FN Thomson Reuters Web of Science™ VR 1.0 PT J AU KOMOLY, S HUDSON, LD WEBSTER, HD BONDY, CA AF KOMOLY, S HUDSON, LD WEBSTER, HD BONDY, CA TI INSULIN-LIKE GROWTH FACTOR-I GENE-EXPRESSION IS INDUCED IN ASTROCYTES DURING EXPERIMENTAL DEMYELINATION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CUPRIZONE; REMYELINATION; OLIGODENDROGLIA; MYELIN BASIC PROTEIN; INSULIN-LIKE GROWTH FACTOR-I RECEPTOR ID CENTRAL NERVOUS-SYSTEM; MYELIN BASIC-PROTEIN; CUPRIZONE-INDUCED DEMYELINATION; PROGENITOR CELLS; MESSENGER-RNAS; SOMATOMEDIN-C; IGF-I; REMYELINATION; PROLIFERATION; DEGENERATION AB To investigate insulin-like growth factor I (IGF-I) and IGF-I receptor gene expression during experimental demyelination and myelin regeneration, young mice were fed cuprizone [bis(cyclohexanone) oxaldihydrazone]. This copper-chelating agent produces demyelination in the corpus callosum and superior cerebellar peduncles, and when treatment is stopped, there is rapid remyelination. At intervals during cuprizone treatment and recovery, brain sections were hybridized with specific probes and immunostained with antibodies to determine the localization and relative amounts of IGF-I and IGF-I receptor mRNAs and peptides. In untreated littermates, IGF-I and IGF-I receptor mRNAs and peptides were not detected in white matter. In cuprizone-treated mice, high levels of both IGF-I mRNA and peptide were expressed by astrocytes in areas of myelin breakdown. Astrocyte IGF-I expression decreased rapidly during recovery and oligodendroglial expression of myelin-related genes increased. In severely demyelinated areas, immature oligodendroglia exhibited a transient increase in IGF-I receptor mRNA and peptide immunoreactivity during early recovery. This highly specific pattern of IGF-I induction in astrocytes during demyelination and the expression of the IGF-I receptor in regenerating oligodendrocytes during recovery suggest that IGF-I functions in the regulation of oligodendrocyte and myelin metabolism in vivo. C1 NINCDS,MOLEC & VIRAL PATHOGENESIS LAB,BETHESDA,MD 20892. NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP KOMOLY, S (reprint author), NINCDS,EXPTL NEUROPATHOL LAB,BLDG 36,ROOM 4A-29,BETHESDA,MD 20892, USA. NR 37 TC 205 Z9 207 U1 0 U2 3 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 1 PY 1992 VL 89 IS 5 BP 1894 EP 1898 DI 10.1073/pnas.89.5.1894 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HG681 UT WOS:A1992HG68100078 PM 1371885 ER PT J AU MAKOS, M NELKIN, BD LERMAN, MI LATIF, F ZBAR, B BAYLIN, SB AF MAKOS, M NELKIN, BD LERMAN, MI LATIF, F ZBAR, B BAYLIN, SB TI DISTINCT HYPERMETHYLATION PATTERNS OCCUR AT ALTERED CHROMOSOME LOCI IN HUMAN LUNG AND COLON CANCER SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CPG ISLANDS; ALLELIC LOSSES; GENETIC INSTABILITY ID CELL-LINES; SHORT ARM; DNA METHYLATION; DENOVO METHYLATION; MOLECULAR ANALYSIS; CPG ISLANDS; GENE; CARCINOMA; REGION; MARKERS AB Regional increases in DNA methylation occur in normally unmethylated cytosine-rich areas in neoplastic cells. These changes could potentially alter chromatin structure to inactivate gene transcription or generate DNA instability. We now show that, in human lung and colon cancer DNA, hypermethylation of such a region consistently occurs on chromosome 17p in an area that is frequently reduced to homozygosity in both tumor types. Over the progression stages of colon neoplasia, this methylation change increases in extent and precedes the allelic losses on 17p that are characteristic of colon carcinomas. We also show on chromosome 3p that regional hypermethylation may nonrandomly accompany chromosome changes in human neoplasia. Increased methylation is consistent in small-cell lung carcinoma DNA at two 3p loci that are constantly reduced to homozygosity in this tumor, but it is not seen in colon cancer DNA, in which these loci are infrequently structurally altered. C1 JOHNS HOPKINS MED INST,DEPT MED,BALTIMORE,MD 21231. NCI,FREDERICK CANC RES FACIL,FREDERICK,MD 21701. RP MAKOS, M (reprint author), JOHNS HOPKINS MED INST,CTR ONCOL,BALTIMORE,MD 21231, USA. FU NCI NIH HHS [CA43318-05, CA54396-01]; NIGMS NIH HHS [GM07814-09] NR 44 TC 168 Z9 171 U1 0 U2 4 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 1 PY 1992 VL 89 IS 5 BP 1929 EP 1933 DI 10.1073/pnas.89.5.1929 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HG681 UT WOS:A1992HG68100085 PM 1347428 ER PT J AU SAJI, M MORIARTY, J BAN, T KOHN, LD SINGER, DS AF SAJI, M MORIARTY, J BAN, T KOHN, LD SINGER, DS TI HORMONAL-REGULATION OF MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I GENES IN RAT-THYROID FRTL-5 CELLS - THYROID-STIMULATING HORMONE INDUCES A CAMP-MEDIATED DECREASE IN CLASS-I EXPRESSION SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE TRANSCRIPTION ID GRAVES-DISEASE; MESSENGER-RNA; THYROTROPIN; INTERFERON; ANTIGEN; MOUSE; THYROGLOBULIN; ACID AB Thyrocytes normally express major histocompatibility complex (MHC) class I, but not class II, cell surface antigens. A rat thyrocyte cell line, FRTL-5, also expresses MHC class I antigens, in addition to a variety of thyroid-specific genes. Treatment of FRTL-5 thyrocytes with physiological concentrations of thyroid-stimulating hormone (TSH) has been shown to induce increased expressed of thyroglobulin and thyroid peroxidase but to simultaneously decrease expression of the TSH receptor. The reduction in TSH receptor expression by TSH is cAMP mediated. In the present study, it is demonstrated that, in thyrocytes treated with TSH, MHC class I expression decreases concomitant with the decrease in TSH receptor expression. This decreased expression is evidenced by reduced cell surface levels of MHC class I antigens, by reduced steady-state RNA levels, and by reduced transcription of the class I genes. TSH-mediated reduction of MHC class I gene transcription in FRTL-5 cells was mapped to a region within 135 base pairs of the promoter. C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. RP SAJI, M (reprint author), NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892, USA. RI Saji, Motoyasu/E-4007-2011 NR 30 TC 52 Z9 52 U1 0 U2 0 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 1 PY 1992 VL 89 IS 5 BP 1944 EP 1948 DI 10.1073/pnas.89.5.1944 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HG681 UT WOS:A1992HG68100088 PM 1311856 ER PT J AU PEQUEGNAT, W GARRICK, NA STOVER, E AF PEQUEGNAT, W GARRICK, NA STOVER, E TI NEUROSCIENCE FINDINGS IN AIDS - A REVIEW OF RESEARCH SPONSORED BY THE NATIONAL INSTITUTE OF MENTAL-HEALTH SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Review DE ALPHA-MELANOCYTE STIMULATING HORMONE; GLUCOCORTICOID; HIV-1-ASSOCIATED PEPTIDES; HUMAN IMMUNODEFICIENCY VIRUS; HYPOTHALAMIC-PITUITARY-ADRENAL AXIS; INTERLEUKIN-1; MACROPHAGES; NOREPINEPHRINE; RHESUS MACAQUE MONKEYS; SIMIAN IMMUNODEFICIENCY VIRUS; STRESS-INDUCED IMMUNE ALTERATIONS; SYMPATHETIC NORADRENERGIC NERVOUS SYSTEM; LYMPHOCYTES-T; QUINOLINIC ACID ID CORTICOSTEROID RECEPTORS; NERVOUS-SYSTEM; INTERLEUKIN-1; LYMPHOCYTES; DECREASES; RESPONSES; TISSUES; BRAIN AB 1. The human immunodeficiency virus (HIV-1) infects cells in both the immune system and the brain, but these effects are not independent. 2. Research funded by the National Institute of Mental Health (NIMH) has been directed at identifying some of the mechanisms by which HIV-1 infects the brain, produces pathology, causes behavioral changes, and alters immune responses. 3. HIV-1-associated peptides have been shown to produce immunological changes without active virus present and there is also evidence that neurological damage may result not from direct viral action, by via excitotoxin production. 4. Rhesus macaque monkeys infected with simian immunodeficiency virus (SIV) are proving to be a useful model of the neurological and behavioral changes identified in human AIDS patients; behavioral changes observed in monkeys are similar to those seen in humans infected with HIV-1. 5. Studies examining the relationship between the brain and immune system are identifying the role that the macrophage cytokine interleukin-1 may play in suppressing T-lymphocyte activity by two pathways, both mediated by corticotropin releasing factor (CRF). 6. One pathway involves the pituitary-adrenal axis and release of glucocorticoids while the other involves direct interaction with the sympathetic noradrenergic nervous system, which has been shown to innervate T-lymphocytes in the spleen and thymus. 7. These observations are relevant because macrophages infected with HIV-1 infiltrate the brain and may release substances that damage the brain. 8. Stress may affect these-pathways via the CRF-mediated release of glucocorticoids; a model of stress has also demonstrated that stress can suppress the cellular immune response. RP PEQUEGNAT, W (reprint author), NIMH,OFF AIDS PROGRAMS,PARKLAWN BLDG,ROOM 17C-06,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. FU PHS HHS [352682] NR 53 TC 8 Z9 8 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PD MAR PY 1992 VL 16 IS 2 BP 145 EP 170 DI 10.1016/0278-5846(92)90067-O PG 26 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA HG512 UT WOS:A1992HG51200002 PM 1579634 ER PT J AU GIAMBRA, LM GRODSKY, A CAMP, CJ AF GIAMBRA, LM GRODSKY, A CAMP, CJ TI CURIOSITY AND STIMULATION SEEKING ACROSS THE ADULT LIFE-SPAN - CROSS-SECTIONAL AND 6-YEAR TO 8-YEAR LONGITUDINAL FINDINGS SO PSYCHOLOGY AND AGING LA English DT Article ID PERSONALITY-INVENTORY; SENSATION SEEKING; SEX-DIFFERENCES; OLDER ADULTS; AGE; YOUNG AB Giambra (1977-1978, 1979-1980) found that 2 scales of the Imaginal Processes Inventory measuring curiosity (i.e., information seeking) did not change across the adult life span, but 2 measuring stimulation seeking (i.e., boredom) for external stimulation need significantly decreased with age. In this study, these outcomes were replicated (1,356 men and 1,080 women 17 to 92 years old). In addition, a 6- to 8-year longitudinal repeat was obtained on 222 men and 124 women. Significant longitudinal declines were obtained for the stimulation-seeking measures. Furthermore, women showed an increase in impersonal-mechanical curiosity and a decline in interpersonal curiosity, though the amount of change was modest. Men were unchanged on both curiosity measures. Gender differences in longitudinal changes apparently reflected effects of socialization as well as tendencies toward displaying increased androgyny with advancing age. C1 UNIV NEW ORLEANS,NEW ORLEANS,LA 70148. RP GIAMBRA, LM (reprint author), NIA,GERONTOL RECH INST,PERSONAL & COGNIT LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 34 TC 40 Z9 42 U1 3 U2 7 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0882-7974 J9 PSYCHOL AGING JI Psychol. Aging PD MAR PY 1992 VL 7 IS 1 BP 150 EP 157 DI 10.1037/0882-7974.7.1.150 PG 8 WC Gerontology; Psychology, Developmental SC Geriatrics & Gerontology; Psychology GA HH171 UT WOS:A1992HH17100016 PM 1558700 ER PT J AU INSEL, TR AF INSEL, TR TI OXYTOCIN - A NEUROPEPTIDE FOR AFFILIATION - EVIDENCE FROM BEHAVIORAL, RECEPTOR AUTORADIOGRAPHIC, AND COMPARATIVE-STUDIES SO PSYCHONEUROENDOCRINOLOGY LA English DT Review ID CORTICOTROPIN-RELEASING FACTOR; LATENCY MATERNAL-BEHAVIOR; VENTROMEDIAL HYPOTHALAMIC NUCLEUS; RAT-BRAIN; FEMALE RATS; PARAVENTRICULAR NUCLEUS; CEREBROSPINAL-FLUID; NEUROHYPOPHYSEAL HORMONES; PENILE ERECTION; PLASMA OXYTOCIN AB Oxytocin (OT) is a nine amino acid peptide synthesized in hypothalamic cells which project either to the neurohypophysis or to sites within the central nervous system. Although neurohypophyseal OT release has long been associated with uterine contraction and milk ejection, the function of intracerebral OT remains unclear. On the basis of behavioral, cellular, and comparative studies, this review suggests that brain OT influences the formation of social bonds. The first-part of this review examines evidence linking central OT to several forms of affiliation. Central administration of OT induces maternal and reproductive behaviors in rats primed with gonadal steroids. OT antagonists and hypothalamic lesions block the initiation of maternal and reproductive behaviors but have no effects on these behaviors once established. Our new studies in rat pups demonstrate that central OT selectively decreases the separation response, an effect which mimics social contact. These studies of parental, reproductive, and attachment behaviors suggest that exogenous OT has "prosocial" effects and that endogenous OT may be essential for initiating social interaction. In a second series of experiments, we investigated the cellular mechanisms for OT's effects on social behavior by means of autoradiographic receptor binding. In the rat forebrain, OT receptors are expressed in several limbic regions believed to be involved in the integration of sensory processing. The regulation of these receptors is surprisingly resistant to either ablation of OT cells or repeated central administration of OT. However, receptors in two regions, the bed nucleus of the stria terminalis (BNST) and the ventromedial nucleus of the hypothalamus (VMN), appear selectively induced by exogenous or endogenous increases in gonadal steroids. At parturition, binding to OT receptors increases 84% in the BNST, and at estrus, binding increases 35% in the VMN. These results demonstrate that physiologic changes in gonadal steroids can alter receptor expression in anatomically discrete target fields and thereby direct responsiveness to endogenous neuropeptide release. A model for OT's effects on social behavior is proposed, which relies on the heterologous regulation of the brain OT receptor. A third series of experiments tested the hypothesis that brain OT influences affiliation by comparing prairie and montane voles, two closely related species with dichotomous systems of social organization. Although no differences appear in the presynaptic expression of the neuropeptide, OT receptors are distributed in complementary patterns in the two species. In the highly affiliative prairie vole, receptors are most evident in the BNST and one of its primary afferents, the lateral amygdala, highlighting a circuit previously implicated in maternal behavior. In the asocial montane vole, receptors are absent in these regions. At parturition, when the female montane vole first manifests maternal.behavior, the expression of OT receptors changes in the direction of the pattern observed in the highly parental prairie vole. The specificity of these findings is supported by (1) studies in other species with similar behavioral differences and (2) the failure to find concurrent species differences in the distribution of receptors for several other neurotransmitters. These various behavioral, receptor autoradiographic, and comparative studies all provide evidence that brain OT systems, via alterations in receptor number and distribution, may influence the expression of social behavior. As OT is an exclusively mammalian neuropeptide, this central neuroendocrine system may have evolved relatively recently to promote selective aspects of social bond formation. C1 NIMH, CLIN SCI LAB, POOLESVILLE, MD USA. NR 143 TC 306 Z9 308 U1 6 U2 44 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4530 J9 PSYCHONEUROENDOCRINO JI Psychoneuroendocrinology PD MAR PY 1992 VL 17 IS 1 BP 3 EP 35 DI 10.1016/0306-4530(92)90073-G PG 33 WC Endocrinology & Metabolism; Neurosciences; Psychiatry SC Endocrinology & Metabolism; Neurosciences & Neurology; Psychiatry GA HT162 UT WOS:A1992HT16200001 PM 1319071 ER PT J AU GAO, B DUNCAN, WC WEHR, TA AF GAO, B DUNCAN, WC WEHR, TA TI FLUOXETINE DECREASES BRAIN TEMPERATURE AND REM-SLEEP IN SYRIAN-HAMSTERS SO PSYCHOPHARMACOLOGY LA English DT Article DE FLUOXETINE; REM SLEEP; HYPOTHALAMIC TEMPERATURE; SYRIAN HAMSTER; SEROTONIN ID SELECTIVE INHIBITOR; BODY-TEMPERATURE; SEROTONIN UPTAKE; LILLY 110140; RAT-BRAIN; 3-(PARA-TRIFLUOROMETHYLPHENOXY)-N-METHYL-3-PHENYLPROPYLAMINE; RECEPTORS; DRUGS; AMITRIPTYLINE; CLOMIPRAMINE AB The antidepressant drug, fluoxetine (FLX), a selective serotonin reuptake inhibitor, was administered to Syrian hamsters, and its acute and chronic effects on EEG sleep and hypothalamic temperature were recorded. Acute fluoxetine treatment at doses of 5, 10, 20 and 40 mg/kg decreased REM sleep and hypothalamic temperature in a dose-dependent manner. It increased NREM sleep, and, at doses of 20 and 40 mg/kg, it increased wakefulness. At 40 mg/kg, it decreased motor activity. During chronic treatment, tolerance developed to FLX's REM sleep-inhibiting effects, but tolerance did not develop to FLX's hypothalamic temperature-decreasing effects. Chronic FLX treatment produced circadian phase-dependent decreases in temperature beyond those that were observed during acute treatment. The apparent dissociation during chronic treatment between FLX's temperature-lowering effects and its REM-decreasing effects might be related to long-term changes in 5HT receptor function or FLX pharmacokinetics. C1 NIMH,CLIN PSYCHOBIOL BRANCH,BETHESDA,MD 20892. RI Sanguansri, Luz/B-6630-2011 OI Sanguansri, Luz/0000-0003-1908-7604 NR 49 TC 25 Z9 25 U1 2 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD MAR PY 1992 VL 106 IS 3 BP 321 EP 329 DI 10.1007/BF02245412 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA HC105 UT WOS:A1992HC10500006 PM 1570377 ER PT J AU WEINGARTNER, HJ HOMMER, D LISTER, RG THOMPSON, K WOLKOWITZ, O AF WEINGARTNER, HJ HOMMER, D LISTER, RG THOMPSON, K WOLKOWITZ, O TI SELECTIVE EFFECTS OF TRIAZOLAM ON MEMORY SO PSYCHOPHARMACOLOGY LA English DT Article DE BENZODIAZEPINE; LEARNING; MEMORY; IMPLICIT MEMORY; EXPLICIT MEMORY; SEMANTIC MEMORY; AMNESIA ID IMPLICIT MEMORY; INDUCED AMNESIA; DOSE-RESPONSE; DIAZEPAM; LORAZEPAM; BENZODIAZEPINES; DISSOCIATION; PERFORMANCE; SLEEP AB The effects of a benzodiazepine (triazolam 0.25 and 0.50 mg) on different aspects of cognitive function were assessed. Triazolam impaired free recall and recognition of information presented after drug administration. In contrast to these impairments in explicit memory, a memory function that did not require conscious awareness was not altered by triazolam. Similarly, triazolam did not affect subjects' abilities to access semantic memory. C1 NIAAA,CLIN STUDIES LAB,ROCKVILLE,MD 20852. NIMH,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,DEPT PSYCHIAT,SAN FRANCISCO,CA 94143. UNIV WASHINGTON,DEPT PSYCHIAT,SEATTLE,WA 98195. RP WEINGARTNER, HJ (reprint author), NIA,GERONTOL RES CTR,COGNIT SECT,BALTIMORE,MD 21224, USA. NR 25 TC 66 Z9 66 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD MAR PY 1992 VL 106 IS 3 BP 341 EP 345 DI 10.1007/BF02245415 PG 5 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA HC105 UT WOS:A1992HC10500009 PM 1570380 ER PT J AU PUTNAM, LE JOHNSON, R ROTH, WT AF PUTNAM, LE JOHNSON, R ROTH, WT TI GUIDELINES FOR REDUCING THE RISK OF DISEASE TRANSMISSION IN THE PSYCHOPHYSIOLOGY LABORATORY SO PSYCHOPHYSIOLOGY LA English DT Article; Proceedings Paper CT 31ST ANNUAL MEETING OF THE SOC FOR PSYCHOPHYSIOLOGICAL RESEARCH CY OCT, 1991 CL CHICAGO, IL SP SOC PSYCHOPHYSIOL RES DE LABORATORY SAFETY; DISEASE TRANSMISSION; PSYCHOPHYSIOLOGY; ELECTRODES; DISINFECTION; AIDS; HEPATITIS ID III/LYMPHADENOPATHY-ASSOCIATED VIRUS; LYMPHADENOPATHY-ASSOCIATED VIRUS; INACTIVATION; DISINFECTANTS; PRECAUTIONS; INFECTION; PERSONNEL AB The acquired immunodeficiency syndrome (AIDS) pandemic has highlighted the need for safeguards against the inadvertent transmission of infectious disease in the psychophysiology laboratory. These Guidelines identify factors contributing to the risk of bloodborne disease transmission to subjects or technicians, and recommend procedures to minimize such risk, given current knowledge and techniques. The lowest risk is associated with the application of devices, such as surface electrodes, to non-abraded, intact skin. Such devices should be clean, but do not require disinfection. The potential risk of infection is higher when surface electrodes are applied to non-intact skin. Abrasion, or other breaks in the skin, can allow seepage of blood products carrying such pathogens as hepatitis B virus and the human immunodeficiency virus that causes AIDS. Thus electrodes require high-level disinfection before reuse on non-intact skin. In addition, technicians should wear gloves during skin preparation and should abrade the skin no more than necessary, using only sterile, preferably non-sharp materials. The highest risk is that associated with items that enter sterile tissue, such as subdermal electrodes and the needles and lancets sometimes used in skin preparation. Such items must be sterile at the time of use and must be handled with extreme caution. C1 NIH,BETHESDA,MD 20892. COLUMBIA UNIV,NEW YORK,NY 10027. STANFORD UNIV,MED CTR,STANFORD,CA 94305. NR 39 TC 31 Z9 31 U1 0 U2 1 PU SOC PSYCHOPHYSIOL RES PI WASHINGTON PA 1010 VERMONT AVE NW SUITE 1100, WASHINGTON, DC 20005 SN 0048-5772 J9 PSYCHOPHYSIOLOGY JI Psychophysiology PD MAR PY 1992 VL 29 IS 2 BP 127 EP 141 PG 15 WC Psychology, Biological; Neurosciences; Physiology; Psychology; Psychology, Experimental SC Psychology; Neurosciences & Neurology; Physiology GA HX011 UT WOS:A1992HX01100001 PM 1635955 ER PT J AU HAYTHORNTHWAITE, JA PRATLEY, RE ANDERSON, DE AF HAYTHORNTHWAITE, JA PRATLEY, RE ANDERSON, DE TI BEHAVIORAL STRESS POTENTIATES THE BLOOD-PRESSURE EFFECTS OF A HIGH SODIUM-INTAKE SO PSYCHOSOMATIC MEDICINE LA English DT Article ID DIETARY-SODIUM; YOUNG BLACKS; HYPERTENSION; SENSITIVITY; SALT; AGE; NOREPINEPHRINE; WHITES; HUMANS; RACE AB The present study examined the blood pressure effects of a high sodium intake administered during two levels of behavioral stress. In this double-blind study, 32 medical students were randomly assigned to receive either sodium chloride (high sodium) or placebo tablets (usual sodium). Resting blood pressure and body weight were recorded across a 14-day period preceding examinations (high stress) and during the summer when academic demands were reduced (low stress). The high sodium intake during the high stress period was associated with greater elevations in resting systolic blood pressure and mean arterial pressure than either the usual sodium intake during the high stess period or the high sodium intake during a low stress period. These findings suggest that behaviorally induced neuroendocrine responses can potentiate a blood pressure response to a high sodium intake. C1 UNIV MARYLAND, BALTIMORE, MD 21201 USA. RP HAYTHORNTHWAITE, JA (reprint author), NIA, GERONTOL RES CTR, BEHAV SCI LAB, 4940 EASTERN AVE, BALTIMORE, MD 21224 USA. NR 41 TC 18 Z9 18 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD MAR-APR PY 1992 VL 54 IS 2 BP 231 EP 239 PG 9 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA HL760 UT WOS:A1992HL76000008 PM 1565758 ER PT J AU LANGE, WR FRANKENFIELD, DL CARICO, J PFEIFFER, MB SNYDER, FR VANDERDECKER, J AF LANGE, WR FRANKENFIELD, DL CARICO, J PFEIFFER, MB SNYDER, FR VANDERDECKER, J TI DEATHS AMONG MEMBERS OF THE PUBLIC-HEALTH SERVICE COMMISSIONED CORPS, 1965-89 SO PUBLIC HEALTH REPORTS LA English DT Article ID UNITED-STATES; MILITARY PERSONNEL; PHYSICIANS; MORTALITY; SUICIDE AB The U.S. Public Health Service Commissioned Corps performs health promotion and disease prevention activities and provides clinical care. The authors examined the epidemiology of deaths among active duty personnel and the hypothesis that, based on the mission, mortality would be less than in the general population, and that deaths would reflect nonpreventable causes. A retrospective record review for the period 1965-89 showed 118 active duty deaths, 26 percent of the number anticipated in a general population group adjusted for age, sex, and race or ethnicity. The five major causes of death were coronary heart disease, suicide, motor vehicle crash, malignant neoplasm, and drowning. Beginning with the mid-1980s, infectious disease became a principal cause of death, the only cause for which the rate trended upward. Among professionals, death rates were highest among sanitarians and veterinarians, and lowest among pharmacists. The only causes for which deaths exceeded the expected number involved suicides and possibly deaths related to acquired immunodeficiency syndrome. Active duty status in the Commissioned Corps was associated with a death rate less than that of comparable groups in the general population. Many of the premature deaths were attributable to preventable causes. C1 NIDA,ADDICT RES CTR,OFF ASSISTANT SECRETARY HLTH,LEXINGTON,KY 40583. NIDA,ADDICT RES CTR,DIV COMMISSIONED PERSONNEL,LEXINGTON,KY 40583. NR 21 TC 5 Z9 5 U1 0 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0033-3549 J9 PUBLIC HEALTH REP JI Public Health Rep. PD MAR-APR PY 1992 VL 107 IS 2 BP 160 EP 166 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA HN004 UT WOS:A1992HN00400007 PM 1561297 ER PT J AU DOUEK, P BONNER, RF AF DOUEK, P BONNER, RF TI FLUORESCENCE-GUIDED PULSED DYE LASER-ASSISTED ANGIOPLASTY - REPLY SO RADIOLOGY LA English DT Letter RP DOUEK, P (reprint author), NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892, USA. RI Bonner, Robert/C-6783-2015 NR 5 TC 0 Z9 0 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD MAR PY 1992 VL 182 IS 3 BP 897 EP 897 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA HE860 UT WOS:A1992HE86000057 ER PT J AU ROTH, CA LENFANT, C AF ROTH, CA LENFANT, C TI FUNDING BIOMEDICAL-RESEARCH - A COMMON GROUND FOR CONCERN SO RESPIRATION PHYSIOLOGY LA English DT Article DE FUNDING; BIOMEDICAL RESEARCH AB Despite record levels of support, concerns over the ability of individual investigators to obtain funding for their research have been growing within the biomedical research community. The concerns have been focused almost exclusively upon the outcomes of funding decisions, and have ignored the pressures that produce them. Yet, it is those pressures that constitute our 'common ground for concern'. Resources committed in response to the pressures from special interest groups are unavailable for general competition based upon scientific merit. We in the biomedical research community must recognize that such self-interested efforts to dedicate resources undermine the integrity of the existing processes for making funding decisions. As a community, we should instead attempt to ensure that adequate resources are available to support the best quality research. RP ROTH, CA (reprint author), NHLBI,BLDG 31,ROOM 5A52,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0034-5687 J9 RESP PHYSIOL JI Respir. Physiol. PD MAR PY 1992 VL 87 IS 3 BP 269 EP 274 DI 10.1016/0034-5687(92)90011-K PG 6 WC Physiology; Respiratory System SC Physiology; Respiratory System GA HP865 UT WOS:A1992HP86500002 PM 1604052 ER PT J AU HOULT, DI PRESTON, CM AF HOULT, DI PRESTON, CM TI INEXPENSIVE PLASMA DISCHARGE SOURCE FOR MOLECULAR-EMISSION SPECTROSCOPY WITH APPLICATION TO N-15 ANALYSIS SO REVIEW OF SCIENTIFIC INSTRUMENTS LA English DT Article ID SPECTROMETRY AB The assaying of nitrogen isotope abundance using emission spectroscopy commonly requires a plasma discharge in a sample of N2 gas at low pressure (approximately 0.5 kPa) in a sealed glass tube. Expensive high-power (> 20 W) radio-frequency or microwave amplifiers are often used for this purpose. However, with correct design, a simple and inexpensive, single transistor, 100 MHz, 1 W oscillator can be used to produce a bright plasma discharge in a low-pressure sample of nitrogen in a 6 mm diameter by 10 cm tube. The device utilizes optimal impedance matching to minimize the power requirements. The principles and limitations of the matching are outlined, and a simple feedback circuit is also described which, with the aid of a phototransistor, enables the emission intensity to be controlled. Ignition of discharge by friction or with the help of a simple piezoelectric gas lighter is also described, and construction details are given. C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 8 TC 4 Z9 4 U1 1 U2 3 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0034-6748 J9 REV SCI INSTRUM JI Rev. Sci. Instrum. PD MAR PY 1992 VL 63 IS 3 BP 1927 EP 1931 DI 10.1063/1.1143306 PG 5 WC Instruments & Instrumentation; Physics, Applied SC Instruments & Instrumentation; Physics GA HG449 UT WOS:A1992HG44900012 ER PT J AU PIEGORSCH, WW CARR, GJ PORTIER, CJ HOEL, DG AF PIEGORSCH, WW CARR, GJ PORTIER, CJ HOEL, DG TI CONCORDANCE OF CARCINOGENIC RESPONSE BETWEEN RODENT SPECIES - POTENCY DEPENDENCE AND POTENTIAL UNDERESTIMATION SO RISK ANALYSIS LA English DT Article DE CARCINOGENIC POTENCY; STRATIFICATION; TOXICITY; AVERAGING PROCESS; CORRELATION; CONCORDANCE UNDERESTIMATION ID GENETIC TOXICITY ASSAYS; ANIMAL BIOASSAYS; CHEMICALS; RATS; DATABASE; MICE; PREDICTIVITY AB The use of average qualitative concordance between two bioassay endpoints is considered, with emphasis directed at agreement between rats and mice from results of long-term carcinogenicity studies. It is noted that concordance varies as a function of the underlying potency or toxicity of the chemicals over which the averaging is performed. Thus, the averaging process dilutes large observed concordances from potent chemicals, and possibly inflates lower observed concordances from weakly active chemicals. Stratification over some measure of potency is suggested as a method for taking these effects into account. Statistical simulations of concordance analyses limited to low-potency ranges are employed to examine the concordance measure in greater detail. It is seen that at low potencies, observed concordance is consistently underestimated, reaching maximum levels of only about 80%. C1 MERRELL DOW PHARMACEUT INC,DEPT BIOSTAT,CINCINNATI,OH 45215. RP PIEGORSCH, WW (reprint author), NIEHS,DIV BIOMETRY & RISK ASSESSMENT,RES TRIANGLE PK,NC 27709, USA. RI Portier, Christopher/A-3160-2010; OI Portier, Christopher/0000-0002-0954-0279; Piegorsch, Walter/0000-0003-2725-5604 NR 28 TC 18 Z9 18 U1 1 U2 2 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD MAR PY 1992 VL 12 IS 1 BP 115 EP 121 DI 10.1111/j.1539-6924.1992.tb01314.x PG 7 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA HJ872 UT WOS:A1992HJ87200013 PM 1574611 ER PT J AU HENSON, DE RIES, L SHAMBAUGH, EM AF HENSON, DE RIES, L SHAMBAUGH, EM TI SURVIVAL RESULTS DEPEND ON THE STAGING SYSTEM SO SEMINARS IN SURGICAL ONCOLOGY LA English DT Article DE TNM; AJCC; BREAST CANCER; COLON CANCER; LOCALIZED, REGIONAL, DISTANT STAGING AB The results of expressing patient outcome are compared using two staging systems: localized, regional, and distant (LRD) and the TNM of the American Joint Committee on Cancer (AJCC). Expressing patient outcome depends on the staging system used. There is overlap between the stage definitions of the LRD and the TNM. A single stage in the LRD may include more than one stage grouping of the TNM and vice versa. For most sites, "localized" provides lower survival rates than stage I of the TNM. The TNM provides more precise information about prognosis because its definitions reflect the latest survival information and diagnostic technology. Time trends can be measured only with the LRD because of its stability over the years. The precision of the TNM has been achieved at the expense of time trend analysis. The LRD is usually not an acceptable end point for the assessment of early cancer detection. RP HENSON, DE (reprint author), NCI,DIV CANC PREVENT & CONTROL,EPN,SUITE 305,BETHESDA,MD 20892, USA. NR 0 TC 21 Z9 21 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 8756-0437 J9 SEMIN SURG ONCOL JI Semin. Surg. Oncol. PD MAR-APR PY 1992 VL 8 IS 2 BP 57 EP 61 DI 10.1002/ssu.2980080203 PG 5 WC Oncology; Surgery SC Oncology; Surgery GA HQ386 UT WOS:A1992HQ38600002 PM 1615264 ER PT J AU SMART, CR CHU, KC AF SMART, CR CHU, KC TI STAGING PATTERNS AND EARLY CANCER-DETECTION SO SEMINARS IN SURGICAL ONCOLOGY LA English DT Article DE STAGE SHIFTS; SCREENING; EXTENT OF DISEASE AB There is a great deal of indirect, nonexperimental evidence that a pattern of earlier-stage disease at diagnosis has a better outcome. Increased early detection activities can change, these stage patterns while various biases and the question of generalizability need to be kept in mind in their interpretation. The indirect evidences of possible benefit from early detection activities includes an increase in the number of cases detected, a pattern of more early- and less advanced-stage cases, an increase in the overall site-specific survival rate, and a decrease in the case fatality rate. Unless these intermediate markers are favorable, it is unlikely that early detection will reduce mortality. In addition, one should also differentiate a reduced incidence or a change in treatment as a cause for reduced mortality. C1 NCI,DIV CANC PREVENT & CONTROL,EARLY DETECT BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 8756-0437 J9 SEMIN SURG ONCOL JI Semin. Surg. Oncol. PD MAR-APR PY 1992 VL 8 IS 2 BP 62 EP 72 DI 10.1002/ssu.2980080204 PG 11 WC Oncology; Surgery SC Oncology; Surgery GA HQ386 UT WOS:A1992HQ38600003 PM 1615265 ER PT J AU WASSERHEIT, JN AF WASSERHEIT, JN TI EPIDEMIOLOGIC SYNERGY - INTERRELATIONSHIPS BETWEEN HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION AND OTHER SEXUALLY-TRANSMITTED DISEASES - (REPRINTED FROM AIDS AND WOMENS REPRODUCTIVE HEALTH, CH 5, 1992) SO SEXUALLY TRANSMITTED DISEASES LA English DT Reprint ID HERPES-SIMPLEX VIRUS; HUMAN PAPILLOMAVIRUS INFECTION; ACYCLOVIR-RESISTANT HERPES; CERVICAL INTRAEPITHELIAL NEOPLASIA; PNEUMOCYSTIS-CARINII PNEUMONIA; PELVIC INFLAMMATORY DISEASE; ACQUIRED IMMUNE-DEFICIENCY; NATIONAL CASE-CONTROL; FEMALE GENITAL-TRACT; HIV-INFECTION AB Understanding the role of other sexually transmitted diseases (STDs) in the transmission of human immunodeficiency virus (HIV), the role of STDs in progression of HIV disease, and the role of HIV infection in alterations of natural history, diagnosis, or response to therapy of STDs is critical to the development of optimal strategies for HIV control. One hundred sixty-three studies on the interrelationships between HIV infection and other STDs were examined. Of 75 studies on the role of STDs in HIV transmission, the 15 analyses of examination or laboratory evidence of STDs adjusted for sexual behavior showed that both ulcerative and nonulcerative STDs increase the risk of HIV transmission approximately 3- to 5-fold. Due to limited data, the role of STDs in progression of disease remains unclear. Preliminary data from 83 reports on the impact of HIV infection on STDs suggest that, at a community level, HIV infection may increase the prevalence of some STDs (e.g., genital ulcers). If coinfection with HIV prolongs or augments the infectiousness of individuals with STDs, and if the same STDs facilitate transmission of HIV, these infections may greatly amplify one another. This "epidemiological synergy" may be responsible for the explosive growth of the HIV pandemic in some populations. Effective STD control programs will be essential to HIV prevention in these communities. RP WASSERHEIT, JN (reprint author), NIAID,SEXUALLY TRANSMITTED DIS BRANCH,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 205 TC 1007 Z9 1030 U1 5 U2 24 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0148-5717 J9 SEX TRANSM DIS JI Sex. Transm. Dis. PD MAR-APR PY 1992 VL 19 IS 2 BP 61 EP 77 PG 17 WC Infectious Diseases SC Infectious Diseases GA HK774 UT WOS:A1992HK77400001 PM 1595015 ER PT J AU MILLER, H KUBICEK, M AF MILLER, H KUBICEK, M TI GRANTS AWARDED TO ESTABLISH 5 SEXUALLY-TRANSMITTED DISEASE COOPERATIVE RESEARCH CENTERS SO SEXUALLY TRANSMITTED DISEASES LA English DT Article RP MILLER, H (reprint author), NIAID,SEXUALLY TRANSMITTED DIS BRANCH,SOLAR BLDG,ROOM 3AZ6,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0148-5717 J9 SEX TRANSM DIS JI Sex. Transm. Dis. PD MAR-APR PY 1992 VL 19 IS 2 BP 121 EP 122 PG 2 WC Infectious Diseases SC Infectious Diseases GA HK774 UT WOS:A1992HK77400011 PM 1595014 ER PT J AU FLEISCHMANN, RD JENG, C GOTTESMAN, MM AF FLEISCHMANN, RD JENG, C GOTTESMAN, MM TI ABLATION OF STIMULATION OF A CAMP-RESPONSIVE PROMOTER IN CHO CELL-LINES DEFECTIVE IN THEIR CAMP-DEPENDENT PROTEIN-KINASE SYSTEM SO SOMATIC CELL AND MOLECULAR GENETICS LA English DT Article ID TYROSINE AMINOTRANSFERASE GENE; RAT SOMATOSTATIN GENE; HAMSTER OVARY CELLS; ALPHA-SUBUNIT GENE; CYCLIC-AMP; REGULATORY SUBUNIT; DNA-BINDING; TRANSCRIPTION FACTOR; NORMAL FIBROBLASTS; CATALYTIC SUBUNIT AB We have studied the requirement for an intact cAMP-dependent protein kinase (PKA) system to regulate cAMP-mediated gene transcription in Chinese hamster ovary (CHO) cells. Wild-type CHO cells and mutant CHO cell lines selected for their resistance to the growth inhibitory effect of 8-Br-cAMP and defective in their PKA system were transiently transfected with reporter plasmids containing 2.5 and 3.0 kb of the 5'-flanking sequence of the rat tyrosine aminotransferase (TAT) gene promoter. This segment of DNA contains no CRE-like sequences, yet wild-type transfectants exhibited a specific increase in TAT promoter activity following growth in medium containing 8-Br-cAMP. In CHO cell lines defective in their PKA, the transfected TAT promoter failed to respond to cAMP treatment. We conclude that an intact PKA system is necessary for the cAMP-mediated increase in TAT promoter activity in CHO cells and that there is no requirement for a CRE to see this effect. RP FLEISCHMANN, RD (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 50 TC 4 Z9 4 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0740-7750 J9 SOMAT CELL MOLEC GEN JI Somat.Cell Mol.Genet. PD MAR PY 1992 VL 18 IS 2 BP 103 EP 111 DI 10.1007/BF01233157 PG 9 WC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity GA HZ107 UT WOS:A1992HZ10700001 PM 1349445 ER PT J AU WHITEHEAD, RE SUGAWARA, O MARONPOT, RR GLADEN, BC BARRETT, JC AF WHITEHEAD, RE SUGAWARA, O MARONPOT, RR GLADEN, BC BARRETT, JC TI DETECTION OF MULTIPLE TUMOR SUPPRESSOR GENES FOR SYRIAN-HAMSTER FIBROSARCOMAS BY SOMATIC-CELL HYBRIDIZATION SO SOMATIC CELL AND MOLECULAR GENETICS LA English DT Article ID HUMAN RETINOBLASTOMA GENE; NEOPLASTIC TRANSFORMATION; COLORECTAL CARCINOMAS; CHROMOSOME-18Q GENE; RAT CHROMOSOME-5; P53; IDENTIFICATION; MALIGNANCY; MUTATIONS; SEQUENCE AB Identification of tumor suppressor gene loci in rodent cell culture systems has relied upon the use of somatic cell hybridization studies. Although normal rodent fibroblasts are capable of suppressing the tumorigenicity of a variety of tumor cells, the lack of complementation in tumor cell x tumor cell hybrids has left the possibility that a single tumor suppressor gene may be responsible for tumor suppression in a particular rodent cell culture system. Using this same approach, we found no evidence for complementation resulting in suppression of the transformed phenotype when three viral oncogene-transformed Syrian hamster embryo (SHE) cell lines and one spontaneously transformed baby hamster kidney (BHK) cell line were fused to benzo[a]pyrene-transformed SHE cells (BP6T-M3). However, v-src oncogene-transformed cell line (srcT) x BP6T-M3 hybrids did demonstrate limited suppression of the transformed phenotype, suggesting at least two complementing tumor suppressor genes in this system. We were able to confirm and extend this finding using another experimental approach with preneoplastic hamster cell lines that are immortal in culture but nontumorigenic in nude mice. We propose that fusion of these preneoplastic cells to various tumor cells may reveal tumor suppressor genes not evident in the tumor cell X tumor cell complementation studies. Subclones of two nontumorigenic, immortal hamster cell lines, 10W and DES4, displayed differing abilities to suppress BP6T-M3 cells in somatic cell hybrids, as quantitated by the ability of the hybrid cells to form colonies in soft agar. With a panel of preneoplastic hamster cell x BP6T-M3 hybrids, a distinct pattern of suppression or expression of the transformed phenotype was observed. Marked differences in this pattern were seen when the same 10W and DES4 subclones were fused to other hamster fibrosarcoma cell lines, indicating different tumor suppressing activities of multiple tumor suppressor genes. Analysis of this data suggests that as few as three or as many as six different tumor suppressor genes may be active in the Syrian hamster embryo cell culture system. Thus, this system may provide a useful model for identifying and studying the effects and regulation of a number of different tumor suppressor genes for fibrosarcomas. C1 UNIV N CAROLINA,CURRICULUM TOXICOL,CHAPEL HILL,NC 27599. RP WHITEHEAD, RE (reprint author), NIEHS,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 44 TC 8 Z9 8 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0740-7750 J9 SOMAT CELL MOLEC GEN JI Somat.Cell Mol.Genet. PD MAR PY 1992 VL 18 IS 2 BP 131 EP 142 DI 10.1007/BF01233160 PG 12 WC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity GA HZ107 UT WOS:A1992HZ10700004 PM 1574739 ER PT J AU GORDON, SL AF GORDON, SL TI FEDERAL-POLICIES REGARDING SCIENTIFIC INTEGRITY IN BIOMEDICAL-RESEARCH SO SRA-JOURNAL OF THE SOCIETY OF RESEARCH ADMINISTRATORS LA English DT Editorial Material RP GORDON, SL (reprint author), NIAMSD,MUSCULOSKELETAL DIS BRANCH,BETHESDA,MD 20892, USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU SOC RESEARCH ADMINISTRATORS PI WASHINGTON PA 1200 19TH ST, NW, STE 300, WASHINGTON, DC 20036 SN 1062-8142 J9 SRA-J SOC RES ADMIN JI SRA-J. Soc. Res. Admin. PD SPR PY 1992 VL 23 IS 4 BP 43 EP 46 PG 4 WC Business; Management SC Business & Economics GA HW854 UT WOS:A1992HW85400006 ER PT J AU BAKER, SG ROSENBERGER, WF DERSIMONIAN, R AF BAKER, SG ROSENBERGER, WF DERSIMONIAN, R TI CLOSED-FORM ESTIMATES FOR MISSING COUNTS IN 2-WAY CONTINGENCY-TABLES SO STATISTICS IN MEDICINE LA English DT Article ID MAXIMUM-LIKELIHOOD ESTIMATION; NONRESPONSE; PREGNANCY; SMOKING; MODELS; VARIABLES AB One method for analyzing contingency tables with missing observations is to model the missing-data mechanism using log-linear models. Previous methods for obtaining estimates (of missing counts and parameters) have required an iterative algorithm. In many cases, however, one can obtain estimates by use of a simple algebraic formula. We illustrate the method with data on smoking and birth weight. C1 GEORGE WASHINGTON UNIV,CTR BIOSTAT,DEPT STAT COMP & INFORMAT SYST,ROCKVILLE,MD 20852. NICHHD,BIOMETRY BRANCH,BETHESDA,MD 20892. RP BAKER, SG (reprint author), NCI,DIV CANC PREVENT & CONTROL,BIOMETRY BRANCH,EPN 344,BETHESDA,MD 20892, USA. NR 26 TC 51 Z9 51 U1 0 U2 3 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD MAR PY 1992 VL 11 IS 5 BP 643 EP 657 DI 10.1002/sim.4780110509 PG 15 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA HT432 UT WOS:A1992HT43200008 PM 1594807 ER PT J AU RAY, R VICCHIO, D YERGEY, A HOLICK, MF AF RAY, R VICCHIO, D YERGEY, A HOLICK, MF TI SYNTHESIS OF 25-HYDROXY-[6,19,19'-(H-3)2]VITAMIN-D3 AND 1-ALPHA,25-DIHYDROXY-[6,19,19'-(H-3)2]VITAMIN-D3 SO STEROIDS LA English DT Article DE SYNTHESIS; 25-HYDROXYVITAMIN-D3; 1-ALPHA,25-DIHYDROXYVITAMIN-D3; DEUTERIUM LABELING; THERMOSPRAY MASS SPECTROMETRY; METABOLIC STUDIES; STEROIDS ID VITAMIN-D; MASS-SPECTROMETRY; METABOLISM; 1-ALPHA,25-DIHYDROXYVITAMIN-D3; 1,25-DIHYDROXYVITAMIN-D3; KIDNEY; RAT AB Syntheses of polydeuterated analogs of 25-hydroxyvitamin D3 and 1-alpha,25-dihydroxy vitamin D3 are described. These analogs, containing stable isotope atoms at metabolically stable positions, are potentially useful in studies involving catabolism of hydroxylated metabolites of vitamin D3. C1 BOSTON UNIV,SCH MED,VITAMIN D LAB,BOSTON,MA 02118. NIH,BETHESDA,MD 20892. FU NIAMS NIH HHS [R01-AR36963] NR 19 TC 8 Z9 8 U1 0 U2 1 PU BUTTERWORTH-HEINEMANN PI WOBURN PA 225 WILDWOOD AVE #UNITB PO BOX 4500, WOBURN, MA 01801-2084 SN 0039-128X J9 STEROIDS JI Steroids PD MAR PY 1992 VL 57 IS 3 BP 142 EP 146 DI 10.1016/0039-128X(92)90072-H PG 5 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA HG789 UT WOS:A1992HG78900007 PM 1621268 ER PT J AU JACOBS, TP KEMPSKI, O MCKINLEY, D DUTKA, AJ HALLENBECK, JM FEUERSTEIN, G AF JACOBS, TP KEMPSKI, O MCKINLEY, D DUTKA, AJ HALLENBECK, JM FEUERSTEIN, G TI BLOOD-FLOW AND VASCULAR-PERMEABILITY DURING MOTOR DYSFUNCTION IN A RABBIT MODEL OF SPINAL-CORD ISCHEMIA SO STROKE LA English DT Article DE BLOOD BRAIN BARRIER; SPINAL CORD; RABBITS ID EXPERIMENTAL CEREBRAL INFARCTION; BRAIN EDEMA; STROKE; HISTOPATHOLOGY; HYPEREMIA; CHILDREN AB Background and Purpose: Delayed deterioration of neurological function after central nervous system ischemia is a well-documented clinical problem. The purpose of our study was to elucidate the role of spinal cord blood flow and spinal cord-blood barrier integrity in the evolution of delayed neurological deterioration after transient spinal cord ischemia in rabbits. Methods: Anesthetized rabbits were subjected to lumbar spinal cord ischemia (25 minutes) and variable periods of reperfusion (30 minutes to 48 hours after ischemia). Regional spinal cord blood flow was monitored by carbon-14-labeled iodoantipyrine autoradiography; vascular permeability was assessed by quantitative microhistofluorescence of Evans blue-albumin in frozen sections of spinal cord. Hindlimb motor function was assessed by standard scoring system and tissue edema by wet/dry weight method. Results: Hindlimb motor function indicated complete paralysis during ischemia and partial gradual recovery upon reperfusion (up to 8 hours), followed by progressive deterioration to severe deficits over 48 hours. Severe vascular permeability disruption was noticed early (30 minutes) after reperfusion, but almost complete recovery reestablished at 8 hours was followed by a secondary progressive increase in vascular permeability. Blood flow was reduced by 20-30% (p < 0.01) 4 hours after ischemia in the gray matter, but hyperemia (200-300%, p < 0.01) was observed 12-24 hours after ischemia. Spinal cord water content increased by 5.7% (p < 0.05) 24 hours after ischemia. Conclusions: This study demonstrates that delayed neurological and motor deterioration after spinal cord ischemia is associated with severe progressive breakdown of spinal cord-blood barrier integrity that develops late (hours) after the injury. Our data suggest that no ischemic insult in early or late reperfusion is associated with delayed motor deterioration. C1 SMITHKLINE BEECHAM PHARMACEUT, DIV PHARMACOL, POB 1539, UW2511, KING OF PRUSSIA, PA 19406 USA. UNIV MAINZ KLINIKUM, INST NEUROCHIRURG PATHOPHYSIOL, W-6500 MAINZ 1, GERMANY. SMITHKLINE BEECHAM PHARMACEUT, DIV PHARMACOL, KING OF PRUSSIA, PA USA. GEISINGER MED CTR, DEPT PEDIAT, DANVILLE, PA 17822 USA. NIH, BETHESDA, MD 20892 USA. NINCDS, BETHESDA, MD 20892 USA. NATL NAVAL MED CTR, NEUROL CLIN, BETHESDA, MD 20814 USA. NR 29 TC 64 Z9 72 U1 0 U2 2 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0039-2499 J9 STROKE JI Stroke PD MAR PY 1992 VL 23 IS 3 BP 367 EP 373 PG 7 WC Clinical Neurology; Peripheral Vascular Disease SC Neurosciences & Neurology; Cardiovascular System & Cardiology GA HH219 UT WOS:A1992HH21900010 PM 1542898 ER PT J AU RUBIN, JT LOTZE, MT AF RUBIN, JT LOTZE, MT TI ACUTE GASTRIC-MUCOSAL INJURY ASSOCIATED WITH THE SYSTEMIC ADMINISTRATION OF INTERLEUKIN-4 SO SURGERY LA English DT Article ID ACTIVATED KILLER ACTIVITY; CELL-PROLIFERATION; DUODENAL-ULCER; HUMAN-LYMPHOCYTES; BLOOD-FLOW; T-CELLS; IL-4; INDUCTION; PROSTAGLANDINS; ASPIRIN AB Methods. Seventy-three patients with advanced malignancy were treated with the recombinant lymphokine interleukin-4 either as the sole immunotherapeutic reagent or in combination with recombinant interleukin-2. Results. Twelve of 84 courses of therapy were complicated by gastroduodenal erosion or ulceration. Three of these courses were associated with significant bleeding, which required multiple red blood cell transfusions and endoscopic therapy. No treatment-related deaths occurred. Eleven of 57 courses administered with concomitant indomethacin and 11 of 62 courses administered with ranitidine resulted in gastroduodenal mucosal injury. No acute change in gastric acid output occurred after one dose of interleukin-4 in patients prospectively evaluated with an indwelling nasogastric tube. An intravenous ranitidine infusion appropriately reduced acid output in these patients. In contrast, we have treated over 650 patients with interleukin-2 and indomethacin without interleukin-4, none of whom developed signs or symptoms of gastroduodenal ulceration. Conclusions. These observations suggest that systemically administered cytokines may exert an effect on the integrity of the gastroduodenal mucosa. C1 NCI,SURG BRANCH,BETHESDA,MD 20892. NR 40 TC 17 Z9 17 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0039-6060 J9 SURGERY JI Surgery PD MAR PY 1992 VL 111 IS 3 BP 274 EP 280 PG 7 WC Surgery SC Surgery GA HG787 UT WOS:A1992HG78700006 PM 1542854 ER PT J AU JOYCE, JN LEXOW, N KIM, SJ ARTYMYSHYN, R SENZON, S LAWERENCE, D CASSANOVA, MF KLEINMAN, JE BIRD, ED WINOKUR, A AF JOYCE, JN LEXOW, N KIM, SJ ARTYMYSHYN, R SENZON, S LAWERENCE, D CASSANOVA, MF KLEINMAN, JE BIRD, ED WINOKUR, A TI DISTRIBUTION OF BETA-ADRENERGIC-RECEPTOR SUBTYPES IN HUMAN POSTMORTEM BRAIN - ALTERATIONS IN LIMBIC REGIONS OF SCHIZOPHRENICS SO SYNAPSE LA English DT Article DE CORTEX; HIPPOCAMPUS; BASAL GANGLIA; BETA1 (BETA-1) RECEPTOR; BETA2 (BETA-2) RECEPTOR ID NEUROLEPTIC-INDUCED AKATHISIA; BETA-2-ADRENERGIC RECEPTORS; SUICIDE VICTIMS; RAT-BRAIN; QUANTITATIVE AUTORADIOGRAPHY; H-3 DIHYDROALPRENOLOL; SELECTIVE INCREASES; HUMAN STRIATUM; UPTAKE SITES; BINDING AB The distribution of the beta-1 (beta-1) beta-2 (beta-2) subtypes of the beta-adrenergic receptor was examined in rat and nondiseased control human tissue. The distribution of the beta-1 and beta-2 receptors was also examined in schizophrenic cases, with additional studies in schizophrenic suicide and nonschizophrenic suicide cases. Scatchard analysis of the binding of [I-125]iodopindolol (IPIN) to cortical membranes showed a similar K(d) in human (177 pM) and rat (161 pM), but a lower maximum binding site (B(max)) in the human tissue (18.7 fmol/mg protein and 55.6 fmol/mg protein). For the autoradiographic studies [I-125]IPIN was used to visualize both subtypes (total) or was displaced with the selective beta-1-receptor antagonist ICI-89,406 to visualize beta-2 sites, or with the selective beta-2-receptor antagonist ICI-118,551 to visualize beta-1, sites. Important differences in the regional distribution of the two subtypes of the beta-adrenergic receptors were noted between rat and human. In the nucleus accumbens and ventral putamen (ventral striatum), a patchy distribution of beta-1, receptors was observed that was not evident in the rat. These patches were aligned with markers of the matrix compartment of the striatum. The schizophrenic cases showed significant increases in the labeling of the beta-1-receptor patches with [I-125]IPIN. In contrast to the frontal cortex of the nondisease controls, the parietal and temporal cortex showed a high ratio of beta-1 to beta-2 receptors and a highly laminar organization of the subtypes. [I-125]IPIN binding to beta-1 receptors was highest in the external laminae with the reverse gradient for the beta-2 subtype. The medial temporal cortex displayed an alteration in the ratio of the 2 subtypes of the beta-adrenergic receptor, with the parahippocampus and hippocampus of the human, in contrast to the rat brain, predominantly expressing the beta-2 receptor. Moreover, there were consistently higher densities of beta-2 receptors in the hippocampus of the right hemisphere than the left hemisphere of the nondisease controls. There was not a left and right hemispheric asymmetry of beta-2 receptors in the hippocampus of elderly schizophrenics or in young schizophrenics who committed suicide. The asymmetry was evident in nonschizophrenic suicides, suggesting that the lack of asymmetry in the hippocampus of schizophrenics is evident early in the disease process. Thus limbic structures show alterations in the patterning of beta-1 and beta-2 receptors in the schizophrenic cases. C1 UNIV PENN,SCH MED,DEPT PHARMACOL,PHILADELPHIA,PA 19104. NIMH,ST ELIZABETHS HOSP,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT PATHOL NEUROPATHOL,BOSTON,MA 02114. RP JOYCE, JN (reprint author), UNIV PENN,SCH MED,DEPT PSYCHIAT,CHEM NEUROANAT LAB,127 CLIN RES BLDG,422 CURRIE BLVD,PHILADELPHIA,PA 19104, USA. FU NIMH NIH HHS [MH 00044, MH 43852, MH 43880] NR 61 TC 59 Z9 60 U1 2 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-4476 J9 SYNAPSE JI Synapse PD MAR PY 1992 VL 10 IS 3 BP 228 EP 246 DI 10.1002/syn.890100306 PG 19 WC Neurosciences SC Neurosciences & Neurology GA HF843 UT WOS:A1992HF84300005 PM 1313605 ER PT J AU ELLWEIN, LB COHEN, SM AF ELLWEIN, LB COHEN, SM TI SIMULATION MODELING OF CARCINOGENESIS SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID RISK ASSESSMENT; BLADDER-CANCER; RAT; DYNAMICS; LIVER C1 UNIV NEBRASKA,MED CTR,DEPT PATHOL & MICROBIOL,OMAHA,NE 68198. RP ELLWEIN, LB (reprint author), NEI,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA44886, CA32513, CA36727] NR 19 TC 12 Z9 12 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR PY 1992 VL 113 IS 1 BP 98 EP 108 DI 10.1016/0041-008X(92)90013-I PG 11 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA HK779 UT WOS:A1992HK77900012 PM 1553760 ER PT J AU TORAASON, M BOHRMAN, JS KRIEG, E COMBES, RD WILLINGTON, SE ZAJAC, W LANGENBACH, R AF TORAASON, M BOHRMAN, JS KRIEG, E COMBES, RD WILLINGTON, SE ZAJAC, W LANGENBACH, R TI EVALUATION OF THE V79 CELL METABOLIC COOPERATION ASSAY AS A SCREEN INVITRO FOR DEVELOPMENTAL TOXICANTS SO TOXICOLOGY IN VITRO LA English DT Article ID GAP-JUNCTIONAL COMMUNICATION; INTERCELLULAR COMMUNICATION; INHIBITION; COOPERATION; TERATOGENS; VALIDATION; GROWTH; AGENTS; LINES AB Inhibition of intercellular communication is proposed to be one of several possible mechanisms of teratogenesis. 38 coded compounds were tested for their effect on intercellular communication in the V79 cell metabolic co-operation assay. Test chemicals were selected from a list of 47 agents recommended for the evaluation of assays in vitro for developmental toxicants. In addition to testing the effects of chemicals on intercellular communication, a separate cytotoxicity assay determined the concentration of each chemical that inhibited clonal expansion of V79 cells. Seven of the 29 designated teratogens were positive for inhibition of intercellular communication in the V79 assay. Additionally, four teratogens and one non-teratogen inhibited intercellular communication at only a single concentration or at cytotoxic concentrations and were scored as equivocal. Therefore, the sensitivity of the V79 assay for teratogens was 24% (seven of 29 teratogens tested positive), or 38% if the four equivocal chemicals are considered positive. None of the nine non-teratogens unequivocally inhibited intercellular communication, resulting in a specificity of 100%, which decreased to 89% when the single equivocal score was considered positive. The overall accuracy for correctly identifying teratogens and non-teratogens was 42% when equivocal chemicals were considered negative, and 50% if they were considered positive in the V79 assay. The results demonstrate that despite relatively low accuracy regarding a diverse group of developmental toxicants, chemicals that did inhibit intercellular communication under the present conditions had a high probability of being a teratogen. The low accuracy reported here contrasts with earlier reports on the assay and possible reasons for this are discussed. C1 INVERESK RES INT LTD,MUSSELBURGH EH21 7UB,SCOTLAND. NIEHS,RES TRIANGLE PK,NC 27709. RP TORAASON, M (reprint author), NIOSH,CELLULAR TOXICOL SECT C23,DBBS,CDC,4676 COLUMBIA PKWY,CINCINNATI,OH 45226, USA. NR 40 TC 4 Z9 4 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0887-2333 J9 TOXICOL IN VITRO JI Toxicol. Vitro PD MAR PY 1992 VL 6 IS 2 BP 165 EP 174 DI 10.1016/0887-2333(92)90011-F PG 10 WC Toxicology SC Toxicology GA HL185 UT WOS:A1992HL18500011 PM 20732108 ER PT J AU ROTH, SY ALLIS, CD AF ROTH, SY ALLIS, CD TI CHROMATIN CONDENSATION - DOES HISTONE H1 DEPHOSPHORYLATION PLAY A ROLE SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Article ID SEA-URCHIN; PHOSPHORYLATION; CELLS; TRANSCRIPTION; NUCLEOSOME; KINASE; SITES; H-1; H5 AB In this article we describe three distinct biological systems where histone H1 phosphorylation is uncoupled from mitosis and highly condensed chromatin is enriched in dephosphorylated forms of H1: the amitotic macronucleus of Tetrahymena, terminally differentiated avian erythrocytes and sea urchin sperm. Each system offers informative contrasts to the idea that H1 hyperphosphorylation is causally related to mitotic chromosome condensation. Assuming that higher order chromatin folding is primarily driven by electrostatic interactions between H1 and DNA, an alternative model is presented for the role of H1 phosphorylation in chromatin condensation. C1 SYRACUSE UNIV,DEPT BIOL,SYRACUSE,NY 13244. RP ROTH, SY (reprint author), NIDDK,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892, USA. NR 37 TC 214 Z9 217 U1 0 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD MAR PY 1992 VL 17 IS 3 BP 93 EP 98 DI 10.1016/0968-0004(92)90243-3 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HJ905 UT WOS:A1992HJ90500003 PM 1412698 ER PT J AU DUBNER, R RUDA, MA AF DUBNER, R RUDA, MA TI ACTIVITY-DEPENDENT NEURONAL PLASTICITY FOLLOWING TISSUE-INJURY AND INFLAMMATION SO TRENDS IN NEUROSCIENCES LA English DT Review ID RAT SPINAL-CORD; DORSAL HORN NEURONS; GENE-RELATED PEPTIDE; INSITU HYBRIDIZATION HISTOCHEMISTRY; ADJUVANT-INDUCED INFLAMMATION; MULTIPLE OPIOID SYSTEMS; IMMEDIATE-EARLY GENES; FOS-LIKE PROTEIN; C-FOS; PERIPHERAL INFLAMMATION AB Increases in neuronal activity in response to tissue injury lead to changes in gene expression and prolonged changes in the nervous system. These functional changes appear to contribute to the hyperalgesia and spontaneous pain associated with tissue injury. This activity-dependent plasticity involves neuropeptides, such as dynorphin, substance P and calcitonin gene-related peptide, and excitatory amino acids, such as NMDA, which are chemical mediators involved in nociceptive processing. Unilateral inflammation in the hindpaw of the rat results in an increase in the expression of preprodynorphin and preproenkephalin mRNA in the spinal cord, which parallels the behavioral hyperalgesia associated with the inflammation. Cellular intermediate-early genes, such as c-fos, are also expressed in spinal cord neurons following inflammation and activation of nociceptors. Peripheral inflammation results in an enlargement of the receptive fields of many of these neurons. Dynorphin applied to the spinal cord also induces an enlargement of receptive fields. NMDA antagonists block the hyperexcitability produced by inflammation. A model has been proposed in which dynorphin, substance P and calcitonin gene-related peptide enhance excitability at NMDA receptor sites, leading first to dorsal horn hyperexcitability and then to excessive depolarization and excitotoxicity. RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 59 TC 788 Z9 802 U1 4 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0166-2236 J9 TRENDS NEUROSCI JI Trends Neurosci. PD MAR PY 1992 VL 15 IS 3 BP 96 EP 103 DI 10.1016/0166-2236(92)90019-5 PG 8 WC Neurosciences SC Neurosciences & Neurology GA HF634 UT WOS:A1992HF63400007 PM 1373925 ER PT J AU YU, G FELSTED, RL AF YU, G FELSTED, RL TI EFFECT OF MYRISTOYLATION ON P27NEF SUBCELLULAR-DISTRIBUTION AND SUPPRESSION OF HIV-LTR TRANSCRIPTION SO VIROLOGY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; LONG TERMINAL REPEAT; PROTEIN ALPHA-SUBUNIT; MURINE LEUKEMIA-VIRUS; TRANS-ACTIVATOR GENE; NEF-PROTEIN; MUTATIONAL ANALYSIS; HTLV-III; T-CELLS; TYPE-1 RP YU, G (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,BIOL CHEM LAB,BLDG 37,ROOM 5D02,BETHESDA,MD 20892, USA. NR 54 TC 91 Z9 92 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD MAR PY 1992 VL 187 IS 1 BP 46 EP 55 DI 10.1016/0042-6822(92)90293-X PG 10 WC Virology SC Virology GA HD628 UT WOS:A1992HD62800005 PM 1736544 ER PT J AU HALL, SL STOKES, A TIERNEY, EL LONDON, WT BELSHE, RB NEWMAN, FC MURPHY, BR AF HALL, SL STOKES, A TIERNEY, EL LONDON, WT BELSHE, RB NEWMAN, FC MURPHY, BR TI COLD-PASSAGED HUMAN PARAINFLUENZA TYPE-3 VIRUSES CONTAIN TS AND NON-TS MUTATIONS LEADING TO ATTENUATION IN RHESUS-MONKEYS SO VIRUS RESEARCH LA English DT Article; Proceedings Paper CT 8TH INTERNATIONAL CONF ON NEGATIVE STRAND VIRUSES CY SEP 15-20, 1991 CL CHARLESTON, SC DE PARAINFLUENZA TYPE-3 VIRUS; COLD-PASSAGE; TS MUTATION; RHESUS MONKEY; VACCINE ID PARA-INFLUENZA; VACCINES AB Cold-passaged (CP) mutants derived from the JS strain of wild type wt parainfluenza type 3 virus (PIV3) are being evaluated as candidate live virus vaccines. The wt virus was serially passaged 45 times at low temperature and mutant clones with the cold-adapted (CA), temperature-sensitive (ts), and attenuation (ATT) phenotypes were selected following passage levels 12, 18 and 45 (cp12, cp18, and cp45). The cp45 virus was more ts than the cp12 or cp18 mutants, although all 3 mutant viruses were clearly attenuated in rhesus monkeys compared to wild type virus. The mean peak titers of the cp12 and cp18 viruses administered by the intratracheal route were at least 6000-fold lower than JSwt in both the upper and lower respiratory tracts. The cp45 virus was not recovered from monkeys administered virus by the i.t. route alone; however, when the cp45 virus was administered by the intranasal route, it replicated in the upper respiratory tract to a level comparable to that of the cp12 and cp18 viruses, but continued to be markedly restricted in the lower respiratory tract. These data indicate that the cp12 and cp18 viruses contain predominantly non-ts attenuating mutations whereas the cp45 mutant has both non-ts and ts attenuating mutations. Each of the CP mutants induced a high level of resistance to wild type virus challenge. Also, the ATT phenotype of the cp12 and cp18 viruses as measured in rhesus monkeys was stable after replication in chimpanzees or humans, respectively, although the ts phenotype was not. Based on its greater level of temperature sensitivity in vitro and its greater degree of attenuation in rhesus monkeys, the cp45 virus appears to be the most promising vaccine candidate for humans. C1 ST LOUIS UNIV,SCH MED,DIV INFECT DIS,ST LOUIS,MO 63104. RP HALL, SL (reprint author), NIAID,INFECT DIS LAB,BETHESDA,MD 20892, USA. NR 13 TC 63 Z9 63 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD MAR PY 1992 VL 22 IS 3 BP 173 EP 184 DI 10.1016/0168-1702(92)90049-F PG 12 WC Virology SC Virology GA HM812 UT WOS:A1992HM81200001 PM 1320790 ER PT J AU JAMISON, KR WYATT, RJ AF JAMISON, KR WYATT, RJ TI VANGOGH,VINCENT ILLNESS SO BRITISH MEDICAL JOURNAL LA English DT Letter C1 NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. RP JAMISON, KR (reprint author), JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21205, USA. NR 6 TC 8 Z9 10 U1 0 U2 1 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0959-8138 J9 BRIT MED J JI Br. Med. J. PD FEB 29 PY 1992 VL 304 IS 6826 BP 577 EP 577 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA HG599 UT WOS:A1992HG59900070 PM 1559082 ER PT J AU TU, AS TENNANT, RW SPALDING, JW AF TU, AS TENNANT, RW SPALDING, JW TI MORPHOLOGICAL TRANSFORMATION OF SYRIAN-HAMSTER EMBRYO CELLS BY MEZEREIN SO CANCER LETTERS LA English DT Article DE MEZEREIN; SHE CLONAL TRANSFORMATION; TUMOR PROMOTER ID REDUCED BICARBONATE CONCENTRATION; TUMOR PROMOTERS; CODED CHEMICALS; PH; FIBROBLASTS; CULTURES; GROWTH; ASSAY AB Mezerein (MEZ) has been described as a weak complete tumor promoter but an effective stage II promoter in the mouse skin initiation-promotion tumor model. In this study MEZ produced a strong transformation response when tested under code in the Syrian hamster embryo (SHE) clonal morphological transformation assay. Using a standard 7-day exposure protocol designed to detect complete carcinogens, MEZ was active at non-toxic concentrations, producing a linear response between 0.3-10 ng/ml in a log-log plot of transformation activity versus concentration. These concentrations were in the same range as those which had been shown to elicit promotion activity in several in vitro cell culture systems. Our data suggest that the SHE assay has the ability to detect some tumor promoters under the same conditions used to test for complete carcinogens. The possibility that MEZ may possess in vivo carcinogenic activity cannot be excluded. C1 NIEHS,NATL TOXICOL PROGRAM,EXPTL CARCINOGENESIS & MUTAGENESIS BRANCH,RES TRIANGLE PK,NC 27709. FU NIEHS NIH HHS [N01-ES-65151] NR 26 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3835 J9 CANCER LETT JI Cancer Lett. PD FEB 29 PY 1992 VL 62 IS 2 BP 159 EP 165 DI 10.1016/0304-3835(92)90187-Z PG 7 WC Oncology SC Oncology GA HJ664 UT WOS:A1992HJ66400009 PM 1540943 ER PT J AU DORA, E HINES, K KUNOS, G MCLAUGHLIN, AC AF DORA, E HINES, K KUNOS, G MCLAUGHLIN, AC TI SIGNIFICANCE OF AN OPIATE MECHANISM IN THE ADJUSTMENT OF CEREBROCORTICAL OXYGEN-CONSUMPTION AND BLOOD-FLOW DURING HYPERCAPNIC STRESS SO BRAIN RESEARCH LA English DT Article DE CEREBRAL CORTEX; HYPERCAPNIA; CEREBRAL BLOOD FLOW; CEREBRAL OXYGEN CONSUMPTION; ADRENAL MEDULLA ENKEPHALIN ID CEREBRAL OXYGEN; CONSCIOUS RAT; PIAL VESSELS; NALOXONE; BRAIN; NORMOCAPNIA; STIMULATION; BICARBONATE; RECEPTORS; MU AB The role of adrenal medulla-derived enkephalins in the control of hypercapnic cerebrocortical blood flow (CBF) and oxygen consumption (CMRO2) was investigated in the ketamine anesthetized rat. Three experimental interventions were utilized: inhibition of opioid receptors with naloxone, decrease of adrenal enkephalin production with chronic adrenal medullectomy, and treatment of adrenal demedullated animals with the synthetic enkephalin analog, D-Ala2, N-Me-Phe4, Gly5-ol-enkephalin (DAGO). In intact, untreated animals hypercapnia increased CBF and CMRO2 by approximately 300 and 35%, respectively. Naloxone reduced the hypercapnic increase of CBF, and transformed the hypercapnic increase of CMRO2 into a decrease. The mid-points of the dose-response curves for (1)-naloxone and (d)-naloxone were 10-mu-g/kg and 100-mu-g/kg, respectively. Adrenal demedullation and treatment with (1)-naloxone (0.2 mg/kg) decreased the hypercapnic CBF and CMRO2 by approximately 50%. DAGO treatment of adrenal demedullated animals-restored the hypercapnic CBF and CMRO2 to values similar to those found in intact animals. These observations suggest that opioid peptides (most likely adrenal medulla-derived enkephalins) play a significant role in the regulation of CMRO2 and CBF during moderate hypercapnia. C1 NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,ROCKVILLE,MD 20852. SEMMELWEIS UNIV MED,DEPT PHYSIOL 2,H-1085 BUDAPEST 8,HUNGARY. RP DORA, E (reprint author), NIAAA,METAB & MOLEC BIOL LAB,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 33 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 28 PY 1992 VL 573 IS 2 BP 293 EP 298 DI 10.1016/0006-8993(92)90775-5 PG 6 WC Neurosciences SC Neurosciences & Neurology GA HH575 UT WOS:A1992HH57500015 PM 1504767 ER PT J AU ABE, R ISHIDA, Y YUI, K KATSUMATA, M CHUSED, TM AF ABE, R ISHIDA, Y YUI, K KATSUMATA, M CHUSED, TM TI T-CELL-RECEPTOR MEDIATED RECOGNITION OF SELF LIGAND INDUCES SIGNALING IN IMMATURE THYMOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,ROCKVILLE,MD 20852. UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1987 EP A1987 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102242 ER PT J AU ALLEN, JB WONG, HL COSTA, GL BIENKOWSKI, M WAHL, SM AF ALLEN, JB WONG, HL COSTA, GL BIENKOWSKI, M WAHL, SM TI SUPPRESSION OF INFLAMMATION AND TISSUE DESTRUCTION IN STREPTOCOCCAL CELL-WALL (SCW)-INDUCED ARTHRITIS BY IL-4 SO FASEB JOURNAL LA English DT Meeting Abstract C1 UPJOHN CO,KALAMAZOO,MI 49001. NIDR,LI,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2054 EP A2054 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102637 ER PT J AU ALLEY, MC PACULACOX, CM HURSEY, ML MAYO, JG AF ALLEY, MC PACULACOX, CM HURSEY, ML MAYO, JG TI SEMIAUTOMATED IMAGE-ANALYSIS OF MICROCULTURE TETRAZOLIUM ASSAYS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,DCT,DEV THERAPEUT PROGRAM,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1624 EP A1624 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100146 ER PT J AU ANASTASSIOU, ED PALIOGIANNI, F BALOW, JP YAMADA, H BOUMPAS, DT AF ANASTASSIOU, ED PALIOGIANNI, F BALOW, JP YAMADA, H BOUMPAS, DT TI CYCLIC-AMP REGULATION OF IL-2 AND IL-2R-ALPHA GENE-EXPRESSION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1981 EP A1981 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102207 ER PT J AU APASOV, S REDEGELD, F TAFFS, R SITKOVSKY, M AF APASOV, S REDEGELD, F TAFFS, R SITKOVSKY, M TI STUDIES OF CD3-CD8 PHOSPHOPROTEIN AND CD3-CD4 PHOSPHORPROTEIN COMPLEXES USING INVITRO IMMUNE-COMPLEX KINASE ASSAY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LI,BETHESDA,MD 20892. RI Redegeld, Frank/O-6534-2016 OI Redegeld, Frank/0000-0001-8830-7960 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1984 EP A1984 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102227 ER PT J AU ATKINSON, TP KALINER, MA HOHMAN, RJ AF ATKINSON, TP KALINER, MA HOHMAN, RJ TI IGE RECEPTOR-MEDIATED TRANSLOCATION OF PHOSPHOLIPASE-C GAMMA TO THE MEMBRANE OF RAT BASOPHILIC LEUKEMIA-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2056 EP A2056 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102648 ER PT J AU BARKER, KT CLAYSMITH, AP CIOCE, V SOBEL, ME AF BARKER, KT CLAYSMITH, AP CIOCE, V SOBEL, ME TI RIBONUCLEOTIDE REDUCTASE M2 MESSENGER-RNA LEVELS ARE INCREASED IN HUMAN COLON CARCINOMAS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1933 EP A1933 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101935 ER PT J AU BASTA, M STROBER, W AF BASTA, M STROBER, W TI SELECTIVE EXPRESSION OF RAG-2-RELATED OR RAG-2-RELATED GENE IN MOUSE PEYER PATCH B-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,MUCOSAL IMMUN SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1708 EP A1708 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100631 ER PT J AU BONOMO, A MATZINGER, P AF BONOMO, A MATZINGER, P TI THYMIC EPITHELIUM, TOLERANCE AND DELETION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LCMI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1703 EP A1703 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100601 ER PT J AU BOYD, LF KOZLOWSKI, S MARGULIES, DH AF BOYD, LF KOZLOWSKI, S MARGULIES, DH TI QUANTITATIVE-ANALYSIS OF BINDING OF A MURINE CYTOMEGALOVIRUS-DERIVED PEPTIDE TO A SOLUBLE ANALOG OF H-2LD SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1800 EP A1800 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101162 ER PT J AU BRANDES, ME CORCORAN, ML WAHL, LM WAHL, SM AF BRANDES, ME CORCORAN, ML WAHL, LM WAHL, SM TI TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) TRIGGERS TYROSINE KINASE-ACTIVITY IN MONOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1715 EP A1715 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100668 ER PT J AU BURNETT, V LAWTON, M PHILPOT, R AF BURNETT, V LAWTON, M PHILPOT, R TI CLONING AND SEQUENCING OF RABBIT FLAVIN-CONTAINING MONOOXYGENASES ID1 AND IE1 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1844 EP A1844 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101417 ER PT J AU CHANG, AC WADSWORTH, S HALVORSON, M COLIGAN, JE AF CHANG, AC WADSWORTH, S HALVORSON, M COLIGAN, JE TI DIFFERENTIALLY GLYCOSYLATED FORMS OF THE VLA INTEGRIN BETA CHAIN ARE EXPRESSED ON MURINE THYMOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1700 EP A1700 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100583 ER PT J AU CHOUCHANE, L BROWN, TJ KINDT, TJ AF CHOUCHANE, L BROWN, TJ KINDT, TJ TI MOLECULAR CHARACTERIZATION OF THE RABBIT MHC-DOB GENE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,BETHESDA,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1710 EP A1710 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100638 ER PT J AU COSTA, GL CORCORAN, M WAHL, LM BERGER, AE WAHL, SM AF COSTA, GL CORCORAN, M WAHL, LM BERGER, AE WAHL, SM TI TGF-BETA SEQUENTIALLY INDUCES INTERLEUKIN-1 (IL-1) AND INTERLEUKIN-1 RECEPTOR ANTAGONIST (IL-1RA) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. UPJOHN CO,KALAMAZOO,MI 49001. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1717 EP A1717 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100684 ER PT J AU COWLEN, MS GLASGOW, WC ELING, TE AF COWLEN, MS GLASGOW, WC ELING, TE TI REGULATION OF EPIDERMAL GROWTH-FACTOR (EGF)-DEPENDENT MITOGENESIS AND PROTOONCOGENE EXPRESSION BY 13-HYDROXYOCTADECADIENOIC ACID (HODE) AND PROSTAGLANDIN-E2 (PGE2) IN SYRIAN-HAMSTER EMBRYO (SHE) FIBROBLASTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1849 EP A1849 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101447 ER PT J AU COX, GW MULLET, D FERTEL, RH VARESIO, L AF COX, GW MULLET, D FERTEL, RH VARESIO, L TI L-ARGININE METABOLISM MEDIATES THE INTERLEUKIN-2-DEPENDENT TUMORICIDAL ACTIVITY OF MOUSE MACROPHAGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,BRMP,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. OHIO STATE UNIV,COLL MED,DEPT PHARMACOL,COLUMBUS,OH 43210. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1718 EP A1718 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100689 ER PT J AU CROSS, N VIEIRA, NE JOHNSON, L HILLMAN, L AF CROSS, N VIEIRA, NE JOHNSON, L HILLMAN, L TI TRUE FRACTIONAL CALCIUM-ABSORPTION (ALPHA) USING CA-42 AND CA-44 DURING PREGNANCY AND LACTATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MISSOURI,KANSAS CITY,MO 64110. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1945 EP A1945 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102004 ER PT J AU CUTLER, RG BLAKELY, SR GRUNDEL, E MITCHELL, GV AF CUTLER, RG BLAKELY, SR GRUNDEL, E MITCHELL, GV TI PLASMA ANTIOXIDANT VITAMINS-C AND VITAMIN-E AND BETA-CAROTENE IN DIETARY-RESTRICTED RHESUS-MONKEYS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. US FDA,WASHINGTON,DC 20204. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1953 EP A1953 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102049 ER PT J AU DEGUCHI, Y WILSON, GL KEHRL, JH AF DEGUCHI, Y WILSON, GL KEHRL, JH TI A DIVERGED HOMEOBOX GENE, HB24, IS INVOLVED IN THE PROLIFERATION AND LINEAGE COMMITMENT OF HEMATOPOIETIC PROGENITORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1887 EP A1887 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101662 ER PT J AU DIBRINO, M PARKER, K BIDDISON, B COLIGAN, JE AF DIBRINO, M PARKER, K BIDDISON, B COLIGAN, JE TI THE ISOLATION OF ENDOGENOUS PEPTIDES FROM HLA-A3 AND HLA-A2 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NINCDS,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1800 EP A1800 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101160 ER PT J AU DING, L SHEVACH, EM AF DING, L SHEVACH, EM TI IL-10 INHIBITS MITOGEN-INDUCED T-CELL ACTIVATION BY SELECTIVELY INHIBITING MACROPHAGE (M-PHI) COSTIMULATORY FUNCTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1694 EP A1694 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100547 ER PT J AU EHRHARDT, RO HARRIMAN, GR STROBER, W AF EHRHARDT, RO HARRIMAN, GR STROBER, W TI FUNCTIONAL UNRESPONSIVENESS OF PP IGA+ AND IGG+ PLN B-CELLS TO THYMUS-INDEPENDENT TYPE-I AND OR TYPE-II ANTIGENS - A POSSIBLE MECHANISM FOR MUCOSAL TOLERANCE INDUCTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,MUCOSAL IMMUN SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1708 EP A1708 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100628 ER PT J AU ESPINOZADELGADO, I LONGO, DL MUSSO, T TAN, T BOSCO, MC GUSELLA, GL VARESIO, L AF ESPINOZADELGADO, I LONGO, DL MUSSO, T TAN, T BOSCO, MC GUSELLA, GL VARESIO, L TI EXPRESSION AND REGULATION OF INTERLEUKIN-2 RECEPTOR SUBUNIT GENES IN MONONUCLEAR PHAGOCYTIC-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701. RI Bosco, Maria Carla/J-7928-2016; varesio, luigi/J-8261-2016 OI Bosco, Maria Carla/0000-0003-1857-7193; varesio, luigi/0000-0001-5659-2218 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1972 EP A1972 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102156 ER PT J AU FITTS, MG METZGER, DW MAGE, RG HENDERSHOT, LM AF FITTS, MG METZGER, DW MAGE, RG HENDERSHOT, LM TI THE RABBIT B-CELL ANTIGEN RECEPTOR - AT LEAST 5 ACCESSORY PROTEINS ARE THE COMPONENTS OF 2 DISTINCT IG COMPLEXES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, IMMUNOL LAB, BETHESDA, MD 20892 USA. ST JUDE CHILDRENS RES HOSP, DEPT IMMUNOL, MEMPHIS, TN 38105 USA. ST JUDE CHILDRENS RES HOSP, DEPT TUMOR CELL BIOL, MEMPHIS, TN 38105 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1704 EP A1704 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100604 ER PT J AU FOOPHILLIPS, M KOZAK, C PRINCIPATO, MA ABE, R AF FOOPHILLIPS, M KOZAK, C PRINCIPATO, MA ABE, R TI IDENTIFICATION OF MMTV PROVIRUSES INVOLVED IN THE CLONAL DELETION OF SELF-MLS NEW REACTIVE T-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. US FDA,DIV MICROBIOL,LAUREL,MD 20708. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1987 EP A1987 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102245 ER PT J AU FORMAN, MR YONG, L LANZA, E GRAUBARD, B BEECHER, G BROWN, E SMITH, J MANGELS, AR AF FORMAN, MR YONG, L LANZA, E GRAUBARD, B BEECHER, G BROWN, E SMITH, J MANGELS, AR TI DIETARY DETERMINANTS OF PLASMA CAROTENOIDS - APPLICATION OF A CAROTENOID FOOD COMPOSITION DATA-BASE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,DCPC,BELTSVILLE,MD 20705. USDA,BELTSVILLE HUMAN NUTR RES CTR,BELTSVILLE,MD 20705. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1792 EP A1792 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101114 ER PT J AU FRUMAN, DA KLEE, CB BIERER, BE BURAKOFF, SJ AF FRUMAN, DA KLEE, CB BIERER, BE BURAKOFF, SJ TI FK506 AND CSA INHIBIT CALCINEURIN PHOSPHATASE-ACTIVITY IN LYMPHOCYTES-T SO FASEB JOURNAL LA English DT Meeting Abstract C1 BRIGHAM & WOMENS HOSP,DANA FARBER CANC INST,DIV PEDIAT ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BOSTON,MA 02115. NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1692 EP A1692 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100538 ER PT J AU GAZZINELLI, RT OSWALD, IP JAMES, SL MORSE, H SHER, A AF GAZZINELLI, RT OSWALD, IP JAMES, SL MORSE, H SHER, A TI UP-REGULATION OF TH2 CYTOKINE RESPONSE INDUCED BY MURINE RETROVIRUS INFECTION - ASSOCIATION WITH DECREASED RESISTANCE TO TOXOPLASMA-GONDII SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LPD & LIP,BETHESDA,MD 20892. RI OSWALD, Isabelle/A-8497-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1688 EP A1688 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100515 ER PT J AU GOPALAKRISHNA, R GUNDIMEDA, U WILSON, J ANDERSON, WB CHEN, Z AF GOPALAKRISHNA, R GUNDIMEDA, U WILSON, J ANDERSON, WB CHEN, Z TI RAPID FILTRATION ASSAYS FOR PROTEIN-KINASE-C ACTIVITY AND PHORBOL ESTER BINDING USING MULTIWELL PLATES WITH FITTED FILTRATION DISKS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV SO CALIF,DEPT PHARMACOL & NUTR,LOS ANGELES,CA 90033. NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1853 EP A1853 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101471 ER PT J AU HAMAWY, MM OLIVER, C MERGENHAGEN, S SIRAGANIAN, RP AF HAMAWY, MM OLIVER, C MERGENHAGEN, S SIRAGANIAN, RP TI ADHERENCE TO FIBRONECTIN-COATED SURFACES ENHANCES SECRETION FROM RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1723 EP A1723 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100714 ER PT J AU HARIHARAN, K CARALLI, VM NORTON, FL NARA, P KANG, CY AF HARIHARAN, K CARALLI, VM NORTON, FL NARA, P KANG, CY TI ANALYSIS OF CROSS-REACTIVE ANTI-GP120 ANTIBODY POPULATION IN HIV+ SERA SO FASEB JOURNAL LA English DT Meeting Abstract C1 IDEC PHARMACEUT CORP,LA JOLLA,CA 92037. NCI,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1725 EP A1725 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100730 ER PT J AU HIRAMATSU, Y BAUM, BJ AMBUDKAR, IS AF HIRAMATSU, Y BAUM, BJ AMBUDKAR, IS TI ELEVATION OF [CA-2(+)] DUE TO INTRACELLULAR CA-2(+) RELEASE RETARDS CARBACHOL STIMULATION OF DIVALENT-CATION ENTRY IN RAT PAROTID-GLAND ACINAR-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,CIPCD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1761 EP A1761 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100937 ER PT J AU HONG, HL BOORMAN, GA AF HONG, HL BOORMAN, GA TI DEMONSTRATION OF RESIDUAL MYELOTOXICITY IN MICE EXPOSED TO LINDANE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NTP,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1912 EP A1912 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101811 ER PT J AU HONG, JX WILSON, GL KEHRL, JH AF HONG, JX WILSON, GL KEHRL, JH TI CHARACTERIZATION OF SUBTRACTED CLONE 34 - A NOVEL HUMAN LYMPHOCYTE-B ACTIVATION GENE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1989 EP A1989 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102258 ER PT J AU HOOK, WA BERENSTEIN, EH BADER, GM ABBASI, F SIRAGANIAN, RP AF HOOK, WA BERENSTEIN, EH BADER, GM ABBASI, F SIRAGANIAN, RP TI DIFFERENT MONOCLONAL ANTI-CD45 ANTIBODIES INHIBIT OR ENHANCE IGE-MEDIATED HISTAMINE-RELEASE FROM HUMAN BASOPHILS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2058 EP A2058 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102662 ER PT J AU HULTSCH, T HOHMAN, R AF HULTSCH, T HOHMAN, R TI THE EFFECT OF THE IMMUNOPHILIN LIGANDS RAPAMYCIN AND FK506 ON IL-3 DEPENDENT PROLIFERATION OF PT18-MAST CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2008 EP A2008 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102367 ER PT J AU HUNTER, M SUGIYAMA, H SITKOVSKY, M AF HUNTER, M SUGIYAMA, H SITKOVSKY, M TI EFFECT OF TRANSFECTION OF 2B4-T-HELPER HYBRIDOMA WITH CAMP BINDING SITE-MUTATED REGULATORY (R1M) SUBUNIT OF PK-A ON T-CELL RECEPTOR TRIGGERED PROGRAMMED CELL-DEATH SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1984 EP A1984 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102224 ER PT J AU IGARASHI, Y WHITE, M HAUSFELD, J IRANI, A SCHWARTZ, L KALINER, M AF IGARASHI, Y WHITE, M HAUSFELD, J IRANI, A SCHWARTZ, L KALINER, M TI IGE POSITIVE CELLS IN HUMAN NASAL-MUCOSA ARE MOSTLY MAST-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 WASHINGTON HOSP CTR,WASHINGTON,DC 20010. NIH,BETHESDA,MD 20892. VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,RICHMOND,VA 23298. NR 0 TC 4 Z9 4 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2005 EP A2005 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102351 ER PT J AU JOHNSTON, J LLOYD, A MICHIEL, D OPPENHEIM, J KELVIN, D AF JOHNSTON, J LLOYD, A MICHIEL, D OPPENHEIM, J KELVIN, D TI MOLECULAR-CLONING OF HOMOLOGOUS MEMBERS OF THE CHEMOTACTIC CYTOKINE RECEPTOR FAMILY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21701. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2054 EP A2054 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102636 ER PT J AU KANG, CY NARA, P CHAMAT, S CARALLI, V CHEN, A NGUYEN, ML YOSHIYMA, H MORROW, WJW HO, DD KOHLER, H AF KANG, CY NARA, P CHAMAT, S CARALLI, V CHEN, A NGUYEN, ML YOSHIYMA, H MORROW, WJW HO, DD KOHLER, H TI A MONOCLONAL ANTIIDIOTYPE ANTIBODY ELICITS BROADLY NEUTRALIZING ANTI-GP120 ANTIBODIES IN MONKEYS SO FASEB JOURNAL LA English DT Meeting Abstract C1 IDEC PHARMACEUT CORP,LA JOLLA,CA 92037. NCI,FREDERICK,MD 21701. AARON DIAMOND AIDS RES CTR,NEW YORK,NY 10016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1725 EP A1725 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100727 ER PT J AU KANT, AK SCHATZKIN, A ZIEGLER, RG AF KANT, AK SCHATZKIN, A ZIEGLER, RG TI DIETARY DIVERSITY AND SUBSEQUENT MORTALITY FROM CARDIOVASCULAR-DISEASE AND CANCER IN THE NHANES-I EPIDEMIOLOGIC FOLLOW-UP-STUDY SO FASEB JOURNAL LA English DT Meeting Abstract C1 CUNY QUEENS COLL,FLUSHING,NY 11367. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1682 EP A1682 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100477 ER PT J AU KARLHOFER, FM RIBAUDO, RK YOKOYAMA, WM AF KARLHOFER, FM RIBAUDO, RK YOKOYAMA, WM TI A NATURAL-KILLER (NK) CELL-RECEPTOR (LY-49), SPECIFIC FOR AN MHC CLASS-I ANTIGEN, REGULATES NK CELL LYTIC ACTIVITY SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF SAN FRANCISCO,ROS RUSSELL & ARTH RES LAB,DEPT MED,SAN FRANCISCO,CA 94143. NIH,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1691 EP A1691 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100528 ER PT J AU KAWATE, R TALAN, M ENGEL, B AF KAWATE, R TALAN, M ENGEL, B TI COLD-ACCLIMATION IN C57BL/6J MICE INVOLVES AN INCREASE IN SYMPATHETIC NERVOUS ACTIVITY TO INTERSCAPULAR BROWN ADIPOSE-TISSUE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1829 EP A1829 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101329 ER PT J AU KIM, YH BUCHHOLZ, MJ NORDIN, AA AF KIM, YH BUCHHOLZ, MJ NORDIN, AA TI A POSSIBLE CORRELATION BETWEEN CDC2 GENE-EXPRESSION AND THE FAILURE OF THE T-CELLS OF OLD MICE TO TRAVERSE G1/S SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1982 EP A1982 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102214 ER PT J AU KINZER, C KEEGAN, A PAUL, WE AF KINZER, C KEEGAN, A PAUL, WE TI A MAST-CELL SPECIFIC CELL-SURFACE PROTEIN, P161, NOT EXPRESSED ON BASOPHILS, IS DETECTED BY MONOCLONAL-ANTIBODY (MAB) K-1 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1724 EP A1724 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100723 ER PT J AU KOSSMANN, T MORGANTIKOSSMANN, MC MANISCHEWITZ, JF ORENSTEIN, JM MERGENHAGEN, SE WAHL, SM SMITH, PD AF KOSSMANN, T MORGANTIKOSSMANN, MC MANISCHEWITZ, JF ORENSTEIN, JM MERGENHAGEN, SE WAHL, SM SMITH, PD TI CYTOMEGALOVIRUS (CMV) INFECTED ASTROCYTES PRODUCE TGF-BETA - POTENTIAL ROLE IN CMV ENCEPHALOPATHY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,IMMUNOL LAB,BETHESDA,MD 20892. US FDA,CTR BIOL EVALUAT & RES,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,DEPT PATHOL,WASHINGTON,DC 20037. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1608 EP A1608 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100047 ER PT J AU KOZLOW, EJ WILSON, GL KEHRL, JH AF KOZLOW, EJ WILSON, GL KEHRL, JH TI ISOLATION AND CHARACTERIZATION OF 2 GENES WHICH ARE EXPRESSED AT HIGH-LEVELS IN LYMPHOCYTES-B SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1990 EP A1990 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102262 ER PT J AU KRAMLIK, SK ALTEMUS, M CASTONGUAY, TW AF KRAMLIK, SK ALTEMUS, M CASTONGUAY, TW TI EFFECTS OF THE ACUTE ADMINISTRATION OF RU-486 ON DIETARY-FAT PREFERENCE IN FASTED LEAN AND OBESE MEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MARYLAND,COLLEGE PK,MD 20742. NIMH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1675 EP A1675 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100440 ER PT J AU LAM, JS CULVER, KW SHU, S BLAESE, RM FOX, BA AF LAM, JS CULVER, KW SHU, S BLAESE, RM FOX, BA TI TRANSDUCTION OF HUMAN IL-1-BETA GENE INTO A T-CELL CLONE IS ASSOCIATED WITH AN ALTERATION IN TNF SECRETION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,METAB BRANCH,BETHESDA,MD 20892. UNIV MICHIGAN,DEPT SURG,ANN ARBOR,MI 48109. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1998 EP A1998 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102309 ER PT J AU LEE, NH FRASER, CM AF LEE, NH FRASER, CM TI POSTTRANSCRIPTIONAL REGULATION OF THE M1 MUSCARINIC ACETYLCHOLINE-RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,ADAMHA,NEUROGENET LAB,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1861 EP A1861 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101512 ER PT J AU LEWIS, KC WANG, TTY PHANG, JM AF LEWIS, KC WANG, TTY PHANG, JM TI EXPRESSION OF HUMAN PLASMA RETINOL-BINDING PROTEIN IN ESCHERICHIA-COLI SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,NUTR & MOLEC REGULAT LAB,FREDERICK,MD 21702. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1658 EP A1658 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100340 ER PT J AU LOCKWICH, T BAUM, BJ AMBUDKAR, IS AF LOCKWICH, T BAUM, BJ AMBUDKAR, IS TI CHARACTERIZATION OF CA-2(+) FLUXES IN RAT PAROTID-GLAND ACINAR BASOLATERAL PLASMA-MEMBRANES - EFFECTS OF CARBOXYL REAGENTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,CIPCD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1761 EP A1761 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100935 ER PT J AU LORENS, SA GEORGE, M PETROVIC, L DERSCH, C CLANCY, J ZACZEK, R AF LORENS, SA GEORGE, M PETROVIC, L DERSCH, C CLANCY, J ZACZEK, R TI AGE-DEPENDENT EFFECTS OF D-FENFLURAMINE (D-FEN) ON SPLENIC MONOAMINES AND IMMUNE FUNCTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 LOYOLA UNIV,MED CTR,DEPT PHARMACOL,MAYWOOD,IL 60153. LOYOLA UNIV,MED CTR,DEPT ANAT,MAYWOOD,IL 60153. NIDA,AGR RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1875 EP A1875 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101592 ER PT J AU LYNCH, F SHEVACH, EM AF LYNCH, F SHEVACH, EM TI ACTIVATION REQUIREMENTS OF THYMIC GAMMA-DELTA T-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1695 EP A1695 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100552 ER PT J AU MAITY, R MUKHERJEE, R SKOLNICK, P ARORA, PK AF MAITY, R MUKHERJEE, R SKOLNICK, P ARORA, PK TI MORPHINE MODULATES ALLOREACTIVITY INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,NEUROSCI LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2008 EP A2008 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102365 ER PT J AU MANGAN, DF WAHL, SM AF MANGAN, DF WAHL, SM TI IL-4 ENHANCES PROGRAMMED CELL-DEATH (APOPTOSIS) IN STIMULATED HUMAN MONOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1616 EP A1616 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100096 ER PT J AU MARIC, I MARIC, D CHEN, HC BARKER, JL AF MARIC, I MARIC, D CHEN, HC BARKER, JL TI CYTOTOXIC ACTIVITY OF MAGAININ G DEPENDS ON CELL-MEMBRANE POTENTIAL SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,NEUROPHYSIOL LAB,BETHESDA,MD 20892. NICHHD,ENDOCRINOL & REPROD RES BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1930 EP A1930 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101915 ER PT J AU MCCANN, DJ SU, TP AF MCCANN, DJ SU, TP TI TRIS INHIBITS THE BINDING OF (+)[3H]SKF-10,047 TO DELTA RECEPTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA ADDICT RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1881 EP A1881 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101631 ER PT J AU MELILLO, G COX, GW WANG, JM VARESIO, L AF MELILLO, G COX, GW WANG, JM VARESIO, L TI INTERLEUKIN-2 AUGMENTS JE MESSENGER-RNA EXPRESSION AND CHEMOTACTIC ACTIVITY IN MOUSE MACROPHAGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,BRMP,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. RI varesio, luigi/J-8261-2016 OI varesio, luigi/0000-0001-5659-2218 NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1719 EP A1719 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100696 ER PT J AU MILLER, DS PRITCHARD, JB AF MILLER, DS PRITCHARD, JB TI INTRACELLULAR SEQUESTRATION OF AN ORGANIC ANION, FLUORESCEIN (FL) IN RENAL-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1809 EP A1809 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101213 ER PT J AU MORENO, B TITUS, J WINKLER, D SEGAL, D WUNDERLICH, J AF MORENO, B TITUS, J WINKLER, D SEGAL, D WUNDERLICH, J TI RETARGETING MURINE T-CELLS TO REACT AGAINST MURINE BREAST-CANCER CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2059 EP A2059 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102666 ER PT J AU MORGANTIKOSSMANN, MC KOSSMANN, T BRANDES, ME MERGENHAGEN, SE TRENTZ, O WAHL, SM AF MORGANTIKOSSMANN, MC KOSSMANN, T BRANDES, ME MERGENHAGEN, SE TRENTZ, O WAHL, SM TI ASTROCYTES PRODUCE TGF-BETA WHICH REGULATES ASTROCYTE MIGRATION AND PROLIFERATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV HOSP ZURICH,CH-8091 ZURICH,SWITZERLAND. NIDR,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2061 EP A2061 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102678 ER PT J AU MULRONEY, SE LEROITH, D LUMPKIN, MD ROBERTS, CT HARAMATI, A AF MULRONEY, SE LEROITH, D LUMPKIN, MD ROBERTS, CT HARAMATI, A TI AGE-DEPENDENT DIFFERENCES IN COMPENSATORY RENAL GROWTH FOLLOWING UNILATERAL NEPHRECTOMY - ROLE OF GH AND IGF-I SO FASEB JOURNAL LA English DT Meeting Abstract C1 GEORGETOWN UNIV,SCH MED,WASHINGTON,DC. NIDDK,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1744 EP A1744 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100836 ER PT J AU NAKAYAMA, T UEDA, Y YAMADA, H SHORES, EW SINGER, A JUNE, CH AF NAKAYAMA, T UEDA, Y YAMADA, H SHORES, EW SINGER, A JUNE, CH TI INSITU ELEVATION OF [CA-2+]I IN THYMOCYTES RESIDENT IN A NEGATIVE SELECTING THYMUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NMRI,IMMUNE CELL BIOL PROGRAM,BETHESDA,MD 20814. NCI,IMMUNOL BRANCH,BETHESDA,MD 20892. RI Nakayama, Toshinori/E-1067-2017 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1977 EP A1977 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102187 ER PT J AU OBRIEN, K VICCHIO, D ALLEN, L YERGEY, A RAY, R HOLICK, M AF OBRIEN, K VICCHIO, D ALLEN, L YERGEY, A RAY, R HOLICK, M TI THE SERUM HALF-LIFE OF DEUTERATED 25-HYDROXYVITAMIN-D3 APPEARS TO BE SHORTER THAN PREVIOUSLY REPORTED SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CONNECTICUT,STORRS,CT 06269. BOSTON UNIV,BOSTON,MA 02118. NICHHD,LTPB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1955 EP A1955 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102064 ER PT J AU OSWALD, IP WYNN, TA GAZZINELLI, RT SHER, A JAMES, SL AF OSWALD, IP WYNN, TA GAZZINELLI, RT SHER, A JAMES, SL TI IL-10 INHIBITS MACROPHAGE MICROBICIDAL ACTIVITY BY BLOCKING THE PRODUCTION OF TNF-ALPHA REQUIRED FOR THE TRIGGERING STEP OF IFN-GAMMA INDUCED ACTIVATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL & CELL BIOL SECT,LPD,BETHESDA,MD 20892. RI Wynn, Thomas/C-2797-2011; OSWALD, Isabelle/A-8497-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1688 EP A1688 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100511 ER PT J AU OZAWA, K SZALLASI, Z BLUMBERG, PM BEAVEN, MA AF OZAWA, K SZALLASI, Z BLUMBERG, PM BEAVEN, MA TI THE ROLE OF CA2+-INDEPENDENT ISOZYMES OF PROTEIN-KINASE-C (PKC) IN EXOCYTOSIS IN ANTIGEN-STIMULATED RAT BASOPHIL RBL-2H3 CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1633 EP A1633 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100195 ER PT J AU PALIOGIANNI, F RAPTIS, A BALOW, JE BOUMPAS, DT AF PALIOGIANNI, F RAPTIS, A BALOW, JE BOUMPAS, DT TI DIFFERENTIAL-EFFECTS OF GLUCOCORTICOIDS AND CYCLOSPORINE A ON BINDING OF NUCLEAR FACTORS TO THE IL-2 GENE ENHANCER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2008 EP A2009 PN 2 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102370 ER PT J AU PARDHASARADHI, K KUTTY, RK BERTOLOTTI, R KRISHNA, G AF PARDHASARADHI, K KUTTY, RK BERTOLOTTI, R KRISHNA, G TI DETECTION OF THE EXPRESSION OF ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR TYPE A (ANPRA) GENE IN HUMAN LIVER BY RNA-PCR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1623 EP A1623 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100136 ER PT J AU PARKER, K BEDNAREK, M CARRENO, B DIBRINO, M ZWEERINK, H BIDDISON, W COLIGAN, J AF PARKER, K BEDNAREK, M CARRENO, B DIBRINO, M ZWEERINK, H BIDDISON, W COLIGAN, J TI BINDING OF PEPTIDES AND BETA-2-MICROGLOBULIN TO HLA CLASS I MOLECULES INVITRO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NINCDS,BETHESDA,MD 20892. MERCK SHARP & DOHME LTD,RAHWAY,NJ 07065. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1800 EP A1800 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101158 ER PT J AU PASH, JM BUSTIN, M AF PASH, JM BUSTIN, M TI EXPRESSION OF HUMAN CHROMOSOMAL PROTEIN HMG-14 INHIBITS FUSION OF MOUSE MYOBLASTS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RI Bustin, Michael/G-6155-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1937 EP A1937 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101957 ER PT J AU POISNER, AM SUH, HH POISNER, R HUDSON, P HONG, JS AF POISNER, AM SUH, HH POISNER, R HUDSON, P HONG, JS TI STIMULATION OF RENIN AND PRORENIN RELEASE FROM ADRENAL-MEDULLARY CELLS BY PGE2 AND FORSKOLIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV KANSAS,MED CTR,DEPT PHARMACOL,KANSAS CITY,KS 66160. NIEHS,LMIN,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1810 EP A1810 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101216 ER PT J AU RABINOVITZ, M AF RABINOVITZ, M TI THE PLEIOTYPIC RESPONSE TO AMINO-ACID DEFICIENCY IS THE RESULT OF INTERACTION BETWEEN COMPONENTS OF PATHWAYS OF PROTEIN-SYNTHESIS AND GLYCOLYSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,DCT,DTP,MOL PHARMACOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1919 EP A1919 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101853 ER PT J AU REDEGELD, FA CHATTERJEE, S BERGER, NA SITKOVSKY, MV AF REDEGELD, FA CHATTERJEE, S BERGER, NA SITKOVSKY, MV TI POLY-(ADP-RIBOSE) POLYMERASE PARTIALLY CONTRIBUTES TO TARGET-CELL DEATH TRIGGERED BY CYTOLYTIC T LYMPHOCYTES (CTL) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. RI Redegeld, Frank/O-6534-2016 OI Redegeld, Frank/0000-0001-8830-7960 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1793 EP A1793 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101120 ER PT J AU RIGATTO, H FITZGERALD, S WILLIS, M YU, C AF RIGATTO, H FITZGERALD, S WILLIS, M YU, C TI IN SEARCH OF THE RESPIRATORY CENTER - CULTURE OF MEDULLARY FETAL CELLS SENSITIVE TO CO2 AND LOW PH SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MANITOBA,WINNIPEG R3T 2N2,MANITOBA,CANADA. NIH,DEPT PEDIAT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1825 EP A1825 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101306 ER PT J AU ROCKEN, M SHEVACH, EM AF ROCKEN, M SHEVACH, EM TI CD4+ T-CELLS FROM ANIMALS TOLERIZED TO STAPHYLOCOCCAL-ENTEROTOXIN B(SEB) CAN ACT AS TH2 EFFECTOR-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1699 EP A1699 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100578 ER PT J AU ROILIDES, E UHLIG, K VENZON, D PIZZO, PA WALSH, T AF ROILIDES, E UHLIG, K VENZON, D PIZZO, PA WALSH, T TI G-CSF AND IFN-GAMMA PREVENT CORTICOSTEROID (C)-INDUCED SUPPRESSION OF HUMAN POLYMORPHONUCLEAR LEUKOCYTE (PMN) OXIDATIVE RESPONSES AND DAMAGE OF ASPERGILLUS HYPHAE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,PEDIAT BRANCH,BETHESDA,MD 20892. NCI,BIOSTAT SECT,BETHESDA,MD 20892. RI Venzon, David/B-3078-2008 NR 0 TC 4 Z9 4 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2000 EP A2000 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102320 ER PT J AU ROSS, SA AHRENS, RA DE LUCA, LM AF ROSS, SA AHRENS, RA DE LUCA, LM TI RETINOIC ACID ENHANCES CELL-ADHESION AND LAMININ BIOSYNTHESIS DURING F9 CELL-DIFFERENTIATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MARYLAND, NUTR SCI PROGRAM, COLLEGE PK, MD 20742 USA. NCI, CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB, BETHESDA, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1783 EP A1783 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101062 ER PT J AU ROTTEM, M METCALFE, DD AF ROTTEM, M METCALFE, DD TI FC-EPSILON-RI EXPRESSION ON MAST-CELLS IS A MARKER OF TERMINAL DIFFERENTIATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1723 EP A1723 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100719 ER PT J AU RUTTNER, Z LIGETI, L REINLIB, L HINES, K MCLAUGHLIN, AC AF RUTTNER, Z LIGETI, L REINLIB, L HINES, K MCLAUGHLIN, AC TI SURFACE FLUOROMETRY OF INDO LOADED ISOLATED PERFUSED-RAT-LIVER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NATL INST ALCOHOL ABUSE & ALCOHOLISM,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1761 EP A1761 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100936 ER PT J AU SALOMON, DR GRALNICK, HR SHEVACH, EM AF SALOMON, DR GRALNICK, HR SHEVACH, EM TI FIBRINOGEN FUNCTIONS AS A COSTIMULATORY MOLECULE FOR HUMAN T-CELL ACTIVATION BY INTERACTING WITH VLA-5 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LI,BETHESDA,MD 20892. NIH,CTR CLIN,BETHESDA,MD 20892. RI Salomon, Daniel/E-9380-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1991 EP A1991 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102265 ER PT J AU SAWASDIKOSOL, S SIMPSON, RM ZHAO, TM HAGUE, BF BOWERS, F ROBINSON, MA KINDT, TJ AF SAWASDIKOSOL, S SIMPSON, RM ZHAO, TM HAGUE, BF BOWERS, F ROBINSON, MA KINDT, TJ TI ACUTE MONONUCLEAR CELL LEUKEMIA IN OUTBRED RABBITS INOCULATED WITH HTLV-I TRANSFORMED RABBIT-CELL LINES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2009 EP A2009 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102372 ER PT J AU SEI, Y SKOLNICK, P ARORA, PK AF SEI, Y SKOLNICK, P ARORA, PK TI DUAL COLOR ANALYSIS OF CALCIUM SIGNALING IN LYMPHOCYTES AND ITS APPLICATION (STRESS, OPIOID, MAIDS AND AGING) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,NEUROSCI LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1727 EP A1727 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100742 ER PT J AU SHEARS, S ABDULLAH, M HUGHES, P MENNITI, F MARECEK, J PRESTWICH, G AF SHEARS, S ABDULLAH, M HUGHES, P MENNITI, F MARECEK, J PRESTWICH, G TI PURIFICATION OF (1,3,4)IP3 6-KINASE AND (3,4,5,6)IP4 1-KINASE FROM RAT-LIVER HOMOGENATES USING AN IP6-AFFINITY RESIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,LCMP,RES TRIANGLE PK,NC 27709. SUNY STONY BROOK,DEPT CHEM,STONY BROOK,NY 11794. NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1923 EP A1923 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101875 ER PT J AU SHER, A HIENY, S BANCROFT, G GAZZINELLI, RT OSWALD, IP AF SHER, A HIENY, S BANCROFT, G GAZZINELLI, RT OSWALD, IP TI T-INDEPENDENT INDUCTION OF IFN-GAMMA AND TNF-ALPHA BY TOXOPLASMA-GONDII SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. LONDON SCH TROP MED & HYG,LONDON WC1E 7HT,ENGLAND. RI OSWALD, Isabelle/A-8497-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1691 EP A1691 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100531 ER PT J AU SHINYA, E MCDONAGH, KT NEY, P SHIMADA, T AF SHINYA, E MCDONAGH, KT NEY, P SHIMADA, T TI A COMMON NUCLEAR FACTOR BINDS TO THE INITIATOR ELEMENTS OF THE HUMAN DIHYDROFOLATE-REDUCTASE (DHFR) AND MISMATCH REPAIR PROTEIN-1 (MRP1) GENES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. NIPPON MED COLL,DEPT BIOCHEM & MOLEC BIOL,TOKYO 113,JAPAN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1644 EP A1644 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100259 ER PT J AU SMITH, JJ SYKES, DB MILLER, DS AF SMITH, JJ SYKES, DB MILLER, DS TI XENOPUS-LAEVIS OOCYTES, A MODEL SYSTEM TO INVESTIGATE SIGNAL TRANSDUCTION BY GROWTH-HORMONE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1637 EP A1637 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100220 ER PT J AU SPRAUL, M ANDERSON, EA BOGARDUS, C RAVUSSIN, E AF SPRAUL, M ANDERSON, EA BOGARDUS, C RAVUSSIN, E TI LOWER MUSCLE SYMPATHETIC NERVOUS ACTIVITY (MSNA) IN PIMA-INDIANS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN DIABETES & NUTR SECT,PHOENIX,AZ 85016. UNIV IOWA,IOWA CITY,IA 52242. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1647 EP A1647 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100276 ER PT J AU SUGIYAMA, H CHEN, P PIPER, M SITKOVSKY, M AF SUGIYAMA, H CHEN, P PIPER, M SITKOVSKY, M TI EFFECT OF ANTIMESSENGER OLIGODEOXYNUCLEOTIDES TO THE CAMP-DEPENDENT PROTEIN-KINASE (PKA) CATALYTIC SUBUNIT ON EFFECTOR FUNCTIONS OF CYTOLYTIC LYMPHOCYTES-T (CTL) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1984 EP A1984 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102226 ER PT J AU THEODOS, CM SACKS, DL MCMASTER, WR TITUS, RG AF THEODOS, CM SACKS, DL MCMASTER, WR TITUS, RG TI ANALYSIS OF THE ANTIGENIC SPECIFICITY OF T-CELL CLONES DERIVED FROM INFECTED, GENETICALLY-RESISTANT C3H MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. NIAID,BETHESDA,MD 20892. UNIV BRITISH COLUMBIA,VANCOUVER V6T 1W5,BC,CANADA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1797 EP A1797 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101142 ER PT J AU THEVENIN, C KOZLOW, EJ WILSON, GL KEHRL, JH AF THEVENIN, C KOZLOW, EJ WILSON, GL KEHRL, JH TI IDENTIFICATION OF DIVERGED OCTAMER BINDING-SITES IN THE LYMPHOCYTE-B SPECIFIC PROMOTERS CD20 AND CD21 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1989 EP A1989 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102256 ER PT J AU TRAN, AC KANG, SM BEVERLY, B GRILLI, M BRORSON, B SCHWARTZ, R LENARDO, MJ AF TRAN, AC KANG, SM BEVERLY, B GRILLI, M BRORSON, B SCHWARTZ, R LENARDO, MJ TI INTERLEUKIN-2 GENE-REGULATION IN NONTRANSFORMED CD4+ LYMPHOCYTE-T CLONES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,LCMI,BETHESDA,MD 20892. HOWARD HUGHES MED INST,COCONUT GROVE,FL 33133. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1982 EP A1982 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102215 ER PT J AU URBAN, J MADDEN, K CHEEVER, A TROTTA, P KATONA, I FINKELMAN, F AF URBAN, J MADDEN, K CHEEVER, A TROTTA, P KATONA, I FINKELMAN, F TI IFN-A/B AND IFN-G INHIBIT HOST IMMUNE AND INFLAMMATORY RESPONSES IN MICE INFECTED WITH NIPPOSTRONGYLUS-BRASILIENSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS,BELTSVILLE AGR RES CTR,HDL,BELTSVILLE,MD 20705. NIH,LPD,USUHS,BETHESDA,MD 20892. SCHERING RES,BLOOMFIELD,NJ. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1688 EP A1688 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100514 ER PT J AU VOSTAL, JG SHULMAN, NR AF VOSTAL, JG SHULMAN, NR TI CYTOSOLIC AND STORED CALCIUM ANTAGONISTICALLY CONTROL TYROSINE PHOSPHORYLATION OF VINCULIN IN PLATELETS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1761 EP A1761 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100938 ER PT J AU WANIDWORANUN, C JAMES, SP STROBER, W AF WANIDWORANUN, C JAMES, SP STROBER, W TI CYTOKINE REGULATION OF THE EXPRESSION OF VIL-10 IN EPSTEIN-BARR VIRUS-TRANSFORMED B-LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,MUCOSAL IMMUN SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1895 EP A1895 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101713 ER PT J AU WINKLER, BS ORSELLI, SM FLETCHER, RT CHADER, GJ AF WINKLER, BS ORSELLI, SM FLETCHER, RT CHADER, GJ TI ANAEROBIC GLYCOLYSIS SUPPORTS HIGH (DARK-ADAPTED) CONCENTRATION OF CYCLIC-GMP AND PHOTOTRANSDUCTION IN RAT RETINA SO FASEB JOURNAL LA English DT Meeting Abstract C1 OAKLAND UNIV,EYE RES INST,ROCHESTER,MI 48309. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2027 EP A2027 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102480 ER PT J AU WONG, HL COSTA, GL LOTZE, MT WAHL, SM AF WONG, HL COSTA, GL LOTZE, MT WAHL, SM TI IL-4 UP-REGULATES EXPRESSION OF IL-1 RECEPTOR ANTAGONIST BY HUMAN PERIPHERAL-BLOOD MONOCYTES INVITRO AND INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,HLI,BETHESDA,MD 20892. UNIV PITTSBURGH,PITTSBURGH CANC INST,PITTSBURGH,PA 15261. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A2055 EP A2055 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27102641 ER PT J AU YERGEY, AL VIEIRA, NE ABRAMS, SA OBRIEN, KO ALDROUBI, A AF YERGEY, AL VIEIRA, NE ABRAMS, SA OBRIEN, KO ALDROUBI, A TI COMPARISON OF METHODOLOGIES FOR DETERMINATION OF TRUE FRACTIONAL ABSORPTION OF DIETARY CALCIUM SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD 20892. BEIP,BETHESDA,MD 20892. CNRC,HOUSTON,TX 77030. RI Aldroubi, Akram/J-7186-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1788 EP A1788 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101093 ER PT J AU ZENG, GC DAVE, JR GAO, L BURBELO, PD RHODES, C BERNTON, E YAMADA, Y CHIANG, PK AF ZENG, GC DAVE, JR GAO, L BURBELO, PD RHODES, C BERNTON, E YAMADA, Y CHIANG, PK TI REQUIREMENT FOR THE C-FOS PROTOONCOGENE FOR THE INDUCTION OF 3T3-L1 FIBROBLASTS TO ADIPOCYTES BY INSULIN AND 3-DEAZAADENOSINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 WALTER REED ARMY MED CTR,WASHINGTON,DC 20307. NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1936 EP A1936 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101951 ER PT J AU ZHANG, J HERMAN, EH FERRANS, VJ AF ZHANG, J HERMAN, EH FERRANS, VJ TI INTERSTITIAL DENDRITIC CELLS IN THE HEARTS OF SPONTANEOUSLY HYPERTENSIVE RATS (SHR) TREATED WITH DOXORUBICIN (DXR) AND ICRF-187 SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,LAUREL,MD. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1874 EP A1874 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101589 ER PT J AU ZHAO, F CHU, HK ASOFSKY, R AF ZHAO, F CHU, HK ASOFSKY, R TI INSITU HYBRIDIZATION STUDY OF ACTIVATION OF LYMPHOKINE GENES IN INDIVIDUAL CELLS OF A CLONED T-CELL LINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1622 EP A1622 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27100133 ER PT J AU ZWEERINK, H GAMMON, M PARKER, K BEDNAREK, M BIDDISON, W CUNNINGHAM, B HERMES, J PORTER, G SAUMA, S TAMHANKAR, S WILLIAMSON, A AF ZWEERINK, H GAMMON, M PARKER, K BEDNAREK, M BIDDISON, W CUNNINGHAM, B HERMES, J PORTER, G SAUMA, S TAMHANKAR, S WILLIAMSON, A TI ENDOGENOUS AND EXOGENOUS LOADING OF HLA-A2 BY PEPTIDE ANALOGS OF THE INFLUENZA-VIRUS MATRIX PROTEIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 MERCK SHARP & DOHME LTD,RAHWAY,NJ 07065. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 28 PY 1992 VL 6 IS 5 BP A1800 EP A1800 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HH271 UT WOS:A1992HH27101159 ER PT J AU PLOTZ, PH AF PLOTZ, PH TI NEW UNDERSTANDING OF MYOSITIS SO HOSPITAL PRACTICE LA English DT Article RP PLOTZ, PH (reprint author), NIAMSD,ARTHRITIS & RHEUMATISM BRANCH,CONNECT TISSUE DIS SECT,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MCGRAW HILL HEALTHCARE PUBLICATIONS PI MINNEAPOLIS PA 4530 WEST 77TH ST, MINNEAPOLIS, MN 55435-5000 SN 8750-2836 J9 HOSP PRACT JI Hosp. Pract. PD FEB 28 PY 1992 VL 27 IS 2A BP 33 EP 43 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA HG310 UT WOS:A1992HG31000008 PM 1310690 ER PT J AU FOLLMANN, D WITTES, J CUTLER, JA AF FOLLMANN, D WITTES, J CUTLER, JA TI THE USE OF SUBJECTIVE RANKINGS IN CLINICAL-TRIALS WITH AN APPLICATION TO CARDIOVASCULAR-DISEASE SO STATISTICS IN MEDICINE LA English DT Article ID MULTIPLE ENDPOINTS AB Evaluating a clinical trial can be problematic if the studied treatments affect patients in many ways. A possible method for evaluating treatments is to have raters rank all the patients' trial experiences and then test whether the distribution of ranks differ between treatments. Before one can advocate such a procedure, however, one would like to be assured that raters agree fairly well with one another. As a first step in. examining whether raters tend to agree, we conducted a small study with 20 raters evaluating 43 trial experiences from an imaginary cardiovascular clinical trial. Raters showed a high degree of consensus. Moreover, the average ranks agreed fairly well with two quantitative ranking rules. On the other hand, the average ranks did not agree very well with weightings usually selected in cardiovascular trials. These results suggest ranking may be a feasible approach to analysing certain clinical trials with multiple outcomes. C1 STAT COLLABORAT,WASHINGTON,DC 20008. NHLBI,BETHESDA,MD 20892. RP FOLLMANN, D (reprint author), NHLBI,7550 WISCONSIN AVE,BETHESDA,MD 20892, USA. NR 10 TC 24 Z9 24 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD FEB 28 PY 1992 VL 11 IS 4 BP 427 EP 437 DI 10.1002/sim.4780110402 PG 11 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA HL241 UT WOS:A1992HL24100001 PM 1609177 ER PT J AU FOLLMANN, D WITTES, J CUTLER, JA AF FOLLMANN, D WITTES, J CUTLER, JA TI THE USE OF SUBJECTIVE RANKINGS IN CLINICAL-TRIALS WITH AN APPLICATION TO CARDIOVASCULAR-DISEASE - REJOINDER SO STATISTICS IN MEDICINE LA English DT Editorial Material C1 STAT COLLABORAT,WASHINGTON,DC 20008. NHLBI,BETHESDA,MD 20892. RP FOLLMANN, D (reprint author), NHLBI,7550 WISCONSIN AVE,BETHESDA,MD 20892, USA. NR 2 TC 1 Z9 1 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD FEB 28 PY 1992 VL 11 IS 4 BP 453 EP 454 DI 10.1002/sim.4780110407 PG 2 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA HL241 UT WOS:A1992HL24100006 ER PT J AU FEUER, EJ HANKEY, BF GAYNOR, JJ WESLEY, MN BAKER, SG MEYER, JS AF FEUER, EJ HANKEY, BF GAYNOR, JJ WESLEY, MN BAKER, SG MEYER, JS TI GRAPHICAL REPRESENTATION OF SURVIVAL CURVES ASSOCIATED WITH A BINARY NON-REVERSIBLE TIME-DEPENDENT COVARIATE SO STATISTICS IN MEDICINE LA English DT Article ID HEART-TRANSPLANT DATA; PARTIALLY CENSORED-DATA; CANCER CLINICAL-TRIALS; SEMI-MARKOV MODELS; RESPONDER AB The use of time dependent covariates has allowed for incorporation into analysis of survival data intervening events that are binary and non-reversible (for example, heart transplant, initial response to chemotherapy). We can represent this type of intervening event as a three-state stochastic process with a starting state (S), an intervening state (I), and an absorbing state (D), which usually represents death. In this paper we present three procedures for calculating survivorship functions which attempt to display the prognostic significance of the time dependent covariate. The first method compares survival from baseline for the two possible paths through the stochastic process; the second method compares overall survival to survival with state I removed from the process; and, the third method compares survival for those already in state I at a landmark time x to those in state S at time x who will never enter state I. We develop discrete hazard estimates for the survival curves associated with the three methods. Two examples illustrate how these methods can yield different results and in which situations one might employ each of the three methods. Extensions to applications with reversible binary time dependent covariates and models with both baseline and time dependent covariates are suggested. C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. MEM SLOAN KETTERING CANC CTR,DIV BIOSTAT,NEW YORK,NY 10021. IMS INC,SILVER SPRING,MD 20910. RP FEUER, EJ (reprint author), NCI,DIV CANC PREVENT & CONTROL,EPN 313,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA-47179] NR 20 TC 10 Z9 10 U1 0 U2 1 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD FEB 28 PY 1992 VL 11 IS 4 BP 455 EP 474 DI 10.1002/sim.4780110408 PG 20 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA HL241 UT WOS:A1992HL24100007 PM 1609178 ER PT J AU NAM, JM FEARS, TR AF NAM, JM FEARS, TR TI OPTIMUM SAMPLE-SIZE DETERMINATION IN STRATIFIED CASE-CONTROL STUDIES WITH COST CONSIDERATIONS SO STATISTICS IN MEDICINE LA English DT Article ID POWER AB We investigate sample size determination for Cochran's test for stratified case-control studies when samples of cases and controls are allocated to maximize the asymptotic efficiency of Cochran's test subject to fixed total cost with cost per control varying by strata. We consider two situations typical of strata-matched case-control studies: when one samples both cases and controls and when cases are given and one samples controls. In each situation we develop and study an asymptotic method for finding the sample size required for a specific power under the optimum allocation proposed by Nam and Fears. Also, for the second situation, we investigate an asymptotic method for determining the common ratio, k, in one-to-k strata-matched case-control studies without cost consideration for a given power. When cases are given, neither the optimum nor the standard control sample sizes appear in a closed form; we present numerical methods for calculating these sample sizes and illustrate them with examples. We find the reduction in total cost obtained under the optimum allocation compared to standard allocation more pronounced as the differences in stratum-specific costs of sampling controls increase. RP NAM, JM (reprint author), NCI,BIOSTAT BRANCH,ROCKVILLE,MD 20892, USA. NR 8 TC 5 Z9 5 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD FEB 28 PY 1992 VL 11 IS 4 BP 547 EP 556 DI 10.1002/sim.4780110416 PG 10 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA HL241 UT WOS:A1992HL24100015 PM 1609184 ER PT J AU ALBORNOZ, L JONES, JM VEECH, RL AF ALBORNOZ, L JONES, JM VEECH, RL TI POTENTIAL ROLE OF ACTIVATED LYMPHOCYTES IN THE PATHOGENESIS OF ALCOHOLIC LIVER-DISEASE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,ROCKVILLE,MD 20852. UNIV ARKANSAS MED SCI HOSP,DEPT PATHOL,LITTLE ROCK,AR 72205. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1026 EP A1026 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900521 ER PT J AU BABILA, T KLEIN, DC AF BABILA, T KLEIN, DC TI NEURAL REGULATION OF THE STIMULATORY GTP-BINDING PROTEIN IN THE RAT PINEAL-GLAND SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1551 EP A1551 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903560 ER PT J AU BARNES, DM SYKES, DB SHECHTER, Y MILLER, DS AF BARNES, DM SYKES, DB SHECHTER, Y MILLER, DS TI STIMULATION OF PROTEIN-SYNTHESIS IN XENOPUS-LAEVIS OOCYTES BY VANADATE AND PEROXIDE OF VANADATE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. WEIZMANN INST SCI,IL-76100 REHOVOT,ISRAEL. NR 1 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1073 EP A1073 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900792 ER PT J AU BEVERLY, B KANG, SM BRORSON, KA TRAN, A LENARDO, MJ SCHWARTZ, RH AF BEVERLY, B KANG, SM BRORSON, KA TRAN, A LENARDO, MJ SCHWARTZ, RH TI MOLECULAR MECHANISM OF THE INTERLEUKIN-2(IL-2) PRODUCTION DEFECT IN T-CELL ANERGY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1408 EP A1408 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902734 ER PT J AU BIANCHINE, P BURD, P METCALFE, D AF BIANCHINE, P BURD, P METCALFE, D TI UNSTIMULATED MAST-CELLS PROLIFERATE IN RESPONSE TO SIGNALS TRANSDUCED VIA CELL-SURFACE VITRONECTIN RECEPTORS AND PLATE-BOUND BUT NOT SOLUBLE VITRONECTIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1403 EP A1403 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902706 ER PT J AU CEMANBOGNER, S RUDERSDORF, R LONG, E DEMARS, R AF CEMANBOGNER, S RUDERSDORF, R LONG, E DEMARS, R TI A GENE IN THE CLASS-II REGION OF THE MHC IS NEEDED FOR NORMAL CONFORMATION AND ANTIGEN PRESENTATION BY HLA DR MOLECULES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV WISCONSIN,MADISON,WI 53706. NIH,ROCKVILLE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1126 EP A1126 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901096 ER PT J AU CHEN, HT MAGE, RG AF CHEN, HT MAGE, RG TI VH GENE-EXPRESSION AND REGULATION IN THE MUTANT ALICIA RABBIT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1221 EP A1221 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901647 ER PT J AU DAI, YS BAUM, BJ AF DAI, YS BAUM, BJ TI ALPHA-1A-ADRENERGIC RECEPTORS COUPLE TO INTRACELLULAR CA2+ MOBILIZATION IN RAT PAROTID ACINAR-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,CIPCB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1021 EP A1021 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900496 ER PT J AU DELESPESSE, G FARGEAS, C WU, CY COX, D NUTMAN, T AF DELESPESSE, G FARGEAS, C WU, CY COX, D NUTMAN, T TI DIFFERENTIAL EFFECT OF TGF-BETA ON THE SYNTHESIS OF TH1 AND TH2 TYPES OF LYMPHOKINES BY HUMAN-T LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MONTREAL,MONTREAL H3C 3J7,QUEBEC,CANADA. CIBA GEIGY AG,CH-4002 BASEL,SWITZERLAND. NIH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1417 EP A1417 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902783 ER PT J AU DONOHUE, SJ MCBRIDE, OW ROSEBOOM, PH ILLNEROVA, H WELLER, JL KLEIN, DC AF DONOHUE, SJ MCBRIDE, OW ROSEBOOM, PH ILLNEROVA, H WELLER, JL KLEIN, DC TI HUMAN HYDROXYINDOLE-O-METHYLTRANSFERASE (HIOMT) - HETEROGENEITY AND CHROMOSOMAL LOCALIZATION TO PSEUDOAUTOSOMAL XY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. NCI,BETHESDA,MD 20892. INST PHYSIOL,PRAGUE,CZECHOSLOVAKIA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1275 EP A1275 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901956 ER PT J AU ELGAMIL, M WINKLER, D GRANGER, L SHARROW, S WUNDERLICH, J AF ELGAMIL, M WINKLER, D GRANGER, L SHARROW, S WUNDERLICH, J TI LIPOPOLYSACCHARIDE REACTS WITH LYMPHOCYTES-B TO ACTIVATE MOUSE NATURAL-KILLER-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1338 EP A1338 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902326 ER PT J AU FLESSNER, MF MEJIA, R KNEPPER, MA AF FLESSNER, MF MEJIA, R KNEPPER, MA TI THE ASCENDING THIN LIMB ACTS AS AN EQUILIBRATING SEGMENT FOR AMMONIUM AND BICARBONATE TRANSPORT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A958 EP A958 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900128 ER PT J AU FONTVIEILLE, AM RISING, R FERRARO, B LARSON, E RAVUSSIN, E AF FONTVIEILLE, AM RISING, R FERRARO, B LARSON, E RAVUSSIN, E TI SLEEPING METABOLIC-RATE VS SLEEP STAGES IN MAN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1083 EP A1083 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900854 ER PT J AU FUSCHIOTTI, P HARINDRANATH, N MAGE, RG DHANARAJAN, P ROUX, KH AF FUSCHIOTTI, P HARINDRANATH, N MAGE, RG DHANARAJAN, P ROUX, KH TI THE RECOMBINASE ACTIVATING GENES RAG-1 AND RAG-2 OF THE RABBIT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. NIDR,ORAL MED LAB,BETHESDA,MD 20892. FLORIDA STATE UNIV,DEPT BIOL SCI,TALLAHASSEE,FL 32306. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1223 EP A1223 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901657 ER PT J AU GORDON, JR KENDALL, JC FRANKEL, SK BURD, PR GALLI, SJ AF GORDON, JR KENDALL, JC FRANKEL, SK BURD, PR GALLI, SJ TI MAST CELL-DEPENDENT INDUCTION OF MESSENGER-RNA FOR JE, THE MURINE HOMOLOG OF HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN, INVIVO DURING PASSIVE CUTANEOUS ANAPHYLAXIS (PCA) RESPONSES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV SASKATCHEWAN,DEPT VET MICROBIOL,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA. BETH ISRAEL HOSP,DEPT PATHOL,BOSTON,MA 02215. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. NIH,BETHESDA,MD 20892. NR 1 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1147 EP A1147 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901222 ER PT J AU GOURLEY, MF KISCH, WJ KING, LB STEINBERG, AD AF GOURLEY, MF KISCH, WJ KING, LB STEINBERG, AD TI ANALYSIS OF A TYPE-C POLYTROPIC MURINE RETROVIRUS FROM AN NZB MOUSE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1047 EP A1047 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900642 ER PT J AU GUNDIMEDA, U HARA, SK ANDERSON, WB GOPALAKRISHNA, R AF GUNDIMEDA, U HARA, SK ANDERSON, WB GOPALAKRISHNA, R TI RETINOIDS PROTECT PROTEIN-KINASE-C FROM OXIDATIVE MODIFICATION INDUCED BY OXIDANT TUMOR PROMOTERS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV SO CALIF,SCH MED,DEPT PHARMACOL & NUTR,LOS ANGELES,CA 90033. NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1596 EP A1596 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903816 ER PT J AU HAN, JS THOMPSON, KA CHOU, CL KNEPPER, MA AF HAN, JS THOMPSON, KA CHOU, CL KNEPPER, MA TI EXPERIMENTAL TESTS OF 3-DIMENSIONAL MODEL OF URINARY CONCENTRATING MECHANISM SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A957 EP A957 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900120 ER PT J AU HERMAN, E CHADWICK, D ZHANG, J FERRANS, V AF HERMAN, E CHADWICK, D ZHANG, J FERRANS, V TI MINOXIDIL-INDUCED CARDIAC LESIONS IN RATS OF DIFFERENT AGES SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,LAUREL,MD. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1306 EP A1306 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902138 ER PT J AU HIRAIWA, M OBRIEN, JS KISHIMOTO, Y MARTIN, BM GINNS, EL FLUHARTY, AL AF HIRAIWA, M OBRIEN, JS KISHIMOTO, Y MARTIN, BM GINNS, EL FLUHARTY, AL TI ISOLATION AND CHARACTERIZATION OF PROSAPOSIN, THE PRECURSOR OF SAPOSINS (SPHINGOLIPID ACTIVATOR PROTEINS) SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF SAN DIEGO,LA JOLLA,CA 92093. NIMH,NEUROGENET SECT,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,LANTERNMAN DEV CTR,MRRC RES GRP,LOS ANGELES,CA 90024. NR 0 TC 3 Z9 3 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A969 EP A969 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900187 ER PT J AU HURSTING, S SWITZER, B FRENCH, J KARI, F AF HURSTING, S SWITZER, B FRENCH, J KARI, F TI GROWTH-HORMONE MEDIATES THE DIET RESTRICTION-INDUCED INHIBITION OF MONONUCLEAR CELL LEUKEMIA IN FISCHER RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV N CAROLINA,DEPT NUTR,CHAPEL HILL,NC 27599. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1397 EP A1397 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902670 ER PT J AU JAFFE, JS STROBER, W SNELLER, MC AF JAFFE, JS STROBER, W SNELLER, MC TI FUNCTIONAL ABNORMALITIES OF CD8+ T-CELLS DEFINE A UNIQUE SUBSET OF PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,MUCOSAL IMMUN SECT,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1120 EP A1120 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901067 ER PT J AU JAGADEESH, G ISHAC, EJN KUNOS, G AF JAGADEESH, G ISHAC, EJN KUNOS, G TI PROTEIN-KINASE-C (PKC)-INDUCED MODULATION OF ALPHA-1-ADRENERGIC RECEPTORS (ALPHA-1AR) IN RAT HEPATOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,CDER,ROCKVILLE,MD 20857. NIAAA,LMCN,BETHESDA,MD 20892. RI Jagadeesh, Gowraganahalli/G-6408-2010 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1562 EP A1562 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903620 ER PT J AU JETT, M FINE, RL GEMSKI, P BOYLE, T HUNT, R CHATTERJEE, S MULSHINE, JL AF JETT, M FINE, RL GEMSKI, P BOYLE, T HUNT, R CHATTERJEE, S MULSHINE, JL TI LEUKOTRIENES VS 5-HETE - STAPHYLOCOCCAL ENTEROTOXIN-B (SEB) INDUCED 5-LIPOXYGENASE METABOLITES REFLECT TOXIC VS NONTOXIC RESPONSES SO FASEB JOURNAL LA English DT Meeting Abstract C1 WALTER REED ARMY MED CTR,DIV PATHOL,WASHINGTON,DC 20307. DUKE UNIV,MED CTR,DURHAM,NC 27710. JOHNS HOPKINS UNIV,DEPT PEDIAT,BALTIMORE,MD 21218. NIH,DCPC,BPRB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1053 EP A1053 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900678 ER PT J AU KATYAL, SL OGASAWARA, H MASUHARA, M NAKAMURA, T SINGH, G HOOK, G SHINOZUKA, H AF KATYAL, SL OGASAWARA, H MASUHARA, M NAKAMURA, T SINGH, G HOOK, G SHINOZUKA, H TI EXPRESSION OF HEPATOCYTE GROWTH-FACTOR AND OF ITS RECEPTOR IN RAT LUNG SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV PITTSBURGH,SCH MED,DEPT PATHOL,PITTSBURGH,PA 15261. KYUSHU UNIV,FUKUOKA,JAPAN. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1162 EP A1162 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901306 ER PT J AU KATZ, P WHALEN, G KEHRL, JH AF KATZ, P WHALEN, G KEHRL, JH TI IDENTIFICATION OF A NOVEL PROTEIN-KINASE DIFFERENTIALLY EXPRESSED IN GERMINAL CENTER VERSUS NON-GERMINAL CENTER LYMPHOCYTES-B SO FASEB JOURNAL LA English DT Meeting Abstract C1 GEORGETOWN UNIV,WASHINGTON,DC 20007. NIAID,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1127 EP A1127 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901104 ER PT J AU KERSTING, U SPRING, KR AF KERSTING, U SPRING, KR TI KETOCONAZOLE ACTIVATES CL- CONDUCTANCE AND REVERSES FLUID TRANSPORT IN A CULTURED CYSTIC-FIBROSIS CELL-LINE (CFPAC-1) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,KIDNEY & ELECTROLYTE METAB LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1238 EP A1238 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901741 ER PT J AU KORTY, PE TEDDER, TF SHEVACH, EM AF KORTY, PE TEDDER, TF SHEVACH, EM TI IDENTIFICATION OF THE LIGANDS FOR THE CD59 AND LY-6A.2 CELL-SURFACE ANTIGENS SO FASEB JOURNAL LA English DT Meeting Abstract C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115. NIAID,LI,BETHESDA,MD 20892. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1224 EP A1224 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901664 ER PT J AU LAMORTE, VJ HAROOTUNIAN, AT SPIEGEL, AM TSIEN, RY FERAMISCO, JR AF LAMORTE, VJ HAROOTUNIAN, AT SPIEGEL, AM TSIEN, RY FERAMISCO, JR TI BIOLOGICAL-ACTIVITIES OF BRADYKININ ARE REGULATED BY THE GQ FAMILY SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,HOWARD HUGHES INST,LA JOLLA,CA 92093. NIDDK,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1355 EP A1355 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902427 ER PT J AU LARSON, DE RISING, R FERRARO, R RAVUSSIN, E AF LARSON, DE RISING, R FERRARO, R RAVUSSIN, E TI A CAFETERIA DIET IN MAN - RESULTS OF SPONTANEOUS OVERFEEDING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CLIN DIABET & NUTR SECT,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1211 EP A1211 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901588 ER PT J AU LITTLE, J ASOFSKY, R AF LITTLE, J ASOFSKY, R TI ADAPTIVE HETEROGENEITY IN RESPONSES OF INDIVIDUAL CELLS FROM A CLONED LYMPHOCYTE-B LINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1413 EP A1413 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902761 ER PT J AU LUCE, GEG SCHWEIGHOFFER, T TANAKA, Y HORGAN, KJ LAZAROVITS, AI PALS, S BUCK, D SHAW, S AF LUCE, GEG SCHWEIGHOFFER, T TANAKA, Y HORGAN, KJ LAZAROVITS, AI PALS, S BUCK, D SHAW, S TI VLA-ALPHA-4 (CD49D) AND VLA-BETA-1 (CD29) IDENTIFY AT LEAST 3-SUBSETS OF HUMAN CD4-POSITIVE MEMORY (CD45RO+) T-CELLS - SYSTEMATIC PHENOTYPIC ANALYSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EIB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1415 EP A1415 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902773 ER PT J AU MALI, V CULVER, KW CHANG, AE SHU, SY BLAESE, RM FOX, BA AF MALI, V CULVER, KW CHANG, AE SHU, SY BLAESE, RM FOX, BA TI DEVELOPMENT OF LIMITING DILUTION ANALYSIS AS A METHOD FOR QUANTITATIVE-ANALYSIS OF TUMOR INFILTRATING LYMPHOCYTE TRAFFICKING AND SURVIVAL INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MICHIGAN,DEPT SURG,ANN ARBOR,MI 48109. NCI,METAB BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1434 EP A1434 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902886 ER PT J AU MANGELS, AR BLOCK, G FREY, CM MORRIS, VC LEVANDER, OA TAYLOR, PR PATTERSON, BH NORKUS, E AF MANGELS, AR BLOCK, G FREY, CM MORRIS, VC LEVANDER, OA TAYLOR, PR PATTERSON, BH NORKUS, E TI ASCORBIC-ACID (AA) RELATIVE BIOAVAILABILITY IN HUMANS ASSESSED BY PLASMA RESPONSE TO INGESTION OF AA AS FRUIT, VEGETABLES OR IN SYNTHETIC FORM SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS,BELTSVILLE AGR RES CTR,HUMAN NUTR RES CTR,BELTSVILLE,MD 20705. NCI,BETHESDA,MD 20892. OUR LADY MERCY MED CTR,BRONX,NY 10466. RI Block, Gladys/E-3304-2010 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1376 EP A1376 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902543 ER PT J AU MANI, C GELBOIN, HV PARK, SS KUPFER, D AF MANI, C GELBOIN, HV PARK, SS KUPFER, D TI CYTOCHROME-P-450 AND FLAVIN-CONTAINING MONOOXYGENASE (FMO) METABOLISM OF THE ANTICANCER AGENT TAMOXIFEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1569 EP A1569 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903664 ER PT J AU MASON, AT MCVICAR, DW SMITH, CA WARE, C ORTALDO, JR AF MASON, AT MCVICAR, DW SMITH, CA WARE, C ORTALDO, JR TI REGULATION OF IL-2 ACTIVATION OF NON-MHC RESTRICTED LYSIS - ROLE OF TNF-ALPHA RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES FACIL,EXPTL IMMUNOL LAB,FREDERICK,MD 21701. UNIV CALIF RIVERSIDE,RIVERSIDE,CA 92521. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1424 EP A1424 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902825 ER PT J AU MCGIMSEY, WC IADAROLA, MJ STUMPF, WE SAR, M AF MCGIMSEY, WC IADAROLA, MJ STUMPF, WE SAR, M TI CYTOPLASMIC LOCALIZATION OF GLUCOCORTICOID RECEPTOR NOT CORRELATED WITH FRA INDUCTION IN RAT PARAVENTRICULAR NUCLEUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV N CAROLINA,DEPT ANAT,CHAPEL HILL,NC 27599. NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1288 EP A1288 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902031 ER PT J AU MONSMA, FJ SHEN, Y MAHAN, LC SIBLEY, DR AF MONSMA, FJ SHEN, Y MAHAN, LC SIBLEY, DR TI IDENTIFICATION OF A NOVEL G-PROTEIN-COUPLED RECEPTOR WHICH EXHIBITS HIGH HOMOLOGY TO CLONED DOPAMINE-RECEPTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. NIMH,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1556 EP A1556 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903589 ER PT J AU MUEGGE, K VILA, M MUSSO, T HERRLICH, P STEIN, B DURUM, SK AF MUEGGE, K VILA, M MUSSO, T HERRLICH, P STEIN, B DURUM, SK TI IL-1-RESPONSIVE ELEMENTS ON THE C-JUN PROMOTER SO FASEB JOURNAL LA English DT Meeting Abstract C1 PRI DYNCORP,BCDP,FREDERICK,MD. NCI,FREDERICK CANC RES DEV CTR,BETHESDA,MD 20892. KERNFORSCHUNGSZENTRUM KARLSRUHE GMBH,W-7500 KARLSRUHE 1,GERMANY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1122 EP A1122 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901076 ER PT J AU MUTHUSAMY, N PARK, DJ RHO, HW RHEE, SG SUBBARAO, B AF MUTHUSAMY, N PARK, DJ RHO, HW RHEE, SG SUBBARAO, B TI DIFFERENTIAL EXPRESSION OF PHOSPHOLIPASE-C ISOZYMES IN MURINE LYMPHOCYTES-B SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV KENTUCKY,DEPT MICROBIOL IMMUNOL,LEXINGTON,KY 40506. UNIV KENTUCKY,SANDERS BROWN CTR AGING,LEXINGTON,KY 40506. NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RI Muthusamy, Natarajan/A-6915-2009 NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1128 EP A1128 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901107 ER PT J AU NIELSEN, S KNEPPER, MA AF NIELSEN, S KNEPPER, MA TI INDEPENDENT PHYSICAL PROCESSES ALTER UREA AND WATER PERMEABILITY OF RAT TERMINAL COLLECTING DUCT IN RESPONSE TO VASOPRESSIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A957 EP A957 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900121 ER PT J AU OPARA, E GARFINKEL, M HUBBARD, V AKWARI, O AF OPARA, E GARFINKEL, M HUBBARD, V AKWARI, O TI EFFECT OF FATTY-ACIDS ON INSULIN RELEASE - ROLE OF CHAIN-LENGTH AND DEGREE OF UNSATURATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 DUKE UNIV,MED CTR,DURHAM,NC 27710. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1495 EP A1495 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903240 ER PT J AU PANAYI, GS CHIKANZA, IC PETROU, P KINGSLEY, G CHROUSOS, G AF PANAYI, GS CHIKANZA, IC PETROU, P KINGSLEY, G CHROUSOS, G TI DEFECTIVE HYPOTHALAMIC PITUITARY-ADRENAL AXIS FUNCTION IN RHEUMATOID-ARTHRITIS IS NOT A CONSEQUENCE OF CHRONIC INFLAMMATION PER SE SO FASEB JOURNAL LA English DT Meeting Abstract C1 FIZIOTERAPIAS INT,ORSZAGOS REUMA,BUDAPEST,HUNGARY. NIH,BETHESDA,MD 20892. UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,GUYS HOSP,RHEUMATOL UNIT,LONDON SE1 9RT,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1448 EP A1448 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902966 ER PT J AU PARK, KS SOHN, DH KIM, BM VEECH, RL SONG, BJ AF PARK, KS SOHN, DH KIM, BM VEECH, RL SONG, BJ TI SPECIFIC TRANSLATIONAL INDUCTION OF CYTOCHROME-P450IIE BY ISONIAZID SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,METAB & MOLEC BIOL LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1274 EP A1274 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901950 ER PT J AU PATTEN, C AOYAMA, T LEE, MJ NING, SM GONZALEZ, F YANG, CS AF PATTEN, C AOYAMA, T LEE, MJ NING, SM GONZALEZ, F YANG, CS TI CATALYTIC ACTIVITY OF HUMAN P-450 2E1 PRODUCED BY THE VACCINIA VIRUS EXPRESSION SYSTEM SO FASEB JOURNAL LA English DT Meeting Abstract C1 RUTGERS STATE UNIV,COLL PHARM,CANC RES LAB,PISCATAWAY,NJ 08854. NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1202 EP A1202 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901533 ER PT J AU PELLEGRINO, SM FRASER, CM AF PELLEGRINO, SM FRASER, CM TI DIFFERENTIAL AGONIST BINDING-PROPERTIES OF BETA-2-ADRENERGIC AND BETA-3-ADRENERGIC RECEPTORS EXPRESSED IN CHO CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,NEUROGENET LAB,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1562 EP A1562 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903623 ER PT J AU RISING, R FONTVIEILLE, AM LARSON, DE FERRARO, R ANDERSON, T RAVUSSIN, E AF RISING, R FONTVIEILLE, AM LARSON, DE FERRARO, R ANDERSON, T RAVUSSIN, E TI DIFFERENCES IN DAY NIGHT BODY CORE TEMPERATURE BETWEEN CAUCASIAN AND PIMA INDIAN MEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1083 EP A1083 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900852 ER PT J AU ROSADA, C DELLABELLA, D KRISHNA, G AF ROSADA, C DELLABELLA, D KRISHNA, G TI INVITRO METHOD FOR SCREENING PHYSICAL-DEPENDENCE AND TOLERANCE OF MORPHINE ANALOGS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. UNIV MILAN,DEPT PHARM,I-20122 MILAN,ITALY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1574 EP A1574 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903692 ER PT J AU ROSEBOOM, PH WELLER, JL NAMBOODIRI, MAA TOKER, A AITKEN, A KLEIN, DC AF ROSEBOOM, PH WELLER, JL NAMBOODIRI, MAA TOKER, A AITKEN, A KLEIN, DC TI 14-3-3 PROTEINS - ISOLATION, CLONING AND DISTRIBUTION OF THE EPSILON-ISOFORM SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD 20892. GEORGETOWN UNIV,DEPT BIOL,WASHINGTON,DC 20057. NATL INST MED RES,LONDON NW7 1AA,ENGLAND. NR 0 TC 7 Z9 7 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1516 EP A1516 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903356 ER PT J AU SATO, K DOBSON, G RAWLINGS, R VEECH, RL AF SATO, K DOBSON, G RAWLINGS, R VEECH, RL TI THE EFFECTS OF ALTERATION OF PERFUSATE FREE [MG2+] ON CALCIUM CONTENT AND PHYSIOLOGICAL PERFORMANCE IN WORKING RAT-HEART SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,METAB & MOLEC BIOL LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1268 EP A1268 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901918 ER PT J AU SCOTT, DE KISCH, WJ STEINBERG, AD AF SCOTT, DE KISCH, WJ STEINBERG, AD TI CLONAL ANERGY AND DELETION OF T-CELLS IN LUPUS-PRONE MICE AFTER INVIVO TREATMENT WITH STAPHYLOCOCCAL ENTEROTOXIN-B SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1024 EP A1024 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900511 ER PT J AU SCULLY, G COHEN, DI MCCOY, S LANE, HC AF SCULLY, G COHEN, DI MCCOY, S LANE, HC TI THE ROLE OF VPU IN THE PATHOGENESIS OF THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,LIR,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1328 EP A1328 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902266 ER PT J AU SEDER, RA PLAUT, M DVORAK, A GALLI, S PAUL, WE AF SEDER, RA PLAUT, M DVORAK, A GALLI, S PAUL, WE TI FC-EPSILON-RI+, C-KIT- CELLS WITH THE CHARACTERISTICS OF BASOPHILS PRODUCE IL-4 IN RESPONSE TO FCR CROSS-LINKAGE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. HARVARD UNIV,SCH MED,BOSTON,MA 02115. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1402 EP A1402 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902701 ER PT J AU SICA, A TAN, TH RICE, N KRETZSCHMAR, M GHOSH, P YOUNG, HA AF SICA, A TAN, TH RICE, N KRETZSCHMAR, M GHOSH, P YOUNG, HA TI IDENTIFICATION OF NOVEL PROTEIN-BINDING SEQUENCES IN THE 1ST INTRON OF HUMAN INTERFERON-GAMMA-GENOMIC DNA SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES DEV CTR,EXPTL IMMUNOL LAB,BETHESDA,MD 20892. NCI,FREDERICK CANC RES DEV CTR,PRI DYNCORP,BCDP,BETHESDA,MD 20892. NCI,FREDERICK CANC RES DEV CTR,APPL BIOSCI LAB,BETHESDA,MD 20892. ROCKEFELLER UNIV,NEW YORK,NY 10021. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1122 EP A1122 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901077 ER PT J AU SLAUTER, RW RAYMER, JH VELEZ, GR GAUDETTE, NF BUCHER, JR AF SLAUTER, RW RAYMER, JH VELEZ, GR GAUDETTE, NF BUCHER, JR TI THE COLLECTION AND ANALYSIS OF ENDOGENOUS ORGANIC-CONSTITUENTS FOUND IN EXPIRED RAT BREATH SO FASEB JOURNAL LA English DT Meeting Abstract C1 RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1583 EP A1583 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903741 ER PT J AU SONG, L ADLER, W KIM, Y COLLINS, G NAGEL, J AF SONG, L ADLER, W KIM, Y COLLINS, G NAGEL, J TI CD2 BUT NOT CD3-TI MIMICS PMA IN INDUCING RAPID TYROSINE PHOSPHORYLATION OF A 41-KDA PROTEIN IN THE HUMAN JURKAT T-CELL LINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,GERONTOL RES CTR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1225 EP A1225 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901665 ER PT J AU STROMBERG, C NAVERI, L SAAVEDRA, JM AF STROMBERG, C NAVERI, L SAAVEDRA, JM TI REGULATION OF RAT CEREBRAL BLOOD-FLOW BY ANGIOTENSIN-II SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CLIN SCI LAB,PHARMACOL SECT,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1011 EP A1011 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900435 ER PT J AU SYKES, M BODINE, DM MOULTON, AD PEARSON, DA NIENHUIS, AW SACHS, DH AF SYKES, M BODINE, DM MOULTON, AD PEARSON, DA NIENHUIS, AW SACHS, DH TI TOLERANCE INDUCTION USING AUTOLOGOUS BONE-MARROW MODIFIED WITH AN ALLOGENEIC CLASS-I MHC GENE SO FASEB JOURNAL LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA 02114. NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1405 EP A1405 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902719 ER PT J AU TALAN, M ENGEL, B AF TALAN, M ENGEL, B TI IN DIURNAL PRIMATES BLOOD-VISCOSITY IS HIGHER IN THE MORNING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1529 EP A1529 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903431 ER PT J AU TANAKA, Y ALBELDA, SM HORGAN, KJ VANSEVENTER, GA SHIMIZU, Y LUCE, GEG HALLAM, J NEWMAN, W NEWMAN, PJ BUCK, CA SHAW, S AF TANAKA, Y ALBELDA, SM HORGAN, KJ VANSEVENTER, GA SHIMIZU, Y LUCE, GEG HALLAM, J NEWMAN, W NEWMAN, PJ BUCK, CA SHAW, S TI CD31/PECAM-1 IS AN ADHESION AMPLIFIER ON UNIQUE T-CELL SUBSETS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,EIB,BALTIMORE,MD 21211. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1125 EP A1125 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901091 ER PT J AU TEAGUE, WE DOBSON, GP AF TEAGUE, WE DOBSON, GP TI EFFECT OF TEMPERATURE ON THE CREATINE-KINASE REACTION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,METAB LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1267 EP A1267 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901909 ER PT J AU TELLA, SR KORUPOLU, GR SCHINDLER, CW GOLDBERG, SR AF TELLA, SR KORUPOLU, GR SCHINDLER, CW GOLDBERG, SR TI PATHOPHYSIOLOGICAL MECHANISMS OF LETHALITY PRODUCED BY ACUTE OVERDOSE OF COCAINE IN RODENTS UNDER VARIOUS EXPERIMENTAL CONDITIONS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. GEORGETOWN UNIV,SCH MED,WASHINGTON,DC 20007. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A987 EP A987 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900293 ER PT J AU TOLLERUD, D FUCHS, D BROWN, L MALONEY, E BLATTNER, W AF TOLLERUD, D FUCHS, D BROWN, L MALONEY, E BLATTNER, W TI INFLUENCE OF RACE AND AGE ON SERUM LEVELS OF BETA-2 MICROGLOBULIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV PITTSBURGH,PITTSBURGH,PA 15260. UNIV INNSBRUCK,A-6020 INNSBRUCK,AUSTRIA. NCI,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1326 EP A1326 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902255 ER PT J AU TSANG, KY CALVO, B OI, CF NIERODA, C DEFILIPPI, R GREINER, J KASHMIRI, S SCHLOM, J AF TSANG, KY CALVO, B OI, CF NIERODA, C DEFILIPPI, R GREINER, J KASHMIRI, S SCHLOM, J TI TRANSFER OF IL-6 GENE INTO TUMOR-CELLS AS A POSSIBLE MODALITY FOR CANCER-THERAPY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1432 EP A1432 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902872 ER PT J AU TSUTSUMI, K CHIUEH, CC SAAVEDRA, JM AF TSUTSUMI, K CHIUEH, CC SAAVEDRA, JM TI HETEROGENEITY OF AT2 ANGIOTENSIN-II RECEPTORS IN THE IMMATURE RAT-BRAIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1577 EP A1577 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903708 ER PT J AU TULP, OL BLACK, DL HANSEN, CT MICHAELIS, OE AF TULP, OL BLACK, DL HANSEN, CT MICHAELIS, OE TI THERMOGENESIS AND ADIPOSITY IN THE WKY/N-CP RAT - A NEW MODEL OF OBESITY+NIDDM SO FASEB JOURNAL LA English DT Meeting Abstract C1 DREXEL UNIV,PHILADELPHIA,PA 19104. NCI,BETHESDA,MD 20892. USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE HUMAN NUTR RES CTR,BELTSVILLE,MD 20705. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1085 EP A1085 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900864 ER PT J AU TUOMINEN, RK MCMILLIAN, MK HUDSON, PM YE, H STACHOWIAK, MK HONG, JS AF TUOMINEN, RK MCMILLIAN, MK HUDSON, PM YE, H STACHOWIAK, MK HONG, JS TI NICOTINE ACTIVATES PROTEIN-KINASE-C IN BOVINE MEDULLARY CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1561 EP A1561 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903615 ER PT J AU VEECH, RL KING, T CRUTCHFIELD, C GITOMER, W AF VEECH, RL KING, T CRUTCHFIELD, C GITOMER, W TI ETHANOL OR ACETATE ALTER THE FREE [MG2+], DELTA-G[ION]O/I, AND EN, IN OPPOSITE DIRECTIONS IN RAT-LIVER INVIVO BY OPENING DIFFERENT ION CHANNELS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,LMMB,ROCKVILLE,MD 20852. NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1056 EP A1056 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900694 ER PT J AU VISWANATHAN, M SAAVEDRA, JM AF VISWANATHAN, M SAAVEDRA, JM TI ENHANCED EXPRESSION OF ANGIOTENSIN-II AT2 RECEPTORS IN THE SKIN OF RATS DURING EXPERIMENTAL WOUND-HEALING SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,PHARMACOL SECT,LCS,BETHESDA,MD 20892. NR 0 TC 6 Z9 6 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1013 EP A1013 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900446 ER PT J AU WAKATSUKI, Y STROBER, W AF WAKATSUKI, Y STROBER, W TI THE ROLE OF GERMLINE TRANSCRIPTS IN IMMUNOGLOBULIN CLASS SWITCHING IN A LYMPHOMA CELL-LINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1134 EP A1134 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901142 ER PT J AU WILLIAMS, WM RAPOPORT, SI AF WILLIAMS, WM RAPOPORT, SI TI ISOLATION OF CEREBRAL MICROVESSELS FROM MICROWAVE-FIXED RAT-BRAIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A967 EP A967 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900176 ER PT J AU WILSON, GL KEHRL, JH AF WILSON, GL KEHRL, JH TI ANALYSIS OF THE HUMAN CD22 GENE - MAPPING THE INTRON EXON STRUCTURE AND PRELIMINARY-ANALYSIS OF THE PROMOTER REGION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1127 EP A1127 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901106 ER PT J AU WOLFF, NA PHILPOT, RM MILLER, DS PRITCHARD, JB AF WOLFF, NA PHILPOT, RM MILLER, DS PRITCHARD, JB TI FUNCTIONAL EXPRESSION OF RENAL ORGANIC ANION TRANSPORT IN XENOPUS-LAEVIS OOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1550 EP A1550 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71903552 ER PT J AU WU, JC MERLINO, GT FAUSTO, N AF WU, JC MERLINO, GT FAUSTO, N TI PROLIFERATION OF TRANSFORMING GROWTH FACTOR-ALPHA (TGF-ALPHA) TRANSGENIC MOUSE HEPATOCYTES IN PRIMARY CULTURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 BROWN UNIV,DEPT PATHOL,PROVIDENCE,RI 02912. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1028 EP A1028 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900535 ER PT J AU YEH, SY AF YEH, SY TI COMPARATIVE STIMULATORY AND ANORECTIC EFFECTS OF AMPHETAMINE (A) AND 4-HYDROXYAMPHETAMINE (4-HOA) IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1099 EP A1099 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900942 ER PT J AU YOSHIMURA, T AF YOSHIMURA, T TI NEUTROPHIL ATTRACTANT PROTEIN-1 (NAP-1) IS HIGHLY CONSERVED IN GUINEA-PIG, BUT NOT IN MOUSE OR RAT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1340 EP A1340 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71902334 ER PT J AU ZHANG, YH KRAMER, TR SHI, W WANG, ZG HUI, YQ SHI, R BROWN, CC TAYLOR, PR AF ZHANG, YH KRAMER, TR SHI, W WANG, ZG HUI, YQ SHI, R BROWN, CC TAYLOR, PR TI EFFECTS OF MICRONUTRIENT SUPPLEMENTATION ON LYMPHOCYTE-T BLASTOGENESIS OF ADULTS IN LINXIAN COUNTY, CHINA SO FASEB JOURNAL LA English DT Meeting Abstract C1 CHINESE ACAD MED SCI,INST CANC,BEIJING 100021,PEOPLES R CHINA. USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE HUMAN NUTR CTR,BELTSVILLE,MD 20705. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1103 EP A1103 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71900969 ER PT J AU ZHOU, D WINCHURCH, R CHREST, FJ AF ZHOU, D WINCHURCH, R CHREST, FJ TI IMMUNE AGING - THE ROLE OF TGF-BETA SO FASEB JOURNAL LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21224. NIH,GERONTOL RES CTR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 26 PY 1992 VL 6 IS 4 BP A1148 EP A1148 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HG719 UT WOS:A1992HG71901223 ER PT J AU RABKIN, CS HILGARTNER, MW HEDBERG, KW ALEDORT, LM HATZAKIS, A EICHINGER, S EYSTER, ME WHITE, GC KESSLER, CM LEDERMAN, MM DEMOERLOOSE, P BRAY, GL COHEN, AR ANDES, WA MANCOJOHNSON, M SCHRAMM, W KRONER, BL BLATTNER, WA GOEDERT, JJ AF RABKIN, CS HILGARTNER, MW HEDBERG, KW ALEDORT, LM HATZAKIS, A EICHINGER, S EYSTER, ME WHITE, GC KESSLER, CM LEDERMAN, MM DEMOERLOOSE, P BRAY, GL COHEN, AR ANDES, WA MANCOJOHNSON, M SCHRAMM, W KRONER, BL BLATTNER, WA GOEDERT, JJ TI INCIDENCE OF LYMPHOMAS AND OTHER CANCERS IN HIV-INFECTED AND HIV-UNINFECTED PATIENTS WITH HEMOPHILIA SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; RENAL-TRANSPLANT RECIPIENTS; NON-HODGKIN LYMPHOMA; KAPOSIS-SARCOMA; DISEASE; AIDS; RISK; PAPILLOMAVIRUS AB Objective. - To determine the types and rates of cancers occurring in excess in the presence of infection with the human immunodeficiency virus type 1 (HIV-1). Design. - Cohort analytic study of HIV-infected and HIV-uninfected subjects followed for up to 12 years. Setting. - Fifteen hemophilia treatment centers. Patients. - A total of 1701 patients with hemophilia, of whom 1065 (63%) were HIV-1 seropositive. Main Outcome Measures. -Morphologic classification and incidence rates of cancers. Main Results. - The incidence of non-Hodgkin's lymphoma after HIV seroconversion averaged 0.15 case per 100 person-years (95% confidence interval [Cl], 0.08 to 0.25) and rose exponentially with increasing duration of HIV infection. Although the greatest absolute risk of lymphoma was in the oldest age group, the relative increase compared with general population rates was 38-fold in subjects 10 to 39 years old and 12-fold in older subjects (P <.05). The CD4+ T-lymphocyte levels for lymphoma cases were similar to HIV-positive subjects without the acquired immunodeficiency syndrome (AIDS) who had been infected for the same length of time. The incidence of Kaposi's sacroma was increased 200-fold (95% Cl, 20 to 700). The incidence of cancers other than non-Hodgkin's lymphoma and Kaposi's sarcoma were not increased in the HIV-positive subjects (ratio of observed to expected cases, 0.9 [95% Cl, 0.4 to 1.9]). The HIV-negative subjects had no significant increase in cancer incidence. Conclusions. - HIV infection has restricted effects on cancer incidence that are only partly explained by immunosuppression. Paradoxically, improvements in therapy of HIV infection that prolong survival may lead to further increases in HIV-associated lymphoma. C1 NCI,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892. CORNELL UNIV,MED CTR,DEPT PEDIAT HEMATOL ONCOL,NEW YORK,NY 10021. CARDEZA FDN HEMOPHILIA CTR,PHILADELPHIA,PA. MT SINAI MED CTR,DEPT HEMATOL,NEW YORK,NY 10029. NATL RETROVIRUS REFERENCE CTR,ATHENS,GREECE. UNIV ATHENS,SCH MED,ATHENS,GREECE. UNIV VIENNA,DEPT INTERNAL MED,A-1010 VIENNA,AUSTRIA. PENN STATE UNIV,MILTON S HERSHEY MED CTR,SCH MED,DIV HEMATOL,HERSHEY,PA 17033. UNIV N CAROLINA,SCH MED,DIV HEMATOL,CHAPEL HILL,NC 27514. GEORGE WASHINGTON UNIV,MED CTR,DIV HEMATOL ONCOL,WASHINGTON,DC 20037. CASE WESTERN RESERVE UNIV,SCH MED,DIV INFECT DIS,CLEVELAND,OH 44106. UNIV GENEVA,HEMOSTASIS UNIT,CH-1211 GENEVA 4,SWITZERLAND. HOP CANTONAL GENEVA,CH-1211 GENEVA 4,SWITZERLAND. CHILDRENS HOSP,NATL MED CTR,DEPT HEMATOL,WASHINGTON,DC 20010. CHILDRENS HOSP PHILADELPHIA,DIV HEMATOL,PHILADELPHIA,PA. TULANE UNIV,SCH MED,HEMATOL SECT,NEW ORLEANS,LA 70112. UNIV COLORADO,HLTH SCI CTR,DEPT PEDIAT HEMATOL,DENVER,CO 80262. UNIV MUNICH,MED CLIN,DIV HEMOSTASIS,W-8000 MUNICH 2,GERMANY. RES TRIANGLE INST,ROCKVILLE,MD. FU NCI NIH HHS [N01-CP-85649, N01-CO-74102, N01-CP-95663] NR 32 TC 110 Z9 111 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 26 PY 1992 VL 267 IS 8 BP 1090 EP 1094 DI 10.1001/jama.267.8.1090 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA HE391 UT WOS:A1992HE39100024 PM 1735926 ER PT J AU WU, XM GUTFREUND, H CHOCK, PB AF WU, XM GUTFREUND, H CHOCK, PB TI KINETIC METHOD FOR DIFFERENTIATING MECHANISMS FOR LIGAND-EXCHANGE REACTIONS - APPLICATION TO TEST FOR SUBSTRATE CHANNELING IN GLYCOLYSIS SO BIOCHEMISTRY LA English DT Article ID ENZYME-ENZYME COMPLEXES; LACTATE-DEHYDROGENASE; NADH AB We have derived analytical expressions for the kinetics of the two mechanisms involved in ligand substitution reactions. These mechanisms are (i) a dissociative mechanism in which the leaving ligand is first dissociated prior to the binding of the incoming ligand and (ii) an associative mechanism where a ternary complex is formed between the incoming ligand and the complex containing the leaving ligand. The equations obtained provide the theoretical basis for differentiating these two mechanisms on the basis of their kinetic patterns of the displacement reactions. Analysis of these equations shows that an associative mechanism can only generate an increasing kinetic pattern for the observed pseudo-first-ordered rate constants as a function of increasing concentration of the incoming ligand and plateaus, in most cases, at a value higher than the off-rate constant of the leaving ligand. However, a dissociative mechanism can generate either an increasing or a decreasing (k(app) decreases with increasing concentrations of the incoming ligand) kinetic pattern, depending on the magnitudes of the individual rate constants involved, and, in either case, it will plateau at k(app) equal to the k(off) of the leaving ligand. Therefore, the decreasing kinetic pattern is a hallmark for a dissociative mechanism. This general method was used to settle the dispute of whether NADH is transferred directly via the enzyme-enzyme complex between glycerol-3-phosphate dehydrogenase (GPDH; EC1.1.1.8) and L-lactate dehydrogenase (LDH; EC1.1.1.27). Evidence for direct transfer of this metabolite between these two enzymes has been claimed and used to support substrate channeling in glycolysis [Srivastava, D. K., & Bernhard, S. A. (1987) Biochemistry 26, 1240-1246; Srivastava, D. K., Smolen, P., Betts, G. F., Fukushima, T., Spivey, H. O., & Bernhard, S. A. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 6464-6468]. Reexamination of this evidence reveals that the conclusion is based on misinterpretation of the kinetics of ligand exchange [Chock, P. B., & Gutfreund, H. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8870-8874]. Application of this method to analyze the displacement data from our laboratory and those reported by Srivastava et al. (1989) confirms that NADH transfer between its complex with GPDH and with LDH proceeds via a dissociative mechanism. This is consistent with the transient kinetic and sedimentation equilibrium data reported by Wu et al. [Wu, X., Gutfreund, H., Lokatos, S., & Chock, P. B. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 497-501]. C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. UNIV BRISTOL,DEPT BIOCHEM,BRISTOL BS8 1TH,AVON,ENGLAND. NR 8 TC 11 Z9 11 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 25 PY 1992 VL 31 IS 7 BP 2123 EP 2128 DI 10.1021/bi00122a033 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HF267 UT WOS:A1992HF26700033 PM 1536852 ER PT J AU PITHA, J HOSHINO, T AF PITHA, J HOSHINO, T TI EFFECTS OF ETHANOL ON FORMATION OF INCLUSION COMPLEXES OF HYDROXYPROPYLCYCLODEXTRINS WITH TESTOSTERONE OR WITH METHYL-ORANGE SO INTERNATIONAL JOURNAL OF PHARMACEUTICS LA English DT Article DE CYCLODEXTRIN; HYDROXYPROPYLCYCLODEXTRIN; INCLUSION COMPLEXATION; SOLVENT EFFECT; TESTOSTERONE ID BETA-CYCLODEXTRIN; CRYSTAL-STRUCTURE; FLUORESCENCE; DERIVATIVES; SOLUBILITY; SOLVENTS; ALCOHOL; BINDING; MODEL AB Gradual additions of ethanol decreased and eventually abolished the formation of inclusion complexes of testosterone with hydroxypropylcyclodextrins in aqueous solutions. With hydroxypropyl-beta-cyclodextrin this occurred through two mechanisms. At low concentrations of ethanol (< 30%), the solvent primarily acted as a competing guest compound; at higher concentrations the dissociation primarily occurred through non-specific solvent effects. With hydroxypropyl-gamma-cyclodextrin only the dissociation through nonspecific solvent effects was observed. Surprisingly, when ethanolic solutions containing fully dissociated complexes were evaporated, the solid residues had properties characteristic of complexed species, i.e., they showed the rapid and complete dissolution characteristic of complexes prepared by freeze drying of aqueous solutions. That inclusion complexes were formed during the final stages of evaporation of ethanolic solution of components was confirmed by measurements of circular dichroic spectra of a methyl orange: hydroxypropyl-beta-cyclodextrin combination. In this combination the spectra of included species were highly characteristic and were recorded both in aqueous solutions and in solid state after the evaporation of ethanolic solutions but not in concentrated ethanolic solutions. RP PITHA, J (reprint author), NIA,GRC,BALTIMORE,MD 21224, USA. NR 22 TC 55 Z9 58 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5173 J9 INT J PHARM JI Int. J. Pharm. PD FEB 25 PY 1992 VL 80 IS 2-3 BP 243 EP 251 DI 10.1016/0378-5173(92)90281-6 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HK114 UT WOS:A1992HK11400016 ER PT J AU PITHA, J HOSHINO, T TORRESLABANDEIRA, J IRIE, T AF PITHA, J HOSHINO, T TORRESLABANDEIRA, J IRIE, T TI PREPARATION OF DRUG - HYDROXYPROPYLCYCLODEXTRIN COMPLEXES BY A METHOD USING ETHANOL OR AQUEOUS AMMONIUM HYDROXIDE AS CO-SOLUBILIZERS SO INTERNATIONAL JOURNAL OF PHARMACEUTICS LA English DT Article DE CYCLODEXTRIN; HYDROXYPROPYLCYCLODEXTRIN; COMPLEX PREPARATION; STEROID; GRAMICIDIN-S; AMPHOTERICIN-B; SOLUBILIZATION ID GAMMA-CYCLODEXTRIN; AMPHOTERICIN-B AB The dissolution of lipophilic drugs in aqueous solutions of hydroxypropylcyclodextrins can be accelerated by the addition of co-solubilizers such as ethanol or ammonia. These co-solubilizers can be removed later, together with water, by evaporation or freeze-drying, leaving drug: hydroxypropylcyclodextrin complexes. The co-solubilizer method was used successfully with steroid drugs (5-androstene-3-beta,17-beta-diol, 4-androstene-3,17-dione, dehydroepiandrosterone, dexamethasone, 5-alpha-dihydrotestosterone, 6-methylprednisolone and testosterone), peptides (gramicidin S) and a macrocyclic antibiotic (amphotericin B). The complexes prepared in this manner were amorphous and of satisfactory stability and solubility. C1 KUMAMOTO UNIV,FAC PHARMACEUT SCI,KUMAMOTO 860,JAPAN. RP PITHA, J (reprint author), NIA,GRC,BALTIMORE,MD 21224, USA. NR 15 TC 21 Z9 22 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5173 J9 INT J PHARM JI Int. J. Pharm. PD FEB 25 PY 1992 VL 80 IS 2-3 BP 253 EP 258 DI 10.1016/0378-5173(92)90282-7 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HK114 UT WOS:A1992HK11400017 ER PT J AU BEBENEK, K ROBERTS, JD KUNKEL, TA AF BEBENEK, K ROBERTS, JD KUNKEL, TA TI THE EFFECTS OF DNTP POOL IMBALANCES ON FRAMESHIFT FIDELITY DURING DNA-REPLICATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID RIBONUCLEOTIDE REDUCTASE; TRIPHOSPHATE POOLS; MAMMALIAN-CELLS; POLYMERASE-I; MUTAGENESIS; MUTATION; BACTERIOPHAGE-T4; MISALIGNMENT; SPECIFICITY; COMPLEX AB The use of unequal concentrations of the four deoxynucleoside triphosphates (dNTPs) in DNA polymerization reactions alters base substitution error rates in a predictable way. Less is known about the effects of substrate imbalances on base addition and deletion error rates. Thus, we examined pool bias effects on frameshift fidelity during DNA synthesis catalyzed by replicative DNA polymerases. Imbalanced pools altered the frameshift fidelity of the human immunodeficiency virus type-1 reverse transcriptase. Both mutagenic and antimutagenic effects were observed for minus-one, plus-one, and minus-two nucleotide errors, in a highly sequence-specific manner. Most of this specificity can be rationalized by either of two models. One involves frameshifts initiated by pool bias-induced nucleotide misinsertion, and the other involves pool bias-initiated template-primer slippage. Several examples of complex mutations were also recovered more than once in small mutant collections. These contained closely spaced single-base substitution and minus-one base frameshift changes. The two changes occurred at a frequency much higher than predicted if they were generated independently. This suggests that when the polymerase makes one mistake, the probability that it will make a second mistake within the next few incorporations increases significantly. Perturbation of dNTP pools also affected the frameshift fidelity of the replicative yeast DNA polymerase-alpha. In reactions containing a low concentration of one dNTP, the error rate increased for one-nucleotide deletions at homopolymeric template nucleotides complementary to the dNTP whose concentration was low. We extended this approach to determine the frameshift fidelity of simian virus 40 origin-dependent semiconservative replication of double-stranded DNA in extracts of human cells. In reactions performed with an equal concentration of all four dNTPs, replication was highly accurate for minus-one-nucleotide errors. However, when the concentration of one dNTP was decreased, the replication error rate increased at complementary, homopolymeric template positions. This response provides an approach for describing frameshift accuracy during replication of the leading and lagging strands. C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NR 30 TC 121 Z9 122 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 25 PY 1992 VL 267 IS 6 BP 3589 EP 3596 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE607 UT WOS:A1992HE60700010 PM 1371272 ER PT J AU LUYTEN, FP YU, YM YANAGISHITA, M VUKICEVIC, S HAMMONDS, RG REDDI, AH AF LUYTEN, FP YU, YM YANAGISHITA, M VUKICEVIC, S HAMMONDS, RG REDDI, AH TI NATURAL BOVINE OSTEOGENIN AND RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2B ARE EQUIPOTENT IN THE MAINTENANCE OF PROTEOGLYCANS IN BOVINE ARTICULAR-CARTILAGE EXPLANT CULTURES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID INDUCTIVE PROTEIN; BETA FAMILY; DIFFERENTIATION; CHROMATOGRAPHY; PURIFICATION; METABOLISM; ASSAY; ACID AB Osteogenin and related bone morphogenetic proteins are members of the transforming growth factor-beta superfamily, and were isolated by their ability to induce cartilage and bone formation in vivo. The influence of osteogenin, purified from bovine bone, and of recombinant human bone morphogenetic protein-2B (BMP-2B) has been examined in bovine articular cartilage explants. Both differentiation factors stimulated in a dose-dependent manner the synthesis of proteoglycans and decreased their rate of degradation. At a dose of 30 ng/ml, proteoglycan synthesis was increased to levels observed with either 20 ng/ml insulin-like growth factor 1, 10 ng/ml transforming growth factor-beta, or 20% fetal bovine serum. This increase of biosynthetic rates above basal medium levels was observed in young, adolescent, and adult tissues. Analysis of the size of the newly synthesized proteoglycans, the glycosaminoglycan chain size, and the glycosaminoglycan type of explants treated with osteogenin or BMP-2B were very comparable to each other, and to proteoglycans isolated from cartilage treated with either insulin-like growth factor I or fetal bovine serum. These results demonstrate that osteogenin and BMP-2B alone are capable of stimulating and maintaining the chondrocyte phenotype in vitro. C1 NIDR, PROTEOGLYCAN CHEM SECT, BETHESDA, MD 20892 USA. GENENTECH INC, DEPT DEV BIOL, S SAN FRANCISCO, CA 94080 USA. RP LUYTEN, FP (reprint author), NIDR, BUNE CELL BIOL SECT, BLDG 30, ROOM 211, BETHESDA, MD 20892 USA. NR 28 TC 121 Z9 125 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 25 PY 1992 VL 267 IS 6 BP 3691 EP 3695 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE607 UT WOS:A1992HE60700025 PM 1740421 ER PT J AU RAWLINGS, DJ KASLOW, DC AF RAWLINGS, DJ KASLOW, DC TI A NOVEL 40-KDA MEMBRANE-ASSOCIATED EF-HAND CALCIUM-BINDING PROTEIN IN PLASMODIUM-FALCIPARUM SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSMISSION-BLOCKING ANTIBODIES; AMINO-ACID-SEQUENCE; GEL-ELECTROPHORESIS; SEXUAL STAGES; ANTIGENS; DNA; NITROCELLULOSE; EXPRESSION; IMMUNITY; VACCINE AB A 40-kDa sexual stage radiolabeled surface protein of Plasmodium falciparum, Pfs40, was previously identified as a potential target antigen of transmission blocking immunity by an immunogenetic approach. Synthetic oligonucleotide "guessmers," based on micro-sequenced tryptic peptides of Pfs40 purified by two-dimensional gel electrophoresis, were used to clone the full length cDNA and genomic DNA encoding Pfs40. The deduced amino acid sequence predicted an integral membrane protein containing five EF-hand calcium-binding domains. The biological activity of one or more of these domains was confirmed by binding of Ca-45 to both native and recombinant Pfs40. Antisera to recombinant Pfs40 immunoprecipitated the native radiolabeled 40-kDa surface protein. The predicted noncytosolic membrane-associated localization of Pfs40 is unique within the EF-hand calcium-binding protein superfamily. C1 NIAID,PARASIT DIS LAB,MALARIA SECT,BLDG 4,ROOM B1-37,BETHESDA,MD 20892. NR 43 TC 52 Z9 53 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 25 PY 1992 VL 267 IS 6 BP 3976 EP 3982 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE607 UT WOS:A1992HE60700066 PM 1740445 ER PT J AU EMMERICH, J BEG, OU PETERSON, J PREVIATO, L BRUNZELL, JD BREWER, HB SANTAMARINAFOJO, S AF EMMERICH, J BEG, OU PETERSON, J PREVIATO, L BRUNZELL, JD BREWER, HB SANTAMARINAFOJO, S TI HUMAN LIPOPROTEIN-LIPASE - ANALYSIS OF THE CATALYTIC TRIAD BY SITE-DIRECTED MUTAGENESIS OF SER-132, ASP-156, AND HIS-241 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID POST-HEPARIN PLASMA; PANCREATIC LIPASE; MOLECULAR-CLONING; HEPATIC LIPASE; GENE FAMILY; SEQUENCE; CDNA; BINDING; INACTIVATION; DEFICIENCY AB Lipoprotein lipase (LPL) plays a central role in normal lipid metabolism as the key enzyme involved in the hydrolysis of triglycerides present in chylomicrons and very low density lipoproteins. LPL is a member of a family of hydrolytic enzymes that include hepatic lipase and pancreatic lipase. Based on primary sequence homology of LPL to pancreatic lipase, Ser- 132, Asp-156, and His-241 have been proposed to be part of a domain required for normal enzymic activity. We have analyzed the role of these potential catalytic residues by site-directed mutagenesis and expression of the mutant LPL in human embryonic kidney-293 cells. Substitution of Ser- 132, Asp- 156, and His-241 by several different residues resulted in the expression of an enzyme that lacked both triolein and tributyrin esterase activities. Mutation of other conserved residues, including Ser-97, Ser-307, Asp-78, Asp-37 1, Asp-440, His-93, and His-439 resulted in the expression of active enzymes. Despite their effect on LPL activity, substitutions of Ser-132, Asp-156, and His-241 did not change either the heparin affinity or lipid binding properties of the mutant LPL. In summary, mutation of Ser-132, Asp-156, and His-241 specifically abolishes total hydrolytic activity without disrupting other important functional domains of LPL. These combined results strongly support the conclusion that Ser-132, Asp-156, and His-241 form the catalytic triad of LPL and are essential for LPL hydrolytic activity. C1 NHLBI,MOLEC DIS BRANCH,BLDG 10,RM 7N117,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. FU NHLBI NIH HHS [HL30086] NR 35 TC 75 Z9 79 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 25 PY 1992 VL 267 IS 6 BP 4161 EP 4165 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE607 UT WOS:A1992HE60700087 PM 1371284 ER PT J AU SPITKOVSKY, DD ROYERPOKORA, B DELIUS, H KISSELJOV, F JENKINS, NA GILBERT, DJ COPELAND, NG ROYER, HD AF SPITKOVSKY, DD ROYERPOKORA, B DELIUS, H KISSELJOV, F JENKINS, NA GILBERT, DJ COPELAND, NG ROYER, HD TI TISSUE RESTRICTED EXPRESSION AND CHROMOSOMAL LOCALIZATION OF THE YB-1 GENE ENCODING A 42KD NUCLEAR CCAAT BINDING-PROTEIN SO NUCLEIC ACIDS RESEARCH LA English DT Article ID RNA POLYMERASE-II; DNA-REPLICATION; DEVELOPMENTAL REGULATION; HETEROLOGOUS SUBUNITS; TRANSCRIPTION FACTORS; ACCURATE INITIATION; MULTIPLE FACTORS; Y-BOX; PROMOTER; CLONING AB YB-1 cDNA clones were isolated by binding site screening of a Hela expression library using a human papillomavirus type 18 enhancer oligonucleotide. YB-1 belongs to a family of transcription factors which bind to recognition sequences containing a core CCAAT element. YB-1 bound to its single stranded recognition sequence on the sense strand but not to the anti-sense strand. A synthetic peptide antiserum derived from the predicted YB-1 amino acid sequence identified a 42kD nuclear protein in immunoblots. A protein with the same size was detected by binding site blotting experiments using the HPV18 enhancer oligonucleotide which bound YB-1. YB-1 gene expression was restricted in tissues from a human 24 week old fetus. High levels of YB-1 mRNA were present in heart, muscle, liver, lung, adrenal gland and the brain, in contrast, low amounts of YB-1 mRNA were found in thymus, kidney, bone marrow and spleen. In pancreas, bladder, stomach and testis YB-1 mRNA could not be detected by Northern hybridization. Finally, we have identified four YB-1 related loci in the mouse genome and have mapped these loci to four different mouse chromosomes by interspecific backcross analysis. C1 DEUTSCH KREBSFORSCHUNGSZENTRUM,ANGEW TUMORVIROL,NEVENHEIMER FELD 506,W-6900 HEIDELBERG,GERMANY. UNIV HEIDELBERG,INST HUMAN GENET & ANTHROPOL,W-6900 HEIDELBERG,GERMANY. ALL UNION CANC RES CTR,MOSCOW 115478,USSR. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702. NR 44 TC 52 Z9 52 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 25 PY 1992 VL 20 IS 4 BP 797 EP 803 DI 10.1093/nar/20.4.797 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG350 UT WOS:A1992HG35000022 PM 1542575 ER PT J AU BRESNICK, EH RORIES, C HAGER, GL AF BRESNICK, EH RORIES, C HAGER, GL TI EVIDENCE THAT NUCLEOSOMES ON THE MOUSE MAMMARY-TUMOR VIRUS PROMOTER ADOPT SPECIFIC TRANSLATIONAL POSITIONS SO NUCLEIC ACIDS RESEARCH LA English DT Article ID DNA INTERACTIONS; YEAST; CHROMATIN; TRANSCRIPTION; SEQUENCE; INVITRO; BINDING; INVIVO AB We have previously demonstrated that an array of six nucleosomes are phased on the mouse mammary tumor virus (MMTV) long terminal repeat (1,2). In this study, we devised a new assay to measure the translational positions of specific nucleosomes on the MMTV promoter. Nucleosome core particles were purified and shown to contain A and B nucleosomal DNA by Taq polymerase primer extension with nucleosome-specific primers. The 5' and 3' boundaries of A and B nucleosomes were measured by extending to the end of the core DNA with internal primers. This approach yielded results consistent with major translational positions of -23 to +123 and -221 to -75 for A and B nucleosomes, respectively. The micrococcal nuclease cleavage patterns of A and B nucleosome regions in isolated nuclei are conserved at base-pair resolution in multiple murine cell lines containing either stable MMTV-reporter chimeras or endogenous proviruses. As the refined nucleosome positions place important transcription factor binding sites at the 3' edge of the B nucleosome and in the nucleosome A/B linker, we propose that linker histone depletion and chromatin unfolding may be required to expose these cis-elements during steroid hormone-induced transcription initiation. C1 NCI,MOLEC VIROL LAB,HORMONE ACT & ONCOGENESIS SECT,BETHESDA,MD 20892. NR 25 TC 42 Z9 42 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 25 PY 1992 VL 20 IS 4 BP 865 EP 870 DI 10.1093/nar/20.4.865 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG350 UT WOS:A1992HG35000032 PM 1311832 ER PT J AU BHATIA, K GUTIERREZ, MI HUPPI, K MAGRATH, IT AF BHATIA, K GUTIERREZ, MI HUPPI, K MAGRATH, IT TI PCR DETECTION OF A NEUTRAL CGA/CGG DIMORPHISM IN EXON-6 OF THE HUMAN P53 GENE SO NUCLEIC ACIDS RESEARCH LA English DT Note RP BHATIA, K (reprint author), NCI,BETHESDA,MD 20892, USA. NR 2 TC 22 Z9 22 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 25 PY 1992 VL 20 IS 4 BP 928 EP 928 DI 10.1093/nar/20.4.928-a PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG350 UT WOS:A1992HG35000063 PM 1542597 ER PT J AU MUZZIN, P REVELLI, JP FRASER, CM GIACOBINO, JP AF MUZZIN, P REVELLI, JP FRASER, CM GIACOBINO, JP TI RADIOLIGAND BINDING-STUDIES OF THE ATYPICAL BETA-3-ADRENERGIC RECEPTOR IN RAT BROWN ADIPOSE-TISSUE USING [H-3] CGP-12177 SO FEBS LETTERS LA English DT Article DE ATYPICAL BETA-ADRENERGIC RECEPTOR; BROWN FAT; BINDING ID BETA-ADRENERGIC-RECEPTOR; THERMOGENESIS; ADIPOCYTES; CGP-12177; AGONIST AB Two populations of [H-3]CGP 12177 binding sites exist in rat interscapular brown adipose tissue (IBAT) plasma membranes. The majority of binding sites are of low affinity with a K(d) of 31 nM, a value in close agreement with that for the K(d) of [H-3]CGP 12177 binding to a cloned rat beta-3-adrenergic receptor (AR) expressed in CHO cells (44 nM). Competition binding studies demonstrate that the K(i) values of the cloned rat beta-3-AR and of the low affinity sites in IBAT are 45 and 29 nM, respectively, for BRL 37344 and 1.4 and 1.0-mu-M, for (-)-propranolol. These findings strongly suggest that the low affinity [H-3]CGP 12177 binding site measured in IBAT plasma membranes represents the atypical beta-3-AR in this tissue. C1 NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,MOLEC NEUROBIOL SECT,ROCKVILLE,MD 20852. RP MUZZIN, P (reprint author), UNIV GENEVA,MED CTR,DEPT BIOCHIM MED,1 MICHEL SERVET,CH-1211 GENEVA 4,SWITZERLAND. NR 14 TC 58 Z9 58 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD FEB 24 PY 1992 VL 298 IS 2-3 BP 162 EP 164 DI 10.1016/0014-5793(92)80046-J PG 3 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA HG045 UT WOS:A1992HG04500015 PM 1347503 ER PT J AU MILDVAN, D MACHADO, SG WILETS, I GROSSBERG, SE AF MILDVAN, D MACHADO, SG WILETS, I GROSSBERG, SE TI ENDOGENOUS INTERFERON AND TRIGLYCERIDE CONCENTRATIONS TO ASSESS RESPONSE TO ZIDOVUDINE IN AIDS AND ADVANCED AIDS-RELATED COMPLEX SO LANCET LA English DT Article ID TUMOR NECROSIS FACTOR; GAMMA AB To improve evaluation of new antiretroviral drugs in the acquired immunodeficiency syndrome (AIDS), sensitive biological markers that accurately predict response to treatment are needed. Two possible markers are endogenous interferon (E-IFN), which is a cytokine involved in the pathophysiology of AIDS, and serum triglycerides (TG), which are raised, in patients with AIDS, possibly reflecting enhanced cytokine activity. E-IFN, TG, body-mass index, CD4 count, and HIV p24 were measured in 19 patients (15 with AIDS, 4 with AIDS-related complex), who were part of the phase II licensing trial of zidovudine (ZDV). 10 received ZDV and 9 received placebo. Rapid, significant, and sustained declines from initial values in E-IFN and TG concentrations were observed in ZDV patients but not in placebo patients. Baseline values of E-IFN and TG concentrations after 4 months on ZDV treatment were both important contributors to long-term survival. The findings suggest that these indicators of abnormal cytokine expression may be useful measures of not only disease severity but also efficacy of antiretroviral therapy in AIDS. C1 BETH ISRAEL MED CTR,INST CHEM DEPENDENCY,NEW YORK,NY 10003. CUNY MT SINAI SCH MED,NEW YORK,NY 10029. NIAID,DIV AIDS,BIOSTAT RES BRANCH,BETHESDA,MD 20892. MED COLL WISCONSIN,DEPT MICROBIOL,MILWAUKEE,WI 53226. RP MILDVAN, D (reprint author), BETH ISRAEL MED CTR,DIV INFECT DIS,1ST AVE & 16TH ST,NEW YORK,NY 10003, USA. NR 19 TC 84 Z9 84 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD FEB 22 PY 1992 VL 339 IS 8791 BP 453 EP 456 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA HE932 UT WOS:A1992HE93200004 PM 1371323 ER PT J AU MIZUGUCHI, M YAMADA, M RHEE, SG KIM, SU AF MIZUGUCHI, M YAMADA, M RHEE, SG KIM, SU TI DEVELOPMENT OF INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE IMMUNOREACTIVITY IN CEREBELLAR PURKINJE-CELLS INVIVO AND INVITRO SO BRAIN RESEARCH LA English DT Article DE PHOSPHOINOSITIDE 2ND MESSENGER SYSTEM; INOSITOL 1,4,5-TRISPHOSPHATE; INOSITOL 1,3,4,5-TETRAKISPHOSPHATE; IMMUNOHISTOCHEMISTRY; PURKINJE CELL; TISSUE CULTURE ID RAT-BRAIN; TRISPHOSPHATE; PURIFICATION; TETRAKISPHOSPHATE; MESSENGER; TISSUES AB Development profiles in vivo and in vitro of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated immunohistochemically in the cerebellar Purkinje cells. In in vivo preparations of rat cerebellum, IP3K immunoreactivity appeared in Purkinje cell bodies and dendrites shortly after birth, increased rapidly by postnatal day 5, and was subsequently confined to their dendritic processes by day 20. The appearance and shift of IP3K immunoreactivity in Purkinje cells showed an identical time course even when Purkinje cells were placed under culture conditions commencing on day 0, suggesting that Purkinje cells have their own biological clock on the expression of IP3K in the absence of external influences. C1 UNIV BRITISH COLUMBIA,DEPT MED,DIV NEUROL,VANCOUVER V6T 1W5,BC,CANADA. NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 18 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 21 PY 1992 VL 573 IS 1 BP 157 EP 160 DI 10.1016/0006-8993(92)90126-T PG 4 WC Neurosciences SC Neurosciences & Neurology GA HH574 UT WOS:A1992HH57400019 PM 1315605 ER PT J AU OYLER, GA POLLI, JW HIGGINS, GA WILSON, MC BILLINGSLEY, ML AF OYLER, GA POLLI, JW HIGGINS, GA WILSON, MC BILLINGSLEY, ML TI DISTRIBUTION AND EXPRESSION OF SNAP-25 IMMUNOREACTIVITY IN RAT-BRAIN, RAT PC-12 CELLS AND HUMAN SMS-KCNR NEUROBLASTOMA-CELLS SO DEVELOPMENTAL BRAIN RESEARCH LA English DT Article DE IMMUNOCYTOCHEMISTRY; DEVELOPMENT; DIFFERENTIATION; RETINOIC ACID; SYNAPTOGENESIS ID NERVE GROWTH-FACTOR; HUMAN NEURO-BLASTOMA; RETINOIC ACID; PROTEINS; DIFFERENTIATION; BINDING; TRACT AB Immunocytochemical, immunoblotting and in situ hybridization studies were used to map the distribution of SNAP-25 protein and mRNA in the rodent nervous system. These experiments demonstrated that subsets of neurons expressed SNAP-25, and that several patterns of expression emerged: SNAP-25 expression in caudate nucleus was initially concentrated in axons, which subsequently was localized in presynaptic regions of these axons. Other regions, typified by neocortex, showed developmental increases and persistent adult neuronal immunoreactivity for SNAP-25. Finally, olfactory bulb contained neurons which initially expressed SNAP-25, but lost expression during maturation. Additional studies in cultured human and rat cell lines derived from neural crest suggested that SNAP-25 is expressed in such lines, but not in glial or fibroblast lines. Differentiation of rat PC-12 cells with nerve growth factor failed to alter steady-state levels of SNAP-25 protein; similar responses were seen in human SMS-KCNR neuroblastoma cells differentiated using retinoic acid. The presence of SNAP-25 in presynaptic regions of numerous neuronal subsets and in neural crest cell lines suggests that this protein subserves an important function in neuronal tissues. C1 PENN STATE UNIV, MILTON S HERSHEY MED CTR,COLL MED,DEPT PHARMACOL, POB 850, HERSHEY, PA 17033 USA. PENN STATE UNIV, MILTON S HERSHEY MED CTR, COLL MED, CTR CELL & MOLEC BIOL, HERSHEY, PA 17033 USA. NIA, GERONTOL RES CTR, BALTIMORE, MD 21224 USA. Scripps Res Inst, RES INST, DEPT NEUROPHARMACOL, LA JOLLA, CA 92037 USA. NR 28 TC 44 Z9 44 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-3806 J9 DEV BRAIN RES JI Dev. Brain Res. PD FEB 21 PY 1992 VL 65 IS 2 BP 133 EP 146 DI 10.1016/0165-3806(92)90172-S PG 14 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA HH181 UT WOS:A1992HH18100001 ER PT J AU KOHN, MC AF KOHN, MC TI PROPAGATION OF INFORMATION IN METANET GRAPH MODELS SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article ID SENSITIVITY RP KOHN, MC (reprint author), NIEHS,STAT & BIOMATH BRANCH,POB 12233,RES TRIANGLE PK,NC 27710, USA. FU NCRR NIH HHS [RR 01693] NR 17 TC 2 Z9 2 U1 0 U2 1 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD FEB 21 PY 1992 VL 154 IS 4 BP 505 EP 517 DI 10.1016/S0022-5193(05)80166-3 PG 13 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA HF459 UT WOS:A1992HF45900008 PM 1593899 ER PT J AU MICKLUS, MJ GREIG, NH TUNG, J RAPOPORT, SI AF MICKLUS, MJ GREIG, NH TUNG, J RAPOPORT, SI TI ORGAN DISTRIBUTION OF LIPOSOMAL FORMULATIONS FOLLOWING INTRACAROTID INFUSION IN RATS SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE BLOOD-BRAIN BARRIER; LIPOSOME; MANNOPYRANOSIDE; SULFATIDE; CATIONIZED BOVINE SERUM ALBUMIN ID BLOOD-BRAIN-BARRIER; TRANSPORT; PEPTIDE AB The blood-brain barrier (BBB) limits the ability of many hydrophilic solutes to pass from the circulatory system into the nervous system and significantly restricts brain drug delivery. This study examined brain uptake of liposomal formulations in rats. Radiolabeled liposomes less than 1-mu-m in diameter, prepared from phosphatidylcholine, cholesterol and containing p-aminophenyl-alpha-mannopyranoside or sulfatide, or covalently linked to cationized albumin were injected into the right carotid arteries of rats, and organ distribution determined 10 min and 1 h after injection. In all formulations, liposomal retention in brain did not exceed one-tenth of 1% of the injected dose. It seems unlikely that liposomes can significantly bind to or cross the BBB; however, apparent brain uptake could result from liposomal entrapment in the small capillaries of the brain by liposomes larger than 1-mu-m in diameter. C1 DREXEL UNIV,INST BIOMED ENGN & SCI,PHILADELPHIA,PA 19104. RP MICKLUS, MJ (reprint author), NIA,NEUROSCI LAB,BETHESDA,MD 20892, USA. NR 24 TC 22 Z9 23 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD FEB 20 PY 1992 VL 1124 IS 1 BP 7 EP 12 DI 10.1016/0005-2760(92)90118-F PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HH573 UT WOS:A1992HH57300002 PM 1543728 ER PT J AU CLORE, GM GRONENBORN, AM AF CLORE, GM GRONENBORN, AM TI LOCALIZATION OF BOUND WATER IN THE SOLUTION STRUCTURE OF THE IMMUNOGLOBULIN BINDING DOMAIN OF STREPTOCOCCAL PROTEIN-G - EVIDENCE FOR SOLVENT-INDUCED HELICAL DISTORTION IN SOLUTION SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Note DE IGG BINDING DOMAIN; BOUND WATER; SOLUTION STRUCTURE; 3D-HETERONUCLEAR NMR; ROE ID ROTATING-FRAME; NMR; SPECTROSCOPY RP CLORE, GM (reprint author), NIDDKD,CHEM PHYS LAB,BLDG 2,BETHESDA,MD 20892, USA. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 16 TC 47 Z9 47 U1 0 U2 2 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1992 VL 223 IS 4 BP 853 EP 856 DI 10.1016/0022-2836(92)90247-H PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HG601 UT WOS:A1992HG60100006 PM 1538400 ER PT J AU CHABNER, BA FRIEDMAN, MA AF CHABNER, BA FRIEDMAN, MA TI PROGRESS AGAINST RARE AND NOT-SO-RARE CANCERS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material ID CELL LUNG-CANCER; CHEMOTHERAPY; RADIATION; LEUKEMIA RP CHABNER, BA (reprint author), NCI,BETHESDA,MD 20892, USA. NR 12 TC 4 Z9 4 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD FEB 20 PY 1992 VL 326 IS 8 BP 563 EP 565 DI 10.1056/NEJM199202203260811 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA HD658 UT WOS:A1992HD65800011 PM 1310161 ER PT J AU JABLON, S BOICE, JD AF JABLON, S BOICE, JD TI REPORTING NEGATIVE STUDIES IN THE MASS-MEDIA SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP JABLON, S (reprint author), NCI,ROCKVILLE,MD, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 19 PY 1992 VL 267 IS 7 BP 931 EP 931 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA HD155 UT WOS:A1992HD15500012 PM 1734098 ER PT J AU HAYES, HM TARONE, RE CANTOR, KP AF HAYES, HM TARONE, RE CANTOR, KP TI CANINE MALIGNANT-LYMPHOMA AND 2,4-DICHLOROPHENOXYACETIC ACID HERBICIDES - RESPONSE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter RP HAYES, HM (reprint author), NCI,DIV CANC ETIOL,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892, USA. NR 7 TC 0 Z9 0 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 19 PY 1992 VL 84 IS 4 BP 271 EP 271 DI 10.1093/jnci/84.4.271-a PG 1 WC Oncology SC Oncology GA HG728 UT WOS:A1992HG72800016 ER PT J AU CHUANG, LF KUNG, HF ISRAEL, M CHUANG, RY AF CHUANG, LF KUNG, HF ISRAEL, M CHUANG, RY TI ACTIVATION OF HUMAN LEUKEMIA PROTEIN-KINASE-C BY TUMOR PROMOTERS AND ITS INHIBITION BY N-TRIFLUOROACETYLADRIAMYCIN-14-VALERATE (AD32) SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID RNA-POLYMERASE; PHORBOL ESTER; POTENT INHIBITOR; CYTO-TOXICITY; ADRIAMYCIN; DNA; CELLS; INVITRO; COMPLEX; LYMPHOMA AB N-Trifluoroacetyladriamycin-14-valerate (AD 32), a lipophilic, DNA non-binding analog of Adriamycin(R) (ADR), was found to be a potent inhibitor of the membrane-bound enzyme, protein kinase C (PKC). PKC was isolated and purified from human leukemia ML-1 cells, and the enzyme activity was shown to be activated by the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-dibutyrate (PDBu). AD-32, nevertheless, inhibited the activation of PKC by TPA or PDBu. The IC50 values for AD 32 inhibition of PKC activation were 0.85-mu-M for TPA and 1.25-mu-M for PDBu. Under the same assay conditions, ADR demonstrated much higher IC50 values: 550-mu-M for TPA and > 350-mu-M for PDBu. The inhibition of PKC by AD 32 was further shown to be competitive in nature; AD 32 inhibited the binding of [H-3]PDBu to PKC. Therefore, AD 32 competes with the tumor promoter for the PKC binding site and prevents the latter from both interacting with the phospholipid and binding to PKC. These effects of AD 32 were reproduced in situ; incubation of human leukemia ML-1 cells with TPA showed an increased phosphorylation of cellular proteins, and the TPA-induced protein phosphorylation was inhibited by the addition of AD 32 to the cultured cells. C1 UNIV CALIF DAVIS,DEPT MED PHARMACOL & TOXICOL,DAVIS,CA 95616. NCI,BIOCHEM PHYSIOL LAB,FREDERICK,MD 21701. UNIV TENNESSEE CTR HLTH SCI,DEPT PHARMACOL & MED CHEM,MEMPHIS,TN 38163. FU NCI NIH HHS [CA 37082]; NIDA NIH HHS [DA 05901] NR 33 TC 16 Z9 16 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD FEB 18 PY 1992 VL 43 IS 4 BP 865 EP 872 DI 10.1016/0006-2952(92)90254-G PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HG422 UT WOS:A1992HG42200028 PM 1540240 ER PT J AU BAUMGOLD, J NIKODIJEVIC, O JACOBSON, KA AF BAUMGOLD, J NIKODIJEVIC, O JACOBSON, KA TI PENETRATION OF ADENOSINE ANTAGONISTS INTO MOUSE-BRAIN AS DETERMINED BY EXVIVO BINDING SO BIOCHEMICAL PHARMACOLOGY LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; AMINE CONGENER; RAT-BRAIN; RECEPTORS; POTENT; METHYLXANTHINES; INHIBITION; MEMBRANES; ANALOGS AB The penetration of the adenosine antagonists 8-cyclopentyl-1,3-dimethylxanthine (CPT), 8-cyclopentyl-1,3-dipropylxanthine (CPX), 8-(p-sulfophenyltheophylline (8-PST), and 8-[4-[[[[(2-amino-ethyl) amino]carbonyl]methyl]oxy]phenyl]-1,3-dipropylxanthine (XAC) into mouse brain was determined using ex vivo binding and locomotor studies. CPT and CPX (25 and 0.25 mg/kg, respectively) both penetrated into brain in substantial amounts: 49 and 17% of theoretical levels assuming free penetration throughout the body, 10 min after i.p. injection, respectively. Brain levels of CPT decreased rapidly, declining to undetectable levels by 30 min post-injection, whereas levels of CPX declined much more slowly. As expected, no detectable brain levels of 8-PST were found following i.p. injection of 50 mg/kg. XAC (20 mg/kg) penetrated into brain poorly: 1.6% after 10 min and 3.2% 20 min post-injection. The ability of CPT to stimulate locomotor activity paralleled the brain levels, i.e. it was similar to theophylline at short times and the effect rapidly diminished. These studies demonstrate the usefulness of ex vivo binding in determining CNS penetration of adenosine receptor ligands. C1 GEORGE WASHINGTON UNIV,DEPT PHARMACOL & RADIOL,WASHINGTON,DC 20037. NIDDK,BIOORGAN CHEM LAB,BETHESDA,MD 20892. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20] NR 21 TC 61 Z9 61 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD FEB 18 PY 1992 VL 43 IS 4 BP 889 EP 894 DI 10.1016/0006-2952(92)90257-J PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HG422 UT WOS:A1992HG42200031 PM 1540242 ER PT J AU KLEINER, DE UNSWORTH, EJ KRUTZSCH, HC STETLERSTEVENSON, WG AF KLEINER, DE UNSWORTH, EJ KRUTZSCH, HC STETLERSTEVENSON, WG TI HIGHER-ORDER COMPLEX-FORMATION BETWEEN THE 72-KILODALTON TYPE-IV COLLAGENASE AND TISSUE INHIBITOR OF METALLOPROTEINASES-2 SO BIOCHEMISTRY LA English DT Article ID INTERSTITIAL COLLAGENASE; FIBROBLAST COLLAGENASE; CELL-LINES; ACTIVATION; TIMP-2; PURIFICATION; STROMELYSIN; EXPRESSION; PROTEINS AB The collagenases are a class of matrix degradative enzymes whose actions are important in physiological and pathological processes. The human 72-kDa type IV collagenase (matrix metalloproteinase-2) and its proteinase inhibitor, tissue inhibitor of metalloproteinases-2 (TIMP-2), are produced as a proenzyme-inhibitor complex by numerous cell lines. We analyzed the quaternary structure of and enzyme-inhibitor interactions in the native enzyme-inhibitor complex by studying the pattern of complexes demonstrated by molecular weight determination in nondenaturing polyacrylamide gels and evaluating the products formed by reaction of the native complexes with cross-linking agents. Electrophoresis in native polyacrylamide gels demonstrates that approximately 79% of the latent enzyme is present in a 1:1 bimolecular complex with the inhibitor TIMP-2, with 21% present as a complete tetrameric complex of two molecules of collagenase combined with two molecules of TIMP-2. The enzyme complex activated with organomercurials displays a shift to a higher proportion of the bimolecular complex with only 5% present as higher molecular weight complexes. Cross-linking of the latent and active forms of the complex with bis(sulfosuccinimidyl) suberate (BS3) and bis(sulfosuccinimidyl) tartarate demonstrates both the 1:1 and 2:2 complexes as well as an intermediate form that appears to be a complex composed of two molecules of collagenase and one of TIMP-2. The distribution of cross-linked products is unchanged with the addition of excess TIMP-2 to the reaction mix, implying that the binding sites for TIMP-2 to the initial enzyme-inhibitor complex are all occupied when the stoichiometry is 1 to 1. Cross-linking of the latent complex does not prevent organomercurial-induced activation and loss of an 80 amino acid prosegment. These cross-linked, activated complexes retain the ability to degrade gelatin in radiolabeled gelatin assays with the BS3-cross-linked, activated complex having a 7-fold higher specific activity than the un-cross-linked control. The BS3-cross-linked, activated complex is resistant to inhibition by free, exogenous TIMP-2. These results strongly suggest that there are two binding sites for TIMP-2 on collagenase and that binding to one site may decrease the affinity of binding of TIMP-2 to the second site. C1 NCI,PATHOL LAB,BLDG 10,ROOM 2A33,BETHESDA,MD 20892. RI Stetler-Stevenson, William/H-6956-2012; OI Stetler-Stevenson, William/0000-0002-5500-5808; Kleiner, David/0000-0003-3442-4453 NR 30 TC 64 Z9 65 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 18 PY 1992 VL 31 IS 6 BP 1665 EP 1672 DI 10.1021/bi00121a013 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE194 UT WOS:A1992HE19400013 PM 1310615 ER PT J AU PRING, M WEBER, A BUBB, MR AF PRING, M WEBER, A BUBB, MR TI PROFILIN ACTIN COMPLEXES DIRECTLY ELONGATE ACTIN-FILAMENTS AT THE BARBED END SO BIOCHEMISTRY LA English DT Article ID LOW-MOLECULAR WEIGHT; NON-MUSCLE CELLS; ACANTHAMOEBA PROFILIN; BINDING-PROTEIN; ATP HYDROLYSIS; F-ACTIN; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; POLYMORPHONUCLEAR LEUKOCYTES; DEPOLYMERIZING PROTEIN; BLOOD-PLATELETS AB We demonstrate that the profilin-G-actin complex can elongate actin filaments directly at the barbed end but cannot bind to the pointed end. During elongation, the profilin-actin complex binds to the barbed filament end, whereupon profilin is released, leaving the actin molecule behind. This was first proposed by Tilney [Tilney, L. G., et al. (1983) J. Cell Biol. 97, 112-124] and demonstrated by Pollard and Cooper [(1984) Biochemistry 23, 6631-6641] by electron microscopy. We show that a model without any outside energy supply, in contrast to the mechanism proposed by Pollard and Cooper, can be fitted to our and their [Kaiser et al. (1986) J. Cell Biol. 102, 221-226] findings. Input of outside energy is necessary only if profilin-mediated elongation continues after free G-actin has been lowered to or below the critical concentration observed at the barbed end in the absence of profilin. C1 UNIV PENN, DEPT BIOCHEM & BIOPHYS, PHILADELPHIA, PA 19104 USA. NHLBI, CELL BIOL LAB, BETHESDA, MD 20892 USA. UNIV PENN, DEPT PHYSIOL, PHILADELPHIA, PA 19104 USA. FU NHLBI NIH HHS [HL15835] NR 60 TC 108 Z9 108 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 18 PY 1992 VL 31 IS 6 BP 1827 EP 1836 DI 10.1021/bi00121a035 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE194 UT WOS:A1992HE19400035 PM 1737036 ER PT J AU TOUCHETTE, NA COLE, RD AF TOUCHETTE, NA COLE, RD TI EFFECTS OF SALT CONCENTRATION AND H1 HISTONE REMOVAL ON THE DIFFERENTIAL SCANNING CALORIMETRY OF NUCLEI SO BIOCHEMISTRY LA English DT Article ID THERMAL-DENATURATION; CHROMATIN FRAGMENTS; IONIC-STRENGTH; CORE PARTICLES; DNA; NUCLEOSOMES; PROTEINS; CELLS AB The effects of increasing NaCl concentrations on the melting profiles of chromatin in isolated nuclei contradicted published claims that structural transitions near 76-degrees-C (Tn-7), near 89-degrees-C (Tn-8), and near 105-degrees-C (Tn-10) were respectively the melting of linker DNA, the melting of extended nucleosomal strands, and the collapse of nucleosomes in the 300-angstrom fiber. Contrary to expectations of such an interpretation, decreases in salt concentration stabilized Tn-7 and failed to eliminate Tn-10. Moreover, nuclei depleted of H1 histone, which is known to be essential for the formation of the 300-angstrom fiber, gave the same melting profile as intact nuclei with regard to the relative magnitudes of Tn-8 and Tn-10. The effect of salt concentration on the melting profiles and the insensitivity of Tn-8 and Tn-10 to H1 histone removal supports the notion that Tn-7 is the collapse of the nucleosome while Tn-8 and Tn-10 are respectively the unstacking of nucleotide bases in relaxed chromatin and supercoiled chromatin. The identification of Tn-8 as the unstacking of bases in relaxed DNA, and Tn- 1 0 as unstacking in supercoiled DNA, shows that scanning calorimetry can be used to measure the state of repair of DNA in the nucleus. The gain in Tn-8 at the expense of Tn-10 that is seen as the mitotic index drops and differentiation occurs suggests that nicks accumulate in the DNA, perhaps because the gross aggregation of the inactive majority of the chromatin makes it inaccessible to repair enzymes. C1 JOURNAL NIH RES,2101 L ST NW,WASHINGTON,DC 20037. UNIV CALIF BERKELEY,MCB STANLEY DONNER ASU,BERKELEY,CA 94720. FU NIEHS NIH HHS [ES-01896]; NIGMS NIH HHS [GM20338] NR 31 TC 10 Z9 10 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 18 PY 1992 VL 31 IS 6 BP 1842 EP 1849 DI 10.1021/bi00121a037 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE194 UT WOS:A1992HE19400037 PM 1737037 ER PT J AU FLETCHER, TS KWEE, IL NAKADA, T LARGMAN, C MARTIN, BM AF FLETCHER, TS KWEE, IL NAKADA, T LARGMAN, C MARTIN, BM TI DNA-SEQUENCE OF THE YEAST TRANSKETOLASE GENE SO BIOCHEMISTRY LA English DT Article ID SACCHAROMYCES-CEREVISIAE; PENTOSE BIOSYNTHESIS; HYBRIDIZATION PROBES; CARBOXYL GROUP; ACTIVE-CENTER; BAKERS-YEAST; ENZYMES AB Transketolase (EC 2.2.1.1) is the enzyme that, together with aldolase, forms a reversible link between the glycolytic and pentose phosphate pathways. We have cloned and sequenced the transketolase gene from yeast (Saccharomyces cerevisiae). This is the first transketolase gene of the pentose phosphate shunt to be sequenced from any source. The molecular mass of the proposed translated protein is 73 976 daltons, in good agreement with the observed molecular mass of about 75 000 daltons. The 5'-nontranslated region of the gene is similar to other yeast genes. There is no evidence of 5'-splice junctions or branch points in the sequence. The 3'-nontranslated region contains the polyadenylation signal (AATAAA), 80 base pairs downstream from the termination codon. A high degree of homology is found between yeast transketolase and dihydroxyacetone synthase (formaldehyde transketolase) from the yeast Hansenula polymorpha. The overall sequence identity between these two proteins is 37%, with four regions of much greater similarity. The regions from amino acid residues 98-131, 157-182, 410-433, and 474-489 have sequence identities of 74%, 66%, 83%, and 82%, respectively. One of these regions (157-182) includes a possible thiamin pyrophosphate (TPP) binding domain, and another (410-433) may contain the catalytic domain. C1 VET AFFAIRS MED CTR,NEUROCHEM RES LAB,MARTINEZ,CA 94553. UNIV CALIF DAVIS,DEPT NEUROL,DAVIS,CA 95616. UNIV CALIF DAVIS,DEPT INTERNAL MED,DAVIS,CA 95616. NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. VET AFFAIRS MED CTR,BIOCHEM LAB,MARTINEZ,CA 94553. NR 23 TC 34 Z9 35 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 18 PY 1992 VL 31 IS 6 BP 1892 EP 1896 DI 10.1021/bi00121a044 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE194 UT WOS:A1992HE19400044 PM 1737042 ER PT J AU LOVINGER, DM HARRISON, NL LAMBERT, NA AF LOVINGER, DM HARRISON, NL LAMBERT, NA TI THE ACTIONS OF 3-AMINOPROPANEPHOSPHINIC ACID AT GABA(B) RECEPTORS IN RAT HIPPOCAMPUS SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE GABA(B) RECEPTORS (PRESYNAPTIC); GABA(B) RECEPTORS (POSTSYNAPTIC); PATCH SLICE RECORDING; EXCITATORY TRANSMISSION; INHIBITORY TRANSMISSION ID CA3 PYRAMIDAL CELLS; B RECEPTORS; BRAIN SLICES; BACLOFEN; NEURONS; POTENT; 2-HYDROXY-SACLOFEN; TRANSMISSION; SYNAPSES; INVITRO AB The actions of 3-aminopropanephosphinic acid (APPA) were examined using whole-cell patch-clamp recording in rat hippocampal slice. In recordings from neurons in subfield CA1 of slices from young (2-4 weeks) and adult (> 2 month) rats, APPA (0.5-50-mu-M) produced membrane hyperpolarization and outward current under voltage-clamp. APPA also inhibited excitatory postsynaptic potentials with an IC50 of 2.3-mu-M, and reduced inhibitory postsynaptic potentials at concentrations from 0.1 to 1-mu-M. The hyperpolarizing and synaptic depressant effects of APPA were reduced by 2-OH-saclofen an antagonist at the B-type receptor for the neurotransmitter gamma-aminobutyric acid (GABA). In this preparation APPA exhibited potencies similar to those previously reported for the GABA(B) receptor agonist baclofen. APPA was much less effective in inhibiting synaptic transmission measured using field potential recordings. The observations made with whole-cell patch-clamp recording indicate that in hippocampus APPA acts as a potent agonist at presynaptic GABA(B) receptors associated with both excitatory and inhibitory synapses, and also activates postsynaptic GABA(B) receptors. C1 UNIV CHICAGO,SCH MED,DEPT ANESTHESIA & CRIT CARE,CHICAGO,IL 60637. NORTHEASTERN OHIO UNIV,COLL MED,DEPT NEUROBIOL,ROOTSTOWN,OH 44272. NIAAA,ROCKVILLE,MD 20852. NIAAA,LPPS,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852. NR 24 TC 14 Z9 14 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD FEB 18 PY 1992 VL 211 IS 3 BP 337 EP 341 DI 10.1016/0014-2999(92)90390-P PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HG829 UT WOS:A1992HG82900008 PM 1319911 ER PT J AU BRENNEMAN, DE ARIMURA, A AF BRENNEMAN, DE ARIMURA, A TI PACAP PREVENTS NEURONAL CELL-DEATH ASSOCIATED WITH THE ENVELOPE PROTEIN FROM THE HUMAN-IMMUNODEFICIENCY-VIRUS SO REGULATORY PEPTIDES LA English DT Meeting Abstract C1 NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,DEPT MED,BELLE CHASSE,LA 70037. NR 2 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-0115 J9 REGUL PEPTIDES JI Regul. Pept. PD FEB 18 PY 1992 VL 37 IS 3 BP 321 EP 321 DI 10.1016/0167-0115(92)90636-9 PG 1 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA HE326 UT WOS:A1992HE32600024 ER PT J AU HEINDEL, JJ POWELL, CJ PASCHALL, CS ARIMURA, A CULLER, MD AF HEINDEL, JJ POWELL, CJ PASCHALL, CS ARIMURA, A CULLER, MD TI PACAP STIMULATES CAMP ACCUMULATION AND SECRETORY FUNCTION IN CULTURED RAT SERTOLI CELLS SO REGULATORY PEPTIDES LA English DT Meeting Abstract C1 NIEHS,DEV & REP TOX GRP,RES TRIANGLE PK,NC 27709. NIEHS,MOLEC & INT NEURO LAB,RES TRIANGLE PK,NC 27709. TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LAB,MOLEC NEUROENDOCRINOL & DIABET LAB,BELLE CHASSE,LA. NR 2 TC 2 Z9 2 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-0115 J9 REGUL PEPTIDES JI Regul. Pept. PD FEB 18 PY 1992 VL 37 IS 3 BP 333 EP 333 DI 10.1016/0167-0115(92)90645-B PG 1 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA HE326 UT WOS:A1992HE32600033 ER PT J AU CULLER, MD AF CULLER, MD TI PACAP ENHANCES BASAL AND LHRH-STIMULATED GONADOTROPIN-SECRETION SO REGULATORY PEPTIDES LA English DT Meeting Abstract C1 NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,REPROD PHYSIOL SECT,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-0115 J9 REGUL PEPTIDES JI Regul. Pept. PD FEB 18 PY 1992 VL 37 IS 3 BP 334 EP 334 DI 10.1016/0167-0115(92)90646-C PG 1 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA HE326 UT WOS:A1992HE32600034 ER PT J AU RUBIN, JR SWAMINATHAN, P SUNDARALINGAM, M AF RUBIN, JR SWAMINATHAN, P SUNDARALINGAM, M TI STRUCTURE OF THE ANTIMALARIAL DRUG PRIMAQUINE DIPHOSPHATE SO ACTA CRYSTALLOGRAPHICA SECTION C-CRYSTAL STRUCTURE COMMUNICATIONS LA English DT Article ID CRYSTAL AB 8-(4-Amino-1-methylbutylamino)-6-methoxyquinoline bis(dihydrogenphosphate), C15H23N3O2+.2(H2PO4)-, M(r) = 455.35, triclinic, P1BAR, Z = 2, a = 7.389 (6), b = 8.862 (4), c = 16.055 (10) angstrom, alpha = 97.57 (2), beta = 100.21 (3), gamma = 77.01 (2)-degrees, V = 1003.6 (5) angstrom 3, D(m) = 1.495 (by flotation), D(x) = 1.507 g cm-3, lambda(Cu K-alpha) = 1.5418 angstrom, mu(Cu K-alpha) = 24.48 cm-1, F(000) = 480, room temperature, R = 0.068 for 3448 observed reflections. The above working cell is related to the reduced cell with angles alpha = 82.43, beta = 79.79 and gamma = 77.01-degrees by the transformation (-1 0 0/0 - 1 0/0 0 1). Primaquine diphosphate was crystallized in the dicationic form with protonation on the quinoline ring nitrogen atom and on the terminal amino group. One dihydrogenphosphate anion is chelated by the quinoline ring and the butylamino side chain. The other dihydrogenphosphate anion is hydrogen bonded to the terminal amino group. The C(14) atom is nearly in the plane of the quinoline ring with a C(9)-C(8)-N(13)-C(14) torsion angle of 169-degrees. The butyl-diamino side chain is kinked by rotation about the C(14)-C(16) bond with a N(13)-C(14)-C(16)-C(17) torsion angle of -59-degrees. The C(15) methyl substituent is inline with the rest of the butyl chain. The terminal amino group N(19) is hydrogen bonded to three symmetry-related phosphate groups while N(1) and N(13) are 'chelated' to a fourth phosphate group. C1 UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706. RP RUBIN, JR (reprint author), NCI,FREDERICK CANC RES & DEV FACIL,BASIC RES PROGRAM,ABL,POB B,FREDERICK,MD 21701, USA. FU NCI NIH HHS [N01-CO1-74101]; NIGMS NIH HHS [GM-17378] NR 12 TC 2 Z9 2 U1 0 U2 2 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0108-2701 J9 ACTA CRYSTALLOGR C JI Acta Crystallogr. Sect. C-Cryst. Struct. Commun. PD FEB 15 PY 1992 VL 48 BP 379 EP 382 DI 10.1107/S0108270191008831 PN 2 PG 4 WC Chemistry, Multidisciplinary; Crystallography SC Chemistry; Crystallography GA HH428 UT WOS:A1992HH42800040 PM 1627276 ER PT J AU ROFF, CF GOLDIN, E COMLY, ME BLANCHETTEMACKIE, J COONEY, A BRADY, RO PENTCHEV, PG AF ROFF, CF GOLDIN, E COMLY, ME BLANCHETTEMACKIE, J COONEY, A BRADY, RO PENTCHEV, PG TI NIEMANN-PICK TYPE-C DISEASE - DEFICIENT INTRACELLULAR-TRANSPORT OF EXOGENOUSLY DERIVED CHOLESTEROL SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article; Proceedings Paper CT 3RD INTERNATIONAL CONF ON THE NEURONAL CEROID LIPOFUSCINOSES ( BATTEN DISEASE ) CY MAY 30-JUN 01, 1990 CL INDIANAPOLIS, IN SP MARCH DIMES BIRTH DEFECTS FDN, NINCDS, CHILDRENS BRAIN DIS FDN, MERRELL DOW RES LAB, LILLY RES LABS, INDIANAPOLIS UNIV SCH MED DE CHOLESTEROL LIPIDOSIS; INTRACELLULAR CHOLESTEROL TRANSPORT; HYDROPHOBIC AMINES ID LOW-DENSITY LIPOPROTEIN; LYSOSOMAL STORAGE DISORDER; CULTURED FIBROBLASTS; ESTERIFICATION; METABOLISM; ACCUMULATION; PATHWAY; MOUSE; CELLS; MICE AB NPC disease is an autosomal recessive neurovisceral storage disorder. A pleiotropic array of secondary enzymatic and storage abnormalities has in the past obscured a cohesive understanding of the underlying metabolic basis of this disorder. Recent findings, reviewed in this report, demonstrate that NPC disease is a cholesterol lipidosis resulting from defective intracellular cholesterol transport. The sequence of cellular events characteristic of NPC is 1) deficient intracellular transport of exogenously derived cholesterol resulting in retarded induction of cellular cholesterol homeostatic regulation; 2) accumulation of cholesterol in lysosomes; and 3) secondary cellular effects. Retarded esterification of exogenous cholesterol and accumulation of unesterified cholesterol in lysosomes is tightly coupled to the primary defect and serves as the basis for biochemical diagnosis of NPC. C1 NIDDKD,BETHESDA,MD. RP ROFF, CF (reprint author), NINCDS,DEV & METAB NEUROL BRANCH,BLDG 10,ROOM 3D-12,BETHESDA,MD 20892, USA. NR 28 TC 38 Z9 38 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD FEB 15 PY 1992 VL 42 IS 4 BP 593 EP 598 DI 10.1002/ajmg.1320420433 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA HF152 UT WOS:A1992HF15200032 PM 1609841 ER PT J AU BODINE, DM ORLIC, D BIRKETT, NC SEIDEL, NE ZSEBO, KM AF BODINE, DM ORLIC, D BIRKETT, NC SEIDEL, NE ZSEBO, KM TI STEM-CELL FACTOR INCREASES COLONY-FORMING UNIT-SPLEEN NUMBER INVITRO IN SYNERGY WITH INTERLEUKIN-6, AND INVIVO IN SL/SL(D) MICE AS A SINGLE FACTOR SO BLOOD LA English DT Article ID C-KIT RECEPTOR; TYROSINE KINASE RECEPTOR; GROWTH-FACTOR; W-LOCUS; PROTO-ONCOGENE; SI-LOCUS; MOUSE; LIGAND; IDENTIFICATION; PURIFICATION C1 NEW YORK MED COLL,VALHALLA,NY 10595. AMGEN CORP,THOUSAND OAKS,CA. RP BODINE, DM (reprint author), NHLBI,CLIN HEMATOL BRANCH,BLDG 10,RM 7C 103,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 34 TC 89 Z9 90 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 15 PY 1992 VL 79 IS 4 BP 913 EP 919 PG 7 WC Hematology SC Hematology GA HD660 UT WOS:A1992HD66000011 PM 1371079 ER PT J AU ROTTEM, M BARBIERI, S KINET, JP METCALFE, DD AF ROTTEM, M BARBIERI, S KINET, JP METCALFE, DD TI KINETICS OF THE APPEARANCE OF FC-EPSILON-RI-BEARING CELLS IN INTERLEUKIN-3-DEPENDENT MOUSE BONE-MARROW CULTURES - CORRELATION WITH HISTAMINE CONTENT AND MAST-CELL MATURATION SO BLOOD LA English DT Article ID IMMUNOGLOBULIN-E; IGE; DIFFERENTIATION; RECEPTORS; AFFINITY; BINDING; MICE; IDENTIFICATION; SYSTEM C1 NIAID,BIOL RESOURCES BRANCH,FLOW CYTOMETRY SECT,BETHESDA,MD 20892. NIAID,MOLEC ALLERGY & IMMUNOL SECT,BETHESDA,MD 20892. RP ROTTEM, M (reprint author), NIAID,CLIN INVEST LAB,MAST CELL PHYSIOL SECT,BLDG 10,ROOM 11C-210,BETHESDA,MD 20892, USA. NR 29 TC 49 Z9 49 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 15 PY 1992 VL 79 IS 4 BP 972 EP 980 PG 9 WC Hematology SC Hematology GA HD660 UT WOS:A1992HD66000020 PM 1531309 ER PT J AU MICKISCH, GH AKSENTIJEVICH, I SCHOENLEIN, PV GOLDSTEIN, LJ GALSKI, H STAHLE, C SACHS, DH PASTAN, I GOTTESMAN, MM AF MICKISCH, GH AKSENTIJEVICH, I SCHOENLEIN, PV GOLDSTEIN, LJ GALSKI, H STAHLE, C SACHS, DH PASTAN, I GOTTESMAN, MM TI TRANSPLANTATION OF BONE-MARROW CELLS FROM TRANSGENIC MICE EXPRESSING THE HUMAN MDR1 GENE RESULTS IN LONG-TERM PROTECTION AGAINST THE MYELOSUPPRESSIVE EFFECT OF CHEMOTHERAPY IN MICE SO BLOOD LA English DT Article ID MULTIDRUG-RESISTANCE GENE; COLONY-STIMULATING FACTOR; PRODUCT P-GLYCOPROTEIN; FULL-LENGTH CDNA; STEM-CELLS; AUTO-TRANSPLANTATION; TISSUES; DNA; LOCALIZATION; SEQUENCES C1 NCI,MOLEC BIOL LAB,BLDG 37,ROOM 1B22,BETHESDA,MD 20892. NCI,DCBDC,CELL BIOL LAB,BETHESDA,MD 20892. NCI,IMMUNOL BRANCH,BETHESDA,MD 20892. NR 34 TC 78 Z9 80 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 15 PY 1992 VL 79 IS 4 BP 1087 EP 1093 PG 7 WC Hematology SC Hematology GA HD660 UT WOS:A1992HD66000036 PM 1737094 ER PT J AU KONDOH, N SCHWEINFEST, CW HENDERSON, KW PAPAS, TS AF KONDOH, N SCHWEINFEST, CW HENDERSON, KW PAPAS, TS TI DIFFERENTIAL EXPRESSION OF S19 RIBOSOMAL-PROTEIN, LAMININ-BINDING PROTEIN, AND HUMAN LYMPHOCYTE ANTIGEN CLASS-I MESSENGER-RNAS ASSOCIATED WITH COLON-CARCINOMA PROGRESSION AND DIFFERENTIATION SO CANCER RESEARCH LA English DT Article ID CANCER CELL-LINE; HLA CLASS-I; COLORECTAL-CANCER; CDNA CLONES; SEQUENCES; METASTASIS; TUMOR; INITIATION; GENE; SUPPRESSION AB Three complementary DNA encoding S19 ribosomal protein (S19), laminin-binding protein (LBP), and HLA class I (HLA-I) genes were isolated from a colon tumor-enriched subtraction library. To evaluate this mRNA expression, surgically removed colon tumors as well as matched normal tissue and human colon carcinoma cell lines showing various differentiation states, anchorage dependence, and proliferation states were examined by Northern blot analysis. The mRNA level of S19 mRNA (0.6 kilobase) was higher in primary colon carcinoma tissue than in matched normal colon tissue in 5 of 6 cases. In 2 of 4 cases, the expression of LBP mRNA (1.2 kilobases) was higher in carcinoma than in normal tissue. In 12 human colon cell lines, the level of LBP mRNA was higher in poorly differentiated cells. On the other hand, HLA-I mRNA (1.7 kilobases) was higher in well-differentiated cells. Although the S19 mRNA was expressed in both well- and poorly differentiated cells, a concomitant increase with tumor progression was observed in two pairs of cell lines derived from the same patients (SW480 and SW620; COLO201 and COLO205). Anchorage dependence of butyrate-treated HT29 colon carcinoma cells was correlated with lower levels of S19 and LBP mRNAs and higher levels of HLA-I mRNA expression compared with untreated cells. While the expression of S19 and LBP mRNAs was not changed due to cell growth states, HLA-1 mRNA levels were found to be low in proliferating HT29 cells but highly induced in contact-inhibited cells. In summary, therefore, high expression of S19 and LBP combined with low expression of HLA-I were well correlated with colon carcinoma cells of higher malignant potential. RP KONDOH, N (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,BLDG 469,ROOM 205,FREDERICK,MD 21702, USA. NR 44 TC 107 Z9 109 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1992 VL 52 IS 4 BP 791 EP 796 PG 6 WC Oncology SC Oncology GA HD163 UT WOS:A1992HD16300007 PM 1339304 ER PT J AU DUNN, JA FALETTO, MB KASPER, SJ GURTOO, HL AF DUNN, JA FALETTO, MB KASPER, SJ GURTOO, HL TI AFLATOXIN-TRANSFORMED C3H/10T1/2 CELLS OVEREXPRESS PROTEIN-KINASE-C AND HAVE AN ALTERED RESPONSE TO PHORBOL ESTER TREATMENTS SO CANCER RESEARCH LA English DT Article ID ONCOGENE-INDUCED TRANSFORMATION; MOUSE EMBRYO FIBROBLASTS; C3H 10T1/2 CELLS; TUMOR PROMOTERS; C3H-10T1/2 CELLS; INTERCELLULAR COMMUNICATION; POSTCONFLUENCE INHIBITION; CHEMICAL ONCOGENESIS; SIGNAL TRANSDUCTION; MURINE FIBROBLASTS AB The aflatoxin B1-transformed C3H/10T1/2 (10T1/2) cell line 7SA has disordered growth in culture and is tumorigenic in syngeneic mice. Chronic exposure (14 days) of 10T1/2 and 7SA cells to phorbol 12,13-dibutyrate ( PDBu) increased the saturation density of 10T1/2 cells but dramatically slowed the entry of 7SA cells into the log phase of growth without affecting their final saturation density. Similar PDBu treatment of low-density cultures dramatically decreased the size of 7SA colonies. Both cell lines bound [H-3]PDBu in a specific and saturable manner. Analysis of this binding yielded linear Scatchard plots for both cell lines with distinctly different K(d) values (10.7 nM for 10T1/2 versus 54.5 nM for 7SA). The total amount of [H-3]PDBu bound was 2-fold greater in the 7SA cells versus the 10T1/2 cell line. Both cell lines released arachidonic acid following a 2-h exposure to PDBu; however, the magnitude of the response of the 7SA cells was only one-half that of the 10T1/2 cells. Western blot analysis of protein kinase C (PKC) using specific anti-PKC antibodies revealed a greater total amount of PKC(alpha) in the 7SA cells relative to an equal number of 10T1/2 cells. No immunoreactive PKC(alpha) was found in either cell line 16 h after exposure to 600 nM PDBu; however, PKC(alpha) returned to control levels in both cell lines 24 h after removal of the phorbol ester. These results suggest that an overexpression of PKC(alpha) may play a role in the altered biological properties of aflatoxin-transformed 10T1/2 cells. C1 ROSWELL PK CANC INST,GRACE CANC DRUG CTR,BUFFALO,NY 14263. EASTMAN KODAK CO,LIFE SCI RES LAB,ROCHESTER,NY 14650. NIEHS,BIOCHEM RISK ANAL LAB,RES TRIANGLE PK,NC 27709. NR 45 TC 11 Z9 11 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1992 VL 52 IS 4 BP 990 EP 996 PG 7 WC Oncology SC Oncology GA HD163 UT WOS:A1992HD16300036 PM 1737362 ER PT J AU GUTIERREZ, MI BHATIA, K SIWARSKI, D WOLFF, L MAGRATH, IT MUSHINSKI, JF HUPPI, K AF GUTIERREZ, MI BHATIA, K SIWARSKI, D WOLFF, L MAGRATH, IT MUSHINSKI, JF HUPPI, K TI INFREQUENT P53 MUTATION IN MOUSE-TUMORS WITH DEREGULATED MYC SO CANCER RESEARCH LA English DT Note ID BALB/C MICE; PRISTANE; GENE AB An invariant genetic lesion in mouse plasmacytomas is deregulated expression of c-myc as a consequence of chromosomal translocation. However, retroviral and transgenic studies suggest that additional genetic lesions may contribute to the genesis of plasmacytomas. The p53 tumor suppressor gene is a likely contributor to this genetic lesion, since there is a high incidence of p53 mutation in Burkitt's lymphomas and B-ALL (L3), both of which contain translocations involving c-myc analogous to those in plasmacytomas. In addition, p53 has been shown to be a transcriptional modulator of c-myc expression. In a survey of 27 mouse plasmacytomas by single-strand conformation polymorphism, we identified a single mutation (3.7% incidence), suggesting that p53 lesions are not frequent contributors to plasmacytomagenesis. A similar study of macrophage-monocyte tumors generated by a c-myc-containing retrovirus also indicates a lack of p53 involvement in deregulated c-myc expression. These results suggest that the specific maturation stage of transformed B-lymphocytes, independent of c-myc deregulation, may be the critical factor which determines the involvement of mutant p53. C1 NCI,GENET LAB,MOLEC GENET SECT,BLDG 37,ROOM 2B-21,BETHESDA,MD 20892. NCI,PEDIAT BRANCH,BETHESDA,MD 20892. NR 24 TC 28 Z9 28 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1992 VL 52 IS 4 BP 1032 EP 1035 PG 4 WC Oncology SC Oncology GA HD163 UT WOS:A1992HD16300042 PM 1737333 ER PT J AU FUJIMOTO, Y HAMPTON, LL LUO, LD WIRTH, PJ THORGEIRSSON, SS AF FUJIMOTO, Y HAMPTON, LL LUO, LD WIRTH, PJ THORGEIRSSON, SS TI LOW-FREQUENCY OF P53 GENE MUTATION IN TUMORS INDUCED BY AFLATOXIN-B1 IN NONHUMAN-PRIMATES SO CANCER RESEARCH LA English DT Note ID HEPATOCELLULAR-CARCINOMA AB Aflatoxin B1 has been suggested as a causative agent for a G to T mutation at codon 249 in the p53 gene in human hepatocellular carcinomas from southern Africa and Qidong in China. To test this hypothesis, nine tumors induced by aflatoxin B1 in nonhuman primates were analyzed for mutations in the p53 gene. These included four hepatocellular carcinomas, two cholangiocarcinomas, a spindle cell carcinoma of the bile duct, a hemangioendothelial sarcoma of the liver, and an osteogenic sarcoma of the tibia. None of the tumors showed changes at the third position of codon 249 by cleavage analysis of the HaeIII enzyme site at codon 249. A point mutation was identified in one hepatocellular carcinoma at the second position of codon 175 (G to T transversion) by sequencing analysis of the four conserved domains (II to V) in the p53 gene. These data suggest that mutations in the p53 gene are not necessary in aflatoxin B1 induced hepatocarcinogenesis in nonhuman primates. The occurrence of mutation in codon 249 of the p53 gene in selective samples of human hepatocellular cancers may indicate involvement of environmental carcinogens other than aflatoxin B1 or that hepatitis B virus-related hepatitis is a prerequisite for aflatoxin B1 induction of G to T transversion in codon 249. C1 NCI,EXPTL CARCINOGENESIS LAB,BLDG 37,ROOM 3C28,BETHESDA,MD 20892. NR 23 TC 72 Z9 76 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1992 VL 52 IS 4 BP 1044 EP 1046 PG 3 WC Oncology SC Oncology GA HD163 UT WOS:A1992HD16300045 PM 1310637 ER PT J AU ZAMUDIO, F SAAVEDRA, R MARTIN, BM GURROLABRIONES, G HERION, P POSSANI, LD AF ZAMUDIO, F SAAVEDRA, R MARTIN, BM GURROLABRIONES, G HERION, P POSSANI, LD TI AMINO-ACID-SEQUENCE AND IMMUNOLOGICAL CHARACTERIZATION WITH MONOCLONAL-ANTIBODIES OF 2 TOXINS FROM THE VENOM OF THE SCORPION CENTRUROIDES-NOXIUS HOFFMANN SO EUROPEAN JOURNAL OF BIOCHEMISTRY LA English DT Article ID ANDROCTONUS-AUSTRALIS-HECTOR; LIMPIDUS-TECOMANUS HOFFMANN; SODIUM-CHANNEL; 3-DIMENSIONAL STRUCTURE; BRAIN SYNAPTOSOMES; TITYUS-SERRULATUS; RECEPTOR-SITES; K+ CHANNELS; NEUROTOXINS; PROTEIN AB Two toxins, which we propose to call toxins 2 and 3, were purified to homogeneity from the venom of the scorpion Centruroides noxius Hoffmann. The full primary structures of both peptides (66 amino acid residues each) was determined. Sequence comparison indicates that the two new toxins display 79% identity and present a high similarity to previously characterized Centruroides toxins, the most similar toxins being Centruroides suffusus toxin 2 and Centruroides limpidus tecomanus toxin 1. Six monoclonal antibodies (mAb) directed against purified fraction II-9.2 (which contains toxins 2 and 3) were isolated in order to carry out the immunochemical characterization of these toxins. mAb BCF2, BCF3, BCF7 and BCF9 reacted only with toxin 2, whereas BCF1 and BCF8 reacted with both toxins 2 and 3 with the same affinity. Simultaneous binding of mAb pairs to the toxin and cross-reactivity of the venoms of different scorpions with the mAb were examined. The results of these experiments showed that the mAb define four different epitopes (A-D). Epitope A (BCF8) is topographically unrelated to epitopes B (BCF2 and BCF7), C (BCF3) and D (BCF9) but the latter three appear to be more closely related or in close proximity to each other. Epitope A was found in all Centruroides venoms tested as well as on four different purified toxins of C. noxius, and thus seems to correspond to a highly conserved structure. Based on the cross-reactivity of their venoms with the mAb, Centruroides species could be classified in the following order: Centruroides elegans, Centruroides suffusus suffusus = Centruroides infamatus infamatus, Centruroides limpidus tecomanus, Centruroides limpidus limpidus, and Centruroides limpidus acatlanensis, according to increasing immunochemical relatedness of their toxins to those of Centruroides noxius. All six mAb inhibited the binding of toxin 2 to rat brain synaptosomal membranes, but only mAb BCF2, which belongs to the IgG2a subclass, displayed a clear neutralizing activity in vivo. C1 UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,DEPT BIOQUIM,APARTADO POSTAL 510-3,CUERNAVACA 62271,MEXICO. NATL AUTONOMOUS UNIV MEXICO,INST INVEST BIOMED,DEPT INMUNOL,MEXICO CITY 04510,DF,MEXICO. NIMH,CLIN NEUROSCI BRANCH,MOLEC NEUROGENET SECT,BETHESDA,MD 20892. RI Possani, Lourival/J-2397-2013 NR 50 TC 61 Z9 64 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-2956 J9 EUR J BIOCHEM JI Eur. J. Biochem. PD FEB 15 PY 1992 VL 204 IS 1 BP 281 EP 292 DI 10.1111/j.1432-1033.1992.tb16635.x PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE276 UT WOS:A1992HE27600037 PM 1371253 ER PT J AU MAURIZI, MR AF MAURIZI, MR TI PROTEASES AND PROTEIN-DEGRADATION IN ESCHERICHIA-COLI SO EXPERIENTIA LA English DT Review DE ATP-DEPENDENT; DEGRADATION; PROTEASE; LON; CLP ID ATP-DEPENDENT PROTEASE; HEAT-SHOCK PROTEINS; PROLIPOPROTEIN SIGNAL PEPTIDASE; OUTER-MEMBRANE PROTEASE; IN-VIVO DEGRADATION; LON GENE-PRODUCT; ION-GENE; SERINE PROTEASE; MULTICATALYTIC PROTEINASE; INTRACELLULAR PROTEIN AB In E. coli, protein degradation plays important roles in regulating the levels of specific proteins and in eliminating damaged or abnormal proteins. E. coli possess a very large number of proteolytic enzymes distributed in the cytoplasm, the inner membrane, and the periplasm, but, with few exceptions, the physiological functions of these proteases are not known. More than 90% of the protein degradation occurring in the cytoplasm is energy-dependent, but the activities of most E. coli proteases in vitro are not energy-dependent. Two ATP-dependent proteases, Lon and Clp, are responsible for 70-80% of the energy-dependent degradation of proteins in vivo. In vitro studies with Lon and Clp indicate that both proteases directly interact with substrates for degradation. ATP functions as an allosteric effector promoting an active conformation of the proteases, and ATP hydrolysis is required for rapid catalytic turnover of peptide bond cleavage in proteins. Lon and Clp show virtually no homology at the amino acid level, and thus it appears that at least two families of ATP-dependent proteases have evolved independently. RP MAURIZI, MR (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 190 TC 268 Z9 270 U1 1 U2 17 PU BIRKHAUSER VERLAG AG PI BASEL PA PO BOX 133 KLOSTERBERG 23, CH-4010 BASEL, SWITZERLAND SN 0014-4754 J9 EXPERIENTIA JI Experientia PD FEB 15 PY 1992 VL 48 IS 2 BP 178 EP 201 DI 10.1007/BF01923511 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HH175 UT WOS:A1992HH17500007 PM 1740190 ER PT J AU HEIDARAN, MA YU, JC JENSEN, RA PIERCE, JH AARONSON, SA AF HEIDARAN, MA YU, JC JENSEN, RA PIERCE, JH AARONSON, SA TI A DELETION IN THE EXTRACELLULAR DOMAIN OF THE ALPHA-PLATELET-DERIVED GROWTH-FACTOR (PDGF) RECEPTOR DIFFERENTIALLY IMPAIRS PDGF-AA AND PDGF-BB BINDING AFFINITIES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID EGF-RECEPTOR; SIGNAL TRANSDUCTION; LIGAND-BINDING; CDNA CLONING; CELLS; DIMERIZATION; ESTABLISHES; SPECIFICITY; EXPRESSION; DEFINE AB 32D cells transfected with the human alpha-platelet-derived. growth factor receptor (alpha-PDGFR) bind PDGF-AA, -AB, and -BB isoforms with high affinity, and the binding of each can be efficiently competed by all three isoforms. In an effort to develop better understanding of spatial relationships of binding sites for PDGF-AA and -BB, we constructed an alpha-PDGFR mutant which deleted amino acids 150-189 within its extracellular domain. This mutant showed a marked decrease in high affinity binding sites for PDGF-AA without comparable alteration in affinity for PDGF-BB. These findings imply that the high affinity binding sites for PDGF-AA and PDGF-BB in the alpha-PDGFR extracellular domain are not structurally coincident. C1 NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,RM 1E24,BETHESDA,MD 20892. NR 27 TC 24 Z9 24 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 2884 EP 2887 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400013 PM 1310677 ER PT J AU SZWEDA, LI STADTMAN, ER AF SZWEDA, LI STADTMAN, ER TI IRON-CATALYZED OXIDATIVE MODIFICATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE FROM LEUCONOSTOC-MESENTEROIDES - STRUCTURAL AND FUNCTIONAL-CHANGES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MIXED-FUNCTION OXIDATION; GLUTAMINE-SYNTHETASE; OXIDIZED PROTEINS; ESSENTIAL LYSINE; SEQUENCE; RESIDUES; PEPTIDE; SITE AB As a variety of eukaryotic cells age, the specific activity of glucose-6-phosphate dehydrogenase (Glu-6-PDH) declines as much as 50%. Because of the central role of this enzyme in metabolism, it is important to define factors responsible for this loss in enzyme activity. We report that Glu-6-PDH from Leuconostoc mesenteroides is rapidly inactivated by micromolar concentrations of Fe2+ and H2O2. Inactivation correlated with the formation of one carbonyl functionality/enzyme subunit, indicating that inactivation is the result of site-specific oxidative modification. Our results suggest that Fe2+ binds to the glucose 6-phosphate binding site and that interaction of the enzyme-bound Fe2+ with H2O2 leads to the oxidative modification of amino acids essential for enzyme activity. Partially inactivated enzyme remained predominantly in the dimeric form, and no change in the apparent affinity of the remaining active subunits for substrate was observed. Partial inactivation did, however, lead to a decrease in the thermal stability of the remaining activity. This decrease in thermal stability could be largely overcome by the addition of glucose 6-phosphate. Thus, although exposure to H2O2 and Fe2+ results in the irreversible inactivation of Glu-6-PDH, the resulting modification is selective, leads to the formation of heterodimers of both active and inactive subunits, and does not appear to cause large scale structural changes. Our results demonstrate the inherent susceptibility of Glu-6-PDH from L. mesenteroides to modification by an oxidation system known to exist in vivo. An assessment of the physiological significance of Fe2+-catalyzed oxidation of Glu-6-PDH awaits extension of these studies to mammalian sources known to accumulate less active or inactive forms of the enzyme as a function of age. RP SZWEDA, LI (reprint author), NHLBI,BIOCHEM LAB,BLDG 3,RM 221,BETHESDA,MD 20892, USA. NR 34 TC 55 Z9 56 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3096 EP 3100 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400044 PM 1737765 ER PT J AU WALKER, MW BOBAK, DA TSAI, SC MOSS, J VAUGHAN, M AF WALKER, MW BOBAK, DA TSAI, SC MOSS, J VAUGHAN, M TI GTP BUT NOT GDP ANALOGS PROMOTE ASSOCIATION OF ADP-RIBOSYLATION FACTORS, 20-KDA PROTEIN ACTIVATORS OF CHOLERA-TOXIN, WITH PHOSPHOLIPIDS AND PC-12 CELL-MEMBRANES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID NUCLEOTIDE-BINDING-PROTEINS; ADENYLATE-CYCLASE; REGULATORY COMPONENT; GUANINE-NUCLEOTIDES; PLASMA-MEMBRANES; MESSENGER-RNA; BOVINE BRAIN; VESICLES; RAS; YEAST AB ADP-ribosylation factors (ARFs) are a family of approximately 20-kDa guanine nucleotide-binding proteins initially identified by their ability to enhance cholera toxin ADP-ribosyltransferase activity in the presence of GTP. ARFs have been purified from both membrane and cytosolic fractions. ARF purified from bovine brain cytosol requires phospholipid plus detergent for high affinity guanine nucleotide binding and for optimal enhancement of cholera toxin ADP-ribosyltransferase activity. The phospholipid requirements, combined with a putative role for ARF in vesicular transport, suggested that the soluble protein might interact reversibly with membranes. A polyclonal antibody against purified bovine ARF (sARF II) was used to detect ARF by immunoblot in membrane and soluble fractions from rat pheochromocytoma (PC-12) cell homogenates. ARF was predominantly cytosolic but increased in membranes during incubation of homogenates with nonhydrolyzable GTP analogues guanosine 5'-O-(3-thiotriphosphate), guanylyl-(beta-gamma-imido)-diphosphate, and guanylyl-(beta-gamma-methylene)-diphosphate, and to a lesser extent, adenosine 5'-O-(3-thiotriphosphate). GTP, GDP, GMP, and ATP were inactive. Cytosolic ARF similarly associated with added phosphatidylserine, phosphatidylinositol, or cardiolipin in GTP-gamma-S-dependent fashion. ARF binding to phosphatidylserine was reversible and coincident with stimulation of cholera toxin-catalyzed ADP-ribosylation. These observations may reflect a mechanism by which ARF could cycle between soluble and membrane compartments in vivo. RP WALKER, MW (reprint author), NHLBI,CELLULAR METAB LAB,BLDG 10,RM 5N307,900 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 48 TC 52 Z9 52 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3230 EP 3235 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400065 PM 1737779 ER PT J AU RITTER, JK CHEN, F SHEEN, YY TRAN, HM KIMURA, S YEATMAN, MT OWENS, IS AF RITTER, JK CHEN, F SHEEN, YY TRAN, HM KIMURA, S YEATMAN, MT OWENS, IS TI A NOVEL COMPLEX LOCUS UGT1 ENCODES HUMAN BILIRUBIN, PHENOL, AND OTHER UDP-GLUCURONOSYLTRANSFERASE ISOZYMES WITH IDENTICAL CARBOXYL TERMINI SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CLONING; GENE; EXPRESSION; REGION; CELLS; CDNA AB Two human liver UDP-glucuronosyltransferase (transferase) cDNAs, HUG-Br1 and HUG-Br2, were previously isolated (Ritter, J. K., Crawford, J. M., and Owens, I. S. (1991) J. Biol. Chem. 266, 1043-1047), and each was shown to encode a bilirubin transferase isozyme which catalyzes the formation of all physiological conjugates of bilirubin IX-alpha following expression in COS-1 cells. Sequence data showed that the cDNAs contained identical 3' ends (1469 base pairs in length) to each other and to that of the human phenol trans rase cDNA, HLUG P1 (Harding, D., Fournel-Gigleux, S., Jackson, M. R., and Burchell, B. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 8381-8385). Here we report that the two corresponding bilirubin transferases and the phenol transferase are encoded by a novel locus, UGT1, which is also predicted to encode three other bilirubin transferase-like isozymes all having identical carboxyl termini. The transcriptional arrangement utilizes six nested promoter elements, each of which is positioned upstream of a unique exon 1. Each exon 1 encodes the NH2-terminal domain (286 amino acids) and confers the substrate specificity of the isoform. The 3' end of the locus contains 4 common exons which encode the identical carboxyl termini (246 amino acids). It is predicted that six nested primary transcripts are synthesized and that each exon 1 is differentially spliced to the 4 common exons to produce six unique, mature mRNAs. Although the gene organization is present as a single copy, it provides the flexibility of independent regulation of each isoform which is known to occur in the case of bilirubin and phenol transferase activities. With an understanding of the gene structure, lethal, as well as the nonlethal defects, associated with bilirubin transferase activity can now be determined. C1 NCI,MOLEC CARCINOGENESIS LAB,BLDG 10,RM 95 242,BETHESDA,MD 20892. NICHHD,HUMAN GENET BRANCH,BETHESDA,MD 20892. NR 15 TC 419 Z9 429 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3257 EP 3261 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400069 PM 1339448 ER PT J AU TIFFT, CJ PROIA, RL CAMERINIOTERO, RD AF TIFFT, CJ PROIA, RL CAMERINIOTERO, RD TI THE FOLDING AND CELL-SURFACE EXPRESSION OF CD4 REQUIRES GLYCOSYLATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; N-LINKED OLIGOSACCHARIDE; CHAIN BINDING-PROTEIN; INTRACELLULAR-TRANSPORT; LYMPHOCYTES-T; HIV; HEMAGGLUTININ; DOMAIN; SITES; GLYCOPROTEIN AB Human CD4, a monomeric T cell surface glycoprotein, is required for T helper cell activation and is also the receptor for the human immunodeficiency virus. There have been conflicting reports as to whether glycosylation of CD4 is required for its cell surface expression. To clarify the effect of glycosylation on surface expression, folding, and intracellular sorting of CD4, we generated a series of mutant cDNAs in which one, the other, or both glycosylation recognition sites were eliminated. Using in vitro transcription and translation we confirmed that both potential glycosylation sites of CD4 were utilized. Transient expression of the mutants in HeLa cells demonstrated that glycosylation at either site was necessary and sufficient for cell surface expression. Finally, we showed that unglycosylated CD4 produced in HeLa cells was incorrectly folded and retained intracellularly, probably in the endoplasmic reticulum. RP TIFFT, CJ (reprint author), NIDDKD, GENET & BIOCHEM BRANCH, BLDG 10, RM 9D 15, BETHESDA, MD 20892 USA. RI Proia, Richard/A-7908-2012 NR 38 TC 37 Z9 37 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3268 EP 3273 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400071 PM 1737783 ER PT J AU BRODY, LC MITCHELL, GA OBIE, C MICHAUD, J STEEL, G FONTAINE, G ROBERT, MF SIPILA, I KAISERKUPFER, M VALLE, D AF BRODY, LC MITCHELL, GA OBIE, C MICHAUD, J STEEL, G FONTAINE, G ROBERT, MF SIPILA, I KAISERKUPFER, M VALLE, D TI ORNITHINE DELTA-AMINOTRANSFERASE MUTATIONS IN GYRATE ATROPHY - ALLELIC HETEROGENEITY AND FUNCTIONAL CONSEQUENCES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID POLYMERASE CHAIN-REACTION; NUCLEOTIDE-SEQUENCE; NONSENSE MUTATIONS; STRUCTURAL GENE; MOLECULAR-BASIS; MESSENGER-RNA; OAT LOCUS; DNA; DEFICIENCY; RETINA AB Ornithine delta-aminotransferase is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the reversible interconversion of ornithine and alpha-ketoglutarate to glutamate semialdehyde and glutamate. Inherited deficiency of ornithine delta-aminotransferase results in ornithine accumulation and a characteristic chorioretinal degeneration, gyrate atrophy of the choroid and retina. We have surveyed the ornithine delta-aminotransferase genes of gyrate atrophy patients for mutations. Using a variety of techniques, we discovered and molecularly characterized 21 newly recognized ornithine delta-aminotransferase alleles. We determined the consequences of these and three previously described mutations on ornithine delta-aminotransferase mRNA, antigen, and enzyme activity in cultured fibroblasts. The majority (20/24) of these alleles produce normal amounts of normally sized ornithine delta-aminotransferase mRNA. By contrast, only 2/24 had normal amounts of ornithine delta-aminotransferase antigen. Reproducing these mutations by site-directed mutagenesis and expressing the mutant ornithine delta-aminotransferase in Chinese hamster ovary cells confirms that several of these mutations inactivate ornithine delta-aminotransferase and cause gyrate atrophy in these patients. C1 JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST,GENET LAB,PCTB 802,725 N WOLFE ST,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,BALTIMORE,MD 21205. HOP ST JUSTINE,SERV GENET MED,MONTREAL H3T 1C5,QUEBEC,CANADA. CHILDRENS HOSP HELSINKI,DEPT PEDIAT,HELSINKI,FINLAND. NEI,BETHESDA,MD 20892. FU NEI NIH HHS [EY02948] NR 45 TC 74 Z9 75 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3302 EP 3307 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400076 PM 1737786 ER PT J AU SARTOR, O MORIUCHI, R SAMESHIMA, JH SEVERINO, M GUTKIND, JS ROBBINS, KC AF SARTOR, O MORIUCHI, R SAMESHIMA, JH SEVERINO, M GUTKIND, JS ROBBINS, KC TI DIVERSE BIOLOGIC PROPERTIES IMPARTED BY THE C-FGR PROTOONCOGENE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSFORMING GENE-PRODUCT; NIH 3T3 CELLS; TYROSINE PHOSPHORYLATION; SIGNAL TRANSDUCTION; KINASE-ACTIVITY; CD4 RECEPTOR; VIRUS; ACTIVATION; PP60C-SRC; THROMBIN AB The c-fgr proto-oncogene specifies a nonreceptor protein-tyrosine kinase, p55c-fgr, a member of the src family. In the present study, we have mutagenized c-fgr to mimic alterations found at the 3' end of the v-fgr oncogene and have investigated the biologic effects of normal and mutant p55c-fgr expression. Genes lacking 10 or 13 codons at the 3' end, as well as a gene encoding phenylalanine instead of tyrosine at codon 523, were potent oncogenes when transfected into NIH 3T3 cells. Specific enzymatic activities of the more highly transforming gene products were 3-4-fold greater than that of p55c-fgr. In vivo, the amount of tyrosine phosphorylation of cellular proteins was directly proportional to potency in focus-forming assays. These findings are the first to identify highly transforming mutations of the c-fgr proto-oncogene. The proto-oncogene was also active in transforming assays, demonstrably greater than that of a kinase-deficient mutant. Foci arising in c-fgr-transfected cultures expressed abundant enzyme that was normal by a number of criteria. In addition, growth rates for cells expressing p55c-fgr were restricted, as compared with cells expressing a kinase-deficient protein or cells transformed by proteins with high specific enzymatic activities. Thus, enzymatically active p55c-fgr can simultaneously activate transforming and growth inhibitory pathways. C1 NIDR,CELLULAR DEV & ONCOL LAB,BETHESDA,MD 20892. NR 39 TC 20 Z9 21 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1992 VL 267 IS 5 BP 3460 EP 3465 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD154 UT WOS:A1992HD15400098 PM 1737799 ER PT J AU WATANABEFUKUNAGA, R BRANNAN, CI ITOH, N YONEHARA, S COPELAND, NG JENKINS, NA NAGATA, S AF WATANABEFUKUNAGA, R BRANNAN, CI ITOH, N YONEHARA, S COPELAND, NG JENKINS, NA NAGATA, S TI THE CDNA STRUCTURE, EXPRESSION, AND CHROMOSOMAL ASSIGNMENT OF THE MOUSE FAS ANTIGEN SO JOURNAL OF IMMUNOLOGY LA English DT Article ID COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; DNA FRAGMENTATION; CELL-DEATH; MONOCLONAL-ANTIBODY; INTERFERON-GAMMA; GENE; APOPTOSIS; INDUCTION; LINES AB The cell surface Fas antigen is a membrane-associated polypeptide which can mediate apoptosis. cDNA clones encoding the Fas antigen were isolated from a cDNA library constructed with mRNA from the mouse macrophage cell line BAM3. The nucleotide sequence and the deduced amino acid sequence of the mouse Fas antigen were 58.5 and 49.3% identical, respectively, to the corresponding sequences of human Fas antigen cDNA. The mouse Fas antigen consists of 306 amino acids with a calculated M(r) of 34,971 and contains a single transmembrane domain which divides the molecule into extracellular and cytoplasmic domains. A 2.1-kb mRNA coding for the Fas antigen was detected in the mouse thymus, heart, liver, and ovary but not in brain and spleen. The expression of the Fas antigen gene in mouse fibroblast L929 and macrophage BAM3 cell lines was significantly induced by treatment with IFN-gamma but not by IFN-alpha/beta. Interspecific backcross analysis indicated that the gene coding for the Fas antigen is in the distal region of mouse chromosome 19. C1 NCI,FREDERICK CANC RES & DEV CTR,BASIC RES PROGRAM,MAMMALIAN GENET LAB,ADV BIOSCI LABS INC,FREDERICK,MD 21702. TOKYO METROPOLITAN INST MED SCI,BUNKYO KU,TOKYO 113,JAPAN. RP WATANABEFUKUNAGA, R (reprint author), OSAKA BIOSCI INST,6-2-4 FURUEDAI,SUITA,OSAKA 565,JAPAN. FU NCI NIH HHS [N01-CO74101] NR 38 TC 787 Z9 804 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1992 VL 148 IS 4 BP 1274 EP 1279 PG 6 WC Immunology SC Immunology GA HD975 UT WOS:A1992HD97500041 PM 1371136 ER PT J AU MASOLIVER, J PORRA, JM WEISS, GH AF MASOLIVER, J PORRA, JM WEISS, GH TI SOLUTIONS OF THE TELEGRAPHERS EQUATION IN THE PRESENCE OF TRAPS SO PHYSICAL REVIEW A LA English DT Article ID RANDOM-WALK; HEAT WAVES; DIFFUSION; MEDIA AB Several problems in the theory of photon migration in a turbid medium suggest the utility of calculating solutions of the telegrapher's equation in the presence of traps. This paper contains two such solutions for the one-dimensional problem, the first being for a semi-infinite line terminated by a trap, and the second being for a finite line terminated by two traps. Because solutions to the telegrapher's equation represent an interpolation between wavelike and diffusive phenomena, they will exhibit discontinuities even in the presence of traps. C1 NIH, BETHESDA, MD 20892 USA. RP UNIV BARCELONA, DEPT FIS FONAMENTAL, E-08027 BARCELONA, SPAIN. RI Masoliver, Jaume/F-7198-2016 OI Masoliver, Jaume/0000-0002-5810-879X NR 16 TC 36 Z9 36 U1 0 U2 0 PU AMER PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 2469-9926 EI 2469-9934 J9 PHYS REV A JI Phys. Rev. A PD FEB 15 PY 1992 VL 45 IS 4 BP 2222 EP 2227 DI 10.1103/PhysRevA.45.2222 PG 6 WC Optics; Physics, Atomic, Molecular & Chemical SC Optics; Physics GA HE481 UT WOS:A1992HE48100016 ER PT J AU MANECKJEE, R MINNA, JD AF MANECKJEE, R MINNA, JD TI NONCONVENTIONAL OPIOID BINDING-SITES MEDIATE GROWTH INHIBITORY EFFECTS OF METHADONE ON HUMAN LUNG-CANCER CELLS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID FREE DEFINED MEDIUM; CLINICAL SPECIMENS; NEURO-BLASTOMA; NERVOUS-TISSUE; LINES; RECEPTORS; SURVIVAL; PEPTIDES; MICE AB Methadone was found to significantly inhibit the in vitro and in vivo growth of human lung cancer cells. The in vitro growth inhibition (occurring at 1-100 nM methadone) was associated with changes in cell morphology and viability detectable within 1 hr and was irreversible after a 24-hr exposure to the drug. These effects of methadone could be reversed in the first 6 hr by naltrexone, actinomycin D, and cycloheximide, suggesting involvement of opioid-like receptors and the requirement for de novo mRNA and protein synthesis. The inhibitory effects of methadone on the growth of lung cancer cells also could be achieved by the less addictive (+) isomer of methadone. Characterization of the methadone binding to lung cancer cell membranes revealed high-affinity (nM), saturable binding sites for (+/-)-[H-3]methadone, which cross-reacted with ligands for kappa, phencyclidine, sigma, but not mu, and delta-opioid receptors, and the binding characteristics appeared to be different from methadone sites present in rat brain. Methadone decreases cAMP levels in lung cancer cells, but the receptors are not coupled to a pertussis toxin-sensitive guanine nucleotide-binding regulatory protein. We conclude that the lung cancer growth inhibitory effects of methadone are significant, occur at low concentrations, and are mediated by a nonconventional type of opioid binding site distinct from methadone receptors found in the brain. C1 UNIV TEXAS,SW MED CTR,SIMMONS CANC CTR,DALLAS,TX 75235. RP MANECKJEE, R (reprint author), NCI,DIV CANC TREATMENT,NAVY MED ONCOL BRANCH,BETHESDA,MD 20889, USA. NR 23 TC 41 Z9 41 U1 0 U2 2 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1992 VL 89 IS 4 BP 1169 EP 1173 DI 10.1073/pnas.89.4.1169 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HE606 UT WOS:A1992HE60600005 PM 1311082 ER PT J AU DRESSLER, GR DOUGLASS, EC AF DRESSLER, GR DOUGLASS, EC TI PAX-2 IS A DNA-BINDING PROTEIN EXPRESSED IN EMBRYONIC KIDNEY AND WILMS-TUMOR SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID DEVELOPING EXCRETORY SYSTEM; LAMININ-A-CHAIN; PAIRED-BOX; RESTRICTED EXPRESSION; HUMAN CHROMOSOME-11; GENE; INDUCTION; LOCUS; MORPHOGENESIS; TRANSCRIPTS AB The murine Pax-2 gene contains a protein coding domain homologous to the Drosophila paired-box, first described in certain developmental control genes of the segmentation type. Polyclonal antibodies recognize two Pax-2 Proteins that are encoded by differentially spliced mRNAs. The Pax-2 proteins can bind a DNA sequence known to interact with the paired domain of a Drosophila protein. By immunocytochemistry, expression of Pax-2 could be localized to the nuclei of condensing mesenchyme cells and their epithelial derivatives in the developing kidney. Expression is abruptly down-regulated as the tubular epithelium differentiates. High levels of Pax-2 expression could also be detected in the epithelial cells of human Wilms tumors. These data suggest that Pax-2 is a transcription factor active during the mesenchyme-to-epithelium transition in early kidney development and in Wilms tumor. C1 ST JUDE CHILDRENS RES HOSP, DEPT HEMATOL ONCOL, MEMPHIS, TN 38105 USA. UNIV TENNESSEE CTR HLTH SCI, DEPT PEDIAT, DIV HEMATOL ONCOL, MEMPHIS, TN 38163 USA. RP DRESSLER, GR (reprint author), NICHHD, MAMMALIAN GENES & DEV LAB, BETHESDA, MD 20892 USA. FU NCI NIH HHS [CA21765, CA23099] NR 40 TC 327 Z9 329 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1992 VL 89 IS 4 BP 1179 EP 1183 DI 10.1073/pnas.89.4.1179 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HE606 UT WOS:A1992HE60600007 PM 1311084 ER PT J AU HAYES, JJ WOLFFE, AP AF HAYES, JJ WOLFFE, AP TI HISTONES H2A/H2B INHIBIT THE INTERACTION OF TRANSCRIPTION FACTOR-IIIA WITH THE XENOPUS-BOREALIS SOMATIC 5S RNA GENE IN A NUCLEOSOME SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE CHROMATIN; TRANSCRIPTION ID POLYMERASE-II; ACTIVE CHROMATIN; CORE PARTICLES; 5S-RNA GENE; DNA; INVITRO; COMPLEX; INITIATION; BINDING; OOCYTES AB A Xenopus borealis somatic 5S RNA gene was assembled with either the complete octamer of histones, (H2A/H2B/H3/H4)2, or the (H3/H4)2 tetramer of histones that comprises the central protein kernel of the nucleosome. Gel-mobility shifts, DNase I protection, and immunoblotting assays demonstrate that the class III transcription factor IIIA (TFIIIA) readily interacts with SS DNA associated with the tetramer but that little or no binding is detected when 5S DNA is associated with the full octamer of histones. Thus, the presence of histones H2A and H2B in the 5S nucleosome significantly inhibits the interaction of TFIIIA with its cognate binding site within the 5S RNA gene. We propose that either the depletion of histones H2A and H2B from preexisting nucleosomes or the staged assembly of chromatin after replication in which a tetramer of histones H3/H4 associates with DNA before histones H2A/H2B will facilitate the binding of transcription factors to their cognate DNA sequences. RP HAYES, JJ (reprint author), NICHHD,MOLEC EMBRYOL LAB,BLDG 6,ROOM 131,BETHESDA,MD 20892, USA. NR 49 TC 112 Z9 112 U1 0 U2 4 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1992 VL 89 IS 4 BP 1229 EP 1233 DI 10.1073/pnas.89.4.1229 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HE606 UT WOS:A1992HE60600017 PM 1741376 ER PT J AU ENK, AH KATZ, SI AF ENK, AH KATZ, SI TI EARLY MOLECULAR EVENTS IN THE INDUCTION-PHASE OF CONTACT SENSITIVITY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID MACROPHAGE INFLAMMATORY PROTEIN-2; LANGERHANS CELLS; FLUORESCEIN ISOTHIOCYANATE; DENDRITIC CELLS; ANTIGEN; IP-10; SKIN; KERATINOCYTES; EXPRESSION AB To assess changes in epidermis-derived cytokine mRNA levels early in the afferent phase of allergic contact sensitivity, total epidermal mRNA was analyzed at various times after painting skin with haptens. We used a sensitive reverse transcriptase-polymerase chain reaction technique to quantitatively compare the regulation patterns of the following mRNAs: class II major histocompatibility complex I-A-alpha, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL) 1-alpha, IL-1-beta, interferon (IFN)-gamma, granulocyte/macrophage colony-stimulating factor, IFN-induced protein 10, and macrophage inflammatory protein 2. Enhanced Langerhans cell-derived IL-1-beta mRNA signals were detected as early as 15 min after skin painting with allergens. TNF-alpha, IFN-gamma, and granulocyte/macrophage colony-stimulating factor mRNAs were found to be upregulated after application of allergens, irritant, and tolerogens, but class II major histocompatibility complex I-A-alpha, IL-1-alpha, IL-1-beta, IFN-induced protein 10, and macrophage inflammatory protein 2 mRNAs were upregulated only after allergen painting. Depletion of specific cell populations demonstrated that Langerhans cells were the primary source of the IL-1-beta and class II major histocompatibility complex I-A-alpha mRNAs, keratinocytes were the primary source of TNF-alpha, IL-1-alpha, IFN-induced protein 10, and macrophage inflammatory protein 2, and infiltrating T lymphocytes were the source of IFN-gamma. Relevance of the molecular findings was demonstrated by the identification of biologically active IL-1-alpha and immunoreactive TNF-alpha in culture supernatants. These studies demonstrate that Langerhans cell-derived and certain keratinocyte-derived cytokine mRNAs are selectively upregulated by allergens in the very early afferent phase of contact sensitivity. RP ENK, AH (reprint author), NCI,DERMATOL BRANCH,BETHESDA,MD 20892, USA. NR 34 TC 537 Z9 548 U1 0 U2 4 PU NATL ACAD PRESS PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1992 VL 89 IS 4 BP 1398 EP 1402 DI 10.1073/pnas.89.4.1398 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HE606 UT WOS:A1992HE60600053 PM 1741395 ER PT J AU DESMET, MD AF DESMET, MD TI DIFFERENTIAL-DIAGNOSIS OF RETINITIS AND CHOROIDITIS IN PATIENTS WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO AMERICAN JOURNAL OF MEDICINE LA English DT Article; Proceedings Paper CT SYMP ON CLINICAL IMPLICATIONS OF HERPESVIRUS INFECTIONS IN PATIENTS WITH AIDS CY APR 06, 1991 CL NEW YORK, NY SP ASTRA PHARM PROD ID PNEUMOCYSTIS-CARINII CHOROIDITIS; OCULAR TOXOPLASMOSIS; AIDS AB Patients with acquired immunodeficiency syndrome (AIDS) are at high risk for developing retinitis. The most common forms of retinitis in such patients are those caused by cytomegalovirus (CMV) and Toxoplasma; however, retinitis or choroiditis can also be caused by other viral, protozoal, bacterial, and fungal agents. Differential diagnosis of these infections is based on a number of factors, including ophthalmoscopic appearance, underlying disease, clinical history, and severity of underlying immunosuppression. Rapid and accurate diagnosis is essential in preserving functional vision, as some forms of retinitis are rapidly progressive and since appropriate treatment varies by diagnosis. RP DESMET, MD (reprint author), NEI,BLDG 10,ROOM 10N202,BETHESDA,MD 20892, USA. NR 18 TC 9 Z9 10 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD FEB 14 PY 1992 VL 92 SU 2A BP S17 EP S21 DI 10.1016/0002-9343(92)90332-6 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA HF374 UT WOS:A1992HF37400005 PM 1310571 ER PT J AU MASUR, H AF MASUR, H TI CLINICAL IMPLICATIONS OF HERPESVIRUS INFECTIONS IN PATIENTS WITH AIDS - INTRODUCTION SO AMERICAN JOURNAL OF MEDICINE LA English DT Editorial Material RP MASUR, H (reprint author), NIH,BLDG 10,ROOM 7D48,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 6 TC 5 Z9 5 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD FEB 14 PY 1992 VL 92 SU 2A BP S1 EP S2 DI 10.1016/0002-9343(92)90328-9 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA HF374 UT WOS:A1992HF37400001 PM 1310568 ER PT J AU POLIS, MA AF POLIS, MA TI DESIGN OF A RANDOMIZED CONTROLLED TRIAL OF FOSCARNET IN PATIENTS WITH CYTOMEGALOVIRUS RETINITIS ASSOCIATED WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME SO AMERICAN JOURNAL OF MEDICINE LA English DT Article; Proceedings Paper CT SYMP ON CLINICAL IMPLICATIONS OF HERPESVIRUS INFECTIONS IN PATIENTS WITH AIDS CY APR 06, 1991 CL NEW YORK, NY SP ASTRA PHARM PROD ID VIRUS RETINITIS AB In a controlled trial of foscarnet in the treatment of cytomegalovirus (CMV) retinitis in patients with the acquired immunodeficiency syndrome (AIDS), patients with non-immediately sight-threatening lesions were randomized to receive immediate treatment with foscarnet or foscarnet treatment delayed until the first signs of retinitis progression. Foscarnet induction therapy was administered at a dosage of 60 mg/kg 3 times/day via 1-hour intravenous infusion for 21 days. Foscarnet maintenance therapy was administered at a dosage of 90 mg/kg/day via 2-hour infusion. Foscarnet was well tolerated and effective in delaying progression of CMV retinitis in these patients. Final analysis of data from this study and data from other studies will help to determine what role foscarnet will have in the treatment of CMV retinitis in patients with AIDS. RP POLIS, MA (reprint author), NIH,BLDG 10,ROOM 7D43,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 15 TC 0 Z9 0 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD FEB 14 PY 1992 VL 92 SU 2A BP S22 EP S25 DI 10.1016/0002-9343(92)90333-7 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA HF374 UT WOS:A1992HF37400006 PM 1310572 ER PT J AU MCPHIE, P SHRAGER, RI AF MCPHIE, P SHRAGER, RI TI AN INVESTIGATION OF THE THERMAL UNFOLDING OF SWINE PEPSINOGEN USING CIRCULAR-DICHROISM SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID PROTEINS; CONFORMATION; TRANSITIONS; DENATURATION; RESOLUTION; SPECTRA; UREA; ACID C1 NIH,DCRT,APPL STUDIES LAB,BETHESDA,MD 20892. RP MCPHIE, P (reprint author), NIDDK,BIOCHEM & METAB LAB,BLDG 10,ROOM 9N-119,BETHESDA,MD 20892, USA. NR 22 TC 18 Z9 18 U1 1 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD FEB 14 PY 1992 VL 293 IS 1 BP 46 EP 53 DI 10.1016/0003-9861(92)90363-2 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HB251 UT WOS:A1992HB25100007 PM 1731638 ER PT J AU MORALES, TI ROBERTS, AB AF MORALES, TI ROBERTS, AB TI THE INTERACTION BETWEEN RETINOIC ACID AND THE TRANSFORMING GROWTH FACTORS-BETA IN CALF ARTICULAR-CARTILAGE ORGAN-CULTURES SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID PROTEOGLYCANS; BIOSYNTHESIS; METABOLISM; LIPOPOLYSACCHARIDES; INVITRO C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. RP MORALES, TI (reprint author), NIDR,BONE RES BRANCH,BETHESDA,MD 20892, USA. NR 21 TC 25 Z9 25 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD FEB 14 PY 1992 VL 293 IS 1 BP 79 EP 84 DI 10.1016/0003-9861(92)90368-7 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HB251 UT WOS:A1992HB25100012 PM 1731642 ER PT J AU AMIN, N PETERKOFSKY, A AF AMIN, N PETERKOFSKY, A TI REQUIREMENT FOR GLY-60 OF ESCHERICHIA-COLI ADENYLYL CYCLASE FOR ATP BINDING AND CATALYTIC ACTIVITY SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID NUCLEOTIDE-SEQUENCE; GENE-PRODUCTS; EXPRESSION RP AMIN, N (reprint author), NHLBI,BIOCHEM GENET LAB,BETHESDA,MD 20892, USA. NR 16 TC 4 Z9 4 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 14 PY 1992 VL 182 IS 3 BP 1218 EP 1225 DI 10.1016/0006-291X(92)91861-J PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HE697 UT WOS:A1992HE69700034 PM 1540166 ER PT J AU ALESSENKO, A KHAN, WA WETSEL, WC HANNUN, YA AF ALESSENKO, A KHAN, WA WETSEL, WC HANNUN, YA TI SELECTIVE CHANGES IN PROTEIN-KINASE-C ISOENZYMES IN RAT-LIVER NUCLEI DURING LIVER-REGENERATION SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID EXPRESSION; FAMILY; BRAIN; CELLS; FORMS C1 DUKE UNIV,MED CTR,DEPT MED & CELL BIOL,DURHAM,NC 27710. ACAD SCI USSR,DEPT CHEM PHYS,MOSCOW V-71,USSR. NIEHS,MOLEC & INTEGRATED NEUROSCI,RES TRIANGLE PK,NC 27709. FU NCI NIH HHS [CA-47741]; NHLBI NIH HHS [HL-43704] NR 24 TC 38 Z9 40 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 14 PY 1992 VL 182 IS 3 BP 1333 EP 1339 DI 10.1016/0006-291X(92)91879-U PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HE697 UT WOS:A1992HE69700052 PM 1540177 ER PT J AU PUMFORD, NR MARTIN, BM HINSON, JA AF PUMFORD, NR MARTIN, BM HINSON, JA TI A METABOLITE OF ACETAMINOPHEN COVALENTLY BINDS TO THE 56-KDA SELENIUM BINDING-PROTEIN SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID SUBCELLULAR LIVER FRACTIONS; 3-(CYSTEIN-S-YL)ACETAMINOPHEN ADDUCTS; IMMUNOCHEMICAL QUANTITATION; TREATED MICE; HEPATOTOXICITY; TOXICITY; SERUM; ASSAY C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. RP PUMFORD, NR (reprint author), UNIV ARKANSAS MED SCI HOSP,DEPT PHARMACOL & TOXICOL,DIV TOXICOL,LITTLE ROCK,AR 72206, USA. NR 22 TC 90 Z9 92 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 14 PY 1992 VL 182 IS 3 BP 1348 EP 1355 DI 10.1016/0006-291X(92)91881-P PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HE697 UT WOS:A1992HE69700054 PM 1540179 ER PT J AU GERSTEN, DM MOODY, D VIEIRA, WD LAW, LW HEARING, VJ AF GERSTEN, DM MOODY, D VIEIRA, WD LAW, LW HEARING, VJ TI PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST THE B700 MURINE MELANOMA ANTIGEN AND THEIR ANTIMETASTATIC PROPERTIES SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE MELANOMA; MONOCLONAL ANTIBODY; ANTIGEN; ANTIMETASTATIC ID B-16 MELANOMA; TUMOR REJECTION; CELLS; MICE; METASTASIS; RECEPTOR; PROTEINS; THERAPY; SERA AB Two unique murine melanoma antigens, termed B700 and B50, have been identified and isolated from several different murine melanoma cell lines. Both antigens can be detected on the cell surface, are actively shed in culture, and are often found in close association intracellularly. In previous studies, the antigen B700, which is related to serum albumin by biochemical and immunological criteria, was shown to function as a melanoma-specific tumor rejection antigen. We have also shown that animals sensitized to irradiated JB/RH melanoma cells produce antibodies which recognize B700 and/or B50, with B700 evoking the stronger humoral response. Animals testing positive by ELISA for antibody production to B700 or B50 were used for preparation of hybridomas and four different murine monoclonal antibodies have been produced whose specificities should facilitate epitope mapping. Clones have been used to generate ascites fluid in nude mice; the antibodies specifically recognize B700 and intact murine melanoma cells, but not B50. Two of these monoclonal antibodies have been administered systemically to C57B1/6 mice bearing 5 day pulmonary metastases of the JB/MS melanoma, and significant inhibition of metastatic growth was observed for both antibodies. C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. RP GERSTEN, DM (reprint author), GEORGETOWN UNIV,MED CTR,DEPT PATHOL,WASHINGTON,DC 20007, USA. NR 31 TC 10 Z9 10 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD FEB 14 PY 1992 VL 1138 IS 2 BP 109 EP 114 DI 10.1016/0925-4439(92)90049-S PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HG738 UT WOS:A1992HG73800004 PM 1540656 ER PT J AU BRADY, LS GOLD, PW HERKENHAM, M LYNN, AB WHITFIELD, HJ AF BRADY, LS GOLD, PW HERKENHAM, M LYNN, AB WHITFIELD, HJ TI THE ANTIDEPRESSANTS FLUOXETINE, IDAZOXAN AND PHENELZINE ALTER CORTICOTROPIN-RELEASING HORMONE AND TYROSINE-HYDROXYLASE MESSENGER-RNA LEVELS IN RAT-BRAIN - THERAPEUTIC IMPLICATIONS SO BRAIN RESEARCH LA English DT Article DE ANTIDEPRESSANT DRUG; CORTICOTROPIN-RELEASING HORMONE; DEPRESSION; GLUCOCORTICOID RECEPTOR; INSITU HYBRIDIZATION; LOCUS COERULEUS; MINERALOCORTICOID RECEPTOR; PARAVENTRICULAR NUCLEUS; TYROSINE HYDROXYLASE ID ATYPICAL DEPRESSION; BIOCHEMICAL MANIFESTATIONS; RECEPTOR; EXPRESSION; IMIPRAMINE; NEUROBIOLOGY; LOCALIZATION; RESPONDERS; SECRETION; SEQUENCE AB Various classes of antidepressant drugs with distinct pharmacologic actions are differentially effective in the treatment of classic melancholic depression - characterized by pathological hyperarousal and atypical depression - associated with lethargy, hypersomnia, and hyperphagia. All antidepressant agents exert their therapeutic efficacy only after prolonged administration. In situ hybridization histochemistry was used to examine in rats the effects of short-term (2 weeks) and long-term (8 weeks) administration of 3 different classes of activating antidepressant drugs which tend to be preferentially effective in treating atypical depressions, on the expression of central nervous system genes thought to be dysregulated in major depression. Daily administration (5 mg/kg, i.p.) of the selective 5-hydroxytryptophan (5-HT) re-uptake inhibitor fluoxetine, the selective alpha-2-adrenergic receptor antagonist idazoxan, and the nonspecific monoamine oxidase A and B inhibitor phenelzine increased tyrosine hydroxylase mRNA levels by 70-150% in the locus coeruleus after 2 weeks of drug and by 71-115% after 8 weeks. The 3 drugs decreased corticotropin-releasing hormone mRNA levels by 30-48% in the paraventricular nucleus of the hypothalamus. The decreases occurred at 8 weeks but not at 2 weeks. No consistent change in steroid hormone receptor mRNA levels was seen in the hippocampus with the 3 drugs, but fluoxetine and idazoxan increased the level of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) mRNA, respectively, after 8 weeks of drug administration. Proopiomelanocortin (POMC) mRNA levels in the anterior pituitary and plasma adrenocorticotropic-hormone (ACTH) levels were not altered after 2 or 8 weeks of drug treatment. Plasma corticosterone levels were decreased by all 3 drugs at the 2-week time point, and corticosterone continued to be reduced in the phenelzine-treated animals after 8 weeks of chronic drug administration. We have previously demonstrated that the prototypic antidepressant imipramine shares with fluoxetine, idazoxan, and phenelzine the property of decreasing the expression of corticotropin-releasing hormone mRNA in the paraventricular nucleus after chronic administration. In contrast, imipramine, which is preferentially effective in treating melancholic depression, produces a significant decrease in tyrosine hydroxylase mRNA levels in the locus coeruleus as opposed to the effect of the other 3 drugs. Thus, our data suggest that the preferential efficacy of activating antidepressants in atypical depression may reflect a capacity to increase the expression of tyrosine hydroxylase mRNA in the locus coeruleus, an effect that could theoretically be utilized in the screening of pharmacologic agents for the treatment of this disorder. Overall, the data suggest that a reduction in the expression of corticotropin-releasing hormone mRNA in the paraventricular nucleus may be one common element relevant to the therapeutic efficacy of antidepressant drugs in the treatment of various forms of major depression. RP BRADY, LS (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,FUNCT NEUROANAT SECT,BLDG 36,BETHESDA,MD 20892, USA. OI Herkenham, Miles/0000-0003-2228-4238 NR 41 TC 205 Z9 210 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 14 PY 1992 VL 572 IS 1-2 BP 117 EP 125 DI 10.1016/0006-8993(92)90459-M PG 9 WC Neurosciences SC Neurosciences & Neurology GA HE988 UT WOS:A1992HE98800017 PM 1351783 ER PT J AU BABILA, T SCHAAD, NC KLEIN, DC AF BABILA, T SCHAAD, NC KLEIN, DC TI RAT PINEAL GS-ALPHA, GI-ALPHA AND GO-ALPHA - RELATIVE ABUNDANCE AND DEVELOPMENT SO BRAIN RESEARCH LA English DT Note DE GS; GI; GO; GTP-BINDING PROTEIN; PINEAL; DEVELOPMENT ID ADENYLATE-CYCLASE SYSTEM; GTP-BINDING PROTEINS; CHOLERA-TOXIN; RECEPTOR; ANTIBODIES; BRAIN AB The adult rat pineal gland contains relatively high concentrations of Gs-alpha, low amounts of both Gi-alpha and Go-alpha, and undetectable levels of GT-alpha. During development the amounts of 45 kDa Gs-alpha and of Gi-alpha remain constant. In contrast, 42 kDa Gs-alpha and Go-alpha are nearly absent at birth and increase in abundance markedly thereafter. GT-alpha is undetectable at any age. It would appear that multiple mechanisms regulate the expression of G-proteins in the pineal gland. C1 NICHHD,DEV NEUROBIOL LAB,NEUROENDOCRINOL SECT,BETHESDA,MD 20892. NR 24 TC 13 Z9 13 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 14 PY 1992 VL 572 IS 1-2 BP 232 EP 235 DI 10.1016/0006-8993(92)90474-N PG 4 WC Neurosciences SC Neurosciences & Neurology GA HE988 UT WOS:A1992HE98800032 PM 1611517 ER PT J AU GESSAIN, A BOERI, E KAZADI, K GARIN, B SALAUN, JJ GALLO, R DETHE, G FRANCHINI, G AF GESSAIN, A BOERI, E KAZADI, K GARIN, B SALAUN, JJ GALLO, R DETHE, G FRANCHINI, G TI IDENTIFICATION OF AN HTLV-I VARIANT IN A ZAIRIAN PATIENT WITH A TSP HAM - NUCLEOTIDIC SEQUENCE OF THE ENVELOPE GENE SO COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES LA French DT Article ID TROPICAL SPASTIC PARAPARESIS; T-CELL LEUKEMIA; VIRUS TYPE-I; MYELOPATHY; ANTIBODIES; CLUSTER; ISOLATE AB The oncoretrovirus HTLV-I is the etiological agent of adult T cell leukemia (ATL) and tropical spastic paraparesis/HLTV-I associated myelopathy (TSP/HAM). In contrast to the human lentiretroviruses, HIV-I and HIV-2, the causative agents of AIDS, HTLV-I is genetically very stable. We report here the molecular characterization of the envelope gene of an HTLV-I variant present in a TSP/HAM patient from Zaire, raising the question of its relevance to disease or the ethnic and/or geographical origin of the patient. C1 UNIV KINSHASA,CTR NATL NEUROPSYCHOPATHOL,KINSHASA,ZAIRE. INST RECH BIOMED,GOMBE KINSHASA,ZAIRE. INST PASTEUR,UNITE EPIDEMIOL VIRUS ONCOGENES,F-75724 PARIS 15,FRANCE. RP GESSAIN, A (reprint author), NCI,TUMOR CELL BIOL LAB,BLDG 3720892,BETHESDA,MD 20892, USA. NR 24 TC 11 Z9 11 U1 0 U2 0 PU JOHN LIBBEY EUROTEXT LTD PI MONTROUGE PA 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE SN 0764-4469 J9 CR ACAD SCI III-VIE JI Comptes Rendus Acad. Sci. Ser. III-Sci. Vie-Life Sci. PD FEB 13 PY 1992 VL 314 IS 4 BP 159 EP 164 PG 6 WC Biology; Multidisciplinary Sciences SC Life Sciences & Biomedicine - Other Topics; Science & Technology - Other Topics GA HH339 UT WOS:A1992HH33900002 PM 1576541 ER PT J AU YEW, N MELLINI, ML VANDEWOUDE, GF AF YEW, N MELLINI, ML VANDEWOUDE, GF TI MEIOTIC INITIATION BY THE MOS PROTEIN IN XENOPUS SO NATURE LA English DT Article ID ONCOGENE PRODUCT; CELL-CYCLE; CYTOPLASMIC FACTOR; OOCYTE MATURATION; KINASE-ACTIVITY; EGGS AB WHEN fully grown Xenopus oocytes are stimulated by progesterone, a period of protein synthesis is necessary for maturation 1. Synthesis of the mos proto-oncogene product, pp39mos, is necessary for the activation of M-phase promoting factor (MPF) in meiosis I (ref. 2). On the basis that mos is translated de novo on hormonal stimulation of Xenopus oocytes 3 and that injecting mos RNA into oocytes induces their maturation 3,4, we have proposed that the mos protein is a candidate initiator of oocyte maturation, needed to trigger the conversion of precursor MPF into its active form 1-3. To determine whether mos is the only protein required for initiating maturation, we have produced a soluble, active recombinant mos protein and injected it into Xenopus oocytes. We report here that in the absence of protein synthesis that mos protein efficiently induces germinal vesicle breakdown and the activation of MPF. The oocytes, however, do not proceed into meiosis II. Thus, the mos protein fulfills the requirements of an initiator protein, but the synthesis of one or more additional proteins may be necessary to complete oocyte maturation. C1 NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP INC,PROT ISOLAT LAB,FREDERICK,MD 21702. RP YEW, N (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP INC,ABL,BASIC RES PROGRAM,POB B,FREDERICK,MD 21702, USA. NR 23 TC 214 Z9 214 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW SN 0028-0836 J9 NATURE JI Nature PD FEB 13 PY 1992 VL 355 IS 6361 BP 649 EP 652 DI 10.1038/355649a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA HD547 UT WOS:A1992HD54700060 PM 1531698 ER PT J AU KUMAR, VD HARRISON, RW ANDREWS, LC WEBER, IT AF KUMAR, VD HARRISON, RW ANDREWS, LC WEBER, IT TI CRYSTAL-STRUCTURE AT 1.5-A RESOLUTION OF D(CGCICICG), AN OCTANUCLEOTIDE CONTAINING INOSINE, AND ITS COMPARISON WITH D(CGCG) AND D(CGCGCG) STRUCTURES SO BIOCHEMISTRY LA English DT Article ID DOUBLE-HELICAL DNA; MOLECULAR-STRUCTURE; CRYSTALLOGRAPHIC REFINEMENT; ATOMIC RESOLUTION; CONFORMATION; RNA; DEOXYINOSINE; D(CPGPCPG); DUPLEX; BASES AB The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space group P6(5)22 with unit cell dimensions of a = b = 31.0 angstrom and c = 43.7 angstrom, and X-ray diffraction data have been collected to 1.5-angstrom resolution. Precession photographs and the self-Patterson function indicate that 12 base pairs of Z-conformation DNA stack along the c-axis, and the double helices pack in a hexagonal array similar to that seen in other crystals of Z-DNA. The structure has been solved by both Patterson deconvolution and molecular replacement methods and refined in space group P6(5) to an R factor of 0.225 using 2503 unique reflections greater than 3.0-sigma(F). Comparison of the molecules within the hexagonal lattice with highly refined crystal structures of other Z-DNA reveals only minor conformational differences, most notably in the pucker of the deoxyribose of the purine residues. The DNA has multiple occupancy of C:I and C:G base pairs, and C:I base pairs adopt a conformation similar to that of C:G base pairs. C1 NCI, FREDERICK CANC RES & DEV CTR,ADV BIOSCI LABS, BASIC RES PROGRAM,MACROMOLEC STRUCT LAB, FREDERICK, MD 21702 USA. RP KUMAR, VD (reprint author), THOMAS JEFFERSON UNIV, DEPT PHARMACOL, BLUEMLE LIFE SCI BLDG, 233 S 10TH ST, PHILADELPHIA, PA 19107 USA. FU NCI NIH HHS [N01-CO-74101] NR 31 TC 27 Z9 27 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 11 PY 1992 VL 31 IS 5 BP 1541 EP 1550 DI 10.1021/bi00120a035 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HD157 UT WOS:A1992HD15700035 PM 1737011 ER PT J AU LIAW, YC GAO, YG MARQUEZ, VE WANG, AHJ AF LIAW, YC GAO, YG MARQUEZ, VE WANG, AHJ TI MOLECULAR-STRUCTURES OF 2 NEW ANTI-HIV NUCLEOSIDE ANALOGS - 9-(2,3-DIDEOXY-2-FLUORO-BETA-D-THREO-PENTOFURANOSYL)ADENINE AND 9-(2,3-DIDEOXY-2-FLUORO-BETA-D-THREO-PENTOFURANOSYL)HYPOXANTHINE SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; REVERSE-TRANSCRIPTASE; CRYSTAL-STRUCTURES; AIDS VIRUS; CONFORMATION; INHIBITOR; 2',3'-DIDEOXYCYTIDINE; 2',3'-DIDEOXYNUCLEOSIDES; INVITRO; AGENTS AB The x-ray crystal structures of two new anti-HIV compounds, 9-(2,3-dideoxy-2-fluoro-beta-D-threo-pentofuranosyl) adenine (2'-F-dd-araA) and 9-(2,3-dideoxy-2-fluoro-beta-D-threo-pentofuranosyl) hypoxanthine (2'-F-dd-aral), have been determined at two temperatures. Both crystals are in the space group P2(1)2(1)2(1), and their structures were solved by direct methods. Least-squares refinement produced final R-factors of 0.027 for the 2'-F-dd-araA structure and of 0.044 for the 2'-F-dd-aral structure, respectively. The latter structure contains a two-fold disordered conformation of the sugar moiety. All three conformers (one for 2'-F-dd-araA and two for 2'-F-dd-aral) adopt an anti(chi)CN glycosyl torsion angle. The sugar in the 2'-F-dd-araA structure has a C2-endo pucker conformation, whereas the sugar in the 2'-F-dd-aral structure has a mixture of C2'-endo and C3'-endo pucker conformations. When the sugar adopts the C2'-endo conformation, the torsion angle about the C4'-C5' bond is in a trans-gauche+ conformation. In contrast, when the sugar adopts the C3'-endo conformation, the torsion angle about the C4'-C5' bond is in a gauche+-gauche- conformation. The C2'-F bond distance is 1.406(3) angstrom, similar to that found in other aliphatic C-F bonds. The results suggest that the 2'-fluoro-2',3'-dideoxyarabinosyl nucleosides do not have a strong preference for either C2-endo or C3'-endo sugar pucker. C1 UNIV ILLINOIS,DEPT PHYSIOL & BIOPHYS,URBANA,IL 61801. NCI,DCT,DTP,MED CHEM LAB,BETHESDA,MD 20892. NR 30 TC 13 Z9 13 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 11 PY 1992 VL 20 IS 3 BP 459 EP 465 DI 10.1093/nar/20.3.459 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE570 UT WOS:A1992HE57000012 PM 1741280 ER PT J AU BUONANNO, A APONE, L MORASSO, MI BEERS, R BRENNER, HR EFTIMIE, R AF BUONANNO, A APONE, L MORASSO, MI BEERS, R BRENNER, HR EFTIMIE, R TI THE MYOD FAMILY OF MYOGENIC FACTORS IS REGULATED BY ELECTRICAL-ACTIVITY - ISOLATION AND CHARACTERIZATION OF A MOUSE MYF-5 CDNA SO NUCLEIC ACIDS RESEARCH LA English DT Article ID RECEPTOR ALPHA-SUBUNIT; CREATINE-KINASE GENE; MESSENGER-RNA LEVELS; CELL-PROLIFERATION; DENERVATED MUSCLE; SKELETAL-MUSCLE; SOLEUS MUSCLE; BETA-SUBUNIT; EXPRESSION; SEQUENCE AB A full-length cDNA coding for a homolog of the human Myf-5 was isolated from a BC3H-1 mouse library and characterized. The clone codes for a protein of 255 amino acids that is 89%, 88% and 68% identical to the human, bovine and Xenopus myf-5, respectively. The mouse Myf-5 cDNA (mmyf-5), as well as sequences coding for MyoD, myogenin and Mrf-4, were used to probe Northern blots to analyze the effects of innervation on the expression of the MyoD family of myogenic factors. Mouse myf-5, MyoD and myogenin mRNAs levels were found to decline in hind limb muscles of mice between embryonic day 15 (E15) and the first postnatal week, a period that coincides with innervation. In contrast, Mrf-4 transcripts increase during this period and reach steady-state levels by 1-week after birth. To distinguish if the changes in myogenic factor expression are due to a developmental program or to innervation, mRNA levels were analyzed at different times after muscle denervation. Mmyf-5 transcripts begin to accumulate 2 days post-denervation; after 1 week levels are 7-fold higher than in innervated muscle. Mrf-4, MyoD and myogenin transcripts begin to accumulate as soon as 8h after denervation, and attain levels that are 8-, 15- and 40-fold higher than found in innervated skeletal muscle, respectively. The accumulation of these three mRNAs precedes the increase of nicotinic acetylcholine receptor alpha-subunit transcripts, a gene that is transcriptionally regulated by MyoD-related factors in vitro. Using extracellular electrodes to directly stimulate in situ the soleus muscle of rats, we found that 'electrical activity' per se, in absence of the nerve, represses the increases of myogenic factor mRNAs associated with denervation. C1 UNIV BASEL,INST PHYSIOL,CH-4051 BASEL,SWITZERLAND. RP BUONANNO, A (reprint author), NICHHD,DEV NEUROBIOL LAB,UNIT MOLEC NEUROBIOL,BLDG 36,ROOM 2A 21,BETHESDA,MD 20892, USA. NR 54 TC 114 Z9 115 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 11 PY 1992 VL 20 IS 3 BP 539 EP 544 DI 10.1093/nar/20.3.539 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HE570 UT WOS:A1992HE57000024 PM 1741288 ER PT J AU ESHHAR, N HUNTER, C WENTHOLD, RJ WADA, K AF ESHHAR, N HUNTER, C WENTHOLD, RJ WADA, K TI STRUCTURAL CHARACTERIZATION AND EXPRESSION OF A BRAIN SPECIFIC GENE ENCODING CHICK KAINATE BINDING-PROTEIN SO FEBS LETTERS LA English DT Article DE GLUTAMATE RECEPTOR; KAINATE; GENE STRUCTURE; PROMOTER; TRANSCRIPTION; INSITU HYBRIDIZATION ID KAINIC ACID RECEPTOR; GLUTAMATE RECEPTOR; LOCALIZATION; DNA; AFFINITY AB The gene encoding chick kainate-binding protein (c-KBP), a member of the non-NMDA ionotropic glutamate receptor family has been isolated and characterized. The c-KBP gene is at least 13 kilobases long and contains 11 exons interrupted by 10 introns. Primer extension and RNase protection studies identified a major transcription initiation site located 117 bases upstream from the initiation methionine codon ATG. Consensus TATA and CCAAT sequences were detected in the putative promoter region. The structure of the c-KBP gene is strinkingly different from that of other members of neurotransmitter-gated ion-channels (cloned at present) although the topology of c-KBP consists of four membrane-spanning domains, a structural characteristic of ionotropic receptor subunits. The c-KBP gene was found to be expressed at high levels in chick cerebellar Bergmann glia and at extremely low levels in the forebrain. The limited expression of the c-KBP gene raises important questions concerning the mechanisms governing the regulation of c-KBP gene transcription. RP ESHHAR, N (reprint author), NIDCD,NEUROCHEM LAB,BLDG 36,ROOM 5D-08,BETHESDA,MD 20892, USA. NR 25 TC 14 Z9 14 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD FEB 10 PY 1992 VL 297 IS 3 BP 257 EP 262 DI 10.1016/0014-5793(92)80551-Q PG 6 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA HE215 UT WOS:A1992HE21500014 PM 1312013 ER PT J AU JACOBSON, MA BESCH, CL CHILD, C HAFNER, R MATTS, JP MUTH, K WENTWORTH, DN DEYTON, L AF JACOBSON, MA BESCH, CL CHILD, C HAFNER, R MATTS, JP MUTH, K WENTWORTH, DN DEYTON, L TI TOXICITY OF CLINDAMYCIN AS PROPHYLAXIS FOR AIDS-ASSOCIATED TOXOPLASMIC ENCEPHALITIS SO LANCET LA English DT Note ID NERVOUS-SYSTEM TOXOPLASMOSIS AB A double-blind, placebo-controlled trial was set up to compare clindamycin and pyrimethamine as prophylaxis for toxoplasmic encephalitis (TE) in HIV-infected patients at risk of the disorder. Interim analysis showed that clindamycin-treated patients were 4.4 (95% confidence interval 1.3-15.2) times more likely to experience an adverse effect that necessitated withdrawal of the study drug than those who received placebo. Diarrhoea and rash were reported in 16 (31%) and 11 (21%), respectively, of 52 patients treated with clindamycin (300 mg twice daily) compared with 2 (6%; p = 0.06) and none (p = 0.01) of the 32 placebo-treated patients. The clindamycin arm of the trial was prematurely terminated, although recruitment to the pyrimethamine arm continues. C1 NIAID,DIV AIDS,6003 EXECUT BLVD,BETHESDA,MD 20892. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143. SAN FRANCISCO GEN HOSP,MED SERV,SAN FRANCISCO,CA 94110. SAN FRANCISCO COMMUNITY CONSORTIUM AIDS,SAN FRANCISCO,CA. TULANE UNIV,NEW ORLEANS,LA 70118. UNIV MINNESOTA,DIV BIOSTAT,MINNEAPOLIS,MN 55455. ROW SCI,BETHESDA,MD. NR 8 TC 42 Z9 42 U1 0 U2 1 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD FEB 8 PY 1992 VL 339 IS 8789 BP 333 EP 334 DI 10.1016/0140-6736(92)91649-S PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA HC771 UT WOS:A1992HC77100006 PM 1346413 ER PT J AU PEOPLES, RW WEIGHT, FF AF PEOPLES, RW WEIGHT, FF TI ETHANOL INHIBITION OF N-METHYL-D-ASPARTATE-ACTIVATED ION CURRENT IN RAT HIPPOCAMPAL-NEURONS IS NOT COMPETITIVE WITH GLYCINE SO BRAIN RESEARCH LA English DT Note DE N-METHYL-D-ASPARTATE; ETHANOL; GLYCINE; HIPPOCAMPUS; RAT ID NMDA RECEPTOR; RELEASE; SLICES; BRAIN; CELLS AB The interaction of ethanol with glycine at the N-methyl-D-aspartate (NMDA)-activated ion channel was investigated in voltage-clamped rat cultured hippocampal neurons. As shown previously, glycine increased, and ethanol inhibited, the NMDA-activated current in these cells. Concentration-response data for glycine (0.1-100-mu-M) indicate that the inhibition of NMDA-activated current by ethanol does not involve a competitive interaction with glycine. Thus, ethanol appears to inhibit NMDA-activated current at a locus different from the glycine modulatory site. RP PEOPLES, RW (reprint author), NIAAA,PHYSIOL & PHARMACOL STUDIES LAB,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852, USA. NR 14 TC 80 Z9 80 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 7 PY 1992 VL 571 IS 2 BP 342 EP 344 DI 10.1016/0006-8993(92)90674-X PG 3 WC Neurosciences SC Neurosciences & Neurology GA HE699 UT WOS:A1992HE69900022 PM 1377089 ER PT J AU TAMKUN, JW DEURING, R SCOTT, MP KISSINGER, M PATTATUCCI, AM KAUFMAN, TC KENNISON, JA AF TAMKUN, JW DEURING, R SCOTT, MP KISSINGER, M PATTATUCCI, AM KAUFMAN, TC KENNISON, JA TI BRAHMA - A REGULATOR OF DROSOPHILA HOMEOTIC GENES STRUCTURALLY RELATED TO THE YEAST TRANSCRIPTIONAL ACTIVATOR SNF2 SW12 SO CELL LA English DT Article ID DOSAGE-DEPENDENT MODIFIERS; BITHORAX COMPLEX GENES; ANTENNAPEDIA GENE; SACCHAROMYCES-CEREVISIAE; MOLECULAR ANALYSIS; SPATIAL REGULATION; TRITHORAX GENES; SELECTOR GENE; FUSHI-TARAZU; 2 PROMOTERS AB The brahma (brm) gene is required for the activation of multiple homeotic genes in Drosophila. Loss-of-function brm mutations suppress mutations in Polycomb, a repressor of homeotic genes, and cause developmental defects similar to those arising from insufficient expression of the homeotic genes of the Antennapedia and Bithorax complexes. The brm gene encodes a 1638 residue protein that is similar to SNF2/SWI2, a protein involved in transcriptional activation in yeast, suggesting possible models for the role of brm in the transcriptional activation of homeotic genes. In addition, both brm and SNF2 contain a 77 amino acid motif that is found in other Drosophila, yeast, and human regulatory proteins and may be characteristic of a new family of regulatory proteins. C1 UNIV COLORADO,DEPT MOLEC CELLULAR & DEV BIOL,BOULDER,CO 80309. INDIANA UNIV,HOWARD HUGHES MED INST,BLOOMINGTON,IN 47405. INDIANA UNIV,GENET PROGRAM,BLOOMINGTON,IN 47405. UNIV ALBERTA,DEPT GENET,EDMONTON T6G 2E9,ALBERTA,CANADA. NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. RP TAMKUN, JW (reprint author), UNIV CALIF SANTA CRUZ,DEPT BIOL,SANTA CRUZ,CA 95064, USA. NR 67 TC 686 Z9 696 U1 0 U2 14 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD FEB 7 PY 1992 VL 68 IS 3 BP 561 EP 572 DI 10.1016/0092-8674(92)90191-E PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HD398 UT WOS:A1992HD39800015 PM 1346755 ER PT J AU DEVER, TE FENG, L WEK, RC CIGAN, AM DONAHUE, TF HINNEBUSCH, AG AF DEVER, TE FENG, L WEK, RC CIGAN, AM DONAHUE, TF HINNEBUSCH, AG TI PHOSPHORYLATION OF INITIATION FACTOR-2-ALPHA BY PROTEIN-KINASE GCN2 MEDIATES GENE-SPECIFIC TRANSLATIONAL CONTROL OF GCN4 IN YEAST SO CELL LA English DT Article ID AMINO-ACID BIOSYNTHESIS; DOUBLE-STRANDED-RNA; POLYPEPTIDE-CHAIN INITIATION; SACCHAROMYCES-CEREVISIAE; ALPHA-SUBUNIT; FACTOR-II; MESSENGER-RNA; FACTOR EIF-2; START-SITE; EXPRESSION AB We show that phosphorylation of the a subunit of eukaryotic translation initiation factor 2 (eIF-2) by the protein kinase GCN2 mediates translational control of the yeast transcriptional activator GCN4. In vitro, GCN2 specifically phosphorylates the alpha-subunit of rabbit or yeast eIF-2. In vivo, phosphorylation of eIF-2-alpha increases in response to amino acid starvation, which is dependent on GCN2. Substitution of Ser-51 with alanine eliminates phosphorylation of eIF-2-alpha by GCN2 in vivo and in vitro and abolishes increased expression of GCN4 and amino acid biosynthetic genes under its control in amino acid-starved cells. The Asp-51 substitution mimics the phosphorylated state and derepresses GCN4 in the absence of GCN2. Thus, an established mechanism for regulating total protein synthesis in mammalian cells mediates gene-specific translational control in yeast. C1 INDIANA UNIV, DEPT BIOL, BLOOMINGTON, IN 47405 USA. RP NICHHD, MOLEC GENET LOWER EUKARYOTES SECT, BETHESDA, MD 20892 USA. FU NIGMS NIH HHS [GM32263] NR 47 TC 460 Z9 469 U1 3 U2 12 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 EI 1097-4172 J9 CELL JI Cell PD FEB 7 PY 1992 VL 68 IS 3 BP 585 EP 596 DI 10.1016/0092-8674(92)90193-G PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HD398 UT WOS:A1992HD39800017 PM 1739968 ER PT J AU JACOBSON, KA VANGALEN, PJM WILLIAMS, M AF JACOBSON, KA VANGALEN, PJM WILLIAMS, M TI ADENOSINE RECEPTORS - PHARMACOLOGY, STRUCTURE-ACTIVITY-RELATIONSHIPS, AND THERAPEUTIC POTENTIAL SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Review ID RAT-BRAIN MEMBRANES; PIG CEREBRAL-CORTEX; BINDING SUBUNIT; HIGH-AFFINITY; A2-ADENOSINE RECEPTORS; PHOTOAFFINITY CROSSLINKING; SPECIES-DIFFERENCES; GLYCOPROTEIN NATURE; STRIATAL MEMBRANES; HUMAN-FIBROBLASTS C1 ABBOTT LABS,DIV NEUROSCI RES PHARMACEUT PROD,D 464,ABBOTT PK,IL 60064. NIDDK,BIOORGAN CHEM LAB,BETHESDA,MD 20892. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20, Z99 DK999999] NR 170 TC 487 Z9 489 U1 3 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD FEB 7 PY 1992 VL 35 IS 3 BP 407 EP 422 DI 10.1021/jm00081a001 PG 16 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA HD266 UT WOS:A1992HD26600001 PM 1738138 ER PT J AU CLEMENS, JD FERRECCIO, C LEVINE, MM HORWITZ, I RAO, MR EDWARDS, KM FRITZELL, B AF CLEMENS, JD FERRECCIO, C LEVINE, MM HORWITZ, I RAO, MR EDWARDS, KM FRITZELL, B TI IMPACT OF HAEMOPHILUS-INFLUENZAE TYPE-B POLYSACCHARIDE-TETANUS PROTEIN CONJUGATE VACCINE ON RESPONSES TO CONCURRENTLY ADMINISTERED DIPHTHERIA-TETANUS-PERTUSSIS VACCINE SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID CAPSULAR POLYSACCHARIDE; SERUM ANTIBODIES; EFFICACY; CHILDREN; INFANTS; DISEASE; ALASKA AB Objective. - To assess whether serum antibody responses to diphtheria-tetanus-pertussis (DTP) vaccine were affected by coadministration of Haemophilus influenzae type b capsular polyribosylribitol phosphate polysaccharide-tetanus protein (PRP-T) conjugate vaccine when given to patients at 2, 4, and 6 months of age. Design. - Randomized, double-blind clinical trial. Setting. - Urban Santiago, Chile. Patients. - Healthy infants assembled from health centers, Two hundred seventy-eight (74%) of 375 eligible infants participated; 222, who complied with the complete protocol, constituted the primary group under analysis. Interventions. - One of three vaccine regimens was given to study participants at 2,4, and 6 months of age, either DTP mixed in the same syringe as PRP-T (group 1); DTP and PRP-T given at separate injection sites (group 2); or DTP without PRP-T (group 3). Primary Outcome Measures. - Titers of serum antidiphtheria toxoid, antitetanus toxoid, and pertussis agglutinin antibodies were measured in blood samples taken from patients 2 months after each dose. Results. - Serum antidiphtheria toxoid and antitetanus toxoid responses showed no important depressions in the patients receiving PRP-T. In contrast, geometric mean titers (GMTs) of pertussis agglutinins, expressed as reciprocal serum dilutions, after both the second and third doses (GMT2, GMT3) were lowest in group 1 (GMT2=89; GMT3=1230), intermediate in group 2 (GMT2 = 123; GMT3 = 1995), and highest in group 3 (GMT2 = 210; GMT3 = 3090; P < .05 for trend group 1 < group 2 < group 3 after each dose). Antipertussis toxin and antipertussis filamentous hemagglutinin antibody titers also were de-pressed in patients who received PRP-T. Follow-up of a subset at 18 months revealed an expected decline of pertussis agglutinin titers to near baseline levels in each group. Conclusions. - Concurrent administration of PRP-T vaccine with DTP vaccine, either in the same syringe or at different sites, interfered with antipertussis responses to a primary series of immunizations. Although the clinical significance of this antagonism is uncertain, these data underscore the caution required in decisions to add new vaccines to existing immunization regimens. C1 NICHHD,DIV EPIDEMIOL STAT & PREVENT RES,BETHESDA,MD 20892. ROBERTO RIO HOSP,DEPT PEDIAT,SANTIAGO,CHILE. UNIV MARYLAND,SCH MED,CTR VACCINE DEV,BALTIMORE,MD 21201. MINIST CHILE,SANTIAGO,CHILE. VANDERBILT UNIV,MED CTR,SCH MED,DEPT PEDIAT,NASHVILLE,TN 37232. NR 28 TC 100 Z9 101 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 5 PY 1992 VL 267 IS 5 BP 673 EP 678 DI 10.1001/jama.267.5.673 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA HB354 UT WOS:A1992HB35400024 PM 1731134 ER PT J AU KELLEY, CA SELLERS, JR GOLDSMITH, PK ADELSTEIN, RS AF KELLEY, CA SELLERS, JR GOLDSMITH, PK ADELSTEIN, RS TI SMOOTH-MUSCLE MYOSIN IS COMPOSED OF HOMODIMERIC HEAVY-CHAINS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID CYTOPLASMIC MYOSINS; MULTIGENE FAMILY; MESSENGER-RNAS; GENE; PROTEINS; INVITRO; CELLS; EXPRESSION; MOVEMENT; CHICKEN AB Vertebrate smooth muscle myosin heavy chains (MHCs) exist as two isoforms with molecular masses of 204 and 200 kDa (MHC204 and MHC200) that are generated from a single gene by alternative splicing of mRNA (Nagai, R., Kuro-o, M., Babij, P., and Periasamy, M. (1989) J. Biol. Chem. 264, 9734-9737). A dimer of two MHCs associated with two pairs of myosin light chains forms a functional myosin molecule. To investigate the isoform composition of the MHCs in native myosin, antibodies specific for MHC204 were generated and used to immunoprecipitate purified bovine aortic smooth muscle myosin from a solution containing equal amounts of each isoform. MHC204 quantitatively removed from this mixture was completely free of MHC200. Immunoprecipitation of the supernatant with an antiserum that recognizes both isoforms equally well revealed that only MHC200 remained. We conclude that only homodimers of MHC204 and MHC200 exist under these conditions. A method is described for the purification of enzymatically active MHC204 and MHC200 homodimers by affinity chromatography of myosin on a protein G-agarose high performance liquid chromatography column containing immobilized MHC204 antibodies. We show, using an in vitro motility assay, that the movement of actin filaments by myosin containing 204-kDa heavy chains (0.435 +/- 0.115-mu-m/s) was not significantly different from that of myosin containing 200-kDa heavy chains (0.361 +/- 0.078-mu-m/s) or from myosin containing equal amounts of each heavy chain isoform (0.347 +/- 0.082-mu-m/s). C1 NIDDKD,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD 20892. RP KELLEY, CA (reprint author), NHLBI,MOLEC CARDIOL LAB,BLDG 10,RM 8N-202,BETHESDA,MD 20892, USA. OI Adelstein, Robert/0000-0002-8683-2144 NR 37 TC 75 Z9 75 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1992 VL 267 IS 4 BP 2127 EP 2130 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB532 UT WOS:A1992HB53200004 PM 1733920 ER PT J AU STRACKE, ML KRUTZSCH, HC UNSWORTH, EJ ARESTAD, A CIOCE, V SCHIFFMANN, E LIOTTA, LA AF STRACKE, ML KRUTZSCH, HC UNSWORTH, EJ ARESTAD, A CIOCE, V SCHIFFMANN, E LIOTTA, LA TI IDENTIFICATION, PURIFICATION, AND PARTIAL SEQUENCE-ANALYSIS OF AUTOTAXIN, A NOVEL MOTILITY-STIMULATING PROTEIN SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-MELANOMA CELLS; RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; CANCER-PATIENT FIBROBLASTS; HUMAN-TUMOR CELLS; GROWTH-FACTOR; SCATTER FACTOR; HAPTOTACTIC MIGRATION; SIGNAL TRANSDUCTION; FACTOR RECEPTOR; CHEMOTAXIS AB Autotaxin (ATX) is a potent human motility-stimulating protein that has been identified in the conditioned medium from A2058 melanoma cells. This protein has been purified to homogeneity utilizing a strategy involving five column steps. Homogeneity of ATX was verified by two-dimensional gel electrophoresis. The molecular size of ATX is 125 kDa, and it has an isoelectric point of 7.7 +/- 0.2. Purified ATX was digested with cyanogen bromide and trypsin, and the resulting ATX peptides were purified by reverse-phase high performance liquid chromatography. Eleven peptides were subjected to amino acid sequence analysis, and 114 residues were identified. The partial amino acid sequences and the amino acid composition obtained for ATX show that it does not exhibit any significant homology to known growth factors or previously described motility factors. At picomolar concentrations, ATX stimulates both random and directed migration of human A2058 melanoma cells. Pretreatment of the melanoma cells with pertussis toxin abolishes the response to purified ATX, indicating that ATX stimulates motility through a receptor acting via a pertussis toxin-sensitive G protein. RP STRACKE, ML (reprint author), NCI,PATHOL LAB,BLDG 10,RM 2A33,BETHESDA,MD 20892, USA. NR 40 TC 382 Z9 390 U1 1 U2 9 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1992 VL 267 IS 4 BP 2524 EP 2529 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB532 UT WOS:A1992HB53200064 PM 1733949 ER PT J AU GEISER, AG BURMESTER, JK WEBBINK, R ROBERTS, AB SPORN, MB AF GEISER, AG BURMESTER, JK WEBBINK, R ROBERTS, AB SPORN, MB TI INHIBITION OF GROWTH BY TRANSFORMING GROWTH-FACTOR-BETA FOLLOWING FUSION OF 2 NONRESPONSIVE HUMAN CARCINOMA CELL-LINES - IMPLICATION OF THE TYPE-II RECEPTOR IN GROWTH INHIBITORY RESPONSES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LIVER EPITHELIAL-CELLS; HUMAN-LUNG FIBROBLASTS; PLASMINOGEN-ACTIVATOR INHIBITOR; MESSENGER-RNA EXPRESSION; TGF-BETA; REVERSIBLE INHIBITION; IV COLLAGENASE; PROLIFERATION; FACTOR-BETA-1; BINDING AB Loss of growth regulation by transforming growth factor-beta (TGF-beta) may be an important step in carcinogenesis. We have used a cell fusion system to show that inhibition of growth by TGF-beta can be restored to carcinoma cell lines that are unresponsive to the inhibitory effects of TGF-beta. In a previous study, the EJ bladder carcinoma line was fused to the SW480 colon adenocarcinoma line and found to produce nontumorigenic hybrid cells along with one hybrid cell clone of low tumorigenicity. Here we show that the capacity of the nontumorigenic hybrid cells to respond to either TGF-beta-1 or TGF-beta-2 has been restored, while the parental or tumorigenic hybrid cells show little or no inhibition of growth following TGF-beta treatment. Crosslinking analyses with labeled TGF-beta-1 demonstrated much higher levels of the type II (85 kDa) receptor in the hybrid cells compared with the parental tumor lines. Both the parental and tumorigenic hybrid cell lines were capable of responding to TGF-beta as evidenced by increased levels of mRNA for fibronectin, type IV collagenase, and plasminogen activator inhibitor after treatment with TGF-beta-1. These results suggest that the type II receptor is necessary for mediating the effects of TGF-beta on inhibition of growth but not on gene activation of the hybrid cells. RP GEISER, AG (reprint author), NCI,CHEMOPREVENT LAB,BLDG 41,RM C629,BETHESDA,MD 20892, USA. NR 48 TC 177 Z9 178 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1992 VL 267 IS 4 BP 2588 EP 2593 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB532 UT WOS:A1992HB53200074 PM 1370826 ER PT J AU FUJIMURA, T WICKNER, RB AF FUJIMURA, T WICKNER, RB TI INTERACTION OF 2 CIS SITES WITH THE RNA REPLICASE OF THE YEAST L-A VIRUS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DOUBLE-STRANDED-RNA; Q-BETA REPLICASE; SACCHAROMYCES-CEREVISIAE; INVITRO; POLYMERASE; PARTICLES; PROTEIN; BINDING; TRANSCRIPTASE; RECOGNITION AB L-A is a 4.6-kilobase double-stranded RNA virus of Saccharomyces cerevisiae. The in vitro L-A replication reaction ((-)-strand synthesis) requires an internal site 400 bases from the 3' end in addition to the 3'-terminal 30 nucleotides of the L-A (+)-single-stranded RNA. Elimination of the internal site reduces the template activity 5-10-fold. Here we investigate how the internal site can stimulate the replication reaction which starts at the 3' end of the template. When these two sites are split into two distinct RNA molecules, the internal site can no longer stimulate replication (no trans-activation). However, establishment of an intermolecular hydrogen bonding between these RNAs restored the replication-enhancing activity of the internal site. This result is consistent with a model wherein L-A's RNA polymerase interacts first with the internal site and then with the 3' end site by either looping or by a local dissociation-reassociation mechanism. These results, however, clearly eliminate anchored tracking and sliding models which require continuity of the RNA molecule between these two cis sites. RP FUJIMURA, T (reprint author), NIDDKD,BIOCHEM PHARMACOL LAB,GENET SIMPLE EUKARYOTES SECT,BLDG 8,RM 207,BETHESDA,MD 20892, USA. RI Fujimura, Tsutomu/K-5807-2014 OI Fujimura, Tsutomu/0000-0002-9457-6769 NR 29 TC 28 Z9 29 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1992 VL 267 IS 4 BP 2708 EP 2713 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB532 UT WOS:A1992HB53200090 PM 1733966 ER PT J AU MORONI, MC WILLINGHAM, MC BEGUINOT, L AF MORONI, MC WILLINGHAM, MC BEGUINOT, L TI EGF-R ANTISENSE RNA BLOCKS EXPRESSION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR AND SUPPRESSES THE TRANSFORMING PHENOTYPE OF A HUMAN CARCINOMA CELL-LINE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MESSENGER-RNA; PROTO-ONCOGENE; FACTOR-ALPHA; OLIGOMER COMPLEMENTARY; INHIBITS PROLIFERATION; TRANSLATION INVIVO; GENE-EXPRESSION; 3T3 CELLS; KB CELLS; INVITRO AB We have used an antisense approach to investigate the role of overexpression of the normal human epidermal growth factor (EGF) receptor in the transformed phenotype of KB cells, which are a tumor derived human cell line. Initial experiments performed in vitro, showed that antisense RNA complementary to the entire coding region (AS-FL) or to parts of the EGF-R mRNA (AS-3', AS-5', and AS-K) effectively blocked translation of EGF-R mRNA. In addition, upon microinjection into KB cells, the in vitro synthesized antisense RNAs were able to inhibit transiently the synthesis of EGF-R. Inhibition was concentration-dependent, both in vitro and in cells, and the most effective constructs were those complementary to the entire coding region (ASFL) or to the 3'-coding end of the mRNA (AS-3'). Transfection of the same EGF-R antisense RNA constructs into the human epidermoid carcinoma KB cell line gave rise to several clones stably expressing elevated levels of antisense RNA and resulting in low residual levels of EGF receptor. The most reduced clones exhibited a totally restored serum-dependent growth and were severely impaired in colony formation and growth in agar. In addition the severity of the phenotype was directly proportional to the residual amount of EGF-R expressed. We conclude that over-expression of normal EGF-R plays a direct primary role in the development of the transformed phenotype of this human cancer cell line. C1 UNIV COPENHAGEN,INST MICROBIOL,DK-1353 COPENHAGEN,DENMARK. NIH,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 54 TC 92 Z9 93 U1 0 U2 5 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1992 VL 267 IS 4 BP 2714 EP 2722 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB532 UT WOS:A1992HB53200091 PM 1733967 ER PT J AU MOORE, TD KORN, EL AF MOORE, TD KORN, EL TI PHASE-II TRIAL DESIGN CONSIDERATIONS FOR SMALL-CELL LUNG-CANCER SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Editorial Material ID PREVIOUSLY UNTREATED PATIENTS; BRONCHOGENIC CARCINOMA; INTERNATIONAL-ASSOCIATION; TENIPOSIDE VM-26; CLINICAL-TRIALS; CHEMOTHERAPY; DRUGS; CISPLATIN; WORKSHOP; PROGRESS C1 NCI,DIV CANC TREATMENT,BIOMETR RES BRANCH,CANC THERAPY EVALUAT PROGRAM,EPN-739,BETHESDA,MD 20892. NCI,CLIN INVEST BRANCH,BETHESDA,MD 20892. NR 41 TC 23 Z9 23 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 5 PY 1992 VL 84 IS 3 BP 150 EP 154 DI 10.1093/jnci/84.3.150 PG 5 WC Oncology SC Oncology GA HG727 UT WOS:A1992HG72700008 PM 1311772 ER PT J AU HENDRIX, MJC SEFTOR, EA CHU, YW SEFTOR, REB NAGLE, RB MCDANIEL, KM LEONG, SPL YOHEM, KH LEIBOVITZ, AM MEYSKENS, FL CONAWAY, DH WELCH, DR LIOTTA, LA STETLERSTEVENSON, W AF HENDRIX, MJC SEFTOR, EA CHU, YW SEFTOR, REB NAGLE, RB MCDANIEL, KM LEONG, SPL YOHEM, KH LEIBOVITZ, AM MEYSKENS, FL CONAWAY, DH WELCH, DR LIOTTA, LA STETLERSTEVENSON, W TI COEXPRESSION OF VIMENTIN AND KERATINS BY HUMAN-MELANOMA TUMOR-CELLS - CORRELATION WITH INVASIVE AND METASTATIC POTENTIAL SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID INTERMEDIATE FILAMENTS; MALIGNANT-MELANOMA; BASEMENT-MEMBRANE; IMMUNOHISTOCHEMICAL SPECTRUM; CYTOKERATIN EXPRESSION; MONOCLONAL-ANTIBODIES; CYTOSKELETAL ELEMENTS; MOUSE EMBRYOGENESIS; MELANOCYTIC TUMORS; IV COLLAGENASE AB Background: Several protein markers, including vimentin, have been used to diagnose human melanoma. Because melanoma often has metastasized by the time of diagnosis, early markers prognostic for metastatic potential need to be identified. Commonly, vimentin is found in mesenchymal cells, and keratins are present in epithelial cells, but recent studies report coexpression of vimentin and keratin(s) in epithelial and nonepithelial neoplasms, including some melanomas. Purpose: Our purpose was to determine whether coexpression of vimentin and keratin(s) is correlated with tumor cell invasion and metastatic behavior. Methods: We evaluated nine human melanoma cell lines expressing vimentin and other markers of aggressive tumor behavior (HMB-45, S-100, HLA-ABC class I and HLA-DR class II histocompatibility antigens, and K8 and K18 keratins). Levels of K8 and K18 keratins were determined in the highly metastatic C8161 cell line, the poorly metastatic A375P line, and the moderately metastatic A375M line. To determine whether the presence of keratin affects migratory ability, we altered the conformational structure of keratin filaments in C8161 cells by transfection with a mutant K18 complementary DNA. We also determined messenger RNA levels of human type IV collagenase, an enzyme marker for invasion and metastasis. Results: In A375P cells, two-dimensional electrophoresis with Coomassie-stained gels, immunoblotting, and immunofluorescence staining showed no detectable levels of K8 or K18. A375M cells showed low levels of K8 and K18 by Western and Northern blotting, with a distinctive fluorescent subpopulation of cells. In comparison, K8 and K18 levels in C8161 cells were high in all cells. Type IV collagenase messenger RNA levels were lowest in A375P cells and highest in C8161 cells, correlating with invasive ability in vitro and metastatic potential in athymic nude mice. The transfectant clones C1070-10 and C1070-14 derived from the C8161 parent line showed dramatic morphological changes, disrupted keratin filaments, and decreased invasive and metastatic potential directly correlated with a reduction in migratory activity. Conclusion: These findings show a correlation between the coexpression of vimentin with K8 and K18 keratins and the invasive and metastatic behavior of three representative human melanoma cell lines. C1 ARIZONA CANC CTR,TUCSON,AZ. UNIV ARIZONA,COLL MED,DEPT PATHOL,TUCSON,AZ 85721. UNIV ARIZONA,COLL MED,DEPT SURG,TUCSON,AZ 85721. UNIV CALIF IRVINE,CTR CANC,DEPT HEMATOL ONCOL,IRVINE,CA 92717. MICHIGAN CANC FDN,DETROIT,MI 48201. PENN STATE UNIV,MILTON S HERSHEY MED CTR,SCH MED,DEPT PATHOL,HERSHEY,PA 17033. NCI,DIV CANC BIOL DIAGNOSIS,PATHOL LAB,BETHESDA,MD 20892. RP HENDRIX, MJC (reprint author), UNIV ARIZONA,COLL MED,DEPT ANAT,TUCSON,AZ 85724, USA. RI Welch, Danny/B-7310-2009; Stetler-Stevenson, William/H-6956-2012 OI Welch, Danny/0000-0002-1951-4947; Stetler-Stevenson, William/0000-0002-5500-5808 FU NCI NIH HHS [CA-54984] NR 71 TC 150 Z9 151 U1 0 U2 4 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 5 PY 1992 VL 84 IS 3 BP 165 EP 174 DI 10.1093/jnci/84.3.165 PG 10 WC Oncology SC Oncology GA HG727 UT WOS:A1992HG72700010 PM 1371813 ER PT J AU MANOS, MM REINGOLD, A SCHIFFMAN, M AF MANOS, MM REINGOLD, A SCHIFFMAN, M TI PREVALENCE OF HUMAN PAPILLOMAVIRUS IN YOUNG-WOMEN - RESPONSE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter C1 UNIV CALIF BERKELEY,EPIDEMIOL PROGRAM,BERKELEY,CA 94720. NCI,DIV CANC ETIOL,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. RP MANOS, MM (reprint author), CETUS CORP,DEPT INFECT DIS,1400 53RD ST,EMERYVILLE,CA 94608, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 5 PY 1992 VL 84 IS 3 BP 202 EP 202 DI 10.1093/jnci/84.3.202-a PG 1 WC Oncology SC Oncology GA HG727 UT WOS:A1992HG72700016 ER PT J AU SMITH, M UNGERLEIDER, RS HOROWITZ, ME SIMON, R AF SMITH, M UNGERLEIDER, RS HOROWITZ, ME SIMON, R TI RETROSPECTIVE REVIEW OF NEOADJUVANT CHEMOTHERAPY FOR OSTEOGENIC-SARCOMA - RESPONSE SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter RP SMITH, M (reprint author), NCI,CANC THERAPY EVALUAT PROGRAM,EXECUT PLAZA N,RM 741,BETHESDA,MD 20892, USA. NR 6 TC 1 Z9 1 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 5 PY 1992 VL 84 IS 3 BP 203 EP 204 DI 10.1093/jnci/84.3.203 PG 2 WC Oncology SC Oncology GA HG727 UT WOS:A1992HG72700018 ER PT J AU GAO, XM CHUANG, DM AF GAO, XM CHUANG, DM TI CARBAMAZEPINE-INDUCED NEUROTOXICITY AND ITS PREVENTION BY NMDA IN CULTURED CEREBELLAR GRANULE CELLS SO NEUROSCIENCE LETTERS LA English DT Article DE CARBAMAZEPINE; NEUROTOXICITY; NMDA; CEREBELLAR GRANULE CELL; VERATRIDINE; LITHIUM; COLCHICINE ID ANTICONVULSANTS; SEIZURES; SYSTEM; RAT AB Long-term neurotoxicity of carbamazepine was studied in cultured cerebellar granule cells. Treatment of cells with carbamazepine for 3 days induced a dose-dependent neurotoxicity detected by a loss of [H-3]ouabain binding to Na+,K+-ATPase, and [H-3]N-methyl scopolamine binding to muscarinic cholinergic receptors as well as by direct morphologic examination. NMDA protected against carbamazepine-induced toxicity and this protection was blocked by 2-amino-5-phosphono-valerate (APV). The neurotoxicity induced by carbamazepine may be involved in the teratogenic and adverse effects of overdose associated with the treatment of manic-depressive illness and seizures. C1 NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,ROOM 3N212,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NR 18 TC 15 Z9 15 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD FEB 3 PY 1992 VL 135 IS 2 BP 159 EP 162 DI 10.1016/0304-3940(92)90426-8 PG 4 WC Neurosciences SC Neurosciences & Neurology GA HE026 UT WOS:A1992HE02600004 PM 1352629 ER PT J AU LUEBKE, JI WEIGHT, FF AGUAYO, LG AF LUEBKE, JI WEIGHT, FF AGUAYO, LG TI LABELING AND RECORDING FROM DISSOCIATED TARGET-SPECIFIC RAT SUPERIOR CERVICAL-GANGLION NEURONS SO NEUROSCIENCE LETTERS LA English DT Article DE PATCH-CLAMP; FLUORESCENT TRACER; NEURON; RETROGRADE-TRANSPORT; FAST BLUE; SUPERIOR CERVICAL GANGLION ID SYMPATHETIC NEURON; SURVIVAL INVITRO; CELLS; MOTONEURONS; CURRENTS; SODIUM AB A population of neurons was retrogradely labelled in the superior cervical ganglia (SCG) of the adult rat following the injection of the fluorescent dye Fast blue into the submandibular salivary glands (SMG). The neurons retained the fluorescent label following dissociation and culture. Electrical and chemosensitive properties of the labelled neurons were studied with the whole-cell patch-clamp technique. C1 NIAAA,ELECTROPHYSIOL SECT,ROCKVILLE,MD 20852. BOSTON UNIV,SCH MED,DEPT ANAT,BOSTON,MA 02118. NR 18 TC 8 Z9 8 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD FEB 3 PY 1992 VL 135 IS 2 BP 210 EP 214 DI 10.1016/0304-3940(92)90438-D PG 5 WC Neurosciences SC Neurosciences & Neurology GA HE026 UT WOS:A1992HE02600016 PM 1378213 ER PT J AU CETRON, MS HOFF, R KAHN, S EISEN, H VANVOORHIS, WC AF CETRON, MS HOFF, R KAHN, S EISEN, H VANVOORHIS, WC TI EVALUATION OF RECOMBINANT TRYPOMASTIGOTE SURFACE-ANTIGENS OF TRYPANOSOMA-CRUZI IN SCREENING SERA FROM A POPULATION IN RURAL NORTHEASTERN BRAZIL ENDEMIC FOR CHAGAS-DISEASE SO ACTA TROPICA LA English DT Article DE CHAGAS DISEASE, DIAGNOSIS; SEROPREVALENCE; RECOMBINANT ANTIGENS; TRYPANOSOMA-CRUZI ID LINKED IMMUNOSORBENT-ASSAY; SERODIAGNOSIS; ANTIBODY; INFECTION; CLONING; CELLS; GENE AB A perfect serologic test for infection with Trypanosoma cruzi does not exist. This study uses recombinant T. cruzi surface proteins in the antibody capture enzyme linked immunoabsorption assay (ELISA); and compares this approach to the more standard immunoflorescence assay (IFA). Three recombinant antigens are studied: F1-160 corresponding to the 160 kDa flagellar associated surface protein of trypomastigotes (the motile form of T. cruzi in mammalian infections); and SA 85-1.1 and 1.2 corresponding to different members of the 85 kDa family of surface proteins expressed by trypomastigotes and amastigotes (the replicative, non-motile form of T. cruzi in mammalian infections). Each recombinant antigen is found to be highly specific (range 86-94%) but relatively insensitive (range 36-52%) when used to screen for antibodies to T. cruzi. Defining seropositivity as reactivity to any of the three recombinant antigens markedly increases the sensitivity (72%) with only a minor reduction in specificity (82%). Thus, employing recombinant T. cruzi antigens to screen for T. cruzi infection has promise, but improvements in sensitivity must be made before widespread utilization is recommended. C1 NIAID,DAID,DIV EPIDEMIOL,BETHESDA,MD 20892. FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104. RP CETRON, MS (reprint author), UNIV WASHINGTON,DEPT MED,DIV INFECT DIS,SJ-10,SEATTLE,WA 98195, USA. NR 29 TC 16 Z9 16 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X J9 ACTA TROP JI Acta Trop. PD FEB PY 1992 VL 50 IS 3 BP 259 EP 266 DI 10.1016/0001-706X(92)90082-9 PG 8 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA HF100 UT WOS:A1992HF10000007 PM 1348602 ER PT J AU NASR, M CRADOCK, J JOHNSTON, MI AF NASR, M CRADOCK, J JOHNSTON, MI TI COMPUTER-ASSISTED STRUCTURE ACTIVITY CORRELATIONS OF HALODIDEOXYNUCLEOSIDE ANALOGS AS POTENTIAL ANTI-HIV DRUGS SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; ANTIVIRAL ACTIVITY; 2',3'-DIDEOXYNUCLEOSIDE ANALOGS; PYRIMIDINE DEOXYRIBONUCLEOSIDES; NUCLEOSIDE ANALOGS; 2',3'-DIDEOXYPURINE NUCLEOSIDES; HTLV-III/LAV; AGENTS; REPLICATION; INHIBITION AB Analysis of the structure-anti-HIV activity correlations of halo dideoxynucleosides (ddN's) in the public domain was accomplished through computer substructure searching, retrieval and sorting of in vitro anti-HIV data. In the survey, selectivity index (ratio of cytotoxicity to the potency in inhibiting HIV replication in vitro) was used to rank compounds in congeneric groups. Factors contributing to the anti-HIV activity, e.g. the nature and location of the halogen on the sugar or the base and its stereochemical configuration, could not be generalized for all the halogenated ddN's. Conclusions were drawn for specific classes within the pyrimidine and purine series, with compounds further divided into halo substitutions at the sugar 2',3',4'-positions or in the pyrimidine or purine ring systems. At the 3'-position, only a fluoro substitution enhanced the activity of the dideoxypyrimidine nucleosides. A 2'-ara fluoro substituent increased the activity of purine ddN's but decreased activity of pyrimidine ddN's. Halogenation of the side chain in acyclic adenine and 2,6-diaminopurine nucleosides improved their anti-HIV activity. Halo substitution at the 6- or 2'-ara position of selected purine ddN's resulted in compounds with increased lipophilicity, chemical stability and retention of anti-HIV activity. The number of halodideoxynucleosides tested as anti-HIV reagents suggested that this class of compounds is well studied. RP NASR, M (reprint author), NIAID,DIV AIDS,BETHESDA,MD 20892, USA. NR 56 TC 20 Z9 20 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1992 VL 8 IS 2 BP 135 EP 144 DI 10.1089/aid.1992.8.135 PG 10 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA HG489 UT WOS:A1992HG48900004 PM 1540402 ER PT J AU POLI, G FAUCI, AS AF POLI, G FAUCI, AS TI THE EFFECT OF CYTOKINES AND PHARMACOLOGICAL AGENTS ON CHRONIC HIV-INFECTION SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; TUMOR NECROSIS FACTOR; LONG TERMINAL REPEAT; DEFICIENCY SYNDROME AIDS; PROMONOCYTE CELL-LINE; FACTOR KAPPA-B; FACTOR-ALPHA; INTERFERON-ALPHA; PERIPHERAL-BLOOD; HTLV-III AB The ability of the human immunodeficiency virus (HIV) to replicate in CD4+ T lymphocytes and mononuclear phagocytes(MP) is strongly influenced by immunoregulatory cytokines. In the T cell system, interleukin-2 (IL-2) provides a mitogenic signal leading to both cell proliferation and virus replication. Among other HIV-inductive cytokines, only tumor necrosis factor-alpha or -beta (TNF-alpha/-beta) have been shown thus far to trigger virus expression both in T cells and MP. The mechanism of action of TNF involves the activation of the cellular transcription factor NF-kB which binds to specific consensus sequences present in the enhancer region of the HIV proviral LTR. In addition, several other cytokines (including colony stimulating factors, IL-1, IL-3, and IL-6) have demonstrated upregulatory effects on HIV production in MP, whereas nonimmune interferons (INF-alpha/-beta) have been shown to suppress HIV replication in T cells and MP by acting at different phases in the virus life cycle. Finally, cytokines such as TGF-beta, IFN-gamma, and IL-4 have demonstrated either upregulatory or suppressive effects on virus expression depending on the experimental conditions. This scenario indicates that HIV expression is under the control of a complex network of immunoregulatory cytokines, in addition to its own endogenous regulatory proteins, suggesting that new pharmacologic strategies may be aimed at either mimicking or interrupting cytokine-dependent virus expression. In this regard, a number of different physiologic and pharmacologic agents capable of interfering with cytokine-mediated events, including glucocorticoids, anti-oxidants, such as N-Acetyl-L-Cysteine (NAC), and retinoic acid (RA) have already been shown to profoundly affect HIV replication in vitro. RP POLI, G (reprint author), NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892, USA. NR 84 TC 239 Z9 243 U1 0 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1992 VL 8 IS 2 BP 191 EP 197 DI 10.1089/aid.1992.8.191 PG 7 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA HG489 UT WOS:A1992HG48900010 PM 1540407 ER PT J AU ADAMS, LD TOMASSELLI, AG ROBBINS, P MOSS, B HEINRIKSON, RL AF ADAMS, LD TOMASSELLI, AG ROBBINS, P MOSS, B HEINRIKSON, RL TI HIV-1 PROTEASE CLEAVES ACTIN DURING ACUTE INFECTION OF HUMAN LYMPHOCYTES-T SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; RETROVIRAL PROTEASES; PROTEINASE; COMPLEX; AIDS AB Actin, one of the most abundant proteins of the cell, is hydrolyzed by the human immunodeficiency virus type 1 (HIV-1) protease during acute infection of cultured human T lymphocytes. The actin fragments produced during the course of infection are identical to those obtained by recombinant HIV-1 protease digests of (1) a lysate from uninfected T lymphocytes and (2) globular actin itself. Hydrolysis by the HIV-1 protease of physiologically important host cellular proteins during infection may have important consequences relative to viral pathogenesis. C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. RP ADAMS, LD (reprint author), UPJOHN CO,BIOCHEM UNIT,KALAMAZOO,MI 49001, USA. NR 28 TC 38 Z9 38 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1992 VL 8 IS 2 BP 291 EP 295 DI 10.1089/aid.1992.8.291 PG 5 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA HG489 UT WOS:A1992HG48900023 PM 1540415 ER PT J AU WOERNER, AM KLUTCH, M LEVIN, JG MARCUSSEKURA, CJ AF WOERNER, AM KLUTCH, M LEVIN, JG MARCUSSEKURA, CJ TI LOCALIZATION OF DNA-BINDING ACTIVITY OF HIV-1 INTEGRASE TO THE C-TERMINAL HALF OF THE PROTEIN SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID MURINE LEUKEMIA-VIRUS; ESCHERICHIA-COLI; RETROVIRAL DNA; REVERSE-TRANSCRIPTASE; POL GENE; NUCLEOTIDE-SEQUENCE; AVIAN-SARCOMA; VIRAL-DNA; HTLV-III; INVITRO AB Human immunodeficiency virus type 1 (HIV-1) integrase (IN) is the viral protein required for integration of the HIV-1 genome into host cell DNA. A series of clones expressing portions of IN as lambda-cII fusion proteins has been constructed in an Escherichia coli expression system; a Southwestern procedure was used to examine binding of the expressed proteins to DNA oligonucleotides. Proteins expressed by clone pHIP106, encoding the entire IN protein but no other pol sequence, and pKNA101, which expresses an IN fusion protein containing 23 amino acids of HIV-1 reverse transcriptase at its amino terminus, exhibited similar levels of oligonucleotide binding. Little DNA sequence specificity was associated with binding activity and there was a preference for Mn2+ over Mg2+ and Ca2+. Interestingly, the protein expressed by an N-terminal clone containing nucleotides coding for IN amino acids 1-141 (including a conserved His-Cys box) was unable to bind oligonucleotide, whereas the protein expressed by a C-terminal clone containing nucleotides coding for amino acids 142-288 exhibited binding equivalent to that of full-length IN. The C-terminal protein was unreactive with a MAb to the lambda-cII leader peptide and with an antipeptide serum directed against amino acids 141-158. These results are consistent with the previously reported internal initiation of IN protein synthesis in E. coli at met 154, and indicate that the C-terminal clone does not express IN amino acids 142-153. These amino acids represent part of a conserved region termed D(35)E, containing amino acids 116-152, which has been implicated in IN DNA binding. Since the C-terminal sequences in IN bind DNA as well as the entire IN protein, our findings suggest that amino acids 154-288 are sufficient for DNA binding and that the conserved region, D(35)E, may not be required. C1 CTR BIOL EVALUAT & RES,DIV VIROL,BETHESDA,MD 20892. NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 47 TC 68 Z9 69 U1 0 U2 1 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1992 VL 8 IS 2 BP 297 EP 304 DI 10.1089/aid.1992.8.297 PG 8 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA HG489 UT WOS:A1992HG48900024 PM 1540416 ER PT J AU SIMOPOULOS, AP SALEM, N AF SIMOPOULOS, AP SALEM, N TI EGG-YOLK AS A SOURCE OF LONG-CHAIN POLYUNSATURATED FATTY-ACIDS IN INFANT-FEEDING SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE DOCOSAHEXAENOIC ACID; ARACHIDONIC ACID; OMEGA-3 FATTY ACIDS; INFANT FORMULA; BREAST MILK; PRETERM NEONATE; EGG YOLKS; INFANT NUTRITION ID RHESUS-MONKEYS; BRAIN; MILK; N-3; DEFICIENCY; RETINA AB In this paper we compare the fatty acid content of egg yolks from hens fed four different feeds as a source of docosahexaenoic acid to supplement infant formula. Greek eggs contain more docosahexacnoic acid (DHA, 22:6-omega-3) and less linoleic acid (LA, 18:2-omega-6) and alpha-linolenic acid (LNA, 18:3-omega-3) than do fish-meal or flax eggs. Two to three grams of Greek egg yolk may provide an adequate amount of DHA and arachidonic acid for a preterm neonate. Mean intake of breast milk at age 1 mo provides 250 mg long-chain omega-3 fatty acids. This amount can be obtained from < 1 yolk of a Greek egg (0.94), > 1 yolk of flax eggs (1.6) and fish-meal eggs (1.4), or 8.3 yolks of supermarket eggs. With proper manipulation of the hen's diets, eggs could be produced with fatty acid composition similar to that of Greek eggs. C1 NIAAA,MEMBRANE BIOCHEM & BIOPHYS LAB,BETHESDA,MD. RP SIMOPOULOS, AP (reprint author), CTR GENET NUTR & HLTH,2001 S ST NW,SUITE 530,WASHINGTON,DC 20009, USA. NR 27 TC 92 Z9 98 U1 0 U2 4 PU AMER SOC CLINICAL NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 1992 VL 55 IS 2 BP 411 EP 414 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA HB913 UT WOS:A1992HB91300013 PM 1734678 ER PT J AU FOSTER, WR BURTON, BT HUBBARD, VS AF FOSTER, WR BURTON, BT HUBBARD, VS TI GASTROINTESTINAL SURGERY FOR SEVERE OBESITY - PROCEEDINGS OF A NATIONAL-INSTITUTES-OF-HEALTH CONSENSUS DEVELOPMENT CONFERENCE - MARCH 25-27, 1991 BETHESDA, MD - INTRODUCTION SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Editorial Material C1 NIDDKD, NUTR SCI BRANCH, BETHESDA, MD 20892 USA. RP NIDDKD, OFF DIS PREVENT & TECHNOL TRANSFER, BETHESDA, MD 20892 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 1992 VL 55 IS 2 SU S BP 487 EP 487 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA HC918 UT WOS:A1992HC91800001 ER PT J AU RYAN, DH KOPECKY, KJ HEAD, D GREVER, MR SHIAER, SM LIPSCHITZ, DA HYNES, HE VIAL, RH VEITH, RW GUMBART, CH AF RYAN, DH KOPECKY, KJ HEAD, D GREVER, MR SHIAER, SM LIPSCHITZ, DA HYNES, HE VIAL, RH VEITH, RW GUMBART, CH TI ANALYSIS OF TREATMENT FAILURE IN ACUTE NONLYMPHOCYTIC LEUKEMIA PATIENTS OVER 50 YEARS OF AGE - A SOUTHWEST-ONCOLOGY-GROUP STUDY SO AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS LA English DT Article DE ACUTE NONLYMPHOCYTIC LEUKEMIA; GERIATRIC LEUKEMIA; REMISSION INDUCTION CHEMOTHERAPY ID ACUTE MYELOCYTIC-LEUKEMIA; ACUTE MYELOGENOUS LEUKEMIA; ADULT ACUTE-LEUKEMIA; INTENSIVE CHEMOTHERAPY; CYTOSINE-ARABINOSIDE; COMBINATION; VINCRISTINE AB Fourteen participating centers registered 33 patients on a Southwest Oncology Group Study of adults with acute nonlymphocytic leukemia (ANLL). Induction consisted of cytosine arabinoside 70 mg/m2 days 1-7 by continuous intravenous (i.v.) infusion, VP-16 50 mg/m2 i.v. over 1 hour days 1-3, and daunomycin 30 mg/m2 i.v. bolus days 1-3. Twenty five patients (median age 69 years) were evaluable for response. Eleven (44%) achieved a remission marrow but only 8 fulfilled both blood and marrow criteria for complete remission. Of the 11 patients with a remission marrow, there were no patients over 70 years of age. Major coexisting disease data were evaluated. Only 5 patients had no major coexisting disease and 4 of those 5 achieved a remission marrow. The study illustrates and underscores the following problems of remission induction in the elderly: (a) increased susceptibility to the stress of the induction period, with 6 patients (24%) dying before treatment day sixteen; (b) disease resistance to antileukemic therapy with persistent ANLL in 6 patients (24%), despite two induction courses; and (c) hematopoietic stem cell sensitivity in the elderly with marrow regeneration failure documented in 2 patients (8%) following induction. Acute nonlymphocytic leukemia in the elderly has a poor prognosis, and novel therapeutic approaches are warranted. C1 UNIV HOSP AUGUSTA, COMMUNITY CLIN ONCOL PROGRAM, AUGUSTA, GA USA. UNIV ARKANSAS, JOHN L MCCLELLAN CLIN MED SCI, FAYETTEVILLE, AR 72701 USA. COMMUNITY CLIN ONCOL PROGRAM, WICHITA, KS USA. LOUISIANA STATE UNIV, MED CTR, DEPT MED, NEW ORLEANS, LA 70112 USA. ST JUDE CHILDRENS RES HOSP, DEPT PATHOL & LAB MED, MEMPHIS, TN 38101 USA. NCI, DIV CANC TREATMENT, BETHESDA, MD 20892 USA. SW ONCOL GRP STAT CTR, SEATTLE, WA USA. RP RYAN, DH (reprint author), PENNINGTON BIOMED RES CTR, 6400 PERKINS RD, BATON ROUGE, LA 70808 USA. FU NCI NIH HHS [CA-13238, CA-36020, CA-37429] NR 19 TC 13 Z9 13 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0277-3732 J9 AM J CLIN ONCOL-CANC JI Am. J. Clin. Oncol.-Cancer Clin. Trials PD FEB PY 1992 VL 15 IS 1 BP 69 EP 75 DI 10.1097/00000421-199202000-00013 PG 7 WC Oncology SC Oncology GA GZ925 UT WOS:A1992GZ92500013 PM 1550082 ER PT J AU VANKRIEKEN, JHJM MEDEIROS, LJ PALS, ST RAFFELD, M KLUIN, PM AF VANKRIEKEN, JHJM MEDEIROS, LJ PALS, ST RAFFELD, M KLUIN, PM TI DIFFUSE AGGRESSIVE B-CELL LYMPHOMAS OF THE GASTROINTESTINAL-TRACT - AN IMMUNOPHENOTYPIC AND GENE REARRANGEMENT ANALYSIS OF 22 CASES SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE MALIGNANT LYMPHOMAS; GASTROINTESTINAL TRACT; IMMUNOPHENOTYPE; GENE REARRANGEMENTS ID NON-HODGKINS LYMPHOMA; C-MYC ONCOGENE; MOLECULAR-GENETICS; EXPRESSION; DIAGNOSIS; REGIONS; TISSUE; LFA-1; DNA AB Twenty-two diffuse aggressive B-cell lymphomas of the gastrointestinal tract were studied using light microscopic examination, immunohistochemical methods, and Southern blot analysis. The results suggest that diffuse aggressive B-cell gastrointestinal tract lymphomas may be divided into two groups: large cell lymphomas and small noncleaved cell lymphomas. Large cell lymphomas often involve the stomach; commonly express the lymphocyte adhesion molecules CD44, LFA-1 (CD11a and CD18), and CD54; and may express monotypic cytoplasmic immunoglobulin in approximately one third of cases. Southern blot analysis demonstrates rearrangements of the c-myc gene that do not co-migrate with rearrangements of the immunoglobulin heavy chain gene, as detected with a J(H) probe in approximately one half of the cases. Small noncleaved cell lymphomas typically involve the ileocecal region. In these lesions, monotypic cytoplasmic immunoglobulin is not detected, and the CD44 and LFA-1 molecules usually are not expressed, particularly in small noncleaved cell lymphomas of the Burkitt type. The CD54 antigen is positive in fewer than one half of cases. Southern blot studies often demonstrate rearrangements of the c-myc gene that co-migrate with immunoglobulin heavy chain gene rearrangements indicative of the t(8;14) chromosomal translocation, with the c-myc region translocated into the immunoglobulin heavy chain gene joining region. Thus, immunohistochemical and genotypic results, in accordance with the site of involvement and histologic findings, suggest a different pathogenesis for large cell lymphomas and small noncleaved cell lymphomas. The findings in large cell immunoblastic lymphomas are more akin to those of the large cell group. In addition, immunophenotypic and molecular data may be helpful in improving histologic classification when the morphologic findings are equivocal. C1 NCI,PATHOL LAB,HEMATOPATHOL SECT,BLDG 10,ROOM 2N108,BETHESDA,MD 20892. LEIDEN UNIV HOSP,DEPT PATHOL,2333 AA LEIDEN,NETHERLANDS. FREE UNIV AMSTERDAM,1007 MC AMSTERDAM,NETHERLANDS. RI van Krieken, Joannes/D-4138-2009 OI van Krieken, Joannes/0000-0001-6544-1040 NR 22 TC 16 Z9 16 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD FEB PY 1992 VL 97 IS 2 BP 170 EP 178 PG 9 WC Pathology SC Pathology GA HC499 UT WOS:A1992HC49900004 PM 1546685 ER PT J AU LINNOILA, RI JENSEN, SM STEINBERG, SM MULSHINE, JL EGGLESTON, JC GAZDAR, AF AF LINNOILA, RI JENSEN, SM STEINBERG, SM MULSHINE, JL EGGLESTON, JC GAZDAR, AF TI PERIPHERAL AIRWAY CELL MARKER EXPRESSION IN NON-SMALL-CELL LUNG-CARCINOMA - ASSOCIATION WITH DISTINCT CLINICOPATHOLOGICAL FEATURES SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE LUNG CANCER; ADENOCARCINOMA; PAPILLOLEPIDIC; CLARA CELL; TYPE-II CELL; SURFACTANT; SMOKING ID PULMONARY SURFACTANT APOPROTEINS; BRONCHIOLO-ALVEOLAR CARCINOMA; CLARA CELL; MONOCLONAL-ANTIBODIES; CANCER; LOCALIZATION; PROTEIN; DIFFERENTIATION; ADENOCARCINOMAS; LINES AB Paraffin sections of 247 primary and metastatic non-small cell lung carcinomas, the corresponding non-neoplastic lungs, and 75 other specimens were examined by immunohistochemical procedures using a panel of antibodies against the specific products of peripheral airway cells: the major surfactant-associated protein and 10-kD Clara cell protein. Non-small cell lung carcinoma tumors most frequently positive for either peripheral airway cell marker were adenocarcinomas (41%), especially those with papillolepidic growth pattern (56%), followed by large cell carcinomas (25%), other adenocarcinomas (22%), and squamous cell carcinomas (16%). Immunoreactivity was mainly focal and the expression of the two peripheral airway cell markers was discordant. The incidence of marker expression was similar in metastatic and primary non-small cell lung carcinoma. Other organs and their tumors were negative, with few exceptions. Non-mall cell lung carcinomas positive for peripheral airway cell markers were associated with younger age and less-intense smoking, and surfactant-associated protein reactivity was more common in women than in men. Peripheral airway cell markers were independent prognostic factors for survival and delayed development of metastases in patients with less-advanced disease. It is concluded that surfactant-associated protein and 10-kD Clara cell protein are specific markers for non-small cell lung carcinoma and peripheral airway cell differentiation and provide useful tools to study the pathogenesis, biology, and prognosis of non-small cell lung carcinoma. C1 NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. JOHNS HOPKINS UNIV HOSP,DEPT PATHOL,BALTIMORE,MD 21205. RP LINNOILA, RI (reprint author), USN HOSP,NCI,MED ONCOL BRANCH,NAVAL HOSP BLDG 8,ROOM 5101,BETHESDA,MD 20892, USA. NR 47 TC 93 Z9 93 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD FEB PY 1992 VL 97 IS 2 BP 233 EP 243 PG 11 WC Pathology SC Pathology GA HC499 UT WOS:A1992HC49900014 PM 1372146 ER PT J AU STORSAETER, J BLACKWELDER, WC HALLANDER, HO AF STORSAETER, J BLACKWELDER, WC HALLANDER, HO TI PERTUSSIS ANTIBODIES, PROTECTION, AND VACCINE EFFICACY AFTER HOUSEHOLD EXPOSURE SO AMERICAN JOURNAL OF DISEASES OF CHILDREN LA English DT Article ID BORDETELLA-PERTUSSIS; RESPONSES; INFECTION; CHILDREN; JAPAN; TRIAL; TOXIN AB During a randomized trial of acellular pertussis vaccines, significantly fewer recipients of a two-component vaccine (Japanese National Institute of Health [JNIH]-6) were diagnosed as primary or coprimary cases in households than either placebo recipients or those who received a monocomponent pertussis toxoid vaccine (JNIH-7). After household exposure to a culture-confirmed primary case, efficacy for JNIH-6 was estimated to be 35% (95% confidence interval, -14% to 5 7%) against any culture-confirmed disease and 58% (95% confidence interval, -6% to 84%) against clinical disease with 21 days or more of coughing spasms. The corresponding efficacy estimates for JNIH-7 were 67% (95% confidence interval, 32% to 80%) and 82% (95% confidence interval, 41 % to 96%). Differences between the JNIH-6 and JNIH-7 vaccines in efficacy after household exposure were not statistically significant. No association could be established between protection against pertussis after household exposure and serum levels of IgG antibody to pertussis toxin or filamentous hemagglutinin in vaccinated individuals, in either study children or other household members. C1 NIAID,BETHESDA,MD 20892. NATL BACTERIOL LAB,S-10521 STOCKHOLM,SWEDEN. RP STORSAETER, J (reprint author), KAROLINSKA INST,SACHS CHILDRENS HOSP,DEPT PEDIAT,BARNHALSOVARDEN,DRAKENBERGSGATAN 39,S-11741 STOCKHOLM,SWEDEN. NR 33 TC 54 Z9 54 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0002-922X J9 AM J DIS CHILD JI Am. J. Dis. Child. PD FEB PY 1992 VL 146 IS 2 BP 167 EP 172 PG 6 WC Pediatrics SC Pediatrics GA HC690 UT WOS:A1992HC69000010 PM 1733145 ER PT J AU HSING, AW MCLAUGHLIN, JK HRUBEC, Z BLOT, WJ AF HSING, AW MCLAUGHLIN, JK HRUBEC, Z BLOT, WJ TI TOBACCO USE AND PROSTATE-CANCER - 26-YEAR FOLLOW-UP OF UNITED-STATES VETERANS - REPLY SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Letter RP HSING, AW (reprint author), NCI,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD FEB 1 PY 1992 VL 135 IS 3 BP 328 EP 328 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA HJ918 UT WOS:A1992HJ91800018 ER PT J AU WALKER, C EVERITT, J BARRETT, JC AF WALKER, C EVERITT, J BARRETT, JC TI POSSIBLE CELLULAR AND MOLECULAR MECHANISMS FOR ASBESTOS CARCINOGENICITY SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article DE CELLULAR GROWTH FACTORS; OXYGEN RADICAL; MESOTHELIOMA; ONCOGENES; CANCER; MUTATION; GENOTOXICITY; CHROMOSOMES; CIGARETTE SMOKE; ASBESTOS ID HUMAN MESOTHELIAL CELLS; HUMAN-MALIGNANT MESOTHELIOMA; EPIDERMAL GROWTH-FACTOR; INDUCED LIPID-PEROXIDATION; TUMOR SUPPRESSOR GENES; HAMSTER EMBRYO CELLS; CHRYSOTILE ASBESTOS; CHROMOSOMAL-ABNORMALITIES; SHORT ARM; PLEURAL MESOTHELIOMA AB Asbestos fibers may exert their carcinogenic effects on mesothelial cells and bronchial epithelial cells by direct and indirect mechanisms. Direct effects can occur following the physical interaction of fibers with target cells or by the generation of free radicals from the fiber surface; indirect effects, following the interaction of fibers with inflammatory cells can result in the production of cellular mediators such as cytokines and various reactive oxygen species. As a result, target cells may be induced to proliferate and/or sustain genetic alterations, which lead to tumor development. C1 NIEHS,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709. RP WALKER, C (reprint author), CHEM IND INST TOXICOL,POB 12137,RES TRIANGLE PK,NC 27709, USA. NR 151 TC 88 Z9 89 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0271-3586 J9 AM J IND MED JI Am. J. Ind. Med. PD FEB PY 1992 VL 21 IS 2 BP 253 EP 273 DI 10.1002/ajim.4700210214 PG 21 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA HB479 UT WOS:A1992HB47900013 PM 1536158 ER PT J AU SIDRANSKY, E TSUJI, S MARTIN, BM STUBBLEFIELD, B GINNS, EI AF SIDRANSKY, E TSUJI, S MARTIN, BM STUBBLEFIELD, B GINNS, EI TI DNA MUTATION ANALYSIS OF GAUCHER PATIENTS SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE GAUCHER DISEASE; GLUCOCEREBROSIDASE; PHENOTYPE-GENOTYPE CORRELATIONS; MUTATION ANALYSIS ID ACID BETA-GLUCOSIDASE; GLUCOCEREBROSIDASE GENE; RESTRICTION SITE; DISEASE; AMPLIFICATION; HETEROGENEITY; FREQUENCY; DIAGNOSIS; DISORDER; TYPE-1 AB We evaluated 62 Gaucher patients to determine whether patients with similar phenotypes had the same DNA point mutations. Genomic DNA from these Gaucher patients was screened for the 3 most frequent single-point mutations, occurring in 69% of the 124 patient alleles, and resulting in changes in amino acids 370, 444, and 463. Many different genotypes were observed, at least one of which is present in all 3 types of Gaucher disease. No specific symptom complex could be correlated with a unique genotype. Even the more clinically homogeneous subgroups of Gaucher patients contained several genotypes. This study further emphasizes the need for caution in making clinical predictions on the basis of current genotype analysis, especially since one might not discern a fetus affected with type 2 disease by current DNA studies. The severity of involvement in type 1 disease could also not be predicted. Thus, even limiting our focus to 3 isolated common point mutations, a given genotype cannot be uniquely correlated with a specific prognosis. RP SIDRANSKY, E (reprint author), NIMH,CLIN NEUROSCI BRANCH,MOLEC GENET SECT,BLDG 10,ROOM 3D16,BETHESDA,MD 20892, USA. NR 25 TC 49 Z9 49 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD FEB 1 PY 1992 VL 42 IS 3 BP 331 EP 336 DI 10.1002/ajmg.1320420315 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA HA737 UT WOS:A1992HA73700014 PM 1536173 ER PT J AU FILLINGKATZ, MR LEVIN, SW PATRONAS, NJ KATZ, NNK AF FILLINGKATZ, MR LEVIN, SW PATRONAS, NJ KATZ, NNK TI TERMINAL TRANSVERSE LIMB DEFECTS ASSOCIATED WITH FAMILIAL CAVERNOUS ANGIOMATOSIS SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE FAMILIAL CAVERNOUS ANGIOMATOSIS; ANGIOMAS; DISRUPTION; LIMB DEFECT ID VASCULAR ABNORMALITIES; SCALP DEFECTS; MALFORMATIONS; DISRUPTION; HEMANGIOMA; ANOMALIES; POLAND; RETINA AB Terminal transverse limb defects rarely are reported as familial. Multiple pathogenetic mechanisms, including vascular disruption, have been proposed to account for these defects. We report on a family followed over the past 6 years known to have familial cavernous angiomatosis in which 2 relatives have similar terminal transverse defects at the mid-forearm. Multiple relatives have had episodic bleeding from intracranial cavernous angiomas, a distinct finding in this disorder. Other findings in this family include retinal cavernous angiomas (2 patients), a high incidence of skin angiomas (12 patients), cavernous angiomas of the soft tissue (2 patients), and a hepatic angioma (one patient). One of the 2 individuals with the limb defect was evaluated extensively. Magnetic resonance imaging of the forearm with the terminal transverse defect using gadolinium-DTPA enhancement showed abrupt termination of all structures distal to the normal radial and ulnar heads. We propose that familial cavernous angiomatosis may be a new cause of vascular disruption resulting in terminal transverse limb defects. C1 WALTER REED ARMY MED CTR,DEPT PEDIAT,EXCEPT FAMILY MEMBER PROGRAM,BLDG 38,WASHINGTON,DC 20307. UNIFORMED SERV UNIV HLTH SCI,DEPT PEDIAT,BETHESDA,MD 20814. UNIFORMED SERV UNIV HLTH SCI,DEPT SURG,DIV OPHTHALMOL,BETHESDA,MD 20814. NIH,WARREN G MAGNUSON CLIN CTR,DEPT RADIOL,BETHESDA,MD 20892. NIAAA,NEUROGENET LAB,BETHESDA,MD. NICHHD,HUMAN GENET BRANCH,BETHESDA,MD 20892. NR 31 TC 12 Z9 12 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD FEB 1 PY 1992 VL 42 IS 3 BP 346 EP 351 DI 10.1002/ajmg.1320420319 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA HA737 UT WOS:A1992HA73700018 PM 1536177 ER PT J AU NEWTON, RC LIMPUANGTHIP, P GREENBERG, S GAM, A NEVA, FA AF NEWTON, RC LIMPUANGTHIP, P GREENBERG, S GAM, A NEVA, FA TI STRONGYLOIDES-STERCORALIS HYPERINFECTION IN A CARRIER OF HTLV-I VIRUS WITH EVIDENCE OF SELECTIVE IMMUNOSUPPRESSION SO AMERICAN JOURNAL OF MEDICINE LA English DT Article ID IMMUNE-RESPONSES; IMMUNOLOGY; INFECTIONS; ANTIBODIES AB A patient with near fatal Strongyloides hyper-infection syndrome is briefly described. Investigation for possible risk factors for this parasitic infection disclosed that he was a carrier of human T-cell leukemia virus type I (HTLV-I), but without evidence of disease due to this retrovirus. Over the next few years, the patient's serum antibody levels of IgG to S. stercoralis larvae declined and became undetectable despite continued infection with the parasite. Repeated courses of appropriate treatment cleared the parasitic infection only temporarily. The patient was also found to have undetectable total serum IgE and a negative immediate hypersensitivity skin test to S. stercoralis antigens. Five of six other patients with HTLV-I-associated disease and with or without stronglyloidiasis were also found to have very low total serum IgE levels. It is postulated that HTLV-I infection in certain individuals may selectively impair immune responses that are critical in controlling strongyloidiasis. C1 NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892. HOWARD UNIV SERV,DIST COLUMBIA GEN HOSP,DEPT MED,WASHINGTON,DC. NCI,METAB BRANCH,BETHESDA,MD 20892. NR 19 TC 74 Z9 77 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD FEB PY 1992 VL 92 IS 2 BP 202 EP 208 DI 10.1016/0002-9343(92)90113-P PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA HE662 UT WOS:A1992HE66200015 PM 1543206 ER PT J AU PETERSON, CM JOVANOVICPETERSON, L MILLS, JL CONLEY, MR KNOPP, RH REED, GF AARONS, JH HOLMES, LB BROWN, Z VANALLEN, M SCHMELTZ, R METZGER, BE AF PETERSON, CM JOVANOVICPETERSON, L MILLS, JL CONLEY, MR KNOPP, RH REED, GF AARONS, JH HOLMES, LB BROWN, Z VANALLEN, M SCHMELTZ, R METZGER, BE TI THE DIABETES IN EARLY-PREGNANCY STUDY - CHANGES IN CHOLESTEROL, TRIGLYCERIDES, BODY-WEIGHT, AND BLOOD-PRESSURE SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE PREGNANCY; DIABETES; LIPIDS; BLOOD PRESSURE; WEIGHT ID WOMEN; MELLITUS; INSULIN; HEMOGLOBIN; INFANTS; MOTHERS AB This study examined changes in cholesterol, triglycerides, body weight, and blood pressure during pregnancy in 312 diabetic and 356 control women recruited within 21 days after conception. Cholesterol values rose in both groups but were significantly lower in diabetic women at each time point (166 vs 178 mg/dl at week 12, p = 0.0004). Triglyceride values also rose in both groups. Triglyceride levels did not differ between groups up to week 8 of gestation, but by weeks 10 to 12 they were significantly lower in diabetic women than in controls (75 vs 89 mg/dl at week 12, p = 0.0004). Although they were no heavier at entry, diabetic women gained significantly more weight between weeks 6 and 8 (p < 0.001), resulting in a mean difference between groups of 1 kg. Systolic blood pressure increased steadily and significantly in the diabetic but not the control women (115.8 +/- 16.2 SD vs 109.3 +/- 11.8 mm Hg, p = 0.0006 at term). Diastolic blood pressure was higher in diabetic women on entry (70.7 vs 67.3 mm Hg, p = 0.0006) and throughout gestation. Significant correlations were found in the diabetic group between maternal blood pressure and lipids and infant birth weight. These newly found differences in cholesterol and triglyceride levels, weight gain, and blood pressure between type l diabetic and control women during gestation may have long-term cardiovascular implications. C1 NICHHD, BETHESDA, MD 20892 USA. UNIV WASHINGTON, SCH MED, SEATTLE, WA 98195 USA. MAGEE WOMENS HOSP, PITTSBURGH, PA 15213 USA. BRIGHAM & WOMENS HOSP, BOSTON, MA 02115 USA. HOSP SICK CHILDREN, LONDON WC1N 3JH, ENGLAND. NORTHWESTERN UNIV, EVANSTON, IL 60201 USA. RP PETERSON, CM (reprint author), SANSUM MED RES FDN, 2219 BATH ST, SANTA BARBARA, CA 93105 USA. FU NCRR NIH HHS [RR-37]; NIDDK NIH HHS [DK-35816] NR 33 TC 32 Z9 32 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9378 EI 1097-6868 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD FEB PY 1992 VL 166 IS 2 BP 513 EP 518 PG 6 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA HE656 UT WOS:A1992HE65600008 PM 1536221 ER PT J AU NUGENT, R AF NUGENT, R TI ERYTHROMYCIN, MOTILIN, AND MECONIUM - REPLY SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Letter RP NUGENT, R (reprint author), NIH,PEDIAT ADOLESCENT & MATERNAL AIDS BRANCH,EXECUT PL S,ROOM 450W,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD FEB PY 1992 VL 166 IS 2 BP 767 EP 768 PG 2 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA HE656 UT WOS:A1992HE65600064 ER PT J AU COLE, TM MAYNARD, FM GRAITCER, PL APPLE, DF ACREE, KH BOEN, JR BONTKE, CF DITUNNO, JF FENDERSON, DA FINE, LJ FINE, PR FUHRER, MJ GLASS, DD GRAVES, W HELM, PA JAFFE, KM JETTE, A MACKENZIE, EJ MOSKOWITZ, J PERRY, J TRIESCHMANN, RB WALDREP, K WILLIAMS, TF BAER, K AF COLE, TM MAYNARD, FM GRAITCER, PL APPLE, DF ACREE, KH BOEN, JR BONTKE, CF DITUNNO, JF FENDERSON, DA FINE, LJ FINE, PR FUHRER, MJ GLASS, DD GRAVES, W HELM, PA JAFFE, KM JETTE, A MACKENZIE, EJ MOSKOWITZ, J PERRY, J TRIESCHMANN, RB WALDREP, K WILLIAMS, TF BAER, K TI EXECUTIVE SUMMARY - POSITION PAPER ON REHABILITATION OF PERSONS WITH INJURIES SO AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION LA English DT Article C1 NATL CTR ENVIRONM HLTH & INJURY CONTROL,ATLANTA,GA. SHEPHERD SPINAL CTR,ATLANTA,GA. CALIF DEPT HLTH SERV,SACRAMENTO,CA. UNIV MINNESOTA,SCH PUBL HLTH,MINNEAPOLIS,MN 55455. BAYLOR COLL MED,HOUSTON,TX 77030. THOMAS JEFFERSON UNIV,PHILADELPHIA,PA 19107. NIOSH,CINCINNATI,OH 45226. DEPT VET AFFAIRS MED CTR,MIAMI,FL. NIDRR,WASHINGTON,DC. UNIV TEXAS,SW MED CTR,DALLAS,TX 75230. UNIV WASHINGTON,SCH MED,SEATTLE,WA 98195. NEW ENGLAND RES INST,WATERTOWN,MA. JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. NIH,BETHESDA,MD 20892. RANCHO LOS AMIGOS MED CTR,DOWNEY,CA. KENT WALDREP NATL PARALYSIS FDN,DALLAS,TX. NIA,BETHESDA,MD 20892. MACRO INT INC,ATLANTA,GA. RP COLE, TM (reprint author), UNIV MICHIGAN,ANN ARBOR,MI 48109, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0894-9115 J9 AM J PHYS MED REHAB JI Am. J. Phys. Med. Rehabil. PD FEB PY 1992 VL 71 IS 1 BP 59 EP 61 PG 3 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA HE383 UT WOS:A1992HE38300016 ER PT J AU FLESSNER, MF DEDRICK, RL REYNOLDS, JC AF FLESSNER, MF DEDRICK, RL REYNOLDS, JC TI BIDIRECTIONAL PERITONEAL TRANSPORT OF IMMUNOGLOBULIN IN RATS - COMPARTMENTAL KINETICS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE MONOCLONAL ANTIBODY; INTERSTITIUM; LYMPHATIC; DIFFUSION; CONVECTION ID DISTRIBUTED MODEL; PLASMA TRANSPORT; PROTEIN; DIALYSIS; FLUID; LOCALIZATION; LYMPHATICS; CARCINOMA; BINDING AB Protein transport to and from fluid in the peritoneal cavity is observed during clinical procedures. Dialysate osmolality is a major determinant of net fluid flux into the cavity. We carried out experiments in rats to determine the plasma, peritoneal, and tissue concentrations of immunoglobulin (Ig) G resulting from either intravenous (iv) or intraperitoneal (ip) administration during hypertonic or isotonic dialyses. After iv injection of IgG, overall mass transfer into the cavity was not affected by the osmolality. After ip injection, tissue concentrations were dependent on the dialysis duration. Protein absorption from the hypertonic dialysate into the surrounding tissue was quantitatively less than the absorption from an isotonic dialysis solution at 20 min. By 200 min, total protein transport was not affected by dialysate osmolality. Lymphatic transport to the plasma amounted to 20-25% of the total protein loss from the peritoneal cavity; approximately 60% of the absorbed dose was found in tissues surrounding the cavity at both 20 and 200 min, with particularly high concentrations in parietal areas. We conclude that immunoglobulin transport in the peritoneal tissue, resulting from either iv or ip injection, is influenced by route of administration but is little affected by dialysate osmolality. Peritoneal absorption of proteins occurs directly into the surrounding tissue interstitial space as a result of hydrostatic pressure-driven convection and diffusion. C1 NIH,DEPT NUCL MED,BETHESDA,MD 20892. RP FLESSNER, MF (reprint author), NHLBI,NATL CTR RES RESOURCES,KIDNEY & ELECTROLYTE METAB LAB,BETHESDA,MD 20892, USA. NR 34 TC 35 Z9 36 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1992 VL 262 IS 2 BP F275 EP F287 PN 2 PG 13 WC Physiology SC Physiology GA HF163 UT WOS:A1992HF16300086 PM 1539687 ER PT J AU FANBURG, BL MASSARO, DJ CERUTTI, PA GAIL, DB BERBERICH, MA AF FANBURG, BL MASSARO, DJ CERUTTI, PA GAIL, DB BERBERICH, MA TI REGULATION OF GENE-EXPRESSION BY O2 TENSION SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Editorial Material ID OXYGEN; ERYTHROPOIETIN; INDUCTION C1 GEORGETOWN UNIV,MED CTR,WASHINGTON,DC 20007. SWISS INST EXPTL CANC RES,CH-1066 EPALINGES,SWITZERLAND. NHLBI,BETHESDA,MD 20892. RP FANBURG, BL (reprint author), NEW ENGLAND MED CTR HOSP,BOSTON,MA 02111, USA. NR 13 TC 33 Z9 33 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1992 VL 262 IS 2 BP L235 EP L241 PN 1 PG 7 WC Physiology SC Physiology GA HF161 UT WOS:A1992HF16100100 PM 1539680 ER PT J AU WADHWANI, KC MURPHY, VA SMITH, QR RAPOPORT, SI AF WADHWANI, KC MURPHY, VA SMITH, QR RAPOPORT, SI TI SATURABLE TRANSPORT OF MANGANESE(II) ACROSS BLOOD-NERVE BARRIER OF RAT PERIPHERAL-NERVE SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE BLOOD-BRAIN BARRIER; MUSCLE; SPINAL CORD; CARRIER-MEDIATED TRANSPORT; FACILITATED DIFFUSION; PERMEABILITY; SCIATIC NERVE ID BRAIN-BARRIER; FACILITATED TRANSPORT; SCIATIC-NERVE; PERMEABILITY; IRON; SYNTHETASE; CAPILLARY; GLUCOSE; SYSTEM; PLASMA AB To determine whether the blood-nerve barrier of the rat peripheral nerve transports manganese(II) (Mn) by a saturable mechanism similar to that found at the blood-brain barrier, we measured the uptake of Mn-54 from blood into desheathed sciatic nerve and into cerebral cortex of awake rats at different plasma concentrations of unlabeled Mn using an intravenous infusion technique. The unidirectional influx (J(in)) of Mn into sciatic nerve was facilitated and saturable, when steady-state plasma Mn ranged from 4 to 4,312 ng/ml (0.073-78.4-mu-M), as was the unidirectional influx of Mn into the cerebral cortex. Michaelis-Menten constants (K(m) and V(max)) and the passive diffusion constant (K(d)), determined by nonlinear least squares, were as follows: for the blood-nerve barrier (sciatic nerve) K(m) = 4.7-mu-M, V(max) = 0.56 x 10(-3) nmol.s-1.g wet wt-1, and K(d) = 6.3 x 10(-6) ml.s-1.g wet wt-1; for the blood-brain barrier (cerebral cortex) K(m) = 1.0-mu-M, V(max) = 0.40 x 10(-3) nmol.s-1.g wet wt-1, and K(d) = 0.3 x 10(-6) ml.s-1.g wet wt-1. The results demonstrate facilitated concentration-dependent mechanisms of transport of Mn at the blood-nerve and blood-brain barriers. RP WADHWANI, KC (reprint author), NIA,NEUROSCI LAB,BLDG 10,RM 6C103,BETHESDA,MD 20892, USA. NR 41 TC 18 Z9 18 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1992 VL 262 IS 2 BP R284 EP R288 PN 2 PG 5 WC Physiology SC Physiology GA HF163 UT WOS:A1992HF16300064 PM 1539737 ER PT J AU LEIBENLUFT, E WEHR, TA AF LEIBENLUFT, E WEHR, TA TI IS SLEEP-DEPRIVATION USEFUL IN THE TREATMENT OF DEPRESSION SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID ANTIDEPRESSANT RESPONSE; ENDOGENOUS-DEPRESSION; AFFECTIVE-ILLNESS; MAJOR DEPRESSION; THERAPY; CLOMIPRAMINE; PREDICTION; PROLACTIN; SECRETION; SYMPTOMS AB Objective: The authors critically reviewed the literature on clinical applications of sleep deprivation in the treatment of depression. Data Collection: They included all studies using sleep deprivation for clinical purposes, with the exception of treatment studies that did not provide follow-up beyond a night of recovery sleep. They focused on six uses of sleep deprivation: 1) to potentiate response to antidepressant medication (13 studies), 2) to hasten the onset of action of antidepressant medication or lithium (five studies), 3) to prevent recurrent mood cycles (four studies), 4) as an alternative to antidepressant medication (five studies), 5) as a diagnostic probe (two studies), and 6) to predict response to antidepressant medication nine studies). Findings: Although the literature appears to demonstrate the efficacy of sleep deprivations as a potentiation strategy, these treatment studies have substantial methodological shortcomings. Well-designed pilot studies indicate that sleep deprivation may hasten the onset of action of thymoleptic medications. Sleep deprivation may prevent premenstrual mood swings, and response to sleep deprivation may differentiate depressive pseudodementia from primary degenerate dementia with depression. Studies attempting to use sleep deprivation to predict response to antidepressant medication have yielded inconsistent results. Conclusions: Given the noninvasive nature of sleep deprivation, it would be useful to determine if even a small subset of refractory patients respond to it. The authors suggest future research directions to determine the usefulness of this potential treatment. RP LEIBENLUFT, E (reprint author), NIMH,CLIN PSYCHOBIOL BRANCH,BLDG 10,RM 4S-239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 71 TC 103 Z9 105 U1 2 U2 6 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD FEB PY 1992 VL 149 IS 2 BP 159 EP 168 PG 10 WC Psychiatry SC Psychiatry GA HC079 UT WOS:A1992HC07900002 PM 1734735 ER PT J AU RIEVES, RD GOFF, J WU, T LARIVEE, P LOGUN, C SHELHAMER, JH AF RIEVES, RD GOFF, J WU, T LARIVEE, P LOGUN, C SHELHAMER, JH TI AIRWAY EPITHELIAL-CELL MUCIN RELEASE - IMMUNOLOGICAL QUANTITATION AND RESPONSE TO PLATELET-ACTIVATING-FACTOR SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID FACTOR STIMULATES SECRETION; ARACHIDONIC-ACID; MONOCLONAL-ANTIBODIES; RESPIRATORY GLYCOCONJUGATE; BROMOPHENACYL BROMIDE; CULTURE; MECHANISM; ANTIGENS; BRONCHOCONSTRICTION; EXPLANTS AB Mucus production is an integral component of airway mucosal inflammation. Platelet-activating factor (PAF) is a phospholipid mediator implicated in the pathogenesis of many inflammatory processes, including airway inflammation. PAF functions as a mucus secretagogue when mucus is quantitated as radiolabeled glycoconjugates released from airway organ cultures. To more directly assess the interaction of PAF and airway epithelial mucous cell secretion, we used primary feline tracheal epithelial cell cultures and an immunoassay for a specific mucous cell secretory vesicle component. Cultured tracheal epithelial cells were shown to synthesize and secrete glycoconjuates with mucin characteristics. These mucin-type glycoconjugates were immunoreactive with a mucous cell-specific antibody. Localization of this antibody to components of the secretory vesicles of cultured epithelial cells was confirmed by electron microscopic immunogold labeling. Using this monoclonal antibody, an immunoassay was developed to quantitate release of immunoreactive material into cell culture media. Exposure of cultures to PAF produced a concentration-dependent, prompt release of immunoreactive material. Concentration-dependent inhibition of this effect was demonstrated by coincubation with the PAF receptor antagonists, WEB 2086 and Ro 19-3704. A component of the signal transduction pathway for PAF effects was studied in cultured tracheal epithelial cells by coincubation of PAF with nordihydroguaiaretic acid (NDGA), a combined lipoxygenase and cyclooxygenase inhibitor, or p-bromophenacyl bromide (BPB), an inhibitor of cellular arachidonic acid release. Both NDGA and BPB blocked PAF-stimulated mucin release in a concentration-dependent manner. These studies demonstrate a direct airway epithelial mucous cell secretagogue effect that appears to be dependent upon airway epithelial PAF receptors and altered cellular lipid metabolism. These findings suggest a direct and potent mechanism for goblet cell secretion during airway inflammation. C1 NIH,DEPT CRIT CARE MED,BLDG 10,10 ROOM 7D43,BETHESDA,MD 20892. NR 47 TC 33 Z9 34 U1 0 U2 1 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD FEB PY 1992 VL 6 IS 2 BP 158 EP 167 PG 10 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA HC989 UT WOS:A1992HC98900007 PM 1540379 ER PT J AU WU, T MULLOL, J RIEVES, RD LOGUN, C HAUSFIELD, J KALINER, MA SHELHAMER, JH AF WU, T MULLOL, J RIEVES, RD LOGUN, C HAUSFIELD, J KALINER, MA SHELHAMER, JH TI ENDOTHELIN-1 STIMULATES EICOSANOID PRODUCTION IN CULTURED HUMAN NASAL-MUCOSA SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID AIRWAY EPITHELIAL-CELLS; SMOOTH-MUSCLE CELLS; GUINEA-PIG AIRWAYS; ARACHIDONIC-ACID; VASOCONSTRICTOR PEPTIDE; RELEASE; BRONCHOCONSTRICTOR; CYCLOOXYGENASE; METABOLISM; VASODILATION AB Endothelin (ET) has been shown to contract both vascular and nonvascular smooth muscle and to stimulate human nasal glandular secretion of serous and mucous cell products. Some effects of ET are thought to be mediated by eicosanoid production. To explore the direct effect of ET on arachidonate metabolism in cultured human nasal mucosal explants, eicosanoids were measured after ET-1 stimulation. After labeling the explants with [H-3]arachidonic acid (AA), supernatant from control and ET-1-treated explants were fractionated by reverse-phase high-performance liquid chromatography (HPLC). The resulting elution pattern suggested the release of prostaglandin (PG) E2 and AA in response to ET-1 stimulation. Radioimmunoassay after HPLC resolution confirmed that ET-1 induced a significantly increased release of PGE2 as well as PGD2, PGF2-alpha, thromboxane B2, and 15-hydroxyeicosatetraenoic acid (15-HETE). Although significant amounts of 15-HETE were generated, cyclooxygenase product generation was most remarkable. Eicosanoid release after ET-1 exposure (10 to 0.1-mu-M) is concentration dependent and occurs within 1 h. Whereas 15-HETE release was maximal at 4 h, prostanoid production was maximal 1 h after exposure to ET-1. Other assayed AA metabolites, including the peptidoleukotrienes, did not significantly change after ET-1 stimulation. We conclude that ET-1 induces the release of predominantly cyclooxygenase products from cultured human nasal mucosal explants. C1 NIH, DEPT CRIT CARE MED, BLDG 10, ROOM 7D43, BETHESDA, MD 20892 USA. NIH, CLIN INVEST LAB, ALLERG DIS SECT, BETHESDA, MD 20892 USA. WASHINGTON HOSP CTR, DEPT FACIAL PLAST & RECONSTRUCT SURG, WASHINGTON, DC 20010 USA. NR 48 TC 38 Z9 39 U1 0 U2 0 PU AMER THORACIC SOC PI NEW YORK PA 61 BROADWAY, FL 4, NEW YORK, NY 10006 USA SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD FEB PY 1992 VL 6 IS 2 BP 168 EP 174 PG 7 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA HC989 UT WOS:A1992HC98900008 PM 1311593 ER PT J AU PREMKUMAR, A SANDERS, L MARINCOLA, F FEUERSTEIN, I CONCEPCION, R SCHWARTZENTRUBER, D AF PREMKUMAR, A SANDERS, L MARINCOLA, F FEUERSTEIN, I CONCEPCION, R SCHWARTZENTRUBER, D TI VISCERAL METASTASES FROM MELANOMA - FINDINGS ON MR IMAGING SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article; Proceedings Paper CT 1991 ANNUAL MEETING OF THE AMERICAN ROENTGEN RAY SOC CY MAY, 1991 CL BOSTON, MA SP AMER ROENTGEN RAY SOC ID MALIGNANT-MELANOMA; RESONANCE; CT; PATTERN; TUMORS AB Typical ocular and CNS melanomas are hyperintense on T1-weighted MR images and hypointense on T2-weighted MR images. We performed MR imaging in 48 patients with melanoma metastatic to visceral organs. Images were reviewed retrospectively in order to determine whether there were predominant MR features specific for visceral melanoma and to see if visceral metastases have MR characteristics similar to metastases in the CNS. Eleven patients also were examined after injection of gadopentetate dimeglumine to evaluate the enhancement characteristics of these tumors. Two hundred sixty-one lesions were found. Lesions were classified according to their signal intensities relative to uninvolved liver on T1-weighted, T2-weighted, and short TI inversion recovery (STIR) pulse sequences. Most commonly, lesions were either hypointense or isointense on T1-weighted sequences and hyperintense on T2-weighted and STIR sequences (185 lesions). Less frequently, lesions were hyperintense on T1-weighted sequences and hypointense or isointense on T2-weighted and STIR sequences (59 lesions). A mixed pattern was seen on T1- and T2-weighted sequences in 17 lesions. The patterns did not correlate with lesion size. Of the three sequences studied by subjective comparison, the STIR sequence in our series had the highest sensitivity for lesion detection and yielded the highest lesion conspicuity. Injection of gadopentetate dimeglumine in 11 patients did not increase either the number or the conspicuity of lesions seen. Our results show that visceral metastases from melanoma have a wide variety of appearances on MR images. The STIR sequence appears to be optimal, and the metastases do not enhance with gadopentetate dimeglumine. C1 COLUMBIA PRESBYTERIAN MED CTR,DEPT DIAGNOST RADIOL,NEW YORK,NY 10032. NCI,SURG BRANCH,BETHESDA,MD 20892. RP PREMKUMAR, A (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,BLDG 10,ROOM 1C660,BETHESDA,MD 20892, USA. NR 20 TC 32 Z9 32 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR, SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD FEB PY 1992 VL 158 IS 2 BP 293 EP 298 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA HB058 UT WOS:A1992HB05800011 PM 1729784 ER PT J AU SUNDEEN, JT LONGO, DL JAFFE, ES AF SUNDEEN, JT LONGO, DL JAFFE, ES TI CD5 EXPRESSION IN B-CELL SMALL LYMPHOCYTIC MALIGNANCIES - CORRELATIONS WITH CLINICAL PRESENTATION AND SITES OF DISEASE SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE SMALL LYMPHOCYTIC LYMPHOMAS; MUCOSA-ASSOCIATED LYMPHOID TISSUE; CD5; B-CELLS; MALIGNANT LYMPHOMA, EXTRANODAL ID INTERMEDIATE DIFFERENTIATION; GASTRIC LYMPHOMA; NEOPLASMS; TISSUE; LEU-1 AB The authors examined the relationship between CD5 antigen expression and a nodal or extranodal presentation for three subtypes of low-grade non-Hodgkin's lymphoma: small lymphocytic (23 cases), small lymphocytic with plasmacytoid differentiation (10 cases), and lymphocytic lymphoma of intermediate differentiation (IDL) (29 cases). Antigen expression was studied by the avidinbiotin complex immunoperoxidase technique in frozen sections and correlated with expression of other B- and T-cell markers. Lack of CD5 expression was significantly associated with extranodal presentation among the overall study group (p < 0.001), as well as for those with small lymphocytic lymphoma and IDL, but not for those presenting with small lymphocytic lymphomas with plasmacytoid differentiation (p < 0.21). Eleven patients presented exclusively with extranodal disease involving lung and respiratory tract, skin and subcutaneous tissue, salivary gland, stomach, conjunctive, and uterus. All such lesions were CD5 negative and had been classified as small lymphocytic (four cases), small lymphocytic-plasmacytoid (four cases), and IDL (three cases). Retrospective review of these 11 cases demonstrated common histologic features described as characteristic of lymphomas of mucosa-associated lymphoid tissue (MALT). Two additional patients presented with disseminated nodal disease and involvement of gastrointestinal tract and oropharynx; both were CD5 positive. These findings support the concept that at least two antigenically distinct B-cell subpopulations may be involved in pathogenesis of low-grade small lymphocytic malignancics. C1 NCI,PATHOL LAB,HEMATOPATHOL SECT,BLDG 10,ROOM 2N202,BETHESDA,MD 20892. NCI,BIOL RESPONSE MODIFIERS PROGRAM,BETHESDA,MD 20892. NR 22 TC 54 Z9 54 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD FEB PY 1992 VL 16 IS 2 BP 130 EP 137 DI 10.1097/00000478-199202000-00005 PG 8 WC Pathology; Surgery SC Pathology; Surgery GA HB584 UT WOS:A1992HB58400005 PM 1370753 ER PT J AU CLARK, CG DIAMOND, LS AF CLARK, CG DIAMOND, LS TI COLONIZATION OF THE UTERUS BY THE ORAL PROTOZOAN ENTAMOEBA-GINGIVALIS SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article AB Pap smears occasionally reveal protozoa of the genus Entamoeba in the uterus of intrauterine device (IUD) users, but definitive identification of the species involved has not been possible. Using riboprinting, a technique that compares ribosomal RNA gene sequences, we present evidence that the organism is Entamoeba gingivalis, an inhabitant of the mouth. Colonization most likely occurs via orogenital contact and requires the presence of an IUD and a concomitant bacterial infection. RP CLARK, CG (reprint author), NIAID,PARASIT DIS LAB,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. RI Clark, C Graham/H-3683-2011 OI Clark, C Graham/0000-0002-0521-0977 NR 14 TC 8 Z9 9 U1 0 U2 1 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DRIVE SUITE 130, MCLEAN, VA 22101 SN 0002-9637 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD FEB PY 1992 VL 46 IS 2 BP 158 EP 160 PG 3 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA HH014 UT WOS:A1992HH01400009 PM 1539749 ER PT J AU ELSON, HF GENTRY, MK DOCTOR, BP AF ELSON, HF GENTRY, MK DOCTOR, BP TI A MICROTITER ASSAY FOR DETERMINING PROTEIN, ACETYLCHOLINESTERASE ACTIVITY, AND G418 (NEOMYCIN) RESISTANCE IN CULTURED-CELLS SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID DYE-BINDING; QUANTITATION; FRACTIONS; SDS C1 NHLBI, MOLEC BIOL LAB, BETHESDA, MD 20892 USA. RP WALTER REED ARMY MED CTR, DEPT BIOCHEM, WASHINGTON, DC 20307 USA. NR 17 TC 4 Z9 4 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 EI 1096-0309 J9 ANAL BIOCHEM JI Anal. Biochem. PD FEB 1 PY 1992 VL 200 IS 2 BP 268 EP 272 DI 10.1016/0003-2697(92)90464-I PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA HB926 UT WOS:A1992HB92600010 PM 1378703 ER PT J AU Bayne, K Dexter, S Mainzer, H McCully, C Campbell, G Yamada, F AF Bayne, K. Dexter, S. Mainzer, H. McCully, C. Campbell, G. Yamada, F. TI THE USE OF ARTIFICIAL TURF AS A FORAGING SUBSTRATE FOR INDIVIDUALLY HOUSED RHESUS MONKEYS (MACACA MULATTA) SO ANIMAL WELFARE LA English DT Article DE animal welfare; environmental enrichment; psychological well-being AB The provisioning of foraging opportunities to primates has been shown to be an effective means of enriching the laboratory environment. In this study artificial turf was used as the substrate for a particulate food given to the subjects as an environmental enrichment technique. Eight rhesus monkeys exhibited a significant reduction in behavioural pathology when allowed to extend the amount of time they spent in consummatory activities. An increasing trend in time spent foraging with a concomitant decline in aberrant behaviour over a period of six months was particularly noteworthy. No significant difference in preference for particulate monkey chow or more flavourful particulate food treats was expressed by the primates. C1 [Bayne, K.; Dexter, S.; Mainzer, H.] NIH, Off Anim Care & Use, Off Director, Bethesda, MD 20892 USA. [McCully, C.] NCI, Pediat Branch, NIH, Bethesda, MD 20892 USA. [Campbell, G.; Yamada, F.] NIH, Div Comp Res & Technol, Bethesda, MD 20892 USA. RP Bayne, K (reprint author), NIH, Off Anim Care & Use, Off Director, 9000 Rockville Pike,Bldg 14D,Room 313, Bethesda, MD 20892 USA. NR 26 TC 41 Z9 41 U1 1 U2 10 PU UNIV FEDERATION ANIMAL WELFARE PI WHEATHAMPSTEAD PA OLD SCHOOL, BREWHOUSE HILL, WHEATHAMPSTEAD AL4 8AN, HERTS, ENGLAND SN 0962-7286 J9 ANIM WELFARE JI Anim. Welf. PD FEB PY 1992 VL 1 IS 1 BP 39 EP 53 PG 15 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA V22QE UT WOS:000208288900005 ER PT J AU FLACK, MR OLDFIELD, EH CUTLER, GB ZWEIG, MH MALLEY, JD CHROUSOS, GP LORIAUX, DL NIEMAN, LK AF FLACK, MR OLDFIELD, EH CUTLER, GB ZWEIG, MH MALLEY, JD CHROUSOS, GP LORIAUX, DL NIEMAN, LK TI URINE FREE CORTISOL IN THE HIGH-DOSE DEXAMETHASONE SUPPRESSION TEST FOR THE DIFFERENTIAL-DIAGNOSIS OF THE CUSHING SYNDROME SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE DIAGNOSIS, DIFFERENTIAL; CUSHING SYNDROME; PITUITARY DISEASE; DEXAMETHASONE; HYDROCORTISONE ID LABORATORY TESTS; UPDATE AB Objective: To develop criteria for interpreting the high-dose dexamethasone suppression test using urine free cortisol as an end point for the differential diagnosis of the Cushing syndrome. Design: Retrospective review. Setting: Inpatient research ward. Patients: Patients (118) with surgically confirmed causes of the Cushing syndrome: 94 with pituitary disease, 14 with primary adrenal disease, and 10 with ectopic adrenocorticotropic hormone (ACTH) secretion. Main Outcome Measures: The sensitivity, specificity, and diagnostic accuracy were determined for the high-dose dexamethasone suppression test using urine free cortisol and using 17-hydroxysteroid excretion. For each analysis, patients with pituitary disease were considered to be "diseased" and patients with nonpituitary disease were considered to be "non-diseased". The level of suppression that gave 100% specificity was determined for each steroid. Results: The accuracy of urine free cortisol when used as an end point in the high-dose dexamethasone suppression test was equivalent to that of 17-hydroxysteroid excretion. At all levels of sensitivity and specificity, however, the degree of suppression of urine free cortisol used for the diagnosis of pituitary disease was greater than that of 17-hydroxysteroid excretion. The likelihood ratios for pituitary disease based on urine free cortisol suppression of > 50%, of > 80%, and of > 90% were 4.2, 10.1, and "infinite," respectively. Suppression of urine free cortisol greater than 90% or suppression of 17-hydroxysteroid excretion greater than 64% was associated with 100% specificity. When these criteria were combined, the percentage of correct predictions (102 of 118 [86%; 95% Cl, 78% to 92%]) was higher than that obtained using either steroid alone (89 of 118 [75%; Cl, 65% to 83%]) (P = 0.009) and higher than that obtained using the traditional criterion of 50% suppression for 17-hydroxysteroid excretion (95 of 118 [80%; Cl, 71% to 87%]) (P = 0.016). Conclusions: In the high-dose dexamethasone suppression test, the degree of suppression of urine free cortisol used for the diagnosis of pituitary disease is greater than that traditionally used for 17-hydroxysteroid excretion. The diagnostic performance of the test is improved by measuring both urine free cortisol and 17-hydroxysteroid excretion and by requiring greater suppression of both steroids. RP FLACK, MR (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLD 10,ROOM 10N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 31 TC 113 Z9 120 U1 0 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD FEB 1 PY 1992 VL 116 IS 3 BP 211 EP 217 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA HB356 UT WOS:A1992HB35600006 PM 1728204 ER PT J AU LUDLOW, CL VANPELT, F KODA, J AF LUDLOW, CL VANPELT, F KODA, J TI CHARACTERISTICS OF LATE RESPONSES TO SUPERIOR LARYNGEAL NERVE-STIMULATION IN HUMANS SO ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY LA English DT Article DE CRICOTHYROID MUSCLE; REFLEX; SUPERIOR LARYNGEAL NERVE ID REFLEX; INHIBITION; COUGH AB To characterize human thyroarytenoid and cricothyroid muscle responses to stimulation of the internal (sensory) and external (motor) branches of the superior laryngeal nerve (SLN), three awake subjects were studied at rest and during muscle activation with stimulation at different levels. When only the external branch was stimulated, direct cricothyroid muscle responses were obtained without responses in either thyroarytenoid muscle. When only the internal branch was stimulated, no cricothyroid responses were obtained, but two late thyroarytenoid responses occurred (R1 and R2). The R1 response was usually ipsilateral and had a mean onset latency of 18 milliseconds, while the R2 response was bilateral and occurred between 66 and 70 milliseconds. Both responses tended to decrease in latency and increase in amplitude with increased stimulation level. The similarity of R1 to the adductor response and R2 to other late responses is discussed. RP LUDLOW, CL (reprint author), NIDCD,DIV INTRAMURAL RES,VOICE & SPEECH SECT,BLDG 10,ROOM 5038,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Ludlow, Christy/0000-0002-2015-6171 NR 20 TC 63 Z9 64 U1 0 U2 0 PU ANNALS PUBL CO PI ST LOUIS PA 4507 LACLEDE AVE, ST LOUIS, MO 63108 SN 0003-4894 J9 ANN OTO RHINOL LARYN JI Ann. Otol. Rhinol. Laryngol. PD FEB PY 1992 VL 101 IS 2 BP 127 EP 134 PN 1 PG 8 WC Otorhinolaryngology SC Otorhinolaryngology GA HD332 UT WOS:A1992HD33200004 PM 1739256 ER PT J AU DOHERTY, GM DOPPMAN, JL MILLER, DL GEE, MS MARX, SJ SPIEGEL, AM AURBACH, GD PASS, HI BRENNAN, MF NORTON, JA AF DOHERTY, GM DOPPMAN, JL MILLER, DL GEE, MS MARX, SJ SPIEGEL, AM AURBACH, GD PASS, HI BRENNAN, MF NORTON, JA TI RESULTS OF A MULTIDISCIPLINARY STRATEGY FOR MANAGEMENT OF MEDIASTINAL PARATHYROID ADENOMA AS A CAUSE OF PERSISTENT PRIMARY HYPERPARATHYROIDISM SO ANNALS OF SURGERY LA English DT Article ID RECURRENT HYPERPARATHYROIDISM; ANGIOGRAPHIC ABLATION; UNDERGONE SURGERY; LOCALIZATION AB Persistent primary hyperparathyroidism due to mediastinal parathyroid adenoma was effectively treated by either angiographic ablation or median sternotomy in this study of 49 patients managed at the National Institutes of Health since 1977. Each patient presented here with symptomatic persistent primary hyperparathyroidism after failed initial surgical procedures done at other institutions. Each patient underwent extensive parathyroid localization procedures, including selective angiography, and most had a parathyroid adenoma localized to the mediastinum. Angiographic ablation, the deliberate injection of large doses of contrast material into the artery that selectively perfuses the adenoma, was initially successful in 22 of 30 procedures (37%) in 27 patients. Long-term control of persistent primary hyperparathyroidism was achieved in 17 of 27 patients (63%) by angiographic ablation. Each unsuccessful ablation could be easily salvaged by surgical resection. Surgical resection of the parathyroid adenoma by median sternotomy achieved immediate success in 24 of 24 procedures (p2 < 0.02 versus ablation), and long-term cure in 23 of 23 evaluable patients (p2 < 0.001 versus ablation). However, ablation did have benefits for the patients in whom it was successfully performed. It was associated with a significantly shorter hospital stay (median, 6 days versus 9 days for sternotomy, p2 < 0.003), much less pain, and easier recuperation. Complications of each procedure were transient and similar in both groups. Operative resection is the most effective single means to eradicate mediastinal parathyroid adenoma; however, angiographic ablation can provide similar long-term control of hyperparathyroidism in 63% of patients with less pain and shorter convalescence than that seen in patients after median sternotomy. Our results suggest that angiographic ablation should be attempted as the initial procedure for patients with persistent primary hyperparathyroidism caused by an angiographically identified mediastinal parathyroid adenoma. Operation can be reserved for those who fail ablation. C1 NCI,SURG BRANCH,SURG METABOL SECT,BLDG 10,ROOM 2B07,BETHESDA,MD 20892. NIDDKD,MOLEC PATHOPHYSIOL BRANCH,BETHESDA,MD. NIDDKD,DEPT DIAGNOST RADIOL,BETHESDA,MD. NIDDKD,WARREN GRANT MAGNUSON CLIN CTR,METAB DIS BRANCH,BETHESDA,MD. NCI,SURG BRANCH,THORAC ONCOL SECT,BETHESDA,MD 20892. MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021. NR 16 TC 50 Z9 51 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-4932 J9 ANN SURG JI Ann. Surg. PD FEB PY 1992 VL 215 IS 2 BP 101 EP 106 DI 10.1097/00000658-199202000-00002 PG 6 WC Surgery SC Surgery GA HD462 UT WOS:A1992HD46200002 PM 1546895 ER PT J AU WELSH, LE GAYDOS, CA QUINN, TC AF WELSH, LE GAYDOS, CA QUINN, TC TI INVITRO EVALUATION OF ACTIVITIES OF AZITHROMYCIN, ERYTHROMYCIN, AND TETRACYCLINE AGAINST CHLAMYDIA-TRACHOMATIS AND CHLAMYDIA-PNEUMONIAE SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID SUSCEPTIBILITIES; INFECTIONS; STRAIN; TWAR AB The in vitro activities of azithromycin (CP-62,993; Pfizer), erythromycin, and tetracycline were evaluated by inhibiting Chlamydia trachomatis and Chlamydia pneumoniae, formerly TWAR, propagation in vitro in McCoy cells, HeLa cells, and HL cells. Eleven clinical isolates of C. trachomatis (serovars D, E, F, J, K, and L2) and four strains of C. pneumoniae were tested with an inoculum of 10(3) inclusion-forming units in a 96-well microtiter plate. The MIC ranges of these antimicrobial agents against C. trachomatis were as follows: azithromycin, 0.125 to 0.5-mu-g/ml; erythromycin, 0.25 to 0.1-mu-g/ml; and tetracycline, 0.0625 to 1.0-mu-g/ml. The MBC ranges, calculated from passage into antibiotic-free medium, were as follows: azithromycin, 0.125 to 4.0 4.0-mu-g/ml; erythromycin, 0.5 to 8.0-mu-g/ml; and tetracycline, 0.0625 to 4.0-mu-g/ml. The MIC ranges for C. pneumoniae in both HeLa and HL cells were as follows: azithromycin, 0.125 to 1.0-mu-g/ml; erythromycin, 0.0625 to 1.0-mu-g/ml; and tetracycline, 0.125 to 1.0-mu-g/ml. The MBC ranges were as follows: azithromycin, 0.25 to 1.0-mu-g/ml; erythromycin, 0.25 to 1.0-mu-g/ml; and tetracycline, 0.125 to 4.0-mu-g/ml. From the results of this in vitro study, azithromycin appears to be an effective antibiotic comparable to tetracycline and erythromycin for use in the treatment of both C. trachomatis and C. pneumoniae infections. C1 JOHNS HOPKINS UNIV,DEPT MED,DIV INFECT DIS,BALTIMORE,MD 21205. NIAID,IMMUNOREGULAT LAB,BETHESDA,MD 20892. RI Gaydos, Charlotte/E-9937-2010 NR 18 TC 36 Z9 44 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD FEB PY 1992 VL 36 IS 2 BP 291 EP 294 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA HC450 UT WOS:A1992HC45000009 PM 1318677 ER PT J AU CORYELL, W ENDICOTT, J KELLER, M AF CORYELL, W ENDICOTT, J KELLER, M TI MAJOR DEPRESSION IN A NONCLINICAL SAMPLE - DEMOGRAPHIC AND CLINICAL RISK-FACTORS FOR 1ST ONSET SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID URBAN-RURAL DIFFERENCES; AFFECTIVE-DISORDER; PSYCHIATRIC-DISORDERS; UNITED-STATES; BIRTH-COHORT; RATES; PREVALENCE; SCHIZOPHRENIA; DEFINITION; COMMUNITY AB The relatives, controls, and spouses of affectively ill probands underwent diagnostic examinations on two occasions, 6 years apart. Of 965 subjects who had never been mentally ill when first examined, 11.8% had development of at least one episode of major depression as defined by the Research Diagnostic Criteria during the ensuing 6 years. Subjects younger than 40 years were three times more likely than older subjects to develop depression and women were approximately twice as likely as men to develop depression regardless of age. Marital disruption, a farm setting, and high educational achievement substantially increased the risk of depression among female subjects. Of 214 never-depressed subjects with a history of nonaffective mental disorder, 62 (29.0%) developed major depression. Age and sex were again powerful determinants. The course of prospectively observed secondary depression was more severe than that for primary depression. C1 NIMH,COLLABORAT PROGRAM PSYCHOBIOL DEPRESS CLIN STUDIES,BETHESDA,MD 20892. FU NIMH NIH HHS [R01 MH025478] NR 37 TC 92 Z9 92 U1 4 U2 6 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1992 VL 49 IS 2 BP 117 EP 125 PG 9 WC Psychiatry SC Psychiatry GA HC800 UT WOS:A1992HC80000004 PM 1550464 ER PT J AU CORYELL, W ENDICOTT, J KELLER, M AF CORYELL, W ENDICOTT, J KELLER, M TI RAPIDLY CYCLING AFFECTIVE-DISORDER - DEMOGRAPHICS, DIAGNOSIS, FAMILY HISTORY, AND COURSE SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID BIPOLAR AFFECTIVE-DISORDER; LITHIUM-CARBONATE; ILLNESS; IMIPRAMINE; RELIABILITY; UNIPOLAR; THERAPY AB Of 919 patients with major affective disorders who completed at least 1 year of a 5-year, semiannual follow-up, 45 developed a rapidly cycling bipolar course during the first year, but only one developed a rapidly cycling unipolar course. In comparison with patients who showed a non-rapidly cycling bipolar course, those who became rapid cyclers were more likely to be female and to have exhibited depression, hypomania, or cycling between depression and hypomania within the index episode. Family study data revealed no evidence that high cycle frequencies breed true. Rapid cycling was associated with a significantly lower likelihood of recovery in the second year of follow-up but not in the third, fourth, or fifth. These data suggest that rapid cycling is, in the large majority of cases, a transient, nonfamilial manifestation of bipolar affective disorder. C1 NIMH,COLLABORAT PROGRAM PSYCHOBIOL DEPRESS CLIN STUDIES,BETHESDA,MD 20892. FU NIMH NIH HHS [R01 MH025478] NR 25 TC 192 Z9 195 U1 2 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1992 VL 49 IS 2 BP 126 EP 131 PG 6 WC Psychiatry SC Psychiatry GA HC800 UT WOS:A1992HC80000005 PM 1550465 ER PT J AU JIMERSON, DC LESEM, MD KAYE, WH BREWERTON, TD AF JIMERSON, DC LESEM, MD KAYE, WH BREWERTON, TD TI LOW SEROTONIN AND DOPAMINE METABOLITE CONCENTRATIONS IN CEREBROSPINAL-FLUID FROM BULIMIC PATIENTS WITH FREQUENT BINGE EPISODES SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID NORMAL-WEIGHT BULIMIA; ANOREXIA-NERVOSA; EATING DISORDERS; PSYCHIATRIC-DISORDERS; HOMOVANILLIC-ACID; DOUBLE-BLIND; 3-METHOXY-4-HYDROXYPHENYLGLYCOL; BEHAVIOR; SUCROSE; SYSTEMS AB Cerebrospinal fluid neurotransmitter metabolite levels were studied to assess whether measures of central serotonin, dopamine, or norepinephrine function are associated with severity of abnormal eating patterns in patients with bulimia nervosa. In comparison with healthy controls (N = 17), hospitalized bulimic patients with a history of binge eating more frequently than twice daily (N = 11) had significantly lower CSF concentrations of 5-hydroxyindoleacetic acid and homovanillic acid. For the total patient group (N = 29), levels of both metabolites were significantly inversely correlated with binge frequency. On the basis of preclinical studies, these results were examined in the context of speculative models in which low central serotonin function might contribute to blunted satiety responses in bulimic patients, while low central dopamine activity might play a role in abnormal hedonic responses to food. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02115. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. RP JIMERSON, DC (reprint author), BETH ISRAEL HOSP,DEPT PSYCHIAT,330 BROOKLINE AVE,BOSTON,MA 02215, USA. NR 51 TC 164 Z9 166 U1 0 U2 9 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1992 VL 49 IS 2 BP 132 EP 138 PG 7 WC Psychiatry SC Psychiatry GA HC800 UT WOS:A1992HC80000006 PM 1372494 ER PT J AU UHL, GR PERSICO, AM SMITH, SS AF UHL, GR PERSICO, AM SMITH, SS TI CURRENT EXCITEMENT WITH D2 DOPAMINE RECEPTOR GENE ALLELES IN SUBSTANCE-ABUSE SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID ALCOHOLISM; ASSOCIATION C1 NIDA,ADDICT RES CTR,ETIOL BRANCH,BALTIMORE,MD 21224. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL & NEUROSCI,BALTIMORE,MD 21205. RP UHL, GR (reprint author), NIDA,ADDICT RES CTR,MOLEC NEUROBIOL LAB,BOX 5180,BALTIMORE,MD 21224, USA. NR 17 TC 67 Z9 67 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1992 VL 49 IS 2 BP 157 EP 160 PG 4 WC Psychiatry SC Psychiatry GA HC800 UT WOS:A1992HC80000009 PM 1347991 ER PT J AU SATO, Y BERKOWITZ, BA WILSON, CA DEJUAN, E AF SATO, Y BERKOWITZ, BA WILSON, CA DEJUAN, E TI BLOOD-RETINAL BARRIER BREAKDOWN CAUSED BY DIODE VS ARGON-LASER ENDOPHOTOCOAGULATION SO ARCHIVES OF OPHTHALMOLOGY LA English DT Article ID PROLIFERATIVE VITREORETINOPATHY; PHOTOCOAGULATION AB We compared the effects of argon and diode laser endophotocoagulation on blood-retinal barrier breakdown using real-time magnetic resonance imaging following intravenous gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) injection. Endophotocoagulation was performed on eyes of pigmented rabbits with either the argon or the diode laser to produce ophthalmoscopically similar lesions. Magnetic resonance imaging studies were performed either 2 or 7 days after laser treatment, and coronal T1-weighted proton images were obtained in the first 20 minutes following Gd-DTPA injection. The mean signal intensity over a region of interest in the vitreous cavity was analyzed, and an initial rate analysis was performed on each time-course curve. Two days after treatment, argon laser-treated eyes showed significantly greater leakage of Gd-DTPA than diode laser-treated eyes. The leakage in both groups was substantially reduced by posttreatment day 7. Histopathologic examination performed 2 days following photocoagulation showed less damage of the retinal pigment epithelium and more severe occlusion of the choriocapillaris and deep choroidal vessels in diode laser-treated eyes. These changes may serve to explain the observed differences in Gd-DTPA leakage. C1 DUKE UNIV,CTR EYE,DURHAM,NC 27706. NIEHS,RES TRIANGLE PK,NC 27709. FU NEI NIH HHS [R01 EY07576] NR 17 TC 45 Z9 48 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9950 J9 ARCH OPHTHALMOL-CHIC JI Arch. Ophthalmol. PD FEB PY 1992 VL 110 IS 2 BP 277 EP 281 PG 5 WC Ophthalmology SC Ophthalmology GA HD624 UT WOS:A1992HD62400035 PM 1736878 ER PT J AU CAMPOS, H GENEST, JJ BLIJLEVENS, E MCNAMARA, JR JENNER, JL ORDOVAS, JM WILSON, PWF SCHAEFER, EJ AF CAMPOS, H GENEST, JJ BLIJLEVENS, E MCNAMARA, JR JENNER, JL ORDOVAS, JM WILSON, PWF SCHAEFER, EJ TI LOW-DENSITY-LIPOPROTEIN PARTICLE-SIZE AND CORONARY-ARTERY DISEASE SO ARTERIOSCLEROSIS AND THROMBOSIS LA English DT Article DE LOW DENSITY LIPOPROTEIN PARTICLE SIZE; GRADIENT GEL ELECTROPHORESIS; PLASMA LIPOPROTEINS; TRIGLYCERIDES; CHOLESTEROL; APOLIPOPROTEINS; CORONARY ARTERY DISEASE ID APOLIPOPROTEIN-A-I; HEART-DISEASE; MYOCARDIAL-INFARCTION; CHOLESTEROL LEVELS; SUBCLASS PATTERNS; RISK-FACTORS; PLASMA; ATHEROSCLEROSIS; FRAMINGHAM; HETEROGENEITY AB Decreased plasma low density lipoprotein (LDL) particle size has been associated with premature coronary artery disease (CAD). We examined LDL particle size by 2-16% gradient gel electrophoresis in 275 men with CAD (> 75% cross-sectional-area stenosis) and 822 controls. Seven major LDL size bands (with LDL-1 [d = 1.025-1.033 g/ml] being the largest and LDL-7 [d = 1.050-1.063 g/ml, the smallest]) were identified. Because most subjects had two or more adjacent LDL bands, an LDL score was calculated for each subject, with the relative area in each band taken into consideration. Four major LDL particle size groups were classified in the present studies: large LDL, intermediate LDL, small LDL, and very small LDL. The use of beta-blockers was significantly associated with smaller LDL particles. After adjusting for use of this medication, small LDL particles were still more prevalent in CAD patients (39%) compared with controls (27%). The prevalence of large LDL particles was lower in CAD patients (3%) than in controls (24%). Intermediate LDL particles were the most prevalent in both groups, 49% in CAD patients and 46% in controls. The difference in LDL particle size between CAD patients and controls was not independent but was highly associated (p < 0.0001) with elevated triglyceride levels and decreased high density lipoprotein (HDL) cholesterol levels. Significantly higher LDL cholesterol levels were found in subjects with intermediate and small LDL particles than in those with large or very small LDL particles. In addition, CAD patients with intermediate or small LDL particles had significantly (p < 0.01) lower HDL cholesterol and apolipoprotein A-I levels and higher LDL cholesterol levels than did controls in the same group. Smoking, hypertension, diabetes, and HDL and LDL cholesterol levels were strong discriminators between CAD patients and controls, while triglycerides and LDL particle size did not add significant information to the model. These data indicate that small LDL particle size is not an independent discriminator for CAD after conventional risk factors and lipoprotein parameters such as LDL and HDL cholesterol have been taken into account. C1 TUFTS UNIV,USDA,HUMAN NUTR RES CTR AGING,LIPID METAB LAB,711 WASHINGTON ST,BOSTON,MA 02111. NEW ENGLAND MED CTR HOSP,DEPT MED,DIV CARDIOL,BOSTON,MA. NHLBI,FRAMINGHAM HEART STUDY,FRAMINGHAM,MA. OI Ordovas, Jose/0000-0002-7581-5680 FU NHLBI NIH HHS [HV83-03, HL-35243] NR 41 TC 434 Z9 439 U1 0 U2 6 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 1049-8834 J9 ARTERIOSCLER THROMB JI Arterioscler. Thromb. PD FEB PY 1992 VL 12 IS 2 BP 187 EP 195 PG 9 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HE668 UT WOS:A1992HE66800007 PM 1543692 ER PT J AU ANDERSON, DE CROWELL, MD FRENCH, AW AF ANDERSON, DE CROWELL, MD FRENCH, AW TI A TETHER SYSTEM FOR CARDIOVASCULAR STUDIES WITH THE BEHAVING MICROPIG SO BEHAVIOR RESEARCH METHODS INSTRUMENTS & COMPUTERS LA English DT Article ID BLOOD-PRESSURE; SWINE; PATTERNS AB A tether system has been developed for continuous monitoring of blood pressure in the micropig. The micropig is a suitable model for blood-pressure research because of the similarity of its cardiovascular system to that of humans and because of its sensitivity to high sodium intake. The system consists of a metal boom, attached via a universal joint to a wall 6 ft above floor level, that extends horizontally to the center of the enclosure. A fluid and electronic swivel affixed to the boom is connected to a flexible, hollow, metal tether that descends to a vest worn by the micropig. The vest contains a pressure transducer to which an indwelling arterial catheter is connected via a stopcock. The transducer cable and an infusion line ascend through the interior of the tether to the swivel. The system remains in equilibrium through a system of pulleys and counterweights. Continuous, 24-h recording shows a diurnal variation characterized by higher heart rate but lower blood pressure during the day than at night. The system has been found to be effective for continuous studies over intervals of a month or more. C1 JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21205. RP ANDERSON, DE (reprint author), NIA,GERONTOL RES CTR,BEHAV SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. OI Crowell, Michael/0000-0002-1162-3831 NR 12 TC 0 Z9 0 U1 0 U2 0 PU PSYCHONOMIC SOC INC PI AUSTIN PA 1710 FORTVIEW RD, AUSTIN, TX 78704 SN 0743-3808 J9 BEHAV RES METH INSTR JI Behav. Res. Methods Instr. Comput. PD FEB PY 1992 VL 24 IS 1 BP 44 EP 48 DI 10.3758/BF03203468 PG 5 WC Psychology, Mathematical; Psychology, Experimental SC Psychology GA HH569 UT WOS:A1992HH56900006 ER PT J AU OVERMAN, W BACHEVALIER, J TURNER, M PEUSTER, A AF OVERMAN, W BACHEVALIER, J TURNER, M PEUSTER, A TI OBJECT RECOGNITION VERSUS OBJECT DISCRIMINATION - COMPARISON BETWEEN HUMAN INFANTS AND INFANT MONKEYS SO BEHAVIORAL NEUROSCIENCE LA English DT Article ID INTERTRIAL INTERVALS; MEMORY; LESIONS AB Human infants (12-32 months old) and adults learned a delayed nonmatching-to-sample (DNMS) task and single- and multiple-pair discrimination tasks using nonverbal procedures previously used with monkeys. Infants learned discriminations rapidly and at a young age (12 months), but they required prolonged training and maturation before learning the DNMS task. Adults learned all tasks rapidly. After learning the DNMS task to criterion, memory performance declined systematically in an inverse relation to age. The dissociation in ability of infants on the DNMS versus discrimination tasks closely resembles the dissociation previously reported with infant monkeys (Bachevalier & Mishkin, 1984). Results from both infant humans and monkeys support a neurocognitive maturational model. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP OVERMAN, W (reprint author), UNIV N CAROLINA,DEPT PSYCHOL,WILMINGTON,NC 28403, USA. NR 37 TC 49 Z9 49 U1 0 U2 3 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0735-7044 J9 BEHAV NEUROSCI JI Behav. Neurosci. PD FEB PY 1992 VL 106 IS 1 BP 15 EP 29 DI 10.1037/0735-7044.106.1.15 PG 15 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA HF358 UT WOS:A1992HF35800002 PM 1554428 ER PT J AU GAFFAN, D MURRAY, EA AF GAFFAN, D MURRAY, EA TI MONKEYS (MACACA-FASCICULARIS) WITH RHINAL CORTEX ABLATIONS SUCCEED IN OBJECT DISCRIMINATION-LEARNING DESPITE 24-HR INTERTRIAL INTERVALS AND FAIL AT MATCHING TO SAMPLE DESPITE DOUBLE SAMPLE PRESENTATIONS SO BEHAVIORAL NEUROSCIENCE LA English DT Article ID SHORT-TERM-MEMORY; HIPPOCAMPAL-FORMATION; ENTORHINAL CORTEX; TEMPORAL CORTEX; LESIONS; AMYGDALA; FORNIX; RECOGNITION; IMPAIRMENT; TRANSECTION AB Six cynomolgus monkeys (Macaca fascicularis) learned preoperatively a set of 10 concurrent object discriminations with 24-hr intertrial intervals. Three then had the rhinal cortex removed bilaterally, whereas the other 3 remained as unoperated controls. The animals with ablations were impaired in reacquiring the preoperatively acquired set but subsequently learned without any impairment a new set of 10 discriminations that was presented in the same way. The monkeys with rhinal cortex ablations then failed to learn delayed matching to sample, with double sample presentations, in 510 trials, whereas the control animals learned this task in 270 trials on average. The results add to existing evidence that rhinal cortex ablation produces a severe impairment in visual short-term recognition memory and show for the first time that this impairment is accompanied by normal long-term discrimination learning ability. C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. RP GAFFAN, D (reprint author), UNIV OXFORD,DEPT EXPTL PSYCHOL,S PARKS RD,OXFORD OX1 3UD,ENGLAND. OI Murray, Elisabeth/0000-0003-1450-1642 NR 29 TC 227 Z9 228 U1 1 U2 8 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0735-7044 J9 BEHAV NEUROSCI JI Behav. Neurosci. PD FEB PY 1992 VL 106 IS 1 BP 30 EP 38 DI 10.1037/0735-7044.106.1.30 PG 9 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA HF358 UT WOS:A1992HF35800003 PM 1554436 ER PT J AU FLAMMANG, AM GELBOIN, HV AOYAMA, T GONZALEZ, FJ MCCOY, GD AF FLAMMANG, AM GELBOIN, HV AOYAMA, T GONZALEZ, FJ MCCOY, GD TI NICOTINE METABOLISM BY CDNA-EXPRESSED HUMAN CYTOCHROME-P-450S SO BIOCHEMICAL ARCHIVES LA English DT Article ID INDUCED RAT-LIVER; DIRECTED EXPRESSION; VACCINIA VIRUS; IN-VITRO; SEQUENCE; OXIDATION; GENE; IDENTIFICATION; PURIFICATION; MICROSOMES AB A cDNA-directed expression system in which individual human cytochrome P-450s are synthesized in cultured human hepatoma cells (HepG2) has been used to examine the individual P-450 forms for nicotine metabolism. Significant rates of nicotine C-oxidation were observed with seven of the twelve forms tested, with P-450 2B6 exhibiting the highest rate of nicotine metabolism. Forms 2C9 and 2E1 gave intermediate values and forms 2A6, 2C8, 2F1 and 4B1 exhibited trace levels of activity. No activity was detected with lysates containing forms IA2, 2D6, 3A3, 3A4, 3A5, or with non-transfected HepG2 cells. Nicotine N-oxide was not detected as a product with any of the P-450-containing cell lysates. Kinetic analysis of the 2B6 lysate demonstrated an apparent K(M) of 2.8 mM and a V(max) of 240 pmol/min/mg protein. The human P-450 2B6 tested herein appears to be the major nicotine metabolizing enzyme with the nicotine DELTA-1'(5')-iminium ion as the major product. The results presented indicate that the P-450 form specificity for the metabolism of nicotine has been conserved in the 2B subfamily across species. These studies further indicate that the HepG2 cell lysate cDNA-expression can provide a rapid and valuable system for use in determining in vitro metabolism of various toxic compounds. C1 NIH,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RP FLAMMANG, AM (reprint author), CASE WESTERN RESERVE UNIV,SCH MED,DEPT ENVIRONM HLTH SCI,CLEVELAND,OH 44106, USA. NR 37 TC 43 Z9 46 U1 1 U2 1 PU MBR PRESS INC PI KENYON PA PO BOX P, KENYON, MN 55946-000P SN 0749-5331 J9 BIOCHEM ARCH JI Biochem. Arch. PD FEB PY 1992 VL 8 IS 1 BP 1 EP 8 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA HB984 UT WOS:A1992HB98400001 ER PT J AU DRAKE, JW AF DRAKE, JW TI MUTATION-RATES SO BIOESSAYS LA English DT Article ID DNA-POLYMERASE; MUTAGENESIS RP DRAKE, JW (reprint author), NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709, USA. NR 21 TC 9 Z9 9 U1 1 U2 2 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD FEB PY 1992 VL 14 IS 2 BP 137 EP 140 DI 10.1002/bies.950140212 PG 4 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA HH949 UT WOS:A1992HH94900010 PM 1575714 ER PT J AU BARNETT, VA EHRLICH, A SCHOENBERG, M AF BARNETT, VA EHRLICH, A SCHOENBERG, M TI FORMATION OF ATP-INSENSITIVE WEAKLY-BINDING CROSSBRIDGES IN SINGLE-RABBIT PSOAS FIBERS BY TREATMENT WITH PHENYLMALEIMIDE OR PARA-PHENYLENEDIMALEIMIDE SO BIOPHYSICAL JOURNAL LA English DT Article ID SKELETAL-MUSCLE FIBERS; MYOSIN; ACTIN; NUCLEOTIDE; SUBFRAGMENT-1; TITIN AB Chaen et al. (1 986. J. Biol. Chem. 261:13632-13636) showed that treatment of relaxed single muscle fibers with para-phenylenedimaleimide (pPDM) results in inhibition of a fiber's ability to generate active force and a diminished ATPase activity. They postulated that the inhibition of force production was due to pPDM's ability to prevent crossbridges from participating in the normal ATP hydrolysis cycle. We find that the crossbridges produced by pPDM treatment of relaxed muscle cannot bind strongly to the actin filaments in rigor, but do bind weakly to the actin filaments in the presence and also absence of ATP. After pPDM treatment, fiber stiffness, as measured using ramp stretches of varying duration, is ATP-insensitive and identical to that of untreated relaxed fibers (both at high [165 mM] and low [40 mM] ionic strength). These results suggest that the pPDM-treated crossbridges, in both the presence and absence of ATP, are locked in a state that resembles the weakly-binding myosin . ATP state of normal crossbridges. Their resemblance to the ATP-crossbridges of relaxed untreated fibers is quite strong; both bind to actin about equally tightly and have similar attachment and detachment rate constants. We also found that crossbridges are locked in a weakly-binding state after treatment with N-phenylmaleimide (NPM). In muscle fibers, this method of producing weakly-binding crossbridges appears preferable to pPDM treatment because, unlike treatment with pPDM, it does not increase the fiber's resting tension and stiffness and it does not disrupt the titin band seen on SDS-PAGE. C1 NIAMSD,PHYS BIOL LAB,BETHESDA,MD 20892. NR 24 TC 14 Z9 14 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1992 VL 61 IS 2 BP 358 EP 367 PG 10 WC Biophysics SC Biophysics GA HC778 UT WOS:A1992HC77800007 PM 1547325 ER PT J AU HOROWITS, R AF HOROWITS, R TI PASSIVE FORCE GENERATION AND TITIN ISOFORMS IN MAMMALIAN SKELETAL-MUSCLE SO BIOPHYSICAL JOURNAL LA English DT Article ID THICK FILAMENT MOVEMENT; MONOCLONAL-ANTIBODIES; IMMUNOELECTRON MICROSCOPY; CONNECTIN FILAMENTS; POLYACRYLAMIDE GELS; SARCOMERE-LENGTH; ELASTIC PROTEIN; STRIATED-MUSCLE; MOLECULAR-SIZE; Z-LINE AB When relaxed striated muscle cells are stretched, a resting tension is produced which is thought to arise from stretching long, elastic filaments composed of titin (also called connectin). Here, I show that single skinned rabbit soleus muscle fibers produce resting tension that is several-fold lower than that found in rabbit psoas fibers. At sarcomere lengths where the slope of the resting tension-sarcomere length relation is low, electron microscopy of skinned fibers indicates that thick filaments move from the center to the side of the sarcomere during prolonged activation. As sarcomeres are stretched and the resting tension-sarcomere length relation becomes steeper, this movement is decreased. The sarcomere length range over which thick filament movement decreases is higher in soleus than in psoas fibers, paralleling the different lengths at which the slope of the resting tension-sarcomere length relations increase. These results indicate that the large differences in resting tension between single psoas and soleus fibers are due to different tensions exerted by the elastic elements linking the end of each thick filament to the nearest Z-disc, i.e., the titin filaments. Quantitative gel electrophoresis of proteins from single muscle fibers excludes the possibility that resting tension is less in soleus than in psoas fibers simply because they have fewer titin filaments. A small difference in the electrophoretic mobility of titin between psoas and soleus fibers suggests the alternate possibility that mammalian muscle cells use at least two titin isoforms with differing elastic properties to produce variations in resting tension. RP HOROWITS, R (reprint author), NIAMSD,BETHESDA,MD 20892, USA. NR 35 TC 107 Z9 108 U1 0 U2 9 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1992 VL 61 IS 2 BP 392 EP 398 PG 7 WC Biophysics SC Biophysics GA HC778 UT WOS:A1992HC77800010 PM 1547327 ER PT J AU ELION, J BERG, PE LAPOUMEROULIE, C TRABUCHET, G MITTELMAN, M KRISHNAMOORTHY, R SCHECHTER, AN LABIE, D AF ELION, J BERG, PE LAPOUMEROULIE, C TRABUCHET, G MITTELMAN, M KRISHNAMOORTHY, R SCHECHTER, AN LABIE, D TI DNA-SEQUENCE VARIATION IN A NEGATIVE CONTROL REGION 5' TO THE BETA-GLOBIN GENE CORRELATES WITH THE PHENOTYPIC-EXPRESSION OF THE BETA-S MUTATION SO BLOOD LA English DT Article ID SICKLE-CELL-ANEMIA; POLYMERASE CHAIN-REACTION; HEMOGLOBIN-S; ALPHA-THALASSEMIA; SOUTHERN INDIA; PROTEIN BINDS; DISEASE; RNA; HAPLOTYPES; ORIGIN C1 CHU COCHIN,PATHOL MOLEC LAB,F-75674 PARIS 14,FRANCE. UNIV LYON 1,CNRS,URM 106,BIOL CELLULAIRE LAB,F-69622 VILLEURBANNE,FRANCE. NIDDKD,CHEM BIOL LAB,BETHESDA,MD. RP ELION, J (reprint author), HOP ROBERT DEBRE,INSERM,U120,48 BD SERURIER,F-75019 PARIS,FRANCE. RI Elion, Jacques/A-1048-2014; OI Schechter, Alan N/0000-0002-5235-9408 NR 41 TC 83 Z9 83 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 1 PY 1992 VL 79 IS 3 BP 787 EP 792 PG 6 WC Hematology SC Hematology GA HB533 UT WOS:A1992HB53300033 PM 1346253 ER PT J AU SKRTIC, D EANES, ED AF SKRTIC, D EANES, ED TI MEMBRANE-MEDIATED PRECIPITATION OF CALCIUM-PHOSPHATE IN MODEL LIPOSOMES WITH MATRIX VESICLE-LIKE LIPID-COMPOSITION SO BONE AND MINERAL LA English DT Article DE CALCIUM PHOSPHATES; LIPOSOMES; MATRIX VESICLES; MINERALIZATION ID CONTAINING ANIONIC LIPOSOMES; EPIPHYSEAL CARTILAGE; AQUEOUS SUSPENSIONS; PYROPHOSPHATASE; ATPASE AB The present study examined calcium phosphate precipitation in aqueous suspensions of artificial liposomes which closely resembled matrix vesicles (MV) in membrane lipid composition. At 22-degrees-C, the liposomes per se did not initiate precipitation in the suspending medium for up to 120 h when the latter was made supersaturated with respect to hydroxyapatite (2.25 mM Ca2+, 1.5 mM PO4, 240 mosmol, pH 7.4). Likewise, the suspending medium remained stable for up to 72 h when precipitation was induced within the aqueous interiors of the liposomes by encapsulating pH 7.4-buffered 50 mM PO4 solutions in the interior spaces and making the enclosing membranes permeable to external solution Ca2+ ions with the ionophore X-537A. However, extraliposomal precipitation readily occurred under these latter conditions when phosphatidylserine (PS) and sphingomyelin (Sph) were deleted from the MV-like lipid formulation used to prepare the liposomes. These results suggest that lipidic membrane constituents such as PS and Sph may have a controlling influence on MV-mediated calcification in vivo by affecting the release of intravesicularly formed mineral crystals into the extracellular matrix space where they can subsequently grow and proliferate. C1 NATL INST STAND & TECHNOL,NIDR,RES ASSOCIATE PROGRAM,BONE RES BRANCH,BLDG 224,ROOM A143,GAITHERSBURG,MD 20899. NR 24 TC 23 Z9 23 U1 0 U2 4 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0169-6009 J9 BONE MINER JI Bone Miner. PD FEB PY 1992 VL 16 IS 2 BP 109 EP 119 DI 10.1016/0169-6009(92)90881-D PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HF847 UT WOS:A1992HF84700003 PM 1576486 ER PT J AU PASCUALLEONE, A BRASILNETO, JP VALLSSOLE, J COHEN, LG HALLETT, M AF PASCUALLEONE, A BRASILNETO, JP VALLSSOLE, J COHEN, LG HALLETT, M TI SIMPLE REACTION-TIME TO FOCAL TRANSCRANIAL MAGNETIC STIMULATION - COMPARISON WITH REACTION-TIME TO ACOUSTIC, VISUAL AND SOMATOSENSORY STIMULI SO BRAIN LA English DT Article ID MOTOR PROGRAMS; INTACT MAN; RESPONSES; MOVEMENT; MUSCLES AB We studied the effect of different go-signals on the reaction time in nine normal human subjects trained to respond by rapidly flexing one arm. Reaction times to auditory stimuli were shorter than those to visual or somatosensory stimuli, and were inversely correlated with the stimulus intensity. The reaction time was longest to a transcranial (magnetic or electric) stimulus delivered over the contralateral motor cortex that was sufficiently strong to induce a motor evoked potential in the responding biceps. Conversely, reaction time was shortest to either subthreshold transcranial stimulation over the same scalp position or to transcranial stimulation over the ipsilateral motor cortex regardless of intensity. Suprathreshold transcranial stimulation to the motor cortex seems to transiently inhibit the neurons responsible for initiation of motor programs involving muscles in which motor evoked potentials have been induced, thereby prolonging the reaction time. On the other hand, a subthreshold stimulus either disinhibits or directly activates such neurons leading to a shorter reaction time. Transcallosal connections between the motor cortices may account for the short reaction time to ipsilateral transcranial stimulation. C1 NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,HUMAN CORTICAL PHYSIOL UNIT,BLDG 10,BETHESDA,MD 20892. RI Pascual-Leone, Alvaro/G-6566-2011 NR 22 TC 71 Z9 73 U1 1 U2 4 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0006-8950 J9 BRAIN JI Brain PD FEB PY 1992 VL 115 BP 109 EP 122 DI 10.1093/brain/115.1.109 PN 1 PG 14 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA HQ077 UT WOS:A1992HQ07700007 PM 1559148 ER PT J AU ANGEL, I HAUGER, RL GIBLIN, BA PAUL, SM AF ANGEL, I HAUGER, RL GIBLIN, BA PAUL, SM TI REGULATION OF THE ANORECTIC DRUG RECOGNITION SITE DURING GLUCOPRIVIC FEEDING SO BRAIN RESEARCH BULLETIN LA English DT Article DE MAZINDOL BINDING SITE; NA+K+-ATPASE; GLUCORECEPTORS; GLUCOPRIVIC FEEDING; GENETIC OBESITY ID CENTRAL NERVOUS-SYSTEM; H-3 MAZINDOL BINDING; (+)-AMPHETAMINE BINDING; PARAVENTRICULAR NUCLEUS; GLUCOSTATIC REGULATION; FOOD-DEPRIVATION; BLOOD-GLUCOSE; OBESE MICE; RAT; BRAIN AB The acute effects of 2-deoxy-D-glucose (2-DG)-induced glucoprivic feeding on the anorectic drug recognition site and Na+K+-ATPase in the brain were examined in adult rats and in lean and genetically obese mice. The marked hyperglycemia and the induction of feeding caused by the administration of 2-DG to satiated rats and lean mice were associated with significant increases in Na+K+-ATPase activity, and in [H-3]ouabain binding and [H-3]mazindol binding to the anorectic drug recognition site in hypothalamic membranes. Basal and 2-DG-stimulated levels of blood glucose were significantly correlated to the levels of hypothalamic [H-3]ouabain (r = +.91, p < 0.01) and [H-3]mazindol (r = +.87, p < 0.01) binding. A significant correlation (r = .74, p < 0.05) was also observed between [H-3]mazindol binding and [H-3]ouabain binding supporting the hypothesis that these hypothalamic binding sites are functionally coupled in their response to circulating glucose. Following the intracerebroventricular (ICV) administration of the diabetogenic drug alloxan, 2-DG did not stimulate feeding or increase [H-3]mazindol and [H-3]ouabain binding sites in the hypothalamic paraventricular area. Since 2-DG still caused hyperglycemia in alloxan-treated rats, alloxan-induced inactivation of glucoreceptor mechanisms led to an uncoupling of the anorectic drug recognition site from a hypothalamic glucostat. In genetically obese mice (ob/ob), 2-DG also could not induce feeding or increase hypothalamic [H-3]ouabain or [H-3]mazindol binding, despite a significant hyperglycemic response. In contrast, 2-DG did increase feeding and the binding of [H-3]ouabain and [H-3]mazindol to the hypothalamus of lean littermates. The dissociation of the anorectic drug recognition site from blood glucose responses to 2-DG suggests that the glucoprivic feeding response in obese mice is impaired. In conclusion, the [H-3]mazindol recognition site and the neuronal Na+K+-ATPase labelled by [H-3]ouabain binding constitutes a glucoreceptive system which in response to changes in circulating glucose levels modulates feeding. Since the coupling of this anorectic drug recognition site to brain glucostats is defective in genetically obese mice, this system may have an important role in pathological hyperphagia and the development of obesity. C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20985. NR 50 TC 6 Z9 6 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0361-9230 J9 BRAIN RES BULL JI Brain Res. Bull. PD FEB PY 1992 VL 28 IS 2 BP 201 EP 207 DI 10.1016/0361-9230(92)90180-6 PG 7 WC Neurosciences SC Neurosciences & Neurology GA GZ884 UT WOS:A1992GZ88400008 PM 1317740 ER PT J AU GRANT, BF AF GRANT, BF TI PREVALENCE OF THE PROPOSED DSM-IV ALCOHOL-USE DISORDERS - UNITED-STATES, 1988 SO BRITISH JOURNAL OF ADDICTION LA English DT Article AB Data from a 1988 survey on United States drinking practices and related problems was used to derive the proposed DSM-IV definitions of alcohol abuse and dependence. The prevalence of DSM-IV alcohol abuse and dependence combined, incorporating the DSM-III-R duration criterion, was 6.00% in this general population sample. The majority of respondents were classified as alcohol dependent (5.93%), dependent without abuse (5.24%), and dependent with physiological dependence (5.06%). The rate for DSM-IV alcohol abuse was negligible (0.06%) while elimination of the duration criterion had little impact on the prevalence of DSM-IV alcohol use disorders. Reasons for the extremely low prevalence of DSM-IV alcohol abuse and the slight increase in the prevalence of DSM-IV alcohol use disorders as the result of eliminating the duration criterion are discussed in terms of the content of the abuse category and its relationship to the dependence definition. RP GRANT, BF (reprint author), NIAAA,DIV BIOMETRY & EPIDEMIOL,5600 FISHERS LANE,ROOM 14C-26,ROCKVILLE,MD 20857, USA. NR 4 TC 15 Z9 15 U1 0 U2 0 PU CARFAX PUBL CO PI ABINGDON PA PO BOX 25, ABINGDON, OXFORDSHIRE, ENGLAND OX14 3UE SN 0952-0481 J9 BRIT J ADDICT PD FEB PY 1992 VL 87 IS 2 BP 309 EP 316 PG 8 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA HE521 UT WOS:A1992HE52100018 PM 1555008 ER PT J AU PALLI, D BIANCHI, S DECARLI, A CIPRIANI, F AVELLINI, C COCCO, P FALCINI, F PUNTONI, R RUSSO, A VINDIGNI, C FRAUMENI, JF BLOT, WJ BUIATTI, E AF PALLI, D BIANCHI, S DECARLI, A CIPRIANI, F AVELLINI, C COCCO, P FALCINI, F PUNTONI, R RUSSO, A VINDIGNI, C FRAUMENI, JF BLOT, WJ BUIATTI, E TI A CASE-CONTROL STUDY OF CANCERS OF THE GASTRIC CARDIA IN ITALY SO BRITISH JOURNAL OF CANCER LA English DT Article ID DISTAL STOMACH; ADENOCARCINOMA; DIET; ESOPHAGUS AB In a case-control study of gastric cancer (GC) in high-risk and low-risk areas in Italy, 923 GCs were reviewed by one pathologist and classified according to anatomic site. There were 68 (7.4%) cancers occurring in the gastric cardia. Compared to other GCs, cardia cancer tended to occur more often in males (sex ratio 2.8 vs 1.7) and as intestinal or unclassified histologic types. Nutritional factors for cardia tumours resembled those of other GCs, showing inverse associations with the consumption of raw vegetables, citrus and other fresh fruit, and ascorbic acid, and positive associations with the intake of traditional soups and meat, protein and cholesterol, and preference for salty foods. Cigarette smoking and wine consumption were unrelated to cardia cancer risk, and there was only a weak association with total alcohol intake. Cardia tumours showed a greater familial occurrence of GC than did other sites, with a 7-fold increase in risk for those reporting two first-degree relatives with GC. The authors discuss these findings in view of the rising incidence of adenocarcinomas of the cardia and lower oesophagus that has been reported in some western countries. C1 UNIV FLORENCE,IST ANAT PATOL,I-50134 FLORENCE,ITALY. UNIV MILAN,IST STAT MED & BIOMETRIA,I-20133 MILAN,ITALY. IST NAZL TUMORI,I-20133 MILAN,ITALY. OSPED IMOLA,SERV ANAT PATOL,I-40026 IMOLA,ITALY. UNIV CAGLIARI,IST MED LAVORO,I-09124 CAGLIARI,ITALY. OSPED MORGAGNI PIERANTONI,SERV ONCOL,I-47100 FORLI,ITALY. IST NAZL RIC CANC,I-16132 GENOA,ITALY. UNIV SIENA,IST ANAT PATOL 2,I-53100 SIENNA,ITALY. NCI,BETHESDA,MD 20892. RP PALLI, D (reprint author), CTR STUDIO & PREVENZ ONCOL,UO EPIDEMIOL,USL 10E,VIALE VOLTA 171,I-50131 FLORENCE,ITALY. RI Decarli, Adriano/C-3129-2017; OI Decarli, Adriano/0000-0003-1451-8292; PALLI, Domenico/0000-0002-5558-2437; Russo, Antonio Giampiero/0000-0002-5681-5861 FU NCI NIH HHS [N01-CP-51019] NR 20 TC 85 Z9 85 U1 0 U2 1 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH, MIDLOTHIAN, SCOTLAND EH1 3AF SN 0007-0920 J9 BRIT J CANCER JI Br. J. Cancer PD FEB PY 1992 VL 65 IS 2 BP 263 EP 266 DI 10.1038/bjc.1992.52 PG 4 WC Oncology SC Oncology GA HE993 UT WOS:A1992HE99300024 PM 1739627 ER PT J AU GUPTA, MA GUPTA, AK ELLIS, CN VOORHEES, JJ AF GUPTA, MA GUPTA, AK ELLIS, CN VOORHEES, JJ TI BULIMIA-NERVOSA AND ACNE MAY BE RELATED - A CASE-REPORT SO CANADIAN JOURNAL OF PSYCHIATRY-REVUE CANADIENNE DE PSYCHIATRIE LA English DT Article ID EATING DISORDER INVENTORY; ANOREXIA-NERVOSA; HUMAN SKIN; WEIGHT AB Acne is a very common, often cosmetically disfiguring, cutaneous condition of adolescence that is associated with increased sebaceous gland activity. We present the case of a patient with bulimia who reported that the negative effect of acne on her appearance increased her body image concerns and exacerbated her eating disorder. Improvement of the acne was associated with a significant improvement in her eating disorder. Eating disordered patients may go on restrictive diets in order to control their acne since levels of androgens, which are one of the primary stimulants of sebaceous gland activity, are lower in starvation. As a significant number of adolescents with eating disorders also develop acne, it is important for the clinician to be aware of this previously unreported association between acne and eating disorders, and to evaluate the impact of acne upon the patient's body image and eating behaviour. C1 NIH,DERMATOL,BETHESDA,MD 20892. UNIV MICHIGAN,DERMATOL,ANN ARBOR,MI 48109. RP GUPTA, MA (reprint author), UNIV MICHIGAN,PSYCHIAT,ANN ARBOR,MI 48109, USA. NR 21 TC 10 Z9 10 U1 1 U2 3 PU CANADIAN PSYCHIATRIC ASSOC PI OTTAWA PA SUITE 200, 237 ARGYLE AVE, OTTAWA ON K2P 1B8, CANADA SN 0706-7437 J9 CAN J PSYCHIAT JI Can. J. Psychiat.-Rev. Can. Psychiat. PD FEB PY 1992 VL 37 IS 1 BP 58 EP & PG 0 WC Psychiatry SC Psychiatry GA HH782 UT WOS:A1992HH78200013 PM 1532340 ER PT J AU CANOBBIO, L BOCCARDO, F CANNATA, D GALLOTTI, P EPIS, R AF CANOBBIO, L BOCCARDO, F CANNATA, D GALLOTTI, P EPIS, R TI TREATMENT OF ADVANCED PANCREATIC-CARCINOMA WITH THE SOMATOSTATIN ANALOG BIM-23014 - PRELIMINARY-RESULTS OF A PILOT-STUDY SO CANCER LA English DT Article ID EPIDERMAL GROWTH-FACTOR; HORMONE; ADENOCARCINOMA; CELLS; TUMOR AB Treatment of advanced pancreatic cancer has not improved substantially in recent years. The search for new agents or new therapeutic modalities may be critical for further development in the therapy of this disease. Experimental and clinical findings suggest that it might be possible to develop a new hormonal therapy for exocrine cancer of the pancreas based on new somatostatin analogues. Preliminary results indicate clinical activity and increased survival in some patients. In this study, 19 patients with advanced exocrine pancreatic carcinoma were given the somatostatin analogue BIM 23014 using a range of doses from 250-mu-g/day to 1 mg/day. One patient had a partial response, 6 patients had stable disease, and 11 had progressive disease. Six patients showed a sharp improvement in pain and performance status, Side effects were mild. Plasma levels of growth hormone were evaluated in ten patients and remained unchanged. The clinical activity observed, even if limited, warrants further investigation using more appropriate schedules and administration techniques. C1 NATL CANC INST,GENOA,ITALY. GEN HOSP,VIGEVANO,ITALY. REG HOSP CORK,SONDALO,ITALY. NR 22 TC 36 Z9 36 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 1 PY 1992 VL 69 IS 3 BP 648 EP 650 DI 10.1002/1097-0142(19920201)69:3<648::AID-CNCR2820690308>3.0.CO;2-J PG 3 WC Oncology SC Oncology GA HB111 UT WOS:A1992HB11100007 PM 1346097 ER PT J AU BRYANT, GA AF BRYANT, GA TI THE PSYCHOLOGICAL-ASPECTS OF CHRONIC ILLNESS - GREGG,CH, ROBERTUS,JL, STONE,JB SO CANCER NURSING LA English DT Book Review RP BRYANT, GA (reprint author), NCI,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0162-220X J9 CANCER NURS JI Cancer Nurs. PD FEB PY 1992 VL 15 IS 1 BP 73 EP 74 PG 2 WC Oncology; Nursing SC Oncology; Nursing GA HF045 UT WOS:A1992HF04500013 ER PT J AU ADAMSON, PC BALIS, FM MISER, J ARNDT, C WELLS, RJ GILLESPIE, A ARONSON, L PENTA, JS CLENDENINN, NJ POPLACK, DG AF ADAMSON, PC BALIS, FM MISER, J ARNDT, C WELLS, RJ GILLESPIE, A ARONSON, L PENTA, JS CLENDENINN, NJ POPLACK, DG TI PEDIATRIC PHASE-I TRIAL, PHARMACOKINETIC STUDY, AND LIMITED SAMPLING STRATEGY FOR PIRITREXIM ADMINISTERED ON A LOW-DOSE, INTERMITTENT SCHEDULE SO CANCER RESEARCH LA English DT Article ID DIHYDROFOLATE-REDUCTASE; DRUG CONCENTRATION; INHIBITOR AB Piritrexim, an orally administered, lipid-soluble antifolate, was evaluated in a multi-institutional phase I trial in children. The starting dose was 10 mg/m2/dose administered every 8 h daily for 5 days for 3 consecutive weeks, with dose escalations in increments of 5 mg/m/dose. Eighteen patients (16 with metastatic sarcoma, 1 with acute lymphoblastic leukemia, and 1 with a brainstem glioma), 3.5-20 years of age, with malignancy refractory to therapy, were entered into the study. The dose-limiting toxicities (DLTs), which were myelosuppression and mucositis, occurred in 4 of 4 patients treated at the 25-mg/m2/dose level but in none of the patients treated at the 15- and 20-mg/m2/dose levels. The recommended dose for phase II trials is 20 mg/m2/dose. Pharmacokinetic monitoring was performed in 15 of the 18 children. The area under the concentration-time curve (AUC) was linearly related to the dose administered. Piritrexim was rapidly absorbed, with the median time to peak level occurring 1.5 h after an oral dose. The terminal half-life of piritrexim ranged from 1.5 to 4.5 h. A limited sampling strategy developed earlier, capable of predicting the AUC based on the plasma concentrations at 3 and 6 h after an oral dose, was prospectively tested in this trial and proved to be highly predictive of the AUC (r = 0.98, P = 0.0001). Pharmacodynamic-pharmacokinetic correlations were obtained after combining data from this and the prior phase I pediatric trial. Trough plasma piritrexim concentration strongly correlated with DLT (P = 0.0016). A trough plasma piritrexim concentration > 0.5-mu-M appeared to be predictive of toxicity. Eleven of 15 patients with trough concentrations exceeding this threshold experienced DLTs. Therapeutic drug monitoring may thus play an important role in adjusting the dose and schedule of piritrexim in future trials. C1 MAYO CLIN,ROCHESTER,MN 55902. CHILDRENS HOSP MED CTR,CINCINNATI,OH 45229. BURROUGHS WELLCOME CO,RES TRIANGLE PK,NC 27709. RP ADAMSON, PC (reprint author), NCI,PEDIAT BRANCH,BETHESDA,MD 20892, USA. NR 16 TC 25 Z9 25 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1992 VL 52 IS 3 BP 521 EP 524 PG 4 WC Oncology SC Oncology GA HB184 UT WOS:A1992HB18400004 PM 1732038 ER PT J AU COOK, JA GLASS, J LEBOVICS, R BOBO, H PASS, H DELANEY, TF OLDFIELD, EH MITCHELL, JB GLATSTEIN, E GOFFMAN, TE AF COOK, JA GLASS, J LEBOVICS, R BOBO, H PASS, H DELANEY, TF OLDFIELD, EH MITCHELL, JB GLATSTEIN, E GOFFMAN, TE TI MEASUREMENT OF THYMIDINE REPLACEMENT IN PATIENTS WITH HIGH-GRADE GLIOMAS, HEAD AND NECK TUMORS, AND HIGH-GRADE SARCOMAS AFTER CONTINUOUS INTRAVENOUS INFUSIONS OF 5-IODODEOXYURIDINE SO CANCER RESEARCH LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; UNRESECTABLE SARCOMAS; CLINICAL-EXPERIENCE; DNA; BROMODEOXYURIDINE; RADIOSENSITIZERS; IODODEOXYURIDINE; TIME AB Based upon the radiation sensitization properties of the halogenated pyrimidines, 5-iododeoxyuridine (IdUrd) and 5-bromodeoxyuridine, long term i.v. infusions of halogenated pyrimidines in conjunction with fractionated radiation therapy have been evaluated in the treatment of a variety of human malignancies. While clinical studies have attempted to measure the halogenated pyrimidine incorporation, few have successfully related tumor response to the incorporation of IdUrd by the tumor. The present study reports the continuous IdUrd labeling index (number of cells labeled) and the IdUrd corrected replacement (percentage of thymidine replacement in the labeled cells of the population) from the tumors of 17 patients who received continuous infusions of IdUrd (1000 mg/m2/24 h). The tumors treated included four high grade gliomas, five head and neck tumors, four high grade sarcomas, and five other tumors of varying types. Less than 25% of the cells in three of four gliomas incorporated IdUrd after 5-7-day IdUrd infusion time. Corrected replacement for the gliomas ranged from 0 to 4%. In contrast, 63-85% of the cells in the head and neck biopsies were labeled with IdUrd after 3-7-day IdUrd infusions suggesting that these large tumors (3-12 cm diameter) have a high fraction of dividing cells. Corrected replacements values for the head and neck tumor patients ranged from 2.9 to 26.3%. The high grade sarcomas also demonstrated a high percentage of IdUrd labeled cells (57-79%) with three patients having corrected replacements of 7.5-14.2%. The continuous labeling and thymidine replacement data for four patients from whom serial biopsies were taken during IdUrd infusion demonstrated both an increasing IdUrd replacement and continuous labeling index with an increasing duration of IdUrd infusion. The clinical response of both the high grade glioma and head and neck tumor patients indicate that the IdUrd replacement and labeling data may provide some important predictive information with regard to the successful use of the halogenated pyrimidines in clinical radiation trials. C1 NCI,RADIAT ONCOL BRANCH,BLDG 10,ROOM B3-B69,BETHESDA,MD 20892. NCI,SURG NEUROL BRANCH,BETHESDA,MD 20892. NCI,THORAC SURG BRANCH,BETHESDA,MD 20892. NID,OTOLARYNGOL BRANCH,BETHESDA,MD 20892. NR 23 TC 34 Z9 34 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1992 VL 52 IS 3 BP 719 EP 725 PG 7 WC Oncology SC Oncology GA HB184 UT WOS:A1992HB18400033 PM 1732059 ER PT J AU DATTA, AK MISRA, M NORTH, SL KASPRZAK, KS AF DATTA, AK MISRA, M NORTH, SL KASPRZAK, KS TI ENHANCEMENT BY NICKEL(II) AND L-HISTIDINE OF 2'-DEOXYGUANOSINE OXIDATION WITH HYDROGEN-PEROXIDE SO CARCINOGENESIS LA English DT Article ID CELL-NUCLEI; DNA; SUPEROXIDE; RATS; RADICALS; INVITRO; BINDING; NI-63 AB Conversion of the 2'-deoxyguanosine (dG) residues in calf thymus DNA to 8-hydroxy-2'-deoxyguanosine (8-OH-dG) was achieved at physiological pH by treating the DNA with hydrogen peroxide (H2O2) in the presence of nickel(II) chloride (NiCl2). The effectiveness of this reaction was enhanced by L-histidine (His) which forms the Ni(II)-His2 complex. Similar effects of NiCl2 and His were observed on hydroxylation of pure dG with H2O2. The rate of pure dG conversion to 8-OH-dG at 37-degrees-C (100 mM phosphate buffer) depended on combination and concentration of the reagents and on pH. Following 24 h incubation at pH 7.4 of 0.75 mM dG with 30 mM H2O2 and 1 mM NiCl2, dG was converted into 8-OH-dG to the extent of 0.05% in the absence of His and 0.45% in the presence of 2 mM His. After 24 h incubation at pH 7.4 of 0.5 mg/ml DNA with 7.5 mM H2O2 and 0.1 mM NiCl2, 0.18% of the dG moiety was converted into 8-OH-dG in the absence of His and 0.42% in the presence of 0.2 mM His. Interestingly, a mixture of H2O2 with His was also capable of oxidizing dG to 8-OH-dG even in the absence of NiCl2, albeit less effectively than in the presence of NiCl2. This effect was not suppressed after treatment of dG, His and the buffer with Chelex to remove divalent metal contaminants, if any. The exact chemistry of the observed phenomena remains to be determined. Since the Ni(II)-His2 complex is the major low mol. wt nickel carrier in mammalian organisms, the observed redox properties of this complex, reported here for the first time, may be crucial for the toxicity and carcinogenicity of nickel. RP DATTA, AK (reprint author), NCI, FREDERICK CANC RES & DEV CTR, COMPARAT CARCINOGENESIS LAB, FREDERICK, MD 21702 USA. NR 25 TC 50 Z9 51 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0143-3334 EI 1460-2180 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1992 VL 13 IS 2 BP 283 EP 287 DI 10.1093/carcin/13.2.283 PG 5 WC Oncology SC Oncology GA HE543 UT WOS:A1992HE54300024 ER PT J AU EBERHART, J COFFING, SL ANDERSON, JN MARCUS, C KALOGERIS, TJ BAIRD, WM PARK, SS GELBOIN, HV AF EBERHART, J COFFING, SL ANDERSON, JN MARCUS, C KALOGERIS, TJ BAIRD, WM PARK, SS GELBOIN, HV TI THE TIME-DEPENDENT INCREASE IN THE BINDING OF BENZO[A]PYRENE TO DNA THROUGH (+)-ANTI-BENZO[A]PYRENE-7,8-DIOL-9,10-EPOXIDE IN PRIMARY RAT HEPATOCYTE CULTURES RESULTS FROM INDUCTION OF CYTOCHROME P450IA1 BY BENZO[A]PYRENE TREATMENT SO CARCINOGENESIS LA English DT Note ID EMBRYO CELL-CULTURES; MONOCLONAL-ANTIBODIES; METABOLIC-ACTIVATION; MUTAGEN ACTIVATION; BENZO(A)PYRENE; 7,12-DIMETHYLBENZ(A)ANTHRACENE; 7,12-DIMETHYLBENZANTHRACENE; PROTEINS; TISSUES AB The proportion and amount of benzo[a]pyrene (B[a]P) that binds to DNA through the carcinogenic (+)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide [(+)-anti-BPDE] increases with time of exposure to B[a]P in cell cultures derived from a number of species. Pretreatment of primary rat hepatocyte cultures for 12 h with 1-mu-g B[a]P/ml medium increased the subsequent metabolism of [H-3]B[a]P by 47% and [H-3]B[a]P-DNA binding by 53% compared with acetone-pretreated hepatocytes. The amount of (+)-anti-BPDE bound to DNA in the B[a]P-pretreated hepatocytes increased 175%. B[a]P pretreatment also increased DNA-binding 2-fold in hepatocytes treated with [H-3]7,8-dihydroxy-7,8-dihydro-B[a]P but had no effect on DNA binding in cells treated with anti-B[a]P-7,8-diol-9,10-epoxide. Western blotting showed that cytochrome P450IA1, which was not detectable prior to B[a]P treatment, was selectively increased by B[a]P treatment. A monoclonal antibody that specifically inhibits cytochrome P450IA1 reduced the binding of B[a]P to DNA by > 90% in microsomal preparations from B[a]P-pretreated hepatocytes. These results indicate that the time-dependent increase in the formation of (+)-anti-BPDE-DNA adducts results from an increase in the amount and proportion of B[a]P metabolized to this ultimate carcinogen by P450IA1 that is induced by the B[a]P treatment. The importance of P450IA1 induction by the B[a]P for its activation to this ultimate carcinogenic metabolite suggests that long-term exposure of cells to B[a]P could result in activation of a higher proportion of the B[a]P to the carcinogenic (+)-anti-BPDE. C1 PURDUE UNIV,DEPT MED CHEM & PHARMACOGNOSY,W LAFAYETTE,IN 47907. PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907. PURDUE UNIV,DEPT PHARMACOL & TOXICOL,W LAFAYETTE,IN 47907. PURDUE UNIV,DEPT FOOD & NUTR,W LAFAYETTE,IN 47907. NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. FU NCI NIH HHS [CA40228]; NIEHS NIH HHS [ES05322] NR 32 TC 24 Z9 27 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1992 VL 13 IS 2 BP 297 EP 301 DI 10.1093/carcin/13.2.297 PG 5 WC Oncology SC Oncology GA HE543 UT WOS:A1992HE54300026 PM 1740021 ER PT J AU FOWLIS, DJ FLANDERS, KC DUFFIE, E BALMAIN, A AKHURST, RJ AF FOWLIS, DJ FLANDERS, KC DUFFIE, E BALMAIN, A AKHURST, RJ TI DISCORDANT TRANSFORMING GROWTH FACTOR-BETA-1 RNA AND PROTEIN LOCALIZATION DURING CHEMICAL CARCINOGENESIS OF THE SKIN SO CELL GROWTH & DIFFERENTIATION LA English DT Article ID BRONCHIAL EPITHELIAL-CELLS; FACTOR TYPE-BETA; TGF-BETA; MOUSE SKIN; TERMINAL DIFFERENTIATION; REVERSIBLE INHIBITION; CDNA CLONING; KERATINOCYTES; EXPRESSION; RESISTANT AB Transforming growth factor-beta (TGF-beta) inhibits proliferation of normal keratinocytes, and this response is retained, to variable extents, in benign tumors of the skin (S. Haddow, D. J. Fowlis, K. Parkinson, R. J. Akhurst, and A. Balmain, Oncogene, 6: 1465-1470, 1991). To investigate the profile of TGF-beta biosynthesis during various stages of chemical carcinogenesis of the skin, we used a combination of ribonuclease protection assay, in situ hybridization with gene-specific probes for TGF-beta-1, -beta-2, and -beta-3, and immunohistochemistry with isoform-specific antibodies against TGF-beta-1. Following 12-O-tetradecanoylphorbol-13-acetate treatment of adult mouse skin, there was a rapid induction of TGF-beta-1 protein. Intracellular TGF-beta-1 protein was localized to suprabasal keratinocytes, and the extracellular form was localized predominantly to the dermis. Despite ubiquitous induction of TGF-1 protein by 12-O-tetradecanoylphorbol-13-acetate in various mouse strains, we noted strain-specific differences in the quantitative induction of TGF-beta-1 RNA. Papillomas and carcinomas induced in vivo had elevated levels of TGF-beta-1 RNA within the basal keratinocyte compartment but did not contain significant levels of TGF-beta-1 protein within the tumor. We postulate that the tumor evades TGF-beta-1-controlled negative growth regulation by altered translational and/or posttranslational processing mechanisms of this growth factor. Levels of TGF-beta-2 and -beta-3 RNA were not elevated it any stage of chemical carcinogenesis of the skin. C1 UNIV GLASGOW,YORKHILL HOSP,DUNCAN GUTHRIE INST MED GENET,GLASGOW G3 8SJ,SCOTLAND. NIH,CHEMOPREVENT LAB,BETHESDA,MD 20892. WOLFSON LAB MOLEC PATHOL,BEATSON LABS,CANC RES CAMPAIGN,BEARSDEN G61 1BD,ENGLAND. FU Wellcome Trust NR 64 TC 64 Z9 64 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 1044-9523 J9 CELL GROWTH DIFFER JI Cell Growth Differ. PD FEB PY 1992 VL 3 IS 2 BP 81 EP 91 PG 11 WC Cell Biology SC Cell Biology GA HC982 UT WOS:A1992HC98200001 PM 1504019 ER PT J AU KOVACS, G KIECHLESCHWARZ, M SCHERER, G KUNG, HF AF KOVACS, G KIECHLESCHWARZ, M SCHERER, G KUNG, HF TI MOLECULAR ANALYSIS OF THE CHROMOSOME-11P REGION IN RENAL-CELL CARCINOMAS SO CELLULAR AND MOLECULAR BIOLOGY LA English DT Article DE RENAL CELL TUMOR; RFLP ANALYSIS; CHROMOSOME-11P REGION ID WILMS-TUMOR; PAPILLARY; DELETION; 11P13 AB Comparative histological data suggest that papillary renal cell tumors in adults and Wilms' tumors in children develop from maturation-arrested cells of similar origin. Wilms' tumor is characterized by genetic changes at the chromosome 11p region. In the present study, we have analyzed 10 papillary and 10 non-papillary renal cell tumors and determined the allelic status of 6 loci on the short arm of chromosome 11. Only one papillary renal cell carcinoma among the 20 tumors showed a loss of constitutional heterozygosity for the chromosome 11p region. These data suggest that separate molecular events occur in the development of Wilms' tumor and papillary renal cell tumors, subsequent to the proliferation of maturation-arrested cells of the kidney. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. UNIV FREIBURG,DEPT HUMAN GENET,W-7800 FREIBURG,GERMANY. RP KOVACS, G (reprint author), NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 13 TC 3 Z9 3 U1 0 U2 0 PU CELLULAR & MOLECULAR BIOLOGY PI NOISY-LE-GRAND PA PROF R WEGMANN RESIDENCE HAUSSMANN 1 AVENUE DU PAVE NEUF, 93160 NOISY-LE-GRAND, FRANCE SN 0145-5680 J9 CELL MOL BIOL JI Cell. Mol. Biol. PD FEB PY 1992 VL 38 IS 1 BP 59 EP 62 PG 4 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HE448 UT WOS:A1992HE44800007 PM 1348450 ER PT J AU STEIN, PD ATHANASOULIS, C ALVAI, A GREENSPAN, RH HALES, CA SALTZMAN, HA VREIM, CE TERRIN, ML WEG, JG AF STEIN, PD ATHANASOULIS, C ALVAI, A GREENSPAN, RH HALES, CA SALTZMAN, HA VREIM, CE TERRIN, ML WEG, JG TI COMPLICATIONS AND VALIDITY OF PULMONARY ANGIOGRAPHY IN ACUTE PULMONARY-EMBOLISM SO CIRCULATION LA English DT Article DE ANGIOGRAPHY; ARTERIOGRAPHY; CATHETERIZATION; PULMONARY EMBOLISM ID CARDIAC ANGIOGRAPHY; CONTRAST AGENT; CINEANGIOGRAPHY; TRIAL AB Background. The Prospective Investigation of Pulmonary Embolism Diagnosis (PIOPED) addressed the value of ventilation/perfusion scans in acute pulmonary embolism (PE). The present study evaluates the risks and diagnostic validity of pulmonary angiography in 1,111 patients who underwent angiography in PIOPED. Methods and Results. Complications were death in five (0.5%), major nonfatal complications in nine (1%), and less significant or minor in 60 (5%). More fatal or major nonfatal complications occurred in patients from the medical intensive care unit than elsewhere: five of 122 (4%) versus nine of 989 (1%) (p < 0.02). Pulmonary artery pressure, volume of contrast material, and presence of PE did not significantly affect the frequency of complications. Renal dysfunction, either major (requiring dialysis) or less severe, occurred in 13 of 1,111 (1%). Patients who developed renal dysfunction after angiography were older than those who did not have renal dysfunction: 74 +/- 13 years versus 57 +/- 17 years (p < 0.001). Angiograms were nondiagnostic in 35 of 1,111 (3%), and studies were incomplete in 12 of 1,111 (1%), usually because of a complication. Surveillance after negative angiograms showed PE in four of 675 (0.6%). Angiograms, interpreted on the basis of consensus readings, resulted in an unchallenged diagnosis in 96%. Conclusions. The risks of pulmonary angiography were sufficiently low to justify it as a diagnostic tool in the appropriate clinical setting. Clinical judgment is probably the most important consideration in the assessment of risk. C1 MARYLAND MED RES INST,BALTIMORE,MD. DUKE UNIV,DURHAM,NC 27706. YALE UNIV,NEW HAVEN,CT 06520. UNIV PENN,PHILADELPHIA,PA 19104. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. UNIV MICHIGAN,ANN ARBOR,MI 48109. NIH,BETHESDA,MD 20892. RP STEIN, PD (reprint author), HENRY FORD HEART & VASC INST,2799 W GRAND BLVD,DETROIT,MI 48202, USA. FU NHLBI NIH HHS [N01-HR-34007, N01-HR-34008, N01-HR-34009] NR 21 TC 488 Z9 501 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1992 VL 85 IS 2 BP 462 EP 468 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HC293 UT WOS:A1992HC29300005 PM 1735144 ER PT J AU WILLIAMS, DO BRAUNWALD, E KNATTERUD, G BABB, J BRESNAHAN, J GREENBERG, MA RAIZNER, A WASSERMAN, A ROBERTSON, T ROSS, R THOMPSON, B BELL, WR SCHERLIS, L DODGE, HT BROWN, BG KENNEDY, JW SHEEHAN, FH BISSON, B BOLSON, E ZARET, B WACKERS, F KAYDEN, DS DAVIS, K GREEN, R MANN, K STUMP, D COLLEN, D BOVILL, E TRACY, R ROSS, AM BREN, GB WASSERMAN, AG CHAITMAN, BR WIENS, RD SHAW, L HAUEISEN, M YOUNIS, LT PASSAMANI, ER ROBERTSON, TL LAN, G SOLOMON, R SOPKO, G ROBERTS, WC KALAN, J WILLIAMS, DO RILEY, R WHITE, H SHARAF, B FEDELE, F THOMAS, E DREW, T JOELSON, J HARDINK, D COLLING, C HAAKENSON, C SATHER, M BRAUNWALD, E KNATTERUD, GL TERRIN, ML FORMAN, S HARRIS, DT ROSS, R WILKINS, PC FREDERICK, M CANNER, PL CARROLL, M DEPKIN, J DOTSON, J FIERY, C JOHNSON, M KELLY, C NOBLE, P AF WILLIAMS, DO BRAUNWALD, E KNATTERUD, G BABB, J BRESNAHAN, J GREENBERG, MA RAIZNER, A WASSERMAN, A ROBERTSON, T ROSS, R THOMPSON, B BELL, WR SCHERLIS, L DODGE, HT BROWN, BG KENNEDY, JW SHEEHAN, FH BISSON, B BOLSON, E ZARET, B WACKERS, F KAYDEN, DS DAVIS, K GREEN, R MANN, K STUMP, D COLLEN, D BOVILL, E TRACY, R ROSS, AM BREN, GB WASSERMAN, AG CHAITMAN, BR WIENS, RD SHAW, L HAUEISEN, M YOUNIS, LT PASSAMANI, ER ROBERTSON, TL LAN, G SOLOMON, R SOPKO, G ROBERTS, WC KALAN, J WILLIAMS, DO RILEY, R WHITE, H SHARAF, B FEDELE, F THOMAS, E DREW, T JOELSON, J HARDINK, D COLLING, C HAAKENSON, C SATHER, M BRAUNWALD, E KNATTERUD, GL TERRIN, ML FORMAN, S HARRIS, DT ROSS, R WILKINS, PC FREDERICK, M CANNER, PL CARROLL, M DEPKIN, J DOTSON, J FIERY, C JOHNSON, M KELLY, C NOBLE, P TI ONE-YEAR RESULTS OF THE THROMBOLYSIS IN MYOCARDIAL-INFARCTION INVESTIGATION (TIMI) PHASE II TRIAL SO CIRCULATION LA English DT Article DE ACUTE MYOCARDIAL INFARCTION; THROMBOLYSIS; RT-PA; CORONARY ANGIOPLASTY; CORONARY ARTERY DISEASE; CARDIAC CATHETERIZATION ID CORONARY-BYPASS SURGERY; FOLLOW-UP; ACUTE CLOSURE; ANGIOPLASTY; REPERFUSION; SURVIVAL AB Background. The Thrombolysis in Myocardial Infarction (TIMI) Phase II Trial randomized 3,339 patients to either an invasive (INV, n = 1,681) or a conservative (CON, n = 1,658) strategy after intravenous recombinant tissue-type plasminogen activator (rt-PA) for acute myocardial infarction. Methods and Results. The patients assigned to the INV strategy routinely underwent cardiac catheterization, and when anatomically appropriate, percutaneous transluminal coronary angioplasty (PTCA) or coronary artery bypass grafting 18-48 hours after infarction. CON patients had these procedures only in response to the occurrence of spontaneous or provoked ischemia. One-year follow-up data are available in 3,316 patients (99.3%). The primary trial end point, death and nonfatal reinfarction, occurred in 14.7% of INV patients and in 15.2% of CON patients (p = NS). When analyzed individually, there was no difference (p = NS) in death (INV, 6.9%; CON, 7.4%) or recurrent infarction (INV, 9.4%; CON, 9.8%) between the two groups. Anginal status at 1 year was also similar. Cardiac catheterization and PTCA were performed more often in INV (98.0% and 61.2%, respectively) compared with CON (45.2% and 20.5%, respectively) patients. At 1 year, the cumulative number of patients who underwent coronary bypass surgery (INV, 17.5%; CON, 17.3%) was similar in the two groups. Conclusions. The INV and CON strategies resulted in similar favorable outcomes at 1 year of follow-up. In particular, the rates of mortality and reinfarction were not different and were impressively low in both groups. One possible advantage of the INV strategy was detected in subgroup analyses. In patients with a history of myocardial infarction, the data are suggestive that 1-year mortality was lower in INV patients (10.3%) than in CON patients (17.0%) (p = 0.03). C1 HARVARD UNIV,CAMBRIDGE,MA 02138. MARYLAND MED RES INST,CTR COORDINATING,BALTIMORE,MD. UNIV WASHINGTON,RADIOG CORE LAB,SEATTLE,WA 98195. YALE UNIV,RADIONUCLIDE CORE LAB,NEW HAVEN,CT 06520. UNIV VERMONT,COAGULAT CORE LAB,BURLINGTON,VT 05405. GEORGE WASHINGTON UNIV,ELECTROCARDIOG CORE LAB QUALIFYING CARDIOGRAMS,WASHINGTON,DC. ST LOUIS UNIV,ELECTROCARDIOG CORE LAB EXERCISE ELECTROCARDIOGRAMS,ST LOUIS,MO 63103. NHLBI,PROGRAM OFF,BETHESDA,MD 20892. NIH,CTR CLIN,PATHOL CORE LAB,BETHESDA,MD 20892. BROWN UNIV,PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTY QUAL CONTROL LAB,PROVIDENCE,RI 02912. VET ADM MED RES SERV,COOPERAT STUDIES PROGRAM,ALBUQUERQUE,NM. RP WILLIAMS, DO (reprint author), BROWN UNIV,RHODE ISL HOSP,DEPT MED,DIV CARDIOL,PROGRAM MED,593 EDDY ST,PROVIDENCE,RI 02903, USA. NR 22 TC 108 Z9 111 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1992 VL 85 IS 2 BP 533 EP 542 PG 10 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HC293 UT WOS:A1992HC29300012 PM 1735149 ER PT J AU DILSIZIAN, V FREEDMAN, NMT BACHARACH, SL PERRONEFILARDI, P BONOW, RO AF DILSIZIAN, V FREEDMAN, NMT BACHARACH, SL PERRONEFILARDI, P BONOW, RO TI REGIONAL THALLIUM UPTAKE IN IRREVERSIBLE DEFECTS - MAGNITUDE OF CHANGE IN THALLIUM ACTIVITY AFTER REINJECTION DISTINGUISHES VIABLE FROM NONVIABLE MYOCARDIUM SO CIRCULATION LA English DT Article DE CORONARY ARTERY DISEASE; MYOCARDIAL ISCHEMIA; MYOCARDIAL VIABILITY; THALLIUM SCINTIGRAPHY; POSITRON EMISSION TOMOGRAPHY ID PERSISTENT DEFECTS; TL-201 REINJECTION; METABOLIC-ACTIVITY; WALL MOTION; REDISTRIBUTION; SPECT; REVASCULARIZATION; PERFUSION; ISCHEMIA AB Background. Thallium reinjection immediately after stress-redistribution imaging identifies ischemic but viable myocardium in as many as 50% of the regions characterized by conventional redistribution imaging as irreversibly injured. However, we have previously shown that some regions in which irreversible defects persist despite reinjection are metabolically active, and hence viable, by positron emission tomography. In the current study, we determined whether the severity of reduction in thallium activity within irreversible defects on redistribution images and the magnitude of change in regional thallium activity after reinjection can further discriminate viable from nonviable myocardium in such defects. Methods and Results. We studied 150 patients with coronary artery disease by exercise thallium tomography using the rest-reinjection protocol. The three sets of images (stress, redistribution, and reinjection) were then analyzed quantitatively. The increase in regional thallium activity from redistribution to reinjection was computed, normalized to the increase observed in a normal region, and termed "differential uptake." Of the 175 myocardial regions designated to have irreversible thallium defects on conventional 3-4-hour redistribution images, 132 had only mild-to-moderate reduction in thallium activity (51-85% of normal activity), and 43 had severe reduction in thallium activity (less-than-or-equal-to 50% of normal activity). Thallium reinjection resulted in enhanced relative activity in 60 of 132 (45%) of the mild-to-moderate irreversible defects and 22 of 43 (51%) of the severe irreversible defects, leaving roughly half of these defects remaining irreversible after reinjection. However, in regions that appeared to remain irreversible despite reinjection, the magnitude of differential uptake differed between mild-to-moderate (74 +/- 14%) and severe (35 +/- 16%) irreversible defects (p < 0.001). All regions with mild-to-moderate defects demonstrated > 50% differential uptake after reinjection. In contrast, all except two of the regions with severe irreversible defects demonstrated differential uptake of < 50%. Fifteen patients also underwent positron emission tomography at rest with F-18-fluorodeoxyglucose (FDG) and O-15-water. FDG uptake was present in 91% of regions with mild-to-moderate reduction in thallium activity, and the results of differential uptake and FDG data were concordant in 81% of these regions. Conclusions. These data indicate that the magnitude of thallium uptake after reinjection differs between mild-to-moderate and severe irreversible defects on standard 3-4-hour redistribution images. The substantial differential uptake of thallium (> 50%) after reinjection in mild-to-moderate defects, even when relative thallium activity does not increase appreciably (and the defect appears to remain irreversible), coupled with preserved metabolic activity by positron emission tomography, supports the concept that such mild-to-moderate irreversible defects represent viable myocardium. C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. RP DILSIZIAN, V (reprint author), NIH,DEPT NUCL MED,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. NR 13 TC 109 Z9 109 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1992 VL 85 IS 2 BP 627 EP 634 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HC293 UT WOS:A1992HC29300022 PM 1735157 ER PT J AU EPSTEIN, ND FANANAPAZIR, L LIN, HJ MULVIHILL, J WHITE, R LALOUEL, JM LIFTON, RP NIENHUIS, AW LEPPERT, M AF EPSTEIN, ND FANANAPAZIR, L LIN, HJ MULVIHILL, J WHITE, R LALOUEL, JM LIFTON, RP NIENHUIS, AW LEPPERT, M TI EVIDENCE OF GENETIC-HETEROGENEITY IN 5 KINDREDS WITH FAMILIAL HYPERTROPHIC CARDIOMYOPATHY SO CIRCULATION LA English DT Article DE HYPERTROPHIC CARDIOMYOPATHY; LINKAGE ANALYSIS; GENETIC HETEROGENEITY ID HEAVY-CHAIN GENE; TWO-DIMENSIONAL ECHOCARDIOGRAPHY; M-MODE; CLINICAL MANIFESTATIONS; PATHO-PHYSIOLOGY; CYSTIC-FIBROSIS; MOLECULAR-BASIS; DNA; INHERITANCE; CHROMOSOME-2 AB Background. Recently, two families with hypertrophic cardiomyopathy have been shown to have mutations in the cardiac beta-myosin heavy chain gene (beta-MHC) located on the long arm of chromosome 14. Methods and Results. We have performed linkage analysis of five newly ascertained pedigrees with more than 50 chromosomal markers detecting polymorphisms. Our findings confirm the linkage to beta-MHC gene locus on chromosome 14 in one family (LOD score, 4.50) and suggest linkage to the same gene in another kindred. Chromosome 14 markers were not linked to the disease gene in the other three kindreds, however, and a test for genetic heterogeneity was statistically significant. Moreover, markers for the beta-MHC gene identified affected individuals who were recombinants with respect to this gene and the disease phenotype in these three kindreds. Conclusions. These results provide conclusive evidence that hypertrophic cardiomyopathy in separate families is caused by mutations in disease genes at two or more locations in the genome. C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. UNIV UTAH,SCH MED,DEPT HUMAN GENET,SALT LAKE CITY,UT 84112. HOWARD HUGHES MED INST,COCONUT GROVE,FL 33133. RP EPSTEIN, ND (reprint author), NHLBI,CLIN HEMATOL BRANCH,ROOM 7C-103,BLDG 10,BETHESDA,MD 20892, USA. NR 41 TC 65 Z9 68 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1992 VL 85 IS 2 BP 635 EP 647 PG 13 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HC293 UT WOS:A1992HC29300023 PM 1735158 ER PT J AU GARG, R WAGENER, DK MADANS, JH AF GARG, R WAGENER, DK MADANS, JH TI ALCOHOL-CONSUMPTION AND RISK OF ISCHEMIC-HEART-DISEASE IN WOMEN SO CIRCULATION LA English DT Meeting Abstract C1 NHLBI,DECA,CLIN TRIALS BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1992 VL 85 IS 2 BP 875 EP 875 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA HC293 UT WOS:A1992HC29300102 ER PT J AU FLUGELMAN, MY VIRMANI, R LEON, MB BOWMAN, RL DICHEK, DA AF FLUGELMAN, MY VIRMANI, R LEON, MB BOWMAN, RL DICHEK, DA TI GENETICALLY ENGINEERED ENDOTHELIAL-CELLS REMAIN ADHERENT AND VIABLE AFTER STENT DEPLOYMENT AND EXPOSURE TO FLOW INVITRO SO CIRCULATION RESEARCH LA English DT Article DE GENE THERAPY; BETA-GALACTOSIDASE; RETROVIRAL VECTORS; INTRAVASCULAR PROSTHESIS ID RETROVIRAL VECTORS; VASCULAR STENTS; GENE-TRANSFER; EXPRESSION AB Intravascular stents, currently in experimental human use for recurrent arterial stenosis, are plagued by subacute thrombosis. As a therapeutic approach to stent-related thrombosis, we and others have suggested coating stents with endothelial cells before implantation. In a previous study we demonstrated the feasibility of coating stents with endothelial cells that were genetically modified to secrete large amounts of human tissue plasminogen activator. In the present study we attempted both to develop a clinically applicable protocol for stent seeding and to test whether seeded cells would remain adherent to stents after exposure to pulsatile flow. Endothelial cells were harvested from the saphenous veins of sheep with survival of the donor animals. Harvested cells were transduced with a retroviral vector containing a marker gene and seeded onto catheter-mounted stents under sterile conditions. Scanning electron microscopy revealed complete coverage of the stent surfaces by seeded cells. Stents were expanded and exposed to pulsatile flow in vitro. Substantial cell retention was observed on the lateral stent surfaces by light microscopy and scanning electron microscopy; fewer cells were seen on the luminal and abluminal surfaces. Removal of seeded cells from How-exposed stents by trypsin digestion resulted in the recovery of approximately 70% of the seeded cells. These cells were viable and healthy as judged by their ability to proliferate to confluence with the same kinetics as control (non-flow-exposed) cells. Autologous genetically modified endothelial cells can be seeded onto catheter-mounted stents in a sterile manner, and stent deployment under flow conditions results in substantial retention of viable cells. C1 NHLBI,MOLEC HEMATOL BRANCH,BLDG 10,ROOM 7D-18,BETHESDA,MD 20892. NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. ARMED FORCES INST PATHOL,WASHINGTON,DC 20306. NR 21 TC 86 Z9 91 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD FEB PY 1992 VL 70 IS 2 BP 348 EP 354 PG 7 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA HC554 UT WOS:A1992HC55400014 PM 1735134 ER PT J AU REIDENBERG, MM FLACK, MR PYLE, RG MULLENS, NM LORENZO, B WU, YW KNAZEK, RA NISULA, BC AF REIDENBERG, MM FLACK, MR PYLE, RG MULLENS, NM LORENZO, B WU, YW KNAZEK, RA NISULA, BC TI GOSSYPOL TREATMENT OF METASTATIC ADRENAL CANCER SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 CORNELL UNIV,MED CTR,COLL MED,DEPT PHARMACOL,NEW YORK,NY 10021. CORNELL UNIV,MED CTR,COLL MED,DEPT MED,NEW YORK,NY 10021. NICHHD,DEV ENDOCRINE BRANCH,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 145 EP 145 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300096 ER PT J AU ZEIGLER, D BENJAMIN, J CRAIG, BE LI, SH SHOAF, SE MAX, MB AF ZEIGLER, D BENJAMIN, J CRAIG, BE LI, SH SHOAF, SE MAX, MB TI SINGLE DOSES OF DESIPRAMINE DO NOT POTENTIATE POSTOPERATIVE MORPHINE ANALGESIA SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIDR,NEUROBIOL & ANESTHESIA BRANCH,BETHESDA,MD 20892. NIAAA,CLIN STUDIES LAB,BETHESDA,MD. WALTER REED ARMY MED CTR,WASHINGTON,DC 20307. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 146 EP 146 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300102 ER PT J AU JOHNSON, RE FUDALA, PJ CHESKIN, LR AF JOHNSON, RE FUDALA, PJ CHESKIN, LR TI A COMPARISON OF BUPRENORPHINE TO CLONIDINE FOR RAPID INPATIENT OPIATE DETOXIFICATION SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. NIDA,ADDICT RES CTR,LEXINGTON,KY 40583. UNIV PENN,PHILADELPHIA,PA 19104. NR 0 TC 2 Z9 2 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 167 EP 167 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300185 ER PT J AU GEORGE, DT ALIM, T LINDQUIST, T LINNOILA, M AF GEORGE, DT ALIM, T LINDQUIST, T LINNOILA, M TI DIETARY-EFFECTS OF 2-DG IN ALCOHOLICS AND CONTROLS SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIAAA,CLIN SCI LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 168 EP 168 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300189 ER PT J AU PICKWORTH, WB BUNKER, EB GEORGE, F KLEIN, S SNIDOW, N HENNINGFIELD, JE AF PICKWORTH, WB BUNKER, EB GEORGE, F KLEIN, S SNIDOW, N HENNINGFIELD, JE TI ETHANOL (E) INTERACTIONS WITH PROSTAGLANDIN SYNTHESIS INHIBITORS (PGSI) IN HUMANS SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIDA,ADDICT RES CTR,BALTIMORE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 168 EP 168 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300186 ER PT J AU STEVENS, RC LAIZURE, SC SANDERS, PL STEIN, DS AF STEVENS, RC LAIZURE, SC SANDERS, PL STEIN, DS TI PHARMACOKINETICS OF TRIMETHOPRIM-SULFAMETHOXAZOLE (TMP-SMX) IN HEALTHY-SUBJECTS USING A REDUCED-PNEUMOCYSTIS-CARINII PNEUMONIA (PCP) DOSING REGIMEN SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 UNIV TENNESSEE CTR HLTH SCI,MEMPHIS,TN 38163. NIAID,DIV AIDS,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 182 EP 182 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300243 ER PT J AU IRWIN, RP DAWKINS, K FLEISHAKER, JC POTTER, WZ AF IRWIN, RP DAWKINS, K FLEISHAKER, JC POTTER, WZ TI NEUROENDOCRINE EFFECTS OF INTRAVENOUS ADINAZOLAM AND N-DESMETHYL-ADINAZOLAM ADMINISTRATION IN MAN SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIMH,BETHESDA,MD 20892. UPJOHN CO,KALAMAZOO,MI 49001. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 187 EP 187 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300265 ER PT J AU CHEN, G HOUGH, C MANJI, H CHUANG, D POTTER, WZ AF CHEN, G HOUGH, C MANJI, H CHUANG, D POTTER, WZ TI DESIPRAMINE AND CARBAMAZEPINE MODULATE-BETA ADRENERGIC-RECEPTORS (BETA-ARS) SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIMH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 190 EP 190 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300274 ER PT J AU SONCRANT, TT ASTHANA, S GREIG, NH SHETTY, HU DALY, E RAFFAELE, KC BERARDI, A MORRIS, PP SCHAPIRO, MB AF SONCRANT, TT ASTHANA, S GREIG, NH SHETTY, HU DALY, E RAFFAELE, KC BERARDI, A MORRIS, PP SCHAPIRO, MB TI KINETICS AND DYNAMICS OF THE CHOLINERGIC AGONIST ARECOLINE IN ALZHEIMERS-DISEASE SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIA,NEUROSCI LAB,PHARMACOL & PHARMACOKINET UNIT,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1992 VL 51 IS 2 BP 194 EP 194 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA HE703 UT WOS:A1992HE70300289 ER PT J AU CLARK, KF FRANKLIN, PS REISCH, JS HOFFMAN, HJ GAHL, WA THOENE, JG SCHNEIDER, JA AF CLARK, KF FRANKLIN, PS REISCH, JS HOFFMAN, HJ GAHL, WA THOENE, JG SCHNEIDER, JA TI EFFECT OF CYSTEAMINE-HCL AND PHOSPHOCYSTEAMINE DOSAGE ON RENAL-FUNCTION AND GROWTH IN CHILDREN WITH NEPHROPATHIC CYSTINOSIS SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF SAN DIEGO,LA JOLLA,CA 92093. UNIV TEXAS,HLTH SCI CTR,DALLAS,TX 75235. NICHHD,BETHESDA,MD. NR 0 TC 5 Z9 5 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A113 EP A113 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300625 ER PT J AU FRIEDMAN, D KNOX, JD SIADATPAJOUH, M KITTELSON, J NAVRE, M BOWDEN, T NAGLE, RB STETLERSTEVENSON, W AF FRIEDMAN, D KNOX, JD SIADATPAJOUH, M KITTELSON, J NAVRE, M BOWDEN, T NAGLE, RB STETLERSTEVENSON, W TI COLLAGENASE EXPRESSION IN NORMAL PROSTATE, PIN, AND INVASIVE PROSTATIC-CARCINOMA SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV ARIZONA,DEPT SURG,TUCSON,AZ 85721. UNIV ARIZONA,DEPT RADIAT ONCOL,TUCSON,AZ 85721. UNIV ARIZONA,DEPT PATHOL,TUCSON,AZ 85721. NCI,PATHOL LAB,BALTIMORE,MD 21211. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A63 EP A63 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300344 ER PT J AU HAO, W SERREZE, DV LEITER, EH KUFF, EL MCCULLOCH, DK NEIFING, JL PALMER, JP AF HAO, W SERREZE, DV LEITER, EH KUFF, EL MCCULLOCH, DK NEIFING, JL PALMER, JP TI INSULIN (AUTO)ANTIBODIES FROM HUMAN IDDM CROSS-REACT WITH RETROVIRAL ANTIGEN-P73 SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. JACKSON LAB,BAR HARBOR,ME 04609. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A35 EP A35 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300187 ER PT J AU ROBBINS, JH GANGES, MB BARRETT, SF TARONE, RE AF ROBBINS, JH GANGES, MB BARRETT, SF TARONE, RE TI LACK OF CORRELATION BETWEEN SEVERITY OF DNA-REPAIR DEFECT IN SKIN FIBROBLASTS AND NEUROLOGICAL STATUS IN XERODERMA-PIGMENTOSUM SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A124 EP A124 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300682 ER PT J AU ROSE, SR NUNEZ, SB AF ROSE, SR NUNEZ, SB TI INSULIN-LIKE GROWTH FACTOR-I AND INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN-3 COMPARED WITH STIMULATED AND OVERNIGHT GROWTH-HORMONE IN SHORT CHILDREN SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV NEW MEXICO,ALBUQUERQUE,NM 87131. NICHHD,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A25 EP A25 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300136 ER PT J AU ZILBERSTEIN, M MALOZOWSKI, S PARMER, TG TROJAN, S GIBORI, G ROBERTS, CL LEROITH, D WERNER, H MERRIAM, GR AF ZILBERSTEIN, M MALOZOWSKI, S PARMER, TG TROJAN, S GIBORI, G ROBERTS, CL LEROITH, D WERNER, H MERRIAM, GR TI GROWTH-HORMONE MODULATES INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA IN RAT OVARY SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV WASHINGTON,SEATTLE,WA 98195. VET ADM MED CTR,TACOMA,WA. NIH,BETHESDA,MD 20892. US FDA,BETHESDA,MD 20014. UNIV ILLINOIS,CHICAGO,IL 60680. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1992 VL 40 IS 1 BP A49 EP A49 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HA103 UT WOS:A1992HA10300264 ER PT J AU THOMAS, DG GART, JJ AF THOMAS, DG GART, JJ TI IMPROVED AND EXTENDED EXACT AND ASYMPTOTIC METHODS FOR THE COMBINATION OF 2X2 TABLES SO COMPUTERS AND BIOMEDICAL RESEARCH LA English DT Article ID COMMON ODDS RATIO; ESTIMATORS; INTERVAL; SPARSE; RISK RP THOMAS, DG (reprint author), NCI,BIOSTAT BRANCH,BETHESDA,MD 20892, USA. NR 22 TC 32 Z9 32 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0010-4809 J9 COMPUT BIOMED RES JI Comput. Biomed. Res. PD FEB PY 1992 VL 25 IS 1 BP 75 EP 84 DI 10.1016/0010-4809(92)90036-A PG 10 WC Computer Science, Interdisciplinary Applications; Medical Informatics SC Computer Science; Medical Informatics GA HC032 UT WOS:A1992HC03200006 PM 1547628 ER PT J AU GAIL, MH BYAR, DP PECHACEK, TF CORLE, DK AF GAIL, MH BYAR, DP PECHACEK, TF CORLE, DK TI ASPECTS OF STATISTICAL DESIGN FOR THE COMMUNITY INTERVENTION TRIAL FOR SMOKING CESSATION (COMMIT) SO CONTROLLED CLINICAL TRIALS LA English DT Article DE GROUP RANDOMIZATION; CLUSTER RANDOMIZATION; MATCHING; SMOKING PREVALENCE; COMMUNITY STUDIES ID RANDOMIZATION; CLUSTER; HEALTH; EDUCATION; MORTALITY; CAMPAIGN; PROJECT; QUIT AB We present statistical considerations for the design of the Community Intervention Trial for Smoking Cessation (COMMIT). One outcome measurement, the quit rate in randomly selected cohorts of smokers, is compared with another outcome measurement, the decrease in smoking prevalence, in terms of statistical efficiency and interpretability. The COMMIT study uses both types of outcome measurements. The merits of pair-matching the communities are considered, and sample size calculations take into account heterogeneity among pair-matched communities. In addition to significance tests based on the premutational (randomization) distribution, we also describe approaches for covariate adjustment. The COMMIT design includes 11 pair-matched communities, which should provide good power to detect a 10% or greater difference in quit rates between the intervention and control communities in cohorts of heavy smokers and in cohorts of light or moderate smokers. The power is only moderate to detect intervention effects on the decreases in overall smoking prevalence or in the prevalence of heavy smoking. C1 NCI,DIV CANC PREVENT & CONTROL,ROCKVILLE,MD. RP GAIL, MH (reprint author), NCI,DIV CANC ETIOL,6130 EXECUT BLVD,EPN 403,ROCKVILLE,MD 20892, USA. NR 39 TC 85 Z9 86 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0197-2456 J9 CONTROL CLIN TRIALS JI Controlled Clin. Trials PD FEB PY 1992 VL 13 IS 1 BP 6 EP 21 DI 10.1016/0197-2456(92)90026-V PG 16 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA HN337 UT WOS:A1992HN33700002 PM 1315664 ER PT J AU PERKINS, LL CUTTER, GR WAGENKNECHT, LE SAVAGE, PJ DYER, AR BIRCH, R AF PERKINS, LL CUTTER, GR WAGENKNECHT, LE SAVAGE, PJ DYER, AR BIRCH, R TI DISTRIBUTED DATA-ANALYSIS IN A MULTICENTER STUDY - THE CARDIA STUDY SO CONTROLLED CLINICAL TRIALS LA English DT Article DE DISTRIBUTED DATA ANALYSIS; MULTICENTER STUDIES; COORDINATING CENTERS ID YOUNG-ADULTS; BLOOD-PRESSURE; INSULIN AB Unlike distributed data entry, which is used in many large epidemiologic studies and multicenter clinical trials, distributed data analysis is a relatively new concept. This paper reports on the usefulness of such a system in the Coronary Artery Risk Development in Young Adults (CARDIA) Study. CARDIA distributes the entire examination dataset to participating centers soon after completion of each round of data collection. The process was designed to encourage more numerous, diverse, and rapid publications, and to allow for more efficient use of the manpower and expertise in centers. Responsibilities of the coordinating center have changed from a conventional coordinating center but remain substantial due to the need for collating, monitoring, verifying, and documenting the distributed data analysis (DDA) system. DDA is successful from the standpoint of implementation and operation-21 manuscripts representing work analyzed at six participating centers had been submitted for publication within 3.5 years of the completion of the baseline examination. C1 ST JUDE CHILDRENS RES HOSP, DIV BIOSTAT & INFORMAT SYST, MEMPHIS, TN 38101 USA. WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DEPT PUBL HLTH SCI, WINSTON SALEM, NC 27103 USA. NHLBI, BETHESDA, MD 20892 USA. NORTHWESTERN UNIV, SCH MED, CHICAGO, IL 60611 USA. BIOL THERAPY INST, FRANKLIN, TN USA. RP PERKINS, LL (reprint author), UNIV ALABAMA, SCH PUBL HLTH, 212 TIDWELL HALL, BIRMINGHAM, AL 35294 USA. FU NHLBI NIH HHS [N01-HC-48047, N01-HC-48049, N01-HC-48048] NR 22 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-2456 J9 CONTROL CLIN TRIALS JI Controlled Clin. Trials PD FEB PY 1992 VL 13 IS 1 BP 80 EP 90 DI 10.1016/0197-2456(92)90031-T PG 11 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA HN337 UT WOS:A1992HN33700007 PM 1315666 ER PT J AU KUTTY, G HAYDEN, B OSAWA, Y WIGGERT, B CHADER, GJ KUTTY, RK AF KUTTY, G HAYDEN, B OSAWA, Y WIGGERT, B CHADER, GJ KUTTY, RK TI HEME OXYGENASE - EXPRESSION IN HUMAN RETINA AND MODULATION BY STRESS AGENTS IN A HUMAN RETINOBLASTOMA CELL MODEL SYSTEM SO CURRENT EYE RESEARCH LA English DT Article ID BILIVERDIN REDUCTASE; HEPATOMA-CELLS; HEAT-SHOCK; RAT-LIVER; PURIFICATION; RETINOPATHY; PREMATURITY; BILIRUBIN; CDNA; GENE AB PCR and Southern blot analyses demonstrate that mRNA for heme oxygenase (HO), a well known "stress protein" in a number of tissues, is present in human retina. Western and northern blots show that the protein and mRNA are also expressed in human Y-79 retinoblastoma cells in culture and that the HO enzyme is rapidly induced by its substrate, heme. Moreover, HO is also induced by two chemicals, sodium arsenite and menadione, that act as agents of oxidative stress. HO is the regulatory enzyme in the heme degradative pathway and an increase in its activity could lead to the accumulation of bilirubin, an antioxidant, in the cell at the expense of heme, a prooxidant. The HO pathway may thus be of importance in protecting the retina against oxidative stress in vivo. Moreover, the Y-79 culture system should provide an excellent model for use in examining stress mechanisms in retinal cells at a molecular level. C1 NHLBI,DRUG TISSUE INTERACT SECT,CHEM PHARMACOL SECT,BETHESDA,MD 20892. NHLBI,PHARMACOL CHEM SECT,BETHESDA,MD 20892. RP KUTTY, G (reprint author), NEI,RETINAL CELL & MOLEC BIOL,BLDG 6,ROOM 218,BETHESDA,MD 20892, USA. NR 34 TC 20 Z9 20 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0271-3683 J9 CURR EYE RES JI Curr. Eye Res. PD FEB PY 1992 VL 11 IS 2 BP 153 EP 160 DI 10.3109/02713689209000066 PG 8 WC Ophthalmology SC Ophthalmology GA HJ856 UT WOS:A1992HJ85600006 PM 1572205 ER PT J AU SHAUGHNESSY, M WISTOW, G AF SHAUGHNESSY, M WISTOW, G TI ABSENCE OF MHC GENE-EXPRESSION IN LENS AND CLONING OF DBPB/YB-1, A DNA-BINDING PROTEIN EXPRESSED IN MOUSE LENS SO CURRENT EYE RESEARCH LA English DT Article ID T-HELPER LYMPHOCYTE; NORMAL HUMAN ORGANS; DETAILED DISTRIBUTION; CRYSTALLIN GENE; COLD SHOCK; Y-BOX; SEQUENCE; SPECIFICITY; PROMOTER; ANTIGEN AB The status of major histocompatibility complex (MHC) class I and II gene expression in the normal mouse lens was examined. No mRNA for either class I or II genes was detectable in mouse lens, while the expression of MHC genes in other tissues generally matched immunohistochemical data from human tissues. However it was observed that MHC class I mRNA is present in the mouse lens-derived cell line alpha-TN4-1. From a new-born mouse lens cDNA library a clone was obtained for the murine homologue of the DNA-binding protein dbpB/YB-1, a protein originally identified in human lymphocytes and proposed to be a negative regulator of MHC class II gene expression. Northern blots detect dbpB/YB-1 mRNA in all mouse tissues and cells examined, including both mouse lens and alpha-TN4-1 cells, suggesting that dbpB/YB-1 has a general and widespread role. C1 NEI,MOLEC & DEV BIOL LAB,MOLEC STRUCT & FUNCT SECT,ROOM 204,BLDG 6,BETHESDA,MD 20892. NR 32 TC 22 Z9 22 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0271-3683 J9 CURR EYE RES JI Curr. Eye Res. PD FEB PY 1992 VL 11 IS 2 BP 175 EP 181 DI 10.3109/02713689209000068 PG 7 WC Ophthalmology SC Ophthalmology GA HJ856 UT WOS:A1992HJ85600008 PM 1572207 ER PT J AU KINET, JP AF KINET, JP TI THE GAMMA-ZETA DIMERS OF FC-RECEPTORS AS CONNECTORS TO SIGNAL TRANSDUCTION SO CURRENT OPINION IN IMMUNOLOGY LA English DT Review ID MAST-CELL RECEPTOR; NATURAL-KILLER-CELLS; AFFINITY IGE RECEPTOR; ANTIGEN RECEPTOR; IMMUNOGLOBULIN-E; TRANSFECTED CELLS; MOLECULAR-CLONING; SURFACE EXPRESSION; ETA-CHAIN; SUBUNIT AB The gamma-chain of the high affinity receptor for IgE and the zeta-chain of the T-cell antigen receptor associate with various receptors. These chains play a major role in assembly of these receptors and are critical for signal transduction. RP KINET, JP (reprint author), NIAID,MOLEC ALLERGY & IMMUNOL SECT,ROCKVILLE,MD 20852, USA. NR 47 TC 66 Z9 67 U1 0 U2 1 PU CURRENT BIOLOGY LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0952-7915 J9 CURR OPIN IMMUNOL JI Curr. Opin. Immunol. PD FEB PY 1992 VL 4 IS 1 BP 43 EP 48 DI 10.1016/0952-7915(92)90122-U PG 6 WC Immunology SC Immunology GA HZ103 UT WOS:A1992HZ10300009 PM 1534484 ER PT J AU GALLIN, JI LETO, TL ROTROSEN, D KWONG, CH MALECH, HL AF GALLIN, JI LETO, TL ROTROSEN, D KWONG, CH MALECH, HL TI DELINEATION OF THE PHAGOCYTE NADPH OXIDASE THROUGH STUDIES OF CHRONIC GRANULOMATOUS DISEASES OF CHILDHOOD SO CURRENT OPINION IN IMMUNOLOGY LA English DT Review ID NEUTROPHIL CYTOCHROME-B; RESPIRATORY BURST; CHAIN; CLONING; GENE; EXPRESSION; COMPONENT; FORMS AB The phagocyte NADPH oxidase is a complex system consisting of membrane and cytosolic components that must assemble at the membrane for proper activation. Studies of patients with chronic granulomatous diseases of childhood have enabled the molecular characterization of these components, which has led to studies defining their interaction during NADPH complex assembly. Understanding NADPH oxidase assembly provides an opportunity to develop therapeutics for the regulation of this important reaction of inflammation. RP GALLIN, JI (reprint author), NIAID,HOST DEF LAB,BETHESDA,MD 20892, USA. NR 27 TC 13 Z9 13 U1 0 U2 0 PU CURRENT BIOLOGY LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0952-7915 J9 CURR OPIN IMMUNOL JI Curr. Opin. Immunol. PD FEB PY 1992 VL 4 IS 1 BP 53 EP 56 DI 10.1016/0952-7915(92)90124-W PG 4 WC Immunology SC Immunology GA HZ103 UT WOS:A1992HZ10300011 PM 1317712 ER PT J AU SINGER, DE NATHAN, DM ANDERSON, KM WILSON, PWF EVANS, JC AF SINGER, DE NATHAN, DM ANDERSON, KM WILSON, PWF EVANS, JC TI ASSOCIATION OF HBA1C WITH PREVALENT CARDIOVASCULAR-DISEASE IN THE ORIGINAL COHORT OF THE FRAMINGHAM-HEART-STUDY SO DIABETES LA English DT Article ID HIGH-DENSITY-LIPOPROTEIN; GLYCOSYLATED HEMOGLOBIN; GLUCOSE-TOLERANCE; RISK-FACTORS; INSULIN RESISTANCE; DIABETES-MELLITUS; RETINOPATHY; INDIVIDUALS; PLASMA; ATHEROSCLEROSIS AB We studied the cross-sectional relationship between HbA1c and cardiovascular disease (CVD) in the survivors of the original cohort of the Framingham Heart Study (n = 1045). HbA1c was significantly related to prevalent CVD among women but not men. HbA1c was also related to hypertension and to the ratio of total to high-density lipoprotein cholesterol levels. In regression analyses that controlled for these and other potential risk factors, HbAc remained significantly related to CVD among women. The relative odds of CVD increased 1.39-fold (95% confidence interval 1.06-1.83) for increases in HbA1c of 1% (e.g., for HbA1c from 5 to 6%). The relationship was not weakened when known diabetic subjects or subjects taking 13-blocker or thiazide medications were excluded from analysis. In contrast, there was no significant relationship between "casual" blood glucose and prevalent CVD. Our results reveal a strong, significant, independent association between hyperglycemia, measured by HbA1c, and CVD among older women. C1 MASSACHUSETTS GEN HOSP,DEPT MED,DIABET UNIT,BOSTON,MA 02114. NHLBI,FRAMINGHAM HEART STUDY,FRAMINGHAM,MA. HARVARD UNIV,SCH MED,DEPT HLTH CARE POLICY,BOSTON,MA 02115. BOSTON UNIV HOSP,MED CTR,SCH MED,EVANS MEM DEPT CLIN RES,PREVENT MED & EPIDEMIOL SECT,BOSTON,MA 02218. RP SINGER, DE (reprint author), MASSACHUSETTS GEN HOSP,GEN INTERNAL MED UNIT,BULFINCH 1,BOSTON,MA 02114, USA. NR 43 TC 215 Z9 217 U1 1 U2 4 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD FEB PY 1992 VL 41 IS 2 BP 202 EP 208 DI 10.2337/diabetes.41.2.202 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HA610 UT WOS:A1992HA61000012 PM 1733810 ER PT J AU EVERHART, JE PETTITT, DJ BENNETT, PH KNOWLER, WC AF EVERHART, JE PETTITT, DJ BENNETT, PH KNOWLER, WC TI DURATION OF OBESITY INCREASES THE INCIDENCE OF NIDDM SO DIABETES LA English DT Article ID GLUCOSE-INTOLERANCE; AMERICAN-INDIANS; PREVALENCE AB The effect of duration of obesity on incidence of non-insulin-dependent diabetes mellitus (NIDDM) was determined among Pima Indians. Duration of obesity was defined as the time since body mass index (BMI) was first known to be at least 30 kg/m2. Among 1057 participants eligible for study, there were 224 incident cases of NIDDM in 5975 person-yr of follow-up. The association of duration of obesity with incidence of diabetes adjusted for age, sex, and current BMI was highly significant (P < 0.0001). This adjusted incidence of diabetes in cases/1000 person-yr of obesity was 24.8 for people with less < 5 yr of obesity, 35.2 for people with 5-10 yr of obesity, and 59.8 for people with at least 10 yr of obesity. There was no apparent excess risk of diabetes for people who had a BMI of at least 30 kg/m2 and then lost weight. They had a slightly nonsignificantly higher rate than people who had not attained a BMI of at least 30 kg/m2 and a lower rate than people whose BMI remained 30-35 kg/m2. The relationship of duration of obesity with serum insulin concentrations among nondiabetic people was determined controlling for sex and age, BMI, and plasma glucose concentrations at the time of a glucose tolerance test. Duration of obesity was inversely associated with fasting serum insulin concentration through most of the range of fasting plasma glucose concentrations (P < 0.001) and tended to be inversely associated with 2-h postload serum insulin concentration through the entire range of postload plasma glucose concentrations (P = 0.058). C1 NIDDKD,PHOENIX EPIDEMIOL & CLIN RES BRANCH,DIABET & ARTHRITIS EPIDEMIOL SECT,PHOENIX,AZ. RP EVERHART, JE (reprint author), NIADDKD,DIV DIGEST DIS & NUTR,EPIDEMIOL & DATA SYST PROGRAM,ROOM 34 10,WESTWOOD BLDG,BETHESDA,MD 20892, USA. NR 18 TC 116 Z9 116 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD FEB PY 1992 VL 41 IS 2 BP 235 EP 240 DI 10.2337/diabetes.41.2.235 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HA610 UT WOS:A1992HA61000017 PM 1733815 ER PT J AU BROOKS, SD HARFORD, TC AF BROOKS, SD HARFORD, TC TI OCCUPATION AND ALCOHOL-RELATED CAUSES OF DEATH SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE ALCOHOL-RELATED MORTALITY; OCCUPATION; STATE OF CALIFORNIA; POPULATION-BASED STUDY ID DRINKING; WORK AB The purpose of the present investigation is to determine if various alcohol-related causes of death are associated with similar occupational groups. The California Occupational Mortality Study data set (1979-1981) provided information on primary occupation and deaths from cirrhosis, digestive cancers, injury, suicide and homicide. Age-adjusted mortality rates were calculated along with 95% confidence intervals. Findings indicate that farming/forestry/fishing personnel and handlers/equipment cleaners/helpers/labourers seem to be at risk of dying from alcohol-related causes. Due to the various methodological issues discussed, these findings need to be interpreted with caution. C1 NIAAA,DIV BIOMETRY & EPIDEMIOL,ROCKVILLE,MD 20857. RP BROOKS, SD (reprint author), CSR INC,ALCOHOL EPIDEMIOL DATA SYST,1400 EYE ST,STE 600,WASHINGTON,DC 20005, USA. NR 10 TC 4 Z9 4 U1 0 U2 2 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD FEB PY 1992 VL 29 IS 3 BP 245 EP 251 DI 10.1016/0376-8716(92)90098-W PG 7 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA HG627 UT WOS:A1992HG62700005 PM 1559430 ER PT J AU WASSERMANN, EM MCSHANE, LM HALLETT, M COHEN, LG AF WASSERMANN, EM MCSHANE, LM HALLETT, M COHEN, LG TI NONINVASIVE MAPPING OF MUSCLE REPRESENTATIONS IN HUMAN MOTOR CORTEX SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE MAGNETIC STIMULATION; MAPPING; HUMAN MOTOR CORTEX; MOTOR REPRESENTATIONS ID MAGNETIC STIMULATION; AREAS AB We used transcranial magnetic stimulation to map the cortical representations of 4 upper extremity muscles (abductor pollicis brevis, flexor carpi radialis, biceps, and deltoid) of 10 normal subjects. Three stimuli were delivered to scalp positions 1 cm apart, and the amplitude and latency of the motor evoked potentials (MEPs) were averaged for each position. Maps were described in terms of number of excitable scalp positions, amplitude of MEPs, scalp positions for evoking largest amplitude MEPs, and threshold for producing MEPs. We compared different muscles across subjects and the same muscles on the left and right sides in individual subjects. Distal muscles had larger representations with higher amplitude MEPs and lower thresholds. Biceps and deltoid on the left had larger representations and higher MEP amplitudes than on the right. Maps showed a somatotopic progression on the scalp of proximal to distal muscles along a posteromedial to anterolateral axis. C1 NINCDS,BIOMETRY & FIELD STUDIES BRANCH,BETHESDA,MD 20892. RP WASSERMANN, EM (reprint author), NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,HUMAN CORTICAL PHYSIOL UNIT,BLDG 10,BETHESDA,MD 20892, USA. NR 27 TC 359 Z9 362 U1 1 U2 17 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD FEB PY 1992 VL 85 IS 1 BP 1 EP 8 DI 10.1016/0168-5597(92)90094-R PG 8 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA HH295 UT WOS:A1992HH29500001 PM 1371738 ER PT J AU BRASILNETO, JP MCSHANE, LM FUHR, P HALLETT, M COHEN, LG AF BRASILNETO, JP MCSHANE, LM FUHR, P HALLETT, M COHEN, LG TI TOPOGRAPHIC MAPPING OF THE HUMAN MOTOR CORTEX WITH MAGNETIC STIMULATION - FACTORS AFFECTING ACCURACY AND REPRODUCIBILITY SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE MAPPING; MOTOR CORTEX; MAGNETIC STIMULATION AB We recorded motor evoked potentials (MEPs) from deltoid, biceps brachii, abductor pollicis brevis and flexor carpi radialis muscles of 5 normal volunteers during transcranial magnetic stimulation. With the subjects at rest, an 8-shaped magnetic coil was used to deliver 30 stimuli to different scalp positions 0.5 or 1.0 cm apart. The variability in amplitude and latency of MEPs was studied as a function of the scalp position stimulated, the number of stimuli at each position, and the percentage of maximal peripheral M responses (%M) elicited. The results were used to estimate the optimal number of stimuli at each position and the optimal spacing of scalp positions for topographic mapping of the human motor cortex. The amplitude and latency variability of MEPs were higher when suboptimal scalp positions were stimulated. Consequently, a larger number of stimuli were required to determine representative MEP amplitudes at suboptimal positions. In addition, there was an inverse relationship between %M recruited by transcranial magnetic stimuli in different subjects and the variability in MEP amplitude and latency. Latency variability was less pronounced than amplitude variability. Optimal sampling conditions are required to produce the best topographic maps, particularly to show subtle reorganization patterns in the human motor cortex. C1 NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,HUMAN CORTICAL PHYSIOL UNIT,BLDG 10,BETHESDA,MD 20892. NINCDS,BIOMETRY & FIELD STUDIES BRANCH,BETHESDA,MD 20892. NR 24 TC 209 Z9 212 U1 1 U2 9 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD FEB PY 1992 VL 85 IS 1 BP 9 EP 16 DI 10.1016/0168-5597(92)90095-S PG 8 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA HH295 UT WOS:A1992HH29500002 PM 1371748 ER PT J AU PANIZZA, M NILSSON, J ROTH, BJ BASSER, PJ HALLETT, M AF PANIZZA, M NILSSON, J ROTH, BJ BASSER, PJ HALLETT, M TI RELEVANCE OF STIMULUS-DURATION FOR ACTIVATION OF MOTOR AND SENSORY FIBERS - IMPLICATIONS FOR THE STUDY OF H-REFLEXES AND MAGNETIC STIMULATION SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE THRESHOLD; STIMULUS DURATION; MOTOR FIBERS; SENSORY FIBERS; MAGNETIC STIMULATION; H-REFLEXES ID ELECTRICAL-STIMULATION; NERVE; MODEL AB Electric stimuli with durations of 0.5-1.0 msec are optimal for studies of H-reflexes. It is more difficult to obtain H-reflexes with shorter duration stimuli or with magnetic stimulation. In order to understand this behavior, we studied the excitation thresholds for motor and sensory fibers in the ulnar, median and tibial nerves using both electric and magnetic stimulation. For short duration electrical stimuli (0.1 msec) the threshold for motor fibers is lower than for sensory fibers. For longer duration electric stimuli (1.0 msec) the threshold for sensory fibers is lower. For magnetic stimulation the threshold for motor fibers is much lower than for sensory fibers. Thus, stimulus duration is a critical parameter for sensory fiber excitation, and current magnetic stimulators are not optimal. C1 NINCDS,HUMAN MOTOR CONTROL SECT,BLDG 10,ROOM 5N226,BETHESDA,MD 20892. FDN CLIN LAVORO,CAMPOLI,ITALY. NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. RI Roth, Bradley/A-4920-2008; Basser, Peter/H-5477-2011 NR 17 TC 70 Z9 71 U1 1 U2 8 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD FEB PY 1992 VL 85 IS 1 BP 22 EP 29 DI 10.1016/0168-5597(92)90097-U PG 8 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA HH295 UT WOS:A1992HH29500004 PM 1371740 ER PT J AU FUHR, P COHEN, LG DANG, N FINDLEY, TW HAGHIGHI, S ORO, J HALLETT, M AF FUHR, P COHEN, LG DANG, N FINDLEY, TW HAGHIGHI, S ORO, J HALLETT, M TI PHYSIOLOGICAL ANALYSIS OF MOTOR REORGANIZATION FOLLOWING LOWER-LIMB AMPUTATION SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE MOTOR CORTEX; PLASTICITY; AMPUTATION; H-REFLEX; MAGNETIC STIMULATION ID H-REFLEX; REPRESENTATION PATTERNS; MAGNETIC STIMULATION; NERVE INJURY; CORTEX; EXCITABILITY; INHIBITION; RIGIDITY AB It is now known that amputation results in reorganization of central motor pathways, but the mechanism for the changes is unclear. One possibility is alteration of the excitability of the alpha motoneurons. We studied motor reorganization and excitability of alpha motoneurons to Ia input in 6 subjects with unilateral lower limb amputation. A Cadwell MES-10 stimulator was used to deliver transcranial magnetic stimuli through a circular coil centered on the sagittal axis 4 cm anterior to Cz and through an 8-shaped coil positioned over scalp locations 1 cm apart along the coronal axis. Surface EMG was recorded bilaterally from quadriceps femoris, the first muscle immediately proximal to the site of amputation. Excitability of the spinal alpha motoneuron pool to Ia afferents was assessed by determining the ratio of the maximal H reflex to the maximal M response (H/M ratio) elicited in the quadriceps femoris. Stimuli of equal intensity delivered to optimal scalp positions recruited a larger percentage of the alpha motoneuron pool in muscles ipsilateral to the stump than in those contralateral to the stump (P < 0.01). Mean onset latencies of motor evoked potentials were shorter in ipsilateral muscles than in contralateral muscles (P < 0.01). Muscles ipsilateral to the stump showed a trend toward activation from a larger number of scalp positions than those contralateral to the stump (P = 0.06). There was no difference in the quadriceps H/M ratios (7.2% ipsilateral vs. 10.9% contralateral). The absence of changes in the excitability of the alpha motoneuron pool in the presence of motor reorganization targeting muscles proximal to the stump suggests that reorganization occurs proximal to the alpha motoneuron level. C1 NINCDS,MED NEUROL BRANCH,HUMAN MOTOR CONTROL SECT,HUMAN CORTICAL PHYSIOL UNIT,BLDG 10,BETHESDA,MD 20892. KESSLER INST REHABIL,W ORANGE,NJ. UNIV MISSOURI,DIV NEUROSURG,COLUMBIA,MO 65201. NR 31 TC 86 Z9 87 U1 3 U2 10 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD FEB PY 1992 VL 85 IS 1 BP 53 EP 60 DI 10.1016/0168-5597(92)90102-H PG 8 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA HH295 UT WOS:A1992HH29500009 PM 1371745 ER PT J AU TSUKAMOTO, K JACKSON, IJ URABE, K MONTAGUE, PM HEARING, VJ AF TSUKAMOTO, K JACKSON, IJ URABE, K MONTAGUE, PM HEARING, VJ TI A 2ND TYROSINASE-RELATED PROTEIN, TRP-2, IS A MELANOGENIC ENZYME TERMED DOPACHROME TAUTOMERASE SO EMBO JOURNAL LA English DT Article DE DOPACHROME TAUTOMERASE; MELANOGENESIS; PIGMENTATION; TRP-2 ID ENCODING MOUSE TYROSINASE; MAMMALIAN TYROSINASE; MELANOMA-CELLS; 5,6-DIHYDROXYINDOLE-2-CARBOXYLIC ACID; CONVERSION FACTOR; BROWN LOCUS; WILD-TYPE; CDNA; BIOSYNTHESIS; CLONE AB The production of melanin pigment in mammals requires tyrosinase, an enzyme which hydroxylates the amino acid tyrosine to DOPA (3,4-dihydroxyphenylalanine), thus allowing the cascade of reactions necessary to synthesize that biopolymer. However, there are other regulatory steps that follow the action of tyrosinase and modulate the quantity and quality of the melanin produced. DOPAchrome tautomerase is one such melanogenic enzyme that isomerizes the pigmented intermediate DOPAchrome to DHICA (5,6-dihydroxyindole-2-carboxylic acid) rather than to DHI (5,6-dihydroxyindole), which would be generated spontaneously. This enzyme thus regulates a switch that controls the proportion of carboxylated subunits in the melanin biopolymer. Efforts to clone the gene for tyrosinase have resulted in the isolation of a family of tyrosinase related genes which have significant homology and encode proteins with similar predicted structural characteristics. Using specific antibodies generated against synthetic peptides encoded by unique areas of several of those proteins, we have immuno-affinity purified them and studied their melanogenic catalytic functions. We now report that TRP-2 (tyrosinase related protein-2), which maps to and is mutated at the slaty locus in mice, encodes a protein with DOPAchrome tautomerase activity. C1 WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND. RP TSUKAMOTO, K (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 37 TC 389 Z9 393 U1 3 U2 7 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD FEB PY 1992 VL 11 IS 2 BP 519 EP 526 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HE193 UT WOS:A1992HE19300017 PM 1537333 ER PT J AU JACKSON, IJ CHAMBERS, DM TSUKAMOTO, K COPELAND, NG GILBERT, DJ JENKINS, NA HEARING, V AF JACKSON, IJ CHAMBERS, DM TSUKAMOTO, K COPELAND, NG GILBERT, DJ JENKINS, NA HEARING, V TI A 2ND TYROSINASE-RELATED PROTEIN, TRP-2, MAPS TO AND IS MUTATED AT THE MOUSE SLATY LOCUS SO EMBO JOURNAL LA English DT Article DE DOPACHROME TAUTOMERASE; MELANOCYTES; MOUSE GENETICS; MUTATIONS; PIGMENTATION ID IA OCULOCUTANEOUS ALBINISM; DOPACHROME CONVERSION; MELANIN BIOSYNTHESIS; FUNCTIONAL-ANALYSIS; MOLECULAR-BASIS; POINT MUTATION; WILD-TYPE; GENE; CDNA; MELANOCYTES AB We have cloned and sequenced mouse cDNAs corresponding to a third member of a family of melanocyte-specific mRNAs, which encode tyrosinase and related proteins. This new member, tyrosinase-related protein-2 (TRP-2), has approximately 40% amino acid identity with the two other proteins in the family and has the same structural features including two copper binding sites, two cysteine-rich regions, a signal peptide and a transmembrane domain. We now show that one of the cysteine-rich regions in this protein family is an 'EGF-like' repeat found in many extracellular and cell surface proteins. The gene encoding TRP-2 maps to mouse chromosome 14, in the region of the coat colour mutation slaty. We show that the TRP-2 of slaty mice has a single amino acid difference from wild-type TRP-2; a substitution of glutamine for arginine in the first copper binding site. TRP-2 is the much sought melanogenic enzyme DOPAchrome tautomerase (DT), which catalyses the conversion of DOPAchrome to 5,6,dihydroxyindole-2-carboxylic acid. Extracts from mice homozygous for the slaty mutation have a 3-fold or more reduction in DT activity, indicating that TRP-2/DT is encoded at the slaty locus, and the missense mutation reduces but does not abolish the enzyme activity. C1 NCI,CELL BIOL LAB,BETHESDA,MD 20892. NCI,FREDERICK CANC RES & DEV CTR,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. RP JACKSON, IJ (reprint author), WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,CREWE RD,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND. FU NCI NIH HHS [N01-CO-74101] NR 50 TC 253 Z9 257 U1 2 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD FEB PY 1992 VL 11 IS 2 BP 527 EP 535 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HE193 UT WOS:A1992HE19300018 PM 1537334 ER PT J AU ARAKI, H ROPP, PA JOHNSON, AL JOHNSTON, LH MORRISON, A SUGINO, A AF ARAKI, H ROPP, PA JOHNSON, AL JOHNSTON, LH MORRISON, A SUGINO, A TI DNA POLYMERASE-II, THE PROBABLE HOMOLOG OF MAMMALIAN DNA POLYMERASE-EPSILON, REPLICATES CHROMOSOMAL DNA IN THE YEAST SACCHAROMYCES-CEREVISIAE SO EMBO JOURNAL LA English DT Article DE CELL CYCLE; CHROMOSOMAL REPLICATION; DNA POLYMERASE; SACCHAROMYCES-CEREVISIAE ID CELL NUCLEAR ANTIGEN; SIMIAN VIRUS-40; ESCHERICHIA-COLI; FUNCTIONAL DOMAINS; AUXILIARY PROTEIN; STRAND SYNTHESIS; GENE-EXPRESSION; SV40 ORIGIN; CDC2 GENE; ALPHA AB Two temperature-sensitive DNA polymerase II mutants (pol2-9 and pol2-18) of the yeast Saccharomyces cerevisiae were isolated by the plasmid shuffling method. DNA polymerase II activity partially purified from both mutants was thermolabile, while DNA polymerase I and III activities remained thermotolerant. At the restrictive temperature, the pol2 mutants were defective in chromosomal DNA replication and exhibited the dumbbell terminal morphology typical of DNA replication mutants. The POL2 transcript accumulated periodically during the cell cycle, peaking at the G1/S boundary in the same manner as the transcripts of more than 10 other DNA replication genes. These results indicate that DNA polymerase II participates in nuclear DNA replication. The similarities in structure and activities between the DNA polymerases of yeast and mammals make it likely that mammalian DNA polymerase-epsilon too is required for chromosomal DNA replication. C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. NATL INST MED RES,YEAST GENET LAB,LONDON NW7 1AA,ENGLAND. OSAKA UNIV,FAC ENGN,DEPT BIOTECHNOL,SUITA,OSAKA 565,JAPAN. OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MOLEC IMMUNOL,SUITA,OSAKA 565,JAPAN. NR 55 TC 135 Z9 137 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD FEB PY 1992 VL 11 IS 2 BP 733 EP 740 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA HE193 UT WOS:A1992HE19300041 PM 1537345 ER PT J AU OPARA, EC HUBBARD, VS BURCH, WM AKWARI, OE AF OPARA, EC HUBBARD, VS BURCH, WM AKWARI, OE TI CHARACTERIZATION OF THE INSULINOTROPIC POTENCY OF POLYUNSATURATED FATTY-ACIDS SO ENDOCRINOLOGY LA English DT Article ID PERFUSED RAT PANCREAS; ARACHIDONIC-ACID; B-CELL; CYSTIC-FIBROSIS; SECRETION; GLUCOSE; RELEASE; HYPERGLYCEMIA; ISLETS; DESENSITIZATION AB In this study we have assessed the individual abilities of the essential fatty acids, linoleic and linolenic acids, to release insulin and compared their insulinotropic potencies with those of the more established nutrient insulin secretagogues, glucose and arginine. In each experiment, a total of six islets microdissected from three mice were preperifused at the rate of 1 ml/min with Krebs-Ringer bicarbonate buffer, pH 7.4, containing 2% bovine albumin and 5.5 mM glucose (basal) with a continuous supply of 95% O2-5% CO2 at 37 C for 1 h. After collecting basal samples, the effects of 27.7 mM glucose, 20 mM arginine, 10 mM linoleic acid (18:2,omega-6), and 5 mM linolenic acid (18:3,omega-3) were tested using a sandwich protocol that entails 20-min alternating periods of stimulation with a secretagogue and a washout with basal perifusion. These nutrient concentrations were selected from initial experiments performed to characterize their dose-response effects on insulin secretion. Effluent samples were collected throughout each experiment for measurement of insulin by RIA. In one series of experiments, islets were challenged three times with 27.7 mM glucose, 10 mM linoleic acid, and 5 mM linolenic acid. In another set of experiments, islets were perifused with 20 mM arginine, 27.7 mM glucose, and 10 mM linoleic acid. All of these nutrients stimulated insulin release in a dose-dependent manner. In comparing the insulinotropic potencies of these secretagogues, we assessed insulin secretion as the integrated areas under the curve during 20 min of perifusion with a given nutrient. Thus, the mean integrated area under the curve per 20 min above basal in the presence of 27.7 mM glucose was 6,516 +/- 1,435 pg, which was not significantly different from the value of 4,772 +/- 866 pg obtained during arginine perifusion. However, the area under the curve during 20 min above basal obtained in the presence of linoleate and linolenic acid (8,712 +/- 1,949 and 10,506 +/- 1,490 pg, respectively) were significantly different (P < 0.05) from those calculated during arginine and glucose perifusions. There was no statistically significant difference between the effects of these two fatty acids at the concentrations tested. In conclusion, our data suggest that linoleic acid and linolenic acid may be, at least in this murine islet preparation, as effective in stimulating insulin release as glucose and arginine, hitherto used to assess the abilities of nutrients to stimulate insulin secretion. However, it remains to be seen whether the efficacy of these polyunsaturated fatty acids in insulin release by murine islets will be obtained in experiments performed on human islets. C1 DUKE UNIV, MED CTR, DEPT MED, DURHAM, NC 27710 USA. NIDDKD, BETHESDA, MD 20892 USA. RP OPARA, EC (reprint author), DUKE UNIV, MED CTR, DEPT SURG, BOX 3076, DURHAM, NC 27710 USA. NR 45 TC 37 Z9 37 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1992 VL 130 IS 2 BP 657 EP 662 DI 10.1210/en.130.2.657 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HH371 UT WOS:A1992HH37100014 PM 1733714 ER PT J AU VANECEK, J KLEIN, DC AF VANECEK, J KLEIN, DC TI MELATONIN INHIBITS GONADOTROPIN-RELEASING HORMONE-INDUCED ELEVATION OF INTRACELLULAR CA-2+ IN NEONATAL RAT PITUITARY-CELLS SO ENDOCRINOLOGY LA English DT Article ID CYTOSOLIC FREE CALCIUM; LUTEINIZING-HORMONE; BINDING-SITES; G-PROTEIN; RECEPTORS; ACCUMULATION; SOMATOSTATIN; ACTIVATION; RESPONSES; SECRETION AB GnRH stimulates LH release by increasing intracellular Ca2+ ([Ca2+]i). Melatonin is known to inhibit GnRH-stimulated LH release from neonatal rat pituitary cells. In the present report, the issue of whether melatonin acts through [Ca2+]i was addressed. [Ca2+]i was studied in cells in suspension, using Fluo-3 as a fluorescent indicator. In neonatal rat pituitary cells, melatonin inhibited the GnRH-induced [Ca2+]i increase in a dose-dependent manner; the GnRH-induced increase in [Ca2+]i was inhibited 40% by 100 nM melatonin. The relative potencies of several indoles as inhibitors of the GnRH stimulation of [Ca2+]i in neonatal pituitary cells (2-iodomelatonin > melatonin > 6-hydroxymelatonin) correlate with their known potencies to inhibit LH release and with their binding affinity to high affinity melatonin receptors, which indicates that these receptors probably mediate the effects of melatonin. Further support for this interpretation comes from the observation that melatonin does not inhibit the GnRH effect on [Ca2+]i in cells obtained from adolescent rat pituitary glands, which lack melatonin receptors and are insensitive to melatonin as an inhibitor of GnRH-stimulated LH release. The possible involvement of an inhibitory G-protein was also investigated by studying the effects of pertussis toxin. Pretreatment with pertussis toxin antagonized the effects of melatonin on [Ca2+]i and LH release. This indicates that melatonin may inhibit the GnRH-induced increase in [Ca2+]i through a mechanism involving a pertussis toxin-sensitive G-protein. To examine the role of extracellular Ca2+ in this effect, the effects of melatonin were examined in a low Ca2+ medium. Under these conditions, the effect of melatonin was markedly reduced, which indicates that melatonin may act by inhibiting Ca2+ influx. These observations indicate that melatonin inhibits GnRH stimulation of [Ca2+]i in neonatal rat gonadotrophs, and this probably explains the inhibitory action of melatonin on GnRH stimulation of LH release. C1 NICHHD, DEV NEUROBIOL LAB, NEUROENDOCRINOL SECT, BETHESDA, MD 20892 USA. NR 33 TC 78 Z9 79 U1 0 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1992 VL 130 IS 2 BP 701 EP 707 DI 10.1210/en.130.2.701 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HH371 UT WOS:A1992HH37100020 PM 1733718 ER PT J AU BEIL, FU FOJO, SS BREWER, HB GRETEN, H BEISIEGEL, U AF BEIL, FU FOJO, SS BREWER, HB GRETEN, H BEISIEGEL, U TI APOLIPOPROTEIN-C-II DEFICIENCY SYNDROME DUE TO APO-C-IIHAMBURG - CLINICAL AND BIOCHEMICAL FEATURES AND HPHL RESTRICTION ENZYME POLYMORPHISM SO EUROPEAN JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE APOLIPOPROTEIN-C-II DEFICIENCY; HPHI RESTRICTION ENZYME POLYMORPHISM; HYPERCHYLOMICRONEMIA; HYPERTRIGLYCERIDEMIA; PANCREATITIS ID HIGH-DENSITY LIPOPROTEINS; PLASMA-LIPOPROTEINS; LIPASE; GENE; CII; HYPERTRIGLYCERIDEMIA; MUTATION; PATIENT; HYPERLIPOPROTEINEMIA; ULTRACENTRIFUGATION AB We have characterized the clinical and biochemical features of three siblings of a kindred with severe hypertriglyceridaemia due to apolipoprotein C-II (apo C-II) deficiency caused by the mutation described as apo C-II(Hamburg). The clinical syndrome is characterized by recurrent pancreatitis in two of three affected individuals, with discrete hepatosplenomegaly in all three patients and cholelithiasis in one. Eruptive xanthomas and lipemia retinalis were absent. Plasma lipoproteins were characterized by fasting chylomicronaemia, reduced low density lipoproteins (LDL) and low high density lipoproteins (HDL). The marked hypertriglyceridaemia could be corrected promptly by infusion of normal plasma. Apolipoprotein C-II (apo C-II) levels in homozygotes were very low (0.01 mg dl-1), and mean apo C-II levels in heterozygotes were lower (2.08 +/- 0.11 mg dl-1) than in normal family members (3.38 +/- 0.75 mg dl-1). Lipoprotein lipase and hepatic triglyceride lipase activities in post-heparin plasma were normal. Zonal ultracentrifugation revealed a marked increase in triglyceride-rich lipoproteins and reduced LDL and HDL. LDL consisted of two fractions with higher hydrated density of the main fraction compared with normals with a trend to normalization on a fat-free diet. The molecular defect in the apo C-II Hamburg gene has been previously identified as a donor splice site mutation in the second intron. This leads to abnormal splicing of the apo C-II Hamburg mRNA and apo C-II deficiency in plasma. The mutation causes the loss of an HphI restriction enzyme site present in the normal apo C-II gene. Digestion of polymerase chain reaction (PCR)-amplified DNA of members of the Hamburg kindred with HphI permitted the identification of non-obligate heterozygote individuals which could not be recognized by either fasting triglyceride or apo C-II plasma levels. C1 NHLBI,MOLEC DIS BRANCH,BETHESDA,MD 20892. RP BEIL, FU (reprint author), UNIV HAMBURG,KRANKENHAUS EPPENDORF,MED KERNKLIN,MARTINISTR 52,W-2000 HAMBURG 20,GERMANY. NR 38 TC 18 Z9 18 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0014-2972 J9 EUR J CLIN INVEST JI Eur. J. Clin. Invest. PD FEB PY 1992 VL 22 IS 2 BP 88 EP 95 DI 10.1111/j.1365-2362.1992.tb01941.x PG 8 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA HE564 UT WOS:A1992HE56400003 PM 1349286 ER PT J AU LARDELLI, P STEINBERG, SM CAMPELO, C DEARANGUIZ, AF SARRIA, L GORRINO, MT CISTERNA, R AF LARDELLI, P STEINBERG, SM CAMPELO, C DEARANGUIZ, AF SARRIA, L GORRINO, MT CISTERNA, R TI EVIDENCE OF AN INVITRO ASSOCIATION BETWEEN HUMAN-IMMUNODEFICIENCY-VIRUS ANTIGEN-P24 AND EPSTEIN-BARR-VIRUS DNA SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Article ID LYMPHADENOPATHY-ASSOCIATED VIRUS; HOMOSEXUAL MEN; AIDS; TYPE-1; INFECTION; DISEASES AB To investigate the association between human immunodeficiency virus (HIV) and Epstein-Barr virus (EBV), simultaneous determinations of HIV antigen (HIV Ag)p24 and EBV DNA were performed in lymphocyte culture supernatants from 63 individuals at risk of HIV infection. In vitro data, together with HIV immune status results, were subjected to a statistical analysis. HIV infection was identified in 49 patients (78 %); of these, in vitro EBV DNA was found in 44 individuals (90 %), while in only 3 of the 14 non-infected ones (21 %). Statistical analysis demonstrated a close relationship between evidence of HIV infection and in vitro detection of EBV DNA (87.3% concordant with 95 % confidence interval: 76.5 %-94.5%). Furthermore, a strong dependence was revealed between the presence of EBV DNA and HIV Ag in culture (p < 0.00001). These results indicate the existence of in vitro viral interactions, with likely in vivo implications in the pathogenesis and evolution of HIV infection. C1 NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. RP LARDELLI, P (reprint author), HOSP CIVIL BILBAO,DEPT MICROBIOL & IMMUNOL,18 MONTEVIDEO AVE,E-48013 BILBAO,SPAIN. NR 14 TC 2 Z9 2 U1 0 U2 0 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD FEB PY 1992 VL 11 IS 2 BP 157 EP 161 DI 10.1007/BF01967068 PG 5 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA HN542 UT WOS:A1992HN54200010 PM 1327786 ER PT J AU WILDE, DB ROBERTS, K STURMHOFEL, K KIKUCHI, G COLIGAN, JE SHEVACH, EM AF WILDE, DB ROBERTS, K STURMHOFEL, K KIKUCHI, G COLIGAN, JE SHEVACH, EM TI MOUSE AUTOREACTIVE GAMMA/DELTA-T-CELLS .1. FUNCTIONAL-PROPERTIES OF AUTOREACTIVE T-CELL HYBRIDOMAS SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Article ID DENDRITIC EPIDERMAL-CELLS; GAMMA-DELTA-LYMPHOCYTES; ANTIGEN RECEPTOR; INTRAEPITHELIAL LYMPHOCYTES; MYCOBACTERIUM-TUBERCULOSIS; LIMITED DIVERSITY; EXPRESSION; SPECIFICITY; RECOGNITION; THYMOCYTES AB A minor population of dendritic epidermal T cells (DETC) express the V(gamma)1.1C(gamma)4V(delta)6T cell receptor and T cell clones and hybridomas derived from this subset constitutively secrete cytokines in culture secondary to recognition of an autoantigen. Activation of these autoreactive cells requires the use of the vitronection receptor (VNR) as an accessory molecule which interacts with the Arg-Gly-Asp-Ser (RGDS) sequence of extracellular matrix (ECM) proteins. We have compared the functional properties of C(gamma)4+ hybridomas derived from newborn thymocytes and from adult spleen with the DETC hybridomas/lines in terms of their ability to secrete cytokines spontaneously and for the use of the VNR as an accessory molecule. Almost all of the C(gamma)4+ thymocyte hybridomas secreted cytokines spontaneously and in the majority of lines the most prominent cytokine secreted was granulocyte-monocyte colony-stimulating factor. In contrast, none of the four splenic C(gamma)4+ hybridomas secreted cytokines spontaneously although all were capable of cytokine production following activation via the T cell receptor. Although the thymocyte hybridomas did not grow as adherent cell lines in culture, constitutive cytokine production required engagement of the VNR by its ligand in ECM proteins. In all cases, cytokine production could be inhibited by an anti-VNR monoclonal antibody as well as by soluble RGDS. The strong correlation of functional and molecular properties (see accompanying report) between thymocyte C(gamma)4+ hybridomas and C(gamma)4+ DETC suggests that the C(gamma)4+ DETC may be of thymic origin and that cells with potential for autoreactivity residing in the thymus at birth may populate other peripheral locations in the mouse. The data also support the concept that the VNR, and possibly other integrins, play a role as accessory elements for autoreactive cells and may be essential for the regulation of such activity. C1 NIAID,IMMUNOL LAB,BLG 10,ROOM 11N315,BETHESDA,MD 20892. NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. NR 44 TC 29 Z9 29 U1 1 U2 2 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0014-2980 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD FEB PY 1992 VL 22 IS 2 BP 483 EP 489 DI 10.1002/eji.1830220229 PG 7 WC Immunology SC Immunology GA HF952 UT WOS:A1992HF95200028 PM 1371469 ER PT J AU EZQUERRA, A WILDE, DB MCCONNELL, TJ STURMHOFEL, K VALAS, RB SHEVACH, EM COLIGAN, JE AF EZQUERRA, A WILDE, DB MCCONNELL, TJ STURMHOFEL, K VALAS, RB SHEVACH, EM COLIGAN, JE TI MOUSE AUTOREACTIVE GAMMA/DELTA-T-CELLS .2. MOLECULAR CHARACTERIZATION OF THE T-CELL RECEPTOR SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Article ID DENDRITIC EPIDERMAL-CELLS; GAMMA-DELTA; ANTIGEN RECEPTOR; INTRAEPITHELIAL LYMPHOCYTES; VARIABLE REGION; BETA-CHAIN; EXPRESSION; GENES; SPECIFICITY; RECOGNITION AB A panel of dendritic epidermal T cell (DETC) lines, and hybridomas derived from them, has been shown to spontaneously secrete lymphokines in the absence of added stimuli, which suggests that these cells are autoreactive. These cell lines are characterized by the expression of a V(gamma)1.1C(gamma)4/V(delta)6 type T cell receptor (TcR), but several of the DETC lines also express a second TcR. Sequence analyses of these gamma/delta TcR revealed that the gamma-chains were identical and that the delta-chains, while not identical, were quite restricted in diversity, indicating that these receptors may recognize a common or closely related group of antigens. Analysis of hybridomas derived from newborn thymocytes identified six hybridomas that spontaneously secrete lymphokines. Five hybrids expressed a V(gamma)1.1C(gamma)4/V(delta)6 receptor and one hybrid a V(gamma)1.1C(gamma)4/V(delta)4 receptor that had a close structural relationship to the DETC-gamma/delta TcR associated with spontaneous lymphokine secretion. Gamma/delta TcR of the C(gamma)4 type expressed by splenic hybridomas that did not spontaneously secrete lymphokines revealed no such relationship. Curiously, like the DETC, several of the thymocyte hybridomas that spontaneously secreted lymphokines expressed a second TcR, V(gamma)2C(gamma)1 or V(gamma)3C(gamma)1, apparently in association with the same delta-chain that paired with the C(gamma)4 chain. The presence of spontaneous lymphokine-secreting gamma/delta T cells with such highly homologous TcR in both the thymus and skin suggests a thymic origin for the autoreactive DETC and that these cells recognize a common or closely related group of self-antigens. C1 NIAID,BIOL RESOURCES BRANCH,BLDG 4,ROOM 413,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Ezquerra, Angel/I-3440-2012; OI Ezquerra, Angel/0000-0002-1824-5087 NR 43 TC 25 Z9 25 U1 0 U2 1 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0014-2980 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD FEB PY 1992 VL 22 IS 2 BP 491 EP 498 DI 10.1002/eji.1830220230 PG 8 WC Immunology SC Immunology GA HF952 UT WOS:A1992HF95200029 PM 1311262 ER PT J AU TANG, AM UDEY, MC AF TANG, AM UDEY, MC TI DIFFERENTIAL SENSITIVITY OF FRESHLY ISOLATED AND CULTURED MURINE LANGERHANS CELLS TO ULTRAVIOLET-B RADIATION AND CHEMICAL FIXATION SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Article ID ANTIGEN-PRESENTING CAPACITY; DENDRITIC CELLS; EPIDERMAL-CELLS; IRRADIATION; INVITRO; ICAM-1; LIGHT AB Previous studies have demonstrated that low doses of ultraviolet B (UVB) radiation (100 J/m2) abrogate the accessory function of freshly isolated murine epidermal Langerhans cells (fLC) and cause a parallel decrease in the ability of LC to express increased amounts of ICAM-1 (CD54) in vitro. We have subsequently observed that the accessory cell function of cultured LC (cLC), as assessed by their ability to support anti-CD3 monoclonal antibody (mAb)-induced T cell mitogenesis, was not inhibited by levels of UVB exposure (100 J/m2) that completely inhibited the function of fLC, although exposure of cLC to UVB radiation (100 J/m2) resulted in a decrease in the level of ICAM-1 expression on most cLC and a concomitant decrease in cLC survival during a subsequent 24-h incubation. Time course studies revealed that T cells stimulated with anti-CD3 mAb in the presence of cLC became committed to proliferate 4-8 h after culture initiation, while 24-30 h of co-culture was required for irreversible T cell activation when fLC were utilized as accessory cells. In addition, paraformaldehyde (PFA)-fixed (non-viable) cLC supported anti-CD3 mAb-induced T cell proliferation, whereas PFA-fixed fLC were ineffective. We propose that cLC are functionally resistant to low doses of UVB radiation and chemical fixation because cLC express sufficient levels of the adhesion or co-stimulatory molecules [including ICAM-1 and Mac-1 (CD11b/CD18)] required to induce T cell activation. Conversely, fLC are sensitive to the effects of UVB radiation and chemical fixation because these physicochemical agents prevent acquisition of critically important surface molecules in culture. C1 NCI,DERMATOL BRANCH,BLDG 10,ROOM 12N254,BETHESDA,MD 20892. NR 26 TC 22 Z9 22 U1 0 U2 0 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0014-2980 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD FEB PY 1992 VL 22 IS 2 BP 581 EP 586 DI 10.1002/eji.1830220242 PG 6 WC Immunology SC Immunology GA HF952 UT WOS:A1992HF95200041 PM 1347017 ER PT J AU LAGERGARD, T THIRINGER, K WASSEN, L SCHNEERSON, R TROLLFORS, B AF LAGERGARD, T THIRINGER, K WASSEN, L SCHNEERSON, R TROLLFORS, B TI ISOTYPE COMPOSITION OF ANTIBODIES TO STREPTOCOCCUS GROUP-B TYPE-III POLYSACCHARIDE AND TO TETANUS TOXOID IN MATERNAL, CORD BLOOD SERA AND IN BREAST-MILK SO EUROPEAN JOURNAL OF PEDIATRICS LA English DT Article DE GROUP-B STREPTOCOCCUS; TETANUS; ANTIBODIES; CLASS; SUBCLASS ID VACCINE-INDUCED ANTIBODY; HUMAN IGG SUBCLASSES; CAPSULAR POLYSACCHARIDE; INFECTION; ASSAY AB Neonates are protected against group B streptococcal (GBS) infections and tetanus by transplacentally transferred serum antibodies. Antibodies of the immunoglobulin (Ig) G, IgM and IgA classes and IgG subclasses to the capsular polysaccharide (CPS) of type III group B streptococci (GBS III) and to tetanus toxoid (TT) were measured in sera from healthy women of fertile age and in paired maternal and cord blood sera from term and preterm pregnancies. GBS III CPS antibodies of the IgG class were found in sera from 97 out of 100 women of fertile age. but only 15 of them had antibodies above the proposed protective level (greater-than-or-equal-to 2-mu-g/ml). TT IgG antibodies above the protective level (0.01 units/ml) were found in all sera. The IgG antibodies against GBS III CPS were mainly composed of the IgG2 subclass and to a lesser extent of IgG1. Almost all women had IgG1 antibodies against TT and 40% had IgG4 antibodies. Total IgG and IgG1 antibodies against GBS III CPS were higher in cord blood sera from 37 term neonates than in sera from their mothers whereas IgG2 antibody levels were similar. Total IgG and IgG1 antibodies against TT were also higher in the 20 term neonates tested than in their mothers. In contrast, total IgG and IgG1 to both GBS III CPS and TT and IgG2 to GBS III CPS were lower in cord blood sera from preterm neonates than in sera from their mothers. IgA antibodies to GBS III CPS were detected in 63% of breast milk samples while IgA antibodies against TT were detected in only 4%. In conclusion the study shows important differences in IgG subclass composition of antibodies against a polysaccharide and a protein antigen and in placental transfer of IgG antibodies in term and preterm babies. C1 GOTHENBURG UNIV,DEPT PAEDIAT,S-41346 GOTHENBURG,SWEDEN. GOTHENBURG UNIV,DEPT OBSTET & GYNAECOL,S-41346 GOTHENBURG,SWEDEN. NICHHD,BETHESDA,MD 20892. RP LAGERGARD, T (reprint author), GOTHENBURG UNIV,DEPT MED MICROBIOL,GULDHEDSGATAN 10,S-41346 GOTHENBURG,SWEDEN. NR 26 TC 16 Z9 16 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-6199 J9 EUR J PEDIATR JI Eur. J. Pediatr. PD FEB PY 1992 VL 151 IS 2 BP 98 EP 102 DI 10.1007/BF01958951 PG 5 WC Pediatrics SC Pediatrics GA HB023 UT WOS:A1992HB02300006 PM 1537371 ER PT J AU BRITTEN, KH NEWSOME, WT SAUNDERS, RC AF BRITTEN, KH NEWSOME, WT SAUNDERS, RC TI EFFECTS OF INFEROTEMPORAL CORTEX LESIONS ON FORM-FROM-MOTION DISCRIMINATION IN MONKEYS SO EXPERIMENTAL BRAIN RESEARCH LA English DT Article DE EXTRASTRIATE VISUAL CORTEX; INFEROTEMPORAL CORTEX; MOTION PROCESSING; CORTICAL PATHWAYS; LESION EFFECTS; STRUCTURE-FROM-MOTION ID VISUAL AREA MT; FOCAL BRAIN WOUNDS; MACAQUE MONKEY; EXTRASTRIATE CORTEX; RHESUS-MONKEY; CORTICAL CONNECTIONS; PARIETAL LESIONS; MACACA-MULATTA; NEURONS; PERCEPTION AB The inferotemporal cortex of primates plays a prominent role in the learning and retention of visual form discriminations. In this experiment we investigated the role of inferotemporal (IT) cortex in the discrimination of two-dimensional forms defined by motion cues. Six monkeys were trained to a criterion level of performance on two form-from-motion problems. Three of these animals received complete bilateral lesions of IT cortex, while the other three served as unoperated controls. All animals were then retrained to criterion to evaluate the effects of IT lesions on the retention of form-from-motion learning. Compared with the control group, the lesion group was significantly impaired on both problems. Following retention testing, we trained both groups of monkeys on two new form-from-motion problems to investigate the effects of IT lesions on acquisition rates for new learning. The lesion group performed well on the new problems; the learning rates of the operated and control groups were not significantly different. When forms were defined by luminance cues, monkeys with IT lesions, like those in previous studies, were impaired both for retention and for acquisition. These findings indicate that the anterograde effects of IT lesions on learning new form discriminations are less severe for forms defined by motion cues than for forms defined by luminance cues. However, the retrograde effects of IT lesions on retention are severe for forms defined by either cue. C1 ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RP BRITTEN, KH (reprint author), STANFORD UNIV,MED CTR,SCH MED,DEPT NEUROBIOL,STANFORD,CA 94305, USA. FU NEI NIH HHS [EY 5603] NR 57 TC 51 Z9 51 U1 2 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-4819 J9 EXP BRAIN RES JI Exp. Brain Res. PD FEB PY 1992 VL 88 IS 2 BP 292 EP 302 DI 10.1007/BF02259104 PG 11 WC Neurosciences SC Neurosciences & Neurology GA HF511 UT WOS:A1992HF51100006 PM 1577103 ER PT J AU KIBBEY, MC ROYCE, LS DYM, M BAUM, BJ KLEINMAN, HK AF KIBBEY, MC ROYCE, LS DYM, M BAUM, BJ KLEINMAN, HK TI GLANDULAR-LIKE MORPHOGENESIS OF THE HUMAN SUBMANDIBULAR TUMOR-CELL LINE-A253 ON BASEMENT-MEMBRANE COMPONENTS SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID EMBRYONIC SALIVARY EPITHELIA; AMINO-ACID-SEQUENCE; LAMININ-A CHAIN; EXTRACELLULAR-MATRIX; MULTIPLE RECEPTORS; CARCINOMA-CELLS; PRIMARY CULTURE; BASAL LAMINA; COLLAGEN; DIFFERENTIATION C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. GEORGETOWN UNIV,MED CTR,DEPT ANAT & CELL BIOL,WASHINGTON,DC 20007. RP KIBBEY, MC (reprint author), NIDR,DEV BIOL LAB,BLDG 30,ROOM 402,BETHESDA,MD 20892, USA. NR 41 TC 32 Z9 32 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD FEB PY 1992 VL 198 IS 2 BP 343 EP 351 PG 9 WC Oncology; Cell Biology SC Oncology; Cell Biology GA GZ599 UT WOS:A1992GZ59900020 PM 1530839 ER PT J AU PURSELL, CD MOSKOWITZ, J MAHONEY, JD GOLDSTEIN, JW AF PURSELL, CD MOSKOWITZ, J MAHONEY, JD GOLDSTEIN, JW TI CONSENSUS CONFERENCE ON RESEARCH FUNDING SO FASEB JOURNAL LA English DT Discussion C1 NIH,BETHESDA,MD 20892. ALCOHOL DRUG ABUSE & MENTAL HLTH ADM,ROCKVILLE,MD. RP PURSELL, CD (reprint author), US HOUSE REPRESENTAT,COMM APPROPRIAT,WASHINGTON,DC 20515, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 1 PY 1992 VL 6 IS 3 BP 813 EP 820 PG 8 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HE390 UT WOS:A1992HE39000002 ER PT J AU NOSSAL, NG AF NOSSAL, NG TI PROTEIN-PROTEIN INTERACTIONS AT A DNA-REPLICATION FORK - BACTERIOPHAGE-T4 AS A MODEL SO FASEB JOURNAL LA English DT Article DE DNA REPLICATION; BACTERIOPHAGE-T4; T4 DNA POLYMERASE; POLYMERASE ACCESSORY PROTEINS; PRIMASE; HELICASE; RNASE-H ID POLYMERASE ACCESSORY PROTEINS; RNA PRIMER SYNTHESIS; T4 GENE-45 PROTEIN; ESCHERICHIA-COLI; STRAND DISPLACEMENT; LEADING-STRAND; ATP HYDROLYSIS; III HOLOENZYME; 45 PROTEINS; DUPLEX DNA AB The DNA replication system of bacteriophage T4 serves as a relatively simple model for the types of reactions and protein-protein interactions needed to carry out and coordinate the synthesis of the leading and lagging strands of a DNA replication fork. At least 10 phage-encoded proteins are required for this synthesis: T4 DNA polymerase, the genes 44/62 and 45 polymerase accessory proteins, gene 32 single-stranded DNA binding protein, the genes 61, 41, and 59 primase-helicase, RNase H, and DNA ligase. Assembly of the polymerase and the accessory proteins on the primed template is a stepwise process that requires ATP hydrolysis and is strongly stimulated by 32 protein. The 41 protein helicase is essential to unwind the duplex ahead of polymerase on the leading strand, and to interact with the 61 protein to synthesize the RNA primers that initiate each discontinuous fragment on the lagging strand. An interaction between the 44/62 and 45 polymerase accessory proteins and the primase-helicase is required for primer synthesis on 32 protein-covered DNA. Thus it is possible that the signal for the initiation of a new fragment by the primase-helicase is the release of the polymerase accessory proteins from the completed adjacent fragment. RP NOSSAL, NG (reprint author), NIDDKD,BIOCHEM PHARMACOL LAB,NUCLEIC ACID BIOCHEM SECT,BETHESDA,MD 20892, USA. NR 57 TC 87 Z9 87 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 1 PY 1992 VL 6 IS 3 BP 871 EP 878 PG 8 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HE390 UT WOS:A1992HE39000010 PM 1310946 ER PT J AU MAKI, A BEREZESKY, IK FARGNOLI, J HOLBROOK, NJ TRUMP, BF AF MAKI, A BEREZESKY, IK FARGNOLI, J HOLBROOK, NJ TRUMP, BF TI ROLE OF [CA2+]I IN INDUCTION OF C-FOS, C-JUN, AND C-MYC MESSENGER-RNA IN RAT PTE AFTER OXIDATIVE STRESS SO FASEB JOURNAL LA English DT Note DE GENE EXPRESSION; FREE RADICAL; PROTOONCOGENE; CALCIUM; PROXIMAL TUBULAR EPITHELIUM ID OXYGEN FREE-RADICALS; SEQUENTIAL PROTOONCOGENE EXPRESSION; BRONCHIAL EPITHELIAL-CELLS; PROTEIN KINASE-C; HYDROGEN-PEROXIDE; XANTHINE-OXIDASE; CYTO-TOXICITY; DNA-SYNTHESIS; 3T3 CELLS; ACTIVATION AB Oxidative stress plays an important role in various types of cell injury and tumor promotion. Cells respond to oxidative stress in many ways including changes in membrane organization, ion movements, and altered gene expression, all of which contribute to the subsequent fate of affected cells. In this study, we investigated the expression of the proto-oncogenes c-fos, c-myc, and c-jun, which play a key role in proliferation and differentiation, using primary cultures of rat proximal tubular epithelium exposed to oxidative stress generated by the xanthine/xanthine oxidase system. This system generates superoxide and H2O2 in the extracellular space stimulating the release of active oxygen species from inflammatory cells. c-fos mRNA was expressed within 15 min, peaked at 30 min, and returned to constitutive levels by 3 h. c-jun mRNA began to rise after 30 min, peaked at 120 min, and remained above the constitutive levels up to 180 min. c-myc mRNA expression was less affected by the treatment, with levels increasing gradually over the 180 min period. The expression of c-fos was inhibited by superoxide dismutase but not by catalase and was super-induced by cycloheximide. H2O2 alone did not induce any c-fos mRNA in this system. Chelation of extracellular ionized calcium by EGTA or of intracellular ionized calcium by Quin 2/AM resulted in a marked decrease of c-fos expression. Two protein kinase C inhibitors, H-7 and staurosporine, partly diminished the expression of c-fos, whereas a third, 2-aminopurine, which has a broader spectrum of inhibiting protein kinases, almost completely abolished it. A poly ADP-ribosylation inhibitor, 3-aminobenzamide, had no effect on c-fos expression in this system. Our results show that oxidative stress provokes sequential expression of c-fos, c-jun, and c-myc, mRNA in this order. This c-fos expression appears to be largely controlled by calcium ion movement, which could include protein kinase C activation. Another protein kinase or kinases also appear to play an important role. C1 UNIV MARYLAND,SCH MED,DEPT PATHOL,10 S PINE ST,BALTIMORE,MD 21201. MARYLAND INST EMERGENCY MED SERV SYST,BALTIMORE,MD 21201. NIA,MOLEC GENET LAB,BALTIMORE,MD 21224. FU NIDDK NIH HHS [DK15440] NR 51 TC 150 Z9 150 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 1 PY 1992 VL 6 IS 3 BP 919 EP 924 PG 6 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA HE390 UT WOS:A1992HE39000017 PM 1740241 ER PT J AU NELSON, LM FLEISHER, TA AF NELSON, LM FLEISHER, TA TI PERIPHERAL T-LYMPHOCYTE ACTIVATION AND ESTROGEN STATUS - REPLY SO FERTILITY AND STERILITY LA English DT Letter C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,IMMUNOL SERV,BETHESDA,MD 20892. RP NELSON, LM (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,GYNECOL RES SECT,BETHESDA,MD 20892, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC REPRODUCTIVE MEDICINE PI BIRMINGHAM PA 1209 MONTGOMERY HIGHWAY, BIRMINGHAM, AL 35216-2809 SN 0015-0282 J9 FERTIL STERIL JI Fertil. Steril. PD FEB PY 1992 VL 57 IS 2 BP 464 EP 465 PG 2 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA HB430 UT WOS:A1992HB43000043 ER PT J AU BOND, JA WALLACE, LA OSTERMANGOLKAR, S LUCIER, GW BUCKPITT, A HENDERSEN, RF AF BOND, JA WALLACE, LA OSTERMANGOLKAR, S LUCIER, GW BUCKPITT, A HENDERSEN, RF TI ASSESSMENT OF EXPOSURE TO PULMONARY TOXICANTS - USE OF BIOLOGICAL MARKERS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Editorial Material ID VOLATILE ORGANIC-COMPOUNDS; ETHYLENE-OXIDE; DNA ADDUCTS; THIOETHER EXCRETION; HEMOGLOBIN ADDUCTS; ALKYLATING-AGENTS; COVALENT BINDING; GENETIC RISKS; ETHENE OXIDE; LUNG-CANCER C1 US EPA, EPIC, VINT HILL FARM STN, WARRENTON, VA 22186 USA. UNIV STOCKHOLM, CHEM IND INST TOXICOL, S-10691 STOCKHOLM, SWEDEN. UNIV STOCKHOLM, DEPT RADIOBIOL, S-10691 STOCKHOLM, SWEDEN. NIEHS, DIV BIOMETRY & RISK ASSESSMENT, RES TRIANGLE PK, NC 27709 USA. UNIV CALIF DAVIS, SCH VET MED, DEPT PHARMACOL & TOXICOL, DAVIS, CA 95616 USA. LOVELACE INHALAT TOXICOL RES INST, ALBUQUERQUE, NM 87185 USA. RP BOND, JA (reprint author), CHEM IND INST TOXICOL, 6 DAVIS DR, POB 12137, RES TRIANGLE PK, NC 27709 USA. RI Wallace, Lance/K-7264-2013; OI Wallace, Lance/0000-0002-6635-2303 NR 73 TC 10 Z9 11 U1 1 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 161 EP 174 DI 10.1016/0272-0590(92)90042-G PG 14 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000001 PM 1601216 ER PT J AU GUTIERREZESPELETA, GA HUGHES, LA PIEGORSCH, WW SHELBY, MD GENEROSO, WM AF GUTIERREZESPELETA, GA HUGHES, LA PIEGORSCH, WW SHELBY, MD GENEROSO, WM TI ACRYLAMIDE - DERMAL EXPOSURE PRODUCES GENETIC-DAMAGE IN MALE-MOUSE GERM-CELLS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID MALE-MICE; JACKKNIFE C1 OAK RIDGE NATL LAB,DIV BIOL,POB 2009,OAK RIDGE,TN 37738. NIEHS,RES TRIANGLE PK,NC 27709. UNIV COSTA RICA,ESCUELA BIOL,SAN JOSE,COSTA RICA. OI Piegorsch, Walter/0000-0003-2725-5604 FU NIEHS NIH HHS [Y01-ES-20085] NR 14 TC 25 Z9 25 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 189 EP 192 DI 10.1016/0272-0590(92)90045-J PG 4 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000004 PM 1601219 ER PT J AU LUSTER, MI PORTIER, C PAIT, DG WHITE, KL GENNINGS, C MUNSON, AE ROSENTHAL, GJ AF LUSTER, MI PORTIER, C PAIT, DG WHITE, KL GENNINGS, C MUNSON, AE ROSENTHAL, GJ TI RISK ASSESSMENT IN IMMUNOTOXICOLOGY .1. SENSITIVITY AND PREDICTABILITY OF IMMUNE TESTS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID CHEMICAL CARCINOGENICITY; TOXICOLOGY; TOXICITY; CELLS; HYPERSENSITIVITY; LYMPHOCYTES; PREDICTION; WORKSHOP; INVITRO; MICE C1 NIEHS,STAT & BIOMATH BRANCH,RES TRIANGLE PK,NC 27709. VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOSTAT,RICHMOND,VA 23298. VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PHARMACOL & TOXICOL,RICHMOND,VA 23298. RP LUSTER, MI (reprint author), NIEHS,SYST TOXIC BRANCH,RES TRIANGLE PK,NC 27709, USA. RI Portier, Christopher/A-3160-2010 OI Portier, Christopher/0000-0002-0954-0279 NR 36 TC 381 Z9 392 U1 0 U2 12 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 200 EP 210 DI 10.1016/0272-0590(92)90047-L PG 11 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000006 PM 1534777 ER PT J AU REEL, JR LAWTON, AD LAMB, JC AF REEL, JR LAWTON, AD LAMB, JC TI REPRODUCTIVE TOXICITY EVALUATION OF ACETAMINOPHEN IN SWISS CD-1 MICE USING A CONTINUOUS BREEDING PROTOCOL SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID INDUCED HEPATIC NECROSIS; METABOLIC-ACTIVATION; DEPLETION; OVERDOSE; BINDING; INVITRO; FETAL; RAT C1 RES TRIANGLE INST,CHEM & LIFE SCI GRP,POB 12194,RES TRIANGLE PK,NC 27709. NIEHS,NATL TOXICOL PROGRAM,DEV & REPROD TOXICOL GRP,RES TRIANGLE PK,NC 27709. FU NIEHS NIH HHS [N01-ES-2-5014, N01-ES-9-6515] NR 35 TC 17 Z9 17 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 233 EP 239 DI 10.1016/0272-0590(92)90051-I PG 7 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000010 PM 1601223 ER PT J AU MAST, TJ WEIGEL, RJ WESTERBERG, RB SCHWETZ, BA MORRISSEY, RE AF MAST, TJ WEIGEL, RJ WESTERBERG, RB SCHWETZ, BA MORRISSEY, RE TI EVALUATION OF THE POTENTIAL FOR DEVELOPMENTAL TOXICITY IN RATS AND MICE FOLLOWING INHALATION EXPOSURE TO TETRAHYDROFURAN SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID ORGANIC-SOLVENTS C1 NIEHS, DIV TOXICOL RES & TESTING, DEV & REPROD TOXICOL GRP, RES TRIANGLE PK, NC 27709 USA. RP MAST, TJ (reprint author), PACIFIC NW LAB, RICHLAND, WA 99352 USA. FU NIEHS NIH HHS [Y01-ES-70153] NR 27 TC 3 Z9 3 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 255 EP 265 DI 10.1016/0272-0590(92)90054-L PG 11 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000013 PM 1601226 ER PT J AU HEINDEL, JJ PRICE, CJ FIELD, EA MARR, MC MYERS, CB MORRISSEY, RE SCHWETZ, BA AF HEINDEL, JJ PRICE, CJ FIELD, EA MARR, MC MYERS, CB MORRISSEY, RE SCHWETZ, BA TI DEVELOPMENTAL TOXICITY OF BORIC-ACID IN MICE AND RATS SO FUNDAMENTAL AND APPLIED TOXICOLOGY LA English DT Article ID ZERO DOSE CONTROL; BORON; TERATOGENICITY; RIBOFLAVIN; CADMIUM C1 RES TRIANGLE INST,CTR LIFE SCI & TOXICOL,RES TRIANGLE PK,NC 27709. RP HEINDEL, JJ (reprint author), NIEHS,NATL TOXICOL PROGRAM,DEV & REPROD TOXICOL GRP,POB 12233,RES TRIANGLE PK,NC 27709, USA. FU NIEHS NIH HHS [N01-ES-95255] NR 39 TC 68 Z9 71 U1 0 U2 7 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0272-0590 J9 FUND APPL TOXICOL JI Fundam. Appl. Toxicol. PD FEB PY 1992 VL 18 IS 2 BP 266 EP 277 DI 10.1016/0272-0590(92)90055-M PG 12 WC Toxicology SC Toxicology GA HD260 UT WOS:A1992HD26000014 PM 1601227 ER PT J AU BERGASA, NV TALBOT, TL ALLING, DW SCHMITT, JM WALKER, EC BAKER, BL KORENMAN, JC PARK, Y HOOFNAGLE, JH JONES, EA AF BERGASA, NV TALBOT, TL ALLING, DW SCHMITT, JM WALKER, EC BAKER, BL KORENMAN, JC PARK, Y HOOFNAGLE, JH JONES, EA TI A CONTROLLED TRIAL OF NALOXONE INFUSIONS FOR THE PRURITUS OF CHRONIC CHOLESTASIS SO GASTROENTEROLOGY LA English DT Article ID PRIMARY BILIARY-CIRRHOSIS; PLASMA METHIONINE ENKEPHALIN; OPIOID-PEPTIDES; LIVER-DISEASE; CLONIDINE; RIFAMPIN; ITCH C1 NIAID,DIV INTRAMURAL RES,BETHESDA,MD 20892. NIDDKD,DIGEST DIS BRANCH,LIVER DIS SECT,BETHESDA,MD. NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUTMENTAT PROGRAM,APPL CLIN ENGN SECT,BETHESDA,MD. RP BERGASA, NV (reprint author), NIH,LIVER UNIT,BLDG 10,ROOM 4D52,BETHESDA,MD 20892, USA. NR 30 TC 194 Z9 196 U1 1 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD FEB PY 1992 VL 102 IS 2 BP 544 EP 549 PG 6 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA HA639 UT WOS:A1992HA63900022 PM 1732125 ER PT J AU SINGH, J KLAR, AJS AF SINGH, J KLAR, AJS TI ACTIVE GENES IN BUDDING YEAST DISPLAY ENHANCED INVIVO ACCESSIBILITY TO FOREIGN DNA METHYLASES - A NOVEL INVIVO PROBE FOR CHROMATIN STRUCTURE OF YEAST SO GENES & DEVELOPMENT LA English DT Article DE INVIVO DNA METHYLATION; GENE EXPRESSION; BUDDING YEAST; FOREIGN METHYLASES ID MATING-TYPE LOCUS; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; CELL TYPE; SEQUENCES; EXPRESSION; SITES; PROMOTER; IDENTIFICATION; TRANSPOSITION AB Unlike higher eukaryotes, where an inverse correlation has been generally observed between gene expression and methylation of CpG sites, the budding yeast Saccharomyces cerevisiae lacks DNA methylation. Gene regulatory mechanisms can function independently of DNA methylation in yeast, and yeast strains expressing foreign DNA methylases that modify adenine and CpG residues have been found to be viable. We have used such strains to determine whether the transcriptional status of genes can influence the level of their DNA methylation in vivo. Several genes were tested, for example, GAL1, -7, and -10, PHO5, HMRa and HML-alpha, and STE2 and STE3. Surprisingly, we found that all the genes displayed severalfold more methylation in the expressed state as compared to the repressed state. This procedure serves as a novel in vivo probe for the chromatin structure of yeast and potentially for higher eukaryotes. RP SINGH, J (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,EUKARYOT GENE EXPRESS,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 43 TC 159 Z9 162 U1 0 U2 2 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0890-9369 J9 GENE DEV JI Genes Dev. PD FEB PY 1992 VL 6 IS 2 BP 186 EP 196 DI 10.1101/gad.6.2.186 PG 11 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA HG934 UT WOS:A1992HG93400004 PM 1737615 ER PT J AU WHITE, MB CARVALHO, M DERSE, D OBRIEN, SJ DEAN, M AF WHITE, MB CARVALHO, M DERSE, D OBRIEN, SJ DEAN, M TI DETECTING SINGLE BASE SUBSTITUTIONS AS HETERODUPLEX POLYMORPHISMS SO GENOMICS LA English DT Article ID POLYMERASE CHAIN-REACTION; INFECTIOUS-ANEMIA VIRUS; POINT MUTATIONS; GENE; IDENTIFICATION; MISMATCHES; SEQUENCE; CLEAVAGE; EXON-9 C1 PROGRAM RESOURCES INC DYNCORP,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702. RP WHITE, MB (reprint author), NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702, USA. RI Dean, Michael/G-8172-2012 OI Dean, Michael/0000-0003-2234-0631 FU NCI NIH HHS [N01-CO-74102] NR 24 TC 334 Z9 339 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1992 VL 12 IS 2 BP 301 EP 306 DI 10.1016/0888-7543(92)90377-5 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA GY879 UT WOS:A1992GY87900014 PM 1740339 ER PT J AU MOORE, KJ DAMOREBRUNO, MA KORFHAGEN, TR GLASSER, SW WHITSETT, JA JENKINS, NA COPELAND, NG AF MOORE, KJ DAMOREBRUNO, MA KORFHAGEN, TR GLASSER, SW WHITSETT, JA JENKINS, NA COPELAND, NG TI CHROMOSOMAL LOCALIZATION OF 3 PULMONARY SURFACTANT PROTEIN GENES IN THE MOUSE SO GENOMICS LA English DT Article ID GLUTAMATE-DEHYDROGENASE GLUD; AMINO-ACID SEQUENCE; SP-C; INTERSPECIFIC BACKCROSS; LINKAGE MAP; LUNG SURFACTANT; REGION GENES; SHORT ARM; II CELLS; SP-B C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,POB B,BLDG 539,FREDERICK,MD 21702. CHILDRENS HOSP,MED CTR,COLL MED,DIV PULM BIOL,CINCINNATI,OH 45267. FU NCI NIH HHS [N01-CO-74101]; NHLBI NIH HHS [HL28623, HL3859] NR 67 TC 45 Z9 45 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1992 VL 12 IS 2 BP 388 EP 393 DI 10.1016/0888-7543(92)90389-A PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA GY879 UT WOS:A1992GY87900026 PM 1346779 ER PT J AU SPENCE, SE GILBERT, DJ HARRIS, BS DAVISSON, MT COPELAND, NG JENKINS, NA AF SPENCE, SE GILBERT, DJ HARRIS, BS DAVISSON, MT COPELAND, NG JENKINS, NA TI GENETIC LOCALIZATION OF HAO-1, BLIND-STERILE (BS), AND EMV-13 ON MOUSE CHROMOSOME-2 SO GENOMICS LA English DT Note ID STRAIN; MUTANT; LOCUS C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,POB B,BLDG 539,FREDERICK,MD 21702. JACKSON LAB,BAR HARBOR,ME 04609. FU NCI NIH HHS [N01-CO-74101]; NCRR NIH HHS [P40 RR01183] NR 10 TC 13 Z9 16 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1992 VL 12 IS 2 BP 403 EP 404 DI 10.1016/0888-7543(92)90392-6 PG 2 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA GY879 UT WOS:A1992GY87900029 PM 1310954 ER PT J AU COBB, RR RINCHIK, E BARNETT, LB BURKHART, JG LEWIS, SE AF COBB, RR RINCHIK, E BARNETT, LB BURKHART, JG LEWIS, SE TI A MALIC ENZYME PROBE DETECTS CROSS-HYBRIDIZING SEQUENCES CLOSELY LINKED TO LOCI ENCODING OTHER METABOLIC ENZYMES SO GENOMICS LA English DT Note ID MOUSE; ELECTROPHORESIS; IDENTIFICATION; INDUCTION; MUTANTS; MICE C1 OAK RIDGE NATL LAB,DEPT BIOL,OAK RIDGE,TN 37830. NIEHS,DEPT GENET TOXICOL,RES TRIANGLE PK,NC 27709. RP COBB, RR (reprint author), RES TRIANGLE INST,CHEM & LIFE SCI GRP,POB 12194,RES TRIANGLE PK,NC 27709, USA. NR 13 TC 3 Z9 3 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1992 VL 12 IS 2 BP 405 EP 408 DI 10.1016/0888-7543(92)90393-7 PG 4 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA GY879 UT WOS:A1992GY87900030 PM 1346782 ER PT J AU CANTOR, CR FIELDS, CA AF CANTOR, CR FIELDS, CA TI GENOME SEQUENCING CONFERENCE .3. EVOLUTION AND PROGRESS SO GENOMICS LA English DT Editorial Material C1 NIH,BETHESDA,MD 20892. RP CANTOR, CR (reprint author), UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,BERKELEY,CA 94720, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1992 VL 12 IS 2 BP 419 EP 420 DI 10.1016/0888-7543(92)90397-B PG 2 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA GY879 UT WOS:A1992GY87900034 PM 1346783 ER PT J AU SILVERMAN, DT HARTGE, P MORRISON, AS DEVESA, SS AF SILVERMAN, DT HARTGE, P MORRISON, AS DEVESA, SS TI EPIDEMIOLOGY OF BLADDER-CANCER SO HEMATOLOGY-ONCOLOGY CLINICS OF NORTH AMERICA LA English DT Article ID LOWER URINARY-TRACT; COPENHAGEN CASE-CONTROL; REDUCTION PLANT WORKERS; BLACK-WHITE DIFFERENCES; NON-HODGKINS LYMPHOMA; RISK-FACTORS; ARTIFICIAL SWEETENERS; COFFEE-DRINKING; UNITED-STATES; OCCUPATIONAL RISKS C1 BROWN UNIV,DEPT COMMUNITY HLTH,PROVIDENCE,RI 02912. RP SILVERMAN, DT (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,ROOM 415,BETHESDA,MD 20892, USA. NR 225 TC 178 Z9 183 U1 3 U2 5 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0889-8588 J9 HEMATOL ONCOL CLIN N JI Hematol. Oncol. Clin. North Am. PD FEB PY 1992 VL 6 IS 1 BP 1 EP 30 PG 30 WC Oncology; Hematology SC Oncology; Hematology GA HE469 UT WOS:A1992HE46900001 PM 1556044 ER PT J AU THOTTASSERY, J WINBERG, L YOUSSEF, J CUNNINGHAM, M BADR, M AF THOTTASSERY, J WINBERG, L YOUSSEF, J CUNNINGHAM, M BADR, M TI REGULATION OF PERFLUOROOCTANOIC ACID-INDUCED PEROXISOMAL ENZYME-ACTIVITIES AND HEPATOCELLULAR GROWTH BY ADRENAL HORMONES SO HEPATOLOGY LA English DT Article ID SEX-RELATED DIFFERENCE; RAT-LIVER PEROXISOMES; BETA-OXIDATION; HYPOLIPIDEMIC DRUGS; ZONAL HETEROGENEITY; PROLIFERATION; INDUCTION; CARCINOGENESIS; ENHANCEMENT; CLOFIBRATE AB A wide variety of compounds, including hypolipidemic drugs, plasticizers and other industrial chemicals, have been found to cause liver enlargement and hepatic peroxisome proliferation by mechanisms that are unclear. Although thyroid and sex hormones have been shown to modulate the hepatic response to these chemicals, the role of adrenal hormones in these phenomena is not clear, and a few studies have produced conflicting data. Therefore this study was undertaken to investigate the role of adrenal hormones in hepatomegaly and peroxisomal enzyme induction caused by peroxisomal proliferators and to further delineate the interrelationship between these parameters. Because adrenalectomy alters hepatic drug metabolism, we have used the nonmetabolizable proliferator perfluorooctanoic acid. Our data show that hepatomegaly caused by perfluorooctanoic acid depends on corticosterone, the major glucocorticoid in rodents. Liver growth caused by perfluorooctanoic acid appears to be predominantly hypertrophic in nature, and DNA synthesis in response to perfluorooctanoic acid predominates in periportal regions of the liver lobule. Data also show that although induction of peroxisomal beta-oxidation by perfluorooctanoic acid is independent of adrenal hormones, induction of catalase is dependent on the presence of these hormones. This study supports the contention that induction of activities of various peroxisomal enzymes is controlled by different regulatory mechanisms. C1 UNIV MISSOURI,DIV PHARMACOL,2411 HOLMES,M3-115,KANSAS CITY,MO 64108. NIEHS,EXPTL TOXICOL BRANCH,RES TRIANGLE PK,NC 27709. FU NIEHS NIH HHS [ES 04778] NR 33 TC 14 Z9 14 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD FEB PY 1992 VL 15 IS 2 BP 316 EP 322 DI 10.1002/hep.1840150223 PG 7 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA HC051 UT WOS:A1992HC05100022 PM 1735536 ER PT J AU ALTER, HJ AF ALTER, HJ TI NEW KIT ON THE BLOCK - EVALUATION OF 2ND-GENERATION ASSAYS FOR DETECTION OF ANTIBODY TO THE HEPATITIS-C VIRUS SO HEPATOLOGY LA English DT Editorial Material ID NON-B-HEPATITIS; NON-A RP ALTER, HJ (reprint author), NIH,DEPT TRANSFUS MED,IMMUNOL SECT,BETHESDA,MD 20015, USA. NR 15 TC 145 Z9 148 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD FEB PY 1992 VL 15 IS 2 BP 350 EP 351 DI 10.1002/hep.1840150228 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA HC051 UT WOS:A1992HC05100027 PM 1310478 ER PT J AU EBERHARD, JW BARR, RA AF EBERHARD, JW BARR, RA TI SAFETY AND MOBILITY OF ELDERLY DRIVERS .2. SPECIAL ISSUE PREFACE SO HUMAN FACTORS LA English DT Editorial Material C1 NIA,BETHESDA,MD 20892. RP EBERHARD, JW (reprint author), NATL HIGHWAY TRAFF SAFETY ADM,WASHINGTON,DC, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU HUMAN FACTORS SOC PI SANTA MONICA PA BOX 1369, SANTA MONICA, CA 90406 SN 0018-7208 J9 HUM FACTORS JI Hum. Factors PD FEB PY 1992 VL 34 IS 1 BP 1 EP 2 PG 2 WC Behavioral Sciences; Engineering, Industrial; Ergonomics; Psychology, Applied; Psychology SC Behavioral Sciences; Engineering; Psychology GA HL483 UT WOS:A1992HL48300001 ER PT J AU ANDERSON, WF AF ANDERSON, WF TI USES AND ABUSES OF HUMAN GENE-TRANSFER SO HUMAN GENE THERAPY LA English DT Editorial Material ID THERAPY; PROSPECTS RP ANDERSON, WF (reprint author), NHLBI,MOLEC HEMATOL BRANCH,BLDG 10,7D-18,BETHESDA,MD 20892, USA. NR 11 TC 16 Z9 16 U1 1 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD FEB PY 1992 VL 3 IS 1 BP 1 EP 2 DI 10.1089/hum.1992.3.1-1 PG 2 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA HE644 UT WOS:A1992HE64400001 PM 1562636 ER PT J AU MARTIN, M CARRINGTON, M MANN, D AF MARTIN, M CARRINGTON, M MANN, D TI A METHOD FOR USING SERUM OR PLASMA AS A SOURCE OF DNA FOR HLA TYPING SO HUMAN IMMUNOLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; AMPLIFICATION; VIRUS AB A simple method for obtaining DNA from serum and plasma is described. Using appropriate primer pairs the polymorphic segments of HLA class II genes were amplified by the polymerase chain reaction (PCR) from this DNA, and typed using allele-specific oligonucleotide hybridization. When compared with DNA obtained from peripheral blood lymphocytes, the efficiency of the PCR was only minimally compromised and could be augmented by increasing the number of amplification cycles and/or by the addition of glycerol to the reaction mixture. This method serves as a reasonable alternative when no other source of DNA is available. C1 NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,IMMUNOGENET SECT,BLDG 560,ROOM 21-78,FREDERICK,MD 21702. NCI,DIV CANC ETIOL,IMMUNOGENET SECT,FREDERICK,MD 21701. DYNCORP,PROGRAM RESOURCES INC,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD. FU NCI NIH HHS [N01-CO-74102] NR 17 TC 19 Z9 20 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PD FEB PY 1992 VL 33 IS 2 BP 108 EP 113 DI 10.1016/0198-8859(92)90060-Z PG 6 WC Immunology SC Immunology GA HL747 UT WOS:A1992HL74700005 PM 1563980 ER PT J AU CARRINGTON, M WHITE, MB DEAN, M MANN, D WARD, FE AF CARRINGTON, M WHITE, MB DEAN, M MANN, D WARD, FE TI THE USE OF DNA HETERODUPLEX PATTERNS TO MAP RECOMBINATION WITHIN THE HLA CLASS-II REGION SO HUMAN IMMUNOLOGY LA English DT Article ID DR-BETA; MUTATIONS; SEQUENCE AB Differential DNA heteroduplex patterns were used to investigate inheritance of HLA class II region genes in a family where a living related kidney transplant was under consideration. Serologic typing of the family members for HLA class I (HLA-A, B, and C) and class II (HLA-DR and DQ) alleles indicated that the patient (109) and one sibling (126) had inherited the same maternal and paternal HLA alleles. However, a strong reciprocal mixed lymphocyte response implied that these two individuals were not completely HLA identical. Serologic typing for HLA-DQ was confirmed by allele-specific oligonucleotide typing family members for HLA-DQ-alpha and beta-genes. To assess a possible nonidentical gene(s), DNA was amplified from all family members at the second exon of the DR-beta, DQ-alpha, DP-alpha, and DP-beta genes and the products were analyzed by DNA heteroduplex formation. This method showed that individuals 109 and 126 were identical at DR and DQ but differed at DP. This difference was confirmed by allele-specific oligonucleotide hybridization and indicated that 126 had inherited a recombinant maternal chromosome with a cross-over occurring in the region between the DQ-beta and DP-alpha-genes. These data demonstrate the applicability of DNA heteroduplex patterns in establishing identity-nonidentity of alleles in the major histocompatibility complex. C1 DUKE UNIV,MED CTR,DURHAM,NC 27710. RP CARRINGTON, M (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21702, USA. RI Dean, Michael/G-8172-2012 OI Dean, Michael/0000-0003-2234-0631 FU NCI NIH HHS [N01-CO-74102] NR 18 TC 20 Z9 20 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PD FEB PY 1992 VL 33 IS 2 BP 114 EP 121 DI 10.1016/0198-8859(92)90061-Q PG 8 WC Immunology SC Immunology GA HL747 UT WOS:A1992HL74700006 PM 1532959 ER PT J AU GAFFEY, MJ TRAWEEK, ST MILLS, SE TRAVIS, WD LACK, EE MEDEIROS, LJ WEISS, LM AF GAFFEY, MJ TRAWEEK, ST MILLS, SE TRAVIS, WD LACK, EE MEDEIROS, LJ WEISS, LM TI CYTOKERATIN EXPRESSION IN ADRENOCORTICAL NEOPLASIA - AN IMMUNOHISTOCHEMICAL AND BIOCHEMICAL-STUDY WITH IMPLICATIONS FOR THE DIFFERENTIAL-DIAGNOSIS OF ADRENOCORTICAL, HEPATOCELLULAR, AND RENAL-CELL CARCINOMA SO HUMAN PATHOLOGY LA English DT Article DE ADRENOCORTICAL TUMORS; CYTOKERATIN; RENAL CELL CARCINOMA; HEPATOCELLULAR CARCINOMA; IMMUNOHISTOCHEMISTRY ID INTERMEDIATE FILAMENT PROTEINS; EPITHELIAL MEMBRANE ANTIGEN; MONOCLONAL-ANTIBODIES; ADRENAL-CORTEX; PROGNOSTIC-SIGNIFICANCE; KERATIN POLYPEPTIDES; TUMORS; LIVER; IMMUNOPEROXIDASE; HISTOPATHOLOGY C1 UNIV VIRGINIA,HLTH SCI CTR,DIV SURG PATHOL,CHARLOTTESVILLE,VA 22908. NCI,BETHESDA,MD 20892. GEORGETOWN UNIV HOSP,WASHINGTON,DC 20007. CITY HOPE NATL MED CTR,DUARTE,CA 91010. RP GAFFEY, MJ (reprint author), UNIV VIRGINIA,HLTH SCI CTR,DEPT PATHOL,BOX 214,CHARLOTTESVILLE,VA 22908, USA. NR 42 TC 66 Z9 66 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD FEB PY 1992 VL 23 IS 2 BP 144 EP 153 DI 10.1016/0046-8177(92)90235-U PG 10 WC Pathology SC Pathology GA HG316 UT WOS:A1992HG31600009 PM 1371262 ER PT J AU OH, SK KIM, SH ABBRUZZI, G ADLER, WH TAUBER, AI AF OH, SK KIM, SH ABBRUZZI, G ADLER, WH TAUBER, AI TI QUANTITATIVE DIFFERENTIATION OF THE HAPTOGLOBIN-RELATED GENE-PRODUCT FROM HAPTOGLOBIN IN HUMAN PLASMA - A POSSIBLE TEST FOR TUMOR-ASSOCIATED ANTIGEN SO HYBRIDOMA LA English DT Article ID PROTEIN; EXPRESSION; ANTIBODIES; CANCER AB The haptoglobin-related gene product (HGRP) having > 90% sequence hemology with plasma haptoglobin, has recently been implicated as a tumor maker of recurrent breast cancer. Therefore, availability of a monoclonal antibody which is specific to the HGRP and ensuing ELISAs to quantitate the HGRP without cross-reactivity with plasma haptoglobin will allow determination of the clinical value of the HGRP in human plasma under various pathophysiological conditions. A peptide representing the first 34 residues of the N-terminal portion of the HRGP was synthesized and conjugated to keyhole limpet hemocyanin (KLH) to immunize Balb/c mice. Hybrid clones that produced specific antibodies to HRGP were screened with the same peptide but conjugated to bovine serum albumin (HRGP-BSA). From 12 hybridoma clones that recognized HGRP-peptide, we obtained one IgM producing clone (27.5) by limiting dilution technique that selectively reacted with HGRP-peptide with minimal cross-reactivity with haptoglobin. Using a separate monoclonal antibody, clone 21.7, which is directed to the alpha subunit of haptoglobin we developed an enzyme-linked immunosorbent assay (ELISA) to determine the quantities of the HGRP in human plasma, which will allow assessment of HGRP as a clinical marker of malignancy. C1 NATL INST SAFETY RES KOREA,SEOUL,SOUTH KOREA. BOSTON UNIV,SCH MED,DEPT MED,BOSTON,MA 02118. BOSTON UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02118. NIH,CLIN IMMUNOL SECT,BETHESDA,MD 20892. RP OH, SK (reprint author), BOSTON UNIV,SCH MED,DEPT BIOCHEM,ROOM S-302,80 E CONCORD ST,BOSTON,MA 02118, USA. NR 28 TC 4 Z9 4 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0272-457X J9 HYBRIDOMA JI Hybridoma PD FEB PY 1992 VL 11 IS 1 BP 1 EP 12 DI 10.1089/hyb.1992.11.1 PG 12 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Immunology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology GA HW515 UT WOS:A1992HW51500001 PM 1737636 ER PT J AU METZGER, H AF METZGER, H TI THE RECEPTOR WITH HIGH-AFFINITY FOR IGE SO IMMUNOLOGICAL REVIEWS LA English DT Review ID BASOPHILIC LEUKEMIA-CELLS; RBL-2H3 MAST-CELLS; IMMUNOGLOBULIN-E; HISTAMINE-RELEASE; SIGNAL TRANSDUCTION; TRANSFECTED CELLS; CROSS-LINKING; ALPHA-SUBUNIT; FC-RECEPTORS; ZETA-CHAIN RP METZGER, H (reprint author), NIAMSD,ARTHRITIS & RHEUMATISM BRANCH,BETHESDA,MD 20892, USA. NR 60 TC 197 Z9 198 U1 0 U2 2 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0105-2896 J9 IMMUNOL REV JI Immunol. Rev. PD FEB PY 1992 VL 125 BP 37 EP 48 DI 10.1111/j.1600-065X.1992.tb00624.x PG 12 WC Immunology SC Immunology GA HD828 UT WOS:A1992HD82800003 PM 1532373 ER PT J AU XU, R BURDICK, JF SCOTT, A BESCHORNER, WE ADLER, W KITTUR, DS AF XU, R BURDICK, JF SCOTT, A BESCHORNER, WE ADLER, W KITTUR, DS TI GRAFT-SPECIFIC MHC CLASS-II GENE-EXPRESSION IN RESPONSE TO ALLOGENEIC STIMULUS IN HETEROTOPIC MURINE CARDIAC ALLOGRAFTS SO IMMUNOLOGY LA English DT Article ID COMPLEX CLASS-I; HISTOCOMPATIBILITY COMPLEX; A-BETA; HOST-DISEASE; TUMOR-CELLS; ANTIGENS; INDUCTION; UNRESPONSIVENESS; KERATINOCYTES; POLYMORPHISM AB Although, major histocompatibility complex (MHC) class II antigen expression in allografts is thoroughly studied, regulation of the genes for these antigens is not fully understood. The graft-specific MHC class II genes are potentially important in determining the immunogenicity of graft but their detection in a mixed-cell population such as in the allograft would require unambiguous differentiation of graft-specific class II expression from those in host lymphoid cells. With an oligonucleotide probe that specifically hybridizes to I-Ab mRNA from H-2k haplotype mice, we have studied I-A gene expression in cardiac allografts heterotopically transplanted from B10.Br (H-2k) to B10.D2 (H-2d) mice. Normal B10.Br hearts do not have appreciable I-Ab transcripts as determined with this probe, but 4 days after allografting, a substantial increase in I-Ab(k) messenger RNA (mRNA) content was noted in the allografted hearts which persisted for the next 2 days and then decreased concomitant with destruction of the heart. The increase in I-Ab(k) mRNA preceded the expression of surface Ia(k) antigens on dendritic and endothelial cells in the allograft. These data indicate increased transcription of the I-Ab gene in cells of graft origin suggesting that transcriptional regulation is the initial mechanism for expression of class II genes in allografts. The sustained rise in graft-specific class II mRNA also seen in these allografts suggests that increased mRNA stability may be another mechanism for the increased density of class II antigens in allografts undergoing rejection. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT SURG,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT MED GENET,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,BALTIMORE,MD 21205. NIA,FRANCIS SCOTT KEY MED CTR,GERONTOL RES CTR,CLIN IMMUNOL SECT,BALTIMORE,MD 21224. FU NCI NIH HHS [CA28701]; NIAID NIH HHS [AI25550, AI15081] NR 30 TC 7 Z9 7 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0019-2805 J9 IMMUNOLOGY JI Immunology PD FEB PY 1992 VL 75 IS 2 BP 361 EP 365 PG 5 WC Immunology SC Immunology GA HE028 UT WOS:A1992HE02800025 PM 1551698 ER PT J AU SADEGHNASSERI, S GERMAIN, RN AF SADEGHNASSERI, S GERMAIN, RN TI HOW MHC CLASS-II MOLECULES WORK - PEPTIDE-DEPENDENT COMPLETION OF PROTEIN FOLDING SO IMMUNOLOGY TODAY LA English DT Article ID INVARIANT CHAIN; BINDING-SITE; ANTIGEN; IA; ALLOANTIGENS; DISSOCIATION; RECOGNITION; ASSOCIATION; COMPLEXES AB It is now clear that peptides play a key role in stabilizing the structure of MHC class II molecules. Here, Scheherazade Sadegh-Nasseri and Ron Germain propose that newly synthesized MHC class II, and indeed class I, molecules behave like partially folded proteins, with peptides acting as a surrogate portion of the MHC polypeptide structure that is necessary for completion of conformational maturation. RP SADEGHNASSERI, S (reprint author), NIAID,IMMUNOL LAB,LYMPHOCYTE BIOL SECT,BETHESDA,MD 20892, USA. OI Sadegh-Nasseri, Scheherazade/0000-0002-8127-1720 NR 26 TC 66 Z9 66 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0167-5699 J9 IMMUNOL TODAY JI Immunol. Today PD FEB PY 1992 VL 13 IS 2 BP 43 EP 46 DI 10.1016/0167-5699(92)90131-P PG 4 WC Immunology SC Immunology GA HD549 UT WOS:A1992HD54900002 PM 1533524 ER PT J AU KEEGAN, AD PAUL, WE AF KEEGAN, AD PAUL, WE TI MULTICHAIN IMMUNE RECOGNITION RECEPTORS - SIMILARITIES IN STRUCTURE AND SIGNALING PATHWAYS SO IMMUNOLOGY TODAY LA English DT Review ID CELL ANTIGEN RECEPTOR; PROTEIN TYROSINE PHOSPHORYLATION; NATURAL-KILLER-CELLS; FC-GAMMA RECEPTOR; B-CELLS; CYCLOSPORIN-A; MESSENGER-RNA; IGE RECEPTOR; ZETA-CHAIN; MAST-CELLS AB Cells involved in immune recognition bear characteristic and complex multichain receptors. Here, Achsah Keegan and William Paul propose, based on familial relationships in both extracellular and intracellular domains, that they be grouped in a set, designated the multichain immune recognition receptors. RP KEEGAN, AD (reprint author), NIAID,IMMUNOL LAB,BETHESDA,MD 20892, USA. NR 76 TC 182 Z9 183 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0167-5699 J9 IMMUNOL TODAY JI Immunol. Today PD FEB PY 1992 VL 13 IS 2 BP 63 EP 68 DI 10.1016/0167-5699(92)90136-U PG 6 WC Immunology SC Immunology GA HD549 UT WOS:A1992HD54900008 PM 1575894 ER PT J AU FRYLING, C OGATA, M FITZGERALD, D AF FRYLING, C OGATA, M FITZGERALD, D TI CHARACTERIZATION OF A CELLULAR PROTEASE THAT CLEAVES PSEUDOMONAS EXOTOXIN SO INFECTION AND IMMUNITY LA English DT Article ID RECEPTOR-MEDIATED ENTRY; PROTEOLYTIC CLEAVAGE; AERUGINOSA; TOXIN; EXPRESSION; DELETION; FRAGMENT; MUTANTS; CELLS AB Pseudomonas exotoxin (PE) is a 66-kDa bacterial toxin that is proteolytically cleaved by cells to produce an N-terminal fragment of 28 kDa and a C-terminal 37-kDa fragment which translocates to the cytosol and inhibits protein synthesis (M. Ogata, V. K. Chaudhary, I. Pastan, and D. J. FitzGerald, J. Biol. Chem. 265:20678-20685, 1990). When cells were broken by homogenization, the appropriate proteolytic activity was found associated with cellular membranes and not in a soluble fraction. Proteolysis of PE by crude membranes was stimulated by divalent cations, was ATP independent, and had a pH optimum of 5.5. When cells were disrupted by nitrogen cavitation and fractionated on Percoll gradients, proteolytic activity was present in fractions corresponding to the density of plasma membranes or endosomes but not in fractions containing lysosomes. Proteolytic activity was recovered in detergent extracts after crude membranes were treated with Nonidet P-40 or octylglucoside. Proteolysis of PE by either crude membranes or detergent extracts generated fragments of 28 and 37 kDa. The sizes of these fragments resembled those produced by intact cells. However, when the nontoxic mutant, PEgly276, which cannot be cleaved appropriately by intact cells, was incubated with membranes or extracts there was no production of the 28- and 37-kDa fragments. C1 NCI,DIV CANC BIOL DIAG,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 25 TC 42 Z9 43 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1992 VL 60 IS 2 BP 497 EP 502 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA HA564 UT WOS:A1992HA56400025 PM 1730481 ER PT J AU FATTOM, A SHILOACH, J BRYLA, D FITZGERALD, D PASTAN, I KARAKAWA, WW ROBBINS, JB SCHNEERSON, R AF FATTOM, A SHILOACH, J BRYLA, D FITZGERALD, D PASTAN, I KARAKAWA, WW ROBBINS, JB SCHNEERSON, R TI COMPARATIVE IMMUNOGENICITY OF CONJUGATES COMPOSED OF THE STAPHYLOCOCCUS-AUREUS TYPE-8 CAPSULAR POLYSACCHARIDE BOUND TO CARRIER PROTEINS BY ADIPIC ACID DIHYDRAZIDE OR N-SUCCINIMIDYL-3-(2-PYRIDYLDITHIO)PROPIONATE SO INFECTION AND IMMUNITY LA English DT Article ID AERUGINOSA EXOTOXIN-A; INFLUENZAE TYPE-B; PSEUDOMONAS-AERUGINOSA; ANTIBODY-RESPONSE; TOXIN; PREDOMINANCE; HEMODIALYSIS; SEPTICEMIA; BACTEREMIA; VACCINES AB Staphylococcus aureus type 8 capsular polysaccharide (CP) was conjugated either to diphtheria toxoid or to Pseudomonas aeruginosa recombinant exoprotein A by using adipic acid dihydrazide (ADH) or N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) as the joining reagent. The polysaccharide/protein ratios of these two pairs of conjugates were similar. The two synthetic schemes bound the linker to the carboxyls of the type 8 CP by carbodiimide-mediated condensation. ADH was bound to the carboxyls of the protein, whereas SPDP reacted with the amino groups of the protein. Intermolecular linking of the carrier protein, caused by the carbodiimide during the conjugation reaction with the type 8 CP derivative, probably accounts for the larger size of the conjugates formed with ADH compared with those formed with SPDP. Both conjugates synthesized with ADH elicited higher levels of CP antibodies, especially after the first immunization, than did those prepared with SPDP. Similar levels of exoprotein A antibodies were elicited by both conjugates. Higher levels of diphtheria toxoid antibodies were elicited by the conjugate prepared with SPDP than by the one prepared with ADH. The basis for the differences in the immunogenicities of these two pairs of S. aureus type 8 CP conjugates is discussed. C1 NICHHD,DEV & MOLEC IMMUN LAB,BETHESDA,MD 20892. NICHHD,BIOMETRY & MATH STAT BRANCH,BETHESDA,MD 20892. NIDDKD,BIOTECHNOL UNIT,BETHESDA,MD 20892. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. PENN STATE UNIV,DEPT BIOCHEM,UNIV PK,PA 16802. NR 38 TC 39 Z9 40 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1992 VL 60 IS 2 BP 584 EP 589 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA HA564 UT WOS:A1992HA56400038 PM 1730492 ER PT J AU EMANCIPATOR, K CSAKO, G ELIN, RJ AF EMANCIPATOR, K CSAKO, G ELIN, RJ TI INVITRO INACTIVATION OF BACTERIAL-ENDOTOXIN BY HUMAN LIPOPROTEINS AND APOLIPOPROTEINS SO INFECTION AND IMMUNITY LA English DT Article ID HIGH-DENSITY LIPOPROTEINS; SALMONELLA-TYPHIMURIUM LIPOPOLYSACCHARIDES; LIMULUS LYSATE; BINDING; SERUM; RAT; NEUTRALIZATION; INVIVO; PLASMA AB A chromogenic Limulus amebocyte lysate assay was used to measure the recovery of 1 endotoxin unit of endotoxin per ml. Purified human high-density lipoprotein, low-density lipoprotein, and apolipoprotein A1 (apo A1) at a maximum concentration of 1 g of protein per liter reduced the recovery to < 40% of baseline in a both dose- and time-dependent manner and in the absence of other serum components. Furthermore, the lapine fever response to a dose of 1 ml of 5-ng/ml endotoxin per kg was reduced by > 0.5-degrees-C (P < 0.005) when the solution was preincubated in vitro with 0.5 g of apo A1 per liter. By the Limulus test, a maximum concentration of 0.01 g of apolipoprotein B (apo B) per liter (which contained deoxycholate, a known endotoxin-disaggregating agent) reduced recovery to 0% in a dose- but not time-dependent manner. In heat-inactivated (56-degrees-C, 1 h) normal human serum, high-density lipoprotein cholesterol (P < 0.005) and apo A1 (P < 0.05) correlated inversely with endotoxin recovery, but, paradoxically, apo B correlated directly with endotoxin recovery (P < 0.05), while low-density lipoprotein cholesterol showed no significant correlation. INTRALIPID alone had no effect on endotoxin recovery. Addition of a maximum of 10 g of INTRALIPID per liter to 0.0042 g of apo B per liter increased endotoxin recovery from approximately 30 to 80% (P < 0.001), but addition of INTRALIPID to 0.25 g of apo A1 per liter decreased recovery from approximately 30 to 20% (P < 0.001). We conclude that (i) lipoproteins are endotoxin inactivators; (ii) this ability of lipoproteins may be modulated by their lipid component (lipid-endotoxin interaction); (iii) apo A1 is capable of directly inactivating endotoxin (protein-endotoxin interaction). RP EMANCIPATOR, K (reprint author), NIH,WARREN G MAGNUSON CLIN CTR,DEPT CLIN PATHOL,BETHESDA,MD 20892, USA. NR 26 TC 112 Z9 112 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1992 VL 60 IS 2 BP 596 EP 601 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA HA564 UT WOS:A1992HA56400040 PM 1730494 ER PT J AU KWONCHUNG, KJ EDMAN, JC WICKES, BL AF KWONCHUNG, KJ EDMAN, JC WICKES, BL TI GENETIC ASSOCIATION OF MATING TYPES AND VIRULENCE IN CRYPTOCOCCUS-NEOFORMANS SO INFECTION AND IMMUNITY LA English DT Article ID HISTOPLASMA-CAPSULATUM; FILOBASIDIELLA; ALPHA AB A pair of congenic Cryptococcus neoformans var. neoformans strains, B-4476 (a mating type) and B-4500 (alpha-mating type), that presumably differ only in mating type was constructed. This pair and their progeny, five alpha-type and five a type, were tested for virulence in mice. In the parent strains as well as the progeny, alpha-type was clearly more virulent than a type. In addition, death tended to occur earlier among the alpha-strain-infected mice that died than among the mice that died by infection caused by a strains. These data strongly suggest the genetic association of virulence with mating type in this human fungal pathogen. C1 UNIV CALIF SAN FRANCISCO,HORMONE RES INST,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143. RP KWONCHUNG, KJ (reprint author), NIAID,CLIN INVEST LAB,CLIN MYCOL SECT,BETHESDA,MD 20892, USA. FU NIAID NIH HHS [R01 AI29312] NR 21 TC 313 Z9 322 U1 2 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1992 VL 60 IS 2 BP 602 EP 605 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA HA564 UT WOS:A1992HA56400041 PM 1730495 ER PT J AU PICHOVA, I STROP, P SEDLACEK, J KAPRALEK, F BENES, V TRAVNICEK, M PAVLICKOVA, L SOUCEK, M KOSTKA, V FOUNDLING, S AF PICHOVA, I STROP, P SEDLACEK, J KAPRALEK, F BENES, V TRAVNICEK, M PAVLICKOVA, L SOUCEK, M KOSTKA, V FOUNDLING, S TI ISOLATION, BIOCHEMICAL-CHARACTERIZATION AND CRYSTALLIZATION OF THE P15GAG PROTEINASE OF MYELOBLASTOSIS ASSOCIATED VIRUS EXPRESSED IN ESCHERICHIA-COLI SO INTERNATIONAL JOURNAL OF BIOCHEMISTRY LA English DT Article ID ROUS-SARCOMA VIRUS; ESCHERICHIA-COLI; STRUCTURAL PROTEINS; PROTEASE; PURIFICATION; PRECURSOR; SEQUENCE AB 1. The p15gag proteinase responsible for the processing of the polyprotein precursor of the myeloblastosis associated virus was obtained by a recombinant technique in an E. coli expression system. The massive expression of the intentionally truncated precursor (Pr25lac-DELTA-gag) was accompanied by its structurally correct processing. 2. Three procedures for the purification of the recombinant proteinase from both the cytoplasmic fraction and the inclusion bodies were developed. 3. The purified proteinase was compared with the authentic proteinase isolated from MAV virions by N-terminal sequence analysis and amino acid analysis, molecular weight determination, reverse-phase HPLC and FPLC elution profiles, electrophoretic mobility and isoelectric point determination, and activity assays with proteins and synthetic substrates. The identity of both enzymes was shown. 3. Contrary to reported data, the amino acid sequence of the p15gag proteinase differs from the sequence of the homologous Rous sarcoma virus proteinase in one residue only, as follows from cDNA sequencing. 4. Crystallization of the proteinase from a citrate-phosphate buffer at pH 5.6 afforded hexagonal crystals which diffracted well at 2.3 angstrom without deterioration. C1 CZECHOSLOVAK ACAD SCI,INST MOLEC GENET,CS-16637 PRAGUE,CZECHOSLOVAKIA. NATL CANC RES INST,FREDERICK CANC RES FACIL,FREDERICK,MD. RP PICHOVA, I (reprint author), CZECHOSLOVAK ACAD SCI,INST ORGAN CHEM & BIOCHEM,CS-16610 PRAGUE 6,CZECHOSLOVAKIA. RI Pichova, Iva/G-7917-2014; Sedlacek, Juraj/G-3606-2014 NR 24 TC 9 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0020-711X J9 INT J BIOCHEM JI Int. J. Biochem. PD FEB PY 1992 VL 24 IS 2 BP 235 EP 242 DI 10.1016/0020-711X(92)90252-V PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA GU779 UT WOS:A1992GU77900008 PM 1733789 ER PT J AU CHANG, K PASTAN, I WILLINGHAM, MC AF CHANG, K PASTAN, I WILLINGHAM, MC TI ISOLATION AND CHARACTERIZATION OF A MONOCLONAL-ANTIBODY, K1, REACTIVE WITH OVARIAN CANCERS AND NORMAL MESOTHELIUM SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID TUMOR-ASSOCIATED ANTIGENS; CELL-SURFACE ANTIGEN; CARCINOMA; IMMUNOTOXINS; SPECIFICITY AB We have isolated a new monoclonal antibody (MAb), K1, that reacts with an epitope on the surface of human ovarian carcinoma cells. This antibody was generated by immunization of mice with periodate-treated human ovarian carcinoma (OVCAR-3) cells. These mice had been previously made tolerant with normal human kidney membranes. Spleen lymphocytes from these mice were selected prior to fusion using a panning purification method on living OVCAR-3 cells. Initial screening of surface-reactive clones was performed in a single day using immunofluorescence on living OVCAR-3 cells, and secondary screening was performed using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. The K1 clone was subcloned and identified as an IgM isotype, but was subsequently isotype-switched to IgG1K using a panning selection method. When evaluated by immunohistochemistry, the antigen reactive with K1 was found in many ovarian non-mucinous tumors, as well as in squamous tumors of the esophagus, and cervical cancer. The only normal adult human tissues showing uniform reactivity with K1 were the mesothelia of the peritoneal, pleural and pericardial cavities. There was also limited reactivity with epithelia of the trachea, tonsil and Fallopian tube. A similar tissue reactivity for K1 was found in tissues from cynomolgus monkeys, K1 reacted with many of the same tissues and tumors as the previously identified antibody OC125, but several lines of evidence indicate that K1 reacts with a different epitope and probably a different molecule, when compared to OC125. This evidence included assays employing immunofluorescence competition, double-label immunofluorescence, and solid-phase and live-cell radioimmunoassays. Since our data indicate that the antigen reactive with the K1 antibody is a new molecular species, we have named the antigen CAK1. Unlike the shed antigen CA125, CAK1 was only cell-associated and was not found in the supernatant of cultured OVCAR-3 cells or in the blood of ovarian cancer patients. The K1 antibody may be useful as a targeting agent for therapy and in the diagnosis of ovarian carcinoma, as well as some other human cancers. RP CHANG, K (reprint author), NCI,DIV CANC BIOL,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 26 TC 130 Z9 131 U1 0 U2 6 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD FEB 1 PY 1992 VL 50 IS 3 BP 373 EP 381 DI 10.1002/ijc.2910500308 PG 9 WC Oncology SC Oncology GA HC030 UT WOS:A1992HC03000007 PM 1735605 ER PT J AU CHEN, Y WU, PC LANG, JH GE, WJ HARTGE, P BRINTON, LA AF CHEN, Y WU, PC LANG, JH GE, WJ HARTGE, P BRINTON, LA TI RISK-FACTORS FOR EPITHELIAL OVARIAN-CANCER IN BEIJING, CHINA SO INTERNATIONAL JOURNAL OF EPIDEMIOLOGY LA English DT Article ID MUMPS; CARCINOMA; MENOPAUSE; EVENTS AB A study in Beijing, China of 112 pathologically confirmed epithelial ovarian cancer cases and 224 age-matched community controls enabled evaluation of risk in relation to reproductive, medical, familial, and selected lifestyle factors. An inverse relationship was observed between the number of full-term pregnancies and ovarian cancer risk. Compared to nulliparous women, subjects with one, two, or three full-term pregnancies were at 50%, 70%, or 90% reduced risks, respectively (P for trend < 0.01). A positive correlation was found between the number of ovulatory years and risk, with a 2.6-fold increased risk for women with 30 or more compared to less than 10 ovulatory years (P for trend < 0.01). Infertility, as estimated in various ways, was also found to be an important risk factor. When parity was taken into account, age at first pregnancy was not related to ovarian cancer risk. No protective effect was associated with mumps virus infection. In contrast, risk increased significantly as serum mumps virus antibody titres increased (P for trend < 0.01). An elevated risk was found in women with a history of long-term (> 3 months) application of talc-containing dusting powder to the lower abdomen and perineum (Relative risk 3.9, 95% confidence interval: 0.9-10.63). These findings suggest that Chinese women have risk factors similar to those of occidental women. C1 NCI,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,RM 443,BETHESDA,MD 20892. BEIJING UNION MED COLL,DEPT OBSTET & GYNAECOL,BEIJING,PEOPLES R CHINA. BEIJING UNION MED COLL,DEPT BIOSTAT,BEIJING,PEOPLES R CHINA. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 27 TC 82 Z9 84 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0300-5771 J9 INT J EPIDEMIOL JI Int. J. Epidemiol. PD FEB PY 1992 VL 21 IS 1 BP 23 EP 29 DI 10.1093/ije/21.1.23 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA HF453 UT WOS:A1992HF45300004 PM 1544753 ER PT J AU FORMAN, MR LEWANDOHUNDT, G GRAUBARD, BI CHANG, D SAROV, B NAGGAN, L BERENDES, HW AF FORMAN, MR LEWANDOHUNDT, G GRAUBARD, BI CHANG, D SAROV, B NAGGAN, L BERENDES, HW TI FACTORS INFLUENCING MILK INSUFFICIENCY AND ITS LONG-TERM HEALTH-EFFECTS - THE BEDOUIN INFANT-FEEDING STUDY SO INTERNATIONAL JOURNAL OF EPIDEMIOLOGY LA English DT Article AB Women who breastfeed have frequently reported milk insufficiency as the reason for introducing the bottle, but no one has addressed its potential long-term health effects. This paper described the factors associated with milk insufficiency versus another reason for introducing the bottle and its potential health effects based on an analysis of a prospective cohort study of 1005 Bedouin Arab women who delivered healthy newborns in 1981 and 1982. By two months postpartum, 72% introduced the infant to the bottle with 72% reporting milk insufficiency as the reason for introducing the bottle. The percentage of milk insufficiency declined with increasing age of the infant. Based on multiple logistic regression analyses, birth season was statistically significantly associated with the odds ratio (OR) of milk insufficiency versus another reason for introducing the bottle during the first two months. Women who delivered in the spring-summer had an increased OR = 1.65 of reported milk insufficiency compared with those who delivered during the rest of the year. Parity was directly related to the OR = 1.04 of milk insufficiency (but just missed significance) during one to two months and was statistically significantly associated with the OR = 1.12 of reported milk insufficiency during 3-18 months. The rates of stunting after the infant was introduced to the bottle and the duration of breastfeeding did not differ by reason for introducing the bottle. Thus the high frequency of reported milk insufficiency was not associated with adverse health effects. RP FORMAN, MR (reprint author), NCI,DCPC,CANC PREVENT STUDIES BRANCH,EPN-211C,BETHESDA,MD 20892, USA. NR 13 TC 12 Z9 12 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0300-5771 J9 INT J EPIDEMIOL JI Int. J. Epidemiol. PD FEB PY 1992 VL 21 IS 1 BP 53 EP 58 DI 10.1093/ije/21.1.53 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA HF453 UT WOS:A1992HF45300009 PM 1544758 ER PT J AU WILLIAMS, AO HUGGETT, AC THORGEIRSSON, SS AF WILLIAMS, AO HUGGETT, AC THORGEIRSSON, SS TI PATHOLOGY OF SPONTANEOUS AND ONCOGENE TRANSFORMED RAT-LIVER EPITHELIAL-CELLS AND DERIVED TUMORS IN NUDE-MICE SO INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY LA English DT Article DE RAT LIVER; ONCOGENE; EPITHELIAL CELLS; TRANSFORMATION; MORPHOLOGY; TUMOR ID C-MYC ONCOGENE; HEPATOCELLULAR-CARCINOMA; NEOPLASTIC TRANSFORMATION; TRANSGENIC MICE; OVAL CELLS; V-RAF; EXPRESSION; HEPATOBLASTOMAS; DIFFERENTIATION; TUMORIGENICITY AB The histological and ultrastructural features of oncogene transformed rat liver epithelial (RLE) cells in culture and spontaneously transformed RLE cells were studied. The tumours produced in nude mice by all the transformed cells were either moderately well differentiated carcinomas or sarcomatous tumours. Epithelial tumours were produced in the RLE cells after transduction of both v-raf and v-myc oncogenes whereas sarcomatous tumours were produced after transduction of v-raf alone. These data suggested that transformation of RLE cells with a single oncogene, v-raf, produced malignant tumours which were consistent with sarcomas while a combination of two oncogenes, v-raf and v-myc, produced an epithelial tumour, consistent with a carcinoma. The effects of these oncogenes on RLE cells indicate that they were able to differentiate and were capable of producing two morphologically distinct tumour types. The possible role of v-myc in switching the sarcomatous lineage to an epithelial tumour lineage is considered to be significant and worthy of further studies. The epithelial tumour produced in RLE cells by combination of v-raf and v-myc is consistent with an embryonal type of hepatoblastoma. The trabecular type of liver cell carcinoma which resulted from spontaneous transformation of RLE cells illustrates the inherent potential of the RLE cell to undergo malignant change and strongly suggests that the RLE cells may be the precursor cells in the development of hepatocellular carcinoma in the rat. C1 NCI,DIV CANC ETIOL,CHEM & PHYS CARCINOGENESIS PROGRAM,BETHESDA,MD 20892. NIH,FOGARTY INT CTR,BETHESDA,MD 20892. NR 33 TC 16 Z9 16 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0959-9673 J9 INT J EXP PATHOL JI Int. J. Exp. Pathol. PD FEB PY 1992 VL 73 IS 1 BP 99 EP 114 PG 16 WC Pathology SC Pathology GA HD582 UT WOS:A1992HD58200012 PM 1576082 ER PT J AU CORSINI, E CRAIG, WA ROSENTHAL, GJ AF CORSINI, E CRAIG, WA ROSENTHAL, GJ TI MODULATION OF TUMOR-NECROSIS-FACTOR RELEASE FROM ALVEOLAR MACROPHAGES TREATED WITH PENTAMIDINE ISETHIONATE SO INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY LA English DT Article ID PNEUMOCYSTIS-CARINII PNEUMONIA; THERAPEUTIC USE; FACTOR-ALPHA; LUNG; PATHOGENESIS; AIDS AB Alveolar macrophages in AIDS patients have a marked increase in tumor necrosis factor release in active Pneumocystis carinii pneumonia. We have demonstrated that pentamidine, an aromatic diamidine currently used to treat AIDS-related P. carinii pneumonia, is an effective inhibitor of cellular tumor necrosis factor release from lipopolysaccharide-stimulated rat alveolar macrophages at concentrations > 10(-8) M. Inhibition of release is not dependent upon the continued presence of pentamidine in the culture medium during the release phase. In addition, this blockage occurs at neither the transcriptional level as determined by Northern blot analysis nor the translational level as determined by Western blot analysis. Timed addition studies suggest that pentamidine is targeting relatively early events following lipopolysaccharide administration. Pentamidine appears to alter early lipopolysaccharide-induced cellular processes associated with the release of tumor necrosis factor from macrophages. C1 NIEHS,DIV TOXICOL RES & TESTING,IMMUNOTOXICOL SECT,POB 12233,RES TRIANGLE PK,NC 27709. UNIV MILAN,INST PHARMACOL SCI,I-20122 MILAN,ITALY. RI Corsini, Emanuela/B-5602-2011 NR 29 TC 16 Z9 16 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0192-0561 J9 INT J IMMUNOPHARMACO JI Int. J. Immunopharmacol. PD FEB PY 1992 VL 14 IS 2 BP 121 EP 130 DI 10.1016/0192-0561(92)90022-D PG 10 WC Immunology; Pharmacology & Pharmacy SC Immunology; Pharmacology & Pharmacy GA HF986 UT WOS:A1992HF98600002 PM 1624213 ER PT J AU SHTERN, F AF SHTERN, F TI POSITRON EMISSION TOMOGRAPHY AS A DIAGNOSTIC-TOOL - A REASSESSMENT BASED ON LITERATURE-REVIEW SO INVESTIGATIVE RADIOLOGY LA English DT Article DE FLUORODEOXYGLUCOSE; METABOLIC IMAGING; NONINVASIVE TISSUE CHARACTERIZATION; POSITRON EMISSION TOMOGRAPHY; POSITRON EMISSION TOMOGRAPHY-FLUORODEOXYGLUCOSE ID F-18 FLUORODEOXYGLUCOSE; RADIATION NECROSIS; CEREBRAL GLIOMAS; WALL-MOTION; PERFUSION; PET; BRAIN; METABOLISM; TUMORS; TISSUE AB Currently used clinical diagnostic imaging modalities, such as magnetic resonance imaging (MRI) and x-ray computed tomography (CT) provide predominantly anatomic information. CT images reflect x-ray attenuation distribution in the body, whereas MRI signals depend primarily on proton density and tissue relaxivity. In contrast to these predominantly anatomic modalities, positron emission tomography (PET) reflects tissue physiology and metabolism. Although PET has been used predominantly as a research tool, the clinical use of this technique for the detection, noninvasive characterization, and treatment planning of selected disease processes has been extensively studied in oncology, cardiology, and neurology. The author examined currently available literature to reassess the potential role of PET as a diagnostic tool in the following specific clinical situations: (1) the differentiation of radiation necrosis from tumor recurrence; (2) the characterization of the physiologic significance of coronary stenosis and the evaluation of the myocardial viability; and (3) the localization of the epileptogenic foci. RP SHTERN, F (reprint author), NCI,DIV CANC TREATMENT,DIAGNOST IMAGING RES BRANCH,RADIAT RES PROGRAM,6130 EXECUT BLVD,SUITE 800,ROCKVILLE,MD 20852, USA. NR 24 TC 10 Z9 10 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0020-9996 J9 INVEST RADIOL JI Invest. Radiol. PD FEB PY 1992 VL 27 IS 2 BP 165 EP 168 DI 10.1097/00004424-199202000-00015 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA HC523 UT WOS:A1992HC52300012 PM 1601609 ER PT J AU VANDEWOUDE, GF AF VANDEWOUDE, GF TI HEPATOCYTE GROWTH-FACTOR - MITOGEN, MOTOGEN, AND MORPHOGEN SO JAPANESE JOURNAL OF CANCER RESEARCH LA English DT Editorial Material ID MOLECULAR-CLONING; PRIMARY CULTURE; PURIFICATION RP VANDEWOUDE, GF (reprint author), FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702, USA. NR 33 TC 0 Z9 0 U1 0 U2 1 PU JAPANESE CANCER ASSOCIATION PI TOKYO PA EDITORIAL OFFICE 7TH FLOOR, JOHKOH BLDG 2-23-11, KOISHIKAWA, TOKYO 112, JAPAN SN 0910-5050 J9 JPN J CANCER RES JI Jpn. J. Cancer Res. PD FEB PY 1992 VL 83 IS 2 BP U127 EP U127 PG 1 WC Oncology SC Oncology GA HE358 UT WOS:A1992HE35800001 ER PT J AU MCGUE, M PICKENS, RW SVIKIS, DS AF MCGUE, M PICKENS, RW SVIKIS, DS TI SEX AND AGE EFFECTS ON THE INHERITANCE OF ALCOHOL-PROBLEMS - A TWIN STUDY SO JOURNAL OF ABNORMAL PSYCHOLOGY LA English DT Article ID CROSS-FOSTERING ANALYSIS; ANTISOCIAL PERSONALITY; FAMILIAL TRANSMISSION; PSYCHIATRIC-DISORDERS; ABUSE; MEN; EXPECTANCIES; DEPRESSION; GENETICS; HISTORY AB Male monozygotic cotwins of probands with Alcohol Abuse-Dependence (n = 85) were more likely than male same-sex dizygotic cotwins (n = 96) to report alcohol, drug, and conduct disorder problems. For women, rates of problem behavior did not differ between monozygotic (n = 44) and same-sex dizygotic (n = 43) cotwins. Opposite-sex dizygotic twin data (n = 88) revealed significant cross-sex transmission; alcohol problems were greatest among male cotwins of female probands. For men, proportion of liability variance associated with additive genetic factors was significantly greater when proband had an early (h2 = .73 +/- .18) rather than late (h2 = .30 +/- .26) age of onset. For women, heritability did not vary as a function of proband's age of onset, and the pooled estimate suggested little genetic influence (h2 = .00, SE not computable). Findings suggest that genetic influences may be substantial only in the etiology of early-onset male alcoholism. C1 NIDA,ADDICT RES CTR,WASHINGTON,DC. JOHNS HOPKINS UNIV,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21218. RP MCGUE, M (reprint author), UNIV MINNESOTA,DEPT PSYCHOL,75 E RIVER RD,MINNEAPOLIS,MN 55455, USA. FU NIA NIH HHS [AG06886]; NIAAA NIH HHS [AA06500]; NIDA NIH HHS [DA05147] NR 59 TC 189 Z9 193 U1 6 U2 9 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0021-843X J9 J ABNORM PSYCHOL JI J. Abnorm. Psychol. PD FEB PY 1992 VL 101 IS 1 BP 3 EP 17 DI 10.1037/0021-843X.101.1.3 PG 15 WC Psychology, Clinical; Psychology, Multidisciplinary SC Psychology GA GY962 UT WOS:A1992GY96200001 PM 1537970 ER PT J AU AFESSA, B GREAVES, W GREEN, W OLOPOENIA, L DELAPENHA, R SAXINGER, C FREDERICK, W AF AFESSA, B GREAVES, W GREEN, W OLOPOENIA, L DELAPENHA, R SAXINGER, C FREDERICK, W TI AUTOPSY FINDINGS IN HIV-INFECTED INNER-CITY PATIENTS SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE HIV-1 INFECTION; AUTOPSY; INNER-CITY PATIENTS ID ACQUIRED IMMUNODEFICIENCY SYNDROME; IMMUNE-DEFICIENCY SYNDROME; TESTICULAR ATROPHY; SYNDROME AIDS; PREMORTEM; PNEUMONIA; NECROPSY AB To assess the importance of the autopsy in HIV-1 infection, we retrospectively reviewed the autopsy reports of 70 HIV-1-seropositive patients at Howard University Hospital. Of the 58 patients with AIDS, the diagnosis of AIDS was made after autopsy in 24 (41%) cases. The lung was the most common site of AIDS-diagnostic diseases, and was affected in 90% of patients. Pneumocystis carinii infection was the most common AIDS-diagnostic disease, and was present in 50% of the AIDS patients. Thirty-eight percent of AIDS diagnostic diseases were diagnosed antemortem, including 15 of the 29 Pneumocystis carinii infections. Most of the AIDS-diagnostic diseases were disseminated at autopsy and two or more diseases were found in some organs. Overall, Pneumocystis carinii pneumonia was the most common cause of death, accounting for a mortality of 43% among AIDS patients. Bacterial infections were common and contributed to the mortality and morbidity of both AIDS and non-AIDS patients. Bacterial infection was the cause of death in 15 AIDS and 9 non-AIDS patients. The clinical cause of death concurred with the pathological cause in 53% of our cases. C1 NCI,BETHESDA,MD 20892. HOWARD UNIV,COLL MED,DEPT MED,WASHINGTON,DC 20001. HOWARD UNIV,COLL MED,DEPT PATHOL,WASHINGTON,DC 20001. NR 22 TC 27 Z9 28 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PD FEB PY 1992 VL 5 IS 2 BP 132 EP 136 PG 5 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA GZ902 UT WOS:A1992GZ90200005 PM 1732504 ER PT J AU BRODINE, SK OLDFIELD, EC CORWIN, AL THOMAS, RJ RYAN, AB HOLMBERG, J MOLGAARD, CA GOLBECK, AL RYDEN, LA BENENSON, AS ROBERTS, CR BLATTNER, WA AF BRODINE, SK OLDFIELD, EC CORWIN, AL THOMAS, RJ RYAN, AB HOLMBERG, J MOLGAARD, CA GOLBECK, AL RYDEN, LA BENENSON, AS ROBERTS, CR BLATTNER, WA TI HTLV-I AMONG UNITED-STATES MARINES STATIONED IN A HYPERENDEMIC AREA - EVIDENCE FOR FEMALE-TO-MALE SEXUAL TRANSMISSION SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE HUMAN T-CELL LEUKEMIA LYMPHOMA VIRUS; SEXUALLY TRANSMITTED DISEASES ID CELL LEUKEMIA-VIRUS; TROPICAL SPASTIC PARAPARESIS; LYMPHOTROPIC VIRUS; DRUG-ABUSERS; BLOOD-DONORS; ANTIBODIES; INFECTION; LYMPHOMA; SEROPREVALENCE; RETROVIRUS AB Among 5,255 active duty United States Marines on permanent tour in Okinawa, Japan, screened for human T-cell leukemia/lymphoma virus type I (HTLV-I) seropositivity, 3 (0.06%) were confirmed by Western blot analysis to have core and envelope reactivity. All three seropositive individuals have a history of prolonged sexual contact with Okinawan women, and two of the three individuals are married to seropositive Okinawan wives. Two gave a prior history of gonorrhea, while all three were negative for syphilis (MHA-TP) and hepatitis B. No other risk factors associated with HTLV-I seropositivity in the United States were identified. A banked sample from one individual, obtained 8 months after initial sexual relations with his HTLV-I-seropositive Okinawan spouse and 20 months before being retested in the survey, showed a pattern suggesting seroconversion. Although based on small numbers, these data suggest that female-to-male transmission of HTLV-I occurs in the absence of other cofactors, e.g., ulcerative genital lesions. C1 NAVAL HOSP,US PACIFIC COMMAND JOINT BLOOD PROGRAM OFF,OKINAWA,JAPAN. USN HOSP,DEPT CLIN INVEST,SAN DIEGO,CA 92134. USN,MED RES & DEV COMMAND,BETHESDA,MD 20814. USN,ENVIRONM PREVENT MED UNIT 6,HONOLULU,HI. MARINE DIV 3,OKINAWA,JAPAN. SAN DIEGO STATE UNIV,GRAD SCH PUBL HLTH,SAN DIEGO,CA 92182. SAN DIEGO STATE UNIV,DEPT MATH SCI,SAN DIEGO,CA 92182. WALTER REED ARMY MED CTR,DIV RETROVIROL,WASHINGTON,DC 20307. NCI,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892. RP BRODINE, SK (reprint author), USN HOSP,DEPT INTERNAL MED,DIV INFECT DIS,SAN DIEGO,CA 92134, USA. NR 34 TC 10 Z9 10 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PD FEB PY 1992 VL 5 IS 2 BP 158 EP 162 PG 5 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA GZ902 UT WOS:A1992GZ90200010 PM 1732508 ER PT J AU ABRAMCZUK, JW PEZEN, DS LEONARD, JM MONELLTORRENS, E BELCHER, JH MARTIN, MA NOTKINS, AL AF ABRAMCZUK, JW PEZEN, DS LEONARD, JM MONELLTORRENS, E BELCHER, JH MARTIN, MA NOTKINS, AL TI TRANSGENIC MICE CARRYING INTACT HIV PROVIRUS - BIOLOGICAL EFFECTS AND ORGANIZATION OF A TRANSGENE SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE HIV; TRANSGENIC MICE; PROVIRUS; RESTRICTION ENDONUCLEASES ID GENE; EXPRESSION; VIRUS; DNA; CELLS; RETROVIRUS; CLONE AB Twelve transgenic founder animals retaining intact copies of the infectious molecular clone of human immunodeficiency virus (HIV)-1 were obtained. All the founders appeared healthy during a 9- to 12-month observation period. However, transgenic offspring of one of the founders (female # 13), died within the 1st month of life while manifesting several symptoms characteristic of human AIDS. To discover why only one transgenic lineage was affected and why the founder animal in the affected lineage remained healthy while all of her transgenic offspring were diseased, we compared the organization of the transgene in the transgenic lineages. Restriction enzyme analysis showed that the founder no. 13 was a mosaic carrying in each transgenic cell four tandemly arranged copies of the infectious molecular clone. All the units of the tandem repeat appeared to be correctly preserved with the exception of the 3'-most copy, which terminated near the start of human sequences that flank the 3' long terminal repeat (LTR). The unaffected founders and their transgenic litter usually carried a high number of copies of the provirus. The 5' terminus of the transgene in the unaffected animals appeared to be deleted or rearranged. None of the 12 transgenic founders carried a single copy of integrated provirus. We conclude that infectious molecular clone of HIV-1 can be expressed in transgenic mice, and that the mode of proviral integration similar to that seen during the retroviral infectious cycle (i.e., a single-copy provirus) may be incompatible with the postnatal survival. It also appears that preservation of the 5' human DNA flank and/or 5' LTR, and their association with the murine sequences are required for the proviral expression. In addition, we suggest that the systemic symptoms in the affected animals of lineage no. 13 are induced by the factors released from the diseased skin. C1 NIDR,ORAL MED LAB,BETHESDA,MD 20892. HOWARD HUGHES MED INST,BETHESDA,MD. NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. NR 16 TC 12 Z9 12 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PD FEB PY 1992 VL 5 IS 2 BP 196 EP 203 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA GZ902 UT WOS:A1992GZ90200015 PM 1732512 ER PT J AU CORYELL, W WINOKUR, G KELLER, M SCHEFTNER, W ENDICOTT, J AF CORYELL, W WINOKUR, G KELLER, M SCHEFTNER, W ENDICOTT, J TI ALCOHOLISM AND PRIMARY MAJOR DEPRESSION - A FAMILY STUDY APPROACH TO COEXISTING DISORDERS SO JOURNAL OF AFFECTIVE DISORDERS LA English DT Article DE ALCOHOLISM; MAJOR DEPRESSION; FAMILY STUDY ID SECONDARY AFFECTIVE-DISORDER; CRITERIA; UNIPOLAR AB Alcoholism and major depression appear together at much higher than chance rates, but reasons for this are obscure. We used the direct diagnostic assessment of 177 probands with primary, unipolar depression and 619 of their first degree relatives to explore the significance of concomitant alcoholism. The male relatives of alcoholic probands of both sexes had substantially higher rates of alcoholism than did the male relatives of non-alcoholic probands. Among female probands, but not among male probands, alcoholism was associated with markedly higher familial rates of primary depression, particularly among female relatives. These data contained no evidence that comorbitity itself was familial. The appearance of alcoholism in depressed women may indicate depression spectrum disease, a disorder which manifests as depression in women and alcoholism in men. In contrast, men with both primary depression and alcoholism may be exhibiting two distinct illnesses. C1 UNIV IOWA,COLL MED,DEPT PSYCHIAT,500 NEWTON RD,MEB,IOWA CITY,IA 52242. NIMH,COLLABORAT PROGRAM PSYCHOBIOL DEPRESS CLIN STUDIES,BETHESDA,MD 20892. FU NIMH NIH HHS [R01 MH025478] NR 25 TC 45 Z9 45 U1 5 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-0327 J9 J AFFECT DISORDERS JI J. Affect. Disord. PD FEB PY 1992 VL 24 IS 2 BP 93 EP 99 DI 10.1016/0165-0327(92)90023-Y PG 7 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA HE029 UT WOS:A1992HE02900005 PM 1541771 ER PT J AU MULLOL, J RAPHAEL, GD LUNDGREN, JD BARANIUK, JN MERIDA, M SHELHAMER, JH KALINER, MA AF MULLOL, J RAPHAEL, GD LUNDGREN, JD BARANIUK, JN MERIDA, M SHELHAMER, JH KALINER, MA TI COMPARISON OF HUMAN NASAL MUCOSAL SECRETION INVIVO AND INVITRO SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE RESPIRATORY GLYCOCONJUGATES; LACTOFERRIN; EXPLANT CULTURE; ALBUMIN; METHACHOLINE; ATROPINE; HISTAMINE; ALPHA-AGONISTS; BETA-AGONISTS ID PATHO-PHYSIOLOGY; MUCOUS GLYCOPROTEINS; HUMAN AIRWAYS; RHINITIS; RELEASE; HISTAMINE; PROTEIN; LOCALIZATION; LACTOFERRIN; STIMULATION AB The secretion of proteins from the human nasal mucosa induced by histamine, alpha-adrenergic, beta-adrenergic, and cholinergic agonists was studied in vivo and in vitro. Glandular secretion of lactoferrin, lysozyme (in vivo only), and respiratory glycoconjugates (RGCs) was measured. Vascular permeability was determined in vivo by albumin secretion in relationship to the other proteins. Muscarinic stimulation by methacholine induced significant glandular secretion (lactoferrin, lysozyme and/or RCGs) both in vivo and in vitro, confirming that muscarinic receptors are stimulated directly. Histamine induced predominantly vascular permeability in vivo but caused some glandular secretion as well. However, in vitro, histamine had no effect on glandular secretion, suggesting that histamine acts predominantly on the nasal vascular bed and only affects glandular secretion through reflex actions. Phenylephrine, an alpha-adrenergic agonist, selectively stimulated lysozyme release in vivo, and both RGCs and lactoferrin release in vitro. Thus, alpha-adrenergic stimulation has some direct, albeit minimal, capacity to stimulate mucosal glands. Beta-Adrenergic agonists had no effect on glandular secretion or vascular permeability either in vivo or in vitro. Therefore, glandular secretion is directly stimulated by alpha-adrenergic and cholinergic agonists, but not by beta-adrenergic agonists. The stimulation of glandular secretion by histamine is indirect and mediated through the action of neural reflexes. C1 NIAID,CLIN INVEST LAB,ALLERG DIS SECT,BLDG 10,RM 11-C-205,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT CRIT CARE MED,BETHESDA,MD 20892. OI Lundgren, Jens/0000-0001-8901-7850 NR 30 TC 35 Z9 37 U1 0 U2 2 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 1992 VL 89 IS 2 BP 584 EP 592 DI 10.1016/0091-6749(92)90326-W PG 9 WC Allergy; Immunology SC Allergy; Immunology GA HD753 UT WOS:A1992HD75300011 PM 1740587 ER PT J AU KAULBACH, HC IGARASHI, Y MULLOL, J WHITE, MV KALINER, MA AF KAULBACH, HC IGARASHI, Y MULLOL, J WHITE, MV KALINER, MA TI EFFECTS OF NEDOCROMIL SODIUM ON ALLERGEN-INDUCED RHINITIS IN HUMANS SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE NEDOCROMIL SODIUM; NASAL SECRETIONS; ALLERGEN CHALLENGE ID 1-PERCENT NASAL SOLUTION; PATHO-PHYSIOLOGY; CROMOLYN SODIUM; SECRETIONS; HISTAMINE; PLACEBO; RADIOIMMUNOASSAY; PROVOCATION; PROTEIN AB Sixteen patients with allergic rhinitis were recruited into a double-blind crossover protocol studying the immediate effect of nedocromil sodium (NS) on the pattern of nasal symptoms and secretions after allergen challenge. After pretreatment with placebo or NS, allergen challenge resulted in pruritus, rhinorrhea, nasal congestion, and/or sneezing within 10 minutes in 12 of 16 subjects. Prostaglandin D2 (PGD2), a marker of mast cell degranulation, increased proportionately with symptom scores, remaining above the 95% confidence interval for 120 minutes after both pretreatments. No difference in PGD2 between the NS-treatment and placebo-treatment days was observed. Protein markers extravasated through the vasculature (albumin and IgG) or secreted by mucosal glands (lactoferrin) were assayed. Total protein, albumin, IgG, and lactoferrin all remained > 95% confidence interval for 100 minutes after allergen challenge in the placebo-pretreated group and 120 minutes in the NS-pretreated group. Although there appeared to be a trend for lower secretion of PGD2, albumin, and IgG in the NS-treated group, the overall differences did not achieve statistical significance. This protocol revealed that two topical 130-mu-l doses of a 1% solution of NS failed to significantly reduce allergen-induced symptoms, PGD2 generation, or secretion of albumin, IgG, or lactoferrin when NS was compared with placebo. The anti-inflammatory and mast cell-stabilizing effects of NS may require more prolonged pretreatment before provocation to be effective. C1 NIAID,CLIN INVEST LAB,ALLERG DIS SECT,BLDG 10,ROOM 11C205,BETHESDA,MD 20892. NR 26 TC 10 Z9 10 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 1992 VL 89 IS 2 BP 599 EP 610 DI 10.1016/0091-6749(92)90328-Y PG 12 WC Allergy; Immunology SC Allergy; Immunology GA HD753 UT WOS:A1992HD75300013 PM 1311008 ER PT J AU SAMUDZI, CT ROSENBERG, JM AF SAMUDZI, CT ROSENBERG, JM TI DETECTION OF PATTERSON GHOST PEAKS AND SHAPE DISTORTIONS DUE TO SAMPLING IN PATTERSON FUNCTIONS FROM INCOMPLETE DATA SETS SO JOURNAL OF APPLIED CRYSTALLOGRAPHY LA English DT Article AB A procedure for evaluating peaks in the difference Patterson maps calculated from a complete native data set and partial (incomplete) potential isomorphous heavy-atom-derivative data sets is described. A sampling function is defined such that it is unity where data are available and zero elsewhere. It is the Patterson of this sampling function whose characteristics are useful. This function will not reconstruct unavailable information, but can reveal the presence of distorted and/or 'false' peaks introduced by the sampling technique used in collecting partial data. This procedure is useful in screening potential heavy-atom derivatives using a minimum number of crystals. C1 UNIV PITTSBURGH,DEPT BIOL SCI,PITTSBURGH,PA 15260. RP SAMUDZI, CT (reprint author), NCI,FCRDC,ABL BASIC RES PROGRAM,POB B,FREDERICK,MD 21702, USA. NR 5 TC 3 Z9 3 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0021-8898 J9 J APPL CRYSTALLOGR JI J. Appl. Crystallogr. PD FEB 1 PY 1992 VL 25 BP 65 EP 68 DI 10.1107/S0021889891010373 PN 1 PG 4 WC Chemistry, Multidisciplinary; Crystallography SC Chemistry; Crystallography GA HD733 UT WOS:A1992HD73300010 ER PT J AU NIOKA, S ARGOV, Z DOBSON, GP FORSTER, RE SUBRAMANIAN, HV VEECH, RL CHANCE, B AF NIOKA, S ARGOV, Z DOBSON, GP FORSTER, RE SUBRAMANIAN, HV VEECH, RL CHANCE, B TI SUBSTRATE REGULATION OF MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION IN HYPERCAPNIC RABBIT MUSCLE SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE ISOMETRIC TENSION-TIME INTEGRAL; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; ADENOSINE DIPHOSPHATE; INORGANIC PHOSPHATE; MAXIMAL REACTION VELOCITY; ACIDOSIS ID NUCLEAR MAGNETIC-RESONANCE; SARCOPLASMIC-RETICULUM; ENERGY-METABOLISM; MUSCULAR FATIGUE; SKELETAL-MUSCLES; CARBON-DIOXIDE; TWITCH MUSCLE; DOG BRAIN; RAT; PH AB Endurance muscle performance is highly dependent on ATP production from mitochondrial oxidative phosphorylation. To study the role of the mitochondrial oxidative enzymes in muscle fatigue, we analyzed the relationship between the concentrations of substrates associated with ATP synthesis and the muscle performance of electrically stimulated rabbit muscle under CO2-induced acidosis. Two different conditions of pacing-induced muscle performance were produced in the gastrocnemius and soleus muscle groups in anesthetized rabbits by stimulating the sciatic nerve submaximally at two frequencies. Phosphorus nuclear magnetic resonance was used to measure ATP, phosphocreatine, and P(i) and to provide data for a calculation of intracellular pH and free ADP. To induce acidosis, the animal was ventilated with 20% CO2. The administration of CO2 effectively reduced the intracellular pH from 6.9 to 6.7 and reduced the isometric tension-time integral (TTI) to below half the value measured in normocapnia at the low pacing frequency. A twofold increase in the pacing frequency resulted in a doubling of the TTI in normocapnia and a tripling of TTI in hypercapnia. The increases in TTI corresponded with increases in free ADP and P(i) concentrations. Under the various conditions, all free ADP values were near the in vitro Michaelis-Menten constant (K(m)) of ADP. The Michaelis-Menten relationship of the oxidative phosphorylative enzymes was applied to the change in substrate concentrations with respect to TTI. From this relationship we observed that the in vivo K(m) of free ADP was 26-mu-M, which is close to the in vitro K(m), and that K(m) and maximal reaction velocity did not change under hypercapnia and increased pacing frequency. From our observations, we conclude that free ADP regulates ATP synthesis in normocapnia and hypercapnia and that hypercapnia-induced acidosis does not inhibit ATP synthesis through the substrate regulation mechanism. C1 UNIV PENN,DEPT PHYSIOL,PHILADELPHIA,PA 19104. HADASSAH UNIV HOSP,KIRYAT HADASSAH,JERUSALEM,ISRAEL. NIAAA,METAB LAB,ROCKVILLE,MD 20850. RP NIOKA, S (reprint author), UNIV PENN,SCH MED,DEPT BIOCHEM BIOPHYS,PHILADELPHIA,PA 19104, USA. FU NHLBI NIH HHS [HL-19737, HL-31934] NR 52 TC 41 Z9 41 U1 0 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD FEB PY 1992 VL 72 IS 2 BP 521 EP 528 PG 8 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA HE268 UT WOS:A1992HE26800018 PM 1559927 ER PT J AU SULLIVAN, SL WARD, DF GOTTESMAN, ME AF SULLIVAN, SL WARD, DF GOTTESMAN, ME TI EFFECT OF ESCHERICHIA-COLI-NUSG FUNCTION ON LAMBDA N-MEDIATED TRANSCRIPTION ANTITERMINATION SO JOURNAL OF BACTERIOLOGY LA English DT Article ID BACTERIOPHAGE-LAMBDA; RNA-POLYMERASE; GENE PROTEIN; ANTI-TERMINATION; PHAGE-LAMBDA; HOST MUTANTS; OPERON; EXPRESSION; INITIATION; PROMOTERS AB The Escherichia coli Nus factors act in conjunction with the bacteriophage lambda-N protein to suppress transcription termination on the lambda-chromosome. NusA binds both N and RNA polymerase and may also interact with other Nus factors. To search for additional components of the N antitermination system, we isolated host revertants that restored N activity in nusA1 mutants. One revertant, nusG4, was mapped to the rif region of the E. coli chromosome and shown to represent a point mutation near the 3' end of the nusG gene. The nusG4 mutation also suppressed nusE71 but not nusA(Sal), nusB5, nusC60 (rpoB60), or nusD026 (rho026). However, nusG+ expressed from a multicopy plasmid suppressed nusD026 and related rho mutants for both lambda and phage T4 growth. These results suggest that NusG may act as a component of the N antitermination complex. In addition, the data imply a role for NusG in Rho-dependent termination. C1 COLUMBIA UNIV COLL PHYS & SURG,INST CANC RES,701 W 168TH ST,NEW YORK,NY 10032. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. FU NIGMS NIH HHS [GM37219] NR 41 TC 57 Z9 57 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0021-9193 J9 J BACTERIOL JI J. Bacteriol. PD FEB PY 1992 VL 174 IS 4 BP 1339 EP 1344 PG 6 WC Microbiology SC Microbiology GA HD467 UT WOS:A1992HD46700031 PM 1531224 ER PT J AU FISCHER, D BACHAR, O NUSSINOV, R WOLFSON, H AF FISCHER, D BACHAR, O NUSSINOV, R WOLFSON, H TI AN EFFICIENT AUTOMATED COMPUTER VISION BASED TECHNIQUE FOR DETECTION OF 3-DIMENSIONAL STRUCTURAL MOTIFS IN PROTEINS SO JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS LA English DT Article ID GENERAL METHOD; BETA-SHEETS; EVOLUTION; IDENTIFICATION; RECOGNITION; TAXONOMY; SEQUENCE AB As the number of available three dimensional coordinates of proteins increases, it is now recognized that proteins from different families and topologies are constructed from independent motifs. Detection of specific structural motifs within proteins aids in understanding their role and the mechanism of their operation. To aid in identification and use of these motifs it has become necessary to develop efficient methods for systematic scanning of structural databases. To date, methods of structural protein comparison suffer from at least one of the following limitations: (1) are not fully automated (require human intervention), (2) are limited to relatively similar structures, (3) are constrained to linear alignments of the structures, (4) are sensitive to insertions, deletions or gaps in the sequences or (5) are very time consuming. We present a method to overcome the above limitations. The method discovers and ranks every piece of structural similarity between the structures compared, thus allowing the simultaneous detection of real 3-D motifs in different domains, between domains, in active sites, surfaces etc. The method uses the Geometric Hashing Paradigm which is an efficient technique originally developed for Computer Vision. The algorithm exploits the geometrical constraints of rigid objects, it is especially geared towards recognition of partial structures in rigid objects belonging to large data bases and is straightforwardly parallelizable. Computer Vision techniques are for the first time applied to molecular structure comparison, resulting in an efficient, fully automated tool. The method has been tested in a number of cases, including comparisons of the haemoglobins, immunoglobulins, serine proteinases, calcium binding proteins, DNA binding proteins and others. In all examples our results were equivalent to the published results from previous methods and in some cases additional structural information was obtained by our method. C1 TEL AVIV UNIV,FAC MED,SACKLER INST MOLEC MED,IL-69978 TEL AVIV,ISRAEL. TEL AVIV UNIV,SCH MATH SCI,DEPT COMP SCI,IL-69978 TEL AVIV,ISRAEL. NCI,FREDERICK CANC RES FACIL,PRI DYNACORP,MATH BIOL LAB,FREDERICK,MD 21701. NYU,COURANT INST MATH SCI,ROBOT RES LAB,NEW YORK,NY 10012. RI Wolfson, Haim/A-1837-2011 FU NCI NIH HHS [1-CO-74102] NR 29 TC 65 Z9 66 U1 0 U2 0 PU ADENINE PRESS INC PI GUILDERLAND PA PO BOX 355/340, GUILDERLAND, NY 12084 SN 0739-1102 J9 J BIOMOL STRUCT DYN JI J. Biomol. Struct. Dyn. PD FEB PY 1992 VL 9 IS 4 BP 769 EP 789 PG 21 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA HH959 UT WOS:A1992HH95900012 PM 1616630 ER PT J AU OLIVER, C SAHARA, N KITANI, S ROBBINS, AR MERTZ, LM SIRAGANIAN, RP AF OLIVER, C SAHARA, N KITANI, S ROBBINS, AR MERTZ, LM SIRAGANIAN, RP TI BINDING OF MONOCLONAL-ANTIBODY AA4 TO GANGLIOSIDES ON RAT BASOPHILIC LEUKEMIA-CELLS PRODUCES CHANGES SIMILAR TO THOSE SEEN WITH FC-EPSILON RECEPTOR ACTIVATION SO JOURNAL OF CELL BIOLOGY LA English DT Article ID PROTEIN KINASE-C; MICROTUBULE-ASSOCIATED PROTEIN-2; GROWTH-FACTOR RECEPTOR; HISTAMINE-RELEASE; CROSS-LINKING; 2H3 CELLS; TYROSINE PHOSPHORYLATION; POSSIBLE INVOLVEMENT; SIGNAL TRANSDUCTION; MEDIATED MODULATION AB The mAb AA4 binds to novel derivatives of the ganglioside G(D1b) rat basophilic leukemia (RBL-2H3) cells. Some of the gangliosides are located close to the high affinity IgE receptor (Fc-epsilon-RI), and binding of mAb AA4 inhibits Fc-epsilon-RI-mediated histamine release. In the present study, mAb AA4 was found to bind exclusively to mast cells in all rat tissues examined. In vitro, within 1 min of mAb AA4 binding, the cells underwent striking morphologic changes. They lost their normal spindle shaped appearance, increased their ruffling, and spread over the surface of the culture dish. These changes were accompanied by a redistribution of the cytoskeletal elements, actin, tubulin, and vimentin, but only the actin was associated with the membrane ruffles. Binding of mAb AA4 also induces a rise in intracellular calcium, stimulates phosphatidyl inositol breakdown, and activates PKC. However, the extent of these changes was less than that observed when the cells were stimulated with antigen or antibody directed against the Fc-epsilon-RI. None of these changes associated with mAb AA4 binding were seen when the cells were exposed to nonspecific IgG, IgE, or four other anti-cell surface antibodies, nor were the changes induced by binding mAb AA4 at 4-degrees-C or in the absence of extracellular calcium. Although mAb AA4 does not stimulate histamine release, it enhances the effect of the calcium ionophore A23187 mediated release. The morphological and biochemical effects produced by mAb AA4 are similar to those seen following activation of the cell through the IgE receptor. Therefore, the surface gangliosides which bind mAb AA4 may function in modulating secretory events. C1 NIDR, CLIN INVEST & PATIENT CARE BRANCH, BETHESDA, MD 20892 USA. NIDDKD, BIOCHEM & METAB LAB, BETHESDA, MD USA. RP OLIVER, C (reprint author), NIDR, IMMUNOL LAB, BETHESDA, MD 20892 USA. NR 55 TC 44 Z9 45 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA SN 0021-9525 EI 1540-8140 J9 J CELL BIOL JI J. Cell Biol. PD FEB PY 1992 VL 116 IS 3 BP 635 EP 646 DI 10.1083/jcb.116.3.635 PG 12 WC Cell Biology SC Cell Biology GA HA992 UT WOS:A1992HA99200007 PM 1370498 ER PT J AU JAKOWLEW, SB CUBERT, J DANIELPOUR, D SPORN, MB ROBERTS, AB AF JAKOWLEW, SB CUBERT, J DANIELPOUR, D SPORN, MB ROBERTS, AB TI DIFFERENTIAL REGULATION OF THE EXPRESSION OF TRANSFORMING GROWTH-FACTOR-BETA MESSENGER-RNAS BY GROWTH-FACTORS AND RETINOIC ACID IN CHICKEN-EMBRYO CHONDROCYTES, MYOCYTES, AND FIBROBLASTS SO JOURNAL OF CELLULAR PHYSIOLOGY LA English DT Article ID FACTOR-BETA-1 GENE; MOUSE KERATINOCYTES; MOLECULAR-CLONING; RIBONUCLEIC-ACID; PROMOTER; SEQUENCES; CELLS; LIMB; PURIFICATION; MODULATION AB Transforming growth factor-beta (TGF-beta) autoregulates its expression in several mammalian cell types. We now report that addition of TGF-beta-s 1, 2, and 3 to primary chicken embryo cells differentially affects expression of the messenger RNAs for the different TGF-beta isoforms depending on the cell type. In cultured sternal chondrocytes, addition of TGF-beta-s 1, 2, or 3 results in an increase in the steady-state levels of the messenger RNAs for TGF-beta-s 2 and 3, but does not change expression of TGF-beta-4 mRNA. In contrast, in cultured cardiac myocytes, addition of TGF-beta-s 1, 2, or 3 results in an increase in expression of TGF-beta-s 3 and 4 mRNAs, but does not change expression of TGF-beta-2 mRNA. Moreover, expression of TGF-beta-s 2, 3, and 4 mRNAs is not affected by addition of any of the TGF-beta-s to fibroblasts. Addition of platelet-derived growth factor (PDGF), epidermal growth factor (EGF), or interleukin-1 (IL-1) to these chicken cells also has differential effects on expression of the different TGF-beta mRNAs depending on the cell type. Retinoic acid also has contrasting effects on chondrocytes and myocytes either increasing or decreasing, respectively, expression of TGF-beta-s 2 and 3 mRNAs and TGF-beta-2 protein. Our results indicate a complex pattern of regulation of the different TGF-beta genes by themselves as well as by PDGF, EGF, IL-1, dexamethasone, TPA, and retinoic acid in chicken embryo cells. RP JAKOWLEW, SB (reprint author), NCI, CHEMOPREVENT LAB, BETHESDA, MD 20892 USA. NR 42 TC 59 Z9 59 U1 0 U2 0 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0021-9541 J9 J CELL PHYSIOL JI J. Cell. Physiol. PD FEB PY 1992 VL 150 IS 2 BP 377 EP 385 DI 10.1002/jcp.1041500222 PG 9 WC Cell Biology; Physiology SC Cell Biology; Physiology GA HD553 UT WOS:A1992HD55300021 PM 1734039 ER PT J AU PERINI, GI DEVINSKY, O HAUSER, P GALLUCCI, WT THEODORE, WH CHROUSOS, GP GOLD, PW KLING, MA AF PERINI, GI DEVINSKY, O HAUSER, P GALLUCCI, WT THEODORE, WH CHROUSOS, GP GOLD, PW KLING, MA TI EFFECTS OF CARBAMAZEPINE ON PITUITARY-ADRENAL-FUNCTION IN HEALTHY-VOLUNTEERS SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID CORTICOTROPIN-RELEASING HORMONE; BENZODIAZEPINE RECEPTORS; BIOCHEMICAL MANIFESTATIONS; AFFECTIVE-ILLNESS; VASOPRESSIN; DEPRESSION; RESPONSES; STRESS; PLASMA; NEUROBIOLOGY AB Carbamazepine (CBZ) is a widely used therapeutic agent in seizure, pain, and mood disorders. Although CBZ has been shown to inhibit hypothalamic CRH secretion in vitro, limited data suggest that systemic CBZ induces pituitary-adrenal activation. Few data are available to reconcile these effects or clarify their mechanism(s), particularly in healthy human subjects. We report here a study of basal ACTH and cortisol secretion and their responses to ovine CRH administration in nine healthy volunteers, studied both during repeated (2-3 weeks) administration of CBZ and while medication free. CBZ significantly increased mean 24-h urinary free cortisol (mean +/- SE, 197 +/- 17 vs. 137 +/- 24 nmol/day; P < 0.02) and evening basal total plasma cortisol (113 +/- 17 vs. 83 +/- 14 nmol/L; P < 0.05) as well as cortisol-binding globulin-binding capacity (497 +/- 36 vs. 433 +/- 28 nmol/L; P < 0.01). Despite the CBZ-induced hypercortisolism, plasma ACTH responses to CRH during CBZ treatment remained robust, rather than being suppressed by basal hypercortisolism. In fact, during CBZ treatment, we noted a positive correlation between the increase in basal plasma cortisol and the increase in the plasma ACTH response to CRH (r = 0.65; P < 0.05). We also observed a reduction in cortisol-binding globulin-binding capacity after CRH administration (315 +/- 25 vs. 433 +/- 28 nmol/L; P < 0.001), which was accentuated by CBZ treatment (342 +/- 19 vs. 497 +/- 36 nmol/L; P < 0.001; magnitude of fall, -155 +/- 22 nmol/L on CBZ vs. -118 +/- 11 nmol/L off CBZ; P < 0.05). We conclude that CBZ increases plasma cortisol secretion in healthy volunteers independent of its effect on plasma cortisol-binding capacity. This pituitary-adrenal activation seems to reflect a pituitary, rather than a hypothalamic, effect of CBZ. Hence, despite CBZ-induced hypercortisolism, the ACTH response to CRH remained robust in direct proportion to the CBZ-induced rise in basal plasma cortisol. Thus, we propose that the increased cortisol secretion observed during CBZ treatment reflects a relative inefficacy of glucocorticoid negative feedback at the pituitary. This pituitary-driven increase in cortisol secretion combined with the expected reduction in centrally directed CRH secretion could contribute to the anticonvulsant properties of CBZ. C1 NIMH, CLIN NEUROENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. NIMH, BIOL PSYCHIAT BRANCH, BETHESDA, MD 20892 USA. NINCDS, MED NEUROL BRANCH, BETHESDA, MD 20892 USA. NICHHD, DEV ENDOCRINOL BRANCH, BETHESDA, MD 20892 USA. RI Kling, Mitchel/F-4152-2010; OI Kling, Mitchel/0000-0002-2232-1409; PERINI, GIULIA/0000-0003-3261-1984 NR 35 TC 23 Z9 23 U1 1 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD FEB PY 1992 VL 74 IS 2 BP 406 EP 412 DI 10.1210/jc.74.2.406 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HE384 UT WOS:A1992HE38400029 PM 1309836 ER PT J AU HERNANDEZ, ER HURWITZ, A VERA, A PELLICER, A ADASHI, EY LEROITH, D ROBERTS, CT AF HERNANDEZ, ER HURWITZ, A VERA, A PELLICER, A ADASHI, EY LEROITH, D ROBERTS, CT TI EXPRESSION OF THE GENES ENCODING THE INSULIN-LIKE GROWTH-FACTORS AND THEIR RECEPTORS IN THE HUMAN OVARY SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID HUMAN GRANULOSA-CELLS; MESSENGER RIBONUCLEIC-ACID; FACTOR-BINDING PROTEIN; I IGF-I; HORMONAL-REGULATION; LUTEAL CELLS; RAT; RNA; PATHOGENESIS AB Insulin-like growth factor-I (IGF-I) and IGF-II have been proposed as potential regulators of ovarian function. To gain further insight as to the possible role(s) of the IGFs in human ovarian physiology, we have characterized the expression of the genes encoding the IGFs and their corresponding receptors in the human ovary using solution hybridization/RNase protection assays. IGF-I gene expression was evident in liver, placenta, and whole premenopausal ovary, but not in luteinized granulosa cells. Use of 3' - and 5' -specific antisense RNA probes revealed the presence of IGF-I mRNAs encoding both the Ea and Eb forms of the E-peptide as well as potential 5' -untranslated region splicing variants in liver, placenta, and whole menopausal ovary. Immunohistochemical studies localized the IGF-I peptide to the thecal-interstitial compartment. IGF-II mRNA transcribed from the fetal or fetal-neonatal IGF-II promoter was found in whole premenopausal ovary, luteinized granulosa cells, and placenta. Insulin and type I and type II IGF receptor mRNAs were detected in all tissues examined. Two protected probe fragments were seen with the type I IGF receptor probe in each case, suggesting the possibility of alternate splicing. These studies provide further evidence for a role of these growth factors in human ovarian function. C1 HOSP CLIN, DEPT OBSTET GYNECOL, E-46100 VALENCIA, SPAIN. NIDDKD, DIABET BRANCH, MOLEC & CELLULAR PHYSIOL, BETHESDA, MD 20892 USA. UNIV MARYLAND, SCH MED, DEPT OBSTET GYNECOL & PHYSIOL, DIV REPROD ENDOCRINOL, BALTIMORE, MD 21201 USA. RI Manas Carbonero, Patricia/C-1949-2015; OI Manas Carbonero, Patricia/0000-0002-5338-0612; Roberts, Charles/0000-0003-1756-5772 FU NICHD NIH HHS [1ROI-HD-19998, HD-00697] NR 38 TC 185 Z9 188 U1 0 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD FEB PY 1992 VL 74 IS 2 BP 419 EP 425 DI 10.1210/jc.74.2.419 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HE384 UT WOS:A1992HE38400031 PM 1309838 ER PT J AU URIARTE, MM BARON, J GARCIA, HB BARNES, KM LORIAUX, DL CUTLER, GB AF URIARTE, MM BARON, J GARCIA, HB BARNES, KM LORIAUX, DL CUTLER, GB TI THE EFFECT OF PUBERTAL DELAY ON ADULT HEIGHT IN MEN WITH ISOLATED HYPOGONADOTROPIC HYPOGONADISM SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID ISOLATED GONADOTROPIN-DEFICIENCY; ADOLESCENT GROWTH SPURT; FINAL HEIGHT; BOYS; GIRLS; PATTERN; ONSET; AGE AB To determine whether delay of puberty alters adult height, we retrospectively evaluated the adult height of 41 patients who met rigorous criteria for the diagnosis of isolated hypogonadotropic hypogonadism. The adult height of these patients was compared with the mean height of normal American men at age 18 in the 1979 National Center for Health Statistics survey and with the mean adult height of 50 male normal volunteers who had been studied on the same wards as the patients with hypogonadotropic hypogonadism. The mean adult height of the men with isolated hypogonadotropic hypogonadism was 179.7 cm, which exceeded the height of the 50 control subjects and the normal American men (both 176.8 cm) by 2.9 cm (P < 0.05 and P < 0.006, respectively). The mean age at treatment to induce puberty was 20.0 yr, which corresponded to a mean delay in the onset of puberty of more than 8 yr relative to normal males. The final height of the men with hypogonadotropic hypogonadism correlated significantly with the duration of pubertal delay (r = 0.32, P = 0.04). Most of the enhanced mean adult height of the patients with isolated hypogonadotropic hypogonadism was attributable to those patients in whom treatment to induce puberty had been delayed to age 18 or greater. The mean adult height of these patients was 182.4 cm, or 5.6 cm greater than the mean height of the 50 controls or of normal American men (P < 0.001). The mean adult height of patients whose treatment began between 10 and 17 yr of age was 175.0 cm, which did not differ significantly from that of normal men. We conclude that prolonged delay of puberty (6 or more yr) in men with isolated hypogonadotropic hypogonadism is associated with a modest increase (approximately 5 cm) of adult height. C1 NICHHD, DEV ENDOCRINOL BRANCH, BLDG 10, ROOM 10-N-262, BETHESDA, MD 20892 USA. NR 25 TC 25 Z9 25 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD FEB PY 1992 VL 74 IS 2 BP 436 EP 440 DI 10.1210/jc.74.2.436 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HE384 UT WOS:A1992HE38400033 PM 1449545 ER PT J AU STEINFELDER, HJ RADOVICK, S MROCZYNSKI, MA HAUSER, P MCCLASKEY, JH WEINTRAUB, BD WONDISFORD, FE AF STEINFELDER, HJ RADOVICK, S MROCZYNSKI, MA HAUSER, P MCCLASKEY, JH WEINTRAUB, BD WONDISFORD, FE TI ROLE OF A PITUITARY-SPECIFIC TRANSCRIPTION FACTOR (PIT-1/GHF-1) OR A CLOSELY RELATED PROTEIN IN CAMP REGULATION OF HUMAN THYROTROPIN-BETA SUBUNIT GENE-EXPRESSION SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE CAMP; PIT-1; TSH-B; TRANSCRIPTION FACTOR; PHOSPHORYLATION ID HUMAN CHORIONIC-GONADOTROPIN; 3',5'-CYCLIC ADENOSINE-MONOPHOSPHATE; CELL-SPECIFIC EXPRESSION; RAT GROWTH-HORMONE; CYCLIC-AMP; SOMATOSTATIN GENE; THYROID-HORMONE; RESPONSIVE ELEMENT; PROLACTIN GENE; MESSENGER-RNA AB cAMP regulation of the human thyrotropin-beta (TSH-beta) gene cAMP was studied in two heterologous cell lines, a human embryonal kidney cell line (293) and a rat pituitary cell line (GH3). In 293 cells, human TSH-beta gene expression was not stimulated by the adenylate cyclase activator forskolin or the cAMP analogue 8-bromo-cAMP (8-Br-cAMP). On the other hand, these agents induced human TSH-beta gene expression 4-12-fold in GH3 cells. Deletion analysis demonstrated that the regions from +3 to +8 bp and from -128 to -61 bp were both necessary for cAMP stimulation. The latter region contains three DNA sequences homologous to a pituitary-specific transcription factor, Pit-1/GHF-1, DNA-binding site. Gel-mobility assays demonstrated that a radiolabeled human TSH-beta probe (-128 to -61 bp) formed five specific DNA-protein complexes with mouse thyrotropic tumor (MTT) nuclear extract and two specific complexes with in vitro translated Pit-1/GHF-1. Four of the five MTT complexes and both in vitro Pit-1/GHF-1 complexes were reduced or eliminated by excess of an unlabeled Pit-1/GHF-1 DNA-binding site from the rat growth hormone gene, but not a mutated version of the same DNA fragment, suggesting that Pit-1/GHF-1 or a closely related thyrotroph protein binds to these DNA sequences. In 293 cells, co-transfection of an expression vector containing the Pit-1/GHF-1 cDNA restored cAMP-responsiveness to the human TSH-beta promoter (5.2- and 6.6-fold maximal stimulation by 8-Br-cAMP and forskolin, respectively) but not the herpes virus thymidine kinase promoter (1.2-fold maximal stimulation by either agent). Thus we conclude that the human TSH-beta gene is positively regulated by cAMP in GH3 but not 293 cells. Since the human TSH-beta gene contains at least one high-affinity binding site for Pit-1/GHF-1 in a region necessary for cAMP stimulation and cAMP stimulation could be restored to the human TSH-beta promoter in a previously nonresponsive cell line by the addition of Pit-1/GHF-1, this suggests that Pit-1/GHF-1, or a closely related protein in the thyrotroph, may be a trans-acting factor for cAMP stimulation of the TSH-beta gene. C1 CASE WESTERN RESERVE UNIV,SCH MED,W147,2119 ABINGTON RD,CLEVELAND,OH 44106. UNIV HOSP CLEVELAND,DIV PEDIAT & ADULT ENDOCRINOL,CLEVELAND,OH 44106. NIDDKD,MOLEC CELLULAR & NUTR ENDOCRINOL BRANCH,BETHESDA,MD 20892. FU NIDDK NIH HHS [DK43653-01] NR 39 TC 57 Z9 58 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 1992 VL 89 IS 2 BP 409 EP 419 DI 10.1172/JCI115600 PG 11 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HD270 UT WOS:A1992HD27000009 PM 1310694 ER PT J AU KLEIN, HG LOHSE, P PRITCHARD, PH BOJANOVSKI, D SCHMIDT, H BREWER, HB AF KLEIN, HG LOHSE, P PRITCHARD, PH BOJANOVSKI, D SCHMIDT, H BREWER, HB TI 2 DIFFERENT ALLELIC MUTATIONS IN THE LECITHIN-CHOLESTEROL ACYLTRANSFERASE GENE ASSOCIATED WITH THE FISH EYE SYNDROME - LECITHIN-CHOLESTEROL ACYLTRANSFERASE (THR123-]ILE) AND LECITHIN-CHOLESTEROL ACYLTRANSFERASE (THR347-]MET) SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE CORNEAL OPACITIES; DNA SEQUENCE ANALYSIS; HYPOALPHALIPOPROTEINEMIA; LECITHIN-CHOLESTEROL ACYLTRANSFERASE ACTIVITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM ID HIGH-DENSITY LIPOPROTEINS; MASSIVE CORNEAL OPACITIES; APOLIPOPROTEIN-A-I; HUMAN-PLASMA; SECONDARY STRUCTURE; ACYL TRANSFERASE; NUCLEOTIDE-SEQUENCE; FAMILIAL CONDITION; GENOMIC SEQUENCES; GLOBULAR-PROTEINS AB We have elucidated the genetic defect in a 66-yr-old patient with fish eye syndrome (FES) presenting with severe corneal opacities and hypoalphalipoproteinemia. The patient's plasma concentration of high density lipoprotein (HDL) cholesterol was reduced at 7.7 mg/dl (35.1-65.3 mg/dl in controls) and the HDL cholesteryl ester content was 31% (60-80% in controls); however, total plasma cholesteryl esters were similar to normal (60% of total cholesterol vs. a mean of 66% in controls). The patient's plasma cholesterol esterification rate was slightly reduced at 51 nmol/ml per h (control subjects: 61-106 nmol/ml per h), whereas lecithin-cholesterol acyltransferase (LCAT) activity, assayed using a HDL-like exogenous proteoliposome substrate, was virtually absent (0.9 nmol/ml per h vs. 25.1-27.9 nmol/ml per h in control subjects). DNA sequence analysis of the proband's LCAT gene revealed two separate C to T transitions resulting in the substitution of Thr123 with Ile and Thr347 with Met. The mutation at codon 347 created a new restriction site for the enzyme Nla III. Analysis of the patient's polymerase chain reaction-amplified DNA containing the region of the Thr347 mutation by digestion with Nla III confirmed that the proband is a compound heterozygote for both defects. The patient's daughter, who is asymptomatic despite a 50% reduction of LCAT activity, is heterozygous for the Thr123 --> Ile mutation. Our data indicate that the regions adjacent to Thr123 and Thr347 of LCAT may play an important role in HDL cholesterol esterification, suggesting that these regions may contain a portion of the LCAT binding domain(s) for HDL. C1 UNIV BRITISH COLUMBIA,DEPT PATHOL,LIPOPROT RES GRP,VANCOUVER V6T 1W5,BC,CANADA. HANOVER MED SCH,ZENTRUM INNERE MED,W-3000 HANNOVER 61,GERMANY. RP KLEIN, HG (reprint author), NHLBI,MOLEC DIS BRANCH,BLDG 10,ROOM 7N117,BETHESDA,MD 20892, USA. NR 53 TC 44 Z9 46 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 1992 VL 89 IS 2 BP 499 EP 506 DI 10.1172/JCI115612 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HD270 UT WOS:A1992HD27000021 PM 1737840 ER PT J AU SHIRAI, A COSENTINO, M LEITMANKLINMAN, SF KLINMAN, DM AF SHIRAI, A COSENTINO, M LEITMANKLINMAN, SF KLINMAN, DM TI HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION INDUCES BOTH POLYCLONAL AND VIRUS-SPECIFIC B-CELL ACTIVATION SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE HIV; AIDS; B-CELLS; POLYCLONAL ACTIVATION; GP-160 ID ACQUIRED IMMUNE-DEFICIENCY; LYMPHADENOPATHY-ASSOCIATED VIRUS; SYSTEMIC LUPUS-ERYTHEMATOSUS; ANTI-LYMPHOCYTE ANTIBODIES; AIDS-RELATED COMPLEX; HOMOSEXUAL MEN; HTLV-III; LYMPHOTROPIC VIRUS; T-HELPER; OPPORTUNISTIC INFECTIONS AB Peripheral blood lymphocytes (PBL) were obtained from HIV-1-infected patients at different stages of disease. The absolute number of IgM-, IgG-, and IgA-producing lymphocytes per 10(6) PBL was increased 2.8-, 3.4-, and 1.9-fold, respectively, compared with normal controls. 2-17% of IgG-secreting patient cells reacted with the gp160 envelope glycoprotein of HIV-1 (a 737-fold increase over background), while 1-9% reacted with p24 (140-fold over background). In addition to this HIV-specific B cell activation, the number of lymphocytes reactive with nonviral antigens such as DNA, myosin, actin, trinitrophenylated keyhole limpet hemocyanin, and ovalbumin was increased by a mean of 17.9-fold. Evidence suggests that the latter changes reflect an HIV-induced polyclonal B cell activation unrelated to the production of anti-HIV antibodies. For example, the proportion of IgG anti-gp160- and anti-p24-secreting lymphocytes declined in patients with advanced disease, whereas the number of B cells producing antibodies to non-HIV antigens rose. Moreover, CD4 cell count and T4/T8 ratio showed a significant inverse correlation with the degree of polyclonal activation but not with anti-HIV responsiveness. These observations demonstrate that both quantitative and qualitative changes in B cell activation accompany (and may be predictive of) disease progression in HIV-infected individuals. C1 US FDA,CTR BIOL,DIV VIROL,RETROVIRUS RES LAB,BETHESDA,MD 20014. NIH,DEPT TRANSFUS MED,APHORESIS SECT,BETHESDA,MD 20892. NR 69 TC 159 Z9 161 U1 0 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 1992 VL 89 IS 2 BP 561 EP 566 DI 10.1172/JCI115621 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HD270 UT WOS:A1992HD27000030 PM 1737846 ER PT J AU KIDA, Y RAZ, I MAEDA, R NYOMBA, BL STONE, K BOGARDUS, C SOMMERCORN, J MOTT, DM AF KIDA, Y RAZ, I MAEDA, R NYOMBA, BL STONE, K BOGARDUS, C SOMMERCORN, J MOTT, DM TI DEFECTIVE INSULIN-RESPONSE OF PHOSPHORYLASE-PHOSPHATASE IN INSULIN-RESISTANT HUMANS SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE GLUCOSE DISPOSAL; GLYCOGEN SYNTHASE; OKADAIC ACID; SKELETAL MUSCLE; TYPE-1 PROTEIN PHOSPHATASE ID RABBIT SKELETAL-MUSCLE; GLYCOGEN-SYNTHASE PHOSPHATASE; DEPENDENT PROTEIN-KINASE; PHOSPHOPROTEIN PHOSPHATASE; CELLULAR-REGULATION; MAMMALIAN-TISSUES; BINDING SUBUNIT; ACTIVATION; METABOLISM; GLUCOSE AB Insulin-stimulated glycogen synthase activity in human muscle is reduced in insulin-resistant subjects. Insulin regulation of human muscle glycogen synthase may require activation of a type-1 protein phosphatase (PP-1). We investigated the change of phosphorylase phosphatase and glycogen synthase activities in muscle biopsies obtained during a 2-h hyperinsulinemic euglycemic clamp in 12 insulin-sensitive (group S) and 8 insulin-resistant (group R) subjects. Fasting phosphorylase phosphatase activity was lower in group R than in group S, and did not increase significantly with insulin infusion in group R until 20 min. In group S, phosphorylase phosphatase was significantly stimulated by 10 min, remaining significantly higher than in group R at all time points. The insulin-mediated changes in phosphatase activities were not decreased by 3 nM okadaic acid but were completely inhibited by 1-mu-M okadaic acid, thereby verifying that insulin-stimulated phosphorylase phosphatase is accounted for by a PP-1. Subcellular fractionation demonstrated reduced fasting PP-1 activities in both the glycogen and cytosolic fractions of muscle obtained from subjects in group R compared to those in group S. These results suggest that insulin activation of PP-1 could contribute to the stimulation of glycogen synthase by this hormone in human muscle. Lower fasting PP-1 activity in cytosol and glycogen fractions plus lower insulin-stimulated PP-1 activity could explain, in part, reduced insulin-stimulated glycogen synthase in skeletal muscle of insulin-resistant subjects. C1 NIDDKD,CLIN DIABET & NUTR SECT,ROOM 541,4212 N 16TH ST,PHOENIX,AZ 85016. NR 50 TC 36 Z9 36 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 1992 VL 89 IS 2 BP 610 EP 617 DI 10.1172/JCI115627 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HD270 UT WOS:A1992HD27000036 PM 1737850 ER PT J AU FELIX, CA NAU, MM TAKAHASHI, T MITSUDOMI, T CHIBA, I POPLACK, DG REAMAN, GH COLE, DE LETTERIO, JJ WHANGPENG, J KNUTSEN, T MINNA, JD AF FELIX, CA NAU, MM TAKAHASHI, T MITSUDOMI, T CHIBA, I POPLACK, DG REAMAN, GH COLE, DE LETTERIO, JJ WHANGPENG, J KNUTSEN, T MINNA, JD TI HEREDITARY AND ACQUIRED P53 GENE-MUTATIONS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE B-CELL PRECURSOR; GERMLINE; LI-FRAUMENI SYNDROME; T-CELL; TUMOR SUPPRESSOR GENE ID POLYMERASE CHAIN-REACTION; CELLULAR TUMOR-ANTIGEN; POINT MUTATIONS; LUNG-CANCER; T-CELL; BREAST-CANCER; BLAST CRISIS; ONCOGENE; DNA; CHROMOSOME-17 AB The p53 gene was examined in primary lymphoblasts of 25 pediatric patients with acute lymphoblastic leukemia by the RNase protection assay and by single strand conformation polymorphism analysis in 23 of 25 cases. p53 mutations were found to occur, but at a low frequency (4 of 25). While all four mutations were identified by single strand conformation polymorphism, the comparative sensitivity of RNase protection was 50% (2 of 4). Heterozygosity was retained at mutated codons in 3 of 4 cases. One pedigree was consistent with the Li-Fraumeni syndrome, and bone marrow from both diagnosis and remission indicated a germline G to T transversion at codon 272 (valine to leucine). Although members of another family were affected with leukemia, a 2-bp deletion in exon 6 was nonhereditary. The other two nonhereditary p53 mutations included a T to G transversion at codon 270 (phenylalanine to cysteine) and a G to C transversion at codon 248 (arginine to proline). These data support the role of both hereditary and acquired p53 mutations in the pathogenesis and/or progression of some cases of childhood acute lymphoblastic leukemia. C1 NCI,PEDIAT BRANCH,BETHESDA,MD 20892. NCI,USN,MED ONCOL BRANCH,BETHESDA,MD 20892. NCI,MED BRANCH,BETHESDA,MD 20892. CHILDRENS HOSP,NATL MED CTR,DIV HEMATOL ONCOL,WASHINGTON,DC 20010. GEORGE WASHINGTON UNIV,SCH MED,DEPT PEDIAT,WASHINGTON,DC 20010. UNIV TEXAS,HLTH SCI CTR,SW MED SCH,SIMMONS CANC CTR,DALLAS,TX 75235. RI Takahashi, Takashi/I-7262-2014; OI Mitsudomi, Tetsuya/0000-0001-9860-8505 NR 52 TC 95 Z9 98 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 1992 VL 89 IS 2 BP 640 EP 647 DI 10.1172/JCI115630 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA HD270 UT WOS:A1992HD27000039 PM 1737852 ER PT J AU LUNDGREN, B KOVACS, JA NELSON, NN STOCK, F MARTINEZ, A GILL, VJ AF LUNDGREN, B KOVACS, JA NELSON, NN STOCK, F MARTINEZ, A GILL, VJ TI PNEUMOCYSTIS-CARINII AND SPECIFIC FUNGI HAVE A COMMON EPITOPE, IDENTIFIED BY A MONOCLONAL-ANTIBODY SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID PNEUMONIA; EXPRESSION; DIAGNOSIS; SEQUENCE; PROTEINS; GENE AB Because Pneumocystis carinii may be related to fungi, we evaluated the reactivities of monoclonal antibodies raised against P. carinii with a variety of fungi. Fifty-two fungi and six protozoa were evaluated by immunofluorescence. One of three monoclonal antibodies (MAbs) tested (MAb 7D7) reacted with 15 fungi but no protozoa. Saccharomyces cerevisiae showed the strongest reactivity by immunofluorescence. The reactive antigen was characterized for four fungi by the immunoblot technique. In all cases the antigen that was reactive with MAb 7D7 was larger than the P. carinii antigens that reacted with 7D7. In further studies with P. carinii, Aspergillus species, and S. cerevisiae, we found that MAb 7D7 reacted with a carbohydrate component in all organisms. The presence of an epitope that is common to P. carinii and a number of fungi further supports the fungal nature of P. carinii. C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,BETHESDA,MD 20892. NIH,WARREN GRANT MAGNUSON CLIN CTR,MICROBIOL SERV,BETHESDA,MD 20892. NR 23 TC 20 Z9 20 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 1992 VL 30 IS 2 BP 391 EP 395 PG 5 WC Microbiology SC Microbiology GA HA100 UT WOS:A1992HA10000024 PM 1371519 ER PT J AU KOSINSKI, RM AXELROD, P REX, JH BURDAY, M SIVAPRASAD, R WREIOLE, A AF KOSINSKI, RM AXELROD, P REX, JH BURDAY, M SIVAPRASAD, R WREIOLE, A TI SPOROTHRIX-SCHENCKII FUNGEMIA WITHOUT DISSEMINATED SPOROTRICHOSIS SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Note ID BLOOD CULTURES; DISSEMINATED HISTOPLASMOSIS; TRANSPLANT RECIPIENT; FILAMENTOUS FUNGI; INFECTION; RECOVERY; ISOLATOR; YEASTS; SYSTEM AB Fungemia is a rare complication of Sporothrix schenckii infection and has always been associated with disseminated sporotrichosis. We describe an immunocompetent patient with localized lymphocutaneous sporotrichosis from whose blood the fungus was isolated. A lysis-centrifugation blood culture system may have improved our ability to detect low-level S. schenckii fungemia. C1 MONMOUTH MED CTR,DEPT INTERNAL MED,MONMOUTH,NJ 07740. MONMOUTH MED CTR,DIV INFECT DIS,MONMOUTH,NJ 07740. MONMOUTH MED CTR,CLIN MICROBIOL LAB,MONMOUTH,NJ 07740. TEMPLE UNIV,DEPT MED,INFECT DIS SECT,PHILADELPHIA,PA 19122. NIAID,CLIN INVEST LAB,BETHESDA,MD 20892. NR 36 TC 8 Z9 8 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 1992 VL 30 IS 2 BP 501 EP 503 PG 3 WC Microbiology SC Microbiology GA HA100 UT WOS:A1992HA10000044 PM 1537924 ER PT J AU ROSENBERG, SA AF ROSENBERG, SA TI THE IMMUNOTHERAPY AND GENE-THERAPY OF CANCER SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Editorial Material ID ACTIVATED KILLER CELLS; TUMOR-INFILTRATING LYMPHOCYTES; RECOMBINANT INTERLEUKIN-2 INVIVO; PERIPHERAL-BLOOD LYMPHOCYTES; ESTABLISHED PULMONARY METASTASES; PURIFIED HUMAN INTERLEUKIN-2; MURINE HEPATIC METASTASES; HIGH-DOSE INTERLEUKIN-2; HUMAN SOLID TUMORS; PHASE-II TRIAL RP ROSENBERG, SA (reprint author), NCI,SURG,BETHESDA,MD 20892, USA. NR 148 TC 425 Z9 429 U1 2 U2 5 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD FEB PY 1992 VL 10 IS 2 BP 180 EP 199 PG 20 WC Oncology SC Oncology GA HB270 UT WOS:A1992HB27000003 PM 1732421 ER PT J AU LONGO, DL DUFFEY, PL YOUNG, RC HUBBARD, SM IHDE, DC GLATSTEIN, E PHARES, JC JAFFE, ES URBA, WJ DEVITA, VT AF LONGO, DL DUFFEY, PL YOUNG, RC HUBBARD, SM IHDE, DC GLATSTEIN, E PHARES, JC JAFFE, ES URBA, WJ DEVITA, VT TI CONVENTIONAL-DOSE SALVAGE COMBINATION CHEMOTHERAPY IN PATIENTS RELAPSING WITH HODGKINS-DISEASE AFTER COMBINATION CHEMOTHERAPY - THE LOW PROBABILITY FOR CURE SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID BONE-MARROW TRANSPLANTATION; RADIATION-THERAPY; ETOPOSIDE; SURVIVAL C1 NCI,DIV CANC TREATMENT,BETHESDA,MD 20892. RP LONGO, DL (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,BETHESDA,MD 20892, USA. NR 24 TC 299 Z9 304 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD FEB PY 1992 VL 10 IS 2 BP 210 EP 218 PG 9 WC Oncology SC Oncology GA HB270 UT WOS:A1992HB27000005 PM 1732422 ER PT J AU GOFFMAN, TE DACHOWSKI, LJ BOBO, H OLDFIELD, EH STEINBERG, SM COOK, J MITCHELL, JB KATZ, D SMITH, R GLATSTEIN, E AF GOFFMAN, TE DACHOWSKI, LJ BOBO, H OLDFIELD, EH STEINBERG, SM COOK, J MITCHELL, JB KATZ, D SMITH, R GLATSTEIN, E TI LONG-TERM FOLLOW-UP ON NATIONAL-CANCER-INSTITUTE PHASE-I PHASE-II STUDY OF GLIOBLASTOMA-MULTIFORME TREATED WITH IODODEOXYURIDINE AND HYPERFRACTIONATED IRRADIATION SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID BRAIN-TUMORS; CELLULAR RADIOSENSITIZATION; 5-BROMOURACIL SUBSTITUTION; CHEMICAL BASIS; RADIOTHERAPY; CELLS; DNA; SENSITIVITY; RADIOLYSIS; GLIOMA C1 CANC NURSING SERV,CTR CLIN,DEPT NURSING,BETHESDA,MD. NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892. NINCDS,OFF CLIN DIRECTOR,BETHESDA,MD 20892. RP GOFFMAN, TE (reprint author), NCI,RADIAT ONCOL BRANCH,B3B69 BLDG 10,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 30 TC 50 Z9 51 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD FEB PY 1992 VL 10 IS 2 BP 264 EP 268 PG 5 WC Oncology SC Oncology GA HB270 UT WOS:A1992HB27000012 PM 1310102 ER PT J AU WEISS, GR MARGOLIN, KA ARONSON, FR SZNOL, M ATKINS, MB DUTCHER, JP GAYNOR, ER BOLDT, DH DOROSHOW, JH BAR, MH HAWKINS, MJ DEMCHAK, PA GUCALP, R FISHER, RI AF WEISS, GR MARGOLIN, KA ARONSON, FR SZNOL, M ATKINS, MB DUTCHER, JP GAYNOR, ER BOLDT, DH DOROSHOW, JH BAR, MH HAWKINS, MJ DEMCHAK, PA GUCALP, R FISHER, RI TI A RANDOMIZED PHASE-II TRIAL OF CONTINUOUS INFUSION INTERLEUKIN-2 OR BOLUS INJECTION INTERLEUKIN-2 PLUS LYMPHOKINE-ACTIVATED KILLER-CELLS FOR ADVANCED RENAL-CELL CARCINOMA SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID RECOMBINANT INTERLEUKIN-2; ADOPTIVE IMMUNOTHERAPY; ADVANCED CANCER; LYMPHOCYTES C1 NCI,DIV CANC THERAPY,INVEST DRUG BRANCH,CANC THERAPY EVALUAT PROGRAM,BETHESDA,MD 20892. CITY HOPE CANC RES CTR,DUARTE,CA. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. TUFTS UNIV,NEW ENGLAND MED CTR,BOSTON,MA 02111. ALBERT EINSTEIN CANC CTR,NEW YORK,NY. LOYOLA UNIV,MED CTR,MAYWOOD,IL 60153. RP WEISS, GR (reprint author), UNIV TEXAS,HLTH SCI CTR,AUDIE L MURPHY MEM VET ADM HOSP,DEPT MED & MED ONCOL,SAN ANTONIO,TX 78284, USA. FU NCI NIH HHS [N01-CM73702, N01-CM73703, N01-CM73707] NR 11 TC 125 Z9 124 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD FEB PY 1992 VL 10 IS 2 BP 275 EP 281 PG 7 WC Oncology SC Oncology GA HB270 UT WOS:A1992HB27000014 PM 1732429 ER PT J AU PIGOTT, TA LHEUREUX, F HILL, JL BIHARI, K BERNSTEIN, SE MURPHY, DL AF PIGOTT, TA LHEUREUX, F HILL, JL BIHARI, K BERNSTEIN, SE MURPHY, DL TI A DOUBLE-BLIND-STUDY OF ADJUVANT BUSPIRONE HYDROCHLORIDE IN CLOMIPRAMINE-TREATED PATIENTS WITH OBSESSIVE-COMPULSIVE DISORDER SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Article ID PLACEBO-CONTROLLED TRIAL; SEROTONERGIC RESPONSIVITY; AUGMENTATION; FLUVOXAMINE; FLUOXETINE; FENFLURAMINE; SERTRALINE; TRYPTOPHAN; LITHIUM AB In this study, 14 patients with obsessive-compulsive disorder (OCD) who had received at least 3 months of treatment with clomipramine were treated with the anxiolytic agent buspirone in a 10-week, double-blind study. Before the addition of buspirone, these patients as a group had shown a partial but incomplete reduction (averaging 28%) in OCD symptoms during clomipramine treatment alone. Because buspirone has been reported to be efficacious as a sole agent and as an adjunct agent in combination with fluoxetine in patients with OCD, we were interested in assessing whether buspirone added to clomipramine treatment would be associated with further significant reductions in OCD or depressive symptoms. Although adjuvant buspirone treatment was well tolerated in most subjects, mean OCD and depressive symptoms, as evaluated by standardized rating scales, did not significantly change from baseline scores achieved on clomipramine treatment alone, either after the addition of placebo for 2 weeks or buspirone (57 +/- 7 mg/day) for an additional 10 weeks. However on an individual basis, 4 (29%) of the 14 patients did have an additional 25% reduction in OCD symptoms after adjuvant buspirone treatment. This double-blind study suggests that adjunctive buspirone therapy is not generally associated with significant further clinical improvement in OCD or depressive symptoms compared with clomipramine monotherapy, but that there may be a subgroup of patients who do benefit from adjuvant buspirone therapy. C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 46 TC 105 Z9 107 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD FEB PY 1992 VL 12 IS 1 BP 11 EP 18 PG 8 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HB350 UT WOS:A1992HB35000003 PM 1552034 ER PT J AU GRADY, TA PIGOTT, TA LHEUREUX, F MURPHY, DL AF GRADY, TA PIGOTT, TA LHEUREUX, F MURPHY, DL TI SEIZURE ASSOCIATED WITH FLUOXETINE AND ADJUVANT BUSPIRONE THERAPY SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Letter ID OBSESSIVE-COMPULSIVE DISORDER; DRUG-DRUG INTERACTIONS; CLOMIPRAMINE RP GRADY, TA (reprint author), NIMH,CLIN SCI LAB,BETHESDA,MD 20892, USA. NR 10 TC 18 Z9 18 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD FEB PY 1992 VL 12 IS 1 BP 70 EP 71 DI 10.1097/00004714-199202000-00027 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HB350 UT WOS:A1992HB35000019 PM 1552045 ER PT J AU POST, RM WEISS, SRB CHUANG, DM AF POST, RM WEISS, SRB CHUANG, DM TI MECHANISMS OF ACTION OF ANTICONVULSANTS IN AFFECTIVE-DISORDERS - COMPARISONS WITH LITHIUM SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Article ID GAMMA-AMINOBUTYRIC-ACID; VOLTAGE-DEPENDENT LIMITATION; RECEPTOR-IONOPHORE COMPLEX; CENTRAL NERVOUS-SYSTEM; MOUSE CENTRAL NEURONS; TERM FOLLOW-UP; RAT-BRAIN; BENZODIAZEPINE RECEPTOR; CHRONIC CARBAMAZEPINE; VALPROIC ACID AB Until recently it appeared that lithium carbonate possessed a unique spectrum of clinical action in the acute and prophylactic treatment of manic and depressive episodes. It is now increasingly apparent that the anticonvulsants carbamazepine and valproate also share components of this spectrum of efficacy in the affective disorders but, in addition, are clinically effective in some lithium nonresponders. This clinical convergence can now drive a reexamination of the potential mechanisms of action of these compounds in the affective disorders. In spite of intensive study over several decades, the mechanism of action of lithium has remained elusive. A basic conundrum in the consideration of the actions of lithium has also been to explain how a simple ion could have such complex effects on multiple neurotransmitter systems and, in particular, have bimodal actions in the treatment of both manic and depressive phases of the illness. We suggest that a fundamental reconceptualization of both mania and depression as overactivated neural systems (either excitatory or inhibitory) could facilitate this conceptualization. Given the recent evidence linking lithium's effects to uncoupling receptor-mediated activity at the level of G-proteins or attenuating it at the level of second messenger systems mediated by adenylate cyclase or phosphoinositide turnover, these mechanisms become ideal candidates for considering how the drug could dampen overactivated systems potentially relevant to either depression or mania. The lag in onset of maximum therapeutic action of lithium, carbamazepine, and valproate further suggests that biologic effects associated with chronic compared with acute administration are the prime candidates for psychotropic effects. Comparison of the acute and chronic effects of carbamazepine with those of valproate is also offered to focus on the most likely receptor, second-messenger, and ion channel mechanisms involved in their anticonvulsant and psychotropic actions. It is hoped that better understanding of the comparative actions of lithium, carbamazepine, and valproate will allow better targeting of individual drugs for individual patients as well as, ultimately, the development of new and more selective treatments for the recurrent affective disorders. RP POST, RM (reprint author), NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,ROOM 3N212,BETHESDA,MD 20892, USA. NR 135 TC 54 Z9 55 U1 0 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD FEB PY 1992 VL 12 IS 1 SU S BP S23 EP S35 PG 13 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HB547 UT WOS:A1992HB54700005 PM 1541715 ER PT J AU POTTER, WZ BOWDEN, CL AF POTTER, WZ BOWDEN, CL TI VALPROATE AND MOOD DISORDERS - PERSPECTIVES, DERIVED FROM THE SUMMIT CONFERENCES ON THE TREATMENT OF BIPOLAR DISORDERS, JULY 27-28, 1990, COLORADO-SPRINGS, COLORADO AND JANUARY 24-27, 1991, SNOWMASS, COLORADO - INTRODUCTION SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Editorial Material ID MANIC-DEPRESSIVE ILLNESS; MIXED AFFECTIVE STATES; FOLLOW-UP; LITHIUM PROPHYLAXIS; ANTI-DEPRESSANTS; CARBONATE; DIAGNOSIS C1 UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,DIV BIOL PSYCHIAT,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284. NIMH,CLIN PHARMACOL SECT,BETHESDA,MD 20892. NR 37 TC 4 Z9 4 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD FEB PY 1992 VL 12 IS 1 SU S BP S2 EP S6 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA HB547 UT WOS:A1992HB54700001 PM 1541714 ER PT J AU ROBINSON, G EVANS, JJ CATT, KJ AF ROBINSON, G EVANS, JJ CATT, KJ TI OXYTOCIN STIMULATES LH-PRODUCTION BY THE ANTERIOR-PITUITARY GLAND OF THE RAT SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID GONADOTROPIN-RELEASING HORMONE; HYPOPHYSEAL PORTAL BLOOD; LUTEINIZING-HORMONE; BIOSYNTHESIS; CELLS; VASOPRESSIN; BRATTLEBORO AB Gonadotrophin-releasing activity of oxytocin has previously been demonstrated in vitro and in vivo. This study investigated whether oxytocin is also able to induce LH accumulation in pituitary cells. Following trypsin digestion and mechanical dispersion, pituitary cells from female rats were incubated with oxytocin (100 nmol/l) for 24 h. LH release stimulated by oxytocin increased (P < 0.001) progressively during the incubation indicating a different secretory pattern from the more rapid but less sustained secretion stimulated by gonadotrophin-releasing hormone. Oxytocin also enhanced (P < 0.01) total LH accumulation in the incubation system (released plus cell contents) which was apparent after 7-11 h of stimulation. The release of LH stimulated by oxytocin was reduced by the protein synthesis inhibitor cycloheximide (10-mu-mol/l). However, cycloheximide did not completely block oxytocin-stimulated LH release; there remained some LH release above that seen in non-stimulated controls (P < 0.01) revealing the presence of a cycloheximide-resistant component in the release mechanism. Furthermore, accumulation of total LH in 24 h incubations was suppressed (P < 0.01) by cycloheximide. The advancement in LH release which oxytocin has been shown to induce in vivo in pro-oestrous rats was accompanied by an early reduction of pituitary LH stores. However, the fall normally observed in LH content during the surge was markedly attenuated by the oxytocin treatment. Thus, loss of pituitary LH stores was less in oxytocin-treated rats than in saline-treated controls, even though net LH release into plasma was increased. Therefore, oxytocin stimulated the replenishment of LH stores. Although the mechanism(s) remains to be defined and the relationships between in-vitro and in-vivo results are as yet uncharacterized, the present study demonstrates that oxytocin treatment stimulates LH production in both dispersed cells and intact pituitaries in situ. C1 UNIV CANTERBURY,CHRISTCHURCH WOMENS HOSP,DEPT OBSTET & GYNAECOL,CHRISTCHURCH 1,NEW ZEALAND. NICHHD,ENDOCRINOL & REPROD RES BRANCH,BETHESDA,MD 20892. NR 21 TC 23 Z9 23 U1 0 U2 0 PU J ENDOCRINOLOGY LTD PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS, ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ SN 0022-0795 J9 J ENDOCRINOL JI J. Endocrinol. PD FEB PY 1992 VL 132 IS 2 BP 277 EP 283 DI 10.1677/joe.0.1320277 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA HD169 UT WOS:A1992HD16900013 PM 1541926 ER PT J AU ROCK, CD HEATH, TG ZEEVAART, JAD AF ROCK, CD HEATH, TG ZEEVAART, JAD TI 2-TRANS-ABSCISIC ACID BIOSYNTHESIS AND METABOLISM OF ABA-ALDEHYDE AND XANTHOXIN IN WILD-TYPE AND THE ABA MUTANT OF ARABIDOPSIS-THALIANA SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE ABA-ALCOHOL; ABA-ALDEHYDE; ABA-GLUCOSE ESTER; (O2)O-18 LABELING; PHASEIC ACID ID CELL-FREE PREPARATIONS; ABSCISIC-ACID; MOLECULAR-OXYGEN; TOMATO; FLACCA; FRAGMENTATION; ACCUMULATION; IONIZATION; CONVERSION; SITIENS AB Abscisic acid (ABA) and 2-trans-ABA (t-ABA) biosynthesis were studied in wild type Landsberg erecta and the three allelic aba mutants of Arabidopsis thaliana (L.) Heynh., which are impaired in epoxy-carotenoid biosynthesis. Labelling experiments with O-18(2) and mass spectrometric analysis of [O-18]ABA and its catabolites ABA-glucose ester (ABA-GE) and phaseic acid (PA), and t-ABA and t-ABA-GE, showed that t-ABA biosynthesis was less affected than ABA biosynthesis by mutations at the ABA locus. The aba-4 allele caused the most severe impairment of ABA biosynthesis compared with the other two mutant alleles aba-1 and aba-3, yet aba-4 plants synthesized as much t-ABA as wild type Landsberg erecta plants. Feeding experiments with RS-[H-2(6)]ABA-aldehyde isomers and unlabelled xanthoxin isomers suggest that t-xanthoxin and t-ABA-aldehyde are precursors to ABA and t-ABA in Arabidopsis. C1 MICHIGAN STATE UNIV,MUS DOE PLANT RES LAB,E LANSING,MI 48824. MICHIGAN STATE UNIV,NIH,FACIL MASS SPECTROMETRY,E LANSING,MI 48824. NR 27 TC 8 Z9 8 U1 1 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 1992 VL 43 IS 247 BP 249 EP 256 DI 10.1093/jxb/43.2.249 PG 8 WC Plant Sciences SC Plant Sciences GA HE546 UT WOS:A1992HE54600016 ER PT J AU KARP, DR LONG, EO AF KARP, DR LONG, EO TI IDENTIFICATION OF HLA-DR1 BETA-CHAIN RESIDUES CRITICAL FOR BINDING STAPHYLOCOCCAL ENTEROTOXIN-A AND ENTEROTOXIN-E SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID SHOCK SYNDROME TOXIN-1; CLASS-II MOLECULES; T-CELLS; HLA-DR; NUCLEOTIDE-SEQUENCE; SUPERANTIGENS; SPECIFICITY; STIMULATION; RECEPTORS; PROTEINS AB Superantigens are thought to make external contacts with major histocompatibility complex (MHC) class II molecules and with the V-beta portion of a T cell antigen receptor (TCR), thereby stimulating entire families of T cells. The precise mapping of superantigen binding sites on class II molecules may provide valuable information on how TCR and MHC molecules interact. Two bacterial superantigens, staphylococcal enterotoxins A and E (SEA/SEE) bind well to most HLA-DR alleles, but poorly to HLA-DRw53. The sequences responsible for this binding were localized to the putative alpha-helix of the DR beta-chain by measuring toxin binding to a panel of chimeric class II molecules expressed on transfected cells. Binding of SEA/SEE to the DRw14 (Dw9) molecule suggested that the conserved histidine 81 in the beta-chain of most DR molecules was important, whereas the tyrosine 81 in the DRw53 beta-chain was detrimental for high-affinity binding. To prove this, reciprocal point mutations were introduced in the DR1 and DRw53 beta-chains. Mutation of histidine 81 in the DR1 beta-chain to tyrosine reduced SEA/SEE binding, but did not prevent recognition of two DR1-restricted peptides by six of eight antigen-specific T cell lines. Conversely, introduction of histidine at position 81 in the DRw53 beta-chain restored normal levels of SEA/SEE binding. These data suggest that a binding site of SEA and SEE lies on the outer face of the beta-chain alpha-helix, pointing away from the antigen-binding groove. C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. RP KARP, DR (reprint author), UNIV TEXAS,SW MED CTR,SIMMONS ARTHRIT RES CTR,DALLAS,TX 75235, USA. RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 NR 43 TC 124 Z9 124 U1 0 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD FEB 1 PY 1992 VL 175 IS 2 BP 415 EP 424 DI 10.1084/jem.175.2.415 PG 10 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA HB061 UT WOS:A1992HB06100012 PM 1370684 ER PT J AU EISENLOHR, LC YEWDELL, JW BENNINK, JR AF EISENLOHR, LC YEWDELL, JW BENNINK, JR TI FLANKING SEQUENCES INFLUENCE THE PRESENTATION OF AN ENDOGENOUSLY SYNTHESIZED PEPTIDE TO CYTOTOXIC LYMPHOCYTES-T SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID CLASS-I; ANTIGEN PRESENTATION; ENDOPLASMIC-RETICULUM; VIRAL PEPTIDES; CELLS; RECOGNITION; MHC; NUCLEOPROTEIN; GENE; EXPRESSION AB Cytotoxic T lymphocytes (CTL) recognize class I major histocompatibility complex molecules complexed to peptides of eight to nine residues generated from cytosolic proteins. We find that CTL recognize, in vitro and in vivo, cells synthesizing a 10-residue peptide consisting of an initiating methionine followed by nine residues corresponding to a naturally processed determinant from influenza virus nucleoprotein (NP) (residues 147-155). Addition of two COOH-terminal residues corresponding to NP residues 157 and 158 severely reduced presentation of the endogenously produced peptide to CTL in vitro and in vivo. Extension of NH2 and COOH terminal flanking residues to include residues corresponding to NP residues 137-146 and 159-168 failed to increase the antigenicity of this peptide. Its presentation was greatly enhanced, however, by further extending the NH2 and COOH termini to include all of the additional residues of NP. These findings indicate first, that a naturally processed viral ligand (with an NH2-terminal Met) of a class I molecule contains sufficient information to access intracellular class I molecules, and second, that flanking residues can influence the processing and presentation of antigens to CTL. RP EISENLOHR, LC (reprint author), NIAID,VIRAL DIS LAB,BETHESDA,MD 20892, USA. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 35 TC 196 Z9 198 U1 0 U2 3 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD FEB 1 PY 1992 VL 175 IS 2 BP 481 EP 487 DI 10.1084/jem.175.2.481 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA HB061 UT WOS:A1992HB06100019 PM 1732413 ER PT J AU KUNA, P REDDIGARI, SR RUCINSKI, D OPPENHEIM, JJ KAPLAN, AP AF KUNA, P REDDIGARI, SR RUCINSKI, D OPPENHEIM, JJ KAPLAN, AP TI MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR IS A POTENT HISTAMINE-RELEASING FACTOR FOR HUMAN BASOPHILS SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID COLONY-STIMULATING FACTOR; HUMAN MONONUCLEAR-CELLS; TUMOR NECROSIS FACTOR; MAST-CELLS; FACTOR HRF; PURIFICATION; LYMPHOCYTES; LEUKOCYTES; INVITRO; ASTHMA AB Recombinant monocyte chemotactic-activating factor (MCAF) has been shown to induce histamine release from human basophils with a dose response between 10(-9) and 10(-6) M. The peak of activity was reached at 10(-7) M. Histamine release by MCAF was rapid with an initial rate comparable with histamine release by an optimal dose of anti-IgE. MCAF led to peak histamine release within 1 min. 80% of the subjects tested were responsive to MCAF or anti-IgE, while all were responsive to FMLP. The percentage histamine release by MCAF was, however, less than that seen with anti-IgE or FMLP, but this was attributable to a lesser percent release in nonatopic subjects; atopic subjects responded similarly to all three agonists. MCAF was also shown to activate highly purified human basophils more readily than mixed leukocytes, and its activity was inhibited by a polyclonal rabbit antibody. At a suboptimal concentration (2.5 x 10(-9) M), MCAF was unable to prime the basophil to histamine release by other secretagogues. However, interleukin 3 (IL-3) and IL-5 could each prime basophils for MCAF-induced secretion. Therefore, our results suggest that MCAF may be a major contributor to the histamine-releasing activity seen in peripheral blood mononuclear cell supernatants that has been designated histamine releasing factor(s). C1 NCI,BIOL RESPONSE MODIFIERS PROGRAM,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. RP KUNA, P (reprint author), SUNY STONY BROOK,HLTH SCI CTR,DEPT MED,DIV ALLERGY RHEUMATOL & CLIN IMMUNOL,STONY BROOK,NY 11794, USA. NR 28 TC 171 Z9 173 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD FEB 1 PY 1992 VL 175 IS 2 BP 489 EP 493 DI 10.1084/jem.175.2.489 PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA HB061 UT WOS:A1992HB06100020 PM 1370686 ER PT J AU MAI, UEH PEREZPEREZ, GI ALLEN, JB WAHL, SM BLASER, MJ SMITH, PD AF MAI, UEH PEREZPEREZ, GI ALLEN, JB WAHL, SM BLASER, MJ SMITH, PD TI SURFACE-PROTEINS FROM HELICOBACTER-PYLORI EXHIBIT CHEMOTACTIC ACTIVITY FOR HUMAN-LEUKOCYTES AND ARE PRESENT IN GASTRIC-MUCOSA SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID METHIONYL-LEUCYL-PHENYLALANINE; PEPTIC-ULCER DISEASE; CAMPYLOBACTER-PYLORI; ESCHERICHIA-COLI; PURIFICATION; UREASE AB The mechanism by which Helicobacter pylori, a noninvasive bacterium, initiates chronic antral gastritis in humans is unknown. We now show that H. pylori releases products with chemotactic activity for monocytes and neutrophils. This chemotactic activity was inhibited by antisera to either H. pylori whole bacteria or H. pylori-derived urease. Moreover, surface proteins extracted from H. pylori and purified H. pylori urease (a major component of the surface proteins) exhibited dose-dependent, antibody-inhibitable chemotactic activity. In addition, a synthetic 20-amino acid peptide from the NH2-terminal portion of the 61-kD subunit, but not the 30-kD subunit, of urease exhibited chemotactic activity for monocytes and neutrophils, localizing the chemotactic activity, at least in part, to the NH2 terminus of the 61-kD subunit of urease. The ability of leukocytes to chemotax to H. pylori surface proteins despite formyl-methionyl-leucyl-phenylalanine (FMLP) receptor saturation, selective inhibition of FMLP-mediated chemotaxis, or preincubation of the surface proteins with antiserum to FMLP indicated that the chemotaxis was, not FMLP mediated. Finally, we identified H. pylori surface proteins and urease in the lamina propria of gastric antra from patients with H. pylori-associated gastritis but not from uninfected subjects. These findings suggest that H. pylori gastritis is initiated by mucosal absorption of urease, which expresses chemotactic activity for leukocytes by a mechanism not involving N-formylated oligopeptides. C1 VANDERBILT UNIV,MED CTR,SCH MED,DEPT MED,DIV INFECT DIS,NASHVILLE,TN 37232. VET ADM MED CTR,NASHVILLE,TN 37232. RP MAI, UEH (reprint author), NIDR,IMMUNOL LAB,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892, USA. NR 35 TC 277 Z9 281 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD FEB 1 PY 1992 VL 175 IS 2 BP 517 EP 525 DI 10.1084/jem.175.2.517 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA HB061 UT WOS:A1992HB06100023 PM 1732414 ER EF