FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Farris, RE Ruiz-Arce, R Ciomperlik, M Vasquez, JD Deleon, R AF Farris, R. E. Ruiz-Arce, R. Ciomperlik, M. Vasquez, J. D. Deleon, R. TI Development of a ribosomal DNA ITS2 marker for the identification of the thrips, Scirtothrips dorsalis SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Chilli thrips; invasive pest; PCR primers; molecular diagnostics ID INTERNAL TRANSCRIBED SPACER-2; FRAGMENT-LENGTH-POLYMORPHISM; THYSANOPTERA; POPULATIONS; CULICIDAE; SEQUENCES; COMPLEX; DIPTERA; REGION; AUSTRALIA AB The thrips Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) is an invasive pest that poses a significant economical threat to U. S. agriculture and trade. In this study, DNA sequence data and polymerase chain reaction (PCR) were utilized to develop a molecular diagnostic marker for S. dorsalis. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA (rDNA) was analyzed from various thrips species, including S. dorsalis. A primer set and polymerase chain reaction cycling parameters were designed for the amplification of a single marker fragment of S. dorsalis ITS2 rDNA. Specificity tests performed on ten thrips species, efficacy tests performed on fifteen S. dorsalis populations, and tests on primer sensitivity and robustness all demonstrated the diagnostic utility of this marker. This diagnostic PCR assay provides a quick, simple, and reliable molecular technique to be used in the identification of S. dorsalis. C1 [Farris, R. E.; Ciomperlik, M.; Vasquez, J. D.; Deleon, R.] CPHST, USDA, Edinburg, TX 78541 USA. [Ruiz-Arce, R.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. RP Farris, RE (reprint author), CPHST, USDA, Mission Lab 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM Roxanne.e.farris@aphis.usda.gov NR 36 TC 9 Z9 11 U1 0 U2 3 PU UNIV ARIZONA PI TUCSON PA LIBRARY C327, TUCSON, AZ 85721 USA SN 1536-2442 J9 J INSECT SCI JI J Insect Sci. PD MAR 28 PY 2010 VL 10 AR 26 PG 15 WC Entomology SC Entomology GA 579GO UT WOS:000276357200001 PM 20578948 ER PT J AU Kumar, L Breakspear, A Kistler, C Ma, LJ Xie, XH AF Kumar, Lokesh Breakspear, Andrew Kistler, Corby Ma, Li-Jun Xie, Xiaohui TI Systematic discovery of regulatory motifs in Fusarium graminearum by comparing four Fusarium genomes SO BMC GENOMICS LA English DT Article ID SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-NIDULANS; YEAST; GENE; DNA; ELEMENTS; REVEALS; BINDING; STRESS; SITES AB Background: Fusarium graminearum (Fg), a major fungal pathogen of cultivated cereals, is responsible for billions of dollars in agriculture losses. There is a growing interest in understanding the transcriptional regulation of this organism, especially the regulation of genes underlying its pathogenicity. The generation of whole genome sequence assemblies for Fg and three closely related Fusarium species provides a unique opportunity for such a study. Results: Applying comparative genomics approaches, we developed a computational pipeline to systematically discover evolutionarily conserved regulatory motifs in the promoter, downstream and the intronic regions of Fg genes, based on the multiple alignments of sequenced Fusarium genomes. Using this method, we discovered 73 candidate regulatory motifs in the promoter regions. Nearly 30% of these motifs are highly enriched in promoter regions of Fg genes that are associated with a specific functional category. Through comparison to Saccharomyces cerevisiae (Sc) and Schizosaccharomyces pombe (Sp), we observed conservation of transcription factors (TFs), their binding sites and the target genes regulated by these TFs related to pathways known to respond to stress conditions or phosphate metabolism. In addition, this study revealed 69 and 39 conserved motifs in the downstream regions and the intronic regions, respectively, of Fg genes. The top intronic motif is the splice donor site. For the downstream regions, we noticed an intriguing absence of the mammalian and Sc poly-adenylation signals among the list of conserved motifs. Conclusion: This study provides the first comprehensive list of candidate regulatory motifs in Fg, and underscores the power of comparative genomics in revealing functional elements among related genomes. The conservation of regulatory pathways among the Fusarium genomes and the two yeast species reveals their functional significance, and provides new insights in their evolutionary importance among Ascomycete fungi. C1 [Breakspear, Andrew; Kistler, Corby] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Kumar, Lokesh; Ma, Li-Jun] Broad Inst, Cambridge, MA USA. [Kumar, Lokesh; Xie, Xiaohui] Univ Calif Irvine, Inst Genom & Bioinformat, Dept Comp Sci, Irvine, CA USA. RP Kistler, C (reprint author), Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM hckist@umn.edu; lijun@broadinstitute.org; xhx@ics.uci.edu OI Ma, Li-Jun/0000-0002-2733-3708 FU USDA [2005-35600-16405]; NSF EnGen [0723451]; NSF [DBI0846218] FX We thank the Broad Institute for making the genomes of the three Fusarium species available before publication. LK and LJM are supported by USDA 2005-35600-16405; CK and AB are supported by NSF EnGen 0723451; and XX is supported by NSF DBI0846218. NR 42 TC 11 Z9 12 U1 1 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 26 PY 2010 VL 11 AR 208 DI 10.1186/1471-2164-11-208 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 581ZS UT WOS:000276565200001 PM 20346147 ER PT J AU Erupe, ME Liberman-Martin, A Silva, PJ Malloy, QGJ Yonis, N Cocker, DR Purvis-Roberts, KL AF Erupe, Mark E. Liberman-Martin, Allegra Silva, Philip J. Malloy, Quentin G. J. Yonis, Naomi Cocker, David R., III Purvis-Roberts, Kathleen L. TI Determination of methylamines and trimethylamine-N-oxide in particulate matter by non-suppressed ion chromatography SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article DE Non-suppressed ion chromatography; Trimethylamine-N-oxide; Aerosols; Alkylamines; Organic nitrogen ID PULSED AMPEROMETRIC DETECTION; SECONDARY AEROSOL FORMATION; VOLATILE ORGANIC-COMPOUNDS; BIOGENIC-AMINES; CONDUCTIVITY DETECTION; ALIPHATIC-AMINES; DAIRY; SEPARATION; ELECTRODE; AMMONIA AB An ion chromatography method with non-suppressed conductivity detection was developed for the simultaneous determination of methylamines (methylamine, dimethylamine, trimethylamine) and trimethylamine-N-oxide in particulate matter air samples. The analytes were well separated by means of cation-exchange chromatography using a 3 mM nitric acid/3.5% acetonitrile (v/v) eluent solution and a Metrosep C 2 250 (250 mm x 4 mm i.d.) separation column. The effects of the different chromatographic parameters on the separation were also investigated. Detection limits of methylamine, dimethylamine, trimethylamine,and trimethylamine-N-oxide were 43, 46, 76 and 72 mu g/L, respectively. The relative standard deviations of the retention times were between 0.42% and 1.14% while the recoveries were between 78.8% and 88.3%. The method is suitable for determining if methylamines and trimethylamine-N-oxide are a significant component of organic nitrogen aerosol in areas with high concentration of these species. (C) 2010 Elsevier B.V. All rights reserved. C1 [Liberman-Martin, Allegra; Yonis, Naomi; Purvis-Roberts, Kathleen L.] Claremont Mckenna Coll, Joint Sci Dept, WM Keck Sci Ctr, Claremont, CA 91711 USA. [Liberman-Martin, Allegra; Yonis, Naomi; Purvis-Roberts, Kathleen L.] Pitzer Coll, Joint Sci Dept, WM Keck Sci Ctr, Claremont, CA 91711 USA. [Liberman-Martin, Allegra; Yonis, Naomi; Purvis-Roberts, Kathleen L.] Scripps Coll, Joint Sci Dept, WM Keck Sci Ctr, Claremont, CA 91711 USA. [Erupe, Mark E.; Silva, Philip J.] Utah State Univ, Dept Chem & Biochem, Logan, UT 84322 USA. [Silva, Philip J.] ARS, USDA, Bowling Green, KY 42104 USA. [Malloy, Quentin G. J.; Cocker, David R., III] Univ Calif Riverside, Dept Environm Chem & Engn, Riverside, CA 92521 USA. [Malloy, Quentin G. J.; Cocker, David R., III] Univ Calif Riverside, Coll Engn, Ctr Environm Res & Technol, Riverside, CA 92521 USA. RP Purvis-Roberts, KL (reprint author), Claremont Mckenna Coll, Joint Sci Dept, WM Keck Sci Ctr, 925 N Mills Ave, Claremont, CA 91711 USA. EM kpurvis@jsd.claremont.edu RI Cocker, David/F-4442-2010; OI Cocker, David/0000-0002-0586-0769; Liberman-Martin, Allegra/0000-0002-8447-905X FU National Science Foundation [ATM-0735396, ATM-0449778, DUE-0525574]; Kenneth T. and Eileen L. Norris Foundation FX This project was supported by the National Science Foundation undergrants ATM-0735396, ATM-0449778, and DUE-0525574, and the Kenneth T. and Eileen L. Norris Foundation. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 28 TC 16 Z9 16 U1 6 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR A JI J. Chromatogr. A PD MAR 26 PY 2010 VL 1217 IS 13 BP 2070 EP 2073 DI 10.1016/j.chroma.2010.01.066 PG 4 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 573OI UT WOS:000275923000011 PM 20181351 ER PT J AU Jiang, HX Campbell, M Blanco, M Jane, JL AF Jiang, Hongxin Campbell, Mark Blanco, Mike Jane, Jay-Lin TI Characterization of maize amylose-extender (ae) mutant starches: Part II. Structures and properties of starch residues remaining after enzymatic hydrolysis at boiling-water temperature SO CARBOHYDRATE POLYMERS LA English DT Article DE Resistant starch; Starch structure; Amylose-extender mutant; High-amylose maize starch; Long-chain double-helical crystallite ID INTERNAL STRUCTURE; RESISTANT STARCH; AMYLOPECTIN; GRANULES; GELATINIZATION; LOCATION; SITES; SIZE AB GEMS-0067 maize ae-line starches developed by the Germplasm Enhancement of Maize (GEM) project consist of 39.4-43.2% resistant starch (RS). which is larger than the existing ne-line starches of H99ae, OH43ae, B89ae, and B84ae ( 11.5-19.1%) as reported in part I of the study. The objective Of this Study was to understand the mechanism of the RS formation in the GEMS-0067 ae-line starch. In the Current study, we analyzed the structures and properties of the RS residues that remained after enzymatic hydrolysis of the ae-line starches at 95-100 degrees C. The RS residues consisted of two major components: large starch molecules of average degrees of polymerization (DP) 840-951 with a few branches and small starch molecules (average DP 59-74) with mostly linear chains. All the RS residues had a semi-crystalline structure with the B-type polymorph and displayed high onset (100.7-107.7 degrees C), peak (118.6-121.4 degrees C), and conclusion (139.7-158.8 degrees C) gelatinization temperatures. After the maize ne-mutant starches were defatted with methanol, the RS contents decreased to 27.8-28.9% for the GEMS-0067 ae-line starches and 9.0-11.0% for the existing ae-line starches. The RS residues were attributed to the presence of long-chain double-helical crystallites derived from amylose and the intermediate component (IC). These crystallites present in native ae-line starches had gelatinization temperatures above 100 degrees C and maintained the semi-crystalline structures after enzymatic hydrolysis at 95-100 degrees C. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Jiang, Hongxin; Jane, Jay-Lin] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. [Campbell, Mark] Truman State Univ, Kirksville, MO 63501 USA. [Blanco, Mike] USDA ARS, Plant Intro Res Unit, Ames, IA 50011 USA. RP Jane, JL (reprint author), Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. EM jjane@iastate.edu FU USDA-ARS FX The authors thank the USDA-ARS GEM project for the support oil this research, Microscopy and NanoImaging Facility at Iowa State University for the microscopic study, and Dr. Schlorholtz for help on X-ray analysis. NR 30 TC 54 Z9 56 U1 2 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD MAR 25 PY 2010 VL 80 IS 1 BP 1 EP 12 DI 10.1016/j.carbpol.2009.10.060 PG 12 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 562UN UT WOS:000275080600001 ER PT J AU Lindsey, R Ha, Y Momany, M AF Lindsey, Rebecca Ha, Youngsil Momany, Michelle TI A Septin from the Filamentous Fungus A. nidulans Induces Atypical Pseudohyphae in the Budding Yeast S. cerevisiae SO PLOS ONE LA English DT Article ID SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-NIDULANS; GENE FAMILY; BUD NECK; GROWTH; ROLES; LOCALIZATION; ORGANIZATION; CYTOKINESIS; EMERGENCE AB Background: Septins, novel cytoskeletal proteins, form rings at the bases of emerging round buds in yeasts and at the bases of emerging elongated hyphal initials in filamentous fungi. Methodology/Principal Findings: When introduced into the yeast Saccharomyces cerevisiae, the septin AspC from the filamentous fungus Aspergillus nidulans induced highly elongated atypical pseudohyphae and spore-producing structures similar to those of hyphal fungi. AspC induced atypical pseudohyphae when S. cerevisiae pseudohyphal or haploid invasive genes were deleted, but not when the CDC10 septin gene was deleted. AspC also induced atypical pseudohyphae when S. cerevisiae genes encoding Cdc12-interacting proteins Bem4, Cla4, Gic1 and Gic2 were deleted, but not when BNI1, a Cdc12-interacting formin gene, was deleted. AspC localized to bud and pseudohypha necks, while its S. cerevisiae ortholog, Cdc12, localized only to bud necks. Conclusions/Significance: Our results suggest that AspC competes with Cdc12 for incorporation into the yeast septin scaffold and once there alters cell shape by altering interactions with the formin Bni1. That introduction of the A. nidulans septin AspC into S. cerevisiae induces a shift from formation of buds to formation of atypical pseudohyphae suggests that septins play an important role in the morphological plasticity of fungi. C1 [Lindsey, Rebecca; Ha, Youngsil; Momany, Michelle] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA. RP Lindsey, R (reprint author), USDA, ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Agr Res Ctr, Athens, GA USA. EM momany@plantbio.uga.edu RI Momany, Michelle/L-2327-2016; OI Momany, Michelle/0000-0001-7110-063X FU NSF [MCB0211787] FX This work was supported by NSF grant MCB0211787 to MM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 33 TC 10 Z9 11 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 25 PY 2010 VL 5 IS 3 AR e9858 DI 10.1371/journal.pone.0009858 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 574DS UT WOS:000275969500005 PM 20360845 ER PT J AU Burke, JM Soli, F Miller, JE Terrill, TH Wildeus, S Shaik, SA Getz, WR Vanguru, M AF Burke, J. M. Soli, F. Miller, J. E. Terrill, T. H. Wildeus, S. Shaik, S. A. Getz, W. R. Vanguru, M. TI Administration of copper oxide wire particles in a capsule or feed for gastrointestinal nematode control in goats SO VETERINARY PARASITOLOGY LA English DT Article DE Copper oxide; Gastrointestinal nematodes; Goat ID HAEMONCHUS-CONTORTUS; ANTHELMINTIC RESISTANCE; LAMBS; SHEEP; INFECTION; FARMS AB Widespread anthelmintic resistance in small ruminants has necessitated alternative means of gastrointestinal nematode (GIN) control. The objective was to determine the effectiveness of copper oxide wire particles (COWP) administered as a gelatin capsule or in a feed supplement to control GIN in goats. in four separate experiments, peri-parturient does (n = 36), yearling does (n = 25), weaned kids (n = 72), and yearling bucks (n = 16) were randomly assigned to remain untreated or administered 2 g COWP in a capsule (in Experiments 1, 2, and 3) or feed supplement (all experiments). Feces and blood were collected every 7 days between Days 0 and 21 (older goats) or Day 42 (kids) for fecal egg counts (FEC) and blood packed cell volume (PCV) analyses. A peri-parturient rise in FEC was evident in the untreated does, but not the COWP-treated does (COWP x date, P < 0.02). In yearling does, FEC of the COWP-treated does tended to be lower than the untreated (COWP, P < 0.02). FEC of COWP-treated kids were reduced compared with untreated kids (COWP x date, P < 0.001). FEC of treated and untreated bucks were similar, but Haemonchus contortus was not the predominant nematode in these goats. However, total worms were reduced in COWP-fed bucks (P < 0.03). In summary, it appeared that COWP in the feed was as effective as COWP in a gelatin capsule to reduce FEC in goats. COWP administration may have a limited effect where H. contortus is not the predominant nematode. Published by Elsevier B.V. C1 [Burke, J. M.] ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Soli, F.; Terrill, T. H.; Shaik, S. A.; Getz, W. R.; Vanguru, M.] Ft Valley State Univ, Ft Valley, GA 31030 USA. [Miller, J. E.] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA. [Miller, J. E.] Louisiana State Univ, Dept Anim Sci, Baton Rouge, LA 70803 USA. [Miller, J. E.] Louisiana State Univ, Dept Vet Sci, Baton Rouge, LA 70803 USA. [Wildeus, S.] Virginia State Univ, Petersburg, VA 23806 USA. RP Burke, JM (reprint author), ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. EM joan.burke@ars.usda.gov FU USDA [2005-38814-16429]; CSREES [2005-51300-02392]; Southern Region SARE Program [LS08-204] FX This research was supported by the USDA 1890 Institution Teaching and Research Capacity Building Grants Program (Award No. 2005-38814-16429), USDA, CSREES, Integrated Organic Program (Project No. 2005-51300-02392) and the Southern Region SARE Program (Project No. LS08-204). The authors greatly appreciate the efforts of G. Robson, J. Cherry, and L. Rowland. NR 14 TC 8 Z9 8 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD MAR 25 PY 2010 VL 168 IS 3-4 BP 346 EP 350 DI 10.1016/j.vetpar.2009.10.027 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 581KD UT WOS:000276520400027 PM 19945224 ER PT J AU Nafria, JMN Favret, C Durante, MPM Sano, M Akimoto, SI Barbagallo, S Chakrabarti, S Miller, GL Hidalgo, NP Qiao, GX Stekolshchikov, AV Wegierek, P AF Nieto Nafria, Juan M. Favret, Colin Mier Durante, M. Pilar Sano, Masakazu Akimoto, Shin-Ichi Barbagallo, Sebastiano Chakrabarti, Samiran Miller, Gary L. Perez Hidalgo, Nicolas Qiao, Ge-Xia Stekolshchikov, Andrey V. Wegierek, Piotr TI Type species of genera in Aphididae (Hemiptera Sternorrhyncha) with two new generic synonymies SO ZOOTAXA LA English DT Letter C1 [Nieto Nafria, Juan M.; Mier Durante, M. Pilar; Perez Hidalgo, Nicolas] Univ Leon, Dept Biodiversidad & Geston Ambiental, E-24071 Leon, Spain. [Favret, Colin] AphidNet LLC, Gaithersburg, MD 20879 USA. [Sano, Masakazu; Akimoto, Shin-Ichi] Hokkaido Univ, Lab Systemat Entomol, Dept Systemat & Ecol, Grad Sch Agr,Kita Ku, Sapporo, Hokkaido 0608589, Japan. [Barbagallo, Sebastiano] Univ Catania, Dipartimento Sci & Tecnol Fitosanit, I-95123 Catania, Italy. [Chakrabarti, Samiran] Univ Kalyani, Biosystemat Res Unit, Dept Zool, Kalyani 741235, W Bengal, India. [Miller, Gary L.] Agr Res Serv, USDA, Systemat Entomol Lab, Beltsville, MD 20705 USA. [Qiao, Ge-Xia] Chinese Acad Sci, Key Lab Zool Systemat & Evolut, Inst Zool, Beijing 100101, Peoples R China. [Stekolshchikov, Andrey V.] Russian Acad Sci, Lab Insect Taxon, Inst Zool, St Petersburg 199034, Russia. [Wegierek, Piotr] Univ Slaski, Katedra Zool, PL-40007 Katowice, Poland. RP Nafria, JMN (reprint author), Univ Leon, Dept Biodiversidad & Geston Ambiental, E-24071 Leon, Spain. EM jmnien@unileon.es RI Favret, Colin/F-7726-2012; Stekolshchikov, Andrey/G-4725-2015 OI Favret, Colin/0000-0001-6243-3184; NR 15 TC 0 Z9 0 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 25 PY 2010 IS 2410 BP 65 EP 68 PG 4 WC Zoology SC Zoology GA 574DP UT WOS:000275969200006 ER PT J AU Joseph, JA Bielinski, DF Fisher, DR AF Joseph, James A. Bielinski, Donna F. Fisher, Derek R. TI Blueberry Treatment Antagonizes C-2 Ceramide-Induced Stress Signaling in Muscarinic Receptor-Transfected COS-7 Cells SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Ceramide; aging; oxidative stress; blueberry polyphenols; muscarinic receptors ID AMYLOID PRECURSOR PROTEIN; OXIDATIVE STRESS; ALZHEIMERS-DISEASE; PC12 CELLS; CALCIUM DYSREGULATION; CELLULAR SENESCENCE; GENE-EXPRESSION; BETA-PEPTIDE; APOPTOSIS; NEURONS AB Previous research has shown that muscarinic receptors (MAChRs) show loss of sensitivity in aging and AD and are selectively sensitive to oxidative stress (OS). Thus, COS-7 cells transfected (tn) with MACHR subtype M1 show > OS sensitivity [as reflected in the ability of the cell to extrude or sequester Ca(2+) following depolarization (recovery) by oxotremorine (oxo) and exposure to dopamine (DA) or amyloid beta (A beta)] than M3-transfected COS-7 cells. Blueberry (BB) extract pretreatment prevented these deficits. Research has also indicated that C2 ceramide (Cer) has several age-related negative cellular effects (e.g., OS). When these cells were treated with Cer, the significant decrements in the ability of both types of in cells to initially respond to oxo were antagonized by BB treatment. Present experiments assessed signaling mechanisms involved in BB protection in the presence or absence of DA, A beta, and/or Cer in this model. Thus, control or BB-treated M1 and M3 tn COS-7 cells were exposed to DA or A beta(42) in the presence or absence of Cer. Primarily, results showed that the effects of DA or A beta(42) were to increase stress (e.g., PKC gamma, p38MAPK) and protective signals (e.g., pMAPK). Cer also appeared to raise several of the stress and protective signals in the absence of the other stressors, including PKC gamma, pJNK, pNf kappa B, p53, and p38MAPK, while not significantly altering MAPK, or Akt. pArc was, however, increased by Cer in both types of transfected cells. The protective effects of BB when combined with Cer generally showed greater protection when BB extract was applied prior to Cer, except for one protective signal (pArc) where a greater effect was seen in the M3 cells exposed to A beta(42). In the absence of the A beta 42 or DA, for several of the stress signals (e.g., pNf kappa B, p53), BB lowered their Cer-induced increases in M1- and M3-transfected cells. We are exploring these interactions further, but it is clear that increases in ceramide, to the same levels as are seen in aging, can have profound effects on calcium clearance and signaling during oxidative stress. C1 [Joseph, James A.; Bielinski, Donna F.; Fisher, Derek R.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Joseph, JA (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM james.joseph@ars.usda.gov NR 61 TC 6 Z9 6 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3380 EP 3392 DI 10.1021/jf9039155 PG 13 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700021 PM 20178393 ER PT J AU Han, JC Liu, KS AF Han, Jianchun Liu, Keshun TI Changes in Composition and Amino Acid Profile during Dry Grind Ethanol Processing from Corn and Estimation of Yeast Contribution toward DDGS Proteins SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE DDGS; distiller grains; amino acid; yeast; proteins; composition; changes; dry grind process; ethanol; corn ID DISTILLERS DRIED GRAINS; PARTICLE-SIZE DISTRIBUTION; COLOR PROPERTIES; SOLUBLES DDGS; PRODUCTS; QUALITY AB Three sets of samples, consisting of ground corn, yeast, intermediate products, and DDGS, were provided by three commercial dry grind ethanol plants in Iowa and freeze dried before chemical analysis. On average, ground corn contained 70.23% starch, 7.65% protein, 3.26% oil, 1.29% ash, 87.79% total carbohydrate (CHO), and 17.57% total nonstarch CHO, dry matter basis. Results from Plant 1 samples showed that compared to ground corn, there was a slight but significant increase in the contents of protein, amino acids (AA), oil, and ash before fermentation, although starch/dextrin decreased sharply upon saccharification. After fermentation, starch content further decreased to about 6.0%, while protein, oil, and ash contents increased over 3-fold. AA increased 2.0-3.5-fold. Total CHO content decreased by 40%, and the content of total nonstarch CHO increased over 2.5-fold. Concentrations of these attributes fluctuated slightly in the remaining downstream products, but oil and ash were concentrated in thin stillage, while protein was concentrated in distiller grains upon centrifugation. When AA composition is expressed in relative % (protein basis), its changes did not follow that of protein concentration, but the influence of yeast AA profiles on those of downstream products became apparent. Accordingly, a multiple linear regression model for the AA profile of a downstream product as a function of AA profiles of ground corn and yeast was proposed. Regression results indicated that, with an r(2) = 0.95, yeast contributed about 20% toward DDGS proteins, and the rest came from corn. Data from Plants 2 and 3 confirmed those found with Plant 1 samples. C1 [Liu, Keshun] ARS, Grain Chem & Utilizat Lab, Natl Small Grains & Potato Germplasm Res Unit, USDA, Aberdeen, ID 83210 USA. [Han, Jianchun] NE Agr Univ, Coll Food Sci, Harbin 150030, Heilongjian, Peoples R China. RP Liu, KS (reprint author), ARS, Grain Chem & Utilizat Lab, Natl Small Grains & Potato Germplasm Res Unit, USDA, 1691 S 2700 W Aberdeen, Aberdeen, ID 83210 USA. EM Keshun.Liu@ars.usda.gov NR 15 TC 46 Z9 46 U1 3 U2 28 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3430 EP 3437 DI 10.1021/jf9034833 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700027 PM 20166661 ER PT J AU Sattler, SE Funnell-Harris, DL Pedersen, JF AF Sattler, Scott E. Funnell-Harris, Deanna L. Pedersen, Jeffrey F. TI Efficacy of Singular and Stacked brown midrib 6 and 12 in the Modification of Lignocellulose and Grain Chemistry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Sorghum; brown midrib; digestibility; lignocellulosic tissues ID CINNAMYL-ALCOHOL-DEHYDROGENASE; ACID O-METHYLTRANSFERASE; SORGHUM GENETIC STOCKS; BMR-12 GENES; LIGNIN MODIFICATION; FORAGE SORGHUM; LIQUID FUELS; QUALITY; BIOMASS; YIELD AB In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland x RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in vitro dry matter digestibility (IVDMD) compared to those of the wild type (WT). The lignin content of the stacked lignocellulosic tissue was further reduced compared to that of bmr6 or bmr12. Surprisingly, bmr12 modestly increased carbohydrates in lignocellulosic tissue, and bmr6 increased fiber and lignin content in grain. These data indicate that bmr6 and bmr12 have broader effects on plant composition than merely lignin content, which has promising implications for both livestock utilization and bioenergy conversion. C1 [Sattler, Scott E.; Funnell-Harris, Deanna L.; Pedersen, Jeffrey F.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA ARS, Lincoln, NE 68583 USA. [Sattler, Scott E.; Pedersen, Jeffrey F.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Pedersen, JF (reprint author), Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA ARS, Lincoln, NE 68583 USA. EM Jeff.Pedersen@ars.usda.gov NR 42 TC 11 Z9 12 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3611 EP 3616 DI 10.1021/jf903784j PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700052 PM 20175527 ER PT J AU Reddy, KN Bellaloui, N Zablotowicz, RM AF Reddy, Krishna N. Bellaloui, Nacer Zablotowicz, Robert M. TI Glyphosate Effect on Shikimate, Nitrate Reductase Activity, Yield, and Seed Composition in Corn SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Corn; drift Injury; glyphosate; nitrate reductase; seed nitrogen; shikimate; transgenic corn ID SOYBEAN GLYCINE-MAX; ACID ACCUMULATION; SIMULATED DRIFT; IN-CROP; RESISTANT; GLUFOSINATE; HERBICIDES; TOLERANCE; RESPONSES; PLANTS AB When glyphosate is applied to glyphosate-resistant (GR) crops, drift to nonglyphosate-resistant (non-GR) crops may cause significant injury and reduce yields. Tools are needed to quantify injury and predict crop losses. In this study, glyphosate drift was simulated by direct application at 12.5% of the recommended label rate to non-GR corn (Zea mays L.) at 3 or 6 weeks after planting (WAP) during two field seasons in the Mississippi delta region of the southeastern USA. Visual plant injury, shikimate accumulation, nitrate reductase activity, leaf nitrogen, yield, and seed composition were evaluated. Effects were also evaluated in GR corn and GR corn with stacked glufosinate-resistant gene at the recommended label rate at 3 and 6 WAP. Glyphosate at 105 g ae/ha was applied once at 3 or 6 weeks after planting to non-GR corn. Glyphosate at 840 (lower label limit) or 1260 (upper label limit) g ae/ha was applied twice at 3 and 6 WAP to transgenic corn. Glyphosate caused injury (45-55%) and increased shikimate levels (24-86%) in non-GR compared to nontreated corn. In non-GR corn, glyphosate drift did not affect starch content but increased seed protein 8-21% while reducing leaf nitrogen reductase activity 46-64%, leaf nitrogen 7-16%, grain yield 49-54%, and seed oil 18-23%. In GR and GR stacked with glufosinate-resistant corn, glyphosate applied at label rates did not affect corn yield, leaf and seed nitrogen, or seed composition (protein, oil, and starch content). Yet, nitrate reductase activity was reduced 5-19% with glyphosate at 840 + 840 g/ha rate and 8-42% with glyphosate at 1260 + 1260 g/ha rate in both GR and GR stacked corn. These results demonstrate the potential for severe yield loss in non-GR corn exposed to glyphosate drift. C1 [Reddy, Krishna N.; Zablotowicz, Robert M.] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. [Bellaloui, Nacer] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. RP Reddy, KN (reprint author), ARS, Crop Prod Syst Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM krishna.reddy@ars.usda.gov OI Zablotowicz, Robert/0000-0001-8070-1998 NR 33 TC 12 Z9 14 U1 2 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3646 EP 3650 DI 10.1021/jf904121y PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700058 PM 20180575 ER PT J AU Du, XF Finn, CE Qian, MC AF Du, Xiaofen Finn, Chad E. Qian, Michael C. TI Bound Volatile Precursors in Genotypes in the Pedigree of 'Marion' Blackberry (Rubus Sp.) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Blackberry; 'Marion' pedigree; stir bar sorptive extraction (SBSE); solid-phase extraction (SPE); microvial Insert thermal desorption ID FRAGARIA X ANANASSA; AROMA COMPOUNDS; 2,5-DIMETHYL-4-HYDROXY-3(2H)-FURANONE GLUCOSIDE; STRAWBERRY FRUITS; DILUTION ANALYSIS; RASPBERRY FRUIT; IDENTIFICATION; L.; NORISOPRENOIDS; SPECTROMETRY AB Glycosidically bound volatiles and precursors in genotypes representing the pedigree for 'Marion' blackberry were investigated over two growing seasons. The volatile precursors were isolated using a C18 solid-phase extraction column. After enzymatic hydrolysis, the released volatiles were analyzed using stir bar sorptive extraction gas chromatography-mass spectrometry (GC-MS) and direct microvial insert thermal desorption GC-MS. The most abundant volatile precursors in the genotypes were alcohols, followed by shikimic acid derivatives. High amounts of furanone glycosides were also detected, while norisoprenoids only existed in a small amount in blackberries. The volatile precursor composition in the genotypes in the 'Marion' pedigree was very similar to their free volatile distribution. 'Logan' and 'Olallie' predominantly had bound norisoprenoids. Wild 'Himalaya' predominated with terpene alcohol and furaneol glycosides, whereas 'Santiam' and 'Chehalem' contained a high level of terpene alcohol glycosides. A similar inheritance pattern was also observed for some volatile precursors in the genotypes in the 'Marion' pedigree. A high content of linalool, hydroxylinalool, and alpha-ionol glycosides in 'Olallie' and a low content in 'Chehalem' resulted in a moderate level in their offspring 'Marion', while a low content of (E)-linalool oxide precursor in 'Olallie' and a high content in 'Chehalem' also resulted in a moderate level in 'Marion'. However, the concentration of furaneol glycosides in 'Marion' exceeded that of its two parents. C1 [Du, Xiaofen; Qian, Michael C.] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA. [Finn, Chad E.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR 97330 USA. RP Qian, MC (reprint author), Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA. EM michael.qian@oregonstate.edu NR 35 TC 10 Z9 12 U1 2 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3694 EP 3699 DI 10.1021/jf9034089 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700065 PM 20192206 ER PT J AU Bilbao-Sainz, C Wood, RJABDF Williams, TG McHugh, TH AF Bilbao-Sainz, Cristina Wood, Roberto J. Avena-Bustillos Delilah F. Williams, Tina G. McHugh, Tara H. TI Composite Edible Films Based on Hydroxypropyl Methylcellulose Reinforced with Microcrystalline Cellulose Nanoparticles SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Hydroxypropyl methyl cellulose; microcrystalline cellulose; edible films; nanoparticles ID WATER-VAPOR PERMEABILITY; BARRIER PROPERTIES; FATTY-ACID; MECHANICAL-PROPERTIES; STARCH; FIBERS; SURFACTANTS; NANOCOMPOSITES; MICROFIBRILS; COATINGS AB It has been stated that hydroxypropyl methyl cellulose (HPMC) based films have promising applications in the food industry because of their environmental appeal, low cost, flexibility and transparency. Nevertheless, their mechanical and moisture barrier properties should be improved. The aim of this work was to enhance these properties by reinforcing the films with microcrystalline cellulose (MCC) at the nano scale level. Three sizes of MCC nanoparticles were incorporated into HPMC edible films at different concentrations. Identical MCC nanoparticles were lipid coated (LC) prior to casting into HPMC/LC-MCC composite films. The films were examined for mechanical and moisture barrier properties verifying how the addition of cellulose nanoparticles affected the water affinities (water adsorption/desorption isotherms) and the diffusion coefficients. The expected reinforcing effect of the MCC was observed: HPMC/MCC and HPMC/LC-MCC films showed up to 53% and 48% increase, respectively, in tensile strength values in comparison with unfilled HPMC films. Furthermore, addition of unmodified MCC nanoparticles reduced the moisture permeability up to 40% and use of LC-MCC reduced this value up to 50%. Water vapor permeability was mainly influenced by the differences in water solubility of different composite films since, in spite of the increase in water diffusivity values with the incorporation of MCC to HPMC films, better moisture barrier properties were achieved for HPMC/MCC and HPMC/LC-MCC composite films than for HPMC films. C1 [Bilbao-Sainz, Cristina; Wood, Roberto J. Avena-Bustillos Delilah F.; Williams, Tina G.; McHugh, Tara H.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Bilbao-Sainz, C (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. NR 40 TC 36 Z9 37 U1 3 U2 43 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 24 PY 2010 VL 58 IS 6 BP 3753 EP 3760 DI 10.1021/jf9033128 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 570XB UT WOS:000275710700073 PM 20187652 ER PT J AU Seifert, EL Fiehn, O Bezaire, V Bickel, DR Wohlgemuth, G Adams, SH Harper, ME AF Seifert, Erin L. Fiehn, Oliver Bezaire, Veronic Bickel, David R. Wohlgemuth, Gert Adams, Sean H. Harper, Mary-Ellen TI Long-Chain Fatty Acid Combustion Rate Is Associated with Unique Metabolite Profiles in Skeletal Muscle Mitochondria SO PLOS ONE LA English DT Article ID INDUCED INSULIN-RESISTANCE; IN-VIVO; 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE; TRIGLYCERIDE SYNTHESIS; OXIDATIVE-METABOLISM; ADULT CARDIOMYOCYTES; UNCOUPLING PROTEIN-3; CHRONIC EXPOSURE; MALONYL-COA; CYCLE AB Background/Aim: Incomplete or limited long-chain fatty acid (LCFA) combustion in skeletal muscle has been associated with insulin resistance. Signals that are responsive to shifts in LCFA beta-oxidation rate or degree of intramitochondrial catabolism are hypothesized to regulate second messenger systems downstream of the insulin receptor. Recent evidence supports a causal link between mitochondrial LCFA combustion in skeletal muscle and insulin resistance. We have used unbiased metabolite profiling of mouse muscle mitochondria with the aim of identifying candidate metabolites within or effluxed from mitochondria and that are shifted with LCFA combustion rate. Methodology/Principal Findings: Large-scale unbiased metabolomics analysis was performed using GC/TOF-MS on buffer and mitochondrial matrix fractions obtained prior to and after 20 min of palmitate catabolism (n = 7 mice/condition). Three palmitate concentrations (2, 9 and 19 mu M; corresponding to low, intermediate and high oxidation rates) and 9 mu M palmitate plus tricarboxylic acid (TCA) cycle and electron transport chain inhibitors were each tested and compared to zero palmitate control incubations. Paired comparisons of the 0 and 20 min samples were made by Student's t-test. False discovery rate were estimated and Type I error rates assigned. Major metabolite groups were organic acids, amines and amino acids, free fatty acids and sugar phosphates. Palmitate oxidation was associated with unique profiles of metabolites, a subset of which correlated to palmitate oxidation rate. In particular, palmitate oxidation rate was associated with distinct changes in the levels of TCA cycle intermediates within and effluxed from mitochondria. Conclusions/Significance: This proof-of-principle study establishes that large-scale metabolomics methods can be applied to organelle-level models to discover metabolite patterns reflective of LCFA combustion, which may lead to identification of molecules linking muscle fat metabolism and insulin signaling. Our results suggest that future studies should focus on the fate of effluxed TCA cycle intermediates and on mechanisms ensuring their replenishment during LCFA metabolism in skeletal muscle. C1 [Seifert, Erin L.; Bezaire, Veronic; Harper, Mary-Ellen] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada. [Fiehn, Oliver; Wohlgemuth, Gert] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Bickel, David R.] Univ Ottawa, Ottawa Inst Syst Biol, Ottawa, ON, Canada. [Adams, Sean H.] Univ Calif Davis, ARS, Obes & Metab Res Unit, USDA,Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Seifert, EL (reprint author), Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada. EM sean.h.adams@ars.usda.gov; Maryellen.Harper@uottawa.ca RI Bickel, David/F-9370-2010; Harper, Mary-Ellen/C-3103-2009; OI Bickel, David/0000-0002-9623-3946; Harper, Mary-Ellen/0000-0003-3864-5886 FU United States Department of Agriculture Agricultural Research Service (USDA-ARS) [530651530-016-00D]; National Institutes of Health National Institute of Diabetes and Digestive and Kidney Diseases (NIH-NIDDK) [R01DK078328]; Canadian Institutes for Health Research [57810]; Canadian Diabetes Association FX This work was supported in part through intramural United States Department of Agriculture Agricultural Research Service (USDA-ARS) Project 530651530-016-00D (S. H. A.) and National Institutes of Health National Institute of Diabetes and Digestive and Kidney Diseases (NIH-NIDDK) R01DK078328 (S. H. A. and M-E. H.) and by the Canadian Institutes for Health Research (Nutrition, Metabolism and Diabetes (57810); M-E. H.). E. L. S. was the recipient of a fellowship from the Canadian Diabetes Association. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 19 Z9 20 U1 1 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 24 PY 2010 VL 5 IS 3 AR e9834 DI 10.1371/journal.pone.0009834 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 573FT UT WOS:000275894500008 PM 20352092 ER PT J AU Vincent, AL Ciacci-Zanella, JR Lorusso, A Gauger, PC Zanella, EL Kehrli, ME Janke, BH Lager, KM AF Vincent, Amy L. Ciacci-Zanella, Janice R. Lorusso, Alessio Gauger, Philip C. Zanella, Eraldo L. Kehrli, Marcus E., Jr. Janke, Bruce H. Lager, Kelly M. TI Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs SO VACCINE LA English DT Article DE A/H1N1 2009; Swine; Influenza A virus; Vaccine ID UNITED-STATES; A VIRUSES; INFECTION; HUMANS; VACCINATION; ORIGINS AB The gene constellation of the 2009 pandemic A/H1N1 virus is a unique combination from swine influenza A viruses (SIV) of North American and Eurasian lineages, but prior to April 2009 had never before been identified in swine or other species. Although its hemagglutinin gene is related to North American H1 SW, it is unknown if vaccines currently used in U.S. swine would cross-protect against infection with the pandemic A/H1N1. The objective of this study was to evaluate the efficacy of inactivated vaccines prepared with North American swine influenza viruses as well as an experimental homologous A/H1N1 vaccine to prevent infection and disease from 2009 pandemic A/H1N1. All vaccines tested provided partial protection ranging from reduction of pneumonia lesions to significant reduction in virus replication in the lung and nose. The multivalent vaccines demonstrated partial protection; however, none was able to prevent all nasal shedding or clinical disease. An experimental homologous 2009 A/H1N1 monovalent vaccine provided optimal protection with no virus detected from nose or lung at any time point in addition to amelioration of clinical disease. Based on cross-protection demonstrated with the vaccines evaluated in this study, the U.S. swine herd likely has significant immunity to the 2009 A/H1N1 from prior vaccination or natural exposure. However, consideration should be given for development of monovalent homologous vaccines to best protect the swine population thus limiting shedding and the potential transmission of 2009 A/H1N1 from pigs to people. Published by Elsevier Ltd. C1 [Vincent, Amy L.; Ciacci-Zanella, Janice R.; Lorusso, Alessio; Zanella, Eraldo L.; Kehrli, Marcus E., Jr.; Lager, Kelly M.] USDA ARS, Natl Anim Dis Ctr, Virus & Pr Dis Livestock Res Unit, Ames, IA 50010 USA. [Ciacci-Zanella, Janice R.] Brazilian Agr Res Corp, EMBRAPA, Labex USA, Brasilia, DF, Brazil. [Gauger, Philip C.; Janke, Bruce H.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Zanella, Eraldo L.] Univ Passo Fundo, Fac Agron & Med Vet, Passo Fundo, RS, Brazil. RP Vincent, AL (reprint author), USDA ARS, Natl Anim Dis Ctr, Virus & Pr Dis Res Unit, 1920 Dayton Rd, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov RI Zanella, Janice/C-3632-2014; Lorusso, Alessio/A-7311-2016; OI Lorusso, Alessio/0000-0001-7933-7367; Lorusso, Alessio/0000-0001-6156-8212 FU USDA-ARS FX The authors thank Michelle Harland and Hillary Horst for technical assistance and Brian Pottebaum, Jason Huegel, and Dr. Becky Jepsen for assistance with animal studies. Assistance with microbiologic screening from Dr. Susan Brockmeier is appreciated. We thank Dr. Alexander Klimov and Ruben Donis (CDC) for providing pandemic H1N1 isolates. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Funding was provided by USDA-ARS. NR 26 TC 45 Z9 47 U1 0 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 24 PY 2010 VL 28 IS 15 BP 2782 EP 2787 DI 10.1016/j.vaccine.2010.01.049 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 576UK UT WOS:000276174600015 PM 20132919 ER PT J AU Antunez, M Anido, M Evans, JD Zunino, P AF Antunez, Marina Anido, Matilde Evans, Jay D. Zunino, Pablo TI Secreted and immunogenic proteins produced by the honeybee bacterial pathogen, Paenibacillus larvae SO VETERINARY MICROBIOLOGY LA English DT Article DE Paenibacillus larvae; American Foulbrood; Secretome; Pathogenicity; Virulence factors ID BACILLUS-ANTHRACIS SECRETOME; ALPHA-ENOLASE; AMERICAN FOULBROOD; SIGNAL PEPTIDES; BINDING-PROTEIN; SURFACE; IDENTIFICATION; THURINGIENSIS; CEREUS; MODEL AB American Foulbrood is a severe disease affecting larvae of honeybee Apis mellifera, causing significant decrease in the honeybee population, beekeeping industries and agricultural production In spite of its importance, little is known about the virulence factors secreted by Paenibacillus larvae during larval infection. The aim of the present work was to perform a first approach to the identification and characterization of P larvae secretome. P. larvae secreted proteins were analyzed by SDS-PAGE and identified by MALDI-TOF. Protein toxicity was evaluated using an experimental model based on feeding of A mellifera larvae and immunogenicity was evaluated by Western blot, using an antiserum raised against cells and spores of P larvae. Ten different proteins were identified among P larvae secreted proteins, including proteins involved in transcription, metabolism, translation, cell envelope, transport, protein folding, degradation of polysaccharides and motility Although most of these proteins are cytosolic, many of them have been previously detected in the extracellular medium of different Bacillus spp. cultures and have been related to virulence. The secreted proteins resulted highly toxic and immunogenic when larvae were exposed using an experimental model. This is the first description of proteins secreted by the honeybee pathogen P larvae This information may be relevant for the elucidation of bacterial pathogenesis mechanisms (C) 2009 Elsevier B.V. All rights reserved C1 [Antunez, Marina; Anido, Matilde; Zunino, Pablo] Inst Invest Biol Clemente Estable, Dept Microbiol, Montevideo 11600, Uruguay. [Evans, Jay D.] ARS, USDA, Bee Res Lab, Beltsville, MD USA. RP Antunez, M (reprint author), Inst Invest Biol Clemente Estable, Dept Microbiol, Avda Italia 3318, Montevideo 11600, Uruguay. RI Evans, Jay/C-8408-2012; OI Evans, Jay/0000-0002-0036-4651; Antunez, Karina/0000-0002-3664-9835 FU PDT [63/112]; PEDECIBA (Uruguay); USDA-ARS (USA) FX Finding support from PDT 63/112 and PEDECIBA (Uruguay) and USDA-ARS (USA). We thank Dawn Lopez for field and laboratory assistance. NR 36 TC 0 Z9 0 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD MAR 24 PY 2010 VL 141 IS 3-4 BP 385 EP 389 DI 10.1016/j.vetmic.2009.09.006 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 576HR UT WOS:000276135600025 ER PT J AU Hoffmann, M Brown, EW Feng, PCH Keys, CE Fischer, M Monday, SR AF Hoffmann, Maria Brown, Eric W. Feng, Peter C. H. Keys, Christine E. Fischer, Markus Monday, Steven R. TI PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species SO BMC MICROBIOLOGY LA English DT Article ID SP NOV.; MOLECULAR-IDENTIFICATION; PARAHAEMOLYTICUS STRAINS; GENUS VIBRIO; COMB. NOV; SEQUENCES; RECLASSIFICATION; HETERODUPLEXES; POLYMORPHISMS; AMPLIFICATION AB Background: The genus Vibrio is a diverse group of Gram-negative bacteria comprised of 74 species. Furthermore, the genus has and is expected to continue expanding with the addition of several new species annually. Consequently, it is of paramount importance to have a method which is able to reliably and efficiently differentiate the numerous Vibrio species. Results: In this study, a novel and rapid polymerase chain reaction (PCR)-based intergenic spacer (IGS)-typing system for vibrios was developed that is based on the well-known IGS regions located between the 16S and 23S rRNA genes on the bacterial chromosome. The system was optimized to resolve heteroduplex formation as well as to take advantage of capillary gel electrophoresis technology such that reproducible analyses could be achieved in a rapid manner. System validation was achieved through testing of 69 archetypal Vibrio strains, representing 48 Vibrio species, from which an 'IGS-type' profile database was generated. These data, presented here in several cluster analyses, demonstrated successful differentiation of the 69 type strains showing that this PCR-based fingerprinting method easily discriminates bacterial strains at the species level among Vibrio. Furthermore, testing 36 strains each of V. parahaemolyticus and V. vulnificus, important food borne pathogens, isolated from a variety of geographical locations with the IGS-typing method demonstrated distinct IGS-typing patterns indicative of subspecies divergence in both populations making this technique equally useful for intraspecies differentiation, as well. Conclusion: This rapid, reliable and efficient IGS-typing system, especially in combination with 16S rRNA gene sequencing, has the capacity to not only discern and identify vibrios at the species level but, in some cases, at the sub-species level, as well. This procedure is particularly well-suited for preliminary species identification and, lends itself nicely to epidemiological investigations providing information more quickly than other time-honoured methods traditionally used in these types of analyses. C1 [Hoffmann, Maria; Brown, Eric W.; Feng, Peter C. H.; Keys, Christine E.; Monday, Steven R.] USDA, Off Regulatory Sci Ctr Food Safety & Appl Nutr, Div Microbiol, College Pk, MD 20740 USA. [Hoffmann, Maria; Fischer, Markus] Univ Hamburg, Inst Food Chem, D-20146 Hamburg, Germany. RP Hoffmann, M (reprint author), USDA, Off Regulatory Sci Ctr Food Safety & Appl Nutr, Div Microbiol, Paint Branch Pkwy, College Pk, MD 20740 USA. EM maria.hoffman@fda.hhs.gov RI Fischer, Markus/G-9477-2012 FU Research Fellowship Program FX This project was supported by an appointment of MH to the Research Fellowship Program for the Center for Food Safety and Applied Nutrition administered by the Oak Ridge Associated Universities. The authors wish to thank Dr. Gonz~lez- Escalona for sharing his V. vulnificus and V. parahaemolyticus strains and for his insights in this study. NR 31 TC 19 Z9 21 U1 2 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD MAR 23 PY 2010 VL 10 AR 90 DI 10.1186/1471-2180-10-90 PG 14 WC Microbiology SC Microbiology GA 585SW UT WOS:000276850300002 PM 20331883 ER PT J AU Bae, S Ro, KS Flora, JRV Novak, J Berge, ND AF Bae, Sunyoung Ro, Kyoung S. Flora, Joseph R. V. Novak, Jeff Berge, Nicole D. TI Changes in physicochemical characteristics of biochars by hydrothermal and dry carbonization of swine solids and poultry litter SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 N Carolina Agr & Tech State Univ, Dept Chem, Greensboro, NC USA. N Carolina Agr & Tech State Univ, Energy & Environm Syst Program, Greensboro, NC USA. ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, Florence, SC USA. Univ S Carolina, Dept Civil & Environm Engn, Columbia, SC 29208 USA. NR 0 TC 0 Z9 0 U1 5 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 31-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189302315 ER PT J AU Bilbao-Sainz, C Avena-Bustillos, RJ Wood, DF Williams, TG McHugh, TH AF Bilbao-Sainz, Cristina Avena-Bustillos, Roberto J. Wood, Delilah F. Williams, Tina G. McHugh, Tara H. TI Nano-emulsions prepared by a low-energy emulsification method applied to edible films SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Bilbao-Sainz, Cristina; Avena-Bustillos, Roberto J.; Wood, Delilah F.; Williams, Tina G.; McHugh, Tara H.] ARS, WRRC, USDA, Albany, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 248-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300067 ER PT J AU Bozell, JJ Sanyal, N Elder, T AF Bozell, Joseph J. Sanyal, Nibedita Elder, Thomas TI Effect of headgroup structure and chain length on self-assembly of glycal-based bolaamphiphiles SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Tennessee, Forest Prod Ctr, Knoxville, TN USA. US Forest Serv, So Res Stn, USDA, Pineville, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 567-ORGN PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189303884 ER PT J AU Britz, SJ AF Britz, Steven J. TI Deep ultraviolet light-emitting diodes (DUV-LEDs): A novel approach to determine wavelength response curves SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Britz, Steven J.] USDA, Food Components & Hlth Lab, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 153-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300022 ER PT J AU Brown, CL Callcott, AM AF Brown, Charles L. Callcott, Anne-Marie TI Pesticides and the federal imported fire ant quarantine SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 USDA APHIS PPQ Emergency Domest Programs, Riverdale, MD USA. USDA APHIS PPQ Ctr Plant Hlth Sci & Technol, Gulfport, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 20-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300151 ER PT J AU Cabrera, A Canamero, A Velarde, P Cox, L Cornejo, J Koskinen, WC AF Cabrera, Alegria Canamero, Anabel Velarde, Pilar Cox, Lucia Cornejo, Juan Koskinen, William C. TI Effect of organic waste soil amendments on fate of MCPA in agricultural soils SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Minnesota, Dept Soil Water & Climate, St Paul, MN USA. IRNAS CSIC, Dept AgroquAm & ConservaciA3n Suelos, Seville, Spain. USDA ARS, St Paul, MN USA. RI COX, LUCIA/M-4072-2015 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 191-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300146 ER PT J AU Chiou, BS Avena-Bustillos, R Bechtel, P Imam, S Glenn, G Orts, W AF Chiou, Bor-Sen Avena-Bustillos, Roberto Bechtel, Peter Imam, Syed Glenn, Greg Orts, William TI Effects of helical content on water vapor barrier properties of fish gelatin films SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 USDA, Albany, CA USA. USDA, SARU, Fairbanks, AK USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 19-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300038 ER PT J AU Davis, PA Vasu, V Gohil, K Kim, H Yokoyama, W Khan, I Cross, C AF Davis, Paul Andrew Vasu, Vihas Gohil, Kishore Kim, Hyunsook Yokoyama, Wallace Khan, Imran Cross, Carroll TI Walnuts reduce prostate tumor size and growth in a mouse model of prostate cancer SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA. ARS, USDA, WRRC, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 59-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300085 ER PT J AU Dayan, F AF Dayan, Franck TI Natural products in weed management: A review SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Dayan, Franck] USDA ARS, Nat Prod Utilizat Res Unit, University, MS USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 117-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300009 ER PT J AU Del Grosso, S AF Del Grosso, Stephen TI Soil nitrogen cycling and GHG accounting methodologies SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Del Grosso, Stephen] USDA, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 55-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300231 ER PT J AU Doratt, RE Pitt, WC AF Doratt, Rogelio E. Pitt, William C. TI Chemical management for controlling invasive frogs in Hawaii SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Doratt, Rogelio E.; Pitt, William C.] USDA, Natl Wildlife Res Ctr, Hilo, HI USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 46-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300229 ER PT J AU Eggleston, G Gober, J Alexander, C AF Eggleston, Gillian Gober, Jessica Alexander, Clay TI Analysis of mannitol as a deterioration marker in sugarcane and sugar beet factories SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Eggleston, Gillian; Gober, Jessica; Alexander, Clay] ARS, USDA, SRRC, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 20-CARB PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300713 ER PT J AU Elder, T AF Elder, Thomas TI Application of computational chemistry to lignin pyrolysis SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Elder, Thomas] US Forest Serv, USDA, So Res Stn, Pineville, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 148-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300863 ER PT J AU Fagerquist, CK AF Fagerquist, Clifton K. TI Rapid identification of protein biomarkers and toxins from food-borne pathogens by top-down proteomics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Fagerquist, Clifton K.] Agr Res Serv, USDA, Western Reg Res Ctr, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 12-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300111 ER PT J AU Fan, ZL Wu, WH Zhang, RF Kasuga, T Xiong, XC AF Fan, Zhiliang Wu, Weihua Zhang, Ruifu Kasuga, Takao Xiong, Xiaochao TI Improvement of the reactive intermediate production for a novel biochemical platform for fuels and chemicals production from cellulosic biomass SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. USDA ARS, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 121-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300012 ER PT J AU Finkenstadt, VL Bucur, C Evans, KO Cote, GL Willett, JL AF Finkenstadt, Victoria L. Bucur, Claudiu Evans, Kervin O. Cote, Gregory L. Willett, J. L. TI Bacterial exopolysaccharides for corrosion inhibition on metal substrates SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Finkenstadt, Victoria L.; Bucur, Claudiu; Evans, Kervin O.; Cote, Gregory L.; Willett, J. L.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 210-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300049 ER PT J AU Guraya, HS Lima, IM Champagne, E AF Guraya, Harmeet S. Lima, Isabel M. Champagne, Elaine TI Method of creating starch-like ultra-fine rice flour and effect of spray drying on formation of free fatty acid SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Guraya, Harmeet S.; Lima, Isabel M.; Champagne, Elaine] ARS, Food Proc Sensory Qual Unit, USDA, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 131-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300016 ER PT J AU Hammel, KE Yelle, DJ Ralph, J Wei, DS Kapich, AN AF Hammel, Kenneth E. Yelle, Daniel J. Ralph, John Wei, Dongsheng Kapich, Alexander N. TI Solution-state NMR analysis of dissolved, brown-rotted wood reveals that the responsible fungi are ligninolytic SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 USDA, Forest Prod Lab, Madison, WI 53705 USA. Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 292-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189301034 ER PT J AU Hawkins, S Amini, PJ Chen, GJ Xu, BQ AF Hawkins, Samantha Amini, P. J. Chen, Guojun Xu, Bingqian TI Characterization of novel surfaces by FTIR spectroscopy and Atomic Force Microscopy for food pathogen detection SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, Athens, GA USA. Washington Univ, St Louis, MO USA. Univ Georgia, Athens, GA 30602 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 225-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300057 ER PT J AU Hoh, E Lehotay, SJ Pangallo, KC Reddy, CM AF Hoh, Eunha Lehotay, Steven J. Pangallo, Kristin C. Reddy, Christopher M. TI Untargeted analysis of organic contaminants in dolphin blubber using DSI-GC(A)over-tilde-GC/TOF-MS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 San Diego State Univ, Grad Sch Publ Hlth, Div Environm Hlth, San Diego, CA 92182 USA. ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 285-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300186 ER PT J AU Holtman, KM Franqui, D Bozzi, DV Orts, WJ AF Holtman, Kevin M. Franqui, Diana Bozzi, David V. Orts, William J. TI Vacuum assisted steam autoclaving as a front-end technology for converting waste to bioenergy SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Holtman, Kevin M.; Franqui, Diana; Bozzi, David V.; Orts, William J.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 179-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189302245 ER PT J AU Huang, YW Zhao, YP Park, B AF Huang, Yao-wen Zhao, Yiping Park, Bosoon TI Detection of pathogens and toxins by surface enhanced Raman spectroscopy using silver nanorods array substrates SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. Univ Georgia, Dept Phys & Astron, Athens, GA 30602 USA. ARS, USDA, Athens, GA USA. RI Zhao, Yiping/A-4968-2008 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 226-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300058 ER PT J AU Karaaslan, AM Buschle-Diller, G Tshabalala, M AF Karaaslan, Ahmet M. Buschle-Diller, Gisela Tshabalala, Mandla TI Biocompatible hydrogels reinforced with modified cellulose nanocrystals SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Auburn Univ, Dept Polymer & Fiber Engn, Auburn, AL 36849 USA. US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 49-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189301064 ER PT J AU Krainz, K Suess, HU Dietz, T Mereiter, K French, A Potthast, A Rosenau, T AF Krainz, Karin Suess, Hans Ulrich Dietz, Thomas Mereiter, Kurt French, Alfred Potthast, Antje Rosenau, Thomas TI Mechanistic studies on aging and chromophore formation in celluloses SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 BOKU Univ, Dept Chem, Vienna, Austria. Degussa Evonik, Hanau, Germany. Vienna Univ Technol, Fac Chem, A-1040 Vienna, Austria. USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 179-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300875 ER PT J AU Lance, DR AF Lance, David R. TI A chemical history of gypsy moth management in North Americ SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Lance, David R.] USDA APHIS, Dept Plant Protect & Quarantine, Buzzards Bay, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 21-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300158 ER PT J AU Lentz, RD Ippolito, J AF Lentz, Rodrick D. Ippolito, Jim TI Biochar, manure, or sawdust additions to calcareous subsoils: Effects on nutrient availability and carbon storage SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Lentz, Rodrick D.; Ippolito, Jim] ARS, USDA, NWISRL, Kimberly, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 36-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189302343 ER PT J AU Lima, IM Ro, KS Boateng, AA Klasson, KT AF Lima, Isabel M. Ro, Kyoung S. Boateng, Akwasi A. Klasson, Kjell T. TI Biochars from agricultural residuals as adsorbents for environmental remediation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. ARS, USDA, So Reg Res Ctr, Wyndmoor, PA USA. ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 32-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189302320 ER PT J AU Lin, CH Thompson, BM Hsieh, HY Lerch, RN Gold, MA Garrett, HE Kremer, RJ AF Lin, Chung-Ho Thompson, Brian M. Hsieh, Hsin-Yeh Lerch, Robert N. Gold, Michael A. Garrett, Harold E. Kremer, Robert J. TI Screening and isolating phytochemicals in Eastern Redcedar (Juniperus virginiana) for developing potential entrepreneurial opportunities SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Missouri, Ctr Agroforestry, Columbia, MO USA. Univ Missouri, Dept Forestry, Columbia, MO USA. Univ Missouri, Coll Vet Med, Dept Vet Pathobiol, Columbia, MO USA. Agr Res Serv, Cropping & Water Qual Res Unit, USDA, Columbia, MO USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 101-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300104 ER PT J AU Lin, JT AF Lin, Jiann-Tsyh TI Identification of trihydroxy fatty acids in castor oil and the regiospecific quantification of the triacylglycerols by mass spectrometry SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Lin, Jiann-Tsyh] USDA, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 207-ANYL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300306 ER PT J AU Lingle, S AF Lingle, Sarah TI Opportunities and challenges of sweet sorghum as a feedstock for biofuel SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Lingle, Sarah] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 74-CARB PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300732 ER PT J AU McKeon, TA AF McKeon, Thomas A. TI Industrial applications of castor oil and the castor plant SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [McKeon, Thomas A.] ARS, USDA, WRRC, Albany, CA USA. NR 0 TC 0 Z9 0 U1 3 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 244-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300066 ER PT J AU McMahan, C Boateng, AA Cornish, K AF McMahan, Colleen Boateng, Akwasi A. Cornish, Katrina TI Fast pyrolysis of guayule biomass SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, WRRC, Albany, CA USA. ARS, USDA, ERRC, Wyndmoor, PA USA. Yulex Corp, Maricopa, AZ USA. RI Cornish, Katrina/A-9773-2013 NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 144-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300125 ER PT J AU Meepagala, KM Osbrink, W Burandt, C Lax, AR AF Meepagala, Kumudini M. Osbrink, Weste Burandt, Charles Lax, Alan R. TI Natural product based chromenes as a novel class of potential termiticide SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, NPURU, University, MS USA. ARS, USDA, SRRC, New Orleans, LA USA. Natl Ctr Nat Prod, University, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 105-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300105 ER PT J AU Mizuno, CS Rimando, AM Duke, SO AF Mizuno, Cassia S. Rimando, Agnes M. Duke, Stephen O. TI Herbicidal activity of sorgoleone analogs SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Mizuno, Cassia S.; Rimando, Agnes M.; Duke, Stephen O.] USDA, University, MS USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 92-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300249 ER PT J AU Mullin, CA Frazier, M Frazier, JL Ashcraft, S Simonds, R vanEngelsdorp, D Pettis, JS AF Mullin, Christopher A. Frazier, Maryann Frazier, James L. Ashcraft, Sara Simonds, Roger vanEngelsdorp, Dennis Pettis, Jeffery S. TI High levels of miticides and systemic agrochemicals in North American beehives: Implications for honey bee health SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. USDA AMS Natl Sci Lab, Gastonia, NC USA. Penn Dept Agr, Harrisburg, PA USA. USDA ARS, Bee Res Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 4 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 338-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300214 ER PT J AU Pearce, EM Brown, CH Cheng, HN AF Pearce, Eli M. Brown, Cheryl H. Cheng, H. N. TI Senior Chemists Task Force: An Update SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NYU, Polytech Inst, Dept Chem & Biol Sci, Brooklyn, NY USA. Amer Chem Soc, Off Local Sect Act, Washington, DC 20036 USA. USDA ARS, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 8-PROF PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189304873 ER PT J AU Place, SE Hamilton, SW DePeters, EJ McGarvey, JA Lathrop, J Mitloehner, FM AF Place, Sara E. Hamilton, Scott W. DePeters, Edward J. McGarvey, Jeffery A. Lathrop, Jeremy Mitloehner, Frank M. TI Effects of dietary monensin on greenhouse gases from lactating dairy cows and waste SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. ARS, Plant Mycotoxin Res Unit, USDA, Albany, CA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 85-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300245 ER PT J AU Rimando, AM Fiore, M Evidente, A AF Rimando, Agnes M. Fiore, Michele Evidente, Antonio TI Analysis of phytotoxic fungal toxins from Ascochyta caulina liquid culture SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, University, MS USA. Univ Naples Federico 2, Dipartimento Sci Suolo Pianta Ambiente & Prod Ani, Portici, Italy. Siena Biotech, Siena, Italy. RI Evidente, Antonio/N-9357-2013 OI Evidente, Antonio/0000-0001-9110-1656 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 96-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300251 ER PT J AU Silva, CJ Onisko, BC Dynin, I Erickson, ML Carter, JM AF Silva, Christopher J. Onisko, Bruce C. Dynin, Irina Erickson, Melissa L. Carter, John Mark TI Detecting prions: Discriminating isoforms in the attomole range SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, USDA, Albany, CA USA. OniPro Biosci, Kensington, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 40-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300226 ER PT J AU Stevens, ED Sasabayashi, R Dowd, MK Johnson, GP French, AD AF Stevens, Edwin D. Sasabayashi, Ryuta Dowd, Michael K. Johnson, Glenn P. French, Alfred D. TI X-ray diffraction and quantum mechanical studies of the electron density distribution in methyl-b-cellobioside SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. ARS, Commod Utilizat Res Unit, So Reg Res Ctr, USDA, New Orleans, LA USA. ARS, Cotton Struct & Qual Res Unit, So Reg Res Ctr, USDA, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 145-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300862 ER PT J AU Sun, L Li, CL Chuck, G Dibble, DC Simmons, BA Singh, S AF Sun, Lan Li, Chenlin Chuck, George Dibble, Dean C. Simmons, Blake A. Singh, Seema TI Genetic enhancement of bioenergy feedstocks for efficient biofuel production SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Joint BioEnergy Inst, Dept Deconstruct, Emeryville, CA USA. Sandia Natl Labs, Dept Biomass Sci & Convers Technol, Livermore, CA USA. USDA, Dept Agr Res Serv, Albany, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 266-CELL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189301025 ER PT J AU Thompson, BM Lin, CH Hsieh, HY Lerch, RN Kremer, RJ Garrett, HE AF Thompson, Brian M. Lin, Chung-Ho Hsieh, Hsin-Yeh Lerch, Robert N. Kremer, Robert J. Garrett, Harold E. TI Detection and quantification of atzA in rhizosphere soil and enhanced bioremediation of atrazine by Pseudomonas sp. strain ADP SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Missouri, Coll Vet Med, Dept Vet Pathobiol, Columbia, MO USA. Univ Missouri, Ctr Agroforestry, Columbia, MO USA. Univ Missouri, Dept Forestry, Columbia, MO USA. USDA ARS, Cropping & Water Qual Res Unit, Columbia, MO USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 193-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300147 ER PT J AU Venterea, RT AF Venterea, Rodney T. TI Urea fertilizer decreases N2O emissions by 50% compared with anhydrous ammonia in corn/soybean cropping systems in Minnesota SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 [Venterea, Rodney T.] USDA ARS, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 52-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300230 ER PT J AU Xu, LY Yu, Y Zhu, HP Ozkan, E AF Xu, Linyun Yu, Yang Zhu, Heping Ozkan, Erdal TI Influence of target surfaces and adjuvants on deposition characteristics of spray droplets SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Nanjing Forestry Univ, Nanjing, Jiangsu, Peoples R China. Yunnan Agr Univ, Kunming City, Peoples R China. Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Applicat Technol Res Unit, Wooster, OH 44691 USA. Ohio State Univ, Columbus, OH 43210 USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 249-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300170 ER PT J AU Xuan, RC Yates, SR AF Xuan, Richeng Yates, Scott R. TI Steroid hormones degradation in active slurry solution SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 USDA ARS, US Salin Lab, Riverside, CA 92501 USA. Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 361-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189302344 ER PT J AU Yokoyama, WH Kim, H Bartley, GE Young, SA Anderson, WHK Hung, SC Albers, DR Langhorst, ML Turowski, M AF Yokoyama, Wallace H. Kim, Hyunsook Bartley, Glenn E. Young, Scott A. Anderson, W. H. Kerr Hung, Shao-Ching Albers, David R. Langhorst, Marsha L. Turowski, Maciej TI Plasma cholesterol and changes in hepatic genes regulating cholesterol metabolism by HPMC in hamsters SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 ARS, Western Reg Res Ctr, USDA, Albany, CA USA. Dow Wolff Cellulos, Midland, MI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 2010 VL 239 MA 34-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA V21DW UT WOS:000208189300080 ER PT J AU Birdsey, RA Lucas, R Pan, YD Sun, G Gustafson, EJ Perera, AH AF Birdsey, Richard A. Lucas, Richard Pan, Yude Sun, Ge Gustafson, Eric J. Perera, Ajith H. TI Managing landscapes at multiple scales for sustainability of ecosystem functions Preface SO FOREST ECOLOGY AND MANAGEMENT LA English DT Editorial Material C1 [Birdsey, Richard A.; Pan, Yude] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. [Lucas, Richard] Univ Wales, Aberystwyth SY23 3DB, Ceredigion, Wales. [Sun, Ge] US Forest Serv, USDA, So Res Stn, Raleigh, NC 27606 USA. [Gustafson, Eric J.] US Forest Serv, USDA, Rhinelander, WI 54501 USA. [Perera, Ajith H.] Ontario Minist Nat Resources, Sault Ste Marie, ON P6A 2E5, Canada. RP Birdsey, RA (reprint author), US Forest Serv, USDA, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM rbirdsey@fs.fed.us; rml@aber.ac.uk; ypan@fs.fed.us; ge_sun@ncsu.edu; egustafson@fs.fed.us; ajith.perera@ontario.ca NR 0 TC 0 Z9 0 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1213 EP 1214 DI 10.1016/j.foreco.2010.01.022 PG 2 WC Forestry SC Forestry GA 572ES UT WOS:000275810900001 ER PT J AU Guo, ZD Fang, JY Pan, YD Birdsey, R AF Guo, Zhaodi Fang, Jingyun Pan, Yude Birdsey, Richard TI Inventory-based estimates of forest biomass carbon stocks in China: A comparison of three methods SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article; Proceedings Paper CT Conference of the Landscape-Ecology-Working-Group of the International-Union-of-Forest-Research-Organizations CY SEP 16-22, 2008 CL Chengdu, PEOPLES R CHINA SP Int Union Forest Res Org, Landscape Ecol Working Grp, USDA Forest Serv DE China; Biomass expansion factor (BEF); Forest age class; Forest biomass; Forest type; Mean biomass ID TROPICAL FORESTS; UNITED-STATES; STORAGE; BALANCE; VEGETATION; TEMPERATE; BUDGET; SINKS; POOLS AB Several studies have reported different estimates for forest biomass carbon (C) stocks in China. The discrepancy among these estimates may be largely attributed to the methods used. In this study, we used three methods [mean biomass density method (MBM), mean ratio method (MRM), and continuous biomass expansion factor (BEF) method (abbreviated as CBM)] applied to forest inventory data to estimate China's forest biomass C stocks and their changes from 1984 to 2003. The three methods generated various estimates of the biomass C stocks: the lowest (4.0-5.9 Pg C) from CBM and the highest (5.7-7.7 Pg C) from MBM, with an intermediate estimate (4.2-6.2 Pg C) from MRM. Forest age class is a major factor responsible for these method-induced differences. MBM overestimates biomass for young-aged forests, but underestimates biomass for old-aged forests; while the reverse is true for MRM. Further, the three methods resulted in different estimates of biomass C stocks for different forest types. For temperate/subtropical mixed forests, MBM generated a 92% higher estimate than CBM and MRM generated a 14% lower than CBM. The degree of the overestimates is closely related with the proportion of young-aged forest within total area of each forest type. (C) 2009 Elsevier B.V. All rights reserved. C1 [Guo, Zhaodi; Fang, Jingyun] Peking Univ, Coll Urban & Environm Sci, Minist Educ, Dept Ecol, Beijing 100871, Peoples R China. [Guo, Zhaodi; Fang, Jingyun] Peking Univ, Coll Urban & Environm Sci, Minist Educ, Key Lab Earth Surface Proc, Beijing 100871, Peoples R China. [Pan, Yude; Birdsey, Richard] US Forest Serv, USDA, Global Change Program, Newtown Sq, PA 19073 USA. RP Fang, JY (reprint author), Peking Univ, Coll Urban & Environm Sci, Minist Educ, Dept Ecol, Beijing 100871, Peoples R China. EM jyfang@urban.pku.edu.cn RI Pan, Yude/F-6145-2015 NR 51 TC 66 Z9 84 U1 4 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1225 EP 1231 DI 10.1016/j.foreco.2009.09.047 PG 7 WC Forestry SC Forestry GA 572ES UT WOS:000275810900003 ER PT J AU Bradford, JB Weishampel, P Smith, ML Kolka, R Birdsey, RA Ollinger, SV Ryan, MG AF Bradford, John B. Weishampel, Peter Smith, Marie-Louise Kolka, Randall Birdsey, Richard A. Ollinger, Scott V. Ryan, Michael G. TI Carbon pools and fluxes in small temperate forest landscapes: Variability and implications for sampling design SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article; Proceedings Paper CT Conference of the Landscape-Ecology-Working-Group of the International-Union-of-Forest-Research-Organizations CY SEP 16-22, 2008 CL Chengdu, PEOPLES R CHINA SP Int Union Forest Res Org, Landscape Ecol Working Grp, USDA Forest Serv DE Terrestrial carbon cycling; Net ecosystem carbon balance; Spatial patterns; Sample size; Biometric carbon measurements ID NET PRIMARY PRODUCTION; GROSS PRIMARY PRODUCTION; LODGEPOLE PINE FORESTS; PRIMARY PRODUCTION NPP; BELOW-GROUND BIOMASS; DECIDUOUS FOREST; SPATIAL VARIABILITY; NORTHERN WISCONSIN; INTERSECT METHOD; REGIONAL-SCALE AB Assessing forest carbon storage and cycling over large areas is a growing challenge that is complicated by the inherent heterogeneity of forest systems. Field measurements must be conducted and analyzed appropriately to generate precise estimates at scales large enough for mapping or comparison with remote sensing data. In this study we examined spatial variability in three small temperate forest landscapes. Our objectives were (1) to quantify the magnitude and scale of variability in stand structure, carbon pools and carbon fluxes and (2) to assess how this variability influences both optimal sampling strategy and required sampling intensity. Stand structure was consistently less variable than carbon pools or fluxes, suggesting that measuring carbon dynamics may require more intense sampling than traditional forestry inventories. Likewise, the magnitude of variability differed substantially among response variables, implying that sampling efficiency can be enhanced by adopting a flexible sampling strategy that is optimized for each carbon pool. Our results indicate that plots dispersed across the study area are generally more effective than clustered plots for characterizing carbon dynamics. Published by Elsevier B.V. C1 [Bradford, John B.; Kolka, Randall] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Weishampel, Peter] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Smith, Marie-Louise] US Forest Serv, USDA, Washington, DC 20250 USA. [Birdsey, Richard A.] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. [Ollinger, Scott V.] Univ New Hampshire, Complex Syst Res Ctr, Inst Study Earth Oceans & Space, Durham, NH 03820 USA. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Ryan, Michael G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. RP Bradford, JB (reprint author), US Forest Serv, USDA, No Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. EM jbbradford@fs.fed.us RI Ryan, Michael/A-9805-2008; Bradford, John/E-5545-2011; Ollinger, Scott/N-3380-2014 OI Ryan, Michael/0000-0002-2500-6738; Ollinger, Scott/0000-0001-6226-1431 NR 99 TC 22 Z9 24 U1 0 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1245 EP 1254 DI 10.1016/j.foreco.2009.04.009 PG 10 WC Forestry SC Forestry GA 572ES UT WOS:000275810900006 ER PT J AU Johnson, K Scatena, FN Pan, YD AF Johnson, Kristofer Scatena, Frederick N. Pan, Yude TI Short- and long-term responses of total soil organic carbon to harvesting in a northern hardwood forest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article; Proceedings Paper CT Conference of the Landscape-Ecology-Working-Group of the International-Union-of-Forest-Research-Organizations CY SEP 16-22, 2008 CL Chengdu, PEOPLES R CHINA SP Int Union Forest Res Org, Landscape Ecol Working Grp, USDA Forest Serv DE Northern hardwood forest; Soil carbon modeling; Process-based modeling; Forest soil carbon ID LUQUILLO EXPERIMENTAL FOREST; OLD-GROWTH; SITE PRODUCTIVITY; MATTER DYNAMICS; PUERTO-RICO; MODEL; SCALE; SEQUESTRATION; SIMULATION; BIOMASS AB The long-term response of total soil organic carbon pools ('total SOC', i.e. soil and dead wood) to different harvesting scenarios in even-aged northern hardwood forest stands was evaluated using two soil carbon models, CENTURY and YASSO, that were calibrated with forest plot empirical data in the Green Mountains of Vermont. Overall, 13 different harvesting scenarios that included four levels of aboveground biomass removal (20%, 40%, 60% and 90%) and four different rotation lengths (60 year, 90 year, 120 year, and No Rotation (NR)) were simulated for a 360 year period. Simulations indicate that following an initial post-harvest increase, total SOC decreases for several decades until carbon inputs into the soil pool from the re-growth are greater than losses due to decomposition. At this point total SOC begins to gradually increase until the next harvest. One consequence of this recovery pattern is that between harvests, the size of the SOC pool in a stand may change from -7 to 18% of the pre-harvest pool, depending on the soil pool considered. Over 360 years, the average annual decrease in total SOC depends on the amount of biomass removed, the rotation length, and the soil pool considered. After 360 years a stand undergoing the 90yr-40% scenario will have 15% less total SOC than a non-harvested stand. Longterm declines in total SOC greater than 10% were observed in the 60yr-60%, 60yr-90%, and 90yr-90% scenarios. Long-term declines less than 5% were observed in scenarios with 120 year rotations that remove 60% or less of the aboveground biomass. The long-term decreases simulated here for common management scenarios in this region would require intensive sampling procedures to be detectable. (C) 2009 Elsevier B.V. All rights reserved. C1 [Johnson, Kristofer; Scatena, Frederick N.] Univ Penn, Dept Earth & Environm Sci, Philadelphia, PA 19104 USA. [Pan, Yude] US Forest Serv, No Res Stn, USDA, Newtown Sq, PA 19073 USA. RP Johnson, K (reprint author), Univ Penn, Dept Earth & Environm Sci, 240 S 33rd St, Philadelphia, PA 19104 USA. EM ffkdj@uaf.edu RI Pan, Yude/F-6145-2015 NR 47 TC 20 Z9 20 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1262 EP 1267 DI 10.1016/j.foreco.2009.06.049 PG 6 WC Forestry SC Forestry GA 572ES UT WOS:000275810900008 ER PT J AU Sun, G Noormets, A Gavazzi, MJ McNulty, SG Chen, J Domec, JC King, JS Amatya, DM Skaggs, RW AF Sun, G. Noormets, A. Gavazzi, M. J. McNulty, S. G. Chen, J. Domec, J. -C. King, J. S. Amatya, D. M. Skaggs, R. W. TI Energy and water balance of two contrasting loblolly pine plantations on the lower coastal plain of North Carolina, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article; Proceedings Paper CT Conference of the Landscape-Ecology-Working-Group of the International-Union-of-Forest-Research-Organizations CY SEP 16-22, 2008 CL Chengdu, PEOPLES R CHINA SP Int Union Forest Res Org, Landscape Ecol Working Grp, USDA Forest Serv DE Energy balance; Evapotranspiration; Forest albedo; Forest hydrology; Loblolly pine; Water balance ID SOUTHEASTERN UNITED-STATES; SONIC ANEMOMETER; EDDY COVARIANCE; BOREAL FOREST; CARBON; EVAPOTRANSPIRATION; HYDROLOGY; EXCHANGE; FLUXES; CHRONOSEQUENCE AB During 2005-2007, we used the eddy covariance and associated hydrometric methods to construct energy and water budgets along a chronosequence of loblolly pine (Pinus taeda) plantations that included a mid-rotation stand (LP) (i.e., 13-15 years old) and a recently established stand on a clearcut site (CC) (i.e., 4-6 years old) in Eastern North Carolina. Our central objective was to quantify the differences in both energy and water balances between the two contrasting stands and understand the underlining mechanisms of environmental controls. We found that the LP site received about 20% more net radiation (R(n)) due to its lower averaged albedo (alpha) of 0.25, compared with that at the CC (alpha = 0.34). The mean monthly averaged Bowen ratios (beta) at the LP site were 0.89 +/- 0.7, significantly (p = 0.02) lower than at the CC site (1.45 +/- 1.2). Higher net radiation resulted in a 28% higher (p = 0.02) latent heat flux (LE) for ecosystem evapotranspiration at the LP site, but there was no difference in sensible heat flux (H) between the two contrasting sites. The annual total evapotranspiration (ET) at the LP site and CC site was estimated as 1011-1226 and 755-855 mm year(-1), respectively. The differences in ET rates between the two contrasting sites occurred mostly during the non-growing seasons and/or dry periods, and they were small during peak growing seasons or wet periods. Higher net radiation and biomass in LP were believed to be responsible to the higher ET. The monthly ET/Grass Reference ET ratios differed significantly across site and season. The annual ET/P ratio for the LP and CC were estimated as 0.70-1.13 and 0,60-0.88, respectively, indicating higher runoff production from the CC site than the LP site. This study implied that reforestation practices reduced Surface albedos and thus increased available energy, but they did not necessarily increase energy for warming the atmosphere in the coastal plain region where soil water was generally not limited. This study showed the highly variable response of energy and water balances to forest management due to climatic variability. Published by Elsevier B.V. C1 [Sun, G.; Gavazzi, M. J.; McNulty, S. G.] US Forest Serv, So Global Change Program, So Res Stn, USDA, Raleigh, NC 27606 USA. [Noormets, A.; Domec, J. -C.; King, J. S.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Chen, J.] Univ Toledo, Dept Earth Ecol & Environm Sci, Toledo, OH 43606 USA. [Amatya, D. M.] US Forest Serv, Ctr Forested Wetlands Res, USDA, Cordesville, SC 29434 USA. [Skaggs, R. W.] N Carolina State Univ, Dept Biol & Agr Engn, Raleigh, NC 27695 USA. RP Sun, G (reprint author), US Forest Serv, So Global Change Program, So Res Stn, USDA, 920 Main Campus Dr,Suite 300,Venture 2, Raleigh, NC 27606 USA. EM Ge_Sun@ncsu.edu RI Chen, Jiquan/D-1955-2009; Noormets, Asko/A-7257-2009 OI Noormets, Asko/0000-0003-2221-2111 NR 68 TC 70 Z9 73 U1 4 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1299 EP 1310 DI 10.1016/j.foreco.2009.09.016 PG 12 WC Forestry SC Forestry GA 572ES UT WOS:000275810900012 ER PT J AU Tian, HQ Chen, GS Liu, ML Zhang, C Sun, G Lu, CQ Xu, XF Ren, W Pan, SF Chappelka, A AF Tian, Hanqin Chen, Guangsheng Liu, Mingliang Zhang, Chi Sun, Ge Lu, Chaoqun Xu, Xiaofeng Ren, Wei Pan, Shufen Chappelka, Arthur TI Model estimates of net primary productivity, evapotranspiration, and water use efficiency in the terrestrial ecosystems of the southern United States during 1895-2007 SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article; Proceedings Paper CT Conference of the Landscape-Ecology-Working-Group of the International-Union-of-Forest-Research-Organizations CY SEP 16-22, 2008 CL Chengdu, PEOPLES R CHINA SP Int Union Forest Res Org, Landscape Ecol Working Grp, USDA Forest Serv DE Net primary productivity (NPP); Evapotranspiration (ET); Dynamic Land Ecosystem Model (DLEM); Water use efficiency (WUE); Southern United States ID LAND-COVER DATABASE; RAIN-USE EFFICIENCY; CARBON SEQUESTRATION; STOMATAL CONDUCTANCE; CLIMATE VARIABILITY; PLANT-RESPONSES; FOREST; OZONE; PHOTOSYNTHESIS; VEGETATION AB The effects of global change on ecosystem productivity and water resources in the southern United States (SUS), a traditionally 'water-rich' region and the 'timber basket' of the country, are not well quantified. We carried out several simulation experiments to quantify ecosystem net primary productivity (NPP), evapotranspiration (ET) and water use efficiency (WUE) (i.e., NPP/ET) in the SUS by employing an integrated process-based ecosystem model (Dynamic Land Ecosystem Model, DLEM). The results indicated that the average ET in the SUS was 710 mm during 1895-2007. As a whole, the annual ET increased and decreased slightly during the first and second half of the study period, respectively. The mean regional total NPP was 1.18PgC/yr (525.2gC/m(2)/yr) during 1895-2007. NPP increased consistently from 1895 to 2007 with a rate of 2.5 Tg C/yr or 1.10 g C/m(2)/yr, representing a 27% increase. The average WUE was about 0.71 g C/kg H(2)O and increased about 25% from 1895 to 2007. The rather stable ET might explain the resulting increase in WUE. The average WUE of different biomes followed an order of: forest (0.93gC/kgH(2)O)>wetland (0.75 gC/kgH(2)O) > grassland (0.58gC/kg H(2)O) > cropland (0.54 g C/kg H(2)O) > shrubland (0.45 g C/kg H(2)O). WUE of cropland increased the fastest (by 30%). followed by shrubland (17%) and grassland (9%), while WUE of forest and wetland changed little from the period of 1895-1950 to the period of 1951-2007. NPP, ET and WUE showed substantial inter-annual and spatial variability, which was induced by the non-uniform distribution patterns and change rates of environmental factors across the SUS. We concluded that an accurate projection of the regional impact of climate change on carbon and water resources must consider the spatial variability of ecosystem water use efficiency across biomes as well as the interactions among all stresses, especially land-use and land-cover change and climate. (C) 2009 Elsevier B.V. All rights reserved. C1 [Tian, Hanqin; Chen, Guangsheng; Liu, Mingliang; Zhang, Chi; Lu, Chaoqun; Xu, Xiaofeng; Ren, Wei; Pan, Shufen; Chappelka, Arthur] Auburn Univ, Sch Forestry & Wildlife Sci, Ecosyst Dynam & Global Ecol Lab, Auburn, AL 36849 USA. [Sun, Ge] US Forest Serv, So Global Change Program, USDA, Raleigh, NC 27606 USA. RP Tian, HQ (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, Ecosyst Dynam & Global Ecol Lab, Auburn, AL 36849 USA. EM tianhan@auburn.edu RI Liu, Mingliang/B-1361-2009; Lu, Chaoqun/A-6497-2012; Tian, Hanqin/A-6484-2012; Xu, Xiaofeng/B-2391-2008; Ren, Wei/G-8317-2016; Ren, Wei/I-4048-2014 OI Tian, Hanqin/0000-0002-1806-4091; Xu, Xiaofeng/0000-0002-6553-6514; Ren, Wei/0000-0002-4840-4835 NR 78 TC 107 Z9 119 U1 14 U2 81 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 20 PY 2010 VL 259 IS 7 SI SI BP 1311 EP 1327 DI 10.1016/j.foreco.2009.10.009 PG 17 WC Forestry SC Forestry GA 572ES UT WOS:000275810900013 ER PT J AU Choi, MY Jurenka, RA AF Choi, Man-Yeon Jurenka, Russell A. TI Site-directed mutagenesis and PBAN activation of the Helicoverpa zea PBAN-receptor SO FEBS LETTERS LA English DT Article DE PBAN-receptor; GPCR; Point mutation; Extracellular domain; Pyrokinin ID PROTEIN-COUPLED RECEPTORS; CRYSTAL-STRUCTURE; SWISS-MODEL; EXTRACELLULAR DOMAINS; NEUROPEPTIDE; IDENTIFICATION; LEPIDOPTERA; EXPRESSION; NOCTUIDAE; BINDING AB Pheromone biosynthesis-activating neuropeptide ( PBAN) and pyrokinins belong to a family of insect peptide hormones that have a common FXPRLamide C-terminal ending. The G-protein-coupled receptors (GPCRs) for this peptide family were first identified from a moth and Drosophila with sequence similarity to neuromedin U receptors from vertebrates. We have characterized the PBAN-receptor (PBAN-R or PR) active binding domains using chimeric GPCRs and proposed that extracellular loop 3 is critical for ligand selection. Here, we characterized the 3rd extracellular domain of PBAN-R through site-directed point mutations. Results are discussed in context of the structural features required for receptor activation using receptor activation experiments and in silico computational modeling. This research will help in characterizing these receptors towards a goal of finding agonists and/or antagonists for PBAN/pyrokinin receptors. Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies. C1 [Choi, Man-Yeon] ARS, USDA, Gainesville, FL 32608 USA. [Choi, Man-Yeon; Jurenka, Russell A.] Iowa State Univ, Dept Entomol, Ames, IA USA. RP Choi, MY (reprint author), ARS, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM mychoi@ars.usda.gov; rjurenka@iastate.edu OI Choi, Man-Yeon/0000-0003-0769-380X FU US-Israel Binational Agricultural Research and Development Fund [IS-4163-08C]; State of Iowa funds FX We thank Steven Valles, Mike Scharf, and anonymous reviewers for valuable review and comments. This research was funded by research grant No. IS-4163-08C, the US-Israel Binational Agricultural Research and Development Fund and State of Iowa funds. NR 27 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD MAR 19 PY 2010 VL 584 IS 6 BP 1212 EP 1216 DI 10.1016/j.febslet.2010.02.033 PG 5 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 571GC UT WOS:000275738700023 PM 20159019 ER PT J AU Mullin, CA Frazier, M Frazier, JL Ashcraft, S Simonds, R vanEngelsdorp, D Pettis, JS AF Mullin, Christopher A. Frazier, Maryann Frazier, James L. Ashcraft, Sara Simonds, Roger vanEngelsdorp, Dennis Pettis, Jeffery S. TI High Levels of Miticides and Agrochemicals in North American Apiaries: Implications for Honey Bee Health SO PLOS ONE LA English DT Article ID APIS-MELLIFERA L; COLONY COLLAPSE DISORDER; PESTICIDE-RESIDUES; PYRETHROID INSECTICIDE; SYSTEMIC INSECTICIDES; ACARICIDE RESIDUES; RISK-ASSESSMENT; VARROA MITES; HYMENOPTERA; PRODUCTS AB Background: Recent declines in honey bees for crop pollination threaten fruit, nut, vegetable and seed production in the United States. A broad survey of pesticide residues was conducted on samples from migratory and other beekeepers across 23 states, one Canadian province and several agricultural cropping systems during the 2007-08 growing seasons. Methodology/Principal Findings: We have used LC/MS-MS and GC/MS to analyze bees and hive matrices for pesticide residues utilizing a modified QuEChERS method. We have found 121 different pesticides and metabolites within 887 wax, pollen, bee and associated hive samples. Almost 60% of the 259 wax and 350 pollen samples contained at least one systemic pesticide, and over 47% had both in-hive acaricides fluvalinate and coumaphos, and chlorothalonil, a widely-used fungicide. In bee pollen were found chlorothalonil at levels up to 99 ppm and the insecticides aldicarb, carbaryl, chlorpyrifos and imidacloprid, fungicides boscalid, captan and myclobutanil, and herbicide pendimethalin at 1 ppm levels. Almost all comb and foundation wax samples (98%) were contaminated with up to 204 and 94 ppm, respectively, of fluvalinate and coumaphos, and lower amounts of amitraz degradates and chlorothalonil, with an average of 6 pesticide detections per sample and a high of 39. There were fewer pesticides found in adults and brood except for those linked with bee kills by permethrin (20 ppm) and fipronil (3.1 ppm). Conclusions/Significance: The 98 pesticides and metabolites detected in mixtures up to 214 ppm in bee pollen alone represents a remarkably high level for toxicants in the brood and adult food of this primary pollinator. This represents over half of the maximum individual pesticide incidences ever reported for apiaries. While exposure to many of these neurotoxicants elicits acute and sublethal reductions in honey bee fitness, the effects of these materials in combinations and their direct association with CCD or declining bee health remains to be determined. C1 [Mullin, Christopher A.; Frazier, Maryann; Frazier, James L.; Ashcraft, Sara] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Simonds, Roger] Agr Mkt Serv, Natl Sci Lab, USDA, Gastonia, NC USA. [vanEngelsdorp, Dennis] Penn Dept Agr, Harrisburg, PA USA. [Pettis, Jeffery S.] ARS, Bee Res Lab, USDA, Beltsville, MD USA. RP Mullin, CA (reprint author), Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. EM camullin@psu.edu RI vanEngelsdorp, Dennis/E-7934-2010 FU Florida State Beekeepers; National Honey Board; Penn State College of Agriculture Sciences; Project Apis mellifera (PAm); Tampa Bay Beekeepers; The Foundation for the Preservation of Honey Bees; United States Department of Agriculture FX Funding was received from the Florida State Beekeepers, National Honey Board, Penn State College of Agriculture Sciences, Project Apis mellifera (PAm), Tampa Bay Beekeepers, The Foundation for the Preservation of Honey Bees, and the United States Department of Agriculture Critical Issues program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 77 TC 325 Z9 335 U1 55 U2 400 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 185 BERRY ST, STE 1300, SAN FRANCISCO, CA 94107 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 19 PY 2010 VL 5 IS 3 AR e9754 DI 10.1371/journal.pone.0009754 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 572EH UT WOS:000275809700007 PM 20333298 ER PT J AU Ellingson, JS Wang, Y Layton, S Ciacci-Zanella, J Roof, MB Faaberg, KS AF Ellingson, Joshua S. Wang, Yue Layton, Sarah Ciacci-Zanella, Janice Roof, Michael B. Faaberg, Kay S. TI Vaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras SO VACCINE LA English DT Article DE Porcine reproductive and respiratory syndrome virus (PRRSV); Infectious clones; Vaccine study; Ingelvac (R) PRRS MLV; Strain MN184; Chimeric viruses ID IMMUNE-RESPONSE; UNITED-STATES; NSP2; PRRSV; STRAIN; IDENTIFICATION; VIRULENCE; DELETION; REGION; CHINA AB The vaccine efficacy of six PRRSV Type 2 infectious clones, including five chimeras and a strain-specific deletion mutant, were examined using a respiratory challenge model in growing swine. The chimeras were constructed from different combinations of a licensed modified live vaccine (Ingelvac (R) PRRS MLV) and a virulent field isolate (wt MN184) which differ by 14.3% on a nucleotide basis, while the deletion mutant tested had a broad deletion in the nsp2 region of strain MN184. The appearance of antibodies and virus characterization revealed regions of the genome that could influence PRRSV replication in vivo. Swine growth, clinical signs and lung lesions were also monitored. Average daily weight gain was negatively and directly impacted by some vaccines, and after challenge, vaccination with different constructs led to variable weight gain. We determined that 3 of the tested chimeras, including two previously published chimeras [1] and one in which strain MN184 ORF5-6 was placed on the background of Ingelvac PRRS MLV were able to prevent lung consolidation to a similar extent as traditionally prepared cell-passaged attenuated vaccines. The study suggested that only specific chimeras can attenuate clinical signs in swine and that attenuation cannot be directly linked to primary virus replication. Additionally, the strain MN184 deletion mutant was not found to have been sufficiently attenuated nor efficacious against heterologous challenge with strain JA-142. Published by Elsevier Ltd. C1 [Ciacci-Zanella, Janice; Faaberg, Kay S.] USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Ellingson, Joshua S.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. [Wang, Yue; Faaberg, Kay S.] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. [Layton, Sarah; Roof, Michael B.] Boehringer Ingelheim Vetmed Inc, Ames, IA 50010 USA. RP Faaberg, KS (reprint author), USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave,POB 70,Mail Stop 2S-209, Ames, IA 50010 USA. EM kay.faaberg@ars.usda.gov RI Zanella, Janice/C-3632-2014 NR 34 TC 21 Z9 23 U1 0 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 19 PY 2010 VL 28 IS 14 BP 2679 EP 2686 DI 10.1016/j.vaccine.2009.12.073 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 581FA UT WOS:000276505700015 PM 20064475 ER PT J AU Ma, LJ van der Does, HC Borkovich, KA Coleman, JJ Daboussi, MJ Di Pietro, A Dufresne, M Freitag, M Grabherr, M Henrissat, B Houterman, PM Kang, S Shim, WB Woloshuk, C Xie, XH Xu, JR Antoniw, J Baker, SE Bluhm, BH Breakspear, A Brown, DW Butchko, RAE Chapman, S Coulson, R Coutinho, PM Danchin, EGJ Diener, A Gale, LR Gardiner, DM Goff, S Hammond-Kosack, KE Hilburn, K Hua-Van, A Jonkers, W Kazan, K Kodira, CD Koehrsen, M Kumar, L Lee, YH Li, LD Manners, JM Miranda-Saavedra, D Mukherjee, M Park, G Park, J Park, SY Proctor, RH Regev, A Ruiz-Roldan, MC Sain, D Sakthikumar, S Sykes, S Schwartz, DC Turgeon, BG Wapinski, I Yoder, O Young, S Zeng, QD Zhou, SG Galagan, J Cuomo, CA Kistler, HC Rep, M AF Ma, Li-Jun van der Does, H. Charlotte Borkovich, Katherine A. Coleman, Jeffrey J. Daboussi, Marie-Josee Di Pietro, Antonio Dufresne, Marie Freitag, Michael Grabherr, Manfred Henrissat, Bernard Houterman, Petra M. Kang, Seogchan Shim, Won-Bo Woloshuk, Charles Xie, Xiaohui Xu, Jin-Rong Antoniw, John Baker, Scott E. Bluhm, Burton H. Breakspear, Andrew Brown, Daren W. Butchko, Robert A. E. Chapman, Sinead Coulson, Richard Coutinho, Pedro M. Danchin, Etienne G. J. Diener, Andrew Gale, Liane R. Gardiner, Donald M. Goff, Stephen Hammond-Kosack, Kim E. Hilburn, Karen Hua-Van, Aurelie Jonkers, Wilfried Kazan, Kemal Kodira, Chinnappa D. Koehrsen, Michael Kumar, Lokesh Lee, Yong-Hwan Li, Liande Manners, John M. Miranda-Saavedra, Diego Mukherjee, Mala Park, Gyungsoon Park, Jongsun Park, Sook-Young Proctor, Robert H. Regev, Aviv Carmen Ruiz-Roldan, M. Sain, Divya Sakthikumar, Sharadha Sykes, Sean Schwartz, David C. Turgeon, B. Gillian Wapinski, Ilan Yoder, Olen Young, Sarah Zeng, Qiandong Zhou, Shiguo Galagan, James Cuomo, Christina A. Kistler, H. Corby Rep, Martijn TI Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium SO NATURE LA English DT Article ID MAXIMUM-LIKELIHOOD; OXYSPORUM; GENE; SEQUENCE; TOMATO; EXPRESSION; RESISTANCE; POLYMORPHISM; ANNOTATION; INFECTION AB Fusarium species are among the most important phytopathogenic and toxigenic fungi. To understand the molecular underpinnings of pathogenicity in the genus Fusarium, we compared the genomes of three phenotypically diverse species: Fusarium graminearum, Fusarium verticillioides and Fusarium oxysporum f. sp. lycopersici. Our analysis revealed lineage-specific (LS) genomic regions in F. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. LS regions are rich in transposons and genes with distinct evolutionary profiles but related to pathogenicity, indicative of horizontal acquisition. Experimentally, we demonstrate the transfer of two LS chromosomes between strains of F. oxysporum, converting a non-pathogenic strain into a pathogen. Transfer of LS chromosomes between otherwise genetically isolated strains explains the polyphyletic origin of host specificity and the emergence of new pathogenic lineages in F. oxysporum. These findings put the evolution of fungal pathogenicity into a new perspective. C1 [Breakspear, Andrew; Gale, Liane R.; Hilburn, Karen; Kistler, H. Corby] Univ Minnesota, USDA ARS, St Paul, MN 55108 USA. [Ma, Li-Jun; Grabherr, Manfred; Chapman, Sinead; Kodira, Chinnappa D.; Koehrsen, Michael; Kumar, Lokesh; Regev, Aviv; Sakthikumar, Sharadha; Sykes, Sean; Wapinski, Ilan; Young, Sarah; Zeng, Qiandong; Galagan, James; Cuomo, Christina A.] Broad Inst, Cambridge, MA 02141 USA. [van der Does, H. Charlotte; Houterman, Petra M.; Jonkers, Wilfried; Rep, Martijn] Univ Amsterdam, NL-1098 XH Amsterdam, Netherlands. [Borkovich, Katherine A.; Li, Liande; Park, Gyungsoon; Sain, Divya] Univ Calif Riverside, Riverside, CA 92521 USA. [Coleman, Jeffrey J.] Univ Arizona, Tucson, AZ 85721 USA. [Daboussi, Marie-Josee; Hua-Van, Aurelie] Univ Paris 11, F-91405 Paris, France. [Di Pietro, Antonio; Carmen Ruiz-Roldan, M.] Univ Cordoba, E-14071 Cordoba, Spain. [Freitag, Michael] Oregon State Univ, Corvallis, OR 97331 USA. [Henrissat, Bernard; Coutinho, Pedro M.; Danchin, Etienne G. J.] Univ Aix Marseille, CNRS, F-13628 Aix En Provence, France. [Kang, Seogchan; Park, Sook-Young] Penn State Univ, University Pk, PA 16802 USA. [Shim, Won-Bo; Mukherjee, Mala] Texas A&M Univ, College Stn, TX 77843 USA. [Woloshuk, Charles; Xu, Jin-Rong; Bluhm, Burton H.] Purdue Univ, W Lafayette, IN 47907 USA. [Xie, Xiaohui] Univ Calif Irvine, Irvine, CA 92697 USA. [Antoniw, John; Hammond-Kosack, Kim E.] Ctr Sustainable Pest & Dis Management, Harpenden AL5 2JQ, Herts, England. [Baker, Scott E.] Pacific NW Natl Lab, Richland, WA 99352 USA. [Brown, Daren W.; Butchko, Robert A. E.; Proctor, Robert H.] USDA ARS NCAUR, Peoria, IL 61604 USA. [Coulson, Richard] European Bioinformat Inst, Cambridge CB10 1SD, England. [Diener, Andrew] Univ Calif Los Angeles, Los Angeles, CA 90095 USA. [Gardiner, Donald M.; Kazan, Kemal; Manners, John M.] CSIRO Plant Ind, Brisbane, Qld 4067, Australia. [Goff, Stephen] Univ Arizona, Inst BIO5, Tucson, AZ 85721 USA. [Lee, Yong-Hwan; Park, Jongsun] Seoul Natl Univ, Seoul 151742, South Korea. [Miranda-Saavedra, Diego] Cambridge Inst Med Res, Cambridge CB2 0XY, England. [Schwartz, David C.; Zhou, Shiguo] Univ Wisconsin, Madison, WI 53706 USA. [Turgeon, B. Gillian] Cornell Univ, Ithaca, NY 14853 USA. RP Kistler, HC (reprint author), Univ Minnesota, USDA ARS, St Paul, MN 55108 USA. EM hckist@umn.edu; m.rep@uva.nl RI Gardiner, Donald/A-1297-2010; li, liande/C-9985-2011; Biology, Transformational/D-5787-2011; Miranda-Saavedra, Diego/C-2707-2012; Manners, John/A-4922-2008; Kazan, Kemal/B-8032-2008; Henrissat, Bernard/J-2475-2012; Danchin, Etienne/A-6648-2008; Zhou, Shiguo/B-3832-2011; Hua-Van, Aurelie/N-3495-2013; Coleman, Jeffrey/E-2981-2015; Di Pietro, Antonio/K-9220-2014 OI Galagan, James/0000-0003-0542-3291; Coleman, Jeffrey/0000-0001-8579-1996; Ruiz Roldan, M. Carmen/0000-0003-3427-2998; Broz, Karen/0000-0001-7928-7216; Park, Jongsun/0000-0003-0786-4701; Cuomo, Christina/0000-0002-5778-960X; Ma, Li-Jun/0000-0002-2733-3708; Gardiner, Donald/0000-0002-2162-8716; Danchin, Etienne/0000-0003-4146-5608; Zhou, Shiguo/0000-0001-7421-2506; Di Pietro, Antonio/0000-0001-5930-5763 FU USDA's National Institute of Food and Agriculture [2005-35600-16405] FX The 4 X sequence of F. verticillioides was provided by Syngenta Biotechnology Inc. Generation of the other 4 X sequence of F. verticillioides and 6.83 sequence of F. oxysporum f. sp. lycopersici was funded by the National Research Initiative of USDA's National Institute of Food and Agriculture through the Microbial Genome Sequencing Program (2005-35600-16405) and conducted by the Broad Institute Sequencing Platform. Wayne Xu and the Minnesota Supercomputing Institute for Advanced Computational Research are also acknowledged for their support. The authors thank Leslie Gaffney at the Broad Institute for graphic design and editing and Tracy E. Anderson of the University of Minnesota, College of Biological Sciences Imaging Center for spore micrographs. NR 37 TC 485 Z9 744 U1 23 U2 179 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 J9 NATURE JI Nature PD MAR 18 PY 2010 VL 464 IS 7287 BP 367 EP 373 DI 10.1038/nature08850 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 570FG UT WOS:000275657100037 PM 20237561 ER PT J AU Lahiji, RR Xu, X Reifenberger, R Raman, A Rudie, A Moon, RJ AF Lahiji, Roya R. Xu, Xin Reifenberger, Ronald Raman, Arvind Rudie, Alan Moon, Robert J. TI Atomic Force Microscopy Characterization of Cellulose Nanocrystals SO LANGMUIR LA English DT Article ID ELASTIC-MODULUS; MICROCRYSTALLINE CELLULOSE; POLYMER NANOCOMPOSITES; NATIVE CELLULOSE; CRYSTALLINE REGIONS; BACTERIAL CELLULOSE; CANTILEVER DYNAMICS; I-ALPHA; WHISKERS; FIBERS AB Cellulose nanocrystals (CNCs) are gaining interest as a "green" nanomaterial with superior mechanical and chemical properties for high-performance nanocomposite materials; however, there is a lack of accurate material property characterization of individual CNCs. Here, a detailed Study of the topography, elastic and adhesive properties of individual wood-derived CNCs is performed using atomic force microscopy (AFM). AFM experiments involving high-resolution dynamic mode imaging and jump-mode measurements were performed on individual CNCs under ambient conditions with 30% relative humidity (RH) and under a N(2) atmosphere with 0.1% RH. A procedure was also developed to calculate the CNC transverse elastic modulus (E(T)) by comparing the experimental force-distance curves measured on the CNCs with 3D finite element calculations of tip indentation on the CNC. The E(T) of ail isolated CNC was estimated to be between 18 and 50 GPa at 0.1% RH; however, the associated crystallographic orientation of the CNC could not be determined. CNC properties were reasonably uniform along the entire CNC length, despite variations along the axis of 3-8 rim in CNC height. The range of RH used in this study was found to have a minimal effect oil the CNC geometry, confirming the resistance of the cellulose crystals to water penetration. CNC flexibility was also investigated by using the AFM tip as a nanomanipulator. C1 [Lahiji, Roya R.; Xu, Xin; Reifenberger, Ronald; Raman, Arvind; Moon, Robert J.] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA. [Lahiji, Roya R.; Reifenberger, Ronald] Purdue Univ, Dept Phys, W Lafayette, IN 47907 USA. [Xu, Xin; Raman, Arvind] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Rudie, Alan] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Lahiji, RR (reprint author), Purdue Univ, Birck Nanotechnol Ctr, 1205 W State St, W Lafayette, IN 47907 USA. EM rlahiji@purdue.edu; robertmoon@fs.fed.us RI Xu, Xin/B-1975-2014 OI Xu, Xin/0000-0002-4876-5710 FU US Forest Services and Discovery Park at Purdue University FX We thank the US Forest Services and Discovery Park at Purdue University for funding this project. We are grateful to Pablo Ares from Nanotec Electronica for his Continuous assistance with the WSxM software, Rick Reiner of the US Forest Service for processing the cellulose nanocrystals, and Jim Beecher of the US Forest Service for many useful discussions. NR 62 TC 92 Z9 94 U1 5 U2 93 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0743-7463 J9 LANGMUIR JI Langmuir PD MAR 16 PY 2010 VL 26 IS 6 BP 4480 EP 4488 DI 10.1021/la903111j PG 9 WC Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary SC Chemistry; Materials Science GA 564PE UT WOS:000275226700103 PM 20055370 ER PT J AU Ingebritson, AL Roth, JA Hauer, PJ AF Ingebritson, Alaina L. Roth, James A. Hauer, Paull J. TI Erysipelothrix rhusiopathiae: Association of Spa-type with serotype and role in protective immunity SO VACCINE LA English DT Article DE Erysipelothrix rhusiopathiae; Surface protective antigen; Erysipelas ID CROSS-PROTECTION; ANTIGEN; SWINE; STRAINS; MICE; TONSILLARUM; CHALLENGE; SEROVARS; VACCINE; PIGS AB A collection of swine, fish, and cetacean Erysipelothrix rhusiopathiae strains representing 16 serotypes was analyzed for possession of the three currently recognized surface protective antigen (spa)-types: spaA, spaB, and spaC. Polymerase chain reaction (PCR) assays and Western blotting with a SpaA-specific monoclonal antibody demonstrated that spa-type is not confined to specific serotype groups. In particular, the spa-type of strains of aquatic origin was more variable than those of terrestrial origin, and possessed the distinct ability to express more than one spa. In a cross-protection study, mice immunized with an E. rhusiopathiae serotype 2 SpaA-type strain and challenged with various E. rhusiopathiae isolates were completely protected against strains exhibiting a single homologous spa, but variably protected against strains possessing a heterologous spa or those harboring more than one spa-type. Published by Elsevier Ltd. C1 [Ingebritson, Alaina L.] Anim & Plant Hlth Inspect Serv, Ctr Vet Biol, Vet Serv, USDA, Ames, IA 50010 USA. [Roth, James A.] Iowa State Univ, Coll Vet Med, Ames, IA 50010 USA. [Hauer, Paull J.] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, Vet Serv, USDA, Ames, IA 50010 USA. RP Ingebritson, AL (reprint author), Anim & Plant Hlth Inspect Serv, Ctr Vet Biol, Vet Serv, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM Alaina.LIngebritson@aphis.usda.gov RI Roth, James/A-7122-2009 OI Roth, James/0000-0003-3562-668X FU USDA/APHIS/VS-National Veterinary Services Laboratories; Center for Veterinary Biologics Graduate Training program FX This work was supported by the USDA/APHIS/VS-National Veterinary Services Laboratories and the Center for Veterinary Biologics Graduate Training program. NR 26 TC 12 Z9 13 U1 1 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 16 PY 2010 VL 28 IS 13 BP 2490 EP 2496 DI 10.1016/j.vaccine.2010.01.041 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 578JV UT WOS:000276290500003 PM 20117264 ER PT J AU Runyon, JB Robinson, H AF Runyon, Justin B. Robinson, Harold TI Hurleyella, a new genus of Nearctic Dolichopodidae (Diptera) SO ZOOTAXA LA English DT Article DE long-legged flies; Medeterinae; micro-dolichopodid; Enlinia; Harmstonia; Microcyrtura; Microchrysotus; Micromedetera ID REVISION AB The new micro-dolichopodid genus Hurleyella and two new species, H. cumberlandensis and H. brooksi are described from the Nearctic. Hurleyella resembles the subfamily Medeterinae in having the legs bare of major setae, a depressed posterior mesonotum, and concave dorsal postcranium, but the complex male genitalia is unlike the relatively simple structure of traditional Medeterinae (e. g., Medetera, Thrypyicus, Corindia, etc.). Until the relationship of Hurleyella to other genera can be determined, and the limits of dolichopodid subfamilies refined, it should be considered as incertae sedis. C1 [Runyon, Justin B.] US Forest Serv, Rocky Mt Res Stn, USDA, Bozeman, MT 59717 USA. [Runyon, Justin B.] Montana State Univ, Bozeman, MT 59717 USA. [Robinson, Harold] Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20013 USA. RP Runyon, JB (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, 1648 S 7th Ave, Bozeman, MT 59717 USA. EM jrunyon@montana.edu OI Runyon, Justin B./0000-0002-0271-0511 NR 20 TC 2 Z9 2 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 16 PY 2010 IS 2400 BP 57 EP 65 PG 9 WC Zoology SC Zoology GA 569MC UT WOS:000275600200006 ER PT J AU Fleisher, DH Timlin, DJ Yang, Y Reddy, VR AF Fleisher, David H. Timlin, Dennis J. Yang, Y. Reddy, V. R. TI Simulation of potato gas exchange rates using SPUDSIM SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Crop models; Potato; Gas exchange; Photosynthesis; Simulations; Carbon partitioning ID CONTROLLED-ENVIRONMENT CHAMBERS; INTERCEPTED SOLAR-RADIATION; CANOPY PHOTOSYNTHESIS; STOMATAL CONDUCTANCE; GROWTH CHAMBERS; ELEVATED CO2; CROP GROWTH; WATER-USE; LEAF; LEAVES AB SPUDSIM was developed from the model SIMPOTATO to incorporate mechanistic approaches for simulating photosynthesis and canopy growth and development needed to improve modeling accuracy for studies involving nutrient/water stress and climate change. Modifications included routines for simulating individual leaf appearance rates and leaf expansion as a function of leaf physiological age and plant assimilate status. Coupled sub-models for leaf-level photosynthesis, transpiration, and stomatal conductance were used to replace the older radiation use efficiency approach. A radiation transfer routine that estimated diffuse and direct-beam photosynthetically active radiation for sunlit and shaded leaves was also added. During each time increment, net photosynthetic rate was estimated for sunlit and shaded leaf area. Photosynthate was partitioned among leaves in the canopy according to leaf age, potential expansion, and plant assimilate status. Assimilate allocation to branches, roots, and tubers proceeded according to partitioning coefficients defined in the original model, SIMPOTATO. Remaining photosynthate was stored in the canopy and, when accumulated over a threshold amount, reduced leaf-level photosynthetic rate via feedback inhibition. Whole plant gas exchange and harvest data from SPAR (soil-plant-atmosphere research) chamber experiments conducted at USDA-ARS, Beltsville, MD were used to evaluate SPUDSIM predictions over a broad range of temperatures from 12.6 to 32.3 degrees C (24-h average basis). An additional independent SPAR chamber dataset was used to parameterize SPUDSIM crop coefficients. Root mean square error (RMSE) was less than 0.29 mol CO(2) m(-2) season(-1) for seasonal daily net assimilation rates and indices of agreement (IA) were 0.80 and higher except at the 32.3 degrees C study (0.62). Comparison of canopy photosynthetic rates at four different days indicated the model slightly under-predicted leaf area early in the season and over-predicted later in the season. IA and RMSE for leaf-level photosynthetic rates were above 0.88 and less than 1.6 mu mol CO(2) m(-2) s(-1) respectively for all studies except the 32.3 degrees C (0.61 and 3.8 mu mol CO(2) m(-2) s(-1)). Dry matter predictions fell within two standard deviations of measured values for most plant organs at harvest. Overall, these results indicated that SPUDSIM accurately captured potato growth and development responses over a wide-range of temperatures and will be suitable for a variety of applications involving complex soil-plant-atmosphere relationships. Published by Elsevier B.V. C1 [Fleisher, David H.; Timlin, Dennis J.; Reddy, V. R.] ARS, USDA, PSI, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Yang, Y.] Dow AgroSci LLC, Indianapolis, IN 46268 USA. RP Fleisher, DH (reprint author), ARS, USDA, PSI, Crop Syst & Global Change Lab, Bldg 001,Rm 342,BARC W,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM david.fleisher@ars.usda.gov NR 51 TC 10 Z9 10 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2010 VL 150 IS 3 BP 432 EP 442 DI 10.1016/j.agrformet.2010.01.005 PG 11 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 576CV UT WOS:000276122500012 ER PT J AU Aronson, EL McNulty, SG AF Aronson, Emma L. McNulty, Steven G. TI Reply to comment on "Appropriate experimental ecosystem warming methods by ecosystem, objective, and practicality" by Aronson and McNulty SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Editorial Material C1 [Aronson, Emma L.] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. [McNulty, Steven G.] US Forest Serv, USDA, So Res Stn, Raleigh, NC 27606 USA. RP Aronson, EL (reprint author), Univ Penn, Dept Biol, 433 S Univ Ave, Philadelphia, PA 19104 USA. EM emmala@sas.upenn.edu; smcnulty@fs.fed.us RI Aronson, Emma/I-3277-2012 NR 3 TC 4 Z9 4 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2010 VL 150 IS 3 BP 499 EP 500 DI 10.1016/j.agrformet.2009.11.021 PG 2 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 576CV UT WOS:000276122500020 ER PT J AU Lin, J Lee, IM Song, YQ Cook, NR Selhub, J Manson, JE Buring, JE Zhang, SM AF Lin, Jennifer Lee, I-Min Song, Yiqing Cook, Nancy R. Selhub, Jacob Manson, JoAnn E. Buring, Julie E. Zhang, Shumin M. TI Plasma Homocysteine and Cysteine and Risk of Breast Cancer in Women SO CANCER RESEARCH LA English DT Article ID FOOD FREQUENCY QUESTIONNAIRE; RANDOMIZED CONTROLLED-TRIAL; LOW-DOSE ASPIRIN; CARDIOVASCULAR-DISEASE; PRIMARY PREVENTION; METABOLIC SYNDROME; TUMOR-MARKER; FOLATE; HYPERHOMOCYSTEINEMIA; OBESITY AB Homocysteine and cysteine are associated with oxidative damage and metabolic disorders, which may lead to carcinogenesis. Observational studies assessing the association between circulating homocysteine or cysteine and breast cancer are very limited, and findings have been inconsistent. We prospectively evaluated plasma levels of homocysteine and cysteine in relation to breast cancer risk among 812 incident cases of invasive breast cancer and 812 individually matched control subjects from 28,345 women in the Women's Health Study; these women were >= 45 years old, provided blood samples, and had no history of cancer and cardiovascular disease at baseline. Logistic regression controlling for matching factors and risk factors for breast cancer was used to estimate relative risks (RR) and 95% confidence intervals (95% CI). All statistical tests were two sided. Homocysteine levels were not associated with overall risk for breast cancer. However, we observed a positive association between cysteine levels and breast cancer risk; the multivariate RR for the highest quintile group relative to the lowest quintile was 1.65 (95% CI, 1.04-2.61; P for trend = 0.04). In addition, women with higher levels of homocysteine and cysteine were at a greater risk for developing breast cancer when their folate levels were low (P for interaction = 0.04 and 0.002, respectively). Although our study offers little support for an association between circulating homocysteine and overall breast cancer risk, higher homocysteine levels may be associated with an increased risk for breast cancer among women with low folate status. The increased risk of breast cancer associated with high cysteine levels warrants further investigation. Cancer Res; 70(6); 2397-405. (C)2010 AACR. C1 [Lin, Jennifer; Lee, I-Min; Song, Yiqing; Cook, Nancy R.; Manson, JoAnn E.; Buring, Julie E.; Zhang, Shumin M.] Harvard Univ, Sch Med, Div Prevent Med, Brigham & Womens Hosp, Boston, MA 02215 USA. [Lee, I-Min; Cook, Nancy R.; Manson, JoAnn E.; Buring, Julie E.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Cambridge, MA 02138 USA. [Selhub, Jacob] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Medford, MA 02155 USA. [Buring, Julie E.] Brigham & Womens Hosp, Dept Ambulatory Care & Prevent, Boston, MA 02115 USA. RP Lin, J (reprint author), Harvard Univ, Sch Med, Div Prevent Med, Brigham & Womens Hosp, 900 Commonwealth Ave E, Boston, MA 02215 USA. EM jhlin@rics.bwh.harvard.edu FU National Cancer Institute [CA104871, CA47988, CA126846]; National Heart, Lung, and Blood Institute [HL043851, HL080467] FX Grant Support; National Cancer Institute grants CA104871, CA47988, and CA126846, and National Heart, Lung, and Blood Institute grants HL043851 and HL080467. NR 51 TC 33 Z9 34 U1 3 U2 8 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 2010 VL 70 IS 6 BP 2397 EP 2405 DI 10.1158/0008-5472.CAN-09-3648 PG 9 WC Oncology SC Oncology GA 607GA UT WOS:000278485900027 PM 20197471 ER PT J AU Vasquez-Mendez, R Ventura-Ramos, E Oleschko, K Hernandez-Sandoval, L Parrot, JF Nearing, MA AF Vasquez-Mendez, Rebeca Ventura-Ramos, Eusebio Oleschko, Klaudia Hernandez-Sandoval, Luis Parrot, Jean-Francois Nearing, Mark A. TI Soil erosion and runoff in different vegetation patches from semiarid Central Mexico SO CATENA LA English DT Article DE Dry lands; Runoff; Soil erosion; Vegetation patches ID RAINFALL EROSIVITY; PATAGONIAN STEPPE; RIVER-BASIN; HILLY AREA; CLIMATE; FACILITATION; COMPETITION; ECOSYSTEMS; CHINA AB Vegetation patches in and and semiarid areas are important in the regulation of surface hydrological processes. Canopy and ground covers developed in these fertility islands are a natural cushion against the impact energy of rainfall. Also, greater levels of organic matter improve the soil physicochemical properties, promoting infiltration and reducing runoff and soil erosion in comparison with the open spaces between them. During the 2006 rainy season. four USLE-type plots were installed around representative vegetation patches with predominant individual species of Huisache (Acacia sp), Mesquite (Prosopis sp), Prickly Pear or Nopal (Opuntia sp) and Cardon (Opuntia imbricata), to evaluate soil erosion and runoff, in semiarid Central Mexico. A comparative bare surface condition (Control) was also evaluated. Vegetative canopy and ground cover were computed using digital images. Selected soil parameters were determined. Soil erosion was different for the studied vegetation conditions, decreasing as canopy and ground cover increased. There were not significant differences in runoff and soil erosion between the Control and O. imbricata surfaces. Runoff was reduced by 87%, 87% and 98% and soil loss by 97%, 93%, and 99% for Acacia farnesiana, Prosopis laevigata and Opuntia sp, respectively, as compared to the Control. Soil surface physical conditions were different between the low vegetation cover conditions (Control and O. imbricata surfaces) and the greater vegetation cover conditions (A farnesiana, P. laevigata and Opuntia sp), indicating a positive effect of vegetation patches on the regulation of surface hydrological processes. (C) 2009 Elsevier B.V. All rights reserved. C1 [Vasquez-Mendez, Rebeca; Ventura-Ramos, Eusebio; Hernandez-Sandoval, Luis] Univ Queretaro, Sch Engn, Grad Program Environm & Water Resources Engn, CU Cerro Campanas, Queretaro 76010, Mexico. [Oleschko, Klaudia] Univ Nacl Autonoma Mexico, Ctr Geociencias, Queretaro 76001, Mexico. [Parrot, Jean-Francois] Univ Nacl Autonoma Mexico, Inst Geog, Mexico City 04510, DF, Mexico. [Nearing, Mark A.] ARS, USDA, SW Watershed Res Ctr, Tucson, AZ 85719 USA. RP Vasquez-Mendez, R (reprint author), Univ Queretaro, Sch Engn, Grad Program Environm & Water Resources Engn, CU Cerro Campanas, Queretaro 76010, Mexico. EM rebecavm@gmail.com FU SEMARNAT-CONACYT Environmental [SEMARNAT-2004-C01-240] FX This research was financially supported by the SEMARNAT-CONACYT Environmental Fund under the project SEMARNAT-2004-C01-240. NR 45 TC 47 Z9 53 U1 6 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 J9 CATENA JI Catena PD MAR 15 PY 2010 VL 80 IS 3 BP 162 EP 169 DI 10.1016/j.catena.2009.11.003 PG 8 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA 561QR UT WOS:000274994000003 ER PT J AU Stout, JE AF Stout, John E. TI Diurnal patterns of blowing sand SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE blowing sand; diurnal; saltation; aeolian; threshold AB The diurnal pattern of blowing sand results from a complex process that involves an interaction between solar heating, thermal instability, atmospheric turbulence, wind strength, and surface threshold conditions. During the day, solar heating produces thermal instability, which enhances the convective mixing of high momentum winds from the upper levels of the atmosphere to the surface layer. The sun also dries the sand surface so that the critical threshold is as low as possible. Thus, in the afternoon, the combination of strong turbulent winds and a low surface threshold increases the likelihood that winds may intermittently exceed the critical threshold of the surface to produce bursts of blowing sand. Here an attempt has been made to explore this dynamic aeolian process using a new method for monitoring the diurnal pattern of blowing sand. This technique involves detecting blowing sand with a piezoelectric saltation sensor to determine the relative proportion of time that blowing sand is detected for a given 'time of day'. Measurements taken over a seven-month period on the high plains of the Llano Estacado of West Texas and eastern New Mexico suggest that sand movement tends to occur more frequently during daylight hours with a peak in aeolian activity occurring in the afternoon between 14:00 and 15:00 Local Standard Time (LST). Published in 2010 by John Wiley & Sons, Ltd. C1 USDA ARS, Lubbock, TX 79415 USA. RP Stout, JE (reprint author), USDA ARS, 3810 4th St, Lubbock, TX 79415 USA. EM john.stout@ars.usda.gov NR 14 TC 18 Z9 19 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD MAR 15 PY 2010 VL 35 IS 3 BP 314 EP 318 DI 10.1002/esp.1919 PG 5 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 583LL UT WOS:000276677000008 ER PT J AU Chen, CYO Ribaya-Mercado, JD McKay, DL Croom, E Blumberg, JB AF Chen, C. -Y. Oliver Ribaya-Mercado, Judy D. McKay, Diane L. Croom, Edward Blumberg, Jeffrey B. TI Differential antioxidant and quinone reductase inducing activity of American, Asian, and Siberian ginseng SO FOOD CHEMISTRY LA English DT Article DE Ginseng; Antioxidant; Radicals; Quinone reductase; Extraction; Phenol ID RADICAL SCAVENGING ACTIVITY; RED GINSENG; PANAX-QUINQUEFOLIUS; CLINICAL-TRIALS; UNITED-STATES; IN-VITRO; EXTRACT; CONSTITUENTS; PREVENTION; INCREASE AB The antioxidant and quinone reductase (QR) inducing activities of American, Asian, and Siberian ginseng have been reported using various plant materials, solvents, and assays. To directly establish their comparative bioactivity, the effects of extracts obtained from acidified methanol (MeOH), a gastrointestinal mimic (GI), and hexane (Hex) on free radical scavenging and QR induction were tested. Siberian ginseng-MeOH had the highest total phenolic content at 52.6 mu mol gallic acid equivalents/g. GI liberated >= 50% more phenolics than MeOH and Hex from American and Asian ginseng. Siberian ginseng-Hex was most effective at inducing QR activity in Hepa1c1c7 cells. GI and MeOH extracts of American and Asian ginseng exhibited comparable HOCl scavenging activity, but were >= 4.6-fold more potent than Siberian ginseng. Siberian ginseng was the most effective scavenger of ONOO(-). Siberian-MeOH had the highest ferric reducing antioxidant power (FRAP), and American ginseng-GI had the highest oxygen radical absorbance capacity (ORAC). Thus, the components in ginseng have antioxidant and QR-inducing activity which are dependent upon the dose, method of extraction, radical species, and plant species. (C) 2009 Published by Elsevier Ltd. C1 [Chen, C. -Y. Oliver; Ribaya-Mercado, Judy D.; McKay, Diane L.; Blumberg, Jeffrey B.] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Croom, Edward] Croomia, Oxford, MS 38655 USA. RP Chen, CYO (reprint author), Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM oliver.chen@tufts.edu FU National Institutes of Health (NIH)/National Center on Complernentary and Alternative Medicine (NCCAM) [59-1950-7-735]; US Department of Agriculture (USDA)/Agricultural Research Service (ARS) [58-1950-7-707] FX We thank Jennifer O'Leary, Kendra Hamel, and Desiree Kelley for their excellent technical assistance. This study is supported by Interagency Agreement No. 59-1950-7-735 between the National Institutes of Health (NIH)/National Center on Complernentary and Alternative Medicine (NCCAM) and US Department of Agriculture (USDA)/Agricultural Research Service (ARS); and USDA ARS under Cooperative Agreement No. 58-1950-7-707. NR 34 TC 18 Z9 19 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2010 VL 119 IS 2 BP 445 EP 451 DI 10.1016/j.foodchem.2009.06.049 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 522BE UT WOS:000271971900003 ER PT J AU Lee, J Schreiner, RP AF Lee, Jungmin Schreiner, R. Paul TI Free amino acid profiles from 'Pinot noir' grapes are influenced by vine N-status and sample preparation method SO FOOD CHEMISTRY LA English DT Article DE Vitis vinifera; Pinot noir; Nutrient; Grape quality; YAN; Free amino acids; FAN; N; P; K ID VIRUSES GLRAV-2 AND-3; NITROGEN SUPPLEMENTATION; SACCHAROMYCES-CEREVISIAE; FRUIT COMPOSITION; FERMENTATION; JUICE; WINE; CULTIVARS; VOLATILE; PERFORMANCE AB This study examined the impact of extraction method on ammonia, free amino acids, and yeast assimilable nitrogen (YAN) concentrations in 'Pinot noir' berries obtained from a vine nutrition Study (altered supply of N, P, or K). Berries were either juiced or exhaustively extracted as whole berries prior to analysis. Extracts, compared to juice samples, had a significantly higher level of ammonia-N, assimilable amino acid-N, and YAN. For example, juice YAN values were approximately 50% of extract YAN values, when both were expressed in the same units. Free amino acid profiles and relative concentrations of individual amino acids were different in juice versus extracts, depending on how well the skin fraction was extracted prior to analysis. Lowering N Supply reduced free amino acids, with arginine being reduced more than the other 20 free amino acids identified in 'Pinot noir' berries. This was true in both juice and extracts. Since berry skin contributed to actual YAN, wineries that determine YAN from mainly the Pulp fraction (juice) May underestimate YAN and as a result add more (artificial) N supplement than is required for the healthy fermentation of red winemaking (whole berry fermentations). Extraction procedure should be taken into consideration when comparing grape YAN. (C) Published by Elsevier Ltd. C1 [Lee, Jungmin] ARS, USDA, PWA, Hort Crops Res Unit Worksite, Parma, ID 83660 USA. [Schreiner, R. Paul] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Lee, J (reprint author), ARS, USDA, PWA, Hort Crops Res Unit Worksite, 29603 U 1 Lane, Parma, ID 83660 USA. EM jungmin.lee@ars.usda.gov; paul.schreiner@ars.usda.gov RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 FU USDA-Agricultural Research Service (ARS); Northwest Center for Small Fruits Research (NCSFR) FX We thank Chris Rennaker and Matthew Scott for technical assistance. This project was primarily funded by USDA-Agricultural Research Service (ARS). The initial set up of the sand-culture vineyard was partially funded by Northwest Center for Small Fruits Research (NCSFR). NR 31 TC 17 Z9 19 U1 2 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2010 VL 119 IS 2 BP 484 EP 489 DI 10.1016/j.foodchem.2009.06.045 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 522BE UT WOS:000271971900009 ER PT J AU Davis, JP Dean, LL Price, KM Sanders, TH AF Davis, J. P. Dean, L. L. Price, K. M. Sanders, T. H. TI Roast effects on the hydrophilic and lipophilic antioxidant capacities of peanut flours, blanched peanut seed and peanut skins SO FOOD CHEMISTRY LA English DT Article DE Peanut; Peanut flour; Peanut skin; Testae; Oxygen radical adsorption capacity; ORAC; Hydrophilic ORAC; Lipophilic ORAC; Antioxidant; Maillard browning ID OLEIC-ACID PEANUTS; VITAMIN-E; FOODS; STABILITY; PHENOLICS; PRODUCTS; STORAGE; DISPERSIONS; EXTRACTION AB Hydrophilic and lipophilic oxygen radical antioxidant capacity (H&L-ORAC) of peanut flours, blanched peanut seed, and peanut skins were characterised across a range of roast intensities. H-ORAC ranged from 5910 to 7990, 3040 to 3700 and 152,290 to 209,710 mu moles Trolox/100 g for the flours, seed, and skins, respectively. H-ORAC increased linearly with darker seed colour after roasting at 166 degrees C from 0 to 77 min, whereas skin H-ORAC peaked after roasting for 7 min. Linear correlations with H-ORAC and total phenolic content were observed. Additionally, completely defatted peanut seed were solubilised (5% w/w) in water and H-ORAC measured. For these samples, H-ORAC decreased with roast intensity which correlated with soluble protein. L-ORAC ranged from 620 to 1120, 150 to 730 and 2150 to 6320 mu moles Trolox/100 g for peanut flours, seed, and skins, respectively. L-ORAC increased linearly with both darker seed colour and skin colour across the 77 min range. L-ORACs of roasted peanuts and ingredients are discussed in terms of tocopherol contents and Maillard reaction products. (C) Published by Elsevier Ltd. C1 [Davis, J. P.; Dean, L. L.; Price, K. M.; Sanders, T. H.] USDA ARS, Market Qual & Handling Res Unit, Raleigh, NC 27695 USA. [Davis, J. P.; Dean, L. L.; Sanders, T. H.] N Carolina State Univ, Dept Food Bioproc & Nutr Serv, Raleigh, NC 27695 USA. RP Davis, JP (reprint author), USDA ARS, Market Qual & Handling Res Unit, Raleigh, NC 27695 USA. EM jack.davis@ars.usda.gov RI Dean, Lisa/B-1463-2015 OI Dean, Lisa/0000-0002-2407-9548 NR 36 TC 18 Z9 22 U1 3 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2010 VL 119 IS 2 BP 539 EP 547 DI 10.1016/j.foodchem.2009.06.057 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 522BE UT WOS:000271971900017 ER PT J AU Hassan, SM Haq, AU Byrd, JA Berhow, MA Cartwright, AL Bailey, CA AF Hassan, S. M. Haq, A. U. Byrd, J. A. Berhow, M. A. Cartwright, A. L. Bailey, C. A. TI Haemolytic and antimicrobial activities of saponin-rich extracts from guar meal SO FOOD CHEMISTRY LA English DT Article DE Bacteria; Guar meal; HPLC; Haemolytic; Saponin ID TRITERPENOID SAPONINS; ALFALFA SAPONINS; PLANT-EXTRACT; IDENTIFICATION; SALMONELLA; MECHANISM; CHICKENS; POULTRY; ACIDS AB Saponin-rich GM extract was prepared by refluxing 25 g of GM with 250 ml of EtOH/H(2)O (1:1, v/v) for 3 h then filtering and distilling EtOH at 50 degrees C, The refluxed extract was partitioned with equal volume of BuOH obtaining crude saponin-rich GM extract with 4.8 +/- 0.6% DM of GM that was purified by RP-HPLC eluting 20%, 60% and 100% MeOH fractions with 2.04 +/- 0.32%. 0.91 +/- 0.16% and 1.55 +/- 0.15% DM of crude saponin-rich GM extract, respectively. Further Purification of 100% MeOH fraction using NP-HPLC eluted four peak sub-fractions at 16, 39, 44 and 46 min. All saponin-rich GM fractions eluted were evaluated for both haemolytic and antimicrobial activities using 96-well plates in eight concentrations. Results indicated that only 100% MeOH fraction and its 16 min peak sub-fraction exhibited both haemolytic and antibacterial activities against Staphylococcus aureus, Salmonella Typhimurium and Escherichia coli, but 20% and 60% MeOH fractions stimulated Lactobacillus spp. growth. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Hassan, S. M.] Suez Canal Univ, Biotechnol Res Ctr, Ismailia, Egypt. [Haq, A. U.; Cartwright, A. L.; Bailey, C. A.] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. [Byrd, J. A.] USDA, Agr Res Ctr, College Stn, TX 77843 USA. [Berhow, M. A.] USDA, Agr Res Ctr, Peoria, IL 61604 USA. RP Hassan, SM (reprint author), Suez Canal Univ, Biotechnol Res Ctr, Ismailia, Egypt. EM sherifz@hotmail.com NR 33 TC 19 Z9 20 U1 0 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2010 VL 119 IS 2 BP 600 EP 605 DI 10.1016/j.foodchem.2009.06.066 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 522BE UT WOS:000271971900026 ER PT J AU Paucar-Menacho, LM Berhow, MA Mandarino, JMG de Mejia, EG Chang, YK AF Paucar-Menacho, Luz Maria Berhow, Mark A. Gontijo Mandarino, Jose Marcos de Mejia, Elvira Gonzalez Chang, Yoon Kil TI Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology SO FOOD CHEMISTRY LA English DT Article DE Soybean; Germination; Soy peptides; Lunasin; Bowman-Birk inhibitor; Lectin; Isoflavone; Saponin ID LUNASIN; SEEDS; PROTEIN AB The objective was to optimise the effect of germination time and temperature on the concentration of soluble protein. lunasin, 13131, lectin, saponins and isoflavones in soybean seeds from cultivar BRS 133. Isoflavone and saponin concentrations were analysed by HPLC. Lunasin, Bowman-Birk inhibitor and lectin were analysed by ELISA and western blot. The effects of the variations in germination time and temperature on bioactive Compounds were analysed using the response surface methodology (RSM), with a 2(2) central composite rotational design. Germination of soybean for 42 h at 25 degrees C resulted in an increase of 61.7% of lunasin, decrease of 58.7% in lectin and 70.0% in lipoxygenase activity. Optimal increases in the concentrations of isoflavone aglycones were observed in combination of 63 h of germination and 30 degrees C. A significant increase of 32.2% in the concentration of soy saponins was observed in combination of 42 h of germination at 25 degrees C. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Paucar-Menacho, Luz Maria; de Mejia, Elvira Gonzalez] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Paucar-Menacho, Luz Maria; Chang, Yoon Kil] Univ Estadual Campinas, Dept Food Technol, Fac Food Engn, Campinas, SP, Brazil. [Berhow, Mark A.] ARS, USDA, Peoria, IL USA. [Gontijo Mandarino, Jose Marcos] Brazilian Agr Res Corp, Soybean Res Lab, Londrina, Brazil. RP de Mejia, EG (reprint author), 228 ERML,MC 051,1201 W Gregory Dr, Urbana, IL 61801 USA. EM edemejia@uiuc.edu FU CAPES-PEC PG; SoSoja do Brasil Ltda.; Sun Foods-Brazil; Embrapa Technology Transfer, Brazil; Unicamp's Foundation for Teaching, Research and Extension (FAEPEX) [149/08] FX The authors wish to thank CAPES-PEC PG for Granting Luz Maria Paucar-Menacho's scholarship and to thanks Rodolfo Rohr Neto (SoSoja do Brasil Ltda.) and Kenji S. Narumiya (Sun Foods-Brazil) for the financial support. Embrapa Soybean - The National Center for Soybean Research, Brazil. Embrapa Technology Transfer, Brazil, for the donation of soybean BRS 133. Unicamp's Foundation for Teaching, Research and Extension (FAEPEX) Grant No. 149/08. NR 16 TC 24 Z9 26 U1 0 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2010 VL 119 IS 2 BP 636 EP 642 DI 10.1016/j.foodchem.2009.07.011 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 522BE UT WOS:000271971900032 ER PT J AU Wondzell, SM Gooseff, MN McGlynn, BL AF Wondzell, Steven M. Gooseff, Michael N. McGlynn, Brian L. TI An analysis of alternative conceptual models relating hyporheic exchange flow to diel fluctuations in discharge during baseflow recession SO HYDROLOGICAL PROCESSES LA English DT Article DE hyporheic; evapotranspiration; stream discharge; riparian; diel fluctuations ID STREAMS; OREGON; USA AB Diel fluctuations in stream flow during baseflow have been observed in many streams and are typically attributed to water losses from evapotranspiration (ET). However, there is no widely transferable conceptual model that explains how ET results in diel fluctuations in streamflow at the watershed outlet. For fluctuations to occur, two factors must be present: (1) some process must generate the fluctuations and transfer them to the stream channel, and (2) fluctuations must be accumulated and transported down the stream network in such a way that they arrive at a stream gauge as a coherent signal. We have previously shown how stream flow velocity affects the transport of diel fluctuations in discharge through a stream network. Here, we examined how riparian ET and hyporheic exchange flows generate diel fluctuations in discharge. We hypothesized that ET would cause a slight drawdown of riparian aquifers during the day, slightly increasing head gradients away from the stream and slightly reducing head gradients back to the stream. Thus, slightly more water would flow into the hyporheic zone than is returned to the stream, gradually reducing stream discharge. The process would be reversed at night. Using stream-tracer experiments and riparian water-level data, we tested two hypotheses related to this conceptual model-that the amplitude (H1) and time lag (H2) of diel aquifer drawdown would be constant over the summer. Neither hypothesis was supported by our data. We conclude that the processes that link watershed ET with streams include both local- and watershed-scale effects. Conceptual models attempting to explain diel fluctuations need to include the combined effects of ET on lateral inputs and hyporheic exchange flows, the redistribution of water within riparian aquifers, and the transport of ET signals from the whole stream network to the stream gauge. Copyright (C) 2009 John Wiley & Sons, Ltd. C1 [Wondzell, Steven M.] USDA Forest Serv, Pacific NW Res Stn, Olympia Forestry Sci Lab, Olympia, WA 98512 USA. [Gooseff, Michael N.] Penn State Univ, Dept Civil & Environm Engn, State Coll, PA 16802 USA. [McGlynn, Brian L.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. RP Wondzell, SM (reprint author), USDA Forest Serv, Pacific NW Res Stn, Olympia Forestry Sci Lab, Olympia, WA 98512 USA. EM swondzell@fs.fed.us RI Gooseff, Michael/B-9273-2008; McGlynn, Brian/A-2509-2008; Gooseff, Michael/N-6087-2015 OI McGlynn, Brian/0000-0001-5266-4894; Gooseff, Michael/0000-0003-4322-8315 FU National Science Foundation's (NSF) Hydrologic Sciences Program (NSF) [EAR-9506669, EAR-9909564, EAR-0530873, EAR-0337650, BSR-9011663, DEB-9632921]; USDA Forest Service, Pacific Northwest Research Station FX We thank Barbara Bond for sharing estimates of evapotranspiration based on sapflow measurements made in WS1. We thank Willem VanVerseveld for sharing data collected from the WS1 well network over the summer of 2004. We thank Dominique Bachelet, Ken Bencala, Kevin McGuire, Willem VanVerseveld, Julia Jones, Georgianne Moore, and Nobi Suzuki for discussion of the ideas and for review of the text, all of which have improved the manuscript. This work was supported by the National Science Foundation's (NSF) Hydrologic Sciences Program (NSF Grant numbers EAR-9506669, EAR-9909564, EAR-0530873, and EAR-0337650) and by the USDA Forest Service, Pacific Northwest Research Station's Aquatic and Land Interactions Programs.; Climate and stream discharge data were provided by the Forest Science Data Bank, a partnership between the Department of Forest Science, Oregon State University, and the US Forest Service Pacific Northwest Research Station, Corvallis, Oregon. Significant funding for these data was provided by the NSF Long-Term Ecological Research Program (NSF Grant numbers BSR-9011663 and DEB-9632921). NR 20 TC 25 Z9 25 U1 1 U2 24 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0885-6087 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 15 PY 2010 VL 24 IS 6 BP 686 EP 694 DI 10.1002/hyp.7507 PG 9 WC Water Resources SC Water Resources GA 576QL UT WOS:000276161000002 ER PT J AU Kim, SC Davis, JG Truman, CC Ascough, JC Carlson, K AF Kim, Sung-Chul Davis, Jessica G. Truman, Clinton C. Ascough, James C., II Carlson, Kenneth TI Simulated rainfall study for transport of veterinary antibiotics - mass balance analysis SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Veterinary antibiotics; HPLC/MS/MS; Rainfall simulation; Transport; Mass balance ID WASTE-WATER; LIQUID-CHROMATOGRAPHY; PHARMACEUTICAL ANTIBIOTICS; PESTICIDE TRANSPORT; RESISTANCE GENES; TREATMENT PLANTS; SOIL; SORPTION; TETRACYCLINE; SURFACE AB Occurrence of human and veterinary antibiotics has been reported in various environmental compartments. Yet, there is a lack of information verifying the transport mechanisms from source to environment, particularly the transport of veterinary antibiotics as a non-point source pollutant. A rainfall simulation study was conducted to address surface runoff as a possible transport mechanism of veterinary antibiotics introduced in the field and mass balance was calculated with supplementary surface and depth soil measurement. Seven veterinary antibiotics that are the most abundantly used in agriculture for therapeutic and non-therapeutic (growth-promotion) purposes were examined in this study, including tetracycline (TC), chlortetracycline (CTC), sulfathiazole (STZ). sulfamethazine (SMZ), erythromycin (ETM). tylosin (TYL), and monensin (MNS). Runoff in aqueous and sediment phases was collected every 5 min for I h with varied rainfall intensity and additional surface (0-2 cm) and depth (2-30 cm) soil samples were collected after rainfall simulation for mass balance analysis. Quantification of antibiotic concentration in all collected samples was based on solid phase extraction (SPE) followed by measurement with high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). MNS showed the highest concentration in runoff aqueous samples (0.22 mg plot(-1)),while ETM showed the highest concentration in runoff sediment samples (0.08 mg plot(-1)). The highest concentration of each applied antibiotic in surface soil samples occurred at different locations. This result might indicate the mobility of these compounds in surface soil varies due to different physicochemical properties among the antibiotics. Further, the analysis results showed that all of the subject antibiotics had penetrated into the subsurface; yet, no residuals were found for STZ, suggesting this compound might have penetrated even deeper into the soil. These results indicate that aqueous or sediment erosion control might reduce the transport of veterinary antibiotics in the environment. (C) 2009 Elsevier B.V, All rights reserved. C1 [Kim, Sung-Chul; Carlson, Kenneth] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Davis, Jessica G.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Truman, Clinton C.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. [Ascough, James C., II] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. RP Carlson, K (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. EM sckimenv@kangwon.ac.kr; jessica.Davis@Colostate.edu; clint.truman@ars.usda.gov; jim.ascough@ars.usda.gov; kcarlson@engr.colostate.edu RI Davis, Jessica/C-1903-2013; feng, yongzhong/F-5090-2012 OI feng, yongzhong/0000-0002-5202-4368 FU USDA FX The authors are grateful for the expertise and assistance of Donald Dick in the Department of Chemistry at Colorado State University. This project was funded by a grant from the USDA National Water Quality Integrated Program. NR 45 TC 30 Z9 31 U1 4 U2 59 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 J9 J HAZARD MATER JI J. Hazard. Mater. PD MAR 15 PY 2010 VL 175 IS 1-3 BP 836 EP 843 DI 10.1016/j.jhazmat.2009.10.086 PG 8 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 555EI UT WOS:000274490400106 PM 19932562 ER PT J AU Dragull, K Beck, JJ Merrill, GB AF Dragull, Klaus Beck, John J. Merrill, Glory B. TI Essential oil yield and composition of Pistacia vera 'Kerman' fruits, peduncles and leaves grown in California SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE essential oil; Kerman; limonene; peduncles; Pistacia vera; terpinolene; thujene ID VOLATILE; EMISSION; ALMONDS; L. AB BACKGROUND: Pistacia vera 'Kerman' is the predominant pistachio nut cultivar in the United States (California), the world's second largest producer. Despite several reports on the essential oil (EO) content in the genus Pistacia, data on 'Kerman' are limited. The EO content and volatile organic compound (VOC) emissions of tree nut orchards are of current interest to researchers investigating insect pests and the potential role of EO and VOCs as semiochemicals. To establish a basis for the VOC output of pistachios, the EO content of fruits, peduncles, and leaves was analyzed. RESULTS: Evaluated plant parts contained limonene as the primary EO component, followed by alpha-terpinolene. Peduncles were unique in containing relatively high levels of alpha-thujene. The results were reproducible between two different geographical locations. In situ solid phase microextraction (SPME) studies demonstrated the volatile emission was representative of the EO composition. CONCLUSION: This is the first report detailing the content and distribution of EO and the unique limonene-dominant profile for this Pistacia vera cultivar which may influence pistachio insect pest semiochemical research. (C) 2010 Society of Chemical Industry C1 [Dragull, Klaus; Beck, John J.; Merrill, Glory B.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Dragull, K (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM klaus.dragull@ars.usda.gov FU USDA-ARS CRIS [5325-42000-036-00] FX This research was conducted under USDA-ARS CRIS project 5325-42000-036-00. The authors thank B.S. Higbee, J. Battig, W.S. Gee and J. Edstrom for their assistance. NR 24 TC 11 Z9 11 U1 3 U2 22 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD MAR 15 PY 2010 VL 90 IS 4 BP 664 EP 668 DI 10.1002/jsfa.3865 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 562DA UT WOS:000275028300018 PM 20355096 ER PT J AU Wickham, JD Riitters, KH Wade, TG Vogt, P AF Wickham, James D. Riitters, Kurt H. Wade, Timothy G. Vogt, Peter TI A national assessment of green infrastructure and change for the conterminous United States using morphological image processing SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Conservation; Corridors; Ecological networks; Land-cover change; Landscape ecology; Restoration ID LAND-COVER DATABASE; FOREST FRAGMENTATION; ECOLOGICAL NETWORKS; LANDSCAPE CORRIDORS; TEMPORAL-CHANGE; CONSERVATION; INDICATORS; SCALES; TERRESTRIAL; COMPLETION AB Green infrastructure is a popular framework for conservation planning. The main elements of green infrastructure are hubs and links. Hubs tend to be large areas of 'natural' vegetation and links tend to be linear features (e.g., streams) that connect hubs. Within the United States, green infrastructure projects can be characterized as: (1) reliant on classical geographic information system (GIS) techniques (e.g., overlay, buffering) for mapping: (2), mainly implemented by states and local jurisdictions; and (3) static assessments that do not routinely incorporate information on land-cover change. We introduce morphological spatial pattern analysis (MSPA) as a complementary way to map green infrastructure, extend the geographic scope to the conterminous United States, and incorporate land-cover change information. MSPA applies a series of image processing routines to a raster land-cover map to identify hubs, links, and related structural classes of land cover. We identified approximately 4000 large networks (>100 hubs) within the conterminous United States, of which approximately 10% crossed state boundaries. We also identified a net loss of up to 3.59 million ha of links and 1.72 million ha of hubs between 1992 and 2001. Our national assessment provides a backbone that states could use to coordinate their green infrastructure projects, and our incorporation of change illustrates the importance of land-cover dynamics for green infrastructure planning and assessment. Published by Elsevier B.V. C1 [Wickham, James D.; Wade, Timothy G.] US EPA, Off Res & Dev, Natl Exposure Res Lab MD E243 05, Res Triangle Pk, NC 27711 USA. [Riitters, Kurt H.] US Forest Serv, So Res Stn, Res Triangle Pk, NC 27709 USA. [Vogt, Peter] Commiss European Communities, Joint Res Ctr, Inst Environm & Sustainabil, I-21020 Ispra, VA, Italy. RP Wickham, JD (reprint author), US EPA, Off Res & Dev, Natl Exposure Res Lab MD E243 05, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM wickham.james@epa.gov FU US Environmental Protection Agency (EPA); Office of Research and Development (ORD) FX The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded the research. This manuscript has been subjected to the EPA's peer and administrative review, and it has been approved for publication. The authors thank Christine Mazzarella for her contributions to the project. NR 55 TC 38 Z9 45 U1 10 U2 92 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAR 15 PY 2010 VL 94 IS 3-4 BP 186 EP 195 DI 10.1016/j.landurbplan.2009.10.003 PG 10 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 560ER UT WOS:000274885300005 ER PT J AU Mastro, LL Conover, MR Frey, SN AF Mastro, Lauren L. Conover, Michael R. Frey, S. Nicole TI Factors influencing a motorist's ability to detect deer at night SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Deer-vehicle collisions; Roads; Vehicle accidents ID VEHICLE ACCIDENTS; RECOGNITION; PEDESTRIANS; HIGHWAY AB Most deer-vehicle collisions (DVCs) occur at night when deer are active and the ability of motorists to see them is impaired. The objective of this study was to examine the ability of motorists to detect deer at night and examine select factors which may influence detection distances. We examined the ability of motorists to detect deer at night and the factors that influence detection using deer decoys, volunteer motorists, and a series of driving courses. Deer decoys were detected at significantly greater distances when located on the right rather than on the left side of the road and when <10m from the road's edge. Detection distances were significantly longer for high-beam headlights versus low-beam headlights. Moving decoys were detected at similar distances to stationary ones. Additionally, decoys were detected at greater distances in the absence of reflectors than in their presence. The frequency of nocturnal DVCs can be reduced by using high-beam headlamps and minimizing the number of roadside reflectors, signs, and other bright objects that distract the driver's attention. (C) 2009 Elsevier B.V. All rights reserved. C1 [Mastro, Lauren L.; Conover, Michael R.; Frey, S. Nicole] Utah State Univ, Jack H Berryman Inst, Dept Wildland Resources, Logan, UT 84322 USA. RP Mastro, LL (reprint author), USDA, APHIS, WS, NWRC, 730 Yokum St, Elkins, WV 26241 USA. EM Lauren.L.Mastro@aphis.usda.gov FU Jack H. Berryman Institute; Utah Agricultural Experiment Station FX The Jack H. Berryman Institute and the Utah Agricultural Experiment Station provided funding for this study. We thank numerous study participants, without whom this study would not have been possible. Special thanks to J. Borgo and K. Sivey for their help conducting these tests. NR 18 TC 8 Z9 8 U1 5 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAR 15 PY 2010 VL 94 IS 3-4 BP 250 EP 254 DI 10.1016/j.landurbplan.2009.10.010 PG 5 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 560ER UT WOS:000274885300012 ER PT J AU Xiao, JF Zhuang, QL Law, BE Chen, JQ Baldocchi, DD Cook, DR Oren, R Richardson, AD Wharton, S Ma, SY Martin, TA Verma, SB Suyker, AE Scott, RL Monson, RK Litvak, M Hollinger, DY Sun, G Davis, KJ Bolstad, PV Burns, SP Curtis, PS Drake, BG Falk, M Fischer, ML Foster, DR Gu, LH Hadley, JL Katul, GG Roser, Y McNulty, S Meyers, TP Munger, JW Noormets, A Oechel, WC Paw, KT Schmid, HP Starr, G Torn, MS Wofsy, SC AF Xiao, Jingfeng Zhuang, Qianlai Law, Beverly E. Chen, Jiquan Baldocchi, Dennis D. Cook, David R. Oren, Ram Richardson, Andrew D. Wharton, Sonia Ma, Siyan Martin, Timothy A. Verma, Shashi B. Suyker, Andrew E. Scott, Russell L. Monson, Russell K. Litvak, Marcy Hollinger, David Y. Sun, Ge Davis, Kenneth J. Bolstad, Paul V. Burns, Sean P. Curtis, Peter S. Drake, Bert G. Falk, Matthias Fischer, Marc L. Foster, David R. Gu, Lianhong Hadley, Julian L. Katul, Gabriel G. Matamala, Roser McNulty, Steve Meyers, Tilden P. Munger, J. William Noormets, Asko Oechel, Walter C. Paw U, Kyaw Tha Schmid, Hans Peter Starr, Gregory Torn, Margaret S. Wofsy, Steven C. TI A continuous measure of gross primary production for the conterminous United States derived from MODIS and AmeriFlux data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Gross primary productivity; MODIS; AmeriFlux; Eddy covariance; Regression tree; US; Carbon fluxes; Interannual variability; Satellite data; Biomes ID CARBON-DIOXIDE EXCHANGE; NET ECOSYSTEM EXCHANGE; EDDY-COVARIANCE MEASUREMENTS; ELEVATED ATMOSPHERIC CO2; DIFFERENCE WATER INDEX; NORTHERN GREAT-PLAINS; LIGHT-USE EFFICIENCY; FOREST ECOSYSTEMS; PONDEROSA PINE; DECIDUOUS FOREST AB The quantification of carbon fluxes between the terrestrial biosphere and the atmosphere is of scientific importance and also relevant to climate-policy making. Eddy covariance flux towers provide continuous measurements of ecosystem-level exchange of carbon dioxide spanning diurnal, synoptic, seasonal, and interannual time scales. However, these measurements only represent the fluxes at the scale of the tower footprint. Here we used remotely sensed data from the Moderate Resolution Imaging Spectroradiometer (MODIS) to upscale gross primary productivity (GPP) data from eddy covariance flux towers to the continental scale. We first combined GPP and MODIS data for 42 AmeriFlux towers encompassing a wide range of ecosystem and climate types to develop a predictive GPP model using a regression tree approach. The predictive model was trained using observed GPP over the period 2000-2004, and was validated using observed GPP over the period 2005-2006 and leave-one-out cross-validation. Our model predicted GPP fairly well at the site level. We then used the model to estimate GPP for each I km x I km cell across the U.S. for each 8-day interval over the period from February 2000 to December 2006 using MODIS data. Our GPP estimates provide a spatially and temporally continuous measure of gross primary production for the U.S. that is a highly constrained by eddy covariance flux data. Our study demonstrated that our empirical approach is effective for upscaling eddy flux GPP data to the continental scale and producing continuous GPP estimates across multiple biomes. With these estimates, we then examined the patterns, magnitude, and interannual variability of GPP. We estimated a gross carbon uptake between 6.91 and 7.33 Pg C yr(-1) for the conterminous U.S. Drought, fires, and hurricanes reduced annual GPP at regional scales and could have a significant impact on the U.S. net ecosystem carbon exchange. The sources of the interannual variability of U.S. GPP were dominated by these extreme climate events and disturbances. (C) 2009 Elsevier Inc. All rights reserved. C1 [Xiao, Jingfeng] Purdue Univ, Purdue Climate Change Res Ctr, Dept Earth & Atmospher Sci, W Lafayette, IN 47907 USA. [Zhuang, Qianlai] Purdue Univ, Purdue Climate Change Res Ctr, Dept Earth & Atmospher Sci, Dept Agron, W Lafayette, IN 47907 USA. [Law, Beverly E.] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Chen, Jiquan] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Baldocchi, Dennis D.; Ma, Siyan] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Ecosyst Sci Div, Berkeley, CA 94720 USA. [Cook, David R.] Argonne Natl Lab, Div Environm Sci, Argonne, IL 60439 USA. [Oren, Ram; Katul, Gabriel G.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Wharton, Sonia; Falk, Matthias; Paw U, Kyaw Tha] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Martin, Timothy A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Verma, Shashi B.; Suyker, Andrew E.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Scott, Russell L.] USDA ARS, SW Watershed Res Ctr, Tucson, AZ 85719 USA. [Monson, Russell K.; Burns, Sean P.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Litvak, Marcy] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Hollinger, David Y.] US Forest Serv, USDA, NE Res Stn, Durham, NH 03824 USA. [Sun, Ge; McNulty, Steve] US Forest Serv, USDA, So Res Stn, Raleigh, NC 27606 USA. [Davis, Kenneth J.] Univ New Hampshire, Complex Syst Res Ctr, Durham, NH 03824 USA. [Bolstad, Paul V.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Curtis, Peter S.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Drake, Bert G.] Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA. [Fischer, Marc L.] Univ Calif Berkeley, Lawrence Berkeley Lab, Environm Energy Technol Div, Dept Atmospher Sci, Berkeley, CA 94720 USA. [Foster, David R.] Harvard Univ, Dept Organism & Evolutionary Biol, Petersham, MA 01366 USA. [Gu, Lianhong] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Matamala, Roser] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA. [Meyers, Tilden P.] NOAA, ARL, Atmospher Turbulence & Diffus Div, Oak Ridge, TN 37831 USA. [Wofsy, Steven C.] Harvard Univ, Dept Earth & Planetary Sci, Div Engn & Appl Sci, Cambridge, MA 02138 USA. [Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Noormets, Asko] N Carolina State Univ, So Global Change Program, Raleigh, NC 27695 USA. [Oechel, Walter C.] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Schmid, Hans Peter] Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. [Schmid, Hans Peter] FZK IMK IFU, Res Ctr Karlsruhe, Inst Meteorol & Climate Res, D-82467 Garmisch Partenkirchen, Germany. [Starr, Gregory] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Torn, Margaret S.] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Earth Sci, Berkeley, CA 94720 USA. RP Xiao, JF (reprint author), Penn State Univ, Dept Meteorol, 503 Walker Bldg, University Pk, PA 16802 USA. EM j.xiao@unh.edu RI Munger, J/H-4502-2013; Oechel, Walter/F-9361-2010; Gu, Lianhong/H-8241-2014; Hollinger, David/G-7185-2012; Schmid, Hans Peter/I-1224-2012; Chen, Jiquan/D-1955-2009; Noormets, Asko/A-7257-2009; Katul, Gabriel/A-7210-2008; Garmisch-Pa, Ifu/H-9902-2014; Baldocchi, Dennis/A-1625-2009; Torn, Margaret/D-2305-2015; Meyers, Tilden/C-6633-2016; Richardson, Andrew/F-5691-2011 OI Munger, J/0000-0002-1042-8452; Oechel, Walter/0000-0002-3504-026X; Gu, Lianhong/0000-0001-5756-8738; Law, Beverly/0000-0002-1605-1203; Martin, Timothy/0000-0002-7872-4194; Schmid, Hans Peter/0000-0001-9076-4466; Noormets, Asko/0000-0003-2221-2111; Katul, Gabriel/0000-0001-9768-3693; Baldocchi, Dennis/0000-0003-3496-4919; Richardson, Andrew/0000-0002-0148-6714 FU National Science Foundation (NSF) [EAR-0630319] FX This research was partly supported by the National Science Foundation (NSF) Carbon and Water Program (EAR-0630319). We thank the principal investigators of the MODIS data products including Drs. A.R. Huete, R.B. Myneni, S.W. Running, E.F. Vermote, and Z. Wan. We also thank D.A. Sims, T.A. Boden, S.K.S. Vannan, M. Zhao, Z. Wan, and L Delp for helpful discussion. The MODIS data products were obtained from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC) and the Earth Observing System (EOS) Data Gateway for making these products available. The PRISM climate database was provided by the PRISM Group, Oregon State University (http://www.prismclimate.org). NR 102 TC 112 Z9 119 U1 8 U2 65 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 15 PY 2010 VL 114 IS 3 BP 576 EP 591 DI 10.1016/j.rse.2009.10.013 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 559JP UT WOS:000274820700012 ER PT J AU Krikorian, R Nash, TA Shidler, MD Shukitt-Hale, B Joseph, JA AF Krikorian, Robert Nash, Tiffany A. Shidler, Marcelle D. Shukitt-Hale, Barbara Joseph, James A. TI Concord grape juice supplementation improves memory function in older adults with mild cognitive impairment SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Memory; Cognitive impairment; Elderly; Grape juice; Polyphenols ID BLOOD-BRAIN-BARRIER; ALZHEIMERS-DISEASE; BLUEBERRY SUPPLEMENTATION; PERISPINAL ETANERCEPT; OXIDATIVE STRESS; FLAVONOIDS; DEMENTIA; ANTHOCYANINS; PERFORMANCE; FRUIT AB Concord grape juice contains polyphenol compounds, which have antioxidant and anti-inflammatory properties and influence neuronal signalling. Concord grape juice supplementation has been shown to reduce inflammation, blood pressure and vascular pathology in individuals with CVD, and consumption of such flavonoid-containing foods is associated with a reduced risk for dementia. In addition, preliminary animal data have indicated improvement in memory and motor function with grape juice supplementation, suggesting potential for cognitive benefit in ageing humans. In this initial investigation of neurocognitive effects, we enrolled twelve older adults with memory decline but not dementia in a randomised, placebo-controlled, double-blind trial with Concord grape juice supplementation for 12 weeks. We observed significant improvement in a measure of verbal learning and non-significant enhancement of verbal and spatial recall. There was no appreciable effect of the intervention on depressive symptoms and no effect on weight or waist circumference. A small increase in fasting insulin was observed for those consuming grape juice. These preliminary findings suggest that supplementation with Concord grape juice may enhance cognitive function for older adults with early memory decline and establish a basis for more comprehensive investigations to evaluate potential benefit and assess mechanisms of action. C1 [Krikorian, Robert; Nash, Tiffany A.; Shidler, Marcelle D.] Univ Cincinnati, Acad Hlth Ctr, Dept Psychiat, Cincinnati, OH 45221 USA. [Shukitt-Hale, Barbara; Joseph, James A.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Krikorian, R (reprint author), Univ Cincinnati, Acad Hlth Ctr, Dept Psychiat, Cincinnati, OH 45221 USA. EM robert.krikorian@uc.edu FU Welch Foods, Inc. (Concord, MA, USA) FX Funding and material support was provided by Welch Foods, Inc. (Concord, MA, USA). NR 55 TC 106 Z9 110 U1 10 U2 53 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 J9 BRIT J NUTR JI Br. J. Nutr. PD MAR 14 PY 2010 VL 103 IS 5 BP 730 EP 734 DI 10.1017/S0007114509992364 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 571CV UT WOS:000275729800012 PM 20028599 ER PT J AU Li, RP Zhang, Y Lee, CC Lu, RR Huang, YP AF Li, Ruiping Zhang, Yi Lee, Charles C. Lu, Rongrong Huang, Yingping TI Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article DE Hydrophilic interaction liquid chromatography (HILIC); Aromatic amines; Solid-phase extraction (SPE); Water analysis ID SOLID-PHASE EXTRACTION; TANDEM MASS-SPECTROMETRY; GAS-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; HUMAN PLASMA; WASTE-WATER; ELECTROCHEMICAL DETECTION; IONIZABLE COMPOUNDS; MONOLITHIC COLUMN; SAMPLES AB A simple, precise, and accurate hydrophilic interaction liquid chromatographic (HILIC) method has been developed for the determination of five aromatic amines in environmental water samples Chromatography was carried out on a bare silica column, using a mixture of acetonitrile and a buffer of NaH(2)PO(4)-H(3)PO(4) (pH 1 5, containing 10 mM NaH(2)PO(4)) (85.15. v/v) as a mobile phase at a flow rate of 1 mL mm(-1). Aromatic amines were detected by UV absorbance at 254 nm. The linear range of amines was good (r(2) > 0 998) and limit of detection (LOD) within 0.02-0.2 mg L(-1) (S/N = 3). The retention mechanism for the analytes under the optimum conditions was determined to be a combination of adsorption. partition and ionic interactions The proposed method was applied to the environmental water samples Aromatic amines were Isolated aqueous samples using solid-phase extraction (SPE) with Oasis HLB cartridges Recoveries of greater than 75% with precision (IRSID) less than 12% were obtained at amine concentrations of 5-50 mu g L(-1) ;from 100 mL river water and influents from a wastewater treatment plant (WWTP). The present HILIC technique proved to be a viable method for the analysis of aromatic amines in the environmental water C1 [Li, Ruiping; Zhang, Yi; Lu, Rongrong; Huang, Yingping] China Three Georges Univ, Engn Res Ctr Ecoenvironm Gorges Reservoir Reg 3, Minist Educ, Yichang 443002, Peoples R China. [Lee, Charles C.] USDA ARS WRRC, Albany, CA 94710 USA. RP Li, RP (reprint author), China Three Georges Univ, Engn Res Ctr Ecoenvironm Gorges Reservoir Reg 3, Minist Educ, Yichang 443002, Peoples R China. FU NSFC [20877048]; National Basic Research Program (973) of China [2008CB417206] FX This work was supported by NSFC (No 20877048) and the National Basic Research Program (973) of China (No. 2008CB417206) Also, we greatly appreciate Senior Experimentalists Bailing Chen and Quanbin Liao, College of Chemistry and Life Science, CTGU for providing some reagents NR 61 TC 23 Z9 31 U1 5 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR A JI J. Chromatogr. A PD MAR 12 PY 2010 VL 1217 IS 11 BP 1799 EP 1805 DI 10.1016/j.chroma.2010.01.049 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 566EV UT WOS:000275353800010 PM 20149379 ER PT J AU Norrbom, AL Sutton, BD Steck, GJ Monzon, J AF Norrbom, Allen L. Sutton, Bruce D. Steck, Gary J. Monzon, Jose TI New genera, species and host plant records of Nearctic and Neotropical Tephritidae (Diptera) SO ZOOTAXA LA English DT Article DE Diptera; Tephritidae; Tephritinae; taxonomy; host plant; Asteraceae ID IMMATURE STAGES; SOUTHERN-CALIFORNIA; LIFE-HISTORY; ASTERACEAE; BIOLOGY AB Three new genera and five new species of Tephritidae (Diptera) are described from the Nearctic and Neotropical Regions. The new genera are: Agallamyia Norrbom (type species: A. pendula Norrbom, n. sp.), Neosphaeniscus Norrbom (type species: Euribia m-nigrum Hendel), and Phacelochaeta Norrbom (type species: Procecidochares quinquefasciata Hendel). The new species include: Acidogona stecki Norrbom (Guatemala, Mexico: Chiapas), Agallamyia pendula Norrbom (Guatemala), Phacelochaeta obliqua Norrbom (Ecuador), Procecidochares suttoni Norrbom (Guatemala), Stenopa mexicana Norrbom (Mexico). Five new generic synonyms are proposed: Cecidocharella Hendel, 1936 = Dracontomyia Becker, 1919; Gerrhoceras Hering, 1942 = Pyrgotoides Curran, 1934; Stoneola Hering, 1941 = Rhagoletis Loew, 1862; Strobelia Rondani, 1868 = Rachiptera Bigot, 1859; and Xenochaeta Snow, 1894 = Acidogona Loew, 1873. The following 41 new combinations are proposed: Acidogona dichromata (Snow), Dictyotrypeta crenulata (Wulp), D. incisa (Wulp), Dioxyna crockeri (Curran), Dracontomyia tucumana (Aczel), D. borrichia (Bush & Huettel), and D. elegans (Hendel), Homoeothrix aberrans (Schiner), Neosphaeniscus m-nigrum (Hendel) and N. flexuosus (Bigot), Paracantha trinotata (Foote), Phacelochaeta quinquefasciata (Hendel) and P. quinquevittata (Norrbom), Plaumannimyia ameghinoi (Brethes), P. coelestina (Hering), P. delicatella (Blanchard), P. difficilis (Malloch), P. dolores (Hering), P. eugenia (Wulp), P. flava (Adams), P. hestiae (Hendel), P. imitatrix (Hering), P. miseta (Hering), P. plagiata (Blanchard), P. scutellata (Seguy), P. setulosa (Malloch), P. subaster (Malloch), P. suspecta (Malloch), P. thomsoni (Hendel), P. titschacki (Hering), and P. valdesiana (Gandolfo & Norrbom), Pyrgotoides paradoxus (Hering) and P. peruvianus (Korytkowski), Rachiptera alboguttata (Hendel), R. baccharidis (Rondani), R. bimaculata (Hendel), R. ferruginea (Hendel), R. lutulenta (Hendel), R. parallela (Hendel), and R. rubiginosa (Rondani), and Rhagoletis fuscobasalis (Hering). A lectotype is designated for R. fuscobasalis. New distribution and host plant records also are reported. C1 [Norrbom, Allen L.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, Washington, DC 20013 USA. [Sutton, Bruce D.; Steck, Gary J.] Florida Dept Agr & Consumer Serv, Gainesville, FL 32614 USA. [Monzon, Jose] Univ Valle Guatemala, Lab Entomol Sistemat, Guatemala City 01901, Guatemala. RP Norrbom, AL (reprint author), ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM allen.norrbom@ars.usda.gov; tonb@doacs.state.fl.us; steckg@doacs.state.fl.us; dynastes@intelnett.com NR 43 TC 1 Z9 1 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 12 PY 2010 IS 2398 BP 1 EP 65 PG 65 WC Zoology SC Zoology GA 567NR UT WOS:000275454400001 ER PT J AU Woodley, NE AF Woodley, Norman E. TI Parameristomerinx copelandi-a new genus and species of Afrotropical Pachygastrinae and a new generic synonym of Dolichodema Kertesz (Diptera: Stratiomyidae) SO ZOOTAXA LA English DT Article DE Diptera; Stratiomyidae; Pachygastrinae; Parameristomerinx; Dolichodema; Afrotropical AB A new genus and species, Parameristomerinx copelandi gen. et sp. n. (Diptera: Stratiomyidae: Pachygastrinae) is described from material from Kenya. This genus is similar to the Afrotropical genera Meristomerinx Enderlein, 1914 and Meristomeringina James, 1952. A new generic synonym is also proposed, Nemodema Lindner, 1958 = Dolichodema Kertesz, 1916, syn. n. A key is included for the six genera related to Meristomerinx. C1 ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst NHB 168, Washington, DC 20013 USA. RP Woodley, NE (reprint author), ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst NHB 168, POB 37012, Washington, DC 20013 USA. EM norman.woodley@ars.usda.gov RI Woodley, Norman/M-6160-2014 NR 10 TC 1 Z9 1 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 12 PY 2010 IS 2397 BP 41 EP 47 PG 7 WC Zoology SC Zoology GA 567NQ UT WOS:000275454300004 ER PT J AU Tamborindeguy, C Monsion, B Brault, V Hunnicutt, L Ju, HJ Nakabachi, A Van Fleet, E AF Tamborindeguy, C. Monsion, B. Brault, V. Hunnicutt, L. Ju, H. J. Nakabachi, A. Van Fleet, E. TI A genomic analysis of transcytosis in the pea aphid, Acyrthosiphon pisum, a mechanism involved in virus transmission SO INSECT MOLECULAR BIOLOGY LA English DT Article DE genome annotation; virus transmission; vesicle trafficking; aphid; endocytosis; exocytosis ID WESTERN YELLOWS VIRUS; CLATHRIN-COATED PITS; PROTEIN READTHROUGH DOMAIN; ACTIN-RELATED PROTEINS; MINOR CAPSID PROTEIN; MYZUS-PERSICAE; RAB GTPASES; MEDIATED ENDOCYTOSIS; MEMBRANE-TRANSPORT; SYNAPTOTAGMIN-III AB Aphids are the primary vectors of plant viruses. Transmission can occur via attachment to the cuticle lining of the insect (non-circulative transmission) or after internalization in the insect cells with or without replication (circulative transmission). In this paper, we have focused on the circulative and non-propagative mode during which virions enter the cell following receptor-mediated endocytosis, are transported across the cell in vesicles and released by exocytosis without replicating. The correct uptake, transport and delivery of the vesicles cargo relies on the participation of proteins from different families which have been identified in the Acyrthosiphon pisum genome. Assemblage of this annotated dataset provides a useful basis to improve our understanding of the molecules and mechanisms involved in virus transmission by A. pisum and other aphid species. C1 [Tamborindeguy, C.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Tamborindeguy, C.; Ju, H. J.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Van Fleet, E.] Ithaca Coll, Dept Biol Sci, Ithaca, NY 14850 USA. [Hunnicutt, L.] N Carolina State Univ, Genom Sci Program, Raleigh, NC 27695 USA. [Nakabachi, A.] RIKEN, Adv Sci Inst, Wako, Saitama, Japan. [Monsion, B.; Brault, V.] INRA, UMR SVQV, Colmar, France. RP Tamborindeguy, C (reprint author), Texas A&M Univ, Dept Entomol, TAMU 2475, College Stn, TX 77843 USA. EM ctamborindeguy@ag.tamu.edu RI Nakabachi, Atsushi/H-1661-2011; Tamborindeguy, Cecilia/A-6110-2012; OI Monsion, Baptiste/0000-0002-5174-4742 FU USDA [2005-35604-15446] FX We thank the International Aphid Genomics Consortium and the Baylor College of Medicine Human Genome Sequencing Centre for making the A. pisum genome sequences publicly available prior to publication. Part of this work was funded by USDA grant 2005-35604-15446. NR 90 TC 12 Z9 12 U1 0 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD MAR 10 PY 2010 VL 19 SU 2 BP 259 EP 272 DI 10.1111/j.1365-2583.2009.00956.x PG 14 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 560NK UT WOS:000274909200024 PM 20482656 ER PT J AU Lester, GE Makus, DJ Hodges, DM AF Lester, Gene E. Makus, Donald J. Hodges, D. Mark TI Relationship between Fresh-Packaged Spinach Leaves Exposed to Continuous Light or Dark and Bioactive Contents: Effects of Cultivar, Leaf Size, and Storage Duration SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Spinacia oleracea; antioxidants; ascorbic acid; beta-carotene; folate; lutein; 5-methyltetra-hydrofolate; tocopherol; phylloquinone; postharvest storage; violaxanthin; zeaxanthin ID ASCORBIC-ACID; PHYLLOQUINONE VITAMIN-K-1; POSTHARVEST STORAGE; OLERACEA L.; ANTIOXIDANT CAPACITY; VISUAL QUALITY; PHOTOSYSTEM-I; BABY SPINACH; FOLIC-ACID; BIOSYNTHESIS AB Current retail marketing conditions allow produce to receive artificial light 24 h per day during its displayed shelf life. Essential human-health vitamins [ascorbic acid (vit C), folate (vit B-9), phylloquinone (vit K-1), (alpha-tocopherol (vit E), and the carotenoids lutein, violaxanthin, zeaxanthin, and beta-carotene (provit A)] also are essential for photosynthesis and are biosynthesized in plants by light conditions even under chilling temperatures. Spinach leaves, notably abundant in the aforementioned human-health compounds, were harvested from flat-leaf 'Lazio' and crinkle-leafed 'Samish' cultivars at peak whole-plant maturity as baby (top- and midcanopy) and larger (lower-canopy) leaves. Leaves were placed as a single layer in commercial, clear-polymer retail boxes and stored at 4 degrees C for up to 9 days under continuous light (26.9 mu mol. m(2) .s) or dark. Top-canopy, baby-leaf spinach generally had higher concentrations of all bioactive compounds, on a dry weight basis, with the exception of carotenoids, than bottom-canopy leaves, All leaves stored under continuous light generally had higher levels of all bioactive compounds, except beta-carotene and violaxanthin, and were more prone to wilting, especially the flat-leafed cultivar. All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds. Findings from this study revealed that spinach leaves exposed to simulated retail continuous light at 4 degrees C, in clear plastic containers, were overall more nutritionally dense (enriched) than leaves exposed to continuous darkness. C1 [Lester, Gene E.; Makus, Donald J.] ARS, Kika Garza Subtrop Agr Res Ctr, USDA, Weslaco, TX 78596 USA. [Hodges, D. Mark] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, Kentville, NS B4N 1J5, Canada. RP Lester, GE (reprint author), USDA ARS, 103000 Baltimore Ave,Bldg 002,Rm 113,BARC W, Beltsville, MD 20705 USA. EM lester@ars.usda.gov NR 38 TC 29 Z9 31 U1 1 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 10 PY 2010 VL 58 IS 5 BP 2980 EP 2987 DI 10.1021/jf903596v PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 562WY UT WOS:000275087600055 PM 20131793 ER PT J AU Andrade, NA McConnell, LL Torrents, A Ramirez, M AF Andrade, Natasha A. McConnell, Laura L. Torrents, Alba Ramirez, Mark TI Persistence of Polybrominated Diphenyl Ethers in Agricultural Soils after Biosolids Applications SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Polybrominated diphenyl ethers; biosolids; soil ID SEWAGE-SLUDGE; FLAME RETARDANTS; PBDES; FATE; ENVIRONMENT; CONGENERS; LAND; POLLUTANTS; BIPHENYLS; SEDIMENT AB This study examines polybrominated diphenyl ethers (PBDE) levels, trends in biosolids from a wastewater treatment plant, and evaluates potential factors governing PBDE concentrations and the fate in agricultural soils fertilized by biosolids. The mean concentration of the most abundant PBDE congeners in biosolids (Sigma BDE-47, BDE-99, and BDE-209) generated by one wastewater treatment plant was 1250 134 mu g/kg d.w. with no significant change in concentration over 32 months (n = 15). In surface soil samples from the Mid-Atlantic region, average PBDE concentrations in soil from fields receiving no biosolids (5.01 +/- 3.01 mu g/kg d.w.) were 3 times lower than fields receiving one application (.15.2 +/- 10.2 mu g/kg d.w.) and 10 times lower than fields that had received multiple applications (.53.0 +/- 41.7 mu g/kg d.w.), The cumulative biosolids application rate and soil organic carbon were correlated with concentrations and persistence of PBDEs in soil. A model to predict PBDE concentrations in soil after single or multiple biosolids applications provides estimates which fall within a factor of 2 of observed values. C1 [Andrade, Natasha A.; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [McConnell, Laura L.] ARS, USDA, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [Ramirez, Mark] Dist Columbia Water & Sewer Author, Washington, DC 20002 USA. RP Torrents, A (reprint author), Univ Maryland, Dept Civil & Environm Engn, 1153 Martin Hall, College Pk, MD 20742 USA. EM alba@eng.umd.edu FU District of Columbia Water and Sewer Authority FX The authors acknowledge the District of Columbia Water and Sewer Authority for partial funding of this research. NR 32 TC 33 Z9 36 U1 1 U2 33 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 10 PY 2010 VL 58 IS 5 BP 3077 EP 3084 DI 10.1021/jf9034496 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 562WY UT WOS:000275087600069 PM 20151647 ER PT J AU Mullen, LB Woods, HA Schwartz, MK Sepulveda, AJ Lowe, WH AF Mullen, Lindy B. Woods, H. Arthur Schwartz, Michael K. Sepulveda, Adam J. Lowe, Winsor H. TI Scale-dependent genetic structure of the Idaho giant salamander (Dicamptodon aterrimus) in stream networks SO MOLECULAR ECOLOGY LA English DT Article DE death valley model; Dicamptodon aterrimus; genetic structure; scale dependence; stream hierarchy model ID POPULATION-STRUCTURE; LANDSCAPE GENETICS; CONSERVATION GENETICS; MULTILOCUS GENOTYPES; TAILED FROGS; DISPERSAL; ECOLOGY; FLOW; DISTANCE; COLUMBIA AB The network architecture of streams and rivers constrains evolutionary, demographic and ecological processes of freshwater organisms. This consistent architecture also makes stream networks useful for testing general models of population genetic structure and the scaling of gene flow. We examined genetic structure and gene flow in the facultatively paedomorphic Idaho giant salamander, Dicamptodon aterrimus, in stream networks of Idaho and Montana, USA. We used microsatellite data to test population structure models by (i) examining hierarchical partitioning of genetic variation in stream networks; and (ii) testing for genetic isolation by distance along stream corridors vs. overland pathways. Replicated sampling of streams within catchments within three river basins revealed that hierarchical scale had strong effects on genetic structure and gene flow. amova identified significant structure at all hierarchical scales (among streams, among catchments, among basins), but divergence among catchments had the greatest structural influence. Isolation by distance was detected within catchments, and in-stream distance was a strong predictor of genetic divergence. Patterns of genetic divergence suggest that differentiation among streams within catchments was driven by limited migration, consistent with a stream hierarchy model of population structure. However, there was no evidence of migration among catchments within basins, or among basins, indicating that gene flow only counters the effects of genetic drift at smaller scales (within rather than among catchments). These results show the strong influence of stream networks on population structure and genetic divergence of a salamander, with contrasting effects at different hierarchical scales. C1 [Mullen, Lindy B.; Woods, H. Arthur; Sepulveda, Adam J.; Lowe, Winsor H.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Lowe, WH (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM winsor.lowe@umontana.edu RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU Society for Northwestern Vertebrate Biology; Charlotte Martin Foundation; Pacific Rivers Council; Montana River Center FX We thank the following people whose comments improved this manuscript: Fred Allendorf, Stephen Amish, Blake Hossack, Mike Machura, Roger Thorpe, Kim Miller and one anonymous reviewer. Marcie Mullen, Kevin Doyle, Alicia Ward and Blake Hossack helped collect samples. Robb Leary and Fred Allendorf graciously allowed us to use their lab space and equipment in the Montana Conservation Genetics Lab. Sally Painter, Angela Lodmell and Stephen Amish provided assistance genotyping. Claudine Tobalske and Ute Langer provided guidance with GIS analysis.This research was funded by grants from the Society for Northwestern Vertebrate Biology, Charlotte Martin Foundation, the Pacific Rivers Council and the Montana River Center. NR 63 TC 26 Z9 26 U1 0 U2 28 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD MAR 10 PY 2010 VL 19 IS 5 BP 898 EP 909 DI 10.1111/j.1365-294X.2010.04541.x PG 12 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 555XM UT WOS:000274550100007 PM 20149085 ER PT J AU Silva, CJ Onisko, BC Dynin, I Erickson, ML Vensel, WH Requena, JR Antaki, EM Carter, JM AF Silva, Christopher J. Onisko, Bruce C. Dynin, Irina Erickson, Melissa L. Vensel, William H. Requena, Jesus R. Antaki, Elizabeth M. Carter, John Mark TI Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters SO BIOCHEMISTRY LA English DT Article ID TRANSMISSIBLE MINK ENCEPHALOPATHY; ELECTROSPRAY MASS-SPECTROMETRY; SCRAPIE AGENT; SPONGIFORM ENCEPHALOPATHIES; SULFOXIDE REDUCTASES; PROTEIN; OXIDATION; PEPTIDES; IDENTIFICATION; CONVERSION AB Prions are Infectious proteins that are able to recruit a normal Cellular prion protein and convert it into a prion. The mechanism of this conversion IS Unknown. Detailed analysis of the normal cellular prion protein and a corresponding prion has shown they possess identical post-translational modifications and differ solely in conformation. Recent work has suggested that the oxidized form of the methionine at position 2 13 (Met213) plays a role in the conversion of the normal cellular prion protein to the prion conformation and is a prion-specific covalent signature. We developed a sensitive method of quantitating the methionine sulfoxide present at position 2 13 (MetSO213) and used this method to measure file changes In MetSO213 over the time Course of an intracranial challenge, using the 263K strain of hamster-adapted scrapie. These results indicate that the proportion of Met213 that is oxidized decreases over the Course of the disease. We examined the quantity of MetSO213 in PrP(C) and compared it to the amount found in animals terminally afflicted with the 263K, 139H, and drowsy strains of hamster-adapted scrapie. These strains show only low levels of MetSO213 that is comparable to that of PrP(C). These data suggest that MetSO213 does not appear to be a prion-specific covalent Signature. C1 [Silva, Christopher J.; Dynin, Irina; Erickson, Melissa L.; Vensel, William H.; Antaki, Elizabeth M.; Carter, John Mark] Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Onisko, Bruce C.] OniPro Biosci, Kensington, CA 94707 USA. [Requena, Jesus R.] Univ Santiago, Prion Res Unit, Dept Med, Sch Med, E-15706 Santiago, Spain. RP Silva, CJ (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94706 USA. EM christopher.silva@ars.usda.gov RI Carter, John Mark/K-2485-2015; OI Carter, John Mark/0000-0001-8251-4168; Onisko, Bruce/0000-0003-4657-8257; Vensel, William/0000-0001-9454-2705; Silva, Christopher/0000-0003-4521-6377 FU Spanish Ministry of Science and Education [BFU2006-04588/BMC]; European Union [FOOD-CT-2006-023183] FX This work was supported in part by Grant BFU2006-04588/BMC from the Spanish Ministry of Science and Education and Grant FOOD-CT-2006-023183 from the European Union to J.R.R. NR 53 TC 16 Z9 16 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 9 PY 2010 VL 49 IS 9 BP 1854 EP 1861 DI 10.1021/bi901850n PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 560UK UT WOS:000274928500006 PM 20121218 ER PT J AU Peery, MZ Hall, LA Sellas, A Beissinger, SR Moritz, C Berube, M Raphael, MG Nelson, SK Golightly, RT McFarlane-Tranquilla, L Newman, S Palsboll, PJ AF Peery, M. Zachariah Hall, Laurie A. Sellas, Anna Beissinger, Steven R. Moritz, Craig Berube, Martine Raphael, Martin G. Nelson, S. Kim Golightly, Richard T. McFarlane-Tranquilla, Laura Newman, Scott Palsboll, Per J. TI Genetic analyses of historic and modern marbled murrelets suggest decoupling of migration and gene flow after habitat fragmentation SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE dispersal; genetic variation; habitat fragmentation; marbled murrelet; old-growth forest; rescue effects ID EFFECTIVE POPULATION-SIZE; PURSUIT-DIVING SEABIRD; SOURCE-SINK DYNAMICS; BRACHYRAMPHUS-MARMORATUS; THREATENED SEABIRD; ENDANGERED SEABIRD; CONSERVATION; EXTINCTION; DECLINE; DIFFERENTIATION AB The dispersal of individuals among fragmented populations is generally thought to prevent genetic and demographic isolation, and ultimately reduce extinction risk. In this study, we show that a century of reduction in coastal old-growth forests, as well as a number of other environmental factors, has probably resulted in the genetic divergence of marbled murrelets (Brachyramphus marmoratus) in central California, despite the fact that 7 per cent of modern-sampled murrelets in this population were classified as migrants using genetic assignment tests. Genetic differentiation appears to persist because individuals dispersing from northern populations contributed relatively few young to the central California population, as indicated by the fact that migrants were much less likely to be members of parent-offspring pairs than residents (10.5% versus 45.4%). Moreover, a recent 1.4 per cent annual increase in the proportion of migrants in central California, without appreciable reproduction, may have masked an underlying decline in the resident population without resulting in demographic rescue. Our results emphasize the need to understand the behaviour of migrants and the extent to which they contribute offspring in order to determine whether dispersal results in gene flow and prevents declines in resident populations. C1 [Peery, M. Zachariah] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Peery, M. Zachariah; Sellas, Anna; Beissinger, Steven R.; Moritz, Craig] Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. [Hall, Laurie A.] Moss Landing Marine Labs, Moss Landing, CA 95039 USA. [Hall, Laurie A.; Beissinger, Steven R.; Berube, Martine; Palsboll, Per J.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Berube, Martine; Palsboll, Per J.] Stockholm Univ, Dept Genet Microbiol & Toxicol, S-10691 Stockholm, Sweden. [Raphael, Martin G.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia, WA 98512 USA. [Nelson, S. Kim] Oregon State Univ, Dept Fisheries & Wildlife, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. [Golightly, Richard T.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [McFarlane-Tranquilla, Laura] Simon Fraser Univ, Ctr Wildlife Ecol, Burnaby, BC V5A 1S6, Canada. [Newman, Scott] Food & Agr Org United Nations, Infect Dis Grp, I-00153 Rome, Italy. RP Peery, MZ (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr Madison, Madison, WI 53706 USA. EM mpeery@wisc.edu RI Moritz, Craig/A-7755-2012; Beissinger, Steven/F-3809-2012; Palsboll, Per /G-6988-2011 OI Palsboll, Per /0000-0002-4198-7599 FU Humboldt Redwood Company FX Support was provided by the Humboldt Redwood Company. Samples were provided by the Burke Museum, California Academy of Sciences, Museum of Vertebrate Zoology, American Natural History Museum, San Diego Natural History Museum, Donald R. Dickey Collection, Museum of Comparative Zoology and Humboldt State University. Beth Galleher provided GIS support. Robin Waples, Vicki Friesen and two anonymous reviewers provided comments that improved earlier drafts of this paper. Lydia Smith, Jim Harvey and Jon Geller provided laboratory space and technical assistance. NR 58 TC 17 Z9 18 U1 1 U2 45 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD MAR 7 PY 2010 VL 277 IS 1682 BP 697 EP 706 DI 10.1098/rspb.2009.1666 PG 10 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA 547JG UT WOS:000273882800006 PM 19906669 ER PT J AU Del Grosso, SJ Ogle, SM Parton, WJ Breidt, FJ AF Del Grosso, S. J. Ogle, S. M. Parton, W. J. Breidt, F. J. TI Estimating uncertainty in N2O emissions from US cropland soils SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article ID NITROUS-OXIDE EMISSIONS; DAILY SOLAR-RADIATION; UNITED-STATES; AGRICULTURAL LANDS; IPCC METHODOLOGY; COMPLEX TERRAIN; MODEL; DAYCENT; DENITRIFICATION; NITRIFICATION AB A Monte Carlo analysis was combined with an empirically based approach to quantify uncertainties in soil nitrous oxide (N2O) emissions from U.S. croplands estimated with the DAYCENT simulation model. Only a subset of croplands was simulated in the Monte Carlo analysis, which was used to infer uncertainties across the larger spatiotemporal domain. Specifically, one simulation representing dominant weather, soil type, and N inputs was performed for each major commodity crop in the 3000 counties occurring within the conterminous United States. We randomly selected 300 counties for the Monte Carlo analysis and randomly drew model inputs from probability distribution functions (100 iterations). A structural uncertainty estimator was developed by deriving a statistical equation from a comparison of DAYCENT-simulated N2O emissions with measured emissions from experiments in North America. We estimated soil N2O emission of 201 Gg N from major commodity crops in 2007, with a 95% confidence interval (CI) of 133-304 Gg N. This implies a relative CI of 34% below and 51% above the estimate at the national scale, but the CIs tended to be larger at the regional level, particularly in regions with low emissions. Spatial variability in emissions was driven primarily by differences in N inputs from fertilizer and manure, while temporal variability was driven more by N mineralization rates, which are correlated with weather patterns in DAYCENT. A higher portion of total uncertainty was due to model structure compared to model inputs, suggesting that improvements in model algorithms and parameterization are needed to produce results with higher precision and accuracy. C1 [Del Grosso, S. J.] ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO USA. [Del Grosso, S. J.; Ogle, S. M.; Parton, W. J.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Breidt, F. J.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. RP Del Grosso, SJ (reprint author), ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO USA. EM delgro@nrel.colostate.edu FU Agricultural Research Service; U.S. Environmental Protection Agency [EP-W-08-013] FX We thank C. Keough, C. Maxwell, T. Riley, M. Reyes-Fox, and S. Skiles for programming and technical assistance. This publication is based upon work supported by the Agricultural Research Service under the ARS GRACEnet Project and the U.S. Environmental Protection Agency (agreement EP-W-08-013). NR 47 TC 36 Z9 36 U1 3 U2 41 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD MAR 6 PY 2010 VL 24 AR GB1009 DI 10.1029/2009GB003544 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA 565OL UT WOS:000275303300001 ER PT J AU Ximenes, E Kim, Y Mosier, N Dien, B Ladisch, M AF Ximenes, Eduardo Kim, Youngmi Mosier, Nathan Dien, Bruce Ladisch, Michael TI Inhibition of cellulases by phenols SO ENZYME AND MICROBIAL TECHNOLOGY LA English DT Article DE Cellulose; Cellobiose; Cellulases; beta-Glucosidase; Enzyme inhibition; Cellulase inhibitors; Cellulose hydrolysis; T. Reesei; A. niger; Xylan; Aromatic acids; Tannins; Phenols ID LIQUID HOT-WATER; PRETREATED DISTILLERS GRAINS; BETA-GLUCOSIDASE ACTIVITY; GRIND ETHANOL PLANTS; ENZYMATIC-HYDROLYSIS; CORN FIBER; TRICHODERMA-REESEI; PRESSURE COOKING; POPLAR WOOD; CELLULOSE AB Enzyme hydrolysis of pretreated cellulosic materials slows as the concentration of solid biomass material increases. even though the ratio of enzyme to cellulose is kept constant. This form of inhibition is distinct from substrate and product inhibition, and has been noted for lignocellulosic materials including wood, corn stover, switch grass, and corn wet cake at solids concentrations greater than 10 g/L Identification of enzyme inhibitors and moderation of their effects is of considerable practical importance since favorable ethanol production economics require that at least 200 g/L of cellulosic substrates be used to enable monosaccharide concentrations of 100 g/L, which result in ethanol titers of 50 g/L Below about 45 g/L ethanol, distillation becomes energy inefficient. This work confirms that the phenols: vanillin, syringaldehyde, trans-cinnamic acid, and hydroxybenzoic acid, inhibit cellulose hydrolysis in wet cake by endo- and exo-cellulases, and cellobiose hydrolysis by beta-glucosidase. A ratio of 4 mg of vanillin to I mg protein (0.5 FPU) reduces the rate of cellulose hydrolysis by 50%. beta-Glucosidases from Trichoderma reesei and Aspergillus niger are less susceptible to inhibition and require about 10x and 100x higher concentrations of phenols for the same levels of inhibition. Phenols introduced with pretreated cellulose must be removed to maximize enzyme activity. (C) 2010 Elsevier Inc. All rights reserved. C1 [Ladisch, Michael] Purdue Univ, Dept Agr & Biol Engn, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. [Ladisch, Michael] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA. [Dien, Bruce] ARS, Fermentat Biotechnol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Ladisch, M (reprint author), Purdue Univ, Dept Agr & Biol Engn, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. EM ladisch@purdue.edu RI Ximenes, Eduardo/G-5323-2013; OI Ximenes, Eduardo/0000-0001-9087-0218; Dien, Bruce/0000-0003-3863-6664 FU DOE [DE-AC36-99GO10337, DE-FG02-06ER06-03, GO12O26-174, DE-FG02-06ER64301]; DOE BES [0012846]; USDA IFAFS [00-52104-9663]; Mascoma Corporation FX The authors wish to thank Xingya (Linda) Liu, Rick Hendrickson and Thomas Kreke, for their excellent technical assistance, and Dr. Mira Sedlak and Dr David Hogsett for their internal review of this paper. We thank Big River Resources for providing wet cake, and Genencor for their gift of enzymes. The material in this work was supported by DOE grant #DE-AC36-99GO10337; DOE BES Project 0012846, DOE Grant #DE-FG02-06ER06-03, #GO12O26-174 DOE grant #DE-FG02-06ER64301 and USDA IFAFS contract #00-52104-9663, and Mascoma Corporation. NR 55 TC 171 Z9 182 U1 5 U2 82 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0141-0229 J9 ENZYME MICROB TECH JI Enzyme Microb. Technol. PD MAR 5 PY 2010 VL 46 IS 3-4 BP 170 EP 176 DI 10.1016/j.enzmictec.2009.11.001 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 558UO UT WOS:000274773500003 ER PT J AU Kellndorfer, JM Walker, WS LaPoint, E Kirsch, K Bishop, J Fiske, G AF Kellndorfer, J. M. Walker, W. S. LaPoint, E. Kirsch, K. Bishop, J. Fiske, G. TI Statistical fusion of lidar, InSAR, and optical remote sensing data for forest stand height characterization: A regional-scale method based on LVIS, SRTM, Landsat ETM plus , and ancillary data sets SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID VEGETATION CANOPY HEIGHT; WAVE-FORM LIDAR; TROPICAL FOREST; MAPPING VEGETATION; ELEVATION DATA; WOODY BIOMASS; RADAR; TOPOGRAPHY; SAR; BOREAL AB A method is presented to characterize forest stand heights in a 110,000 km(2) region in the eastern United States surrounding the Chesapeake Bay area, driven by a statistical fusion model solely based on remote sensing data. The predicted map was tested against ground survey data from the Forest Inventory and Analysis (FIA) plot network. Input data to the model were 2003 medium footprint lidar data from the Laser Vegetation Imaging Sensor (LVIS) sensor, interferometric radar data from the 2000 Shuttle Radar Topography Mission (SRTM), 1999-2001 Landsat ETM+ data, and ancillary data sets of land cover and canopy density developed for the 2001 National Land Cover Database. In the presented approach, the interferometric synthetic aperture radar (InSAR), optical, and ancillary data sets were masked to the forested areas of the study region and used to segment the raster data stack. The generated image objects closely represented quasi-homogenous forest stands. For a small region in the study area covered by an LVIS acquisition, LVIS lidar data were then used within the established segments to extract lidar-based mean forest stand heights. Subsequently these LVIS mean stand heights were used as the response variable to the statistical prediction model (randomForest) which had segment-based metrics like mean InSAR height (derived from SRTM minus ground digital elevation model data from the National Elevation Data set), mean optical reflectance ( derived from Landsat ETM+ Tassled Cap Data), and ancillary metrics as predictive variables. The model developed over the area where LVIS data were available was then applied to map the entire study region. Independent validation of the model was performed in two ways. First, splitting of the model data stack into training and independent testing populations, i.e., testing on LVIS data. This test was deemed to describe the model performance within the LVIS swath. Second, predicted heights were compared to plot height metrics derived from FIA data in the entire study region, thus testing the validity of the model across the larger study area. Results, which are somewhat tampered by the time disconnect between the various data collections, showed the validity and usefulness of this approach. Independent LVIS testing resulted in a correlation coefficient r = 0.83 with an RMSE of 3.0 m (9% error), independent FIA data tested with r = 0.71 with an RMSE of 4.4 m (13% error). C1 [Kellndorfer, J. M.; Walker, W. S.; Kirsch, K.; Bishop, J.; Fiske, G.] Woods Hole Res Ctr, Falmouth, MA 02540 USA. [LaPoint, E.] US Forest Serv, Forest Inventory & Anal Program, USDA, Durham, NH 03824 USA. RP Kellndorfer, JM (reprint author), Woods Hole Res Ctr, Falmouth, MA 02540 USA. EM josefk@whrc.org FU NASA [NNG05GI27G] FX Work presented in this paper was funded under the NASA Carbon Cycle Science project "The National Biomass and Carbon Dataset 2000: A High Spatial Resolution Baseline to Reduce Uncertainty in Carbon Accounting and Flux Modeling'' (NASA grant NNG05GI27G). The authors acknowledge the U.S. Department of Agriculture Forest Service Forest Inventory and Analysis (FIA) Program for provision of independent testing data. The NASA LVIS Team is acknowledged for provision of LVIS data used in this research. Colin Homer, Dean Gesch, and their teams at the U.S. Geological Service EROS Data Center in Sioux Falls are acknowledged for their collaboration in obtaining NLCD 2001 and NED data. NR 50 TC 27 Z9 27 U1 5 U2 33 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0148-0227 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD MAR 4 PY 2010 VL 115 AR G00E08 DI 10.1029/2009JG000997 PG 10 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 565UG UT WOS:000275319700001 ER PT J AU Bolon, YT Joseph, B Cannon, SB Graham, MA Diers, BW Farmer, AD May, GD Muehlbauer, GJ Specht, JE Tu, ZJ Weeks, N Xu, WW Shoemaker, RC Vance, CP AF Bolon, Yung-Tsi Joseph, Bindu Cannon, Steven B. Graham, Michelle A. Diers, Brian W. Farmer, Andrew D. May, Gregory D. Muehlbauer, Gary J. Specht, James E. Tu, Zheng Jin Weeks, Nathan Xu, Wayne W. Shoemaker, Randy C. Vance, Carroll P. TI Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean SO BMC PLANT BIOLOGY LA English DT Article ID ACETYL-COA CARBOXYLASE; DIFFERENTIALLY EXPRESSED GENES; FEATURE POLYMORPHISM DISCOVERY; QUANTITATIVE TRAIT LOCUS; OIL CONTENT; PHOSPHOENOLPYRUVATE CARBOXYLASE; TRANSCRIPTOME ANALYSIS; MEDICAGO-TRUNCATULA; MESSENGER-RNA; FATTY-ACID AB Background: The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content. Results: A soybean near-isogenic line (NIL) pair contrasting in seed protein and differing in an introgressed genomic segment containing the LG I protein QTL was used as a resource to demarcate the QTL region and to study variation in transcript abundance in developing seed. The LG I QTL region was delineated to less than 8.4 Mbp of genomic sequence on chromosome 20. Using Affymetrix (R) Soy GeneChip and high-throughput Illumina (R) whole transcriptome sequencing platforms, 13 genes displaying significant seed transcript accumulation differences between NILs were identified that mapped to the 8.4 Mbp LG I protein QTL region. Conclusions: This study identifies gene candidates at the LG I protein QTL for potential involvement in the regulation of protein content in the soybean seed. The results demonstrate the power of complementary approaches to characterize contrasting NILs and provide genome-wide transcriptome insight towards understanding seed biology and the soybean genome. C1 [Bolon, Yung-Tsi; Vance, Carroll P.] ARS, USDA, Plant Res Unit, St Paul, MN 55108 USA. [Joseph, Bindu] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Cannon, Steven B.; Graham, Michelle A.; Weeks, Nathan; Shoemaker, Randy C.] ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Farmer, Andrew D.; May, Gregory D.] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Muehlbauer, Gary J.; Vance, Carroll P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Specht, James E.] Univ Nebraska, Dept Agron, Lincoln, NE 68583 USA. [Tu, Zheng Jin; Xu, Wayne W.] Univ Minnesota, Minnesota Supercomp Inst, Minneapolis, MN 55455 USA. RP Vance, CP (reprint author), ARS, USDA, Plant Res Unit, St Paul, MN 55108 USA. EM vance004@umn.edu FU U.S. Department of Agriculture; Agricultural Research Service; Current Research Information System (CRIS) [3640-21000-024-00D]; Minnesota Soybean Research Promotion Council; United Soybean Board FX This work was supported by the U.S. Department of Agriculture, Agricultural Research Service, Current Research Information System (CRIS No. 3640-21000-024-00D). We are grateful for funding from the Minnesota Soybean Research and Promotion Council and for funding from the United Soybean Board. NR 116 TC 34 Z9 36 U1 2 U2 16 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAR 3 PY 2010 VL 10 AR 41 DI 10.1186/1471-2229-10-41 PG 24 WC Plant Sciences SC Plant Sciences GA 573CW UT WOS:000275887000001 PM 20199683 ER PT J AU Pittas, AG Chung, M Trikalinos, T Mitri, J Brendel, M Patel, K Lichtenstein, AH Lau, J Balk, EM AF Pittas, Anastassios G. Chung, Mei Trikalinos, Thomas Mitri, Joanna Brendel, Michael Patel, Kamal Lichtenstein, Alice H. Lau, Joseph Balk, Ethan M. TI Systematic Review: Vitamin D and Cardiometabolic Outcomes SO ANNALS OF INTERNAL MEDICINE LA English DT Review ID PLACEBO-CONTROLLED TRIAL; D SUPPLEMENTATION; BLOOD-PRESSURE; CARDIOVASCULAR-DISEASE; DOUBLE-BLIND; 25-HYDROXYVITAMIN-D LEVELS; CALCIUM SUPPLEMENTATION; MYOCARDIAL-INFARCTION; INCIDENT HYPERTENSION; POSTMENOPAUSAL WOMEN AB Background: Vitamin D may modify risk for cardiometabolic outcomes (type 2 diabetes, hypertension, or cardiovascular disease). Purpose: To examine the association between vitamin D status, including the effect of vitamin D supplementation, and cardiometabolic outcomes in generally healthy adults. Data Sources: English-language studies in MEDLINE (inception to 4 November 2009) and the Cochrane Central Register of Controlled Trials (fourth quarter of 2009). Study Selection: 11 reviewers screened citations to identify longitudinal cohort studies that reported associations between vitamin D status and cardiometabolic outcomes, including randomized trials of vitamin D supplementation. Data Extraction: 5 independent reviewers extracted data about study conduct, participant characteristics, outcomes, and quality. Differences were resolved by consensus. Data Synthesis: 13 observational studies (14 cohorts) and 18 trials were eligible. Three of 6 analyses (from 4 different cohorts) reported a lower incident diabetes risk in the highest versus the lowest vitamin D status groups. Eight trials found no effect of vitamin D supplementation on glycemia or incident diabetes. In meta-analysis of 3 cohorts, lower 25-hydroxyvitamin D concentration was associated with incident hypertension (relative risk, 1.8 [95% CI, 1.3 to 2.4]). In meta-analyses of 10 trials, supplementation nonsignificantly reduced systolic blood pressure (weighted mean difference, -1.9 mm Hg [CI, -4.2 to 0.4 mm Hg]) and did not affect diastolic blood pressure (weighted mean difference, -0.1 mm Hg [CI, -0.7 to 0.5 mm Hg]). Lower 25-hydroxyvitamin D concentration was associated with incident cardiovascular disease in 5 of 7 analyses (6 cohorts). Four trials found no effect of supplementation on cardiovascular outcomes. Limitations: Studies included primarily white participants. Observational studies were heterogeneous. Several trials reported post hoc analyses. Conclusion: The association between vitamin D status and cardiometabolic outcomes is uncertain. Trials showed no clinically significant effect of vitamin D supplementation at the dosages given. Primary Funding Source: National Institute of Diabetes and Digestive and Kidney Disease, the National Institutes of Health Office of Dietary Supplements, U. S. Food and Drug Administration, Agency for Healthcare Research and Quality, and Public Health Agency of Canada. C1 [Balk, Ethan M.] Tufts Univ, Tufts Evidence Based Practice Ctr, Tufts Med Ctr, Boston, MA 02111 USA. Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Balk, EM (reprint author), Tufts Univ, Tufts Evidence Based Practice Ctr, Tufts Med Ctr, Box 63,800 Washington St, Boston, MA 02111 USA. EM ebalk@tuftsmedicalcenter.org FU AHRQ [HHSA 290-2007-10055-I]; National Institute of Diabetes and Digestive and Kidney Disease [R01DK76092, R01DK79003, R21DK78867]; U.S. Department of Health and Human Services; Public Health Agency of Canada FX By AHRQ (contract HHSA 290-2007-10055-I), National Institutes of Health (research grants R01DK76092 and R01DK79003 from the National Institute of Diabetes and Digestive and Kidney Disease and the Office of Dietary Supplements and grant R21DK78867 from the National Institute of Diabetes and Digestive and Kidney Disease), U.S. Department of Health and Human Services, and the Public Health Agency of Canada. NR 60 TC 375 Z9 389 U1 4 U2 40 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 EI 1539-3704 J9 ANN INTERN MED JI Ann. Intern. Med. PD MAR 2 PY 2010 VL 152 IS 5 BP 307 EP W101 DI 10.7326/0003-4819-152-5-201003020-00009 PG 14 WC Medicine, General & Internal SC General & Internal Medicine GA 565XJ UT WOS:000275329600006 PM 20194237 ER PT J AU Guallar, E Miller, ER Ordovas, JM Stranges, S AF Guallar, Eliseo Miller, Edgar R., III Ordovas, Jose M. Stranges, Saverio TI Vitamin D Supplementation in the Age of Lost Innocence SO ANNALS OF INTERNAL MEDICINE LA English DT Editorial Material ID RANDOMIZED CONTROLLED-TRIALS; METAANALYSIS; PREVENTION; MORTALITY; HEALTH; RISK; FRACTURES; CANCERS C1 [Guallar, Eliseo; Miller, Edgar R., III] Johns Hopkins Bloomberg Sch Publ Hlth, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD 21205 USA. [Guallar, Eliseo; Miller, Edgar R., III; Ordovas, Jose M.] Natl Ctr Cardiovasc Res CNIC, Madrid 28029, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Stranges, Saverio] Univ Warwick, Sch Med, Hlth Sci Res Inst, Coventry CV4 7AL, W Midlands, England. RP Guallar, E (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, Welch Ctr Prevent Epidemiol & Clin Res, 2024 E Monument St,Room 2-639, Baltimore, MD 21205 USA. EM eguallar@jhsph.edu RI STRANGES, SAVERIO/F-3273-2010; Guallar, Eliseo/D-3807-2014; OI Guallar, Eliseo/0000-0002-4471-9565; Ordovas, Jose/0000-0002-7581-5680 NR 24 TC 10 Z9 10 U1 0 U2 2 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 EI 1539-3704 J9 ANN INTERN MED JI Ann. Intern. Med. PD MAR 2 PY 2010 VL 152 IS 5 BP 327 EP W106 DI 10.7326/0003-4819-152-5-201003020-00013 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA 565XJ UT WOS:000275329600009 PM 20194240 ER PT J AU Hatle, J Oppert, B AF Hatle, J. Oppert, B. TI Insect gut physiology and nutrition: Insights into diverse systems SO AFRICAN ENTOMOLOGY LA English DT Review DE beetle transcriptome; springtail electrophysiology; arboviruses; mosquito plant feeding; bee diets; Fourth International Conference in Africa for Comparative Physiology and Biochemistry ID SCHISTOCERCA-GREGARIA FORSKAL; RED FLOUR BEETLE; TRIBOLIUM-CASTANEUM; LIFE-SPAN; ANOPHELES-GAMBIAE; AEDES-ALBOPICTUS; DESERT LOCUST; TRANSCRIPTOME; GRASSHOPPERS; RESTRICTION AB Diverse systems with distinctive approaches characterized the presentations in the Insect Gut Physiology and Nutrition Symposium, held during the Fourth International Conference in Africa for Comparative Physiology and Biochemistry at Mara, Kenya, in July 2008. Within the broad topic of insect nutrition, symposium talks examined the feeding behaviour and plant preference of mosquitoes, the effects of dietary levels of protein on grasshopper reproduction and longevity, and the dependence of honeybee survival on certain dietary components. Gut physiology was represented by a sophisticated study of membrane potential in the springtail, the gut as a point of infection for Sindbis virus in mosquito, and a study of the gut transcriptome and proteome of a coleopteran storage pest. A study of chemical ecology described methods currently being used in Kenya to control locust damage to crops. These presentations provided unique perspectives of insect nutrition and gut physiology as related to insect control or improved understanding of beneficial insects. C1 [Hatle, J.] Univ N Florida, Dept Biol, Jacksonville, FL 32224 USA. [Oppert, B.] ARS, USDA, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. RP Hatle, J (reprint author), Univ N Florida, Dept Biol, 1 UNF Dr, Jacksonville, FL 32224 USA. EM jhatle@unf.edu NR 28 TC 1 Z9 1 U1 2 U2 27 PU ENTOMOLOGICAL SOC SOUTHERN AFRICA PI HATFIELD PA PO BOX 13162, HATFIELD 0028, SOUTH AFRICA SN 1021-3589 J9 AFR ENTOMOL JI Afr. Entomol. PD MAR PY 2010 VL 18 IS 1 BP 1 EP 7 DI 10.4001/003.018.0101 PG 7 WC Entomology SC Entomology GA 616EI UT WOS:000279191200001 ER PT J AU Mockrin, MH AF Mockrin, Miranda H. TI Duiker demography and dispersal under hunting in Northern Congo SO AFRICAN JOURNAL OF ECOLOGY LA English DT Article DE Central Africa; Cephalophus; Congo; duiker; hunting; source-sink ID RAIN-FOREST MAMMALS; AFRICAN FOREST; EXPLOITATION; MANAGEMENT; DYNAMICS; BEHAVIOR; BUSHMEAT; SUBJECT; IMPACTS; ECOLOGY AB Duikers are the most heavily hunted species across forested Central and West Africa. Although these species form a vital food resource for people, little is known about their ecology and demography. The information available to date was collected from populations protected from hunting. However, hunting can profoundly alter demography and behaviour, such as dispersal, which in turn determine the sustainability of harvest. To address this research gap, I used radiotelemetry to examine duiker demography under hunting in Congo-Brazzaville. Among blue duikers (Philantoba monticola, n = 17) I found annual survival rates (0.94), immature dispersal rates (0.25), and average home ranges (5.5 ha) that largely coincided with previous research from unhunted regions. Neighbouring animals all showed some home range overlap, in contrast to previous studies which found blue duikers to be strictly territorial. Although limited by sample size, immature animals' dispersal distances were relatively high (1.5 km, n = 2). Animals' dispersal rates do not appear to be greatly increased by hunting pressure, although source-sink theory depends upon high rates of dispersal to maintain the elevated and localized hunting offtakes observed around settlements. Building a fuller understanding of duiker demography under hunting, including dispersal, will be essential for conservation and management efforts. C1 [Mockrin, Miranda H.] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY 10025 USA. RP Mockrin, MH (reprint author), US Forest Serv, USDA, 1400 Independence Ave SW MC1113, Washington, DC 20250 USA. EM mmockrin@gmail.com RI Piper, Walter/B-7908-2009 FU WCS; National Science Foundation; Columbia University; Center for Environmental Research and Conservation at Columbia University; American Society of Mammalogists; Roger Williams Zoo FX WCS-Congo provided generous logistic support and assistance. The Congolese government and CIB logging company provided research access. K. H. Redford, R. F. Rockwell, M. Cords, R. Schroeder, and D. Wilkie contributed valuable research guidance. WCS Field Vet Program assisted with animal captures. Financial support for this research was provided by WCS, a National Science Foundation Graduate Research Fellowship, Columbia University Dissertation Traveling Fellowship, Center for Environmental Research and Conservation at Columbia University, American Society of Mammalogists, and the Roger Williams Zoo. NR 38 TC 6 Z9 6 U1 1 U2 21 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0141-6707 J9 AFR J ECOL JI Afr. J. Ecol. PD MAR PY 2010 VL 48 IS 1 BP 239 EP 247 DI 10.1111/j.1365-2028.2009.01107.x PG 9 WC Ecology SC Environmental Sciences & Ecology GA 551IF UT WOS:000274199800030 ER PT J AU Troen, AM French, EE Roberts, JF Selhub, J Ordovas, JM Parnell, LD Lai, CQ AF Troen, Aron M. French, Emily E. Roberts, Jessica F. Selhub, Jacob Ordovas, Jose M. Parnell, Laurence D. Lai, Chao-Qiang TI Lifespan modification by glucose and methionine in Drosophila melanogaster fed a chemically defined diet (vol 29, pg 29, 2007) SO AGE LA English DT Correction C1 [Troen, Aron M.; French, Emily E.; Roberts, Jessica F.; Selhub, Jacob; Ordovas, Jose M.; Parnell, Laurence D.; Lai, Chao-Qiang] Tufts Univ, Vitamin Metab & Aging Lab, Jean Mayer USDA Human Nutr Res Ctr, Boston, MA 02111 USA. RP Troen, AM (reprint author), Tufts Univ, Vitamin Metab & Aging Lab, Jean Mayer USDA Human Nutr Res Ctr, 711 Washington St, Boston, MA 02111 USA. EM aron.troen@tufts.edu NR 4 TC 2 Z9 2 U1 0 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0161-9152 J9 AGE JI Age PD MAR PY 2010 VL 32 IS 1 BP 123 EP 123 DI 10.1007/s11357-010-9133-0 PG 1 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA 561GD UT WOS:000274963000012 ER PT J AU Glaz, B Morris, DR AF Glaz, Barry Morris, Dolen R. TI Sugarcane Responses to Water-Table Depth and Periodic Flood SO AGRONOMY JOURNAL LA English DT Article ID EVERGLADES AGRICULTURAL AREA; GAS-TRANSPORT; REGISTRATION; HYPOXIA AB Sugarcane (Saccharum spp.) is exposed to periodic floods and shallow water tables in Florida due to practices that reduce P discharge to the Everglades. This study examined the yields and juice quality of four sugarcane cultivars (CP 88-1762, Cl 89-2143, CP 89-2376, and CP 96-1252) maintained at constant water-table depths near 20 (20CWT) and 45 cm (45CWT) and with periodic summer flooding. Prescribed lysimeters were flooded for the first 7 d of five, five, and four 21-d cycles in 2005 (plant-cane crop), 2006 (first-ratoon crop), and 2007 (second-ratoon crop), respectively. These treatments generally remained flooded during the next 7 d while they received no irrigation or drainage, and were drained to 20 (20FWT) or 45 cm (45FWT) for the final 7 d of each cycle. Water treatment affected CRS only in the plant-cane crop, where 45CWT had lower CRS than 20CWT, 20FWT, and 45FWT. Yields of cane and sucrose of CP 89-2143 were least affected by water treatments. Reductions in cane and sucrose yields at a 20 compared with a 45 cm water-table depth were common for the other three cultivars, but yields under the 20CWT vs. 20FWT or 45CWT vs. 45FWT treatments were generally similar. These results suggest that sugarcane roots function well in flood for up to 14 d, but do not grow well into saturated soil. This provides new options for sustaining high yields of sugarcane exposed to shallow water tables and floods; verification of root responses could enhance strategies to sustain yields while reducing P discharge. C1 [Glaz, Barry; Morris, Dolen R.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Glaz, B (reprint author), USDA ARS, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. NR 31 TC 6 Z9 6 U1 1 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 372 EP 380 DI 10.2134/agronj2009.0262 PG 9 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300002 ER PT J AU Singer, JW Chase, CA Kohler, KA AF Singer, Jeremy W. Chase, Craig A. Kohler, Keith A. TI Profitability of Cropping Systems Featuring Tillage and Compost SO AGRONOMY JOURNAL LA English DT Article ID CORN; YIELD; GROWTH; PERFORMANCE; ECONOMICS; ROTATIONS AB Productivity rather than profitability is often used to compare agronomic systems. The objective of this study was to compare profitability of moldboard plow, chisel plow, and no-tillage with or without composted animal manure in a corn (Zea mays L.)-soybean [Glycine max (L.) Merr.]-wheat (Triticum aestivum L.)/clover (Trifolium spp.) rotation during three rotation cycles. Corn and soybean grain and seed yield exhibited a tillage x compost amendment interaction. Yield in moldboard and chisel plow with or without compost was similar, but yield in no-tillage with compost was 8 and 5% greater than without compost for corn and soybean. Wheat yielded 5% higher in moldboard and chisel plow than no-tillage and 4% higher in compost than no-compost amendment. Wheat returns were similar among tillage and 7% higher when compost was amended. Corn production with or without compost amendment had similar returns in moldboard plow. Corn in chisel plow with compost had 8% greater returns than the no-compost treatment. Corn in no-tillage with compost had 15% greater returns with compost amendment than without. Similar corn returns were generated for all tillage systems if compost was applied. Soybean production using no-tillage had 9% greater returns than without compost and greater returns than moldboard and chisel plow with or without compost. Summing returns across the three-crop rotation indicated cycling nutrients through compost application exhibits a functional synergy in no-tillage and chisel plow but not moldboard plow for these crops, which enhances their profitability. C1 [Singer, Jeremy W.] USDA ARS, Ames, IA 50011 USA. [Chase, Craig A.] Iowa State Univ, Tripoli, IA 50696 USA. RP Singer, JW (reprint author), USDA ARS, Ames, IA 50011 USA. RI Singer, Jeremy/G-6260-2010 NR 15 TC 3 Z9 3 U1 2 U2 5 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 450 EP 456 DI 10.2134/agronj2009.0327 PG 7 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300009 ER PT J AU Williams, MM AF Williams, Martin M., II TI Biological Significance of Low Weed Population Densities on Sweet Corn SO AGRONOMY JOURNAL LA English DT Article ID PLANTING DATE; GIANT RAGWEED; INTERFERENCE; MAIZE; COMPETITION; GROWTH; EAR AB Some weed plants escape current weed management systems in nearly all sweet corn (Zea mays L.) fields. Decisions to target escaped weeds, and justify the added expense, require knowledge of the biological significance of low weed population densities on the crop. The objectives were to (i) quantify giant ragweed (Ambrosia trifida L.) area of influence (AOI) on sweet corn, and (ii) investigate potential links among giant ragweed AOI and crop growth, development, and yield attributes. All measured crop attributes were influenced by giant ragweed AOI, including plant height near silk emergence (HT), thermal time to silk emergence, green ear mass (GMASS), husked car mass (HMASS), filled ear length (LENGTH), ear width at midpoint (WIDTH), and kernel moisture (MOIST). Proportion of silked plants declined for sweet corn within 160 cm of giant ragweed, with less than one-half of the crop plants producing a marketable car within 42 cm of giant ragweed. Weed interference harmed ear attributes most when crop development was delayed, as evidenced by path analysis of giant ragweed's direct and indirect associations with yield attributes. Even the lowest population density of giant ragweed can be costly, with yield loss estimates ranging from $0.86 to $8.75 per weed plant, depending on crop marker type. C1 Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, 1102 S Goodwin Ave, Urbana, IL 61801 USA. NR 18 TC 7 Z9 7 U1 0 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 464 EP 468 DI 10.2134/agronj2009.0308 PG 5 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300011 ER PT J AU Helms, TC Scott, RA Schapaugh, WT Goos, RJ Franzen, DW Schlegel, AJ AF Helms, T. C. Scott, R. A. Schapaugh, W. T. Goos, R. J. Franzen, D. W. Schlegel, A. J. TI Soybean Iron-Deficiency Chlorosis Tolerance and Yield Decrease on Calcareous Soils SO AGRONOMY JOURNAL LA English DT Article ID GENOTYPES AB Cultivar selection is one of the best ways to manage iron-deficiency chlorosis (IDC) problems in soybean [Glycine max (L.) Merr.]. The objective was to determine if precision farming techniques of planting IDC-tolerant cultivars in calcareous soil areas and high-yielding cultivars in non-IDC areas would increase soybean yield. We used paired sites within the same field. The sites were located in areas of a field where IDC was present and absent. The same commercial soybean cultivars were planted on the paired sites. Results showed that visual scores for IDC could not identify the highest-yielding cultivar in IDC-affected areas. If the only information available to growers is yield on non-IDC sites and visual I DC ratings, then the yield of the whole field could be increased by planting two different cultivars. If yield data from replicated performance testing of numerous different cultiars was available for I DC sites and also for non-IDC sites, then growers may be able to identify a single cultivar that has high yield across the entire field. C1 [Helms, T. C.] NDSU Dep 7670, Dept Plant Sci, Fargo, ND 58108 USA. [Goos, R. J.; Franzen, D. W.] NDSU Dep 7680, Dept Soil Sci, Fargo, ND 58108 USA. [Scott, R. A.] USDA ARS, Beltsville, MD 20705 USA. [Schapaugh, W. T.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Schlegel, A. J.] Kansas State Univ, SW Res Extens Ctr, Tribune, KS 67879 USA. RP Helms, TC (reprint author), NDSU Dep 7670, Dept Plant Sci, POB 6050, Fargo, ND 58108 USA. FU North Central Soybean Program FX This research was supported in part by grants from the North Central Soybean Program Dr Lori Scott initiated the grant and coordinated the first year of the research. NR 11 TC 8 Z9 9 U1 1 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 492 EP 498 DI 10.2134/agronj2009.0317 PG 7 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300015 ER PT J AU Balkcom, KS Arriaga, FJ Balkcom, KB Boykin, DL AF Balkcom, Kipling S. Arriaga, Francisco J. Balkcom, Kris B. Boykin, Deborah L. TI Single- and Twin-Row Peanut Production within Narrow and Wide Strip Tillage Systems SO AGRONOMY JOURNAL LA English DT Article ID SPOTTED WILT; WINTER COVER; SEEDING RATE; SOIL; MANAGEMENT; PATTERN; COTTON; QUALITY; PHORATE; CROP AB Increased production costs and potential benefits of maintaining surface residue has renewed Interest in conservation tillage systems for peanut (Arachis hypogaea L.) production. We determined surface residue cover from rye (Secale cereale L.) or oat (Avena saliva L.) cover crops after two strip tillage systems (narrow vs. wide) and planting operations with different row configurations (single vs. twin). We also compared plant populations, yields, and total sound mature kernels for three peanut cultivars ('ANorden', 'AP-3', and 'Georgia-02C') across each treatment combination. Seven site-years were examined across similar soil types in Alabama and northern Florida during the 2004 to 2006 growing seasons. The highest surface residue counts were for the narrow tillage system planted in single rows. Final plant stands were influenced by an interaction between cultivar and row configuration, with 'ANorden' planted in single rows below recommended rates. Peanut yields were affected by strip tillage system and row configuration, but differences among cultivars were also observed. Twin-row peanut yields were 5% greater than single-row peanut yields in the narrow strip tillage system but were similar across strip tillage systems. Cultivars 'AP-3' and 'Georgia-02C' yielded 20% higher than 'ANorden'. Total sound mature kernels were only affected by peanut cultivar, with the cultivar 'Georgia-02C' producing the highest-quality peanut, followed by 'ANorden' and 'AP-3'. These results indicate that growers interested in using twin rows for peanut production can also take advantage of a narrow strip tillage system that maximizes surface residue coverage and subsequent benefits. C1 [Balkcom, Kipling S.; Arriaga, Francisco J.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Balkcom, Kris B.] Wiregrass Res & Extens Ctr, Headland, AL 36345 USA. [Boykin, Deborah L.] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. RP Balkcom, KS (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. NR 27 TC 5 Z9 5 U1 2 U2 3 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 507 EP 512 DI 10.2134/agronj2009.0334 PG 6 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300017 ER PT J AU Nielsen, DC Vigil, MF AF Nielsen, David C. Vigil, Merle F. TI Precipitation Storage Efficiency during Fallow in Wheat-Fallow Systems SO AGRONOMY JOURNAL LA English DT Article ID CENTRAL GREAT-PLAINS; SOIL WATER STORAGE; CROP ROTATIONS; STRAW MULCH; TILLAGE; RESIDUE; SURFACE AB Precipitation storage efficiency (PSE) is the fraction of precipitation received in a given time period that is stored in the soil. Average fallow PSE for Great Plains wheat (Triticum aestivum L.)-fallow (W-F) production systems have ranged widely (10-53%). Study objectives were to compare PSE in conventionally tilled (CT) and no-till (NT) W-F systems over 10 seasons at Akron, CO, against published values and to identify meteorological conditions that may influence PSE. Soil water measurements were made four times during each fallow period, dividing the fallow season into three periods (first summer, fall winter spring, second summer). Precipitation was measured in the plot area and other meteorological conditions were measured at a nearby weather station. The 14-mo fallow PSE averaged 20% (range 8-34%) for CT and 35% (range 20-51%) for NT, much lower than previously reported for NT at Akron. During the second summer period, PSE was not different between the two systems. The largest PSE difference between the two systems was seen during the fall winter spring period (32 vs. 81%). Fallow soil water increased an average of 111 mm under CT and 188 mm under NT. The PSE during the three fallow periods was related to tillage, precipitation, air temperature, vapor pressure deficit, and wind speed, but sometimes counter-intuitively. A simple linear regression using inputs of tillage system, percentage of fallow precipitation events with amounts between 5 and 15 mm, and percentage of fallow precipitation events with amounts > 25 mm can be used to estimate PSE and fallow period water storage. C1 [Nielsen, David C.; Vigil, Merle F.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. RP Nielsen, DC (reprint author), USDA ARS, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. RI Nielsen, David/A-8044-2009 OI Nielsen, David/0000-0002-8240-7183 NR 20 TC 30 Z9 32 U1 0 U2 17 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 537 EP 543 DI 10.2134/agronj2009.0348 PG 7 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300021 ER PT J AU Gaskin, JW Speir, RA Harris, K Das, KC Lee, RD Morris, LA Fisher, DS AF Gaskin, Julia W. Speir, R. Adam Harris, Keith Das, K. C. Lee, R. Dewey Morris, Lawrence A. Fisher, Dwight S. TI Effect of Peanut Hull and Pine Chip Biochar on Soil Nutrients, Corn Nutrient Status, and Yield SO AGRONOMY JOURNAL LA English DT Article ID BLACK CARBON; CHARCOAL; QUALITY; CROP; AMENDMENTS; FERTILITY; INCREASES; NITROGEN; TILLAGE; TROPICS AB Pyrolysis is the anaerobic thermal conversion of biomass for energy production. It offers an option of returning carbon and nutrients to the soil while producing energy. The Ultisols in the southeastern United States have Inherently low soil organic carbon and fertility, and may benefit from the addition of biochar from pyrolysis. Our objectives were to evaluate the effect of peanut hull and pine chip biochars on soil nutrients, corn (Zen mays L.) nutrient status and yield in a Kandiudult for two growing seasons (2006 and 2007). Experiments for each biochar source were conducted as completely randomized designs with the biochar applied at 0, 11, and 22 Mg ha(-1) with and without N fertilizer. Nitrogen in the peanut hull biochar (209 kg ha(-1) at 11 Mg ha(-1) rate) was not available during the study based on corn tissue concentrations. The peanut hull biochar linearly increased Mehlich I K, Ca, and Mg in the surface sod (0-15 cm). The increased available K was reflected in the plant tissue analysis at corn stage RI in 2006, but not in 2007. Pine chip biochar decreased soil pH, but had no effect on other nutrients except Mehlich I Ca. In the peanut hull biochar experiment, grain yields decreased at the 22 Mg ha(-1) rate in the fertilized treatments. In the pine chip biochar experiment, grain yields decreased linearly with application rate in 2006, but this did not persist in 2007. Overall yield responses to biochar were smaller than expected based on previous studies. C1 [Gaskin, Julia W.; Speir, R. Adam; Harris, Keith; Das, K. C.] Univ Georgia, Biol & Agr Engn Dep, Athens, GA 30602 USA. [Lee, R. Dewey] Univ Georgia, Crop & Soil Sci Dep, Tifton, GA 31793 USA. [Morris, Lawrence A.] Univ Georgia, Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Fisher, Dwight S.] USDA ARS, JPC Nat Resource Conservat Ctr, Watkinsville, GA 30677 USA. RP Gaskin, JW (reprint author), Univ Georgia, Biol & Agr Engn Dep, Athens, GA 30602 USA. FU U S Department of Energy FX We wish to acknowledge the thoughtful review by Harry Schomberg who greatly improved this manuscript We also wish to thank Christoph Steiner and anonymous reviewers for their helpful comments Lee Ogden and Robert Pippen provided valuable field assistance. This project was conducted with funding from the State of Georgia and the U S Department of Energy NR 41 TC 135 Z9 150 U1 15 U2 110 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 623 EP 633 DI 10.2134/agronj2009.0083 PG 11 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300032 ER PT J AU Brink, GE Casler, MD Martin, NP AF Brink, G. E. Casler, M. D. Martin, N. P. TI Meadow Fescue, Tall Fescue, and Orchardgrass Response to Defoliation Management SO AGRONOMY JOURNAL LA English DT Article ID NEUTRAL DETERGENT FIBER; PERENNIAL GRASSES; NUTRITIVE-VALUE; FORAGE; YIELD; PERSISTENCE; CULTIVARS; FREQUENCY; QUALITY; HEIGHT AB Meadow fescue [Schedonorus pratensis (Huds.) P. Beam.] represents an alternative to temperate grasses typically used in forage-livestock systems. Our objective was to compare the productivity, nutritive value, and persistence of diverse meadow fescue cultivars with that of tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] and orchardgrass (Dactyl's glomerata L.) when harvested by regimes representing lax and severe hay production and rotational grazing. 'Azov' (plant introduction strain cross), 'Bartura' (commercial cultivar), and 'Hidden Valley' (naturalized population) meadow fescue, `Barolex' tall fescue, and 'Bronc' orchardgrass were harvested infrequently (40- to 65-d harvest interval) or frequently (when plants reached 25-cm sward height) to a 5- or 10-cm residual sward height (RSH) at two Wisconsin locations in 2 yr. Annual dry matter (DM) yield of all grasses was greater when harvested infrequently (7.46 Mg ha(-1)) than frequently (5.92 Mg ha(-1)), or at 5 cm (7.52 Mg ha(-1)) than 10-cm RSH (5.88 Mg ha(-1)). Tall fescue and orchardgrass annual yield was greater than that of all meadow fescues when harvested infrequently, but differences among grasses were relatively small when harvested frequently, particularly at 10-cm RSH. Neutral detergent fiber digestibility (NDFD) of meadow fescue was 30 to 80 g kg(-1) neutral detergent fiber (NDF) greater than that of tall fescue or orchardgrass at every harvest in all environments. Meadow fescue cultivars were less persistent than tall fescue after 2 yr, but were usually equal to or more persistent than orchardgrass. Meadow fescue should be considered as a viable alternative to tall fescue and orchardgrass in temperate, managed intensive rotational grazing systems due to its comparable yield and superior digestibility. C1 [Brink, G. E.; Casler, M. D.; Martin, N. P.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Brink, GE (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr W, Madison, WI 53706 USA. NR 32 TC 11 Z9 11 U1 0 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 667 EP 674 DI 10.2134/agronj2009.0376 PG 8 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300037 ER PT J AU Cowger, C Weisz, R Anderson, JM Horton, JR AF Cowger, Christina Weisz, Randy Anderson, Joseph M. Horton, J. Ray TI Maize Debris Increases Barley Yellow Dwarf Virus Severity in North Carolina Winter Wheat SO AGRONOMY JOURNAL LA English DT Article ID CEREAL APHIDS; SEASONAL ABUNDANCE; SOUTH-CAROLINA; SMALL GRAINS; HOMOPTERA; IDENTIFICATION; LUTEOVIRUSES; TILLAGE; COLORS; YIELD AB In the eastern United States, wheat (Triticum aestivum L.) is often planted with minimal or no tillage into maize (Zea mays L.) residues. We conducted a field experiment in the North Carolina Piedmont to compare the effects of three maize residue treatments (unchopped, chopped, and removed) on Fusarium head blight (FHB) in two winter wheat cultivars. While FHB levels were too low for meaningful comparisons, severe epidemics of barley/cereal yellow dwarf virus (YDV) did develop in 2 yr out of 3. In those 2 yr, YDV symptoms of discoloration and stunting were greater (P <= 0.001), and yield was lower (P <= 0.01), in plots with maize residue than in plots without maize residue. In the third year, when planting was late because of a severe fall drought, no YDV epidemic developed, and there were no differences in wheat yield due to maize residue treatment (P = 0.25). In the first 2 yr, leaf samples from all plots were assayed for viruses using a multiplexed reverse transcription polymerase chain reaction (RT-PCR) method. The most common YDV serotypes were MAY, PAV, and RPV, which were each detected in at least 46 and 74% of samples in the 2 yr, respectively. Our finding of greater YDV severity in association with surface residue is consistent with the reported aphid preference for high-intensity yellow colors, which we hypothesize attracted aphids preferentially to residue-covered plots in the fall. Our results support a recommendation of seed or seedling Insecticide treatment when planting wheat into heavy unincorporated maize residue in the U.S. Piedmont. C1 [Cowger, Christina] N Carolina State Univ, USDA ARS, Dep Plant Pathol, Raleigh, NC 27695 USA. [Anderson, Joseph M.] Purdue Univ, USDA ARS, Agron Dep, W Lafayette, IN 47907 USA. [Weisz, Randy] N Carolina State Univ, Dep Crop Sci, Raleigh, NC 27695 USA. [Horton, J. Ray] N Carolina Dep Agr & Consumer Serv, Border Belt Tobacco Res Stn, Whiteville, NC 28472 USA. RP Cowger, C (reprint author), N Carolina State Univ, USDA ARS, Dep Plant Pathol, CB7616, Raleigh, NC 27695 USA. NR 39 TC 2 Z9 2 U1 2 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 688 EP 695 DI 10.2134/agronj2009.0357 PG 8 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300039 ER PT J AU Zheljazkov, VD Cantrell, CL Astatkie, T AF Zheljazkov, Valtcho D. Cantrell, Charles L. Astatkie, Tess TI Study on Japanese Cornmint in Mississippi SO AGRONOMY JOURNAL LA English DT Article ID MINT MENTHA-ARVENSIS; ESSENTIAL OILS; PEPPERMINT; NITROGEN; MULCH; PRODUCTIVITY; PIPERASCENS; IRRIGATION; GROWTH; YIELD AB Japanese cornmint (Mentha canadensis L.) is a subtropical essential oil crop grown in Asia and South America. The essential oil of Japanese cornmint is the source for production of crystal (-)-menthol, which is an important aromatic agent used in various industries. The United States is a major importer and consumer of (-)-menthol and de-mentholized oil. Currently there is no production of Japanese cornmint in the United States. A 2-yr study was conducted in Mississippi to evaluate the effect of N application rates (0, 80, and 160 kg ha(-1)) and cut (harvest time, with a first cut in July, and a second cut in October) on herbage yields, essential oil content, and composition of two Japanese cornmint genotypes ('Arvensis 2' and Arvensis 3'). Both cultivars provided two cuts and comparable herbage and oil yields to literature reports. Generally, N application increased fresh herbage and oil yields relative to the untreated control. Herbage, essential oil, (-)-menthol, and (-)-menthone yields were greater from the first cut than from the second for both cultivars. The concentration of(-)-menthol in the oil of both cultivars was approximately 50% in 2007; however, in 2008 the (-)-menthol concentration was 67 to 76% in Arvensis 2' and 73 to 78% in Arvensis 3'. This study demonstrates that Japanese cornmint could be successfully grown in Mississippi and possibly other areas in the southeastern United States with similar environmental conditions. C1 [Zheljazkov, Valtcho D.] N Mississippi Res & Extens Ctr, Verona, MS 38879 USA. [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Astatkie, Tess] Nova Scotia Agr Coll, Dep Engn, Truro, NS B2N 5E3, Canada. RP Zheljazkov, VD (reprint author), N Mississippi Res & Extens Ctr, 5421 Hwy,145 South, Verona, MS 38879 USA. RI Astatkie, Tess/J-7231-2012 OI Astatkie, Tess/0000-0002-9779-8789 FU ARS, Mississippi State University [58-6402-4-026, CRIS MIS-027100] FX This research was funded by ARS Specific Coop Agreement 58-6402-4-026 with CRIS MIS-027100 of Mississippi State University Specific project "Field Establishment of Medicinal Herbs and Potential for Commercial Production" awarded to Dr Jeliazkov (Zbehazkov) The authors thank Vasde Cerven, Thomas Horgan, Marie Rogers, and Amber Reichley for excellent help with experiments and analysis NR 44 TC 6 Z9 6 U1 0 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 696 EP 702 DI 10.2134/agronj2009.0275 PG 7 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300040 ER PT J AU Szogi, AA Bauer, PJ Vanotti, MB AF Szogi, Ariel A. Bauer, Philip J. Vanotti, Matias B. TI Fertilizer Effectiveness of Phosphorus Recovered from Broiler Litter SO AGRONOMY JOURNAL LA English DT Article ID POULTRY LITTER; MANURE; SOIL; SPECIATION; RAINFALL; NITROGEN; COMPOST; RUNOFF; WASTE AB Phosphorus can be recovered from broiler litter using a new process ("quick wash"). However, limited information is available on the beneficial use of this P product as plant fertilizer. The objective of this study was to determine the agronomic effectiveness of this P material. A greenhouse study was conducted with annual ryegrass (Lolium multiflorum Lam.) to compare the recovered phosphorus (RP) to both commercial triple superphosphate (TSP) and raw broiler litter (BL) as a P source. The study consisted of a 7-wk experiment that was repeated three times. Fertilizer rates were 0, 22, 44, 88, and 176 mg P kg(-1) soil. Three harvests of the ryegrass were made at 2-wk intervals. On average, biomass production was higher than the control with no P for plants fertilized with RP, BL, or TSP. Total P uptake increased linearly with application rate for all three fertilizer materials. At the highest application rate, total P uptake was 36.1 mg pot(-1) for TSP, 21.3 mg pot(-1) for the BL, and 17.3 mg pot(-1) for the RP. Chemical analysis of the RP material revealed that more than 90% of the total P in the RP material was mostly as citrate-soluble. Mehlich-3 extractable soil P at the end of the experiment indicated that less RP material dissolved than TSP during this short study. The RP material appears to have potential as a P fertilizer. C1 [Szogi, Ariel A.; Bauer, Philip J.; Vanotti, Matias B.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Szogi, AA (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. FU USDA-ARS [6657-13630-003-00D] FX This research was part of USDA-ARS National Program 206 Manure and By-product Utilization, ARS Project 6657-13630-003-00D "Innovative Animal Manure Treatment Technologies for Enhanced Environmental Quality" The authors arc grateful to Don Watts, Ernie Strickland, Anita Reed, Tabitha Wheeler, and Brian Tyler for greenhouse and laboratory assistance. NR 31 TC 6 Z9 6 U1 0 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 723 EP 727 DI 10.2134/agronj2009.0355 PG 5 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300044 ER PT J AU Sanderson, M AF Sanderson, Matt TI Nutritive Value and Herbage Accumulation Rates of Pastures Sown to Grass, Legume, and Chicory Mixtures SO AGRONOMY JOURNAL LA English DT Article ID CICHORIUM-INTYBUS L.; FORAGE CHICORY; BOTANICAL COMPOSITION; ECOSYSTEM STABILITY; GRAZING MANAGEMENT; PRODUCTIVITY; QUALITY; YIELD; BIODIVERSITY; ORCHARDGRASS AB Planting forage mixtures may benefit pasture herbage production; however, changes in botanical composition could cause variable (unstable) nutritive value. A grazing study was conducted to compare forage mixtures for variation in herbage nutritive value with time and herbage accumulation rate. In August 2001, four mixtures (two, three, six, and nine species of grasses, legumes and chicory, Cichorium intybus L) were established in replicated 1-ha pastures (eight total) in central Pennsylvania. Pastures were grazed by dairy cattle (Bos taurus) from April to September of 2002 and 2003 and sampled frequently for nutritive value and herbage accumulation rate in April, July, and September each year. The intra-annual variation (across all sampling periods within years) in fiber and digestibility was similar among mixtures; however, variation in crude protein (CP) was greater for the six-species mixture than others in 2003. Concentration of CP (range of 155-250 g kg(-1) dry matter) seemed to be controlled by herbage legume proportion (Pearson r of 0.33-0.90), whereas fiber (range of 278-506 g kg(-1)) and digestibility (range of 740-895 g kg(-1)) were controlled by the grass proportion (Pearson r of 0.54-0.88). The three-, six-, and nine-species mixtures (all with chicory) had greater herbage accumulation rates than the orchardgrass (Dactylis glomerata L.)-white clover (Trifolium repens L.) mixture in 2002, a dry year. Accumulation rates were similar among mixtures (avg of 62 kg ha(-1) d(-1) in spring and 47 kg ha(-1) d(-1) in summer) in 2003 with more rainfall. Complex forage mixtures were not inherently unstable in herbage nutritive value. C1 USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Sanderson, M (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. NR 32 TC 9 Z9 9 U1 5 U2 15 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 728 EP 733 DI 10.2134/agronj2009.0374 PG 6 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300045 ER PT J AU de Souza, EG Scharf, PC Sudduth, KA AF de Souza, Eduardo G. Scharf, Peter C. Sudduth, Kenneth A. TI Sun Position and Cloud Effects on Reflectance and Vegetation Indices of Corn SO AGRONOMY JOURNAL LA English DT Article ID LEAF CHLOROPHYLL CONCENTRATION; CANOPY REFLECTANCE; NITROGEN; LEAVES; YIELD; RADIATION; METER; ANGLE AB The reflectance characteristics of plants and plant canopies far from solar noon (i.e., at large solar zenith angles) or with cloudy skies are not well known. This is an obstacle to making real-time, variable-rate N fertilizer applications based on canopy reflectance because such a system must work under cloudy skies and at all times of day. Our objective was to develop spectral radiometer reflectance corrections for variations in incoming sunlight so that the same reflectance reading would be obtained (and the same N recommendation made) for the same plants regardless of time of day or cloud conditions. Passive spectral radiometers were mounted in a stationary position about 25 cm above the corn (Zea mays L.) canopy. Readings were taken from morning until night over several days with a range of sky conditions (sunny, overcast, and partly cloudy). Experiments were done in the field in April and May on greenhouse-grown corn ranging from V10 to R2 growth stages. Sun angle, time of day, and cloud cover all influenced reflectance measured from the corn canopy. When regression models were applied to correct reflectance values to reference conditions for these variables, coefficients of variation were reduced by 29 to 56% for vegetation Indexes and by 43 to 56% for reflectance values. The near-infrared/green ratio and the green normalized difference vegetation index were the indices most sensitive to N deficiency among six analyzed indices. C1 [Scharf, Peter C.; Sudduth, Kenneth A.] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [de Souza, Eduardo G.] Univ Estadual Oeste Parana, Res Team GROSAP, CCET, BR-85814110 Cascavel, PR, Brazil. RP Scharf, PC (reprint author), Univ Missouri, USDA ARS, 214B Waters Hall, Columbia, MO 65211 USA. FU National Council of Scientific and Technological Development-CNN, Brazil; Missouri Precision Agriculture Center FX The authors are grateful for the support provided by the University of Missouri-Columbia and the State University of Western Parana, Unioeste, Brazil They are also grateful for the financial support provided by the National Council of Scientific and Technological Development-CNN, Brazil and the Missouri Precision Agriculture Center The authors thank Randy Miles for his cooperation in using Sanborn Field, a long-term research field at the University of Missouri, and Larry Mueller for help with field work NR 32 TC 6 Z9 6 U1 1 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD MAR-APR PY 2010 VL 102 IS 2 BP 734 EP 744 DI 10.2134/agronj2009.0206 PG 11 WC Agronomy SC Agriculture GA 576SJ UT WOS:000276168300046 ER PT J AU Wang, Y Seitz, HK Wang, XD AF Wang, Yan Seitz, Helmut K. Wang, Xiang-Dong TI Moderate Alcohol Consumption Aggravates High-Fat Diet Induced Steatohepatitis in Rats SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE Nonalcoholic Steatohepatitis; High-Fat Diet; Moderate Alcohol; Rats ID ETHANOL-INDUCED APOPTOSIS; NF-KAPPA-B; NONALCOHOLIC STEATOHEPATITIS; OXIDATIVE STRESS; LIVER-DISEASE; HEPATOCYTE APOPTOSIS; MOLECULAR-MECHANISMS; ACTIVATION; INJURY; RISK AB Background: Nonalcoholic steatohepatitis (NASH) develops in the absence of chronic and excessive alcohol consumption. However, it remains unknown whether moderate alcohol consumption aggravates liver inflammation in pre-existing NASH condition. Methods: Sprague-Dawley rats were first fed ad libitum with Lieber-DeCarli high-fat diet (71% energy from fat) for 6 weeks to induce NASH, as demonstrated previously. Afterwards, these rats were continuously fed with high-fat diet (HFD, 55% total energy from fat) or high fat plus alcohol diet (HFA, 55% energy from fat and 16% energy from alcohol) for an additional 4 weeks. Pathological lesions including fat accumulation and inflammatory foci in liver were examined and graded. Lipid peroxidation and apoptotic hepatocytes in the liver were assessed. The mRNA expressions of tumor necrosis factor-alpha (TNF alpha) and TNF receptor 1 (TNF-R1), Fas death receptor (Fas) and Fas ligant (FasL), IL-1 beta and IL-12 were determined by real-time PCR. Protein levels of total and cleaved caspase-3, CYP2E1, Bax, and Bcl-2 were measured by western blotting. Results: The number of hepatic inflammatory foci and apoptotic hepatocytes were significantly increased in rats fed with HFA as compared with those in HFD-fed rats. The aggravated inflammatory response and cellular apoptosis mediated by HFA were associated with elevated mRNA expression of Fas/FasL and cleaved caspase-3 protein. Although no significant differences were observed between HFD and HFA groups, the levels of lipid peroxidation, Bax and Bcl-2 protein concentration, and mRNA levels of other inflammatory cytokines were significantly higher in these 2 groups than those in the control group. Conclusions: These data suggest that even moderate alcohol consumption can cause more hepatic inflammation and cellular apoptosis in a pre-existing NASH condition. C1 [Wang, Yan; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Seitz, Helmut K.] Univ Heidelberg, Dept Med, Salem Med Ctr, D-6900 Heidelberg, Germany. [Seitz, Helmut K.] Univ Heidelberg, Lab Alcohol Res, Salem Med Ctr, D-6900 Heidelberg, Germany. RP Wang, XD (reprint author), Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu FU NIH [R01CA104932]; U. S. Department of Agriculture [1950-51000-064S] FX The work was supported by NIH grant R01CA104932 and U. S. Department of Agriculture, under agreement of NO 1950-51000-064S. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of National Institute of Health and the U. S. Department of Agriculture.; The authors thank Stephanie-Jo McGehee for her assistance in preparation of this manuscript. NR 42 TC 26 Z9 26 U1 1 U2 5 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD MAR PY 2010 VL 34 IS 3 BP 567 EP 573 DI 10.1111/j.1530-0277.2009.01122.x PG 7 WC Substance Abuse SC Substance Abuse GA 563OL UT WOS:000275142100021 PM 20028348 ER PT J AU Williamson, GB Wiemann, MC AF Williamson, G. Bruce Wiemann, Michael C. TI MEASURING WOOD SPECIFIC GRAVITY ... CORRECTLY SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE increment borers; moisture content; oven drying; tree cores; Wiemann approximation; wood density; wood specific gravity ID RAIN-FOREST TREES; FUNCTIONAL TRAITS; ABOVEGROUND BIOMASS; NEOTROPICAL FORESTS; AMAZONIAN FORESTS; BRAZILIAN AMAZON; TROPICAL TREES; DENSITY; GROWTH; DETERMINANTS AB The specific gravity (SG) of wood is a measure of the amount of structural material a tree species allocates to support and strength. In recent years, wood specific gravity, traditionally a forester's variable, has become the domain of ecologists exploring the universality of plant functional traits and conservationists estimating global carbon stocks. While these developments have expanded our knowledge and sample of woods, the methodologies employed to measure wood SG have not received as much scrutiny as SG's ecological importance. Here, we reiterate some of the basic principles and methods for measuring the SG of wood to clarify past practices of foresters and ecologists and to identify some of the prominent errors in recent studies and their consequences. In particular, we identify errors in (1) extracting wood samples that are not representative of tree wood, (2) differentiating wood specific gravity from wood density, (3) drying wood samples at temperatures below 100 degrees C and the resulting moisture content complications, and (4) improperly measuring wood volumes. In addition, we introduce a new experimental technique, using applied calculus, for estimating SG when the form of radial variation is known, a method that significantly reduces the effort required to sample a tree's wood. C1 [Williamson, G. Bruce] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Wiemann, Michael C.] US Forest Serv, Ctr Wood Anat Res, Forest Prod Lab, Madison, WI 53726 USA. RP Williamson, GB (reprint author), Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. EM btwill@lsu.edu FU U.S. National Science Foundation [DEB-0639114] FX This research was supported by the U.S. National Science Foundation (DEB-0639114). E. Alec Johnson helped with the calculus, and Sayra Navas Ocampo translated the abstract. R. Chazdon and F. Bongers commented on the manuscript. NR 52 TC 96 Z9 98 U1 4 U2 40 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD MAR PY 2010 VL 97 IS 3 BP 519 EP 524 DI 10.3732/ajb.0900243 PG 6 WC Plant Sciences SC Plant Sciences GA 563DF UT WOS:000275106800014 PM 21622413 ER PT J AU Thame, MM Fletcher, HM Baker, TM Jahoor, F AF Thame, Minerva M. Fletcher, Horace M. Baker, Tameka M. Jahoor, Farook TI Comparing the glucose kinetics of adolescent girls and adult women during pregnancy SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID INFANT BIRTH-WEIGHT; CARBOHYDRATE-METABOLISM; LONGITUDINAL CHANGES; BODY-COMPOSITION; MATERNAL GROWTH; MATURE WOMEN; GLUCONEOGENESIS; INSULIN AB Background: Fetal energy demands are met mostly from oxidation of maternally supplied glucose. In pregnant adults this increased glucose requirement is met by an increase in gluconeogenesis. It is not known, however, whether, like their adult counterparts, pregnant adolescent girls can increase gluconeogenesis-hence, glucose production. Objective: Our objective was to measure glucose kinetics in 8 pregnant adolescents and 8 adult women. Design: We measured glucose kinetics after an overnight fast by using a primed-constant 6-h U-C-13-glucose infusion at the end of trimester 1 and early trimester 3. Results: From trimester 1 to trimester 3, whole-body glucose production increased significantly in both groups (P < 0.01). However, whereas the weight-specific rate in adults increased by 18.2%, it increased by only 14.3% in adolescents. In adults, the increase in whole-body glucose production was largely due to a significant increase (P < 0.01) in the rate of gluconeogenesis, but in adolescents there was no change in whole-body gluconeogenesis, and weight-specific gluconeogenesis actually decreased by 11.7%. In both groups, the rate of whole-body glycogenolysis increased significantly (P < 0.05) in trimester 3, and in adolescents, it increased by 95%. Conclusions: These findings suggest that, in the fasted state in late pregnancy, pregnant adolescents cannot increase weight-specific glucose production by the same magnitude as their adult counterparts. Furthermore, whereas adult women increase glucose production primarily through gluconeogenesis, adolescents do so through glycogenolysis. Am J Clin Nutr 2010;91:604-9. C1 [Jahoor, Farook] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Thame, Minerva M.; Fletcher, Horace M.; Baker, Tameka M.] Univ W Indies, Dept Obstet Gynaecol & Child Hlth, Kingston 7, Jamaica. RP Jahoor, F (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM fjahoor@bcm.edu FU USDA/Agricultural Research Service [58-6250-6001] FX Supported by the USDA/Agricultural Research Service (cooperative agreement no. 58-6250-6001). NR 21 TC 7 Z9 7 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 2010 VL 91 IS 3 BP 604 EP 609 DI 10.3945/ajcn.2009.28635 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 557YM UT WOS:000274706500016 PM 20089733 ER PT J AU Yeum, KJ Orioli, M Regazzoni, L Carini, M Rasmussen, H Russell, RM Aldini, G AF Yeum, Kyung-Jin Orioli, Marica Regazzoni, Luca Carini, Marina Rasmussen, Helen Russell, Robert M. Aldini, Giancarlo TI Profiling histidine dipeptides in plasma and urine after ingesting beef, chicken or chicken broth in humans SO AMINO ACIDS LA English DT Article DE Carnosine; Anserine; Histidine-dipeptides; Absorption kinetic; Chicken ID TANDEM MASS-SPECTROMETRY; CONTAINING PEPTIDES; SERUM CARNOSINASE; MICHAEL ADDUCTS; MUSCLE; ANSERINE; TISSUES; HOMOCARNOSINE; CONSTITUENTS; ABSORPTION AB The in vitro metabolic stability of histidine-dipeptides (HD), carnosine (CAR) and anserine (ANS), in human serum, and their absorption kinetics after ingesting pure carnosine or HD rich foods in humans have been investigated. Healthy women (n = 4) went through four phases of taking one dose of either 450 mg of pure carnosine, 150 g beef (B), 150 g chicken (C), or chicken broth (CB) from 150 g chicken with a > 2-week washout period between each phase. Blood samples were collected at 0, 30, 60, 100, 180, 240, and 300 min, and urine samples before and after (up to 7 h) ingesting pure carnosine or food. Both plasma and urine samples were analyzed for HD concentrations using a sensitive and selective LC-ESI-MS/MS method. CAR was undetectable in plasma after ingesting pure carnosine, B, C or CB. By contrast, plasma ANS concentration was significantly increased (P < 0.05) after ingesting C or CB, respectively. Urinary concentrations of both CAR and ANS were 13- to 14-fold increased after ingesting B, and 14.8- and 243-fold after CB ingestion, respectively. Thus, dietary HD, which are rapidly hydrolyzed by carnosinase in plasma, and excreted in urine, may act as reactive carbonyl species sequestering agents. C1 [Yeum, Kyung-Jin; Rasmussen, Helen] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Orioli, Marica; Regazzoni, Luca; Carini, Marina; Aldini, Giancarlo] Univ Milan, Fac Pharm, Dept Pharmaceut Sci, I-20133 Milan, Italy. [Russell, Robert M.] NIH, Off Director, Washington, DC USA. RP Yeum, KJ (reprint author), Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM kyungjin.yeum@tufts.edu RI orioli, marica/F-7606-2015; Regazzoni, Luca/D-4212-2014; CARINI, MARINA/D-3084-2015 OI orioli, marica/0000-0003-1558-9551; Regazzoni, Luca/0000-0001-7199-7141; aldini, giancarlo/0000-0002-2355-6744; CARINI, MARINA/0000-0003-3407-5425 FU U. S. Department of Agriculture [581950-9-001] FX This research has been supported by the U. S. Department of Agriculture, under agreement number 581950-9-001. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U. S. Department of Agriculture. NR 35 TC 18 Z9 21 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0939-4451 J9 AMINO ACIDS JI Amino Acids PD MAR PY 2010 VL 38 IS 3 BP 847 EP 858 DI 10.1007/s00726-009-0291-2 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 569WT UT WOS:000275634200019 PM 19381778 ER PT J AU Demeke, T Jenkins, GR AF Demeke, Tigst Jenkins, G. Ronald TI Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Review DE DNA extraction; Sample matrices; DNA quantification; PCR inhibitors; Variability of DNA yield ID GENETICALLY-MODIFIED ORGANISMS; QUANTITATIVE PCR; AGRICULTURAL BIOTECHNOLOGY; METROLOGICAL TRACEABILITY; GM QUANTIFICATION; FRAGMENT RATIOS; ANALYTICAL TOOL; MODIFIED MAIZE; FOOD SAMPLES; GENOMIC DNA AB Biotechnology-derived varieties of canola, cotton, corn and soybean are being grown in the USA, Canada and other predominantly grain exporting countries. Although the amount of farmland devoted to production of biotechnology-derived crops continues to increase, lingering concerns that unintended consequences may occur provide the EU and most grain-importing countries with justification to regulate these crops. Legislation in the EU requires traceability of grains/oilseeds, food and feed products, and labelling, when a threshold level of 0.9% w/w of genetically engineered trait is demonstrated to be present in an analytical sample. The GE content is routinely determined by quantitative PCR (qPCR) and plant genomic DNA provides the template for the initial steps in this process. A plethora of DNA extraction methods exist for qPCR applications. Implementing standardized methods for detection of genetically engineered traits is necessary to facilitate grain marketing. The International Organization for Standardization draft standard 21571 identifies detergent-based methods and commercially available kits that are widely used for DNA extraction, but also indicates that adaptations may be necessary depending upon the sample matrix. This review assesses advantages and disadvantages of various commercially available DNA extraction kits, as well as modifications to published cetyltrimethylammonium bromide methods. Inhibitors are a major obstacle for efficient amplification in qPCR. The types of PCR inhibitors and techniques to minimize inhibition are discussed. Finally, accurate quantification of DNA for applications in qPCR is not trivial. Many confounders contribute to differences in analytical measurements when a particular DNA quantification method is applied and different methods do not always provide concordant results on the same DNA sample. How these differences impact measurement uncertainty in qPCR is considered. C1 [Demeke, Tigst] Canadian Grain Commiss, Grain Res Lab, Winnipeg, MB R3C 3G8, Canada. [Jenkins, G. Ronald] Grain Inspect Packers & Stockyards Adm, USDA, Kansas City, MO 64153 USA. RP Demeke, T (reprint author), Canadian Grain Commiss, Grain Res Lab, 1404-303 Main St, Winnipeg, MB R3C 3G8, Canada. EM tigst.demeke@grainscanada.gc.ca NR 75 TC 109 Z9 114 U1 9 U2 104 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD MAR PY 2010 VL 396 IS 6 BP 1977 EP 1990 DI 10.1007/s00216-009-3150-9 PG 14 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 567NW UT WOS:000275454900005 PM 19789856 ER PT J AU Holden, MJ Levine, M Scholdberg, T Haynes, RJ Jenkins, GR AF Holden, Marcia J. Levine, Marci Scholdberg, Tandace Haynes, Ross J. Jenkins, G. Ronald TI The use of 35S and Tnos expression elements in the measurement of genetically engineered plant materials SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Nucleic acids (DNA); PCR; CaMV 35S promoter; Tnos; Quantitative real-time PCR; Biotechnological products ID POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; CAULIFLOWER MOSAIC-VIRUS; MODIFIED MAIZE; QUANTIFICATION; PROMOTER; QUANTITATION; VALIDATION; FOOD AB An online survey was conducted by the International Life Sciences Institute, Food Biotechnology Committee, on the use of qualitative and quantitative polymerase chain reaction (PCR) assays for cauliflower mosaic virus 35S promoter and Agrobacterium tumefaciens Tnos DNA sequence elements for the detection of genetically engineered (GE) crop plant material. Forty-four testing laboratories around the world completed the survey. The results showed the widespread use of such methods, the multiplicity of published and in-house methods, and the variety of reference materials and calibrants in use. There was an interest on the part of respondents in validated quantitative assays relevant to all GE events that contain these two genetic elements. Data are presented by testing two variations each of five published real-time quantitative PCR methods for 35S detection on eight maize reference materials. The results showed that two of the five methods were not suitable for all the eight reference materials, with poor linear regression parameters and multiple PCR amplification products for some of the reference materials. This study demonstrates that not all 35S methods produce satisfactory results, emphasizing the need for method validation. C1 [Holden, Marcia J.; Haynes, Ross J.] NIST, Div Biochem Sci, Gaithersburg, MD 20899 USA. [Levine, Marci] ILSI, IFBiC, Washington, DC 20005 USA. [Scholdberg, Tandace; Jenkins, G. Ronald] GIPSA, TSD, USDA, Kansas City, MO 64153 USA. RP Holden, MJ (reprint author), NIST, Div Biochem Sci, 100 Bur Dr, Gaithersburg, MD 20899 USA. EM marcia.holden@nist.gov FU NIST of Wade Reimonenq of Southern University A&M College, Baton Rouge, LA; BASF; Bayer CropScience; Cargill Inc.; Dow AgroSciences; Monsanto Company; Masterfoods, Inc.; Pioneer; A Dupont Company; Procter & Gamble Company, Inc.; Syngenta Biotechnology, Inc.; CropLife International through an agreement with NIST and GIPSA FX We wish to thank the 44 laboratories who completed the questionnaire. We acknowledge the fine technical support at the NIST of Wade Reimonenq of Southern University A&M College, Baton Rouge, LA.; Support The survey described in this manuscript was supported by the members of the ILSI International Food Biotechnology Committee: BASF; Bayer CropScience; Cargill Inc., Dow AgroSciences; Monsanto Company; Masterfoods, Inc., Pioneer, A Dupont Company; and Procter & Gamble Company, Inc., Syngenta Biotechnology, Inc. CropLife International provided support for this project through an agreement with NIST and GIPSA. NR 26 TC 10 Z9 15 U1 0 U2 7 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD MAR PY 2010 VL 396 IS 6 BP 2175 EP 2187 DI 10.1007/s00216-009-3186-x PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 567NW UT WOS:000275454900022 PM 19856176 ER PT J AU Farmer, C Palin, MF Gilani, GS Weiler, H Vignola, M Choudhary, RK Capuco, AV AF Farmer, C. Palin, M. F. Gilani, G. S. Weiler, H. Vignola, M. Choudhary, R. K. Capuco, A. V. TI Dietary genistein stimulates mammary hyperplasia in gilts SO ANIMAL LA English DT Article; Proceedings Paper CT International Conference on Livestock and Global Climate Change CY MAY, 2008 CL Hammamet, TUNISIA SP British Soc Anim Sci, INRA, European Assoc Anim Prod, ICARDA DE genistein; mammary glands; phytoestrogen; pigs; puberty ID GROWTH-FACTOR-I; BREAST-CANCER; PHYTOESTROGEN GENISTEIN; EGF-RECEPTOR; GLAND; ISOFLAVONES; ESTROGEN; EXPRESSION; EXPOSURE; BONE AB The possible role of the phytoestrogen genistein on prepubertal development of mammary glands, hormonal status and bone resorption was investigated in gilts. Forty-five gilts were fed a control diet containing soya (CTLS, n = 15), a control diet without soya (CTL0, n = 15) or the CTLS diet supplemented with 2.3 g of genistein daily (GEN, n = 15) from 90 days of age until slaughter (day 183 +/- 1). Both basal diets were isonitrogenous and isocaloric. Jugular blood samples were obtained on days 89 and 176 to determine concentrations of isoflavone metabolites (on day 176 only), prolactin, estradiol, progesterone, insulin-like growth factor 1 (IGF1), and N-telopeptide of type I Collagen (NTx; on day 176 only). At slaughter, mammary glands were excised, parenchymal and extraparenchymal tissues were dissected, and composition of parenchymal tissue (protein, fat, dry matter (DM), DNA) was determined. Histochemical analyses of mammary parenchyma were performed. Dietary genistein increased parenchymal protein (P < 0. 05) while decreasing DM (P < 0. 05) and tending to lower fat content compared with the CTLS, but not the CTL0, diet. There was more parenchymal DNA (1.26 v. 0.92 mg/g, P < 0. 05) in GEN than CTLS gilts, likely reflecting an increase in the quantity of mammary epithelial cells. Circulating concentrations of genistein were increased in GEN gilts (P < 0. 00 1) but concentrations of hormones or NTx (indicator of bone collagen resorption) were not affected by GEN (P > 0. 1). Percentage of estradiol receptor alpha (ER alpha)-positive epithelial cells was lower (P < 0.05) in GEN than CTLS gilts, whereas 5-bromo-2'-deoxyuridine labeling index was unaltered (P > 0. 1). Transcript levels for ER alpha, ERG, IGF1, epidermal growth factor (EGF), epidermal growth factor receptor and transforming growth factor alpha were not altered by treatments. Supplementation of the diet with genistein during the growing phase in gilts, therefore, led to hyperplasia of mammary parenchymal tissue after puberty; yet, even though circulating genistein was increased, this was not accompanied by changes in mammary expression of selected genes or circulating hormone levels. C1 [Farmer, C.; Palin, M. F.] Agr & Agri Food Canada, Dairy & Swine Res & Dev Ctr, Lennoxville STN, Sherbrooke, PQ J1M 1Z3, Canada. [Gilani, G. S.] Hlth Canada, Nutr Res Div, Ottawa, ON K1A 0L2, Canada. [Weiler, H.] McGill Univ, Sch Dietet & Human Nutr, Ste Anne De Bellevue, PQ H9X 3V9, Canada. [Vignola, M.] NUTRECO, Brossard, PQ J4W 3E7, Canada. [Choudhary, R. K.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Capuco, A. V.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. RP Farmer, C (reprint author), Agr & Agri Food Canada, Dairy & Swine Res & Dev Ctr, Lennoxville STN, POB 90, Sherbrooke, PQ J1M 1Z3, Canada. EM Chantal.Farmer@agr.gc.ca NR 51 TC 7 Z9 7 U1 0 U2 1 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 J9 ANIMAL JI Animal PD MAR PY 2010 VL 4 IS 3 BP 454 EP 465 DI 10.1017/S1751731109991200 PG 12 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 564YJ UT WOS:000275254400014 PM 22443950 ER PT J AU Cox, F Barsoum, N Bidartondo, MI Borja, I Lilleskov, E Nilsson, LO Rautio, P Tubby, K Vesterdal, L AF Cox, Filipa Barsoum, Nadia Bidartondo, Martin I. Borja, Isabella Lilleskov, Erik Nilsson, Lars O. Rautio, Pasi Tubby, Kath Vesterdal, Lars TI A leap forward in geographic scale for forest ectomycorrhizal fungi SO ANNALS OF FOREST SCIENCE LA English DT Letter DE biogeography; ICP Forests; function; mycorrhizas; symbiosis ID BELOW-GROUND VIEWS; NITROGEN DEPOSITION; COMMUNITY STRUCTURE; PINUS-SYLVESTRIS; CLIMATE-CHANGE; ELEVATED CO2; CARBON; DIVERSITY; RESPONSES; SEEDLINGS C1 [Cox, Filipa; Bidartondo, Martin I.] Royal Bot Gardens, Richmond TW9 3DS, England. [Cox, Filipa; Bidartondo, Martin I.] Univ London Imperial Coll Sci Technol & Med, London SW7 2AZ, England. [Cox, Filipa; Barsoum, Nadia; Tubby, Kath] Alice Holt Lodge, Forest Res, Farnham GU10 4LH, Surrey, England. [Borja, Isabella] Norwegian Forest & Landscape Inst, N-1431 As, Norway. [Lilleskov, Erik] US Forest Serv, USDA, No Res Stn, Houghton, MI 49901 USA. [Nilsson, Lars O.; Vesterdal, Lars] Univ Copenhagen, DK-2970 Horsholm, Denmark. [Rautio, Pasi] Finnish Forest Res Inst, Rovaniemi Res Unit, Rovaniemi, Finland. RP Cox, F (reprint author), Royal Bot Gardens, Richmond TW9 3DS, England. EM f.cox06@imperial.ac.uk RI Vesterdal, Lars/D-5227-2011; Rautio, Pasi/A-6595-2013; Borja, Isabella/A-8496-2010; Section, Forest Health/B-1469-2015 OI Vesterdal, Lars/0000-0003-0309-3735; Rautio, Pasi/0000-0003-0559-7531; Borja, Isabella/0000-0002-3740-9948; NR 33 TC 5 Z9 8 U1 2 U2 29 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1286-4560 EI 1297-966X J9 ANN FOREST SCI JI Ann. For. Sci. PD MAR-APR PY 2010 VL 67 IS 2 AR 200 DI 10.1051/forest/2009107 PG 6 WC Forestry SC Forestry GA 581FN UT WOS:000276507100001 ER PT J AU Elkinton, JS Boettner, GH Sermac, M Gwiazdowski, R Hunkins, RR Callahan, J Scheufele, SB Donahue, CP Porter, AH Khrimian, A Whited, BM Campbell, NK AF Elkinton, Joseph S. Boettner, George H. Sermac, Marinko Gwiazdowski, Rodger Hunkins, Roy R. Callahan, Julie Scheufele, Susan B. Donahue, Charlene P. Porter, Adam H. Khrimian, Ashot Whited, Brenda M. Campbell, Nichole K. TI Survey for Winter Moth (Lepidoptera: Geometridae) in Northeastern North America With Pheromone-Baited Traps and Hybridization With the Native Bruce Spanworm (Lepidoptera: Geometridae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE forest defoliator; hybridization; invasive species; pheromone trap survey; DNA barcoding ID OPEROPHTERA-BRUMATA L; NOVA-SCOTIA; EPIRRITA-AUTUMNATA; BIOLOGICAL-CONTROL; HULST LEPIDOPTERA; APPLE ORCHARDS; SEX-PHEROMONE; POPULATIONS; DNA; PARASITISM AB We used pheromone-baited traps to survey the distribution of winter moth, Operophtera brumata (L.) (Lepidoptera: Geometridae), a new invasive defoliator from Europe in eastern New England. The traps also attracted Bruce spanworm, Operophtera bruceata (Hulst) (Lepidoptera: Geometridae), native to North America. We distinguished between the two species by examining male genitalia and sequencing the mitochondrial cytochrome oxidase subunit I (COI) gene, the DNA barcoding region. In 2005, we recovered winter moths at sites stretching from eastern Long Island, southeastern Connecticut, all of Rhode Island, eastern Massachusetts, coastal New Hampshire, and southern coastal Maine, At sites further west and north we captured only Bruce spanworm. In 2006, we confirmed that both winter moth and Bruce spanworm are present in Nova Scotia and in coastal Maine, bill only Bruce spanworm was recovered in coastal New Brunswick, Canada; Pennsylvania; Vermont; or Quebec City, Canada. In 2007, we collected Bruce spanworm, bill no winter moths, in New Brunswick and the interior areas of Maine, New Hampshire, and New York. Winter, moth and Bruce spanworm differed in the COI sequence by 7.45% of their nucleotides. The prevalence of intermediate genitalia in the zone of overlap suggested that hybridization between the two species may be occurring. To confirm the presence of hybrids, we sequenced the nuclear gene, glucose-6-phosphate dehydrogenase (C6PD). We, identified six nucleotides that routinely distinguished winter moth and Bruce spanworm, of which three were always diagnostic. We showed that eggs produced by hybridizing the two species in the laboratory contained copies of both species at these six sites. We found that most of the moths collected in the field with intermediate genitalia had winter moth COI and GOD sequences and thus were not hybrids (or at least F1 hybrids). We found three hybrids out of 158 moths with intermediate genitalia in the region both species were caught. We conclude that hybrids occur in nature, but are not its common as previously reported. Introgression of genes between the two species may still be significant. C1 [Elkinton, Joseph S.; Boettner, George H.; Sermac, Marinko; Gwiazdowski, Rodger; Hunkins, Roy R.; Scheufele, Susan B.; Porter, Adam H.] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA. [Elkinton, Joseph S.; Gwiazdowski, Rodger; Porter, Adam H.; Whited, Brenda M.] Univ Massachusetts, Grad Program Organism & Evolut, Amherst, MA 01003 USA. [Callahan, Julie] Univ Massachusetts, Massachusetts Dept Agr Resources, Amherst, MA 01003 USA. [Donahue, Charlene P.] Maine Forest Serv, Maine Dept Conservat Insect & Dis Lab, Augusta, ME 04330 USA. [Khrimian, Ashot] USDA ARS, Beltsville, MD 20705 USA. [Campbell, Nichole K.] PSS CT MA RI USDA APHIS PPQ, Wallingford, CT 06492 USA. RP Elkinton, JS (reprint author), Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA. EM elkinton@ent.umass.edu FU U.S. Department of Agriculture's Animal and Plant Health Inspection Service (APHIS) [05-8225-0464-CA]; USDA Forest Service [04-CA-11244225-414]; Massachusetts State Legislature FX We thank the following people for deploying and retrieving the pheromone traps and for sending Lis the moths from the stated locations: Massachusetts: C. Burnham, K, Gooch, G Witkus; Maine: K. Coluzzi, J. Crowe, M. Skinner, G. Smith, W. Urquhart Vermont: B. Burns, J. Esden, T. Greaves. T. Hanson, R. Kelly, L. Lund, T. Simmons; New York: K. Carnes, P. Jentsch, D. Gilrein, H. McGinnis; Connecticut: V. Smith, P. Trenchard, D. Ellis; Rhode Island: S. Baxter, H. Faubert, C. Sparks, D. Martin; New Hampshire: C. Tatum, J. Weaver; Pennsylvania: J. Stimmel, S. Gardosik, Sven Spichiger New Jersey: S. Vaiciunas, R. Fine, J. Simmons; Wisconsin: A. Diss-Torrance, B. Schwingle, L. Williams; Michigan: D. McCullough; Minnesota: D. Zumeta, A. Jones; British Columbia: L. Humble, G. Zilahi-Balogh; New Brunswick: R. Webster: Ontario: Ba. Lyons, Be Lyons; Quebec: J. Regniere, P. Duval; Austria: G. Hoch: and United Kingdom: C. Tilbury, A. Vanbergen, A. Watt, We thank I. Otvos and N. Condor for sending us winter moth pupae from British Columbia: A. Liebhold D. Embree and D. Souto for critical reviews; and V. Neilis for querying the Canadian Forest Insect and Disease survey regarding winter moth in New Brunswick. We thank Gloria Witkus for help in the field and with dissections, Natalie Leva for mailing traps to our cooperators, Jeremey Anderson for assisting with GenBank submissions, Jeff Ahern for assistance ill initiating the DNA analysis, and Maili Page and Diana Barsez for assistance ill producing maps. This research was made possible, in part, by it cooperative agreement (05-8225-0464-CA) from the U.S. Department of Agriculture's Animal and Plant Health Inspection Service (APHIS). We are also grateful to the USDA Forest Service (cooperative agreement 04-CA-11244225-414), and a grant from the Massachusetts State Legislature for supporting this work. NR 24 TC 16 Z9 17 U1 2 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 135 EP 145 DI 10.1603/AN09118 PG 11 WC Entomology SC Entomology GA 566QC UT WOS:000275386800003 ER PT J AU Harris, JW Danka, RG Villa, JD AF Harris, Jeffrey W. Danka, Robert G. Villa, Jose D. TI Honey Bees (Hymenoptera: Apidae) With the Trait of Varroa Sensitive Hygiene Remove Brood With All Reproductive Stages of Varroa Mites (Mesostigmata: Varroidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE honey bees; varroa; varroa sensitive hygiene; hygiene ID APIS-MELLIFERA; DESTRUCTOR MITES; UNITED-STATES; BEHAVIOR; RESISTANCE; JACOBSONI; COLONIES; DISEASE; CELLS; RESPONSES AB Varroa sensitive hygiene (VSH) is a trait of honey bees, Apis mellifera L. (Hymenoptera: Apidae), which supports resistance to Varroa destructor Anderson & Trueman. VSH is the hygiene removal of mite-infested pupa. Bees selectively bred for VSH produce colonies in which the fertility of mites decreases over time. In addition, mite fertility decreases after infested brood is exposed to VSH bees for 1 wk. The purpose of this study Was to decide whether the reduction in mite fertility is caused by selective removal of mites that produce offspring. Initially, we monitored changes in a small patch of capped brood during exposure to VSH bees at 2-h intervals through 60 h, which provided a reference for the subsequent experiment. The first test showed that VSH bees uncapped, recapped, and began to remove many pupae in approximate to 2 h. The approach in the second experiment was to compare the percentage of fertile mites from brood exposed to VSH bees for a 3-h period to the percentage of fertile mites in brood that was protected front hygiene by a screen. There were no significant differences in fertility between Mites Oil pupae that were being removed by the bees and mites oil protected pupae. These results suggest that neither egg-laying by foundress mites nor mite offspring are the stimuli that trigger hygienic removal of mite-infested pupae by VSH bees. It may he that hygienic activities such as the uncapping of brood cells inhibits or disrupts reproduction by varroa mites. C1 [Harris, Jeffrey W.; Danka, Robert G.; Villa, Jose D.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. RP Harris, JW (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM jeffrey.harris@ars.usda.gov NR 29 TC 16 Z9 16 U1 2 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 146 EP 152 DI 10.1603/AN09138 PG 7 WC Entomology SC Entomology GA 566QC UT WOS:000275386800004 ER PT J AU Moran, PJ Goolsby, JA AF Moran, Patrick J. Goolsby, John A. TI Biology of the Armored Scale Rhizaspidiotus donacis (Hemiptera: Diaspididae), a Candidate Agent for Biological Control of Giant Reed SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE armored scale; biology; biological control; non-native invasive grass; giant reed ID WHITE PEACH SCALE; PSEUDAULACASPIS-PENTAGONA; TETRAMESA-ROMANA; NORTH-AMERICA; HOST-RANGE; HOMOPTERA; INSECTS; GROWTH; L.; CALIFORNIA AB The development, survival, and reproductive output of the armored scale Rhizaspidiotus donacis Leonardi (Hemiptera: Diaspididae) were determined as part of an evaluation of this scale for biological control of giant reed, Arundo donax L. Scale crawlers produced by females collected in southern France and Spain lived for less than two d in the absence of a host. on A. donax shoots held under a diurnally variable temperature regime (15-26 degrees C), crawlers settled on leaf collars and axillary stem bases and completed the first instar within 14 d. Winged adult males emerged within six wk Old lived 1.7 d in vials. Immobile adult females were observed 75 d after crawler release. Development time to adult was reduced 15% in males and 28% in Females when scales were reared at a constant temperature of 26 degrees C. Survival from crawler to adult was 20-25%. The generation time for laboratory-reared females was 170 d and their average life span was 203 d. Females from field collections produced an average of 85 live crawlers. 2.7-fold more than did colony-reared females. Across 31 field collections, female size was correlated to the percentage of scales that were reproductive. However, among eight collections from two Spanish sites, size varied but was not correlated to reproductive Success. Peak reproduction from field collections occurred between November and March, and the scale complete-cl two generations per year. Biological information about R. donacis will aid production, release, and field evaluation of this scale for biological control of invasive A. donax in North America. C1 [Moran, Patrick J.; Goolsby, John A.] ARS, USDA, Beneficial Insects Res Unit, Weslaco, TX 78596 USA. RP Moran, PJ (reprint author), ARS, USDA, Beneficial Insects Res Unit, 2413 E Highway 83, Weslaco, TX 78596 USA. EM patrick.moran@ars.usda.gov FU U.S. Department of Homeland Security, Science and Technology Directorate FX We thank Alan Kirk (USDA-ARS, European Biological Control Laboratory, Montpellier, France [retired]), Elena Cortes and M. A. Marcos (Biodiversity Research Institute, University of Alicante, Spain) for collecting scales in Europe and Greg Evans (USDA-ARS, Systematic Entomology Laboratory, Washington, DC) for scale parasite identification. We thank Don Sands (Commonwealth Scientific and Industrial Research Organization-Entomology, Queensland, Australia) for critical advice on rearing and biological evaluation of armored scales. We thank Crystal Salinas. Ann Vacek and Sandra Espinoza (USDA-ARS, Weslaco, TX) for technical assistance, Kathy Yeater (USDA-ARS, Hilo, HI) and, A. Kirk for helpful reviews. This work was supported in part by a grant from the U.S. Department of Homeland Security, Science and Technology Directorate. NR 52 TC 11 Z9 13 U1 1 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 252 EP 263 DI 10.1603/AN09124 PG 12 WC Entomology SC Entomology GA 566QC UT WOS:000275386800014 ER PT J AU Leopold, RA Rajamohan, A Shelly, TE Handler, AM AF Leopold, Roger A. Rajamohan, Arun Shelly, Todd E. Handler, Alfred M. TI Quality Testing of Three Species of Tephritid Fruit Flies After Embryo Cryopreservation SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Anastrepha ludens; Anastrepha suspensa; Ceratitis capitata; long-term cold storage; fitness testing ID DROSOPHILA-MELANOGASTER; GENETIC-VARIATION; FLY; VITRIFICATION; OOCYTES; DIPTERA; STABILITY; LONGEVITY; SELECTION; SURVIVAL AB This study evaluates characteristics commonly used to define insect quality or fitness by using a complement of three species of tephritid fruit flies (Diptera: Tephritidae) obtained from cryopreserved embryos. Anastrepha ludens (Loew), Anastrepha suspensa (Loew), and Ceratitis capitata (Wiedemann) were used to assess embryo to adult emergence and adult longevity, flight ability mating ability, fecundity, and genetic variability after cryopreservation. With the three species tested: embryo survival was reduced by 50-70% whereas adult eclosion seemed unaffected by cryogenic treatment. Laboratory cage survival of cryopreserved A. ludens paralleled that of controls when tested with or without food and water posttreatment. With C. capitata, field cage survival was also similar when the adult progeny of cryopreserved parents was compared with that Of Untreated flies of the same age, Assessment of flight ability of cryopreserved A. ludens over a 19-d period by using a flight mill showed no statistical difference when compared with the untreated groups Over the same time period. Flight ability within field cages for newly emerged progeny of cryopreserved C. capitata also mirrored that of the controls. Observed matings occurring within laboratory cages containing equal numbers of A. ludens males and females did not differ from comparable control groups, Furthermore, male progeny obtained from cryopreserved C. capitata parents competed equally With untreated males for mates while housed in field cages. A laboratory analysis of Fertility and fecundity of A. suspensa revealed that males mated with control females were unaffected by cryopreservation as embryos. whereas cryopreserved females exhibited a significantly reduced fecundity when mated with control males. The fecundity of C. capitata progeny of cryopreserved parents also did not differ from control levels while caged Under laboratory conditions. A random amplified polymorphic DNA assay of the genetic diversity of A. ludens comparing cryopreserved males with control males showed that the coefficient of similarity was >= 85%. This study indicates that embryo cryopreservation had little or no effect on the reproduction, longevity and flight of the species tested and can be used to support maintenance of insect stocks and control programs supported by the mass-rearing process. C1 [Leopold, Roger A.] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Rajamohan, Arun] N Dakota State Univ, Dept Entomol, Fargo, ND 58105 USA. [Shelly, Todd E.] USDA, APHIS, Fruit Fly Genet & Rearing Lab, Waimanalo, HI 96795 USA. [Handler, Alfred M.] Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Leopold, RA (reprint author), USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. EM roger.leopold@ars.usda.gov NR 41 TC 1 Z9 1 U1 1 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 264 EP 272 DI 10.1603/AN09094 PG 9 WC Entomology SC Entomology GA 566QC UT WOS:000275386800015 ER PT J AU Nagoshi, RN AF Nagoshi, Rodney N. TI The Fall Armyworm Triose Phosphate Isomerase (Tpi) Gene as a Marker of Strain Identity and Interstrain Mating SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Spodoptera frugiperda; host strains; triose phosphate isomerase ID NOCTUIDAE HOST STRAINS; SPODOPTERA-FRUGIPERDA LEPIDOPTERA; FRAGMENT-LENGTH-POLYMORPHISMS; MOLECULAR CHARACTERIZATION; I GENE; POPULATIONS; IDENTIFICATION; BRAZIL; SEQUENCE; INTRONS AB Fall armyworm, Spodoptera frugiperda (J.E. Smith), is a significant agricultural pest in the United States, affecting most notably sweet corn, Zea malls L., and turfgrass, Two strains exist that differ physiologically and behaviorally but are morphologically indistinguishable, Polymorphisms within the fall armyworm Triose phosphate isomerase gene (Tpi; EC 5.3.1.1) were characterized and used to develop a polymerase chain reaction-based method for discriminating fall armyworm strains. The Tpi markers were used in multilocus analysis to estimate interstrain hybrid frequency and their distribution in populations from the United States and Brazil. The results were compared with previous Studies using different marker combinations to test hypotheses about interstrain mating behavior and to explain earlier observations of unusual Strain distribution patterns. The importance of understanding the frequency and consequences of interstrain hybridization to deciphering the mechanism of Strain divergence and the distribution of fall armyworm subpopulations in different habitats are discussed. C1 USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Nagoshi, RN (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov NR 29 TC 13 Z9 13 U1 0 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 283 EP 292 DI 10.1603/AN09046 PG 10 WC Entomology SC Entomology GA 566QC UT WOS:000275386800017 ER PT J AU Brent, CS AF Brent, C. S. TI Reproductive Refractoriness in the Western Tarnished Plant Bug (Hemiptera: Miridae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Lygus hesperus; postmating refractory period; sexual receptivity; spermatophore ID JUVENILE-HORMONE; LYGUS-HESPERUS; LYGOCORIS-PABULINUS; MATED FEMALE; BEHAVIOR; INSECTS; BIOSYNTHESIS; HETEROPTERA; COPULATION; BIOLOGY AB Adult Lygus hesperus Knight (Hemiptera: Miridae), major crop pests of the south-western United States, can mate repeatedly during their lives, potentially enhancing the lifetime fitness of both sexes. However, the periodicity of mating and its regulation in this species is poorly understood. In this study the postmating refractory period for both sexes is described. Males were found to have a median delay of 24 h, which seems to be the minimum time needed to refill the accessory glands and produce another viably sized spermatophore. The median refractory period in mated females was 5 d, but sometimes lasted longer than 2 wk. The loss of sexual receptivity in mated females, at least for the first 24 h, seems to be induced by males; spermatophore homogenates injected directly into the abdomen of virgin females reduced their sexual receptivity. The total duration of female refractoriness may be dictated by the starting size of a spermatophore and the rate at which it degrades. C1 USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. RP Brent, CS (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85238 USA. EM colin.brent@ars.usda.go NR 34 TC 10 Z9 10 U1 0 U2 5 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2010 VL 103 IS 2 BP 300 EP 306 DI 10.1603/AN09135 PG 7 WC Entomology SC Entomology GA 566QC UT WOS:000275386800019 ER PT J AU Navajas, M Anderson, DL de Guzman, LI Huang, ZY Clement, J Zhou, T Le Conte, Y AF Navajas, Maria Anderson, Denis L. de Guzman, Lilia I. Huang, Zachary Y. Clement, Jeremy Zhou, Ting Le Conte, Yves TI New Asian types of Varroa destructor: a potential new threat for world apiculture SO APIDOLOGIE LA English DT Article DE Apis mellifera; Apis cerana; Varroa; mitochondrial DNA; diversity ID APIS-MELLIFERA COLONIES; HONEY-BEES; SEQUENCE ALIGNMENT; JACOBSONI; MITES; ACARI; IDENTIFICATION; HAPLOTYPE; AMERICA; CERANA AB The invasion of the Western honey bee, Apis mellifera, by Varroa destructor is attributed to two mitochondrial haplotypes (K and J) that shifted last century from their primary host the Eastern honey bee, A. cerana, in north-east Asia. Here, mitochondrial DNA sequences (cox1, cox3, atp6 and cytb: 2700 base pairs) were obtained from mites infesting both Eastern and Western honeybees (respectively 21 and 11 colonies) from Asia including regions where the shifts first occurred. A total of eighteen haplotypes were uncovered in Asia (11 on A. cerana and 7 on A. mellifera). Two new variants of the K haplotype and two of the J haplotype were found on Western honeybees in what appeared to be well-established infestations. New haplotypes may represent a potential threat to A. mellifera worldwide. The extreme lack of polymorphism in the K and J haplotypes outside of Asia, can now be plausibly explained as being due to genetic 'bottlenecks' that occurred in Asia before and after mites shifted from their original Eastern honeybee host. C1 [Navajas, Maria; Clement, Jeremy] UMR CBGP INRA IRD Cirad Montpellier SupAgro, F-34988 Montferrier Sur Lez, France. [Anderson, Denis L.] CSIRO Entomol, Canberra, ACT 2601, Australia. [de Guzman, Lilia I.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. [Huang, Zachary Y.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Zhou, Ting] Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China. [Le Conte, Yves] UMR INRA UAPV Abeilles & Environm, Lab Biol & Protect Abeille, F-84914 Avignon 9, France. RP Navajas, M (reprint author), UMR CBGP INRA IRD Cirad Montpellier SupAgro, Campus Int Baillarguet,CS 30016, F-34988 Montferrier Sur Lez, France. EM navajas@supagro.inra.fr RI Anderson, Denis/C-3203-2009; Huang, Zachary/D-5485-2011; Le Conte, Yves/B-3246-2016 OI Huang, Zachary/0000-0001-5354-5547; FU Fonds Europeen d'Orientation et de Garantie Agricole [ID B24000059]; USDA-SCP FX We thank Prof. Z. Zeng and Prof. Wongsiri for providing sampling facilities in respectively China, and Thailand and S. Cros-Arteil and C. Moreau for help in sequencing. A part of the sequences in this work are from the graduate diploma, University Montpellier II, defended by J. Clement in 2004. Funding was provided by the European grant FEOGA (Fonds Europeen d'Orientation et de Garantie Agricole) ID B24000059 to MN and YLC, and a USDA-SCP grant to ZYH. Data used in this work were (partly) produced through molecular genetic analysis technical facilities of the IFR119 "Montpellier Environnement Biodiversite". NR 32 TC 23 Z9 23 U1 4 U2 26 PU EDP SCIENCES S A PI LES ULIS CEDEX A PA 17, AVE DU HOGGAR, PA COURTABOEUF, BP 112, F-91944 LES ULIS CEDEX A, FRANCE SN 0044-8435 J9 APIDOLOGIE JI Apidologie PD MAR-APR PY 2010 VL 41 IS 2 BP 181 EP 193 DI 10.1051/apido/2009068 PG 13 WC Entomology SC Entomology GA 580PK UT WOS:000276461200009 ER PT J AU Connor, N Sikorski, J Rooney, AP Kopac, S Koeppel, AF Burger, A Cole, SG Perry, EB Krizanc, D Field, NC Slaton, M Cohan, FM AF Connor, Nora Sikorski, Johannes Rooney, Alejandro P. Kopac, Sarah Koeppel, Alexander F. Burger, Andrew Cole, Scott G. Perry, Elizabeth B. Krizanc, Danny Field, Nicholas C. Slaton, Michele Cohan, Frederick M. TI Ecology of Speciation in the Genus Bacillus SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BACTERIAL DIVERSITY; SP-NOV.; GENETIC EXCHANGE; SPECIES CONCEPT; MICROBIAL DIVERSITY; PERIODIC SELECTION; FUNDAMENTAL UNITS; EVOLUTION-CANYON; SOUTHERN SPAIN; SANTA-ROSALIA AB Microbial ecologists and systematists are challenged to discover the early ecological changes that drive the splitting of one bacterial population into two ecologically distinct populations. We have aimed to identify newly divergent lineages ("ecotypes") bearing the dynamic properties attributed to species, with the rationale that discovering their ecological differences would reveal the ecological dimensions of speciation. To this end, we have sampled bacteria from the Bacillus subtilis-Bacillus licheniformis clade from sites differing in solar exposure and soil texture within a Death Valley canyon. Within this clade, we hypothesized ecotype demarcations based on DNA sequence diversity, through analysis of the clade's evolutionary history by Ecotype Simulation (ES) and AdaptML. Ecotypes so demarcated were found to be significantly different in their associations with solar exposure and soil texture, suggesting that these and covarying environmental parameters are among the dimensions of ecological divergence for newly divergent Bacillus ecotypes. Fatty acid composition appeared to contribute to ecotype differences in temperature adaptation, since those ecotypes with more warm-adapting fatty acids were isolated more frequently from sites with greater solar exposure. The recognized species and subspecies of the B. subtilis-B. licheniformis clade were found to be nearly identical to the ecotypes demarcated by ES, with a few exceptions where a recognized taxon is split at most into three putative ecotypes. Nevertheless, the taxa recognized do not appear to encompass the full ecological diversity of the B. subtilis-B. licheniformis clade: ES and AdaptML identified several newly discovered clades as ecotypes that are distinct from any recognized taxon. C1 [Connor, Nora; Kopac, Sarah; Koeppel, Alexander F.; Burger, Andrew; Cole, Scott G.; Perry, Elizabeth B.; Field, Nicholas C.; Cohan, Frederick M.] Wesleyan Univ, Dept Biol, Middletown, CT 06459 USA. [Krizanc, Danny] Wesleyan Univ, Dept Math & Comp Sci, Middletown, CT 06459 USA. [Sikorski, Johannes] Deutsch Sammlung Mikroorganismen & Zellkulturen G, D-38124 Braunschweig, Germany. [Rooney, Alejandro P.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Slaton, Michele] Death Valley Natl Pk, Death Valley, CA 92328 USA. RP Cohan, FM (reprint author), Wesleyan Univ, Dept Biol, Middletown, CT 06459 USA. EM fcohan@wesleyan.edu OI Kopac, Sarah/0000-0003-1930-535X FU DFG [SI 1352/1-2]; NSF FIBR [EF-0328698]; Wesleyan University FX We are grateful to the National Park Service for supporting our research in Death Valley. This research was supported by funds awarded to J. S. from a DFG grant (SI 1352/1-2) and to F. M. C. from an NSF FIBR grant (EF-0328698), as well as by research funds from Wesleyan University. NR 63 TC 39 Z9 41 U1 1 U2 30 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 5 BP 1349 EP 1358 DI 10.1128/AEM.01988-09 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 559VJ UT WOS:000274855800006 PM 20048064 ER PT J AU Shanks, OC White, K Kelty, CA Hayes, S Sivaganesan, M Jenkins, M Varma, M Haugland, RA AF Shanks, Orin C. White, Karen Kelty, Catherine A. Hayes, Sam Sivaganesan, Mano Jenkins, Michael Varma, Manju Haugland, Richard A. TI Performance Assessment PCR-Based Assays Targeting Bacteroidales Genetic Markers of Bovine Fecal Pollution SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID 16S RIBOSOMAL-RNA; SOURCE IDENTIFICATION; QUANTITATIVE PCR; SOURCE TRACKING; WATER; DNA; SAMPLES; CONTAMINATION; INDICATORS; PATHOGENS AB There are numerous PCR-based assays available to characterize bovine fecal pollution in ambient waters. The determination of which approaches are most suitable for field applications can be difficult because each assay targets a different gene, in many cases from different microorganisms, leading to variation in assay performance. We describe a performance evaluation of seven end-point PCR and real-time quantitative PCR (qPCR) assays reported to be associated with either ruminant or bovine feces. Each assay was tested against a reference collection of DNA extracts from 247 individual bovine fecal samples representing 11 different populations and 175 fecal DNA extracts from 24 different animal species. Bovine-associated genetic markers were broadly distributed among individual bovine samples ranging from 39 to 93%. Specificity levels of the assays spanned 47.4% to 100%. End-point PCR sensitivity also varied between assays and among different bovine populations. For qPCR assays, the abundance of each host-associated genetic marker was measured within each bovine population and compared to results of a qPCR assay targeting 16S rRNA gene sequences from Bacteroidales. Experiments indicate large discrepancies in the performance of bovine-associated assays across different bovine populations. Variability in assay performance between host populations suggests that the use of bovine microbial source-tracking applications will require a priori characterization at each watershed of interest. C1 [Shanks, Orin C.; White, Karen; Kelty, Catherine A.; Hayes, Sam; Sivaganesan, Mano] US EPA, Off Res & Dev, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA. [Jenkins, Michael] USDA ARS, Watkinsville, GA 30677 USA. [Varma, Manju; Haugland, Richard A.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. RP Shanks, OC (reprint author), US EPA, Off Res & Dev, Natl Risk Management Res Lab, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. EM shanks.orin@epa.gov FU U.S. Environmental Protection Agency through its Office of Research and Development FX The U.S. Environmental Protection Agency, through its Office of Research and Development, funded and managed, or partially funded and collaborated in, the research described herein. NR 29 TC 43 Z9 45 U1 2 U2 22 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 5 BP 1359 EP 1366 DI 10.1128/AEM.02033-09 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 559VJ UT WOS:000274855800007 PM 20061457 ER PT J AU Kyle, JL Parker, CT Goudeau, D Brandl, MT AF Kyle, Jennifer L. Parker, Craig T. Goudeau, Danielle Brandl, Maria T. TI Transcriptome Analysis of Escherichia coli O157:H7 Exposed to Lysates of Lettuce Leaves SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID VIRULENCE GENE-EXPRESSION; SALMONELLA-ENTERICA; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; NITRIC-OXIDE; POLYNUCLEOTIDE PHOSPHORYLASE; ANTIBIOTIC-RESISTANCE; ARABIDOPSIS-THALIANA; CHROMOSOMAL GENES; O157-H7 AB Harvesting and processing of leafy greens inherently cause plant tissue damage, creating niches on leaves that human pathogens can exploit. We previously demonstrated that Escherichia coli O157:H7 (EcO157) multiplies more rapidly on shredded leaves than on intact leaves (M. T. Brandl, Appl. Environ. Microbiol. 74:5285-5289, 2008). To investigate how EcO157 cells adapt to physicochemical conditions in injured lettuce tissue, we used microarray-based whole-genome transcriptional profiling to characterize gene expression patterns in EcO157 after 15- and 30-min exposures to romaine lettuce lysates. Multiple carbohydrate transport systems that have a role in the utilization of substrates known to be prevalent in plant cells were activated in EcO157. This indicates the availability to the human pathogen of a variety of carbohydrates released from injured plant cells that may promote its extensive growth in leaf lysates and, thus, in wounded leaf tissue. In addition, microarray analysis revealed the upregulation of numerous genes associated with EcO157 attachment and virulence, with oxidative stress and antimicrobial resistance (including the OxyR and Mar regulons), with detoxification of noxious compounds, and with DNA repair. Upregulation of oxidative stress and antimicrobial resistance genes in EcO157 was confirmed on shredded lettuce by quantitative reverse transcription-PCR. We further demonstrate that this adaptation to stress conditions imparts the pathogen with increased resistance to hydrogen peroxide and calcium hypochlorite. This enhanced resistance to chlorinated sanitizers combined with increased expression of virulence determinants and multiplication at sites of injury on the leaves may help explain the association of processed leafy greens with outbreaks of EcO157. C1 [Kyle, Jennifer L.; Parker, Craig T.; Goudeau, Danielle; Brandl, Maria T.] USDA, ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. RP Brandl, MT (reprint author), USDA, ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. EM maria.brandl@ars.usda.gov FU USDA/ARS; USDA CRIS [5325-42000-044-00D, 5325-42000-045-00D] FX This study was supported by a USDA/ARS award to M. T. B. and by funds from USDA CRIS projects 5325-42000-044-00D and 5325-42000-045-00D. NR 76 TC 57 Z9 57 U1 0 U2 18 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 5 BP 1375 EP 1387 DI 10.1128/AEM.02461-09 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 559VJ UT WOS:000274855800009 PM 20061451 ER PT J AU Pan, Y Breidt, F Gorski, L AF Pan, Youwen Breidt, Frederick, Jr. Gorski, Lisa TI Synergistic Effects of Sodium Chloride, Glucose, and Temperature on Biofilm Formation by Listeria monocytogenes Serotype 1/2a and 4b Strains SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID TURKEY-PROCESSING PLANTS; GROWTH-CONDITIONS; UNITED-STATES; FOOD; ENVIRONMENT; HYDROPHOBICITY; ATTACHMENT; RESISTANCE; ADHERENCE; PATHOGEN AB Biofilm formation by Listeria monocytogenes is generally associated with its persistence in the food-processing environment. Serotype 1/2a strains make up more than 50% of the total isolates recovered from food and the environment, while serotype 4b strains are most often associated with major outbreaks of human listeriosis. Using a microplate assay with crystal violet staining, we examined biofilm formation by 18 strains of each serotype in tryptic soy broth with varying concentrations of glucose (from 0.25% to 10.0%, wt/vol), sodium chloride (from 0.5% to 7.0%, wt/vol) and ethanol (from 1% to 5.0%, vol/vol), and at different temperatures (22.5 degrees C, 30 degrees C, and 37 degrees C). A synergistic effect on biofilm formation was observed for glucose, sodium chloride, and temperature. The serotype 1/2a strains generally formed higher-density biofilms than the 4b strains under most conditions tested. Interestingly, most serotype 4b strains had a higher growth rate than the 1/2a strains, suggesting that the growth rate may not be directly related to the capacity for biofilm formation. Crystal violet was found to stain both bacterial cells and biofilm matrix material. The enhancement in biofilm formation by environmental factors was apparently due to the production of extracellular polymeric substances instead of the accumulation of viable biofilm cells. C1 [Breidt, Frederick, Jr.] N Carolina State Univ, USDA ARS, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Pan, Youwen] N Carolina State Univ, Dept Microbiol, Raleigh, NC 27695 USA. [Gorski, Lisa] USDA ARS, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. RP Breidt, F (reprint author), N Carolina State Univ, USDA ARS, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. EM fred.breidt@ars.usda.gov FU Pickle Packers Intl., Inc., Washington, DC FX This investigation was supported in part by a research grant from Pickle Packers Intl., Inc., Washington, DC. NR 31 TC 36 Z9 38 U1 0 U2 18 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 5 BP 1433 EP 1441 DI 10.1128/AEM.02185-09 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 559VJ UT WOS:000274855800016 PM 20048067 ER PT J AU Lang, P Lefebure, T Wang, W Bitar, PP Meinersmann, RJ Kaya, K Stanhope, MJ AF Lang, Ping Lefebure, Tristan Wang, Wei Bitar, Paulina Pavinski Meinersmann, Richard J. Kaya, Katherine Stanhope, Michael J. TI Expanded Multilocus Sequence Typing and Comparative Genomic Hybridization of Campylobacter coli Isolates from Multiple Hosts SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID GUILLAIN-BARRE-SYNDROME; POPULATION-STRUCTURE; GENOTYPE DATA; GENE-EXPRESSION; DNA MICROARRAY; PAN-GENOME; JEJUNI; STRAINS; EVOLUTION; INFERENCE AB The purpose of this work was to evaluate the evolutionary history of Campylobacter coli isolates derived from multiple host sources and to use microarray comparative genomic hybridization to assess whether there are particular genes comprising the dispensable portion of the genome that are more commonly associated with certain host species. Genotyping and ClonalFrame analyses of an expanded 16-gene multilocus sequence typing (MLST) data set involving 85 isolates from 4 different hosts species tentatively supported the development of C. coli host-preferred groups and suggested that recombination has played various roles in their diversification; however, geography could not be excluded as a contributing factor underlying the history of some of the groups. Population genetic analyses of the C. coli pubMLST database by use of STRUCTURE suggested that isolates from swine form a relatively homogeneous genetic group, that chicken and human isolates show considerable genetic overlap, that isolates from ducks and wild birds have similarity with environmental water samples and that turkey isolates have a connection with human infection similar to that observed for chickens. Analysis of molecular variance (AMOVA) was performed on these same data and suggested that host species was a significant factor in explaining genetic variation and that macrogeography (North America, Europe, and the United Kingdom) was not. The microarray comparative genomic hybridization data suggested that there were combinations of genes more commonly associated with isolates derived from particular hosts and, combined with the results on evolutionary history, suggest that this is due to a combination of common ancestry in some cases and lateral gene transfer in others. C1 [Lang, Ping; Lefebure, Tristan; Bitar, Paulina Pavinski; Stanhope, Michael J.] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. [Wang, Wei] Cornell Univ, Life Sci Core Labs Ctr, Ithaca, NY 14853 USA. [Meinersmann, Richard J.] USDA ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30604 USA. [Kaya, Katherine] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP Stanhope, MJ (reprint author), Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. EM mjs297@cornell.edu RI Lefebure, Tristan/A-6489-2009 OI Lefebure, Tristan/0000-0003-3923-8166 FU NIH [N01-AI-30054 (ZC003-05)] FX This work was supported by NIH contract N01-AI-30054 (ZC003-05), awarded to M.J.S. NR 62 TC 18 Z9 18 U1 4 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 6 BP 1913 EP 1925 DI 10.1128/AEM.01753-09 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 564DQ UT WOS:000275193900024 PM 20097815 ER PT J AU Jenkins, MB Eaglesham, BS Anthony, LC Kachlany, SC Bowman, DD Ghiorse, WC AF Jenkins, Michael B. Eaglesham, Barbara S. Anthony, Larry C. Kachlany, Scott C. Bowman, Dwight D. Ghiorse, William C. TI Significance of Wall Structure, Macromolecular Composition, and Surface Polymers to the Survival and Transport of Cryptosporidium parvum Oocysts SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CELL-WALL; INACTIVATION; TEMPERATURE; WATER; SOIL; INFECTIVITY; GENOME; FECES AB The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and hydrologic transport of oocysts in terrestrial and aquatic environments. Results of microscopy showed an outer electron-dense layer, a translucent middle layer, two inner electron-dense layers, and a suture structure embedded in the inner electron-dense layers. Freeze-substitution showed an expanded glycocalyx layer external to the outer bilayer, and Alcian Blue staining confirmed its presence on some but not all oocysts. Biochemical analyses of purified oocyst walls revealed carbohydrate components, medium-and long-chain fatty acids, and aliphatic hydrocarbons. Purified walls contained 7.5% total protein (by the Lowry assay), with five major bands in SDS-PAGE gels. Staining of purified oocyst walls with magnesium anilinonaphthalene-8-sulfonic acid indicated the presence of hydrophobic proteins. These structural and biochemical analyses support a model of the oocyst wall that is variably impermeable and resistant to many environmental pressures. The strength and flexibility of oocyst walls appear to depend on an inner layer of glycoprotein. The temperature-dependent permeability of oocyst walls may be associated with waxy hydrocarbons in the electron-translucent layer. The complex chemistry of these layers may explain the known acid-fast staining properties of oocysts, as well as some of the survival characteristics of oocysts in terrestrial and aquatic environments. The outer glycocalyx surface layer provides immunogenicity and attachment possibilities, and its ephemeral nature may explain the variable surface properties noted in oocyst hydrologic transport studies. C1 [Jenkins, Michael B.; Eaglesham, Barbara S.; Anthony, Larry C.; Kachlany, Scott C.; Ghiorse, William C.] Cornell Univ, Dept Microbiol, Coll Agr & Life Sci, Ithaca, NY 14853 USA. [Bowman, Dwight D.] Cornell Univ, Dept Microbiol & Immunol, Coll Vet Med, Ithaca, NY 14853 USA. RP Jenkins, MB (reprint author), USDA ARS, 1420 Expt Stn Rd, Watkinsville, GA 30677 USA. EM michael.jenkins@ars.usda.gov FU USDA; Cornell University College of Agriculture and Life Sciences FX This study was supported in part by the USDA and the Cornell University College of Agriculture and Life Sciences. NR 48 TC 16 Z9 16 U1 2 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 6 BP 1926 EP 1934 DI 10.1128/AEM.02295-09 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 564DQ UT WOS:000275193900025 PM 20097810 ER PT J AU Lu, Z Altermann, E Breidt, F Kozyavkin, S AF Lu, Z. Altermann, E. Breidt, F. Kozyavkin, S. TI Sequence Analysis of Leuconostoc mesenteroides Bacteriophage Phi 1-A4 Isolated from an Industrial Vegetable Fermentation SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID COMPLETE GENOME SEQUENCE; LACTOBACILLUS-PLANTARUM BACTERIOPHAGE; TRANSCRIPTIONAL REPRESSOR COPG; GROUP-II INTRONS; STAPHYLOCOCCUS-EPIDERMIDIS; ESCHERICHIA-COLI; LACTOCOCCAL BACTERIOPHAGES; MOLECULAR CHARACTERIZATION; SAUERKRAUT FERMENTATION; FERMENTING SAUERKRAUT AB Vegetable fermentations rely on the proper succession of a variety of lactic acid bacteria (LAB). Leuconostoc mesenteroides initiates fermentation. As fermentation proceeds, L. mesenteroides dies off and other LAB complete the fermentation. Phages infecting L. mesenteroides may significantly influence the die-off of L. mesenteroides. However, no L. mesenteroides phages have been previously genetically characterized. Knowledge of more phage genome sequences may provide new insights into phage genomics, phage evolution, and phage-host interactions. We have determined the complete genome sequence of L. mesenteroides phage Phi 1-A4, isolated from an industrial sauerkraut fermentation. The phage possesses a linear, double-stranded DNA genome consisting of 29,508 bp with a G + C content of 36%. Fifty open reading frames (ORFs) were predicted. Putative functions were assigned to 26 ORFs (52%), including 5 ORFs of structural proteins. The phage genome was modularly organized, containing DNA replication, DNA-packaging, head and tail morphogenesis, cell lysis, and DNA regulation/modification modules. In silico analyses showed that Phi 1-A4 is a unique lytic phage with a large-scale genome inversion (similar to 30% of the genome). The genome inversion encompassed the lysis module, part of the structural protein module, and a cos site. The endolysin gene was flanked by two holin genes. The tail morphogenesis module was interspersed with cell lysis genes and other genes with unknown functions. The predicted amino acid sequences of the phage proteins showed little similarity to other phages, but functional analyses showed that Phi 1-A4 clusters with several Lactococcus phages. To our knowledge, Phi 1-A4 is the first genetically characterized L. mesenteroides phage. C1 [Lu, Z.] Kennesaw State Univ, Kennesaw, GA 30144 USA. [Lu, Z.] AgResearch Ltd, Grasslands Res Ctr, Palmerston North, New Zealand. [Breidt, F.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Breidt, F.] ARS, USDA, Raleigh, NC 27695 USA. [Kozyavkin, S.] Fidel Syst Inc, Gaithersburg, MD USA. RP Lu, Z (reprint author), Kennesaw State Univ, 1000 Chastain Rd, Kennesaw, GA 30144 USA. EM jean_lu@kennesaw.edu FU Kennesaw State University; Pickle Packers International Inc., Washington, DC FX This study was partially supported by the Kennesaw State University Incentive Fund and by Pickle Packers International Inc., Washington, DC. NR 77 TC 23 Z9 23 U1 0 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 6 BP 1955 EP 1966 DI 10.1128/AEM.02126-09 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 564DQ UT WOS:000275193900028 PM 20118355 ER PT J AU Schlatter, DC Samac, DA Tesfaye, M Kinkel, LL AF Schlatter, Daniel C. Samac, Deborah A. Tesfaye, Mesfin Kinkel, Linda L. TI Rapid and Specific Method for Evaluating Streptomyces Competitive Dynamics in Complex Soil Communities SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RIBOSOMAL-RNA GENE; GENOME SEQUENCE; PATHOGENIC STREPTOMYCES; COELICOLOR A3(2); GRISEUS AB Quantifying target microbial populations in complex communities remains a barrier to studying species interactions in soil environments. Quantitative PCR (qPCR) assays were developed for quantifying pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae strains in complex soil communities. This assay will be useful for evaluating the competitive dynamics of streptomycetes in soil. C1 [Schlatter, Daniel C.; Samac, Deborah A.; Tesfaye, Mesfin; Kinkel, Linda L.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Samac, Deborah A.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. RP Kinkel, LL (reprint author), 1991 Upper Buford Circle,495 Borlaug Hall, St Paul, MN 55108 USA. EM kinkel@umn.edu FU National Science Foundation [DEB-0543213]; USDA [2006-35319-17445] FX This work was supported by award DEB-0543213 from the National Science Foundation and by USDA grant 2006-35319-17445. NR 11 TC 10 Z9 10 U1 1 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2010 VL 76 IS 6 BP 2009 EP 2012 DI 10.1128/AEM.02320-09 PG 4 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 564DQ UT WOS:000275193900035 PM 20080993 ER PT J AU Byler, RK Jordan, AG AF Byler, R. K. Jordan, A. G. TI MODEL OF COTTON BALE WEIGHT CHANGES RELATED TO BAGGING SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Cotton; Cotton bale; Bale bagging; Moisture content; Ginning AB Traditionally, bagging used for cotton bales has been permeable to moisture, but more recently relatively impermeable material has been adopted by the industry. This impermeable bagging has significantly affected the moisture changes that occur during storage. In many cases holes have been punched in the bagging in an effort to increase the permeability. To better understand the effect of different bagging materials on bale weight changes, Universal Density cotton bales were formed with relatively dry lint and covered with different types of bale bagging materials. The bales were stored in a humid environment and weighed periodically for between 63 days, for high permeability bagging, to 155 days, for low permeability bagging. The bale coverings studied included coated woven polypropylene with micro-perf holes, linear low density polyethylene film with different hole patterns, woven cotton, woven burlap, and no bale bagging. The bale weights were modeled temporally using a nonlinear exponential model. Different bales stored in the same bagging type changed weight consistently. A mathematical model was developed for polyethylene bagging that accurately related the rate of bale weight change with the percentage of bag surface removed as holes. The rate of bale weight change obtained from the regression was used to calculate a half-time to equilibrium, in days, for each bagging type. This weight change half-time will simplify communication of the effect bale bagging has on cotton bale weight change during storage. C1 [Byler, R. K.] ARS, USDA, Cotton Ginning Res Unit, Stoneville, MS 38776 USA. [Jordan, A. G.] Natl Cotton Council, Memphis, TN USA. RP Byler, RK (reprint author), ARS, USDA, Cotton Ginning Res Unit, 111 Expt Stn Rd,POB 256, Stoneville, MS 38776 USA. EM rick.byler@ars.usda.gov NR 11 TC 0 Z9 1 U1 0 U2 0 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 203 EP 208 PG 6 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700003 ER PT J AU Holt, GA Laird, JW AF Holt, G. A. Laird, J. W. TI POWER ROLL GIN STAND TECHNOLOGY: EVALUATION AND OPTIMIZATION OF RIB RAIL ANGLE AND GINNING POINT MODIFICATIONS ON A RETROFITTED LUMMUS GIN STAND SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Cotton gin; Cotton; Gin stand; Fiber quality; Seed cotton; Gin; Ginning; Ginning rate; Turnout ID DESIGN AB Previous studies have shown the powered roll gin stand (PRGS) is capable of improving ginning rate, turnout, and fiber quality relative to a conventional gin stand; however; most of these studies used Continental Eagle gin stands, the gin stand used to develop the initial prototype and the one used in the initial field trials. Field experience in commercial gins where the PRGS technology was installed on non-Continental Eagle equipment did not always show the levels of improvement previously reported. Investigation as to the differences between the models of gin stands for which the powered roll was working and those for which it had less than desirable results revealed the primary differences were the rib rail angle and location of the ginning point. A study was conducted on a retrofitted Lummus-116 gin stand to evaluate the hypothesis that the rib rail angle and ginning point location were the reasons why the PRGS technology was not as effective on makes of gin stands other than those used to develop the technology. Results validated the hypothesis and indicated that the rib rail angle needed to be reduced by 4 and the gin point increased by 12.7 mm from the initial settings on the Lummus-116. The findings demonstrate that the initial concept of all that was needed to implement the PRGS technology on an existing gin stand was to replace the gin front was in error: the rib rail angle and ginning point location need to be considered when retrofitting any existing gin stand with PRGS technology. C1 [Holt, G. A.; Laird, J. W.] ARS, USDA, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. RP Holt, GA (reprint author), ARS, USDA, Cotton Prod & Proc Res Unit, 1604 E FM 1294, Lubbock, TX 79403 USA. EM greg.holt@ars.usda.gov FU Cotton Incorporated; PRT Marketing LLC FX The partial support of this research by Cotton Incorporated and PRT Marketing LLC is gratefully acknowledged. NR 18 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 209 EP 215 PG 7 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700004 ER PT J AU Norton, LD Savabi, R AF Norton, L. D. Savabi, R. TI EVOLUTION OF A LINEAR VARIABLE INTENSITY RAINFALL SIMULATOR FOR SURFACE HYDROLOGY AND EROSION STUDIES SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Soil erosion; Infiltration; Runoff; Soil amendments; Water quality ID SILT LOAM SOIL; WATER CHEMISTRY; AMENDMENTS; RUNOFF AB Because of wind erosion during the dust bowl era of the early 1930s in the central United States, Congress appropriated federal funding to study the problem and find ways that protect the land from all forms of erosion. Afterwards, a series of erosion stations were established across the eastern United States, and natural rainfall-runoff plots were placed on a wide range of soils, slopes, and cropping/management systems. The data collected from these studies served as the basis for developing the Universal Soil Loss Equation (USLE) for rain-induced erosion which is still, in its revised form, the basis for determining "highly erodible lands" for various USDA programs. Because of the expense of maintaining USLE plots, and the extreme variability in. the research data, they were discontinued, and most research since the 1950s in water erosion has focused on using rainfall simulators to quantify differences in erosion from all the variables of the USLE, and refining other parameters, such as factors K and C. The simulators used over the last 60 years have varied widely in design and functionality, but paid little or no attention to the type of water used to conduct the simulation. The objective of this article is to describe relevant advances in the historical development of simulator technology in the United States and describe principal design improvements that allow them to be used in a wider range of rainfall-runoff and erosion studies. This article will also describe the most recent portable simulator developed at the National Soil Erosion Research Laboratory. C1 [Norton, L. D.] Purdue Univ, USDA, ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Savabi, R.] ARS, USDA, Subtrop Hort Res Stn, Miami, FL USA. RP Norton, LD (reprint author), Purdue Univ, USDA, ARS, Natl Soil Eros Res Lab, 275 S Russell St, W Lafayette, IN 47907 USA. EM norton@purdue.edu NR 25 TC 3 Z9 3 U1 0 U2 2 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 239 EP 245 PG 7 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700008 ER PT J AU Haberland, JA Colaizzi, PD Kostrzewski, MA Waller, PM Choi, CY Eaton, EE Barnes, EM Clarke, TR AF Haberland, J. A. Colaizzi, P. D. Kostrzewski, M. A. Waller, P. M. Choi, C. Y. Eaton, E. E. Barnes, E. M. Clarke, T. R. TI AGIIS, AGRICULTURAL IRRIGATION IMAGING SYSTEM SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Remote sensing; Irrigation; Center pivot; Linear move; Ndvi; Cwsi; Ccci ID NITROGEN STATUS; WATER-STRESS; VEGETATION AB AgIIS (Agricultural Irrigation Imaging System, pronounced Ag Eyes), a ground-based remote sensing system, served as a research tool that generated data for research on remotely sensed canopy level water and nitrogen status indices. A rail was mounted on a 100-m long linear move irrigation machine, and a cart with a remote sensing unit ran back and forth on the rail. As the cart traveled along the rail and the linear move traveled through the field, the sensing unit collected one square meter area reflectance measurements every meter along the rail. Because the system was automated, the remotely sensed data was acquired with low labor cost compared to traditional handheld radiometers, and provided high temporal and spatial resolution. The system monitored a 0.5-ha research area with 16 research plots. The rail, made of steel tubing, was constructed of three parallel tubes in a triangular frame. The rail had almost no vertical deflection due to cart weight, and slip joints between sections were elastic enough to absorb the deformation of the linear move when loaded with water. The sensor package included four reflectance bands filtered to narrow wavelength intervals (10 nm) in the red (670 nm), green (555 nm), red-edge (720 tun), and near infrared (NIR) (790 mini) portions of the spectrum, and an infrared thermometer. The crop spectral signals were post-processed in order to construct georeferenced field maps of vegetation, nutrient, and water status indices. Analysis of the data showed that the rail and cart provided a platform for collection of consistent and reliable remote sensing data, and it served as a valuable tool for refinement of water and nitrogen status indices. The AgIIS design effectively and reliably collected remote sensing data from a constant elevation, at near nadir orientation, and at 1-m intervals. C1 [Waller, P. M.; Choi, C. Y.] Univ Arizona, Dept Agr & Biosyst Engn, Tucson, AZ 85721 USA. [Haberland, J. A.] Univ Chile, Dept Ingn & Suelos, Fac Ciencias Agron, Santiago, Chile. [Colaizzi, P. D.] ARS, USDA, Conservat & Prod Res Lab, Bushland, TX USA. [Kostrzewski, M. A.] Pima Cty Wastewater Treatment Dept, Tucson, AZ USA. [Eaton, E. E.] New Mexico State Univ, Extens Serv, Las Cruces, NM 88003 USA. [Barnes, E. M.] Cotton Inc, Cary, NC USA. [Clarke, T. R.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. RP Waller, PM (reprint author), Univ Arizona, Dept Agr & Biosyst Engn, 403 Shantz Bldg, Tucson, AZ 85721 USA. EM pwaller@ag.arizona.edu OI Waller, Peter/0000-0002-1696-3800 FU US-DOE Idaho National Environmental and Engineering Laboratory FX Primary funding for this effort was from the US-DOE Idaho National Environmental and Engineering Laboratory. NR 14 TC 5 Z9 5 U1 1 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 247 EP 253 PG 7 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700009 ER PT J AU King, BA Winward, TW Bjorneberg, DL AF King, B. A. Winward, T. W. Bjorneberg, D. L. TI LASER PRECIPITATION MONITOR FOR MEASUREMENT OF DROP SIZE AND VELOCITY OF MOVING SPRAY-PLATE SPRINKLERS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Sprinkler; Moving spray-plate; Drop size; Drop velocity; Kinetic energy; Center pivot ID OPTICAL SPECTRO PLUVIOMETER; KINETIC-ENERGY; IRRIGATION SPRINKLERS; SIMULATION-MODEL; DISTRIBUTIONS; NOZZLES; WIND; SPECTROPLUVIOMETER; INFILTRATION; PERFORMANCE AB Sprinkler drop size distribution and associated drop velocities have a major influence on sprinkler performance in regards to kinetic energy transferred to the soil surface. A laser instrument commercially marketed for real-time rainfall measurements as a Laser Precipitation Monitor (LPM) was used to measure drop size and velocity from 10 moving spray-plate type sprinklers. Measured drop size and velocity were used to determine sprinkler drop size distribution and kinetic energy applied to the soil by sprinkler discharge. Drop size distributions measured by the LPM were compared to drop size distributions measured in earlier studies using the traditional flour pellet method. Eight of the ten measured drop size distributions were not significantly different between measurement methods. However; the operating conditions when the two methods did not compare well were outside sprinkler manufacturer specifications. Based on this limited study, the results from the two drop size measurement methods can be vastly different for sprinklers under operating conditions that produce relatively compact streams of water drops; which method is more accurate for this condition remains unknown. Kinetic energy values calculated using measured drop size and velocity data were not significantly different from values determined using flour pellet drop size data and a ballistic model for estimating sprinkler droplet tangential velocity. The laser instrument used in this study provided a relatively easy means to obtain reliable estimates of sprinkler kinetic energy per unit volume of applied water for various moving spray-plate sprinkler types and operating conditions. Measured drop size distribution and computed kinetic energy applied by sprinkler discharge is sufficient for practical field application purposes. C1 [King, B. A.; Winward, T. W.; Bjorneberg, D. L.] ARS, USDA, NWISRL, Kimberly, ID 83341 USA. RP King, BA (reprint author), ARS, USDA, NWISRL, 3793 N 3600 E, Kimberly, ID 83341 USA. EM brad.king@ars.usda.gov NR 36 TC 9 Z9 16 U1 0 U2 7 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 263 EP 271 PG 9 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700011 ER PT J AU O'Shaughnessy, SA Evett, SR AF O'Shaughnessy, S. A. Evett, S. R. TI DEVELOPING WIRELESS SENSOR NETWORKS FOR MONITORING CROP CANOPY TEMPERATURE USING A MOVING SPRINKLER SYSTEM AS A PLATFORM SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Crop canopy temperature; Infrared thermometry; Wireless sensors; Sensor networks ID WATER-STRESS INDEX; INFRARED THERMOMETERS; IRRIGATION MANAGEMENT; COTTON; CORN; YIELD; EVAPOTRANSPIRATION AB The objectives of this study were to characterize wireless sensor nodes that we developed in terms of power consumption and functionality, and compare the performance of mesh and non-mesh wireless sensor networks (WSNs) comprised mainly of infrared thermometer thermocouples located on a center pivot lateral and in the field below The sensor nodes mounted on masts fixed to the lateral arm of a center pivot irrigation system functioned to monitor crop canopy temperatures while the system moved,. the sensor nodes established in the field below the pivot were to provide stationary reference canopy temperatures. The WSNs located in cropped fields independent of the irrigation system functioned in a highly reliable manner [packet reception percentage (PRP) > 94]. Mesh-networking was the single communication protocol that provided functionality for the WSN located on the center pivot lateral. Its PRP was 84 and 87 during the 2007 and 2008 growing seasons, respectively. Future research is required for thorough testing and optimizing of.WSNs for automatic control and irrigation scheduling of a center pivot system. C1 [O'Shaughnessy, S. A.; Evett, S. R.] ARS, USDA, CPRL, Bushland, TX 79012 USA. RP O'Shaughnessy, SA (reprint author), ARS, USDA, CPRL, PO Drawer 10, Bushland, TX 79012 USA. EM Sussan.OShaughnessy@ars.usda.gov FU Ogallala Aquifer Program; Bilateral Agricultural Research and Development (BARD); Texas Department of Agriculture [TIE04-01] FX The authors wish to acknowledge funding from the Ogallala Aquifer Program and from a joint grant from the Bilateral Agricultural Research and Development (BARD) fund and Texas Department of Agriculture (grant no. TIE04-01). We also acknowledge with great appreciation the assistance provided by Chad Ford, Agricultural Science Technician, and Brice Ruthardt, Biological Science Technician. NR 52 TC 23 Z9 23 U1 0 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2010 VL 26 IS 2 BP 331 EP 341 PG 11 WC Agricultural Engineering SC Agriculture GA 593WC UT WOS:000277491700017 ER PT J AU Han, SN Pang, E Zingg, JM Meydani, SN Meydani, M Azzi, A AF Han, Sung Nim Pang, Eunice Zingg, Jean-Marc Meydani, Simin Nikbin Meydani, Mohsen Azzi, Angelo TI Differential effects of natural and synthetic vitamin E on gene transcription in murine T lymphocytes SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE RRR-alpha-tocopherol; Gene expression; Synthetic alpha-tocopherol; T-cell ID TRANSFER PROTEIN-DEFICIENCY; ALPHA-TOCOPHEROL; GAMMA-TOCOPHEROL; CELL-PROLIFERATION; NUCLEAR RECEPTOR; OLD MICE; EXPRESSION; AGE; INHIBITION; NETWORKS AB Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differences between the two vitamin forms in regulating gene expression in response to T-cell stimulation. Marker genes have been found whose expression can be considered significant in establishing the level of, and response to vitamin E for both natural and synthetic vitamin E supplementation; unique markers for synthetic vitamin E supplementation and unique markers for natural vitamin E supplementation have been identified. (C) 2009 Elsevier Inc. All rights reserved. C1 [Pang, Eunice; Zingg, Jean-Marc; Meydani, Simin Nikbin; Meydani, Mohsen; Azzi, Angelo] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Han, Sung Nim] Seoul Natl Univ, Dept Food & Nutr, Coll Human Ecol, Seoul, South Korea. RP Azzi, A (reprint author), Tufts Univ, JM USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM angelo.azzi@tufts.edu FU NIDDK NIH HHS [P30 DK040561, P30 DK040561-14] NR 19 TC 12 Z9 14 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD MAR 1 PY 2010 VL 495 IS 1 BP 49 EP 55 DI 10.1016/j.abb.2009.12.015 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 563MQ UT WOS:000275137000008 PM 20026030 ER PT J AU Liu, L Dharne, M Kannan, P Smith, A Meng, JH Fan, MT Boren, TL Ranallo, RT Bhagwat, AA AF Liu, Liu Dharne, Mahesh Kannan, Porteen Smith, Allen Meng, Jianghong Fan, Mingtao Boren, Tara L. Ranallo, Ryan T. Bhagwat, Arvind A. TI Osmoregulated periplasmic glucans synthesis gene family of Shigella flexneri SO ARCHIVES OF MICROBIOLOGY LA English DT Article DE Periplasmic glucans; Low osmolarity; Food- and water-borne Shigellosis ID ENTERICA SEROVAR TYPHIMURIUM; ESCHERICHIA-COLI; BIOSYNTHESIS; OLIGOSACCHARIDES; RESISTANCE; PROTEIN; MICE AB Osmoregulated periplasmic glucans (OPGs) of food- and water-borne enteropathogen Shigella flexneri were characterized. OPGs were composed of 100% glucose with 2-linked glucose as the most abundant residue with terminal glucose, 2-linked and 2,6-linked glucose also present in high quantities. Most dominant backbone polymer chain length was seven glucose residues. Individual genes from the opg gene family comprising of a bicistronic operon opgGH, opgB, opgC and opgD were mutagenized to study their effect on OPGs synthesis, growth in hypo-osmotic media and ability to invade HeLa cells. Mutation in opgG and opgH abolished OPGs biosynthesis, and mutants experienced longer lag time to initiate growth in hypo-osmotic media. Longer lag times to initiate growth in hypo-osmotic media were also observed for opgC and opgD mutants but not for opgB mutant. All opg mutants were able to infect HeLa cells, and abolition of OPGs synthesis did not affect actin polymerization or plaque formation. Ability to synthesize OPGs was beneficial to bacteria in order to initiate growth under low osmolarity conditions, in vitro mammalian cell invasion assays, however, could not discriminate whether OPGs were required for basic aspect of Shigella virulence. C1 [Liu, Liu; Dharne, Mahesh; Kannan, Porteen; Bhagwat, Arvind A.] ARS, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Liu, Liu; Fan, Mingtao] NW A&F Univ, Coll Food Sci & Technol, Yangling 712100, Peoples R China. [Smith, Allen] ARS, Diet Genom & Immunol Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Liu, Liu; Meng, Jianghong] Univ Maryland, Dept Food Sci & Nutr, College Pk, MD 20742 USA. [Boren, Tara L.; Ranallo, Ryan T.] Walter Reed Army Inst Res, Div Bacterial & Rickettsial Dis, Silver Spring, MD USA. RP Bhagwat, AA (reprint author), ARS, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave,Bldg 002,BARC W, Beltsville, MD 20705 USA. EM arvind.bhagwat@ars.usda.gov RI Dharne, Mahesh/K-3541-2012; OI Kannan, Porteen/0000-0002-6925-328X FU China Scholarship Council; Ministry of Education, China FX The study was supported in part by the China Scholarship Council, Ministry of Education, China (LL). We would like to thank Malabi Venkatesan for support of this project. The content of this publication does not necessarily reflect the views or policies of the US Department of the Army, US Department of Agriculture or the US Department of Defense nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 27 TC 3 Z9 3 U1 0 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0302-8933 J9 ARCH MICROBIOL JI Arch. Microbiol. PD MAR PY 2010 VL 192 IS 3 BP 167 EP 174 DI 10.1007/s00203-009-0538-z PG 8 WC Microbiology SC Microbiology GA 554SV UT WOS:000274455600003 PM 20062978 ER PT J AU Dhar, AK Lakshman, DK Amundsen, K Robles-Sikisaka, R Kaizer, KN Roy, S Hasson, KW Allnutt, FCT AF Dhar, Arun K. Lakshman, Dilip K. Amundsen, Keenan Robles-Sikisaka, Refugio Kaizer, Krista N. Roy, Sribash Hasson, Kenneth W. Allnutt, F. C. Thomas TI Characterization of a Taura syndrome virus isolate originating from the 2004 Texas epizootic in cultured shrimp SO ARCHIVES OF VIROLOGY LA English DT Article ID DEPENDENT RNA-POLYMERASE; PENAEUS-VANNAMEI; TSV; AMERICA; HISTOPATHOLOGY; SEQUENCE; PROBES AB A comprehensive investigation of the Taura syndrome virus (TSV) isolate that caused epizootics in shrimp farms in Texas in 2004 (Texas isolate) revealed that this virus was more virulent in laboratory bioassays than the TSV reference isolate, Hawaii 1994, causing severe symptom development and rapid mortality. Histopathology of moribund animals demonstrated epithelial necrosis within the stomach, appendages, general body cuticle and gills, and the surviving animals demonstrated moderate to numerous lymphoid organ spheroids. Purified virions showed icosahedral morphology, with a diameter of 31 nm. Comparative genome analysis showed that the Texas isolate is more closely related to TSV isolates from Thailand and China than to the Hawaii isolate. The predicted tertiary structures of the inhibition of apoptosis protein (IAP) and protease domains of the Texas isolate are very similar to those of the Hawaii isolate. However, the RNA-dependent RNA polymerase (RdRp) of the Texas isolate has significant structural differences from the Hawaii isolate due to point mutation(s) in the RdRp gene. Changes in the RdRp tertiary structure might contribute to the replication fidelity, virulence and ecological adaptability of the Texas isolate. C1 [Hasson, Kenneth W.] Texas Vet Med Diagnost Labs, College Stn, TX 77841 USA. [Robles-Sikisaka, Refugio] Hubbs SeaWorld Res Inst, San Diego, CA 92109 USA. [Lakshman, Dilip K.; Amundsen, Keenan] USDA ARS, SASL, Beltsville, MD 20705 USA. [Dhar, Arun K.; Kaizer, Krista N.; Roy, Sribash; Allnutt, F. C. Thomas] Adv BioNutr Corp, Columbia, MD 21046 USA. RP Dhar, AK (reprint author), Viracine Therapeut Corp, 7155-H Columbia Gateway Dr, Columbia, MD 21046 USA. EM ADhar@abn-corp.com OI Allnutt, F C Thomas/0000-0002-6344-7358 FU Defense Advanced Research Projects Agency (DARPA); Defense Threat Reduction Agency (DTRA) [HDTRA1-07-C-0078] FX This project received support from the Defense Advanced Research Projects Agency (DARPA) and from the Defense Threat Reduction Agency (DTRA) Chemical and Biological Technologies Directorate Transformational Medical Technologies Initiative under DTRA contract HDTRA1-07-C-0078. The authors would like to thank Dr. Narayan Khan, Medicore Inc., Rockville, MD for purifying the virus. NR 36 TC 5 Z9 7 U1 0 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD MAR PY 2010 VL 155 IS 3 BP 315 EP 327 DI 10.1007/s00705-009-0584-5 PG 13 WC Virology SC Virology GA 560XW UT WOS:000274939600003 PM 20049618 ER PT J AU Gulsen, O Eickhoff, T Heng-Moss, T Shearman, R Baxendale, F Sarath, G Lee, D AF Gulsen, Osman Eickhoff, Thomas Heng-Moss, Tiffany Shearman, Robert Baxendale, Frederick Sarath, Gautam Lee, Donald TI Characterization of peroxidase changes in resistant and susceptible warm-season turfgrasses challenged by Blissus occiduus SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Buffalograss; Chinch bug; Zoysiagrass; Oxidative enzymes; Plant resistance ID CHINCH BUG; BUFFALOGRASS GERMPLASM; OXIDATIVE RESPONSES; INSECT HERBIVORY; HEMIPTERA; LYGAEIDAE; LEAVES; POLYMORPHISM; ANTIXENOSIS; EXPRESSION AB Peroxidases play an important role in plant stress related interactions. This research assessed the role of peroxidases in the defense response of resistant and susceptible buffalograsses [Buchloe dactyloides (Nutt.) Engelm] and zoysiagrasses (Zoysia japonica Steudel) to the western chinch bug, Blissus occiduus Barber. The objectives were: (1) to assess the relationships among protein content, basal peroxidase levels, chinch bug injury, and ploidy levels of chinch bug-resistant and -susceptible buffalograsses; (2) to compare peroxidase activity levels of resistant and susceptible buffalograsses and zoysiagrasses in response to chinch bug feeding; (3) and to analyze extracted proteins from chinch bug-resistant and -susceptible buffalograsses and zoysiagrasses by native gel electrophoresis to obtain information on the peroxidase profiles. Correlation analyses of 28 buffalograss genotypes with varying levels of chinch bug resistance and ploidy levels indicated that buffalograss total protein content was correlated (r = 0.47, P = 0.01) to chinch bug injury, while basal peroxidase levels was not (r = 0.19, P = 0.29), suggesting that the up-regulation of peroxidases in resistant buffalograsses is a direct response to chinch bug feeding. Three of the four chinch bug-resistant buffalograss genotypes evaluated had higher peroxidase activity in the infested plants compared to control plants. Peroxidase activity levels were similar between infested and control plants of the two highly susceptible buffalograss genotypes. Zoysiagrasses had lower peroxidase activity in general when compared to buffalograss control plants, and only 'Zorro' consistently showed higher peroxidase activity in the infested plants. Native gel electrophoresis analysis identified differences in the isozyme profiles of infested and control buffalograsses 'Prestige' and 196, and the zoysiagrass 'Zorro'. Results from this study suggest that peroxidases have the potential to be used as markers for selecting chinch bug resistant turfgrasses, and may help explain how plants defend themselves against biotic stresses, such as chinch bugs. C1 [Heng-Moss, Tiffany; Baxendale, Frederick] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Gulsen, Osman] Erciyes Univ, Dept Hort, Kayseri, Turkey. [Eickhoff, Thomas] Monsanto Co, Monmouth, IL 61462 USA. [Shearman, Robert; Lee, Donald] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Sarath, Gautam] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Heng-Moss, T (reprint author), Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. EM thengmoss2@unl.edu RI Heng-Moss, Tiffany/F-8200-2013 FU University of Nebraska Agricultural Experiment Station [NEB-28-097, NEB-22-331, NEB-28-092]; United States Golf Association; Nebraska Turfgrass Association FX We gratefully acknowledge Paul Nabity, Herbert Siqueira, Mitch Stamm, Sandra Schaeffer, and Andrea Gutsche for technical assistance. This research was supported in part by the University of Nebraska Agricultural Experiment Station Projects NEB-28-097, NEB-22-331, AND NEB-28-092, the United States Golf Association, and the Nebraska Turfgrass Association. NR 35 TC 29 Z9 31 U1 1 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD MAR PY 2010 VL 4 IS 1 BP 45 EP 55 DI 10.1007/s11829-010-9086-3 PG 11 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA 562YL UT WOS:000275091800006 ER PT J AU Sie, RS Charles, G Sakhanokho, HF Toueix, Y Dje, Y Sangare, A Branchard, M AF Sie, Raoul Sylvere Charles, Gilbert Sakhanokho, Hamidou F. Toueix, Yannick Dje, Yao Sangare, Abdourahamane Branchard, Michel TI Protocols for callus and somatic embryo initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of explant type, sugar, and plant growth regulators SO AUSTRALIAN JOURNAL OF CROP SCIENCE LA English DT Article DE Malvaceae; tTCL; callogenesis; somatic embryos; plant growth regulator; sugar; explant; Hibiscus sabdariffa L. ID SECONDARY METABOLITES; COTTON GOSSYPIUM; IN-VITRO; CULTURES; EMBRYOGENESIS; REGENERATION; ANTHOCYANINS; ACCUMULATION; EXTRACT; SYSTEM AB A significant work about callus induction and somatic embryogenesis was realized for Hibiscus sabdariffa. Two genotypes (Hibiscus sabdariffa var. sabdariffa and Hibiscus sabdariffa var. altissima), 2 sugars (sucrose and glucose) and three concentrations (1 %, 2%, 3%) of each sugar, 3 explant types (root, hypocotyl, cotyledon) were used for tissue culture. Fourteen combinations of plant growth regulators (PGRs) in MS medium and five combinations of PGR in Driver and Kuniyuki (DKW) medium were tested on hypocotyl and cotyledon for callus and somatic embryo formation. The PGR combinations used with MS medium were naphthaleneacetic acid/kinetin (NAA/KIN), 2,4-dichlorophenoxyacetic acid/kinetin (2,4-D/KIN), and naphthaleneacetic acid/6-benzylaminopurine (NAA/BA) and those used with DKW medium were 2,4-dichlorophenoxyacetic acid/thidiazuron (2,4-D/TDZ). Callus formation was initiated on both genotypes with all concentrations of both sugars and PGRs and in all explant types. The best results for callus induction were achieved with 3% sucrose and the hypocotyl and cotyledon explants. Somatic embryos were obtained with DKW medium supplemented with 4 mg/l 2,4-D + 1 mg/l TDZ and 1 mg/l 2,4-D + 0,5 mg/l TDZ. C1 [Sie, Raoul Sylvere; Dje, Yao] Univ Abobo Adjam, Lab Biol & Ameliorat Prod Vegetales, Abidjan 02, Cote Ivoire. [Sie, Raoul Sylvere; Charles, Gilbert; Toueix, Yannick; Branchard, Michel] Univ Bretagne Occidentale, Lab Biotechnol & Physiol Vegetales, Technopole Brest Iroise, F-29280 Plouzane, France. [Sakhanokho, Hamidou F.] ARS, USDA, Thad Cochran So Hort Lab, Poplarville, MS 39470 USA. [Sangare, Abdourahamane] Ctr Natl Rech Agron, Lab Cent Biotechnol, Abidjan 01, Cote Ivoire. [Charles, Gilbert] Univ Europeenne Bretagne, Bretagne, France. RP Sie, RS (reprint author), Univ Abobo Adjam, Lab Biol & Ameliorat Prod Vegetales, 02 BP 801, Abidjan 02, Cote Ivoire. EM sieraoul@yahoo.fr NR 36 TC 7 Z9 7 U1 0 U2 6 PU SOUTHERN CROSS PUBL PI LISMORE PA 8 91-93 MCKENZIE ST, LISMORE, NSW 2480, AUSTRALIA SN 1835-2693 J9 AUST J CROP SCI JI Aust. J. Crop Sci. PD MAR PY 2010 VL 4 IS 2 BP 98 EP 106 PG 9 WC Agronomy SC Agriculture GA 578CP UT WOS:000276271300006 ER PT J AU Spackman, E Day, JM Pantin-Jackwood, MJ AF Spackman, Erica Day, J. Michael Pantin-Jackwood, Mary J. TI Astrovirus, Reovirus, and Rotavirus Concomitant Infection Causes Decreased Weight Gain in Broad-Breasted White Poults SO AVIAN DISEASES LA English DT Article DE astrovirus; enteric disease; reovirus; rotavirus; turkeys ID TURKEY ORIGIN REOVIRUSES; ENTERIC VIRUSES; PATHOGEN-FREE; TISSUE DISTRIBUTION; MORTALITY SYNDROME; AGE-GROUPS; CHICKENS; PATHOLOGY; COMPLEX; FLOCKS AB Turkey astrovirus type-2 (TAstV-2), turkey rotavirus (TRotV), and turkey reovirus (TReoV) have been implicated as possible causes of enteric diseases and poor production in turkeys; however, numerous studies with each individual virus have failed to reproduce the disease as observed in the field. Therefore, in this study we evaluated the pathogenesis of all possible combinations of one, two, or three viruses in comparison to sham inoculates in 3-day-old turkey poults. Body weights were recorded at 2, 4, 7, 10, and 14 days postinoculation (PI) and were decreased in virus-infected turkeys throughout the experiment as compared to sham inoculates. Although not significantly different from the other virus-exposed groups, the poults exposed to all three viruses had the lowest body weights throughout the experiment. Clinical signs, including huddling, diarrhea, and agitation, were only observed in groups exposed to TAstV-2 and/or TRotV. At 4 days PI, birds from each treatment group were necropsied, and pale intestines with watery contents and undigested feed were observed in the groups that were exposed to TRotV + TReoV or TRotV + TAstV-2 and the group exposed to all three viruses. Minimal microscopic lesions were observed in the intestines of turkeys infected with TAstV-2, TReoV, or a combination of both. In the turkeys infected with TRotV, either alone or in combination with other viruses, mild microscopic lesions were found in all sections of the small intestine and viral antigen was identified by immunohistochemical staining in mature enterocytes. No or very mild lesions were observed in other organs with the exception of the bursa of Fabricius, where mild to severe atrophy was observed in all virus-infected poults examined. Cloacal shedding of TAstV-2 and TRotV was evaluated by reverse-transcription PCR testing of cloacal swabs and minimal differences were observed among the treatment groups. C1 [Spackman, Erica; Day, J. Michael; Pantin-Jackwood, Mary J.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Spackman, E (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Erica.Spackman@ars.usda.gov FU USDA/ARS CRIS [6612-32000-054] FX We gratefully acknowledge Scott Lee, Kimberley Hill, and Diane Smith for technical assistance with this work. The work was supported by USDA/ARS CRIS project #6612-32000-054. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 26 TC 25 Z9 26 U1 1 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 BP 16 EP 21 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 581AR UT WOS:000276492900002 PM 20408393 ER PT J AU Jackwood, MW Suarez, DL Hilt, D Pantin-Jackwood, MJ Spackman, E Woolcock, P Cardona, C AF Jackwood, Mark W. Suarez, David L. Hilt, Deborah Pantin-Jackwood, Mary J. Spackman, Erica Woolcock, Peter Cardona, Carol TI Biologic Characterization of Chicken-Derived H6N2 Low Pathogenic Avian Influenza Viruses in Chickens and Ducks SO AVIAN DISEASES LA English DT Article DE avian influenza virus; H6N2; chickens; ducks; California live bird market; biologic characterization; pathogenicity ID NORTH; REASSORTMENT; CALIFORNIA; AMERICA; H9N2 AB Low pathogenic avian influenza H6N2 viruses were biologically characterized by infecting chickens and ducks in order to compare adaptation of these viruses in these species. We examined the clinical signs, virus shedding, and immune response to infection in 4-wk-old white leghorn chickens and in 2-wk-old Pekin ducks. Five H6N2 viruses isolated between 2000 and 2004 from chickens in California, and one H6N2 virus isolated from chickens in New York in 1998, were given intrachoanally at a dose of 1 x 10(6) 50% embryo infectious dose per bird. Oral-pharyngeal and cloacal swabs were taken at 2, 4, and 7 days postinoculation (PI) and tested by real-time reverse-transcriptase polymerase chain reaction for presence of virus. Serum was collected at 7, 14, and 21 days PI and examined for avian influenza virus antibodies by commercial enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) testing. Virus shedding for all of the viruses was detected in the oral-pharyngeal swabs from chickens at 2 and 4 days PI, but only three of the five viruses were detected at 7 days PI. Only two viruses were detected in the cloacal swabs from the chickens. Virus shedding for four of the five viruses was detected in the oral-pharyngeal cavity of the ducks, and fecal shedding was detected for three of the viruses (including the virus not shed by the oral-pharyngeal route) in ducks at 4 and 7 days PI. All other fecal swabs from the ducks were negative. Fewer ducks shed virus compared to chickens. Both the chickens and the ducks developed antibodies, as evidenced by HI and ELISA titers. The data indicate that the H6N2 viruses can infect both chickens and ducks, but based on the number of birds shedding virus and on histopathology, the viruses appear to be more adapted to chickens. Virus shedding, which could go unnoticed in the absence of clinical signs in commercial chickens, can lead to transmission of the virus among poultry. However, the viruses isolated in 2004 did not appear to replicate or cause more disease than earlier virus isolates. C1 [Jackwood, Mark W.; Hilt, Deborah] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA 30602 USA. [Suarez, David L.; Pantin-Jackwood, Mary J.; Spackman, Erica] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Woolcock, Peter] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Fresno Lab, Fresno, CA 93725 USA. [Cardona, Carol] Univ Calif Davis, Davis, CA 95616 USA. RP Jackwood, MW (reprint author), Univ Georgia, Coll Vet Med, Dept Populat Hlth, 953 Coll Stn Rd, Athens, GA 30602 USA. EM mjackwoo@uga.edu NR 15 TC 13 Z9 14 U1 0 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 BP 120 EP 125 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 581AR UT WOS:000276492900019 PM 20408410 ER PT J AU Senne, DA AF Senne, Dennis A. TI Avian Influenza in North and South America, the Caribbean, and Australia, 2006-2008 SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; highly pathogenic notifiable avian influenza; low pathogenicity avian influenza; low pathogenicity notifiable avian influenza; poultry; surveillance; wild birds ID WATERFOWL; OUTBREAK; VIRUS AB Between 2006 and 2008, only one outbreak of highly pathogenic notifiable avian influenza (AI) was reported from the Americas, the Caribbean, and Australia. on caused by H7N3, occurred in September 2007 in a multiage broiler breeder facility (similar to 49,000 birds) near Regina Beach in southern Saskarchewan, Canada. The disease was confined to a single farm; the farm was depopulated. All other reports of infections in poultry or send birds involved lose pathogenicity AI viruses. A notable event that occurred during the 3-yr period was the spread of low pathogenicity notifiable AI (LPNAI) H5N2 (Mexican lineage) into the Caribbean countries of the Dominican Republic and Haiti in 2007 and 2008, respectively, representing the first detection of AI reported in these countries. Mexico reported that the LPNAI H5N2 virus continued to circulate in the central regions of the country, and a total of 49 isolations were made from 12 states between 2006 and 2008. Also, during this period there was a significant increase in AI surveillance in many countries throughout the Americas, the Caribbean, and Australia, resulting in the detection of AI subtypes H1 through H12 and N1 through N9 in domestic bird species (chickens, turkeys, guinea fowl, upland game birds, and ducks/geese). The United States was the only one of these countries that reported detections of LPNAI (H5 or H7) infections in commercial poultry: one in chickens (H7N3, 2007), two in turkeys (H5N1 and H5N2, 2007), and one in pheasants (H5N8, 2008). Detections of AI viruses in wild birds between 2006 and 2008 were reported from North America (Canada and the United States), South America (Bolivia, Argentina; Chile, and Brazil), and Australia. C1 USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA. RP Senne, DA (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA. EM dennis.a.senne@aphis.usda.gov NR 6 TC 20 Z9 21 U1 1 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 179 EP 186 DI 10.1637/8921-050809-Review.1 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300004 PM 20521630 ER PT J AU Pfeiffer, J Suarez, DL Sarmento, L To, TL Nguyen, T Pantin-Jackwood, MJ AF Pfeiffer, Jennifer Suarez, David L. Sarmento, Luciana To, Thanh Long Nguyen, Tung Pantin-Jackwood, Mary J. TI Efficacy of Commercial Vaccines in Protecting Chickens and Ducks Against H5N1 Highly Pathogenic Avian Influenza Viruses from Vietnam SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; vaccine; chickens; ducks; H5N1; highly pathogenic ID NEWCASTLE-DISEASE VIRUS; OIL-EMULSION VACCINES; HONG-KONG; A VIRUSES; INACTIVATED VACCINES; HEMAGGLUTININ GENE; MALLARD DUCKS; PEKIN DUCKS; VACCINATION; EVOLUTION AB Highly pathogenic (HP) H5N1 avian influenza (AI) viruses continue to circulate in Asia and have spread to other regions of the world. Though attempts at eradication of the viruses during various outbreaks have been successful for short periods of rime, new strains of H5N1 viruses continue to emerge and have become endemic in parts of Asia and Africa. Vaccination has been employed in Vietnam as part of AI control programs. Domestic ducks, which make up a large part of poultry in Vietnam, have been recognized as one of the primary factors in the spread of AI in this country. As a result, ducks have been included in the vaccination programs. Despite the effort to control AI in Vietnam, eradication of the disease has not been possible, due in part to the emergence and spread of new viruses. Here, we tested the abilities of avian influenza oil emulsion vaccines of different genetic origins to protect against disease and viral shedding in both 2-wk-old white leghorn chickens and 1-wk-old Pekin ducks. Seventy-five to 100% of vaccinated chickens were protected from mortality, but viral shedding occurred for at least 4 days post challenge. All but one vaccinated duck were protected from mortality; however, all groups shed virus up through at least 5 days postchallenge, depending on the vaccine and challenge virus used. Differences in levels of hemagglutination inhibition (HI) antibody titers induced by the vaccines were observed in both chickens and ducks. Although the vaccines tested were effective in protecting against disease and mortality, updated and more efficacious vaccines are likely needed to maintain optimal protection. C1 [Pfeiffer, Jennifer; Suarez, David L.; Sarmento, Luciana; Pantin-Jackwood, Mary J.] USDA, SE Poultry Res Lab, ARS, Athens, GA 30605 USA. [To, Thanh Long; Nguyen, Tung] Natl Ctr Vet Diag, Dept Anim Hlth, Hanoi, Vietnam. RP Pantin-Jackwood, MJ (reprint author), USDA, SE Poultry Res Lab, ARS, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ars.usda.gov NR 62 TC 29 Z9 29 U1 1 U2 10 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 262 EP 271 DI 10.1637/8715-031909-Reg.1 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300017 PM 20521643 ER PT J AU Avellaneda, G Sylte, MJ Lee, CW Suarez, DL AF Avellaneda, Gloria Sylte, Matt J. Lee, Chang-Won Suarez, David L. TI A Heterologous Neuraminidase Subtype Strategy for the Differentiation of Infected and Vaccinated Animals (DIVA) for Avian Influenza Virus Using an Alternative Neuraminidase Inhibition Test SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE influenza A virus; DIVA strategy; neuraminidase inhibition test; diagnostic; chickens ID ORIGINAL ANTIGENIC SIN; NONSTRUCTURAL PROTEIN; HONG-KONG; CHICKENS; ANTIBODIES; EFFICACY; POULTRY; H5; GENERATION; PROTECTION AB The option of vaccinating poultry against avian influenza (AI) as a control tool is gaining greater acceptance by governments and the poultry industry worldwide. One disadvantage about vaccination with killed whole-virus vaccines is the resulting inability to use common serologic diagnostic tests for surveillance to identify infected flocks. There has been considerable effort to develop a reliable test for the differentiation of infected from vaccinated animals (DIVA). The heterologous neuraminidase (NA) subtype DIVA approach has been used with some success in the field accompanied by an ad hoc serologic test. The traditional NA inhibition (NI) test can be used for all nine NA subtypes, but it is time consuming, and it is not designed to screen large numbers of samples. In this study, a quantitative NI test using MUN (2'[4-methylumbelliferyl]-alpha-D-Nacetylneuraminic acid sodium salt hydrate) as an NA substrate was investigated as an alternative to the traditional fetuin-based NI test in a heterologous neuraminidase DIVA strategy. Serum NI activity was determined in chickens administered different vaccines containing different H5 and NA subtypes and challenged with a highly pathogenic avian influenza (HPAI) H5N2 virus. Prior to challenge, the NI DIVA test clearly discriminated between chickens receiving vaccines containing different antigens (e.g., N8 or N9) from control birds that had no NA antibody. Some birds began to seroconvert 1 wk postchallenge, and 100% of the vaccinated birds had significant levels of N2 NI activity. This activity did not interfere with the presence of vaccine-induced NI activity against N8 or N9 subtypes. The level of N2-specific NI activity continued to increase to the last sampling date, 4 wk postchallenge, indicating the potential use for the heterologous NA-based DIVA strategy in the field. C1 [Avellaneda, Gloria; Suarez, David L.] USDA, SE Poultry Res Lab, ARS, Athens, GA 30605 USA. [Sylte, Matt J.] Univ Georgia, Sch Vet Med, Dept Infect Dis, Athens, GA 30605 USA. [Lee, Chang-Won] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Wooster, OH 44691 USA. RP Suarez, DL (reprint author), USDA, SE Poultry Res Lab, ARS, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.suarez@ars.usda.gov NR 38 TC 6 Z9 7 U1 0 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 272 EP 277 DI 10.1637/8677-030409-Reg.1 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300018 PM 20521644 ER PT J AU Avellaneda, G Mundt, E Lee, CW Jadhao, S Suarez, DL AF Avellaneda, Gloria Mundt, Egbert Lee, Chang-Won Jadhao, Samadhan Suarez, David L. TI Differentiation of Infected and Vaccinated Animals (DIVA) Using the NS1 Protein of Avian Influenza Virus SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE influenza; DIVA; NS1; ELISA; poultry ID A VIRUS; NONSTRUCTURAL PROTEIN; MOUTH-DISEASE; CHICKENS; ANTIBODIES; STRATEGY; RECOMBINANT; H5; DISTINCTION; GENERATION AB Vaccination against avian influenza (AI) virus, a powerful tool for control of the disease, may result in issues related to surveillance programs and international trade of poultry and poultry products. The use of AI vaccination in poultry would have greater worldwide acceptance if a reliable test were available that clearly discriminated between naturally infected and vaccinated-only animals (DIVA). Because the nonstructural protein (NS1) is expressed in influenza virus infected cells, and it is not packaged in the virion, it is an attractive candidate for a DIVA differential diagnostic test. The aim of this work was to determine the onset of the antibody response to the NS1 protein in chickens infected with low pathogenic avian influenza (LPAI) virus, and to evaluate the diagnostic potential of a baculovirus-expressed purified NS1 protein in an indirect ELISA-based DIVA strategy. An antibody response against NS1 was first detected 3 wk after infection, but the antibody levels were decreasing rapidly by 5 wk after infection. However, most chickens did not have detectable antibodies in spite of high hemagglutination inhibition (HI) antibody titers in one group. In birds vaccinated with inactivated oil-emulsion vaccines, antibodies against NS1 were not detected before virulent challenge, and only a small percentage of birds scroconverted after homologous LPAI virus challenge. Vaccinated birds challenged with highly pathogenic AI showed a higher NS1 antibody response, but at most only 40% of birds seroconverted against NS1 protein by 3 wk after challenge. Because of the variability of seroconversion and the duration of the antibody response in chickens, the NS1 protein DIVA strategy did not perform as well as expected, and if this strategy were to be used, it would require sampling a higher number of birds to compensate for the lower seroconversion rate. C1 [Avellaneda, Gloria; Jadhao, Samadhan; Suarez, David L.] USDA ARS, SE Poultry Res Lab, ARS, Athens, GA 30605 USA. [Mundt, Egbert] Univ Georgia, Sch Vet Med, Dept Infect Dis, Athens, GA 30605 USA. [Lee, Chang-Won] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Wooster, OH 44691 USA. RP Suarez, DL (reprint author), USDA ARS, SE Poultry Res Lab, ARS, 934 Coll Stn Rd, Athens, GA 30605 USA. EM David.Suarez@ars.usda.gov NR 36 TC 13 Z9 15 U1 0 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 278 EP 286 DI 10.1637/8644-020409-Reg.1 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300019 PM 20521645 ER PT J AU Jadhao, SJ Suarez, DL AF Jadhao, Samadhan J. Suarez, David L. TI New Approach to Delist Highly Pathogenic Avian Influenza Viruses from BSL3+Select Agents to BSL2 Non-Select Status for Diagnostics and Vaccines SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza virus; reverse genetics; NS1 gene; avian influenza vaccine; select agents ID REVERSE GENETICS; HEMAGGLUTININ GENE; H5N1 VIRUSES; A VIRUSES; HONG-KONG; AFRICA; EVOLUTION; CHICKENS; NIGERIA; CHINA AB Highly pathogenic avian influenza viruses (AIVs) arc Select Agents in the United States and are required to be handled in bio-containment level-3 enhanced (BSE3+) facilities. Using a reverse genetics system, we attenuated a highly pathogenic virus, with the goal of making it low pathogenic and having it delisted as a Select Agent so that it could be handled in a bio-containment level-2 facility for diagnostic or vaccine production applications. We utilized two approaches to attenuate the target AIV by mutating the highly pathogenic hemagglutinin (HA) cleavage site to be low pathogenic and by replacing the full-length NS gene segment with a naturally truncated 124 amino acid NSI coding gene from A/turkey/Oregon/73 (H7N3) virus (tkOR71 trNS1). To delist an AIV so that it can be handled in a BSL2 facility, the amino acid sequence of the HA cleavage site of the rescued virus must be confirmed to be compatible with a low-pathogenic AIV; it should not plaque in cell culture without supplementation of exogenous trypsin; and intravenous pathotyping in 4-6-wk-old specific-pathogen-free chickens must confirm that the virus is low pathogenic. The candidate A/duck/Vietnana/Baclieu/09/07 (rH5N1/PR8/trNS1) virus with five PR8 internal genes, tkOR71 trNS1 gene, and A/chicken/Indonesia/7/03 NI neuraminidase gene was constructed. The virus was shown to not plaque in cell culture without addition of trypsin. The virus was low pathogenic in the standard intravenous pathotyping rest (IVPI = 0) and also caused no disease in a separate intranasal inoculation test in 4-wk-old specific-pathogen-free chickens, thus demonstrating that the virus is storable for deselection. C1 [Jadhao, Samadhan J.; Suarez, David L.] USDA, ARS, SE Poultry Res Lab, Athens, GA 30605 USA. RP Suarez, DL (reprint author), USDA, ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.suarez@ars.usda.gov NR 29 TC 2 Z9 2 U1 1 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 302 EP 306 DI 10.1637/8926-051509-ResNote.1 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300024 PM 20521650 ER PT J AU Arafa, A Suarez, DL Hassan, MK Aly, MM AF Arafa, A. Suarez, D. L. Hassan, M. K. Aly, M. M. TI Phylogenetic Analysis of Hemagglutinin and Neuraminidase Genes of Highly Pathogenic Avian Influenza H5N1 Egyptian Strains Isolated from 2006 to 2008 Indicates Heterogeneity with Multiple Distinct Sublineages SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE highly pathogenic avian influenza; subtype H5N1 virus; phylogenetic analyses; hemagglutinin; neuraminidase ID A VIRUSES; OUTBREAKS; POULTRY; ASIA AB The Eurasian-lineage H5N1 highly pathogenic avian influenza (HPAI) virus caused widespread outbreaks in Egypt in 2006 and eventually become enzootic in poultry. Although outbreaks have a seasonal pattern, with most occurring during the cooler winter months, it remains unclear whether this seasonality reflects virus maintenance within Egypt or yearly introductions of the virus into the country. To evaluate the epidemiology of H5N1 HPAI in Egypt, sequence analysis of the hemagglutinin (HA) and neuraminidase (NA) genes of selected Egyptian isolates from early 2006 to 2008 was conducted. The data from this study identifies distinct genetic markers in both HA and NA genes and suggests grouping Egyptian isolates into two major HA isolate sublineages from 2006 to 2008 and into three smaller, emergent subgroups. The NA phylogenetic and sequence analysis showed a similar pattern, except that two of the emergent groups from the HA phylogenetic tree clustered together, evidence of likely reassortment. The different subgroups did not appear to segregate by relation to the date of isolation, to the species of origin, nor to the geographic location of the viruses. The conclusion is that H5N1 is continuing to mutate with multiple heterogenic strains persisting in Egypt. C1 [Arafa, A.; Hassan, M. K.; Aly, M. M.] Natl Lab Vet Qual Control Poultry Prod, Giza 12618, Egypt. [Suarez, D. L.] Agr Res Serv, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Arafa, A (reprint author), Natl Lab Vet Qual Control Poultry Prod, POB 246, Giza 12618, Egypt. EM araby85@hotmail.com NR 13 TC 41 Z9 41 U1 1 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 345 EP 349 DI 10.1637/8927-051509-ResNote.1 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300031 PM 20521657 ER PT J AU Pedersen, K Swafford, SR DeLiberto, TJ AF Pedersen, Kerri Swafford, Seth R. DeLiberto, Thomas J. TI Low Pathogenicity Avian Influenza Subtypes Isolated from Wild Birds in the United States, 2006-2008 SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; mallards; subtypes; surveillance; virus isolation; wild birds ID A VIRUSES; RESIDENT DUCKS; NORTH-AMERICA; AQUATIC BIRDS; WATERFOWL; PARAMYXOVIRUSES; SURVEILLANCE; EVOLUTION; OUTBREAK; AREAS AB Due to concerns that high pathogenicity, avian influenza would enter into the United Stares, an interagency strategic plan was developed to conduct surveillance in wild birds in order to address one of the possible pathways of entry. The USDA and state wildlife agencies participated in this effort by collecting samples front 145,055 wild birds from April 2006 through March 2008 in all 50 states. The majority (59%) of all wild bird samples was collected from dabbling ducks, and 91 Vs of detections using real-time reverse transcriptase polymerase chain reaction (rRT-PCR) were in dabbling ducks. Apparent prevalence of 115 by rRT-PCR in all birds sampled was 0.38%. Most (48%) H5 detections were found in mallards (Anus platyrhynchos). Thirty-three virus subtypes were identified; H5N2 was the most prevalent subtype and accounted for 40% of all virus isolations. We present the virus subtypes obtained from the national surveillance effort and compare them with research results published from various countries. C1 [Pedersen, Kerri; Swafford, Seth R.; DeLiberto, Thomas J.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Ft Collins, CO 80521 USA. RP Pedersen, K (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Kerri.Pedersen@aphis.usda.gov NR 39 TC 11 Z9 12 U1 0 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 405 EP 410 DI 10.1637/8693-031309-Reg.1 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300044 PM 20521670 ER PT J AU Lvov, DK Shchelkanov, MY Prilipov, AG Vlasov, NA Fedyakina, IT Deryabin, PG Alkhovsky, SV Grebennikova, IV Zaberezhny, AD Suarez, DL AF Lvov, D. K. Shchelkanov, M. Yu Prilipov, A. G. Vlasov, N. A. Fedyakina, I. T. Deryabin, P. G. Alkhovsky, S. V. Grebennikova, I. V. Zaberezhny, A. D. Suarez, D. L. TI Evolution of Highly Pathogenic Avian Influenza H5N1 Virus in Natural Ecosystems of Northern Eurasia (2005-08) SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE virus; HPAI; H5N1; natural ecosystems; northern Eurasia; evolution ID A-VIRUS; BIRDS; AFRICA; A/H5N1; TERRITORY; VIRULENCE; OUTBREAK; EPIZOOTY; SIBERIA; DUCKS AB Fifty-four strains of H5N1 highly pathogenic avian influenza (HPAI) virus were isolated from wild birds in the ecosystems of northern Eurasia and from poultry in the south of western Siberia (July 2005), at the mouth of Volga River (November 2005), at Uvs-Nur Lake on the boundary of the Great Lakes Depression in western Mongolia and the Tyva Republic of Russia (June 2006), in the vicinity of Moscow (February 2007), in the southeastern parr of the Russian Plain (September 2007 and December 2007), and in the far east (April 2008) of the Russian Federation and were phenotypically characterized and deposited into the Russian state collection or viruses. Complete genome nucleotide sequences for 24 strains were obtained and deposited into GenBank. In all cases when strains were isolated from both wild birds and poultry in the same outbreak these strains were genetically closely related to each other. Until 2008 all HPAI H5N1 strains isolated in northern Eurasia clustered generically with the viruses from Kukunor Lake (Qinghai Province, China), known as genotype 2.2 or the "Qinghai-Siberian" genotype. The viruses from the Qinghai-Siberian genotype have continued to evolve from those initially introduced into western Siberia in 2005 into two genetic groups: "Iran-North Caucasian" and "Tyva-Siberian." In vitro replication potential (50% tissue-culture infectious dose in porcine embryo kidney) of Qinghai-Siberian strains decreased over rime, which could reflect decreasing virulence. Comparison of genome sequences with biological characteristics of the respective strains permitted us to identify point mutations in PB2, PB1, PA, HA, NP, NA, M2, NS1, and NS2 that possibly influenced the level of replication potential. The HPAI H5N1 virus, which penetrated into the south of the Russian Far East in spring 2008, belonged to genotype 2.3.2. C1 [Lvov, D. K.; Shchelkanov, M. Yu; Prilipov, A. G.; Vlasov, N. A.; Fedyakina, I. T.; Deryabin, P. G.; Alkhovsky, S. V.; Grebennikova, I. V.; Zaberezhny, A. D.] Russian Acad Med Sci, DI Ivanovskii Virol Inst, Moscow 123098, Russia. [Suarez, D. L.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. RP Lvov, DK (reprint author), Russian Acad Med Sci, DI Ivanovskii Virol Inst, 16 Gamalei St, Moscow 123098, Russia. EM dk_lvov@mail.ru RI Alkhovsky, Sergey/A-9542-2014; Shchelkanov, Mikhail/L-6164-2016; OI Shchelkanov, Mikhail/0000-0001-8610-7623; Deryabin, Petr/0000-0002-8522-9554; Grebennikova, Т. V./0000-0002-6141-9361 NR 40 TC 9 Z9 24 U1 1 U2 11 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 483 EP 495 DI 10.1637/8893-042509-Review.1 PG 13 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300057 PM 20521683 ER PT J AU Ramirez-Nieto, G Shivaprasad, HL Kim, CH Lillehoj, HS Song, HC Osorio, IG Perez, DR AF Ramirez-Nieto, Gloria Shivaprasad, H. L. Kim, Chul-Hong Lillehoj, Hyun Soon Song, Haichen Osorio, Ivan Gomez Perez, Daniel R. TI Adaptation of a Mallard H5N2 Low Pathogenicity Influenza Virus in Chickens with Prior History of Infection with Infectious Bursal Disease Virus SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; adaptation; infectious bursal disease virus; pathogenesis ID AVIAN INFLUENZA; A VIRUSES; HEMAGGLUTININ; TRANSMISSION; PATHOGENESIS; EMERGENCE; IMMUNOSUPPRESSION; PATHOBIOLOGY; PENNSYLVANIA; RESPONSES AB The influenza A/Mallard/Pennsylvania/10218/1984 (H5N2) virus is unable to replicate in 3-wk-old immunocompetent specific-pathogen-free chickens when a dose of 5 x 10(6) 50% egg infectious dose/ml is used. In contrast, this mallard virus shows limited replication in 3-wk-old chickens that had been previously infected at 2 days of age with, and recovered from, the immunosuppressive agent infectious bursal disease virus (IBDV; herein referred to as IBDV chickens). This limited replication in IBDV chickens allowed for the serial passage of the mallard influenza virus in chickens. After 22 passages (P22) in IBDV chickens, the resulting chicken-adapted influenza virus replicated in both immunocompetent and IBDV chickens more efficiently than the mallard influenza virus. Analysis of the outcomes of infection and the lesions caused by the two viruses at the microscopic level in a time-point study showed that the P22 virus is more virulent than the parental mallard virus in both immunocompetent and IBDV chickens. Our studies provide evidence that a previous history of IBDV infection in chickens may render them more susceptible to avian influenza virus (AIV) infections, allowing for the potential introduction of AIVs in an otherwise resistant population. C1 [Ramirez-Nieto, Gloria; Song, Haichen; Osorio, Ivan Gomez; Perez, Daniel R.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. [Ramirez-Nieto, Gloria; Song, Haichen; Osorio, Ivan Gomez; Perez, Daniel R.] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA. [Shivaprasad, H. L.] Univ Calif Davis, Fresno Branch, Calif Anim Hlth & Food Safety Lab Syst, Fresno, CA 93725 USA. [Kim, Chul-Hong; Lillehoj, Hyun Soon] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Ramirez-Nieto, Gloria] Univ Nacl Colombia, Fac Med Vet & Zootecnia, Bogota, Colombia. RP Perez, DR (reprint author), Univ Maryland, Dept Vet Med, 8075 Greenmead Dr, College Pk, MD 20742 USA. EM dperez1@umd.edu OI Perez, Daniel/0000-0002-6569-5689 FU AHRQ HHS [HHSN266200700010C]; NIAID NIH HHS [R01 AI052155, R01AI052155] NR 29 TC 9 Z9 11 U1 1 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 513 EP 521 DI 10.1637/8902-042809-Reg.1 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300061 PM 20521687 ER PT J AU Pillai, SPS Pantin-Jackwood, M Yassine, HM Saif, YM Lee, CW AF Pillai, S. P. S. Pantin-Jackwood, M. Yassine, H. M. Saif, Y. M. Lee, C. W. TI The High Susceptibility of Turkeys to Influenza Viruses of Different Origins Implies Their Importance as Potential Intermediate Hosts SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE turkeys; influenza; interspecies transmission ID LIVE BIRD MARKETS; AVIAN INFLUENZA; A VIRUSES; UNITED-STATES; SWINE INFLUENZA; INTERSPECIES TRANSMISSION; BREEDER HENS; RECEPTOR SPECIFICITY; SENTINEL DUCKS; TARGET-CELLS AB Several previous reports and our studies show that waterfowl-origin influenza viruses can be more easily transmitted to domestic turkeys than chickens. Similarly, studies indicate turkeys to be better hosts for low pathogenic avian influenza viruses isolated from commercial poultry operations and live bird markers in comparison to chickens. Low 50% infectious-dose titers of wild bird as well as poultry-adapted viruses for turkeys further suggest that turkeys can be easily infected following a low-dose exposure. Also, interspecies transmission of swine influenza viruses to turkeys occurs frequently. These findings suggest the role of turkeys as suitable intermediate hosts that can be easily infected with influenza viruses of different origins and that turkeys can act as source of infection for other land-based poultry or even mammals. C1 [Pillai, S. P. S.; Yassine, H. M.; Saif, Y. M.; Lee, C. W.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Columbus, OH 43210 USA. [Pillai, S. P. S.; Yassine, H. M.; Saif, Y. M.; Lee, C. W.] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Pantin-Jackwood, M.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. RP Lee, CW (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, 1680 Madison Ave, Wooster, OH 44691 USA. EM lee.2854@osu.edu NR 66 TC 22 Z9 23 U1 0 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 522 EP 526 DI 10.1637/8770-033109-Review.1 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300062 PM 20521688 ER PT J AU Sarmento, L Wasilenko, J Pantin-Jackwood, M AF Sarmento, Luciana Wasilenko, Jamie Pantin-Jackwood, Mary TI The Effects of NS Gene Exchange on the Pathogenicity of H5N1 HPAI Viruses in Ducks SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE H5N1; avian influenza; NS1; ducks; gene expression ID AVIAN INFLUENZA-VIRUS; ANTIVIRAL CYTOKINE RESPONSES; A VIRUSES; HONG-KONG; PATHOBIOLOGY; VIRULENCE AB Until 2002, H5N1 highly pathogenic avian influenza (HPAI) viruses caused only mild respiratory infections in ducks. Since then, new viruses have emerged that cause systemic disease and high mortality in ducks and other waterfowl. Studies on HPAI virus pathogenicity in ducks have been limited, and there is no clear explanation of why the pathogenicity of some H5N1 HPAI viruses has increased. The nonstructural protein 1 (NS1 protein) is known to suppress immune responses in influenza virus infected hosts affecting virus pathogenesis. In order to determine if the NS1 protein contributes to increased virulence in ducks, single-gene reassortant viruses were generated. Exchanging the NS genes from A/Ck/HK/220/97 (a virus that produces mild disease in ducks) and A/Dk/VN/201/05 (a very virulent virus for ducks) in the rEgret/02 background (a recombinant virus derived from A/ Egret/HK/757.2/02, a highly pathogenic virus in ducks) resulted in decreased mean death times compared to infection with the rEgret/02 virus in ducks, but the change was not statistically significant. Infection with the reassortant viruses affected the expression of immune-related genes in spleens and lungs when compared to controls, but when compared among them, the expression of the duck genes was similar. Furthermore, virus titers in spleen, lung, and brain as well as antigen distribution in various tissues were similar in ducks infected with the reassortant viruses. All together these data show that, under these experimental conditions, exchanging the NS gene had minimal effect on the virus pathogenicity, and it suggests that other viral genes, or combination of genes, are most likely contributing to the increased virulence of H5N1 HPAI viruses in ducks. C1 [Sarmento, Luciana; Wasilenko, Jamie; Pantin-Jackwood, Mary] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Pantin-Jackwood, M (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ars.usda.gov NR 25 TC 9 Z9 10 U1 0 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 532 EP 537 DI 10.1637/8917-050409-Reg.1 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300064 PM 20521690 ER PT J AU Hunt, HD Jadhao, S Swayne, DE AF Hunt, Henry D. Jadhao, Samadhan Swayne, David E. TI Major Histocompatibility Complex and Background Genes in Chickens Influence Susceptibility to High Pathogenicity Avian Influenza Virus SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza virus; major histocompatibility complex; B-complex; B congenics ID INFECTIOUS-BRONCHITIS VIRUS; BURSAL DISEASE VIRUS; MX GENE; IMMUNE-RESPONSE; MAREKS-DISEASE; RESISTANCE; LINES; HAPLOTYPES; TRAITS AB The chicken's major histocompatibility complex (MHC) haplotype has profound influence on the resistance or susceptibility to certain pathogens. For example, the B21 MHC haplotype confers resistance to Marek's disease (MD). However, non-MHC genes arc also important in disease resistance. For example, lines 6 and 7 both express the 02 MHC haplotype, but differ in non-MHC genes. Line 6, but not line 7, is highly resistant to tumors induced by the Marek's disease herpesviruses and avian leukosis retroviruses. Recently, survival in the field by Thai indigenous chickens to H5N1 high-pathogenicity avian influenza (HPAI) outbreaks was attributed to the 1321 MHC haplotype, whereas the B13 MHC haplotype was associated with high mortality in the field. To determine the influence of the MHC haplotype on HPAI resistance, a series of MHC congenic white leghorn chicken lines (02, 012, B13, B19, and B21) and lines with different background genes but with the same B2 MHC haplotype (Line 6(3) and 7(1)) were intranasally challenged with low dose (10 mean chicken lethal doses) of reverse-genetics derived rg-A/chicken/ Indonesia/7/2003 (H5N1) HPAI virus. None of the lines were completely resistant to lethal effects of the challenge, as evidenced by mortality rates ranging from 40% to 100%. The B21 line had mortality of 40% and 70%, and the B13 line had mortality of 60% and 100% in two separate trials. In addition, the mean death times varied greatly between groups, ranging from 3.7 to 6.9 days, suggesting differences in pathogenesis. The data show that the MHC has some influence on resistance to AI, but less than previously proposed, and non-MHC background genes may have a bigger influence on resistance than the MHC. C1 [Jadhao, Samadhan; Swayne, David E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Hunt, Henry D.] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Swayne, DE (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.swayne@ars.usda.gov NR 21 TC 5 Z9 5 U1 0 U2 7 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 572 EP 575 DI 10.1637/8888-042409-ResNote.1 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300070 PM 20521696 ER PT J AU Lira, J Moresco, KA Stallknecht, DE Swayne, DE Fisher, DS AF Lira, Jennifer Moresco, Kira A. Stallknecht, David E. Swayne, David E. Fisher, Dwight S. TI Single and Combination Diagnostic Test Efficiency and Cost Analysis for Detection and Isolation of Avian Influenza Virus from Wild Bird Cloacal Swabs SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza virus; diagnostic tests; real-time reverse transcriptase-PCR; surveillance; wild birds; yolk sac inoculation ID A VIRUSES; PCR; INFECTIONS; PREVALENCE; ALASKA; GENES; DUCKS AB Effective laboratory methods for identifying avian influenza virus (ICY) in wild bird populations are crucial to understanding the ecology of this pathogen. The standard method has been AIV isolation in chorioallantoic sac (CAS) of specific-pathogen-free embryonating chicken eggs (ECE), but in one study, combined use of yolk-sac (YS) and chorioallantoic membrane inoculation routes increased the number of virus isolations. In addition, cell culture for AIV isolation has been used. Most recently, real-time reverse rranscriptase (RRT)-PCR has been used to detect AIV genome in surveillance samples. The purpose of this study was to develop a diagnostic decision tree that would increase AIV isolations from wild bird surveillance samples when using combinations of detection and isolation methods under our laboratory conditions. Attempts to identify AIV for 50 wild bird surveillance samples were accomplished via isolation in ECE using CAS and YS routes of inoculation, and in Madin-Darby canine kidney (MDCK) cells, and by AIV matrix gene detection using RRT-PCR. AIV was isolated from 36% of samples by CAS inoculation and 46% samples by YS inoculation using ECE, isolated from 20% of samples in MDCK cells, and detected in 54% of the samples by RRT-PCR. The AIV was isolated in ECE in 13 samples by both inoculation routes, five additional samples by allantoic, and 10 additional samples by yolk-sac inoculation, increasing the positive isolation of AIV in ECE to 56%. Allantoic inoculation and RRT-PCR detected AIV in 14 samples, with four additional samples by allantoic route alone and 13 additional samples by RRT-PCR. Our data indicate that addition of YS inoculation of ECE will increase isolation of AIV from wild bird surveillance samples. If we exclude the confirmation RT-PCR test, cost analysis for our laboratory indicates that RRT-PCR is an economical choice for screening samples before doing virus isolation in FCE if the AIV frequency is low in the samples. In contrast, isolation in ECE via CAS and YS inoculation routes without prescreening by RRT-PCR was most efficient and cost-effective if the samples had an expected high frequency of AIV. C1 [Lira, Jennifer; Moresco, Kira A.; Swayne, David E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Lira, Jennifer] Univ Georgia, Franklin Coll Arts & Sci, Div Biol Sci, Athens, GA 30602 USA. [Stallknecht, David E.] Univ Georgia, Coll Vet Med, SE Wildlife Cooperat Study, Athens, GA 30602 USA. [Fisher, Dwight S.] USDA ARS, J Phil Campbell Sr Nat Resource Conservat Ctr, Watkinsville, GA 30677 USA. RP Lira, J (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. NR 13 TC 7 Z9 7 U1 3 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 606 EP 612 DI 10.1637/8838-040309-Reg.1 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300076 PM 20521702 ER PT J AU Liu, Y Mundt, E Mundt, A Sylte, M Suarez, DL Swayne, DE Garcia, M AF Liu, Y. Mundt, E. Mundt, A. Sylte, M. Suarez, D. L. Swayne, D. E. Garcia, M. TI Development and Evaluation of an Avian Influenza, Neuraminidase Subtype 1, Indirect Enzyme-Linked Immunosorbent Assay for Poultry Using the Differentiation of Infected from Vaccinated Animals Control Strategy SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; heterologous neuraminidase; ELISA; DIVA ID NONSTRUCTURAL PROTEIN; ANTIBODIES; VIRUS; CHICKENS; INTRODUCTIONS; NS1 AB An indirect enzyme-linked immunosorbent assay (ELISA) was developed using baculovirus, purified, recombinant N1 protein from A/chicken/Indonesia/PA7/2003 (H5N1) virus. The N1-ELISA showed high selectivity for detection of NI antibodies, with no cross-reactivity with other neuraminidase subtypes, and broad reactivity with sera to NI subtype isolates from North American and Eurasian lineages. Sensitivity of the N1-ELISA to detect N1 antibodies in turkey seta, collected 3 wk after H1N1 vaccination, was comparable to detection of avian influenza antibodies by the commercial, indirect ELISAs ProFLOK (R) AIV Plus ELISA Kit (Synbiotics, Kansas City, MO) and Avian Influenza Virus Antibody Test Kit (IDEXX, Westbrook, ME). However, 6 wk after vaccination, the Synbiotics ELISA kit performed better than the N1-ELISA and the IDEXX ELISA kit. An evaluation was made of the ability of the NI-ELISA to discriminate vaccinated chickens front subsequently challenged chickens. Two experiments were conducted, chickens were vaccinated with inactivated H5N2 and H5N9 viruses and challenged with highly pathogenic H5N1 virus, and chickens were vaccinated with recombinant poxvirus vaccine encoding H7 and challenged with highly pathogenic H7N1 virus. Serum samples were collected at 14 days postchallenge and tested by hemagglutination inhibition (HI), quantitative neuraminidase inhibition (NI), and N1-ELISA. At 2 days postchallenge, oropharyngeal swabs were collected for virus isolation (VI) to confirm infection. The N1-ELISA was in Our agreement with VI and HI results. Although the N1-ELISA showed a lower sensitivity than the NI assay, it was demonstrated that detection of N1 antibodies by ELISA was an effective and rapid assay to identify exposure to the challenge virus in vaccinated chickens. Therefore, N1-ELISA can facilitate a vaccination strategy with differentiation of infected from vaccinated animals using a neuraminidase heterologous approach. C1 [Liu, Y.; Mundt, E.; Mundt, A.; Garcia, M.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Poultry Diagnost & Res Ctr, Athens, GA 30602 USA. [Sylte, M.] Univ Georgia, Coll Vet Med, Dept Infect Dis, Athens, GA 30602 USA. [Sylte, M.; Suarez, D. L.; Swayne, D. E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Garcia, M (reprint author), Univ Georgia, Coll Vet Med, Dept Populat Hlth, Poultry Diagnost & Res Ctr, 953 Coll Stn Rd, Athens, GA 30602 USA. EM mcgarcia@uga.edu NR 25 TC 5 Z9 5 U1 0 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 613 EP 621 DI 10.1637/8844-040409-Reg.1 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300077 PM 20521703 ER PT J AU Moresco, KA Stallknecht, DE Swayne, DE AF Moresco, Kira A. Stallknecht, David E. Swayne, David E. TI Evaluation and Attempted Optimization of Avian Embryos and Cell Culture Methods for Efficient Isolation and Propagation of Low Pathogenicity Avian Influenza Viruses SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza virus; cell culture; avian embryo; propagation; detection AB Surveillance of wild bird populations for avian influenza viruses (AIV) contributes to our understanding of AIV evolution and ecology. Both real-time reverse transcriptase polymerase chain reaction (RRT-PCR) and virus isolation in embryonating chicken eggs (ECE) are standard methods for detecting AIV in swab samples from wild birds, but AIV detection rates are higher with RRT-PCR than isolation in ECE. In this study we tested duck embryos, turkey embryos, and multiple cell lines for AIV growth as compared to ECE for improved isolation and propagation of AIV for isolates representing all 16 hemagglutinin subtypes. There were no differences in low pathogenicity AIV (LPAIV) propagation titers in duck or turkey embryos compared to ECE. The replication efficiency of LPAIV was lower in each of the cell lines tested compared to ECE. LPAIV titers were 1-3 log mean tissue-culture infective doses (TCID(50)) lower in Madin-Darby canine kidney (MDCK), primary chicken embryo kidney (CEK), and primary chicken embryo fibroblast (CU) cell cultures. and 3-5 log TCID(50) lower in chicken bone marrow macrophage (HD11), chicken fibroblast (DF-1), and mink lung epithelial (Mv1Lu) cells than the corresponding mean embryo infective doses (EID(50)) in ECE. The quail fibroblast (QT-35) and baby hamster kidney (BHK-21) cell lines produced titers 5-7 log TCID(50) less than EID(50) in ECE. Overall, ECEs were the most efficient system for growth of LPAIV. However, the savings in time and resources incurred with the use of due MDCK, CEK, and CEF cultures would allow a higher volume of samples to be processed with the same fiscal and financial resources, thus being potentially advantageous despite the lower replication efficiency and lower isolation rates. C1 [Moresco, Kira A.; Swayne, David E.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Stallknecht, David E.] Univ Georgia, Coll Vet Med, SE Cooperat Wildlife Dis Study, Athens, GA 30606 USA. RP Moresco, KA (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM kira.moresco@ars.usda.gov NR 9 TC 24 Z9 25 U1 0 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 622 EP 626 DI 10.1637/8837-040309-Reg.1 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300078 PM 20521704 ER PT J AU Van Borm, S Suarez, DL Boschmans, M Ozhelvaci, O Marche, S van den Berg, TP AF Van Borm, Steven Suarez, David L. Boschmans, Marc Ozhelvaci, Orkun Marche, S. van den Berg, Thierry P. TI Rapid Detection of Eurasian and American H7 Subtype Influenza A Viruses Using a Single TaqManMGB Real-Time RT-PCR SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; real-time RT-PCR; molecular diagnosis; H7 subtype ID PATHOGENIC AVIAN INFLUENZA; GENE SEGMENT REASSORTMENT; BRITISH-COLUMBIA; PHYLOGENETIC ANALYSIS; HUMAN ILLNESS; HUMAN-BEINGS; OUTBREAK; H5; CONJUNCTIVITIS; ASSAY AB A real-time reverse transcription PCR (RRT-PCR) targeting a highly conserved HA2 H7 region was developed for the detection of all H7 subtype avian influenza viruses (PanH7). The wide phylogenetic scope and analytical sensitivity and specificity were validated with the use of a panel of 56 diverse influenza A viruses. The detection limit was determined with the use of serial dilutions of Eurasian isolates A/Ck/BE/06775/2003 and A/Ck/It/1067/v99 and North American isolates A/CK/PA/143586/2001 and A/Quail/PA/20304/1998, to be 1 log(10) higher than the detection limit of the generic influenza A matrix RRT-PCR (about 2.5 EID(50)/reaction compared to 0.25 EID(50)/reaction for matrix). Diagnostic test properties of PanH7 were determined with the use of 102 swabs from A/Ck/It/1067/v99 experimentally infected chickens, and were not affected by the increased detection limit of PanH7. In comparison to matrix RRT-PCR and virus isolation in embryonated chicken eggs (VI), the PanH7 detected more weakly positive oropharyngeal swabs at the onset of the infection. PanH7 diagnostic sensitivity compared to virus isolation (VI) was 83.3% (compared to 72.2% for matrix RRT-PCR); and diagnostic specificity was 88.1% (94.0% for matrix). The PanH7 test can also be tailored to detect only American (AmH7) or only Eurasian (EurH7) strains by changing the mix of forward and reverse primers used in combination with the unique probe. Overall, this new test is a valuable tool for the detection and identification of H7 subtype influenza A. C1 [Van Borm, Steven; Boschmans, Marc; Ozhelvaci, Orkun; Marche, S.; van den Berg, Thierry P.] Vet & Agrochem Res Ctr, B-1180 Brussels, Belgium. [Suarez, David L.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Van Borm, S (reprint author), Vet & Agrochem Res Ctr, 99 Groeselenbergstr, B-1180 Brussels, Belgium. EM stevenvanborm@yar.fgov.be RI Van Borm, Steven/P-3336-2014 NR 46 TC 3 Z9 3 U1 0 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 632 EP 638 DI 10.1637/8734-032509-ResNote.1 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300080 PM 20521706 ER PT J AU Pedersen, J Killian, ML Hines, N Senne, D Panigrahy, B Ip, HS Spackman, E AF Pedersen, Janice Killian, Mary Lea Hines, Nichole Senne, Dennis Panigrahy, Brundaban Ip, Hon S. Spackman, Erica TI Validation of a Real-Time Reverse Transcriptase-PCR Assay for the Detection of H7 Avian Influenza Virus SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 7th International Symposium on Avian Influenza CY APR 05-08, 2009 CL Univ Georgia, Athens, GA HO Univ Georgia DE avian influenza; poultry; real-time RT-PCR; surveillance; wild birds ID CANADA AB This report describes the validation of an avian influenza virus (AIV) H7 subtype-specific real-time reverse transcriptase PCR (rRT-PCR) assay developed at the Southeast Poultry Research Laboratory (SEPRL) for the detection of H7 AI in North and South American wild aquatic birds and poultry. The validation was a collaborative effort by the SEPRL and the National Veterinary Services Laboratories. The 2008 H7 rRT-PCR assay detects 10(1) 50% embryo infectious doses per reaction, or 10(3)-10(4) copies of transcribed H7 RNA. Diagnostic sensitivity and specificity were estimated to be 97.5% and 82.4%, respectively; the assay was shown to be specific for H7 AI when tested with >270 wild birds and poultry viruses. Following validation, the 2008 rRT-PCR. procedure was adopted as an official U.S. Department of Agriculture procedure for the detection of H7 AIV. The 2008 H7 assay replaced the previously used (2002) assay, which does not detect H7 viruses currently circulating in wild birds in North and South America. C1 [Pedersen, Janice; Killian, Mary Lea; Hines, Nichole; Senne, Dennis; Panigrahy, Brundaban] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA. [Ip, Hon S.] US Geol Survey, US Dept Interior, Ctr Hlth, Madison, WI 53711 USA. [Spackman, Erica] ARS, USDA, Res Lab, Athens, GA 30605 USA. RP Pedersen, J (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA. EM Janice.C.Pedersen@aphis.usda.gov NR 9 TC 1 Z9 3 U1 0 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2010 VL 54 IS 1 SU S BP 639 EP 643 DI 10.1637/8911-043009-Reg.1 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 580NF UT WOS:000276455300081 PM 20521707 ER PT J AU Casler, MD AF Casler, Michael D. TI DOE Bioenergy Center Special Issue: The Great Lakes Bioenergy Research Center (GLBRC) SO BIOENERGY RESEARCH LA English DT Editorial Material DE Bioenergy centers; US Department of Energy C1 ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Casler, MD (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM michael.casler@ars.usda.gov NR 0 TC 2 Z9 2 U1 0 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2010 VL 3 IS 1 SI SI BP 1 EP 2 DI 10.1007/s12155-009-9076-4 PG 2 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 607XU UT WOS:000278541400001 ER PT J AU Casler, MD AF Casler, Michael D. TI Changes in Mean and Genetic Variance During Two Cycles of Within- family Selection in Switchgrass SO BIOENERGY RESEARCH LA English DT Article DE Panicum virgatum L; Biomass yield; Breeding; Biofuel crops ID FORAGE GRASS TRIALS; BIOMASS YIELD; POPULATIONS; SEASON; ADAPTATION; HETEROSIS; FREQUENCY; SURVIVAL; PLANTS AB Switchgrass (Panicum virgatum L.) is a candidate for cellulosic bioenergy feedstock development. Because biomass yield is the most important biological factor limiting the commercial development and deployment of switchgrass as a cellulosic bioenergy feedstock efforts must be undertaken to develop improved cultivars. The objectives of this study were (1) to conduct two cycles of within-family selection for increased biomass yield in WS4U switchgrass and (2) to simultaneously evaluate progress from selection relative to the mean of the original WS4U population. Each of the 150 WS4U families was subjected to phenotypic selection for vigor, seed production, and disease resistance. The mean of all families increased relative to the original WS4U population by 0.36 Mg ha(-1) cycle(-1) for biomass yield and 3.0% cycle-1 for ground cover. Gains were uniform across two diverse evaluation locations, indicating that selection gains were robust relative to some variation in Hardiness Zone and soil type. Two cycles of within-family selection led to a homogenization of the diverse families, creating novel recombinations and reducing the family genetic variance to near zero. It is hypothesized that selection and recombination has led to replication of favorable alleles across pedigrees with differing genetic backgrounds, increasing the likelihood of including these favorable alleles in the progeny of future selections. The rate of genetic progress is expected to increase in future cycles of selection with a combination of within-family phenotypic selection and half-sib progeny testing of selected families. C1 ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Casler, MD (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM michael.casler@ars.usda.gov FU USDA-ARS CRIS [3655-41000-003-00D, 365541000-004-00D]; University of Wisconsin Agricultural Research Stations; U.S. Department of Energy Great Lakes Bioenergy Research Center [DE-FC02-07ER64494] FX I thank Andy Beal, Doug Foy, and Nick Baker for assistance with various aspects of this experiment and Mike Bertram of the Marshfield Agricultural Research Station for providing land and field-management support. This research was funded in part by USDA-ARS CRIS Project Nos. 3655-41000-003-00D and 365541000-004-00D, the University of Wisconsin Agricultural Research Stations, and the U.S. Department of Energy Great Lakes Bioenergy Research Center Grant DE-FC02-07ER64494. NR 24 TC 21 Z9 21 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2010 VL 3 IS 1 SI SI BP 47 EP 54 DI 10.1007/s12155-009-9071-9 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 607XU UT WOS:000278541400007 ER PT J AU Taheripour, F Hertel, TW Tyner, WE Beckman, JF Birur, DK AF Taheripour, Farzad Hertel, Thomas W. Tyner, Wallace E. Beckman, Jayson F. Birur, Dileep K. TI Biofuels and their by-products: Global economic and environmental implications SO BIOMASS & BIOENERGY LA English DT Article DE General equilibrium; Biofuel; Mandates; Land use changes; DDGS ID POLICY AB Recently a number of papers have used general equilibrium models to study the economy-wide and environmental consequences of the first generation of biofuels (FGB). In this paper, we argue that nearly all of these studies have overstated the impacts of FGB on global agricultural and land markets due to the fact that they have ignored the role of biofuel by-products. Feed by-products of FGB, such as dried distillers grains with solubles (DDGS) and oilseed meals (VOBP), are used in the livestock industry as protein and energy sources. Their presence mitigates the price impacts of biofuel production. More importantly, they reduce the demand for cropland and moderate the indirect land use consequences of FGB. This paper explicitly introduces DDGS and VOBP into a global computational general equilibrium (CGE) model, developed at the Center for Global Trade Analysis at Purdue University, to examine the economic and environmental impacts of regional and international mandate policies designed to stimulate bioenergy production and use. We show that models with and without by-products reveal different portraits of the economic impacts of the US and EU biofuel mandates for the world economy in 2015. While both models demonstrate significant changes in the agricultural production pattern across the world, the model with by-products shows smaller changes in the production of cereal grains and larger changes for oilseeds products in the US and EU, and the reverse for Brazil. Models that omit by-products are found to overstate cropland conversion from US and EU mandates by about 27%. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Taheripour, Farzad; Hertel, Thomas W.; Tyner, Wallace E.; Beckman, Jayson F.; Birur, Dileep K.] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. [Beckman, Jayson F.] USDA, Washington, DC USA. RP Taheripour, F (reprint author), Purdue Univ, Dept Agr Econ, 403 W State St, W Lafayette, IN 47907 USA. EM tfarzad@purdue.edu NR 16 TC 70 Z9 72 U1 3 U2 42 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD MAR PY 2010 VL 34 IS 3 BP 278 EP 289 DI 10.1016/j.biombioe.2009.10.017 PG 12 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 576UE UT WOS:000276173800004 ER PT J AU Lovanh, N Warren, J Sistani, K AF Lovanh, Nanh Warren, Jason Sistani, Karamat TI Determination of ammonia and greenhouse gas emissions from land application of swine slurry: A comparison of three application methods SO BIORESOURCE TECHNOLOGY LA English DT Article DE Ammonia; Gas fluxes; Greenhouse gases; Land application; Swine manure ID NITROUS-OXIDE EMISSIONS; METHANE EMISSIONS; PIG SLURRY; FLUXES; SOILS; FIELD; VOLATILIZATION; FERTILIZER; INJECTION; EFFLUENT AB In this study, the comparison and monitoring of the initial greenhouse gas (GHG) emissions using a flux chamber and gas analyzer from three different liquid manure application methods at a swine farm in Kentucky were carried out. Swine slurry was applied to farmland by row injection, surface spray, and Aerway injection. Ammonia and GHG concentrations were monitored immediately after application, 72 and 216 h after application. The results showed that the initial ammonia flux ranged from 5.80 mg m(-2) h(-1) for the surface spray method to 1.80 mg m(-2) h(-1) for the row injection method. The initial fluxes of methane ranged from 8.75 mg m-2 h(-1) for surface spray to 2.27 mg m(-2) h(-1) for Aerway injection, carbon dioxide ranged from 4357 mg m(-2) h(-1) for surface spray to 60 mg m(-2) h(-1) for row injection, and nitrous oxide ranged from 0.89 mg m(-2) h(-1) for surface spray to 0.22 mg m(-2) h(-1) for row injection. However, the Aerway injection method seemed to create the highest gas (GHG) concentrations inside the monitoring chambers at the initial application and produced the highest gas fluxes at subsequent sampling time (e.g., 72 h after application). Nevertheless, the surface spray method appeared to produce the highest gas fluxes, and the row injection method appeared to emit the least amount of greenhouse gases into the atmosphere. Gas fluxes decreased over time and did not depend on the initial head-space concentration in the monitoring flux chambers. Published by Elsevier Ltd. C1 [Lovanh, Nanh; Warren, Jason; Sistani, Karamat] USDA ARS, Anim Waste Management Res Unit, Bowling Green, KY 42104 USA. RP Lovanh, N (reprint author), USDA ARS, Anim Waste Management Res Unit, 230 Bennett Lane, Bowling Green, KY 42104 USA. EM nanh.lovanh@ars.usda.gov NR 28 TC 14 Z9 14 U1 2 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD MAR PY 2010 VL 101 IS 6 BP 1662 EP 1667 DI 10.1016/j.biortech.2009.09.078 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 552ES UT WOS:000274271400014 PM 19854045 ER PT J AU Stone, KC Hunt, PG Cantrell, KB Ro, KS AF Stone, K. C. Hunt, P. G. Cantrell, K. B. Ro, K. S. TI The potential impacts of biomass feedstock production on water resource availability SO BIORESOURCE TECHNOLOGY LA English DT Article DE Water; Water scarcity; Water availability; Climate change; Bioenergy ID WESTERN UNITED-STATES; SUBSURFACE DRIP IRRIGATION; SWINE WASTE-WATER; TABLE MANAGEMENT; SWEET SORGHUM; ETHANOL-PRODUCTION; SOIL PRODUCTIVITY; ENERGY-PRODUCTION; RESIDUE REMOVAL; CLIMATE-CHANGE AB Biofuels are a major topic of global interest and technology development. Whereas bioenergy crop production is highly dependent on water, bioenergy, development requires effective allocation and management of water. The objectives of this investigation were to assess the bioenergy production relative to the impacts on water resource related factors: (1) climate and weather impact on water supplies for biomass production: (2) water use for major bioenergy crop production; and (3) potential alternatives to improve water supplies for bioenergy. Shifts to alternative bioenergy crops with greater water demand may produce unintended consequences for both water resources and energy feedstocks. Sugarcane and corn require 458 and 2036 m(3) water/m(3) ethanol produced, respectively. The water requirements for corn grain production to meet the US-DOE Billion-Ton Vision may increase approximately 6-fold from 8.6 to 50.1 km(3). Furthermore, climate change is impacting water resources throughout the world. In the western US, runoff from snowmelt is occurring earlier altering the timing of water availability. Weather extremes, both drought and flooding, have occurred more frequently over the last 30 years than the previous 100 years. All of these weather events impact bioenergy crop production. These events may be partially mitigated by alternative water management systems that offer potential for more effective water use and conservation. A few potential alternatives include controlled drainage and new next-generation livestock waste treatment systems. Controlled drainage can increase water available to plants and simultaneously improve water quality. New livestock waste treatments systems offer the potential to utilize treated wastewater to produce bioenergy crops. New technologies for cellulosic biomass conversion via thermochemical conversion offer the potential for using more diverse feedstocks with dramatically reduced water requirements. The development of bioenergy feedstocks in the US and throughout the world should carefully consider water resource limitations and their critical connections to ecosystem integrity and sustainability of human food. Published by Elsevier Ltd. C1 [Stone, K. C.; Hunt, P. G.; Cantrell, K. B.; Ro, K. S.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Stone, KC (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St Florence, Florence, SC 29501 USA. EM ken.stone@ars.usda.gov RI Langholtz, Matthew/B-9416-2012 OI Langholtz, Matthew/0000-0002-8153-7154 NR 91 TC 47 Z9 48 U1 4 U2 44 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD MAR PY 2010 VL 101 IS 6 BP 2014 EP 2025 DI 10.1016/j.biortech.2009.10.037 PG 12 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 552ES UT WOS:000274271400066 PM 19939667 ER PT J AU Beechie, TJ Sear, DA Olden, JD Pess, GR Buffington, JM Moir, H Roni, P Pollock, MM AF Beechie, Timothy J. Sear, David A. Olden, Julian D. Pess, George R. Buffington, John M. Moir, Hamish Roni, Philip Pollock, Michael M. TI Process-based Principles for Restoring River Ecosystems SO BIOSCIENCE LA English DT Article DE river restoration; ecosystem dynamics; ecosystem processes ID MODIFYING DAM OPERATIONS; ECOLOGICAL RESPONSES; GENERAL PROTOCOL; CHANNEL NETWORKS; FRESH-WATER; RESTORATION; STREAM; CONNECTIVITY; SEDIMENT; DYNAMICS AB Process-based restoration aims to reestablish normative rates and magnitudes of physical, chemical, and biological processes that sustain river and floodplain ecosystems. Ecosystem conditions at any site are governed by hierarchical regional, watershed, and reach-scale processes controlling hydrologic and sediment regimes; floodplain and aquatic habitat dynamics; and riparian and aquatic biota. We outline and illustrate four process-based principles that ensure river restoration will be guided toward sustainable actions: (1) restoration actions should address the root causes of degradation, (2) actions must be consistent with the physical and biological potential of the site, (3) actions should be at a scale commensurate with environmental problems, and (4) actions should have clearly articulated expected outcomes for ecosystem dynamics. Applying these principles will help avoid common pitfalls in river restoration, such as creating habitat types that are outside of a site's natural potential, attempting to build static habitats in dynamic environments, or constructing habitat features that are ultimately overwhelmed by unconsidered system drivers. C1 [Beechie, Timothy J.; Pess, George R.; Pollock, Michael M.] NOAA, Fisheries NW Fisheries Sci Ctr, Seattle, WA USA. [Sear, David A.] Univ Southampton, Sch Geog, Southampton SO9 5NH, Hants, England. [Olden, Julian D.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Buffington, John M.] US Forest Serv, Rocky Mt Res Stn, Boise, ID USA. [Moir, Hamish] Macaulay Inst, Aberdeen, Scotland. RP Beechie, TJ (reprint author), NOAA, Fisheries NW Fisheries Sci Ctr, Seattle, WA USA. EM tim.beechie@noaa.gov RI Olden, Julian/A-8535-2010; Sear, David/J-6333-2012; OI Olden, Julian/0000-0003-2143-1187 NR 67 TC 158 Z9 163 U1 17 U2 147 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD MAR PY 2010 VL 60 IS 3 BP 209 EP 222 DI 10.1525/bio.2010.60.3.7 PG 14 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 571CW UT WOS:000275729900007 ER PT J AU Wang, S Tiwari, G Jiao, S Johnson, JA Tang, J AF Wang, S. Tiwari, G. Jiao, S. Johnson, J. A. Tang, J. TI Developing postharvest disinfestation treatments for legumes using radio frequency energy SO BIOSYSTEMS ENGINEERING LA English DT Article ID IN-SHELL WALNUTS; TRIBOLIUM-CASTANEUM COLEOPTERA; THERMAL DEATH KINETICS; CONTROL CODLING MOTH; HEATING UNIFORMITY; INSECT CONTROL; ELEVATED-TEMPERATURES; MICROWAVE-ENERGY; PRODUCT QUALITY; MORTALITY AB There is an urgent need to develop technically effective and environmentally sound phytosanitary and quarantine treatments for the legume industry to replace chemical fumigation. The goal of this study was to develop practical non-chemical treatments for postharvest disinfestations of legumes using radio frequency (RF) energy. A pilot-scale 27 MHz, 6 kW RF unit was used to investigate RF heating and consequent quality attributes in treated chickpea, green pea, and lentil samples. Only 5-7 min were needed to raise the central temperature of 3 kg legume samples to 60 degrees C using RF energy, compared to more than 275 mm when using forced hot air at 60 degrees C. RF heating uniformity in legume samples was improved by adding forced hot air, and back and forth movements on the conveyor at 0.56 m min(-1). The final temperatures exceeded 55.8 degrees C in the interior of the sample container and 57.3 degrees C on the surface for all three legumes, resulting in low uniformity index values of 0.014-0.016 (ratio of standard deviation to the average temperature rise) for the interior temperature distributions and 0.061-0.078 for surface temperature distributions. RF treatments combined with forced hot air at 60 degrees C to maintain the target treatment temperature for 10 min followed by forced room air cooling through a 1 cm product layer provided good product quality. No significant differences in weight loss, moisture content, colour or germination were observed between RF treatments and unheated controls. (C) 2009 IAgrE. Published by Elsevier Ltd. All rights reserved. C1 [Wang, S.; Tiwari, G.; Jiao, S.; Tang, J.] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Johnson, J. A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Wang, S (reprint author), Washington State Univ, Dept Biol Syst Engn, 213 LJ Smith Hall, Pullman, WA 99164 USA. EM shaojin_wang@wsu.edu RI Wang, Shaojin/C-4968-2012; Tang, Juming/A-4027-2009 OI Tang, Juming/0000-0001-9449-1004 FU USDA-CSREES [2008-34103-19091]; Washington State University Agricultural Research Center FX This research was supported by grants from USDA-CSREES (2008-34103-19091), and Washington State University Agricultural Research Center. We thank Drs. Zhongli Pan (USDA-WRRC, Albany, CA) and Mark Cascade (USDA-ARS, Manhattan, KS) for reviewing this manuscript and providing constructive suggestions. NR 44 TC 58 Z9 61 U1 4 U2 30 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 J9 BIOSYST ENG JI Biosyst. Eng. PD MAR PY 2010 VL 105 IS 3 BP 341 EP 349 DI 10.1016/j.biosystemseng.2009.12.003 PG 9 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA 576CK UT WOS:000276121200008 ER PT J AU Widmer, W AF Widmer, Wilbur TI An improved method for analysis of biomass sugars and galacturonic acid by anion exchange chromatography SO BIOTECHNOLOGY LETTERS LA English DT Article DE Biomass; Cellobiose; Citrus; Ethanol; Sugars ID PEEL AB The most accurate analysis method for sugars in biomass, based on gas chromatography, requires a time consuming and laborious sample derivatation to trimethylsilanes or alditol acetates. In comparison, sample preparations for sugar analysis by liquid chromatography are simple water dilutions. However, HPLC methods either require long analysis times, use of expensive solvents, or do not give good resolution of sugars. A gradient method developed previously using a Dionex PA-1 column and pulsed-amperometric detection was modified to reduce analysis time from 75 to less than 40 min and provide good resolution of arabinose, rhamnose, galactose, xylose, glucose, fructose, sucrose, cellobiose, and galacturonic acid in both standards and hydrolyzed citrus waste biomass. C1 ARS, USDA, Citrus & Subtrop Prod Res Lab, Winter Haven, FL 33881 USA. RP Widmer, W (reprint author), ARS, USDA, Citrus & Subtrop Prod Res Lab, 600 Ave S NW, Winter Haven, FL 33881 USA. EM Wilbur.widmer@ars.usda.gov NR 7 TC 2 Z9 2 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD MAR PY 2010 VL 32 IS 3 BP 435 EP 438 DI 10.1007/s10529-009-0162-7 PG 4 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 555UW UT WOS:000274542600016 PM 19898748 ER PT J AU Wasilenko, JL Sarmento, L Spatz, S Pantin-Jackwood, M AF Wasilenko, Jamie L. Sarmento, Luciana Spatz, Stephen Pantin-Jackwood, Mary TI Cell Surface Display of Highly Pathogenic Avian Influenza Virus Hemagglutinin on the Surface of Pichia pastoris Cells Using alpha-Agglutinin for Production of Oral Vaccines SO BIOTECHNOLOGY PROGRESS LA English DT Article DE Pichia pastoris; cell surface display; hemagglutinin; alpha-agglutinin; H5N1; avian influenza ID YEAST; EXPRESSION; PROTEIN; SYSTEM; CONSTRUCTION; GENE AB Yeast is an ideal organism to express viral antigens because yeast glycosylate proteins more similarly to mammals than bacteria. Expression of proteins in yeast is relatively fast and inexpensive. In addition to the convenience of production, for purposes of vaccination, yeast has been shown to have natural adjuvant activity making the expressed proteins more immunogenic when administered along with yeast cell wall components. Development of "genetic systems to display foreign proteins on the surface of yeast via fusion to glycosylphos-phatidylinositol-anchored (GPI) proteins has further simplified the purification of recombinant proteins by not requiring harsh treatments for cellular lysis or protein purification. We have expressed the hemagglutinin protein from a highly pathogenic avian influenza (HPAI) virus [A/Egret/HK/757.2/02], subtype H5N1, on the surface of the yeast strain Pichia pastoris, as an anchored C-terminal fusion with the Saccharomyces cerevisiae GPI-anchored cell wall protein, alpha-agglutinin. Surface expression of the hemagglutinin fusion protein was demonstrated by immunofluorescence microscopy. Functionally, the fusion protein retained hemagglutinin agglutinating activity, and oral vaccination with the yeast resulted in production of virus neutralizing antibodies. This study represents the first steps in the generation of a yeast-based vaccine for protection against highly pathogenic strains of avian influenza. Published 2009 American Institute of Chemical Engineers(dagger) Biotechnol. Prog., 26: 542-547, 2010 C1 [Wasilenko, Jamie L.; Sarmento, Luciana; Spatz, Stephen; Pantin-Jackwood, Mary] USDA, SE Poultry Res Lab, ARS, Athens, GA 30605 USA. RP Pantin-Jackwood, M (reprint author), USDA, SE Poultry Res Lab, ARS, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ARS.USDA.gov FU United States Department of Agriculture [6612-32000-053] FX The authors thank Diane Smith for excellent technical support. This work was funded by United States Department of Agriculture CRIS# 6612-32000-053. NR 20 TC 17 Z9 17 U1 1 U2 8 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 8756-7938 J9 BIOTECHNOL PROGR JI Biotechnol. Prog. PD MAR-APR PY 2010 VL 26 IS 2 BP 542 EP 547 DI 10.1002/btpr.343 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 587IV UT WOS:000276985400026 PM 19950135 ER PT J AU Tucker, KL Mattei, J Noel, SE Collado, BM Mendez, J Nelson, J Griffith, J Ordovas, JM Falcon, LM AF Tucker, Katherine L. Mattei, Josiemer Noel, Sabrina E. Collado, Bridgette M. Mendez, Jackie Nelson, Jason Griffith, John Ordovas, Jose M. Falcon, Luis M. TI The Boston Puerto Rican Health Study, a longitudinal cohort study on health disparities in Puerto Rican adults: challenges and opportunities SO BMC PUBLIC HEALTH LA English DT Article ID NUTRITION EXAMINATION SURVEY; NON-HISPANIC WHITE; FOOD FREQUENCY QUESTIONNAIRE; IMPAIRED GLUCOSE-TOLERANCE; STRESS SCALE PSS; UNITED-STATES; PHYSICAL-ACTIVITY; PERCEIVED STRESS; NATIONAL-HEALTH; US ADULTS AB Background: The Boston Puerto Rican Health Study is an ongoing longitudinal cohort study designed to examine the role of psychosocial stress on presence and development of allostatic load and health outcomes in Puerto Ricans, and potential modification by nutritional status, genetic variation, and social support. Methods: Self-identified Puerto Ricans, aged 45-75 years and residing in the Boston, MA metro area, were recruited through door-to-door enumeration and community approaches. Participants completed a comprehensive set of questionnaires and tests. Blood, urine and salivary samples were extracted for biomarker and genetic analysis. Measurements are repeated at a two-year follow-up. Results: A total of 1500 eligible participants completed baseline measurements, with nearly 80% two-year follow-up retention. The majority of the cohort is female (70%), and many have less than 8(th) grade education (48%), and fall below the poverty level (59%). Baseline prevalence of health conditions is high for this age range: considerable physical (26%) and cognitive (7%) impairment, obesity (57%), type 2 diabetes (40%), hypertension (69%), arthritis (50%) and depressive symptomatology (60%). Conclusions: The enrollment of minority groups presents unique challenges. This report highlights approaches to working with difficult to reach populations, and describes some of the health issues and needs of Puerto Rican older adults. These results may inform future studies and interventions aiming to improve the health of this and similar communities. C1 [Tucker, Katherine L.; Mattei, Josiemer; Noel, Sabrina E.; Collado, Bridgette M.; Mendez, Jackie; Ordovas, Jose M.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tucker, Katherine L.; Falcon, Luis M.] Northeastern Univ, Boston, MA 02115 USA. [Mattei, Josiemer; Noel, Sabrina E.; Ordovas, Jose M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Nelson, Jason; Griffith, John] Tufts Med Ctr, Boston, MA USA. RP Tucker, KL (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM kl.tucker@neu.edu RI Tucker, Katherine/A-4545-2010; Falcon, Luis/C-1237-2009; Mattei, Josiemer/H-1800-2016; OI Mattei, Josiemer/0000-0001-5424-8245; Tucker, Katherine/0000-0001-7640-662X; Ordovas, Jose/0000-0002-7581-5680 FU National Institutes of Health-National Institute on Aging [P01-AG023394]; United States Department of Agriculture, Agriculture Research Institute [58-1950-7-707] FX This study is supported by the National Institutes of Health-National Institute on Aging grant number P01-AG023394, and by the United States Department of Agriculture, Agriculture Research Institute agreement number 58-1950-7-707. NR 73 TC 66 Z9 67 U1 6 U2 16 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD MAR 1 PY 2010 VL 10 AR 107 DI 10.1186/1471-2458-10-107 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 580KP UT WOS:000276447400001 PM 20193082 ER PT J AU Baxter, I AF Baxter, Ivan TI Ionomics: The functional genomics of elements SO BRIEFINGS IN FUNCTIONAL GENOMICS LA English DT Article DE ionomics; maize; Arabidopsis; mineral nutrition ID ARABIDOPSIS-THALIANA; LOTUS-JAPONICUS; HOMEOSTASIS; PLANTS; NUTRIENT; SYSTEM AB Ionomics is the study of elemental accumulation in living systems using high-throughput elemental profiling. This approach has been applied extensively in plants for forward and reverse genetics, screening diversity panels, and modeling of physiological states. In this review, I will discuss some of the advantages and limitations of the ionomics approach as well as the important parameters to consider when designing ionomics experiments, and how to evaluate ionomics data. C1 USDA ARS Plant Genet, Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. RP Baxter, I (reprint author), USDA ARS Plant Genet, Res Unit, Donald Danforth Plant Sci Ctr, 975 N Warson Rd, St Louis, MO 63132 USA. EM ivan.baxter@ars.usda.gov RI Baxter, Ivan/A-1052-2009 OI Baxter, Ivan/0000-0001-6680-1722 NR 17 TC 31 Z9 32 U1 1 U2 19 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 2041-2649 J9 BRIEF FUNCT GENOMICS JI Brief. Funct. Genomics PD MAR PY 2010 VL 9 IS 2 BP 149 EP 156 DI 10.1093/bfgp/elp055 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 577AG UT WOS:000276191200008 PM 20081216 ER PT J AU Jannink, JL Lorenz, AJ Iwata, H AF Jannink, Jean-Luc Lorenz, Aaron J. Iwata, Hiroyoshi TI Genomic selection in plant breeding: from theory to practice SO BRIEFINGS IN FUNCTIONAL GENOMICS LA English DT Article DE breeding value prediction; marker-assisted selection; linkage disequilibrium; ridge regression; machine learning ID MARKER-ASSISTED SELECTION; GENOMEWIDE SELECTION; QUANTITATIVE TRAITS; MOLECULAR MARKERS; RIDGE-REGRESSION; COMPLEX TRAITS; WIDE SELECTION; DAIRY-CATTLE; MAIZE; ACCURACY AB We intuitively believe that the dramatic drop in the cost of DNA marker information we have experienced should have immediate benefits in accelerating the delivery of crop varieties with improved yield, quality and biotic and abiotic stress tolerance. But these traits are complex and affected by many genes, each with small effect. Traditional marker-assisted selection has been ineffective for such traits. The introduction of genomic selection (GS), however, has shifted that paradigm. Rather than seeking to identify individual loci significantly associated with a trait, GS uses all marker data as predictors of performance and consequently delivers more accurate predictions. Selection can be based on GS predictions, potentially leading to more rapid and lower cost gains from breeding. The objectives of this article are to review essential aspects of GS and summarize the important take-home messages from recent theoretical, simulation and empirical studies. We then look forward and consider research needs surrounding methodological questions and the implications of GS for long-term selection. C1 [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Lorenz, Aaron J.] USDA, Barley Coordinated Agr Project, Washington, DC USA. [Iwata, Hiroyoshi] Natl Agr Res Ctr, Natl Agr & Food Sci Res Org, Tokyo, Japan. RP Jannink, JL (reprint author), Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM jeanluc.jannink@ars.usda.gov FU United States Department of Agriculture, National Institute of Food and Agriculture [2009-85606-05701, 200965300-05661] FX This work was supported by the United States Department of Agriculture, National Institute of Food and Agriculture (2009-85606-05701, 200965300-05661). NR 74 TC 301 Z9 308 U1 5 U2 113 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 2041-2649 J9 BRIEF FUNCT GENOMICS JI Brief. Funct. Genomics PD MAR PY 2010 VL 9 IS 2 BP 166 EP 177 DI 10.1093/bfgp/elq001 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 577AG UT WOS:000276191200010 PM 20156985 ER PT J AU Landolt, PJ Lowery, DT Wright, LC Smithhisler, C Guedot, C James, DG AF Landolt, Peter J. Lowery, D. Thomas Wright, Lawrence C. Smithhisler, Constance Guedot, Christelle James, David G. TI Trap response of cutworm moths (Abagrotis orbis) to a sex-attractant lure in grape vineyards SO CANADIAN ENTOMOLOGIST LA English DT Article ID LEPIDOPTERA-NOCTUIDAE; TRICHOPLUSIA-NI; PSEUDOPLUSIA-INCLUDENS; REPRODUCTIVE ISOLATION; PHEROMONE COMPONENTS; IDENTIFICATION; ARMYWORM AB Larvae of Abagrotis orbis (Grote) (Lepidoptera. Noctuidae) are climbing cutworms and can damage grapevines, Vitis vinifera L (Vitaceac), in early spring by consuming expanding buds. A sex attractant would be useful for monitoring this insect in commercial vineyards (Z)-7-Tetradecenyl acetate and (Z)-11-hexadecenyl acetate were found in extracts of female abdominal tips. In multiple field experiments, male A orbis were captured in traps baited with a combination of these two chemicals but not in traps baited with either chemical alone. Males were trapped from mid-September to early October in south-central Washington and south-central British Columbia. Other noctuid moths (Mamestra configurata Walker, Xestia c-nigrum (L), and Feltia jaculifera (Guenee)) were also captured in traps baited with the A. this pheromone and may complicate the use of this lure to monitor A. orbis. Abagrotis discoidalis (Grote) was captured in traps baited with (Z)-7-tetradecenyl acetate but not in traps baited with the two chemicals together. C1 [Landolt, Peter J.; Smithhisler, Constance; Guedot, Christelle] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Lowery, D. Thomas] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada. [Wright, Lawrence C.; James, David G.] Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Landolt, PJ (reprint author), ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. FU British Columbia Wine Grape Council; Agriculture and Agri-Food Canada through the Matching Investment Initiative; Washington State Concord Grape Research Council; Washington Wine Advisory Council FX Technical assistance was provided by Keith DeGlow, Daryl Green, Linda Jensen, and Stephanie Jesperson We thank Gary Judd for advice and support for this study and Lars Crabo and Don Lafontaine for confirmation of some moth identifications. This work was supported in part by funding from the British Columbia Wine Grape Council, Agriculture and Agri-Food Canada through the Matching Investment Initiative, the Washington State Concord Grape Research Council, and the Washington Wine Advisory Council. Juan Uribe of Ste Michelle Wine Estates, Bill Powers of Badger Mountain Vineyards, and Jason Don of Wyckoff Farms allowed us to use their vineyards. NR 29 TC 0 Z9 0 U1 1 U2 6 PU ENTOMOL SOC CANADA PI OTTAWA PA 393 WINSTON AVE, OTTAWA, ONTARIO K2A 1Y8, CANADA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD MAR-APR PY 2010 VL 142 IS 2 BP 135 EP 142 DI 10.4039/n09-063 PG 8 WC Entomology SC Entomology GA 589XS UT WOS:000277188100004 ER PT J AU Cooper, WR Puterka, GJ Glenn, DM AF Cooper, W. R. Puterka, G. J. Glenn, D. M. TI Relative attractiveness of colour traps to pear psylla in relation to seasonal changes in pear phenology SO CANADIAN ENTOMOLOGIST LA English DT Article ID HOMOPTERA; INSECTS; APHIDS AB Monitoring of pear psylla (Cacopsylla pyricola (Forster). Hemiptera: Psyllidae) prior to spring bud break could aid in predicting the size of subsequent spring populations and lead to improved proactive management decisions. Yellow traps are commonly used to monitor hemipteran pests including pear psylla, but very little is known regarding seasonal changes in attractiveness of yellow traps or relative attractiveness of colours other than yellow This study presents seasonal colour-trap preferences of pear psyllas based on pear (Pyrus communis L. Rosaceae) phenological stages in the mid-Atlantic region of the United States of America. Black, blue, brown, clear (colourless), green, orange, red, white, and yellow traps were assayed against wild adult psylla populations over a 2-year period Pear psyllas had a strong preference for yellow and orange when green leaves were present: however, we found no statistically significant difference between traps of different colours prior to spring bud break Significantly more female psyllas were caught overall, but there was no gender-based colour bias None of our colour traps caught significantly fewer psyllas than did clear (background hue) traps, suggesting that no traps were repellent C1 [Cooper, W. R.] ARS, USDA, Western Integrated Cropping Syst Res Unit, Shafter, CA 93263 USA. [Puterka, G. J.] ARS, USDA, Wheat Peanuts & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Glenn, D. M.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Cooper, WR (reprint author), ARS, USDA, Western Integrated Cropping Syst Res Unit, 17053 N Shafter Ave, Shafter, CA 93263 USA. RI Cooper, William/D-3205-2017 FU Appalachian Fruit Research Station FX We acknowledge research technicians of the United States Department of Agriculture, Agricultural Research Service, for technical assistance with aspects of various experiments, and the Appalachian Fruit Research Station for research funding and space NR 11 TC 1 Z9 1 U1 0 U2 7 PU ENTOMOL SOC CANADA PI OTTAWA PA 393 WINSTON AVE, OTTAWA, ONTARIO K2A 1Y8, CANADA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD MAR-APR PY 2010 VL 142 IS 2 BP 188 EP 191 DI 10.4039/n09-018 PG 4 WC Entomology SC Entomology GA 589XS UT WOS:000277188100009 ER PT J AU Bradford, JB Kastendick, DN AF Bradford, John B. Kastendick, Douglas N. TI Age-related patterns of forest complexity and carbon storage in pine and aspen-birch ecosystems of northern Minnesota, USA SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID COARSE WOODY DEBRIS; PLANAR INTERSECT METHOD; TEMPERATE FORESTS; BRITISH-COLUMBIA; CLIMATE-CHANGE; UNITED-STATES; MANAGEMENT; DYNAMICS; CHRONOSEQUENCES; SEQUESTRATION AB Forest managers are seeking strategies to create stands that can adapt to new climatic conditions and simultaneously help mitigate increases in atmospheric CO(2). Adaptation strategies often focus on enhancing resilience by maximizing forest complexity in terms of species composition and size structure, while mitigation involves sustaining carbon storage and sequestration. Altered stand age is a fundamental consequence of forest management. and stand age is a powerful predictor of ecosystem structure and function in even-aged stands. However, the relationship between stand age and either complexity or carbon storage and sequestration, especially trade-offs between the two, are not well characterized. We quantified these relationships in clearcut-origin, unmanaged pine and aspen chronosequences ranging from <10 to >130 years in northern Minnesota. Complexity generally increased with age, although compositional complexity changed more over time in aspen forests and structural complexity changed more over time in pine stands. Although individual carbon pools displayed various relationships with stand age, total carbon storage increased with age, whereas carbon sequestration, inferred from changes in storage, decreased sharply with age. These results illustrate the carbon and complexity consequences of varying forest harvest rotation length to favor younger or older forests and provide insight into trade-offs between these potentially conflicting management objectives. C1 [Bradford, John B.; Kastendick, Douglas N.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Bradford, JB (reprint author), US Forest Serv, USDA, No Res Stn, 1831 Hwy,169 East, Grand Rapids, MN 55744 USA. EM jbbradford@fs.fed.us RI Bradford, John/E-5545-2011 FU US Forest Service Center for Research on Ecosystem Change; NASA Carbon Cycle Science research [CARBON/04-0225-0191, CARBON/04-0120-0011] FX The authors thank Anthony D'Amato, Shawn Fraver, and Brian Palik for insightful comments on an early version of this manuscript. Josh Kragthorpe, Samantha Mann, and Kirby Budrow provided valuable help with field data collection. This research was supported by funding from the US Forest Service Center for Research on Ecosystem Change and NASA Carbon Cycle Science research grants CARBON/04-0225-0191 and CARBON/04-0120-0011. NR 45 TC 34 Z9 39 U1 4 U2 35 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD MAR PY 2010 VL 40 IS 3 BP 401 EP 409 DI 10.1139/X10-002 PG 9 WC Forestry SC Forestry GA 580XR UT WOS:000276484600001 ER PT J AU Harrington, TB Schoenholtz, SH AF Harrington, Timothy B. Schoenholtz, Stephen H. TI Effects of logging debris treatments on five-year development of competing vegetation and planted Douglas-fir SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID PINUS-RADIATA PLANTATION; ORGANIC-MATTER REMOVAL; SOIL COMPACTION; WESTERN WASHINGTON; RESIDUE MANAGEMENT; 10-YEAR RESPONSES; HARVEST RESIDUE; BIOMASS REMOVAL; TREE SEEDLINGS; WHOLE-TREE AB Although considerable research has focused on the influences of logging debris treatments on soil and forest regeneration responses, few studies have identified whether debris effects are mediated by associated changes in competing vegetation abundance. At sites near Matlock, Washington, and Molalla, Oregon, studies were initiated after timber harvest to quantify the effects of three logging debris treatments (dispersed, piled, or removed) on the development of competing vegetation and planted Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. menziesii). Each debris treatment was replicated with initial and annual vegetation control treatments, resulting in high and low vegetation abundances, respectively. This experimental design enabled debris effects on regeneration to be separated into effects mediated by vegetation abundance and those independent of vegetation abundance. Two to three years after treatment, covers of Scotch broom (Cytisus scoparius (L.) Link) at Matlock and trailing blackberry (Rubus ursinus Cham. & Schltdl.) at Molalla were over 20% greater where debris was piled than where it was dispersed. Debris effects on vegetation abundance were associated with 30% reductions in the survival of Douglas-fir at Matlock (r(2) = 0.62) and the stem diameter at Molalla (r(2) = 0.39). Douglas-fir survival and growth did not differ among debris treatments when effects were evaluated independent of vegetation abundance (i.e., with annual vegetation control), suggesting negligible short-term effects of debris manipulation on soil productivity. C1 [Harrington, Timothy B.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia Forestry Sci Lab, Olympia, WA 98512 USA. [Schoenholtz, Stephen H.] Virginia Tech, Coll Nat Resources, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. RP Harrington, TB (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Olympia Forestry Sci Lab, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM tharrington@fs.fed.us FU USDA Forest Service Pacific Northwest Research Station; Green Diamond Resource Company; Port Blakely Tree Farms LLC; Oregon State University; Virginia Tech; Green Crow Company FX This is a product of the Sustainable Forestry component of Agenda 2020, a joint effort of the USDA Forest Service Research and Development and the American Forest and Paper Association. Funds were provided by the USDA Forest Service Pacific Northwest Research Station, Green Diamond Resource Company, Port Blakely Tree Farms LLC, Oregon State University, Virginia Tech, and Green Crow Company. The authors are especially grateful to Randall Greggs and Jeff Madsen for providing critical financial and logistical support that made this study possible. Thanks also to Connie Harrington, Tom Terry, and Ron Heninger for reviewing the study plan and to staff of the Olympia Forestry Sciences Laboratory for assisting with field and laboratory measurements. NR 49 TC 12 Z9 12 U1 1 U2 14 PU NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS PI OTTAWA PA BUILDING M 55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD MAR PY 2010 VL 40 IS 3 BP 500 EP 510 DI 10.1139/X10-001 PG 11 WC Forestry SC Forestry GA 580XR UT WOS:000276484600009 ER PT J AU Mitchell, RJ Hiers, JK O'Brien, JJ Jack, SB Engstrom, RT AF Mitchell, R. J. Hiers, J. K. O'Brien, J. J. Jack, S. B. Engstrom, R. T. TI Silviculture that sustains: the nexus between silviculture, frequent prescribed fire, and conservation of biodiversity in longleaf pine forests of the southeastern United States (vol 36, pg 2724, 2006) SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Correction C1 [Mitchell, R. J.; Hiers, J. K.; Jack, S. B.] Joseph W Jones Ecol Res Ctr, Newton, GA 39870 USA. [O'Brien, J. J.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. RP Mitchell, RJ (reprint author), Joseph W Jones Ecol Res Ctr, Route 2,Box 2324, Newton, GA 39870 USA. EM Robert.Mitchell@jonesctr.org NR 1 TC 0 Z9 0 U1 1 U2 13 PU NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS PI OTTAWA PA BUILDING M 55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD MAR PY 2010 VL 40 IS 3 BP 596 EP 596 DI 10.1139/X10-017 PG 1 WC Forestry SC Forestry GA 580XR UT WOS:000276484600018 ER PT J AU Copeland, JP McKelvey, KS Aubry, KB Landa, A Persson, J Inman, RM Krebs, J Lofroth, E Golden, H Squires, JR Magoun, A Schwartz, MK Wilmot, J Copeland, CL Yates, RE Kojola, I May, R AF Copeland, J. P. McKelvey, K. S. Aubry, K. B. Landa, A. Persson, J. Inman, R. M. Krebs, J. Lofroth, E. Golden, H. Squires, J. R. Magoun, A. Schwartz, M. K. Wilmot, J. Copeland, C. L. Yates, R. E. Kojola, I. May, R. TI The bioclimatic envelope of the wolverine (Gulo gulo): do climatic constraints limit its geographic distribution? SO CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE LA English DT Article ID HABITAT USE; HOME-RANGE; POPULATION-STRUCTURE; BRITISH-COLUMBIA; METABOLIC-RATE; NORTH-AMERICA; UNITED-STATES; GENE FLOW; MODELS; CANADA AB We propose a fundamental geographic distribution for the wolverine (Golo gulo (L., 1758)) based on the hypothesis that the occurrence of wolverines is constrained by their obligate association with persistent spring snow cover for successful reproductive denning and by an upper limit of thermoneutrality. To investigate this hypothesis, we developed a composite of MODIS classified satellite images representing persistent snow cover from 24 April to 15 May. which encompasses the end of the wolverine's reproductive denning period. To investigate the wolverine's spatial relationship with average maximum August temperatures, we used interpolated temperature maps. We then compared and correlated these climatic factors with spatially referenced data on wolverine den sites and telemetry locations from North America and Fennoscandia, and our contemporary understanding of the wolverine's circumboreal ranee. All 562 reproductive dens from Fennoscandia and North America occurred at sites with persistent spring snow cover. Ninety-five percent of summer and 86% of winter telemetry locations were concordant with spring snow coverage. Average maximum August temperature was a less effective predictor of wolverine presence. although wolverines preferred summer temperatures lower than those available. Reductions in spring snow cover associated with climatic warming will likely reduce the extent of wolverine habitat, with an associated loss of connectivity. C1 [Copeland, J. P.; McKelvey, K. S.; Squires, J. R.; Schwartz, M. K.; Yates, R. E.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Aubry, K. B.] US Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. [Landa, A.; May, R.] Norwegian Inst Nat Res, NO-7485 Trondheim, Norway. [Persson, J.] Swedish Univ Agr Sci, Dept Anim Ecol, SE-90183 Umea, Sweden. [Inman, R. M.] Wildlife Conservat Soc, Bozeman, MT 59715 USA. [Krebs, J.] Columbia Basin Fish & Wildlife Compensat Program, Nelson, BC V1L 4K3, Canada. [Lofroth, E.] Minist Environm, Victoria, BC V8W 9M1, Canada. [Golden, H.] Alaska Dept Fish & Game, Anchorage, AK 99518 USA. [Magoun, A.] Wildlife Res & Management, Fairbanks, AK 99709 USA. [Wilmot, J.] No Rockies Conservat Cooperat, Jackson, WY 83001 USA. [Copeland, C. L.] Wolverine Fdn, Kuna, ID 83634 USA. [Kojola, I.] Finnish Game & Fisheries Res Inst, FI-90570 Oulu, Finland. RP Copeland, JP (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. EM jpcopeland@fs.fed.us RI Schwartz, Michael/C-3184-2014; OI Schwartz, Michael/0000-0003-3521-3367; Persson, Jens/0000-0003-1405-7561 NR 65 TC 39 Z9 40 U1 5 U2 76 PU NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS PI OTTAWA PA BUILDING M 55, OTTAWA, ON K1A 0R6, CANADA SN 0008-4301 J9 CAN J ZOOL JI Can. J. Zool.-Rev. Can. Zool. PD MAR PY 2010 VL 88 IS 3 BP 233 EP 246 DI 10.1139/Z09-136 PG 14 WC Zoology SC Zoology GA 580XU UT WOS:000276484900001 ER PT J AU Lu, JM Zhang, KQ Nam, S Anderson, RA Jove, R Wen, W AF Lu, Jianming Zhang, Keqiang Nam, Sangkil Anderson, Richard A. Jove, Richard Wen, Wei TI Novel angiogenesis inhibitory activity in cinnamon extract blocks VEGFR2 kinase and downstream signaling SO CARCINOGENESIS LA English DT Article ID ENDOTHELIAL GROWTH-FACTOR; GRAPE SEED EXTRACT; TUMOR ANGIOGENESIS; DIETARY ANTHOCYANIDIN; BIOLOGICAL-ACTIVITY; 3T3-L1 ADIPOCYTES; INSULIN-RECEPTOR; IN-VITRO; CANCER; POLYPHENOLS AB As a critical factor in the induction of angiogenesis, vascular endothelial growth factor (VEGF) has become an attractive target for anti-angiogenesis treatment. However, the side effects associated with most anti-VEGF agents limit their chronic use. Identification of naturally occurring VEGF inhibitors derived from diet is a potential alternative approach, with the advantage of known safety. To isolate natural inhibitors of VEGF, we established an in vitro tyrosine kinase assay to screen for diet-based agents that suppress VEGFR2 kinase activity. We found that a water-based extract from cinnamon (cinnamon extract, CE), one of the oldest and most popular spices, was a potent inhibitor of VEGFR2 kinase activity, directly inhibiting kinase activity of purified VEGFR2 as well as mitogen-activated protein kinase- and Stat3-mediated signaling pathway in endothelial cells. As a result, CE inhibited VEGF-induced endothelial cell proliferation, migration and tube formation in vitro, sprout formation from aortic ring ex vivo and tumor-induced blood vessel formation in vivo. Depletion of polyphenol from CE with polyvinylpyrrolidone abolished its anti-angiogenesis activity. While cinnamaldehyde, a component responsible for CE aroma, had little effect on VEGFR2 kinase activity, high-performance liquid chromatography-purified components of CE, procyanidin type A trimer (molecular weight, 864) and a tetramer (molecular weight, 1152) were found to inhibit kinase activity of purified VEGFR2 and VEGFR2 signaling, implicating procyanidin oligomers as active components in CE that inhibit angiogenesis. Our data revealed a novel activity in cinnamon and identified a natural VEGF inhibitor that could potentially be useful in cancer prevention and/or treatment. C1 [Lu, Jianming; Zhang, Keqiang; Nam, Sangkil; Jove, Richard; Wen, Wei] City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Med, Duarte, CA 91010 USA. [Anderson, Richard A.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Wen, W (reprint author), City Hope Natl Med Ctr, Beckman Res Inst, Dept Mol Med, 1500 E Duarte Rd, Duarte, CA 91010 USA. EM wwen@coh.org FU Stop Cancer Foundation; Concern Foundation; Markel Friedman Fund FX Stop Cancer Foundation; Concern Foundation; Markel Friedman Fund. NR 50 TC 39 Z9 39 U1 1 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD MAR PY 2010 VL 31 IS 3 BP 481 EP 488 DI 10.1093/carcin/bgp292 PG 8 WC Oncology SC Oncology GA 564VE UT WOS:000275245200022 ER PT J AU Narayanaraj, G Bolstad, PV Elliott, KJ Vose, JM AF Narayanaraj, G. Bolstad, P. V. Elliott, K. J. Vose, J. M. TI Terrain and Landform Influence on Tsuga canadensis (L.) Carriere (Eastern Hemlock) Distribution in the Southern Appalachian Mountains SO CASTANEA LA English DT Article ID WOOLLY ADELGID INFESTATION; LONG-TERM; NEW-ENGLAND; SPECIES DISTRIBUTION; VEGETATION DYNAMICS; COMMUNITY STRUCTURE; FOREST RESPONSE; SOIL-MOISTURE; NATIONAL-PARK; TREE ANALYSIS AB We examined the relationships between hemlock distribution and abundance and terrain attributes for the Coweeta Basin in the southern Appalachian Mountains. Field measurements were combined with GIS mapping methods to develop predictive models of abundance and distribution of Tsuga canadensis (L.) Corriere (eastern hemlock) and evaluate the co-occurrence of Rhododendron maximum L. (rosebay) and Kalmia latifolia L. (mountain laurel). Terrain variables were derived from USGS DEM 30-meter digital maps. Elevation, slope, aspect, terrain shape index, landform, and distance from stream were calculated from field measurements and the digital data. Terrain attributes such as elevation (r(2) = 0.97, p < 0.0001), distance to stream (r(2) = 0.94, p < 0.0001), and terrain shape index (r(2) = 0.61, p = 0.0015) were good predictors of T. canadensis abundance. Terrain shape index explained 56% of the variation in R. maximum percent aerial cover (r(2) = 0.56, p = 0.005). In the Coweeta Basin, T. canadensis was distributed as few, large trees mostly concentrated in near-stream locations, and it was closely associated with R. maximum. Tsuga canadensis mortality due to Adelges tsugae Annand (hemlock wooly adelgid) will result in a minor decrease in basin-wide basal area, but will substantially reduce near-stream basal area, and will also remove the largest trees in near-stream environments. In similar landscapes across the southern Appalachians, where T. canadensis co-occurs with R. maximum, riparian shading will likely remain unchanged. C1 [Elliott, K. J.; Vose, J. M.] US Forest Serv, USDA, So Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. [Narayanaraj, G.; Bolstad, P. V.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. RP Elliott, KJ (reprint author), US Forest Serv, USDA, So Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. EM kelliott@fs.fed.us FU (University of Georgia) of the National Science Foundation [DEB 0218001]; USDA Forest Service FX We would like to thank the Coweeta Hydrologic Lab, Southern Research Station, United States Department of Agriculture, Forest Service, and the Long Term Ecological Research Program. (University of Georgia) of the National Science Foundation DEB 0218001. This research work was supported with funding from the USDA Forest Service. We would also like to thank the staff members at the Department of Forest Resources and the MGIS program at the University of Minnesota. NR 82 TC 7 Z9 8 U1 4 U2 25 PU SOUTHERN APPALACHIAN BOTANICAL SOC, NEWBERRY COLL PI NEWBERRY PA DEPT BIOLOGY, C/O CHARLES N HORN, SECRETARY-TREASURER, 2100 COLLEGE ST, NEWBERRY, SC 29108 USA SN 0008-7475 J9 CASTANEA JI Castanea PD MAR PY 2010 VL 75 IS 1 BP 1 EP 18 PG 18 WC Plant Sciences SC Plant Sciences GA 598TS UT WOS:000277862100001 ER PT J AU Yadav, MP Cooke, P Johnston, DB Hicks, KB AF Yadav, Madhav P. Cooke, Peter Johnston, David B. Hicks, Kevin B. TI Importance of Protein-Rich Components in Emulsifying Properties of Corn Fiber Gum SO CEREAL CHEMISTRY LA English DT Article ID ALKALINE PEROXIDE DELIGNIFICATION; AGRICULTURAL RESIDUES; EXTRACTION; HYDROCOLLOIDS; AGENTS; OIL AB Purified corn fiber gum (CFG-F) isolated from fine (kernel endosperm-derived) corn fiber that contained,approximate to 2% residual protein was extracted with 70% aqueous ethanol. The aqueous ethanol extract (AEE), which contained 19.5% of the total CFG, contained a high percentage of the proteinaceous material present in the original gum sample. The ACE gum contained 6.81% protein by weight. The residue (R), which constituted 66% of the total CFG-F, contained only 0.55% of protein. The emulsifying properties of R and AEE in a model oil-in-water emulsification system were studied by measuring turbidity after I, 2, and 3 weeks, particle size after 4 weeks, and by confocal laser scanning microscopy after three months of storage at room temperature. These gums were compared with the standard well-known emulsifiers native acacia gum (NAG) and modified acacia gum (MAG). The results indicate that although AEE contains protein-rich components, it is not as good an emulsifier as the residue which contains only 0.55% of protein. However, emulsions prepared with the whole (unfractionated) CFG-F under similar conditions were more stable showing higher turbidity and smaller particles size than those prepared with either R or ACE. C1 [Yadav, Madhav P.; Cooke, Peter; Johnston, David B.; Hicks, Kevin B.] ARS, Crops Convers Sci & Engn Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Yadav, MP (reprint author), ARS, Crops Convers Sci & Engn Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM madhav.yadav@ars.usda.gov NR 23 TC 18 Z9 18 U1 1 U2 8 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2010 VL 87 IS 2 BP 89 EP 94 DI 10.1094/CCHEM-87-2-0089 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 663VP UT WOS:000282918600001 ER PT J AU Arora, A Dien, BS Belyea, RL Wang, P Singh, V Tumbleson, ME Rausch, KD AF Arora, Amit Dien, Bruce S. Belyea, Ronald L. Wang, Ping Singh, Vijay Tumbleson, M. E. Rausch, Kent D. TI Laboratory Yields and Process Stream Compositions from E-Mill and Dry-Grind Corn Processes Using a Granular Starch Hydrolyzing Enzyme SO CEREAL CHEMISTRY LA English DT Article ID FERMENTATION AB In dry-grind corn processing, the whole kernel is fermented to produce ethanol and distillers dried grains with solubles (DDGS); the E-Mill process was developed to generate coproducts in addition to DDGS. Compositions of thin stillage and wet grains obtained from the E-Mill process will be different from the dry-grind process. Knowledge of thin stillage compositions will provide information to improve coproducts from both processes. Laboratory dry-grind and E-Mill processes that used granular starch hydrolyzing enzymes (GSHE) were compared and process yields determined. Two methods, centrifugation and screening, were used to produce thin stillage and wet grains from the laboratory processes. Compositions of process streams were determined. In the dry-grind process using GSHE, solids contents of beer, whole stillage, and wet grains were higher compared to the same fractions from the E-Mill process using GSHE. Solids contents of mash for both processes were similar. Total solids, soluble solids, and ash contents of thin stillage were similar for the two processes. Fat content of thin stillage from E-Mill was lower than that from the dry-grind process; protein content of E-Mill thin stillage was higher than that from dry-grind thin stillage. Removal of germ and fiber before fermentation changed composition of thin stillage from the E-Mill process. The screening method produced higher thin stillage and lower wet grains yields than using a centrifugation method. The screening method was less time consuming but resulted in limited wet grains material for additional analyses or processing. The centrifugation method of thin stillage separation removed more solids from thin stillage than the screening method. C1 [Arora, Amit; Wang, Ping; Singh, Vijay; Tumbleson, M. E.; Rausch, Kent D.] Univ Illinois, Urbana, IL 61801 USA. [Dien, Bruce S.] ARS, Fermentat Biochem Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Belyea, Ronald L.] Univ Missouri, Columbia, MO 65211 USA. RP Rausch, KD (reprint author), Univ Illinois, Urbana, IL 61801 USA. EM krausch@illinois.edu OI Dien, Bruce/0000-0003-3863-6664 NR 13 TC 2 Z9 2 U1 0 U2 6 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2010 VL 87 IS 2 BP 100 EP 103 DI 10.1094/CCHEM-87-2-0100 PG 4 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 663VP UT WOS:000282918600003 ER PT J AU Tsilo, TJ Ohm, JB Hareland, GA Anderson, JA AF Tsilo, Toi J. Ohm, Jae-Bom Hareland, Gary A. Anderson, James A. TI Association of Size-Exclusion HPLC of Endosperm Proteins with Dough Mixing and Breadmaking Characteristics in a Recombinant Inbred Population of Hard Red Spring Wheat SO CEREAL CHEMISTRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; MOLECULAR-WEIGHT DISTRIBUTION; FLOUR PROTEINS; WINTER-WHEAT; QUALITY CHARACTERISTICS; BAKING QUALITY; BREAD WHEATS; GLUTENIN; ENVIRONMENT; PREDICTION AB Variation of polymeric proteins affects wheat end-use quality. This research investigated associations of polymeric proteins with dough mixing strength and breadmaking characteristics in a near-homogenous population of 139 recombinant inbred lines (RILs) derived from a cross between two hard red spring wheat breeding lines. Flours from the RILs grown at three locations were analyzed for molecular weight (MW) distribution of SDS-extractable and unextractable proteins using size-exclusion HPLC protocol. Correlations were calculated between mixing and breadmaking properties and HPLC absorbance data obtained a 0.01-min retention time interval to identify protein fractions that had a significant effect on the quality traits. Very high MW polymeric proteins in the unextractable fraction had more distinct and positive associations with dough mixing strength and bread loaf volume than did other polymeric protein fractions, whereas extractable polymeric had negative influence. Consequently, the ratio of unextractable very high MW polymeric proteins to extractable polymeric proteins had greater correlations with dough mixing parameters than other HPLC absorbance area data. Covariate-effect biplots also visually validated positive effects of unextractable very high MW polymeric proteins and negative effects of extractable polymeric proteins on mixing properties and loaf volume across three growing locations. C1 [Ohm, Jae-Bom; Hareland, Gary A.] USDA ARS RRVARC NCSL, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, NDSU Dept 7640, Fargo, ND 58102 USA. [Tsilo, Toi J.; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. RP Ohm, JB (reprint author), USDA ARS RRVARC NCSL, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, NDSU Dept 7640, POB 6050, Fargo, ND 58102 USA. EM jae.ohm@ars.usda.gov RI Tsilo, Toi/G-5088-2011 FU Minnesota Annual Conference of the United Methodist Church; National Research Foundation of South Africa; Department of Science and Technology of South Africa; Agricultural Research Council of South Africa; United State Department of Agriculture-Agricultural Research Service FX We gratefully acknowledge financial support from the Minnesota Annual Conference of the United Methodist Church, the Compton International fellowship, National Research Foundation of South Africa, the Department of Science and Technology of South Africa, Agricultural Research Council of South Africa, and the United State Department of Agriculture-Agricultural Research Service. We would like to thank the University of Minnesota wheat breeding scientists, Gary Linkert and Catherine Springer, and the USDA/ARS personnel, Dale Hanson, Angela Ostenson, Dehdra Puhr, and Jadene Wear, for their technical assistance. NR 34 TC 22 Z9 22 U1 1 U2 7 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2010 VL 87 IS 2 BP 104 EP 111 DI 10.1094/CCHEM-87-2-0104 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 663VP UT WOS:000282918600004 ER PT J AU Lalzar, I Simon, T Meer, RKV Hefetz, A AF Lalzar, I. Simon, T. Meer, R. K. Vander Hefetz, Abraham TI Alteration of cuticular hydrocarbon composition affects heterospecific nestmate recognition in the carpenter ant Camponotus fellah SO CHEMOECOLOGY LA English DT Article DE Cuticular hydrocarbons; Nestmate recognition; Camponotus fellah ID DIRECT BEHAVIORAL EVIDENCE; CUES; ODOR; DISCRIMINATION; AGGRESSION; SIGNATURE; WORKERS; HUMILE AB Nestmate recognition is a ubiquitous phenomenon in social insects as a means to prevent entry of undesired individuals aiming at exploiting the rich nest resources. The recognition cues in ants were shown in a few cases to be cuticular hydrocarbons, although there are a quite number of correlated associations. In the present study we modified the cuticular profiles of workers Camponotus fellah hydrocarbons with cuticular washes from a closely related, yet undescribed species, Camponotus sp. Although these sympatric species are morphologically indistinguishable, cuticular washes of C. sp. contain 9,13-dimethylpentacosane and 11,15-dimethylheptacosane that are either absent or occur as traces in C. fellah. In addition, C. sp. contains significantly greater amounts of 3-methylpentacosane than C. fellah workers. The cuticle modification was done solventless in a manner that minimized disruption to the cuticular structure of the ant being modified. Judging from the 3 focal compounds, such treatment added between 20 and 30% of the original amounts present in C. sp. to the treated C. fellah workers. This addition changed consistently the cuticular hydrocarbon profile of the treated ant. Dyadic assays between C. fellah and their nestmates treated with C. sp. cuticular rinses revealed a significantly higher level of aggression compared to non-treated nestmates. There was no aggression between nestmates of C. sp. These results demonstrate that in heterospecific interactions between the two Camponotus species there is a correlation between cuticular hydrocarbons and a nestmate recognition response, albeit not as high as the response of C. fellah to of C. sp. workers. This is consistent with the hypothesis that cuticular hydrocarbons may play a role in nestmate recognition. C1 [Lalzar, I.; Simon, T.; Hefetz, Abraham] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Ramat Aviv, Israel. [Meer, R. K. Vander] ARS, CMAVE, USDA, Gainesville, FL 32608 USA. RP Hefetz, A (reprint author), Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Ramat Aviv, Israel. EM hefetz@post.tau.ac.il RI Hefetz, Abraham/I-3853-2015 FU United States-Israel BiNational Science Foundation [203367] FX This research was supported by The United States-Israel BiNational Science Foundation (Grant n 203367 to Abraham Hefetz & Robert K. Vander Meer). We thank N. Paz for editorial assistance. The experiments comply with the current laws of Israel. NR 28 TC 7 Z9 7 U1 0 U2 15 PU BIRKHAUSER VERLAG AG PI BASEL PA VIADUKSTRASSE 40-44, PO BOX 133, CH-4010 BASEL, SWITZERLAND SN 0937-7409 J9 CHEMOECOLOGY JI Chemoecology PD MAR PY 2010 VL 20 IS 1 BP 19 EP 24 DI 10.1007/s00049-009-0030-x PG 6 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 567CN UT WOS:000275420000003 ER PT J AU Wang, Z Gehlhar, M Yao, SL AF Wang, Zhi Gehlhar, Mark Yao, Shunli TI A globally consistent framework for reliability-based trade statistics reconciliation in the presence of an entrepot SO CHINA ECONOMIC REVIEW LA English DT Article DE Trade statistics reconciliation; Entrepot trade; Data reliability; Global consistency ID BALANCES; ACCOUNTS AB This paper develops a mathematical programming model to reconcile trade statistics subject to a set of global consistency conditions in the presence of an entrepot. Initial data reliability serves a key function for governing the magnitude of adjustment. Through a two-stage optimization procedure, the adjusted trade statistics are achieved as solutions to a system of simultaneous equations that minimize a quadratic penalty function. As an empirical illustration. the model is applied to reconcile the 2004 trade statistics reported by China, Hong Kong and their major trading partners, initialized with detailed estimates of bilateral trade flows, re-export markups, cif/fob ratios and data reliability indexes. Published by Elsevier Inc. C1 [Gehlhar, Mark] USDA, Washington, DC USA. [Yao, Shunli] Univ Int Business & Econ, Beijing, Peoples R China. EM Zhi.Wang@usitc.gov; mgehlhar@ers.usda.gov; slyao@uibe.edu.cn NR 31 TC 2 Z9 2 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1043-951X J9 CHINA ECON REV JI China Econ. Rev. PD MAR PY 2010 VL 21 IS 1 BP 161 EP 189 DI 10.1016/j.chieco.2009.12.003 PG 29 WC Economics SC Business & Economics GA 572AY UT WOS:000275799800013 ER PT J AU Hesler, LS Kieckhefer, RW Mesman, A Catangui, MA AF Hesler, Louis S. Kieckhefer, Robert W. Mesman, Amy Catangui, Michael A. TI COCCINELLIDAE (COLEOPTERA) OF SOUTH DAKOTA: NEW STATE RECORD FOR ANATIS LECONTEI CASEY AND ERRATUM TO DELETE HYPERASPIS FIMBRIOLATA MELSHEIMER SO COLEOPTERISTS BULLETIN LA English DT Editorial Material C1 [Hesler, Louis S.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Mesman, Amy] USDA, APHIS, PPQ, Pierre, SD 57501 USA. [Catangui, Michael A.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. RP Hesler, LS (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov NR 4 TC 0 Z9 0 U1 2 U2 2 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X J9 COLEOPTS BULL JI Coleopt. Bull. PD MAR PY 2010 VL 64 IS 1 BP 84 EP 84 DI 10.1649/0010-065X-64.1.84 PG 1 WC Entomology SC Entomology GA 587UM UT WOS:000277019200015 ER PT J AU Jensen, AE Halling, SM AF Jensen, Allen E. Halling, Shirley M. TI Effect of polymyxin B and environmental conditions on isolation of Brucella species and the vaccine strain RB51 SO COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES LA English DT Article DE BvrR; CO(2)-independent; MIC; Brucellosis; Selective media; Marine brucellae; Etest; Colistin ID BACTERICIDAL CATIONIC PEPTIDES; MEMBRANE-PROTEINS; OUTER-MEMBRANE; ABORTUS; ANTIBIOTICS; MELITENSIS; LIPOPOLYSACCHARIDE; SUSCEPTIBILITY; RESISTANCE; INFECTION AB Brucella are resistant to polymyxin B (PB), but their relative susceptibility to PB and its derivative, colistin (COL) has not been rigorously or systematically studied. Comparative susceptibility of Brucella reference strains, vaccine strain RB51, and Brucella isolates from marine mammals to these two cationic peptides were determined by Etest. Vast differences among Brucella species were found in susceptibility to both PB and COL. Brucella demonstrated similar pattern of relative susceptibility to PB as that of COL, but they were less susceptible to COL. Both B. melitensis and B. suis were the least susceptible to polymyxins and rough strains were more susceptible to both PB and COL than the smooth except for the BvrR mutant. Strains were generally less susceptible to PB when cultured in CO(2) rather than ambient air; some became more susceptible in acidified medium. Results show that environment cultural conditions must be considered when selecting for CO(2)-independent strains of Brucella especially the vaccine strain RB51 on selective media containing PB. Our observations extend basic knowledge of the differential resistance of Brucella to polymyxins. Published by Elsevier Ltd. C1 [Jensen, Allen E.; Halling, Shirley M.] ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Jensen, AE (reprint author), ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, 2300 Dayton Ave, Ames, IA 50010 USA. EM allen.jensen@ars.usda.gov NR 37 TC 3 Z9 4 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0147-9571 J9 COMP IMMUNOL MICROB JI Comp. Immunol. Microbiol. Infect. Dis. PD MAR PY 2010 VL 33 IS 2 BP 121 EP 131 DI 10.1016/j.cimid.2008.08.005 PG 11 WC Immunology; Microbiology; Veterinary Sciences SC Immunology; Microbiology; Veterinary Sciences GA 570NC UT WOS:000275684000003 PM 18814911 ER PT J AU Balint-Kurti, PJ Yang, JY Van Esbroeck, G Jung, J Smith, ME AF Balint-Kurti, Peter J. Yang, Junyun Van Esbroeck, George Jung, Janelle Smith, Margaret E. TI Use of a Maize Advanced Intercross Line for Mapping of QTL for Northern Leaf Blight Resistance and Multiple Disease Resistance SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCI; ZEA-MAYS L; CHLOROTIC-LESION RESISTANCE; HETEROSTROPHUS RACE-O; HOST-PLANT RESPONSE; EXSEROHILUM-TURCICUM; HELMINTHOSPORIUM-TURCICUM; SETOSPHAERIA-TURCICA; SWEET CORN; LATENT PERIOD AB Northern leaf blight [NLB; caused by Exserohilum turcicum (Pass) K.J. Leonard and E.G. Suggs] is an important fungal disease of maize (Zea mays L.) in the United States and worldwide. The IBM population, an advanced intercross recombinant inbred line population derived from a cross between the lines Mo17 and B73, was evaluated in three environments (Aurora, NY, in 2006 and 2007 and Clayton, NC in 2007) for two traits related to NLB resistance, weighted mean disease (WMD) and incubation period (IP), and for days to anthesis (DTA). Two WMD quantitative trait loci (QTL) in bins 2.00/2.01 and 4.08 were detected from the overall analysis; of these, only the QTL in bin 4.08 was detected in all three environments analyzed separately. Likewise, only one IP QTL, in bin 2.02, was detected in all three environments and from the overall analysis. Several environment-specific OTL for each trait were also detected. Several DTA QTL were detected with the strongest effect detected in bin 8.05. Correlations between disease resistance traits and days to anthesis were uniformly low. The results from this study were compared to those of previous studies that used the IBM population to identify OTL for two other maize foliar diseases, southern leaf blight (causal agent Cochliobolus heterostrophus (Drechs.) Drechs. [anamorph = Bipolaris maydis (Nisikado and Miyake) Shoemaker; synonym = Helminthosporium maydis (Nisikado and Miyake)]} and gray leaf spot [causal agent Cercospora zeae-maydis (Tehon and E.Y. Daniels)]. Although we did not find QTL conferring resistance to all three diseases, significant correlations between resistances to these diseases in the IBM population were identified, implying the existence of loci (and possibly genes) affecting resistance to all three diseases. C1 [Balint-Kurti, Peter J.] USDA ARS, Plant Sci Res Unit, Washington, DC 20250 USA. [Balint-Kurti, Peter J.; Van Esbroeck, George] N Carolina State Univ, Dep Plant Pathol, Raleigh, NC 27695 USA. [Yang, Junyun; Jung, Janelle; Smith, Margaret E.] Cornell Univ, Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Balint-Kurti, PJ (reprint author), USDA ARS, Plant Sci Res Unit, Washington, DC 20250 USA. EM peter_balintkurti@ncsu.edu OI Balint-Kurti, Peter/0000-0002-3916-194X FU USDA-ARS; Corn Growers Association of North Carolina, Inc; USDA [NYC149-466]; College of Agriculture and Life Sciences at Cornell University FX The authors wish to thank Donna Stephens, Ann Stapleton, Steve Szalnia, Jim Holland, Jacob Burgdorf, Sherrie Norman, Laraine Ericson, Keith Payne, Chia-Lin Chung, and Randy Wisser for assistance with various aspects of this work. This work was funded by the USDA-ARS, by a grant from the Corn Growers Association of North Carolina, Inc., and by support from USDA Hatch project NYC149-466 and from the College of Agriculture and Life Sciences at Cornell University. This paper is dedicated to the memory of Donna Stephens. NR 53 TC 15 Z9 15 U1 3 U2 25 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 458 EP 466 DI 10.2135/cropsci2009.02.0066 PG 9 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500004 ER PT J AU Warburton, ML Setimela, P Franco, J Cordova, H Pixley, K Banziger, M Dreisigacker, S Bedoya, C MacRobert, J AF Warburton, Marilyn L. Setimela, Peter Franco, Jorge Cordova, Hugo Pixley, Kevin Baenziger, Marianne Dreisigacker, Susanne Bedoya, Claudia MacRobert, John TI Toward a Cost-Effective Fingerprinting Methodology to Distinguish Maize Open-Pollinated Varieties SO CROP SCIENCE LA English DT Article ID INBRED LINES; GENETIC DIVERSITY; POPULATIONS AB In Africa, many smallholder farmers grow open-pollinated maize (Zea mays L.) varieties (OPVs), which allow seed recycling and outyield traditional unimproved landraces. Seeds of productive OPVs are provided to farmers, often by nongovernmental organizations (NGOs) that help farmers access improved seeds, particularly following disasters in which original seed is lost. However, NGOs often rely on local seed suppliers to provide seed, and in some years the seeds provided to the farmers are suspected not to be of the promised variety. Here we present methodology to prove within a high level of confidence if two samples of seeds are the same genetic population or not, despite the difficulties involved in fingerprinting heterologous populations. In addition to heterogeneity within populations, difficulties can include sampling errors, differences in the fields or years in which the seeds were multiplied, and seed mixing. Despite these confounding sources of variation, we show the possibility to conclusively differentiate each of the populations used in this work. This methodology will allow breeders, seed companies, government agencies, and NGOs to ensure the purity and identity of high-yielding, locally adapted OPVs reach farmers so they can generate the highest yields possible in their fields. C1 [Setimela, Peter; MacRobert, John] CIMMYT, Maize Program, Harare, Zimbabwe. [Warburton, Marilyn L.] Mississippi State Univ, USDA ARS CHPRRU, Mississippi State, MS 39762 USA. [Franco, Jorge] Univ Republica, Fac Agron, Montevideo, Uruguay. [Cordova, Hugo; Pixley, Kevin; Dreisigacker, Susanne; Bedoya, Claudia] CIMMYT, Mexico City 06600, DF, Mexico. RP Setimela, P (reprint author), CIMMYT, Maize Program, POB MP 163, Harare, Zimbabwe. EM p.setimela@cgiar.org NR 22 TC 11 Z9 12 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 467 EP 477 DI 10.2135/cropsci2009.02.0089 PG 11 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500005 ER PT J AU Lewis, MF Lorenzana, RE Jung, HJG Bernardo, R AF Lewis, Magan Friskop Lorenzana, Robenzon E. Jung, Hans-Joachim G. Bernardo, Rex TI Potential for Simultaneous Improvement of Corn Grain Yield and Stover Quality for Cellulosic Ethanol SO CROP SCIENCE LA English DT Article ID BREEDING PROGRAM; MAIZE; SELECTION; POPULATION; SACCHARIFICATION; PRETREATMENT; REMOVAL; BIOMASS; TRAITS; LIGNIN AB Corn (Zea mays L.) stover, the residue left after harvest, is an abundant biomass source for producing cellulosic ethanol in the United States. Corn has been bred for increased grain yield but not for stover quality for cellulosic ethanol production. Our objective in this study was to assess the feasibility of breeding corn for grain yield and agronomic traits as well as stover quality traits for cellulosic ethanol production. Testcrosses of 223 B73 x Mo17 recombinant inbreds were evaluated at four Minnesota locations in 2007. Three stover quality traits were measured: concentration of cell wall glucose in dry stover ("Glucose"); cell wall glucose released from the stover by thermochemical pretreatment and enzymatic saccharification ("Glucose Release"); and concentration of lignin on a cell wall basis ("Lignin"). Genetic variances were significant for grain yield, moisture stalk and root lodging, plant height, and all three stover quality traits. Heritabilities of the stover quality traits were 0.57 for Glucose, 0.63 for Glucose Release, and 0.68 for Lignin. Phenotypic and genetic correlations were favorable or neutral among grain yield, agronomic traits, Glucose, Glucose Release, and Lignin. Lines selected with a multiple-trait index for grain yield, agronomic traits, and stover quality traits had improved means for each trait in the index. Current corn breeding programs should be able to incorporate stover quality for cellulosic ethanol as a breeding objective, without having to use unadapted or exotic germplasm and without adversely affecting genetic gains for grain yield and agronomic traits. C1 [Lewis, Magan Friskop; Lorenzana, Robenzon E.; Jung, Hans-Joachim G.; Bernardo, Rex] Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. [Jung, Hans-Joachim G.] USDA ARS, St Paul, MN 55108 USA. RP Bernardo, R (reprint author), Univ Minnesota, Dep Agron & Plant Genet, 411 Borlaug Hall,1991 Upper Buford Cir, St Paul, MN 55108 USA. EM bernardo@umn.edu FU USDA NRI FX We thank Tom Hoverstad, Richard Jeo, Steve Quiring, and Eric Ristau for their excellent technical assistance, and Monsanto for the use of one of their proprietary inbreds as a tester. This research was funded by a USDA NRI Plant Feedstock Genomics for Bioenergy grant. NR 46 TC 28 Z9 29 U1 0 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 516 EP 523 DI 10.2135/cropsci2009.03.0148 PG 8 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500009 ER PT J AU Lorenzana, RE Lewis, MF Jung, HJG Bernardo, R AF Lorenzana, Robenzon E. Lewis, Magan Friskop Jung, Hans-Joachim G. Bernardo, Rex TI Quantitative Trait Loci and Trait Correlations for Maize Stover Cell Wall Composition and Glucose Release for Cellulosic Ethanol SO CROP SCIENCE LA English DT Article ID FALSE DISCOVERY RATE; RECOMBINANT INBRED LINES; STEM TISSUES; MOLECULAR MARKERS; COMPLEX TRAITS; CORN STOVER; LIGNIN; DEGRADABILITY; SELECTION; BIOMASS AB In cellulosic ethanol production, the efficiency of converting maize (Zea mays L.) stover into fermentable sugars partly depends on the stover cell wall structure. Breeding for improved stover quality for cellulosic ethanol may benefit from the use of molecular markers. However, limited quantitative trait loci (QTL) studies have been published for maize stover cell wall components, and no QTL study has been published for glucose release (GLCRel) from stover by a cellulosic ethanol conversion process. Our objectives were to characterize the relationships among stover cell wall components and GLCRel, and to identify QTL with major effects, if any, influencing stover cell wall composition and GLCRel. Testcrosses of 223 intermated B73 x Mo17 recombinant inbreds and the parent lines were analyzed for cell wall composition and GLCRel after acid pretreatment and enzymatic hydrolysis. As expected, glucose (GLC), xylose (XYL), and Klason lignin (KL) composed the bulk (similar to 72%) of the stover dry matter. Significant genetic variance and moderate heritability were observed for all traits. Genetic and phenotypic correlations among traits were generally in the favorable direction but also reflected the complexity of maize stover cell wall composition. We found 152 QTL, mostly with small effects, for GLCRel and cell wall components on both a dry matter and cell wall basis. Because no major QTL were found, methods that increase the frequency of favorable QTL alleles or that predict performance based on markers would be appropriate in marker-assisted breeding for maize stover quality for cellulosic ethanol. C1 [Lorenzana, Robenzon E.; Lewis, Magan Friskop; Jung, Hans-Joachim G.; Bernardo, Rex] Univ Minnesota, Dep Agron & Plant Genet, Minneapolis, MN 55455 USA. [Jung, Hans-Joachim G.] USDA ARS, St Paul, MN 55108 USA. RP Bernardo, R (reprint author), Univ Minnesota, Dep Agron & Plant Genet, Minneapolis, MN 55455 USA. EM bernardo@umn.edu NR 69 TC 28 Z9 29 U1 1 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 541 EP 555 DI 10.2135/cropsci2009.04.0182 PG 15 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500012 ER PT J AU Hamblin, MT Close, TJ Bhat, PR Chao, SM Kling, JG Abraham, KJ Blake, T Brooks, WS Cooper, B Griffey, CA Hayes, PM Hole, DJ Horsley, RD Obert, DE Smith, KP Ullrich, SE Muehlbauer, GJ Jannink, JL AF Hamblin, Martha T. Close, Timothy J. Bhat, Prasanna R. Chao, Shiaoman Kling, Jennifer G. Abraham, K. Joseph Blake, Tom Brooks, Wynse S. Cooper, Blake Griffey, Carl A. Hayes, Patrick M. Hole, David J. Horsley, Richard D. Obert, Donald E. Smith, Kevin P. Ullrich, Steven E. Muehlbauer, Gary J. Jannink, Jean-Luc TI Population Structure and Linkage Disequilibrium in US Barley Germplasm: Implications for Association Mapping SO CROP SCIENCE LA English DT Article ID WHOLE-GENOME ASSOCIATION; GENETIC DIVERSITY; FERTILE CRESCENT; DOMESTICATION; POLYMORPHISM; ADAPTATION; RATES; EAST; MAPS AB Previous studies have shown that there is considerable population structure in cultivated barley (Hordeum vulgare L.), with the strongest structure corresponding to differences in row number and growth habit. U.S. barley breeding programs include six-row and two-row types and winter and spring types in all combinations. To facilitate mapping of complex traits in breeding germplasm, 1816 barley lines from 10 U.S. breeding programs were scored with 1536 single nucleotide polymorphism (SNP) genotyping assays. The number of SNPs segregating within breeding programs varied from 854 to 1398. Model-based analysis of population structure showed the expected clustering by row type and growth habit; however, there was additional structure, some of which corresponded to the breeding programs. The model that fit the data best had seven populations: three two-row spring, two six-row spring, and two six-row winter. Average linkage disequilibrium (LD) within populations decayed over a distance of 20 to 30 cM, but some populations showed long-range LID suggestive of admixture. Genetic distance (allele-sharing) between populations varied from 0.11 (six-row spring vs. six-row spring) to 0.45 (two-row spring vs. six-row spring). Analyses of pairwise LID revealed that the phase of allelic associations was not well correlated between populations, particularly when their allele-sharing distance was >0.2. These results suggest that pooling divergent barley populations for purposes of association mapping may be inadvisable. C1 [Hamblin, Martha T.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Close, Timothy J.] Univ Calif Riverside, Dep Bot & Plant Sci, Riverside, CA 92521 USA. [Bhat, Prasanna R.] Monsanto Res Ctr, Bangalore 560092, Karnataka, India. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Kling, Jennifer G.; Hayes, Patrick M.] Oregon State Univ, Dep Crop & Soil Sci, Corvallis, OR 97331 USA. [Abraham, K. Joseph] Case Western Reserve Univ, Dep Epidemiol & Biostat, Cleveland, OH 44106 USA. [Blake, Tom] Montana State Univ, Dep Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Brooks, Wynse S.; Griffey, Carl A.] Virginia Tech, Crop & Soil Environm Sci Dep, Blacksburg, VA 24061 USA. [Cooper, Blake] Busch Agr Resources Inc, Ft Collins, CO 80524 USA. [Hole, David J.] Utah State Univ, Logan, UT 84322 USA. [Horsley, Richard D.] N Dakota State Univ, Dep Plant Sci, Fargo, ND 58108 USA. [Obert, Donald E.] Small Grains & Potato Res Facil, Aberdeen, ID 83210 USA. [Smith, Kevin P.; Muehlbauer, Gary J.] Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. [Ullrich, Steven E.] Washington State Univ, Dep Crop & Soil Sci, Pullman, WA 99164 USA. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM jeanluc.jannink@ars.usda.gov RI Hole, David/A-7173-2008; s, d/B-2182-2010 OI Hole, David/0000-0003-1325-3181; FU USIDA-CSREES-NRI [2006-55606-16722] FX Hiroyoshi Iwata and Peter Bradbury provided valulble comments during the course of this study. This research was Supported by USIDA-CSREES-NRI Grant no. 2006-55606-16722 "Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement." MTH and JLJ designed the study, and analyzed and interpreted the data. MTH wrote the paper. PRB, SC, and TJC designed, implemented, and scored the SNP genotyping assays. JGK curated Barley CAP core pedigree and Barley CAP germplasm data. KJA wrote the algorithm used to select unassociatcd markers. TB, WSB, BC, CC, PH, DH, RH, DO, KS, and SEU selected the germplasm and contributed knowledge of it; they are listed in alphabetical order. GJM is Project Director of the Barley CAP project, which generated the data, and of which this work is a part. NR 38 TC 56 Z9 60 U1 2 U2 19 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 556 EP 566 DI 10.2135/cropsci2009.04.0198 PG 11 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500013 ER PT J AU Miranda, LM Bland, DE Cambron, SE Lyerly, JH Johnson, J Buntin, GD Murphy, JP AF Miranda, L. M. Bland, D. E. Cambron, S. E. Lyerly, J. H. Johnson, J. Buntin, G. D. Murphy, J. P. TI Genetic Mapping of an Aegilops tauschii-derived Hessian Fly Resistance Gene in Common Wheat SO CROP SCIENCE LA English DT Article ID TRITICUM-TAUSCHII; BREAD WHEAT; D-GENOME; CECIDOMYIIDAE POPULATIONS; MICROSATELLITE MARKERS; UNITED-STATES; DIPTERA; H13; IDENTIFICATION; VIRULENCE AB Hessian fly [Mayetiola destructor (Say)] is a major threat to wheat (Triticum aestivum L.) production in the eastern United States. Cultivar releases containing major Hessian fly resistance genes have proven effective in minimizing losses caused by this pest. Nevertheless, the ephemeral nature of major gene resistance necessitates the identification of novel sources of resistance. Hessian fly resistance from Aegilops tauschii Coss. (2n = 2x = 14; genome DD) was introgressed into the genetic background of the soft red winter wheat cultivar Saluda during the development of the germplasm line NC09MDD14. Our genetic characterization and linkage mapping studies showed that resistance to Hessian Fly biotype L in NC09MDD14 was monogenic and the most likely order of the linked microsatellite markers was: Xcfd13- 4.2 cM-Xcfd42-1.8cM-Xgdm141-4.9cM-Xgdm36-1.5 cM- NC09MDD14 Hf gene/Xcfd132- 13.4 cM- Xcfd19. This linkage map situated the NCD-09MDD14 Hf gene on the short arm of chromosome 6D, within the same deletion bin as the named gene H13. No recombinants between H13 and the NCD-09MDD14 Hf gene were found in an allelism study that included 170 F(2) individuals from the cross between NCD09MDD14 and Molly (H13). The Hessian fly resistance gene present in the germplasm line NC09MDD14 could be an allele of H13, but unlike H13, the gene in NC09MDD14 provides resistance against biotype vrH13. C1 [Miranda, L. M.] USDA ARS, Raleigh, NC 27607 USA. [Cambron, S. E.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Lyerly, J. H.; Murphy, J. P.] N Carolina State Univ, Dep Crop Sci, Raleigh, NC 27695 USA. [Bland, D. E.; Johnson, J.] Univ Georgia, Dep Crop Sci, Griffin, GA 30223 USA. [Buntin, G. D.] Univ Georgia, Dep Entomol, Griffin, GA 30223 USA. RP Miranda, LM (reprint author), USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. EM Lilian.Miranda@ars.usda.gov NR 28 TC 1 Z9 2 U1 2 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 612 EP 616 DI 10.2135/cropsci2009.05.0278 PG 5 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500019 ER PT J AU Irish, BM Goenaga, R Zhang, DP Schnell, R Brown, JS Motamayor, JC AF Irish, Brian M. Goenaga, Ricardo Zhang, Dapeng Schnell, Raymond Brown, J. Steve Motamayor, Juan Carlos TI Microsatellite Fingerprinting of the USDA-ARS Tropical Agriculture Research Station Cacao (Theobroma cacao L.) Germplasm Collection SO CROP SCIENCE LA English DT Article ID GENETIC DIVERSITY; POPULATION-STRUCTURE; ACCESSIONS; DIFFERENTIATION; IDENTIFICATION; CONSERVATION; VARIABILITY; SIMULATION; RICHNESS; MARKERS AB Cacao (Theobroma cacao L.) is an important cash crop in many tropical countries. Cacao accessions must be propagated vegetatively to conserve genetic integrity due to its allogamous nature and its seed recalcitrance (lack of dormancy). Therefore, cacao germplasm is usually maintained as living trees in field collections and has resulted in varying rates of misidentification and duplication. Using a high throughput genotyping system with 15 microsatellite loci, all 924 trees in the USDA-ARS Mayaguez cacao collection were fingerprinted. Nineteen accessions (12.3%) were found to have intraplant errors while 14 (9.1%) synonymous sets were identified that included replicates of 49 accessions. The average number of alleles (8.8; SE = 0.56) and gene diversity (H(Obs). = 0.65; SE = 0.026) indicate a high allelic diversity in this collection. A distance-based cluster analysis and a Bayesian assignment test showed that the cacao accessions can be classified into four distinct clusters, with their geographical origins covering most of the cacao growing regions in the Americas. Assessment of the representative diversity of the collection led to the identification of several genetic gaps, including underrepresented genetic populations and particular traits of economic and agronomic value. The improved understanding of identities and structure in the USDA-ARS cacao collection will contribute to more efficient use of cacao in conservation and breeding. C1 [Irish, Brian M.; Goenaga, Ricardo] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Zhang, Dapeng] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Motamayor, Juan Carlos] USDA ARS, Mars Inc, Subtrop Hort Res Stn, Miami, FL 33158 USA. RP Irish, BM (reprint author), USDA ARS, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM Brian.Irish@ars.usda.gov NR 45 TC 27 Z9 27 U1 0 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 656 EP 667 DI 10.2135/cropsci2009.06.0299 PG 12 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500025 ER PT J AU Olson, EL Brown-Guedira, G Marshall, DS Jin, Y Mergoum, M Lowe, L Dubcovsky, J AF Olson, Eric L. Brown-Guedira, Gina Marshall, David S. Jin, Yue Mergoum, Mohamed Lowe, Lago Dubcovsky, Jorge TI Genotyping of US Wheat Germplasm for Presence of Stem Rust Resistance Genes Sr24, Sr36 and Sr1RS(Amigo) SO CROP SCIENCE LA English DT Article ID F-SP TRITICI; EASTERN AFRICA; UNITED-STATES; ADULT-PLANT; RACE TTKS; VIRULENCE; MARKERS; REGISTRATION; SPREAD AB The stem rust resistance genes Sr24, Sr26, Sr36, and Sr1RS(Amigo) confer resistance to race TTKSK (= Ug99) of Puccinia graminis f. sp. tritici Pers. (Pgt). A collection of 776 cultivars and breeding lines of wheat (Triticum aestivum L.) from all growing regions of the United States were screened with simple sequence repeat and sequence tagged site markers linked to Sr24, Sr26, Sr36, and Sr1RS(Amigo) to determine frequencies of these genes in U.S. wheat germ-plasm. Marker efficacy in predicting the presence of these genes was evaluated via comparison with assayed seedling infection type. Among the lines evaluated, the most predominant gene is Sr24, present in hard winter, hard spring, and soft winter wheat lines. Resistance in soft winter wheat is primarily due to Sr36. The 1RS.1AL rye translocation carrying Sr1RS(Amigo) is present at equal frequencies in hard winter and soft winter wheat. Utilization of marker-assisted selection for stem rust resistance genes can hasten the development of wheat cultivars resistant to TTKSK and its variants and allow for the development of resistance gene pyramids for more durable stem rust resistance. C1 [Brown-Guedira, Gina] N Carolina State Univ, USDA ARS Plant Sci Res, Dep Crop Sci, Raleigh, NC 27695 USA. [Marshall, David S.] N Carolina State Univ, USDA ARS Plant Sci Res, Dep Plant Pathol, Raleigh, NC 27695 USA. [Jin, Yue] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Mergoum, Mohamed] N Dakota State Univ, Dep Plant Sci, Fargo, ND 58105 USA. [Lowe, Lago; Dubcovsky, Jorge] Univ Calif Davis, Dep Plant Sci, Davis, CA 95616 USA. RP Brown-Guedira, G (reprint author), N Carolina State Univ, USDA ARS Plant Sci Res, Dep Crop Sci, Campus Box 7620, Raleigh, NC 27695 USA. EM Gina.Brown-Guedira@ars.usda.gov RI Mergoum, Mohamed/D-3815-2014; Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (CSREES) [2006-55606-16629] FX This project was supported in part by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (CSREES) CAP grant number 2006-55606-16629. NR 32 TC 16 Z9 16 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 668 EP 675 DI 10.2135/cropsci2009.04.0218 PG 8 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500026 ER PT J AU Venuto, BC Daniel, JA AF Venuto, B. C. Daniel, J. A. TI Biomass Feedstock Harvest from Conservation Reserve Program Land in Northwestern Oklahoma SO CROP SCIENCE LA English DT Article ID OLD-WORLD BLUESTEM; TALLGRASS PRAIRIE; BIG BLUESTEM; FERTILIZER; QUALITY; ATRAZINE; GRASSES; FORAGE AB Development of a biofuel industry based on biomass will require large quantities of cellulosic feedstock. Among the proposals for acquiring this feedstock, without impacting other land uses such as food or forage, is the use of up to 50% of Conservation Reserve Program (CRP) land. Our objective was to determine CRP biomass production in northwestern Oklahoma and record the harvest impact on plant species composition, plant growth, and soil characteristics. Six CRP sites were harvested on three dates, early August, early October, and postfrost, for three consecutive years, 2004, 2005, and 2006. Three sites were Old World bluestem (OWB; Bothriochloa spp.) and three sites were native mixed species (NM). Across all years and harvest dates OWB produced 3790 kg ha(-1) and NM produced 1920 kg ha(-1) of dry biomass. Maximum yields were obtained at the October harvest for both OWB (4170 kg ha(-1)) and NM (2180 kg ha(-1)). There was no observed change in species composition or soil characteristics. Among all species evaluated, nitrogen, neutral detergent fiber, carbon, and ash concentrations varied significantly. Within NM, production of dry biomass among native grass species differed and ranged from 213 g plant(-1) for big bluestem (Andropogon gerardii Vitman) to 14 g plant(-1) for sand lovegrass [Eragrostis trichodes (Nutt.) Alph. Wood]. Biomass production consistently declined at all sites and for all harvest dates over the three harvest years, but the greatest decline in yield was for OWB. C1 [Venuto, B. C.; Daniel, J. A.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Venuto, BC (reprint author), ARS, USDA, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM brad.venuto@ars.usda.gov NR 34 TC 9 Z9 9 U1 0 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 BP 737 EP 743 DI 10.2135/cropsci2008.11.0641 PG 7 WC Agronomy SC Agriculture GA 568ZE UT WOS:000275564500035 ER PT J AU Bouis, HE Welch, RM AF Bouis, Howarth E. Welch, Ross M. TI Biofortification-A Sustainable Agricultural Strategy for Reducing Micronutrient Malnutrition in the Global South SO CROP SCIENCE LA English DT Article; Proceedings Paper CT CGIAR Science Forum 2009 CY JUN 16-17, 2009 CL Wageningen, NETHERLANDS ID STAPLE FOOD CROPS; NUTRITIONAL QUALITY; IRRIGATION WATER; ZINC FERTILIZERS; IRON-DEFICIENCY; GUT MICROBIOTA; VITAMIN-A; BIOAVAILABILITY; MAIZE; BIFIDOBACTERIA AB Minerals and vitamins in food staples eaten widely by the poor may be increased either through conventional plant breeding or through use of transgenic techniques, a process known as biofortification. HarvestPlus seeks to develop and distribute cultivars of food staples (rice [Oryza sativa L.], wheat [Triticum aestivum L.], maize [Zea mays L.], cassava [Manihot esculenta Crantz], pearl millet [Pennisetum americanum Leeke], beans [Phaseolus vulgaris L.], sweet potato [Ipomoea batatas L.]) that are high in Fe, Zn, and provitamin A through an interdisciplinary global alliance of scientific institutions and implementing agencies in developing and developed countries. Biofortified crops offer a rural-based intervention that, by design, initially reaches these more remote populations, which comprise a majority of the undernourished in many countries, and then penetrates to urban populations as production surpluses are marketed. Thus, biofortification complements fortification and supplementation programs, which work best in centralized urban areas and then reach into rural areas with good infrastructure. Initial investments in agricultural research at a central location can generate high recurrent benefits at low cost as adapted biofortified cultivars; become widely available in countries across time at low recurrent costs. Overall, three things must happen for biofortification to be successful. First, the breeding must be successful-high nutrient density must be combined with high yields and high profitability. Second, efficacy must be demonstrated - the micronutrient status of human subjects must be shown to improve when consuming the biofortified cultivars as normally eaten. Third, the biofortified crops must be adopted by farmers and consumed by those suffering from micronutrient malnutrition in significant numbers. C1 [Welch, Ross M.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Bouis, Howarth E.] HarvestPlus, Int Food Policy Res Inst, Washington, DC 20006 USA. RP Welch, RM (reprint author), ARS, USDA, Robert W Holley Ctr Agr & Hlth, Tower Rd, Ithaca, NY 14853 USA. EM rmw1@cornell.edu NR 76 TC 116 Z9 122 U1 10 U2 103 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 SU 1 BP S20 EP S32 DI 10.2135/cropsci2009.09.0531 PG 13 WC Agronomy SC Agriculture GA 568ZG UT WOS:000275564700005 ER PT J AU Cakir, C Gillespie, ME Scofield, SR AF Cakir, Cahid Gillespie, Megan E. Scofield, Steven R. TI Rapid Determination of Gene Function by Virus-induced Gene Silencing in Wheat and Barley SO CROP SCIENCE LA English DT Article; Proceedings Paper CT CGIAR Science Forum 2009 CY JUN 16-17, 2009 CL Wageningen, NETHERLANDS ID STRIPE-MOSAIC-VIRUS; DISEASE-RESISTANCE GENE; POWDERY MILDEW; HEXAPLOID WHEAT; PLANTS; BIOSYNTHESIS; REPLICATION; ARABIDOPSIS; TRANSCRIPTS; SYNTHASE AB The cereal crops are essential components to the human and animal food supply. Solutions to many of the problems challenging cereal production will require identification of genes responsible for particular traits. Unfortunately, the process of identifying gene function is very slow and complex in crop plants. In wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.), this process is made very difficult by the very large size and complexity of their genomes and the difficulty with which these crops can be genetically transformed. Additionally, the polyploidy of wheat greatly complicates any approach based on mutational analysis because functional, homeologous genes often mask genetic mutations. Virus-induced gene silencing (VIGS) is an important new tool that overcomes many of these obstacles and promises to greatly facilitate the assessment of gene function. A VIGS system based on barley stripe mosaic virus (BSMV) has recently been developed for use in wheat and barley. The BSMV-VIGS system allows researchers to switch-off or "knockdown" the expression of chosen genes so that the gene's function may be inferred based on the knockout phenotypes. This article describes the characteristics of the BSMV-VIGS system, relates examples of its application for functional genomics in wheat and barley, and discusses the strengths and weaknesses of this approach. C1 [Scofield, Steven R.] ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Cakir, Cahid] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Gillespie, Megan E.] Purdue Univ, Dep Agron, W Lafayette, IN 47906 USA. RP Scofield, SR (reprint author), ARS, USDA, Crop Prod & Pest Control Res Unit, 915 W State St, W Lafayette, IN 47907 USA. EM scofield@purdue.edu RI Scofield, Steven/C-3868-2016 NR 30 TC 22 Z9 22 U1 0 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2010 VL 50 IS 2 SU 1 BP S77 EP S84 DI 10.2135/cropsci2009.10.0567 PG 8 WC Agronomy SC Agriculture GA 568ZG UT WOS:000275564700010 ER PT J AU Bonnart, R Volk, GM AF Bonnart, Remi Volk, Gayle M. TI INCREASED EFFICIENCY USING THE ENCAPSULATION-DEHYDRATION CRYOPRESERVATION TECHNIQUE FOR Arabidopsis thaliana SO CRYOLETTERS LA English DT Article DE alginate; Arabidopsis thaliana; dehydration; encapsulation; liquid nitrogen ID VITRO-GROWN MERISTEMS; VEGETATIVE BUD METHOD; SHOOT-TIPS; PLANT CRYOPRESERVATION; THERMAL-ANALYSIS; VITRIFICATION; L.; APICES; GERMPLASM; PROTOCOLS AB Arabidopsis thaliana shoot tips were successfully cryopreserved using encapsulation-dehydration cryopreservation methods. Between one and seven shoot tips were encapsulated within 4 mm calcium-alginate beads. Beads were formed in the presence of 2 M glycerol + 0.4 M sucrose. The time required to make 10 beads, each containing five shoot tips (4 min), was less than the time required to make 50 beads containing one shoot tip (12 min). Shoot tip regrowth after cryoexposure was between 60 and 68%, with one to seven shoot tips per bead. Using five Arabidopsis shoot tips per bead, alginate beads were formed either in the presence of 2 M glycerol + 0.4 M sucrose or 0.5 M sucrose. Beads formed in the presence of glycerol were immediately air-dried to moisture contents between 0.21 to 0.26 g H(2)O/g FW (0.27 to 0.38 g H(2)O/g DW). Alginate beads formed in 0.5 M sucrose were incubated in solutions of 0.5, 0.75, and 1 M sucrose for one day each prior to air-dehydration, achieving moisture contents of 0.19 to 0.21 g H(2)O/g FW (0.23 to 0.27 g H(2)O/g DW). Shoot tip regrowth after cryoexposure was between 42 and 65%, with no significant differences among treatments. We successfully reduced the amount of time needed for shoot tip processing for Arabidopsis by encapsulating five shoot tips per alginate bead and by using a glycerol-encapsulation method, without lowering shoot tip regrowth levels after cryopreservation. C1 [Bonnart, Remi; Volk, Gayle M.] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Volk, GM (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM Gayle.Volk@ars.usda.gov NR 35 TC 3 Z9 3 U1 0 U2 3 PU CRYO LETTERS PI LONDON PA C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND SN 0143-2044 J9 CRYOLETTERS JI CryoLetters PD MAR-APR PY 2010 VL 31 IS 2 BP 95 EP 100 PG 6 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA 600NA UT WOS:000277992200001 PM 20687451 ER PT J AU Uchendu, EE Reed, BM AF Uchendu, Esther E. Reed, Barbara M. TI VITAMINS C AND E IMPROVE IN VITRO RECOVERY OF CRYOPRESERVED BLACKBERRY SHOOT TIPS SO CRYOLETTERS LA English DT Meeting Abstract C1 [Uchendu, Esther E.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS, Corvallis, OR 97333 USA. EM Barbara.Reed@ars.usda.gov NR 0 TC 1 Z9 1 U1 0 U2 1 PU CRYO LETTERS PI LONDON PA C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND SN 0143-2044 J9 CRYOLETTERS JI CryoLetters PD MAR-APR PY 2010 VL 31 IS 2 BP 180 EP 180 PG 1 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA 600NA UT WOS:000277992200026 ER PT J AU Volk, GM AF Volk, Gayle M. TI Application of Functional Genomics and Proteomics to Plant Cryopreservation SO CURRENT GENOMICS LA English DT Article DE Cryobiology; cold hardiness; desiccation tolerance; stress; microarray; vitrification ID TRANSCRIPTIONAL REGULATORY NETWORKS; RESPONSIVE GENE-EXPRESSION; FATTY-ACID-COMPOSITION; THALIANA SHOOT TIPS; BANANA MUSA SPP.; ARABIDOPSIS-THALIANA; COLD-ACCLIMATION; STRESS RESPONSES; PLASMA-MEMBRANE; ISOLATED PROTOPLASTS AB Plant cryobiology has primarily emerged from the classical fields of cryobiology and plant stress physiology. Cryopreservation tools are now available to geneticists for germplasm preservation and the field itself is advancing significantly through the use of molecular techniques. Long-term preservation of vegetatively propagated tissues can minimize the risks of long-term maintenance under tissue culture or field conditions. Cells can be successfully cryopreserved when the adverse affects of ice crystal formation are mitigated by the removal of water or procedures to limit ice formation and crystal growth. The addition of cryoprotectant solutions to hydrated cells may improve the survival of microdissected shoot tips or embryonic axes. Recent discoveries in the genetic pathways leading to cold acclimation and freezing tolerance suggest the involvement of key cold-regulated genes in the acquisition of cold tolerance in plant tissues. Model systems of banana and Arabidopsis have revealed the involvement of genes and proteins in the glycolytic and other metabolic pathways, particularly processes involved in dehydration tolerance, osmoprotection, and membrane transport. Furthermore, successful recovery appears to be dependent upon the presence of antioxidant protection from reactive oxygen species. Characterization of specific genes and proteins will lead to significant advances in plant cryobiology research. C1 ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Volk, GM (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM Gayle.Volk@ars.usda.gov NR 77 TC 7 Z9 9 U1 2 U2 19 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1389-2029 J9 CURR GENOMICS JI Curr. Genomics PD MAR PY 2010 VL 11 IS 1 BP 24 EP 29 PG 6 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 545OD UT WOS:000273742500004 PM 20808520 ER PT J AU Horvath, D AF Horvath, David TI Genomics for Weed Science SO CURRENT GENOMICS LA English DT Article ID SINGLE-NUCLEOTIDE MUTATIONS; SPURGE EUPHORBIA-ESULA; LEAFY SPURGE; GENE-EXPRESSION; LOLIUM-PERENNE; ARABIDOPSIS MICROARRAYS; MEDIATED TRANSFORMATION; ALLIARIA-PETIOLATA; INVASIVE PLANT; WILD AB Numerous genomic-based studies have provided insight to the physiological and evolutionary processes involved in developmental and environmental processes of model plants such as arabidopsis and rice. However, far fewer efforts have been attempted to use genomic resources to study physiological and evolutionary processes of weedy plants. Genomics-based tools such as extensive EST databases and microarrays have been developed for a limited number of weedy species, although application of information and resources developed for model plants and crops are possible and have been exploited. These tools have just begun to provide insights into the response of these weeds to herbivore and pathogen attack, survival of extreme environmental conditions, and interaction with crops. The potential of these tools to illuminate mechanisms controlling the traits that allow weeds to invade novel habitats, survive extreme environments, and that make weeds difficult to eradicate have potential for both improving crops and developing novel methods to control weeds. C1 ARS, USDA, Biosci Res Lab, Fargo, ND 58105 USA. RP Horvath, D (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58105 USA. EM David.horvath@ars.usda.gov OI Horvath, David/0000-0002-8458-7691 NR 59 TC 2 Z9 2 U1 0 U2 6 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y26, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1389-2029 J9 CURR GENOMICS JI Curr. Genomics PD MAR PY 2010 VL 11 IS 1 BP 47 EP 51 PG 5 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 545OD UT WOS:000273742500007 PM 20808523 ER PT J AU Smith, CE Ordovas, JM AF Smith, Caren E. Ordovas, Jose M. TI Fatty acid interactions with genetic polymorphisms for cardiovascular disease SO CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE LA English DT Article DE cardiovascular disease; fatty acids; nutrient-gene interactions ID HEPATIC LIPASE GENE; RANDOMIZED CLINICAL-TRIAL; 2 HYPOCALORIC DIETS; MYOCARDIAL-INFARCTION; METABOLIC SYNDROME; CHOLESTEROL CONCENTRATIONS; PRO12ALA POLYMORPHISM; 5-LIPOXYGENASE GENE; INSULIN SENSITIVITY; ARACHIDONIC-ACID AB Purpose of review The number of studies investigating interactions between genes and nutrients for cardiovascular disease continues to grow, and holds tremendous potential for reducing disease risk at the level of the individual genotype. However, understanding the limitations and challenges of interaction studies, whether of observational or interventional design, is essential for critical evaluation of these studies. Recent findings Nutrient-gene interactions for cardiovascular disease both parallel and extend nutrition studies, encompassing both traditional and novel cardiovascular risk factors. Fatty acid quality, lipid metabolism, inflammation, postprandial metabolism, fatty liver and macronutrient-gene interactions for obesity and metabolic syndrome represent a subset of the major areas of recent focus. With few exceptions, however, studies of gene-nutrient interactions are limited to a single population. Summary Gene-nutrient research will continue to expand as genome-wide association studies uncover new sources of genetic variability associated with cardiovascular risk. However, in addition to investigation of newly discovered variants, continuing efforts must focus on the confirmation of previously reported genetic associations and interactions in additional populations. C1 [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA, HNRCA, Boston, MA 02111 USA. RP Ordovas, JM (reprint author), Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA, HNRCA, 711 Washington St, Boston, MA 02111 USA. EM jose.ordovas@tufts.edu OI Ordovas, Jose/0000-0002-7581-5680 FU NHLBI NIH HHS [R01 HL054776-12, R01 HL054776, HL54776]; NIA NIH HHS [5P01AG023394, P01 AG023394]; NIDDK NIH HHS [DK075030, R01 DK075030, R01 DK075030-03, T32 DK007651, T32 DK007651-19]; NIMHD NIH HHS [L60 MD003562] NR 46 TC 11 Z9 11 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1363-1950 J9 CURR OPIN CLIN NUTR JI Curr. Opin. Clin. Nutr. Metab. Care PD MAR PY 2010 VL 13 IS 2 BP 139 EP 144 DI 10.1097/MCO.0b013e3283357287 PG 6 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 562OX UT WOS:000275063200004 PM 19996744 ER PT J AU Kim, CH Lillehoj, HS Hong, YH Keeler, CL Lillehoj, EP AF Kim, Chul-Hong Lillehoj, Hyun S. Hong, Yeong-Ho Keeler, Calvin L., Jr. Lillehoj, Erik P. TI Comparison of global transcriptional responses to primary and secondary Eimeria acervulina infections in chickens SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Coccidiosis; Intestinal immune response; Microarray; Primary and secondary infections; Avian intestinal intraepithelial lymphocyte; cDNA microarray (AVIELA) ID DIFFERENTIALLY EXPRESSED GENES; MAXIMA INFECTION; CDNA MICROARRAY; COCCIDIOSIS; VACCINATION; DESIGN; GUT AB In the current study, we compared chicken gene transcriptional profiles following primary and secondary infections with Eimeria acervulina using a 9.6K avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA). Gene Ontology analysis showed that primary infection significantly modulated the levels of mRNAs for genes involved in the metabolism of lipids and carbohydrates as well as those for innate immune-related genes. By contrast, secondary infection increased the levels of transcripts encoded by genes related to humoral immunity and reduced the levels of transcripts for the innate immune-related genes. Because the observed modulation in transcript levels for gene related to energy metabolism and immunity occurred concurrent with the clinical signs of coccidiosis, these results suggest that altered expression of a specific set of host genes induced by Eimeria infection may be responsible, in part, for the observed reduction in body weight gain and inflammatory gut damage that characterizes avian coccidiosis. Published by Elsevier Ltd. C1 [Kim, Chul-Hong; Lillehoj, Hyun S.; Hong, Yeong-Ho] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Keeler, Calvin L., Jr.] Univ Delaware, Coll Agr & Nat Resources, Dept Anim & Food Sci, Newark, DE 19716 USA. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 20201 USA. RP Lillehoj, HS (reprint author), USDA ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Bldg 1040,BARC E, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ARS.USDA.GOV NR 22 TC 5 Z9 5 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD MAR PY 2010 VL 34 IS 3 BP 344 EP 351 DI 10.1016/j.dci.2009.11.006 PG 8 WC Immunology; Zoology SC Immunology; Zoology GA 559NR UT WOS:000274832700014 PM 19941894 ER PT J AU Mao, WF Hunt, HD Cheng, HH AF Mao, Weifeng Hunt, Henry D. Cheng, Hans H. TI Cloning and functional characterization of chicken stem cell antigen 2 SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Stem cell antigen 2 (SCA2); Chicken; Antibody; Bursa of Fabricius; Cortical-medullary epithelial cells (CMEC); B cells ID THYMIC SHARED ANTIGEN-1; GENE-EXPRESSION; SELF-RENEWAL; ERYTHROCYTIC PROGENITORS; SIGNAL-TRANSDUCTION; LY-6 FAMILY; FABRICIUS; IMMATURE; DISEASE; MEMBER AB Stem cell antigen 2 (SCA2) is a Ly6 family member whose function is largely unknown. To characterize biological properties and tissue distribution of chicken SCA2, SCA2 was expressed in E. coli, purified, and a polyclonal antibody developed. Utilizing the polyclonal antibody, SCA2 is a 13 kDa cell surface protein anchored by a glycosyl-phosphatidylinositol (GPI) moiety. SCA2 is expressed in connective tissues of thymus and bursa based on immunohistochemistry, immunoprecipitation, and western blots. In bursal follicles, SCA2 is specifically expressed on the cortical-medullary epithelia[ cells (CMEC) surrounded by MHC class II presenting cells. Expression profiles of bursal cells induced by contact with SCA2-expressing cells shows down-regulation of numerous genes including CD79B, B cell linker (BLNK), spleen tyrosine kinase (SYK), and gamma 2-phospholipase C (PLCG2) that are involved in the B cell receptor (BCR) and immune response signaling pathways. These results suggest chicken SCA2 plays a role in regulating B lymphocytes. Published by Elsevier Ltd. C1 [Mao, Weifeng; Hunt, Henry D.; Cheng, Hans H.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Mao, Weifeng] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. RP Cheng, HH (reprint author), ARS, USDA, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM hans.cheng@ars.usda.gov FU USDA NRICGP [200305414] FX We thank L. Molitor and N. Koller for technical support, and A.S. Porter, K.A. Joseph, and R.A. Rosebury for their assistance with immunohistochemistry, which was carried out at the Investigative Histophathology Laboratory, Department of Physiology/Division of Human Pathology, Michigan State University. We thank J. Landgraf and A. Thelen for their assistance with the Affymetrix array experiment, which was carried out at Research Technology Support Facility, Michigan State University. We also thank Dr. Heidari for technical assistance with real-time PCR. This work was supported in part by the USDA NRICGP grant #200305414. NR 42 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD MAR PY 2010 VL 34 IS 3 BP 360 EP 368 DI 10.1016/j.dci.2009.11.008 PG 9 WC Immunology; Zoology SC Immunology; Zoology GA 559NR UT WOS:000274832700016 PM 19945479 ER PT J AU Hsieh, J Longuet, C Baker, CL Qin, B Federico, LM Drucker, DJ Adeli, K AF Hsieh, J. Longuet, C. Baker, C. L. Qin, B. Federico, L. M. Drucker, D. J. Adeli, K. TI The glucagon-like peptide 1 receptor is essential for postprandial lipoprotein synthesis and secretion in hamsters and mice SO DIABETOLOGIA LA English DT Article DE Dipeptidyl peptidase-4; Incretins; Lipids; Triacylglycerol ID DIPEPTIDYL PEPTIDASE-4 INHIBITOR; INSULIN-RESISTANCE; PARTICLE CHOLESTEROL; GLUCOSE-HOMEOSTASIS; ENERGY-EXPENDITURE; ANIMAL-MODEL; DOUBLE-BLIND; PROTEIN; POLYPEPTIDE; SITAGLIPTIN AB Glucagon-like peptide 1 (GLP-1) receptor (GLP-1R) agonists and dipeptidyl peptidase-4 (DPP-4) inhibitors attenuate postprandial lipaemia through mechanisms that remain unclear. As dyslipidaemia is a contributing risk factor for cardiovascular disease in type 2 diabetes, we examined the mechanisms linking pharmacological and physiological regulation of GLP-1 action to control of postprandial lipid metabolism. Postprandial lipid synthesis and secretion were assessed in normal and fructose-fed hamsters and in wild-type mice that were treated with or without sitagliptin. Apolipoprotein B-48 (ApoB-48) synthesis and secretion were also examined in primary enterocyte cultures. The importance of exogenous vs endogenous GLP-1R signalling for regulation of intestinal lipoprotein synthesis and secretion was assessed in mice and hamsters treated with the GLP-1R agonist exendin-4, the GLP-1R antagonist exendin(9-39) and in Glp1r (+/+) vs Glp1r (-/-) mice. Sitagliptin decreased fasting plasma triacylglycerol, predominantly in the VLDL fraction, as well as postprandial triacylglycerol-rich lipoprotein (TRL)-triacylglycerol, TRL-cholesterol and TRL-ApoB-48 in hamsters and mice. GLP-1R activation with exendin-4 alone also decreased plasma and TRL-ApoB-48 in hamsters and mice, and reduced secretion of ApoB-48 in hamster enterocyte cultures. Conversely, blockade of endogenous GLP-1R signalling by the antagonist exendin(9-39) or genetic elimination of GLP-1R signalling in Glp1r (-/-) mice enhanced TRL-ApoB-48 secretion in vivo. Co-administration of exendin(9-39) also abolished the hypolipidaemic effect of sitagliptin. Potentiation of endogenous incretin action via DPP-4 inhibition or pharmacological augmentation of GLP-1R signalling reduces intestinal secretion of triacylglycerol, cholesterol and ApoB-48. Moreover, endogenous GLP-1R signalling is essential for the control of intestinal lipoprotein biosynthesis and secretion. C1 [Drucker, D. J.] Mt Sinai Hosp SLRI, Toronto, ON M5G 1X5, Canada. [Hsieh, J.; Adeli, K.] Univ Toronto, Dept Biochem, Toronto, ON, Canada. [Hsieh, J.; Baker, C. L.; Federico, L. M.; Adeli, K.] Univ Toronto, Hosp Sick Children, Res Inst, Toronto, ON M5G 1X8, Canada. [Longuet, C.; Drucker, D. J.] Univ Toronto, Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Dept Med, Toronto, ON M5G 1X5, Canada. [Qin, B.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Drucker, DJ (reprint author), Mt Sinai Hosp SLRI, 600 Univ Ave TCP5-1004, Toronto, ON M5G 1X5, Canada. EM d.drucker@utoronto.ca RI Drucker, Daniel/A-4092-2010 FU CIHR [MOP-53093, MOP-82700]; Canada Research Chairs Program FX This work was supported in part by CIHR operating grants MOP-53093 (to K. Adeli) and MOP-82700 (to D. J. Drucker), and by the Canada Research Chairs Program (D. J. Drucker). NR 32 TC 111 Z9 120 U1 1 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0012-186X J9 DIABETOLOGIA JI Diabetologia PD MAR PY 2010 VL 53 IS 3 BP 552 EP 561 DI 10.1007/s00125-009-1611-5 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 551VG UT WOS:000274238500021 PM 19957161 ER PT J AU Laaksonen, S Pusenius, J Kumpula, J Venalainen, A Kortet, R Oksanen, A Hoberg, E AF Laaksonen, Sauli Pusenius, Jyrki Kumpula, Jouko Venalainen, Ari Kortet, Raine Oksanen, Antti Hoberg, Eric TI Climate Change Promotes the Emergence of Serious Disease Outbreaks of Filarioid Nematodes SO ECOHEALTH LA English DT Article DE global warming; filarioid nematodes; vector-borne diseases; emerging diseases; reindeer populations; sub-Arctic ID REINDEER RANGIFER-TARANDUS; SETARIA-TUNDRA; INFECTIOUS-DISEASES; PARASITIC DISEASES; PERITONITIS; PATHOGENS; DYNAMICS; WILDLIFE; IMPACTS; ECOLOGY AB Filarioid parasites represent major health hazards with important medical, veterinary, and economic implications, and considerable potential to affect the everyday lives of tens of millions of people globally (World Health Organization, 2007). Scenarios for climate change vary latitudinally and regionally and involve direct and indirect linkages for increasing temperature and the dissemination, amplification, and invasiveness of vector-borne parasites. High latitude regions are especially influenced by global climate change and thus may be prone to altered associations and dynamics for complex host-pathogen assemblages and emergence of disease with cascading effects on ecosystem structure. Although the potential for substantial ecological perturbation has been identified, few empirical observations have emanated from systems across the Holarctic. Coincidental with decades of warming, and anomalies of high temperature and humidity in the sub-Arctic region of Fennoscandia, the mosquito-borne filarioid nematode Setaria tundra is now associated with emerging epidemic disease resulting in substantial morbidity and mortality for reindeer and moose. We describe a host-parasite system that involves reindeer, arthropods, and nematodes, which may contribute as a factor to ongoing declines documented for this ungulate species across northern ecosystems. We demonstrate that mean summer temperatures exceeding 14A degrees C drive the emergence of disease due to S. tundra. An association between climate and emergence of filarioid parasites is a challenge to ecosystem services with direct effects on public health, sustainability of free-ranging and domestic ungulates, and ultimately food security for subsistence cultures at high latitudes. C1 [Laaksonen, Sauli; Oksanen, Antti] Finnish Food Safety Author Evira FINPAR, FI-90101 Oulu, Finland. [Pusenius, Jyrki] Finnish Game & Fisheries Res Inst, FI-80100 Joensuu, Finland. [Kumpula, Jouko] Finnish Game & Fisheries Res Inst, FI-99910 Kaamanen, Finland. [Venalainen, Ari] Finnish Meteorol Inst, FI-00101 Helsinki, Finland. [Kortet, Raine] Univ Eastern Finland, Dept Biol, FI-80101 Joensuu, Finland. [Hoberg, Eric] ARS, US Natl Parasite Collect, USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Laaksonen, S (reprint author), Finnish Food Safety Author Evira FINPAR, POB 517, FI-90101 Oulu, Finland. EM sauli.laaksonen@evira.fi RI Kortet, Raine/J-5027-2012 OI Kortet, Raine/0000-0003-3749-1096 NR 44 TC 32 Z9 35 U1 7 U2 59 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 J9 ECOHEALTH JI EcoHealth PD MAR PY 2010 VL 7 IS 1 BP 7 EP 13 DI 10.1007/s10393-010-0308-z PG 7 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 634PP UT WOS:000280595700002 PM 20422252 ER PT J AU Potts, DL Scott, RL Bayram, S Carbonara, J AF Potts, Daniel L. Scott, Russell L. Bayram, Saziye Carbonara, Joaquin TI Woody plants modulate the temporal dynamics of soil moisture in a semi-arid mesquite savanna SO ECOHYDROLOGY LA English DT Article DE Prosopis velutina; soil moisture; fast Fourier transform; Santa Rita Experimental Range; time domain reflectometry ID SONORAN DESERT; PRECIPITATION PULSE; CHIHUAHUAN DESERT; GAS-EXCHANGE; GRASSLAND; WATER; VARIABILITY; SHRUBLAND; ECOSYSTEM; CARBON AB Climate variability and human activities interact to increase the abundance of woody plants in arid and semi-arid ecosystems worldwide. How woody plants interact with rainfall to influence patterns of soil Moisture through time, at different depths in the soil profile and between neighboring landscape patches is poorly known. In a semi-arid mesquite savanna we deployed a paired array of sensors beneath a mesquite canopy and in an adjacent open area to measure volumetric soil Water content (theta) every 30 min at several depths between 2004 and 2007. In addition, to quantify temporally dynamic variation in soil moisture between the two microsites and across soil depths we analysed theta time-series using fast Fourier transforms (FFT). FFT analyses were consistent with the prediction that by reducing evaporative losses through shade and reducing rainfall inputs through canopy interception of small rainfall events, the mesquite canopy was associated with a decline in high-frequency (hour-to-hour and day-to-day) variation in shallow theta. Finally, we found that, in both microsites, high-frequency theta variation declined with increasing soil depth as the influence of evaporative losses and inputs associated with smaller rainfall events declined. In this case, we argue that the buffering of shallow soil moisture against high-frequency variations can enhance nutrient cycling and alter the carbon cycle in dryland ecosystems. Copyright (C) 2009 John Wiley & Sons, Ltd. C1 [Potts, Daniel L.] SUNY Coll Buffalo, Dept Biol, Buffalo, NY 14222 USA. [Scott, Russell L.] USDA ARS, SW Watershed Res Ctr, Tucson, AZ 85719 USA. [Bayram, Saziye; Carbonara, Joaquin] SUNY Coll Buffalo, Dept Math, Buffalo, NY 14222 USA. RP Potts, DL (reprint author), SUNY Coll Buffalo, Dept Biol, Buffalo, NY 14222 USA. EM pottsdl@buffalostate.edu FU USDA-ARS; National Science Foundation [EAR-9876800]; Buffalo State College; University of Arizona FX This work was supported in part by the USDA-ARS, Sustainability of semi-Arid Hydrology and Riparian Areas (SAHRA) under the STC Program of the National Science Foundation, Agreement No. EAR-9876800 and Buffalo State College. Rainfall data were provided by the Santa Rita Experimental Range Digital Database which is supported by the University of Arizona. K. Delp, J. Manning and D. Ettestad provided important insight in the analysis of soil moisture data and the interpretation of Fast Fourier Transforms. We gratefully acknowledge the support and contributions of W. Cable, N. English, E. Hamerlynck, M. Heitlinger, T. Huxman, M. McClaran and the thoughtful comments of two anonymous reviewers. NR 49 TC 22 Z9 24 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 J9 ECOHYDROLOGY JI Ecohydrology PD MAR PY 2010 VL 3 IS 1 BP 20 EP 27 DI 10.1002/eco.91 PG 8 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 572DZ UT WOS:000275808800003 ER PT J AU Duniway, MC Snyder, KA Herrick, JE AF Duniway, Michael C. Snyder, Keirith A. Herrick, Jeffrey E. TI Spatial and temporal patterns of water availability in a grass-shrub ecotone and implications for grassland recovery in arid environments SO ECOHYDROLOGY LA English DT Article DE desertification; petrocalcic; soil water; available water; Prosopis; Bouteloua; Chihuahuan desert ID SOUTHERN NEW-MEXICO; CHIHUAHUAN DESERT; SEMIARID WOODLAND; COMPUTER-PROGRAM; SOIL-MOISTURE; HETEROGENEITY; ECOSYSTEMS; MESQUITE; PRECIPITATION; CONDUCTIVITY AB Encroachment of woody shrubs into historic desert grasslands is a major problem throughout the world. Conversion of grasslands to shrub-dominated systems may result in significant alteration of biogeochemical processes and reduced resource availability in shrub interspaces, making grassland recovery difficult. Soil petrocalcic horizons. which are common in arid and semi-arid regions globally, have been shown to dramatically alter plant water availability. To assess how soil water dynamics are affected v woody encroachment in petrocalcic soils under contrasting precipitation patterns, We conducted a 3-year replicated study in a mixed shrub-grass system measuring soil water in unvegetated interspaces and under mesquite canopies. Plots were instrumented with time-domain reflectometry (TDR) Moisture probes, both above and within the soil petrocalcic horizon. Soils under both cover types maintained large increases in available water content for several months during a wetter than normal winter and summer (increases of 0.08-0.16 m(3) m(-3)). Interspace soils absorbed significantly greater quantities of water during the winter and retained more water into the spring than Soils Under shrubs. In contrast. soils under shrubs initially absorbed greater volumes of water during and following summer rains. Differing seasonal dynamics were attributed to interactions between the unique properties of soils with petrocalcic horizons and canopy-induced variability in evapotranspiration. Observed patterns of plant-available water do not Support the hypothesis of greater resource availability under shrubs. Similar or greater water availability in shrub interspaces indicates that concentration of soil Water under shrubs may not be a process limiting grass recovery oil these soils. Published in 2010 by John Wiley & Soils, Ltd. C1 [Duniway, Michael C.; Herrick, Jeffrey E.] USDA ARS, MSC 3JER, Las Cruces, NM 88003 USA. [Snyder, Keirith A.] USDA ARS, Reno Unit, Reno, NV 89512 USA. RP Duniway, MC (reprint author), USDA ARS Jornada Expt Range, MSC 3JER, POB 30003, Las Cruces, NM 88003 USA. EM mduniway@nmsu.edu OI Duniway, Michael/0000-0002-9643-2785 FU National Science Foundation [08 010 209 DEB]; USDA-ARS Jornada Experimental Range and T E Inc FX Many thanks are due to Steve Evett for his technical help with the TDR system, to the Jornada Experimental Range staff for field help and logistical support, to Marta Remmenga for statistical help and to two anonymous reviewers. This work was supported by the National Science Foundation grant to New Mexico State University for the Jornada Basin Long Term Ecological Research program (08 010 209 DEB), the USDA-ARS Jornada Experimental Range and T & E Inc. NR 58 TC 21 Z9 22 U1 5 U2 36 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1936-0584 J9 ECOHYDROLOGY JI Ecohydrology PD MAR PY 2010 VL 3 IS 1 BP 55 EP 67 DI 10.1002/eco.94 PG 13 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 572DZ UT WOS:000275808800006 ER PT J AU Stein, CM Johnson, DW Miller, WW Powers, RF Young, DA Glass, DW AF Stein, C. M. Johnson, D. W. Miller, W. W. Powers, R. F. Young, D. A. Glass, D. W. TI Snowbrush (Ceanothus velutinus Dougl) effects on nitrogen availability in soils and solutions from a Sierran ecosystem SO ECOHYDROLOGY LA English DT Article DE snowbrush; nitrogen fixation; nitrate; ammonium; soil solution; runoff; resin lysimeters ID ALDER-CONIFER STANDS; RED ALDER; ADJACENT CONIFER; PINE STANDS; LAKE TAHOE; FOREST; GROWTH; FIR; WATERSHEDS; CALIFORNIA AB The presence of nitrogen (N) fixing vegetation can have significant deleterious effects on water quality and also soil leaching This study investigates the effects of snowbrush (Ceanothus velutinus Dougl.), a common post-disturbance N fixer in the western United States. on water quality and soil leaching. We compared mineral N concentrations in soil Solution. runoff. O horizon leachates and resin lysimeters in adjacent snowbrush and Jeffrey pine (Pinus jeffreyii Grev. & Balf) stands in one site in the eastern Sierra Nevada Mountains in Nevada. Soil Solutions from snowbrush had slightly but not significantly greater NO(3)(-) Concentrations than those from Jeffrey pine. Leaching rates of NO(3)(-) and NH(4)(+) measured by resin lysimeters were lower in snowbrush than in Jeffrey pine, and leaching rates in mineral soil (15 cm) were not significantly different. Nitrate and NH(4)(+) concentrations in runoff were greater in the snowbrush than Jeffrey pine, and ancillary field column studies confirmed that snowbrush litter was the primary source of this elevated mineral N. Cutting, followed by mastication and herbicide treatments had no effect oil mineral N leaching in a snowbrush stand growing oil a recently burned (1994) site in the eastern Sierra Nevada Mountains of California. We conclude from this and previous studies that snowbrush. unlike some other post-disturbance N-fixing species. has minimal and inconsistent effects on water quality in the eastern Sierra Nevada Mountains. Copyright (C) 2009 John Wiley, & Sons, Ltd. C1 [Stein, C. M.; Johnson, D. W.; Miller, W. W.; Glass, D. W.] Univ Nevada, Reno, NV 89557 USA. [Powers, R. F.] US Forest Serv, Pacific SW Res Stn, USDA, Redding, CA 96002 USA. RP Johnson, DW (reprint author), Univ Nevada, Reno, NV 89557 USA. EM dwj@cabnr.unr.edu FU U.S. Forest Service; Whittell Board of Control; Nevada Agricultural Experiment Station, University of Nevada, Reno, Nevada FX This research was supported by the U.S. Forest Service, the Whittell Board of Control, and by Nevada Agricultural Experiment Station, University of Nevada, Reno, Nevada. Technical Assistance by James Murphy, Damien Domini, Georganne Stein, Dallas Glass, Dawn Hanseder and Matt Church is greatly appreciated. NR 36 TC 4 Z9 4 U1 0 U2 3 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 1936-0584 J9 ECOHYDROLOGY JI Ecohydrology PD MAR PY 2010 VL 3 IS 1 BP 79 EP 87 DI 10.1002/eco.96 PG 9 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 572DZ UT WOS:000275808800008 ER PT J AU Colunga-Garcia, M Magarey, RA Haack, RA Gage, SH Qi, JQ AF Colunga-Garcia, Manuel Magarey, Roger A. Haack, Robert A. Gage, Stuart H. Qi, Jiaquo TI Enhancing early detection of exotic pests in agricultural and forest ecosystems using an urban-gradient framework SO ECOLOGICAL APPLICATIONS LA English DT Article DE agricultural plant pests; exotic species; forest plant pests; gradient analysis; invasion risk; nonindigenous species; urban influence ID UNITED-STATES; INVASIONS; TRADE; ECOLOGY; PATHWAY; NEEDS AB Urban areas arc hubs of international transport and therefore are major gateways for exotic pests. Applying an urban gradient to analyze this pathway could provide insight into the ecological processes involved in human-mediated invasions. We defined an urban gradient for agricultural and forest ecosystems in the contiguous United States to (I) assess whether ecosystems nearer more urbanized areas were at greater risk of invasion, and (2) apply this knowledge to enhance early detection of exotic pests. We defined the gradient using the tonnage of imported products in adjacent urban areas and their distance to nearby agricultural or forest land. County-level detection reports for 39 exotic agricultural and forest pests of major economic importance were used to characterize invasions along the gradient. We found that counties with more exotic pests were nearer the urban end of the gradient. Assuming that the exotic species we analyzed represent typical invaders, then early detection efforts directed at 21-26% of U.S. agricultural and forest land would likely be able to detect 70% of invaded counties and 90% of the selected species. Applying an urban-gradient framework to current monitoring strategies should enhance early detection efforts of exotic pests, facilitating optimization in allocating resources to areas at greater risk of future invasions. C1 [Colunga-Garcia, Manuel; Qi, Jiaquo] Michigan State Univ, Ctr Global Change & Earth Observat, E Lansing, MI 48823 USA. [Magarey, Roger A.] N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. [Haack, Robert A.] US Forest Serv, USDA, No Res Stn, E Lansing, MI 48823 USA. [Gage, Stuart H.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. RP Colunga-Garcia, M (reprint author), Michigan State Univ, Ctr Global Change & Earth Observat, Manly Miles Bldg 1405 S Harrison, E Lansing, MI 48823 USA. EM colunga@msu.edu FU USDA/CSREES/Plant Biosecurity Program [2006-55605-16658] FX We thank Laura Bix, Gibbs Smith, and Robert Griffin for reviewing this manuscript. We also thank two anonymous reviewers for their suggestions to improve this manuscript. This work was supported by the USDA/CSREES/Plant Biosecurity Program grant 2006-55605-16658. NR 20 TC 12 Z9 13 U1 0 U2 19 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD MAR PY 2010 VL 20 IS 2 BP 303 EP 310 DI 10.1890/09-0193.1 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 582YA UT WOS:000276635600001 PM 20405789 ER PT J AU Faaborg, J Holmes, RT Anders, AD Bildstein, KL Dugger, KM Gauthreaux, SA Heglund, P Hobson, KA Jahn, AE Johnson, DH Latta, SC Levey, DJ Marra, PP Merkord, CL Nol, E Rothstein, SI Sherry, TW Sillett, TS Thompson, FR Warnock, N AF Faaborg, John Holmes, Richard T. Anders, Angela D. Bildstein, Keith L. Dugger, Katie M. Gauthreaux, Sidney A., Jr. Heglund, Patricia Hobson, Keith A. Jahn, Alex E. Johnson, Douglas H. Latta, Steven C. Levey, Douglas J. Marra, Peter P. Merkord, Christopher L. Nol, Erica Rothstein, Stephen I. Sherry, Thomas W. Sillett, T. Scott Thompson, Frank R., III Warnock, Nils TI Conserving migratory land birds in the New World: Do we know enough? SO ECOLOGICAL APPLICATIONS LA English DT Article DE breeding season management; conservation; en route bird conservation; global climate change; intratropical migration; land birds; migration; Partners in Flight; source-sink dynamics; winter population limitation ID NORTH-AMERICAN BIRDS; WINTER SITE FIDELITY; CLIMATE-CHANGE; HABITAT USE; NEOTROPICAL MIGRANT; NONBREEDING SEASON; GRASSLAND BIRDS; REGENERATING CLEARCUTS; POPULATION LIMITATION; SETOPHAGA-RUTICILLA AB Migratory bird needs must be met during four phases of the year: breeding season, fall migration, wintering, and spring migration; thus, management may be needed during all four phases. The bulk of research and management has focused on the breeding season, although several issues remain unsettled, including the spatial extent of habitat influences on fitness and the importance of habitat on the breeding grounds used after breeding. Although detailed investigations have shed light on the ecology and population dynamics of a few avian species, knowledge is sketchy for most species. Replication of comprehensive studies is needed for multiple species across a range of areas. Information deficiencies are even greater during the wintering season, when birds require sites that provide security and food resources needed for survival and developing nutrient reserves for spring migration and, possibly, reproduction. Research is needed on many species simply to identify geographic distributions, wintering sites, habitat use, and basic ecology. Studies are complicated, however, by the mobility of birds and by sexual segregation during winter. Stable-isotope methodology has offered an opportunity to identify linkages between breeding and wintering sites, which facilitates understanding the complete annual cycle of birds. The twice-annual migrations are the poorest-understood events in a bird's life. Migration has always been a risky undertaking, with such anthropogenic features as tall buildings, towers, and wind generators adding to the risk. Species such as woodland specialists migrating through eastern North America have numerous options for pausing during migration to replenish nutrients, but some species depend on limited stopover locations. Research needs for migration include identifying pathways and timetables of migration, quality and distribution of habitats, threats posed by towers and other tall structures, and any bottlenecks for migration. Issues such as human population growth, acid deposition, climate change, and exotic diseases arc global concerns with uncertain consequences to migratory birds and even less-certain remedies. Despite enormous gaps in our understanding of these birds, research, much of it occurring in the past 30 years, has provided sufficient information to make intelligent conservation efforts but needs to expand to handle future challenges. C1 [Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Holmes, Richard T.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Anders, Angela D.] Clancy Environm Consultants, Honolulu, HI 96825 USA. [Bildstein, Keith L.] Acopian Ctr Conservat Learning, Orwigsburg, PA 17961 USA. [Dugger, Katie M.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Gauthreaux, Sidney A., Jr.] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA. [Heglund, Patricia] US Fish & Wildlife Serv, La Crosse, WI 54603 USA. [Hobson, Keith A.] Environm Canada, Saskatoon, SK S7N 3H5, Canada. [Jahn, Alex E.; Levey, Douglas J.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Johnson, Douglas H.] USGS, No Prairie Wildlife Res Ctr, St Paul, MN 55108 USA. [Latta, Steven C.] Natl Aviary, Dept Conservat & Field Res, Pittsburgh, PA 15212 USA. [Marra, Peter P.; Sillett, T. Scott] Natl Zool Pk, Smithsonian Migratory Bird Ctr, Washington, DC 20013 USA. [Merkord, Christopher L.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Nol, Erica] Trent Univ, Ecol & Conservat Grp, Environm & Life Sci Grad Program, Peterborough, ON K9J 7B8, Canada. [Nol, Erica] Trent Univ, Dept Biol, Peterborough, ON K9J 7B8, Canada. [Rothstein, Stephen I.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Sherry, Thomas W.] Tulane Univ, Dept Ecol & Evolut Biol, New Orleans, LA 70118 USA. [Thompson, Frank R., III] Univ Missouri, USDA, US Forest Serv, No Res Stn, Columbia, MO 65211 USA. [Warnock, Nils] PRBO Conservat Sci, Petaluma, CA 94954 USA. RP Faaborg, J (reprint author), Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. EM faaborgj@missouri.edu OI Merkord, Chris/0000-0003-2825-1904 NR 171 TC 117 Z9 122 U1 9 U2 144 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD MAR PY 2010 VL 20 IS 2 BP 398 EP 418 DI 10.1890/09-0397.1 PG 21 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 582YA UT WOS:000276635600007 PM 20405795 ER PT J AU Hallam, TG Raghavan, A Kolli, H Dimitrov, DT Federico, P Qi, HR McCracken, GF Betke, M Westbrook, JK Kennard, K Kunz, TH AF Hallam, Thomas G. Raghavan, Aruna Kolli, Haritha Dimitrov, Dobromir T. Federico, Paula Qi, Hairong McCracken, Gary F. Betke, Margrit Westbrook, John K. Kennard, Kimberly Kunz, Thomas H. TI Dense and sparse aggregations in complex motion: Video coupled with simulation modeling SO ECOLOGICAL COMPLEXITY LA English DT Article DE Individual-based models; Rule-based models; Ecological censusing; Brazilian free-tailed bats (Tadarida brasiliensis); Noctuid moths ID FREE-TAILED BATS; DIETARY VARIATION AB Investigations into the complex behaviors of aggregations of highly mobile animals have not used the link between image processing technology and simulation modeling fruitfully to address many fundamental ecological issues. Examples include population censusing, which remains difficult despite the demonstrated ecological importance of assessing abundance and density of organisms. We introduce a theoretical framework that connects thermal infrared video imaging with an individual-based simulation model an approach that appears to be applicable to a diverse set of aggregated, highly mobile, nocturnal animals. To demonstrate the framework two applications are presented. The first is a dense aggregation of Brazilian free-tailed bats (Tadarida brasiliensis) that exhibits an emergence pattern that has complex dynamics and the second is a sparse local aggregation of agricultural pest moths whose dynamics are insipid. The first application uses individual-based modeling to mimic the behavior in the video of bats during a nightly emergence from a cave and to provide reliable estimates of the numbers, and associated error bounds. The second application uses video recordings of sparse aggregations to provide consistent estimates of the numbers of flying noctuid moths recorded over a corn and cotton-dominated agroecosystem in south-central Texas. This does not use a mathematical model because error estimates tend to be small. (C) 2009 Elsevier B.V. All rights reserved. C1 [Hallam, Thomas G.; Raghavan, Aruna; Kolli, Haritha; Dimitrov, Dobromir T.; Federico, Paula; McCracken, Gary F.; Kennard, Kimberly] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Raghavan, Aruna; Kolli, Haritha; Qi, Hairong] Univ Tennessee, Dept Elect Engn & Comp Sci, Knoxville, TN 37996 USA. [Betke, Margrit] Boston Univ, Dept Comp Sci, Boston, MA 02215 USA. [Westbrook, John K.] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. [Kunz, Thomas H.] Boston Univ, Dept Biol, Ctr Ecol & Conservat Biol, Boston, MA 02215 USA. RP Hallam, TG (reprint author), Univ Tennessee, Dept Ecol & Evolutionary Biol, 569 Dabney Hall, Knoxville, TN 37996 USA. EM thallam@utk.edu; Raghavan.Aruna@mayo.edu; haritha.kolli@gmail.com; ddimitro@scharp.org; pfederic@utk.edu; hqi@utk.edu; gmccrack@utk.edu; betke@bu.edu; John.Westbrook@ars.usda.gov; kkennard@utk.edu; kunz@bu.edu OI Dimitrov, Dobromir/0000-0002-2842-5436; McCracken, Gary/0000-0002-2493-8103 FU National Science Foundation [ITR-0326483]; US Environmental Protection Agency [CR 83214801] FX The National Science Foundation grant ITR-0326483 (to T.H. Kunz, G.F. McCracken, M. Betke, J.K. Westbrook, and P.A. Morton) and the Cooperative Agreement CR 83214801 (to T.G. Hallam and G.F. McCracken) with the US Environmental Protection Agency provided funding for this study. NR 26 TC 3 Z9 3 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1476-945X J9 ECOL COMPLEX JI Ecol. Complex. PD MAR PY 2010 VL 7 IS 1 BP 69 EP 75 DI 10.1016/j.ecocom.2009.05.012 PG 7 WC Ecology SC Environmental Sciences & Ecology GA 574YL UT WOS:000276030000009 ER PT J AU Del Grosso, S Parton, W AF Del Grosso, Stephen Parton, William TI Global potential net primary production predicted from vegetation class, precipitation, and temperature: reply SO ECOLOGY LA English DT Editorial Material C1 [Del Grosso, Stephen] Agr Res Serv, USDA, Ft Collins, CO 80526 USA. [Del Grosso, Stephen; Parton, William] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Del Grosso, S (reprint author), Agr Res Serv, USDA, Ft Collins, CO 80526 USA. EM delgro@nre.colostate.edu NR 4 TC 1 Z9 1 U1 4 U2 21 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD MAR PY 2010 VL 91 IS 3 BP 923 EP 925 DI 10.1890/09-0741.1 PG 3 WC Ecology SC Environmental Sciences & Ecology GA 582XZ UT WOS:000276635500029 ER PT J AU Merten, EC Hemstad, NA Eggert, SL Johnson, LB Kolka, RK Newman, RM Vondracek, B AF Merten, E. C. Hemstad, N. A. Eggert, S. L. Johnson, L. B. Kolka, R. K. Newman, R. M. Vondracek, B. TI Relations between fish abundances, summer temperatures, and forest harvest in a northern Minnesota stream system from 1997 to 2007 SO ECOLOGY OF FRESHWATER FISH LA English DT Article DE Forest harvest; temperature; stream warming; basin scale ID SOUTHERN APPALACHIAN STREAMS; MUDMINNOW UMBRA-LIMI; HEADWATER STREAMS; CLIMATE-CHANGE; WOODY DEBRIS; COMMUNITY STRUCTURE; DECIDUOUS FOREST; ORGANIC-MATTER; PRAIRIE STREAM; HABITAT AB Short-term effects of forest harvest on fish habitat have been well documented, including sediment inputs, leaf litter reductions, and stream warming. However, few studies have considered changes in local climate when examining postlogging changes in fish communities. To address this need, we examined fish abundances between 1997 and 2007 in a basin in a northern hardwood forest. Streams in the basin were subjected to experimental riparian forest harvest in fall 1997. We noted a significant decrease for fish index of biotic integrity and abundance of Salvelinus fontinalis and Phoxinus eos over the study period. However, for P. eos and Culaea inconstans, the temporal patterns in abundances were related more to summer air temperatures than to fine sediment or spring precipitation when examined using multiple regressions. Univariate regressions suggested that summer air temperatures influenced temporal patterns in fish communities more than fine sediment or spring precipitation. C1 [Merten, E. C.; Newman, R. M.] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA. [Merten, E. C.] Univ Minnesota, Water Resources Sci Grad Program, St Paul, MN 55108 USA. [Hemstad, N. A.] Inver Hills Community Coll, Dept Biol, Inver Grove Hts, MN USA. [Eggert, S. L.; Kolka, R. K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MI USA. [Johnson, L. B.] Univ Minnesota, Nat Resources Res Inst, Duluth, MN 55811 USA. [Vondracek, B.] Univ Minnesota, USGS, Minnesota Cooperat Fish & Wildlife Res Unit, St Paul, MN 55108 USA. RP Merten, EC (reprint author), Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, 1980 Folwell Ave, St Paul, MN 55108 USA. EM mert0042@umn.edu FU Minnesota Department of Natural Resources Section of Fisheries; Minnesota Forest Resources Council; National Council for Air and Stream Improvement; U.S. Forest Service, Minnesota Trout Unlimited; Minnesota Environment and Natural Resources Trust Fund FX This work was funded by the Minnesota Department of Natural Resources Section of Fisheries, the Minnesota Forest Resources Council, the National Council for Air and Stream Improvement, the U.S. Forest Service, Minnesota Trout Unlimited, and the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative Citizen Commission on Minnesota Resources. Charlie Blinn and Brian Palik selected the study sites and supervised the manipulations. John Hansen and Jim Marshall of UPM-Kymenne Corporation Blandin provided access to the study sites. Forest harvest was completed by Rieger Logging. Field assistance was provided by Andy Arola, Brenda Asmus, Jason Bronk, Rebecca Bronk, Ryan Carlson, Bill Coates, Jacquelyn Conner, MaryKay Fox, Jo Fritz, Sarah Harnden, Marty Melchior, Steffen Merten, Mateya Miltich, Brittany Mitchell, Erik Mundahl, Elliot Nitzkowski, Ian Phelps, Lisa Pugh, Jeff Rice, David Schroeder, Jeremy Steil, Kevin Stroom, Dustin Wilman, and Jason Zwonitzer. The Statistical Consulting Service at the University of Minnesota provided R code for analysis. Comments from Jacques Finlay, Heinz Stefan, and four anonymous reviewers improved the quality of the manuscript. NR 72 TC 5 Z9 6 U1 0 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-6691 EI 1600-0633 J9 ECOL FRESHW FISH JI Ecol. Freshw. Fish PD MAR PY 2010 VL 19 IS 1 BP 63 EP 73 DI 10.1111/j.1600-0633.2009.00389.x PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 558AT UT WOS:000274713100007 ER PT J AU Howell, PJ Dunham, JB Sankovich, PM AF Howell, P. J. Dunham, J. B. Sankovich, P. M. TI Relationships between water temperatures and upstream migration, cold water refuge use, and spawning of adult bull trout from the Lostine River, Oregon, USA SO ECOLOGY OF FRESHWATER FISH LA English DT Article DE Bull trout; Temperature; Thermal refuges; Water quality ID SALVELINUS-CONFLUENTUS; NORTHEASTERN OREGON; HABITAT USE; STREAMS; CHARR; MONTANA; FISHES; SYSTEM; GROWTH; IDAHO AB Understanding thermal habitat use by migratory fish has been limited by difficulties in matching fish locations with water temperatures. To describe spatial and temporal patterns of thermal habitat use by migratory adult bull trout, Salvelinus confluentus, that spawn in the Lostine River, Oregon, we employed a combination of archival temperature tags, radio tags, and thermographs. We also compared temperatures of the tagged fish to ambient water temperatures to determine if the fish were using thermal refuges. The timing and temperatures at which fish moved upstream from overwintering areas to spawning locations varied considerably among individuals. The annual maximum 7-day average daily maximum (7DADM) temperatures of tagged fish were 16-18 degrees C and potentially as high as 21 degrees C. Maximum 7DADM ambient water temperatures within the range of tagged fish during summer were 18-25 degrees C. However, there was no evidence of the tagged fish using localized cold water refuges. Tagged fish appeared to spawn at 7DADM temperatures of 7-14 degrees C. Maximum 7DADM temperatures of tagged fish and ambient temperatures at the onset of the spawning period in late August were 11-18 degrees C. Water temperatures in most of the upper Lostine River used for spawning and rearing appear to be largely natural since there has been little development, whereas downstream reaches used by migratory bull trout are heavily diverted for irrigation. Although the population effects of these temperatures are unknown, summer temperatures and the higher temperatures observed for spawning fish appear to be at or above the upper range of suitability reported for the species. C1 [Howell, P. J.] US Forest Serv, USDA, Forestry & Range Sci Lab, La Grande, OR 97850 USA. [Dunham, J. B.] Forest & Rangeland Ecosyst Sci Ctr, US Geol Survey, Corvallis, OR USA. [Sankovich, P. M.] US Fish & Wildlife Serv, Columbia River Fisheries Program Off, La Grande Field Off, La Grande, OR USA. RP Howell, PJ (reprint author), US Forest Serv, USDA, Forestry & Range Sci Lab, 1401 Gekeler Lane, La Grande, OR 97850 USA. EM phowell@fs.fed.us FU Bonneville Power Administration; USFS FX Support for this research was provided by the Bonneville Power Administration and the USFS. John Brunzell, Ian Tattam, Ari Martinez, Ian Wilson, Tim Caswell, and Steve Jacobs (ODFW) assisted with tagging and collection of field data. Gretchen Sausen (USFWS) secured initial funding and assisted with telemetry. Fisheries staff of the Nez Perce tribe provided use of their trapping facilities and trapping data. Gwynne Chandler (USFS) completed initial data summaries. Tim Whitesel (USFWS), Bill Knox (ODFW), Shelley Spalding (USFWS), and Sally Sauter (USGS) provided helpful comments on previous drafts. Use of trade or firm names in this paper are for reader information only and does not represent endorsement of any product or service by the US Government. NR 37 TC 12 Z9 12 U1 3 U2 34 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0906-6691 J9 ECOL FRESHW FISH JI Ecol. Freshw. Fish PD MAR PY 2010 VL 19 IS 1 BP 96 EP 106 DI 10.1111/j.1600-0633.2009.00393.x PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 558AT UT WOS:000274713100010 ER PT J AU Mickle, PF Schaefer, JF Adams, SB Kreiser, BR AF Mickle, P. F. Schaefer, J. F. Adams, S. B. Kreiser, B. R. TI Habitat use of age 0 Alabama shad in the Pascagoula River drainage, USA SO ECOLOGY OF FRESHWATER FISH LA English DT Article DE Alabama shad; Alosa; anadromous; habitat use ID MIGRATION; CLUPEIDAE; FISHES; WORLD AB Alabama shad (Alosa alabamae) is an anadromous species that spawns in Gulf of Mexico drainages and is a NOAA Fisheries Species of Concern. Habitat degradation and barriers to migration are considered contributing factors to range contraction that has left just the Pascagoula River drainage population in Mississippi. We studied juvenile life history and autecology in three rivers within the drainage. We collected fish, habitat and physicochemical data in three habitat types (sandbar, open channel and bank) from June to October 2004-2006. Sandbar habitat was favoured by smaller individuals early in the year. Catch per unit effort (CPUE) decreased through the summer as larger fish began occupying bank and open channel habitat. The most parsimonious model of abundance included year and river variables, while patterns of presence and absence were best explained by river, habitat type and physiochemical variables. While all three rivers in the drainage contained Alabama shad, fish were less abundant and had lower condition values in the Chickasawhay River. Earlier work suggested the Alabama shad may gradually move downstream towards the Gulf of Mexico in their first year. However, we found no evidence of this and captured large fish high in the drainage late in the year. C1 [Mickle, P. F.; Schaefer, J. F.; Kreiser, B. R.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. [Adams, S. B.] US Forest Serv, USDA, So Res Stn, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. RP Mickle, PF (reprint author), Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. EM Paul.mickle@usm.edu RI Adams, Susan/C-3559-2008 FU NOAA [0-2003-SER1]; USFS Southern Research Station [SRS 03-CA-11330127-262]; Mississippi Department of Wildlife, Fisheries, and Parks [SWG 04]; American Sportfishing Association FishAmerica Foundation [FAF-4078R]; USM FX We thank A. Commens, G. McWhirter and C. Harwell [US Forest Service (USFS)] and B. Bowen, J. Spaeth, B. Zuber, J. Bishop, S. Jackson [University of Southern Mississippi (USM)] for field assistance. We thank the USM Department of Biological Sciences and the USFS for providing vehicles and boats. Funding was provided by NOAA Fisheries (0-2003-SER1), the USFS Southern Research Station (SRS 03-CA-11330127-262), the Mississippi Department of Wildlife, Fisheries, and Parks (SWG 04), the American Sportfishing Association FishAmerica Foundation (FAF-4078R), and USM. NR 37 TC 5 Z9 5 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-6691 EI 1600-0633 J9 ECOL FRESHW FISH JI Ecol. Freshw. Fish PD MAR PY 2010 VL 19 IS 1 BP 107 EP 115 DI 10.1111/j.1600-0633.2009.00395.x PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 558AT UT WOS:000274713100011 ER PT J AU Richardson, BA Richardson, MJ Gonzalez, G Shiels, AB Srivastava, DS AF Richardson, Barbara A. Richardson, Michael J. Gonzalez, Grizelle Shiels, Aaron B. Srivastava, Diane S. TI A Canopy Trimming Experiment in Puerto Rico: The Response of Litter Invertebrate Communities to Canopy Loss and Debris Deposition in a Tropical Forest Subject to Hurricanes SO ECOSYSTEMS LA English DT Article DE canopy gaps; community composition; forest manipulation; fungi; litterbags; relative abundance ID LUQUILLO EXPERIMENTAL FOREST; MONTANE RAIN-FORESTS; ARTHROPOD ABUNDANCE; FEEDING PREFERENCES; SUBTROPICAL WET; DECOMPOSITION; HUGO; COLLEMBOLA; AVAILABILITY; ECOSYSTEM AB Hurricanes cause canopy removal and deposition of pulses of litter to the forest floor. A Canopy Trimming Experiment (CTE) was designed to decouple these two factors, and to investigate the separate abiotic and biotic consequences of hurricane-type damage and monitor recovery processes. As part of this experiment, effects on forest floor invertebrate communities were studied using litterbags. Canopy opening resulted in increased throughfall, soil moisture and light levels, but decreased litter moisture. Of these, only throughfall and soil moisture had returned to control levels 9 months after trimming. Canopy opening was the major determinant of adverse changes in forest floor invertebrate litter communities, by reducing diversity and biomass, irrespective of debris deposition, which played a secondary role. Plots subjected to the most disturbance, with canopy removed and debris added, had the lowest diversity and biomass. These two parameters were higher than control levels when debris was added to plots with an intact canopy, demonstrating that increased nutrient potential or habitat complexity can have a beneficial effect, but only if the abiotic conditions are suitable. Animal abundance remained similar over all treatments, because individual taxa responded differently to canopy trimming. Mites, Collembola, and Psocoptera, all microbiovores feeding mainly on fungal hyphae and spores, responded positively, with higher abundance in trimmed plots, whereas all other taxa, particularly predators and larger detritivores, declined in relative abundance. Litterbag mesh size and litter type had only minor effects on communities, and canopy trimming and debris deposition explained most variation between sites. Effects of trimming on diversity, biomass, and abundance of some invertebrate taxa were still seen when observations finished and canopy closure was complete at 19 months. This suggests that disturbance has a long-lasting effect on litter communities and may, therefore, delay detrital processing, depending on the severity of canopy damage and rate of regrowth. C1 [Richardson, Barbara A.; Richardson, Michael J.] Univ Puerto Rico, Inst Trop Ecosyst Studies, Luquillo LTER, San Juan, PR 00936 USA. [Gonzalez, Grizelle] US Forest Serv, Int Inst Trop Forestry, USDA, San Juan, PR 00926 USA. [Shiels, Aaron B.] Univ Hawaii Manoa, Honolulu, HI 96822 USA. [Srivastava, Diane S.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada. [Srivastava, Diane S.] Univ British Columbia, Biodivers Res Ctr, Vancouver, BC V6T 1Z4, Canada. RP Richardson, BA (reprint author), 165 Braid Rd, Edinburgh EH10 6JE, Midlothian, Scotland. EM barichardson@clara.co.uk OI Gonzalez, Grizelle /0000-0003-3007-5540 FU National Science Foundation to the Institute for Tropical Ecosystem Studies (ITES) [DEB-0218039, DEB-0620910]; University of Puerto Rico (UPR); International Institute for Tropical Forestry USDA Forest Service; Luquillo Long-Term Ecological Research Program in Puerto Rico FX This research was supported by Grants DEB-0218039 and DEB-0620910 from the National Science Foundation to the Institute for Tropical Ecosystem Studies (ITES), University of Puerto Rico (UPR), and the International Institute for Tropical Forestry USDA Forest Service. These grants support the Luquillo Long-Term Ecological Research Program in Puerto Rico. We thank Elizabeth Reese, Nicholas Brokaw, Jess Zimmerman and Ligia Lebron for project management; Maria M Rivera, Elizabeth Reese, Samuel Moya, Veronica Cruz, Carlos Estrada, Carlos Torrens, Elias Iglesias, Humberto Robles, Christina M Murphy, John Bithorn, Maria Aponte, Samuel Matta, and Ligia Lebron for assistance with many aspects of the fieldwork; Dan L Smith for analysis of canopy photographs; many student volunteers who helped with the physically demanding task of canopy collection and redistribution; D Jean Lodge (USDA Forest Service, NRS) and J Zimmerman (UPR-ITES) for environmental data, discussion and many helpful comments on the manuscript; and two anonymous reviewers for their constructive comments. NR 51 TC 26 Z9 26 U1 4 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2010 VL 13 IS 2 BP 286 EP 301 DI 10.1007/s10021-010-9317-6 PG 16 WC Ecology SC Environmental Sciences & Ecology GA 571WD UT WOS:000275785700009 ER PT J AU Sponseller, BA Strait, E Jergens, A Trujillo, J Harmon, K Koster, L Jenkins-Moore, M Killian, M Swenson, S Bender, H Waller, K Miles, K Pearce, T Yoon, KJ Nara, P AF Sponseller, Brett A. Strait, Erin Jergens, Albert Trujillo, Jessie Harmon, Karen Koster, Leo Jenkins-Moore, Melinda Killian, Mary Swenson, Sabrina Bender, Holly Waller, Ken Miles, Kristina Pearce, Tracy Yoon, Kyoung-Jin Nara, Peter TI Influenza A Pandemic (H1N1) 2009 Virus Infection in Domestic Cat SO EMERGING INFECTIOUS DISEASES LA English DT Article ID UNITED-STATES; SWINE; TRANSMISSION; PIGS; H3N2; H5N1; DISEASE; DOGS AB Influenza A pandemic (H1N1) 2009 virus continues to rapidly spread worldwide. In 2009, pandemic (H1N1) 2009 infection in a domestic cat from Iowa was diagnosed by a novel PCR assay that distinguishes between Eurasian and North American pandemic (H1N1) 2009 virus matrix genes. Human-to-cat transmission is presumed. C1 [Sponseller, Brett A.] Iowa State Univ, Coll Vet Med 2134, Ames, IA 50011 USA. [Koster, Leo; Jenkins-Moore, Melinda; Killian, Mary; Swenson, Sabrina] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Sponseller, Brett A.] Iowa State Univ, Coll Vet Med, Dept Vet Clin Sci, Ames, IA 50011 USA. [Sponseller, Brett A.] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. RP Sponseller, BA (reprint author), Iowa State Univ, Coll Vet Med 2134, 1600 S 16th St, Ames, IA 50011 USA. EM baspon@iastate.edu FU Iowa State University Office of the Vice President for Research and Economic Development; Iowa Healthy Livestock Initiative Research Grant; Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine FX This study was supported by the Iowa State University Office of the Vice President for Research and Economic Development, the Iowa Healthy Livestock Initiative Research Grant, and the Center for Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine. NR 13 TC 56 Z9 58 U1 3 U2 7 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAR PY 2010 VL 16 IS 3 BP 534 EP 537 DI 10.3201/eid1603.091737 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 566WR UT WOS:000275404700031 PM 20202440 ER PT J AU El-Nashaar, HM Banowetz, GM Peterson, CJ Griffith, SM AF El-Nashaar, H. M. Banowetz, G. M. Peterson, C. J. Griffith, S. M. TI Genetic Variability of Elemental Concentration in Winter Wheat Straw SO ENERGY & FUELS LA English DT Article ID PACIFIC-NORTHWEST; BIOMASS FUELS; COMBUSTION; PYROLYSIS; QUALITY; ASH; SWITCHGRASS; ENVIRONMENT; GRASSES AB Winter wheat (Triticum aestivum L.) occupied 79% of the Pacific Northwest (PNW) total acreage devoted to wheat production in 2007 and represented the foundation of many agricultural enterprises in this region. Straw produced as a co-product of the grain crop has potential as a feedstock for bioenergy production, but certain elements, including silicon (Si) and alkalis, that are characteristic of cereal straws restrict their use in thermochemical technologies. Little is known about genotypic differences in the elemental content among PNW winter wheat cultivars or the amount of macro- and micronutrients removed by straw harvest. In this study, the concentrations of Si and 19 other elements in straw harvested from 20 wheat cultivars grown at three locations in Oregon were quantified. There was significant genetic variability in the concentration of certain elements among these cultivars, and location affected the element accumulation. The Si content varied 3-fold, ranging from 10 604 to 30 903 mg kg(-1). On average, the harvest of wheat straw removed 6.42 g of nitrogen (N), 0.4 g of phosphorus (P), and 14 g of potassium (K) kg(-1). The harvest of wheat tissues below the flag leaves removed an additional 6.43, 0.23, and 17.8 g of N, P, and K kg(-1), respectively. While the yield and desirable end-use traits will remain the goal of breeding programs, these results suggest that selections for lower concentrations of undesirable elements, such as Si, K, and chlorine (Cl), could enhance the utility of straw for thermochemical conversion to bioenergy and other co-products. C1 [El-Nashaar, H. M.; Banowetz, G. M.; Griffith, S. M.] ARS, USDA, Corvallis, OR 97331 USA. [Peterson, C. J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. RP Banowetz, GM (reprint author), ARS, USDA, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM banowetg@onid.orst.edu NR 23 TC 5 Z9 5 U1 0 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD MAR PY 2010 VL 24 BP 2020 EP 2027 DI 10.1021/ef901181h PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 581YY UT WOS:000276563100071 ER PT J AU Knothe, G AF Knothe, Gerhard TI Biodiesel Derived from a Model Oil Enriched in Palmitoleic Acid, Macadamia Nut Oil SO ENERGY & FUELS LA English DT Article ID FREE FATTY-ACIDS; METHYL-ESTERS; FUEL PROPERTIES; SEED OIL; PETRODIESEL; COMPONENTS; STABILITY; MUTANT AB Numerous vegetable oils, animal fats, or other feedstocks have been investigated to obtain biodiesel, defined as the monoalkyl esters of vegetable oils and animal fats. While biodiesel is competitive with petrodiesel, technical problems facing biodiesel include cold flow and oxidative stability. Most biodiesel fuels largely contain five fatty acids, palmitic, stearic, oleic, linoleic, and linolenic fatty acids, in varying amounts in their fatty acid profiles. Dependent upon the major fatty acids present, biodiesel from different feedstocks faces these technical problems with varying severity. As previous work has indicated, enrichment of other fatty acids, such as decanoic or palmitoleic acid, in the fatty acid profile may be advantageous to address the technical issues facing biodiesel. In this work, an oil moderately enriched in palmitoleic acid (approximately 16-20%), macadamia nut oil, was selected for producing the corresponding biodiesel fuel and investigating its fuel properties. Methyl esters of macadamia nut oil were prepared by conventional transesterification with sodium methoxide. Fuel properties, such as cetane number, kinematic viscosity, oxidative stability, cold flow, as well as lubricity, are discussed in light of biodiesel standards. The approximately 15% content of saturated fatty esters in macadamia nut oil affects cold flow. The I H nuclear magnetic resonance (NMR) spectrum of macadamia methyl esters is also reported. C1 ARS, USDA, NCAUR, Peoria, IL 61604 USA. RP Knothe, G (reprint author), ARS, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov NR 44 TC 28 Z9 30 U1 4 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD MAR PY 2010 VL 24 BP 2098 EP 2103 DI 10.1021/ef9013295 PG 6 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 581YY UT WOS:000276563100081 ER PT J AU Xu, JL Cheng, JJ Sharma-Shivappa, RR Burns, JC AF Xu, Jiele Cheng, Jay J. Sharma-Shivappa, Ratna R. Burns, Joseph C. TI Sodium Hydroxide Pretreatment of Switchgrass for Ethanol Production SO ENERGY & FUELS LA English DT Article ID DILUTE-ACID PRETREATMENT; ENZYMATIC SACCHARIFICATION; LIME PRETREATMENT; CORN STOVER; LIGNOCELLULOSIC MATERIALS; AGRICULTURAL RESIDUES; HYDROLYSIS; ALKALI; BIOETHANOL; BIOMASS AB Lignocellulose-to-ethanol conversion is a promising technology to supplement corn-based ethanol production. However, the recalcitrant structure of lignocellulosic material is a major obstacle to the efficient conversion. To improve the enzymatic digestibility of switchgrass for the fermentable sugar production in hydrolysis, sodium hydroxide pretreatment of the biomass feedstock was investigated. At 121, 50, and 21 degrees C, raw switchgrass biomass at a solid/liquid ratio of 0.1 g/mL was pretreated, respectively, for 0.25-1, 1-48, and 1-96 h at different NaOH concentrations (0.5, 1.0, and 2.0%, w/v). Pretreatments were evaluated based on the yields of lignocellulose-derived sugars in the subsequent enzymatic hydrolysis. At the best pretreatment conditions (50 degrees C, 12 h, and 1.0% NaOH), the yield of' total reducing sugars was 453.4 mg/g raw biomass, which was 3.78 times that of untreated biomass, and the glucan and xylan conversions reached 74.4 and 62.8%, respectively. Lignin reduction was closely related to the degree of pretreatment. The maximum lignin reductions were 85.8% at 121 degrees C, 77.8% at 50 degrees C, and 62.9% at 21 degrees C, all of which were obtained at the combinations of the longest residence times and the greatest NaOH concentration. Cellulase and cellobiase loadings of 15 FPU/g dry biomass and 20 CBU/g dry biomass were sufficient to maximize sugar production. C1 [Xu, Jiele; Cheng, Jay J.; Sharma-Shivappa, Ratna R.] N Carolina State Univ, Dept Biol & Agr Engn, Raleigh, NC 27695 USA. [Burns, Joseph C.] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. [Burns, Joseph C.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Cheng, JJ (reprint author), N Carolina State Univ, Dept Biol & Agr Engn, Campus Box 7625, Raleigh, NC 27695 USA. EM jay_cheng@ncsu.edu FU North Carolina Agricultural Research Service (NCARS); North Carolina Agricultural Foundation (NCAF) FX The authors would like to acknowledge the financial support of this research from North Carolina Agricultural Research Service (NCARS) and North Carolina Agricultural Foundation (NCAF). NR 27 TC 96 Z9 100 U1 1 U2 49 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD MAR PY 2010 VL 24 BP 2113 EP 2119 DI 10.1021/ef9014718 PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 581YY UT WOS:000276563100084 ER PT J AU Li, SJ Crane, YM Sun, L Schemerhorn, BJ Oseto, CY Ferris, VR AF Li, Shujuan Crane, Y. M. Sun, L. Schemerhorn, B. J. Oseto, C. Y. Ferris, V. R. TI CHARACTERIZATION OF MICROSATELLITE LOCI IN SMICRONYX SORDIDUS, THE GRAY SUNFLOWER SEED WEEVIL (COLEOPTERA: CURCULIONIDAE) SO ENTOMOLOGICAL NEWS LA English DT Article ID EASTERN NORTH-AMERICA; MARKERS; ORIGIN; DNA C1 [Crane, Y. M.; Schemerhorn, B. J.] Purdue Univ, Dept Entomol, USDA ARS, W Lafayette, IN 47907 USA. RP Li, SJ (reprint author), Univ Arizona, Maricopa Agr Ctr, 37860 W Smith Enke Rd, Maricopa, AZ 85239 USA. EM shujuan-li2007@gmail.com; ycrane@purdue.edu; systentomol@gmail.com; bschemer@purdue.edu; osetoc@purdue.edu; vferris@purdue.edu OI Schemerhorn, Brandon/0000-0003-0247-478X FU Purdue Research Foundation; Department of Entomology at Purdue University FX We thank Drs. Jeffrey Stuart and Bob Waltz for critical reading of the manuscript. This work was supported by the Purdue Research Foundation and the Department of Entomology at Purdue University. NR 15 TC 0 Z9 0 U1 0 U2 1 PU AMER ENTOMOL SOC PI PHILADELPHIA PA 1900 BENJ FRANKLIN PARKWAY, PHILADELPHIA, PA 19103-1195 USA SN 0013-872X J9 ENTOMOL NEWS JI Entomol. News PD MAR-APR PY 2010 VL 121 IS 2 BP 195 EP 199 PG 5 WC Entomology SC Entomology GA 787JB UT WOS:000292371300012 ER PT J AU Favret, C Miller, GL AF Favret, Colin Miller, Gary L. TI C.V. RILEY'S LOST APHIDS: SIPHONOPHORA FRAGARIAE VAR. IMMACULATA AND APHIS RAPAE VAR. LAEVIGATA (HEMIPTERA: APHIDIDAE) SO ENTOMOLOGICAL NEWS LA English DT Article DE nomen oblitum; nomen protectum; Macrosiphum euphorbiae; Myzus persicae; potato aphid; green peach aphid; new synonymy ID MACROSIPHUM-EUPHORBIA; LIST AB The syntypes of Siphonophora fragariae var. immaculata Riley were rediscovered in the aphid collection of the United States of America National Museum of Natural History. The name (S. fragariae immaculata) previously being largely lost and forgotten, we here establish it as the senior synonym of Macrosiphum euphorbiae (Thomas). Reversal of precedence can be validated, however, and we establish S. fragariae immaculata as a nomen oblitum and M. euphorbiae a nomen protection with respect to each other. In uncovering the original description of the Riley species, we also found a description of Aphis rapae var. laevigata Riley. We have not found A. rapae laevigata anywhere else in the literature nor have we found any type specimens. Based on Riley's succinct description and host plant identity, we consider it a nomen dubium synonym of Myzus persicae (Sulzer). Because the short articles publishing the two aphid names have been missing from taxonomists' libraries, they are transcribed in their entirety. C1 [Favret, Colin] AphidNet LLC, Gaithersburg, MD 20879 USA. [Miller, Gary L.] ARS, USDA, Systemat Entomol Lab, Beltsville, MD 20705 USA. RP Favret, C (reprint author), AphidNet LLC, 18901 Tributary Ln, Gaithersburg, MD 20879 USA. EM ColinFavret@AphidNet.org; Gary.Miller@ars.usda.gov RI Favret, Colin/F-7726-2012 OI Favret, Colin/0000-0001-6243-3184 NR 42 TC 0 Z9 0 U1 0 U2 2 PU AMER ENTOMOL SOC PI PHILADELPHIA PA 1900 BENJ FRANKLIN PARKWAY, PHILADELPHIA, PA 19103-1195 USA SN 0013-872X J9 ENTOMOL NEWS JI Entomol. News PD MAR-APR PY 2010 VL 121 IS 2 BP 200 EP 205 PG 6 WC Entomology SC Entomology GA 787JB UT WOS:000292371300013 ER PT J AU McMichael, BL Lascano, RJ AF McMichael, B. L. Lascano, Robert J. TI Evaluation of hydraulic lift in cotton (Gossypium hirsutum L.) germplasm SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Cotton; Roots; Water uptake; ET; Water content ID WATER; SOIL; ROOTS; SYSTEM; PLANTS; MAIZE AB Hydraulic lift (HL) in plants is defined as the redistribution of water from wetter to drier soil through the plant roots in response to soil water potential gradients. Water is released from the roots into the dry soil when transpiration is low (night) and reabsorbed by the plant when higher transpiration rates are resumed (daylight). It was hypothesized that since HL is not of sufficient magnitude to meet total transpirational demands, there may be sufficient water transferred to maintain viable roots in the surface soil in anticipation of root water uptake for plant development from rain events before it is lost to water evaporation and/or runoff. Cotton (Gossypium hirsutum L.) plants of diverse genetic backgrounds were grown in a split-root container with roots of one plant divided between the two adjacent soil volumes. The soil in one container was allowed to dry while the adjacent soil container remained wet for the duration of the tests. Cotton transpiration was lowered by covering the plant with black cloth for several hours while changes in soil water content were measured in both containers using calibrated soil water sensors. Results showed that water was transferred from the wet to the dry soil through the root system at low transpiration rates. There was also an indication of genetic diversity in the magnitude of HL that may be due to differences in root resistance to water flow since total root length for a particular genotype was either smaller in the dry soil or similar to total root length in the wet soil. The amount of water transferred was small, but when integrated over a soil depth represented an amount ranging from 11% to 32% of corresponding daily evapotranspiration rates of 2-6 mm d(-1), respectively, which could presumably maintain root viability for additional root water uptake when made available. These results are important in dryland cotton production where additional transpiration represents increased lint yield and plant breeders may consider this trait in their selection of cotton germplasm with drought resistance. Published by Elsevier B.V. C1 [McMichael, B. L.; Lascano, Robert J.] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Lascano, RJ (reprint author), ARS, USDA, Cropping Syst Res Lab, 3810 4th St, Lubbock, TX 79415 USA. EM Robert.lascano@ars.usda.gov FU Cotton Incorporated [05-702] FX The authors wish to acknowledge Cotton Incorporated (Project No. 05-702) for their continued support of the project. NR 19 TC 7 Z9 7 U1 1 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD MAR PY 2010 VL 68 IS 1 BP 26 EP 30 DI 10.1016/j.envexpbot.2009.10.002 PG 5 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA 556PN UT WOS:000274603600003 ER PT J AU Preisler, HK Zhong, SY Esperanza, A Brown, TJ Bytnerowicz, A Tarnay, L AF Preisler, Haiganoush K. Zhong, Shiyuan (Sharon) Esperanza, Annie Brown, Timothy J. Bytnerowicz, Andrzej Tarnay, Leland TI Estimating contribution of wildland fires to ambient ozone levels in National Parks in the Sierra Nevada, California SO ENVIRONMENTAL POLLUTION LA English DT Article DE BlueSky; California air-quality standard; Next-day ozone forecasts; Air pollution; Fire smoke; Regression models; Spline functions; Time series AB Data from four continuous ozone and weather monitoring sites operated by the National Park Service in Sierra Nevada, California, are used to develop an ozone forecasting model and to estimate the contribution of wildland fires on ambient ozone levels. The analyses of weather and ozone data pointed to the transport of ozone precursors from the Central Valley as an important source of pollution in these National Parks. Comparisons of forecasted and observed values demonstrated that accurate forecasts of next-day hourly ozone levels may be achieved by using a tithe series model with historic averages, expected local weather and modeled PM values as explanatory variables Results on fire smoke influence indicated occurrence of significant increases in average ozone levels with increasing fire activity The overall effect on diurnal ozone values. however, was small when compared with the amount of variability attributed to sources other than fire Published by Elsevier Ltd C1 [Preisler, Haiganoush K.] USDA Forest Serv, Pacific SW Res Stn, Albany, CA 94710 USA. [Zhong, Shiyuan (Sharon)] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. [Brown, Timothy J.] Desert Res Inst, Reno, NV 89521 USA. [Bytnerowicz, Andrzej] USDA Forest Serv, Pacific SW Res Stn, Riverside, CA 92507 USA. [Tarnay, Leland] Yosemite Natl Pk, El Portal, CA 95318 USA. RP Preisler, HK (reprint author), USDA Forest Serv, Pacific SW Res Stn, 800 Buchanan St, Albany, CA 94710 USA. RI Bytnerowicz, Andrzej/A-8017-2013 FU USDA [JFSP AFP 2005-3] FX We would like to thank Domagoj Podnar for providing BIueSky output data at the four locations and the National Park Service Air Resources Division for the ozone and meteorological data The project was supported by the USDA Forest Service Joint Fire Science Program # JFSP AFP 2005-3 (Andrzej Bytnerowicz Principal Investigator). NR 16 TC 6 Z9 6 U1 1 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 J9 ENVIRON POLLUT JI Environ. Pollut. PD MAR PY 2010 VL 158 IS 3 BP 778 EP 787 DI 10.1016/j.envpol.2009.10.007 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA 561TU UT WOS:000275002900018 PM 19914752 ER PT J AU Torkzaban, S Kim, HN Simunek, J Bradford, SA AF Torkzaban, Saeed Kim, Hyunjung N. Simunek, Jiri Bradford, Scott A. TI Hysteresis of Colloid Retention and Release in Saturated Porous Media During Transients in Solution Chemistry SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SECONDARY ENERGY MINIMUM; MICROMETER-SCALE PARTICLES; IONIC-STRENGTH; CHEMICAL CONDITIONS; PATCHY SURFACES; TRANSPORT; DEPOSITION; ADHESION; WATER; FLOW AB Saturated packed column and micromodel transport studies were conducted to gain insight on mechanisms of colloid retention and release under unfavorable attachment conditions. The initial deposition of colloids in porous media was found to be a strongly coupled process that depended on solution chemistry and pore space geometry. During steady state chemical conditions, colloid deposition was not a readily reversible process, and micromodel photos indicated that colloids were immobilized in the presence of fluid drag. Upon stepwise reduction in eluting solution ionic strength (IS), a sharp release of colloids occurred in each step which indicates that colloid retention depends on a balance of applied (hydrodynamic) and resisting (adhesive) torques which varied with pore space geometry, surface roughness, and interaction energy. When the eluting fluid IS was reduced to deionized water, the final retention locations occurred near grain-grain contacts, and colloid aggregation was sometimes observed in micromodel experiments. Significant amounts of colloid retention hysteresis with IS were observed in the column experiments, and it depended on the porous medium (glass beads compared with sand), the colloid size (1.1 and 0.5 mu m), and on the initial deposition IS. These observations were attributed to weak adhesive interactions that depended on the double layer thickness (e.g., the depth of the secondary minimum and/or nanoscale heterogeneity), colloid mass transfer on the solid phase to regions where the torque and force balances were favorable for retention, the number and extent of grain-grain contacts, and surface roughness. C1 [Bradford, Scott A.] ARS, USDA, US Salin Lab, Riverside, CA USA. [Torkzaban, Saeed] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Earth Sci, Berkeley, CA 94720 USA. [Kim, Hyunjung N.] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA. [Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Bradford, SA (reprint author), ARS, USDA, US Salin Lab, Riverside, CA USA. EM Scott.Bradford@ars.usda.gov RI Simunek, Jiri/F-3196-2011; Kim, Hyunjung/F-1505-2013; Torkzaban, Saeed/G-7377-2013 OI Kim, Hyunjung/0000-0003-2115-6891; Torkzaban, Saeed/0000-0002-5146-9461 FU USDA-ARS; NRI [NRI 2006-02541] FX This research was supported by the 206 Manure and Byproduct Utilization Project of the USDA-ARS, and by a grant from NRI (NRI 2006-02541). Mention of trade names and company names in this manuscript does not imply any endorsement or preferential treatment by the USDA. NR 41 TC 45 Z9 45 U1 2 U2 33 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAR 1 PY 2010 VL 44 IS 5 BP 1662 EP 1669 DI 10.1021/es903277p PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 559QT UT WOS:000274842000025 PM 20136144 ER PT J AU An, CF Jenkins, JN Wu, JX Guo, YF McCarty, JC AF An, Chuanfu Jenkins, Johnie N. Wu, Jixiang Guo, Yufang McCarty, Jack C. TI Use of fiber and fuzz mutants to detect QTL for yield components, seed, and fiber traits of upland cotton SO EUPHYTICA LA English DT Article DE Cotton; Fiber mutants; Quantitative trait loci ID GOSSYPIUM-HIRSUTUM L.; GENETIC-ANALYSIS; LINT PERCENTAGE; QUANTITATIVE TRAITS; SUCROSE SYNTHASE; MARKER GENES; LINKAGE MAP; IDENTIFICATION; QUALITY; RESISTANCE AB This research detected QTL or molecular markers associated with yield, fiber, and seed traits within multiple fuzz and fiber loci genetic backgrounds. Two F(2) populations from crosses of MD17, a fuzzless-lintless line containing three fuzzless loci, N (1) , n (2) and a postulated n (3) , with line 181, fuzzless-linted and with FM966, a fuzzy-linted cultivar, were used. QTL explaining 68.3 (population with FM966) to 87.1% (population with 181) of the phenotypic variation for lint percentage and 62.8% (population with 181) for lint index were detected in the vicinity of BNL3482-138 on chromosome 26. Single marker regression analyses indicated STV79-108, on the long arm of chromosome 12 had significant association with lint percentage (R (2) 26.7%), lint index (R (2) 30.6%), embryo protein percentage (R (2) 15.4%) and micronaire (R (2) 20.0%). Two-locus epistatic interactions were also observed. Results from this research will facilitate further understanding the complex mechanisms of yield, fiber, and seed traits of cotton. C1 [Jenkins, Johnie N.; McCarty, Jack C.] USDA ARS, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [An, Chuanfu; Wu, Jixiang; Guo, Yufang] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA. RP Jenkins, JN (reprint author), USDA ARS, Crop Sci Res Lab, POB 5367, Mississippi State, MS 39762 USA. EM Johnie.Jenkins@ars.usda.gov RI An, Chuanfu/G-5521-2011; Guo, Yufang/G-9363-2011; Wu, Jixiang/C-4084-2009 NR 62 TC 23 Z9 25 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAR PY 2010 VL 172 IS 1 BP 21 EP 34 DI 10.1007/s10681-009-0009-2 PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 555VL UT WOS:000274544200003 ER PT J AU Chamberlin, KDC Melouk, HA Payton, ME AF Chamberlin, Kelly D. Chenault Melouk, Hassan A. Payton, Mark E. TI Evaluation of the US peanut mini core collection using a molecular marker for resistance to Sclerotinia minor Jagger SO EUPHYTICA LA English DT Article DE Mini-core collection; Peanut; Sclerotinia blight; Molecular marker; Resistance ID ARACHIS-HYPOGAEA L.; GERMPLASM COLLECTION; PHASEOLUS-VULGARIS; REGISTRATION; VARIETIES; SELECTION AB Cultivated peanut, the second most economically important legume crop throughout the United States and the third most important oilseed in the world, is consistently threatened by various diseases and pests. Sclerotinia minor Jagger (S. minor), the causal agent of Sclerotinia blight, is a major threat to peanut production in the Southwestern U.S., Virginia, and North Carolina and can reduce yield by up to 50% in severely infested fields. Although host plant resistance would provide the most effective solution to managing Sclerotinia blight, limited sources of resistance to the disease are available for use in breeding programs. Peanut germplasm collections are available for exploration and identification of new sources of resistance, but traditionally the process is lengthy, requiring years of field testing before those potential sources can be identified. Molecular markers associated with phenotypic traits can speed up the screening of germplasm accessions, but until recently none were available for Sclerotinia blight resistance in peanut. This study objective of this study was to characterize the US peanut mini-core collection with regards to a recently discovered molecular marker associated with Sclerotinia blight resistance. Ninety-six accessions from the collection were available and genotyped using the SSR marker and 39 total accessions from spanish, valencia, runner market types were identified as new potential sources of resistance and targeted for further evaluation in field tests for Sclerotinia blight resistance. C1 [Chamberlin, Kelly D. Chenault; Melouk, Hassan A.] USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Payton, Mark E.] Oklahoma State Univ, Dept Stat, Stillwater, OK 74078 USA. RP Chamberlin, KDC (reprint author), USDA ARS, Wheat Peanut & Other Field Crops Res Unit, 1301 N Western, Stillwater, OK 74075 USA. EM kelly.chamberlin@ars.usda.gov NR 26 TC 9 Z9 12 U1 1 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAR PY 2010 VL 172 IS 1 BP 109 EP 115 DI 10.1007/s10681-009-0065-7 PG 7 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 555VL UT WOS:000274544200011 ER PT J AU Anderson, JM Bucholtz, DL Sardesai, N Santini, JB Gyulai, G Williams, CE Goodwin, SB AF Anderson, Joseph M. Bucholtz, Dennis L. Sardesai, Nagesh Santini, Judith B. Gyulai, Gabor Williams, Christie E. Goodwin, Stephen B. TI Potential new genes for resistance to Mycosphaerella graminicola identified in Triticum aestivum x Lophopyrum elongatum disomic substitution lines SO EUPHYTICA LA English DT Article DE Barley yellow dwarf virus; Cereal yellow dwarf virus; Resistance; Septoria tritici blotch; Wheatgrass substitution lines; Wheat breeding ID BARLEY YELLOW-DWARF; HEAD BLIGHT RESISTANCE; HESSIAN FLY DIPTERA; CONFERRING RESISTANCE; ELYTRIGIA-ELONGATA; VIRUS-RESISTANCE; HEXAPLOID WHEAT; CHROMOSOMES; BLOTCH; CHROMATIN AB Lophopyrum species carry many desirable agronomic traits, including disease resistance, which can be transferred to wheat by interspecific hybridization. To identify potentially new genes for disease and insect resistance carried by individual Lophopyrum chromosomes, 19 of 21 possible wheat cultivar Chinese Spring x Lophopyrum elongatum disomic substitution lines were tested for resistance to barley yellow dwarf virus (BYDV), cereal yellow dwarf virus (CYDV), the Hessian fly Mayetiola destructor, and the fungal pathogens Blumeria graminis and Mycosphaerella graminicola (asexual stage: Septoria tritici). Low resistance to BYDV occurred in some of the disomic substitution lines, but viral titers were significantly higher than those of two Lophopyrum species tested. This suggested that genes on more than one Lophopyrum chromosome are required for complete resistance to this virus. A potentially new gene for resistance to CYDV was detected on wheatgrass chromosome 3E. All of the substitution lines were susceptible to Mayetiola destructor and one strain of B. graminis. Disomic substitution lines containing wheatgrass chromosomes 1E and 6E were significantly more resistant to M. graminicola compared to Chinese Spring. Although neither chromosome by itself conferred resistance as high as that in the wheatgrass parent, they do appear to contain potentially new genes for resistance against this pathogen that could be useful for future plant-improvement programs. C1 [Anderson, Joseph M.; Bucholtz, Dennis L.; Williams, Christie E.; Goodwin, Stephen B.] Purdue Univ, ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Sardesai, Nagesh] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Santini, Judith B.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Gyulai, Gabor] St Stephanus Univ, HAS SIU, Inst Genet & Biotechnol, H-2103 Godollo, Hungary. RP Goodwin, SB (reprint author), Purdue Univ, ARS, USDA, Crop Prod & Pest Control Res Unit, 915 W State St, W Lafayette, IN 47907 USA. EM Steve.Goodwin@ARS.USDA.gov FU Purdue University [3602-22000-014-03S]; USDA-ARS CRIS [3602-22000-012-00D, 3602-21220-013-00D, 3602-21220-008-00D]; Hungarian-American Fulbright Commission [1201 102] FX The authors wish to thank Jill Breeden, George Buechley, Brett Roberts, and Bryan Wallace for their technical contributions with the disease screening or molecular marker experiments. This work was supported by Purdue University project 3602-22000-014-03S and USDA-ARS CRIS projects 3602-22000-012-00D, 3602-21220-013-00D, and 3602-21220-008-00D. Support for G. G. was provided by the Hungarian-American Fulbright Commission, Grant No. 1201 102. NR 37 TC 4 Z9 4 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAR PY 2010 VL 172 IS 2 BP 251 EP 262 DI 10.1007/s10681-009-0061-y PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 557QT UT WOS:000274685800010 ER PT J AU Garaulet, M Lee, YC Shen, J Parnell, LD Arnett, DK Tsai, MY Lai, CQ Ordovas, JM AF Garaulet, Marta Lee, Yu-Chi Shen, Jian Parnell, Laurence D. Arnett, Donna K. Tsai, Michael Y. Lai, Chao-Qiang Ordovas, Jose M. TI Genetic variants in human CLOCK associate with total energy intake and cytokine sleep factors in overweight subjects (GOLDN population) SO EUROPEAN JOURNAL OF HUMAN GENETICS LA English DT Article DE CLOCK; circadian; obesity; energy intake; metabolic syndrome; interleukin-6 ID FOOD-FREQUENCY QUESTIONNAIRE; METABOLIC SYNDROME; TRANSCRIPTION FACTOR; CIRCADIAN-RHYTHMS; OBESITY; DISORDERS; BRAIN; INFLAMMATION; VALIDATION; EXPRESSION AB Despite the importance of total energy intake in circadian system regulation, no study has related human CLOCK gene polymorphisms and food-intake measures. The aim of this study was to analyze the associations of CLOCK single-nucleotide polymorphisms (SNPs) with food intake and to explore the specific role of the cytokine system. A total of 1100 individual participants in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study were included. Dietary intake was estimated with a validated questionnaire. Interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP1), tumor necrosis factor-alpha (TNF-alpha), IL-2 soluble receptor-alpha (IL-2sR-alpha) and adiponectin plasma concentrations were measured. Our results showed that four of five CLOCK SNPs selected were significantly associated with total energy intake (P<0.05). For SNP rs3749474, the energy intake and total fat, protein and carbohydrate intakes were significantly higher in minor allele carriers than in non-carriers. Frequency of the minor allele was greater in subjects with high energy intake than in those with low intake. Subjects with the minor allele were 1.33 times more likely to have high energy intake than non-carriers (95% CI 1.09-1.72, P=0.0350). All CLOCK SNPs were associated with plasma cytokine values, in particular with those that were highly correlated with energy intake: MCP1, IL-6 and adiponectin. Interestingly, minor allele carriers with high energy intake showed decreased cytokine values, which could be related with a lower anorectic effect and decreased sleep in these subjects. In conclusion, we show a novel association of genetic variation at CLOCK with total energy intake, which was particularly relevant for SNP rs3749474. Associations could be mediated through the alteration of cytokine levels that may influence energy intake and sleep pattern. European Journal of Human Genetics (2010) 18, 364-369; doi:10.1038/ejhg.2009.176; published online 4 November 2009 C1 [Garaulet, Marta] Univ Murcia, Fac Biol, Dept Physiol, E-30100 Murcia, Spain. [Garaulet, Marta; Lee, Yu-Chi; Shen, Jian; Parnell, Laurence D.; Lai, Chao-Qiang; Ordovas, Jose M.] Tufts Univ, Sch Med, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama, Dept Epidemiol, Birmingham, AL USA. [Tsai, Michael Y.] Univ Minnesota, Dept Expt & Clin Pharmacol, Minneapolis, MN USA. RP Garaulet, M (reprint author), Univ Murcia, Fac Biol, Dept Physiol, Campus Espinardo S-N, E-30100 Murcia, Spain. EM garaulet@um.es OI Ordovas, Jose/0000-0002-7581-5680 FU Government of Murcia [02934/PI/05]; Government of Education, Science and Research of Murcia [BIO/FFA 07/01-0004]; The Spanish Government of Science and Innovation [AGL2008-01655/ALI]; National Institutes of Health [U01 HL72524, HL-54776]; National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [DK075030]; US Department of Agriculture Research Service [53-K06-5-10, 58-1950-9-001] FX This work was supported by the Seneca Foundation from the Government of Murcia (project 02934/PI/05 to MG), the Government of Education, Science and Research of Murcia (Project BIO/FFA 07/01-0004) and by The Spanish Government of Science and Innovation (projects AGL2008-01655/ALI) and by Grants U01 HL72524 and HL-54776 from the National Institutes of Health, and DK075030 from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), and contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research Service. JMO. NR 45 TC 37 Z9 38 U1 0 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1018-4813 J9 EUR J HUM GENET JI Eur. J. Hum. Genet. PD MAR PY 2010 VL 18 IS 3 BP 364 EP 369 DI 10.1038/ejhg.2009.176 PG 6 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 556OY UT WOS:000274601800016 PM 19888304 ER PT J AU Rodriguez-Brljevich, C Kanobe, C Shanahan, JF Robertson, AE AF Rodriguez-Brljevich, C. Kanobe, C. Shanahan, J. F. Robertson, A. E. TI Seed treatments enhance photosynthesis in maize seedlings by reducing infection with Fusarium spp. and consequent disease development in maize SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Chlorophyll fluorescence; Crown rot; Photosystem II; Stalk rot ID CHLOROPHYLL FLUORESCENCE AB Maize seed that was either treated with the fungicide Cruiser Extreme 250 A (R) (fludioxonil + azoxystrobin + mefenoxam + thiamethoxam) or not treated was planted at two Iowa locations in 2007. Root, mesocotyl and crown rot severity, incidence of Fusarium spp. colonisation and chlorophyll florescence (CF) were assessed at growth stages V2, V4 and V6, and stalk rot severity at R6. At both locations, seed treatment reduced disease severity and incidence of Fusarium spp. infection at all growth stages assessed. Measurements of CF decreased significantly with increased disease severity and incidence of Fusarium spp. at V2 and V4 at both locations, indicating that seedling disease negatively affected photosynthetic performance. Mesocotyl rot severity at V4 predicted crown rot severity at V6 at both locations, as well as crown rot at V6 and stalk rot at R6 at one location. C1 [Rodriguez-Brljevich, C.; Robertson, A. E.] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA. [Kanobe, C.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Shanahan, J. F.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Robertson, AE (reprint author), Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA. EM alisonr@iastate.edu OI Shanahan, John/0000-0003-3173-4245; Robertson, Alison/0000-0003-4281-6350 FU Syngenta Crop Protection, Basel, Switzerland FX We thank Rich Pope and Mark Gleason for reviewing an earlier version of this manuscript, and Syngenta Crop Protection, Basel, Switzerland, for financial support. NR 13 TC 3 Z9 3 U1 3 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD MAR PY 2010 VL 126 IS 3 BP 343 EP 347 DI 10.1007/s10658-009-9545-9 PG 5 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 551YW UT WOS:000274251000005 ER PT J AU Caesar-TonThat, T Lenssen, AW Caesar, AJ Sainju, UM Gaskin, JF AF Caesar-TonThat, TheCan Lenssen, Andy W. Caesar, Anthony J. Sainju, Upendra M. Gaskin, John F. TI Effects of tillage on microbial populations associated to soil aggregation in dryland spring wheat system SO EUROPEAN JOURNAL OF SOIL BIOLOGY LA English DT Article DE Soil aggregation; Predominant culturable bacteria; Basidiomycetes; Dryland; Spring wheat; Tillage ID METHYL-ESTER FAME; ORGANIC-MATTER; COMMUNITY STRUCTURE; BASIDIOMYCETE FUNGUS; REDUCED TILLAGE; BACTERIAL; DIVERSITY; SEQUENCE; DYNAMICS; BIOMASS AB Tillage may influence the microbial populations involved in soil aggregation. We evaluated the effects of no till (NT) and conventional tillage (CT, tillage depth about 7 cm) continuous spring wheat system on culturable heterotrophic bacterial communities predominant in microaggregates (0.25-0.05 mm) and on soil-aggregating basidiomycete fungi in aggregate-size classes (4.75-2.00, 2.00-0.25, and 0.25-0.05 mm) at 0-20 cm depth of a Williams loam (fine-loamy, mixed, Typic Argiustolls) in dryland Montana, USA. Enzyme-linked immunosorbent assay used to quantify antigenic response to basidiomycete cell walls, was higher in NT than in CT in 4.75-2.00 mm size class in 2007 and higher in all classes and years at 0-5 cm depth, but was not different between tillage, years, and classes at 5-20 cm. The culturable bacteria from microaggregates were subjected to a soil sedimentation assay to determine their soil binding capability. The proportion of isolates which can function as soil aggregators was higher in NT than in CT at 0-5 cm but was not different at 5-20 cm. Our results provide a first insight into the beneficial effects of dryland NT compared to CT in reducing soil disturbance and residue incorporation and enriching the proportion of microorganisms responsible for aggregation, especially at the soil surface. Published by Elsevier Masson SAS. C1 [Caesar-TonThat, TheCan; Lenssen, Andy W.; Caesar, Anthony J.; Sainju, Upendra M.; Gaskin, John F.] USDA ARS, Sidney, MT 59270 USA. RP Caesar-TonThat, T (reprint author), USDA ARS, 1500 N Cent Ave, Sidney, MT 59270 USA. EM thecan.caesar@ars.usda.gov NR 61 TC 9 Z9 12 U1 1 U2 19 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 1164-5563 J9 EUR J SOIL BIOL JI Eur. J. Soil Biol. PD MAR-APR PY 2010 VL 46 IS 2 BP 119 EP 127 DI 10.1016/j.ejsobi.2009.12.004 PG 9 WC Ecology; Soil Science SC Environmental Sciences & Ecology; Agriculture GA 585PL UT WOS:000276838800009 ER PT J AU Zalapa, JE Brunet, J Guries, RP AF Zalapa, Juan E. Brunet, Johanne Guries, Raymond P. TI The extent of hybridization and its impact on the genetic diversity and population structure of an invasive tree, Ulmus pumila (Ulmaceae) SO EVOLUTIONARY APPLICATIONS LA English DT Article DE genetic diversity; hybridization; invasive species; Siberian elm; Ulmus pumila; Ulmus rubra ID EVOLUTION; INTROGRESSION; AMERICA; BIOLOGY; HYBRIDS; PLANTS; CHINA; RUBRA AB Ulmus pumila is considered an invasive tree in 41 of the United States. In this study, we examined the extent of hybridization in naturalized populations of U. pumila, its impact on genetic diversity and genetic structure and its potential role in explaining the invasion process of U. pumila. Genetic analyses indicated widespread hybridization with native Ulmus rubra in naturalized U. pumila populations. Hybridization increased the genetic diversity of U. pumila populations and affected their genetic structure. The level of genetic diversity in 'mature' accessions, many of which may represent original plantings throughout the USA, was high and similar to the diversity of East Asian accessions. Hybridization with the native red elm may play an important role in the success of Siberian elm as an invader in temperate regions of the USA. C1 [Brunet, Johanne] Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit,Dept Entomol, Madison, WI 53706 USA. [Zalapa, Juan E.; Guries, Raymond P.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Brunet, J (reprint author), Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit,Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM jbrunet@wisc.edu FU National Science Foundation [0409651]; USDA-ARS FX The authors thank the University of Wisconsin-Madison Herbarium and the many collaborators that contributed tissue for DNA analysis; Ana Bravo for her assistance with the genetic analysis; and Kurtis Cooper for helpful discussion. We gratefully acknowledge the National Science Foundation Minority Post-doctoral Fellowship to J.E. Zalapa (NSF award #0409651) and support from the USDA-ARS to J. Brunet. NR 51 TC 21 Z9 21 U1 1 U2 31 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD MAR PY 2010 VL 3 IS 2 BP 157 EP 168 DI 10.1111/j.1752-4571.2009.00106.x PG 12 WC Evolutionary Biology SC Evolutionary Biology GA 561CK UT WOS:000274952800008 PM 25567916 ER PT J AU Leger, EA Espeland, EK AF Leger, Elizabeth A. Espeland, Erin K. TI Coevolution between native and invasive plant competitors: implications for invasive species management SO EVOLUTIONARY APPLICATIONS LA English DT Article DE coevolution; competition; contemporary evolution; invasive species; management; microevolution; restoration ID HOST-PARASITOID SYSTEM; CHARACTER DISPLACEMENT; GENETIC-VARIATION; POPULATION-SIZE; INTERSPECIFIC COMPETITION; EVOLUTIONARY RESPONSES; SPATIAL HETEROGENEITY; ADAPTIVE EVOLUTION; PERMANENT PASTURE; LOCAL ADAPTATION AB Invasive species may establish in communities because they are better competitors than natives, but in order to remain community dominants, the competitive advantage of invasive species must be persistent. Native species that are not extirpated when highly invasive species are introduced are likely to compete with invaders. When population sizes and genetic diversity of native species are large enough, natives may be able to evolve traits that allow them to co-occur with invasive species. Native species may also evolve to become significant competitors with invasive species, and thus affect the fitness of invaders. Invasive species may respond in turn, creating either transient or continuing coevolution between competing species. In addition to demographic factors such as population size and growth rates, a number of factors including gene flow, genetic drift, the number of selection agents, encounter rates, and genetic diversity may affect the ability of native and invasive species to evolve competitive ability against one another. We discuss how these factors may differ between populations of native and invasive plants, and how this might affect their ability to respond to selection. Management actions that maintain genetic diversity in native species while reducing population sizes and genetic diversity in invasive species could promote the ability of natives to evolve improved competitive ability. C1 [Leger, Elizabeth A.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89512 USA. [Espeland, Erin K.] USDA ARS NPARL, Sidney, MT USA. RP Leger, EA (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Mail Stop 186,1000 Valley Rd, Reno, NV 89512 USA. EM eleger@cabnr.unr.edu NR 91 TC 30 Z9 31 U1 8 U2 82 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD MAR PY 2010 VL 3 IS 2 BP 169 EP 178 DI 10.1111/j.1752-4571.2009.00105.x PG 10 WC Evolutionary Biology SC Evolutionary Biology GA 561CK UT WOS:000274952800009 PM 25567917 ER PT J AU Dyer, AR Brown, CS Espeland, EK McKay, JK Meimberg, H Rice, KJ AF Dyer, Andrew R. Brown, Cynthia S. Espeland, Erin K. McKay, John K. Meimberg, Harald Rice, Kevin J. TI The role of adaptive trans-generational plasticity in biological invasions of plants SO EVOLUTIONARY APPLICATIONS LA English DT Article DE annual plants; competitive ability; environmental stress; inclusive fitness; maternal environmental effects; phenotypic plasticity; propagule dispersal; resource patch; seed size; spatial heterogeneity ID HORDEUM-SPONTANEUM KOCH; CYPERUS-ESCULENTUS; GENETIC-VARIATION; BROMUS-TECTORUM; CLONAL PLANT; TRANSGENERATIONAL PLASTICITY; POPULATION DIFFERENTIATION; DIFFERENT ENVIRONMENTS; PHENOTYPIC PLASTICITY; ECOLOGICAL GENETICS AB High-impact biological invasions often involve establishment and spread in disturbed, high-resource patches followed by establishment and spread in biotically or abiotically stressful areas. Evolutionary change may be required for the second phase of invasion (establishment and spread in stressful areas) to occur. When species have low genetic diversity and short selection history, within-generation phenotypic plasticity is often cited as the mechanism through which spread across multiple habitat types can occur. We show that trans-generational plasticity (TGP) can result in pre-adapted progeny that exhibit traits associated with increased fitness both in high-resource patches and in stressful conditions. In the invasive sedge, Cyperus esculentus, maternal plants growing in nutrient-poor patches can place disproportional number of propagules into nutrient-rich patches. Using the invasive annual grass, Aegilops triuncialis, we show that maternal response to soil conditions can confer greater stress tolerance in seedlings in the form of greater photosynthetic efficiency. We also show TGP for a phenological shift in a low resource environment that results in greater stress tolerance in progeny. These lines of evidence suggest that the maternal environment can have profound effects on offspring success and that TGP may play a significant role in some plant invasions. C1 [Dyer, Andrew R.] Univ S Carolina, Dept Biol & Geol, Aiken, SC USA. [Brown, Cynthia S.; McKay, John K.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Espeland, Erin K.] ARS, USDA, Pest Management Res Unit, Sidney, MT USA. [Meimberg, Harald] Univ Porto, CIBIO, Ctr Invest Biodiversidade & Recursos Genet, Vairao, Portugal. [Rice, Kevin J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Rice, Kevin J.] Univ Calif Davis, Ctr Populat Biol, Davis, CA 95616 USA. RP Dyer, AR (reprint author), Univ S Carolina, Dept Biol & Geol, Aiken, SC USA. EM andyd@usca.edu RI McKay, John/K-3875-2012 OI McKay, John/0000-0003-4311-5513 FU USDA NRI [2005-35320-15314] FX The authors would like to thank Carol Lee, Kristina Schierenbeck and Robert Holt for organizing a symposium titled 'Synthesis of the Ecology and Evolution of Invasive Species' and Hsein Easlon, Angel Moseley and Neil Willits for help with data collection and analyses. This study benefited greatly from the keen editorial eyes of Carol Lee and two anonymous reviewers. The Aegilops triuncialis research was supported by USDA NRI grant #2005-35320-15314 to J. K. McKay and K. J. Rice. NR 83 TC 41 Z9 41 U1 10 U2 76 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD MAR PY 2010 VL 3 IS 2 BP 179 EP 192 DI 10.1111/j.1752-4571.2010.00118.x PG 14 WC Evolutionary Biology SC Evolutionary Biology GA 561CK UT WOS:000274952800010 PM 25567918 ER PT J AU Marsico, TD Burt, JW Espeland, EK Gilchrist, GW Jamieson, MA Lindstrom, L Roderick, GK Swope, S Szucs, M Tsutsui, ND AF Marsico, Travis D. Burt, Jennifer W. Espeland, Erin K. Gilchrist, George W. Jamieson, Mary A. Lindstrom, Leena Roderick, George K. Swope, Sarah Szucs, Marianna Tsutsui, Neil D. TI Underutilized resources for studying the evolution of invasive species during their introduction, establishment, and lag phases SO EVOLUTIONARY APPLICATIONS LA English DT Review DE biological control; evolution; horticultural invasion; invasive species; lag phase; natural history collections; specimens ID BIOLOGICAL-CONTROL INTRODUCTIONS; PLANT INVASIONS; UNITED-STATES; NEW-ZEALAND; BIODIVERSITY INFORMATICS; HARMONIA-AXYRIDIS; PROPAGULE SIZE; NORTH-AMERICA; ECOLOGICAL GENETICS; POPULATION BIOLOGY AB The early phases of biological invasions are poorly understood. In particular, during the introduction, establishment, and possible lag phases, it is unclear to what extent evolution must take place for an introduced species to transition from established to expanding. In this study, we highlight three disparate data sources that can provide insights into evolutionary processes associated with invasion success: biological control organisms, horticultural introductions, and natural history collections. All three data sources potentially provide introduction dates, information about source populations, and genetic and morphological samples at different time points along the invasion trajectory that can be used to investigate preadaptation and evolution during the invasion process, including immediately after introduction and before invasive expansion. For all three data sources, we explore where the data are held, their quality, and their accessibility. We argue that these sources could find widespread use with a few additional pieces of data, such as voucher specimens collected at certain critical time points during biocontrol agent quarantine, rearing, and release and also for horticultural imports, neither of which are currently done consistently. In addition, public access to collected information must become available on centralized databases to increase its utility in ecological and evolutionary research. C1 [Marsico, Travis D.] Mississippi State Univ, Dept Biol Sci, Mississippi State, MS 39762 USA. [Burt, Jennifer W.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Espeland, Erin K.] USDA ARS, No Plains Agr Res Lab, Sidney, MT USA. [Gilchrist, George W.] Coll William & Mary, Dept Biol, Williamsburg, VA 23185 USA. [Jamieson, Mary A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Lindstrom, Leena] Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland. [Roderick, George K.; Tsutsui, Neil D.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Swope, Sarah] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Swope, Sarah] USDA, Exot & Invas Weeds Res Unit, Albany, CA USA. [Szucs, Marianna] Univ Idaho, Moscow, ID 83843 USA. RP Marsico, TD (reprint author), Arkansas State Univ, Dept Biol Sci, POB 599, State Univ, AR 72467 USA. EM tmarsico@astate.edu RI Lindstrom, Leena/B-2326-2012; OI Lindstrom, Leena/0000-0002-6452-4538; Jamieson, Mary/0000-0002-1716-0372 FU US Geological Survey Biological Resources Discipline [04HQAG0135, 08HQAG0139]; US Department of Agriculture [2007-55320-17847]; US Department of Agriculture NRI-CSREES [2008-35302-04680]; Academy of Finland [118456] FX We would like to thank C. Lee, K. Schierenbeck, and R. Holt, organizers of 'Synthesizing Ecology and Evolution for the Study of Invasive Species,' for putting together a great meeting that spawned our collaboration. We also thank the other conference participants who provided insightful comments. We thank E. Coombs (Oregon State Department of Agriculture), J. Ertle (USDA ARS BIRL), G. Hanes (USDA ARS BCDC), and J. Milan (ISDA/BLM) for providing invaluable insider information on the present state of biological control recordkeeping. We thank S. Reichard (University of Washington) and A. Tschanz (USDA APHIS) for their insights on the current documentation of horticultural imports. We thank J. Skarstad for information on seed catalog holdings digitization. T.D.M. was funded under grants to G.N. Ervin from the US Geological Survey Biological Resources Discipline (04HQAG0135 and 08HQAG0139) and US Department of Agriculture (2007-55320-17847) during the writing of this manuscript. This work also was completed with the help of funding from the US Department of Agriculture NRI-CSREES grant 2008-35302-04680 to N.D.T. and from the Academy of Finland (project 118456) to L.L. We thank A. Caesar, D. Kazmer, and K. Rice who commented on an earlier draft of the manuscript and two anonymous referees who provided valuable suggestions that improved the study. NR 118 TC 22 Z9 22 U1 12 U2 78 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD MAR PY 2010 VL 3 IS 2 BP 203 EP 219 DI 10.1111/j.1752-4571.2009.00101.x PG 17 WC Evolutionary Biology SC Evolutionary Biology GA 561CK UT WOS:000274952800012 PM 25567920 ER PT J AU Nakata, PA He, CX AF Nakata, Paul A. He, Cixin TI Oxalic acid biosynthesis is encoded by an operon in Burkholderia glumae SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE transposon; oxalate; polycistronic ID FUNGUS TYROMYCES-PALUSTRIS; ASPERGILLUS-NIGER; OXALOACETATE HYDROLASE; OXALATE PRODUCTION; GRAIN ROT; RICE; SOLUBILIZATION; PURIFICATION; PATHOGEN AB Although the biosynthesis of oxalic acid is known to occur in a number of bacteria, the mechanism(s) regulating its production remains largely unknown. To date, there is no report on the identification of an oxalic acid biosynthetic pathway gene from bacteria. In an attempt to identify such a gene(s), a mutant screen was conducted using the simple oxalic acid-producing phytopathogenic bacterium, Burkholderia glumae. Four mutants that failed to produce oxalic acid were isolated from a transposon-mutagenized B. glumae library and named Burkholderia oxalate defective (Bod)1. DNA sequence analysis revealed that each mutant contained an insertion event at different sites in the same ORF, which we referred to as the oxalate biosynthetic component (obc)A locus. Complementation of the Bod1 mutant with the obcA gene, however, resulted only in a partial restoration of the oxalic acid-producing phenotype. Further complementation studies utilizing a larger DNA fragment encompassing the obcA locus coupled with deletion mutagenesis led to the identification of another ORF that we named the obcB locus, which was essential for higher levels of oxalic acid production. Transcript analysis indicated that both obcA and obcB are coexpressed and encoded on a single polycistron message. C1 [Nakata, Paul A.; He, Cixin] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Nakata, PA (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM pnakata@bcm.tmc.edu FU US Department of Agriculture; Agricultural Research Service [58-6250-6-001] FX The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. This research was supported in part by the US Department of Agriculture, Agricultural Research Service, under Cooperative agreement number 58-6250-6-001. Thanks are due to Keri Wang for providing the pRK415 vector and Michele McConn, John Knight, and Ross Holmes for their comments on the manuscript. NR 27 TC 14 Z9 14 U1 0 U2 8 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0378-1097 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD MAR PY 2010 VL 304 IS 2 BP 177 EP 182 DI 10.1111/j.1574-6968.2010.01895.x PG 6 WC Microbiology SC Microbiology GA 555BP UT WOS:000274482300010 PM 20141533 ER PT J AU Gurusinghe, D Gill, S Almario, RU Lee, J Horn, WF Keim, NL Kim, K Karakas, SE AF Gurusinghe, Dumindra Gill, Sharan Almario, Rogelio U. Lee, Jennifer Horn, William F. Keim, Nancy L. Kim, Kyoungmi Karakas, Sidika E. TI In polycystic ovary syndrome, adrenal steroids are regulated differently in the morning versus in response to nutrient intake SO FERTILITY AND STERILITY LA English DT Article DE Adrenal steroids; PCOS; obesity; insulin resistance; leptin ID ASYMPTOMATIC REACTIVE HYPOGLYCEMIA; INSULIN-RESISTANCE; ANDROGEN LEVELS; OBESE SUBJECTS; LEPTIN LEVELS; METFORMIN TREATMENT; EUMENORRHEIC WOMEN; CONTROLLED-TRIAL; GROWTH-HORMONE; PLASMA LEPTIN AB Objective: To investigate adrenal steroid regulation in polycystic ovary syndrome. Design: Five-hour oral glucose tolerance test (OGTT) and frequently sampled-intravenous glucose tolerance test. Setting: University research center. Patient(s): Thirty patients. Intervention(s): None. Main Outcome Measure(S): Anthropometrics, leptin, cortisol, DHEAS, glucose, insulin. Result(s): Morning cortisol correlated with sensitivity index (SI, r =.540), DHEAS correlated inversely with age (r = -.6359), body mass index (BMI, r = -.6199), fat mass (r = -0.630), and leptin (r = -0.5676). Between the second and fourth hour of OGTT, cortisol changes (A) exhibited three patterns: 1, responders (n = 9, Delta: 10.7 +/- 1.0 mu g/dL; II, nonresponders (n = 10, Delta: -3.5 +/- 0.6 mu g/dL); III, intermediates (n = 11, Delta: 4.3 +/- 1.0 mu g/dL). Compared with nonresponders, responders were more obese (BMI: 37.0 +/- 1.6 vs. 31.7 +/- 1.8 kg/m(2)); had higher leptin (28.9 +/- 1.7 vs. 24.1 +/- 1.1 ng/mL), and lower DHEAS (133 +/- 12 vs. 236 +/- 32 ng/mL), higher glucose at 1 h of OGTT (195 +/- 13 vs. 131 +/- 12 mg/dL), higher area under the curve (AUC)(Glucose) (332 +/- 20 vs. 265 +/- 17 mg/dL), higher AUC(Insulin) (244 +/- 50 vs. 125 +/- 30 mu U/mL), and lower nadir glucose (61 2 vs. 70 2 mg/dL). Conclusion(s): Obesity and insulin resistance are associated with lower morning cortisol and DHEAS but increased cortisol and DHEA responses after glucose ingestion. Morning steroid levels may not reflect the day-long exposure. (Fertil Steril (R) 2010;93:1192-9. (C)2010 by American Society for Reproductive Medicine.) C1 [Gurusinghe, Dumindra; Gill, Sharan; Almario, Rogelio U.; Lee, Jennifer; Karakas, Sidika E.] Univ Calif Davis, Div Endocrinol Clin Nutr & Vasc Med, Dept Internal Med, Sacramento, CA 95817 USA. [Karakas, Sidika E.] No Calif Hlth Care Syst, Dept Vet Affairs, Mather, CA USA. [Horn, William F.; Keim, Nancy L.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Kim, Kyoungmi] Univ Calif Davis, Div Biostat, Dept Publ Hlth Sci, Sch Med, Sacramento, CA 95817 USA. RP Karakas, SE (reprint author), Univ Calif Davis, Div Endocrinol Clin Nutr & Vasc Med, Dept Internal Med, 4150 V St,PSSB Suite G400, Sacramento, CA 95817 USA. EM sekarakas@ucdavis.edu OI Horn, William/0000-0002-2681-4163 FU National Center for Complementary and Alternative Medicine [R21 AT002280]; ALSAM Foundation; UC Davis Clinical and Translational Science Center [FIR 024146] FX Supported by grants from the National Center for Complementary and Alternative Medicine (R21 AT002280) and the ALSAM Foundation to Dr. Kasim-Karakas. The clinical studies were partially supported by the UC Davis Clinical and Translational Science Center Grant (FIR 024146). NR 43 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0015-0282 EI 1556-5653 J9 FERTIL STERIL JI Fertil. Steril. PD MAR 1 PY 2010 VL 93 IS 4 BP 1192 EP 1199 DI 10.1016/j.fertnstert.2009.03.017 PG 8 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA 568RT UT WOS:000275541000024 PM 19342030 ER PT J AU Yeh, HY Klesius, PH AF Yeh, Hung-Yueh Klesius, Phillip H. TI Sequence analysis, characterization and tissue distribution of channel catfish (Ictalurus punctatus Rafinesque, 1818) myeloperoxidase cDNA SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Channel catfish; Ictalurus punctatus; Myeloperoxidase; MPO; Edwardsiella ictaluri ID MOLECULAR-CLONING; FUNCTIONAL-CHARACTERIZATION; GENOMIC ORGANIZATION; EXPRESSION ANALYSIS; HUMAN-NEUTROPHILS; PEROXIDASE; GENE; PHAGOCYTES; HETEROGENEITY; POPULATIONS AB Myeloperoxidase (EC 111 17), a heme-containing lysosomal glycoprotein, is found predominantly in azurophilic granules of neutrophils This enzyme upon activation catalyzes hydrogen peroxide in the presence of various halide ions to form hypohalous acids Subsequently. these reagents are able to kill the invading microorganisms. In this study, we report the identification, characterization and expression analysis of the channel catfish myeloperoxidase transcript. The full-length nucleotide sequence of channel catfish myeloperoxidase cDNA had 3157 nucleotides, including an open reading frame, which appears to encode a putative peptide of 771 amino acid residues with a calculated molecular mass of 87.14 kDa By comparison with the human counterpart, the channel catfish myeloperoxidase peptide can be divided into domains and has conservative features, including peroxidase catalytic sites, covalent linkage sites for the heme group and all cysteine residues. The channel catfish myeloperoxidase transcript was detected by RT-PCR in anterior kidneys, where the major leukocyte population is neutrophil precursors Reagent development and the role of this enzyme in Edwardsiella ictaluri infection are under investigation Published by Elsevier Ltd C1 [Yeh, Hung-Yueh; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Yeh, HY (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. FU USDA Agricultural Research Service CRIS [6420-32000-020-00D] FX This study Was Supported by the USDA Agricultural Research Service CRIS project no. 6420-32000-020-00D Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U S. Department of Agriculture. NR 48 TC 6 Z9 6 U1 2 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD MAR PY 2010 VL 28 IS 3 BP 504 EP 509 DI 10.1016/j.fsi.2009.12.007 PG 6 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA 570UX UT WOS:000275704900014 PM 20034575 ER PT J AU Rothlisberger, JD Chadderton, WL McNulty, J Lodge, DM AF Rothlisberger, John D. Chadderton, W. Lindsay McNulty, Joanna Lodge, David M. TI Aquatic Invasive Species Transport via Trailered Boats: What Is Being Moved, Who Is Moving It, and What Can Be Done SO FISHERIES LA English DT Article ID LAKE-ST-CLAIR; INLAND LAKES; ZEBRA MUSSEL; BIOLOGICAL INVASIONS; EURASIAN WATERMILFOIL; DREISSENA-POLYMORPHA; GRAVITY MODELS; DISPERSAL; MANAGEMENT; SPREAD AB Trailered boats have been implicated in the spread of aquatic invasive species. There has been, however, little empirical research on the type and quantity of aquatic invasive species being transported, nor on the efficacy of management interventions (e. g., inspection crews, boat washing). In a study of small-craft boats and trailers, we collected numerous aquatic and terrestrial organisms, including some species that are morphologically similar to known aquatic invasive species. Additionally, a mail survey of registered boaters (n = 944, 11% response rate) and an in-person survey of boaters in the field (n = 459, 90% response rate) both indicated that more than two-thirds of boaters do not always take steps to clean their boats. Furthermore, we used a controlled experiment to learn that visual inspection and hand removal can reduce the amount of macrophytes on boats by 88% +/- 5% (mean +/- SE), with high-pressure washing equally as effective (83% +/- 4%) and low-pressure washing less so (62% +/- 3% removal rate). For removing small-bodied organisms, high-pressure washing was most effective with a 91% +/- 2% removal rate; low-pressure washing and hand removal were less effective (74% +/- 6% and 65% +/- 4% removal rates, respectively). This research supports the widespread belief that trailered boats are an important vector in the spread of aquatic invasive species, and suggests that many boaters have not yet adopted consistent and effective boat cleaning habits. Therefore, additional management efforts may be appropriate. C1 [Rothlisberger, John D.; McNulty, Joanna; Lodge, David M.] Univ Notre Dame, Ctr Aquat Conservat, Notre Dame, IN 46556 USA. [Rothlisberger, John D.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. RP Rothlisberger, JD (reprint author), US Forest Serv, USDA, Milwaukee, WI USA. EM jrothlisberger@fs.fed.us RI Rothlisberger, John/J-9777-2012 FU National Science Foundation [DEB 02-13698]; Great Lakes Protection Fund [797]; University of Notre Dame Schmitt FX We thank Mark Fedora, Nick Schmal, and Mike Ielmini of the U. S. Forest Service for their help in organizing this project. Sheilah Kennedy and Jeff and Richard Delfeld of SK-Environmental (Okanogan, Washington) furnished the portable wash-reclaim system and gave essential technical support and field assistance. Ted Ritter, AIS coordinator for Vilas County, Wisconsin, provided invaluable logistical and field support. Special thanks are due to Jody Peters for help identifying macrophytes and to Kevin Pangle for supplying Bythotrephes for our experiments. Elizabeth D. Tucker helped to design the mail survey. Reuben Keller's help was crucial to the successful implementation of the in-person surveys. We are grateful to Brandon Feasel and Neil Wallace for personally interviewing scores of boaters and to Matt Barnes, Mike McCann, Sarah Sutton, and Tim Campbell for their tireless laboratory work. Funding from the National Science Foundation for the ISIS project (DEB 02-13698 to DML), the Great Lakes Protection Fund (Grant #797 to DML), and a University of Notre Dame Schmitt Fellowship (to JDR) made this work possible. NR 39 TC 47 Z9 48 U1 6 U2 43 PU AMER FISHERIES SOC PI BETHESDA PA 5410 GROSVENOR LANE SUITE 110, BETHESDA, MD 20814-2199 USA SN 0363-2415 J9 FISHERIES JI Fisheries PD MAR PY 2010 VL 35 IS 3 BP 121 EP 132 DI 10.1577/1548-8446-35.3.121 PG 12 WC Fisheries SC Fisheries GA 588ZN UT WOS:000277113500003 ER PT J AU Howard, FW Pemberton, R Schroer, S Hodges, G AF Howard, F. W. Pemberton, Robert Schroer, Sibylle Hodges, Gregory TI PARATACHARDINA PSEUDOLOBATA (COCCOIDEA: KERRIIDAE): BIONOMICS IN FLORIDA SO FLORIDA ENTOMOLOGIST LA English DT Article DE lobate lac scale; bionomics; Florida; parasitism ID LOBATE LAC SCALE; HOST ACCEPTANCE TRIALS; BIOLOGICAL-CONTROL; HEMIPTERA; PEST; INFESTATION; PARASITOIDS; CHAMBERLIN AB The lobate lac scale, Paratachardina pseudolobata Kondo & Gullan, attacks large numbers of plants in Florida. The scales primarily infest the branches and main stems <2 cm in diameter; rarely were they found on stems that were larger than 4 cm in diameter or on leaves and never on roots. They produce honeydew, but ants did not tend the scales nor did ants appear attracted to the honeydew, which is ejected from the scale instead of being present in collectable droplets. Males have not been found in Florida populations of the lobate lac scale and females are parthenogenetic and ovoviviparous. The scales developed into adult females in 15-19 weeks. Crawlers of second generation were present 21 weeks after young crawlers of the previous generation were placed on a host plant. The pre-imaginal stadia were: first instar 8-11 weeks; second instar 7-8 weeks. The numbers of crawlers counted per month on host plants were notably uniform throughout the year, but there was evidence of a slight increase in crawler production during the warmer months. Lobate lac scales subjected to a simulated frost of -3 degrees C had a low survival rate. In a test of survival without a host, one of 22 crawlers survived 14 d, and 3 of 32 survived 18 d. Based on the numbers of exit holes in mature female tests, the parasitism rate since this pest was found in Florida is not greater than 1%. C1 [Howard, F. W.] Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, Ft Lauderdale, FL 33314 USA. [Pemberton, Robert] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Schroer, Sibylle] Fed Res Ctr Cultivated Plants, JKI, Inst Strategies & Technol Assessment Plant Protec, D-14532 Kleinmachnow, Germany. [Hodges, Gregory] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. RP Howard, FW (reprint author), Univ Florida, Ft Lauderdale Res & Educ Ctr, IFAS, 3205 Coll Ave, Ft Lauderdale, FL 33314 USA. FU Florida Nursery and Growers Association; South Florida Water Management District; USDA-APHIS FX We thank Bryan Steinberg, Sebastian Ortiz, Sergio Gallo, and Nancy Miller, University of Florida Fort Lauderdale Research & Education Center, for assistance with this work. The research was financially supported by the Florida Nursery and Growers Association, the South Florida Water Management District, and the USDA-APHIS. NR 20 TC 4 Z9 4 U1 0 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 1 EP 7 PG 7 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200001 ER PT J AU Halbert, SE Manjunath, KL Ramadugu, C Brodie, MW Webb, SE Lee, RF AF Halbert, Susan E. Manjunath, Keremane L. Ramadugu, Chandrika Brodie, Matthew W. Webb, Susan E. Lee, Richard F. TI TRAILERS TRANSPORTING ORANGES TO PROCESSING PLANTS MOVE ASIAN CITRUS PSYLLIDS SO FLORIDA ENTOMOLOGIST LA English DT Article DE Diaphorina citri Kuwayama; Asian citrus psyllid; citrus greening disease; huanglongbing; Citrus; fruit movement ID DIAPHORINA-CITRI; DISEASE; FLORIDA AB Huanglongbing (citrus greening) is one of the most serious diseases of citrus. Movement of the disease occurs as a result of natural transmission by the insect vector and by movement of infected plant material. We demonstrate here that Diaphorina citri Kuwayama, the vector of citrus greening pathogens, can be transported in trailers of unprocessed fruit. Several samples of D. citri collected from trailers of oranges were positive for citrus greening pathogens, indicating that the disease can spread widely with the movement of the mature fruit as a result of moving the vectors. While movement of disease and the vector through plant materials into new areas has been recognized, our findings emphasize the need to evaluate the importance of long distance movement of psyllids on unprocessed fruit, even in the absence of vegetative tissue. C1 [Halbert, Susan E.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA. [Manjunath, Keremane L.; Ramadugu, Chandrika; Lee, Richard F.] ARS, USDA, Riverside, CA 92507 USA. [Brodie, Matthew W.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Naples, FL USA. [Webb, Susan E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Halbert, SE (reprint author), Florida Dept Agr & Consumer Serv, Div Plant Ind, POB 147100, Gainesville, FL 32614 USA. NR 16 TC 17 Z9 17 U1 1 U2 12 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 33 EP 38 PG 6 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200004 ER PT J AU Lee, JH Pemberton, RW AF Lee, Jang-Hoon Pemberton, Robert W. TI PUPAL PARASITOIDS OF YPONOMEUTA MALINELLUS (LEPIDOPTERA: YPONOMEUTIDAE) IN NORTHEAST ASIA SO FLORIDA ENTOMOLOGIST LA English DT Article DE apple; biological control; China; Japan; Korea; Malus; parasitoid; Yponomeuta malinellus ID APPLE ERMINE MOTH; BIOLOGICAL-CONTROL; KOREA; ICHNEUMONIDAE; HYMENOPTERA; PHENOLOGY; NORTHWEST; ZELLER AB Pupal parasitism of the apple ermine moth, Yponomeuta malinellus Zeller (Lepidoptera: Yponomeutidae), was investigated in northeast Asia with the goal of identifying potential biological controls of the moth, which threatened the apple industry in Washington State, USA during the 1980s. Seven primary and 2 secondary parasitoids were recorded from 27, 472 pupae collected and reared from a total of 20 collections at 16 sites in Korea, northern Honshu and Hokkaido Islands in Japan, and Shanxi Province in China during a 4-year period from 1988 to 1991. The mean total parasitism rate was highest in Korea (38.5%), while the lowest rate of 2.1% was recorded from Shanxi, China. The mean total parasitism rate detected from Hokkaido and Honshu Islands in Japan was 17.2 and 10.1%, respectively. The ichnueumonid wasp Herpestomus brunnicornis Gravenhorst dominated the parasitism in Korea and Japan, while Coccygomimus disparis (Viereck) was responsible for most mortality of the moth pupae in Shanxi, China. Diadegma armillatum (Gravenhorst) caused very low mortality in this study, although it is known as an important parasitoid from Europe and Eurasia. There were significantly different levels of parasitism rates of apple ermine moth pupae among geographical locations sampled, likely due to the habitat type and host plant of the apple ermine moth. Herpestomus brunnicornis collected from Korea and Japan was established in Washington State, USA as a biocontrol agent of the apple ermine moth. C1 [Pemberton, Robert W.] USDA ARS, Asian Parasite Lab, Seoul, South Korea. RP Pemberton, RW (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. NR 24 TC 0 Z9 0 U1 3 U2 6 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 45 EP 51 PG 7 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200006 ER PT J AU Lapointe, SL Niedz, RP Evens, TJ AF Lapointe, Stephen L. Niedz, Randall P. Evens, Terence J. TI AN ARTIFICIAL DIET FOR DIAPREPES ABBREVIATUS (COLEOPTERA: CURCULIONIDAE) OPTIMIZED FOR LARVAL SURVIVAL SO FLORIDA ENTOMOLOGIST LA English DT Article DE Diaprepes root weevil; artificial diet; response surface model; mixture optimization ID BIOLOGY; WEEVIL AB The root weevil Diaprepes abbreviatus (L.) has been reared since 1992 on an artificial diet first reported in 1982. Recently, we have shown that several ingredients included in the original diet have little or no effect on insect performance. Here we examined the effects of 2 principal drivers (cottonseed meal and wheat germ) on weight gain and survival of larval D. abbreviatus in varying proportions along with a non-nutritive filler (cellulose). We employed a geometric design to evaluate amount and proportion combined with response surface models to identify optimal proportions for larval weight gain, larval survival, and development rate. All larval responses measured lead to the conclusion that cottonseed meal is the only major nutritive component, in addition to standard vitamin and salt mixes, required for a successful artificial diet for rearing larvae of D. abbreviatus to pupation. C1 [Lapointe, Stephen L.; Niedz, Randall P.; Evens, Terence J.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Lapointe, SL (reprint author), USDA ARS, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. NR 14 TC 1 Z9 1 U1 1 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 56 EP 62 PG 7 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200008 ER PT J AU Meagher, RL Landolt, PJ AF Meagher, Robert L. Landolt, Peter J. TI BINARY FLORAL LURE ATTRACTIVE TO VELVETBEAN CATERPILLAR ADULTS (LEPIDOPTERA: NOCTUIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE floral lure; attractant; trapping; moths; Anticarsia gemmatalis ID CABBAGE-LOOPER MOTHS; ALFALFA LOOPER; WINTER SURVIVAL; SEX-PHEROMONE; IDENTIFICATION; FIELD; PHENYLACETALDEHYDE; FLOWERS; SYSTEM; TRAPS AB Evaluation of combinations of flower odor compounds in northern Florida revealed that linalool was synergistic in attractiveness with phenylacetaldehyde (PAA) to the migratory moth velvetbean caterpillar (Anticarsia gemmatalis Hubner). This noctuid was the most common species collected from traps with a binary lure composed of PAA and linalool, with over 900 males and females collected in Sep 2005 and almost 13,000 collected in a 4-week period in Aug and Sep 2006. Different lid openings of the vials containing the chemical blend in an attempt to vary the release rate of the binary lure did not affect the number of velvetbean caterpillar moths captured. Traps baited with the binary lure and placed in May, Jun and early Jul of 2005 and 2009 failed to capture adults demonstrating the absence of adult moths early in the season. Management application of floral attractants in an attract-and-kill strategy is discussed. C1 [Meagher, Robert L.] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. [Landolt, Peter J.] ARS, USDA, Wapato, WA 98951 USA. RP Meagher, RL (reprint author), ARS, USDA, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. NR 42 TC 0 Z9 6 U1 3 U2 9 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 73 EP 79 PG 7 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200010 ER PT J AU Allen, HR Valles, SM Miller, DM AF Allen, Hamilton R. Valles, Steven M. Miller, Dim M. TI CHARACTERIZATION OF SOLENOPSIS INVICTA (HYMENOPTERA: FORMICIDAE) POPULATIONS IN VIRGINIA: SOCIAL FORM GENOTYPING AND PATHOGEN/PARASITOID DETECTION SO FLORIDA ENTOMOLOGIST LA English DT Article DE monogyne; polygyne; Kneallhazia solenopsae; Solenopsis invicta virus-1; Pseudacteon ID IMPORTED FIRE ANT; MULTIPLEX PCR; MICROSPORIDIA; THELOHANIA; POLYGYNE; FLORIDA; RANGE; PHORIDAE; COLONIES; HABITAT AB Red imported fire ant, Solenopsis invicta Buren, workers were sampled from 26 colonies in Virginia during the 2007-2008 time period. Polymerase chain reaction (PCR) assays were used to determine colony social form (monogyny or polygyny) by genotyping ants at the Gp-9 locus. Twenty of the colonies (76.9%) were found to be polygyne. Multiplex PCR was also used to detect the presence of several organisms currently being used as biological control agents for fire ants in the U.S., including the microsporidian parasite Kneallhazia solenopsae and Pseudacteon spp. parasitioid decapitating phorid flies in the sampled colonies. Kneallhazia solenopsae was detected in 11 of 26 colonies (42%). In addition, Pseudacteon spp. flies were detected in 2 (7.7%) colonies. The sampled colonies were examined by reverse transcription PCR (RT-PCR) for the presence of Solenopsis invicta viruses -1 and -2. Results indicated that 5 colonies were infected with SINV-1 (19%) and none were infected with SINV-2. This study is the first to characterize the red imported fire ant infestation in Virginia and documents the presence of biological control agents in this area. C1 [Allen, Hamilton R.; Miller, Dim M.] Virginia Tech, Dept Entomol, Blacksburg, VA 24060 USA. [Valles, Steven M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Allen, HR (reprint author), Virginia Tech, Dept Entomol, 216A Price Hall, Blacksburg, VA 24060 USA. NR 33 TC 4 Z9 4 U1 3 U2 15 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 80 EP 88 PG 9 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200011 ER PT J AU Porter, SD AF Porter, Sanford D. TI DISTRIBUTION OF THE FORMOSA STRAIN OF THE FIRE ANT DECAPITATING FLY PSEUDACTEON CURVATUS (DIPTERA: PHORIDAE) THREE AND A HALF YEARS AFTER RELEASES IN NORTH FLORIDA SO FLORIDA ENTOMOLOGIST LA English DT Article DE Solenopsis invicta; red imported fire ant; phorid fly; biological control; parasite ID HOST-SPECIFICITY; FLIES DIPTERA; FIELD RELEASE; SOUTH-AMERICA; PARASITOIDS; ESTABLISHMENT; HYMENOPTERA; FORMICIDAE; TRICUSPIS; DISPERSAL AB The Formosa biotype of the decapitating fly Pseudacteon curvatus Borgmeier was released and successfully established as a self-sustaining biocontrol agent of the red imported fire ant Solenopsis invicta Buren at several sites around Gainesville, FL in 2003. In order to determine the status of these releases, 59 sites were surveyed for flies in the fall of 2006 with sticky traps and aspirators. Results of this survey showed that the flies had expanded outward an average of 74 km and occupied more than 30,000 km(2) in North Central Florida. This rate of expansion was faster than rates reported for Pseudacton tricuspis Borgmeier in Florida, Louisiana, and Texas. The rapid rates of expansion and high densities reported for the Formosa biotype of R curvatus indicate that it is a vigorous parasitoid which will require relatively fewer field releases to establish it as biocontrol agent of red imported fire ants in the United States. C1 USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Porter, SD (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 29 TC 15 Z9 15 U1 3 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 107 EP 112 PG 6 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200014 ER PT J AU Zhang, JL Wheeler, GS Purcell, M Ding, JQ AF Zhang, Jialiang Wheeler, Gregory S. Purcell, Matthew Ding, Jianqing TI BIOLOGY, DISTRIBUTION, AND FIELD HOST PLANTS OF MACROPLEA JAPANA IN CHINA: AN UNSUITABLE CANDIDATE FOR BIOLOGICAL CONTROL OF HYDRILLA VERTICILLATA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID HYDRELLIA-PAKISTANAE DIPTERA; HYDROCHARITACEAE; EPHYDRIDAE C1 [Zhang, Jialiang; Ding, Jianqing] Chinese Acad Sci Moshan, Wuhan Bot Garden Inst, Invas Ecol & Biocontrol Lab, Wuhan 430074, Hubei Province, Peoples R China. [Wheeler, Gregory S.] USDA ARS Invas Plant Res, Ft Lauderdale, FL 33314 USA. [Purcell, Matthew] CSIRO Entomol, USDA ARS, Australian Biol Control Lab, Indooroopilly, Qld 4068, Australia. RP Ding, JQ (reprint author), Chinese Acad Sci Moshan, Wuhan Bot Garden Inst, Invas Ecol & Biocontrol Lab, Wuhan 430074, Hubei Province, Peoples R China. EM dingjianqing@yahoo.com RI Purcell, Matthew/C-2098-2008 NR 8 TC 4 Z9 5 U1 0 U2 3 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 116 EP 119 PG 4 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200016 ER PT J AU Hummel, NA Hardy, T Reagan, TE Pollet, D Carlton, C Stout, MJ Beuzelin, JM Akbar, W White, W AF Hummel, N. A. Hardy, T. Reagan, T. E. Pollet, D. Carlton, C. Stout, M. J. Beuzelin, J. M. Akbar, W. White, W. TI MONITORING AND FIRST DISCOVERY OF THE MEXICAN RICE BORER EOREUMA LOFTINI (LEPIDOPTERA: CRAMBIDAE) IN LOUISIANA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material C1 [Hummel, N. A.; Reagan, T. E.; Pollet, D.; Carlton, C.; Stout, M. J.; Beuzelin, J. M.; Akbar, W.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Hardy, T.] Louisiana Dept Agr & Forestry, Baton Rouge, LA USA. [White, W.] USDA ARS, Sugarcane Res Unit, Houma, LA 70361 USA. RP Hummel, NA (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, 404 Life Sci Bldg, Baton Rouge, LA 70803 USA. NR 14 TC 21 Z9 21 U1 2 U2 9 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 123 EP 124 PG 2 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200018 ER PT J AU Medal, J Bustamante, N Overholt, W Diaz, R Stansly, P Roda, A Amalin, D Hibbard, K Gaskalla, R Sellers, B Hight, S Cuda, J AF Medal, J. Bustamante, N. Overholt, W. Diaz, R. Stansly, P. Roda, A. Amalin, D. Hibbard, K. Gaskalla, R. Sellers, B. Hight, S. Cuda, J. TI BIOLOGICAL CONTROL OF TROPICAL SODA APPLE (SOLANACEAE) IN FLORIDA: POST-RELEASE EVALUATION SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material C1 [Medal, J.; Bustamante, N.; Overholt, W.; Diaz, R.; Stansly, P.; Amalin, D.; Sellers, B.; Cuda, J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Roda, A.] USDA, APHIS, PPQ, CPHST,Subtrop Hort Res Stn, Miami, FL USA. [Hibbard, K.; Gaskalla, R.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL USA. [Hight, S.] ARS, USDA, CMAVE, Tallahassee, FL USA. RP Medal, J (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. NR 6 TC 5 Z9 5 U1 0 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2010 VL 93 IS 1 BP 130 EP 132 PG 3 WC Entomology SC Entomology GA 569VJ UT WOS:000275630200021 ER PT J AU Allen, LH Rosenberg, IH Oakley, GP Omenn, GS AF Allen, Lindsay H. Rosenberg, Irwin H. Oakley, Godfrey P. Omenn, Gilbert S. TI Considering the case for vitamin B-12 fortification of flour SO FOOD AND NUTRITION BULLETIN LA English DT Article; Proceedings Paper CT 2nd Technical Workshop on Wheat Flour Fortification CY MAR 30-APR 03, 2008 CL Atlanta, GA DE Deficiency; flour; fortification; vitamin B-12 ID FOLIC-ACID FORTIFICATION; FOOD-COBALAMIN MALABSORPTION; LOW PLASMA VITAMIN-B-12; KENYAN SCHOOL-CHILDREN; METHYLMALONIC ACID; OLDER AMERICANS; HOMOCYSTEINE CONCENTRATIONS; COGNITIVE IMPAIRMENT; FORTIFIED POPULATION; STATUS INDICATORS AB Reasons to fortify flour with vitamin B-12 are considered, including the high prevalence of depletion and deficiency of this vitamin that occurs in persons of all ages in resource-poor countries and in the elderly in wealthier countries, and the adverse functional consequences of poor vitamin B-12 status From a global perspective, the main cause of inadequate intake and status is a low intake of animal-source foods; even lacto-ovo vegetarians have lower serum vitamin B-12 concentrations than omnivores, and far various reasons many populations have limited consumption of animal-source foods Infants are vitamin B-12-depleted from early infancy if their mothers' vitamin B-12 status and intake are poor during pregnancy and lactation Even in the United States, more than 20% of the elderly have serum vitamin B-12 concentrations that indicate depletion, and an additional 6% have deficiency, primarily due to gastric atrophy, which impairs the absorption of the vitamin from food but usually not from supplements or fortified foods Although the evidence is limited, it shows that fortified flour, consumed as bread, can improve vitamin B-12 status. Where vitamin B-12 fortification is implemented, the recommendation is to add 20 mu g/kg flour, assuming consumption of 75 to 100 g flour per day, to provide 75% to 100% of the Estimated Average Requirement; the amount of the vitamin that can be added is limited by its cost. The effectiveness of this level of addition for improving vitamin B-12 status in programs needs to be determined and monitored In addition, further research should evaluate the bioavailability of the vitamin from fortified flour by elderly people with food cobalamin malabsorption and gastric atrophy. C1 [Allen, Lindsay H.] Univ Calif Davis, ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Rosenberg, Irwin H.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Oakley, Godfrey P.] Emory Univ, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA. [Omenn, Gilbert S.] Univ Michigan, Ann Arbor, MI 48109 USA. RP Allen, LH (reprint author), Univ Calif Davis, ARS, USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. NR 70 TC 20 Z9 20 U1 1 U2 13 PU INT NUTRITION FOUNDATION PI BOSTON PA 150 HARRISON AVE, BOSTON, MA 02111 USA SN 0379-5721 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD MAR PY 2010 VL 31 IS 1 SU S BP S36 EP S46 PG 11 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 588WH UT WOS:000277104800004 PM 20629351 ER PT J AU Sutivisedsak, N Cheng, HN Willett, JL Lesch, WC Tangsrud, RR Biswas, A AF Sutivisedsak, N. Cheng, H. N. Willett, J. L. Lesch, W. C. Tangsrud, R. R. Biswas, Atanu TI Microwave-assisted extraction of phenolics from bean (Phaseolus vulgaris L.) SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE Beans; Phenolics; Microwave; Solvent extraction ID ANTIOXIDANT ACTIVITY; SEED COAT; CAPACITY; FLAVONOIDS; CULTIVARS; COLOR AB Phenolic phytochemicals are associated with many health benefits, and it would be useful to develop improved methods for their extraction from bean. In this work, we showed that microwave-assisted extraction was an effective method. Eight bean types important in the Northarvest region of North America, which includes North Dakota and Minnesota, were chosen for this study. Four temperatures (25, 50, 100 and 150 degrees C) and three solvents (water, 50% ethanol in water and 100% ethanol) were investigated. As expected, colored beans contained higher concentrations of extractable phenolics, and in most cases the concentration of phenolics was much higher in the hull (testa) than in the cotyledon. Extraction efficiency was superior at higher extraction temperatures. The most effective extraction was achieved at a temperature of 150 degrees C using 50% ethanol. Total phenolics contents determined by microwave-assisted extraction with water at 100 degrees C were two to three times those determined by conventional extraction with water at the same temperature. Published by Elsevier Ltd. C1 [Sutivisedsak, N.; Willett, J. L.; Biswas, Atanu] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Cheng, H. N.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Lesch, W. C.; Tangsrud, R. R.] Univ N Dakota, Grand Forks, ND 58202 USA. RP Biswas, A (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM atanu.biswas@ars.usda.gov FU Northarvest Bean Growers Association FX This study Was Supported by a grant from the Northarvest Bean Growers Association. The authors express their heartfelt thanks to Janet Berfield for assistance with microwave extraction, to Dr. Roque Evangelista, Billy Deadmond and PaUllete Smith for assistance with bean clehulling, and to Dr. Devin Rose for statistical analysis. NR 23 TC 25 Z9 26 U1 6 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 J9 FOOD RES INT JI Food Res. Int. PD MAR PY 2010 VL 43 IS 2 SI SI BP 516 EP 519 DI 10.1016/j.foodres.2009.09.014 PG 4 WC Food Science & Technology SC Food Science & Technology GA 563TW UT WOS:000275158400013 ER PT J AU Berrios, JD Morales, P Camara, M Sanchez-Mata, MC AF Berrios, J. De J. Morales, P. Camara, M. Sanchez-Mata, M. C. TI Carbohydrate composition of raw and extruded pulse flours SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE Extrusion; Pulses; Carbohydrates; alpha-Galactosides; Oligosaccharides ID OLIGOSACCHARIDE CONTENT; DIETARY FIBER; LEGUME SEEDS; FLATULENCE; EXTRUSION; STARCH; FRACTIONS; REDUCTION; CICERITOL; COOKING AB Extrusion cooking technology is commercially used in the fabrication of a variety of snack-type and ready-to-eat foods made from cereal grains. However, with the exception of soybean, pulses such as lentil, dry pea and chickpea have not been used for the development of extruded food products. In this study, total carbohydrates, mono-, di- and oligosaccharides, and soluble and insoluble dietary fiber were determined before and after extrusion cooking under specific processing conditions. Concentrations of total available carbohydrates (TAC) in lentil, chickpea and dry pea flours ranged from 625 g/kg to 657 g/kg dry matter. Dry pea showed the highest concentration of TAC, followed by chickpea and lentil. Extrusion processing did not significantly (p < 0.05) affect the TAC content of dry pea and lentil flours. However, extrusion processing decreased the concentration of the raffinose family of oligosaccharides (raffinose and stachyose) in pulse extrudates. Formulated pulse flours demonstrated a beneficial increase in dietary fiber. This research indicates that value-added, nutritious snacks with reduced levels of flatulence factors and higher contents of dietary fiber can be fabricated successfully by extrusion processing of formulations based on lentil, dry pea or chickpea, and represent good alternatives to traditional cereal-based snacks. Also, the commercialization of value-added, pulse-based snacks would increase pulse consumption. Published by Elsevier Ltd. C1 [Berrios, J. De J.] ARS, USDA, WRRC, Albany, CA 94710 USA. [Morales, P.; Camara, M.; Sanchez-Mata, M. C.] Univ Complutense Madrid, Fac Farm, Dpto Nutr & Bromatol 2, Madrid, Spain. RP Berrios, JD (reprint author), ARS, USDA, WRRC, Albany, CA 94710 USA. EM jose.berrios@ars.usda.gov RI Camara, Montana/A-3520-2009; Sanchez-Mata, Maria de Cortes/G-8674-2015; Morales Gomez, Patricia/L-9267-2013 OI Camara, Montana/0000-0001-9232-0703; Sanchez-Mata, Maria de Cortes/0000-0002-7292-8663; Morales Gomez, Patricia/0000-0002-4963-2696 NR 35 TC 19 Z9 22 U1 10 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD MAR PY 2010 VL 43 IS 2 SI SI BP 531 EP 536 DI 10.1016/j.foodres.2009.09.035 PG 6 WC Food Science & Technology SC Food Science & Technology GA 563TW UT WOS:000275158400016 ER PT J AU Cao, JJ Gregoire, BR Sheng, XM Liuzzi, JP AF Cao, Jay J. Gregoire, Brian R. Sheng, Xiaoming Liuzzi, Juan P. TI Pinto bean hull extract supplementation favorably affects markers of bone metabolism and bone structure in mice SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE Bean hull extract; Bone; Micro CT; Resorption; Antioxidant ID PHASEOLUS-VULGARIS L.; ANTIOXIDANT ACTIVITY; HYDROGEN-PEROXIDE; DRY BEANS; AGE; DIFFERENTIATION; DAMAGE; OSTEOPROTEGERIN; MITOCHONDRIA; OSTEOPOROSIS AB Hulls from dry edible beans are rich in phenolic compounds recognized as possessing antioxidant activity. The aim of this study was to characterize antioxidant properties of bean hull extract (BHE) and to determine whether BHE supplementation (at 400 or 800 mg/kg for 3 months) affects serum biochemical markers and bone structure in 12-month-old male C57BL/6 mice. Mice supplemented with 800 mg BHE/kg had lower serum tartrate-resistant acid phosphatase and parathyroid hormone concentrations than those on control diet or supplemented with 400 mg BHE/kg. BHE supplementation caused slight decrease in oxidized glutathione concentration in blood (P = 0.07). Compared to the control group, BHE supplementation at 800 mg/kg for 3 months improved bone structural indices, bone mineral density and trabecular thickness in the third lumbar vertebra. These results suggest that BHE supplementation may have beneficial effects on bone health in mice by decreasing bone resorption. Published by Elsevier Ltd. C1 [Cao, Jay J.; Gregoire, Brian R.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Sheng, Xiaoming] Univ Utah, Salt Lake City, UT 84108 USA. [Liuzzi, Juan P.] Florida Int Univ, Miami, FL 33199 USA. RP Cao, JJ (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58202 USA. EM jay.Cao@ars.usda.gov; Brian.Gregoire@ars.usda.gov; Xiaoming.Sheng@utah.edu; Jliuzzi@fiu.edu FU USDA Agricultural Research Service program; Current Research Information System (CRIS) [5450-51000-039-00D]; Northarvest Bean Growers Association, Frazee, MN FX The USDA. Agricultural Research Service. Northern Plains Area, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. This work was supported by the USDA Agricultural Research Service program "Mineral Intakes for Optimal Bone Development and Health," Current Research Information System (CRIS) No. 5450-51000-039-00D. as part of the author's official duties.; Additional financial support was from the Northarvest Bean Growers Association, Frazee, MN. NR 49 TC 7 Z9 7 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 J9 FOOD RES INT JI Food Res. Int. PD MAR PY 2010 VL 43 IS 2 SI SI BP 560 EP 566 DI 10.1016/j.foodres.2009.07.031 PG 7 WC Food Science & Technology SC Food Science & Technology GA 563TW UT WOS:000275158400020 ER PT J AU Velazquez, G Candelario, HE Ramirez, JA Mangan, RL Loera-Gallardo, J Vazquez, M AF Velazquez, Gonzalo Candelario, Hugo E. Ramirez, Jose A. Mangan, Robert L. Loera-Gallardo, Jesus Vazquez, Manuel TI High Hydrostatic Pressure at Low Temperature as a Quarantine Treatment to Improve the Quality of Fruits SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID FLOUNDER ATHERESTHES-STOMIAS; MECHANICAL-PROPERTIES; PROTEINS; MANGO; STORAGE; POLYOLS; SUGARS; MUSCLE AB Application of high hydrostatic pressure has been proposed as an alternative quarantine process for the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae), one of the most important insects infesting mangoes, citrus fruits, and other fruits in Mexico and other Latin American countries. The present study was performed to determine the effect of high pressure treatments at 0 degrees C on the survivorship of eggs and larvae of this pest. The effect of time and pressure level at near-freezing temperatures on the egg hatch and survival of larvae is discussed. Eggs and larvae were pressurized at 25, 50, 75, 100, or 150 MPa for 0, 5, 10, or 20 min at 0 degrees C. Hatch was recorded for 1-, 2-, 3-, and 4-day-old pressurized eggs. For pressurized larvae (first, second, and third instars), percentage of survival was registered. Further, third instars were studied for their ability to pupate and develop to adulthood. The results showed that eggs had higher resistance than larvae to pressure. Larvae were not able to resist pressure treatments at 75 MPa for 20 min at 0 degrees C. Treatments at 150 MPa for 20 min at 0 degrees C were needed to destroy all eggs and larvae of A. ludens, indicating that this process might be useful as a quarantine method for infested fruits. However, more studies involving combination of high hydrostatic pressure at low temperature with precooling treatments are needed in order to decrease the pressure level to avoid fruit damage. C1 [Vazquez, Manuel] Univ Santiago de Compostela, Fac Vet Sci, Dept Analyt Chem, Lugo 27002, Spain. [Velazquez, Gonzalo; Candelario, Hugo E.; Ramirez, Jose A.] Univ Autonoma Tamaulipas, UAM Reynosa Aztlan, Dept Food Sci & Technol, Reynosa, Tamaulipas, Mexico. [Mangan, Robert L.] USDA ARS, Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Loera-Gallardo, Jesus] Campo Expt Rio Bravo, Rio Bravo, Tamaulipas, Spain. RP Vazquez, M (reprint author), Univ Santiago de Compostela, Fac Vet Sci, Dept Analyt Chem, Calle Carballo Calero S-N, Lugo 27002, Spain. EM manuel.vazquez@usc.es RI Vazquez, Manuel/K-7728-2012; Ramirez, Jose/M-6938-2013; Velazquez, Gonzalo/F-7701-2010 OI Vazquez, Manuel/0000-0002-0392-1724; Ramirez, Jose/0000-0001-7971-7044; Velazquez, Gonzalo/0000-0003-1901-9919 FU Fondo Mixto CONACYT; Tamaulipas state government [TAMPS-2003-C02-15] FX The authors are grateful to Fondo Mixto CONACYT (The National Council on Science and Technology) and Tamaulipas state government for financially supporting this work (Project TAMPS-2003-C02-15) and to CONACYT for the scholarship granted to author Hugo Ernesto Candelario. We also thank Jose Manuel Gutierrez Ruelas, M. Sc., Director of Fruit Flies, General Directorate of Plant Protection, Senasica, Sagarpa, and Dr. Pablo J. Montoya Gerardo, Assistant Director of Development Methods, Moscafrut Operational Program, for all the technical and scientific support. NR 28 TC 11 Z9 11 U1 0 U2 3 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAR PY 2010 VL 7 IS 3 BP 287 EP 292 DI 10.1089/fpd.2009.0399 PG 6 WC Food Science & Technology SC Food Science & Technology GA 562XL UT WOS:000275089100009 PM 19899960 ER PT J AU Davis, JC Castleberry, SB Kilgo, JC AF Davis, Justin C. Castleberry, Steven B. Kilgo, John C. TI Influence of coarse woody debris on herpetofaunal communities in upland pine stands of the southeastern Coastal Plain SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Amphibian; Coarse woody debris; Coastal plain; Herpetofauna; Longleaf; Pinus palustris; Reptile ID POPULATION ECOLOGY; EUMECES-LATICEPS; SPADEFOOT TOAD; WATER-LOSS; FOREST; TREE; SALAMANDERS; REPTILE; ECOSYSTEMS; AMBYSTOMA AB Coarse woody debris (CWD) is thought to benefit herpetofauna in a variety of ways including serving as feeding sites, providing a moist environment, and providing protection from temperature extremes. We investigated the importance of CWD to amphibian and reptile Communities in managed upland pine stands in the southeastern United States Coastal Plain during years 6 and 7 of a long-term Study. Using a randomized complete block design, I of the following treatments was assigned to 9.3-ha plots: removal (n = 3; all downed CWD >= 10 cm in diameter and >= 60 cm long removed), downed addition (n = 3: fivefold increase in volume of down CWD), snag (n = 3; 10-fold increase in volume of standing dead CWD), and control (n = 3; unmanipulated). Herpetofauna were captured seasonally using drift-fence pitfall trapping arrays within treatment plots. We compared abundance, diversity, and richness of anurans, salamanders, lizards, and snakes using analysis of covariance with topographic variables (slope, elevation, aspect, and distance to nearest stream) included as covariates. We captured 355 amphibians and 668 reptiles seasonally from January 2007 to August 2008. Abundance, species richness, and species diversity were similar among treatments for anurans, salamanders, and lizards. Snake abundance, species richness, and diversity were higher in removal than downed addition plots. Anuran abundance increased as distance to nearest stream decreased. The majority of species captured during this study are adept at burrowing into the sandy soils of the region. Lack of reliance on CWD may be the result of herpetofaunal adaptation to the longleaf pine (Pinus palustris) ecosystem that historically dominated the upland areas of the study area. (C) 2010 Elsevier B.V. All rights reserved. C1 [Davis, Justin C.; Castleberry, Steven B.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Kilgo, John C.] Forest Serv, USDA, So Res Stn, New Ellenton, SC 29809 USA. RP Castleberry, SB (reprint author), Univ Georgia, DB Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. EM scastle@warnell.uga.edu FU U. S. Department of Energy-Savannah River Operations Office through the U. S. Forest Service-Savannah River [DE-AI09-00SR22188] FX We acknowledge J. Blake, M. Dorcas, S. Loeb, and J. Laerm (deceased) who initiated this project. Mark Vukovich provided invaluable assistance on multiple facets of this project. We thank J. Linehan, A. McKee, D. Sollenberger, M. Goode, and M. Murphy for field assistance. Shannon Albeke provided guidance in GIS applications and analysis, and S. Ellis-Felege and R. J. Cooper assisted with statistical analysis. We thank the U. S. Department of Energy for allowing access to the Savannah River Site. Funding for this project was provided by the U. S. Department of Energy-Savannah River Operations Office through the U. S. Forest Service-Savannah River under Interagency Agreement DE-AI09-00SR22188. NR 55 TC 10 Z9 15 U1 7 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2010 VL 259 IS 6 BP 1111 EP 1117 DI 10.1016/j.foreco.2009.12.024 PG 7 WC Forestry SC Forestry GA 565SC UT WOS:000275313700007 ER PT J AU Gronewold, CA D'Amato, AW Palik, BJ AF Gronewold, Chris A. D'Amato, Anthony W. Palik, Brian J. TI The influence of cutting cycle and stocking level on the structure and composition of managed old-growth northern hardwoods SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Uneven-aged management; Northern hardwoods; Downed woody debris; Diameter distributions; Old growth; Stocking level; Single-tree selection ID COARSE WOODY DEBRIS; DIAMETER DISTRIBUTIONS; POPULATION-STRUCTURE; STAND STRUCTURE; UPPER MICHIGAN; CAVITY TREES; FORESTS; SELECTION; DYNAMICS; WISCONSIN AB Forest management approaches are increasingly being focused on restoring or retaining structurally complex old forest conditions. Evaluations of the long-term impacts of different management regimes on the maintenance and restoration of these conditions are critical for informing and refining current management approaches. To this end, we examined the long-term effects of differing intensities of single-tree selection harvest on stand structural attributes within northern hardwood forests in the Upper Peninsula of Michigan, USA. Stands in this study were classified as old growth at the onset of the experiment and were repeatedly treated and maintained at three residual stocking levels (11.5, 16.1, and 20.7 m(2) ha(-1) in trees greater than 24 cm DBH) with three different cutting cycle lengths (5, 10, and 15 years) over a 57-year period. Conditions within an adjacent, unmanaged old-growth system were used as a benchmark for comparing stand structural attributes, including abundance of downed woody debris (DWD), regeneration, and large diameter trees. Stand stocking level was the primary factor affecting the long-term trends in stand structure and composition observed among stands. In particular, stands maintained at a 11.5 m(2) ha(-1) stocking level had significantly lower amounts of DWD, snags, and large overstory trees compared to the 20.7 m(2) ha(-1) stocking level. Similarly, the highest stocking level treatment had the greatest abundance of Betula alleghaniensis relative to the other two stocking level treatments. In addition, long-term trends in diameter distribution forms indicated that the two highest stocking level treatments largely displayed increasing q and negative exponential curve forms over time, whereas the 11.5 m(2) ha(-1) stocking level was primarily characterized by concave distribution forms. Ordination analyses of stand structural attributes indicated that stands managed with the highest residual stocking levels contained stand structural characteristics approximating those found within the unmanaged, old-growth benchmark stand, whereas the lowest stocking level was much less complex. These results Suggest that single-tree selection systems that maintain high stand residual basal areas, a portion of which is made LIP of large diameter, below-grade, defective trees, could serve as a potential means to integrate or retain old-growth forest elements in managed stands. (C) 2010 Elsevier B.V. All rights reserved. C1 [Gronewold, Chris A.; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Palik, Brian J.] Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Gronewold, CA (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM gron0108@umn.edu FU Minnesota Agricultural Experiment Station; USDA Forest Service Northern Research Station FX The authors are grateful for the efforts of past USDA Forest Service scientists and technicians who established and maintained the Cutting Cycles and Stocking Levels Experiment, particularly Terry Strong, Gayne G. (Gus) Erdmann, Thomas Crow, and Christel Kern. In addition, Caleb Anderson assisted with data collection during the 2008 measurement season. Comments from an anonymous reviewer greatly improved this manuscript. Funding for this work was provided by the Minnesota Agricultural Experiment Station and USDA Forest Service Northern Research Station. NR 53 TC 21 Z9 22 U1 2 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2010 VL 259 IS 6 BP 1151 EP 1160 DI 10.1016/j.foreco.2010.01.001 PG 10 WC Forestry SC Forestry GA 565SC UT WOS:000275313700012 ER PT J AU Daniels, SE Kilgore, MA Jacobson, MG Greene, JL Straka, TJ AF Daniels, Steven E. Kilgore, Michael A. Jacobson, Michael G. Greene, John L. Straka, Thomas J. TI Examining the Compatibility between Forestry Incentive Programs in the US and the Practice of Sustainable Forest Management SO FORESTS LA English DT Article DE land management policies; non-industrial private forests; focus groups; sustainable forestry AB This research explores the intersection between the various federal and state forestry incentive programs and the adoption of sustainable forestry practices on non-industrial private forest (NIPF) lands in the US. The qualitative research reported here draws upon a series of eight focus groups of NIPF landowners (two each in Minnesota, Oregon, Pennsylvania, and South Carolina). Despite minor regional variations, the dominant theme that emerged is that these landowners' purchase and management decisions are motivated by the "trilogy" of forest continuity, benefit to the owner, and doing the "right thing." This trilogy is quite consistent with notions of sustainable forestry, but somewhat more at odds with the objectives of many financial incentive programs, as well as specific tactics such as third-party certification. A series of policy recommendations that emerge from this research is presented. C1 [Daniels, Steven E.] Utah State Univ, Dept Sociol Social Work & Anthropol, Logan, UT 84322 USA. [Kilgore, Michael A.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Jacobson, Michael G.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Greene, John L.] USDA Forest Serv, So Res Stn, Res Triangle Pk, NC 27709 USA. [Straka, Thomas J.] Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29634 USA. RP Straka, TJ (reprint author), Clemson Univ, Dept Forestry & Nat Resources, Box 340317, Clemson, SC 29634 USA. EM steve.daniels@usu.edu; mkilgore@umn.edu; mgj2@psu.edu; jgreene01@fs.fed.us; tstraka@clemson.edu NR 28 TC 4 Z9 4 U1 1 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAR PY 2010 VL 1 IS 1 BP 49 EP 64 DI 10.3390/f1010049 PG 16 WC Forestry SC Forestry GA V28BP UT WOS:000208656600004 ER PT J AU Rinaldi, M Li, RW Bannerman, DD Daniels, KM Evock-Clover, C Silva, MVB Paape, MJ Van Ryssen, B Burvenich, C Capuco, AV AF Rinaldi, Manuela Li, Robert W. Bannerman, Douglas D. Daniels, Kristy M. Evock-Clover, Christina Silva, Marcos V. B. Paape, Max J. Van Ryssen, Bernadette Burvenich, Christian Capuco, Anthony V. TI A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Dairy cow; Mastitis; Escherichia coli; Microarray; qRT-PCR; Network ID MAMMARY EPITHELIAL-CELLS; BACTERICIDAL/PERMEABILITY-INCREASING PROTEIN; LIPOPOLYSACCHARIDE-BINDING PROTEIN; COMPLEMENT FRAGMENT C5A; GRAM-NEGATIVE BACTERIA; ACUTE-PHASE RESPONSE; GROWTH-FACTOR-ALPHA; NF-KAPPA-B; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS AB Escherichia coli intramammary infection elicits localized and systemic responses, some of which have been characterized in mammary secretory tissue. Our objective was to characterize gene expression patterns that become activated in different regions of the mammary gland during the acute phase of experimentally induced E. coli mastitis. Tissues evaluated were from Furstenburg's rosette, teat cistern (TC), gland cistern (GC), and lobulo-alveolar (LA) regions of control and infected mammary glands, 12 and 24 h after bacterial (or control) infusions. The main networks activated by E. coli infection pertained to immune and inflammatory response, with marked induction of genes encoding proteins that function in chemotaxis and leukocyte activation and signaling. Genomic response at 12 h post-infection was greatest in tissues of the TC and GC. Only at 24 h post-infection did tissue from the LA region respond, at which time the response was the greatest of all regions. Similar genetic networks were impacted in all regions during early phases of intramammary infection, although regional differences throughout the gland were noted. Data support an important sentinel function for the teat, as these tissues responded rapidly and intensely, with production of cytokines and antimicrobial peptides. C1 [Rinaldi, Manuela; Li, Robert W.; Bannerman, Douglas D.; Daniels, Kristy M.; Evock-Clover, Christina; Silva, Marcos V. B.; Paape, Max J.; Capuco, Anthony V.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. [Silva, Marcos V. B.] Embrapa Dairy Cattle, BR-36038330 Juiz De Fora, MG, Brazil. [Van Ryssen, Bernadette] Univ Ghent, Dept Vet Med Imaging & Small Anim Orthoped, B-9820 Merelbeke, Belgium. [Burvenich, Christian] Univ Ghent, Dept Physiol & Biometr, B-9820 Merelbeke, Belgium. RP Capuco, AV (reprint author), ARS, Bovine Funct Genom Lab, USDA, Bldg 200,Rm 14,BARC E, Beltsville, MD 20705 USA. EM tony.capuco@ars.usda.gov OI Daniels, Kristy/0000-0002-1437-1457 FU United States Department of Agriculture, Agricultural Research Service [CRIS 1265-31000-083-00D]; Ghent University made possible through FWO Vlaanderen [G.0050.06N] FX The authors gratefully acknowledge the assistance of personnel of Research Animal Services, BARC. This research was funded by the United States Department of Agriculture, Agricultural Research Service, CRIS 1265-31000-083-00D, with participation by scientists at Ghent University made possible through FWO Vlaanderen, grant number G.0050.06N. NR 60 TC 29 Z9 31 U1 0 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD MAR PY 2010 VL 10 IS 1 BP 21 EP 38 DI 10.1007/s10142-009-0133-z PG 18 WC Genetics & Heredity SC Genetics & Heredity GA 567FP UT WOS:000275429100003 PM 19727872 ER PT J AU Connor, EE Kahl, S Elsasser, TH Parker, JS Li, RW Van Tassell, CP Baldwin, RL Barao, SM AF Connor, Erin E. Kahl, Stanislaw Elsasser, Theodore H. Parker, Joel S. Li, Robert W. Van Tassell, Curtis P. Baldwin, Ransom L. Barao, Scott M. TI Enhanced mitochondrial complex gene function and reduced liver size may mediate improved feed efficiency of beef cattle during compensatory growth SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Cattle; Feed efficiency; Liver; Microarray ID HIGHER OXIDATIVE STRESS; VISCERAL ORGAN MASS; DIVERGENT SELECTION; CALORIC RESTRICTION; PROTEIN EXPRESSION; METABOLIC-CHANGES; STEERS; MICROARRAY; BROILERS; RECEPTOR AB Growing ruminants under extended dietary restriction exhibit compensatory growth upon ad libitum feeding, which is associated with increased feed efficiency, lower basal energy requirements, and changes in circulating concentrations of metabolic hormones. To identify mechanisms contributing to these physiological changes, 8-month-old steers were fed either ad libitum (control; n = 6) or 60-70% of intake of control animals (feed-restricted; n = 6) for a period of 12 weeks. All steers were fed ad libitum for the remaining 8 weeks of experimentation (realimentation). Liver was biopsied at days -14, +1, and +14 relative to realimentation for gene expression analysis by microarray hybridization. During early realimentation, feed-restricted steers exhibited greater rates of gain and feed efficiency than controls and an increase in expression of genes functioning in cellular metabolism, cholesterol biosynthesis, oxidative phosphorylation, glycolysis, and gluconeogenesis. Gene expression changes during feed restriction were similar to those reported in mice, indicating similar effects of caloric restriction across species. Based on expression of genes involved in cell division and growth and upregulation of genes encoding mitochondrial complex proteins in early realimentation, it was concluded that reduced hepatic size and increased mitochondrial function may contribute to improved feed efficiency observed during compensatory growth. C1 [Connor, Erin E.; Kahl, Stanislaw; Elsasser, Theodore H.; Li, Robert W.; Van Tassell, Curtis P.; Baldwin, Ransom L.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Parker, Joel S.] Express Anal Inc, Durham, NC 27713 USA. [Barao, Scott M.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. RP Connor, EE (reprint author), ARS, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave,Bldg 200,Rm 4,BARC E, Beltsville, MD 20705 USA. EM erin.connor@ars.usda.gov OI Van Tassell, Curtis/0000-0002-8416-2087 FU US Department of Agriculture FX The authors thank Marsha Campbell and Dennis Hucht for their technical assistance and Roxane Macdonald, Ben Bache, Duane Taylor, George Bowman, Abraham Alignay, and the BARC Research Support Services staff for their assistance in conducting the animal portion of the study. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. Current affiliation of S. M. Barao is the Maryland Cattlemen's Association, 7566 Main St., Sykesville, MD 21784, USA. NR 61 TC 16 Z9 16 U1 2 U2 24 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD MAR PY 2010 VL 10 IS 1 BP 39 EP 51 DI 10.1007/s10142-009-0138-7 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 567FP UT WOS:000275429100004 PM 19777276 ER PT J AU Gu, YQ Wanjugi, H Coleman-Derr, D Kong, XY Anderson, OD AF Gu, Yong Qiang Wanjugi, Humphrey Coleman-Derr, Devin Kong, Xiuying Anderson, Olin D. TI Conserved globulin gene across eight grass genomes identify fundamental units of the loci encoding seed storage proteins SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Wheat; High molecular weight glutenin; Globulin; Duplication; Genome evolution; Domestication ID MOLECULAR-WEIGHT GLUTENIN; HEXAPLOID BREAD WHEAT; BARLEY D-HORDEIN; POLYPLOID WHEAT; EVOLUTIONARY HISTORY; ORTHOLOGOUS REGIONS; SEQUENCE-ANALYSIS; DUPLICATE GENES; HARDNESS LOCUS; MAIZE GENOME AB The wheat high molecular weight (HMW) glutenins are important seed storage proteins that determine bread-making quality in hexaploid wheat (Triticum aestivum). In this study, detailed comparative sequence analyses of large orthologous HMW glutenin genomic regions from eight grass species, representing a wide evolutionary history of grass genomes, reveal a number of lineage-specific sequence changes. These lineage-specific changes, which resulted in duplications, insertions, and deletions of genes, are the major forces disrupting gene colinearity among grass genomes. Our results indicate that the presence of the HMW glutenin gene in Triticeae genomes was caused by lineage-specific duplication of a globulin gene. This tandem duplication event is shared by Brachypodium and Triticeae genomes, but is absent in rice, maize, and sorghum, suggesting the duplication occurred after Brachypodium and Triticeae genomes diverged from the other grasses similar to 35 Ma ago. Aside from their physical location in tandem, the sequence similarity, expression pattern, and conserved cis-acting elements responsible for endosperm-specific expression further support the paralogous relationship between the HMW glutenin and globulin genes. While the duplicated copy in Brachypodium has apparently become nonfunctional, the duplicated copy in wheat has evolved to become the HMW glutenin gene by gaining a central prolamin repetitive domain. C1 [Gu, Yong Qiang; Wanjugi, Humphrey; Coleman-Derr, Devin; Anderson, Olin D.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Kong, Xiuying] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Crop Germplasm Resources & Utilizat,MOA, Beijing 10081, Peoples R China. RP Gu, YQ (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM yong.gu@ars.usda.gov FU NSF [DBI-0638558]; United State Department of Agriculture, Agriculture Research Service CRIS [532502100-000] FX The authors thank Jorge Dubcovsky for providing T. monococcum BAC clones, Sekou Health for assistance in the BAC sequencing, and Gerard R. Lazo for bioinformatics support. This work was supported in part by NSF Grant DBI-0638558 and by the United State Department of Agriculture, Agriculture Research Service CRIS 532502100-000 and 532502100-000. NR 52 TC 21 Z9 22 U1 3 U2 10 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD MAR PY 2010 VL 10 IS 1 BP 111 EP 122 DI 10.1007/s10142-009-0135-x PG 12 WC Genetics & Heredity SC Genetics & Heredity GA 567FP UT WOS:000275429100010 PM 19707805 ER PT J AU Kordonowy, LL McMurtry, JP Williams, TD AF Kordonowy, Lauren L. McMurtry, John P. Williams, Tony D. TI Variation in plasma leptin-like immunoreactivity in free-living European starlings (Sturnus vulgaris) SO GENERAL AND COMPARATIVE ENDOCRINOLOGY LA English DT Article DE Leptin-like immunoreactivity; European starling; Sturnus vulgaris; Reproduction; Seasonal variation ID DOG NYCTEREUTES-PROCYONOIDES; FOX ALOPEX-LAGOPUS; FOOD-INTAKE; SEASONAL-CHANGES; GROWTH-HORMONE; ADIPOSE-TISSUE; BODY-WEIGHT; MESSENGER-RNA; SERUM LEPTIN; OBESE GENE AB Leptin, a protein hormone secreted by fat cells, is best known for its role as an adiposity signal; however, leptin has diverse physiological roles ranging from regulation of feeding behavior and body weight, to effects on reproduction and immune function. Although leptin has been extensively studied in mammals, the identification and function of leptin in birds remains controversial, and studies have focused on captive or domesticated species. Here, we describe changes in plasma leptin-like immunoreactivity during the reproductive and non-reproductive seasons in free-living female European starlings (Sturnus vulgaris). Plasma leptin-like immunoreactivity was high during egg-laying (27.8 +/- 2.4 ng/mL) and clutch completion (23.8 +/- 1.6ng/mL), decreased during incubation (13.0 +/- 1.6ng/mL) and chick-rearing (12.0 +/- 1.3 ng/mL), but was elevated again in non-breeders in November (23.7 +/- 1.1 ng/mL). Although there was marked and consistent variation in total body mass and body composition with breeding stage and season in this population, plasma leptin-like immunoreactivity did not parallel changes in body mass or body composition. These data suggest that the strong positive relationship between plasma leptin-like immunoreactivity and body mass reported for captive birds and mammals does not hold for free-living birds. Rather, among free-living female European starlings, variation in plasma leptin-like immunoreactivity is associated with breeding stage or seasonal variation per se, and we discuss possible mechanisms underlying this variation, focusing on ovarian function and egg production. (C) 2009 Elsevier Inc. All rights reserved. C1 [Kordonowy, Lauren L.; Williams, Tony D.] Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada. [McMurtry, John P.] USDA, BARC E, Beltsville, MD 20705 USA. RP Kordonowy, LL (reprint author), Simon Fraser Univ, Dept Biol Sci, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada. EM lkordono@sfu.ca FU National Sciences and Engineering Research Council of Canada; Simon Fraser Graduate Fellowships FX We would like to acknowledge Dr. Sophie Bourgeon, Sarah Parker and Faezah Ali, who helped collect data during the 2007 breeding season and David Davis for the use of Davistead Dairy Farm as our field site. This research was funded through a National Sciences and Engineering Research Council of Canada Discovery grant to TDW, and Simon Fraser Graduate Fellowships to L.L.K. NR 49 TC 12 Z9 12 U1 1 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0016-6480 J9 GEN COMP ENDOCR JI Gen. Comp. Endocrinol. PD MAR 1 PY 2010 VL 166 IS 1 BP 47 EP 53 DI 10.1016/j.ygcen.2009.09.005 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 557MO UT WOS:000274673500007 PM 19796643 ER PT J AU Bassil, N Postman, JD AF Bassil, Nahla Postman, Joseph D. TI Identification of European and Asian pears using EST-SSRs from Pyrus SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Asian pear; European pear; Expressed sequence tags ( ESTs); Microsatellite markers; Pyrus; Simple sequence repeat (SSR) ID GENIC MICROSATELLITE MARKERS; GENETIC-RELATIONSHIPS; PYRIFOLIA NAKAI; RAPD MARKERS; CULTIVARS; L.; APPLE; CHIMERISM; DIVERSITY; FRAGARIA AB Ten EST-SSRs previously isolated from Pyrus were used to identify 81 P. communis, 13 P. pyrifolia and 20 P. ussuriensis or P. x bretschneideri accessions. Cross-transference of these EST-SSRs was high in these species. PYC-008 and PYC-004 were the least informative SSRs in each of the pear species and were monomorphic in P. pyrifolia while PYC-013, PYC-002 and PYC-009b were the most informative in all species. EST-SSRs were very valuable for identification of incorrectly identified accessions, failed grafts and sets of synonyms in each of the species. Unsuspected relationships were uncovered, including a parental relationship between 'Anjou' and 'Farmingdale', a clonal relationship between 'Berger' and 'Bartlett', and a very close relationship between 'Beurre Superfin' and 'Doyenne du Comice'. One SSR marker was different in one of three sports of 'Doyenne du Comice' ('Doyenne du Comice Crimson Gem') and in one of two sports of 'Anjou' ('Gebhard Red' red skin sport of 'Anjou'). UPGMA cluster analysis separated the pear accessions into a large European cluster and an Asian group mostly according to common ancestry, geographical origin or time of ripening. High cross-transference of EST-SSRs in Pyrus species is very valuable for germplasm management in such a highly diverse collection as found at the NCGR Pyrus genebank in Corvallis, OR. C1 [Bassil, Nahla; Postman, Joseph D.] ARS, USDA, Corvallis, OR 97333 USA. RP Bassil, N (reprint author), ARS, USDA, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM nahla.bassil@ars.usda.gov FU USDA-ARS CRIS [5358-21000-03300D]; USDA-ARS National Plant Germplasm System Evaluation FX We acknowledge Barbara Gilmore, Christine Neou-Anderson, and April Nyberg for technical assistance in microsatellite marker separation. Funding for this study was provided by the USDA-ARS CRIS 5358-21000-03300D and a USDA-ARS National Plant Germplasm System Evaluation Grant. NR 47 TC 26 Z9 32 U1 3 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD MAR PY 2010 VL 57 IS 3 BP 357 EP 370 DI 10.1007/s10722-009-9474-7 PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 567QC UT WOS:000275460700008 ER PT J AU Benatti, TR Valicente, FH Aggarwal, R Zhao, CY Walling, JG Chen, MS Cambron, SE Schemerhorn, BJ Stuart, JJ AF Benatti, Thiago R. Valicente, Fernando H. Aggarwal, Rajat Zhao, Chaoyang Walling, Jason G. Chen, Ming-Shun Cambron, Sue E. Schemerhorn, Brandon J. Stuart, Jeffrey J. TI A Neo-Sex Chromosome That Drives Postzygotic Sex Determination in the Hessian Fly (Mayetiola destructor) SO GENETICS LA English DT Article ID X-CHROMOSOME; Y-CHROMOSOME; DOSAGE COMPENSATION; EVOLUTIONARY STRATA; SCIARID FLIES; RECOMBINATION; DROSOPHILA; ELIMINATION; CONFLICT; GENES AB Two nonoverlapping autosomal inversions defined unusual neo-sex chromosomes in the Hessian fly (Mayetiola destructor). Like other neo-sex chromosomes, these were normally heterozygous, present only in one sex, and suppressed recombination around a sex-determining master switch. Their unusual properties originated from the anomalous Hessian fly sex determination system in which postzygotic chromosome elimination is used to establish the sex-determining karyotypes. This system permitted the evolution of a master switch (Chromosome maintenance, Cm) that acts maternally. All of the offspring of females that carry Cm-associated neo-sex chromosomes attain a female-determining somatic karyotype and develop as females. Thus, the chromosomes act as maternal effect neo-W's, or W-prime (W') chromosomes, where ZW' females mate with ZZ males to engender female-producing (ZW') and male-producing (ZZ) females in equal numbers. Genetic mapping and physical mapping identified the inversions. Their distribution was determined in nine populations. Experimental matings established the association of the inversions with Cm and measured their recombination suppression. The inversions are the functional equivalent of the sciarid X-prime chromosomes. We speculate that W' chromosomes exist in a variety of species that produce unisexual broods. C1 [Benatti, Thiago R.; Valicente, Fernando H.; Aggarwal, Rajat; Zhao, Chaoyang; Walling, Jason G.; Cambron, Sue E.; Schemerhorn, Brandon J.; Stuart, Jeffrey J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Cambron, Sue E.; Schemerhorn, Brandon J.] Purdue Univ, USDA ARS, W Lafayette, IN 47907 USA. RP Stuart, JJ (reprint author), Purdue Univ, Dept Entomol, 901 W State St, W Lafayette, IN 47907 USA. EM stuartjj@purdue.edu OI Zhao, Chaoyang/0000-0003-3378-3163; Schemerhorn, Brandon/0000-0003-0247-478X FU U.S. Department of Agriculture-National Research Initiative [2004-03099] FX We thank R. H. Shukle and P. Weintraub for providing Hessian fly populations, and we thank S. A. Jackson, V. Ferris, and two anonymous reviewers for comments that improved the manuscript. We gratefully acknowledge an award from the U.S. Department of Agriculture-National Research Initiative (2004-03099) that supported this work. NR 50 TC 13 Z9 13 U1 0 U2 6 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 J9 GENETICS JI Genetics PD MAR PY 2010 VL 184 IS 3 BP 769 EP U214 DI 10.1534/genetics.109.108589 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 650WZ UT WOS:000281886300014 PM 20026681 ER PT J AU Coles, ND McMullen, MD Balint-Kurti, PJ Pratt, RC Holland, JB AF Coles, Nathan D. McMullen, Michael D. Balint-Kurti, Peter J. Pratt, Richard C. Holland, James B. TI Genetic Control of Photoperiod Sensitivity in Maize Revealed by Joint Multiple Population Analysis SO GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; CORN GROWTH-RESPONSE; CONSTANS-LIKE GENE; FLOWERING-TIME; ARABIDOPSIS-THALIANA; TROPICAL MAIZE; CIRCADIAN-RHYTHMS; FLORAL INDUCTION; COMPLEX TRAITS; INBRED LINES AB Variation in maize for response to photoperiod is related to geographical adaptation in the species. Maize possesses homologs of many genes identified as regulators of flowering time in other species, but their relation to the natural variation for photoperiod response in maize is unknown. Candidate gene sequences were mapped in four populations created by crossing two temperate inbred lines to two photoperiod-sensitive tropical inbreds. Whole-genome scans were conducted by high-density genotyping of the populations, which were phenotyped over 3 years in both short- and long-day environments. Joint multiple population analysis identified genomic regions controlling photoperiod responses in flowering time, plant height, and total leaf number. Four key genome regions controlling photoperiod response across populations were identified, referred to as ZmPR1-4. Functional allelic differences within these regions among phenotypically similar founders suggest distinct evolutionary trajectories for photoperiod adaptation in maize. These regions encompass candidate genes CCA/LHY, CONZ1, CRY2, ELF4, GHD7, VGT1, HY1/SE5, TOC1/PRR7/PPD-1, PIF3, ZCN8, and ZCN19. C1 [Holland, James B.] N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Coles, Nathan D.; Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [McMullen, Michael D.] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [McMullen, Michael D.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Pratt, Richard C.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Holland, JB (reprint author), N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Box 7620, Raleigh, NC 27695 USA. EM Jim.Holland@ars.usda.gov OI Holland, James/0000-0002-4341-9675; Balint-Kurti, Peter/0000-0002-3916-194X FU Toulouse Genopole Midi-Pyrenees; USDA-Initiative for Future Agricultural and Food Systems multidisciplinary training grant [2001-52101-11507]; National Science Foundation [DBI-0321467]; USDA-Agricultural Research Service. FX We are grateful to Josie Bloom, Bob Baesman, David Rhyne, and Emily Silverman for technical assistance in SSR and candidate gene genotyping, and to Kate Guill for assistance with SNP genotyping. We thank Magen Eller, Brooke Peterson, Stella Salvo, Kathleen Starr, Scott Reed, and the crew of 27 farms for field assistance and help in phenotypic data collection; Arturo Garcia for data management assistance; Ed Buckler for candidate gene sequence information; and Janet Shurtleff and the staff of the North Carolina State University (NCSU) Phytotron. Sylvain Jasson generously provided technical assistance with MCQTL. GENOPLANTE MCQTL was generated within a GENOPLANTE program (MANGIN et al. 2007) and improved thanks to a grant from Toulouse Genopole Midi-Pyrenees. MCQTL analyses were performed using the resources of the NCSU High Performance Computing Center with the assistance of Gary Howell. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). This research was supported by a USDA-Initiative for Future Agricultural and Food Systems multidisciplinary training grant (award 2001-52101-11507), the National Science Foundation (DBI-0321467), and USDA-Agricultural Research Service. NR 91 TC 53 Z9 55 U1 2 U2 20 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 J9 GENETICS JI Genetics PD MAR PY 2010 VL 184 IS 3 BP 799 EP U301 DI 10.1534/genetics.109.110304 PG 64 WC Genetics & Heredity SC Genetics & Heredity GA 650WZ UT WOS:000281886300017 PM 20008571 ER PT J AU Chintamanani, S Hulbert, SH Johal, GS Balint-Kurti, PJ AF Chintamanani, Satya Hulbert, Scot H. Johal, Gurmukh S. Balint-Kurti, Peter J. TI Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization SO GENETICS LA English DT Article ID RUST RESISTANCE LOCUS; PR-LIKE PROTEIN; DISEASE RESISTANCE; INNATE IMMUNITY; MOLECULAR CHARACTERIZATION; NATURAL VARIATION; CELL-DEATH; CORN RUST; ZEA-MAYS; ARABIDOPSIS AB Potentially useful naturally occurring genetic variation is often difficult to identify as the effects of individual genes are subtle and difficult to observe. In this study, a novel genetic technique called Mutant-Assisted Gene Identification and Characterization is used to identify naturally occurring loci modulating the hypersensitive defense response (HR) in maize. Mutant-Assisted Gene Identification and Characterization facilitates the identification of naturally occurring alleles underlying phenotypic variation from diverse germplasm, using a mutant phenotype as a "reporter." In this study the reporter phenotype was caused by a partially dominant autoactive disease resistance gene, Rp1-D21, which caused HR lesions to form spontaneously all over the plant. Here it is demonstrated that the Rp1-D21 phenotype is profoundly affected by genetic background. By crossing the Rp1-D21 gene into the IBM mapping population, it was possible to map and identify Hrml1 on chromosome 10, a locus responsible for modulating the HR phenotype conferred by Rp1-D21. Other loci with smaller effects were identified on chromosomes 1 and 9. These results demonstrate that Mutant-Assisted Gene Identification and Characterization is a viable approach for identifying naturally occurring useful genetic variation. C1 [Balint-Kurti, Peter J.] N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Chintamanani, Satya; Johal, Gurmukh S.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Hulbert, Scot H.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Balint-Kurti, PJ (reprint author), N Carolina State Univ, USDA ARS, Plant Sci Res Unit, 3418 Gardner Hall, Raleigh, NC 27695 USA. EM peter.balint-kurti@ars.usda.gov OI Balint-Kurti, Peter/0000-0002-3916-194X FU USDA-ARS, Purdue University; National Science Foundation (NSF) [0822495] FX We are grateful to the following for assistance and valuable advice: Cliff Weil, Randy Wisser, Rebecca Nelson, Donna Stephens, Kristen Kump, Rahul Dhawan, and Hugh Young. This work was funded by USDA-ARS, Purdue University, and a National Science Foundation (NSF) Grant 0822495. NR 48 TC 20 Z9 22 U1 2 U2 9 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 1943-2631 J9 GENETICS JI Genetics PD MAR PY 2010 VL 184 IS 3 BP 813 EP 825 DI 10.1534/genetics.109.111880 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 650WZ UT WOS:000281886300018 PM 20176981 ER PT J AU VanRaden, PM Sullivan, PG AF VanRaden, Paul M. Sullivan, Peter G. TI International genomic evaluation methods for dairy cattle SO GENETICS SELECTION EVOLUTION LA English DT Article ID GENETIC EVALUATIONS; MULTIPLE-TRAIT; ANIMAL-MODEL; INFORMATION; PREDICTIONS; RELIABILITY; SELECTION AB Background: Genomic evaluations are rapidly replacing traditional evaluation systems used for dairy cattle selection. Higher reliabilities from larger genotype files promote cooperation across country borders. Genomic information can be exchanged across countries using simple conversion equations, by modifying multi-trait across-country evaluation (MACE) to account for correlated residuals originating from the use of foreign evaluations, or by multi-trait analysis of genotypes for countries that use the same reference animals. Methods: Traditional MACE assumes independent residuals because each daughter is measured in only one country. Genomic MACE could account for residual correlations using daughter equivalents from genomic data as a fraction of the total in each country and proportions of bulls shared. MACE methods developed to combine separate within-country genomic evaluations were compared to direct, multi-country analysis of combined genotypes using simulated genomic and phenotypic data for 8,193 bulls in nine countries. Results: Reliabilities for young bulls were much higher for across-country than within-country genomic evaluations as measured by squared correlations of estimated with true breeding values. Gains in reliability from genomic MACE were similar to those of multi-trait evaluation of genotypes but required less computation. Sharing of reference genotypes among countries created large residual correlations, especially for young bulls, that are accounted for in genomic MACE. Conclusions: International genomic evaluations can be computed either by modifying MACE to account for residual correlations across countries or by multi-trait evaluation of combined genotype files. The gains in reliability justify the increased computation but require more cooperation than in previous breeding programs. C1 [VanRaden, Paul M.] USDA, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [Sullivan, Peter G.] Canadian Dairy Network, Guelph, ON N1K 1E5, Canada. RP VanRaden, PM (reprint author), USDA, Anim Improvement Programs Lab, Bldg 5 BARC W, Beltsville, MD 20705 USA. EM Paul.VanRaden@ars.usda.gov NR 20 TC 29 Z9 32 U1 0 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X J9 GENET SEL EVOL JI Genet. Sel. Evol. PD MAR 1 PY 2010 VL 42 AR 7 DI 10.1186/1297-9686-42-7 PG 9 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA 573JI UT WOS:000275908900001 PM 20193071 ER PT J AU Carroll, C Dunk, JR Moilanen, A AF Carroll, Carlos Dunk, Jeffrey R. Moilanen, Atte TI Optimizing resiliency of reserve networks to climate change: multispecies conservation planning in the Pacific Northwest, USA SO GLOBAL CHANGE BIOLOGY LA English DT Article DE bioclimatic niche; connectivity; conservation prioritization; global change; spatial optimization; species distribution modeling; spotted owl; Zonation ID NORTHERN SPOTTED OWL; PSEUDO-ABSENCE DATA; SPECIES DISTRIBUTIONS; FOREST PLAN; DISTRIBUTION MODELS; SAMPLE-SIZE; OLD-GROWTH; SELECTION; PERFORMANCE; MADAGASCAR AB The effectiveness of a system of reserves may be compromised under climate change as species' habitat shifts to nonreserved areas, a problem that may be compounded when well-studied vertebrate species are used as conservation umbrellas for other taxa. The Northwest Forest Plan was among the first efforts to integrate conservation of wide-ranging focal species and localized endemics into regional conservation planning. We evaluated how effectively the plan's focal species, the Northern Spotted Owl, acts as an umbrella for localized species under current and projected future climates and how the regional system of reserves can be made more resilient to climate change. We used the program maxent to develop distribution models integrating climate data with vegetation variables for the owl and 130 localized species. We used the program zonation to identify a system of areas that efficiently captures habitat for both the owl and localized species and prioritizes refugial areas of climatic and topographic heterogeneity where current and future habitat for dispersal-limited species is in proximity. We projected future species' distributions based on an ensemble of contrasting climate models, and incorporating uncertainty between alternate climate projections into the prioritization process. Reserve solutions based on the owl overlap areas of high localized-species richness but poorly capture core areas of localized species' distribution. Congruence between priority areas across taxa increases when refugial areas are prioritized. Although core-area selection strategies can potentially increase the conservation value and resilience of regional reserve systems, they accentuate contrasts in priority areas between species and over time and should be combined with a broadened taxonomic scope and increased attention to potential effects of climate change. Our results suggest that systems of fixed reserves designed for resilience can increase the likelihood of retaining the biological diversity of forest ecosystems under climate change. C1 [Carroll, Carlos] Klamath Ctr Conservat Res, Orleans, CA 95556 USA. [Dunk, Jeffrey R.] Humboldt State Univ, Dept Environm & Nat Resource Sci, Arcata, CA 95521 USA. [Dunk, Jeffrey R.] US Forest Serv, Redwood Sci Lab, Pacific SW Res Stn, USDA, Arcata, CA 95521 USA. [Moilanen, Atte] Univ Helsinki, Dept Biol & Environm Sci, FI-00014 Helsinki, Finland. RP Carroll, C (reprint author), Klamath Ctr Conservat Res, Orleans, CA 95556 USA. EM carlos@klamathconservation.org RI Moilanen, Atte/A-5005-2011 FU Academy of Finland FX We thank the US Forest Service, Bureau of Land Management, and California Department of Fish and Game for providing owl or localized species survey data, and E. Salathe for providing future climate projections. M. Visser, S. Phillips, H. Kujala, and two anonymous reviewers provided helpful comments. A. M. thanks the Academy of Finland centre of excellence program 2006-2011 for support. NR 45 TC 84 Z9 85 U1 9 U2 106 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2010 VL 16 IS 3 BP 891 EP 904 DI 10.1111/j.1365-2486.2009.01965.x PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 554EY UT WOS:000274419500001 ER PT J AU Rittenhouse, CD Pidgeon, AM Albright, TP Culbert, PD Clayton, MK Flather, CH Huang, CQ Masek, JG Radeloff, VC AF Rittenhouse, Chadwick D. Pidgeon, Anna M. Albright, Thomas P. Culbert, Patrick D. Clayton, Murray K. Flather, Curtis H. Huang, Chengquan Masek, Jeffrey G. Radeloff, Volker C. TI Avifauna response to hurricanes: regional changes in community similarity SO GLOBAL CHANGE BIOLOGY LA English DT Article DE abundance; biodiversity; birds; community similarity; hurricane; North American Breeding Bird Survey; richness; United States ID STATES VIRGIN-ISLANDS; BIRD POPULATIONS; CLIMATE-CHANGE; UNITED-STATES; MIGRATORY BIRDS; SOUTHERN MISSISSIPPI; FOREST FRAGMENTATION; SPECIES RICHNESS; PUERTO-RICO; HUGO AB Global climate models predict increases in the frequency and intensity of extreme climatic events such as hurricanes, which may abruptly alter ecological processes in forests and thus affect avian diversity. Developing appropriate conservation measures necessitates identifying patterns of avifauna response to hurricanes. We sought to answer two questions: (1) does avian diversity, measured as community similarity, abundance, and species richness, change in areas affected by hurricane compared with unaffected areas, and (2) what factors are associated with the change(s) in avian diversity? We used North American Breeding Bird Survey data, hurricane track information, and a time series of Landsat images in a repeated measures framework to answer these questions. Our results show a decrease in community similarity in the first posthurricane breeding season for all species as a group, and for species that nest in the midstory and canopy. We also found significant effects of hurricanes on abundance for species that breed in urban and woodland habitats, but not on the richness of any guild. In total, hurricanes produced regional changes in community similarity largely without significant loss of richness or overall avian abundance. We identified several potential mechanisms for these changes in avian diversity, including hurricane-induced changes in forest habitat and the use of refugia by birds displaced from hurricane-damaged forests. The prospect of increasing frequency and intensity of hurricanes is not likely to invoke a conservation crisis for birds provided we maintain sufficient forest habitat so that avifauna can respond to hurricanes by shifting to areas of suitable habitat. C1 [Rittenhouse, Chadwick D.; Pidgeon, Anna M.; Albright, Thomas P.; Culbert, Patrick D.; Radeloff, Volker C.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Clayton, Murray K.] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA. [Flather, Curtis H.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Huang, Chengquan] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Masek, Jeffrey G.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. RP Rittenhouse, CD (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM cdrittenhous@wisc.edu RI Masek, Jeffrey/D-7673-2012; Rittenhouse, Chadwick/G-7169-2012; Radeloff, Volker/B-6124-2016; Flather, Curtis/G-3577-2012; OI Radeloff, Volker/0000-0001-9004-221X; Flather, Curtis/0000-0002-0623-3126; Huang, Chengquan/0000-0003-0055-9798 FU NASA FX We thank the thousands of volunteers who have collected BBS data and made studies such as this one possible. We thank David Helmers and Nicholas Keuler for providing technical and statistical support. We thank Tracy Rittenhouse, Frederic Beaudry, Eric Wood, and two anonymous reviewers for comments that improved this paper. We gratefully acknowledge funding from the NASA Biodiversity Program and the NASA Interdisciplinary Sciences Program. NR 70 TC 17 Z9 17 U1 6 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2010 VL 16 IS 3 BP 905 EP 917 DI 10.1111/j.1365-2486.2009.02101.x PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 554EY UT WOS:000274419500002 ER PT J AU Srygley, RB Dudley, R Oliveira, EG Aizprua, R Pelaez, NZ Riveros, AJ AF Srygley, Robert B. Dudley, Robert Oliveira, Evandro G. Aizprua, Rafael Pelaez, Nicole Z. Riveros, Andre J. TI El Nino and dry season rainfall influence hostplant phenology and an annual butterfly migration from Neotropical wet to dry forests SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climate change; El Nino; ENSO; insect flight; insect migration; migratory behavior; resource limitation; tropical rainforest ID WIND DRIFT COMPENSATION; CLIMATE-CHANGE; HERBIVORE OUTBREAK; FLUCTUATIONS; LEPIDOPTERA; ENSO; CONSEQUENCES; ECOSYSTEMS; STRATEGIES; CALIFORNIA AB We censused butterflies flying across the Panama Canal at Barro Colorado Island (BCI) for 16 years and butterfly hostplants for 8 years to address the question: What environmental factors influence the timing and magnitude of migrating Aphrissa statira butterflies? The peak migration date was earlier when the wet season began earlier and when soil moisture content in the dry season preceding the migration was higher. The peak migration date was also positively associated with peak leaf flushing of one hostplant (Callichlamys latifolia) but not another (Xylophragma seemannianum). The quantity of migrants was correlated with the El Nino Southern Oscillation, which influenced April soil moisture on BCI and total rainfall in the dry season. Both hostplant species responded to El Nino with greater leaf flushing, and the number of adults deriving from or laying eggs on those new leaves was greatest during El Nino years. The year 1993 was exceptional in that the number of butterflies migrating was lower than predicted by the El Nino event, yet the dry season was unusually wet for an El Nino year as well. Thus, dry season rainfall appears to be a primary driver of larval food production and population outbreaks for A. statira. Understanding how global climate cycles and local weather influence tropical insect migrations improves the predictability of ecological effects of climate change. C1 [Srygley, Robert B.; Dudley, Robert] Smithsonian Trop Res Inst, Balboa, Panama. [Dudley, Robert] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Oliveira, Evandro G.] Ctr Univ UNA, Fac Ciencias Biol & Saude, BR-30180100 Belo Horizonte, MG, Brazil. [Aizprua, Rafael] Univ Panama, Dept Bot, Panama City, Panama. [Pelaez, Nicole Z.] Univ Los Andes, Dept Ciencias Biol, Bogota, Colombia. [Riveros, Andre J.] Univ Nacl Colombia, Dept Biol, Bogota, Colombia. RP Srygley, RB (reprint author), ARS, USDA, NPARL, 1500 N Cent Ave, Sidney, MT 59270 USA. EM robert.srygley@ars.usda.gov FU Smithsonian Institution; National Geographic Society Committee for Research and Exploration FX A Senior Postdoctoral Fellowship to R. B. S. from the Smithsonian Institution and grants from the National Geographic Society Committee for Research and Exploration supported the research. The Autoridad Nacional del Ambiente (ANAM) granted permission to conduct the research in Panama. Hydrological and radiation data were provided by the Terrestrial-Environmental Science Program of the STRI. Season begin and end dates were provided by the Meteorological and Hydrological Branch of the Panama Canal Authority (ACP). For presentational purposes, data were modified from their original form. We thank the meteorologists and their parent institutions for their long-term commitment to these essential programs. We also thank S. Paton for numerous discussions and assistance with BCI climate and its effects, and Joe Wright and Egbert Leigh for commenting on the manuscript. NR 39 TC 19 Z9 19 U1 3 U2 44 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2010 VL 16 IS 3 BP 936 EP 945 DI 10.1111/j.1365-2486.2009.01986.x PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 554EY UT WOS:000274419500004 ER PT J AU Polley, HW Emmerich, W Bradford, JA Sims, PL Johnson, DA Saliendra, NZ Svejcar, T Angell, R Frank, AB Phillips, RL Snyder, KA Morgan, JA AF Polley, H. Wayne Emmerich, William Bradford, James A. Sims, Phillip L. Johnson, Douglas A. Saliendra, Nicanor Z. Svejcar, Tony Angell, Raymond Frank, Albert B. Phillips, Rebecca L. Snyder, Keirith A. Morgan, Jack A. TI Physiological and environmental regulation of interannual variability in CO2 exchange on rangelands in the western United States SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climatic variability; desert; functional change; grassland; leaf area; light-use efficiency; net ecosystem exchange of CO2; precipitation; respiration; shrubland ID NET ECOSYSTEM EXCHANGE; CARBON-DIOXIDE FLUXES; WATER-VAPOR; LONG-TERM; USE EFFICIENCY; BOWEN-RATIO; RESPIRATION; BALANCE; GRASSLAND; PRAIRIE AB For most ecosystems, net ecosystem exchange of CO2 (NEE) varies within and among years in response to environmental change. We analyzed measurements of CO2 exchange from eight native rangeland ecosystems in the western United States (58 site-years of data) in order to determine the contributions of photosynthetic and respiratory (physiological) components of CO2 exchange to environmentally caused variation in NEE. Rangelands included Great Plains grasslands, desert shrubland, desert grasslands, and sagebrush steppe. We predicted that (1) week-to-week change in NEE and among-year variation in the response of NEE to temperature, net radiation, and other environmental drivers would be better explained by change in maximum rates of ecosystem photosynthesis (A(max)) than by change in apparent light-use efficiency (alpha) or ecosystem respiration at 10 degrees C (R-10) and (2) among-year variation in the responses of NEE, A(max), and alpha to environmental drivers would be explained by changes in leaf area index (LAI). As predicted, NEE was better correlated with A(max) than alpha or R-10 for six of the eight rangelands. Week-to-week variation in NEE and physiological parameters correlated mainly with time-lagged indices of precipitation and water-related environmental variables, like potential evapotranspiration, for desert sites and with net radiation and temperature for Great Plains grasslands. For most rangelands, the response of NEE to a given change in temperature, net radiation, or evaporative demand differed among years because the response of photosynthetic parameters (A(max), alpha) to environmental drivers differed among years. Differences in photosynthetic responses were not explained by variation in LAI alone. A better understanding of controls on canopy photosynthesis will be required to predict variation in NEE of rangeland ecosystems. C1 [Polley, H. Wayne] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Emmerich, William] ARS, USDA, Tucson, AZ 85719 USA. [Bradford, James A.; Sims, Phillip L.] ARS, USDA, Woodward, OK 73801 USA. [Johnson, Douglas A.] ARS, USDA, Logan, UT 84322 USA. [Saliendra, Nicanor Z.] US Forest Serv, Rhinelander, WI 54501 USA. [Svejcar, Tony; Angell, Raymond] ARS, USDA, Burns, OR 97720 USA. [Frank, Albert B.; Phillips, Rebecca L.] ARS, USDA, Mandan, ND 58554 USA. [Snyder, Keirith A.] ARS, USDA, Reno, NV 89512 USA. [Morgan, Jack A.] ARS, USDA, Ft Collins, CO 80526 USA. RP Polley, HW (reprint author), ARS, USDA, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM wayne.polley@ars.usda.gov RI Phillips, Rebecca/G-6175-2015 OI Phillips, Rebecca/0000-0003-3881-9065 NR 31 TC 24 Z9 24 U1 1 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2010 VL 16 IS 3 BP 990 EP 1002 DI 10.1111/j.1365-2486.2009.01966.x PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 554EY UT WOS:000274419500008 ER PT J AU Jensen, KB Waldron, BL Peel, MD Robins, JG AF Jensen, K. B. Waldron, B. L. Peel, M. D. Robins, J. G. TI Nutritive value of herbage of five semi-irrigated pasture species across an irrigation gradient SO GRASS AND FORAGE SCIENCE LA English DT Article DE smooth bromegrass; prairiegrass; hybrid-bromegrass; creeping foxtail; RS wheatgrass; irrigation; nutritive value ID FORAGE QUALITY; PERENNIAL RYEGRASS; WATER-STRESS; DIGESTIBILITY; ORCHARDGRASS; ALFALFA; GRASSES; GROWTH; YIELD AB As water resources become limiting, the need to produce stable amounts of herbage with a high nutritive value increases. An understanding of how levels of irrigation affect crude protein (CP) and neutral-detergent fibre (NDF) concentrations and, in vitro true digestibility (IVTD), is critical in the management of pastures. Smooth bromegrass (Bromus inermis Leyss.), prairiegrass (Bromus willdenowii Kunth), hybrid-bromegrass [meadow (Bromus riparius Rehm.) x smooth bromegrass], creeping foxtail (Alopecurus arundinaceus Poir.) and RS wheatgrass (Elymus hoffmannii Jensen & Asay) were established under a line-source irrigation system to evaluate the effect of five water levels and three harvest dates on concentrations of CP and NDF, and values of IVTD. With CP concentration of herbage, there were positive linear significant relationships with decreasing water levels for all species. Rankings for mean CP concentrations across water levels and harvest dates were smooth bromegrass > RS wheatgrass > hybrid-bromegrass > creeping foxtail > prairiegrass. No consistent relationships with IVTD across water levels were detected among the species. Averaged across water levels, harvest dates and years, smooth bromegrass, hybrid-bromegrass and creeping foxtail had significantly higher IVTD values than RS wheatgrass and prairiegrass. Rankings of mean NDF concentrations across water levels and harvest dates combined over years were prairiegrass > RS wheatgrass > hybrid-bromegrass > creeping foxtail > smooth bromegrass. Herbage of smooth bromegrass had higher IVTD values and lower NDF concentrations than hybrid-bromegrass, prairiegrass, creeping foxtail and RS-hybrid at all water levels. C1 [Jensen, K. B.; Waldron, B. L.; Peel, M. D.; Robins, J. G.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. RP Jensen, KB (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. EM kevin.jensen@ars.usda.gov NR 27 TC 3 Z9 3 U1 0 U2 13 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0142-5242 J9 GRASS FORAGE SCI JI Grass Forage Sci. PD MAR PY 2010 VL 65 IS 1 BP 92 EP 101 DI 10.1111/j.1365-2494.2009.00723.x PG 10 WC Agronomy SC Agriculture GA 557ZU UT WOS:000274710400012 ER PT J AU Goldman, J Springer, T AF Goldman, Jason Springer, Tim TI Population dynamics of greenbug biotypes "E" and "F" on Texas bluegrass SO GRASSLAND SCIENCE LA English DT Article DE Biotype; greenbug aphid; interspecific hybrid; Texas bluegrass ID APHIDIDAE BIOTYPES; HOMOPTERA; RESISTANT AB Cool-season perennial forage grasses are known to serve as alternate hosts of the greenbug, Schizaphis graminum (Rondani) in the southern Great Plains of the USA. Texas bluegrass (Poa arachnifera Torr.) is a cool-season perennial forage grass indigenous to this region and because of its persistence under grazing is being bred for improved forage production. An experiment was conducted to determine the population dynamics of greenbug biotype "E", the predominant biotype found in this region and biotype "F" which is known to infest bluegrass species. Twenty-five Texas bluegrass genotypes, three Kentucky bluegrass (Poa pratensis L.) genotypes, a Canada bluegrass (Poa compressa L.) genotype, two Texas x Kentucky bluegrass hybrids, and a Texas x Canada bluegrass hybrid were screened for their abilities to support greenbug population growth. Bluegrass species and genotypes varied significantly in their ability to support greenbug biotypes E and F. On the basis of higher aphid numbers, biotype E had a higher population growth rate as compared with biotype F on Texas bluegrass. Some Texas bluegrass genotypes were not included in both biotype trials due to lack of availability of uniform plant material for inoculation. Nine of 25 Texas bluegrass genotypes did not support biotype E greenbug population growth. Similarly, 13 of 22 Texas bluegrass genotypes did not support biotype F population growth. Both Canada and Kentucky bluegrass supported high populations of biotype F and interspecific hybrids varied in their host suitability to both biotypes. C1 [Goldman, Jason; Springer, Tim] ARS, USDA, So Plains Range Res Stn, Woodward, OK 73801 USA. RP Goldman, J (reprint author), ARS, USDA, So Plains Range Res Stn, 2000 18th St, Woodward, OK 73801 USA. EM Jason.Goldman@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1744-6961 J9 GRASSL SCI JI Grassl. Sci. PD MAR PY 2010 VL 56 IS 1 BP 26 EP 30 DI 10.1111/j.1744-697X.2009.00169.x PG 5 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA 582PY UT WOS:000276611300005 ER PT J AU Qin, B Polansky, MM Anderson, RA AF Qin, B. Polansky, M. M. Anderson, R. A. TI Cinnamon Extract Regulates Plasma Levels of Adipose-derived Factors and Expression of Multiple Genes Related to Carbohydrate Metabolism and Lipogenesis in Adipose Tissue of Fructose-fed Rats SO HORMONE AND METABOLIC RESEARCH LA English DT Article DE cinnamon extract; adiponectin; FABP4; RBP4; adipose tissue ID ACID-BINDING PROTEIN; INTESTINAL INSULIN-RESISTANCE; FED HAMSTER MODEL; BIOLOGICAL-ACTIVITY; ADIPONECTIN LEVELS; 3T3-L1 ADIPOCYTES; APOLIPOPROTEIN-E; OBESITY; ATHEROSCLEROSIS; OVERPRODUCTION AB We reported earlier that dietary cinnamon extract (CE) improves systemic insulin sensitivity and dyslipidemia by enhancing insulin signaling. In the present study, we have examined the effects of CE on several biomarkers including plasma levels of adipose-derived adipokines, and the potential molecular mechanisms of CE in epididymal adipose tissue ( EAT). In Wistar rats fed a high-fructose diet (HFD) to induce insulin resistance, supplementation with a CE (Cinnulin PF (R), 50 mg/kg daily) for 8 weeks reduced blood glucose, plasma insulin, triglycerides, total cholesterol, chylomicron-apoB48, VLDL-apoB100, and soluble CD36. CE also inhibited plasma retinol binding protein 4 (RBP4) and fatty acid binding protein 4 (FABP4) levels. CE-induced increases in plasma adiponectin were not significant. CE did not affect food intake, bodyweight, and EAT weight. In EAT, there were increases in the insulin receptor (Ir) and Ir substrate 2 (Irs2) mRNA, but CE-induced increases in mRNA expression of Irs1, phosphoinositide-3-kinase, Akt1, glucose transporters 1 and 4, and glycogen synthase 1 expression and decreased trends in mRNA expression of glycogen synthase kinase 3 beta were not statistically significant. CE also enhanced the mRNA levels of adipoQ, and inhibited sterol regulatory element binding protein-1c mRNA levels. mRNA and protein levels of fatty acid synthase and FABP4 were inhibited by CE and RBP4, and CD36 protein levels were also decreased by CE. These results suggest that CE effectively ameliorates circulating levels of adipokines partially mediated via regulation of the expression of multiple genes involved in insulin sensitivity and lipogenesis in the EAT. C1 [Qin, B.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. Integr Nutraceut Int, S Spring Hill, TN USA. RP Qin, B (reprint author), ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Bldg 307C,Room 215,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Bolin.Qin@ars.usda.gov; Richard.Anderson@ars.usda.gov FU USDA Cooperative Research and Development Agreement (CRADA) FX Dr. R.A. Anderson has a USDA Cooperative Research and Development Agreement (CRADA) with Integrity Nutraceuticals International. Dr. B. Qin is a visiting scientist, working at the Diet, Genomics, and Immunology Laboratory, USDA/ARS/BHNRC, and is also employed by Integrity. NR 48 TC 20 Z9 21 U1 2 U2 6 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0018-5043 J9 HORM METAB RES JI Horm. Metab. Res. PD MAR PY 2010 VL 42 IS 3 BP 187 EP 193 DI 10.1055/s-0029-1242746 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 568CY UT WOS:000275500600006 PM 19937569 ER PT J AU Hayes, RJ Wu, BM Pryor, BM Chitrampalam, P Subbarao, KV AF Hayes, Ryan J. Wu, Bo Ming Pryor, Barry M. Chitrampalam, Periasamy Subbarao, Krishna V. TI Assessment of Resistance in Lettuce (Lactuca sativa L.) to Mycelial and Ascospore Infection by Sclerotinia minor Jagger and S. sclerotiorum (Lib.) de Bary SO HORTSCIENCE LA English DT Article DE genetic variation; breeding; cultivars; disease resistance; regression; bolting ID SCREENING LETTUCE; DROP; IDENTIFICATION; EPIDEMIOLOGY; TEMPERATURE; LINES AB Lettuce drop caused by Sclerotinia spp. is an economically important disease of lettuce (Lactuca sativa L.), and cultivars with resistance to mycelial infection by Sclerotinia sclerotiorum (Lib.) de Bary and S. minor Jagger as well as to S. sclerotiorum ascospores are needed. Assessing resistance in field experiments can be complicated by fast bolting or small stature lettuce lines that may escape rather than resist the pathogens. Therefore, methods to select resistant lines from morphologically variable populations are needed. We used S. sclerotiorum and S. minor-infested field experiments, regression analysis, field experiments with artificially high plant densities, and S. sclerotiorum ascospore inoculations to identify lettuce lines with resistance to both pathogens. Three replicated experiments in S. sclerotiorum-infested fields were conducted in Yuma, AZ, and three replicated experiments in a S. minor-infested field were conducted in Salinas, CA, using diverse populations of iceberg, romaine, leaf, butterhead, Latin, oilseed lettuce, and wild relatives of lettuces; and genetic variation for the incidence of lettuce drop from mycelial infections was identified. In two S. minor field experiments, a quadratic regression model was developed that related rapid bolting with reduced lettuce drop. Regression residuals were calculated, and eight cultivars or Pis had negative residuals in two independent field experiments, indicating higher resistance than predicted by their rate of bolting. Eruption, a small-statured Latin cultivar, had significantly lower disease levels than susceptible cultivars in experiments with high plant densities, indicating that its small size did not facilitate disease escape. Ascospore inoculations confirmed resistance in 'Eruption' and L. virosa SAL012, whereas the oilseed lettuce PI 251246 may have partial resistance to infection. These lines will likely be useful for development of Sclerotinia spp.-resistant lettuce cultivars. C1 [Hayes, Ryan J.] USDA ARS, Crop Improvement & Protect Unit, Salinas, CA 93905 USA. [Wu, Bo Ming] Oregon State Univ, Dept Bot & Plant Pathol, Cent Oregon Agr Res Ctr, Madras, OR 97741 USA. [Pryor, Barry M.] Univ Arizona, Div Plant Pathol & Microbiol, Tucson, AZ 85721 USA. [Chitrampalam, Periasamy; Subbarao, Krishna V.] US Agr Res Stn, Salinas, CA 93905 USA. [Chitrampalam, Periasamy; Subbarao, Krishna V.] Univ Calif, Dept Plant Pathol, Davis, CA USA. RP Hayes, RJ (reprint author), USDA ARS, Crop Improvement & Protect Unit, 1636 E Alisal St, Salinas, CA 93905 USA. EM Ryan.Hayes@ars.usda.gov OI Subbarao, Krishna/0000-0002-2075-1835 FU California Leafy Greens Research; Arizona Iceberg Lettuce Research Council FX This research was supported by the California Leafy Greens Research Program and the Arizona Iceberg Lettuce Research Council. NR 30 TC 12 Z9 13 U1 1 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 333 EP 341 PG 9 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000001 ER PT J AU Russo, VM Perkins-Veazie, P AF Russo, Vincent M. Perkins-Veazie, P. TI Yield and Nutrient Content of Bell Pepper Pods from Plants Developed from Seedlings Inoculated, or Not, with Microorganisms SO HORTSCIENCE LA English DT Article DE arbuscular mycorrhizae; bacteria; carotenoid; chlorophyll; conventional; fungus; organic; vitamin C ID GROWTH-PROMOTING RHIZOBACTERIA; TRANSPLANT PRODUCTION; MYCORRHIZAL FUNGI; AMENDMENT; QUALITY; ROOTS; SOIL; RESISTANCE; FERTILIZER; MUSKMELON AB The effectiveness of microorganisms applied in production of vegetable transplants has had mixed results. Bell pepper (Capsicum autumn, L.) transplants were grown in a greenhouse using organic methods and the organic-certified potting mix was inoculated, or not, with beneficial bacteria or arbuscular mycorrhizal (AM) fungi, or both. Other transplants were grown in the same greenhouse with a conventional potting mix, which was not inoculated. Transplants were established in the field at various planting dates in a Bernow or Stigler soil and grown using conventional or organic methods. Pod yield and nutrient contents were determined. Yields of organically grown plants were similar to those of conventionally grown plants when both were grown on the Bernow soil. When grown on both soil types, yield was higher for the Bernow than for the Stigler soil. Treatment had little effect on pod mineral content. Chlorophylls, total carotenoid, and vitamin C contents of pods from plants grown on the Stigler soil were generally lower than those from plants grown on the Bernow soil. Average pod fresh weight for plants developed from seedlings inoculated with beneficial bacteria or AM fungi was greater than that from plants developed from conventionally grown seedlings. Inoculation did not improve fresh pod weights over that from plants developed from organically grown, but not inoculated, seedlings. Amending potting mix with the microorganisms tested did not provide extraordinary benefit or detriment for use in production of bell pepper. C1 [Russo, Vincent M.; Perkins-Veazie, P.] USDA ARS, S Cent Agr Res Lab, Lane, OK 74555 USA. RP Russo, VM (reprint author), USDA ARS, S Cent Agr Res Lab, 911 Highway 3W, Lane, OK 74555 USA. EM vincent.russo@ars.usda.gov NR 42 TC 13 Z9 15 U1 3 U2 16 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 352 EP 358 PG 7 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000004 ER PT J AU Baietto, M Wilson, AD AF Baietto, Manuela Wilson, A. Dan TI Relative In Vitro Wood Decay Resistance of Sapwood from Landscape Trees of Southern Temperate Regions SO HORTSCIENCE LA English DT Article DE trunk-rot fungi; root-rot fungi; tree selection; urban forestry ID WHITE-ROT FUNGI; CONDUCTIVE POLYMER ANALYSIS; GANODERMA-AUSTRALE; LIGNIN DEGRADATION; ARMILLARIA-OSTOYAE; EUCALYPTUS-NITENS; IDENTIFICATION; PATTERNS; DAMAGE; HOST AB The development of wood decay caused by 12 major root-rot and trunk-rot fungi was investigated in vitro with sapwood extracted from nine ornamental and landscape hardwood and conifer species native to southern temperate regions of North America, Europe, and the lower Mississippi Delta. Wood decay rates based on dry weight loss for 108 host tree wood decay fungi combinations were compared at 21 degrees C over 1-year and 2-year incubation periods in the absence of tree-resistance mechanisms. Strains of Armillaria mellea, Ganoderma lucidum, and Heterobasidion annosum exhibited the highest decay potential in most tree species tested. The order of fungi causing the greatest decay varied over time as a result of temporal changes in decay-rate curves. Relative wood durability or resistance to decay generally was greater in gymnosperm than in angiosperm wood types. Quercus nuttallii, Fraxinus pennsylvanica, and Quercus lyrata sustained the highest levels of decay by all fungi. Northern white cedar (Thuja occidentalis) sapwood was most resistant to decay by all rot-fungi tested, sustaining only limited weight loss after 1 and 2 years of decay, although sapwood of Mutts taeda, Liquidambar styraciflua, and Platanus occidentalis had relatively low levels of decay after 2 years. These results in combination with data from portable decay-detection devices provide useful information for the management of tree breakages or failures resulting from wood decay fungi in hazardous landscape trees. Some potential landscaping applications for tree evaluations, risk assessments, and selections for tree-replacement plantings are discussed. C1 [Baietto, Manuela] Univ Milan, Dipartimento Prod Vegetale, I-20133 Milan, MI, Italy. [Wilson, A. Dan] US Forest Serv, USDA, SRS, So Hardwoods Lab, Stoneville, MS 38776 USA. RP Baietto, M (reprint author), Univ Milan, Dipartimento Prod Vegetale, Via Celoria 2, I-20133 Milan, MI, Italy. EM manuela.baietto@unimi.it FU Comune di Milano; Settore Tecnico Arredo Urbano e Verde; Demetra Societa Cooperativa Sociale ONLUS; Besana Brianza, MI, Italy FX This research was funded by Comune di Milano, Settore Tecnico Arredo Urbano e Verde, and from Demetra Societa Cooperativa Sociale ONLUS, Besana Brianza, MI, Italy. NR 65 TC 3 Z9 4 U1 4 U2 15 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 401 EP 408 PG 8 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000012 ER PT J AU Finn, CE Strik, BC Yorgey, B Qian, M Martin, RR Peterson, M AF Finn, Chad E. Strik, Bernadine C. Yorgey, Brian Qian, Michael Martin, Robert R. Peterson, Mary TI 'Wild Treasure' Thornless Trailing Blackberry SO HORTSCIENCE LA English DT Editorial Material DE Rubus ursinus; germplasm; processing; small fruit; fruit breeding C1 [Finn, Chad E.; Martin, Robert R.; Peterson, Mary] ARS, USDA, Hort Crops Res Lab, NW Ctr Small Fruit Res, Corvallis, OR 97330 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yorgey, Brian; Qian, Michael] Oregon State Univ, Dept Food Sci, Corvallis, OR 97331 USA. RP Finn, CE (reprint author), ARS, USDA, Hort Crops Res Lab, NW Ctr Small Fruit Res, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM Chad.Finn@ARS.USDA.GOV NR 5 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 434 EP 436 PG 3 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000019 ER PT J AU Finn, CE Strik, BC Yorgey, B Martin, RR Stahler, MM AF Finn, Chad E. Strik, Bernadine C. Yorgey, Brian Martin, Robert R. Stahler, Margaret M. TI 'Newberry' Trailing Blackberry SO HORTSCIENCE LA English DT Editorial Material DE processing; small fruit; fruit breeding; 'Boysen' C1 [Finn, Chad E.; Martin, Robert R.] ARS, USDA, Hort Crops Res Lab, NW Ctr Small Fruit Res, Corvallis, OR 97330 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yorgey, Brian] Oregon State Univ, Dept Food Sci, Corvallis, OR 97331 USA. RP Finn, CE (reprint author), ARS, USDA, Hort Crops Res Lab, NW Ctr Small Fruit Res, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM Chad.Finn@ARS.USDA.GOV NR 9 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 437 EP 440 PG 4 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000020 ER PT J AU Ledbetter, CA AF Ledbetter, Craig A. TI 'Bolaroja' and 'Primarosa': Two New Midseason Apricots for the Fresh Market SO HORTSCIENCE LA English DT Editorial Material DE fruit breeding; fruit quality; Prunus armeniaca L.; self-incompatibility C1 ARS, USDA, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. RP Ledbetter, CA (reprint author), ARS, USDA, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Craig.Ledbetter@ars.usda.gov NR 4 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 441 EP 442 PG 2 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000021 ER PT J AU Bowman, KD AF Bowman, Kim D. TI Longevity of Radiofrequency Identification Device Microchips in Citrus Trees SO HORTSCIENCE LA English DT Article DE rootstock improvement; Citrus sinensis; sweet orange; Carrizo citrange ID TRANSPONDERS AB Long-term identification of individual plants in the field is an important part of many types of research. In a previous report, we described methods for using implanted radiofrequency identification device (RFID) microchips to tag citrus trees for field research. This report provides an update on the RFID technology for use in plants, the effect of implanted chips on long-term plant growth, and survival of the microchips over time. The microchips were found to have no significant effect on plant health and growth, and most microchips continued to work well through the first 6 years after implantation. Implanted RFID microchips appear useful for long-term tagging of citrus and other woody plant species. C1 ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Bowman, KD (reprint author), ARS, US Hort Res Lab, USDA, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM kim.bowman@ars.uda.gov NR 7 TC 13 Z9 14 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 451 EP 452 PG 2 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000025 ER PT J AU Kopsell, DA Sams, CE Deyton, DE Abney, KR Kopsell, DE Robertson, L AF Kopsell, Dean A. Sams, Carl E. Deyton, Dennis E. Abney, Kristin R. Kopsell, David E. Robertson, Larry TI Characterization of Nutritionally Important Carotenoids in Bunching Onion SO HORTSCIENCE LA English DT Article ID BRASSICA-OLERACEA; GENETIC-VARIATION; MACULAR PIGMENT; BETA-CAROTENE; HIGHER-PLANTS; SULFUR; LUTEIN; ABSORPTION; VEGETABLES; NITROGEN AB Members of the Allium genus are consumed for their culinary flavor attributes, but also contain antioxidant and anticarcinogenic phytochemicals. Bunching onions (Allium fistulosum L.) are commonly used in Asian cuisine, in which both leaves and pseudostems are consumed. Carotenoids and chlorophylls are important classes of phytochemicals gaining attention for their health attributes. The goal of our study was to characterize carotenoids and chlorophylls and identify possible genetic and environmental influences on carotenoid concentrations among A. fistulosum accessions. Twelve USDA-ARS accessions were field grown in Knoxville, TN, and Geneva, NV, during the summer of 2007. After harvest, carotenoid and chlorophyll pigments were evaluated in leaf and pseudostem tissues using high-performance liquid chromatography. We were able to identify the presence of antheraxanthin, beta-carotene, chlorophyll a and b, lutein, neoxanthin, and violaxanthin in leaf tissues; however, pigments were not found in pseudostem tissues. Carotenoid and chlorophyll concentrations did not differ among accessions or between locations. It is possible that accessions evaluated in this study were a narrow genetic base or were selected based on flavor attributes and not leaf tissue pigmentation. C1 [Kopsell, Dean A.; Sams, Carl E.; Deyton, Dennis E.; Abney, Kristin R.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Kopsell, David E.] Illinois State Univ, Dept Agr, Normal, IL 61790 USA. [Robertson, Larry] USDA ARS, NE Reg Plant Introduct Stn, Plant Genet Resource Unit, Geneva, NY 14456 USA. RP Kopsell, DA (reprint author), Univ Tennessee, Dept Plant Sci, 252 Ellington Plant Sci,2431 Joa Johnson Dr, Knoxville, TN 37996 USA. EM dkopsell@utk.edu RI Barickman, Thomas/I-6993-2012 FU University of Tennessee Institute of Agriculture FX This research was made possible through support from the University of Tennessee Institute of Agriculture. NR 28 TC 3 Z9 4 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD MAR PY 2010 VL 45 IS 3 BP 463 EP 465 PG 3 WC Horticulture SC Agriculture GA 582JT UT WOS:000276594000029 ER PT J AU Medhurst, RB Wipfli, MS Binckley, C Polivka, K Hessburg, PF Salter, RB AF Medhurst, R. Bruce Wipfli, Mark S. Binckley, Chris Polivka, Karl Hessburg, Paul F. Salter, R. Brion TI Headwater streams and forest management: Does ecoregional context influence logging effects on benthic communities? SO HYDROBIOLOGIA LA English DT Article DE Headwater streams; Benthic macroinvertebrates; Ecoregion; Logging; Cascade Range ID ALDER ALNUS-RUBRA; SOUTHEASTERN ALASKA; SPATIAL-PATTERNS; INVERTEBRATE; DETRITUS; USA; MACROINVERTEBRATES; CLASSIFICATION; ASSEMBLAGES; WASHINGTON AB Effects of forest management on stream communities have been widely documented, but the role that climate plays in the disturbance outcomes is not understood. In order to determine whether the effect of disturbance from forest management on headwater stream communities varies by climate, we evaluated benthic macroinvertebrate communities in 24 headwater streams that differed in forest management (logged-roaded vs. unlogged-unroaded, hereafter logged and unlogged) within two ecological sub-regions (wet versus dry) within the eastern Cascade Range, Washington, USA. In both ecoregions, total macroinvertebrate density was highest at logged sites (P = 0.001) with gathering-collectors and shredders dominating. Total taxonomic richness and diversity did not differ between ecoregions or forest management types. Shredder densities were positively correlated with total deciduous and Sitka alder (Alnus sinuata) riparian cover. Further, differences in shredder density between logged and unlogged sites were greater in the wet ecoregion (logging x ecoregion interaction; P = 0.006) suggesting that differences in post-logging forest succession between ecoregions were responsible for differences in shredder abundance. Headwater stream benthic community structure was influenced by logging and regional differences in climate. Future development of ecoregional classification models at the subbasin scale, and use of functional metrics in addition to structural metrics, may allow for more accurate assessments of anthropogenic disturbances in mountainous regions where mosaics of localized differences in climate are common. C1 [Medhurst, R. Bruce] Univ Alaska, Alaska Cooperat Fish & Wildlife Res Unit, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. [Wipfli, Mark S.; Binckley, Chris] Univ Alaska, Alaska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Polivka, Karl; Hessburg, Paul F.; Salter, R. Brion] US Forest Serv, Pacific NW Res Stn, USDA, Wenatchee, WA 98801 USA. RP Medhurst, RB (reprint author), POB 1363, Mammoth Lakes, CA 93546 USA. EM ebbnflow@yahoo.com FU BonnevilleBonneville Power Administration [2003-017]; Mazamas foundation FX This work was part of the Integrated Status and Effectiveness Monitoring Project funded by Bonneville Power Administration (Project number 2003-017). We thank Chris Jordan, Michael Ward, and Pamela Nelle for administrative support with project funding. Thanks to Josh Kill, Andy McCracken, Jake Layman, Bessie Green, Melissa Smith, and Galina Popova, for help with field work and laboratory processing. We would like to thank David Herbst, Arny Blanchard, Pete Bisson, and Robert Denehy for early manuscript reviews. Additional funding from the Mazamas foundation helped make this work possible. The use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by the U. S. Government. NR 43 TC 4 Z9 5 U1 1 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 J9 HYDROBIOLOGIA JI Hydrobiologia PD MAR PY 2010 VL 641 IS 1 BP 71 EP 83 DI 10.1007/s10750-009-0058-5 PG 13 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA 552TX UT WOS:000274317600007 ER PT J AU Bolten, JD Crow, WT Zhan, XW Jackson, TJ Reynolds, CA AF Bolten, John D. Crow, Wade T. Zhan, Xiwu Jackson, Thomas J. Reynolds, Curt A. TI Evaluating the Utility of Remotely Sensed Soil Moisture Retrievals for Operational Agricultural Drought Monitoring SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Agriculture; data assimilation; remote sensing; soil moisture ID LAND-SURFACE MODELS; DATA ASSIMILATION; AMSR-E; VEGETATION; PRODUCTS AB Soil moisture is a fundamental data source used by the United States Department of Agriculture (USDA) International Production Assessment Division (IPAD) to monitor crop growth stage and condition and subsequently, globally forecast agricultural yields. Currently, the USDA IPAD estimates surface and root-zone soil moisture using a two-layer modified Palmer soil moisture model forced by global precipitation and temperature measurements. However, this approach suffers from well-known errors arising from uncertainty in model forcing data and highly simplified model physics. Here, we attempt to correct for these errors by designing and applying an Ensemble Kalman filter (EnKF) data assimilation system to integrate surface soil moisture retrievals from the NASA Advanced Microwave Scanning Radiometer (AMSR-E) into the USDA modified Palmer soil moisture model. An assessment of soil moisture analysis products produced from this assimilation has been completed for a five-year (2002 to 2007) period over the North American continent between 23 degrees N-50 degrees N and 128 degrees W-65 degrees W. In particular, a data denial experimental approach is utilized to isolate the added utility of integrating remotely sensed soil moisture by comparing EnKF soil moisture results obtained using (relatively) low-quality precipitation products obtained from real-time satellite imagery to baseline Palmer model runs forced with higher quality rainfall. An analysis of root-zone anomalies for each model simulation suggests that the assimilation of AMSR-E surface soil moisture retrievals can add significant value to USDA root-zone predictions derived from real-time satellite precipitation products. C1 [Bolten, John D.] NASA, Goddard Space Flight Ctr, Hydrol Sci Branch, Greenbelt, MD 20771 USA. [Crow, Wade T.; Jackson, Thomas J.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Zhan, Xiwu] NOAA, Ctr Satellite Applicat & Res, Natl Environm Satellite Data & Informat Serv, Camp Springs, MD 20746 USA. [Reynolds, Curt A.] USDA ARS, Int Prod Assessment Div, Off Global Anal, Washington, DC 20002 USA. RP Bolten, JD (reprint author), NASA, Goddard Space Flight Ctr, Hydrol Sci Branch, Greenbelt, MD 20771 USA. EM john.bolten@nasa.gov; wade.crow@ars.usda.gov; xiwu.zhan@noaa.gov; tom.jackson@ars.usda.gov; curt.reynolds@fas.usda.gov RI Zhan, Xiwu/F-5487-2010; Bolten, John/F-9006-2012 FU NASA [NNS06AA051] FX This work was supported by funding from NASA's Applied Sciences Program and grant NNS06AA051 entitled "Integrating NASA's Global Soil Moisture Remote Sensing and Modeling Data into the USDA's Global Crop Production Decision Support System". NR 29 TC 77 Z9 81 U1 8 U2 64 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD MAR PY 2010 VL 3 IS 1 BP 57 EP 66 DI 10.1109/JSTARS.2009.2037163 PG 10 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA 561TM UT WOS:000275001800006 ER PT J AU Narvekar, PS Heygster, G Jackson, TJ Bindlish, R Macelloni, G Notholt, J AF Narvekar, Parag S. Heygster, Georg Jackson, Thomas J. Bindlish, Rajat Macelloni, Giovanni Notholt, Justus TI Passive Polarimetric Microwave Signatures Observed Over Antarctica SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Ice; microwave radiometry; polarimetry; snow ID GREENLAND ICE-SHEET; SNOW ACCUMULATION; SURFACE-PROPERTIES; WINDSAT; RADIOMETER; SCATTEROMETER; CALIBRATION; VARIABILITY; BACKSCATTER; EMISSION AB WindSat fully polarimetric passive microwave observations, expressed in the form of the Stokes vector, were analyzed over the Antarctic ice sheet. The vertically and horizontally polarized brightness temperatures (first two Stokes components) from WindSat are shown to be consistent with previous studies. Azimuthal modulations in the third and fourth Stokes components were analyzed and related to surface topography, roughness, and snow morphology. A second harmonic sine function of the azimuth angle was used to estimate the orientation angle of snow features, such as topographic slope and sastrugi. The results show good agreement with the orientations derived in a previous study using scatterometer data at similar frequencies. Seasonal variability in the third and fourth Stokes components is discussed. A consistent pattern of response emerged for 10.7 GHz. Under winter conditions, the large contribution of multiple volume scattering causes a high and regionally varying 10.7-GHz fourth Stokes signal. Under summer conditions, surface scattering dominates and results in a high 10.7-GHz third Stokes signal. The third and fourth Stokes observations at 37 GHz were found to correspond to the smaller penetration depth at this higher frequency, resulting in a low difference between the amplitudes of summer and winter. The study demonstrates the potential of the spaceborne fully polarimetric passive microwave radiometers in monitoring the thermal and morphological properties of large ice sheets. C1 [Narvekar, Parag S.; Heygster, Georg; Notholt, Justus] Univ Bremen, Inst Environm Phys, D-28334 Bremen, Germany. [Jackson, Thomas J.; Bindlish, Rajat] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Bindlish, Rajat] Sci Syst & Applicat Inc, Lanham, MD 20706 USA. [Macelloni, Giovanni] CNR, Ist Fis Applicata Nello Carrara, I-50019 Sesto Fiorentino, Italy. RP Narvekar, PS (reprint author), CUNY, New York, NY 10075 USA. EM narvekar@iup.physik.uni-bremen.de; heygster@uni-bremen.de; tjackson@hydrolab.arsusda.gov; rajat.bindlish@ars.usda.gov; g.macelloni@ifac.cnr.it; jnotholt@iup.physik.uni-bremen.de RI Macelloni, Giovanni /B-7518-2015; Notholt, Justus/P-4520-2016 OI Notholt, Justus/0000-0002-3324-885X NR 32 TC 7 Z9 8 U1 0 U2 6 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAR PY 2010 VL 48 IS 3 BP 1059 EP 1075 DI 10.1109/TGRS.2009.2032295 PN 1 PG 17 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 559BL UT WOS:000274794600007 ER PT J AU Artlip, T Wisniewski, M Norelli, J AF Artlip, Timothy Wisniewski, Michael Norelli, John TI Constitutive Expression of a Peach AP2/ERF Transcription Factor in Apple Confers Short-Day Cessation of Growth SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Artlip, Timothy; Wisniewski, Michael; Norelli, John] USDA ARS, Kearneysville, WV 25425 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S95 EP S96 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500210 ER PT J AU Beasley, R Pijut, P AF Beasley, Rochelle Pijut, Paula TI Regeneration of Plants from Black Ash (Fraxinus nigra Marsh) Hypocotyls SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Beasley, Rochelle] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Pijut, Paula] USDA, Forest Serv, No Res Stn, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S98 EP S98 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500217 ER PT J AU Chaweewan, Y Anderson, JV Horvath, D Taylor, N Fauquet, C AF Chaweewan, Yeetoh Anderson, James, V Horvath, David Taylor, Nigel Fauquet, Claude TI Identifying the Gene Expression Profile of Tuberization in Cassava (Manihot esculenta Crantz) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Chaweewan, Yeetoh; Taylor, Nigel; Fauquet, Claude] Donald Danforth Plant Sci Ctr, ILTAB, St Louis, MO USA. [Anderson, James, V; Horvath, David] USDA ARS, Plant Sci Res Unit, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S207 EP S208 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500468 ER PT J AU Garg, A Owens, T Setter, T Miller, W Kim, JK Kochian, L Wu, R AF Garg, Ajay Owens, Thomas Setter, Tim Miller, William Kim, Ju-Kon Kochian, Leon Wu, Ray TI Trehalose Accumulation in Rice, Maize, and Wheat Plants Confers High Tolerance Levels to Different Abiotic Stresses SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Garg, Ajay] Myongji Univ, Dept Mol Biol & Genet, Kyonggi Do 449728, South Korea. [Garg, Ajay; Owens, Thomas] Myongji Univ, Dept Plant Biol, Kyonggi Do 449728, South Korea. [Setter, Tim] Myongji Univ, Dept Crop & Soil Sci, Kyonggi Do 449728, South Korea. [Miller, William] Myongji Univ, Dept Hort, Kyonggi Do 449728, South Korea. [Kim, Ju-Kon] Myongji Univ, Dept Biol Sci, Kyonggi Do 449728, South Korea. [Kochian, Leon] Cornell Univ, Robert Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Wu, Ray] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA. [Garg, Ajay] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14850 USA. [Garg, Ajay] Cornell Univ, Dept Plant Biol, Ithaca, NY 14850 USA. NR 0 TC 0 Z9 0 U1 2 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S204 EP S204 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500459 ER PT J AU Goodman, C Stanley, D Beeman, R Park, YS AF Goodman, Cynthia Stanley, David Beeman, Richard Park, Yoonseong TI A Cell Culture Derived from the Red Flour Beetle, Tribolium castaneum SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Goodman, Cynthia; Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Beeman, Richard] ARS, USDA, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. [Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S117 EP S117 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500260 ER PT J AU Grusak, M Tang, GW Yin, SA Wang, Y Qin, JA Dolnikowski, G Russell, R AF Grusak, Michael Tang Guangwen Yin Shi-an Wang Yin Qin Jian Dolnikowski, Gregory Russell, Robert TI Golden Rice Development, Nutritional Assessment, and Potential for Alleviating Vitamin A Deficiency SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Grusak, Michael] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Tang Guangwen; Qin Jian; Dolnikowski, Gregory; Russell, Robert] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Yin Shi-an] Natl Inst Nutr & Food Safety, Beijing, Peoples R China. [Wang Yin] Zhejiang Acad Med Sci, Zhejiang, Peoples R China. NR 0 TC 0 Z9 0 U1 7 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S30 EP S31 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500068 ER PT J AU Hoekenga, O Gustin, J Flint-Garcia, S Rocheford, T Settles, M McMullen, M Baxter, I AF Hoekenga, Owen Gustin, Jeff Flint-Garcia, Sherry Rocheford, Torbert Settles, Mark McMullen, Mike Baxter, Ivan TI Ionomics of the Maize Nested Association Mapping Panel SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Hoekenga, Owen] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. [Gustin, Jeff; Settles, Mark] Univ Florida, Gainesville, FL 32611 USA. [Flint-Garcia, Sherry; McMullen, Mike] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [Rocheford, Torbert] Purdue Univ, W Lafayette, IN 47907 USA. [Baxter, Ivan] USDA ARS, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S9 EP S9 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500019 ER PT J AU Huang, YH AF Huang Yinghua TI Genomic Approaches to Plant Defense Research and Crop Pest Management SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Huang Yinghua] ARS, USDA, Plant Sci Res Lab, Stillwater, OK 74075 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S125 EP S125 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500278 ER PT J AU Jenderek, M Ambruzs, B Andre, C Laufman, J Ellis, D AF Jenderek, Maria Ambruzs, Barbara Andre, Christina Laufman, Julie Ellis, David TI In Vitro Maintenance and Cryostorage of Plant Variety Protection Voucher Germplasm of Solanum tuberosum Cultivars SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Jenderek, Maria; Ambruzs, Barbara; Andre, Christina; Ellis, David] USDA ARS, NCGRP, Ft Collins, CO 80521 USA. [Laufman, Julie] USDA ARS, Natl Forest Serv, Ft Collins, CO 80521 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S127 EP S127 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500283 ER PT J AU Jenderek, M Ambruzs, B Postman, J Ellis, D AF Jenderek, Maria Ambruzs, Barbara Postman, Joseph Ellis, David TI Dormant Bud Cryopreservation for Germplasm Conservation SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Jenderek, Maria; Ambruzs, Barbara; Ellis, David] USDA ARS, NCGRP, Ft Collins, CO 80521 USA. [Postman, Joseph] USDA ARS, NCGR, Corvallis, OR 97333 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S35 EP S36 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500079 ER PT J AU Kraemer, F Hake, S Pauly, M AF Kraemer, Florian Hake, Sarah Pauly, Markus TI Identifying New Genes Influencing the Cell Wall of Grasses Using a Forward Genetic Approach SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pauly, Markus] Univ Calif Berkeley, Energy Biosci Inst, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Hake, Sarah] USDA ARS, Plant Gene Express Ctr, Albany, CA 94710 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S134 EP S135 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500299 ER PT J AU Kumar, P Stier, G Jiang, CZ Reid, M AF Kumar, Pushpendra Stier, Genevieve Jiang Cai-Zhong Reid, Michael TI Interruption of New Proteins Synthesis Delays Flower Senescence A Molecular Approach SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kumar, Pushpendra; Stier, Genevieve; Reid, Michael] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Jiang Cai-Zhong] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S135 EP S135 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500301 ER PT J AU Lee, JD Shannon, J Bilyeu, K Pham, A AF Lee, Jeong-Dong Shannon, J. Bilyeu, Kristin Pham, Anh TI Molecular Genetics of Soybeans with the High Oleic Acid Trait SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Lee, Jeong-Dong] Kyungpook Natl Univ, Div Plant Biosci, Taegu 702701, South Korea. [Shannon, J.] Univ Missouri, Delta Res Ctr, Div Plant Sci, Portageville, MO 63873 USA. [Bilyeu, Kristin] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Pham, Anh] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S203 EP S204 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500458 ER PT J AU Maiti, I Chatterjee, A Das, N Raha, S Babbitt, R Huang, QW Zaitlin, D Fan, ZM Kurt, W Charles, L Kong, Q AF Maiti, Indu Chatterjee, Aparajita Das, Narayan Raha, Sumita Babbitt, Ruth Huang Qingwei Zaitlin, David Fan Zhanmin Kurt, Wagscha Charles, Lee Kong, Que TI Expression of Engineered Xylan-degrading Chimeric Enzymes for Bioenergy and Biofuel Applications SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Maiti, Indu; Babbitt, Ruth; Huang Qingwei; Zaitlin, David; Fan Zhanmin; Kong, Que] Univ Kentucky, Lexington, KY 40546 USA. [Chatterjee, Aparajita] Boston Univ, Boston, MA 02215 USA. [Das, Narayan] Scottish Church Coll, Kolkata, W Bengal, India. [Raha, Sumita] Northwestern Univ, Evanston, IL 60208 USA. [Kurt, Wagscha] SDA Agr Res Serv, Western Reg Res Ctr, Albany, CA USA. [Charles, Lee] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S45 EP S45 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500100 ER PT J AU Moar, W Tom, C Gerrit, S Graham, H Bruce, H AF Moar, William Tom, Clark Gerrit, Segers Graham, Head Bruce, Hibbard TI dsRNA The Next Generation of Pyramided Insect-protection traits SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Moar, William; Tom, Clark; Gerrit, Segers; Graham, Head] Monsanto Co, Creve Coeur, MO 63167 USA. [Bruce, Hibbard] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S47 EP S48 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500106 ER PT J AU Niedz, R Evens, T AF Niedz, Randall Evens, Terence TI The Effect of Zeatin Riboside, BA, IAA, and NAA on Shoot Regeneration from Grapefruit Epicotyls SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Niedz, Randall; Evens, Terence] USDA, ARS, Ft Pierce, FL 34945 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S52 EP S52 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500115 ER PT J AU Niedz, R Evens, T AF Niedz, Randall Evens, Terence TI Determining Mineral Nutrient Effects using ARS-Media to Remove Ion Confounding SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Niedz, Randall; Evens, Terence] USDA, ARS, Ft Pierce, FL 34945 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S51 EP S51 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500114 ER PT J AU Ninkovic, S Nikolic, R Mitic, N Cingel, A Savic, J Djordjevic, T Smigocki, A AF Ninkovic, Slavica Nikolic, Radomirka Mitic, Nevana Cingel, Aleksandar Savic, Jelena Djordjevic, Tatjana Smigocki, Ann TI Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Ninkovic, Slavica; Nikolic, Radomirka; Mitic, Nevana; Cingel, Aleksandar; Savic, Jelena; Djordjevic, Tatjana] Inst Biol Res Dr Sinisa Stankovic, Belgrade 11060, Serbia. [Smigocki, Ann] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S191 EP S191 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500428 ER PT J AU Palla, K Pijut, P AF Palla, Kaitlin Pijut, Paula TI Regeneration of Plants from White Ash (Fraxinus americana L) Hypocotyls SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Palla, Kaitlin] Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Pijut, Paula] USDA Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S151 EP S151 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500337 ER PT J AU Park, W Bauer, P Scheffler, B Campbell, B AF Park, Wonkeun Bauer, Phil Scheffler, Brian Campbell, B. TI Drought-Related Gene Expression in Upland Cotton SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Park, Wonkeun; Bauer, Phil; Campbell, B.] USDA ARS, Florence, SC USA. [Scheffler, Brian] USDA ARS, Stoneville, MS 38776 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S152 EP S153 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500341 ER PT J AU Pijut, P AF Pijut, Paula TI Conservation and Genetic Improvement of Fine Hardwood Tree Species SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pijut, Paula] Purdue Univ, USDA, US Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S58 EP S59 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500130 ER PT J AU Preece, J West, T AF Preece, John West, Todd TI Microshoot Encapsulation for Cold Storage, Cleanup from Arthropod Infestations, and Acclimatization to the Ambient Environment SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Preece, John] Natl Clonal Germplasm Repository, Davis, CA USA. [Preece, John] Natl Arid Land Plant Genet Resource Unit, Parlier, CA USA. [Preece, John] Univ Calif Davis, USDA ARS, Davis, CA 95616 USA. [West, Todd] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S60 EP S60 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500134 ER PT J AU Pudota, B Wu, L Bethke, P Jiang, JM AF Pudota, Bhaskar Wu, Lei Bethke, Paul Jiang Jiming TI Light-Colored, Low Acrylamide Potato Chips SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pudota, Bhaskar; Wu, Lei; Jiang Jiming] Univ Wisconsin, Madison, WI 53706 USA. [Bethke, Paul] USDA ARS, Madison, WI 53706 USA. RI pudota, bhaskar/C-5896-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S155 EP S155 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500347 ER PT J AU Reed, B AF Reed, Barbara TI Techniques for Short- and Long-term Preservation of Plant Tissues and Organs SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Reed, Barbara] ARS, USDA, Corvallis, OR 97333 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S65 EP S65 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500143 ER PT J AU Reed, B Uchendu, E Muminova, M Traber, M AF Reed, Barbara Uchendu, Esther Muminova, Magfrat Traber, Maret TI Improving Shoot Tip Cryopreservation with Antioxidant and Antistress Compounds SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Reed, Barbara] ARS, USDA, Corvallis, OR 97333 USA. [Uchendu, Esther] Univ Guelph, Dept Plant Agr, Ontario Agr Coll, Guelph, ON N1G 2W1, Canada. [Muminova, Magfrat] Tashkent Chem Tech Inst, Dept Biotechnol, Tashkent, Uzbekistan. [Traber, Maret] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S64 EP S64 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500141 ER PT J AU Reed, B Wada, S DeNoma, J Evans, T Niedz, R AF Reed, Barbara Wada, Sugae DeNoma, Jeanine Evans, Terrence Niedz, Randall TI Improving In Vitro Mineral Nutrition for Diverse Pear Germplasm SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Reed, Barbara; DeNoma, Jeanine] ARS, USDA, Corvallis, OR 97333 USA. [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Evans, Terrence; Niedz, Randall] US Hort Res Lab, Hort & Breeding Unit, Ft Pierce, FL 34945 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S64 EP S64 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500142 ER PT J AU Rupp, J Cruz, L Fellers, J Trick, H AF Rupp, Jessica Cruz, Luisa Fellers, John Trick, Harold TI RNAi-Mediated Viral Resistance in Transgenic Wheat SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica; Cruz, Luisa; Trick, Harold] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Fellers, John] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S157 EP S158 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500353 ER PT J AU Scorza, R AF Scorza, Ralph TI Virus-resistant Plums through Genetic Engineering-from Lab to Market SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Scorza, Ralph] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S68 EP S68 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500151 ER PT J AU Singer Stacy Hily Jean-Michel Yang Yazhou Liu, ZR AF Singer Stacy Hily Jean-Michel Yang Yazhou Liu Zongrang TI Understanding and Harnessing Enhancer-promoter Interactions and Insulation Systems for Precisely Engineering Gene Function and Agronomic Traits in Plants SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Singer; Stacy; Hily; Jean-Michel; Yang; Yazhou; Liu Zongrang] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Yazhou] NW A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S44 EP S45 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500099 ER PT J AU Singh, A Liang, YC Reid, M Jiang, CZ AF Singh, Alka Liang Yin-Chih Reid, Michael Jiang Cai-Zhong TI Co-silencing Mirabilis Antiviral Protein Enables Virus-induced Gene Silencing Studies for Gene Functional Analysis in Mirabilis Jalapa SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Singh, Alka; Liang Yin-Chih; Reid, Michael] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Jiang Cai-Zhong] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S72 EP S73 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500160 ER PT J AU Stanley, D AF Stanley, David TI Eicosanoid Signaling in Insect Immunity SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Stanley, David] USDA ARS, BCIRL, Columbia, MO 65203 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S75 EP S75 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500165 ER PT J AU Thilmony, R Guttman, M Meridith, C AF Thilmony, Roger Guttman, Mara Meridith, Cook TI New Molecular Tools for Improved Crop Biotechnology SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Thilmony, Roger; Guttman, Mara; Meridith, Cook] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S78 EP S78 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500173 ER PT J AU Thomson, J Yau, YY Chan, R Thilmony, R AF Thomson, James Yau Yaun-Yeu Chan, Ronald Thilmony, Roger TI Development of Site-Specific Recombinase Technology for Precise Crop Plant Genome Modification SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Thomson, James; Chan, Ronald; Thilmony, Roger] USDA, WRRC, CIU, Albany, CA 94701 USA. [Yau Yaun-Yeu] Plant Gene Express Ctr, Albany, CA 94701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD SPR PY 2010 VL 46 SU S BP S79 EP S79 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 695JX UT WOS:000285367500175 ER PT J AU Klasson, KT Ledbetter, CA Wartelle, LH Lingle, SE AF Klasson, K. Thomas Ledbetter, Craig. A. Wartelle, Lynda H. Lingle, Sarah E. TI Feasibility of dibromochloropropane (DBCP) and trichloroethylene (TCE) adsorption onto activated carbons made from nut shells of different almond varieties SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Activated carbon; Almond shells; Steam activation; Trichloroethylene; Dibromochloropropane ID AGRICULTURAL BY-PRODUCTS; VOLATILE ORGANIC-COMPOUNDS; METALS REMEDIATION; ESTIMATED COST; AQUEOUS-PHASE; WATER-VAPOR; PYROLYSIS; REMOVAL; BIOMASS; GROUNDWATER AB Steam-activated carbons were made from shells from five different almond varieties ('Nonpareil,' Padre,' Tuono,"23-122,'and'Y120-74') and from a mix of almond types. The purpose of the work was to evaluate if the composition of shells had any effect on the performance of the final product. The shells contained the same level of cellulose, but differed in their lignin and hemicellulose composition. The yield of carbon from the shells ranged from 20% to 23.5%, indicating a loss of about 80% of initial mass. Regardless of the composition, the performance of the activated carbons made from the shells was very similar in a feasibility study. The carbons were found to have a capacity of 100-105 mg/g of carbon for the two contaminants tested (dibromochloropropane and trichloroethylene). The finding that the activated carbons performed equally well, regardless of source of almond shells, suggest that that carbons could be made from almonds shells with consistent quality. Published by Elsevier B.V. C1 [Klasson, K. Thomas; Wartelle, Lynda H.; Lingle, Sarah E.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Ledbetter, Craig. A.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Klasson, KT (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Thomas.Klasson@ars.usda.gov RI Abdul Karim, Siti Kartina/B-1998-2010; OI Klasson, K. Thomas/0000-0003-3358-3081 NR 61 TC 11 Z9 11 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2010 VL 31 IS 2 BP 261 EP 265 DI 10.1016/j.indcrop.2009.11.002 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA 574EY UT WOS:000275972700010 ER PT J AU Nakayama, FS Osbrink, WL AF Nakayama, F. S. Osbrink, W. L. TI Evaluation of kukui oil (Aleurites moluccana) for controlling termites SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Termite control; Kukui oil; Aleurites moluccana; Coptotermes formosanus ID FORMOSAN SUBTERRANEAN TERMITE; ISOPTERA; RHINOTERMITIDAE; FLORIDA AB The application of many chemical-based pesticides to protect wood has been greatly restricted in the United States and elsewhere. A possible natural product that can be used for wood preservation is the oil from the nut of the kukui plant, Aleurites moluccana (L.) Willd., which has been reported, based on native folklore, to protect canoes against marine borer damage. The objective of this study was to determine whether the kukui oil would have termite control properties. Oil obtained by mechanical pressing of the nut from the kukui plant was used to treat southern yellow pine (Pines spp.) wood. Wood blocks were impregnated with various mixtures of the oil and acetone using a vacuum-pressure-infiltration chamber to attain a range of oil contents in the wood. Laboratory studies with the Formosan termite (Coptotermes formosanus) showed that the oil-treated wood was resistant to termite damage when the wood contained >27% kukui oil by weight. Results also indicated that the oil acted primarily as a feeding deterrent and not a toxic agent. Published by Elsevier B.V. C1 [Nakayama, F. S.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Osbrink, W. L.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Nakayama, FS (reprint author), USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM francis.nakayama@ars.usda.gov NR 19 TC 10 Z9 11 U1 4 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2010 VL 31 IS 2 BP 312 EP 315 DI 10.1016/j.indcrop.2009.11.009 PG 4 WC Agricultural Engineering; Agronomy SC Agriculture GA 574EY UT WOS:000275972700017 ER PT J AU Finkenstadt, VL Tisserat, B AF Finkenstadt, Victoria L. Tisserat, Brent TI Poly(lactic acid) and Osage Orange wood fiber composites for agricultural mulch films SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Polymer; Composite; Wood; Poly(lactic acid); Mulch film ID GREEN COMPOSITES; MACLURA-POMIFERA; MECHANICAL-PROPERTIES; LACTIC-ACID; ROOT BARK; POLYMERS; BIODEGRADATION; IMPACT; PLANT AB Osage Orange wood (OO) was combined with poly(lactic acid) (PTA) to form a polymer composite intended for use as an agricultural mulch film. The PLA-OO mechanical properties were comparable to existing mulch film products and had the advantage of being completely biodegradable through a single growing season. PLA-OO composites were evaluated for mechanical properties in both dry and wet conditions. The availability of its organic components useful for release into the environment was determined to be significantly higher than native OO before thermomechanical treatment. PLA-OO mulch films will provide a weed barrier and control soil erosion, as well as allowing the controlled release of the OO phytochemical components for additional protection. Published by Elsevier B.V. C1 [Finkenstadt, Victoria L.] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Tisserat, Brent] ARS, New Crops & Proc Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Finkenstadt, VL (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM victoria.finkenstadt@ars.usda.gov NR 28 TC 22 Z9 24 U1 1 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2010 VL 31 IS 2 BP 316 EP 320 DI 10.1016/j.indcrop.2009.11.012 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA 574EY UT WOS:000275972700018 ER PT J AU Webb, KM Rosenthal, BM AF Webb, Kristen M. Rosenthal, Benjamin M. TI Deep resequencing of Trichinella spiralis reveals previously un-described single nucleotide polymorphisms and intra-isolate variation within the mitochondrial genome SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Trichinella spiralis; Mitochondrial genome; Next generation sequencing; Intra-isolate variability ID DNA; EVOLUTION; SYSTEMATICS; GENETICS AB The phylogeny and historical dispersal of Trichinella spp. have been studied, in part, by sequencing portions of the mitochondrial genome. Such studies rely on two untested beliefs: that variation in a portion is representative of the entire mitochondrial genome, and that each isolate is characterized by only one mitochondrial haplotype. We have used next generation DNA sequencing technology to obtain the complete mitochondrial genome sequence from a second isolate of T. spiralis. By aligning it to the only previously sequenced genome, we sought to establish whether the exceptionally deep sequencing coverage provided by such an approach could detect regions of the genome which had been misassembled, or nucleotide positions which may vary within an isolate. The new data broadly confirm the gene order and sequence assembly for protein-coding regions. However, in the repetitive non-coding region, alignment to the previously published genome sequence proved difficult. Such discrepancies may represent true biological variation, but may rather result from methodological or algorithmic sources. Within the 13,902 bp protein-coding region, 7 polymorphisms were identified. Six of these polymorphisms occurred within protein-coding genes and three alter an amino acid sequence, one occurred in a tRNA-Ile sequence, and four were found to vary within our isolate. Thus, comparing only two isolates of T. spiral's has enabled the discovery of previously unrecognized variation within the species. Characterizing diversity within and among the mitochondrial genomes of additional species of Trichinella would undoubtedly yield further insights into the diversification history of the genus. Our study affirms that next generation DNA sequencing technology can reliably characterize a complete mitochondrial genome. (C) 2010 Elsevier B.V. All rights reserved. C1 [Webb, Kristen M.; Rosenthal, Benjamin M.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Rosenthal, BM (reprint author), USDA ARS, Anim Parasit Dis Lab, Bldg 1180,BARC E, Beltsville, MD 20705 USA. EM Benjamin.Rosenthal@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 FU ARS Research Associate Program [1265-42000-011] FX KM Webb is supported by the ARS Research Associate Program.; This work was funded by ARS project 1265-42000-011, "Molecular Systematics and Comparative Population Genetics of Parasitic Organisms That Threaten Food Safety and Security." We thank Alicia Beavers, Tony Capuco, Chris Clover, Detiger Dunams, Monica Santin-Duran, Steve Schroeder and Tad Sonstegard for support towards the completion of the project. We also thank Dante Zarlenga for providing isolates ISS #31 and ISS #4 as well as for thoughtful technical guidance. NR 18 TC 13 Z9 13 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD MAR PY 2010 VL 10 IS 2 BP 304 EP 310 DI 10.1016/j.meegid.2010.01.003 PG 7 WC Infectious Diseases SC Infectious Diseases GA 573PJ UT WOS:000275926400020 PM 20083232 ER PT J AU Vincent, AL Lager, KM Faaberg, KS Harland, M Zanella, EL Ciacci-Zanella, JR Kehrli, ME Janke, BH Klimov, A AF Vincent, Amy L. Lager, Kelly M. Faaberg, Kay S. Harland, Michelle Zanella, Eraldo L. Ciacci-Zanella, Janice R. Kehrli, Marcus E., Jr. Janke, Bruce H. Klimov, Alexander TI Experimental inoculation of pigs with pandemic H1N1 2009 virus and HI cross-reactivity with contemporary swine influenza virus antisera SO INFLUENZA AND OTHER RESPIRATORY VIRUSES LA English DT Article DE H1N1 2009; hemagglutination inhibition assay; influenza A virus; pathogenesis; swine ID A VIRUSES; UNITED-STATES; REASSORTANT H1N2; ORIGIN H1N1; HUMANS; CANADA; IDENTIFICATION; RECEPTORS; INFECTION; PCR AB Background A novel A/H1N1 was identified in the human population in North America in April 2009. The gene constellation of the virus was a combination from swine influenza A viruses (SIV) of North American and Eurasian lineages that had never before been identified in swine or other species. Objectives The objectives were to (i) evaluate the clinical response of swine following experimental inoculation with pandemic H1N1 2009; (ii) assess serologic cross-reactivity between H1N1 2009 and contemporary SIV antisera; and (iii) develop a molecular assay to differentiate North American-lineage SIV from H1N1 2009. Methods Experiment 1: Weaned pigs were experimentally infected with A/California/04/2009 (H1N1). Experiment 2: The cross-reactivity of a panel of US SIV H1N1 or H1N2 antisera with three isolates of pandemic A/H1N1 was evaluated. Experiment 3: A polymerase chain reaction (PCR)-based diagnostic test was developed and validated on samples from experimentally infected pigs. Results and Conclusions In experiment 1, all inoculated pigs demonstrated clinical signs and lesions similar to those induced by endemic SIV. Viable virus and antigen were only detected in the respiratory tract. In experiment 2, serologic cross-reactivity was limited against H1N1 2009 isolates, notably among virus antisera from the same HA phylogenetic cluster. The limited cross-reactivity suggests North American pigs may not be fully protected against H1N1 2009 from previous exposure or vaccination and novel tests are needed to rapidly diagnose the introduction of H1N1 2009. In experiment 3, an RT-PCR test that discriminates between H1N1 2009 and endemic North American SIV was developed and validated on clinical samples. C1 [Vincent, Amy L.; Lager, Kelly M.; Faaberg, Kay S.; Harland, Michelle; Zanella, Eraldo L.; Ciacci-Zanella, Janice R.; Kehrli, Marcus E., Jr.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Zanella, Eraldo L.] Univ Passo Fundo, Fac Agron & Med Vet, BR-1920 Passo Fundo, RS, Brazil. [Ciacci-Zanella, Janice R.] EMBRAPA Brazilian Agr Res Corp, Labex USA, Brasilia, DF, Brazil. [Janke, Bruce H.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Klimov, Alexander] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA USA. RP Vincent, AL (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov RI Zanella, Janice/C-3632-2014 FU USDA-ARS; DHHS-CDC FX The authors thank David Michael and Hillary Horst for technical assistance and Dr Becky Jepsen, Brian Pottebaum, and Jason Huegel for assistance with animal studies. We thank Dr Crystal Loving for critical review of the manuscript and Dr Marie Gramer (University of Minnesota) for providing SIV isolates. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. Funding was provided by USDA-ARS and DHHS-CDC. NR 26 TC 52 Z9 53 U1 1 U2 5 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1750-2640 J9 INFLUENZA OTHER RESP JI Influenza Other Respir. Viruses PD MAR PY 2010 VL 4 IS 2 BP 53 EP 60 DI 10.1111/j.1750-2659.2009.00121.x PG 8 WC Infectious Diseases; Virology SC Infectious Diseases; Virology GA 554BR UT WOS:000274411000002 PM 20167045 ER PT J AU Jasrapuria, S Arakane, Y Osman, G Kramer, KJ Beeman, RW Muthukrishnan, S AF Jasrapuria, Sinu Arakane, Yasuyuki Osman, Gamal Kramer, Karl J. Beeman, Richard W. Muthukrishnan, Subbaratnam TI Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny, expression and function SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Chitin; Chitin-binding domain; Cuticle; Epidermis; Gasp; Midgut; Peritrophic matrix; Peritrophin A domain; Obstructors; Phylogenetic analysis; Red flour beetle; Tribolium castaneum ID MANDUCA-SEXTA; DROSOPHILA-MELANOGASTER; SPODOPTERA-FRUGIPERDA; CUTICULAR PROTEINS; STRUCTURAL MODEL; INTESTINAL MUCIN; INSECT CHITINASE; MEMBRANE; CUTICLE; MATRIX AB This study is focused on the characterization and expression of genes in the red flour beetle, Tribolium castaneum, encoding proteins that possess one or more six-cysteine-containing chitin-binding domains related to the peritrophin A domain (ChtBD2). An exhaustive bioinformatics search of the genome of T castaneum queried with ChtBD2 sequences yielded 13 previously characterized chitin metabolic enzymes and 29 additional proteins with signal peptides as well as one to 14 ChtBD2s. Using phylogenetic analyses, these additional 29 proteins were classified into three large families. The first family includes 11 proteins closely related to the peritrophins, each containing one to 14 ChtBD2s. These are midgut-specific and are expressed only during feeding stages. We propose the name "Peritrophic Matrix Proteins" (PMP) for this family. The second family contains eight proteins encoded by seven genes (one gene codes for 2 splice variants), which are closely related to gasp/obstructor-like proteins that contain 3 ChtBD2s each. The third family has ten proteins that are of diverse sizes and sequences with only one ChtBD2 each. The genes of the second and third families are expressed in non-midgut tissues throughout all stages of development. We propose the names "Cuticular Proteins Analogous to Peritophins 3" (CPAP3) for the second family that has three ChtBD2s and "Cuticular Proteins Analogous to Peritophins 1 (CPAP1) for the third family that has 1 ChtBD2. Even though proteins of both CPAP1 and CPAP3 families have the "peritrophin A" domain, they are expressed only in cuticle-forming tissues. We determined the exon intron organization of the genes, encoding these 29 proteins as well as the domain organization of the encoded proteins with ChtBD2s. All 29 proteins have predicted cleavable signal peptides and ChtBD2s, suggesting that they interact with chitin in extracellular locations. Comparison of ChtBD2s-containing proteins in different insect species belonging to different orders suggests that ChtBD2s are ancient protein domains whose affinity for chitin in extracellular matrices has been exploited many times for a range of biological functions. The differences in the expression profiles of PMPs and CPAPs indicate that even though they share the peritrophin A motif for chitin binding, these three families of proteins have quite distinct biological functions. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Jasrapuria, Sinu; Arakane, Yasuyuki; Kramer, Karl J.; Muthukrishnan, Subbaratnam] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Osman, Gamal] Adv Genet Engn Res Inst, Giza, Egypt. [Kramer, Karl J.; Beeman, Richard W.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Osman, Gamal] Umm Al Qura Univ, Dept Biol, Mecca, Saudi Arabia. RP Muthukrishnan, S (reprint author), Kansas State Univ, Dept Biochem, 141 Chalmers Hall, Manhattan, KS 66506 USA. EM smk@ksu.edu FU NSF [IBN-0316963, IOS-615818] FX We thank Kathy Leonard for beetle husbandry and Dr. Qingsong Zhu for some of the preliminary work on CPAP3. We thank Neal Dittmer for his critical review of this paper and for valuable suggestions. This project was supported by NSF grants IBN-0316963 and IOS-615818. This is contribution no. 10-115-J from the Kansas Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 47 TC 63 Z9 67 U1 1 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAR PY 2010 VL 40 IS 3 SI SI BP 214 EP 227 DI 10.1016/j.ibmb.2010.01.011 PG 14 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 597KB UT WOS:000277754700006 PM 20144715 ER PT J AU Suderman, RJ Dittmer, NT Kramer, KJ Kanost, MR AF Suderman, Richard J. Dittmer, Neal T. Kramer, Karl J. Kanost, Michael R. TI Model reactions for insect cuticle sclerotization: Participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36 SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Alanine; Catecholamine; Cross-link; Cuticle; Dityrosine; Histidine; Insect; Laccase; Lysine; Protein multimers; Sclerotization; Site-directed mutagenesis; Succinylation; Tyrosine ID PHARATE PUPAL CUTICLE; CUTICULAR SCLEROTIZATION; OXIDATIVE CONJUGATION; N-ACETYLHISTIDINE; TOBACCO HORNWORM; CATECHOLAMINE; DITYROSINE; MECHANISM; PRODUCTS; QUINONES AB Current theories of sclerotization center on protein cross-linking and dehydration as major factors in the hardening and stability of the insect cuticle. Several studies have reported the identification of catechol-amino acid adducts from sclerotizing cuticle involving histidine, lysine, and tyrosine, though there have been no reports of a catechol linked between two amino acid residues. Previously, we reported an in vitro model system for sclerotization and observed that stable protein oligomers were formed, presumably through cross-links with oxidized catecholamines [Insect Biochem. Mol. Biol. (2006) 36, 353-365]. Using site-directed mutagenesis we created a mutant lacking histidine, rMsCP36(H65A/H178A), to investigate the possible involvement of the two histidine residues of MsCP36 in cross-linking. Surprisingly, this alteration had little or no effect on the formation of protein oligomers as determined by SDS-PAGE analysis. Blocking of the free amino groups in lysyl side chains and the amino-terminus by succinylation diminished, but did not eliminate, cross-linking of either rM5CP36 or rMsCP36(H65A/H178A). We also examined the possibility that cross-linking was due to intermolecular dityrosine linkages. Immunoblot analysis utilizing a monoclonal antibody known to recognize peptidyl dityrosine indicated that dityrosyl cross-links were present. Taken together, these results indicate that lysyl residues are important for the cross-linking of the cuticle protein rMsCP36, but that additional residues other than histidine can also contribute. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Suderman, Richard J.; Dittmer, Neal T.; Kramer, Karl J.; Kanost, Michael R.] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Kramer, Karl J.] ARS, Grain Mkt & Prod Res Ctr, USDA, Manhattan, KS 66502 USA. RP Dittmer, NT (reprint author), Kansas State Univ, Dept Biochem, 141 Chalmers Hall, Manhattan, KS 66506 USA. EM ndittmer@ksu.edu FU National Science Foundation [IOS 0726425] FX This research was supported by a grant from the National Science Foundation (IOS 0726425), and is contribution number 10-099-J from the Kansas Agricultural Experiment Station. We are grateful to Dr. Yoji Kato, University of Hyogo, Japan for supplying us with the dityrosine antibody. Mention of a proprietary product does not constitute a recommendation or endorsement by the USDA. The USDA is an equal opportunity/affirmative action employer and all agency services are available without discrimination. NR 29 TC 9 Z9 10 U1 1 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAR PY 2010 VL 40 IS 3 SI SI BP 252 EP 258 DI 10.1016/j.ibmb.2010.02.008 PG 7 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 597KB UT WOS:000277754700010 PM 20219676 ER PT J AU Arakane, Y Dittmer, NT Tomoyasu, Y Kramer, KJ Muthukrishnan, S Beeman, RW Kanost, MR AF Arakane, Yasuyuki Dittmer, Neal T. Tomoyasu, Yoshinori Kramer, Karl J. Muthukrishnan, Subbaratnam Beeman, Richard W. Kanost, Michael R. TI Identification, mRNA expression and functional analysis of several yellow family genes in Tribolium castaneum SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Tribolium castaneum; RNA interference; yellow; Pigmentation; Sclerotization; Drosophila melanogaster; Pterostigma; Dopachrome conversion enzyme; Tanning; Cuticle; Melanin ID BACTERIUM DEINOCOCCUS-RADIODURANS; MELANIN-SYNTHESIS ENZYMES; ROYAL JELLY PROTEINS; DROSOPHILA-MELANOGASTER; PIGMENT PATTERNS; MANDUCA-SEXTA; BOMBYX-MORI; REGULATORY EVOLUTION; ADULT DEVELOPMENT; PAPILIO-XUTHUS AB Querying the genome of the red flour beetle. Tribolium castaneum, with the Drosophila melanogaster Yellow-y (DmY-y) protein sequence identified 14 Yellow homologs. One of these is an ortholog of DmY-y, which is required for cuticle pigmentation (melanization), and another is an ortholog of DmY-f/f2, which functions as a dopachrome conversion enzyme (DCE). Phylogenetic analysis identified putative T. castaneum orthologs for eight of the D. melanogaster yellow genes, including DmY-b, -c, -e, -f, -g, -g2, -h and -y. However, one clade of five beetle genes, TcY-1-5, has no orthologs in D. melanogaster. Expression profiles of all T. castaneum yellow genes were determined by RT-PCR of pharate pupal to young adult stages. TcY-b and TcY-c were expressed throughout all developmental stages analyzed, whereas each of the remaining yellow genes had a unique expression pattern, suggestive of distinct physiological functions. TcY-b, -c and - e were all identified by mass spectrometry of elytral proteins from young adults. Eight of the 14 genes showed differential expression between elytra and hindwings during the last three days of the pupal stage when the adult cuticle is synthesized. Double-stranded RNA (dsRNA)-mediated transcript knockdown revealed that TcY-y is required for melanin production in the hindwings, particularly in the region of the pterostigma, while TcY-f appears to be required for adult cuticle sclerotization but not pigmentation. (C) 2010 Elsevier Ltd. All rights reserved. C1 [Arakane, Yasuyuki; Dittmer, Neal T.; Kramer, Karl J.; Muthukrishnan, Subbaratnam; Kanost, Michael R.] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Tomoyasu, Yoshinori] Miami Univ, Dept Zool, Oxford, OH USA. [Kramer, Karl J.; Beeman, Richard W.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66506 USA. RP Arakane, Y (reprint author), Kansas State Univ, Dept Biochem, 141 Chalmers Hall, Manhattan, KS 66506 USA. EM subaru@ksu.edu RI Tomoyasu, Yoshinori/D-3061-2017 OI Tomoyasu, Yoshinori/0000-0001-9824-3454 FU Arthropod Genomics Seed [NOBO2-83015]; National Science Foundation [IOS 0726425] FX We thank the Kendrick Labs for performing the 2D gel electrophoresis, Dr. Yasuaki Hiromasa for the peptide mass fingerprinting analysis, Dr. Yoonseong Park for design of the microarray, Dr. Nigel Cooper and Ms Xiaohong Li for the microarray hybridization, and Ms. Nanyan Lu for assisting with the microarray statistical analysis. This work was supported by Arthropod Genomics Seed Grant NOBO2-83015 and National Science Foundation Grant IOS 0726425. This is contribution 10-104-J from the Kansas Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply a recommendation or endorsement by the U.S. Department of Agriculture. NR 46 TC 18 Z9 19 U1 1 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAR PY 2010 VL 40 IS 3 SI SI BP 259 EP 266 DI 10.1016/j.ibmb.2010.01.012 PG 8 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 597KB UT WOS:000277754700011 PM 20149870 ER PT J AU Broehan, G Arakane, Y Beeman, RW Kramer, KJ Muthukrishnan, S Merzendorfer, H AF Broehan, Gunnar Arakane, Yasuyuki Beeman, Richard W. Kramer, Karl J. Muthukrishnan, Subbaratnam Merzendorfer, Hans TI Chymotrypsin-like peptidases from Tribolium castaneum: A role in molting revealed by RNA interference SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Chymotrypsin; Serine peptidases; Midgut; Cuticle; Molting fluid; RNA interference; Tribolium castaneum; Coleoptera; Red flour beetle ID RED FLOUR BEETLE; TOBACCO HORNWORM; MANDUCA-SEXTA; SERINE PROTEASES; HELICOVERPA-ARMIGERA; COTTON BOLLWORM; SUBSTRATE-SPECIFICITY; PROTEINASE-INHIBITORS; MOLECULAR-CLONING; CHITIN SYNTHASE AB Chymotrypsin-like peptidases (CTLPs) of insects are primarily secreted into the gut lumen where they act as digestive enzymes. We studied the gene family encoding CTLPs in the genome of the red flour beetle, Tribolium castaneum. Using an extended search pattern, we identified 14 TcCTLP genes that encode peptidases with Si specificity pocket residues typically found in chymotrypsin-like enzymes. We further analyzed the expression patterns of seven TcCTLP genes at various developmental stages. While some TcCTLP genes were exclusively expressed in feeding larval and adult stages (TcCTLP-5A/B, TcCTLP-6A), others were also detected in non-feeding embryonic (TcCTLP-5C, TcCTLP-6D) and pupal stages (TcCTLP-5C, TcCTLP-6C/D/E). TcCTLP genes were expressed predominantly in the midgut, where they presumably function in digestion. However, TcCTLP-6C and TcCTLP-5C also showed considerable expression in the carcass. The latter two genes might therefore encode peptidases that act as molting fluid enzymes. To test this hypothesis, we performed western blots using protein extracts from larval exuviae. The extracts reacted with antibodies to TcCTLP-5C and TcCTLP-6E suggesting that the corresponding peptidases are secreted into the molting fluid. Finally, we performed systemic RNAi experiments. While injections of five TcCTLP-dsRNAs into penultimate larvae did not affect growth or development, injection of dsRNA for TcCTLP-5C and TcCTLP-6C resulted in severe molting defects. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Broehan, Gunnar; Merzendorfer, Hans] Univ Osnabruck, Dept Biol Chem, Div Anim Physiol, D-49069 Osnabruck, Germany. [Arakane, Yasuyuki; Kramer, Karl J.; Muthukrishnan, Subbaratnam] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Beeman, Richard W.; Kramer, Karl J.] ARS, USDA, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. RP Merzendorfer, H (reprint author), Univ Osnabruck, Dept Biol Chem, Div Anim Physiol, Barbarastr 11, D-49069 Osnabruck, Germany. EM merzendorfer@biologie.uni-osnabrueck.de FU Deutsche Forschungsgemeinschaft [SFB 431, GRK 612]; National Science Foundation [IOS-0615818] FX This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB 431 and GRK 612) and National Science Foundation (IOS-0615818). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 50 TC 16 Z9 19 U1 2 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAR PY 2010 VL 40 IS 3 SI SI BP 274 EP 283 DI 10.1016/j.ibmb.2009.10.009 PG 10 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 597KB UT WOS:000277754700013 PM 19897036 ER PT J AU Fagerstone, KA Miller, LA Killian, G Yoder, CA AF Fagerstone, Kathleen A. Miller, Lowell A. Killian, Gary Yoder, Christi A. TI Review of issues concerning the use of reproductive inhibitors, with particular emphasis on resolving human-wildlife conflicts in North America SO INTEGRATIVE ZOOLOGY LA English DT Review DE contraceptives; fertility control; registration; reproductive inhibitors; wildlife ID WHITE-TAILED DEER; GONADOTROPIN-RELEASING-HORMONE; REMOTELY-DELIVERED IMMUNOCONTRACEPTION; PORCINE ZONAE PELLUCIDAE; CONJUGATED LINOLEIC-ACID; FERTILITY-CONTROL; MELENGESTROL ACETATE; GNRH VACCINE; PLASMA-CONCENTRATIONS; ESTROUS CYCLES AB This manuscript provides an overview of past wildlife contraception efforts and discusses the current state of research. Two fertility control agents, an avian reproductive inhibitor containing the active ingredient nicarbazin and an immunocontraceptive vaccine, have received regulatory approval with the Environmental Protection Agency and are commercially available in the USA. OvoControl G Contraceptive Bait for Canada Geese and Ovo Control for pigeons are delivered as oral baits. An injectable immunocontraceptive vaccine (GonaCon Immunocontraceptive Vaccine) was registered with the Environmental Protection Agency for use in female white-tailed deer in September 2009. An injectable product (GonaCon Immunocontraceptive Vaccine) is registered for use in female white-tailed deer. Both products are labeled for use in urban/suburban areas where these species are overabundant. Several other compounds are currently being tested for use in wildlife in the USA, Europe, Australia and New Zealand that could have promise in the future. The development and use of reproductive inhibitors for resolving human-wildlife conflicts will depend on a number of factors, including meeting the requirements of regulatory agencies for use in the environment and on the biological and economical feasibility of their use. Use will also be dependent on health and safety issues and on public acceptance of the techniques. C1 [Fagerstone, Kathleen A.; Miller, Lowell A.; Yoder, Christi A.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Killian, Gary] Penn State Univ, Almquist Res Ctr, University Pk, PA 16802 USA. RP Fagerstone, KA (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM kathleen.a.fagerstone@aphis.usda.gov NR 118 TC 30 Z9 32 U1 4 U2 52 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1749-4877 J9 INTEGR ZOOL JI Integr. Zool. PD MAR PY 2010 VL 5 IS 1 BP 15 EP 30 DI 10.1111/j.1749-4877.2010.00185.x PG 16 WC Zoology SC Zoology GA 582QA UT WOS:000276611500003 PM 21392318 ER PT J AU Archibong, AE Ramesh, A Niaz, MS Brooks, CM Roberson, SI Lunstra, DD AF Archibong, Anthony E. Ramesh, Aramandla Niaz, Mohammad S. Brooks, Cynthia M. Roberson, Shannon I. Lunstra, Donald D. TI Effects of Benzo(a) pyrene on Intra-testicular Function in F-344 Rats (vol 5, pg 32, 2008) SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Correction C1 [Archibong, Anthony E.; Niaz, Mohammad S.; Brooks, Cynthia M.; Roberson, Shannon I.] Meharry Med Coll, Dept Obstet & Gynecol, Nashville, TN 37208 USA. [Ramesh, Aramandla] Meharry Med Coll, Dept Biochem & Canc Biol, Nashville, TN 37208 USA. [Lunstra, Donald D.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Archibong, AE (reprint author), Meharry Med Coll, Dept Obstet & Gynecol, Nashville, TN 37208 USA. EM aarchibong@mmc.edu NR 1 TC 0 Z9 0 U1 0 U2 1 PU MOLECULAR DIVERSITY PRESERVATION INTERNATIONAL-MDPI PI BASEL PA KANDERERSTRASSE 25, CH-4057 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD MAR PY 2010 VL 7 IS 3 BP 1224 EP 1225 PG 2 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA 573XZ UT WOS:000275951600031 ER PT J AU Liu, CH Zheng, XZ Shi, J Xue, J Lan, YB Jia, SH AF Liu, Chenghai Zheng, Xianzhe Shi, John Xue, Jun Lan, Yubin Jia, Shuhua TI Optimising microwave vacuum puffing for blue honeysuckle snacks SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Blue honeysuckle; microwave vacuum; puffing; snack ID RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION; PARAMETERS AB Fresh blue honeysuckle fruit slices were puffed in a microwave vacuum dryer up to a final moisture content about 5% (w.b.). The effect of initial moisture content (IMC) (25-45%), vacuum pressure (VP) (70-90 kPa) and microwave intensity (MI) (10-30 W g-1) on quality attributes, in terms of expansion ratio (ER), hardness (HD), crispness (CR) and colour of the products, were analysed by response surface methodology. Besides the effect of MI on chroma (CH), the high IMC and low VP had a significantly positive impact on the quality attributes of blue honeysuckle snacks. The optimum product qualities, which were ER (1.62 times), HD (5836.31 g), CR (4.48), and CH (28.7) were obtained at an IMC of 38.42%, VP of 82.02 kPa, and MI of 22.42 W g-1. The microwave vacuum method has obvious advantages when puffing the blue honeysuckle snacks. C1 [Liu, Chenghai; Zheng, Xianzhe; Jia, Shuhua] NE Agr Univ, Coll Engn, Harbin 150030, Peoples R China. [Shi, John; Xue, Jun] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada. [Lan, Yubin] ARS, USDA, College Stn, TX 77845 USA. RP Zheng, XZ (reprint author), NE Agr Univ, Coll Engn, Harbin 150030, Peoples R China. EM zhengxz@neau.edu.cn NR 23 TC 13 Z9 16 U1 1 U2 10 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0950-5423 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD MAR PY 2010 VL 45 IS 3 BP 506 EP 511 DI 10.1111/j.1365-2621.2009.02156.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 554MK UT WOS:000274438900011 ER PT J AU Carrillo, AC Thissen, J Olivas, J Pitz, K El Sheikh, M Harrel, B Hall, S Rasmussen, M Tammero, LB Lenhoff, R Arani, PN AF Carrillo, A. C. Thissen, J. Olivas, J. Pitz, K. El Sheikh, M. Harrel, B. Hall, S. Rasmussen, M. Tammero, L. Bentley Lenhoff, R. Arani, P. Naraghi TI Multiplexed diagnostic assays for detection of high consequence foreign and emerging animal disease SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Meeting Abstract C1 [Carrillo, A. C.; Thissen, J.; Olivas, J.; Pitz, K.; El Sheikh, M.; Harrel, B.; Hall, S.; Tammero, L. Bentley; Lenhoff, R.; Arani, P. Naraghi] Lawrence Livermore Natl Lab, Livermore, CA USA. [Rasmussen, M.] USDA ARS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD MAR PY 2010 VL 14 SU 1 BP E373 EP E374 DI 10.1016/j.ijid.2010.02.451 PG 2 WC Infectious Diseases SC Infectious Diseases GA 578MQ UT WOS:000276298201411 ER PT J AU Cheema, A Farrell, J Kurtzman, C AF Cheema, A. Farrell, J. Kurtzman, C. TI Saccharomyces fungemia associated with esophageal disease identified by D1/D2 Ribosomal RNA gene sequence SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Meeting Abstract C1 [Cheema, A.] Univ Illinois, Coll Med, Peoria, IL 61656 USA. [Farrell, J.] OSF St Francis, Peoria, IL USA. [Kurtzman, C.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD MAR PY 2010 VL 14 SU 1 BP E126 EP E127 DI 10.1016/j.ijid.2010.02.1765 PG 2 WC Infectious Diseases SC Infectious Diseases GA 578MQ UT WOS:000276298200286 ER PT J AU Stanek, D Nicoletti, P Harrell, D Churchwell, G Lee, P Blackmore, C AF Stanek, D. Nicoletti, P. Harrell, D. Churchwell, G. Lee, P. Blackmore, C. TI Epidemiologic comparison of Human Brucella suis and Brucella melitensis infections SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Meeting Abstract C1 [Stanek, D.; Blackmore, C.] Florida Dept Hlth, Tallahassee, FL USA. [Nicoletti, P.] Univ Florida, Coll Vet Med, Gainesville, FL USA. [Harrell, D.] USDA VS, Gainesville, FL USA. [Churchwell, G.; Lee, P.] FL DOH, Jacksonville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD MAR PY 2010 VL 14 SU 1 BP E212 EP E212 DI 10.1016/j.ijid.2010.02.1958 PG 1 WC Infectious Diseases SC Infectious Diseases GA 578MQ UT WOS:000276298201047 ER PT J AU Jiang, Y Jeon, HY Tian, L Bode, LE AF Jiang, Yan Jeon, Hong Young Tian, Lei Bode, Loren E. TI Measuring particle size distribution using LED-illumination SO INTERNATIONAL JOURNAL OF MULTIPHASE FLOW LA English DT Article DE LED-illumination; Droplet size distribution; Particle/droplet image analysis; Upper-limit lognormal distribution ID APPLICATION UNIFORMITY AB A particle/droplet image analysis (PDIA) system employing LED-illumination was designed Freezing the moving droplets using high speed camera instead of stroboscopic illumination, the system had no requirement to synchronize the backlight and the camera It featured low cost, low power consumption and simple optical configuration in comparison to laser-based systems Only focused droplets in images were Counted Given the sample size of the system was relatively small, an upper-limit lognormal distribution was used to fit the actual data to represent the spray patterns The accuracy of the diameter measurement was verified using precisely manufactured balls Comparisons with two laser-based systems were given and the system's capacity to distinct spray patterns were demonstrated in nozzle classification experiments (C) 2009 Elsevier Ltd All rights reserved. C1 [Jiang, Yan; Tian, Lei; Bode, Loren E.] Univ Illinois, Dept Agr Biol Engn, Urbana, IL 61801 USA. [Jiang, Yan] BeiHang Univ, Sch Transportat Sci & Engn, Beijing 100191, Peoples R China. [Jeon, Hong Young] USDA ARS, Wooster, OH 44691 USA. RP Jiang, Y (reprint author), Univ Illinois, Dept Agr Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. NR 15 TC 4 Z9 4 U1 0 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0301-9322 J9 INT J MULTIPHAS FLOW JI Int. J. Multiph. Flow PD MAR PY 2010 VL 36 IS 3 BP 193 EP 201 DI 10.1016/j.ijmultiphaseflow.2009.11.004 PG 9 WC Mechanics SC Mechanics GA 569QE UT WOS:000275612200004 ER PT J AU Chavez, JL Pierce, FJ Evans, RG AF Chavez, Jose L. Pierce, Francis J. Evans, Robert G. TI Compensating inherent linear move water application errors using a variable rate irrigation system SO IRRIGATION SCIENCE LA English DT Review DE Site specific irrigation; Linear move irrigation advance pattern; Water application coefficient of uniformity; Water application distribution uniformity AB Continuous move irrigation systems such as linear move and center pivot irrigate unevenly when applying conventional uniform water rates due to the towers/motors stop/advance pattern. The effect of the gear drive/cart movement pattern on linear move water application is larger on the first two spans, which introduces errors on site-specific irrigation. Therefore, the objectives of this study were to model the linear move irrigation system cart movement and to develop an algorithm to compensate for unintended variable irrigation (application errors). The cart advance/movement modeling considered terrain attributes, average nozzle travel speed, and high frequency DGPS (differential global positioning system) cart positioning readings. This paper describes the use of an irrigation monitoring and control system, DGPS, GIS, and statistical analysis utilized in the modeling and compensation processes. The irrigation monitoring and control system was composed of a single board computer, a relay board controller, DGPS, electric solenoid valves, wireless ethernet bridge units, high frequency spread spectrum radios, as well as in-line and in-field sensor networks. This technology allowed for continuous, real-time data acquisition and irrigation system management through the internet. This study has shown that irrigation application errors were reduced from over 20% to around 5%, in the subsequent irrigation event. C1 [Chavez, Jose L.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Pierce, Francis J.] Washington State Univ, Ctr Precis Agr Syst, Prosser, WA USA. [Evans, Robert G.] USDA ARS, No Plains Agr Res Lab, Sidney, MT USA. RP Chavez, JL (reprint author), Colorado State Univ, Dept Civil & Environm Engn, 1372 Campus Delivery, Ft Collins, CO 80523 USA. EM jose.chavez@colostate.edu; fjpierce@wsu.edu; robert.evans@ars.usda.gov NR 13 TC 6 Z9 6 U1 1 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 J9 IRRIGATION SCI JI Irrig. Sci. PD MAR PY 2010 VL 28 IS 3 BP 203 EP 210 DI 10.1007/s00271-009-0188-6 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 551JX UT WOS:000274205300001 ER PT J AU Williams, LE Grimes, DW Phene, CJ AF Williams, L. E. Grimes, D. W. Phene, C. J. TI The effects of applied water at various fractions of measured evapotranspiration on water relations and vegetative growth of Thompson Seedless grapevines SO IRRIGATION SCIENCE LA English DT Article ID VITIS-VINIFERA L; DEFICIT IRRIGATION; SHOOT GROWTH; MIDDAY LEAF; CALIFORNIA; VINE; DRIP; SOIL; CONDUCTANCE; ROOT AB Vegetative growth and water relations of Thompson Seedless grapevines in response to applied water amounts at various fractions of measured grapevine ETc were quantified. Treatments ranged from no applied water up to 1.4 times the water used by vines growing in a weighing lysimeter. All treatments were irrigated at the same frequency as the vines in the lysimeter (whenever they used 2 mm of water), albeit at their respective fraction. Soil water content and midday leaf water potential (I-l) were measured routinely in four of the irrigation treatments across years. The amount of water depleted in the soil profile ranged from 190 mm for the 0.2 treatment in 1993 to no water depletion for the 1.4 treatment in 1992. The irrigation treatments significantly affected midday I-l, total shoot length, leaf area per vine, pruning weights and trunk diameter; as applied water decreased so did vegetative growth. Pruning weights were a linear function of the seasonal, mean midday I-l across growing seasons. The application of water amounts in excess of evapotranspiration negatively affected vegetative growth some of the years. A companion paper will demonstrate that over-irrigation can negatively affect reproductive growth of this grape cultivar due to excess vegetative growth. C1 [Williams, L. E.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Grimes, D. W.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Williams, L. E.; Grimes, D. W.] Kearney Agr Ctr, Parlier, CA 93648 USA. [Phene, C. J.] USDA ARS, Water Management Res Lab, Parlier, CA 93648 USA. RP Williams, LE (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM williams@uckac.edu FU American Vineyard Foundation; California Raisin Advisory Board FX We thank Peter Biscay, Weigang Yang, Nona Ebisuda and Paul Wiley for their technical assistance and R. M. Mead and D. A. Clark for their assistance with the weighing lysimeter. We also thank Dr. A. Patakas for his editorial comments. The research was funded in part by grants from the American Vineyard Foundation and the California Raisin Advisory Board to L. E. Williams. Mention of trade names or proprietary products is for the convenience of the reader only and does not constitute endorsement or preferential treatment by the University of California or USDA/ARS. NR 39 TC 26 Z9 26 U1 4 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAR PY 2010 VL 28 IS 3 BP 221 EP 232 DI 10.1007/s00271-009-0171-2 PG 12 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 551JX UT WOS:000274205300003 ER PT J AU Williams, LE Grimes, DW Phene, CJ AF Williams, Larry E. Grimes, D. W. Phene, C. J. TI The effects of applied water at various fractions of measured evapotranspiration on reproductive growth and water productivity of Thompson Seedless grapevines SO IRRIGATION SCIENCE LA English DT Article ID VITIS-VINIFERA L; DEFICIT IRRIGATION; WINE QUALITY; YIELD COMPONENTS; DRIP IRRIGATION; BUD NECROSIS; CROP LEVEL; VINEYARD; STRESS; FRUITFULNESS AB The reproductive growth and water productivity (WP(b)) of Thompson Seedless grapevines were measured as a function of applied water amounts at various fractions of measured grapevine ET(c) for a total of eight irrigation treatments. Shoots were harvested numerous times during the growing season to calculate water productivity. Berry weight was maximized at the 0.6-0.8 applied water treatments across years. As applied water amounts increased soluble solids decreased. Berry weight measured at veraison and harvest was a linear function of the mean midday leaf water potential measured between anthesis and veraison and anthesis and harvest, respectively. As applied water amounts increased up to the 0.6-0.8 irrigation treatments there was a significant linear increase in yield. Yields at greater applied water amounts either leveled off or decreased. The reduction in yield on either side of the yearly maximum was due to fewer numbers of clusters per vine. Maximum yield occurred at an ET(c) ranging from 550 to 700 mm. Yield per unit applied water and WP(b) increased as applied water decreased. The results from this study demonstrated that Thompson Seedless grapevines can be deficit irrigated, increasing water use efficiency while maximizing yields. C1 [Williams, Larry E.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Grimes, D. W.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Williams, Larry E.; Grimes, D. W.] Kearney Agr Ctr, Parlier, CA 93648 USA. [Phene, C. J.] USDA ARS, Water Management Res Lab, Parlier, CA 93648 USA. RP Williams, LE (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM williams@uckac.edu FU American Vineyard Foundation; California Raisin Advisory Board FX We thank Peter Biscay, Weigang Yang, Nona Ebisuda and Paul Wiley for their technical assistance and R. M. Mead and D. A. Clark for their assistance with the weighing lysimeter. We also thank Dr. A. Patakas for his editorial comments. The research was funded in part by grants from the American Vineyard Foundation and the California Raisin Advisory Board to L. E. Williams. Mention of trade names or proprietary products is for the convenience of the reader only and does not constitute endorsement or preferential treatment by the University of California or USDA/ARS. NR 36 TC 33 Z9 34 U1 1 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 J9 IRRIGATION SCI JI Irrig. Sci. PD MAR PY 2010 VL 28 IS 3 BP 233 EP 243 DI 10.1007/s00271-009-0173-0 PG 11 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 551JX UT WOS:000274205300004 ER PT J AU Mall, D Carll, C Daisey, G Golownia, R Knaebe, M Nebelsick, A Springate, R Williams, S AF Mall, Doug Carll, Charles Daisey, George Golownia, Robert Knaebe, Mark Nebelsick, Arnie Springate, Robert Williams, Sam TI CAULKING COMPOUNDS AND SEALANTS SO JCT COATINGSTECH LA English DT Article AB Caulks and sealants are widely used in building construction and renovation to fill gaps or holes in surfaces or seal joints between surfaces. These products are available in different chemistries and a range of qualities. When used properly according to manufacturers' instruction and with attention to workmanship, caulks and sealants can provide substantial benefits. RP Carll, C (reprint author), US Forest Serv, Durabil & Wood Protect, Res Forest Prod Technologist, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM ccarll@fs.fed.us NR 8 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION SOC COATINGS TECHNOLOGY PI BLUE BELL PA 527 PLYMOUTH RD, STE 415, BLUE BELL, PA 19462 USA SN 1547-0083 J9 JCT COATINGSTECH JI JCT Coatingstech PD MAR PY 2010 VL 7 IS 3 BP 40 EP + PG 8 WC Chemistry, Applied; Materials Science, Coatings & Films SC Chemistry; Materials Science GA 571GO UT WOS:000275740000007 ER PT J AU Burke, E Slavik, N Bonura, T Connelly, D Faris, T Nebelsick, A Stuart, B Williams, S Wledenhoeft, AC AF Burke, Edward Slavik, Norm Bonura, Tony Connelly, Dennis Faris, Tom Nebelsick, Arnie Stuart, Brent Williams, Sam Wledenhoeft, Alex C. TI UNDERSTANDING EXTRACTIVE BLEED WOOD EXTRACTIVES: DISTRIBUTION, PROPERTIES AND CLASSES SO JCT COATINGSTECH LA English DT Article AB Color, odor, and natural durability of heartwood are characteristics imparted by a class of chemicals in wood known as extractives. Water-soluble extractives (WSE) and solvent-soluble extractives (SSE) can cause premature finish failure on wood. Finish formulations have been developed that are effective at blocking stains resulting from WSE from coming to the surface of the finish. Recommendations are given to help prevent extractive bleed. C1 [Williams, Sam; Wledenhoeft, Alex C.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Wledenhoeft, AC (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM awiedenhoeft@fs.fed.us NR 4 TC 1 Z9 1 U1 1 U2 1 PU FEDERATION SOC COATINGS TECHNOLOGY PI BLUE BELL PA 527 PLYMOUTH RD, STE 415, BLUE BELL, PA 19462 USA SN 1547-0083 J9 JCT COATINGSTECH JI JCT Coatingstech PD MAR PY 2010 VL 7 IS 3 BP 48 EP + PG 7 WC Chemistry, Applied; Materials Science, Coatings & Films SC Chemistry; Materials Science GA 571GO UT WOS:000275740000008 ER PT J AU Butte, NF Nguyen, TT AF Butte, Nancy F. Nguyen, Tuan Thanh TI Is obesity an emerging problem in Brazilian children and adolescents? SO JORNAL DE PEDIATRIA LA English DT Editorial Material ID CORONARY-HEART-DISEASE; BODY-MASS INDEX; RISK-FACTORS; UNDERWEIGHT; OVERWEIGHT; TRENDS C1 [Butte, Nancy F.; Nguyen, Tuan Thanh] USDA ARS, Dept Pediat, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. RP Butte, NF (reprint author), USDA ARS, Dept Pediat, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. EM nbutte@bcm.tmc.edu NR 12 TC 1 Z9 1 U1 0 U2 0 PU SOC BRASIL PEDIATRIA PI RIO DE JANEIRO, RJ PA RUA SANTA CLARA 292, RIO DE JANEIRO, RJ, CEP 22401-01, BRAZIL SN 0021-7557 J9 J PEDIAT-BRAZIL JI J. Pediatr. PD MAR-APR PY 2010 VL 86 IS 2 BP 91 EP 92 DI 10.2223/JPED.1998 PG 2 WC Pediatrics SC Pediatrics GA 592QG UT WOS:000277393800002 PM 20361127 ER PT J AU Snelling, WM Allan, MF Keele, JW Kuehn, LA McDaneld, T Smith, TPL Sonstegard, TS Thallman, RM Bennett, GL AF Snelling, W. M. Allan, M. F. Keele, J. W. Kuehn, L. A. McDaneld, T. Smith, T. P. L. Sonstegard, T. S. Thallman, R. M. Bennett, G. L. TI Genome-wide association study of growth in crossbred beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; genome-wide association study; growth; single nucleotide polymorphism ID QUANTITATIVE TRAIT LOCI; AFFECTING MILK-PRODUCTION; FALSE DISCOVERY RATE; HOLSTEIN CATTLE; DAIRY-CATTLE; POPULATION-STRUCTURE; CARCASS COMPOSITION; FUNCTIONAL TRAITS; YEARLING WEIGHT; COMPLEX TRAITS AB Chromosomal regions harboring variation affecting cattle birth weight and BW gain to 1 yr of age were identified by marker association using the highly parallel BovineSNP50 BeadChip (50K) assay composed of 54,001 individual SNP. Genotypes were obtained from progeny (F(1); 590 steers) and 2-, 3-, and 4-breed cross grandprogeny (F(1)(2) = F(1) x F(1); 1,306 steers and 707 females) of 150 AI sires representing 7 breeds (22 sires per breed; Angus, Charolais, Gelbvieh, Hereford, Limousin, Red Angus, and Simmental). Genotypes and birth, weaning, and yearling BW records were used in whole-genome association analyses to estimate effects of individual SNP on growth. Traits analyzed included growth component traits: birth weight (BWT), 205-d adjusted birth to weaning BW gain (WG), 160-d adjusted postweaning BW gain (PWG); cumulative traits: 205-d adjusted weaning weight (WW = BWT + WG) and 365-d adjusted yearling weight (YW = BWT + WG + PWG); and indexes of relative differences between postnatal growth and birth weight. Modeled fixed effects included additive effects of calf and dam SNP genotype, year-sex-management contemporary groups, and covariates for calf and dam breed composition and heterosis. Direct and maternal additive polygenic effects and maternal permanent environment effects were random. Missing genotypes, including 50K genotypes of most dams, were approximated with a single-locus BLUP procedure from pedigree relationships and known 50K genotypes. Various association criteria were applied: stringent tests to account for multiple testing but with limited power to detect associations with small effects, and relaxed nominal P that may detect SNP associated with small effects but include excessive false positive associations. Genomic locations of the 231 SNP meeting stringent criteria generally coincided with described previously QTL affecting growth traits. The 12,425 SNP satisfying relaxed tests were located throughout the genome. Most SNP associated with BWT and postnatal growth affected components in the same direction, although detection of SNP associated with one component independent of others presents a possible opportunity for SNP-assisted selection to increase postnatal growth relative to BWT. C1 [Snelling, W. M.; Allan, M. F.; Keele, J. W.; Kuehn, L. A.; McDaneld, T.; Smith, T. P. L.; Thallman, R. M.; Bennett, G. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Sonstegard, T. S.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. RP Snelling, WM (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM warren.snelling@ars.usda.gov RI Keele, John/A-5846-2009 NR 54 TC 76 Z9 81 U1 0 U2 13 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 837 EP 848 DI 10.2527/jas.2009-2257 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600003 PM 19966163 ER PT J AU Hsu, WL Johnson, RK Van Vleck, LD AF Hsu, W. L. Johnson, R. K. Van Vleck, L. D. TI Effect of pen mates on growth, backfat depth, and longissimus muscle area of swine SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE backfat; competition; growth; longissimus muscle area; pen-mate effect; swine ID RESTRICTED MAXIMUM-LIKELIHOOD; NUMERATOR RELATIONSHIP MATRIX; LITTER SIZE SELECTION; AVERAGE DAILY GAIN; VARIANCE-COMPONENTS; MULTILEVEL SELECTION; GENETIC-PARAMETERS; CROSSBRED LITTERS; BREEDING VALUES; 19 GENERATIONS AB Records on final BW (kg), backfat depth (cm), and LM area (cm(2)) of pigs from a University of Nebraska Large White/Landrace composite population were analyzed to estimate the effects of pen mates. Measurements were at approximately 180 d of age for 3,524 pigs in 351 pens (9 to 11 pigs per pen) farrowed from 1999 to 2005. The area of each pen was 8.13 m(2). The full model (M1) included the fixed effects of contemporary group, sex, line, and the covariates of age and inbreeding coefficient, and included random direct genetic, genetic pen-mate, permanent environmental, pen, litter, and residual effects. A derivative-free algorithm was used to obtain REML estimates of variance components for final BW adjusted to 180 d of age with M1 and 7 reduced models, and with 4 reduced models for the carcass traits. For final BW, likelihood ratio tests showed that M1 did not fit the data better than model 2 (permanent environmental effect omitted from M1) or model 3 (pen omitted from M1). Model 2 was not significantly (P > 0.05) better than model 3, which shows that variance attributable to pen effects and permanent environmental effects cannot be separated. Large sampling variances of estimates of the pen component of variance for models with pen-mate effects also indicate an inability to separate pen effects from the effects of pen mates. When pen-mate genetic effects were not in the model, estimates of components of variance and the fit of the data were the same for models 4 (included both permanent environmental and pen effects), 6 (included pen effects), and 7 (included permanent environmental effects), which shows that including both pen and permanent environmental effects was no better than including one or the other. Models 4, 6, and 7 were significantly better than model 8, which did not include pen-mate effects and pen effects, implying that pen effects are important. The estimate of pen variance with model 2 was approximately (number of pen mates - 1) times the estimate of variance of pen-mate permanent environmental effects with model 3. Patterns of estimates of variance components with models 2, 5, 6, and 8 for backfat depth and LM area were similar to those for final BW. Estimates of direct genetic variance and phenotypic variance were similar for all models. Estimates of heritability for direct genetic effects were approximately 0.40 for final BW, 0.45 for backfat depth, and 0.27 for LM area. Estimates of heritability for pen-mate genetic effects were 0.001 for the 3 traits for models including either pen or permanent environmental effects. Under the management conditions for this experiment, the conclusion is that the model for genetic evaluation should include litter effects and either pen effects or pen-mate permanent environmental effects and possibly genetic pen-mate effects, in general agreement with the results of studies of different populations at other locations. C1 [Hsu, W. L.; Johnson, R. K.; Van Vleck, L. D.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Van Vleck, L. D.] ARS, US Meat Anim Res Ctr, USDA, Lincoln, NE 68583 USA. RP Van Vleck, LD (reprint author), Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. EM lvanvleck@unlnotes.unl.edu NR 36 TC 9 Z9 10 U1 0 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 895 EP 902 DI 10.2527/jas.2009-1879 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600009 PM 19933426 ER PT J AU Geary, TW Ansotegui, RP MacNeil, MD Roberts, AJ Waterman, RC AF Geary, T. W. Ansotegui, R. P. MacNeil, M. D. Roberts, A. J. Waterman, R. C. TI Effects of flunixin meglumine on pregnancy establishment in beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; flunixin meglumine; pregnancy establishment ID HUMAN CHORIONIC-GONADOTROPIN; ARTIFICIAL-INSEMINATION; EMBRYO-TRANSFER; PLASMA-CONCENTRATIONS; CONCEPTION RATES; CORPUS-LUTEUM; COWS; PROGESTERONE; HEIFERS; PROSTAGLANDIN-F2-ALPHA AB The objective of this research was to determine effects of a single injection of the PG synthesis inhibitor flunixin meglumine (FM; 1.1 mg/kg of BW, intramuscularly) approximately 13 d (range 10 to 15 d) after AI on pregnancy establishment. Three experiments were conducted using estrus-synchronized heifers and cows. Technicians and AI sires were equally represented across treatments within locations and experiments. Bulls were introduced on the day of FM treatment. Pregnancy to AI was diagnosed 28 to 50 d after AI using ultrasonography. In Exp. 1, beef heifers (n = 1,221) were divided within 5 locations to receive FM or no further treatment (control). At insemination, heifers were divided into 2 similar pastures or pens, and approximately 13 d later, 1 group of heifers within each location was processed through an animal handling facility to administer FM treatment. There was no location x treatment interaction (P = 0.62) on AI pregnancy rates, so data were pooled. Pregnancy rates to AI were reduced (P = 0.02) among heifers receiving the FM treatment procedure (66%) compared with control heifers (72%). In Exp. 2, suckled beef cows (n = 719) were assigned within 2 locations to receive FM or no further treatment (control) approximately 13 d after AI. At insemination, control and FM cows were divided into separate pastures, and only FM cows were handled after AI for the FM treatment procedure. There was no location x treatment interaction (P = 0.75), so data were pooled. Pregnancy rates to AI did not differ (P = 0.80) between FM (57%) and control cows (59%). In Exp 3, beef heifers (n = 247) and suckled beef cows (n = 335) from 1 location received no injection (control) or injection of FM approximately 13 d after AI when all cows and heifers were processed through a working facility. Pregnancy rates to AI were not different (P = 0.37) between FM (45%) and control (42%) cows or between FM (56%) and control (55%) heifers. We conclude FM administration at 1.1 mg/kg of BW approximately 13 d after AI did not improve pregnancy establishment in beef cows and heifers and that the effects of handling heifers at this time may decrease pregnancy establishment. C1 [Geary, T. W.; MacNeil, M. D.; Roberts, A. J.; Waterman, R. C.] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Ansotegui, R. P.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USA. RP Geary, TW (reprint author), ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM tom.geary@ars.usda.gov RI MacNeil, Michael/A-6772-2009 FU National Association of Animal Breeders (Columbia, MO); American Simmental Association (Bozeman, MT); Schering Plough (Kenilworth, NJ); Pfizer Animal Health (Kalamazoo, MI); Teva Animal Health (Saint Joseph, MO) FX Mention of a proprietary product does not constitute a guarantee or warranty of the product by USDA, Montana Agriculture Experiment Station, or the authors and does not imply its approval to the exclusion of other products that may also be suitable. The USDA, ARS, Northern Plains Area, is an equal opportunity/affirmative action employer. All agency services are available without discrimination. The authors acknowledge the National Association of Animal Breeders (Columbia, MO), American Simmental Association (Bozeman, MT), Schering Plough (Kenilworth, NJ), Pfizer Animal Health (Kalamazoo, MI), and Teva Animal Health (Saint Joseph, MO) for their support of this research and Brent Thompson (DVM) for his assistance with treatment administration. NR 38 TC 11 Z9 11 U1 0 U2 6 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 943 EP 949 DI 10.2527/jas.2009-2087 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600015 PM 19933424 ER PT J AU Lekatz, LA Caton, JS Taylor, JB Reynolds, LP Redmer, DA Vonnahme, KA AF Lekatz, L. A. Caton, J. S. Taylor, J. B. Reynolds, L. P. Redmer, D. A. Vonnahme, K. A. TI Maternal selenium supplementation and timing of nutrient restriction in pregnant sheep: Effects on maternal endocrine status and placental characteristics SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE angiogenic factor; maternal nutrition; placenta; selenium; sheep ID VISCERAL ORGAN MASS; ANGIOGENIC FACTOR EXPRESSION; FETAL BODY-WEIGHT; DIETARY RESTRICTION; EWE LAMBS; VASCULAR DEVELOPMENT; OXIDATIVE STRESS; INTESTINAL CELLULARITY; GLUTATHIONE-PEROXIDASE; JEJUNAL VASCULARITY AB To determine the effects of maternal Se intake and plane of nutrition during midgestation, late gestation, or both on hormone and metabolite concentrations in the dam and on placental characteristics, pregnant ewe lambs (n = 64) were assigned to 1 of 8 treatments arranged in a 2 x 2 x 2 factorial array: Se level [initiated at breeding; adequate (3.05 mu g/kg of BW) or high (70.4 mu g/kg of BW)] and nutritional level [100% (control) or 60% (restricted) of NRC recommendations] fed at different times of gestation [d 50 to 90 (midgestation) or d 91 to 130 (late gestation)]. The control ewes had a greater (P = 0.01) percentage change in BW from d 50 than restricted ewes during both mid- and late gestation. Although blood urea N was not affected by either Se or nutritional level, restricted ewes had greater (P = 0.01) concentrations of circulating Se on d 66, 78, 106, 120, and 130 of gestation compared with control ewes. Both Se and timing of the nutritional level affected circulating progesterone; however, only nutritional level affected thyroxine and triiodothyronine concentrations in the dam. Nutrient restriction during late gestation decreased (P = 0.01) fetal BW and fetal fluid weight compared with the control ewes (3.75 vs. 4.13 +/- 0.10 kg and 1.61 vs. 2.11 +/- 0.11 kg). Although neither Se nor nutritional level affected (P = 0.1) placental, caruncular, or cotyledonary weights, cotyledonary cellular proliferation was decreased (P < 0.05) in ewes receiving a high concentration of Se compared with those receiving adequate Se. In addition, either Se or nutritional level affected vascular endothelial growth factor (VEGFA), VEGFA-receptor 1, VEGFA-receptor 2, and NO synthase mRNA abundance in the cotyledonary tissue. In the caruncular tissue, either Se or nutritional level affected VEGFA-receptor 1, placental growth factor, and NO synthase mRNA abundance. Selenium supplementation and the duration or timing of nutrient restriction appear to influence the endocrine and metabolic status of the ewe, which may influence nutrient transport and placental function. C1 [Lekatz, L. A.; Caton, J. S.; Reynolds, L. P.; Redmer, D. A.; Vonnahme, K. A.] N Dakota State Univ, Ctr Nutr & Pregnancy, Dept Anim Sci, Fargo, ND 58108 USA. [Taylor, J. B.] ARS, USDA, US Sheep Expt Stn, Dubois, ID 83423 USA. RP Vonnahme, KA (reprint author), N Dakota State Univ, Ctr Nutr & Pregnancy, Dept Anim Sci, Fargo, ND 58108 USA. EM Kim.Vonnahme@ndsu.edu RI Reynolds, Lawrence/I-5267-2015 OI Reynolds, Lawrence/0000-0002-6838-7809 FU USDA Cooperative State Research, Education, and Extension Service [2003-35206-13621, 2005-35206-15281]; North Dakota State University FX This project was partially supported by National Research Initiative Competitive Grants 2003-35206-13621 and 2005-35206-15281 from the USDA Cooperative State Research, Education, and Extension Service. Gratitude is expressed to employees of the North Dakota State University Animal Nutrition and Physiology Center and the Reproductive Physiology and Ruminant Nutrition Laboratories for their contributions to this project. NR 67 TC 25 Z9 25 U1 1 U2 10 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 955 EP 971 DI 10.2527/jas.2009-2152 PG 17 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600017 PM 19933425 ER PT J AU Freetly, HC Ferrell, CL Archibeque, S AF Freetly, H. C. Ferrell, C. L. Archibeque, S. TI Net flux of amino acids across the portal-drained viscera and liver of the ewe during abomasal infusion of protein and glucose SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE amino acid; intestine; liver; sheep ID FATTY-ACIDS; GASTROINTESTINAL-TRACT; MESENTERIC VEIN; PREGNANT EWES; METABOLISM; SHEEP; ABSORPTION; OXIDATION; LACTATE; STARCH AB The objective of the study was to measure net AA flux rates across the portal-drained viscera (PDV) and liver in the presence and absence of abomasal glucose infusion. Decreasing the fraction of AA metabolized by the mucosal cells may increase the fraction of AA being released into the blood. A potential mechanism to reduce AA catabolism by mucosal cells is to provide an alternative source of energy. We hypothesized that increasing glucose flow to the small intestine would increase net appearance of AA across the PDV. Eighteen mature ewes with sampling catheters were placed on study. The experimental design was a split-plot with a complete randomized design on the whole-plot and a Latin-square subplot with 5 periods and incremental levels of protein infusion. One-half of the ewes received abomasal glucose infusions (3.84 g/h), and all ewes received each of 5 protein abomasal infusion levels over 5 periods (0, 2.6, 5.2, 7.8, and 10.5 g/h). Net PDV release of isoleucine, leucine, methionine, phenylalanine, aspartate, glutamate, glutamine, proline, serine, and tyrosine increased linearly with increased protein infusion, and net PDV release of histidine, lysine, threonine, valine, alanine, and glycine did not differ with protein infusion. Net hepatic glucose release decreased with glucose infusion. With the exception of histidine, phenylalanine, and valine, net hepatic AA uptake increased linearly with increased delivery of AA to the liver. Glucose infusion increased the hepatic lysine and valine uptake and decreased phenylalanine uptake. Based on the observations in the current study, we reject our hypothesis that glucose can spare AA metabolism by PDV tissue. Our findings suggest that hepatic gluconeogenesis can be increased in the presence of increased AA delivery to the liver and that hepatic gluconeogenesis can be decreased with increased absorption of dietary glucose. Our findings support the concept that for most AA, hepatic transport of AA can be described by mass action kinetics; however, the rates of hepatic uptake of specific AA are upregulated directly or indirectly by elevated glucose. C1 [Freetly, H. C.; Ferrell, C. L.; Archibeque, S.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Freetly, HC (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Harvey.freetly@ars.usda.gov NR 26 TC 5 Z9 5 U1 0 U2 6 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 1093 EP 1107 DI 10.2527/jas.2009-2260 PG 15 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600030 PM 19966174 ER PT J AU King, DA Shackelford, SD Kuehn, LA Kemp, CM Rodriguez, AB Thallman, RM Wheeler, TL AF King, D. A. Shackelford, S. D. Kuehn, L. A. Kemp, C. M. Rodriguez, A. B. Thallman, R. M. Wheeler, T. L. TI Contribution of genetic influences to animal-to-animal variation in myoglobin content and beef lean color stability SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef; breed; color stability; genetics; heritability ID METMYOGLOBIN-REDUCING-ACTIVITY; INJECTION-ENHANCED BEEF; MUSCLE-FIBER TYPES; BOVINE MUSCLES; CARCASS COMPOSITION; PALATABILITY TRAITS; LONGISSIMUS MUSCLE; MEAT COLOR; CATTLE; LACTATE AB Longissimus thoracis steaks from steers (n = 464) with 0 to 50% inheritance of Angus, Charolais, Gelbvieh, Hereford, Limousin, Red Angus, and Simmental were evaluated during 6 d of display to assess genetic contributions to color stability. Color space values [CIE L* (lightness), a* (redness), b* (yellowness)], chroma, color change (Delta E), and surface metmyoglobin (K/S 572/525) were determined on d 0 and 6 of display. Myoglobin concentration was highly heritable (0.85), but ultimate pH was weakly heritable (0.06). Day 0 L* values were moderately heritable (0.24). Variation in metmyoglobin, L*, and Delta E on d 6 was moderately explained by genetic factors (41, 40, and 29%, respectively). Change during display was moderately heritable for a* (0.31), b* (0.23), chroma (0.35), and surface metmyoglobin (0.29). At the start of display, Angus steaks had greater (P < 0.05) L* values than those from all breeds except Charolais. On d 6, Angus steaks had greater (P < 0.05) L* (50.0) values than Gelbvieh, Hereford, and Simmental steaks (46.1, 44.0, and 44.5, respectively). Day 0 values for a*, b*, chroma, and Delta E were not affected by breed (P > 0.05). On d 6, a* values were greater (P < 0.05) for Charolais and Limousin steaks (31.1 and 30.5) than Angus, Hereford, and Red Angus steaks (27.4, 27.7, and 26.3, respectively). Thus, a* changed less (P < 0.05) in Charolais and Limousin steaks (1.8 and 2.6, respectively) vs. steaks from other breeds. Day 6 b* values were greater (P < 0.05) in Charolais (24.5) and Limousin steaks (24.0) vs. Gelbvieh (22.2), Hereford (21.9), and Red Angus steaks (21.4). Thus, b* values changed less (P < 0.05) in Charolais and Limousin steaks (1.5 and 1.7, respectively) than in Angus, Gelbvieh, Hereford, and Red Angus steaks (4.3, 3.8, 4.4, and 5.1, respectively). After 6 d of display, Charolais and Limousin steaks had greater chroma (P < 0.05; 39.5 and 38.8, respectively) compared with Angus, Hereford, and Red Angus steaks (35.4, 35.3, and 33.9, respectively). Less (P < 0.05) change in chroma occurred for Charolais and Limousin (2.1 and 2.8, respectively) than in Angus, Gelbvieh, Hereford, and Red Angus steaks (7.1, 6.6, 7.4, and 9.0, respectively). Myoglobin concentration was less for Charolais and Limousin (P < 0.05; 2.77 and 2.72, respectively) compared with Gelbvieh, Red Angus, and Simmental steaks (3.62, 3.43, and 3.71, respectively). Breeds did not differ in pH (P > 0.05). These data suggest Charolais- and Limousin-carcasses produced steaks with greater lean color stability than Angus, Hereford, and Red Angus carcasses. Furthermore, these findings suggest that genetics contribute substantially to animal-to-animal variation in lean color, particularly in maintaining color. C1 [King, D. A.; Shackelford, S. D.; Kuehn, L. A.; Kemp, C. M.; Rodriguez, A. B.; Thallman, R. M.; Wheeler, T. L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP King, DA (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM andy.king@ars.usda.gov FU Fundacion Caja Madrid (Spain) FX Mention of trade names, proprietary products, or specified equipment does not constitute a guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that may be suitable. The authors are grateful to Patty Beska, Kathy Mihm, Kristen Ostdiek, and Pat Tammen of the US Meat Animal Research Center for their assistance in the execution of this experiment and to Marilyn Bierman of the US Meat Animal Research Center for her secretarial assistance. A. B. Rodriguez thanks the Fundacion Caja Madrid (Spain) for financial assistance. NR 39 TC 24 Z9 25 U1 2 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 1160 EP 1167 DI 10.2527/jas.2009-2544 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600035 PM 19966159 ER PT J AU Poletto, R Meisel, RL Richert, BT Cheng, HW Marchant-Forde, JN AF Poletto, R. Meisel, R. L. Richert, B. T. Cheng, H. W. Marchant-Forde, J. N. TI Behavior and peripheral amine concentrations in relation to ractopamine feeding, sex, and social rank of finishing pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE aggression; amine; ractopamine; sex; social rank; swine ID BLOOD-BRAIN-BARRIER; GROWTH-PERFORMANCE; DOMESTIC PIGS; CARCASS MERIT; GROWING PIGS; STRESS; PLASMA; CATECHOLAMINES; AGGRESSIVENESS; GENOTYPE AB Aggression can impair productivity and well-being. The association between aggression in finishing pigs and the feed additive ractopamine (RAC), a beta-adrenoreceptor agonist, is unknown and warrants further investigation. Our goal was to examine behavioral activity, including aggression, in the home pen and concentrations of peripheral amines in barrows and gilts, taking into account diet (RAC) and social rank. Sixty-four finishing pigs, housed in pens of 4 by sex, were fed either a control (CTL) or RAC-added (5 mg/kg for 2 wk plus 10 mg/kg for another 2 wk) diet. The top dominant and bottom subordinate pigs in each pen were determined at mixing (2 wk pretrial). The behavior of all pigs was recorded continuously during the pretrial week (baseline) and for the following 4 wk. These behavioral data were used to evaluate home pen aggression, including the number of agonistic interactions (AINX) and constituent aggressive actions, during a 3-h period (0800 to 1100 h) once per week and their change in relation to the baseline. Time-budget behaviors and postures were analyzed over eight 24-h periods (2 d/wk) using 10-min instantaneous scan sampling that focused on only the dominant and subordinate pigs in each pen. These 2 pigs were also subjected to blood collection once per week during the trial to determine concentrations of dopamine, norepinephrine, epinephrine, and serotonin (5-HT) using HPLC. Gilts performed more bites and total actions per AINX than barrows, and RAC-fed gilts increased bites and pursuits, whereas these behaviors decreased compared with baseline values in all other subgroups (P < 0.05). Gilts fed RAC increased the total number actions per AINX, whereas the occurrence of AINX decreased for all subgroups (P < 0.01). Overall, RAC-fed pigs were more behaviorally active, spending more time alert, bar biting, and sham chewing compared with CTL pigs (P < 0.05). The dominant RAC-fed pigs tended to have the greatest norepinephrine concentrations among the tested subgroups (P = 0.08). Dominant barrows had greater epinephrine concentrations than subordinate barrows (P < 0.05). The RAC-fed gilts tended to have lesser 5-HT concentrations than CTL gilts (P = 0.08), whereas concentrations were similar in barrows (P > 0.10). Greater activity and the increase in oral-related behaviors observed in RAC-fed pigs may be mediated by the increase in arousal caused by RAC. Intensified aggression in gilts, especially when fed RAC, may be linked to reduced central 5-HT and greater noradrenergic activity, and further research on brain neurotransmitters in gilts is needed. C1 [Poletto, R.; Cheng, H. W.; Marchant-Forde, J. N.] ARS, USDA, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Poletto, R.; Richert, B. T.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Meisel, R. L.] Purdue Univ, Dept Psychol Sci, W Lafayette, IN 47906 USA. RP Marchant-Forde, JN (reprint author), ARS, USDA, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM Jeremy.Marchant-Forde@ars.usda.gov RI Marchant-Forde, Jeremy/A-7616-2009 OI Marchant-Forde, Jeremy/0000-0002-5287-2914 NR 37 TC 16 Z9 16 U1 0 U2 13 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 1184 EP 1194 DI 10.2527/jas.2008-1576 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600037 PM 19933435 ER PT J AU Eicher, SD Wesley, IV Sharma, VK Johnson, TR AF Eicher, S. D. Wesley, I. V. Sharma, V. K. Johnson, T. R. TI Yeast cell-wall products containing beta-glucan plus ascorbic acid affect neonatal Bos taurus calf leukocytes and growth after a transport stressor SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; beta-glucan; immunity; transportation; vitamin C; yeast cell wall ID ESCHERICHIA-COLI O157-H7; VITAMIN-C; FLUOROGENIC PCR; CALVES; CATTLE; BEEF; AGE AB The objectives were to ascertain whether a yeast cell-wall derivative that was 1.8% beta-glucan in combination with ascorbyl-2-polyphosphate could improve innate immunity and mediate transportation stress in neonatal calves, and to compare the 1.8% beta-glucan yeast cell-wall derivative with a more purified yeast cell-wall derivative (70% beta-glucan). Treatments were 1) an unsupplemented control (CNT); 2) 113 g of a 1.8% (approximately 2%) beta-glucan derivative of yeast cell walls plus 250 mg of L-ascorbic acid phosphate (BG2); or 3) 150 mg of a purified beta-glucan fraction from yeast cell walls (approximately 70% beta-glucan) plus 250 mg/feeding of L-ascorbic acid phosphate (BG70). Calves (n = 39) were transported for 4 h, placed in outdoor hutches, and randomly assigned to treatments. Treatments (mixed with a milk replacer) were individually fed twice daily for 28 d. Calves were offered calf starter, free choice, throughout the study. Weekly starter intake and BW were measured, and fecal samples were collected for Salmonella Typhimurium and Escherichia coli O157:H7 PCR analysis. Blood was collected immediately before transport (d 0) and on d 3, 7, 10, 14, 21, and 28 after transport. Starter intake and DMI were less (P < 0.05) at d 28 for the BG2 and BG70 treatments compared with the CNT treatment. Hematocrit percentages increased (P = 0.002) throughout the experiment. White blood cell counts (treatment x time interaction, P = 0.066) were less for the calves supplemented with BG70 than for those supplemented with BG2 (P = 0.01) or for CNT calves (P = 0.04) on d 28. Granulocyte counts changed (P = 0.04) throughout the experiment. A trend (P = 0.077) for a treatment x time interaction was detected for peripheral blood mononuclear cell counts (PBMC). Counts of PBMC were greater (P = 0.006) for the BG2 treatment compared with the CNT treatment on d 3. Calves given the BG70 supplement had fewer PBMC than those given the BG2 supplement on d 21 (P = 0.03) and 28 (P = 0.05). Fibrinogen concentrations were affected only by time (P = 0.002). Time effects were detected for phagocytosis (P = 0.005), oxidative burst (P < 0.001), expression of cluster of differentiation 18 (P = 0.001), and increased cluster of differentiation 18 (P = 0.006). Phagocytosis was less (P = 0.05) for calves in the BG70 group than for those in the CNT group. Percentage of calves positive for E. coli O157:H7 was greatest (P <= 0.05) for those in the BG2 group on d 7 compared with those in the other treatments. The BG2 and BG70 supplements both increased feed intake, but only the BG2 supplement increased E. coli shedding on d 7, and the BG2 and BG70 supplements varied in modulating immune functions, indicating differences in yeast extract function. C1 [Eicher, S. D.] ARS, USDA, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Wesley, I. V.; Sharma, V. K.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Johnson, T. R.] Purdue Univ, W Lafayette, IN 47907 USA. RP Eicher, SD (reprint author), ARS, USDA, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM Susan.Eicher@ars.usda.gov NR 27 TC 5 Z9 7 U1 1 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA 2441 VILLAGE GREEN PLACE, CHAMPAIGN, IL 61822 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2010 VL 88 IS 3 BP 1195 EP 1203 DI 10.2527/jas.2008-1669 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 561TK UT WOS:000275001600038 PM 19933428 ER PT J AU Lehotay, SJ Mastovska, K Lightfield, AR Gates, RA AF Lehotay, Steven J. Mastovska, Katerina Lightfield, Alan R. Gates, Robert A. TI Multi-Analyst, Multi-Matrix Performance of the QuEChERS Approach for Pesticide Residues in Foods and Feeds Using HPLC/MS/MS Analysis with Different Calibration Techniques SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; MULTIRESIDUE METHOD; MATRIX; GAS; VEGETABLES; PRODUCE; FRUITS AB Three different calibration approaches were applied in HPLC/MS/MS using electrospray ionization for the determination of 14 diverse pesticide residues at different levels in a variety of food matrixes. This study was conducted as part of a 4 day training course for 17 chemists to learn the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) approach to pesticide residue analysis in foods. The analysts were divided into four different teams for the analysis of 12 different matrixes (strawberries, plums, carrots, green peppers, milk, molasses, alfalfa oats, corn silage, dry pet food, soybean, almonds, and foliage). The acetate-buffered QuEChERS protocol gave excellent results in the spiked samples for all matrixes tested. The HPLC/MS/MS calibration techniques consisted of external standardization in solvent solutions, matrix-matching, and the echo-peak technique. Peak areas were normalized to an internal standard in all three approaches. Matrix effects were observed with the corn silage, carrot, and foliage extracts, but they were minimal or nonexistent in the other matrixes. Matrix-matching best compensated for matrix effects, but has logistical difficulties in real-world application and required extra sample preparation compared to the other approaches. The echo-peak technique reduced matrix effects but did not eliminate them. C1 [Lehotay, Steven J.; Mastovska, Katerina; Lightfield, Alan R.; Gates, Robert A.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Ln, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov NR 23 TC 37 Z9 42 U1 4 U2 37 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 J9 J AOAC INT JI J. AOAC Int. PD MAR-APR PY 2010 VL 93 IS 2 BP 355 EP 367 PG 13 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA 589FR UT WOS:000277132800002 PM 20480880 ER PT J AU Miller, RS Miller, WG Behringer, M Hariharan, H Matthew, V Oyarzabal, OA AF Miller, R. S. Miller, W. G. Behringer, M. Hariharan, H. Matthew, V. Oyarzabal, O. A. TI DNA identification and characterization of Campylobacter jejuni and Campylobacter coli isolated from caecal samples of chickens in Grenada SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Campylobacter; chicken; flaA-RFLP; Grenada; MLST; PFGE ID FIELD GEL-ELECTROPHORESIS; PCR; STRAINS; DIVERSITY; BROILER; GENE; LARI; ENUMERATION; RESISTANCE; PATTERNS AB Aims: To speciate Campylobacter strains from the caeca of chickens in Grenada using PCR and to evaluate DNA-based typing methods for the characterization of these isolates. Methods and Results: Isolates were speciated with two multiplex PCR assays and were typed with flaA-RFLP, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results confirmed that Campylobacter coli strains were more predominant than Campylobacter jejuni strains. From 56 isolates, 18 were misidentified using biochemical tests. PFGE typing gave the highest discriminatory power among the methods used (Simpson's index of diversity, D = 0 center dot 9061). However, the combination of flaA-RFLP, PFGE and MLST results gave the highest discrimination for subtyping of these isolates (D = 0 center dot 9857). A band position tolerance of 4% in BioNumerics was the most appropriate for the analysis of this database. MLST profiles were generally concordant with PFGE and/or flaA-RFLP types. Several isolates exhibited new MLST sequence types (STs), and 43 of the 49 Camp. coli strains belonged to the ST-828 clonal complex. Conclusions: Campylobacter coli was the most prevalent species isolated from broilers and layers in Grenada, and a combination of restriction and sequence methods was most appropriate for the typing of Camp. coli isolates. Campylobacter coli STs clustered with described poultry-associated Camp. coli STs by phylogenetic analysis. Significance and Impact of the Study: Further studies to understand the predominance of Camp. coli within Campylobacter spp. from chickens in Grenada may help elucidate the epidemiology of these pathogens in chickens. C1 [Miller, R. S.; Behringer, M.; Oyarzabal, O. A.] Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA. [Miller, W. G.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. [Hariharan, H.; Matthew, V.] St Georges Univ, Sch Vet Med, Dept Paraclin Studies, St Georges, Grenada. RP Oyarzabal, OA (reprint author), Auburn Univ, Dept Poultry Sci, 260 Lem Morrison Dr, Auburn, AL 36849 USA. EM oyarzoa@auburn.edu OI Oyarzabal, Omar/0000-0002-7863-2767 FU Wellcome Trust FX This publication made use of the Campylobacter jejuni Multi Locus Sequence Typing website (http://pubmlst.org/campylobacter/) developed by Keith Jolley and Man-Suen Chan and sited at the University of Oxford (Jolley et al., 2004, BMC Bioinformatics5, 86). The development of this site has been funded by the Wellcome Trust. NR 37 TC 16 Z9 17 U1 0 U2 3 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAR PY 2010 VL 108 IS 3 BP 1041 EP 1049 DI 10.1111/j.1365-2672.2009.04507.x PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 553TH UT WOS:000274389200031 PM 19735321 ER PT J AU Ordovas, JM Smith, CE AF Ordovas, Jose M. Smith, Caren E. TI PLIN1 gene: fat keeper and prevention switcher SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Editorial Material ID OBESITY RISK; PERILIPIN; POLYMORPHISM; ASSOCIATION; POPULATION; HAPLOTYPE; LOCUS; WOMEN C1 [Ordovas, Jose M.; Smith, Caren E.] Tufts Univ, JM USDA HNRCA, Nutr & Genom Lab, Boston, MA 02111 USA. RP Ordovas, JM (reprint author), Tufts Univ, JM USDA HNRCA, Nutr & Genom Lab, Boston, MA 02111 USA. OI Ordovas, Jose/0000-0002-7581-5680 FU NHLBI NIH HHS [HL-54776, R01 HL054776]; NIDDK NIH HHS [DK-075030, R01 DK075030] NR 10 TC 1 Z9 2 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD MAR PY 2010 VL 108 IS 3 BP 477 EP 478 DI 10.1152/japplphysiol.00036.2010 PG 2 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA 570JK UT WOS:000275670400004 PM 20075266 ER PT J AU Gardner, DR Panter, KE Stegelmeier, BL AF Gardner, Dale R. Panter, Kip E. Stegelmeier, Bryan L. TI Implication of agathic acid from Utah juniper bark as an abortifacient compound in cattle SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE agathic acid; cattle; abortion; poisonous plants ID PINE NEEDLE ABORTION; PONDEROSA PINE; ISOCUPRESSIC ACID; RANGE CATTLE; BEEF-CATTLE AB Freshly ground Utah juniper [Juniperus osteosperma (Torr.) Little] bark was given via gavage at a dosage of 2.3 kg per cow twice daily to three pregnant cows starting on day 255 of gestation. All three cows aborted the calves after 4, 5 and 6 days of treatment. A fourth cow was dosed with Utah juniper needles and this cow calved early on day 268 of gestation with complications consistent with pine needle abortion. Chemical analysis of Juniperus osteosperma bark identified the major diterpene acid as the labdane acid known as agathic acid. Agathic acid was measured in the bark at a concentration of 1.5% (dry weight basis). Analysis of sera samples obtained from treated cows found detectable quantities of agathic acid, dihydroagathic acid and tetrahydroagathic acid, which are known serum metabolites of the abortifacient compound isocupressic acid. Based on the high incidence of induced abortion and detection of known metabolites in affected animals, the labdane acid known as agathic acid is considered to be an abortifacient compound in late-term pregnant cattle. Published in 2009 by John Wiley & Sons, Ltd. C1 [Gardner, Dale R.; Panter, Kip E.; Stegelmeier, Bryan L.] ARS, USDA, PPRL, Logan, UT 84335 USA. RP Gardner, DR (reprint author), ARS, USDA, PPRL, 1150 E 1400 N, Logan, UT 84335 USA. EM dale.gardner@ars.usda.gov NR 15 TC 10 Z9 10 U1 0 U2 6 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0260-437X J9 J APPL TOXICOL JI J. Appl. Toxicol. PD MAR PY 2010 VL 30 IS 2 BP 115 EP 119 DI 10.1002/jat.1476 PG 5 WC Toxicology SC Toxicology GA 568JW UT WOS:000275519600002 PM 19757408 ER PT J AU Heffernan, KS Fahs, CA Ranadive, SM Patvardhan, EA AF Heffernan, Kevin S. Fahs, Christopher A. Ranadive, Sushant M. Patvardhan, Eshan A. TI L-Arginine as a Nutritional Prophylaxis Against Vascular Endothelial Dysfunction With Aging SO JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS LA English DT Review DE vascular; endothelial; inflammation; growth hormone ID ORAL L-ARGININE; CORONARY-ARTERY-DISEASE; GROWTH-FACTOR-I; NITRIC-OXIDE BIOAVAILABILITY; FLOW-MEDIATED DILATION; HEALTHY-YOUNG MEN; OXIDATIVE STRESS; ASYMMETRIC DIMETHYLARGININE; DEPENDENT VASODILATION; PLATELET-AGGREGATION AB With advancing age, peripheral conduit and resistance arteries lose the ability to effectively dilate owing to endothelial dysfunction. This vascular senescence contributes to increased risk of cardiovascular disease (CVD) with aging. L-arginine plays a role in numerous physiological processes including nitrogen detoxification, immunocompetence, growth hormone (GH) secretion, and insulin secretion. Recently, a considerable amount of attention has been placed on the ability of this amino acid to affect vascular endothelial function. The purpose of this review will be to examine the use of L-arginine as a novel nutritional strategy to potentially stave progression of vascular dysfunction with aging and CVD. Emphasis will be placed on the ability of L-arginine to modulate the vascular inflammatory and systemic hormonal milieu, which in turn may have a positive effect on vascular endothelial function. C1 [Heffernan, Kevin S.; Patvardhan, Eshan A.] Tufts Med Ctr, Div Cardiol, Dept Med, Mol Cardiol Res Inst, Boston, MA 02111 USA. [Heffernan, Kevin S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Medford, MA 02155 USA. [Fahs, Christopher A.; Ranadive, Sushant M.] Univ Illinois, Exercise & Cardiovasc Res Lab, Dept Kinesiol & Community Hlth, Champaign, IL 61820 USA. RP Heffernan, KS (reprint author), Tufts Med Ctr, Div Cardiol, Dept Med, Mol Cardiol Res Inst, 800 Washington St, Boston, MA 02111 USA. EM KHeffernan@TuftsMedicalCenter.org RI Ranadive, Sushant/J-7601-2013 FU NHLBI NIH HHS [T32 HL069770, T32 HL069770-06A1] NR 76 TC 19 Z9 20 U1 0 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1074-2484 J9 J CARDIOVASC PHARM T JI J. Cardiovasc. Pharmacol. Ther. PD MAR PY 2010 VL 15 IS 1 BP 17 EP 23 DI 10.1177/1074248409354599 PG 7 WC Cardiac & Cardiovascular Systems; Pharmacology & Pharmacy SC Cardiovascular System & Cardiology; Pharmacology & Pharmacy GA 552TI UT WOS:000274316100002 PM 20053922 ER PT J AU Smith, CM Liu, XM Wang, LJ Liu, X Chen, MS Starkey, S Bai, JF AF Smith, Charles Michael Liu, Xuming Wang, Liang J. Liu, Xiang Chen, Ming-Shun Starkey, Sharon Bai, Jianfa TI Aphid Feeding Activates Expression of a Transcriptome of Oxylipin-based Defense Signals in Wheat Involved in Resistance to Herbivory SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Diuraphis noxia; Insect; Microarray; Northern blot; Oxylipin signaling; Plant defense; Real-time polymerase chain reaction (PCR); Russian wheat aphid; Triticum aestivum; Wheat ID DIURAPHIS-NOXIA HOMOPTERA; VIRUS-INFECTED TOBACCO; RUSSIAN WHEAT; GENE-EXPRESSION; PLANT DEFENSE; CALLOSOBRUCHUS-MACULATUS; TRITICUM-AESTIVUM; METHYL JASMONATE; SALICYLIC-ACID; SOUTH-AFRICA AB Damage by the Russian wheat aphid (RWA), Diuraphis noxia, significantly reduces wheat and barley yields worldwide. In compatible interactions, virulent RWA populations flourish and susceptible plants suffer extensive leaf chlorophyll loss. In incompatible interactions, RWA reproduction and population growth are significantly reduced and RWA-related chlorophyll loss in resistant plants is minor. The objectives of this study were to develop an understanding of the molecular and phytochemical bases of RWA resistance in plants containing the Dnx resistance gene. Microarray, real-time polymerase chain reaction, and phytohormone assays were conducted to identify transcriptome components unique to RWA-infested Dnx plants and susceptible (Dn0) plants, and to identify and characterize putative genes involved in Dnx plant defense responses. We found that RWA-infested Dnx plants upregulated > 180 genes related to reactive oxygen species, signaling, pathogen defense, and arthropod allelochemical and physical defense. The expression of several of these genes in RWA-infested Dnx plants increased significantly from 6- to 24-h post infestation (hpi), but their expression in Dn0 plants, when present, was delayed until 48- to 96 hpi. Concentrations of 16- and 18-carbon fatty acids, trans-methyl-12-oxophytodienoic acid, and abscisic acid were significantly greater in Dnx foliage than in Dn0 foliage after RWA infestation, suggesting that Dnx RWA defense and resistance genes may be regulated via the oxylipin pathway. These findings provide a foundation for the elucidation of the molecular basis for compatible- and incompatible plant-aphid interactions. C1 [Smith, Charles Michael; Starkey, Sharon] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Wang, Liang J.] Clemson Univ, Div Biol, Clemson, SC 29631 USA. N Carolina State Univ, Dept Plant Biol, Raleigh, NC 27695 USA. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Plant Sci Lab, Manhattan, KS 66506 USA. [Bai, Jianfa] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. RP Smith, CM (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM cmsmith@ksu.edu FU Kansas Crop Improvement Association [08-151-J]; National Science Foundation [DBI 0421427, EPS 0236913, MCB 0455318, DBI 0521587]; Kansas Technology Enterprise Corporation; K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health [P20RR16475]; Kansas State University FX We thank Nanyan Lu for helpful comments and support in the Affymetrix array data acquisition. This research was supported by a Kansas Crop Improvement Association grant to CMS and is contribution No. 08-151-J of the Kansas Agricultural Experiment Station. This research was performed in the Gene Expression Facility at Kansas State University, which is supported through the National Science Foundation grant, DBI 0421427. Plant phytohormone analyses were performed at the Kansas Lipidomics Research Center Analytical Laboratory, supported by the Functional Genomics Consortium initiative of Kansas State University's Targeted Excellence Program. The Kansas Lipidomics Research Center is supported by National Science Foundation (EPS 0236913, MCB 0455318, DBI 0521587), Kansas Technology Enterprise Corporation, K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20RR16475), and Kansas State University. NR 94 TC 32 Z9 38 U1 4 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD MAR PY 2010 VL 36 IS 3 BP 260 EP 276 DI 10.1007/s10886-010-9756-8 PG 17 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 568RI UT WOS:000275539800003 PM 20229216 ER PT J AU Yoshinaga, N Alborn, HT Nakanishi, T Suckling, DM Nishida, R Tumlinson, JH Mori, N AF Yoshinaga, Naoko Alborn, Hans T. Nakanishi, Tomoaki Suckling, David M. Nishida, Ritsuo Tumlinson, James H. Mori, Naoki TI Fatty Acid-amino Acid Conjugates Diversification in Lepidopteran Caterpillars SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Insect-produced elicitors; Lepidopteran phylogeny; Volicitin; Insect-plant interactions ID VOLICITIN-RELATED COMPOUNDS; PLANT VOLATILES; EFFICIENT INCORPORATION; INSECT HERBIVORE; ELICITOR; LARVAE; LITURA; REGURGITANT; MAIZE; BIOSYNTHESIS AB Fatty acid amino acid conjugates (FACs) have been found in noctuid as well as sphingid caterpillar oral secretions; in particular, volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] and its biochemical precursor, N-linolenoyl-L-glutamine, are known elicitors of induced volatile emissions in corn plants. These induced volatiles, in turn, attract natural enemies of the caterpillars. In a previous study, we showed that N-linolenoyl-L-glutamine in larval Spodoptera litura plays an important role in nitrogen assimilation which might be an explanation for caterpillars synthesizing FACs despite an increased risk of attracting natural enemies. However, the presence of FACs in lepidopteran species outside these families of agricultural interest is not well known. We conducted FAC screening of 29 lepidopteran species, and found them in 19 of these species. Thus, FACs are commonly synthesized through a broad range of lepidopteran caterpillars. Since all FAC-containing species had N-linolenoyl-L-glutamine and/or N-linoleoyl-L-glutamine in common, and the evolutionarily earliest species among them had only these two FACs, these glutamine conjugates might be the evolutionarily older FACs. Furthermore, some species had glutamic acid conjugates, and some had hydroxylated FACs. Comparing the diversity of FACs with lepidopteran phylogeny indicates that glutamic acid conjugates can be synthesized by relatively primitive species, while hydroxylation of fatty acids is limited mostly to larger and more developed macrolepidopteran species. C1 [Yoshinaga, Naoko; Nishida, Ritsuo; Mori, Naoki] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan. [Suckling, David M.] New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand. [Nakanishi, Tomoaki] Tokushima Prefectural Agr, Fruit Tree Res Inst, Forestry & Fisheries Technol Ctr, Tokushima 7734301, Japan. [Alborn, Hans T.] ARS, Ctr Med Agr & Vet Entomol, Chem Unit, USDA, Gainesville, FL 32611 USA. [Yoshinaga, Naoko; Tumlinson, James H.] Penn State Univ, Dept Entomol, Ctr Chem Ecol, University Pk, PA 16802 USA. RP Mori, N (reprint author), Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan. EM mokurin@kais.kyoto-u.ac.jp RI Tumlinson, James/G-8358-2011; Suckling, David/F-7005-2010 OI Suckling, David/0000-0001-7216-9348 FU Ministry of Education, Culture, Sports, Science and Technology of Japan [15580090, 18580053, 19580122]; Japan Society for the Promotion of Science FX We thank Drs. Fujisaki, K., Ando, T., Kunimi, Y., Ichida, M., and Shimoda, M. for providing insect materials. This study was supported partly by Grants-in-aid for Scientific Research (no. 15580090, 18580053, and 19580122) and by the 21st century COE program for Innovative Food and Environmental Studies Pioneered by Entomomimetic Sciences from the Ministry of Education, Culture, Sports, Science and Technology of Japan. N.Y. was supported by a Postdoctoral Fellowship for Research Abroad from the Japan Society for the Promotion of Science. NR 24 TC 32 Z9 32 U1 1 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD MAR PY 2010 VL 36 IS 3 BP 319 EP 325 DI 10.1007/s10886-010-9764-8 PG 7 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 568RI UT WOS:000275539800009 PM 20195891 ER PT J AU Nelson, CD Reinhardt, TA Thacker, TC Beitz, DC Lippolis, JD AF Nelson, C. D. Reinhardt, T. A. Thacker, T. C. Beitz, D. C. Lippolis, J. D. TI Modulation of the bovine innate immune response by production of 1 alpha,25-dihydroxyvitamin D-3 in bovine monocytes SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE vitamin D; bovine innate immunity; nitric oxide; RANTES ID VITAMIN-D METABOLISM; MYCOBACTERIUM-BOVIS; NITRIC-OXIDE; DAIRY-COWS; 1,25-DIHYDROXYVITAMIN D-3; PERIPHERAL-BLOOD; RECEPTOR; TUBERCULOSIS; INFECTION; CALCIUM AB In cattle, the kidney has been the only known site for production of 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3] from 25-hydroxyvitamin D-3 [25(OH)D-3] by 1 alpha-hydroxylase (1 alpha-OHase). Based on human studies, it was hypothesized that bovine monocytes could produce 1,25(OH)(2)D-3 upon activation and 1,25(OH)(2)D-3 would regulate expression of vitamin D-responsive genes in monocytes. First, the effects of 1,25(OH)(2)D-3 on bovine monocytes isolated from peripheral blood were tested. Treatment of nonstimulated monocytes with 1,25(OH)(2)D-3 increased expression of the gene for the vitamin D 24-hydroxylase (24-OHase) enzyme by 51 +/- 13 fold, but 1,25(OH)(2)D-3 induction of 24-OHase expression was blocked by lipopolysaccharide (LPS) stimulation. In addition, 1,25(OH)(2)D-3 increased the gene expression of inducible nitric oxide synthase and the chemokine RANTES (regulated upon activation, normal T-cell expressed and secreted) in LPS-stimulated monocytes 69 +/- 13 and 40 +/- 12 fold, respectively. Next, the ability of bovine monocytes to express 1 alpha-OHase and produce 1,25(OH)(2)D-3 was tested. Activation of monocytes with LPS, tripalmitoylated lipopeptide (Pam3CSK4), or peptidoglycan caused 43 +/- 9, 17 +/- 3, and 19 +/- 3 fold increases in 1 alpha-OHase gene expression, respectively. Addition of 25(OH)D-3 to LPS-stimulated monocytes enhanced expression of inducible nitric oxide synthase and RANTES and nitric oxide production in a dose-dependent manner, giving evidence that activated monocytes convert 25(OH)D-3 to 1,25(OH)(2)D-3. In conclusion, bovine monocytes produce 1,25(OH)(2)D-3 in response to toll-like receptor signaling, and 1,25(OH)(2)D-3 production in monocytes increased the expression of genes involved in the innate immune system. Vitamin D status of cattle might be important for optimal innate immune function because 1,25(OH)(2)D-3 production in activated monocytes and subsequent upregulation of inducible nitric oxide synthase and RANTES expression was dependent on 25(OH)D-3 availability. C1 [Nelson, C. D.; Reinhardt, T. A.; Lippolis, J. D.] ARS, Periparturient Dis Cattle Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Nelson, C. D.; Beitz, D. C.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Thacker, T. C.] ARS, Bacterial Dis Cattle Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Beitz, D. C.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Lippolis, JD (reprint author), ARS, Periparturient Dis Cattle Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM john.lippolis@ars.usda.gov RI Reinhardt, Timothy/A-7536-2009; OI Reinhardt, Timothy/0000-0001-5552-2509; Nelson, Corwin/0000-0003-0195-5610 NR 33 TC 25 Z9 26 U1 0 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2010 VL 93 IS 3 BP 1041 EP 1049 DI 10.3168/jds.2009-2663 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 562MQ UT WOS:000275056100022 PM 20172224 ER PT J AU Calvo, MS Gerry, AC McGarvey, JA Armitage, TL Mitloehner, FM AF Calvo, M. S. Gerry, A. C. McGarvey, J. A. Armitage, T. L. Mitloehner, F. M. TI Acidification of calf bedding reduces fly development and bacterial abundance SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE calf; fly control; acidification; bacteria ID LITTER TREATMENT(R) PLT(R); ESCHERICHIA-COLI O157-H7; STABLE FLIES DIPTERA; MUSCA-DOMESTICA; IMMATURE HOUSE; POULTRY LITTER; SODIUM BISULFATE; DAIRY SLURRY; EGG-YOLK; MUSCIDAE AB Environmental stressors, such as high fly density, can affect calf well-being. Sodium bisulfate (SBS) is an acidifier that reduces the pH of flooring and bedding, creating a medium that neither bacteria nor immature flies (also known as larvae or maggots) can thrive in. Two experiments were conducted to investigate the application of SBS to a mixture of rice hull calf bedding and calf slurry (BED) to reduce house fly (Musca domestica L.) larval density and the abundance of bacteria. In experiment 1, dish pans containing 1 L of BED and 3,000 house fly eggs were treated with SBS at concentrations of 0, 8.9, 17.7, and 26.5 g of SBS/0.05 m(2) of BED (CON, LOW, MED, and HIGH, respectively), with each SBS concentration applied to 4 individual pans (16 pans total). Reapplication of the same SBS concentrations in each pan occurred 3 times/wk throughout the 23-d trial. Larval house fly survival was significantly reduced in all pans with SBS relative to CON pans, with lowest survival rates in the MED and HIGH pans (99% and 100% reduction, respectively). The mean pH for each treatment was inversely related to the SBS concentration. In experiment 2, pans containing 1 L of BED and 3,000 house fly eggs were treated with either 0 g of SBS (CON), 8.9 g of SBS/0.05 m2 of BED with reapplication of the acidifier 3 times/wk (SB3x), or 8.9 g of SBS/0.05 m2 of BED applied only once at 48 h before the end of the 8 d-trial (SB48). Larval house fly survival and bacterial concentrations were reduced (90% larval reduction and 68% bacterial reduction) in the SB3x treatment relative to the CON. Mean pH was also reduced in SB3x pans relative to CON or SB48 pans. Overall, acidification of calf BED using the acidifier SBS resulted in a reduction of bacteria and house fly larval survival. This form of fly control might be expected to reduce adult fly production and, therefore, fly-related stress in calves. C1 [Calvo, M. S.; Armitage, T. L.; Mitloehner, F. M.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Gerry, A. C.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [McGarvey, J. A.] USDA ARS, Plant Mycotoxin Res Unit, Albany, CA 94710 USA. RP Mitloehner, FM (reprint author), Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. EM fmmitloehner@ucdavis.edu NR 37 TC 9 Z9 9 U1 0 U2 5 PU AMER DAIRY SCIENCE ASSOC-ADSA PI CHAMPAIGN PA 2441 VILLAGE GREEN PL, CHAMPAIGN, IL 61822 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2010 VL 93 IS 3 BP 1059 EP 1064 DI 10.3168/jds.2009-2797 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 562MQ UT WOS:000275056100024 PM 20172226 ER PT J AU Krizsan, SJ Ahvenjarvi, S Volden, H Broderick, GA AF Krizsan, S. J. Ahvenjarvi, S. Volden, H. Broderick, G. A. TI Estimation of rumen outflow in dairy cows fed grass silage-based diets by use of reticular sampling as an alternative to sampling from the omasal canal SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy cow; omasal canal; reticulum; ruminal flow ID MICROBIAL PROTEIN-SYNTHESIS; DUODENAL DIGESTA; FLOW; MARKERS; CATTLE; FIBER AB A study was conducted to compare nutrient flows determined by a reticular sampling technique with those made by sampling digesta from the omasal canal. Six lactating dairy cows fitted with ruminal cannulas were used in a design with a 3 x 2 factorial arrangement of treatments and 4 periods. Treatments were 3 grass silages differing mainly in neutral detergent fiber (NDF) concentrations: 412, 530, or 639 g/kg of dry matter, each combined with 1 of 2 levels of concentrate feed. Digesta was collected from the reticulum and the omasal canal to represent a 24-h feeding cycle. Nutrient flow was calculated using the reconstitution system based on 3 markers (Co, Yb, and indigestible NDF) and using (15)N as a microbial marker. Large and small particles and the fluid phase were recovered from digesta collected at both sampling sites. Bacterial samples from the reticulum and the omasum were separated into liquid-and particle-associated bacteria. Reticular samples were sieved through a 1-mm sieve before isolation of digesta phases and bacteria. Composition of the large particle phase differed mainly in fiber content of the digesta obtained from the 2 sampling sites. Sampling site did not affect marker concentration in any of the phases with which the markers were primarily associated. The 15N enrichment of bacterial samples did not differ between sampling sites. The reticular and omasal canal sampling techniques gave similar estimates of marker concentrations in reconstituted digesta, estimates of ruminal flow, and ruminal digestibility values for dry matter, organic matter, starch, and N. Sampling site x diet interactions were also not significant. Concentration of NDF was 2.2% higher in reconstituted omasal digesta than in reconstituted reticular digesta. Ruminal NDF digestibility was 2.7% higher when estimated by sampling the reticulum than by sampling the omasal canal. The higher estimate of ruminal NDF digestibility with the reticular sampling technique was due to differences in NDF concentration of reconstituted digesta. This study shows that nutrient and microbial protein outflow from the rumen can be measured using a reticular sampling technique. The reticular sampling technique provides a promising alternative to sampling from the omasal canal because there is less interference with the animal and it does not require advanced sampling equipment. C1 [Krizsan, S. J.; Volden, H.] Norwegian Univ Life Sci, Dept Anim & Aquacultural Sci, As, Norway. [Ahvenjarvi, S.] MTT Agrifood Res Finland, Anim Prod Res, Jokioinen, Finland. [Broderick, G. A.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Krizsan, SJ (reprint author), Norwegian Univ Life Sci, Dept Anim & Aquacultural Sci, As, Norway. EM sophie.krizsan@umb.no RI Ahvenjarvi, Seppo/C-5527-2009 NR 25 TC 11 Z9 11 U1 1 U2 13 PU AMER DAIRY SCIENCE ASSOC-ADSA PI CHAMPAIGN PA 2441 VILLAGE GREEN PL, CHAMPAIGN, IL 61822 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2010 VL 93 IS 3 BP 1138 EP 1147 DI 10.3168/jds.2009-2661 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 562MQ UT WOS:000275056100033 PM 20172235 ER PT J AU Coblentz, WK Hoffman, PC Martin, NP AF Coblentz, W. K. Hoffman, P. C. Martin, N. P. TI Effects of spontaneous heating on forage protein fractions and in situ disappearance kinetics of crude protein for alfalfa-orchardgrass hays packaged in large round bales SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE acid detergent insoluble crude protein; crude protein disappearance kinetics; neutral detergent insoluble crude protein; spontaneous heating ID DETERGENT-INSOLUBLE NITROGEN; BERMUDAGRASS HAY; NUTRITIVE-VALUE; DRY-MATTER; MOISTURE-CONTENT; EASTERN GAMAGRASS; FIBER DEGRADATION; STORAGE; INVITRO; STANDARDIZATION AB During 2006 and 2007, forages from 3 individual hay harvests were used to assess the effects of spontaneous heating on concentrations of crude protein (CP), neutral detergent insoluble CP (NDICP), acid detergent insoluble CP (ADICP), and in situ disappearance kinetics of CP and NDICP for large round bales of mixed alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.). Over the 3 harvests, 96 large round bales were made at preset bale diameters of 0.9, 1.2, or 1.5 m and at moisture concentrations ranging from 9.3 to 46.6%. Internal bale temperatures were monitored daily during an outdoor storage period. The change in concentrations of NDICP (poststorage-prestorage) increased with heating degree days (HDD) > 30 degrees C in a relationship best explained with a nonlinear model {Y = 24.9 - [22.7 x (e(-0.000010 x x x x))]; R(2) = 0.892} that became asymptotic at + 24.9 percentage units of CP, thereby indicating that NDICP increases rapidly within bales that heat spontaneously. When maximum internal bale temperature (MAX) was used as the independent variable, the best regression model was quadratic and the coefficient of determination was still relatively high (R(2) = 0.716). The change in concentrations of ADICP (poststorage - prestorage; Delta ADICP) also increased with HDD and was best fitted to a nonlinear model {Y = 14.9 - [15.7 x (e-(0.0000019 x x x x))]} with a very high coefficient of determination (R(2) = 0.934). A similar quartic response was observed for the regression of Delta ADICP on MAX (R(2) = 0.975). Increases in Delta ADICP as a result of heating (HDD or MAX) were paralleled by concurrent increases in hemicellulose at relatively low increments of heating, but the inverse relationship was observed as hemicelluloses likely became reactive and concentrations decreased in more severely heated hays. Changes in ruminal disappearance rate of CP were best fitted to cubic models for regressions on both HDD (R(2) = 0.939) and MAX (R(2) = 0.876); these changes represented an approximate 50% rate reduction in severely heated hays relative to prestorage controls. Within ranges of heating most commonly encountered under field conditions, changes in rumen-degradable protein decreased in a primarily linear relationship with HDD or MAX. However, the mean change in rumen-degradable protein for the 4 most severely heated hays was only - 2.6 percentage units of CP, which represents a minimal reduction from prestorage controls and is far less than the maximum of -7.9 percentage units of CP observed with less-severe heating. Interpretation of these results was complicated by poor recovery of NDICP from our most severely heated hays following machine rinsing of 0-h Dacron bags; theoretically, and by definition, this unrecovered pool of NDICP is assumed to be entirely degradable in the rumen. It remains unclear whether these responses could be corroborated in vivo or by other analytical techniques, or whether the magnitude of HDD or MAX for our most severely heated hays exceeds the reliable limits for estimating RDP via in situ methodology. C1 [Coblentz, W. K.] ARS, USDA, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. [Hoffman, P. C.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Martin, N. P.] US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Coblentz, WK (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. EM wayne.coblentz@ars.usda.gov NR 36 TC 11 Z9 11 U1 2 U2 4 PU AMER DAIRY SCIENCE ASSOC-ADSA PI CHAMPAIGN PA 2441 VILLAGE GREEN PL, CHAMPAIGN, IL 61822 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2010 VL 93 IS 3 BP 1148 EP 1169 DI 10.3168/jds.2009-2701 PG 22 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 562MQ UT WOS:000275056100034 PM 20172236 ER PT J AU Rotz, CA Montes, F Chianese, DS AF Rotz, C. A. Montes, F. Chianese, D. S. TI The carbon footprint of dairy production systems through partial life cycle assessment SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE greenhouse gas; carbon footprint; farm model; DairyGHG ID GREENHOUSE-GAS EMISSIONS; NITROUS-OXIDE EMISSIONS; REDUCTION STRATEGIES; METHANE EMISSIONS; DANISH AGRICULTURE; GRAZING ANIMALS; MILK-PRODUCTION; UNITED-STATES; ENERGY; FARMS AB Greenhouse gas (GHG) emissions and their potential effect on the environment has become an important national and international issue. Dairy production, along with all other types of animal agriculture, is a recognized source of GHG emissions, but little information exists on the net emissions from dairy farms. Component models for predicting all important sources and sinks of CH(4), N(2)O, and CO(2) from primary and secondary sources in dairy production were integrated in a software tool called the Dairy Greenhouse Gas model, or DairyGHG. This tool calculates the carbon footprint of a dairy production system as the net exchange of all GHG in CO(2) equivalent units per unit of energy-corrected milk produced. Primary emission sources include enteric fermentation, manure, cropland used in feed production, and the combustion of fuel in machinery used to produce feed and handle manure. Secondary emissions are those occurring during the production of resources used on the farm, which can include fuel, electricity, machinery, fertilizer, pesticides, plastic, and purchased replacement animals. A longterm C balance is assumed for the production system, which does not account for potential depletion or sequestration of soil carbon. An evaluation of dairy farms of various sizes and production strategies gave carbon footprints of 0.37 to 0.69 kg of CO(2) equivalent units/kg of energy-corrected milk, depending upon milk production level and the feeding and manure handling strategies used. In a comparison with previous studies, DairyGHG predicted C footprints similar to those reported when similar assumptions were made for feeding strategy, milk production, allocation method between milk and animal coproducts, and sources of CO(2) and secondary emissions. DairyGHG provides a relatively simple tool for evaluating management effects on net GHG emissions and the overall carbon footprint of dairy production systems. C1 [Rotz, C. A.; Montes, F.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Chianese, D. S.] ENVIRON Int Corp, Los Angeles, CA 90017 USA. RP Rotz, CA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM al.rotz@ars.usda.gov NR 63 TC 96 Z9 106 U1 14 U2 87 PU AMER DAIRY SCIENCE ASSOC-ADSA PI CHAMPAIGN PA 2441 VILLAGE GREEN PL, CHAMPAIGN, IL 61822 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2010 VL 93 IS 3 BP 1266 EP 1282 DI 10.3168/jds.2009-2162 PG 17 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 562MQ UT WOS:000275056100045 PM 20172247 ER PT J AU Travers, SE Tang, ZW Caragea, D Garrett, KA Hulbert, SH Leach, JE Bai, JF Saleh, A Knapp, AK Fay, PA Nippert, J Schnable, PS Smith, MD AF Travers, Steven E. Tang, Zhongwen Caragea, Doina Garrett, Karen A. Hulbert, Scott H. Leach, Jan E. Bai, Jianfa Saleh, Amgad Knapp, Alan K. Fay, Philip A. Nippert, Jesse Schnable, Patrick S. Smith, Melinda D. TI Variation in gene expression of Andropogon gerardii in response to altered environmental conditions associated with climate change SO JOURNAL OF ECOLOGY LA English DT Article DE ecological genomics; enrichment analysis; gene function; Gene Ontology; microarray; precipitation variability; Rainfall Manipulation Plots; tallgrass prairie; transcription profile; warming ID WATER-USE EFFICIENCY; MESIC GRASSLAND; POPULATIONS; STRESS; CHLOROPHYLL; ECOGENOMICS; ADAPTATION; CHALLENGES; ECOSYSTEM; PROFILES AB 1. If we are to understand the mechanisms underlying species responses to climate change in natural systems, studies are needed that focus on responses of non-model species under field conditions. We measured transcriptional profiles of individuals of Andropogon gerardii, a C(4) grass native to North American grasslands, in a field experiment in which both temperature and precipitation were manipulated to simulate key aspects of forecasted climate change. 2. By using microarrays developed for a closely related model species, Zea mays, we were able to compare the relative influence of warming versus altered soil moisture availability on expression levels of over 7000 genes, identify responsive functional groups of genes and correlate changes in gene transcription with physiological responses. 3. We observed more statistically significant shifts in transcription levels of genes in response to thermal stress than in response to water stress. We also identified candidate genes that demonstrated transcription levels closely associated with physiological variables, in particular chlorophyll fluorescence. 4. Synthesis. These results suggest that an ecologically important species responds differently to different environmental aspects of forecast climate change. These translational changes have the potential to influence phenotypic characters and ultimately adaptive responses. C1 [Travers, Steven E.; Tang, Zhongwen; Garrett, Karen A.; Bai, Jianfa; Saleh, Amgad] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Caragea, Doina] Kansas State Univ, Dept Comp Sci, Manhattan, KS 66506 USA. [Hulbert, Scott H.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Knapp, Alan K.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Nippert, Jesse] Kansas State Univ, Dept Biol, Manhattan, KS 66506 USA. [Schnable, Patrick S.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Smith, Melinda D.] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT 06520 USA. RP Travers, SE (reprint author), Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. EM steven.travers@ndsu.edu RI Saleh, Amgad/A-8697-2012; Smith, Melinda/J-8987-2014; Young, Kristina/M-3069-2014; OI Saleh, Amgad/0000-0002-2354-0144; Fay, Philip/0000-0002-8291-6316 FU NSF [DEB-0130692, DBI-0421427]; USDA CSREES Ecosystems Studies Program; Office of Science (PER), US Department of Energy [DE-FG02-04ER63892]; Kansas Agricultural Experiment Station [09-218-J] FX Research assistance was provided by Shauna Dendy, Kathryn Wagner and Casey Anderson. George Milliken provided guidance in the statistical analysis of our results. The Kansas EPSCoR Ecological Genomics consortium provided financial support and lively discussion of this work. Our study was funded by NSF Grant #DEB-0130692, the NSF Long Term Ecological Research Program at Konza Prairie, the USDA CSREES Ecosystems Studies Program, the Office of Science (PER), US Department of Energy Grant #DE-FG02-04ER63892, NSF Grant #DBI-0421427 and the Kansas Agricultural Experiment Station (Contribution #09-218-J). Microarray data files are available at GEO-NCBI data bank and can be accessed through the series ID number GSE16418 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16418). NR 37 TC 22 Z9 22 U1 1 U2 25 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-0477 J9 J ECOL JI J. Ecol. PD MAR PY 2010 VL 98 IS 2 BP 374 EP 383 DI 10.1111/j.1365-2745.2009.01618.x PG 10 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA 548HF UT WOS:000273950400013 ER PT J AU Venuto, M Halbrook, B Hinners, M Lange, A Mickelson, S AF Venuto, Margaret Halbrook, Brenda Hinners, Marion Lange, Audrina Mickelson, Stephanie TI Analyses of the eFORS (Electronic Foodborne Outbreak Reporting System) Surveillance Data (2000-2004) in School Settings SO JOURNAL OF ENVIRONMENTAL HEALTH LA English DT Article ID UNITED-STATES; DISEASE OUTBREAKS; NOROVIRUS; ENGLAND AB Frequently cited statistics indicate that the burden of foodborne disease is a serious public health problem, particularly for vulnerable populations such as children. The purpose of the descriptive retrospective study discussed in this article was to analyze data collected within the Electronic Foodborne Outbreak Reporting System (eFORS) in school settings in order to examine the magnitude of foodborne disease etiologies and to recommend strategies for prevention. Data on foodborne outbreaks (N = 96) and illnesses (N = 6,367) were extracted and analyzed from the Centers for Disease Control and Prevention's (CDC's) eFORS spanning the years 2000 to 2004. C1 [Venuto, Margaret] Food & Nutr Serv, USDA, Alexandria, VA 22302 USA. RP Venuto, M (reprint author), Food & Nutr Serv, USDA, 3101 Pk Ctr Dr,Suite 512, Alexandria, VA 22302 USA. EM margaretve-nuto@yahoo.com NR 19 TC 2 Z9 2 U1 0 U2 1 PU NATL ENVIRON HEALTH ASSOC PI DENVER PA 720 S COLORADO BLVD SUITE 970, SOUTH TOWER, DENVER, CO 80246 USA SN 0022-0892 J9 J ENVIRON HEALTH JI J. Environ. Health PD MAR PY 2010 VL 72 IS 7 BP 8 EP 13 PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA 563TE UT WOS:000275156300002 PM 20235403 ER PT J AU Heller, H Bar-Tal, A Tamir, G Bloom, P Venterea, RT Chen, D Zhang, Y Clapp, CE Fine, P AF Heller, Hadar Bar-Tal, Asher Tamir, Guy Bloom, Paul Venterea, Rodney T. Chen, Dong Zhang, Yi Clapp, C. Edward Fine, Pinchas TI Effects of Manure and Cultivation on Carbon Dioxide and Nitrous Oxide Emissions from a Corn Field under Mediterranean Conditions SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID GREENHOUSE-GAS EMISSIONS; SOIL CARBON; AVAILABLE NITROGEN; CROPPING SYSTEMS; N2O EMISSIONS; TILLAGE; FLUXES; CO2; SEQUESTRATION; MANAGEMENT AB The use of organic residues as soil additives is increasing, but, depending on their composition and application methods, these organic amendments can stimulate the emissions of CO(2) and N(2)O. The objective of this Study was to quantify the effects of management practices in irrigated sweet corn (Zea mays L.) on CO(2) and N(2)O emissions and to relate emissions to environmental factors. In a 3-yr study, corn residues (CR) and pasteurized chicken manure (PCM) Were used as soil amendments compared with no residue (NR) under three management practices: shallow tillage (ST) and no tillage (NT) under consecutive corn crops and ST Without crop. Tillage significantly increased (P < 0.05) CO(2) and N(2)O fluxes in residue-amended plots and in NR plots. Carbon dioxide and N(2)O fluxes were correlated with soil NH(4) concentrations and with days since tillage and days since seeding, Fluxes of CO(2) were correlated with soil water content, whereas N(2)O flux had higher correlation with air temperature. Annual CO(2) emissions were higher with PCM than with CR and NR (9.7, 2.9, and 2.3 Mg C ha(-1), respectively). Fluxes of N(2)O were 34.4, 0.94, and 0.77 kg N ha(-1) yr(-1) with PCM, CR, and NR, respectively. Annual amounts of CO(2)-C and N(2)O-N emissions from the PCM treatments were 64 and 3% of the applied C and N, respectively. Regardless of cultivation practices, elevated N(2)O emissions were recorded in the PCM treatment. These emissions could negate some of the beneficial effects of PCM on soil properties. C1 [Heller, Hadar; Bar-Tal, Asher; Tamir, Guy; Fine, Pinchas] Agr Res Org, Volcani Ctr, Inst Soil Water & Environm Sci, IL-50250 Bet Dagan, Israel. [Bloom, Paul; Venterea, Rodney T.; Clapp, C. Edward] Univ Minnesota, Dep Soil Water & Climate, St Paul, MN 55108 USA. [Venterea, Rodney T.; Clapp, C. Edward] Univ Minnesota, USDA ARS, St Paul, MN 55108 USA. [Chen, Dong] Calif Dept Water Resources, Sacramento, CA 94236 USA. [Zhang, Yi] Manitoba Agr Initiat, Agri Environm Branch, Winnipeg, MB R0J 1E0, Canada. [Zhang, Yi] Manitoba Food Initiat, Agri Environm Branch, Winnipeg, MB R0J 1E0, Canada. [Zhang, Yi] Manitoba Rural Initiat, Agri Environm Branch, Winnipeg, MB R0J 1E0, Canada. RP Heller, H (reprint author), Agr Res Org, Volcani Ctr, Inst Soil Water & Environm Sci, POB 6, IL-50250 Bet Dagan, Israel. EM hadarh@agri.gov.il RI Bar-Tal, Asher/A-8144-2012; Venterea, Rodney/A-3930-2009 FU BARD [IS-3517-04]; United States-Israel Binational Agricultural Research and Development Fund FX This research was supported by Research Grant Award No. IS-3517-04 from BARD, The United States-Israel Binational Agricultural Research and Development Fund. We thank Mr. Tibor Markowitz for his significant contribution in setting the field experiment and performing soil moisture measurements. NR 41 TC 30 Z9 30 U1 4 U2 24 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2010 VL 39 IS 2 BP 437 EP 448 DI 10.2134/jeq2009.0027 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA 566PA UT WOS:000275383600001 PM 20176817 ER PT J AU Kukier, U Chaney, RL Ryan, JA Daniels, WL Dowdy, RH Granato, TC AF Kukier, Urszula Chaney, Rufus L. Ryan, James A. Daniels, W. Lee Dowdy, Robert H. Granato, Thomas C. TI Phytoavailability of Cadmium in Long-Term Biosolids-Amended Soils SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ISOTOPIC DILUTION TECHNIQUES; SEWAGE-SLUDGE; ZINC AVAILABILITY; ORGANIC-CARBON; HEAVY-METALS; ACCUMULATION; CORN; PHOSPHORUS; BIOAVAILABILITY; PENNSYLVANIA AB Agronomic use of biosolids has raised concern that plant availability of biosolids-Cd will increase with time after cessation of biosolids application. It has been demonstrated that chemical extractability of Cd is persistently decreased in biosolids-amended soils. This study was conducted to determine if Cd phytoavailability in long-term biosolids-amended soils was also persistently decreased. Paired control and biosolids-amended soils were collected from three experimental sites where large cumulative rates of biosolids were applied about 20 yr ago. The pH of all soils [in 0.01 mol L(-1)Ca(NO(3))(2)] was adjusted to 6.5 +/- 0.2. Increasing rates of Cd-nitrate (from 0 to 10.0 mg Cd kg(-1) soil) enriched in (111)Cd stable isotope were added to all soils, and Romaine lettuce (Lactuca sativa L. var. longifolia Lam.) was grown in pots to bioassay phytoavailable Cd. After harvest, Cd concentrations in shoots and labile pool of Cd (Cd(L)) in soils were determined. The relationship between added salt-Cd and Cd concentrations in lettuce shoots was linear for all soils tested. Ratios of (shoot Cd):(soil Cd) slopes were highest in the control soils. Biosolids amendment decreased (shoot Cd):(soil Cd) slopes to varied extent depending on biosolids source, properties, and application rate. The decrease in slope in comparison to the control was an indication of the lower phytoavailability of Cd in biosolids-amended soils. A significant negative correlation existed between Cd uptake slopes and soil organic matter, free and amorphous Fe and Al oxides, Bray-P and soil and plant Zoo. Biosolids-Cd was highly labile (%L 80-95) except for Fulton County soil (%L = 61). C1 [Chaney, Rufus L.] USDA ARS, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [Kukier, Urszula; Daniels, W. Lee] Virginia Polytech Inst & State Univ, Dep Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Ryan, James A.] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45224 USA. [Dowdy, Robert H.] Univ Minnesota, USDA ARS, St Paul, MN 55108 USA. [Granato, Thomas C.] Metropolitan Water Reclamat Dist Greater Chicago, Cicero, IL 60804 USA. RP Chaney, RL (reprint author), USDA ARS, Environm Management & Byprod Utilizat Lab, 10300 Baltimore Blvd, Beltsville, MD 20705 USA. EM Rufus.Chaney@ars.usda.gov FU US-EPA; USDA-ARS FX The authors gratefully acknowledge Dr. C. Veillon and Dr. K.Y. Patterson for their helpful advice and assistance with Cd isotope measurements. We also thank Dr. C. Green for maintaining analytical equipment used for metal analysis and J.E. Woolston for technical assistance. Appreciation is expressed to C. Carlson, J. DeWees, E. Boucek, and R. Swango for collecting Fulton County soils and M. Dolan for collecting the Minnesota soils. This project was supported by an Interagency Agreement between US-EPA and USDA-ARS. NR 68 TC 25 Z9 25 U1 4 U2 41 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2010 VL 39 IS 2 BP 519 EP 530 DI 10.2134/jeq2007.0671 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA 566PA UT WOS:000275383600009 PM 20176825 ER PT J AU Prior, SA Runion, GB Rogers, HH Arriaga, FJ AF Prior, S. A. Runion, G. B. Rogers, H. H. Arriaga, F. J. TI Elevated Atmospheric Carbon Dioxide Effects on Soybean and Sorghum Gas Exchange in Conventional and No-Tillage Systems SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID TEXAS BLACKLAND PRAIRIE; CROP PRODUCTION; CONSERVATION TILLAGE; PLANT-RESPONSES; CO2 ENRICHMENT; WHEAT; WATER; ROTATION; SOIL; MANAGEMENT AB Increasing atmospheric CO(2) concentration has led to concerns about potential effects on production agriculture. In the fall of 1997, a study was initiated to compare the response of two crop management systems (conventional tillage and no-tillage) to elevated CO(2). The study used a split-plot design replicated three times with two management systems as main plots and two atmospheric CO(2) levels (ambient and twice ambient) as split plots using open-top chambers on a Decatur silt learn soil (clayey, kaolinitic, thermic Rhodic Paleudults). The conventional system was a grain sorghum [Sorghum bicolor (L.) Moench.] and soybean (Glycine max (L.) Merr.] rotation with winter fallow and spring tillage practices. In the no-tillage system, sorghum and soybean were rotated, and three cover crops were used [crimson clover (Trifolium incarnatum L.), sunn hemp (Crotalaria juncea L.), and wheat (Triticum aestivum L.)]. Over multiple growing seasons, the effect of management and CO(2) concentration on leaf-level gas exchange during row crop (soybean in 1999, 2001, and 2003; sorghum in 2000, 2002, and 2004) reproductive growth were evaluated. Treatment effects were fairly consistent across years. In general, higher photosynthetic races were observed under CO(2) enrichment (more so with soybean) regardless of residue management practice. Elevated CO(2) led to decreases in stomatal conductance and transpiration, which resulted in increased water use efficiency. The effects of management system on gas exchange measurements were infrequently significant, as were interactions of CO(2) and management. These results Suggest that better soil moisture conservation and high rates of photosynthesis can occur in both tillage systems in CO(2)-enriched environments during reproductive growth. C1 [Prior, S. A.; Runion, G. B.; Rogers, H. H.; Arriaga, F. J.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. RP Prior, SA (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM steve.prior@ars.usda.gov FU Biological and Environmental Research Program (BER; U.S. Department of Energy [DE-AI02-95ER62088] FX The authors thank B.G. Dorman and J.W Carrington for technical assistance. This research was Supported by the Biological and Environmental Research Program (BER), U.S. Department of Energy, Interagency Agreement No. DE-AI02-95ER62088. NR 45 TC 6 Z9 8 U1 1 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2010 VL 39 IS 2 BP 596 EP 608 DI 10.2134/jeq2009.0181 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA 566PA UT WOS:000275383600017 PM 20176833 ER PT J AU Lucero, ME Estell, RE Fredrickson, E AF Lucero, Mary E. Estell, Rick E. Fredrickson, Ed TI Composition of Ceanothus gregii Oil as Determined by Steam Distillation and Solid-Phase Microextraction SO JOURNAL OF ESSENTIAL OIL RESEARCH LA English DT Article DE Ceanothus gregii; Rhamnaceae; essential oil composition; methyl salicylate; hexanal AB Ceanothus gregii Gray was collected from the Jornada Experimental Range in south central New Mexico. Current year's growth was collected from ten plants found within an approximate 50 in radius of N32 degrees 40.605' and W106 degrees 33.486' at an altitude of 1,741 in during July 2001. Composite samples of the plants were steam distilled in triplicate, and the composite oil was analyzed using both GC-FID and GC/MS. The volatile composition of the same plants was also examined using solid-phase microextraction (SPME) with a 100 mu m polydimethylsiloxane fiber. Mass spectra and retention indices were used to identify 41 previously described compounds. Methyl salicylate (16.8%), hexanal (11.8%) and decanal (7.0%) were the major identified compounds. C1 [Lucero, Mary E.; Estell, Rick E.; Fredrickson, Ed] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. RP Lucero, ME (reprint author), USDA ARS Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. NR 10 TC 0 Z9 0 U1 0 U2 2 PU ALLURED PUBL CORP PI CAROL STREAM PA 362 S SCHMALE RD, CAROL STREAM, IL 60188-2787 USA SN 1041-2905 J9 J ESSENT OIL RES JI J. Essent. Oil Res. PD MAR-APR PY 2010 VL 22 IS 2 BP 140 EP 142 PG 3 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 564KN UT WOS:000275213100015 ER PT J AU Gonda, I Bar, E Portnoy, V Lev, S Burger, J Schaffer, AA Tadmor, Y Gepstein, S Giovannoni, JJ Katzir, N Lewinsohn, E AF Gonda, Itay Bar, Einat Portnoy, Vitaly Lev, Shery Burger, Joseph Schaffer, Arthur A. Tadmor, Ya'akov Gepstein, Shimon Giovannoni, James J. Katzir, Nurit Lewinsohn, Efraim TI Branched-chain and aromatic amino acid catabolism into aroma volatiles in Cucumis melo L. fruit SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Amino acid metabolism; amino acid transaminase; aromatic amino acids; branched-chain amino acids; Cucumis melo; melon aroma; volatile aroma compounds ID TYROSINE AMINOTRANSFERASE; SACCHAROMYCES-CEREVISIAE; FUNCTIONAL-CHARACTERIZATION; ALCOHOL ACYLTRANSFERASE; HUMAN MITOCHONDRIAL; TRYPANOSOMA-CRUZI; TOMATO AROMA; GENE FAMILY; BIOSYNTHESIS; ARABIDOPSIS AB The unique aroma of melons (Cucumis melo L., Cucurbitaceae) is composed of many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino acids, and terpenes. Although amino acids are known precursors of aroma compounds in the plant kingdom, the initial steps in the catabolism of amino acids into aroma volatiles have received little attention. Incubation of melon fruit cubes with amino acids and alpha-keto acids led to the enhanced formation of aroma compounds bearing the side chain of the exogenous amino or keto acid supplied. Moreover, L-[(13)C(6)]phenylalanine was also incorporated into aromatic volatile compounds. Amino acid transaminase activities extracted from the flesh of mature melon fruits converted L-isoleucine, L-leucine, L-valine, L-methionine, or L-phenylalanine into their respective alpha-keto acids, utilizing alpha-ketoglutarate as the amine acceptor. Two novel genes were isolated and characterized (CmArAT1 and CmBCAT1) encoding 45.6 kDa and 42.7 kDa proteins, respectively, that displayed aromatic and branched-chain amino acid transaminase activities, respectively, when expressed in Escherichia coli. The expression of CmBCAT1 and CmArAT1 was low in vegetative tissues, but increased in flesh and rind tissues during fruit ripening. In addition, ripe fruits of climacteric aromatic cultivars generally showed high expression of CmBCAT1 and CmArAT1 in contrast to non-climacteric non-aromatic fruits. The results presented here indicate that in melon fruit tissues, the catabolism of amino acids into aroma volatiles can initiate through a transamination mechanism, rather than decarboxylation or direct aldehyde synthesis, as has been demonstrated in other plants. C1 [Gonda, Itay; Bar, Einat; Portnoy, Vitaly; Lev, Shery; Burger, Joseph; Schaffer, Arthur A.; Tadmor, Ya'akov; Katzir, Nurit; Lewinsohn, Efraim] Agr Res Org, Newe Yaar Res Ctr, Inst Plant Sci, IL-30095 Ramat Yishay, Israel. [Gonda, Itay; Gepstein, Shimon] Technion Israel Inst Technol, Fac Biol, IL-32000 Haifa, Israel. [Giovannoni, James J.] Cornell Univ, USDA, Ithaca, NY 14853 USA. [Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Lewinsohn, E (reprint author), Agr Res Org, Newe Yaar Res Ctr, Inst Plant Sci, POB 1021, IL-30095 Ramat Yishay, Israel. EM twefraim@agri.gov.il FU BARD [IS-3877-06]; United States-Israel Binational Agricultural Research and Development; EU [FOOD-CT-2006-036220] FX We thank Uzi Sa'ar, Rotem Harel-Beja, and Fabian Baumkoler for growing the plants, and Uzi Ravid, Rachel Davidovich-Rikanati, and Yaron Sitrit for helpful discussions. This work was partially supported by grant No. IS-3877-06 of BARD, the United States-Israel Binational Agricultural Research and Development Fund, and by the EU within the plant metabolomics project META-PHOR (FOOD-CT-2006-036220). Publication No. 134/2009 of the Agricultural Research Organization, Bet Dagan Israel. NR 73 TC 78 Z9 86 U1 2 U2 34 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2010 VL 61 IS 4 BP 1111 EP 1123 DI 10.1093/jxb/erp390 PG 13 WC Plant Sciences SC Plant Sciences GA 569AD UT WOS:000275567300015 PM 20065117 ER PT J AU Sbabou, L Bucciarelli, B Miller, S Liu, JQ Berhada, F Filali-Maltouf, A Allan, D Vance, C AF Sbabou, Laila Bucciarelli, Bruna Miller, Susan Liu, Junqi Berhada, Fatiha Filali-Maltouf, Abdelkarim Allan, Deborah Vance, Carroll TI Molecular analysis of SCARECROW genes expressed in white lupin cluster roots SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Cluster roots; endodermis; interfering RNA; Lupinus albus; scarecrow; short roots ID ASYMMETRIC CELL-DIVISION; PROTEOID ROOTS; PHOSPHORUS DEFICIENCY; ARABIDOPSIS-THALIANA; ENHANCED EXPRESSION; PHOSPHATE STARVATION; RADIAL ORGANIZATION; ALBUS L.; PLANTS; REVEALS AB The Scarecrow (SCR) transcription factor plays a crucial role in root cell radial patterning and is required for maintenance of the quiescent centre and differentiation of the endodermis. In response to phosphorus (P) deficiency, white lupin (Lupinus albus L.) root surface area increases some 50-fold to 70-fold due to the development of cluster (proteoid) roots. Previously it was reported that SCR-like expressed sequence tags (ESTs) were expressed during early cluster root development. Here the cloning of two white lupin SCR genes, LaSCR1 and LaSCR2, is reported. The predicted amino acid sequences of both LaSCR gene products are highly similar to AtSCR and contain C-terminal conserved GRAS family domains. LaSCR1 and LaSCR2 transcript accumulation localized to the endodermis of both normal and cluster roots as shown by in situ hybridization and gene promoter::reporter staining. Transcript analysis as evaluated by quantitative real-time-PCR (qRT-PCR) and RNA gel hybridization indicated that the two LaSCR genes are expressed predominantly in roots. Expression of LaSCR genes was not directly responsive to the P status of the plant but was a function of cluster root development. Suppression of LaSCR1 in transformed roots of lupin and Medicago via RNAi (RNA interference) delivered through Agrobacterium rhizogenes resulted in decreased root numbers, reflecting the potential role of LaSCR1 in maintaining root growth in these species. The results suggest that the functional orthologues of AtSCR have been characterized. C1 [Sbabou, Laila; Liu, Junqi; Vance, Carroll] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Sbabou, Laila; Berhada, Fatiha; Filali-Maltouf, Abdelkarim] Univ Mohammed 5, Fac Sci, Lab Microbiol & Biol Mol, Rabat, Morocco. [Bucciarelli, Bruna; Miller, Susan; Vance, Carroll] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. [Allan, Deborah] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Vance, C (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM vance004@umn.edu OI FILALI-MALTOUF, Abdelkarim/0000-0002-2727-3389 FU United States Department of Agriculture [2005-35100-16002]; USDA Agricultural Research Service CRIS [3640-21000-024-00D]; Fulbright Scholar Program; Moroccan-American Commission for Educational & Cultural Exchange FX This work was supported by United States Department of Agriculture, National Research Initiative, CSREES Grant Number 2005-35100-16002; USDA Agricultural Research Service CRIS number 3640-21000-024-00D; the Fulbright Scholar Program; and the Moroccan-American Commission for Educational & Cultural Exchange for financial support for this work. NR 47 TC 13 Z9 15 U1 0 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2010 VL 61 IS 5 BP 1351 EP 1363 DI 10.1093/jxb/erp400 PG 13 WC Plant Sciences SC Plant Sciences GA 569AF UT WOS:000275567500009 PM 20167612 ER PT J AU Hung, CY Sun, YH Chen, JJ Darlington, DE Williams, AL Burkey, KO Xie, JH AF Hung, Chiu-Yueh Sun, Ying-Hsuan Chen, Jianjun Darlington, Diane E. Williams, Alfred L. Burkey, Kent O. Xie, Jiahua TI Identification of a Mg-protoporphyrin IX monomethyl ester cyclase homologue, EaZIP, differentially expressed in variegated Epipremnum aureum 'Golden Pothos' is achieved through a unique method of comparative study using tissue regenerated plants SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Golden Pothos; Mg-protoporphyrin IX monomethyl ester cyclase; tissue culture; transcript abundance; variegation formation ID WHITE LEAF SECTORS; CHLOROPHYLL BIOSYNTHESIS; CHLOROPLAST DEVELOPMENT; ARABIDOPSIS CHL27; GENE ENCODES; LOCUS; FTSH; PROTEIN; MUTANT; RNA AB Variegated plants provide a valuable tool for studying chloroplast biogenesis by allowing direct comparison between green and white/yellow sectors within the same leaf. While variegated plants are abundant in nature, the mechanism of leaf variegation remains largely unknown. Current studies are limited to a few mutants in model plant species, and are complicated by the potential for cross-contamination during dissection of leaf tissue into contrasting sectors. To overcome these obstacles, an alternative approach was explored using tissue-culture techniques to regenerate plantlets from unique sectors. Stable green and pale yellow plants were developed from a naturally variegated Epipremnum aureum 'Golden Pothos'. By comparing the gene expression between green and pale yellow plants using suppression subtractive hybridization in conjunction with homologous sequence search, nine down-regulated and 18 up-regulated genes were identified in pale yellow plants. Transcript abundance for EaZIP (Epipremnum aureum leucine zipper), a nuclear gene homologue of tobacco NTZIP and Arabidopsis CHL27, was reduced more than 4000-fold in qRT-PCR analysis. EaZIP encodes the Mg-protoporphyrin IX monomethyl ester cyclase, one of the key enzymes in the chlorophyll biosynthesis pathway. Examination of EaZIP expression in naturally variegated 'Golden Pothos' confirmed that EaZIP transcript levels were correlated with leaf chlorophyll contents, suggesting that this gene plays a major role in the loss of chlorophyll in the pale yellow sectors of E. aureum 'Golden Pothos'. This study further suggests that tissue-culture regeneration of plantlets from different coloured sectors of variegated leaves can be used to investigate the underlying mechanisms of variegation. C1 [Hung, Chiu-Yueh; Darlington, Diane E.; Williams, Alfred L.; Xie, Jiahua] N Carolina Cent Univ, Biomfg Res Inst & Technol Enterprise, Dept Pharmaceut Sci, Durham, NC 27707 USA. [Sun, Ying-Hsuan] N Carolina State Univ, Coll Nat Resources, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Chen, Jianjun] Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Chen, Jianjun] Univ Florida, Dept Environm Hort, Apopka, FL 32703 USA. [Burkey, Kent O.] N Carolina State Univ, ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Burkey, Kent O.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Xie, JH (reprint author), N Carolina Cent Univ, Biomfg Res Inst & Technol Enterprise, Dept Pharmaceut Sci, Durham, NC 27707 USA. EM jxie@nccu.edu OI Xie, Jiahua/0000-0003-2786-0281 FU National Institute of General Medical Sciences [SC3GM088084] FX The work was mainly carried out: North Carolina Central University and North Carolina State University. This work was supported by Award Number SC3GM088084 from the National Institute of General Medical Sciences to JX and a Startup Fund of Golden LEAF Foundation to BRITE. We thank Lisa Lentz and Dr Wayne P Robarge of the Analytical Laboratory Service at North Carolina State University, and Dr Brenda R Cleveland of the Agronomic Division Plant/Waste/Solution Laboratory at North Carolina Department of Agriculture and Consumer Services for analysing element contents. Special thanks to Valerie M Knowlton of the Center for Electron Microscopy at the North Carolina State University for help with the TEM. NR 36 TC 11 Z9 12 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2010 VL 61 IS 5 BP 1483 EP 1493 DI 10.1093/jxb/erq020 PG 11 WC Plant Sciences SC Plant Sciences GA 569AF UT WOS:000275567500020 PM 20167611 ER PT J AU Elitzur, T Vrebalov, J Giovannoni, JJ Goldschmidt, EE Friedman, H AF Elitzur, Tomer Vrebalov, Julia Giovannoni, James J. Goldschmidt, Eliezer E. Friedman, Haya TI The regulation of MADS-box gene expression during ripening of banana and their regulatory interaction with ethylene SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Developmental control; ethylene; 1-MCP; peel; pulp; ripening ID TRANSCRIPTION FACTOR; FRUIT-DEVELOPMENT; DIFFERENTIAL EXPRESSION; CLIMACTERIC FRUIT; MOLECULAR-BIOLOGY; MUSA-ACUMINATA; FLESHY FRUIT; TOMATO; BIOSYNTHESIS; EVOLUTION AB Six MaMADS-box genes have been cloned from the banana fruit cultivar Grand Nain. The similarity of these genes to tomato LeRIN is low and neither MaMADS2 nor MaMADS1 complement the tomato rin mutation. Nevertheless, the expression patterns, specifically in fruit and the induction during ripening and in response to ethylene and 1-MCP, suggest that some of these genes may participate in ripening. MaMADS1, 2, and 3, are highly expressed in fruit only, while the others are expressed in fruit as well as in other organs. Moreover, the suites of MaMADS-box genes and their temporal expression differ in peel and pulp during ripening. In the pulp, the increase in MaMADS2, 3, 4, and 5 expression preceded an increase in ethylene production, but coincides with the CO(2) peak. However, MaMADS1 expression in pulp coincided with ethylene production, but a massive increase in its expression occurred late during ripening, together with a second wave in the expression of MaMADS2, 3, and 4. In the peel, on the other hand, an increase in expression of MaMADS1, 3, and to a lesser degree also of MaMADS4 and 2 coincided with an increase in ethylene production. Except MaMADS3, which was induced by ethylene in pulp and peel, only MaMADS4, and 5 in pulp and MaMADS1 in peel were induced by ethylene. 1-MCP applied at the onset of the increase in ethylene production, increased the levels of MaMADS4 and MaMADS1 in pulp, while it decreased MaMADS1, 3, 4, and 5 in peel, suggesting that MaMADS4 and MaMADS1 are negatively controlled by ethylene at the onset of ethylene production only in pulp. Only MaMADS2 is neither induced by ethylene nor by 1-MCP, and it is expressed mainly in pulp. Our results suggest that two independent ripening programs are employed in pulp and peel which involve the activation of mainly MaMADS2, 4, and 5 and later on also MaMADS1 in pulp, and mainly MaMADS1, and 3 in peel. Hence, our results are consistent with MaMADS2, a SEP3 homologue, acting in the pulp upstream of the increase in ethylene production similarly to LeMADS-RIN. C1 [Elitzur, Tomer; Friedman, Haya] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-50250 Bet Dagan, Israel. [Vrebalov, Julia; Giovannoni, James J.] ARS, USDA, Ithaca, NY USA. [Vrebalov, Julia; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Elitzur, Tomer; Goldschmidt, Eliezer E.] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Kennedy Leigh Ctr Hort Res, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel. RP Friedman, H (reprint author), Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, POB 6, IL-50250 Bet Dagan, Israel. EM hayafr@agri.gov.il FU Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel [550/09]; BARD (The United States-Israel Binational Agricultural Research and Development Fund) [IS-3803-05] FX Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 550/09. The research was supported by BARD (The United States-Israel Binational Agricultural Research and Development Fund) research Grant Award No. IS-3803-05. NR 64 TC 62 Z9 71 U1 6 U2 26 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2010 VL 61 IS 5 BP 1523 EP 1535 DI 10.1093/jxb/erq017 PG 13 WC Plant Sciences SC Plant Sciences GA 569AF UT WOS:000275567500023 PM 20200120 ER PT J AU Keyser, JD AF Keyser, James D. TI Thunder and Herds: Rock Art of the High Plains SO JOURNAL OF FIELD ARCHAEOLOGY LA English DT Book Review C1 [Keyser, James D.] USDA, Forest Serv, Portland, OR 97239 USA. RP Keyser, JD (reprint author), USDA, Forest Serv, 1815 SW DeWitt, Portland, OR 97239 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU MANEY PUBLISHING PI LEEDS PA STE 1C, JOSEPHS WELL, HANOVER WALK, LEEDS LS3 1AB, W YORKS, ENGLAND SN 0093-4690 J9 J FIELD ARCHAEOL JI J. Field Archaeol. PD MAR PY 2010 VL 35 IS 1 BP 125 EP 126 PG 2 WC Archaeology SC Archaeology GA 626MJ UT WOS:000279969800014 ER PT J AU Zhu, Y Pan, ZL McHugh, TH Barrett, DM AF Zhu, Yi Pan, Zhongli McHugh, Tara H. Barrett, Diane M. TI Processing and quality characteristics of apple slices processed under simultaneous infrared dry-blanching and dehydration with intermittent heating SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Blanching; Partial drying; Apple; Infrared; Intermittent heating ID THERMAL INACTIVATION; POLYPHENOL OXIDASE; ENZYME-ACTIVITY; PEROXIDASE; RADIATION; KINETICS; BANANA; BLUEBERRIES; FRESH AB This study investigated the effects of three processing parameters, e.g. product surface temperature, slice thickness and processing time, on blanching and dehydration characteristics of apple slices exposed to simultaneous infrared dry-blanching and dehydration (SIRDBD) with intermittent heating. A three-factor factorial experiment design was conducted to determine the influence of processing parameters on product temperature, moisture reduction, drying rate, residual polyphenol oxidase (PPO) and peroxidase (POD) activities and surface color change. Slice thickness had a significant effect on product quality and processing characteristics, as faster inactivation of enzymes and quicker moisture reduction took place in thinner slices. A Page model performed well for describing drying behavior during the treatment, and first-order kinetics and a biphasic model fit well for PPO and POD inactivation, respectively. Surface color changes (Delta E) of apple slices during prolonged heating resulted from non-enzymatic browning with an increase in b value was observed. In order to achieve a I log reduction in POD activity, the process resulted in a reduction in moisture from 20% to 59% and in Delta E from 2.27 to 5.59. It is suggested that SIRDBD with intermittent heating could be used as an alternative to manufacture high quality blanched and partially dehydrated fruits and vegetables. (C) 2009 Published by Elsevier Ltd. C1 [Pan, Zhongli; McHugh, Tara H.] ARS, USDA, WRRC, Processed Foods Res Unit, Albany, CA 94710 USA. [Zhu, Yi; Barrett, Diane M.] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP Pan, ZL (reprint author), ARS, USDA, WRRC, Processed Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM zpan@ars.usda.gov NR 34 TC 28 Z9 31 U1 1 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2010 VL 97 IS 1 BP 8 EP 16 DI 10.1016/j.jfoodeng.2009.07.021 PG 9 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 533YR UT WOS:000272862700002 ER PT J AU Guo, WC Zhu, XH Liu, Y Zhuang, H AF Guo, Wenchuan Zhu, Xinhua Liu, Yi Zhuang, Hong TI Sugar and water contents of honey with dielectric property sensing SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Honey solution; Honey adulteration; Total soluble solids content; Water content; Permittivity; Dielectric constant; Dielectric loss factor ID MOISTURE-CONTENT; TEMPERATURE; FREQUENCY; BEHAVIOR; QUALITY; DENSITY; STORAGE; FRUITS; MILK AB The dielectric properties of pure yellow locust, jujube and rape flower honey and their water-adulterated products with water content from 18% to 42.6% were measured with open-ended coaxial-line probe technology and a network analyzer from 10 to 4500 MHz at 25 degrees C. Dielectric constants of pure honeys and water-added honey samples decreased monotonically with increasing frequency, and increased with increasing water content. Dielectric relaxation was evident in the dielectric loss factors. The critical frequency and the maximum loss factor increased with increasing water content. There were strong linear correlations between the dielectric constant and the total soluble solids and water contents. The linear coefficients of determination were higher than 0.995 from 650 to 960 MHz. The good linear correlations and the sufficient penetration depth >20 mm below 960 MHz, suggest that microwave dielectric properties could be used in developing sensors to determine sugar and water contents. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Guo, Wenchuan; Zhu, Xinhua; Liu, Yi] NW A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Zhuang, Hong] ARS, USDA, Russell Res Ctr, Athens, GA 30604 USA. RP Guo, WC (reprint author), NW A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM guowenchuan69@126.com NR 25 TC 26 Z9 30 U1 3 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAR PY 2010 VL 97 IS 2 BP 275 EP 281 DI 10.1016/j.jfoodeng.2009.10.024 PG 7 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 539JH UT WOS:000273249700019 ER PT J AU Valenzuela-Martinez, C Pena-Ramos, A Juneja, VK Korasapati, NR Burson, DE Thippareddi, H AF Valenzuela-Martinez, Carol Pena-Ramos, Aida Juneja, Vijay K. Korasapati, Nageswara Rao Burson, Dennis E. Thippareddi, Harshavardhan TI Inhibition of Clostridium perfringens Spore Germination and Outgrowth by Buffered Vinegar and Lemon Juice Concentrate during Chilling of Ground Turkey Roast Containing Minimal Ingredients SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ORGANIC-ACID SALTS; ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; INJECTED PORK; GROWTH; BEEF; SURVIVAL; PH; TEMPERATURE; PRODUCTS AB Inhibition of Clostridium petfringens spore germination and outgrowth in ground turkey roast containing minimal ingredients (salt and sugar), by buffered vinegar (MOstatin V) and a blend (buffered) of lemon juice concentrate and vinegar (MOstatin LV) was evaluated. Ground turkey roast was formulated to contain sea salt (1.5%), turbinado sugar (0.5%), and various concentrations of MOstatin V (0.75, 1.25, or 2.5%) or MOstatin LV (1.5, 2.5, or 3.5%), along with a control (without MOstatins). The product was inoculated with a three-strain spore cocktail of C. perfringens to obtain initial spore levels of ca. 2.0 to 0.5 log CFU/g. Inoculated products were vacuum packaged, heat shocked for 20 min at 75 degrees C, and cooled exponentially from 54.4 to 4.0 degrees C in 6.5, 9, 12, 15, 18, or 21 h. In control samples without MOstatin V or MOstatin LV, C. petfringens populations reached 2.98, 4.50, 5.78, 7.05, 7.88, and 8.19 log CFU/g (corresponding increases of 0,5 1, 2.29, 3.51, 4.79, 5.55, and 5.93 log CFU/g) in 6.5, 9, 12, 15, 18, and 21 h of chilling, respectively. MOstatin V (2.5%) and MOstatin LV (3.5%) were effective in inhibiting C. perfringens spore germination and outgrowth in ground turkey roast to < 1.0 log CFU/g during abusive chilling of the product within 21 h. Buffered vinegar and a blend (buffered) of lemon juice concentrate and vinegar were effective in controlling germination and outgrowth of C. perfringens spores in turkey roast containing minimal ingredients. C1 [Valenzuela-Martinez, Carol; Korasapati, Nageswara Rao; Thippareddi, Harshavardhan] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. [Burson, Dennis E.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Pena-Ramos, Aida] Ctr Invest Alimentac & Desarrollo AC, Hermosillo 83000, Sonora, Mexico. [Juneja, Vijay K.] ARS, Microbial Food Safety Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Thippareddi, H (reprint author), Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. EM hthippareddi2@unl.edu FU U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service [2004-51110-01889]; University of Nebraska Agricultural Experiment Station FX This work was supported by the National Integrated Food Safety Initiative of the U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service (agreement 2004-51110-01889) and by the University of Nebraska Agricultural Experiment Station. NR 31 TC 13 Z9 13 U1 4 U2 19 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2010 VL 73 IS 3 BP 470 EP 476 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 570LB UT WOS:000275677900008 PM 20202331 ER PT J AU Dorner, JW AF Dorner, Joe W. TI Efficacy of a Biopesticide for Control of Aflatoxins in Corn SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ASPERGILLUS-FLAVUS; BIOLOGICAL-CONTROL; FIELD APPLICATION; UNITED-STATES; PEANUTS; PARASITICUS; CONTAMINATION; BIOCONTROL; POPULATIONS; REDUCTION AB A 2-year study was carried out to determine the efficacy of a biopesticide in reducing aflatoxin contamination in corn. The biopesticide, afla-guard, delivers a nontoxigenic strain of Aspergillus flavus to the field where it competes with naturally occurring toxigenic strains of the fungus. Afla-guard was applied to entire fields in two areas of Texas at either 11.2 or 22.4 kg/ ha. Specific nontreated fields in close proximity to treated fields were designated as controls. Samples of corn were collected at harvest and analyzed for aflatoxins and density of toxigenic and nontoxigenic isolates of A.flavus. Aflatoxin concentrations were generally quite low in 2007, but the mean concentration in treated samples (0.5 ppb) was reduced by 85% compared with controls (3.4 ppb). In 2008, samples from treated and control fields averaged 1.5 and 12.4 ppb, respectively, an 88% reduction. There were no significant differences between the two afla-guard application rates. In conjunction with the reductions in aflatoxin contamination, treatments produced significant reductions in the incidence of toxigenic isolates of A. flavus in corn. C1 ARS, USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. RP Dorner, JW (reprint author), ARS, USDA, Natl Peanut Res Lab, 1011 Forrester Dr SE, Dawson, GA 39842 USA. EM jwdorner@bellsouth.net NR 27 TC 12 Z9 12 U1 0 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2010 VL 73 IS 3 BP 495 EP 499 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 570LB UT WOS:000275677900012 PM 20202335 ER PT J AU Sargeant, JM O'Connor, AM Gardner, IA Dickson, JS Torrence, ME Dohoo, IR Lefebvre, SL Morley, PS Ramirez, A Snedeker, K AF Sargeant, J. M. O'Connor, A. M. Gardner, I. A. Dickson, J. S. Torrence, M. E. Dohoo, I. R. Lefebvre, S. L. Morley, P. S. Ramirez, A. Snedeker, K. TI The REFLECT Statement: Reporting Guidelines for Randomized Controlled Trials in Livestock and Food Safety: Explanation and Elaboration SO JOURNAL OF FOOD PROTECTION LA English DT Review ID RIGHT FLANK LAPAROTOMY; INFECTIOUS BOVINE KERATOCONJUNCTIVITIS; LAPAROSCOPY-GUIDED ABOMASOPEXY; CLINICAL-TRIALS; CONSORT STATEMENT; DAIRY-COWS; SUBGROUP ANALYSES; FIELD TRIAL; INTRAMAMMARY INFECTIONS; REVISED RECOMMENDATIONS AB Concerns about the completeness and accuracy of reporting of randomized clinical trials (RCTs) and the impact of poor reporting on decision-making have been documented in the medical field over the past several decades. Experience from RCTs in human medicine would suggest that failure to report critical trial features can be associated with biased estimated effect measures, and there is evidence to suggest similar biases occur in RCTs conducted in livestock populations. In response to these concerns, standardized guidelines for reporting RCTs were developed and implemented in human medicine. The Consolidated Standards of Reporting Trials (CONSORT) statement was first published in 1996 with a revised edition published in 2001. The CONSORT statement consists of a 22-item checklist for reporting a RCT and a flow diagram to follow the number of participants at each stage of a trial. An explanation and elaboration document not only defines and discusses the importance of each of the items, but also provides examples of how this information could be supplied in a publication. Differences between human and livestock populations necessitate modifications to the CONSORT statement to maximize its usefulness for RCTs involving livestock. These have been addressed in an extension of the CONSORT statement titled the REFLECT statement: Methods and processes of creating reporting guidelines for randomized control trials for livestock and food safety. The modifications made for livestock trials specifically addressed the common use of group housing and group allocation to intervention in livestock studies, the use of a deliberate challenge model in some trials, and common use of non-clinical outcomes, such as contamination with a foodborne pathogen. In addition, the REFLECT statement for RCTs in livestock populations proposed specific terms or further clarified terms as they pertained to livestock studies. C1 [Sargeant, J. M.; Snedeker, K.] Univ Guelph, Ontario Vet Coll, Ctr Publ Hlth & Zoonoses, Guelph, ON N1G 2W1, Canada. [Sargeant, J. M.; Snedeker, K.] Univ Guelph, Ontario Vet Coll, Dept Populat Med, Guelph, ON N1G 2W1, Canada. [O'Connor, A. M.; Ramirez, A.] Iowa State Univ, Vet Med Res Inst, Ames, IA 50011 USA. [Gardner, I. A.] Univ Calif Davis, Sch Vet Med, Dept Med & Epidemiol, Davis, CA 95616 USA. [Dickson, J. S.] Iowa State Univ, Dept Anim Sci, Meat Lab 215F, Ames, IA 50011 USA. [Torrence, M. E.] ARS, USDA, Beltsville, MD 20870 USA. [Dohoo, I. R.] Univ Prince Edward Isl, Atlantic Vet Coll, Dept Hlth Management, Charlottetown, PE C1A 4P3, Canada. [Lefebvre, S. L.] Amer Vet Med Assoc, Schaumburg, IL 60173 USA. [Morley, P. S.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, Ft Collins, CO 80523 USA. RP Sargeant, JM (reprint author), Univ Guelph, Ontario Vet Coll, Ctr Publ Hlth & Zoonoses, Guelph, ON N1G 2W1, Canada. EM sargeanj@uoguelph.ca RI O'Connor, Annette/E-5591-2010; OI O'Connor, Annette/0000-0003-0604-7822; Morley, Paul/0000-0001-8138-2714 FU USDA Food Safety and Response Network [2005-35212-15287]; National Pork Board; Laboratory for Foodborne Zoonoses in the Public Health Agency of Canada; Canadian Institutes of Health Research; Institute of Population and Public Health/Public Health Agency of Canada FX USDA Food Safety and Response Network (Grant 2005-35212-15287); National Pork Board; Laboratory for Foodborne Zoonoses in the Public Health Agency of Canada; Canadian Institutes of Health Research: Institute of Population and Public Health/Public Health Agency of Canada (Applied Public Health Research Chair program); The Association for Veterinary Epidemiology and Preventive Medicine and The American Meat Institute Foundation. NR 114 TC 14 Z9 14 U1 0 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2010 VL 73 IS 3 BP 579 EP 603 PG 25 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 570LB UT WOS:000275677900026 PM 20202349 ER PT J AU Huang, LH Sites, J AF Huang, Lihan Sites, Joseph TI New Automated Microwave Heating Process for Cooking and Pasteurization of Microwaveable Foods Containing Raw Meats SO JOURNAL OF FOOD SCIENCE LA English DT Article DE automatic control; E. coli O157:H7; inverter; microwave ID ESCHERICHIA-COLI O157-H7; HEMORRHAGIC COLITIS; BEEF FRANKFURTERS; CATTLE; PREVALENCE; O157H7; FROZEN AB A new microwave heating process was developed for cooking microwaveable foods containing raw meats. A commercially available inverter-based microwave oven was modified for pasteurization of mechanically tenderized beef, inoculated with Escherichia coli O157:H7 (approximately 5 log(10) CFU/g) and packaged in a 12-oz CPET tray containing 150-mL de-ionized water. The new microwave heating system was equipped with an infrared sensor and a proportional feedback mechanism to allow temperature controlled microwave heating. A 2-stage heating strategy was adopted to cook the product. In the primary heating stage, the sample surface temperature was increased to an initial temperature set-point (ITSP, 65, 70, 75, or 80 degrees C). In the secondary heating stage, the heating was continued with a small fraction of microwave power. The effect of ITSP, hold time (0 to 3 min), and sample elevation (0, 0.03, and 0.07 m above turntable) on inactivation of E. coli O157:H7 and background microflora was evaluated. It was observed that only a small number (approximately 1.3 logs) of E. coli O157:H7 and background microflora were inactivated in the primary heating stage. The elevation 0.07 m, which was in the proximity of the geometric center of the metal cavity, was more effective in inactivating both E. coli O157:H7 and background microflora. Substantially more bacteria were inactivated in the secondary heating stage. Complete inactivation of E. coli and background microflora was observed with heating at temperatures above 70 degrees C for more than 1 min. This study demonstrated a new approach for ensuring the safety of microwaveable products containing raw meats. C1 [Huang, Lihan; Sites, Joseph] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 23 TC 5 Z9 5 U1 1 U2 28 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2010 VL 75 IS 2 BP E110 EP E115 DI 10.1111/j.1750-3841.2009.01482.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 563EI UT WOS:000275110200019 PM 20492230 ER PT J AU Onwulata, CI Phillips, JG Tunick, MH Qi, PX Cooke, PH AF Onwulata, Charles I. Phillips, John G. Tunick, Michael H. Qi, Phoebi X. Cooke, Peter H. TI Texturized Dairy Proteins SO JOURNAL OF FOOD SCIENCE LA English DT Article DE dairy proteins; extrusion; nonfat dried milk; texturization; whey protein concentrate; whey protein isolates ID WHEY PROTEINS; PHYSICAL-PROPERTIES; EXTRUSION; CORN; FUNCTIONALITY; TEMPERATURE; CONCENTRATE; SOLUBILITY; PRODUCTS; POWDERS AB Dairy proteins are amenable to structural modifications induced by high temperature, shear, and moisture; in particular, whey proteins can change conformation to new unfolded states. The change in protein state is a basis for creating new foods. The dairy products, nonfat dried milk (NDM), whey protein concentrate (WPC), and whey protein isolate (WPI) were modified using a twin-screw extruder at melt temperatures of 50, 75, and 100 degrees C, and moistures ranging from 20 to 70 wt%. Viscoelasticity and solubility measurements showed that extrusion temperature was a more significant (P < 0.05) change factor than moisture content. The degree of texturization, or change in protein state, was characterized by solubility (R-2 = 0.98). The consistency of the extruded dairy protein ranged from rigid (2500 N) to soft (2.7 N). Extruding at or above 75 degrees C resulted in increased peak force for WPC (138 to 2500 N) and WPI (2.7 to 147.1 N). NDM was marginally texturized; the presence of lactose interfered with its texturization. WPI products extruded at 50 degrees C were not texturized; their solubility values ranged from 71.8% to 92.6%. A wide possibility exists for creating new foods with texturized dairy proteins due to the extensive range of states achievable. Dairy proteins can be used to boost the protein content in puffed snacksmade from cornmeal, but unmodified, they bind water and form doughy pastes with starch. To minimize the water binding property of dairy proteins, WPI, or WPC, or NDM were modified by extrusion processing. Extrusion temperature conditions were adjusted to 50, 75, or 100 degrees C, sufficient to change the structure of the dairy proteins, but not destroy them. Extrusion modified the structures of these dairy proteins for ease of use in starchy foods to boost nutrient levels. Practical Application: Dairy proteins can be used to boost the protein content in puffed snacks made from corn meal, but unmodified, they bind water and form doughy pastes with starch. To minimize the water binding property of dairy proteins, whey protein isolate, whey protein concentrate, or nonfat dried milk were modified by extrusion processing. Extrusion temperature conditions were adjusted to 50, 75, or 100 degrees C, sufficient to change the structure of the dairy proteins, but not destroy them. Extrusion modified the structures of these dairy proteins for ease of use in starchy foods to boost nutrient levels. C1 [Onwulata, Charles I.; Phillips, John G.; Tunick, Michael H.; Qi, Phoebi X.] ARS, USDA, Eastern Reg Res Ctr, Dairy Proc & Prod Res Unit, Wyndmoor, PA 19038 USA. [Cooke, Peter H.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Onwulata, CI (reprint author), ARS, USDA, Eastern Reg Res Ctr, Dairy Proc & Prod Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.onwulata@ars.usda.gov RI Tunick, Michael/C-9761-2010 NR 34 TC 14 Z9 15 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2010 VL 75 IS 2 BP E100 EP E109 DI 10.1111/j.1750-3841.2009.01473.x PG 10 WC Food Science & Technology SC Food Science & Technology GA 563EI UT WOS:000275110200018 PM 20492229 ER PT J AU Guan, WQ Fan, XT AF Guan, Wenqiang Fan, Xuetong TI Combination of Sodium Chlorite and Calcium Propionate Reduces Enzymatic Browning and Microbial Population of Fresh-Cut "Granny Smith" Apples SO JOURNAL OF FOOD SCIENCE LA English DT Article DE browning; calcium propionate; E. coli; fresh-cut apples; sodium chlorite; yeast and mold ID LISTERIA-MONOCYTOGENES; QUALITY; SLICES; 1-METHYLCYCLOPROPENE; SANITIZERS; INHIBITION; EFFICACY; GROWTH; ACID; PH AB Tissue browning and microbial growth are the main concerns associated with fresh-cut apples. In this study, effects of sodium chlorite (SC) and calcium propionate (CP), individually and combined, on quality and microbial population of apple slices were investigated. "Granny Smith" apple slices, dipped for 5 min in CP solutions at 0%, 0.5%, 1%, and 2% (w/v) either alone or in combination with 0.05% (w/v) SC, were stored at 3 and 10 degrees C for up to 14 d. Color, firmness, and microflora population were measured at 1, 7, and 14 d of storage. Results showed that CP alone had no significant effect on the browning of cut apples. Even though SC significantly inhibited tissue browning initially, the apple slices turned brown during storage at 10 degrees C. The combination of CP and SC was able to inhibit apple browning during storage. Samples treated with the combination of SC with CP did not show any detectable yeast and mold growth during the entire storage period at 3 degrees C. At 10 degrees C, yeast and mold count increased on apple slices during storage while CP reduced the increase. However, high concentrations of CP reduced the efficacy of SC in inactivating E. coli inoculated on apples. Overall, our results suggested that combination of SC with 0.5% and 1% CP could be used to inhibit tissue browning and maintain firmness while reducing microbial population. Practical Application: Apple slices, which contain antioxidants and other nutrient components, have emerged as popular snacks in food service establishments, school lunch programs, and for family consumption. However, the further growth of the industry is limited by product quality deterioration caused by tissue browning, short shelf-life due to microbial growth, and possible contamination with human pathogens during processing. Therefore, this study was conducted to develop treatments to reduce microbial population and tissue browning of "Granny Smith" apple slices. Results showed that an antimicrobial compound, sodium chlorite, is effective in not only eliminating microbes but also inhibiting tissue browning of apple slices. However, the compound caused tissue softening and its antibrowning effect was short-lived, lasting only for a few days. Combination of the compound with a calcium-containing food additive was able to improve firmness and freshness of apple slices while reducing population of Escherichia coli artificially inoculated on samples and inhibiting the growth of yeast and mold during storage. C1 [Fan, Xuetong] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Guan, Wenqiang] Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov NR 24 TC 8 Z9 8 U1 1 U2 22 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2010 VL 75 IS 2 BP M72 EP M77 DI 10.1111/j.1750-3841.2009.01470.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 563EI UT WOS:000275110200024 PM 20492244 ER PT J AU Jin, T AF Jin, Tony TI Inactivation of Listeria monocytogenes in Skim Milk and Liquid Egg White by Antimicrobial Bottle Coating with Polylactic Acid and Nisin SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antimicrobial bottle coating; L. monocytogenes; liquid egg; milk; nisin; PLA ID WHOLE EGG; PASTEURIZED MILK; QUALITY CHANGES; POLYMER-FILMS; INHIBITION; FORMULATION; SALMONELLA; OUTBREAK; ALGINATE; SURFACE AB This study was to develop an antimicrobial bottle coating method to reduce the risk of outbreaks of human listeriosis caused by contaminated liquid foods. Liquid egg white and skim milk were inoculated with Listeria monocytogenes Scott A and stored in glass jars that were coated with a mixture of polylactic acid (PLA) polymer and nisin. The efficacy of PLA per nisin coating in inactivating L. monocytogenes was investigated at 10 and 4 degrees C. The pathogen grew well in skim milk without PLA/nisin coating treatments, reaching 8 log CFU/mL after 10 d and then remained constant up to 42 d at 10 degrees C. The growth of Listeria at 4 degrees C was slower than that at 10 degrees C, taking 21 d to obtain 8 log CFU/mL. At both storage temperatures, the PLA coating with 250mg nisin completely inactivated the cells of L. monocytogenes after 3 d and throughout the 42-d storage period. In liquid egg white, Listeria cells in control and PLA coating without nisin samples declined 1 log CFU/mL during the first 6 d at 10 degrees C and during 28 d at 4 degrees C, and then increased to 8 or 5.5 log CFU/mL. The treatment of PLA coating with 250 mg nisin rapidly reduced the cell numbers of Listeria in liquid egg white to undetectable levels after 1 d, then remained undetectable throughout the 48 d storage period at 10 degrees C and the 70 d storage period at 4 degrees C. These data suggested that the PLA/nisin coating treatments effectively inactivated the cells of L. monocytogenes in liquid egg white and skim milk samples at both 10 and 4 degrees C. This study demonstrated the commercial potential of applying the antimicrobial bottle coating method to milk, liquid eggs, and possibly other fluid products. C1 ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Jin, T (reprint author), ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 NR 54 TC 9 Z9 11 U1 1 U2 12 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2010 VL 75 IS 2 BP M83 EP M88 DI 10.1111/j.1750-3841.2009.01480.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 563EI UT WOS:000275110200026 PM 20492246 ER PT J AU Stein, SM McRoberts, RE Nelson, MD Mahal, L Flather, CH Alig, RJ Comas, S AF Stein, Susan M. McRoberts, Ronald E. Nelson, Mark D. Mahal, Lisa Flather, Curtis H. Alig, Ralph J. Comas, Sara TI Private Forest Habitat for At-Risk Species: Where Is It and Where Might It Be Changing? SO JOURNAL OF FORESTRY LA English DT Article DE private forest; at-risk species; housing development; corporate forest ID CONTERMINOUS UNITED-STATES; LAND; URBANIZATION; RICHNESS; AREAS; USA AB Owned by individuals, families, corporations, and other private groups, private forests account for almost 60% of all forestland in the conterminous United States and provide critical habitat for wildlife, including over 3,700 at-risk plant and animal species. Extensive areas of private forest and other land covers have experienced increased housing development in recent years, with numerous implications for wildlife conservation. Twenty-eight percent of all private forests are under corporate ownership and provide important wildlife habitat. In some areas of the country, large amounts of private forest under corporate ownership are being sold and, in some cases, subdivided, with consequent implications for at-risk species conservation. Sponsored by the US Forest Service, the Forests on the Edge (FOTE) project uses geographic information systems to identify areas across the United States where private forests provide important services that might be detrimentally affected by increased housing and other threats. This article presents the results of FOTE research on the importance of private forests in general, and corporate forests in particular, to at-risk species. It also identifies areas across the country where future housing development on private forests could further reduce wildlife habitat. Results indicate that private forests and private corporate forests in the West Coast states, parts of the Gulf and Atlantic Coasts, as well as interior areas of the Southeast provide habitat for a large number of at-risk species and that these areas are also among those where private forests are most likely to experience increased housing development. C1 [Stein, Susan M.; Comas, Sara] US Forest Serv, Cooperat Forestry Staff, Washington, DC 20250 USA. [McRoberts, Ronald E.; Nelson, Mark D.; Mahal, Lisa] US Forest Serv, N Cent Res Stn, St Paul, MN 55108 USA. [Flather, Curtis H.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Alig, Ralph J.] US Forest Serv, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. RP Stein, SM (reprint author), US Forest Serv, Cooperat Forestry Staff, Mail Stop 1123,1400 Independence Ave SW, Washington, DC 20250 USA. EM sstein@fs.fed.us; rmcroberts@fs.fed.us; mdnelson@fs.fed.us; lmahal@fs.fed.us; cflather@fs.fed.us; ralig@fs.fed.us; scomas@fs.fed.us RI Flather, Curtis/G-3577-2012 OI Flather, Curtis/0000-0002-0623-3126 FU Forest Service, State and Private Forestry, Cooperative Forestry Staff as well as Assistant Director Ted Beauvais FX Susan M Stein (sstein@fs fed.us) is private forest/and studies coordinator and Susan Comas (scomas@fs.fed.us) is natural resource specialist, US Forest Service Cooperative Forestry Staff Mail Stop 1123, 1400 Independence Ave. SW Washington, DC 20250-1123. Ronald E. McRoberts (rmcroberts@fs.fed.us) is mathematical statistician, Mark D. Nelson (mdnelson@fs.fed.us) is research forester, and Lisa Mahal (lmahal@fs.fed.us) is geographic information systems analyst, US Forest Service, North Central Research Station, 1992 Falwell Ave., St. Paul, MN 55108. Curtis H. Flather (cflather@fs.fed.us) is research wildlife biologist, US Forest Service, Rocky Mountain Research Station, 2150 Centre Ave., Bldg. A, Fort Collins, CO 80526-1891. Ralph J. Alig(ralig@fs.fed.us) is research forester, US Forest Service, Pacific Northwest Research Station, Forestry Sciences Laboratory, 3200 SW Jefferson Way, Corvallis, OR 07331. The authors acknowledge Larry Payne, recent Director of the Forest Service, State and Private Forestry, Cooperative Forestry Staff as well as Assistant Director Ted Beauvais, for their inspiration for and support of the Forests on the Edge project. NR 34 TC 1 Z9 2 U1 0 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2010 VL 108 IS 2 BP 61 EP 70 PG 10 WC Forestry SC Forestry GA 575TL UT WOS:000276091200006 ER PT J AU Carroll, MS Cohn, PJ Paveglio, TB Drader, DR Jakes, PJ AF Carroll, Matthew S. Cohn, Patricia J. Paveglio, Travis B. Drader, Donna R. Jakes, Pamela J. TI Fire Burners to Firefighters: The Nez Perce and Fire SO JOURNAL OF FORESTRY LA English DT Article DE fire policy; Nez Perce; indigenous burning ID UNITED-STATES; NORTHWEST AB This article presents results from an interview-based case study examining burning practices of the Nez Perce tribe in the Inland Northwest in both their contemporary and historical policy context. Despite the lack of a prominent fire tradition, our interviews uncovered a legacy of knowledge and cultural traditions linked to fire and a variety of contemporary fire practices on the reservation performed by land-management professionals and individual tribal members. Many of these practices, particularly those involving broadcast burning, have diminished over the years. We examine the reasons for this and the potentials for mitigating some of the practical and policy constraints to such burning. We conclude that the nontribal community still has much to learn about fire from those who have lived in fire-adapted landscapes longer than anyone else. C1 [Carroll, Matthew S.; Cohn, Patricia J.; Paveglio, Travis B.; Drader, Donna R.] Washington State Univ, Dept Nat Resource Sci, Pullman, WA 99164 USA. [Jakes, Pamela J.] US Forest Serv, No Res Stn, St Paul, MN USA. RP Carroll, MS (reprint author), Washington State Univ, Dept Nat Resource Sci, Pullman, WA 99164 USA. EM carroll@wsu.edu; pcohn@wsu.edu; travispaveglio@wsu.edu; dlorincz@wsu.edu; pjakes@fs.fed.us FU National Fire Plan through the Northern Research Station; US Forest Service; St. Paul, MN; Agricultural Research Center; Washington State University, Pullman, WA FX Matthew S. Carroll (carroll@wsu.edu) is professor, Patricia J. Cohn (pcohn@wsu.edu), Travis B. Paveglio (travispaveglio@wsu.edu) is PhD candidate, and Donna R. Drader (dlorincz@wsu.edu) is firmer master's student, Department of Natural Resource Sciences, Washington State University, Pullman, WA. Pamela J. lakes (pjakes@fi.fid.us) is research forester, US Forest Service, Northern Research Station, St. Paul, MN This research was supported by the National Fire Plan through the Northern Research Station, US Forest Service, St. Paul, MN, and the Agricultural Research Center, Washington State University, Pullman, WA. The authors wish to acknowledge assistance of Allan Marshall of Lewis Clark College, Lewiston, ID, and John DeGroot and Josiah Pinkham of the Nez Perce Tribe and all the tribal members who kindly agreed to participate. These individuals are not responsible, however, for the interpretations of the data made in this article. NR 31 TC 12 Z9 12 U1 1 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD MAR PY 2010 VL 108 IS 2 BP 71 EP 76 PG 6 WC Forestry SC Forestry GA 575TL UT WOS:000276091200007 ER PT J AU Winter, PL Burn, SM AF Winter, Patricia L. Burn, Shawn M. TI Fostering Sustainable Operations in a Natural Resource Management Agency: Insights from the Field SO JOURNAL OF FORESTRY LA English DT Article DE sustainable operations; environmentally responsible actions; Proenvironmental Behavior Change Model; successful implementation ID ORGANIZATIONS; ENVIRONMENT AB Sustainable operations (SO; operating in an environmentally, economically, and socially sustainable manner) is consistent with the environmental stewardship mission of natural resource management organizations. This study sought to examine SO practices in the daily work lives of US Forest Service employees, including those primarily stationed in the office and in the field. The purpose was to identify influences on these behaviors such that organizations can more effectively promote them. We surveyed a random sample of employees within a region and research station of the US Forest Service (n = 451) regarding SO behaviors, barriers, and facilitators to SO, and perspectives on SO. Consistent with the Proenvironmental Behavior Change Model (Burn, S.M. and P.L. Winters. 2008, A behavioral intervention tool for recreation managers, Park Sri. 31[1]:5-15), social norms, attitudes, setting design, knowledge and information, and habit were all important influences on SO behaviors, with social norms, attitudes, and habits the strongest influences. Recommendations for promoting SO are provided. C1 [Winter, Patricia L.] US Forest Serv, Pacific SW Res Stn, Riverside, CA 92507 USA. [Burn, Shawn M.] Calif Polytech State Univ San Luis Obispo, San Luis Obispo, CA 93407 USA. RP Winter, PL (reprint author), US Forest Serv, Pacific SW Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM pwinter@fs.fed.us; sburn@calpoly.edu NR 27 TC 1 Z9 1 U1 1 U2 7 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD MAR PY 2010 VL 108 IS 2 BP 86 EP 92 PG 7 WC Forestry SC Forestry GA 575TL UT WOS:000276091200009 ER PT J AU Kimbell, AR Hickman, C Brown, H AF Kimbell, Abigail R. Hickman, Cliff Brown, Hutch TI How Do Taxes Affect America's Private Forestland Owners? SO JOURNAL OF FORESTRY LA English DT Editorial Material ID VALUES; SOUTH C1 [Kimbell, Abigail R.; Hickman, Cliff; Brown, Hutch] US Forest Serv, Washington, DC 20250 USA. RP Kimbell, AR (reprint author), US Forest Serv, Washington, DC 20250 USA. NR 34 TC 2 Z9 2 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD MAR PY 2010 VL 108 IS 2 BP 93 EP 97 PG 5 WC Forestry SC Forestry GA 575TL UT WOS:000276091200010 ER PT J AU Deal, RL AF Deal, Robert L. TI Climate Change and Carbon Sequestration Opportunities on National Forests SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 US Forest Serv, PNW Res Stn, Portland, OR 97205 USA. RP Deal, RL (reprint author), US Forest Serv, PNW Res Stn, 620 SW Main St, Portland, OR 97205 USA. EM rdeal@fs.fed.us NR 2 TC 0 Z9 0 U1 0 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD MAR PY 2010 VL 108 IS 2 BP 103 EP 103 PG 1 WC Forestry SC Forestry GA 575TL UT WOS:000276091200015 ER PT J AU Spigler, RB Lewers, KS Johnson, AL Ashman, TL AF Spigler, Rachel B. Lewers, Kim S. Johnson, Anna L. Ashman, Tia-Lynn TI Comparative Mapping Reveals Autosomal Origin of Sex Chromosome in Octoploid Fragaria virginiana SO JOURNAL OF HEREDITY LA English DT Article DE allopolypoidy; dioecy; Fragaria; male sterility; sex chromosome evolution ID HYBRID MALE-STERILITY; Y-CHROMOSOME; DIPLOID FRAGARIA; POLYPLOID FORMATION; WILD STRAWBERRY; X ANANASSA; POLYMORPHIC MICROSATELLITES; INDEPENDENT ORIGIN; DNA-SEQUENCES; LINKAGE MAPS AB Recent evolution of separate sexes in flowering plants provides unparalleled opportunities for understanding the early stages of sex chromosome evolution, including their origin from autosomes. Moreover, the transition from combined to separate sexes can be associated with speciation via polyploidization in angiosperms, suggesting that genome doubling/merger may facilitate sterility mutations required for sex chromosome formation. To gain insight into the origin of sex chromosomes in a polyploid plant, we doubled the simple sequence repeat (SSR) density and increased genome coverage in a genetic map of octoploid Fragaria virginiana, a species purported to have a "proto-sex" chromosome, where limited recombination occurs between 2 linked "loci" carrying the male-and female-sterility mutations. Incorporation of almost 3 times the number of SSR markers into the current map facilitated complete characterization of the F. virginiana proto-sex chromosome, revealing its largely autosomal nature and the location of the sex-determining region toward the distal end. Furthermore, extensive synteny between our genetic map and a map involving diploid hermaphroditic congeners allowed assignment of linkage groups to homeologous groups, identification of the proto-sex chromosome's autosomal homoeolog, and detection of a putative rearrangement near the sex-determining region. Fine mapping and additional comparative work will shed light on the intriguing possibility that rearrangements during polyploidization were involved in the evolution of sex chromosomes in Fragaria. C1 [Spigler, Rachel B.; Johnson, Anna L.; Ashman, Tia-Lynn] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA. [Lewers, Kim S.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. RP Ashman, TL (reprint author), Univ Pittsburgh, Dept Biol Sci, 4249 5th Ave, Pittsburgh, PA 15260 USA. EM tia1@pitt.edu FU Cooperative State Research, Education, and Extension Service National Research Initiative of the United States Department of Agriculture [2005-00765]; National Science Foundation [DEB-0449488] FX Cooperative State Research, Education, and Extension Service National Research Initiative of the United States Department of Agriculture (2005-00765); National Science Foundation (DEB-0449488). NR 81 TC 29 Z9 29 U1 2 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 J9 J HERED JI J. Hered. PD MAR-APR PY 2010 VL 101 SU 1 BP S107 EP S117 DI 10.1093/jhered/esq001 PG 11 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 653EJ UT WOS:000282070600014 PM 20421320 ER PT J AU Stewart, D Canfield, E Yitayew, M Nichols, M AF Stewart, Dave Canfield, Evan Yitayew, Muluneh Nichols, Mary TI Estimating an Impedance-to-Flow Parameter for Flood Peak Prediction in Semiarid Watersheds SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Hydrologic models; Travel time; Hydraulic roughness; Peak flow; Impedance to flow ID TIME AB The time of concentration equation used in Pima County, Arizona, includes a hydrologic parameter representing the impedance to flow for peak discharge estimation on small [typically less than 2.59 km(2) (1 mi(2))] semiarid watersheds. The impedance-to-flow parameter is similar in function to the hydraulic Manning's n roughness coefficient in the kinematic wave time of concentration equation; however, the impedance to flow is a hydrologic parameter representing all portions of a watershed rather than a hydraulic parameter representing friction loss during uniform flow. To relate the impedance-to-flow parameter to physical watershed characteristics, impedance-to-flow values were calculated for return period and observed events on five undeveloped rangeland watersheds and correlated with Manning's n roughness coefficients determined from particle size analysis and simulated flow conditions. Impedance to flow displayed a positive trend with observed peak discharge on each watershed. The results indicate that local impedance-to-flow values can be developed for time of concentration equations using observed rainfall and runoff data, as well as measurable field characteristics. The impedance-to-flow parameter allows for a physical basis in time of concentration estimation without the additional detail of a physically based model. C1 [Stewart, Dave; Canfield, Evan] Pima Cty Reg Flood Control Dist, Tucson, AZ 85701 USA. [Yitayew, Muluneh] Univ Arizona, Tucson, AZ 85721 USA. [Nichols, Mary] ARS Res, USDA, Tucson, AZ 85719 USA. RP Stewart, D (reprint author), Pima Cty Reg Flood Control Dist, 97 E Congress St, Tucson, AZ 85701 USA. EM dave.stewart@rfcd.pima.gov NR 28 TC 0 Z9 0 U1 1 U2 2 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 J9 J HYDROL ENG JI J. Hydrol. Eng. PD MAR PY 2010 VL 15 IS 3 BP 182 EP 190 DI 10.1061/(ASCE)HE.1943-5584.0000177 PG 9 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 555NH UT WOS:000274518200003 ER PT J AU Taylor, RAJ Bauer, LS Poland, TM Windell, KN AF Taylor, R. A. J. Bauer, Leah S. Poland, Therese M. Windell, Keith N. TI Flight Performance of Agrilus planipennis (Coleoptera: Buprestidae) on a Flight Mill and in Free Flight SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Dispersal; emerald ash borer; flight endurance; flight speed; invasive species; migration; range expansion ID EMERALD ASH BORER; TETHERED FLIGHT; NATURAL ENEMIES; NORTH-AMERICAN; BEHAVIOR; LEAFHOPPERS; LEPIDOPTERA; NOCTUIDAE; DISTANCE; DENSITY AB Computer-monitored flight mills were used to record the flight performance of the emerald ash borer, Agrilus planipennis Fairmaire. Flight performance of fed and unfed, and mated and unmated beetles of both sexes were recorded and compared. Mated females flew further per day and longer than unmated females or males. Mated females that were allowed to feed between flight periods flew an average of 1.3 km/day for four days; 10% flew more than 7 km/day. Measurements of free-flight speed using a mirror and a high-speed camera were used to calibrate the flight mill results, permitting absolute estimates of flight performance to be made. Free-flight speeds were approximately three times the speeds recorded by the flight mills. The median corrected distance flown by mated females was > 3 km with 20% flying > 10 km and 1% flying > 20 km. The flight performance of mated females suggests a considerable capacity for range expansion by this invasive species. The implications for quarantine and control are discussed. C1 [Taylor, R. A. J.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Bauer, Leah S.; Poland, Therese M.] USDA FS, No Res Stn, E Lansing, MI USA. [Windell, Keith N.] USDA FS, Technol & Dev Ctr, Missoula, MT USA. RP Taylor, RAJ (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. EM RAJT@osu.edu FU USDA Forest Service Northern Research Station (NRS) [03-JV-11231200-097]; Forest Health Technology Enterprise Team (FHTET); Ohio State University FX This research was conducted in part with funding from the USDA Forest Service Northern Research Station (NRS) (formerly North Central Research Station) (grant 03-JV-11231200-097), Forest Health Technology Enterprise Team (FHTET), and The Ohio State University. We thank Drs. Joseph Charney and Robert Haack, USDA-FS NRS, and Frank Sapio, USDA-FS FHTET for assistance in acquiring funding for this RJVA; Deborah L. Miller and Christopher (Kit) O'Connor for assistance with handling, rearing, and flying EAB on the flight mills; Erin Clark for assistance with the free flight experiments; Dr. James Weinkam for reviewing the free flight calculations; and Drs. Larry Phelan, Noah Koller, and Mike Ulyshen for their helpful, careful, and critical reviews of this manuscript. NR 49 TC 54 Z9 59 U1 7 U2 66 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 J9 J INSECT BEHAV JI J. Insect Behav. PD MAR PY 2010 VL 23 IS 2 BP 128 EP 148 DI 10.1007/s10905-010-9202-3 PG 21 WC Entomology SC Entomology GA 553YL UT WOS:000274402600005 ER PT J AU Shrestha, S Park, Y Stanley, D Kim, Y AF Shrestha, Sony Park, Yoonseong Stanley, David Kim, Yonggyun TI Genes encoding phospholipases A(2) mediate insect nodulation reactions to bacterial challenge SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Immunity; Eicosanoid; Phospholipase A(2); Tribolium castaneum ID TOBACCO HORNWORM; MANDUCA-SEXTA; SPODOPTERA-EXIGUA; XENORHABDUS-NEMATOPHILUS; EICOSANOIDS; IMMUNITY; INFECTION; HEMOCYTES; RESPONSES; PLA(2) AB We propose that expression of four genes encoding secretory phospholipases A(2) (sPLA(2)) mediates insect nodulation responses to bacterial infection. Nodulation is the quantitatively predominant cellular defense reaction to bacterial infection. This reaction is mediated by eicosanoids, the biosynthesis of which depends on PLA(2)-catalyzed hydrolysis of arachidonic acid (AA) from cellular phospholipids. Injecting late instar larvae of the red flour beetle, Tribolium castaneum, with the bacterium, Escherichia coli, stimulated nodulation reactions and sPLA(2) activity in time- and dose-related manners. Nodulation was inhibited by pharmaceutical inhibitors of enzymes involved in eicosanoid biosynthesis, and the inhibition was rescued by AA. We cloned five genes encoding sPLA(2) and expressed them in E. coli cells to demonstrate these genes encode catalytically active sPLA(2)s. The recombinant sPLA(2)s were inhibited by sPLA(2) inhibitors. Injecting larvae with double-stranded RNAs specific to each of the five genes led to reduced expression of the corresponding sPLA(2) genes and to reduced nodulation reactions to bacterial infections for four of the five genes. The reduced nodulation was rescued by AA, indicating that expression of four genes encoding sPLA(2)s mediates nodulation reactions. A polyclonal antibody that reacted with all five sPLA(2)s showed the presence of the sPLA(2) enzymes in hemocytes and revealed that the enzymes were more closely associated with hemocyte plasma membranes following infection. Identifying specific sPLA(2) genes that mediate nodulation reactions strongly supports our hypothesis that sPLA(2)s are central enzymes in insect cellular immune reactions. (C) 2009 Elsevier Ltd. All rights reserved. C1 [Shrestha, Sony; Kim, Yonggyun] Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. [Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Kim, Y (reprint author), Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. EM hosanna@andong.ac.kr RI Park, Yoonseong/J-5861-2013 OI Park, Yoonseong/0000-0003-1191-7335 FU Rural Development Administration; Ministry of Education, Science and Technology, Korea FX Most of this work was supported by AGEND 2009 research program of Rural Development Administration. S. Shrestha was supported by the second stage BK21 program of the Ministry of Education, Science and Technology, Korea. This article reports the results of research only and mention of a proprietary product does not constitute an endorsement or recommendation for its use by the USDA. NR 29 TC 24 Z9 26 U1 2 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAR PY 2010 VL 56 IS 3 BP 324 EP 332 DI 10.1016/j.jinsphys.2009.11.008 PG 9 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 572AX UT WOS:000275799700014 PM 19931277 ER PT J AU Vega-Aquino, P Sanchez-Pena, S Blanco, CA AF Vega-Aquino, Paulina Sanchez-Pena, Sergio Blanco, Carlos A. TI Activity of oil-formulated conidia of the fungal entomopathogens Nomuraea rileyi and Isaria tenuipes against lepidopterous larvae SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Entomopathogen; Ascomycota; Nomuraea; Isaria; Oil formulation; Lepidoptera; Spodoptera; Heliothis; Helicoverpa; Infection; Larva ID METARHIZIUM-FLAVOVIRIDE; VELVETBEAN CATERPILLAR; SUSCEPTIBILITY; TEMPERATURE; INFECTIVITY; NOCTUIDAE; PATHOGENS; INSECT; LOOPER; MEXICO AB The fungi Nomuraea rileyi and Isaria tenuipes (=Paecilomyces tenuipes) are ecologically obligate, widespread pathogens of lepidopterans. Bioassays were carried out to evaluate the activity of oil-suspended conidia of N. rileyi and I. tenuipes against larvae of Spodoptera frugiperda, Spodoptera exigua, Helicoverpa zea, and Heliothis virescens. The tests consisted of two bioassay sets. In the first set, conidia of N. rileyi and I. tenuipes were suspended in water + Tween 80, and in vegetable (canola, soybean) and mineral (proprietary mixture of alkanes and cyclic paraffins) oils, and tested against S. frugiperda. Both fungi were highly compatible with oils and caused mortalities near 100% in all oil treatments; the lowest LT(50) values were 4.7 days for N. rileyi in mineral oil and 6.0 days for I. tenuipes in soybean oil. The second set included additional fungal strains and oil formulations (mineral, canola, sunflower, olive and peanut oils) tested against larvae of S. exigua, S. frugiperda, H. zea and H. virescens. The highest activity was that of N. rileyi in mineral oil against Spodoptera spp., with LT(50) values of 2.5 days (strain ARSEF 135) and 3 days (strain ARSEF 762) respectively. For two different isolates of I. tenuipes the lowest LT(50) values (5.1-5.6 days respectively) were obtained with mineral oil formulations against Spodoptera spp. and H. zea respectively. Additionally, we tested both fungi against prepupae of all four lepidopteran species. Mortalities with I. tenuipes against S. exigua ranged from 90% to 100% (strains ARSEF 2488 and 4096); N. rileyi caused 95% mortality on S. frugiperda. The activity of formulations depended on host species and oil used; Spodoptera spp. was more susceptible to these fungi than Heliothis and Helicoverpa. The results indicate that a comprehensive evaluation of these entomopathogens in agriculture using oil application technologies is advisable, particularly, in organic and sustainable settings. (C) 2009 Elsevier Inc. All rights reserved. C1 [Vega-Aquino, Paulina; Sanchez-Pena, Sergio] Univ Autonoma Agr Antonio Narro, Dept Parasitol, Saltillo 25315, Coahuila, Mexico. [Blanco, Carlos A.] USDA ARS, So Insect Management Res Unit, Stoneville, MS 38776 USA. RP Sanchez-Pena, S (reprint author), Univ Autonoma Agr Antonio Narro, Dept Parasitol, Saltillo 25315, Coahuila, Mexico. EM pvega83@gmail.com; sanchezcheco@gmail.com; carlos.blanco@ars.usda.gov NR 24 TC 9 Z9 10 U1 3 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2010 VL 103 IS 3 BP 145 EP 149 DI 10.1016/j.jip.2009.12.002 PG 5 WC Zoology SC Zoology GA 561OO UT WOS:000274987600001 PM 20025883 ER PT J AU Wraight, SP Ramos, ME Avery, PB Jaronski, ST Vandenberg, JD AF Wraight, S. P. Ramos, M. E. Avery, P. B. Jaronski, S. T. Vandenberg, J. D. TI Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Beauveria bassiana; Virulence; Lepidoptera; Larvae; Bioassay; Bioassay statistics ID VARIABLES; NORMALITY; VARIANCE; POWERFUL; RATIOS; TESTS AB Forty-three isolates of the entomopathogenic fungus Beauveria bassiana were screened for virulence against second-instar larvae of diamondback moth (Plutella xylostella) (DBM), European corn borer (Ostrinia nubilalis) (ECB), corn earworm (Helicoverpa zea) (CEW), and fall armyworm (Spodoptera frugiperda) (FAW); 30 of these isolates were tested against beet armyworm (Spodoptera exigua) (BAW). Highly virulent isolates were also tested against black cutworm (Agrotis ipsilon) (BCW), and the most virulent isolate was also assayed against imported cabbage worm (Pieris rapae) (ICW) and cabbage looper (Trichoplusia ni) (CL). All lepidopteran species tested were susceptible to B. bassiana. Corn earworm and beet armyworm were most susceptible to fungal infection, and fall armyworm was least susceptible. Limited testing suggested low susceptibility of black cutworm and cabbage looper. B. bassiana isolate 1200 exhibited virulence against all pest species greater than or equal to commercial strain GHA of B. bassiana currently registered in the USA as BotaniGard (R). In assays in which larvae were topically sprayed and maintained on the treated substrate for 24 h at 100% relative humidity, 6-day (25 degrees C) median lethal concentrations (LC(50)s) of this isolate against CEW, BAW, DBM, FAW, ICW, ECB, CL, and BCW were 4, 5, 7, 11, 12. 98, 125, and 273 conidia/mm(2), respectively. The respective LC(50)s of commercial strain GHA against these pest species were 9, 67, 97, 1213. 29, 1668, 541, and 3504 conidia/mm(2). Use of LC(50) versus median lethal concentration ratios (comparing LC(50)s of each isolate to a "standard" strain) generated similar rankings of isolate virulence. Results from parametric ANOVAs of log LC(50) values followed by Tukey HSD multiple comparisons tests and those from Kruskal-Wallis nonparametric analyses followed by sequential Bonferroni tests for means comparisons were nearly identical. Published by Elsevier Inc. C1 [Wraight, S. P.; Ramos, M. E.; Vandenberg, J. D.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14850 USA. [Avery, P. B.] Indian River Res & Educ Ctr, IFAS, UF, Ft Pierce, FL 34945 USA. [Jaronski, S. T.] Mycotech Corp, Butte, MT 59701 USA. RP Wraight, SP (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, Tower Road, Ithaca, NY 14850 USA. EM spw4@cornell.edu RI Jaronski, Stefan/E-8507-2012 OI Jaronski, Stefan/0000-0002-7789-0406 NR 35 TC 30 Z9 37 U1 2 U2 39 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2010 VL 103 IS 3 BP 186 EP 199 DI 10.1016/j.jip.2010.01.001 PG 14 WC Zoology SC Zoology GA 561OO UT WOS:000274987600007 PM 20060396 ER PT J AU Devinoy, E Rijnkels, M AF Devinoy, Eve Rijnkels, Monique TI Epigenetics in Mammary Gland Biology and Cancer SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Editorial Material ID INHERITANCE; HUMANS C1 [Devinoy, Eve] INRA, UR1196, F-78352 Jouy En Josas, France. [Rijnkels, Monique] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Devinoy, E (reprint author), INRA, UR1196, F-78352 Jouy En Josas, France. EM eve.devinoy@jouy.inra.fr; rijnkel@bcm.tmc.edu NR 16 TC 7 Z9 7 U1 0 U2 3 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD MAR PY 2010 VL 15 IS 1 SI SI BP 1 EP 4 DI 10.1007/s10911-010-9171-3 PG 4 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 557FZ UT WOS:000274656300001 PM 20148294 ER PT J AU Kress, C Ballester, M Devinoy, E Rijnkels, M AF Kress, Clemence Ballester, Maria Devinoy, Eve Rijnkels, Monique TI Epigenetic Modifications in 3D: Nuclear Organization of the Differentiating Mammary Epithelial Cell SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Article DE Mammary gland; Nuclear organization; Epigenetics; Chromatin; Development ID CHROMOSOME TERRITORY ORGANIZATION; SCALE CHROMATIN REORGANIZATION; ACIDIC PROTEIN GENE; ART. NO. 44; EXTRACELLULAR-MATRIX; ACTIVE GENES; CONSTITUTIVE HETEROCHROMATIN; INTERPHASE CHROMOSOMES; SPATIAL-ORGANIZATION; HISTONE ACETYLATION AB During the development of tissues, complex programs take place to reach terminally differentiated states with specific gene expression profiles. Epigenetic regulations such as histone modifications and chromatin condensation have been implicated in the short and long-term control of transcription. It has recently been shown that the 3D spatial organization of chromosomes in the nucleus also plays a role in genome function. Indeed, the eukaryotic interphase nucleus contains sub-domains that are characterized by their enrichment in specific factors such as RNA Polymerase II, splicing machineries or heterochromatin proteins which render portions of the genome differentially permissive to gene expression. The positioning of individual genes relative to these sub-domains is thought to participate in the control of gene expression as an epigenetic mechanism acting in the nuclear space. Here, we review what is known about the sub-nuclear organization of mammary epithelial cells in connection with gene expression and epigenetics. Throughout differentiation, global changes in nuclear architecture occur, notably with respect to heterochromatin distribution. The positions of mammary-specific genes relative to nuclear sub-compartments varies in response to hormonal stimulation. The contribution of tissue architecture to cell differentiation in the mammary gland is also seen at the level of nuclear organization, which is sensitive to microenvironmental stimuli such as extracellular matrix signaling. In addition, alterations in nuclear organization are concomitant with immortalization and carcinogenesis. Thus, the fate of cells appears to be controlled by complex pathways connecting external signal integration, gene expression, epigenetic modifications and chromatin organization in the nucleus. C1 [Kress, Clemence; Devinoy, Eve] INRA, Genom & Physiol Lactat UR1196, F-78352 Jouy En Josas, France. [Ballester, Maria] UAB, IRTA, CReSA, Barcelona 08193, Spain. [Rijnkels, Monique] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Kress, C (reprint author), INRA, Genom & Physiol Lactat UR1196, F-78352 Jouy En Josas, France. EM Clemence.Kress@jouy.inra.fr RI Ballester, Maria/H-7277-2013; Kress, Clemence/M-7286-2014; Ballester, Maria/E-7201-2016 OI Ballester, Maria/0000-0002-5413-4640 NR 85 TC 10 Z9 11 U1 0 U2 5 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD MAR PY 2010 VL 15 IS 1 SI SI BP 73 EP 83 DI 10.1007/s10911-010-9169-x PG 11 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 557FZ UT WOS:000274656300007 PM 20143138 ER PT J AU Rijnkels, M Kabotyanski, E Montazer-Torbati, MB Beauvais, CH Vassetzky, Y Rosen, JM Devinoy, E AF Rijnkels, Monique Kabotyanski, Elena Montazer-Torbati, Mohamad B. Beauvais, C. Hue Vassetzky, Yegor Rosen, Jeffrey M. Devinoy, Eve TI The Epigenetic Landscape of Mammary Gland Development and Functional Differentiation SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Review DE Mammary gland; Epigenetic; Milk protein genes; Chromatin; Development ID BETA-CASEIN GENE; LUMINAL CELL-FATE; STEM-CELLS; TRANSCRIPTION FACTOR; CHROMATIN-STRUCTURE; EPITHELIAL-CELLS; DNA METHYLATION; BREAST-CANCER; EXTRACELLULAR-MATRIX; HISTONE ACETYLATION AB Most of the development and functional differentiation in the mammary gland occur after birth. Epigenetics is defined as the stable alterations in gene expression potential that arise during development and proliferation. Epigenetic changes are mediated at the biochemical level by the chromatin conformation initiated by DNA methylation, histone variants, post-translational modifications of histones, non-histone chromatin proteins, and non-coding RNAs. Epigenetics plays a key role in development. However, very little is known about its role in the developing mammary gland or how it might integrate the many signalling pathways involved in mammary gland development and function that have been discovered during the past few decades. An inverse relationship between marks of closed (DNA methylation) or open chromatin (DnaseI hypersensitivity, certain histone modifications) and milk protein gene expression has been documented. Recent studies have shown that during development and functional differentiation, both global and local chromatin changes occur. Locally, chromatin at distal regulatory elements and promoters of milk protein genes gains a more open conformation. Furthermore, changes occur both in looping between regulatory elements and attachment to nuclear matrix. These changes are induced by developmental signals and environmental conditions. Additionally, distinct epigenetic patterns have been identified in mammary gland stem and progenitor cell sub-populations. Together, these findings suggest that epigenetics plays a role in mammary development and function. With the new tools for epigenomics developed in recent years, we now can begin to establish a framework for the role of epigenetics in mammary gland development and disease. C1 [Rijnkels, Monique] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Kabotyanski, Elena; Rosen, Jeffrey M.] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Montazer-Torbati, Mohamad B.] Univ Birjand, Fac Agr, Dept Anim Sci, Birjand, Iran. [Beauvais, C. Hue; Devinoy, Eve] INRA, Genom & Physiol Lactat UR1196, F-78352 Jouy En Josas, France. [Vassetzky, Yegor] Univ Paris 11, CNRS, UMR 8126, Inst Cancerol Gustave Roussy, F-94805 Villejuif, France. RP Rijnkels, M (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. EM rijnkel@bcm.edu RI Vassetzky, Yegor/C-6447-2008 OI Vassetzky, Yegor/0000-0003-3101-7043 FU USDA/ARS [6250-51000-048-00]; NIH [1R21HD053762, 5R03HD56090, R37-CA16303-35]; Iranian Ministry of Science, Research and Technology [INRA-292, P00258] FX USDA/ARS 6250-51000-048-00, NIH 1R21HD053762, and NIH 5R03HD56090 to MR; NIH R37-CA16303-35 to JMR; Iranian Ministry of Science, Research and Technology to MBMT and INRA-292 and P00258 to ED NR 188 TC 45 Z9 47 U1 1 U2 8 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD MAR PY 2010 VL 15 IS 1 SI SI BP 85 EP 100 DI 10.1007/s10911-010-9170-4 PG 16 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 557FZ UT WOS:000274656300008 PM 20157770 ER PT J AU Feldlaufer, MF Domingue, MJ Chauhan, KR Aldrich, JR AF Feldlaufer, Mark F. Domingue, Michael J. Chauhan, Kamlesh R. Aldrich, Jeffrey R. TI 4-Oxo-Aldehydes From the Dorsal Abdominal Glands of the Bed Bug (Hemiptera: Cimicidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE bed bugs; Cimex lectularius; semiochemicals; dorsal abdominal glands ID CIMEX-LECTULARIUS L; HEPATITIS-B VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; ALARM PHEROMONE; DEFENSIVE SECRETIONS; CHEMICAL ECOLOGY; L HETEROPTERA; STINK BUGS; TRANSMISSION; CHEMISTRY AB Analyses of the dorsal abdominal glauds Of fourth- and fifth-instar nymphs of the bed bud, Cimex lectularius L. (Hemiptera: Cimicidae), indicated the predominant coustituents were (E)-2-hexenal and (E)-2-octenal, with lesser amounts of 4-oxo-(E)-2-hexenal and 4-oxo-(E)-2-octenal. The latter two compounds have not been reported previously as occurring in bed bugs. There were no differences in the chemical composition of the dorsal abdominal glands excised from exuviae left behind by either male or female adults, nor from glands excised from fourth-instar exuviae. Because the two oxo-aldehydes made tip at least 1.6% of the gland contents, further study of the functional role of these chemicals seem advisable. C1 [Feldlaufer, Mark F.; Domingue, Michael J.; Chauhan, Kamlesh R.; Aldrich, Jeffrey R.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Feldlaufer, MF (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. EM mark.feldlaufer@ars.usda.gov RI Domingue, Michael/G-1361-2011 NR 34 TC 21 Z9 22 U1 0 U2 27 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2010 VL 47 IS 2 BP 140 EP 143 DI 10.1603/ME09210 PG 4 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 566TW UT WOS:000275397100004 PM 20380293 ER PT J AU Pridgeon, JW Becnel, JJ Bernier, UR Clark, GG Linthicum, KJ AF Pridgeon, Julia W. Becnel, James J. Bernier, Ulrich R. Clark, Gary G. Linthicum, Kenneth J. TI Structure-Activity Relationships of 33 Carboxamides as Toxicants Against Female Aedes aegypti (Diptera: Culicidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE carboxamide; mosquito control; dengue; Aedes aegypti; insecticide ID YELLOW-FEVER VIRUS; IN-FIELD POPULATIONS; INSECTICIDE RESISTANCE; TRANSMISSION; MOSQUITOS; DENGUE; REPELLENTS; ALBOPICTUS; THAILAND; OUTBREAK AB Aede aegypti L. is the primary vector of dengue and yellow fever viruses, and use of aerosolized insecticides is one of the primary ways to control this medically important mosquito. However, few new insecticides have been developed for mosquito control in recent years. As it part of our effort to search for new insecticides to control mosquitoes, toxicities of 33 carboxamides were evaluated against female A. aegypti by topical application. This group included nine different categories of compounds, namely benzamides, phenyl-propenamides, propanamides, butanamides, butenamides, pentanamides, pentenamides, hexanamides, and hexenamides, that exhibited varying levels of toxicity against this mosquito species. The Most toxic compound tested was hexahydro-1-(1-oxohexyl)-1H-azepine, with a 24-h LD(50) value of 0.4 mu g per mosquito, whereas the most toxic compound at the LD(95) level was N-ethyl-2-methyl-N-phenyl-benzamide (1.82 mu g per mosquito). The least toxic compound was N,N-bis (2-methylpropyl)-3-phenyl-2-propenamide, with LD(50) and LD(95) values of 15.66 and 72.07 mu g per mosquito, respectively. Results front this initial study may prove useful in guiding further carboxamide modifications for the development of potential new insecticides. C1 [Pridgeon, Julia W.; Becnel, James J.; Bernier, Ulrich R.; Clark, Gary G.; Linthicum, Kenneth J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Pridgeon, JW (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM julia.pridgeon@ars.usda.gov NR 33 TC 1 Z9 1 U1 2 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2010 VL 47 IS 2 BP 172 EP 178 DI 10.1603/ME08265 PG 7 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 566TW UT WOS:000275397100008 PM 20380297 ER PT J AU Kramer, M Feldlaufer, MF Chauhan, KR AF Kramer, Matthew Feldlaufer, Mark F. Chauhan, Kamlesh R. TI Mosquito Biting Behavior: Statistical Power and Sources of Variation in Toxicity and Repellent Bioassays SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE mosquito bioassays; statistical power; variance components; biting pressure; Aedes aegypti ID DEET; INSECTICIDES; EFFICACY; SYSTEM AB Compounds thought to be effective against mosquitoes as either 'insecticides' oil repellents' have recently been shown to contain properties of both, or possess other behavior modifying actions. Prompted in part by these reports, we conducted posterior analyses of our data to examine some interrelated statistical issues inherent in our bioassay system. Using a modified K&D module, the responses of over 25,000 adult Aedes aegupti (L.) females exposed to either alphacypermethrin or DEET were compared with the responses of mosquitoes exposed to untreated controls for toxicity and biting (alphacypermethrin) or biting alone (DEET). Our analyses indicated that; (1) our bioassay system has more statistical power to determine a compound's toxicity than its repellent qualities, (2) day-to-day variability is large and needs to be accommodated in analyses; there are other, potentially even larger sources of variability (e.g., mosquito heterogeneity) which invalidate statistical tests that are based on the assumption of binomially or multinomially distributed data (e.g., chi(2) tests), and (3) unlike biting mosquitoes exposed to DEET, the proportions of biting mosquitoes exposed to alphacypermethrin are unrelated to the proportions of concurrently tested biting controls, even after adjusting for daily variation in toxicity. Thus, there is a clear behavioral indicator in this bioassay system that the 'repellency' of DEET (a presumed repellent) differs in a fundamental way from that of alphacypermethrin (a presumed toxicant), which may allow the differentiation between classes of compounds based on biting behavior alone. C1 [Kramer, Matthew; Feldlaufer, Mark F.; Chauhan, Kamlesh R.] USDA ARS, Biometr Consulting Serv, Beltsville, MD 20705 USA. RP Kramer, M (reprint author), USDA ARS, Biometr Consulting Serv, 10300 Baltimore Ave,Bldg 005,Room 130, Beltsville, MD 20705 USA. EM matt.kramer@ars.usda.gov RI Marion-Poll, Frederic/D-8882-2011 OI Marion-Poll, Frederic/0000-0001-6824-0180 NR 20 TC 5 Z9 5 U1 2 U2 3 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2010 VL 47 IS 2 BP 199 EP 204 DI 10.1603/ME09155 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 566TW UT WOS:000275397100011 PM 20380300 ER PT J AU Larson, RT Lorch, JM Pridgeon, JW Becnel, JJ Clark, GG Lan, Q AF Larson, Ryan T. Lorch, Jeffrey M. Pridgeon, Julia W. Becnel, James J. Clark, Gary G. Lan, Que TI The Biological Activity of alpha-Mangostin, a Larvicidal Botanic Mosquito Sterol Carrier Protein-2 Inhibitor SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE sterol carrier protein-2; larvicide; mangostin; detoxification enzymes; mosquitoes ID AEDES-AEGYPTI DIPTERA; YELLOW-FEVER MOSQUITO; INSECTICIDE RESISTANCE; EXPRESSION; XANTHONES; CULICIDAE; GENE; METABOLISM; ACID; MICE AB alpha-Mangostin derived from mangosteen was identified as a mosquito sterol carrier protein-2 inhibitor via high throughput insecticide screening. alpha-Mangostin was tested for its larvicidal activity against third instar larvae of six mosquito species, and the median lethal concentration values range from 0.84 to 2.90 ppm. The residual larvicidal activity of a-mangostin was examined under semifield conditions. The results indicated that a-mangostin was photolytic with a half-life of 53 min in water under full sunlight exposure. The effect of a-mangostin on activities of major detoxification enzymes such as P450, glutathione S-transferase, and esterase was investigated. The results showed that a-mangostin significantly elevated activities of P450 and glutathione S-transferase in larvae, whereas it suppressed esterase activity. Toxicity Of alpha-mangostin against young rats was studied, and there was no detectable adverse effect at dosages as high as 80 mg/kg. This is the first multifaceted study of the biological activity of alpha-mangostin in mosquitoes. The results suggest that alpha-mangostin may be I lead compound for the development of a new organically based mosquito larvicide. C1 [Lan, Que] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Larson, Ryan T.; Lan, Que] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Lorch, Jeffrey M.] Univ Wisconsin, Mol & Environm Toxicol Ctr, Madison, WI 53706 USA. [Pridgeon, Julia W.; Becnel, James J.; Clark, Gary G.] USDA ARS, Ctr Med & Vet Entomol, Gainesville, FL 32608 USA. RP Lan, Q (reprint author), Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM qlan@entomology.wise.edu OI Lorch, Jeffrey/0000-0003-2239-1252 FU U.S. Navy Entomology Health Services; University of Wisconsin College of Agriculture; U.S. Armed Forces Pest Management Board of the U.S. Department of Defense [W9113M-05-1-0006]; National Institutes of Health [5R01AI067422] FX We thank Drs. Bruce Christensen and Susan Paskewitz for providing the mosquito eggs, and David H. Dyer for his technical support. This work was Supported by the U.S. Navy Entomology Health Services Collegiate Program (HSCP) (to R.T.L.); the University of Wisconsin College of Agriculture; Grant W9113M-05-1-0006 from the Deployed War Fighter Protection Research Program, which was administered by the U.S. Armed Forces Pest Management Board of the U.S. Department of Defense; and National Institutes of Health Research Grant 5R01AI067422 (to QL.). NR 34 TC 19 Z9 22 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2010 VL 47 IS 2 BP 249 EP 257 DI 10.1603/ME09160 PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 566TW UT WOS:000275397100018 PM 20380307 ER PT J AU Becker, ME Reeves, WK Dejean, SK Emery, MP Ostlund, EN Foil, LD AF Becker, M. E. Reeves, W. K. Dejean, S. K. Emery, M. P. Ostlund, E. N. Foil, L. D. TI Detection of Bluetongue Virus RNA in Field-Collected Culicoides spp. (Diptera: Ceratopogonidae) Following the Discovery of Bluetongue Virus Serotype 1 in White-Tailed Deer and Cattle in Louisiana SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Ceratopogonidae; Culicoides; bluetongue virus ID HEMORRHAGIC-DISEASE; UNITED-STATES; VARIIPENNIS; PCR AB In November 2004, bluetongue virus (family Reoviridae, genus Orbivirus, BTV) serotype 1 (BTV-1) was detected for the first time in the United States from a hunter-killed deer in St. Mary Parish, LA. In 2005, sera surveys were conducted on three cattle farms near the area where the deer was found, and BTV-1-seropositive cattle were found oil two of the three farms; in 2006, sera surveys from the cattle on the three farms did not detect any BTV-1-positive animals. The purpose of this study was to survey ceratopogonid populations at the three farms and test field-collected specimens for the presence of BTV and epizootic hemorrhagic disease virus (family Reoviridae, genus Orbivirus, EHDV). Miniature CDC light traps and New Jersey traps were used to capture ceratopogonids oil the three farms from January 2006 through November 2007. In total, 3,319 ceratopogonids were captured, including 1,790 specimens of 10 different species of Culicoides. IR-RT-polymerase chain reaction (PCR) was performed to screen for BTV and EHDV in 264 pools representing 2,309 specimens collected at the farms. All positive samples were sequenced for serotype determination. Five pools of 275 (1.8%) were positive for BTV. Pools of four species of Culicoides were found to be positive: Culicoides crepuscularis (Malloch), Culicoides debilipalpis Lutz (two pools), Culicoides haematopotus Malloch, and Culicoides furens (Poey). The amplicons of the positive specimens were sequenced and found to be identical to both BTV-17 and BTV-13. During our study, no BTV-1 transmission was detected in cattle, and no BTV-1 was detected in specimens of ceratopogonids. C1 [Becker, M. E.; Foil, L. D.] Louisiana State Univ, Ctr Agr, Dept Entomol, Agr Expt Stn, Baton Rouge, LA 70803 USA. [Reeves, W. K.] Coll Agr, USDA ARS, Arthropod Borne Anim Dis Res Lab, Laramie, WY 82072 USA. [Dejean, S. K.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Baton Rouge, LA 70802 USA. [Emery, M. P.; Ostlund, E. N.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA. RP Becker, ME (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, Agr Expt Stn, 402 Life Sci, Baton Rouge, LA 70803 USA. EM mbecker@agcenter.lsu.edu FU USDA-CSREES FX We thank Cecilia Kato, Andy Fabian, Cassidy Lejeune, and the Louisiana Department of Wildlife and Fisheries for contributions to this Project. We also thank Wayne Cantrell, Joe Trahan, and Kevin Brightwell for allowing LIS to trap Oil their farms. We thank Wayne Kramer and Kevin Macaluso for review of and suggestions for the manuscript. We also thank Fred Enright, LSU Agricultural Center, and David Stallknecht, University of Georgia, for valuable information regarding this manuscript. This article was published with the approval of the Director of Louisiana Agricultural Experiment Station as manuscript 2009-234-3652. This work was partially funded by a USDA-CSREES Critical Issues in New and Emerging Animal Diseases grant. NR 19 TC 7 Z9 7 U1 0 U2 2 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2010 VL 47 IS 2 BP 269 EP 273 DI 10.1603/ME09211 PG 5 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 566TW UT WOS:000275397100020 PM 20380309 ER PT J AU Coates, BS Sumerford, DV Hellmich, RL Lewis, LC AF Coates, Brad S. Sumerford, Douglas V. Hellmich, Richard L. Lewis, Leslie C. TI A Helitron-Like Transposon Superfamily from Lepidoptera Disrupts (GAAA)(n) Microsatellites and is Responsible for Flanking Sequence Similarity within a Microsatellite Family SO JOURNAL OF MOLECULAR EVOLUTION LA English DT Article DE Microsatellite family; Transposable element; Mobile element ID ROLLING-CIRCLE TRANSPOSONS; INVERTED-REPEAT ELEMENTS; YELLOW-FEVER MOSQUITO; SILKWORM BOMBYX-MORI; REPETITIVE ELEMENTS; GENE-EXPRESSION; EVOLUTIONARY DYNAMICS; INTIMATE ASSOCIATION; ARABIDOPSIS-THALIANA; GENOME SEQUENCE AB Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A + T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA)(n) microsatellite loci, and was named the microsatellite-associated interspersed nuclear element (MINE-1). Non-autonomous MINE-1 superfamily members also were identified downstream of (GAAA)(n) microsatellites within Bombyx mori and Pectinophora gossypiella genomes. Of 316 (GAAA)(n) microsatellites from the B. mori whole genome sequence, 201 (63.6%) have associated autonomous or non-autonomous MINE-1 elements. Autonomous B. mori MINE-1s a encode a helicase and endonuclease domain RepHel-like protein (BMHELp1) indicating their classification as Helitron-like transposons and were renamed Helitron1_BM. Transposition of MINE-1 members in Lepidoptera has resulted in the disruption of (GAAA)(n) microsatellite loci, has impacted the application of microsatellite-based genetic markers, and suggests genome sequence that flanks TT/AA dinucleotides may be required for target site recognition by RepHel endonuclease domains. C1 [Coates, Brad S.; Sumerford, Douglas V.; Hellmich, Richard L.; Lewis, Leslie C.] Iowa State Univ, USDA ARS, Corn Insect & Crop Genet Res Unit, Ames, IA 50011 USA. [Sumerford, Douglas V.; Hellmich, Richard L.; Lewis, Leslie C.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. RP Coates, BS (reprint author), Iowa State Univ, USDA ARS, Corn Insect & Crop Genet Res Unit, 113 Genet Lab, Ames, IA 50011 USA. EM brad.coates@ars.usda.gov FU United States Department of Agriculture Agriculture Research Service; Iowa Agriculture and Home Economics Experiment Station, Ames; [3543] FX This research is a joint contribution from the United States Department of Agriculture Agriculture Research Service and the Iowa Agriculture and Home Economics Experiment Station, Ames (project 3543). Mention of proprietary products does not constitute an endorsement or a recommendation by USDA, or Iowa State University for its use. NR 74 TC 21 Z9 21 U1 2 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0022-2844 J9 J MOL EVOL JI J. Mol. Evol. PD MAR PY 2010 VL 70 IS 3 BP 275 EP 288 DI 10.1007/s00239-010-9330-6 PG 14 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 575NB UT WOS:000276072800006 PM 20217059 ER PT J AU Schmidt, LE Deyrup, ST Baltrusaitis, J Swenson, DC Wicklow, DT Gloer, JB AF Schmidt, Lori E. Deyrup, Stephen T. Baltrusaitis, Jonas Swenson, Dale C. Wicklow, Donald T. Gloer, James B. TI Hymenopsins A and B and a Macrophorin Analogue from a Fungicolous Hymenopsis sp. SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID GAUSSIAN-BASIS SETS; FRUIT ROT; METABOLITES; CONSTITUENTS; ENERGIES; FUNGUS; APPLE AB Hymenopsin A (1), hymenopsin B (2), and a new macrophorin analogue, 2',3'-epoxy-13-hydroxy-4'-oxomacrophorin A (3), have been isolated from a fungicolous isolate of Hymenopsis sp. (MYC-1703; NRRL 37638). The structures and relative configurations of these compounds were assigned on the basis of 2D NMR and MS data, and the identity of 1 was confirmed by X-ray crystallographic analysis. The absolute configuration of 2 was proposed on the basis of CD analysis using both empirical and computational methods. Compounds 2 and 3 showed antibacterial activity against Staphylococcus aureus and Bacillus subtilis. Compound 3 was also active against Aspergillus flavus and Fusurium verticillioides. C1 [Schmidt, Lori E.; Deyrup, Stephen T.; Baltrusaitis, Jonas; Swenson, Dale C.; Gloer, James B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, Donald T.] ARS, Mycotoxin Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Gloer, JB (reprint author), Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. EM james-gloer@uiowa.edu RI Baltrusaitis, Jonas/F-9367-2013; OI Deyrup, Stephen/0000-0002-1851-5593 FU NIGMS NIH HHS [GM 60600, R01 GM060600, R01 GM060600-07] NR 27 TC 9 Z9 9 U1 1 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD MAR PY 2010 VL 73 IS 3 SI SI BP 404 EP 408 DI 10.1021/np900613d PG 5 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA 573CC UT WOS:000275885000020 PM 19928955 ER PT J AU Kokalis-Burelle, N Rosskopf, EN Albano, JP Holzinger, J AF Kokalis-Burelle, Nancy Rosskopf, Erin N. Albano, Joseph P. Holzinger, John TI Effects of Midas (R) on Nematodes in Commercial Floriculture Production in Florida SO JOURNAL OF NEMATOLOGY LA English DT Article DE Celosia argentea; floriculture; iodomethane; methyl bromide; root-knot nematodes; management; Meloidogyne arenaria; Midas (R) ID METHYL-BROMIDE; IDENTIFICATION; PHENOTYPES AB Cut flower producers currently have limited options for nematode control. Four field trials were conducted in 2006 and 2007 to evaluate Midas (R) (iodomethane:chloropicrin 50:50) for control of root-knot nematodes (Meloidogyne arenaria) on Celosia argentea var. cristata in a commercial floriculture production field in southeastern Florida. Midas (224 kg/ha) was compared to methyl bromide:chloropicrin (98:2, 224 kg/ha), and an untreated control. Treatments wore evaluated for effects on Meloidogyne arenaria J2 and free-living nematodes in soil through each season, and roots at the end of each season. Plant growth and root disease were also assessed. Population levels of nematodes isolated from soil were highly variable in all trials early in the season, and generally rebounded by harvest, sometimes to higher levels in fumigant treatments than in the untreated control. Although population levels of nematodes in soil were not significantly reduced during the growing season, nematodes in roots and galling at the end of the season were consistently reduced with both methyl bromide and Midas compared to the untreated control. Symptoms of phytotoxicity were observed in Midas treatments during the first year and were attributed to Fe toxicity. Fertilization was adjusted during the second year to investigate potential fumigant/fertilizer interactions. Interactions occurred at the end of the fourth trial between methyl bromide and fertilizers with respect to root-knot nematode J2 isolated from roots and galling. Fewer J2 were isolated from roots treated with a higher level of Fe (3.05%) in the form of Fe sucrate, and galling was reduced in methyl bromide treated plots treated with this fertilizer compared to Fe EDTA. Reduced galling was also seen with Midas in Fe sucrate fertilized plots compared to Fe EDTA. This research demonstrates the difficulty of reducing high root-knot nematode population levels in soil in subtropical conditions in production fields that have been repeatedly fumigated. Although soil population density may remain stable, root population density and disease can be reduced. C1 [Kokalis-Burelle, Nancy; Rosskopf, Erin N.; Albano, Joseph P.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Holzinger, John] Holzinger Flowers Inc, Palm City, FL USA. RP Kokalis-Burelle, N (reprint author), ARS, USDA, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM nancy.burelle@ars.usda.gov NR 15 TC 6 Z9 6 U1 0 U2 4 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2010 VL 42 IS 1 BP 17 EP 21 PG 5 WC Zoology SC Zoology GA 682FU UT WOS:000284387600003 PM 22736832 ER PT J AU Carta, LK Bauchan, G Hsu, CY Yuceer, C AF Carta, L. K. Bauchan, G. Hsu, C. -Y. Yuceer, C. TI Description of Parasitorhabditis frontali n. sp. (Nemata: Rhabditida) from Dendroctonus frontalis Zimmermann (Coleoptera: Scolytidae) SO JOURNAL OF NEMATOLOGY LA English DT Article DE morphology; nematode; Parasitorhabditis frontali n. sp.; SEM; southern pine beetle; taxonomy; trophic interaction ID FUNGUS AB A new Parasitorhabditis species with males and females was discovered from the southern pine beetle Dendroctonus frontalis and its galleries in loblolly pine. Pinus taeda, growing in Mississippi. Females of the new species have a cupola-shaped tail with a small spike; males possess a 2 + (3+2) + 3 ray pattern on the tail fan with ray 10 reaching the margin, and a distinctive stomatal tooth. Parasitorhabditis frontali n. sp. has some similarities to P. hylurgi Massey, 1974 from Hylurgops pinifex in New York, USA, P. terebranus Massey, 1974 from D. terebrans (Olivier, 1795) in Texas USA, P. ligniperdae Fuchs, 1915 from Hylergops ligniperda (Fabricius, 1787) and P dendroctoni Ruhm, 1956 from D. micans (Kugelann, 1794) in Europe, P alert Fuchs, 1915 isolated from the beetle Hylastes ater (Paykull, 1800) in Germany and P. malii Devdariani and Kakulia, 1970 from Scolytus mali (Bechstein, 1805) within the republic of Georgia. Morphometrics for 44 species of Parasitorhabditis are provided to update older keys. Parasitorhabditis frontali n. sp. was initially grown on Malt Extract (ME) agar with its own microbial contaminants that included a bacterium and fungus. The nematode also grew and reproduced after slices of ME agar with nematodes and microbial contaminants were transferred to water agar. It was killed by E. coli on NGM agar plates commonly used to raise other Rhabditida. Drawings of diagnostic anatomy and low-temperature SEM images of bodies, heads, and tails are provided for cultured specimens from pine beetle frass. C1 [Carta, L. K.] ARS, USDA, Nematol Lab, Beltsville, MD 20705 USA. [Bauchan, G.] ARS, USDA, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Hsu, C. -Y.; Yuceer, C.] Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA. RP Carta, LK (reprint author), ARS, USDA, Nematol Lab, Beltsville, MD 20705 USA. EM lynn.carta@ars.usda.gov NR 22 TC 1 Z9 2 U1 1 U2 6 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2010 VL 42 IS 1 BP 46 EP 54 PG 9 WC Zoology SC Zoology GA 682FU UT WOS:000284387600007 PM 22736836 ER PT J AU Hall, LM Kimlin, MG Aronov, PA Hammock, BD Slusser, JR Woodhouse, LR Stephensen, CB AF Hall, Laura M. Kimlin, Michael G. Aronov, Pavel A. Hammock, Bruce D. Slusser, James R. Woodhouse, Leslie R. Stephensen, Charles B. TI Vitamin D Intake Needed to Maintain Target Serum 25-Hydroxyvitamin D Concentrations in Participants with Low Sun Exposure and Dark Skin Pigmentation Is Substantially Higher Than Current Recommendations SO JOURNAL OF NUTRITION LA English DT Article ID ULTRAVIOLET-RADIATION EXPOSURE; SOLAR UV-RADIATION; CUTANEOUS SYNTHESIS; US POPULATION; D METABOLITES; PHOTOSYNTHESIS; LATITUDE; PREVITAMIN-D3; ASSOCIATION; PERSPECTIVE AB Cutaneous cholecalciferol synthesis has not been considered in making recommendations for vitamin D intake. Our objective was to model the effects of sun exposure, vitamin D intake, and skin reflectance (pigmentation) on serum 25-hydroxyvitamin D (25[OH]D) in young adults with a wide range of skin reflectance and sun exposure. Four cohorts of participants (n = 72 total) were studied for 7-8 wk in the fall, winter, spring, and summer in Davis, CA [38.5 degrees N, 121.7 degrees W, Elev. 49 ft (15 m)]. Skin reflectance was measured using a spectrophotometer, vitamin D intake using food records, and sun exposure using polysulfone dosimeter badges. A multiple regression model (R-2 = 0.55; P < 0.0001) was developed and used to predict the serum 25(OH)D concentration for participants with low [median for African ancestry (AA)] and high [median for European ancestry (EA)] skin reflectance and with low (20th percentile, similar to 20 min/d, similar to 18% body surface area (BSA) exposed) and high (80th percentile, similar to 90 min/d, similar to 35% BSA exposed) sun exposure, assuming an intake of 200 IU/d (5 ug/d). Predicted serum 25(OH)D concentrations for AA individuals with low and high sun exposure in the winter were 24 and 42 nmol/L and in the summer were 40 and 60 nmol/L. Corresponding values for EA individuals were 35 and 60 nmol/L in the winter and in the summer were 58 and 85 nmol/L. To achieve 25(OH)D >= 75 nmol/L, we estimate that EA individuals with high sun exposure need 1300 IU/d vitamin D intake in the winter and AA individuals with low sun exposure need 2100-3100 IU/d year-round. J. Nutr. 140: 542-550, 2010. C1 [Hall, Laura M.; Stephensen, Charles B.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Aronov, Pavel A.; Hammock, Bruce D.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Aronov, Pavel A.; Hammock, Bruce D.] Univ Calif Davis, Ctr Canc, Davis, CA 95616 USA. [Hall, Laura M.; Woodhouse, Leslie R.; Stephensen, Charles B.] USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Kimlin, Michael G.] Queensland Univ Technol, AusSun Res Lab, Inst Biomed Innovat, Kelvin Grove, Qld 4059, Australia. [Slusser, James R.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Hall, LM (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM lauhall@calpoly.edu OI Kimlin, Michael/0000-0002-9536-8646 FU NIH [P60MD0222]; USDA [5306-51530-006-00D]; Bristol-Myers Squibb Foundation, Inc.; Gustavus and Louise Pfeiffer Research Foundation; National Institute of Environmental Health Sciences (NIEHS) Center for Children's Environmental Health and Disease Prevention [P01 ES011269]; U.S. Environmental Protection Agency (EPA) Center for Children's Environmental Health and Disease Prevention [R833292, R829388]; NIEHS/EPA Children's Center; California Dairy Research Foundation [07 HAB-01-NH]; NIEHS [T32 ES007059, P42 ES004699]; Autism Speaks grant [4933]; Queensland Cancer Council FX Supported by NIH grant P60MD0222 (to L.M.H. and C.B.S.), USDA CRIS project no. 5306-51530-006-00D, a Bristol-Myers Squibb Foundation, Inc. Freedom to Discover Grant, and a grant from the Gustavus and Louise Pfeiffer Research Foundation. P.A.A. and B.D.H. are supported by the National Institute of Environmental Health Sciences (NIEHS) Center for Children's Environmental Health and Disease Prevention P01 ES011269, the U.S. Environmental Protection Agency (EPA) Center for Children's Environmental Health and Disease Prevention R833292 and R829388, the NIEHS/EPA Children's Center, the California Dairy Research Foundation grant 07 HAB-01-NH, the NIEHS Advanced Training in Environmental Toxicology grant T32 ES007059, the Autism Speaks grant 4933 (analytical work), and the NIEHS Superfund grant P42 ES004699 (analytical work). M.G.K. is supported through a Queensland Cancer Council Senior Research Fellowship. NR 49 TC 42 Z9 42 U1 0 U2 8 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 2010 VL 140 IS 3 BP 542 EP 550 DI 10.3945/jn.109.115253 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 559FJ UT WOS:000274807800016 PM 20053937 ER PT J AU Durazo-Arvizu, RA Dawson-Hughes, B Sempos, CT Yetley, EA Looker, AC Cao, GC Harris, SS Burt, VL Carriquiry, AL Picciano, MF AF Durazo-Arvizu, Ramon A. Dawson-Hughes, Bess Sempos, Christopher T. Yetley, Elizabeth A. Looker, Anne C. Cao, Guichan Harris, Susan S. Burt, Vicki L. Carriquiry, Alicia L. Picciano, Mary Frances TI Three-Phase Model Harmonizes Estimates of the Maximal Suppression of Parathyroid Hormone by 25-Hydroxyvitamin D in Persons 65 Years of Age and Older SO JOURNAL OF NUTRITION LA English DT Article ID LOCALLY WEIGHTED REGRESSION; VITAMIN-D STATUS; HYPOVITAMINOSIS D; SERUM; WOMEN AB The concentration or threshold of 25-hydroxyvitamin D [25(OH)D] needed to maximally suppress intact serum parathyroid hormone (iPTH) has been suggested as a measure of optimal vitamin D status. Depending upon the definition of maximal suppression of iPTH and the 2-phase regression approach used, 2 distinct clusters for a single 25(OH)D threshold have been reported: 16-20 ng/mL (40-50 nmol/L) and 30-32 ng/mL (75-80 nmol/L). To rationalize the apparently disparate published results, we compared thresholds from several regression models including a 3-phase one to estimate simultaneously 2 thresholds before and after adjusting for possible confounding for age, BMI, glomerular filtration rate, dietary calcium, and season (April-September vs. October-March) within a single data set, i.e. data from the Tufts University Sites Testing Osteoporosis Prevention/Intervention Treatment study, consisting of 181 men and 206 women (total n = 387) ages 65-87 y. Plasma 25(OH)D and serum iPTH concentrations were (mean +/- SD) 22.1 +/- 7.44 ng/mL (55.25 +/- 18.6 nmol/L) and 36.6 +/- 16.03 pg/mL (3.88 +/- 1.7 pmol/L), respectively. The 3-phase model identified 2 thresholds of 12 ng/mL (30 nmol/L) and 28 ng/mL (70 nmol/L); similar results were found from the 2-phase models evaluated, i.e. 13-20 and 27-30 ng/mL (32.5-50 and 67.5-75 nmol/L) and with previous results. Adjusting for confounding did not change the results substantially. Accordingly, the 3-phase model appears to be superior to the 2-phase approach, because it simultaneously estimates the 2 threshold clusters found from the 2-phase approaches along with estimating confidence limits. If replicated, it may be of both clinical and public health importance. J. Nutr. 140: 595-599, 2010. C1 [Sempos, Christopher T.; Yetley, Elizabeth A.; Picciano, Mary Frances] Natl Inst Hlth, Off Dietary Supplements, Bethesda, MD 20816 USA. [Durazo-Arvizu, Ramon A.; Cao, Guichan] Loyola Univ Chicago, Dept Prevent Med & Epidemiol, Stritch Sch Med, Maywood, IL 60153 USA. [Dawson-Hughes, Bess; Harris, Susan S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Bone Metab Lab, Boston, MA 02111 USA. [Looker, Anne C.; Burt, Vicki L.] Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Hyattsville, MD 20782 USA. [Carriquiry, Alicia L.] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. RP Sempos, CT (reprint author), Natl Inst Hlth, Off Dietary Supplements, Bethesda, MD 20816 USA. EM semposch@mail.nih.gov FU National Institute on Aging, NIH, Bethesda, MD [AG10353]; Office of Dietary Supplements [5R37 HL045508-17] FX Supported by a grant from the National Institute on Aging, NIH, Bethesda, MD, grant number AG10353, and by an Office of Dietary Supplements administrative supplement to NIH grant number 5R37 HL045508-17. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the CDC, the NIH, or the US Department of Health and Human Services. NR 23 TC 31 Z9 35 U1 0 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAR PY 2010 VL 140 IS 3 BP 595 EP 599 DI 10.3945/jn.109.116681 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 559FJ UT WOS:000274807800023 PM 20089790 ER PT J AU Eneroth, H el Arifeen, S Persson, LA Lonnerdal, B Hossain, MB Stephensen, CB Ekstrom, EC AF Eneroth, Hanna el Arifeen, Shams Persson, Lars-Ake Lonnerdal, Bo Hossain, Mohammad Bakhtiar Stephensen, Charles B. Ekstrom, Eva-Charlotte TI Maternal Multiple Micronutrient Supplementation Has Limited Impact on Micronutrient Status of Bangladeshi Infants Compared with Standard Iron and Folic Acid Supplementation SO JOURNAL OF NUTRITION LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; VITAMIN-A SUPPLEMENTATION; BETA-CAROTENE SUPPLEMENTATION; SERUM ZINC CONCENTRATIONS; PREGNANT-WOMEN; DOUBLE-BLIND; COBALAMIN STATUS; CHILD-MORTALITY; BIRTH-WEIGHT; RURAL NEPAL AB Knowledge about the impact of maternal food and micronutrient supplementation on infant micronutrient status is limited. We examined the effect of maternal food and micronutrient supplementation on infant micronutrient status in the Maternal and Infant Nutrition Interventions in Matlab Trial. Pregnant women (n = 4436) were randomized to Early or Usual promotion of enrollment in a food supplementation program. In addition, they were randomly allocated to 1 of the following 3 types of daily micronutrient supplements provided from wk 14 of gestation to 3 mo postpartum: 1) folic acid and 30 mg iron (Fe30Fol); 2) folic acid and 60 mg iron; or 3) a multiple micronutrient including folic acid and 30 mg iron (MMS). At 6 mo, infant blood samples (n = 1066) were collected and analyzed for hemoglobin and plasma ferritin, zinc, retinol, vitamin B-12, and folate. The vitamin B-12 concentration differed between the micronutrient supplementation groups (P = 0.049). The prevalence of vitamin B-12 deficiency was lower in the MMS group (26.1 %) than in the Fe30Fol group (36.5%) (P = 0.003). The prevalence of zinc deficiency was lower in the Usual food supplementation group (54.1 %) than in the Early group (60.2%) (P = 0.046). There were no other differential effects according to food or micronutrient supplementation groups. We conclude that maternal multiple micronutrient supplementation may have a beneficial effect on vitamin B-12 status in infancy. J. Nutr. 140: 618-624, 2010. C1 [Eneroth, Hanna; Persson, Lars-Ake; Ekstrom, Eva-Charlotte] Uppsala Univ, Dept Womens & Childrens Hlth, SE-75185 Uppsala, Sweden. [el Arifeen, Shams] Int Ctr Diarrhoeal Dis Res, Child Hlth Unit, Dhaka 1000, Bangladesh. [Hossain, Mohammad Bakhtiar] Int Ctr Diarrhoeal Dis Res, Nutr Biochem Lab, Dhaka 1000, Bangladesh. [Lonnerdal, Bo; Stephensen, Charles B.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Stephensen, Charles B.] Univ Calif Davis, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Eneroth, H (reprint author), Uppsala Univ, Dept Womens & Childrens Hlth, SE-75185 Uppsala, Sweden. EM hanna.eneroth@kbh.uu.se FU United Nations Children's Fund; Swedish International Development Cooperation Agency; UK Medical Research Council; Swedish Research Council; Department for International Development; International Centre for Diarrhoeal Disease Research, Bangladesh; Global Health Research Fund-Japan; Child Health and Nutrition Research Initiative; Uppsala University; United States Agency for International Development FX Supported by the United Nations Children's Fund, Swedish International Development Cooperation Agency, UK Medical Research Council, Swedish Research Council, Department for International Development, International Centre for Diarrhoeal Disease Research, Bangladesh, Global Health Research Fund-Japan, Child Health and Nutrition Research Initiative, Uppsala University, and United States Agency for International Development (Maternal and Infant Nutrition Interventions in Matlab Trial). NR 43 TC 17 Z9 18 U1 1 U2 9 PU AMER SOC NUTRITIONAL SCIENCE PI BETHESDA PA 9650 ROCKVILLE PIKE, RM L-2407A, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 2010 VL 140 IS 3 BP 618 EP 624 DI 10.3945/jn.109.111740 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 559FJ UT WOS:000274807800027 PM 20053938 ER PT J AU Colby, SE Johnson, L Scheett, A Hoverson, B AF Colby, Sarah E. Johnson, LuAnn Scheett, Angela Hoverson, Bonita TI Nutrition Marketing on Food Labels SO JOURNAL OF NUTRITION EDUCATION AND BEHAVIOR LA English DT Article DE marketing; food labels; dietary fat; sodium; sugar; children ID HEALTH CLAIMS; OBESITY EPIDEMIC; PACKAGE SURVEY; CONSUMER; INFORMATION; PREVENTION; AMERICANS; INDUSTRY AB Objective: This research sought to determine how often nutrition marketing is used on labels of foods that are high in saturated fat, sodium, and/or sugar. Design and Setting: All items packaged with food labels (N = 56,900) in all 6 grocery stores in Grand Forks, ND were surveyed. Main Outcome Measure(s): Marketing strategy, nutrient label information, if the product was fruit/or milk based, and target age. Analysis: Frequency distributions were computed. Results: Forty-nine percent of all products contained nutrition marketing and of those, 48% had both nutrition marketing and were high in saturated fat, sodium and/or sugar (11%, 17%, and 31% respectively). Seventy-one percent of products marketed to children had nutrition marketing. Of those, 59% were high in saturated fat, sodium and/or sugar content, with more than half being high in sugar. The most commonly used nutrition marketing statements were "good source of calcium", "reduced/low/fat free", and "food company's health symbol". Conclusions and Implications: Nutrition marketing is commonly used on products high in saturated fat, sodium and/or sugar and is more often used on products marketed toward children than products marketed toward adults. Current food industry symbols may not be helping consumers select foods low in saturated fat, sodium or sugar. C1 [Colby, Sarah E.; Johnson, LuAnn; Scheett, Angela; Hoverson, Bonita] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. RP Colby, SE (reprint author), E Carolina Univ, Coll Human Ecol, Dept Nutr & Dietet, Rivers 333, Greenville, NC 27858 USA. EM colbys@ecu.edu FU Forks Human Nutrition Research Center FX This project was funded by the Grand Forks Human Nutrition Research Center. NR 34 TC 49 Z9 49 U1 1 U2 27 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1499-4046 J9 J NUTR EDUC BEHAV JI J. Nutr. Educ. Behav. PD MAR-APR PY 2010 VL 42 IS 2 BP 92 EP 98 DI 10.1016/j.jneb.2008.11.002 PG 7 WC Education, Scientific Disciplines; Nutrition & Dietetics SC Education & Educational Research; Nutrition & Dietetics GA 604PH UT WOS:000278290500005 PM 20096635 ER PT J AU Zoellner, J Connell, C Powers, A Avis-Williams, A Yadrick, K Bogle, ML AF Zoellner, Jamie Connell, Carol Powers, Alicia Avis-Williams, Amanda Yadrick, Kathy Bogle, Margaret L. TI Does a Six-Month Pedometer Intervention Improve Physical Activity and Health Among Vulnerable African Americans? A Feasibility Study SO JOURNAL OF PHYSICAL ACTIVITY & HEALTH LA English DT Article DE pedometry; health promotion; community-based research; intervention study; special needs population ID CULTURAL ACTIVITY PARTICIPATION; AMBULATORY ACTIVITY; MISSISSIPPI DELTA; BLOOD-PRESSURE; WOMEN; RESIDENTS; STEPS/DAY; PATTERNS; PROGRAM; ADULTS AB Background: Race/ethnic-specific physical activity patterns and biological responses to physical activity is one of the most understudied, yet critical aspects related to the development and adoption of physical activity recommendations. Methods: In this 6-month community walking intervention targeting African Americans, participants wore a pedometer and maintained a pedometer diary for the study duration. Outcome measures included height, weight, percent body fat, waist circumference, blood pressure, lipids and glucose. ANOVA, Pearson Correlations, and Kruskal-Wallis tests were used to examine changes in steps/day over each month of the intervention and explore associations among pedometer-determined physical activity and anthropometric/biological change scores from month 1 to 6. Results: The 83 participants were primarily African American (98%) women (94%). There was a significant increase in the average step/day beginning with 6665 (SD =3,396) during month I and increasing to 9232 (SD = 3670) steps/day during month 6 (F = 4.5, P < .0001). Associations among step counts and anthropometric/biological change scores were not significant. Conclusions: While this intervention resulted in significant increases in steps/day; it exemplifies that physical activity standards may be unachievable for some vulnerable, minority communities. Methodological considerations for exploring associations between changes in pedometer-determined step counts and anthropometric/biological outcomes are emphasized through this study. C1 [Zoellner, Jamie; Connell, Carol; Avis-Williams, Amanda; Yadrick, Kathy] Univ So Mississippi, Dept Nutr & Food Syst, Hattiesburg, MS 39406 USA. [Powers, Alicia] Furman Univ, Dept Hlth & Exercise Sci, Greenville, SC 29613 USA. [Bogle, Margaret L.] ARS, USDA, Little Rock, AR USA. RP Zoellner, J (reprint author), Virginia Polytech Inst & State Univ, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. RI Loureiro, Nuno/I-6400-2012 OI Loureiro, Nuno/0000-0002-1166-3219 NR 33 TC 19 Z9 19 U1 2 U2 5 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1543-3080 J9 J PHYS ACT HEALTH JI J. Phys. Act. Health PD MAR PY 2010 VL 7 IS 2 BP 224 EP 231 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 636LT UT WOS:000280737900010 PM 20484761 ER PT J AU Tajvidi, M Motie, N Rassam, G Falk, RH Felton, C AF Tajvidi, Mehdi Motie, Nazanin Rassam, Ghonche Falk, Robert H. Felton, Colin TI Mechanical Performance of Hemp Fiber Polypropylene Composites at Different Operating Temperatures SO JOURNAL OF REINFORCED PLASTICS AND COMPOSITES LA English DT Article DE composites; mechanical properties; temperature; hemp fiber; polypropylene AB In order to quantify the effect of temperature on the mechanical properties of hemp fiber polypropylene composites, formulations containing 25% and 40% (by weight) hemp fiber were produced and tested at three representative temperatures of 256, 296, and 336 K. Flexural, tensile, and impact tests, as well as dynamic mechanical analysis, were performed and the reduction in mechanical properties were evaluated. Impact resistance was independent of temperature, whereas flexural and tensile properties were strongly affected. The highest reductions were observed in stiffness (modulus) values and flexural properties were reduced to a higher degree. The reductions in mechanical properties were well explained by a simple quadratic curve-fitting procedure applied to experimental data. Dynamic mechanical analysis revealed no change in glass transition temperature when the fiber content was increased but the composite material had better temperature resistance at higher fiber content. The results of the present study will be helpful in determining the end-use application of these composite materials. C1 [Tajvidi, Mehdi; Motie, Nazanin] Univ Tehran, Dept Wood & Paper Sci & Technol, Fac Nat Resources, Karaj, Iran. [Rassam, Ghonche] Shaheed Rajaee Teacher Training Univ, Dept Wood Sci & Technol, Fac Civil, Tehran, Iran. [Falk, Robert H.] USDA, Forest Prod Lab, Madison, WI 53705 USA. RP Tajvidi, M (reprint author), Univ Tehran, Dept Wood & Paper Sci & Technol, Fac Nat Resources, Karaj, Iran. EM mtajvidi@ut.ac.ir RI Tajvidi, Mehdi/D-6959-2012 NR 15 TC 17 Z9 17 U1 0 U2 5 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0731-6844 J9 J REINF PLAST COMP JI J. Reinf. Plast. Compos. PD MAR PY 2010 VL 29 IS 5 BP 664 EP 674 DI 10.1177/0731684408100266 PG 11 WC Materials Science, Composites; Polymer Science SC Materials Science; Polymer Science GA 566LF UT WOS:000275371000003 ER PT J AU Helms, D AF Helms, Douglas TI Hugh Hammond Bennett and the creation of the Soil Conservation Service SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article AB This article is a continuation of the historical developments discussed in "Hugh Hammond Bennett and the creation of the Soil Erosion Service," Journal of Soil and Water Conservation, Volume 64, Number 2, pages 68A-74A. The article discusses the events of September 19, 1933, to April 27, 1935, during which time Hugh Hammond Bennett and colleagues in the Soil Erosion Service (SES) established demonstration projects. The young agency weathered questions about their authority to work on private lands. The USDA and state agricultural institutions argued that this work belonged in USDA. Throughout the controversies, the cadre of soil conservationists won approval in the countryside and thereby built support in Congress for expansion of the soil conservation work on a permanent basis. The pending expiration of SES's emergency employment funding in June 1935 gave an air of urgency to legislation for a permanent agency. Finally, drought in the Great Plains and dust clouds sweeping eastward to the federal city dramatically demonstrated the need for soil conservation. C1 Nat Resources Conservat Serv, USDA, Washington, DC USA. RP Helms, D (reprint author), Nat Resources Conservat Serv, USDA, Washington, DC USA. NR 49 TC 6 Z9 6 U1 0 U2 3 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2010 VL 65 IS 2 BP 37A EP 47A DI 10.2489/jswc.65.2.37A PG 11 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 578II UT WOS:000276286500003 ER PT J AU Moriasi, DN Starks, PJ AF Moriasi, D. N. Starks, P. J. TI Effects of the resolution of soil dataset and precipitation dataset on SWAT2005 streamflow calibration parameters and simulation accuracy SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE calibration parameters; precipitation dataset resolution; simulation accuracy; Soil and Water Assessment Tool (SWAT); soil dataset resolution; streamflow ID EFFECTS ASSESSMENT PROJECT; WATER ASSESSMENT-TOOL; BENCHMARK WATERSHEDS; CONSERVATION; FLOW; SSURGO AB The resultant calibration parameter values find simulation accuracy of hydrologic models such is the 2005 Soil and Water Assessment Tool (SWAT2005) depend on how well spatial input parameters describe the characteristics of the study area. The objectives of this study were to (1) investigate the effect of soils dataset resolution (State Soil Geographic Database and Soil Survey Geographic Database) on SWAT2005 streamflow simulation performance and calibration parameters using four precipitation datasets and (2) determine the best combination of soil and precipitation datasets for the Cobb Creek, Lake Creek, and Willow Creek subwatersheds within the Fort Cobb Reservoir Experimental watershed, Oklahoma. SWAT2005 was calibrated and validated for streamflow for the three subwatersheds using the State Soil Geographic Database and the Soil Survey Geographic Database for each of the four available precipitation datasets with different spatial resolutions. The four sources of rainfall data included the National Weather Service's network of Cooperative Observer Program weather stations, statewide Oklahoma Mesonet, USDA Agricultural Research Service's weather station network (MICRONET), and National Weather Service Next Generation Radar (NEXRAD) precipitation estimates. The model performance was assessed using the Nash-Sutcliffe efficiency coefficient and percent bias statistics. During both the calibration and validation periods, there were no significant differences in the model monthly performance statistics between the higher resolution Soil Survey Geographic Database and the lower resolution State Soil Geographic Database across subwatersheds, irrespective of the rainfall dataset used. However, the model performed better when the NEXRAD and MICRONET precipitation datasets were used. There were slight to large differences in the resultant calibration parameter values depending on the calibration parameter, the precipitation data used, and the subwatershed. Large differences in the simulated surface runoff and deep aquifer recharge due to soils dataset resolution could lead to significant differences in the simulated water quality components such as sediments and nutrients. This is important because significant differences in simulated sediments and/or nutrients could lead to significantly different outcomes in terms of the impacts of a given conservation practice for studies like the Conservation Effects Assessment Project. Due to the lack of measured data to validate the simulated water balance components, it was recommended to use both the fine and coarse resolution soil datasets in combination with the finer spatial resolution precipitation datasets and the simulated water balance components of interest reported as a range. C1 [Moriasi, D. N.; Starks, P. J.] ARS, USDA, Grazinglands Res Lab, El Reno, OK USA. RP Moriasi, DN (reprint author), ARS, USDA, Grazinglands Res Lab, El Reno, OK USA. OI Moriasi, Daniel N/0000-0002-3389-3277 FU USDA NRCS FX The USDA NRCS is acknowledged for providing funds for this work through the support of the Conservation Effects Assessment Project-Watershed Assessment Studies. The authors are grateful to Alan Verser, a hydrologic technician for the USDA Agricultural Research Service Grazing lands Research Laboratory. El Reno, Oklahoma, Or his invaluable assistance with the SWAT2005 streamflow calibration and validation and the model performance evaluation. NR 44 TC 19 Z9 19 U1 6 U2 19 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2010 VL 65 IS 2 BP 63 EP 78 DI 10.2489/jswc.65.2.63 PG 16 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 578II UT WOS:000276286500008 ER PT J AU Bradburn, BN Aust, WM Dolloff, CA Cumbia, D Creighton, J AF Bradburn, B. N. Aust, W. M. Dolloff, C. A. Cumbia, D. Creighton, J. TI Evaluation of riparian forests established by the Conservation Reserve Enhancement Program (CREP) in Virginia SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE best management practices; natural regeneration; riparian buffers; water quality ID WATER-QUALITY; BUFFERS; SEDIMENT; FILTERS; RESTORATION; MANAGEMENT; HERBIVORY; DYNAMICS; WETLAND AB Forested riparian buffer strips are recommended as an agricultural best management practice for protection of water quality and for wildlife habitat. Since the 1990s, federal and state conservation agencies in Virginia have been involved in establishment of riparian buffers under the auspices of programs, such as the Conservation Reserve Enhancement Program (CREP). Riparian buffers established by the CREP program are considered to be beneficial towards the protection of water quality However, the benefit is based upon assumption that planted trees and shrubs survive and grow Little evaluation has been done within the state to document the success of the establishment efforts. We evaluated 63 riparian planting sites located in the Coastal Plain, Piedmont, and Ridge and Valley physiographic provinces of Virginia. Overall, the riparian forests in the Coastal Plain and Piedmont were fully stocked due to a combination of planted and natural regeneration. Riparian forests in the Ridge and Valley region were inadequately stocked, and the region has problems with invasive, exotic species. Major recommendations for improving the success of future riparian restoration;and establishment efforts are to (1) include the potential for natural regeneration when planning CREP plantings, (2) stop mechanized operations in the riparian buffers that reduce planted and natural regeneration (e.g., mowing and disking), (3) reduce herbivory (particularly cattle) within the riparian buffer, and (4) control invasive exotic species as necessary. C1 [Bradburn, B. N.; Aust, W. M.] Virginia Tech, Coll Nat Resources, Forest Resources & Environm Conservat Dept, Blacksburg, VA 24061 USA. [Dolloff, C. A.] US Forestry Serv, So Res Stn, Coldwater Fisheries Res Unit, Virginia Tech, Blacksburg, VA USA. [Cumbia, D.] Forest Resource Management Div, Charlottesville, VA USA. [Creighton, J.] Virginia Dept Forestry, Charlottesville, VA USA. RP Bradburn, BN (reprint author), Virginia Tech, Coll Nat Resources, Forest Resources & Environm Conservat Dept, Blacksburg, VA 24061 USA. FU Virginia Department of Forestry; USDA Forest Service; USDA Natural Resources Conservation Service; Virginia Polytechnic Institute and State University FX We wish to acknowledge the financial and logistical support of the Virginia Department of Forestry, USDA Forest Service, USDA Natural Resources Conservation Service, and Virginia Polytechnic Institute and State University. NR 32 TC 1 Z9 1 U1 2 U2 13 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2010 VL 65 IS 2 BP 105 EP 112 DI 10.2489/jswc.65.2.105 PG 8 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 578II UT WOS:000276286500011 ER PT J AU Sigua, GC Coleman, SW AF Sigua, Gilbert C. Coleman, Samuel W. TI Spatial distribution of soil carbon in pastures with cow-calf operation: effects of slope aspect and slope position SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Bahiagrass; Cow-calf; Soil carbon; Slope aspects; Slope positions; Subtropics ID RAIN-FOREST; COSTA-RICA; CATTLE; MANAGEMENT; VEGETATION; RANGELAND; SYSTEM; SITES; SIZE; TIME AB The rate at which soil carbon (C) accumulates in terrestrial beef agro-ecosystem is uncertain, as are the mechanisms responsible for the current C sink. Broad knowledge of cattle movement in pasture situations is critical to understanding their impact on agro-ecosystems. Movement of free-ranging cattle varies due to spatial arrangement of forage resources within pastures and the proximity of water, mineral feeders, and shades to grazing sites. The effects of slope aspect (SA) and slope position (SP) on nutrient dynamics in pastures are not well understood. Few studies have been made of soil-vegetation and soil-landscape relationships along an elevation gradient in tropical and subtropical regions. Current literature suggests no clear general relationships between grazing management and nutrient cycling. Early study reported no effect of grazing on soils nutrients, while other studies determined increases in soil nutrients due to grazing. We hypothesize that SA and SP could be of relative importance in controlling spatial variability of soil organic carbon (SOC). This study addressed the effects of SA and SP on the spatial distribution of SOC in forage-based pastures with cow-calf operation in subtropical region of southeastern USA. Soil samples were collected at 0-20 and 20-40 cm on contiguous south-, north-, east-, and west-facing slopes across different landscape positions (top slope, middle slope, and bottom slope) of 100 ha pastures during three summer seasons (2004-2006). Soil samples were air-dried, passed through a 2-mm mesh sieve, and visible roots were removed prior to analyses of SOC and other soil properties likely to affect spatial distribution of SOC. Analyses of soils were conducted at the Subtropical Agricultural Research Station in Brooksville, FL, following the dry-ash or the 'loss-on-ignition' method. Concentrations of organic carbon in soils from four different SA, three SP, and two soil depths (SD) in 2004, 2005, and 2006 were analyzed statistically following a four-way analysis of variance using the SAS PROC general linear model. There was an SA x SP interaction (p a parts per thousand currency signaEuro parts per thousand 0.0001) effect on the concentration of SOC. The two highest concentrations of SOC were observed from top slope (8.4 g kg(-1)) and middle slope (7.8 g kg(-1)) in south-facing slope, and the two lowest levels of SOC were in top slope (2.6 g kg(-1)) and middle slope (3.0 g kg(-1)) of north-facing slope, respectively. Soil C also varied significantly among SA (p a parts per thousand currency signaEuro parts per thousand 0.0001), SP (p a parts per thousand currency signaEuro parts per thousand 0.001), and SD (p a parts per thousand currency signaEuro parts per thousand 0.0001). Averaged across years and SP, soils on the south-facing slope contained the greatest amount of SOC, while soils on the north-facing slope had the least amount of SOC. Average concentrations of SOC in top slope and middle slope were comparable. These values were significantly (p a parts per thousand currency signaEuro parts per thousand 0.05) higher when compared with soils from bottom slope. About 73% of SOC spatial variability could be explained by total clay content. Concentrations of SOC were quadratically (SOC = 0.05 x clay(2) -aEuro parts per thousand 0.29 x clay + 4.4; p a parts per thousand currency signaEuro parts per thousand 0.001) related with total clay content. No other significant correlations between SOC and other soil properties were found. Our results have shown that soils on the south-facing slope had greatest concentration of SOC, while soils on the north-facing slope had the lowest concentration of SOC. The differences may be attributed to topographic aspect-induced microclimatic differences, which are causing differences in the biotic soil component and SOC trend. SA may be acting as an important topographic factor influencing local site microclimate mainly because it determines the amount of solar radiation received. Differences in microclimate are often linked to varying soil moisture and erosion potential and in turn could be used to explain distribution of plant communities. The north-facing slope had the lower forage availability when compared with the south-facing slope. There was a decreasing trend in the average forage availability with decreasing slope. Between the top slope and the bottom slope, forage availability declined from 2,484 to 1,448 kg ha(-1), which can be attributed to more grazing activities of cattle at bottom slope. Differences in SOC among different SA and SP could also be explained by varying amount of total clay. Concentrations of SOC were linearly related with increasing total clay content. The greatest amount of SOC was observed from soils located at the top slope of south-facing slope. Of the entire SA, south-facing slope had the greatest concentration of total clay, while the greatest clay content among SP was observed from the top slope. Results further revealed that 73% of SOC spatial variability could be explained by total clay content. The relationship between SOC and total clay content was best described by a quadratic equation: SOC = 0.05 x clay(2) -aEuro parts per thousand 0.29 x clay + 4.4; R (2) = 0.73; p a parts per thousand currency signaEuro parts per thousand 0.001. Results of our study are suggesting that SA and SP could be of relative importance in controlling spatial variability of SOC. Averaged across years, soils on the south-facing slope contained the greatest amount of SOC, while soils on the north-facing slope had the least amount of SOC. Based on the average concentration of SOC, the south-facing slope may have sequestered about 6,460 kg ha(-1) of SOC. Results have shown that landscape attributes (e.g., SA and SP) associated with beef cattle pastures as a part of the agro-ecological system could be potential sink for C sequestration, thus reducing atmospheric carbon dioxide concentrations. It is still critical to understand how the interactions of pasture management and landscape are affecting soil C dynamics. More studies are needed to assess the rate at which soil C is accumulating as well as the mechanisms responsible for the current and future C sink in forage-based pastures with cow-calf operations. C1 [Sigua, Gilbert C.; Coleman, Samuel W.] ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. RP Sigua, GC (reprint author), ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. EM gilbert.sigua@ars.usda.gov NR 32 TC 15 Z9 19 U1 5 U2 54 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 J9 J SOIL SEDIMENT JI J. Soils Sediments PD MAR PY 2010 VL 10 IS 2 BP 240 EP 247 DI 10.1007/s11368-009-0110-0 PG 8 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA 559UI UT WOS:000274853100012 ER PT J AU Lyons, JM Schwimer, JE Anthony, CT Thomson, JL Cundiff, JD Casey, DT Maccini, C Kucera, P Wang, YZ Boudreaux, JP Woltering, EA AF Lyons, John M., III Schwimer, Joshua E. Anthony, Catherine T. Thomson, Jessica L. Cundiff, Jason D. Casey, Douglas T. Maccini, Cynthia Kucera, Paul Wang, Yi-Zarn Boudreaux, J. Philip Woltering, Eugene A. TI The Role of VEGF Pathways in Human Physiologic and Pathologic Angiogenesis SO JOURNAL OF SURGICAL RESEARCH LA English DT Article DE angiogenesis; human; vascular endothelial growth factor (VEGF); platelet derived growth factor (PDGF); VEGF receptors; tyrosine kinases; mechanisms ID ENDOTHELIAL GROWTH-FACTOR; VASCULAR-PERMEABILITY FACTOR; IN-VITRO; CANCER-PATIENTS; CELLS SECRETE; TUMOR-GROWTH; PHASE-I; ANTIBODY; MODEL; BEVACIZUMAB AB Background. In preclinical models, VEGF is a potent stimulant of both physiologic and pathologic angiogenesis. Conversely, anti-VEGF regimens have successfully inhibited angiogenesis both in vitro and in vivo. We hypothesized that VEGF would stimulate both physiologic and pathologic angiogenesis in a human-based fibrin-thrombin clot angiogenesis assay. We further speculated that anti-VEGF regimens would inhibit angiogenesis in this assay. Methods. To test these hypotheses, discs of human placental veins (physiologic model) and fragments of human tumors (pathologic model) were embedded in fibrin-thrombin clots and treated with either VEG-F(A165) (VEGF) or anti-VEGF pathway reagents including bevacizumab, IMC-18F1, IMC-1121, and PTK787 (n = 30 wells per treatment group, multiple concentrations tested in each specimen). Angiogenic responses were assessed visually using a previously validated grading scheme. The percent of tissue explants that developed angiogenic invasion into the clot (%I) as well as the extent of angiogenic growth (Al) via a semi-quantitative scale were assessed at set intervals. Results. VEGF failed to stimulate angiogenesis in both the physiologic and the pathologic model. While anti-VEGF reagents that targeted only one element of the VEGF pathway failed to consistently inhibit angiogenesis, PTK787, a receptor tyrosine kinase inhibitor that targets multiple VEGF and non-VEGF receptors, profoundly inhibited both physiologic and pathologic angiogenesis. Conclusion. These results suggest that VEGF-related pathways may not be solely responsible for stimulating angiogenesis in humans. Targeting the VEGF pathway in combination with elements of other growth factor pathways may provide a more effective means of inhibiting angiogenesis than targeting VEGF alone. (C) 2010 Elsevier Inc. All rights reserved. C1 [Lyons, John M., III; Schwimer, Joshua E.; Anthony, Catherine T.; Cundiff, Jason D.; Casey, Douglas T.; Wang, Yi-Zarn; Boudreaux, J. Philip; Woltering, Eugene A.] Louisiana State Univ, Hlth Sci Ctr, Dept Surg, Sect Surg Oncol, Kenner, LA 70065 USA. [Lyons, John M., III; Schwimer, Joshua E.; Anthony, Catherine T.; Cundiff, Jason D.; Casey, Douglas T.; Wang, Yi-Zarn; Boudreaux, J. Philip; Woltering, Eugene A.] Louisiana State Univ, Hlth Sci Ctr, Dept Surg, Sect Surg Endocrinol, Kenner, LA 70065 USA. [Thomson, Jessica L.] USDA ARS, So Reg Res Ctr, Baton Rouge, LA USA. [Maccini, Cynthia; Kucera, Paul] Providence Canc Ctr, Portland, OR USA. [Woltering, Eugene A.] LSUHSC Stanley S Scott Canc Ctr, New Orleans, LA USA. RP Woltering, EA (reprint author), Louisiana State Univ, Hlth Sci Ctr, Dept Surg, Sect Surg Oncol, 200 W Esplanade Ste 200, Kenner, LA 70065 USA. EM ewolte@lsuhsc.edu NR 32 TC 12 Z9 15 U1 0 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-4804 J9 J SURG RES JI J. Surg. Res. PD MAR PY 2010 VL 159 IS 1 BP 517 EP 527 DI 10.1016/j.jss.2008.12.014 PG 11 WC Surgery SC Surgery GA 601NJ UT WOS:000278066600014 PM 19577260 ER PT J AU Mendoza, JA Watson, K Cullen, KW AF Mendoza, Jason A. Watson, Kathy Cullen, Karen Weber TI Change in Dietary Energy Density after Implementation of the Texas Public School Nutrition Policy SO JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION LA English DT Article ID WEIGHT-LOSS; BODY-WEIGHT; US ADULTS; LUNCH; OBESITY; WOMEN; FOODS; PARTICIPANTS; ASSOCIATION; CONSUMPTION AB Consumption of energy-dense foods has been associated with rising obesity rates and the metabolic syndrome. Reducing dietary energy density is an important strategy to address obesity, but few studies have examined the effect of nutrition policies on children's energy density. The study's objective was to assess the impact of the Texas Public School Nutrition Policy on children's energy density by using a pre- and post-policy evaluation. Analysis of variance/covariance and nonparametric tests compared energy density after the Texas policy change to intakes at baseline. Two years of lunch food records were collected from middle school students in Southeast Texas at three public middle schools: baseline (2001-2002) and 1 year after implementation of the Texas Policy (20052006). Students recorded the amount and source of foods consumed. The Texas Public School Nutrition Policy was designed to promote a healthy school environment by restricting portion sizes of high-fat and high-sugar snacks and sweetened beverages, fat content of foods, and serving of high-fat vegetables like french fries. Energy density (kcal/g): energy density-1 was the energy of foods only (no beverages) divided by the gram weight and has been previously associated with obesity and insulin resistance; energy density-2 included all food and beverages to give a complete assessment of all sources of calories. Following implementation of the Texas policy, students' energy density 1 significantly decreased from 2.80+/-1.08 kcal/g to 2.17+/-0.78 kcal/g (P<0.0001). Similarly, energy density-2 significantly decreased from 1.38+/-0.76 kcal/g to 1.29+/-0.53 kcal/g (P<0.0001). In conclusion, the Texas Public School Nutrition Policy was associated with desirable reductions in energy density, which suggests improved nutrient intake as a result of student school lunch consumption. J Am Diet Assoc. 2010;110:434-440. C1 [Mendoza, Jason A.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. [Mendoza, Jason A.; Cullen, Karen Weber] Baylor Coll Med, Dan L Duncan Canc Ctr, Houston, TX 77030 USA. RP Mendoza, JA (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM jason.mendoza@bcm.edu OI Mendoza, Jason/0000-0003-0833-4358 FU US Department of Agriculture (USDA) Economic Research Service [43-3AEM-2-80121]; USDA/Agricultural Research Service [143-3AEL-2-80121, 58-6250-6001, 6250-51000-047]; National Cancer Institute [CA88511, 1K07CA131178-01A1] FX This project has been funded in part by federal funds from the US Department of Agriculture (USDA) Economic Research Service (Cooperative Agreement 43-3AEM-2-80121) and USDA/Agricultural Research Service under Cooperative Agreements No. 143-3AEL-2-80121, 58-6250-6001, and 6250-51000-047. This work was also supported by a grant CA88511 from the National Cancer Institute (to K.W.C.). J.A.M. was supported in part by a career development award from the National Cancer Institute (1K07CA131178-01A1). The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 35 TC 33 Z9 34 U1 0 U2 9 PU AMER DIETETIC ASSOC PI CHICAGO PA 120 S RIVERSIDE PLZ, STE 2000, CHICAGO, IL 60606-6995 USA SN 0002-8223 J9 J AM DIET ASSOC JI J. Am. Diet. Assoc. PD MAR PY 2010 VL 110 IS 3 BP 434 EP 440 DI 10.1016/j.jada.2009.11.021 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 587LO UT WOS:000276994100015 PM 20184994 ER PT J AU Kiefer, MT Parker, MD Charney, JJ AF Kiefer, Michael T. Parker, Matthew D. Charney, Joseph J. TI Regimes of Dry Convection above Wildfires: Sensitivity to Fire Line Details SO JOURNAL OF THE ATMOSPHERIC SCIENCES LA English DT Article ID NUMERICAL SIMULATIONS; SQUALL LINES; DYNAMICS; MODEL; FIELD AB Fire lines are complex phenomena with a broad range of scales of cross-line dimension, undulations, and along-line variation in heating rates. While some earlier studies have examined parcel processes in two-dimensional simulations, the complexity of fire lines in nature motivates a study in which the impact of three-dimensional fire line details on parcel processes is examined systematically. This numerical modeling study aims to understand how fundamental processes identified in 2D simulations operate in 3D simulations where the fire line is neither straight nor uniform in intensity. The first step is to perform simulations in a 3D model, with no fire line undulations or inhomogeneity. In general, convective modes simulated in the 2D model are reproduced in the 3D model. In one particular case with strong vertical wind shear, new convection develops separate from the main line of convection as a result of local changes to parcel speed and heating. However, in general the processes in the 2D and 3D simulations are identical. The second step is to examine 3D experiments wherein fire line shape and along-line inhomogeneity are varied. Parcel heating, as well as convective mode, is shown to exhibit sensitivity to fire line shape and along-line inhomogeneity. C1 [Kiefer, Michael T.; Parker, Matthew D.] N Carolina State Univ, Raleigh, NC 27695 USA. [Charney, Joseph J.] US Forest Serv, No Res Stn, USDA, E Lansing, MI USA. RP Kiefer, MT (reprint author), 1407 S Harrison Rd,Room 220, E Lansing, MI 48823 USA. EM mtkiefer@msu.edu RI Parker, Matthew/A-5156-2008; OI Kiefer, Michael/0000-0002-4824-0148 FU National Defense Science and Engineering Graduate (NDSEG) FX The first author wishes to thank Dr. Yuh-Lang Lin for proposing the idea of a parameter space study of dry forest fire convection. In addition, we wish to thank George Bryan and three anonymous reviewers for their helpful comments. The first author was supported by a National Defense Science and Engineering Graduate (NDSEG) fellowship while performing this research. Computing was performed on the NCSU PAMS Linux cluster and the Army Research Laboratory Linux cluster (MJM). NR 34 TC 1 Z9 1 U1 0 U2 2 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0022-4928 J9 J ATMOS SCI JI J. Atmos. Sci. PD MAR PY 2010 VL 67 IS 3 BP 611 EP 632 DI 10.1175/2009JAS3226.1 PG 22 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 575WS UT WOS:000276101400004 ER PT J AU McKinnish, T Walsh, R White, TK AF McKinnish, Terra Walsh, Randall White, T. Kirk TI Who gentrifies low-income neighborhoods? SO JOURNAL OF URBAN ECONOMICS LA English DT Article DE Gentrification; Demographics; Neighborhood change; Residential mobility; Displacement ID DISPLACEMENT; SEGREGATION; SUCCESSION; DYNAMICS; 1990S; MODEL AB This paper uses confidential Census data, specifically the 1990 and 2000 Census Long Form data, to study demographic processes in neighborhoods that gentrified during the 1990s. In contrast to previous studies, the analysis is conducted at the more refined census-tract level, with a narrower definition of gentrification and more closely matched comparison neighborhoods. Furthermore, our access to individual-level data with census tract identifiers allows us to separately identify recent in-migrants and long-term residents. Our results indicate that, on average, the demographic flows associated with the gentrification of urban neighborhoods during the 1990s are not consistent with displacement and harm to minority households. In fact, taken as a whole, our results suggest that gentrification of predominantly black neighborhoods creates neighborhoods that are attractive to middle-class black households. (C) 2009 Elsevier Inc. All rights reserved. C1 [McKinnish, Terra] Univ Colorado, Boulder, CO 80309 USA. [Walsh, Randall] Univ Pittsburgh, Pittsburgh, PA 15260 USA. [White, T. Kirk] ERS, USDA, Washington, DC 20036 USA. RP McKinnish, T (reprint author), Univ Colorado, UCB 256, Boulder, CO 80309 USA. EM mckinnis@colorado.edu RI Freeman, Lance/B-8774-2009 FU NICHD NIH HHS [R03 HD053860-02, R03 HD053860] NR 18 TC 41 Z9 41 U1 5 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0094-1190 J9 J URBAN ECON JI J. Urban Econ. PD MAR PY 2010 VL 67 IS 2 BP 180 EP 193 DI 10.1016/j.jue.2009.08.003 PG 14 WC Economics; Urban Studies SC Business & Economics; Urban Studies GA 548ML UT WOS:000273967200002 PM 20161532 ER PT J AU Ridpath, JF Fulton, RW Kirkland, PD Neill, JD AF Ridpath, Julia F. Fulton, Robert W. Kirkland, Peter D. Neill, John D. TI Prevalence and antigenic differences observed between Bovine viral diarrhea virus subgenotypes isolated from cattle in Australia and feedlots in the southwestern United States SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Antigenic diversity; Bovine viral diarrhea virus; pestivirus; prevalence; subgenotype ID GENETIC DIVERSITY; MANNHEIMIA-HAEMOLYTICA; NEUTRALIZING ACTIVITY; NORTH-AMERICA; BVDV STRAINS; SOUTH-AFRICA; ANTIBODIES; CALVES; DISEASE; SPECIFICITY AB Bovine viral diarrhea virus (BVDV) is divided into 2 different species within the Pestivirus genus, BVDV type 1 (BVDV-1) and BVDV type 2 (BVDV-2). Further phylogenetic analysis has revealed subgenotype groupings within the 2 types. Thus far, 12 BVDV-1 subgenotypes (a-l) and 2 BVDV-2 subgenotypes (a and b) have been identified. The purpose of the current study was to determine the prevalence of BVDV subgenotypes in the United States and Australia and to determine if there are detectable antigenic differences between the prevalent subgenotypes. To determine prevalence, phylogenetic analysis was performed on 2 blinded panels of isolates consisting of 351 viral isolates provided by the Elizabeth Macarthur Laboratory, New South Wales, and 514 viral isolates provided by Oklahoma State University. Differences were observed in the prevalence of BVDV subgenotypes between the United States (BVDV-1b most prevalent subgenotype) and Australia (BVDV-1c most prevalent subgenotype). To examine antigenic differences between the subgenotypes identified in samples from the United States and Australia, polyclonal antisera was produced in goats by exposing them at 3-week intervals to 2 noncytopathic and 1 cytopathic strain of either BVDV-la, BVDV-lb, BVDV-1c. BVDV-2a, or Border disease virus (BDV). Virus neutralization (VN) assays were then performed against 3 viruses from each of the 5 subgenotypes. Comparison of VN results suggests that there are antigenic differences between BVDV strains belonging to different subgenotypes. The present study establishes a foundation for further studies examining whether vaccine protection can be improved by basing vaccines on the BVDV subgenotypes prevalent in the region in which the vaccine is to be used. C1 [Ridpath, Julia F.; Neill, John D.] ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Fulton, Robert W.] Oklahoma State Univ, Dept Vet Pathobiol, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA. [Kirkland, Peter D.] Elizabeth Macarthur Agr Inst, Virol Lab, Menangle, NSW, Australia. RP Ridpath, JF (reprint author), ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM julia.ridpath@ars.usda.gov NR 47 TC 50 Z9 50 U1 1 U2 12 PU AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC PI TURLOCK PA PO BOX 1522, TURLOCK, CA 95381 USA SN 1040-6387 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAR PY 2010 VL 22 IS 2 BP 184 EP 191 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 571FG UT WOS:000275736400003 PM 20224075 ER PT J AU Pithua, P Wells, SJ Godden, SM Sreevatsan, S Stabel, JR AF Pithua, Patrick Wells, Scott J. Godden, Sandra M. Sreevatsan, Srinand Stabel, Judith R. TI Experimental validation of a nested polymerase chain reaction targeting the genetic element ISMAP02 for detection of Mycobacterium avium subspecies paratuberculosis in bovine colostrum SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Bovine colostrum; ISMAP02; Mycobacterium avium subsp. paratuberculosis; nested polymerase chain reaction ID CHLAMYDIA-PNEUMONIAE; JOHNES-DISEASE; FECAL CULTURE; PCR; DIAGNOSIS; ELISA; MILK; COWS; DNA; RELIABILITY AB Colostrum samples experimentally inoculated with Mycobacterium (triton subsp. paratuberculosis (MAP; strain K-10) at increasing concentrations between 1 x 10(1) and 1 x 10(9) cells/ml were tested for recovery of MAP DNA using a nested ISMAP02 target polymerase chain reaction initially developed for detecting MAP DNA in fecal samples. The following detection rates were achieved for sample replicates inoculated with unsonicated MAP pure stock: 100% between 1 x 10(7) and 1 x 10(9) cells/ml, 75% between 1 x 10(3) and 1 x 10(6) cells/ml, and 50% between 1 x 10(1) and 1 x 10(2) cells/ml replicates. Detection rates achieved for the colostrum sample replicates inoculated with sonicated MAP cell suspension were 75% for 1 x 10(9) cells/ml. 100% between 1 x 10(7) and 1 x 10(8) cells/ml, 75% for 1 x 10(6) cells/ml, 0 for 1 x 10(4) cells/ml, and 25% between 1 x 10(1) and 1 x 10(3) cells/ml. When negative control colostrum samples were tested, 16 of 18 (89%) samples were correctly detected as negative for MAP DNA using the current assay. In conclusion, the MAP DNA detection rates of the present assay improved with increasing concentrations of MAP in the colostrum sample replicates. although MAP DNA was also detected in 2 of 18 (11%) negative control samples, suggesting an undefined technical problem with the assay or, perhaps, sample contamination during preparation. Overall, the present findings suggest a potential role of the proposed polymerase chain reaction assay to detect MAP in colostrum. However, adoption of this test for use in routine screening of field colostrum for MAP awaits findings from an ongoing field validation study. C1 [Pithua, Patrick; Wells, Scott J.; Godden, Sandra M.; Sreevatsan, Srinand] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. [Stabel, Judith R.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Pithua, P (reprint author), Univ Missouri, Coll Vet Med, Dept Vet Med & Surg, 900 E Campus Dr, Columbia, MO 65211 USA. EM pithua@missouri.edu RI Pithua, Patrick/J-2426-2013; OI Pithua, Patrick/0000-0002-2817-5772; Sreevatsan, Srinand/0000-0002-5162-2403 NR 17 TC 8 Z9 8 U1 0 U2 1 PU AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC PI TURLOCK PA PO BOX 1522, TURLOCK, CA 95381 USA SN 1040-6387 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAR PY 2010 VL 22 IS 2 BP 253 EP 256 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 571FG UT WOS:000275736400015 PM 20224087 ER PT J AU Lorusso, A Faaberg, KS Killian, ML Koster, L Vincent, AL AF Lorusso, Alessio Faaberg, Kay S. Killian, Mary Lea Koster, Leo Vincent, Amy L. TI One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Swine influenza; Pigs; Real-time RT-PCR; Pandemic (H1N1); Diagnosis ID QUANTITATION; PIGS; HEMAGGLUTININ; INFECTION; DOGS; RNA AB In the spring of 2009, a novel (H1N1) influenza A virus began to spread among humans worldwide. Although the 2009 H1N1 is related genetically to swine influenza viruses, human infection has not been connected to pig exposure. Because the virus is now circulating widely in the human population, swine herds are at increased risk of becoming infected. In order to investigate potential outbreaks of the 2009 pandemic virus in pigs, a quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) for the detection of the (H1N1) 2009 RNA in clinical specimens was developed. To evaluate the applicability of the test as a diagnostic tool in the screening of field specimens from swine, 64 field isolates of North American swine, 5 equine and 48 avian influenza viruses collected during diagnostic investigations were analyzed retrospectively as well as samples collected during an experimental in vivo infection with two novel H1N1 isolates, A/California/04/2009 (H1N1)v virus and A/Mexico/4108/2009 (H1N1)v. The sensitivity of the qRT-PCR was shown to be higher with respect to standard techniques such as virus isolation and the reproducibility was satisfactory. The present unique and highly sensitive assay is able to detect as little as 1 x 10(1) copies of RNA per mu l of template and it represents a rapid and useful approach for the screening and quantitation of (H1N1) 2009 RNA in porcine specimens. Published by Elsevier B.V. C1 [Lorusso, Alessio; Faaberg, Kay S.; Vincent, Amy L.] USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Killian, Mary Lea; Koster, Leo] USDA APHIS, Diagnost Virol Lab, Natl Vet Serv Lab, Ames, IA USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, POB 70,1920 Dayton Ave, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov RI Lorusso, Alessio/A-7311-2016; OI Lorusso, Alessio/0000-0001-7933-7367; Lorusso, Alessio/0000-0001-6156-8212 FU USDA-ARS; DHHS-CDC FX The authors thank Michelle Harland and David Michael for technical assistance and Dr. Becky Jepsen, Brian Pottebaum and Jason Huegel for assistance with animal studies. Isolates of 2009 pandemic H1N1 were generously provided by Dr. Alexander Klimov (CDC) and endemic swine influenza virus isolates provided by Dr. Marie Gramer (University of Minnesota). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Funding was provided by USDA-ARS and DHHS-CDC. NR 17 TC 25 Z9 27 U1 0 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 J9 J VIROL METHODS JI J. Virol. Methods PD MAR PY 2010 VL 164 IS 1-2 BP 83 EP 87 DI 10.1016/j.jviromet.2009.12.002 PG 5 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA 585JD UT WOS:000276820500014 PM 20005904 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD USA. [Gipson, Chester] USDA, APHIS, AC, Washington, DC USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Bethesda, MD USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 J9 LAB ANIMAL JI Lab Anim. PD MAR PY 2010 VL 39 IS 3 BP 68 EP 68 DI 10.1038/laban0310-68b PG 1 WC Veterinary Sciences SC Veterinary Sciences GA 564TX UT WOS:000275241600008 PM 20164942 ER PT J AU Prasad, AM Iverson, LR Peters, MP Bossenbroek, JM Matthews, SN Sydnor, TD Schwartz, MW AF Prasad, Anantha M. Iverson, Louis R. Peters, Matthew P. Bossenbroek, Jonathan M. Matthews, Stephen N. Sydnor, T. Davis Schwartz, Mark W. TI Modeling the invasive emerald ash borer risk of spread using a spatially explicit cellular model SO LANDSCAPE ECOLOGY LA English DT Article DE Emerald ash borer; EAB; Agrilus planipennis; Spread model; Stratified dispersal; Spatially explicit cellular model Ohio; Gravity model; Fraxinus; Ash; Roads networks; Invasive; Highway traffic; Insect flight model; Insect ride model ID LONG-DISTANCE DISPERSAL; EASTERN UNITED-STATES; BIOLOGICAL INVASIONS; AGRILUS-PLANIPENNIS; POPULATION SPREAD; CLIMATE-CHANGE; NORTH-AMERICA; COLEOPTERA; ECOLOGY; STOCHASTICITY AB The emerald ash borer (EAB, Agrilus planipennis) is decimating native ashes (Fraxinus sp.) throughout midwestern North America, killing millions of trees over the years. With plenty of ash available throughout the continent, the spread of this destructive insect is likely to continue. We estimate that the insect has been moving along a "front" at about 20 km/year since about 1998, but more alarming is its long-range dispersal into new locations facilitated by human activities. We describe a spatially explicit cell-based model used to calculate risk of spread in Ohio, by combining the insect's flight and short-range dispersal ("insect flight") with human-facilitated, long-range dispersal ("insect ride"). This hybrid model requires estimates of EAB abundance, ash abundance, major roads and traffic density, campground size and usage, distance from the core infested zone, wood products industry size and type of wood usage, and human population density. With the "insect flight" model, probability of movement is dependent on EAB abundance in the source cells, the quantity of ash in the target cells, and the distances between them. With the "insect-ride" model, we modify the value related to ash abundance based on factors related to potential human-assisted movements of EAB-infested ash wood or just hitchhiking insects. We attempt to show the advantage of our model compared to statistical approaches and to justify its practical value to field managers working with imperfect knowledge. We stress the importance of the road network in distributing insects to new geographically dispersed sites in Ohio, where 84% were within 1 km of a major highway. C1 [Prasad, Anantha M.; Iverson, Louis R.; Peters, Matthew P.; Matthews, Stephen N.] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. [Bossenbroek, Jonathan M.] Univ Toledo, Dept Environm Sci, Oregon, OH 43618 USA. [Bossenbroek, Jonathan M.] Univ Toledo, Lake Erie Ctr, Oregon, OH 43618 USA. [Sydnor, T. Davis] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Schwartz, Mark W.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. RP Prasad, AM (reprint author), US Forest Serv, USDA, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM aprasad@fs.fed.us; liverson@fs.fed.us; mpeters@fs.fed.us; Jonathan.bossenbroek@utoledo.edu; snmatthews@fs.fed.us; sydnor.1@osu.edu; mwschwartz@ucdavis.edu RI Bossenbroek, Jonathan/F-3664-2011; Schwartz, Mark/G-1066-2011; Bossenbroek, Jonathan/C-9184-2012; Matthews, Stephen/D-1050-2012; Iverson, Louis/C-7554-2009 OI Schwartz, Mark/0000-0002-3739-6542; Iverson, Louis/0000-0001-9501-471X FU PREISM Program of the USDA FX Thanks to Lindsey Vest of the Ohio Department of Agriculture for providing data on EAB infestation locations. We are grateful to Elizabeth LaPoint from the FIA GIS Support Center for overlaying the Ohio FIA plots with the Ohio GAP data to estimate ash BA. We thank the Ohio Center for Mapping, especially Lawrence Spencer, for creating and providing the Ohio GAP data. We thank Mary Brown, FHWA Office of Highway Policy Information, for providing traffic data. Thanks to Dan Kashian, Denys Yemshanov, John Pedlar and Rueben Keller for the friendly reviews and the two anonymous reviewers, and John Stanovich for the statistical review. We thank Lucy Burde for editing the manuscript. This work was funded in part by the PREISM Program of the USDA (awarded to JMB & LRI). This is publication No. 200X-XXX from the University of Toledo Lake Erie Center. NR 52 TC 56 Z9 61 U1 8 U2 82 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAR PY 2010 VL 25 IS 3 BP 353 EP 369 DI 10.1007/s10980-009-9434-9 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA 563IR UT WOS:000275122600003 ER PT J AU Bigelow, SW Parks, SA AF Bigelow, Seth W. Parks, Sean A. TI Predicting altered connectivity of patchy forests under group selection silviculture SO LANDSCAPE ECOLOGY LA English DT Article DE Fragmentation; Percolation; Spatial pattern analysis; Sierra Nevada East-side pine; Group selection; Silviculture; Connectivity; Neutral map ID AMERICAN MARTEN; LANDSCAPE CONNECTIVITY; HABITAT FRAGMENTATION; CONSERVATION BIOLOGY; PERCOLATION THEORY; SIERRA-NEVADA; PATTERNS; CALIFORNIA; PRINCIPLES; BEHAVIOR AB Group selection silviculture creates canopy openings that can alter connectivity in patchy forests, thereby affecting wildlife movement and fire behavior. We examined effects of group selection silviculture on percolation (presence of continuously forested routes across a landscape) in Sierra Nevada East-side pine forest in northern California, USA. Four similar to 250 ha project areas were analyzed at three map resolutions in three ways: analyzing forest cover maps for percolation before and after group-selection treatment, placing simulated group openings in forest cover maps until fragmentation occurred, and comparing project areas to neutral maps that varied in forest cover and self-adjacency. Two project areas were fragmented (i.e., did not percolate) prior to treatment, one resisted fragmentation, and the other became fragmented by treatment when analyzed at 30 m cell resolution. Median simulated openings required to create fragmentation agreed well with the actual number. There was a well-defined transition between percolating and non-percolating neutral maps; increased aggregation of forest lowered the critical value at which forests percolated. A logistic model based on these maps predicted percolation behavior of the project areas effectively, but alternative generating algorithms gave slightly different predictions. A graph of this model provides a straightforward way to visualize how close a landscape is to fragmentation based on its forest cover and aggregation. In East-side Sierran landscape, fragmentation from group-selection openings may make the landscape less hospitable to the American marten but more resistant to crown fire. C1 [Bigelow, Seth W.] US Forest Serv, Pacific SW Res Stn, USDA, Davis, CA 95618 USA. [Parks, Sean A.] US Forest Serv, Aldo Leopold Res Inst, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. RP Bigelow, SW (reprint author), US Forest Serv, Pacific SW Res Stn, USDA, 1731 Res Pk Dr, Davis, CA 95618 USA. EM sbigelow@fs.fed.us FU Joint Fire Sciences Program; Region 5 of The Forest Service FX Patti Millet of the Plumas National Forest staff encouraged our interest in group selection silviculture in East-side pine types. Wendell Cropper suggested simulating placement of group selection openings. Programming assistance was provided by the True Basic users' forum and Lora Murphy. Michael Papaik and two anonymous reviewers provided comments that improved the manuscript. Research was funded by the Joint Fire Sciences Program and Region 5 of The Forest Service. NR 58 TC 1 Z9 1 U1 3 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAR PY 2010 VL 25 IS 3 BP 435 EP 447 DI 10.1007/s10980-009-9421-1 PG 13 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA 563IR UT WOS:000275122600009 ER PT J AU Weiss, SR Tenney, JM Thomson, JL Anthony, CT Chiu, ES Friedlander, PL Woltering, EA AF Weiss, Sean R. Tenney, Justin M. Thomson, Jessica L. Anthony, Catherine T. Chiu, Ernest S. Friedlander, Paul L. Woltering, Eugene A. TI The Effect of AlloDerm on the Initiation and Growth of Human Neovessels SO LARYNGOSCOPE LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Head-and-Neck-Society/Research Workshop on the Biology, Prevention and Treatment of Head and Neck Cancer CY AUG 17-20, 2006 CL Chicago, IL SP Amer Head & Neck Soc DE AlloDerm; angiogenesis; wound healing; skin ID SMALL-INTESTINAL SUBMUCOSA; IN-VITRO ASSAY; EXTRACELLULAR-MATRIX; ANGIOGENESIS ASSAY; ACELLULAR DERMIS; RECONSTRUCTION; ALLOGRAFT; BIOSCAFFOLD; SCAFFOLD; REPAIR AB Objectives/Hypothesis: AlloDerm (LifeCell Corp., Branchburg, NJ) is commonly employed for reconstruction of ablative soft tissue and mucosal defects following surgical resections. Although devoid of growth factors, AlloDerm may serve as an adhesive matrix for binding of growth factors, increasing local angiogenesis, and wound healing. We hypothesized that AlloDerm would enhance angiogenesis and might be altered with autologous blood products to enhance initiation of the angiogenic response. Methods: We used a human placental vein in a fibrin-thrombin clot-based angiogenesis model. Four groups, human placental vein (HPVM), HPVM with AlloDerm, HPVM with AlloDerm plus platelet-poor plasma, and HPVM with AlloDerm plus platelet-rich plasma were evaluated. Endothelial cell growth was evaluated visually (40x). Hematoxylin and eosin staining and immunofluorescent staining for growth within the AlloDerm matrix were also performed. To assess human umbilical vein endothelial cell (HUVEC) sites of attachment to AlloDerm, we incubated HUVEC cells with AlloDerm for a period of 2 weeks and evaluated attachment with anti-factor VIII immunofluorescence. Results: Angiogenic initiation decreased in the combined placental vein with AlloDerm group (P<.0001 at day 7, 14, 21). Additionally, initiation in the AlloDerm plus platelet-poor plasma group was significantly better than the AlloDerm alone group when placentas 2 and 3 were compared (P<.0001). On hematoxylin and eosin staining and immunofluorescent factor VIII staining, no endothelial growth into the AlloDerin was noted in the samples analyzed. Conclusions: AlloDerm may be enriched with platelet-poor plasma to stimulate greater initiation and wound healing; however, AlloDerm inhibits angiogenic initiation in this model. C1 [Weiss, Sean R.; Tenney, Justin M.] Louisiana State Univ, Dept Otorhinolaryngol Head & Neck Surg, Hlth Sci Ctr, New Orleans, LA 70112 USA. [Anthony, Catherine T.; Chiu, Ernest S.; Woltering, Eugene A.] Louisiana State Univ, Dept Surg, Hlth Sci Ctr, New Orleans, LA 70112 USA. [Thomson, Jessica L.] Agr Res Serv, USDA, So Reg Res Ctr, Baton Rouge, LA USA. [Friedlander, Paul L.] Tulane Univ, Hlth Sci Ctr, Dept Otolaryngol, New Orleans, LA 70118 USA. RP Tenney, JM (reprint author), Louisiana State Univ, Dept Otolaryngol Head & Neck Surg, Hlth Sci Ctr, 533 Bolivar St, New Orleans, LA 70112 USA. EM jtenne@lsuhse.edu NR 22 TC 2 Z9 2 U1 1 U2 2 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0023-852X J9 LARYNGOSCOPE JI Laryngoscope PD MAR PY 2010 VL 120 IS 3 BP 443 EP 449 DI 10.1002/lary.20679 PG 7 WC Medicine, Research & Experimental; Otorhinolaryngology SC Research & Experimental Medicine; Otorhinolaryngology GA 564OI UT WOS:000275224200001 PM 20131371 ER PT J AU Guo, WC Wang, SJ Tiwari, G Johnson, JA Tang, J AF Guo, Wenchuan Wang, Shaojin Tiwari, Gopal Johnson, Judy A. Tang, Juming TI Temperature and moisture dependent dielectric properties of legume flour associated with dielectric heating SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Dielectric constant; Loss factor; Microwave; Radio frequency; Postharvest ID RADIO-FREQUENCY TREATMENTS; INSECT CONTROL; PHYSICAL-PROPERTIES; MICROWAVE TREATMENTS; PRODUCT QUALITY; FOOD MATERIALS; LENTIL SEEDS; BULK-DENSITY; RICE; FRUITS AB Dielectric properties data are important in developing thermal treatments using radio frequency (RF) and microwave (MW) energy and are essential in estimating heating uniformity in electromagnetic fields. Dielectric properties of flour samples from four legumes (chickpea, green pea, lentil, and soybean) at four different moisture contents were measured with an open-ended coaxial probe and impedance analyzer at frequencies of 10-1800 MHz and temperatures of 20-90 degrees C. The dielectric constant and loss factor of the legume samples decreased with increasing frequency but increased with increasing temperature and moisture content. At low frequencies and high temperatures and moisture contents, negative linear correlations were observed between the loss factor and frequency on a log-log plot, which was mainly caused by the ionic conductance. At 1800 MHz, the dielectric properties data could be used to estimate the legume sample density judging from high linear correlations. Loss factors for the four legume samples were similar at 27 MHz, 20 degrees C and low moisture contents (e.g. <15 g/100 g). At the highest moisture content (e.g. 20 g/100 g) soybean had the highest loss factor at 27 MHz and 20 degrees C, followed by lentil, green pea, and chickpea. The difference in loss factor among the four legumes did not show clear patterns at 915 MHz. Deep penetration depths at 27 MHz could help in developing large-scale industrial RF treatments for postharvest insect control or other applications that require bulk heating in legumes with acceptable heating uniformity and throughputs. (C) 2009 Published by Elsevier Ltd. C1 [Wang, Shaojin; Tiwari, Gopal; Tang, Juming] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Guo, Wenchuan] NW A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Johnson, Judy A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Tang, J (reprint author), Washington State Univ, Dept Biol Syst Engn, 213 LJ Smith Hall, Pullman, WA 99164 USA. EM jtang@wsu.edu RI Wang, Shaojin/C-4968-2012; Tang, Juming/A-4027-2009 OI Tang, Juming/0000-0001-9449-1004 FU USDA-NRI [2005-35503-16223]; USDA-CSREES [2008-34103-19091] FX The research was conducted while Dr. Wenchuan Guo was a visiting scholar at Department of Biological Systems Engineering, Washington State University (WSU), and supported by grants from USDA-NRI (2005-35503-16223) and USDA-CSREES (2008-34103-19091). We thank Dr. S. Trabelsi (USDA-ARS, Athens, GA) for reviewing this manuscript and providing constructive suggestions, Mr. W. DeWitt (Biological Systems Engineering, WSU) for developing the compressor used for compressing legume flour, Dr. J. Zhang (Civil and Environmental Engineering, WSU) for helping in compressing samples, and Mrs. G. Mikhaylenko (Biological Systems Engineering, WSU) for technical assistance in dielectric properties measurements. NR 45 TC 52 Z9 65 U1 4 U2 54 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD MAR PY 2010 VL 43 IS 2 BP 193 EP 201 DI 10.1016/j.lwt.2009.07.008 PG 9 WC Food Science & Technology SC Food Science & Technology GA 525FA UT WOS:000272198400001 ER PT J AU Bujanovic, B Ralph, S Reiner, R Hirth, K Atalla, R AF Bujanovic, Biljana Ralph, Sally Reiner, Richard Hirth, Kolby Atalla, Rajai TI Polyoxometalates in Oxidative Delignification of Chemical Pulps: Effect on Lignin SO MATERIALS LA English DT Review DE polyoxometalates; keggin type; lignin; delignification; aerobic; anaerobic; lignin reactions; products ID TRANSITION-METAL CATALYSIS; WATER-SOLUBLE SALTS; OXYGEN DELIGNIFICATION; KRAFT PULP; MODEL COMPOUNDS; MOLYBDOVANADOPHOSPHATE POLYANION; RESIDUAL LIGNINS; WOOD LIGNIN; POM ANIONS; PART II AB Chemical pulps are produced by chemical delignification of lignocelluloses such as wood or annual non-woody plants. After pulping (e. g., kraft pulping), the remaining lignin is removed by bleaching to produce a high quality, bright paper. The goal of bleaching is to remove lignin from the pulp without a negative effect on the cellulose; for this reason, delignification should be performed in a highly selective manner. New environmentally-friendly alternatives to conventional chlorine-based bleaching technologies (e. g., oxygen, ozone, or peroxide bleaching) have been suggested or implemented. In an attempt to find inorganic agents that mimic the action of highly selective lignin-degrading enzymes and that can be applicable in industrial conditions, the researchers have focused on polyoxometalates (POMs), used either as regenerable redox reagents (in anaerobic conditions) or as catalysts (in aerobic conditions) of oxidative delignification. The aim of this paper is to review the basic concepts of POM delignification in these two processes. C1 [Bujanovic, Biljana] SUNY ESF, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA. [Ralph, Sally; Reiner, Richard; Hirth, Kolby] USDA Forest Serv, Forest Prod Lab, Madison, WI USA. [Atalla, Rajai] Cellulose Sci Int, Madison, WI USA. RP Bujanovic, B (reprint author), SUNY ESF, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA. EM bbujanovic@esf.edu; sralph@fs.fed.us; rreiner@fs.fed.us; khirth@fs.fed.us; rhatalla@celscint.com NR 45 TC 12 Z9 12 U1 3 U2 39 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1996-1944 J9 MATERIALS JI Materials PD MAR PY 2010 VL 3 IS 3 BP 1888 EP 1903 DI 10.3390/ma3031888 PG 16 WC Materials Science, Multidisciplinary SC Materials Science GA 864LC UT WOS:000298240500020 ER PT J AU Reeves, WK Peiris, KHS Scholte, EJ Wirtz, RA Dowell, FE AF Reeves, W. K. Peiris, K. H. S. Scholte, E. -J. Wirtz, R. A. Dowell, F. E. TI Age-grading the biting midge Culicoides sonorensis using near-infrared spectroscopy SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Culicoides; age-grading; bluetongue; near-infrared spectroscopy ID VIRUS; TRANSMISSION; VARIIPENNIS; CERATOPOGONIDAE; DIPTERA AB Age-grading of insects is important in the control and monitoring of both insect populations and vector-borne diseases. Microscopy and morphological techniques exist to age-grade most blood-feeding flies, but these techniques are laborious, often destructive to the insects, and slow. Near-infrared spectroscopy (NIRS) can be automated and is a non-destructive technique for age-grading. We applied NIRS techniques to age-grade females of the biting midge, Culicoides sonorensis Wirth & Jones (Diptera: Ceratopogonidae), the vector of bluetongue and other arboviruses in North America. Female flies of five known age cohorts (1, 3, 6, 9 and 12 days post-emergence) from three laboratory colonies were used. The data indicate that NIRS can be used to differentiate age groups of C. sonorensis. C1 [Dowell, F. E.] ARS, USDA, Ctr Grain & Anim Hlth Res, Engn & Wind Eros Res Unit, Manhattan, KS 66502 USA. [Reeves, W. K.] ARS, USDA, Arthropod Borne Anim Dis Res Lab, Laramie, WY USA. [Peiris, K. H. S.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Scholte, E. -J.] Natl Reference Lab Fytosanit Pests & Dis Plant Pr, Wageningen, Netherlands. [Wirtz, R. A.] CDC, Entomol Branch, Atlanta, GA 30333 USA. RP Dowell, FE (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Engn & Wind Eros Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM floyd.dowell@ars.usda.gov NR 17 TC 9 Z9 9 U1 0 U2 6 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0269-283X J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD MAR PY 2010 VL 24 IS 1 BP 32 EP 37 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 555CG UT WOS:000274484100005 PM 20377729 ER PT J AU Mauget, S De Pauw, E AF Mauget, Steve De Pauw, Eddy TI The ICARDA Agro-Climate tool SO METEOROLOGICAL APPLICATIONS LA English DT Article DE ICARDA; crop evapotranspiration; agro-climatology; Visual Basic AB A Visual Basic agro-climate application developed by climatologists at the International Center tier Agricultural Research in the Dry Areas (ICARDA) and the U.S Department of Agriculture is described here The applications climate database consists of Weather generator parameters derived from the daily data of 649 meteorological stations in file ICARDA mandate region From those parameters the program calculates climate statistics over arbitrarily defined periods within summer Or winter growing seasons at user-selected latitude-longitude coordinates The statistics reported include crop evapotranspiration estimates derived from the FAO-56 single crop coefficient algorithm, probabilities of exceedence of both cumulative rainfall and growing degree days. the probability that minimum and maximum dally temperatures will exceed user-defined temperature thresholds and the probability of heat stress, cold stress and dry periods of varying duration Copyright (C) 2009 Royal Meteorological Society C1 [Mauget, Steve] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [De Pauw, Eddy] Int Ctr Agr Res Dry Areas, Aleppo, Syria. RP Mauget, S (reprint author), ARS, USDA, Cropping Syst Res Lab, 3310 4th St, Lubbock, TX 79415 USA. NR 12 TC 2 Z9 2 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1350-4827 EI 1469-8080 J9 METEOROL APPL JI Meteorol. Appl. PD MAR PY 2010 VL 17 IS 1 BP 105 EP 116 DI 10.1002/met.165 PG 12 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 566DM UT WOS:000275350300010 ER PT J AU Frye, JG Lindsey, RL Rondeau, G Porwollik, S Long, F McClelland, M Jackson, CR Englen, MD Meinersmann, RJ Berrang, ME Davis, JA Barrett, JB Turpin, JB Thitaram, SN Fedorka-Cray, PJ AF Frye, Jonathan G. Lindsey, Rebecca L. Rondeau, Gaelle Porwollik, Steffen Long, Fred McClelland, Michael Jackson, Charlene R. Englen, Mark D. Meinersmann, Richard J. Berrang, Mark E. Davis, Johnnie A. Barrett, John B. Turpin, Jennifer B. Thitaram, Sutawee N. Fedorka-Cray, Paula J. TI Development of a DNA Microarray to Detect Antimicrobial Resistance Genes Identified in the National Center for Biotechnology Information Database SO MICROBIAL DRUG RESISTANCE LA English DT Article ID COMPLETE GENOME SEQUENCE; OLIGONUCLEOTIDE MICROARRAY; ESCHERICHIA-COLI; MACROLIDE RESISTANCE; SALMONELLA-ENTERICA; MOLECULAR CHARACTERIZATION; BACTERIA; STRAINS; VIRULENCE; DESIGN AB To understand the mechanisms and epidemiology of antimicrobial resistance (AR), the genetic elements responsible must be identified. Due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. To achieve this, AR genes in the National Center for Biotechnology Information GenBank database were identified by their annotations and compiled into a nonredundant list of 775 genes. A DNA microarray was constructed of 70mer oligonucelotide probes designed to detect these genes encoding resistances to aminoglycosides, beta-lactams, chloramphenicols, glycopeptides, heavy metals, lincosamides, macrolides, metronidazoles, polyketides, quaternary ammonium compounds, streptogramins, sulfonamides, tetracyclines, and trimethoprims as well as resistance transfer genes. The microarray was validated with two fully sequenced control strains of Salmonella enterica: Typhimurium LT2 (sensitive) and Typhi CT18 (multidrug resistance [MDR]). All resistance genes encoded on the MDR plasmid, pHCM1, harbored by CT18 were detected in that strain, whereas no resistance genes were detected in LT2. The microarray was also tested with a variety of bacteria, including MDR Salmonella enterica serovars, Escherichia coli, Campylobacter spp., Enterococcus spp., methicillin-resistant Staphylococcus aureus, Listeria spp., and Clostridium difficile. The results presented here demonstrate that a microarray can be designed to detect virtually all AR genes found in the National Center for Biotechnology Information database, thus reducing the subsequent assays necessary to identify specific resistance gene alleles. C1 [Frye, Jonathan G.; Lindsey, Rebecca L.; Jackson, Charlene R.; Englen, Mark D.; Meinersmann, Richard J.; Berrang, Mark E.; Davis, Johnnie A.; Barrett, John B.; Turpin, Jennifer B.; Thitaram, Sutawee N.; Fedorka-Cray, Paula J.] ARS, USDA, Richard B Russell Res Ctr, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30605 USA. [Rondeau, Gaelle; Porwollik, Steffen; Long, Fred; McClelland, Michael] Sidney Kimmel Canc Ctr, San Diego, CA USA. RP Frye, JG (reprint author), ARS, USDA, Richard B Russell Res Ctr, Bacterial Epidemiol & Antimicrobial Resistance Re, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jonathan.frye@ars.usda.gov RI McClelland, Michael/A-8583-2011; Frye, Jonathan/I-6382-2013; OI Frye, Jonathan/0000-0002-8500-3395; McClelland, Michael/0000-0003-1788-9347 FU NIH [R01AI34829, R21AI057733] FX The authors thank Mike Asher, LaShanda Glenn, Russ Turpin, Jonathan Cudnik, Jovita Haro, Lari Hiott, and Takiyah Ball for technical assistance. M. M. was funded by NIH Grants R01AI34829 and R21AI057733 and by the generosity of Sidney Kimmel. The authors would also like to thank the NCBI helpdesk for assistance in searching the nonredundant sequence database. NR 46 TC 15 Z9 16 U1 1 U2 12 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD MAR PY 2010 VL 16 IS 1 BP 9 EP 19 DI 10.1089/mdr.2009.0082 PG 11 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA 569DF UT WOS:000275575800002 PM 19916789 ER PT J AU Rehfeldt, GE Jaquish, BC AF Rehfeldt, Gerald E. Jaquish, Barry C. TI Ecological impacts and management strategies for western larch in the face of climate-change SO MITIGATION AND ADAPTATION STRATEGIES FOR GLOBAL CHANGE LA English DT Article DE Bioclimate models; Mapped genetic variation; Seed zones; Seed transfer guidelines; Climate responses; Climatic niche ID LARIX-OCCIDENTALIS; GENETIC-VARIATION; BRITISH-COLUMBIA; EVOLUTIONARY RESPONSES; FUTURE CLIMATES; PINUS-CONTORTA; UNITED-STATES; TREE; POPULATIONS; GROWTH AB Approximately 185,000 forest inventory and ecological plots from both USA and Canada were used to predict the contemporary distribution of western larch (Larix occidentalis Nutt.) from climate variables. The random forests algorithm, using an 8-variable model, produced an overall error rate of about 2.9 %, nearly all of which consisted of predicting presence at locations where the species was absent. Genetic variation among 143 populations within western larch's natural distribution was predicted from multiple regression models using variables describing the climate of the seed source as predictors and response data from two separate genetic tests: 1) 15-year height at a field site in British Columbia, Canada, and, 2) two principal components of 8 variables describing growth, disease tolerance, and phenology of 6-year-old trees in a test in Idaho, USA. Presence and absence of the species and genetic variation within the species were projected into future climates provided by three General Circulation Models and two scenarios. Although the projections described pronounced impacts on the species and its populations, concurrence among the six projections pinpointed areas where the probability would be high that the future climate would be suitable for western larch. Concurrence among projections also was used to locate those sources of seed that should be best attuned genetically to future climates. The procedures outline a logical approach for developing management strategies for accommodating climate-change while taking into account the variability imposed by the differences among climatic estimates. C1 [Rehfeldt, Gerald E.] US Forest Serv, Rocky Mt Res Stn, USDA, Forestry Sci Lab, Moscow, ID 83843 USA. [Jaquish, Barry C.] Kalamalka Forestry Ctr, BC Minist Forests & Range, Res Branch, Vernon, BC V1B 2C7, Canada. RP Rehfeldt, GE (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, Forestry Sci Lab, 1221 S Main, Moscow, ID 83843 USA. EM jrehfeldt@gmail.com NR 60 TC 43 Z9 44 U1 0 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1381-2386 J9 MITIG ADAPT STRAT GL JI Mitig. Adapt. Strateg. Glob. Chang. PD MAR PY 2010 VL 15 IS 3 BP 283 EP 306 DI 10.1007/s11027-010-9217-2 PG 24 WC Environmental Sciences SC Environmental Sciences & Ecology GA 659GM UT WOS:000282555000005 ER PT J AU Yeh, HY Klesius, PH AF Yeh, Hung-Yueh Klesius, Phillip H. TI Characterization and tissue expression of channel catfish (Ictalurus punctatus Rafinesque, 1818) ubiquitin carboxyl-terminal hydrolase L5 (UCHL5) cDNA SO MOLECULAR BIOLOGY REPORTS LA English DT Article DE Channel catfish; Ictalurus punctatus; Ubiquitun carboxyl-terminal hydrolase L5; UCHL5; Ubiquitin ID DEUBIQUITINATING ENZYME; PROTEASOME SUBUNIT; MOLECULAR-CLONING; 26S PROTEASOMES; FISSION YEAST; GENE; UCH37; IDENTIFICATION; SPECIFICITY; SEQUENCES AB The ubiquitin-proteasome cycle is a complex, non-lysosomal biochemical process for intracellular protein degradation. This process involves many enzymes. One enzyme involved in this process is ubiquitin carboxyl-terminal hydrolase L5 (UCHL5), which deubiquitylates the polyubiquitin chain into ubiquitin. In this report, we isolated, sequenced, and characterized the channel catfish UCHL5 cDNA. The complete nucleic acid sequence of the channel catfish UCHL5 cDNA is comprised of 1,357 nucleotides, including an open reading frame, which appears to encode a putative peptide of 329 amino acid residues. The estimated molecular mass and pI of this peptide are 37.6 kDa and 4.84 at pH 7.0, respectively. The degree of conservation of the channel catfish UCHL5 amino acid sequence in comparison to other species ranged from 85% (vs. mouse) to 92% (vs. zebrafish and spotted green pufferfish). The channel catfish UCHL5 transcript was detected by RT-PCR in spleen, head kidney, liver, intestine, skin and gill, suggesting the UCHL5 transcript is constitutively expressed. This research provides important information for further elucidating UCHL5 in the channel catfish ubiquitin-proteasome pathway. C1 [Yeh, Hung-Yueh; Klesius, Phillip H.] ARS, Aquat Anim Hlth Res Unit, USDA, Auburn, AL 36832 USA. RP Yeh, HY (reprint author), ARS, Aquat Anim Hlth Res Unit, USDA, 990 Wire Rd, Auburn, AL 36832 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS [6420-32000-020-00D] FX We are grateful to Ms. Ifayet Johnson-Mayo and Mrs. Dorothy B. Moseley of the USDA ARS Aquatic Animal Health Research Unit in Auburn, AL for excellent technical support, and Dr. Brian E. Scheffler and his Bioinformatics Group at the USDA ARS MidSouth Genomics Laboratory in Stoneville, MS for sequencing and bioinformatics. We also thank Dr. Thomas L. Welker (USDA ARS Aquatic Animal Health Research Unit) and Dr. Dieter Steinhagen (University of Veterinary Medicine, Hannover, Germany) for critical comments on this manuscript. This study was supported by the USDA Agricultural Research Service CRIS project no. 6420-32000-020-00D. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 37 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0301-4851 J9 MOL BIOL REP JI Mol. Biol. Rep. PD MAR PY 2010 VL 37 IS 3 BP 1229 EP 1234 DI 10.1007/s11033-009-9493-7 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 551MA UT WOS:000274212500011 PM 19266312 ER PT J AU Jia, JZ Liu, CJ Wang, DW Chao, SM Wang, ML Qi, XQ AF Jia, Jizeng Liu, Chunji Wang, Daowen Chao, Shiaoman Wang, Mingli Qi, Xiaoquan TI Mike Gale and cereal genetics research in China OBITUARY SO MOLECULAR BREEDING LA English DT Biographical-Item C1 [Jia, Jizeng] CAAS, Inst Crop Sci, Beijing 100081, Peoples R China. [Liu, Chunji] CSIRO Plant Ind, Queensland Biosci Precent, Brisbane, Qld 4067, Australia. [Wang, Daowen] Chinese Acad Sci, Inst Genet & Dev, Beijing 100101, Peoples R China. [Chao, Shiaoman] ARS, USDA, Biosci Res Lab, Fargo, ND 58105 USA. [Wang, Mingli] ARS, USDA, Griffin, GA 30223 USA. [Qi, Xiaoquan] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. RP Jia, JZ (reprint author), CAAS, Inst Crop Sci, Beijing 100081, Peoples R China. EM jzjia@mail.caas.net.cn RI Liu, Chunji/B-5243-2009; Qi, Xiaoquan/G-1545-2010 OI Qi, Xiaoquan/0000-0001-7175-115X NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2010 VL 25 IS 3 BP 365 EP 367 DI 10.1007/s11032-010-9398-0 PG 3 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 559UK UT WOS:000274853300001 ER PT J AU Yan, JB Yang, XH Shah, T Sanchez-Villeda, H Li, JS Warburton, M Zhou, Y Crouch, JH Xu, YB AF Yan, Jianbing Yang, Xiaohong Shah, Trushar Sanchez-Villeda, Hector Li, Jiansheng Warburton, Marilyn Zhou, Yi Crouch, Jonathan H. Xu, Yunbi TI High-throughput SNP genotyping with the GoldenGate assay in maize SO MOLECULAR BREEDING LA English DT Article DE Single nucleotide polymorphism; Maize; Goldengate; High-throughput ID FRAGMENT-LENGTH-POLYMORPHISMS; MOLECULAR MARKERS; DIVERSITY; MICROSATELLITES; POPULATION; SELECTION; REPEATS; GENOME; GRAIN AB Single nucleotide polymorphisms (SNPs) are abundant and evenly distributed throughout the genomes of most plant species. They have become an ideal marker system for genetic research in many crops. Several high throughput platforms have been developed that allow rapid and simultaneous genotyping of up to a million SNP markers. In this study, a custom GoldenGate assay containing 1,536 SNPs was developed based on public SNP information for maize and used to genotype two recombinant inbred line (RIL) populations (Zong3 x 87-1, and B73 x By804) and a panel of 154 diverse inbred lines. Over 90% of the SNPs were successfully scored in the diversity panel and the two RIL populations, with a genotyping error rate of less than 2%. A total of 975 SNP markers detected polymorphism in at least one of the two mapping populations, with a polymorphic rate of 38.5% in Zong3 x 87-1 and 52.6% in B73 x By804. The polymorphic SNPs in B73 x By804 have been integrated with previously mapped simple sequence repeat markers to construct a high-density linkage map containing 662 markers with a total length of 1,673.7 cM and an average of 2.53 cM between two markers. The minor allelic frequency (MAF) was distributed evenly across 10 continued classes from 0.05 to 0.5, and about 16% of the SNP markers had a MAF below 10% in the diversity panel. Polymorphism rates for individual SNP markers in pair-wise comparisons of genotypes tested ranged from 0.3 to 63.8% with an average of 36.3%. Most SNPs used in this GoldenGate assay appear to be equally useful for diversity analysis, marker-trait association studies, and marker-aided breeding. C1 [Yan, Jianbing; Shah, Trushar; Sanchez-Villeda, Hector; Crouch, Jonathan H.; Xu, Yunbi] CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico. [Yan, Jianbing; Yang, Xiaohong; Li, Jiansheng; Zhou, Yi] China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China. [Warburton, Marilyn] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Yan, JB (reprint author), CIMMYT, Int Maize & Wheat Improvement Ctr, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. EM j.yan@cgiar.org; y.xu@cgiar.org FU National Hi-Tech Research and Development Program of China; Bill & Melinda Gates Foundation FX We highly appreciate the molecular and functional diversity team of the NSF Maize Genome Project for making available all the SNPs information used in this study. We thank Dr. Ortiz Rodomiro (CIMMYT) for his critical review this manuscript. This research was supported by the National Hi-Tech Research and Development Program of China and the Bill & Melinda Gates Foundation through the Drought Tolerant Maize for Africa project (http://dtma.cimmyt.org/). NR 24 TC 104 Z9 121 U1 1 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2010 VL 25 IS 3 BP 441 EP 451 DI 10.1007/s11032-009-9343-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 559UK UT WOS:000274853300008 ER PT J AU Barkley, NA Chamberlin, KDC Wang, ML Pittman, RN AF Barkley, Noelle A. Chamberlin, Kelly D. Chenault Wang, Ming Li Pittman, Roy N. TI Development of a real-time PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.) SO MOLECULAR BREEDING LA English DT Article DE Real-time PCR; Oleic acid (C18:1); Peanut (Arachis hypogaea L.); Gas chromatography; Indel; Microsomol-oleoyl PC desaturase ID HIGH-OLEATE TRAIT; GERMPLASM COLLECTION; MINI-CORE; REGISTRATION; DESATURASE; INHERITANCE; MUTATION; GENE; OIL AB Oleic acid, a monounsaturated, omega-9 fatty acid found in peanut (Arachis hypogaea L.) oil is an important seed quality trait because it provides increased shelf life, improved flavor, enhanced fatty acid composition, and has a beneficial effect on human health. Hence, a concentrated effort has been put forth on developing peanut cultivars that have high oleic acid (> 74%) and a low amount (< 10%) of linoleic acid, a polyunsaturated omega-6 fatty acid. A main bottleneck, however, in breeding research is fast selection of the trait(s) of interest. Therefore, in an effort to expedite breeding efforts, a real-time PCR genotyping assay was developed to rapidly identify the wild type and the mutant allele that are responsible for normal or high levels of oleic acid, respectively in peanut seeds. This test utilizes two TaqMan(A (R)) probes to detect the presence of an indel (insertion/deletion) in FAD2B and can be employed on DNA extracted from either seeds or leaves. The presence of the insertion (mutant allele) in fad2B causes a frameshift downstream in the coding sequence that ultimately alters the mRNA transcript level, and thus, decreases the activity of microsomal oleoyl-PC desaturase enzyme which converts oleic acid (C18:1) to linoleic acid (C18:2). Validation of the real-time assay was carried out by quantitatively evaluating the fatty acid composition by gas chromatography (GC). Overall, this real-time PCR assay facilitates the identification of progeny carrying the high oleic acid alleles, and thus, allows early elimination of undesirable non-high oleic acid lines in segregating populations. C1 [Barkley, Noelle A.; Wang, Ming Li; Pittman, Roy N.] ARS, USDA, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Chamberlin, Kelly D. Chenault] ARS, USDA, Wheat Peanut & Other Field Crops Res Unit, Western Stillwater, OK 74075 USA. RP Wang, ML (reprint author), ARS, USDA, Plant Genet Resources Conservat Unit, 1109 Expt St, Griffin, GA 30223 USA. EM MingLi.Wang@ars.usda.gov RI Barkley, Noelle/C-5815-2008 NR 23 TC 22 Z9 30 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2010 VL 25 IS 3 BP 541 EP 548 DI 10.1007/s11032-009-9338-z PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 559UK UT WOS:000274853300016 ER PT J AU Whittall, JB Syring, J Parks, M Buenrostro, J Dick, C Liston, A Cronn, R AF Whittall, J. B. Syring, J. Parks, M. Buenrostro, J. Dick, C. Liston, A. Cronn, R. TI Finding a (pine) needle in a haystack: chloroplast genome sequence divergence in rare and widespread pines SO MOLECULAR ECOLOGY LA English DT Article DE chloroplast genome; multiplex sequencing-by-synthesis; next-generation sequencing; Pinus ID SINGLE NUCLEOTIDE POLYMORPHISMS; MITOCHONDRIAL-DNA; PLANT-POPULATIONS; GLACIAL REFUGIA; PHYLOGENETIC-RELATIONSHIPS; GENETIC-STRUCTURE; SEED PLANTS; INHERITANCE; MICROSATELLITES; DIVERSITY AB Critical to conservation efforts and other investigations at low taxonomic levels, DNA sequence data offer important insights into the distinctiveness, biogeographic partitioning and evolutionary histories of species. The resolving power of DNA sequences is often limited by insufficient variability at the intraspecific level. This is particularly true of studies involving plant organelles, as the conservative mutation rate of chloroplasts and mitochondria makes it difficult to detect polymorphisms necessary to track genealogical relationships among individuals, populations and closely related taxa, through space and time. Massively parallel sequencing (MPS) makes it possible to acquire entire organelle genome sequences to identify cryptic variation that would be difficult to detect otherwise. We are using NIPS to evaluate intraspecific chloroplast-level divergence across biogeographic boundaries in narrowly endemic and widespread species of Pinus. We focus on one of the world's rarest pines - Torrey pine (Pinus torreyana) - due to its conservation interest and because it provides a marked contrast to more widespread pine species. Detailed analysis of nearly 90% (similar to 105 000 bp each) of these chloroplast genomes shows that mainland and island populations of Torrey pine differ at five sites in their plastome, with the differences fixed between populations. This is an exceptionally low level of divergence (1 polymorphism/similar to 21 kb), yet it is comparable to intraspecific divergence present in widespread pine species and species complexes. Population-level organelle genome sequencing offers new vistas into the timing and magnitude of divergence within species, and is certain to provide greater insight into pollen dispersal, migration patterns and evolutionary dynamics in plants. C1 [Cronn, R.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Whittall, J. B.; Buenrostro, J.; Dick, C.] Santa Clara Univ, Dept Biol, Santa Clara, CA 95053 USA. [Syring, J.] Linfield Coll, Dept Biol, Mcminnville, OR 97128 USA. [Parks, M.; Liston, A.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. RP Cronn, R (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM rcronn@fs.fed.us OI Cronn, Richard/0000-0001-5342-3494 FU NSF [0733078]; OSU College of Science Venture Fund [0629508]; USFS PNW Research FX We thank Bob Haller and Dieter Wilken for providing Torrey pine needles from the Santa Barbara Botanic Garden. Brian Knaus, Darren Smith, Stuphanie Kim, Tim Butler, Mariah Parker-DeFeniks, Ugi Daalkhaijav, Sarah Sundholm, Angela Rodriguez, David Gernandt, Rongkun Shen, Todd Mockler, Mark Dasenko, Scott Givan, Chris Sullivan, Ismael Grachico, Jon Laurent and John Reeves provided critical assistance. Support provided by the Santa Barbara Botanic Garden and NSF IPY No. 0733078 to JBkV, the OSU College of Science Venture Fund to AL, NSF ATOL No. 0629508 to AL and RC, and USFS PNW Research Station. NR 65 TC 38 Z9 41 U1 4 U2 29 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD MAR PY 2010 VL 19 SU 1 BP 100 EP 114 DI 10.1111/j.1365-294X.2009.04474.x PG 15 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 570BD UT WOS:000275645700010 PM 20331774 ER PT J AU An, JH Bechet, A Berggren, A Brown, SK Bruford, MW Cai, QG Cassel-Lundhagen, A Cezilly, F Chen, SL Cheng, W Choi, SK Ding, XY Fan, Y Feldheim, KA Feng, ZY Friesen, VL Gaillard, M Galaraza, JA Gallo, L Ganeshaiah, KN Geraci, J Gibbons, JG Grant, WS Grauvogel, Z Gustafsson, S Guyon, JR Han, L Heath, DD Hemmila, S Hogan, JD Hou, BW Jakse, J Javornik, B Kanuch, P Kim, KK Kim, KS Kim, SG Kim, SI Kim, WJ Kim, YK Klich, MA Kreiser, BR Kwan, YS Lam, AW Lasater, K Lascoux, M Lee, H Lee, YS Li, DL Li, SJ Li, WY Liao, XL Liber, Z Lin, L Liu, SY Luo, XH Ma, YH Ma, YJ Marchelli, P Min, MS Moccia, MD Mohana, KP Moore, M Morris-Pocock, JA Park, HC Pfunder, M Radosavljevic, I Ravikanth, G Roderick, GK Rokas, A Sacks, BN Saski, CA Satovic, Z Schoville, SD Sebastiani, F Sha, ZX Shin, EH Soliani, C Sreejayan, N Sun, ZX Tao, Y Taylor, SA Templin, WD Shaanker, RU Vasudeva, R Vendramin, GG Walter, RP Wang, GZ Wang, KJ Wang, YQ Wattier, RA Wei, FW Widmer, A Woltmann, S Won, YJ Wu, J Xie, ML Xu, GB Xu, XJ Ye, HH Zhan, XJ Zhang, F Zhong, J AF An, Junghwa Bechet, Arnaud Berggren, Asa Brown, Sarah K. Bruford, Michael W. Cai, Qingui Cassel-Lundhagen, Anna Cezilly, Frank Chen, Song-Lin Cheng, Wei Choi, Sung-Kyoung Ding, X. Y. Fan, Yong Feldheim, Kevin A. Feng, Z. Y. Friesen, Vicki L. Gaillard, Maria Galaraza, Juan A. Gallo, Leonardo Ganeshaiah, K. N. Geraci, Julia Gibbons, John G. Grant, William S. Grauvogel, Zac Gustafsson, S. Guyon, Jeffrey R. Han, L. Heath, Daniel D. Hemmilae, S. Hogan, J. Derek Hou, B. W. Jakse, Jernej Javornik, Branka Kanuch, Peter Kim, Kyung-Kil Kim, Kyung-Seok Kim, Sang-Gyu Kim, Sang-In Kim, Woo-Jin Kim, Yi-Kyung Klich, Maren A. Kreiser, Brian R. Kwan, Ye-Seul Lam, Athena W. Lasater, Kelly Lascoux, M. Lee, Hang Lee, Yun-Sun Li, D. L. Li, Shao-Jing Li, W. Y. Liao, Xiaolin Liber, Zlatko Lin, Lin Liu, Shaoying Luo, Xin-Hui Ma, Y. H. Ma, Yajun Marchelli, Paula Min, Mi-Sook Moccia, Maria Domenica Mohana, Kumara P. Moore, Marcelle Morris-Pocock, James A. Park, Han-Chan Pfunder, Monika Radosavljevic, Ivan Ravikanth, G. Roderick, George K. Rokas, Antonis Sacks, Benjamin N. Saski, Christopher A. Satovic, Zlatko Schoville, Sean D. Sebastiani, Federico Sha, Zhen-Xia Shin, Eun-Ha Soliani, Carolina Sreejayan, N. Sun, Zhengxin Tao, Yong Taylor, Scott A. Templin, William D. Shaanker, R. Uma Vasudeva, R. Vendramin, Giovanni G. Walter, Ryan P. Wang, Gui-Zhong Wang, Ke-Jian Wang, Y. Q. Wattier, Remi A. Wei, Fuwen Widmer, Alex Woltmann, Stefan Won, Yong-Jin Wu, Jing Xie, M. L. Xu, Genbo Xu, Xiao-Jun Ye, Hai-Hui Zhan, Xiangjiang Zhang, F. Zhong, J. CA Mol Ecology Resources Primer Dev C TI Permanent Genetic Resources added to Molecular Ecology Resources Database 1 October 2009-30 November 2009 SO MOLECULAR ECOLOGY RESOURCES LA English DT Article AB This article documents the addition of 411 microsatellite marker loci and 15 pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Acanthopagrus schlegeli, Anopheles lesteri, Aspergillus clavatus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus oryzae, Aspergillus terreus, Branchiostoma japonicum, Branchiostoma belcheri, Colias behrii, Coryphopterus personatus, Cynogolssus semilaevis, Cynoglossus semilaevis, Dendrobium officinale, Dendrobium officinale, Dysoxylum malabaricum, Metrioptera roeselii, Myrmeciza exsul, Ochotona thibetana, Neosartorya fischeri, Nothofagus pumilio, Onychodactylus fischeri, Phoenicopterus roseus, Salvia officinalis L., Scylla paramamosain, Silene latifo, Sula sula, and Vulpes vulpes. These loci were cross-tested on the following species: Aspergillus giganteus, Colias pelidne, Colias interior, Colias meadii, Colias eurytheme, Coryphopterus lipernes, Coryphopterus glaucofrenum, Coryphopterus eidolon, Gnatholepis thompsoni, Elacatinus evelynae, Dendrobium loddigesii Dendrobium devonianum, Dysoxylum binectariferum, Nothofagus antarctica, Nothofagus dombeyii, Nothofagus nervosa, Nothofagus obliqua, Sula nebouxii, and Sula variegata. This article also documents the addition of 39 sequencing primer pairs and 15 allele specific primers or probes for Paralithodes camtschaticus. C1 [An, Junghwa; Choi, Sung-Kyoung; Kim, Kyung-Seok; Lasater, Kelly; Lee, Hang; Lee, Yun-Sun; Min, Mi-Sook; Park, Han-Chan] Seoul Natl Univ, Coll Vet Med, Program Vet Sci BK21, Seoul 151742, South Korea. [Bechet, Arnaud] Le Sambuc, La Tour Valat, F-13200 Arles, France. [Berggren, Asa; Cassel-Lundhagen, Anna; Kanuch, Peter] Swedish Univ Agr Sci, Dept Ecol, SE-75007 Uppsala, Sweden. [Brown, Sarah K.; Sacks, Benjamin N.] Univ Calif Davis, Canid Divers & Conservat Lab, Ctr Vet Genet, Davis, CA 95616 USA. [Bruford, Michael W.; Zhan, Xiangjiang] Cardiff Univ, Cardiff Sch Biosci, Biodivers & Ecol Proc Grp, Cardiff CF10 3AX, Wales. [Cai, Qingui] Caopo Nat Reserves, Wenchuan Cty 623000, Sichuan, Peoples R China. [Pfunder, Monika] Ecogenics GmbH, CH-8952 Zurich, Switzerland. [Cezilly, Frank; Gaillard, Maria; Geraci, Julia; Wattier, Remi A.] Univ Bourgogne, Equipe Ecol Evolut, CNRS, UMR Biogeosci 5561, F-21000 Dijon, France. [Chen, Song-Lin; Liao, Xiaolin; Luo, Xin-Hui; Sacks, Benjamin N.; Sha, Zhen-Xia; Xu, Genbo] Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China. [Cheng, Wei; Grant, William S.; Grauvogel, Zac; Templin, William D.] Alaska Dept Fish & Game, Anchorage, AK 99518 USA. [Ding, X. Y.; Feng, Z. Y.; Han, L.; Hou, B. W.; Ma, Y. H.; Xie, M. L.; Zhang, F.] Nanjing Normal Univ, Coll Life Sci, Jiangsu Provincal Key Lab Biodivers & Biotechnol, Nanjing 210097, Peoples R China. [Fan, Yong; Lin, Lin; Ma, Yajun; Wu, Jing] Second Mil Med Univ, Dept Pathogen Biol, Shanghai 200433, Peoples R China. [Fan, Yong; Lin, Lin; Ma, Yajun; Wu, Jing] Second Mil Med Univ, State Key Lab Med Immunol, Shanghai 200433, Peoples R China. [Feldheim, Kevin A.] Field Museum, Pritzker Lab Mol Systemat & Evolut, Chicago, IL 60605 USA. [Friesen, Vicki L.; Morris-Pocock, James A.; Sun, Zhengxin; Taylor, Scott A.] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada. [Galaraza, Juan A.] CSIC, Estac Biol Donana, Seville 41092, Spain. [Gallo, Leonardo; Marchelli, Paula; Soliani, Carolina] INTA EEA Bariloche, Unidad Genet Ecol & Mejoramiento Forestal, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Ganeshaiah, K. N.; Mohana, Kumara P.; Ravikanth, G.; Sreejayan, N.; Shaanker, R. Uma] Univ Agr Sci, Sch Ecol & Conservat, Bangalore 560065, Karnataka, India. [Ganeshaiah, K. N.; Ravikanth, G.; Shaanker, R. Uma] Ashoka Trust Res Ecol & Environm, Bangalore 560064, Karnataka, India. [Ganeshaiah, K. N.] Univ Agr Sci, Dept Forestry & Environm Sci, Bangalore 60065, Karnataka, India. [Gibbons, John G.; Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Gustafsson, S.; Hemmilae, S.; Lascoux, M.] Uppsala Univ, EBC, Program Evolutionary Funct Genom, Uppsala, Sweden. [Guyon, Jeffrey R.] NOAA, Auke Bay Labs, Alaska Fisheries Sci Ctr, NMFS, Juneau, AK 99801 USA. [Heath, Daniel D.; Hogan, J. Derek; Walter, Ryan P.] Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON N9B 3P4, Canada. [Jakse, Jernej; Javornik, Branka] Univ Ljubljana, Biotech Fac, Dept Agron, Ctr Plant Biotechnol & Breeding, Ljubljana 1000, Slovenia. [Kim, Kyung-Kil; Kim, Sang-Gyu; Kim, Woo-Jin; Kim, Yi-Kyung; Shin, Eun-Ha] Natl Fisheries Res & Dev Inst, Biotechnol Res Div, Pusan 619705, South Korea. [Kim, Sang-In; Lee, Hang; Min, Mi-Sook] Seoul Natl Univ, Conservat Genome Resource Bank Korean Wildlife, Res Inst Vet Sci, Seoul 151742, South Korea. [Klich, Maren A.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Kreiser, Brian R.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. [Kwan, Ye-Seul; Won, Yong-Jin] Ewha Womans Univ, Div EcoSci, Seoul, South Korea. [Lam, Athena W.; Roderick, George K.; Schoville, Sean D.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Li, D. L.; Li, W. Y.; Wang, Y. Q.; Zhong, J.] Xiamen Univ, Sch Life Sci, Key Lab, Minist Educ Coastal & Wetland Ecosyst, Xiamen 361005, Peoples R China. [Li, Shao-Jing; Tao, Yong; Wang, Gui-Zhong; Wang, Ke-Jian; Xu, Xiao-Jun; Ye, Hai-Hui] Xiamen Univ, Coll Oceanog & Environm Sci, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China. [Liber, Zlatko; Radosavljevic, Ivan] Univ Zagreb, Fac Sci, Dept Bot, Div Biol, HR-10000 Zagreb, Croatia. [Liu, Shaoying] Sichuan Acad Forestry, Chengdu 610066, Sichuan, Peoples R China. [Luo, Xin-Hui] Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China. [Marchelli, Paula; Soliani, Carolina] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Moccia, Maria Domenica; Widmer, Alex] ETH, IBZ, CH-8092 Zurich, Switzerland. [Mohana, Kumara P.; Shaanker, R. Uma] Univ Agr Sci, Dept Crop Physiol, Bangalore 560065, Karnataka, India. [Sacks, Benjamin N.] Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA. [Moore, Marcelle; Sacks, Benjamin N.] Calif State Univ Sacramento, Dept Biol Sci, Sacramento, CA 95819 USA. [Saski, Christopher A.] Clemson Univ, Genom Inst, Clemson, SC 29634 USA. [Satovic, Zlatko] Univ Zagreb, Fac Agr, Dept Seed Sci & Technol, Zagreb 10000, Croatia. [Sebastiani, Federico] Lab Genexpress, Dipartimento Biotecnol Agr, I-50019 Florence, Italy. [Vasudeva, R.] Univ Agr Sci, Dept Forest Biol & Tree Improvement, Coll Forestry, Sirsi, India. [Vendramin, Giovanni G.] CNR, Ist Genet Vegetale, I-50019 Florence, Italy. [Wei, Fuwen; Zhan, Xiangjiang] Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China. [Woltmann, Stefan] Tulane Univ, Dept Ecol & Evolutionary Biol, New Orleans, LA 70118 USA. [Xu, Genbo] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China. EM editorial.office@molecolres.com RI Hogan, J. Derek/C-2730-2008; Bruford, Michael/D-3750-2009; Widmer, Alex/B-6841-2009; Lee, Hang/A-7516-2011; Wang, YQ/G-4674-2010; Ye, HH/G-3997-2010; Lundhagen, Anna/B-9074-2011; Schoville, Sean/J-8061-2012; Wang, GZ/G-4644-2010; Wang, KJ/G-4609-2010; Kanuch, Peter/A-5416-2011; Rokas, Antonis/A-9775-2008; Vendramin, Giovanni G/K-9731-2014; CSIC, EBD Donana/C-4157-2011; Xu, Elvis /R-5543-2016; OI Bruford, Michael/0000-0001-6357-6080; Widmer, Alex/0000-0001-8253-5137; Lee, Hang/0000-0003-0264-6289; Lundhagen, Anna/0000-0003-4893-8527; Rokas, Antonis/0000-0002-7248-6551; Vendramin, Giovanni G/0000-0001-9921-7872; CSIC, EBD Donana/0000-0003-4318-6602; Jakse, Jernej/0000-0002-8907-1627; Satovic, Zlatko/0000-0002-9848-1601; SEBASTIANI, FEDERICO/0000-0003-4676-7381; Lascoux, Martin/0000-0003-1699-9042; Kanuch, Peter/0000-0003-2001-4541 NR 0 TC 28 Z9 29 U1 2 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAR PY 2010 VL 10 IS 2 BP 404 EP 408 DI 10.1111/j.1755-0998.2009.02827.x PG 5 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 552WQ UT WOS:000274325400021 ER PT J AU Wang, TTY Schoene, NW Kim, YS Mizuno, CS Rimando, AM AF Wang, Thomas T. Y. Schoene, Norberta W. Kim, Young S. Mizuno, Cassia S. Rimando, Agnes M. TI Differential effects of resveratrol and its naturally occurring methylether analogs on cell cycle and apoptosis in human androgen-responsive LNCaP cancer cells SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Androgen; Cancer prevention; Estrogen; Phytochemicals; Stilbenes ID PROSTATE-CANCER; IN-VIVO; EXPRESSION; RECEPTOR; PROGRESSION; STILBENES; ARREST; GENES; LINES; P21(WAF1/CIP1) AB Stilbenes are phytoalexins that become activated when plants are stressed. These compounds exist in foods and are widely consumed. Resveratrol is a grape-derived stilbene, which possesses a wide range of health-promoting activities, including anticancer properties. Several other stilbenes structurally similar to resveratrol are also available in food, but their biological activities remain largely unknown. In this study, we compared the effects of resveratrol and its natural derivatives pterostilbene, trans-resveratrol trimethylether, trans-pinostilbene and trans-desoxyrhapontigenin on androgen-responsive human prostate cancer LNCaP cells. We found that these compounds exert differential effects on LNCaP cell growth, cell cycle and apoptosis. Trans-resveratrol trimethylether appeared to be the most potent compound among the stilbenes tested. Treatment of LNCaP cells with trans-resveratrol trimethylether resulted in G2/M blockage while other compounds, including resveratrol, induced G1/S arrest. Moreover, different from other compounds, trans-resveratrol trimethylether induced apoptosis. At the molecular level, the effects of these compounds on cell cycle correlated with induction of the cyclin-dependent kinase inhibitor 1A and B mRNA levels. Additionally, these compounds also inhibited both androgen- as well as estrogen-mediated pathways. These results provide mechanistic information on how resveratrol and its methylether analogs may act to contribute to potential antiprostate cancer activity. C1 [Wang, Thomas T. Y.; Schoene, Norberta W.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Kim, Young S.] Natl Canc Inst, Nutrit Sci Res Grp, Canc Prevent Div, NIH, Bethesda, MD USA. [Mizuno, Cassia S.; Rimando, Agnes M.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS USA. RP Wang, TTY (reprint author), ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, 10300 Baltimore Ave,Bldg 307C,Room 132, Beltsville, MD 20705 USA. EM tom.wang@ars.usda.gov FU US appropriated funds [1235-51530-052-00, 6408-41000-006-00]; National Cancer Institute FX This work was supported by US appropriated funds to USDA project numbers 1235-51530-052-00 (TTYWN. WS), 6408-41000-006-00 (CSM, AMR) and the National Cancer Institute (YSK). NR 47 TC 52 Z9 53 U1 1 U2 8 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1613-4125 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD MAR PY 2010 VL 54 IS 3 BP 335 EP 344 DI 10.1002/mnfr.200900143 PG 10 WC Food Science & Technology SC Food Science & Technology GA 575JS UT WOS:000276063900003 PM 20077416 ER PT J AU Winkler, IS Rung, A Scheffer, SJ AF Winkler, Isaac S. Rung, Alessandra Scheffer, Sonja J. TI Hennig's orphans revisited: Testing morphological hypotheses in the "Opomyzoidea" (Diptera: Schizophora) SO MOLECULAR PHYLOGENETICS AND EVOLUTION LA English DT Article DE Acalyptrate; Phylogeny; Higher classification; Rapid radiation ID ACALYPTRATE FLIES DIPTERA; DNA-SEQUENCE DATA; BAYESIAN PHYLOGENETIC INFERENCE; LONG-BRANCH ATTRACTION; PERISCELIDIDAE DIPTERA; MOLECULAR PHYLOGENY; MUSCOIDEA DIPTERA; ANIMAL PHYLOGENY; TREE SELECTION; NUCLEAR GENES AB The acalyptrate fly superfamily Oponiyzoidea, as currently recognized, is a poorly-known group of 14 families. The composition of this group and relationships among included families have been controversial. Furthermore, the delimitation of two opomyzoid families, Aulacigastridae and Periscelididae, has been unstable with respect to placement of the genera Stenomicra, Cyamops, and Planinasus. To test the monophyly of Opomyzoidea, previously proposed relationships between families, and the position of the three problematic genera, we sequenced over 3300 bp of nucleotide sequence data from the 28S ribosomal DNA and CAD (rudimentary) genes from 29 taxa representing all opomyzoid families, as well as 13 outgroup taxa. Relationships recovered differed between analyses, and only branches supporting well-established monophyletic families were recovered with high support, with a few exceptions. Opomyzoidea and its included subgroup, Asteioinea, were found to be non-monophyletic. Stenormicra, Cyamops, and Planinasus group consistently with Aulacigistridae, contrary to recent classifications. Xenasteiidae and Australimyzidae, two small, monogeneric families placed in separate superfamilies, were strongly supported as sister groups. (C) 2009 Elsevier Inc. All rights reserved. C1 [Winkler, Isaac S.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Rung, Alessandra] Calif Dept Food & Agr, Plant Pest Diagnosit Branch, Sacramento, CA 95832 USA. [Rung, Alessandra; Scheffer, Sonja J.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD 20705 USA. [Winkler, Isaac S.; Rung, Alessandra; Scheffer, Sonja J.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. RP Winkler, IS (reprint author), N Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. EM iswinkle@ncsu.edu OI Winkler, Isaac/0000-0003-2453-021X NR 114 TC 11 Z9 13 U1 1 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1055-7903 EI 1095-9513 J9 MOL PHYLOGENET EVOL JI Mol. Phylogenet. Evol. PD MAR PY 2010 VL 54 IS 3 BP 746 EP 762 DI 10.1016/j.ympev.2009.12.016 PG 17 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 563YM UT WOS:000275176500007 PM 20040375 ER PT J AU Liu, JQ Allan, DL Vance, CP AF Liu, Jun-Qi Allan, Deborah L. Vance, Carroll P. TI Systemic Signaling and Local Sensing of Phosphate in Common Bean: Cross-Talk between Photosynthate and MicroRNA399 SO MOLECULAR PLANT LA English DT Article DE Roots; nutrient stress; photosynthate; signaling ID PHOSPHORUS STARVATION; ARABIDOPSIS-THALIANA; ROOT DEVELOPMENT; GENE-EXPRESSION; PLANT-RESPONSES; TRANSPORT; TOMATO; PHLOEM; PHO2; DEPRIVATION AB Shoot-to-root communication is crucial for plant acclimation to phosphorus (P)-deficiency. Both sugars and miRNAs have been implicated as potential signal molecules transported through phloem from shoot to root for the regulation of gene expression and Pi uptake in the root. By studying the expression patterns of both a serine/threonine phosphatase gene (PvHAD1) and microRNA399 (miR399) in common bean (Phaseolus vulgaris L.), we provide evidence for the interaction between light, phloem transport, and miR399 in the systemic regulation of gene expression under P-deficiency. Especially, miR399 expression in both the shoot and the root requires photosynthetic carbon assimilation during the onset of P-deficiency. In contrast to systemic signaling, local sensing was the primary causal factor for rapid down-regulation of PvHAD1 by Pi prior to the reduction of miR399 level in P-deficient roots. Furthermore, this initial response to Pi in P-deficient root was also mimicked by the Pi analog, phosphonate (Phi). Our current findings suggest that plants have developed a highly coordinated dual regulatory pathway, namely long-distance signaling of P-deficiency from shoot to root versus local sensing of Pi in the root. C1 [Liu, Jun-Qi; Vance, Carroll P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Allan, Deborah L.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Vance, Carroll P.] Agr Res Serv, USDA, St Paul, MN 55108 USA. RP Liu, JQ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM liuxx162@tc.umn.edu FU United States Department of Agriculture; National Research Initiative; CSREES [2005-35100-16002]; USDA Agricultural Research Service CRIS [3640-21000-024-00D]; University of Minnesota [58-3640-9-750] FX This research was funded in part by United States Department of Agriculture, National Research Initiative, CSREES Grant Number 2005-35100-16002; USDA Agricultural Research Service CRIS Number 3640-21000-024-00D; and University of Minnesota, Specific Cooperative Agreement Number 58-3640-9-750. NR 34 TC 47 Z9 51 U1 0 U2 17 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1674-2052 J9 MOL PLANT JI Mol. Plant. PD MAR PY 2010 VL 3 IS 2 BP 428 EP 437 DI 10.1093/mp/ssq008 PG 10 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 585RA UT WOS:000276844600014 PM 20147371 ER PT J AU Georgianna, DR Fedorova, ND Burroughs, JL Dolezal, AL Bok, JW Horowitz-Brown, S Woloshuk, CP Yu, JJ Keller, NP Payne, GA AF Georgianna, D. Ryan Fedorova, Natalie D. Burroughs, James L. Dolezal, Andrea L. Bok, Jin Woo Horowitz-Brown, Sigal Woloshuk, Charles P. Yu, Jiujiang Keller, Nancy P. Payne, Gary A. TI Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavus secondary metabolism gene clusters SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID CYCLOPIAZONIC ACID PRODUCTION; CONIDIAL PIGMENT BIOSYNTHESIS; PROBE LEVEL DATA; FILAMENTOUS FUNGI; CHEMICAL DIVERSITY; NEUROSPORA-CRASSA; OXIDATIVE STRESS; FUMIGATUS; PARASITICUS; VIRULENCE AB Species of Aspergillus produce a diverse array of secondary metabolites, and recent genomic analysis has predicted that these species have the capacity to synthesize many more compounds. It has been possible to infer the presence of 55 gene clusters associated with secondary metabolism in Aspergillus flavus; however, only three metabolic pathways-aflatoxin, cyclopiazonic acid (CPA) and aflatrem-have been assigned to these clusters. To gain an insight into the regulation of and to infer the ecological significance of the 55 secondary metabolite gene clusters predicted in A. flavus, we examined their expression over 28 diverse conditions. Variables included culture medium and temperature, fungal development, colonization of developing maize seeds and misexpression of laeA, a global regulator of secondary metabolism. Hierarchical clustering analysis of expression profiles allowed us to categorize the gene clusters into four distinct clades. Gene clusters for the production of aflatoxins, CPA and seven other unknown compound(s) were identified as belonging to one clade. To further explore the relationships found by gene expression analysis, aflatoxin and CPA production were quantified under five different cell culture environments known to be conducive or nonconducive for aflatoxin biosynthesis and during the colonization of developing maize seeds. Results from these studies showed that secondary metabolism gene clusters have distinctive gene expression profiles. Aflatoxin and CPA were found to have unique regulation, but are sufficiently similar that they would be expected to co-occur in substrates colonized with A. flavus. C1 [Georgianna, D. Ryan; Burroughs, James L.; Dolezal, Andrea L.; Payne, Gary A.] N Carolina State Univ, Dept Plant Pathol, Ctr Integrated Fungal Res, Raleigh, NC 27695 USA. [Fedorova, Natalie D.] J Craig Venter Inst, Rockville, MD 20850 USA. [Bok, Jin Woo; Horowitz-Brown, Sigal; Keller, Nancy P.] Univ Wisconsin, Dept Plant Pathol, Dept Med Microbiol & Immunol, Madison, WI 53706 USA. [Woloshuk, Charles P.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Yu, Jiujiang] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Payne, GA (reprint author), N Carolina State Univ, Dept Plant Pathol, Ctr Integrated Fungal Res, Raleigh, NC 27695 USA. EM gary_payne@ncsu.edu RI Bok, Jin Woo/F-7140-2010; Abrams, Natalie/F-4845-2011 OI Abrams, Natalie/0000-0001-9698-2819 FU US Department of Agriculture [2006-35604-16666]; National Institutes of Health/North Carolina State University Molecular Biotechnology Training Program FX Thanks are due to the US Department of Agriculture CSRS grant 2006-35604-16666 and the National Institutes of Health/North Carolina State University Molecular Biotechnology Training Program for their generous support of this research. NR 66 TC 65 Z9 70 U1 2 U2 24 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1464-6722 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD MAR PY 2010 VL 11 IS 2 BP 213 EP 226 DI 10.1111/j.1364-3703.2009.00594.x PG 14 WC Plant Sciences SC Plant Sciences GA 547PW UT WOS:000273902500005 PM 20447271 ER PT J AU Naderpour, M Lund, OS Larsen, R Johansen, E AF Naderpour, Masoud Lund, Ole Sogaard Larsen, Richard Johansen, Elisabeth TI Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID COMMON-MOSAIC-VIRUS; TRANSLATION INITIATION-FACTORS; YEAST 2-HYBRID SYSTEM; VENOUS-MOTTLE-VIRUS; FACTOR ISO 4E; ARABIDOPSIS-THALIANA; RECESSIVE RESISTANCE; PISUM-SATIVUM; I-GENE; PROTEIN AB Eukaryotic translation initiation factors (eIFs) play a central role in potyviral infection. Accordingly, mutations in the gene encoding eIF4E have been identified as a source of recessive resistance in several plant species. In common bean, Phaseolus vulgaris, four recessive genes, bc-1, bc-2, bc-3 and bc-u, have been proposed to control resistance to the potyviruses Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus. In order to identify molecular entities for these genes, we cloned and sequenced P. vulgaris homologues of genes encoding the eIF proteins eIF4E, eIF(iso)4E and nCBP. Bean genotypes reported to carry bc-3 resistance were found specifically to carry non-silent mutations at codons 53, 65, 76 and 111 in eIF4E. This set of mutations closely resembled a pattern of eIF4E mutations determining potyvirus resistance in other plant species. The segregation of BCMV resistance and eIF4E genotype was subsequently analysed in an F(2) population derived from the P. vulgaris all-susceptible genotype and a genotype carrying bc-3. F(2) plants homozygous for the eIF4E mutant allele were found to display at least the same level of resistance to BCMV as the parental resistant genotype. At 6 weeks after inoculation, all F(2) plants found to be BCMV negative by enzyme-linked immunosorbent assay were found to be homozygous for the mutant eIF4E allele. In F(3) plants homozygous for the mutated allele, virus resistance was subsequently found to be stably maintained. In conclusion, allelic eIF4E appears to be associated with a major component of potyvirus resistance present in bc-3 genotypes of bean. C1 [Naderpour, Masoud; Johansen, Elisabeth] Univ Aarhus, Fac Agr Sci, Dept Genet & Biotechnol, DK-1871 Frederiksberg C, Denmark. [Naderpour, Masoud] Seed & Plant Certificat Res Inst, Karaj 315353383, Iran. [Lund, Ole Sogaard] Univ Copenhagen, Fac Life Sci, Dept Plant Biol, DK-1871 Frederiksberg C, Denmark. [Larsen, Richard] ARS, Vegetable & Forage Crops Res Lab, USDA, Prosser, WA 99350 USA. RP Naderpour, M (reprint author), Univ Aarhus, Fac Agr Sci, Dept Genet & Biotechnol, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. EM m.naderpour@dias.kvl.dk RI Johansen, Ida/F-7792-2014; Lund, Ole /G-9346-2014 OI Johansen, Ida/0000-0001-8660-2018; Lund, Ole /0000-0001-9591-3897 FU Iranian Ministry of Science, Research and Technology; Ministry of Agriculture; Danish Agricultural and Veterinary Research Council [53-00-0330] FX We are grateful to CIAT for the seeds of the bean genotypes. This project was supported financially by a PhD grant to MN from the Iranian Ministry of Science, Research and Technology and Ministry of Agriculture, and by the Danish Agricultural and Veterinary Research Council grant no. 53-00-0330 to OSL. NR 35 TC 30 Z9 31 U1 0 U2 10 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1464-6722 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD MAR PY 2010 VL 11 IS 2 BP 255 EP 263 DI 10.1111/j.1364-3703.2009.00602.x PG 9 WC Plant Sciences SC Plant Sciences GA 547PW UT WOS:000273902500008 PM 20447274 ER PT J AU Wang, Y Pei, YF O'Neill, NR Zhang, XG AF Wang, Yong Pei, Yun-Fei O'Neill, Nichole R. Zhang, Xiu-Guo TI Ulocladium cantlous sp nov isolated from northwestern China: its morphology and molecular phylogenetic position SO MYCOLOGIA LA English DT Article DE hyphomycetes; new species; systematics ID DEHYDROGENASE GENE-SEQUENCES; ALTERNARIA THEMES; LEAF-SPOT; STEMPHYLIUM; BOTRYTIS; ALT-A-1; IDENTIFICATION; BRASSICICOLA; CHARTARUM; EVOLUTION AB A new species of Ulocladium was isolated from diseased leaves from two Cucumis sp. growing in Sinkiang and Gansu provinces of China. Conidia were isolated from necrotic leaves and used to establish single-spore pure cultures. Conida were harvested from cultures 7 d after incubation for morphological comparisons. The morphology of this species resembles that of U. botrytis and U. consortiale. However it is distinguished from these two species by the sizes of obovoid to broadly ellipsoidal conidia and longer conidiophores. A taxonomic description of U. cantlous, comparison with related species in this genus, and a species phylogeny based on the partial nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene and the Alternaira allernata major allergen (Alt a 1) gene are provided. C1 [Wang, Yong; Pei, Yun-Fei; Zhang, Xiu-Guo] Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China. [O'Neill, Nichole R.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Zhang, XG (reprint author), Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China. EM zhxg@sdau.edu.cn FU National Natural Science Foundation of China [30570006] FX This work was supported by the National Natural Science Foundation of China (Grant 30570006). NR 32 TC 2 Z9 2 U1 0 U2 5 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2010 VL 102 IS 2 BP 374 EP 383 DI 10.3852/09-093 PG 10 WC Mycology SC Mycology GA 571HW UT WOS:000275743900011 PM 20361504 ER PT J AU Hirooka, Y Kobayashi, T Ono, T Rossman, AY Chaverri, P AF Hirooka, Yuuri Kobayashi, Takao Ono, Tsuyoshi Rossman, Amy Y. Chaverri, Priscila TI Verrucostoma, a new genus in the Bionectriaceae from the Bonin Islands, Japan SO MYCOLOGIA LA English DT Article DE Ascomycota; Freycinetia; Hypocreales; molecular systematics; Pandanaceae ID BAYESIAN-INFERENCE; RIBOSOMAL DNA; PHYLOGENY; HYPOCREALES; NECTRIA; LIKELIHOOD; ALGORITHM; EVOLUTION; SEQUENCES; ANAMORPH AB Verrucostoma freycinetiae gen. et sp. nov. is described and illustrated from specimens on dead leaves of Freycinetia boninensis (Pandanaceae) collected in Hahajima, Bonin (Ogasawara) Islands, Japan. The genus is characterized by pale orange perithecia with protuberances around the perithecial apex, no color change in 3% potassium hydroxide and lactic acid, unitunicate asci, spinulose ascospores and an Acremonium-like anamorph. Morphological characters were compared with other genera in the Bionectriaceae and Nectriaceae (Hypocreales). Verrucostoma is morphologically similar to Bionectria (Bionectriaceae) from which it differs in the formation of conspicuous protuberances around the perithecial apex and the Acremonium-like anamorph Moreover molecular analyses of Verrucostoma and other members of the Bionectriaceae and Nectriaceae based on alpha-actin, large subunit nuclear ribosomal DNA and RNA polymerase II subunit 1 sequences Support, the Conclusions based oil morphological data. Our results confirm that V. freycinetiae is distinct from other genera among the Nectria-like fungi and wrepresents a new genus belonging to the Bionectriaceae. C1 [Hirooka, Yuuri; Rossman, Amy Y.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Kobayashi, Takao] Tokyo Univ Agr, Dept Int Agr Dev, Setagaya Ku, Tokyo 1568502, Japan. [Ono, Tsuyoshi] Tokyo Metropolitan Agr & Forestry Res Ctr, Tokyo 1900013, Japan. [Hirooka, Yuuri; Chaverri, Priscila] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. RP Hirooka, Y (reprint author), Univ Maryland, Dept Plant Sci & Landscape Architecture, 2112 Plant Sci Bldg, College Pk, MD 20742 USA. EM Yuuri.Hirooka@ars.usda.gov OI Chaverri, Priscila/0000-0002-8486-6033 FU United States National Science Foundation (NSF) [DEB-0731510] FX This study was supported in part by the United States National Science Foundation (NSF) PEET grant DEB-0731510 Monographic studies in the Nectriaceae, Hypocreales: Nectria, Cosmospora and Neomectria to University of Maryland (PI: P. Chaverri, A.Y. Rossman, G.J. Samuels). NR 32 TC 8 Z9 9 U1 1 U2 3 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2010 VL 102 IS 2 BP 418 EP 429 DI 10.3852/09-137 PG 12 WC Mycology SC Mycology GA 571HW UT WOS:000275743900015 PM 20361508 ER PT J AU Bruns, TD Grubisha, LC Trappe, JM Kerekes, JF Vellinga, EC AF Bruns, Thomas D. Grubisha, Lisa C. Trappe, James M. Kerekes, Jennifer F. Vellinga, Else C. TI Suillus quiescens, a new species commonly found in the spore bank in California and Oregon SO MYCOLOGIA LA English DT Article DE bioassay; suilloid-specific primers; Suillus brevipes; S. occidentalis; S. volcanalis ID ECTOMYCORRHIZAL FUNGI; RHIZOPOGON; SEQUENCES; SEEDLINGS; IDENTIFICATION; FORESTS; HOSTS AB Suillus quienscens sp. nov. is common under Pinus muricata on Santa Cruz and Santa Rosa Islands in the northern Channel Islands of California, and we subsequently found it fruiting at Point Reyes National Seashore on the central coast of California. Sequence from the internal transcribed spacer region show that it is distinct from all 44 species of Suillus tested, and features of its morphology separate it from all other unsequenced species. Suillus quiescens has a broader distribution than coastal California because it also was encountered as ectomycorrhizae on roots of pine seedlings from the eastern Sierra Nevada, coastal Oregon and the southern Cascade mountains. The reason it had not been identified from the se areas might be due to its resemblance to S. brevipes at maturity or it might be a rare fruiter that persists in the spot-e bank. C1 [Bruns, Thomas D.; Kerekes, Jennifer F.; Vellinga, Else C.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Grubisha, Lisa C.] Univ Arizona, USDA ARS, Dept Plant Sci, Tucson, AZ 85721 USA. [Trappe, James M.] Oregon State Univ, Dept Forest Sci, Corvallis, OR 97331 USA. RP Bruns, TD (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM pogon@berkeley.edu FU NSF [DEB-0618293] FX Financial support was supplied in part by NSF DEB-0618293 to TDB and ECV. We dedicate this work to Harry Thiers, whose command of the western North American Suillus species remains unsurpassed. NR 23 TC 8 Z9 8 U1 0 U2 11 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2010 VL 102 IS 2 BP 438 EP 446 DI 10.3852/09-149 PG 9 WC Mycology SC Mycology GA 571HW UT WOS:000275743900017 PM 20361510 ER PT J AU Desjardin, DE Perry, BA Lodge, DJ Stevani, CV Nagasawa, E AF Desjardin, Dennis E. Perry, Brian A. Lodge, D. Jean Stevani, Cassius V. Nagasawa, Eiji TI Luminescent Mycena: new and noteworthy species SO MYCOLOGIA LA English DT Article DE Agaricales; bioluminescence; Mycenaceae; mycenoid fungi; taxonomy ID AGARICALES AB Seven species or Mycena are reported as luminescent, representing specimens collected in Belize, Brazil, Dominican Republic, Jamaica, japan (Bonin Islands), Malaysia (Borneo) and Puerto Rico. Four of them represent new species (Mycena luxaeterna, M. luxarboricola, M. luxperpetua, M. silvaelucens) and three represent new reports of luminescence in previously described species (M. aff. abieticola, M. aspratilis, M. margarita). Mycena subepipterygia is synonymized with M. margarita, and M. chlorinosma is proposed as a possible synonym. Comprehensive descriptions, illustrations, photographs and comparisons with phenetically similar species are provided. A redescription of M. chlorophos, based on analyses of type specimens and recently collected topotypical material, is provided. The addition of these seven new or new, reported luminescent species of Mycena brings the total to 71 known bioluminescent species of fungi. C1 [Desjardin, Dennis E.; Perry, Brian A.] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA. [Lodge, D. Jean] US Forest Serv, USDA, No Res Stn, Luquillo, PR 00773 USA. [Stevani, Cassius V.] Univ Sao Paulo, Inst Quim, BR-05599970 Sao Paulo, Brazil. [Nagasawa, Eiji] Tottori Mycol Inst, Tottori 6891125, Japan. RP Desjardin, DE (reprint author), San Francisco State Univ, Dept Biol, 1600 Holloway Ave, San Francisco, CA 94132 USA. EM ded@sfsu.edu RI Stevani, Cassius/B-9465-2012; Cepema, Inct/I-2429-2013; Desjardin, Dennis/D-4201-2013; Institute of Chemistry - USP, Dept. of Chemistry/B-8988-2012 FU National Science Foundation [DEB-0542445]; Lodge and T. J. Baroni [DEB-9525902, DEB-0103621]; Lodge among others [DEB-0218039]; National Geographic Society [8240-07]; Maya Mountains in Belize; FAPESP [06/53628-3] FX This research was financed in part by National Science Foundation grants to D.E. Desjardin and J.-M. Moncalvo (DEB-0542445). to D.J. Lodge and T. J. Baroni (DEB-9525902 and DEB-0103621). to D.J. Lodge among others (DEB-0218039), and a National Geographic Society grant (No. 8240-07) to T.J. Baroni for collections from the Maya Mountains in Belize, We thank FAPESP (Grant No. 06/53628-3) for providing financial support to C.V. Stevani for fieldworl in Brazil. NR 33 TC 16 Z9 19 U1 3 U2 28 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2010 VL 102 IS 2 BP 459 EP 477 DI 10.3852/09-197 PG 19 WC Mycology SC Mycology GA 571HW UT WOS:000275743900020 PM 20361513 ER PT J AU De Respinis, S Vogel, G Benagli, C Tonolla, M Petrini, O Samuels, GJ AF De Respinis, Sophie Vogel, Guido Benagli, Cinzia Tonolla, Mauro Petrini, Orlando Samuels, Gary J. TI MALDI-TOF MS of Trichoderma: a model system for the identification of microfungi SO MYCOLOGICAL PROGRESS LA English DT Article DE Taxonomy; MALDI-TOFMS; Mass spectrometry; Hypocreales ID TIME-OF-FLIGHT; POLYPEPTIDE ANTIBIOTICS PEPTAIBIOTICS; MASS-SPECTROMETRY; GENUS TRICHODERMA; FUTURE-PROSPECTS; HYPOCREA; LONGIBRACHIATUM; SPORES; DIVERSITY; PHYLOGENY AB This investigation aimed to assess whether MALDI-TOF MS analysis of the proteome could be applied to the study of Trichoderma, a fungal genus selected because it includes many species and is phylogenetically well defined. We also investigated whether MALDI-TOF MS analysis of peptide mass fingerprints would reveal apomorphies that could be useful in diagnosing species in this genus. One hundred and twenty nine morphologically and genetically well-characterized strains of Hypocrea and Trichoderma, belonging to 25 species in 8 phylogenetic clades, were analyzed by MALDI-TOF MS mass spectrometry. The resulting peak lists of individual samples were submitted to single-linkage cluster analysis to produce a taxonomic tree and were compared to ITS and tef1 sequences from GenBank. SuperSpectra (TM) for the 13 most relevant species of Trichoderma were computed. The results confirmed roughly previously defined clades and sections. With the exceptions of T. saturnisporum (Longibrachiatum Clade) and T. harzianum ( Harzianum Clade), strains of individual species clustered very closely. T. polysporum clustered distantly from all other groups. The MALDI-TOF MS analysis accurately reflected the phylogenetic classification reported in recent publications, and, in most cases, strains identified by DNA sequence analysis clustered together by MALDI-TOF MS. The resolution of MALDI-TOF MS, as performed here, was roughly equivalent to ITS rDNA. The MALDI-TOF MS technique analyzes peptides and represents a rough equivalent to sequencing, making this method a useful adjunct for determination of species limits. It also allows simple, reliable, and quick species identification, thus representing a valid alternative to gene sequencing for species diagnosis of Trichoderma and other fungal taxa. C1 [De Respinis, Sophie; Benagli, Cinzia; Tonolla, Mauro; Petrini, Orlando] Cantonal Inst Microbiol, CH-6500 Bellinzona, Switzerland. [Vogel, Guido] Mabritec AG, CH-4002 Basel, Switzerland. [Tonolla, Mauro] Univ Geneva, Dept Plant Biol, Microbial Ecol Lab, Microbiol Unit, CH-1211 Geneva, Switzerland. [Samuels, Gary J.] USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP De Respinis, S (reprint author), Cantonal Inst Microbiol, Via Mirasole 22A, CH-6500 Bellinzona, Switzerland. EM sophie.derespinis@ti.ch NR 67 TC 33 Z9 34 U1 1 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-416X J9 MYCOL PROG JI Mycol. Prog. PD MAR PY 2010 VL 9 IS 1 BP 79 EP 100 DI 10.1007/s11557-009-0621-5 PG 22 WC Mycology SC Mycology GA 575PC UT WOS:000276078400008 ER PT J AU Jaramillo, J Chapman, EG Vega, FE Harwood, JD AF Jaramillo, Juliana Chapman, Eric G. Vega, Fernando E. Harwood, James D. TI Molecular diagnosis of a previously unreported predator-prey association in coffee: Karnyothrips flavipes Jones (Thysanoptera: Phlaeothripidae) predation on the coffee berry borer SO NATURWISSENSCHAFTEN LA English DT Article DE Biological control; Organic coffee; Karnyothrips flavipes; Hypothenemus hampei; Gut-content analysis; Kenya; Predator-prey interactions ID CONSERVATION BIOLOGICAL-CONTROL; HYPOTHENEMUS-HAMPEI COLEOPTERA; GUT-CONTENT ANALYSIS; NATURAL ENEMIES; GENERALIST PREDATORS; APHID PREDATION; TROPHIC LINKS; FOOD-WEBS; BIODIVERSITY; PLANTATIONS AB The coffee berry borer, Hypothenemus hampei, is the most important pest of coffee throughout the world, causing losses estimated at US $500 million/year. The thrips Karnyothrips flavipes was observed for the first time feeding on immature stages of H. hampei in April 2008 from samples collected in the Kisii area of Western Kenya. Since the trophic interactions between H. hampei and K. flavipes are carried out entirely within the coffee berry, and because thrips feed by liquid ingestion, we used molecular gut-content analysis to confirm the potential role of K. flavipes as a predator of H. hampei in an organic coffee production system. Species-specific COI primers designed for H. hampei were shown to have a high degree of specificity for H. hampei DNA and did not produce any PCR product from DNA templates of the other insects associated with the coffee agroecosystems. In total, 3,327 K. flavipes emerged from 17,792 H. hampei-infested berries collected from the field between April and September 2008. Throughout the season, 8.3% of K. flavipes tested positive for H. hampei DNA, although at times this figure approached 50%. Prey availability was significantly correlated with prey consumption, thus indicating the potential impact on H. hampei populations. C1 [Jaramillo, Juliana] ICIPE, Nairobi, Kenya. [Jaramillo, Juliana] Leibniz Univ Hannover, Inst Plant Dis & Plant Protect, D-30419 Hannover, Germany. [Chapman, Eric G.; Harwood, James D.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Vega, Fernando E.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. RP Jaramillo, J (reprint author), ICIPE, POB 30772-00100, Nairobi, Kenya. EM jjaramillo@icipe.org OI Vega, Fernando E./0000-0001-8103-5640 FU German Research Foundation-Deutsche Forschungsgemeinschaft (DFG); University of Kentucky [KY008043] FX We are very grateful to Mr. Charles Kamonjo (icipe) for technical assistance, Adenirin Chabi- Olaye (icipe) for statistical advice, Christian Borgemeister for helpful comments to an earlier version of the manuscript, and Steve Nakahara (USDA, ARS) for identifying the thrips. We thank Abel O. Mainya from Kisii Coffee Demonstration (Kenya) for kindly providing the coffee berries and for his help during the sampling period, Prof. Linda Field (Head of Insect Molecular Biology Group Rothamsted Research) and Prof. John A. Pickett (Head Department of Biological Chemistry, Rothamsted Research) and three anonymous reviewers who provided valuable comments on an earlier draft of the manuscript. This research was funded by the German Research Foundation-Deutsche Forschungsgemeinschaft (DFG). JDH and ECG are supported by the University of Kentucky Agricultural Experiment Station State Project KY008043. This is publication number 09-08-052 of the University of Kentucky Agricultural Experiment Station. NR 69 TC 16 Z9 20 U1 0 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0028-1042 EI 1432-1904 J9 NATURWISSENSCHAFTEN JI Naturwissenschaften PD MAR PY 2010 VL 97 IS 3 BP 291 EP 298 DI 10.1007/s00114-009-0641-7 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 553YT UT WOS:000274403400006 PM 20094879 ER PT J AU Andrews, DM Barton, CD Czapka, SJ Kolka, RK Sweeney, BW AF Andrews, Danielle M. Barton, Christopher D. Czapka, Stephen J. Kolka, Randall K. Sweeney, Bernard W. TI Influence of tree shelters on seedling success in an afforested riparian zone SO NEW FORESTS LA English DT Article DE Riparian restoration; Tree shelters; Seedling growth; Debris accumulation ID FOREST RESTORATION; PERSPECTIVE; GROWTH; HERBIVORY; STREAM; SITE AB The restoration of a natural riparian ecosystem is a key component to improving water quality and restoring stream health in a disturbed watershed. The rate and degree of riparian restoration, and hence stream restoration, depends in part upon afforestation practices. Successful afforestation is determined largely by the rates of survivorship and growth of the tree species planted or recruited in a riparian zone. This study was part of a project involving the restoration of a channelized section of Wilson Creek located in the Bernheim Arboretum and Research Forest, Nelson County, Kentucky. Riparian restoration activities focused on reestablishing a native riparian corridor using American sycamore (Platanus occidentalis), green ash (Fraxinus pennsylvanica var. subintegerrima), and pin oak (Quercus palustris). This study evaluated techniques for improving the growth and survivorship of planted seedlings along Wilson Creek. Specifically, two tree shelter types (Tubex(A (R)) vs. continental mesh), with or without herbicide treatments, were compared. Additionally, the influence of these techniques on debris retention within the riparian zone was also examined. Results showed that use of tree shelters significantly increased the growth of seedlings (but not survivorship), provided physical protection especially during heavy flooding events, and accelerated woody debris retention. Both Tubex(A (R)) and continental mesh tree shelters were effective in enhancing seedling growth, with Tubex(A (R)) shelters yielding significantly better growth when combined with herbicide to control competing vegetation. Thus, the most cost effective choice may depend on the environmental setting and ability to combine the shelters with other growth enhancing treatments. C1 [Andrews, Danielle M.; Barton, Christopher D.] Univ Kentucky, Dept Forestry, Lexington, KY 40546 USA. [Czapka, Stephen J.] Ecol & Environm Inc, Virginia Beach, VA USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. [Sweeney, Bernard W.] Stroud Water Res Ctr, Avondale, PA USA. RP Barton, CD (reprint author), Univ Kentucky, Dept Forestry, 203 Thomas Poe Cooper Bldg, Lexington, KY 40546 USA. EM barton@uky.edu NR 28 TC 5 Z9 6 U1 4 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 J9 NEW FOREST JI New For. PD MAR PY 2010 VL 39 IS 2 BP 157 EP 167 DI 10.1007/s11056-009-9161-8 PG 11 WC Forestry SC Forestry GA 555WZ UT WOS:000274548700003 ER PT J AU Motsinger, JR Kabrick, JM Dey, DC Henderson, DE Zenner, EK AF Motsinger, Jonathan R. Kabrick, John M. Dey, Daniel C. Henderson, Dawn E. Zenner, Eric K. TI Effect of midstory and understory removal on the establishment and development of natural and artificial pin oak advance reproduction in bottomland forests SO NEW FORESTS LA English DT Article DE Midstory removal; Photosynthetically-active radiation; Natural regeneration; Artificial regeneration; Underplanting ID PAGODA RAF. SEEDLINGS; EASTERN UNITED-STATES; NORTHERN RED OAK; SOUTHERN ILLINOIS; QUERCUS-RUBRA; PARTIAL OVERSTORY; WOODY VEGETATION; GROWTH; STANDS; LIGHT AB Regenerating oaks (Quercus L.) on mesic and hydric sites has remained a problem largely because of inadequate density and poor distribution of large oak advance reproduction prior to harvesting. We examined the effect of midstory and understory removal on the establishment and 3-year development of natural and artificial sources of pin oak (Q. palustris Muenchh.) advance reproduction in bottomland forests in southeastern Missouri, USA. Midstory and understory removals increased the photosynthetically-active radiation (PAR) reaching the seedling layer from about 3 to 15%. This increased light did not increase the density of natural pin oak advance reproduction compared to control, but it increased the survival and nominally increased the growth of the natural pin oak advance reproduction. Where the midstory and understory had been removed, underplanted RPM(A (R)) container stock and bareroot pin oak stock maintained high survival, but of the two only the RPM(A (R)) stock maintained positive height and diameter growth while bareroot stock suffered some growth reductions. Pin oaks originating from the direct seeding of stratified acorns sown in the spring had low germination and survival, but the survivors had growth rates similar to those of natural seedlings in thinned stands. Applying triclopyr to competitors in the ground flora layer only nominally increased PAR but reduced the percent survival and marginally increased the growth of natural and artificial pin oak. We conclude that artificial reproduction may be used to further increase the probability of achieving adequate numbers of the desired species in the future. Bareroot seedlings may not perform as well as RPM(A (R)) seedlings and natural seedlings already present. However, bareroot and RPM(A (R)) seedlings remained significantly larger than the natural seedlings after 3 years. C1 [Kabrick, John M.; Dey, Daniel C.] US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. [Motsinger, Jonathan R.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Henderson, Dawn E.] Open Rivers & Wetlands Field Stn, Missouri Dept Conservat, Jackson, MO 63755 USA. [Zenner, Eric K.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. RP Kabrick, JM (reprint author), US Forest Serv, USDA, No Res Stn, 202 Nat Resources Bldg, Columbia, MO 65211 USA. EM jkabrick@fs.fed.us FU Missouri Department; USDA Forest Service, Northern Research Station FX We thank Nicholas Krekeler (St. Charles County Parks), Michael Anderson and David Wissehr (Missouri Department of Conservation) for assistance establishing this study and Dr. Michael Wallendorf (Missouri Department of Conservation) and Dr. Mark Ellersieck (University of Missouri) for assistance with statistical analyses. We also thank the two anonymous reviewers for their helpful comments. Funding for this project was provided by the Missouri Department and the USDA Forest Service, Northern Research Station. NR 62 TC 14 Z9 14 U1 2 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 J9 NEW FOREST JI New For. PD MAR PY 2010 VL 39 IS 2 BP 195 EP 213 DI 10.1007/s11056-009-9164-5 PG 19 WC Forestry SC Forestry GA 555WZ UT WOS:000274548700006 ER PT J AU Larouche, C Kenefic, LS Ruel, JC AF Larouche, Catherine Kenefic, Laura S. Ruel, Jean-Claude TI Northern White-Cedar Regeneration Dynamics on the Penobscot Experimental Forest in Maine: 40-Year Results SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE partial cutting; recruitment; browsing; growth; eastern white cedar ID ACADIAN FOREST; SILVICULTURAL SYSTEMS; BOREAL FOREST; FIRE HISTORY; STANDS; QUEBEC; GROWTH; DEER; CONIFERS; MICHIGAN AB The objective of this study was to assess the long-term dynamics of northern white-cedar (Thuja occidentalis L.) seedling and sapling growth and mortality on the Penobscot Experimental Forest in Maine. Data collected between 1965 and 2005 in four twice-replicated partial cutting treatments were analyzed. White-cedar seedlings established in all treatments despite relatively high white tailed-deer (Odocoileus virginianus Zimmerman) population densities. However, although it appears that regeneration cohorts of associated softwoods increased in size over time, the white-cedar cohort did not. Ingrowth of white-cedar from the seedling to sapling stage was lower than the combined rates of sapling mortality and recruitment to the pole stage; sapling density of this species in 2005 was >80% less than it was at the start of the measurement period. Sapling mortality was high, and recruitment to larger size classes was low, although mortality decreased and recruitment increased as sapling size increased. Browsing was prolific; 90% of white-cedar seedlings and small saplings showed signs of browse in 2005. Overall, white-cedar sapling growth was slow, with an estimated 100 years needed to grow from small sapling to merchantable size in the study stands. Efforts to release white-cedar saplings through precommercial treatment and control of browsing pressure are recommended. C1 [Larouche, Catherine] Minist Nat Resources, Direct Rech Forestiere, Quebec City, PQ G1P 3W8, Canada. [Kenefic, Laura S.] US Forest Serv, No Stn, Bradley, ME 04411 USA. [Ruel, Jean-Claude] Univ Laval, Dept Sci Bois & Foret, Quebec City, PQ G1K 7P4, Canada. RP Larouche, C (reprint author), Minist Nat Resources, Direct Rech Forestiere, 2700 Rue Einstein,Bur B1-150, Quebec City, PQ G1P 3W8, Canada. EM catherine.larouche@mrnf.gouv.qc.ca RI Kenefic, Laura/H-3177-2014 FU Fonds quebecois de la recherche slur la nature et les technologies (FQRNT); US. Forest Service; Northern Research Station; Natural Sciences and Engineering Research Council of Canada (NSERC); CFS; CEF; Fonds F.K Morrow; MRNF (DRF) FX Catherine Laroztche (catherine.larouche@mrnf.gouv.qc.ca), Ministry of Natural Resources Quebec, Direction de la recherche forestiere, 2700 rue Einstein, bur. B.I.150, Quebec, QC GIP 3W8, Canada. Laura S. Kenefic, US Forest Service, Northern Station, 686 Government Rd., Bradley, ME 04411. Jean-Claude Ruel, Department des sciences du bois et de foret, Universite Laval Quebec, QC G1K7P4, Canada. This study was funded by the Fonds quebecois de la recherche slur la nature et les technologies (FQRNT), and was made possible through support from the US. Forest Service, Northern Research Station. The authors thank John C. Brissette from the US. Forest Service for his support. They also thank Mathieu Fortin and Isabelle Auger from the Ministere des Ressotores naturelle et de la Faune du Quebec (MRNF)-Direction de la recherche forestiere (DRF) and Aaron Weiskittel from the University of Maine for their help with statistical analyses. Catherine Larouche was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC), the CFS, the CEF, the Fonds F.K Morrow, and the MRNF (DRF). We are grateful to all those persons who contributed to the project. NR 51 TC 8 Z9 8 U1 1 U2 8 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 J9 NORTH J APPL FOR JI North. J. Appl. For. PD MAR PY 2010 VL 27 IS 1 BP 5 EP 12 PG 8 WC Forestry SC Forestry GA 578NJ UT WOS:000276300200002 ER PT J AU Westfall, JA AF Westfall, James A. TI New Models for Predicting Diameter at Breast Height from Stump Dimensions SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE mixed-effects model; stand reconstruction; heterogeneous variance; prediction interval ID VARIANCE; VOLUME AB Models to predict dbh from stump dimensions are presented for 18 species groups. Data used to fit the models were collected across thirteen states in the northeastern United States. Primarily because of the presence of multiple measurements from each tree, a mixed-effects modeling approach was used to account for the lack of independence among observations. The heterogeneous error variance was described as a function of stump diameter, which allowed for more accurate representation of prediction intervals. Application of the mean response model (fixed-effects parameters only) to independent data indicated an average absolute error between 0.2 and 0.7 in. for most groups. An additional advantage is that random-effect parameters allow the model to be calibrated to local conditions if some additional data are available. An example is provided that indicates the local calibration results in a mean residual value that is closer to zero compared with the mean response model. Efforts in other locales to use stump information to inform dbh predictions can obtain the same advancements by adopting a similar modeling methodology. C1 US Forest Serv Forest Inventory & Anal, No Res Stn, Newtown Sq, PA 19073 USA. RP Westfall, JA (reprint author), US Forest Serv Forest Inventory & Anal, No Res Stn, 11 Campus Blvd,Ste 200,Newtown Sq, Newtown Sq, PA 19073 USA. EM jameswestfall@fs.fed.us NR 21 TC 8 Z9 8 U1 1 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 J9 NORTH J APPL FOR JI North. J. Appl. For. PD MAR PY 2010 VL 27 IS 1 BP 21 EP 27 PG 7 WC Forestry SC Forestry GA 578NJ UT WOS:000276300200004 ER PT J AU Zielinski, WJ Hunter, JE Hamlin, R Slauson, KM Mazurek, MJ AF Zielinski, William J. Hunter, John E. Hamlin, Robin Slauson, Keith M. Mazurek, M. J. TI Habitat Characteristics at Den Sites of the Point Arena Mountain Beaver (Aplodontia rufa nigra) SO NORTHWEST SCIENCE LA English DT Article ID PRIMITIVE RODENT; SELECTION; EFFICACY AB The Point Arena mountain beaver (Aplodontia rufa nigra) is a federally listed endangered species, but has been the subject of few studies. Mountain beavers use burrows that include a single subterranean den. Foremost among the information needs for this subspecies is a description of the above-ground habitat features associated with dens. Using telemetry we located dens of 23 individuals in Manchester State Park, Mendocino County, California. We measured vegetation and topographic variables directly above the dens and at two available sites within the same burrow system. Alternative resource selection functions, based on multivariate expressions of important ecological characteristics, were developed to model features associated with dens. The best model contained three variables: MEANDENS (mean vegetation density), PAMBTOP4 (cover of the four plant species most frequently used), and COSASPECT (cosine aspect). Interestingly, PAMBTOP4 was negatively associated with dens, indicating that dens were not chosen for their proximity to important plant species. Topography plays an important role in that western and northern aspects were favored and SLOPE was included in the second-highest ranked model. Cross validation indicated moderate stability for the top model suggesting that potentially important predictors that were excluded from the analysis (e.g., soil characteristics, social context) may be influential. Nonetheless, we demonstrated that dense vegetation and aspect/slope considerations are more important predictors of Point Arena mountain beaver den selection than proximity to cover of important plant species. Our results apply to Point Arena mountain beaver populations in coastal shrub communities; den selection may be different farther inland, in forests. C1 [Zielinski, William J.; Slauson, Keith M.; Mazurek, M. J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. [Hunter, John E.; Hamlin, Robin] US Fish & Wildlife Serv, USDI, Arcata Fish & Wildlife Off, Arcata, CA 95521 USA. RP Zielinski, WJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Redwood Sci Lab, 1700 Bayview Dr, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU USDI Fish and Wildlife Service; Arcata Fish and Wildlife Office; USDA Forest Service, Pacific Southwest Research Station FX This project was funded by the USDI Fish and Wildlife Service, Arcata Fish and Wildlife Office and the USDA Forest Service, Pacific Southwest Research Station. We thank Kim Fitts of BioConsultant LLC for sharing her knowledge and insight about Point Arena mountain beaver ecology. Renee Pasquinelli of the California Department of Parks and Recreation facilitated access to Manchester State Park and provided other logistical support. Peter Warner of the California Department of Parks and Recreation assisted with plant identification. Amedee Brickey of the USDI Fish and Wildlife Service also provided support. Dale Nolte and Wendy Arjo of the USDA National Wildlife Research Center generously shared their time and expertise in training us to capture and handle mountain beavers. Lowell Diller and Keith Hamm of Green Diamond Resource Company facilitated pilot studies on the northern California subspecies of mountain beaver. We thank Janet Werren and Kaytee Redfern for help with tables and the figure. NR 52 TC 3 Z9 3 U1 2 U2 24 PU WASHINGTON STATE UNIV PI PULLMAN PA PO BOX 645020, PULLMAN, WA 99164-5910 USA SN 0029-344X J9 NORTHWEST SCI JI Northwest Sci. PD SPR PY 2010 VL 84 IS 2 BP 119 EP 130 PG 12 WC Ecology SC Environmental Sciences & Ecology GA 604FD UT WOS:000278261800001 ER PT J AU Gervais, JA Griffith, SM Davis, JH Cassidy, JR Dragila, MI AF Gervais, Jennifer A. Griffith, Stephen M. Davis, Jennifer H. Cassidy, James R. Dragila, Maria I. TI Effects of Gray-tailed Vole Activity on Soil Properties SO NORTHWEST SCIENCE LA English DT Article ID NITROGEN DYNAMICS; SMALL MAMMALS; GRASSLAND; POPULATIONS; HERBIVORES; VEGETATION; DEMOGRAPHY; RESPONSES; BURROWS; CYCLES AB Voles are well-known crop pests, especially when peak populations are present, but their role in soil fertility and impacts on agricultural sustainability are not well understood. Five months after the abrupt disappearance of a peak in a gray-tailed vole (Microtus canicaudus) population, we examined burrow structure, determined concentrations of trace elements, carbon and nitrogen in the soil immediately surrounding vole burrows, and compared soil chemical properties to a depth of 90 cm between areas with prior vole activity and areas of no activity. Vole tunneling activity was confined to the top 10 cm of the soil profile and was coincident with the majority of root biomass. Soil NH(4)(+), NO(3)(-), extractable organic carbon, and soil organic matter were greater below vole tunnels than above; however, due to small sample sizes, differences were not significant There were no differences in trace elements with respect to position around vole tunnels. Vole activity was associated with increased soil nitrate concentrations and decreased soil pH to a depth of 90 cm, indicating that nitrification might be enhanced by vole activity, and that this effect continues after vole populations crash. Greater inorganic nitrogen could have long-term effects on ecosystem productivity. The effects voles have on soil processes that influence carbon and nutrient cycle requires further investigation. C1 [Gervais, Jennifer A.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97330 USA. [Griffith, Stephen M.; Davis, Jennifer H.] ARS, USDA, Corvallis, OR 97330 USA. [Cassidy, James R.; Dragila, Maria I.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97330 USA. RP Gervais, JA (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97330 USA. EM Jennifer.Gervais@oregonstate.edu NR 42 TC 5 Z9 5 U1 3 U2 16 PU WASHINGTON STATE UNIV PI PULLMAN PA PO BOX 645020, PULLMAN, WA 99164-5910 USA SN 0029-344X J9 NORTHWEST SCI JI Northwest Sci. PD SPR PY 2010 VL 84 IS 2 BP 159 EP 169 PG 11 WC Ecology SC Environmental Sciences & Ecology GA 604FD UT WOS:000278261800005 ER PT J AU Hard, JS Zogas, KP AF Hard, John S. Zogas, Ken P. TI Old Lower Stem Bark Lesions Apparently Caused by Unsuccessful Spruce Beetle Attacks Still Evident on Live Spruce Trees Years Later SO NORTHWEST SCIENCE LA English DT Article ID SOUTH-CENTRAL ALASKA; WHITE SPRUCE AB We examined old bark lesions on Lutz spruce in young stands on the Kenai Peninsula, Alaska, to determine their cause. Distribution of these lesions along lower stems was similar to the distribution of spruce beetle attacks during epidemics. These lesions apparently resulted from unsuccessful attacks by spruce beetles during the late 1980s and early 1990s and appear to be symptomatic of conifer tree resistance reactions to cambial zone injury by Dendroctonus beetles. C1 [Hard, John S.; Zogas, Ken P.] US Forest Serv, USDA, Forest Hlth Protect & Pacific NW Res Stn, Anchorage, AK 99503 USA. RP Hard, JS (reprint author), US Forest Serv, USDA, Forest Hlth Protect & Pacific NW Res Stn, Anchorage, AK 99503 USA. EM jkhard@gci.net NR 7 TC 0 Z9 0 U1 0 U2 0 PU WASHINGTON STATE UNIV PI PULLMAN PA PO BOX 645020, PULLMAN, WA 99164-5910 USA SN 0029-344X J9 NORTHWEST SCI JI Northwest Sci. PD SPR PY 2010 VL 84 IS 2 BP 192 EP 195 PG 4 WC Ecology SC Environmental Sciences & Ecology GA 604FD UT WOS:000278261800008 ER PT J AU Flagg, RO Smith, GL Meerow, AW AF Flagg, Raymond O. Smith, Gerald L. Meerow, Alan W. TI New Combinations in Habranthus (Amaryllidaceac) in Mexico and Southwestern USA SO NOVON LA English DT Article DE Amaryllidaceae; Habranthus; Mexico; southwestern United States; Zephyranthes AB . New comhinations in Habranthus Herb. (Amaryllidaceae) are made for four species that were previously treated as Zephyranthes Herb. (Z. arenicola Brandegee, Z. chichimeca T. M. Howard & S. Ogden, Z. conzottii Greenm., and Z. longifolia Hemsl.) and that are endemic to Mexico and the southwestern United States: H. arenicola (Brandegee) Flagg, G. Lom. Sm. & Meerow, H. chichimeca (T. M. Howard & S. Ogden) Flagg, C. Lom. Sm. & Meerow, H. conzattii (Greemn.) Flagg, G. Lom. Sm. & Meerow, and H. longifolius (Hemsl.) Flagg, G. Lom. Sm. & Meerow. The mature anthers, which are more or less horizontal and usually Innate or U-shaped, readily distinguish Habranthus from Zephyranthes, in which ale mature anthers are vertical and relatively linear. C1 [Flagg, Raymond O.] Carolina Biol Supply Co, Burlington, NC 27215 USA. [Smith, Gerald L.] High Point Univ, Dept Biol, High Point, NC 27262 USA. [Meerow, Alan W.] USDA ARS, SHRS, Miami, FL 33158 USA. [Meerow, Alan W.] Fairchild Trop Garden, Miami, FL 33158 USA. RP Flagg, RO (reprint author), Carolina Biol Supply Co, 2700 York Rd, Burlington, NC 27215 USA. EM rflagg@triad.rr.com NR 3 TC 3 Z9 4 U1 0 U2 1 PU MISSOURI BOTANICAL GARDEN PI ST LOUIS PA 2345 TOWER GROVE AVENUE, ST LOUIS, MO 63110 USA SN 1055-3177 J9 NOVON JI Novon PD MAR PY 2010 VL 20 IS 1 BP 33 EP 34 DI 10.3417/2008049 PG 2 WC Plant Sciences SC Plant Sciences GA 575JN UT WOS:000276063400007 ER PT J AU Sigua, GC Hubbard, RK Coleman, SW Williams, M AF Sigua, Gilbert C. Hubbard, Robert K. Coleman, Samuel W. Williams, Mimi TI Nitrogen in soils, plants, surface water and shallow groundwater in a bahiagrass pasture of Southern Florida, USA SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Bahiagrass; cow-calf; Groundwater; Inorganic nitrogen; Nutrient cycling; Plant uptake; Water quality ID PERENNIAL GRASSES; CATTLE EXCRETA; NORTHEAST USA; COSTA-RICA; PHOSPHORUS; QUALITY; RUNOFF; MANAGEMENT; URINE; MOVEMENT AB Despite substantial measurements using both laboratory and field techniques, little is known about the spatial and temporal variability of nitrogen (N) dynamics across the landscapes, especially in agricultural landscapes with cow-calf operations. This study was conducted to assess the comparative levels of total inorganic nitrogen, TIN (NO(3)-N + NH(4)-N) among soils, forage, surface water and shallow groundwater (SGW) in bahiagrass (Paspalum notatum, Fluegg,) pastures. Soil samples were collected at 0-20, 20-40, 40-60, and 60-100 cm across the pasture's landscape (top slope, TS; middle slope, MS; and bottom slope, BS) in the spring and fall of 2004, 2005 and 2006, respectively. Bi-weekly (2004-2006) groundwater and surface water samples were taken from wells located at TS, MS, and BS and from the run-off/seepage area (SA). Concentrations of NH(4)-N, NO(3)-N, and TIN in SGW did not vary with landscape position (LP). However, concentrations of NH(4)-N, NO(3)-N, and TIN in water samples collected from the seep area were significantly (P a parts per thousand currency sign 0.05) higher when compared to their average concentrations in water samples collected from the different LP. Average concentrations of NO(3)-N (0.4-0.9 mg l(-1)) among the different LP were well below the maximum, of 10 mg l(-1), set for drinking water. The maximum NO(3)-N concentrations (averaged across LP) in SGW for 2004, 2005 and 2006 were also below the drinking water standards for NO(3)-N. Concentration of TIN in soils varied significantly (P a parts per thousand currency sign 0.05) with LP and soil depth. Top slope and surface soil (0-20 cm) had the greatest concentrations of TIN. The greatest forage availability of 2,963 +/- A 798 kg ha(-1) and the highest N uptake of 56 +/- A 12 kg N ha(-1) were observed from the TS in 2005. Both forage availability and N uptake of bahiagrass at the BS were consistently the lowest when averaged across LP and years. These results can be attributed to the grazing activities as animals tend to graze more at the BS. The average low soil test value of TN (across LP and soil depth) in our soils of 10.9 mg kg(-1) (5.5 kg N ha(-1)) would indicate that current pasture management including cattle rotation in terms of grazing days and current fertilizer application (inorganic + feces + urine) for bahiagrass pastures may not have negative impact on the environment. C1 [Sigua, Gilbert C.; Coleman, Samuel W.] ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. [Hubbard, Robert K.] ARS, USDA, SE Watershed Res Lab, Tifton, GA 31793 USA. [Williams, Mimi] Nat Resources & Conservat Serv, USDA, Gainesville, FL 32606 USA. RP Sigua, GC (reprint author), ARS, USDA, Subtrop Agr Res Stn, Brooksville, FL 34601 USA. EM gilbert.sigua@ars.usda.gov NR 56 TC 8 Z9 9 U1 1 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD MAR PY 2010 VL 86 IS 2 BP 175 EP 187 DI 10.1007/s10705-009-9281-8 PG 13 WC Soil Science SC Agriculture GA 555XE UT WOS:000274549200001 ER PT J AU Rogers, SP Hicks, PD Hamzo, M Veit, LE Abrams, SA AF Rogers, Stefanie P. Hicks, Penni D. Hamzo, Maria Veit, Lauren E. Abrams, Steven A. TI Continuous Feedings of Fortified Human Milk Lead to Nutrient Losses of Fat, Calcium and Phosphorous SO NUTRIENTS LA English DT Article DE calcium; phosphorous; neonates; enteral nutrition; lipids; protein ID BIRTH-WEIGHT INFANTS; BREAST-MILK; PRETERM INFANTS; GROWTH; RATES AB Substantial losses of nutrients may occur during tube (gavage) feeding of fortified human milk. Our objective was to compare the losses of key macronutrients and minerals based on method of fortification and gavage feeding method. We used clinically available gavage feeding systems and measured pre-and post-feeding (end-point) nutrient content of calcium (Ca), phosphorus (Phos), protein, and fat. Comparisons were made between continuous, gravity bolus, and 30-minute infusion pump feeding systems, as well as human milk fortified with donor human milk-based and bovine milk-based human milk fortifier using an in vitro model. Feeding method was significantly associated with fat and Ca losses, with increased losses in continuous feeds. Fat losses in continuous feeds were substantial, with 40 +/- 3 % of initial fat lost during the feeding process. After correction for feeding method, human milk fortified with donor milk-based fortifier was associated with significantly less loss of Ca (8 +/- 4% vs. 28 +/- 4%, p< 0.001), Phos (3 +/- 4% vs. 24 +/- 4%, p < 0.001), and fat (17 +/- 2% vs. 25 +/- 2%, p = 0.001) than human milk fortified with a bovine milk-based fortifier (Mean +/- SEM). C1 [Hicks, Penni D.; Hamzo, Maria; Abrams, Steven A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Rogers, Stefanie P.] NW Newborn Specialists 501 N, Portland, OR 97227 USA. [Veit, Lauren E.] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA. [Abrams, Steven A.] Baylor Coll Med, Dept Pediat, Sect Neonatol, Houston, TX 77030 USA. [Abrams, Steven A.] Texas Childrens Hosp, Houston, TX 77030 USA. RP Abrams, SA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St Houston, Houston, TX 77030 USA. EM stefanie@nwnewborn.com; pennih@bcm.edu; hamzom@stthom.edu; Veitl@rpi.edu; sabrams@bcm.edu OI Abrams, Steven/0000-0003-4972-9233 FU USDA/ARS [58-6250-6-001] FX This work is a publication of the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Texas Children's Hospital, Houston, Texas. This project has been funded in part with federal funds from the USDA/ARS under Cooperative Agreement number 58-6250-6-001. Contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 21 TC 16 Z9 17 U1 1 U2 3 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD MAR PY 2010 VL 2 IS 3 BP 230 EP 240 DI 10.3390/nu2030240 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 864JC UT WOS:000298235100001 PM 22254018 ER PT J AU Junyent, M Tucker, KL Shen, J Lee, YC Smith, CE Mattei, J Lai, CQ Parnell, LD Ordovas, JM AF Junyent, M. Tucker, K. L. Shen, J. Lee, Y. -C. Smith, C. E. Mattei, J. Lai, C. -Q. Parnell, L. D. Ordovas, J. M. TI A composite scoring of genotypes discriminates coronary heart disease risk beyond conventional risk factors in the Boston Puerto Rican Health Study SO NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES LA English DT Article DE Genes; Coronary heart disease; Conventional risk factors; Genetic predisposition score; Single nucleotide polymorphism ID MYOCARDIAL-INFARCTION; CANDIDATE GENES; ARTERY-DISEASE; POLYMORPHISMS; PREDICTION; ATHEROSCLEROSIS; ASSOCIATION; CHOLESTEROL; METABOLISM; MORTALITY AB Background and aims: Using a genetic predisposition score (GPS), integrating the additive associations of a set of single nucleotide polymorphisms (SNPs) with CHD, we examined the consequences of the joint presence of a high GPS and conventional risk factors (CRFs). Methods and results. We studied 11 SNPs at eight loci in 197 participants with prior CHD and 524 CHD-free subjects from the Boston Puerto Rican Health Study. Each polymorphism contributed 1 unit (high-risk allele homozygous), 0.5 units (heterozygous) and 0 units (low-risk allele homozygous) to the GPS. Odds ratio (OR) of CHD for those at high risk because of GPS (>5) and simultaneous presence of CRFs were estimated, compared with subjects at low risk, for both measurements. The mean score was higher in participants with prior CHD than those CHD-free (P = 0.015), and the OR for CHD with a GPS > 5 was 2.90 (P < 0.001). The joint presence of a high GPS and each CRF was associated with higher risk of CHD. Compared to participants with high GPS, those with low GPS (<5) were protected against CHD even if they were smokers (OR = 0.44), heavy drinkers (OR = 0.43), displayed low physical activity (OR = 0.35), had hypertension (OR = 0.52) or hyperlipidemia (OR = 0.34) (P values ranging from 0.004 to 0.023). Conclusion. A simple genetic score of 11 polymorphisms may identify those subjects at increased risk of CHD beyond conventional risk factors. (C) 2009 Elsevier B.V. All rights reserved. C1 [Junyent, M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Sch Med, Boston, MA 02111 USA. RP Junyent, M (reprint author), Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Sch Med, 711 Washington St, Boston, MA 02111 USA. EM mireia.junyent@tufts.edu RI Tucker, Katherine/A-4545-2010; Mattei, Josiemer/H-1800-2016; OI Mattei, Josiemer/0000-0001-5424-8245; Tucker, Katherine/0000-0001-7640-662X; Ordovas, Jose/0000-0002-7581-5680 FU National Institute on Aging [5P01AG023394-02]; NIH/NHLBI [HL54776]; NIH/NIDDK [01(075030]; US Department of Agriculture Research Service; CES [T32 DK007651-19] FX This work was supported by National Institutes of Health, National Institute on Aging, grant number 5P01AG023394-02 and NIH/NHLBI grant number HL54776 and NIH/NIDDK 01(075030 and contracts 53-K06-5-10 and 58-1950-0-001 from the US Department of Agriculture Research Service. MJ is supported by a grant from the Fulbright-Spanish Ministry of Education and Science (reference 2007-1086). CES is supported by grant T32 DK007651-19. None of the authors reported any personal or financial conflict of interest. NR 30 TC 6 Z9 6 U1 0 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0939-4753 J9 NUTR METAB CARDIOVAS JI Nutr. Metab. Carbiovasc. Dis. PD MAR PY 2010 VL 20 IS 3 BP 157 EP 164 DI 10.1016/j.numecd.2009.03.016 PG 8 WC Cardiac & Cardiovascular Systems; Endocrinology & Metabolism; Nutrition & Dietetics SC Cardiovascular System & Cardiology; Endocrinology & Metabolism; Nutrition & Dietetics GA 589BW UT WOS:000277120500002 PM 19501493 ER PT J AU Booth, SL AF Booth, Sarah L. TI Dietary vitamin K guidance: an effective strategy for stable control of oral anticoagulation? SO NUTRITION REVIEWS LA English DT Review DE anticoagulants; diet; phylloquinone; vitamin K; warfarin ID WARFARIN; THERAPY; RISK AB Numerous risk factors for instability of oral anticoagulation have been identified, including variability in vitamin K intake. However, few studies have directly tested the feasibility of manipulating dietary vitamin K to achieve stable oral anticoagulation. Recent findings from a randomized clinical trial suggest that dietary vitamin K manipulation is a viable option for managing stability of oral anticoagulant therapy. The approach appears to be effective for those patients who are under-anticoagulated and consume a small number of vitamin K-rich food sources. While an encouraging option for management of warfarin therapy, longer-term studies in different patient populations are required. (C) 2010 International Life Sciences Institute C1 Tufts Univ, USDA, Human Nutr Res Ctr Aging, Vitamin Lab K, Boston, MA 02111 USA. RP Booth, SL (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Vitamin Lab K, 711 Washington St, Boston, MA 02111 USA. EM sarah.booth@tufts.edu FU U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX Funding. Based upon work supported by the U.S. Department of Agriculture, Agricultural Research Service under Cooperative Agreement no. 58-1950-7-707. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors, and do not necessarily reflect the view of the U. S. Department of Agriculture. NR 15 TC 7 Z9 7 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD MAR PY 2010 VL 68 IS 3 BP 178 EP 181 DI 10.1111/j.1753-4887.2010.00274.x PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 560SK UT WOS:000274922600005 PM 20384848 ER PT J AU Johnston, CA Tyler, C McFarlin, BK Poston, WSC Haddock, CK Reeves, RS Foreyt, JP AF Johnston, Craig A. Tyler, Chermaine McFarlin, Brian K. Poston, Walker S. C. Haddock, C. Keith Reeves, Rebecca S. Foreyt, John P. TI Effects of a School-based Weight Maintenance Program for Mexican-American Children: Results at 2 Years SO OBESITY LA English DT Article ID FAMILY-BASED TREATMENT; BODY-MASS INDEX; CHILDHOOD OBESITY; CONTROLLED-TRIAL; FOLLOW-UP; OVERWEIGHT; ADOLESCENTS; QUALITY; ADULTS; LIFE AB The prevalence of childhood overweight has increased significantly, with the highest rates noted among Mexican Americans. Many negative health outcomes are associated with overweight; thus, there is a need for effective weight-loss interventions tailored to this group. This study evaluated 24-month outcomes of a randomized, controlled trial involving an intensive lifestyle-based weight maintenance program targeting overweight Mexican-American children at a charter school in Houston, Texas. A total of 60 children (33 males, 55%) between the ages of 10 and 14 at or > 85th percentile for BMI were recruited. Participants were randomized to an instructor-led intervention (ILI) or a self-help (SH) program, both aimed at modifying eating and physical activity behaviors using behavior modification strategies. Changes in participants' standardized BMI (zBMI) were assessed at baseline, 1, and 2 years. Tricep skinfold, total cholesterol, triglycerides, high-density lipoprotein cholesterol, and calculated low-density lipoprotein were assessed at baseline and 1 year. ILI participants showed significantly greater decreases in zBMI at 1 and 2 years (F = 26.8, P < 0.001, F = 4.1, P < 0.05, respectively) compared to SH controls. ILI participants showed greater improvements in body composition, as measured by tricep skinfold (F = 9.75, P < 0.01). Children in the ILI condition experienced benefits with respect to total cholesterol (F = 7.19, P < 0.05) and triglycerides (F = 4.35, P < 0.05) compared to children in the SH condition. Overall, the school-based intervention resulted in improved weight and clinical outcomes in overweight Mexican-American children, and zBMI was maintained over 2 years. C1 [Johnston, Craig A.; Tyler, Chermaine; Foreyt, John P.] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat Nutr, Houston, TX 77030 USA. [McFarlin, Brian K.] Univ Houston, Dept Hlth & Human Performance, Houston, TX USA. [Poston, Walker S. C.; Haddock, C. Keith] HOPE Hlth Res Inst, Lees Summit, MO USA. [Reeves, Rebecca S.] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. RP Johnston, CA (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat Nutr, Houston, TX 77030 USA. EM caj@bcm.edu FU United States Department of Agriculture [2533759358] FX We thank the members of the Peanut Institute for their support of this study. We are extremely grateful to the staff of YES Preparatory Academy for their dedication to improving the lives of children. This work was supported by a grant from the United States Department of Agriculture (ARS 2533759358). RCT registration #: NCT00454610. NR 29 TC 24 Z9 25 U1 2 U2 9 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1930-7381 J9 OBESITY JI Obesity PD MAR PY 2010 VL 18 IS 3 BP 542 EP 547 DI 10.1038/oby.2009.241 PG 6 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 562BO UT WOS:000275024100017 PM 19661957 ER PT J AU Bachman, S Heisler-White, JL Pendall, E Williams, DG Morgan, JA Newcomb, J AF Bachman, Sarah Heisler-White, Jana L. Pendall, Elise Williams, David G. Morgan, Jack A. Newcomb, Joanne TI Elevated carbon dioxide alters impacts of precipitation pulses on ecosystem photosynthesis and respiration in a semi-arid grassland SO OECOLOGIA LA English DT Article DE Climate change; Prairie; Net ecosystem carbon dioxide exchange; Carbon cycle; Carbon dioxide flux ID GROSS PRIMARY PRODUCTION; NET PRIMARY PRODUCTION; PLANT WATER RELATIONS; SHORTGRASS STEPPE; PRIMARY PRODUCTIVITY; SOIL RESPIRATION; LONG-TERM; SUMMER PRECIPITATION; CONSTANT FRACTION; DESERT ECOSYSTEMS AB Predicting net C balance under future global change scenarios requires a comprehensive understanding of how ecosystem photosynthesis (gross primary production; GPP) and respiration (Re) respond to elevated atmospheric [CO(2)] and altered water availability. We measured net ecosystem exchange of CO(2) (NEE), GPP and Re under ambient and elevated [CO(2)] in a northern mixed-grass prairie (Wyoming, USA) during dry intervals and in response to simulated precipitation pulse events. Elevated [CO(2)] resulted in higher rates of both GPP and Re across the 2006 growing season, and the balance of these two fluxes (NEE) accounted for cumulative growing season C uptake (-14.4 +/- A 8.3 g C m(-2)). Despite lower GPP and Re, experimental plots under ambient [CO(2)] had greater cumulative uptake (-36.2 +/- A 8.2 g C m(-2)) than plots under elevated [CO(2)]. Non-irrigated control plots received 50% of average precipitation during the drought of 2006, and had near-zero NEE (1.9 +/- A 6.4 g C m(-2)) for the growing season. Elevated [CO(2)] extended the magnitude and duration of pulse-related increases in GPP, resulting in a significant [CO(2)] treatment by pulse day interaction, demonstrating the potential for elevated [CO(2)] to increase the capacity of this ecosystem to respond to late-season precipitation. However, stimulation of Re throughout the growing season under elevated [CO(2)] reduced net C uptake compared to plots under ambient [CO(2)]. These results indicate that although elevated [CO(2)] stimulates gross rates of ecosystem C fluxes, it does not necessarily enhance net C uptake, and that C cycle responses in semi-arid grasslands are likely to be more sensitive to changes in precipitation than atmospheric [CO(2)]. C1 [Bachman, Sarah; Pendall, Elise; Williams, David G.; Newcomb, Joanne] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Pendall, Elise; Williams, David G.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Heisler-White, Jana L.; Williams, David G.] Univ Wyoming, Dept Renewable Resources, Laramie, WY 82071 USA. [Morgan, Jack A.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. RP Pendall, E (reprint author), Univ Wyoming, Dept Bot, 3165,1000 E Univ Ave, Laramie, WY 82071 USA. EM pendall@uwyo.edu RI Williams, David/A-6407-2014; OI Williams, David/0000-0003-3627-5260; Pendall, Elise/0000-0002-1651-8969 FU US Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University; US Department of Agriculture's Cooperative State Research, Education and Extension Service [2008-35107-18655]; USDA-Agricultural Research Service FX This research was supported by the US Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University, and the US Department of Agriculture's Cooperative State Research, Education and Extension Service (grant number 2008-35107-18655), with base support from USDA-Agricultural Research Service. We thank Jo Preston, Meghan Taylor, Dan LeCain, Jennifer Schomp, Peter Koenig, and David Smith for field and lab assistance, David Legg for help with statistical analyses, and Jessica Cable and Lachlan Ingram for useful comments. This experiment complied with the current laws of the USA and state of Wyoming. NR 63 TC 11 Z9 14 U1 4 U2 44 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 J9 OECOLOGIA JI Oecologia PD MAR PY 2010 VL 162 IS 3 BP 791 EP 802 DI 10.1007/s00442-009-1511-x PG 12 WC Ecology SC Environmental Sciences & Ecology GA 555NF UT WOS:000274518000025 PM 19943173 ER PT J AU Lyons, JM Lindholm, E Wang, YZ Thomson, JL Anthony, LB Woltering, EA Frey, DJ Joseph, S Ramacharan, T Boudreaux, JP AF Lyons, John M., III Lindholm, Erica Wang, Yi-Zarn Thomson, Jessica L. Anthony, Lowell B. Woltering, Eugene A. Frey, Daniel J. Joseph, Saju Ramacharan, Thiagaran Boudreaux, J. Philip TI Extensive Retroperitoneal Carcinoid Involving the Mesenteric Vasculature Does Not Preclude Effective Cytoreduction SO PANCREAS LA English DT Meeting Abstract C1 [Lyons, John M., III; Lindholm, Erica; Wang, Yi-Zarn; Frey, Daniel J.; Joseph, Saju; Ramacharan, Thiagaran; Boudreaux, J. Philip] Louisiana State Univ, Hlth Sci Ctr, Dept Surg, Sect Surg Endocrinol, Baton Rouge, LA 70803 USA. [Thomson, Jessica L.] ARS, USDA, So Reg Res Ctr, Baton Rouge, LA USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0885-3177 J9 PANCREAS JI Pancreas PD MAR PY 2010 VL 39 IS 2 BP 274 EP 275 DI 10.1097/01.mpa.0000363921.55478.e6 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA 561WE UT WOS:000275010000049 ER PT J AU Nachman, RJ AF Nachman, Ronald J. TI Invertebrate neuropeptides X SO PEPTIDES LA English DT Editorial Material C1 USDA, Areawide Pest Management Res, So Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Nachman, RJ (reprint author), USDA, Areawide Pest Management Res, So Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM nachman@tamu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PD MAR PY 2010 VL 31 IS 3 SI SI BP 371 EP 371 DI 10.1016/j.peptides.2010.01.014 PG 1 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 574MQ UT WOS:000275994100001 PM 20123113 ER PT J AU Coast, GM TeBrugge, VA Nachman, RJ Lopez, J Aldrich, JR Lange, A Orchard, I AF Coast, Geoffrey M. TeBrugge, Victoria A. Nachman, Ronald J. Lopez, Juan Aldrich, Jeffrey R. Lange, Angela Orchard, Ian TI Neurohormones implicated in the control of Malpighian tubule secretion in plant sucking heteropterans: The stink bugs Acrosternum hilare and Nezara viridula SO PEPTIDES LA English DT Article DE Heteroptera; Pentatomidae; Diuretic hormones; Serotonin; CRF-like peptides; CAPA peptides; Antidiuretic ID HAWKMOTH MANDUCA-SEXTA; RHODNIUS-PROLIXUS STAL; BLOOD-FEEDING BUG; INSECT NEUROPEPTIDE; DIURETIC ACTIVITY; FLUID SECRETION; ANTIDIURETIC HORMONES; ONCOPELTUS-FASCIATUS; ACHETA-DOMESTICUS; HOUSE CRICKET AB Plant sucking heteropteran bugs feed regularly on small amounts of K(+)-rich plant material, in contrast to their hematophagous relatives which imbibe large volumes of Na(+)-rich blood. It was anticipated that this would be reflected in the endocrine control of Malpighian tubule (MT) secretion. To explore this, neuroendocrine factors known to influence MT secretion were tested on MT of the pentatomid plant sucking stink bugs, Acrosternum hilare and Nezara viridula, and the results compared with previously published data from Rhodnius prolixus. Serotonin had no effect on N. viridula MT, although it stimulates secretion by R. prolixus MT >1000-fold, and initiates a rapid diuresis to remove excess salt and water from the blood meal. Kinins had no effect on stink bug MT, but secretion was increased by Zoone-DH, a CRF-like peptide, although the response was a modest 2-3-fold acceleration compared with 1000-fold in R. prolixus. Native CAPA peptides, which have diuretic activity in dipteran flies, had antidiuretic activity in MT of the stink bug (Acrhi/Nezvi-CAPA-1 and -2), as previously shown with Rhopr-CAPA-2 in R. prolixus. The antidiuretic activity of Rhopr-CAPA-2 has been linked with terminating the rapid diuresis, but results with stink bugs suggest it is a general feature of heteropteran MT. (C) 2009 Elsevier Inc. All rights reserved. C1 [Coast, Geoffrey M.] Univ London, Sch Biol & Chem Sci, London WC1E 7HX, England. [TeBrugge, Victoria A.; Lange, Angela; Orchard, Ian] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. [Coast, Geoffrey M.; Nachman, Ronald J.; Lopez, Juan] USDA, Areawide Pest Management Res Unit, So Plains Agr Res Ctr, College Stn, TX 77845 USA. [Aldrich, Jeffrey R.] USDA ARS, Beltsville Agr Res Ctr, Beltsville Agr Res Ctr W, Invas Insects Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Coast, GM (reprint author), Univ London, Sch Biol & Chem Sci, Malet St, London WC1E 7HX, England. EM g.coast@bbk.ac.uk FU North Atlantic Treaty Organization (NATO) [LST.CLG.979226, IS-4205-09C]; Natural Sciences and Engineering Research Council of Canada FX We wish to acknowledge the able technical assistance of Allison Strey and Chris Parker, and the financial support of a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (GMC, KIN) a Binational Agricultural Research and Development Grant (BARD # IS-4205-09C) (RJN), and a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (IO). NR 41 TC 14 Z9 14 U1 1 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PD MAR PY 2010 VL 31 IS 3 SI SI BP 468 EP 473 DI 10.1016/j.peptides.2009.09.017 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 574MQ UT WOS:000275994100014 PM 19772880 ER PT J AU Smagghe, G Mandian, K Zubrzak, P Nachman, RJ AF Smagghe, Guy Mandian, Kamran Zubrzak, Pawel Nachman, Ronald J. TI Antifeedant activity and high mortality in the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidae) induced by biostable insect kinin analogs SO PEPTIDES LA English DT Article DE Kinin analog; Antifeedant; Aphicide; Acyrthosiphon pisum; Insect; Gut; Myotropic; Diuresis; Peptidase resistant ID ANGIOTENSIN-CONVERTING ENZYME; TICK BOOPHILUS-MICROPLUS; MOSQUITO AEDES-AEGYPTI; NEUROPEPTIDE FAMILY; DIURETIC ACTIVITY; MUSCA-DOMESTICA; CONSENSUS CHEMISTRY; MALPIGHIAN TUBULES; ACHETA-DOMESTICUS; ARTIFICIAL DIET AB The insect kinins are multifunctional neuropeptides found in a variety of arthropod species, including the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidae). A series of biostable insect kinin analogs based on the shared C-terminal pentapeptide core region were fed in solutions of artificial diet to the pea aphid over a period of 3 days and evaluated for antifeedant and aphicidal activity. The analogs contained either alpha,alpha-disubstituted or beta-amino acids in key positions to enhance resistance to tissue-bound peptidases and retain activity in a number of insect kinin bioassays and/or on expressed receptors. Three of the biostable analogs demonstrated antifeedant activity, with a marked reduction in honeydew formation observed after 1 day, and very high mortality. In contrast, an unmodified, parent insect kinin and two other analogs containing some of the same structural components that promote biostability are inactive. The most active analog, double Aib analog K-Aib-1 ([Aib]FF[Aib]WGa), featured aphicidal activity calculated at an LC50 of 0.063 nmol/mu l (0.048 mu g/mu l) and an LT50 of 1.68 days, matching the potency of some commercially available aphicides. The mechanism of this activity has yet to be established. The aphicidal activity of the biostable insect kinin analogs may result from different potential mechanisms as disruption of digestive processes by interfering with gut motility patterns, digestive enzyme release, and/or with fluid cycling in the gut, and also nutrient transport across the gut itself; all processes shown to be regulated by the insect kinins in other insects. However the mechanism(s) is(are) not yet known. The active insect kinin analogs represent potential leads in the development of selective, environmentally friendly pest aphid control agents. (C) 2009 Elsevier Inc. All rights reserved. C1 [Smagghe, Guy; Mandian, Kamran] Univ Ghent, Fac Biosci Engn, Dept Crop Protect, Lab Agrozool, B-9000 Ghent, Belgium. [Zubrzak, Pawel; Nachman, Ronald J.] USDA, Areawide Pest Management Res, So Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Smagghe, G (reprint author), Univ Ghent, Fac Biosci Engn, Dept Crop Protect, Lab Agrozool, Coupure Links 653, B-9000 Ghent, Belgium. EM guy.smagghe@ugent.be; nachman@tamu.edu FU North Atlantic Treaty Organization (NATO) [LST.CLG.979226]; USDA/DOD DWFP Initiative [0500-32000-001-01R]; Fund of Scientific Research (FWO-Vlaanderen, Belgium) FX The authors wish to thank Allison Strey and Nan Pryor (USDA, College Station) for able technical assistance. We also acknowledge financial assistance from the North Atlantic Treaty Organization (NATO) (RJN) Collaborative Research Grant (#LST.CLG.979226), a grant from the USDA/DOD DWFP Initiative (#0500-32000-001-01R) (RJN), and support from the Fund of Scientific Research (FWO-Vlaanderen, Belgium) to GS. NR 52 TC 21 Z9 22 U1 0 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PD MAR PY 2010 VL 31 IS 3 SI SI BP 498 EP 505 DI 10.1016/j.peptides.2009.07.001 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 574MQ UT WOS:000275994100018 PM 19596392 ER PT J AU Kaufman, PE Nunez, SC Mann, RS Geden, CJ Scharf, ME AF Kaufman, Phillip E. Nunez, Sonia C. Mann, Rajinder S. Geden, Christopher J. Scharf, Michael E. TI Nicotinoid and pyrethroid insecticide resistance in houseflies (Diptera: Muscidae) collected from Florida dairies SO PEST MANAGEMENT SCIENCE LA English DT Article DE Insecta; resistance; imidacloprid; nithiazine; fly bait; pyrethroid; Musca domestica ID MUSCA-DOMESTICA L.; FLIES DIPTERA; SUSCEPTIBILITY AB BACKGROUND: The housefly, Musca domestica L., continues to be a major pest of confined livestock operations. Houseflies have developed resistance to most chemical classes, and new chemistries for use in animal agriculture are increasingly slow to emerge. Five adult housefly strains from four Florida dairy farms were evaluated for resistance to four insecticides (beta-cyfluthrin, permethrin, imidacloprid and nithiazine). RESULTS: Significant levels of tolerance were found in most field strains to all insecticides, and in some cases substantial resistance was apparent (as deduced from comparison with prior published results). At the LC(90) level, greater than 20-fold resistance was found in two of the fly strains for permethrin and one fly strain for imidacloprid. Beta-cyfluthrin LC(90) resistance ratios exceeded tenfold resistance in three fly strains. The relatively underutilized insecticide nithiazine had the lowest resistance ratios; however, fourfold LC(90) resistance was observed in one southern Florida fly strain. Farm insecticide use and its impact on resistance selection in Florida housefly populations are discussed. CONCLUSION: Housefly resistance to pyrethroids is widespread in Florida. Imidacloprid resistance is emerging, and tolerance was observed to both imidacloprid and nithiazine. If these insecticides are to retain efficacy, producer use must be restrained. (C) 2009 Society of Chemical Industry C1 [Kaufman, Phillip E.; Nunez, Sonia C.; Mann, Rajinder S.; Scharf, Michael E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Geden, Christopher J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Kaufman, PE (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM pkaufman@ufl.edu OI Kaufman, Phillip/0000-0001-6159-8358 FU Southeast Milk, Inc.; University of Florida Agricultural Experiment Station Federal Formula Funds [FLA-ENY-04598] FX The authors thank J Pitzer, L Wood and R Hammel for their assistance and the Florida dairy farmers who cooperated in this study. This research was supported by the Southeast Milk, Inc. Milk Check-Off grants program and in part by the University of Florida Agricultural Experiment Station Federal Formula Funds, project FLA-ENY-04598 (Cooperative State Research, Education and Extension Service, US Department of Agriculture). NR 14 TC 46 Z9 48 U1 0 U2 9 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAR PY 2010 VL 66 IS 3 BP 290 EP 294 DI 10.1002/ps.1872 PG 5 WC Agronomy; Entomology SC Agriculture; Entomology GA 562TZ UT WOS:000275079100008 PM 19904713 ER PT J AU Tansupo, P Suwannasom, P Luthria, DL Chanthai, S Ruangviriyachai, C AF Tansupo, Panadda Suwannasom, Pirom Luthria, Devanand L. Chanthai, Saksit Ruangviriyachai, Chalerm TI Optimised Separation Procedures for the Simultaneous Assay of Three Plant Hormones in Liquid Biofertilisers SO PHYTOCHEMICAL ANALYSIS LA English DT Article DE liquid biofertilisers; indole-3-acetic acid; giberellic acid; abscisic acid; sample preparation; HPLC analysis ID CHROMATOGRAPHY-MASS-SPECTROMETRY; ABSCISIC-ACID; GIBBERELLIC-ACID; CAPILLARY-ELECTROPHORESIS; ARABIDOPSIS-THALIANA; PHYTOHORMONES; METABOLITES; EXTRACTION; TRYPTOPHAN; OXIDASE AB Introduction - The overuse of petrochemical-based synthetic fertilisers has caused detrimental effects to soil, water supplies, foods and animal health. This, in addition to increased awareness of organic farming, has generated considerable interest in the evaluation of renewable biofertilisers. Objective - The three objectives of the current research were: (1) to evaluate and optimise a solid phase extraction procedure for extraction of three plant hormones, IAA, GA(3) and ABA from two model biofertilisers produced from coconut shells and pineapple peels; (2) to develop an HPLC analysis procedure for the simultaneous separation and quantification of three plant hormones (IAA, GA(3) and ABA); and (3) to evaluate the changes in three plant hormones levels at four different fermentation time periods and varying number of general bacteria, lactic acid bacteria and yeast. Result - An optimised procedure for sample preparation, separation and simultaneous analysis of three plant hormones [indole-3-acetic acid (IAA), gibberellic acid (GA(3)) and abscisic acid (ABA)] produced in liquid biofertilisers was developed. This method involves sample cleanup using a Sep-pack Oasis (R) MAX cartridge containing mixed-mode anion-exchange and reverse-phase sorbents that provided optimum recovery of 85.6, 91.9 and 94.3%, respectively, for the three hormones, IAA, GA(3), and ABA. Baseline separation of three hormones was achieved using mobile phase consisting of 1% acetic acid and acetonitrile (75:25, v/v) at pH 4.0. The amounts of hormones produced in liquid biofertilisers were influenced by fruit types, fermentation time and total number of general bacteria, lactic acid bacteria and yeasts. The quantities of three plant hormones produced during fermentation correlated well with the total number of microorganisms present in the liquid biofertilisers. Conclusion - A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA(3) and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the quantity of three hormones in other matrices. This assay procedure will aid in the development of liquid biofertilisers, a valuable alternative fertilisers to promote plant growth. This process will help farmers to reduce production cost and pollution problems. Copyright (C) 2009 John Wiley & Sons, Ltd. C1 [Tansupo, Panadda; Suwannasom, Pirom; Chanthai, Saksit; Ruangviriyachai, Chalerm] Khon Kaen Univ, Dept Chem, Khon Kaen 40002, Thailand. [Tansupo, Panadda; Suwannasom, Pirom; Chanthai, Saksit; Ruangviriyachai, Chalerm] Khon Kaen Univ, Fac Sci, Ctr Excellence Innovat Chem, Khon Kaen 40002, Thailand. [Luthria, Devanand L.] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20852 USA. RP Ruangviriyachai, C (reprint author), Khon Kaen Univ, Dept Chem, Khon Kaen 40002, Thailand. EM chal_ru@kku.ac.th FU Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education; Khon Kaen University FX Financial support from the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education is gratefully acknowledged. We would also like to thank Khon Kaen University for their research facility and grant. NR 33 TC 10 Z9 10 U1 1 U2 32 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0958-0344 J9 PHYTOCHEM ANALYSIS JI Phytochem. Anal. PD MAR-APR PY 2010 VL 21 IS 2 BP 157 EP 162 DI 10.1002/pca.1172 PG 6 WC Biochemical Research Methods; Plant Sciences; Chemistry, Analytical SC Biochemistry & Molecular Biology; Plant Sciences; Chemistry GA 561VH UT WOS:000275007400004 PM 19845039 ER PT J AU Hatfield, RD Marita, JM AF Hatfield, R. D. Marita, Jane M. TI Enzymatic processes involved in the incorporation of hydroxycinnamates into grass cell walls SO PHYTOCHEMISTRY REVIEWS LA English DT Article DE p-coumarates; Ferulates; Lignin; Acylation; Hydroxycinnamoyl transferases ID FERULATE CROSS-LINKS; P-COUMAROYL TRANSFERASE; ARABINOXYLAN OLIGOSACCHARIDES; PHENOLIC CONSTITUENTS; MAIZE INTERNODE; ACID-ESTERS; LIGNIN; POLYSACCHARIDES; FERULOYLATION; MODEL AB Many plant species have one or more types of acylation of cell wall polymers. Grasses (Poaceae family) are unique with abundant acylation of specific cell wall polymers by hydroxycinnamates. The most common hydroxycinnamates found in a wide range of grasses are ferulates (trans-4-hydroxy-3-methoxycinnamate) and p-coumarates (trans-4-hydroxycinnamate). These two hydroxycinnamates are synthesized by the phenylpropanoid pathway. Though structurally related, they seem to have different functional roles within the cell wall. Ferulates have been shown to have a critical role in cross-linking cell wall components; forming links between structural polysaccharides and links between structural polysaccharides and lignin. They are incorporated into the cell wall by distinctly different mechanisms. Ferulic acid is incorporated into cell walls as ester linked substituents on arabinoxylans. The exact role p-coumarates play in plant cell walls is unknown, but it has been shown that p-coumaric acid is ester-linked to monolignols and shuttled out to the wall to become incorporated into newly forming lignin polymers. Both processes require the activity of specific hydroxycinnamoyl transferases utilizing CoA derivatives to drive the transferase reactions. C1 [Hatfield, R. D.; Marita, Jane M.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Hatfield, RD (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM Ronald.hatfield@ars.usda.gov NR 47 TC 20 Z9 20 U1 0 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1568-7767 EI 1572-980X J9 PHYTOCHEM REV JI Phytochem. Rev. PD MAR PY 2010 VL 9 IS 1 SI SI BP 35 EP 45 DI 10.1007/s11101-010-9165-1 PG 11 WC Plant Sciences SC Plant Sciences GA 570FV UT WOS:000275658600003 ER PT J AU Almeida, NF Yan, SC Cai, RM Clarke, CR Morris, CE Schaad, NW Schuenzel, EL Lacy, GH Sun, XA Jones, JB Castillo, JA Bull, CT Leman, S Guttman, DS Setubal, JC Vinatzer, BA AF Almeida, Nalvo F. Yan, Shuangchun Cai, Rongman Clarke, Christopher R. Morris, Cindy E. Schaad, Norman W. Schuenzel, Erin L. Lacy, George H. Sun, Xiaoan Jones, Jeffrey B. Castillo, Jose A. Bull, Carolee T. Leman, Scotland Guttman, David S. Setubal, Joao C. Vinatzer, Boris A. TI PAMDB, A Multilocus Sequence Typing and Analysis Database and Website for Plant-Associated Microbes SO PHYTOPATHOLOGY LA English DT Article DE bacterial plant pathogens; plant disease diagnostics ID PATHOGEN PSEUDOMONAS-SYRINGAE; XANTHOMONAS-CITRI; BACTERIAL-BLIGHT; RECLASSIFICATION; RECOMBINATION; EVOLUTION; NOV.; RELATEDNESS; POPULATIONS; DIVERSITY AB Although there are adequate DNA sequence differences among plant-associated and plant-pathogenic bacteria to facilitate molecular approaches for their identification, identification at a taxonomic level that is predictive of their phenotype is a challenge. The problem is the absence of a taxonomy that describes genetic variation at a biologically relevant resolution and of a database containing reference strains for comparison. Moreover, molecular evolution, population genetics, ecology, and epidemiology of many plant-pathogenic and plant-associated bacteria are still poorly understood. To address these challenges, a database with web interface was specifically designed for plant-associated and plant-pathogenic microorganisms. The Plant-Associated Microbes Database (PAMDB) comprises, thus far, data from multilocus sequence typing and analysis (MLST/MLSA) studies of Acidovorax citrulli, Pseudomonas syringae, Ralstonia solanacearum, and Xanthomonas spp. Using data deposited in PAMDB, a robust phylogeny of Xanthomonas axonopodis and related bacteria has been inferred, and the diversity existing in the Xanthomonas genus and in described Xanthomonas spp. has been compared with the diversity in P. syringae and R. solanacearum. Moreover, we show how PAMDB makes it easy to distinguish between different pathogens that cause almost identical diseases. The scalable design of PAMDB will make it easy to add more plant pathogens in the future. C1 [Almeida, Nalvo F.; Yan, Shuangchun; Cai, Rongman; Clarke, Christopher R.; Lacy, George H.; Vinatzer, Boris A.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Almeida, Nalvo F.; Setubal, Joao C.] Virginia Tech, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA. [Almeida, Nalvo F.] Univ Fed Mato Grosso do Sul, Dept Comp & Stat, Campo Grande, Brazil. [Morris, Cindy E.] INRA, Unite Pathol Vegetale, UR407, F-84140 Montfavet, France. [Schaad, Norman W.; Schuenzel, Erin L.] USDA ARS, Ft Detrick, MD 21702 USA. [Sun, Xiaoan] Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [Jones, Jeffrey B.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Castillo, Jose A.] PROINPA Fdn, Cochabamba, Bolivia. [Bull, Carolee T.] USDA ARS, Salinas, CA 93905 USA. [Leman, Scotland] Virginia Tech, Dept Stat, Blacksburg, VA 24061 USA. [Guttman, David S.] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON M5S 3B2, Canada. [Setubal, Joao C.] Virginia Tech, Dept Comp Sci, Blacksburg, VA 24061 USA. RP Vinatzer, BA (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM vinatzer@vt.edu RI Almeida, Nalvo/B-5856-2012; Setubal, Joao/C-7305-2012; Oncogenomica, Inct/H-9999-2013; Cai, Rongman/C-5998-2015; OI Setubal, Joao/0000-0001-9174-2816; Cai, Rongman/0000-0003-2404-4852; Yan, Shuangchun/0000-0002-9924-283X; Almeida, Nalvo/0000-0001-5615-1746 FU NSF [IOS 0746501]; Virginia Tech; Virginia Tech ASPIRES; CNPq [200447/2007-6] FX Research in the Vinatzer lab was funded by the NSF (Award IOS 0746501), Virginia Tech start-up funds, and a Virginia Tech ASPIRES grant. N. F. Almeida was partly supported by the CNPq grant 200447/2007-6. We thank the Virginia Tech undergraduate students T. Wiseman, N. Long, and L. Williams for their help with DNA extractions, PCR, and DNA sequence analysis. NR 48 TC 68 Z9 70 U1 4 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2010 VL 100 IS 3 BP 208 EP 215 DI 10.1094/PHYTO-100-3-0208 PG 8 WC Plant Sciences SC Plant Sciences GA 551WV UT WOS:000274244800001 PM 20128693 ER PT J AU Tatineni, S Graybosch, RA Hein, GL Wegulo, SN French, R AF Tatineni, Satyanarayana Graybosch, Robert A. Hein, Gary L. Wegulo, Stephen N. French, Roy TI Wheat Cultivar-Specific Disease Synergism and Alteration of Virus Accumulation During Co-Infection with Wheat streak mosaic virus and Triticum mosaic virus SO PHYTOPATHOLOGY LA English DT Article ID TIME RT-PCR; REVERSE TRANSCRIPTION-PCR; CHLOROTIC-MOTTLE-VIRUS; CITRUS-TRISTEZA-VIRUS; FAMILY POTYVIRIDAE; MIXED INFECTIONS; HC-PRO; MAIZE; MITE; QUANTIFICATION AB Triticum mosaic virus (TriMV), the type member of the newly proposed Poacevirus genus, and Wheat streak mosaic virus (WSMV), the type member of Tritimovirus genus of the family Potyviridae, infect wheat naturally in the Great Plains and are transmitted by wheat curl mites. In this study, we examined the ability of these viruses to infect selected cereal hosts, and found several differential hosts between TriMV and WSMV. Additionally, we examined the interaction between WSMV and TriMV in three wheat cultivars at two temperature regimens (19 and 20 to 26 degrees C), and quantified the virus concentration in single and double infections by real-time reverse-transcription polymerase chain reaction. Double infections in wheat cvs. Arapahoe and Tomahawk at both temperature regimens induced disease synergism with severe leaf deformation, bleaching, and stunting, with a 2.2- to 7.4-fold increase in accumulation of both viruses over single infections at 14 days postinoculation (dpi). However, at 28 dpi, in double infections at 20 to 26 degrees C, TriMV concentration was increased by 1.4- to 1.8-fold in Arapahoe and Tomahawk but WSMV concentration was decreased to 0.5-fold. WSMV or TriMV replicated poorly in Mace at 19 degrees C with no synergistic interaction whereas both viruses accumulated at moderate levels at 20 to 26 degrees C and induced mild to moderate disease synergism in doubly infected Mace compared with Arapahoe and Tomahawk. Co-infections in Mace at 20 to 26 degrees C caused increased TriMV accumulation at 14 and 28 dpi by 2.6- and 1.4-fold and WSMV accumulated at 0.5- and 1.6-fold over single infections, respectively. Our data suggest that WSMV and TriMV induced cultivar-specific disease synergism in Arapahoe, Tomahawk, and Mace, and these findings could have several implications for management of wheat viruses in the Great Plains. C1 [Tatineni, Satyanarayana; Graybosch, Robert A.; French, Roy] USDA ARS, Washington, DC 20250 USA. [Tatineni, Satyanarayana; Wegulo, Stephen N.; French, Roy] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Graybosch, Robert A.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Hein, Gary L.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. RP Tatineni, S (reprint author), USDA ARS, Washington, DC 20250 USA. EM Satyanarayana.tatineni@ars.usda.gov OI Wegulo, Stephen/0000-0002-4435-6055 NR 31 TC 49 Z9 49 U1 3 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2010 VL 100 IS 3 BP 230 EP 238 DI 10.1094/PHYTO-100-3-0230 PG 9 WC Plant Sciences SC Plant Sciences GA 551WV UT WOS:000274244800004 PM 20128696 ER PT J AU Zhang, MQ Duan, YP Zhou, LJ Turechek, WW Stover, E Powell, CA AF Zhang, Muqing Duan, Yongping Zhou, Lijuan Turechek, William W. Stover, Ed Powell, Charles A. TI Screening Molecules for Control of Citrus Huanglongbing Using an Optimized Regeneration System for 'Candidatus Liberibacter asiaticus'-Infected Periwinkle (Catharanthus roseus) Cuttings SO PHYTOPATHOLOGY LA English DT Article ID CA. L. AMERICANUS; SAO-PAULO STATE; REAL-TIME PCR; GREENING DISEASE; SWEET ORANGE; ROOT DEVELOPMENT; UNIFORM DESIGN; UNITED-STATES; FIRE BLIGHT; TRANSMISSION AB Citrus huanglongbing is one of the most destructive diseases of citrus worldwide. The disease is associated with three different species of 'Candidatus Liberibacter', of which 'Ca. L. asiaticus' is the most widely distributed. An optimized system using 'Ca. L. asiaticus'-infected periwinkle cuttings was developed to screen chemical compounds effective for controlling the bacterial population while simultaneously assessing their phytotoxicity. The optimal regeneration conditions were determined to be the use of vermiculite as a growth medium for the cuttings, and a fertilization routine using half-strength Murashige and Tucker medium supplemented with both naphthalene acetic acid (4 mu g/ml) and indole-3-butyric acid (4 mu g/ml). This system allowed a plant regeneration rate of 60.6% for 'Ca. L. asiaticus'-infected cuttings in contrast to the < 1% regeneration rate with water alone. Two chemical agents, penicillin G sodium and 2,2-dibromo-3-nitrilopropionamide (DBNPA), were found to be effective at eliminating or suppressing the 'Ca. L. asiaticus' bacterium in this periwinkle regeneration system. When treated with penicillin G sodium at 50 mu g/ml, all plants regenerated from 'Ca. L. asiaticus'-infected cuttings were 'Ca. L. asiaticus' negative as determined by both nested polymerase chain reaction (PCR) and quantitative real-time PCR. In addition, DBNPA was also able to significantly reduce the percentage of 'Ca. L. asiaticus'-positive plants and the titer of the 'Ca. L. asiaticus' bacterium at 200 mu l/liter. C1 [Zhang, Muqing; Powell, Charles A.] Univ Florida, IFAS IRREC, Ft Pierce, FL 34945 USA. [Duan, Yongping; Zhou, Lijuan; Turechek, William W.; Stover, Ed] USDA ARS, USHRL, Ft Pierce, FL 34945 USA. RP Powell, CA (reprint author), Univ Florida, IFAS IRREC, Ft Pierce, FL 34945 USA. EM capowell@ufl.edu FU Florida Citrus Production Research Advisory Council (FCPRAC) FX We thank C. Vahling for critical review on the manuscript. This research was supported by grants from the Florida Citrus Production Research Advisory Council (FCPRAC). NR 47 TC 16 Z9 19 U1 3 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2010 VL 100 IS 3 BP 239 EP 245 DI 10.1094/PHYTO-100-3-0239 PG 7 WC Plant Sciences SC Plant Sciences GA 551WV UT WOS:000274244800005 PM 20128697 ER PT J AU Springsteen, A Loya, W Liebig, M Hendrickson, J AF Springsteen, Anna Loya, Wendy Liebig, Mark Hendrickson, John TI Soil carbon and nitrogen across a chronosequence of woody plant expansion in North Dakota SO PLANT AND SOIL LA English DT Article DE Microbial biomass; Coarse particulate organic matter; Grasslands ID ECOSYSTEM CARBON; MESIC GRASSLAND; ORGANIC-MATTER; JUNIPERUS-VIRGINIANA; SUBTROPICAL SAVANNA; SHRUB ENCROACHMENT; GREAT-PLAINS; INVASION; DYNAMICS; CONSEQUENCES AB Woody plant expansion has been documented on grasslands worldwide as a result of overgrazing and fire suppression, but changes in ecosystem structure and function accompanying this phenomenon have yet to be extensively studied in the temperate semi-arid grasslands of North America. The primary objectives of this study were to determine the influence of woody plant expansion on soil carbon (C), soil nitrogen (N), and roots to a depth of 15 cm along a 42-year (1963-2005) chronosequence encompassing grassland, woodland, and transition zones in a northern Great Plains grassland. From these data, we also estimated ecosystem-level soil C and N changes associated with woody plant expansion in the top 0-15 cm of soil. We found total soil C increased across the chronosequence from grassland (5,070 +/- 250 g C m(-2)) to woodland (6,370 +/- 390 g C m(-2)) (P < 0.05) at 0-15 cm soil depth. Total soil N also increased from grassland to woodland (425 +/- 16 to 556 +/- 30 g N m(-2)) (P < 0.05) at 0-15 cm soil depth. Coarse particulate organic matter C and N increased from grassland to woodland (940 +/- 100 to 598 +/- 35 g C m(-2), 70 +/- 10 to 35 +/- 1 g N m(-2)) at 0-5 cm soil depth. At the ecosystem-level, we estimate C and N accumulations at 0-15 cm soil depth are occurring at a rate of 18 and 1.7 g m(-2) year(-1), respectively. Results of this study suggest soil resources, namely soil C and N, in the northern Great Plains are changing following woody plant expansion. C1 [Springsteen, Anna] Univ N Dakota, Grand Forks, ND 58201 USA. [Loya, Wendy] Wilderness Soc, Anchorage, AK 99501 USA. [Liebig, Mark; Hendrickson, John] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Springsteen, A (reprint author), Univ Alaska, Scenarios Network Alaska Planning, 341 ONeill Bldg,POB 757200, Fairbanks, AK 99775 USA. EM alspringsteen@alaska.edu FU USDA-ARS Northern Great Plains Research Laboratory; USDA-ARS; Upper Midwest Aerospace Consortium FX We thank Dr. Ofer Beeri for sharing his analysis of shrub expansion which was the basis for this study. Dr. Rod Hanley and Dr. Brett Goodwin provided additional guidance and help in statistical matters. Support from personnel at the USDA-ARS Northern Great Plains Research Laboratory is gratefully acknowledged, particularly Mary Kay Tokach for her assistance with vegetation identification. This work was funded through a grant by the Northern Great Plains Center for People and the Environment and a Specific Cooperative Agreement between USDA-ARS and the Upper Midwest Aerospace Consortium. NR 50 TC 15 Z9 16 U1 1 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X J9 PLANT SOIL JI Plant Soil PD MAR PY 2010 VL 328 IS 1-2 BP 369 EP 379 DI 10.1007/s11104-009-0117-8 PG 11 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA 558GZ UT WOS:000274730400030 ER PT J AU Cook, D Rimando, AM Clemente, TE Schroder, J Dayan, FE Nanayakkara, NPD Pan, ZQ Noonan, BP Fishbein, M Abe, I Duke, SO Baerson, SR AF Cook, Daniel Rimando, Agnes M. Clemente, Thomas E. Schroeder, Joachim Dayan, Franck E. Nanayakkara, N. P. Dhammika Pan, Zhiqiang Noonan, Brice P. Fishbein, Mark Abe, Ikuro Duke, Stephen O. Baerson, Scott R. TI Alkylresorcinol Synthases Expressed in Sorghum bicolor Root Hairs Play an Essential Role in the Biosynthesis of the Allelopathic Benzoquinone Sorgoleone SO PLANT CELL LA English DT Article ID III POLYKETIDE SYNTHASE; CHAIN-LENGTH CONTROL; CHALCONE SYNTHASE; RESORCINOLIC LIPIDS; PENTAKETIDE CHROMONE; STILBENE SYNTHASES; NEUROSPORA-CRASSA; ALOE-ARBORESCENS; STRIGA-ASIATICA; SWISS-MODEL AB Sorghum bicolor is considered to be an allelopathic crop species, producing phytotoxins such as the lipid benzoquinone sorgoleone, which likely accounts for many of the allelopathic properties of Sorghum spp. Current evidence suggests that sorgoleone biosynthesis occurs exclusively in root hair cells and involves the production of an alkylresorcinolic intermediate (5-[(Z, Z)-8',11',14'-pentadecatrienyl]resorcinol) derived from an unusual 16:3 Delta(9,12,15) fatty acyl-CoA starter unit. This led to the suggestion of the involvement of one or more alkylresorcinol synthases (ARSs), type III polyketide synthases (PKSs) that produce 5-alkylresorcinols using medium to long-chain fatty acyl-CoA starter units via iterative condensations with malonyl-CoA. In an effort to characterize the enzymes responsible for the biosynthesis of the pentadecyl resorcinol intermediate, a previously described expressed sequence tag database prepared from isolated S. bicolor (genotype BTx623) root hairs was first mined for all PKS-like sequences. Quantitative real-time RT-PCR analyses revealed that three of these sequences were preferentially expressed in root hairs, two of which (designated ARS1 and ARS2) were found to encode ARS enzymes capable of accepting a variety of fatty acyl-CoA starter units in recombinant enzyme studies. Furthermore, RNA interference experiments directed against ARS1 and ARS2 resulted in the generation of multiple independent transformant events exhibiting dramatically reduced sorgoleone levels. Thus, both ARS1 and ARS2 are likely to participate in the biosynthesis of sorgoleone in planta. The sequences of ARS1 and ARS2 were also used to identify several rice (Oryza sativa) genes encoding ARSs, which are likely involved in the production of defense-related alkylresorcinols. C1 [Cook, Daniel; Rimando, Agnes M.; Dayan, Franck E.; Pan, Zhiqiang; Duke, Stephen O.; Baerson, Scott R.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Clemente, Thomas E.] Univ Nebraska, Ctr Biotechnol, Lincoln, NE 68588 USA. [Schroeder, Joachim] Univ Freiburg, Inst Biol 2, D-79104 Freiburg, Germany. [Nanayakkara, N. P. Dhammika] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Noonan, Brice P.] Univ Mississippi, Dept Biol, University, MS 38677 USA. [Fishbein, Mark] Oklahoma State Univ, Dept Bot, Stillwater, OK 74078 USA. [Abe, Ikuro] Univ Tokyo, Grad Sch Pharmaceut Sci, Tokyo 1130033, Japan. RP Baerson, SR (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. EM scott.baerson@ars.usda.gov RI Abe, Ikuro/E-4449-2010; Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 NR 79 TC 31 Z9 33 U1 2 U2 16 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD MAR PY 2010 VL 22 IS 3 BP 867 EP 887 DI 10.1105/tpc.109.072397 PG 21 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 587IJ UT WOS:000276983900026 PM 20348430 ER PT J AU Schoonmaker, AL Hacke, UG Landhausser, SM Lieffers, VJ Tyree, MT AF Schoonmaker, Amanda L. Hacke, Uwe G. Landhaeusser, Simon M. Lieffers, Victor J. Tyree, Melvin T. TI Hydraulic acclimation to shading in boreal conifers of varying shade tolerance SO PLANT CELL AND ENVIRONMENT LA English DT Article DE cavitation; conductivity; pine; spruce; water relations; wood anatomy ID FAGUS-SYLVATICA L; PIT MEMBRANES; DOUGLAS-FIR; WESTERN HEMLOCK; LODGEPOLE PINE; PICEA-ABIES; XYLEM; CAVITATION; EMBOLISM; TREES AB The purpose of this study was to determine how shading affects the hydraulic and wood-anatomical characteristics of four boreal conifers (Pinus banksiana, Pinus contorta, Picea glauca and Picea mariana) that differ in shade tolerance. Plants were grown in an open field and under a deciduous-dominated overstory for 6 years. Sapwood- and leaf-area specific conductivity, vulnerability curves, and anatomical measurements (light and scanning electron microscopy) were made on leading shoots from six to nine trees of each treatment combination. There was no difference in sapwood-area specific conductivity between open-grown and understory conifers, although two of four species had larger tracheid diameters in the open. Shaded conifers appeared to compensate for small diameter tracheids by changes in pit membrane structure. Scanning electron microscopy revealed that understory conifers had thinner margo strands, greater maximum pore size in the margo, and more torus extensions. All of these trends may contribute to inadequate sealing of the torus. This is supported by the fact that all species showed increased vulnerability to cavitation when grown in the understory. Although evaporative demand in an understory environment is low, a rapid change into fully exposed conditions could be detrimental for shaded conifers. C1 [Schoonmaker, Amanda L.; Hacke, Uwe G.; Landhaeusser, Simon M.; Lieffers, Victor J.; Tyree, Melvin T.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. [Tyree, Melvin T.] US Forest Serv, No Expt Stn, S Burlington, VT 05403 USA. RP Schoonmaker, AL (reprint author), Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. EM schoonma@ualberta.ca RI Hacke, Uwe/M-7719-2015; Landhausser, Simon/F-9910-2016; OI Hacke, Uwe/0000-0001-5983-3582; Landhausser, Simon/0000-0002-4466-1607; Lieffers, Victor/0000-0001-6939-4093 FU NSERC PGSM; CGSD; Alberta Ingenuity Scholarship; Canada Research Chair program; Canada Foundation for Innovation FX We gratefully acknowledge field and lab assistance from Jennifer Langhorst, Jessica Snedden, Kevin Renkema, Dominique Deshaies, Kelci Mohr, Caroline Lecoutier, Kristine Dahl and Kim Stang. We thank George Braybrook and De-Ann Rollings for assistance with the SEM work. An NSERC PGSM and CGSD and Alberta Ingenuity Scholarship to ALS, NSERC and Mixedwood Management Association grants to VJL and support from the Canada Research Chair program and the Canada Foundation for Innovation to UGH were all greatly appreciated. NR 41 TC 22 Z9 23 U1 1 U2 31 PU WILEY-BLACKWELL PUBLISHING, INC PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0140-7791 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD MAR PY 2010 VL 33 IS 3 BP 382 EP 393 DI 10.1111/j.1365-3040.2009.02088.x PG 12 WC Plant Sciences SC Plant Sciences GA 554CG UT WOS:000274412500007 PM 19968826 ER PT J AU Stuteville, DL Graves, WL Dixon, LJ Castlebury, LA Minnis, AM AF Stuteville, D. L. Graves, W. L. Dixon, L. J. Castlebury, L. A. Minnis, A. M. TI Uromyces ciceris-arietini, the Cause of Chickpea Rust: New Hosts in the Trifolieae, Fabaceae SO PLANT DISEASE LA English DT Article ID RIBOSOMAL DNA; UNITED-STATES; FUNGI; COLLECTION; LEGUMES; FAMILY AB Uromyces ciceris-arietini has been reported on Cicer arietinum (chickpea) and Medicago polyceratia. Plants of Medicago polymorpha in Riverside and San Diego, CA were collected with severe rust caused by U. ciceris-arietini. To confirm the identification and potential new host range, a monouredinial isolate of U. ciceris-arietini from M. polymorpha was inoculated on eight accessions each of C. arietinum and M. polyceratia. All plants showed symptoms of the disease. Consequently, a range of fabaceous hosts were evaluated for their reaction to U. ciceris-arietini. New hosts for U. ciceris-arietini included 29 species of Medicago, specifically M. arabica, M. blancheana, M. ciliaris, M. constricta, M. coronata, M. doliata, M. granadensis, M. intertexta, M. italica, M. laciniata, M. lanigera, M. lesinsii, M. lupulina, M. minima, M. murex, M. muricoleptis, M. orbicularis, M. praecox, M. radiata, M. rigidula, M. rotata, M. rugosa, M. sativa, M. sauvagei, M. scutellata, M. soleirolii, M. tenoreana, M. truncatula, and M. varia, and three species of Melilotus, specifically M. italicus, M. speciosus, and M. spicatus. This isolate of U. ciceris-arietini produced no symptoms oil plants in the 33 accessions tested in the genera Anthyllis, Astragalus, Lotus, and Lupinus. DNA sequences are provided to aid in the identification of this pathogen. C1 [Dixon, L. J.; Castlebury, L. A.; Minnis, A. M.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Graves, W. L.] Univ Calif, Cooperat Extens Serv, Blythe, CA 92225 USA. [Stuteville, D. L.] Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Manhattan, KS 66502 USA. RP Minnis, AM (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Rm 304,B011A,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Drew.Minnis@ars.usda.gov NR 29 TC 2 Z9 2 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2010 VL 94 IS 3 BP 293 EP 297 DI 10.1094/PDIS-94-3-0293 PG 5 WC Plant Sciences SC Plant Sciences GA 558LQ UT WOS:000274744300002 ER PT J AU Ling, KS AF Ling, Kai-Shu TI Effectiveness of Chemo- and Thermotherapeutic Treatments on Pepino mosaic virus in Tomato Seed SO PLANT DISEASE LA English DT Article ID GREENHOUSE TOMATOES; GENOMIC RNA; ISOLATE; TRANSMISSION; SEQUENCE; POPULATION; DISTINCT; PERU AB Pepino mosaic virus (PepMV) is a seedborne virus of importance in greenhouse tomato. The ease of mechanical transmission of PepMV from contaminated tomato seed to seedlings makes commercial tomato seed a potential source of initial virus inoculum. The objective of this study was to evaluate the effectiveness of chemo- and thermotherapeutic treatments on PepMV using seed from a commercial hybrid tomato seed lot. The effect of various seed treatments was evaluated through bioassay with mechanical inoculation of the treated seed extract on indicator plants (Nicotiana benthamiana). Three commonly used seed-treatment chemicals and two thermotreatment methods were evaluated. The most effective chemical was the commercial bleach solution (0.5 and 1.0% sodium hypochlorite), followed by trisodium phosphate. On the other hand, hydrochloric acid treatments were not effective. Under the stated chemotherapeutic treatment conditions, the rate of seed germination was not significantly affected. With thermotherapeutic treatments, although hot water soaking for 2 h at 55 degrees C deactivated virus infectivity, it also resulted in a detrimental effect on seed germination. However, treatment with dry-heat baking (72 or 80 degrees C for 48 to 72 h) was effective in reducing PepMV infection with minimum impact on seed germination. C1 USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. EM kai.ling@ars.usda.gov NR 20 TC 6 Z9 6 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2010 VL 94 IS 3 BP 325 EP 328 DI 10.1094/PDIS-94-3-0325 PG 4 WC Plant Sciences SC Plant Sciences GA 558LQ UT WOS:000274744300007 ER PT J AU Slaminko, TL Bowen, CR Hartman, GL AF Slaminko, T. L. Bowen, C. R. Hartman, G. L. TI Multi-Year Evaluation of Commercial Soybean Cultivars for Resistance to Phytophthora sojae SO PLANT DISEASE LA English DT Article ID FIELDS; RACES; OHIO AB Phytophthora sojae causes damping-off, root rot, and stem rot of soybean, particularly in poorly drained soils. Soybean cultivar resistance is one of the primary methods to control this disease, with Rps1c, Rps1k, and Rps1a being the most commonly used genes. The Varietal Information Program for Soybeans (VIPS) at the University of Illinois evaluates soybean cultivars for resistance to a number of diseases including Phytophthora root rot (PRR). The objectives of this research were to evaluate PRR resistance among commercial cultivars or advanced lines, and to compare these results with the information on PRR resistance provided by the company that entered the cultivar in VIPS. Each year from 2004 to 2008, between 600 and 900 cultivars were evaluated for resistance to either race 17 or 26 of P. sojae using the hypocotyl inoculation method. P. sojae single resistance genes were reported in 1,808 or 51% of the entries based on company information. Of these, the most commonly reported resistance genes were Rps1c (50%), Rps1k (40%), and Rps1a (10%). To a much smaller degree, companies reported using Rps3a (0.3%), Rps1b (0.2%), and Rps7 (0.2%). For the duration of the 5-year testing period, almost half of the cultivars (46%) were entered in VIPS with no reported resistance genes, and only nine out of a total of 3,533 entries (less than 0.3%) reported a stacked combination of resistance genes. Agreement between company-reported genes and any resistance found in the VIPS PRR evaluation was highest for those cultivars claiming to have Rps1c (90%) and Rps1k (83%), followed by Rps1a (70%). On average. 54% of the cultivars submitted to VIPS each year were new, reflecting the rapid development and turnover of soybean cultivars provided by the soybean seed companies. C1 [Bowen, C. R.; Hartman, G. L.] USDA ARS, Urbana, IL 61801 USA. [Slaminko, T. L.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), USDA ARS, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU Illinois Soybean Association FX We thank the Illinois Soybean Association for support of this research through funding of VIPS, the University of Illinois Soybean Variety Testing Program. We also thank E. Grunden and D. Malvick for technical assistance, and T. Herman for editorial review. NR 11 TC 8 Z9 10 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2010 VL 94 IS 3 BP 368 EP 371 DI 10.1094/PDIS-94-3-0368 PG 4 WC Plant Sciences SC Plant Sciences GA 558LQ UT WOS:000274744300015 ER PT J AU Robertson, NL Brown, KL AF Robertson, N. L. Brown, K. L. TI First Report of Bean yellow mosaic virus in Alaska from Clover (Trifolium spp.). SO PLANT DISEASE LA English DT News Item C1 [Robertson, N. L.; Brown, K. L.] USDA ARS, Subarct Agr Res Unit, Palmer, AK 99645 USA. RP Robertson, NL (reprint author), USDA ARS, Subarct Agr Res Unit, Palmer, AK 99645 USA. NR 3 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2010 VL 94 IS 3 BP 372 EP 372 DI 10.1094/PDIS-94-3-0372A PG 1 WC Plant Sciences SC Plant Sciences GA 558LQ UT WOS:000274744300016 ER PT J AU Brown, JK Rehman, M Rogan, D Martin, RR Idris, AM AF Brown, J. K. Rehman, M. Rogan, D. Martin, R. R. Idris, A. M. TI First Report of "Candidatus Liberibacter psyllaurous" (synonym "Ca. L. solanacearum") Associated with 'Tomato Vein-Greening' and 'Tomato Psyllid Yellows' Diseases in Commercial Greenhouses in Arizona. SO PLANT DISEASE LA English DT News Item C1 [Brown, J. K.; Rogan, D.; Idris, A. M.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Rehman, M.] Hazara Univ, Dept Microbiol, Mansehra, Pakistan. [Martin, R. R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. RP Brown, JK (reprint author), Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. NR 4 TC 11 Z9 15 U1 1 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2010 VL 94 IS 3 BP 376 EP 376 DI 10.1094/PDIS-94-3-0376B PG 1 WC Plant Sciences SC Plant Sciences GA 558LQ UT WOS:000274744300028 ER PT J AU Medina, E Cuevas, E Lugo, AE AF Medina, Ernesto Cuevas, Elvira Lugo, Ariel E. TI Nutrient relations of dwarf Rhizophora mangle L. mangroves on peat in eastern Puerto Rico SO PLANT ECOLOGY LA English DT Article DE Mangroves; Nutrient resorption; Salinity; C-13; N-15 ID VS. PHOSPHORUS LIMITATION; RED MANGROVE; SOUTH FLORIDA; RIVER ESTUARY; NITROGEN; FOREST; SOIL; GROWTH; LEAF; PHOTOSYNTHESIS AB Dwarf mangroves on peat substrate growing in eastern Puerto Rico (Los Machos, Ceiba State Forest) were analyzed for element concentration, leaf sap osmolality, and isotopic signatures of C and N in leaves and substrate. Mangrove communities behind the fringe presented poor structural development with maximum height below 1.5 m, lacked a main stem, and produced horizontal stems from which rhizophores developed. This growth form departs from other dwarf mangrove sites in Belize, Panama, and Florida. The dwarf mangroves were not stressed by salinity but by the low P availability reflected in low P concentrations in adult and senescent leaves. Low P availability was associated with reduced remobilization of N and accumulation of K in senescent leaves, contrasting with the behavior of this cation in terrestrial plants. Remobilization of N and P before leaf abscission on a weight basis indicated complete resorption of these nutrients. On an area basis, resorption was complete for P but not for N. Sulfur accumulated markedly with leaf age, reaching values up to 400%, compared with relatively modest accumulation of Na (40%) in the same leaves. This suggests a more effective rejection of Na than sulfate at the root level. Dwarf mangrove leaves had more positive delta C-13 values, which were not related to salinity, but possibly to drought during the dry season due to reduced flooding, and/or reduced hydraulic conductance under P limitation. Negative leaf delta N-15 values were associated with low leaf P concentrations. Comparison with other R. mangle communities showed that P concentration in adult leaves below 13 mmol kg(-1) is associated with negative delta N-15 values, whereas leaves with P concentrations above 30 mmol kg(-1) in non-polluted environments had positive delta N-15 values. C1 [Medina, Ernesto] Inst Venezolano Invest Cient, Ctr Ecol, Caracas 1020A, Venezuela. [Medina, Ernesto; Lugo, Ariel E.] US Forest Serv, Int Inst Trop Forestry, USDA, Jardin Bot, San Juan, PR 00926 USA. [Cuevas, Elvira] Univ Puerto Rico, Dept Biol, San Juan, PR 00931 USA. RP Medina, E (reprint author), Inst Venezolano Invest Cient, Ctr Ecol, Aptdo 21827, Caracas 1020A, Venezuela. EM medinage@gmail.com NR 40 TC 14 Z9 16 U1 6 U2 42 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD MAR PY 2010 VL 207 IS 1 BP 13 EP 24 DI 10.1007/s11258-009-9650-z PG 12 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 551LT UT WOS:000274211800002 ER PT J AU Mikstacka, R Rimando, AM Ignatowicz, E AF Mikstacka, Renata Rimando, Agnes M. Ignatowicz, Ewa TI Antioxidant Effect of trans-Resveratrol, Pterostilbene, Quercetin and Their Combinations in Human Erythrocytes In Vitro SO PLANT FOODS FOR HUMAN NUTRITION LA English DT Article DE Lipid peroxidation; Hemolysis; Combination effect; Resveratrol; Pterostilbene; Quercetin ID RED WINE; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; VITIS-VINIFERA; CELLS; FLAVONOIDS; MEMBRANE; ACID; PROLIFERATION AB There is evidence that a diet rich in fruit and vegetables may reduce the risk of cancer and other degenerative diseases. However, potential health impact of bioactive phytochemicals is limited by their low amount and relatively poor bioavailability. It has been suggested that the health benefits associated with fruit and red wine consumption could be due to the whole antioxidant pool of the diet microcomponents. In this study, the antioxidant activities of trans-resveratrol, pterostilbene and quercetin, and the effect of their combination were investigated in human erythrocytes in vitro. H(2)O(2)-induced lipid peroxidation was assessed by measuring the amount of thiobarbituric acid reactive species. Quercetin and pterostilbene protected erythrocyte membranes against lipid peroxidation (IC(50) values = 64 +/- 8.7 A mu M and 44.5 A +/- 7.8 A mu M, respectively). Resveratrol was significantly less effective. However, the three compounds protected the erythocytes against hemolysis and GSH (reduced glutathione) depletion to the same extent. Combinations consisting of two compounds (molar ratio 1:1) influenced lipid peroxidation in a concentration-dependent manner. At lower concentrations, resveratrol with quercetin or pterostilbene inhibited synergistically the oxidative injury of membrane lipids At higher concentrations, an additive effect was observed. These protective effects may partially explain the health benefit of these bioactive microcomponents when together in the diet. C1 [Mikstacka, Renata] Karol Marcinkowski Univ Med Sci, Dept Chem Technol Drugs, PL-60780 Poznan, Poland. [Rimando, Agnes M.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Ignatowicz, Ewa] Karol Marcinkowski Univ Med Sci, Dept Pharmaceut Biochem, PL-60781 Poznan, Poland. RP Mikstacka, R (reprint author), Karol Marcinkowski Univ Med Sci, Dept Chem Technol Drugs, Grunwaldzka 6, PL-60780 Poznan, Poland. EM rmikstac@ump.edu.pl NR 34 TC 72 Z9 76 U1 2 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-9668 J9 PLANT FOOD HUM NUTR JI Plant Food Hum. Nutr. PD MAR PY 2010 VL 65 IS 1 BP 57 EP 63 DI 10.1007/s11130-010-0154-8 PG 7 WC Plant Sciences; Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Plant Sciences; Chemistry; Food Science & Technology; Nutrition & Dietetics GA 571KF UT WOS:000275750000009 PM 20108046 ER PT J AU Maron, LG Pineros, MA Guimaraes, CT Magalhaes, JV Pleiman, JK Mao, CZ Shaff, J Belicuas, SNJ Kochian, LV AF Maron, Lyza G. Pineros, Miguel A. Guimaraes, Claudia T. Magalhaes, Jurandir V. Pleiman, Jennifer K. Mao, Chuanzao Shaff, Jon Belicuas, Silvia N. J. Kochian, Leon V. TI Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize SO PLANT JOURNAL LA English DT Article DE aluminum; tolerance; multi-drug and toxic compound extrusion (MATE); transporter; maize; organic acids ID ORGANIC-ACID EXUDATION; MALATE TRANSPORTERS; CITRATE TRANSPORTER; VACUOLAR TRANSPORT; ACTIVATED CITRATE; GENE-EXPRESSION; MULTIPLE GENES; TROPICAL MAIZE; SOIL ACIDITY; ARABIDOPSIS AB P>Crop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world's arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL found on chromosome 6. However, ZmMATE2 most likely does not encode a citrate transporter, and could be involved in a novel Al tolerance mechanism. C1 [Maron, Lyza G.; Pineros, Miguel A.; Pleiman, Jennifer K.; Mao, Chuanzao; Shaff, Jon; Kochian, Leon V.] Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Guimaraes, Claudia T.; Magalhaes, Jurandir V.; Belicuas, Silvia N. J.] EMBRAPA Maize & Sorghum, BR-35701970 Sete Lagoas, MG, Brazil. [Mao, Chuanzao] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China. RP Kochian, LV (reprint author), Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM Leon.kochian@ars.usda.gov RI Maron, Lyza/B-5644-2008; OI Maron, Lyza/0000-0001-6475-2357; Kochian, Leon/0000-0003-3416-089X; Pineros, Miguel/0000-0002-7166-1848 FU NSF [0419435]; Generation Challenge Program [G3008.02]; USDA [2006-35301-16884]; McKnight Foundation Collaborative Crop Research Program; FAPEMIG-Brazil FX The authors would like to thank Eric Craft and Randy Clark for technical assistance during the development of this work. The work was supported by NSF Plant Genome Research Grant DBI number 0419435, Grant Generation Challenge Program grant number G3008.02, USDA National Research Initiative competitive grant number 2006-35301-16884, a McKnight Foundation Collaborative Crop Research Program grant, and a FAPEMIG-Brazil grant. NR 48 TC 95 Z9 105 U1 3 U2 45 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD MAR PY 2010 VL 61 IS 5 BP 728 EP 740 DI 10.1111/j.1365-313X.2009.04103.x PG 13 WC Plant Sciences SC Plant Sciences GA 559ER UT WOS:000274805600001 PM 20003133 ER EF