiuchun Redinbaugh, Margaret G. Simon, Anne E. Morris, T. Jack Qu, Feng TI The Capsid Protein of Turnip Crinkle Virus Overcomes Two Separate Defense Barriers To Facilitate Systemic Movement of the Virus in Arabidopsis SO JOURNAL OF VIROLOGY LA English DT Article ID BUSHY-STUNT-VIRUS; DICER-LIKE PROTEINS; COAT PROTEIN; NICOTIANA-BENTHAMIANA; GENE; SUPPRESSION; RESISTANCE; PLANTS; RNAS; P19 AB The capsid protein (CP) of Turnip crinkle virus (TCV) is a multifunctional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long-distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV CP and evaluated the interdependence of these functions. A series of TCV mutants containing alterations in the CP coding region were generated. These alterations range from single-amino-acid substitutions and domain truncations to knockouts of CP translation. The latter category also contained two constructs in which the CP coding region was replaced by either the cDNA of a silencing suppressor of a different virus or that of green fluorescent protein. These mutants were used to infect Arabidopsis plants with diminished antiviral silencing capability (dcl2 dcl3 dcl4 plants). There was a strong correlation between the ability of mutants to reach systemic leaves and the silencing suppressor activity of mutant CP. Virus particles were not essential for entry of the viral genome into vascular bundles in the inoculated leaves in the absence of antiviral silencing. However, virus particles were necessary for egress of the viral genome from the vasculature of systemic leaves. Our experiments demonstrate that TCV CP not only allows the viral genome to access the systemic movement channel through silencing suppression but also ensures its smooth egress by way of assembled virus particles. These results illustrate that efficient long-distance movement of TCV requires both functions afforded by the CP. C1 [Cao, Mingxia; Lin, Junyan; Zhang, Xiuchun; Qu, Feng] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Ye, Xiaohong; Morris, T. Jack] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68583 USA. [Willie, Kristen; Redinbaugh, Margaret G.] USDA ARS, Corn & Soybean Virus Unit, Wooster, OH 44691 USA. [Simon, Anne E.] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. RP Qu, F (reprint author), 024 Selby Hall,1680 Madison Ave, Wooster, OH 44691 USA. EM qu.28@osu.edu RI Redinbaugh, Margaret/A-3611-2013 FU OARDC; Ohio Plant Biotechnology Consortium FX This study is supported by seed grants from OARDC and the Ohio Plant Biotechnology Consortium. NR 33 TC 34 Z9 34 U1 1 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 2010 VL 84 IS 15 BP 7793 EP 7802 DI 10.1128/JVI.02643-09 PG 10 WC Virology SC Virology GA 626TK UT WOS:000279989800037 PM 20504923 ER PT J AU Ruggiero, LF AF Ruggiero, Leonard F. TI Scientific Independence and Credibility in Sociopolitical Processes SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE conservation; independence; public policy; reliability; scientific credibility; sociopolitical processes ID CONSERVATION-BIOLOGY; POLICY ADVOCACY; SCIENCE; MANAGEMENT AB Absence of scientific independence can be associated with a lack of impartiality and therefore with a lack of credibility. Yet scientific credibility is essential for effective participation in sociopolitical processes-processes that necessarily involve politics and often result in decisions about land management, conservation, and public policy. All scientists are aware of these processes, many wish to participate, and some wish to advocate for their personal policy preferences. However, scientists who lack impartiality often create the perception of bias, and they can suffer a concomitant loss of credibility. Some policy-makers also have personal preferences for certain policies, and the term normative policies can be used here even though all policies can be viewed as normative in the sense that they involve multiple inputs. Hence, the idea that scientists must provide unbiased information for unbiased application by policy-makers is sometimes wrong. For scientists to be effective participants in sociopolitical processes that lead to conservation policies or related actions, they should inform the public about issues while avoiding direct involvement in policy development and the political considerations this necessarily entails. Scientists should only participate in the decision-making process with impartial information and in their proper role as objective scientists. C1 Forest Serv Res, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Ruggiero, LF (reprint author), Forest Serv Res, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. EM lruggiero@fs.fed.us FU Rocky Mountain Research Station, a major division of the Research and Development Branch of the United States Department of Agriculture Forest Service FX This work was supported by the Rocky Mountain Research Station, a major division of the Research and Development Branch of the United States Department of Agriculture Forest Service. I thank W. Block, J. Canton-Thompson, S. Cushman, K. McKelvey, D. Pearson, S. Ritter, J. Saveland, M. Schwartz, J. Squires, and 2 anonymous reviewers for helpful comments on this manuscript and for discussion of relevant topics. Ideas presented here do not necessarily reflect those of any organization. NR 24 TC 7 Z9 7 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2010 VL 74 IS 6 BP 1179 EP 1182 DI 10.2193/2009-437 PG 4 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 630ZT UT WOS:000280316700002 ER PT J AU Grubb, TG Delaney, DK Bowerman, WW Wierda, MR AF Grubb, Teryl G. Delaney, David K. Bowerman, William W. Wierda, Michael R. TI Golden Eagle Indifference to Heli-Skiing and Military Helicopters in Northern Utah SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE aircraft; buffers; golden eagle; helicopter; heli-skiing; human disturbance; management; military; noise; recreation ID LEVEL JET AIRCRAFT; HUMAN DISTURBANCE; BALD EAGLES; RESPONSES; RAPTORS; NOISE; RECOMMENDATIONS; BEHAVIOR AB In 2006-2007, during Wasatch Powderbird Guides (WPG) permit renewal for heli-skiing in the Tri-Canyon Area (TCA) of the Wasatch Mountains, Utah, USA, we recorded 303 helicopter passes between 0 m and 3,000 m (horizontal distance) near >= 30 individual golden eagles (Aquila chrysaetos) in 22 nesting territories, through passive observation and active experimentation with civilian and military (Apache AH-64) helicopters. Flight profiles included 800-m, 400-m, 200-m, and 100-m flybys (horizontal distance from cliff nest on parallel course), as well as approaches and popouts where helicopters flew toward, or popped out from behind, adult-occupied cliff nests (0 m, horizontal distance). Between 1981 and 2007, during the only 8 years when nesting in the TCA was confirmed by presence of chicks, WPG annually flew 108-2,836 helicopter flights in the same drainages on 10-37 days between 15 December and 15 April, with no effect on early courtship, nest repair, or subsequent nesting success. Total WPG operating days (x = 62.4) and helicopter hours (x = 210.6) fluctuated annually but did not increase 1974-2007 (Cox-Stuart trend test, P = 0.371, 0.393, respectively). Apache helicopter testing (227 passes) did not reduce golden eagle nesting success or productivity rates within the same year (t(111,) (96) = 0.495, 0.782, P = 0.622, 0.436, respectively), or rates of renewed nesting activity the following year, compared with 81-101 non-manipulated nesting territories. We recorded no response during 66% and only watching during 30% of Apache passes at 0-800 m from nesting golden eagles. No other reactions occurred until after hatching when <= 4 golden eagles accounted for 5 flatten and 3 fly behaviors at 3 nest sites. No responding pairs failed to fledge young because of testing. Limited fly responses suggested helicopters only precipitated an imminent departure, rather than causing startled, avoidance reactions. Responsiveness between test weeks 1 and 2 decreased (chi(2)(2) - 32.167, P <= 0.001). Apache helicopters were twice as loud as WPG helicopters at comparable distances. Sound decreased with distance, most rapidly when flights were perpendicular to cliffs or ridges. Eagle ambient behaviors and watching the helicopter occurred randomly throughout recorded sound levels during helicopter testing (76.7-108.8 decibels, unweighted). Much helicopter sound energy is below golden eagles' auditory threshold, thus reducing potential impacts. Neither our observations nor our testing indicated special management restrictions are required for helicopters flying near nesting golden eagles in northern Utah. Our results underscore the necessity for circumstance-specific research, as well as enlightened resource management to accommodate unexpected results. C1 [Grubb, Teryl G.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Delaney, David K.] USA, Engineer Res & Dev Ctr, Construct Engn Res Lab, Champaign, IL 61822 USA. [Bowerman, William W.; Wierda, Michael R.] Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29634 USA. RP Grubb, TG (reprint author), US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM tgrubb@fs.fed.us RI Jenkins, Stephanie/F-5216-2011 FU USFS; Rocky Mountain Research Station; WCNF; United States Army Engineer Research and Development Center, Construction Engineering Research Laboratory; Department of Forestry and Natural Resources, Clemson University FX This research project was jointly funded by the USFS, Rocky Mountain Research Station and WCNF; United States Army Engineer Research and Development Center, Construction Engineering Research Laboratory; and Department of Forestry and Natural Resources, Clemson University. In addition, the WCNF Supervisor's Office and Salt Lake Ranger District, especially D. Probasco, R. Williams, S. Scheid, and J. Walkes, provided logistical and administrative support throughout the project. Helicopter testing was made possible by the Utah National Guard and the special assistance of Senior Pilots CW5 D. Jacobson and CW3 M. Pluim. R. Dassing, M. Olson, and K. O'Rourke of WPG provided historical insight, access to operational records, and helicopter support for several surveys and experimental test flights. Classic Helicopters Head Pilot, M. Nicolette, and Cirque Lodge's Pilot, A. Oprie, arranged for sound testing of their respective helicopters, plus the Cirque helicopter facilitated experimental data collection at 2 additional sites. K. Keller, local raptor specialist and golden eagle expert, provided current and historical nesting data, and monitored manipulated and non-manipulated sites each year. Individuals who recorded helicopter test observations at golden eagle nests include A. Gatto, K. Hartman, D. Johnson, K. Keller, R. Lopez, B. Piscapo, D. Probasco, S. Scheid, C. Smith, Z. Todd, and R. Williams. We thank D. Ellis, D. Garcelon, and M. Kochert for constructively reviewing this manuscript. NR 46 TC 3 Z9 3 U1 3 U2 20 PU WILDLIFE SOC PI BETHESDA PA 5410 GROSVENOR LANE, BETHESDA, MD 20814-2197 USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2010 VL 74 IS 6 BP 1275 EP 1285 DI 10.2193/2009-337 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 630ZT UT WOS:000280316700013 ER PT J AU Matthews, CE Moorman, CE Greenberg, CH Waldrop, TA AF Matthews, Charlotte E. Moorman, Christopher E. Greenberg, Cathryn H. Waldrop, Thomas A. TI Response of Reptiles and Amphibians to Repeated Fuel Reduction Treatments SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE amphibians; fire surrogates; forest management; fuel reduction; herpetofauna; prescribed fire; reptiles; salamanders; southern Appalachian Mountains ID SOUTHERN APPALACHIAN SALAMANDERS; UPLAND HARDWOOD FOREST; BLUE RIDGE MOUNTAINS; PLETHODONTID SALAMANDERS; TERRESTRIAL SALAMANDERS; PRESCRIBED FIRE; SMALL MAMMALS; OAK FORESTS; DISTURBANCE; ABUNDANCE AB Recent use of prescribed fire and fire surrogates to reduce fuel hazards has spurred interest in their effects on wildlife. Studies of fire in the southern Appalachian Mountains (USA) have documented few effects on reptiles and amphibians. However, these studies were conducted after only one fire and for only a short time (1-3 yr) after the fire. From mid-May to mid-August 2006 and 2007, we used drift fences with pitfall and funnel traps to capture reptiles and amphibians in a control and 3 replicated fuel-reduction treatments: 1) twice-burned (2003 and 2006), 2) mechanical understory cut (2002), and 3) mechanical understory cut (2002) followed by 2 burns (2003 and 2006). We captured fewer salamanders in mechanical + twice-burned treatment areas than in twice-burned and control treatment areas, but we captured more lizards in mechanical + twice-burned treatment areas than in other treatment areas. Higher lizard captures in mechanical + twice-burned treatment areas likely was related to increased ground temperatures and greater thermoregulatory opportunities. Higher and more variable ground temperatures and faster drying of remaining litter and duff may have led to fewer salamander captures in mechanical + twice-burned treatment areas. Our longer term results, after 2 prescribed burns, differ from shorter term results. After one prescribed burn at the same site, eastern fence lizard (Sceloporus undulatus) captures were greater in mechanical + burn treatment areas but salamander captures did not differ among treatment areas. Our results indicate that multiple (>= 2) fuel-reduction treatments that decrease canopy cover may benefit lizards but negatively affect salamanders. C1 [Matthews, Charlotte E.; Moorman, Christopher E.] N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries & Wildlife Sci Program, Raleigh, NC 27695 USA. [Greenberg, Cathryn H.] US Forest Serv, USDA, So Res Stn, Asheville, NC 28806 USA. [Waldrop, Thomas A.] US Forest Serv, USDA, So Res Stn, Clemson, SC 29634 USA. RP Matthews, CE (reprint author), Wyoming Game & Fish Dept, 3030 Energy Lane, Casper, WY 82604 USA. EM charematthews@yahoo.com FU United States Joint Fire Science Program; United States Forest Service, Southern Research Station through the National Fire Plan [SRS-4156]; North Carolina State University Department of Forestry and Environmental Resources FX This is Contribution Number 192 of the National Fire and Fire Surrogate Project, funded by the United States Joint Fire Science Program, the United States Forest Service, Southern Research Station (SRS-4156) through the National Fire Plan, and the North Carolina State University Department of Forestry and Environmental Resources. A United States Forest Service team, consisting of R. Phillips, H. Mohr, G. Chapman, C. Flint, and M. Smith assisted in the field, and R. Phillips collected habitat, fuel, and fire data. R. Phillips contributed the map for this manuscript. K. Pollock provided advice on experimental design and statistical analyses, and J. Smith assisted with statistical analyses. We thank R. Medford, S. Mickletz, and V. Montrone for assistance in establishing and maintaining traps and for sampling reptiles, amphibians, and vegetation. D. Simon and the North Carolina Wildlife Resources Commission supervised treatments. S. Bosworth, D. Cooper, G. Graeter, K. Frick, A. Matthews, D. Matthews, R. Matthews, M. Sandfoss, A. Savage, C. Shake, and R. Swiers helped with field work. C. Deperno provided some supplies. S. Hutchens provided some supplies and comments on an earlier draft of the manuscript. NR 46 TC 21 Z9 22 U1 8 U2 30 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2010 VL 74 IS 6 BP 1301 EP 1310 DI 10.2193/2008-513 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 630ZT UT WOS:000280316700016 ER PT J AU Werner, SJ Linz, GM Tupper, SK Carlson, JC AF Werner, Scott J. Linz, George M. Tupper, Shelagh K. Carlson, James C. TI Laboratory Efficacy of Chemical Repellents for Reducing Blackbird Damage in Rice and Sunflower Crops SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Agelaius phoeniceus; carboxin; chemical repellent; chlorpyrifos; gamma-cyhalothrin; metalaxyl; red-winged blackbird; thiram; trifloxystrobin; wildlife damage management ID REGISTERED PESTICIDES; CAFFEINE AB Nonlethal alternatives are needed to manage blackbird (Icterids) damage to rice and sunflower production in the United States. We evaluated 4 registered fungicides on rice seeds (i.e., Allegiance (R) FL, Thiram 42-S, Trilex (R), and Vitavax (R) 200 preplant seed treatments) and 2 foliar pesticides on sunflower seeds (Cobalt (TM) insecticide and Flock Buster bird repellent) as candidate blackbird repellents. Red-winged blackbirds (Agelaius phoeniceus) preferred untreated rice relative to rice treated with Thiram (P < 0.001) and Vitavax (P < 0.001), and untreated sunflower relative to sunflower treated with Cobalt (P < 0.001). Blackbirds preferred untreated sunflower relative to sunflower treated with Flock Buster repellent on day 1 of a 4-day preference test (P < 0.001). We observed no difference in consumption of treated versus untreated rice during the Allegiance preference test (P = 0.928), and blackbirds preferred rice treated with Trilex relative to untreated rice (P = 0.003). Although repellency was positively related to tested concentrations of Thiram (P = 0.010), Trilex (P = 0.026), and Vitavax (P, 0.001), maximum repellency was <50% during our concentration-response tests of these seed treatments. Repellency was also positively related to tested concentrations of Cobalt (P < 0.001), and we observed > 80% repellency of sunflower treated with Cobalt at >= 50% of the label rate. We observed no concentration-response relationship for the Allegiance seed treatment (P = 0.341) and Flock Buster repellent (P = 0.952). We recommend implementation of supplemental field studies to compare laboratory efficacy, repellency, and chemical residues of effective avian repellents throughout periods of needed crop protection. C1 [Werner, Scott J.; Linz, George M.; Tupper, Shelagh K.; Carlson, James C.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Werner, SJ (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Scott.J.Werner@aphis.usda.gov OI Tupper, Shelagh/0000-0002-9936-487X FU Louisiana Rice Research Board; Louisiana State University Rice Research Station; Rice Foundation; U.S.A. Rice Federation; National Sunflower Association; United States Department of Agriculture FX This research was supported by the Louisiana Rice Research Board, the Louisiana State University Rice Research Station, the Rice Foundation, the U.S.A. Rice Federation, and the National Sunflower Association. Our rice tests were conducted with Bayer Crop Science seed treatments. Our sunflower tests were conducted with Cobalt insecticide (Dow AgroSciences) and Flock Buster repellent (Skeet-R-Gone). Corporate collaborations do not imply endorsement by the United States Department of Agriculture. We appreciate the NWRC animal care staff that provided daily care throughout our feeding tests. We also thank J. Eisemann, S. Pettit, and B. Washburn for constructive feedback from their review of our manuscript. NR 20 TC 15 Z9 15 U1 1 U2 12 PU WILDLIFE SOC PI BETHESDA PA 5410 GROSVENOR LANE, BETHESDA, MD 20814-2197 USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2010 VL 74 IS 6 BP 1400 EP 1404 DI 10.2193/2009-287 PG 5 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 630ZT UT WOS:000280316700029 ER PT J AU Goslee, SC Sanderson, MA AF Goslee, Sarah C. Sanderson, Matt A. TI Landscape context and plant community composition in grazed agricultural systems of the Northeastern United States SO LANDSCAPE ECOLOGY LA English DT Article DE Agriculture; Climate; Diversity; Grazing; Land use; Landscape pattern; Soils; Species richness; Topography ID SPECIES RICHNESS; SEMINATURAL PASTURES; RURAL LANDSCAPES; LAND-COVER; GRASSLANDS; BIODIVERSITY; MANAGEMENT; PATTERNS; HABITAT; PATCH AB Temperate humid grazing lands are an important component of the landscape of the northeastern United States, as well as of the economy of this region. Unlike their European counterparts, little is known about the basic ecology of managed grasslands in this region. During an 8-year survey of 28 farms across the northeastern United States, we sampled the vegetation on 95 grazed plots, identifying 310 plant species, and collected data on topography, climate and soils. Landscape structure data were obtained from the National Land Cover Data (NLCD) 2001 for six radii (250-2,000 m) surrounding each site. The 500-m radius was most strongly related to plant community composition. Planned species composition was related only to site factors, while associated species were influenced by both site factors and landscape pattern. Species richness was unrelated to landscape structure for either group. Differing management effects on planned and associated species may explain the variation in their responses. Managed grasslands are a critical part of the interconnected landscape of the northeastern United States, and both affect and are affected by their surroundings. C1 [Goslee, Sarah C.; Sanderson, Matt A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16801 USA. RP Goslee, SC (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16801 USA. EM sarah.goslee@ars.usda.gov RI Wrbka, Thomas/A-5699-2010 OI Wrbka, Thomas/0000-0002-1451-9108 FU United States Department of Agriculture's Natural Resources Conservation Service; Agricultural Research Service; Cooperative State Research, Education, and Extension Service FX B. F. Tracy was responsible for establishing this project and much of the early data collection. J. M. Gonet participated in vegetation sampling and geographic data analysis. This work contributes to the Conservation Effects Assessment Project (CEAP), jointly funded, coordinated and administered by the United States Department of Agriculture's Natural Resources Conservation Service, Agricultural Research Service, and Cooperative State Research, Education, and Extension Service. NR 37 TC 8 Z9 8 U1 2 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2010 VL 25 IS 7 BP 1029 EP 1039 DI 10.1007/s10980-010-9477-y PG 11 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA 621PC UT WOS:000279592100004 ER PT J AU Kim, JH Campbell, BC Mahoney, N Chan, KL Molyneux, RJ Xiao, CL AF Kim, J. H. Campbell, B. C. Mahoney, N. Chan, K. L. Molyneux, R. J. Xiao, C. L. TI Use of chemosensitization to overcome fludioxonil resistance in Penicillium expansum SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE filamentous fungi; MAPK signalling; natural products; oxidative stress; patulin ID 2-COMPONENT HISTIDINE KINASE; ACTIVATED PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-FUMIGATUS; SIGNAL-TRANSDUCTION; ANTIFUNGAL ACTIVITY; CANDIDA-ALBICANS; STRESS-RESPONSE; COMPLEX-III; FLUCONAZOLE AB Aim: To overcome fludioxonil resistance of Penicillium expansum, a mycotoxigenic fungal pathogen causing postharvest decay in apple, by using natural phenolic chemosensitizing agents. Methods and Results: Fludioxonil-resistant mutants of P. expansum were co-treated with different oxidising and natural phenolic agents. Resistance was overcome by natural phenolic chemosensitizing agents targeting the oxidative stress-response pathway. These agents also augmented effectiveness of the fungicide, kresoxim-methyl. Results indicated that alkyl gallates target mitochondrial respiration and/or its antioxidation system. Fungal mitochondrial superoxide dismutase (Mn-SOD) plays a protective role against alkyl gallates. Conclusions: Natural chemosensitizing agents targeting the oxidative stress-response system, such as Mn-SOD, can synergize commercial fungicides. Significance and Impact of the Study: Redox-active compounds can serve as potent chemosensitizing agents to overcome resistance and lower effective dosages of fungicides. This can reduce costs with coincidental lowering of environmental and health risks. C1 [Kim, J. H.; Campbell, B. C.; Mahoney, N.; Chan, K. L.; Molyneux, R. J.] USDA ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Xiao, C. L.] Washington State Univ, Dept Plant Pathol, Wenatchee, WA USA. RP Campbell, BC (reprint author), USDA ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM bruce.campbell@ars.usda.gov FU USDA [5325-42000-035-00D]; Washington Tree Fruit Research Commission [CP-07-701] FX This research was conducted under USDA-ARS CRIS Project 5325-42000-035-00D and Washington Tree Fruit Research Commission Project no. CP-07-701. NR 36 TC 7 Z9 7 U1 2 U2 10 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0266-8254 J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD AUG PY 2010 VL 51 IS 2 BP 177 EP 183 DI 10.1111/j.1472-765X.2010.02875.x PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 623OB UT WOS:000279750600009 PM 20536709 ER PT J AU Wang, S Wu, DY Matthan, NR Lamon-Fava, S Lecker, JL Lichtenstein, AH AF Wang, Shu Wu, Dayong Matthan, Nirupa R. Lamon-Fava, Stefania Lecker, Jaime L. Lichtenstein, Alice H. TI Enhanced Aortic Macrophage Lipid Accumulation and Inflammatory Response in LDL Receptor Null Mice Fed an Atherogenic Diet SO LIPIDS LA English DT Article DE Atherogenic diet; Atherosclerosis; Cholesterol accumulation; Inflammatory response; Macrophage; LDLr-/- mouse ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; PERIPHERAL ARTERIAL-DISEASE; CASSETTE TRANSPORTER A1; AGE-ASSOCIATED INCREASE; CORONARY-HEART-DISEASE; TOLL-LIKE RECEPTOR-4; LIVER-X-RECEPTOR; FATTY-ACIDS; DENSITY-LIPOPROTEIN; SCAVENGER RECEPTORS AB The effect of an atherogenic diet on inflammatory response and elicited peritoneal macrophage (MI center dot) cholesterol accumulation in relation to aortic lesion formation was assessed in LDL receptor null (LDLr-/-) mice. Mice were fed an atherogenic or control diet for 32 weeks. The atherogenic relative to control diet resulted in significantly higher plasma monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF alpha) and interleukin-6 (IL-6) concentrations, more aortic wall MI center dot deposition, higher serum non HDL-cholesterol concentrations and total cholesterol to HDL-cholesterol ratios, and greater accumulation of both aortic free and esterified cholesterol. Elicited peritoneal MI center dot selectively accumulated longer chain unsaturated fatty acids in their membrane, independent of the dietary fatty acid profile. Elicited peritoneal MI center dot isolated from mice fed the atherogenic relative to control diet had significantly less arachidonic acid levels, accumulated significantly higher esterified cholesterol, had significantly higher mRNA levels and secretion of MCP-1, and mRNA and protein levels of ATP-binding cassette A1. Diet treatment had no significant effect in elicited peritoneal MI center dot on TNF alpha and IL-6 mRNA levels and secretion. These data suggest that the atherogenic relative to control diet resulted in higher plasma inflammatory factor concentrations, less favorable lipoprotein profile, higher elicited peritoneal MI center dot cholesterol accumulation and inflammatory factor secretion, and more aortic wall MI center dot deposition, which in turn were associated with greater aortic cholesterol accumulation. C1 [Wang, Shu; Matthan, Nirupa R.; Lecker, Jaime L.; Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Wu, Dayong] Tufts Univ, Nutr Immunol Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lamon-Fava, Stefania] Tufts Univ, Lipid Metab Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Cardiovasc Nutr Lab, JM USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu FU USDA [588-1950-9-001]; [T32 HL69772-01A1]; [RO1 HL 54727] FX The authors are grateful for the technical expertise provided by Susan Jalbert, and Drs. Donald Smith and Mohsen Meydani, and for the thoughtful manuscript review by Drs. Julian Marsh and Alice Dillard. This project was supported by T32 HL69772-01A1 (S. W., J.L.), RO1 HL 54727 and USDA agreement 588-1950-9-001. Any opinions, findings, conclusions or recommendations expressed in this publication are those of authors, and do not necessarily reflect the view of USDA. NR 56 TC 6 Z9 6 U1 1 U2 2 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD AUG PY 2010 VL 45 IS 8 BP 701 EP 711 DI 10.1007/s11745-010-3454-8 PG 11 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA 640DJ UT WOS:000281027800005 PM 20686867 ER PT J AU Burke, JM Orlik, S Miller, JE Terrill, TH Mosjidis, JA AF Burke, J. M. Orlik, S. Miller, J. E. Terrill, T. H. Mosjidis, J. A. TI Using copper oxide wire particles or sericea lespedeza to prevent peri-parturient gastrointestinal nematode infection in sheep and goats SO LIVESTOCK SCIENCE LA English DT Article DE Copper oxide; Gastrointestinal nematodes; Goat; Sericea lespedeza; Sheep ID HAEMONCHUS-CONTORTUS INFECTION; SOUTHEASTERN UNITED-STATES; COAST NATIVE SHEEP; FECAL EGG COUNTS; NATURAL DEWORMER; AFRICAN GOATS; RESISTANCE; LAMBS; HAY; SUSCEPTIBILITY AB Gastrointestinal nematodes (GIN) continue to plague the small ruminant industry, especially in parts of the world with warm, humid climates. Alternatives to chemicals are needed for GIN control because of anthelmintic resistance and a desire to reduce chemical residues in meat products. Three experiments using peri-parturient does or ewes addressed the objective: 1) in Arkansas, meat goats were untreated (n=20) or fed copper oxide wire particles (COWP: 2 g each) in pelleted sericea lespedeza (Lespedeza cuneata: n=22) before kidding while consuming sericea lespedeza hay, 2) in Arkansas, 42 Katahdin ewes were randomly assigned to remain untreated or were fed COWP (2 g each) before lambing within groups fed bermudagrass (Cynodon dactylon) or sericea lespedeza hay in a 2 x 2 factorial design, 3) in Louisiana, Gulf Coast Native ewes were randomly assigned to remain untreated or were fed COWP (4 g each) in a pelleted ration (n=10 each) after lambing began. Fecal egg counts (FEC) and blood packed cell volume (PCV) were determined weekly in all experiments, and coproculture to examine GIN species was conducted in the first two experiments. Haemonchus contort us is typically the predominant GIN in the southeastern U.S., even during cooler months. However. Trichostrongylus spp. was the predominant GIN in Arkansas during these experiments. In all of the experiments, feeding COWP led to a reduction in FEC, but no change in PCV. The sericea lespedeza hay fed to ewes in Experiment 2 was associated with a reduction in FEC compared with ewes fed bermudagrass hay. Kids and lambs from COWP-treated dams in two experiments were lighter than those from untreated dams. Sericea lespedeza aided in the control of GIN infection, and while feeding COWP to peri-parturient ewes and does offered some reduction in GIN infection, body weight of offspring at birth and 60 or 90 days of age may be reduced. Published by Elsevier B.V. C1 [Burke, J. M.] ARS, Dale Bumpers Small Farms Res Ctr, USDA, Booneville, AR 72927 USA. [Orlik, S.; Miller, J. E.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Terrill, T. H.] Ft Valley State Univ, Ft Valley, GA 31030 USA. [Mosjidis, J. A.] Auburn Univ, Auburn, AL 36849 USA. RP Burke, JM (reprint author), ARS, Dale Bumpers Small Farms Res Ctr, USDA, Booneville, AR 72927 USA. EM joan.burke@ars.usda.gov FU Southern Region SARE Program [LS08-204] FX This research was supported by the Southern Region SARE Program (Project No. LS08-204). The authors greatly appreciate the efforts of G. Robson, J. Cherry, and S. Moore for technical assistance. NR 36 TC 5 Z9 5 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 J9 LIVEST SCI JI Livest. Sci. PD AUG PY 2010 VL 132 IS 1-3 BP 13 EP 18 DI 10.1016/j.livsci.2010.04.015 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 638YT UT WOS:000280937300002 ER PT J AU Waldbieser, GC Bosworth, BG Quiniou, SMA AF Waldbieser, Geoffrey C. Bosworth, Brian G. Quiniou, Sylvie M. A. TI Production of Viable Homozygous, Doubled Haploid Channel Catfish (Ictalurus punctatus) SO MARINE BIOTECHNOLOGY LA English DT Article DE Channel; Catfish; Homozygous; Doubled haploid; Gynogenesis ID RAINBOW-TROUT; HEAT-SHOCK; CLONES; FISH; GYNOGENESIS; MANIPULATION; CLEAVAGE AB Production of doubled haploids via mitotic gynogenesis is a useful tool for the creation of completely inbred fish. In order to produce viable doubled haploid channel catfish, we utilized hydrostatic pressure or thermal treatments on eggs fertilized with sperm that had been exposed to ultraviolet light. At 1.5 h post-fertilization, the embryos were exposed to either 590 kg/cm(2) hydrostatic pressure for 3 min, 37A degrees C for 5 min, or 41A degrees C for 3 min. In the pressure-treated group, only 21 offspring hatched from five spawns with family sizes of one, two, two, four, and 12 offspring each. Eight embryos from the 37A degrees C treatment and 32 embryos from the 41A degrees C treatment survived to hatch. Genotype analysis using microsatellite loci demonstrated all 21 offspring resulting from pressure treatment were homozygous at the 64 loci tested, and none contained alleles unique to the donor male. Eleven of 32 offspring from the 41A degrees C treatment were homozygous at the 18 loci tested, while 21 offspring were heterozygous at six to 12 of these loci. Again, no offspring contained alleles unique to the donor male. However, all eight offspring from the 37A degrees C treatment were heterozygous at multiple loci, and one contained unambiguous paternal alleles. These experiments demonstrated our ability to produce viable homozygous, doubled haploid channel catfish. Doubled haploid catfish can be used to create completely inbred populations for genetic analyses, and homozygous genomic templates will be useful in gene identification and genome characterization. C1 [Waldbieser, Geoffrey C.; Bosworth, Brian G.; Quiniou, Sylvie M. A.] ARS, Catfish Genet Res Unit, USDA, Stoneville, MS 38776 USA. RP Waldbieser, GC (reprint author), ARS, Catfish Genet Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM Geoff.Waldbieser@ars.usda.gov NR 23 TC 14 Z9 14 U1 0 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-2228 J9 MAR BIOTECHNOL JI Mar. Biotechnol. PD AUG PY 2010 VL 12 IS 4 BP 380 EP 385 DI 10.1007/s10126-009-9221-2 PG 6 WC Biotechnology & Applied Microbiology; Marine & Freshwater Biology SC Biotechnology & Applied Microbiology; Marine & Freshwater Biology GA 630JX UT WOS:000280270300002 PM 19707826 ER PT J AU Ribic, CA Sheavly, SB Rugg, DJ Erdmann, ES AF Ribic, Christine A. Sheavly, Seba B. Rugg, David J. Erdmann, Eric S. TI Trends and drivers of marine debris on the Atlantic coast of the United States 1997-2007 SO MARINE POLLUTION BULLETIN LA English DT Article DE Marine debris; Beaches; Atlantic coast; United States; Trend; Monitoring ID TEMPORAL VARIATIONS; BEACH DEBRIS; NEW-JERSEY; LITTER; DEPOSITION; AUSTRALIA; ISLANDS; REGION AB For the first time, we documented regional differences in amounts and long-term trends of marine debris along the US Atlantic coast. The Southeast Atlantic had low land-based and general-source debris loads as well as no increases despite a 19% increase in coastal population. The Northeast (8% population increase) also had low land-based and general-source debris loads and no increases. The Mid-Atlantic (10% population increase) fared the worst, with heavy land-based and general-source debris loads that increased over time. Ocean-based debris did not change in the Northeast where the fishery is relatively stable; it declined over the Mid-Atlantic and Southeast and was correlated with declining regional fisheries. Drivers, including human population, land use status, fishing activity, and oceanic current systems, had complex relationships with debris loads at local and regional scales. Management challenges remain undeniably large but solid information from long-term programs is one key to addressing this pressing pollution issue. Published by Elsevier Ltd. C1 [Ribic, Christine A.] Univ Wisconsin, US Geol Survey, Wisconsin Cooperat Wildlife Res Unit, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Sheavly, Seba B.] Sheavly Consultants, Virginia Beach, VA 23452 USA. [Rugg, David J.] US Forest Serv, No Res Stn, Madison, WI 53726 USA. [Erdmann, Eric S.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Ribic, CA (reprint author), Univ Wisconsin, US Geol Survey, Wisconsin Cooperat Wildlife Res Unit, Dept Forest & Wildlife Ecol, 218 Russell Labs,1630 Linden Dr, Madison, WI 53706 USA. EM caribic@wisc.edu; seba@sheavlyconsultants.com; drugg@fs.fed.us; e1erdmann@gmail.com OI Rugg, David/0000-0003-2280-8302 FU US Department of Commerce National Oceanic and Atmospheric Administration Marine Entanglement Research Program; US Environmental Protection Agency (EPA) Office of Water through the Center for Marine Conservation; US EPA Office of Water through the Ocean Conservancy; US Geological Survey Cooperative Research Units; US Forest Service FX This paper would not have been possible without the many volunteers and organizations that collected the data; we sincerely thank all who participated in the surveys. In particular we thank Dr. Gayle Kraus (University of ME-Machias), Ted and Paula Merritt, Jen Kennedy (Blue Ocean Society for Marine Conservation), Joe Cravalho (MA Beach Buggy Association), Sharon Sneed (Pen Bay Stewards), Carl Rasmussen and Russell Moehlich (Friends of Cape Cod National Seashore), Mary Merges, Suzan Bellincampi and Sarah Trudel (The Trustees of Reservation), Scott Comings (The Nature Conservancy), Bob Glover and Jack Isaacs (Audubon Society), Barbara Boyd and students (Marine Academy of Science and Technology), Carol Elliot and Tom Sherman (Alliance for a Living Ocean), Earl and Faith Chamberlin (DE Mobile Surf Fishermen), Bill Lewis (Strathmere Fishing and Environmental Club), Fred Pulls and Geralyn Mirales (Chincoteague NWR), Dr. Gail Cannon and Zoe Meletis (Duke Marine Laboratory), Fred Hay and Susan Johnston (Sapelo Island NERR), Cathy Marsh and Ann Arnold (Volusia Flagler Sierra Club), Liz Melvin and Jim Kriewaldt (Keep Brevard Beautiful) and Lynn Emerson and Andrea Povinelli (The Nature Conservancy, Blowing Rocks Reserve) for their efforts in carrying out monthly data collections for over 10 years. The US Department of Commerce National Oceanic and Atmospheric Administration Marine Entanglement Research Program and the US Environmental Protection Agency (EPA) Office of Water funded the workshops through the Center for Marine Conservation (now the Ocean Conservancy) that led to the development of the National Marine Debris Monitoring Program. The US EPA Office of Water funded data collection through the Ocean Conservancy. The US Geological Survey Cooperative Research Units Program and the US Forest Service funded the analysis of the data used in this paper. Mention of trade names or commercial products does not constitute endorsement for use by the US Government. We thank the Department of Forest and Wildlife Ecology, University of Wisconsin, Madison, for assistance with publication expenses. NR 50 TC 35 Z9 37 U1 2 U2 27 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0025-326X EI 1879-3363 J9 MAR POLLUT BULL JI Mar. Pollut. Bull. PD AUG PY 2010 VL 60 IS 8 BP 1231 EP 1242 DI 10.1016/j.marpolbul.2010.03.021 PG 12 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 645PC UT WOS:000281474000023 PM 20378132 ER PT J AU Jago, R Drews, KL Mcmurray, RG Thompson, D Volpe, SL Moe, EL Jakicic, JM Pham, TH Bruecker, S Blackshear, TB Yin, ZN AF Jago, Russell Drews, Kimberly L. Mcmurray, Robert G. Thompson, Debbe Volpe, Stella L. Moe, Esther L. Jakicic, John M. Pham, Trang H. Bruecker, Steve Blackshear, Tara B. Yin, Zenong TI Fatness, Fitness, and Cardiometabolic Risk Factors among Sixth-Grade Youth SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article DE FIT; OBESITY; CHILDREN; ADOLESCENTS; CARDIOVASCULAR DISEASE ID CARDIOVASCULAR-DISEASE RISK; NUTRITION EXAMINATION SURVEY; SHUTTLE RUN TEST; PHYSICAL-ACTIVITY; CARDIORESPIRATORY FITNESS; METABOLIC SYNDROME; AEROBIC FITNESS; BODY-COMPOSITION; NATIONAL-HEALTH; BLOOD-PRESSURE AB JAGO, R., K. L. DREWS, R. G. MCMURRAY, D. THOMPSON, S. L. VOLPE, E. L. MOE, J. M. JAKICIC, T. H. PHAM, S. BRUECKER, T. B. BLACKSHEAR, and Z. YIN. Fatness, Fitness, and Cardiometabolic Risk Factors among Sixth-Grade Youth. Med. Sci. Sports Exerc., Vol. 42, No. 8, pp. 1502-1510, 2010. Purpose: Examine whether cardiometabolic risk factors are predicted by fitness or fatness among adolescents. Methods: Participants are 4955 (2614 female) sixth-grade students with complete data from 42 US middle schools. Fasting blood samples were analyzed for total cholesterol, HDL- and LDL-cholesterol, triglyceride, glucose, and insulin concentrations. Waist circumference and blood pressure were assessed. Body mass index (BMI) was categorized as normal weight, overweight, or obese as a measure of fatness. Fitness was assessed using the multistage shuttle test and was converted into gender-specific quintiles. Gender-specific regression models, adjusted for race, pubertal status, and household education, were run to identify whether BMI group predicted risk factors. Models were repeated with fitness group and both fitness and fatness groups as predictors. Results: Means for each risk factor (except HDL, which was the reverse) were significantly higher (P < 0.0001) with increased fatness and differed across all BMI groups (P < 0.001). Waist circumference, LDL-cholesterol, triglycerides, diastolic blood pressure, and insulin were inversely associated with fitness (P < 0.001). When both fatness and fitness were included in the model, BMI was associated (P < 0.001) with almost all cardiometabolic risk factors; fitness was only associated with waist circumference (both genders), LDL-cholesterol (males), and insulin (both genders). Other associations between fitness and cardiometabolic risk factors were attenuated after adjustment for BMI group. Conclusions: Both fatness and fitness are associated with cardiometabolic risk factors among sixth-grade youth, but stronger associations were observed for fatness. Although maintaining high levels of fitness and preventing obesity may positively affect cardiometabolic risk factors, greater benefit may be obtained from obesity prevention. C1 [Jago, Russell] Univ Bristol, Dept Exercise Nutr & Hlth Sci, Ctr Sport Exercise & Hlth, Bristol BS8 1TP, Avon, England. [Drews, Kimberly L.; Pham, Trang H.] George Washington Univ, Ctr Biostat, Washington, DC USA. [Mcmurray, Robert G.] Univ N Carolina, Dept Exercise & Sport Sci, Chapel Hill, NC USA. [Thompson, Debbe] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Volpe, Stella L.] Univ Penn, Sch Nursing, Div Biobehav & Hlth Sci, Philadelphia, PA 19104 USA. [Moe, Esther L.] Oregon Hlth & Sci Univ, Div Hlth Promot & Sports Med, Portland, OR 97201 USA. [Jakicic, John M.] Univ Pittsburgh, Dept Hlth & Phys Act, Pittsburgh, PA USA. [Bruecker, Steve] Univ Calif Irvine, Dept Hlth & Phys Act, Irvine, CA USA. [Blackshear, Tara B.] Cairo Amer Coll, Cairo, Egypt. [Yin, Zenong] Univ Texas San Antonio, Dept Hlth & Kinesiol, San Antonio, TX USA. RP Jago, R (reprint author), Univ Bristol, Dept Exercise Nutr & Hlth Sci, Ctr Sport Exercise & Hlth, Tyndall Ave, Bristol BS8 1TP, Avon, England. EM russ.jago@gmail.com OI Moe, Esther/0000-0002-9491-9741; Jago, Russell/0000-0002-3394-0176 FU National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health [U01-DK61230, U01-DK61249, U01-DK61231, U01-DK61223]; American Diabetes Association FX This work was completed with funding from National Institute of Diabetes and Digestive and Kidney Diseases/National Institutes of Health grant numbers U01-DK61230, U01-DK61249, U01-DK61231, and U01-DK61223 to the STOPP-T2D collaborative group, with additional support from the American Diabetes Association. NR 41 TC 23 Z9 23 U1 0 U2 7 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD AUG PY 2010 VL 42 IS 8 BP 1502 EP 1510 DI 10.1249/MSS.0b013e3181d322c4 PG 9 WC Sport Sciences SC Sport Sciences GA 626PH UT WOS:000279977500010 PM 20139783 ER PT J AU Britch, SC Linthicum, KJ Wynn, WW Walker, TW Farooq, M Smith, VL Robinson, CA Lothrop, BB Snelling, M Gutierrez, A Lothrop, HD AF Britch, Seth C. Linthicum, Kenneth J. Wynn, Wayne W. Walker, Todd W. Farooq, Muhammad Smith, Vincent L. Robinson, Cathy A. Lothrop, Branka B. Snelling, Melissa Gutierrez, Arturo Lothrop, Hugh D. TI Residual Mosquito Barrier Treatments on US Military Camouflage Netting in a Southern California Desert Environment SO MILITARY MEDICINE LA English DT Article; Proceedings Paper CT 75th Annual Meeting of the American-Mosquito-Control-Association CY APR 06, 2009 CL New Orleans, LA SP Amer Mosquito Control Assoc ID FIELD-EVALUATION; MALARIA VECTORS; INSECTICIDE; BIFENTHRIN; PERMETHRIN; PROTECTION; VEGETATION; AUSTRALIA; MALATHION; DIPTERA AB Treating perimeters of vegetation with residual insecticides for protection from mosquito vectors has potential for U.S. military force health protection. However, for current U.S. military operations in hot-arid environments with little or no vegetation, residual applications on portable artificial materials may be a viable alternative. We evaluated bifenthrin residual treatments of U.S. military camouflage netting under hot-arid field conditions in a desert area in southern California exposed to abundant wild Culex tarsalis mosquitoes. We assessed the ability of the treatment to reduce the numbers of mosquitoes penetrating perimeters of netting and reaching CO(2)-baited mosquito traps. Treated camouflage netting barriers reduced mosquitoes by >= 50% for 7-14 days and by 20-35% for 21-28 days compared to untreated barriers. Although reductions may be translated into reductions in risk of exposure to mosquito-borne diseases, we emphasize that barrier treatments should be a component in a suite of insect control measures to be effective. C1 [Britch, Seth C.; Linthicum, Kenneth J.; Wynn, Wayne W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Walker, Todd W.; Farooq, Muhammad; Smith, Vincent L.; Robinson, Cathy A.] USN, Air Stn, Navy Entomol Ctr Excellence, Jacksonville, FL 32212 USA. [Lothrop, Branka B.; Snelling, Melissa; Gutierrez, Arturo] Coachella Valley Mosquito & Vector Control Dist, Indio, CA 92201 USA. [Lothrop, Hugh D.] Univ Calif Davis, Sch Vet Med, Ctr Vectorborne Dis, Arbovirus Res Unit, Davis, CA 95616 USA. RP Britch, SC (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 44 TC 6 Z9 6 U1 1 U2 1 PU ASSOC MILITARY SURG US PI BETHESDA PA 9320 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0026-4075 J9 MIL MED JI Milit. Med. PD AUG PY 2010 VL 175 IS 8 BP 599 EP 606 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA 632VB UT WOS:000280456000012 PM 20731265 ER PT J AU Vijayalakshmi, K Fritz, AK Paulsen, GM Bai, GH Pandravada, S Gill, BS AF Vijayalakshmi, Kolluru Fritz, Allan K. Paulsen, Gary M. Bai, Guihua Pandravada, Satchidanand Gill, Bikram S. TI Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature SO MOLECULAR BREEDING LA English DT Article DE Post-anthesis heat stress; Senescence; Modeling; Molecular markers; Marker-assisted breeding; QTL mapping ID BICOLOR L. MOENCH; STAY GREEN TRAIT; GRAIN-SORGHUM; LEAF SENESCENCE; DROUGHT RESISTANCE; LOCI; TOLERANCE; RICE; ENVIRONMENTS; PROTEIN AB Senescence is a genetically programmed and environmentally influenced process resulting in the destruction of chlorophyll and remobilization of nutrients to younger or reproductive parts of plants. Delayed senescence, or stay-green, contributes to a long grain-filling period and stable yield under stress. To model senescence and identify quantitative trait loci (QTL) for the trait, a population of recombinant inbred lines (RIL) from a cross between winter wheat cultivars, 'Ventnor' and 'Karl 92' was evaluated for heat tolerance under optimum temperature of 20/15A degrees C (day/night) and continuous heat stress of 30/25A degrees C from 10 days after anthesis (DAA) until maturity. Ventnor is a heat-tolerant cultivar and Karl 92 is a relatively heat-susceptible cultivar. Green leaf area was measured and used to model percent greenness retained over the reproductive period. Chlorophyll content and chlorophyll fluorescence were recorded on flag leaves. Senescence was converted to a quantitative trait using the model. Based on the modeled parameters, the RILs were categorized into three groups. When senescence-related traits were evaluated, nine QTL for heat tolerance were found on chromosome 2A, two each on chromosomes 6A and 6B and one each on chromosome 3A, 3B, and 7A. Both parents contributed favorable alleles for most of the senescence-related traits. Microsatellite markers Xgwm356 and Xgwm5 prominently linked to the senescence-related traits may be useful in marker-assisted breeding. These and the linked AFLP (amplified fragment length polymorphism) markers XCGT.TGCG-349, XCGT.GTG-343, and XCGT.CTCG-406, if converted to STS (sequence tagged sites), can be used for further molecular dissection of the QTL for post-anthesis heat tolerance. C1 [Fritz, Allan K.; Bai, Guihua] Kansas State Univ, Dept Agron, USDA ARS, Manhattan, KS 66506 USA. [Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Pandravada, Satchidanand] Pioneer Overseas Corp, Gauribidanur Taluk, Karnataka, India. RP Fritz, AK (reprint author), Kansas State Univ, Dept Agron, USDA ARS, Manhattan, KS 66506 USA. EM akf@ksu.edu FU Kansas Wheat Commission FX We thank Kansas Wheat Commission for financial support of the project. We especially thank Dr. George Milliken, department of statistics at Kansas State University for his help in modeling the data. We would also like to thank Drs. Mitch Tuinstra and Paul St. Amand for extending their laboratory facilities. NR 47 TC 42 Z9 47 U1 2 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2010 VL 26 IS 2 SI SI BP 163 EP 175 DI 10.1007/s11032-009-9366-8 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630CF UT WOS:000280248400004 ER PT J AU Maccaferri, M Sanguineti, MC Mantovani, P Demontis, A Massi, A Ammar, K Kolmer, JA Czembor, JH Ezrati, S Tuberosa, R AF Maccaferri, Marco Sanguineti, Maria C. Mantovani, Paola Demontis, Andrea Massi, Andrea Ammar, Karim Kolmer, James A. Czembor, Jerzy H. Ezrati, Smadar Tuberosa, Roberto TI Association mapping of leaf rust response in durum wheat SO MOLECULAR BREEDING LA English DT Article DE Durum wheat; Puccinia triticina Eriks; Association mapping; Linkage disequilibrium; Quantitative trait locus; Molecular marker ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; WIDE LINKAGE DISEQUILIBRIUM; MULTILOCUS GENOTYPE DATA; BREAD WHEAT; POPULATION-STRUCTURE; PUCCINIA-TRITICINA; RESISTANCE GENE; PEDIGREE INFORMATION; DISEASE RESISTANCE AB Resistance to leaf rust (Puccinia triticina Eriks.) is a main objective for durum wheat (Triticum durum Desf.) breeding. Association mapping on germplasm collections is now being used as an additional approach for the discovery and validation of major genes/QTLs. In this study, a collection of 164 elite durum wheat accessions suitable for association mapping has been tested for leaf rust response at the seedling stage and under field conditions (adult plant stage). Seedling tests were carried out with 25 selected isolates from durum wheat, bread wheat and triticale, while field experiments were carried out in artificially inoculated plots in Italy and in Mexico. The collection has been profiled with 225 simple sequence repeat (SSR) loci of known map position and a PCR assay targeting Ppd-A1. Associations showing highly consistent experiment-wise significances across leaf rust isolates and field trials were mainly detected for the 7BL distal chromosome (chr.) region (harbouring Lr14 from cultivar Llareta INIA and QLr.ubo-7B.2 from cultivar Creso) and for two chr. regions located in chrs. 2A and 2B. Additionally, isolate-specific associations and/or associations with smaller effects in the field trials were identified in most of the chromosomes. The chr. 7BL distal region was investigated in detail through haplotyping with 15 SSR markers, revealing that the Creso and Llareta INIA alleles are identical by descent at 6 adjacent SSR loci in the most distal 7BL region spanning 8 cM. Association mapping allowed us to further refine the map location of the Lr14/QLr.ubo-7B.2 resistance gene to the most distal region of the linkage group, tagged by Xcfa2257.2, Xgwm344.2 and Xwmc10. The resistant haplotype is present in a number of accessions (ca. 15% of the accessions included in the collection) from the Italian, CIMMYT and ICARDA breeding programmes. Therefore, this chr. 7BL region can be considered as the most important source of resistance to leaf rust currently exploited by durum breeders in the Mediterranean areas. Furthermore, the field trials at the adult plant stage allowed us to identify marker associations (e.g. chrs. 2BL and 3BS, proximal regions; chr. 7BS, distal region) which suggest the presence of minor QTLs for slow-rusting resistance. C1 [Maccaferri, Marco; Sanguineti, Maria C.; Mantovani, Paola; Tuberosa, Roberto] Univ Bologna, Dept Agroenvironm Sci & Technol, I-40127 Bologna, Italy. [Demontis, Andrea; Massi, Andrea] Soc Produttori Sementi Bologna, I-40050 Argelato, Italy. [Ammar, Karim] CIMMYT, Texcoco 56134, Mexico. [Kolmer, James A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Czembor, Jerzy H.] IHAR, Dept Genet & Plant Breeding, PL-05870 Blonie, Poland. [Ezrati, Smadar] Tel Aviv Univ, Dept Plant Sci, IL-69978 Tel Aviv, Israel. RP Sanguineti, MC (reprint author), Univ Bologna, Dept Agroenvironm Sci & Technol, I-40127 Bologna, Italy. EM maria.sanguineti@unibo.it RI Czembor, Jerzy/I-8188-2015; Tuberosa, Roberto/A-2781-2016 OI Tuberosa, Roberto/0000-0001-9143-9569 FU European Union; Emilia-Romagna Region FX Research carried out with the financial contribution of the European Union (BIOEXPLOIT project) and of the Emilia-Romagna Region (CEREALAB-SITEIA project). The contribution of the EU-funded IDuWUE project and the IDuWUE Research Consortium in providing part of the tested materials and microsatellite markers is gratefully acknowledged. The technical assistance of Sandra Stefanelli is also gratefully acknowledged. NR 93 TC 41 Z9 43 U1 3 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2010 VL 26 IS 2 SI SI BP 189 EP 228 DI 10.1007/s11032-009-9353-0 PG 40 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630CF UT WOS:000280248400006 ER PT J AU Roy, JK Smith, KP Muehlbauer, GJ Chao, SM Close, TJ Steffenson, BJ AF Roy, Joy K. Smith, Kevin P. Muehlbauer, Gary J. Chao, Shiaoman Close, Timothy J. Steffenson, Brian J. TI Association mapping of spot blotch resistance in wild barley SO MOLECULAR BREEDING LA English DT Article DE Hordeum vulgare subsp spontaneum; Quantitative trait loci (QTL); Population structure; Linkage disequilibrium mapping; SNP; DArT (R) ID MULTIPLE DISEASE RESISTANCE; MULTILOCUS GENOTYPE DATA; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; COCHLIOBOLUS-SATIVUS; POPULATION-STRUCTURE; FLOWERING TIME; ARABIDOPSIS-THALIANA; HAPLOTYPE DIVERSITY; NORTH-DAKOTA AB Spot blotch, caused by Cochliobolus sativus, is an important foliar disease of barley. The disease has been controlled for over 40 years through the deployment of cultivars with durable resistance derived from the line NDB112. Pathotypes of C. sativus with virulence for the NDB112 resistance have been detected in Canada; thus, many commercial cultivars are vulnerable to spot blotch epidemics. To increase the diversity of spot blotch resistance in cultivated barley, we evaluated 318 diverse wild barley accessions comprising the Wild Barley Diversity Collection (WBDC) for reaction to C. sativus at the seedling stage and utilized an association mapping (AM) approach to identify and map resistance loci. A high frequency of resistance was found in the WBDC as 95% (302/318) of the accessions exhibited low infection responses. The WBDC was genotyped with 558 Diversity Array Technology (DArT(A (R))) and 2,878 single nucleotide polymorphism (SNP) markers and subjected to structure analysis before running the AM procedure. Thirteen QTL for spot blotch resistance were identified with DArT and SNP markers. These QTL were found on chromosomes 1H, 2H, 3H, 5H, and 7H and explained from 2.3 to 3.9% of the phenotypic variance. Nearly half of the identified QTL mapped to chromosome bins where spot blotch resistance loci were previously reported, offering some validation for the AM approach. The other QTL mapped to unique genomic regions and may represent new spot blotch resistance loci. This study demonstrates that AM is an effective technique for identifying and mapping QTL for disease resistance in a wild crop progenitor. C1 [Roy, Joy K.; Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Smith, Kevin P.; Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Close, Timothy J.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. RP Steffenson, BJ (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM bsteffen@umn.edu OI Steffenson, Brian/0000-0001-7961-5363 NR 54 TC 65 Z9 70 U1 1 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2010 VL 26 IS 2 SI SI BP 243 EP 256 DI 10.1007/s11032-010-9402-8 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630CF UT WOS:000280248400008 ER PT J AU Prasanna, BM Pixley, K Warburton, ML Xie, CX AF Prasanna, B. M. Pixley, Kevin Warburton, Marilyn L. Xie, Chuan-Xiao TI Molecular marker-assisted breeding options for maize improvement in Asia SO MOLECULAR BREEDING LA English DT Article DE Maize; Breeding; Markers; Genetic diversity; QTL; Stress resistance; Quality ID QUANTITATIVE TRAIT LOCI; ESSENTIALLY DERIVED VARIETIES; QUALITY PROTEIN MAIZE; QTLS CONFERRING RESISTANCE; SIMPLE SEQUENCE REPEAT; INBRED LINES; GENETIC DIVERSITY; CHINESE MAIZE; DOWNY MILDEW; LINKAGE DISEQUILIBRIUM AB Maize is one of the most important food and feed crops in Asia, and is a source of income for several million farmers. Despite impressive progress made in the last few decades through conventional breeding in the "Asia-7" (China, India, Indonesia, Nepal, Philippines, Thailand, and Vietnam), average maize yields remain low and the demand is expected to increasingly exceed the production in the coming years. Molecular marker-assisted breeding is accelerating yield gains in USA and elsewhere, and offers tremendous potential for enhancing the productivity and value of Asian maize germplasm. We discuss the importance of such efforts in meeting the growing demand for maize in Asia, and provide examples of the recent use of molecular markers with respect to (i) DNA fingerprinting and genetic diversity analysis of maize germplasm (inbreds and landraces/OPVs), (ii) QTL analysis of important biotic and abiotic stresses, and (iii) marker-assisted selection (MAS) for maize improvement. We also highlight the constraints faced by research institutions wishing to adopt the available and emerging molecular technologies, and conclude that innovative models for resource-pooling and intellectual-property-respecting partnerships will be required for enhancing the level and scope of molecular marker-assisted breeding for maize improvement in Asia. Scientists must ensure that the tools of molecular marker-assisted breeding are focused on developing commercially viable cultivars, improved to ameliorate the most important constraints to maize production in Asia. C1 [Prasanna, B. M.] Indian Agr Res Inst, Div Genet, New Delhi 110012, India. [Pixley, Kevin] Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco, Mexico. [Pixley, Kevin] Univ Wisconsin, Madison, WI USA. [Warburton, Marilyn L.] USDA ARS, CHPRRU, Mississippi State, MS 39762 USA. [Xie, Chuan-Xiao] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China. RP Prasanna, BM (reprint author), Indian Agr Res Inst, Div Genet, New Delhi 110012, India. EM bmprasanna@gmail.com NR 130 TC 31 Z9 34 U1 2 U2 31 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2010 VL 26 IS 2 SI SI BP 339 EP 356 DI 10.1007/s11032-009-9387-3 PG 18 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630CF UT WOS:000280248400014 ER PT J AU Nagy, ED Chu, Y Guo, YF Khanal, S Tang, SX Li, Y Dong, WBB Timper, P Taylor, C Ozias-Akins, P Holbrook, CC Beilinson, V Nielsen, NC Stalker, HT Knapp, SJ AF Nagy, Ervin D. Chu, Ye Guo, Yufang Khanal, Sameer Tang, Shunxue Li, Yan Dong, Weibo B. Timper, Patricia Taylor, Christopher Ozias-Akins, Peggy Holbrook, C. Corley Beilinson, Vadim Nielsen, Niels C. Stalker, H. Thomas Knapp, Steven J. TI Recombination is suppressed in an alien introgression in peanut harboring Rma, a dominant root-knot nematode resistance gene SO MOLECULAR BREEDING LA English DT Article DE Arachis; Meloidogyne; Marker-assisted selection; Fabaceae; Nucleotide binding site leucine-rich repeat; Receptor-like kinase ID ARACHIS-HYPOGAEA L.; PLANT-DISEASE RESISTANCE; ORYZAE PV. ORYZAE; CULTIVATED PEANUT; MELOIDOGYNE-ARENARIA; BOTANICAL VARIETIES; GERMPLASM LINES; NUCLEIC-ACID; WILD ARACHIS; LINKAGE MAP AB Rma, a dominant root-knot nematode resistance gene introduced into tetraploid peanut (Arachis hypogaea) from a synthetic allotetraploid donor (TxAG-6), has been widely deployed in modern cultivars. The genomic location and borders of the alien chromosome segment introgressed from TxAG-6 into NemaTAM (a BC(7)-derived introgression line) and other modern cultivars carrying Rma have not been genetically mapped, and resistance gene candidates (RGCs) have not been identified for Rma. Our study focused on densely populating the alien introgression with codominant DNA markers, identifying and mapping the borders of the alien introgression carried by NemaTAM, and identifying RGCs for Rma. Altogether, 2,847 simple sequence repeat (SSR) and 380 single strand conformational polymorphism (SSCP) markers were screened for linkage to Rma-247 of the SSCP markers targeted 202 nucleotide binding site (NBS) leucine-rich repeat (LRR) and other resistance (R) gene homologs (75 were identified by mining a peanut EST database). SSR, NBS-LRR, and Ser/Thr receptor-like protein loci within the alien introgression co-segregated with Rma in an F(4) population (Gregory x Tifguard) and were tightly linked and spanned 3.4 cM in an F(5) population (NemaTAM x GP-NC-WS-14). By comparative mapping in the A-genome progenitor of peanut (A. duranensis), Rma was discovered to have been introduced on an interstitial alien chromosome segment spanning one-third to one-half of chromosome 9A. Numerous codominant DNA markers were identified for finer mapping of Rma, shortening the alien introgression harboring Rma by marker-assisted selection, and introducing novel root-knot nematode R-genes into peanut by targeting syntenic segments on chromosomes 9A and 9B in wild diploid donors. C1 [Nagy, Ervin D.; Guo, Yufang; Khanal, Sameer; Tang, Shunxue; Li, Yan; Taylor, Christopher; Knapp, Steven J.] Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. [Chu, Ye; Ozias-Akins, Peggy] Univ Georgia, Dept Hort, Tifton, GA 31793 USA. [Dong, Weibo B.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Timper, Patricia; Holbrook, C. Corley] ARS, USDA, Tifton, GA 31793 USA. [Beilinson, Vadim; Nielsen, Niels C.; Stalker, H. Thomas] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Knapp, SJ (reprint author), Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. EM sjknapp@uga.edu RI Guo, Yufang/G-9363-2011 FU United States Department of Agriculture Plant Genome Program [2006-35604-17242]; National Peanut Board; Georgia Peanut Commodity Commission; Georgia Seed Development Commission; Peanut Foundation FX This research was supported by grants to S.J.K. from the United States Department of Agriculture Plant Genome Program (No. 2006-35604-17242) and S.J.K., P.O.A., H. T. S., C. C. H., and N.N. from the National Peanut Board, the Georgia Peanut Commodity Commission, the Georgia Seed Development Commission, and the Peanut Foundation. NR 71 TC 38 Z9 49 U1 1 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2010 VL 26 IS 2 SI SI BP 357 EP 370 DI 10.1007/s11032-010-9430-4 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630CF UT WOS:000280248400015 ER PT J AU Thurber, CS Reagon, M Gross, BL Olsen, KM Jia, YL Caicedo, AL AF Thurber, Carrie S. Reagon, Michael Gross, Briana L. Olsen, Kenneth M. Jia, Yulin Caicedo, Ana L. TI Molecular evolution of shattering loci in US weedy rice SO MOLECULAR ECOLOGY LA English DT Article DE abscission; candidate gene; Oryza sativa; red rice; seed dispersal ID QUANTITATIVE TRAIT LOCI; DOMESTICATION-RELATED TRAITS; ORYZA-SATIVA L.; RED RICE; SEED DORMANCY; LINKAGE DISEQUILIBRIUM; HYBRID POPULATIONS; STATISTICAL-METHOD; DNA POLYMORPHISM; IDENTIFICATION AB Cultivated rice fields worldwide are plagued with weedy rice, a conspecific weed of cultivated rice (Oryza sativa L.). The persistence of weedy rice has been attributed, in part, to its ability to shatter (disperse) seed prior to crop harvesting. In the United States, separately evolved weedy rice groups have been shown to share genomic identity with exotic domesticated cultivars. Here, we investigate the shattering phenotype in a collection of U.S. weedy rice accessions, as well as wild and cultivated relatives. We find that all U.S. weedy rice groups shatter seeds easily, despite multiple origins, and in contrast to a decrease in shattering ability seen in cultivated groups. We assessed allelic identity and diversity at the major shattering locus, sh4, in weedy rice; we find that all cultivated and weedy rice, regardless of population, share similar haplotypes at sh4, and all contain a single derived mutation associated with decreased seed shattering. Our data constitute the strongest evidence to date of an evolution of weeds from domesticated backgrounds. The combination of a shared cultivar sh4 allele and a highly shattering phenotype, suggests that U.S. weedy rice have re-acquired the shattering trait after divergence from their progenitors through alternative genetic mechanisms. C1 [Thurber, Carrie S.; Reagon, Michael; Caicedo, Ana L.] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. [Gross, Briana L.; Olsen, Kenneth M.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Jia, Yulin] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Caicedo, AL (reprint author), Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. EM caicedo@bio.umass.edu FU U.S. National Science Foundation [DBI-0638820] FX We are grateful to D. Gealy for providing weedy rice accessions and S.R. McCouch for providing several accessions used in this study. Members of the Caicedo lab provided comments that much improved that manuscript. This project was funded by a grant from the U.S. National Science Foundation Plant Genome Research Programme (DBI-0638820) to A.L.C., K.M.O. and Y.J. NR 55 TC 43 Z9 48 U1 2 U2 28 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2010 VL 19 IS 16 BP 3271 EP 3284 DI 10.1111/j.1365-294X.2010.04708.x PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 635OL UT WOS:000280664900004 PM 20584132 ER PT J AU da Silva, AG Eberhard, JR Wright, TF Avery, ML Russello, MA AF da Silva, Anders Goncalves Eberhard, Jessica R. Wright, Timothy F. Avery, Michael L. Russello, Michael A. TI Genetic evidence for high propagule pressure and long-distance dispersal in monk parakeet (Myiopsitta monachus) invasive populations SO MOLECULAR ECOLOGY LA English DT Article DE dispersal; invasion biology; monk parakeet; Myiopsitta monachus; parrots; population genetics; propagule pressure ID EXTRA-PAIR PATERNITY; NEW-ZEALAND; ESTABLISHMENT SUCCESS; REPRODUCTIVE SUCCESS; NATURAL-POPULATIONS; MICROSATELLITE LOCI; BIOLOGICAL INVASION; SPECIES INVASIONS; COMPUTER-PROGRAM; MATING SYSTEM AB The monk parakeet (Myiopsitta monachus) is a successful invasive species that does not exhibit life history traits typically associated with colonizing species (e.g., high reproductive rate or long-distance dispersal capacity). To investigate this apparent paradox, we examined individual and population genetic patterns of microsatellite loci at one native and two invasive sites. More specifically, we aimed at evaluating the role of propagule pressure, sexual monogamy and long-distance dispersal in monk parakeet invasion success. Our results indicate little loss of genetic variation at invasive sites relative to the native site. We also found strong evidence for sexual monogamy from patterns of relatedness within sites, and no definite cases of extra-pair paternity in either the native site sample or the examined invasive site. Taken together, these patterns directly and indirectly suggest that high propagule pressure has contributed to monk parakeet invasion success. In addition, we found evidence for frequent long-distance dispersal at an invasive site (similar to 100 km) that sharply contrasted with previous estimates of smaller dispersal distance made in the native range (similar to 2 km), suggesting long-range dispersal also contributes to the species' spread within the United States. Overall, these results add to a growing body of literature pointing to the important role of propagule pressure in determining, and thus predicting, invasion success, especially for species whose life history traits are not typically associated with invasiveness. C1 [da Silva, Anders Goncalves; Russello, Michael A.] Univ British Columbia Okanagan, Dept Biol, Kelowna, BC, Canada. [Eberhard, Jessica R.] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Eberhard, Jessica R.] Louisiana State Univ, Museum Nat Sci, Baton Rouge, LA 70803 USA. [Wright, Timothy F.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Avery, Michael L.] USDA, APHIS, WS Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL USA. RP da Silva, AG (reprint author), Univ British Columbia Okanagan, Dept Biol, 3333 Univ Way, Kelowna, BC, Canada. EM anders.goncalvesdasilva@csiro.au RI Goncalves da Silva, Anders/A-5172-2010; OI Goncalves da Silva, Anders/0000-0002-2257-8781 FU American Philosophical Society; Canadian Foundation for Innovation; National Institutes of Health [S06 GM008136]; United States Department of Agriculture FX We thank the Ortiz-Basualdo family and personnel at Estancia Santa Ana de Carpinchori and Estancia Palmira for their hospitality and assistance with fieldwork in Argentina. George Amato provided assistance during the early stages of this work. We would also like to acknowledge the contributions of J. Wright, D. Hoffmeier and J. Lindsay with Florida Power and Light Company, who provided monk parakeet samples and nest locations from south Florida; and M. Chandler, who provided monk parakeet samples and nest locations from Connecticut. We also thank Philippe Henry, Dany Garant and four anonymous reviewers, who provided constructive suggestions that improved the manuscript. Funding was provided by the American Philosophical Society (MAR), Canadian Foundation for Innovation (MAR), the National Institutes of Health grant number S06 GM008136 (TFW) and the United States Department of Agriculture (MLA). NR 80 TC 21 Z9 23 U1 4 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2010 VL 19 IS 16 BP 3336 EP 3350 DI 10.1111/j.1365-294X.2010.04749.x PG 15 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 635OL UT WOS:000280664900008 PM 20666997 ER PT J AU Gross, BL Reagon, M Hsu, SC Caicedo, AL Jia, YL Olsen, KM AF Gross, Briana L. Reagon, Michael Hsu, Shih-Chung Caicedo, Ana L. Jia, Yulin Olsen, Kenneth M. TI Seeing red: the origin of grain pigmentation in US weedy rice SO MOLECULAR ECOLOGY LA English DT Article DE crop-weed evolution; de-domestication; grain colour; Oryza sativa; proanthocyanidin; red rice ID ALS-INHIBITING HERBICIDES; ORYZA-SATIVA; SEED DORMANCY; CULTIVATED RICE; GENE FLOW; MAXIMUM-LIKELIHOOD; DNA POLYMORPHISM; RESISTANCE; POPULATIONS; DIVERSITY AB Weedy forms of crop species infest agricultural fields worldwide and are a leading cause of crop losses, yet little is known about how these weeds evolve. Red rice (Oryza sativa), a major weed of cultivated rice fields in the US, is recognized by the dark-pigmented grain that gives it its common name. Studies using neutral molecular markers have indicated a close relationship between US red rice and domesticated rice, suggesting that the weed may have originated through reversion of domesticated rice to a feral form. We have tested this reversion hypothesis by examining molecular variation at Rc, the regulatory gene responsible for grain pigmentation differences between domesticated and wild rice. Loss-of-function mutations at Rc account for the absence of proanthocyanidin pigments in cultivated rice grains, and the major rc domestication allele has been shown to be capable of spontaneous reversion to a functional form through additional mutations at the Rc locus. Using a diverse sample of 156 weedy, domesticated and wild Oryzas, we analysed DNA sequence variation at Rc and its surrounding 4 Mb genomic region. We find that reversion of domestication alleles does not account for the pigmented grains of weed accessions; moreover, we find that haplotypes characterizing the weed are either absent or very rare in cultivated rice. Sequences from genomic regions flanking Rc are consistent with a genomic footprint of the rc selective sweep in cultivated rice, and they are compatible with a close relationship of red rice to Asian Oryzas that have never been cultivated in the US. C1 [Gross, Briana L.; Hsu, Shih-Chung; Olsen, Kenneth M.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Reagon, Michael; Caicedo, Ana L.] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. [Jia, Yulin] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR USA. RP Olsen, KM (reprint author), Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA. EM kolsen@wustl.edu FU NIGMS NIH HHS [F32 GM082165-03] NR 50 TC 30 Z9 32 U1 2 U2 21 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2010 VL 19 IS 16 BP 3380 EP 3393 DI 10.1111/j.1365-294X.2010.04707.x PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 635OL UT WOS:000280664900011 PM 20584133 ER PT J AU Ibrahim, AS Gebremariam, T Lin, L Luo, GPS Husseiny, MI Skory, CD Fu, Y French, SW Edwards, JE Spellberg, B AF Ibrahim, Ashraf S. Gebremariam, Teclegiorgis Lin, Lin Luo, Guanpingsheng Husseiny, Mohamed I. Skory, Christopher D. Fu, Yue French, Samuel W. Edwards, John E., Jr. Spellberg, Brad TI The high affinity iron permease is a key virulence factor required for Rhizopus oryzae pathogenesis SO MOLECULAR MICROBIOLOGY LA English DT Article ID CELL TRANSPLANT RECIPIENTS; CANDIDA-ALBICANS STRAINS; SELECTABLE MARKER URA3; ASPERGILLUS-FUMIGATUS; SACCHAROMYCES-CEREVISIAE; IMPROVES SURVIVAL; CRYPTOCOCCUS-NEOFORMANS; STAPHYLOCOCCUS-AUREUS; HEME OXYGENASE; GENE ENCODES AB Rhizopus oryzae is the most common cause of mucormycosis, an angioinvasive fungal infection that causes more then 50% mortality rate despite first-line therapy. Clinical and animal model data clearly demonstrate that the presence of elevated available serum iron predisposes the host to mucormycosis. The high affinity iron permease gene (FTR1) is required for R. oryzae iron transport in iron-depleted environments. Here we demonstrate that FTR1 is required for full virulence of R. oryzae in mice. We show that FTR1 is expressed during infection in diabetic ketoacidosis (DKA) mice. In addition, we disrupted FTR1 by double cross-over homologous recombination, but multinucleated R. oryzae could not be forced to segregate to a homokaryotic null allele. Nevertheless, a reduction of the relative copy number of FTR1 and inhibition of FTR1 expression by RNAi compromised the ability of R. oryzae to acquire iron in vitro and reduced its virulence in DKA mice. Importantly, passive immunization with anti-Ftr1p immune sera protected DKA mice from infection with R. oryzae. Thus, FTR1 is a virulence factor for R. oryzae, and anti-Ftr1p passive immunotherapy deserves further evaluation as a strategy to improve outcomes of deadly mucormycosis. C1 [Ibrahim, Ashraf S.; Gebremariam, Teclegiorgis; Lin, Lin; Luo, Guanpingsheng; Husseiny, Mohamed I.; Fu, Yue; French, Samuel W.; Edwards, John E., Jr.] Univ Calif Los Angeles, Med Ctr, Los Angeles Biomed Res Inst Harbor, Div Infect Dis, Torrance, CA 90509 USA. [French, Samuel W.] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol, Los Angeles, CA 90095 USA. [Skory, Christopher D.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Spellberg, Brad] Harbor UCLA Med Ctr, Div Gen Internal Med, Torrance, CA 90509 USA. RP Ibrahim, AS (reprint author), Univ Calif Los Angeles, Med Ctr, Los Angeles Biomed Res Inst Harbor, Div Infect Dis, Torrance, CA 90509 USA. EM ibrahim@labiomed.org RI a, a/M-9467-2013 FU Public Health Service [R01 AI063503, R21 AI082414] FX The authors gratefully acknowledge the helpful discussions with Mingfu Liu and the technical assistance of May Abdallah and Mary Collins. Research described in this manuscript was conducted in part at the research facilities of the Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center. This work was supported by Public Health Service grant R01 AI063503 and R21 AI082414 to A.S.I. The authors have no conflict of interest to declare. NR 68 TC 30 Z9 32 U1 1 U2 5 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0950-382X J9 MOL MICROBIOL JI Mol. Microbiol. PD AUG PY 2010 VL 77 IS 3 BP 587 EP 604 DI 10.1111/j.1365-2958.2010.07234.x PG 18 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 628NZ UT WOS:000280128500007 PM 20545847 ER PT J AU Wiemann, P Brown, DW Kleigrewe, K Bok, JW Keller, NP Humpf, HU Tudzynski, B AF Wiemann, Philipp Brown, Daren W. Kleigrewe, Karin Bok, Jin Woo Keller, Nancy P. Humpf, Hans-Ulrich Tudzynski, Bettina TI FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulence SO MOLECULAR MICROBIOLOGY LA English DT Article ID GIBBERELLA-FUJIKUROI; ASPERGILLUS-NIDULANS; GENE-CLUSTER; SACCHAROMYCES-CEREVISIAE; SEXUAL DEVELOPMENT; PENICILLIN BIOSYNTHESIS; ACREMONIUM-CHRYSOGENUM; SCLEROTIAL PRODUCTION; NITROGEN REGULATION; FILAMENTOUS FUNGI AB P>Besides industrially produced gibberellins (GAs), Fusarium fujikuroi is able to produce additional secondary metabolites such as the pigments bikaverin and neurosporaxanthin and the mycotoxins fumonisins and fusarin C. The global regulation of these biosynthetic pathways is only poorly understood. Recently, the velvet complex containing VeA and several other regulatory proteins was shown to be involved in global regulation of secondary metabolism and differentiation in Aspergillus nidulans. Here, we report on the characterization of two components of the F. fujikuroi velvet-like complex, FfVel1 and FfLae1. The gene encoding this first reported LaeA orthologue outside the class of Eurotiomycetidae is upregulated in Delta Ffvel1 microarray-studies and FfLae1 interacts with FfVel1 in the nucleus. Deletion of Ffvel1 and Fflae1 revealed for the first time that velvet can simultaneously act as positive (GAs, fumonisins and fusarin C) and negative (bikaverin) regulator of secondary metabolism, and that both components affect conidiation and virulence of F. fujikuroi. Furthermore, the velvet-like protein FfVel2 revealed similar functions regarding conidiation, secondary metabolism and virulence as FfVel1. Cross-genus complementation studies of velvet complex component mutants between Fusarium, Aspergillus and Penicillium support an ancient origin for this complex, which has undergone a divergence in specific functions mediating development and secondary metabolism. C1 [Wiemann, Philipp; Tudzynski, Bettina] Univ Munster, Inst Bot, D-48149 Munster, Germany. [Wiemann, Philipp; Kleigrewe, Karin; Humpf, Hans-Ulrich] Univ Munster, Inst Lebensmittelchem, D-48149 Munster, Germany. [Brown, Daren W.] USDA ARS, Peoria, IL 61604 USA. [Bok, Jin Woo; Keller, Nancy P.] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA. [Keller, Nancy P.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Tudzynski, B (reprint author), Univ Munster, Inst Bot, Schlossgarten 3, D-48149 Munster, Germany. EM tudzynsb@uni-muenster.de RI Bok, Jin Woo/F-7140-2010; OI Wiemann, Philipp/0000-0002-7983-2218 FU Deutsche Forschungsgemeinschaft (DFG) [Tu1245/7]; NSF [MCB-0236393]; NIH [1 R01 Al065728-01] FX This research was supported by the Deutsche Forschungsgemeinschaft (DFG) (Tu1245/7) and work in Wisconsin was funded by NSF MCB-0236393 and NIH 1 R01 Al065728-01 to NPK. PW was holder of a research fellowship of the Graduiertenkolleg 1409 funded by the DFG. We thank Birgit Hoff and Ulrich Kuck (Bochum University) for cross-species complementation of P. chrysogenum mutants with the Fflae1 and Ffvel1 genes. We thank Birgitt Oeser for her help with FiRe analysis, Mark Busman for analysis of fumonisins, Thomas Briel, Janina Post, Stefanie Traeger and Helena Klamm for their help with mutant screens, BiFC vector construction and fusarin C analysis as well as Sabine Huber for excellent technical support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 85 TC 125 Z9 137 U1 4 U2 33 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0950-382X J9 MOL MICROBIOL JI Mol. Microbiol. PD AUG PY 2010 VL 77 IS 4 BP 972 EP 994 DI 10.1111/j.1365-2958.2010.07263.x PG 23 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 635QY UT WOS:000280672400013 PM 20572938 ER PT J AU Kumari, M Khazai, NB Ziegler, TR Nanes, MS Abrams, SA Tangpricha, V AF Kumari, Meena Khazai, Natasha B. Ziegler, Thomas R. Nanes, Mark S. Abrams, Steven A. Tangpricha, Vin TI Vitamin D-mediated calcium absorption in patients with clinically stable Crohn's disease: A pilot study SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Calcium; Crohn's disease; Malabsorption; TNF-alpha; Vitamin D ID INFLAMMATORY-BOWEL-DISEASE; NECROSIS-FACTOR-ALPHA; BONE-MINERAL DENSITY; RHEUMATOID-ARTHRITIS; INTESTINAL BIOPSIES; D DEFICIENCY; INFLIXIMAB; WOMEN; INTERLEUKIN-1-BETA; HOMEOSTASIS AB Vitamin D is the critical hormone for intestinal absorption of calcium. Optimal calcium absorption is important for proper mineralization of bone in the prevention of osteoporosis and osteoporotic fractures, among other important functions. Diseases associated with gut inflammation, such as Crohn's disease (CD), may impair calcium absorption. This pilot study evaluated vitamin D-dependent calcium absorption in subjects with CD. Male subjects with CD (n = 4) and healthy age-matched controls (n = 5) were studied. All subjects had fractional calcium absorption (FCA; by the dual calcium isotope method), serum 25-hydroxyvitamin D, serum calcium and 24h urinary calcium excretion measurements at baseline. The FCA in response to vitamin D therapy was re-assessed following administration of oral calcitriol 0.25 mcg twice daily for 1 wk, followed by oral calcitriol 0.50 mcg twice daily for 1 wk. Serum calcium and 24h urinary calcium determinations were re-assessed after each increasing dose of calcitriol as safety measures. There was no significant difference in calcium FCA at baseline or after increasing doses of calcitriol between the CD and controls. FCA in the control and CD group was approximately 35% at baseline, which increased to 60% after calcitriol therapy. No subject developed hypercalcemia or hypercalciuria. Our results suggest that CD patients have a normal response to vitamin D in enhancing the efficacy of calcium absorption. This suggests that stable CD patients can follow calcium and vitamin D guidelines of non-CD adults. Other factors independent of vitamin D status may impair intestinal calcium absorption in CD, including the degree and location of inflammation, presence of surgical resection and/or use of glucocorticoids. C1 [Kumari, Meena; Khazai, Natasha B.; Ziegler, Thomas R.; Nanes, Mark S.; Tangpricha, Vin] Emory Univ, Sch Med, Div Endocrinol Metab & Lipids, Dept Med, Atlanta, GA 30322 USA. [Ziegler, Thomas R.; Tangpricha, Vin] Emory Univ, Nutr & Hlth Sci Program, Grad Div Biol & Biomed Sci, Atlanta, GA 30322 USA. [Ziegler, Thomas R.; Tangpricha, Vin] Emory Univ, Sch Med, Ctr Clin & Mol Nutr, Dept Med, Atlanta, GA 30322 USA. [Nanes, Mark S.; Tangpricha, Vin] Atlanta VA Med Ctr, Decatur, GA USA. [Abrams, Steven A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Tangpricha, V (reprint author), Emory Univ, Sch Med, Div Endocrinol Metab & Lipids, Dept Med, 101 Woodruff Circle NE,WMRB 1301, Atlanta, GA 30322 USA. EM vin.tangpricha@emory.edu RI Tangpricha, Vin/A-8645-2009; OI Abrams, Steven/0000-0003-4972-9233; Tangpricha, Vin/0000-0002-4021-5853 FU Emory Center for Clinical and Molecular Nutrition, Emory University Research Committee, NIH [K23 AR054334]; Atlanta Research and Education Foundation; NIH [T32 DK007298, K24 RR023356] FX This study was supported by grants from the Emory Center for Clinical and Molecular Nutrition, Emory University Research Committee, NIH grant K23 AR054334 (VT) and Atlanta Research and Education Foundation (VT); NIH grant T32 DK007298 (M.K.) and NIH grant K24 RR023356 (T.R.Z.). NR 38 TC 9 Z9 9 U1 0 U2 4 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1613-4125 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD AUG PY 2010 VL 54 IS 8 BP 1085 EP 1091 DI 10.1002/mnfr.200900351 PG 7 WC Food Science & Technology SC Food Science & Technology GA 646DA UT WOS:000281515000006 PM 20306476 ER PT J AU Castro, A Cantrell, CL Hale, AL Duke, SO AF Castro, Amaya Cantrell, Charles L. Hale, Amber L. Duke, Stephen O. TI Phytotoxic Activity of Flavonoids from Dicranostyles ampla SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE flavonoids; Dicranostyles ampla; dihydromyricetin; myricitin-3-O-alpha-rhamnoside; phytotoxicity ID ANTIMICROBIAL CONSTITUENTS; CHEMOTAXONOMIC MARKERS; CUSCUTA-CHINENSIS; CONVOLVULACEAE; TRICOLORIN; GROWTH; HERBICIDES AB Crude extracts from over 16 species of plants from the family Convolvulaceae were evaluated for phytotoxic activity against Agrostis stolonifera (bentgrass) and Lactuca saliva (lettuce) at 1000 mu g/mL. Ethanol extracts of Dicranostyles ampla Ducke were among the most active of those species tested. Systematic bioassay-guided fractionation of the ethanol extract of the aerial parts from this species was performed to identify specifically the phytotoxic compounds. Two phytotoxic flavonoids, dihydromyricetin (1) and myricetin-3-O-alpha-rhamnoside or myricetrin (2), were found to be responsible for much of the activity of the extract as a whole in the A. stolonifera and L. sativa bioassay. In a Lemna paucicostata bioassay, 1 and 2 had no activity at 100 mu M. C1 [Castro, Amaya] IQAC CSIC, Barcelona 08034, Spain. [Cantrell, Charles L.; Hale, Amber L.; Duke, Stephen O.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. RP Castro, A (reprint author), IQAC CSIC, Jordi Girona 18-26, Barcelona 08034, Spain. EM amaya.castro@iqac.csic.es FU MCYT (Spanish Government) [BES-2005-9474] FX Short Stay Grant from MCYT (Spanish Government) to A.C. (Beca Predoctoral de Formacion de Personal Investigador BES-2005-9474) is gratefully acknowledged. We thank Robert Johnson for his technical assistance in the bioassays. NR 26 TC 1 Z9 1 U1 0 U2 10 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD AUG PY 2010 VL 5 IS 8 BP 1233 EP 1237 PG 5 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA 640TB UT WOS:000281074200017 PM 20839625 ER PT J AU Kim, DI Lee, SH Hong, JH Lillehoj, HS Park, HJ Rhie, SG Lee, GS AF Kim, Dae-Ik Lee, Sung-Hyen Hong, Joo-Heon Lillehoj, Hyun Soon Park, Hong-Ju Rhie, Seung-Gyo Lee, Gun-Soon TI The butanol fraction of Eclipta prostrata (Linn) increases the formation of brain acetylcholine and decreases oxidative stress in the brain and serum of cesarean-derived rats SO NUTRITION RESEARCH LA English DT Article DE Eclipta prostrata; Rat; Neurotransmitter; Antioxidant; Antidementia ID ALZHEIMERS-DISEASE; SUPEROXIDE-DISMUTASE; SENILE DEMENTIA; CHOLINERGIC DEFICITS; LIPID-PEROXIDATION; OXIDASE; NEUROTOXICITY; RADICALS; RELEASE; NEURONS AB Eclipta prostrata has been used as a traditional medicinal plant to prevent dementia and to enhance memory in Asia. Its potential as a nootropic and as an antioxidant have been reported in mice. We hypothesized that Eclipta may affect the formation of neurotransmitters and the inhibition of oxidative stress. Charles River cesarean-derived rats (male, 180 +/- 10 g) were fed experimental diets supplemented with 0 mg (control), 25 mg (E25), 50 mg (E50), or 100 mg (E100) of a freeze-dried butanol fraction of E prostrata per kilogram of diet for 6 weeks. The acetylcholine level was significantly increased by 9.6% and 12.1% in the brains of E50 and El 00 groups, respectively, as compared with the control group that was fed standard diet alone. The acetylcholine esterase activity was significantly increased by 13.1% and 19.7% in the brains of E50 and E100 groups, respectively, compared with the control group. Monoamine oxidase-B activity was significantly decreased by 10.5% in the brains of the E100 group, and the superoxide radical level was significantly reduced by 9.4% in the scrum of the E100 group compared with the control group. Superoxide dismutase activity was significantly increased by 9.6% and 11.6% in the serum of E50 and E100 groups, respectively, compared with the control group. These results clearly demonstrate the effects of E prostrata on the formation of acetylcholine in the brain and the inhibition of oxidative stress in the brain and serum of rats. These findings may have implications for preventing dementia and enhancing memory function in humans. Published by Elsevier Inc. C1 [Lee, Sung-Hyen] USDA ARS, Dept Agr, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Kim, Dae-Ik] Daegu Technopk Bio Ind Ctr, Taegu 704801, South Korea. [Lee, Sung-Hyen; Park, Hong-Ju] RDA, Natl Acad Agr Sci, Suwon 441853, South Korea. [Hong, Joo-Heon] Catholic Univ Daegu, Gyongsan 712702, South Korea. [Rhie, Seung-Gyo] Univ Suwon, Whasung 445760, Gyeonggi, South Korea. [Lee, Gun-Soon] RDA, Korea Natl Coll Agr & Fisheries, Bongdam Whasung 445760, Gyeonggi, South Korea. RP Lee, SH (reprint author), USDA ARS, Dept Agr, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM sunghyen.lee@ars.usda.gov FU Pukyong National University FX This work was partially supported by funds from the Pukyong National University. The authors thank Jin-Ho Choi, Hyo-Gwon Im, Mi-Hee Yu, and Stacy Torreyson for their contributions to this research. NR 51 TC 7 Z9 10 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD AUG PY 2010 VL 30 IS 8 BP 579 EP 584 DI 10.1016/j.nutres.2010.08.001 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 658DX UT WOS:000282471500010 PM 20851313 ER PT J AU Popkin, BM D'Anci, KE Rosenberg, IH AF Popkin, Barry M. D'Anci, Kristen E. Rosenberg, Irwin H. TI Water, hydration, and health SO NUTRITION REVIEWS LA English DT Review DE hydration; recommended daily intake; water; water adequacy; water intake; water measurement ID EXERCISE-INDUCED DEHYDRATION; GLUCOSE-SWEETENED BEVERAGES; NURSING-HOME RESIDENTS; DWELLING OLDER-ADULTS; FRUCTOSE CORN SYRUP; HEAT-STRESS; FLUID INTAKE; DRINKING-WATER; VOLUNTARY DEHYDRATION; COGNITIVE PERFORMANCE AB This review examines the current knowledge of water intake as it pertains to human health, including overall patterns of intake and some factors linked with intake, the complex mechanisms behind water homeostasis, and the effects of variation in water intake on health and energy intake, weight, and human performance and functioning. Water represents a critical nutrient, the absence of which will be lethal within days. Water's importance for the prevention of nutrition-related noncommunicable diseases has received more attention recently because of the shift toward consumption of large proportions of fluids as caloric beverages. Despite this focus, there are major gaps in knowledge related to the measurement of total fluid intake and hydration status at the population level; there are also few longer-term systematic interventions and no published randomized, controlled longer-term trials. This review provides suggestions for ways to examine water requirements and encourages more dialogue on this important topic. C1 [Popkin, Barry M.] Univ N Carolina, Carolina Populat Ctr, Dept Nutr, Chapel Hill, NC 27516 USA. [D'Anci, Kristen E.] Tufts Univ, Dept Psychol, Medford, MA 02155 USA. [Rosenberg, Irwin H.] Tufts Univ, Nutr & Neurocognit Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Popkin, BM (reprint author), Univ N Carolina, Carolina Populat Ctr, Dept Nutr, 123 W Franklin St, Chapel Hill, NC 27516 USA. EM popkin@unc.edu OI popkin, barry/0000-0001-9495-9324 FU Nestle Waters, Issy-les-Moulineaux, France; National Institute on Aging [5ROI AGI0436]; NIH [R01-CA109831, R01-CA121152] FX Funding. This work was supported by the Nestle Waters, Issy-les-Moulineaux, France, 5ROI AGI0436 from the National Institute on Aging Physical Frailty Program, and NIH R01-CA109831 and R01-CA121152. NR 147 TC 129 Z9 132 U1 13 U2 87 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD AUG PY 2010 VL 68 IS 8 BP 439 EP 458 DI 10.1111/j.1753-4887.2010.00304.x PG 20 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 628VP UT WOS:000280151900001 PM 20646222 ER PT J AU Dror, DK Allen, LH AF Dror, Daphna K. Allen, Lindsay H. TI Vitamin D inadequacy in pregnancy: biology, outcomes, and interventions SO NUTRITION REVIEWS LA English DT Review DE inadequacy; pregnancy; vitamin D ID GESTATIONAL DIABETES-MELLITUS; BREAST-FED INFANTS; D-DEFICIENCY; BONE-DENSITY; NUTRITIONAL RICKETS; CALCIUM HOMEOSTASIS; D SUPPLEMENTATION; ULTRAVIOLET-RADIATION; MULTIPLE-SCLEROSIS; WILLIAMS-SYNDROME AB A high prevalence of maternal vitamin D inadequacy during pregnancy and at delivery has been demonstrated in various ethnic populations living at different latitudes. Because placental transfer of 25(OH)D is the major source of vitamin D to the developing human fetus, there is growing concern about adverse health impacts that hypovitaminosis D during pregnancy may have on the mother as well as the offspring in utero, in infancy, and later in life. While there is lack of consensus regarding the optimal circulating 25(OH)D concentration in pregnancy, it is evident that prior levels used to establish intake recommendations and vitamin D content of prenatal vitamin supplements were too conservative. This review summarizes vitamin D metabolism in the perinatal period, examines evidence regarding outcomes of insufficiency in the mother and offspring, discusses risk factors and prevalence of insufficiency, and considers strategies for public health intervention. C1 [Dror, Daphna K.] ARS, USDA, Western Human Nutr Res Ctr, Allen Lab, Davis, CA 95616 USA. RP Dror, DK (reprint author), ARS, USDA, Western Human Nutr Res Ctr, Allen Lab, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM dkdror@ucdavis.edu NR 127 TC 69 Z9 73 U1 1 U2 16 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD AUG PY 2010 VL 68 IS 8 BP 465 EP 477 DI 10.1111/j.1753-4887.2010.00306.x PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 628VP UT WOS:000280151900003 PM 20646224 ER PT J AU Blumberg, J Heaney, RP Huncharek, M Scholl, T Stampfer, M Vieth, R Weaver, CM Zeisel, SH AF Blumberg, Jeffrey Heaney, Robert P. Huncharek, Michael Scholl, Theresa Stampfer, Meir Vieth, Reinhold Weaver, Connie M. Zeisel, Steven H. TI Evidence-based criteria in the nutritional context SO NUTRITION REVIEWS LA English DT Review DE benefit; evidence-based; nutritional policy; randomized clinical trials; risk ID RANDOMIZED CONTROLLED-TRIALS; VITAMIN-D SUPPLEMENTATION; SERVICES-TASK-FORCE; CALCIUM; METAANALYSIS; DISEASE; PREVENTION; LESSONS; HEALTH; FIELD AB During the last decade, approaches to evidence-based medicine, with its heavy reliance on the randomized clinical trial (RCT), have been adapted to nutrition science and policy. However, there are distinct differences between the evidence that can be obtained for the testing of drugs using RCTs and those needed for the development of nutrient requirements or dietary guidelines. Although RCTs present one approach toward understanding the efficacy of nutrient interventions, the innate complexities of nutrient actions and interactions cannot always be adequately addressed through any single research design. Because of the limitations inherent in RCTs, particularly of nutrients, it is suggested that nutrient policy decisions will have to be made using the totality of the available evidence. This may mean action at a level of certainty that is different from what would be needed in the evaluation of drug efficacy. Similarly, it is judged that the level of confidence needed in defining nutrient requirements or dietary recommendations to prevent disease can be different from that needed to make recommendations to treat disease. In brief, advancing evidence-based nutrition will depend upon research approaches that include RCTs but go beyond them. Also necessary to this advance is the assessing, in future human studies, of covariates such as biomarkers of exposure and response, and the archiving of samples for future evaluation by emerging technologies. C1 [Heaney, Robert P.] Creighton Univ, Omaha, NE 68131 USA. [Blumberg, Jeffrey] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Huncharek, Michael] St Louis Vet Adm Med Ctr, Div Radiat Oncol, St Louis, MO USA. [Scholl, Theresa] Univ Med & Dent New Jersey, Dept Obstet & Gynecol, Stratford, NJ USA. [Stampfer, Meir] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Stampfer, Meir] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Vieth, Reinhold] Univ Toronto, Dept Nutr Sci, Toronto, ON, Canada. [Vieth, Reinhold] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada. [Weaver, Connie M.] Purdue Univ, Dept Foods & Nutr, W Lafayette, IN 47907 USA. [Zeisel, Steven H.] Univ N Carolina, Dept Nutr, Chapel Hill, NC USA. [Zeisel, Steven H.] Univ N Carolina, Inst Nutr Res, Chapel Hill, NC USA. RP Heaney, RP (reprint author), Creighton Univ, 601 N 30th St,Suite 4841, Omaha, NE 68131 USA. EM rheaney@creighton.edu FU Creighton University; ConAgra Foods Inc., Omaha, Nebraska; Dairy Management Incorporated, Rosemont, Illinois; Council for Responsible Nutrition, Washington, DC; Preisland Foods; General Mills; Tate Lyle; Dairy Management Incorporated FX This paper is the product of an invited workshop convened at Creighton University, Omaha, Nebraska, USA, September 3-4, 2008. It was supported by Creighton University research funds and by unrestricted grants from ConAgra Foods Inc., Omaha, Nebraska; Dairy Management Incorporated, Rosemont, Illinois; and the Council for Responsible Nutrition, Washington, DC. Each author had a role in generating the concepts and preparing the manuscript.; Declaration of interest. CW has received research grants from Dairy Management Incorporated, Preisland Foods, General Mills, and Tate & Lyle. None of the other authors has relevant interests to declare. NR 36 TC 69 Z9 70 U1 0 U2 7 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD AUG PY 2010 VL 68 IS 8 BP 478 EP 484 DI 10.1111/j.1753-4887.2010.00307.x PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 628VP UT WOS:000280151900004 PM 20646225 ER PT J AU Liu, QA Tuo, WB Gao, HW Zhu, XQ AF Liu, Quan Tuo, Wenbin Gao, Hongwei Zhu, Xing-Quan TI MicroRNAs of parasites: current status and future perspectives SO PARASITOLOGY RESEARCH LA English DT Review ID SMALL INTERFERING RNAS; CAENORHABDITIS-ELEGANS; TRICHOMONAS-VAGINALIS; TRYPANOSOMA-BRUCEI; ENTAMOEBA-HISTOLYTICA; GIARDIA-LAMBLIA; GENE-EXPRESSION; C-ELEGANS; POSTTRANSCRIPTIONAL REGULATION; PROTOZOAN PARASITES AB MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs regulating gene expression in eukaryotes at the post-transcriptional level. The complex life cycles of parasites may require the ability to respond to environmental and developmental signals through miRNA-mediated gene expression. Over the past 17 years, thousands of miRNAs have been identified in the nematode Caenorhabditis elegans and other parasites. Here, we review the current status and potential functions of miRNAs in protozoan, helminths, and arthropods, and propose some perspectives for future studies. C1 [Liu, Quan; Gao, Hongwei] AMMS Key Lab Jilin Prov Zoonosis Prevent & Contro, Inst Mil Vet, Changchun 130062, Jilin, Peoples R China. [Tuo, Wenbin] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Zhu, Xing-Quan] CAAS, Dept Parasitol, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China. RP Gao, HW (reprint author), AMMS Key Lab Jilin Prov Zoonosis Prevent & Contro, Inst Mil Vet, 1068 Qinglong Rd, Changchun 130062, Jilin, Peoples R China. EM ccltdz@sohu.com; xingquanzh@scau.edu.cn FU National Basic Research Program of China [2007CB513104]; National Natural Science Foundation of China [30972178]; National Special Research Program [200803017]; Program for Changjiang Scholars and Innovative Research Team in University [IRT0723]; National Key Technology R&D Program of China [2010BAD04B01]; Academy of Military Medical Sciences [YCX0901] FX This work is supported, in part, by grants from the National Basic Research Program of China (Grant no. 2007CB513104), National Natural Science Foundation of China (Grant no. 30972178), the National Special Research Program for Non-Profit Trades (Agriculture; Grant no. 200803017), the Program for Changjiang Scholars and Innovative Research Team in University (Grant no. IRT0723), National Key Technology R&D Program of China (Grant no. 2010BAD04B01), and "Gold Idea" Foundation of Institute of Military Veterinary, Academy of Military Medical Sciences (Grant no. YCX0901). NR 68 TC 26 Z9 28 U1 2 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 J9 PARASITOL RES JI Parasitol. Res. PD AUG PY 2010 VL 107 IS 3 BP 501 EP 507 DI 10.1007/s00436-010-1927-6 PG 7 WC Parasitology SC Parasitology GA 634ER UT WOS:000280562900001 PM 20532562 ER PT J AU Anderson, CM Uthus, EO AF Anderson, C. M. Uthus, E. O. TI PLACENTAL EPIGENETIC ALTERATIONS AND VITAMIN D SIGNALING IN PREECLAMPSIA SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Aspen Perinatal Biology Conference 2010 CY AUG 28-31, 2010 CL Aspen, CO C1 [Anderson, C. M.] Univ N Dakota, Coll Nursing, Grand Forks, ND 58201 USA. [Uthus, E. O.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. RI Anderson, Cindy/I-3900-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 W CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD AUG PY 2010 VL 68 IS 2 BP 174 EP 174 PG 1 WC Pediatrics SC Pediatrics GA 629PJ UT WOS:000280211900018 ER PT J AU Weindorf, DC Bakr, N Zhu, Y Haggard, B Johnson, S Daigle, J AF Weindorf, D. C. Bakr, N. Zhu, Y. Haggard, B. Johnson, S. Daigle, J. TI Characterization of Placic Horizons in Ironstone Soils of Louisiana, USA SO PEDOSPHERE LA English DT Article DE ortstein; pedon; petroferric contact; Petrudepts ID FOREST; IRON; INCEPTISOLS; HEMATITE; GOETHITE; GENESIS AB Placic horizons, defined as thin, wavy, hardened layers of iron and organic matter, are rare within the United States, occurring only in Washington, Alaska, and Hawaii. While ironstone is common in many soils of the southeastern United States, it is not known to contain appreciable organic matter. As a pilot study evaluating the justification for a larger study on ironstone in Louisiana, a 40 m lateral exposure with suspected placic horizons was evaluated in Vernon Parish, Louisiana. Results of laboratory analysis show elevated levels of iron and organic matter in the suspect horizons that meet the criteria of placic horizons as defined by the Soil Survey Staff. Based on the results of this study, additional evaluation of multiple pedons with similar features is warranted. Should additional pedons demonstrate similar properties, a new great group of 'Petrudepts' would be needed to describe both the placic horizons in the pedon and the udic moisture regime in which they occur. C1 [Weindorf, D. C.; Bakr, N.; Zhu, Y.; Haggard, B.; Johnson, S.] Louisiana State Univ Agr Ctr, Baton Rouge, LA 70803 USA. [Daigle, J.] Nat Resources Conservat Serv, USDA, Alexandria, LA 71302 USA. RP Weindorf, DC (reprint author), Louisiana State Univ Agr Ctr, 307 MB Sturgis Hall, Baton Rouge, LA 70803 USA. EM dweindorf@agcenter.lsu.edu RI Zhu, Yuanda/A-1877-2010; Weindorf, David/C-4322-2012; Bakr, Noura/P-7650-2015 OI Bakr, Noura/0000-0001-6935-1560 FU LSU AgCenter, USA FX Supported by the LSU AgCenter, USA. NR 26 TC 3 Z9 3 U1 1 U2 3 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 J9 PEDOSPHERE JI Pedosphere PD AUG PY 2010 VL 20 IS 4 BP 409 EP 418 PG 10 WC Soil Science SC Agriculture GA 631AL UT WOS:000280318600001 ER PT J AU Dezengrini, R da Silva, SC Weiss, M de Oliveira, MS Traesel, CK Weiblen, R Flores, EF AF Dezengrini, Renata da Silva, Sara C. Weiss, Marcelo de Oliveira, Mauro S. Traesel, Carolina K. Weiblen, Rudi Flores, Eduardo F. TI Effects of Foscarnet on the infection by bovine herpesviruses 1 and 5 in rabbits SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Antivirals; BoHV-1; BoHV-5; PFA; rabbits ID HERPES-SIMPLEX-VIRUS; IN-VITRO; ACYCLOVIR OINTMENT; TYPE-1 INFECTION; PHOSPHONOFORMATE; MENINGOENCEPHALITIS; GANCICLOVIR; CIDOFOVIR; BHV-5; MODEL AB Dezengrini R., Silva S. C., Weiss M., Oliveira M. S., Traesel C. K., Weiblen R. & Flores E. F. 2010. [Effects of Foscarnet on the infection by bovine herpesviruses 1 and 5 in rabbits.] Efeitos do Foscarnet sobre a infeccao pelos herpesvirus bovino tipos 1 e 5 em coelhos. Pesquisa Veterinaria Brasileira 30(8): 623-630. Setor de Virologia, Departamento de Medicina Veterinaria Preventiva, Centro de Ciencias Rurais, Universidade Federal de Santa Maria, Av, Roraima 1000, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: eduardofurtadoflores@gmail.com The activity of Foscarnet (PFA) against three bovine herpesviruses (BoHVs) was previously demonstrated in cell culture. Herein we evaluated the effects of PFA on the infection and disease by BoHV-1 and BoHV-5 in a rabbit model. Rabbits inoculated with BoHV-5 in the conjunctival sac (IC) and treated with PFA (100 mg/kg/day) from day 1 to 17 post-inoculation (pi) shed less virus between days 2 and 6 pi comparing to untreated controls; this difference was significant at day 3 pi [F(9,108) = 2,23; P<0.03]. The morbidity and mortality rates of rabbits inoculated with BoHV-5 IC or intranasally (IN) were also significantly reduced in PFA-treated rabbits (50%; 11/22) comparing to untreated controls (95.4%; 21/22) (P<0.0008). In rabbits inoculated IC with BoHV-1, a reduction in virus shedding was observed in PFA-treated animals between days 1 and 4 pi; 6 and 7 pi. In addition, PFA-treated rabbits presented a longer incubation period and a shorter clinical course comparing to untreated controls (P<0.005 and P<0.04, respectively). The frequency and severity of ocular signs were also reduced in the PFA-treated group. These results demonstrate that PFA is effective against BoHV-1 and BoHV-5 in vivo and open the way towards its use in experimental therapy of herpetic infections in domestic animals. C1 [Weiblen, Rudi; Flores, Eduardo F.] Univ Fed Santa Maria, Setor Virol, Dept Med Vet Prevent, Ctr Ciencias Rurais, Santa Maria, RS, Brazil. [Dezengrini, Renata; da Silva, Sara C.; Weiss, Marcelo; Traesel, Carolina K.] Univ Fed Santa Maria, Programa Posgrad Med Vet, BR-97105900 Santa Maria, RS, Brazil. [Weiss, Marcelo] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. [de Oliveira, Mauro S.] Univ Fed Santa Maria, Lab Neurotoxicidade & Psicofarmacol, BR-97105900 Santa Maria, RS, Brazil. RP Flores, EF (reprint author), Univ Fed Santa Maria, Setor Virol, Dept Med Vet Prevent, Ctr Ciencias Rurais, Santa Maria, RS, Brazil. EM eduardofurtadoflores@gmail.com RI Weiss, Marcelo/I-1274-2012; Traesel, Carolina/A-2777-2013; Weiblen, Rudi/H-7003-2014; flores, eduardo/H-5125-2016 OI Weiss, Marcelo/0000-0001-7902-3210; Weiblen, Rudi/0000-0002-1737-9817; NR 30 TC 0 Z9 0 U1 0 U2 1 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X J9 PESQUISA VET BRASIL JI Pesqui. Vet. Bras. PD AUG PY 2010 VL 30 IS 8 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 658WM UT WOS:000282524300003 ER PT J AU Knight, AL AF Knight, Alan L. TI Cross-resistance between azinphos-methyl and acetamiprid in populations of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), from Washington State SO PEST MANAGEMENT SCIENCE LA English DT Article DE chloronicotinyl; organophosphate; bioassays; adults; neonates ID INSECTICIDE RESISTANCE; CENTRAL CHILE; MECHANISMS; ORCHARDS; SPINOSAD; DELTAMETHRIN; LARVAE; SUSCEPTIBILITY; DIFLUBENZURON; GRANULOVIRUS AB BACKGROUND: Codling moth, Cydia pomonella (L.), has been intensely managed with the organophosphate insecticide azinphos-methyl for 50 years, and populations have developed resistance. New management programs have been developed and implemented that rely more heavily on other classes of insecticides. A prerequisite for developing effective resistance management strategies for these compounds is to establish their current levels of effectiveness. Adult and neonate larval assays were conducted to assess the response of field-collected codling moth populations from apple in Washington State. RESULTS: Male codling moth populations exhibited a range of responses to a discriminating concentration of azinphosmethyl in a survey of 20 populations. Populations from certified organic orchards were more susceptible than those from conventional orchards. Mean fecundity was inversely related to azinphos-methyl tolerance. Male responses to azinphos-methyl and acetamiprid varied significantly among populations and were correlated. The residual effectiveness of field applications of both insecticides varied significantly against neonate larvae. Neonate bioassays with insecticide-dipped fruit found significant differences among populations with azinphos-methyl, acetamiprid, methoxyfenozide and spinosad, but not with esfenvalerate. CONCLUSION: These results support a concern that alternation of insecticides with different modes of action may not be a sufficient strategy to avoid the evolution of broad-spectrum insecticide resistance by codling moth. Published 2010 by John Wiley & Sons, Ltd. C1 [Knight, Alan L.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. RP Knight, AL (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM Alan.Knight@ars.usda.gov NR 61 TC 17 Z9 17 U1 0 U2 8 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD AUG PY 2010 VL 66 IS 8 BP 865 EP 874 DI 10.1002/ps.1955 PG 10 WC Agronomy; Entomology SC Agriculture; Entomology GA 627VF UT WOS:000280071000009 PM 20603873 ER PT J AU Hanson, BD Gerik, JS Schneider, SM AF Hanson, Bradley D. Gerik, James S. Schneider, Sally M. TI Effects of reduced-rate methyl bromide applications under conventional and virtually impermeable plastic film in perennial crop field nurseries SO PEST MANAGEMENT SCIENCE LA English DT Article DE bare-root; methyl bromide; nursery; soil fumigation; virtually impermeable film ID ALTERNATIVES AB BACKGROUND: Producers of perennial crop nursery stock in California use preplant soil fumigation to meet state phytosanitary requirements. Although methyl bromide (MB) has been phased out in many agricultural industries, it is still the preferred treatment in the perennial nursery industry and is used under Critical Use Exemptions and Quarantine/Preshipment provisions of the Montreal Protocol. The present research was conducted to evaluate reduced-rate MB applications sealed with conventional and low-permeability plastic films compared with the primary alternative material. RESULTS: Reduced rates (100-260 kg ha(-1)) of MB applied in combination with chloropicrin (Pic) and sealed with a low-permeability plastic film provided weed and nematode control similar to the industry standard rate of 392 kg ha-1 MB: Pic (98 : 2) sealed with high-density polyethylene (HDPE) film. However, the primary alternative chemical, 1,3-dichloropropene (1,3-D), tended to provide slightly lower pest control even on sites with relatively low plant parasitic nematode, soil-borne pathogen and weed pest pressure. CONCLUSION: If California regulations change to allow the use of low-permeability films in broadcast fumigant applications, the results of this research suggest that reduced rates of MB in perennial crop nurseries could serve as a bridge strategy until more technically, economically and environmentally acceptable alternatives are developed. Published 2010 by John Wiley & Sons, Ltd. C1 [Hanson, Bradley D.; Gerik, James S.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. [Schneider, Sally M.] ARS, USDA, Beltsville, MD USA. RP Hanson, BD (reprint author), Univ Calif Davis, Dept Plant Sci, Mail Stop 4,1 Shields Ave, Davis, CA 95616 USA. EM bhanson@ucdavis.edu NR 21 TC 12 Z9 12 U1 1 U2 9 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD AUG PY 2010 VL 66 IS 8 BP 892 EP 899 DI 10.1002/ps.1959 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA 627VF UT WOS:000280071000013 PM 20602523 ER PT J AU Dowd, PF Johnson, ET Pinkerton, TS AF Dowd, Patrick F. Johnson, Eric T. Pinkerton, T. Scott TI Identification and properties of insect resistance-associated maize anionic peroxidases SO PHYTOCHEMISTRY LA English DT Article DE Zea mays L.; Poaceae; Maize; Ferulic acid; p-Coumaric acid; Helicoverpa; Spodoptera ID ZEA-MAYS L; HORSERADISH-PEROXIDASE; LEAF RESISTANCE; ACID; PLANTS; RESPONSES; VECTORS; DISEASE; SILENT; ENZYME AB Previous studies with transgenic plants have indicated a tobacco anionic peroxidase can confer enhanced resistance to a variety of insects when expressed in different plant species. Tissue that expresses high levels of this enzyme often browns rapidly when damaged. Maize roots damaged under sterile conditions browned and had an anionic peroxidase induced. When introduced biolistically, maize callus transformants expressing a maize peroxidase gene with a predicted isoelectric point of ca. 5.1 produced browner callus compared to a corresponding beta-glucuronidase (GUS) transformant as callus aged. Higher production of only one isozyme of ca. pl 4.5 was noted. When the callus was fed to two maize pest caterpillar species, growth rates were slower (as reflected by weights) relative to the GUS callus. Based on examination of published information and electrophoretic properties, this gene appears to code for Px11, a peroxidase isozyme that is primarily produced in root tissue and callus. When sequence of the gene in several inbreds was examined, coding variations were noted, and abilities to utilize ferulic and p-coumaric acids differed. These coding differences may influence the ability of corresponding forms of the peroxidase to promote resistance. In addition to potential use in marker assisted breeding, enhanced expression of this anionic peroxidase through breeding or genetic engineering may lead to enhanced insect or disease resistance. Published by Elsevier Ltd. C1 [Dowd, Patrick F.; Johnson, Eric T.; Pinkerton, T. Scott] ARS, Crop BioProtect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Dowd, PF (reprint author), ARS, Crop BioProtect Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Patrick.Dowd@ars.usda.gov OI Johnson, Eric/0000-0002-1665-6229 FU US Department of Agriculture [583K95-6-474]; Pioneer Hi-Bred, International [583K95-6-474] FX We thank N. Deppe, D.A. Lee, A. Morgan, K. Shopinski, J. Steele and R. Stessman, (all of USDA, Peoria, IL) for technical assistance, M.J. Muhitch (Rochester University) for providing the pAHC25 plasmid, Iowa State University for providing seed, Pioneer Hi-Bred, a DuPont Company, for providing the cDNA clone (C.R. Simmons), P.S. Chourey (U. Florida) for providing the BMS cell culture, C. Skory (USDA, Peoria, IL) and M.J. Muhitch for technical advise to PFD, M.J. Muhitch, P.J. Slininger, F.E. Vega and anonymous reviewers for comments on prior drafts of this manuscript. Some the data generated for presentation in this manuscript was facilitated by Cooperative Research and Development Agreement #583K95-6-474 between the US Department of Agriculture and Pioneer Hi-Bred, International. NR 44 TC 12 Z9 12 U1 3 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9422 J9 PHYTOCHEMISTRY JI Phytochemistry PD AUG PY 2010 VL 71 IS 11-12 BP 1289 EP 1297 DI 10.1016/j.phytochem.2010.05.001 PG 9 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 631BH UT WOS:000280321000010 PM 20553698 ER PT J AU Kang, S Mansfield, MA Park, B Geiser, DM Ivors, KL Coffey, MD Grunwald, NJ Martin, FN Levesque, CA Blair, JE AF Kang, Seogchan Mansfield, Michele A. Park, Bongsoo Geiser, David M. Ivors, Kelly L. Coffey, Michael D. Gruenwald, Niklaus J. Martin, Frank N. Levesque, C. Andre Blair, Jaime E. TI The Promise and Pitfalls of Sequence-Based Identification of Plant-Pathogenic Fungi and Oomycetes SO PHYTOPATHOLOGY LA English DT Article DE culture collection; database; diagnosis; DNA barcode; phylogeny; taxonomy ID SOLANI SPECIES COMPLEX; MOLECULAR PHYLOGENY; DNA-SEQUENCES; PHYTOPHTHORA; FUSARIUM; DATABASE; LIFE; GENBANK; TREE; RECOGNITION AB Sequences of selected marker loci have been widely used for the identification of specific pathogens and the development of sequence-based diagnostic methods. Although such approaches offer several advantages over traditional culture-based methods for pathogen diagnosis and identification, they have their own pitfalls. These include erroneous and incomplete data in reference databases, poor or oversimplified interpretation of search results, and problems associated with defining species boundaries. In this letter, we outline the potential benefits and drawbacks of using sequence data for identification and taxonomic deductions of plant-pathogenic fungi and oomycetes, using Phytophthora as a primary example. We also discuss potential remedies for these pitfalls and address why coordinated community efforts are essential to make such remedies more efficient and robust. C1 [Kang, Seogchan; Mansfield, Michele A.; Park, Bongsoo; Geiser, David M.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. [Ivors, Kelly L.] N Carolina State Univ, Mt Hort Crops Res & Extens Ctr, Mills River, NC 28759 USA. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Gruenwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. [Martin, Frank N.] USDA ARS, Salinas, CA 93906 USA. [Levesque, C. Andre] Agr & Agri Food Canada, Ottawa, ON K1A 0C6, Canada. [Blair, Jaime E.] Franklin & Marshall Coll, Dept Biol, Lancaster, PA 17604 USA. RP Kang, S (reprint author), Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. EM sxk55@psu.edu RI Grunwald, Niklaus/K-6041-2013; Geiser, David/J-9950-2013 OI Grunwald, Niklaus/0000-0003-1656-7602; FU USDA [2005-35605-15393, 2008-55605-18773]; Canadian Barcode of Life Network FX We acknowledge the USDA-AFRI Plant Biosecurity Program for its support for the Phytophthora Database project (2005-35605-15393 and 2008-55605-18773). The work of C. A. Levesque is partly supported by the Canadian Barcode of Life Network. NR 51 TC 34 Z9 34 U1 1 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2010 VL 100 IS 8 BP 732 EP 737 DI 10.1094/PHYTO-100-8-0732 PG 6 WC Plant Sciences SC Plant Sciences GA 628RX UT WOS:000280139600001 PM 20626276 ER PT J AU Hartung, JS Paul, C Achor, D Brlansky, RH AF Hartung, John S. Paul, Cristina Achor, Diann Brlansky, R. H. TI Colonization of Dodder, Cuscuta indecora, by 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' SO PHYTOPATHOLOGY LA English DT Article ID SAO-PAULO STATE; REAL-TIME PCR; GREENING DISEASE; PLANT-VIRUSES; SWEET ORANGE; CITRUS; BRAZIL; TRANSMISSION; 1ST-REPORT; BACTERIUM AB Huanglongbing, or citrus greening, threatens the global citrus industry. The presumptive pathogens, 'Candidatus Liberibacter asiaticus' and 'Ca. L. americanus' can be transferred from citrus to more easily studied experimental hosts by using holoparasitic dodder plants. However, the interaction between 'Candidatus Liberibacter' spp. and the dodder has not been studied. We combined quantitative polymerase chain reaction with electron microscopy to show that only 65% of tendrils of Cuscuta indecora grown on 'Ca. Liberibacter spp.-infected host plants had detectable levels of the pathogen. Among tendrils that were colonized by Liberibacter in at least one 2 cm segment, most were not colonized in all segments. Furthermore, the estimated population levels of the pathogen present in serial 2 cm segments of dodder tendrils varied widely and without any consistent pattern. Thus, there was generally not a concentration gradient of the pathogen from the source plant towards the recipient and populations of the pathogen were sometimes found in the distal segments of the dodder plant but not in the proximal or middle segments. Populations of the pathogens ranged from 2 x 10(2) to 3.0 x 10(8) cells per 2 cm segment. On a fresh weight basis, populations as high as 1.4 x 10(10) cells per g of tissue were observed demonstrating that 'Ca. Liberibacter' spp. multiplies well in Cuscuta indecora. However, 55% of individual stem segments did not contain detectable levels of the pathogen, consistent with a pattern of nonuniform colonization similar to that observed in the much more anatomically complex citrus tree. Colonization of dodder by the pathogen is also nonuniform at the ultrastructural level, with adjacent phloem vessel elements being completely full of the pathogen or free of the pathogen. We also observed bacteria in the phloem vessels that belonged to two distinct size classes based on the diameters of cross sections of cells. In other sections from the same tendrils we observed single bacterial cells that were apparently in the process of differentiating between the large and round forms to the long and thin forms (or vice versa). The process controlling this morphological differentiation of the pathogen is not known. The highly reduced and simplified anatomy of the dodder plant as well as its rapid growth rate compared with citrus, and the ability of the plant to support multiplication of the pathogen to high levels, makes it an interesting host plant for further studies of host pathogen interactions. C1 [Hartung, John S.; Paul, Cristina] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. [Achor, Diann; Brlansky, R. H.] Univ Florida, Dept Plant Pathol, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. RP Hartung, JS (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. EM john.hartung@ars.usda.gov NR 32 TC 17 Z9 18 U1 6 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2010 VL 100 IS 8 BP 756 EP 762 DI 10.1094/PHYTO-100-8-0756 PG 7 WC Plant Sciences SC Plant Sciences GA 628RX UT WOS:000280139600004 PM 20626279 ER PT J AU Spokas, KA Baker, JM Reicosky, DC AF Spokas, Kurt A. Baker, John M. Reicosky, Donald C. TI Ethylene: potential key for biochar amendment impacts SO PLANT AND SOIL LA English DT Article DE Biochar; Black carbon; Charcoal; Greenhouse gas ID ENCAPSULATED CALCIUM CARBIDE; SOIL; INHIBITION; CHARCOAL; FOREST; CARBON; PYROLYSIS; EMISSIONS; GROWTH; YIELD AB Significant increases in root density, crop growth and productivity have been observed following soil additions of biochar, which is a solid product from the pyrolysis of biomass. In addition, alterations in the soil microbial dynamics have been observed following biochar amendments, with decreased carbon dioxide (CO(2)) respiration, suppression of methane (CH(4)) oxidation and reduction of nitrous oxide (N(2)O) production. However, there has not been a full elucidation of the mechanisms behind these effects. Here we show data on ethylene production that was observed from biochar and biochar-amended soil. Ethylene is an important plant hormone as well as an inhibitor for soil microbial processes. Our current hypothesis is that the ethylene is biochar derived, with a majority of biochars exhibiting ethylene production even without soil or microbial inoculums. There was increased ethylene production from non-sterile compared to sterile soil (215%), indicating a role of soil microbes in the observed ethylene production. Production varied with different biomass sources and production conditions. These observations provide a tantalizing insight into a potential mechanism behind the biochar effects observed, particularly in light of the important role ethylene plays in plant and microbial processes. C1 [Spokas, Kurt A.; Baker, John M.] USDA ARS, St Paul, MN 55108 USA. [Spokas, Kurt A.; Baker, John M.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Reicosky, Donald C.] USDA ARS, Morris, MN USA. RP Spokas, KA (reprint author), USDA ARS, 1991 Upper Buford Circle,439 Borlaug Hall, St Paul, MN 55108 USA. EM kurt.spokas@ars.usda.gov RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 NR 42 TC 94 Z9 103 U1 5 U2 134 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X J9 PLANT SOIL JI Plant Soil PD AUG PY 2010 VL 333 IS 1-2 BP 443 EP 452 DI 10.1007/s11104-010-0359-5 PG 10 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA 628BO UT WOS:000280089400035 ER PT J AU Lehman, RM Osborne, SL Prischmann-Voldseth, DA Rosentrater, KA AF Lehman, R. Michael Osborne, Shannon L. Prischmann-Voldseth, Deirdre A. Rosentrater, Kurt A. TI Insect-damaged corn stalks decompose at rates similar to Bt-protected, non-damaged corn stalks SO PLANT AND SOIL LA English DT Article DE Bt; Corn; Decomposition; GM; Maize; Zea mays L. ID BACILLUS-THURINGIENSIS; MAIZE; RESIDUE; FIELD; SOIL; GRAIN; MINERALIZATION; IMPACT; SILAGE; CARBON AB The relative decomposability of corn (Zea mays L.) residues from insect (Bt)-protected hybrids and conventional hybrids cultivated under insect pressure was investigated in two studies. Above-ground biomass, residue macromolecular composition, and stalk physical strength were also measured. In the first decomposition study, chopped residues (stalks and leaves) were used from a corn rootworm-protected (Cry3Bb1) hybrid and its non-Bt near isoline that were grown in replicated plots infested with corn rootworms (Diabrotica spp.). In the second study, residue (intact stalk sections) was used from three European corn borer (ECB, Ostrinia nubilalis Hubner)-resistant (Cry1Ab) hybrids representing different seed manufacturer/maturity date series, their non-Bt near isolines, two Cry3Bb1-protected isolines, and three additional conventional hybrids, all cultivated in replicated plots under conditions of elevated ECB pressure. In both studies, insect-resistant residues decomposed at rates similar to their non-protected near isolines. No evidence was found that insect-protected hybrids produced more above-ground biomass or had distinct residue composition. While some measures of mechanical stalk strength indicated that ECB-damaged stalks were not as stiff as protected stalks, these physical differences did not translate into differences in residue decomposition. We conclude that while individual hybrids may vary in their production of biomass, residue composition or residue decomposability, these characteristics do not systematically vary with the presence of the Bt gene conferring insect resistance, even under conditions of insect pressure. C1 [Lehman, R. Michael; Osborne, Shannon L.; Rosentrater, Kurt A.] USDA ARS N Cent Agr Res Lab, Brookings, SD 57006 USA. [Prischmann-Voldseth, Deirdre A.] N Dakota State Univ, Dept Entomol, Fargo, ND 58105 USA. RP Lehman, RM (reprint author), USDA ARS N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM michael.lehman@ars.usda.gov OI Lehman, Michael/0000-0002-3391-3178; Rosentrater, Kurt/0000-0003-0131-7037 NR 34 TC 9 Z9 9 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X J9 PLANT SOIL JI Plant Soil PD AUG PY 2010 VL 333 IS 1-2 BP 481 EP 490 DI 10.1007/s11104-010-0364-8 PG 10 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA 628BO UT WOS:000280089400038 ER PT J AU Li, R Qu, R Bruneau, AH Livingston, DP AF Li, R. Qu, R. Bruneau, A. H. Livingston, D. P. TI Selection for freezing tolerance in St. Augustinegrass through somaclonal variation and germplasm evaluation SO PLANT BREEDING LA English DT Article DE Stenotaphrum secundatum; freezing tolerance; germplasm; tissue culture ID COLD-ACCLIMATION; LOLIUM-PERENNE; CARBOHYDRATE; BERMUDAGRASS; HARDINESS; GENES AB P>St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is the least cold-hardy turfgrass species. Development of freezing-tolerant St. Augustinegrass cultivars would greatly benefit home owners in many southern states of the US. Towards this breeding goal, 7800 plants regenerated through tissue culture and 36 germplasm accessions were screened for improved freezing tolerance. Among the conditions tested, 1 week at 13 degrees C followed by another week at 3 degrees C, then freezing at -3 to -5 degrees C for 3 h, was found to be suitable to distinguish genotypes in freezing tests. The experiments revealed that germplasm accession Elm4 was significantly more freezing-tolerant under a controlled environment than 'Raleigh', the current commercially available, most freezing-tolerant cultivar. In addition, out of 7800 regenerated plants from tissue culture, somaclonal variant SVC3 showed significantly more freezing-tolerant than its parent 'Raleigh'. C1 [Livingston, D. P.] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. [Li, R.; Qu, R.; Bruneau, A. H.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Livingston, DP (reprint author), N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. EM dpl@unity.ncsu.edu FU NCSU Turfgrass Environmental Research and Education Center FX We thank Drs M. Fraser and W. Hanna for their helpful discussion and input, to Drs C. Peacock, R. Nagata and all colleagues, extension agents, and turf industry supporters who helped us in germplasm collections. We also thank Drs J. Thomas, J. Shurtleff, C. Saravitz of the NCSU Phytotron for assistance in using the facility. We are grateful to Dr C. Brownie for discussion and assistance in statistical analysis, to Dr P. Murphy and N. Robertson for greenhouse facility, and to Dr P. Premakumar for technical help in conducting the freezing tests. We are also thankful to B. Whaley, A. Johnson, T. Lambert and B. Erickson for assistance in the field trials. This project was funded by the NCSU Turfgrass Environmental Research and Education Center. NR 26 TC 7 Z9 9 U1 0 U2 7 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD AUG PY 2010 VL 129 IS 4 BP 417 EP 421 DI 10.1111/j.1439-0523.2009.01743.x PG 5 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 635EH UT WOS:000280637900011 ER PT J AU Bastow, R Beynon, J Estelle, M Friesner, J Grotewold, E Lavagi, I Lindsey, K Meyers, B Provart, N Benfey, P Birney, E Braun, P Brendel, V Buell, R Caccamo, M Carrington, J Cherry, M Ecker, J Eppig, J Forster, M Gutierrez, R Hilson, P Huala, E Katari, M Kersey, P Kudla, J Ma, H Matsui, M Matthews, K May, S Mayer, K Millar, A Millar, H Mjolsness, E Mockler, T Nikolau, B Nordborg, M Rawlings, C Schofield, P Schoof, H Schroeder, JI Sen, TZ Stanzione, D Town, C Toyoda, T Vision, T Walsh, S Wang, XJ Ware, D Weckwerth, W Yang, WC AF Bastow, Ruth Beynon, Jim Estelle, Mark Friesner, Joanna Grotewold, Erich Lavagi, Irene Lindsey, Keith Meyers, Blake Provart, Nicholas Benfey, Philip Birney, Ewan Braun, Pascal Brendel, Volker Buell, Robin Caccamo, Mario Carrington, Jim Cherry, Mike Ecker, Joseph Eppig, Janan Forster, Mark Gutierrez, Rodrigo Hilson, Pierre Huala, Eva Katari, Manpreet Kersey, Paul Kudla, Joerg Ma, Hong Matsui, Minami Matthews, Kathy May, Sean Mayer, Klaus Millar, Andrew Millar, Harvey Mjolsness, Eric Mockler, Todd Nikolau, Basil Nordborg, Magnus Rawlings, Chris Schofield, Paul Schoof, Heiko Schroeder, Julian I. Sen, Taner Z. Stanzione, Dan Town, Chris Toyoda, Tetsuro Vision, Todd Walsh, Sean Wang, Xiujie Ware, Doreen Weckwerth, Wolfram Yang, Weicai CA Int Arabidopsis Informatics Cons TI An International Bioinformatics Infrastructure to Underpin the Arabidopsis Community SO PLANT CELL LA English DT Editorial Material AB The future bioinformatics needs of the Arabidopsis community as well as those of other scientific communities that depend on Arabidopsis resources were discussed at a pair of recent meetings held by the Multinational Arabidopsis Steering Committee and the North American Arabidopsis Steering Committee. There are extensive tools and resources for information storage, curation, and retrieval of Arabidopsis data that have been developed over recent years primarily through the activities of The Arabidopsis Information Resource, the Nottingham Arabidopsis Stock Centre, and the Arabidopsis Biological Resource Center, among others. However, the rapid expansion in many data types, the international basis of the Arabidopsis community, and changing priorities of the funding agencies all suggest the need for changes in the way informatics infrastructure is developed and maintained. We propose that there is a need for a single core resource that is integrated into a larger international consortium of investigators. We envision this to consist of a distributed system of data, tools, and resources, accessed via a single information portal and funded by a variety of sources, under shared international management of an International Arabidopsis Informatics Consortium (IAIC). This article outlines the proposal for the development, management, operations, and continued funding for the IAIC. C1 [Beynon, Jim] Univ Warwick, Coventry CV4 7AL, W Midlands, England. [Estelle, Mark; Schroeder, Julian I.] Univ Calif San Diego, San Diego, CA 92103 USA. [Grotewold, Erich; Carrington, Jim; Mockler, Todd] Ohio State Univ, Columbus, OH 43210 USA. [Lindsey, Keith] Univ Durham, Durham DH1 3HP, England. [Meyers, Blake] Univ Delaware, Newark, DE 19716 USA. [Provart, Nicholas] Univ Toronto, Toronto, ON M5S 1A1, Canada. [Benfey, Philip] Duke Univ, Durham, NC 27706 USA. [Braun, Pascal] Dana Farber Canc Inst, Boston, MA USA. [Braun, Pascal] Harvard Univ, Sch Med, Cambridge, MA 02138 USA. [Sen, Taner Z.] Iowa State Univ, USDA, ARS, Res Serv, Ames, IA 50011 USA. [Buell, Robin] Michigan State Univ, E Lansing, MI 48824 USA. [Cherry, Mike] Stanford Univ, Stanford, CA 94305 USA. [Gutierrez, Rodrigo] Catholic Univ Chile, Santiago, Chile. [Hilson, Pierre] VIB Ghent, Ghent, Belgium. [Katari, Manpreet] NYU, New York, NY 10003 USA. [Kudla, Joerg] Univ Munster, D-4400 Munster, Germany. [Ma, Hong] Fudan Univ, Shanghai, Peoples R China. [Ma, Hong] Penn State Univ, University Pk, PA 16802 USA. [Matsui, Minami; Toyoda, Tetsuro] RIKEN, Wako, Saitama, Japan. [Matthews, Kathy] Indiana Univ, Bloomington, IN 47405 USA. [May, Sean] Univ Nottingham, Nottingham NG7 2RD, England. [Millar, Andrew] Univ Edinburgh, Edinburgh EH8 9YL, Midlothian, Scotland. [Millar, Harvey] Univ Western Australia, Nedlands, WA 6009, Australia. [Mjolsness, Eric] Univ Calif Irvine, Irvine, CA USA. [Schofield, Paul] Univ Cambridge, Cambridge CB2 1TN, England. [Sen, Taner Z.] Iowa State Univ, MaizeGDB, Ames, IA 50011 USA. [Stanzione, Dan] Univ Texas Austin, Austin, TX 78712 USA. [Walsh, Sean] ETH, Zurich, Switzerland. [Yang, Weicai] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100864, Peoples R China. [Ware, Doreen] ARS, USDA, Cold Spring Harbor Lab, Washington, DC USA. [Weckwerth, Wolfram] Univ Vienna, A-1010 Vienna, Austria. RP Bastow, R (reprint author), Univ Warwick, Coventry CV4 7AL, W Midlands, England. RI Meyers, Blake/B-6535-2012; Mayer, Klaus/M-7941-2015; Carrington, James/A-4656-2012; Weckwerth, Wolfram/G-5811-2010; Gutierrez, Rodrigo/C-2611-2008; Millar, Andrew/G-2423-2012; may, sean/B-6838-2012; Kudla, Jorg/C-2174-2012; Millar, A. Harvey/A-5452-2008; Ecker, Joseph/B-9144-2008; Braun, Pascal/B-9669-2013; Matsui, Minami/A-5235-2016; Mockler, Todd/L-2609-2013 OI Schroeder, Julian/0000-0002-3283-5972; Kersey, Paul/0000-0002-7054-800X; Birney, Ewan/0000-0001-8314-8497; Rawlings, Christopher/0000-0003-3702-1633; Meyers, Blake/0000-0003-3436-6097; Mayer, Klaus/0000-0001-6484-1077; Carrington, James/0000-0003-3572-129X; Millar, Andrew/0000-0003-1756-3654; may, sean/0000-0001-5282-3250; Kudla, Jorg/0000-0002-8238-767X; Millar, A. Harvey/0000-0001-9679-1473; Ecker, Joseph/0000-0001-5799-5895; Braun, Pascal/0000-0003-2012-6746; Matsui, Minami/0000-0001-5162-2668; Mockler, Todd/0000-0002-0462-5775 NR 2 TC 15 Z9 15 U1 2 U2 18 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD AUG PY 2010 VL 22 IS 8 BP 2530 EP 2536 DI 10.1105/tpc.110.078519 PG 7 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 655SU UT WOS:000282270600004 ER PT J AU Lin, JY Stupar, RM Hans, C Hyten, DL Jackson, SA AF Lin, Jer-Young Stupar, Robert M. Hans, Christian Hyten, David L. Jackson, Scott A. TI Structural and Functional Divergence of a 1-Mb Duplicated Region in the Soybean (Glycine max) Genome and Comparison to an Orthologous Region from Phaseolus vulgaris SO PLANT CELL LA English DT Article ID BACTERIAL ARTIFICIAL CHROMOSOMES; MUTATION-RATE VARIATION; GENE-EXPRESSION; DROSOPHILA-MELANOGASTER; TRANSPOSABLE ELEMENTS; EVOLUTIONARY EVENTS; MESSENGER-RNA; MAIZE GENOME; L. MERR.; SEQUENCE AB Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two similar to 1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent similar to 13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in Gm8) and a nearly twofold increase in the deletion of long terminal repeat (LTR) retrotransposons via solo LTR formation. Functional analyses of Gm15 and Gm8 revealed decreases in gene expression and synonymous substitution rates for Gm15, for instance, a 38% increase in transcript levels from Gm8 relative to Gm15. Transcriptional divergence of homoeologs was found based on expression patterns among seven tissues and developmental stages. Our results indicate asymmetric evolution between homoeologous regions of soybean as evidenced by structural changes and expression variances of homoeologous genes. C1 [Lin, Jer-Young; Hans, Christian; Jackson, Scott A.] Purdue Univ, W Lafayette, IN 47907 USA. [Stupar, Robert M.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Hyten, David L.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Jackson, SA (reprint author), Purdue Univ, W Lafayette, IN 47907 USA. EM sjackson@purdue.edu OI Hyten, David/0000-0001-6324-9389; Stupar, Robert/0000-0002-8836-2924 FU National Science Foundation [DBI 0501877, IOS 0822258] FX We thank Dinesha Walek and William Haun for providing assistance in generating the homoeologous transcription data. We thank the National Science Foundation (DBI 0501877 and IOS 0822258) for funding to S.A.J. to support this work. NR 97 TC 34 Z9 34 U1 0 U2 15 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD AUG PY 2010 VL 22 IS 8 BP 2545 EP 2561 DI 10.1105/tpc.110.074229 PG 17 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 655SU UT WOS:000282270600006 PM 20729383 ER PT J AU Harman, GE Obregon, MA Samuels, GJ Lorito, M AF Harman, Gary E. Obregon, Miguel A. Samuels, Gary J. Lorito, Matteo TI Changing Models for Commercialization and Implementation of Biocontrol in the Developing and the Developed World SO PLANT DISEASE LA English DT Article C1 [Harman, Gary E.] Cornell Univ, Geneva, NY USA. [Obregon, Miguel A.] Asesoramiento Fitosanitario Lab Doctor Obregon, La Aurora De Herdia, Costa Rica. [Samuels, Gary J.] USDA, Agr Res Serv, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. [Lorito, Matteo] Univ Naples Federico II, Naples, Italy. RP Harman, GE (reprint author), Cornell Univ, Hort Sci, 630 W North St, Geneva, NY 14456 USA. EM geh3@cornell.edu OI LORITO, Matteo/0000-0002-0529-3709 FU Advanced Biological Marketing, Van Wert, OH from USDA Small Business Innovation Research [2008-00169, 2009-01133]; Hatch formula funds; Cornell University Center for Advanced Technology (NYSTAR) FX This article arose as a consequence of the X Trichoderma and Gliocladium Conference in San Jose, Costa Rica, on 21-23 May 2008. Additional information and perspectives were obtained at the "Expert Consultation on Biopesticides and BioFertilizers in Sustainable Agriculture," which was organized by the Association of Asian Pacific Agricultural Research Institutions (AAPARI) in Taichung, Chinese Taipei, by the senior author. The research of the senior author was supported in part by Advanced Biological Marketing, Van Wert, OH, as a subcontract from USDA Small Business Innovation Research grants 2008-00169 and 2009-01133, from Hatch formula funds, and by funding from the Cornell University Center for Advanced Technology (NYSTAR). It should be noted that the first, second, and fourth authors have financial interests in the work described in this paper. NR 68 TC 26 Z9 30 U1 1 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 928 EP 939 DI 10.1094/PDIS-94-8-0928 PG 12 WC Plant Sciences SC Plant Sciences GA V40YC UT WOS:000209512900001 ER PT J AU Nakhla, MK Owens, KJ Li, WB Wei, G Skantar, AM Levy, L AF Nakhla, Mark K. Owens, Kristina J. Li, Wenbin Wei, Gang Skantar, Andrea M. Levy, Laurene TI Multiplex Real-Time PCR Assays for the Identification of the Potato Cyst and Tobacco Cyst Nematodes SO PLANT DISEASE LA English DT Article ID GLOBODERA-TABACUM-TABACUM; T-SOLANACEARUM NEMATA; DNA-SEQUENCES; PALLIDA; POPULATIONS; PLANTS; HETERODERINAE; VIRGINIAE; DIFFERENTIATION; ROSTOCHIENSIS AB TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set specific for G. pallida (pale potato cyst nematode) multiplexed with another primer-probe set specific for G. rostochiensis (golden potato cyst nematode). A second tube consisted of the G. pallida-specific primer-probe set multiplexed with a primer-probe set specific for G. tabacum (the morphologically similar tobacco cyst nematode). This internal transcribed spacer rDNA-based system was specific for the Globodera spp. of interest and successfully identified several populations of PCN. This rapid, sensitive, and specific quantitative PCR assay presents a useful tool for PCN regulatory response and management programs. C1 [Nakhla, Mark K.; Owens, Kristina J.; Li, Wenbin; Wei, Gang; Levy, Laurene] APHIS, Natl Plant Germplasm & Biotechnol Lab, USDA, PPQ CPHST, Beltsville, MD 20705 USA. [Skantar, Andrea M.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Nakhla, MK (reprint author), APHIS, Natl Plant Germplasm & Biotechnol Lab, USDA, PPQ CPHST, Beltsville, MD 20705 USA. EM Mark.Nakhla@aphis.usda.gov NR 26 TC 16 Z9 18 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 959 EP 965 DI 10.1094/PDIS-94-8-0959 PG 7 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900004 ER PT J AU Durrin, JS Nikolaeva, OV Strausbaugh, CA Karasev, AV AF Durrin, Jenny S. Nikolaeva, Olga V. Strausbaugh, Carl A. Karasev, Alexander V. TI Immunodetection of Two Curtoviruses Infecting Sugar Beet SO PLANT DISEASE LA English DT Article ID CURLY-TOP-VIRUS; LINKED IMMUNOSORBENT ASSAY; WESTERN UNITED-STATES; COAT PROTEIN; NUCLEOTIDE-SEQUENCE; POLYCLONAL ANTISERA; ESCHERICHIA-COLI; GEMINIVIRUSES; RESISTANCE; GENE AB Beet leafhopper-transmitted curly top virus is a serious problem in many different crops in the semiarid western United States, including sugar beet, tomatoes, and beans. Curly top is caused by a genetically diverse complex of phloem-limited curtoviruses. Due to the phloem restriction of curtoviruses and the lack of a convenient laboratory host vector system for curly top virus propagation and purification, no commercial immunodetection tests are available for curtoviruses. Routine diagnostics for curly top rely either on visual symptoms or on polymerase chain reaction (PCR) tests. Lack of an enzyme-linked immunosorbent assay (ELISA) system is one of the factors hampering development and screening of the curly top resistant germplasm in, for instance, sugar beet and bean breeding programs. To fill in this gap, we developed an ELISA-based detection system for curtoviruses which utilizes virus-specific antibodies generated against bacterially expressed capsid protein (CP) of Beet mild curly top virus. Bacterially expressed CP was affinity purified and used as an antigen for antibody production in two animal species. Specificity of the resulting antisera was tested in Western blots and various triple-antibody sandwich (TAS)-ELISA formats with sugar beet, bean, and Nicotiana benthamiana leaf tissue. We demonstrate reliable detection of two curtoviruses in different crops in TAS-ELISA format, suitable for large-scale screening of germplasm in breeding programs. C1 [Durrin, Jenny S.; Nikolaeva, Olga V.; Karasev, Alexander V.] Univ Idaho, Dept PSES, Moscow, ID 83844 USA. [Strausbaugh, Carl A.] USDA ARS, Kimberly, ID 83341 USA. RP Karasev, AV (reprint author), Univ Idaho, Dept PSES, Moscow, ID 83844 USA. EM akarasev@uidaho.edu OI Strausbaugh, Carl/0000-0001-6773-1375 FU Idaho Bean Commission; Idaho Sugar Beet Industry; NIH [P20 RR016454] FX We are grateful to W. Wintermantel (USDA-ARS, Salinas, CA) for providing infected material with individual strains of curly top virus, to R. Gilbertson (University of California, Davis) for help with curtovirus detection by PCR, and to Teresa Meacham for assistance with PCR tests. This work was funded in part through grants from the Idaho Bean Commission and from the Idaho Sugar Beet Industry. J. S. Durrin was a recipient of an Idaho INBRE undergraduate fellowship funded through the NIH Grant # P20 RR016454. NR 28 TC 5 Z9 5 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 972 EP 976 DI 10.1094/PDIS-94-8-0972 PG 5 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900006 ER PT J AU Weiland, JE Nelson, AH Hudler, GW AF Weiland, Jerry E. Nelson, Angela H. Hudler, George W. TI Aggressiveness of Phytophthora cactorum, P. citricola I, and P. plurivora from European Beech SO PLANT DISEASE LA English DT Article ID SP-NOV.; FAGUS-SYLVATICA; FOREST TREES; SUSCEPTIBILITY; CITROPHTHORA; DIVERSITY; RAMORUM; CROPS; ROOT; ROT AB Phytophthora cactorum, P citricola I, and P plurivora cause bleeding cankers on mature European beech (Fagus sylvatica) trees in the northeastern United States. Inoculation experiments were conducted to compare the aggressiveness of the three Phytophthora spp. on stems, leaf disks, and roots of European beech and common lilac (Syringa vulgaris) seedlings. Isolates were obtained from bleeding cankers on European beech from five cities in New York (Albany, Ithaca, Oyster Bay, Plainview, and Rochester) and from a bleeding canker on sugar maple in Ithaca, NY. Stems were inoculated with colonized agar plugs, leaf disks with a zoospore suspension, and roots via infested soil at three inoculum levels. All organs of inoculated beech and lilac developed disease except for lilac roots inoculated with zoospores of P cactorum. Disease incidence, severity, and plant survival were dependent on isolate and were also influenced by the tissue inoculated and host. Isolates of P cactorum were the least aggressive and caused less necrosis than isolates of P citricola I and P plurivora. Results emphasize the utility of stem and root inoculation for evaluation of this canker disease and underscore critical differences in species aggressiveness. C1 [Weiland, Jerry E.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. [Weiland, Jerry E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Nelson, Angela H.; Hudler, George W.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14850 USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. EM Jerry.Weiland@ars.usda.gov FU Agri-Chem Inc.; Rainbow Tree Care Scientific Advancements Inc.; Cornell University [NYC-1536413] FX Research reported herein was supported in part by grants from Agri-Chem Inc., Rainbow Tree Care Scientific Advancements Inc., and the Cornell University Agricultural Experiment Station Federal Formula Funds, Project No. NYC-1536413 received from the Cooperative State Research, Education and Extension Service, United States Department of Agriculture. NR 25 TC 5 Z9 6 U1 1 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 1009 EP 1014 DI 10.1094/PDIS-94-8-1009 PG 6 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900011 ER PT J AU Nagle, AM Long, RP Madden, LV Bonello, P AF Nagle, Annemarie M. Long, Robert P. Madden, Laurence V. Bonello, Pierluigi TI Association of Phytophthora cinnamomi with White Oak Decline in Southern Ohio SO PLANT DISEASE LA English DT Article ID CLIMATE-CHANGE; QUERCUS-ROBUR; SITE FACTORS; FORESTS; DISEASE; SEEDLINGS; INVOLVEMENT; STANDS; RANGE; SOILS AB A decline syndrome and widespread mortality of mature white oak tree (Quercus alba) associated with wet and low-lying areas has been recently observed in southern Ohio forests. Previous studies have isolated Phytophthora cinnamomi from white oak rhizospheres. In 2008 and 2009, P cinnamomi population densities in two healthy and two declining white oak stands at Scioto Trail State Forest were quantified and potential roles of three environmental drivers of Phytophthora spp.-induced decline were assessed: soil texture, soil moisture, and topography. Significantly higher P cinnamomi propagule densities were found in declining stands in both years but propagule densities were not associated with soil moisture content. Trends in population densities were not correlated with soil moisture or topographic position within field sites. There was a positive, exponential relationship between overall P cinnamomi population levels and soil moisture on a seasonal scale in 2008 but not 2009. Sites with greater soil clay content were associated with greater decline. Effects of P cinnamomi inoculum and periodic flooding on root health of 1-year-old potted white oak trees grown in native soil mixes in the greenhouse were examined. Root systems of potted oak were significantly damaged by soil inoculation with P cinnamomi, especially under flooding conditions. Results of these studies support the hypothesis that P cinnamomi is a contributing agent to white oak decline in southern Ohio. C1 [Nagle, Annemarie M.; Bonello, Pierluigi] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Madden, Laurence V.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Long, Robert P.] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. RP Bonello, P (reprint author), Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. EM bonello.2@osu.edu RI Bonello, Pierluigi/E-2776-2011 FU Ohio Agricultural Research and Development Center; Department of Plant Pathology, The Ohio State University FX Funding for this research was provided by an Ohio Agricultural Research and Development Center grant to A. M. Nagle and state funds appropriated to the Department of Plant Pathology, The Ohio State University. We thank Y. Balci for assistance with experimental design and procedures; T. Fox for extensive assistance with fieldwork and soil texture analyses; and C. Rico, S. Quinn, C. Baker, A. Hoffstetter, R. Snyder, and W. Hilinski for their assistance in field work and laboratory assays. NR 35 TC 12 Z9 13 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 1026 EP 1034 DI 10.1094/PDIS-94-8-1026 PG 9 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900014 ER PT J AU Li, SX Hartman, GL Boykin, DL AF Li, Shuxian Hartman, Glen L. Boykin, Deborah L. TI Aggressiveness of Phomopsis longicolla and Other Phomopsis spp. on Soybean SO PLANT DISEASE LA English DT Article ID SP SEED INFECTION; MOLECULAR-IDENTIFICATION; DIAPORTHE-PHASEOLORUM; 1ST REPORT; DECAY; RESISTANCE; QUALITY; FUNGI; CULTIVAR; PODS AB Phomopsis seed decay of soybean is a major cause of poor-quality soybean seed. The disease is caused primarily by the fungal pathogen Phomopsis longicolla. Aggressiveness of isolates of P. longicolla from soybean and other Phomopsis spp. from other hosts were compared by inoculating 2-week-old soybean plants of cv. Williams 82. There were significant (P <= 0.0001) differences among isolates based on stem length and stem lesion length. The P longicolla soybean isolate PL16, from Mississippi, caused the shortest stem length while the non-soybean isolate P9, from Illinois, caused the greatest stem lesion length. The type isolate of P longicolla, PL31 (Fau 600), was one of the 3 most aggressive isolates among all 48 isolates tested. The velvetleaf isolate P9 was the most aggressive among 13 isolates from non-soybean hosts. This study provided the first evaluation of aggressiveness of P. longicolla isolates from different geographic origins and the first demonstration that Phomopsis spp. isolated from cantaloupe, eggplant, and watermelon infected soybean. Knowledge about the variability of the pathogen is important for selecting isolates for breeding soybean lines with broad-based resistance to Phomopsis seed decay. C1 [Li, Shuxian] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. [Boykin, Deborah L.] USDA ARS, Midsouth Area Off, Stoneville, MS 38776 USA. RP Li, SX (reprint author), USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM shuxian.li@ars.usda.gov FU United Soybean Board; Illinois Soybean Association; North Central Soybean Research Program; United States Department of Agriculture-Agricultural Research Service; Cooperative State Research, Education, and Extension Service FX We thank C. R. Grau, A. Rossman, and J. Rupe for providing fungal cultures or diseased plant samples; and J. King and C. You for assisting with the pathogenicity tests. This research was partially supported by the United Soybean Board, Illinois Soybean Association, North Central Soybean Research Program, and United States Department of Agriculture-Agricultural Research Service and Cooperative State Research, Education, and Extension Service. NR 30 TC 25 Z9 29 U1 0 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 1035 EP 1040 DI 10.1094/PDIS-94-8-1035 PG 6 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900015 ER PT J AU Deahl, KL Perez, F Baker, CJ Jones, RW Cooke, L McGrath, M AF Deahl, K. L. Perez, F. Baker, C. J. Jones, R. W. Cooke, L. McGrath, M. TI Natural Occurrence of Phytophthora infestans Causing Late Blight on Woody Nightshade (Solanum dulcamara) in New York SO PLANT DISEASE LA English DT News Item C1 [Deahl, K. L.; Perez, F.; Baker, C. J.; Jones, R. W.] USDA ARS, Beltsville, MD 20705 USA. [McGrath, M.] Cornell Univ, Riverhead, NY 11901 USA. [Cooke, L.] ASFBI, Belfast BT9 5PX, Antrim, North Ireland. RP Deahl, KL (reprint author), USDA ARS, Beltsville, MD 20705 USA. NR 4 TC 2 Z9 3 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2010 VL 94 IS 8 BP 1063 EP 1063 DI 10.1094/PDIS-94-8-1063B PG 1 WC Plant Sciences SC Plant Sciences GA 627RI UT WOS:000280058900023 ER PT J AU Ren, H Ma, GH Zhang, QM Guo, QF Wang, J Wang, ZF AF Ren, Hai Ma, Guohua Zhang, Qianmei Guo, Qinfeng Wang, Jun Wang, Zhengfeng TI Moss is a key nurse plant for reintroduction of the endangered herb, Primulina tabacum Hance SO PLANT ECOLOGY LA English DT Article DE Conservation; Moss; Nurse plant; Primulina tabacum; Reintroduction; Survival rate ID CONSERVATION; RESTORATION; ECOLOGY AB The rare and endangered plant Primulina tabacum is a calciphilous perennial herb found only at the entrances of a small number of karst cave drainages in southern China. In a conservation effort, we identified potentially suitable habitats and reintroduced P. tabacum plantlets (propagated in vitro) to one historical and two new cave entrances. The transplanted seedlings survived (10%) at only one new location where a moss, Gymnostomiella longinervis Broth, existed. Our field observations indicate that it is probably impossible for this rare plant to naturally recolonize the places where it went extinct because the habitats have changed. Transplanted P. tabacum grew slower than wild P. tabacum. The transplanted P. tabacum performed especially well under the cover of the nursing moss. Positive interactions between species, i.e., nurse plant effects, are important for reintroduction of success. Although light and soil conditions also appeared to be critical for transplantation success, the presence of moss should be considered as a useful and convenient indicator of suitable habitat for P. tabacum. This study case suggests that the use of new propagation methods and nurse plants can facilitate the reintroduction of rare and endangered herbs. C1 [Ren, Hai; Ma, Guohua; Zhang, Qianmei; Wang, Jun; Wang, Zhengfeng] Chinese Acad Sci, S China Bot Garden, Dept Ecol, Guangzhou 510650, Guangdong, Peoples R China. [Guo, Qinfeng] USDA FS Eastern Forest Threat Ctr, Asheville, NC 28804 USA. RP Ren, H (reprint author), Chinese Acad Sci, S China Bot Garden, Dept Ecol, Guangzhou 510650, Guangdong, Peoples R China. EM renhai@scib.ac.cn FU National Basic Research Program of China [2008BAJ10B03, 2007CB411600]; Guangdong Sci-Tech Planning Project [07118249, 2008A060207017, 2007A060306011] FX This research was supported by the National Basic Research Program of China (2008BAJ10B03, 2007CB411600) and the Guangdong Sci-Tech Planning Project (07118249, 2008A060207017, 2007A060306011). We thank Mr. Xiao Lei for monitoring, Mr. Guowei Chu for nutrient element analysis, Prof. Li Zhang for moss species identification, Prof. Chonghui Liao for the insect species identification, and Dr. Shijin Li, Dr. Wenjun Duan, Mr. Xiangxu Huang, and Miss Changxin He for fieldwork assistance. We also thank the anonymous reviewers for their valuable comments on an earlier version of the manuscript. Thanks also to Prof. Bruce Jaffee for polishing the English. NR 39 TC 14 Z9 20 U1 10 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD AUG PY 2010 VL 209 IS 2 SI SI BP 313 EP 320 DI 10.1007/s11258-010-9754-5 PG 8 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 620DP UT WOS:000279479500012 ER PT J AU Motilal, LA Zhang, DP Umaharan, P Mischke, S Mooleedhar, V Meinhardt, LW AF Motilal, Lambert A. Zhang, Dapeng Umaharan, Pathmanathan Mischke, Sue Mooleedhar, Vishnarayan Meinhardt, Lyndel W. TI The relic Criollo cacao in Belize - genetic diversity and relationship with Trinitario and other cacao clones held in the International Cocoa Genebank, Trinidad SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE cacao; Criollo; genebank; genetic contribution; genetic uniformity; microsatellite; population structure; synonymous groups; Trinitario ID THEOBROMA-CACAO; POPULATION-STRUCTURE; NATURAL-POPULATIONS; PHYLOGENETIC TREES; COMPUTER-PROGRAM; L. GERMPLASM; IDENTIFICATION; INFERENCE; ACCURACY; ECUADOR AB Cacao (Theobroma cacao L.) was domesticated in Mesoamerica and is native to the South American rainforest. Belizean Criollo is a group of relic landraces that are thought to be similar to those used by the Olmecs and Mayans during early domestication. Knowledge of genetic diversity is essential for efficient conservation and use of these relic landraces. Using 30 micro-satellite markers, we characterized genetic diversity in 77 Belize Criollo accessions collected from the Maya Mountains in Belize, and assessed their relationship with 62 cacao accessions including 25 Trinitario accessions. Genetic diversity and heterozygosity were low in Belizean Criollo germplasm. Eleven distinctive genotypes were identified among the Belizean germplasm. Results of ordination and cluster analysis supported their putative ancestral contribution to the Trinitario cacao. However, results of Bayesian assignment and parentage analysis both suggested that the contribution of Criollo cacao to the Imperial College Selections Trinitario is small. Our preferred hypothesis for the genesis of Trinitario cacao is that a limited population of Criollo X Forastero hybrids emanated from the introduced Forastero population of Trinidad. The present study provides new insights into the origin of the Trinitario cacao, which will be useful in the ex situ and in situ conservation of cacao landraces from Mesoamerica. C1 [Motilal, Lambert A.; Mooleedhar, Vishnarayan] Univ W Indies, Cocoa Res Unit, St Augustine, Trinidad, Trinid & Tobago. [Zhang, Dapeng; Mischke, Sue; Meinhardt, Lyndel W.] USDA ARS, Beltsville Agr Res Ctr, PSI, SPCL 10300,BARC W, Beltsville, MD 20705 USA. [Umaharan, Pathmanathan] Univ W Indies, Fac Sci & Agr, Sch Plant Sci, St Augustine, Trinidad, Trinid & Tobago. [Mooleedhar, Vishnarayan] Univ Trinidad & Tobago, Valsayn, Trinidad, Trinid & Tobago. RP Motilal, LA (reprint author), Univ W Indies, Cocoa Res Unit, St Augustine, Trinidad, Trinid & Tobago. EM lamotilal@yahoo.com FU National Geographic Society FX The financial support of the Biscuit Cake Chocolate Confectionery Alliance (now Cocoa Research Association Ltd) of the United Kingdom towards the germplasm mission to Belize is gratefully acknowledged. The Maya Mountain Archaeological Project was partially funded by the National Geographic Society. Kevin Moro, Perfecto Nal and Isidro Bol of the Belizean Defence Force as well as Irideo Bolen and Hugh O'Brien of the Ministry of Agriculture of Belize among others were invaluable to the expedition. Winston Maharaj of the Cocoa Research Unit is also thanked for his role in the expedition. We also thank Dr David Butler, then head of Cocoa Research Unit, for his permission to use the Belizean Criollo material and support for this paper. Two anonymous reviewers are thanked for checking the manuscript. NR 46 TC 14 Z9 14 U1 0 U2 8 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD AUG PY 2010 VL 8 IS 2 BP 106 EP 115 DI 10.1017/S1479262109990232 PG 10 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA 635WA UT WOS:000280686800002 ER PT J AU Du, JC Tian, ZX Hans, CS Laten, HM Cannon, SB Jackson, SA Shoemaker, RC Ma, JX AF Du, Jianchang Tian, Zhixi Hans, Christian S. Laten, Howard M. Cannon, Steven B. Jackson, Scott A. Shoemaker, Randy C. Ma, Jianxin TI Evolutionary conservation, diversity and specificity of LTR-retrotransposons in flowering plants: insights from genome-wide analysis and multi-specific comparison SO PLANT JOURNAL LA English DT Article DE centromere retrotransposons; plant retroviruses; LTR-retrotransposons; genome evolution; comparative genomics ID MAIZE GENOME; GLYCINE-MAX; INFECTIOUS RETROVIRUS; TRANSPOSABLE ELEMENTS; CENTROMERIC REGION; CEREAL CHROMOSOMES; SATELLITE REPEAT; GENE DUPLICATION; RICE CENTROMERE; DNA ELEMENT AB P>The availability of complete or nearly complete genome sequences from several plant species permits detailed discovery and cross-species comparison of transposable elements (TEs) at the whole genome level. We initially investigated 510 long terminal repeat-retrotransposon (LTR-RT) families comprising 32 370 elements in soybean (Glycine max (L.) Merr.). Approximately 87% of these elements were located in recombination-suppressed pericentromeric regions, where the ratio (1.26) of solo LTRs to intact elements (S/I) is significantly lower than that of chromosome arms (1.62). Further analysis revealed a significant positive correlation between S/I and LTR sizes, indicating that larger LTRs facilitate solo LTR formation. Phylogenetic analysis revealed seven Copia and five Gypsy evolutionary lineages that were present before the divergence of eudicot and monocot species, but the scales and timeframes within which they proliferated vary dramatically across families, lineages and species, and notably, a Copia lineage has been lost in soybean. Analysis of the physical association of LTR-RTs with centromere satellite repeats identified two putative centromere retrotransposon (CR) families of soybean, which were grouped into the CR (e.g. CRR and CRM) lineage found in grasses, indicating that the 'functional specification' of CR pre-dates the bifurcation of eudicots and monocots. However, a number of families of the CR lineage are not concentrated in centromeres, suggesting that their CR roles may now be defunct. Our data also suggest that the envelope-like genes in the putative Copia retrovirus-like family are probably derived from the Gypsy retrovirus-like lineage, and thus we propose the hypothesis of a single ancient origin of envelope-like genes in flowering plants. C1 [Cannon, Steven B.; Shoemaker, Randy C.] ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Du, Jianchang; Tian, Zhixi; Hans, Christian S.; Jackson, Scott A.; Ma, Jianxin] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Laten, Howard M.] Loyola Univ, Dept Biol, Chicago, IL 60660 USA. RP Shoemaker, RC (reprint author), ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM randy.shoemaker@ars.usda.gov FU USDA-ARS; Purdue University; National Science Foundation [DBI-0822258] FX We thank Dr Vini Pereira for providing an Arabidopsis transposable elements dataset. This study was supported by USDA-ARS Specific Cooperative Agreement to JM, Purdue University faculty startup funds to JM, and National Science Foundation Plant Genome Research Program (DBI-0822258) to SAJ, RCS, and JM. NR 76 TC 60 Z9 66 U1 1 U2 21 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0960-7412 J9 PLANT J JI Plant J. PD AUG PY 2010 VL 63 IS 4 BP 584 EP 598 DI 10.1111/j.1365-313X.2010.04263.x PG 15 WC Plant Sciences SC Plant Sciences GA 639VE UT WOS:000281002100005 PM 20525006 ER PT J AU Glynn, NC Dixon, LJ Castlebury, LA Szabo, LJ Comstock, JC AF Glynn, N. C. Dixon, L. J. Castlebury, L. A. Szabo, L. J. Comstock, J. C. TI PCR assays for the sugarcane rust pathogens Puccinia kuehnii and P. melanocephala and detection of a SNP associated with geographical distribution in P. kuehnii SO PLANT PATHOLOGY LA English DT Article DE brown rust of sugarcane; locked nucleic acids; orange rust of sugarcane; PIRA-PCR; real-time PCR; Saccharum spp. ID ORANGE RUST; 1ST REPORT; CAUSAL AGENT; STRIIFORMIS; TRITICI; SPREAD AB Puccinia kuehnii and P. melanocephala cause orange and brown rust of sugarcane, respectively. Puccinia kuehnii has been confirmed in Asia, Australia and recently, the Caribbean basin, whereas P. melanocephala is distributed among the majority of sugarcane growing regions. Differentiating these two economically significant pathogens visually is problematic and limited to material exhibiting mature disease symptoms or spores. Partial ITS1, ITS2 and complete 5.8S sequences were generated from P. kuehnii and P. melanocephala isolates from around the world. PCR primers and dual labelled hydrolysis probes were designed for each pathogen for use in real-time PCR and optimized using locked nucleic acids (LNA). The primers amplified DNA from their target pathogens and not from other species of Puccinia or fungal species isolated from sugarcane leaves. Optimized real-time PCR conditions allowed the detection of 0 19 pg of P. kuehnii or P. melanocephala genomic DNA and differentiated the pathogens on sugarcane leaves prior to observing typical symptoms in the field. Primer-introduced restriction analysis-PCR (PIRA-PCR) was used to detect a single nucleotide polymorphism (Pk ITS1 183A>G) in ITS1 of P. kuehnii. Allele 183A was observed in all samples, whereas 183G was detected in 52% of samples from Asia and Australia yet absent from all Caribbean basin samples. Long distance spore dispersal, dispersal through an intermediate location or improper movement of contaminated material could explain the introduction of P. kuehnii to the Western hemisphere. However, the current proliferation of the pathogen in the Americas is limited to isolates which contain only the 183A allele. C1 [Glynn, N. C.; Comstock, J. C.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Dixon, L. J.; Castlebury, L. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Szabo, L. J.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Glynn, NC (reprint author), USDA ARS, Sugarcane Field Stn, 12990 US Hwy 441N, Canal Point, FL 33438 USA. EM neil.glynn@ars.usda.gov NR 24 TC 18 Z9 23 U1 0 U2 12 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0032-0862 J9 PLANT PATHOL JI Plant Pathol. PD AUG PY 2010 VL 59 IS 4 BP 703 EP 711 DI 10.1111/j.1365-3059.2010.02299.x PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 619TB UT WOS:000279452100012 ER PT J AU Attanayake, RN Glawe, DA McPhee, KE Dugan, FM Chen, W AF Attanayake, R. N. Glawe, D. A. McPhee, K. E. Dugan, F. M. Chen, W. TI Erysiphe trifolii - a newly recognized powdery mildew pathogen of pea SO PLANT PATHOLOGY LA English DT Article DE chasmothecial appendages; ITS sequences; Pisum sativum; powdery mildew; single nucleotide polymorphism; taxonomy ID MOLECULAR-IDENTIFICATION; RESISTANCE; PHYLOGENY; SEQUENCES; YIELD; GENE; PISI AB Diversity of powdery mildew pathogens infecting pea (Pisum sativum) in the US Pacific Northwest was investigated using both molecular and morphological techniques. Phylogenetic analyses based on rDNA ITS sequences, in combination with assessment of morphological characters, defined two groups of powdery mildews infecting pea. Group I (five field samples and three glasshouse samples) had ITS sequences 99% similar to those of Erysiphe pisi in GenBank and exhibited simple, mycelioid type of chasmothecial appendages typical of E. pisi. Erysiphe pisi is normally considered as the powdery mildew pathogen of pea. Group II (four glasshouse samples and two field samples) had ITS sequences 99% similar to those of E. trifolii and produced chasmothecia with dichotomously branched appendages similar to those of E. trifolii. There are fourteen nucleotide differences in the ITS region between the two groups. The correlation of rDNA ITS sequences with teleomorphic features for each of the two groups confirms their identity. Repeated samplings and artificial inoculations indicate that both E. pisi and E. trifolii infect pea in the US Pacific Northwest. Erysiphe trifolii is not previously known as a pathogen of pea. The existence of two distinct powdery mildew species infecting pea in both glasshouse and field environments may interfere with the powdery mildew-resistance breeding programmes, and possibly explains putative instances of breakdown of resistance in previously resistant pea breeding lines. C1 [Attanayake, R. N.; Glawe, D. A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Glawe, D. A.] Univ Washington, Coll Forest Resources, Seattle, WA 98195 USA. [McPhee, K. E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Dugan, F. M.; Chen, W.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Attanayake, RN (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM w-chen@wsu.edu FU USDA-CSREES FX The authors want to thank Professor Uwe Braun, Martin-Luther-Universitat, Institut fur Biologie, Bereich Geobotanik, Herbarium, Neuwerk 21, D-06099 Halle/S. Germany for advice concerning the taxonomic status of Erysiphe trifolii, Dr Eric H. Roalson, Department of Biology, Washington State University, Pullman, WAfor helping in phylogenetic analysis, and Shari Lupien, Plant Introduction, USDA ARS, Pullman for maintaining Melilotus albus plants infected with powdery mildew. The research was funded in part by the USDA-CSREES Cool Season Food Legume Research Program. NR 36 TC 15 Z9 17 U1 1 U2 4 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0032-0862 J9 PLANT PATHOL JI Plant Pathol. PD AUG PY 2010 VL 59 IS 4 BP 712 EP 720 DI 10.1111/j.1365-3059.2010.02306.x PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 619TB UT WOS:000279452100013 ER PT J AU Freeman, JL Tamaoki, M Stushnoff, C Quinn, CF Cappa, JJ Devonshire, J Fakra, SC Marcus, MA McGrath, SP Van Hoewyk, D Pilon-Smits, EAH AF Freeman, John L. Tamaoki, Masanori Stushnoff, Cecil Quinn, Colin F. Cappa, Jennifer J. Devonshire, Jean Fakra, Sirine C. Marcus, Matthew A. McGrath, Steve P. Van Hoewyk, Doug Pilon-Smits, Elizabeth A. H. TI Molecular Mechanisms of Selenium Tolerance and Hyperaccumulation in Stanleya pinnata SO PLANT PHYSIOLOGY LA English DT Article ID SYSTEMIC ACQUIRED-RESISTANCE; PRAIRIE DOG HERBIVORY; VITAMIN-C CONTENTS; ARABIDOPSIS-THALIANA; SELENOCYSTEINE METHYLTRANSFERASE; CELLULAR COMPARTMENTATION; ASTRAGALUS-BISULCATUS; ACCUMULATING PLANTS; INDICATOR PLANTS; INDIAN MUSTARD AB The molecular mechanisms responsible for selenium (Se) tolerance and hyperaccumulation were studied in the Se hyper-accumulator Stanleya pinnata (Brassicaceae) by comparing it with the related secondary Se accumulator Stanleya albescens using a combination of physiological, structural, genomic, and biochemical approaches. S. pinnata accumulated 3.6-fold more Se and was tolerant to 20 mu M selenate, while S. albescens suffered reduced growth, chlorosis and necrosis, impaired photosynthesis, and high levels of reactive oxygen species. Levels of ascorbic acid, glutathione, total sulfur, and nonprotein thiols were higher in S. pinnata, suggesting that Se tolerance may in part be due to increased antioxidants and up-regulated sulfur assimilation. S. pinnata had higher selenocysteine methyltransferase protein levels and, judged from liquid chromatography-mass spectrometry, mainly accumulated the free amino acid methylselenocysteine, while S. albescens accumulated mainly the free amino acid selenocystathionine. S. albescens leaf x-ray absorption near-edge structure scans mainly detected a carbon-Se-carbon compound (presumably selenocystathionine) in addition to some selenocysteine and selenate. Thus, S. albescens may accumulate more toxic forms of Se in its leaves than S. pinnata. The species also showed different leaf Se sequestration patterns: while S. albescens showed a diffuse pattern, S. pinnata sequestered Se in localized epidermal cell clusters along leaf margins and tips, concentrated inside of epidermal cells. Transcript analyses of S. pinnata showed a constitutively higher expression of genes involved in sulfur assimilation, antioxidant activities, defense, and response to (methyl) jasmonic acid, salicylic acid, or ethylene. The levels of some of these hormones were constitutively elevated in S. pinnata compared with S. albescens, and leaf Se accumulation was slightly enhanced in both species when these hormones were supplied. Thus, defense-related phytohormones may play an important signaling role in the Se hyperaccumulation of S. pinnata, perhaps by constitutively up-regulating sulfur/Se assimilation followed by methylation of selenocysteine and the targeted sequestration of methylselenocysteine. C1 [Freeman, John L.; Quinn, Colin F.; Cappa, Jennifer J.; Van Hoewyk, Doug; Pilon-Smits, Elizabeth A. H.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Stushnoff, Cecil] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. [Freeman, John L.] ARS, USDA, Water Management Res Div, Parlier, CA 93648 USA. [Freeman, John L.] Calif State Univ Fresno, Ctr Irrigat Technol, Fresno, CA 93740 USA. [Tamaoki, Masanori] Natl Inst Environm Studies, Div Environm Biol, Tsukuba, Ibaraki 3058506, Japan. [Devonshire, Jean; McGrath, Steve P.] Rothamsted Res, Harpenden AL5 2JQ, Herts, England. [Fakra, Sirine C.; Marcus, Matthew A.] Univ Calif Berkeley, Lawrence Berkeley Lab, Adv Light Source, Berkeley, CA 94720 USA. [Van Hoewyk, Doug] Coastal Carolina Univ, Dept Biol, Conway, SC 29526 USA. RP Pilon-Smits, EAH (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM epsmits@lamar.colostate.edu RI McGrath, Steve/B-5127-2008; OI McGrath, Steve/0000-0003-0952-8947; Tamaoki, Masanori/0000-0002-5516-8834 FU National Science Foundation [IOB-0444471, IOS-0817748]; Ministry of Education, Science, Sports and Culture of Japan [18780006] FX This work was supported by the National Science Foundation (grant nos. IOB-0444471 and IOS-0817748 to E. P. S.) and the Ministry of Education, Science, Sports and Culture of Japan (grant no. 18780006 to M. T.). NR 112 TC 70 Z9 75 U1 4 U2 47 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD AUG PY 2010 VL 153 IS 4 BP 1630 EP 1652 DI 10.1104/pp.110.156570 PG 23 WC Plant Sciences SC Plant Sciences GA 634FQ UT WOS:000280566000017 PM 20498337 ER PT J AU Famoso, AN Clark, RT Shaff, JE Craft, E McCouch, SR Kochian, LV AF Famoso, Adam N. Clark, Randy T. Shaff, Jon E. Craft, Eric McCouch, Susan R. Kochian, Leon V. TI Development of a Novel Aluminum Tolerance Phenotyping Platform Used for Comparisons of Cereal Aluminum Tolerance and Investigations into Rice Aluminum Tolerance Mechanisms SO PLANT PHYSIOLOGY LA English DT Article ID ORYZA-SATIVA L.; QUANTITATIVE-TRAIT LOCI; ORGANIC-ACIDS; RESISTANCE MECHANISMS; MOLECULAR MARKERS; CULTIVATED RICE; ROOT ELONGATION; ALMT1 GENE; ARABIDOPSIS; TOXICITY AB The genetic and physiological mechanisms of aluminum (Al) tolerance have been well studied in certain cereal crops, and Al tolerance genes have been identified in sorghum (Sorghum bicolor) and wheat (Triticum aestivum). Rice (Oryza sativa) has been reported to be highly Al tolerant; however, a direct comparison of rice and other cereals has not been reported, and the mechanisms of rice Al tolerance are poorly understood. To facilitate Al tolerance phenotyping in rice, a high-throughput imaging system and root quantification computer program was developed, permitting quantification of the entire root system, rather than just the longest root. Additionally, a novel hydroponic solution was developed and optimized for Al tolerance screening in rice and compared with the Yoshida's rice solution commonly used for rice Al tolerance studies. To gain a better understanding of Al tolerance in cereals, comparisons of Al tolerance across cereal species were conducted at four Al concentrations using seven to nine genetically diverse genotypes of wheat, maize (Zea mays), sorghum, and rice. Rice was significantly more tolerant than maize, wheat, and sorghum at all Al concentrations, with the mean Al tolerance level for rice found to be 2- to 6-fold greater than that in maize, wheat, and sorghum. Physiological experiments were conducted on a genetically diverse panel of more than 20 rice genotypes spanning the range of rice Al tolerance and compared with two maize genotypes to determine if rice utilizes the well-described Al tolerance mechanism of root tip Al exclusion mediated by organic acid exudation. These results clearly demonstrate that the extremely high levels of rice Al tolerance are mediated by a novel mechanism, which is independent of root tip Al exclusion. C1 [Clark, Randy T.; Shaff, Jon E.; Craft, Eric; Kochian, Leon V.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. [Famoso, Adam N.; McCouch, Susan R.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Kochian, LV (reprint author), Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. EM lvk1@cornell.edu OI Kochian, Leon/0000-0003-3416-089X FU U.S. Department of Agriculture Agricultural Food Research Institute [2009-02273] FX This work was supported by the U.S. Department of Agriculture Agricultural Food Research Institute (grant no. 2009-02273). NR 68 TC 61 Z9 72 U1 2 U2 34 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 J9 PLANT PHYSIOL JI Plant Physiol. PD AUG PY 2010 VL 153 IS 4 BP 1678 EP 1691 DI 10.1104/pp.110.156794 PG 14 WC Plant Sciences SC Plant Sciences GA 634FQ UT WOS:000280566000020 PM 20538888 ER PT J AU Wen, ZB Zhang, ML Zhu, GL Sanderson, SC AF Wen, Zhi-Bin Zhang, Ming-Li Zhu, Ge-Lin Sanderson, Stewart C. TI Phylogeny of Salsoleae s.l. (Chenopodiaceae) based on DNA sequence data from ITS, psbB-psbH, and rbcL, with emphasis on taxa of northwestern China SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Salsoleae s.l.; Anabasis; Cliniacoptera; Halogeton; Molecular phylogeny; China ID C-4 PHOTOSYNTHESIS; CHLOROPLAST DNA; EVOLUTION; SUBSTITUTION; COTYLEDONS; CONFIDENCE; BIENERTIA; INFERENCE; DESERTS; ANATOMY AB To reconstruct phylogeny and verify the monophyly of major subgroups, a total of 52 species representing almost all species of Salsoleae s.l. in China were sampled, with analysis based on three molecular markers (nrDNA ITS, cpDNA psbB psbH and thcL), using maximum parsimony, maximum likelihood, and Bayesian inference methods Our molecular evidence provides strong support for the following: (1) Camphorosmeae is nested within Salsoleae s.l. instead of the previously suggested sister relationship. (2) Tribe Salsoleae s.I is monophyletic and is composed of three monophyletic subunits, Caroxyloneae, the Kali clade, and Salsoleae s.str (3) Climacoptera is separated from Salsola s I It does not form a monophyletic group but is split into two monophyletic parts, Climacoptera I and Chmacoptera II (4) Halogeton is clearly polyphyletic, as are Anabasis and the genus Salsola s.l. (5) Caroxylon, Halo.xylon, Kali, and Petrosnnonia are well-supported monophyletic genera. Additional evidence is needed regarding the monophyly of Halimocnemis, which remains unclear. C1 [Wen, Zhi-Bin; Zhang, Ming-Li] Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China. [Wen, Zhi-Bin] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Zhang, Ming-Li] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Zhu, Ge-Lin] NW Normal Univ, Lanzhou 730070, Peoples R China. [Sanderson, Stewart C.] US Forest Serv, Shrub Sci Lab, Intermt Res Stn, USDA, Ogden, UT USA. RP Zhang, ML (reprint author), Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China. FU National Basic Research Program of China [2009CB825104]; Xinjiang Institute of Ecology and Geography; Chinese Academy of Sciences FX Thanks to Prof P. Yan for providing Salsoleae s l field collections from Xinjiang Province. China, Dr D M Williams (London, UK) for helpful comments on the manuscript, Mrs Lorraine Williams (London. UK) for improving the English of the manuscript, and two anonymous reviewers for valuable comments on a previous version. This research was funded by the National Basic Research Program of China (2009CB825104), Xinjiang Institute of Ecology and Geography. Chinese Academy of Sciences. NR 59 TC 21 Z9 23 U1 1 U2 21 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 EI 1615-6110 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD AUG PY 2010 VL 288 IS 1-2 BP 25 EP 42 DI 10.1007/s00606-010-0310-5 PG 18 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 641SU UT WOS:000281150800003 ER PT J AU Vougogiannopoulou, K Fokialakis, N Aligiannis, N Cantrell, C Skaltsounis, L AF Vougogiannopoulou, K. Fokialakis, N. Aligiannis, N. Cantrell, C. Skaltsounis, L. TI Novel indole alkaloids from Raputia simulans SO PLANTA MEDICA LA English DT Meeting Abstract CT 7th Tannin Conference/58th International Congress and Annual Meeting of the Society-for-Medical-Plant-and-Natural-Product-Research CY AUG 29-SEP 02, 2010 CL Berlin, GERMANY SP Soc Med Plant Nat Prod Res C1 [Vougogiannopoulou, K.; Fokialakis, N.; Aligiannis, N.; Skaltsounis, L.] Univ Athens, Fac Pharm Pharmacognosy & Nat Product Chem, GR-15771 Athens, Greece. [Cantrell, C.] ARS, USDA, Natl Ctr Nat Prod Res, Nat Prod Utilizat Res Unit, University, MS 38677 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2010 VL 76 IS 12 BP 1172 EP 1173 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 653DE UT WOS:000282066500037 ER PT J AU Baer-Dubowska, W Zielinska-Przyjemska, M Ignatowicz, E Rimando, A AF Baer-Dubowska, W. Zielinska-Przyjemska, M. Ignatowicz, E. Rimando, A. TI Effects of polyphenols: Resveratrol and its natural analogues and tannic acid on DNA oxidative damage and apoptosis in human neutrophils SO PLANTA MEDICA LA English DT Meeting Abstract CT 7th Tannin Conference/58th International Congress and Annual Meeting of the Society-for-Medical-Plant-and-Natural-Product-Research CY AUG 29-SEP 02, 2010 CL Berlin, GERMANY SP Soc Med Plant Nat Prod Res ID NF-KAPPA-B; MOUSE EPIDERMIS; COX-2; INOS C1 [Baer-Dubowska, W.; Zielinska-Przyjemska, M.; Ignatowicz, E.] Karol Marcinkowski Univ Med Sci, PL-60781 Poznan, Poland. [Rimando, A.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS USA. NR 2 TC 0 Z9 0 U1 1 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD AUG PY 2010 VL 76 IS 12 BP 1380 EP 1380 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 653DE UT WOS:000282066500796 ER PT J AU Liu, C Pitts, RJ Bohbot, JD Jones, PL Wang, GR Zwiebel, LJ AF Liu, Chao Pitts, R. Jason Bohbot, Jonathan D. Jones, Patrick L. Wang, Guirong Zwiebel, Laurence J. TI Distinct Olfactory Signaling Mechanisms in the Malaria Vector Mosquito Anopheles gambiae SO PLOS BIOLOGY LA English DT Article ID DROSOPHILA ODORANT RECEPTORS; GLUTAMATE RECEPTORS; MEMBRANE TOPOLOGY; AEDES-AEGYPTI; IDENTIFICATION; CHANNELS; BEHAVIOR; DEET AB Anopheles gambiae is the principal Afrotropical vector for human malaria, in which olfaction mediates a wide range of both adult and larval behaviors. Indeed, mosquitoes depend on the ability to respond to chemical cues for feeding, host preference, and mate location/selection. Building upon previous work that has characterized a large family of An. gambiae odorant receptors (AgORs), we now use behavioral analyses and gene silencing to examine directly the role of AgORs, as well as a newly identified family of candidate chemosensory genes, the An. gambiae variant ionotropic receptors (AgIRs), in the larval olfactory system. Our results validate previous studies that directly implicate specific AgORs in behavioral responses to DEET as well as other odorants and reveal the existence of at least two distinct olfactory signaling pathways that are active in An. gambiae. One system depends directly on AgORs; the other is AgOR-independent and requires the expression and activity of AgIRs. In addition to clarifying the mechanistic basis for olfaction in this system, these advances may ultimately enhance the development of vector control strategies, targeting olfactory pathways in mosquitoes to reduce the catastrophic effects of malaria and other mosquito-borne diseases. C1 [Liu, Chao; Pitts, R. Jason; Jones, Patrick L.; Wang, Guirong; Zwiebel, Laurence J.] Vanderbilt Univ, Dept Biol Sci, Ctr Mol Neurosci, Inst Biol Chem, Nashville, TN 37203 USA. [Liu, Chao; Pitts, R. Jason; Jones, Patrick L.; Wang, Guirong; Zwiebel, Laurence J.] Vanderbilt Univ, Dept Pharmacol, Ctr Mol Neurosci, Inst Biol Chem, Nashville, TN USA. [Liu, Chao; Pitts, R. Jason; Jones, Patrick L.; Wang, Guirong; Zwiebel, Laurence J.] Vanderbilt Univ, Dept Biol Sci, Ctr Mol Neurosci, Inst Global Hlth, Nashville, TN USA. [Liu, Chao; Pitts, R. Jason; Jones, Patrick L.; Wang, Guirong; Zwiebel, Laurence J.] Vanderbilt Univ, Dept Pharmacol, Ctr Mol Neurosci, Inst Global Hlth, Nashville, TN USA. [Liu, Chao; Pitts, R. Jason; Jones, Patrick L.; Wang, Guirong; Zwiebel, Laurence J.] Vanderbilt Univ, Program Dev Biol, Nashville, TN USA. [Bohbot, Jonathan D.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Inst Plant Sci,Invas Insect Biocontrol & Behav La, Beltsville, MD USA. RP Liu, C (reprint author), Vanderbilt Univ, Dept Biol Sci, Ctr Mol Neurosci, Inst Biol Chem, Nashville, TN 37203 USA. EM l.zwiebel@vanderbilt.edu OI Zwiebel, Laurence/0000-0001-6686-7289 FU Vanderbilt University; Foundation for the National Institutes of Health (NIH) through the Grand Challenges in Global Health Initiative (GCGH) [121]; NIH [AI056402] FX This work was funded by Vanderbilt University and by grants from the Foundation for the National Institutes of Health (NIH) through the Grand Challenges in Global Health Initiative (GCGH#121) and the NIH (AI056402) to LJZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 29 TC 48 Z9 51 U1 1 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1544-9173 J9 PLOS BIOL JI PLoS. Biol. PD AUG PY 2010 VL 8 IS 8 AR e1000467 DI 10.1371/journal.pbio.1000467 PG 17 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA 645MQ UT WOS:000281464500020 ER PT J AU Rugkong, A Rose, JKC Lee, SJ Giovannoni, JJ O'Neill, MA Watkins, CB AF Rugkong, Adirek Rose, Jocelyn K. C. Lee, Sang Jik Giovannoni, James J. O'Neill, Malcolm A. Watkins, Christopher B. TI Cell wall metabolism in cold-stored tomato fruit SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Solanum lycopersicum; Chilling injury; Fruit ripening; Cell walls; Polygalacturonase ID PECTOLYTIC ENZYME-ACTIVITY; POLYGALACTURONASE GENE; FLAVORTOP NECTARINES; TRANSGENIC TOMATOES; NEUTRAL POLYMERS; WOOLLY BREAKDOWN; MOLECULAR-WEIGHT; CHILLING INJURY; RIPENING FRUIT; PEACH FRUIT AB The effects of chilling on cell wall metabolism of tomato fruit (Solanum lycopersicum L. cv. Trust) have been investigated. Fruit were harvested at the breaker stage of maturity and ripened at 20 degrees C for 1-12 d, or stored at 3 degrees C for up to 3 weeks, and then ripened at 20 degrees C. The effects of cold storage on fruit ripening were small. Pericarp tissues from fruit stored for 2 weeks had only slightly reduced pectin solubilization and depolymerization. Polygalacturonase (PG) m RNA levels, PG protein accumulation and PG activity in the tissues were also reduced by chilling. A reduction of PG protein abundance and PG activity occurred to a greater extent than that of PG mRNA levels, suggesting that chilling affected post-transcriptional regulation. Expression of the expansin1 (LeEXP1) gene was also reduced by chilling, but LeExp1 protein accumulation levels were not affected by chilling. beta-Galactosiclase activity was highest in chilled fruit during cold storage and during early ripening, but expression of a beta-galactosidase gene (TBG4) was unaffected. While chilling had no effect on pectin methylesterase (PME) activity, expression of PME1 in tissues from cold-stored fruit was lower. Endo-beta-1,4-glucanase (EGase) activity and the expression of endo-beta-1,4-glucanase (Cel1) were not affected by chilling. The predominant effect of chilling on the activity, protein accumulation, and gene expression of PG did not correlate with pectin solubilization and depolymerization. (C) 2010 Elsevier B.V. All rights reserved. C1 [Rugkong, Adirek; Watkins, Christopher B.] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Rose, Jocelyn K. C.; Lee, Sang Jik] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Giovannoni, James J.] USDA ARS, Ithaca, NY 14853 USA. [Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [O'Neill, Malcolm A.] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA. [O'Neill, Malcolm A.] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA. RP Watkins, CB (reprint author), Prince Songkla Univ, Dept Plant Sci, Fac Nat Resources, Hat Yai 90112, Songkhla, Thailand. EM cbw3@cornell.edu FU Cornell University Agricultural Experiment Station [NE-1036]; Cooperative State Research, Education and Extension Service, U.S. Department of Agriculture; Department of Horticulture FX This research was supported by the Cornell University Agricultural Experiment Station, federal formula funds, Project NE-1036, received from the Cooperative State Research, Education and Extension Service, U.S. Department of Agriculture. A.R. was supported by a Department of Horticulture Assistantship. NR 45 TC 18 Z9 20 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD AUG PY 2010 VL 57 IS 2 BP 106 EP 113 DI 10.1016/j.postharvbio.2010.03.004 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA 614UO UT WOS:000279085500005 ER PT J AU Gast, RK Jones, DR Anderson, KE Guraya, R Guard, J Holt, PS AF Gast, R. K. Jones, D. R. Anderson, K. E. Guraya, R. Guard, J. Holt, P. S. TI In vitro penetration of Salmonella Enteritidis through yolk membranes of eggs from 6 genetically distinct commercial lines of laying hens SO POULTRY SCIENCE LA English DT Article DE Salmonella Enteritidis; egg; yolk; vitelline membrane; penetration ID ENTERICA SEROVAR ENTERITIDIS; EXPERIMENTALLY INFECTED HENS; INOCULATED SHELL EGGS; VITELLINE MEMBRANE; UNITED-STATES; PHAGE TYPE-4; MICROBIAL-CONTAMINATION; SEROTYPE ENTERITIDIS; RISK-ASSESSMENT; CHICKEN LINES AB Although deposition of Salmonella Enteritidis inside yolks is less common than deposition in albumen or on the vitelline (yolk) membrane in naturally contaminated eggs laid by infected hens, bacterial migration into the yolk to reach its nutrient-rich contents could lead to extensive multiplication. The present study used an in vitro egg contamination model to assess the ability of small initial numbers of Salmonella Enteritidis to penetrate the vitelline membrane and multiply inside yolks of eggs laid by 6 genetically distinct commercial lines of hens during 24 h of storage at 30 C. Eggs from each line were tested at 4 different hen ages by inoculation of approximately 100 cfu of Salmonella Enteritidis onto the outside of the vitelline membranes of intact yolks in plastic centrifuge tubes and then adding back the albumen into each tube before incubation. Overall, the frequency of penetration of Salmonella Enteritidis into the yolk contents of eggs from individual lines of hens ranged from 30 to 58% and the mean concentration of Salmonella Enteritidis in yolk contents after incubation ranged from 0.8 to 2.0 log10 cfu/mL. For both of these parameters, values for one hen line were significantly higher than for 2 other lines, but no other differences were observed. Hen age did not have a significant effect on egg yolk penetration by Salmonella Enteritidis. These results indicate that opportunities for the migration and growth of small initial numbers of Salmonella Enteritidis to attain more dangerous levels inside contaminated eggs during storage at warm temperatures can sometimes vary between different lines of laying hens. C1 [Gast, R. K.; Jones, D. R.; Guraya, R.; Guard, J.; Holt, P. S.] ARS, USDA, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Anderson, K. E.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC USA. RP Gast, RK (reprint author), ARS, USDA, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. EM Richard.Gast@ars.usda.gov NR 45 TC 2 Z9 2 U1 0 U2 2 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG 1 PY 2010 VL 89 IS 8 BP 1732 EP 1736 DI 10.3382/ps.2009-00440 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA 626AN UT WOS:000279938100023 PM 20634530 ER PT J AU Zhuang, H Savage, EM Lawrence, K AF Zhuang, H. Savage, E. M. Lawrence, K. TI Effect of 3 postmortem electrical stimulation treatments on the quality of early deboned broiler breast meat SO POULTRY SCIENCE LA English DT Article DE broiler; electrical stimulation; breast meat; quality; shear force ID WARNER-BRATZLER; SENSORY TENDERNESS; SHEAR VALUES; ALLO-KRAMER; TEXTURE; FILLETS; PECTORALIS; MUSCLE; CHICKENS; POULTRY AB The present experiment was carried out to evaluate the effects of electrical stimulation (ES) immediately prescalding (PS), ES immediately postdefeathering (PD), or PS combined with PD (PSPD) on the quality of early deboned (2 h) broiler breast muscles, pectoralis major (fillets), and pectoralis minor (tenders). No stimulation, early-deboned (2 h), and 24-h deboned (24 h) fillets were used for the comparison. The 42-d-old broiler carcasses were electrically stimulated with pulsed current at 200 V for 30 s over a 90-s time interval (total of 1 min over 180 s for PSPD), and breast meat was deboned 2 h postmortem. Quality indicators evaluated were CIE L*, a*, and b* color and pH of the raw fillets and cook yields and Warner-Bratzler (WB) shear force of the fillets and tenders. There were no differences in raw fillet color, pH, and cook yields of both the fillets and tenders between the 3 ES treatments. Effects of different ES treatments on meat WB shear force values varied with breast muscles. For the fillets, the average WB shear force values of both the PS and PSPD samples, which were not different from each other, were significantly lower than those of the PD samples. For the tenders, there were no differences in the average shear force values between the 3 ES treatments. Regardless of ES treatment and breast muscle, early deboned broiler breast meat from ES carcasses required significantly less force to shear than the 2-h control. These results indicate that ES can tenderize early deboned poultry breast muscles; however, the effectiveness of ES tenderization varies with ES treatments for the fillets. The PS treatment is more effective in reducing fillet shear values than PD, and there is no further reduction in shear values with PSPD compared with the PS treatment. C1 [Zhuang, H.; Savage, E. M.; Lawrence, K.] ARS, Qual & Safety Assessment Res Unit, USDA, Richard B Russell Res Ctr, Athens, GA 30604 USA. RP Zhuang, H (reprint author), ARS, Qual & Safety Assessment Res Unit, USDA, Richard B Russell Res Ctr, POB 5677, Athens, GA 30604 USA. EM hong.zhuang@ars.usda.gov NR 32 TC 0 Z9 0 U1 2 U2 9 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG 1 PY 2010 VL 89 IS 8 BP 1737 EP 1743 DI 10.3382/ps.2009-00460 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 626AN UT WOS:000279938100024 PM 20634531 ER PT J AU Bowker, BC Callahan, JA Solomon, MB AF Bowker, B. C. Callahan, J. A. Solomon, M. B. TI Effects of hydrodynamic pressure processing on the marination and meat quality of turkey breasts SO POULTRY SCIENCE LA English DT Article DE turkey breast; hydrodynamic pressure processing; marination; meat quality ID SENSORY CHARACTERISTICS; OXIDATIVE STABILITY; LONGISSIMUS MUSCLE; CHICKEN PARTS; TENDERNESS; TENDERIZATION; INJECTION; BINDING; SALT AB The effects of hydrodynamic pressure processing (HDP) on marination and meat quality characteristics of turkey breasts were investigated. Breast muscles from 45 turkey hens were removed from the carcasses within 30 min postmortem. From each bird, the breast from one side was treated with HDP and the other side served as a nontreated control. Breasts were then marinated in either 15 or 30% brine (water, salt, and phosphate) based on muscle weight with vacuum tumbling for 30 min or nonmarinated. The control and HDP-treated breasts from each bird received the same marination treatment. Brine uptake, processing yield, and cooking loss were measured as processing characteristics and texture, color, and expressible moisture were measured to document changes in meat quality. Hydrodynamic pressure processing increased (P < 0.001) brine uptake after 10 and 30 min of marination and increased (P < 0.001) processing yield compared with controls. The HDP-induced improvements in these processing characteristics were augmented at 30% brine levels compared with 15% brine. Cooking loss was lower (P < 0.001) in marinated breasts compared with nonmarinated samples. Hydrodynamic pressure processing decreased (P < 0.0001) Warner-Bratzler shear force and significantly influenced texture profile parameters, resulting in reduced hardness but increased cohesiveness and springiness compared with controls at both marination levels. Hydrodynamic pressure processing did not influence color (L*, a*, and b*) or expressible moisture values compared with controls at either marination level. Marinated samples (15 and 30% brine levels) had lower (P < 0.001) Warner-Bratzler shear force values and lower (P < 0.05) hardness, cohesiveness, and chewiness values compared with nonmarinated samples. Data from this study suggest that HDP enhances brine absorption, increases processing yield, and improves texture characteristics in marinated turkey breasts. C1 [Bowker, B. C.; Callahan, J. A.; Solomon, M. B.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Bowker, BC (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM brian.bowker@ars.usda.gov NR 29 TC 8 Z9 8 U1 0 U2 12 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD AUG 1 PY 2010 VL 89 IS 8 BP 1744 EP 1749 DI 10.3382/ps.2009-00484 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 626AN UT WOS:000279938100025 PM 20634532 ER PT J AU Svetoch, EA Stern, NJ AF Svetoch, E. A. Stern, N. J. TI Bacteriocins to control Campylobacter spp. in poultry - A review SO POULTRY SCIENCE LA English DT Review DE Campylobacter; bacteriocin; colonization; broiler; intervention ID LACTIC-ACID BACTERIA; ANTIMICROBIAL PEPTIDES; FOOD PRESERVATION; JEJUNI; NISIN; BIOSYNTHESIS; COLONIZATION; PURIFICATION; ANTIBIOTICS; INHIBITION AB The unacceptably high frequency of Campylobacter jejuni transmission from poultry to humans encourages scientists to consider and create alternative intervention strategies to control the pathogen in poultry production. Extremely high numbers of Campylobacter (often > 10(8) cfu/g of poultry intestinal material) potentiate high numbers of the organism on the processed broiler carcass with increasing consequent human health risk. Many scientists believe interventions during poultry production portend the greatest opportunity for reducing risk of disease. Over the past 10 yr, we have focused our studies on nonantibiotic bacteriocin application to intervene during animal production and this is the subject of the current review. The application of therapeutic bacteriocin treatments to reduce poultry colonization diminishes Campylobacter from > 10(8) cfu/g of cecal materials to nondetectable or very low levels in treated birds. Further, the review provides scientists with a useful starting point for the further development of industry-applicable interventions leading to reduced transmission of this agent in human disease. C1 [Stern, N. J.] ARS, USDA, Athens, GA 30605 USA. [Svetoch, E. A.] State Res Ctr Appl Microbiol & Biotechnol, Moscow, Russia. RP Stern, NJ (reprint author), ARS, USDA, Athens, GA 30605 USA. EM norman.stern@ars.usda.gov FU U.S. State Department through the International Science and Technology Center; ARS Office of International Research Program [1720, 3445] FX Funds for the conduct of the studies conducted by USDA-ARS and SRCAMB were provided by the U.S. State Department through the International Science and Technology Center and the ARS Office of International Research Programs projects #1720 and #3445. NR 46 TC 38 Z9 38 U1 3 U2 34 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG 1 PY 2010 VL 89 IS 8 BP 1763 EP 1768 DI 10.3382/ps.2010-00659 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 626AN UT WOS:000279938100028 PM 20634535 ER PT J AU Hingle, MD O'Connor, TM Dave, JM Baranowski, T AF Hingle, Melanie D. O'Connor, Teresia M. Dave, Jayna M. Baranowski, Tom TI Parental involvement in interventions to improve child dietary intake: A systematic review SO PREVENTIVE MEDICINE LA English DT Review DE Children; Youth; Interventions; Diet; Parents; Obesity prevention ID RANDOMIZED CONTROLLED-TRIAL; OBESITY PREVENTION PROGRAMS; PHYSICAL-ACTIVITY; VEGETABLE CONSUMPTION; CARDIOVASCULAR RISK; PRESCHOOL-CHILDREN; CONSORT STATEMENT; NUTRITION INTERVENTION; OUTCOME EVALUATION; US CHILDREN AB Objective. Interventions that aim to improve child dietary quality and reduce disease risk often involve parents. The most effective methods to engage parents remain unclear. A systematic review of interventions designed to change child and adolescent dietary behavior was conducted to answer whether parent involvement enhanced intervention effectiveness, and what type of involvement was most effective in achieving desired outcomes. Method. In 2008, Pub Med, Medline, Psych Info, and Cochrane Library databases were searched to identify programs designed to change child and adolescent dietary intake that also involved parents. Methods of parental involvement were categorized based on the type and intensity of parental involvement. These methods were compared against intervention design, dietary outcomes, and quality of reporting (evaluated using CONSORT checklist) for each study. Results. The literature search identified 1774 articles and 24 met review criteria. Four studies systematically evaluated parent involvement with inconsistent results. Indirect methods to engage parents were most commonly used, although direct approaches were more likely to result in positive outcomes. Four studies met >70% of CONSORT items. Conclusion. Limited conclusions may be drawn regarding the best method to involve parents in changing child diet to promote health. However, direct methods show promise and warrant further research. (C) 2010 Elsevier Inc. All rights reserved. C1 [Hingle, Melanie D.] Univ Arizona, Dept Nutr Sci, Tucson, AZ 85721 USA. [O'Connor, Teresia M.; Dave, Jayna M.; Baranowski, Tom] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Hingle, MD (reprint author), Univ Arizona, Dept Nutr Sci, Shantz Bldg,Room 309,1177 E 4th St, Tucson, AZ 85721 USA. EM hinglem@email.arizona.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Child Health and Human Development Training [5T32HD007445]; USDA/ARS [58-6250-6001] FX This research was funded by a National Institute of Child Health and Human Development Training Grant 5T32HD007445, "Research Training in Maternal, Infant & Child Nutrition." This work is also a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and has been funded in part with federal funds from the USDA/ARS under Cooperative Agreement no. 58-6250-6001. The contents of this publication do not necessarily reflect the views or policies of the USDA nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. NR 68 TC 77 Z9 77 U1 2 U2 27 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0091-7435 J9 PREV MED JI Prev. Med. PD AUG PY 2010 VL 51 IS 2 BP 103 EP 111 DI 10.1016/j.ypmed.2010.04.014 PG 9 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA 632UL UT WOS:000280454300001 PM 20462509 ER PT J AU Bannantine, JP Stabel, JR Bayles, DO Geisbrecht, BV AF Bannantine, John P. Stabel, Judith R. Bayles, Darrell O. Geisbrecht, Brian V. TI Characteristics of an extensive Mycobacterium avium subspecies paratuberculosis recombinant protein set SO PROTEIN EXPRESSION AND PURIFICATION LA English DT Article DE Proteins; Paratuberculosis; Johne's disease ID ANTIGEN DISCOVERY; PURIFICATION; ANTIBODY; IDENTIFICATION; EXPRESSION; INFECTION; DISEASE AB In the first step of a comprehensive large-scale antigen discovery project, 651 genes of Mycobacterium avium subspecies paratuberculosis were expressed in Escherichia coli. All of these were purified by affinity chromatography, dialyzed in phosphate buffered saline, and analyzed on SOS-PACE gels. Collectively, these purified recombinant proteins represent 14.9% of the total M. avium subsp. paratuberculosis proteome. This volume of protein expression and purification has yielded unique observations that may be missed in smaller scale expression and purification projects. For example, the 252 putative membrane proteins predicted by PSORTb analysis, resulted in lower average expression yields (3.51 mg/l culture) than the 176 predicted cytoplasmic proteins (7.27 mg/l culture). A few proteins (MAP0107c, MAP3169c and MAP3640) appear to promote lysis of E. coli since there was a drop in optical density of the growth culture minutes after the inducing agent was added. Certain M. avium subsp. paratuberculosis proteins, when expressed in E. coli changed the color of the column resin or appearance of harvested cell pellets. Finally, 19 proteins showed an absorbance maximum at 260 nm rather than 280 nm that was attributed to binding of nucleic acid during purification. This extensive recombinant protein repository provides a powerful tool for proteome- and genome-scale research of this organism. Published by Elsevier Inc. C1 [Bannantine, John P.; Stabel, Judith R.; Bayles, Darrell O.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Geisbrecht, Brian V.] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA. RP Bannantine, JP (reprint author), USDA ARS, Natl Anim Dis Ctr, 2300 N Dayton Ave, Ames, IA 50010 USA. EM john.bannantine@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 FU USDA; Missouri Life Sciences Research Board [13238] FX The expert technical assistance of Janis Hansen is gratefully acknowledged. This study was supported by the USDA-Agricultural Research Service. Portions of this work were also funded by USDA-CSREES-CAP project entitled Johne's disease integrated program (JDIP) to J.P.B. and the Missouri Life Sciences Research Board (#13238) to B.V.G. NR 31 TC 13 Z9 14 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1046-5928 J9 PROTEIN EXPRES PURIF JI Protein Expr. Purif. PD AUG PY 2010 VL 72 IS 2 BP 223 EP 233 DI 10.1016/j.pep.2010.03.019 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 603FD UT WOS:000278193200012 PM 20359537 ER PT J AU Pechanova, O Pechan, T Ozkan, S McCarthy, FM Williams, WP Luthe, DS AF Pechanova, Olga Pechan, Tibor Ozkan, Seval McCarthy, Fiona M. Williams, W. Paul Luthe, Dawn S. TI Proteome profile of the developing maize (Zea mays L.) rachis SO PROTEOMICS LA English DT Article DE 2-DE; 2-D LC-MS/MS; Plant proteomics; Rachis; Zea mays ID LISTERIA-MONOCYTOGENES; PROTEINS; IDENTIFICATION; SOLUBILIZATION; RESOURCE; GENOMES AB In this study, we performed the first high-throughput proteomic analysis of developing rachis (cob) from maize genotype Mp313E. Using two proteomic approaches, 2-DE and 2-D LC, we identified 967 proteins. A 2-D proteome reference map was established. Functional classification of identified proteins revealed that proteins involved in various cellular metabolisms, response to stimulus and transport, were the most abundant. C1 [Pechan, Tibor] Mississippi State Univ, Life Sci & Biotechnol Inst, Mississippi State, MS 39762 USA. [Pechanova, Olga] Mississippi State Univ, Dept Biochem & Mol Biol, Mississippi State, MS 39762 USA. [Ozkan, Seval] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA. [McCarthy, Fiona M.] Mississippi State Univ, Coll Vet Med, Mississippi State, MS 39762 USA. [Williams, W. Paul] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS USA. [Luthe, Dawn S.] Penn State Univ, Dept Crop & Soil Sci, University Pk, PA 16802 USA. RP Pechan, T (reprint author), Mississippi State Univ, Life Sci & Biotechnol Inst, Mississippi State, MS 39762 USA. EM pechan@ra.msstate.edu FU USDA-ARS; Mississippi Agricultural and Forestry Experiment Station [SCA 58-6406-6-039] FX The authors thank Dr. Shane Burgess, Dr. Susan Bridges and Amanda Cooksey for their valuable advice and technical assistance. Mass spectrometry was conducted at Life Sciences and Biotechnology Institute at Mississippi State University. This research was funded by a specific cooperative agreement between USDA-ARS and the Mississippi Agricultural and Forestry Experiment Station (SCA 58-6406-6-039). Approved for publication as Journal Article No J11737 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University. NR 19 TC 16 Z9 16 U1 0 U2 5 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1615-9853 J9 PROTEOMICS JI Proteomics PD AUG PY 2010 VL 10 IS 16 BP 3051 EP 3055 DI 10.1002/pmic.200900833 PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 645TB UT WOS:000281488000015 PM 20662101 ER PT J AU Garcia-Bocanegra, I Dubey, JP Simon-Grife, M Cabezon, O Casal, J Allepuz, A Napp, S Almeria, S AF Garcia-Bocanegra, I. Dubey, J. P. Simon-Grife, M. Cabezon, O. Casal, J. Allepuz, A. Napp, S. Almeria, S. TI Seroprevalence and risk factors associated with Toxoplasma gondii infection in pig farms from Catalonia, north-eastern Spain SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Toxoplasma gondii; Pig; Seroprevalence; Risk factors; Catalonia, Spain ID TRICHINELLA-SPIRALIS; SOWS; ANTIBODIES; SYSTEMS; SWINE; ILLINOIS; ANIMALS; HUMANS; CATS AB Seroprevalence and associated risk factors for Toxoplasma gondii infection in pigs were analyzed in 1202 sera samples, including sows and pigs of three, seven, 11, 15 and 20 weeks of age, from 23 farms in Catalonia, north-eastern Spain. Antibodies were tested by the modified agglutination test (MAT) at titers >= 1:25. Antibodies to T. gondii were found in 228 samples (19.0%; 95% Cl: 16.8-21.2). The individual prevalence in animals higher than 7 weeks of age was 22.8% (174/762; 95% Cl: 16.6-29.0) and the within-farm prevalence ranged from 7.1% to 36.4%. Statistically significant differences were found among age classes. The risk factors significantly associated with T. gondii seroprevalence were the presence of cats, percentage of mortality at weaning and the presence of outdoor facilities in the farms. The seroprevalence observed in the present study indicates widespread exposure to T. gondii among domestic pigs in Catalonia, which may have important implications for public health. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Garcia-Bocanegra, I.; Simon-Grife, M.; Cabezon, O.; Casal, J.; Allepuz, A.; Napp, S.; Almeria, S.] UAB IRTA, CReSA, Barcelona 08193, Spain. [Dubey, J. P.] USDA ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA. [Cabezon, O.] Univ Autonoma Barcelona, SEFaS, Dept Med & Cirurgia Anim, E-08193 Barcelona, Spain. [Casal, J.; Allepuz, A.; Almeria, S.] Univ Autonoma Barcelona, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain. RP Almeria, S (reprint author), UAB IRTA, CReSA, Campus Univ Autonoma Barcelona, Barcelona 08193, Spain. EM Sonia.Almeria@uab.cat RI Cabezon, Oscar/L-3812-2014; Allepuz, Alberto/N-2398-2014; Almeria, Sonia/N-4663-2014; Casal, Jordi/F-3020-2016 OI Cabezon, Oscar/0000-0001-7543-8371; Allepuz, Alberto/0000-0003-3518-1991; Almeria, Sonia/0000-0002-0558-5488; Casal, Jordi/0000-0002-6909-9366 FU CONSOLIDER-INGENIO 2010 PORCIVIR FX This study was supported by project CONSOLIDER-INGENIO 2010 PORCIVIR. NR 20 TC 15 Z9 16 U1 0 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 J9 RES VET SCI JI Res. Vet. Sci. PD AUG PY 2010 VL 89 IS 1 BP 85 EP 87 DI 10.1016/j.rvsc.2010.01.017 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA 609NF UT WOS:000278662800014 PM 20189210 ER PT J AU Hoberg, EP AF Hoberg, E. P. TI Invasive processes, mosaics and the structure of helminth parasite faunas SO REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES LA English DT Article DE Anthropogenic event; Biodiversity; Helminth; Invasive species; Propagule; Vertebrate parasite ID EMERGING INFECTIOUS-DISEASES; CLIMATE-CHANGE IMPACTS; N-SP NEMATODA; NORTH-AMERICA; BIOLOGICAL INVASIONS; ENVIRONMENTAL-CHANGE; SPICULOPTERAGIA-SPICULOPTERA; HISTORICAL BIOGEOGRAPHY; HAEMONCHUS-CONTORTUS; POPULATION-GENETICS AB The biosphere in evolutionary and ecological time has been structured by episodes of geographic and host colonisation that have determined distributions of complex assemblages of microparasites and macroparasites, including helminths circulating among vertebrates. Biological invasion is an intricate phenomenon often involving 'extra-range dispersal' and establishment of exotic (non-indigenous) species and populations substantially beyond their native range. Invasion may also involve the expansion or shifting of host and geographic distributions of an endemic (indigenous) species or fauna under changing environmental conditions. Invasions result in faunal interchange occurring under influences from both natural and anthropogenic forces where expansion on spatial/temporal continua bridges continents, regions and landscapes. Drivers for invasion are idiosyncratic, multifactorial, interactive, and opportunistic, with a powerful role for historical contingency. The life history patterns of helminths interact with invasion pathways to determine the potential for introduction. Human-mediated events, such as the global expansion of pathogens linked to development of agriculture, domestication of food animals, and European exploration have had a pervasive influence on the distribution of helminths. Globalisation, broad transport networks and environmental perturbation linked to climate change, along with other drivers, have accelerated these processes. A consequence of invasion and establishment of exotic species is that faunal structure will be a mosaic that includes admixtures of indigenous and non-indigenous species and populations; exemplified by helminth faunas among domestic and free-ranging ungulates and a diversity of host-parasite systems among vertebrates. Contemporary mosaics are evident where human-mediated events have brought assemblages of new invaders and relatively old endemic species into sympatry, highlighting interactions at ecotones, particularly those at borderlands between managed and natural ecosystems. Understanding the historical origins and complex components of mosaics is essential in formulating predictions about future responses to environmental change. Powerful tools are available which support the study of invasive species, the most important being systematics and our capacity to accurately identify parasites and to define evolutionary and biogeographic history. Faunal baselines derived from arrays of biological specimens, integrated surveys and informatics are a permanent record of the biosphere when archived in museum collections. The absence of comprehensive taxonomic inventories of parasites, including molecular-genetic data, limits our ability to recognise the introduction of non-indigenous parasites, and to document patterns of expansion for local faunas under a regime of environmental perturbation. C1 ARS, US Natl Parasite Collect, USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Hoberg, EP (reprint author), ARS, US Natl Parasite Collect, USDA, Anim Parasit Dis Lab, BARC E 1180,10100 Baltimore Ave, Beltsville, MD 20705 USA. EM Eric.Hoberg@ars.usda.gov FU National Science Foundation [DEB 0196095, 0415668] FX I extend my appreciation to D.R. Brooks, Department of Zoology, University of Toronto; B.M. Rosenthal, Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Beltsville; J.A. Cook, Department of Biology, University of New Mexico; and members of the Research Group for Arctic Parasitology, including S.J. Kutz, Faculty of Veterinary Medicine, University of Calgary, and L. Polley and E.J. Jenkins, Western College of Veterinary Medicine, University of Saskatchewan, Canada for collaborations and discussions which contributed to this manuscript. The current paper is a contribution (in part) from the Beringian Coevolution Project (with J.A. Cook) exploring historical biogeography and evolution of complex parasite systems across the roof of the world (National Science Foundation, DEB 0196095 and 0415668). NR 116 TC 31 Z9 34 U1 1 U2 20 PU OFFICE INT EPIZOOTIES PI PARIS PA 12 RUE DE PRONY, 75017 PARIS, FRANCE SN 0253-1933 J9 REV SCI TECH OIE JI Rev. Sci. Tech. Off. Int. Epizoot. PD AUG PY 2010 VL 29 IS 2 BP 255 EP 272 PG 18 WC Veterinary Sciences SC Veterinary Sciences GA 645TH UT WOS:000281488600006 PM 20919581 ER PT J AU Rossi, FJ Carter, DR Alavalapati, JRR Nowak, JT AF Rossi, Frederick J. Carter, Douglas R. Alavalapati, Janaki R. R. Nowak, John T. TI Forest Landowner Participation in State-Administered Southern Pine Beetle Prevention Cost-Share Programs SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE healthy forest management; financial incentive; southern pine beetle infestations; landowner survey AB Healthy pine trees in low-density stands offer the best defense against the southern pine beetle (SPB), helping to ensure that timber resources and other benefits of forests are protected against infestations. Through the SPB prevention cost-share program, landowners of nonindustrial private forestland are able to receive a financial incentive for improving forest health by proactively undertaking forest management practices. In this study, two surveys were used to analyze this program: (1) a survey of enrollees in the SPB prevention cost-share program, and (2) a survey of forest landowners who have not participated in a cost-share program. Data are used to examine similarities and differences in the two samples (e.g., background awareness of the SPB, sources of their information about the SPB). Information obtained from cost-share program enrollees is also presented to characterize their participation and to provide an overall evaluation of the program. Data indicate that the SPB prevention cost-share program is very successful in terms of the satisfaction of its customers (i.e., the actual program participants). C1 [Rossi, Frederick J.; Carter, Douglas R.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Alavalapati, Janaki R. R.] Virginia Polytech Inst & State Univ, Dept Forestry, Blacksburg, VA 24061 USA. [Nowak, John T.] US Forest Serv, Asheville, NC 28804 USA. RP Rossi, FJ (reprint author), Univ Florida, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. EM frossi@ufl.edu NR 12 TC 6 Z9 6 U1 1 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2010 VL 34 IS 3 BP 110 EP 117 PG 8 WC Forestry SC Forestry GA 634GO UT WOS:000280568600002 ER PT J AU Randolph, KC AF Randolph, KaDonna C. TI Equations Relating Compacted and Uncompacted Live Crown Ratio for Common Tree Species in the South SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE Forest Inventory and Analysis; crown base height; crown modeling; nonlinear regression; forest inventory AB Species-specific equations to predict uncompacted crown ratio (UNCR) from compacted live crown ratio ((CCR), tree length, and stem diameter were developed for 24 species and 12 genera in the southern United States. Using data from the US Forest Service Forest Inventory and Analysis program, nonlinear regression was used to model UNCR with a logistic function. Model performance was evaluated with standard fit statistics (root mean squared error, mean absolute error, mean error, and model efficiency) and by comparing the results of using the observed and predicted UNCR values in secondary applications. Root mean squared error for the regression models ranged from 0.062 to 0.176 UNCR and averaged 0.114 UNCR across all models. Height to live crown base calculations and crown width estimations based on the observed and predicted UNCR values were in close agreement. Overall, the models performed well for the Pinus and Taxodium genera and several individual hardwood species; however, model performance was generally poor for the Ater, Quartos, and Carp genera. C1 US Forest Serv, So Res Stn FIA, Knoxville, TN 37919 USA. RP Randolph, KC (reprint author), US Forest Serv, So Res Stn FIA, 4700 Old Kingston Pike, Knoxville, TN 37919 USA. EM krandolph@fs.fed.us NR 16 TC 4 Z9 4 U1 0 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2010 VL 34 IS 3 BP 118 EP 123 PG 6 WC Forestry SC Forestry GA 634GO UT WOS:000280568600003 ER PT J AU Roesch, FA Van Deusen, PC AF Roesch, Francis A. Van Deusen, Paul C. TI Anomaly Detection for Analysis of Annual Inventory Data: A Quality Control Approach SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE sampling; control charts ID FOREST INVENTORY AB Annual forest inventories present special challenges and opportunities for those analyzing the data arising from them. Here, we address one question currently being asked by analysts of the US Forest Service's Forest Inventory and Analysis Program's quickly accumulating annual inventory data. The question is simple but profound: When combining the next year's data for a particular variable with data from previous years, how does one know whether the same model as used in the past for this purpose continues to be applicable? Of the myriad approaches that have been developed for changepoint detection and anomaly detection, this report focuses on a simple quality-control approach known as a control chart that will allow analysts of annual forest inventory data to determine when a departure from a past trend is likely to have occurred. C1 [Roesch, Francis A.] US Forest Serv, So Res Stn, Asheville, NC 28804 USA. [Van Deusen, Paul C.] Natl Council Air & Stream Improvement, Stat & Model Dev Grp, Lowell, MA 01854 USA. RP Roesch, FA (reprint author), US Forest Serv, So Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM froesch@fs.fed.us NR 17 TC 0 Z9 0 U1 1 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2010 VL 34 IS 3 BP 131 EP 137 PG 7 WC Forestry SC Forestry GA 634GO UT WOS:000280568600005 ER PT J AU Eckhardt, L Sayer, MAS Imm, D AF Eckhardt, Lori Sayer, Mary Anne Sword Imm, Don TI State of Pine Decline in the Southeastern United States SO SOUTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE Leptographium; loblolly die-off; off-site; root disease; resource stress ID LOBLOLLY-PINE; HYDRAULIC REDISTRIBUTION; GROWTH; COLEOPTERA; SCOLYTIDAE; STANDS; WATER AB Pine decline is an emerging forest health issue in the southeastern United States. Observations suggest pine decline is caused by environmental stress arising from competition, weather, insects and fungi, anthropogenic disturbances, and previous management. The problem is most severe for loblolly pine on sites that historically supported longleaf pine, are highly eroded, or are not managed. The purposes of this technical note are (1) to describe the symptomology and extent of pine decline in the southeastern United States; (2) to describe its connection with root disease, resource stress, and silviculture; and (3) to summarize the consensus opinion of scientists and land managers during a workshop sponsored by the US Army Strategic Environmental Research and Development Program regarding the scope of this syndrome and the best research avenues to counter its potential effect on the sustainability of southern pine forests. C1 [Eckhardt, Lori] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Sayer, Mary Anne Sword] US Forest Serv, So Res Stn, Pineville, LA 71360 USA. [Imm, Don] US Fish & Wildlife Serv, Panama City, FL 32405 USA. RP Eckhardt, L (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 602 Duncan Dr,Suite 3301, Auburn, AL 36849 USA. EM eckhalg@auburn.edu NR 22 TC 8 Z9 8 U1 3 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0148-4419 J9 SOUTH J APPL FOR JI South. J. Appl. For. PD AUG PY 2010 VL 34 IS 3 BP 138 EP 141 PG 4 WC Forestry SC Forestry GA 634GO UT WOS:000280568600006 ER PT J AU Ames, M Spooner, DM AF Ames, Mercedes Spooner, David M. TI Phylogeny of Solanum series Piurana and related species in Solanum section Petota based on five conserved ortholog sequences SO TAXON LA English DT Article DE COSII markers; phylogeny; Solanaceae; Solanum; wild potatoes ID RESTRICTION SITE VARIATION; WILD POTATOES; POLYPLOIDY; EVOLUTION; SOLANACEAE; ORIGINS; REEXAMINATION; INFERENCE; TOMATOES; PLANTS AB Taxonomic complexity in wild potatoes (Solanum L. sect. Petota Dumort.) has led to widely conflicting treatments. Solanum ser. Piurana Hawkes is one of 21 series recognized in S. sect. Petota in the latest comprehensive taxonomic treatment by Hawkes in 1990. They are distributed from southern Colombia south through Ecuador to central Peru. The taxonomic limits of the series and validity of its constituent species were not resolved with previous studies. In the present study, a set of five conserved orthologous DNA markers (cos II) with 5342 bp of aligned length were used to infer the phylogeny of putative members of Solanum ser. Piurana and outgroups. The results agreed with a three-clade topology shown by previous studies within S. sect. Petota. Solomon ser. Piurana is expanded to include some species formerly included in S. sers. Conicibaccata, Cuneoalata, Ingaefolia, Olmosiana, Simplicissima, Tuberosa and Yungasensa. This expanded group is supported morphologically by the presence of moniliform tubers and coriaceous leaves in most species. C1 [Spooner, David M.] Univ Wisconsin, USDA, Agr Res Serv, Vegetable Crops Res Unit,Dept Hort, Madison, WI 53706 USA. [Ames, Mercedes] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. RP Spooner, DM (reprint author), Univ Wisconsin, USDA, Agr Res Serv, Vegetable Crops Res Unit,Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov FU USDA; NSF [DEB 0316614]; USDA National Research Initiative [2008-35300-18669] FX This work represents partial fulfillment for a Ph.D. Degree in Plant Breeding and Plant Genetics by M. A. at the University of Wisconsin-Madison. We thank committee members Eve Emshwiller, Michael Havey, Shelley Jansky, and Kenneth Sytsma; Flor Rodriguez for advice on data analysis and William Roca and Alberto Salas for help in the morphological studies in Peru; Holly Ruess for technical assistance; and the staff of the CIP and US potato genebanks for help in obtaining germplasm and facilitating a morphological study in central Andean Peru. This research was supported by the USDA and by NSF DEB 0316614 and USDA National Research Initiative Grant 2008-35300-18669 to David Spooner. NR 49 TC 14 Z9 15 U1 1 U2 3 PU INT ASSOC PLANT TAXONOMY PI VIENNA PA C/O UNIV VIENNA, INST BOTANY, RENNWEG 14, A-1030 VIENNA, AUSTRIA SN 0040-0262 J9 TAXON JI Taxon PD AUG PY 2010 VL 59 IS 4 BP 1091 EP 1101 PG 13 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 637YJ UT WOS:000280854300009 ER PT J AU Riina, R van Ee, B Wiedenhoeft, AC Cardozo, A Berry, PE AF Riina, Ricarda van Ee, Benjamin Wiedenhoeft, Alex C. Cardozo, Alfonso Berry, Paul E. TI Sectional rearrangement of arborescent clades of Croton (Euphorbiaceae) in South America: Evolution of arillate seeds and a new species, Croton domatifer SO TAXON LA English DT Article DE arillate seeds; Croton domatifer; Croton section Cuneati; Croton section Luntia; Euphorbiaceae; leaf domatia; molecular phylogeny; wood anatomy ID MOLECULAR PHYLOGENETICS; AQUEOUS EXTRACT; ESSENTIAL OIL; GENUS CROTON; CUNEATUS; MALAMBO; S.S.; INFERENCE; VENEZUELA; SYNOPSIS AB Most of the arborescent Croton species in the New World were treated by Webster as belonging either to C. sect. Cylostigma Griseb. or C. sect. Luntia (Neck. ex Raf.) G.L. Webster. The circumscription of C. sect. Cyclostigma has been treated recently. In this paper we focus on C. sect. Luntia, which was subdivided by Webster into two subsections, C. subsect. Matourenses and subsect. Cuneati. Using chloroplast trnL-F and nuclear ITS DNA sequence data, morphology and a broader sampling of additional Croton lineages, we found that the two subsections are not closely related and form distinct monophyletic groups. Substantial morphological differences support the molecular data. A taxonomic recircumscription of the two subsections, elevated to sectional level, is proposed. A key and taxonomic revision, with new synonyms, is provided for C. sect. Cuneati; together with the description of a new species from the coastal mountains of Venezuela, Croton domatifer. The new species is the only one in the genus known to possess leaf domatia. We infer that species in the Cuneati clade have lost the typical Croton caruncle, and some of them have evolved a different, specialized type of aril. We hypothesize that the arillate seeds are an adaptation to dispersal by fish in the Orinoco and Amazon river basins. C1 [Riina, Ricarda; van Ee, Benjamin; Berry, Paul E.] Univ Michigan, Herbarium, Ann Arbor, MI 48108 USA. [Riina, Ricarda; van Ee, Benjamin; Berry, Paul E.] Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48108 USA. [Riina, Ricarda] CSIC, E-28014 Madrid, Spain. [van Ee, Benjamin] Harvard Univ Hebaria, Cambridge, MA 02138 USA. [Wiedenhoeft, Alex C.] Forest Prod Lab, Madison, WI 53276 USA. [Cardozo, Alfonso] Cent Univ Venezuela, Fac Agron, Inst Bot Argricola, Maracay, Edo Aragua, Venezuela. RP Riina, R (reprint author), Univ Michigan, Herbarium, 3600 Varsity Dr, Ann Arbor, MI 48108 USA. EM riina@umich.edu RI Riina, Ricarda/J-1032-2014 OI Riina, Ricarda/0000-0002-7423-899X FU U.S. National Science Foundation [DEB-0212481]; International Association for Plant Taxonomy FX We wish to thank Bruno Manara for the illustration of C. domatifer and Kandis Elliot for the distribution map. We are grateful to the staff of A, AMAZ, BM, COAH, COL, DAV, F, G, G-DC, OH, K, LE, LPB, M, MA, MER, MICH, MO, MY, NY, S. US, USM, USZ, VEN, W, and WIS for giving us access to their collections and digital images, to Carlos Reyes, Aurimar Magallanes, and Dumas Conde for their help in the field, to Nicolas Fumeaux (G) for his invaluable help with taxonomic and nomenclatural issues, and to two anonymous reviewers for their comments, corrections, and useful suggestions. This study was supported in part by the U.S. National Science Foundation (award # DEB-0212481 to P. Berry) and by the International Association for Plant Taxonomy (award to R. Riina). NR 41 TC 17 Z9 18 U1 0 U2 4 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD AUG PY 2010 VL 59 IS 4 BP 1147 EP 1160 PG 14 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 637YJ UT WOS:000280854300014 ER PT J AU Dorr, LJ Wiersema, JH AF Dorr, Laurence J. Wiersema, John H. TI Names of American vascular plants published in Loefling's Iter Hispanicum (1758) and its German translation (1766) SO TAXON LA English DT Editorial Material DE Iter Hispanicum; Alexander Bernhard Kolpin; Carl Linnaeus; Pehr Loefling; South America; Venezuela ID THEOPHRASTACEAE; REVISION AB Loefling's Iter Hispanicum (1758) and its subsequent translations, editions, issues, and facsimiles are analyzed for their impact on the nomenclature of American vascular plants. The book, edited by Linnaeus and posthumously published, contains descriptions of plants found in Venezuela (as well as in the Iberian Peninsula). For American plants the original volume (1758) is the source of 31 new genera, 15 new species, and one replaced name, and a German translation (1766) is the source of an additional two new species. Many of these nomenclatural innovations have been ignored, overlooked, or intentionally suppressed, some for centuries. Other names in the her Hispanicum have been misinterpreted. We examine the reasons for considering these 49 names to be validly published and the nomenclatural ramifications. In the interests of nomenclatural stability we are forced to conclude that the names of at least ten taxa described by Loefling should be rejected: Ayenia sidiformis Loefl., Cofer Loefl., Cruzeta Loefl., Cruzeta hispanica Loefl., Edechia inermis Loefl., E. spinosa L. Justicia putata Loefl., Menais Loefl., Muco Loefl., and Samyda parviflora Loefl. Likewise the names of two species described by Linnaeus that are tied to the her Hispanicum should be rejected: Menais topiaria L. and Spermacoce suffruticosa L. Finally, we select neotypes for Gaura fruticosa Loefl., Salvinia michelii Loefl., and Waltheria melochioides Loefl. C1 [Dorr, Laurence J.] Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20013 USA. [Wiersema, John H.] USDA ARS, BARC W, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Dorr, LJ (reprint author), Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, MRC 166,POB 37012, Washington, DC 20013 USA. EM dorrl@si.edu NR 107 TC 3 Z9 3 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY PI VIENNA PA C/O UNIV VIENNA, INST BOTANY, RENNWEG 14, A-1030 VIENNA, AUSTRIA SN 0040-0262 J9 TAXON JI Taxon PD AUG PY 2010 VL 59 IS 4 BP 1245 EP 1262 PG 18 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 637YJ UT WOS:000280854300022 ER PT J AU Montalvo, J Von Hoven, T Cheuk, S Schindler, A AF Montalvo, J. Von Hoven, T. Cheuk, S. Schindler, A. TI Preliminary Studies of Non-Aqueous Volatiles in Lint Cotton Moisture Tests by Thermal Methods SO TEXTILE RESEARCH JOURNAL LA English DT Article DE cotton; oven-drying; non-aqueous volatiles; Karl Fischer Titration; NIR; TGA; TGA-QMS AB The standard test methods for moisture in lint cotton are based on oven drying at 105-110 degrees C. All of the loss in weight is attributable to moisture. The U. S. cotton industry questions the reliability of the oven-drying method due to the concern that non-aqueous volatiles released during drying may be of an amount sufficient to bias the results. Our approach in these initial studies was to conduct a series of probing experiments to help understand the nature of the nonaqueous volatiles. Cottons were assayed for moisture content by standard oven drying and Karl Fischer Titration. The titration results were significantly lower. We oven-dried cottons of varying mass, changed the gaseous atmosphere during heating from air to nitrogen and attempted to "fingerprint" the non-aqueous volatiles by thermogravimetric analysis and thermogravimetric analysis-quadrupole mass spectrometry. The results demonstrate that complicated side reactions occur during oven-drying. However, no specific volatile organics were detected at standard oven-drying conditions. C1 [Montalvo, J.; Von Hoven, T.; Cheuk, S.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Schindler, A.] Netzsch Geratebau GmbH, D-95100 Selb, Germany. RP Montalvo, J (reprint author), ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM joe.montalvo@ars.usda.gov FU Cotton Foundation [5001-005-00]; Cotton Incorporated [09-560]; ARS FX The authors are grateful to Jade Smith, Thomas North and Danielle Francois of SRRC for assistance in the experiments. Financial support by The Cotton Foundation project - 5001-005-00, by Cotton Incorporated - Cooperative Agreement No. 09-560, and the ARS Headquarters Funded 2008 Postdoc Program, is greatly appreciated. NR 21 TC 5 Z9 5 U1 0 U2 4 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 J9 TEXT RES J JI Text. Res. J. PD AUG PY 2010 VL 80 IS 13 BP 1360 EP 1376 DI 10.1177/0040517510365949 PG 17 WC Materials Science, Textiles SC Materials Science GA 634TV UT WOS:000280609400010 ER PT J AU Yang, XH Yan, JB Shah, T Warburton, ML Li, Q Li, L Gao, YF Chai, YC Fu, ZY Zhou, Y Xu, ST Bai, GH Meng, YJ Zheng, YP Li, JS AF Yang, Xiaohong Yan, Jianbing Shah, Trushar Warburton, Marilyn L. Li, Qing Li, Lin Gao, Yufeng Chai, Yuchao Fu, Zhiyuan Zhou, Yi Xu, Shutu Bai, Guanghong Meng, Yijiang Zheng, Yanping Li, Jiansheng TI Genetic analysis and characterization of a new maize association mapping panel for quantitative trait loci dissection SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MULTILOCUS GENOTYPE DATA; ZEA-MAYS L.; POPULATION-STRUCTURE; INBRED LINES; LINKAGE DISEQUILIBRIUM; FLOWERING-TIME; MOLECULAR MARKERS; OIL CONCENTRATION; COMPUTER-PROGRAM; GENOMIC CONTROL AB Association mapping based on the linkage disequilibrium provides a promising tool to identify genes responsible for quantitative variations underlying complex traits. Presented here is a maize association mapping panel consisting of 155 inbred lines with mainly temperate germplasm, which was phenotyped for 34 traits and genotyped using 82 SSRs and 1,536 SNPs. Abundant phenotypic and genetic diversities were observed within the panel based on the phenotypic and genotypic analysis. A model-based analysis using 82 SSRs assigned all inbred lines to two groups with eight subgroups. The relative kinship matrix was calculated using 884 SNPs with minor allele frequency a parts per thousand yen20% indicating that no or weak relationships were identified for most individual pairs. Three traits (total tocopherol content in maize kernel, plant height and kernel length) and 1,414 SNPs with missing data < 20% were used to evaluate the performance of four models for association mapping analysis. For all traits, the model controlling relative kinship (K) performed better than the model controlling population structure (Q), and similarly to the model controlling both population structure and relative kinship (Q + K) in this panel. Our results suggest this maize panel can be used for association mapping analysis targeting multiple agronomic and quality traits with optimal association model. C1 [Yang, Xiaohong; Yan, Jianbing; Li, Qing; Li, Lin; Gao, Yufeng; Chai, Yuchao; Fu, Zhiyuan; Zhou, Yi; Xu, Shutu; Bai, Guanghong; Meng, Yijiang; Zheng, Yanping; Li, Jiansheng] China Agr Univ, Natl Maize Improvement Ctr China, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China. [Yan, Jianbing; Shah, Trushar] CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico. [Warburton, Marilyn L.] ARS, USDA, Corn Host Plant Resistance Res Unit, Columbia, MS 39762 USA. [Bai, Guanghong] Xinjiang Agr Univ, Agron Coll, Urumqi 830052, Xinjiang, Peoples R China. [Shah, Trushar] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. RP Yan, JB (reprint author), China Agr Univ, Natl Maize Improvement Ctr China, Beijing Key Lab Crop Genet Improvement, Yuanmingyuan W Rd, Beijing 100193, Peoples R China. EM yjianbing@gmail.com; lijiansheng@cau.edu.cn FU National Hi-Tech Research and Development Program of China [2006AA100103, 2006AA10Z183]; National Basic Research and Development Program of China [2007CB10900] FX Helpful comments on the early manuscript from Drs. Michael Gore and Maruthi Prasanna Boddupalli are appreciated. The authors gratefully thank the editor Dr. Jianming Yu and three anonymous reviewers for their valuable suggestions. This research was supported by National Hi-Tech Research and Development Program of China (2006AA100103, 2006AA10Z183), National Basic Research and Development Program of China (2007CB10900). NR 86 TC 74 Z9 81 U1 4 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 3 BP 417 EP 431 DI 10.1007/s00122-010-1320-y PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 628BZ UT WOS:000280090700002 PM 20349034 ER PT J AU Bento, M Gustafson, P Viegas, W Silva, M AF Bento, Miguel Gustafson, Perry Viegas, Wanda Silva, Manuela TI Genome merger: from sequence rearrangements in triticale to their elimination in wheat-rye addition lines SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID NUCLEOLAR DOMINANCE; GENE-EXPRESSION; DNA-SEQUENCES; ALLOPOLYPLOIDIZATION; EVOLUTION; DYNAMICS; POLYPLOIDIZATION; HETEROCHROMATIN; HYBRIDIZATION; METHYLATION AB Genetic and epigenetic modifications resulting from different genomes adjusting to a common nuclear environment have been observed in polyploids. Sequence restructuring within genomes involving retrotransposon/microsatellite-rich regions has been reported in triticale. The present study uses inter-retrotransposon amplified polymorphisms (IRAP) and retrotransposon microsatellite amplified polymorphisms (REMAP) to assess genome rearrangements in wheat-rye addition lines obtained by the controlled backcrossing of octoploid triticale to hexaploid wheat followed by self-fertilization. The comparative analysis of IRAP and REMAP banding profiles, involving a complete set of wheat-rye addition lines, and their parental species revealed in those lines the presence of wheat-origin bands absent in triticale, and the absence of rye-origin and triticale-specific bands. The presence in triticale x wheat backcrosses (BC) of rye-origin bands that were absent in the addition lines demonstrated that genomic rearrangement events were not a direct consequence of backcrossing, but resulted from further genome structural rearrangements in the BC plant progeny. PCR experiments using primers designed from different rye-origin sequences showed that the absence of a rye-origin band in wheat-rye addition lines results from sequence elimination rather than restrict changes on primer annealing sites, as noted in triticale. The level of genome restructuring events evaluated in all seven wheat-rye addition lines, compared to triticale, indicated that the unbalanced genome merger situation observed in the addition lines induced a new round of genome rearrangement, suggesting that the lesser the amount of rye chromatin introgressed into wheat the larger the outcome of genome reshuffling. C1 [Bento, Miguel; Viegas, Wanda; Silva, Manuela] Univ Tecn Lisbon, Ctr Bot Aplicada Agr, Seccao Genet, Inst Super Agron, P-1349017 Lisbon, Portugal. [Gustafson, Perry] Univ Missouri, USDA ARS, PGRU, Columbia, MO 65211 USA. RP Silva, M (reprint author), Univ Tecn Lisbon, Ctr Bot Aplicada Agr, Seccao Genet, Inst Super Agron, P-1349017 Lisbon, Portugal. EM manuelasilva@isa.utl.pt OI Bento, Miguel/0000-0002-7545-8943; Viegas, Wanda/0000-0003-4660-1226; Gomes da Silva, Manuela/0000-0003-4677-870X FU Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/28657/2006]; Fundacao para a Ciencia e a Tecnologia [PPCDT/BIABDE/57575/2004, PTDC/BIA-BEC/101964/2008] FX Miguel Bento is funded by a doctoral scholarship (SFRH/BD/28657/2006) by Fundacao para a Ciencia e a Tecnologia, Portugal. This research was financed by Fundacao para a Ciencia e a Tecnologia (Projects PPCDT/BIABDE/57575/2004 and PTDC/BIA-BEC/101964/2008). We would like to thank Augusta Barao for her excellent technical assistance and Leonor Morais-Cecilio for her help in image processing. NR 31 TC 24 Z9 32 U1 0 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 3 BP 489 EP 497 DI 10.1007/s00122-010-1325-6 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 628BZ UT WOS:000280090700007 PM 20383487 ER PT J AU Harel-Beja, R Tzuri, G Portnoy, V Lotan-Pompan, M Lev, S Cohen, S Dai, N Yeselson, L Meir, A Libhaber, SE Avisar, E Melame, T van Koert, P Verbakel, H Hofstede, R Volpin, H Oliver, M Fougedoire, A Stalh, C Fauve, J Copes, B Fei, Z Giovannoni, J Ori, N Lewinsohn, E Sherman, A Burger, J Tadmor, Y Schaffer, AA Katzir, N AF Harel-Beja, R. Tzuri, G. Portnoy, V. Lotan-Pompan, M. Lev, S. Cohen, S. Dai, N. Yeselson, L. Meir, A. Libhaber, S. E. Avisar, E. Melame, T. van Koert, P. Verbakel, H. Hofstede, R. Volpin, H. Oliver, M. Fougedoire, A. Stalh, C. Fauve, J. Copes, B. Fei, Z. Giovannoni, J. Ori, N. Lewinsohn, E. Sherman, A. Burger, J. Tadmor, Y. Schaffer, A. A. Katzir, N. TI A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; ALKALINE ALPHA-GALACTOSIDASE; SUCROSE-PHOSPHATE SYNTHASE; RECOMBINANT INBRED LINES; NEAR-ISOGENIC LINES; CUCUMIS-MELO; TOMATO FRUIT; ACID INVERTASE; BETA-CAROTENE; FUSARIUM-WILT AB A genetic map of melon enriched for fruit traits was constructed, using a recombinant inbred (RI) population developed from a cross between representatives of the two subspecies of Cucumis melo L.: PI 414723 (subspecies agrestis) and 'Dulce' (subspecies melo). Phenotyping of 99 RI lines was conducted over three seasons in two locations in Israel and the US. The map includes 668 DNA markers (386 SSRs, 76 SNPs, six INDELs and 200 AFLPs), of which 160 were newly developed from fruit ESTs. These ESTs include candidate genes encoding for enzymes of sugar and carotenoid metabolic pathways that were cloned from melon cDNA or identified through mining of the International Cucurbit Genomics Initiative database (http://www.icugi.org/). The map covers 1,222 cM with an average of 2.672 cM between markers. In addition, a skeleton physical map was initiated and 29 melon BACs harboring fruit ESTs were localized to the 12 linkage groups of the map. Altogether, 44 fruit QTLs were identified: 25 confirming QTLs described using other populations and 19 newly described QTLs. The map includes QTLs for fruit sugar content, particularly sucrose, the major sugar affecting sweetness in melon fruit. Six QTLs interacting in an additive manner account for nearly all the difference in sugar content between the two genotypes. Three QTLs for fruit flesh color and carotenoid content were identified. Interestingly, no clear colocalization of QTLs for either sugar or carotenoid content was observed with over 40 genes encoding for enzymes involved in their metabolism. The RI population described here provides a useful resource for further genomics and metabolomics studies in melon, as well as useful markers for breeding for fruit quality. C1 [Harel-Beja, R.; Tzuri, G.; Portnoy, V.; Lotan-Pompan, M.; Lev, S.; Meir, A.; Libhaber, S. E.; Lewinsohn, E.; Burger, J.; Tadmor, Y.; Katzir, N.] Newe Yaar Res Ctr, Agr Res Org, Dept Vegetable Res, IL-30095 Ramat Yishay, Israel. [Cohen, S.; Dai, N.; Yeselson, L.; Avisar, E.; Melame, T.; Sherman, A.; Schaffer, A. A.] Agr Res Org, Dept Vegetable Res, Volcani Res Ctr, IL-50250 Bet Dagan, Israel. [van Koert, P.; Verbakel, H.; Hofstede, R.; Volpin, H.] Keygene NV, NL-6708 Wageningen, Netherlands. [Oliver, M.] Syngenta Seeds SAS, F-31790 St Sauveur, France. [Fougedoire, A.; Stalh, C.; Copes, B.] HM Clause, Davis, CA 95618 USA. [Fauve, J.] Clause, F-13210 St Remy De Provence, France. [Ori, N.] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Fac Agr Food & Environm, IL-76100 Rehovot, Israel. [Fei, Z.; Giovannoni, J.] Cornell Univ, USDA, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Katzir, N (reprint author), Newe Yaar Res Ctr, Agr Res Org, Dept Vegetable Res, POB 1021, IL-30095 Ramat Yishay, Israel. EM katzirn@agri.gov.il FU BARD [IS-3877-06]; United States-Israel Binational Agricultural Research and Development Fund; Israel Science Foundation [386-06] FX We are deeply grateful to the staff of the Cancer Research Center and Paediatric Haematology-Oncology at Sheba Medical Center for the Sequenom analysis. We are most thankful to Dr. Ilan Paran for fruitful discussions throughout the project, to Dr. Harry Paris for critically reviewing the manuscript and to Uzi Sa'ar and Fabian Baumkoler for assistance with the Weld management. We thank the Galilee Technology Center, Migal for the DHPLC analysis. This research was supported by Research Grant Award No. IS-3877-06 from BARD, the United States-Israel Binational Agricultural Research and Development Fund, and in part by Israel Science Foundation Grant No. 386-06. The research was also supported by De Ruiter Seeds, Enza Zaden, Rijk Zwaan, Sakata Seed Corporation, Semilas Fito, Syngenta Seeds and Vilmorin Clause and Cie. Contribution No. 109/2010 of the Agricultural Research Organization, Bet Dagan Israel. NR 75 TC 52 Z9 63 U1 7 U2 46 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 3 BP 511 EP 533 DI 10.1007/s00122-010-1327-4 PG 23 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 628BZ UT WOS:000280090700009 PM 20401460 ER PT J AU Kim, KS Hill, CB Hartman, GL Hyten, DL Hudson, ME Diers, BW AF Kim, Ki-Seung Hill, Curtis B. Hartman, Glen L. Hyten, David L. Hudson, Matthew E. Diers, Brian W. TI Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID SINGLE DOMINANT GENE; ROOT-KNOT NEMATODE; DISEASE; LOCUS; RECOMBINATION; INHERITANCE; EXPRESSION; PI-243540; HOMOPTERA; FAMILIES AB The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from 'Dowling'. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping was carried out using lines derived from 5,783 F(2) plants at different levels of backcrossing that were screened with flanking genetic markers for the presence of recombination in the Rag2 interval. Fifteen single nucleotide polymorphism (SNP) markers and two dominant polymerase chain reaction-based markers near Rag2 were developed by re-sequencing target intervals and sequence-tagged sites. These efforts resulted in the mapping of Rag2 to a 54-kb interval on the Williams 82 8x assembly (Glyma1). This Williams 82 interval contains seven predicted genes, which includes one nucleotide-binding site-leucine-rich repeat gene. SNP marker and candidate gene information identified in this study will be an important resource in marker-assisted selection for aphid resistance and for cloning the gene. C1 [Kim, Ki-Seung; Hill, Curtis B.; Hartman, Glen L.; Hudson, Matthew E.; Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Hyten, David L.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Diers, BW (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM bdiers@illinois.edu RI Hudson, Matthew/A-4438-2008; OI Hudson, Matthew/0000-0002-4737-0936; Hyten, David/0000-0001-6324-9389 FU Illinois Soybean Association; United Soybean Board; USDA CSREES [AG2006-34488-16915] FX This work was supported by soybean check-off funding from the Illinois Soybean Association, the United Soybean Board, and the USDA CSREES grant #AG2006-34488-16915 to MEH and BD. NR 43 TC 28 Z9 31 U1 1 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 3 BP 599 EP 610 DI 10.1007/s00122-010-1333-6 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 628BZ UT WOS:000280090700015 PM 20454773 ER PT J AU Tsilo, TJ Hareland, GA Simsek, S Chao, SM Anderson, JA AF Tsilo, Toi J. Hareland, Gary A. Simsek, Senay Chao, Shiaoman Anderson, James A. TI Genome mapping of kernel characteristics in hard red spring wheat breeding lines SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GRAIN PROTEIN-CONTENT; TRITICUM-AESTIVUM L; BREAD-WHEAT; WINTER-WHEAT; MICROSATELLITE MARKERS; GENETIC-ANALYSIS; SSR MARKERS; YIELD; SELECTION; QUALITY AB Kernel characteristics, particularly kernel weight, kernel size, and grain protein content, are important components of grain yield and quality in wheat. Development of high performing wheat cultivars, with high grain yield and quality, is a major focus in wheat breeding programs worldwide. Here, we report chromosome regions harboring genes that influence kernel weight, kernel diameter, kernel size distribution, grain protein content, and grain yield in hard red spring wheat breeding lines adapted to the Upper Midwest region of the United States. A genetic linkage map composed of 531 SSR and DArT marker loci spanned a distance of 2,505 cM, covering all 21 chromosomes of wheat. Stable QTL clusters influencing kernel weight, kernel diameter, and kernel size distribution were identified on chromosomes 2A, 5B, and 7A. Phenotypic variation explained by individual QTL at these clusters varied from 5 to 20% depending on the trait. A QTL region on chromosome 2B confers an undesirable pleiotropic effect or a repulsion linkage between grain yield (LOD = 6.7; R (2) = 18%) and grain protein content (LOD = 6.2; R (2) = 13.3%). However, several grain protein and grain yield QTL independent of each other were also identified. Because some of the QTL identified in this study were consistent across environments, DNA markers will provide an opportunity for increasing the frequency of desirable alleles through marker-assisted selection. C1 [Tsilo, Toi J.; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Hareland, Gary A.] ARS, Wheat Qual Lab, USDA, Fargo, ND 58105 USA. [Simsek, Senay] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Chao, Shiaoman] ARS, Biosci Res Lab, USDA, Fargo, ND 58105 USA. RP Tsilo, TJ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall, St Paul, MN 55108 USA. EM tsilo001@umn.edu; ander319@umn.edu RI Tsilo, Toi/G-5088-2011 FU Minnesota Annual Conference of the United Methodist Church; Compton International fellowship; National Research Foundation of South Africa; Department of Science and Technology of South Africa; Agricultural Research Council of South Africa; United State Department of Agriculture-Agricultural Research Service; USDA Cooperative Research, Education and Extension Service [2006-55606-16629] FX The authors gratefully acknowledge financial support from the Minnesota Annual Conference of the United Methodist Church, the Compton International fellowship, National Research Foundation of South Africa, Department of Science and Technology of South Africa, Agricultural Research Council of South Africa, the United State Department of Agriculture-Agricultural Research Service, and the USDA Cooperative Research, Education and Extension Service, Coordinated Agricultural Project grant number 2006-55606-16629. The authors would like to thank the University of Minnesota wheat breeding scientists, Gary Linkert and Catherine Springer, and the USDA/ARS personnel, Dale Hanson, Dehdra Puhr, and Jadene Wear, for their technical assistance. NR 53 TC 58 Z9 63 U1 0 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 4 BP 717 EP 730 DI 10.1007/s00122-010-1343-4 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630LY UT WOS:000280276000010 PM 20425103 ER PT J AU Riday, H Krohn, AL AF Riday, Heathcliffe Krohn, Andrew L. TI Genetic map-based location of the red clover (Trifolium pratense L.) gametophytic self-incompatibility locus SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID S-RNASE; POPULATIONS; ALLELES; LINKAGE; PLANTS AB Red clover is a hermaphroditic allogamous diploid (2n = 2x = 14) with a homomorphic gametophytic self-incompatibility (GSI) system (Trifolium pratense L.). Red clover GSI has long been studied, and it is thought that the genetic control of GSI constitutes a single locus. Although GSI genes have been identified in other species, the genomic location of the red clover GSI-locus remains unknown. The objective of this study was to use a mapping-based approach to identify simple sequence repeats (SSR) that were closely linked to the GSI-locus. Previously published SSR markers were used in this effort (Sato et al. in DNA Res 12:301-364, 2005). A bi-parental cross was initiated in which the parents were known to have one self-incompatibility allele (S-allele) in common. S-allele genotypes of 100 progeny were determined through test crosses and pollen compatibility. Pseudo F(1) linkage analysis isolated the GSI-locus on red clover linkage-group one within 2.5 cM of markers RCS5615, RCS0810, and RCS3161. A second 256 progeny mapping testcross population of a heterozygous self-compatible mutant revealed that this specific self-compatible mutant mapped to the same location as the GSI-locus. Finally, 82 genotypes were identified whose parents putatively shared one S-allele in common from maternal halfsib families derived from two random mating populations in which paternal identity was determined using molecular markers. Unique S-allele identity in the two random mating populations was tentatively inferred based on haplotypes of two highly allelic linkage-group one SSR (RCS0810 and RCS4956), which were closely linked to each other and the GSI-locus. Paternally derived pollen haplotype linkage analysis of RCS0810 and RCS4956 SSR and the GSI-locus again revealed tight linkage at 2.5 and 4.7 cM between the GSI-locus and RCS0810 and RCS4956, respectively. The map-based location of the GSI-locus in red clover has many immediate applications to red clover plant breeding and could be useful in helping to sequence the GSI-locus. C1 [Riday, Heathcliffe] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Krohn, Andrew L.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. RP Riday, H (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM heathcliffe.riday@ars.usda.gov NR 29 TC 4 Z9 5 U1 2 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2010 VL 121 IS 4 BP 761 EP 767 DI 10.1007/s00122-010-1347-0 PG 7 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 630LY UT WOS:000280276000014 PM 20461353 ER PT J AU Henry, WB Krakowsky, MD Windham, GL Williams, WP Scully, BT Rowe, D Hawkins, LK AF Henry, W. Brien Krakowsky, Matt D. Windham, Gary L. Williams, W. Paul Scully, Brian T. Rowe, Dennis Hawkins, Leigh K. TI Comparison of the side-needle and knife techniques for inducing Aspergillus flavus infection and aflatoxin accumulation in corn hybrids SO TOXIN REVIEWS LA English DT Article DE Aspergillus flavus; inoculation; maize; aflatoxin; side-needle ID INOCULATION TECHNIQUES; HEPATOCELLULAR-CARCINOMA; KERNEL INFECTION; MAIZE; CONTAMINATION; FIELD; RESISTANCE; GERMPLASM; GENOTYPES; INBREDS AB Evaluation of corn genotypes for resistance to aflatoxin accumulation has evolved over the past 30 years. Inoculation techniques have been developed to ensure that plants are exposed to Aspergillus flavus and resistant genotypes can be identified. We compared two inoculation techniques (side needle and knife) and different inoculation rates in Georgia and Mississippi. The relative performance of side-needle and knife inoculation techniques did not change or interact significantly across site years, suggesting that both the needle and knife are consistent inoculation techniques for discriminating between resistant and susceptible corn hybrids at the Mississippi State and Tifton locations. C1 [Henry, W. Brien] ARS, Corn Host Plant Resistance Res Unit, USDA, Mississippi State, MS 39762 USA. [Krakowsky, Matt D.] ARS, Plant Sci Res Unit, Raleigh, NC USA. [Scully, Brian T.] ARS, Crop Protect & Management Res Unit, Tifton, GA USA. [Rowe, Dennis] Mississippi State Univ, Mississippi State, MS 39762 USA. RP Henry, WB (reprint author), ARS, Corn Host Plant Resistance Res Unit, USDA, POB 5367, Mississippi State, MS 39762 USA. EM Brien.Henry@ars.usda.gov NR 34 TC 4 Z9 4 U1 0 U2 2 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 1556-9543 J9 TOXIN REV JI Toxin Rev. PD AUG PY 2010 VL 29 IS 3-4 BP 123 EP 129 DI 10.3109/15569543.2010.517616 PG 7 WC Toxicology SC Toxicology GA 659SZ UT WOS:000282588500005 ER PT J AU Duke, SO Cantrell, CL Meepagala, KM Wedge, DE Tabanca, N Schrader, KK AF Duke, Stephen O. Cantrell, Charles L. Meepagala, Kumudini M. Wedge, David E. Tabanca, Nurhayat Schrader, Kevin K. TI Natural Toxins for Use in Pest Management SO TOXINS LA English DT Review DE algicide; fungicide; herbicide; insecticide; molluscicide; pesticide AB Natural toxins are a source of new chemical classes of pesticides, as well as environmentally and toxicologically safer molecules than many of the currently used pesticides. Furthermore, they often have molecular target sites that are not exploited by currently marketed pesticides. There are highly successful products based on natural compounds in the major pesticide classes. These include the herbicide glufosinate (synthetic phosphinothricin), the spinosad insecticides, and the strobilurin fungicides. These and other examples of currently marketed natural product-based pesticides, as well as natural toxins that show promise as pesticides from our own research are discussed. C1 [Duke, Stephen O.; Cantrell, Charles L.; Meepagala, Kumudini M.; Wedge, David E.; Tabanca, Nurhayat; Schrader, Kevin K.] ARS, USDA, University, MS 38677 USA. RP Duke, SO (reprint author), ARS, USDA, POB 8048, University, MS 38677 USA. EM Stephen.duke@ars.usda.gov; charles.cantrell@ars.usda.gov; Kumudini.Meepagala@ars.usda.gov; david.wedge@ars.usda.gov; Nur.tabanca@ars.usda.gov; Kevin.schrader@ars.usda.gov NR 78 TC 37 Z9 42 U1 3 U2 38 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD AUG PY 2010 VL 2 IS 8 BP 1943 EP 1962 DI 10.3390/toxins2081943 PG 20 WC Toxicology SC Toxicology GA V24UN UT WOS:000208435400002 PM 22069667 ER PT J AU Schiller, I Oesch, B Vordermeier, HM Palmer, MV Harris, BN Orloski, KA Buddle, BM Thacker, TC Lyashchenko, KP Waters, WR AF Schiller, I. Oesch, B. Vordermeier, H. M. Palmer, M. V. Harris, B. N. Orloski, K. A. Buddle, B. M. Thacker, T. C. Lyashchenko, K. P. Waters, W. R. TI Bovine Tuberculosis: A Review of Current and Emerging Diagnostic Techniques in View of their Relevance for Disease Control and Eradication SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Review DE tuberculosis; bovine; eradication; Mycobacterium bovis; cell-mediated immunity test; serology; culture; PCR; strain typing ID GAMMA-INTERFERON ASSAY; AVIUM SUBSP-PARATUBERCULOSIS; EXPERIMENTALLY INFECTED CATTLE; ENZYME-LINKED-IMMUNOSORBENT; HUMORAL IMMUNE-RESPONSES; MYCOBACTERIUM-BOVIS; ANTIBODY-RESPONSES; GENE-EXPRESSION; WHOLE-BLOOD; HYPERSENSITIVITY RESPONSES AB P>Existing strategies for long-term bovine tuberculosis (bTB) control/eradication campaigns are being reconsidered in many countries because of the development of new testing technologies, increased global trade, continued struggle with wildlife reservoirs of bTB, redistribution of international trading partners/agreements, and emerging financial and animal welfare constraints on herd depopulation. Changes under consideration or newly implemented include additional control measures to limit risks with imported animals, enhanced programs to mitigate wildlife reservoir risks, re-evaluation of options to manage bTB-affected herds/regions, modernization of regulatory framework(s) to re-focus control efforts, and consideration of emerging testing technologies (i.e. improved or new tests) for use in bTB control/eradication programs. Traditional slaughter surveillance and test/removal strategies will likely be augmented by incorporation of new technologies and more targeted control efforts. The present review provides an overview of current and emerging bTB testing strategies/tools and a vision for incorporation of emerging technologies into the current control/eradication programs. C1 [Schiller, I.] Fed Vet Off, CH-3003 Bern, Switzerland. [Oesch, B.] Malcisbo AG, Zurich, Switzerland. [Vordermeier, H. M.] Vet Lab Agcy, Addlestone, Surrey, England. [Palmer, M. V.; Thacker, T. C.; Waters, W. R.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Harris, B. N.] USDA, Natl Vet Serv Labs, Mycobacteria & Brucella Sect, Ames, IA 50010 USA. [Orloski, K. A.] Vet Serv, USDA, Ft Collins, CO USA. [Buddle, B. M.] AgRes, Palmerston North, New Zealand. [Lyashchenko, K. P.] Chembio Diagnost Syst Inc, Medford, NY USA. RP Schiller, I (reprint author), Fed Vet Off, Schwarzenburgstr 155, CH-3003 Bern, Switzerland. EM irene.schiller@bvet.admin.ch RI Vordermeier, H Martin/C-6936-2011; APHA, Staff publications/E-6082-2010; OI Thacker, Tyler/0000-0001-6779-7649 NR 109 TC 89 Z9 90 U1 4 U2 34 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1865-1674 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD AUG PY 2010 VL 57 IS 4 BP 205 EP 220 DI 10.1111/j.1865-1682.2010.01148.x PG 16 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA 621XS UT WOS:000279620600001 PM 20561288 ER PT J AU Kelleners, TJ Chandler, DG McNamara, JP Gribb, MM Seyfried, MS AF Kelleners, T. J. Chandler, D. G. McNamara, J. P. Gribb, M. M. Seyfried, M. S. TI Modeling Runoff Generation on in a Small Snow-Dominated Mountainous Catchment SO VADOSE ZONE JOURNAL LA English DT Article ID SOIL-WATER; CONSERVATION EQUATIONS; STREAMFLOW GENERATION; COMPLEX TERRAIN; ENERGY-BALANCE; HYDROLOGY; OPTIMIZATION; SIMULATIONS; AREAS; FLOW AB Snowmelt in mountainous areas is an important contributor to river water flows in the western United States. We developed a distributed model that calculates solar radiation on, canopy energy balance, surface energy balance, snow pack dynamics, soil water flow, snow-soil-bedrock heat exchange, soil water freezing, and lateral surface and subsurface water flow. The model was applied to describe runoff generation on in a subcatchment of the Dry Creek Experimental Watershed near Boise, ID. Calibration on was achieved by optimizing the soil water field capacity (a trigger for lateral subsurface flow), lateral saturated soil hydraulic conductivity, and vertical saturated hydraulic conductivity of the bedrock. Validation on results show that the model can successfully calculate snow dynamics, soil water content, and soil temperature. Modeled streamflow for the validaion period was underestimated by 53%. The timing of the streamflow was captured reasonably well (modeling efficiency was 0.48 for the validation period). The model calculations suggest that 50 to 53% of the yearly incoming precipitation in the subcatchment is consumed by evapotranspiration. The model results further suggest that 34 to 36% of the incoming precipitation is transformed into deep percolation into the bedrock, while only 11 to 16% is transformed into streamflow. C1 [Kelleners, T. J.] Univ Wyoming, Renewable Resources Dep, Laramie, WY 82071 USA. [Chandler, D. G.] Kansas State Univ, Dep Civil Engn, Manhattan, KS 66506 USA. [McNamara, J. P.] Boise State Univ, Dep Geosci, Boise, ID 83725 USA. [Gribb, M. M.] Boise State Univ, Dep Civil Engn, Boise, ID 83725 USA. [Seyfried, M. S.] USDA ARS, NW Watershed Res Ctr, Boise, ID 83712 USA. RP Kelleners, TJ (reprint author), Univ Wyoming, Renewable Resources Dep, Laramie, WY 82071 USA. EM tkellene@uwyo.edu RI McNamara, James/F-1993-2011; Chandler, David/E-4543-2013 OI Chandler, David/0000-0002-8662-2892 FU NSF [EPS-0447689]; NASA [NAG5-7537]; USDA-NRI [2001-35102-11031]; USDA-CSREES SRGP [2005-34552-15828] FX The Dry Creek Experimental Watershed is jointly operated by Boise State University and the USDA Northwest Watershed Research Center. Instrumentation was supported by NSF Grant EPS-0447689, NASA Grant NAG5-7537, and USDA-NRI Grant 2001-35102-11031. This study was funded in part by USDA-CSREES SRGP Award 2005-34552-15828. NR 48 TC 12 Z9 12 U1 1 U2 16 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2010 VL 9 IS 3 BP 517 EP 527 DI 10.2136/vzj2009.0033 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 678FE UT WOS:000284058700002 ER PT J AU Ryel, RJ Leffler, AJ Ivans, C Peek, MS Caldwell, MM AF Ryel, Ronald J. Leffler, A. Joshua Ivans, Carolyn Peek, Michael S. Caldwell, Martyn M. TI Functional Differences in Water-Use Patterns of Contrasting Life Forms in Great Basin Steppelands SO VADOSE ZONE JOURNAL LA English DT Article ID PINYON-JUNIPER WOODLAND; BENEATH DESERT SOILS; CHANGE-TYPE DROUGHT; ARTEMISIA-TRIDENTATA; HYDRAULIC REDISTRIBUTION; CHEATGRASS COMMUNITY; SUMMER PRECIPITATION; SEMIARID LANDSCAPES; ECOSYSTEM FUNCTION; CHIHUAHUAN DESERT AB The temporal patterns of evapotranspiration were monitored for 2 yr for four species of differing life form that currently form near monoculture communities in the Great Basin, USA, a region with a growing season spanning early spring to autumn and predictable overwinter water accumulation in the vadose zone. Species included an annual grass (Bromus tectorum L.), a perennial grass [Agropyron desertorum (Fisch. ex Link) Schult.], a shrub (Artemisia tridentata Nutt. ssp. wyomingensis Beetle and Young), and a tree [Juniperus osteosperma (Torr.) Little]. The two grasses and shrub were growing on the same soil type with uniform texture and subject to near surface percolation of the vadose zone only, while J. osteosperma was growing on soils with a petrocalcic layer below which water was near field capacity. These monotypic stands were found to differ in quantity and timing of vadose zone water use, in use pattern of shallow and deeper water resource pools, and in depth and quantity of rainwater hydraulically redistributed. All species rapidly utilized shallow vadose zone water in the spring when growth was observed, but use of deep vadose zone water varied by life form and was not linked with the period of growth for any species. Water in the vadose zone of the grass species increased between years, with evapotranspiration less than precipitation inputs and contrasted to water use in A. tridentata where water use approximately equaled precipitation inputs. Juniperus osteosperma used water below the petrocalcic zone, particularly in late summer. Water use by all species was consistent with the concept of a shallow vadose zone "growth pool" of water and a deeper vadose zone "maintenance pool" used during the summer drought period. The patterns of water use suggest that water per se is not a limited resource for survival, but influences the availability of nutrients necessary for plant growth that are associated with shallow vadose zone water. We postulate that cold-adapted plants in the Great Basin have converged on a general pattern of rapidly utilizing soil moisture in shallow depths, in part, to influence nutrient availability. Our results strongly suggest describing the pool dynamics of vadose zone water will be necessary to further our understanding of plant fitness, interactions among species for resources, and species coexistence in arid and semiarid ecosystems. C1 [Ryel, Ronald J.; Caldwell, Martyn M.] Utah State Univ, Dep Wildland Resources, Logan, UT 84322 USA. [Ryel, Ronald J.; Caldwell, Martyn M.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Leffler, A. Joshua] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Ivans, Carolyn] Campbell Sci Inc, Logan, UT 84321 USA. [Peek, Michael S.] William Paterson Univ, Dep Biol, Wayne, NJ 07470 USA. RP Ryel, RJ (reprint author), Utah State Univ, Dep Wildland Resources, Logan, UT 84322 USA. EM ron.ryel@usu.edu RI Ryel, Ronald/A-3422-2008 FU National Science Foundation [DEB-9807097]; Utah Agricultural Experiment Station FX We thank Ann Mull for excellent field assistance, Charles Ashurst for superb instrumentation expertise, and Darrell Johnson for being very gracious to allow us to establish our study site on his property in Rush Valley, Utah. This work was funded by the National Science Foundation (DEB-9807097) and the Utah Agricultural Experiment Station. NR 114 TC 30 Z9 31 U1 6 U2 43 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2010 VL 9 IS 3 BP 548 EP 560 DI 10.2136/vzj2010.0022 PG 13 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 678FE UT WOS:000284058700005 ER PT J AU Estrada-Medina, H Tuttle, W Graham, RC Allen, MF Jimenez-Osornio, JJ AF Estrada-Medina, Hector Tuttle, Wes Graham, Robert C. Allen, Michael F. Jose Jimenez-Osornio, Juan TI Identification of Underground Karst Features using Ground-Penetrating Radar in Northern Yucatan, Mexico SO VADOSE ZONE JOURNAL LA English DT Article ID DRY TROPICAL CLIMATE; WATER; GPR; SOILS; LIMESTONE; FLORIDA; AREAS AB Northern Yucatan is a dry tropical area where limestone karst terrain supports a subdeciduous forest that is critical for sustaining the local economy of Mayan people. The 5- to 10-m-deep vadose zone is characterized by shallow soils (< 30 cm thick) with frequent rock outcrops overlying limestone bedrock, which contains the aquifer. This limestone has two important characteristics: (i) lithologic properties that change with depth and (ii) numerous dissolution cavities ranging from small pores to caves, some of them filled with soil (soil pockets). Due to soil shallowness, differences in rock properties are relevant because they restrict or favor root growth; in particular, caves, crevices, and soil pockets allow preferential movement of soil, water, and roots. These features may be important for water and nutrient availability, especially for tree species, but they have not been investigated within an ecological context. We studied the capability of ground-penetrating radar (GPR) for identifying various limestone layers-the laja (0.3-2.5 m), the sascab (2.5-5 m), and the coquina (5-9 m)-and dissolution cavities. Research was conducted in a limestone quarry so that radar records could be compared with karst features revealed as blasting and rock extraction advanced. Radar records obtained using a 200-MHz antenna were generally of good interpretative quality; observation depths ranged from 3 to 5 m. In areas that lacked a soil mantle, the interface between the laja and the sascab was clearly identified by GPR at about 2 m. However, the sascab-coquina, and coquina-aquifer boundaries could not be identified. Areas of deep soils (> 1 m), microrelief (mounds and plains), and large soil pockets within the limestone matrix were also identified. Main sources of GPR signal attenuation were suspected to be the higher clay and water contents of soil material contained in underlying cavities. As a noninvasive tool, GPR can help to determine properties of the limestone and its dissolution features that are critical to vadose zone-forest interactions. C1 [Estrada-Medina, Hector; Graham, Robert C.] Univ Calif Riverside, Soil & Water Sci Program, Riverside, CA 92521 USA. [Estrada-Medina, Hector; Jose Jimenez-Osornio, Juan] Univ Autonoma Yucatan, Dept Manejo & Conservac Recursos Nat Trop PROTROP, Merida 97315, Yucatan, Mexico. [Tuttle, Wes] USDA NRCS, Natl Soil Survey Ctr, Lincoln, NE 68508 USA. [Allen, Michael F.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. RP Estrada-Medina, H (reprint author), Univ Calif Riverside, Soil & Water Sci Program, Riverside, CA 92521 USA. EM hector.estrada@uady.mx RI Estrada Medina, Hector/A-7665-2014 FU CONACyT-UCMEXUS; UCMEXUS; National Science Foundation [DEB 0615427, EF0410408, CRR-0120778]; UCR Center for Conservation Biology FX We thank CONACyT-UCMEXUS for the scholarship granted to the first author for doctoral studies at the University of California Riverside. This work was supported by UCMEXUS through the projects: "Importance of limestone bedrock and subsurface pockets of soil as potential sources of water for dry deciduous tree species in northern Yucatan" and "Water contribution by limestone bedrock and subsurface soil pockets and its effect on the water use efficiency of two tree species in Yucatan, Mexico"; grants from the National Science Foundation (DEB 0615427, EF0410408, and CRR-0120778), and the UCR Center for Conservation Biology. We would like to thank the owners of the quarry, especially Hector Buenfil Cervera; the workers of the quarry, especially Roger Armando Fernandez Paredes, and Augusto Cervera Buenfil for helping with the work logistics; Alfonso Castillo, Mar a Jose Romero, Raul Hernandez, and Ariel Esparza for helping with the field and laboratory work; David R. Weise from the Forest Fire Laboratory (USFS) at Riverside who kindly lent us a GPR antenna for our preliminary probes; Dr. James Doolittle and Tricia Menchaca for their constructive comments on the manuscript. NR 48 TC 7 Z9 7 U1 2 U2 30 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2010 VL 9 IS 3 BP 653 EP 661 DI 10.2136/vzj2009.0116 PG 9 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 678FE UT WOS:000284058700014 ER PT J AU Yakirevich, A Gish, TJ Simunek, J van Genuchten, MT Pachepsky, YA Nicholson, TJ Cady, RE AF Yakirevich, A. Gish, T. J. Simunek, J. van Genuchten, M. Th Pachepsky, Y. A. Nicholson, T. J. Cady, R. E. TI Potential Impact of a Seepage Face on Solute Transport to a Pumping Well SO VADOSE ZONE JOURNAL LA English DT Article ID UNSATURATED POROUS-MEDIA; TILE-DRAINED FIELD; HYDRAULIC CONDUCTIVITY; PREFERENTIAL FLOW; BROMIDE TRANSPORT; GROUNDWATER-FLOW; CAPILLARY-FRINGE; WATER SATURATION; STEADY-STATE; SOIL AB To develop predictive models for solute transport in the subsurface, the mechanisms governing transport must be understood and quantified. In this study, we used the HYDRUS-2D variably saturated flow and transport model to describe the observed mass flux breakthrough curves (BTCs) of three surface-applied tracers-Br-, Cl-, and pentafluorobenzoic acid (PFBA)-to a single well from which the tracers were pumped. Axisymmetrical transport simulations of the data indicated the presence of an active seepage face along the soil-well interface near the water table. The calculated cumulative water flux to the well through the seepage face was found to be 120% of the variable flux boundary into the well through the submerged zone. In addition, calculated mass fluxes of Br, Cl, and PFBA through the seepage interface were approximately 8, 4, and 11 times, respectively, those through the variable flux boundary. Calculations suggest that a seepage face may be responsible for causing the early arrival of the solutes and the overall shape of the BTCs. Our study indicates the potentially important role a seepage face along the soil-well interface may have in modeling water flow and chemical transport to wells in variably saturated, unconfined aquifers. C1 [Gish, T. J.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Yakirevich, A.] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Zuckerberg Inst Water Sci & Technol, IL-84990 Sede Boqer, Israel. [Simunek, J.] Univ Calif Riverside, Dep Environm Sci, Riverside, CA 92521 USA. [van Genuchten, M. Th] Univ Fed Rio de Janeiro, COPPE LTTC, Dep Mech Engn, BR-21945970 Rio De Janeiro, Brazil. [Pachepsky, Y. A.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Nicholson, T. J.; Cady, R. E.] US NRC ORR, US Nucl Regulatory Commiss, Rockville, MD USA. RP Gish, TJ (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM Timothy.Gish@ARS.USDA.GOV RI Simunek, Jiri/F-3196-2011; van Genuchten, Martinus/K-6892-2013 OI van Genuchten, Martinus/0000-0003-1654-8858 FU Interagency USDA; U.S. NRC [RES-02-008] FX This work has been partially supported through the Interagency USDA and U.S. NRC Agreement RES-02-008 "Model Abstraction Techniques for Soil Water Flow and Transport." NR 40 TC 7 Z9 7 U1 0 U2 5 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2010 VL 9 IS 3 BP 686 EP 696 DI 10.2136/vzj2009.0054 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 678FE UT WOS:000284058700017 ER PT J AU Goldberg, S Kabengi, NJ AF Goldberg, Sabine Kabengi, Nadine J. TI Bromide Adsorption by Reference Minerals and Soils SO VADOSE ZONE JOURNAL LA English DT Article ID ALUMINA ELECTROLYTE INTERFACE; ELECTRICAL DOUBLE-LAYER; TRANSPORT; TRACERS; CHARGE; IONS; RETENTION; EXCHANGE; MODELS; OXIDES AB Bromide, Br-, adsorption behavior was investigated on amorphous Al and Fe oxide, montmorillonite, kaolinite, and temperate and tropical soils. Bromide adsorption decreased with increasing solution pH with minimal adsorption occurring above pH 7. Bromide adsorption was higher for amorphous oxides than for clay minerals. Shifts in point of zero charge (PZC) were observed on amorphous Al and Fe oxide following Br-adsorption, suggesting an inner-sphere adsorption mechanism for Br- on these surfaces. Ionic strength effects indicated an inner-sphere adsorption mechanism for Br- on kaolinite and an outer-sphere adsorption mechanism on amorphous Fe oxide. Two chemical surface complexation models, the constant capacitance model and the triple layer model, were able to describe Br-adsorption as a function of solution pH on all materials. For the oxides and clay minerals and most of the soils the fit of the constant capacitance model, containing an inner-sphere adsorption mechanism, was improved over that of the triple layer model, containing an outer-sphere adsorption mechanism, as measured by the overall variance, V-gamma. Bromide adsorption on amorphous Fe oxide as a function of solution pH and solution ionic strength was well described using the triple layer model. Our results indicate that Br- would most likely not act as a conservative tracer at soil solution pH values below 7. Therefore, we suggest that researchers carefully evaluate the pH regime and mineralogy of their study site before assuming that Br- can be applied as a conservative tracer for transport experiments. C1 [Goldberg, Sabine] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Kabengi, Nadine J.] Univ Kentucky, Plant & Soil Sci Dep, Lexington, KY 40546 USA. RP Goldberg, S (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Sabine.Goldberg@ars.usda.gov NR 35 TC 12 Z9 12 U1 1 U2 8 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD AUG PY 2010 VL 9 IS 3 BP 780 EP 786 DI 10.2136/vzj2010.0028 PG 7 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 678FE UT WOS:000284058700025 ER PT J AU Zhang, L Seagren, EA Davis, AP Karns, JS AF Zhang, Lan Seagren, Eric A. Davis, Allen P. Karns, Jeffrey S. TI The Capture and Destruction of Escherichia coli from Simulated Urban Runoff Using Conventional Bioretention Media and Iron Oxide-coated Sand SO WATER ENVIRONMENT RESEARCH LA English DT Article DE stormwater; bioretention; iron-oxide coated sand; Escherichia coli; bacterial capture; survival; protozoa; predation ID OXYHYDROXIDE GRAIN COATINGS; SATURATED POROUS-MEDIA; CRYPTOSPORIDIUM-PARVUM; ESTUARINE WATERS; BACTERIAL-CELLS; IONIC-STRENGTH; TRANSPORT; SOIL; SURVIVAL; PROTOZOA AB The performance, sustainability, and mechanisms of bacterial removal from stormwater runoff by bioretention systems are poorly understood. The potential for removal of microorganisms in bioretention systems was evaluated using column studies and simulated urban stormwater runoff. Conventional bioretention media (CBM) removed 82% of Escherichia coli O157:H7 strain B6914 cells; iron-oxide coated sand (IOCS) significantly enhanced capture, with 99% efficiency. This improvement possibly was because of the greater positive surface charge and roughness of the IOCS. Trapped strain B6914 cells decayed more rapidly in CBM, however, with more than 99.98% die-off within one week compared with the IOCS in which approximately 48% of trapped cells survived. Predation and competition from native microorganisms in CBM were verified to play a dominant role in rapid destruction of trapped strain B6914. In particular, protozoan grazing appeared to play an important role, with the die-off of trapped B6914 increasing with increasing concentrations of protozoa. Water Environ. Res., 82, 701 (2010). C1 [Zhang, Lan; Davis, Allen P.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Seagren, Eric A.] Michigan Technol Univ, Dept Civil & Environm Engn, Houghton, MI 49931 USA. [Karns, Jeffrey S.] ARS, USDA, Beltsville, MD USA. RP Zhang, L (reprint author), Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. EM eseagren@mtu.edu RI DAVIS, ALLEN/F-1066-2017 OI DAVIS, ALLEN/0000-0001-7818-1890 FU Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET); National Oceanic and Atmospheric Administration [NA05NOS4191149] FX This work was supported by the Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET), National Oceanic and Atmospheric Administration grant number NA05NOS4191149. The authors thank Dr. Mohamad Al-Sheikhly (University of Maryland) and Dr. Otto Wilson (Catholic University of America) for providing laboratory equipment and technical support. NR 76 TC 25 Z9 26 U1 1 U2 35 PU WATER ENVIRONMENT FEDERATION PI ALEXANDRIA PA 601 WYTHE ST, ALEXANDRIA, VA 22314-1994 USA SN 1061-4303 J9 WATER ENVIRON RES JI Water Environ. Res. PD AUG PY 2010 VL 82 IS 8 BP 701 EP 714 DI 10.2175/106143010X12609736966441 PG 14 WC Engineering, Environmental; Environmental Sciences; Limnology; Water Resources SC Engineering; Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 630UN UT WOS:000280300300004 PM 20853749 ER PT J AU Shivrain, VK Burgos, NR Agrama, HA Lawton-Rauh, A Lu, B Sales, MA Boyett, V Gealy, DR Moldenhauer, KAK AF Shivrain, V. K. Burgos, N. R. Agrama, H. A. Lawton-Rauh, A. Lu, B. Sales, M. A. Boyett, V. Gealy, D. R. Moldenhauer, K. A. K. TI Genetic diversity of weedy red rice (Oryza sativa) in Arkansas, USA SO WEED RESEARCH LA English DT Article DE crop-weed hybridisation; indica rice; gene flow; genotype; rice; tropical japonica rice; simple sequence repeat; SSR ID CLEARFIELD(TM) RICE; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; OUTCROSSING RATE; COMMERCIAL RICE; WILD RELATIVES; FLOW; CULTIVARS; PATTERNS; ORIGIN AB P>Weedy red rice (Oryza sativa) is a problematic weed in cultivated rice. About 50% of US rice is produced in Arkansas and 60% of these fields have some red rice infestation. Red rice populations are morphologically and phenologically diverse. We hypothesise that red rice in Arkansas has high genetic diversity, which underlies its wide phenotypic diversity, and that some alleles from cultivated rice have been introgressed into red rice during more than a century of coexistence. We tested 137 red rice accessions from four ecological zones in Arkansas and 36 cultivars that have been grown in Arkansas in the past century. Twenty-seven rice microsatellite primers, distributed across 12 chromosomes, were used to generate molecular markers. The overall Nei's genetic distance (GD) of red rice accessions was 0.70. Rice grown in the last century had an overall GD of 0.26. The awnless strawhull red rice was genetically distant from blackhull (GD = 0.55) and brownhull (GD = 0.60) red rice types. Nei's GD between blackhull and brownhull red rice was 0.42. Brownhull and blackhull formed one genotypic cluster, whereas the majority of strawhull red rice formed another cluster. Within blackhull red rice, the GD was 0.76, whereas for awnless strawhull it was 0.68, 0.75 for awned strawhull and 0.80 for brownhull types. The gene diversity of blackhull and strawhull correlated with zone of origin. A quarter of the red rice accessions share common alleles with cultivated rice. A diverse complex of weedy populations has evolved in a region devoid of other weedy and wild Oryza species. C1 [Shivrain, V. K.; Burgos, N. R.; Sales, M. A.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Agrama, H. A.; Gealy, D. R.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Lawton-Rauh, A.] Clemson Univ, Dept Biochem & Genet, Clemson, SC USA. [Lu, B.] Fudan Univ, Inst Biodivers Sci, Shanghai 200433, Peoples R China. [Boyett, V.; Moldenhauer, K. A. K.] Rice Res & Extens Ctr, Stuttgart, AR USA. RP Burgos, NR (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, 1366 W Altheimer Dr, Fayetteville, AR 72704 USA. EM nburgos@uark.edu RI Lu, Bao-Rong/C-3130-2009; OI Lu, Bao-Rong/0000-0002-0214-2390; Lawton-Rauh, Amy/0000-0003-4610-7367 NR 43 TC 24 Z9 28 U1 1 U2 24 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0043-1737 J9 WEED RES JI Weed Res. PD AUG PY 2010 VL 50 IS 4 BP 289 EP 302 DI 10.1111/j.1365-3180.2010.00780.x PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 620YO UT WOS:000279539400002 ER PT J AU Mirsky, SB Gallandt, ER Mortensen, DA Curran, WS Shumway, DL AF Mirsky, S. B. Gallandt, E. R. Mortensen, D. A. Curran, W. S. Shumway, D. L. TI Reducing the germinable weed seedbank with soil disturbance and cover crops SO WEED RESEARCH LA English DT Article DE cover crop; seedbank; sustainable agriculture; population dynamics; mechanical weed control; Chenopodium album; Abutilon theophrasti; Setaria ID ORGANIC-MATTER; MANAGEMENT; TILLAGE; DYNAMICS; SYSTEMS; SEEDS; COMMUNITIES; RESPONSES; MOVEMENT; LIGHT AB P>Ecologically based weed management relies heavily on a greater integration of cultural and mechanical control tactics than conventional weed management. As such, management outcomes are more dependent on biotic interactions among the crop, weed and methods of management. In this study, we assessed the influence of soil disturbance and cover cropping on the germinable seedbank of Chenopodium album, Abutilon theophrasti and Setaria spp. across a range of initial weed seedbank densities. Cover crop system treatments ranged from greater reliance on tillage at one extreme, to a greater reliance on cover cropping to accomplish weed suppression at the other. In general, seedbank losses to germination were highest when soil disturbance was associated with cover cropping. Of the five cover crop systems (1 year duration), the summer fallow and the yellow mustard/buckwheat/winter rape mixture consistently decreased the weed seedbank. The magnitude of decline in these high disturbance systems reached complete depletion of the Setaria spp. germinable seedbank and an 85% and 80% reduction for C. album and A. theophrasti respectively. Both systems included tilling the soil three to four times throughout the growing season. In contrast, treatments which involved less soil disturbance had weed escapes that set seed in one or more site years. Cover crop systems that stimulated weed seed germination and where weeds were either suppressed by the cover crop or where subsequent tillage provided control, resulted in the greatest weed seedbank declines. C1 [Mirsky, S. B.] ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD 20705 USA. [Gallandt, E. R.] Univ Maine, Dept Plant Soil & Environm Sci, Orono, ME USA. [Mortensen, D. A.; Curran, W. S.] Penn State Univ, Dept Crop & Soil Sci, University Pk, PA 16802 USA. [Shumway, D. L.] Penn State Univ, Dept Stat, University Pk, PA 16802 USA. RP Mirsky, SB (reprint author), ARS, Sustainable Agr Syst Lab, USDA, 10300 Baltimore Ave,Bldg 001,Rm 117,BARC W, Beltsville, MD 20705 USA. EM steven.mirsky@ars.usda.gov NR 35 TC 18 Z9 19 U1 6 U2 48 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0043-1737 J9 WEED RES JI Weed Res. PD AUG PY 2010 VL 50 IS 4 BP 341 EP 352 DI 10.1111/j.1365-3180.2010.00792.x PG 12 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 620YO UT WOS:000279539400007 ER PT J AU Jakubowski, AR Casler, MD Jackson, RD AF Jakubowski, Andrew R. Casler, Michael D. Jackson, Randall D. TI Landscape Context Predicts Reed Canarygrass Invasion: Implications for Management SO WETLANDS LA English DT Article DE Habitat models; Invasive species; Nutrient management; Phalaris arundinacea; Restoration; Wetlands ID GRASS PHALARIS-ARUNDINACEA; PLANT INVASIONS; REGRESSION TREES; SIMULATION-MODEL; CLASSIFICATION; L.; ESTABLISHMENT; CONSERVATION; POPULATION; ECOSYSTEMS AB Understanding the landscape distribution of invasive species has become an important tool to help land managers focus their efforts. We used land cover data to predict the proportion of wetlands in a watershed dominated by reed canarygrass (Phalaris arundinacea L.), one of the most dominant wetland invaders in North America over the past century. Our results indicated that the landscape configuration of a watershed was a better predictor than the landscape composition of a watershed, with the adjacency of wetlands to agriculture and open water identified as the best predictors of the proportion of wetlands in a watershed dominated by reed canarygrass. In contrast, proportion of agriculture and open water were identified as the next best predictors in our regression tree, but explained significantly less variability. These results suggest that the risk of invasion by reed canarygrass varies among watersheds, and further that the potential for restoration success may similarly vary across the landscape. We argue that it is essential to understand the landscape context of a wetland before attempting a restoration project because success may be mediated by factors outside the local site. C1 [Jakubowski, Andrew R.; Jackson, Randall D.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Jakubowski, AR (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM Jakubowski@wisc.edu RI Jakubowski, Andrew/F-4905-2010 NR 44 TC 9 Z9 9 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD AUG PY 2010 VL 30 IS 4 BP 685 EP 692 DI 10.1007/s13157-010-0078-y PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 646TG UT WOS:000281565300004 ER PT J AU Ehrlich, KC Wei, Q Bhatnagar, D AF Ehrlich, K. C. Wei, Q. Bhatnagar, D. TI Increased sensitivity of Aspergillus flavus and Aspergillus parasiticus aflatoxin biosynthesis polyketide synthase mutants to UVB light SO WORLD MYCOTOXIN JOURNAL LA English DT Article DE aflatoxin biosynthesis; biocontrol; fungal competition; gene disruption; fungal spores ID FUNGUS METARHIZIUM-ANISOPLIAE; BIOLOGICAL-CONTROL; SECONDARY METABOLISM; STERIGMATOCYSTIN BIOSYNTHESIS; VEGETATIVE COMPATIBILITY; CYCLOPIAZONIC ACID; GENE-CLUSTER; BARLEY-GRAIN; B-1; CONTAMINATION AB One strategy to reduce aflatoxin contamination of maize and cottonseed is to introduce spores of non-aflatoxigenic strains as competitors. Using isogenic mutants we show that, upon 5 or 20 min exposure to 302 nm (UVB) light, the viability of conidia of Aspergillus flavus and Aspergillus parasiticus mutants lacking the ability to accumulate any aflatoxin precursor metabolite is reduced five-fold compared to that of aflatoxin-producing strains or pigmented mutants that accumulate aflatoxin precursors. This result suggests that the long-term viability of introduced non-aflatoxigenic competitor strains may be lower than that of natural aflatoxin-producing isolates when exposed to sunlight. C1 [Ehrlich, K. C.; Wei, Q.; Bhatnagar, D.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Ehrlich, KC (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM ken.ehrlich@ars.usda.gov NR 48 TC 4 Z9 4 U1 1 U2 7 PU WAGENINGEN ACAD PUBL PI BILTHOVEN PA PO BOX 179, BILTHOVEN, 3720 AD, NETHERLANDS SN 1875-0710 J9 WORLD MYCOTOXIN J JI World Mycotoxin J. PD AUG PY 2010 VL 3 IS 3 BP 263 EP 270 DI 10.3920/WMJ2010.1218 PG 8 WC Food Science & Technology; Mycology; Toxicology SC Food Science & Technology; Mycology; Toxicology GA 664XC UT WOS:000282996200007 ER PT J AU Peppin, D Fule, PZ Sieg, CH Beyers, JL Hunter, ME AF Peppin, Donna Fule, Peter Z. Sieg, Carolyn Hull Beyers, Jan L. Hunter, Molly E. TI Post-wildfire seeding in forests of the western United States: An evidence-based review SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Systematic review; Post-wildfire rehabilitation; Plant community recovery; Soil stabilization; Invasive species ID PLANT INVASIONS; FIRE SEVERITY; SIERRA-NEVADA; STRAW MULCH; CONSERVATION; VEGETATION; RECOVERY; COLORADO; IMPACTS; EROSION AB Broadcast seeding is one of the most widely used post-wildfire emergency response treatments intended to reduce soil erosion, increase vegetative ground cover, and minimize establishment and spread of non-native plant species. We conducted an evidence-based review to examine the effectiveness and effects of post-wildfire seeding treatments on soil stabilization, non-native species invasion, and plant community recovery in the western U.S. We reviewed 94 scientific papers and agency monitoring reports identified using a systematic search protocol. As sampling designs have become more rigorous in recent years, evidence that seeding is effective in reducing erosion has decreased. Of highest and high quality studies evaluating soil erosion, 92% (11 of 12) were published since 2000, none of which showed an effective result. Before 2000, the majority of the studies (71%) fell into the lowest quality categories, of which 72% showed seeding to be effective. The majority of studies (20 of 27, 74%) evaluating soil erosion in seeded versus unseeded controls showed that seeding did not reduce erosion relative to unseeded controls. Even when seeding significantly increased vegetative cover, seeded sites rarely supported plant cover levels considered sufficient to stabilize soils within the first and second year post-wildfire. Of the 11 studies evaluating seeding effectiveness for curtailing invasions of non-native plant species, an almost equal percentage found seeding treatments to be either effective (54%, 6 studies) or ineffective (45%, 5 studies). However, the majority of effective and ineffective treatments (83% and 80%, respectively) used non-native species. Sixteen of 26 studies (62%) evaluating seeding effects on plant communities reported that seeding suppressed recovery of native plants, although data on long-term impacts of this reduction are limited. The literature suggests that post-wildfire seeding does little to protect soil in the short-term, has equivocal effect on invasion of non-native species, and can have negative effects on native vegetation recovery, although long-term studies are needed to assess lasting impacts of seeded species. (C) 2010 Elsevier B.V. All rights reserved. C1 [Peppin, Donna; Fule, Peter Z.; Hunter, Molly E.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Peppin, Donna; Fule, Peter Z.; Hunter, Molly E.] No Arizona Univ, Ecol Restorat Inst, Flagstaff, AZ 86011 USA. [Sieg, Carolyn Hull] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff Lab, Flagstaff, AZ 86001 USA. [Beyers, Jan L.] US Forest Serv, USDA, Pacific SW Res Stn, Forest Fire Lab, Riverside, CA 92507 USA. RP Fule, PZ (reprint author), No Arizona Univ, Sch Forestry, 200 E Pine Knoll Dr,Room 116, Flagstaff, AZ 86011 USA. EM donna.peppin@nau.edu; Pete.Fule@nau.edu; csieg@fs.fed.us; jbeyers@fs.fed.us; Molly.Hunter@nau.edu FU Joint Fire Science Program (JFSP) [08-2-1-11] FX This research was supported by a grant from Joint Fire Science Program (JFSP Project #08-2-1-11). We thank the students of the Ecological Restoration Institute at Northern Arizona University who helped establish the publication database of relevant post-wildfire seeding publications to synthesize. NR 73 TC 39 Z9 39 U1 3 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 573 EP 586 DI 10.1016/j.foreco.2010.06.004 PG 14 WC Forestry SC Forestry GA 638AH UT WOS:000280861200001 ER PT J AU Palik, BJ Kastendick, D AF Palik, Brian J. Kastendick, Doug TI Response of seasonal pond plant communities to upland forest harvest in northern Minnesota forests, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Seasonal ponds; Vernal pools; Forest wetlands; Wetland plant communities; Harvesting impacts ID ISOLATED WETLANDS; ECOTOXICOLOGICAL FIELD; LEVEL ANALYSIS; VERNAL POOLS; DYNAMICS; MACROINVERTEBRATES; CONSERVATION; REGENERATION; CURVES; STREAM AB Small seasonally flooded forest ponds have received increased attention due to a growing recognition of their abundance in many landscapes, their importance as habitat for a variety of organisms, and the contributions they make to species and ecosystem diversity. There also is concern over potential negative effects of forest management in adjacent uplands on seasonal pond ecology. Several studies have examined invertebrate and songbird responses to upland harvest around seasonal ponds. Less attention has been given to examining how seasonal pond plant communities respond to adjacent forest harvesting. We studied the response of seasonal pond plant communities to adjacent upland timber harvests, assessing whether buffers around ponds (15.25 m uncut and partially cut) mitigated changes in species abundance and community composition, relative to changes in ponds that were clearcut to the pond margin. We addressed our objective using an operational-scale experiment in northern Minnesota, which included pre-harvest sampling, replicated treatments, and uncut controls. After treatment, changes in tree basal area and canopy openness in the pond basins reflected reductions in upland basal areas. Specifically, control ponds had significantly higher basal area and lower openness than ponds cut to their margins, while ponds with uncut buffers and partially cut buffers were intermediate. Changes in plant communities were evident in the ground layer and shrub/large regeneration layer. After treatment and over time, the control stands did not change significantly in ground layer structure or shrub/large regeneration layer composition. The three upland harvest treatments displayed increasingly greater deviation from their starting conditions and from the control along a gradient of increasing treatment intensity, from the buffer treatment to the partially cut buffer to the clearcut. The response in the ground layer was largely associated with increased sedge and grass cover, while the response in the shrub/large regeneration layer was associated with increases of Salix sp., Alnus incana, and Populus tremuloides. Our results indicate that adjacent upland timber harvest can lead to altered plant communities within seasonal ponds, at least temporarily. Moreover, uncut forest buffers (similar to 15.25 m) surrounding seasonal ponds can mitigate plant community changes to some degree. If seasonal ponds are an important resource on the management landscape and a high percentage of upland forest is in a recently cut condition at any given time, than use of harvest buffers around seasonal ponds may be an appropriate approach for mitigating short term alteration of pond plant communities. Published by Elsevier B.V. C1 [Palik, Brian J.; Kastendick, Doug] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Palik, BJ (reprint author), US Forest Serv, USDA, No Res Stn, 1831 Highway 169 E, Grand Rapids, MN 55744 USA. EM bpalik@fs.fed.us; dkastendick@fs.fed.us FU USDA Forest Service; Northern Research Station; USDA FS Washington Office FX This research was supported by the USDA Forest Service, Northern Research Station, and the USDA FS Washington Office, through a Presidential Early Career Scientist Award to B. Palik. Extensive logistical support and access to study sites was provided by Potlatch Corporation and the Cass County, MN Land Department. We thanks the numerous technicians and students collected and processed data for this project especially Dwight Streblow, Leanne Egland, and Cory Cease. Randy Kolka and Mark Hanson provided comments on the manuscript. NR 50 TC 3 Z9 3 U1 3 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 628 EP 637 DI 10.1016/j.foreco.2010.05.019 PG 10 WC Forestry SC Forestry GA 638AH UT WOS:000280861200007 ER PT J AU Worrall, JJ Marchetti, SB Egeland, L Mask, RA Eager, T Howell, B AF Worrall, James J. Marchetti, Suzanne B. Egeland, Leanne Mask, Roy A. Eager, Thomas Howell, Brian TI Effects and etiology of sudden aspen decline in southwestern Colorado, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Decline; Dieback; Mortality; Drought; Climate change; Populus tremuloides ID DROUGHT; REGENERATION; DIEBACK; FORESTS AB Sudden aspen decline (SAD), affecting Populus tremuloides, was first observed in Colorado in 2004. By 2008 it affected at least 220,000 ha, an estimated 17% of the aspen cover type in the state. In southwestern Colorado, we examined site and stand features in paired healthy and damaged plots to assess the effects of SAD on aspen and to identify factors associated with decline. Root mortality increased significantly with recent crown loss. Consequently, density of regeneration did not increase as the overstory deteriorated, and regeneration that originated since 2002 decreased significantly in stands with moderate to severe SAD. However, mortality of regeneration did not increase with that of the overstory. Remeasurement of a subset of plots after 1-2 yrs showed significant increases in severity. Contrary to expectations, overstory age and diameter were not related to SAD severity as measured by recent crown loss or mortality. Severity of SAD was inversely, but weakly, related to basal area, stem slenderness, and site index, and positively related to upper slope positions. This is consistent with moisture stress as an underlying factor. To test the role of climate as an inciting factor for SAD, a landscape-scale climate model was used to compare moisture status of declining and healthy aspen at the height of the warm drought in water year 2002. Polygons identified as damaged aspen in the 2008 aerial survey had greater moisture deficits than healthy aspen in the 2002 water year. SAD has led to loss of aspen cover in some stands, and is occurring in areas where early loss of aspen due to climate change has been predicted. Further warm, dry growing seasons will likely lead to recurrence of SAD. Published by Elsevier B.V. C1 [Worrall, James J.; Marchetti, Suzanne B.; Egeland, Leanne; Mask, Roy A.; Eager, Thomas] US Forest Serv, USDA, Gunnison, CO 81230 USA. [Howell, Brian] US Forest Serv, USDA, Golden, CO 80401 USA. RP Worrall, JJ (reprint author), US Forest Serv, USDA, 216 N Colorado St, Gunnison, CO 81230 USA. EM jworrall@fs.fed.us RI Worrall, James/G-9496-2011 FU USDA Forest Service FX This work was supported in part by the USDA Forest Service, Forest Health Monitoring, Evaluation Monitoring Program. Much of the field work was ably conducted by K.B. Kemp, S.C. Tharp, A.F. Watkins, B.C. Cochran, and J.S. Pratt. Assistance with constructing an equation for calculating CMI and a model for analyzing it in a GIS system was provided by E.H. Hogg, R.C. Venette, and D. Armlovich. W.D. Shepperd provided helpful advice on the sampling methods as well as general guidance and encouragement. D.A. Dearstyne provided advice and training of crew members on soil field assessment, classification and data analysis. J. Scott and T. Hughes also provided guidance and crew training in soil assessment. B.C. Johnston provided assistance in plant identification. Aerial survey was conducted by the US Forest Service with assistance from the Colorado State Forest Service and contractors. J.S. Ross provided aerial survey data summaries and GIS products. J.W. Worrall provided a derivation of the formula for calculating root length per unit area from the root transects. Presubmission reviews were kindly provided by W.D. Shepperd, W.R. Jacobi, and D. Binkley. NR 37 TC 82 Z9 83 U1 2 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 638 EP 648 DI 10.1016/j.foreco.2010.05.020 PG 11 WC Forestry SC Forestry GA 638AH UT WOS:000280861200008 ER PT J AU Svoboda, M Fraver, S Janda, P Bace, R Zenahlikova, J AF Svoboda, Miroslav Fraver, Shawn Janda, Pavel Bace, Radek Zenahlikova, Jitka TI Natural development and regeneration of a Central European montane spruce forest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bark beetle; Competition; Dendrochronology; Dead wood; Stem-mapped plots; Wind disturbance ID PICEA-ABIES FOREST; COARSE WOODY DEBRIS; BEETLE IPS-TYPOGRAPHUS; EASTERN ITALIAN ALPS; NORWAY SPRUCE; SOUTHERN FINLAND; WIND DISTURBANCE; MOUNTAIN FORESTS; BOHEMIAN FOREST; GAP DISTURBANCE AB Montane Norway spruce forests of Central Europe have a very long tradition of use for timber production; however, recently there has been increasing concern for their role in maintaining biological diversity. This concern, coupled with recent severe windstorms that led to wide-spread bark beetle outbreaks, has brought the management of montane spruce forests to the forefront of public policy discussions in Central Europe. In order to shed light on the natural development and current structure of mature montane spruce forests, we established four 0.25 ha research plots in a semi-natural montane spruce forest in the Sumava Mountains (The Bohemian Forest), Czech Republic. We mapped all trees, extracted increment cores for age and growth-pattern analyses, and inventoried all current tree regeneration, including the substrates on which it was found. Stands were characterized by uni-modal tree diameter distributions and high basal areas (56.6 m(2) ha(-1) on average), indicating a natural transition from the stem exclusion phase towards the understory reinitiation phase. The stands showed largely single-cohort recruitment age structures, however, with recruitment spanning seven decades. Our analyses suggest that this cohort existed as advance regeneration prior to major disturbances in the late 1800s, which included post-bark beetle salvage logging. Spatial pattern analyses of living and dead stems combined, showed an increase in uniformity of living trees, pointing to the role of natural density-dependent mortality. However, past growth patterns and historical documentation suggest that low intensity canopy disturbances (wind and snow) also caused mortality and diversified canopy structure. Because the stands developed naturally over the past 120+ years and thus escaped thinning operations, high volumes of coarse woody debris (94 m(3) ha(-1)) and snag densities (546 stems ha(-1)) have accrued. Advance spruce regeneration was quite abundant and existed primarily on deadwood substrates, even though these occupied only a small percent of stand area. Because of salvage logging in the late 1880s, these stands do not qualify, according to the traditional paradigm, as natural spruce forests. As a result, they are recently subject to active management practices including salvage logging that remove dead and dying trees. Given the importance of deadwood for forest regeneration and recovery from disturbance, as demonstrated in this study, we argue that dead wood removal may limit future natural regeneration in these stands. Thus, the purported benefits of removing dead and dying trees from semi-natural forests must be carefully weighed against the potential detrimental impacts on natural spruce forest regeneration and biodiversity. (C) 2010 Elsevier B.V. All rights reserved. C1 [Svoboda, Miroslav; Janda, Pavel; Bace, Radek; Zenahlikova, Jitka] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Prague 16521 6, Suchdol, Czech Republic. [Fraver, Shawn] US Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Svoboda, M (reprint author), Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Kamycka 129, Prague 16521 6, Suchdol, Czech Republic. EM svobodam@fld.czu.cz; sfraver@fs.fed.us; janda@fld.czu.cz; bace@fld.czu.cz; zenahlikova@fld.czu.cz RI Svoboda, Miroslav/E-6860-2010; Bace, Radek/N-2228-2014; OI Svoboda, Miroslav/0000-0003-4050-3422; Janda, Pavel/0000-0003-4732-6908 FU Czech Science Foundation [P504/10/1644, P504/10/0843] FX This study was supported by the Czech Science Foundation, project no. P504/10/1644 and project no. P504/10/0843. We would like to thank A. Husnik, K. Nechuta, L Bilek, and S. Hofmeister for assistance in the field. We are especially grateful to J. Wild, M. Jonasova, M. Kopecky, T. Nagel, D. Hardekopf, and K. Edwards for providing valuable comments on earlier drafts of the manuscript. Comments by anonymous reviewers have greatly improved the manuscript. We thank the Sumava National Park authorities for administrative support. NR 85 TC 52 Z9 56 U1 10 U2 91 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 707 EP 714 DI 10.1016/j.foreco.2010.05.027 PG 8 WC Forestry SC Forestry GA 638AH UT WOS:000280861200014 ER PT J AU Reo, NJ Karl, JW AF Reo, Nicholas J. Karl, Jason W. TI Tribal and state ecosystem management regimes influence forest regeneration SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Oak regeneration; Northern red oak; Tribal natural resource management; Ecosystem management; White-tailed deer; Herbivory ID NORTHERN RED OAK; DEER OVERABUNDANCE; SAPLING GROWTH; QUERCUS-RUBRA; DYNAMICS; COMMUNITIES; SEEDLINGS; MICHIGAN; LIGHT; GAPS AB Wild ungulates such as white-tailed deer (Odocoileus virginianus) are highly valued wildlife assets that provide subsistence, economic and cultural benefits to hunters and rural communities. Yet, high density populations of these herbivores can contribute significantly to regeneration failures in a wide range of forest types. Pre-European settlement white-tailed deer densities were estimated to have been approximately 2-4 deer km(-2), and similar densities have been recommended to balance contemporary forest regeneration and wildlife objectives. We studied northern red oak (Quercus rubra L.) regeneration on neighboring tribal and state forests where socio-cultural differences have led to distinct hunting management practices and subsequent differences in wildlife-plant interactions. Tribes such as the Lac du Flambeau Chippewa have kept deer populations relatively low on reservation lands through active hunting practices. We used an observational study approach to compare in situ ungulate herbivory under low (2-3 deer km(-2)) and high (>10 deer km(-2)) population densities. We measured northern red oak regeneration on tribal and state forests in two management unit types: contiguous stands of oak >15 ha in area and small residual "pockets" of oak <3 ha left by foresters as a source of seed and wildlife mast. Herbivory levels were significantly higher on state forests than tribal forests and were closely correlated with the density of larger seedlings, particularly in oak pockets. If herbivory levels are too high, even with adequate light, our results suggest that seedlings may not survive in densities sufficient to maintain northern red oak as a co-dominant species in mixed forests. However, when deer densities are kept at 2-4 deer km(-2), our results suggest that northern red oak seedlings can survive beyond browseable heights in sufficient numbers for maintaining oak. Tribal lands can provide contemporary examples of longstanding low to intermediate deer densities and sustainable deer-forest relationships. (C) 2010 Elsevier B.V. All rights reserved. C1 [Reo, Nicholas J.] Michigan State Univ, Native Amer Inst, Coll Agr & Nat Resources, E Lansing, MI 48824 USA. [Reo, Nicholas J.] Michigan State Univ, Dept Fisheries & Wildlife, Ctr Syst Integrat & Sustainabil, E Lansing, MI 48824 USA. [Karl, Jason W.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. RP Reo, NJ (reprint author), Michigan State Univ, Native Amer Inst, Coll Agr & Nat Resources, 412 Agr Hall, E Lansing, MI 48824 USA. EM reo@msu.edu OI Reo, Nicholas/0000-0001-8839-323X; Karl, Jason/0000-0002-3326-3806 FU Ford Foundation; National Science Foundation; NASA; Michigan State University FX Financial support for this project was provided by the Ford Foundation, the National Science Foundation, and NASA. We thank state and tribal managers and tribal leaders from our study site for allowing and helping us conduct the research; Stuart Burgess and Donald Johnson for invaluable assistance with field work; Angela Spickard for ongoing assistance and support in all project stages; Henry Kovacs for geospatial technical assistance; Dan Kramer, Mike Walters, George Cornell, Burton Barnes and Dan Kashian for research guidance and review of this manuscript; and Jianguo Liu and members of the Center for Systems Integration and Sustainability at Michigan State University for valuable project support and feedback. NR 48 TC 0 Z9 0 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 734 EP 743 DI 10.1016/j.foreco.2010.05.030 PG 10 WC Forestry SC Forestry GA 638AH UT WOS:000280861200017 ER PT J AU Hood, SM Smith, SL Cluck, DR AF Hood, Sharon M. Smith, Sheri L. Cluck, Daniel R. TI Predicting mortality for five California conifers following wildfire SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Logistic regression; Bark beetle; Crown scorch; Cambium kill; Crown kill ID TREE MORTALITY; PONDEROSA PINE; PRESCRIBED-FIRE; SIERRA-NEVADA; DOUGLAS-FIR; POSTFIRE SURVIVAL; FOREST STRUCTURE; BEETLE ATTACKS; CROWN SCORCH; DAMAGE AB Fire injury was characterized and survival monitored for 5677 trees >25 cm DBH from five wildfires in California that occurred between 2000 and 2004. Logistic regression models for predicting the probability of mortality 5-years after fire were developed for incense cedar (Calocedrus decurrens (Torr.) Florin), white fir (Abies concolor (Gord. & Glend.) Lindl. ex Hildebr.), sugar pine (Pinus lambertiana Douglas), Jeffrey pine (P. jeffreyi Balf.), and ponderosa pine (P. ponderosa C. Lawson). Differences in crown injury variables were also compared for Jeffrey and ponderosa pine. Most mortality (70-88% depending on species) occurred within 2 years post-wildfire and had stabilized by year 3. Crown length and crown volume injury variables predicted tree mortality equally well; however, the variables were not interchangeable. Crown injury and cambium kill rating was significant in predicting mortality in all models. DBH was only a significant predictor of mortality for white fir and the combined ponderosa and Jeffrey pine models developed from the McNally Fire; these models all predicted increasing mortality with increasing tree size. Red turpentine beetle (Dendroctonus valens) was a significant predictor variable for sugar pine, ponderosa pine, and Jeffrey pine; ambrosia beetle (Trypodendron and Gnathotrichus spp.) was a significant predictor variable for white fir. The mortality models and post-fire tree survival characteristics provide improved prediction of 5-year post-wildfire tree mortality for several California conifers. The models confirm the overall importance of crown injury in predicting post-fire mortality compared to other injury variables for all species. Additional variables such as cambium kill, bark beetles, and tree size improved model accuracies, but likely not enough to justify the added expense of data collection. Published by Elsevier B.V. C1 [Hood, Sharon M.] US Forest Serv, USDA, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. [Smith, Sheri L.; Cluck, Daniel R.] US Forest Serv, USDA, Susanville, CA 96130 USA. RP Hood, SM (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Fire Sci Lab, 5775 Highway 10W, Missoula, MT 59808 USA. EM shood@fs.fed.us RI Hood, Sharon/E-5209-2015 OI Hood, Sharon/0000-0002-9544-8208 FU Joint Fire Science Program [JFSP 05-2-1-105]; Tahoe National Forests FX The Forest Service, U.S. Department of Agriculture, Region 5, Natural Resource Management program and Forest Health Protection program provided funding for data collection and analysis. We also acknowledge funding from the Joint Fire Science Program under Project JFSP 05-2-1-105. We greatly appreciate the support and cooperation from personnel on the Eldorado, Lassen, Sequoia and Tahoe National Forests. Kevin Ryan assisted with the study design and Dave Turner provided statistical advice. We thank M. Ahearn, M. Berry, K. Bowdoin, J. Egan, S. Emmert, C. Evanoff, E. Frolli, L. Galland, S. Hack, R. Mahnke, S. McFarland, J. Morris, M. Murphy, S. Muse, D. Olson, K. Patterson, J. Paul, K. Pavlica, E. Smith, B. Strom, and R. Turcotte for their assistance with field data collection and J. Maddox and R. Bennett for data entry. The comments of Charles McHugh and two anonymous reviewers greatly improved previous versions of this manuscript. NR 74 TC 16 Z9 16 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 750 EP 762 DI 10.1016/j.foreco.2010.05.033 PG 13 WC Forestry SC Forestry GA 638AH UT WOS:000280861200019 ER PT J AU Hurteau, MD North, M AF Hurteau, Matthew D. North, Malcolm TI Carbon recovery rates following different wildfire risk mitigation treatments SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carbon; Wildfire; Fuels treatment; Climate change; Mitigation ID MIXED-CONIFER FOREST; POTENTIAL FIRE BEHAVIOR; NORTHERN SIERRA-NEVADA; PONDEROSA PINE FOREST; CLIMATE-CHANGE; RESTORATION TREATMENTS; THINNING TREATMENT; PRESCRIBED FIRE; STAND DENSITY; UNITED-STATES AB Sequestered forest carbon can provide a climate change mitigation benefit, but in dry temperate forests, wildfire poses a reversal risk to carbon offset projects. Reducing wildfire risk requires a reduction in and redistribution of carbon stocks, the benefit of which is only realized when wildfire occurs. To estimate the time needed to recover carbon removed and emitted during treatment, we compared the 7-year post-treatment carbon stocks for mechanical thinning and prescribed fire fuels reduction treatments in Sierra Nevada mixed-conifer forest and modeled annual carbon accumulation rates. Within our 7-year re-sample period, the burn only and understory thin treatments sequestered more carbon than had been removed or emitted during treatment The understory thin and burn, overstory thin, and overstory thin and burn continued to have net negative carbon stocks when emissions associated with treatment were subtracted from 7-year carbon stock gains. However, the size of the carbon deficit in the understory thin and burn 7 years post-treatment and the live tree growth rates suggest that the remaining trees may sequester treatment emissions within several more years of growth. Overstory tree thinning treatments resulted in a large carbon deficit and removed many of the largest trees that accumulate the most carbon annually, thereby increasing carbon stock recovery time. Our results indicate that while there is an initial carbon stock reduction associated with fuels treatments, treated forests can quickly recover carbon stocks if treatments do not remove large, fire-resistant overstory trees. (C) 2010 Elsevier B.V. All rights reserved. C1 [Hurteau, Matthew D.] No Arizona Univ, Natl Inst Climat Change Res, Flagstaff, AZ 86011 USA. [North, Malcolm] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95618 USA. RP Hurteau, MD (reprint author), No Arizona Univ, Natl Inst Climat Change Res, POB 6077, Flagstaff, AZ 86011 USA. EM Matthew.Hurteau@nau.edu RI Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 FU USDA Forest Service, Pacific Southwest Research Station; US Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University FX We thank J. Shields and C. Walters for collecting the 2008 data. S. Hurteau and two anonymous reviewers provided helpful feedback on earlier versions of this manuscript. This work was supported by the USDA Forest Service, Pacific Southwest Research Station and the US Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University. NR 57 TC 48 Z9 48 U1 1 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 30 PY 2010 VL 260 IS 5 BP 930 EP 937 DI 10.1016/j.foreco.2010.06.015 PG 8 WC Forestry SC Forestry GA 638AH UT WOS:000280861200038 ER PT J AU Boyd, JW Oldenburg, EW McMichael, GA AF Boyd, James W. Oldenburg, Eric W. McMichael, Geoffrey A. TI Color Photographic Index of Fall Chinook Salmon Embryonic Development and Accumulated Thermal Units SO PLOS ONE LA English DT Article ID TEMPERATURE; FISHES; TIME; EGGS AB Background: Knowledge of the relationship between accumulated thermal units and developmental stages of Chinook salmon embryos can be used to determine the approximate date of egg fertilization in natural redds, thus providing insight into oviposition timing of wild salmonids. However, few studies have documented time to different developmental stages of embryonic Chinook salmon and no reference color photographs are available. The objectives of this study were to construct an index relating developmental stages of hatchery-reared fall Chinook salmon embryos to time and temperature (e.g., degree days) and provide high-quality color photographs of each identified developmental stage. Methodology/Principal Findings: Fall Chinook salmon eggs were fertilized in a hatchery environment and sampled approximately every 72 h post-fertilization until 50% hatch. Known embryonic developmental features described for sockeye salmon were used to describe development of Chinook salmon embryos. A thermal sums model was used to describe the relationship between embryonic development rate and water temperature. Mean water temperature was 8.0 degrees C (range; 3.9-11.7 degrees C) during the study period. Nineteen stages of embryonic development were identified for fall Chinook salmon; two stages in the cleavage phase, one stage in the gastrulation phase, and sixteen stages in the organogenesis phase. The thermal sums model used in this study provided similar estimates of fall Chinook salmon embryonic development rate in water temperatures varying from 3.9-11.7 degrees C (mean = 8 degrees C) to those from several other studies rearing embryos in constant 8 degrees C water temperature. Conclusions/Significance: The developmental index provides a reasonable description of timing to known developmental stages of Chinook salmon embryos and was useful in determining developmental stages of wild fall Chinook salmon embryos excavated from redds in the Columbia River. This index should prove useful to other researchers who wish to approximate fertilization dates of Chinook salmon eggs from natural redds, assuming the thermal history of embryos is known. C1 [Boyd, James W.; Oldenburg, Eric W.; McMichael, Geoffrey A.] Ecol Grp, Richland, WA USA. RP Boyd, JW (reprint author), US Forest Serv, Eureka, MT USA. EM jboyd03@fs.fed.us FU Grant County Public Utility District #2, Washington FX Grant County Public Utility District #2, Washington funded this research (http://gcpud.org/). The funders had a role in study design, data collection and analysis, and decision to publish. NR 14 TC 2 Z9 2 U1 2 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 29 PY 2010 VL 5 IS 7 AR e11877 DI 10.1371/journal.pone.0011877 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 633QY UT WOS:000280520200039 PM 20686709 ER PT J AU Kang, J Badger, TM Ronis, MJJ Wu, XL AF Kang, Jie Badger, Thomas M. Ronis, Martin J. J. Wu, Xianli TI Non-isoflavone Phytochemicals in Soy and Their Health Effects SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Review DE Soy; soyasaponins; phytosterol; lignan; phytic acid; bioavailability; health effects ID GLYCINE-MAX MERRILL; BREAST-CANCER CELLS; BILIARY CHOLESTEROL SECRETION; HEXAPHOSPHATE INHIBITS GROWTH; ACID INOSITOL HEXAPHOSPHATE; PLASMA-CHOLESTEROL; SOYASAPOGENOL-A; PHYTIC ACID; IN-VITRO; PLANT STEROLS AB Epidemiological and clinical studies have linked consumption of soy foods with low incidences of a number of chronic diseases, such as cardiovascular diseases, cancer, and osteoporosis. Over the past decades, enormous research efforts have been made to identify bioactive components in soy. Isoflavones and soy protein have been suggested as the major bioactive components in soy and have received considerable attention. However, there are hundreds of phytochemical components in soybeans and soy-based foods. In recent years, accumulating evidence has suggested that the isoflavones or soy proteins stripped of phytochemicals only reflect certain aspects of health effects associated with soy consumption. Other phytochemicals, either alone or in combination with isoflavones or soy protein, may be involved in the health effects of soy. This review attempts to summarize major non-isoflavone phytochemicals in soy, as well as their bioavailability and health effects. In addition, a brief discussion of components formed during food processing is also included. C1 [Kang, Jie; Badger, Thomas M.; Ronis, Martin J. J.; Wu, Xianli] Univ Arkansas Med Sci, USDA, Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA. RP Wu, XL (reprint author), Univ Arkansas Med Sci, USDA, Arkansas Childrens Nutr Ctr, 15 Childrens Way, Little Rock, AR 72202 USA. EM wuxianli@uams.edu NR 194 TC 45 Z9 46 U1 4 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 28 PY 2010 VL 58 IS 14 BP 8119 EP 8133 DI 10.1021/jf100901b PG 15 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 627ED UT WOS:000280021100001 PM 20578706 ER PT J AU Joseph, JA Shukitt-Hale, B Brewer, GJ Weikel, KA Kalt, W Fisher, DR AF Joseph, James A. Shukitt-Hale, Barbara Brewer, Gregory J. Weikel, Karen A. Kalt, Wilhelmina Fisher, Derek R. TI Differential Protection among Fractionated Blueberry Polyphenolic Families against DA-, A beta(42)- and LPS-Induced Decrements in Ca2+ Buffering in Primary Hippocampal Cells SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Calcium recovery; blueberry fractions; anthocyanins; proanthocyanidins; reactive oxygen species ID LIPID-PEROXIDATION; OXIDATIVE STRESS; DIETARY SUPPLEMENTATION; CALCIUM HOMEOSTASIS; INHIBITORY-ACTION; COS-7 CELLS; RAT-LIVER; BRAIN; ANTIOXIDANT; FLAVONOIDS AB It has been postulated that at least part of the loss of cognitive function in aging may be the result of deficits in Ca2+ recovery (CAR) and increased oxidative/inflammatory (OX/INF) stress signaling. However, previous research showed that aged animals supplemented with blueberry (BB) extract showed fewer deficits in CAR, as well as motor and cognitive functional deficits. A recent subsequent experiment has shown that DA- or A beta(42)-induced deficits in CAR in primary hippocampal neuronal cells (HNC) were antagonized by BB extract, and (OX/INF) signaling was reduced. The present experiments assessed the most effective BB polyphenol fraction that could protect against OX/INF-induced deficits in CAR, ROS generation, or viability. HNCs treated with BB extract, BB fractions (e.g., proanthocyanidin, PAC), or control medium were exposed to dopamine (DA, 0.1 mM), amyloid beta (A beta(42), 25 mu M) or lipopolysaccharide (LPS, 1 mu g/mL). The results indicated that the degree of protection against deficits in CAR varied as a function of the stressor and was generally greater against A beta(42) and LPS than DA. The whole BB, anthocyanin (ANTH), and PRE-C18 fractions offered the greatest protection, whereas chlorogenic acid offered the lowest protection. Protective capabilities of the various fractions against ROS depended upon the stressor, where the BB extract and the combined PAC (high and low molecular weight) fraction offered the best protection against LPS and A beta(42) but were less effective against DA-induced ROS. The high and low molecular weight PACs and the ANTH fractions enhanced ROS production regardless of the stressor used, and this reflected increased activation of stress signals (e.g., P38 MAPK). The viability data indicated that the whole BB and combined PAC fraction showed greater protective effects against the stressors than the more fractionated polyphenolic components. Thus, these results suggest that, except for a few instances, the lesser the polyphenolic fractionation, the greater the effects, especially with respect to prevention of ROS and stress signal generation and viability. C1 [Brewer, Gregory J.] So Illinois Univ, Sch Med, Springfield, IL 62794 USA. [Kalt, Wilhelmina] Agr & Agri Food Canada, Kentville, NS B4N 1J5, Canada. [Joseph, James A.; Shukitt-Hale, Barbara; Weikel, Karen A.; Fisher, Derek R.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov FU NIA NIH HHS [R56 AG013435-10A2, R01 AG032431, R01 AG032431-02, R56 AG013435] NR 37 TC 23 Z9 27 U1 0 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 28 PY 2010 VL 58 IS 14 BP 8196 EP 8204 DI 10.1021/jf100144y PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 627ED UT WOS:000280021100012 PM 20597478 ER PT J AU Zhang, GH Luo, RT Cao, Y Shen, RC Zhang, XC AF Zhang, Guang-hui Luo, Rong-ting Cao, Ying Shen, Rui-chang Zhang, X. C. TI Correction factor to dye-measured flow velocity under varying water and sediment discharges SO JOURNAL OF HYDROLOGY LA English DT Article DE Overland flow; Velocity; Dye tracer; Correction factor; Sediment load ID SOIL-EROSION MODEL; OVERLAND-FLOW; SALTATING SEDIMENT; MEAN VELOCITY; SHALLOW FLOW; DETACHMENT; TRANSPORT; RESISTANCE AB Dye-tracing technique was a widely used method to determine the velocity of overland flow in soil erosion studies under both laboratory and field conditions. Few studies were performed to quantify the effects of sediment load on correction factor on steep slopes. This study was conducted to investigate the potential effects of sediment load on correction factor of overland flow to determine mean velocity in a glued hydraulic flume under a wide range of hydraulic conditions and sediment load. Slope gradient (S) varied from 8.7% to 34.2%, unit flow rate (q) from 0.66 to 5.26 x 10(-3) m(2) s(-1), and sediment load (Q(s)) from 0 to 6.95 kg m(-1) s(-1). The Reynolds number (Re) ranged from 350 to 5899. Mean velocity was calculated from the volumetric relation using measured flow depth by a digital level probe and flow discharge. The results showed that correction factor decreased as S increased and increased as Re increased for sediment-free flow with a mean value of 0.659. Correction factor could be estimated with a logarithmic function of S and Re (R(2) = 0.883). For the sediment-laden flow, correction factor varied from 0.233 to 0.682 with a mean value of 0.466. The correction factor decreased as sediment load (Q(s)) increased and increased as Re increased. It could be estimated with a logarithmic function of Re, and Q(s) (R(2) = 0.796). For the combination of sediment-free and sediment-laden flows, the correction factor varied from 0.233 to 0.783 with a mean of 0.505. The correction factor was inversely related to slope gradient and sediment load, and directly to Reynolds number (R(2) = 0.854). The overall results indicated that caution must be exercised and proper correction be made when using the dye tracer method to measure flow velocity in surface hydrology and erosion studies. Further studies with systematic advanced set up for sediment feeding are needed to quantify the potential effects of sediment load on correction factor under varying conditions of roughness element. (C) 2010 Elsevier B.V. All rights reserved. C1 [Zhang, Guang-hui] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Zhang, Guang-hui; Luo, Rong-ting; Cao, Ying; Shen, Rui-chang] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Zhang, X. C.] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. RP Zhang, GH (reprint author), Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. EM ghzhang@bnu.edu.cn RI li, dongsheng/B-2285-2012 FU National Key Basic Research Special Foundation [2007CB407204]; National Key Technologies RD Program [2006BA09B05] FX The experiments were conducted at the Fangshan Field Station of Beijing Normal University. Financial assistance for this work was provided by the National Key Basic Research Special Foundation Project (2007CB407204) and the National Key Technologies R&D Program (2006BA09B05). NR 26 TC 22 Z9 29 U1 4 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 J9 J HYDROL JI J. Hydrol. PD JUL 28 PY 2010 VL 389 IS 1-2 BP 205 EP 213 DI 10.1016/j.jhydrol.2010.05.050 PG 9 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 632TW UT WOS:000280452500018 ER PT J AU Li, XY Swaggerty, CL Kogut, MH Chiang, HI Wang, Y Genovese, KJ He, HQ Zhou, HJ AF Li, Xianyao Swaggerty, Christina L. Kogut, Michael H. Chiang, Hsin-I Wang, Ying Genovese, Kenneth J. He, Haiqi Zhou, Huaijun TI Gene Expression Profiling of the Local Cecal Response of Genetic Chicken Lines That Differ in Their Susceptibility to Campylobacter jejuni Colonization SO PLOS ONE LA English DT Article ID COMMERCIAL BROILER-CHICKENS; INNATE IMMUNE RESPONSIVENESS; PRO-INFLAMMATORY CYTOKINE; MESSENGER-RNA EXPRESSION; CIRCADIAN CLOCK; SALMONELLA INFECTION; AGILENT MICROARRAY; T-CELLS; HETEROPHILS; NUTRITION AB Campylobacter jejuni (C. jejuni) is one of the most common causes of human bacterial enteritis worldwide primarily due to contaminated poultry products. Previously, we found a significant difference in C. jejuni colonization in the ceca between two genetically distinct broiler lines (Line A (resistant) has less colony than line B (susceptible) on day 7 post inoculation). We hypothesize that different mechanisms between these two genetic lines may affect their ability to resist C. jejuni colonization in chickens. The molecular mechanisms of the local host response to C. jejuni colonization in chickens have not been well understood. In the present study, to profile the cecal gene expression in the response to C. jejuni colonization and to compare differences between two lines at the molecular level, RNA of ceca from two genetic lines of chickens (A and B) were applied to a chicken whole genome microarray for a pair-comparison between inoculated (I) and non-inoculated (N) chickens within each line and between lines. Our results demonstrated that metabolism process and insulin receptor signaling pathways are key contributors to the different response to C. jejuni colonization between lines A and B. With C. jejuni inoculation, lymphocyte activation and lymphoid organ development functions are important for line A host defenses, while cell differentiation, communication and signaling pathways are important for line B. Interestingly, circadian rhythm appears play a critical role in host response of the more resistant A line to C. jejuni colonization. A dramatic differential host response was observed between these two lines of chickens. The more susceptible line B chickens responded to C. jejuni inoculation with a dramatic up-regulation in lipid, glucose, and amino acid metabolism, which is undoubtedly for use in the response to the colonization with little or no change in immune host defenses. However, in more resistant line A birds the host defense responses were characterized by an up-regulation lymphocyte activation, probably by regulatory T cells and an increased expression of the NLR recognition receptor NALP1. To our knowledge, this is the first time each of these responses has been observed in the avian response to an intestinal bacterial pathogen. C1 [Li, Xianyao; Chiang, Hsin-I; Wang, Ying; Zhou, Huaijun] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. [Swaggerty, Christina L.; Kogut, Michael H.; Genovese, Kenneth J.; He, Haiqi] ARS, So Plains Agr Res Ctr, USDA, College Stn, TX USA. RP Li, XY (reprint author), Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. EM michael.kogut@ars.usda.gov; hjzhou@poultry.tamu.edu FU United States Department of Agriculture (USDA) [2007-35604-17903] FX This project was supported by National Research Initiative Grant no. 2007-35604-17903 from the United States Department of Agriculture (USDA) Cooperative State Research, Education, and Extension Service Animal Genome program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 29 Z9 32 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 28 PY 2010 VL 5 IS 7 AR e11827 DI 10.1371/journal.pone.0011827 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 633QX UT WOS:000280520100010 PM 20676366 ER PT J AU Kim, J Lee, JJ Kim, J Gardner, D Beachy, PA AF Kim, Jynho Lee, John J. Kim, James Gardner, Dale Beachy, Philip A. TI Arsenic antagonizes the Hedgehog pathway by preventing ciliary accumulation and reducing stability of the Gli2 transcriptional effector SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE arsenic trioxide; Hedgehog antagonist; primary cilium; smoothened; cancer ID BASAL-CELL CARCINOMA; SMALL-MOLECULE INHIBITORS; SIGNALING PATHWAY; PRIMARY CILIUM; MEDIATED TRANSCRIPTION; SMOOTHENED MUTATION; HUMAN HOMOLOG; STEM-CELLS; RAR-ALPHA; TRIOXIDE AB Aberrant Hedgehog (Hh) pathway activation has been implicated in cancers of diverse tissues and organs, and the tumor growth-inhibiting effects of pathway antagonists in animal models have stimulated efforts to develop pathway antagonists for human therapeutic purposes. These efforts have focused largely on cyclopamine derivatives or other compounds that mimic cyclopamine action in binding to and antagonizing Smoothened, a membrane transductory component. We report here that arsenicals, in contrast, antagonize the Hh pathway by targeting Gli transcriptional effectors; in the short term, arsenic blocks Hh-induced ciliary accumulation of Gli2, the primary activator of Hh-dependent transcription, and with prolonged incubation arsenic reduces steady-state levels of Gli2. Arsenicals active in Hh pathway antagonism include arsenic trioxide (ATO), a curative agent in clinical use for acute promyelocytic leukemia (APL); in our studies, ATO inhibited growth of Hh pathway-driven medulloblastoma allografts derived from Ptch(+/-)p53(-/-) mice within a range of serum levels comparable to those achieved in treatment of human APL. Arsenic thus could be tested rapidly as a therapeutic agent in malignant diseases associated with Hh pathway activation and could be particularly useful in such diseases that are inherently resistant or have acquired resistance to cyclopamine mimics. C1 [Kim, Jynho; Lee, John J.; Kim, James; Beachy, Philip A.] Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Dept Dev Biol, Stanford, CA 94305 USA. [Kim, Jynho; Lee, John J.; Kim, James; Beachy, Philip A.] Stanford Univ, Sch Med, Howard Hughes Med Inst, Stanford, CA 94305 USA. [Gardner, Dale] ARS, USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Beachy, PA (reprint author), Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Dept Dev Biol, Stanford, CA 94305 USA. EM pbeachy@stanford.edu FU Stanford Center for Children's Brain Tumors; National Institutes of Health FX We thank Dr. Thomas Moyer and the Metals Laboratory at the Mayo Clinic (Rochester, MN) for performing assays of serum arsenic levels and R. Toftgard (Karolinska Institutet) for Sufu-/- MEFs. This work was supported by a seed grant for pediatric brain tumor research from Stanford Center for Children's Brain Tumors and by funding from the National Institutes of Health. P. A. B. is an investigator of the Howard Hughes Medical Institute. NR 67 TC 129 Z9 133 U1 3 U2 15 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUL 27 PY 2010 VL 107 IS 30 BP 13432 EP 13437 DI 10.1073/pnas.1006822107 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 634RW UT WOS:000280602800045 PM 20624968 ER PT J AU Faris, JD Zhang, ZC Lu, HJ Lu, SW Reddy, L Cloutier, S Fellers, JP Meinhardt, SW Rasmussen, JB Xu, SS Oliver, RP Simons, KJ Friesen, TL AF Faris, Justin D. Zhang, Zengcui Lu, Huangjun Lu, Shunwen Reddy, Leela Cloutier, Sylvie Fellers, John P. Meinhardt, Steven W. Rasmussen, Jack B. Xu, Steven S. Oliver, Richard P. Simons, Kristin J. Friesen, Timothy L. TI A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE disease resistance; host-pathogen interaction; map-based cloning; necrotrophic fungus; Triticum ID PYRENOPHORA-TRITICI-REPENTIS; PTR TOXA; TSN1-TOXA INTERACTION; GENOMIC REGION; PROTEIN; EMERGENCE; BARLEY; TOXINS; LOCUS; SITE AB Plant disease resistance is often conferred by genes with nucleotide binding site (NBS) and leucine-rich repeat (LRR) or serine/threonine protein kinase (S/TPK) domains. Much less is known about mechanisms of susceptibility, particularly to necrotrophic fungal pathogens. The pathogens that cause the diseases tan spot and Stagonospora nodorum blotch on wheat produce effectors (host-selective toxins) that induce susceptibility in wheat lines harboring corresponding toxin sensitivity genes. The effector ToxA is produced by both pathogens, and sensitivity to ToxA is governed by the Tsn1 gene on wheat chromosome arm 5BL. Here, we report the cloning of Tsn1, which was found to have disease resistance gene-like features, including S/TPK and NBS-LRR domains. Mutagenesis revealed that all three domains are required for ToxA sensitivity, and hence disease susceptibility. Tsn1 is unique to ToxA-sensitive genotypes, and insensitive genotypes are null. Sequencing and phylogenetic analysis indicated that Tsn1 arose in the B-genome diploid progenitor of polyploid wheat through a gene-fusion event that gave rise to its unique structure. Although Tsn1 is necessary to mediate ToxA recognition, yeast two-hybrid experiments suggested that the Tsn1 protein does not interact directly with ToxA. Tsn1 transcription is tightly regulated by the circadian clock and light, providing further evidence that Tsn1-ToxA interactions are associated with photosynthesis pathways. This work suggests that these necrotrophic pathogens may thrive by subverting the resistance mechanisms acquired by plants to combat other pathogens. C1 [Faris, Justin D.; Lu, Shunwen; Xu, Steven S.; Simons, Kristin J.; Friesen, Timothy L.] ARS, Cereal Crops Res Unit, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. [Zhang, Zengcui; Lu, Huangjun; Meinhardt, Steven W.; Rasmussen, Jack B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Reddy, Leela] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58102 USA. [Cloutier, Sylvie] Agr & Agri Food Canada, Cereal Res Ctr, Winnipeg, MB R3T 2M9, Canada. [Fellers, John P.] Kansas State Univ, Plant Sci & Entomol Res Unit, Dept Plant Pathol, Throckmorton Plant Sci Ctr,USDA,ARS, Manhattan, KS 66506 USA. [Oliver, Richard P.] Murdoch Univ, Australian Ctr Necrotroph Fungal Pathogens, Murdoch, WA 6150, Australia. RP Faris, JD (reprint author), ARS, Cereal Crops Res Unit, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. EM justin.faris@ars.usda.gov FU US Department of Agriculture-Agricultural Research Service Current Research Information System (CRIS) [5442-22000-030-00D]; US Department of Agriculture National Institute of Food and Agriculture [2008-35301-19248] FX We thank Jamie Hauff, Rachel Lindgren, and Zhao-hui Liu for technical assistance and Xiaoyan Tang, Frank White, Piotr Gornicki, and Bikram Gill for critical reading of the manuscript. This research was supported by US Department of Agriculture-Agricultural Research Service Current Research Information System (CRIS) Project 5442-22000-030-00D and National Research Initiative Competitive Grant 2008-35301-19248 from the US Department of Agriculture National Institute of Food and Agriculture (to J.D.F.). NR 27 TC 129 Z9 131 U1 4 U2 40 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUL 27 PY 2010 VL 107 IS 30 BP 13544 EP 13549 DI 10.1073/pnas.1004090107 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 634RW UT WOS:000280602800064 PM 20624958 ER PT J AU Cingel, A Vinterhalter, B Vinterhalter, D Calic-Dragosavac, D Smigocki, A Ninkovic, S AF Cingel, Aleksandar Vinterhalter, Branka Vinterhalter, Dragan Calic-Dragosavac, Dusica Smigocki, Ann Ninkovic, Slavica TI Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica) SO AFRICAN JOURNAL OF BIOTECHNOLOGY LA English DT Article DE Agrobacterium tumefaciens; oryzacystatin; Solanum tuberosum L. ID CYSTEINE PROTEINASE-INHIBITOR; ORYZACYSTATIN-II; PLANT-REGENERATION; SHOOT REGENERATION; MOLECULAR-CLONING; DIPLOID POTATO; RICE SEEDS; EXPRESSION; TUMEFACIENS; RESISTANCE AB An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent. C1 [Cingel, Aleksandar; Vinterhalter, Branka; Vinterhalter, Dragan; Calic-Dragosavac, Dusica; Ninkovic, Slavica] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade 11060, Serbia. [Smigocki, Ann] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Ninkovic, S (reprint author), Univ Belgrade, Inst Biol Res Sinisa Stankovic, Despota Stefana 142, Belgrade 11060, Serbia. EM slavica@ibiss.bg.ac.rs FU Serbian Ministry of Science and Technology [143026B] FX This research was funded by the Serbian Ministry of Science and Technology through the grant No. 143026B. NR 38 TC 6 Z9 9 U1 0 U2 3 PU ACADEMIC JOURNALS PI VICTORIA ISLAND PA P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA SN 1684-5315 J9 AFR J BIOTECHNOL JI Afr. J. Biotechnol. PD JUL 26 PY 2010 VL 9 IS 30 BP 4644 EP 4650 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 646BU UT WOS:000281510900002 ER PT J AU Delhanty, PJD Sun, YX Visser, JA van Kerkwijk, A Huisman, M van IJcken, WFJ Swagemakers, S Smith, RG Themmen, APN van der Lely, AJ AF Delhanty, Patric J. D. Sun, Yuxiang Visser, Jenny A. van Kerkwijk, Anke Huisman, Martin van IJcken, Wilfred F. J. Swagemakers, Sigrid Smith, Roy G. Themmen, Axel P. N. van der Lely, Aart-Jan TI Unacylated Ghrelin Rapidly Modulates Lipogenic and Insulin Signaling Pathway Gene Expression in Metabolically Active Tissues of GHSR Deleted Mice SO PLOS ONE LA English DT Article ID HORMONE SECRETAGOGUE RECEPTOR; DES-ACYL GHRELIN; GLUCOSE-UPTAKE; ENDOTHELIAL LIPASE; LIPID-METABOLISM; DESACYL GHRELIN; SKELETAL-MUSCLE; ADIPOCYTES; SENSITIVITY; OBESITY AB Background: There is increasing evidence that unacylated ghrelin (UAG) improves insulin sensitivity and glucose homeostasis; however, the mechanism for this activity is not fully understood since a UAG receptor has not been discovered. Methodology/Principal Findings: To assess potential mechanisms of UAG action in vivo, we examined rapid effects of UAG on genome-wide expression patterns in fat, muscle and liver of growth hormone secretagogue receptor (GHSR)-ablated mice using microarrays. Expression data were analyzed using Ingenuity Pathways Analysis and Gene Set Enrichment Analysis. Regulation of subsets of these genes was verified by quantitative PCR in an independent experiment. UAG acutely regulated clusters of genes involved in glucose and lipid metabolism in all three tissues, consistent with enhancement of insulin sensitivity. Conclusions/Significance: Fat, muscle and liver are central to the control of lipid and glucose homeostasis. UAG rapidly modulates the expression of metabolically important genes in these tissues in GHSR-deleted mice indicating a direct, GHSR-independent, action of UAG to improve insulin sensitivity and metabolic profile. C1 [Delhanty, Patric J. D.; Visser, Jenny A.; van Kerkwijk, Anke; Huisman, Martin; Themmen, Axel P. N.; van der Lely, Aart-Jan] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [van IJcken, Wilfred F. J.] Erasmus MC, Ctr Biom, Rotterdam, Netherlands. [Swagemakers, Sigrid] Erasmus MC, Ctr Bioinformat, Rotterdam, Netherlands. [Swagemakers, Sigrid] Erasmus MC, Dept Genet, Rotterdam, Netherlands. [Sun, Yuxiang] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Smith, Roy G.] Scripps Res Inst Florida, Dept Metab & Aging, Jupiter, FL USA. RP Delhanty, PJD (reprint author), Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. EM p.delhanty@erasmusmc.nl RI Visser, Jenny /F-8156-2011; OI van IJcken, Wilfred/0000-0002-0421-8301 FU ZonMW, The Netherlands [91203022]; Alize Pharma SAS, Lyon, France FX Financial support for the study was provided by a Programme Grant from ZonMW, The Netherlands (#91203022) and Alize Pharma SAS, Lyon, France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 45 Z9 47 U1 1 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 26 PY 2010 VL 5 IS 7 AR e11749 DI 10.1371/journal.pone.0011749 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 630TN UT WOS:000280297300003 PM 20668691 ER PT J AU Rowland, LJ Ogden, EL Ehlenfeldt, MK AF Rowland, Lisa J. Ogden, Elizabeth L. Ehlenfeldt, Mark K. TI EST-PCR markers developed for highbush blueberry are also useful for genetic fingerprinting and relationship studies in rabbiteye blueberry SO SCIENTIA HORTICULTURAE LA English DT Article DE Expressed sequence tags; Vaccinium corymbosum; Vaccinium virgatum ID VACCINIUM-ASHEI READE; COLD-ACCLIMATION; DNA; CULTIVARS; SEQUENCE; RAPD; PLANTS AB The pedigrees of most rabbiteye blueberry (Vaccinium virgatum) cultivars can be traced back to four wild selections, 'Ethel', 'Clara', 'Myers', and 'Black Giant': thus, they result from a very narrow germplasm base and are highly related. Until now randomly amplified polymorphic DNA (RAPD) has been the only type of molecular marker used in rabbiteye blueberry. Here we have tested whether a type of sequence-tagged site (STS) marker which utilizes specific similar to 20-mer primers from expressed sequence tags (ESTs) of highbush blueberry (V. corymbosum), called EST-PCR markers, are useful for genetic fingerprinting and relationship studies in rabbiteye blueberry. Of 44 EST-PCR primer pairs, from an assortment of genes expressed in flower buds of cold acclimated and non-acclimated plants, and shown to amplify polymorphic fragments among a collection of highbush genotypes, 40(91%) resulted in successful amplification, and 33 of those (83%) amplified polymorphic fragments among the rabbiteye genotypes. The average number of scorable bands per primer pair was two. A dendrogram constructed from genetic similarity values, based on the EST-PCR marker data, tended to group siblings and parent/progeny together, generally agreeing with pedigree information. A group of 20 markers from five EST-PCR primer pairs distinguished all the genotypes in this study. These markers are as easy to generate and as affordable as RAPDs, but are based on actual gene sequences, and should have general utility for DNA fingerprinting, genetic diversity, and mapping studies. Published by Elsevier B.V. C1 [Rowland, Lisa J.; Ogden, Elizabeth L.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Ehlenfeldt, Mark K.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Blueberry & Cranberry Res Ctr, Chatsworth, NJ 08019 USA. RP Rowland, LJ (reprint author), ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Genet Improvement Fruits & Vegetables Lab, Bldg 010A,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Jeannine.Rowland@ars.usda.gov NR 27 TC 8 Z9 10 U1 0 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUL 26 PY 2010 VL 125 IS 4 BP 779 EP 784 DI 10.1016/j.scienta.2010.05.008 PG 6 WC Horticulture SC Agriculture GA 635MO UT WOS:000280660000036 ER PT J AU Krueger, WK Gutierrez-Banuelos, H Carstens, GE Min, BR Pinchak, WE Gomez, RR Anderson, RC Krueger, NA Forbes, TDA AF Krueger, W. K. Gutierrez-Banuelos, H. Carstens, G. E. Min, B. R. Pinchak, W. E. Gomez, R. R. Anderson, R. C. Krueger, N. A. Forbes, T. D. A. TI Effects of dietary tannin source on performance, feed efficiency, ruminal fermentation, and carcass and non-carcass traits in steers fed a high-grain diet SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Average daily gain; Beef cattle; Condensed tannin; Hydrolysable tannin; Methane; Volatile fatty acid ID CONDENSED TANNINS; IN-VITRO; METHANE EMISSIONS; CATTLE; SHEEP; EXTRACT; SORGHUM; SUPPLEMENTATION; NUTRITION; GROWTH AB The objective of this study, which was part of an integrated project to investigate the antimicrobial effects of dietary tannins on native food borne pathogens in beef cattle, was to examine the effects of source of tannin (condensed, CT, vs. hydrolysable, HT) on performance, feed efficiency, ruminal fermentation parameters, and carcass and non-carcass traits in finishing beef steers. Thirty-six crossbred steers averaging 414 +/- 40 kg BW were stratified by initial BW and randomly assigned to one of three treatments: control (CN), CT, or HT tannins. Commercially available tannin extracts were added to a high-grain diet (ME = 11.9 MJ/kg DM) at 14.9 g/kg DM. Mimosa and chestnut extracts provided condensed tannin and hydrolysable tannin, respectively. Steers were individually fed using Calan gate feeders a high-grain diet. Rumen fluid was collected on days 0, 21, and 42 via stomach tube and analyzed for VFA and in vitro methane producing activity. Cattle were harvested at the end of the study and carcass data collected 24-h postharvest. There was no effect (P>0.05) of tannin supplementation on animal performance, ruminal fermentation parameters, in vitro methane producing activity, or carcass and non-carcass traits, except for HCW, EBW, and rumen mass and empty GIT (g/kg EBW). Condensed tannin steers had 3.7% lower (P<0.05) HCW compared to CN with HT steers having intermediate HOW. Hydrolysable tannin treated steers had 2.8% lower (P<0.05) EBW compared to CN while CT steers had intermediate EBW: CT treated steers also had 15.2% higher (P<0.05) rumen mass (g/kg EBW) compared to HT with CN steers being intermediate. This resulted in a 10.2% increase (P<0.05) in total empty GIT (g/kg EBW) for HT steers compared to CT steers with CN steers bring intermediate. There was a treatment x day interaction for butyrate concentration. For steers fed CT, there was a linear increase in butyrate while the HT steers remained relatively stable and the control steers had numerically lower butyrate. Despite the significant interaction, treatment means on day 42 were not significantly different. Results indicate that neither source of dietary tannin affected performance and feed efficiency. There were no detrimental effects of tannins on other offal measured indicating that tannins supplementation may be a viable option in finishing beef cattle if bactericidal efficacy is established. More research is needed to further our understanding of how tannin supplementation may fit into real-life feedlot situations. (C) 2010 Elsevier B.V. All rights reserved. C1 [Krueger, W. K.; Carstens, G. E.] Texas A&M Univ, Intercollegiate Fac Nutr, College Stn, TX 77843 USA. [Krueger, W. K.; Carstens, G. E.; Gomez, R. R.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Anderson, R. C.; Krueger, N. A.] ARS, USDA, Food & Feed Safety Res Unit, College Stn, TX 77843 USA. [Min, B. R.; Pinchak, W. E.] Texas Agrilife Res, Vernon, TX 76384 USA. [Forbes, T. D. A.] Texas Agrilife Res, Uvalde, TX 78801 USA. RP Pinchak, WE (reprint author), 11708 Hwy 70 S,POB 1658, Vernon, TX 76385 USA. EM w-pinchak@tamu.edu FU Cattlemen's Beef Board; state beef councils by the National Cattlemen's Beef Association FX This project was funded (or funded in part), by beef and veal producers and importers through their $1-per-head checkoff and was produced for the Cattlemen's Beef Board and state beef councils by the National Cattlemen's Beef Association. NR 44 TC 32 Z9 33 U1 0 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD JUL 20 PY 2010 VL 159 IS 1-2 BP 1 EP 9 DI 10.1016/j.anifeedsci.2010.05.003 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 631YT UT WOS:000280387400001 ER PT J AU Kadota, T Takezawa, Y Hirano, S Tajima, O Maragos, CM Nakajima, T Tanaka, T Kamata, Y Sugita-Konishi, Y AF Kadota, Tomoyuki Takezawa, Yoko Hirano, Satoshi Tajima, Osamu Maragos, Chris M. Nakajima, Takashi Tanaka, Toshitsugu Kamata, Yoichi Sugita-Konishi, Yoshiko TI Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay SO ANALYTICA CHIMICA ACTA LA English DT Article DE Mycotoxin; Surface plasmon resonance; Immunoassay ID TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; FUSARIUM MYCOTOXINS; TRICHOTHECENE MYCOTOXINS; MONOCLONAL-ANTIBODIES; MASKED DEOXYNIVALENOL; NATURAL OCCURRENCE; ZEARALENONE; BARLEY; CEREALS AB A surface plasmon resonance (SPR) immunoassay using a monoclonal antibody was developed to measure nivalenol (NIV) and deoxynivalenol (DON) contamination in wheat. A highly sensitive and stable DON-immobilized sensor chip was prepared, and an SPR detection procedure was developed. The competitive inhibition assay used a monoclonal antibody that cross-reacts with NIV and DON. The half maximal inhibitory concentration (IC(50)) values of the SPR assay were 28.8 and 14.9 ng mL(-1) for NIV and DON, respectively. The combined responses of NIV and DON in wheat were obtained using a simultaneous detection assay in a one-step cleanup procedure. NIV and DON were separated using a commercial DON-specific immunoaffinity column (IAC) and their responses were obtained using an independent detection assay. Spiked tests using these toxins revealed that recoveries were in the range 91.5-107% with good relative standard deviations (RSDs) (0.40-4.1%) and that detection limits were 0.1 and 0.05 mg kg(-1) for NIV and DON, respectively. The independent detection using IAC showed detection limits of 0.2 and 0.1 mg kg(-1) for NIV and DON, respectively. SPR analysis results were correlated with those obtained using a conventional LC/MS/MS method for wheat co-contaminated with NIV and DON. These results suggested that the developed SPR assay is a practical method to rapidly screen the NIV and DON co-contamination of wheat and one of a very few immunoassays to detect NIV directly. (c) 2010 Elsevier B.V. All rights reserved. C1 [Kadota, Tomoyuki; Takezawa, Yoko; Hirano, Satoshi; Tajima, Osamu] Kirin Holdings Co Ltd, Ctr Food Safety Sci, Cent Labs Frontier Technol, Gunma 3701295, Japan. [Kadota, Tomoyuki] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan. [Maragos, Chris M.] USDA ARS, Mycotoxin Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Nakajima, Takashi] Natl Agr Res Ctr Kyushu Okinawa Reg, Res Team Fusarium Head Blight Control, Kumamoto 8611192, Japan. [Tanaka, Toshitsugu] Kobe Inst Hlth, Chuo Ku, Kobe, Hyogo 6500046, Japan. [Kamata, Yoichi; Sugita-Konishi, Yoshiko] Natl Inst Hlth Sci, Div Microbiol, Setagaya Ku, Tokyo 1588501, Japan. RP Kadota, T (reprint author), Kirin Holdings Co Ltd, Ctr Food Safety Sci, Cent Labs Frontier Technol, 3 Miyahara, Gunma 3701295, Japan. EM Tomoyuki_Kadota@kirin.co.jp FU Kirin Beverage Company, Ltd.; Kirin Holdings Company, Ltd. FX The authors thank Mr. Yuji Ito of Kirin Beverage Company, Ltd., and Mr. Yuji Sakamoto of Kirin Holdings Company, Ltd., for supporting this work. NR 36 TC 32 Z9 33 U1 3 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0003-2670 J9 ANAL CHIM ACTA JI Anal. Chim. Acta PD JUL 19 PY 2010 VL 673 IS 2 BP 173 EP 178 DI 10.1016/j.aca.2010.05.028 PG 6 WC Chemistry, Analytical SC Chemistry GA 629PZ UT WOS:000280213500010 PM 20599032 ER PT J AU Rodriguez-Valle, M Lew-Tabor, A Gondro, C Moolhuijzen, P Vance, M Guerrero, FD Bellgard, M Jorgensen, W AF Rodriguez-Valle, Manuel Lew-Tabor, Ala Gondro, Cedric Moolhuijzen, Paula Vance, Megan Guerrero, Felix D. Bellgard, Matthew Jorgensen, Wayne TI Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle SO BMC GENOMICS LA English DT Article ID SERINE PROTEINASE-INHIBITORS; IMMUNOGLOBULIN-BINDING PROTEINS; TICK IXODES-SCAPULARIS; SALIVARY-GLAND; HAEMAPHYSALIS-LONGICORNIS; HARD TICK; BIOCHEMICAL-CHARACTERIZATION; STRUCTURAL-CHARACTERIZATION; MOLECULAR CHARACTERIZATION; BRAZILIAN POPULATION AB Background: Rhipicephalus (Boophilus) microplus is an obligate blood feeder which is host specific to cattle. Existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during the tick - host interaction is poor. Results: Global analysis of gene expression changes in whole R. microplus ticks during larval, pre-attachment and early adult stages feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. Among the 13,601 R. microplus transcripts from BmiGI Version 2 we identified 297 high and 17 low expressed transcripts that were significantly differentially expressed between R. microplus feeding on tick resistant cattle [Bos indicus (Brahman)] compared to R. microplus feeding on tick susceptible cattle [Bos taurus (Holstein-Friesian)] (p <= 0.001). These include genes encoding enzymes involved in primary metabolism, and genes related to stress, defence, cell wall modification, cellular signaling, receptor, and cuticle formation. Microarrays were validated by qRT-PCR analysis of selected transcripts using three housekeeping genes as normalization controls. Conclusion: The analysis of all tick stages under survey suggested a coordinated regulation of defence proteins, proteases and protease inhibitors to achieve successful attachment and survival of R. microplus on different host breeds, particularly Bos indicus cattle. R. microplus ticks demonstrate different transcript expression patterns when they encounter tick resistant and susceptible breeds of cattle. In this study we provide the first transcriptome evidence demonstrating the influence of tick resistant and susceptible cattle breeds on transcript expression patterns and the molecular physiology of ticks during host attachment and feeding. The microarray data used in this analysis have been submitted to NCBI GEO database under accession number GSE20605 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE20605. C1 [Rodriguez-Valle, Manuel; Lew-Tabor, Ala; Gondro, Cedric; Moolhuijzen, Paula; Vance, Megan; Jorgensen, Wayne] Cooperat Res Ctr Beef Genet Technol, Armidale, NSW, Australia. [Rodriguez-Valle, Manuel; Lew-Tabor, Ala; Vance, Megan; Jorgensen, Wayne] Queensland Primary Ind & Fisheries, Ind Serv, Dept Employment Econ Dev & Innovat, Yeerongpilly, Qld 4105, Australia. [Lew-Tabor, Ala; Moolhuijzen, Paula; Bellgard, Matthew] Murdoch Univ, Ctr Comparat Gen, Perth, WA 6150, Australia. [Guerrero, Felix D.] USDA ARS, Knipling Bushland US Livestock Insect Res Lab, Kerrville, TX 78028 USA. [Gondro, Cedric] Univ New England, TIGB Inst Genet & Bioinformat, Armidale, NSW 2351, Australia. RP Rodriguez-Valle, M (reprint author), Cooperat Res Ctr Beef Genet Technol, Armidale, NSW, Australia. EM manuel.rodriguezvalle@deedi.qld.gov.au RI Rodriguez-Valle, Manuel/A-5272-2010; Lew-Tabor, Ala/A-5286-2010; gondro, cedric/B-4362-2010; OI Rodriguez-Valle, Manuel/0000-0003-2304-8085; Lew-Tabor, Ala/0000-0001-7147-1078; gondro, cedric/0000-0003-0666-656X; Vance, Megan/0000-0001-5824-1008 FU CRC; Queensland Smart State Innovation Fund; Meat & Livestock Australia [BP-NBP-0501] FX This work was supported by the CRC for Beef Genetic Technologies and Queensland Smart State Innovation Fund (National and International Research Alliances Program). The small cattle trial (under The University of Queensland Animal Ethics Committee Approval No. SVS/872/07/CRC) was supported by Meat & Livestock Australia grant BP-NBP-0501. We are grateful to Dr Louise Jackson (QPI&F), Emily Piper and Prof Nick Johnson (UQ) for their assistance with Animal Ethics approval, tick collection and cattle management. We also thank Dr Louise Jackson (Biosecurity, QPI&F, Yeerongpilly, Qld, Australia) for the provision of N strain ticks to undertake this study. Thanks to Bev Sullivan (IPAA) for the grammar revision of this paper. NR 100 TC 20 Z9 20 U1 1 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 19 PY 2010 VL 11 AR 437 DI 10.1186/1471-2164-11-437 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 662DJ UT WOS:000282787100001 PM 20637126 ER PT J AU Lawson, S Lunney, J Zuckermann, F Osorio, F Nelson, E Welbon, C Clement, T Fang, Y Wong, S Kulas, K Christopher-Hennings, J AF Lawson, Steven Lunney, Joan Zuckermann, Federico Osorio, Fernando Nelson, Eric Welbon, Craig Clement, Travis Fang, Ying Wong, Susan Kulas, Karen Christopher-Hennings, Jane TI Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: Assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination SO VACCINE LA English DT Article DE PRRSV; Luminex; Swine cytokines ID PROINFLAMMATORY CYTOKINES; SEROLOGIC RESPONSE; INFECTION; PROTECTION; CHALLENGE; IL-12; PIGS; INTERLEUKIN-12; IMMUNOASSAYS; VIRULENT AB A Luminex (Luminex Corp., Austin, TX) multiplex swine cytokine assay was developed to measure 8 cytokines simultaneously in pig serum for use in assessment of vaccine candidates. The fluorescent microsphere immunoassay (FMIA) was tested on archived sera in a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine/challenge study. This FMIA simultaneously detects innate (IL-1 beta, IL-8, IFN-alpha, TNF-alpha, IL-12), regulatory (IL-10), Th1 (IFN-gamma) and Th2 (IL-4) cytokines. These proteins were measured to evaluate serum cytokine levels associated with vaccination strategies that provided for different levels of protective immunity against PRRSV. Pigs were vaccinated with a modified-live virus (MLV) vaccine and subsequently challenged with a non-identical PRRSV isolate (93% identity in the glycoprotein (GP5) gene). Protection (as defined by no serum viremia) was observed in the MLV vaccinated pigs after PRRSV challenge but not those vaccinated with killed virus vaccine with adjuvant (KV/ADJ) (99% identity in the GP5 gene to the challenge strain) or non-vaccinates. Significantly elevated levels of IL-12 were observed in the KV/ADJ group compared to MLV vaccinated and control groups. However, this significant increase in serum IL-12 did not correlate with protection against PRRSV viremia. Additional studies using this assay to measure the local cytokine tissue responses may help in defining a protective cytokine response and would be useful for the targeted design of efficacious vaccines, not only for PRRSV, but also for other swine pathogens. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Lawson, Steven; Nelson, Eric; Welbon, Craig; Clement, Travis; Fang, Ying; Christopher-Hennings, Jane] S Dakota State Univ, Dept Vet Sci, Brookings, SD 57007 USA. [Wong, Susan; Kulas, Karen] Wadsworth Ctr NYSDOH, Diagnost Immunol Lab, Albany, NY 12201 USA. [Osorio, Fernando] Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA. [Zuckermann, Federico] Univ Illinois, Coll Vet Med, Dept Pathobiol, Champaign, IL 61802 USA. [Lunney, Joan] ARS, Anim Parasit Dis Lab, ANRI, USDA, Beltsville, MD 20705 USA. RP Christopher-Hennings, J (reprint author), S Dakota State Univ, Dept Vet Sci, N Campus Dr, Brookings, SD 57007 USA. EM Jane.Hennings@sdstate.edu FU National Pork Board [08-189] FX This work was funded through the National Pork Board (#08-189). NR 32 TC 27 Z9 30 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD JUL 19 PY 2010 VL 28 IS 32 BP 5356 EP 5364 DI 10.1016/j.vaccine.2010.05.016 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 639QF UT WOS:000280988600030 PM 20488263 ER PT J AU Wang, TJ Zhang, F Richards, JB Kestenbaum, B van Meurs, JB Berry, D Kiel, DP Streeten, EA Ohlsson, C Koller, DL Peltonen, L Cooper, JD O'Reilly, PF Houston, DK Glazer, NL Vandenput, L Peacock, M Shi, J Rivadeneira, F McCarthy, MI Anneli, P de Boer, IH Mangino, M Kato, B Smyth, DJ Booth, SL Jacques, PF Burke, GL Goodarzi, M Cheung, CL Wolf, M Rice, K Goltzman, D Hidiroglou, N Ladouceur, M Wareham, NJ Hocking, LJ Hart, D Arden, NK Cooper, C Malik, S Fraser, WD Hartikainen, AL Zhai, GJ Macdonald, HM Forouhi, NG Loos, RJF Reid, DM Hakim, A Dennison, E Liu, YM Power, C Stevens, HE Jaana, L Vasan, RS Soranzo, N Bojunga, J Psaty, BM Lorentzon, M Foroud, T Harris, TB Hofman, A Jonsson, JO Cauley, JA Uitterlinden, AG Gibson, Q Jarvelin, MR Karasik, D Siscovick, DS Econs, MJ Kritchevsky, SB Florez, JC Todd, JA Dupuis, J Hypponen, E Spector, TD AF Wang, Thomas J. Zhang, Feng Richards, J. Brent Kestenbaum, Bryan van Meurs, Joyce B. Berry, Diane Kiel, Douglas P. Streeten, Elizabeth A. Ohlsson, Claes Koller, Daniel L. Peltonen, Leena Cooper, Jason D. O'Reilly, Paul F. Houston, Denise K. Glazer, Nicole L. Vandenput, Liesbeth Peacock, Munro Shi, Julia Rivadeneira, Fernando McCarthy, Mark I. Anneli, Pouta de Boer, Ian H. Mangino, Massimo Kato, Bernet Smyth, Deborah J. Booth, Sarah L. Jacques, Paul F. Burke, Greg L. Goodarzi, Mark Cheung, Ching-Lung Wolf, Myles Rice, Kenneth Goltzman, David Hidiroglou, Nick Ladouceur, Martin Wareham, Nicholas J. Hocking, Lynne J. Hart, Deborah Arden, Nigel K. Cooper, Cyrus Malik, Suneil Fraser, William D. Hartikainen, Anna-Liisa Zhai, Guangju Macdonald, Helen M. Forouhi, Nita G. Loos, Ruth J. F. Reid, David M. Hakim, Alan Dennison, Elaine Liu, Yongmei Power, Chris Stevens, Helen E. Jaana, Laitinen Vasan, Ramachandran S. Soranzo, Nicole Bojunga, Joerg Psaty, Bruce M. Lorentzon, Mattias Foroud, Tatiana Harris, Tamara B. Hofman, Albert Jonsson, John-Olov Cauley, Jane A. Uitterlinden, Andre G. Gibson, Quince Jarvelin, Marjo-Riitta Karasik, David Siscovick, David S. Econs, Michael J. Kritchevsky, Stephen B. Florez, Jose C. Todd, John A. Dupuis, Josee Hyppoenen, Elina Spector, Timothy D. TI Common genetic determinants of vitamin D insufficiency: a genome-wide association study SO LANCET LA English DT Article ID D-BINDING PROTEIN; RANDOMIZED CONTROLLED-TRIALS; LEMLI-OPITZ-SYNDROME; D DEFICIENCY; SERUM 25-HYDROXYVITAMIN-D; PLASMA-CONCENTRATIONS; D SUPPLEMENTATION; CANCER RISK; METAANALYSIS; CALCIUM AB Background Vitamin D is crucial for maintenance of musculoskeletal health, and might also have a role in extraskeletal tissues. Determinants of circulating 25-hydroxyvitamin D concentrations include sun exposure and diet, but high heritability suggests that genetic factors could also play a part. We aimed to identify common genetic variants affecting vitamin D concentrations and risk of insufficiency. Methods We undertook a genome-wide association study of 25-hydroxyvitamin D concentrations in 33 996 individuals of European descent from 15 cohorts. Five epidemiological cohorts were designated as discovery cohorts (n=16 125), five as in-silico replication cohorts (n=9367), and five as de-novo replication cohorts (n=8504). 25-hydroxyvitamin D concentrations were measured by radioimmunoassay, chemiluminescent assay, ELISA, or mass spectrometry. Vitamin D insufficiency was defined as concentrations lower than 75 nmol/L or 50 nmol/L. We combined results of genome-wide analyses across cohorts using Z-score-weighted meta-analysis. Genotype scores were constructed for confirmed variants. Findings Variants at three loci reached genome-wide significance in discovery cohorts for association with 25-hydroxyvitamin D concentrations, and were confirmed in replication cohorts: 4p12 (overall p=1.9x10(-109) for rs2282679, in GC); 11q12 (p=2.1x10(-22) for rs12785878, near DHCR7); and 11p15 (p=3.3x10(-20) for rs10741657, near CYP2R1). Variants at an additional locus (20q13, CYP24A1) were genome-wide significant in the pooled sample (p=6.0x10(-10) for rs6013897). Participants with a genotype score (combining the three confirmed variants) in the highest quartile were at increased risk of having 25-hydroxyvitamin D concentrations lower than 75 nmol/L (OR 2.47, 95% CI 2.20-2.78, p=2.3x10(-48)) or lower than 50 nmol/L (1.92, 1.70-2-16, p=1.0x10(-26)) compared with those in the lowest quartile. Interpretation Variants near genes involved in cholesterol synthesis, hydroxylation, and vitamin D transport affect vitamin D status. Genetic variation at these loci identifies individuals who have substantially raised risk of vitamin D insufficiency. C1 [Wang, Thomas J.] Massachusetts Gen Hosp, Dept Med, Div Cardiol, Boston, MA 02114 USA. [Florez, Jose C.] Massachusetts Gen Hosp, Diabet Res Ctr, Diabet Unit, Boston, MA 02114 USA. [Florez, Jose C.] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. [Kiel, Douglas P.; Cheung, Ching-Lung; Karasik, David] Hebrew SeniorLife, Inst Aging Res, Genet Epidemiol Program, Boston, MA USA. [Wang, Thomas J.; Florez, Jose C.] Harvard Univ, Sch Med, Boston, MA USA. [Wang, Thomas J.; Kiel, Douglas P.; Vasan, Ramachandran S.; Dupuis, Josee] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Zhang, Feng; Mangino, Massimo; Kato, Bernet; Hart, Deborah; Zhai, Guangju; Soranzo, Nicole; Spector, Timothy D.] Kings Coll London, Dept Twin Res & Genet Epidemiol, London SE1 7EH, England. [Richards, J. Brent; Ladouceur, Martin] McGill Univ, Jewish Gen Hosp, Montreal, PQ H3T 1E2, Canada. [Goltzman, David] McGill Univ, Ctr Hlth, Montreal, PQ H3T 1E2, Canada. [Richards, J. Brent; Goltzman, David; Ladouceur, Martin] McGill Univ, Dept Med, Montreal, PQ H3T 1E2, Canada. [Richards, J. Brent; Ladouceur, Martin] McGill Univ, Dept Human Genet, Montreal, PQ H3T 1E2, Canada. [Richards, J. Brent; Ladouceur, Martin] McGill Univ, Dept Epidemiol & Biostat, Montreal, PQ H3T 1E2, Canada. [Kestenbaum, Bryan; de Boer, Ian H.] Univ Washington, Harborview Med Ctr, Kidney Res Inst, Div Nephrol, Seattle, WA 98104 USA. [Glazer, Nicole L.; Rice, Kenneth; Psaty, Bruce M.; Siscovick, David S.] Univ Washington, Dept Med, Seattle, WA 98104 USA. [Glazer, Nicole L.; Rice, Kenneth; Siscovick, David S.] Univ Washington, Cardiovasc Hlth Res Unit, Seattle, WA 98104 USA. [Psaty, Bruce M.; Siscovick, David S.] Univ Washington, Dept Epidemiol, Seattle, WA 98104 USA. [Psaty, Bruce M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98104 USA. [van Meurs, Joyce B.; Rivadeneira, Fernando; Uitterlinden, Andre G.; Gibson, Quince] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Hofman, Albert; Uitterlinden, Andre G.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Uitterlinden, Andre G.] Erasmus MC, Dept Klin Genet, Rotterdam, Netherlands. [van Meurs, Joyce B.; Rivadeneira, Fernando; Hofman, Albert; Uitterlinden, Andre G.] NGI, Sponsored NCHA, Rotterdam, Netherlands. [Berry, Diane; Power, Chris; Hyppoenen, Elina] UCL, Inst Child Hlth, MRC Ctr Epidemiol Child Hlth, London, England. [Berry, Diane; Power, Chris; Hyppoenen, Elina] Ctr Paediat Epidemiol & Biostat, London, England. [Streeten, Elizabeth A.; Shi, Julia] Univ Maryland, Sch Med, Div Endocrinol, Baltimore, MD 21201 USA. [Ohlsson, Claes; Vandenput, Liesbeth; Lorentzon, Mattias] Univ Gothenburg, Inst Med, Dept Internal Med, Sahlgrenska Acad, Gothenburg, Sweden. [Jonsson, John-Olov] Univ Gothenburg, Sahlgrenska Acad, Inst Neurosci & Physiol, Dept Physiol, Gothenburg, Sweden. [Koller, Daniel L.; Peacock, Munro; Foroud, Tatiana; Econs, Michael J.] Indiana Univ, Sch Med, Indianapolis, IN USA. [Peltonen, Leena; Soranzo, Nicole] Wellcome Trust Sanger Inst, Hinxton, England. [Peltonen, Leena] Univ Helsinki, Helsinki, Finland. [Peltonen, Leena] Inst Mol Med Finland, Natl Inst Hlth & Welf, Helsinki, Finland. [Cooper, Jason D.; Smyth, Deborah J.; Stevens, Helen E.; Todd, John A.] Univ Cambridge, JDRF WT Diabet & Inflammat Lab, Cambridge, England. [O'Reilly, Paul F.; Jarvelin, Marjo-Riitta] Univ London Imperial Coll Sci Technol & Med, Dept Epidemiol & Publ Hlth, Fac Med, London, England. [Houston, Denise K.; Liu, Yongmei; Kritchevsky, Stephen B.] Wake Forest Univ, Sch Med, Sticht Ctr Aging, Winston Salem, NC 27109 USA. [Burke, Greg L.] Wake Forest Univ, Sch Med, Div Publ Hlth Sci, Winston Salem, NC 27109 USA. [McCarthy, Mark I.] Univ Oxford, OCDEM, Oxford, England. [McCarthy, Mark I.] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford, England. [Arden, Nigel K.; Cooper, Cyrus] Univ Oxford, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. [McCarthy, Mark I.] Churchill Hosp, Oxford NIHR Biomed Res Ctr, Oxford OX3 7LJ, England. [Anneli, Pouta; Jarvelin, Marjo-Riitta] Natl Inst Hlth & Welf, Oulu, Finland. [Booth, Sarah L.; Jacques, Paul F.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Boston, MA 02111 USA. [Goodarzi, Mark] Cedars Sinai Med Ctr, Dept Med, Los Angeles, CA 90048 USA. [Wolf, Myles] Univ Miami, Miller Sch Med, Div Nephrol & Hypertens, Miami, FL 33136 USA. [Cheung, Ching-Lung] ASTAR, Genome Inst Singapore Computat & Math Biol, Singapore, Singapore. [Hidiroglou, Nick] Hlth Canada, Ottawa, ON K1A 0L2, Canada. [Wareham, Nicholas J.; Forouhi, Nita G.; Loos, Ruth J. F.] Addenbrookes Hosp, MRC Epidemiol Unit, Inst Metab Sci, Cambridge, England. [Hocking, Lynne J.; Macdonald, Helen M.; Reid, David M.] Univ Aberdeen, Div Appl Med, Bone & Musculoskeletal Res Programme, Aberdeen, Scotland. [Arden, Nigel K.; Cooper, Cyrus; Dennison, Elaine] Univ Southampton, MRC Epidemiol Resource Ctr, Southampton, Hants, England. [Malik, Suneil] Publ Hlth Agcy Canada, Off Biotechnol Genom & Populat Hlth, Toronto, ON, Canada. [Fraser, William D.] Univ Liverpool, Unit Clin Chem, Sch Clin Sci, Liverpool L69 3BX, Merseyside, England. [Hartikainen, Anna-Liisa] Univ Oulu, Dept Obstet & Gynaecol, Oulu, Finland. [Jaana, Laitinen; Jarvelin, Marjo-Riitta] Univ Oulu, Inst Hlth Sci, Oulu, Finland. [Jarvelin, Marjo-Riitta] Univ Oulu, Bioctr Oulu, Oulu, Finland. [Hakim, Alan] Whipps Cross Univ Hosp, Dept Rheumatol, London, England. [Jaana, Laitinen] Finnish Inst Occupat Hlth, Oulu, Finland. [Vasan, Ramachandran S.] Boston Univ, Sch Med, Div Prevent Med, Boston, MA 02118 USA. [Dupuis, Josee] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA. [Bojunga, Joerg] Klinikum Johann Wolfgang Goethe Univ, Frankfurt, Germany. [Harris, Tamara B.] NIA, NIH, Bethesda, MD 20892 USA. [Cauley, Jane A.] Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USA. [Florez, Jose C.] Broad Inst, Program Med & Populat Genet, Cambridge, MA USA. RP Spector, TD (reprint author), Kings Coll London, Dept Twin Res & Genet Epidemiol, St Thomas Hosp Campus,1st Floor S Wing Block 4,We, London SE1 7EH, England. EM tjwang@partners.org; e.hypponen@ich.ucl.ac.uk; tim.spector@kcl.ac.uk RI CHEUNG, Ching-Lung/C-2529-2012; Spector, Tim/F-6533-2012; Rice, Kenneth/A-4150-2013; Hypponen, Elina/B-2596-2014; Rivadeneira, Fernando/O-5385-2015; Osborne, Nicholas/N-4915-2015; mangino, massimo/F-5134-2011; OI Dupuis, Josee/0000-0003-2871-3603; Kritchevsky, Stephen/0000-0003-3336-6781; Kiel, Douglas/0000-0001-8474-0310; /0000-0002-6233-9144; Karasik, David/0000-0002-8826-0530; Forouhi, Nita/0000-0002-5041-248X; Jarvelin, Marjo-Riitta/0000-0002-2149-0630; Vandenput, Liesbeth/0000-0002-1712-6131; Cauley, Jane A/0000-0003-0752-4408; Rice, Kenneth/0000-0001-5779-4495; Hypponen, Elina/0000-0003-3670-9399; Rivadeneira, Fernando/0000-0001-9435-9441; Osborne, Nicholas/0000-0002-6700-2284; mangino, massimo/0000-0002-2167-7470; Soranzo, Nicole/0000-0003-1095-3852; smyth, deborah/0000-0002-6330-2669; Hocking, Lynne J/0000-0002-2414-2826; Ramachandran, Vasan/0000-0001-7357-5970 FU Novartis; Roche; Amgen; NIH/NHLBI [N01-HC-25195]; American Heart Association; US Department of Agriculture, Agricultural Research Service [58-1950-7-707]; National Institute of Aging [AG14759]; National Institute of Arthritis, Musculoskeletal, and Skin Diseases; National Institute on Aging [R01 AR/AG 41398]; Affymetrix Inc [N02-HL-6-4278]; Robert Dawson Evans Endowment of the Department of Medicine at Boston University School of Medicine and Boston Medical Center; Wellcome Trust; Arthritis Research Campaign; European Community [FP7/2007-2013, HEALTH-F2-2008-201865-GEFOS, 200800, OA /(FP7/2007-2013), HEALTH-F4-2007-201413, QLG2-CT-2002-01254]; UK Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre; Biotechnology and Biological Sciences Research Council [G20234]; National Eye Institute (NEI) via an NIH/Center for Inherited Disease Research (CIDR); Netherlands Organization of Scientific Research (NWO) [175.010.2005.011, 911-03-012]; Research Institute for Diseases in the Elderly [014-93-015: RIDE2]; Netherlands Genomics Initiative/NWO [050-060-810]; European Commission [HEALTH-F2-2008-201865-GEFOS, HEALTH-F2-2008-00-TREAT-OA]; Erasmus Medical Center and Erasmus University, Rotterdam, Netherlands Organization for the Health Research and Development; Research Institute for Diseases in the Elderly; Ministry of Education, Culture and Science; Ministry for Health, Welfare and Sports; European Commission; Municipality of Rotterdam FX TJW has served on the scientific advisory board of Diasorin. DKH has received honoraria from Abbott Nutrition. MW has received consultancy fees, honoraria, and speakers' fees from Abbott and Genzyme. DMR has acted as a consultant for Novartis, Roche, Pfizer, Amgen, Shire, Merck, and Servier, has received speakers' fees from Novartis, Roche, and Amgen, and owns stock in GlaxoSmithKline and Astra Zeneca. ML(2) has received lecture fees from Novartis and Sanofi-Aventis. All other authors declare that they have no conflicts of interest.; Framingham Heart Study The Framingham Heart Study of the National Heart, Lung and Blood Institute (NHLBI) of the US National Institutes of Health (NIH) and Boston University School of Medicine is supported by the NIH/NHLBI contract N01-HC-25195. The present study received support from the American Heart Association, the US Department of Agriculture, Agricultural Research Service (under Cooperative Agreement No 58-1950-7-707), and the National Institute of Aging (AG14759). DK was supported by a grant from the National Institute of Arthritis, Musculoskeletal, and Skin Diseases and the National Institute on Aging (R01 AR/AG 41398). The analyses reflect intellectual input and resource development from the Framingham Heart Study investigators participating in the SNP Health Association Resource project. This work was partly supported by a contract with Affymetrix Inc for genotyping services (Contract No N02-HL-6-4278). A portion of this research used the Linux Cluster for Genetic Analysis, which is funded by the Robert Dawson Evans Endowment of the Department of Medicine at Boston University School of Medicine and Boston Medical Center. Twins UK and Chingford The study was funded by the Wellcome Trust, Arthritis Research Campaign, European Community's Seventh Framework Programme (FP7/2007-2013)/grant agreement HEALTH-F2-2008-201865-GEFOS and Seventh Framework Programme grant 200800 Treat OA /(FP7/2007-2013), ENGAGE project grant agreement HEALTH-F4-2007-201413, and the FP.5 GenomEUtwin Project (QLG2-CT-2002-01254). The study also receives support from the UK Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre award to Guy's & St Thomas' NHS Foundation Trust in partnership with King's College London. TDS is an NIHR senior investigator. The project also received support from a Biotechnology and Biological Sciences Research Council project grant. (G20234). The authors acknowledge the funding and support of the National Eye Institute (NEI) via an NIH/Center for Inherited Disease Research (CIDR) genotyping project. We thank the staff from the Genotyping Facilities at the Wellcome Trust Sanger Institute for sample preparation, quality control, and genotyping; Le Centre National de Genotypage, France, for genotyping; Duke University, NC, USA, for genotyping; and the Finnish Institute of Molecular Medicine, Finnish Genome Center, University of Helsinki. Genotyping was also done by CIDR as part of an NEI/NIH project grant. The Rotterdam Study This study was funded by the Netherlands Organization of Scientific Research (NWO) Investments (175.010.2005.011, 911-03-012), the Research Institute for Diseases in the Elderly (014-93-015: RIDE2), the Netherlands Genomics Initiative/NWO project 050-060-810, and the European Commission (HEALTH-F2-2008-201865-GEFOS, and HEALTH-F2-2008-00-TREAT-OA). The Rotterdam Study is funded by Erasmus Medical Center and Erasmus University, Rotterdam, Netherlands Organization for the Health Research and Development, the Research Institute for Diseases in the Elderly, the Ministry of Education, Culture and Science, the Ministry for Health, Welfare and Sports, the European Commission (DG XII), and the Municipality of Rotterdam. We thank the staff from the Rotterdam Study, and the participating general practitioners and pharmacists. NR 34 TC 610 Z9 625 U1 3 U2 49 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0140-6736 J9 LANCET JI Lancet PD JUL 17 PY 2010 VL 376 IS 9736 BP 180 EP 188 DI 10.1016/S0140-6736(10)60588-0 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA 630YP UT WOS:000280313100025 PM 20541252 ER PT J AU Gorniak, S Gotardo, A Pfister, J AF Gorniak, S. Gotardo, A. Pfister, J. TI The effects of Ipomoea carnea on neonate behavior: A study in goats SO TOXICOLOGY LETTERS LA English DT Meeting Abstract C1 [Gorniak, S.; Gotardo, A.] Univ Sao Paulo, Sch Vet Med, BR-05508 Sao Paulo, Brazil. [Pfister, J.] USDA ARS, Poisonous Plant Res Lab, Washington, DC 20250 USA. RI Gorniak, Silvana/C-7879-2013; Gotardo, Andre/G-3788-2013 OI Gotardo, Andre/0000-0001-7297-0522 NR 0 TC 2 Z9 4 U1 0 U2 0 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD JUL 17 PY 2010 VL 196 SU S BP S186 EP S186 DI 10.1016/j.toxlet.2010.03.634 PG 1 WC Toxicology SC Toxicology GA V25II UT WOS:000208471301108 ER PT J AU Li, MHH Robinson, EH Bosworth, BG Oberle, DF Lucas, PM AF Li, Menghe H. Robinson, Edwin H. Bosworth, Brian G. Oberle, Daniel F. Lucas, Penelope M. TI Effects of varying dietary compositions using common feed ingredients on growth and feed efficiency of pond-raised channel catfish Ictalurus punctatus (Rafinesque) SO AQUACULTURE RESEARCH LA English DT Article DE channel catfish; dietary composition; growth; feed efficiency ID ANIMAL PROTEIN-SOURCES; SOYBEAN-MEAL; FISH-MEAL; BODY-COMPOSITION; COTTONSEED MEAL; PERFORMANCE; REPLACEMENT AB Two experiments were conducted to evaluate various ingredient combinations in a 28% or 32% protein diet for optimum performance of channel catfish Ictalurus punctatus. All diets contained soybean meal and corn, but with or without cottonseed meal, wheat middlings or fish meal (FM). Channel catfish fingerlings were stocked into 0.04 ha earthen ponds at 18 530 fish ha-1. Fish were fed one of eight diets once daily to apparent satiation for two growing seasons. Results demonstrated that the dietary ingredient composition used had significant effects on fish performance, but magnitude of differences was relatively small. Overall, diets containing FM resulted in greater weight gain (Experiments 1 and 2) and lower feed conversion ratio (Experiment 1) than fish fed all-plant diets. However, certain combinations of plant ingredients provided the similar fish growth as some diets containing FM. There were no significant differences in weight gain between fish fed soybean meal-corn or soybean meal-corn-wheat middlings-based diets with cottonseed meal or FM. The use of wheat middlings in the diet had no significant effects on fish production characteristics. C1 [Li, Menghe H.; Robinson, Edwin H.; Oberle, Daniel F.; Lucas, Penelope M.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS USA. [Bosworth, Brian G.] ARS, USDA, Catfish Genet Res Unit, Stoneville, MS USA. RP Li, MHH (reprint author), Natl Warmwater Aquaculture Ctr, POB 197, Stoneville, MS 38776 USA. EM mli@drec.msstate.edu FU MAFES [MIS-081111] FX The authors thank Dwayne Holifield, Charles Manning and staff at the Delta Western Research Center for assistance in feeding and pond management. This manuscript is approved for publication as Journal Article No. J-11624 of the Mississippi Agricultural and Forestry Experiment Station (MAFES), Mississippi State University. This project is supported under MAFES Project Number MIS-081111. NR 22 TC 2 Z9 2 U1 1 U2 5 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1355-557X J9 AQUAC RES JI Aquac. Res. PD JUL 16 PY 2010 VL 41 IS 8 BP 1133 EP 1139 DI 10.1111/j.1365-2109.2009.02400.x PG 7 WC Fisheries SC Fisheries GA 625YG UT WOS:000279932200003 ER PT J AU Fuller, SA McEntire, M Ludwig, GM AF Fuller, Sidney Adam McEntire, Matthew Ludwig, Gerald M. TI Development and testing of a pedigree-marking system using visible implant elastomer tags for selective improvement in Morone breeding programmes SO AQUACULTURE RESEARCH LA English DT Article DE visible implant elastomer tags; Morone; sunshine bass; white bass; selective breeding programmes ID GROWTH; RETENTION; FISH; PERCH AB The development and testing of a visible implant elastomer pedigree-marking system was evaluated in sunshine bass, Morone chrysops x Morone saxatilis, and white bass, M. chrysops (Rafinesque). These tags were tested in sunshine bass fingerlings at one of four subdermal body locations (posterior to the eye, dorsal fin musculature, caudal fin musculature or anal fin musculature). Tag visibility decreased with increased sunshine bass growth (63% after 56 days). Visibility differed among body locations, with the caudal and anal tagging locations having lower visibility. White bass fingerlings representing eight genetic groups were then tagged at one of two body locations (left or right subdermal along the dorsal musculature) using one of four fluorescent colours and reared for 42 days in a common garden growth trial. Tag visibility in white bass was 99.5% at 14 days, 98.2% at 28 days and 94.9% at 42 days after tagging. There was a significant change in weight among the eight genetic groups of white bass fingerlings after 42 days (P=0.03). Testing of this pedigree tagging system successfully identified phenotypically different groups of white bass fingerlings. C1 [Fuller, Sidney Adam; McEntire, Matthew; Ludwig, Gerald M.] ARS, USDA, Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Fuller, SA (reprint author), ARS, USDA, Stuttgart Natl Aquaculture Res Ctr, POB 1050,2955 Highway 130 E, Stuttgart, AR 72160 USA. EM adam.fuller@ars.usda.gov NR 20 TC 0 Z9 0 U1 0 U2 2 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1355-557X J9 AQUAC RES JI Aquac. Res. PD JUL 16 PY 2010 VL 41 IS 8 BP 1250 EP 1254 DI 10.1111/j.1365-2109.2009.02412.x PG 5 WC Fisheries SC Fisheries GA 625YG UT WOS:000279932200015 ER PT J AU Glenn, AE Karagianni, EP Ulndreaj, F Boukouvala, S AF Glenn, Anthony E. Karagianni, Eleni P. Ulndreaj, Fntigona Boukouvala, Sotiria TI Comparative genomic and phylogenetic investigation of the xenobiotic metabolizing arylamine N-acetyltransferase enzyme family SO FEBS LETTERS LA English DT Article DE Arylamine N-acetyltransferases; Xenobiotic metabolizing enzymes; Archaea; Fungi; Protista; Phylogeny ID EUKARYOTIC GENOMES; GENE AB Arylamine N-acetyltransferases (NATs) are xenobiotic metabolizing enzymes characterized in several bacteria and eukaryotic organisms. We report a comprehensive phylogenetic analysis employing an exhaustive dataset of NAT-homologous sequences recovered through inspection of 2445 genomes. We describe the first NAT homologues in viruses, archaea, protists, many fungi and invertebrates, providing complete annotations in line with the consensus nomenclature. Contrary to the NAT genes of vertebrates, introns are commonly found within the homologous coding regions of lower eukaryotes. The NATs of fungi and higher animals are distinctly monophyletic, but evidence supports a mixed phylogeny of NATs among bacteria, protists and possibly some invertebrates. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. C1 [Karagianni, Eleni P.; Ulndreaj, Fntigona; Boukouvala, Sotiria] Democritus Univ Thrace, Dept Mol Biol & Genet, Alexandroupolis 68100, Greece. [Glenn, Anthony E.] ARS, Toxicol & Mycotoxin Res Unit, Russell Res Ctr, USDA, Athens, GA 30605 USA. RP Boukouvala, S (reprint author), Democritus Univ Thrace, Dept Mol Biol & Genet, Alexandroupolis 68100, Greece. EM sboukouv@mbg.duth.gr FU Bodossaki Foundation (Greece) FX We wish to acknowledge the NAT Gene Nomenclature Committee. We also thank Irene Vagena for supporting the training of E.P.K. and A.U. in data mining and analysis, and the Bodossaki Foundation (Greece) for funding. NR 9 TC 12 Z9 12 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD JUL 16 PY 2010 VL 584 IS 14 BP 3158 EP 3164 DI 10.1016/j.febslet.2010.05.063 PG 7 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 619OF UT WOS:000279438800033 PM 20621844 ER PT J AU Richardson, MC Mitchell, CPJ Branfireun, BA Kolka, RK AF Richardson, Murray C. Mitchell, Carl P. J. Branfireun, Brian A. Kolka, Randall K. TI Analysis of airborne LiDAR surveys to quantify the characteristic morphologies of northern forested wetlands SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID DISSOLVED ORGANIC-CARBON; NET METHYLMERCURY PRODUCTION; BIOGEOCHEMICAL HOT-SPOTS; PORE-WATER CHEMISTRY; CANADIAN SHIELD; METHYL MERCURY; PEATLANDS; HYDROLOGY; EXPORT; SULFATE AB A new technique for quantifying the geomorphic form of northern forested wetlands from airborne LiDAR surveys is introduced, demonstrating the unprecedented ability to characterize the geomorphic form of northern forested wetlands using high-resolution digital topography. Two quantitative indices are presented, including the lagg width index (LWI) which objectively quantifies the lagg width, and the lateral slope index (LSI) which is a proxy measurement for the dome shape or convexity of the wetland ground surface. For 14 forested wetlands in central Ontario, Canada, northwestern Ontario, Canada, and northern Minnesota, United States, these indices were systematically correlated to metrics of topographic setting computed from LiDAR digital elevation models. In particular, these indices were strongly correlated with a Peatland Topographic Index (PTI, r(2) = 0.58 and r(2) = 0.64, respectively, p <= 0.001) describing the relative influence of upslope contributing area on the hydrology and biogeochemistry of individual wetlands. The relationship between PTI and the LWI and LSI indices was interpreted as geomorphic evolution in response to the spatially varying influence of upslope runoff on subsurface hydrochemistry. Spatial patterns of near-surface pore water chemistry were consistent with this interpretation. Specifically, at four wetland sites sampled extensively for pore water chemistry, the mean and variance of near-surface pore water methylmercury (MeHg) concentrations were higher within the zone of enhanced upland-wetland interactions, as inferred from the LiDAR-derived LWI estimates. Use of LiDAR surveys to measure subtle topographic gradients within wetlands may therefore help quantify the influence of upland-wetland interactions on biogeochemical cycling and export in northern forested landscapes. C1 [Richardson, Murray C.; Branfireun, Brian A.] Univ Toronto, Dept Geog, Mississauga, ON L5L 1C6, Canada. [Kolka, Randall K.] US Forest Serv, No Res Stn, USDA, Grand Rapids, MN 55744 USA. [Mitchell, Carl P. J.] Univ Toronto Scarborough, Dept Phys & Environm Sci, Toronto, ON M1C 1A4, Canada. RP Richardson, MC (reprint author), Carleton Univ, Dept Geog & Environm Studies, Ottawa, ON K1S 5B6, Canada. EM murray_richardson@carleton.ca RI Mitchell, Carl/A-7212-2008 OI Mitchell, Carl/0000-0001-8538-5138 FU Natural Sciences and Engineering Research Council FX We gratefully acknowledge financial support for this research from the Natural Sciences and Engineering Research Council in the form a NSERC CGS to MCR and a NSERC discovery grant to BAB. We would also like to thank Chris Hopkinson and Laura Chasmer of the Applied Geomatics Research Group for conducting the LIDAR surveys at the Muskoka-Haliburton sites. NR 51 TC 11 Z9 11 U1 1 U2 23 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0148-0227 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUL 16 PY 2010 VL 115 AR G03005 DI 10.1029/2009JG000972 PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 627NX UT WOS:000280047700001 ER PT J AU Cannon, SB Ilut, D Farmer, AD Maki, SL May, GD Singer, SR Doyle, JJ AF Cannon, Steven B. Ilut, Dan Farmer, Andrew D. Maki, Sonja L. May, Gregory D. Singer, Susan R. Doyle, Jeff J. TI Polyploidy Did Not Predate the Evolution of Nodulation in All Legumes SO PLOS ONE LA English DT Article ID MEDICAGO-TRUNCATULA; GENE DUPLICATION; WHOLE-GENOME; PHYLOGENY; DIVERSIFICATION; PALEOPOLYPLOIDY; LEGUMINOSAE; ANGIOSPERMS; SEQUENCES; FAMILY AB Background: Several lines of evidence indicate that polyploidy occurred by around 54 million years ago, early in the history of legume evolution, but it has not been known whether this event was confined to the papilionoid subfamily (Papilionoideae; e. g. beans, medics, lupins) or occurred earlier. Determining the timing of the polyploidy event is important for understanding whether polyploidy might have contributed to rapid diversification and radiation of the legumes near the origin of the family; and whether polyploidy might have provided genetic material that enabled the evolution of a novel organ, the nitrogen-fixing nodule. Although symbioses with nitrogen-fixing partners have evolved in several lineages in the rosid I clade, nodules are widespread only in legume taxa, being nearly universal in the papilionoids and in the mimosoid subfamily (e. g., mimosas, acacias) - which diverged from the papilionoid legumes around 58 million years ago, soon after the origin of the legumes. Methodology/Principal Findings: Using transcriptome sequence data from Chamaecrista fasciculata, a nodulating member of the mimosoid clade, we tested whether this species underwent polyploidy within the timeframe of legume diversification. Analysis of gene family branching orders and synonymous-site divergence data from C. fasciculata, Glycine max (soybean), Medicago truncatula, and Vitis vinifera (grape; an outgroup to the rosid taxa) establish that the polyploidy event known from soybean and Medicago occurred after the separation of the mimosoid and papilionoid clades, and at or shortly before the Papilionoideae radiation. Conclusions: The ancestral legume genome was not fundamentally polyploid. Moreover, because there has not been an independent instance of polyploidy in the Chamaecrista lineage there is no necessary connection between polyploidy and nodulation in legumes. Chamaecrista may serve as a useful model in the legumes that lacks a paleopolyploid history, at least relative to the widely studied papilionoid models. C1 [Cannon, Steven B.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genom Res Unit, Ames, IA 50011 USA. [Ilut, Dan; Doyle, Jeff J.] Cornell Univ, Dept Plant Biol, Ithaca, NY USA. [Farmer, Andrew D.; May, Gregory D.] Natl Ctr Genome Resources, Santa Fe, NM USA. [Maki, Sonja L.; Singer, Susan R.] Carleton Coll, Dept Biol, Northfield, MN 55057 USA. RP Cannon, SB (reprint author), Iowa State Univ, USDA ARS, Corn Insects & Crop Genom Res Unit, Ames, IA 50011 USA. EM steven.cannon@ars.usda.gov OI Ilut, Daniel/0000-0001-7497-9109 FU National Science Foundation [DEB-0746571, DUE-0837375] FX Supported by the National Science Foundation (grants DEB-0746571 and DUE-0837375). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 37 Z9 38 U1 0 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 16 PY 2010 VL 5 IS 7 AR e11630 DI 10.1371/journal.pone.0011630 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 626QE UT WOS:000279980800018 PM 20661290 ER PT J AU Kim, KS Taylor, SE Gleason, ML Nutter, FW Coop, LB Pfender, WF Seem, RC Sentelhas, PC Gillespie, TJ Marta, AD Orlandini, S AF Kim, Kwang Soo Taylor, S. Elwynn Gleason, Mark L. Nutter, Forrest W., Jr. Coop, Leonard B. Pfender, William F. Seem, Robert C. Sentelhas, Paulo C. Gillespie, Terry J. Marta, Anna Dalla Orlandini, Simone TI Spatial portability of numerical models of leaf wetness duration based on empirical approaches SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Surface wetness; Empirical model; Fuzzy logic; RH; Leaf wetness duration ID SURFACE WETNESS; WARNING SYSTEM; PLANT-DISEASE; SOOTY BLOTCH; DEW DURATION; PERFORMANCE; VALIDATION; SIMULATION; RADIATION; FLYSPECK AB Leaf wetness duration (LWD) models based on empirical approaches offer practical advantages over physically based models in agricultural applications, but their spatial portability is questionable because they may be biased to the climatic conditions under which they were developed. In our study, spatial portability of three LWD models with empirical characteristics - a RH threshold model, a decision tree model with wind speed correction, and a fuzzy logic model - was evaluated using weather data collected in Brazil, Canada, Costa Rica, Italy and the USA. The fuzzy logic model was more accurate than the other models in estimating LWD measured by painted leaf wetness sensors. The fraction of correct estimates for the fuzzy logic model was greater (0.87) than for the other models (0.85-0.86) across 28 sites where painted sensors were installed, and the degree of agreement k statistic between the model and painted sensors was greater for the fuzzy logic model (0.71) than that for the other models (0.64-0.66). Values of the k statistic for the fuzzy logic model were also less variable across sites than those of the other models. When model estimates were compared with measurements from unpainted leaf wetness sensors, the fuzzy logic model had less mean absolute error (2.5 h day(-1)) than other models (2.6-2.7 h day(-1)) after the model was calibrated for the unpainted sensors. The results suggest that the fuzzy logic model has greater spatial portability than the other models evaluated and merits further validation in comparison with physical models under a wider range of climate conditions. (C) 2010 Elsevier B.V. All rights reserved. C1 [Kim, Kwang Soo] New Zealand Inst Plant & Food Res Ltd, Mt Albert Res Ctr, Auckland, New Zealand. [Taylor, S. Elwynn] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Gleason, Mark L.; Nutter, Forrest W., Jr.] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA. [Seem, Robert C.] Cornell Univ, Dept Plant Pathol, NYSAES, Geneva, NY 14456 USA. [Coop, Leonard B.] Oregon State Univ, Dept Bot & Plant Pathol, Integrated Plant Protect Ctr, Corvallis, OR 97331 USA. [Pfender, William F.] ARS, USDA, NFSPRC, Corvallis, OR 97331 USA. [Marta, Anna Dalla; Orlandini, Simone] Univ Florence, Dept Plant Soil & Environm Sci, I-50144 Florence, Italy. [Gillespie, Terry J.] Univ Guelph, Ontario Agr Coll, Dept Land Resource Sci, Agrometeorol Grp, Guelph, ON N1G 2W1, Canada. [Sentelhas, Paulo C.] Univ Sao Paulo, Agrometeorol Grp, Dept Biosyst Engn, ESALQ, BR-13418900 Piracicaba, SP, Brazil. RP Kim, KS (reprint author), New Zealand Inst Plant & Food Res Ltd, Mt Albert Res Ctr, 120 Mt Albert Rd,Private Bag 92 169, Auckland, New Zealand. EM kwang.kim@plantandfood.co.nz RI Orlandini, Simone/L-7880-2015; Sentelhas, Paulo/B-7849-2012; OI Orlandini, Simone/0000-0001-6290-9752; Dalla Marta, Anna/0000-0002-4606-7521 FU New Zealand's Foundation for Research, Science and Technology [C06X0810]; USDA-RAMP [2005-51101-02384] FX This work was supported in part by New Zealand's Foundation for Research, Science and Technology through contract C06X0810, and USDA-RAMP competitive grant #2005-51101-02384. NR 36 TC 8 Z9 8 U1 0 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUL 15 PY 2010 VL 150 IS 7-8 BP 871 EP 880 DI 10.1016/j.agrformet.2010.02.006 PG 10 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 627XM UT WOS:000280077500002 ER PT J AU Heitman, JL Horton, R Sauer, TJ Ren, TS Xiao, X AF Heitman, J. L. Horton, R. Sauer, T. J. Ren, T. S. Xiao, X. TI Latent heat in soil heat flux measurements SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Soil heat flux; Latent heat flux; Surface energy balance ID ENERGY-BALANCE; THERMAL-CONDUCTIVITY; PULSE METHOD; SURFACE; PROBE; TEMPERATURE; VARIABILITY; BENEATH; CANOPY AB The surface energy balance includes a term for soil heat flux. Soil heat flux is difficult to measure because it includes conduction and convection heat transfer processes. Accurate representation of soil heat flux is an important consideration in many modeling and measurement applications. Yet, there remains uncertainty about what comprises soil heat flux and how surface and subsurface heat fluxes are linked in energy balance closure. The objective of this study is to demonstrate the presence of a subsurface latent heat sink, which must be considered in order to accurately link subsurface heat fluxes between depths near and at the soil surface. Measurements were performed under effectively bare surface conditions in a silty clay loam soil near Ames, IA. Soil heat flux was measured with heat-pulse sensors using the gradient heat flux approach at 1-, 3-, and 6-cm soil depths. Independent estimates of the daily latent heat sink were obtained by measuring the change of mass of microlysimeters. Heat flux measurements at the 1-cm depth deviated from heat flux measurements at other depths, even after calorimetric adjustment was made. This deviation was most pronounced shortly after rainfall, where the 1-cm soil heat flux measurement exceeded 400 W m(-2). Cumulative soil heat flux measurements at the 1-cm depth exceeded measurements at the 3-cm depth by >75% over a 7-day rain-free period, whereas calorimetric adjustment allowed 3- and 6-cm depth measurements to converge. latent heat sink estimates from the microlysimeters accounted for nearly all of the differences between the 1- and 3-cm depth heat flux measurements, indicating that the latent heat sink was distributed between the 1- and 3-cm depths shortly after the rainfall event. Results demonstrate the importance of including latent heat when attempts are made to link or extrapolate subsurface soil heat flux measurements to the surface soil heat flux. (C) 2010 Elsevier B.V. All rights reserved. C1 [Heitman, J. L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Horton, R.; Xiao, X.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Sauer, T. J.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Ren, T. S.] China Agr Univ, Dept Soil & Water, Beijing 100193, Peoples R China. RP Heitman, JL (reprint author), N Carolina State Univ, Dept Soil Sci, Campus Box 7619, Raleigh, NC 27695 USA. EM jlheitman@ncsu.edu RI Heitman, Joshua/F-8535-2010; Xiao, Xinhua/F-4285-2014 OI Xiao, Xinhua/0000-0002-0629-4391 FU National Science Foundation [0809656]; Chinese Academy of Sciences [2009Z2-37] FX The authors gratefully acknowledge the National Science Foundation for supporting this work through NSF Award No. 0809656. Work was also supported by the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists, Award No. 2009Z2-37. NR 30 TC 27 Z9 30 U1 1 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUL 15 PY 2010 VL 150 IS 7-8 BP 1147 EP 1153 DI 10.1016/j.agrformet.2010.04.017 PG 7 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 627XM UT WOS:000280077500026 ER PT J AU Luthria, D Singh, AP Wilson, T Vorsa, N Banuelos, GS Vinyard, BT AF Luthria, Devanand Singh, Ajay P. Wilson, Ted Vorsa, Nicholi Banuelos, Gary S. Vinyard, Bryan T. TI Influence of conventional and organic agricultural practices on the phenolic content in eggplant pulp: Plant-to-plant variation SO FOOD CHEMISTRY LA English DT Article DE Eggplant (Solanum melongena L.); Polyphenols; Conventional and organic; 5-Caffeoylqunic acid; HPLC and LC-MS analysis; Total phenolics; Folin-Ciocalteu; Plant-to-plant variations ID SAMPLE PREPARATION; EXTRACTION; FOODS; ISOFLAVONES; VEGETABLES; PEPPERS; DISEASE; FRUITS; ACIDS; L. AB Consumer awareness, pesticide and fertiliser contaminations, and environmental concerns have resulted in significant demand for organically grown farm products. The present study evaluates the influence that organic and conventional farming practices exert on the total phenolic content in eggplant samples from two cultivars, Blackbell (American eggplant) and Millionaire (Japanese eggplant), grown by conventional and organic farming practices with similar climatic conditions. In addition, plant-to-plant variation in phenolic content was determined from eight eggplant samples collected from different plants grown at various field positions. Samples were separately assayed for phenolic content by the two independent procedures, HPLC/LC-MS and Folin-Ciocalteu assay. The results of the HPLC analysis showed significant plant-to-plant variation (% RSD ranged from 22% to 48%) in 5-caffeoylqunic acid content (the most abundant phenolic acid) in eight eggplant samples collected from eight different plants belonging to the same cultivar and grown under similar conditions. The cv. Millionaire showed a higher total phenolic content compared to the Blackbell cultivar. We did not observe significant consistent trend in the phenolic content of eggplant samples grown with organic and conventional farming practices with both eggplant cultivars. We, however, observed a strong correlation (R(2) = 0.87) between total phenolic content as assayed by Folin-Ciocalteu assay and 5-caffeoylqunic acid, a major phenolic acid in eggplant extract. This study concludes that multiple repetitive analyses of plant products collected from different plants grown over various time periods (seasons) at different locations should always be carried to unambiguously prove the impact of growing conditions on phenolic content or antioxidant activity. Published by Elsevier Ltd. C1 [Luthria, Devanand] USDA, BHNRC, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. [Singh, Ajay P.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Wilson, Ted] Winona State Univ, Dept Biol, Winona, MN 55987 USA. [Vorsa, Nicholi; Banuelos, Gary S.] ARS, Water Management Res Lab, USDA, Parlier, CA 93648 USA. [Vinyard, Bryan T.] USDA, Biometr Consulting Serv, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Luthria, D (reprint author), USDA, BHNRC, Food Composit & Methods Dev Lab, 10300 Baltimore Ave,Bldg 161 BARC E, Beltsville, MD 20705 USA. EM D.Luthria@ars.usda.gov NR 25 TC 39 Z9 40 U1 0 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUL 15 PY 2010 VL 121 IS 2 BP 406 EP 411 DI 10.1016/j.foodchem.2009.12.055 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 575BK UT WOS:000276038400014 ER PT J AU Anderson, RS Smith, SJ Lynch, AM Geils, BW AF Anderson, R. Scott Smith, Susan J. Lynch, Ann M. Geils, Brian W. TI The pollen record of a 20th century spruce beetle (Dendroctonus rufipennis) outbreak in a Colorado subalpine forest, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Spruce beetle outbreak; Dendroctonus rufipennis; Pollen analysis; Fire history; Colorado ID EASTERN NORTH-AMERICA; MIDHOLOCENE HEMLOCK DECLINE; TREE-RING RECONSTRUCTION; SOUTHERN ROCKY-MOUNTAINS; CLIMATE-CHANGE; KENAI PENINSULA; RADIOCARBON AGE; LAKE-SEDIMENTS; UNITED-STATES; NATIONAL-PARK AB The frequency and intensity of ecosystem disturbance, including outbreaks of forest insects and forest fires, is expected to increase in the future as a result of higher temperatures and prolonged drought. While many studies have concentrated on the future climatic impacts on fire, little is known about the impact of future climate on insect infestation. Paleoecological techniques are important in this regard in identifying the potential relationships between climate and insect outbreaks in the past, as a predictive tool for the future. We examine a high-resolution 20th century record of spruce beetle (Dendroctonus rufipennis) infestation from a small, subalpine lake, comparing the paleoecological record to the historical and treering record of the event. An extensive spruce beetle outbreak occurred in northwestern Colorado during the 1940s and 1950s, causing widespread mortality of mature Picea engelmannii. Pollen analysis of this period documents the decline of Picea and its replacement locally by Abies lasiocarpa, paralleling age and composition studies of modern forest stands in the region. This study is a proof of concept that, when applied to longer sedimentary records, could produce a detailed record of infestation for the Late Holocene or older time periods. This information will be useful to forest managers in efforts to plan for the effects of D. rufipennis infestations, and subsequent succession within high elevation conifer forests. (C) 2010 Elsevier B.V. All rights reserved. C1 [Anderson, R. Scott] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. [Anderson, R. Scott; Smith, Susan J.] No Arizona Univ, Lab Paleoecol, Bilby Res Ctr, Flagstaff, AZ 86011 USA. [Lynch, Ann M.] Univ Arizona, Rocky Mt Res Stn, USDA, US Forest Serv, Tucson, AZ 85721 USA. [Lynch, Ann M.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Geils, Brian W.] US Forest Serv, USDA, Flagstaff, AZ 86001 USA. RP Anderson, RS (reprint author), No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. EM Scott.Anderson@nau.edu; Susan.Smith@nau.edu; alynch@fs.fed.us; bgeils@fs.fed.us FU USDA [28-JV7-949, 04-JV-11221615-317] FX We are grateful to Chris Black, John Wiley, Eric Feiler and Mike Timpson for assistance in sediment coring; Teh-Lung (Richard) Ku for analyzing the 210Pb and 137Cs profiles; Dan Boone for help in photographing the sediments; Kirsten Ironside for Fig. 1, and Tony DeLuz for Fig. 4; and Andrea Brunelle and one anonymous reviewer for their insightful comments. We also thank Jeff Overturf, Jerry Schmidt, Liz Mauch, and Robin Inhelder (US Forest Service) for their assistance. This research was funded, in part by cooperative agreements (USDA 28-JV7-949 and USDA 04-JV-11221615-317). Laboratory of Paleoecology Contribution # 127. NR 84 TC 8 Z9 10 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2010 VL 260 IS 4 BP 448 EP 455 DI 10.1016/j.foreco.2010.05.001 PG 8 WC Forestry SC Forestry GA 629CQ UT WOS:000280172400003 ER PT J AU Royse, J Arthur, MA Schorgendorfer, A Loftis, DL AF Royse, Jacob Arthur, Mary A. Schoergendorfer, Angela Loftis, David L. TI Establishment and growth of oak (Quercus alba, Quercus prinus) seedlings in burned and fire-excluded upland forests on the Cumberland Plateau SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Chestnut oak; White oak; Prescribed fire; Oak regeneration ID EASTERN NORTH-AMERICA; REPEATED PRESCRIBED FIRES; PINE PINUS-PUNGENS; ADVANCE REGENERATION; SOUTHERN APPALACHIANS; RED OAK; SURVIVAL; LITTER; SINGLE; STANDS AB Recurrent problems with regeneration of oaks (Quercus spp.) have been documented across a wide range of ecosystems. In oak-dominated forests of the central and Appalachian hardwood regions of the United States, a lack of competitive oak regeneration has been tied, in part, to fire suppression in these landscapes, and managers throughout the region are using prescribed fire to address this concern. To examine fire effects on oak regeneration, researchers have generally relied on inventories or population studies of existing seedlings. These studies are valuable but do not permit examination of the role of fire in enhancing the establishment and growth of new oak seedlings stemming from oak mast events. In this study, white (Quercus alba) and chestnut oak (Quercus prinus) acorn mast crops serendipitously occurred in year three (fall 2005) of a landscape-scale prescribed fire experiment. We examined establishment, survival, height and diameter of new seedlings on sites on the Cumberland Plateau in eastern Kentucky. Treatments were fire exclusion, a single prescribed fire (1x-burn; 2003), and repeated prescribed fire (3x-bum; 2003, 2004, and after acorn drop in 2006), all conducted in late spring. Initial densities of newly established chestnut and white oak seedlings were statistically similar across treatments (P = 0.42), despite fires on the 3x-burn site having occurred after acorns were on the ground. Oak seedling density was significantly predicted by oak basal area on all sites (R(2) = 0.12-0.46), except for chestnut oak on fire-excluded sites (R(2) = 0.04). Litter depth was less on 3x-burn sites compared to 1x-burn and fire-excluded sites, whereas canopy openness was greater on both burn treatments compared to fire-excluded sites. Seedling mortality was generally higher on fire-excluded sites compared to bum sites, especially for white oak. Oak seedling mortality in the first two growing seasons was significantly predicted by initial litter depth and open sky, with greater litter depth and lower percent open sky leading to higher mortality. In the third growing season none of the measured variables predicted chestnut oak seedling survival; for white oak, percent open sky remained a significant predictor of mortality. Initially, seedlings on the fire-excluded sites had similar height but smaller diameter; after three growing seasons there were few differences in seedling height or diameter among treatments. Our findings suggest a potential role for prescribed fire in establishing forest floor and light conditions that may enhance the success of new oak germinants, although different responses among species may suggest the need to target management for individual oak species. (C) 2010 Elsevier B.V. All rights reserved. C1 [Royse, Jacob; Arthur, Mary A.] Univ Kentucky, Dept Forestry, Lexington, KY 40546 USA. [Royse, Jacob] US Forest Serv, London, KY 40744 USA. [Schoergendorfer, Angela] Univ Kentucky, Dept Stat, Lexington, KY 40506 USA. [Loftis, David L.] US Forest Serv, So Res Stn, Asheville, NC 28806 USA. RP Arthur, MA (reprint author), Univ Kentucky, Dept Forestry, 205 Thomas Poe Cooper Bldg, Lexington, KY 40546 USA. EM jroyse@fs.fed.us; marthur@uky.edu; angela.sch@uky.edu; dloftis@fs.fed.us OI Schorgendorfer, Angela/0000-0001-8017-3304 FU USDA-USDI [01-3-3-14, 04-2-1-06] FX This research was supported by the USDA-USDI Joint Fire Science Program (01-3-3-14, 04-2-1-06) through a cooperative research agreement with the USDA Forest Service Southern Research Station. We are grateful to the many people, too numerous to mention individually, who provided field support for this research. Special thanks, however, are warranted to Heather Alexander, Autumn Foushee, Jessi Lyons, and Gretchen Sovkoplas. Beth Blankenship provided essential technical editing and graphical support. We are also indebted to our USDA Forest Service collaborators from the Cumberland District of the Daniel Boone National Forest, including Jeff Lewis, EJ Bunzendahl, and David Manner. This study (#09-09-109) is connected with a project of the Kentucky Agricultural Experiment Station. This manuscript was greatly improved through the careful reviews and constructive comments provided by Jean-Paul Laclau and two anonymous reviewers. NR 53 TC 12 Z9 13 U1 5 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2010 VL 260 IS 4 BP 502 EP 510 DI 10.1016/j.foreco.2010.05.005 PG 9 WC Forestry SC Forestry GA 629CQ UT WOS:000280172400009 ER PT J AU Zhang, C Tian, HQ Wang, YH Zeng, T Liu, YQ AF Zhang, Chi Tian, Hanqin Wang, Yuhang Zeng, Tao Liu, Yongqiang TI Predicting response of fuel load to future changes in climate and atmospheric composition in the Southern United States SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Climate change; Dynamic Land Ecosystem Model (DLEM); Fuel load; Southern United States ID CHARACTERISTIC CLASSIFICATION-SYSTEM; PRODUCTIVITY; IMPACTS; MODELS; OZONE; FIRE; CO2; USA AB The model projected ecosystem carbon dynamics were incorporated into the default (contemporary) fuel load map developed by FCCS (Fuel Characteristic Classification System) to estimate the dynamics of fuel load in the Southern United States in response to projected changes in climate and atmosphere (CO(2) and nitrogen deposition) from 2002 to 2050. The study results indicated that in 2002 the total fuel load of the Southern United States was about 1.15 P g (1 P=10(15)). which will decrease to 1.11 P g in 2050. The declination of fuel load is mainly due to the climate change, especially the reduced precipitation in 2050, while the effects of elevated CO2 and nitrogen deposition will increase fuel load. Interactions among all factors will result in 1% reduction in the fuel load in 2050. In response to the spatial heterogeneity in environmental changes, the dynamics of fuel load from 2002 to 2050 vary strongly among the study states. The declined precipitation in the northern inland of the study region may lead to 20% fuel load reduction in Tennessee and Kentucky by the year of 2050, while the elevated precipitation and decreased daily mean temperature in the coastal states, especially in South Carolina, North Carolina, and Virginia, may result in fuel load accumulation. The temporal spatial variation of the fuel load may be overestimated since the adjustments of forest management regime in response to climate change were not considered in current study. (C) 2010 Elsevier B.V. All rights reserved. C1 [Zhang, Chi; Tian, Hanqin] Auburn Univ, Ecosyst Dynam & Global Ecol Lab, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Zhang, Chi] Arizona State Univ, Global Inst Sustainabil, Tempe, AZ 85281 USA. [Wang, Yuhang; Zeng, Tao] Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Forestry Sci Lab, Athens, GA 30602 USA. RP Tian, HQ (reprint author), Auburn Univ, Ecosyst Dynam & Global Ecol Lab, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. EM tianhan@auburn.edu RI Tian, Hanqin/A-6484-2012; Wang, Yuhang/B-5578-2014 OI Tian, Hanqin/0000-0002-1806-4091; FU U.S. Environmental Protection Agency (EPA) [2004-STAR-L1]; U.S. Department of Energy NICCR [DUKE-UN-07-SC-NICCR-1016] FX This research has been supported by the U.S. Environmental Protection Agency (EPA) Project 2004-STAR-L1 and U.S. Department of Energy NICCR Program (DUKE-UN-07-SC-NICCR-1016). NR 37 TC 14 Z9 14 U1 0 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2010 VL 260 IS 4 BP 556 EP 564 DI 10.1016/j.foreco.2010.05.012 PG 9 WC Forestry SC Forestry GA 629CQ UT WOS:000280172400016 ER PT J AU O'Donnell, TK Goyne, KW Miles, RJ Baffaut, C Anderson, SH Sudduth, KA AF O'Donnell, T. Kevin Goyne, Keith W. Miles, Randall J. Baffaut, Claire Anderson, Stephen H. Sudduth, Kenneth A. TI Identification and quantification of soil redoximorphic features by digital image processing SO GEODERMA LA English DT Article DE Digital photography; Soil redoximorphic features; Soil color; Image classification; Pedology; Hydric soils ID ELECTRICAL-CONDUCTIVITY; MORPHOLOGICAL FEATURES; SPATIAL-ANALYSIS; ORGANIC-MATTER; THIN-SECTIONS; COLOR; WATER; DURATION; MICROMORPHOLOGY; SATURATION AB Soil redoximorphic features (SRFs) have provided scientists and land managers with insight into relative soil moisture for approximately 60 years. The overall objective of this study was to develop a new method of SRF identification and quantification from soil cores using a digital camera and image classification software. Additional objectives included a determination of soil moisture effects on quantified SRFs and image processing effects on interpretation of SRF metrics. Eighteen horizons from selected landscapes in the Central Claypan Area, northcentral Missouri, USA were photographed from exposed soil cores under controlled light conditions. A 20 cm(2) area was used for SRF quantification following a determination of the initial gravimetric water content of horizon faces. Overall color determination accuracy was 99.6% based on Munsell soil color groupings used for SRF identification. Rewetting of air-dry horizon faces by successive application of 1 mL of deionized water demonstrated little change in identified SRFs after seven applications. Mean change in identified Low Chroma and High Chroma SRFs between the seventh and tenth rewetting sequences was 2% (SD +/- 4) and 0.03% (SD +/- 0.3), respectively. However, ten of eighteen horizons contained a greater area of Low Chroma after ten rewetting sequences compared to the same horizon at the initial moisture state. Metrics characterizing SRF boundaries, shapes, number of SRFs, and mean area of SRFs were sensitive to post-classification image smoothing. Methods demonstrated by this study provide an opportunity to better integrate pedology with other related earth sciences by allowing standardized quantification of SRFs as well as a determination of human error associated with current visual estimates. (C) 2010 Elsevier B.V. All rights reserved. C1 [O'Donnell, T. Kevin; Goyne, Keith W.; Miles, Randall J.; Anderson, Stephen H.] Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. [Baffaut, Claire; Sudduth, Kenneth A.] Univ Missouri, USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO 65211 USA. RP O'Donnell, TK (reprint author), Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. EM tkot24@mail.mizzou.edu FU University of Missouri Research Council [URC-08-046]; USDA-CSREES [2005-51130-02380] FX Funding for this research was provided by the University of Missouri Research Council (Grant # URC-08-046) and the USDA-CSREES, National Integrated Water Quality - Conservation Effects Assessment Project (CEAP) (Grant # 2005-51130-02380). NR 50 TC 6 Z9 7 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 J9 GEODERMA JI Geoderma PD JUL 15 PY 2010 VL 157 IS 3-4 BP 86 EP 96 DI 10.1016/j.geoderma.2010.03.019 PG 11 WC Soil Science SC Agriculture GA 623NT UT WOS:000279749800003 ER PT J AU Kishne, AS Morgan, CLS Ge, YF Miller, WL AF Kishne, Andrea Sz. Morgan, Cristine L. S. Ge, Yufeng Miller, Wesley L. TI Antecedent soil moisture affecting surface cracking of a Vertisol in field conditions SO GEODERMA LA English DT Article DE Vertisol; Shrinkage crack; Soil water; Hysteresis; Recharge ID SWELLING CLAY SOILS; TEXAS GULF-COAST; WATER-CONTENT; SHRINKAGE CURVE; PREFERENTIAL FLOW; MARSH SOIL; INFILTRATION; MODEL; CORES; VARIABILITY AB Cracking of shrink-swell soils influences landscape hydrology. Watershed models that address soil cracking phenomena generally use a relationship between shrinkage and current soil water content to estimate the extent of cracking. Although antecedent soil moisture prior to soil shrinkage is found to affect the shrinking of expansive soils in laboratory measurements, field observations are limited. In a previous study, a series of in situ surface crack measurements over 10 years indicated the effect of soil moisture just prior to the start of cracking (antecedent soil moisture) on cracking extent, but this relationship was not specifically analyzed. The objectives of this study were (i) to estimate the antecedent soil water content prior to cracking, (ii) to analyze the effect of antecedent moisture on crack area density in microhighs and microlows, and (iii) to assess the temporal distribution of antecedent soil moisture in relation to an estimated water availability index. Soil cracking was measured on a 10-m x 10-m plot of Laewest clay (fine, smectitic, hyperthermic Typic Hapludert) covered with native tallgrass vegetation on 42 dates during 1989-1998. Gravimetric soil water content was measured on 50 dates: 18 dates corresponded to crack measurements. Gilgai microtopograhy was mapped, and surface crack area density was calculated. For days when soil water content was not measured, it was estimated from precipitation and evapotranspiration. Antecedent soil water content prior to cracking was estimated for depth at 10 cm using daily estimates of soil water content and field notes on cracking. Results indicated that the temporal variation in surface crack area density of the study area during 10 years was related to dynamics of current and antecedent soil water content on microhighs and microlows (R-2=0.68 and 0.59, respectively). Prediction accuracies improved with classifying drying-wetting conditions during cracking. Dynamic temporal changes in the surface crack area density exhibited dependence on a long-term (multi-year) cycle of antecedent soil water content superimposed by short-term (within a year) cycles of current soil water content. (C) 2010 Elsevier B.V. All rights reserved. C1 [Kishne, Andrea Sz.; Morgan, Cristine L. S.; Ge, Yufeng] Texas A&M Univ, College Stn, TX 77843 USA. [Miller, Wesley L.] USDA NRCS, Victoria, TX USA. RP Kishne, AS (reprint author), Texas A&M Univ, College Stn, TX 77843 USA. EM akishne@tamu.edu RI Morgan, Cristine`/A-1555-2013 OI Morgan, Cristine`/0000-0001-9836-0669 FU Texas AgriLife Research; Texas USDA-NRCS FX This research was funded by a cooperative agreement between Texas AgriLife Research, and the Texas USDA-NRCS. The authors are thankful to Drs. K. McInnes and J. Heilman for comments on the calculation of water availability index and the manuscript. NR 74 TC 10 Z9 11 U1 2 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JUL 15 PY 2010 VL 157 IS 3-4 BP 109 EP 117 DI 10.1016/j.geoderma.2010.03.020 PG 9 WC Soil Science SC Agriculture GA 623NT UT WOS:000279749800005 ER PT J AU Reinstein, SL Lucio-Forster, A Bowman, DD Eberhard, ML Hoberg, EP Pot, SA Miller, PE AF Reinstein, Shelby L. Lucio-Forster, Araceli Bowman, Dwight D. Eberhard, Mark L. Hoberg, Eric P. Pot, Simon A. Miller, Paul E. TI Surgical extraction of an intraocular infection of Parelaphostrongylus tenuis in a horse SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Article ID CANINE NEURAL ANGIOSTRONGYLOSIS; NORTH-AMERICA; ELAPHOSTRONGYLINAE NEMATODA; MENINGEAL WORM; PROTOSTRONGYLIDAE; DIAGNOSIS; LARVAE AB Case Description-A 4-year-old Hanoverian gelding was evaluated because of a mobile worm-like structure in the right eye. Clinical Findings-Ophthalmologic examination of the right eye revealed a white, thin, coiled, mobile parasite, which was presumed to be a nematode, located in the ventral portion of the anterior chamber of the eye; there also were vitreal strands located temporally and inferiorly near the margin of the pupil. Results of ophthalmologic examination of the left eye were unremarkable. Treatment and Outcome-The horse was treated with a neomycin-polymyxin B-dexamethasone ophthalmic solution applied topically (1 drop, q 8 h) to the right eye and penicillin V potassium (22,000 U/kg [10,000 U/lb], IV, q 6 h). The horse was anesthetized. A stab incision was made in the cornea, and a viscoelastic agent was infused around the parasite. The parasite was extracted via the incision by use of an iris hook and tying forceps. The horse had an uncomplicated recovery from the procedure and retained vision in the right eye. Gross and microscopic examination was used to identify the parasite as an adult meta-strongyloid nematode consistent with a fully developed male Parelaphostrongylus tenuis. Clinical Relevance-To the authors' knowledge, this is the first report of intraocular parelaphostrongylosis in a horse. This report provided evidence that vision could be retained after treatment for intraocular P tenuis infection in a horse. (J Am Vet Med Assoc 2010;237:196-199) C1 [Reinstein, Shelby L.; Pot, Simon A.; Miller, Paul E.] Univ Wisconsin, Sch Vet Med, Dept Surg Sci, Madison, WI 53706 USA. [Lucio-Forster, Araceli; Bowman, Dwight D.] Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USA. [Eberhard, Mark L.] CDC, Div Parasit Dis, Atlanta, GA 30341 USA. [Hoberg, Eric P.] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20715 USA. RP Reinstein, SL (reprint author), Univ Penn, New Bolton Ctr, Sch Vet Med, Retinal Dis Studies Facil, Kennett Sq, PA 19348 USA. EM shelbyr@vet.upenn.edu NR 22 TC 5 Z9 5 U1 0 U2 5 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD JUL 15 PY 2010 VL 237 IS 2 BP 196 EP 199 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 624FH UT WOS:000279801600028 PM 20632794 ER PT J AU Chappell, A Van Pelt, S Zobeck, T Dong, ZB AF Chappell, Adrian Van Pelt, Scott Zobeck, Ted Dong, Zhibao TI Estimating aerodynamic resistance of rough surfaces using angular reflectance SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Dust emission model; Wind erosion; Sheltering; Erodible; Flow separation; Drag; Wake; Aerodynamic resistance; Aerodynamic roughness length; Shadow; Illumination; Ray-casting; Digital elevation model; Roughness density; Frontal area index; Angular reflectance; Bi-directional reflectance ID SOIL SPECTRAL REFLECTANCE; ATMOSPHERIC DUST CYCLE; BIDIRECTIONAL REFLECTANCE; DRAG PARTITION; MODEL; WIND; ELEMENTS; DESERT; ERODIBILITY; EMISSION AB Current wind erosion and dust emission models neglect the heterogeneous nature of surface roughness and its geometric anisotropic effect on aerodynamic resistance, and over-estimate the erodible area by assuming it is not covered by roughness elements. We address these shortfalls with a new model which estimates aerodynamic roughness length (z(0)) using angular reflectance of a rough surface. The new model is proportional to the frontal area index, directional, and represents the geometric anisotropy of z(0). The model explained most of the variation in two sets of wind tunnel measurements of aerodynamic roughness lengths (z(0)). Field estimates of z(0) for varying wind directions were similar to predictions made by the new model. The model was used to estimate the erodible area exposed to abrasion by saltating particles. Vertically integrated horizontal flux (F(h)) was calculated using the area not covered by non-erodible hemispheres; the approach embodied in dust emission models. Under the same model conditions, F(h) estimated using the new model was up to 85% smaller than that using the conventional area not covered. These F(h) simulations imply that wind erosion and dust emission models without geometric anisotropic sheltering of the surface, may considerably over-estimate F(h) and hence the amount of dust emission. The new model provides a straightforward method to estimate aerodynamic resistance with the potential to improve the accuracy of wind erosion and dust emission models, a measure that can be retrieved using bi-directional reflectance models from angular satellite sensors, and an alternative to notoriously unreliable field estimates of z(0) and their extrapolations across landform scales. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved. C1 [Chappell, Adrian] CSIRO Land & Water, Canberra, ACT 2601, Australia. [Van Pelt, Scott] ARS, USDA, Big Spring, TX 79720 USA. [Zobeck, Ted] ARS, USDA, Lubbock, TX 79720 USA. [Dong, Zhibao] Chinese Acad Sci, Key Lab Desert & Desertificat, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu Province, Peoples R China. RP Chappell, A (reprint author), CSIRO Land & Water, GPO Box 1666, Canberra, ACT 2601, Australia. EM adrian.chappell@csiro.au RI Chappell, Adrian/A-7058-2011; CSIRO, SAF/H-3134-2013 OI Chappell, Adrian/0000-0002-0694-7348; NR 41 TC 16 Z9 19 U1 3 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUL 15 PY 2010 VL 114 IS 7 BP 1462 EP 1470 DI 10.1016/j.rse.2010.01.025 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 598ZI UT WOS:000277878900011 ER PT J AU Schiller, I Vordermeier, HM Waters, WR Whelan, AO Coad, M Gormley, E Buddlee, BM Palmer, M Thacker, T McNair, J Welsh, M Hewinson, RG Oesch, B AF Schiller, Irene Vordermeier, H. Martin Waters, W. Ray Whelan, Adam O. Coad, Michael Gormley, Eamonn Buddlee, Bryce M. Palmer, Mitchell Thacker, Tyler McNair, Jim Welsh, Michael Hewinson, R. Glyn Oesch, Bruno TI Bovine tuberculosis: Effect of the tuberculin skin test on in vitro interferon gamma responses SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Review DE Interferon gamma assay; Tuberculin skin test; Caudal fold test; Comparative cervical test; Bovine tuberculosis; Cattle ID MYCOBACTERIUM-BOVIS; IMMUNE-RESPONSES; BLOOD CULTURE; CATTLE; ASSAY; DIAGNOSIS; INFECTION AB Bovine tuberculosis (bTB) is a disease of zoonotic and economic importance. In many countries, control is based on test and slaughter policies and/or abattoir surveillance. For testing, cell mediated immune- (CMI-) based assays (i.e., tuberculin skin test (TST) supplemented by the interferon gamma (IFN-gamma) assay) are the primary surveillance and disease control tests for bTB. The combined use of the in vivo and in vitro CM! assays to increase overall sensitivity has raised the question of whether the IFN-gamma response is influenced by injection of purified protein derivatives (PPDs) for TST. Published data on the influence of the TST, applied as the caudal fold test (CFT) or the comparative cervical test (CCT), on the IFN-gamma assay are contradictory. Reviewing published data and including additional data, the following conclusions can be drawn: (1) in naturally infected cattle, PPD administration for the single or repeated short-interval CCT neither boosts nor depresses PPD-specific IFN-gamma production. Disparate results have been concluded from some studies using experimental infections, emphasizing the importance of confirming initial experimental-based findings with studies using cattle naturally infected with Mycobacterium bovis. (2) In cattle experimentally infected with M. bovis, PPD administration for CFT boosts PPD-specific IFN-gamma production for up to 7 days without any effect on test interpretation. Importantly, in naturally infected cattle, CFT-related boosting selectively increases the in vitro M. bovis PPD (PPD-B) response 3 days after CFT, resulting in an increased PPD-B response relative to the response to Mycobacterium avium PPD (PPD-A). In non-infected cattle, it cannot be excluded that the CFT induces a mild boost of the PPD-specific response, particularly in animals sensitized to environmental, non-tuberculous mycobacteria, thus decreasing the specificity of the IFN-gamma assay. (3) In general, there is a lack of data clearly characterizing the effect of TSTs on the IFN-gamma assay. Further studies are required to clearly describe the effects of both CFT and CCT in non-infected animals and in naturally infected cattle, especially in low reacting infected cattle. (C) 2010 Elsevier B.V. All rights reserved. C1 [Schiller, Irene] Fed Vet Off, CH-3003 Bern, Switzerland. [Vordermeier, H. Martin; Whelan, Adam O.; Coad, Michael; Hewinson, R. Glyn] Vet Lab Agcy, Addlestone, Surrey, England. [Waters, W. Ray; Palmer, Mitchell; Thacker, Tyler] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Gormley, Eamonn] Univ Coll Dublin, Dublin 2, Ireland. [Buddlee, Bryce M.] AgResearch, Palmerston North, New Zealand. [McNair, Jim; Welsh, Michael] AFBI Vet Sci Div, Stormont, Ireland. [Oesch, Bruno] Malcisbo AG, Zurich, Switzerland. RP Schiller, I (reprint author), Fed Vet Off, Schwarzenburgstr 155, CH-3003 Bern, Switzerland. EM irene.schiller@bvet.admin.ch RI Hewinson, Glyn/F-7077-2010; Coad, Mick/D-8093-2011; Whelan, Adam/C-9497-2011; Hewinson, Glyn/J-1902-2014; Vordermeier, H Martin/C-6936-2011; APHA, Staff publications/E-6082-2010; OI Gormley, Eamonn/0000-0003-3900-0393; Thacker, Tyler/0000-0001-6779-7649 FU Department for the Environment, Food and Rural Affairs UK (Defra) FX We thank Jessica Pollock, Rachel Huegel, Bart Olthof, and Mike Howard for excellent technical support. We would also like to express our appreciation to the staff of the Animal Service Unit at the VLA and the NADC. Part of this study was funded by the Department for the Environment, Food and Rural Affairs UK (Defra). NR 35 TC 21 Z9 23 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL 15 PY 2010 VL 136 IS 1-2 BP 1 EP 11 DI 10.1016/j.vetimm.2010.02.007 PG 11 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA 624JZ UT WOS:000279815900001 PM 20219253 ER PT J AU Fach, SJ Olivier, A Gallup, JM Waters, TE Ackermann, MR Lehmkuhl, HD Sacco, RE AF Fach, Sasha J. Olivier, Alicia Gallup, Jack M. Waters, Theresa E. Ackermann, Mark R. Lehmkuhl, Howard D. Sacco, Randy E. TI Differential expression of cytokine transcripts in neonatal and adult ovine alveolar macrophages in response to respiratory syncytial virus or toll-like receptor ligation SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Respiratory syncytial virus; Toll-like receptors; Alveolar macrophage; Ovine lung; Neonates ID SMOOTH-MUSCLE-CELLS; IMMUNE GENES; IN-VITRO; INFECTION; INNATE; REPLICATION; CHILDREN; INFANTS; IL-8; EPITHELIA AB Alveolar macrophages (AM phi s) secrete regulatory molecules that are believed to be critical in maintaining normal lung homeostasis. However, in response to activating signals, AM phi s have been shown to become highly phagocytic cells capable of secreting significant levels of pro-inflammatory cytokines. There is evidence to suggest that susceptibility of M phi subpopulations to viral infection, and their subsequent cytokine/chemokine response, is dependent on age of the host. In the present study, we compared bovine respiratory syncytial virus (BRSV) replication and induction of cytokine responses in neonatal ovine AM phi s to those cells isolated from adult animals. While neonatal AM phi s could be infected with BRSV, viral replication was limited as previously shown for AM phi s from mature animals. Interestingly, following BRSV infection, peak mRNA levels of IL-1 beta and IL-8 in neonatal AM phi were several fold higher than levels induced in adult AM phi s. In addition, peak mRNA expression for the cytokines examined occurred at earlier time points in neonatal AM phi s compared to adult AM phi s. However, the data indicated that viral replication was not required for the induction of specific cytokines in either neonatal or adult AM phi s. TLR3 and TLR4 agonists induced significantly higher levels of cytokine transcripts than BRSV in both neonatal and adult AM phi s. It was recently proposed that immaturity of the neonatal immune system extends from production of pro-inflammatory cytokines to regulation of such responses. Differential regulation of cytokines in neonatal AM phi s compared to adult AM phi s in response to RSV could be a contributory factor to more severe clinical episodes seen in neonates. Published by Elsevier B.V. C1 [Fach, Sasha J.; Waters, Theresa E.; Lehmkuhl, Howard D.; Sacco, Randy E.] ARS, Resp Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Fach, Sasha J.; Sacco, Randy E.] Iowa State Univ, Immunobiol Grad Program, Ames, IA USA. [Olivier, Alicia; Gallup, Jack M.; Ackermann, Mark R.] Iowa State Univ, Dept Vet Pathol, Ames, IA USA. RP Sacco, RE (reprint author), ARS, Resp Dis Res Unit, Natl Anim Dis Ctr, USDA, 2300 Dayton Ave, Ames, IA 50010 USA. EM randy.sacco@ars.usda.gov FU NIH [R01 AI062787] FX This work was supported in part by NIH R01 AI062787. Dr. David Meyerholz, LeaAnn Hobbs, Margie Carter, Jim Fosse, and Kim Driftmier are greatly appreciated for their intellectual input and excellent technical assistance. NR 38 TC 11 Z9 11 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL 15 PY 2010 VL 136 IS 1-2 BP 55 EP 64 DI 10.1016/j.vetimm.2010.02.008 PG 10 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA 624JZ UT WOS:000279815900006 PM 20207014 ER PT J AU Nerren, JR He, HQ Genovese, K Kogut, MH AF Nerren, Jessica R. He, Haiqi Genovese, Kenneth Kogut, Michael H. TI Expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by Gram-negative and Gram-positive bacteria, but not TLR agonists SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Heterophils; Toll-like receptor; Bacteria; Innate immunity; Chicken ID ENTERICA SEROVAR TYPHIMURIUM; COMMERCIAL BROILER-CHICKENS; FUNCTIONAL-CHARACTERIZATION; SALMONELLA-ENTERITIDIS; RHODOCOCCUS-EQUI; GENE-EXPRESSION; IDENTIFICATION; INFECTION; PHAGOCYTOSIS; CELLS AB Toll-like receptors (TLRs) are a critical component of the innate immune response of mammalian and avian species. While most mammalian TLRs have been well characterized, the chicken-specific TLR15 has not been extensively studied. We recently demonstrated that TLR15 is differentially expressed between Salmonella-susceptible-and-resistant chickens, indicating a potential role in the innate immune response to infection with Salmonella. The aim of the present study was to gain better insight into the nature of the ligand for TLR15 by characterizing gene expression patterns of TLR15 by heterophils in response to numerous bacterial-derived TLR agonists LPS, flagellin, CpG oligodeoxynucleotides, lipotechoic acid (LTA), peptidoglycan (PGN), and Pam3CSK4 (PAM), stimulation with live Salmonella enterica serovar Enteritidis (SE-used as a positive control), chicken isolates of Escherichia coli (EC) and Enterococcus gallinarum (EG), the equine-specific pathogen Rhodococcus equi, and stimulation with heat-killed, and formalin-killed SE, EC, and EG. TLR15 expression increased significantly in response to stimulation with live, heat-killed and formalin-killed SE, EC, and EG, but was unaffected by stimulation with known TLR agonists and R. equi. Overall, these observations demonstrate that the individual TLR agonists are not the ligand for TLR15, and that TLR15 recognizes a unique, non-secreted, heat-stabile component of both Gram-negative and Gram-positive bacteria commonly found in and/or capable of causing disease in chickens. Published by Elsevier B.V. C1 [Nerren, Jessica R.; He, Haiqi; Genovese, Kenneth; Kogut, Michael H.] ARS, USDA, So Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Nerren, JR (reprint author), ARS, USDA, So Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM jessierae_3@hotmail.com NR 34 TC 33 Z9 40 U1 2 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL 15 PY 2010 VL 136 IS 1-2 BP 151 EP 156 DI 10.1016/j.vetimm.2010.02.017 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA 624JZ UT WOS:000279815900019 PM 20303182 ER PT J AU Boyd, P Hudgens, E Loftus, JP Tompkins, D Wysocki, M Kakach, LT LaBresh, J Baldwin, CL Lunney, JK AF Boyd, Patricia Hudgens, Edward Loftus, John P. Tompkins, Dannielle Wysocki, Michal Kakach, Laura T. LaBresh, Joanna Baldwin, Cynthia L. Lunney, Joan K. TI Expressed gene sequence and bioactivity of the IFN gamma-response chemokine CXCL11 of swine and cattle SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE CXCL11; Chemokines; T cells; Ruminant; Swine; Th1; Chemotaxis ID CELL ALPHA-CHEMOATTRACTANT; T-LYMPHOCYTES; CXCR3; INHIBITION; TOOL AB This report describes the cloning and characterization of expressed gene sequences of the swine and bovine interferon-gamma inducible chemokine CXCL11, or I-TAC, associated with type 1 T-helper immune responses, and affirmation of bioactivity of their yeast-expressed protein products. The coding regions of both cDNA sequences were 303 nucleotides in length: each is coded for four exons in the genome. The bovine coding region shared 82% and 70% homology with human and mouse CXCL11 respectively, and the swine coding region 84% and 72% homology, respectively. As expected the swine and bovine CXCL11 sequences showed less homology with other human and mouse C-X-C motif chemokine sequences. Each cDNA was cloned into plasmids and transfected into Pichia pastoris (yeast) and the resultant expressed protein purified. Biological activity of each purified chemokine was affirmed by chemotaxis assays. Both swine and bovine CXCL11 were chemotactic for mitogen and IL-2 stimulated peripheral blood mononuclear cells. This is the first report for bioactivity of this chemokine in livestock species. This work provides valuable new reagents for investigating basic immunity as well as vaccine and disease responses in swine and cattle, goals of the U.S. Veterinary Immune Reagent Network which supported this effort. Published by Elsevier B.V. C1 [Boyd, Patricia; Wysocki, Michal; Lunney, Joan K.] ARS, Anim Parasit Dis Lab, ANRI, USDA, Beltsville, MD 20705 USA. [Hudgens, Edward; Loftus, John P.; Tompkins, Dannielle; Baldwin, Cynthia L.] Univ Massachusetts, Dept Vet & Anim Sci, US Vet Immune Reagent Network, Amherst, MA 01003 USA. [Kakach, Laura T.; LaBresh, Joanna] Kingfisher Biotech Inc, St Paul, MN 55114 USA. RP Lunney, JK (reprint author), ARS, Anim Parasit Dis Lab, ANRI, USDA, Bldg 1040,Room 103,BARC E, Beltsville, MD 20705 USA. EM Joan.Lunney@ars.usda.gov FU USDA [2006-35204-16880] FX We thank Vanessa Mailloux and Carolyn Herzig, Univ. MA, for technical assistance with the preparation of cells, and Assiatu Crossman, USDA ARS BARC, for assistance in data presentation. Funding for this work was provided by USDA NIFA competitive grants program grant #2006-35204-16880 for the "US Veterinary Immune Reagent Network" (www.vetimm.org). NR 23 TC 4 Z9 5 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL 15 PY 2010 VL 136 IS 1-2 BP 170 EP 175 DI 10.1016/j.vetimm.2010.02.011 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA 624JZ UT WOS:000279815900022 PM 20347492 ER PT J AU Yeh, HY Klesius, PH AF Yeh, Hung-Yueh Klesius, Phillip H. TI Identification, phylogenetic relationships, characterization and gene expression patterns of six different annexins of channel catfish (Ictalurus punctatus Rafinesque, 1818) SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Annexins; Channel catfish; Ictalurus punctatus; Edwardsiella ictaluri ID ISOTHERMAL AMPLIFICATION METHOD; ACTIVATED CHLORIDE CONDUCTANCE; EDWARDSIELLA-ICTALURI; MOLECULAR-CLONING; RAPID DETECTION; PROTEIN; A5; DYNAMICS; IV; VI AB Annexins are Ca(2+)-dependent phospholipid-binding proteins. They are ubiquitous in living organisms and are involved in many cellular processes. In the course of studying Edwardsiella ictaluri pathogenesis in channel catfish, we identified that six annexin expressed sequence tags (A1, A2, A4, A5, A6 and A11) were up-regulated at the early stage of infection. In this study, we cloned and characterized these transcripts. The full-length nucleic acid sequences of channel catfish annexins ranges from 1231 (annexin A1) to 2476 (annexin A6). Each transcript has one open reading, which appears to encode peptides ranges from 317 to 662 amino acid residues with the calculated molecular masses from 35.0 (annexin A5) to 74.5 kDa (annexin A6). Phylogenetic and sequence analyses demonstrate that each channel catfish annexin had a diversified amino terminus, and had four structurally conserved 70-amino acid repeats. In addition, several important features for annexin functions were conserved in channel catfish. For expression profile, channel catfish annexin A1, A4 and A6 transcripts were detected in spleen, anterior kidney, liver, intestine, skin and gill of fish examined. However, annexin A2, A5 and A11 cDNAs were variously detected in tissues of fish sampled. This result provides important information for further elucidating channel catfish annexin functions in vivo. Published by Elsevier B.V. C1 [Yeh, Hung-Yueh; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Yeh, HY (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 900 Wire Rd, Auburn, AL 36832 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service [6420-32000-024-00D] FX This study was supported by the USDA Agricultural Research Service CRIS project no. 6420-32000-024-00D. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 42 TC 6 Z9 6 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL 15 PY 2010 VL 136 IS 1-2 BP 176 EP 183 DI 10.1016/j.vetimm.2010.02.013 PG 8 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA 624JZ UT WOS:000279815900023 PM 20227769 ER PT J AU Schares, G Basso, W Majzoub, M Rostaher, A Scharr, JC Langenmayer, MC Selmair, J Dubey, JP Cortes, HC Conraths, FJ Gollnick, NS AF Schares, G. Basso, W. Majzoub, M. Rostaher, A. Scharr, J. C. Langenmayer, M. C. Selmair, J. Dubey, J. P. Cortes, H. C. Conraths, F. J. Gollnick, N. S. TI Comparative evaluation of immunofluorescent antibody and new immunoblot tests for the specific detection of antibodies against Besnoitia besnoiti tachyzoites and bradyzoites in bovine sera SO VETERINARY PARASITOLOGY LA English DT Article DE Besnoitiosis; Besnoitia besnoiti; Neospora caninum; Tachyzoite; Bradyzoite; Immuno blot; Immunofluorescent antibody test; Cattle ID NEOSPORA-CANINUM; TOXOPLASMA-GONDII; INFECTED CATTLE; TRANSMISSION; ELISA AB Besnoitia besnoiti, an apicomplexan parasite causes economically important disease in cattle in many countries of Africa and Asia is re-emerging in Europe. Serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. We report new, simplified immunoblot-based serological tests for the detection of B. besnoiti-specific antibodies. Antigens were used under non-reducing conditions in the immunoblots, because reduction of the antigen with beta-mercaptoethanol diminished the antigenicity in both, tachyzoites and bradyzoites. Ten B. besnoiti tachyzoite and ten bradyzoite antigens of 15-45 kDa molecular weight were recognized by B. besnoiti infected cattle, but not or only weakly detected by cattle infected with related protozoan parasites, Neospora caninum, Toxoplasma gondii, Sarcocystis cruzi, Sarcocystis hominis, or Sarcocystis hirsuta. The sensitivity and specificity of B. besnoiti immunoblots were determined with sera from 62 German cattle with clinically confirmed besnoitiosis and 404 sera from unexposed German cattle including 214 sera from animals with a N. caninum-specific antibody response. Using a new scoring system, the highest specificity (100%) and sensitivity (90%) of the immunoblots were observed when reactivity to at least four of the ten selected tachyzoite or bradyzoite antigens was considered as positive. When a cut-off based on this scoring system was applied to both the tachyzoite- and the bradyzoite-based immunoblots, there was an almost perfect agreement with the indirect fluorescent antibody test with a titre of 200 as the positive cut-off. We identified and partially characterized 10 tachyzoite and 10 bradyzoite B. besnoiti antigens which may help to develop new specific and sensitive serological tests based on individual antigens and in the identification of possible vaccine candidates. (C) 2010 Elsevier B.V. All rights reserved. C1 [Schares, G.; Basso, W.; Conraths, F. J.] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, D-16868 Wusterhausen, Germany. [Basso, W.] Univ Nacl La Plata, Fac Ciencias Vet, Lab Inmunoparasitol, La Plata, Buenos Aires, Argentina. [Basso, W.] Consejo Nacl Invest Cient & Tecn, CONICET, Buenos Aires, DF, Argentina. [Majzoub, M.] Univ Munich, Inst Vet Pathol, Munich, Germany. [Rostaher, A.] Univ Munich, Clin Small Anim Med, Munich, Germany. [Scharr, J. C.; Langenmayer, M. C.; Gollnick, N. S.] Univ Munich, Clin Ruminants Ambulatory & Herd Hlth Serv, Munich, Germany. [Dubey, J. P.] ARS, USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville, MD USA. [Cortes, H. C.] Univ Evora, Lab Parasitol Victor Caeiro, ICAAM, Evora, Portugal. RP Schares, G (reprint author), Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, Seestr 55, D-16868 Wusterhausen, Germany. EM gereon.schares@fli.bund.de OI Cortes, Helder/0000-0003-4644-2165; Schares, Gereon/0000-0002-3217-289X; Conraths, Franz/0000-0002-7400-9409 FU Alexander von Humboldt Foundation, Bonn, Germany FX We thank Lieselotte Minke, Andrea Barwald, Anika Ruppert and Aline Beckert for their excellent technical assistance. W. Basso was supported by a Georg Forster Research Fellowship of the Alexander von Humboldt Foundation, 53173 Bonn, Germany. NR 22 TC 40 Z9 41 U1 0 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 15 PY 2010 VL 171 IS 1-2 BP 32 EP 40 DI 10.1016/j.vetpar.2010.03.017 PG 9 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 622EP UT WOS:000279644100005 PM 20378250 ER PT J AU Li, Q Li, L Yang, XH Warburton, ML Bai, GH Dai, JR Li, JS Yan, JB AF Li, Qing Li, Lin Yang, Xiaohong Warburton, Marilyn L. Bai, Guanghong Dai, Jingrui Li, Jiansheng Yan, Jianbing TI Relationship, evolutionary fate and function of two maize co-orthologs of rice GW2 associated with kernel size and weight SO BMC PLANT BIOLOGY LA English DT Article ID QUANTITATIVE TRAIT LOCI; MULTILOCUS GENOTYPE DATA; E3 UBIQUITIN LIGASE; GRAIN-YIELD; SEQUENCE ALIGNMENT; DIFFERENT TESTERS; NATURAL VARIATION; DUPLICATE GENES; DRAFT SEQUENCE; SEED SIZE AB Background: In rice, the GW2 gene, found on chromosome 2, controls grain width and weight. Two homologs of this gene, ZmGW2-CHR4 and ZmGW2-CHR5, have been found in maize. In this study, we investigated the relationship, evolutionary fate and putative function of these two maize genes. Results: The two genes are located on duplicated maize chromosomal regions that show co-orthologous relationships with the rice region containing GW2. ZmGW2-CHR5 is more closely related to the sorghum counterpart than to ZmGW2-CHR4. Sequence comparisons between the two genes in eight diverse maize inbred lines revealed that the functional protein domain of both genes is completely conserved, with no non-synonymous polymorphisms identified. This suggests that both genes may have conserved functions, a hypothesis that was further confirmed through linkage, association, and expression analyses. Linkage analysis showed that ZmGW2-CHR4 is located within a consistent quantitative trait locus (QTL) for one-hundred kernel weight (HKW). Association analysis with a diverse panel of 121 maize inbred lines identified one single nucleotide polymorphism (SNP) in the promoter region of ZmGW2-CHR4 that was significantly associated with kernel width (KW) and HKW across all three field experiments examined in this study. SNPs or insertion/deletion polymorphisms (InDels) in other regions of ZmGW2-CHR4 and ZmGW2-CHR5 were also found to be significantly associated with at least one of the four yield-related traits (kernel length (KL), kernel thickness (KT), KW and HKW). None of the polymorphisms in either maize gene are similar to each other or to the 1 bp InDel causing phenotypic variation in rice. Expression levels of both maize genes vary over ear and kernel developmental stages, and the expression level of ZmGW2-CHR4 is significantly negatively correlated with KW. Conclusions: The sequence, linkage, association and expression analyses collectively showed that the two maize genes represent chromosomal duplicates, both of which function to control some of the phenotypic variation for kernel size and weight in maize, as does their counterpart in rice. However, the different polymorphisms identified in the two maize genes and in the rice gene indicate that they may cause phenotypic variation through different mechanisms. C1 [Li, Qing; Li, Lin; Yang, Xiaohong; Bai, Guanghong; Dai, Jingrui; Li, Jiansheng; Yan, Jianbing] China Agr Univ, Minist Agr, Key Lab Crop Genom & Genet Improvement, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China. [Warburton, Marilyn L.] USDA ARS Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Bai, Guanghong] Xinjiang Agr Univ, Coll Agr, Urumqi 830052, Xinjiang, Peoples R China. [Yan, Jianbing] CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico. RP Li, JS (reprint author), China Agr Univ, Minist Agr, Key Lab Crop Genom & Genet Improvement, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China. EM lijiansheng@cau.edu.cn; yjianbing@gmail.com FU National Hi-Tech Research and Development Program of China [2006AA10Z183, 2006AA10A107]; National Basic Research and Development Program of China [2007CB10900] FX This research was supported by the National Hi-Tech Research and Development Program of China (2006AA10Z183, 2006AA10A107) and National Basic Research and Development Program of China (2007CB10900). Thanks would be given to Dr. Jihua Tang, who is a previous member in our lab and now works in Henan Agricultural University, for sharing the IF2 design and relevant data. NR 73 TC 43 Z9 55 U1 7 U2 47 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUL 14 PY 2010 VL 10 AR 143 DI 10.1186/1471-2229-10-143 PG 15 WC Plant Sciences SC Plant Sciences GA 637TJ UT WOS:000280841300001 PM 20626916 ER PT J AU McKeown, NM Jacobs, DR AF McKeown, Nicola M. Jacobs, David R., Jr. TI In defence of phytochemical-rich dietary patterns SO BRITISH JOURNAL OF NUTRITION LA English DT Editorial Material ID CORONARY-HEART-DISEASE; WHOLE-GRAIN; RISK-FACTORS; CARDIOVASCULAR-DISEASE; INSULIN SENSITIVITY; MEDITERRANEAN DIET; LIPID-PEROXIDATION; METABOLIC SYNDROME; RANDOMIZED-TRIAL; MEN C1 [McKeown, Nicola M.] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. [Jacobs, David R., Jr.] Univ Minnesota, Div Epidemiol & Community Hlth, Sch Publ Hlth, Minneapolis, MN 55454 USA. RP McKeown, NM (reprint author), Tufts Univ, JM USDA HNRCA, 711 Washington St, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu NR 25 TC 11 Z9 11 U1 1 U2 2 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 J9 BRIT J NUTR JI Br. J. Nutr. PD JUL 14 PY 2010 VL 104 IS 1 BP 1 EP 3 DI 10.1017/S0007114510000656 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 623NM UT WOS:000279748400001 PM 20236555 ER PT J AU Sauer, J Jang, H Zimmerly, EM Kim, KC Liu, ZH Chanson, A Smith, DE Mason, JB Friso, S Choi, SW AF Sauer, Julia Jang, Hyeran Zimmerly, Ella M. Kim, Kyong-chol Liu, Zhenhua Chanson, Aurelie Smith, Donald E. Mason, Joel B. Friso, Simonetta Choi, Sang-Woon TI Ageing, chronic alcohol consumption and folate are determinants of genomic DNA methylation, p16 promoter methylation and the expression of p16 in the mouse colon SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Alcohol; DNA methylation; p16; Folate; Ageing; Colon ID RAT COLON; CANCER; ETHANOL; HYPOMETHYLATION; CARCINOGENESIS; METABOLISM; GENE; AGE; TUMORIGENESIS; NUTRITION AB Older age, dietary folate and chronic alcohol consumption are important risk factors for the development of colon cancer. The present study examined the effects of ageing, folate and alcohol on genomic and p16-specific DNA methylation, and p16 expression in the murine colon. Old (aged 18 months; n 70) and young (aged 4 months; n 70) male C57BL/6 mice were pair-fed either a Lieber-DeCarli liquid diet with alcohol (18% of energy), a Lieber-DeCarli diet with alcohol (18 %) and reduced folate (0.25 mg folate/l) or an isoenergetic control diet (0.5 mg folate/l) for 5 or 10 weeks. Genomic DNA methylation, p16 promoter methylation and p16 gene expression were analysed by liquid chromatography-MS, methylation-specific PCR and real-time RT-PCR, respectively. Genomic DNA methylation was lower in the colon of old mice compared with young mice (P<0.02) at 10 weeks. Alcohol consumption did not alter genomic DNA methylation in the old mouse colon, whereas it tended to decrease genomic DNA methylation in young mice (P=0.08). p16 Promoter methylation and expression were higher in the old mouse colon compared with the corresponding young groups. There was a positive correlation between p16 promoter methylation and p16 expression in the old mouse colon (P<0.02). In young mice the combination of alcohol and reduced dietary folate led to significantly decreased p16 expression compared with the control group (P<0.02). In conclusion, ageing and chronic alcohol consumption alter genomic DNA methylation, p16 promoter methylation and p16 gene expression in the mouse colon, and dietary folate availability can further modify the relationship with alcohol in the young mouse. C1 [Sauer, Julia; Jang, Hyeran; Zimmerly, Ella M.; Kim, Kyong-chol; Liu, Zhenhua; Chanson, Aurelie; Mason, Joel B.; Choi, Sang-Woon] Tufts Univ, Vitamins & Carcinogenesis Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Kim, Kyong-chol] Mizmedi Hosp, Dept Family Med, Seoul, South Korea. [Smith, Donald E.] Tufts Univ, Comparat Biol Unit, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Friso, Simonetta] Univ Verona, Sch Med, I-37100 Verona, Italy. RP Choi, SW (reprint author), Tufts Univ, Vitamins & Carcinogenesis Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM sang.choi@tufts.edu FU United States Department of Agriculture [581950-9-001]; National Institutes of Health [R21 AA016681, R01 AG025834, U54 CA 10097, K05 CA100048] FX This material is based upon work supported by the United States Department of Agriculture, under agreement no. 581950-9-001. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the United States Department of Agriculture.; This project has been supported in part by the National Institutes of Health grants R21 AA016681 and R01 AG025834 (to S.-W. C.) and the National Institutes of Health grants U54 CA 10097 and K05 CA100048 (to J. B. M.). NR 44 TC 16 Z9 16 U1 2 U2 8 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 J9 BRIT J NUTR JI Br. J. Nutr. PD JUL 14 PY 2010 VL 104 IS 1 BP 24 EP 30 DI 10.1017/S0007114510000322 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 623NM UT WOS:000279748400005 PM 20205967 ER PT J AU Choi, SH Lee, SH Kim, HJ Lee, IS Kozukue, N Levin, CE Friedman, M AF Choi, Suk-Hyun Lee, Sang-Hwa Kim, Hyun-Jeong Lee, In-Seon Kozukue, Nobuyuki Levin, Carol E. Friedman, Mendel TI Changes in Free Amino Acid, Phenolic, Chlorophyll, Carotenoid, and Glycoalkaloid Contents in Tomatoes during 11 Stages of Growth and Inhibition of Cervical and Lung Human Cancer Cells by Green Tomato Extracts SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Tomato; free amino acids; phenolic compounds; chlorophylls; carotenoids; glycoalkaloids; cancer ID POTATO IPOMOEA-BATATAS; LIQUID-CHROMATOGRAPHY; BETA-CAROTENE; LYCOPENE; DEHYDROTOMATINE; TOXICITY; FRUIT; COLON; LIVER; PLANT AB Tomato (Solanum lycopersicum) plants synthesize nutrients, pigments, and secondary metabolites that benefit nutrition and human health. The concentrations of these compounds are strongly influenced by the maturity of the tomato fruit on the vine. Widely consumed Korean tomatoes of the variety Doturakworld were analyzed for changes in the content of free amino acids, phenolic compounds, chlorophylls, carotenoids, and glycoalkaloids at 11 stages (S1-S11) of ripeness. The results show that (a) the total content (in mg/100 g of FW) of the free amino acids and other nitrogen-containing compounds in the extracts ranged from about 41 to 85 in the green tomato extracts S1-S7 and then increased to 251 (S9) in the red extracts, followed by a decrease to 124 in S11 red extracts; (b) the total initial concentration and composition of up to 12 phenolic compounds of similar to 2000 mu g/100 g of FW varied throughout the ripening process, with the quantity decreasing and the number of individual compounds increasing in the red tomato; (c) chlorophyll a and b content of tomatoes harvested during Si was 5.73 mg/100 g of fresh pericarp and then decreased continuously to 1.14 mg/100 g for S11; (d) the concentration (in mg/100 g of RN) of lycopene in the S8 red extract of 0.32 increased to 1.27 in S11; and (e) tomatoes harvested during Si contained 48.2 mg of dehydrotomatine/100 g of FW, and this value continually decreased to 1.5 in S7, with no detectable levels in S8-S11. The corresponding alpha-tomatine content decreased from S1 (361) to S8 (13.8). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell assay IC(50) values showed that Hel299 lung cells, A549 lung cancer cells, and HeLa cervical carcinoma cells were highly susceptible to inactivation by glycoalkaloid-rich green tomato extracts. Chang normal liver cells and U937 lymphoma cells were less susceptible. The possible significance of the results for plant physiology and the diet is discussed. C1 [Levin, Carol E.; Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Choi, Suk-Hyun] Seowon Univ, Dept Food Serv Ind, Cheongju 361742, South Korea. [Lee, Sang-Hwa] Seowon Univ, Dept Food Nutr, Cheongju 361742, South Korea. [Kozukue, Nobuyuki] Seowon Univ, Bio Organ Mat & Food Ctr, Cheongju 361742, South Korea. [Kim, Hyun-Jeong; Lee, In-Seon] Keimyung Univ, Ctr Tradit Microorganism Resources, Taegu 704701, South Korea. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM mendel.friedman@ars.usda.gov OI Levin, Carol/0000-0001-6522-6156 NR 54 TC 20 Z9 21 U1 6 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 7547 EP 7556 DI 10.1021/jf100162j PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900009 PM 20560602 ER PT J AU White, BL Howard, LR Prior, RL AF White, Brittany L. Howard, Luke R. Prior, Ronald L. TI Release of Bound Procyanidins from Cranberry Pomace by Alkaline Hydrolysis SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Alkaline; cranberry; hydrolysis; pomace; procyanidins; unextractable ID CELL-WALL MATERIAL; BASE-CATALYZED REACTIONS; NONCOVALENT INTERACTION; POLYMERIC PROCYANIDINS; CONDENSED TANNINS; THEOBROMA-CACAO; IN-VITRO; PROANTHOCYANIDINS; QUANTIFICATION; EXTRACTION AB Procyanidins in plant products are present as extractable or unextractable/bound forms. We optimized alkaline hydrolysis conditions to liberate procyanidins and depolymerize polymers from dried cranberry pomace. Alkaline extracts were neutralized (pH 6-7) and then procyanidins were extracted with ethyl acetate and analyzed by normal phase high performance liquid chromatography. Alkaline hydrolysis resulted in an increase in low molecular weight procyanidins, and the increase was greater at higher temperature, short time combinations. The most procyanidins (DP1-DP3) were extracted at 60 degrees C for 15 min with each concentration of NaOH. When compared to conventional extraction using homogenization with acetone/water/acetic acid (70:29.5:0.5 v/v/v), treatment with NaOH increased procyanidin oligomer extraction by 3.8-14.9-fold, with the greatest increase being DP1 (14.9x) and A-type DP2 (8.4x) procyanidins. Alkaline treatment of the residue remaining after conventional extraction resulted in further procyanidin extraction, indicating that procyanidins are not fully extracted by conventional extraction methods. C1 [White, Brittany L.; Howard, Luke R.] Univ Arkansas, Dept Food Sci, Fayetteville, AR 72703 USA. [Prior, Ronald L.] ARS, USDA, Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA. RP Howard, LR (reprint author), Univ Arkansas, Dept Food Sci, 2650 N Young Ave, Fayetteville, AR 72703 USA. EM lukeh@uark.edu NR 32 TC 29 Z9 30 U1 1 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 7572 EP 7579 DI 10.1021/jf100700p PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900012 PM 20527966 ER PT J AU Haas, MJ Adawi, N Berry, WW Feldman, E Kasprzyk, S Ratigan, B Scott, K Landsburg, EB AF Haas, Michael J. Adawi, Nadia Berry, William W. Feldman, Elaine Kasprzyk, Stephen Ratigan, Brian Scott, Karen Landsburg, Emily Bockian TI Butter as a Feedstock for Biodiesel Production SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Biodiesel; butter; fatty acid methyl ester AB Fatty acid methyl esters (FAME) were produced from cow's milk (Bostaurus) butter by esterification/transesterification in the presence of methanol. The product was assayed according to the Standard Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels (ASTM D 6751). The preparation failed to meet the specifications for flash point, free and total glycerin contents, total sulfur, and oxidation stability. Failures to meet the flash point and free/total glycerin specifications were determined to be due to interference with standard assays for these parameters by short-chain-length fatty acid esters. The oxidation stability of the butterfat FAME was improved by supplementation with a commercial antioxidant formulation. Approximately 725 ppm of antioxidant was required to meet the ASTM-specified stability value for biodiesel. This work indicates that, without further purification to reduce a slightly excessive sulfur content, fatty acid ester preparations produced from butter are unacceptable as sole components of a biodiesel fuel. However, it is possible that even without further purification a butter-based ester preparation could be mixed with biodiesel from other feedstocks to produce a blend that meets the current quality standards for biodiesel. The results presented here also illustrate some potential weaknesses in the accepted methods for biodiesel characterization when employed in the analysis of FAME preparations containing mid- and short-chain fatty acid esters. C1 [Haas, Michael J.; Scott, Karen] ARS, Fats Oils & Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Adawi, Nadia; Feldman, Elaine; Kasprzyk, Stephen; Ratigan, Brian; Landsburg, Emily Bockian] BlackGold Biofuels, Philadelphia, PA 19107 USA. [Berry, William W.] Proc Technol Associates, Lakeland, FL 33807 USA. RP Haas, MJ (reprint author), ARS, Fats Oils & Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM michael.haas@ars.usda.gov NR 15 TC 5 Z9 5 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 7680 EP 7684 DI 10.1021/jf1003754 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900025 PM 20552957 ER PT J AU Smith, BM Bean, SR Schober, TJ Tilley, M Herald, TJ Aramouni, F AF Smith, Brennan M. Bean, Scott R. Schober, Tilman J. Tilley, Michael Herald, Thomas J. Aramouni, Fadi TI Composition and Molecular Weight Distribution of Carob Germ Protein Fractions SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Carob germ flour; gluten; celiac disease; gluten-free; protein; light scattering; caroubin ID GLUTEN-LIKE PROTEIN; MONOMERIC PROTEINS; LIGHT-SCATTERING; WHEAT PROTEINS; FLOUR; CHROMATOGRAPHY; QUANTITY; CAROUBIN AB Biochemical properties of carob germ proteins were analyzed using a combination of selective extraction, reversed-phase high-performance liquid chromatography (RP-HPLC), size exclusion chromatography (SEC) coupled with multiangle laser light scattering (SEC-MALS), and electrophoretic analysis. Using a modified Osborne extraction procedure, carob germ flour proteins were found to contain similar to 32% albumin and globulin and similar to 68% glutelin with no prolamins detected. The albumin and globulin fraction was found to contain low amounts of disulfide-bonded polymers with relatively low M(w) ranging up to 5 x 10(6) Da. The glutelin fraction, however, was found to contain large amounts of high molecular weight disulfide-bonded polymers with M(w) up to 8 x 10(7) Da. When extracted under nonreducing conditions and divided into soluble and insoluble proteins as typically done for wheat gluten, carob germ proteins were found to be almost entirely (similar to 95%) in the soluble fraction with only (similar to 5%) in the insoluble fraction. As in wheat, SEC-MALS analysis showed that the insoluble proteins had a greater M(w) than the soluble proteins and ranged up to 8 x 10(7) Da. The lower M(w) distribution of the polymeric proteins of carob germ flour may account for differences in functionality between wheat and carob germ flour. C1 [Bean, Scott R.; Aramouni, Fadi] Kansas State Univ, Inst Food Sci, Manhattan, KS 66506 USA. [Smith, Brennan M.; Schober, Tilman J.; Tilley, Michael; Herald, Thomas J.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. RP Bean, SR (reprint author), Kansas State Univ, Inst Food Sci, Manhattan, KS 66506 USA. EM scott.bean@ars.usda.gov OI Bean, Scott/0000-0001-8678-8094 NR 28 TC 9 Z9 9 U1 2 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 7794 EP 7800 DI 10.1021/jf101523p PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900040 PM 20557053 ER PT J AU Light, DM Beck, JJ AF Light, Douglas M. Beck, John J. TI Characterization of Microencapsulated Pear Ester, (2E,4Z)-Ethyl-2,4-decadienoate, a Kairomonal Spray Adjuvant against Neonate Codling Moth Larvae SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Codling moth; Cydia pomonella; pear ester; ethyl (2E,4Z)-2,4-decadienoate; microcapsules; kairomone; SPME; volatile emission ID CYDIA-POMONELLA L.; CONTROLLED-RELEASE; POME FRUIT; LEPIDOPTERA; TORTRICIDAE; GRANULOVIRUS; FORMULATIONS; DISRUPTION; MICROCAPSULES; PHEROMONE AB Codling moth (CM), Cydia pomonella (Lepidoptera: Tortricidae), is the key pest of apples, pears, and walnuts worldwide. The pear-derived kairomone, ethyl (2E,4Z)-2,4-decadienoate, the pear ester (PE), evokes attraction and arrestment of CM larvae. Microencapsulated PE formulation (PE-MEC) enhances the control efficacy of insecticides when used as a spray adjuvant. Characterization of the microencapsulated kairomone, including microcapsule size, concentrations, emission rates, and larval response, was performed. Microcapsule diameter ranged from 2 to 14 mu m, with 68% of capsules being 2-3 mu m, and the concentration of microcapsules averaged 25.9 x 10(4) capsules per mL of field spray solution. Headspace collections showed emission of PE was related to PE-MEC concentration and was best described as first-order power decay. Neonate larvae responded to PE-MEC applications aged through 14 days. These results demonstrated that application of PE-MEC concurrent with insecticides may increase neonate foliar wandering, thereby disrupting host location and enhancing mortality by prolonging its exposure to insecticide. C1 [Light, Douglas M.; Beck, John J.] ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Light, DM (reprint author), ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM doug.light@ars.usda.gov NR 33 TC 11 Z9 12 U1 2 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 7838 EP 7845 DI 10.1021/jf101167p PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900045 PM 20527813 ER PT J AU Jiang, HX Lio, JY Blanco, M Campbell, M Jane, JL AF Jiang, Hongxin Lio, Junyi Blanco, Mike Campbell, Mark Jane, Jay-Lin TI Resistant-Starch Formation in High-Amylose Maize Starch during Kernel Development SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Resistant starch; high-amylose maize; ae mutant; amylose double helices; amylose-lipid complex; kernel development ID PHYSICOCHEMICAL PROPERTIES; TEMPERATURE TREATMENT; INTERNAL STRUCTURE; LIPID COMPLEXES; PLASMA-GLUCOSE; INSULIN; GELATINIZATION; GRANULE; BARLEY; AMYLOPECTIN AB The objective of this study was to understand the resistant-starch (RS) formation during kernel development of a high-amylose maize, GEMS-0067 line. The RS content of the starch, determined using AOAC method 991.43 for total dietary fiber, increased with kernel maturation and increase in the amylose/intermediate component (IC) content of the starch. Gelatinization of the native starches showed a major thermal transition with peak temperature at 76.6-81.0 degrees C. An additional peak (similar to 97.1 degrees C) first appeared 20 days after pollination and then developed into a significant peak on later dates. After removal of lipids from the starch, this peak disappeared, but the conclusion gelatinization temperature remained the same. The proportion of the enthalpy change of the thermal transition above 95 degrees C, calculated from the thermogram of the defatted starch, increased with kernel maturation and was significantly correlated with the RS content of the starch (r = 0.98). These results showed that the increase in crystallites of amylose/IC long-chain double helices in the starch resulted in the increase in the RS content of the starch during kernel development. C1 [Jiang, Hongxin; Lio, Junyi; Jane, Jay-Lin] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. [Blanco, Mike] ARS, Plant Intro Res Unit, USDA, Ames, IA 50011 USA. [Campbell, Mark] Truman State Univ, Div Sci, Kirksville, MO 63501 USA. RP Jane, JL (reprint author), Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. EM jjane@iastate.edu FU USDA-ARS FX Received for review March 19, 2010. Revised manuscript received May 24, 2010. Accepted June 1, 2010. We thank the USDA-ARS GEM project for support on this research. NR 41 TC 24 Z9 25 U1 0 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 14 PY 2010 VL 58 IS 13 BP 8043 EP 8047 DI 10.1021/jf101056y PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 621JX UT WOS:000279573900071 PM 20550134 ER PT J AU Gailbreath, KL O'Toole, D Taus, NS Knowles, DP Oaks, JL Li, H AF Gailbreath, Katherine L. O'Toole, Donal Taus, Naomi S. Knowles, Donald P. Oaks, J. Lindsay Li, Hong TI Experimental nebulization of American bison (Bison bison) with low doses of ovine herpesvirus 2 from sheep nasal secretions SO VETERINARY MICROBIOLOGY LA English DT Article DE Ovine herpesvirus 2; Malignant catarrhal fever; Bison; Experimental infection ID MALIGNANT CATARRHAL FEVER; CATTLE; PCR; INFECTION; FEEDLOT; ASSAY AB Malignant catarrhal fever (MCF), caused by ovine herpesvirus 2 (OvHV-2), is an important cause of mortality in ranched American bison and domestic cattle in North America. Previous studies showed that bison can be infected by intranasal nebulization with sheep nasal secretions containing OvHV-2 and provided preliminary information on viral doses required for infection and disease progression. The goals of this study were to establish optimal minimal infectious and minimal lethal doses of OvHV-2 by the intranasal route in bison, evaluate the influence of dose on incubation period and other clinical parameters and determine if bison seropositive for antibody against MCF-group viruses are resistant to developing MCF after intranasal challenge. In this study, the minimal infectious dose and minimal lethal dose overlap, suggesting that experimental production of subclinically infected bison is impractical. Dose is inversely related to both incubation period and the period between nebulization and first detection of >1000 OvHV-2 DNA copies/500 ng total DNA in peripheral blood leukocytes. Interestingly, all of the bison seropositive for antiMCF-group viral antibody prior to inoculation died of MCF after nebulization. We conclude that previous exposure to an MCF-group virus does not necessarily provide resistance to OvHV-2-induced MCF in bison. Published by Elsevier B.V. C1 [Gailbreath, Katherine L.; Taus, Naomi S.; Knowles, Donald P.; Li, Hong] Washington State Univ, USDA, ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Gailbreath, Katherine L.; Knowles, Donald P.; Oaks, J. Lindsay] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [O'Toole, Donal] Univ Wyoming, Wyoming State Vet Lab, Laramie, WY 82070 USA. RP Gailbreath, KL (reprint author), Washington State Univ, USDA, ARS, Anim Dis Res Unit, 3003 ADBF, Pullman, WA 99164 USA. EM katherine@vetmed.wsu.edu FU USDA-ARS [CWU 5348-32000-018-00D.] FX We thank Jan Keller, Shirley Elias and Lori Fuller for excellent technical assistance and Rodney Rogers for outstanding animal care. This work was supported by USDA-ARS CWU 5348-32000-018-00D. NR 20 TC 8 Z9 8 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 J9 VET MICROBIOL JI Vet. Microbiol. PD JUL 14 PY 2010 VL 143 IS 2-4 BP 389 EP 393 DI 10.1016/j.vetmic.2009.11.026 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 615HD UT WOS:000279124500038 PM 20018461 ER PT J AU Park, W Scheffler, BE Bauer, PJ Campbell, BT AF Park, Wonkeun Scheffler, Brian E. Bauer, Philip J. Campbell, B. Todd TI Identification of the family of aquaporin genes and their expression in upland cotton (Gossypium hirsutum L.) SO BMC PLANT BIOLOGY LA English DT Article ID MAJOR INTRINSIC PROTEINS; WATER CHANNEL ACTIVITY; PLASMA-MEMBRANE; PLANT AQUAPORINS; ARABIDOPSIS-THALIANA; MAIZE; TONOPLAST; RICE; GENOME; TIP AB Background: Cotton (Gossypium spp.) is produced in over 30 countries and represents the most important natural fiber in the world. One of the primary factors affecting both the quantity and quality of cotton production is water. A major facilitator of water movement through cell membranes of cotton and other plants are the aquaporin proteins. Aquaporin proteins are present as diverse forms in plants, where they function as transport systems for water and other small molecules. The plant aquaporins belong to the large major intrinsic protein (MIP) family. In higher plants, they consist of five subfamilies including plasma membrane intrinsic proteins (PIP), tonoplast intrinsic proteins (TIP), NOD26-like intrinsic proteins (NIP), small basic intrinsic proteins (SIP), and the recently discovered X intrinsic proteins (XIP). Although a great deal is known about aquaporins in plants, very little is known in cotton. Results: From a molecular cloning effort, together with a bioinformatic homology search, 71 upland cotton (G. hirsutum) aquaporin genes were identified. The cotton aquaporins consist of 28 PIP and 23 TIP members with high sequence similarity. We also identified 12 NIP and 7 SIP members that showed more divergence. In addition, one XIP member was identified that formed a distinct 5(th) subfamily. To explore the physiological roles of these aquaporin genes in cotton, expression analyses were performed for a select set of aquaporin genes from each subfamily using semi-quantitative reverse transcription (RT)-PCR. Our results suggest that many cotton aquaporin genes have high sequence similarity and diverse roles as evidenced by analysis of sequences and their expression. Conclusion: This study presents a comprehensive identification of 71 cotton aquaporin genes. Phylogenetic analysis of amino acid sequences divided the large and highly similar multi-gene family into the known 5 aquaporin subfamilies. Together with expression and bioinformatic analyses, our results support the idea that the genes identified in this study represent an important genetic resource providing potential targets to modify the water use properties of cotton. C1 [Park, Wonkeun; Bauer, Philip J.; Campbell, B. Todd] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Scheffler, Brian E.] USDA ARS, MSA Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. RP Campbell, BT (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM todd.campbell@ars.usda.gov OI Scheffler, Brian/0000-0003-1968-8952 FU U.S. Department of Agriculture [6657-21000-005-00D] FX We appreciate Dr. Paxton Payton for providing the RNA isolation protocol. We also appreciate the technical assistance provided by Antoine Barr, Fanny Liu, and Bobby Fisher. The authors would also like to thank Drs. Jonathan Wendel and Martin Wubben for providing excellent comments and suggestions that strengthened this manuscript. This project was supported by funding from CRIS No. 6657-21000-005-00D of the U.S. Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 66 TC 72 Z9 85 U1 2 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUL 13 PY 2010 VL 10 AR 142 DI 10.1186/1471-2229-10-142 PG 17 WC Plant Sciences SC Plant Sciences GA 637TH UT WOS:000280841100001 PM 20626869 ER PT J AU Rango, A Laliberte, AS AF Rango, Albert Laliberte, Andrea S. TI Impact of flight regulations on effective use of unmanned aircraft systems for natural resources applications SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE Small unmanned aircraft systems; FAA regulations; National Airspace System; civilian applications; aerial photography; rangeland management ID AERIAL VEHICLE; IMAGERY AB Unmanned Aircraft Systems (UAS) have great potential for rangeland assessment, monitoring, and numerous other applications in natural resources management. In order for UAS to become a dependable tool for public land management agencies in carrying out their government-mandated responsibilities, it is necessary to integrate UAS into the National Airspace System (NAS), which includes all aircraft, manned or unmanned. To achieve this, Federal Aviation Administration (FAA) regulations have to be followed to assure public safety. UAS operators need to know that FAA safety regulations, which incorporate line-of-sight restrictions, will only allow slow progress towards an operational system, and they must plan accordingly for the extra time necessary to prepare and complete flight missions. By following approved safety procedures, UAS operators can develop a UAS flight team that is capable of accomplishing missions anywhere in the United States while contributing to a totally integrated NAS comprised of all aircraft systems that can be used jointly for natural resources management. At the same time, it is hoped that FAA regulations will change in the future based on the capabilities and experience of the UAS flight team and on the locale in which operations take place, especially over large, remote, and sparsely populated areas. C1 [Rango, Albert] USDA ARS, Las Cruces, NM 88003 USA. [Laliberte, Andrea S.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Rango, A (reprint author), USDA ARS, 2995 Knox St, Las Cruces, NM 88003 USA. EM alrango@nmsu.edu; alaliber@nmsu.edu FU USDA Agricultural Research Service; National Science Foundation FX This research was funded by the USDA Agricultural Research Service and the National Science Foundation Long-Term Ecological Research Program, Jornada Basin LTER V: Landscape Linkages in Arid and Semiarid Systems. Much of our accomplishments in UAS applications are a result of the hard work, dedication, and cooperative nature of the Jornada Experimental Range UAS flight crew. We also thank the personnel of the UAS Flight Test Center, New Mexico State University - Physical Science Laboratory for their close tutoring in how to access the NAS. The authors would also like to acknowledge early reviews of this paper by Kris Havstad, Bruce Tarbert, and Dennis Zaklan and the technical support of Ms. Bernice Gamboa. NR 18 TC 24 Z9 26 U1 3 U2 38 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD JUL 13 PY 2010 VL 4 AR 043539 DI 10.1117/1.3474649 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 650AN UT WOS:000281819100001 ER PT J AU Picciani, PHS Medeiros, ES Pan, ZL Wood, DF Orts, WJ Mattoso, LHC Soares, BG AF Picciani, Paulo H. S. Medeiros, Eliton S. Pan, Zhongli Wood, Delilah F. Orts, William J. Mattoso, Luiz H. C. Soares, Bluma G. TI Structural, Electrical, Mechanical, and Thermal Properties of Electrospun Poly(lactic acid)/Polyaniline Blend Fibers SO MACROMOLECULAR MATERIALS AND ENGINEERING LA English DT Article DE conducting polymers; electrospinning; fiber characterization; nanofibers; polymer blends ID POLYANILINE; NANOFIBERS; SENSOR; FABRICATION; MORPHOLOGY; NANOTUBES; MEMBRANES; SOLVENTS AB Conducting electrospun fiber mats based on PLA and PAni blends were obtained with average diameter values between 87 and 1 006 nm with PAni quantities from 0 to 5.6 wt.-%. Structural characteristics of fiber mats were compared to cast films with the same amount of PAni and studied by SEM, SAXS, and AFM. Thermal properties of fiber mats and cast films were compared by DSC analyses. Mechanical properties of fiber mats were also evaluated. It was found that electrospinning process governs the crystal structure of the fibers and strongly affects fiber properties. New properties of PLA/PAni blends are reported due to the size fiber reduction. C1 [Picciani, Paulo H. S.; Soares, Bluma G.] Univ Fed Rio de Janeiro, Inst Macromol, Ctr Tecnol, BR-21945970 Rio De Janeiro, Brazil. [Medeiros, Eliton S.] Univ Fed Paraiba, Dept Mat Engn, Ctr Tecnol, BR-58051900 Joao Pessoa, Paraiba, Brazil. [Pan, Zhongli] ARS, Processed Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Mattoso, Luiz H. C.] Embrapa Instrumentacao Agropecuaria, Lab Nacl nanotecnol Aplicada Agronegocio, BR-13560970 Sao Carlos, SP, Brazil. RP Picciani, PHS (reprint author), Univ Fed Rio de Janeiro, Inst Macromol, Ctr Tecnol, POB 68525, BR-21945970 Rio De Janeiro, Brazil. EM picciani@ima.ufrj.br RI Medeiros, Eliton/C-5768-2013; Picciani, Paulo/H-8847-2013; Mattoso, Luiz H C/D-2794-2016 OI Mattoso, Luiz H C/0000-0001-7586-1014 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [210237/2006-6]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ); Brazilian Synchrotron Light Source Laboratory [D11A - SAXS1-7693, AFM-8970]; Embrapa/USDA FX The authors like to acknowledge financial support from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, project no. 210237/2006-6), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazilian Synchrotron Light Source Laboratory (Project number: D11A - SAXS1-7693 and AFM-8970), and Embrapa/USDA Labex program. The authors are in debt with Tina Williams for SEM analyses. NR 40 TC 22 Z9 24 U1 5 U2 29 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1438-7492 J9 MACROMOL MATER ENG JI Macromol. Mater. Eng. PD JUL 12 PY 2010 VL 295 IS 7 BP 618 EP 627 DI 10.1002/mame.201000019 PG 10 WC Materials Science, Multidisciplinary; Polymer Science SC Materials Science; Polymer Science GA 630VF UT WOS:000280302300003 ER PT J AU Stittelaar, KJ Lacombe, V van Lavieren, R van Amerongen, G Simon, J Cozette, V Swayne, DE Poulet, H Osterhaus, ADME AF Stittelaar, Koert J. Lacombe, Valerie van Lavieren, Rob van Amerongen, Geert Simon, James Cozette, Valerie Swayne, David E. Poulet, Herve Osterhaus, Albert D. M. E. TI Cross-clade immunity in cats vaccinated with a canarypox-vectored avian influenza vaccine SO VACCINE LA English DT Article DE Influenza; H5N1; Cat; Vaccine; Clade ID LOWER RESPIRATORY-TRACT; VIRUS H5N1; CHALLENGE INFECTION; DOMESTIC CATS; ANIMAL-MODELS; WILD BIRDS; PROTECTION; EFFICACY; RESPONSES; OUTBREAK AB Several felid species have been shown to be susceptible to infection with highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype. Infection of felids by H5N1 HPAI virus is often fatal, and cat-to-cat transmission has been documented. Domestic cats may then be involved in the transmission of infection to other animals but also to humans. A particular concern is the hypothetical role of the cat in the adaptation of the virus to mammalian species, thus increasing the pandemic risk. Therefore, the development of a HPAI vaccine for domestic cats should be considered a veterinary and also a public health priority. Here we show that vaccination of cats with a recombinant canarypox (ALVAC (R)(1)) virus, expressing the hemagglutinin (HA) of influenza virus A/chicken/Indonesia/03 (H5N1) confers protection against challenge infection with two antigenically distinct H5N1 virus isolates from humans. Despite low hemagglutination inhibiting (HI) antibody titers at the time of challenge, all vaccinated cats were protected against mortality and had reduced histopathological changes in the lungs. Importantly, viral shedding was reduced in vaccinated cats as compared to controls, suggesting that vaccination of cats could reduce the risk of viral transmission. In conclusion this study showed that the recombinant canarypox virus protected cats against homologous and heterologous H5N1 HPAI virus challenges. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Stittelaar, Koert J.; van Lavieren, Rob; van Amerongen, Geert; Simon, James; Osterhaus, Albert D. M. E.] ViroClin Biosci BV, NL-3015 GE Rotterdam, Netherlands. [Lacombe, Valerie] Merial SAS, F-01150 St Vulbas, France. [Cozette, Valerie; Poulet, Herve] Merial SAS, F-69348 Lyon 07, France. [Swayne, David E.] ARS, USDA, SE Poultry Res Lab, Athens, GA 30605 USA. [Osterhaus, Albert D. M. E.] Erasmus MC, Dept Virol, NL-3015 GE Rotterdam, Netherlands. RP Osterhaus, ADME (reprint author), Erasmus MC, Dept Virol, POB 1738, NL-3000 DR Rotterdam, Netherlands. EM herve.poulet@merial.com; a.osterhaus@erasmusmc.nl OI Osterhaus, Albert/0000-0002-6074-1172 NR 34 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD JUL 12 PY 2010 VL 28 IS 31 BP 4970 EP 4976 DI 10.1016/j.vaccine.2010.05.028 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 635MK UT WOS:000280659600017 PM 20566392 ER PT J AU Doehlert, DC McMullen, MS Riveland, NR AF Doehlert, D. C. McMullen, M. S. Riveland, N. R. TI Groat proportion in oats as measured by different methods: Analysis of oats resistant to dehulling and sources of error in mechanical dehulling SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Oat milling; groat proportion; oat dehulling AB Doehlert, D. C., McMullen, M. S. and Rive land, N. R. 2010. Groat proportion in oats as measured by different methods: Analysis of oats resistant to dehulling and sources of error in mechanical dehulling. Can. J. Plant Sci. 90: 391-397. Groat proportion is the groat yield from an oat dehulling process. We compared hand, impact and compressed-air dehulling to measure groat proportion, and evaluated sources of error. Hand dehulling was the simplest and most accurate method, because all groats and hulls can be accounted for. Mechanical methods dehulled most, but not all, oat kernels. Failure to account for oats resistant to dehulling in calculations resulted in gross errors. Oats resistant to impact dehulling did not differ in groat proportion from the general population, but differed in many physical properties. Hull structure may account the most for their resistance to dehulling. Mechanically dehulled oats consistently yielded lower groat proportions than those from hand dehulling. Since the difference cannot be attributed to oats resistant to dehulling, groats must be lost during the aspiration process, common to all mechanical methods. Uniform aspiration protocols should provide a uniform error. All groat proportion values obtained here were highly correlated among themselves, except when values were not corrected for oats resistant to dehulling. A theoretical groat proportion calculated from the ratio of the mean groat mass (collected by any means available) and the mean kernel mass yielded a groat proportion value that did not differ significantly from the hand dehulling value. C1 [Doehlert, D. C.] N Dakota State Univ, USDA, ARS, Hard Red Spring & Durum Wheat Qual Lab,Dept 7640, Fargo, ND 58108 USA. [McMullen, M. S.] N Dakota State Univ, Dept Plant Sci, Dept 7670, Fargo, ND 58108 USA. [Riveland, N. R.] NDSU Williston Res Extens Ctr, Williston, ND USA. RP Doehlert, DC (reprint author), N Dakota State Univ, USDA, ARS, Hard Red Spring & Durum Wheat Qual Lab,Dept 7640, Harris Hall,POB 6050, Fargo, ND 58108 USA. EM douglas.doehlert@ndsu.edu NR 14 TC 1 Z9 1 U1 1 U2 7 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD JUL 10 PY 2010 VL 90 IS 4 BP 391 EP 397 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 640CQ UT WOS:000281025800002 ER PT J AU O'Quin, JB Bourassa, L Zhang, DY Shockey, JM Gidda, SK Fosnot, S Chapman, KD Mullen, RT Dyer, JM AF O'Quin, Jami B. Bourassa, Linda Zhang, Daiyuan Shockey, Jay M. Gidda, Satinder K. Fosnot, Spencer Chapman, Kent D. Mullen, Robert T. Dyer, John M. TI Temperature-sensitive Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases Is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID STEAROYL-COA DESATURASE; FATTY-ACID DESATURASES; MEMBRANE-LIPID-COMPOSITION; GREEN FLUORESCENT PROTEIN; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MAMMALIAN-CELLS; YEAST-CELLS; ER AB Changes in ambient temperature represent a major physiological challenge to membranes of poikilothermic organisms. In plants, the endoplasmic reticulum (ER)-localized omega-3 fatty-acid desaturases (Fad3) increase the production of polyunsaturated fatty acids at cooler temperatures, but the FAD3 genes themselves are typically not up-regulated during this adaptive response. Here, we expressed two closely related plant FAD3 genes in yeast cells and found that their enzymes produced significantly different amounts of omega-3 fatty acids and that these differences correlated to differences in rates of protein turnover. Domain-swapping and mutagenesis experiments revealed that each protein contained a degradation signal in its N terminus and that the charge density of a PEST-like sequence within this region was largely responsible for the differences in rates of protein turnover. The half-life of each Fad3 protein was increased at cooler temperatures, and protein degradation required specific components of the ER-associated degradation pathway including the Cdc48 adaptor proteins Doa1, Shp1, and Ufd2. Expression of the Fad3 proteins in tobacco cells incubated with the proteasomal inhibitor MG132 further confirmed that they were degraded via the proteasomal pathway in plants. Collectively, these findings indicate that Fad3 protein abundance is regulated by a combination of cis-acting degradation signals and the ubiquitin-proteasome pathway and that modulation of Fad3 protein amounts in response to temperature may represent one mechanism of homeoviscous adaptation in plants. C1 [O'Quin, Jami B.; Bourassa, Linda] Univ New Orleans, Dept Biol Sci, New Orleans, LA 70124 USA. [Zhang, Daiyuan; Chapman, Kent D.] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA. [Zhang, Daiyuan; Fosnot, Spencer; Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Shockey, Jay M.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Gidda, Satinder K.; Mullen, Robert T.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON NWG 2W1, Canada. RP Dyer, JM (reprint author), 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM john.dyer@ars.usda.gov OI Shockey, Jay/0000-0002-5057-5457 FU United States Department of Agriculture (USDA); Current Research Information System (CRIS) [5347-21000-009-00D, 6435-41000-087-00D]; Natural Sciences and Engineering Research Council of Canada [217291]; Louisiana Board of Regents Governor's Biotechnology Initiative; University of Guelph Research Chair; USDA; University of North Texas; USDA through South Mountain Community College, Phoenix, AZ [2008-38422-19188] FX This work was supported by the United States Department of Agriculture (USDA), Current Research Information System (CRIS) Project 5347-21000-009-00D (to J.M.D.), CRIS Project 6435-41000-087-00D (to J.M.S.), Grant 217291 from the Natural Sciences and Engineering Research Council of Canada (to R. T. M.), Specific Cooperative Agreement 5347-21410-004-14S between the USDA and the University of North Texas, a grant from the Louisiana Board of Regents Governor's Biotechnology Initiative (to J.B. O'Q., L. B., and J.M.D.), and a University of Guelph Research Chair (to R. T. M.).; Supported by Cooperative State Research, Education, and Extension Service (CSREES) Grant 2008-38422-19188 from the USDA through the Undergraduate Bioscience Engagement Track (eUBET) program sponsored by South Mountain Community College, Phoenix, AZ. NR 81 TC 29 Z9 30 U1 1 U2 19 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD JUL 9 PY 2010 VL 285 IS 28 BP 21781 EP 21796 DI 10.1074/jbc.M110.135236 PG 16 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 620RD UT WOS:000279516100063 PM 20452984 ER PT J AU Grieshop, MJ Flinn, PW Nechols, JR AF Grieshop, Matthew J. Flinn, Paul W. Nechols, James R. TI Effects of intra- and interpatch host density on egg parasitism by three species of Trichogramma SO JOURNAL OF INSECT SCIENCE LA English DT Article DE biological control; foraging behavior; Plodia interpunctella; spatial scale; stored products; Trichogramma deion; Trichogramma ostriniae; Trichogramma pretiosum ID EUROPEAN CORN-BORER; PLANT STRUCTURAL COMPLEXITY; INDIANMEAL MOTH LEPIDOPTERA; OSTRINIA-NUBILALIS; SWEET CORN; INOCULATIVE RELEASES; BIOLOGICAL-CONTROL; FORAGING SUCCESS; ALMOND MOTH; HYMENOPTERA AB Host-foraging responses to different intra-and interpatch densities were used to assess three Trichogramma spp. (Hymenoptera: Trichogrammatidae) Trichogramma deion Pinto and Oatman, T. ostriniae Pang and Chen, and T. pretiosum Riley - as potential biological control agents for the Indian meal moth, Plodia interpunctella Hubner (Lepidoptera: Pyralidae). Single naive females were allowed 6 h to forage in Plexiglas arenas with four different spatial arrangements of host eggs, nine single-egg patches), nine four-egg patches, 36 single-egg patches, and 36 four-egg patches. No significant differences were found among species in the number of patches parasitized. As expected, all three species parasitized the most eggs in the 36 four-egg patch treatment and the least in the nine single-egg patch treatment. T. deion parasitized significantly more eggs than T. pretiosum on the nine four-egg patches. T. ostriniae parasitized significantly more patches when intrapatch density was greater, regardless of interpatch density. In contrast, T. deion only parasitized more patches at the greater intrapatch density when the interpatch density was low. Patch density had no effect on T. pretiosum. The spatial pattern of parasitism was more aggregated for T. deion and T. ostriniae in the 36 four-egg patches treatment compared to the 36 single-egg patches treatment. Therefore, intrapatch density was more important than interpatch density for T. ostriniae, and potentially for T. deion, but not for T. pretiosum. T. deion may be the best candidate for augmentative biological control because it parasitized either slightly or significantly more eggs than the other two species in all four treatments. Furthermore, the pattern of parasitism by T. deion in the 36 four-egg patches treatment was the most aggregated among the three species, suggesting a more thorough searching pattern. In contrast, T. pretiosum had the least aggregated pattern of parasitism and therefore may have used a more random foraging pattern. C1 [Grieshop, Matthew J.; Nechols, James R.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Flinn, Paul W.] USDA ARS, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. RP Grieshop, MJ (reprint author), Michigan State Univ, Dept Entomol, Ctr Integrated Plant Syst 205, E Lansing, MI 48824 USA. EM grieshop@msu.edu; paul.plinn@ars.usda.gov; jnechols@ksu.edu RI Flinn, Paul/B-9104-2013 FU USDA/CSREES [00-51101-9674] FX We thank Beverly Wilson (Kansas State University) and Kenlee Friesen (USDA-ARS) for their help with colony maintenance and experimental set up. We thank Kimberly With (KSU Division of Biology), Matthias Scholler (Biologische Beratung Berlin), Jim Campbell (USDA-ARS), and Lukasz Stelinski (University of Florida) for help with initial manuscript revision, as well as James Higgins (Kansas State University, Department of Statistics) for assistance with data analysis. We thank Synthia Penn (Beneficial Insectaries), Michael Hoffman and Jeffery Gardner (Cornell University) for providing us with biological material. This research was funded by: USDA/CSREES (RAMP) under agreement number 00-51101-9674. This is Contribution Number 08-150-J from the Kansas Agricultural Experiment Station. NR 43 TC 3 Z9 3 U1 1 U2 5 PU UNIV ARIZONA PI TUCSON PA LIBRARY C327, TUCSON, AZ 85721 USA SN 1536-2442 J9 J INSECT SCI JI J Insect Sci. PD JUL 8 PY 2010 VL 10 AR 99 PG 14 WC Entomology SC Entomology GA 622OK UT WOS:000279672900005 PM 20673123 ER PT J AU Poulos, SP Hausman, DB Hausman, GJ AF Poulos, Sylvia P. Hausman, Dorothy B. Hausman, Gary J. TI The development and endocrine functions of adipose tissue SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Review DE Adipose; Adipokine; Endocrine; Paracrine; Growth factor ID TRANSFORMING-GROWTH-FACTOR; MESSENGER-RNA EXPRESSION; PLASMINOGEN-ACTIVATOR INHIBITOR-1; MONOCYTE CHEMOATTRACTANT PROTEIN-1; DIET-INDUCED OBESITY; ADIPOCYTE APOLIPOPROTEIN-E; MOUSE 3T3-L1 ADIPOCYTES; NECROSIS-FACTOR-ALPHA; 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE-1; NUTRITIONALLY INDUCED OBESITY AB White adipose tissue is a mesenchymal tissue that begins developing in the fetus. Classically known for storing the body's fuel reserves, adipose tissue is now recognized as an endocrine organ. As such, the secretions from adipose tissue are known to affect several systems such as the vascular and immune systems and play major roles in metabolism. Numerous studies have shown nutrient or hormonal manipulations can greatly influence adipose tissue development. In addition, the associations between various disease states, such as insulin resistance and cardiovascular disease, and disregulation of adipose tissue seen in epidemiological and intervention studies are great. Evaluation of known adipokines suggests these factors secreted from adipose tissue play roles in several pathologies. As the identification of more adipokines and determination of their role in biological systems, and the interactions between adipocytes and other cells types continues, there is little doubt that we will gain a greater appreciation for a tissue once thought to simply store excess energy. (C) 2009 Elsevier Ireland Ltd. All rights reserved. C1 [Poulos, Sylvia P.] Coca Cola Co, Res & Technol, Atlanta, GA 30313 USA. [Hausman, Dorothy B.] Univ Georgia, Dept Foods & Nutr, Athens, GA 30602 USA. [Hausman, Gary J.] ARS, USDA, Athens, GA 30605 USA. RP Poulos, SP (reprint author), Coca Cola Co, Res & Technol, Atlanta, GA 30313 USA. EM sypoulos@na.ko.com; dhausman@uga.edu; ghausman@saa.ars.usda.gov OI Poulos, Sylvia/0000-0002-4476-6679 NR 255 TC 92 Z9 97 U1 2 U2 33 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD JUL 8 PY 2010 VL 323 IS 1 SI SI BP 20 EP 34 DI 10.1016/j.mce.2009.12.011 PG 15 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 615JQ UT WOS:000279131400003 PM 20025936 ER PT J AU Kovach, A Wegrzyn, JL Parra, G Holt, C Bruening, GE Loopstra, CA Hartigan, J Yandell, M Langley, CH Korf, I Neale, DB AF Kovach, Allen Wegrzyn, Jill L. Parra, Genis Holt, Carson Bruening, George E. Loopstra, Carol A. Hartigan, James Yandell, Mark Langley, Charles H. Korf, Ian Neale, David B. TI The Pinus taeda genome is characterized by diverse and highly diverged repetitive sequences SO BMC GENOMICS LA English DT Article ID GYPSY-LIKE RETROTRANSPOSON; ELLIOTTII VAR ELLIOTTII; WOOD PROPERTY TRAITS; LOBLOLLY-PINE; GENUS PINUS; INTERGENE RETROTRANSPOSONS; KARYOTYPE ANALYSIS; CONIFER GENOME; IDENTIFICATION; GYMNOSPERMS AB Background: In today's age of genomic discovery, no attempt has been made to comprehensively sequence a gymnosperm genome. The largest genus in the coniferous family Pinaceae is Pinus, whose 110-120 species have extremely large genomes (c. 20-40 Gb, 2N = 24). The size and complexity of these genomes have prompted much speculation as to the feasibility of completing a conifer genome sequence. Conifer genomes are reputed to be highly repetitive, but there is little information available on the nature and identity of repetitive units in gymnosperms. The pines have extensive genetic resources, with approximately 329000 ESTs from eleven species and genetic maps in eight species, including a dense genetic map of the twelve linkage groups in Pinus taeda. Results: We present here the Sanger sequence and annotation of ten P. taeda BAC clones and Genome Analyzer II whole genome shotgun (WGS) sequences representing 7.5% of the genome. Computational annotation of ten BACs predicts three putative protein-coding genes and at least fifteen likely pseudogenes in nearly one megabase of sequence. We found three conifer-specific LTR retroelements in the BACs, and tentatively identified at least 15 others based on evidence from the distantly related angiosperms. Alignment of WGS sequences to the BACs indicates that 80% of BAC sequences have similar copies (>= 75% nucleotide identity) elsewhere in the genome, but only 23% have identical copies (99% identity). The three most common repetitive elements in the genome were identified and, when combined, represent less than 5% of the genome. Conclusions: This study indicates that the majority of repeats in the P. taeda genome are 'novel' and will therefore require additional BAC or genomic sequencing for accurate characterization. The pine genome contains a very large number of diverged and probably defunct repetitive elements. This study also provides new evidence that sequencing a pine genome using a WGS approach is a feasible goal. C1 [Wegrzyn, Jill L.; Neale, David B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Kovach, Allen; Langley, Charles H.] Univ Calif Davis, Sect Evolut & Ecol, Davis, CA 95616 USA. [Parra, Genis; Korf, Ian] Univ Calif Davis, Genome Ctr, Div Biol Sci, Davis, CA 95616 USA. [Holt, Carson; Yandell, Mark] Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 84112 USA. [Bruening, George E.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Loopstra, Carol A.] Texas A&M Univ, Dept Ecol Sci & Management, College Stn, TX 77843 USA. [Hartigan, James] Beckman Coulter Genom, Danvers, MA 01923 USA. [Neale, David B.] US Forest Serv, Inst Forest Genet, USDA, Davis, CA 95616 USA. RP Neale, DB (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM dbneale@ucdavis.edu OI Wegrzyn, Jill/0000-0001-5923-0888 FU National Science Foundation [0501763] FX We acknowledge Douglas R. Cook and Jinliang Gao for assistance with BAC library screening, Charles Nicolet for technical support in WGS sequencing and Michela Troggio for sharing the unpublished apple genome. For scripting and programming support, we acknowledge Keith Bradnam, Minyoung Choi, Ben Figueroa, and John Liechty. Andrew J. Eckert, Jeffrey Ross-Ibarra and Monika J. Lipinski provided editorial comments. Funding for this study was provided by the National Science Foundation (grant number 0501763) to C.H. Langley and D.B. Neale. NR 83 TC 64 Z9 69 U1 1 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 7 PY 2010 VL 11 AR 420 DI 10.1186/1471-2164-11-420 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 662CX UT WOS:000282785800001 PM 20609256 ER PT J AU Fanta, GF Kenar, JA Byars, JA Felker, FC Shogren, RL AF Fanta, George F. Kenar, James A. Byars, Jeffrey A. Felker, Frederick C. Shogren, Randal L. TI Properties of aqueous dispersions of amylose-sodium palmitate complexes prepared by steam jet cooking SO CARBOHYDRATE POLYMERS LA English DT Article DE Amylose; Inclusion complex; Sodium palmitate; Starch; Steam jet cooking ID CONCENTRATED STARCH GELS; FATTY-ACID COMPLEXES; SOLID-STATE NMR; DODECYL-SULFATE; WHEAT-STARCH; X-RAY; GELATINIZATION; VISCOSITY; MIXTURES; AMYLOPECTIN AB Aqueous dispersions of high-amylose corn starch were steam jet cooked and blended with aqueous solutions of sodium palmitate to form amylose inclusion complexes for investigation of their bulk properties. The theological properties of the cooled dispersions depended on the starch concentration and varied from low-viscosity liquids (at 3.75% and 5.00% solids) to gels (at 6.64% solids). Gel formation was not caused by the formation of permanent cross-links. Viscosities of the dispersions increased when they were acidified with 0.02N HCl, and at pH 3.6, about 90% of the dispersed solid precipitated from the aqueous dispersion, due to conversion of complexed sodium palmitate into insoluble palmitic acid. Addition of sodium chloride solution also increased the viscosities of jet-cooked dispersions, and caused the amylose complexes to precipitate. The gelling properties of these preparations suggest practical applications as dispersants for lipids in foods, lotions, and water-based lubricants. Published by Elsevier Ltd. C1 [Kenar, James A.; Byars, Jeffrey A.; Felker, Frederick C.] ARS, Funct Food Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Fanta, George F.; Shogren, Randal L.] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Felker, FC (reprint author), ARS, Funct Food Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Frederick.Felker@ars.usda.gov NR 29 TC 17 Z9 19 U1 0 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JUL 7 PY 2010 VL 81 IS 3 BP 645 EP 651 DI 10.1016/j.carbpol.2010.03.026 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 610GR UT WOS:000278720200021 ER PT J AU Leathers, TD Nunnally, MS Cote, GL AF Leathers, Timothy D. Nunnally, Melinda S. Cote, Gregory L. TI Optimization of process conditions for enzymatic modification of alternan using dextranase from Chaetomium erraticum SO CARBOHYDRATE POLYMERS LA English DT Article DE Alternan; Chaetomium erraticum; Dextranase; Gum arabic; Linkage analysis; Modified alternan ID MUTANTS AB Alternan is a unique branched glucan with alternating alpha-(1 -> 6) and alpha-(1 -> 3) backbone linkages. We previously described the modification of alternan to a reduced molecular weight form using dextranase from Penicillium sp. The solution viscosity properties of this modified alternan resemble those of commercial gum arabic. In this study we optimize process conditions for modification of alternan using commercial dextranase from Chaetomium erraticum. This enzyme is considered GRAS (generally regarded as safe) and thus suitable for potential food applications. Optimal conditions were 10% alternan, pH 4.5, 50 degrees C. and 125 IU dextranase/ml (assayed at 28 degrees C, pH 5.0). Using these conditions, we scaled up production of modified alternan, permitting for the first time the isolation of separate peaks of modified alternan. Methylation analysis revealed a loss of linear 1,6 linkages during modification. Optimized conditions will be useful to produce modified alternan for applications testing. Published by Elsevier Ltd. C1 [Leathers, Timothy D.; Nunnally, Melinda S.; Cote, Gregory L.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Tim.Leathers@ars.usda.gov NR 15 TC 10 Z9 12 U1 3 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JUL 7 PY 2010 VL 81 IS 3 BP 732 EP 736 DI 10.1016/j.carbpol.2010.03.030 PG 5 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 610GR UT WOS:000278720200034 ER PT J AU Zeid, M Yu, JK Goldowitz, I Denton, ME Costich, DE Jayasuriya, CT Saha, M Elshire, R Benscher, D Breseghello, F Munkvold, J Varshney, RK Belay, G Sorrells, ME AF Zeid, M. Yu, J. K. Goldowitz, I. Denton, M. E. Costich, Denise E. Jayasuriya, C. T. Saha, M. Elshire, R. Benscher, D. Breseghello, F. Munkvold, J. Varshney, R. K. Belay, G. Sorrells, M. E. TI Cross-amplification of EST-derived markers among 16 grass species SO FIELD CROPS RESEARCH LA English DT Article DE Poaceae; Expressed sequence tag (EST); EST-SSR; CISP; Comparative mapping; Homology; Sequence conservation ID SSR-MARKERS; MICROSATELLITE MARKERS; BREAD WHEAT; BRACHYPODIUM-DISTACHYON; LOLIUM-PERENNE; ORYZA-SATIVA; SEQUENCES; TRANSFERABILITY; L.; POLYMORPHISM AB The availability of a large number of expressed sequence tags (ESTs) has facilitated the development of molecular markers in members of the grass family. As these markers are derived from coding sequences, cross-species amplification and transferability is higher than for markers designed from genomic DNA sequences. In this study, 919 EST-based primers developed from seven grass species were assessed for their amplification across a diverse panel of 16 grass species including cereal, turf and forage crops. Out of the 919 primers tested, 89 successfully amplified DNA from one or more species and 340 primers generated PCR amplicons from at least half of the species in the panel. Only 5.2% of the primers tested produced clear amplicons in all 16 species. The majority of the primers (66.9%) were developed from tall fescue and rice and these two species showed amplification rate of 41.6% and 19.0% across the panel, respectively. The highest amplification rate was found for conserved-intron scanning primers (CISP) developed from pearl millet (91%) and sorghum (75%) EST sequences that aligned to rice sequences. The primers with successful amplification identified in this study showed promise in other grass species as demonstrated in differentiating a set of 13 clones of reed canary grass, a species for which very little genomic research has been done. Sequences from the amplified PCR fragments indicated the potential for the transferable CISP markers for comparative mapping purposes. These primer sets can be immediately used for within and across species mapping and will be especially useful for minor grass species with few or no available molecular markers. (C) 2010 Elsevier B.V. All rights reserved. C1 [Zeid, M.; Yu, J. K.; Goldowitz, I.; Jayasuriya, C. T.; Benscher, D.; Munkvold, J.; Sorrells, M. E.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Yu, J. K.] Syngenta Seeds Inc, Stanton, MN 55018 USA. [Saha, M.] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA. [Denton, M. E.; Costich, Denise E.; Elshire, R.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Costich, Denise E.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Breseghello, F.] Embrapa Rice & Beans, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Munkvold, J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Varshney, R. K.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. [Belay, G.] Debre Zeit Agr Res Ctr, Ethiopian Inst Agr Res, Debre Zeit, Ethiopia. RP Sorrells, ME (reprint author), Cornell Univ, Dept Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. EM mes12@cornell.edu RI Breseghello, Flavio/J-6768-2012; Varshney, Rajeev/C-5295-2014; OI Varshney, Rajeev/0000-0002-4562-9131; Elshire, Robert/0000-0003-1753-6920 FU USDA/NRI [2001-35301-10612, 2005-35301-15728, 2006-55606-16629]; DOE-USDA [DE-A102-07ER64454]; Hatch Project [149419]; McKnight Foundation [06-448] FX The present work was partially supported by grants from the USDA/NRI project numbers 2001-35301-10612, 2005-35301-15728, and 2006-55606-16629, the DOE-USDA Plant Feedstock Genomics for Bioenergy Program (DE-A102-07ER64454), the Hatch Project 149419 and the McKnight Foundation's Collaborative Crop Research Program - African Chloridoid Cereals project (Grant No. 06-448). We would like to thank the anonymous reviewers for their useful criticism and helpful suggestions. NR 35 TC 14 Z9 15 U1 3 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 J9 FIELD CROP RES JI Field Crop. Res. PD JUL 6 PY 2010 VL 118 IS 1 BP 28 EP 35 DI 10.1016/j.fcr.2010.03.014 PG 8 WC Agronomy SC Agriculture GA 614YP UT WOS:000279096000004 ER PT J AU Liu, LS Jin, T Coffin, DR Liu, CK Hicks, KB AF Liu, LinShu Jin, Tony Coffin, David R. Liu, Cheng-Kung Hicks, Kevin B. TI Poly(lactic acid) Membranes Containing Bacteriocins and EDTA for Inhibition of the Surface Growth of Gram-Negative Bacteria SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE poly(lactic acid); bacteriocin; synergetic activity; active packaging; extrusion ID COMPOSITES; NISIN AB Films containing antibacterial reagents, ethylenediamine-tetraacetic acid disodium salt (EDTA) and Nisaplin (R), were produced by coextrusion with poly(lactic acid) in the presence of a pharmaceutical grade glycerol triacetate. The incorporation of EDTA-Nisaplin (R) particles resulted in a heterogeneous biphasic structure, as revealed by scanning electronic microscopy, con focal laser microscopy, and acoustic emission tests. The inclusion of glycerol triacetate reduced the Young's modulus and tensile strength, while enhancing the flexibility and the toughness of the resulting blends. The inclusion of the plasticizer also allowed the extrusion to occur at a temperature as low as 120 degrees C to maintain the biological activity of Nisaplin (R), which in combination with EDTA, plays a synergistic effect on suppression of the growth of the Gram-negative bacteria, E. colt O157:H7. The films thus obtained show potential as packaging materials with a wide spectrum of antimicrobial activity. (C) 2010 Wiley Periodicals, Inc.(dagger) J Appl Polyni Sci 117: 486-492, 2010 C1 [Liu, LinShu; Coffin, David R.; Hicks, Kevin B.] ARS, Crop Convers Sci & Engn Res Unit, USDA, Wyndmoor, PA 19038 USA. [Jin, Tony] ARS, Food Safety Intervent Technol Res Unit, USDA, Wyndmoor, PA 19038 USA. [Liu, Cheng-Kung] ARS, Fat Oils & Anim Co Prod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Liu, LS (reprint author), ARS, Crop Convers Sci & Engn Res Unit, USDA, Wyndmoor, PA 19038 USA. EM linshu.liu@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 NR 10 TC 18 Z9 18 U1 1 U2 11 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0021-8995 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD JUL 5 PY 2010 VL 117 IS 1 BP 486 EP 492 DI 10.1002/app.31550 PG 7 WC Polymer Science SC Polymer Science GA 594GF UT WOS:000277523500060 ER PT J AU Tatineni, S Gowda, S Dawson, WO AF Tatineni, Satyanarayana Gowda, Siddarame Dawson, William O. TI Heterologous minor coat proteins of Citrus tristeza virus strains affect encapsidation, but the coexpression of HSP70h and p61 restores encapsidation to wild-type levels SO VIROLOGY LA English DT Article DE Closterovirus; Virion assembly; Nucleocapsid; Specificity ID BROMOVIRUS CAPSID PROTEIN; MOSAIC-VIRUS; SUBGENOMIC RNAS; CLOSTEROVIRUS; SEQUENCE; RECOMBINATION; INFECTION; MOVEMENT; GENOME; VIRION AB The long flexuous bipolar virions of Citrus tristeza virus (CTV), a Closterovirus, are encapsidated with two capsid proteins at opposite ends: the minor coat protein (CPm) encapsidates the 5' 630 nts of the genomic RNA and the major coat protein encapsidates the remainder of the genome. In this study, we found encapsidation of DV CPm in the absence of other assembly-related proteins is highly specific in contrast to most plant viruses that allow virion assembly by a range of heterologous coat proteins. Heterologous CPms with 95-96% amino acid identity from related strains in CTV-CPm, a replicon with CPm as the only assembly-related ORF, either failed to initiate encapsidation or reduced encapsidation substantially. Substitution of subsets of amino acids revealed that the amino acids that differ between positions 121 and 180 of the VT strain, and 61 and 120 of the T3 strain were involved in specific encapsidation. We further mapped the specific encapsidation to a single amino acid: mutation of methionine(165) to threonine (VT type) or serine(105) to proline (T3 type) in CTV-CPm failed to form nucleocapsids. However, the heterologous CPm in combination with both HSP70h and p61 proteins, but not HSP70h or p61 alone, encapsidated at wild-type levels, suggesting that specific encapsidation by CPm was mitigated by the combination of HSP70h and p61. Thus, in addition to the previously described functions of HSP70h and p61 of greatly enhanced virion formation and restriction of CPm encapsidation to the 5' 630 nts of the genomic RNA. these proteins facilitate encapsidation by heterologous CPms. Published by Elsevier Inc. C1 [Tatineni, Satyanarayana; Gowda, Siddarame; Dawson, William O.] Univ Florida, IFAS, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. [Tatineni, Satyanarayana] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Tatineni, Satyanarayana] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Dawson, WO (reprint author), Univ Florida, IFAS, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA. EM wodtmv@crec.ifas.ufl.edu FU Florida Citrus Production Research Advisory Board; National Research Initiative for the USDA Cooperative State Research, Education and Extension Service FX We thank John Cook for excellent technical assistance. This research was supported in part by an endowment from the J. R. and Addie S. Graves family and grants from the Florida Citrus Production Research Advisory Board, and the National Research Initiative for the USDA Cooperative State Research, Education and Extension Service. NR 34 TC 9 Z9 9 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUL 5 PY 2010 VL 402 IS 2 BP 262 EP 270 DI 10.1016/j.virol.2010.03.042 PG 9 WC Virology SC Virology GA 603JC UT WOS:000278203800006 PM 20399478 ER PT J AU Williams, DJ Matile-Ferrero, D Miller, DR AF Williams, Douglas J. Matile-Ferrero, Daniele Miller, Douglass R. TI A study of some species of the genus Stictococcus Cockerell (Hemiptera: Sternorrhyncha: Coccoidea: Stictococcidae), and a discussion on Stictococcus vayssierei Richard, a species injurious to cassava in Equatorial Africa with a description of a new species from Nigeria SO ZOOTAXA LA English DT Article DE Scale insects; Stictococcus; Manihot esculenta; damage to roots; new species; description of adult females; key to genera; key to species ID BIOLOGICAL-CONTROL; HOM-COCCOIDEA; SCALE; HOMOPTERA; MEALYBUG; CAMEROON AB The adult female of the scale insect Stictococcus vayssierei Richard is described and illustrated. The species feeds on the root system of cassava (Manihot esculenta) (Euphorbiaceae) in Equatorial Africa, affecting tuber formation of the plant. Although damage has been reported only recently, the species has probably remained unnoticed because of its subterranean habit. The distribution and host plants of this species are listed and the segmentation of the adult female of Stictococcus is discussed to help describe the characters in detail when specimens are prepared on microscope slides. Six other species of Stictococcus are described or discussed: S. formicarius Newstead, S. intermedius Newstead, S. pujoli Richard, S. sjostedti Cockerell & Cockerell, S. subterreus Williams, Matile-Ferrero & Miller sp. n., and S. formicarius var. tuberculata Laing which is here raised to specific rank as S. tuberculatus Laing. C1 [Miller, Douglass R.] ARS, USDA, Systemat Entomol Lab, PSI, Beltsville, MD 20705 USA. [Williams, Douglas J.] Nat Hist Museum, Dept Entomol, London SW7 5BD, England. [Matile-Ferrero, Daniele] Museum Natl Hist Nat, UMR MNHN CNRS Origine Struct & Evolut Biodivers 7, Dept Systemat & Evolut, F-75231 Paris 05, France. RP Miller, DR (reprint author), ARS, USDA, Systemat Entomol Lab, PSI, Bldg 005,Barc W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM douglass.miller@ars.usda.gov NR 33 TC 1 Z9 2 U1 0 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUL 5 PY 2010 IS 2527 BP 1 EP 27 PG 27 WC Zoology SC Zoology GA 619MA UT WOS:000279433100001 ER PT J AU Stevens, ED Dowd, MK Johnson, GP French, AD AF Stevens, Edwin D. Dowd, Michael K. Johnson, Glenn P. French, Alfred D. TI Experimental and theoretical electron density distribution of alpha,alpha-trehalose dihydrate SO CARBOHYDRATE RESEARCH LA English DT Article; Proceedings Paper CT 15th European Carbohydrate Symposium CY JUL 19-24, 2009 CL Vienna, AUSTRIA DE Carbohydrate; Crystal structure; Disaccharide; Energy; Gradient vector field; Mycose ID CRYSTAL-STRUCTURE; CHARGE-DENSITY; HYDROGEN-BONDS; CONFORMATIONAL-ANALYSIS; DIFFRACTION DATA; TREHALOSE; STABILITY; SUCROSE; GLUCOSE; ENERGY AB alpha,alpha-Trehalose is of interest because of its cryoprotective and antidessicant properties, and because it possesses various technical anomalies such as (13)C NMR spectra that give misleading indications of intramolecular structural symmetry. It is a non-reducing disaccharide, with the glycosidic oxygen atom shared by the anomeric carbon atoms of the two glucose rings, and is therefore subject to a proposed 'overlapping' exo-anomeric effect. We report here a study of the electron density of trehalose with X-ray diffraction and quantum mechanics calculations, similar to a recent study of sucrose, also a non-reducing molecule. In particular we studied the electron density around the glycosidic linkage and the hydrogen bonding with both deformation density and Atoms in Molecules (AIM) analyses. A total of 129,952 single crystal X-ray intensity measurements were collected on alpha,alpha-trehalose dihydrate to a resolution of sin theta/lambda = 1.18 angstrom(-1) at 100 K and refined with an aspherical multipole model to a final agreement factor of R(1) = 0.0160. Wavefunctions were calculated at three levels of theory. Redistribution of electron density due to anomeric effects was reduced in trehalose, compared to sucrose. Five new C-H center dot center dot center dot O hydrogen bonds were confirmed with bond critical points and bond paths from AIM analyses, as were the previously proposed O-H center dot center dot center dot O hydrogen bonds. (c) 2010 Elsevier Ltd. All rights reserved. C1 [Stevens, Edwin D.] Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. [Dowd, Michael K.; Johnson, Glenn P.; French, Alfred D.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Stevens, ED (reprint author), Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. EM estevens@uno.edu; Al.French@ars.usda.gov NR 64 TC 8 Z9 8 U1 2 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0008-6215 J9 CARBOHYD RES JI Carbohydr. Res. PD JUL 2 PY 2010 VL 345 IS 10 SI SI BP 1469 EP 1481 DI 10.1016/j.carres.2010.03.017 PG 13 WC Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA 632WR UT WOS:000280460600031 PM 20381017 ER PT J AU Whelan, M Kinsella, B Furey, A Moloney, M Cantwell, H Lehotay, SJ Danaher, M AF Whelan, Michelle Kinsella, Brian Furey, Ambrose Moloney, Mary Cantwell, Helen Lehotay, Steven J. Danaher, Martin TI Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography-tandem mass spectrometry with rapid polarity switching SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article DE Anthelmintics; UHPLC-MS/MS; Polarity switching; QuEChERS; Milk ID BOVINE-MILK; BENZIMIDAZOLE RESIDUES; BIOLOGICAL MATRICES; PESTICIDE-RESIDUES; CLOSANTEL RESIDUES; IVERMECTIN; MOXIDECTIN; LIVER; EPRINOMECTIN; QUANTITATION AB A new UHPLC-MS/MS (ultra high performance liquid chromatography coupled to tandem mass spectrometry) method was developed and validated to detect 38 anthelmintic drug residues, consisting of benzimidazoles, avermectins and flukicides. A modified QuEChERS-type extraction method was developed with an added concentration step to detect most of the analytes at <1 mu g kg(-1) levels in milk. Anthelmintic residues were extracted into acetonitrile using magnesium sulphate and sodium chloride to induce liquid-liquid partitioning followed by dispersive solid phase extraction for cleanup. The extract was concentrated into dimethyl sulphoxide, which was used as a keeper to ensure analytes remain in solution. Using rapid polarity switching in electrospray ionisation, a single injection was capable of detecting both positively and negatively charged ions in a 13 min run time. The method was validated at two levels: the unapproved use level and at the maximum residue level (MRL) according to Commission Decision (CD) 2002/657/EC criteria. The decision limit (CC alpha) of the method was in the range of 0.14-1.9 and 11-123 mu g kg(-1) for drugs validated at unapproved and MRL levels, respectively. The performance of the method was successfully verified for benzimidazoles and levamisole by participating in a proficiency study. (C) 2010 Elsevier B.V. All rights reserved. C1 [Whelan, Michelle; Kinsella, Brian; Moloney, Mary; Cantwell, Helen; Danaher, Martin] TEAGASC, Food Safety Dept, Ashtown Food Res Ctr, Dublin 15, Ireland. [Whelan, Michelle; Kinsella, Brian; Furey, Ambrose] Cork Inst Technol, Team Elucidate, Dept Chem, Cork, Ireland. [Lehotay, Steven J.] ARS, USDA, Eastern Reg Ctr, Wyndmoor, PA 19038 USA. RP Danaher, M (reprint author), TEAGASC, Food Safety Dept, Ashtown Food Res Ctr, Dublin 15, Ireland. EM martin.danaher@teagasc.ie RI Danaher, Martin/M-3605-2013; OI Danaher, Martin/0000-0003-4060-0441; Furey, Ambrose/0000-0003-4119-4318 FU Irish Department of Agriculture, Fisheries and Food [06RDTMFRC434, 06RDTAAFRC479]; EU [FOOD-CT-2006-36241]; Federal Office of Consumer Protection and Food Safety (BVL) (European Community Reference Laboratory) FX This research was funded by the Irish Department of Agriculture, Fisheries and Food under the Food Institutional Research Measure as part of the National Development Plan (Projects 06RDTMFRC434 and 06RDTAAFRC479) and by EU Sixth Framework programme on Food Quality and Safety, ProSafeBeef project FOOD-CT-2006-36241. The Federal Office of Consumer Protection and Food Safety (BVL) (European Community Reference Laboratory) is acknowledged for support through organisation of proficiency test studies. James Morphet (Waters, Manchester) is acknowledged for technical support in setting up of UHPLC-MS/MS conditions. Patrick Whelan is acknowledged for supplying incurred milk samples. NR 32 TC 75 Z9 78 U1 1 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR A JI J. Chromatogr. A PD JUL 2 PY 2010 VL 1217 IS 27 BP 4612 EP 4622 DI 10.1016/j.chroma.2010.05.007 PG 11 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 615ME UT WOS:000279138600013 PM 20564781 ER PT J AU Lopez, JD Latheef, MA Hoffmann, WC AF Lopez, Juan D., Jr. Latheef, M. A. Hoffmann, W. C. TI Effect of emamectin benzoate on mortality, proboscis extension, gustation and reproduction of the corn earworm, Helicoverpa zea SO JOURNAL OF INSECT SCIENCE LA English DT Article DE emamectin benzoate; Helicoverpa zea; feeding; adult control; attracticide; larval hatch; mating; pupation ID NOCTUIDAE MOTHS; LEPIDOPTERA; EMERGENCE; EFFICACY; TRAPS AB Newly emerged corn earworm adults, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) require a carbohydrate source from plant or other exudates and nectars for dispersal and reproduction. Adults actively seek and forage at feeding sites upon eclosion in the habitat of the larval host plant or during dispersal to, or colonization of, a suitable reproductive habitat. This nocturnal behavior of H. zea has potential for exploitation as a pest management strategy for suppression using an adult feeding approach. This approach entails the use of a feeding attractant and stimulant in combination with a toxicant that when ingested by the adult will either reduce fecundity/fertility at sub-lethal dosages or kill the adult. The intent of this study was to assess reproductive inhibition and toxicity of emamectin benzoate on H. zea when ingested by the adults when mixed in ppm active ingredient (wt:vol) with 2.5 M sucrose as a feeding stimulant. Because the mixture has to be ingested to function, the effect of emamectin benzoate was also evaluated at sub-lethal and lethal concentrations on proboscis extension and gustatory response of H. zea in the laboratory. Feral males captured in sex pheromone-baited traps in the field were used for toxicity evaluations because they were readily available and were more representative of the field populations than laboratory-reared adults. Laboratory-reared female moths were used for reproduction effects because it is very difficult to collect newly emerged feral females from the field. Emamectin benzoate was highly toxic to feral H. zea males with LC(50) values (95% CL) being 0.718 (0.532-0.878), 0.525 (0.316-0.751), and 0.182 (0.06-0.294) ppm for 24, 48 and 72 h responses, respectively. Sub-lethal concentrations of emamectin benzoate did not significantly reduce proboscis extension response of feral males and gustatory response of female H. zea. Sublethal concentrations of emamectin benzoate significantly reduced percent larval hatch of eggs and mating frequency of female H. zea. Larval survival to the pupal stage was also significantly reduced by ingestion of emamectin benzoate by female H. zea. These data suggest that emamectin benzoate is a useful toxicant in an attract-and-kill control strategy against H. zea. Field studies are warranted to validate the results reported in this study. C1 [Lopez, Juan D., Jr.; Latheef, M. A.; Hoffmann, W. C.] ARS, USDA, SPA, SPARC,Areawide Pest Management Res Unit, College Stn, TX USA. RP Lopez, JD (reprint author), ARS, USDA, SPA, SPARC,Areawide Pest Management Res Unit, College Stn, TX USA. EM juan.lopez@ars.usda.gov; mohamed.latheef@ars.usda.gov; clint.hoffmann@ars.usda.gov NR 29 TC 4 Z9 6 U1 0 U2 6 PU UNIV ARIZONA PI TUCSON PA LIBRARY C327, TUCSON, AZ 85721 USA SN 1536-2442 J9 J INSECT SCI JI J Insect Sci. PD JUL 2 PY 2010 VL 10 AR 89 PG 16 WC Entomology SC Entomology GA 622OF UT WOS:000279672400003 PM 20673074 ER PT J AU Chen, KG Tian, SL Yandell, BS Kaeppler, SM An, YQC AF Chen, Kegui Tian, Shulan Yandell, Brian S. Kaeppler, Shawn M. An, Yong-qiang Charles TI Loss-of-function of DELLA protein SLN1 activates GA signaling in barley aleurone SO ACTA PHYSIOLOGIAE PLANTARUM LA English DT Article DE Aleurone; Gibberellic acid; DELLA; SLN1; Hordeum vulgare; Transcripts ID E3 UBIQUITIN LIGASE; GAMYB-LIKE GENES; SEED-GERMINATION; ALPHA-AMYLASE; GIBBERELLIN RESPONSES; ARABIDOPSIS-THALIANA; FLOWER DEVELOPMENT; FLORAL DEVELOPMENT; ANTHER DEVELOPMENT; ABSCISIC-ACID AB Gibberellic acid (GA) is an important signaling molecule that participates in many aspects of plant growth and development. While the importance of this hormone is clear, the transcriptional regulatory networks involved are still being characterized. The cereal aleurone, particularly the barley aleurone, has been used as a classic model to study GA and GA signaling for many years, and these studies have significantly contributed to our understanding of GA in plant biology. The objective of this study was to characterize the transcripts regulated through the DELLA protein SLN1, a negative regulator of the GA signaling pathway. To detect the transcripts, Affymetrix Barley 1 GeneChips were hybridized with RNA extracted from barley aleurone treated with GA or aleurone of the DELLA mutant sln1c without GA treatment. The transcripts detected, in term of both expressed genes and their function, were highly similar between the GA-treatment and the sln1c mutant. These results from a genome-wide transcript analysis provide evidence that SLN1 in the GA signal transduction pathway controls almost all GA-induced genes in the barley aleurone. C1 [Chen, Kegui; Kaeppler, Shawn M.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Tian, Shulan] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Yandell, Brian S.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Yandell, Brian S.] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA. [An, Yong-qiang Charles] ARS, USDA, Plant Genet Res Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. RP Chen, KG (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM kchen@desu.edu; yong-qiang.an@ars.usda.gov OI Kaeppler, Shawn/0000-0002-5964-1668 FU USDA ARS; Cereal Crops Research Unit CRIS; North American Barley Genome Mapping Project FX The authors thank Stacey Madson, and Sandra BonDurant for their technical help, Dr. Lishuang Shen (Virtual Reality Applications Center, Iowa State University, Ames, IA, USA) for searching BarleyBase, Dr. Peter M. Chandler (Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australia) for kindly providing sln1 mutants and treatment method, and Dr. Ron Skadsen and Dr. Li Lin for their critical reading and thoughtful comments in the manuscript. This research was partially funded by USDA ARS, Cereal Crops Research Unit CRIS fund and North American Barley Genome Mapping Project. NR 70 TC 4 Z9 6 U1 1 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0137-5881 J9 ACTA PHYSIOL PLANT JI Acta Physiol. Plant. PD JUL PY 2010 VL 32 IS 4 BP 789 EP 800 DI 10.1007/s11738-010-0464-3 PG 12 WC Plant Sciences SC Plant Sciences GA 611IW UT WOS:000278809800022 ER PT J AU Sigua, GC AF Sigua, Gilbert C. TI Sustainable cow-calf operations and water quality: A review SO AGRONOMY FOR SUSTAINABLE DEVELOPMENT LA English DT Review DE bahiagrass; cow-calf; groundwater; surface water; groundwater; sustainability; nutrient cycling; plant uptake; water quality; subtropics; BMPs; eutrophication; trophic state index (TSI) ID BEEF-CATTLE OPERATIONS; TROPHIC STATE INDEX; INDIAN RIVER LAGOON; SOIL-PHOSPHORUS; CROPPING SYSTEMS; NORTHEAST USA; NITROGEN; DYNAMICS; NUTRIENT; FLORIDA AB As animal agriculture has evolved to larger production operations in subtropical regions of United States, the problems associated with manure handling, storage and disposal have grown significantly. Understanding the interaction effects of sustainable cow farming with water-table management, nutrient dynamics and water quality in pastures could be the key to reducing nutrients in runoff. Soils do not contribute equally to nutrient export from watersheds or have the same potential to transport nutrient to runoff nor would soil test levels accurately predict total dissolved nutrients. Better understanding of soil nutrient dynamics and crop nutrient changes resulting from different management systems should allow us to predict potential impact on adjacent surface waters. In many states, these issues are critical and of increasing importance among environmentalists, ranchers, and public officials particularly in the case of N and P. One of the first steps in assessing N or P level on any farm is to consider total N or total P inputs and outputs. In Florida, reduction of P transport to receiving water bodies is the primary focus of several studies because P has been found to be the limiting nutrient for eutrophication in many aquatic systems. Long-term monitoring of the changes in soil nutrients, especially soil P would enable us to predict soil chemical or physical deterioration under continuous forage-livestock cultivation and to adopt measures to correct them before they actually happen. Despite substantial measurements using both laboratory and field techniques, little is known about the spatial and temporal variability of nutrient dynamics across the entire landscape, especially in agricultural landscapes with cow-calf operations. C1 ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. RP Sigua, GC (reprint author), ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. EM gilbert.sigua@ars.usda.gov NR 101 TC 6 Z9 6 U1 2 U2 26 PU EDP SCIENCES S A PI LES ULIS CEDEX A PA 17, AVE DU HOGGAR, PA COURTABOEUF, BP 112, F-91944 LES ULIS CEDEX A, FRANCE SN 1774-0746 J9 AGRON SUSTAIN DEV JI Agron. Sustain. Dev. PD JUL-SEP PY 2010 VL 30 IS 3 BP 631 EP 648 DI 10.1051/agro/2010001 PG 18 WC Agronomy; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY SC Agriculture; Science & Technology - Other Topics GA 647JY UT WOS:000281616000008 ER PT J AU Solari, F Shanahan, JF Ferguson, RB Adamchuk, VI AF Solari, Fernando Shanahan, John F. Ferguson, Richard B. Adamchuk, Viacheslav I. TI An Active Sensor Algorithm for Corn Nitrogen Recommendations Based on a Chlorophyll Meter Algorithm SO AGRONOMY JOURNAL LA English DT Article ID USE EFFICIENCY; FERTILIZER RECOMMENDATIONS; WINTER-WHEAT; MAIZE; DEFICIENCY; REQUIREMENTS AB In previous research we found active canopy sensor reflectance assessments of corn (Zea mays L.) N status have potential for directing in-season N applications, but emphasized an algorithm was needed to translate sensor readings into appropriate N application (N(app)) rates. The objectives of this work were to: (i) develop an active canopy sensor algorithm based on a SPAD chlorophyll meter algorithm and (ii) validate the active canopy sensor algorithm using data collected from a companion study. We derived the sensor algorithm using a linear relationship between sensor sufficiency index (SI(sensor)) and SI(SPAD) values established in the previous research and a published SPAD algorithm employing a quadratic equation to calculate N(app) as a function of SI(SPAD). The resulting equation: N(app) = 317 root 0.97-SI(sensor) represents the function for translating SI(sensor) to N(app). To validate the algorithm, SI(sensor) or values collected from small plots receiving varying N amounts were converted into N(app) using the algorithm. Then N(app) was converted into crop N balance (Nbajance) estimates, where N balance - applied N- ri N(app). Negative N(balance) values indicate N deficiency while positive values indicate excess N. The N(balance) values were compared with relative yields and a quadratic-plateau model fit to the data set for both growth stages (V11 and V15), producing an R(2) of 0.66. Relative yields plateaued at an N(balance) near zero (-11 kg N ha(-1)), indicating the algorithm provided reasonable estimates of N(app) for maximizing yields. The equation provides a basis for the use of active crop canopy sensors for in-season N management of irrigated corn C1 [Shanahan, John F.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Solari, Fernando] Monsanto Co, Pergamino, Buenos Aires, Argentina. [Ferguson, Richard B.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Adamchuk, Viacheslav I.] Univ Nebraska, Dep Biol Syst Engn, Lincoln, NE 68583 USA. RP Shanahan, JF (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM John.Shanahan@ars.usda.gov OI Shanahan, John/0000-0003-3173-4245 FU USDA; NASA FX This work was supported in part by the project Thematic Soil Mapping and Crop-Based Strategies for Site-Specific Management jointly funded by USDA and NASA under the Initiative for Future Agriculture and Food Systems (IFAFS) program on Application of Geospatial and Precision Technologies (AGPT). NR 29 TC 18 Z9 19 U1 2 U2 19 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1090 EP 1098 DI 10.2134/agronj2010.0009 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300002 ER PT J AU Cassida, KA Foster, JG Turner, KE AF Cassida, K. A. Foster, J. G. Turner, K. E. TI Forage Characteristics Affecting Meat Goat Preferences for Forage Chicory Cultivars SO AGRONOMY JOURNAL LA English DT Article ID TALL FESCUE HAYS; SESQUITERPENE LACTONES; NUTRITIVE-VALUE; CICHORIUM-INTYBUS; GRAZING MANAGEMENT; RUMINANT LIVESTOCK; PARASITE CONTROL; SHEEP; PHOSPHORUS; MAGNESIUM AB Concentration of bitter sesquiterpene lactones (SL), lactucin, lactucopicrin, and 8-deoxylactucin, has been associated with low soil P fertility and reduced livestock preference for forage chicory (Cichorium intybus L.). We evaluated the effect of cultivar and available soil phosphorus (ASP) on meat goat (Capra hircus L.) preferences for 'Grasslands Puna (Puna)' 'Forage Feast,' and 'INIA le Lacerta (Lacerta)'chicory grown under varying P fertility. Soil Bray-P was used to designate ASP as low (LP, mean 8.6 mg kg(-1)), medium (MP, 21.7 mg kg(-1)), and high (HP, 40.1 mg kg(-1)) for the preference test. In September, clipped fresh forage was offered to confined Boer-cross goat kids using a replicated 3 by 3 Latin square design with an extra period. Preferences were measured using dry matter intake for cultivar effects and multidimensional scaling (MDS) for ASP effects. Across cultivars, soil Bray-P was positively related to leaf P and Mg concentrations. Soil Bray-P did not affect lactucopicrin or 8-deoxylactucin, and was positively associated with lactucin, in contrast to the hypothesis. Goats exhibited no preferences among cultivars despite up to fourfold differences in individual SL. Within cultivars, goats preferred Puna with less lactucin and total SL, Lacerta with less lactucopicrin and leaf Mg, and Forage Feast with more cell wall. Goats were able to detect relatively small differences in SL within cultivars, but this had little practical effect on intake of the forage because they ate it even when bitter. Therefore, P fertilization for fall-grown chicory is unlikely to improve forage intake in goats. C1 [Cassida, K. A.; Foster, J. G.; Turner, K. E.] ARS, Appalachian Farming Syst Res Ctr, USDA, Beaver, WV 25813 USA. RP Cassida, KA (reprint author), ARS, Appalachian Farming Syst Res Ctr, USDA, 1224 Airport Rd, Beaver, WV 25813 USA. EM kim.cassida@ars.usda.gov NR 52 TC 5 Z9 5 U1 1 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1109 EP 1117 DI 10.2134/agronj2009.0500 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300004 ER PT J AU Burke, JJ Franks, CD Burow, G Xin, Z AF Burke, J. J. Franks, C. D. Burow, G. Xin, Z. TI Selection System for the Stay-Green Drought Tolerance Trait in Sorghum Germplasm SO AGRONOMY JOURNAL LA English DT Article AB Post-flowering drought tolerance is an essential trait for increasing the production of sorghum [Sorghum bicolor (L.) Moench] and other cereals in Mediterranean and semiarid tropical climates. Current methodologies for identifying the nonsenescent (stay-green) trait require the right intensity of drought stress at the right developmental stage to visually evaluate lines in the field. Field-based evaluations of drought tolerance are notoriously difficult to manage, and often require growing lines in multiple locations across several years to acquire a meaningful assessment of the stay-green trait. By means of a 30-min high-temperature challenge to leaf tissue during flowering of well-watered sorghum and a 30-min room temperature recovery, we show that stay-green lines can be readily identified. Using chlorophyll fluorescence to monitor tissue injury, we found that tissue with higher intercellular sucrose concentrations exhibited higher chlorophyll fluorescence yield following the temperature challenge. Stay-green lines evaluated in this study maintained higher dawn leaf sucrose levels than the senescent lines among the five youngest leaf positions. Evaluation of 10 known stay-green and senescent sorghum lines, previously reported in the literature, with this bioassay allowed us to separate the two classes of sorghum from well-watered flowering plants. The stay-green lines can also be separated from senescent lines under well-watered greenhouse conditions from the boot stage onward. This technology will greatly reduce the selection time needed to identify drought tolerant sorghum. C1 [Burke, J. J.; Burow, G.; Xin, Z.] USDA, Plant Stress & Germplasm Dev Unit, Lubbock, TX 79415 USA. [Franks, C. D.] Pioneer HiBred Int Inc, Plainview, TX 79072 USA. RP Burke, JJ (reprint author), USDA, Plant Stress & Germplasm Dev Unit, 3810 4th St, Lubbock, TX 79415 USA. EM John.Burke@ars.usda.gov OI Xin, Zhanguo/0000-0003-1471-7785 NR 10 TC 7 Z9 11 U1 2 U2 9 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1118 EP 1122 DI 10.2134/agronj2009.0465 PG 5 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300005 ER PT J AU Carter, JE Jokela, WE Bosworth, SC AF Carter, Jeffrey E. Jokela, William E. Bosworth, Sidney C. TI Grass Forage Response to Broadcast or Surface-Banded Liquid Dairy Manure and Nitrogen Fertilizer SO AGRONOMY JOURNAL LA English DT Article ID AMMONIA VOLATILIZATION; CATTLE SLURRY; SOIL-NITROGEN; YIELD; CORN; EMISSIONS; ORCHARDGRASS; PHOSPHORUS; INJECTION; WATERS AB Manure can provide valuable nutrients, especially N, for grass forage, but N availability is limited because of high NH(3) volatilization losses from standard surface-broadcast application. Field experiments WC CC conducted for 2 yr at two sites in Vermont to evaluate effects of broadcast or banded liquid dairy manure and broadcast N fertilizer on grass yield and N utilization. Treatments were applied to orchardgrass (Dactylis glomerata L.) on a well-drained silt loam and to reed canarygrass (Phalaris arundinacea L.) on a somewhat poorly drained clay soil. Manure was applied three or four times per year at rates of 25 or 50 m(3) ha(-1) either by broadcasting or by trailing-foot spreading in narrow bands. Fertilizer N was broadcast at 0, 28, 56, and 84 kg N ha(-1) on separate plots at the same time as each manure application. Fertilizer N increased yields significantly to the medium rate (224 kg ha(-1) yr(-1)) on orchardgrass and the high rate (252 kg ha(-1) yr(-1)) on the reed canarygrass site. The high rate of banded manure produced 80 to 110% of the yields from the high N fertilizer rate. Yields from the trailing-foot, banded manure application were 6 to 14% higher than those from broadcast manure in the two site-years where method had a significant effect. Fertilizer N equivalence of manure averaged 44% with banded and 34% with broadcast application. We conclude that surface-banding manure with a trailing-foot applicator has the potential to provide benefits over conventional broadcast application by improving N utilization and increasing yield. C1 [Jokela, William E.] ARS, USDA, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. [Carter, Jeffrey E.] Univ Vermont Extens, Middlebury, VT 05753 USA. [Bosworth, Sidney C.] Univ Vermont, Burlington, VT 05405 USA. RP Jokela, WE (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. EM bill.jokela@ars.usda.gov FU Univ. of Vermont Agric. Exp. Station Competitive Hatch Program FX We greatly appreciate the important contributions of John Rankin for field and laboratory technical assistance and statistical analysis. Financial support was provided, in part, by USDA-Hatch funds through the Univ. of Vermont Agric. Exp. Station Competitive Hatch Program. NR 41 TC 6 Z9 6 U1 4 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1123 EP 1131 DI 10.2134/agronj2009.0382 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300006 ER PT J AU Johnson, MVV Kiniry, JR Burson, BL AF Johnson, Mari-Vaughn V. Kiniry, James R. Burson, Byron L. TI Ceptometer Deployment Method Affects Measurement of Fraction of Intercepted Photosynthetically Active Radiation SO AGRONOMY JOURNAL LA English DT Article ID LEAF-AREA INDEX; LIGHT; ACCUMULATION; REGISTRATION; BUFFELGRASS; REFLECTANCE; COMMUNITIES; ECOSYSTEMS; GRASSES; CLIMATE AB The fraction of photosynthetically active radiation a canopy intercepts (fiPAR) drives canopy level photosynthesis. There is currently no universal, repeatable fiPAR sensor deployment method. We show variability of fiPAR measurements by three sensor deployment methods, including two 1 by 1 m and one 3 by 1 m method. The deployment method biased measurements (P = 0.005) under buffelgrass (Pennisetum ciliare L) canopies. These effects were less evident in 'Alamo' switchgrass (Panicum virgatum L) and miscanthus (Miscanthus x giganteus). Canopies of these two species showed deployment method x nutrient addition interaction effects (P = 0.02), apparently driven by nutrient effects on leaf area index (LAI). We highlight potential implications of using the different deployment methods via an exercise in the application of Beer's law. As actual LAI increased, effect of deployment method on fiPAR measurements tended to diminish, suggesting in high LAI systems a universal deployment method is not as critical as it is in low LAI systems. C1 [Johnson, Mari-Vaughn V.; Kiniry, James R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Burson, Byron L.] ARS, USDA, College Stn, TX 77845 USA. [Burson, Byron L.] Texas A&M Univ, College Stn, TX 77845 USA. RP Johnson, MVV (reprint author), ARS, USDA, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM mari-vaughn.johnson@ars.usda.gov NR 37 TC 7 Z9 7 U1 1 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1132 EP 1137 DI 10.2134/agronj2009.0478 PG 6 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300007 ER PT J AU Bauer, PJ Fortnum, BA Frederick, JR AF Bauer, Philip J. Fortnum, Bruce A. Frederick, James R. TI Cotton Responses to Tillage and Rotation during the Turn of the Century Drought SO AGRONOMY JOURNAL LA English DT Article ID COVER CROP; MELOIDOGYNE-INCOGNITA; FIBER PROPERTIES; SOIL; YIELD; NEMATODES; ALDICARB; IMPACTS; WATER; RYE AB Longer rain-free periods are predicted to occur more often in the southeastern United States as a result of global climate change. This nonirrigated field study was conducted from 1997 through 2002, which coincided with the 1998-2002 drought that affected most of the United States. The objective was to determine the effect of rotation and tillage on cotton (Gossypium hirsutum L.) productivity. Treatments in the study were rotation [cotton rotated with corn (Zea mays L.), cotton planted after a rye (Secale cereale L.) winter cover crop, and continuous cotton with no cover crop] and tillage system (conventional tillage and conservation tillage). Two levels of aldicarb [2-methyl-2-(methylthio)propanal O-{(methylamino)carbonyl}oxime] (0 and 1.18 kg a.i. ha(-1)) were also included because of known soil management effects on thrips (Frankliniella sp.) and root-knot nematodes (Meloidigyne incognita). The predominant soil types were Bonneau loamy sand (loamy, siliceous, subactive, thermic Arenic Paleudult) and Norfolk loamy sand (fine-loamy, kaolinitic, thermic Typic Kandiudult). Rotation did not affect cotton yield in any year. Tillage did not affect cotton yield in 1997. Conservation tillage resulted in an average 25% yield increase in cotton lint yield over conventional tillage during the 5-yr drought. Tillage and aldicarb affected both thrips and root-knot nematodes, but lack of interaction among these factors for lint yield suggested that management of these pests was not the predominant cause for the cotton yield increase with conservation tillage. Conservation tillage for cotton production could be an important method to help mitigate the effects of climate change in the region if change occurs as predicted. C1 [Bauer, Philip J.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Fortnum, Bruce A.; Frederick, James R.] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29506 USA. RP Bauer, PJ (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM phil.bauer@ars.usda.gov NR 21 TC 6 Z9 6 U1 3 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1145 EP 1148 DI 10.2134/agronj2010.0037 PG 4 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300009 ER PT J AU Deak, A Hall, MH Sanderson, MA Rotz, A Corson, M AF Deak, Atila Hall, Marvin H. Sanderson, Matt A. Rotz, Al Corson, Michael TI Whole-Farm Evaluation of Forage Mixtures and Grazing Strategies SO AGRONOMY JOURNAL LA English DT Article ID PENNSYLVANIA DAIRY FARMS; NUTRITIVE-VALUE; ENVIRONMENTAL IMPLICATIONS; BOTANICAL COMPOSITION; PRODUCTIVITY; PASTURE; DIVERSITY; SYSTEMS; MICHIGAN; ALFALFA AB Complex forage mixtures (mixtures of more than three species) have been researched as a means to increase yield and sustain forage production in pastures of the northeastern United States. However, little research has focused on the economic impact of forage mixture complexity and grazing strategy on a whole-farm scale. We used the Integrated Farm System Model (IFSM) to examine the short- (2-yr) and long-term (25-yr) performance and economic returns of four pasture mixtures (two, three, five, and seven species of grasses and legumes) and grass monocultures grazed according to plant morphology or canopy height criteria. For both 2- and 25-yr analyses, reduced pasture production in the morphology-based grazing strategy led to a decrease in net return compared to the height-based grazing strategy. Both analyses showed that the differences in net return were mainly due to seed, fertilizer, and feed costs, pasture production, and the income from excess forage sales. Production was more dependable for the height-based grazing strategy compared with the morphology-based strategy. Complex mixtures generated greater and more consistent net returns compared with either the simple mixtures or grass monoculture. More importantly, when comparing the difference in net return obtained by a particular forage treatment in dry and wet years, the net return using complex mixtures was reduced by only 25 to 27%. On the other hand, reductions in net return ranged from 36% for a three-species mixture to 55% for grass monoculture. For dairy pastures, complex mixtures are a useful alternative to reduce production variability in dry years. C1 [Deak, Atila] Monsanto Co, Corpus Christi, TX 78414 USA. [Hall, Marvin H.] Penn State Univ, Crop & Soil Sci, University Pk, PA 16802 USA. [Sanderson, Matt A.; Rotz, Al] ARS, USDA, Pasture Syst & Watershed Management Unit, University Pk, PA 16802 USA. [Corson, Michael] INRA, F-35000 Rennes, France. [Corson, Michael] Agrocampus Rennes, UMR Soil Agro & Hydrosyst 1069, F-35000 Rennes, France. RP Deak, A (reprint author), Monsanto Co, 6729 Newman, Corpus Christi, TX 78414 USA. EM atila.deak@monsanto.com RI Corson, Michael/A-7671-2008; Lecomte, marcel/C-4653-2012; UMR SAS, INRA/L-1751-2013; UMR SAS, ASAE/M-4978-2013 OI Corson, Michael/0000-0003-4785-8971; UMR SAS, ASAE/0000-0002-4138-7374 NR 33 TC 8 Z9 8 U1 1 U2 26 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1201 EP 1209 DI 10.2134/agronj2009.0504 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300016 ER PT J AU Blanco-Canqui, H Stone, LR Schlegel, AJ Benjamin, JG Vigil, MF Stahlman, PW AF Blanco-Canqui, Humberto Stone, L. R. Schlegel, A. J. Benjamin, J. G. Vigil, M. F. Stahlman, P. W. TI Continuous Cropping Systems Reduce Near-Surface Maximum Compaction in No-Till Soils SO AGRONOMY JOURNAL LA English DT Article ID ORGANIC-CARBON; GREAT-PLAINS; PHYSICAL-PROPERTIES; BULK-DENSITY; PROCTOR TEST; CHEMICAL-PROPERTIES; BRITISH-COLUMBIA; DRYLAND SYSTEMS; FOREST SOILS; MATTER AB Because of increased concerns over compaction in no-till (NT) soils, it is important to assess how continuous cropping systems influence risks of soil compaction across a range of soils and NT management systems. We quantified differences in maximum bulk density (BD(max)) and critical water content (CWC) by the Proctor test, field bulk density (p(b)), and their relationships with soil organic carbon (SOC) concentration across three (>11 yr) cropping systems on a silty clay loam, silt loans, and loam in the central Great Plains. On the silty clay loam, BD(max) in sorghum [Sorghum bicolor (L.) Moench]-fallow (SF) and winter wheat [Triticum aestivum (L.)]-fallow (WF) was greater than in continuous wheat (WW) and continuous sorghum (SS) by 0.1 Mg m(-3) in the 0- to 5-cm soil depth. On the loans, BD(max) in WF was greater than in W-corn (Zea mays L.)-millet (Panicum liliaceum L.) (WCM) by 0.24 Mg m(-3) and perennial grass (GRASS) by 0.11 Mg m(-3). On the silt loans, soil properties were unaffected by cropping systems. Elimination of fallowing increased the CWC by 10 to 25%. The P(b) was greater in WF (1.52 Mg m(-3)) than in WW (1.16 Mg m(-3)) in the silty clay loans, while p(b) under WF and WCF was greater than under WCM and GRASS in the loam for the 0- to 5-cm depth. The BD(max) and P(b) decreased whereas CWC increased with an increase in SOC concentration in the 0- to 15-cm depth. Overall, continuous cropping systems in NT reduced near-surface maximum soil compaction primarily by increasing SOC concentration. C1 [Blanco-Canqui, Humberto; Stahlman, P. W.] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Stone, L. R.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Schlegel, A. J.] Kansas State Univ, SW Res Extens Ctr, Tribune, KS 67879 USA. [Benjamin, J. G.; Vigil, M. F.] ARS, USDA, Cent Great Plains Res Stn, No Plains Area, Akron, CO 80720 USA. RP Blanco-Canqui, H (reprint author), Kansas State Univ, Agr Res Ctr Hays, 1232 240th Ave, Hays, KS 67601 USA. EM hblanco@ksu.edu NR 26 TC 11 Z9 12 U1 2 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1217 EP 1225 DI 10.2134/agronj2010.0113 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300018 ER PT J AU Lenssen, AW Cash, SD Hatfield, PG Sainju, UM Grey, WR Blodgett, SL Goosey, HB Griffith, DA Johnson, GD AF Lenssen, A. W. Cash, S. D. Hatfield, P. G. Sainju, U. M. Grey, W. R. Blodgett, S. L. Goosey, H. B. Griffith, D. A. Johnson, G. D. TI Yield, Quality, and Water and Nitrogen Use of Durum and Annual Forages in Two-Year Rotations SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; SEMIARID CLIMATE; CROPPING SYSTEMS; USE EFFICIENCY; LOW-RAINFALL; BARLEY; WHEAT; PEA; OAT; INTENSIFICATION AB Annual spring-seeded forage crops use less water than cereal grains, including durum (Triticum turgidum L. var. durum), and may be suitable to replace summer fallow. We conducted an experiment from 2002 through 2006 comparing yield, quality, and water and N use of durum and three annual forages, barley (Hordeum vulgare L.), barley interseeded with pea [Piston sativum L. ssp. arvense (L.) Poir.], and foxtail millet [Setaria italica (L.) Beau.] in 2-yr rotations. Durum in rotation with summer fallow and alfalfa (Medicago sativa L.) were included. Averaged over 5 yr, alfalfa had higher forage yield and quality, water use, and N accumulation compared to annual forages. Annual forages had similar preplant and postharvest soil water contents, but barley and barley-pea had higher yields and water use compared to millet. Barley-pea intercrop had superior forage crude protein (CP), neutral detergent fiber (NDF) and N accumulation compared to barley and millet, but acid detergent fiber (ADP) and nitrogen recovery index (NRI) were similar among annual forages. Averaged over 4 yr, preplant soil water and residual N content were greater for durum following fallow than for durum following annual forages, resulting in reduced fertilizer N requirement and greater yield, water use, grain N accumulation and NRI following fallow. Replacing summer fallow with annual forages reduced durum grain yield by 727 kg ha(-1) but provided forage yield of 4.9 Mg ha(-1). Annualized net returns in annual forage-durum systems were $127 ha(-1), $77 and $34 ha(-1) greater than for fallow-durum and alfalfa, respectively. Replacing summer fallow with annual forages reduced durum yield but improved profitability,. C1 [Lenssen, A. W.; Sainju, U. M.] ARS, USDA, No Plains Agr Res Lab, Sidney, MT 59270 USA. [Cash, S. D.; Hatfield, P. G.; Goosey, H. B.; Johnson, G. D.] Montana State Univ, Dep Anim & Range Sci, Bozeman, MT 59717 USA. [Grey, W. R.] Montana State Univ, Dep Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Griffith, D. A.] Montana State Univ, Dep Agr Econ & Econ, Bozeman, MT 59717 USA. [Blodgett, S. L.] S Dakota State Univ, Dep Plant Sci, Brookings, SD 57006 USA. RP Lenssen, AW (reprint author), ARS, USDA, No Plains Agr Res Lab, Sidney, MT 59270 USA. EM andy.lenssen@ars.usda.gov FU USDA; CSREES FX The authors gratefully acknowledge technical support from Matt Connor, Mark Gaffri, Larry Hagenbuch, Jill Craig, Brady Paladichuk, and Nick Paritz. The Roosevelt and Sheridan County Conservation Districts kindly provided farmland on which to conduct this research. Partial financial support for this project was provided by USDA, CSREES Special Grants 'Sustainable Pest Management in Dryland Wheat' to A.W. Lenssen, G.D. Johnson, W.R. Gray, P.G. Hatfield, and S.L. Blodgett, Department of Entomology, Montana State University, Bozeman, MT. NR 31 TC 15 Z9 14 U1 1 U2 19 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1261 EP 1268 DI 10.2134/agronj2010.0078 PG 8 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300024 ER PT J AU Brink, G Hall, M Shewmaker, G Undersander, D Martin, N Walgenbach, R AF Brink, Geoffrey Hall, Marvin Shewmaker, Glen Undersander, Dan Martin, Neal Walgenbach, Richard TI Changes in Alfalfa Yield and Nutritive Value within Individual Harvest Periods SO AGRONOMY JOURNAL LA English DT Article ID NEUTRAL DETERGENT FIBER; CELL-WALL COMPOSITION; MORPHOLOGICAL DEVELOPMENT; FIRST FLOWER; DAIRY-COWS; DIGESTIBILITY; QUALITY; FORAGE; TEMPERATURE; MATURITY AB Understanding the relationship between alfalfa (Medicago sativa L.) yield and nutritive value throughout the growing season will permit optimum timing of harvest. Our objective was to determine the rate at which alfalfa yield and fiber components change during each of four harvest periods. In spring, early summer, late summer, and fall of 2004 and 2005 at Pennsylvania, Wisconsin, and Idaho, primary growth of three alfalfa cultivars was initially harvested at late vegetative stage and every 5 d thereafter for 20 d. Forage dry matter (DM) yield, neutral detergent fiber (NDF), and in vitro neutral detergent fiber digestibility (NDFD) were measured at each harvest. Rate of DM production of all cultivars was greatest both years during the spring at Pennsylvania (222.5 and 702.2 kg ha(-1) d(-1), respectively) and early summer at Wisconsin (83.4 and 278.8 kg ha(-1) d(-1), respectively), and in early and late summer at Idaho (198.4 and 194.4 kg DM ha(-1) d(-1), respectively, in 2005 only). The rate of increase in NDF was generally greatest during spring at Pennsylvania and Wisconsin, and during early summer at Idaho, while the rate of decrease in NDFD was generally greatest during early summer at all locations. The results suggest that the negative association between yield and nutritive value has greatest impact on timing of harvests made in spring and early summer in humid environments, and in early and late summer in more arid regions. C1 [Brink, Geoffrey; Martin, Neal; Walgenbach, Richard] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Hall, Marvin] Penn State Univ, Dep Crop & Soil Sci, University Pk, PA 16802 USA. [Shewmaker, Glen] Univ Idaho, Moscow, ID 83844 USA. [Undersander, Dan] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. RP Brink, G (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM Geoffry.Brink@ars.usda.gov NR 24 TC 7 Z9 7 U1 4 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2010 VL 102 IS 4 BP 1274 EP 1282 DI 10.2134/agronj2010.0080 PG 9 WC Agronomy SC Agriculture GA 627HO UT WOS:000280030300026 ER PT J AU Belisle, SE Hamer, DH Leka, LS Dallal, GE Delgado-Lista, J Fine, BC Jacques, PF Ordovas, JM Meydani, SN AF Belisle, Sarah E. Hamer, Davidson H. Leka, Lynette S. Dallal, Gerard E. Delgado-Lista, Javier Fine, Basil C. Jacques, Paul F. Ordovas, Jose M. Meydani, Simin Nikbin TI IL-2 and IL-10 gene polymorphisms are associated with respiratory tract infection and may modulate the effect of vitamin E on lower respiratory tract infections in elderly nursing home residents SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; IFN-GAMMA GENE; ALPHA-TOCOPHEROL SUPPLEMENTATION; COMMUNITY-ACQUIRED PNEUMONIA; BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; CYTOKINE PRODUCTION; PROMOTER POLYMORPHISM; ECONOMIC BURDEN; MESSENGER-RNA AB Background: Vitamin E supplementation may be a potential strategy to prevent respiratory tract infections (RIs) in the elderly. The efficacy of vitamin E supplementation may depend on individual factors including specific single nucleotide polymorphisms (SNPs) at immunoregulatory genes. Objective: We examined whether the effect of vitamin E on RIs in the elderly was dependent on genetic backgrounds as indicated by SNPs at cytokine genes. Design: We used data and DNA from a previous vitamin E intervention study (200 IU vitamin E or a placebo daily for 1 y) in elderly nursing home residents to examine vitamin E-gene interactions for incidence of RI. We determined the genotypes of common SNPs at IL-1 beta, IL-2, IL-6, IL-10, TNF-alpha, and IFN-gamma in 500 participants. We used negative binomial regression to analyze the association between genotype and incidence of infection. Results: The effect of vitamin E on lower RI depended on sex and the SNP at IL-10 -819G-->A (P = 0.03 for interaction for lower RI). Furthermore, we observed that subjects with the least prevalent genotypes at IL-2 -330A-->C (P = 0.02 for upper RI), IL-10 -819G-->A (P = 0.08 for upper RI), and IL-10 -1082C-->T (P < 0.001 for lower RI in men) had a lower incidence of RI independent of vitamin E supplementation. Conclusions: Studies that evaluate the effect of vitamin E on RIs should consider both genetic factors and sex because our results suggest that both may have a significant bearing on the efficacy of vitamin E. Furthermore, common SNPs at cytokine genes may contribute to the individual risk of RIs in the elderly. This trial was registered at clinicaltrials.gov as NCT00758914. Am J Clin Nutr 2010; 92: 106-14. C1 [Meydani, Simin Nikbin] Tufts Univ, Nutr Immunol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Meydani, Simin Nikbin] Tufts Univ, Dept Pathol, Sackler Grad Sch Biochem Sci, Boston, MA 02111 USA. [Hamer, Davidson H.] Boston Univ, Sch Publ Hlth, Ctr Int Hlth & Dev, Boston, MA USA. [Hamer, Davidson H.] Boston Univ, Dept Med, Infect Dis Sect, Boston, MA 02215 USA. [Delgado-Lista, Javier] Univ Cordoba, Inst Maimonides Invest Biomed Cordoba IMIBIC, Lipids & Atherosclerosis Res Unit,Inst Salud Carl, Reina Sofia Univ Hosp,CIBER Fisiopatol Obesidad &, Cordoba, Spain. [Fine, Basil C.] Mt Auburn Hosp, Dept Med, Cambridge, MA USA. RP Meydani, SN (reprint author), Tufts Univ, Nutr Immunol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM simin.meydani@tufts.edu OI Hamer, Davidson/0000-0002-4700-1495; Ordovas, Jose/0000-0002-7581-5680 FU National Institute on Aging/National Institutes of Health [5R01-AG013975]; USDA [58-1950-7-707]; Hoffman LaRoche Inc; DSM Nutritional Products Inc; National Institutes of Health/National Institute on Aging [PO1 AG004390] FX Supported by grant 5R01-AG013975 from the National Institute on Aging/National Institutes of Health, USDA agreement 58-1950-7-707, a grant for the preparation of study capsules from Hoffman LaRoche Inc, a DSM Nutritional Products Inc scholarship (to SEB); National Institutes of Health/National Institute on Aging grant PO1 AG004390 (to Lewis Lipsitz), which facilitated recruitment of subjects and allowed the study to be conducted at the Hebrew Rehabilitation Center, and CIBER Fisiopatologia de la Obesidad y Nutricion, an initiative of ISCIII, government of Spain. NR 63 TC 11 Z9 12 U1 0 U2 8 PU AMER SOC CLINICAL NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, SUBSCRIPTIONS, RM L-3300, BETHESDA, MD 20814-3998 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUL 1 PY 2010 VL 92 IS 1 BP 106 EP 114 DI 10.3945/ajcn.2010.29207 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 612YX UT WOS:000278943100016 PM 20484443 ER PT J AU Marini, JC Didelija, IC Castillo, L Lee, B AF Marini, Juan C. Didelija, Inka Cajo Castillo, Leticia Lee, Brendan TI Glutamine: precursor or nitrogen donor for citrulline synthesis? SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE arginine; proline; urea cycle ID RAT SMALL-INTESTINE; L-ARGININE; ORNITHINE AMINOTRANSFERASE; PYRROLINE-5-CARBOXYLATE SYNTHASE; ISOLATED ENTEROCYTES; PARENTERAL AFFECTS; NEONATAL PIGLETS; STABLE-ISOTOPE; AMINO-ACIDS; METABOLISM AB Marini JC, Didelija IC, Castillo L, Lee B. Glutamine: precursor or nitrogen donor for citrulline synthesis? Am J Physiol Endocrinol Metab 299: E69-E79, 2010. First published April 20, 2010; doi:10.1152/ajpendo.00080.2010.-Although glutamine is considered the main precursor for citrulline synthesis, the current literature does not differentiate between the contribution of glutamine carbon skeleton vs. nonspecific nitrogen (i.e., ammonia) and carbon derived from glutamine oxidation. To elucidate the role of glutamine and nonspecific nitrogen in the synthesis of citrulline, L-[2-(15)N]- and L-[5-(15)N] glutamine and (15)N-ammonium acetate were infused intragastrically in mice. The amino group of glutamine labeled the three nitrogen groups of citrulline almost equally. The amido group and ammonium acetate labeled the ureido and amino groups of citrulline, but not the delta-nitrogen. D(5)-glutamine also infused in this arm of the study, which traces the carbon skeleton of glutamine, was utilized poorly, accounting for only 0.2-0.4% of the circulating citrulline. Dietary glutamine nitrogen (both N groups) incorporation was 25-fold higher than the incorporation of its carbon skeleton into citrulline. To investigate the relative contributions of the carbon skeleton and nonspecific carbon of glutamine, arginine, and proline to citrulline synthesis, U-(13)C(n) tracers of these amino acids were infused intragastrically. Dietary arginine was the main precursor for citrulline synthesis, accounting for similar to 40% of the circulating citrulline. Proline contribution was minor (3.4%), and glutamine was negligible (0.4%). However, the glutamine tracer resulted in a higher enrichment in the ureido group, indicating incorporation of nonspecific carbon from glutamine oxidation into carbamylphosphate used for citrulline synthesis. In conclusion, dietary glutamine is a poor carbon skeleton precursor for the synthesis of citrulline, although it contributes both nonspecific nitrogen and carbon to citrulline synthesis. C1 [Lee, Brendan] Howard Hughes Med Inst, Houston, TX 77030 USA. [Lee, Brendan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Marini, Juan C.; Didelija, Inka Cajo; Castillo, Leticia] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. RP Marini, JC (reprint author), 1100 Bates St,Mail Stop BCM320, Houston, TX 77030 USA. EM marini@bcm.edu FU US Department of Agriculture [6250-51000-044]; National Institutes of Health [K01-RR-024173, RO1-DK-54450] FX The work was supported by the US Department of Agriculture (6250-51000-044) and the National Institutes of Health (K01-RR-024173, RO1-DK-54450). NR 59 TC 31 Z9 31 U1 1 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUL PY 2010 VL 299 IS 1 BP E69 EP E79 DI 10.1152/ajpendo.00080.2010 PG 11 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 609MH UT WOS:000278660200011 PM 20407005 ER PT J AU MacRoberts, MH MacRoberts, BR Rudolph, DC AF MacRoberts, Michael H. MacRoberts, Barbara R. Rudolph, D. Craig TI Vertebrate Richness and Biogeography in the Big Thicket of Texas SO AMERICAN MIDLAND NATURALIST LA English DT Article ID PLANT-SPECIES RICHNESS; EAST TEXAS; PATTERNS; DENSITY; RARE AB The Big Thicket of Texas has been described as rich in species and a "crossroads:" a place where organisms from many different regions meet. We examine the species richness and regional affiliations of Big Thicket vertebrates. We found that the Big Thicket is neither exceptionally rich in vertebrates nor is it a crossroads for vertebrates. Its vertebrate fauna is predominantly eastern. C1 [MacRoberts, Michael H.; MacRoberts, Barbara R.] Louisiana State Univ, Museum Life Sci, Herbarium, Shreveport, LA 71115 USA. [Rudolph, D. Craig] Stephen F Austin State Univ, Wildlife Habitat & Silviculture Lab, Coll Forestry, So Res Stn,USDA Forest Serv, Nacogdoches, TX 75962 USA. RP MacRoberts, MH (reprint author), Louisiana State Univ, Museum Life Sci, Herbarium, Shreveport, LA 71115 USA. EM mmacrobe@lsus.edu NR 52 TC 0 Z9 0 U1 0 U2 0 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 J9 AM MIDL NAT JI Am. Midl. Nat. PD JUL PY 2010 VL 164 IS 1 BP 37 EP 43 PG 7 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 619FV UT WOS:000279415500004 ER PT J AU Hagy, HM Linz, GM Bleier, WJ AF Hagy, Heath M. Linz, George M. Bleier, William J. TI Wildlife Conservation Sunflower Plots and Croplands as Fall Habitat for Migratory Birds SO AMERICAN MIDLAND NATURALIST LA English DT Article ID AGRICULTURAL LANDSCAPE; COMMERCIAL SUNFLOWER; BLACKBIRD DAMAGE; ABUNDANCE; FIELDS; HETEROGENEITY; FRAMEWORK; SELECTION; PATTERNS; WETLANDS AB Agricultural fields are often overlooked as post-breeding and migratory bird habitat, even though many species use row-crop fields in the northern Great Plains. We monitored bird use, crop and non-crop vegetation characteristics and abundance, and land use around (<= 2.4 km) 35 8-ha Wildlife Conservation Sunflower Plots (WCSP) and one commercial sunflower and non-sunflower row-crop field, paired with each WCSP, in fall 2004. and 2005. We excluded four species of blackbirds from our analysis that commonly form large foraging flocks and ubiquitously use agricultural fields. A diverse community of migratory birds used WCSP and commercial sunflower compared to other non-sunflower crops in North Dakota. Both WCSP (mean = 24.4 birds/ha, SE = 2.7) and commercial sunflower (mean = 12.7 birds/ha, SE = 1.7) harbored greater densities of birds (P < 0.01) than did other non-sunflower row crops (mean = 7.2 birds/ha, SE = 1.1). Migratory birds were more strongly associated with vegetation within fields, such as crop density (+), non-crop plant abundance (+) and crop height (+), than surrounding land uses (0-2.4 km from WCSP). We recommend management practices to maximize WCSP for fall bird habitat and discuss economic considerations for WCSP implementation as a wildlife habitat / blackbird damage management system. C1 [Hagy, Heath M.; Bleier, William J.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58105 USA. [Linz, George M.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. RP Hagy, HM (reprint author), Mississippi State Univ, Dept Wildlife & Fisheries, Box 9690, Mississippi State, MS 39762 USA. EM hhagy@cfr.msstate.edu FU United States Department of Agriculture Wildlife Service's National Wildlife Research Center; North Dakota Wildlife Services; North Dakota State University Department of Biological Sciences FX We thank the United States Department of Agriculture Wildlife Service's National Wildlife Research Center, North Dakota Wildlife Services and North Dakota State University Department of Biological Sciences for funding and support. We thank NDSU graduate students and field and laboratory assistants for their support and dedicated service. We thank S. K. Riffell for suggestions pertaining to our statistical analysis and R. M. Bush, G. M. Forcey, E. H. Hagy, M. McConnell and M. L. Schummer for evaluating early versions of this manuscript. NR 60 TC 3 Z9 3 U1 2 U2 5 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 EI 1938-4238 J9 AM MIDL NAT JI Am. Midl. Nat. PD JUL PY 2010 VL 164 IS 1 BP 119 EP 135 PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 619FV UT WOS:000279415500012 ER PT J AU Huang, YD Houston, NL Tovar-Mendez, A Stevenson, SE Miernyk, JA Randall, DD Thelen, JJ AF Huang, Yadong Houston, Norma L. Tovar-Mendez, Alejandro Stevenson, Severin E. Miernyk, Jan A. Randall, Douglas D. Thelen, Jay J. TI A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity SO ANALYTICAL BIOCHEMISTRY LA English DT Article DE CPK3; Mass spectrometry; Method; PDK; Peptide kinase assay; Protein phosphorylation ID PYRUVATE-DEHYDROGENASE KINASE; ELECTRON-TRANSFER DISSOCIATION; COLLISION-INDUCED DISSOCIATION; SYNTHETIC PEPTIDES; GLOBAL ANALYSIS; IN-VIVO; PHOSPHORYLATION; IDENTIFICATION; ARABIDOPSIS; PHOSPHOPROTEOME AB The Homo sapiens and Arabidopsis thaliana genomes are believed to encode more than 500 and 1000 protein kinases, respectively. Despite this abundance, few bona fide kinase-client relationships have been described in detail. Here we describe a quantitative mass spectrometry (MS)-based approach for identifying kinase-client proteins. During method development, we used the dedicated kinase pyruvate dehydrogenase kinase (PDK) for the in vitro assays. As kinase substrate, we used synthetic peptide cocktails and, in the process, demonstrated that the assay is both sensitive and specific. The method is also useful for characterizing protein kinase-substrate kinetics once the peptide substrate is detected. Applying a label-free spectral counting method, the activity of PDK was determined using the peptide substrate YHGH(292)SMSDPGSTYR derived from the pyruvate dehydrogenase E1 alpha subunit sequence. The utility of spectral counting was further validated by studying the negative effect of Met oxidation on peptide phosphorylation. We also measured the activity of the unrelated calcium-dependent protein kinase 3 (CPK3), demonstrating the utility of the method in protein kinase screening applications. (C) 2010 Elsevier Inc. All rights reserved. C1 [Huang, Yadong; Houston, Norma L.; Tovar-Mendez, Alejandro; Stevenson, Severin E.; Miernyk, Jan A.; Randall, Douglas D.; Thelen, Jay J.] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. [Huang, Yadong; Houston, Norma L.; Tovar-Mendez, Alejandro; Stevenson, Severin E.; Miernyk, Jan A.; Randall, Douglas D.; Thelen, Jay J.] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO 65211 USA. [Miernyk, Jan A.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. RP Thelen, JJ (reprint author), Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. EM thelenj@missouri.edu FU National Science Foundation (NSF) [DBI-0332418, DBI-0604439] FX We thank Jianjiong Gao (Digital Biology Lab, University of Missouri-Columbia) for writing the PhosSite script (available for download at digbio.missouri.edu/p3db/tools) and Beverly DaGue (Proteomics Center, University of Missouri-Columbia) for the MALDI-TOF analyses. This work was funded by National Science Foundation (NSF) Plant Genome Research Program Young Investigator award DBI-0332418 and DBI-0604439. NR 44 TC 18 Z9 19 U1 1 U2 7 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD JUL 1 PY 2010 VL 402 IS 1 BP 69 EP 76 DI 10.1016/j.ab.2010.03.028 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 601QG UT WOS:000278077200010 PM 20346904 ER PT J AU Connor, EE Li, RW Baldwin, RL Li, C AF Connor, E. E. Li, R. W. Baldwin, R. L. Li, C. TI Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes SO ANIMAL LA English DT Article; Proceedings Paper CT 11th International Symposium on Ruminant Physiology CY SEP 06-09, 2009 CL Clermont Ferrand, FRANCE DE development; diet; digestion; gene expression; parasitic disease ID CHAIN FATTY-ACIDS; HEALTHY DAIRY-COWS; 5-HYDROXYTRYPTAMINE RECEPTOR SUBTYPES; GASTROINTESTINAL MUSCLE LAYERS; CECAL DILATATION-DISLOCATION; GLUCOSE COTRANSPORTER SGLT1; MESSENGER-RIBONUCLEIC-ACID; SMALL-INTESTINAL EPITHELIA; TIME RT-PCR; NEONATAL CALVES AB The gastrointestinal tract (GIT) has multiple functions including digestion, nutrient absorption, secretion of hormones and excretion of wastes. In the ruminant animal, development of this organ system is more complex than that of the monogastric animal due to the necessity to establish a fully functional and differentiated rumen, in which a diverse microbial population of bacteria, fungi and protozoa support fermentation and digestion of dietary fiber. Central to the goal of animal scientists to enhance nutrient uptake and production efficiency of ruminants is the need for a comprehensive understanding of GIT development, as well as conditions that alter the digestion process. The relatively recent availability of genome sequence information has permitted physiological investigations related to the process of digestion for many agriculturally important species at the gene transcript level. For instance, numerous studies have evaluated the expression of ruminant GIT genes to gain insight into mechanisms involved in normal function, physiology and development, such as nutrient uptake and transport across the epithelial cell barrier throughout the alimentary canal, maintenance of rumen pH, and regulation of GIT motility and cell proliferation. Further, multiple studies have examined the effects of dietary modification, including feeding of supplemental fat, starch and protein, or a forage- v. concentrate-based diet on expression of critical gene pathways in the gut. In addition, the expression of genes in the GIT in response to disease, such as infection with gastrointestinal parasites, has been investigated. This review will summarize some of the recent scientific literature related to the gene expression in the GIT of ruminants, primarily cattle, sheep and goats, as it pertains to normal physiology, and dietary, developmental, and disease effects to provide an overview of critical proteins participating in the overall digestive processes, and their physiological functions. Recent findings from our laboratory will be highlighted also related to expression of the glucagon-like peptide two-hormone pathway in the GIT of dairy cattle during in various stages of the development and lactation, alterations in gene pathways associated with the rumen development and differentiation in the weaning calf, and genes/of the GIT responding to Ostertagia, a common nematode infection of the cattle. Finally, prospective areas of investigation will be highlighted. C1 [Connor, E. E.; Li, R. W.; Baldwin, R. L.; Li, C.] USDA ARS, Bovine Funct Genom Lab, Beltsville, MD USA. RP Connor, EE (reprint author), USDA ARS, Bovine Funct Genom Lab, Beltsville, MD USA. EM erin.connor@ars.usda.gov NR 88 TC 30 Z9 31 U1 2 U2 27 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 J9 ANIMAL JI Animal PD JUL PY 2010 VL 4 IS 7 BP 993 EP 1007 DI 10.1017/S1751731109991285 PG 15 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 615GZ UT WOS:000279124100003 PM 22444605 ER PT J AU Talbot, NC Powell, AM Caperna, TJ Garrett, WM AF Talbot, Neil C. Powell, Anne M. Caperna, Thomas J. Garrett, Wesley M. TI Proteomic analysis of the major cellular proteins of bovine trophectoderm cell lines derived from IVP, parthenogenetic and nuclear transfer embryos: Reduced expression of annexins I and II in nuclear transfer-derived cell lines SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Bovine; Trophectoderm; Cell; Proteomics ID CYTOSOLIC PHOSPHOLIPASE A(2); TISSUE-PLASMINOGEN-ACTIVATOR; CLONED TRANSGENIC CALVES; HUMAN FETAL MEMBRANES; PREIMPLANTATION EMBRYOS; PLACENTAL MEMBRANES; FUNCTIONAL PROTEOMICS; GENE-EXPRESSION; INTERFERON-TAU; HUMAN AMNION AB Trophectoderm cell lines were established from 8-day in vitro-cultured embryos of cattle derived from fertilization (IVF), somatic cell nuclear transfer (NT), or parthenogenetic activation (P) of in vitro-matured oocytes and from five 8-day-old in vivo (V) embryos. The most abundant cellular proteins of 5 V-, 16 NT-, 12 P-, and 16 IVF-derived cell lines were compared by 2D-gel electrophoresis and mass spectrometry; that is, the unaltered thiourea/urea extract of each cell culture was analyzed. Common protein spots (n=118) were examined, and 95% were identified with significant scores from protein and gene database searches. Of the proteins detected and identified, actin and cytokeratin-8 were found to be the most abundant. Other prominent cellular proteins were metabolic enzymes such as aldose reductase, phosphoglycerate mutase, enolase, triosephosphate isomerase, cytoskeletal interacting proteins transgelin and stratifin, anti-oxidant proteins peroxiredoxin 1 and anti-oxidant protein 2, and the calcium-dependent lipid-binding proteins annexins I and II. In comparative analysis of the 2D-gels, the NT-derived trophectoderm had less annexins I and II in comparison to the IVF- and P-derived trophectoderm. Because annexins I and II are abundant in the placenta and have functions important to the maintenance of placentation, the down-regulation of the annexin genes in the cultured NT trophectoderm may be related to the frequent failures of NT pregnancies. Published by Elsevier B.V. C1 [Talbot, Neil C.; Powell, Anne M.; Caperna, Thomas J.; Garrett, Wesley M.] ARS, USDA, ANRI, ABBL,Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Talbot, NC (reprint author), ARS, USDA, ANRI, ABBL,BARC E, Bldg 200,Rm 13, Beltsville, MD 20705 USA. EM neil.talbot@ars.usda.gov NR 61 TC 6 Z9 8 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD JUL PY 2010 VL 120 IS 1-4 BP 187 EP 202 DI 10.1016/j.anireprosci.2010.03.009 PG 16 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA 609QQ UT WOS:000278672600026 PM 20400246 ER PT J AU Keller, M Tarara, JM AF Keller, Markus Tarara, Julie M. TI Warm spring temperatures induce persistent season-long changes in shoot development in grapevines SO ANNALS OF BOTANY LA English DT Article DE Apical dominance; budbreak; grapevine; growth; leaf expansion; shoot elongation; temperature; Vitis vinifera ID VITIS-VINIFERA L; AXILLARY BUD OUTGROWTH; TROPHIC COMPETITION; LIGHT INTERCEPTION; INTRA-SHOOT; GROWTH; LEAF; ARABIDOPSIS; CULTIVARS; PHOTOSYNTHESIS AB The influence of temperature on the timing of budbreak in woody perennials is well known, but its effect on subsequent shoot growth and architecture has received little attention because it is understood that growth is determined by current temperature. Seasonal shoot development of grapevines (Vitis vinifera) was evaluated following differences in temperature near budbreak while minimizing the effects of other microclimatic variables. Dormant buds and emerging shoots of field-grown grapevines were heated above or cooled below the temperature of ambient buds from before budbreak until individual flowers were visible on inflorescences, at which stage the shoots had four to eight unfolded leaves. Multiple treatments were imposed randomly on individual plants and replicated across plants. Shoot growth and development were monitored during two growing seasons. Higher bud temperatures advanced the date of budbreak and accelerated shoot growth and leaf area development. Differences were due to higher rates of shoot elongation, leaf appearance, leaf-area expansion and axillary-bud outgrowth. Although shoots arising from heated buds grew most vigorously, apical dominance in these shoots was reduced, as their axillary buds broke earlier and gave rise to more vigorous lateral shoots. In contrast, axillary-bud outgrowth was minimal on the slow-growing shoots emerging from buds cooled below ambient. Variation in shoot development persisted or increased during the growing season, well after temperature treatments were terminated and despite an imposed soil water deficit. The data indicate that bud-level differences in budbreak temperature may lead to marked differences in shoot growth, shoot architecture and leaf-area development that are maintained or amplified during the growing season. Although growth rates commonly are understood to reflect current temperatures, these results demonstrate a persistent effect of early-season temperatures, which should be considered in future growth models. C1 [Keller, Markus] Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Hort & Landscape Architecture, Prosser, WA 99350 USA. [Tarara, Julie M.] ARS, Hort Crops Res Unit, USDA, Prosser, WA 99350 USA. RP Keller, M (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Hort & Landscape Architecture, Prosser, WA 99350 USA. EM mkeller@wsu.edu FU US Department of Agriculture-Agricultural Research Service (USDA-ARS) Northwest Center; USDA-ARS CRIS [5358-21000-034-D] FX This work was supported by a grant from the US Department of Agriculture-Agricultural Research Service (USDA-ARS) Northwest Center for Small Fruits Research and by funds from USDA-ARS CRIS project no. 5358-21000-034-D. We thank John Ferguson for the design and technical support of the temperature-control system, Lynn Mills and Babette Grunwald for assistance with field and laboratory measurements, and Carolyn Scagel for statistical advice. NR 45 TC 13 Z9 13 U1 1 U2 29 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7364 EI 1095-8290 J9 ANN BOT-LONDON JI Ann. Bot. PD JUL PY 2010 VL 106 IS 1 BP 131 EP 141 DI 10.1093/aob/mcq091 PG 11 WC Plant Sciences SC Plant Sciences GA 616CD UT WOS:000279184600010 PM 20513742 ER PT J AU Kim, H Hoelmer, KA Lee, W Kwon, YD Lee, S AF Kim, Hyojoong Hoelmer, Kim A. Lee, Wonhoon Kwon, Young-Dae Lee, Seunghwan TI Molecular and Morphological Identification of the Soybean Aphid and Other Aphis Species on the Primary Host Rhamnus davurica in Asia SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Aphis glycines; Aphis gossypii; host alternation; molecular identification; morphology ID INSECTA HEMIPTERA APHIDIDAE; MITOCHONDRIAL-DNA SEQUENCES; GOSSYPII GLOVER; REPRODUCTIVE ISOLATION; GENETIC-DIVERGENCE; GLYCINES MATSUMURA; EVOLUTION; PHYLOGENY; HOMOPTERA; GENUS AB The soybean aphid, Aphis glycines Matsumura, was recently introduced into North America where it has become a serious pest of soybean, Glyine max (L.) Merr. In its native range of northeastern Asia, A. glycines undergoes host alternation between the soybean (summer host) and Dahurian buckthorn, Rhamnus davurica Pallas (winter host). On the primary host, it is difficult to discriminate A. glycines from coexisting, morphologically similar Aphis species: including seasonal polymorphisms of each species (e.g., gynopara, ovipara, and male). Two widely used molecular markers, the mitochondrial cytochrome c oxidase I (COI) "barcode" region (658 bp) and the partial tRNA-leucine + cytochrome c oxidase H (tRNA/COII, 702 bp), were used to analyze 31 individuals of Aphis from R. davurica in Asia and compared with 26 closely related Aphis species. We found that three different species, Aphis gossypii Glover and two new and undescribed putative Aphis species, occur together with A. glycines on R. davurica. All these species were genetically close within the gossypii group. A study of 28 quantitative morphological characters showed that A. glycines, A. gossypii, and one of the new species were quite similar with only a few characters differing significantly between species. C1 [Kim, Hyojoong; Lee, Wonhoon; Lee, Seunghwan] Seoul Natl Univ, Dept Agr Biotechnol, Res Inst Agr & Life Sci, Seoul 151921, South Korea. [Hoelmer, Kim A.] USDA ARS, Beneficial Insect Intro Res Unit, Newark, DE 19711 USA. [Kwon, Young-Dae] Gyeonggi Do Forestry Environm Res Inst, Osan 447290, South Korea. RP Lee, S (reprint author), Seoul Natl Univ, Dept Agr Biotechnol, Res Inst Agr & Life Sci, Seoul 151921, South Korea. EM seung@snu.ac.kr FU Technology Development Program for Agriculture and Forestry; Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea [500-20080241]; National Institute of Biological Resources under the Ministry of Environment, Korea; USDA-ARS [58-1926-7-154 F] FX We are indebted to Robert Foottit, Eric Maw, Jaroslav Holman, Jan Havelka for the loan of Aphis specimens from their regions for comparison. We thank Gexia Qiao, Liyun Jiang, and Xiaolei Huang for cooperative aphid collection in the Changbaishan area in northeastern China. We also thank Yoshiyuki Matsumoto for sending Japanese samples. Special thanks to Youngboon Lee (Seoul National University) for preparing many aphid specimens, to Wonyeol Jang for assistance with the measurement of specimens, and to Yeyeun Kim for assistance with the molecular work. This research was supported by the grants from the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (500-20080241) and the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources under the Ministry of Environment, Korea. H.K. and S.L. also were supported by USDA-ARS specific cooperative agreement 58-1926-7-154 F. NR 52 TC 18 Z9 19 U1 0 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2010 VL 103 IS 4 BP 532 EP 543 DI 10.1603/AN09166 PG 12 WC Entomology SC Entomology GA 621BJ UT WOS:000279548300008 ER PT J AU Wright, LC James, DG Reyna, V del Conte, SC Gingras, S Landolt, P Brooks, T AF Wright, Lawrence C. James, David G. Reyna, Victor del Conte, Sandra Castle Gingras, Stacey Landolt, Peter Brooks, Tora TI Species Composition of Cutworm (Lepidoptera: Noctuidae) Larvae in South Central Washington Vineyards SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE cutworm; grape; Noctuidae; Abagrotis orbis; Agrotis vetusta ID CLIMBING CUTWORMS AB The major grape (Vitis spp.)-growing areas of Washington were surveyed during 2003-2007 to determine the cutworm species present as larvae in vineyards during spring when grape buds and young shoots were vulnerable to cutworm (Lepidoptera: Noctuidae) damage. We sampled vineyard floors during daytime, vines at night because cutworms are active at night, and vines during the day. In total, 1,003 larvae were collected and 650 were reared to adults and identified. Twenty-five species were found: 22 on the ground, eight on vines at night, and two on vines during the day. Almost 75% of the cutworms on vines at night were Abagrotis orbis (Grote) and 19% were Agrotis vetusta Walker. The spotted cutworm, Xestia c-nigrum (L.), and the redbacked cutworm, Euxoa ochrogaster (Guenee), were reported previously to be the major cutworm pests of grapes in Washington, but only four X. c-nigrum and no E. ochrogaster were collected. Abagrotis orbis larvae were collected on grapes at night from mid-March to late April. Adults emerged in the laboratory in mid-May, and they were present during the summer. Agrotis vetusta larvae were collected on grapes from 1 to 30 April. This species had a long prepupal period during the summer, followed by a pupal stadium of approximate to 3 wk. Mean adult emergence was in early August. Both species were univoltine, with mating and egg laying during the fall followed by overwintering as small larvae. C1 [Wright, Lawrence C.; James, David G.; Reyna, Victor; del Conte, Sandra Castle; Gingras, Stacey; Brooks, Tora] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Landolt, Peter] USDA ARS, Wapato, WA 98951 USA. RP Wright, LC (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM lawrence_wright@wsu.edu FU Washington State Concord Grape Research Council; Washington Wine Advisory Council; Northwest Center for Small Fruits Research FX We thank the many grape growers who allowed us to sample their vineyards and Jesse Fullbright for technical assistance. We appreciate the work of the following taxonomists who identified the parasitoids: Norman E. Woodley (Diptera) and Robert Kula (Hymenoptera), Systematic Entomology Laboratory, USDA-ARS. This work was supported in part by funding from the Washington State Concord Grape Research Council, the Washington Wine Advisory Council, and the Northwest Center for Small Fruits Research. NR 15 TC 1 Z9 1 U1 2 U2 7 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2010 VL 103 IS 4 BP 592 EP 596 DI 10.1603/AN09112 PG 5 WC Entomology SC Entomology GA 621BJ UT WOS:000279548300016 ER PT J AU Hall, DG Shatters, RG Carpenter, JE Shapiro, JP AF Hall, David G. Shatters, Robert G. Carpenter, James E. Shapiro, Jeffrey P. TI Research Toward an Artificial Diet for Adult Asian Citrus Psyllid SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE citrus greening disease; huanglongbing; Diaphorina citri; rearing ID BEMISIA-ARGENTIFOLII HOMOPTERA; DIAPETIMORPHA-INTROITA; ACYRTHOSIPHON-PISUM; PEA APHID; ALEYRODIDAE; BIOLOGY; DISEASE; SYSTEM AB Research progress is reported on an artificial diet for adult Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). The primary objective was to develop a system for screening antimicrobial peptides and other potential toxic proteins for activity against adults. The base diet was a sterilized solution of sucrose (30%) and yellow-green food coloring (0.5%) in tap water. All of the studies presented were conducted at 25 degrees C, 75% RH, and a photoperiod of 14:10 (L:D) h. Adult psyllids were <7 d old when they were transferred to diet. Addition of the food coloring was necessary to prompt adults to feed. Among the feeding trials discussed, a mean of 69.1 +/- 3.2% adults survived for 14 d on the base sucrose diet. Survival rates of males and females were similar. Adults feeding on the sucrose diet may have ingested less food than adults feeding on citrus leaf disks based on differences in quantities of adult excrements deposited in feeding chambers. However, survival of adults feeding on leaf disks over a 2-wk period was only marginally better than survival of adults feeding on the base sucrose diet, and final rates of survival of adults fed these two food sources were not significantly different. C1 [Hall, David G.; Shatters, Robert G.] USDA ARS, Ft Pierce, FL 34945 USA. [Carpenter, James E.] USDA ARS, Tifton, GA 31793 USA. [Shapiro, Jeffrey P.] USDA ARS, Gainesville, FL 32608 USA. RP Hall, DG (reprint author), USDA ARS, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM david.hall@ars.usda.gov NR 21 TC 13 Z9 13 U1 3 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2010 VL 103 IS 4 BP 611 EP 617 DI 10.1603/AN10004 PG 7 WC Entomology SC Entomology GA 621BJ UT WOS:000279548300019 ER PT J AU Lounsberry, Z Spiewok, S Pernal, SF Sonstegard, TS Hood, WM Pettis, J Neumann, P Evans, JD AF Lounsberry, Zachary Spiewok, Sebastian Pernal, Stephen F. Sonstegard, Tad S. Hood, W. Michael Pettis, Jeffery Neumann, Peter Evans, Jay D. TI Worldwide Diaspora of Aethina tumida (Coleoptera: Nitidulidae), a Nest Parasite of Honey Bees SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE invasive species; DNA barcode; Aethina tumida; Apis mellifera; pollination ID SMALL HIVE BEETLE; POPULATION-STRUCTURE; BOMBUS-IMPATIENS; COLONIES; HYMENOPTERA; INFESTATION; BIOLOGY; MURRAY; APIDAE; PEST AB Native to sub-Saharan Africa, Aethina tumida Murray (Coleoptera: Nitidulidae) is now an invasive pest of honey bee, Apis mellifera L., colonies in Australia and North America. Knowledge about the introduction (s) of this beetle from Africa into and among the current ranges will elucidate pest populations and invasion pathways and contribute to knowledge of how a parasite expands in new populations. We examined genetic variation in adult beetle samples from the United States, Australia, Canada, and Africa by sequencing a 912-base pair region of the mitochondrial DNA cytochrome c oxidase subunit I gene and screening 10 informative microsatellite loci. One Canadian introduction of small hive beetles can be traced to Australia, whereas the second introduction seems to have come from the United States. Beetles now resident in Australia were of a different African origin than were beetles in North America. North American beetles did not show covariance between two mitochondrial haplotypes and their microsatellite frequencies, suggesting that these beetles have a shared source despite having initial genetic structure within their introduced range. Excellent dispersal of beetles, aided in some cases by migratory beekeeping and the bee trade, seems to lead to panmixis in the introduced populations as well as in Africa. C1 [Lounsberry, Zachary; Pettis, Jeffery; Evans, Jay D.] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Spiewok, Sebastian] Deutsch Bauernverlag GmbH, D-10713 Berlin, Germany. [Pernal, Stephen F.] Agr & Agri Food Canada, Beaverlodge, AB T0H 0O0, Canada. [Sonstegard, Tad S.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Hood, W. Michael] Clemson Univ, Dept Entomol, Clemson, SC 29634 USA. [Neumann, Peter] Swiss Bee Res Ctr, Agroscope Liebefeld Posieux Res Stn ALP, CH-3033 Bern, Switzerland. [Neumann, Peter] Rhodes Univ, Dept Zool & Entomol, ZA-6140 Grahamstown, South Africa. [Neumann, Peter] Yunnan Agr Univ, Eastern Bee Res Inst, Kunming, Yunnan Province, Peoples R China. RP Evans, JD (reprint author), ARS, USDA, Bee Res Lab, BARC E Bldg 476, Beltsville, MD 20705 USA. EM jay.evans@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 NR 37 TC 8 Z9 8 U1 2 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2010 VL 103 IS 4 BP 671 EP 677 DI 10.1603/AN10027 PG 7 WC Entomology SC Entomology GA 621BJ UT WOS:000279548300026 ER PT J AU French, BW Hammack, L AF French, B. Wade Hammack, Leslie TI Reproductive Traits of Northern Corn Rootworm (Coleoptera: Chrysomelidae) in Relation to Female and Male Body Size SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE mate choice; fecundity; longevity; Diabrotica barberi ID DIABROTICA-VIRGIFERA-VIRGIFERA; BACILLUS-THURINGIENSIS; INSECT RESISTANCE; SEXUAL SELECTION; MATE CHOICE; BEETLE COLEOPTERA; MATING-BEHAVIOR; TRANSGENIC CORN; WESTERN; SURVIVAL AB Diabrotica spp. are serious pests of maize, Zea mays L., and have evolved resistance to cultural and chemical management practices. Transgenic maize producing a rootworm-toxic protein derived from the bacterium Bacillus thuringiensis Berliner provides a new approach to Diabrotica spp. management. Its use requires a refuge of nontransgenic maize to produce susceptible beetles able to mate with any resistant individuals developing on the transgenic variety to slow the evolution of resistance. Such evolution may simultaneously affect fitness related traits such as longevity, fecundity, and body size. We examined the mating behavior and reproductive biology of Diabrotica barberi Smith & Lawrence in relation to male and female body size. Large and small males were reciprocally paired to large and small females for comparison with average sized beetles. Neither the proportion of pairs that copulated nor the precopulation duration varied significantly with size category; however, the duration of copulation was shortest for large males crossed with small females and longest for the reciprocal cross. Large females lived the longest regardless of mating partner. Although small females mated to small males tended to lay the fewest eggs, size category did not significantly affect egg numbers per week of life during a 12-wk oviposition period. Egg production peaked during the first 4 to 5 wk and then steadily declined. These results suggest that resistance to transgenic maize, if associated with small body size, could provide a reproductive disadvantage for resistant females but not males that could prolong efficacy of the transgenic maize. C1 [French, B. Wade] ARS, N Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. RP French, BW (reprint author), ARS, N Cent Agr Res Lab, USDA, 2923 Medary Ave, Brookings, SD 57006 USA. EM wade.french@ars.usda.gov NR 55 TC 11 Z9 12 U1 1 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2010 VL 103 IS 4 BP 688 EP 694 DI 10.1603/AN09174 PG 7 WC Entomology SC Entomology GA 621BJ UT WOS:000279548300029 ER PT J AU Fazier, M Muli, E Conklin, T Schmehl, D Torto, B Frazier, J Tumlinson, J Evans, JD Raina, S AF Fazier, Maryann Muli, Eliud Conklin, Tracy Schmehl, Daniel Torto, Baldwyn Frazier, James Tumlinson, James Evans, Jay D. Raina, Suresh TI A scientific note on Varroa destructor found in East Africa; threat or opportunity? SO APIDOLOGIE LA English DT Article DE Varroa destructor; Apis mellifera scutellata; Apis mellifera monticola; hygienic behavior ID HONEY-BEES HYMENOPTERA; HYGIENIC BEHAVIOR; AMERICAN FOULBROOD; APIS-MELLIFERA; RESISTANCE; DISEASE; APIDAE C1 [Fazier, Maryann; Conklin, Tracy; Schmehl, Daniel; Frazier, James; Tumlinson, James] Penn State, Dept Entomol, Ctr Chem Ecol, University Pk, PA 16802 USA. [Muli, Eliud; Torto, Baldwyn; Raina, Suresh] Int Ctr Insect Physiol & Ecol, Nairobi, Kenya. [Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD USA. RP Fazier, M (reprint author), Penn State, Dept Entomol, Ctr Chem Ecol, 501 ASI Bldg, University Pk, PA 16802 USA. EM mfrazier@psu.edu RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU USDA; IFAD FX This project was funded by a grant from USDA CSREES-ISEC and with additional funds from icipe and IFAD. We would also like to thank beekeeping technicians James Nganga Kimani, Joseph Kilonzo Wambua and Tom McCormack, who assisted with the sample collection and hygienic behavior assays and Kenyan beekeepers who allowed us to sample colonies in their respective apiaries. NR 12 TC 14 Z9 15 U1 0 U2 5 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 J9 APIDOLOGIE JI Apidologie PD JUL-AUG PY 2010 VL 41 IS 4 BP 463 EP 465 DI 10.1051/apido/2009073 PG 3 WC Entomology SC Entomology GA 630RL UT WOS:000280290600007 ER PT J AU Lorio, JC Kim, WS Krishnan, AH Krishnan, HB AF Lorio, Julio C. Kim, Won-Seok Krishnan, Ammulu H. Krishnan, Hari B. TI Disruption of the Glycine Cleavage System Enables Sinorhizobium fredii USDA257 To Form Nitrogen-Fixing Nodules on Agronomically Improved North American Soybean Cultivars SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID SYMBIONT RHIZOBIUM-FREDII; BRADYRHIZOBIUM-JAPONICUM; HOST-RANGE; SECRETION SYSTEMS; ESCHERICHIA-COLI; STRAIN NGR234; NODULATION; GENOME; SPECIFICITY; GENE AB The symbiosis between Sinorhizobium fredii USDA257 and soybean [Glycine max (L.) Merr.] exhibits a high degree of cultivar specificity. USDA257 nodulates primitive soybean cultivars but fails to nodulate agronomically improved cultivars such as McCall. In this study we provide evidence for the involvement of a new genetic locus that controls soybean cultivar specificity. This locus was identified in USDA257 by Tn5 transposon mutagenesis followed by nodulation screening on McCall soybean. We have cloned the region corresponding to the site of Tn5 insertion and found that it lies within a 1.5-kb EcoRI fragment. DNA sequence analysis of this fragment and an adjacent 4.4-kb region identified an operon made up of three open reading frames encoding proteins of deduced molecular masses of 41, 13, and 104 kDa, respectively. These proteins revealed significant amino acid homology to glycine cleavage (gcv) system T, H, and P proteins of Escherichia coli and other organisms. Southern blot analysis revealed the presence of similar sequences in diverse rhizobia. Measurement of beta-galactosidase activity of a USDA257 strain containing a transcriptional fusion of gcvT promoter sequences to the lacZ gene revealed that the USDA257 gcvTHP operon was inducible by glycine. Inactivation of either gcvT or gcvP of USDA257 enabled the mutant to nodulate several agronomically improved North American soybean cultivars. These nodules revealed anatomical features typical of determinate nodules, with numerous bacteroids within the infected cells. Unlike for the previously characterized soybean cultivar specificity locus nolBTUVW, inactivation of the gcv locus had no discernible effect on the secretion of nodulation outer proteins of USDA257. C1 [Krishnan, Hari B.] Univ Missouri, ARS, USDA, Plant Genet Res Unit, Columbia, MO 65211 USA. [Lorio, Julio C.; Kim, Won-Seok; Krishnan, Ammulu H.; Krishnan, Hari B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), Univ Missouri, ARS, USDA, Plant Genet Res Unit, 108 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@ARS.USDA.GOV NR 50 TC 4 Z9 4 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2010 VL 76 IS 13 BP 4185 EP 4193 DI 10.1128/AEM.00437-10 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 614TN UT WOS:000279082800009 PM 20453144 ER PT J AU Durso, LM Harhay, GP Smith, TPL Bono, JL DeSantis, TZ Harhay, DM Andersen, GL Keen, JE Laegreid, WW Clawson, ML AF Durso, Lisa M. Harhay, Gregory P. Smith, Timothy P. L. Bono, James L. DeSantis, Todd Z. Harhay, Dayna M. Andersen, Gary L. Keen, James E. Laegreid, William W. Clawson, Michael L. TI Animal-to-Animal Variation in Fecal Microbial Diversity among Beef Cattle SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RIBOSOMAL-RNA SEQUENCES; SPECIES RICHNESS; PCR; TAXONOMY; PROGRAM; FLORA AB The intestinal microbiota of beef cattle are important for animal health, food safety, and methane emissions. This full-length sequencing survey of 11,171 16S rRNA genes reveals animal-to-animal variation in communities that cannot be attributed to breed, gender, diet, age, or weather. Beef communities differ from those of dairy. Core bovine taxa are identified. C1 [Durso, Lisa M.; Harhay, Gregory P.; Smith, Timothy P. L.; Bono, James L.; Harhay, Dayna M.; Keen, James E.; Laegreid, William W.; Clawson, Michael L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [DeSantis, Todd Z.; Andersen, Gary L.] Univ Calif Berkeley, Lawrence Berkeley Lab, Ctr Environm Biotechnol, Berkeley, CA 94720 USA. RP Durso, LM (reprint author), ARS, USDA, ANRU, Room 307,Biochem Hall,UNL E Campus, Lincoln, NE 68583 USA. EM lisa.durso@ars.usda.gov RI Andersen, Gary/G-2792-2015; OI Andersen, Gary/0000-0002-1618-9827; Clawson, Michael/0000-0002-3355-5390 FU USDA, ARS [108] FX Support for this study was provided by the USDA, ARS, National Program 108. NR 19 TC 55 Z9 60 U1 3 U2 28 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2010 VL 76 IS 14 BP 4858 EP 4862 DI 10.1128/AEM.00207-10 PG 5 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 621UX UT WOS:000279611500033 PM 20472731 ER PT J AU Brummer, SP Gese, EM Shivik, JA AF Brummer, Stacey P. Gese, Eric M. Shivik, John A. TI The effect of enclosure type on the behavior and heart rate of captive coyotes SO APPLIED ANIMAL BEHAVIOUR SCIENCE LA English DT Article DE Coyote; Canis latrans; Enclosure; Behavior; Heart rate; Stress ID DIFFERENT COPING CHARACTERISTICS; FOXES VULPES-VULPES; WOLVES CANIS-LUPUS; STEREOTYPIC BEHAVIOR; HOUSING CONDITIONS; ENVIRONMENTAL ENRICHMENT; STRESS RESPONSES; GESTATING SOWS; ALOPEX-LAGOPUS; RED FOXES AB The potential for captivity to elicit changes in animal behavior and physiology is well known. Recent research on captive populations has examined the effect of feeding protocols, enclosure types, and enrichment programs on indices of stress and displays of species-typical behaviors. We investigated the impact of enclosure type upon captive coyotes (Canis latrans) by examining differences in coyote behavior and heart rate, among 3.3 m(2) kennels (K), 65.5 m(2) small pens (S), and 1000 m(2) large pens (L). Time budgets and repertoire of species-specific behaviors were compared among each enclosure type and to a sample of wild (W) coyotes. Baseline heart rates and heart rate (HR) responses to food delivery and fecal collection (measured as mean heart rate and latency of heart rate to return to baseline) were also compared among treatments. We found that behavioral budgeting, but not repertoire, differed significantly among enclosure types. Relative to small and large pen enclosures, coyotes maintained in kennels exhibited the greatest amount of stereotypic behavior (P < 0.0001). Coyotes kept in large pens were most similar to wild coyotes in the percentage of time they spent performing exploratory (K: 2.7%; S: 4.9%; L: 8.5%; W: 12.0%) and stand and scan (K: 8.0%; S: 16.4%; L: 22.0%; W: 22.3%) behaviors. Heart rate analysis showed that baseline heart rates and heart rate responses to food delivery did not differ significantly among enclosure types. Mean heart rate responses to fecal collection were significantly higher for kennel coyotes than for those maintained in large pens (P = 0.04). Similarly, latency to return to baseline was significantly higher in kennels than in small and large pens (P = 0.001). These results suggest that enclosure type does influence coyote behavior and heart rate responses to some human activities. Published by Elsevier B.V. C1 [Brummer, Stacey P.] Utah State Univ, USDA, Predator Project, APHIS,WS,NWRC, Logan, UT 84322 USA. RP Brummer, SP (reprint author), Utah State Univ, USDA, Predator Project, APHIS,WS,NWRC, BNR 163, Logan, UT 84322 USA. EM Stacey.P.Brummer@aphis.usda.gov RI Gese, Eric/B-4578-2011; Max, Mad/E-5238-2010 OI Max, Mad/0000-0001-6966-6829 FU National Wildlife Research Center FX This study was funded by the National Wildlife Research Center. We would like to thank the staff at the Wildlife Services National Wildlife Research Center's Predator Research Facility in Millville, UT, USA for their time, effort and help in this study. This study could not have been conducted without the help and support of Patrick Darrow, Jared Hedelius and Jeff Schultz. Thanks to Doris Zemlicka for her logistical support, and to Paige Groninger and Susan Durham for their statistical assistance. NR 52 TC 7 Z9 7 U1 10 U2 55 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1591 J9 APPL ANIM BEHAV SCI JI Appl. Anim. Behav. Sci. PD JUL PY 2010 VL 125 IS 3-4 BP 171 EP 180 DI 10.1016/j.applanim.2010.04.012 PG 10 WC Agriculture, Dairy & Animal Science; Behavioral Sciences; Veterinary Sciences SC Agriculture; Behavioral Sciences; Veterinary Sciences GA 626KN UT WOS:000279965000012 ER PT J AU Peterson, DL Tabb, AL Baugher, TA Lewis, K Glenn, DM AF Peterson, D. L. Tabb, A. L. Baugher, T. A. Lewis, K. Glenn, D. M. TI DRY BIN FILLER FOR APPLES SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Dry bin filler; Apples; Fruit; Quality; Fruit handling; Fruit bruising AB A unique dry bin filler for apples using a sequenced tray was developed to reduce bruising in packing operations. Research and commercial trials in West Virginia, Pennsylvania, and Washington State demonstrated the ability to fill bins evenly and with low damage. Cultivars with different bruising susceptibility ('Pink Lady,' Golden Delicious,' 'McIntosh,' 'Mutsu,' 'Delicious,' and 'Fuji') were used to test the bin filler in research trials and commercial assessments. Fruit that were downgraded from U.S. Extra Fancy grade after handling by the bin filler were 1.7%, 1.4%, and 2.9% at each test location. The filler was shown to produce less than 5% bruising on fruit and to have the ability to operate in commercial locations. C1 [Peterson, D. L.; Tabb, A. L.; Glenn, D. M.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Baugher, T. A.] Penn State Cooperat Extens, Gettysburg, PA USA. [Lewis, K.] Washington State Univ, Washington, DC USA. RP Tabb, AL (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM amy.tabb@ars.usda.gov NR 15 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2010 VL 26 IS 4 BP 541 EP 549 PG 9 WC Agricultural Engineering SC Agriculture GA 645JM UT WOS:000281451600002 ER PT J AU Loughrin, JH Bolster, CC Lovanh, NC Sistani, KR AF Loughrin, J. H. Bolster, C. C. Lovanh, N. C. Sistani, K. R. TI A SIMPLE DEVICE FOR THE COLLECTION OF WATER AND DISSOLVED GASES AT DEFINED DEPTHS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Depth; Lagoons; Lake; Odor; VOC; Wastewater; Water; Water quality ID DEGRADATION; LAGOON AB A device, consisting of a jar fitted with an inlet comprised of a gas-tight check valve and two-way ball valve outlet connected via tubing to a portable peristaltic pump, was constructed to collect water samples without atmospheric contamination or loss of dissolved gases. A headspace void for dissolved gas analysis was created by enclosing silicone tubing sealed with rubber stoppers within the jar. The device was deployed in a 0.4-ha lagoon that served as the primary waste recipient of a 2,000-sow farrowing operation as well as a 4,000-ha impoundment reservoir: Water samples were analyzed for dissolved methane and common indices of water quality such as pH, suspended solids, various ions, and metals concentration. In addition, lagoon wastewater samples were analyzed for malodorous compounds. For reservoir samples, methane concentrations at the bottom of the lake of 60 mu g L(-1) were about three orders of magnitude higher than at the upper levels of the lake while ammonium levels increased from 0.03 mg L(-1) at the surface to 1.67 mg L(-1) in bottom samples. pH steadily decreased from 8.58 near the surface to 7.47 at the bottom. Other water quality parameters such as dissolved metals were similar at each depth. For lagoon samples, ammonium concentration and malodorous compounds declined markedly as temperatures warmed. Results for both reservoir and lagoon sampling indicate that this device affords an inexpensive yet effective means of water collection for subsequent analysis of both water quality and dissolved gas concentration. C1 [Loughrin, J. H.; Bolster, C. C.; Lovanh, N. C.; Sistani, K. R.] USDA ARS, Anim Waste Management Res Unit, Bowling Green, KY 42104 USA. RP Loughrin, JH (reprint author), USDA ARS, Anim Waste Management Res Unit, 230 Bennett Lane, Bowling Green, KY 42104 USA. EM John.loughrin@ars.usda.gov NR 18 TC 4 Z9 4 U1 1 U2 9 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2010 VL 26 IS 4 BP 559 EP 564 PG 6 WC Agricultural Engineering SC Agriculture GA 645JM UT WOS:000281451600004 ER PT J AU Haff, RP Pearson, TC Toyofuku, N AF Haff, R. P. Pearson, T. C. Toyofuku, N. TI SORTING OF IN-SHELL PISTACHIO NUTS FROM KERNELS USING COLOR IMAGING SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Pistachio; Kernel; In-shell; Small in-shell; Discriminant analysis; K-nearest neighbor; Sorting; Processing stream ID BAND SELECTION; CONTAMINATION; DEFECTS AB Sorting pistachio kernels from in-shell nuts currently requires a combination of automated and manual sorting, an expensive and labor-intensive two-stage process. This research demonstrates the feasibility of using color imaging as a basis for distinguishing both regular and small in-shell pistachio nuts front kernels in the pistachio nut process stream. Two algorithms were developed to classify images of in-shell nuts, small in-shell nuts, and kernels. The first algorithm used a discriminant analysis (DA) routine to evaluate features extracted from the images based on histograms of red, green, and blue (RGB) pixel intensities, and resulted in a 99.9% overall accuracy for separating regular in-shell pistachio nuts front kernels. Small in-shell pistachio nuts were harder to discriminate from kernels, with an overall accuracy of 85%. The second algorithm used a k-nearest neighbors (knn) routine to evaluate features based on color histograms plus intensity slope information. The knn routine matched the accuracy of the DA algorithm for distinguishing regular in-shells from kernels with 99.9% correct. For the small in-shell case, however, the knit approach was superior with. 96% accuracy. When used in a high-speed color imaging system, the algorithms would provide the means for economical high-speed sorting of in-shell pistachio nuts and kernels. C1 [Haff, R. P.; Toyofuku, N.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Pearson, T. C.] USDA ARS, GMPRC, Manhattan, KS USA. RP Haff, RP (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM Ron.Haff@ars.usda.gov NR 18 TC 3 Z9 4 U1 0 U2 0 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2010 VL 26 IS 4 BP 633 EP 638 PG 6 WC Agricultural Engineering SC Agriculture GA 645JM UT WOS:000281451600010 ER PT J AU Price, RM Savabi, MR Jolicoeur, JL Roy, S AF Price, Rene M. Savabi, M. Reza Jolicoeur, Jean L. Roy, Srikumar TI Adsorption and desorption of phosphate on limestone in experiments simulating seawater intrusion SO APPLIED GEOCHEMISTRY LA English DT Article ID SOUTH FLORIDA; GROUNDWATER DISCHARGE; BISCAYNE AQUIFER; PHOSPHORUS; EVERGLADES; USA; DISSOLUTION; REDUCTION; WETLANDS; YUCATAN AB This study investigates the potential release of PO(4)(3-) from carbonate aquifers exposed to seawater intrusion. Adsorption and desorption of PO(4)(3-) in the presence of deionized water (DIW) and seawater were conducted on a large block of Pleistocene age limestone to simulate the effects of seawater intrusion into a coastal carbonate aquifer at the laboratory scale. The limestone showed strong adsorption of PO(4)(3-) in DIW, while adsorption was significantly less in the presence of seawater. Dissolution of CaCO(3) was found to prevent PO(4)(3-) adsorption at salinities less than 30 psu. Adsorption of PO(4)(3-) was limited at higher salinities (30-33 psu), due to competition with HCO(3)(-) ions for adsorption sites. At a salinity <33, some PO(4)(3-) absorption occurred as CaCO(3) precipitated. Concentrations of PO(4)(3-) between 2 and 5 mu mol/L were released by desorption when the limestone was exposed to seawater. The results of this study suggest that as seawater intrudes into an originally freshwater coastal aquifer, adsorbed PO(4)(3-) may be released into the groundwater. Consequently, adsorbed PO(4)(3-) is expected to be released from coastal carbonate aquifers world-wide as sea level continues to rise exposing more of the freshwater aquifer to seawater. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Price, Rene M.; Jolicoeur, Jean L.] Florida Int Univ, Dept Earth & Environm, Miami, FL 33199 USA. [Price, Rene M.; Jolicoeur, Jean L.] Florida Int Univ, SE Environm Res Ctr, Miami, FL 33199 USA. [Savabi, M. Reza; Roy, Srikumar] ARS, USDA, Miami, FL USA. RP Price, RM (reprint author), Florida Int Univ, Dept Earth & Environm, Miami, FL 33199 USA. EM pricer@fiu.edu FU Southeast Environmental Research Center (SERC) of Florida International University (FIU); National Park Service-Everglades National Park; NSF [DBI-0620409]; USDA, Agricultural Research Service FX This research was supported by grants from the Southeast Environmental Research Center (SERC) of Florida International University (FIU), the National Park Service-Everglades National Park, NSF Grant Number DBI-0620409, and the USDA, Agricultural Research Service. We thank Jim Fourqurean FIU for assistance with the phosphate analysis, and Peter Swart from RSMAS for assistance with the ICP analysis. This is SERC contribution number 479. NR 32 TC 18 Z9 18 U1 8 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0883-2927 J9 APPL GEOCHEM JI Appl. Geochem. PD JUL PY 2010 VL 25 IS 7 BP 1085 EP 1091 DI 10.1016/j.apgeochem.2010.04.013 PG 7 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA 614XU UT WOS:000279093900014 ER PT J AU Ma, MG Liu, ZL AF Ma, Menggen Liu, Z. Lewis TI Mechanisms of ethanol tolerance in Saccharomyces cerevisiae SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Review DE Gene expression; Genomic adaptation; Pathway analysis; Regulatory networks; Stress tolerance ID SHOCK TRANSCRIPTION FACTOR; GENOME-WIDE IDENTIFICATION; RESPONSE ELEMENT STRE; BIOMASS CONVERSION INHIBITORS; FATTY-ACID-COMPOSITION; HEAT-SHOCK; PLASMA-MEMBRANE; PROTEIN-KINASE; INTRACELLULAR ACIDIFICATION; LIPID-COMPOSITION AB Saccharomyces cerevisiae is a superb ethanol producer, yet is also sensitive to higher ethanol concentrations especially under high gravity or very high gravity fermentation conditions. Ethanol tolerance is associated with interplay of complex networks at the genome level. Although significant efforts have been made to study ethanol stress response in past decades, mechanisms of ethanol tolerance are not well known. With developments of genome sequencing and genomic technologies, our understanding of yeast biology has been revolutionarily advanced. More evidence of mechanisms of ethanol tolerance have been discovered involving multiple loci, multi-stress, and complex interactions as well as signal transduction pathways and regulatory networks. Transcription dynamics and profiling studies of key gene sets including heat shock proteins provided insight into tolerance mechanisms. A transient gene expression response or a stress response to ethanol does not necessarily lead to ethanol tolerance in yeast. Reprogrammed pathways and interactions of cofactor regeneration and redox balance observed from studies of tolerant yeast demonstrated the significant importance of a time-course study for ethanol tolerance. In this review, we focus on current advances of our understanding for ethanol-tolerance mechanisms of S. cerevisiae including gene expression responses, pathway-based analysis, signal transduction and regulatory networks. A prototype of global system model for mechanisms of ethanol tolerance is presented. C1 [Ma, Menggen; Liu, Z. Lewis] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Liu, ZL (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM ZLewis.Liu@ars.usda.gov FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2006-35504-17359] FX The authors thank Michael A. Cotta for critically reading the manuscript and helpful suggestions. This study was supported in part by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2006-35504-17359. NR 122 TC 96 Z9 104 U1 5 U2 78 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUL PY 2010 VL 87 IS 3 BP 829 EP 845 DI 10.1007/s00253-010-2594-3 PG 17 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 611JA UT WOS:000278810200004 PM 20464391 ER PT J AU Zhu, JY Pan, XJ Zalesny, RS AF Zhu, J. Y. Pan, Xuejun Zalesny, Ronald S., Jr. TI Pretreatment of woody biomass for biofuel production: energy efficiency, technologies, and recalcitrance SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Review DE Recalcitrance; Cellulosic ethanol; Woody/Forest biomass; SPORL Enzymatic hydrolysis/saccharification; Pretreatment ID ENHANCED ENZYMATIC-HYDROLYSIS; HYBRID POPLAR PLANTATIONS; LODGEPOLE PINE; STEAM PRETREATMENT; ETHANOL-PRODUCTION; LIGNOCELLULOSIC BIOMASS; CELLULOSE HYDROLYSIS; ORGANOSOLV PROCESS; ALCELL(R) LIGNIN; UNITED-STATES AB This mini review discusses several key technical issues associated with cellulosic ethanol production from woody biomass: energy consumption for woody biomass pretreatment, pretreatment energy efficiency, woody biomass pretreatment technologies, and quantification of woody biomass recalcitrance. Both total sugar yield and pretreatment energy efficiency, defined as the total sugar recovery divided by total energy consumption for pretreatment, should be used to evaluate the performance of a pretreatment process. A post-chemical pretreatment wood size-reduction approach was proposed to significantly reduce energy consumption. The review also emphasizes using a low liquid-to-wood ratio (L/W) to reduce thermal energy consumption for any thermochemical/physical pretreatment in addition to reducing pretreatment temperature. C1 [Zhu, J. Y.; Pan, Xuejun] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Zalesny, Ronald S., Jr.] US Forest Serv, USDA, No Res Stn, Rhinelander, WI USA. RP Zhu, JY (reprint author), Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. EM jzhu@fs.fed.us NR 83 TC 113 Z9 115 U1 9 U2 78 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUL PY 2010 VL 87 IS 3 BP 847 EP 857 DI 10.1007/s00253-010-2654-8 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 611JA UT WOS:000278810200005 PM 20473606 ER PT J AU Mullaney, EJ Locovare, H Sethumadhavan, K Boone, S Lei, XG Ullah, AHJ AF Mullaney, Edward J. Locovare, Heather Sethumadhavan, Kandan Boone, Stephanie Lei, Xin Gen Ullah, Abul H. J. TI Site-directed mutagenesis of disulfide bridges in Aspergillus niger NRRL 3135 phytase (PhyA), their expression in Pichia pastoris and catalytic characterization SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Phytase; Histidine acid phosphatase; Disulfide bridge; Aspergillus niger ID PANCREATIC TRYPSIN-INHIBITOR; ESCHERICHIA-COLI PHYTASE; HISTIDINE ACID PHYTASES; CONFORMATIONAL STABILITY; FUMIGATUS PHYTASE; BONDS; FICUUM; THERMOSTABILITY; IMPROVES; MUTANTS AB Earlier studies have established the importance of five disulfide bridges (DBs) in Aspergillus niger phytase. In this study, the relative importance of each of the individual disulfide bridge is determined by its removal by site-directed mutagenesis of specific cysteines in the cloned A. niger phyA gene. Individually, these mutant phytases were expressed in a Pichia expression system and their product purified and characterized. The removal of disulfide bridge 2 yielded a mutant phytase with a complete loss of catalytic activity. The other disulfide mutants displayed a broad array of altered catalytic properties including a lower optimum temperature from 58A degrees C to 53A degrees C for bridge number 1, 37A degrees C for bridge number 3 and 4, and 42A degrees C for bridge number 5. The pH versus activity profile was also modified in the DB mutants. The pH profile of the wild-type phytase was modified by the DB mutations. In bridge number 1, 3, and 4, the second peak at pH 2.5 was abolished, and in bridge number 5, the peak at pH 5.0 was abolished completely leaving only the pH 2.5. While the K (m) was not affected drastically, the turnover number was lowered significantly in bridge number 3, 4, and 5. C1 [Mullaney, Edward J.; Locovare, Heather; Sethumadhavan, Kandan; Boone, Stephanie; Ullah, Abul H. J.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Lei, Xin Gen] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA. RP Mullaney, EJ (reprint author), ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM ed.mullaney@ars.usda.gov; abul.ullah@ars.usda.gov NR 23 TC 9 Z9 10 U1 3 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUL PY 2010 VL 87 IS 4 BP 1367 EP 1372 DI 10.1007/s00253-010-2542-2 PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 616HU UT WOS:000279200600016 PM 20376636 ER PT J AU Qin, SP Hu, CS He, XH Dong, WX Cui, JF Wang, Y AF Qin, Shuping Hu, Chunsheng He, Xinhua Dong, Wenxu Cui, Junfang Wang, Ying TI Soil organic carbon, nutrients and relevant enzyme activities in particle-size fractions under conservational versus traditional agricultural management SO APPLIED SOIL ECOLOGY LA English DT Article DE Conservational agriculture; Particle-size fractions; Soil enzyme activities; Soil nitrogen and phosphorus; Soil organic carbon ID MICROBIAL BIOMASS; STABILIZATION MECHANISMS; AGGREGATE SIZE; TILLAGE; MATTER; NITROGEN; QUANTIFICATION; MICROORGANISMS; SEQUESTRATION; PRODUCTIVITY AB Micro-scale investigation is helpful for better understanding of the relationships between organic matter, microorganisms and nutrients in soil, and for better interpretation of modifications induced by soil management. The soil particle-size fractions (2000-200, 200-63, 63-2, and 2-0.1 mu m), contents of soil organic carbon (SOC), total N (STN), available P (SAP), dissolved organic C (DOC), light fraction organic C (LOC), microbial biomass N (MBN), basal respiration (SBR), and relevant enzyme activities of C, N and P transformations, such as beta-glucosidase (beta-G), N-acetyl-beta-glucosaminidase (N-G), protease, urease and alkaline phosphomonoesterase (APH) were analyzed to study the effects of 8-year-period conservational (no-till with residue retention) (CAM) versus traditional agricultural management (moldboard plowing without residue retention) (TAM) to a Haplic Cambisol soil in the North China Plain (NCP). Our results showed that CAM significantly enlarged the stocks of SOC, DOC, LOC, STN and SAP in the 0-10 cm layer, increased the contents of SOC, STN and SAP in the sand fractions, and promoted all of the enzyme activities in the bulk soil and all of the four particle-size fractions. Our results suggested that CAM increased the nutrient contents in the sand fractions by both enlarging the content of particulate organic matter and enhancing the activities of enzymes involved in nutrient cycling in these fractions. On the contrary, the contents of SOC and nutrients in the silt and clay fractions were relatively resistant to the conversion from CAM to TAM, indicating the limitation of CAM for stable SOC sequestration. (C) 2010 Elsevier B.V. All rights reserved. C1 [Qin, Shuping; Hu, Chunsheng; Dong, Wenxu; Cui, Junfang; Wang, Ying] Chinese Acad Sci, Key Lab Agr Water Resources, Ctr Agr Resources Res, Inst Genet & Dev Biol, Shijiazhuang 050021, Hebei, Peoples R China. [Qin, Shuping; Cui, Junfang; Wang, Ying] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [He, Xinhua] US Forest Serv, No Res Stn, USDA, Houghton, MI 49931 USA. RP Hu, CS (reprint author), Chinese Acad Sci, Key Lab Agr Water Resources, Ctr Agr Resources Res, Inst Genet & Dev Biol, 286 Huaizhong Rd, Shijiazhuang 050021, Hebei, Peoples R China. EM meimei841114@163.com RI Qin, Shuping /B-4451-2010; Hu, Chunsheng /F-2115-2014; He, Xinhua/B-8047-2015 OI He, Xinhua/0000-0002-5570-3454 FU National Natural Science Foundation of China [30870456]; Chinese Academy of Sciences [KSCX2-YW-449, KZCX2-YW-Q02-02] FX This work was supported by the National Natural Science Foundation of China (30870456) and the Innovation Knowledge Project of the Chinese Academy of Sciences (Grant Nos. KSCX2-YW-449 and KZCX2-YW-Q02-02). We are grateful to Xiaoqing Bi for soil sampling. NR 45 TC 32 Z9 38 U1 3 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JUL PY 2010 VL 45 IS 3 BP 152 EP 159 DI 10.1016/j.apsoil.2010.03.007 PG 8 WC Soil Science SC Agriculture GA 633XX UT WOS:000280541700006 ER PT J AU Davis, LK Fox, BK Lim, C Lerner, DT Hirano, T Grau, EG AF Davis, Lori K. Fox, Bradley K. Lim, Chhorn Lerner, Darren T. Hirano, Tetsuya Grau, E. Gordon TI Effects of 11-ketotestosterone and fishmeal in the feed on growth of juvenile tilapia (Oreochromis mossambicus) SO AQUACULTURE LA English DT Article DE Mozambique tilapia; Fishmeal-free diet; 11-ketotestosterone; 17 beta-estradiol; Growth; Vitellogenin ID 17 ALPHA-METHYLTESTOSTERONE; PLANT PROTEIN-SOURCES; FACTOR-I PRODUCTION; NILE TILAPIA; TELEOST FISH; ESTROGENIC ACTIVITY; VITELLOGENIN PRODUCTION; ONCORHYNCHUS-KISUTCH; COMPLETE REPLACEMENT; EURYHALINE TILAPIA AB The effects of 11-ketotestosterone (11KT) and dietary fishmeal on growth of tilapia (Oreochromis mossambicus) were examined. Juvenile tilapia, weighing about 8 g, were reared for 50 days with isonitrogenous and isocaloric diets containing fishmeal or all plant proteins, each with or without supplementation of 11KT. Fish fed fishmeal-based (FM) diets grew significantly faster than those fed soybean meal-based (SM) diets. Addition of 11KT (10 mg/kg) to the FM diet significantly augmented growth. whereas no significant effect of 11KT was observed in fish fed SM diet. In accord with the accelerated growth, specific growth rates of fish fed FM diets were significantly greater than those of fish fed SM diets, although addition of 11KT to FM or SM diet had no effect on this parameter. Males showed significantly higher plasma levels of insulin-like growth factor-I (IGF-I) than did females; however, there was no consistent effect of fishmeal or 11KT on plasma IGF-I levels within each sex. Plasma levels of 11KT were significantly higher in males than in females. In males, plasma 11KT levels were significantly higher in fish fed FM diets than in those fed SM diet. No significant effect of fishmeal or 11KT was observed on plasma levels of 11KT or 17 beta-estradiol (E(2)) in females. Low but significant levels of vitellogenin (Vg) were found in male plasma. Plasma Vg levels were significantly lower in fish fed SM diets than in fish fed FM diets in both males and females. These results indicate that addition of 11KT to the fishmeal-based diet stimulated growth, minimally affecting plasma levels of IGF-I, 11KT, E(2) and Vg. The absence of effect of 11KT in fish fed the soybean meal-based diet may indicate that the estrogenic activities or some components in the SM diet are likely to interfere with the growth-promoting effects of 11KT. (C) 2010 Elsevier B.V. All rights reserved. C1 [Davis, Lori K.; Fox, Bradley K.; Lerner, Darren T.; Hirano, Tetsuya; Grau, E. Gordon] Univ Hawaii Manoa, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA. [Lerner, Darren T.] Univ Hawaii Manoa, Univ Hawaii Sea Grant Coll Program, Honolulu, HI 96822 USA. [Lim, Chhorn] ARS, Aquat Anim Hlth Res Lab, USDA, Auburn, AL 36832 USA. RP Grau, EG (reprint author), Univ Hawaii Manoa, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA. EM grau@hawaii.edu FU National Science Foundation [IOB 05-17769]; National Research Initiative, USDA National Institute of Food and Agriculture [2005-35206-15285, 2008-35206-18785, 2008-35206-18787]; Pauley Foundation FX This work was supported in part by grants from National Science Foundation (IOB 05-17769), National Research Initiative Competitive Grant no. 2005-35206-15285, 2008-35206-18785 and 2008-35206-18787 from the USDA National Institute of Food and Agriculture, and the Pauley Foundation Summer Program 2008. We are grateful to Ms. Elizabeth Cerny-Chipman and Ms. Sonia Fang, Pomona College, for their technical assistance. NR 46 TC 12 Z9 15 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD JUL 1 PY 2010 VL 305 IS 1-4 BP 143 EP 149 DI 10.1016/j.aquaculture.2010.04.006 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 622EN UT WOS:000279643800021 ER PT J AU Barta, C Dunkle, AM Wachter, RM Salvucci, ME AF Barta, Csengele Dunkle, Alison M. Wachter, Rebekka M. Salvucci, Michael E. TI Structural changes associated with the acute thermal instability of Rubisco activase SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE Photosynthetic CO2 fixation; Heat stress; Thermal stability; Calvin cycle; Adenine nucleotides; Magnesium ions ID PROTEIN SECONDARY STRUCTURE; CIRCULAR-DICHROISM SPECTRA; MODERATE HEAT-STRESS; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE; POSSIBLE MECHANISM; HIGH-TEMPERATURES; LEAF TEMPERATURE; PHOTOSYNTHESIS; ARABIDOPSIS; LEAVES AB Inhibition of photosynthesis by heat has been linked to the instability of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) chaperone, Rubisco activase. Examination of the recombinant enzyme showed that ADP and ATP protected against inactivation, whereas Mg2+ promoted inactivation. Heating caused aggregation of Rubisco activase characterized by disruption of secondary structure content and formation of insoluble protein. In contrast, incubation at room temperature without nucleotide caused the active similar to 660 kDa protein to form a soluble, but inactive aggregate of >2 x 10(6) Da. Circular dichroism (CD) spectroscopy and fluorescence established that structural perturbations in the aggregate did not reduce alpha-helical content significantly. Differences in the thermal stability between wild type and mutant Rubisco activase were observed for the recombinant proteins and when the proteins were expressed in transgenic Arabidopsis. That the sensitivity of these plants to heat differs indicates that the thermal instability of Rubisco activase is a main determinant of the temperature-sensitivity of photosynthesis. Published by Elsevier Inc. C1 [Barta, Csengele; Salvucci, Michael E.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85139 USA. [Dunkle, Alison M.; Wachter, Rebekka M.] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA. RP Salvucci, ME (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85139 USA. EM csengele.barta@ars.usda.gov; achausse@asu.edu; rwachter@asu.edu; mike.salvucci@ars.usda.gov FU United States Department of Energy, Office of Basic Energy Sciences [DE-FG02-08ER20268, DE-PS02-09ER09-02, DE-FG02-09ER16123] FX We thank Nancy Parks for her expert technical assistance (Agricultural Research Service, USDA) and the Arizona State University (ASU) for the use of facilities. Research was supported by the United States Department of Energy, Office of Basic Energy Sciences, Photosynthetic Systems Grants DE-FG02-08ER20268 and DE-PS02-09ER09-02 (M.E.S.) and DE-FG02-09ER16123 (R.M.W.). NR 43 TC 21 Z9 22 U1 1 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 EI 1096-0384 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD JUL PY 2010 VL 499 IS 1-2 BP 17 EP 25 DI 10.1016/j.abb.2010.04.022 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 613CE UT WOS:000278952900003 PM 20450882 ER PT J AU Olafson, PU Lohmeyer, KH Dowd, SE AF Olafson, Pia Untalan Lohmeyer, Kimberly H. Dowd, Scot E. TI ANALYSIS OF EXPRESSED SEQUENCE TAGS FROM A SIGNIFICANT LIVESTOCK PEST, THE STABLE FLY (Stomoxys calcitrans), IDENTIFIES TRANSCRIPTS WITH A PUTATIVE ROLE IN CHEMOSENSATION AND SEX DETERMINATION SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Stomoxys calcitrans; stable fly; pyrosequencing; chemosensory; sex determination; reproductive behavior ID ODORANT-BINDING PROTEINS; PHEROMONE-SENSITIVE NEURONS; MEDITERRANEAN FRUIT-FLY; DROSOPHILA-MELANOGASTER; CERATITIS-CAPITATA; FLIES DIPTERA; GENE FAMILY; TRANSFORMER GENE; MEGASELIA-SCALARIS; COURTSHIP BEHAVIOR AB The stable fly, Stomoxys calcitrans L. (Diptera: Muscidae), is one of the most significant pests of livestock in the United States. The identification of targets for the development of novel control for this pest species, focusing on those molecules that play a role in successful feeding and reproduction, is critical to mitigating its impact on confined and rangeland livestock. A database was developed representing genes expressed at the immature and adult life stages of the stable fly, comprising data obtained from pyrosequencing both immature and adult stages and from small-scale sequencing of an antennal/maxillary palp-expressed sequence tag library. The full-length sequence and expression of 21 transcripts that may have a role in chemosensation is presented, including 13 odorant-binding proteins, 6 chemosensory proteins, and 2 odorant receptors. Transcripts with potential roles in sex determination and reproductive behaviors are identified, including evidence for the sex-specific expression of stable fly doublesex- and transformer-like transcripts. The current database will be a valuable tool for target identification and for comparative studies with other Diptera. Published 2010 Wiley Periodicals, Inc. C1 [Olafson, Pia Untalan; Lohmeyer, Kimberly H.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Dowd, Scot E.] Res & Testing Lab, Lubbock, TX USA. RP Olafson, PU (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM pia.olafson@ars.usda.gov NR 81 TC 11 Z9 11 U1 3 U2 6 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0739-4462 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD JUL PY 2010 VL 74 IS 3 BP 179 EP 204 PG 26 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 644SI UT WOS:000281399400004 PM 20572127 ER PT J AU Noel, SE Lai, CQ Mattei, J Parnell, LD Ordovas, JM Tucker, KL AF Noel, Sabrina E. Lai, Chao-Qiang Mattei, Josiemer Parnell, Laurence D. Ordovas, Jose M. Tucker, Katherine L. TI Variants of the CD36 gene and metabolic syndrome in Boston Puerto Rican adults SO ATHEROSCLEROSIS LA English DT Article DE Puerto Rican; Hispanic; CD36; Metabolic syndrome ID HIGH-DENSITY-LIPOPROTEIN; INSULIN-RESISTANCE; FATTY-ACID; CARDIOVASCULAR-DISEASE; DIABETES-MELLITUS; HYPERTENSIVE-RATS; PHYSICAL-ACTIVITY; SKELETAL-MUSCLE; HISPANIC ELDERS; POPULATION AB Objective: Puerto Ricans experience a high prevalence of several chronic conditions, including metabolic syndrome. Genetic variants of the CD36 gene have been associated with metabolic syndrome. We aimed to determine the association between 6 single nucleotide polymorphisms (SNPs) for CD36 and metabolic syndrome and its components in Puerto Ricans (45-75 year) living in the Greater Boston area. Methods: Associations between each SNP, metabolic syndrome and its components were examined using multivariate logistic regression models. Haplotype trend regression analysis was used to determine associations between haplotypes and metabolic syndrome. Results: For two SNPs of CD36 (rs1049673 and rs3211931), homozygous subjects of the minor allele (G and T, respectively) were associated with a higher likelihood of metabolic syndrome (odds ratio (OR) (95% confidence interval (CI)): 1.89 (1.0, 3.5) and 1.77 (1.0, 3.1), respectively) relative to carriers of the major allele. Although CD36 haplotypes were not significantly associated with metabolic syndrome overall (global significance, P = 0.23), one haplotype (G-C-C vs. C-C-C (reference haplotype)) was marginally associated (P = 0.049). Conclusion: SNPs of CD36 were associated with metabolic syndrome in Puerto Ricans. Prospective studies should further explore the role of CD36 variants in the development of this condition. (C) 2010 Elsevier Ireland Ltd. All rights reserved. C1 [Noel, Sabrina E.; Lai, Chao-Qiang; Mattei, Josiemer; Parnell, Laurence D.; Ordovas, Jose M.; Tucker, Katherine L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Tucker, KL (reprint author), Northeastern Univ, Dept Hlth Sci, 316 Robinson Hall, Boston, MA 02115 USA. EM kl.tucker@neu.edu RI Tucker, Katherine/A-4545-2010; Mattei, Josiemer/H-1800-2016; OI Mattei, Josiemer/0000-0001-5424-8245; Tucker, Katherine/0000-0001-7640-662X; Ordovas, Jose/0000-0002-7581-5680 FU NIH [5P01-AG023394]; United States Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This study was supported by NIH 5P01-AG023394 and by the United States Department of Agriculture, Agricultural Research Service agreement number 58-1950-7-707. NR 32 TC 23 Z9 23 U1 0 U2 2 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 J9 ATHEROSCLEROSIS JI Atherosclerosis PD JUL PY 2010 VL 211 IS 1 BP 210 EP 215 DI 10.1016/j.atherosclerosis.2010.02.009 PG 6 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 620VV UT WOS:000279530000039 PM 20223461 ER PT J AU Dayan, FE Daga, PR Duke, SO Lee, RM Tranel, PJ Doerksen, RJ AF Dayan, Franck E. Daga, Pankaj R. Duke, Stephen O. Lee, Ryan M. Tranel, Patrick J. Doerksen, Robert J. TI Biochemical and structural consequences of a glycine deletion in the alpha-8 helix of protoporphyrinogen oxidase SO BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS LA English DT Article DE Codon deletion; Mechanism of resistance; Evolution of resistance; Helix capping; Herbicide resistance; Binding kinetics; Molecular dynamics simulation ID WATERHEMP AMARANTHUS-RUDIS; DIPHENYL ETHER HERBICIDES; IX OXIDASE; INHIBITING HERBICIDES; PHOTOBLEACHING HERBICIDES; PEROXIDIZING HERBICIDES; OXIDIZING ACTIVITIES; PHYSIOLOGICAL-BASIS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI AB A rare Gly210 deletion in protoporphyrinogen oxidase (PPO) was recently discovered in herbicide-resistant Amaranthus tuberculatus. According to the published X-ray structure of Nicotiana tabacum PPO, Gly210 is adjacent to, not in, the PPO active site, so it is a matter of interest to determine why its deletion imparts resistance to herbicides. In our kinetic experiments, this deletion did not affect the affinity of protoporphyrinogen IX nor the FAD content, but decreased the catalytic efficiency of the enzyme. The suboptimal K(cat) was compensated by a significant increase in the K(i)S for inhibitors and a switch in their interactions from competitive to mixed-type inhibition. In our protein modeling studies on herbicide-susceptible PPO and resistant PPO, we show that Gly210 plays a key role in the alpha L helix-capping motif at the C-terminus of the alpha-8 helix which helps to stabilize the helix. In molecular dynamics simulations, the deletion had significant architecture consequences, destabilizing the alpha-8 helix-capping region and unraveling the last turn of the helix, leading to enlargement of the active site cavity by similar to 50%. This seemingly innocuous deletion of Gly210 of the mitochondrial PPO imparts herbicide resistance to this dual-targeted protein without severely affecting its normal physiological function, which may explain why this unusual mutation was the favored evolutionary path for achieving resistance to PPO inhibitors. Published by Elsevier B.V. C1 [Dayan, Franck E.; Duke, Stephen O.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Daga, Pankaj R.; Doerksen, Robert J.] Univ Mississippi, Sch Pharm, Dept Med Chem, University, MS 38677 USA. [Lee, Ryan M.; Tranel, Patrick J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Doerksen, Robert J.] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, University, MS 38677 USA. RP Dayan, FE (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, POB 8048, University, MS 38677 USA. EM fdayan@olemiss.edu RI Daga, Pankaj/D-6047-2013; Dayan, Franck/A-7592-2009; OI Dayan, Franck/0000-0001-6964-2499; Daga, Pankaj/0000-0002-2508-0903 FU National Science Foundation [EPS-0556308]; NIH National Center for Research Resources [C06 RR-14503-01] FX We thank Susan Watson for her excellent technical assistance. Thanks to Dr. Ronak Y. Patel for helpful discussions. MCSR computing facilities are greatly appreciated. This research was funded in part by National Science Foundation EPS-0556308 and was conducted in part in a facility constructed with support from research facilities improvement program C06 RR-14503-01 from the NIH National Center for Research Resources. NR 57 TC 18 Z9 18 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-9639 J9 BBA-PROTEINS PROTEOM JI BBA-Proteins Proteomics PD JUL PY 2010 VL 1804 IS 7 BP 1548 EP 1556 DI 10.1016/j.bbapap.2010.04.004 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 609OZ UT WOS:000278667700018 PM 20399914 ER PT J AU Mueller-Warrant, GW Banowetz, GM Whittaker, GW AF Mueller-Warrant, George W. Banowetz, Gary M. Whittaker, Gerald W. TI Geospatial identification of optimal straw-to-energy conversion sites in the Pacific Northwest SO BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR LA English DT Article DE straw; biomass; syngas; pyrolysis bio-oil; facility siting: K-means, spatial clustering; transportation cost; infrastructure components ID BIOFUELS; GASIFICATION; EMISSIONS; BIOMASS AB Previous attempts to develop straw-based bioenergy systems have stumbled at costs of transporting this low-density resource to large-scale, centralized facilities. Success in developing small-scale, distributed technologies (e.g syngas or pyrolysis bio-oil) that reduce these costs will depend on closely matching system requirements to spatial distribution of available straw. We analyzed straw distribution in the Pacific Northwest to identify optimal sites for facilities ranging from a pilot plant currently under development to larger ones of previous studies. Sites for plants with capacities of 1, 10, or 100 million kg straw y(-1) were identified using a 'lowest-hanging-fruit' iterative siting process in which the location of maximum density of straw over an appropriately sized neighborhood was identified, distance from that point necessary to include desired quantity of straw measured, straw assigned to that plant removed from the raster, and the process repeated until all available straw had been assigned Compared to K-means, our new method sited the first 44% of plants at superior locations in terms of local straw density (i.e. lower transportation costs) and the next 39% at equivalent locations. K-means produced better locations for the final 17% of plants along with superior average results. For the smallest facilities at locations defined by 3-year average available straw density, 1.2 km buffers were adequate to provide straw for the first 10% of plants, with twice that distance sufficient for the first 70%. For the largest plants, 12 km buffers satisfied the first 10% of plants, with 24 km buffers satisfying the first 60%. Buffer distances exceeded 68 km for the final 20% of the largest plants. Siting patterns for the smallest plants were more evenly distributed than larger ones, suggesting that farm-scale technology may be more politically appealing. Smaller plants, however, suffered from higher year-to-year variability in straw supply within pre-defined distances. Published in 2010 by John Wiley & Sons, Ltd. C1 [Mueller-Warrant, George W.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Mueller-Warrant, GW (reprint author), ARS, USDA, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. NR 24 TC 5 Z9 5 U1 0 U2 5 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 1932-104X J9 BIOFUEL BIOPROD BIOR JI Biofuels Bioprod. Biorefining PD JUL-AUG PY 2010 VL 4 IS 4 BP 385 EP 407 DI 10.1002/bbb.230 PG 23 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA 633DO UT WOS:000280479900013 ER PT J AU Green, MB Finlay, JC AF Green, Mark B. Finlay, Jacques C. TI Patterns of hydrologic control over stream water total nitrogen to total phosphorus ratios SO BIOGEOCHEMISTRY LA English DT Article DE Catchment hydrology; Connectivity; N:P ratios; Stoichiometry; Stream ecology ID NUTRIENT LIMITATION; ORGANIC-CARBON; TEMPORAL VARIABILITY; SEASONAL PATTERNS; SURFACE-WATER; LAKE-SUPERIOR; LAND-USE; N-P; TRANSPORT; RIVERS AB Many ecologists and biogeochemists explore the interaction of the nitrogen (N) and phosphorus (P) cycles by addressing N:P ratios. While N:P ratios are recognized as broadly important to the composition and functioning of lotic ecosystems, the fundamental controls on stream water N:P ratio variation remains poorly understood. Low N:P ratio (less than 16) streams appear more likely in arid climates than in mesic climates, suggesting possible hydrologic or landscape controls. We explored the importance of watershed hydrology to the variation of total N to total P (TN:TP) ratios in stream water, and whether such variation is characteristically different across watershed classes based on mean annual precipitation and median observed TN:TP ratio. Nonparametric scatter plot analysis was applied to normalized TN:TP ratios and associated discharge (Q) measurements from 57 minimally-impacted watersheds from the contiguous United States. At the seasonal scale, TN:TP ratios showed a negative relationship with Q in semiarid climates and a positive relationship with Q in humid climates. Over storm event scales, TN:TP ratios decline with increasing Q across all watershed classes. The results broadly indicate hydrology is an important driver of TN:TP ratio variation over multiple time scales. We hypothesize that the broad differences across watershed classes are driven by variation in the nature of connectivity (frequency and magnitude of connections) of the landscape to streams. A strong physical control of N:P ratios in stream water is in stark contrast to the biological control of N:P ratios in the oceans, suggesting that application of stoichiometric theory-developed using marine systems-to lotic systems requires a broader consideration of controlling factors. C1 [Green, Mark B.] Univ Minnesota, Grad Program Water Resources Sci, St Paul, MN 55108 USA. [Green, Mark B.; Finlay, Jacques C.] Natl Ctr Earth Surface Dynam, Minneapolis, MN USA. [Green, Mark B.] US Forest Serv, No Res Stn, Durham, NH USA. [Finlay, Jacques C.] Univ Minnesota, Dept Ecol Evolut & Behav, Minneapolis, MN 55455 USA. RP Green, MB (reprint author), Plymouth State Univ, Ctr Environm, Plymouth, NH USA. EM mbgreen@plymouth.edu RI Finlay, Jacques/B-6081-2011; OI Finlay, Jacques/0000-0002-7968-7030; Green, Mark/0000-0002-7415-7209 FU National Science Foundation via the National Center for Earth-Surface Dynamics [EAR-0120914]; USDA FX We thank two anonymous reviewers whose insights improved the communication of this work. This work was supported by the STC program of the National Science Foundation via the National Center for Earth-Surface Dynamics under Agreement EAR-0120914. Also, M.G. was supported via a USDA National Needs Fellowship. NR 52 TC 18 Z9 20 U1 3 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 J9 BIOGEOCHEMISTRY JI Biogeochemistry PD JUL PY 2010 VL 99 IS 1-3 BP 15 EP 30 DI 10.1007/s10533-009-9394-9 PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 613ZV UT WOS:000279025100002 ER PT J AU Shapiro-Ilan, DI Cottrell, TE Mizell, RF Horton, DL Behle, RW Dunlap, CA AF Shapiro-Ilan, David I. Cottrell, Ted E. Mizell, Russell F., III Horton, Dan L. Behle, Robert W. Dunlap, Christopher A. TI Efficacy of Steinernema carpocapsae for control of the lesser peachtree borer, Synanthedon pictipes: Improved aboveground suppression with a novel gel application SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Entomopathogenic nematode; Formulation; Peach; Steinernema carpocapsae; Synanthedon pictipes ID ENTOMOPATHOGENIC NEMATODES; PLUTELLA-XYLOSTELLA; BIOLOGICAL-CONTROL; LEPIDOPTERA; SESIIDAE; PESTS; SUSCEPTIBILITY; ALGINATE; MOTH AB Safe and effective tactics are needed for control of the lesser peachtree borer, Synanthedon pictipes (Grote & Robinson), which is a major pest of stone fruits (Prunus spp) in eastern North America. Virulence of the entomopathogenic nematode, Steinernema carpocapsae (Weiser), to S. pictipes has been demonstrated in the laboratory. However, achieving field efficacy has been difficult because S. pictipes attacks the tree aboveground where nematodes are subjected to damaging environmental conditions, e.g., UV radiation and desiccation. We investigated the potential of various formulations to improve the efficacy of aboveground applications. First, we screened five potential adjuvants at 2%, 20%, and 40% concentrations in water for toxicity to S. carpocapsae in the laboratory: Anti-Stress, Moisturin (R), Nu-Film (R), Shatter-Proof, and Transfilm (R). In general, the adjuvants did not adversely affect nematode survival except at the highest rate. Subsequently, Shatter-Proof was tested in field trials in 2008 and 2009. S. carpocapsae was applied alone or with Shatter-Proof to peach limbs pre-infested with S. pictipes larvae. Furthermore, the experiments included the following treatments: S. carpocapsae followed by a post-application covering of latex paint, moistened diaper, or a gel spray (Barricade (R)). Controls of water-only, or water plus Shatter-Proof, Barricade (R), or paint (without nematodes) were also included. The nematodes-only treatment failed to reduce S. pictipes survival relative to the water-only control in either year. Additionally, the nematode treatments with Shatter-Proof or paint did not differ from nematodes-only, water-only or their respective control treatments without nematodes. The diaper treatment with nematodes showed some potential as an efficacy enhancer (e.g., insect survival was reduced relative to nematodes-only in one year). In contrast, in both years, nematodes plus Barricade (R) reduced S. pictipes relative to the controls and the nematodes-only treatment; survival in the Barricade (R) treatment was 30% and 0% in 2008 and 2009, respectively. We conclude that nematode treatments followed by application of a sprayable gel such as Barricade (R) can enhance control of S. pictipes and possibly other aboveground pests as well. Published by Elsevier Inc. C1 [Shapiro-Ilan, David I.; Cottrell, Ted E.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Mizell, Russell F., III] Univ Florida, Dept Entomol, Quincy, FL USA. [Horton, Dan L.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Behle, Robert W.; Dunlap, Christopher A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM David.Shapiro@ars.usda.gov FU USDA-CSREES [34381-16937] FX The authors thank A. Amis, R. Auman, H. Bartels, T. Brearley, W. Evans, K. Halat, B. Hamilton, B. Hartley, C. Paulsen, S. Starks, M. Thomas, and C. Vandergrift for providing technical assistance. This study was supported in part by USDA-CSREES PMAP (Pest Management Alternative Program) grant No. 34381-16937. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the United States Department of Agriculture. NR 36 TC 21 Z9 23 U1 0 U2 17 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2010 VL 54 IS 1 BP 23 EP 28 DI 10.1016/j.biocontrol.2009.11.009 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 591ZT UT WOS:000277344800005 ER PT J AU Castrillo, LA Griggs, MH Liu, HP Bauer, LS Vandenberg, JD AF Castrillo, Louela A. Griggs, Michael H. Liu, Houping Bauer, Leah S. Vandenberg, John D. TI Assessing deposition and persistence of Beauveria bassiana GHA (Ascomycota: Hypocreales) applied for control of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), in a commercial tree nursery SO BIOLOGICAL CONTROL LA English DT Article DE Fraxinus spp.; Invasive insect; Entomopathogenic fungus; Microbial control; Real-time PCR ID METARHIZIUM-ANISOPLIAE; CONIDIA; GREENHOUSE; EXPOSURE; ALFALFA; DAMAGE AB Determining the deposition and field persistence of mycoinsecticides is essential in the development of effective and economical application strategies, including specifically the timing and frequency of spray applications. In this study we used three methods to evaluate the persistence of Beauveria bassiana strain GHA applied for control of the emerald ash borer (EAB), Agrilus planipennis, an invasive pest attacking ash trees, Fraxinus spp., in North America. Fungal inocula present on ash bark and leaves, collected at 61, 7 and 14 days after spraying, were quantified by use of molecular (real-time PCR assay) and culture-based methods (semi-selective wheat germ dodine agar). We also assayed fungus-sprayed leaves and bark against adult beetles to determine whether the level of inocula persisting in the field was sufficient to affect beetle survival. Our data quantified deposition of B. bassiana and documented fungal persistence. We observed significant decline in recovery of colony-forming units of B. bassiana within 1 week of application. The decline was more pronounced on leaves than on bark, and was also evident in loss of virulence of treated substrates to adult EAB with increasing time after application. However, sufficient inocula persisted on bark and leaves for 7-14 days to cause 40-57% and 17-38% mortality, respectively, making pre-emergent sprays a practical means to target adults during emergence, pre-oviposition feeding, or oviposition. (C) 2010 Elsevier Inc. All rights reserved. C1 [Castrillo, Louela A.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Griggs, Michael H.; Vandenberg, John D.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Liu, Houping; Bauer, Leah S.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Bauer, Leah S.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. RP Castrillo, LA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM lac48@cornell.edu NR 20 TC 8 Z9 11 U1 0 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2010 VL 54 IS 1 BP 61 EP 67 DI 10.1016/j.biocontrol.2010.03.005 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 591ZT UT WOS:000277344800011 ER PT J AU Huebner, CD AF Huebner, Cynthia D. TI Establishment of an invasive grass in closed-canopy deciduous forests across local and regional environmental gradients SO BIOLOGICAL INVASIONS LA English DT Article DE Environmental gradient; Forest interiors; Invasive plants; Microstegium vimineum ID MICROSTEGIUM-VIMINEUM POACEAE; CENTRAL HARDWOOD FOREST; TRIN. A. CAMUS; PLANT INVASIONS; C-4 GRASS; UNDERSTORY PLANTS; SHADE-TOLERANT; INVASIBILITY; DIVERSITY; PATTERNS AB Establishment of Microstegium vimineum, an invasive exotic grass, in closed-canopy U.S. eastern forests was evaluated across a local (roadside to forest interior) and regional (across two geographic provinces) environmental gradient in West Virginia. The two geographic provinces were the Allegheny Plateau (more mesic) and the Ridge and Valley Province (more xeric). Biotic, abiotic, and disturbance variables were measured in (1) systematically located plots, starting from the roadside and extending 50 m into the forests, and (2) randomly selected, forest interior plots, with equal numbers of plots containing or not containing M. vimineum. Associations between the variables and the presence of M. vimineum at both scales were evaluated using generalized linear models. Relative importance of the variables related to M. vimineum establishment in the forest interior plots at the regional scale was determined using logistic regression. Results confirmed Microstegium vimineum's reduced reproductive capacity in the forest interior compared to the roadside. Patches of M. vimineum in the forest interiors across the regional gradient were best defined by high native plant richness and diversity. Greater canopy opening, more moss, and shallower litter depths were also positively and significantly associated with M. vimineum presence, but only during the driest sample year. C1 USDA Forest Serv, No Res Stn, Morgantown, WV 26505 USA. RP Huebner, CD (reprint author), USDA Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. EM chuebner@fs.fed.us RI Liu, Yi-Chun/H-5463-2012 FU USDA Forest Service FX The USDA Forest Service funded this research. I thank H. Smith, M. McKissik, W. Laubscher, G. Short, J. Quinn, and L. Strickler for their help in data collection. We also thank P. Tobin, J. Stanovick, D. Simberloff, and two anonymous reviewers for their editorial comments. NR 56 TC 18 Z9 18 U1 4 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2010 VL 12 IS 7 BP 2069 EP 2080 DI 10.1007/s10530-009-9609-y PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 612KS UT WOS:000278897800015 ER PT J AU Huebner, CD AF Huebner, Cynthia D. TI Spread of an invasive grass in closed-canopy deciduous forests across local and regional environmental gradients SO BIOLOGICAL INVASIONS LA English DT Article DE Environmental gradient; Forest interiors; Invasive plants; Microstegium vimineum; Seed dispersal; Spread ID MICROSTEGIUM-VIMINEUM; BIOLOGICAL INVASIONS; PLANT INVASIONS; C-4 GRASS; INVADING ORGANISMS; SHADE-TOLERANT; DISPERSAL; DYNAMICS; MODEL; STOCHASTICITY AB Spread of Microstegium vimineum, an invasive exotic grass, in closed-canopy forests of West Virginia, U.S. was evaluated across a local (roadside to forest interior) and regional (across two geographic provinces) environmental gradient. Seed dispersal distances from roadside populations into forest interiors based on seed rain and soil seed bank data were determined. Colonization and extinction of M. vimineum patches were followed over three years (2005-2007), and spread rate was estimated using a reaction-diffusion model. Direct seed dispersal from the roadside populations occurred primarily adjacent to the maternal plants, indicating that the disjunct M. vimineum patches within the forest interiors occurred via secondary seed dispersal over longer distances. Patches of M. vimineum were found as far as 45 m into the forest interior, but M. vimineum seed in the seed bank were only found within 10 m of the roadside population. Colonization rates of the forest interiors were significantly higher for the more mesic sites than the more xeric sites. The same trend was noted for the spread rate. Radial spread rates ranged between 0.16 and 0.50 m year(-1) and forest interiors were estimated to become saturated with M. vimineum in anywhere between 10 (more mesic sites) and 59 (more xeric sites) years. These results support the possibility of accelerating spread rates in forest interiors caused by more long-distance dispersal events, but slowed by a reduction in M. vimineum fitness in shaded or relatively dry environments. C1 USDA Forest Serv, No Res Stn, Morgantown, WV 26505 USA. RP Huebner, CD (reprint author), USDA Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. EM chuebner@fs.fed.us FU USDA Forest Service FX The USDA Forest Service funded this research. Sincere thanks go to H. Smith, M. McKissik, W. Laubscher, G. Short, J. Quinn, and L. Strickler for their help in data collection. We also thank P. Tobin, J. Stanovick, D. Simberloff, and two anonymous reviewers for their editorial comments. NR 48 TC 13 Z9 13 U1 1 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2010 VL 12 IS 7 BP 2081 EP 2089 DI 10.1007/s10530-009-9610-5 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 612KS UT WOS:000278897800016 ER PT J AU Tallamy, DW Ballard, M D'Amico, V AF Tallamy, Douglas W. Ballard, Meg D'Amico, Vincent TI Can alien plants support generalist insect herbivores? SO BIOLOGICAL INVASIONS LA English DT Article DE Alien plants; Invasive species; Biotic resistance; Enemy release; Generalist; Specialist; Insect herbivores ID ENEMY RELEASE HYPOTHESIS; FOOD; INVASIONS; SPECIALIZATION; ASSOCIATIONS; SENSITIVITY; SUCCESS; FOREST; NUMBER; LARVAE AB Simple rearing experiments were conducted to address two questions relevant to understanding how generalist lepidopteran herbivores interact with alien plants. Yellow-striped armyworm (Spodoptera ornithogalli), luna moth (Actias luna), bagworm (Thyridopteryx ephemeraeformis) and white-marked tussock moth (Orgyia leucostigma) were reared from egg to 5th instar on excised foliage in the laboratory to determine the degree to which highly polyphagous lepidopteran herbivores are physiologically capable of surviving and developing on the suite of alien plants naturalized in the mid-Atlantic. Actias luna larvae from a single population were similarly reared on a representative of each of the 25 native plant genera recorded as hosts for this species to compare the diet breadth of a local population with that listed over the entire geographic range of the species. With few exceptions, all four generalists either quickly starved or grew at an unsustainably low rate on alien foliage. Actias luna larvae survived for 18 days on only 44% of the native plants recorded as hosts over the entire range of this insect and thrived on only 7%. The data suggest that (1) alien plants are unlikely to produce as much generalist insect biomass as the native plants they replace and (2) Lepidoptera that qualify as generalists when host breadth is considered over their entire geographic range may express a far more specialized diet within local populations. Both of these conclusions support the hypothesis that alien plant invasions may seriously disrupt terrestrial food webs by reducing the insect biomass required by insectivores in higher trophic levels. C1 [Tallamy, Douglas W.; Ballard, Meg] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. [D'Amico, Vincent] Univ Delaware, US Forest Serv, No Res Stn, Dept Entomol & Wildlife Ecol, Newark, DE USA. RP Tallamy, DW (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, 250 Townsend Hall,531 S Coll Ave, Newark, DE 19716 USA. EM dtallamy@udel.edu NR 40 TC 33 Z9 34 U1 7 U2 62 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2010 VL 12 IS 7 BP 2285 EP 2292 DI 10.1007/s10530-009-9639-5 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 612KS UT WOS:000278897800032 ER PT J AU Han, HS Halbrook, J Pan, F Salazar, L AF Han, Han-Sup Halbrook, Jeff Pan, Fei Salazar, Lucy TI Economic evaluation of a roll-off trucking system removing forest biomass resulting from shaded fuelbreak treatments SO BIOMASS & BIOENERGY LA English DT Article DE Harvesting and transportation; Slash removal; Wildland fires; Hog fuel grinding AB Shaded fuelbreak treatments involve removal of understory brush and small-diameter trees to reduce fire hazards by disconnecting the continuity of fuels. As a result of these treatments, woody biomass (referred to as slash) is piled throughout the treated stand and later burned. Mechanical removal of slash has not been successfully implemented in many areas due to limited accessibility to sites and the high costs associated with collection and transportation of slash. To address these issues, a roll-off truck paired with a small skid-steer loader was used to collect and transport slash to a centralized processing site where slash was ground as hog fuel for energy production. "Roll-off truck" refers to a straight frame truck configuration in which a 30.6-m(3) container is rolled onto and off the straight frame truck by means of a truck-mounted winch system. This study was designed to quantify the operational performance and costs of removing slash piles using a roll-off trucking system in mountainous conditions in northern California. The overall cost to collect and haul hand-piled slash was $26.81/tonne with 22% average moisture content or $34.37/bone dry metric ton. The roll-off trucking system should be used primarily for short hauling distances since trucking costs significantly increase with small increases in hauling distance due to slow traveling speeds and low slash weight being hauled. Financial analysis indicated that contractors can receive high rates of return on their invested capital after accounting for inflation and income taxes, but limited work opportunities are a concern for them. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Han, Han-Sup; Pan, Fei] Humboldt State Univ, Dept Forestry & Wildland Resources, Arcata, CA 95521 USA. [Halbrook, Jeff] Klamath Natl Forest, Ft Jones, CA USA. [Salazar, Lucy] US Forest Serv, USDA, Eureka, CA USA. RP Han, HS (reprint author), Humboldt State Univ, Dept Forestry & Wildland Resources, 1 Harpst St, Arcata, CA 95521 USA. EM hh30@humboldt.edu FU Six Rivers National Forest and State and Private Forestry, Forest Service, US Department of Agriculture. FX This study was funded by the Six Rivers National Forest and State and Private Forestry, Forest Service, US Department of Agriculture. NR 8 TC 9 Z9 9 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD JUL PY 2010 VL 34 IS 7 BP 1006 EP 1016 DI 10.1016/j.biombioe.2010.02.009 PG 11 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 614NS UT WOS:000279067000011 ER PT J AU Li, CL Knierim, B Manisseri, C Arora, R Scheller, HV Auer, M Vogel, KP Simmons, BA Singh, S AF Li, Chenlin Knierim, Bernhard Manisseri, Chithra Arora, Rohit Scheller, Henrik V. Auer, Manfred Vogel, Kenneth P. Simmons, Blake A. Singh, Seema TI Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification SO BIORESOURCE TECHNOLOGY LA English DT Article DE Pretreatment; Ionic liquid; Dilute acid; Comparison; Enzymatic saccharification ID CORN STOVER; LIME PRETREATMENT; CELLULOSE; HYDROLYSIS; LIGNIN; SUGAR; LIGNOCELLULOSE; TECHNOLOGIES; ENHANCEMENT; DEGRADATION AB The efficiency of two biomass pretreatment technologies, dilute acid hydrolysis and dissolution in an ionic liquid, are compared in terms of delignification, saccharification efficiency and saccharide yields with switchgrass serving as a model bioenergy crop. When subject to ionic liquid pretreatment (dissolution and precipitation of cellulose by anti-solvent) switchgrass exhibited reduced cellulose crystallinity, increased surface area, and decreased lignin content compared to dilute acid pretreatment. Pretreated material was characterized by powder X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy and chemistry methods. Ionic liquid pretreatment enabled a significant enhancement in the rate of enzyme hydrolysis of the cellulose component of switchgrass, with a rate increase of 16.7-fold, and a glucan yield of 96.0% obtained in 24 h. These results indicate that ionic liquid pretreatment may offer unique advantages when compared to the dilute acid pretreatment process for switchgrass. However, the cost of the ionic liquid process must also be taken into consideration. Published by Elsevier Ltd. C1 [Li, Chenlin; Simmons, Blake A.; Singh, Seema] Sandia Natl Labs, Biomass Sci & Convers Technol Dept, Livermore, CA 94550 USA. [Li, Chenlin; Knierim, Bernhard; Manisseri, Chithra; Arora, Rohit; Scheller, Henrik V.; Auer, Manfred; Simmons, Blake A.; Singh, Seema] Univ Calif Berkeley, Lawrence Berkeley Lab, Joint BioEnergy Inst, Phys Biosci Div, Emeryville, CA USA. [Vogel, Kenneth P.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Singh, S (reprint author), Sandia Natl Labs, Biomass Sci & Convers Technol Dept, Livermore, CA 94550 USA. EM seesing@sandia.gov RI Scheller, Henrik/A-8106-2008; OI Scheller, Henrik/0000-0002-6702-3560; Li, Chenlin/0000-0002-0793-0505 FU US Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231] FX The authors thank Novozymes for the gift of the cellulase cocktails used in this work. The authors thank Dr. Shunlin Wang from Bruker Optics, Drs. Andreia M Smith and Lan Sun from the Joint BioEnergy Institute for the suggestions and discussion on FTIR and Raman analysis. This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, through Contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy. NR 42 TC 416 Z9 426 U1 26 U2 207 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 13 SI SI BP 4900 EP 4906 DI 10.1016/j.biortech.2009.10.066 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 585YT UT WOS:000276867500019 PM 19945861 ER PT J AU Zhu, JY Pan, XJ AF Zhu, J. Y. Pan, X. J. TI Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation SO BIORESOURCE TECHNOLOGY LA English DT Article DE Pretreatment; Woody/forest biomass; Enzymatic hydrolysis/saccharification; Cellulosic ethanol; Size reduction ID ENHANCED ENZYMATIC-HYDROLYSIS; COMPARATIVE SUGAR RECOVERY; SPENT SULFITE LIQUOR; BIOETHANOL PRODUCTION; STEAM PRETREATMENT; LODGEPOLE PINE; HYBRID POPLAR; CORN STOVER; CHIP SIZE; TECHNOECONOMIC EVALUATION AB This review presents a comprehensive discussion of the key technical issues in woody biomass pretreatment: barriers to efficient cellulose saccharification, pretreatment energy consumption, in particular energy consumed for wood-size reduction, and criteria to evaluate the performance of a pretreatment. A post-chemical pretreatment size-reduction approach is proposed to significantly reduce mechanical energy consumption. Because the ultimate goal of biofuel production is net energy output, a concept of pretreatment energy efficiency (kg/MJ) based on the total sugar recovery (kg/kg wood) divided by the energy consumption in pretreatment (MJ/kg wood) is defined. It is then used to evaluate the performances of three of the most promising pretreatment technologies: steam explosion, organosolv, and sulfite pretreatment to overcome lignocelluloses recalcitrance (SPORL) for softwood pretreatment. The present study found that SPORL is the most efficient process and produced highest sugar yield. Other important issues, such as the effects of lignin on substrate saccharification and the effects of pretreatment on high-value lignin utilization in woody biomass pretreatment, are also discussed. Published by Elsevier Ltd. C1 [Zhu, J. Y.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Zhu, J. Y.; Pan, X. J.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI USA. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM jzhu@fs.fed.us RI mei, chaoqun/B-9857-2011 NR 72 TC 307 Z9 320 U1 16 U2 158 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 13 SI SI BP 4992 EP 5002 DI 10.1016/j.biortech.2009.11.007 PG 11 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 585YT UT WOS:000276867500033 PM 19969450 ER PT J AU Widmer, W Zhou, WY Grohmann, K AF Widmer, Wilbur Zhou, Weiyang Grohmann, Karel TI Pretreatment effects on orange processing waste for making ethanol by simultaneous saccharification and fermentation SO BIORESOURCE TECHNOLOGY LA English DT Article DE Pretreatment; Steam explosion; Citrus; SSF; Ethanol ID DILUTE-ACID HYDROLYSIS; PEEL WASTE; SACCHAROMYCES-CEREVISIAE; ENZYMATIC-HYDROLYSIS; GALACTURONIC ACID; CORN STOVER; CELLULASE; PECTINASE; ENZYMES; SUGARS AB Citrus processing waste (CPW) pretreated under different times, pH and temperatures was investigated. Pretreatments at 160 degrees C for longer than 4 min with steam purging were needed to remove limonene, an inhibitor for fermentation, to below 0.1%. While hemicelluloses were solubilized well following all pretreatments at 160 degrees C, just 70% of the pectin was solubilized in natural CPW compared to over 80% after pretreatments using acid modified CPW (pH 2.8). Pretreatments at 160 degrees C on base modified CPW (initial pH 6.8) quickly destroyed pectin, had significantly lower dissolved solids, and were excessively viscous. Total sugars fermentable by Saccharomyces cerevisiae were not changed after pretreatment at 160 degrees C for up to 8 min in CPW between pH 2.2-8.2. Ethanol yields based on sugar content after enzymatic hydrolysis after 48 h simultaneous saccharification and fermentation ranged from 76% to 94%. Ethanol yields were slightly lower but not statistically different using base modified pretreatments. (C) 2010 Published by Elsevier Ltd. C1 [Widmer, Wilbur; Zhou, Weiyang] ARS, USDA, Citrus & Subtrop Prod Res Lab, Winter Haven, FL 33881 USA. [Grohmann, Karel] Renewable Spirits LLC, Delray Beach, FL USA. RP Widmer, W (reprint author), ARS, USDA, Citrus & Subtrop Prod Res Lab, 600 Ave S NW, Winter Haven, FL 33881 USA. EM widmer@ars.usda.gov FU CRADA [583K9581250] FX The authors would like to acknowledge Sandra Matlack, Jereca Scales and Tommy Long for their technical support with all the experiments and analyses. This research was supported in part by CRADA agreement 583K9581250. NR 30 TC 43 Z9 46 U1 1 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 14 BP 5242 EP 5249 DI 10.1016/j.biortech.2009.12.038 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 594ZS UT WOS:000277581100027 PM 20189803 ER PT J AU Digman, MF Shinners, KJ Casler, MD Dien, BS Hatfield, RD Jung, HJG Muck, RE Weimer, PJ AF Digman, Matthew F. Shinners, Kevin J. Casler, Michael D. Dien, Bruce S. Hatfield, Ronald D. Jung, Hans-Joachim G. Muck, Richard E. Weimer, Paul J. TI Optimizing on-farm pretreatment of perennial grasses for fuel ethanol production SO BIORESOURCE TECHNOLOGY LA English DT Article DE Pretreatment; Biomass; Switchgrass; Reed canarygrass; Ethanol ID CORN STOVER; SACCHAROMYCES-CEREVISIAE; SILO DENSITIES; ACID; ALFALFA; STORAGE; DIGESTIBILITY; SWITCHGRASS; DEGRADATION; TEMPERATURE AB Switchgrass (Panicum virgatum L) and reed canarygrass (Phalaris arundinacea L.) were pretreated under ambient temperature and pressure with sulfuric acid and calcium hydroxide in separate experiments. Chemical loadings from 0 to 100 g (kg DM)(-1) and durations of anaerobic storage from 0 to 180 days were investigated by way of a central composite design at two moisture contents (40% or 60% w.b.). Pretreated and untreated samples were fermented to ethanol by Saccharomyces cerevisiae D5A in the presence of a commercially available cellulase (Celluclast 1.5 L) and beta-glucosidase (Novozyme 188). Xylose levels were also measured following fermentation because xylose is not metabolized by S. cerevisiae. After sulfuric acid pretreatment and anaerobic storage, conversion of cell wall glucose to ethanol for reed canarygrass ranged from 22% to 83% whereas switchgrass conversions ranged from 16% to 46%. Pretreatment duration had a positive effect on conversion but was mitigated with increased chemical loadings. Conversions after calcium hydroxide pretreatment and anaerobic storage ranged from 21% to 55% and 18% to 54% for reed canarygrass and switchgrass, respectively. The efficacy of lime pretreatment was found to be highly dependent on moisture content. Moreover, pretreatment duration was only found to be significant for reed canarygrass. Although significant levels of acetate and lactate were observed in the biomass after storage, S. cerevisiae was not found to be inhibited at a 10% solids loading. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Digman, Matthew F.; Shinners, Kevin J.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Digman, Matthew F.; Casler, Michael D.; Hatfield, Ronald D.; Muck, Richard E.; Weimer, Paul J.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Dien, Bruce S.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Jung, Hans-Joachim G.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. RP Digman, MF (reprint author), Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. EM digman@wisc.edu OI Dien, Bruce/0000-0003-3863-6664 NR 35 TC 38 Z9 40 U1 0 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 14 BP 5305 EP 5314 DI 10.1016/j.biortech.2010.02.014 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 594ZS UT WOS:000277581100035 PM 20202834 ER PT J AU Anderson, RC Huwe, JK Smith, DJ Stanton, TB Krueger, NA Callaway, TR Edrington, TS Harvey, RB Nisbet, DJ AF Anderson, Robin C. Huwe, Janice K. Smith, David J. Stanton, Thaddeus B. Krueger, Nathan A. Callaway, Todd R. Edrington, Thomas S. Harvey, Roger B. Nisbet, David J. TI Effect of nitroethane, dimethyl-2-nitroglutarate and 2-nitro-methyl-propionate on ruminal methane production and hydrogen balance in vitro SO BIORESOURCE TECHNOLOGY LA English DT Article DE Methane-inhibitor; Dimethyl-2-nitroglutarate; 2-Nitro-methyl-propionate; Nitroethane; Rumen ID NITROPROPANOL-METABOLIZING BACTERIUM; FERMENTATION CHARACTERISTICS; SELECT NITROCOMPOUNDS; ORAL NITROETHANE; AMINO-ACIDS; RUMEN; MICROORGANISMS; FLUID; METHANOGENESIS; POPULATIONS AB Ruminal methanogenesis is considered a digestive inefficiency that results in the loss of 2-12% of the host's gross energy intake and contributes nearly 20% to the United States annual CH(4) emissions. Presently, the effects of the known CH(4) inhibitor, nitroethane, and two synthetic nitrocompounds, dimethyl-2-nitroglutarate and 2-nitro-methyl-propionate, on ruminal CH(4) production and fermentation were evaluated in vitro. After 24 h incubation at 39 degrees C under 100% CO(2), ruminal fluid cultures treated with 2.97 or 11.88 mu mol ml(-1) of the respective nitrocompounds produced >92% less CH(4) (P < 0.05) than non-treated controls. Quantification of fermentation end-products produced and H(2) balance estimates indicate that fermentation efficiencies were not compromised by the nitro-treatments. Published by Elsevier Ltd. C1 [Anderson, Robin C.; Krueger, Nathan A.; Callaway, Todd R.; Edrington, Thomas S.; Harvey, Roger B.; Nisbet, David J.] ARS, USDA, So Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Huwe, Janice K.; Smith, David J.] ARS, USDA, Biosci Res Lab, Fargo, ND 58105 USA. [Stanton, Thaddeus B.] ARS, USDA, Preharvest Food Safety & Enter Dis Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Anderson, RC (reprint author), ARS, USDA, So Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM Robin.Anderson@ars.usda.gov NR 34 TC 13 Z9 14 U1 1 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 14 BP 5345 EP 5349 DI 10.1016/j.biortech.2009.11.108 PG 5 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 594ZS UT WOS:000277581100040 PM 20194018 ER PT J AU Scordia, D Cosentino, SL Jeffries, TW AF Scordia, Danilo Cosentino, Salvatore L. Jeffries, Thomas W. TI Second generation bioethanol production from Saccharum spontaneum L. ssp aegyptiacum (Willd.) Hack. SO BIORESOURCE TECHNOLOGY LA English DT Article DE Pretreatment; Hemicellulose hydrolysate; Simultaneous saccharification; Ethanol ID PICHIA-STIPITIS; CORN STOVER; SIMULTANEOUS SACCHARIFICATION; HEMICELLULOSE HYDROLYSATE; ETHANOL-PRODUCTION; WHEAT-STRAW; PART 1; PRETREATMENT; BIOMASS; ENERGY AB Saccharum (Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. This study evaluated oxalic acid as a pretreatment for bioconversion. Overall sugar yields, sugar degradation products, enzymatic glucan hydrolysis and ethanol production were studied as effects of temperature (150-190 degrees C). reaction time (10-40 min) and oxalic acid concentration 2-8% (w/w). Time and temperature were combined into a single parameter, Severity Factor (SF) [Log (R(0))], and related to oxalic acid using a response surface methodology. Maximum total sugar yield was attained at a SF of 2.93 and 6.79% (w/w) oxalic acid, while maximum formation of sugar degradation products was observed at the highest SF (4.05) and 5% (w/w) oxalic acid. These were also the conditions for maximum simultaneous saccharification and fermentation (SSF) of the residual solids. Commercial cellulases and Saccharomyces cerevisiae attained 89.9% glucan conversion and 17.8 g/l ethanol. Pichia stipitis CBS 6054 fermented hemicellulosic hydrolysates from less severe conditions to ethanol with a yield of 0.35 (g(e)/g(s)). Maximal product yields were 69% of theoretical value and 90% of the SSF conversion efficiency for hydrolysate fermentation and SSF, respectively. Published by Elsevier Ltd. C1 [Scordia, Danilo; Jeffries, Thomas W.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Scordia, Danilo; Cosentino, Salvatore L.] Univ Catania, Sez Sci Agron, Dipartimento Sci Agron Agrochim & Prod Anim, I-95123 Catania, Italy. [Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Jeffries, TW (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM twjeffri@wisc.edu RI Jeffries, Thomas/I-8576-2012; Cosentino, Salvatore/F-7318-2011; OI Jeffries, Thomas/0000-0001-7408-4065; Cosentino, Salvatore/0000-0001-7076-8777; Scordia, Danilo/0000-0002-3822-788X FU Dipartimento di Scienze Agronomiche-DACPA; EdeniQ under USDA CRADA [08-RD-11111126-007] FX We thank Frederick J. Matt of the Analytical Chemistry and Microscopy Laboratory of the USDA Forest Products Laboratory (FPL) for carrying out the carbohydrate determination, Diane Dietrich (FPL) for conducting HMF and furfural analysis, and Steve Verrill (FPL) for helping with statistical analysis. This work was supported by Dipartimento di Scienze Agronomiche-DACPA and by EdeniQ under USDA CRADA 08-RD-11111126-007. NR 46 TC 29 Z9 29 U1 2 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 14 BP 5358 EP 5365 DI 10.1016/j.biortech.2010.02.036 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 594ZS UT WOS:000277581100042 PM 20194020 ER PT J AU Kim, Y Hendrickson, R Mosier, NS Ladisch, MR Bals, B Balan, V Dale, BE Dien, BS Cotta, MA AF Kim, Youngmi Hendrickson, Rick Mosier, Nathan S. Ladisch, Michael R. Bals, Bryan Balan, Venkatesh Dale, Bruce E. Dien, Bruce S. Cotta, Michael A. TI Effect of compositional variability of distillers' grains on cellulosic ethanol production SO BIORESOURCE TECHNOLOGY LA English DT Article DE Distillers' grains; DDGS; Ethanol; Inhibition; Xylose fermentation ID LIQUID HOT-WATER; DRIED GRAINS; ENZYMATIC-HYDROLYSIS; FERULIC ACID; SACCHAROMYCES YEAST; CORN STOVER; BY-PRODUCTS; MAIZE BRAN; SOLUBLES; FERMENTATION AB In a dry grind ethanol plant, approximately 0.84 kg of dried distillers' grains with solubles (DDGS) is produced per liter of ethanol. The distillers' grains contain the unhydrolyzed and unprocessed cellulosic fraction of corn kernels, which could be further converted to ethanol or other valuable bioproducts by applying cellulose conversion technology. Its compositional variability is one of the factors that could affect the overall process design and economics. In this study, we present compositional variability of distillers' grains collected from four different dry grind ethanol plants and its effect on enzymatic digestibility and fermentability. We then selected two sources of distillers grains based on their distinctive compositional difference. These were pretreated by either controlled pH liquid hot water (LHW) or ammonia fiber expansion (AFEX) and subjected to enzymatic hydrolysis and fermentation. Fermentation of the pretreated distillers' grains using either industrial yeast or genetically engineered glucose and xylose co-fermenting yeast, yielded 70-80% of theoretical maximum ethanol concentration, which varied depending on the batch of distillers' grains used. Results show that cellulose conversion and ethanol fermentation yields are affected by the compositions of distillers' grains. Distillers' grains with a high extractives content exhibit a lower enzymatic digestibility but a higher fermentability. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Kim, Youngmi; Hendrickson, Rick; Mosier, Nathan S.; Ladisch, Michael R.] Purdue Univ, Renewable Resources Engn Lab, Potter Engn Ctr, W Lafayette, IN 47907 USA. [Bals, Bryan; Balan, Venkatesh; Dale, Bruce E.] Michigan State Univ, Dept Chem Engn & Mat Sci, Biomass Convers Res Lab, E Lansing, MI 48824 USA. [Dien, Bruce S.; Cotta, Michael A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Ladisch, MR (reprint author), Purdue Univ, Renewable Resources Engn Lab, Potter Engn Ctr, 500 Cent Dr, W Lafayette, IN 47907 USA. EM ladisch@purdue.edu OI Cotta, Michael/0000-0003-4565-7754; Dien, Bruce/0000-0003-3863-6664 FU US Department of Energy [DE-FG36-04GO14220] FX This work was supported by US Department of Energy (Contract: DE-FG36-04GO14220) in cooperation with Midwest Consortium for Biobased Products and Bioenergy. We thank Genencor, a Danisco Division, for gifts of the enzymes used in this work and the dry grind plants for providing distillers' grains products. We also thank Drs. Nancy W. Ho and Miroslav Sedlak for providing the xylose co-fermenting Purdue yeast 424A (LNH-ST) and Ms. Patricia O'Bryan for technical help with the work done at NCAUR. We would like to thank Miroslav Sedlak and Eduardo Ximenes for their internal review of this paper and for helpful suggestions. NR 37 TC 20 Z9 21 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2010 VL 101 IS 14 BP 5385 EP 5393 DI 10.1016/j.biortech.2010.02.054 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 594ZS UT WOS:000277581100045 PM 20223655 ER PT J AU Scalley, TH Scatena, FN Lugo, AE Moya, S Ruiz, CRE AF Scalley, Tamara Heartsill Scatena, Fred N. Lugo, Ariel E. Moya, Samuel Ruiz, Carlos R. Estrada TI Changes in Structure, Composition, and Nutrients During 15 Yr of Hurricane-Induced Succession in a Subtropical Wet Forest in Puerto Rico SO BIOTROPICA LA English DT Article DE aboveground; biomass; Bisley Experimental Watersheds; Cecropia schreberiana; leaf chemistry; magnesium; secondary succession; species composition ID LUQUILLO-EXPERIMENTAL-FOREST; BISLEY EXPERIMENTAL WATERSHEDS; TROPICAL RAIN-FOREST; ABOVEGROUND BIOMASS; DISTURBANCE; HUGO; RESPONSES; FLUXES; DAMAGE; SOIL AB The trajectory of hurricane-induced succession was evaluated in a network of forest plots measured immediately before and 3 mo, 5, 10, and 15 yr after the direct impact of a Category 4 hurricane. Comparisons of forest structure, composition, and aboveground nutrients pools were made through time, and between species, life-history groups and geomorphic settings. The hurricane reduced aboveground biomass by 50 percent, causing an immediate decrease in stem density and diversity indices among all geomorphic settings. After 15 yr, basal area and aboveground biomass returned to pre-hurricane levels, while species richness, diversity indices, and stem densities exceeded pre-hurricane levels. Differences in species composition among geomorphic settings had not returned after 15 yr but differences in stem densities and structure were beginning to emerge. Significant differences were observed in the nutrient concentration of the three species that comprised the most aboveground biomass, and between species categorized as secondary high-light species and primary, low-light species. Species whose abundance was negatively correlated with the mature forest dominant also had distinct nutrient concentrations. When total aboveground nutrient pools were compared over time, differences in leaf nutrients among species were hidden by similarities in wood nutrient concentrations and the biomass dominance of a few species. The observed successional trajectory indicates that changes in species composition contributed to fast recovery of aboveground biomass and nutrient pools, while the influence of geomorphic setting on species composition occurs at time scales > 15 yr of succession. C1 [Scalley, Tamara Heartsill; Scatena, Fred N.; Lugo, Ariel E.; Moya, Samuel; Ruiz, Carlos R. Estrada] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. [Scalley, Tamara Heartsill; Scatena, Fred N.] Univ Penn, Dept Earth & Environm Sci, Philadelphia, PA 19104 USA. RP Scalley, TH (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. EM theartsill@fs.fed.us OI Heartsill Scalley, Tamara/0000-0003-0550-4147 FU National Science Foundation [BSR-8811764]; USDA FX This research was conducted in cooperation with the University of Puerto Rico under grant BSR-8811764 from the National Science Foundation to the Institute for Tropical Ecosystem Studies, University of Puerto Rico at Rio Piedras, and to the International Institute of Tropical Forestry, USDA Forest Service as part of the Long-Term Ecological Research Program (LTER) in the Luquillo Experimental Forest. The Department of Earth and Environmental Science, University of Pennsylvania, provided additional support. Earlier drafts of this manuscript received friendly reviews from J. D. Lodge, A. H. Johnson, and Y. A. Teh. The final version of the manuscript was improved by the suggestions and comments of R. Chazdon and two anonymous reviewers. NR 64 TC 18 Z9 18 U1 2 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3606 EI 1744-7429 J9 BIOTROPICA JI Biotropica PD JUL PY 2010 VL 42 IS 4 BP 455 EP 463 DI 10.1111/j.1744-7429.2009.00609.x PG 9 WC Ecology SC Environmental Sciences & Ecology GA 619OG UT WOS:000279438900009 ER PT J AU Cheng, DW Lin, H Takahashi, Y Walker, MA Civerolo, EL Stenger, DC AF Cheng, Davis W. Lin, Hong Takahashi, Yuri Walker, M. Andrew Civerolo, Edwin L. Stenger, Drake C. TI Transcriptional regulation of the grape cytochrome P450 monooxygenase gene CYP736B expression in response to Xylella fastidiosa infection SO BMC PLANT BIOLOGY LA English DT Article ID AVENA-SATIVA L.; RECEPTOR KINASE; ALTERNATIVE PROMOTERS; PIERCES-DISEASE; START SITES; ARABIDOPSIS; POLYADENYLATION; BIOSYNTHESIS; RESISTANCE; TOBACCO AB Background: Plant cytochrome P450 monooxygenases (CYP) mediate synthesis and metabolism of many physiologically important primary and secondary compounds that are related to plant defense against a range of pathogenic microbes and insects. To determine if cytochrome P450 monooxygenases are involved in defense response to Xylella fastidiosa (Xf) infection, we investigated expression and regulatory mechanisms of the cytochrome P450 monooxygenase CYP736B gene in both disease resistant and susceptible grapevines. Results: Cloning of genomic DNA and cDNA revealed that the CYP736B gene was composed of two exons and one intron with GT as a donor site and AG as an acceptor site. CYP736B transcript was up-regulated in PD-resistant plants and down-regulated in PD-susceptible plants 6 weeks after Xf inoculation. However, CYP736B expression was very low in stem tissues at all evaluated time points. 5'RACE and 3'RACE sequence analyses revealed that there were three candidate transcription start sites (TSS) in the upstream region and three candidate polyadenylation (PolyA) sites in the downstream region of CYP736B. Usage frequencies of each transcription initiation site and each polyadenylation site varied depending on plant genotype, developmental stage, tissue, and treatment. These results demonstrate that expression of CYP736B is regulated developmentally and in response to Xf infection at both transcriptional and post-transcriptional levels. Multiple transcription start and polyadenylation sites contribute to regulation of CYP736B expression. Conclusions: This report provides evidence that the cytochrome P450 monooxygenase CYP736B gene is involved in defense response at a specific stage of Xf infection in grapevines; multiple transcription initiation and polyadenylation sites exist for CYP736B in grapevine; and coordinative and selective use of transcription initiation and polyadenylation sites play an important role in regulation of CYP736B expression during growth, development and response to Xf infection. C1 [Cheng, Davis W.; Lin, Hong; Civerolo, Edwin L.; Stenger, Drake C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Cheng, Davis W.] Calif State Univ Fresno, Dept Biol, Fresno, CA 93740 USA. [Takahashi, Yuri; Walker, M. Andrew] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Takahashi, Yuri] Ehime Womens Coll, Dept Food Sci, Uwajima, Ehime 7980025, Japan. RP Lin, H (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM hong.lin@ars.usda.gov FU California Department of Food and Agriculture's Pierce's Disease and Glassy-winged Sharpshooter Board; USDA, Agricultural Research Service FX We gratefully acknowledge financial support from the California Department of Food and Agriculture's Pierce's Disease and Glassy-winged Sharpshooter Board and USDA, Agricultural Research Service. NR 44 TC 16 Z9 16 U1 1 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUL 1 PY 2010 VL 10 AR 135 DI 10.1186/1471-2229-10-135 PG 14 WC Plant Sciences SC Plant Sciences GA 637TD UT WOS:000280840700001 PM 20591199 ER PT J AU Johnstone, JF Chapin, FS Hollingsworth, TN Mack, MC Romanovsky, V Turetsky, M AF Johnstone, Jill F. Chapin, F. Stuart, III Hollingsworth, Teresa N. Mack, Michelle C. Romanovsky, Vladimir Turetsky, Merritt TI Fire, climate change, and forest resilience in interior Alaska SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID MIXEDWOOD BOREAL FOREST; BLACK SPRUCE FOREST; SOIL BURN SEVERITY; DISTURBANCE SEVERITY; ECOSYSTEM RESPONSES; TEMPORAL VARIATIONS; TAIGA ECOSYSTEMS; TREE RECRUITMENT; CARBON-DIOXIDE; CANADA AB In the boreal forests of interior Alaska, feedbacks that link forest soils, fire characteristics, and plant traits have supported stable cycles of forest succession for the past 6000 years. This high resilience of forest stands to fire disturbance is supported by two interrelated feedback cycles: (i) interactions among disturbance regime and plant-soil-microbial feedbacks that regulate soil organic layer thickness and the cycling of energy and materials, and (ii) interactions among soil conditions, plant regeneration traits, and plant effects on the environment that maintain stable cycles of forest community composition. Unusual fire events can disrupt these cycles and trigger a regime shift of forest stands from one stability domain to another (e. g., from conifer to deciduous forest dominance). This may lead to abrupt shifts in forest cover in response to changing climate and fire regime, particularly at sites with intermediate levels of moisture availability where stand-scale feedback cycles are only weakly constrained by environmental conditions. However, the loss of resilience in individual stands may foster resilience at the landscape scale, if changes in the landscape configuration of forest cover types feedback to stabilize regional patterns of fire behavior and climate conditions. C1 [Johnstone, Jill F.] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada. [Johnstone, Jill F.; Chapin, F. Stuart, III] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Hollingsworth, Teresa N.] Univ Alaska Fairbanks, USDA, US Forest Serv, PNW Res Stn,Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Mack, Michelle C.] Univ Florida, Dept Bot, Gainesville, FL 32610 USA. [Romanovsky, Vladimir] Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK 99775 USA. [Turetsky, Merritt] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 1G2, Canada. RP Johnstone, JF (reprint author), Univ Saskatchewan, Dept Biol, 112 Sci Pl, Saskatoon, SK S7N 5E2, Canada. EM jill.johnstone@usask.ca RI Johnstone, Jill/C-9204-2009; Turetsky, Merritt/B-1255-2013 OI Johnstone, Jill/0000-0001-6131-9339; FU NSF [DEB-0620579]; USDA Forest Service Pacific Northwest Research Station [PNW01-JV11261952-231]; US Joint Fire Sciences Program [05-1-2-06] FX This research was supported the Bonanza Creek Long-Term Ecological Research program (funded jointly by NSF grant DEB-0620579 and USDA Forest Service Pacific Northwest Research Station grant PNW01-JV11261952-231) and the US Joint Fire Sciences Program (project 05-1-2-06). We thank Sergei Marchenko for his contribution of data on soil temperatures from the Nome Creek study site and A. David McGuire and two anonymous reviewers for their helpful comments on previous versions of this paper. NR 66 TC 88 Z9 89 U1 13 U2 117 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUL PY 2010 VL 40 IS 7 BP 1302 EP 1312 DI 10.1139/X10-061 PG 11 WC Forestry SC Forestry GA 642JO UT WOS:000281208900009 ER PT J AU Chapin, FS McGuire, AD Ruess, RW Hollingsworth, TN Mack, MC Johnstone, JF Kasischke, ES Euskirchen, ES Jones, JB Jorgenson, MT Kielland, K Kofinas, GP Turetsky, MR Yarie, J Lloyd, AH Taylor, DL AF Chapin, F. S., III McGuire, A. D. Ruess, R. W. Hollingsworth, T. N. Mack, M. C. Johnstone, J. F. Kasischke, E. S. Euskirchen, E. S. Jones, J. B. Jorgenson, M. T. Kielland, K. Kofinas, G. P. Turetsky, M. R. Yarie, J. Lloyd, A. H. Taylor, D. L. TI Resilience of Alaska's boreal forest to climatic change SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID BLACK SPRUCE FORESTS; INTERIOR ALASKA; CARBON-CYCLE; FIRE; ECOSYSTEMS; SENSITIVITY; VULNERABILITY; PRODUCTIVITY; DISTURBANCE; FRUTICOSA AB This paper assesses the resilience of Alaska's boreal forest system to rapid climatic change. Recent warming is associated with reduced growth of dominant tree species, plant disease and insect outbreaks, warming and thawing of permafrost, drying of lakes, increased wildfire extent, increased postfire recruitment of deciduous trees, and reduced safety of hunters traveling on river ice. These changes have modified key structural features, feedbacks, and interactions in the boreal forest, including reduced effects of upland permafrost on regional hydrology, expansion of boreal forest into tundra, and amplification of climate warming because of reduced albedo (shorter winter season) and carbon release from wildfires. Other temperature-sensitive processes for which no trends have been detected include composition of plant and microbial communities, long-term landscape-scale change in carbon stocks, stream discharge, mammalian population dynamics, and river access and subsistence opportunities for rural indigenous communities. Projections of continued warming suggest that Alaska's boreal forest will undergo significant functional and structural changes within the next few decades that are unprecedented in the last 6000 years. The impact of these social-ecological changes will depend in part on the extent of landscape reorganization between uplands and lowlands and on policies regulating subsistence opportunities for rural communities. C1 [Chapin, F. S., III; Ruess, R. W.; Euskirchen, E. S.; Jones, J. B.; Kielland, K.; Taylor, D. L.] Univ Alaska Fairbanks, Inst Arctic Biol, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. [McGuire, A. D.] Univ Alaska Fairbanks, US Geol Survey, Alaska Cooperat Fish & Wildlife Unit, Fairbanks, AK 99775 USA. [Hollingsworth, T. N.] Univ Alaska Fairbanks, USDA, US Forest Serv, Pacific NW Res Stn,Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Mack, M. C.] Univ Florida, Dept Bot, Gainesville, FL 32611 USA. [Johnstone, J. F.] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada. [Kasischke, E. S.] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Jorgenson, M. T.] Alaska Ecosci, Fairbanks, AK 99709 USA. [Kofinas, G. P.] Univ Alaska Fairbanks, Sch Nat Resources & Agr Sci, Fairbanks, AK 99775 USA. [Turetsky, M. R.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 1G2, Canada. [Yarie, J.] Univ Alaska Fairbanks, Dept Forest Sci, Forest Soils Lab, Fairbanks, AK 99775 USA. [Lloyd, A. H.] Middlebury Coll, Dept Biol, Middlebury, VT 05753 USA. RP Chapin, FS (reprint author), Univ Alaska Fairbanks, Inst Arctic Biol, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. EM terry.chapin@alaska.edu RI Johnstone, Jill/C-9204-2009; Turetsky, Merritt/B-1255-2013; OI Johnstone, Jill/0000-0001-6131-9339; Chapin III, F Stuart/0000-0002-2558-9910 FU National Science Foundation [DEB-0620579]; USDA Forest Service, Pacific Northwest Research Station [PNW01-JV11261952-231] FX We thank the numerous Bonanza Creek LTER investigators and collaborators, including students and postdoctoral fellows, whose research led to the conclusions synthesized in this paper. The LTER program was funded jointly by National Science Foundation grant DEB-0620579 and USDA Forest Service, Pacific Northwest Research Station, grant PNW01-JV11261952-231. Other agencies that provided essential support included the Joint Fire Sciences Program, the National Atmospheric and Space Administration, and the US Geological Survey. NR 58 TC 52 Z9 53 U1 8 U2 109 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUL PY 2010 VL 40 IS 7 BP 1360 EP 1370 DI 10.1139/X10-074 PG 11 WC Forestry SC Forestry GA 642JO UT WOS:000281208900014 ER PT J AU Brose, PH Waldrop, TA AF Brose, Patrick H. Waldrop, Thomas A. TI A dendrochronological analysis of a disturbance-succession model for oak-pine forests of the Appalachian Mountains, USA SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID SOUTHWESTERN VIRGINIA; ICE STORMS; FIRE; PUNGENS; MAINTENANCE; INTENSITY; HISTORY; STANDS AB Disturbance-succession models describe the relationship between the disturbance regime and the dominant tree species of a forest type. Such models are useful tools in ecosystem management and restoration, provided they are accurate. We tested a disturbance-succession model for the oak-pine (Quercus spp. - Pinus spp.) forests of the Appalachian Mountains region using dendrochronological techniques. In this model, fire promotes pines, while fire suppression, bark beetle outbreaks, and ice storms encourage oaks. We analyzed nine Appalachian oak-pine stands for species establishment dates and the occurrence of fires and canopy disturbances. We found no evidence that fire preferentially promoted the establishment of pine more than oak, nor did we find any evidence that canopy disturbances or periods of no disturbance facilitated the establishment of oak more than pine. Rather, we found that both species groups originated primarily after combined canopy and fire disturbances, and reduction of fire frequency and scope coincided with the cessation of successful oak and pine regeneration. Currently, heath shrubs are slowly dominating these stands, so we present a revised disturbance-succession model for land managers struggling to manage or restore oak-pine forests containing a dense ericaceous understory. C1 [Brose, Patrick H.] US Forest Serv, No Res Stn, Forestry Sci Lab, Irvine, PA 16329 USA. [Waldrop, Thomas A.] Clemson Univ, US Forest Serv, So Res Stn, Clemson, SC 29634 USA. RP Brose, PH (reprint author), US Forest Serv, No Res Stn, Forestry Sci Lab, 335 Natl Forge Rd, Irvine, PA 16329 USA. EM pbrose@fs.fed.us FU Joint Fire Science Program FX This study would not have been possible without the help of many people, agencies, and organizations. First, we thank the Joint Fire Science Program for funding the Table Mountain Pine Project of which this study was part. We are grateful to the Chattahoochee National Forest, Great Smoky Mountains National Park, and Sumter National Forest for permission to conduct research on their lands and guidance in locating the study sites. We are especially indebted to Jamie Browning, Kelly Irwin, and Helen Mohr for their willingness and hard work to collect the cores and cross sections from rugged sites under inhospitable conditions. We also thank them, Wendy Andersen, and Greg Sanford for preparing the cores and cross sections for analysis. Special thanks go to Bryan Black, Bob Ford, and Jim Speer for guidance in cross-dating and conducting the many analyses. Finally, we thank Robert Long, Alex Royo, and two anonymous individuals for reviews that helped to clarify and shorten the manuscript. NR 50 TC 6 Z9 7 U1 3 U2 25 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUL PY 2010 VL 40 IS 7 BP 1373 EP 1385 DI 10.1139/X10-077 PG 13 WC Forestry SC Forestry GA 642JO UT WOS:000281208900015 ER PT J AU Ford, CR Minor, ES Fox, GA AF Ford, Chelcy R. Minor, Emily S. Fox, Gordon A. TI Long-term effects of fire and fire-return interval on population structure and growth of longleaf pine (Pinus palustris) SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID PRESCRIBED FIRE; UNITED-STATES; FLORIDA; ECOSYSTEMS; OVERSTORY; SAVANNAS; FORESTS; RESTORATION; DISTURBANCE; RESOURCES AB We investigated the effect of fire and fire frequency on stand structure and longleaf pine (Pinus palustris P. Mill.) growth and population demography in an experimental research area in a southwest Florida sandhill community. Data were collected from replicated plots that had prescribed fire-return intervals of 1, 2, 5, or 7 years or were left un-burned. Experimental treatment burns have been ongoing since 1976. Plots were sampled to estimate species distribution, stand structure, and longleaf pine density in four developmental stage classes: grass, bolting, small tree, and large tree. Tree-ring growth measurements in combination with burn history were used to evaluate the effects of fire and fire-return interval on basal area increment growth. Fire-return interval impacted stand structure and longleaf pine population structure. Our results suggest that recruitment from the bolting stage to later stages may become adversely affected with very frequent fires (e.g., every 1 or 2 years). Although adult tree productivity was negatively impacted during fire years, tree growth during years between fire events was resilient such that growth did not differ significantly among fire-return intervals. Our study shows that the longleaf pine population as a whole is strongly regulated by fire and fire-return interval plays a key role in structuring this population. C1 [Ford, Chelcy R.; Minor, Emily S.; Fox, Gordon A.] Univ S Florida, Dept Integrat Biol, Tampa, FL 33620 USA. RP Ford, CR (reprint author), US Forest Serv, USDA, SRS Coweeta Hydrol Lab, Otto, NC 28763 USA. EM crford@fs.fed.us OI Fox, Gordon/0000-0003-0595-1385; Miniat, Chelcy/0000-0002-3266-9783 FU National Science Foundation [DEB-9806923]; University of South Florida FX Funding was provided by the National Science Foundation, grant No. DEB-9806923, and by the University of South Florida. We thank Summer Solomon, Tracy Myhalyk, and Anne Schmidt for field assistance and data collection and Katherine Elliott and Rob Addington for comments on an earlier draft. NR 48 TC 4 Z9 4 U1 31 U2 130 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUL PY 2010 VL 40 IS 7 BP 1410 EP 1420 DI 10.1139/X10-080 PG 11 WC Forestry SC Forestry GA 642JO UT WOS:000281208900018 ER PT J AU Harrington, NP O'Rourke, KI Feng, YQ Rendulich, J DiFruscio, C Balachandran, A AF Harrington, Noel P. O'Rourke, Katherine I. Feng, Yuqin Rendulich, Jasmine DiFruscio, Cathleen Balachandran, Aru TI Prion genotypes of scrapie-infected Canadian sheep 1998-2008 SO CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE LA English DT Article ID PROTEIN GENE POLYMORPHISMS; PRP GENOTYPES; HEALTHY; BSE AB This report describes the genetics of the prion protein gene (PRNP) at codons 136, 154, and 171 for sheep diagnosed with naturally acquired classical scrapie in Canada between 1998 and 2008. Genotyping analysis was performed on 249 sheep with confirmed classical scrapie infection representing 98 flocks from 6 provinces. A further case-control analysis of 3 of these flocks compared the genotypes between infected sheep (n = 72) and those of their healthy flockmates (n = 1990). The incidence of classical scrapie in the Canadian sheep population was highly associated with the ARQ haplotype (91.8%) and the ARQ/ARQ genotype (91.6%). In addition, the ARQ haplotype was found at significantly higher frequency in scrapie-infected sheep when compared with their healthy flockmates. Comparison with other published data suggests that the scrapie risk of PRNP genotypes differs between Canada and countries where the VRQ allele is associated with the highest susceptibility to infection. C1 [Harrington, Noel P.; Feng, Yuqin; Rendulich, Jasmine; DiFruscio, Cathleen; Balachandran, Aru] Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Natl Reference Lab Scrapie & CWD, Ottawa, ON K2H 8P9, Canada. [O'Rourke, Katherine I.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. RP Harrington, NP (reprint author), Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Natl Reference Lab Scrapie & CWD, 3851 Fallowfield Rd, Ottawa, ON K2H 8P9, Canada. EM noel.harrington@inspection.gc.ca FU Science Division of the Canadian Food Inspection Agency [NTSE-04-04, NTSE-04-CIHR] FX This project was funded by the Science Division of the Canadian Food Inspection Agency (NTSE-04-04, NTSE-04-CIHR). The authors thank all of the sheep producers who participated in the study and all CFIA field staff involved in the collection and processing of samples. NR 16 TC 5 Z9 5 U1 0 U2 4 PU CANADIAN VET MED ASSOC PI OTTAWA PA 339 BOOTH ST ATTN: KIMBERLY ALLEN-MCGILL, OTTAWA, ONTARIO K1R 7K1, CANADA SN 0830-9000 J9 CAN J VET RES JI Can. J. Vet. Res.-Rev. Can. Rech. Vet. PD JUL PY 2010 VL 74 IS 3 BP 228 EP 232 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 699GM UT WOS:000285651900011 PM 20885849 ER PT J AU Gibson, TM Weinstein, SJ Mayne, ST Pfeiffer, RM Selhub, J Taylor, PR Virtamo, J Albanes, D Stolzenberg-Solomon, R AF Gibson, Todd M. Weinstein, Stephanie J. Mayne, Susan T. Pfeiffer, Ruth M. Selhub, Jacob Taylor, Philip R. Virtamo, Jarmo Albanes, Demetrius Stolzenberg-Solomon, Rachael TI A prospective study of one-carbon metabolism biomarkers and risk of renal cell carcinoma SO CANCER CAUSES & CONTROL LA English DT Article DE Folate; Renal cell carcinoma; Biological markers; Nested case-control study; B vitamins ID CANCER-RISK; UNITED-STATES; FOLATE INTAKE; PANCREATIC-CANCER; CIGARETTE-SMOKING; BLOOD FOLATE; VEGETABLES; PLASMA; HOMOCYSTEINE; FRUITS AB Previous studies have found associations between one-carbon metabolism factors and risk of several cancers, but little is known regarding renal cell carcinoma (RCC). We conducted a nested case-control study within the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study, a prospective study of Finnish male smokers aged 50-69 at baseline. Prediagnostic folate, vitamin B(6), vitamin B(12), cysteine, riboflavin, and homocysteine concentrations were measured in fasting serum from 224 incident RCC cases and 224 controls (matched on age and date of serum collection). Conditional logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (CIs), adjusted for potential confounders. Serum folate tended to be inversely associated with RCC, compared to the first quartile, the odds ratios (95% CI) for subsequent quartiles were 0.62 (0.35-1.08), 0.52 (0.29-0.93), and 0.67 (0.37-1.20) (P-trend = 0.19). When modeled as a threshold effect, subjects in the lowest serum folate quartile (a parts per thousand currency sign6.64 nmol/l), which corresponds to deficient folate status, had a significant increased RCC risk (OR = 1.68, 95% CI 1.06-2.65) compared to those with higher serum folate. The other one-carbon metabolism biomarkers were not associated with RCC. This study in male smokers suggests that deficient folate status may increase risk of RCC, but confirmation is needed in other epidemiologic studies that include women and non-smokers. C1 [Gibson, Todd M.; Weinstein, Stephanie J.; Pfeiffer, Ruth M.; Taylor, Philip R.; Albanes, Demetrius; Stolzenberg-Solomon, Rachael] NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Rockville, MD 20852 USA. [Gibson, Todd M.; Mayne, Susan T.] Yale Univ, Sch Publ Hlth, New Haven, CT 06520 USA. [Selhub, Jacob] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Virtamo, Jarmo] Natl Publ Hlth Inst, Dept Hlth Promot & Chron Dis Prevent, Helsinki, Finland. RP Gibson, TM (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, 6120 Execut Blvd, Rockville, MD 20852 USA. EM gibsontm@mail.nih.gov RI Albanes, Demetrius/B-9749-2015 FU NIH, National Cancer Institute [TU2-CA-105666]; National Cancer Institute, Department of Health and Human Services [N01-CN-45165, N01-RC-45035, N01-RC-37004] FX This research was supported in part by the Intramural Research Program of the NIH and the National Cancer Institute and by grant TU2-CA-105666. Additionally, this research was supported by Public Health Service contracts N01-CN-45165, N01-RC-45035, and N01-RC-37004 from the National Cancer Institute, Department of Health and Human Services. NR 46 TC 15 Z9 16 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0957-5243 J9 CANCER CAUSE CONTROL JI Cancer Causes Control PD JUL PY 2010 VL 21 IS 7 BP 1061 EP 1069 DI 10.1007/s10552-010-9534-5 PG 9 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA 608WE UT WOS:000278615900009 PM 20383577 ER PT J AU Paul, S DeCastro, AJ Lee, HJ Smolarek, AK So, JY Simi, B Wang, CX Zhou, RP Rimando, AM Suh, N AF Paul, Shiby DeCastro, Andrew J. Lee, Hong Jin Smolarek, Amanda K. So, Jae Young Simi, Barbara Wang, Chung Xiou Zhou, Renping Rimando, Agnes M. Suh, Nanjoo TI Dietary intake of pterostilbene, a constituent of blueberries, inhibits the beta-catenin/p65 downstream signaling pathway and colon carcinogenesis in rats SO CARCINOGENESIS LA English DT Article ID ABERRANT CRYPT FOCI; FAMILIAL ADENOMATOUS POLYPOSIS; OXIDE SYNTHASE INHIBITOR; DEPENDENT KINASE 4; CYCLIN D1; BETA-CATENIN; KAPPA-B; CARCINOMA CELLS; CANCER CELLS; RESVERATROL AB Stilbenes are phytochemicals present in grapes, berries, peanuts and red wine. A widely studied stilbene, resveratrol (trans-3,5,4'-trihydroxystilbene), has been shown to exert antioxidant, anti-inflammatory, chemopreventive and antiaging effects in a number of biological systems. We reported earlier that pterostilbene (trans-3,5-dimethoxy-4'-hydroxystilbene), a structurally related stilbene found in blueberries, was effective in reducing the incidence and multiplicity of aberrant crypt foci formation in the colon of rats injected with azoxymethane (AOM). Our present study was to identify the chemopreventive potential of pterostilbene with colonic tumor formation as an end point and further to evaluate the mechanistic action of pterostilbene during colon carcinogenesis. F344 rats were given two AOM injections subcutaneously when they were 7 and 8 weeks old and continuously fed the control or 40 p.p.m. pterostilbene diet for 45 weeks. Overall analyses indicated that pterostilbene reduced colon tumor multiplicity of non-invasive adenocarcinomas, lowered proliferating cell nuclear antigen and downregulated the expression of beta-catenin and cyclin D1. Pterostilbene decreased mucosal levels of the proinflammatory cytokines, tumor necrosis factor-alpha, interleukin (IL)-1 beta and IL-4. Colon tumors from pterostilbene-fed animals showed reduced expression of inflammatory markers as well as nuclear staining for phospho-p65, a key molecule in the nuclear factor-kappaB pathway. In HT-29 cells, pterostilbene reduced the protein levels of beta-catenin, cyclin D1 and c-MYC, altered the cellular localization of beta-catenin and inhibited the phosphorylation of p65. Our data with pterostilbene in suppressing colon tumorigenesis, cell proliferation as well as key inflammatory markers in vivo and in vitro suggest the potential use of pterostilbene for colon cancer prevention. C1 [Paul, Shiby; DeCastro, Andrew J.; Lee, Hong Jin; Smolarek, Amanda K.; So, Jae Young; Simi, Barbara; Wang, Chung Xiou; Zhou, Renping; Suh, Nanjoo] Rutgers State Univ, Ernest Mario Sch Pharm, Dept Biol Chem, Piscataway, NJ 08854 USA. [Rimando, Agnes M.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Suh, Nanjoo] Canc Inst New Jersey, New Brunswick, NJ 08903 USA. RP Suh, N (reprint author), Rutgers State Univ, Ernest Mario Sch Pharm, Dept Biol Chem, 164 Frelinghuysen Rd, Piscataway, NJ 08854 USA. EM nsuh@rci.rutgers.edu RI Sanguansri, Luz/B-6630-2011 OI Sanguansri, Luz/0000-0003-1908-7604 FU National Institute of Environmental and Health Sciences [P30 ES005022]; State University of New Jersey, Rutgers FX National Institute of Environmental and Health Sciences (P30 ES005022); Trustees Research Fellowship Program at Rutgers, The State University of New Jersey. NR 50 TC 69 Z9 69 U1 2 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD JUL PY 2010 VL 31 IS 7 BP 1272 EP 1278 DI 10.1093/carcin/bgq004 PG 7 WC Oncology SC Oncology GA 620BD UT WOS:000279473100014 PM 20061362 ER PT J AU Wong, W Barba, P Alvarez, C Castro, A Acuna, M Zamora, P Rosales, M Dell'Orto, P Moynihan, MR Scorza, R Prieto, H AF Wong, Wendy Barba, Paola Alvarez, Catalina Castro, Alvaro Acuna, Manuel Zamora, Pablo Rosales, Marlene Dell'Orto, Paola Moynihan, Michael R. Scorza, Ralph Prieto, Humberto TI EVALUATION OF THE RESISTANCE OF TRANSGENIC C5 PLUM (Prunus domestica L.) AGAINST FOUR CHILEAN PLUM POX VIRUS ISOLATES THROUGH MICRO-GRAFTING SO CHILEAN JOURNAL OF AGRICULTURAL RESEARCH LA English DT Article DE C5; Plum pox virus; Chilean isolates; Prunus domestica; Prunus insititia; micro-grafting ID GENE; ROOTSTOCK; GROWTH; CLONES AB The transgenic plum (Prunus domestica L.) C5, in which the coat protein (CP) gene of the Plum pox virus (PPV) is inserted, represents a unique example of the use of genetic engineering for fruit crop improvement in Prunus spp. Field trials in Poland, Romania, and Spain have demonstrated resistance of C5 to several D and M strain PPV isolates. In Chile, the quarantine regulations for PPV and for genetically modified (GM) plants require that the testing of C5 for resistance to Chilean PPV isolates be done under controlled isolated conditions. To carry out these tests C5 shoots were multiplied in vitro and micro-grafted onto four Adesoto101 (Prunus insititia L.) rootstock populations that had been previously infected each with one of four Chilean PPV-Ds. Tests were carried out under controlled conditions in a biosafety greenhouse. Symptoms appearance, virus detection, and viral mRNA levels for the cylindrical inclusion (CI) and CP genes were determined during three consecutive growing seasons. Complete resistance to all PPV isolates was demonstrated during the first 2 yr in all of the C5 plants. In the third season, four of 10 C5 plants showed mild symptoms on leaves close to the graft union and low but detectable CI mRNA levels in the C5 scions. These results support the effectiveness of using of micro-grafting on P. insititia for PPV resistance studies, especially in the limited space of a quarantine facility; whereas resistance levels in C5 after 3 yr indicate the importance of long term and field scale evaluations. C1 [Barba, Paola; Alvarez, Catalina; Acuna, Manuel; Rosales, Marlene; Prieto, Humberto] Inst Invest Agropecuarias INIA, Santiago, Chile. [Wong, Wendy] Univ Chile, Fac Ciencias Agron, Santiago, Chile. [Castro, Alvaro; Zamora, Pablo] Univ Santiago Chile, Fac Quim & Biol, Santiago, Chile. [Dell'Orto, Paola; Moynihan, Michael R.] Fdn Chile, Santiago, Chile. [Scorza, Ralph] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Prieto, H (reprint author), Inst Invest Agropecuarias INIA, POB 549, Santiago, Chile. EM hprieto@inia.cl RI Zamora, Pablo/D-1612-2012; Prieto, Humberto/B-4400-2008 OI Prieto, Humberto/0000-0002-9013-1906 FU FDI-CORFO-Chile [02LE2-0005]; Biotecnologia Fruticola S.A./Biofrutales (Programa Bicentenario de Ciencia y Tecnologia, PBCT-Chile) FX We thank to Dr. Michel Ravelonandro (Institut National de la Recherche Agronomique, INRA-Bordeaux), and Nicola Fiore (Universidad de Chile), for their critical review of this manuscript and the collaboration in the handling of PPV isolates, respectively. This work was funded by grants FDI-CORFO-Chile 02LE2-0005 and Biotecnologia Fruticola S.A./Biofrutales (Programa Bicentenario de Ciencia y Tecnologia, PBCT-Chile). NR 24 TC 4 Z9 4 U1 0 U2 11 PU INST INVESTIGACIONES AGROPECUARIAS PI CHILLAN PA CENTRO REGIONAL DE INVESTIGACION QUILAMAPU, CASILLA 426, CHILLAN, 00000, CHILE SN 0718-5820 J9 CHIL J AGR RES JI Chil. J. Agric. Res. PD JUL-SEP PY 2010 VL 70 IS 3 BP 372 EP 380 PG 9 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA 674CH UT WOS:000283710800004 ER PT J AU Caperna, TJ Shannon, AE Blomberg, LA Garrett, WM Ramsay, TG AF Caperna, Thomas J. Shannon, Amy E. Blomberg, Le Ann Garrett, Wesley M. Ramsay, Timothy G. TI Identification of protein carbonyls in serum of the fetal and neonatal pig SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY LA English DT Article DE 2D-PAGE; MALDI-TOF-MS; LCMS/MS; Protein oxidation; Runts ID OXIDATIVE STRESS; MASS-SPECTROMETRY; GROWTH; PLASMA; RESTRICTION; BIRTH AB Oxidation of serum proteins leads to non-reversible carbonyl formation which alters their function and is associated with stress-related disease processes. The primary objective of this study was to quantify and identify oxidized serum proteins in fetal and newborn piglets Protein carbonyls were converted to hydrazones with dinitrophenyl hydrazine and quantified spectrophotometrically. For identification, serum protein carbonyls were derivatized with biotin hydrazide, separated by 2D PAGE and stained with FITC-avidin Biotin-labeled proteins were excised from gels and identified by mass spectrometry At birth. carbonyls were determined to be similar to 600 pmole/mg serum protein Fetuses at 50 and 100 clays of gestation had similar levels of protein carbonyls as newborns. Carbonyl levels were also similar for control and runt (<1 kg at birth) piglets between 1 and 21 days of age, however, distribution of many proteins varied by age and was also influenced by birth weight. Major oxidized proteins identified in fetal (f) and newborn (n) pigs included, albumin (f, n), transferrin (f. n). fetuin-A (f, n) alpha fetoprotein (f, n). plasminogen (f, n), fetuin-B (f). alpha-1-antitrypsin (f, n) alpha-1-acid glycoprotein (f) and immunoglobulins (n) While abundance and distribution of oxidized proteins changed over time, these changes appear to primarily reflect relative amounts of those proteins in serum. Published by Elsevier Inc C1 [Caperna, Thomas J.; Shannon, Amy E.; Blomberg, Le Ann; Garrett, Wesley M.; Ramsay, Timothy G.] USDA ARS, Anim Biosci & Biotechnol Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Caperna, TJ (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Bldg 200,Rm 202,BARC E, Beltsville, MD 20705 USA. NR 22 TC 15 Z9 15 U1 2 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1096-4959 J9 COMP BIOCHEM PHYS B JI Comp. Biochem. Physiol. B-Biochem. Mol. Biol. PD JUL PY 2010 VL 156 IS 3 BP 189 EP 196 DI 10.1016/j.cbpb.2010.03.006 PG 8 WC Biochemistry & Molecular Biology; Zoology SC Biochemistry & Molecular Biology; Zoology GA 609NZ UT WOS:000278664900006 PM 20350615 ER PT J AU Thomson, SJ Huang, YB Hanks, JE Martin, DE Smith, LA AF Thomson, Steven J. Huang, Yanbo Hanks, James E. Martin, Daniel E. Smith, Lowrey A. TI Improving flow response of a variable-rate aerial application system by interactive refinement SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Aerial application; Automatic flow control; Precision agriculture; Site-specific management; Variable-rate application AB Experiments were conducted to evaluate response of a variable-rate aerial application controller to changing flow rates and to improve its response at correspondingly varying system pressures. System improvements have been made by refinement of the control algorithms over time in collaboration with the system manufacturer, Houma Avionics, Houma, LA, USA. The variable-rate application system consists of Differential Global Positioning System (DGPS)-based guidance, AutoCal II automatic flow controller, and hydraulically controlled spray pump. The AutoCal II was evaluated for its ability to track desired flow rates set by the pilot. The system was then evaluated over several field trials to quantify its response to rapidly changing flow requirements and to determine the effect of the latest control algorithm improvements on response characteristics. System responses were analyzed while operating the AutoCal II in automatic mode over a pre-set field prescription containing four management zones (28, 47, 56, and 37 L ha(-1) each 81 m long). To evaluate the effect of control algorithm improvements, areas under the flowrate-time curves were integrated and percentage differences in areas between those response curves and target flow rate curves were determined. Results for south-north runs indicated reduction of average error from 6.9% before control algorithm modification to 1.8% after algorithm modification. Benefits of a new flow monitor with capabilities for improved data acquisition resolution were illustrated by examining data from both 2005 and 2008 prescription runs. For the 2005 data, integration times per run matched expected values based on ground speed when using either the new flowmeter monitor or conventional monitoring via the AutoCal II with its irregular data integration intervals. The 2008 data showed inconsistencies in total integration time per run when reading flowmeter data via the AutoCal II: these intervals varied between 1.2 and 1.66s. Integration timing intervals matched expected results when using the new flowmeter monitor instead of the AutoCal II to read and output data. Inconsistencies in AutoCal II timing were attributed to possible modifications in loop-timing portions of the AutoCal II control algorithm since the 2005 run was conducted. These results further support the value of the new flowmeter monitor in providing consistent results regardless of changes the manufacturer might make to the AutoCal II data acquisition and control program. The experiments served to illustrate an example of how iterative refinement of control algorithms in collaboration with the control system manufacturer could improve system response characteristics. System evaluation techniques described should also apply to aircraft that use propeller-driven spray pumps as well as hydraulically controlled spray pumps. Published by Elsevier B.V. C1 [Thomson, Steven J.; Huang, Yanbo; Hanks, James E.; Smith, Lowrey A.] ARS, USDA, CPSRU, Stoneville, MS 38776 USA. [Martin, Daniel E.] ARS, USDA, APMRU, College Stn, TX USA. RP Thomson, SJ (reprint author), ARS, USDA, CPSRU, POB 350,141 Expt Stn Rd, Stoneville, MS 38776 USA. EM steve.thomson@ars.usda.gov NR 8 TC 5 Z9 6 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUL PY 2010 VL 73 IS 1 BP 99 EP 104 DI 10.1016/j.compag.2010.04.009 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 618RG UT WOS:000279372800011 ER PT J AU Kise, M Park, B Heitschmidt, GW Lawrence, KC Windham, WR AF Kise, Michio Park, Bosoon Heitschmidt, Gerald W. Lawrence, Kurt C. Windham, William R. TI Multispectral imaging system with interchangeable filter design SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Multispectral imaging system; Optical system design; Interchangeable filter; Image calibration AB The design and calibration of a three-band image acquisition system was reported in this paper. The prototype system developed in this research was a three-band spectral imaging system that acquired two visible-band images and one NIR image simultaneously. This was accomplished by using a three-port imaging system that consisted of three identical monochrome cameras, an optical system, and three interchangeable optical filters. Spectral reflectance from an object was collimated by a front lens, and split in three ways by a cold mirror and beamsplitter: a cold mirror reflects 90% visible light and transmits 80% NIR light. The visible light was again split identically into two directions by an additional beamsplitter. Focusing lenses then projected each image onto its corresponding sensor. By incorporating an interchangeable filter design, the imaging system can measure any two visible spectral bands that range between 400 nm and 700 nm, and one NIR band that ranges between 700 nm and 1000 nm without any complicated manufacturing process. In order to co-register the three images, a system-specific calibration algorithm was developed that compensates for lens-sensor geometric misalignments. The prototype imaging system and the system calibration algorithm were tested and evaluated for image alignment accuracy. The imaging system acquired three-band images of 3D objects with 0.39 pixel misalignment error on average. (C) 2010 Elsevier B.V. All rights reserved. C1 [Kise, Michio] John Deere Technol Innovat Ctr, Champaign, IL 61820 USA. [Park, Bosoon; Heitschmidt, Gerald W.; Lawrence, Kurt C.; Windham, William R.] USDA ARS, Athens, GA 30605 USA. RP Kise, M (reprint author), John Deere Technol Innovat Ctr, 2021 S 1st St,Suite 101, Champaign, IL 61820 USA. EM KiseMichio@JohnDeere.com; bosoon.park@ars.usda.gov; jerry.heitschmidt@ars.usda.gov; kurt.lawrence@ars.usda.gov; bob.windham@ars.usda.gov NR 13 TC 11 Z9 12 U1 1 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUL PY 2010 VL 72 IS 2 BP 61 EP 68 DI 10.1016/j.compag.2010.02.005 PG 8 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 610GW UT WOS:000278720700002 ER PT J AU Medeiros, FHV Pomella, AWV de Souza, JT Niella, GR Valle, R Bateman, RP Fravel, D Vinyard, B Hebbar, PK AF Medeiros, F. H. V. Pomella, A. W. V. de Souza, J. T. Niella, G. R. Valle, R. Bateman, R. P. Fravel, D. Vinyard, B. Hebbar, P. K. TI A novel, integrated method for management of witches' broom disease in Cacao in Bahia, Brazil SO CROP PROTECTION LA English DT Article DE Biological control; Mycoparasites; Tricoderma; Integrated management ID COCOA POD DISEASES; TRICHODERMA-STROMATICUM; THEOBROMA-CACAO; CAUSAL AGENTS; MYCOPARASITE; GERMINATION; PATHOGENS; ROT AB A three-year field study was conducted in Bahia, Brazil to validate several strategies for management of witches' broom disease in cacao caused by Moniliophthora perniciosa. Treatments which were applied alone or in combination included applications of biological control fungus Trichoderma stromaticum, fungicide copper hydroxide and phytosanitary broom removal. When compared with untreated control treatments, higher pod yields and consistently lower pod losses were obtained by alternating fungicide with biocontrol application. Pod losses caused by witches' broom were also reduced by fungicide treatment or by phytosanitary broom removal when applied alone or in combination, however total pod production per tree was consistently low whenever broom removal was used as a management strategy. While application of biocontrol fungus alone was not able to reduce witches' broom on pods, it reduced vegetative broom formation and also increased the number of pod-forming flower cushions. The present study indicates that alternating fungicide copper hydroxide with biocontrol fungus T. stromaticum without expensive phytosanitary broom removal is not only a better disease management strategy in Bahia but also results in better yields and thereby better net economic returns. Published by Elsevier Ltd. C1 [Medeiros, F. H. V.; Pomella, A. W. V.] Almirante Cacau Agr Comercio & Exportacao Ltda, BR-45630000 Itajuipe, BA, Brazil. [de Souza, J. T.; Niella, G. R.; Valle, R.] CEPLAC CEPEC, BR-45600000 Itabuna, BA, Brazil. [Bateman, R. P.] Univ London Imperial Coll Sci Technol & Med, IPARC, Ascot SL5 7PY, Berks, England. [Fravel, D.] USDA ARS, Vegetable Lab, Beltsville, MD 20705 USA. [Vinyard, B.] USDA ARS, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Hebbar, P. K.] USDA ARS, Inst Plant Sci, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. RP Hebbar, PK (reprint author), USDA APHIS PPQ, Emergency Program, 5C-03-40 4700 River Rd, Riverdale, MD USA. EM prakash.hebbar@aphis.usda.gov FU Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB); USDA; cacao sustainability initiative of Mars Inc. FX The work was supported by Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), providing financial support for the trials and assistantship for the first author. Various additional aspects of this work have been sponsored by USDA and the cacao sustainability initiative of Mars Inc. Our sponsors support policies, programmes and projects to promote international development and have provided funds for this study as part of that objective. However, the opinions expressed are those of the authors alone; trade names and commercial products are described in this article solely for the purpose of providing specific information and neither the authors nor our sponsors imply recommendation or their endorsement. NR 36 TC 8 Z9 9 U1 1 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD JUL PY 2010 VL 29 IS 7 BP 704 EP 711 DI 10.1016/j.cropro.2010.02.006 PG 8 WC Agronomy SC Agriculture GA 618ON UT WOS:000279365500010 ER PT J AU Ling, KS Jackson, DM Harrison, H Simmons, AM Pesic-VanEsbroeck, Z AF Ling, Kai-Shu Jackson, D. Michael Harrison, Howard Simmons, Alvin M. Pesic-VanEsbroeck, Zvezdana TI Field evaluation of yield effects on the USA heirloom sweetpotato cultivars infected by Sweet potato leaf curl virus SO CROP PROTECTION LA English DT Article DE Begomovirus; Ipomoea; Bemisia tabaci; Real-time PCR; Meristem tip culture; SPLCV ID FEATHERY-MOTTLE-VIRUS; BEMISIA-ARGENTIFOLII HOMOPTERA; COASTAL SOUTH-CAROLINA; CHLOROTIC-STUNT-VIRUS; STORAGE ROOT QUALITY; 1ST REPORT; UNITED-STATES; MOSAIC-VIRUS; DISEASE SPVD; BEAUREGARD AB The incidence of Sweet potato leaf curl virus (SPLCV), a Begomovirus, on sweetpotato, Ipomoea batatas (L) Lam. (Convolvulaceae), in South Carolina, U.S.A. has increased rapidly in recent years. This is likely due to the use of infected propagating materials and the increasing population of its vector, the sweetpotato whitefly, Bemisia tabaci (Gennadius). In this study, we demonstrated in field experiments that SPLCV infection reduced the yields of most heirloom sweetpotato lines relative to the yields of noninfected plants. Real-time polymerase chain reaction (PCR) technology specific to several common sweetpotato viruses was used to determine the virus infection status in 69 selected accessions of heirloom sweetpotato lines. Meristem tip culture technology was used to regenerate virus-free plants from these materials. To ensure the virus-free status, each mericlone was evaluated using real-time PCR and graft bio-indexing on the indicator species, Ipomoea setosa Ker Gawl. Mericlones of 27 cultivars were found to be free of the viruses. The 27 cultivars were included in a field test to determine the effect of SPLCV infection on yield. Yields of virus-free plants of the cultivars ranged from 10 to 80% greater than the yields of SPLCV-infected plants. However, the yield differences between virus-free and infected plants were diminished in the second year of the field experiment due to a rapid re-infection by SPLCV. These results demonstrate the importance of using certified, virus-tested seed roots or cuttings. The rapid re-infection of the virus-tested sweetpotato plants with SPLCV observed in these studies suggests that management of the whitefly population should be a critical element in control of this important virus. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Ling, Kai-Shu; Jackson, D. Michael; Harrison, Howard; Simmons, Alvin M.] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Pesic-VanEsbroeck, Zvezdana] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. RP Ling, KS (reprint author), ARS, US Vegetable Lab, USDA, 2700 Savannah Highway, Charleston, SC 29414 USA. EM kai.ling@ars.usda.gov FU USDA FX We thank Christopher Clark, Patrick Wechter and Amnon Levi for valuable discussion and critical reviews of the manuscript. Andrea Gilliard, Emily Walters, Eric Marom, Raymond Jacobs, Jennifer Cook, Lance Lawrence, and Ty Phillips provided excellent technical assistance. Plant materials were provided by USDA, Plant Genetic Resources Conservation Unit in Griffin, GA. Funding for this research was supported in part by USDA as recommended by the Sweetpotato Crop Germplasm Committee. NR 66 TC 14 Z9 17 U1 6 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD JUL PY 2010 VL 29 IS 7 BP 757 EP 765 DI 10.1016/j.cropro.2010.02.017 PG 9 WC Agronomy SC Agriculture GA 618ON UT WOS:000279365500018 ER PT J AU Ellis, DD Garland-Campbell, KA Grotenhuis, JA Jenderek, MM Pedersen, JF AF Ellis, D. D. Garland-Campbell, K. A. Grotenhuis, J. A. Jenderek, M. M. Pedersen, J. F. TI Crop Registration: The Pathway to Public Access of Plant Genetic Materials to Build Crops for the Future SO CROP SCIENCE LA English DT Article ID MAPPING POPULATION; PARENTAL LINES; MAIZE AB Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange such as Intellectual Property Rights (IPR) and international treaties (i.e., The International Treaty on Genetic Resources for Food and Agriculture). Together with (i) the advent of the new Journal of Plant Registrations; (ii) the facilitated search capabilities of GRIN; (iii) the development and definition of genetic materials needing registration (cultivars, germplasm, genetic stocks, parental lines, and mapping populations); and (iv) the recognition and allowance of IPR rights. the registration of crops is a healthy viable dynamic registration system to meet the needs of breeders over the next 80 yr. C1 [Ellis, D. D.; Grotenhuis, J. A.; Jenderek, M. M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. [Garland-Campbell, K. A.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Pedersen, J. F.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Ellis, DD (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. NR 51 TC 1 Z9 1 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1151 EP 1160 DI 10.2135/cropsci2009.10.0585 PG 10 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200006 ER PT J AU Campbell, BT Saha, S Percy, R Frelichowski, J Jenkins, JN Park, W Mayee, CD Gotmare, V Dessauw, D Giband, M Du, X Jia, Y Constable, G Dillon, S Abdurakhmonov, IY Abdukarimov, A Rizaeva, SM Adullaev, A Barroso, PAV Padua, JG Hoffmann, LV Podolnaya, L AF Campbell, B. T. Saha, S. Percy, R. Frelichowski, J. Jenkins, J. N. Park, W. Mayee, C. D. Gotmare, V. Dessauw, D. Giband, M. Du, X. Jia, Y. Constable, G. Dillon, S. Abdurakhmonov, I. Y. Abdukarimov, A. Rizaeva, S. M. Adullaev, A. Barroso, P. A. V. Padua, J. G. Hoffmann, L. V. Podolnaya, L. TI Status of the Global Cotton Germplasm Resources SO CROP SCIENCE LA English DT Article ID GENETIC DIVERSITY; GOSSYPIUM-TOMENTOSUM; COLLECTION; INTROGRESSION; MARKERS; IDENTIFICATION; VULNERABILITY; CONSERVATION; ASSOCIATION; PERFORMANCE AB The cultivated Gossypium spp. (cotton) represents the single most important, natural fiber crop in the world. In addition to its fiber, the oil and protein portion of the cottonseed also represents significant economic value. To protect the worldwide economic value of cotton fiber and cotton byproducts, coordinated efforts to collect and maintain cotton genetic resources have increased over the last 100 yr. The classified genetic resources of cotton are extensive and include five tetraploid species in the primary gene pool, 20 diploid species in the secondary gene pool, and 25 diploid species in the tertiary gene pool. This report provides information on the status and contents of eight major cotton germplasm collections present across the world. Based on the findings of this report, a number of classified Gossypium species are not maintained in these collections, and several are underrepresented and vulnerable to extinction. This report presents several critical challenges and opportunities facing international efforts to enhance and preserve the world's Gossypium genetic resources. Multinational communication and collaboration are essential to protect, secure, and evaluate the global cotton germplasm resources. Without global, collaborative efforts, the rarest and most unique cotton germplasm resources are vulnerable to extinction. C1 [Campbell, B. T.; Park, W.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Saha, S.; Jenkins, J. N.] USDA ARS, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Percy, R.; Frelichowski, J.] USDA ARS, Crop Germplasm Res Unit, College Stn, TX 77845 USA. [Mayee, C. D.; Gotmare, V.] Cent Inst Cotton Res, Nagpur 440010, Maharashtra, India. [Dessauw, D.; Giband, M.] CIRAD, F-34398 Montpellier 5, France. [Du, X.; Jia, Y.] Cotton Res Inst CAAS, Anyang 455000, Henan, Peoples R China. [Constable, G.] CSIRO Plant Ind, Narrabri, NSW 2390, Australia. [Dillon, S.] Australian Trop Grains Germplasm Ctr, Biloela, QM 4715, Australia. [Abdurakhmonov, I. Y.; Abdukarimov, A.; Rizaeva, S. M.; Adullaev, A.] Acad Sci Uzbek, Ctr Genom Technol, Inst Genet & Plant Expt Biol, Tashkent 111226, Uzbekistan. [Barroso, P. A. V.; Hoffmann, L. V.] Brazilian Agr Res Corp, Embrapa Cotton, Osvaldo Cruz, Campina Grande, PB, Brazil. [Padua, J. G.] Embrapa Cenargen, BR-70770917 Brasilia, DF, Brazil. [Podolnaya, L.] VIR, St Petersburg 190000, Russia. RP Campbell, BT (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. RI Constable, Greg/F-1069-2011; Padua, Juliano/F-3017-2012; Hoffmann, Lucia/C-1161-2013; Abdurakhmonov, Ibrokhim/F-5588-2015; Barroso, Paulo/N-7889-2015 OI Hoffmann, Lucia/0000-0003-2150-1990; Abdurakhmonov, Ibrokhim/0000-0001-9563-0686; Barroso, Paulo/0000-0003-1275-1365 NR 80 TC 34 Z9 37 U1 2 U2 14 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1161 EP 1179 DI 10.2135/cropsci2009.09.0551 PG 19 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200007 ER PT J AU Chao, SM Xu, SS Elias, EM Faris, JD Sorrells, ME AF Chao, Shiaoman Xu, Steven S. Elias, Elias M. Faris, Justin D. Sorrells, Mark E. TI Identification of Chromosome Locations of Genes Affecting Preharvest Sprouting and Seed Dormancy Using Chromosome Substitution Lines in Tetraploid Wheat (Triticum turgidum L.) SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCUS; MATURITY ALPHA-AMYLASE; GRAIN PROTEIN; AESTIVUM L.; VAR.-DICOCCOIDES; LANGDON DURUM; MAPPING GENES; SPRING WHEAT; MAJOR QTL; RESISTANCE AB Seed dormancy, the main factor contributing to preharvest sprouting (PHS) resistance, is a complex trait and is strongly influenced by environmental growth conditions. In this study, three sets of single chromosome substitution lines, including 36 genotypes, in a durum wheat [Triticum turgidum L. ssp. durum (Desf.) Husn.] background with donor chromosomes originating from three wild emmer [T turgidum L. ssp. dicoccoides (Korn. ex Asch. and Graebn.) Thell.] accessions were grown in nine field environments and evaluated for seed dormancy and PHS resistance. The substitution lines involving chromosome 3A were among the most dormant genotypes. Germination tests indicated that five chromosomes contained genes influencing seed dormancy at a level comparable to 3A. Results from PHS tests showed that PHS was affected by at least eight T dicoccoides chromosomes including 3A. The chromosomes harboring genes for seed dormancy did not fully correspond with those for PHS resistance. The weak correlations between PHS and dormancy observed in this study indicate that different genes are affecting these traits and they may I be differentially influenced by the environment. Nonetheless, our results revealed that genes present on five chromosomes, 2A, 2B, 3A, 4A, and 78, were found to affect both PHS resistance and seed dormancy. These genotypes thus provide useful resources for further studies on genetic interactions that contribute to the overall phenotypic variation and on genetic dissection of quantitative trait loci underlying PHS resistance C1 [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Xu, Steven S.; Faris, Justin D.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Elias, Elias M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Sorrells, Mark E.] Cornell Univ, Dept Genet & Plant Breeding, Ithaca, NY 14853 USA. RP Chao, SM (reprint author), USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58105 USA. FU U S Department of Agriculture-Agriculture Research Service [5442-22000-030-00D]; USDA Cooperative State Research, Education, and Extension Service [2005-35301-15728, 2006-55606-16629]; Hatch project [149419] FX The authors thank Stan Stancyk for field assistance at Prosper, ND, and Chengen Chu for help in statistical analysis This research was supported by the U S Department of Agriculture-Agriculture Research Service Current Research Information System project 5442-22000-030-00D (SC, SS, JF). This research was also supported by National Research Initiative Competitive Grants 2005-35301-15728 and 2006-55606-16629 from the USDA Cooperative State Research, Education, and Extension Service and Hatch project 149419 (MS) Shiaoman Chao would like to dedicate this work to Mike Gale, the most generous mentor, who passed away on 18 July 2009 at age 64. NR 55 TC 8 Z9 10 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1180 EP 1187 DI 10.2135/cropsci2009.10.0589 PG 8 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200008 ER PT J AU Hodnett, GL Hale, AL Packer, DJ Stelly, DM da Silva, J Rooney, WL AF Hodnett, George L. Hale, Anna L. Packer, Dan J. Stelly, David M. da Silva, Jorge Rooney, William L. TI Elimination of a Reproductive Barrier Facilitates Intergeneric Hybridization of Sorghum bicolor and Saccharum SO CROP SCIENCE LA English DT Article ID SUGARCANE; HYBRIDS; ANDROPOGONEAE; GROWTH; TISSUE; MAIZE AB Sorghum [Sorghum bicolor (L.) Moench] and sugarcane (Saccharum spp.) are now being bred to enhance the quantity and quality of biomass while maintaining or improving biotic and abiotic stress tolerances. Introgression of complementary traits between sorghum and sugarcane may be an important tool for breeders as they work toward these objectives. We show that by using a sorghum line with the lap mutant, intergeneric Sorghum bicolor x Saccharum spp. hybrids can be readily produced. Using Tx3361 as sorghum seed parent, 14,141 seed were produced from 252 sorghum x Saccharum spp. crosses. Seed set frequency in the intergeneric crosses was affected by sugarcane pollinators. The best pollinators had an above average pollen rating and favorable genetic and/or genomic compatibility, implying that breeding and selection of sugarcane pollen parents could further enhance successful hybridization. Avoiding vivipary through premature seed harvest and eliminating a physical barrier by removing the seed coat increased seedling recovery rates from 1.5 to 33%. Using these methods, our programs have grown 1371 sorghum saccharum intergeneric hybrid plants. Sufficient quantities of seedlings were produced to impose selection criteria with the goal of developing a new intergeneric cultivar with potential to be used for sugar or as a biomass feedstock. C1 [Hodnett, George L.; Packer, Dan J.; Stelly, David M.; Rooney, William L.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Hale, Anna L.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [da Silva, Jorge] Texas AgriLife Res & Extens Ctr, Weslaco, TX 78596 USA. RP Hodnett, GL (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. OI Stelly, David/0000-0002-3468-4119 NR 23 TC 9 Z9 9 U1 0 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1188 EP 1195 DI 10.2135/cropsci2009.09.0486 PG 8 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200009 ER PT J AU Mikel, MA Diers, BW Nelson, RL Smith, HH AF Mikel, Mark A. Diers, Brian W. Nelson, Randall L. Smith, Hebron H. TI Genetic Diversity and Agronomic Improvement of North American Soybean Germplasm SO CROP SCIENCE LA English DT Article ID DENT CORN; CULTIVARS; PARENTAGE; LINES; COEFFICIENTS; BARLEY; BASE AB From 1970 to 2008 there were 2242 soybean [Glycine max (L.) Merr.] cultivars registered in North America through U.S. Plant Variety Protection (PVP), U.S. utility patent, and journal registration. Of these, 80% were developed through proprietary and 20% through public programs. Our objective was to characterize the development and genetic diversity of North American soybean cultivars. The most frequently used germplasm for cultivar development were the cultivars Williams (parent used in last cross before inbreeding in 70 cultivars), A3127 (63), Essex (45), Amsoy (38), Corsoy (33), Wayne (30), Forrest (27), Hutcheson (25), MO13404 (23), and Bedford (23) Genetic diversity (1 - coefficient of parentage), estimated from pedigree lineage, was 0.89 overall. Genetic diversity was the same within public (0.89) and proprietary (0.89) cultivars. The cultivar A3127 is a major progenitor of recently developed proprietary cultivars registered from 1999 to 2008. Of these 494 cultivars, 23% have a genetic contribution of at least 25% from A3127. New cultivars were predominantly developed from the following crosses: two-parent (70% of cultivars developed), complex (12%), three-parent (5%), one backcross (5%), multiple (two, three, or four) backcrosses (3%), and five or greater backcrosses (2%). In comparisons where both parent and progeny were evaluated together, seed yield increased 3.2% per breeding cycle. In these comparisons, seed yield had a correlation of 0.29 with parental diversity. C1 [Mikel, Mark A.; Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Soybean Maize Germplasm Pathol & Genet Res Unit, USDA ARS, Urbana, IL 61801 USA. [Mikel, Mark A.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. [Mikel, Mark A.] Maize Lineage LLC, Champaign, IL 61822 USA. [Smith, Hebron H.] Brandy Wine Seed Farms LLC, Guthrie, KY 42234 USA. RP Mikel, MA (reprint author), Univ Illinois, Dept Crop Sci, Soybean Maize Germplasm Pathol & Genet Res Unit, USDA ARS, 2608 Inst Genom Biol,1206 W Gregory Dr, Urbana, IL 61801 USA. FU Roy J. Carver Biotechnology Center of the University of Illinois at Urbana-Champaign FX We thank Harry Stine for assistance with pedigree information on Midwest Oilseeds cultivars. Appreciation is extended to Paul Zankowski and Jeffrey Strachan of the Plant Variety Protection Office for providing Internet public access to the issued soybean PVP certificates We are grateful to the Roy J. Carver Biotechnology Center of the University of Illinois at Urbana-Champaign for financing the publication of this research NR 32 TC 31 Z9 34 U1 0 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1219 EP 1229 DI 10.2135/cropsci2009.08.0456 PG 11 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200012 ER PT J AU Iwata, H Jannink, JL AF Iwata, Hiroyoshi Jannink, Jean-Luc TI Marker Genotype Imputation in a Low-Marker-Density Panel with a High-Marker-Density Reference Panel: Accuracy Evaluation in Barley Breeding Lines SO CROP SCIENCE LA English DT Article ID GENOME-WIDE ASSOCIATION; LINKAGE DISEQUILIBRIUM; TRAITS; POPULATIONS; INFERENCE; SELECTION; DART AB We evaluated a strategy in which the scores of markers untyped in a low-density experimental panel were imputed on the basis of data from a high-density reference panel, in its application to whole-genome genotyping of barley (Hordeum vulgare L.) breeding lines. Using a barley core set consisting of 98 lines genotyped with 3205 markers (high-density reference panel), we imputed marker scores untyped in 863 barley breeding lines genotyped with 1330 common markers (low-density experimental panel). In repeated analyses, the scores of one common marker were masked in the experimental panel, and then imputed as an untyped marker. Imputation accuracy was evaluated by comparing imputed scores with true ones. The correct imputation rate was >0.9 in 92% of markers. The square of correlation coefficient between true genotypes and mean imputed genotypes was >0.6 in 90% of the markers. Factors affecting imputation accuracy were minor allele frequency, linkage disequilibrium with neighbor common markers, minimum distance to the closest common marker, and degree of differentiation among subpopulations. Actual quantitative trait loci (QTL) would be unobserved in both reference and experimental panels. Markers masked in both panels to mimic this situation sometimes showed larger correlation to imputed markers than to typed common markers, indicating that imputation can sometimes capture the variation of unknown QTL better than the genotypes of common markers. C1 [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Iwata, Hiroyoshi] Natl Agr & Food Res Org, Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan. RP Iwata, H (reprint author), Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan. FU USDA-CSREES [2006-55606-16722] FX This research was supported by USDA-CSREES Grant No. 2006-55606-16722, "Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Bleeding for Gene Discovery and Barley Improvement." NR 31 TC 13 Z9 13 U1 1 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1269 EP 1278 DI 10.2135/cropsci2009.08.0434 PG 10 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200018 ER PT J AU Pinson, SRM Shahjahan, AKM Rush, MC Groth, DE AF Pinson, Shannon R. M. Shahjahan, Abul K. M. Rush, M. Charles Groth, Donald E. TI Bacterial Panicle Blight Resistance QTLs in Rice and Their Association with Other Disease Resistance Loci and Heading Date SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCI; SOUTHERN UNITED-STATES; ORYZA-SATIVA L; BURKHOLDERIA-GLUMAE; FIELD-RESISTANCE; RHIZOCTONIA-SOLANI; BLAST RESISTANCE; GENES; IDENTIFICATION; PATHOGEN AB Bacterial panicle blight (BPB) of rice (Oryza sativa L.) occurs when the bacterium Burkholderia glumae Kurita and Tabei infects emerging and flowering panicles, causing kernels to abort. To identify quantitative trait loci (QTLs) for BPB resistance, a population of 300 recombinant inbred lines (RILs) derived from a cross between 'Lemont' and 'TeQing' were evaluated in 2001 and 2002 in field plots spray-inoculated with B. glumae at the time of flowering. Because this RIL population had been previously used to map QTLs for three other diseases, present use of this population allowed direct comparison between the various disease resistance QTLs. Multiple interval mapping using QTL Cartographer v2.5 putatively identified 12 BPB QTLs, three of which were statistically significant in both years and found to have epistatic effects in 2002. TeQing was the source of resistance for eight QTLs; Lemont for four. Four BPB QTLs colocated with QTLs previously identified as providing resistance to one or multiple other diseases. Three BPB QTLs were also associated with late flowering. Because late flowering panicles are subjected to cooler temperatures that are less conducive to disease development during grain fill, it is possible that the genetic effects of the heading-related QTLs were biased. The present data could not distinguish between pleiotropy and close linkage of the BPB QTLs with the previously identified heading and disease resistance QTLs. C1 [Pinson, Shannon R. M.] USDA ARS, Rice Res Unit, Beaumont, TX 77713 USA. [Shahjahan, Abul K. M.] Baton Rouge Community Coll, Baton Rouge, LA 70806 USA. [Rush, M. Charles] LSU AgCtr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Groth, Donald E.] LSU AgCtr, Rice Res Ctr, Rayne, LA 70578 USA. RP Pinson, SRM (reprint author), USDA ARS, Rice Res Unit, 1509 Aggie Dr, Beaumont, TX 77713 USA. NR 39 TC 11 Z9 13 U1 2 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1287 EP 1297 DI 10.2135/cropsci2008.07.0447 PG 11 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200020 ER PT J AU Gu, XY Zhang, L Glover, KD Chu, C Xu, SS Faris, JD Friesen, TL Ibrahim, A AF Gu, X. -Y. Zhang, L. Glover, K. D. Chu, C. Xu, S. S. Faris, J. D. Friesen, T. L. Ibrahim, A. TI Genetic Variation of Seed Dormancy in Synthetic Hexaploid Wheat-Derived Populations SO CROP SCIENCE LA English DT Article ID PREHARVEST SPROUTING TOLERANCE; WHITE WINTER-WHEAT; GRAIN DORMANCY; RESISTANCE; QTLS; INHERITANCE; GERMINATION; INHIBITORS; DAMAGE AB Aegilops tauschii, the D genome donor of wheat (Triticum aestivum L.), has strong seed dormancy and genes controlling the trait may be used to improve resistance of cultivars to pre-harvest sprouting (PHS). Thus, this research project was initiated to identify dormancy genes from Ae. tauschii-derived synthetic hexaploid wheat (SHW) Four permanent segregating populations, developed by crossing four SHW lines with a common wheat line, were grown under field conditions to evaluate dormancy with threshed seeds (kernels) and/or intact seeds on the spike from 2006 to 2008. Significant genotypic and year effects on both kernel and on-spike seed dormancy were detected in each population, with heritability estimates of about 0.4 (0.32-0.48) across the years. Seeds on the spikes were much more dormant than kernels, suggesting the presence of germination inhibitors in the covering tissues. Significant correlations between kernel and on-spike seed germination percentages were detected in all populations; the estimated correlation coefficient (r) was greater for genotypic (0 80) than for phenotypic (0.63) or environmental (0.51) components. Thus, available SHW lines could be an alternative source of dormancy genes for enhancing resistance of wheat cultivars to PHS. C1 [Gu, X. -Y.; Zhang, L.; Glover, K. D.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Chu, C.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Xu, S. S.; Faris, J. D.; Friesen, T. L.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Ibrahim, A.] Texas A&M Univ, Dept Plant & Soil Sci, College Stn, TX 77843 USA. RP Gu, XY (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. FU South Dakota Wheat Commission; U S Department of Agriculture Cooperative State Research, Education, and Extension Service [SA0900051] FX We thank B Carsrud. S Ken, and S Kalsbeck for technical assistance and two anonymous reviewers and the associate editor for their valuable comments Funding for this research was provided by South Dakota Wheat Commission and in part by U S Department of Agriculture Cooperative State Research, Education, and Extension Service (SA0900051) NR 22 TC 7 Z9 7 U1 1 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1318 EP 1324 DI 10.2135/cropsci2009.11.0645 PG 7 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200023 ER PT J AU Yu, GT Williams, CE Harris, MO Cai, XW Mergoum, M Xu, SS AF Yu, Guo Tai Williams, Christie E. Harris, Marion O. Cai, Xiwen Mergoum, Mohamed Xu, Steven S. TI Development and Validation of Molecular Markers Closely Linked to H32 for Resistance to Hessian Fly in Wheat SO CROP SCIENCE LA English DT Article ID GENE CONFERRING RESISTANCE; TRITICUM-AESTIVUM L.; BREAD WHEAT; MAYETIOLA-DESTRUCTOR; AEGILOPS-TAUSCHII; WINTER-WHEAT; SPRING WHEAT; D-GENOME; REGISTRATION; CECIDOMYIIDAE AB Hessian fly [Mayetiola destructor (Say)] is an important insect pest of wheat (Triticum aestivum L.) Host resistance conferred by H genes has been the most effective means to manage Hessian fly populations. More than 32 H genes have been identified in wheat and its relatives. In a previous study, Hessian fly-resistance gene H32 was assigned to the chromosomal bin 3DL3-0.81-1.00, which also harbors H26 The objectives of this study were to develop and validate sequence-tagged site (STS) markers closely linked to H32 and to determine the genetic relationship between H26 and H32. In this study, 11 wheat EST-derived STS markers linked to H26 and three new STS markers were added to the linkage map of H32 using the International Triticeae Mapping Initiative (ITMI) population. Two of the STS markers, Xrwgs10 and Xrwgs12, were found to flank H32 with a genetic distance of 0.5 cM. Another STS marker Xrwgs11, co-segregated with H32 Molecular markers tightly linked to H32 were validated in 12 bread wheat cultivars and an elite breeding line, demonstrating the efficacy of these markers for marker-assisted selection. Comparative mapping analysis indicated that H26 and H32 are either different alleles at the same gene locus or two different, but tightly linked H genes. Ongoing efforts to perform fine mapping and positional cloning of H26 will resolve the relationship between H26 and H32. C1 [Xu, Steven S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Yu, Guo Tai; Harris, Marion O.] N Dakota State Univ, Dept Entomol, Fargo, ND 58108 USA. [Williams, Christie E.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Cai, Xiwen; Mergoum, Mohamed] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Xu, SS (reprint author), USDA ARS, No Crop Sci Lab, 1307 18th St N, Fargo, ND 58105 USA. RI Mergoum, Mohamed/D-3815-2014 FU USDA-ARS [5442-22000-033-00D] FX This material is based on work supported by the USDA-ARS CRIS Project no 5442-22000-033-00D) NR 44 TC 7 Z9 8 U1 0 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1325 EP 1332 DI 10.2135/cropsci2009.10.0580 PG 8 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200024 ER PT J AU Yu, GT Zhang, QJ Klindworth, DL Friesen, TL Knox, R Jin, Y Zhong, SB Cai, XW Xu, SS AF Yu, Guo Tai Zhang, Qijun Klindworth, Daryl L. Friesen, Timothy L. Knox, Ron Jin, Yue Zhong, Shaobin Cai, Xiwen Xu, Steven S. TI Molecular and Cytogenetic Characterization of Wheat Introgression Lines Carrying the Stem Rust Resistance Gene Sr39 SO CROP SCIENCE LA English DT Article ID F-SP TRITICI; CONFERRING RESISTANCE; HESSIAN FLY; RACE TTKS; TRANSLOCATIONS; IDENTIFICATION; SATURATION; VIRULENCE; REGION AB The stem rust (Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn.) resistance gene Sr39, which confers resistance to TTKSK (Ug99), has been incorporated into the wheat (Triticum aestivum L.) genome from Aegilops speltoides in the form of a chromosome translocation but it has not been deployed into adapted cultivars. In this study, we characterized translocation lines carrying Sr39 in four different wheat backgrounds with fluorescent genomic in situ hybridization (GISH) and simple sequence repeat (SSR) markers. The results indicated that RL5711 and RL6082 had translocation chromosomes of comparable structure. The translocation chromosome in PI 600683 has lost Ae speltoides chromatin in the telomeric end of the 2S long arm. Six translocation lines derived from the cross PI 600683/3*HY438 had translocation chromosomes of comparable structure to the one found in PI 600683. However, one line (P9714-AM03C51), showed a substantial reduction in Ae. speltoides chromatin in the short arm of the translocated chromosome. The study demonstrated that it is apparently feasible to shorten Ae. speltoides chromatin in some wheat-Ae. speltoides translocation lines. These results and the identification of diagnostic SSR markers will be useful in guiding chromosome manipulation efforts to further shorten the Ae. speltordes chromosome segments in these materials. Greenhouse inoculation of translocation lines with stem rust indicated that the Sr39 gene conditions resistance to at least seven stem rust races. C1 [Klindworth, Daryl L.; Friesen, Timothy L.; Xu, Steven S.] ARS, USDA, Cereal Crops Res Unit, State Univ Stn, Fargo, ND 58105 USA. [Yu, Guo Tai] N Dakota State Univ, Dept Entomol, Fargo, ND 58105 USA. [Zhang, Qijun; Cai, Xiwen] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Knox, Ron] AAFC SPARC, Swift Current, SK S9H 3X2, Canada. [Jin, Yue] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Zhong, Shaobin] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. RP Klindworth, DL (reprint author), ARS, USDA, Cereal Crops Res Unit, State Univ Stn, Box 5677, Fargo, ND 58105 USA. FU Bill & Melinda Gates Foundation; USDA-ARS [5442-22000-033-00D] FX We thank Drs Justin D Fans and Lill Qi for critically reviewing the manuscript This material is supported in part by funds provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project no 5442-22000-033-00D NR 24 TC 6 Z9 8 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1393 EP 1400 DI 10.2135/cropsci2009.10.0633 PG 8 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200032 ER PT J AU Tew, TL Pan, YB AF Tew, Thomas L. Pan, Yong-Bao TI Microsatellite (Simple Sequence Repeat) Marker-based Paternity Analysis of a Seven-Parent Sugarcane Polycross SO CROP SCIENCE LA English DT Article ID CLONAL SEED ORCHARD; SWEET-POTATO; GENE FLOW; REGISTRATION; SORGHUM AB It is very challenging to make all cross combinations among elite parents used in sugarcane (Saccharum spp.) breeding programs. Hence, the polycross approach has been used to maximize the number of cross combinations that can be represented among progeny. The primary objection to using the polycross approach is the rapid loss of paternity information. Simple sequence repeat (SSR) marker-based paternity analysis is proposed as an effective molecular tool for identifying paternity of progeny from a seven-parent polycross. Using seven highly polymorphic SSR markers, 87 progeny from each polycross family were genotyped along with the parents, producing a total of 51 polymorphic SSR alleles, 15 of which were parent-specific. These alleles enabled the positive identification of the paternity for 79 to 99% of the progeny from the polycross, depending on the maternal parent The extent to which each parent contributed pollen, and thus was represented as a paternal parent, ranged from 4 to 27% Winter survival and visual ratings of the polycross progeny in the field were adversely affected by the extent of selfing within each polycross The ability to identify paternity of polycross progeny with microsatellite markers can be used in sugarcane breeding to maximize the number of desirable crosses from a limited source of flowers with minimal loss of pedigree information. C1 [Tew, Thomas L.; Pan, Yong-Bao] ARS, USDA, Sugarcane Res Lab, Houma, LA 70360 USA. RP Tew, TL (reprint author), ARS, USDA, Sugarcane Res Lab, 5883 USDA Rd, Houma, LA 70360 USA. NR 27 TC 10 Z9 11 U1 0 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1401 EP 1408 DI 10.2135/cropsci2009.10.0579 PG 8 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200033 ER PT J AU Bunce, JA AF Bunce, James A. TI Leaf Transpiration Efficiency of Some Drought-Resistant Maize Lines SO CROP SCIENCE LA English DT Article ID WATER-USE EFFICIENCY; FIELD CONDITIONS; GRAIN-SORGHUM; CORN HYBRIDS; GAS-EXCHANGE; YIELD; IMPROVEMENT; MATURITY AB Field measurements of leaf gas exchange in maize (Zea mays L.) often indicate stomatal conductances higher than required to provide substomatal carbon dioxide concentrations saturating to photosynthesis. Thus maize leaves often operate at lower transpiration efficiency (TE) than potentially achievable for species with C, metabolism. I tested whether several maize lines described as drought resistant or drought tolerant operated with higher leaf TE than less drought-adapted lines. Field measurements of leaf TE were conducted over two seasons for five reputedly drought-resistant lines of maize and three locally grown lines not regarded as drought resistant. Midday photosynthesis, transpiration, stomatal conductance, and substomatal carbon dioxide concentration measurements were made under the ambient field conditions of air temperature and humidity in Beltsville, MD Soil moisture was not limiting. Consistent and significant differences among the lines occurred for stomatal conductance, substomatal carbon dioxide concentration, and TE, but no significant differences among the lines occurred in photosynthesis. One drought-tolerant line had higher TE than all others tested, and one of the local lines had the lowest TE, but the drought-tolerant lines as a group did not have significantly higher TE. It is concluded that significant genotypic variation in leaf TE exists in maize, and that TE could be improved without reducing photosynthesis. C1 ARS, Crop Syst & Global Change Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Bunce, JA (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. NR 20 TC 11 Z9 11 U1 2 U2 30 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1409 EP 1413 DI 10.2135/cropsci2009.11.0650 PG 5 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200034 ER PT J AU Hughes, KR Griffey, CA Parrish, DJ Barbeau, WE Souza, E Thomason, WE AF Hughes, K. R. Griffey, C. A. Parrish, D. J. Barbeau, W. E. Souza, E. Thomason, W. E. TI Preharvest Sprouting Tolerance in Current Soft Red Winter Wheat Cultivars SO CROP SCIENCE LA English DT Article ID SEED COAT COLOR; HIGH-TEMPERATURE; DORMANCY; GRAIN; TRITICUM; RESISTANCE; DROUGHT; BARLEY; CROSS AB Preharvest sprouting (PHS) results in unacceptable flour quality for a portion of the mid-Atlantic soft red winter wheat (SRWW) (Triticum aestivum L) crop annually. This research evaluates inherent dormancy in current mid-Atlantic wheat cultivars using grain falling number after delayed harvest and the weighted germination index (WGI) to predict PHS. Individual spikes from 15 wheat cultivars grown in Virginia in 2007 and 2008 were harvested at physiological maturity and seeds germinated under controlled conditions. A WGI was calculated that gave greater weight to cultivars with seeds that germinated more quickly. The WGI effectively identified very high and low dormancy SRWW cultivars; however, the influence of environment during grain fill was important, especially for cultivars with moderate dormancy. To evaluate PHS tolerance by line under natural conditions, plots were left standing up to 6 wk after normal harvest date. Final harvest falling numbers were used to separate cultivars into PHS resistance groups. Strong relationships were found among PHS resistance groups, and WGI was determined at both 10 and 30 degrees C. This indicates that PHS resistance groups were good predictors of dormancy and relative response of cultivars to delayed harvest. The morphological characteristics of head angle, glume tenacity, and awn length had no significant effect on PHS. Artificial wetting resulted in a major loss of quality after just one event, emphasizing the need for timely wheat harvest. C1 [Hughes, K. R.; Griffey, C. A.; Parrish, D. J.; Thomason, W. E.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Barbeau, W. E.] Virginia Tech, Dep Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. [Souza, E.] ARS, USDA, Soft Wheat Qual Lab, Wooster, OH 44691 USA. RP Thomason, WE (reprint author), Virginia Tech, Dept Crop & Soil Environm Sci, 330 Smyth Hall, Blacksburg, VA 24061 USA. FU Maryland Gram Producers Utilization Board; Virginia Small Grams Board FX This material is based on work partially supported by the Maryland Gram Producers Utilization Board and the Virginia Small Grams Board NR 29 TC 4 Z9 4 U1 1 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1449 EP 1457 DI 10.2135/cropsci2009.09.0497 PG 9 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200039 ER PT J AU Rehman, M Hansen, JL Mallory-Smith, CA Hang, A Burton, C Zemetra, RS AF Rehman, M. Hansen, J. L. Mallory-Smith, C. A. Hang, An Burton, C. Zemetra, R. S. TI Determining the Pollen Parent of Field-Grown Backcross Progenies of Wheat (Triticum aestivum L.) x Jointed Goatgrass (Aegilops cylindrica Host) Hybrids Using Genomic In Situ Hybridization (GISH) SO CROP SCIENCE LA English DT Article ID WINTER-WHEAT; RESISTANCE GENE; INTROGRESSION; RESTITUTION; DIVISION AB Jointed goatgrass (Aegilops cylindrica Host) with CCDD genomes is a noxious weed of winter wheat (Triticum aestivum L.) Wheat (BBAADD) and jointed goatgrass (JGG) can hybridize under natural conditions, leading to a possibility of gene flow between these two species. For a gene to move from herbicide-resistant wheat to JGG, JGG must be the recurrent parent in a series (two) of backcrosses. One way of determining the pollen parent in the first backcross is to visualize C-genome chromosomes using genomic in situ hybridization (GISH). By using GISH it was determined that 7 of 88 (8%) BC(1) plants had more than seven C-genome chromosomes, indicating that JGG was the pollen parent. By using wheat-specific chloroplast simple sequence repeat marker (WCt-3) analysis, 13.6% of the hybrids had wheat as the female parent, while the remaining plants had JGG as the female parent. This study presents a reliable way of determining the pollen parent of BC(1) progeny and the hybridization dynamics between wheat and jointed goatgrass under natural field conditions This information can be used to more accurately predict the risk for gene migration between wheat and jointed goatgrass. C1 [Rehman, M.; Hansen, J. L.; Zemetra, R. S.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Mallory-Smith, C. A.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Hang, An; Burton, C.] ARS, USDA, Natl Small Grains Germplasm Res Facil, Aberdeen, ID 83210 USA. RP Rehman, M (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. NR 29 TC 2 Z9 2 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1474 EP 1479 DI 10.2135/cropsci2009.09.0509 PG 6 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200042 ER PT J AU Griess, JK Mason, SC Jackson, DS Galusha, TD Yaseen, M Pedersen, JF AF Griess, Jom K. Mason, Stephen C. Jackson, David S. Galusha, Tonne D. Yaseen, Muhammad Pedersen, Jeffrey F. TI Environment and Hybrid Influences on Food-Grade Sorghum Grain Yield and Hardness SO CROP SCIENCE LA English DT Article ID KERNEL BREAKAGE; QUALITY FACTORS; MAIZE; NITROGEN; COMPONENTS; EFFICIENCY; DENSITY AB Few studies have examined grain quality of food-grade sorghum hybrids. The objective of this study was to determine the effects of environment and hybrid on grain quality of commercially available food-grade sorghums. A randomized complete block experiment with three replications was planted in 12 environments, which included the 2004 and 2005 growing seasons and irrigated and dryland water regimes in eastern, central, and west central Nebraska and a dryland low-N environment in eastern Nebraska. Environment accounted for 5 to 140 times greater variation in measured parameters than hybrid, and the hybrid x environment interaction accounted for less than 2% of the total variation. Grain yield and kernel mass varied, with low yields of 1 4 Mg ha(-1) and kernels weighing 9.5 g 1000 kernels(-1) in the low-N 2004 environment, high grain yields of 10 5 Mg ha(-1) under irrigated conditions in central Nebraska in 2005, and kernels weighing 27 8g 1000 kernels(-1) in the eastern Nebraska dryland 2005 environment Harder grain was produced in 2005 than in 2004, with the west central and central 2005 environments having the lowest tangential abrasive dehulling device (TADD) removals of 14%. Non-food-grade hybrids produced higher grain yields and kernel mass than food-grade hybrids Grain hardness was greater for non-food-grade and medium maturity hybrids when environmental means were lower (i.e., softer) but showed little or no difference in hardness when environmental means were high. Nebraska production environments have the capability to produce high quality food-grade sorghums for specific food uses to benefit both the producer and the food processor C1 [Griess, Jom K.; Mason, Stephen C.; Galusha, Tonne D.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Jackson, David S.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. [Yaseen, Muhammad] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [Pedersen, Jeffrey F.] ARS, USDA, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Mason, SC (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RI Yaseen, Muhammad/B-4349-2012 NR 46 TC 7 Z9 7 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1480 EP 1489 DI 10.2135/cropsci2009.08.0463 PG 10 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200043 ER PT J AU Zhao, D MacKown, CT Starks, PJ Kindiger, BK AF Zhao, Duli MacKown, Charles T. Starks, Patrick J. Kindiger, Bryan K. TI Rapid Analysis of Nonstructural Carbohydrate Components in Grass Forage Using Microplate Enzymatic Assays SO CROP SCIENCE LA English DT Article ID TOMATO AB Measurements of nonstructural carbohydrates (NSC) in plant tissues are important to estimate plant organ resources available for plant growth and stress tolerance or for feed value to grazing animals. A popular commercially available assay kit used to detect glucose with a light-sensitive dye reaction was recently discontinued and replaced by a test-tube-scale glucose kit (GAHK-20) that assays glucose through enzymatic coupled reactions and the formation of reduced nicotinamide adenine dinucleotide. The objective of this study was to develop a microplate assay method that uses the GAHK-20 to quantify forage NSC composition. A laboratory microplate enzymatic method was developed for the new glucose kit and evaluated for rapidly assaying NSC components, including glucose, fructose, sucrose, fructan, and starch in 11 species of cool-season perennial grasses. By standard addition, dilution, and temporal tests of enzyme reactions, we found that this microplate enzymatic assay is a rapid and reliable method to quantify NSC composition in grass forage samples. The microplate method allows analysis of many samples per day and considerably improved time and reagent use efficiencies, especially for a large number of samples. In addition to forage, this method should be suitable for measuring NSC concentrations in fresh or dry tissues of a variety of other plant samples. C1 [Zhao, Duli] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. [MacKown, Charles T.; Starks, Patrick J.; Kindiger, Bryan K.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Zhao, D (reprint author), ARS, USDA, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. OI Kindiger, Bryan/0000-0002-2938-3653 NR 13 TC 25 Z9 27 U1 4 U2 23 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1537 EP 1545 DI 10.2135/cropsci2009.09.0521 PG 9 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200049 ER PT J AU Sanderson, MA van der Grinten, M Stout, RC AF Sanderson, Matt A. van der Grinten, Martin Stout, Robert C. TI Virginia Wildrye Persistence and Performance in Riparian Areas SO CROP SCIENCE LA English DT Article ID NORTHEAST USA AB Virginia wildrye (Elymus virginicus L.), a perennial cool-season grass native to the northeastern United States, grows in moist low ground and could be useful in revegetating riparian areas. In this field study, we compared six populations of Virginia wildrye originally collected from Maryland and New York with a commercial ecotype and a cultivar ('Omaha') on wet soils at three locations. All entries were transplanted into single-row field plots of 15 plants per plot at Wye, MD, Klingerstown, PA, and Big Flats, NY, in April 2004. Rows were oriented perpendicular to the slope so that each population was evaluated at the top, middle, and bottom slope positions. Survivorship, tillers per plant, and plant dry weight were assessed in 2007. Survivorship after 3 yr ranged from 57 to 76% for the six populations and averaged 85% for the ecotype and 77% for the cultivar. Tillers per plant ranged from 26 to 52 for the populations compared with 32 for the cultivar and 29 for the ecotype Landscape position affected wildrye plants only at the Maryland site where frequently saturated soils at the bottom slope position reduced survivorship by 50%, tiller number by 60%, and plant dry weight by 82% compared with plants at the top of the slope Most of the wild populations of Virginia wildrye were similar to the commercial cultivar and ecotype in survival, number of tillers per plant, and biomass indicating that locally adapted native populations can be used directly in conservation plantings. C1 [Sanderson, Matt A.; Stout, Robert C.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [van der Grinten, Martin] USDA NRCS, Plant Mat Ctr, Big Flats, NY 14830 USA. RP Sanderson, MA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16802 USA. NR 9 TC 0 Z9 0 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2010 VL 50 IS 4 BP 1546 EP 1551 DI 10.2135/cropsci2009.09.0542 PG 6 WC Agronomy SC Agriculture GA 615MA UT WOS:000279138200050 ER PT J AU Hernlem, B Hua, SS AF Hernlem, Bradley Hua, Sui-Sheng TI Dual Fluorochrome Flow Cytometric Assessment of Yeast Viability SO CURRENT MICROBIOLOGY LA English DT Article ID MEMBRANE; FERMENTATION; VITALITY; OXONOL; DAMAGE; CELLS; DYE AB A novel staining protocol is reported for the assessment of viability in yeast, specifically the biocontrol yeast, Pichia anomala. Employing both the red fluorescent membrane potential sensitive oxonol stain DiBAC(4)(5) (Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol), a structural analog of the commonly used DiBAC(4)(3) (Bis-(1,3-dibutylbarbituric acid)trimethine oxonol), with one of the esterase dependent green fluorogenic probes such as CFDA-AM (5-Carboxyfluorescein diacetate, acetoxymethyl ester) or Calcein-AM (Calcein acetoxymethyl ester), a two-color flow cytometric method was developed, which yields rapid quantitative information on the vitality and vigor of yeast cell cultures. The method was validated by cell sorting and analysis of live, heat killed, and UV-treated yeast. C1 [Hernlem, Bradley] ARS, Western Reg Res Ctr, Foodborne Contaminants Res Unit, USDA, Albany, CA 94710 USA. [Hua, Sui-Sheng] ARS, Western Reg Res Ctr, Plant Mycotoxins Res Unit, USDA, Albany, CA 94710 USA. RP Hernlem, B (reprint author), ARS, Western Reg Res Ctr, Foodborne Contaminants Res Unit, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM bradley.hernlem@ars.usda.gov NR 20 TC 8 Z9 8 U1 0 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 J9 CURR MICROBIOL JI Curr. Microbiol. PD JUL PY 2010 VL 61 IS 1 BP 57 EP 63 DI 10.1007/s00284-009-9576-7 PG 7 WC Microbiology SC Microbiology GA 606DI UT WOS:000278401500010 PM 20049598 ER PT J AU Flores-Mendoza, L Sotelo-Mundo, RR Dawson, H Mwangi, W Hernandez, J AF Flores-Mendoza, Lilian Sotelo-Mundo, Rogerio R. Dawson, Harry Mwangi, Waithaka Hernandez, Jesus TI Characterization of porcine CD205 SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE CD205; C-type lectin receptors; Swine ID MONOCLONAL-ANTIBODY NLDC-145; HUMAN DENDRITIC CELLS; IN-VIVO; TISSUE DISTRIBUTION; DEC-205 RECEPTOR; MANNOSE RECEPTOR; LANGERHANS CELLS; EXPRESSION; PROTEIN; ANTIGENS AB Dendritic cells express a type cell-surface receptor, CD205 that plays a role in antigen capture and delivery to the endocytic pathway. Besides DCs, high CD205 expression is also detected on thymic epithelial cells but B cells, macrophages, and T cells have limited or no expression. CD205 has been characterized in several animal species except swine. The aim of this work was to characterize porcine CD205 and mRNA expression on different cells and tissues involved in immune responses. A complete porcine CD205 sequence of 5175 bp was obtained from porcine thymus cDNA by PCR gene-walking strategy and this gene encoded a protein of 1723 amino acids. The multi-domain structure reported for murine, human, and bovine CD205 was also conserved in porcine with an overall amino acid identity of 74, 81, and 85%, respectively. Quantitative RT-PCR analysis of the CD205 mRNA profiles in normal porcine tissues and cells showed that thymus and Langerhans cells expressed the highest levels. Further characterization of porcine CD205 will lead to better understanding of the role of this receptor and development of contemporary strategies for antigen targeting to DCs in swine. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Flores-Mendoza, Lilian; Sotelo-Mundo, Rogerio R.; Hernandez, Jesus] Ctr Invest Alimentac Desarrollo AC, Lab Inmunol, Hermosillo 83000, Sonora, Mexico. [Dawson, Harry] USDA, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Mwangi, Waithaka] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA. RP Hernandez, J (reprint author), Ctr Invest Alimentac Desarrollo AC, Lab Inmunol, Carretera Victorias Km 0-6, Hermosillo 83000, Sonora, Mexico. EM jhdez@ciad.mx RI Sotelo-Mundo, Rogerio/A-6097-2011; Dawson, Harry/H-8242-2013; Hernandez, Jesus/G-2984-2012 OI Sotelo-Mundo, Rogerio/0000-0001-5543-6889; Hernandez, Jesus/0000-0002-5131-3600 FU SEP-CONACYT [82850]; TAMU-CONACYT FX This work was supported by SEP-CONACYT project No. 82850 and TAMU-CONACYT program. NR 34 TC 7 Z9 7 U1 2 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD JUL PY 2010 VL 34 IS 7 BP 715 EP 721 DI 10.1016/j.dci.2010.02.010 PG 7 WC Immunology; Zoology SC Immunology; Zoology GA 599DX UT WOS:000277891600004 PM 20188754 ER PT J AU O'Rourke, ME Sappington, TW Fleischer, SJ AF O'Rourke, Megan E. Sappington, Thomas W. Fleischer, Shelby J. TI Managing resistance to Bt crops in a genetically variable insect herbivore, Ostrinia nubilalis SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bacillus thuringensis; Bt corn; Bt cotton; European corn borer, ECB; genetically modified organism; high-dose/refuge strategy; insect-resistance management, IRM; integrated pest management, IPM; Ostrinia nubilalis; pheromone races; stable carbon isotopes ID EUROPEAN CORN-BORER; HOST-PLANT; HELICOVERPA-ZEA; SEX-PHEROMONE; GENE FLOW; LEPIDOPTERA; RACES; POPULATIONS; PYRALIDAE; EVOLUTION AB To slow the resistance evolution of the European corn borer (ECB) to Cry proteins expressed in transgenic Bacillus thuringensis (Bt) corn, the United States Environmental Protection Agency (EPA) has adopted an insect resistance management (IRM) plan that relies on a "high dose/refuge" strategy. However, this IRM plan does not consider possible ecological differences between the two ECB pheromone races (E and Z). Using carbon isotope analysis, we found that unstructured (non-corn) refuges contribute more to E race (18%) than to Z race (4%) populations of ECB in upstate New York (USA). Furthermore, feeding on non-corn hosts is associated with decreased body mass and reduced fecundity. We also show that the geographic range of E-race ECB is restricted within the range of the Z race and that E-race ECB are increasingly dominant in regions with increasing non-corn habitat. While the proportion of E-race ECB developing in unstructured refuges is higher than previously assumed, low rates of unstructured refuge use by the Z race, evidence for reduced fecundity when reared on non-corn hosts, and complete sympatry within the E race range all argue against a relaxation of current IRM refuge standards in corn based on alternative-host use. We also discuss implications of this research for integrated pest management in vegetables and IRM in Bt cotton. C1 [O'Rourke, Megan E.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Genet Lab, Ames, IA 50011 USA. [Fleischer, Shelby J.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. RP O'Rourke, ME (reprint author), Cornell Univ, Dept Ecol & Evolutionary Biol, Corson Hall,E149, Ithaca, NY 14853 USA. EM megorust@yahoo.com FU NSF; Cornell University Center for the Environment FX We thank all the Extension Educators who collected ECB samples and who contributed to the Pest Watch database. We thank Randy Ritland for technical assistance with the fecundity tests and Jean Dyer for ECB laboratory rearing. We are grateful to Sergine Ponsard for many helpful comments regarding C-isotope analyses and to Alison Power for her helpful comments on the manuscript. We also thank two anonymous reviewers for their insights. Support for this project was provided in part by NSF IGERT and EPA STAR fellowships and a Cornell University Center for the Environment grant (to M. E. O'Rourke). NR 50 TC 10 Z9 11 U1 1 U2 20 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2010 VL 20 IS 5 BP 1228 EP 1236 DI 10.1890/09-0067.1 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 614HW UT WOS:000279047400004 PM 20666246 ER PT J AU Isaak, DJ Luce, CH Rieman, BE Nagel, DE Peterson, EE Horan, DL Parkes, S Chandler, GL AF Isaak, Daniel J. Luce, Charles H. Rieman, Bruce E. Nagel, David E. Peterson, Erin E. Horan, Dona L. Parkes, Sharon Chandler, Gwynne L. TI Effects of climate change and wildfire on stream temperatures and salmonid thermal habitat in a mountain river network SO ECOLOGICAL APPLICATIONS LA English DT Article DE Boise River basin, Idaho, USA; bull trout; climate change; global warming; Oncorhynchus mykiss; patch; rainbow trout; Salvelinus confluentus; spatial statistical model; stream temperature; thermal habitat; wildfire ID WESTERN UNITED-STATES; SPATIAL STATISTICAL-MODELS; MOVING-AVERAGE APPROACH; LOWER KLAMATH RIVER; BULL TROUT; WATER TEMPERATURE; CHINOOK SALMON; PACIFIC-NORTHWEST; COLUMBIA RIVER; BROOK TROUT AB Mountain streams provide important habitats for many species, but their faunas are especially vulnerable to climate change because of ectothermic physiologies and movements that are constrained to linear networks that are easily fragmented. Effectively conserving biodiversity in these systems requires accurate downscaling of climatic trends to local habitat conditions, but downscaling is difficult in complex terrains given diverse microclimates and mediation of stream heat budgets by local conditions. We compiled a stream temperature database (n = 780) for a 2500-km river network in central Idaho to assess possible trends in summer temperatures and thermal habitat for two native salmonid species from 1993 to 2006. New spatial statistical models that account for network topology were parameterized with these data and explained 93% and 86% of the variation in mean stream temperatures and maximas, respectively. During our study period, basin average mean stream temperatures increased by 0.38 degrees C (0.27 degrees C/decade), and maximas increased by 0.48 degrees C (0.34 degrees C/decade), primarily due to long-term (30-50 year) trends in air temperatures and stream flows. Radiation increases from wildfires accounted for 9% of basin-scale temperature increases, despite burning 14% of the basin. Within wildfire perimeters, however, stream temperature increases were 2-3 times greater than basin averages, and radiation gains accounted for 50% of warming. Thermal habitat for rainbow trout (Oncorhynchus mykiss) was minimally affected by temperature increases, except for small shifts towards higher elevations. Bull trout (Salvelinus confluentus), in contrast, were estimated to have lost 11-20% (8-16%/decade) of the headwater stream lengths that were cold enough for spawning and early juvenile rearing, with the largest losses occurring in the coldest habitats. Our results suggest that a warming climate has begun to affect thermal conditions in streams and that impacts to biota will be specific to both species and context. Where species are at risk, conservation actions should be guided based on considerations of restoration opportunity and future climatic effects. To refine predictions based on thermal effects, more work is needed to understand mechanisms associated with biological responses, climate effects on other habitat features, and habitat configurations that confer population resilience. C1 [Isaak, Daniel J.; Luce, Charles H.; Rieman, Bruce E.; Nagel, David E.; Horan, Dona L.; Parkes, Sharon; Chandler, Gwynne L.] US Forest Serv, Rocky Mt Res Stn, Boise Aquat Sci Lab, Boise, ID 83702 USA. [Peterson, Erin E.] CSIRO, Div Math & Informat Sci, Indooroopilly, Qld, Australia. RP Isaak, DJ (reprint author), US Forest Serv, Rocky Mt Res Stn, Boise Aquat Sci Lab, 322 E Front St,Suite 401, Boise, ID 83702 USA. EM disaak@fs.fed.us RI Peterson, Erin/D-2360-2009; Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 FU National Fire Plan; U.S. Forest Service Rocky Mountain Research Station FX Funding for this work was provided by the National Fire Plan, the U.S. Forest Service Rocky Mountain Research Station, and local assistance from the Boise National Forest. We thank Kevin Jones, Dylan Kovis, Iosefa Matagi, and Ray Schofield for their assistance in the field. The U. S. Forest Service Remote Sensing Applications Center provided assistance with TM image processing. Stream temperature data were provided by Dan Kinney, Pacfish/Infish Biological Opinion Monitoring Group, Environmental Protection Agency, University of Idaho, and CH2M HILL, consulting. NR 150 TC 133 Z9 137 U1 17 U2 131 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2010 VL 20 IS 5 BP 1350 EP 1371 DI 10.1890/09-0822.1 PG 22 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 614HW UT WOS:000279047400012 PM 20666254 ER PT J AU Walter, WD Leslie, DM Hellgren, EC Engle, DM AF Walter, W. David Leslie, David M., Jr. Hellgren, Eric C. Engle, David M. TI Identification of subpopulations of North American elk (Cervus elaphus L.) using multiple lines of evidence: habitat use, dietary choice, and fecal stable isotopes SO ECOLOGICAL RESEARCH LA English DT Article DE Carbon isotopes; Cervus elaphus; Compositional analysis; Fecal nitrogen; Nitrogen isotopes; North American elk; Nutritional indices; Subpopulation structuring ID WHITE-TAILED DEER; ROCKY-MOUNTAIN ELK; MAMMALIAN HERBIVORES; RESOURCE SELECTION; TROPHIC ECOLOGY; MULE DEER; NITROGEN; CARBON; QUALITY; MOVEMENTS AB We used multiple lines of evidence to assess habitat selection, dietary choice, and nutritional outcomes for a population of North American elk (Cervus elaphus), confined to a relatively small and isolated landscape of public and private land in south-central Great Plains, USA. The area of suitable elk habitat was a topographically diverse matrix of mature oak savannah, C(4)-dominated grasslands, and C(3)-dominated agricultural fields surrounded by unsuitable lowlands fragmented by anthropogenic activities. We hypothesized that such disparity in habitat availability and quality resulted in subpopulation differences in the overall elk population. We used 3 methods to evaluate this premise: radiotelemetry to determine home range and habitat use, microhistology of plant fragments in feces to determine dietary selection, and fecal nitrogen (N) and stable isotope ratios of nitrogen (delta(15)N) and carbon (delta(13)C) to assess nutritional outcomes of habitat use and dietary choice. By comparing these 3 approaches, we wanted to determine if fecal indices alone could efficiently and accurately identify subpopulation structuring. Compositional analyses from radiotelemetry observations of 21 female elk identified 2 subpopulations that occupied relatively disjunct areas and showed differential preferences for forested and cultivated fields in summer but comparable preferences for cultivated fields in winter. A third unmarked subpopulation of elk was known to be largely confined to an adjacent wildlife refuge. Microhistological analyses of feces collected in all 3 areas highlighted distinct diets, outcomes of habitat occupation by the 3 subpopulations. Increased use of cultivated forages in winter was evident for 2 of the subpopulations, but the extent of use by elk was dependent on availability of cultivated forages in areas they occupied. The refuge subpopulation had no access to cultivated forage. Fecal N, fecal delta(13)C, and fecal delta(15)N supported the premise that the subpopulation with the greatest access to cultivated forages was on a higher nutritional plane than the other 2 subpopulations. Changes in fecal N, fecal delta(13)C, and fecal delta(15)N paralleled percentages of cultivated forages in the diets highlighting the utility of such fecal indices as supplemental to or surrogates for traditional methods of habitat use and dietary selection in free-ranging ungulates. C1 [Walter, W. David] Oklahoma State Univ, Dept Zool, Oklahoma Cooperat Fish & Wildlife Res Unit, Stillwater, OK 74078 USA. [Leslie, David M., Jr.] Oklahoma State Univ, US Geol Survey, Oklahoma Cooperat Fish & Wildlife Res Unit, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. [Hellgren, Eric C.] So Illinois Univ, Dept Zool, Cooperat Wildlife Res Lab, Carbondale, IL 62901 USA. [Engle, David M.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. RP Walter, WD (reprint author), Natl Wildlife Res Ctr, APHIS USDA WS, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM WDavid.Walter@colostate.edu OI Hellgren, Eric/0000-0002-3870-472X FU Rocky Mountain Elk Foundation [W-148-R] FX We thank 2 anonymous reviewers and S. D. Fuhlendorf for helpful reviews of this manuscript and R. E. Webb and D. A. Barrett for field assistance. Funding for this research was provided by the Federal Aid, Pittman-Robertson Wildlife Restoration Act under Project W-148-R of the Oklahoma Department of Wildlife Conservation and Oklahoma State University with additional contributions from the Rocky Mountain Elk Foundation, Nature Works, and BancFirst. The project was administered through the Oklahoma Cooperative Fish and Wildlife Research Unit (Oklahoma Department of Wildlife Conservation, Oklahoma State University, United States Geological Survey, United States Fish and Wildlife Service, and Wildlife Management Institute Cooperating). The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the opinions or policies of the US Government. Mention of trade, product, industry, or firm names or products or software or models, whether commercially available or not, does not constitute their endorsement by the US Government, US Fish and Wildlife Service, or US Geological Survey. NR 85 TC 4 Z9 5 U1 6 U2 44 PU SPRINGER TOKYO PI TOKYO PA 1-11-11 KUDAN-KITA, CHIYODA-KU, TOKYO, 102-0073, JAPAN SN 0912-3814 J9 ECOL RES JI Ecol. Res. PD JUL PY 2010 VL 25 IS 4 BP 789 EP 800 DI 10.1007/s11284-010-0709-z PG 12 WC Ecology SC Environmental Sciences & Ecology GA 622RL UT WOS:000279683000010 ER PT J AU Benoit, JB Morton, PK Cambron, SE Patrick, KR Schemerhorn, BJ AF Benoit, Joshua B. Morton, Philip K. Cambron, Susan E. Patrick, Kevin R. Schemerhorn, Brandon J. TI Aestivation and diapause syndromes reduce the water balance requirements for pupae of the Hessian fly, Mayetiola destructor SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE Diptera; Cecidomyiidae; dormancy; water loss; critical transition temperature; CTT; dehydration tolerance; net transpiration rate; NTR ID CUTICULAR PERMEABILITY; FLESH FLY; PHENOTYPIC PLASTICITY; GEOGRAPHIC-VARIATION; INSECT DIAPAUSE; UNITED-STATES; DIPTERA; CECIDOMYIIDAE; DESICCATION; TEMPERATURE AB We report the water balance of aestivating (summer), diapausing (winter), and non-diapausing pupae of Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae). Maintaining water requirements during pupal dormancy is particularly important because water cannot be replenished actively by drinking. Dehydration tolerance (25% loss before succumbing to dehydration) and water content (63-65%) were not different for the three types of pupae. Differences were noted in the net transpiration rates (NTRs, % body water per hour at 0% r.h.) between dormant (0.24-0.28% per hour) and non-diapausing (0.47% per hour) pupae 10 days after pupariation, but not between aestivating (0.28% per hour) and diapausing (0.24% per hour) pupae. These reduced NTRs result in extended pupal survival, indicated by adult eclosion, during exposure to dehydrating conditions. Net transpiration rates for aestivating and diapausing pupae were further reduced as dormancy progressed (up to 130 days) until individuals were moved to conditions that break dormancy. Pupae could not take up water from the atmosphere below vapor saturation (100% r.h. or 1.00 a(v)), and rely upon contact with liquid water or moist plant tissue to replenish their water stores. The critical transition temperatures (CTT) of the aestivating and diapausing pupae were significantly higher than those of non-diapausing pupae, suggesting that modified cuticular lipids are present on aestivating and diapausing pupae. Thus, aestivation and diapause trigger a dormancy specific water balance profile characterized by reduced NTRs and increased CTTs. C1 [Benoit, Joshua B.] Yale Univ, Sch Publ Hlth, Div Epidemiol Microbial Dis, New Haven, CT 06520 USA. [Benoit, Joshua B.; Patrick, Kevin R.] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. [Morton, Philip K.; Schemerhorn, Brandon J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Cambron, Susan E.; Schemerhorn, Brandon J.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Unit, W Lafayette, IN 47907 USA. RP Benoit, JB (reprint author), Yale Univ, Sch Publ Hlth, Div Epidemiol Microbial Dis, New Haven, CT 06520 USA. EM joshua.benoit@yale.edu OI Schemerhorn, Brandon/0000-0003-0247-478X NR 37 TC 7 Z9 7 U1 1 U2 3 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUL PY 2010 VL 136 IS 1 BP 89 EP 96 DI 10.1111/j.1570-7458.2010.01007.x PG 8 WC Entomology SC Entomology GA 609HI UT WOS:000278646300010 ER PT J AU Greenstone, MH Szendrei, Z Payton, ME Rowley, DL Coudron, TC Weber, DC AF Greenstone, Matthew H. Szendrei, Zsofia Payton, Mark E. Rowley, Daniel L. Coudron, Thomas C. Weber, Donald C. TI Choosing natural enemies for conservation biological control: use of the prey detectability half-life to rank key predators of Colorado potato beetle SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE Carabidae; Coccinellidae; DNA half-life; molecular gut-content analysis; PCR; Pentatomidae; predation; trophic links; Leptinotarsa decemlineata; Chrysomelidae; Coleoptera ID PODISUS-MACULIVENTRIS SAY; POLYMERASE-CHAIN-REACTION; GUT-CONTENT-ANALYSIS; COLEOMEGILLA-MACULATA COLEOPTERA; GENERALIST PREDATORS; ALTERNATIVE PREY; INVERTEBRATE PREDATION; MONOCLONAL-ANTIBODIES; HERBIVORE SUPPRESSION; HELICOVERPA-ARMIGERA AB Determining relative strengths of trophic links is critical for ranking predators for conservation biological control. Molecular gut-content analysis enables ranking by incidence of prey remains in the gut, but differential digestive rates bias such rankings toward predators with slower rates. This bias can be reduced by indexing each predator's half-life to that of the middle-most half-life in a predator complex. We demonstrate this with data from key species in the predator complex of Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), comprising adults and immatures of four taxonomically diverse species. These animals display order-of-magnitude variation in detectability half-life for the cytochrome oxidase I DNA sequence of a single CPB egg: from 7.0 h in larval Coleomegilla maculata (DeGeer) (Coleoptera: Coccinellidae) to 84.4 h in nymphal Perillus bioculatus (Fabricius) (Hemiptera: Pentatomidae). The raw species-specific incidence of L. decemlineata DNA in the guts of 351 field-collected predators ranged from 11 to 95%, ranking them as follows: C. maculata adults < Lebia grandis Hentz (Coleoptera: Carabidae) adults < Podisus maculiventris (Say) (Hemiptera: Pentatomidae) adults < P. maculiventris nymphs < P. bioculatus adults < P. bioculatus nymphs. Half-life adjustment reorders the rankings: C. maculata adults < P. bioculatus adults < P. bioculatus nymphs < P. maculiventris nymphs < L. grandis adults < P. maculiventris adults. These changes in status demonstrate the value of half-life-adjusted molecular gut-content data for ranking predators. This is the first study to measure prey detectability half-lives for the key arthropod predators of a major insect pest, and to use them to evaluate the relative impact of all adults and immatures in this predator complex. C1 [Greenstone, Matthew H.; Szendrei, Zsofia; Rowley, Daniel L.; Weber, Donald C.] ARS, USDA, IIBBL, Beltsville, MD 20705 USA. [Payton, Mark E.] Oklahoma State Univ, Dept Stat, Stillwater, OK 74078 USA. [Coudron, Thomas C.] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Greenstone, MH (reprint author), ARS, USDA, IIBBL, B-011A,Room214,BARC W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM matt.greenstone@ars.usda.gov FU Agricultural Research Service Headquarters FX We thank Jing Hu for assistance with PCR assays; Michael Athanas, Robert Bennett, James Smith, and Meiling Webb for predator rearing; Jeff Aldrich and Judy Hough-Gold-stein for providing key references; and Jon Lundgren and Alvin Simmons for carabid and thrips specimens. We also thank Cambridge University Press for permission to use previously published data. ZS was supported by an Agricultural Research Service Headquarters Post-doctoral Research Associateship to DCW. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 88 TC 35 Z9 38 U1 6 U2 55 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUL PY 2010 VL 136 IS 1 BP 97 EP 107 DI 10.1111/j.1570-7458.2010.01006.x PG 11 WC Entomology SC Entomology GA 609HI UT WOS:000278646300011 ER PT J AU Stone, KR Pilliod, DS Dwire, KA Rhoades, CC Wollrab, SP Young, MK AF Stone, Katharine R. Pilliod, David S. Dwire, Kathleen A. Rhoades, Charles C. Wollrab, Sherry P. Young, Michael K. TI Fuel Reduction Management Practices in Riparian Areas of the Western USA SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Wildfire; Fuel reduction; Prescribed fire; Thinning; Riparian ID UNITED-STATES; FIRE; FORESTS; STREAM; PERSPECTIVE; PRINCIPLES; LANDSCAPES; ECOSYSTEMS; WILDFIRE; OREGON AB Two decades of uncharacteristically severe wildfires have caused government and private land managers to actively reduce hazardous fuels to lessen wildfire severity in western forests, including riparian areas. Because riparian fuel treatments are a fairly new management strategy, we set out to document their frequency and extent on federal lands in the western U.S. Seventy-four USDA Forest Service Fire Management Officers (FMOs) in 11 states were interviewed to collect information on the number and characteristics of riparian fuel reduction treatments in their management district. Just under half of the FMOs surveyed (43%) indicated that they were conducting fuel reduction treatments in riparian areas. The primary management objective listed for these projects was either fuel reduction (81%) or ecological restoration and habitat improvement (41%), though multiple management goals were common (56%). Most projects were of small extent (93% < 300 acres), occurred in the wildland-urban interface (75%), and were conducted in ways to minimize negative impacts on species and habitats. The results of this survey suggest that managers are proceeding cautiously with treatments. To facilitate project planning and implementation, managers recommended early coordination with resource specialists, such as hydrologists and fish and wildlife biologists. Well-designed monitoring of the consequences of riparian fuel treatments on fuel loads, fire risk, and ecological effects is needed to provide a scientifically-defensible basis for the continued and growing implementation of these treatments. C1 [Stone, Katharine R.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59808 USA. [Pilliod, David S.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83706 USA. [Dwire, Kathleen A.; Rhoades, Charles C.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. [Wollrab, Sherry P.] US Forest Serv, Rocky Mt Res Stn, USDA, Boise, ID 83702 USA. [Young, Michael K.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59807 USA. RP Stone, KR (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, 5775 W Highway 10, Missoula, MT 59808 USA. EM krstone@fs.fed.us; dpilliod@usgs.gov; kadwire@fs.fed.us; crhoades@fs.fed.us; werabz@yahoo.com; mkyoung@fs.fed.us OI Pilliod, David/0000-0003-4207-3518 NR 48 TC 11 Z9 11 U1 0 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X J9 ENVIRON MANAGE JI Environ. Manage. PD JUL PY 2010 VL 46 IS 1 BP 91 EP 100 DI 10.1007/s00267-010-9501-7 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA 634BG UT WOS:000280553300008 PM 20499233 ER PT J AU Geiser, LH Jovan, SE Glavich, DA Porter, MK AF Geiser, Linda H. Jovan, Sarah E. Glavich, Doug A. Porter, Matthew K. TI Lichen-based critical loads for atmospheric nitrogen deposition in Western Oregon and Washington Forests, USA SO ENVIRONMENTAL POLLUTION LA English DT Article DE Air pollution; Atmospheric deposition; Lichen; Nitrogen; Critical load ID EPIPHYTIC MACROLICHEN COMMUNITIES; RAMALINA-MENZIESII TAYL; SOUTHERN-CALIFORNIA; HARDWOOD FORESTS; AIR-POLLUTANTS; UNITED-STATES; FIR FORESTS; VEGETATION; POLLUTION; EUROPE AB Critical loads (CLs) define maximum atmospheric deposition levels apparently preventative of ecosystem harm. We present first nitrogen CLs for northwestern North America's maritime forests. Using multiple linear regression, we related epiphytic-macrolichen community composition to: 1) wet deposition from the National Atmospheric Deposition Program, 2) wet, dry, and total N deposition from the Communities Multi-Scale Air Quality model, and 3) ambient particulate N from Interagency Monitoring of Protected Visual Environments (IMPROVE). Sensitive species declines of 20-40% were associated with CLs of 1-4 and 3-9 kg N ha(-1) y(-1) in wet and total deposition. CLs increased with precipitation across the landscape, presumably from dilution or leaching of depositional N. Tight linear correlation between lichen and IMPROVE data suggests a simple screening tool for CL exceedance in US Class I areas. The total N model replicated several US and European lichen CLs and may therefore be helpful in estimating other temperate-forest lichen CLs. Published by Elsevier Ltd. C1 [Geiser, Linda H.; Glavich, Doug A.] US Forest Serv, Pacific NW Reg Air Resource Management Program, Corvallis, OR 97339 USA. [Jovan, Sarah E.] US Forest Serv, Forest Inventory & Anal Program, Pacific NW Res Stn, Portland, OR 97205 USA. [Porter, Matthew K.] Washington State Univ, Lab Atmospher Res, Pullman, WA 99164 USA. RP Geiser, LH (reprint author), US Forest Serv, Pacific NW Reg Air Resource Management Program, POB 1148, Corvallis, OR 97339 USA. EM lgeiser@fs.fed.us FU US Forest Service Pacific Northwest Region Air and Forest Inventory Analysis programs; US-NPS Air Resources Division FX We thank the US Forest Service Pacific Northwest Region Air and Forest Inventory Analysis programs and the US-NPS Air Resources Division for funding; Greg Brenner for statistical advice; Joe Vaughn, Ray Drapek and Peter Neitlich for CMAQ and GIS assistance. Thanks to Tamara Blett and Elizabeth Waddell for encouraging us to pursue this topic. NR 58 TC 44 Z9 46 U1 5 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUL PY 2010 VL 158 IS 7 BP 2412 EP 2421 DI 10.1016/j.envpol.2010.04.001 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA 614MP UT WOS:000279063600009 PM 20447744 ER PT J AU Boateng, AA Mullen, CA Goldberg, NM Hicks, KB Devine, TE Lima, IM McMurtrey, JE AF Boateng, Akwasi A. Mullen, Charles A. Goldberg, Neil M. Hicks, Kevin B. Devine, Thomas E. Lima, Isabel M. McMurtrey, James E. TI Sustainable Production of Bioenergy and Biochar from the Straw of High-Biomass Soybean Lines via Fast Pyrolysis SO ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY LA English DT Article DE pyrolysis; blood; biochar; high-biomass; soybean straw; biofuels ID BIO-OIL; REGISTRATION; SOIL; QUALITY; ENERGY AB The straws of two high-biomass soybean lines developed at ARS for bioenergy were subjected to thermochemical conversion by fast pyrolysis. The objective was to evaluate the potential use of the straw Or the production of liquid fuel intermediates. The immediate goal was to demonstrate production of pyrolysis liquid that can be burned "as is" and/or potentially be upgraded to transportation grade fuel and at the same time produce biochar that can be deployed as a soil amendment. The goal supports the concept of on-farm biorefinery where food and bioenergy can be sustainably produced in a soybean farm system. The study shows that high yields of pyrolysis liquids (blood) can be efficiently produced from the soybean straws using the fluidized-bed fast pyrolysis technology. Free flowing biooils with heating values in excess of 20 MJ/kg were produced in yields of approximately 70 wt %. Accompanying this was 22 wt % mineral-rich biochar that can be returned to the soil and up to 10 wt % combustible gas with the potential to partially power the pyrolysis system. A sustainable farm system may be enhanced by the synergy between production of extra biomass in soybean cultivation, lignocellulosic biofuel production, production and use of carbon sequestering, soil-amending biochar in addition to nitrogen fixation by rhizobial bacteria. Although the findings are encouraging, actual data over several :years will be necessary to fully evaluate a potential soybean farm biorefinery system. by comprehensive life-cycle analysis. (C) 2010 American Institute of Chemical Engineers Environ Prog. 29: 175-183. 2010 C1 [Boateng, Akwasi A.; Mullen, Charles A.; Goldberg, Neil M.; Hicks, Kevin B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Devine, Thomas E.] ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD 20705 USA. [Lima, Isabel M.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70179 USA. [McMurtrey, James E.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Boateng, AA (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov NR 22 TC 27 Z9 27 U1 3 U2 32 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1944-7442 J9 ENVIRON PROG SUSTAIN JI Environ. Prog. Sustain. Energy PD JUL PY 2010 VL 29 IS 2 SI SI BP 175 EP 183 DI 10.1002/ep.10446 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Engineering, Chemical; Engineering, Industrial; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA 612IC UT WOS:000278889600004 ER PT J AU Yi, CX Ricciuto, D Li, R Wolbeck, J Xu, XY Nilsson, M Aires, L Albertson, JD Ammann, C Arain, MA de Araujo, AC Aubinet, M Aurela, M Barcza, Z Barr, A Berbigier, P Beringer, J Bernhofer, C Black, AT Bolstad, PV Bosveld, FC Broadmeadow, MSJ Buchmann, N Burns, SP Cellier, P Chen, JM Chen, JQ Ciais, P Clement, R Cook, BD Curtis, PS Dail, DB Dellwik, E Delpierre, N Desai, AR Dore, S Dragoni, D Drake, BG Dufrene, E Dunn, A Elbers, J Eugster, W Falk, M Feigenwinter, C Flanagan, LB Foken, T Frank, J Fuhrer, J Gianelle, D Goldstein, A Goulden, M Granier, A Grunwald, T Gu, L Guo, HQ Hammerle, A Han, SJ Hanan, NP Haszpra, L Heinesch, B Helfter, C Hendriks, D Hutley, LB Ibrom, A Jacobs, C Johansson, T Jongen, M Katul, G Kiely, G Klumpp, K Knohl, A Kolb, T Kutsch, WL Lafleur, P Laurila, T Leuning, R Lindroth, A Liu, HP Loubet, B Manca, G Marek, M Margolis, HA Martin, TA Massman, WJ Matamala, R Matteucci, G McCaughey, H Merbold, L Meyers, T Migliavacca, M Miglietta, F Misson, L Moelder, M Moncrieff, J Monson, RK Montagnani, L Montes-Helu, M Moors, E Moureaux, C Mukelabai, MM Munger, JW Myklebust, M Nagy, Z Noormets, A Oechel, W Oren, R Pallardy, SG Kyaw, TPU Pereira, JS Pilegaard, K Pinter, K Pio, C Pita, G Powell, TL Rambal, S Randerson, JT von Randow, C Rebmann, C Rinne, J Rossi, F Roulet, N Ryel, RJ Sagerfors, J Saigusa, N Sanz, MJ Mugnozza, GS Schmid, HP Seufert, G Siqueira, M Soussana, JF Starr, G Sutton, MA Tenhunen, J Tuba, Z Tuovinen, JP Valentini, R Vogel, CS Wang, JX Wang, SQ Wang, WG Welp, LR Wen, XF Wharton, S Wilkinson, M Williams, CA Wohlfahrt, G Yamamoto, S Yu, GR Zampedri, R Zhao, B Zhao, XQ AF Yi, Chuixiang Ricciuto, Daniel Li, Runze Wolbeck, John Xu, Xiyan Nilsson, Mats Aires, Luis Albertson, John D. Ammann, Christof Arain, M. Altaf de Araujo, Alessandro C. Aubinet, Marc Aurela, Mika Barcza, Zoltan Barr, Alan Berbigier, Paul Beringer, Jason Bernhofer, Christian Black, Andrew T. Bolstad, Paul V. Bosveld, Fred C. Broadmeadow, Mark S. J. Buchmann, Nina Burns, Sean P. Cellier, Pierre Chen, Jingming Chen, Jiquan Ciais, Philippe Clement, Robert Cook, Bruce D. Curtis, Peter S. Dail, D. Bryan Dellwik, Ebba Delpierre, Nicolas Desai, Ankur R. Dore, Sabina Dragoni, Danilo Drake, Bert G. Dufrene, Eric Dunn, Allison Elbers, Jan Eugster, Werner Falk, Matthias Feigenwinter, Christian Flanagan, Lawrence B. Foken, Thomas Frank, John Fuhrer, Juerg Gianelle, Damiano Goldstein, Allen Goulden, Mike Granier, Andre Gruenwald, Thomas Gu, Lianhong Guo, Haiqiang Hammerle, Albin Han, Shijie Hanan, Niall P. Haszpra, Laszlo Heinesch, Bernard Helfter, Carole Hendriks, Dimmie Hutley, Lindsay B. Ibrom, Andreas Jacobs, Cor Johansson, Torbjoern Jongen, Marjan Katul, Gabriel Kiely, Gerard Klumpp, Katja Knohl, Alexander Kolb, Thomas Kutsch, Werner L. Lafleur, Peter Laurila, Tuomas Leuning, Ray Lindroth, Anders Liu, Heping Loubet, Benjamin Manca, Giovanni Marek, Michal Margolis, Hank A. Martin, Timothy A. Massman, William J. Matamala, Roser Matteucci, Giorgio McCaughey, Harry Merbold, Lutz Meyers, Tilden Migliavacca, Mirco Miglietta, Franco Misson, Laurent Moelder, Meelis Moncrieff, John Monson, Russell K. Montagnani, Leonardo Montes-Helu, Mario Moors, Eddy Moureaux, Christine Mukelabai, Mukufute M. Munger, J. William Myklebust, May Nagy, Zoltan Noormets, Asko Oechel, Walter Oren, Ram Pallardy, Stephen G. Kyaw, Tha Paw U. Pereira, Joao S. Pilegaard, Kim Pinter, Krisztina Pio, Casimiro Pita, Gabriel Powell, Thomas L. Rambal, Serge Randerson, James T. von Randow, Celso Rebmann, Corinna Rinne, Janne Rossi, Federica Roulet, Nigel Ryel, Ronald J. Sagerfors, Jorgen Saigusa, Nobuko Sanz, Maria Jose Mugnozza, Giuseppe-Scarascia Schmid, Hans Peter Seufert, Guenther Siqueira, Mario Soussana, Jean-Francois Starr, Gregory Sutton, Mark A. Tenhunen, John Tuba, Zoltan Tuovinen, Juha-Pekka Valentini, Riccardo Vogel, Christoph S. Wang, Jingxin Wang, Shaoqiang Wang, Weiguo Welp, Lisa R. Wen, Xuefa Wharton, Sonia Wilkinson, Matthew Williams, Christopher A. Wohlfahrt, Georg Yamamoto, Susumu Yu, Guirui Zampedri, Roberto Zhao, Bin Zhao, Xinquan TI Climate control of terrestrial carbon exchange across biomes and continents SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE NEE; climate control; terrestrial carbon sequestration; temperature; dryness; eddy flux; biomes; photosynthesis; respiration; global carbon cycle ID EDDY-COVARIANCE MEASUREMENTS; LONG-TERM; INTERANNUAL VARIABILITY; SPATIAL VARIABILITY; SOIL RESPIRATION; DECIDUOUS FOREST; WATER-VAPOR; FLUXES; DIOXIDE; UNCERTAINTY AB Understanding the relationships between climate and carbon exchange by terrestrial ecosystems is critical to predict future levels of atmospheric carbon dioxide because of the potential accelerating effects of positive climate-carbon cycle feedbacks. However, directly observed relationships between climate and terrestrial CO2 exchange with the atmosphere across biomes and continents are lacking. Here we present data describing the relationships between net ecosystem exchange of carbon (NEE) and climate factors as measured using the eddy covariance method at 125 unique sites in various ecosystems over six continents with a total of 559 site-years. We find that NEE observed at eddy covariance sites is (1) a strong function of mean annual temperature at mid-and high-latitudes, (2) a strong function of dryness at mid-and low-latitudes, and (3) a function of both temperature and dryness around the mid-latitudinal belt (45 degrees N). The sensitivity of NEE to mean annual temperature breaks down at similar to 16 degrees C (a threshold value of mean annual temperature), above which no further increase of CO2 uptake with temperature was observed and dryness influence overrules temperature influence. C1 [Yi, Chuixiang; Wolbeck, John; Xu, Xiyan] CUNY, Sch Earth & Environm Sci, Queens Coll, New York, NY 11367 USA. [Ricciuto, Daniel; Gu, Lianhong] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Li, Runze] Penn State Univ, Dept Stat, University Pk, PA 16802 USA. [Nilsson, Mats; Sagerfors, Jorgen] Swedish Univ Agr Sci, Dept Forest Ecol, SE-90183 Umea, Sweden. [Albertson, John D.] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27708 USA. [Ammann, Christof; Fuhrer, Juerg] Fed Res Stn Agroscope Reckenholz Tanikon, CH-8046 Zurich, Switzerland. [Arain, M. Altaf] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada. [de Araujo, Alessandro C.] Inst Nacl de Pesquisas da Amazonia, BR-69060 Manaus, Amazonas, Brazil. [Aubinet, Marc; Heinesch, Bernard; Moureaux, Christine] Univ Liege, Unit Biosyst Phys, B-5030 Gembloux, Belgium. [Laurila, Tuomas; Tuovinen, Juha-Pekka] Finnish Meteorol Inst, FI-00101 Helsinki, Finland. [Barcza, Zoltan] Eotvos Lorand Univ, Dept Meteorol, H-1117 Budapest, Hungary. [Barr, Alan] Environm Canada, Div Climate Res, Saskatoon, SK S7N 3H5, Canada. [Berbigier, Paul] EPHYSE, UR1263, INRA, F-33883 Villenave Dornon, France. [Beringer, Jason] Monash Univ, Sch Geog & Environm Sci, Clayton, Vic, Australia. [Bernhofer, Christian] Tech Univ Dresden, Inst Hydrol & Meteorol, D-01737 Tharandt, Germany. [Black, Andrew T.] Univ British Columbia, Vancouver, BC V6T 1Z4, Canada. [Bolstad, Paul V.] Univ Minnesota, St Paul, MN 55108 USA. [Bosveld, Fred C.] Royal Netherlands Meteorol Inst, NL-3730 AE De Bilt, Netherlands. [Broadmeadow, Mark S. J.; Wilkinson, Matthew] Alice Holt Lodge, Forest Res, Farnham GU10 4LH, Surrey, England. [Buchmann, Nina; Eugster, Werner; Knohl, Alexander] Inst Plant Sci, ETH, Zurich, Switzerland. [Burns, Sean P.] Natl Ctr Atmospher Res, Boulder, CO 80307 USA. [Cellier, Pierre; Loubet, Benjamin] UMR INRA INA PG, Environm & Arable Crops Unit, F-78850 Thiverval Grignon, France. [Chen, Jingming] Univ Toronto, Dept Geog, Toronto, ON M5S 3G3, Canada. [Chen, Jiquan] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Ciais, Philippe] CNRS, CEA, UMR, LSCE, F-91191 Gif Sur Yvette, France. [Clement, Robert; Moncrieff, John] Univ Edinburgh, Sch Geosci, Edinburgh EH9 3JU, Midlothian, Scotland. [Cook, Bruce D.] NASAs Goddard Space Flight Ctr, Biospher Sci Branch, Greenbelt, MD 20771 USA. [Curtis, Peter S.] Ohio State Univ, Dept Ecol Evolut & Organismal Biol, Columbus, OH 43210 USA. [Dail, D. Bryan] Univ Maine, Dept Plant Soil & Environm Sci, Orono, ME 04469 USA. [Dellwik, Ebba] Tech Univ Denmark, Wind Energy Div, Riso Natl Lab Sustainable Energy, DK-4000 Roskilde, Denmark. [Delpierre, Nicolas; Dufrene, Eric] Univ Paris 11, Ecol Systemat & Evolut, F-91405 Orsay, France. [Desai, Ankur R.] Univ Wisconsin, Dept Atmospher & Ocean Sci, Madison, WI 53706 USA. [Dore, Sabina; Kolb, Thomas; Montes-Helu, Mario] No Arizona Univ, Sch Forestry No Arizona Univ, Flagstaff, AZ 86001 USA. [Dragoni, Danilo] Indiana Univ, Dept Geog, Atmospher Sci Program, Bloomington, IN 47405 USA. [Drake, Bert G.] Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA. [Dunn, Allison] Worcester State Coll, Dept Phys & Earth Sci, Worcester, MA 01602 USA. [Elbers, Jan; Jacobs, Cor] ESS CC, NL-6700 AA Wageningen, Netherlands. [Falk, Matthias] Atmospher Sci Grp, UC Davis, LAWR, Davis, CA 95616 USA. [Feigenwinter, Christian] Univ Basel, Inst Meteorol, CH-4056 Basel, Switzerland. [Flanagan, Lawrence B.] Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada. [Foken, Thomas] Univ Bayreuth, Dept Micrometeorol, D-95440 Bayreuth, Germany. [Frank, John; Massman, William J.] USDA Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Gianelle, Damiano; Zampedri, Roberto] Fdn E Mach, IASMA Res & Innovat Ctr, Environm & Nat Resources Area, I-38010 Trento, Italy. [Goldstein, Allen] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Goulden, Mike; Randerson, James T.] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92697 USA. [Granier, Andre] Ecol & Ecophysiol Forestier, INRA, UMR 1137, F-54280 Seichamps, France. [Gruenwald, Thomas] Tech Univ Dresden, Inst Hydrol & Meteorol, Dept Meteorol, D-01737 Dresden, Germany. [Guo, Haiqiang; Zhao, Bin] Fudan Univ, Inst Biodivers Sci, Minist Educ Key Lab Biodivers Sci & Ecol Engn, Shanghai 200433, Peoples R China. [Hammerle, Albin; Wohlfahrt, Georg] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Han, Shijie] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China. [Hanan, Niall P.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Haszpra, Laszlo] Hungarian Meteorol Serv, H-1675 Budapest, Hungary. [Helfter, Carole] Ctr Ecol & Hydrol Edinburgh Bush Estate Penicuik, Penicuik EH26 0QB, Midlothian, Scotland. [Hendriks, Dimmie] Vrije Univ Amsterdam, Dept Hydrol & Geo Environm Sci, NL-1081 HV Amsterdam, Netherlands. [Hutley, Lindsay B.] Charles Darwin Univ, Sch Environm & Life Sci, Darwin, NT 0909, Australia. [Ibrom, Andreas; Pilegaard, Kim] Tech Univ Denmark, Biosyst Div, Riso Natl Lab Sustainable Energy, DK-4000 Roskilde, Denmark. [Johansson, Torbjoern; Lindroth, Anders; Moelder, Meelis] Lund Univ, Geobiosphere Sci Ctr, Phys Geog & Ecosyst Anal, SE-22362 Lund, Sweden. [Jongen, Marjan; Pereira, Joao S.] Univ Tecn Lisboa, Inst Super Agron, P-1349017 Lisbon, Portugal. [Katul, Gabriel] Duke Univ, Sch Environm, Durham, NC 27708 USA. [Kiely, Gerard] Univ Coll Cork, Dept Civil & Environm Engn, Cork, Ireland. [Klumpp, Katja; Soussana, Jean-Francois] Unit Agron, INRA, F-63000 Clermont Ferrand, France. [Klumpp, Katja; Soussana, Jean-Francois] Inst Agrarrelevante Klimaforsch, Johann Heinrich Thunen Inst vTI, D-38116 Braunschweig, Germany. [Lafleur, Peter; Rebmann, Corinna] Trent Univ, Dept Geog, Peterborough, ON K9J 7B8, Canada. [Leuning, Ray; Myklebust, May] CSIRO Marine & Atmospher Res, Canberra, ACT, Australia. [Liu, Heping] Jackson State Univ, Dept Phys Atmospher Sci & Geosci, Jackson, MS 39217 USA. [Manca, Giovanni] Inst Atmospher Pollut Consiglio Nazl Ric, Rende Div, I-87036 Arcavacata Di Rende, Italy. [Marek, Michal] Inst Syst Biol & Ecol, Div Ecosyst Processes Lab Plants Ecol Physiol, Ceske Budejovice 370050, Czech Republic. [Martin, Timothy A.] Univ Florida, Gainesville, FL 32611 USA. [Matamala, Roser] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA. [Matteucci, Giorgio] Inst Agroenvironm & Forest Biol, Natl Res Council, I-00015 Monterotondo, RM, Italy. [McCaughey, Harry] Queens Univ, Dept Geog, Kingston, ON K7L 3N6, Canada. [Merbold, Lutz] Max Planck Inst Biogeochem, D-07745 Jena, Germany. [Meyers, Tilden] NOAA ATDD, Oak Ridge, TN 37831 USA. [Migliavacca, Mirco] Univ Milano Bicocca, Remote Sensing Environm Dynam Lab, DISAT, Milan, Italy. [Miglietta, Franco; Rossi, Federica] Ist Biometeorol, CNR, IBIMET, I-50145 Florence, Italy. [Misson, Laurent] CEFE, CNRS, F-34293 Montpellier 5, France. [Monson, Russell K.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Montagnani, Leonardo] Serv Forestali, Agenzia Ambiente, I-39100 Bolzano, Italy. [Montagnani, Leonardo] Free Univ Bozen Bolzano, Fac Sci & Technol, I-39100 Bolzano, Italy. [Moors, Eddy] Alterra Green World Res, NL-6700 AA Wageningen, Netherlands. [Moureaux, Christine] Univ Liege, Unit Crops Management, B-5030 Gembloux, Belgium. [Mukelabai, Mukufute M.] Zambian Meteorol Dept, Mongu, Zambia. [Munger, J. William] Harvard Univ, Div Engn & Appl Sci, Dept Earth & Planetary Sci, Cambridge, MA 02138 USA. [Nagy, Zoltan; Pinter, Krisztina; Tuba, Zoltan] Agr Univ Godollo, Inst Bot & Ecophysiol, H-2103 Godollo, Hungary. [Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Oechel, Walter] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Oren, Ram; Siqueira, Mario] Duke Univ, Nicholas Sch Environm & Earth Sci, Durham, NC 27708 USA. [Pallardy, Stephen G.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Pio, Casimiro] Univ Aveiro, Dept Environm, P-3810193 Aveiro, Portugal. [Pio, Casimiro] CESAM, P-3810193 Aveiro, Portugal. [Pita, Gabriel] Inst Super Tecn, Dept Mech Engn, P-1049001 Lisbon, Portugal. [Powell, Thomas L.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Rambal, Serge] UMR5175, CNRS, CEFE, DREAM, Montpellier 5, France. [von Randow, Celso] Natl Inst Space Res, Ctr Earth Syst Sci, BR-12630 Cachoeira Paulista, SP, Brazil. [Rinne, Janne] Univ Helsinki, Dept Phys, FIN-00014 Helsinki, Finland. [Ryel, Ronald J.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Saigusa, Nobuko] Natl Inst Environm Studies, Ctr Global Environm Res, Tsukuba, Ibaraki 3058506, Japan. [Sanz, Maria Jose] Ctr Estud Ambient Mediterraneo, E-46980 Paterna, Spain. [Mugnozza, Giuseppe-Scarascia] Dept Agron Forestry & Land Use, Agr Res Council, I-00184 Rome, Italy. [Schmid, Hans Peter] Atmospher Environm Res Inst Meteorol, Garmisch Partenkirchen, Germany. [Schmid, Hans Peter] Climate Res Forschungszentrum Karlsruhe, Garmisch Partenkirchen, Germany. [Seufert, Guenther] Inst Environm & Sustainabil, Joint Res Ctr European Commiss, I-21020 Ispra, Italy. [Starr, Gregory] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Sutton, Mark A.] Atmospher Sci Ctr Ecol & Hydrol CEH, Penicuik EH26 0QB, Midlothian, Scotland. [Tenhunen, John] Univ Bayreuth, Dept Plant Ecol, D-95440 Bayreuth, Germany. [Valentini, Riccardo] Univ Tuscia, Dept Forest Environm & Resources, I-01100 Viterbo, Italy. [Vogel, Christoph S.] Univ Michigan Biol Stn, Pellston, MI 49769 USA. [Wang, Jingxin] Liaoning Normal Univ, Sch Math, Dalian 116039, Peoples R China. [Wang, Shaoqiang; Wen, Xuefa; Yu, Guirui] Chinese Acad Sci, Inst Geog Sci & Nat Resource Res, Beijing 100101, Peoples R China. [Wang, Weiguo] NOAA, IMSG Natl Ctr Environm Predict, Camp Springs, MD 20746 USA. [Welp, Lisa R.] Univ Calif, Geosci Res Div, Scripps Inst Oceanog, La Jolla, CA 92093 USA. [Wharton, Sonia] Lawrence Livermore Natl Lab, Atmospher Earth & Energy Div, Livermore, CA 94551 USA. [Williams, Christopher A.] Clark Univ, Grad Degree Program Geog, Worcester, MA 01610 USA. [Yamamoto, Susumu] Okayama Univ, Okayama 7008530, Japan. [Zhao, Xinquan] Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Jiangxi, Peoples R China. RP Yi, CX (reprint author), CUNY, Sch Earth & Environm Sci, Queens Coll, New York, NY 11367 USA. RI Powell, Thomas/F-9877-2016; Ricciuto, Daniel/I-3659-2016; Munger, J/H-4502-2013; Hammerle, Albin/N-4345-2016; Soussana, Jean-Francois/P-2094-2016; Gianelle, Damiano/G-9437-2011; Gu, Lianhong/H-8241-2014; Goldstein, Allen/A-6857-2011; Oechel, Walter/F-9361-2010; Goulden, Michael/B-9934-2008; Eugster, Werner/E-5116-2010; Beringer, Jason/B-8528-2008; Soussana, Jean-Francois/E-2543-2012; Rinne, Janne/A-6302-2008; Chen, Jiquan/D-1955-2009; Ryel, Ronald/A-3422-2008; Burns, Sean/A-9352-2008; Miglietta, Franco/A-1257-2009; Feigenwinter, Christian/A-6151-2008; Buchmann, Nina/E-6095-2011; Noormets, Asko/A-7257-2009; Katul, Gabriel/A-7210-2008; Helfter, Carole/A-1835-2010; Feigenwinter, Christian/A-4606-2012; Valentini, Riccardo/D-1226-2010; Ibrom, Andreas/A-9850-2011; Leuning, Ray/A-2793-2008; Pio, Casimiro/A-8135-2008; Hutley, Lindsay/A-7925-2011; Zhao, Bin/E-5349-2010; Schmid, Hans Peter/I-1224-2012; Migliavacca, mirco/C-1260-2011; Moors, Eddy/J-5165-2012; Sutton, Mark/K-2700-2012; Wang, Weiguo/B-4948-2009; Flanagan, Lawrence/B-1307-2013; Elbers, Jan/D-9477-2013; von Randow, Celso/B-3335-2009; Pilegaard, Kim/I-7137-2013; Kiely, Gerard/I-8158-2013; Cook, Bruce/M-4828-2013; Yi, Chuixiang/A-1388-2013; Zhao, Bin/I-3651-2013; 于, 贵瑞/C-1768-2014; Knohl, Alexander/F-9453-2014; Barcza, Zoltan/G-3880-2014; Garmisch-Pa, Ifu/H-9902-2014; Li, Runze/C-5444-2013; Aurela, Mika/L-4724-2014; Lindroth, Anders/N-4697-2014; Wohlfahrt, Georg/D-2409-2009; Marek, Michal V./D-4383-2014; Merbold, Lutz/K-6103-2012; Desai, Ankur/A-5899-2008; Xu, Xiyan/D-2854-2015; Barr, Alan/H-9939-2014; Seufert, Gunther/J-9918-2013; Matteucci, Giorgio/N-3526-2015; Sanz Sanchez, Maria Jose/A-6099-2016; Meyers, Tilden/C-6633-2016; Montagnani, Leonardo/F-1837-2016; OI Powell, Thomas/0000-0002-3516-7164; Ricciuto, Daniel/0000-0002-3668-3021; Munger, J/0000-0002-1042-8452; Hammerle, Albin/0000-0003-1963-5906; Soussana, Jean-Francois/0000-0002-1932-6583; Gianelle, Damiano/0000-0001-7697-5793; Gu, Lianhong/0000-0001-5756-8738; Goldstein, Allen/0000-0003-4014-4896; Oechel, Walter/0000-0002-3504-026X; Eugster, Werner/0000-0001-6067-0741; Beringer, Jason/0000-0002-4619-8361; Rinne, Janne/0000-0003-1168-7138; Burns, Sean/0000-0002-6258-1838; Miglietta, Franco/0000-0003-1474-8143; Feigenwinter, Christian/0000-0003-2447-5492; Noormets, Asko/0000-0003-2221-2111; Katul, Gabriel/0000-0001-9768-3693; Valentini, Riccardo/0000-0002-6756-5634; Pio, Casimiro/0000-0002-3531-8620; Hutley, Lindsay/0000-0001-5533-9886; Schmid, Hans Peter/0000-0001-9076-4466; Moors, Eddy/0000-0003-2309-2887; Flanagan, Lawrence/0000-0003-1748-0306; Elbers, Jan/0000-0002-0631-3505; von Randow, Celso/0000-0003-1045-4316; Pilegaard, Kim/0000-0002-5169-5717; Cook, Bruce/0000-0002-8528-000X; Zhao, Bin/0000-0002-3530-2469; Knohl, Alexander/0000-0002-7615-8870; Barcza, Zoltan/0000-0002-1278-0636; Li, Runze/0000-0002-0154-2202; Lindroth, Anders/0000-0002-7669-784X; Wohlfahrt, Georg/0000-0003-3080-6702; Merbold, Lutz/0000-0003-4974-170X; Desai, Ankur/0000-0002-5226-6041; Xu, Xiyan/0000-0003-2732-1325; Seufert, Gunther/0000-0002-6019-6688; Matteucci, Giorgio/0000-0002-4790-9540; Sanz Sanchez, Maria Jose/0000-0003-0471-3094; Montagnani, Leonardo/0000-0003-2957-9071; Hanan, Niall/0000-0002-9130-5306; Goulden, Michael/0000-0002-9379-3948; Martin, Timothy/0000-0002-7872-4194; Foken, Thomas/0000-0003-4562-9083; Aires, Luis Miguel Igreja/0000-0003-2052-6045; Kiely, Gerard/0000-0003-2189-6427; Ibrom, Andreas/0000-0002-1341-921X; Jongen, Marjan/0000-0003-1026-6622; Pita, Gabriel/0000-0002-2225-5309; Arain, M. Altaf/0000-0002-1433-5173; rambal, serge/0000-0001-5869-8382; Pereira, Joao/0000-0002-3658-5587 FU National Science Foundation [NSF-DEB-0949637]; PSC-CUNY [62787-00 40]; CarboEuropeIP; FAO-GTOS-TCO; iLEAPS; Max Planck Institute for Biogeochemistry; University of Tuscia; US Department of Energy; AmeriFlux; Fluxnet-Canada; ChinaFlux; OzFlux; CarboAfrica; AsiaFlux; Department of Commerce (NOAA); Department of Agriculture (USDA/Forest Service); NASA; European Commission; Canadian Foundation for Climate and Atmospheric Sciences (CFCAS); Natural Sciences and Engineering Research Council (NSERC); BIOCAP Canada (Fluxnet-Canada only); Chinese Academy of Sciences; Ministry of Science and Technology; Australian Research Council; Ministry of Agriculture, Forest and Fisheries (MAFF); Ministry of Industrial Trade and Industry (MITI); Ministry of Education, Science, Sports and Culture (MESSC); Brazilian Ministry of Science and Technology (MCT); LBA FX This work was financially supported in part by the National Science Foundation (NSF-DEB-0949637) and the PSC-CUNY Faculty Research Award (Grant No 62787-00 40). This work was based on the database produced by the La Thuile FLUXNET project, which received financial support of CarboEuropeIP, FAO-GTOS-TCO, iLEAPS, Max Planck Institute for Biogeochemistry, National Science Foundation, University of Tuscia, US Department of Energy. We acknowledge database and technical support from Berkeley Water Center, Lawrence Berkeley National Laboratory, Microsoft Research eScience, Oak Ridge National Laboratory, University of California-Berkeley, University of Virginia. The following regional networks were involved with this work: AmeriFlux, CarboEuropeIP, Fluxnet-Canada, ChinaFlux, OzFlux, CarboAfrica, and AsiaFlux. AmeriFlux, is sponsored by the United States Departments of Energy (Terrestrial Carbon Program, National Institutes of Global Environmental Change (NIGEC), National Institute for Climate Change Research (NICCR)), Department of Commerce (NOAA), and Department of Agriculture (USDA/Forest Service), NASA, and the National Science Foundation. European sites in the NitroEurope, Euroflux and Medeflu projects are supported by the European Commission Directorate General XII Environment, Climate Program and the Program CONSOLIDER-INGENIO 2010 (GRACCIE). Canadian sites are sponsored by the Canadian Foundation for Climate and Atmospheric Sciences (CFCAS), the Natural Sciences and Engineering Research Council (NSERC), and BIOCAP Canada (Fluxnet-Canada only). Chinese sites are supported by the Chinese Academy of Sciences and the Ministry of Science and Technology. Australian sites are supported by the Australian Research Council. The Japanese site is supported by the Ministry of Agriculture, Forest and Fisheries (MAFF), the Ministry of Industrial Trade and Industry (MITI), and Ministry of Education, Science, Sports and Culture (MESSC). The Brazilian site is supported by the Brazilian Ministry of Science and Technology (MCT) and the LBA program. We thank the numerous scientists, students, and technicians responsible for the day-to-day gathering of the flux data, and the agency representatives who fund the respective projects. Without the dedicated efforts of so many individuals, this analysis would be impossible. NR 45 TC 56 Z9 58 U1 5 U2 132 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD JUL-SEP PY 2010 VL 5 IS 3 AR 034007 DI 10.1088/1748-9326/5/3/034007 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 655TX UT WOS:000282273700008 ER PT J AU Bolster, CH Cook, KL Marcus, IM Haznedaroglu, BZ Walker, SL AF Bolster, Carl H. Cook, Kimberly L. Marcus, Ian M. Haznedaroglu, Berat Z. Walker, Sharon L. TI Correlating Transport Behavior with Cell Properties for Eight Porcine Escherichia coli Isolates SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SATURATED POROUS-MEDIA; GROWTH-PHASE; IONIC-STRENGTH; COLI STRAINS; ADHESION; DIVERSITY; SURFACES; BACTERIA; SAND; LIPOPOLYSACCHARIDES AB In this study we investigate how growth stage and depositional environment affect variability of cell properties and transport behavior of eight porcine E. coli isolates. We compared the surface properties for cells harvested during exponential and stationary growth phase and their transport behavior through columns packed with either uncoated or Fe-coated quartz sand. We then investigated correlations between measured cell properties and fitted bacterial attachment efficiencies. For both growth stages we found that bacterial attachment efficiencies in the uncoated quartz sand varied among the eight different isolates by over an order of magnitude whereas attachment efficiencies in the Fe-coated sands varied by a factor of less than two. With the exception of one isolate, growth condition had minimal impact on attachment efficiencies to the uncoated sands. A strong and statistically significant inverse relationship was observed between bacterial attachment efficiencies in the uncoated quartz sand columns and log-transformed zeta potential, whereas a mild yet statistically significant relationship between bacterial attachment efficiencies in the Fe-coated sands and cell width was observed. For the experimental conditions used in our study, we found that variability in E. coli transport was more dependent on the depositional environment than on growth conditions. C1 [Bolster, Carl H.; Cook, Kimberly L.] USDA ARS, Bowling Green, KY 42104 USA. [Marcus, Ian M.; Haznedaroglu, Berat Z.; Walker, Sharon L.] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA. RP Bolster, CH (reprint author), USDA ARS, 230 Bennett Ln, Bowling Green, KY 42104 USA. EM carl.bolster@ars.usda.gov RI Haznedaroglu, Berat/A-6467-2011 OI Haznedaroglu, Berat/0000-0002-0081-8801 NR 38 TC 32 Z9 32 U1 1 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 1 PY 2010 VL 44 IS 13 BP 5008 EP 5014 DI 10.1021/es1010253 PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 617TL UT WOS:000279304700032 PM 20540542 ER PT J AU Tellinghuisen, J Bolster, CH AF Tellinghuisen, Joel Bolster, Carl H. TI Least-Squares Analysis of Phosphorus Soil Sorption Data with Weighting from Variance Function Estimation: A Statistical Case for the Freundlich Isotherm SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID LIGAND-BINDING SYSTEMS; PHOSPHATE SORPTION; REGRESSION-ANALYSIS; ENZYME-KINETICS; CALIBRATION; PARAMETERS; PRECISION; CONSTANTS; CURVES; ERROR AB Phosphorus soil sorption data are typically fitted to simple isotherms for the purpose of compactly summarizing experimental results and extrapolating beyond the range of measurements. Here, the question of which of the commonly preferred models-Langmuir and Freundlich-is better, is addressed using weighted least-squares, with weights obtained by variance function analysis of replicate data. Proper weighting in this case requires attention to a special problem-that the dependent variable S is not measured, rather is calculated from the measured equilibrium concentration C. The latter is commonly taken as the independent variable but is subjectto experimental error, violating a fundamental least-squares assumption. This problem is handled through an effective variance treatment When the data are fitted to the Langmuir, Freundlich, and Temkin isotherms, only the Freundlich model yields a statistically adequate chi(2) value, and then only when S is taken to include labile residual P (S(0)) estimated from isotope-exchange experiments. The Freundlich model also yields good estimates of S(0) when this is treated as an adjustable parameter rather than a known quantity-of relevance to studies in which S(0) is not measured. By contrast, neglect of weights and labile P can lead to a mistaken preference for the Langmuir model. C1 [Tellinghuisen, Joel] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA. [Bolster, Carl H.] USDA ARS, Bowling Green, KY 42104 USA. RP Tellinghuisen, J (reprint author), Vanderbilt Univ, Dept Chem, Box 1583, Nashville, TN 37235 USA. EM joel.tellinghuisen@vanderbilt.edu NR 49 TC 9 Z9 9 U1 2 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 1 PY 2010 VL 44 IS 13 BP 5029 EP 5034 DI 10.1021/es100535b PG 6 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 617TL UT WOS:000279304700035 PM 20540572 ER PT J AU Carles, CC Fletcher, JC AF Carles, Cristel C. Fletcher, Jennifer C. TI Missing links between histones and RNA Pol II arising from SAND? SO EPIGENETICS LA English DT Article DE gene activation; chromatin; SAND domain protein; ULT1; AIRE; trithorax group; stem cell; RNA polymerase II ID STEM-CELL MAINTENANCE; POLYCOMB-GROUP; AUTOIMMUNE REGULATOR; FLORAL MERISTEM; TRANSCRIPTION FACTOR; GENE-EXPRESSION; BINDING PROTEIN; CONTROLS SHOOT; PHD FINGER; ARABIDOPSIS AB Correct deployment of developmental programs and maintenance of cell fates in eukaryotes rely on the timely activation or repression of gene expression. These processes depend to a large extent on modifications of chromatin structure that modulate the access of transcription factors to target DNA. In particular, Polycomb group (PcG) and trithorax group (trxG) chromatin remodeling complexes play key roles in depositing repressive and active histone marks, respectively, to maintain stable expression of developmental target genes. Yet despite enormous insights into both chromatin modification and transcription, the molecular mechanisms through which these two key processes influence each other are still quite nebulous. Recent independent studies from plant and human model systems have potentially uncovered a common ground for coordinating chromatin remodeling and transcriptional events. In this review, we discuss the function of the SAND domain proteins ULTRAPETALA1 (ULT1) and Aire as molecular links between chromatin remodelers and transcription effectors. C1 [Fletcher, Jennifer C.] Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. [Carles, Cristel C.] Univ Grenoble 1, CNRS CEA UJF CEA INRA, Grenoble UMR5168, Physiol Cellulaire Vegetale Lab, Grenoble, France. [Fletcher, Jennifer C.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Fletcher, JC (reprint author), Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. EM jfletcher@berkeley.edu FU US National Science Foundation; US Department of Agriculture; CNRS FX The authors' research is supported by funding from the US National Science Foundation (J.C.F.), the US Department of Agriculture (J.C.F.) and the CNRS-Higher Education Chair program (C.C.C.). NR 42 TC 9 Z9 9 U1 1 U2 5 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1559-2294 EI 1559-2308 J9 EPIGENETICS-US JI Epigenetics PD JUL 1 PY 2010 VL 5 IS 5 BP 381 EP 385 PG 5 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 629OQ UT WOS:000280209900004 PM 20458168 ER PT J AU Zhang, LH Brown, CR Culley, D Baker, B Kunibe, E Denney, H Smith, C Ward, N Beavert, T Coburn, J Pavek, JJ Dauenhauer, N Dauenhauer, R AF Zhang, Linhai Brown, Charles R. Culley, David Baker, Barbara Kunibe, Elizabeth Denney, Hazel Smith, Cassandra Ward, Neuee Beavert, Tia Coburn, Julie Pavek, J. J. Dauenhauer, Nora Dauenhauer, Richard TI Inferred origin of several Native American potatoes from the Pacific Northwest and Southeast Alaska using SSR markers SO EUPHYTICA LA English DT Article DE Solanum tuberosum; Group Andigenum; Chilean potato; Chloroplast genome; Simple sequence repeats; Phylogenetics; Makah; Quillayute; Haida; Tlingit; Ozette; Kasaan; Maria's; To-Le-Ak ID SIMPLE SEQUENCE REPEATS; SOLANUM-TUBEROSUM; CHLOROPLAST DNA; CULTIVATED POTATO; SINGLE DOMESTICATION; EVOLUTION; MICROSATELLITES; AMPLIFICATION; POPULATIONS; DELETION AB Certain Native Americans from the Pacific Northwest and Alaska of the USA have grown potatoes in their gardens for many generations. In this study, the origin of several potatoes collected from Native gardens was investigated. Fourteen SSR markers covering the 12 potato homologs yielding a total of 199 alleles were amplified and scored in Solanum tuberosum Group Andigena (52 accessions), S. tuberosum Group. Tuberosum (39 accessions) and wild species (6 accessions). "Ozette" from the Makah Nation on the Olympic Peninsula in Washington State was closely related to "Maria's" and "Kasaan" potatoes collected from Native Alaskan gardens in Southeast Alaska. These three potatoes were more closely related to either two Mexican and one Peruvian andigena accessions or three Chilean Group Tuberosum accessions, while being relatively less related to the old European or modern varieties and most distantly related to Group Andigenum. "To-Le-Ak" was closely related to two Chilean tuberosum accessions and one old European variety. All Native potatoes harbored T-type chloroplast genome indicating that their maternal lineage is shared with Chilean Group Tuberosum. Using genetic relationship as a guide to origin it appears plausible that the Native American/Alaskan cultivars are either directly or indirectly from Mexico and Chile. These Native potato cultivars present a possible second route of diffusion distinct from the South America to Europe transfer which has been assumed to the sole means by which potato was spread out of South America. C1 [Brown, Charles R.] ARS, USDA, Prosser, WA 99350 USA. [Zhang, Linhai; Culley, David] Washington State Univ, IAREC, Prosser, WA 99350 USA. [Baker, Barbara] USDA ARS UC Berkeley, Ctr Plant Gene Express, Albany, CA 94710 USA. [Kunibe, Elizabeth; Dauenhauer, Nora; Dauenhauer, Richard] Southeast Alaska Univ, Juneau, AK USA. [Denney, Hazel; Smith, Cassandra; Ward, Neuee] Makah Nation Makah Res & Cultural Ctr, Neah Bay, WA USA. [Beavert, Tia] Yakama Nation Heritage Univ, Toppenish, WA USA. [Coburn, Julie] Haida Nation, Kasaan, AK USA. [Pavek, J. J.] ARS, USDA, Aberdeen, ID USA. RP Brown, CR (reprint author), ARS, USDA, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM chuck.brown@ars.usda.gov RI Baker, Barbara/L-7198-2016; OI Baker, Barbara/0000-0002-1276-971X; Zhang, Linhai/0000-0003-1009-6870 FU National Science Foundation; Makah (Neah Bay, Washington); Tlingit Nation; Quillayute Nation; Haida Nation FX This research is a part of the Potato Genome Project funded by National Science Foundation. We thank the Makah (Neah Bay, Washington), Tlingit, Quillayute and Haida Nations for their help and support. NR 52 TC 3 Z9 4 U1 2 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2010 VL 174 IS 1 BP 15 EP 29 DI 10.1007/s10681-009-0092-4 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 605LD UT WOS:000278348200002 ER PT J AU Moser, BR Winkler-Moser, JK Shah, SN Vaughn, SF AF Moser, Bryan R. Winkler-Moser, Jill K. Shah, Shailesh N. Vaughn, Steven F. TI Composition and physical properties of arugula, shepherd's purse, and upland cress oils SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Arugula; Barbarea verna; Capsella bursa-pastoris; Eruca vesicaria (L.) subspecies sativa; Shepherd's purse ID FATTY-ACID-COMPOSITION; ERUCA-SATIVA; BIODIESEL; LEPIDIUM; BARBAREA; ESTERS; SEED AB The fatty acid, tocopherol, and phytosterol profiles of arugula (AO; Eruca vesicaria (L.) Cav. subsp. sativa (Mill.) Thell.), upland cress (UCO; Barbarea verna (Mill.) Asch.), and shepherd's purse (SPO; Capsella bursa-pastoris (L.) Medik.) oils are reported, along with their physical properties. The tocopherol content of SPO (770 mg/kg) was higher than detected in AO (656 mg/kg) and UCO (430 mg/kg). UCO contained a higher concentration of phytosterols (8.47 mg/g) than SPO (7.19 mg/g) and AO (6.60 mg/g). The oil contents of AO, SPO, and UCO were 27.0, 26.6, and 24.2 wt%. The acid values of AO (0.54 mg KOH/g) and UCO (0.32 mg KOH/g) were lower than obtained for SPO (3.19 mg KOH/g). The principle fatty acid in AO and UCO was erucic acid (41.7 and 36.8 wt%), whereas SPO contained primarily linolenic (32.4 wt%) and linoleic (20.5 wt%) acids. AO and UCO were more stable to oxidation than SPO, as indicated by induction periods (110 C; EN 14 112) of 16.5, 7.9, and 2.2 h. SPO exhibited lower kinematic viscosity and higher viscosity index values than AO and UCO. UCO displayed the lowest pour point (-29 degrees C), followed by AO (-22 degrees C) and SPO (-14 degrees C). C1 [Moser, Bryan R.; Winkler-Moser, Jill K.; Shah, Shailesh N.; Vaughn, Steven F.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Moser, BR (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov NR 23 TC 4 Z9 4 U1 1 U2 14 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1438-7697 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD JUL PY 2010 VL 112 IS 7 BP 734 EP 740 DI 10.1002/ejlt.200900219 PG 7 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 635AT UT WOS:000280628700007 ER PT J AU Joshi, H Moser, BR Shah, SN Mandalika, A Walker, T AF Joshi, Hem Moser, Bryan R. Shah, Shailesh N. Mandalika, Anurag Walker, Terry TI Improvement of fuel properties of cottonseed oil methyl esters with commercial additives SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Biodiesel; Cottonseed; Fatty acid methyl esters; Gossypium hirsutum; Gossypol ID BIODIESEL PRODUCTION; VEGETABLE-OIL; DIESEL FUELS; FATTY-ACIDS; SOYBEAN OIL; GOSSYPOL; DERIVATIVES; STABILITY; PALM AB The low temperature operability and oxidative stability of cottonseed oil methyl esters (CSME) were improved with four anti-gel additives as well as one antioxidant additive, gossypol. Low temperature operability and oxidative stability of CSME was determined by cloud point (CP), pour point (PP), cold filter plugging point (CFPP), and oxidative stability index (OSI). The most significant reductions in CP, PP, and CFPP in all cases were obtained with Technol (R), with the average reduction in temperature found to be 3.9 degrees C. Gunk (R), Heet (R), and Howe's (R) were progressively less effective, as indicated by average reductions in temperature of 3.4, 3.0, and 2.8 degrees C, respectively. In all cases, the magnitude of CFPP reduction was greater than for PP and especially CP. Addition of gossypol, a polyphenolic aldehyde, resulted in linear improvement in OSI (R(2) = 0.9804). The OSI of CSME increased from 5.0 to 8.3 h with gossypol at a concentration of 1000 ppm. C1 [Joshi, Hem; Mandalika, Anurag; Walker, Terry] Clemson Univ, Clemson, SC 29634 USA. [Moser, Bryan R.; Shah, Shailesh N.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. RP Walker, T (reprint author), Clemson Univ, 111 Biosyst Res Complex, Clemson, SC 29634 USA. EM walker4@clemson.edu FU USDA-ARS Southwestern Cotton Ginning Research Laboratory (SWCGRL); Cotton, Inc. FX The authors acknowledge Benetria N. Banks and Erin L. Walter for excellent technical assistance. The authors also thank USDA-ARS Southwestern Cotton Ginning Research Laboratory (SWCGRL) in Cooperative 269 R&D Agreement with Eco-Sol, LLC for their support and supply of cottonseed oil. Also many thanks are extended to Cotton, Inc. for funding a portion of this project. NR 39 TC 15 Z9 15 U1 1 U2 6 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1438-7697 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD JUL PY 2010 VL 112 IS 7 BP 802 EP 809 DI 10.1002/ejlt.200900291 PG 8 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 635AT UT WOS:000280628700014 ER PT J AU Chiu, EYH Lin, YH Wu, W Song, QJ Chang, PFL Gao, LY Chou, CC Ueng, PP AF Chiu, Ericka Yen-Hsin Lin, Ying-Hong Wu, Wei Song, Qijian Chang, Pi-Fang Linda Gao, Ling-Yan Chou, Chun-Chi Ueng, Peter P. TI Alternative splicing and genetic diversity of the white collar-1 (wc-1) gene in cereal Phaeosphaeria pathogens SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Alternative splicing; Phaeosphaeria; White collar-1; Sporulation; Phylogenetic relationship ID PRE-MESSENGER-RNA; BETA-GLOBIN GENE; NEUROSPORA-CRASSA; TRANSCRIPTION; NODORUM; PROTEIN; SPORULATION; THALASSEMIA; MECHANISMS; REPRESSION AB The white collar-1 (wc-1) gene encodes an important light-responsive protein (wc-1) that maintains circadian clocks and controls numerous light-dependent reactions including sporulation in ascomycete fungi. The structure and expression of the wc-1 gene in wheat-biotype Phaeosphaeria nodorum (PN-w) was studied. It was shown that the full-size (3,353 bp in length) wc-1 gene in PN-w contained 4 introns in which introns 1 and 2 were flanked by GC-AG splice borders and were spliced constitutively. However, introns 3 and 4 of the wc-1 gene were alternatively spliced. As the result of alternative splicing (AS), six transcript variants were identified, encoding different lengths of deduced polypeptides (from 1,044 to 1,065aa). Ratios of the wc-1 gene transcript variants in the RNA population were the same in the sporulated and non-sporulated PN-w isolate Sn37-1 and the sporulated PN-w isolate S-79-1, grown under light/dark conditions. The AS of the wc-1 gene may control various light-dependent reactions in PN-w, which leads to diverse morphological, physiological and pathological characters for pathogen infection and spread. Based on the nucleotide and deduced amino acid sequences, the wc-1 gene in cereal Phaeosphaeria pathogens was diverse. It appeared that the deduced wc-1 polypeptide sequences of P. avenaria f. sp. avenaria (Paa), P. avenaria f. sp. triticea (Pat1 and Pat3) and barley biotype P. nodorum (PN-b) were more closely related than PN-w and Phaeosphaeeria sp. (P-rye) from Poland. Based on the wc-1 deduced polypeptide sequences, P. avenaria f. sp. triticea (Pat2) from foxtail barley (Hordeum jubatum L.) was evolutionary well separated from the other cereal Phaeosphaeria pathogens. C1 [Lin, Ying-Hong; Chang, Pi-Fang Linda] Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 402, Taiwan. [Chiu, Ericka Yen-Hsin] Seed Improvement & Propagat Stn, Taichung 426, Taiwan. [Wu, Wei] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian, Peoples R China. [Song, Qijian] Univ Maryland, Dept Nat Resource Sci & Landscape Architecture, College Pk, MD 20742 USA. [Gao, Ling-Yan] Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Hohhot City, Inner Mongolia, Peoples R China. [Chou, Chun-Chi] Tenha Life Sci Co, Tainan 744, Taiwan. [Ueng, Peter P.] ARS, Mol Plant Pathol Lab, Inst Plant Sci, USDA, Beltsville, MD 20705 USA. RP Chang, PFL (reprint author), Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 402, Taiwan. EM pfchang@nchu.edu.tw; ppuuueng@gmail.com FU Department of International Affairs, Council of Agriculture, Executive Yuan, Taiwan [97AS-4.1.2-IC-I1(6), 98AS-4.1.1-IC-I1(1)]; Ministry of Education, Taiwan; National Chung Hsing University, Taiwan FX The corresponding author (PPU) is indebted to Barry M. Cunfer, Emeritus Professor of the University of Georgia and Gregory E. Shaner, Emeritus Professor of Purdue University, for their career-long inspiration, guidance and collaboration on research in cereal Stagonospora diseases. We also thank Rosemarie Hammond of the USDA-ARS for reviewing the manuscript. This research was supported in part by Department of International Affairs, Council of Agriculture, Executive Yuan, Taiwan under grant numbers 97AS-4.1.2-IC-I1(6) and 98AS-4.1.1-IC-I1(1), by the Ministry of Education, Taiwan, under the ATU plan, and by National Chung Hsing University, Taiwan. NR 35 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2010 VL 127 IS 3 BP 351 EP 363 DI 10.1007/s10658-010-9602-4 PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 606DP UT WOS:000278402300006 ER PT J AU von der Ohe, C Gauthier, V Tamburic-Ilincic, L Brule-Babel, A Fernando, WGD Clear, R Ward, TJ Miedaner, T AF von der Ohe, Christiane Gauthier, Victoria Tamburic-Ilincic, Lily Brule-Babel, Anita Fernando, W. G. Dilantha Clear, Randy Ward, Todd J. Miedaner, Thomas TI A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Chemotype; DON; FHB index ID HEAD BLIGHT; GIBBERELLA-ZEAE; DON CONTENT; RESISTANCE; POPULATION; CULMORUM; GRAIN; TRICHOTHECENES; EPIDEMIOLOGY; RYE AB Twenty four isolates of Fusarium graminearum, half of which were 3-acetyldeoxynivalenol (3-ADON) and half 15-acetyldeoxynivalenol (15-ADON) chemotypes, were tested for their ability to produce deoxynivalenol and to cause Fusarium head blight (FHB) in spring wheat cultivars. The objectives of this study were to determine (1) whether 3-ADON isolates differ in aggressiveness, as measured by the FHB index, and DON production from 15-ADON isolates under field conditions, and (2) whether the performance of resistant host cultivars was stable across isolates. Field tests of all isolates were conducted with three replicates at each of two locations in Canada and Germany in 2008 with three host genotypes differing in FHB resistance level. The resistant host genotype showed resistance regardless of the chemotype or location. The differences between mean FHB indices of 3-ADON and 15-ADON isolates were not significant for any wheat genotype. In contrast, average DON production by the 3-ADON isolates (10.44 mg kg(-1)) was significantly (P < 0.05) higher than for the 15-ADON isolates (6.95 mg kg(-1)) at three of the four locations where moderately resistant lines were tested, and at both locations where susceptible lines were evaluated. These results indicate that 3-ADON isolates could pose a greater risk to food safety. However, as the mean aggressiveness and DON production of 3-ADON and 15-ADON chemotypes was similar on highly resistant lines, breeding and use of highly resistant lines is still the most effective measure of reducing the risks associated with DON in wheat. C1 [von der Ohe, Christiane; Miedaner, Thomas] Univ Stuttgart Hohenheim, State Plant Breeding Inst, D-70599 Stuttgart, Germany. [Gauthier, Victoria; Brule-Babel, Anita; Fernando, W. G. Dilantha] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada. [Tamburic-Ilincic, Lily] Univ Guelph, Dept Plant Agr, Ridgetown, ON N0P 2C0, Canada. [Clear, Randy] Canadian Grain Commiss, Grain Res Lab, Winnipeg, MB R3C 3G8, Canada. [Ward, Todd J.] ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP Miedaner, T (reprint author), Univ Stuttgart Hohenheim, State Plant Breeding Inst, D-70599 Stuttgart, Germany. EM miedaner@uni-hohenheim.de OI Miedaner, Thomas/0000-0002-9541-3726 FU Federal Ministry of Education and Research (BMBF) in Germany [0313711A]; National Science and Engineering Research Council, Husky Energy Inc.; Western Grains Research Foundation; Agri Food Research & Development Initiative; Ontario Wheat Producers Marketing Board (OWMPB); Canadian Agricultural Adaptation Council FX This research was funded by the Federal Ministry of Education and Research (BMBF) in Germany within the framework of GABI CANADA (grant no. 0313711A), the National Science and Engineering Research Council, Husky Energy Inc., Western Grains Research Foundation, Agri Food Research & Development Initiative, Ontario Wheat Producers Marketing Board (OWMPB), and the Canadian Agricultural Adaptation Council. The authors wish to thank all technicians and students who helped in conducting the field trials and disease evaluations at each location. NR 32 TC 44 Z9 45 U1 0 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2010 VL 127 IS 3 BP 407 EP 417 DI 10.1007/s10658-010-9607-z PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 606DP UT WOS:000278402300011 ER PT J AU Skoracka, A Smith, L Oldfield, G Cristofaro, M Amrine, JW AF Skoracka, Anna Smith, Lincoln Oldfield, George Cristofaro, Massimo Amrine, James W. TI Host-plant specificity and specialization in eriophyoid mites and their importance for the use of eriophyoid mites as biocontrol agents of weeds SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Biological control; Eriophyoidea; Generalists; Host ranges; Host plants; Specialists ID BIOLOGICAL-CONTROL AGENT; CEREAL RUST MITE; ABACARUS-HYSTRIX; PHYTOPHAGOUS INSECTS; HERBIVOROUS INSECTS; ACERIA-CHONDRILLAE; METRIONA-ELATIOR; SPECIES COMPLEX; SOLANUM-VIARUM; SALSOLA-TRAGUS AB Eriophyoid mites, which are among the smallest plant feeders, are characterized by the intimate relationships they have with their hosts and the restricted range of plants upon which they can reproduce. The knowledge of their true host ranges and mechanisms causing host specificity is fundamental to understanding mite-host interactions, potential mite-host coevolution, and diversity of this group, as well as to apply effective control strategies or to use them as effective biological control agents. The aim of this paper is to review current knowledge on host specificity and specialization in eriophyoid mites, and to point out knowledge gaps and doubts. Using available data on described species and recorded hosts we showed that: (1) 80% of eriophyoids have been reported on only one host species, 95% on one host genus, and 99% on one host family; (2) Diptilomiopidae has the highest proportion of monophagous species and Phytoptidae has the fewest; (3) non-monophagous eriophyoids show the tendency to infest closely related hosts; 4) vagrant eriophyoids have a higher proportion of monophagous species than refuge-seeking and refuge-inducing species; (5) the proportions of monophagous species infesting annual and perennial hosts are similar; however, many species infesting annual hosts have wider host ranges than those infesting perennial hosts; (6) the proportions of species that are monophagous infesting evergreen and deciduous plants are similar; (7) non-monophagous eriophyoid species have wider geographic distribution than monophagous species. Field and laboratory host-specificity tests for several eriophyoid species and their importance for biological control of weeds are described. Testing the actual host range of a given eriophyoid species, searching for ecological data, genetic differentiation analysis, and recognizing factors and mechanisms that contribute to host specificity of eriophyoid mites are suggested as future directions for research. C1 [Skoracka, Anna] Adam Mickiewicz Univ Poznan, Inst Environm Biol, Dept Anim Taxon & Ecol, Fac Biol, PL-61614 Poznan, Poland. [Smith, Lincoln] ARS, Exot & Invas Weeds Res Unit, USDA, Albany, CA 94710 USA. [Oldfield, George] ARS, USDA, Riverside, CA 92521 USA. [Amrine, James W.] W Virginia Univ, Morgantown, WV 26506 USA. [Cristofaro, Massimo] BAS BIOTEC SIC, BBCAonlus ENEA CR, I-00123 Rome, Italy. RP Skoracka, A (reprint author), Adam Mickiewicz Univ Poznan, Inst Environm Biol, Dept Anim Taxon & Ecol, Fac Biol, Umultowska 89, PL-61614 Poznan, Poland. EM anna.skoracka@amu.edu.pl RI Skoracka, Anna/E-6002-2011 OI Skoracka, Anna/0000-0002-9485-532X FU Polish Ministry of Science and Higher Education [NN303089434] FX The authors would like to thank Lechoslaw Kuczynski (Adam Mickiewicz University, Poznan), Enrico de Lillo (University of Bari, Italy), Brian Rector (USDA), and two anonymous referees for their valuable suggestions on the manuscript. Research on host specialization carried out by A. S was supported by the Polish Ministry of Science and Higher Education (grant no. NN303089434). NR 92 TC 38 Z9 39 U1 0 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD JUL PY 2010 VL 51 IS 1-3 SI SI BP 93 EP 113 DI 10.1007/s10493-009-9323-6 PG 21 WC Entomology SC Entomology GA 581EW UT WOS:000276505300006 PM 19789985 ER PT J AU Smith, L de Lillo, E Amrine, JW AF Smith, L. de Lillo, E. Amrine, J. W., Jr. TI Effectiveness of eriophyid mites for biological control of weedy plants and challenges for future research SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Biocontrol; Invasive plant; Weed; Host plant specificity; Efficacy ID SKELETONWEED CHONDRILLA-JUNCEA; ACERIA-ANTHOCOPTES ACARI; MALHERBAE NUZZACI ACARI; ABACARUS-HYSTRIX ACARI; CEREAL RUST MITE; LANTANA-CAMARA; CONTROL AGENT; HOST-SPECIFICITY; FIELD BINDWEED; FLORACARUS-PERREPAE AB Eriophyid mites have been considered to have a high potential for use as classical biological control agents of weeds. We reviewed known examples of the use of eriophyid mites to control weedy plants to learn how effective they have been. In the past 13 years, since Rosenthal's 1996 review, 13 species have undergone some degree of pre-release evaluation (Aceria genistae, A. lantanae, Aceria sp. [boneseed leaf buckle mite (BLBM)], A. salsolae, A. sobhiani, A. solstitialis, A. tamaricis, A. thalgi, A. thessalonicae, Cecidophyes rouhollahi, Floracarus perrepae, Leipothrix dipsacivagus and L. knautiae), but only four (A. genistae, Aceria sp. [BLBM], C. rouhollahi and F. perrepae) have been authorized for introduction. Prior to this, three species (Aceria chondrillae, A. malherbae and Aculus hyperici) were introduced and have become established. Although these three species impact the fitness of their host plant, it is not clear how much they have contributed to reduction of the population of the target weed. In some cases, natural enemies, resistant plant genotypes, and adverse abiotic conditions have reduced the ability of eriophyid mites to control target weed populations. Some eriophyid mites that are highly coevolved with their host plant may be poor prospects for biological control because of host plant resistance or tolerance of the plant to the mite. Susceptibility of eriophyids to predators and pathogens may also prevent them from achieving population densities necessary to reduce host plant populations. Short generation time, high intrinsic rate of increase and high mobility by aerial dispersal imply that eriophyids should have rapid rates of evolution. This raises concerns that eriophyids may be more likely to lose efficacy over time due to coevolution with the target weed or that they may be more likely to adapt to nontarget host plants compared to insects, which have a longer generation time and slower population growth rate. Critical areas for future research include life history, foraging and dispersal behavior, mechanisms controlling host plant specificity, and evolutionary stability of eriophyid mites. This knowledge is critical for designing and interpreting laboratory and field experiments to measure host plant specificity and potential impact on target and nontarget plants, which must be known before they can be approved for release. One of the more successful examples of an eriophyid mite controlling an invasive alien weed is Phyllocoptes fructiphilus, whose impact is primarily due to transmission of a virus pathogenic to the target, Rosa multiflora. Neither the mite nor the virus originated from the target weed, which suggests that using "novel enemies" may sometimes be an effective strategy for using eriophyid mites. C1 [Smith, L.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [de Lillo, E.] Univ Bari, Fac Agr, Dipartimento Biol & Chim Agroforestale & Ambienta, I-70126 Bari, Italy. [Amrine, J. W., Jr.] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. RP Smith, L (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM link.smith@ars.usda.gov OI de Lillo, Enrico/0000-0003-0364-4963 NR 227 TC 34 Z9 35 U1 1 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD JUL PY 2010 VL 51 IS 1-3 SI SI BP 115 EP 149 DI 10.1007/s10493-009-9299-2 PG 35 WC Entomology SC Entomology GA 581EW UT WOS:000276505300007 PM 19760101 ER PT J AU Gamliel-Atinsky, E Freeman, S Maymon, M Belausov, E Ochoa, R Bauchan, G Skoracka, A Pena, J Palevsky, E AF Gamliel-Atinsky, Efrat Freeman, Stanley Maymon, Marcel Belausov, Eduard Ochoa, Ronald Bauchan, Gary Skoracka, Anna Pena, Jorge Palevsky, Eric TI The role of eriophyoids in fungal pathogen epidemiology, mere association or true interaction? SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Eriophyoidea; Fungi; Mite-fungus-plant interactions; Aceria mangiferae; Abacarus hystrix; Fusarium mangiferae; Puccinia spp. ID MANGO MALFORMATION DISEASE; ROBINI CLAPAREDE ACARI; FUSARIUM-MANGIFERAE; CAUSAL AGENT; POPULATION-GROWTH; ACERIA-MANGIFERAE; POWDERY MILDEW; BULB MITE; SUBGLUTINANS; PREDATORS AB A considerable number of plant feeding mites representing different families such as Acaridae, Siteroptidae, Tydeidae, and Tarsonemidae interact with plant pathogenic fungi. While species within the Eriophyoidea appear to be the most common phytophagous mites vectoring virus diseases, little is known of their role in fungal pathogen epidemiology. In the present article, we present two studies on eriophyoid-fungal relationships. The first focusing on the association between Aceria mangiferae and the fungal pathogen Fusarium mangiferae in mango is presented as a case study. The second, as the research is still in a preliminary phase, reports on quantitative and descriptive associations between the cereal rust mite Abacarus hystrix and rusts caused by Puccinia spp. Mango bud tissue colonized with F. mangiferae, and wheat and quackgrass leaves colonized with Puccinia spp., supported significantly higher populations of eriophyoid mites. Both mite species were observed bearing the spores of the respective pathogens on their body integument. Aceria mangiferae vectored the pathogen's spores into the bud, the sole port of entry for the fungal pathogen and the frequency and severity of fungal infection increased in the presence of A. mangiferae. While it appears that eriophyoids are playing a role in fungal epidemiology, clearly further research is needed to enhance our understanding of direct and indirect (plant mediated) interactions between plant pathogens and eriophyoid mites in different plant-pathogen systems. C1 [Palevsky, Eric] Newe Yaar Res Ctr ARO, Dept Entomol, IL-30095 Ramat Yishay, Israel. [Pena, Jorge] Univ Florida, Ctr Trop Res & Educ, Dept Entomol & Nematol, Homestead, FL 33031 USA. [Skoracka, Anna] Adam Mickiewicz Univ Poznan, Fac Biol, Dept Anim Taxon & Ecol, Inst Environm Biol, PL-61614 Poznan, Poland. [Bauchan, Gary] ARS, Electron & Confocal Microscopy Unit, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Ochoa, Ronald] ARS, Systemat Entomol Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Belausov, Eduard] Agr Res Org, Volcani Ctr, Microscopy Unit, IL-50250 Bet Dagan, Israel. [Freeman, Stanley; Maymon, Marcel] Agr Res Org, Volcani Ctr, Dept Plant Pathol & Weed Res, IL-50250 Bet Dagan, Israel. [Gamliel-Atinsky, Efrat] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. RP Palevsky, E (reprint author), Newe Yaar Res Ctr ARO, Dept Entomol, POB 1021, IL-30095 Ramat Yishay, Israel. EM palevsky@volcani.agri.gov.il RI Al Adawi, Ali/H-4593-2011; Skoracka, Anna/E-6002-2011 OI Skoracka, Anna/0000-0002-9485-532X FU Israeli Ministry of Agriculture [132-0972]; Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development [TA-MOU-02-M21-030] FX This research was supported in part by grant no. 132-0972 from the Chief Scientist of the Israeli Ministry of Agriculture, and by the Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development, under the terms of the Middle East Regional Cooperation Program Award No. TA-MOU-02-M21-030, awarded to SF. We would like to express our gratitude to Drs. H. Voet (Hebrew University, Israel) and Lechoslaw Kuczynski (Adam Mickiewicz University, Poznan, Poland) for advice in the statistical analyses, Prof. D. Shtienberg (ARO) for advice on epidemiological studies, A. Zveibel (ARO), Y. Denisov (ARO) and M. Sharon (ARO) for technical assistance, Drs. G. Miller (SEL-ARS-USDA), Matt Buffington, G. Evans (APHIS-USDA) and Prof. Uri Gerson (Hebrew University) for their assistance in the preparation and review of the manuscript, and Magdalena Gawlak (IOR, Poznan, Poland) for taking SEM photos of A. hystrix and Puccinia sp. NR 64 TC 14 Z9 14 U1 0 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD JUL PY 2010 VL 51 IS 1-3 SI SI BP 191 EP 204 DI 10.1007/s10493-009-9302-y PG 14 WC Entomology SC Entomology GA 581EW UT WOS:000276505300010 PM 19774470 ER PT J AU Navia, D Ochoa, R Welbourn, C Ferragut, F AF Navia, Denise Ochoa, Ronald Welbourn, Cal Ferragut, Francisco TI Adventive eriophyoid mites: a global review of their impact, pathways, prevention and challenges SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Review DE Acari; Eriophyoidea; Invasive alien species; Pathways; Quarantine; Biosecurity ID STREAK-MOSAIC-VIRUS; ACERIA-GUERRERONIS ACARI; COCONUT MITE; BIOLOGICAL-CONTROL; TRISETACUS-JUNIPERINUS; HOST-RANGE; RUST MITE; WHEAT; TOSICHELLA; KEIFER AB Eriophyoids have high potential as adventive mite species (AMS) because their small size make them difficult to detect, and can be easily distributed in world trade. Economic, social and environmental impact from adventive eriophyoid mites has been significant. Considerable attention has been given to adventive insect species while adventive mites have received little attention and little information is available for eriophyoids. This paper summarizes information on adventive eriophyoid mites, their impact, and the history of some important invasions. The status of adventive species of eriophyoids introduced as biological control agents of weeds is presented. A list of eriophyoid mites reported as invasive species worldwide is given. Pathways of concern and biosecurity actions to reduce the risk of eriophyoid mites are discussed. The need to raise public awareness of the risk and importance of these tiny organisms as AMS is emphasized. Scientific and technical challenges to deal with adventive eriophyoids are discussed. C1 [Navia, Denise] Embrapa Recursos Genet & Biotecnol, Lab Quarentena Vegetal, BR-70770900 Brasilia, DF, Brazil. [Ochoa, Ronald] ARS, Systemat Entomol Lab, USDA, BARC W, Beltsville, MD 20705 USA. [Welbourn, Cal] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA. [Ferragut, Francisco] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, Valencia 46022, Spain. RP Navia, D (reprint author), Embrapa Recursos Genet & Biotecnol, Lab Quarentena Vegetal, CP 02372, BR-70770900 Brasilia, DF, Brazil. EM navia@cenargen.embrapa.br; Ron.Ochoa@ars.usda.gov; welbouc@doacs.state.fl.us; fjferrag@eaf.upv.es RI Navia, Denise/C-5016-2013 FU CNPq FX We thank Drs. Greg Evans (APHIS-USDA), Gary Miller (USDA-SEL), Jim Amrine (University of West Virginia) and Eddie Ueckermann (Agricultural Research Council, South Africa) for review and helpful comments on the manuscript. To Dr. Lincoln Smith for valuable suggestions and revision of the text related to the potential of weed control eriophyoids as adventive species. To Dr. Gary Bauchan and Mr. Eric Erbe, USDA-ECMU kindly provided for the low temperature scanning electron microscope photos of several species of Eriophyidae. To Peter Touhey and Geoff White (USDA) supplied species list data for the USA. To CNPq for the research fellowship to the first author. NR 175 TC 20 Z9 21 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD JUL PY 2010 VL 51 IS 1-3 SI SI BP 225 EP 255 DI 10.1007/s10493-009-9327-2 PG 31 WC Entomology SC Entomology GA 581EW UT WOS:000276505300012 PM 19844795 ER PT J AU Rammer, DR Zelinka, SL AF Rammer, D. R. Zelinka, S. L. TI OPTICAL METHOD FOR MEASURING THE SURFACE AREA OF A THREADED FASTENER SO EXPERIMENTAL TECHNIQUES LA English DT Article ID TREATED WOOD; CORROSION C1 [Rammer, D. R.; Zelinka, S. L.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Rammer, DR (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM drammer@fs.fed.us RI Zelinka, Samuel/A-9198-2009 NR 9 TC 3 Z9 3 U1 0 U2 5 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0732-8818 J9 EXP TECHNIQUES JI Exp. Tech. PD JUL-AUG PY 2010 VL 34 IS 4 BP 36 EP 39 DI 10.1111/j.1747-1567.2009.00538.x PG 4 WC Engineering, Mechanical; Mechanics; Materials Science, Characterization & Testing SC Engineering; Mechanics; Materials Science GA 631OY UT WOS:000280358500005 ER PT J AU Downey, LH Castellanos, DC Yadrick, K Threadgill, P Kennedy, B Strickland, E Prewitt, TE Bogle, M AF Downey, Laura H. Castellanos, Diana Cuy Yadrick, Kathy Threadgill, Paula Kennedy, Betty Strickland, Earline Prewitt, T. Elaine Bogle, Margaret TI Capacity Building for Health Through Community-Based Participatory Nutrition Intervention Research in Rural Communities SO FAMILY & COMMUNITY HEALTH LA English DT Article DE community-based participatory research; community capacity; intervention; physical activity; sustainability ID LOWER MISSISSIPPI DELTA; AFRICAN-AMERICANS; DISTRUST AB Since its inception, capacity building has been a stated goal of the Delta Nutrition Intervention Research Initiative, a tri-state collaboration in the Lower Mississippi Delta to address high rates of chronic disease. Textual analysis of project documents identifies and describes strategies carried out to foster capacity building. Strategies to build community capacity include fostering participation, cultivating leadership opportunities, training community members as co-researchers, securing community resources, and implementing the intervention together. Incorporating capacity-building approaches in health promotion and nutrition-intervention programming in rural communities provides a means to enhance potential for sustainability of health outcomes and developed effectiveness. C1 [Downey, Laura H.] Univ So Mississippi, Dept Community Hlth Sci, Hattiesburg, MS 39406 USA. [Castellanos, Diana Cuy; Yadrick, Kathy] Univ So Mississippi, Dept Nutr & Food Syst, Hattiesburg, MS 39406 USA. [Threadgill, Paula] Mississippi State Univ, Sch Human Sci, Mississippi State, MS 39762 USA. [Kennedy, Betty] Pennington Biomed Res Ctr, Baton Rouge, LA USA. [Strickland, Earline] ARS, Delta Obes Prevent Res Unit, USDA, Jackson, MS USA. [Prewitt, T. Elaine] Univ Arkansas Med Sci, Little Rock, AR 72205 USA. [Bogle, Margaret] ARS, So Plains Area, USDA, Little Rock, AR USA. RP Downey, LH (reprint author), Univ So Mississippi, Dept Community Hlth Sci, 118 Coll Dr,Box 5122, Hattiesburg, MS 39406 USA. EM laura.m.hall@usm.edu NR 29 TC 2 Z9 2 U1 2 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0160-6379 J9 FAM COMMUNITY HEALTH JI Fam. Community Health PD JUL-SEP PY 2010 VL 33 IS 3 BP 175 EP 185 DI 10.1097/FCH.0b013e3181e4bb58 PG 11 WC Family Studies; Public, Environmental & Occupational Health SC Family Studies; Public, Environmental & Occupational Health GA 607WU UT WOS:000278538800004 PM 20531098 ER PT J AU Feng, PCH Keys, C Lacher, D Monday, SR Shelton, D Rozand, C Rivas, M Whittam, T AF Feng, Peter C. H. Keys, Christine Lacher, David Monday, Steven R. Shelton, Dan Rozand, Christine Rivas, Marta Whittam, Thomas TI Prevalence, characterization and clonal analysis of Escherichia coli O157: non-H7 serotypes that carry eae alleles SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE Escherichia coli; O157:non-H7; eae alleles; clonality ID WILD BIRDS; VIRULENCE FACTORS; GENETICS ANALYSIS; MULTIPLEX PCR; STRAINS; IDENTIFICATION AB We examined O157:non-H7 strains isolated from various sources and geographical locations and found 15/57 strains to carry eae alleles, including alpha, beta, epsilon and kappa/delta, suggesting that these strains may be prevalent. All strains were serologically and genetically confirmed to be O157, but none were the H7 serotype or carried any trait virulence factors of the Escherichia coli O157:H7 serotype. Genetic H typing of the eae-positive strains showed that the alpha-eae-bearing strain was H45, while the beta- and epsilon-eae strains were H16 and the kappa/delta-eae strains were H39. The beta- and epsilon-eae-bearing O157:H16 strains shared similar to 90% pulsed-field gel electrophoresis (PFGE) similarity and were distinct from the other strains that had other eae alleles. Interestingly, an epsilon-eae O157:H16 strain isolated from meat in France shared PFGE similarity to the O157:H16 strains from water in the United States. Multilocus sequence typing showed that there is clonal diversity within the O157 serogroup, as some O157:non-H7 strains clustered with EPEC clonal groups, while others clustered within the ST-171 group of diverse strains and serotypes that had not previously included any strains from the O157 serogroup. Clonal analysis also showed that none of the eae-positive O157:non-H7 strains we examined were closely related to the pathogenic O157:H7 serotype. C1 [Feng, Peter C. H.; Keys, Christine; Monday, Steven R.] US FDA, Div Microbiol, College Pk, MD 20740 USA. [Lacher, David] US FDA, Div Mol Biol, Laurel, MD USA. [Shelton, Dan] ARS, USDA, Beltsville, MD USA. [Rozand, Christine] BioMerieux, Div Ind & Environm, Marcy I Etoile, France. [Rivas, Marta] INEI ANLIS, Serv Fisiopatogenia, Buenos Aires, DF, Argentina. [Whittam, Thomas] Michigan State Univ, Natl Food Safety & Toxicol Ctr, E Lansing, MI 48824 USA. RP Feng, PCH (reprint author), US FDA, Div Microbiol, HFS 711,5100 Paint Branch Pkwy, College Pk, MD 20740 USA. EM peter.feng@fda.hhs.gov NR 21 TC 13 Z9 13 U1 0 U2 4 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0378-1097 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD JUL PY 2010 VL 308 IS 1 BP 62 EP 67 DI 10.1111/j.1574-6968.2010.01990.x PG 6 WC Microbiology SC Microbiology GA 604WA UT WOS:000278308000010 PM 20487015 ER PT J AU Anderson, RC Flythe, MD Krueger, NA Callaway, TR Edrington, TS Harvey, RB Nisbet, DJ AF Anderson, R. C. Flythe, M. D. Krueger, N. A. Callaway, T. R. Edrington, T. S. Harvey, R. B. Nisbet, D. J. TI Decreased Competiveness of the Foodborne Pathogen Campylobacter jejuni during Co-Culture with the Hyper-Ammonia Producing Anaerobe Clostridium aminophilum SO FOLIA MICROBIOLOGICA LA English DT Article ID RUMEN; BACTERIA; CULTURE AB Campylobacter spp. are a leading bacterial cause of human foodborne illness. When co-cultured in anaerobic Bolton broth with the hyper-ammonia producing bacterium, Clostridium aminophilum, ammonia accumulation was greater and final growth of Campylobacter jejuni was reduced (CFU >= 1.4 log(10)/mL) compared to that obtained by pure culture controls. Co-culture with the less active ammonia-producing saccharolytic Prevotella albensis had no effect on final C. jejuni concentrations. When co-cultured similarly except with the addition of 10 mu mol/L monensin, monensin-susceptible Cl. aminophilum was reduced by 2 to 4 log(10) CFU/mL and concentrations of C. jejuni, which is insensitive to monensin, did not differ from its pure culture control. These results suggest that in the absence of added monensin, the hyper ammonia-producing Cl. aminophilum may be able to outcompete asaccharolytic C. jejuni for amino acid substrates and that this competitive ability was eliminated by addition on monensin. C1 [Anderson, R. C.; Krueger, N. A.; Callaway, T. R.; Edrington, T. S.; Harvey, R. B.; Nisbet, D. J.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX 77845 USA. [Flythe, M. D.] Univ Kentucky, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. RP Anderson, RC (reprint author), ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX 77845 USA. EM Robin.Anderson@ars.usda.gov RI Flythe, Michael/F-2500-2010 OI Flythe, Michael/0000-0002-8868-9169 NR 12 TC 2 Z9 2 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0015-5632 J9 FOLIA MICROBIOL JI Folia Microbiol. PD JUL PY 2010 VL 55 IS 4 BP 309 EP 311 PG 3 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 643EH UT WOS:000281277900001 PM 20680559 ER PT J AU Shih, CK Chen, CM Chen, CYO Liu, JF Lin, HW Chou, HT Li, SC AF Shih, Chun-Kuang Chen, Chiao-Ming Chen, C. -Y. Oliver Liu, Jen-Fang Lin, Hui-Wen Chou, Hung-Tsung Li, Sing-Chung TI Riboflavin protects mice against liposaccharide-induced shock through expression of heat shock protein 25 SO FOOD AND CHEMICAL TOXICOLOGY LA English DT Article DE Vitamin B2; Lipopolysaccharide; Sepsis; Heat shock protein 25; Heat shock factor 1 ID HIGHLY PURIFIED VITAMIN-B-2; LIPOPOLYSACCHARIDE-INDUCED SHOCK; EXPERIMENTAL SEPSIS; IMPROVES SURVIVAL; ENDOTOXIN-SHOCK; LUNG INJURY; CYTOKINE; PLASMA; DEATH; BLOOD AB Riboflavin (vitamin B2) is a water-soluble vitamin essential for normal cellular functions, growth and development. This study aimed to investigate the effects of vitamin B2 on the survival rate, and expressions of tissue heat shock protein 25 (HSP25) and heat shock factor 1 (HSF1) in mice undergoing lipopolysaccharide (LPS) induced shock. Mice were assigned to four groups, saline vehicle, LPS, LPS plus low dose of vitamin B2 (LB2) and LPS plus high dose of vitamin B2 (HB2). Vitamin B2 (1 and 10 mg/kg BW) was administered intraperitoneally at 2 and 0 h before the i.p. administration of LPS. At the end of the experiment, the survival rate monitored was 10, 20, 60, and 100% for LPS, LB2, HB2, and saline mice, respectively. HSP25 expressions in the heart and lung were significantly enhanced in a time-dependent manner in the HB2 mice as compared to the saline mice (p<0.05), but not altered in the LB2 mice. In the HB2 mice, plasma riboflavin concentrations reached 300 nM at 6 h post LPS and returned to the 0 h level at 72 h. The results showed that high dose of riboflavin could decrease LPS-induced mortality through an increased expression of HSP25. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Shih, Chun-Kuang; Liu, Jen-Fang; Li, Sing-Chung] Taipei Med Univ, Sch Nutr & Hlth Sci, Taipei 110, Taiwan. [Chen, Chiao-Ming] Shih Chien Univ, Dept Food Sci Nutr & Nutraceut Biotechnol, Taipei 110, Taiwan. [Chen, C. -Y. Oliver] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lin, Hui-Wen] Taipei Med Univ, Biostat Res & Consulting Ctr, Taipei 110, Taiwan. [Chou, Hung-Tsung] Taipei Med Univ, Div Emergency Med, Wan Fang Hosp, Taipei 110, Taiwan. RP Li, SC (reprint author), 250 Wu Hsing St, Taipei 110, Taiwan. EM sinchung@tmu.edu.tw FU National Science Council [NSC 94-2320-B-038-044]; Taipei Medical University-Wan Fang Hospital, Taiwan, ROC [94TMU-WFH-218] FX This study was supported by a research Grant NSC 94-2320-B-038-044 from the National Science Council and sponsored by the Taipei Medical University-Wan Fang Hospital (94TMU-WFH-218), Taiwan, ROC. We also thank research assistant Ms. Chia-Wen Chou and Yung-Ching Chan for the work. NR 35 TC 7 Z9 8 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0278-6915 J9 FOOD CHEM TOXICOL JI Food Chem. Toxicol. PD JUL PY 2010 VL 48 IS 7 BP 1913 EP 1918 DI 10.1016/j.fct.2010.04.033 PG 6 WC Food Science & Technology; Toxicology SC Food Science & Technology; Toxicology GA 620VY UT WOS:000279530500022 PM 20430062 ER PT J AU Haley, CC Ong, KL Hedberg, K Cieslak, PR Scallan, E Marcus, R Shin, S Cronquist, A Gillespie, J Jones, TF Shiferaw, B Fuller, C Edge, K Zansky, SM Ryan, PA Hoekstra, RM Mintz, E AF Haley, Clinton C. Ong, Kanyin L. Hedberg, Katrina Cieslak, Paul R. Scallan, Elaine Marcus, Ruthanne Shin, Sanghyuk Cronquist, Alicia Gillespie, Jennifer Jones, Timothy F. Shiferaw, Beletshachew Fuller, Candace Edge, Karen Zansky, Shelley M. Ryan, Patricia A. Hoekstra, Robert M. Mintz, Eric TI Risk Factors for Sporadic Shigellosis, FoodNet 2005 SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ACTIVE SURVEILLANCE; UNITED-STATES; OUTBREAK; SONNEI; FLEXNERI; INFECTIONS; TRENDS; TRANSMISSION; ENGLAND; ILLNESS AB Background: An estimated 450,000 cases of shigellosis occur annually in the United States. Outbreaks have been associated with food, water, child daycare centers, and men who have sex with men. However, for sporadic infections, which account for the majority of cases, risk exposures are poorly characterized. Methods: Foodborne Diseases Active Surveillance Network (FoodNet) conducts active, laboratory-based shigellosis surveillance in 10 US sites. We interviewed cases with illness onset during 2005 about exposures during the week before symptom onset using a standardized questionnaire. The proportion of patients who denied nonfood risks was used to estimate the burden attributable to foodborne transmission. Results: Overall, 1494 cases were identified. The approximate incidence was 3.9/100,000, with the highest rates among children aged 1-4 years (16.4) and Hispanics (8.4). Of the 929 cases interviewed, 223 (24%) reported international travel in the week before symptom onset. Of the 626 nontraveling cases with complete risk factor information, 298 (48%) reported exposure to daycare or a household member with diarrhea; 99 (16%) reported drinking untreated water or recreational exposure to water; and 16 (3%) reported sexual contact with a person with diarrhea. Two hundred and fifty-nine (41%) denied all nonfood exposures examined. Conclusions: Sporadic shigellosis is most common among young children and Hispanics. Common exposures include international travel and contact with ill persons or daycare. However, more than one-third of US shigellosis cases annually might be due to food consumed in the United States. C1 [Haley, Clinton C.; Hedberg, Katrina; Cieslak, Paul R.; Shiferaw, Beletshachew] Oregon Publ Hlth Div, Off Dis Prevent & Epidemiol, Portland, OR 97232 USA. [Haley, Clinton C.] Epidem Intelligence Serv, Off Director, Off Workforce & Career Dev, Atlanta, GA USA. [Ong, Kanyin L.; Scallan, Elaine; Hoekstra, Robert M.; Mintz, Eric] Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Natl Ctr Infect Dis, Atlanta, GA USA. [Ong, Kanyin L.] US Food Safety & Inspect Serv, ASPH Publ Hlth, USDA, Washington, DC 20250 USA. [Marcus, Ruthanne] Connecticut Emerging Infect Program, New Haven, CT USA. [Shin, Sanghyuk] Calif Emerging Infect Program, Oakland, CA USA. [Cronquist, Alicia] Colorado Emerging Infect Program, Denver, CO USA. [Gillespie, Jennifer] Georgia Emerging Infect Program, Atlanta, GA USA. [Jones, Timothy F.] Tennessee Dept Hlth, Nashville, TN USA. [Fuller, Candace] Minnesota Dept Hlth, St Paul, MN USA. [Edge, Karen] New Mexico Emerging Infect Program, Albuquerque, NM USA. [Zansky, Shelley M.] New York Emerging Infect Program, Albany, NY USA. [Ryan, Patricia A.] Maryland Dept Hlth & Mental Hyg, Baltimore, MD USA. RP Cieslak, PR (reprint author), Oregon Publ Hlth Div, Off Dis Prevent & Epidemiol, Suite 772,800 NE Oregon St, Portland, OR 97232 USA. EM paul.r.cieslak@state.or.us FU Centers for Disease Control and Prevention, National Center for Infectious Diseases; US Department of Agriculture, Food Safety Inspection Service; US Food and Drug Administration, Center for Food Safety and Applied Nutrition; FoodNet Attributions WG FX We thank the FoodNet Attributions WG for their support and guidance. This study was funded by the Centers for Disease Control and Prevention, National Center for Infectious Diseases; US Department of Agriculture, Food Safety Inspection Service; and US Food and Drug Administration, Center for Food Safety and Applied Nutrition. NR 34 TC 5 Z9 8 U1 2 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2010 VL 7 IS 7 BP 741 EP 747 DI 10.1089/fpd.2009.0448 PG 7 WC Food Science & Technology SC Food Science & Technology GA 619TH UT WOS:000279452700001 PM 20113209 ER PT J AU Dowd, SE Crippen, TL Sun, Y Gontcharova, V Youn, E Muthaiyan, A Wolcott, RD Callaway, TR Ricke, SC AF Dowd, Scot E. Crippen, Tawni L. Sun, Yan Gontcharova, Viktoria Youn, Eun Muthaiyan, Arunachalam Wolcott, Randall D. Callaway, Todd R. Ricke, Steven C. TI Microarray Analysis and Draft Genomes of Two Escherichia coli O157:H7 Lineage II Cattle Isolates FRIK966 and FRIK2000 Investigating Lack of Shiga Toxin Expression SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID HEMORRHAGIC COLITIS; ANTIVIRAL ACTIVITY; SHIGELLA DIARRHEA; O157-H7 STRAINS; PHAGE; GENES; VIRULENCE; SEQUENCE; BOVINE; PATHOGENESIS AB The existence of two separate genetic lineages of Escherichia coli O157:H7 has previously been reported, and research indicates that lineage I could be more pathogenic toward human hosts than lineage II. We have previously shown that lineage I as a group expresses higher levels of Shiga toxin 2 (Stx2) than lineage II. To help evaluate why lineage II strains do not express appreciable levels of this toxin, whole-genome microarrays were performed using Agilent custom microarrays. Gene expression of the two representative bovine lineage II strains (FRIK966 and FRIK2000) were compared with gene expression of E. coli O157:H7 EDL933 (lineage I clinical type strain). Missing or differentially expressed genes and pathways were identified. Quantitative reverse transcription-polymerase chain reaction was performed to validate the microarray data. Draft genomes of FRIK966 and FRIK2000 were sequenced using Roche Applied Science/454 GS-FLX technology shotgun and paired-end approaches followed by de novo assembly. These assemblies were compared with the lineage I genome sequences from E. coli O157:H7 EDL933. The bacteriophage 933W, which encodes the Stx2 genes, showed a notable repression in gene expression. Polymerase chain reaction primers, based upon EDL933 genomic information, were also designed against all of the potentially missing genes of this bacteriophage. Most of the structural genes associated with the bacteriophage were found to be absent from the genome of the two bovine strains. These analyses, combined with evaluation of the genomic information, suggest that transposon (IS629) rearrangements may be associated with disruption of the bacteriophage genome in the FRIK strains. The results support the hypothesis that lineage II strains may be less of a risk as human foodborne pathogens. The microarray and genome data have been made available to the scientific community to allow continuing analysis of these cattle-isolated lineage II genomes and their gene expression. C1 [Dowd, Scot E.; Sun, Yan; Gontcharova, Viktoria; Wolcott, Randall D.] Res & Testing Lab, Lubbock, TX 79407 USA. [Crippen, Tawni L.; Callaway, Todd R.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX USA. [Youn, Eun] Texas Tech Univ, Dept Comp Sci, Lubbock, TX 79409 USA. [Muthaiyan, Arunachalam; Ricke, Steven C.] Univ Arkansas, Ctr Food Safety IFSE, Fayetteville, AR 72701 USA. [Muthaiyan, Arunachalam; Ricke, Steven C.] Univ Arkansas, Dept Food Sci, Fayetteville, AR 72703 USA. [Ricke, Steven C.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. RP Dowd, SE (reprint author), Res & Testing Lab, 4321 Marsha Sharp Fwy, Lubbock, TX 79407 USA. EM sdowd@pathogenresearch.org RI Muthaiyan, Arunachalam/B-6442-2011 FU Research and Testing Laboratory (Lubbock, TX) FX Charles Kasper for the original isolation of these bacteria, Andrew Benson for providing the strains, and the team of Research and Testing for helping with all aspects of the study. This study was conducted using internal research monies from Research and Testing Laboratory (Lubbock, TX) as well as support from authors. NR 53 TC 9 Z9 9 U1 0 U2 1 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2010 VL 7 IS 7 BP 763 EP 773 DI 10.1089/fpd.2009.0482 PG 11 WC Food Science & Technology SC Food Science & Technology GA 619TH UT WOS:000279452700004 PM 20156085 ER PT J AU Wang, B Wesley, IV McKean, JD O'Connor, AM AF Wang, Bing Wesley, Irene V. McKean, James D. O'Connor, Annette M. TI Sub-Iliac Lymph Nodes at Slaughter Lack Ability to Predict Salmonella enterica Prevalence for Swine Farms SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID PRODUCTION SYSTEMS; MARKET SWINE; PORK MEAT; PIGS; TYPHIMURIUM; HERDS; INFECTION; OUTBREAK; RISK; SURVEILLANCE AB The aim of this study was to assess the value of deep systemic sub-iliac lymph nodes collected at slaughter as predictors of Salmonella prevalence in live hogs. An observational study was conducted on 24 farms from September 2006 to February 2009. At least one cohort of market-weight pigs was visited for each farm. Within each cohort, 30 farm fecal samples on farm and 30 sub-iliac lymph nodes from matched pigs at slaughter were collected. Samples were cultured for Salmonella enterica and serotyped by conventional methods. Overall, 3.4% (51 of 1490) of farm feces and 0.06% (1 of 1739) of sub-iliac lymph nodes were Salmonella positive; 71.4% (15 of 21) of farms had at least one positive fecal sample, and 4.2% (1 of 24) had at least one positive sub-iliac lymph node. The median within-farm prevalence of Salmonella in farm fecal samples was 1.7%, ranging from 0% to 38.3%; for sub-iliac lymph nodes the median was 0%, ranging from 0% to 1.1%. The median within-cohort prevalence in farm fecal samples was 0%, ranging from 0% to 43.3%; for sub-iliac lymph nodes the median was 0%, ranging from 0% to 4%. The predominant serotype detected was Derby, followed by Anatum and Typhimurium (Copenhagen). Salmonella Braenderup was recovered from the sub-iliac lymph node. The low detection rate of Salmonella in sub-iliac lymph nodes (0.06%) limits its usefulness as a dependable predictor of Salmonella contamination originating on farm (3.4%). C1 [Wang, Bing; McKean, James D.; O'Connor, Annette M.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA. [Wesley, Irene V.] ARS, Preharvest Food Safety & Enter Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP O'Connor, AM (reprint author), Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA. EM oconnor@iastate.edu RI O'Connor, Annette/E-5591-2010 OI O'Connor, Annette/0000-0003-0604-7822 FU USDA-CSREES [2005-35212-15929] FX We thank all the hog farms and producers who participated. We thank the swine product safety laboratories in Iowa State University for collecting and determining Salmonella enterica. Funding was provided by USDA-CSREES Grant 2005-35212-15929. NR 36 TC 5 Z9 5 U1 0 U2 1 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2010 VL 7 IS 7 BP 795 EP 800 DI 10.1089/fpd.2009.0459 PG 6 WC Food Science & Technology SC Food Science & Technology GA 619TH UT WOS:000279452700007 PM 20187754 ER PT J AU Green, AL Dargatz, DA Wagner, BA Fedorka-Cray, PJ Ladely, SR Kopral, CA AF Green, Alice L. Dargatz, David A. Wagner, Bruce A. Fedorka-Cray, Paula J. Ladely, Scott R. Kopral, Chris A. TI Analysis of Risk Factors Associated with Salmonella spp. Isolated from US Feedlot Cattle SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ESCHERICHIA-COLI O157-H7; ORGANIC DAIRY FARMS; ANTIMICROBIAL RESISTANCE; UNITED-STATES; WET CORN; TYPHIMURIUM; PERFORMANCE; PREVALENCE; ENTERICA; STRESS AB Contamination can occur at a number of stages during farm-to-fork processing. Preharvest intervention is an ongoing area of interest in reduction of risk of foodborne illness. This study examined risk factors associated with detection of Salmonella from cattle in U.S. feedlots. During two visits to 73 feedlots, 25 fresh fecal samples were collected from each of three pen floors. Associations between management and demographic factors and culture status were evaluated using logistic regression. Factors positively associated with culture-positive status included brewers' grains (odds ratio [OR] 26.35; confidence interval [CI], 10.33-67.20), corn gluten (OR = 10.35; CI, 5.98-17.91), or cottonseed hulls (OR = 8.34; CI, 3.58-19.42) in the ration, and sourcing animals in a pen from multiple herds of origin (OR = 5.17; CI, 2.32-11.51). Factors negatively associated with positive culture status included urea (OR = 0.27; CI, 0.16-0.44), alfalfa, clover, or sorghum silage (OR = 0.31; CI, 0.12-0.79), and antimicrobials of the tetracycline class in the ration (within 2 weeks before sampling, OR = 0.04 and CI, 0.02-0.09; more than 2 weeks before sampling, OR = 0.23 and CI, 0.06-0.80). Since 18.3% of positive samples were on a single operation, a second model was constructed after excluding data from this operation. Three additional variables were retained in the second model, including grain-processing method (OR for dry roll, cracked, or unprocessed grain = 2.99; CI, 1.55-5.75), soybean meal (OR = 2.74; CI, 1.58-4.75), and use of a coccidiostat in the ration (OR for no coccidiostat = 4.50; CI, 2.03-10.01). Considering the increasing use of by-products of the biofuel industry as feeds, further investigation of the association between feeding brewers' grains and corn gluten and Salmonella recovery is warranted. C1 [Green, Alice L.] Tennessee Dept Hlth Communicable & Environm Dis S, Nashville, TN 37243 USA. [Dargatz, David A.; Wagner, Bruce A.; Kopral, Chris A.] US Anim & Plant Hlth Inspect Serv, Ctr Epidemiol, Vet Serv, USDA, Ft Collins, CO USA. [Dargatz, David A.; Wagner, Bruce A.; Kopral, Chris A.] US Anim & Plant Hlth Inspect Serv, Ctr Anim Hlth, Vet Serv, USDA, Ft Collins, CO USA. [Fedorka-Cray, Paula J.; Ladely, Scott R.] ARS, Richard B Russell Agr Res Ctr, USDA, Athens, GA USA. RP Green, AL (reprint author), Tennessee Dept Hlth Communicable & Environm Dis S, Cordell Hull Bldg,1st Floor,425 5th Ave N, Nashville, TN 37243 USA. EM alice.l.green@gmail.com NR 45 TC 6 Z9 7 U1 0 U2 8 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2010 VL 7 IS 7 BP 825 EP 833 DI 10.1089/fpd.2007.0068 PG 9 WC Food Science & Technology SC Food Science & Technology GA 619TH UT WOS:000279452700011 PM 20230269 ER PT J AU Callaway, TR Edrington, TS Brabban, A Kutter, E Karriker, L Stahl, C Wagstrom, E Anderson, RC Genovese, K McReynolds, J Harvey, R Nisbet, DJ AF Callaway, Todd R. Edrington, Tom S. Brabban, Andrew Kutter, Elizabeth Karriker, Locke Stahl, Chad Wagstrom, Elizabeth Anderson, Robin C. Genovese, Ken McReynolds, Jack Harvey, Roger Nisbet, David J. TI Occurrence of Salmonella-Specific Bacteriophages in Swine Feces Collected from Commercial Farms SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ESCHERICHIA-COLI DIARRHEA; ANTIBIOTIC-RESISTANCE; PRODUCTION SYSTEMS; BROILER-CHICKENS; FEEDLOT CATTLE; NORTH-CAROLINA; UNITED-STATES; WASTE-WATER; PIGS; PREVALENCE AB Salmonella is one of the leading causes of human foodborne illness and is associated with swine production. Bacteriophages are naturally occurring viruses that prey on bacteria and have been suggested as a potential intervention strategy to reduce Salmonella levels in food animals on the farm and in the lairage period. If phages are to be used to improve food safety, then we must understand the incidence and natural ecology of both phages and their hosts in the intestinal environment. This study investigates the incidence of phages that are active against Salmonella spp. in the feces of commercial finishing swine. Fecal samples (n = 60) were collected from each of 10 commercial swine finishing operations. Samples were collected from 10 randomly selected pens throughout each operation; a total of 600 fecal samples were collected. Salmonella spp. were found in 7.3% (44/600) of the fecal samples. Bacteriophages were isolated from fecal samples through two parallel methods: (1) initial enrichment in Salmonella Typhimurium; (2) initial enrichment in Escherichia coli B (an indicator strain), followed by direct spot testing against Salmonella Typhimurium. Bacteriophages active against Salmonella Typhimurium were isolated from 1% (6/600) of the individual fecal samples when initially enriched in Salmonella Typhimurium, but E. coli B-killing phages were isolated from 48.3% (290/600) of the fecal samples and only two of these phages infected Salmonella Typhimurium on secondary plating. Collectively, our results indicate that bacteriophages are widespread in commercial swine, but those capable of killing Salmonella Typhimurium may be present at relatively low population levels. These results indicate that phages (predator) populations may vary along with Salmonella (prey) populations; and that phages could potentially be used as a food safety pathogen reduction strategy in swine. C1 [Callaway, Todd R.; Edrington, Tom S.; Anderson, Robin C.; Genovese, Ken; McReynolds, Jack; Harvey, Roger; Nisbet, David J.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX 77845 USA. [Brabban, Andrew; Kutter, Elizabeth] Evergreen State Coll, Sci Inquiry Planning Unit, Olympia, WA 98505 USA. [Karriker, Locke] Iowa State Univ, Coll Vet Med, Vet Diagnost & Prod Anim Med Dept, Ames, IA USA. [Stahl, Chad] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Wagstrom, Elizabeth] Natl Pork Board, Des Moines, IA USA. RP Callaway, TR (reprint author), ARS, Food & Feed Safety Res Unit, USDA, 2881 F&B Rd, College Stn, TX 77845 USA. EM todd.callaway@ars.usda.gov FU U.S. pork producers through the National Pork Board FX This project was funded in part by the U.S. pork producers through the National Pork Board. NR 49 TC 12 Z9 12 U1 1 U2 2 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2010 VL 7 IS 7 BP 851 EP 856 DI 10.1089/fpd.2009.0512 PG 6 WC Food Science & Technology SC Food Science & Technology GA 619TH UT WOS:000279452700014 PM 20230290 ER EF