FN Thomson Reuters Web of Science™ VR 1.0 PT J AU KELETI, J MCCUNE, BK SZENTENDREI, T DANIELPOUR, D TSOKOS, M AF KELETI, J MCCUNE, BK SZENTENDREI, T DANIELPOUR, D TSOKOS, M TI DIFFERENTIAL TRANSFORMING GROWTH-FACTOR-BETA EXPRESSION IN NEUROBLASTOMA AND OTHER CHILDHOOD TUMORS OF NEUROECTODERMAL ORIGIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,ZENTRUM PATHOL,BETHESDA,MD 20892. NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A717 EP A717 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501138 ER PT J AU KUNDRA, V ESCOBEDO, JA KAZLAUSKAS, A KIM, HK RHEE, SG WILLIAMS, LT ZETTER, BR AF KUNDRA, V ESCOBEDO, JA KAZLAUSKAS, A KIM, HK RHEE, SG WILLIAMS, LT ZETTER, BR TI SIGNAL TRANSDUCTION PATHWAYS INVOLVED IN PDGF-STIMULATED CHEMOTAXIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 CHILDRENS HOSP MED CTR,BOSTON,MA 02115. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A642 EP A642 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500710 ER PT J AU LANE, MA BAER, D RUMPLER, WV TILMONT, EM INGRAM, DK CUTLER, RG ROTH, GS AF LANE, MA BAER, D RUMPLER, WV TILMONT, EM INGRAM, DK CUTLER, RG ROTH, GS TI LONG-TERM DIET RESTRICTION IN NONHUMAN-PRIMATES - THE NIA STUDY SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,FSK MED CTR,GERONTOL RES CTR,BALTIMORE,MD 21224. USDA,HUMAN NUTR RES CTR,BELTSVILLE,MD 20705. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A820 EP A820 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501731 ER PT J AU LARSON, DE FONTVIEILLE, AM SPRAUL, M RAVUSSIN, E AF LARSON, DE FONTVIEILLE, AM SPRAUL, M RAVUSSIN, E TI SPONTANEOUS OVERFEEDING ON A CAFETERIA DIET - EFFECTS ON 24-H SUBSTRATE OXIDATION IN LEAN AND OBESE MEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,CDNS,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A603 EP A603 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500489 ER PT J AU LEADER, JP SPRING, KR AF LEADER, JP SPRING, KR TI ELECTROPHYSIOLOGY OF THE MALPIGHIAN TUBULES OF THE ISOLATED PERFUSED MALPIGHIAN TUBULES OF THE ADULT FEMALE MOSQUITO, ANOPHELES-GAMBIAE SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV OTAGO,DEPT PHYSIOL,DUNEDIN,NEW ZEALAND. NIH,LKEM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A810 EP A810 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501675 ER PT J AU MORAN, A TURNER, RJ AF MORAN, A TURNER, RJ TI CARBACHOL (CCH) INDUCES REGULATORY VOLUME DECREASE (RVD) IN A HUMAN SALIVARY DUCTAL CELL-LINE (HSY) SO FASEB JOURNAL LA English DT Meeting Abstract C1 BEN GURION UNIV NEGEV,IL-84105 BEER SHEVA,ISRAEL. NIDR,CIPCB,BETHESDA,MD 20892. RI MORAN, ARIE/F-1210-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A811 EP A811 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501679 ER PT J AU PETERSEN, RB GOLDFARB, L TABATON, M BROWN, P LEBLANC, A MONTAGNA, P CORTELLI, P MONARI, L AUTILIOGAMBETTI, L GAJDUSEK, DC LUGARESI, E GAMBETTI, P AF PETERSEN, RB GOLDFARB, L TABATON, M BROWN, P LEBLANC, A MONTAGNA, P CORTELLI, P MONARI, L AUTILIOGAMBETTI, L GAJDUSEK, DC LUGARESI, E GAMBETTI, P TI FATAL FAMILIAL INSOMNIA AND ONE SUBTYPE OF FAMILIAL CREUTZFELD-JAKOB DISEASE - EFFECT OF A POLYMORPHISM ON A PATHOGENIC MUTATION IN THE PRION PROTEIN SO FASEB JOURNAL LA English DT Meeting Abstract C1 CASE WESTERN RESERVE UNIV,INST PATHOL,CLEVELAND,OH 44106. NIH,BETHESDA,MD 20892. RI Petersen, Robert/B-5075-2011 OI Petersen, Robert/0000-0002-3154-0072 NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A627 EP A627 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500627 ER PT J AU PORCU, P FERBER, A PIETRZKOWSKI, Z ROBERTS, CT ADAMO, M LEROITH, D BASERGA, R AF PORCU, P FERBER, A PIETRZKOWSKI, Z ROBERTS, CT ADAMO, M LEROITH, D BASERGA, R TI THE GROWTH STIMULATORY EFFECT OF SV40 T-ANTIGEN REQUIRES THE INTERACTION OF IGF-1 WITH ITS RECEPTOR SO FASEB JOURNAL LA English DT Meeting Abstract C1 THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,PHILADELPHIA,PA 19107. NIDDKD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A644 EP A644 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500723 ER PT J AU ROMANOV, V SOBEL, ME WRATHALL, L MENARD, S PINTODASILVA, P CASTRONOVO, V AF ROMANOV, V SOBEL, ME WRATHALL, L MENARD, S PINTODASILVA, P CASTRONOVO, V TI LAMININ INDUCES INTRACELLULAR-TRANSPORT OF THE 67-KD LAMININ RECEPTOR TO THE MEMBRANE IN HUMAN CANCER-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. IST NAZL TUMORI,I-20133 MILAN,ITALY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A835 EP A835 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501816 ER PT J AU ROWE, M RAVUSSIN, E COOPER, J PERRY, J PAUL, A AF ROWE, M RAVUSSIN, E COOPER, J PERRY, J PAUL, A TI MITOCHONDRIAL-DNA POLYMORPHISM ASSOCIATED WITH LOW RMR SO FASEB JOURNAL LA English DT Meeting Abstract C1 BRIGHAM YOUNG UNIV,PROVO,UT 84602. NIDDK,CDNS,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A649 EP A649 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500754 ER PT J AU SCHUBERT, RW FLETCHER, JE AF SCHUBERT, RW FLETCHER, JE TI IS MYOCARDIAL TISSUE STIRRED - A THEORETICAL-EXPERIMENTAL COMPARISON SO FASEB JOURNAL LA English DT Meeting Abstract C1 LOUISIANA TECH BIOMED ENGN,RUSTON,LA 71272. NIH,APPL STUDIES LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A908 EP A908 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97502246 ER PT J AU SLAUTER, RW RAYMER, JH GAUDETTE, NF VELEZ, GR BUCHER, JR AF SLAUTER, RW RAYMER, JH GAUDETTE, NF VELEZ, GR BUCHER, JR TI MEASUREMENT OF VOLATILE ORGANIC-COMPOUNDS IN THE BREATH FOR ASSESSMENT OF EXPOSURE TO TOXIC XENOBIOTIC CHEMICALS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A703 EP A703 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501060 ER PT J AU SPRAUL, M RISING, R FONTVIEILLE, AM LARSON, DE RAVUSSIN, E AF SPRAUL, M RISING, R FONTVIEILLE, AM LARSON, DE RAVUSSIN, E TI MUSCLE SYMPATHETIC NERVOUS ACTIVITY IS POSITIVELY RELATED TO ENERGY-EXPENDITURE AND BODY-TEMPERATURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD,PHOENIX,AZ 85016. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A725 EP A725 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97501183 ER PT J AU SZALLASI, Z SMITH, CB DENNING, MF DLUGOSZ, AA YUSPA, SH PETTIT, GR BLUMBERG, PM AF SZALLASI, Z SMITH, CB DENNING, MF DLUGOSZ, AA YUSPA, SH PETTIT, GR BLUMBERG, PM TI DIFFERENTIAL REGULATION OF PROTEIN-KINASE-C ISOZYMES-ALPHA AND ISOZYMES-BETA BY BRYOSTATIN-1, 12-DEOXYPHORBOL 13-MONOESTERS AND PHORBOL 12-MYRISTATE 13-ACETATE (PMA) IN MOUSE KERATINOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A643 EP A643 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500719 ER PT J AU VIEIRA, NE ABRAMS, SA YERGEY, AL AF VIEIRA, NE ABRAMS, SA YERGEY, AL TI EXTRACTION OF MAGNESIUM FROM BIO-FLUIDS FOR USE IN HUMAN STABLE ISOTOPE MAGNESIUM KINETICS STUDIES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD 20892. BAYLOR COLL MED,USDA ARS,CHILDRENS NUTR RES CTR,HOUSTON,TX 77030. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A529 EP A529 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500063 ER PT J AU ZHANG, J HERMAN, EH FERRANS, VJ AF ZHANG, J HERMAN, EH FERRANS, VJ TI THE EFFECT OF ICRF-186 ON CARDIAC IMMUNE-RESPONSES AND MYOCYTE ALTERATIONS INDUCED BY DOXORUBICIN (DXR) IN SPONTANEOUSLY HYPERTENSIVE RATS (SHR) SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA,LAUREL,MD 20708. NHLBI,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 23 PY 1993 VL 7 IS 4 BP A685 EP A685 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP975 UT WOS:A1993KP97500959 ER PT J AU SEVERSON, RK LINET, MS AF SEVERSON, RK LINET, MS TI DOES CIGARETTE-SMOKING LEAD TO THE SUBSEQUENT DEVELOPMENT OF LEUKEMIA SO ARCHIVES OF INTERNAL MEDICINE LA English DT Editorial Material ID ADULT LEUKEMIA; RISK; ASSOCIATION; TOBACCO C1 UNIV MINNESOTA HOSP & CLIN,DIV PEDIAT ONCOL,MINNEAPOLIS,MN 55455. NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. RP SEVERSON, RK (reprint author), UNIV MINNESOTA HOSP & CLIN,DIV EPIDEMIOL,BOX 422,MINNEAPOLIS,MN 55455, USA. NR 31 TC 4 Z9 4 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9926 J9 ARCH INTERN MED JI Arch. Intern. Med. PD FEB 22 PY 1993 VL 153 IS 4 BP 425 EP 427 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA KM928 UT WOS:A1993KM92800001 PM 8435021 ER PT J AU CHERNOMORDIK, LV VOGEL, SS SOKOLOFF, A ONARAN, HO LEIKINA, EA ZIMMERBERG, J AF CHERNOMORDIK, LV VOGEL, SS SOKOLOFF, A ONARAN, HO LEIKINA, EA ZIMMERBERG, J TI LYSOLIPIDS REVERSIBLY INHIBIT CA-2+-DEPENDENT, GTP-DEPENDENT AND PH-DEPENDENT FUSION OF BIOLOGICAL-MEMBRANES SO FEBS LETTERS LA English DT Article DE MEMBRANE FUSION; LYSOLIPID; EXOCYTOSIS; VIRAL FUSION; MAST CELL; BACULOVIRUS; SEA URCHIN; MICROSOME ID SEA-URCHIN EGGS; CELL-FUSION; MAST-CELLS; EXOCYTOSIS; LYSOPHOSPHATIDYLCHOLINE; MECHANISMS; VESICLES; KINETICS; CORTICES; INVITRO AB Membrane fusion in exocytosis, intracellular trafficking, and enveloped viral infection is thought to be mediated by specialized proteins acting to merge membrane lipid bilayers. We now show that one class of naturally-occurring phospholipids, lysolipids, inhibits fusion between cell membranes, organelles, and between organelles and plasma membrane. Inhibition was reversible, did not correlate with lysis, and could be attributed to the molecular shape of lysolipids rather than to any specific chemical moiety. Fusion was arrested at a stage preceding fusion pore formation. Our results are consistent with the hypothesis that biological fusion, irrespective of trigger, involves the formation of a highly bent intermediate between membranes, the fusion stalk. RP CHERNOMORDIK, LV (reprint author), NICHHD,THEORET & PHYS BIOL LAB,BLDG 10,ROOM 6C101,BETHESDA,MD 20892, USA. RI Vogel, Steven/A-3585-2012 NR 33 TC 164 Z9 167 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD FEB 22 PY 1993 VL 318 IS 1 BP 71 EP 76 DI 10.1016/0014-5793(93)81330-3 PG 6 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA KN131 UT WOS:A1993KN13100017 PM 8436229 ER PT J AU SHRAYER, D KOUTTAB, N MAIZEL, A WANEBO, H HEARING, VJ GERSTEN, DM AF SHRAYER, D KOUTTAB, N MAIZEL, A WANEBO, H HEARING, VJ GERSTEN, DM TI GENERATION OF CYTOTOXIC ANTIBODIES TO THE B16 MURINE MELANOMA USING A FORMALINIZED VACCINE SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID TUMOR REJECTION; ANTIGENS; IDENTIFICATION; CELLS; B700; INHIBITION; EXPRESSION; PROTEINS; GROWTH AB The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor-challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B16 F10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross-reactivity with the B700 melanoma antigen. The antibodies were shown to be anti-proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement-mediated and antibody-dependent cellular cytotoxicity. Flow cytometric analyses indicated that approximately 60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines. C1 NCI,CELL BIOL LAB,BLDG 37,ROOM 1B25,BETHESDA,MD 20892. BROWN UNIV,ROGER WILLIAM MED CTR,DEPT EXPTL PATHOL,PROVIDENCE,RI 02908. BROWN UNIV,ROGER WILLIAMS MED CTR,DEPT SURG,PROVIDENCE,RI 02908. GEORGETOWN UNIV,MED CTR,DEPT PATHOL,WASHINGTON,DC 20007. FU NCI NIH HHS [CA-45148] NR 29 TC 14 Z9 14 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD FEB 20 PY 1993 VL 53 IS 4 BP 696 EP 702 DI 10.1002/ijc.2910530428 PG 7 WC Oncology SC Oncology GA KN751 UT WOS:A1993KN75100027 PM 8436442 ER PT J AU BLAISDELL, BE RUDD, KE MATIN, A KARLIN, S AF BLAISDELL, BE RUDD, KE MATIN, A KARLIN, S TI SIGNIFICANT DISPERSED RECURRENT DNA-SEQUENCES IN THE ESCHERICHIA-COLI GENOME - SEVERAL NEW GROUPS SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE STATISTICALLY SIGNIFICANTLY LONG COMMON WORDS; ESCHERICHIA-COLI, PROTEIN BINDING TRANSPORT; RHO-INDEPENDENT TRANSCRIPTION TERMINATORS; SYSTEMS INDUCIBLE BY NUTRIENT STARVATION ID EXTRAGENIC PALINDROMIC SEQUENCES; DEPENDENT TRANSPORT-SYSTEMS; NUCLEOTIDE-SEQUENCE; BACTERIAL CHROMOSOME; GENE-EXPRESSION; ORGANIZATION; EVOLUTION; FAMILY; TRANSCRIPTION; TERMINATION C1 NIH,NAT LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20892. STANFORD UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305. RP BLAISDELL, BE (reprint author), STANFORD UNIV,DEPT MATH,STANFORD,CA 94305, USA. FU NHGRI NIH HHS [HG00335-04]; NIGMS NIH HHS [GM10452-90] NR 55 TC 39 Z9 39 U1 0 U2 0 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1993 VL 229 IS 4 BP 833 EP 848 DI 10.1006/jmbi.1993.1090 PG 16 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KP939 UT WOS:A1993KP93900005 PM 8445651 ER PT J AU CASASFINET, JR SMITH, JD KUMAR, A KIM, JG WILSON, SH KARPEL, RL AF CASASFINET, JR SMITH, JD KUMAR, A KIM, JG WILSON, SH KARPEL, RL TI MAMMALIAN HETEROGENEOUS RIBONUCLEOPROTEIN-A1 AND ITS CONSTITUENT DOMAINS - NUCLEIC-ACID INTERACTION, STRUCTURAL STABILITY AND SELF-ASSOCIATION SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE HNRNP; SYNTHETIC PEPTIDES; ANNEALING SINGLE-STRANDED BINDING PROTEINS ID RNA-BINDING DOMAIN; HELIX-UNWINDING PROTEINS; MESSENGER-RNA; COMPLEX PROTEIN-A1; SYNTHETIC PEPTIDE; TERMINAL DOMAIN; HNRNP PROTEIN; CALF THYMUS; DNA; A1 C1 UNIV MARYLAND,DEPT CHEM & BIOCHEM,CATONSVILLE,MD 21228. NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 61 TC 48 Z9 48 U1 0 U2 2 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1993 VL 229 IS 4 BP 873 EP 889 DI 10.1006/jmbi.1993.1093 PG 17 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KP939 UT WOS:A1993KP93900008 PM 8445653 ER PT J AU GAUTHERET, D MAJOR, F CEDERGREN, R AF GAUTHERET, D MAJOR, F CEDERGREN, R TI MODELING THE 3-DIMENSIONAL STRUCTURE OF RNA USING DISCRETE NUCLEOTIDE CONFORMATIONAL SETS SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE RNA MODELING; CONFORMATIONAL SEARCH; NUCLEOTIDE CONFORMATION; HAIRPIN LOOPS; STRUCTURE PREDICTION ID NUCLEAR-MAGNETIC-RESONANCE; MOLECULAR MECHANICS; CRYSTAL-STRUCTURE; DOUBLE-HELIX; DNA; PROTEIN; PREDICTION; DYNAMICS; HAIRPIN; COMBINATION C1 UNIV MONTREAL,DEPT BIOCHIM,CP 6128,SUCC A,MONTREAL H3C 3J7,QUEBEC,CANADA. NIH,NATL LIBR MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894. NR 46 TC 71 Z9 71 U1 0 U2 0 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1993 VL 229 IS 4 BP 1049 EP 1064 DI 10.1006/jmbi.1993.1104 PG 16 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KP939 UT WOS:A1993KP93900019 PM 7680379 ER PT J AU KOONIN, EV AF KOONIN, EV TI A SUPERFAMILY OF ATPASES WITH DIVERSE FUNCTIONS CONTAINING EITHER CLASSICAL OR DEVIANT ATP-BINDING MOTIF SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Note DE NTP-BINDING MOTIF; NTPASE EVOLUTION; PLASMID AND CHROMOSOME PARTITIONING; NITROGENASE; ANION PUMP ID ANION-TRANSLOCATING ATPASE; ESCHERICHIA-COLI; PROTEINS; NUCLEOTIDE; SEQUENCES; GENE; SITE; PUMP RP KOONIN, EV (reprint author), NIH,NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BLDG 38A,8600 ROCKVILLE PIKE,BETHESDA,MD 20894, USA. NR 36 TC 233 Z9 233 U1 1 U2 7 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1993 VL 229 IS 4 BP 1165 EP 1174 DI 10.1006/jmbi.1993.1115 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KP939 UT WOS:A1993KP93900030 PM 8445645 ER PT J AU PAGE, ST OWEN, WC PRICE, K ELWOOD, PC AF PAGE, ST OWEN, WC PRICE, K ELWOOD, PC TI EXPRESSION OF THE HUMAN PLACENTAL FOLATE RECEPTOR TRANSCRIPT IS REGULATED IN HUMAN TISSUES - ORGANIZATION AND FULL NUCLEOTIDE-SEQUENCE OF THE GENE SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Note DE FOLATE; RECEPTOR; FOLATE-BINDING; TRANSPORT; GENE FAMILY ID AMINO-ACID-SEQUENCE; HUMAN KB CELLS; BINDING-PROTEIN; MEMBRANE; CDNA; CONSERVATION; PARTICULATE; CLONING; REGION; MILK C1 NCI,MED BRANCH,BETHESDA,MD 20892. NR 40 TC 29 Z9 30 U1 0 U2 0 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD FEB 20 PY 1993 VL 229 IS 4 BP 1175 EP 1183 DI 10.1006/jmbi.1993.1116 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KP939 UT WOS:A1993KP93900031 PM 8445646 ER PT J AU CRAGG, G AF CRAGG, G TI FAIR DEALS SO NEW SCIENTIST LA English DT Letter RP CRAGG, G (reprint author), NCI,FREDERICK,MD 21701, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW SCIENTIST PUBL EXPEDITING INC PI ELMONT PA 200 MEACHAM AVE, ELMONT, NY 11003 SN 0262-4079 J9 NEW SCI JI New Sci. PD FEB 20 PY 1993 VL 137 IS 1861 BP 51 EP 52 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KN739 UT WOS:A1993KN73900055 ER PT J AU BOJE, KM WONG, G SKOLNICK, P AF BOJE, KM WONG, G SKOLNICK, P TI DESENSITIZATION OF THE NMDA RECEPTOR COMPLEX BY GLYCINERGIC LIGANDS IN CEREBELLAR GRANULE CELL-CULTURES SO BRAIN RESEARCH LA English DT Article DE N-METHYL-D-ASPARTATE RECEPTOR; CEREBELLAR GRANULE CELL CULTURE; 1-AMINOCYCLOPROPANECARBOXYLIC ACID; DESENSITIZATION NEUROTOXICITY; HA-966 ID D-ASPARTATE RECEPTOR; SENSITIVE GLUTAMATE RECEPTORS; CENTRAL NERVOUS-SYSTEM; RAT-BRAIN; D-CYCLOSERINE; MODULATORY SITE; 7-CHLOROKYNURENIC ACID; FUNCTIONAL ANTAGONISTS; HIPPOCAMPAL-NEURONS; RECOGNITION SITES AB Glutamate neurotoxicity was examined in cultured cerebellar granule neurons following both prolonged (20-24 h) and brief (45 min) exposure to compounds acting at strychnine-insensitive glycine receptors. Glutamate neurotoxicity was reduced in a concentration-dependent fashion by brief exposure to the glycine partial agonists I-aminocyclopropanecarboxylic acid (ACPC) and (+/-)-3-amino-1-hydroxy-2-pyrrolidone (HA-966) and the competitive antagonist, 7-chlorokynurenic acid (7-CK) with a rank order efficacy: 7-CK > HA-966 > ACPC. Neither D-cycloserine (D-CS) nor glycine affected neurotoxicity produced by maximum glutamate concentrations, while glycine but not D-CS augmented the effects of submaximum glutamate concentrations. Prolonged exposure of cultures to either full (glycine) or partial agonists (ACPC, D-CS, HA-966) abolished the neuroprotective effects of ACPC and significantly diminished the neuroprotective effects of HA-966. In contrast, the neuroprotective effects of 7-CK were only marginally reduced by prolonged exposure to glycinergic ligands, while the neuroprotection afforded by compounds acting at other loci on the NMDA receptor complex (e.g. 2-amino-5-phosphonopentanoate (APV) and dizocilpine (MK-801)) were unaltered. These effects may represent homologous desensitization of the NMDA receptor complex at its strychnine-insensitive glycine receptor induced by prolonged exposure to glycinergic agonists and partial agonists. Nonetheless, levels of the NMDA receptor subunit zeta1 mRNA were unaffected by prolonged exposure to ACPC, indicating the apparent desensitization could involve a post-translational modification of the NMDA receptor complex. C1 NIDDK,LN,BLDG 8,RM 111,BETHESDA,MD 20892. NR 61 TC 66 Z9 66 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 19 PY 1993 VL 603 IS 2 BP 207 EP 214 DI 10.1016/0006-8993(93)91239-O PG 8 WC Neurosciences SC Neurosciences & Neurology GA KM986 UT WOS:A1993KM98600004 PM 8096423 ER PT J AU HILL, JM MERVIS, RF AVIDOR, R MOODY, TW BRENNEMAN, DE AF HILL, JM MERVIS, RF AVIDOR, R MOODY, TW BRENNEMAN, DE TI HIV ENVELOPE PROTEIN-INDUCED NEURONAL DAMAGE AND RETARDATION OF BEHAVIORAL-DEVELOPMENT IN RAT NEONATES SO BRAIN RESEARCH LA English DT Article DE GOLGI STAIN; DENDRITIC BRANCHING; NEURAL DEVELOPMENT; NEUROTOXICITY; ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS); PEPTIDE-T ID HUMAN-IMMUNODEFICIENCY-VIRUS; VASOACTIVE-INTESTINAL-PEPTIDE; IMMUNE-DEFICIENCY SYNDROME; GLYCOPROTEIN GP120; FRONTAL-CORTEX; INFECTION; BRAINS; CHILDREN; AIDS; ENCEPHALOPATHY AB Cognitive and motor impairment are common symptoms among patients infected with the human immunodeficiency virus (HIV), including children who suffer neurological deficits and are frequently developmentally impaired. The HIV envelope protein, gp120, which has been shown to be toxic to neurons in culture, is shed in abundance by infected cells, and thus may play a significant role in the neuropathology of AIDS. To test this possible mechanism, neonatal rats were injected systemically with purified gp120 and the following consequences were observed: (1) radiolabeled gp120 and toxic fragments thereof were recovered in brain homogenates; (2) dystrophic changes were produced in pyramidal neurons of cerebral cortex; (3) retardation was evident in developmental milestones associated with complex motor behaviors. In parallel studies, co-treatment with peptide T, a gp120-derived peptide having a pentapeptide sequence homologous with vasoactive intestinal peptide, prevented or attenuated the morphological damage and behavioral delays associated with gp120 treatment. These studies suggest that gp120 and gp120-derived toxic fragments may contribute to the neurological and neuropsychiatric impairment related to HIV infection, and that peptide T appears to be effective in preventing gp120-associated neurotoxicity in developing rodents. C1 NICHHD,DEV NEUROBIOL LAB,NEUROCHEM UNIT,BETHESDA,MD 20892. OHIO STATE UNIV,MED CTR,DIV NEUROPATHOL,COLUMBUS,OH 43210. GEORGE WASHINGTON UNIV,MED CTR,DEPT BIOCHEM,WASHINGTON,DC 20037. NR 56 TC 125 Z9 127 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 19 PY 1993 VL 603 IS 2 BP 222 EP 233 DI 10.1016/0006-8993(93)91241-J PG 12 WC Neurosciences SC Neurosciences & Neurology GA KM986 UT WOS:A1993KM98600006 PM 8461978 ER PT J AU CARUNCHO, HJ PUIA, G SLOBODYANSKY, E DASILVA, PP COSTA, E AF CARUNCHO, HJ PUIA, G SLOBODYANSKY, E DASILVA, PP COSTA, E TI FREEZE-FRACTURE IMMUNOCYTOCHEMICAL STUDY OF THE EXPRESSION OF NATIVE AND RECOMBINANT GABA(A) RECEPTORS SO BRAIN RESEARCH LA English DT Article DE GABA(A) RECEPTOR; RECEPTOR TOPOLOGY; LABEL-FRACTURE; FRACTURE-FLIP; CORTICAL NEURON; CEREBELLAR GRANULE CELL ID BENZODIAZEPINE RECEPTORS; MONOCLONAL-ANTIBODIES; AUTORADIOGRAPHIC LOCALIZATION; SUBCELLULAR-LOCALIZATION; MOLECULAR-BIOLOGY; CO-LOCALIZATION; PLASMA-MEMBRANE; A RECEPTORS; CELLS; BRAIN AB To assess the density and distribution of native and recombinant GABA(A) receptors we used label-fracture and fracture-flip technologies combined with immunocytochemistry using monoclonal and polyclonal Abs directed against the extracellular domain of the GABA(A) receptor protein located in the freeze-fracture replicas. In cortical neurons there is a high density of GABA(A) receptors on both soma and dendrites with some areas were the density of receptors is higher, but there are no well defined clusters. In cerebellar granule cells most of the receptors are distributed in round clusters both in neurites and soma. In astroglial cells the receptor density is lower than in neurons and only occasionally they appear in clusters. In cells transfected with cDNAs encoding for various molecular forms of GABA(A) receptor subunits, the receptor density is moderate when cDNAs for alpha, beta and gamma subunits are cotransfected; however, on cells cotransfected with cDNAs for beta and gamma subunits the receptor density is significantly lower. Recombinant receptors appear randomly distributed and occasionally they aggregate in small groups. C1 GEORGETOWN UNIV,SCH MED,FIDIA GEORGETOWN INST NEUROSCI,3900 RESERVOIR RD,WASHINGTON,DC 20007. NCI,FREDERICK CANC RES & DEV CTR,MATH BIOL LAB,STRUCT BIOL SECT,FREDERICK,MD 21702. RI Puja, Giulia/C-3540-2015 OI Puja, Giulia/0000-0001-8385-6020 NR 34 TC 26 Z9 26 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 19 PY 1993 VL 603 IS 2 BP 234 EP 242 DI 10.1016/0006-8993(93)91242-K PG 9 WC Neurosciences SC Neurosciences & Neurology GA KM986 UT WOS:A1993KM98600007 PM 8384919 ER PT J AU ABASSI, ZA KEISER, HR AF ABASSI, ZA KEISER, HR TI LOSARTAN, AN ANGIOTENSIN-II INHIBITOR, DRAMATICALLY IMPROVES SODIUM-BALANCE IN RATS WITH HEART-FAILURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,HYPERTENS ENDOCRINE BRANCH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A246 EP A246 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401421 ER PT J AU ABDO, KM HAILEY, JR EUSTIS, SL HASEMAN, JK AF ABDO, KM HAILEY, JR EUSTIS, SL HASEMAN, JK TI PROMETHAZINE HYDROCHLORIDE, A NON-CARCINOGEN FOR RATS AND MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A470 EP A470 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402720 ER PT J AU ABRAMS, SA STUFF, J VIEIRA, NE YERGEY, AL AF ABRAMS, SA STUFF, J VIEIRA, NE YERGEY, AL TI CALCIUM BALANCE AND KINETIC-PARAMETERS DEMONSTRATE A PEAK PERIOD OF MINERAL ACCRETION IN EARLY ADOLESCENCE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD 20892. BAYLOR COLL MED,USDA ARS,CHILDRENS NUTR RES CTR,HOUSTON,TX 77030. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A67 EP A67 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400387 ER PT J AU ALGER, S SEAGLE, H RAVUSSIN, E AF ALGER, S SEAGLE, H RAVUSSIN, E TI FOOD-INTAKE, ENERGY-EXPENDITURE IN OBESE FEMALE BINGERS AND NON-BINGERS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD,CDNS,PHOENIX,AZ. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A389 EP A389 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402246 ER PT J AU BACIC, M WOLOZIN, B MERRILL, MJ AF BACIC, M WOLOZIN, B MERRILL, MJ TI IMMORTALIZATION OF MICROVASCULAR ENDOTHELIAL-CELLS FROM HUMAN BRAIN INDUCES VASCULAR ENDOTHELIAL GROWTH-FACTOR (VEGF) EXPRESSION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A133 EP A133 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400765 ER PT J AU BALABAN, RS TAYLOR, JF TURNER, R AF BALABAN, RS TAYLOR, JF TURNER, R TI NONINVASIVE DETECTION OF CORONARY FLOW, INVIVO, USING MAGNETIC-RESONANCE-IMAGING (MRI) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,LCE,BETHESDA,MD 20817. RI Turner, Robert/C-1820-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A423 EP A423 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402446 ER PT J AU BRODY, AR BONNER, JC LASKY, I GOODELL, A AF BRODY, AR BONNER, JC LASKY, I GOODELL, A TI UP-REGULATION OF THE ALPHA-RECEPTOR FOR PLATELET-DERIVED GROWTH-FACTOR (PDGF) AND CONTROL OF LUNG FIBROBLAST PROLIFERATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,PULM PATHOBIOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A498 EP A498 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402881 ER PT J AU CHEN, HT CHEN, G KANDASAMY, SB HARRIS, AH AF CHEN, HT CHEN, G KANDASAMY, SB HARRIS, AH TI IONIZING-RADIATION DECREASES HIPPOCAMPAL PROTEIN-KINASE-C (PKC) LEVELS IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 ARMED FORCES RADIOBIOL RES INST,DEPT BEHAV SCI,BETHESDA,MD 20889. NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A272 EP A272 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401579 ER PT J AU CONWAY, JM YANOVSKI, SZ AVILA, NA HUBBARD, VS AF CONWAY, JM YANOVSKI, SZ AVILA, NA HUBBARD, VS TI ADIPOSE-TISSUE VOLUME CHANGES DURING WEIGHT-LOSS IN BLACK-AND-WHITE FEMALES SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS,BELTSVILLE AGR RES CTR,BHNRC,ENERGY & PROT NUTR LAB,BELTSVILLE,MD 20705. NIMH,BETHESDA,MD 20892. NIADDKD,DEPT CLIN RADIOL,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A281 EP A281 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401628 ER PT J AU COWLEN, MS ELING, TE AF COWLEN, MS ELING, TE TI MODULATION OF C-JUN AND JUN-B MESSENGER-RNA AND INHIBITION OF DNA-SYNTHESIS BY PROSTAGLANDIN-E(2) IN SYRIAN-HAMSTER EMBRYO CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A43 EP A43 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400241 ER PT J AU DAVIS, CD FARB, A THORGEIRSSON, SS SNYDERWINE, EG AF DAVIS, CD FARB, A THORGEIRSSON, SS SNYDERWINE, EG TI CARDIOTOXICITY OF HETEROCYCLIC AMINE (HA) FOOD MUTAGENS IN CULTURED MYOCYTES AND IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,DCE,EXPTL CARCINOGENESIS LAB,BETHESDA,MD 20892. AFIP,DEPT CARDIOVASC PATHOL,WASHINGTON,DC 20306. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A136 EP A136 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400787 ER PT J AU DAVIS, P HORAN, PJ DECOSTA, BR RICE, KC PORRECA, F AF DAVIS, P HORAN, PJ DECOSTA, BR RICE, KC PORRECA, F TI DIFFERENCES IN OPIOID KAPPA-RECEPTOR SUBTYPES IN GUINEA-PIG ISOLATED ILEUM (GPI) AND MOUSE ISOLATED VAS-DEFERENS (MVD) SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT PHARMACOL,TUCSON,AZ 85724. NIH,ORGAN SYNTH LAB,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A489 EP A489 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402831 ER PT J AU FISHMAN, RS LARSON, DE SYSTROM, DM HROVAT, MI HESSLINK, RI AF FISHMAN, RS LARSON, DE SYSTROM, DM HROVAT, MI HESSLINK, RI TI DIETARY-EFFECTS ON EXERCISING MUSCLE METABOLISM BY P-31-MRS SO FASEB JOURNAL LA English DT Meeting Abstract C1 USN,HLTH RES CTR,SAN DIEGO,CA 92152. MGH,PULM & CRIT CARE UNIT,BOSTON,MA. MIT,FRANCIS BITTER NATL MAGNET LAB,BOSTON,MA. NIDDK,CDNS,PHOENIX,AZ. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A225 EP A225 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401297 ER PT J AU GOANS, RE ABRAMS, SA MARINI, JC VIEIRA, NE YERGEY, AL AF GOANS, RE ABRAMS, SA MARINI, JC VIEIRA, NE YERGEY, AL TI NONCOMPARTMENTAL ANALYSIS OF CALCIUM KINETICS IN NORMAL-CHILDREN AND IN CHILDREN WITH OSTEOGENESIS IMPERFECTA SO FASEB JOURNAL LA English DT Meeting Abstract C1 CNRC,HOUSTON,TX 77030. NICHHD,LTPB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A67 EP A67 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400384 ER PT J AU HEEMSKERK, FMJ ZORAD, S SELTZER, A SAAVEDRA, JM AF HEEMSKERK, FMJ ZORAD, S SELTZER, A SAAVEDRA, JM TI [I-125] CGP-42112A, A NOVEL, SPECIFIC LIGAND TO STUDY CENTRAL AND PERIPHERAL ANGIOTENSIN (AT2) RECEPTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A35 EP A35 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400200 ER PT J AU HEINEMAN, FW LAUGHLIN, MR AF HEINEMAN, FW LAUGHLIN, MR TI EFFECTS OF MALATE ASPARTATE SHUTTLE INHIBITION ON THE ENERGETICS OF THE ISOLATED, WORKING, RABBIT HEART SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A314 EP A314 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401818 ER PT J AU HONG, HL YANG, RSH BOORMAN, GA AF HONG, HL YANG, RSH BOORMAN, GA TI ENHANCEMENT OF MYELOTOXICITY INDUCED BY A MIXTURE OF GROUNDWATER CONTAMINANTS IN MICE BY REPEATED IRRADIATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,NATL TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A508 EP A508 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402942 ER PT J AU JEFFCOAT, AR GHANAYEM, BI PATRICK, DA SLAUTER, RW AF JEFFCOAT, AR GHANAYEM, BI PATRICK, DA SLAUTER, RW TI DISPOSITION OF [FE-55] FERROCENE IN RATS FOLLOWING INHALATION EXPOSURE OR INTRAVENOUS BOLUS SO FASEB JOURNAL LA English DT Meeting Abstract C1 RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A484 EP A484 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402801 ER PT J AU JEONG, KS WANG, JS VEECH, RL AF JEONG, KS WANG, JS VEECH, RL TI ORAL ETHANOL ALTERS AMINO-ACID GRADIENTS ACROSS RAT-LIVER INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,METAB & MOLEC BIOL LAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A392 EP A392 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402266 ER PT J AU KANT, AK SCHATZKIN, A AF KANT, AK SCHATZKIN, A TI DIETARY-FAT INTAKE AND WEIGHT CHANGE IN THE NHANES-I EPIDEMIOLOGIC FOLLOW-UP-STUDY (NHEFS) SO FASEB JOURNAL LA English DT Meeting Abstract C1 CUNY QUEENS COLL,FLUSHING,NY 11367. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A390 EP A390 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402253 ER PT J AU KASHIWAYA, Y TSUCHIYA, N SATO, K MASUDA, T VEECH, RL AF KASHIWAYA, Y TSUCHIYA, N SATO, K MASUDA, T VEECH, RL TI KETONE-BODIES AND INSULIN BOTH CAUSE DECREASE IN TOTAL-PI AND MOVEMENT OF INORGANIC PI FROM MITOCHONDRIA TO CYTOSOL IN THE WORKING RAT-HEART SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,ROCKVILLE,MD 20852. KITASATO UNIV,SCH MED,SAGAMIHARA,KANAGAWA 228,JAPAN. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A313 EP A313 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401812 ER PT J AU KASKOUN, MC FONTVIELLE, AM RAVUSSIN, E CARPENTER, WH POEHLMAN, ET GORAN, MI AF KASKOUN, MC FONTVIELLE, AM RAVUSSIN, E CARPENTER, WH POEHLMAN, ET GORAN, MI TI BODY-COMPOSITION OF COLOMBIAN WOMEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV VERMONT,DEPT MED,BURLINGTON,VT 05405. UNIV VERMONT,SIMS OBES NUTR RES CTR,BURLINGTON,VT 05405. NIH,CLIN DIABET & NUTR SECT,PHOENIX,AZ. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A360 EP A360 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402082 ER PT J AU KINSELLA, JL KUZUYA, M AF KINSELLA, JL KUZUYA, M TI DIFFERENTIATION OF ENDOTHELIAL-CELLS ON BASEMENT-MEMBRANE PROTEINS (MATRIGEL) - EFFECT OF CELL-CYCLE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GRC,LCS,BALTIMORE,MD 21224. NR 1 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A132 EP A132 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400761 ER PT J AU KITTUR, DS FRENCH, W STEPHENSON, R ADLER, W AF KITTUR, DS FRENCH, W STEPHENSON, R ADLER, W TI HEMIRENAL TRANSPLANTATION IN SWINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21287. NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A57 EP A57 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400322 ER PT J AU KUZUYA, M KINSELLA, JL AF KUZUYA, M KINSELLA, JL TI HUMAN SKIN FIBROBLASTS PRODUCE INVITRO ANGIOGENIC FACTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A127 EP A127 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400733 ER PT J AU LAUGHLIN, MR TAYLOR, J BALABAN, RS AF LAUGHLIN, MR TAYLOR, J BALABAN, RS TI MYOCARDIAL GLYCOGEN-SYNTHESIS IS INCREASED BY NON-GLUCOSE SUBSTRATES INVIVO IN THE DOG SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,LCE,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A314 EP A314 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401816 ER PT J AU MICHAELIS, OE ABRAHAM, AA HANSEN, CT SCHOLFIELD, DJ STRIFFLER, JS VELASQUEZ, MT AF MICHAELIS, OE ABRAHAM, AA HANSEN, CT SCHOLFIELD, DJ STRIFFLER, JS VELASQUEZ, MT TI DEVELOPMENT OF A NEW STRAIN OF OBESE HYPERINSULINEMIC SALT-SENSITIVE RAT SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE,MD 20705. GEORGE WASHINGTON UNIV,MED CTR,WASHINGTON,DC 20037. NIH,VET RES BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A270 EP A270 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401564 ER PT J AU MONI, RW DALY, JW AF MONI, RW DALY, JW TI ADENOREGULIN ENHANCES BINDING OF AGONISTS TO A(1)-ADENOSINE RECEPTORS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A462 EP A462 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402683 ER PT J AU NAVERI, L STROMBERG, C SAAVEDRA, JM AF NAVERI, L STROMBERG, C SAAVEDRA, JM TI STIMULATION OF ANGIOTENSIN-II AT(2)-RECEPTORS MODULATES THE LOWER LIMIT OF CEREBRAL BLOOD-FLOW AUTOREGULATION IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A337 EP A337 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401950 ER PT J AU NEIGHBORS, SM SLAUTER, RW GHANAYEM, BI JEFFCOAT, AR PATETTA, PK AF NEIGHBORS, SM SLAUTER, RW GHANAYEM, BI JEFFCOAT, AR PATETTA, PK TI DISPOSITION OF [C-14] METHYLCYCLOPENTADIENYL MANGANESE TRICARBONYL FOLLOWING NOSE-ONLY INHALATION AND INTRAVENOUS ADMINISTRATION TO RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 RES TRIANGLE INST,RES TRIANGLE PK,NC 27709. NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A484 EP A484 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402804 ER PT J AU OBRIEN, KO VIEIRA, NE SIUCALDERA, ML PEREZ, A HOLICK, MF YERGEY, AL ALLEN, LH AF OBRIEN, KO VIEIRA, NE SIUCALDERA, ML PEREZ, A HOLICK, MF YERGEY, AL ALLEN, LH TI HIGH-FIBER INTAKES IMPAIR CALCIUM-ABSORPTION AND ALTER CALCIUM KINETICS IN YOUNG-ADULT MEN SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CONN,STORRS,CT 06269. NICHHD,LTPB,BETHESDA,MD 20892. BOSTON UNIV,BOSTON,MA 02118. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A309 EP A309 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401787 ER PT J AU PARDHASARADHI, K KUTTY, RK KRISHNA, G AF PARDHASARADHI, K KUTTY, RK KRISHNA, G TI CDNA CLONING OF HUMAN RETINAL GUANYLATE CYCLASE-ATRIAL NATRIURETIC FACTOR RECEPTOR TYPE-A (GC-A) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NEI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A466 EP A466 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402696 ER PT J AU PARK, KS VEECH, RL SONG, BJ AF PARK, KS VEECH, RL SONG, BJ TI POSTTRANSCRIPTIONAL INDUCTION OF N-NITROSODIMETHYLAMINE DEMETHYLASE BY PYRAZINE SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA,CLIN STUDIES LAB,ROCKVILLE,MD 20852. NIAAA,DEPT METAB,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A265 EP A265 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401536 ER PT J AU PETEN, EP YANG, CW HE, CJ CAROME, MA STRIKER, GE STRIKER, LJ AF PETEN, EP YANG, CW HE, CJ CAROME, MA STRIKER, GE STRIKER, LJ TI LATE REMODELING IN GLOMERULOSCLEROSIS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK,RENAL CELL BIOL SECT,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A197 EP A197 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401139 ER PT J AU POTISCHMAN, N SWANSON, CA BRINTON, LA AF POTISCHMAN, N SWANSON, CA BRINTON, LA TI DIETARY FACTORS AND RISK OF ENDOMETRIAL CANCER SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A65 EP A65 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400374 ER PT J AU RAO, PR ABUGO, O PURI, V RIFKIND, J AF RAO, PR ABUGO, O PURI, V RIFKIND, J TI PERTURBATION OF ERYTHROCYTE-MEMBRANE PROPERTIES DUE TO PROLONGED INCUBATION IN PBS SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV MARYLAND,CATONSVILLE,MD 21228. NIA,BALTIMORE,MD 21224. UNIV MARYLAND,COLL PK,MD 20742. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A241 EP A241 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401389 ER PT J AU ROSS, SA AHRENS, RA DE LUCA, LM AF ROSS, SA AHRENS, RA DE LUCA, LM TI RETINOIC ACID UP-REGULATES THE BETA-1 INTEGRIN SUBUNIT IN F9 EMBRYONAL CARCINOMA-CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB, BETHESDA, MD 20892 USA. UNIV MD, NUTR SCI PROGRAM, COLL PK, MD 20740 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A371 EP A371 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402146 ER PT J AU RYSCHON, TW JARVIS, JC SALMONS, S BALABAN, RS AF RYSCHON, TW JARVIS, JC SALMONS, S BALABAN, RS TI P-31-NMR SPECTRA AND ISOMETRIC FORCE OF CHRONICALLY STIMULATED RABBIT MUSCLE INVIVO SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,CARDIAD ENERGET LAB,BETHESDA,MD 20892. UNIV LIVERPOOL,LIVERPOOL L69 3BX,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A225 EP A225 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401298 ER PT J AU SAWAKI, K HIRAMATSU, Y BAUM, BJ AMBUDKAR, IS AF SAWAKI, K HIRAMATSU, Y BAUM, BJ AMBUDKAR, IS TI M3-MUSCARINIC-RECEPTOR STIMULATION OF PHOSPHOINOSITIDE-SPECIFIC PLC IS MEDIATED BY GQ/11 IN RAT PAROTID-GLAND MEMBRANES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDR,CIPCB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A145 EP A145 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400837 ER PT J AU SCHUETTE, S JANGHORBANI, M ZECH, L AF SCHUETTE, S JANGHORBANI, M ZECH, L TI INVIVO MODELING OF MG KINETICS IN THE RAT USING A NOVEL STABLE ISOTOPE APPROACH SO FASEB JOURNAL LA English DT Meeting Abstract C1 BIOCHEM ANAL CORP,CHICAGO,IL 60637. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A510 EP A510 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402955 ER PT J AU SINHA, R ZIEGLER, R FREY, C MCADAMS, M NORKUS, E AF SINHA, R ZIEGLER, R FREY, C MCADAMS, M NORKUS, E TI IMPORTANCE OF SUPPLEMENTAL VITAMIN-C IN DETERMINING PLASMA ASCORBIC-ACID (AA) - IMPLICATIONS FOR EPIDEMIOLOGIC STUDIES SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. RI Sinha, Rashmi/G-7446-2015 OI Sinha, Rashmi/0000-0002-2466-7462 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A65 EP A65 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97400375 ER PT J AU SIUCALDERA, ML ALLEN, LH OBRIEN, KO YERGEY, A RAY, R HOLICK, M AF SIUCALDERA, ML ALLEN, LH OBRIEN, KO YERGEY, A RAY, R HOLICK, M TI LOW CALCIUM INTAKE REDUCES THE HALF-LIFE OF 25(OH) VITAMIN-D SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV CONNECTICUT,STORRS,CT 06268. NIH,BETHESDA,MD 20892. BOSTON UNIV,BOSTON,MA 02215. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A284 EP A284 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401646 ER PT J AU STEWART, DE PRITCHARD, JB AF STEWART, DE PRITCHARD, JB TI ORGANIC ANION TRANSPORT BY OK CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A456 EP A456 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402638 ER PT J AU STROMBERG, C NAVERI, L SAAVEDRA, JM AF STROMBERG, C NAVERI, L SAAVEDRA, JM TI STIMULATION OF ANGIOTENSIN-II AT(2)-RECEPTORS EXTENDS THE UPPER LIMIT OF CEREBRAL BLOOD-FLOW AUTOREGULATION IN RATS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A337 EP A337 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401953 ER PT J AU SZEPESI, B MICHAELIS, OE HANSEN, CT AF SZEPESI, B MICHAELIS, OE HANSEN, CT TI VERY SEVERE DIABETES IN A SUBSTRAIN OF WKY/N-CP MALE-RAT SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE,MD 20705. NIH,VET RES BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A270 EP A270 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401567 ER PT J AU VISWANATHAN, M SAAVEDRA, JM AF VISWANATHAN, M SAAVEDRA, JM TI EXPRESSION OF ANGIOTENSIN-II AT1 RECEPTORS DURING DEVELOPMENT OF NEOINTIMA IN THE CAROTID-ARTERY OF THE RAT SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A331 EP A331 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401918 ER PT J AU WILLSKARP, M CHEN, X WYNN, T AF WILLSKARP, M CHEN, X WYNN, T TI ELEVATED IL-6 MESSENGER-RNA LEVELS IN MICE GENETICALLY SUSCEPTIBLE TO OZONE-INDUCED LUNG INFLAMMATION SO FASEB JOURNAL LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A407 EP A407 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402352 ER PT J AU WU, JC MERLINO, G FAUSTO, N AF WU, JC MERLINO, G FAUSTO, N TI ESTABLISHMENT AND CHARACTERIZATION OF HEPATOCYTE LINES DERIVED FROM TRANSFORMING GROWTH FACTOR-ALPHA (TGF-ALPHA) TRANSGENIC MICE SO FASEB JOURNAL LA English DT Meeting Abstract C1 BROWN UNIV,DEPT PATHOL,PROVIDENCE,RI 02912. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A492 EP A492 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97402851 ER PT J AU YERGEY, AL ABRAMS, SA VIEIRA, NE GOANS, RE AF YERGEY, AL ABRAMS, SA VIEIRA, NE GOANS, RE TI COMPARISON OF MAGNESIUM AND CALCIUM DISTRIBUTION DYNAMICS SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHHD,BETHESDA,MD 20892. CNRC,HOUSTON,TX 77030. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 19 PY 1993 VL 7 IS 3 BP A280 EP A280 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KP974 UT WOS:A1993KP97401618 ER PT J AU BURKE, TR LIM, B MARQUEZ, VE LI, ZH BOLEN, JB STEFANOVA, I HORAK, ID AF BURKE, TR LIM, B MARQUEZ, VE LI, ZH BOLEN, JB STEFANOVA, I HORAK, ID TI BICYCLIC COMPOUNDS AS RING-CONSTRAINED INHIBITORS OF PROTEIN-TYROSINE KINASE-P56LCK SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID STYRYL-BASED INHIBITORS; GROWTH-FACTOR RECEPTOR; KINASE INHIBITORS; SIGNAL TRANSDUCTION; CELL-LINE; PHOSPHORYLATION; TYRPHOSTINS; P56LCK; LCK; TRANSFORMATION AB A study was undertaken to prepare inhibitors of the lymphocyte protein-tyrosine kinase p56lck. Using the known p56lck inhibitor 3,4-dihydroxy-alpha-cyanocinnamamide (4) as a lead compound, bicyclic analogues were designed as conformationally constrained mimetics in which the phenyl ring and vinyl side chain of the cinnamamide are locked into a coplanar orientation. Such planarity was rationalized to be an important determinant for binding within a putative flat, cleftlike catalytic cavity. Bicyclic analogues were prepared using the naphthalene, quinoline, isoquinoline, and 2-iminochromene ring systems and examined for their ability to inhibit autophosphorylation of immunopurified p56lck. The most potent analogues were methyl 7,8-dihydroxyisoquinoline-3-carboxylate (12) (IC50 = 0.2 muM) and 7,8-dihydroxyisoquinoline-3-carboxamide (13) (IC50 = 0.5 muM). Inhibition by 12 was not competitive with respect to ATP. These compounds may represent important new structural motifs for the development of p56lck inhibitors. C1 NCI,DIV CANC BIOL DIAG & CTR,METAB BRANCH,BETHESDA,MD 20892. BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC BIOL,PRINCETON,NJ 08543. RP BURKE, TR (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MED CHEM LAB,BLDG 37,RM 5C06,BETHESDA,MD 20892, USA. RI Burke, Terrence/N-2601-2014 NR 53 TC 83 Z9 83 U1 2 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD FEB 19 PY 1993 VL 36 IS 4 BP 425 EP 432 DI 10.1021/jm00056a001 PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA KN149 UT WOS:A1993KN14900001 PM 8474097 ER PT J AU ANANTHAN, S CLAYTON, SD EALICK, SE WONG, G EVONIUK, GE SKOLNICK, P AF ANANTHAN, S CLAYTON, SD EALICK, SE WONG, G EVONIUK, GE SKOLNICK, P TI SYNTHESIS AND STRUCTURE-ACTIVITY-RELATIONSHIPS OF 3,5-DISUBSTITUTED 4,5-DIHYDRO-6H-IMIDAZO[1,5-A][1,4]BENZODIAZEPIN-6-ONES AT DIAZEPAM-SENSITIVE AND DIAZEPAM-INSENSITIVE BENZODIAZEPINE RECEPTORS SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID SELECTIVE ANTAGONIST; MOLECULAR-STRUCTURE; ALCOHOL ANTAGONIST; INVERSE AGONISTS; BETA-CARBOLINE; LIGANDS; BINDING; SYSTEM; ANALOGS; DERIVATIVES AB A series of imidazobenzodiazepin-6-ones possessing varying substituents at the 3- and 5-positions were synthesized and evaluated for their affinities at diazepam-sensitive (DS) and diazepam-insensitive (DI) benzodiazepine receptors (BzR) in rat cortical and cerebellar membranes. Replacement of an ester substituent at the 3-position with a carbamate, acetylamino, formylamino, isothiocyanato, 2-oxazolinyl, 2-benzoxazolyl, or p-tolylsulfonyl groups lead to > 100-fold reductions in affinity at both DS and DI BzR. Replacement of a methyl group on the nitrogen at the 5-position with propyl, allyl, or phenethyl groups also led to significant reductions in affinity at both BzR isoforms. However, incorporation of a benzyl group yields ligands (11f,h,i and 14a-c) with moderate to high affinities at DS BzR, suggesting the presence of a hydrophobic pocket at the receptor site. Introduction of chlorine at the 7-position enhances ligand affinity at DS BzR while chlorine at the 8-position decreases affinity (IC50: 11f, 9.3 nM; 11h, 2.4 nM; 11i, 37.8 nM). In contrast, chlorine substitution at the 7-as well as the 8-position increases affinity at DI BzR (K(i): 11f, 112 nM; 11h, 20.2 nM; 11i, 10.9 nM). Compound 11i is among the few described high affinity DI-site ligands with a selectivity comparable to that of Ro 15-4513. Despite their in vitro affinities, compounds 11f, 11h, and 11i exhibit low in vivo activities that may be attributable to unfavorable metabolic or pharmacokinetic properties. C1 CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL SECT,ITHACA,NY 14853. NIDDKD,NEUROSCI LAB,BETHESDA,MD 20892. RP ANANTHAN, S (reprint author), SO RES INST,DEPT ORGAN CHEM,BIRMINGHAM,AL 35255, USA. FU NIDA NIH HHS [DA06244] NR 56 TC 33 Z9 33 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD FEB 19 PY 1993 VL 36 IS 4 BP 479 EP 490 DI 10.1021/jm00056a008 PG 12 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA KN149 UT WOS:A1993KN14900008 PM 8386247 ER PT J AU SCHWARTZ, PJ AF SCHWARTZ, PJ TI ELECTROMAGNETIC-FIELDS AND CIRCADIAN-RHYTHMS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP SCHWARTZ, PJ (reprint author), NIMH,BETHESDA,MD 20892, USA. NR 5 TC 3 Z9 3 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 17 PY 1993 VL 269 IS 7 BP 868 EP 868 DI 10.1001/jama.269.7.868 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA KL474 UT WOS:A1993KL47400016 PM 8426444 ER PT J AU FELD, R RUBINSTEIN, L THOMAS, PA AF FELD, R RUBINSTEIN, L THOMAS, PA TI ADJUVANT CHEMOTHERAPY WITH CYCLOPHOSPHAMIDE, DOXORUBICIN, AND CISPLATIN IN PATIENTS WITH COMPLETELY RESECTED STAGE-I NON-SMALL-CELL LUNG-CANCER SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID BRONCHOGENIC-CARCINOMA; RANDOMIZED TRIAL; ADENOCARCINOMA; THERAPY AB Background: Most studies of adjuvant chemotherapy, radiotherapy, or immunotherapy in non-small-cell lung cancer patients with complete surgical resection of disease have shown negative results. However, two studies of stage II and III disease by our Lung Cancer Study Group suggested an advantage to adjuvant therapy with cyclophosphamide, doxorubicin, and cisplatin (CAP). Purpose: Since neither of those studies had an untreated control, the Lung Cancer Study Group undertook a trial that included a control group and also offered the potential benefit of adjuvant therapy with CAP to patients with T1, N1 or T2, N0 (stage I) non-small-cell lung cancer. Methods: After complete resection, eligible patients with stage I disease were classified by known prognostic factors and randomly assigned to receive or not to receive four courses of CAP at 3-week intervals beginning on day 30 after surgery. The CAP regimen consisted of 400 mg/m2 cyclophosphamide, 40 mg/m2 doxorubicin, and 60 mg/m2 Cisplatin. Stratification by prognostic factors was as follows: histology (squamous versus nonsquamous), white blood cell count before surgery (greater-than-or-equal-to 9100/mm3 versus <9100/mm3), and Karnofsky performance status before surgery (less-than-or-equal-to 90% versus 100%). Results: Of the 269 patients entered in the study, 101 had recurrence and 127 have died. Mean time since randomization is 6.4 years; mean follow-up is 3.8 years. There were no differences in time to recurrence or overall survival (not stratified by histology) between the two groups, even when analyses were adjusted for prognostic variables. There was one treatment-related death on the CAP arm due to infection during neutropenia. Only 53% of the eligible patients received all four courses of CAP, and only 57% of such patients received all four cycles on time. In 74% of the patients, the site of initial recurrence was distant. Conclusions: The most likely explanations for the lack of efficacy of CAP are poor compliance to the protocol and relative inactivity of the regimen, compared with the activity of drug combinations used in more recent studies. On the basis of this trial, adjuvant therapy with CAP should not be recommended for patients with resected stage I lung cancer. Implications: Further trials to test adjuvant therapy are indicated, but investigators should use better antiemetics to improve patient compliance as well as more active chemotherapy regimens. C1 UNIV TORONTO,TORONTO M5S 1A1,ONTARIO,CANADA. NCI,BETHESDA,MD 20892. ILLINOIS CANC COUNCIL,CHICAGO,IL. FU NCI NIH HHS [CA-36045] NR 21 TC 129 Z9 134 U1 0 U2 1 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 17 PY 1993 VL 85 IS 4 BP 299 EP 306 DI 10.1093/jnci/85.4.299 PG 8 WC Oncology SC Oncology GA KL585 UT WOS:A1993KL58500009 PM 8381187 ER PT J AU GRIDLEY, G MCLAUGHLIN, JK EKBOM, A KLARESKOG, L ADAMI, HO HACKER, DG HOOVER, R FRAUMENI, JF AF GRIDLEY, G MCLAUGHLIN, JK EKBOM, A KLARESKOG, L ADAMI, HO HACKER, DG HOOVER, R FRAUMENI, JF TI INCIDENCE OF CANCER AMONG PATIENTS WITH RHEUMATOID-ARTHRITIS SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID NONSTEROIDAL ANTIINFLAMMATORY DRUGS; NON-HODGKINS-LYMPHOMA; LARGE-BOWEL CANCER; COLON CARCINOGENESIS; COLORECTAL-CANCER; ASPIRIN USE; FOLLOW-UP; RISK; MORTALITY; EPIDEMIOLOGY AB Background: To evaluate hypotheses about the relationship between immune alterations and cancer, several investigators have determined cancer incidence in groups of patients with rheumatoid arthritis (RA), a chronic autoimmune disease. The primary finding has been an increased risk of hematopoietic cancers. Purpose: In this study, we have attempted to refine estimates of the association between RA and subsequent development of specific cancers. Methods: We investigated site-specific cancer risk associated with RA in a population-based cohort study of 11 683 Swedish men and women with a hospital (inpatient) diagnosis of RA. These case patients were identified from 1965 to 1983 and had follow-up through 1984 by computer linkage of the Swedish Hospital Inpatient Register to the National Swedish Cancer Registry (840 case patients with cancer) and the Swedish Registry of Causes of Death. Cancer risk was estimated by standardized incidence ratios (SIRs) for specific cancers. Results: For men and women overall, there were decreased risks for cancers of the colon (SIR = 0.63; 95% confidence interval [CI] = 0.5-0.9), rectum (SIR = 0.72; 95% CI = 0.5-1.1), and stomach (SIR = 0.63; 95% CI = 0.5-0.9) and an increased risk for lymphomas (SIR = 1.98; 95% CI = 1.5-2.6). Conclusions: The reduced risk for colorectal cancer in patients with RA is consistent with previous studies of RA patients and with reports which state that use of nonsteroidal anti-inflammatory drugs may protect against the development of large bowel cancers. The excess of lymphomas also confirms a number of earlier investigations of RA patients. C1 UNIV HOSP UPPSALA,CANC EPIDEMIOL,UPPSALA,SWEDEN. INFORMAT MANAGEMENT SERV INC,SILVER SPRING,MD. RP GRIDLEY, G (reprint author), NCI,DIV CANC ETIOL,6130 EXECUT BLVD,EPN 443,ROCKVILLE,MD 20852, USA. RI Hacker, David/D-5134-2011; OI Hacker, David/0000-0002-0762-9667 NR 51 TC 489 Z9 505 U1 0 U2 11 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 17 PY 1993 VL 85 IS 4 BP 307 EP 311 DI 10.1093/jnci/85.4.307 PG 5 WC Oncology SC Oncology GA KL585 UT WOS:A1993KL58500010 PM 8426374 ER PT J AU YORK, DM DARDEN, TA PEDERSEN, LG ANDERSON, MW AF YORK, DM DARDEN, TA PEDERSEN, LG ANDERSON, MW TI MOLECULAR-DYNAMICS SIMULATION OF HIV-1 PROTEASE IN A CRYSTALLINE ENVIRONMENT AND IN SOLUTION SO BIOCHEMISTRY LA English DT Article ID HUMAN IMMUNODEFICIENCY VIRUS-1; BINDING FREE-ENERGIES; ASPARTIC PROTEASE; TYPE-1 PROTEASE; NUCLEIC-ACIDS; FORCE-FIELD; INHIBITOR; RESOLUTION; PROTEINS; COMPLEX AB Simulations of the unbound form of the human immunodeficiency virus type 1 protease have been carried out to 200 ps in a crystalline environment and in solution. Solution simulations were performed with and without charge-balancing counterions. The results are compared with the 2.8-angstrom crystallographic structure of Wlodawer et al. [(1989) Science 245, 6161, and a proposed model for the solution structure which involves local refolding of the flap regions is presented. The simulations suggest the crystal packing environment of the protease dimer stabilizes the flaps in an extended conformation. Solvation of the dimer leads to local refolding of the flaps which contract toward the active site, forming increased overlap and stronger intersubunit hydrogn bonding at the tips. The degree to which the flaps overlap in solution is observed to depend on the charge state of the system. C1 UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599. RP YORK, DM (reprint author), NIEHS,MOLEC TOXICOL LAB,RES TRIANGLE PK,NC 27709, USA. RI Pedersen, Lee/E-3405-2013 OI Pedersen, Lee/0000-0003-1262-9861 FU NHLBI NIH HHS [HL27995] NR 51 TC 67 Z9 68 U1 1 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 16 PY 1993 VL 32 IS 6 BP 1443 EP 1453 DI 10.1021/bi00057a007 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM703 UT WOS:A1993KM70300007 PM 8431424 ER PT J AU KLEINER, DE TUUTTILA, A TRYGGVASON, K STETLERSTEVENSON, WG AF KLEINER, DE TUUTTILA, A TRYGGVASON, K STETLERSTEVENSON, WG TI STABILITY ANALYSIS OF LATENT AND ACTIVE 72-KDA TYPE-IV COLLAGENASE - THE ROLE OF TISSUE INHIBITOR OF METALLOPROTEINASES-2 (TIMP-2) SO BIOCHEMISTRY LA English DT Article ID MATRIX METALLOPROTEINASES; INTERSTITIAL COLLAGENASE; ACTIVATION; COMPLEX; PROGELATINASE; FIBRONECTIN; BINDING; IDENTIFICATION; PURIFICATION; FIBROBLASTS AB The degradation of extracellular matrix is an important facet of many physiological and pathological processes. The collagenases form a family of matrix degradative enzymes that have similar active site sequences and activation mechanisms and are inhibited by a specific class of proteinase inhibitors referred to as tissue inhibitors of metalloproteinases. Regulation of enzyme activity is a complex process involving control at multiple levels: message transcription and translation, activation of latent proenzymes, inhibition of activity by specific inhibitors, and degradation of activated enzymes. We have examined the role of the proteinase inhibitor tissue inhibitor of metalloproteinases-2 (TIMP-2) on two of these processes: the autoactivation and autodegradation of the human 72-kDa type IV collagenase. We compared the stability of the enzyme in these two processes using three different enzyme preparations: the enzyme-inhibitor complex as isolated from human A2058 melanoma cells, recombinant enzyme free of TIMP-2, and enzyme separated from TIMP-2 by acid denaturation. We have found little evidence to support the hypothesis that the enzyme is able to autoactivate, as no autoactivation occurs in the presence of TIMP-2 and only 20% autoactivation occurs in its absence, and then only after 24 h of incubation at 37-degrees-C. However, TIMP-2 does appear to inhibit autodegradation, possibly by a mechanism distinct from its ability to inhibit substrate proteolysis. Enzyme isolated via chromatography involving acid mobile phases produces a mixture of cleavage products that is mostly denatured, inactive enzyme fragments. The role of TIMP-2 as an inhibitor of autodegradation suggests that the enzyme may show two physiological phenotypes: the free enzyme having a high level of activity and rapid autodegradation and enzyme-inhibitor complex having a low level of activity resistant to autodegradation. C1 UNIV OULU,BIOCTR,SF-90570 OULU,FINLAND. UNIV OULU,DEPT BIOCHEM,SF-90570 OULU,FINLAND. RP KLEINER, DE (reprint author), NCI,PATHOL LAB,BLDG 10,ROOM 2N212,BETHESDA,MD 20892, USA. RI Stetler-Stevenson, William/H-6956-2012; OI Stetler-Stevenson, William/0000-0002-5500-5808; Kleiner, David/0000-0003-3442-4453 NR 35 TC 94 Z9 97 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 16 PY 1993 VL 32 IS 6 BP 1583 EP 1592 DI 10.1021/bi00057a024 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM703 UT WOS:A1993KM70300024 PM 8431437 ER PT J AU INGRAHAM, LJ WENDER, PH AF INGRAHAM, LJ WENDER, PH TI DEPRESSION AND SUBSTANCE-ABUSE MAY HAVE GENETIC LINK SO AMERICAN FAMILY PHYSICIAN LA English DT Editorial Material RP INGRAHAM, LJ (reprint author), NIMH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ACAD FAMILY PHYSICIANS PI KANSAS CITY PA 8880 WARD PARKWAY, KANSAS CITY, MO 64114-2797 SN 0002-838X J9 AM FAM PHYSICIAN JI Am. Fam. Physician PD FEB 15 PY 1993 VL 47 IS 3 BP 648 EP 648 PG 1 WC Primary Health Care; Medicine, General & Internal SC General & Internal Medicine GA KN938 UT WOS:A1993KN93800014 ER PT J AU FERNICOLA, DJ BOODHOO, VR ROBERTS, WC AF FERNICOLA, DJ BOODHOO, VR ROBERTS, WC TI PROLONGED SURVIVAL (74 YEARS) IN UNOPERATED TETRALOGY OF FALLOT WITH ASSOCIATED MITRAL-VALVE PROLAPSE SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article C1 PARISH MED CTR,TITUSVILLE,FL. RP FERNICOLA, DJ (reprint author), NHLBI,PATHOL BRANCH,BETHESDA,MD 20892, USA. NR 18 TC 2 Z9 3 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD FEB 15 PY 1993 VL 71 IS 5 BP 479 EP 483 DI 10.1016/0002-9149(93)90465-O PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA KM552 UT WOS:A1993KM55200031 PM 8430650 ER PT J AU MAUTNER, GC MAUTNER, SL LIN, FR ROGGIN, GM ROBERTS, WC AF MAUTNER, GC MAUTNER, SL LIN, FR ROGGIN, GM ROBERTS, WC TI AMOUNTS OF CORONARY ARTERIAL LUMINAL NARROWING AND COMPOSITION OF THE MATERIAL CAUSING THE NARROWING IN BUERGERS-DISEASE SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID ACUTE MYOCARDIAL-INFARCTION; MORPHOMETRIC ANALYSIS; PLAQUES C1 SUBURBAN HOSP,DEPT MED,BETHESDA,MD. RP MAUTNER, GC (reprint author), NHLBI,PATHOL BRANCH,BETHESDA,MD 20892, USA. NR 21 TC 14 Z9 16 U1 0 U2 1 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD FEB 15 PY 1993 VL 71 IS 5 BP 486 EP 490 DI 10.1016/0002-9149(93)90467-Q PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA KM552 UT WOS:A1993KM55200033 PM 8430652 ER PT J AU MUNOZ, A SCHRAGER, LK BACELLAR, H SPEIZER, I VERMUND, SH DETELS, R SAAH, AJ KINGSLEY, LA SEMINARA, D PHAIR, JP AF MUNOZ, A SCHRAGER, LK BACELLAR, H SPEIZER, I VERMUND, SH DETELS, R SAAH, AJ KINGSLEY, LA SEMINARA, D PHAIR, JP TI TRENDS IN THE INCIDENCE OF OUTCOMES DEFINING ACQUIRED-IMMUNODEFICIENCY-SYNDROME (AIDS) IN THE MULTICENTER AIDS COHORT STUDY - 1985-1991 SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE ACQUIRED IMMUNODEFICIENCY SYNDROME; EPIDEMIOLOGIC METHODS; HIV-1; LYMPHOMA; NERVOUS SYSTEM DISEASES; OPPORTUNISTIC INFECTIONS; PNEUMONIA, PNEUMOCYSTIS-CARINII; SARCOMA, KAPOSIS ID PNEUMOCYSTIS-CARINII PNEUMONIA; CONTROLLED TRIAL; HIV INFECTION; ZIDOVUDINE; PROPHYLAXIS; EFFICACY; LYMPHOMA; SAFETY AB Incidence of clinical outcomes defining acquired immunodeficiency syndrome (AIDS) may be expected to change as a consequence of progressive immunosuppression and use of chemoprophylaxis before the onset of AIDS. Using Poisson regression methods, we examined trends in the incidence of initial and secondary AIDS-defining illnesses from 1985 to 1991 among 2,627 homosexual men participating in the Multicenter AIDS Cohort Study who were seropositive for human immunodeficiency virus type 1. The incidence of Pneumocystis carinii pneumonia rose steeply until 1987 but has declined since then (p < 0.001), while the other AIDS-defining conditions have showed significant (p less-than-or-equal-to 0.039) upward trends. Trends for Kaposi's sarcoma, lymphoma, neurologic disease, and cytomegalovirus/herpes simplex virus infections were explained by progressive immunosuppression, but residual downward and upward trends were present for P. carinii pneumonia and other opportunistic infections (bacterial, fungal, and protozoal infections and wasting syndrome). Despite selection bias, those receiving P. carinii pneumonia chemoprophylaxis showed a significantly lower incidence of P. carinii pneumonia (relative risk = 0.32, 95% confidence interval 0.16-0.63), and the time trends of P. carinii pneumonia were explained by progressive immunosuppression and use of prophylaxis. No significant effects on all other diagnoses were seen in those selected to receive antiretroviral therapy. Secondary diagnoses showed a strongly significant (p < 0.001) increase in non-P. carinii pneumonia and non-Kaposi's sarcoma among those with initial diagnoses of Kaposi's sarcoma. Overall, the trend observed in the incidence of other opportunistic infections underscores the need for developing and testing new strategies to curtail or delay the onset of these diseases. C1 NIAID,DIV AIDS,EPIDEMIOL BRANCH,BETHESDA,MD 20892. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,DEPT EPIDEMIOL,LOS ANGELES,CA 90024. UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT INFECT DIS & MICROBIOL,PITTSBURGH,PA 15260. NCI,DIV CANC ETIOL,BETHESDA,MD 20892. NORTHWESTERN UNIV,SCH MED,CHICAGO,IL 60611. RP MUNOZ, A (reprint author), JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT EPIDEMIOL,624 N BROADWAY,ROOM 797,BALTIMORE,MD 21205, USA. OI Vermund, Sten/0000-0001-7289-8698 FU NIAID NIH HHS [AI-32535, AI-72634, AI-72676] NR 19 TC 162 Z9 163 U1 0 U2 2 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD FEB 15 PY 1993 VL 137 IS 4 BP 423 EP 438 PG 16 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KV977 UT WOS:A1993KV97700006 PM 8096356 ER PT J AU KRUTZSCH, HC INMAN, JK AF KRUTZSCH, HC INMAN, JK TI N-ISOPROPYLIODOACETAMIDE IN THE REDUCTION AND ALKYLATION OF PROTEINS - USE IN MICROSEQUENCE ANALYSIS SO ANALYTICAL BIOCHEMISTRY LA English DT Article C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RP KRUTZSCH, HC (reprint author), NCI,PATHOL LAB,BETHESDA,MD 20892, USA. NR 15 TC 38 Z9 38 U1 1 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD FEB 15 PY 1993 VL 209 IS 1 BP 109 EP 116 DI 10.1006/abio.1993.1089 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA KN637 UT WOS:A1993KN63700016 PM 8465942 ER PT J AU DARAWSHE, S RIVAS, G MINTON, AP AF DARAWSHE, S RIVAS, G MINTON, AP TI RAPID AND ACCURATE MICROFRACTIONATION OF THE CONTENTS OF SMALL CENTRIFUGE TUBES - APPLICATION IN THE MEASUREMENT OF MOLECULAR-WEIGHT OF PROTEINS VIA SEDIMENTATION EQUILIBRIUM SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID ANALYTICAL ULTRACENTRIFUGATION RP DARAWSHE, S (reprint author), NIDDKD,BIOCHEM PHARMACOL LAB,BETHESDA,MD 20892, USA. OI Rivas, German/0000-0003-3450-7478 NR 26 TC 34 Z9 34 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD FEB 15 PY 1993 VL 209 IS 1 BP 130 EP 135 DI 10.1006/abio.1993.1092 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA KN637 UT WOS:A1993KN63700019 PM 8465945 ER PT J AU ROONEY, JF STRAUS, SE MANNIX, ML WOHLENBERG, CR ALLING, DW DUMOIS, JA NOTKINS, AL AF ROONEY, JF STRAUS, SE MANNIX, ML WOHLENBERG, CR ALLING, DW DUMOIS, JA NOTKINS, AL TI ORAL ACYCLOVIR TO SUPPRESS FREQUENTLY RECURRENT HERPES LABIALIS - A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE ACYCLOVIR; HERPES LABIALIS; VIRUS REPLICATION; IMMUNOCOMPETENCE ID SIMPLEX VIRUS-INFECTION; GENITAL HERPES; ERYTHEMA MULTIFORME; PREVENTION; REACTIVATION AB Objective: To determine whether oral acyclovir reduces the incidence of recurrent herpes labialis in otherwise healthy patients with proven frequently recurrent disease. Design: Randomized, double-blind, placebo-controlled, crossover trial. Setting: Outpatient facility of the Clinical Center, National Institutes of Health, Bethesda, Maryland. Patients: Fifty-six otherwise healthy adults who reported frequently recurrent herpes labialis (greater-than-or-equal-to 6 episodes/y) were enrolled into the study. During a 4-month observation period, 22 patients had herpes labialis two or more times and were eligible for study treatment. Interventions: Twenty-two patients were randomized to receive either acyclovir, 400 mg twice daily, or matched placebo for 4 months. After the first treatment period, patients were given the alternate treatment for another 4 months and were then taken off study medication to observe the first post-treatment recurrence. Recurrent outbreaks were determined by examination and by viral culture. Results: Twenty patients completed blind treatment with both acyclovir and placebo. The median time to first clinically documented recurrence was 46 days for placebo courses and 118 days for acyclovir courses (P = 0.05). The mean number of recurrences per 4-month treatment period was 1.80 episodes per patient during placebo treatment and 0.85 episodes per patient during acyclovir treatment (P = 0.009). The mean number of virologically confirmed recurrences per patient was 1.40 with placebo therapy compared with 0.40 with acyclovir (P = 0.003). Conclusions: Oral acyclovir, 400 mg twice daily, is effective in suppressing herpes labialis in immunocompetent adults confirmed to have frequently recurrent infection. Treatment with acyclovir in this study resulted in a 53% reduction in the number of clinical recurrences and a 71% reduction in virus culture-positive recurrences compared with placebo therapy. C1 NIAID,BETHESDA,MD 20892. RP ROONEY, JF (reprint author), NIDR,ORAL MED LAB,BLDG 30,ROOM 121,BETHESDA,MD 20892, USA. NR 23 TC 85 Z9 86 U1 0 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD FEB 15 PY 1993 VL 118 IS 4 BP 268 EP 272 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA KL457 UT WOS:A1993KL45700004 PM 8380540 ER PT J AU RAO, PV HORWITZ, J ZIGLER, JS AF RAO, PV HORWITZ, J ZIGLER, JS TI ALPHA-CRYSTALLIN, A MOLECULAR CHAPERONE, FORMS A STABLE COMPLEX WITH CARBONIC-ANHYDRASE UPON HEAT DENATURATION SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID B-CRYSTALLIN; PROTEIN; TISSUES; SUBUNIT C1 UNIV CALIF LOS ANGELES, SCH MED, JULES STEIN EYE INST, LOS ANGELES, CA USA. RP RAO, PV (reprint author), NEI, MECH OCULAR DIS LAB, BETHESDA, MD 20892 USA. NR 30 TC 106 Z9 107 U1 1 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 15 PY 1993 VL 190 IS 3 BP 786 EP 793 DI 10.1006/bbrc.1993.1118 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA KM643 UT WOS:A1993KM64300016 PM 8094957 ER PT J AU TAKAHASHI, H GUROFF, G AF TAKAHASHI, H GUROFF, G TI EVIDENCE FOR AN INDIRECT EFFECT OF NERVE GROWTH-FACTOR (NGF) ON THE ADP-RIBOSYLATION OF A 22 KDA RHO-LIKE PROTEIN IN PC12 CELLS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID GTP-BINDING PROTEINS; DIFFERENTIATION; BOTULINUM; MICROINJECTION; INHIBITION RP TAKAHASHI, H (reprint author), NICHHD,GROWTH FACTORS SECT,BETHESDA,MD 20892, USA. NR 18 TC 5 Z9 5 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD FEB 15 PY 1993 VL 190 IS 3 BP 1156 EP 1162 DI 10.1006/bbrc.1993.1171 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA KM643 UT WOS:A1993KM64300069 PM 8439316 ER PT J AU DUPONT, WD PARL, FF HARTMANN, WH BRINTON, LA WINFIELD, AC WORRELL, JA SCHUYLER, PA PLUMMER, WD AF DUPONT, WD PARL, FF HARTMANN, WH BRINTON, LA WINFIELD, AC WORRELL, JA SCHUYLER, PA PLUMMER, WD TI BREAST-CANCER RISK ASSOCIATED WITH PROLIFERATIVE BREAST DISEASE AND ATYPICAL HYPERPLASIA SO CANCER LA English DT Article DE BREAST CANCER; BREAST DISEASE; PROLIFERATIVE DISEASE; ATYPICAL HYPERPLASIA; FAMILY HISTORY; CYSTS; CALCIFICATIONS ID TERM FOLLOW-UP; LESIONS; WOMEN; EPIDEMIOLOGY AB Background. Women with proliferative breast disease (PD) have been observed to have an increased risk of breast cancer. The authors evaluated the effect of PD on breast cancer risk in a case-control study among participants of the Breast Cancer Detection Demonstration Project (BCDDP). Methods. More than 280,000 women were screened in the BCDDP at 29 centers. Study subjects were selected from BCDDP participants who underwent biopsy that revealed benign breast tissue. There were five BCDDP centers for which histologic slides were available on more than 85% of the benign biopsy specimens. Case patients for this study were the 95 women from these five centers who had breast cancer develop during follow-up. Two matched control patients who did not have breast cancer develop were selected for each case. The biopsy slides were reviewed by two pathologists who were blinded with regard to cancer outcome. Results. Women with atypical hyperplasia (AH) had 4.3 times the breast cancer risk of women without PD (95% confidence interval (CI], 1.7-11). In women with PD lacking AH, the relative risk was 1.3 (95% CI, 0.77-2.2). A family history of breast cancer (FH) increased breast cancer risk 2.4 times (95% Cl, 1.4-4.3). The joint occurrence of FH and AH had a strong synergistic effect on breast cancer risk. Conclusions. AH is a reliable marker of increased breast cancer risk among women undergoing breast biopsy. C1 MEM MED CTR,DEPT PATHOL,LONG BEACH,CA. VANDERBILT UNIV,MED CTR,SCH MED,DEPT PATHOL,NASHVILLE,TN 37232. VANDERBILT UNIV,MED CTR,SCH MED,DEPT RADIOL,NASHVILLE,TN 37232. NCI,ENVIRONM EPIDEMIOL BRANCH,BETHESDA,MD 20892. RP DUPONT, WD (reprint author), VANDERBILT UNIV,MED CTR,SCH MED,DEPT PREVENT MED,A-1124 MED CTR N,NASHVILLE,TN 37232, USA. RI Dupont, William/I-4430-2012; Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 FU NCI NIH HHS [R01 CA46492, R01 CA50468] NR 25 TC 313 Z9 330 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 15 PY 1993 VL 71 IS 4 BP 1258 EP 1265 DI 10.1002/1097-0142(19930215)71:4<1258::AID-CNCR2820710415>3.0.CO;2-I PG 8 WC Oncology SC Oncology GA KN064 UT WOS:A1993KN06400014 PM 8435803 ER PT J AU AZNAVOORIAN, S MURPHY, AN STETLERSTEVENSON, WG LIOTTA, LA AF AZNAVOORIAN, S MURPHY, AN STETLERSTEVENSON, WG LIOTTA, LA TI MOLECULAR ASPECTS OF TUMOR-CELL INVASION AND METASTASIS SO CANCER LA English DT Review ID BASEMENT-MEMBRANE COLLAGEN; HUMAN-MELANOMA CELLS; BRONCHIAL EPITHELIAL-CELLS; MATRIX-MEDIATED MOTILITY; HUMAN AMNIOTIC MEMBRANE; GROWTH-FACTOR-BETA; NIH 3T3 CELLS; IV COLLAGENASE; TISSUE INHIBITOR; FIBRONECTIN RECEPTOR RP AZNAVOORIAN, S (reprint author), NCI,PATHOL LAB,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 183 TC 422 Z9 484 U1 1 U2 13 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 15 PY 1993 VL 71 IS 4 BP 1368 EP 1383 DI 10.1002/1097-0142(19930215)71:4<1368::AID-CNCR2820710432>3.0.CO;2-L PG 16 WC Oncology SC Oncology GA KN064 UT WOS:A1993KN06400031 PM 8435813 ER PT J AU LIVOLSI, VA CLAUSEN, KP GRIZZLE, W NEWTON, W PRETLOW, TG AAMODT, R AF LIVOLSI, VA CLAUSEN, KP GRIZZLE, W NEWTON, W PRETLOW, TG AAMODT, R TI THE COOPERATIVE HUMAN TISSUE NETWORK - AN UPDATE SO CANCER LA English DT Note DE COOPERATIVE HUMAN TISSUE NETWORK; TISSUE PROCUREMENT; HUMAN TISSUE RESEARCH AB Background. During the past decade, the National Cancer Institute became aware of a lack of availability of human tissues for research, especially in the fields of molecular biology, genetics, and immunology. Methods. In 1987, the National Cancer Institute (NCI) established the Cooperative Human Tissue Network (CHTN) by funding three institutions that had extensive experience in the procurement and distribution of tissues for research. Results. Since its inception, the CHTN has been expanded to five member institutions, with the addition of the Columbus Children's Hospital (the Pediatric Division for the procurement of pediatric tissues) and Case Western Reserve University. Each of the five divisions have established regional networks of institutions that supply tissues to the major divisions. From 1987 through 1991, the CHTN has distributed more than 29,000 human tissue samples and supplied approximately 500 researchers throughout the country. Conclusions. This report describes the development and current status of the CHTN, a valuable resource to the research community. C1 OHIO STATE UNIV,DEPT PATHOL,COLUMBUS,OH 43210. UNIV ALABAMA,MED CTR,DEPT PATHOL,BIRMINGHAM,AL 35294. COLUMBUS CHILDRENS HOSP,DEPT ONCOL,COLUMBUS,OH. COLUMBUS CHILDRENS HOSP,DEPT PATHOL,COLUMBUS,OH. CASE WESTERN RESERVE UNIV,SCH MED,DEPT PATHOL,CLEVELAND,OH 44106. NCI,BETHESDA,MD 20892. RP LIVOLSI, VA (reprint author), UNIV PENN,MED CTR,DEPT PATHOL & LAB MED,3400 SPRUCE ST,FOUNDERS 6-042,PHILADELPHIA,PA 19104, USA. NR 4 TC 35 Z9 37 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 15 PY 1993 VL 71 IS 4 BP 1391 EP 1394 DI 10.1002/1097-0142(19930215)71:4<1391::AID-CNCR2820710434>3.0.CO;2-X PG 4 WC Oncology SC Oncology GA KN064 UT WOS:A1993KN06400033 PM 8435815 ER PT J AU BLANEY, SM COLE, DE BALIS, FM GODWIN, K POPLACK, DG AF BLANEY, SM COLE, DE BALIS, FM GODWIN, K POPLACK, DG TI PLASMA AND CEREBROSPINAL-FLUID PHARMACOKINETIC STUDY OF TOPOTECAN IN NONHUMAN-PRIMATES SO CANCER RESEARCH LA English DT Note ID DRUGS; MODEL AB Topotecan, a water soluble semisynthetic analogue of camptothecin, is a topoisomerase I inhibitor that has recently entered phase II clinical trials. Topotecan has shown significant preclinical activity in refractory murine tumors and in human tumor xenograft models. In addition, objective antineoplastic activity has been observed in recent adult phase I clinical trials. Topotecan is unstable in solution and is rapidly and spontaneously converted to a less active open ring form which predominates at physiological pH. This study was undertaken to better define the pharmacokinetic behavior of this highly unstable compound in both plasma and cerebrospinal fluid (CSF) and to measure the degree of CSF penetration of this novel antineoplastic agent. Three nonhuman primates with indwelling Ommaya reservoirs received 10 mg/m2 i.v. topotecan administered as a 10-min infusion. Frequent plasma and CSF samples were obtained and immediately extracted and assayed with a reverse phase high performance liquid chromatography assay to quantitate the concentration of topotecan lactone). Samples were then acidified and reinjected to quantitate total drug (lactone ring plus open ring). Peak plasma concentrations of topotecan ranged from 0.27 to 0.45 muM. Plasma disappearance of the lactone ring was biexponential with a distribution half-life (t1/2alpha) of 22 +/- 5 min and an elimination half-life (t1/2beta) Of 1.3 +/- 0.1 h. Total body clearance of topotecan was 72.1 +/- 15.8 liters/h/m2. The volume of distribution at steady state was 88.6 +/- 33.2 liter/m2. Peak CSF concentrations of topotecan occurred at 30 min following drug administration and ranged from 0.044 to 0.074 muM. CSF disappearance paralleled that in plasma. The mean ratio of the area under the CSF concentration-time curve to that in plasma was 0.32 (range, 0.29 to 0.37). The mean CSF penetration of topotecan exceeds 30%, which is significantly greater than the penetration of most structurally similar chemotherapeutic agents. The impact of chemotherapy on the survival of patients with primary or metastatic central nervous system malignancies is very limited. Therefore, this novel antineoplastic agent is an excellent candidate for further study in patients with high risk or refractory central nervous system tumors. C1 WALTER REED ARMY MED CTR,WASHINGTON,DC 20307. RP BLANEY, SM (reprint author), NCI,PEDIAT BRANCH,BETHESDA,MD 20892, USA. NR 22 TC 80 Z9 81 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 725 EP 727 PG 3 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300005 PM 8428353 ER PT J AU GOTTESMAN, MM AF GOTTESMAN, MM TI HOW CANCER-CELLS EVADE CHEMOTHERAPY - 16TH RICHARD-AND-HINDA-ROSENTHAL-FOUNDATION AWARD LECTURE SO CANCER RESEARCH LA English DT Article ID MULTIDRUG-RESISTANCE GENE; BACTERIAL TRANSPORT PROTEINS; KB CARCINOMA-CELLS; P-GLYCOPROTEIN GENE; COMPLEMENTARY-DNA; DRUG-RESISTANCE; TRANSGENIC MICE; MDR1 GENE; INTERNAL DUPLICATION; BONE-MARROW RP GOTTESMAN, MM (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. NR 73 TC 374 Z9 386 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 747 EP 754 PG 8 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300011 PM 8094031 ER PT J AU ZHENG, W BLOT, WJ DIAMOND, EL NORKUS, EP SPATE, V MORRIS, JS COMSTOCK, GW AF ZHENG, W BLOT, WJ DIAMOND, EL NORKUS, EP SPATE, V MORRIS, JS COMSTOCK, GW TI SERUM MICRONUTRIENTS AND THE SUBSEQUENT RISK OF ORAL AND PHARYNGEAL CANCER SO CANCER RESEARCH LA English DT Article ID SQUAMOUS-CELL CARCINOMA; VITAMIN-A; BETA-CAROTENE; SELENIUM; LEUKOPLAKIA; TOCOPHEROL; VEGETABLES; REMISSION; RETINOL; PLASMA AB To investigate the relationship between serum micronutrients and the subsequent risk of oral and pharyngeal cancer, a nested case-control study was conducted within a cohort of 25,802 adults in Washington County, MD, whose blood samples were collected in 1974 and stored at -70-degrees-C for subsequent assays. The serum levels of nutrients in 28 individuals who developed oral and pharyngeal cancer during 1975 to 1990 were compared with levels in 112 matched controls. Serum levels of all individual carotenoids, particularly beta-carotene, were lower among subjects who developed oral and pharyngeal cancer. The risks of this malignancy decreased substantially with increasing serum level of each individual carotenoid. Persons in the highest tertile of total carotenoids had about one-third the cancer risk as those in the lowest tertile. High serum levels of alpha-tocopherol also were related to a low oral cancer risk in later years, but the risks were elevated significantly with increasing serum levels of gamma-tocopherol and selenium. The findings from this study are consistent with many previous epidemiological investigations of dietary factors for oral and pharyngeal cancer and provide further evidence for the potential role of carotenoids and alpha-tocopherol in the chemoprevention of these malignancies. C1 NCI,BIOSTAT BRANCH,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT EPIDEMIOL,BALTIMORE,MD 21218. OUR LADY MERCY MED CTR,BRONX,NY 10466. UNIV MISSOURI,RES REACTOR FACIL,COLUMBIA,MO 65211. FU NCI NIH HHS [CA 36390]; NHLBI NIH HHS [HL 21670] NR 36 TC 92 Z9 92 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 795 EP 798 PG 4 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300019 PM 8428360 ER PT J AU KREITMAN, RJ HANSEN, HJ JONES, AL FITZGERALD, DJP GOLDENBERG, DM PASTAN, I AF KREITMAN, RJ HANSEN, HJ JONES, AL FITZGERALD, DJP GOLDENBERG, DM PASTAN, I TI PSEUDOMONAS EXOTOXIN-BASED IMMUNOTOXINS CONTAINING THE ANTIBODY LL2 OR LL2-FAB' INDUCE REGRESSION OF SUBCUTANEOUS HUMAN B-CELL LYMPHOMA IN MICE SO CANCER RESEARCH LA English DT Article ID CHAIN-CONTAINING IMMUNOTOXINS; MONOCLONAL-ANTIBODIES; ANTITUMOR-ACTIVITY; INVIVO THERAPY; TUMOR-CELLS; RICIN; TOXIN; REAGENTS; DOMAINS AB We have produced immunotoxins using LL2, a monoclonal antibody which binds to human B-cell lymphomas and which, in a radioiodinated form, induced responses in lymphoma patients (D. M. Goldenberg et al., J. Clin. Oncol., 9: 548-564,1991). We have coupled LL2 to Lys-PE38KDEL, a derivative of Pseudomonas exotoxin (PE) which does not bind to the PE receptor. LL2-PE38KDEL was cytotoxic toward several Burkitt's lymphoma lines, with 50% inhibitory concentration values ranging from 2 to 6 ng/ml (10-30 pM). Another immunotoxin, LL2-Fab'-PE38KDEL, was produced by chemically coupling the Fab' fragment of LL2 to Lys-PE38KDEL. LL2-Fab'-PE38KDEL also was cytotoxic toward the Burkitt's cells, with a 50% inhibitory concentration of 1-2 ng/ml (13-24 PM). The antibody LL2 alone had no cytotoxicity toward the malignant cells, and excess LL2 prevented the cytotoxicity of LL2-PE38KDEL and LL2-Fab'-PE38KDEL. Control immunotoxins UPC-10-PE38KDEL and Mu-9-Fab'-PE38KDEL were not cytotoxic. LL2-PE38KDEL and LL2-Fab'-PE38KDEL bound to cells with 50% and 17% of the affinity of LL2, respectively. Both immunotoxins, but not UPC-10-PE38KDEL, prevented the development of CA-46 tumors in nude mice. LL2-PE38KDEL and LL2-Fab'-PE38KDEL, but not the control immunotoxins, led to complete regressions of measurable s.c. CA-46 tumors in nude mice, when given at 50% and 35% of the 50% lethal dose, respectively. LL2 alone significantly retarded the growth of CA-46 tumors but did not cause complete tumor regressions. Immunotoxins containing derivatives of Pseudomonas exotoxin can be targeted to human B-cell lymphoma and merit further study as potential therapeutic agents. C1 NCI,DIV CANC BIOL DIAGNOSIS & CENTERS,MOLEC BIOL LAB,BETHESDA,MD 20892. IMMUNOMED INC,NEWARK,NJ 07103. CTR MOLEC MED & IMMUNOL,GARDEN STATE CANC CTR,NEWARK,NJ 07103. FU NCI NIH HHS [CA39841] NR 30 TC 67 Z9 67 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 819 EP 825 PG 7 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300023 PM 8428363 ER PT J AU LATIF, F DUH, FM GNARRA, J TORY, K KUZMIN, I YAO, M STACKHOUSE, T MODI, W GEIL, L SCHMIDT, L LI, H ORCUTT, ML MAHER, E RICHARDS, F PHIPPS, M FERGUSONSMITH, M LEPASLIER, D LINEHAN, WM ZBAR, B LERMAN, MI AF LATIF, F DUH, FM GNARRA, J TORY, K KUZMIN, I YAO, M STACKHOUSE, T MODI, W GEIL, L SCHMIDT, L LI, H ORCUTT, ML MAHER, E RICHARDS, F PHIPPS, M FERGUSONSMITH, M LEPASLIER, D LINEHAN, WM ZBAR, B LERMAN, MI TI VONHIPPEL-LINDAU SYNDROME - CLONING AND IDENTIFICATION OF THE PLASMA-MEMBRANE CA++-TRANSPORTING ATPASE ISOFORM-2 GENE THAT RESIDES IN THE VONHIPPEL-LINDAU GENE REGION SO CANCER RESEARCH LA English DT Article ID RAT-LIVER NUCLEI; CA-2+-TRANSPORTING ATPASE; MOLECULAR-CLONING; DISEASE; DNA; CHROMOSOME-3P; ONCOGENES; INCREASES; RECEPTOR; CANCER AB We have isolated and analyzed full-length complementary DNA clones encoded by a 200-kilobase gene encompassing the D3S601 locus that resides in the von Hippel-Lindau (VHL) gene region. The deduced amino acid sequence shows 99% identity with the published sequence of the rat plasma membrane Ca++-transporting ATPase isoform 2 complemetary DNA, implying that we have cloned and positioned the human plasma membrane Ca++-transporting ATPase isoform 2 gene within the VHL critical region. The gene is expressed in VHL target tissues and should be considered a potential candidate gene for the VHL disease. C1 NCI,FREDERICK CANC RES & DEV CTR,IMMUNOBIOL LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21702. NCI,SURG BRANCH,BETHESDA,MD 20892. UNIV CAMBRIDGE,DEPT PATHOL,CAMBRIDGE CB2 1QP,ENGLAND. CTR ETUD POLYMORPHISME HUMAIN,F-75010 PARIS,FRANCE. RI MAHER, EAMONN/A-9507-2008 OI MAHER, EAMONN/0000-0002-6226-6918 FU NCI NIH HHS [N01-CO-74102] NR 47 TC 58 Z9 63 U1 0 U2 8 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 861 EP 867 PG 7 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300030 PM 8428366 ER PT J AU YOKOZAKI, H BUDILLON, A TORTORA, G MEISSNER, S BEAUCAGE, SL MIKI, K CHOCHUNG, YS AF YOKOZAKI, H BUDILLON, A TORTORA, G MEISSNER, S BEAUCAGE, SL MIKI, K CHOCHUNG, YS TI AN ANTISENSE OLIGODEOXYNUCLEOTIDE THAT DEPLETES RI-ALPHA SUBUNIT OF CYCLIC AMP-DEPENDENT PROTEIN-KINASE INDUCES GROWTH-INHIBITION IN HUMAN CANCER-CELLS SO CANCER RESEARCH LA English DT Article ID I REGULATORY SUBUNIT; AMINO-ACID SEQUENCE; MOLECULAR-CLONING; RECEPTOR PROTEINS; GENE-EXPRESSION; HL-60 LEUKEMIA; MESSENGER-RNA; HUMAN TESTIS; DIFFERENTIATION; CDNA AB Enhanced expression of the RI(alpha) subunit of cyclic AMP-dependent protein kinase type I has been correlated with cancer cell growth. We provide evidence that RI(alpha) is a growth-inducing protein that may be essential for neoplastic cell growth. Human colon, breast, and gastric carcinoma and neuroblastoma cell lines exposed to a 21-mer human RI(alpha) antisense phosphorothioate oligodeoxynucleotide (S-oligodeoxynucleotide) exhibited growth inhibition with no sign of cytotoxicity. Mismatched sequence (random) S-oligodeoxynucleotides of the same length exhibited no effect. The growth inhibitory effect of RI(alpha) antisense oligomer correlated with a decrease in the RI(alpha) mRNA and protein levels and with an increase in RII(beta) (the regulatory subunit of protein kinase type II) expression. The growth inhibition was abolished, however, when cells were exposed simultaneously to both RI(alpha) and RII(beta) antisense S-oligodeoxynucleotides. The RII(beta) antisense S-oligodeoxynucleotide alone, exhibiting suppression of RII(beta) along with enhancement of RI(alpha) expression, led to slight stimulation of cell growth. These results demonstrate that two isoforms of cyclic AMP receptor proteins, RI(alpha) and RII(beta), are reciprocally related in the growth control of cancer cells and that the RI(alpha) antisense oligodeoxynucleotide, which efficiently depletes the growth stimulatory RI(alpha), is a powerful biological tool toward suppression of malignancy. C1 NCI,CELLULAR IMMUNOL SECT,BLDG 10,ROOM 5B38,BETHESDA,MD 20892. NCI,EXPTL ONCOL SECT,BETHESDA,MD 20892. NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892. US FDA,BETHESDA,MD 20014. TERUMO CO LTD,KANAGAWA,JAPAN. OI Budillon, Alfredo/0000-0002-6330-6053; Yokozaki, Hiroshi/0000-0001-5276-3331 NR 33 TC 87 Z9 88 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 868 EP 872 PG 5 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300031 PM 8428367 ER PT J AU CHERIAN, MG HUANG, PC KLAASSEN, CD LIU, YP LONGFELLOW, DG WAALKES, MP AF CHERIAN, MG HUANG, PC KLAASSEN, CD LIU, YP LONGFELLOW, DG WAALKES, MP TI NATIONAL-CANCER-INSTITUTE WORKSHOP ON THE POSSIBLE ROLES OF METALLOTHIONEIN IN CARCINOGENESIS SO CANCER RESEARCH LA English DT Editorial Material C1 JOHNS HOPKINS UNIV,DEPT BIOCHEM,BALTIMORE,MD 21205. UNIV KANSAS,DEPT PHARMACOL & TOXICOL,KANSAS CITY,KS 66160. NCI,CHEM & PHYS CARCINOGENESIS BRANCH,ROCKVILLE,MD 20892. NCI,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. RP CHERIAN, MG (reprint author), UNIV WESTERN ONTARIO,DEPT PATHOL,LONDON N6A 5C1,ONTARIO,CANADA. NR 0 TC 82 Z9 83 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 922 EP 925 PG 4 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300041 PM 8428370 ER PT J AU MELINO, G KNIGHT, RA THIELE, CJ AF MELINO, G KNIGHT, RA THIELE, CJ TI NEW INSIGHT ON THE BIOLOGY OF NEUROECTODERMAL TUMORS - WORKSHOP REPORT FROM THE UNIVERSITY-OF-ROME-TOR-VERGATA AND THE IDI-IRCCS ON THE GENETICS AND CONTROL OF GROWTH, DIFFERENTIATION, AND PROGRAMMED CELL-DEATH SO CANCER RESEARCH LA English DT Editorial Material C1 NATL HEART & LUNG INST,DEPT CYST FIBROSIS,LONDON,ENGLAND. NCI,PEDIAT BRANCH,BETHESDA,MD 20892. RP MELINO, G (reprint author), UNIV ROME TOR VERGATA,DEPT EXPTL MED,I-00173 ROME,ITALY. NR 0 TC 11 Z9 11 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 15 PY 1993 VL 53 IS 4 BP 926 EP 928 PG 3 WC Oncology SC Oncology GA KL963 UT WOS:A1993KL96300042 PM 8428371 ER PT J AU WISNIEWSKI, J MALEZEWSKI, M KRAWCZYK, Z GEDAMU, L AF WISNIEWSKI, J MALEZEWSKI, M KRAWCZYK, Z GEDAMU, L TI AN UPSTREAM REGION OF THE RAT SPERMATOGENESIS-SPECIFIC HEAT-SHOCK-LIKE HST70 GENE CONFERS TESTIS-SPECIFIC EXPRESSION IN TRANSGENIC MICE SO EUROPEAN JOURNAL OF BIOCHEMISTRY LA English DT Article ID HSP70-RELATED GENE; SEQUENCE-ANALYSIS; PROTEIN; CELLS; CLONING; FAMILY; RNA; MYC AB In order to study the temporal and spatial regulation of a rat testis-specific heat-shock-like hst70 gene, an 0.8-kb fragment of its upstream DNA was fused to the lacZ gene and microinjected into one-cell murine embryos. Independent tgHST1 and tgHST2 transgenic mice strains were established, containing about 5 - 7 and 40 - 60 transgene copies/haploid genome, respectively. Enzyme assays in various tissues showed that transgene-encoded beta-galactosidase accumulates exclusively in testes of transgenic animals and cannot be detected until 16 - 17 days after birth. In-situ assays revealed that the enzyme accumulates mainly in pachytene primary spermatocytes. Our data complement previous studies on the endogenous rat hst70 and suggest that its 0.8-kb upstream region contains sufficient information to function as an active spermatogenesis-specific promoter. C1 UNIV CALGARY,DEPT BIOL SCI,2500 UNIV DR NW,CALGARY T2N 1N4,ALBERTA,CANADA. UNIV WARSAW,INST ZOOL,DEPT EMBRYOL,PL-00325 WARSAW,POLAND. INST ONCOL,DEPT TUMOR BIOL,PL-44100 GLIWICE,POLAND. NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 29 TC 22 Z9 22 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-2956 J9 EUR J BIOCHEM JI Eur. J. Biochem. PD FEB 15 PY 1993 VL 212 IS 1 BP 137 EP 143 DI 10.1111/j.1432-1033.1993.tb17643.x PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KN862 UT WOS:A1993KN86200017 PM 8444152 ER PT J AU ANGULO, JA WATANABE, Y CADET, J LEDOUX, M MCEWEN, BS AF ANGULO, JA WATANABE, Y CADET, J LEDOUX, M MCEWEN, BS TI UP-REGULATION OF FOREBRAIN PROENKEPHALIN MESSENGER-RNA SUBSEQUENT TO NMDA RECEPTOR BLOCKADE SO EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION LA English DT Note DE NMDA RECEPTORS; GLUTAMATE; PROENKEPHALIN MESSENGER RNA; STRIATUM; ACCUMBENS; MK 801 AB Blockade of NMDA (N-methyl-D-aspartate) receptors for 5 h with MK 801 resulted in elevated levels of proenkephalin mRNA (up to 40% above vehicle-injected controls) in medial and anterior aspects of the caudate-putamen (mCPU and aCPU) and the nucleus accumbens (NAc). Increases were dose-dependent, with maximal effect at 0.1 mg/kg (body weight). These results suggest that NMDA receptor activity exerts tonic inhibition on proenkephalin mRNA expression in the striatum and accumbens of the rat brain. C1 NIDA,BETHESDA,MD. RP ANGULO, JA (reprint author), ROCKEFELLER UNIV,NEUROENDOCRINOL LAB,1230 YORK AVE,NEW YORK,NY 10021, USA. NR 5 TC 20 Z9 20 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0922-4106 J9 EUR J PHARM-MOLEC PH JI Eur. J. Pharmacol.-Molec. Pharmacol. Sect. PD FEB 15 PY 1993 VL 244 IS 3 BP 317 EP 318 DI 10.1016/0922-4106(93)90158-6 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KN106 UT WOS:A1993KN10600014 PM 8458404 ER PT J AU DELANEY, TF SINDELAR, WF TOCHNER, Z SMITH, PD FRIAUF, WS THOMAS, G DACHOWSKI, L COLE, JW STEINBERG, SM GLATSTEIN, E AF DELANEY, TF SINDELAR, WF TOCHNER, Z SMITH, PD FRIAUF, WS THOMAS, G DACHOWSKI, L COLE, JW STEINBERG, SM GLATSTEIN, E TI PHASE-I STUDY OF DEBULKING SURGERY AND PHOTODYNAMIC THERAPY FOR DISSEMINATED INTRAPERITONEAL TUMORS SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article DE HEMATOPORPHYRIN DERIVATIVE; PHOTOSENSITIZER; LASER; OVARIAN CANCER; SARCOMA; ABDOMINAL MALIGNANCY ID TRANSITIONAL-CELL-CARCINOMA; SOFT-TISSUE SARCOMAS; PHOTORADIATION THERAPY; BRONCHOSCOPIC PHOTOTHERAPY; HEMATOPORPHYRIN; LIGHT; CHEMOTHERAPY; NEOPLASMS; BLADDER; SPECTRA AB Phase I study designed to determine the maximum tolerated dose of intraoperative photodynamic therapy (PDT) at laparotomy/debulking surgery in patients with refractory or recurrent, disseminated intraperitoneal tumors. Methods and Materials: Patients received dihematoporphyrin ethers (DHE) 1.5-2.5 mg/kg by i.v. injection prior to surgery. Patients resected to less-than-or-equal-to 5 mm of residual disease underwent laser light delivery to all peritoneal surfaces. Results: Fifty-four patients entered the study. Thirty-nine underwent resection and light delivery/PDT. PDT dose was escalated by increasing DHE from 1.5 to 2.5 mg/kg, shortening the interval between DHE injection and surgery from 72 to 48 hr, and increasing the light dose. Initially, 630 nm red light alone was used. In this group, PDT of 2.8-3.0 J/cm2 induced small bowel edema and resulted in 3 small bowel perforations after bowel resection or enterotomy. Further light dose escalation, however, was achieved by switching to less penetrating 514 nm green light to the bowel/mesentery. In later patients, whole peritoneal PDT was supplemented with boost doses of 10-15 J/cm2 red light or 5-7.5 J/cm2 green light to high risk areas. Small bowel complications were not seen after switching to less penetrating green light. Dose limiting toxicities occurred in 2 of 3 patients at the highest light dose of 5.0 j/cm2 green light with boost. These patients had pleural effusions that required thoracentesis and postoperative respiratory support for 7-9 days, while one had a gastric perforation. At potential follow-up times of 3.8-43.1 months (median 22.1 months), 30/39 patients are alive and 9/39 are free of disease. Conclusion: The maximum tolerated dose of intraoperative PDT following debulking surgery performed 48 hr after intravenous administration 2.5 mg/kg DHE is 3.75 J/cm2 of 514 nm green light to the entire peritoneal surface with boosts to 5.0-7.5 J/cm2 of 514 nm green light or 10-15 J/cm2 of 630 nm red light to sites of gross disease encountered at surgery. C1 NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NCI,SURG BRANCH,BETHESDA,MD 20892. NCI,BIOSTAT & DATA MANAGEMENT SECT,BETHESDA,MD 20892. NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 39 TC 103 Z9 113 U1 3 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD FEB 15 PY 1993 VL 25 IS 3 BP 445 EP 457 PG 13 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA KP477 UT WOS:A1993KP47700009 PM 8436523 ER PT J AU OTSU, K KINSELLA, JL HELLER, P FROEHLICH, JP AF OTSU, K KINSELLA, JL HELLER, P FROEHLICH, JP TI SODIUM DEPENDENCE OF THE NA+-H+ EXCHANGER IN THE PRE-STEADY STATE - IMPLICATIONS FOR THE EXCHANGE MECHANISM SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MICROVILLUS MEMBRANE-VESICLES; THYMIC LYMPHOCYTES; MODIFIER SITE; AMILORIDE; ANTIPORT; TRANSPORT; INHIBITION; KINETICS; BINDING; KIDNEY AB The pre-steady state time course of amiloride-sensitive Na+o uptake by the Na+-H+ exchanger in renal brush border membrane vesicles (BBMV) exhibits a burst phase at 0-degrees-C which corresponds to the initial turnover of the exchanger (Otsu, K., Kinsella, J. L., Sacktor, B. S., and Froehlich, J. P. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 4818-4822). Investigation of the Na+o dependence of the Na+-H+ exchanger between 1 and 10 mM Na+ revealed that activation of the burst phase involves at least two Na+ transport sites interacting with positive cooperativity. In this study, characterization of the Na+ transport sites contributing to the burst phase was extended to include Na+ concentrations below 1 mM. Between 0.1 and 1 mM Na+ the amplitude of the burst phase in acid-loaded BBMV (pH(i) 5.7; pH(o) 7.7) exhibited a sigmoidal dependence on [Na+]o, consistent with the presence of a second class of high affinity Na+ transport sites with cooperative binding characteristics. In contrast, steady state Na+ uptake obeyed Michaelis-Menten kinetics, similar to the behavior observed previously at higher (1-10 mm) Na+o concentrations. Treatment of the vesicles with carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone, which induced the formation of an inside-negative membrane potential, increased the burst amplitude but had no effect on the steady state uptake velocity. Experiments performed with alkaline-loaded BBMV (pH(i) 7.7; pH(o) 7.7), which permit only a single turnover of the exchanger, gave a simple hyperbolic dependence of the burst amplitude on [Na+]o (0.5-5 mM). We propose that the change in multiplicity of Na+ transport sites and membrane potential sensitivity that occurs in the transition between the pre-steady state and the steady state of Na+ uptake in acid-loaded vesicles reflects the presence of an oligomer which operates according to a ''flip-flop'' mechanism. The minimum subunit composition inferred from the biphasic [Na+]o dependence of the burst amplitude is a dimer at low (<1 mM) Na+o levels and a tetramer at high [Na+]o. Communication between the subunits producing the complex [Na+]o dependence is controlled by the intravesicular (cytoplasmic) H+ modifier site. Under alkaline conditions (pH 7.7), where this site is unoccupied, the subunits behave as independent units and cease operation after the first turnover. Occupation of the H+ modifier site activates a conformational interaction between the subunits that leads to cooperative Na+o binding, alternation of the transport sites, and repetitive cycling of the Na+-H+ exchanger. C1 NIA,CARDIOVASC SCI LAB,BALTIMORE,MD 21224. NR 36 TC 27 Z9 27 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3184 EP 3193 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100028 PM 8381420 ER PT J AU ARORA, N LEPPLA, SH AF ARORA, N LEPPLA, SH TI RESIDUES-1-254 OF ANTHRAX TOXIN LETHAL FACTOR ARE SUFFICIENT TO CAUSE CELLULAR UPTAKE OF FUSED POLYPEPTIDES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BACILLUS-ANTHRACIS; PROTECTIVE ANTIGEN; ADENYLATE-CYCLASE; ESCHERICHIA-COLI; PSEUDOMONAS EXOTOXIN; LIVING CELLS; TERMINAL END; EXPRESSION; PROTEINS; PURIFICATION AB Anthrax lethal toxin is a complex of protective antigen (PA, 735 amino acids) and lethal factor (LF, 776 amino acids) that lyses certain eukaryotic cells. LF interacts with PA to gain access to the cytosol to assert its toxicity. The internalization of LF requires that PA bind to a specific membrane receptor and be cleaved by a cell-surface protease (probably furin), so as to expose a site on PA to which LF binds with high affinity. To localize LF functional domains, amino, carboxyl, and internal deletions of LF were made. Toxicity was eliminated by deletion of 40 and 47 residues from the amino and carboxyl termini, respectively. Similarly, deleting the first of the four imperfect repeats of 19 amino acids located at residues 308-383 made LF non-toxic, showing that this region is also essential for activity. To identify the minimum region of LF which is required for binding to PA, varying amino-terminal portions of LF were fused to the ADP-ribosylation domain of Pseudomonas exotoxin A. Fusion proteins containing residues 1-254 of LF were toxic when administered with PA, while those having only residues 1-198 of LF were inactive, showing that the PA-binding domain of LF lies within residues 1-254. C1 NIDR, MICROBIAL ECOL LAB, BLDG 30, BETHESDA, MD 20892 USA. NR 50 TC 127 Z9 134 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3334 EP 3341 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100050 PM 8429009 ER PT J AU SZWEDA, LI UCHIDA, K TSAI, L STADTMAN, ER AF SZWEDA, LI UCHIDA, K TSAI, L STADTMAN, ER TI INACTIVATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE BY 4-HYDROXY-2-NONENAL - SELECTIVE MODIFICATION OF AN ACTIVE-SITE LYSINE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LIPID-PEROXIDATION PRODUCTS; ASCITES TUMOR-CELLS; LEUCONOSTOC-MESENTEROIDES; LIVER; ALDEHYDES; SEQUENCE; ACIDS AB Incubation of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides with 4-hydroxy-2-nonenal (HNE) results in a pseudo first-order loss of enzyme activity. The pH dependence of the inactivation rate exhibits an inflection around pH 10, and the enzyme is protected from inactivation by glucose 6-phosphate. Loss of enzyme activity corresponds with the formation of one carbonyl function per enzyme subunit and the appearance of a lysine-HNE adduct. The data presented in this paper are consistent with the view that the epsilon-amino group of a lysine residue in the glucose beta-phosphate-binding site reacts with the double bond (C3) of HNE, resulting in the formation of a stable secondary amine derivative and loss of enzyme activity. We have described a mechanism by which HNE may, in part, mediate free radical damage. In addition, a method for the detection of the lysine-HNE adduct is introduced. C1 NHLBI,BIOCHEM LAB,BLDG 3,RM 222,BETHESDA,MD 20892. NR 19 TC 260 Z9 266 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3342 EP 3347 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100051 PM 8429010 ER PT J AU PETERS, B MEREZHINSKAYA, N DIFFLEY, JFX NOGUCHI, CT AF PETERS, B MEREZHINSKAYA, N DIFFLEY, JFX NOGUCHI, CT TI PROTEIN-DNA INTERACTIONS IN THE EPSILON-GLOBIN GENE SILENCER SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ERYTHROID TRANSCRIPTION FACTOR; PORPHOBILINOGEN DEAMINASE GENE; DOMINANT CONTROL REGION; TRANSGENIC MICE; BINDING PROTEIN; MAMMALIAN-CELLS; FINGER PROTEIN; POLYMERASE-II; FACTOR GATA-1; EXPRESSION AB The developmental control of expression of the human epsilon-globin gene appears to be mediated, at least in part, by a transcriptional silencer in the DNA 5' to the cap site of this gene. We have used site-directed mutagenesis and DNA-protein binding assays to define the active motifs of this epsilon-globin silencer. DNase I footprinting of the silencer region with K562 cell nuclear extracts defined a sequence, which we designate as the epsilon-globin silencer motif or epsilonGSM (epsilon -278 to -258 base pairs (bp)) containing a region (epsilon -270 to -258) with 90% homology to the yeast mating type silencer, ABF-1 (autonomous replicating sequence binding factor one) and which also overlaps at (epsilon -269 to -262) with the human YY1 consensus sequence, an element which mediates transcription repression and activation of viral, mouse, and human genes. The DNase I footprint extended 5' in the silencer region to include an inverted repeat of a six-nucleotide motif (epsilon -267 to -278 bp) which shares 5 of 6 bases with the GATA-1 consensus sequence. In gel mobility shift assays, two specific proteins (A and B) in nuclear extracts from erythroleukemia K562 cells bound to the DNase 1-footprinted region. Protein B, associated with epsilon-globin silencer activity in vitro, required an intact epsilonGSM sequence for binding. Mutation of 5 bases within the epsilonGSM in an epsilon-globin promoter-containing fragment extending upstream to 1400 bp in transient transfection assays increased activity by 3.0-fold compared with the native sequence, suggesting that the silencer activity was mediated by the epsilonGSM sequence. We found that protein A could be displaced by a competitor containing the GATA-1 consensus sequence, suggesting that protein A is a GATA-like protein. The region from -267 to -271 within the epsilonEGSM and GATA-1 homology region was important for binding of both proteins A and B. These data suggest that protein binding to the epsilonGSM and GATA motifs mediate the negative effect of the silencer on transcription, possibly via direct competition for binding to this DNA region. Recombinant yeast ABF-1 and human YY1 bound to the epsilonGSM. Mutating three bases (epsilon -259, -262, -264) in the epsilonGSM decreased the binding affinity of protein B and recombinant human YY1 but increased the binding affinity of recombinant yeast ABF-1. Furthermore, competitor containing the YY1 consensus sequence competed for protein B binding, whereas competitor containing a perfect yeast ABF-1 consensus sequence did not. These data strongly suggest that YY1 is a likely candidate for protein B and raises the possibility that YY1 is involved in the developmental regulation of the human beta-like globin gene cluster. C1 NIDDK,CHEM BIOL LAB,BLDG 10,RM 9N307,BETHESDA,MD 20892. IMPERIAL CANC RES FUND,CLARE HALL LABS,POTTERS BAR EN6 3LD,HERTS,ENGLAND. NR 63 TC 70 Z9 70 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3430 EP 3437 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100064 PM 8429019 ER PT J AU GROLLMAN, EF SAJI, M SHIMURA, Y LAU, JTY ASHWELL, G AF GROLLMAN, EF SAJI, M SHIMURA, Y LAU, JTY ASHWELL, G TI THYROTROPIN REGULATION OF SIALIC-ACID EXPRESSION IN RAT-THYROID CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE; COMPLEX CARBOHYDRATE UNITS; TISSUE-SPECIFIC EXPRESSION; GROWTH FACTOR-I; PORCINE THYROGLOBULIN; GENE-EXPRESSION; POTENTIAL ROLE; LOCALIZATION; RESIDUES; RECEPTOR AB The present study, utilizing the hormone-responsive rat thyroid cell line FRTL-5, presents evidence establishing the regulatory role of thyrotropin in modulating mRNA for beta-galactoside alpha2,6-sialyltransferase, the enzyme responsible for the expression of alpha2,6-linked sialic acid. Both the cell surface membrane and the thyroglobulin secreted by cells grown in the presence of this hormone exhibit a marked decrease in the level of alpha2,6-bound sialic acid with little or no change in the number of alpha2,3-sialic acid residues. An additional, and unexpected, sequel is the finding of a coordinated decrease in all of the core monosaccharide constituents of the secreted thyroglobulin. Both of the above phenomenological changes appear to be at some variance with previously described systems wherein thyrotropin was deemed to increase glycosylation. It is anticipated that further resolution of this apparent difference may provide a clearer definition for the role of the carbohydrate moiety in affecting the biological function of thyroglobulin. C1 NEW YORK STATE DEPT HLTH,ROSWELL PK MEM INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263. RP GROLLMAN, EF (reprint author), NIDDKD,BIOCHEM & METAB LAB,BLDG 10,RM 9B12,BETHESDA,MD 20892, USA. RI Saji, Motoyasu/E-4007-2011 FU NIGMS NIH HHS [GM38193] NR 45 TC 27 Z9 27 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3604 EP 3609 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100088 PM 8429037 ER PT J AU FRETTO, LJ SNAPE, AJ TOMLINSON, JE SEROOGY, JJ WOLF, DL LAROCHELLE, WJ GIESE, NA AF FRETTO, LJ SNAPE, AJ TOMLINSON, JE SEROOGY, JJ WOLF, DL LAROCHELLE, WJ GIESE, NA TI MECHANISM OF PLATELET-DERIVED GROWTH-FACTOR (PDGF)-AA, (PDGF)-AB, AND (PDGF)-BB BINDING TO ALPHA-PDGF AND BETA-PDGF RECEPTOR SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SIGNAL TRANSDUCTION; KINASE ACTIVATION; LIGAND-BINDING; CDNA CLONING; B-PROTEIN; A-CHAIN; EXPRESSION; LOCALIZATION; DIMERIZATION; HETERODIMERS AB The biological effects of platelet-derived growth factor (PDGF) are mediated by cell surface alpha and beta PDGF receptors, which, as a result of ligand binding, undergo dimerization in a manner consistent with PDGF being bivalent. In order to directly demonstrate PDGF bivalency and to define the binding of PDGF AB to isolated beta receptor, we developed solid-phase binding assays using purified recombinant extracellular domain of human PDGF receptors. PDGF AA, AB, and BB were prepared from the monomeric chains expressed in Escherichia coli, and each was purified to homogeneity; PDGF AB contained <0.5% of either homodimer. The interactions of these isoforms with immobilized PDGF receptors were examined by several approaches. Scatchard analysis revealed high affinity binding (K(d) = 0.5-1.0 nM) of radiolabeled PDGF AA and AB to alpha receptor and of PDGF BB to both receptor subtypes. Contrary to previous reports, PDGF AB also bound beta receptor with high affinity (K(d) = 0.9 nM). When a B-chain-specific monoclonal antibody that recognizes the putative binding domain of PDGF BB was used for ligand detection, we found that PDGF AB binding to beta receptor occurred exclusively through the B-chain subunit, whereas binding to alpha receptor occurred through either subunit. In addition, site-directed mutagenesis was used to specifically inactivate the B chain of PDGF AB, which eliminated binding to the beta receptor without affecting alpha receptor binding. These results establish that PDGF is bivalent and that monovalent ligand retains high affinity receptor binding. C1 COR THERAPEUT INC,256 E GRAND AVE,S SAN FRANCISCO,CA 94080. NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. NR 46 TC 50 Z9 52 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3625 EP 3631 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100091 PM 7679113 ER PT J AU DEGUCHI, Y AGUS, D KEHRL, JH AF DEGUCHI, Y AGUS, D KEHRL, JH TI A HUMAN HOMEOBOX GENE, HB24, INHIBITS DEVELOPMENT OF CD4+ T-CELLS AND IMPAIRS THYMIC INVOLUTION IN TRANSGENIC MICE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSCRIPTION FACTOR; BONE-MARROW; B-CELLS; EXPRESSION; LYMPHOCYTES; HOX-1.1; REGION; BOX AB The HB24 gene encodes a diverged human homeodomain-containing protein known to be expressed in hematopoietic progenitors and activated lymphocytes. We have generated transgenic mice that express HB24 under the control of the T cell receptor beta chain promoter/enhancer. Analysis of T cells and thymocytes from the transgenic mice revealed a marked increase in activated cells as assessed by cell size profiles and interleukin-2 receptor expression. Within the thymus these changes were most pronounced in the CD4+CD8- subset. Strikingly, the normal development of CD4+ T cells in the transgenic mice was impaired. Single positive CD4 cells were reduced 35% in the thymus, and CD4+ T cells were reduced 90% in the spleen and lymph nodes compared to the controls. Similar findings were found both in young mice (6 weeks old) and in more elderly mice (1 y old). However, the thymuses of the elderly mice failed to undergo normal involution. Sera from HB24 transgenic mice had levels of IgG1 10-100-fold lower than sera from matched controls, most likely as a consequence of the decrease in CD4+ T cells. These transgenic mice provide a useful model for studying the role of HB24 in lymphocyte activation as well as for understanding the effects of abnormal T cell activation on thymic and T cell development. C1 NIAID,IMMUNOREGULAT LAB,BLDG 10,RM 11B-13,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,BETHESDA,MD 20892. OI Kehrl, John/0000-0002-6526-159X FU NICHD NIH HHS [N01-HD-02911] NR 38 TC 18 Z9 18 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3646 EP 3653 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100094 PM 8094082 ER PT J AU PARK, DG JHON, DY LEE, CW RYU, SH RHEE, SG AF PARK, DG JHON, DY LEE, CW RYU, SH RHEE, SG TI REMOVAL OF THE CARBOXYL-TERMINAL REGION OF PHOSPHOLIPASE-C-BETA-1 BY CALPAIN ABOLISHES ACTIVATION BY G-ALPHA-Q SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BOVINE BRAIN; G-PROTEINS; BETA-1 ISOZYME; RAT-BRAIN; PURIFICATION; FORMS AB The 150-kDa phospholipase C (PLC)-beta1 and three immunologically related proteins with molecular sizes of 140, 100, and 45 kDa were purified from bovine brain extracts. Determination of the amino-terminal amino acid sequence of the 45-kDa protein and immunoblots of the purified proteins with sequence-specific antibodies to peptides corresponding to three different regions of PLC-beta1 suggest that a single cleavage at the linkage between amino acid residues 880 and 881 of PLC-beta1 generates the 100- and 45-kDa proteins, which correspond to the amino-terminal and carboxyl-terminal portions, respectively, of PLC-beta1. The Ca2+-dependent protease calpain appears to be responsible for the cleavage of PLC-beta1; the PLC-beta1 amino acid sequence contains PEST sequences which are common to proteins susceptible to calpain, and limited proteolysis of purified PLC-beta1 by calpain generated a 100-kDa protein and a 40-kDa protein that contains the same amino-terminal sequence as the 45-kDa protein. The 140-kDa protein lacks the carboxyl-terminal-most region of PLC-beta1, but there is no evidence it is derived from PLC-beta1 by proteolysis. Cleavage of PLC-beta1 by calpain had no significant effect on catalytic activity measured in the absence of the alpha subunit of the Galphaq but completely abrogated the stimulatory effect of Galphaq. On the other hand, Galphaq activated the 140-kDa enzyme. These results suggest that the region between residue 881 and the most carboxyl-terminal 10 kDa of PLC-beta1 contains the Galphaq interaction site. C1 NHLBI,BIOCHEM LAB,BLDG 3,RM 122,BETHESDA,MD 20892. POHANG INST SCI & TECHNOL,DEPT LIFE SCI,POHANG 790600,SOUTH KOREA. NR 22 TC 126 Z9 126 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3710 EP 3714 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100103 PM 8429045 ER PT J AU WANG, F MARTIN, BM SELLERS, JR AF WANG, F MARTIN, BM SELLERS, JR TI REGULATION OF ACTOMYOSIN INTERACTIONS IN LIMULUS MUSCLE PROTEINS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SMOOTH-MUSCLE; THICK FILAMENTS; MYOSIN; ACTIN; CONTRACTION; VERTEBRATE; COMPLEX; KINASE; CHAIN AB Contraction of striated muscle from Limulus polyphemus, the horseshoe crab, is regulated by both calcium binding to a troponin-tropomyosin-dependent thin filament array and a myosin light chain kinase-dependent phosphorylation of myosin. We have isolated myosin from Limulus striated muscle and examined how these two regulatory systems affect the sliding velocity of actin filaments over myosin, using an in vitro motility assay. Our results show that in the presence of ATP, Limulus myosin must be phosphorylated in order to move actin filaments. No movement was observed for actin filaments interacting with dephosphorylated Limulus myosin. Calcium was not required for actin movement. In contrast, when both troponin and tropomyosin are bound to actin filaments, calcium is required for the movement of actin filaments over phosphorylated myosin. These results demonstrate that the ''off'' state of either the thin filament or thick filament regulatory system is dominant and that for the movement to occur, both phosphorylated Limulus myosin and an activated troponin-tropomyosin system are required. Tropomyosin by itself increases the sliding velocity of actin filaments over phosphorylated Limulus myosin about 10-fold in a calcium-independent manner. Tropomyosins from turkey gizzard smooth muscle, bovine cardiac muscle, and Limulus muscle all have a profound effect in increasing the velocity. Troponin alone does not change the velocity. Partial sequences of the tryptic phosphopeptides of Limulus myosin regulatory light chains generated following the phosphorylation by gizzard myosin light chain kinase yield ATS(PO4)NVFAMFEQNQIA for 21 kDa and SGS(PO4)NVFSMFT for 31-kDa light chain. C1 NIMH,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. RP WANG, F (reprint author), NHLBI,MOLEC CARDIOL LAB,BLDG 10,RM 8N202,BETHESDA,MD 20892, USA. NR 23 TC 22 Z9 22 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3776 EP 3780 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100113 PM 8429052 ER PT J AU FLOTTE, TR AFIONE, SA SOLOW, R DRUMM, ML MARKAKIS, D GUGGINO, WB ZEITLIN, PL CARTER, BJ AF FLOTTE, TR AFIONE, SA SOLOW, R DRUMM, ML MARKAKIS, D GUGGINO, WB ZEITLIN, PL CARTER, BJ TI EXPRESSION OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR FROM A NOVEL ADENOASSOCIATED VIRUS PROMOTER SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DEPENDENT PROTEIN-KINASE; TRANSCRIPTION FACTOR; CHLORIDE CHANNELS; ADENOASSOCIATED VIRUS; EPITHELIAL-CELLS; GENE; DNA; REPLICATION; INTEGRATION; CLONING AB Adeno-associated virus type 2 (AAV) vectors have been used for gene expression in respiratory epithelial cells and may be useful in gene therapy for diseases like cystic fibrosis (CF) which affect the airways. The AAV p5 promoter together with the AAV inverted terminal repeat (ITR) forms a 263-base pair cassette which mediated efficient expression in a CF bronchial epithelial cell line. We report here that the ITR itself can mediate gene expression. In stable transfection assays, AAV-CF vectors expressing the full-length cystic fibrosis transmembrane conductance regulator (CFTR) cDNA from either the p5 promoter or the ITR restored cAMP regulation of the chloride efflux characteristic of CFTR function. An AAV-ITR-CF vector deleted for the amino terminus of CFTR was also functional. This vector was packaged into AAV particles and used to transduce cells without selection. Transduced cells also exhibited cAMP-regulated Cl- efflux. The complemented cell lines showed increased levels of CFTR protein immunofluorescence, and the presence of intact AAV-CF vector sequence was confirmed by Southern blot analysis of rescued vector sequences. These studies provide.novel insights into AAV gene expression, and this newly described promoter allows for the production of AAV vectors expressing CFTR in those differentiated cells affected in CF. C1 TARGETED GENET CORP,SEATTLE,WA 98101. JOHNS HOPKINS MED INST,DEPT PHYSIOL,BALTIMORE,MD 21205. JOHNS HOPKINS MED INST,EUDOWOOD DIV PEDIAT RESP SCI,BALTIMORE,MD 21205. UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109. RP FLOTTE, TR (reprint author), NIDDKD,MOLEC & CELLULAR BIOL LAB,BETHESDA,MD 20892, USA. NR 48 TC 246 Z9 248 U1 1 U2 6 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 15 PY 1993 VL 268 IS 5 BP 3781 EP 3790 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KM161 UT WOS:A1993KM16100114 PM 7679117 ER PT J AU KRAKAUER, T OPPENHEIM, JJ AF KRAKAUER, T OPPENHEIM, JJ TI IL-1 AND TUMOR-NECROSIS-FACTOR-ALPHA EACH UP-REGULATE BOTH THE EXPRESSION OF IFN-GAMMA RECEPTORS AND ENHANCE IFN-GAMMA-INDUCED HLA-DR EXPRESSION ON HUMAN MONOCYTES AND A HUMAN MONOCYTIC CELL-LINE (THP-1) SO JOURNAL OF IMMUNOLOGY LA English DT Article ID PERIPHERAL-BLOOD MONOCYTES; INTERFERON-GAMMA; T-CELL; GROWTH-FACTOR; ANTIGEN EXPRESSION; IMMUNE INTERFERON; AUTOCRINE GROWTH; IA-ANTIGEN; B-CELLS; INDUCTION AB Stimulation of human blood monocytes (adherent mononuclear cells) and the monocytic cell line, THP-1, by IL-1 or TNF-alpha leads to the up-regulation of IFN-gamma receptors. Scatchard analysis using I-125-IFN-gamma revealed a twofold increase in the number of IFN-gamma receptors on THP-1 cells without an alteration in the affinity of the receptor. The potential functional significance of this induction of IFN-gamma receptors on monocytes and THP-1 cells was investigated by examining the effect of IFN-gamma on MHC class II Ag expression by these cells. Both IL-1 and TNF-alpha enhanced the IFN-gamma-induced HLA-DR expression (>twofold) and this effect was inhibited by antibody to IFN-gamma. In the case of human monocytes, IL-1 or TNF-alpha, each by themselves also increased HLA-DR expression, which was also abrogated by antibody to IFN-gamma. The data suggest that the immunopotentiating effects of IL-1 and TNF-alpha are mediated in part by enhancing IFN-gamma receptor expression on monocytes and macrophages. This presumably would increase the capacity of IFN-gamma to activate macrophages, enabling them to express HLA-DR and present Ag more effectively. C1 NCI,FREDERICK CANC RES & DEV CTR,MOLEC IMMUNOREGULAT LAB,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21702. RP KRAKAUER, T (reprint author), USAMRIID,DIV MED,BLDG 1425,FT DETRICK,FREDERICK,MD 21702, USA. NR 45 TC 102 Z9 103 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1993 VL 150 IS 4 BP 1205 EP 1211 PG 7 WC Immunology SC Immunology GA KM706 UT WOS:A1993KM70600006 PM 8432976 ER PT J AU SHORES, EW NAKAYAMA, T WIEST, DL TAKAHAMA, Y SHARROW, S SINGER, A AF SHORES, EW NAKAYAMA, T WIEST, DL TAKAHAMA, Y SHARROW, S SINGER, A TI STRUCTURALLY DISTINCT T-CELL RECEPTOR COMPLEXES ON DEVELOPMENTALLY DISTINCT T-CELL POPULATIONS IN SEVERE COMBINED IMMUNODEFICIENCY MICE EXPRESSING A TCR-BETA TRANSGENE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID COMBINED IMMUNE-DEFICIENCY; ANTIGEN RECEPTOR; MONOCLONAL-ANTIBODY; SCID MICE; TYROSINE PHOSPHORYLATION; SURFACE EXPRESSION; DELTA-CHAIN; THYMOCYTES; ALPHA; PROTEINS AB T cell differentiation and TCR expression were assessed in severe combined immunodeficiency (SCID) mice possessing an already rearranged TCRVbeta8 transgene. Unlike nontransgenic SCID mice, TCRVbeta8-transgenic SCID mice contained a broad spectrum of T cell populations, including both immature thymocytes and mature T cells. In TCRVbeta8-transgenic SCID mice, immature CD4-CD8- and CD4+CD8+ thymocytes expressed surface TCR complexes structurally distinct from those expressed by mature single-positive T. cells. Immature CD4+CD8+ thymocytes expressed surface TCRbeta chains without a clonotypic TCR partner chain and largely without associated CD3 components. In contrast, mature single-positive T cells expressed fully assembled surface TCR complexes containing disulfide-linked heterodimers consisting of transgenic TCRbeta chains and endogenous TCRalpha chains. The surface TCR complexes on mature T cells were associated with CD3 components and were competent to transduce TCR-mediated proliferative signals. Thus, T cells at different stages of development in TCRVbeta8-transgenic SCID mice express structurally distinct surface TCR complexes, demonstrating that the developmental stage achieved by T cells in these mice is related to the structure of the surface TCR complexes they express. Indeed, the present results indicate that successful differentiation into single-positive T cells requires surface expression of fully assembled TCR complexes. RP SHORES, EW (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4817,BETHESDA,MD 20892, USA. RI Nakayama, Toshinori/E-1067-2017; OI Wiest, David/0000-0002-0792-3188 NR 58 TC 26 Z9 26 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1993 VL 150 IS 4 BP 1263 EP 1275 PG 13 WC Immunology SC Immunology GA KM706 UT WOS:A1993KM70600012 PM 8094405 ER PT J AU IKEMATSU, H HARINDRANATH, N UEKI, Y NOTKINS, AL CASALI, P AF IKEMATSU, H HARINDRANATH, N UEKI, Y NOTKINS, AL CASALI, P TI CLONAL ANALYSIS OF A HUMAN-ANTIBODY RESPONSE .2. SEQUENCES OF THE VH GENES OF HUMAN-IGM, IGG, AND IGA TO RABIES VIRUS REVEAL PREFERENTIAL UTILIZATION OF VHIII SEGMENTS AND SOMATIC HYPERMUTATION SO JOURNAL OF IMMUNOLOGY LA English DT Article ID IMMUNOGLOBULIN HEAVY-CHAIN; VARIABLE REGION GENES; SYSTEMIC LUPUS-ERYTHEMATOSUS; EPSTEIN-BARR VIRUS; B-CELL REPERTOIRE; GERM-LINE GENES; IMMUNE-RESPONSE; RHEUMATOID FACTORS; NUCLEOTIDE-SEQUENCES; PRIMARY IMMUNIZATION AB The construction of mAb-producing cell lines has been instrumental in dissecting the fine specificities and genetic makeup of murine antibodies to exogenous and self Ag. The analysis of the genetic composition of human antibody responses has been hampered by the difficulty in generating human mAb of predetermined class and specificity. Using B lymphocytes from three healthy subjects vaccinated with inactivated rabies virus vaccine, we generated nine human mAb binding to rabies virus and analyzed the genes encoding their V(H) regions. Six mAb (five IgG1 and one IgA1) were monoreactive and displayed high affinities for rabies virus Ag. The remaining three mAb (IgM) were polyreactive and displayed lower affinities for rabies virus Ag. Seven mAb (3 IgG1, the IgA1, and the three IgM) utilized V(H) gene segments of the V(H)III family. The remaining two IgG1 mAb utilized gene segments of the V(H)I and V(H)IV families. Of the seven expressed V(H)III family genes, three were similar to the germline V(H)26c gene, two to the germline 22-2B gene, one to the germline H11 gene, and one to the germline 8-1B gene. The expressed V(H)I and V(H)IV genes displayed sequences similar to those of the germline hv1263 and V71-4 genes, respectively. The V(H) genes of all but one mAb (mAb55) resembled those that are predominantly expressed by Cmu+ clones in human fetal liver libraries. When compared with known germline sequences, the V(H) genes of the rabies virus-binding mAb displayed variable numbers of nucleotide differences. That such differences resulted from a process of somatic hypermutation was formally demonstrated (by analyzing DNA from polymorphonuclear neutrophil of the same subject whose B lymphocytes were used for the mAb generation) in the case of the V(H) gene of the high affinity (anti-rabies virus glycoprotein) IgG1 mAb57 that has been shown to efficiently neutralize the virus in vitro and in vivo. The distribution, mainly within the complementarity determining regions, and the high replacement-to-silent ratio of the mutations, were consistent with the hypothesis that the mAb57-producing cell clone underwent a process of Ag-driven affinity maturation through clonal selection. The D gene segments of the rabies virus-selected mAb were heterogeneous and, in most cases, flanked by significant N segment additions. The J(H) segment utilization was unbalanced and reminiscent of those of the adult and fetus. Four mAb utilized J(H)4, two J(H)6, two J(H)3, and one J(H)5; no mAb utilized J(H)1 or J(H)2 genes. The present data suggest that the adult human Ig V gene assortment expressed as the result of selection by a proteinic mosaic Ag is more restricted than previously assumed and resembles that of the putatively unselected adult B cell repertoire and the unselected Cmu+ cell repertoire of the fetus. They also document somatic Ig V gene hypermutation in human B cells producing high affinity antibodies. C1 NYU, SCH MED, DEPT PATHOL, MSB 599, 550 1ST AVE, NEW YORK, NY 10016 USA. NYU, SCH MED, KAPLAN COMPREHENS CANC CTR, NEW YORK, NY 10016 USA. NIDR, ORAL MED LAB, BETHESDA, MD 20892 USA. RI Casali, Paolo/F-6579-2010 FU NCI NIH HHS [CA-16087, P30 CA016087]; NIAMS NIH HHS [AR-40908, R01 AR040908] NR 81 TC 93 Z9 93 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1993 VL 150 IS 4 BP 1325 EP 1337 PG 13 WC Immunology SC Immunology GA KM706 UT WOS:A1993KM70600018 PM 8432980 ER PT J AU NISSIM, A SCHWARZBAUM, S SIRAGANIAN, R ESHHAR, Z AF NISSIM, A SCHWARZBAUM, S SIRAGANIAN, R ESHHAR, Z TI FINE SPECIFICITY OF THE IGE INTERACTION WITH THE LOW AND HIGH-AFFINITY FC RECEPTOR SO JOURNAL OF IMMUNOLOGY LA English DT Article ID HUMAN IMMUNOGLOBULIN-E; CELL BINDING-SITE; RAT MAST-CELLS; MONOCLONAL-ANTIBODIES; EPSILON RECEPTOR; MURINE IGE; EPITOPES; DOMAIN; LOCALIZATION; EXPRESSION AB The characterization of the site(s) on the IgE molecule that accommodate the high (FcepsilonRI) and low (FcepsilonRII) affinity receptors for IgE should allow the design of IgE analogues that can be used to block the onset of the allergic response or to regulate IgE production. To identify the IgE domain responsible for receptor binding, we generated a series of chimeric IgE antibodies in which constant region domains were interchanged between the human and mouse molecules. Binding studies with these chimeras revealed that both the high and low affinity receptor binding-sites reside primarily in the third constant domain of IgE (Cepsilon3). Additional chimeric IgE molecules were generated in which different parts of the human Cepsilon3 domain were exchanged with their murine homologues. Binding experiments with these chimeras suggest that not only the sequence of a particular Cepsilon3 fragment, but the entire Cepsilon3 domain in its native configuration is essential for binding to the FcepsilonRI. The amino acid residues determining the species specificity of the FcepsilonERII are not contained in the first 16 amino acids of the Cepsilon3 domain. C1 WEIZMANN INST SCI,DEPT CHEM IMMUNOL,IL-76100 REHOVOT,ISRAEL. NIDR,IMMUNOL LAB,CLIN IMMUNOL SECT,BETHESDA,MD 20892. NR 27 TC 36 Z9 37 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1993 VL 150 IS 4 BP 1365 EP 1374 PG 10 WC Immunology SC Immunology GA KM706 UT WOS:A1993KM70600022 PM 7679426 ER PT J AU HODES, RJ NOVICK, MB PALMER, LD KNEPPER, JE AF HODES, RJ NOVICK, MB PALMER, LD KNEPPER, JE TI ASSOCIATION OF A V-BETA-2-SPECIFIC SUPERANTIGEN WITH A TUMORIGENIC MILK-BORNE MOUSE MAMMARY-TUMOR VIRUS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID REACTIVE T-CELLS; CLONAL DELETION; MLS; TOLERANCE; MICE; EXPRESSION; ANTIGENS; GENES AB A number of endogenous mouse mammary tumor virus (MMTV) proviruses encode superantigens that have the property of stimulating mature T lymphocytes in a TCR Vbeta-specific fashion and of mediating Vbeta-specific clonal deletion in developing T cells. The tumorigenic milk-borne MMTV carried by C3H and GR mice also have superantigen properties in vivo, and it has been proposed that this superantigenic function is critical to the infectivity and/or tumorigenicity of the virus. To test the requirement for superantigen properties in tumorigenic MMTV, a highly tumorigenic strain of MMTV isolated from BALB/c mice (BALB/cV virus) was analyzed for its effect on TCR Vbeta expression. It was found that exposure of newborn mice to milk-borne virus results in marked deletion of Vbeta2-expressing CD4+ peripheral T cells. This deletion is detected in mature TCR(high) thymocytes as well as in peripheral T cells from virus-exposed mice. Deletion is dependent on expression of a permissive MHC type in mice exposed to virus. Subcutaneous injection of adult mice with virus-containing milk induces a substantial increase in Vbeta2+ CD4+ cells in draining lymph nodes within 4 days. Thus, tumorigenic BALB/cV is associated with Vbeta2-specific superantigen activity capable of mediating both T cell expansion and clonal deletion in vivo. These findings are consistent with a critical role of superantigen-mediated T cell activation in MMTV infection and tumorigenesis. C1 VILLANOVA UNIV,DEPT BIOL,VILLANOVA,PA 19085. RP HODES, RJ (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,RM 4B17,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA-52853] NR 24 TC 31 Z9 31 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 15 PY 1993 VL 150 IS 4 BP 1422 EP 1428 PG 7 WC Immunology SC Immunology GA KM706 UT WOS:A1993KM70600028 PM 8094408 ER PT J AU LLOYD, AR AF LLOYD, AR TI THE ECONOMIC-IMPACT OF CHRONIC FATIGUE SYNDROME - REPLY SO MEDICAL JOURNAL OF AUSTRALIA LA English DT Letter RP LLOYD, AR (reprint author), NCI,MOLEC IMMUNOREGULAT LAB,BLDG 560,ROOM 31-19,FREDERICK,MD 21702, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AUSTRALASIAN MED PUBL CO LTD PI SYDNEY PA LEVEL 1, 76 BERRY ST, SYDNEY NSW 2060, AUSTRALIA SN 0025-729X J9 MED J AUSTRALIA JI Med. J. Aust. PD FEB 15 PY 1993 VL 158 IS 4 BP 286 EP 287 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA KM460 UT WOS:A1993KM46000027 ER PT J AU WISTOW, GJ SHAUGHNESSY, MP LEE, DC HODIN, J ZELENKA, PS AF WISTOW, GJ SHAUGHNESSY, MP LEE, DC HODIN, J ZELENKA, PS TI A MACROPHAGE-MIGRATION INHIBITORY FACTOR IS EXPRESSED IN THE DIFFERENTIATING CELLS OF THE EYE LENS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID FIBROBLAST GROWTH-FACTOR; ENDOTHELIAL-CELLS; TRANSGENIC MICE; CRYSTALLINS; EXTENSION; PROTEINS; CLONING; GENE AB A discrete 10-kDa polypeptide (10K) is expressed from early stages in the embryonic chicken lens. Since this has potential as a marker for lens cell development, chicken 10K and its homologues from mouse and human lenses were identified by protein sequencing and cloning. Surprisingly, lens 10K proteins appear to be identical to a lymphokine, macrophage migration inhibitory factor (MIF), originally identified in activated human T cells. Using microdissection and PCR techniques, we find that expression of 10K/MIF is strongly correlated with cell differentiation in the developing chicken lens. Northern blot analysis shows that 10K/MIF is widely expressed in mouse tissues. These results suggest that proteins with MIF activity may have roles beyond the immune system, perhaps as intercellular messengers or part of the machinery of differentiation itself. Indeed, partial sequence of other small lens proteins identifies another MIF-related protein (MRP8) in calf lens. The relatively abundant expression of MIF in lens may have clinical significance, with the possibility of involvement in ocular inflammations that may follow damage to the lens. C1 NEI,CELLULAR DIFFERENTAT SECT,MOLEC & DEV BIOL LAB,BETHESDA,MD 20892. RP WISTOW, GJ (reprint author), NEI,MOLEC STRUCT & FUNCT SECT,MOLEC & DEV BIOL LAB,ROOM 222,BLDG 6,BETHESDA,MD 20892, USA. NR 28 TC 167 Z9 172 U1 1 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1272 EP 1275 DI 10.1073/pnas.90.4.1272 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700026 PM 7679497 ER PT J AU TORRENCE, PF MAITRA, RK LESIAK, K KHAMNEI, S ZHOU, A SILVERMAN, RH AF TORRENCE, PF MAITRA, RK LESIAK, K KHAMNEI, S ZHOU, A SILVERMAN, RH TI TARGETING RNA FOR DEGRADATION WITH A (2'-5')OLIGOADENYLATE-ANTISENSE CHIMERA SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID MESSENGER-RNA; PROTEIN-SYNTHESIS; DNA; INHIBITION; CELLS; OLIGONUCLEOTIDES; EXPRESSION; 2-5A; OLIGODEOXYRIBONUCLEOTIDES; REPLICATION AB Antisense oligonucleotides hold considerable promise both as research tools for inhibiting gene expression and as agents for the treatment of a myriad of human diseases. However, targeted destruction of RNA has been difficult to achieve in a versatile, efficient, and reliable manner. We have developed an effective strategy for cleaving unique RNA sequences with 2-5A-dependent RNase, an endoribonuclease that mediates inhibitory effects of interferon on virus infection and is activated by 5'-phosphorylated 2'-5'-linked oligoadenylates known as 2-5A [p(n)5'A2'(p5'A2')(m)p5'A], resulting in the cleavage of single-stranded RNA predominantly after UpUp and UpAp sequences. To direct 2-5A-dependent RNase to cleave unique RNA sequences, p5'A2'p5'A2'p5'A2'p5'A was covalently linked to an antisense oligonucleotide to yield a chimeric molecule (2-5A:AS). The antisense oligonucleotide component of 2-5A:AS bound a specific RNA sequence while the accompanying 2-5A component activated 2-5A-dependent RNase, thereby causing the cleavage of the RNA in the targeted sequence. This strategy was demonstrated by inducing specific cleavage within a modified human immunodeficiency virus type 1 vif mRNA in a cell-free system from human lymphoblastoid cells. Because 2-5A-dependent RNase is present in most mammalian cells, the control of gene expression based on this technology-including therapies for cancer, viral infections, and certain genetic diseases-can be envisioned. C1 CLEVELAND CLIN FDN,RES INST,DEPT CANC BIOL,CLEVELAND,OH 44195. RP TORRENCE, PF (reprint author), NIDDKD,MED CHEM LAB,BIOMED CHEM SECT,BLDG 8,ROOM B2A02,BETHESDA,MD 20892, USA. FU NIAID NIH HHS [5 R01 AI28253] NR 38 TC 104 Z9 105 U1 1 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1300 EP 1304 DI 10.1073/pnas.90.4.1300 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700032 PM 7679499 ER PT J AU SWAIN, AL JASKOLSKI, M HOUSSET, D RAO, JKM WLODAWER, A AF SWAIN, AL JASKOLSKI, M HOUSSET, D RAO, JKM WLODAWER, A TI CRYSTAL-STRUCTURE OF ESCHERICHIA-COLI L-ASPARAGINASE, AN ENZYME USED IN CANCER-THERAPY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE AMIDOHYDROLASE; LEUKEMIA; ACTIVE SITE; ASPARTATE ID GLUTAMINASE-ASPARAGINASE; MAGNETIC-RESONANCE; BINDING; ACINETOBACTER; RESOLUTION; SITE AB The crystal structure of Escherichia coli asparaginase II (EC 3.5.1.1), a drug (Elspar) used for the treatment of acute lymphoblastic leukemia, has been determined at 2.3 angstrom resolution by using data from a single heavy atom derivative in combination with molecular replacement. The atomic model was refined to an R factor of 0.143. This enzyme, active as a homotetramer with 222 symmetry, belongs to the class of alpha/beta proteins. Each subunit has two domains with unique topological features. On the basis of present structural evidence consistent with previous biochemical studies, we propose locations for the active sites between the N- and C-terminal domains belonging to different subunits and postulate a catalytic role for Thr-89. C1 NCI,FREDERICK CANC RES & DEV CTR,MACROMOLEC STRUCT LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101, F32 CA 08909-02] NR 31 TC 156 Z9 169 U1 2 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1474 EP 1478 DI 10.1073/pnas.90.4.1474 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700068 PM 8434007 ER PT J AU DIBRINO, M PARKER, KC SHILOACH, J KNIERMAN, M LUKSZO, J TURNER, RV BIDDISON, WE COLIGAN, JE AF DIBRINO, M PARKER, KC SHILOACH, J KNIERMAN, M LUKSZO, J TURNER, RV BIDDISON, WE COLIGAN, JE TI ENDOGENOUS PEPTIDES BOUND TO HLA-A3 POSSESS A SPECIFIC COMBINATION OF ANCHOR RESIDUES THAT PERMIT IDENTIFICATION OF POTENTIAL ANTIGENIC PEPTIDES SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I; BETA-2-MICROGLOBULIN; PEPTIDE BINDING MOTIF ID HLA-B27; PROTEIN; CELLS AB A motif specific to peptides that bind to the human class I major histocompatibility complex molecule HLA-A3 was identified by sequence analysis of HPLC fractions containing endogenous peptides. Twenty-six different sequences were obtained, 19 of which were nonamers. The majority of these endogenous peptide sequences contained Leu at position (P)2, while most sequences contained Tyr or Lys at P9. In addition, Phe was shared by 16 sequences at P3. Synthetic peptides corresponding to endogenous peptide sequences were shown to bind to HLA-A3. The importance of Leu at P2 and Tyr or Lys at P9 (''anchor'' residues) for peptide binding to HLA-A3 was demonstrated by the following results: (i) peptides GLFGGGGGY, GLFGGGGGK, and GLGGGGFGY, but not GLFGGGGGV, specifically bound to HLA-A3 and (ii) six nonapeptides from within the influenza A nucleoprotein, matrix, and polymerase proteins, selected for synthesis based upon their possession of P2 and P9 anchor residues, were shown to bind HLA-A3. In contrast, none of a set of eight peptides that bound to HLA-A2, or six that bound to HLA-B27, bound detectably to HLA-A3. These findings provide a rationale for the design and selection of peptides that can be recognized by HLA-A3-restricted T cells. C1 NIAID,BIOL RESOURCES BRANCH,BLDG 4,ROOM 413,BETHESDA,MD 20892. NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892. NINCDS,MOLEC IMMUNOL SECT,NEUROIMMUNOL BRANCH,BETHESDA,MD 20892. OI Parker, Kenneth/0000-0002-6282-2478 NR 16 TC 115 Z9 117 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1508 EP 1512 DI 10.1073/pnas.90.4.1508 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700075 PM 7679507 ER PT J AU STEELE, FR CHADER, GJ JOHNSON, LV TOMBRANTINK, J AF STEELE, FR CHADER, GJ JOHNSON, LV TOMBRANTINK, J TI PIGMENT EPITHELIUM-DERIVED FACTOR - NEUROTROPHIC ACTIVITY AND IDENTIFICATION AS A MEMBER OF THE SERINE PROTEASE INHIBITOR GENE FAMILY SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID RETINOBLASTOMA; CELLS AB Cultured pigment epithelial cells of the fetal human retina secrete a protein, pigment epithelium-derived factor (PEDF), that induces a neuronal phenotype in cultured human retinoblastoma cells. Morphological changes include the induction of an extensive neurite meshwork and the establishment of corona-like cellular aggregates surrounding a central lumen. The differentiated cells also show increases in the expression of neuron-specific enolase and the 200-kDa neurofilament subunit. Amino acid and DNA sequence data demonstrate that PEDF belongs to the serine protease inhibitor (serpin) family. The PEDF gene contains a typical signal-peptide sequence, initiator methionine codon, and polyadenylylation signal and matches the size of other members of the serpin superfamily (e.g., alpha1-antitrypsin). It lacks homology, however, at the putative serpin reactive center. Thus, PEDF could exert a paracrine effect in the embryonic retina, influencing neuronal differentiation by a mechanism that does not involve classic inhibition of serine protease activity. C1 NEI,RETINAL CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. UNIV SO CALIF,SCH MED,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90033. NR 23 TC 321 Z9 343 U1 0 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1526 EP 1530 DI 10.1073/pnas.90.4.1526 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700079 PM 8434014 ER PT J AU NASONBURCHENAL, K WOLFF, L AF NASONBURCHENAL, K WOLFF, L TI ACTIVATION OF C-MYB IS AN EARLY BONE-MARROW EVENT IN A MURINE MODEL FOR ACUTE PROMONOCYTIC LEUKEMIA SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE PROTOONCOGENE; PRELEUKEMIA; INFLAMMATION; REVERSE TRANSCRIPTION-PCR ID MESSENGER-RNA; BALB/C MICE; TUMORS; VIRUS; CLASSIFICATION; SEQUENCE; CELLS AB Insertional mutagenesis of c-myb by Moloney murine leukemia virus occurs in 100% of promonocytic leukemias (MMLS) induced by the virus. These leukemias, which resemble acute monocytic leukemia-M5 in humans are induced only in mice undergoing a peritoneal chronic inflammatory response. We have found that two leukemia-specific gag-myb mRNAs in MML provide molecular markers for detection of preleukemic cells in hematopoietic tissue in vivo. The two aberrant RNAs result from splicing of gag to either exon 3 or 4 of c-myb, depending on the site of proviral integration. After reverse transcription-PCR with nested primers and hybridization with specific gag-myb junction probes, one cell, having aberrant c-myb message, could be detected in a minimum of 10(5) liver cells or 10(6) spleen or bone-marrow cells. This approach was used to examine hematopoietic tissues of mice after pristane injection to induce inflammation and virus inoculation. Cells with gag-myb mRNAs could be detected as early as 2 weeks after virus inoculation. In mice receiving both pristane and virus, there was evidence of preleukemic cells in 83% of the mice by 3 weeks after virus infection. Furthermore, 100% of the mice were positive for preleukemic cells by 8 weeks, even though only 50% of mice have been shown to succumb to MML (peak time for disease latency is 12-16 weeks). Cells with these aberrant c-myb messages were initially detected in the bone marrow, but during intermediate stages of disease development these cells disseminated to the spleen, liver, and granuloma. At preleukemic times, from 3 to 8 weeks after virus infection, a lower percentage of mice were positive in the group that did not receive pristane compared with mice in the group receiving pristane. However, at 18 weeks, 100% of the mice in the group receiving virus only had evidence of cells expressing gag-myb RNA in their spleens and/or bone marrow; it is of interest that mice inoculated with virus alone never develop MML. This approach for detecting preleukemic cells will now allow the study of mechanisms by which these preleukemic cells progress to a more transformed state and, perhaps, to a more differentiated state. C1 NCI,GENET LAB,BETHESDA,MD 20892. CATHOLIC UNIV AMER,DEPT BIOL,WASHINGTON,DC 20064. NR 21 TC 45 Z9 45 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 15 PY 1993 VL 90 IS 4 BP 1619 EP 1623 DI 10.1073/pnas.90.4.1619 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KM607 UT WOS:A1993KM60700098 PM 7679511 ER PT J AU LE, SY CHEN, JH MAIZEL, JV AF LE, SY CHEN, JH MAIZEL, JV TI IDENTIFICATION OF UNUSUAL RNA FOLDING PATTERNS ENCODED BY BACTERIOPHAGE-T4 GENE 60 SO GENE LA English DT Article DE RNA STRUCTURE; MESSENGER RNA TRANSLATION; TOPOISOMERASE; CODING GAP BYPASS ID RIBOSOMAL FRAMESHIFTING SIGNAL; PHENYLALANINE TRANSFER-RNA; MESSENGER-RNA; CRYSTALLOGRAPHIC REFINEMENT; STATISTICAL SIGNIFICANCE; TRANSLATIONAL CONTROL; SECONDARY STRUCTURE; VIRUS; SEQUENCE; YEAST AB A 50-nucleotide (nt) untranslated region (coding gap sequence) that interrupts the amino acid coding sequence in T4 gene 60, plus an additional 5 nt upstream and another 3 nt downstream from the gap sequence, shows unusual folding patterns according to RNA structure prediction. A predicted highly stable and significant hairpin structure in the 5' half of the gap sequence and a plausible tertiary structural element computed in the 3' part of the gap sequence seem significant by statistical tests on the wild-type (wt) sequence. This feature is absent in insertion, deletion and substitution variants of the gap sequence, in which template activities are markedly lower than that of the wt. The proposed feature is consistent with currently available data showing that the translational bypass of the coding gap is correlated with a stop codon involved in a stem-loop structure folded in the gap sequence. We suggest that the role of this segment in 'ribosomal bypass' of a portion of the mRNA sequence is a property of its special folded structure. C1 NCI,CTR DIAG,FREDERICK,MD 21702. NCI,FCRDC,DYNCORP,PROGRAM RESOURCES INC,CTR BIOMED SUPERCOMP,FREDERICK,MD 21702. RP LE, SY (reprint author), NCI,DIV CANC BIOL,MATH BIOL LAB,BLDG 469,RM 151,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74102] NR 38 TC 10 Z9 13 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD FEB 14 PY 1993 VL 124 IS 1 BP 21 EP 28 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA KN909 UT WOS:A1993KN90900003 PM 8382655 ER PT J AU MIDDAUGH, CR STEADMAN, BL SCHURTENBERGER, P LEE, DC CHLEBOWSKI, JF AF MIDDAUGH, CR STEADMAN, BL SCHURTENBERGER, P LEE, DC CHLEBOWSKI, JF TI CHANGES IN STRUCTURE OF ALPHA-2-MACROGLOBULIN UPON REACTION WITH TRYPSIN AS ASSESSED BY LIGHT-SCATTERING AND DIFFERENTIAL SCANNING CALORIMETRY SO BIOCHIMICA ET BIOPHYSICA ACTA LA English DT Article DE ALPHA-2-MACROGLOBULIN; TRYPSIN; LIGHT-SCATTERING; CALORIMETRY; DSC; CONFORMATIONAL CHANGE ID X-RAY-SCATTERING; CONFORMATIONAL-CHANGES; FAST FORMS; METHYLAMINE; COMPLEXES; PLASMIN; BINDING; PURIFICATION; PROTEINASES; MICROSCOPY AB Employing a combination of static and dynamic light scattering, as well as differential scanning calorimetry (DSC), the structural changes which appear in alpha2-macroglobulin (alpha2M) upon trypsin binding have been further characterized. Light-scattering measurements suggest that a 15% reduction in both the hydrodynamic radius and radius of gyration occurs when two molecules of trypsin complex to alpha2M. Approx. 85% of this trypsin-induced compaction results from the binding of the first proteinase. A complementary result was obtained from DSC measurements in which the major fraction of the trypsin-induced conversion of alpha2M to a single more thermally stable form results from interaction with the first proteinase molecule. These observations support a functionally asymmetric model of trypsin binding to alpha2M in which the significant reduction in size of the complex is primarily due to the initial interaction of alpha2M with a single proteinase molecule. C1 SWISS FED INST TECHNOL,INST POLYMERE,CH-8092 ZURICH,SWITZERLAND. NEI,DEV & MOLEC BIOL LAB,BETHESDA,MD 20892. VIRGINIA COMMONWEALTH UNIV,DEPT BIOCHEM & MOLEC BIOPHYS,RICHMOND,VA 23284. RP MIDDAUGH, CR (reprint author), MERCK RES LABS,WP26-331,W POINT,PA 19486, USA. RI Schurtenberger, Peter/K-1777-2013 OI Schurtenberger, Peter/0000-0002-2790-8831 FU PHS HHS [27493] NR 33 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-3002 J9 BIOCHIM BIOPHYS ACTA PD FEB 13 PY 1993 VL 1161 IS 2-3 BP 328 EP 332 DI 10.1016/0167-4838(93)90233-H PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA KN129 UT WOS:A1993KN12900030 PM 7679290 ER PT J AU DELLAPUPPA, A KIMES, AS LONDON, ED AF DELLAPUPPA, A KIMES, AS LONDON, ED TI DOSE-DEPENDENT EFFECTS OF D-N-ALLYLNORMETAZOCINE ON REGIONAL CEREBRAL METABOLIC RATES FOR GLUCOSE SO BRAIN RESEARCH LA English DT Article DE SIGMA-RECEPTOR; PSYCHOMOTOR STIMULANT; PHENCYCLIDINE; DEOXYGLUCOSE; CEREBRAL GLUCOSE METABOLISM ID DISCRIMINATIVE STIMULUS PROPERTIES; GUINEA-PIG BRAIN; PRECIPITATED MORPHINE-WITHDRAWAL; PHENCYCLIDINE RECEPTOR-BINDING; SIGMA-OPIATE RECEPTORS; ANTIPSYCHOTIC-DRUGS; CONSCIOUS RAT; REINFORCING PROPERTIES; MOUSE-BRAIN; SITES AB Cerebral metabolic patterns produced by different doses of the benzomorphan opioid drug, D-N-allylnormetazocine (D-NANM), were studied using the 2-deoxy-D-[1-C-14]glucose method in rats. The lowest dose of D-NANM (0.5 mg/kg) decreased regional cerebral metabolic rates for glucose (rCMRglc) in areas, such as cranial nerve nuclei, that contain high densities of sigma (sigma) receptors. However, higher doses of the drug (2.7 and 5 mg/kg) increased rCMRglc in components of the extrapyramidal motor and limbic systems. Some of these latter areas (e.g. molecular layer of the dentate gyrus, accumbens nucleus, globus pallidus, ventral posterior nucleus of the thalamus) are not enriched in sigma receptors. Reductions in rCMRglc produced by the lowest dose Of D-NANM probably reflect direct interactions of the drug with sigma receptors, whereas increases in rCMRglc observed with the highest doses more likely result from effects Of D-NANM on PCP receptors. C1 NIDA,ADDICT RES CTR,NEUROIMAGING & DRUG ACT SECT,POB 5180,BALTIMORE,MD 21224. NR 49 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD FEB 12 PY 1993 VL 603 IS 1 BP 38 EP 46 DI 10.1016/0006-8993(93)91297-6 PG 9 WC Neurosciences SC Neurosciences & Neurology GA KL916 UT WOS:A1993KL91600005 PM 8453477 ER PT J AU ALLEN, RC ARMITAGE, RJ CONLEY, ME ROSENBLATT, H JENKINS, NA COPELAND, NG BEDELL, MA EDELHOFF, S DISTECHE, CM SIMONEAUX, DK FANSLOW, WC BELMONT, J SPRIGGS, MK AF ALLEN, RC ARMITAGE, RJ CONLEY, ME ROSENBLATT, H JENKINS, NA COPELAND, NG BEDELL, MA EDELHOFF, S DISTECHE, CM SIMONEAUX, DK FANSLOW, WC BELMONT, J SPRIGGS, MK TI CD40 LIGAND GENE DEFECTS RESPONSIBLE FOR X-LINKED HYPER-IGM SYNDROME SO SCIENCE LA English DT Article ID HUMAN B-CELLS; HYPERIMMUNOGLOBULINEMIA-M; T-CELLS; IMMUNODEFICIENCY; INTERLEUKIN-4; DIFFERENTIATION; SWITCH; PROLIFERATION; LOCALIZATION; ACTIVATION AB The ligand for CD40 (CD40L) is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. Abnormalities in the CD40L gene were associated with an X-linked immunodeficiency in humans [hyper-IgM (immunoglobulin M) syndrome]. This disease is characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. CD40L complementary DNAs from three of four patients with this syndrome contained distinct point mutations. Recombinant expression of two of the mutant CD40L complementary DNAs resulted in proteins incapable of binding to CD40 and unable to induce proliferation or IgE secretion from normal B cells. Activated T cells from the four affected patients failed to express wild-type CD40L, although their B cells responded normally to wild-type CD40L. Thus, these CD40L defects lead to a T cell abnormality that results in the failure of patient B cells to undergo immunoglobulin class switching. C1 IMMUNEX RES & DEV CORP, DEPT MOLEC BIOL, SEATTLE, WA 98101 USA. BAYLOR COLL MED, HOWARD HUGHES MED INST, INST MOLEC GENET, HOUSTON, TX 77030 USA. IMMUNEX RES & DEV CORP, DEPT IMMUNOL, SEATTLE, WA 98101 USA. ST JUDE CHILDRENS RES HOSP, DEPT IMMUNOL, MEMPHIS, TN 38105 USA. NCI, FREDERICK CANC RES & DEV CTR, MAMMALIAN GENET LAB, ABL BASIC RES PROGRAM, FREDERICK, MD 21702 USA. UNIV WASHINGTON, DEPT PATHOL, SEATTLE, WA 98195 USA. UNIV TENNESSEE, CTR HLTH SCI, COLL MED, DEPT PEDIAT, MEMPHIS, TN 38163 USA. BAYLOR COLL MED, DEPT PEDIAT, HOUSTON, TX 77030 USA. OI Belmont, John/0000-0001-7409-3578 FU NCI NIH HHS [N01-CO-74101]; PHS HHS [A125129] NR 40 TC 676 Z9 685 U1 1 U2 10 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD FEB 12 PY 1993 VL 259 IS 5097 BP 990 EP 993 DI 10.1126/science.7679801 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KL800 UT WOS:A1993KL80000049 PM 7679801 ER PT J AU ZHOU, XL SASAKI, H LOWE, L HOGAN, BLM KUEHN, MR AF ZHOU, XL SASAKI, H LOWE, L HOGAN, BLM KUEHN, MR TI NODAL IS A NOVEL TGF-BETA-LIKE GENE EXPRESSED IN THE MOUSE NODE DURING GASTRULATION SO NATURE LA English DT Article ID CPG ISLANDS; FATE AB DURING gastrulation, the three germ layers of the embryo are formed and organized along the anterior-posterior body axis. In the mouse, gastrulation involves the delamination of ectodermal cells through the primitive streak and their differentiation into mesoderm1. These processes do not occur in embryos homozygous for a retrovirally induced recessive prenatal lethal mutation, the strain 413-d insertional mutation2,3. Instead of giving rise to mesoderm, embryonic ectoderm in 413-d mutants overproliferates and then rapidly degenerates, although extraembryonic lineages remain viable2. Here we isolate a candidate for the mutated gene which encodes a new member of the transforming growth factor-beta (TGF-beta) superfamily4 . Expression is first detected in primitive streak-stage embryos at about the time of mesoderm formation. It then becomes highly localized in the node at the anterior of the primitive streak. This region is analogous to chick Hensen's node and Xenopus dorsal lip (Spemann's organizer), which can induce secondary body axes when grafted into host embryos (reviewed in refs 5 and 6). Our findings suggest that this gene, named nodal, encodes a signalling molecule essential for mesoderm formation and subsequent organization of axial structures in early mouse development. C1 NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. VANDERBILT UNIV,MED CTR,SCH MED,DEPT CELL BIOL,NASHVILLE,TN 37232. RI Kuehn, Michael/A-4573-2014 OI Kuehn, Michael/0000-0002-7703-9160 NR 22 TC 469 Z9 485 U1 3 U2 13 PU MACMILLAN MAGAZINES LTD PI LONDON PA PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW SN 0028-0836 J9 NATURE JI Nature PD FEB 11 PY 1993 VL 361 IS 6412 BP 543 EP 547 DI 10.1038/361543a0 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KL714 UT WOS:A1993KL71400063 PM 8429908 ER PT J AU FAUCI, AS AF FAUCI, AS TI CD4+ T-LYMPHOCYTOPENIA WITHOUT HIV-INFECTION - NO LIGHTS, NO CAMERA, JUST FACTS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material RP FAUCI, AS (reprint author), NIH,BETHESDA,MD 20892, USA. NR 9 TC 69 Z9 69 U1 0 U2 1 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD FEB 11 PY 1993 VL 328 IS 6 BP 429 EP 431 DI 10.1056/NEJM199302113280610 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA KL584 UT WOS:A1993KL58400010 PM 8093637 ER PT J AU VERMUND, SH HOOVER, DR CHEN, K AF VERMUND, SH HOOVER, DR CHEN, K TI CD4+ COUNTS IN SERONEGATIVE HOMOSEXUAL MEN SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter ID MULTICENTER AIDS COHORT C1 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,BALTIMORE,MD 21205. RP VERMUND, SH (reprint author), NIAID,BETHESDA,MD 20892, USA. OI Vermund, Sten/0000-0001-7289-8698 NR 5 TC 22 Z9 22 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD FEB 11 PY 1993 VL 328 IS 6 BP 442 EP 442 DI 10.1056/NEJM199302113280615 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA KL584 UT WOS:A1993KL58400015 PM 8093639 ER PT J AU NELSON, PS PAPAS, TS SCHWEINFEST, CW AF NELSON, PS PAPAS, TS SCHWEINFEST, CW TI RESTRICTION ENDONUCLEASE CLEAVAGE OF 5-METHYL-DEOXYCYTOSINE HEMIMETHYLATED DNA AT HIGH ENZYME-TO-SUBSTRATE RATIOS SO NUCLEIC ACIDS RESEARCH LA English DT Article ID CYTOSINE-METHYLATED DNA; EUKARYOTIC DNA; POLYMERASE; 5-METHYLCYTOSINE; SEQUENCES; ECORI; SITE AB We have investigated the ability of a large number of restriction enzymes to digest non-canonically hemimethylated DNA at high enzyme-to-substrate ratios. A single-stranded unmethylated phagemid was used as a template to complete synthesis of the second strand using 5-methyl-dCTP to substitute for all the deoxycytosine residues. A fragment of this double-stranded hemimethylated DNA which contains the multiple cloning site region was used as a substrate. For all the enzymes tested, at least some degree of protection from digestion is observed. Sites completely protected from digestion by their cognate enzymes are SaII, BstXI, SacI, SacII, SmaI, SstI, XhoI, PstI, HinfI, BamHI and AccI. Sites partially protected from digestion by their cognate enzymes are XbaI, HindIII, KpnI, SpeI, ClaI, EcoRI and PvuII. Knowledge of the sensitivity of commonly used restriction enzymes to hemimethylated substrates is useful for several applications, which will be discussed. C1 NCI,MOLEC ONCOL LAB,FREDERICK,MD 21702. NR 20 TC 24 Z9 26 U1 0 U2 3 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 11 PY 1993 VL 21 IS 3 BP 681 EP 686 DI 10.1093/nar/21.3.681 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KN345 UT WOS:A1993KN34500044 PM 8441677 ER PT J AU BORK, P KOONIN, EV AF BORK, P KOONIN, EV TI AN EXPANDING FAMILY OF HELICASES WITHIN THE DEAD/H SUPERFAMILY SO NUCLEIC ACIDS RESEARCH LA English DT Note ID ALIGNMENT C1 NIH,NATL CTR BIOTECHNOL INFORMAT,NATL LIB MED,BLDG 38A,8600 ROCKVILLE PIKE,BETHESDA,MD 20894. MAX DELBRUECK CTR MOLEC MED,W-1115 BERLIN,GERMANY. RI Bork, Peer/F-1813-2013 OI Bork, Peer/0000-0002-2627-833X NR 16 TC 113 Z9 115 U1 0 U2 5 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD FEB 11 PY 1993 VL 21 IS 3 BP 751 EP 752 DI 10.1093/nar/21.3.751 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KN345 UT WOS:A1993KN34500061 PM 8382805 ER PT J AU BERENDES, HW FORMAN, MR CNATTINGIUS, S AF BERENDES, HW FORMAN, MR CNATTINGIUS, S TI DOES DELAYED CHILDBEARING INCREASE RISK - REPLY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter C1 UNIV HOSP UPPSALA,UPPSALA,SWEDEN. RP BERENDES, HW (reprint author), NIH,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 10 PY 1993 VL 269 IS 6 BP 746 EP 747 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA KK544 UT WOS:A1993KK54400020 ER PT J AU RICHARDSON, GE VENZON, DJ PHELPS, R EDISON, M BROWN, M FRAME, JN IHDE, DC JOHNSON, BE AF RICHARDSON, GE VENZON, DJ PHELPS, R EDISON, M BROWN, M FRAME, JN IHDE, DC JOHNSON, BE TI APPLICATION OF AN ALGORITHM FOR STAGING SMALL-CELL LUNG-CANCER CAN SAVE 1/3 OF THE INITIAL EVALUATION COSTS SO ARCHIVES OF INTERNAL MEDICINE LA English DT Article ID BONE-MARROW TRANSPLANTATION; COMBINED MODALITY THERAPY; LEUKEMIA GROUP-B; BRONCHOGENIC-CARCINOMA; PROGNOSTIC FACTORS; COMBINATION CHEMOTHERAPY; COMPUTED-TOMOGRAPHY; RANDOMIZED TRIAL; ANAPLASTIC CARCINOMA; RADIATION-THERAPY AB Objective: Design of a cost-effective algorithm for staging disease in patients with small-cell lung cancer. Designs: An algorithm was constructed by analyzing all permutations of a sequence of procedures required to stage disease in patients with small-cell lung cancer. Procedural costs were determined, and the model was applied to the small-cell lung cancer patient population treated at the National Cancer Institute, Bethesda, Md, from 1973 to 1989. The final algorithm was derived from the permutation with the lowest cost per accurately staged patient. Setting: A single government institute, the National Cancer Institute. Patients: Four hundred fifty-one patients with previously untreated, consecutive histologically documented small-cell lung cancer entered into therapeutic protocols at the National Cancer Institute from April 1973 through July 1989. Data were obtained from small-cell lung cancer protocol databases and patients' medical records. Main Outcome Measures The cost per patient of each sequence of staging procedures when applied to the patient population. Results: The least expensive sequence of procedures saved $1418 per patient when compared with application of a standard set of staging procedures to all patients. The major factor in reducing costs was the concept of stopping the staging procedures after a site of distant metastatic disease had been identified. Conclusions: An algorithm consisting of a set of sequential staging procedures can accurately stage disease in patients with small-cell lung cancer and save more than one third of the costs of an inclusive standard set of staging procedures. C1 NCI, USN,MED ONCOL BRANCH,NATL NAVAL MED CTR,BLDG 8, ROOM 5105, BETHESDA, MD 20889 USA. NCI, BIOSTAT & DATA MANAGEMENT SECT, BETHESDA, MD 20892 USA. NCI, CLIN ONCOL PROGRAM, BETHESDA, MD 20892 USA. NCI, DIV CANC PREVENT & CONTROL, APPL RES BRANCH, BETHESDA, MD 20892 USA. UNIFORMED SERV UNIV HLTH SCI, DEPT MED, BETHESDA, MD 20814 USA. NATL NAVAL MED CTR, BETHESDA, MD 20814 USA. RI Venzon, David/B-3078-2008 NR 75 TC 27 Z9 27 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9926 J9 ARCH INTERN MED JI Arch. Intern. Med. PD FEB 8 PY 1993 VL 153 IS 3 BP 329 EP 337 DI 10.1001/archinte.153.3.329 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA KK886 UT WOS:A1993KK88600007 PM 8280192 ER PT J AU YOO, SH LIM, DJ AF YOO, SH LIM, DJ TI UBIQUITOUS PRESENCE OF CHROMOGRANIN-A IN THE INNER-EAR OF GUINEA-PIG SO FEBS LETTERS LA English DT Article DE CHROMOGRANIN-A; CHROMOGRANIN-A ANTIBODY; THE ORGAN OF CORTI; INNER EAR ID SECRETORY PROTEIN-I; CONFORMATIONAL CHANGE; SECRETOGRANIN-II; ADRENAL-MEDULLA; MESSENGER-RNA; CALCIUM; RAT; AGGREGATION; CHROMAFFIN; VESICLES AB Chromogranin A (CGA), which is supposed to be responsible for the calcium storage of secretory vesicles and is also considered to be a marker protein of neurons and endocrine cells, has been found in a variety of organs and tissues. In the present study, soluble proteins from the organ of Corti, saccule, crista, utricle, tectorial membrane, stria vascularis, and the spiral ligament from the inner ear of guinea pig were extracted, and probed with both polyclonal and monoclonal CGA antibodies to determine the presence of CGA. A 75 kDa protein reactve to both antibodies was found in the organ of Corti, saccule, crista, utricle, stria vascularis, and the spiral ligament, suggesting the widespread presence of CGA in the inner ear. RP YOO, SH (reprint author), NATL INST DEAFNESS & COMMUN DISORDERS,CELLULAR BIOL LAB,BLDG 36,ROOM 5D-15,BETHESDA,MD 20892, USA. NR 25 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD FEB 8 PY 1993 VL 317 IS 1-2 BP 113 EP 117 DI 10.1016/0014-5793(93)81503-R PG 5 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA KL015 UT WOS:A1993KL01500025 PM 8428619 ER PT J AU DENG, YP KANG, HC BENNINK, J YEWDELL, J AF DENG, YP KANG, HC BENNINK, J YEWDELL, J TI CHARACTERIZATION OF BODIPI AND BODIPY-DERIVATIVE-CONJUGATED-BREFELDINA SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 MOLEC PROBES INC,EUGENE,OR 97402. NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 23 EP 23 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600076 ER PT J AU STANG, E LONG, E BAKKE, O AF STANG, E LONG, E BAKKE, O TI INTRACELLULAR-LOCALIZATION OF MHC CLASS-II MOLECULES AND ASSOCIATED INVARIANT CHAIN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV OSLO,DEPT BIOL,MOLEC CELL BIOL UNIT,OSLO 3,NORWAY. NIH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 29 EP 29 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600101 ER PT J AU SHEARS, SB EVANS, WH AF SHEARS, SB EVANS, WH TI COMPARTMENTALIZATION AND SIGNAL TRANSDUCTION .1. BASOLATERAL-APICAL DISTRIBUTION OF INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE IN EPITHELIAL-CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 32 EP 32 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600112 ER PT J AU ALI, N CRAXTON, A SHEARS, SB AF ALI, N CRAXTON, A SHEARS, SB TI COMPARTMENTALIZATION AND SIGNAL TRANSDUCTION .2. HEPATIC INOSITOL 1,3,4,5 TETRAKISPHOSPHATE 3-PHOSPHATASE IS SEQUESTERED INSIDE ENDOPLASMIC-RETICULUM SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 33 EP 33 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600116 ER PT J AU STREBEL, K CHEN, MY MALDARELLI, F MARTIN, M WILLEY, R AF STREBEL, K CHEN, MY MALDARELLI, F MARTIN, M WILLEY, R TI THE HIV-1 VPU PROTEIN INDUCES RAPID DEGRADATION OF CD4 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,MOLEC MICROBIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 33 EP 33 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600115 ER PT J AU SETTE, A CEMAN, S KUBO, RT SAKAGUCHI, K APPELLA, E HUNT, DF DAVIS, TA MICHEL, H SHABANOWITZ, J RUDERSDORF, R GREY, HM DEMARS, R AF SETTE, A CEMAN, S KUBO, RT SAKAGUCHI, K APPELLA, E HUNT, DF DAVIS, TA MICHEL, H SHABANOWITZ, J RUDERSDORF, R GREY, HM DEMARS, R TI FINE SPECIFICITY OF CLASS-II BINDING OF PEPTIDES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 CYTEL,SAN DIEGO,CA. NCI,BETHESDA,MD 20892. UNIV WISCONSIN,GENET LAB,MADISON,WI 53706. UNIV VIRGINIA,DEPT CHEM,CHARLOTTESVILLE,VA 22903. RI Hunt, Donald/I-6936-2012 OI Hunt, Donald/0000-0003-2815-6368 NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 48 EP 48 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600166 ER PT J AU GERMAIN, RN BAKKE, O BONNEY, E CASTELLINO, F LAYET, C RINKER, AG SADEGHNASSERI, S ROMAGNOLI, P AF GERMAIN, RN BAKKE, O BONNEY, E CASTELLINO, F LAYET, C RINKER, AG SADEGHNASSERI, S ROMAGNOLI, P TI REGULATION OF MHC CLASS-II INTRACELLULAR FOLDING AND TRANSPORT BY INVARIANT CHAIN AND PEPTIDE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,LYMPHOCYTE BIOL SECT,BETHESDA,MD 20892. UNIV OSLO,DEPT BIOL,N-0316 OSLO,NORWAY. RI Romagnoli, Paola/K-2237-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 49 EP 49 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600170 ER PT J AU BEIER, DC COX, JH CRESSWELL, P ENGELHARD, VH AF BEIER, DC COX, JH CRESSWELL, P ENGELHARD, VH TI DEFINING THE INTERACTION BETWEEN HLA-A2.1 AND THE ADENOVIRUS E3/19K PROTEIN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV VIRGINIA,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,BEIRNE CARTER CTR IMMUNOL RES,CHARLOTTESVILLE,VA 22908. NIAID,BETHESDA,MD 20892. YALE UNIV,SCH MED,HOWARD HUGHES MED INST,IMMUNOBIOL SECT,NEW HAVEN,CT 06517. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 53 EP 53 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600180 ER PT J AU BIDDISON, WE GAMMON, MC HARRER, T WALKER, BD UTZ, U ZWEERINK, HJ AF BIDDISON, WE GAMMON, MC HARRER, T WALKER, BD UTZ, U ZWEERINK, HJ TI PRESENTATION OF ENDOGENOUS PEPTIDES TO MHC CLASS-I RESTRICTED CTL IN TRANSPORT DELETION MUTANT T2 CELLS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 MERCK RES LABS,RAHWAY,NJ. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 53 EP 53 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600182 ER PT J AU COLIGAN, JE HULL, LK DIBRINO, M UTZ, U BIDDISON, WE PARKER, KC AF COLIGAN, JE HULL, LK DIBRINO, M UTZ, U BIDDISON, WE PARKER, KC TI SEQUENCE MOTIFS IMPORTANT FOR PEPTIDE BINDING TO HUMAN MHC CLASS-I MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892. NINCDS,MOLEC IMMUNOL SECT,NEUROIMMUNOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 55 EP 55 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600189 ER PT J AU DAY, PM BENNINK, J LUKSZO, J YEWDELL, J AF DAY, PM BENNINK, J LUKSZO, J YEWDELL, J TI INTRACELLULAR ASSOCIATION OF A SYNTHETIC PEPTIDE WITH A MHC CLASS-I MOLECULE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,VIRAL DIS LAB,VIRAL IMMUNOL SECT,BETHESDA,MD 20892. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 55 EP 55 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600190 ER PT J AU LAPHAM, CK BACIK, I YEWDELL, JW KANE, K BENNINK, JR AF LAPHAM, CK BACIK, I YEWDELL, JW KANE, K BENNINK, JR TI CLASS-I MHC MOLECULES RETAINED IN THE ENDOPLASMIC-RETICULUM BIND TO NATURALLY PROCESSED ANTIGENIC PEPTIDES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 58 EP 58 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600201 ER PT J AU LONG, EO MALNATI, MS LAVAUTE, T CEMAN, S DEMARS, R AF LONG, EO MALNATI, MS LAVAUTE, T CEMAN, S DEMARS, R TI PRESENTATION OF ENDOGENOUS CYTOSOLIC ANTIGEN BY CLASS-II MHC MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. UNIV WISCONSIN,DEPT GENET,MADISON,WI 53706. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 59 EP 59 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600205 ER PT J AU RIBAUDO, RK MARGULIES, DH AF RIBAUDO, RK MARGULIES, DH TI POLYMORPHISM AT POSITION 9 OF THE MHC CLASS-I HEAVY-CHAIN CONTROLS THE STABILITY OF ASSOCIATION WITH BETA-2-MICROGLOBULIN AS EVALUATED IN A CELL FREE PROTEIN ASSEMBLY SYSTEM SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,MOLEC BIOL SECT,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 61 EP 61 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600211 ER PT J AU KARP, DR JENKINS, RN LONG, EO AF KARP, DR JENKINS, RN LONG, EO TI STAPHYLOCOCCAL ENTEROTOXINS AND CLASS-II-ASSOCIATED INVARIANT CHAIN BIND TO DISTINCT SITES ON HLA-DR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UT,SW MED CTR,ARTHRITIS RES CTR,DALLAS,TX 75235. NIH,IMMUNOGENET LAB,BETHESDA,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 69 EP 69 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600242 ER PT J AU KOVATS, S BLUM, JS SETTE, A SAKAGUCHI, K APPELLA, E KWOK, W NEPOM, GT AF KOVATS, S BLUM, JS SETTE, A SAKAGUCHI, K APPELLA, E KWOK, W NEPOM, GT TI ALLELIC COMPETITION IN PEPTIDE/HLA CLASS-II ALPHA-BETA INTERACTIONS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 VIRGINIA MASON RES CTR,IMMUNOL PROGRAM,SEATTLE,WA 98101. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 69 EP 69 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600243 ER PT J AU ROCHE, PA TELETSKI, CL BAKKE, O LONG, EO AF ROCHE, PA TELETSKI, CL BAKKE, O LONG, EO TI THE CYTOPLASMIC TAIL OF THE INVARIAN CHAIN MEDIATES RAPID ENDOCYTOSIS OF HLA-DR SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOGENET LAB,ROCKVILLE,MD 20852. UNIV OSLO,DEPT BIOL,OSLO 3,NORWAY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 73 EP 73 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600260 ER PT J AU ROMAGNOLI, P CASTELLINO, F GERMAIN, RN AF ROMAGNOLI, P CASTELLINO, F GERMAIN, RN TI INVARIANT CHAIN MODIFIES ENDOSOME MORPHOLOGY AND TRANS-ENDOSOMAL TRANSPORT SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Romagnoli, Paola/K-2237-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 73 EP 73 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600259 ER PT J AU SADEGHNASSERI, S STERN, L WILEY, D GERMAIN, R AF SADEGHNASSERI, S STERN, L WILEY, D GERMAIN, R TI DISTINCT KINETIC STATES OF MHC CLASS II-PEPTIDE INTERACTION CORRELATE WITH DISTINCT CLASS-II STRUCTURES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,IMMUNOL LAB,BETHESDA,MD 20892. HARVARD UNIV,DEPT BIOCHEM,CAMBRIDGE,MA 02138. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 73 EP 73 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600261 ER PT J AU BERZOFSKY, JA SHIRAI, M VACCHIO, MS HODES, RJ AF BERZOFSKY, JA SHIRAI, M VACCHIO, MS HODES, RJ TI PREFERENTIAL V-BETA USAGE BY CYTOTOXIC T-CELLS WITH THE UNUSUAL PHENOTYPE OF CROSS-REACTIVE RECOGNITION OF 2 HIV-1 EPITOPES AND DEGENERACY OF PRESENTATION BY 3 CLASS-I MHC MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,MOLEC IMMUNOGENET & VACCINE RES SECT,METAB BRANCH,BETHESDA,MD 20892. NCI,EXPTL IMMUNOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 77 EP 77 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600272 ER PT J AU LEE, L MCHUGH, L RIBAUDO, RK KOZLOWSKI, S MARGULIES, DH MAGE, MG AF LEE, L MCHUGH, L RIBAUDO, RK KOZLOWSKI, S MARGULIES, DH MAGE, MG TI CELL-SURFACE EXPRESSION AND ANTIGEN PRESENTATION BY A RECOMBINANT SINGLE CHAIN CLASS-I MHC MOLECULE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 80 EP 80 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600286 ER PT J AU MAGE, MG CORR, M LEE, L MCHUGH, L RIBAUDO, RK KOZLOWSKI, S MARGULIES, DH AF MAGE, MG CORR, M LEE, L MCHUGH, L RIBAUDO, RK KOZLOWSKI, S MARGULIES, DH TI ENDOGENOUS PEPTIDES AND PEPTIDE-LOADING OF RECOMBINANT SOLUBLE SINGLE CHAIN CLASS-I MHC MOLECULES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NIAID,IMMUNOL LAB,BETHESDA,MD 20892. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 80 EP 80 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600287 ER PT J AU RESTIFO, NP BACIK, I BENNINK, JR ROSENBERG, SA YEWDELL, JW AF RESTIFO, NP BACIK, I BENNINK, JR ROSENBERG, SA YEWDELL, JW TI MAKING ANTIGEN PROCESSING SUPERFLUOUS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. RI Restifo, Nicholas/A-5713-2008; yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 87 EP 87 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600315 ER PT J AU OTTENHOFF, THM ENGEL, I KLAUSNER, RD AF OTTENHOFF, THM ENGEL, I KLAUSNER, RD TI HIGH-EFFICIENCY EXPRESSION AND SOLUBILIZATION OF FUNCTIONAL T-CELL ANTIGEN RECEPTOR HETERODIMERS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NICHHD,CELL BIOL & MET BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 91 EP 91 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600329 ER PT J AU WILLIAMS, SA CHANTEAU, S MIN, Z AF WILLIAMS, SA CHANTEAU, S MIN, Z TI PCR DETECTION OF WUCHERERIA-BANCROFTI DNA IN SERUM, BLOOD AND MOSQUITOS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 SMITH COLL,CLARK SCI CTR,NORTHAMPTON,MA 01063. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. INST TERR RECH MED LOUIS MALARDE,PAPEETE,FR POLYNESIA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 121 EP 121 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600431 ER PT J AU KLAR, A THON, G SINGH, J BONADUCE, M AF KLAR, A THON, G SINGH, J BONADUCE, M TI DIRECTIONALITY AND CELL FATE DETERMINATION CONTROLS IN FISSION YEAST MATING-TYPE DETERMINATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,EUKARYOT GENE EXPRESS LAB,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 126 EP 126 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600441 ER PT J AU RICHARDSON, DL LOOMIS, WF KIMMEL, AR AF RICHARDSON, DL LOOMIS, WF KIMMEL, AR TI EXPRESSION OF THE SPIA GENE REVEALS A SPATIAL GRADIENT DURING DICTYOSTELIUM SPORULATION SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892. UCSD,DEPT BIOL,LA JOLLA,CA 92093. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 144 EP 144 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600504 ER PT J AU CRAIGIE, R BUSHMAN, FD ENGELMAN, A HICKMAN, A AF CRAIGIE, R BUSHMAN, FD ENGELMAN, A HICKMAN, A TI FUNCTIONAL-ORGANIZATION OF HIV INTEGRASE PROTEIN - A 136 AMINO-ACID DOMAIN IS NECESSARY AND SUFFICIENT FOR CATALYSIS OF PHOSPHORYLTRANSFER SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 146 EP 146 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600506 ER PT J AU GU, L HUANG, SM SANDER, M AF GU, L HUANG, SM SANDER, M TI SYNTHESIS OF DROSOPHILA RRP1 IN AN APURINIC ENDONUCLEASE DEFICIENT STRAIN OF ESCHERICHIA-COLI - COMPLEMENTATION OF REPAIR DEFICIENCY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,GENET LAB D304,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 163 EP 163 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600560 ER PT J AU SANDER, M NUGENT, M HUANG, SM AF SANDER, M NUGENT, M HUANG, SM TI CHARACTERIZATION OF THE APURINIC ENDONUCLEASE AND EXONUCLEASE ACTIVITIES OF DROSOPHILA RRP1 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,GENET LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 167 EP 167 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600576 ER PT J AU SAMUELS, DS GARON, CF AF SAMUELS, DS GARON, CF TI COUMERMYCIN-A1 RESISTANCE IN THE LYME-DISEASE AGENT, BORRELIA-BURGDORFERI SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIAID,ROCKY MT LABS,VECTORS & PATHOGENS LAB,HAMILTON,MT 59840. RI Samuels, D Scott/B-7549-2012 OI Samuels, D Scott/0000-0001-8352-7593 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 171 EP 171 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600590 ER PT J AU BURGESS, K HO, KK LAKE, JR MALIN, DH PAYZA, K AF BURGESS, K HO, KK LAKE, JR MALIN, DH PAYZA, K TI ASYMMETRIC SYNTHESES OF 2,3-METHANOLOGS, SOME BIOACTIVITIES OF PEPTIDOMIMETICS FORMED FROM THESE, AND THEIR INFLUENCE ON SECONDARY STRUCTURE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 TEXAS A&M UNIV SYST, COLL STN, TX 77843 USA. UNIV HOUSTON CLEAR LAKE, HOUSTON, TX 77058 USA. NIMH, WASHINGTON, DC 20032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 197 EP 197 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600670 ER PT J AU LONDON, RE PERLMAN, ME DAVIS, DG AF LONDON, RE PERLMAN, ME DAVIS, DG TI DETERMINATION OF LIGAND-EXCHANGE RATES AND THEIR EFFECT ON TRANSFERRED NOE MEASUREMENTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 1 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 211 EP 211 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600709 ER PT J AU DRISCOLL, PC SAKAGUCHI, K OMISCHINSKI, JG APPELLA, E BONIFACE, JJ ALTMAN, J DAVIS, MM AF DRISCOLL, PC SAKAGUCHI, K OMISCHINSKI, JG APPELLA, E BONIFACE, JJ ALTMAN, J DAVIS, MM TI NUCLEAR-MAGNETIC-RESONANCE STUDIES OF A SOLUBLE CLASS-II MHC PEPTIDE COMPLEX SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND. NCI,CELL BIOL LAB,BETHESDA,MD 20892. STANFORD UNIV,HOWARD HUGHES MED INST,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305. RI Driscoll, Paul/B-8007-2010 OI Driscoll, Paul/0000-0002-4124-6950 NR 2 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 215 EP 215 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600721 ER PT J AU JERNIGAN, RL COVELL, DG YOUNG, L AF JERNIGAN, RL COVELL, DG YOUNG, L TI PEPTIDE BINDING-SITES ON PROTEINS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NCI,MATH BIOL LAB,BETHESDA,MD 20892. RI Jernigan, Robert/A-5421-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 224 EP 224 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600759 ER PT J AU NUSSINOV, R NOREL, R FISCHER, D WOLFSON, HJ AF NUSSINOV, R NOREL, R FISCHER, D WOLFSON, HJ TI MOLECULAR-SURFACE RECOGNITION BY A COMPUTER VISION BASED TECHNIQUE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 PRI DYNACORP,NCI,FCRDC,MATH BIOL LAB,FREDERICK,MD 21702. TEL AVIV UNIV,SCH MED,IL-69978 TEL AVIV,ISRAEL. RI Wolfson, Haim/A-1837-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 225 EP 225 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600761 ER PT J AU BEDNAREK, MA PARKER, KC CUNNINGHAM, BR ZWEERINK, HJ COLIGAN, JE SPRINGER, JP AF BEDNAREK, MA PARKER, KC CUNNINGHAM, BR ZWEERINK, HJ COLIGAN, JE SPRINGER, JP TI INTERACTION OF HUMAN HISTOCOMPATIBILITY ANTIGEN HLA-A2 WITH SYNTHETIC PEPTIDES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 MERCK RES LABS,RAHWAY,NJ 07065. NIAID,BIOL RES BR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 229 EP 229 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600779 ER PT J AU ANGLISTER, J BAX, A DELAGLIO, F GRZESIEK, S VUISTER, GW AF ANGLISTER, J BAX, A DELAGLIO, F GRZESIEK, S VUISTER, GW TI RECENT ADVANCES IN THE STUDY OF ISOTOPICALLY ENRICHED PROTEINS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 2 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 243 EP 243 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600821 ER PT J AU LONDON, RE PERLMAN, ME DAVIS, DG AF LONDON, RE PERLMAN, ME DAVIS, DG TI DETERMINATION OF LIGAND-EXCHANGE RATES AND THEIR EFFECT ON TRANSFERRED NOE MEASUREMENTS SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 249 EP 249 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600840 ER PT J AU ARCHER, SJ TORCHIA, DA AF ARCHER, SJ TORCHIA, DA TI TRANSFORMING GROWTH FACTOR-BETA-1 - STRUCTURAL STUDIES USING HETERONUCLEAR NMR-SPECTROSCOPY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 270 EP 270 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600919 ER PT J AU KALIA, YN BROCKLEHURST, SM HIPPS, DS APPELLA, E SAKAGUCHI, K PERHAM, RN AF KALIA, YN BROCKLEHURST, SM HIPPS, DS APPELLA, E SAKAGUCHI, K PERHAM, RN TI HIGH-RESOLUTION STRUCTURE OF A MINI PROTEIN SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 UNIV CAMBRIDGE,DEPT BIOCHEM,CAMBRIDGE CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1QW,ENGLAND. NCI,CELL BIOL LAB,BETHESDA,MD 20890. NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 276 EP 276 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600944 ER PT J AU POWERS, R GARRETT, DS MARCH, CJ FRIEDEN, EA GRONENBORN, AM CLORE, GM AF POWERS, R GARRETT, DS MARCH, CJ FRIEDEN, EA GRONENBORN, AM CLORE, GM TI THE HIGH-RESOLUTION 3-DIMENSIONAL SOLUTION STRUCTURE OF HUMAN INTERLEUKIN-4 BY MULTIDIMENSIONAL HETERONUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 282 EP 282 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46600968 ER PT J AU WERNER, MH CLORE, GM GRONENBORN, A NASH, HA AF WERNER, MH CLORE, GM GRONENBORN, A NASH, HA TI STRUCTURAL INVESTIGATION OF A PUTATIVE MINOR GROOVE BINDING-PROTEIN - ESCHERICHIA-COLI INTEGRATION HOST FACTOR BOUND TO A 30BP BINDING-SITE SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDKD,BETHESDA,MD 20895. NIMH,BETHESDA,MD 20895. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 303 EP 303 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601053 ER PT J AU GARRETT, DS POWERS, R LODI, P GRONENBORN, AM CLORE, GM AF GARRETT, DS POWERS, R LODI, P GRONENBORN, AM CLORE, GM TI AUTOMATED AND INTERACTIVE TOOLS FOR ASSIGNING 3D-NMR AND 4D-NMR DATA - APPLICATION TO INTERLEUKIN-4 AND HNRNP-A1 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 306 EP 306 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601061 ER PT J AU LABHARDT, AM KLAUS, W GRZESIEK, S HUNZIKER, W AF LABHARDT, AM KLAUS, W GRZESIEK, S HUNZIKER, W TI H-1, C-13 AND N-15 SEQUENTIAL BACKBONE ASSIGNMENTS AND SECONDARY STRUCTURE OF A CONSTRUCT OF CALBINDIN-D28K SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 F HOFFMANN LA ROCHE LTD,NEW TECHNOL,PHARMACEUT RES,BASEL,SWITZERLAND. NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 307 EP 307 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601066 ER PT J AU LODI, PJ GARRETT, DS GRONENBORN, AM CLORE, GM AF LODI, PJ GARRETT, DS GRONENBORN, AM CLORE, GM TI STRUCTURAL STUDIES ON THE RNA-BINDING DOMAIN OF THE HNRNP-A1 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 308 EP 308 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601069 ER PT J AU OMICHINSKI, JG CLORE, GM TRAINOR, C FELSENFELD, G STAHL, S GRONENBORN, AM AF OMICHINSKI, JG CLORE, GM TRAINOR, C FELSENFELD, G STAHL, S GRONENBORN, AM TI DNA-BINDING AND NMR-STUDIES WITH A SINGLE-FINGER PEPTIDE FROM THE ERYTHROID FACTOR GATA-1 SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NIDDK,MOLEC BIOL LAB,BETHESDA,MD 20892. NIH,PROT EXPRESS LAB,BETHESDA,MD 20892. RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 308 EP 308 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601072 ER PT J AU PELTON, JG TORCHIA, DA MEADOW, ND ROSEMAN, S AF PELTON, JG TORCHIA, DA MEADOW, ND ROSEMAN, S TI TAUTOMERIC STATES OF THE HISTIDINES OF PHOSPHORYLATED AND UNPHOSPHORYLATED IIIGLC FROM ESCHERICHIA-COLI USING 2D-HETERONUCLEAR NMR TECHNIQUES SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Meeting Abstract C1 NIDR,BONE RES LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,MCCOLLUM PRATT INST,BALTIMORE,MD 21218. NR 0 TC 0 Z9 0 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD FEB 8 PY 1993 SU 17C BP 309 EP 309 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KN466 UT WOS:A1993KN46601073 ER PT J AU HENGGE, UR BROCKMEYER, NH RASSHOFER, R GOOS, M AF HENGGE, UR BROCKMEYER, NH RASSHOFER, R GOOS, M TI FATAL HEPATIC-FAILURE WITH LIPOSOMAL DOXORUBICIN SO LANCET LA English DT Letter ID CHEMOTHERAPY; WITHDRAWAL; INFECTION; THERAPY C1 UNIV ESSEN GESAMTHSCH,DEPT DERMATOL,W-4300 ESSEN 1,GERMANY. UNIV ESSEN GESAMTHSCH,DEPT VENEROL,W-4300 ESSEN 1,GERMANY. UNIV ESSEN GESAMTHSCH,DEPT ALLERGOL & VIROL,W-4300 ESSEN 1,GERMANY. RP HENGGE, UR (reprint author), NCI,DERMATOL BRANCH,BLDG 10,12N260,BETHESDA,MD 20892, USA. NR 6 TC 20 Z9 20 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD FEB 6 PY 1993 VL 341 IS 8841 BP 383 EP 384 DI 10.1016/0140-6736(93)90198-P PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA KK939 UT WOS:A1993KK93900058 PM 8094161 ER PT J AU SORIANO, V GONZALEZLAHOZ, J LEONMONZON, M AF SORIANO, V GONZALEZLAHOZ, J LEONMONZON, M TI RETROVIRUSES AND AUTOIMMUNE-DISEASES SO MEDICINA CLINICA LA Spanish DT Review ID ACQUIRED IMMUNODEFICIENCY SYNDROME; I-ASSOCIATED MYELOPATHY; T-CELL LEUKEMIA; VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; SYSTEMIC LUPUS-ERYTHEMATOSUS; IMMUNE-DEFICIENCY SYNDROME; MULTIPLE-SCLEROSIS PATIENTS; LARGE GRANULAR LYMPHOCYTES; HTLV-I C1 INST SALUD CARLOS 3,CTR INVEST CLIN,SERV ENFERMEDADES INFECCIOSAS,MADRID,SPAIN. NIH,CTR CLIN,MED NEUROL BRANCH,BETHESDA,MD 20892. NR 147 TC 2 Z9 2 U1 0 U2 0 PU EDICIONES DOYMA S/A PI BARCELONA PA TRAV DE GRACIA 17-21, 08021 BARCELONA, SPAIN SN 0025-7753 J9 MED CLIN-BARCELONA JI Med. Clin. PD FEB 6 PY 1993 VL 100 IS 5 BP 181 EP 186 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA KM266 UT WOS:A1993KM26600008 PM 8450698 ER PT J AU OZAWA, K YAMADA, K KAZANIETZ, MG BLUMBERG, PM BEAVEN, MA AF OZAWA, K YAMADA, K KAZANIETZ, MG BLUMBERG, PM BEAVEN, MA TI DIFFERENT ISOZYMES OF PROTEIN-KINASE-C MEDIATE FEEDBACK INHIBITION OF PHOSPHOLIPASE-C AND STIMULATORY SIGNALS FOR EXOCYTOSIS IN RAT RBL-2H3 CELLS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID PHORBOL ESTER BINDING; SYNERGISTIC SIGNALS; DOWN-REGULATION; 2H3 CELLS; EXPRESSION; RECEPTOR; FAMILY; SECRETION; EPSILON; RELEASE AB Previous studies indicated that rat basophilic RBL-2H3 cells contained the Ca2+-dependent alpha and beta and the Ca2+-independent delta, epsilon, and zeta isoforms of protein kinase C (PKC); of these, PKCbeta and delta were the most potent transducers of signals for exocytosis in antigen-stimulated permeabilized cells. Exocytosis, nevertheless, was still dependent on an elevated free Ca2+. (Ozawa, K., Szallasi, Z., Kazanietz, M. G., Blumberg, P. M., Mischak, H., Mushinski, J. F., and Beaven, M. A. (1993) J. Biol. Chem. 268, 1749-1756). We now demonstrate that PKCalpha and epsilon, exclusively, inhibit antigen-induced hydrolysis of inositol phospholipids in the same permeabilized RBL-2H3 cells. Unlike secretion, the inhibitory actions occurred at a basal concentration (0.1 muM) of free Ca2+. The inhibitory actions of the two isozymes were potentiated by 20 nM phorbol 12-myristate 13-acetate. As indicated by the effects of the phorbol ester, the probable mechanism was reduced tyrosine phosphorylation of phospholipase C gamma1. The negative regulation of phospholipase C was apparent in intact cells, because the PKC inhibitor Ro31-7549 or down-regulation of PKC with phorbol ester enhanced antigen-induced hydrolysis of inositol phospholipids. The concentrations of the various isozymes of PKC in RBL-2H3 cells, as estimated by immunoblotting studies, were sufficient for promotion of exocytosis (i.e. beta and delta) and inhibition of phospholipid hydrolysis (i.e. alpha and epsilon). C1 NHLBI, CHEM PHARMACOL LAB, RM 8N114, BLDG 10, BETHESDA, MD 20892 USA. NCI, CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB, BETHESDA, MD 20892 USA. NR 31 TC 161 Z9 161 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2280 EP 2283 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500004 PM 8381401 ER PT J AU YOSHIMURA, K ROSENFELD, MA SETH, P CRYSTAL, RG AF YOSHIMURA, K ROSENFELD, MA SETH, P CRYSTAL, RG TI ADENOVIRUS-MEDIATED AUGMENTATION OF CELL TRANSFECTION WITH UNMODIFIED PLASMID VECTORS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Note ID EPIDERMAL GROWTH-FACTOR; MEMBRANE-PERMEABILITY; KB-CELLS; PH; GENE; DNA; MACROMOLECULES; ENHANCEMENT; EXPRESSION; INFECTION AB The present study demonstrates that the human adenovirus (Ad) can augment transfer and expression of a gene within plasmid DNA unmodified by nonspecific linkers or by linker-ligand complexes. Following the transfection of COS-7 cells with pRSVL, a luciferase expression plasmid vector directed by the Rous sarcoma virus-long terminal repeat promoter, luciferase activity in the target cells was 10(3)- to 10(4)-fold higher when the cells were also infected with Ad-CFTR, a replication-deficient recombinant Ad containing human cystic fibrosis transmembrane conductance regulator (CFTR) cDNA. The enhancement of luciferase gene expression in COS-7 cells was also observed with Ad-dl312 (a replication-deficient E1a deletion mutant Ad with no exogenous gene) and wild type Ad5. The efficiency of cell transfection with pRSVL in the presence of an Ad was achieved in a dose-dependent fashion with progressively higher luciferase activity in cells infected by increasing amounts of Ad-CFTR, Ad-dl312, or Ad5. The augmentation by Ad-CFTR of the transfer and expression of the luciferase gene in cells was similar to that of another transfection reagent, cationic liposomes. Further, when Ad-CFTR and liposomes were used in combination, 4- to 100-fold more efficient expression of the luciferase gene was achieved than with Ad-CFTR or liposomes alone. When COS-7, HeLa, and CV-1 cells were evaluated in parallel in the presence or absence of liposomes, Ad-mediated enhancement of luciferase activity was observed in all cell lines. Thus, exposure of target cells to replication-deficient or competent human Ad will markedly augment transfer and expression of the genes within plasmid DNA in mammalian cells in vitro without modifying the plasmid with linkers or linker-ligand complexes, a strategy that should be useful for in vitro and in vivo gene transfer applications. C1 NHLBI,PULM BRANCH,BLDG 10,RM 6D03,BETHESDA,MD 20892. NR 30 TC 98 Z9 99 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2300 EP 2303 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500009 PM 8428904 ER PT J AU SVITIL, AL CASHEL, M ZYSKIND, JW AF SVITIL, AL CASHEL, M ZYSKIND, JW TI GUANOSINE TETRAPHOSPHATE INHIBITS PROTEIN-SYNTHESIS INVIVO - A POSSIBLE PROTECTIVE MECHANISM FOR STARVATION STRESS IN ESCHERICHIA-COLI SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID UNCHARGED TRANSFER RNATRP; RIBOSOMAL-RNA; PPGPP; EXPRESSION; METHIONINE; TRANSPORT; MUTANTS AB Guanosine 3',5'-bispyrophosphate (ppGpp) accumulates in bacteria in response to either amino acid or energy source starvation. Here we demonstrate that levels of ppGpp similar to those induced by amino acid starvation inhibit the rate of protein synthesis by 84-91%. The intracellular concentration of ppGpp is manipulated in our studies by increasing the expression of a truncated relA gene encoding a smaller but catalytically active peptide with ppGpp synthetase activity. We find that the intracellular activity of the truncated RelA peptide is insensitive to chloramphenicol, unlike the product of the wild-type relA gene, ppGpp synthetase I. Previously, this same ppGpp expression system was used (Schreiber, G., Metzger, S., Aizenman, E., Roza, S., Cashel, M., and Glaser, G. (1991) J. Biol. Chem. 226, 3760-3767) to demonstrate that increasing the ppGpp concentration inhibits growth and ribosomal RNA transcription, and they found suggestive evidence for ppGpp inhibition of protein synthesis. We further investigated the effect of ppGpp on protein synthesis and find that ppGpp is a potent inhibitor of protein synthesis as well as glycerol accumulation but has no effect on transport of methionine, the amino acid used in measuring protein synthesis rates, or on uptake of alpha-methylglucoside, a non-metabolizable analogue of glucose. C1 SAN DIEGO STATE UNIV,DEPT BIOL,SAN DIEGO,CA 92182. NICHHD,MOLEC GENET LAB,BETHESDA,MD 20892. NR 29 TC 125 Z9 125 U1 2 U2 8 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2307 EP 2311 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500011 PM 8428905 ER PT J AU JOUNEAUX, C AUDIGIER, Y GOLDSMITH, P PECKER, F LOTERSZTAJN, S AF JOUNEAUX, C AUDIGIER, Y GOLDSMITH, P PECKER, F LOTERSZTAJN, S TI G(S) MEDIATES HORMONAL INHIBITION OF THE CALCIUM-PUMP IN LIVER PLASMA-MEMBRANES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GTP-BINDING PROTEIN; CARBOXYL-TERMINAL DECAPEPTIDE; BETA-GAMMA-SUBUNITS; CA-2+ PUMP; ADENYLATE-CYCLASE; ALPHA-SUBUNIT; IDENTIFICATION; RECEPTOR; GS; CALCITONIN AB We have reported that the calcium pump in liver plasma membranes is coupled to G(s) or a G(s)-like protein. However, we show here that isoproterenol, which activated adenylyl cyclase via G(s), had no effect on the calcium pump, while human calcitonin, human parathyroid hormone, and mini-glucagon, which inhibited this system, did not affect adenylyl cyclase activity. In order to determine the nature of the G protein coupled to the calcium pump, we used the RM antibody, raised against the carboxyl-terminal decapeptide of G(s)alpha, which antagonized adenylyl cyclase activation by isoproterenol or glucagon. The RM antibody specifically blocked calcium pump inhibition by mini-glucagon, calcitonin, or parathyroid hormone, while it did not affect guanosine 5'-O-(thiotriphosphate) inhibition. Its effect was mimicked the corresponding decapeptide RMHLRQYELL. The AS/7 antibody, reactive with G(t)alpha, G(i)1alpha, and G(i)2alpha, was ineffective. Complementation of liver plasma membranes with in vitro translated G(s)alpha-2, the large form of G(s)alpha, led to a 40% decrease in calcium pump activity, with a parallel 2-fold increase in adenylyl cyclase activity. In vitro translated G(i)1alpha did not affect the calcium pump activity, while it evoked a 40% inhibition of adenylyl cyclase activity. We conclude that a same G(s)alpha may be coupled either to the calcium pump or to adenylyl cyclase. However, G(s) is functionally specialized, since it does not ensure cross-talk between the two receptor-effector systems. These results point out the possible compartmentalization of G(s). C1 NIDDKD, MOLEC PATHOPHYSIOL BRANCH, BETHESDA, MD 20892 USA. CNRS, INSERM ENDOCRINOL, F-34094 MONTPELLIER, FRANCE. RP JOUNEAUX, C (reprint author), HOP HENRI MONDOR, INSERM, U99, F-94010 CRETEIL, FRANCE. NR 34 TC 28 Z9 28 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2368 EP 2372 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500021 PM 8428911 ER PT J AU LARMINAT, F ZHEN, WP BOHR, VA AF LARMINAT, F ZHEN, WP BOHR, VA TI GENE-SPECIFIC DNA-REPAIR OF INTERSTRAND CROSS-LINKS INDUCED BY CHEMOTHERAPEUTIC-AGENTS CAN BE PREFERENTIAL SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HAMSTER OVARY CELLS; DIHYDROFOLATE-REDUCTASE GENE; DHFR GENE; CIS-DIAMMINEDICHLOROPLATINUM(II); REMOVAL; ADDUCTS; RECOMBINATION; MONOADDUCTS; CISPLATIN; MECHANISM AB The gene-specific formation and repair of interstrand cross-links (ICL) were measured in the dihydrofolate reductase (DHFR) gene in hamster cells. Cells were treated with two different chemotherapeutic agents, nitrogen mustard and cisplatin, and the frequency of cross-links was quantified in the active gene and in a downstream, inactive region. About 5% of total lesions induced by these agents were ICL. Whereas the frequencies of cross-links formed were similar in the gene and in the noncoding region after cisplatin treatment, there were more nitrogen mustard-induced cross-links in the inactive region than in the active gene. At low levels of cross-linking, we found preferential DNA repair in the active gene as compared to the inactive region. At higher levels of cross-linking, there was no difference in repair rates between the gene and the noncoding region due to an increase in the repair efficiency in the inactive DNA. Implications of fine structural organization of cross-link repair are discussed. C1 NCI, DIV CANC TREATMENT, MOLEC PHARMACOL LAB, BETHESDA, MD 20892 USA. NR 35 TC 48 Z9 48 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2649 EP 2654 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500059 PM 8428941 ER PT J AU KATO, H FARIA, TN STANNARD, B ROBERTS, CT LEROITH, D AF KATO, H FARIA, TN STANNARD, B ROBERTS, CT LEROITH, D TI ROLE OF TYROSINE KINASE-ACTIVITY IN SIGNAL TRANSDUCTION BY THE INSULIN-LIKE GROWTH FACTOR-I (IGF-I) RECEPTOR - CHARACTERIZATION OF KINASE-DEFICIENT IGF-I RECEPTORS AND THE ACTION OF AN IGF-I-MIMETIC ANTIBODY (ALPHA-IR-3) SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MONOCLONAL-ANTIBODIES; ORNITHINE DECARBOXYLASE; MESSENGER-RNA; CELLS; PHOSPHORYLATION; OVEREXPRESSION; REPLACEMENT; BINDING; AUTOPHOSPHORYLATION; EXPRESSION AB The insulin-like growth factor-I (IGF-I) receptor is a member of a large family of transmembrane signal transducing molecules. The defining characteristic of this class of receptors is the intrinsic tyrosine kinase activity of the cytoplasmic domain. While it has been demonstrated that this tyrosine kinase activity is necessary for the action of a number of transmembrane tyrosine kinase receptors, no evidence of this type has been adduced to date with respect to the signaling requirement of the IGF-I receptor. We have now shown that stably transfected NIH-3T3 cell lines overexpressing human IGF-I receptors display increased responses to IGF-I and an IGF-I-mimetic antibody, alphaIR-3, in terms of short, intermediate, and long term actions initiated by activation of the IGF-I receptor. These include receptor autophosphorylation, activation of phosphatidylinositol-3-kinase and 2-deoxyglucose uptake, induction of ornithine decarboxylase gene expression, and stimulation of thymidine incorporation. In short term responses, the kinetics seen with alphaIR-3 were slower than those seen with IGF-I. These effects were severely decreased in clones expressing human IGF-I receptors in which the lysine residue in the ATP-binding site of the tyrosine kinase domain had been mutated to alanine or arginine. This was true for both IGF-I and alphaIR-3. These results indicate that, for all parameters tested, the tyrosine kinase activity of the IGF-I receptor is necessary for activation of the IGF-I-stimulated signal transduction cascade. Additionally, the effects of alphaIR-3 also require tyrosine kinase activity. C1 NIDDKD,DIABET BRANCH,BETHESDA,MD 20892. NR 43 TC 236 Z9 239 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2655 EP 2661 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500060 PM 7679099 ER PT J AU MARINI, JC LEWIS, MB WANG, Q CHEN, KJ ORRISON, BM AF MARINI, JC LEWIS, MB WANG, Q CHEN, KJ ORRISON, BM TI SERINE FOR GLYCINE SUBSTITUTIONS IN TYPE-I COLLAGEN IN 2 CASES OF TYPE-IV OSTEOGENESIS IMPERFECTA (OI) - ADDITIONAL EVIDENCE FOR A REGIONAL MODEL OF OI PATHOPHYSIOLOGY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRIPLE HELIX; POINT MUTATIONS; THERMAL-STABILITY; PROCOLLAGEN; CHAIN; GENE; COL1A1; SEQUENCES; POSITION; PROTEIN AB Serine for glycine substitutions in type I collagen have been described in seven cases of lethal type II osteosgenesis imperfecta (OI), and six cases of moderately thal OI. We describe here two cases of moderately severe type IV OI with serine substitutions at alpha1(I) Gly352 and alpha2(I) Gly922, respectively. In both cases, G A point mutations were detected by RNase A cleavage age of RNA/RNA and RNA/DNA hybrids. These cases extend the location for serine substitutions producing the moderately severe OI phenotype to the alpha2(I) chain and the amino-terminal end of the alpha1(I) chain. Their location supports a regional model of OI pathophysiology for serine substitutions. The proband with alpha2(I) Gly922 --> Ser has both normal and overmodified forms of both type I collagen chains. The overmodified form has delayed migration of all CNBr peptides. Helix thermal stability is decreased 4-degrees-C. The fibroblast collagen protein and RNA of her unaffected parents are normal. However, the father was demonstrated to be a mosaic carrier using leukocyte DNA. The fibroblasts of the proband whose serine substitution is at alpha1(I) Gly352 synthesize type I procollagen chains with delayed electrophoretic migration; normally migrating forms are difficult to detect. Only alpha1(I) CB 8 displayed delayed migration. Helix thermal stability is reduced 2-degrees-C. Parental genomic DNA was normal. RP MARINI, JC (reprint author), NICHHD,HUMAN GENET BRANCH,CONNECT TISSUE DISORDERS SECT,BLDG 10,RM 9S242,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 27 TC 52 Z9 54 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2667 EP 2673 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500062 PM 8094076 ER PT J AU STEINERT, PM PARRY, DAD AF STEINERT, PM PARRY, DAD TI THE CONSERVED H1 DOMAIN OF THE TYPE-II KERATIN-1 CHAIN PLAYS AN ESSENTIAL ROLE IN THE ALIGNMENT OF NEAREST NEIGHBOR MOLECULES IN MOUSE AND HUMAN KERATIN-1/KERATIN-10 INTERMEDIATE FILAMENTS AT THE 2-MOLECULE TO 4-MOLECULE LEVEL OF STRUCTURE SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANSMISSION ELECTRON-MICROSCOPY; BOVINE EPIDERMAL KERATIN; COILED-COIL MOLECULES; AMINO-ACID SEQUENCES; ALPHA-KERATIN; INVITRO; CELLS; VIMENTIN; PROTEIN; EXPRESSION AB A number of fundamental questions pertaining to the registration and packing of the constituent coiled-coil molecules in keratin intermediate filaments, and to the regions of the sequences that are responsible for these levels of organization, remain to be elucidated. In this study, small assembly-competent oligomers of mouse and human keratin 1/keratin 10 keratin filaments were cross-linked by the formation of disulfide bonds catalyzed by the copper-phenanthroline reaction. By isolation and characterization of cross-linked peptides, it has been possible to establish two major modes of molecule alignment: an antiparallel arrangement of half-staggered molecules with their 2B segments overlapping and an antiparallel arrangement of molecules in close axial registration. These data confirm earlier models based on theoretical considerations (Crewther, W. G., Dowling, L. M., Steinert, P. M., and Parry, D. A. D. (1983) Int. J. Biol. Macromol. 5, 267-274). Interestingly, these models place the conserved H1 and H2 end domain segments, which flank the ends of the rod domains of the type II keratin 1 chain, in alignment with either the ends of the rod domains and/or with the L2 segment near the center of the rod domains, of the nearest neighbor molecules. Competition experiments with synthetic peptides suggest that the conserved H1 (and possibly H2) subdomain sequences unique to type II keratin chains play pivotal roles in the registration of neighboring molecules in keratin filaments. The data thus afford a molecular explanation for why keratin filaments require a type II chain for assembly in vivo and in vitro. C1 MASSEY UNIV,DEPT PHYS & BIOPHYS,PALMERSTON NORTH,NEW ZEALAND. RP STEINERT, PM (reprint author), NIAMSD,SKIN BIOL BRANCH,BLDG 6,RM 425,BETHESDA,MD 20892, USA. NR 61 TC 89 Z9 89 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2878 EP 2887 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500091 PM 7679103 ER PT J AU HERZOG, CE TSOKOS, M BATES, SE FOJO, AT AF HERZOG, CE TSOKOS, M BATES, SE FOJO, AT TI INCREASED MDR-1/P-GLYCOPROTEIN EXPRESSION AFTER TREATMENT OF HUMAN COLON-CARCINOMA CELLS WITH P-GLYCOPROTEIN ANTAGONISTS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MULTIDRUG-RESISTANCE GENE; DIFFERENTIATING AGENTS; ADENOCARCINOMA CELLS; DRUG-RESISTANCE; MDR1 GENE; LINES; CDNA; VINBLASTINE; MODULATION; VERAPAMIL AB We observed increased levels of mdr-1 mRNA and its protein product P-glycoprotein (Pgp) in the human colon carcinoma cell line, LS 180, and its drug-resistant sublines, LS 180-Ad50 and LS 180-Vb2, after treatment with the Pgp antagonists, verapamil, nifedipine, and cyclosporin A. Increases in mdr-1 RNA were observed within 8 h of the addition of the Pgp antagonists and continued to rise over time. Peak levels were attained after several weeks and were sustained for the duration of treatment up to several months, with a rapid decline in mdr-1 mRNA levels after removal of the Pgp antagonist. Corresponding changes in Pgp were demonstrated with addition and removal of the Pgp antagonists. Increased mdr-1 mRNA was also seen with two other calcium channel blockers, nicardipine and diltiazem, but not with the Pgp antagonists, quinidine and chlorpromazine. Treatment with verapamil or nifedipine was associated with electron microscopic changes consistent with increased differentiation and resulted in increased carcinoembryonic antigen expression, suggesting that the increase in mdr-1 expression was associated with the process of differentiation. Nuclear runoff experiments and inhibition of new RNA synthesis with actinomycin D treatment failed to detect an increase in mdr-1 transcription or stabilization of the mdr-1 mRNA suggesting that the effect of these agents is mediated post-transcriptionally within the nucleus. C1 NCI,PEDIAT BRANCH,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. NCI,MED BRANCH,BETHESDA,MD 20892. NR 32 TC 124 Z9 126 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2946 EP 2952 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500101 PM 8094079 ER PT J AU OSAWA, Y DARBYSHIRE, JF STEINBACH, PJ BROOKS, BR AF OSAWA, Y DARBYSHIRE, JF STEINBACH, PJ BROOKS, BR TI METABOLISM-BASED TRANSFORMATION OF MYOGLOBIN TO AN OXIDASE BY BRCCL3 AND MOLECULAR MODELING OF THE OXIDASE FORM SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LIPID-PEROXIDATION; CYTOCHROME-P-450 HEME; CRYSTAL-STRUCTURE; PROSTHETIC HEME; PROTEIN ADDUCTS; IRON RELEASE; DEGRADATION; HEMOGLOBIN; DYNAMICS; INACTIVATION AB The stoichiometric reductive debromination of BrCCl3 to a trichloromethyl radical by myoglobin caused the prosthetic heme to become covalently cross-linked to the protein moiety and transformed myoglobin from an oxygen storage protein to an oxidase. This was shown in experiments in which oxygen consumption was measured during redox cycling of the altered myoglobin in the presence of ascorbate or an enzymatic reducing system containing diaphorase and NADH. Redox cycling eventually led to loss of the protein-bound heme adduct and oxidase activity of myoglobin. We have used molecular modeling and the known structure of the protein-bound heme adduct to identify probable mechanisms for transformation of myoglobin to an oxidase. Based on these modeling studies, the most likely structure of the experimentally observed adduct involves ligation to the heme iron of the epsilon-nitrogen atom of histidine 97 and/or that of histidine 64. The model structures revealed access of solvent to the heme active site, which could facilitate oxygen reduction. The transformation of myoglobins and perhaps other hemoproteins to oxidases may have toxicological importance in causing the tissue damage resulting from exposure to various xenobiotics and endogenous chemicals as well as explaining how hemoproteins are inactivated during catalysis. C1 NIH,DIV COMP RES & TECHNOL,MOLEC GRAPH & SIMULAT LAB,BETHESDA,MD 20892. RP OSAWA, Y (reprint author), NHLBI,CHEM PATHOL LAB,BLDG 10,RM 8N110,BETHESDA,MD 20892, USA. NR 33 TC 16 Z9 17 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2953 EP 2959 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500102 PM 8428969 ER PT J AU FOROUGH, R ZHAN, X MACPHEE, M FRIEDMAN, S ENGLEKA, KA SAYERS, T WILTROUT, RH MACIAG, T AF FOROUGH, R ZHAN, X MACPHEE, M FRIEDMAN, S ENGLEKA, KA SAYERS, T WILTROUT, RH MACIAG, T TI DIFFERENTIAL TRANSFORMING ABILITIES OF NON-SECRETED AND SECRETED FORMS OF HUMAN FIBROBLAST GROWTH FACTOR-I SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROTEIN-KINASE-C; CELL GROWTH; ENDOTHELIAL-CELLS; GENE; STIMULATION; EXPRESSION; SEQUENCE; SIGNAL; PHOSPHORYLATION; TUMORIGENESIS AB Fibroblast growth factor (FGF)-1(1-154), the precursor for acidic FGF-1(21-154), is a potent angiogenic polypeptide, the structure of which lacks a signal peptide sequence for secretion. To investigate the biological significance of this structural feature, we have attempted forced secretion of FGF-1 through fusion of the entire FGF-1 coding frame with the signal peptide (sp) from the hst/KS3 gene, a secretory member of the heparin-binding growth factor family. We also studied the transforming ability of the signal-less forms of FGF-1 comprising FGF1-154 and FGF-1(21-154). The presence of a soluble and biologically active form of FGF-1 was readily detected in the conditioned medium of NIH 3T3 cells transfected with sp-hst/KS3:FGF-1(1-154) as demonstrated by Western blot analysis and DNA synthesis assays, whereas sp-hst/KS3:FGF-1(21-154) was not detectable in conditioned medium even though the protein was detected in cellular extracts. The secreted form of sp-hst/KS3:FGF-1(1-154) stimulated the proliferation of human umbilical vein endothelial cells in vitro and was able to induce receptor-mediated tyrosine phosphorylation. Furthermore, the forced secretion of biologically active FGF-1 resulted in NIH 3T3 cell transformation as demonstrated by altered morphology in vitro, the formation of discrete colonies in soft agarose, growth under serum-free conditions, and ability to rapidly form highly vascular tumors in vivo. Interestingly, sp-hst/KS3:FGF-1(21-154) also mediated the transition to a transformed phenotype despite the inability to detect extracellular FGF-1 in the media conditioned by these NIH 3T3 cell transfectants. Although the transfection of FGF-1(21-154) yielded similar NIH 3T3 cell morphologic changes, these transfectants did not grow under serum-free conditions or yield colonies in soft agarose, and formed tumors in vivo with delayed kinetics. Furthermore, the FGF-1(1-154) NIH 3T3 cell transfectants did not exhibit morphologic changes, and this may be due to the inability of mRNA to express protein. These data suggest that although non-sp forms of FGF-1 may alter the monolayer phenotype of NIH 3T3 cells in vitro, the ability of FGF-1 to transform NIH 3T3 cells requires the function of a sp-directed secretory pathway and suggests that this pathway increases tumorigenicity in vivo. C1 AMER RED CROSS,JEROME H HOLLAND LAB BIOMED SCI,DEPT MOLEC BIOL,15601 CRABBS BRANCH WAY,ROCKVILLE,MD 20855. NCI,FREDERICK CANC RES & DEV CTR,EXPTL IMMUNOL LAB,EXPTL THERAPEUT SECT,FREDERICK,MD 21702. PRI DYNCORP,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21702. RI Sayers, Thomas/G-4859-2015 FU NCI NIH HHS [N01-CO-74102]; NHLBI NIH HHS [HL32348, HL44336] NR 41 TC 99 Z9 100 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2960 EP 2968 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500103 PM 7679105 ER PT J AU RON, D BOTTARO, DP FINCH, PW MORRIS, D RUBIN, JS AARONSON, SA AF RON, D BOTTARO, DP FINCH, PW MORRIS, D RUBIN, JS AARONSON, SA TI EXPRESSION OF BIOLOGICALLY-ACTIVE RECOMBINANT KERATINOCYTE GROWTH-FACTOR - STRUCTURE-FUNCTION ANALYSIS OF AMINO-TERMINAL TRUNCATION MUTANTS SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SITE-DIRECTED MUTAGENESIS; HEPARIN-BINDING; ENZYMATIC AMPLIFICATION; 3-DIMENSIONAL STRUCTURE; RECEPTOR-BINDING; FACTOR-I; FIBROBLAST; POLYMERASE; PROTEINS; AFFINITY AB Keratinocyte growth factor (KGF) is a newly identified member of the fibroblast growth factor (FGF) family (FGF-7). KGF is expressed by stromal fibroblasts and acts on epithelial cells in a paracrine mode. To facilitate structure/function studies, we utilized the T7 prokaryotic expression system to synthesize this growth factor. Recombinant KGF (rKGF) was mitogenic with a specific activity around 10-fold higher than native KGF. By in vitro mutagenesis, we generated a series of KGF mutants with sequential deletions of the amino-terminal domain, the most divergent region among different FGF members. Mutant proteins, produced in bacteria, were tested for their ability to bind heparin, bind and activate the KGF receptor, and induce DNA synthesis. Heparin binding properties were preserved with deletion of up to 28 amino-terminal residues of the mature KGF but lost by the deletion of an additional 10 residues. Biological activity of mutants with deletions of up to 10 residues was comparable to that of rKGF. However, deletion of 29 residues resulted in significantly reduced ability to stimulate KGF receptor tyrosine-kinase activity and DNA synthesis, although this mutant bound the receptor at high affinity. These characteristics of a partial agonist may be useful in the development of competitive antagonists of KGF action. C1 NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,RM 1E24,BETHESDA,MD 20892. RI Bottaro, Donald/F-8550-2010 OI Bottaro, Donald/0000-0002-5057-5334 NR 31 TC 95 Z9 104 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 1993 VL 268 IS 4 BP 2984 EP 2988 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK815 UT WOS:A1993KK81500106 PM 8428972 ER PT J AU RICE, WG SCHAEFFER, CA HARTEN, B VILLINGER, F SOUTH, TL SUMMERS, MF HENDERSON, LE BESS, JW ARTHUR, LO MCDOUGAL, JS ORLOFF, SL MENDELEYEV, J KUN, E AF RICE, WG SCHAEFFER, CA HARTEN, B VILLINGER, F SOUTH, TL SUMMERS, MF HENDERSON, LE BESS, JW ARTHUR, LO MCDOUGAL, JS ORLOFF, SL MENDELEYEV, J KUN, E TI INHIBITION OF HIV-1 INFECTIVITY BY ZINC-EJECTING AROMATIC C-NITROSO COMPOUNDS SO NATURE LA English DT Article ID MURINE LEUKEMIA-VIRUS; STRANDED NUCLEIC-ACIDS; NUCLEOCAPSID PROTEIN; DIMER FORMATION; FINGER DOMAIN; METAL-IONS; VIRAL-RNA; BINDING; POLYMERASE; SEQUENCE AB RETROVIRAL nucleocapsid and gag-precursor proteins from all known strains of retroviruses contain one or two copies of an invariant sequence, Cys-X2-Cys-X4-His-X4-Cys1,2, that is populated with zinc in mature particles3. Modification of cysteine or histidine residues results in defective packaging of genomic viral RNA and formation of non-infectious particles4-8, making these structures potentially attractive targets for antiviral therapy3,8. We recently reported that aromatic C-nitroso ligands of poly(ADP-ribose) polymerase preferentially destabilize one of the two (CYS-X2-CYS-X28-His-X2-Cys) zinc-fingers with concomitant loss of enzymatic activity9,10, coincidental with selective cytocidal action of the C-nitroso substituted ligands on cancer cells11. Based on the occurrence of (3Cys, 1His) zinc-binding sites in both retroviral nucleocapsid and gag proteins and in poly(ADP-ribose) polymerase12, we reasoned that the C-nitroso compounds may also have antiretroviral effects. We show here that two such compounds, 3-nitrosobenzamide and 6-nitroso-1,2-benzopyrone, inhibit infection of human immunodeficiency virus HIV-1 in human lymphocytes and also eject zinc from isolated HIV-1 nucleocapsid zinc fingers and from intact HIV-1 virions. Thus the design of zinc-ejecting agents that target retroviral zinc fingers represents a new approach to the chemotherapy of AIDS. C1 NCI,FREDERICK CANC RES & DEV CTR,PROGRAM RESOURCES INC DYNCORP,AIDS VACCINE PROGRAM,FREDERICK,MD 21702. EMORY UNIV,SCH MED,DEPT PATHOL & LAB MED,ATLANTA,GA 30322. CTR DIS CONTROL,NCID,DHA,IMMUNOL BRANCH,ATLANTA,GA 30333. OCTAMER INC,TIBURON,CA 94920. SAN FRANCISCO STATE UNIV,ROMBERG TIBURON CTR,ENVIRONM TOXICOL & CHEM LAB,TIBURON,CA 94920. RP RICE, WG (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ANTIVIRAL DRUG MECHANISMS LAB,FREDERICK,MD 21702, USA. RI Bess, Jr., Julian/B-5343-2012 NR 34 TC 187 Z9 189 U1 3 U2 8 PU MACMILLAN MAGAZINES LTD PI LONDON PA PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW SN 0028-0836 J9 NATURE JI Nature PD FEB 4 PY 1993 VL 361 IS 6411 BP 473 EP 475 DI 10.1038/361473a0 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA KK713 UT WOS:A1993KK71300068 PM 8429889 ER PT J AU PANTALEO, G GRAZIOSI, C FAUCI, AS AF PANTALEO, G GRAZIOSI, C FAUCI, AS TI THE IMMUNOPATHOGENESIS OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Review ID MAMMARY-TUMOR VIRUS; T-CELL RECEPTOR; TOXIC LYMPHOCYTES-T; PRIMARY MONONUCLEAR PHAGOCYTES; FOLLICULAR DENDRITIC CELLS; PRIMARY HIV-1 INFECTION; GROWTH-FACTOR-BETA; HTLV-III/LAV; PERIPHERAL-BLOOD; CLONAL DELETION C1 NIAID, IMMUNOREGULAT LAB, BLDG 10, RM 11B13, BETHESDA, MD 20892 USA. RI Pantaleo, Giuseppe/K-6163-2016 NR 106 TC 907 Z9 919 U1 2 U2 30 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD FEB 4 PY 1993 VL 328 IS 5 BP 327 EP 335 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA KK247 UT WOS:A1993KK24700008 PM 8093551 ER PT J AU GIBBS, CJ ASHER, DM BROWN, PW FRADKIN, JE GAJDUSEK, DC AF GIBBS, CJ ASHER, DM BROWN, PW FRADKIN, JE GAJDUSEK, DC TI CREUTZFELDT-JAKOB DISEASE INFECTIVITY OF GROWTH-HORMONE DERIVED FROM HUMAN PITUITARY-GLANDS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter RP GIBBS, CJ (reprint author), NIH,BETHESDA,MD 20892, USA. NR 5 TC 29 Z9 29 U1 1 U2 1 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD FEB 4 PY 1993 VL 328 IS 5 BP 358 EP 359 DI 10.1056/NEJM199302043280520 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA KK247 UT WOS:A1993KK24700027 PM 8419831 ER PT J AU HEALY, B AF HEALY, B TI THE PACE OF HUMAN GENE-TRANSFER RESEARCH QUICKENS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material RP HEALY, B (reprint author), NIH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 3 PY 1993 VL 269 IS 5 BP 567 EP 567 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA KJ444 UT WOS:A1993KJ44400005 PM 8421352 ER PT J AU MILLS, JL HOLMES, LB AARONS, JH SIMPSON, JL BROWN, ZA JOVANOVICPETERSON, LG CONLEY, MR GRAUBARD, BI KNOPP, RH METZGER, BE AF MILLS, JL HOLMES, LB AARONS, JH SIMPSON, JL BROWN, ZA JOVANOVICPETERSON, LG CONLEY, MR GRAUBARD, BI KNOPP, RH METZGER, BE TI MODERATE CAFFEINE USE AND THE RISK OF SPONTANEOUS-ABORTION AND INTRAUTERINE GROWTH-RETARDATION SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID LOW-BIRTH-WEIGHT; PREGNANCY; CONSUMPTION; ASSOCIATION; BEVERAGES; ALCOHOL; COFFEE; LENGTH AB Objective.-To examine the relationship between caffeine consumption during pregnancy and the occurrence of spontaneous abortion and intrauterine growth retardation. Design, Setting, and Patients.-A cohort of 431 women, enrolled in a multicenter study within 21 days of conception, was monitored throughout pregnancy to determine (1) caffeine exposure, (2) exposure to other risk factors, (3) fetal growth as assessed by ultrasonography, and (4) pregnancy outcome. Outcome Measures.-Spontaneous abortion, intrauterine growth, birth weight, and head circumference. Results.-The mean (+/-SD) first-trimester caffeine consumption was not significantly higher in women who aborted (125.9+/-123.1 mg) than in women who delivered liveborn infants (111.6+/-107.0 mg) (P=34). The adjusted odds ratio (OR) for spontaneous abortion was 1.15 (95% confidence interval [CI], 0.89 to 1.49). Early fetal growth, assessed by crown-rump length on ultrasonographic examination, was not affected by caffeine. Although the group consuming the most caffeine (>300 mg/d) had a significantly higher proportion of babies with birth weights and head circumferences below the 10th percentile in the crude analysis, the association with caffeine was no longer significant when other risk factors (notably smoking) were taken into account. The adjusted ORs were 1.11 (95% CI, 0.88 to 1.40) for decreased birth weight and 1.09 (95% CI, 0.86 to 1.37) for smaller head circumference. Conclusions.-Close monitoring of a cohort identified very soon after conception enabled us to identify all abortions after 21 days postconception, monitor intrauterine growth prospectively, and track caffeine use. Despite this intensive surveillance, we found no evidence that moderate caffeine use increased the risk of spontaneous abortion, intrauterine growth retardation, or microcephaly after accounting for other risk factors. C1 BRIGHAM & WOMENS HOSP,DEPT OBSTET & GYNECOL,BOSTON,MA 02115. UNIV PITTSBURGH,MAGEE WOMENS HOSP,DEPT MED,PITTSBURGH,PA 15213. UNIV TENNESSEE CTR HLTH SCI,DEPT OBSTET & GYNECOL,MEMPHIS,TN 38163. UNIV WASHINGTON,SCH MED,DEPT OBSTET & GYNECOL,SEATTLE,WA 98195. UNIV WASHINGTON,SCH MED,DEPT MED,SEATTLE,WA 98195. SANSUM RES FDN,SANTA BARBARA,CA. NCI,BETHESDA,MD 20892. NORTHWESTERN UNIV,SCH MED,DEPT MED,CHICAGO,IL 60611. RP MILLS, JL (reprint author), NICHHD,DIV EPIDEMIOL STAT & PREVENT RES,EPIDEMIOL BRANCH,BETHESDA,MD 20892, USA. NR 31 TC 82 Z9 84 U1 2 U2 11 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 3 PY 1993 VL 269 IS 5 BP 593 EP 597 DI 10.1001/jama.269.5.593 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA KJ444 UT WOS:A1993KJ44400024 PM 8421363 ER PT J AU GAYLIN, DS HELD, PJ PORT, FK HUNSICKER, LG WOLFE, RA KAHAN, BD JONES, CA AGODOA, LYC AF GAYLIN, DS HELD, PJ PORT, FK HUNSICKER, LG WOLFE, RA KAHAN, BD JONES, CA AGODOA, LYC TI THE IMPACT OF COMORBID AND SOCIODEMOGRAPHIC FACTORS ON ACCESS TO RENAL-TRANSPLANTATION SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID DISEASE; AGE AB Objective.-To assess the impact of sociodemographic factors and comorbid conditions on access to renal transplantation for adult US dialysis patients with end-stage renal disease (ESRD). Design.-Cohort analytic study. Data on comorbid conditions at onset of ESRD were abstracted from patients' medical records and matched to sociodemographic and ESRD data from the United States Renal Data System database. Setting.-United States Medicare dialysis population. Patients.-Random, national sample of ESRD patients starting dialysis in 1986 and 1987 (n=4118). Main Outcome Measure.-Time to first renal transplant (living or cadaver donor) since onset of ESRD regressed with two nested Cox proportional hazards models, first against sociodemographic factors alone, and then against sociodemographic factors and comorbid conditions. Results.-Cardiovascular diseases are most predictive of who receives a transplant; patients with coronary heart disease, congestive heart failure, or left ventricular hypertrophy showed lower transplantation rates relative to patients without the disease (relative rate [RR]=0.65 to 0.80, P<.05 each). Obese patients and patients with peripheral vascular disease also showed lower transplantation rates (RR=0.65 to 0.75, P<.05 each). Previously reported sociodemographic effects of lower transplantation rates for older patients, women, nonwhite patients, and lower income patients were confirmed (P<.01). Sociodemographic effects remained essentially unchanged when adjusted for comorbid conditions. Conclusions.-These findings indicate that sociodemographics have strong independent effects on access to transplantation that cannot be explained away as ''surrogate'' effects related to comorbid factors. Furthermore, the results suggest that lower mortality rates for transplant recipients relative to dialysis patients are due, in part, to a healthier case mix among patients receiving transplants. C1 UNIV MICHIGAN,SCH PUBL HLTH,DEPT EPIDEMIOL,ANN ARBOR,MI 48109. UNIV MICHIGAN,SCH PUBL HLTH,DEPT BIOSTAT,ANN ARBOR,MI 48109. UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI 48104. UNIV IOWA,DEPT INTERNAL MED,IOWA CITY,IA 52242. UNIV TEXAS,SCH MED,DIV IMMUNOL & ORGAN TRANSPLANTAT,HOUSTON,TX 77025. NIDDKD,DIV KIDNEY UROL & HEMATOL DIS,BETHESDA,MD. RP GAYLIN, DS (reprint author), URBAN INST,2100 M ST NW,SUITE 400 USRDS,WASHINGTON,DC 20037, USA. FU NIDDK NIH HHS [N0 1-DK-8-2234] NR 14 TC 213 Z9 213 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 3 PY 1993 VL 269 IS 5 BP 603 EP 608 DI 10.1001/jama.269.5.603 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA KJ444 UT WOS:A1993KJ44400026 PM 8421364 ER PT J AU KAPIKIAN, AZ AF KAPIKIAN, AZ TI VIRAL GASTROENTERITIS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID INFECTIOUS NONBACTERIAL GASTROENTERITIS; NON-BACTERIAL GASTROENTERITIS; NORWALK VIRUS; ACUTE DIARRHEA; UNITED-STATES; OUTBREAKS; ROTAVIRUS; CHILDREN; THERAPY; AGENT RP KAPIKIAN, AZ (reprint author), NIAID,EPIDEMIOL SECT,INFECT DIS LAB,BLDG 7,ROOM 103,BETHESDA,MD 20892, USA. NR 35 TC 51 Z9 56 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD FEB 3 PY 1993 VL 269 IS 5 BP 627 EP 630 DI 10.1001/jama.269.5.627 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA KJ444 UT WOS:A1993KJ44400031 PM 8380619 ER PT J AU TARABOLETTI, G BELOTTI, D GIAVAZZI, R SOBEL, ME CASTRONOVO, V AF TARABOLETTI, G BELOTTI, D GIAVAZZI, R SOBEL, ME CASTRONOVO, V TI ENHANCEMENT OF METASTATIC POTENTIAL OF MURINE AND HUMAN-MELANOMA CELLS BY LAMININ RECEPTOR PEPTIDE-G - ATTACHMENT OF CANCER-CELLS TO SUBENDOTHELIAL MATRIX AS A PATHWAY FOR HEMATOGENOUS METASTASIS SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID TUMOR-CELLS; EXTRACELLULAR-MATRIX; ADHESION; FIBRONECTIN; COLLAGENASE; EXPRESSION; MOLECULES; INVASION; FRAGMENT; DOMAINS AB Background: Stable anchorage of circulating cancer cells to the vasculature is a critical step in the formation of hematogenous metastases. Although the basement membrane glycoprotein laminin clearly plays a crucial role in this event, the exact interactive pathways among cancer cells, laminin, and the vessel wall have not been elucidated. In a previous study, we identified synthetic peptide G, which contains the laminin-binding domain of the 67-kd laminin receptor and which inhibits tumor cell adhesion to endothelial cells. Purpose: To assess the role of the interaction between laminin and the 67-kd laminin.receptor in hematogenous metastasis formation, we studied the effect of peptide G on melanoma cell behavior in vivo and in vitro. Methods: The effect of peptide G and control peptides was studied in vivo on lung retention and colonizing potential of murine (B16BL6) and human (A2058) melanoma cells injected intravenously in C57BL/6 and nude mice, respectively. In addition, their effect on cell adhesion and chemotaxis to laminin and on binding of iodine 125-labeled laminin to cells was studied in vitro. Results: In vivo, pretreatment of cells with peptide G resulted in a two- to 10-fold significant increase in the number of experimental lung metastases. A significant relative increase in lung retention of peptide G-treated tumor cells was observed 48 hours after injection, although after 4 hours a partial reduction was observed. In vitro, peptide G significantly increased laminin binding and cancer cell adhesion to laminin and subendothelial matrix, whereas chemotaxis to laminin was significantly inhibited. Conclusions: Peptide G differentially affected the biological response of cancer cells to laminin. In vitro, it increased laminin binding and cell adhesion to laminin and subendothelial matrix, whereas it inhibited cell chemotaxis to laminin. In vivo, the overall effect of peptide G was an augmentation of lung metastasis. Implications: Our findings suggest that direct adhesion of tumor cells to the subendothelial matrix is a main pathway for hematogenous metastases and that tumor cell-matrix interaction may be more relevant than tumor cell-endothelial cell attachment in this process. C1 IST RIC FARMACOL MARIO NEGRI,BERGAMO,ITALY. NCI,DIV CANC BIOL DIAGN & CTR,PATHOL LAB,BETHESDA,MD 20892. RP TARABOLETTI, G (reprint author), IST MARIO NEGRI,VIA GAVAZZENI 11,I-2400 BERGAMO,ITALY. NR 35 TC 47 Z9 47 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD FEB 3 PY 1993 VL 85 IS 3 BP 235 EP 240 DI 10.1093/jnci/85.3.235 PG 6 WC Oncology SC Oncology GA KJ687 UT WOS:A1993KJ68700017 PM 8423629 ER PT J AU ARCHER, SJ BAX, A ROBERTS, AB SPORN, MB OGAWA, Y PIEZ, KA WEATHERBEE, JA TSANG, MLS LUCAS, R ZHENG, BL WENKER, J TORCHIA, DA AF ARCHER, SJ BAX, A ROBERTS, AB SPORN, MB OGAWA, Y PIEZ, KA WEATHERBEE, JA TSANG, MLS LUCAS, R ZHENG, BL WENKER, J TORCHIA, DA TI TRANSFORMING GROWTH FACTOR-BETA-1 - NMR SIGNAL ASSIGNMENTS OF THE RECOMBINANT PROTEIN EXPRESSED AND ISOTOPICALLY ENRICHED USING CHINESE-HAMSTER OVARY CELLS SO BIOCHEMISTRY LA English DT Article ID MAGNETIC-RESONANCE SPECTROSCOPY; 3-DIMENSIONAL HETERONUCLEAR NMR; LARGER PROTEINS; FACTOR-BETA; COHERENCE TRANSFER; H-1-NMR SPECTRA; C-13; N-15; SEQUENCE; INTERLEUKIN-1-BETA AB The transforming growth factor betas are a homologous family of multifunctional cytokines that regulate cell growth and differentiation. As a prelude to studies of the solution structure and dynamics of TGF-beta1, we report virtually complete assignment of H-1 and N-15 resonances for this 25-kDa homodimeric protein. Recombinant TGF-beta1 was expressed in Chinese hamster ovary cells. The cells were grown either in a completely N-15-enriched medium or in a medium containing selectively C-13,N-15-labeled amino acids to obtain either uniformly or specifically labeled protein, respectively. Two- and three-dimensional heteronuclear edited magnetic resonance spectra of the uniformly N-15-labeled protein and three samples selectively labeled with C-13 and N-15 yielded assignments for 96% of the backbone amide and Calpha protons and 87% of the side chain protons. To our knowledge, this is the first report of the use of an animal cell expression system to obtain extensive isotopic enrichment in order to sequentially assign a protein. The methodology described herein for the isotopic enrichment and resonance assignments of TGF-beta1 should be generally applicable to other eukaryotic proteins expressed by animal cells. C1 NIDR, BONE RES BRANCH, BETHESDA, MD 20892 USA. NIDDKD, CHEM PHYS LAB, BETHESDA, MD 20892 USA. NCI, CHEMOPREVENT LAB, BETHESDA, MD 20892 USA. CELTRIX PHARMACEUT INC, SANTA CLARA, CA 95054 USA. THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA. NIH, FOGARTY INT CTR, BETHESDA, MD 20892 USA. R&D SYST INC, MINNEAPOLIS, MN 55413 USA. FU NIGMS NIH HHS [GM13620] NR 43 TC 44 Z9 45 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 2 PY 1993 VL 32 IS 4 BP 1152 EP 1163 DI 10.1021/bi00055a021 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK545 UT WOS:A1993KK54500021 PM 8424942 ER PT J AU ARCHER, SJ BAX, A ROBERTS, AB SPORN, MB OGAWA, Y PIEZ, KA WEATHERBEE, JA TSANG, MLS LUCAS, R ZHENG, BL WENKER, J TORCHIA, DA AF ARCHER, SJ BAX, A ROBERTS, AB SPORN, MB OGAWA, Y PIEZ, KA WEATHERBEE, JA TSANG, MLS LUCAS, R ZHENG, BL WENKER, J TORCHIA, DA TI TRANSFORMING GROWTH FACTOR-BETA-1 - SECONDARY STRUCTURE AS DETERMINED BY HETERONUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY SO BIOCHEMISTRY LA English DT Article ID MULTIPLE QUANTUM COHERENCE; NMR-SPECTROSCOPY; STAPHYLOCOCCAL NUCLEASE; 3-DIMENSIONAL STRUCTURES; PROTEIN-STRUCTURE; PEPTIDE-BOND; TGF-BETA; SPECTRA; 2D; INTERLEUKIN-1-BETA AB Virtually complete backbone NMR signal assignments have been reported for transforming growth factor beta1 (TGF-beta1) [Archer et al. (1993) Biochemistry (preceding paper in this issue)]. Herein we report the secondary structure of the protein in solution on the basis of these assignments and proton NOE's observed in a variety of 2D and 3D heteronuclear NMR spectra. Regular elements of secondary structure derived from the NOE data consist of (a) three helices spanning residues Y58-H68, F24-G29, and N5-F8 and (b) several pairs of two-stranded antiparallel beta-sheets. The longest two-stranded sheet runs from residue L83 to V106 with a type II reverse turn at G93-R94 and a chain twist at residue N103-M104. These elements of regular structure were confirmed by hydrogen exchange, chemical shift, and coupling constant data. With the exception of residues G46-S53, which exhibit relatively few and weak intraresidue NOE's, residues in the rest of the protein adopt an irregular but well-defined structure. All peptide bonds are trans except for a cis peptide bond between Glu35 and Pro36. The structural characteristics observed for TGF-beta1 in solution generally agree closely with the recently derived crystal structures of TGF-beta2 [Daopin et al. (1992) Science 257, 369-374; Schlunegger & Grutter (1992) Nature 358, 430-434]. Several noteworthy differences were observed that may be related to function. C1 NIDR, BONE RES BRANCH, BETHESDA, MD 20892 USA. NIDDKD, CHEM PHYS LAB, BETHESDA, MD 20892 USA. NCI, CHEMOPREVENT LAB, BETHESDA, MD 20892 USA. CELTRIX PHARMACEUT INC, SANTA CLARA, CA 95054 USA. THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA. R&D SYST INC, MINNEAPOLIS, MN 55413 USA. FU NIGMS NIH HHS [GM13620] NR 36 TC 59 Z9 60 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 2 PY 1993 VL 32 IS 4 BP 1164 EP 1171 DI 10.1021/bi00055a022 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KK545 UT WOS:A1993KK54500022 PM 8424943 ER PT J AU TALAN, MI ENGEL, BT AF TALAN, MI ENGEL, BT TI MORNING INCREASE IN WHOLE-BLOOD VISCOSITY - A CONSEQUENCE OF A HOMEOSTATIC NOCTURNAL HEMODYNAMIC PATTERN SO ACTA PHYSIOLOGICA SCANDINAVICA LA English DT Article DE DIURNAL; HEMODYNAMICS; PLASMA VOLUME ID SUDDEN CARDIAC DEATH; MYOCARDIAL-INFARCTION; CIRCADIAN VARIATION; CONTRIBUTING FACTOR; DIURNAL-VARIATION; PLASMA VISCOSITY; HEMATOCRIT; FREQUENCY; PRESSURE; RHEOLOGY AB In a series of studies, we have shown that in non-human primates there is a consistent overnight fall in cardiac output and central venous pressure, and a rise in total peripheral resistance. This haemodynamic pattern is associated with a higher haematocrit level in the morning suggesting that these changes in the circulation are homeostatic adjustments to a nighttime fall in plasma volume. The present study was designed to test the hypothesis that in the morning whole blood viscosity also is higher. Whole blood viscosity was measured at shear rates of 450, 225, 90, 45, and 22.5 s-1 in each of six monkeys, on four occasions, at 2-week intervals, at 17.00 and 09.00 h the next morning. The average haematocrit was 4.2% higher in the morning than in the previous evening (P < 0.01). Viscosity decreased monotonically at progressively higher shear rates but was always significantly higher in the morning than in the evening (P < 0.01 at all shear rates). When viscosity was adjusted by covarying for haematocrit level, the morning/evening differences became non-significant. However, the morning/evening differences in linear trend of shear stress as a function of shear rate persisted. These findings add further support to our hypothesis that the nocturnal haemodynamic pattern in non-human primates is related to a reduction in plasma volume, and they also suggest that the morning rise in haematocrit is a major contributing factor to the elevated viscosity. RP TALAN, MI (reprint author), NIA,BEHAV SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 36 TC 9 Z9 9 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0001-6772 J9 ACTA PHYSIOL SCAND JI Acta Physiol. Scand. PD FEB PY 1993 VL 147 IS 2 BP 179 EP 183 DI 10.1111/j.1748-1716.1993.tb09487.x PG 5 WC Physiology SC Physiology GA KP082 UT WOS:A1993KP08200005 PM 8475744 ER PT J AU GILL, DS KETTER, TA POST, RM AF GILL, DS KETTER, TA POST, RM TI ANTIDEPRESSANT RESPONSE TO SLEEP-DEPRIVATION AS A FUNCTION OF TIME INTO DEPRESSIVE EPISODE IN RAPIDLY CYCLING BIPOLAR PATIENTS SO ACTA PSYCHIATRICA SCANDINAVICA LA English DT Article DE SLEEP DEPRIVATION; DEPRESSION; BIPOLAR DISORDER ID ENDOGENOUS-DEPRESSION; AFFECTIVE-ILLNESS; THERAPY; LITHIUM; MOOD AB Three patients with treatment-resistant rapidly cycling bipolar disorder were studied with multiple sleep deprivations (SD) during several depressive episodes to assess the effect of phase or duration of a depressive episode on SD response. There was little response to SD early in a depressive episode, but responses were often robust late in an episode, sometimes triggering its termination. In 2 subjects, the duration of antidepressant response to SD increased linearly as time into episode increased. Neither the number of SD given in an episode nor the medication status of the patients appeared to account for the observed increases in antidepressant response. These results suggest that the neurobiological substrates underlying depression may change over the course of an episode, resulting in an increased responsivity to sleep deprivation later compared with earlier in the course of an episode in rapidly cycling patients. The generalizability of these findings to unipolar patients remains to be explored. C1 NIMH,BIOL PSYCHIAT BRANCH,9000 ROCKVILLE PIKE,BLDG 100,ROOM 3N212,BETHESDA,MD 20892. NR 31 TC 10 Z9 10 U1 1 U2 1 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0001-690X J9 ACTA PSYCHIAT SCAND JI Acta Psychiatr. Scand. PD FEB PY 1993 VL 87 IS 2 BP 102 EP 109 DI 10.1111/j.1600-0447.1993.tb03338.x PG 8 WC Psychiatry SC Psychiatry GA KN635 UT WOS:A1993KN63500005 PM 8447235 ER PT J AU HARFORD, TC AF HARFORD, TC TI STABILITY AND PREVALENCE OF DRINKING AMONG YOUNG-ADULTS SO ADDICTION LA English DT Article AB This data note draws upon the National Longitudinal Survey (NLS) of Labor Market Experience in Youth beginning at ages 17-24 to describe the stability and prevalence of alcohol use over a 6-year period up to ages 23-30. Approximately 70% of men and 58% of women maintained their drinking status throughout the study. The onset of current and heavier drinking decreased with increasing age while the offset of current and heavier drinking increased with increasing age. The absence of current drinking was unrelated to age for both men and women as was the presence of heavier drinking among men. RP HARFORD, TC (reprint author), NIAAA,DIV BIOMETRY & EPIDEMIOL,5600 FISHERS LANE,ROOM 14C-26,ROCKVILLE,MD 20857, USA. NR 5 TC 17 Z9 17 U1 0 U2 0 PU CARFAX PUBL CO PI ABINGDON PA PO BOX 25, ABINGDON, OXFORDSHIRE, ENGLAND OX14 3UE SN 0965-2140 J9 ADDICTION JI Addiction PD FEB PY 1993 VL 88 IS 2 BP 273 EP 277 DI 10.1111/j.1360-0443.1993.tb00811.x PG 5 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA KM841 UT WOS:A1993KM84100016 PM 8220065 ER PT J AU MUKHOPADHYAYA, R SADAIE, MR AF MUKHOPADHYAYA, R SADAIE, MR TI NUCLEOTIDE-SEQUENCE ANALYSIS OF HTLV-I ISOLATED FROM CEREBROSPINAL-FLUID OF A PATIENT WITH TSP HAM - COMPARISON TO OTHER HTLV-I ISOLATES SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID T-CELL LEUKEMIA; VIRUS TYPE-I; TROPICAL SPASTIC PARAPARESIS; LONG TERMINAL REPEAT; AFRICAN PATIENTS; ENVELOPE GENE; MYELOPATHY; RETROVIRUS; IDENTIFICATION; VARIANTS AB Human T-cell leukemia virus type I (HTLV-I) has been associated with adult T-cell leukemia/lymphona and the chronic neurologic disorder tropical spastic paraparesis/HTLV-I associated myelopathy (TSP/HAM). To study the genetic structure of the virus associated with TSP/HAM, we have obtained and sequenced a partial genomic clone from HTLV-I-positive cell line established from cerebrospinal fluid (CSF) of a Jamaican patient with TSP/HAM. This clone consisted of a 4.3-kb viral sequence containing the 5' long terminal repeat (LTR), gag, and N-terminal portion of the pol gene, with an overall 1.3% sequence variation resulting from mostly nucleotide substitutions, as compared to the prototype HTLV-I ATK-1. The gag and pol regions showed only 1.4% and 1.2% nucleotide variations, respectively. However, the U3 region of the LTR showed the highest sequence variation (3.6%), where several changes appear to be common among certain TSP/HAM isolates. Several of these changes reside within the 21-bp boundaries and the Tax-responsive element. It would be important to determine if the observed changes are sufficient to cause neurologic disorders similar to the murine leukemia virus system or simply reflect the divergent pool of HTLV-I form different geographic locations. At this time, we cannot rule out the possibility that the observed changes have either direct or indirect significance for the HTLV-I pathogenesis in TSP/HAM. C1 NCI,TUMOR CELL BIOL LAB,BLDG 37,RM 6A11,BETHESDA,MD 20892. NR 24 TC 14 Z9 14 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1993 VL 9 IS 2 BP 109 EP 114 DI 10.1089/aid.1993.9.109 PG 6 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA KQ327 UT WOS:A1993KQ32700002 PM 8457377 ER PT J AU LEVINE, PH JACOBSON, S ELLIOTT, R CAVALLERO, A COLCLOUGH, G DORRY, C STEPHENSON, C KNIGGE, RM DRUMMOND, J NISHIMURA, M TAYLOR, ME WIKTOR, S SHAW, GM AF LEVINE, PH JACOBSON, S ELLIOTT, R CAVALLERO, A COLCLOUGH, G DORRY, C STEPHENSON, C KNIGGE, RM DRUMMOND, J NISHIMURA, M TAYLOR, ME WIKTOR, S SHAW, GM TI HTLV-II INFECTION IN FLORIDA INDIANS SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID CELL LYMPHOTROPIC VIRUS; GUAYMI INDIANS; LEUKEMIA LYMPHOMA; PANAMA; POPULATION AB A significantly increased prevalence of antibodies to human T-cell leukemia virus (HTLV) has been described in several native American populations in the United States and Latin America. Initial virologic studies indicate that HTLV-II is the predominant virus responsible for this antibody pattern. We obtained blood samples from 106 Seminole Indians living on four reservations in Southern Florida. Seropositivity to HTLV-I/II was found in 14 (13.2%) of these individuals. Polymerase chain reaction (PCR) documented HTLV-II and the absence of HTLV-I in 7 of the 9 donors available for follow-up testing of white blood cells. Evaluation of various risk factors excluded blood transfusion or intravenous drug use as an important contributing factor to the HTLV-II seroprevalence rate. These studies support the hypothesis that HTLV-II is endemic in many native American tribes in the Western hemisphere. C1 NINCDS,BETHESDA,MD 20892. SEMINOLE INDIAN RESERVAT,HOLLYWOOD,FL 33204. RES TRIANGLE INST,ROCKVILLE,MD 20852. UNIV ALABAMA,BIRMINGHAM,AL 35294. PROGRAM RESOURCES INC DYNCORP,FREDERICK,MD 21702. RP LEVINE, PH (reprint author), NCI,VIRAL EPIDEMIOL BRANCH,EPN 434,BETHESDA,MD 20892, USA. FU NCI NIH HHS [N01-CP-95612] NR 18 TC 75 Z9 78 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1993 VL 9 IS 2 BP 123 EP 127 DI 10.1089/aid.1993.9.123 PG 5 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA KQ327 UT WOS:A1993KQ32700004 PM 8457379 ER PT J AU PATEL, M YANAGISHITA, M RODERIQUEZ, G BOUHABIB, DC ORAVECZ, T HASCALL, VC NORCROSS, MA AF PATEL, M YANAGISHITA, M RODERIQUEZ, G BOUHABIB, DC ORAVECZ, T HASCALL, VC NORCROSS, MA TI CELL-SURFACE HEPARAN-SULFATE PROTEOGLYCAN MEDIATES HIV-1 INFECTION OF T-CELL LINES SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; FIBROBLAST GROWTH-FACTOR; HIGH-AFFINITY RECEPTOR; DEXTRAN SULFATE; SELECTIVE INHIBITORS; VIRION BINDING; POLYSACCHARIDES; INVITRO; POTENT; REPLICATION AB The role of cell-surface proteoglycans in human immunodeficiency virus (HIV) infection of T-cell lines was investigated. HIV-1-susceptible lymphoblastic T-cell lines, MT-4 and H9, were analyzed for proteoglycan synthesis and found to make heparan sulfate (HS) and chondroitin sulfate proteoglycans. Enzymatic treatment of these cells with heparitinase, but not chondroitinase, significantly prevented HIV-1(IIIB) infection as measured by inhibition of cytopathicity, reverse transcriptase production, and syncytia formation. Sulfation of glycosaminoglycans HS chains was critical to viral entry as shown by inhibition of viral infection with sodium chlorate and its specific reversal with exogenous sulfate addition. Quantitation of direct virus binding to cells showed that treatment of cells with heparitinase inhibited HIV-1 binding to the T-cell surface. Exogenous HS added to cultures inhibited virus infection in a manner analogous to dextran sulfate, further supporting a functional role for HS in HIV-1 binding. These results provide evidence for participation of cell-surface HS proteoglycans in HIV-cell attachment and virus entry. C1 US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,8800 ROCKVILLE PIKE,NIH BLDG 29A,ROOM 2D-20,BETHESDA,MD 20892. NIDR,BONE RES BRANCH,BETHESDA,MD 20892. NR 23 TC 209 Z9 212 U1 0 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD FEB PY 1993 VL 9 IS 2 BP 167 EP 174 DI 10.1089/aid.1993.9.167 PG 8 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA KQ327 UT WOS:A1993KQ32700011 PM 8096145 ER PT J AU FRENCH, TA MASSERANO, JM WEINER, N AF FRENCH, TA MASSERANO, JM WEINER, N TI INFLUENCE OF THYROTROPIN-RELEASING-HORMONE AND CATECHOLAMINERGIC INTERACTIONS ON CNS ETHANOL SENSITIVITY SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE LONG-SLEEP AND SHORT-SLEEP MICE; THYROTROPIN-RELEASING HORMONE; TYROSINE HYDROXYLASE ID CENTRAL-NERVOUS-SYSTEM; SS MICE; MEDIATING DIFFERENCES; LONG-SLEEP; TRH; BRAIN; RAT; RECEPTORS; LS; MECHANISMS AB The role of catecholamine neuronal systems in mediating the analeptic and thermogenic effects of thyrotropin-releasing hormone (TRH) was examined in long-sleep (LS) and short-sleep (SS) mice. TRH 10.1 to 40 mug, intracerebroventricularly (icv)] was associated with a reduction in the sleep times of LS mice, but no dose of TRH had any effect on sleep times of SS mice. However, TRH (20 mug, icv) produced a 1.00 to 1.5-degrees-C attenuation of the ethanol-induced hypothermia in both LS and SS mice. TRH did not change the rate of ethanol elimination in either line of mice, suggesting that the reduction in LS sleep times and attenuation of LS and SS hypothermia were due to decreased CNS ethanol sensitivity rather than an increase in the rate of ethanol metabolism. TRH (20 mug, icv) given alone produced an activation of central and peripheral catecholamine systems in LS, but not SS mice, as reflected by an increase in the in vivo tyrosine hydroxylase (TH) activity in the brain and adrenal gland. TRH, given with ethanol, prevented or attenuated ethanol-induced decreases in the brain and adrenal gland in vivo TH activity in LS mice but not SS mice. Thus, there was an association between the ability of TRH to produce an activation of catecholamine neuronal systems (increased rate of catecholamine biosynthesis) and the analeptic action of TRH to reduce the CNS depressant effects of ethanol decreased sleep times). TRH was able to attenuate the hypothermic effect of ethanol in both LS and SS mice, despite a lack of effect on TH activity, suggesting that catecholamine neurons may not have a major role in the thermogenic action of TRH in LS and SS mice. C1 ST ELIZABETH HOSP,NATL INST MENTAL HLTH,CTR NEUROSCI,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. RP FRENCH, TA (reprint author), UNIV COLORADO,HLTH SCI CTR,ALCOHOL RES CTR,DEPT PHARMACOL C236,4200 E 9TH AVE,DENVER,CO 80262, USA. FU PHS HHS [03527] NR 36 TC 17 Z9 17 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD FEB PY 1993 VL 17 IS 1 BP 99 EP 106 DI 10.1111/j.1530-0277.1993.tb00732.x PG 8 WC Substance Abuse SC Substance Abuse GA KP433 UT WOS:A1993KP43300015 PM 8095774 ER PT J AU ZHANG, J JONES, M SHANDAS, R VALDESCRUZ, LM MURILLO, A YAMADA, I KANG, SU WEINTRAUB, RG SHIOTA, T SAHN, DJ AF ZHANG, J JONES, M SHANDAS, R VALDESCRUZ, LM MURILLO, A YAMADA, I KANG, SU WEINTRAUB, RG SHIOTA, T SAHN, DJ TI ACCURACY OF FLOW CONVERGENCE-ESTIMATES OF MITRAL REGURGITANT FLOW-RATES OBTAINED BY USE OF MULTIPLE COLOR FLOW DOPPLER M-MODE ALIASING BOUNDARIES - AN EXPERIMENTAL ANIMAL STUDY SO AMERICAN HEART JOURNAL LA English DT Article ID ORIFICE; INVITRO; JETS; QUANTIFICATION; LIMITATIONS; VOLUME; SIZE AB The proximal flow Convergence method of multiplying color Doppler aliasing velocity by flow convergence surface area has yielded a new means of quantifying flow rate by noninvasively derived measurements. Unlike previous methods of visualizing the turbulent jet of mitral regurgitation on color flow Doppler mapping, flow convergence methods are less influenced by machine factors because of the systematic structure of the laminar flow convergence region. However, recent studies have demonstrated that the flow rate calculated from the first aliasing boundary of color flow Doppler imaging is dependent on orifice size, flow rate, aliasing velocity and therefore on the distance from the orifice chosen for measurement. In this study we calculated the regurgitant flow rates acquired by use of multiple proximal aliasing boundaries on color Doppler M-mode traces and assessed the effect of distances of measurement and aliasing velocities on the calculated regurgitant flow rate. Six sheep with surgically induced mitral regurgitation were studied. The distances from the mitral valve leaflet M-mode line to the first, second, and third sequential aliasing boundaries on color Doppler M-mode traces were measured and converted to the regurgitant flow rates calculated by applying the hemispheric flow equation and averaging instantaneous flow rates throughout systole. The flow rates that were calculated from the first, second, and third aliasing boundaries correlated well with the actual regurgitant flow rates (r = 0.91 to 0.96). The mean percentage error from the actual flow rates were 151% for the first aliasing boundary, 7% for the second aliasing boundary, and -43% for the third aliasing boundary; and the association between aliasing velocities and calculated flow rates indicates an inverse relationship, which suggests that in this model, there were limited velocity-distance combinations that fit with a hemispheric assumption for flow convergence geometry. The second aliasing boundary with an aliasing velocity, of 102 cm/sec, (which was achieved by use of a 4 kHz pulse repetition frequency, a 3.75 MHz transducer, and no color baseline shift), provided the closest fit to the actual regurgitant flow rates (r = 0.99; y = 0.95x + 0.07). The averaged calculated flow rates from all aliasing velocities also resulted in excellent correlation (r = 0.97; y = 0.99x + 0.5). A hemispheric flow convergence method that is based on color Doppler M-mode echocardiography is a feasible and automatable method for quantifying mitral regurgitant rate. The inversely changing relationship between the calculated regurgitant flow rates and the aliasing velocities in our study suggests that the shape of aliasing boundary may change from ''elliptic paraboloid'' to a more ''plate-like'' flattened hemielliptic configuration when flow approaches the orifice. With knowledge of changing flow geometry factored into this method and the ability to perform this calculation at multiple velocity-distance combinations to average this data, the accuracy of this method should increase. C1 UNIV CALIF SAN DIEGO,MED CTR,DIV PEDIAT CARDIOL,LA JOLLA,CA 92093. NHLBI,BETHESDA,MD 20892. OI Shandas, Robin/0000-0002-9473-7542 FU NHLBI NIH HHS [R01 HL43287] NR 42 TC 30 Z9 31 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-8703 J9 AM HEART J JI Am. Heart J. PD FEB PY 1993 VL 125 IS 2 BP 449 EP 458 DI 10.1016/0002-8703(93)90025-5 PN 1 PG 10 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA KL170 UT WOS:A1993KL17000025 PM 8427140 ER PT J AU ALGER, S LARSON, K BOYCE, VL SEAGLE, H FONTVIEILLE, AM FERRARO, RT RISING, R RAVUSSIN, E AF ALGER, S LARSON, K BOYCE, VL SEAGLE, H FONTVIEILLE, AM FERRARO, RT RISING, R RAVUSSIN, E TI EFFECT OF PHENYLPROPANOLAMINE ON ENERGY-EXPENDITURE AND WEIGHT-LOSS IN OVERWEIGHT WOMEN SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE INDIRECT CALORIMETRY; BODY COMPOSITION; 24-H ENERGY EXPENDITURE; SYMPATHOMIMETIC ID ADIPOSE-TISSUE THERMOGENESIS; CHRONIC EPHEDRINE TREATMENT; BLOOD-PRESSURE; PLACEBO; DETERMINANTS; COMBINATION; ADULTS; RAT AB The effect of phenylpropanolamine (PPA), a noncatecholamine sympathomimetic weight-loss agent, on energy expenditure (EE) and substrate oxidation was measured in a respiratory chamber in 24 overweight women after 4 d of treatment (PPA or placebo) during weight maintenance and after 7 wk of treatment on a hypoenergetic diet (70% of measured baseline 24-h EE). Twelve women (37 +/- 2 y, 74 +/- 6 kg, 33 +/- 1% body fat) were randomly assigned to the PPA group [75 mg osmotic release oral system (OROS)-PPA/d] and 12 (xBAR +/- SEM: 38 +/- 2 y, 79 +/- 1 kg, 37 +/- 1% body fat) to the placebo group. Baseline measurements of 24-h EE (7849 +/- 226 vs 7834 +/- 142 kJ/d), basal metabolic rate (BMR) and 24-h respiratory quotient (RQ) were comparable between PPA and placebo groups. After 4 d of treatment, there was no significant effect of PPA on 24-h EE, BMR, and 24-h RQ compared with placebo. Over the 7-wk diet period, however, the PPA group (n = 8) had greater weight loss than the placebo group (n = 10): -5.0 +/- 0.5 vs -3.0 +/- 0.4 kg (P < 0.05). The changes in 24-h EE and 24-h RQ over the 7 wk were not different between the groups. We conclude that weight loss is enhanced by OROS-PPA, but this change was not explained by changes in 24-h EE or 24-h RQ. The small number of subjects may have hindered detection of subtle differences in energy metabolism. C1 NIDDKD,CLIN DIABET & NUTR SECT,4212 N 16TH ST,ROOM 541,PHOENIX,AZ 85016. ALBANY MED CTR,DIV CLIN NUTR,ALBANY,NY 12208. NR 41 TC 14 Z9 16 U1 0 U2 0 PU AMER SOC CLINICAL NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 1993 VL 57 IS 2 BP 120 EP 126 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA KK283 UT WOS:A1993KK28300002 PM 8424378 ER PT J AU JOHNSTON, PG KLEINER, D COWAN, KH AF JOHNSTON, PG KLEINER, D COWAN, KH TI FAZARABINE - A REPORT OF RESPONSE IN A PATIENT WITH MULTIPLY RELAPSED EMBRYONAL CELL-CARCINOMA SO AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS LA English DT Article DE ALPHA-FETOPROTEIN; TUMOR MARKER; PARTIAL REMISSION; ARA-AC ID DNA; METHYLATION AB This report describes a patient with multiply relapsed and advanced metastatic embryonal cell carcinoma who achieved a pathological partial remission (PR) following treatment with a Phase I chemotherapeutic agent, arabinosyl-5-azacytosine (Ara-AC), followed by orchiectomy. The antimetabolic Ara-AC is worthy of further study in patients with germ-cell tumors. It would appear that the antimetabolite Ara-AC may have significant activity in patients with germ-cell tumors. C1 NCI,MED BRANCH,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. OI Kleiner, David/0000-0003-3442-4453 NR 9 TC 2 Z9 2 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0277-3732 J9 AM J CLIN ONCOL-CANC JI Am. J. Clin. Oncol.-Cancer Clin. Trials PD FEB PY 1993 VL 16 IS 1 BP 34 EP 37 DI 10.1097/00000421-199302000-00009 PG 4 WC Oncology SC Oncology GA KK744 UT WOS:A1993KK74400009 PM 7678714 ER PT J AU HAYES, RB POTTERN, LM GREENBERG, R SCHOENBERG, J SWANSON, GM LIFF, J SCHWARTZ, AG BROWN, LM HOOVER, RN AF HAYES, RB POTTERN, LM GREENBERG, R SCHOENBERG, J SWANSON, GM LIFF, J SCHWARTZ, AG BROWN, LM HOOVER, RN TI VASECTOMY AND PROSTATE-CANCER IN UNITED-STATES BLACKS AND WHITES SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE NEOPLASMS; PROSTATE; VASECTOMY ID HUMAN SEMINAL PLASMA; RISK; MEN AB A large population-based case-control study was carried out to investigate the association between vasectomy and prostate cancer risk in black and in white men in the United States. Study subjects resided in the geographic areas covered by the population-based cancer registries of the Georgia Center for Cancer Statistics, or the Metropolitan Detroit Cancer Surveillance System, or in 10 counties included in the cancer registry of the New Jersey State Health Department. Cases for this study were men aged 40-79 years identified from pathology and outpatient records at hospitals covered by these registries, newly diagnosed with pathologically confirmed prostate cancer between August 1, 1986, and April 30, 1989. Population controls less than age 65 years were selected at periodic intervals by random digit dialing. Older controls were systematically selected (after a random start) from computerized records of the Health Care Finance Administration. A statistically nonsignificant excess risk (odds ratio (OR) = 1.6, 95% confidence interval (Cl) 0.5-4.8) for prostate cancer associated with vasectomy was noted in blacks. Overall, the risk for prostate cancer associated with vasectomy in whites was not elevated (OR = 1.1, 95% Cl 0.8-1.7). An increase in risk was found, however, for white men who had had a vasectomy 20 years or more prior to study (OR = 1.7, 95% Cl 0.9-3.3) or who had had a vasectomy at less than age 35 years (OR = 2.2, 95% Cl 1.0-4.4). For the total study group, the odds ratio associated with men who had a vasectomy 20 or more years prior to study was 1.5 (95% Cl 0.8-2.7), and the odds ratio associated with men who had had a vasectomy at less than age 35 years was 2.0 (95% Cl 1.0-4.0). Further detailed analysis showed that young age at vasectomy (less than age 35 years) was a more important risk factor than was years since vasectomy. C1 NCI,DIETET PROGRAM,BETHESDA,MD 20892. EMORY UNIV,SCH PUBL HLTH,ATLANTA,GA 30322. NEW JERSEY STATE HLTH DEPT,CHRONIC DIS EPIDEMIOL PROGRAM,TRENTON,NJ. MICHIGAN STATE UNIV,CTR CANC,E LANSING,MI 48824. UNIV PITTSBURGH,DEPT CLIN EPIDEMIOL,PITTSBURGH,PA 15260. NR 22 TC 50 Z9 51 U1 0 U2 0 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD FEB 1 PY 1993 VL 137 IS 3 BP 263 EP 269 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KZ184 UT WOS:A1993KZ18400001 PM 8452134 ER PT J AU BRINTON, LA BARRETT, RJ BERMAN, ML MORTEL, R TWIGGS, LB WILBANKS, GD AF BRINTON, LA BARRETT, RJ BERMAN, ML MORTEL, R TWIGGS, LB WILBANKS, GD TI CIGARETTE-SMOKING AND THE RISK OF ENDOMETRIAL CANCER SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE SMOKING; UTERINE NEOPLASMS ID HORMONE-REPLACEMENT THERAPY; POSTMENOPAUSAL WOMEN; NATURAL MENOPAUSE; AGE; ESTROGENS; SMOKERS; PREMENOPAUSAL; ASSOCIATION; NONSMOKERS; ESTRADIOL AB A case-control study involving 405 cases of epithelial endometrial carcinoma (newly diagnosed between 1987 and 1990 in five US areas) and 297 population controls enabled evaluation of risk in relation to detailed smoking characteristics. Cigarette smokers were at a reduced risk of disease, with the effect primarily restricted to women whose diseases were detected postmenopausally (relative risk (RR) = 0.6, 95% confidence interval 0.4-0.9). Among postmenopausal women, current smokers showed the greatest reduction in risk (RR = 0.4, 95% confidence interval 0.2-0.7), with former smokers, including those who had recently stopped, being less affected (RR = 0.8). Other measures of smoking were highly correlated with currency of smoking, but there were no clear patterns of risk with either duration or intensity of smoking. Smoking appeared to reduce risk to the greatest extent in subjects who were multiparous, obese, or nonusers of exogenous hormones, but none of these relations was statistically significant. The results support the notion that smoking reduces the risk of endometrial cancer through extraovarian endogenous hormonal mechanisms, but further studies are needed to clarify why reduced risks are most pronounced among postmenopausal women and those currently exposed to cigarette smoke. C1 WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT OBSTET & GYNECOL,WINSTON SALEM,NC 27103. UNIV CALIF IRVINE,MED CTR,DEPT OBSTET & GYNECOL,IRVINE,CA 92717. PENN STATE UNIV,MILTON S HERSHEY MED CTR,DEPT OBSTET & GYNECOL,HERSHEY,PA 17033. UNIV MINNESOTA,SCH MED,DEPT OBSTET & GYNECOL,MINNEAPOLIS,MN 55455. RUSH MED COLL,DEPT OBSTET & GYNECOL,CHICAGO,IL 60612. RP BRINTON, LA (reprint author), NCI,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,ROOM 443,BETHESDA,MD 20892, USA. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 40 TC 54 Z9 56 U1 0 U2 0 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD FEB 1 PY 1993 VL 137 IS 3 BP 281 EP 291 PG 11 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KZ184 UT WOS:A1993KZ18400003 PM 8452136 ER PT J AU STROUP, DF WHARTON, M KAFADAR, K DEAN, AG AF STROUP, DF WHARTON, M KAFADAR, K DEAN, AG TI EVALUATION OF A METHOD FOR DETECTING ABERRATIONS IN PUBLIC-HEALTH SURVEILLANCE DATA SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE EPIDEMIOLOGIC METHODS; PUBLIC HEALTH SURVEILLANCE ID UNITED-STATES; BOOTSTRAP; DISEASES AB The detection of unusual patterns in routine public health surveillance data on diseases and injuries presents an important challenge to health workers interested in early identification of epidemics or clues to important risk factors. Each week, state health departments report the numbers of cases of about 50 notifiable diseases to the Centers for Disease Control and Prevention, and these reports are published weekly in the Morbidity and Mortality Weekly Report. A new analytic method and a horizontal bar graph were introduced in July 1989 to facilitate easy identification of unusual numbers of reported cases. Evaluation of the statistical properties of this method indicates that the results are fairly robust to nonnormality and serial correlation of the data. An epidemiologic evaluation of the method after the first 6 months showed that it is useful for detection of specific types of aberrations in public health surveillance. C1 CTR DIS CONTROL & PREVENT,NATL CTR PREVENT SERV,ATLANTA,GA. NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. RP STROUP, DF (reprint author), CTR DIS CONTROL & PREVENT,EPIDEMIOL PROGRAM OFF,DIV SURVEILLANCE & EPIDEMIOL,MAIL STOP C08,ATLANTA,GA 30333, USA. NR 21 TC 41 Z9 43 U1 0 U2 2 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD FEB 1 PY 1993 VL 137 IS 3 BP 373 EP 380 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KZ184 UT WOS:A1993KZ18400012 PM 8452145 ER PT J AU BENYA, RV METZ, DC HIJAZI, YM FISHBEYN, VA PISEGNA, JR JENSEN, RT AF BENYA, RV METZ, DC HIJAZI, YM FISHBEYN, VA PISEGNA, JR JENSEN, RT TI FINE NEEDLE ASPIRATION CYTOLOGY OF SUBMUCOSAL NODULES IN PATIENTS WITH ZOLLINGER-ELLISON SYNDROME SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Article ID MULTIPLE ENDOCRINE NEOPLASIA; GASTRIC ARGYROPHIL CARCINOIDOSIS; ENDOSCOPIC ULTRASONOGRAPHY; DIAGNOSIS; GASTROESOPHAGEAL; MALIGNANCIES; ASSOCIATION; BIOPSY; TUMOR; TRACT AB Submucosal nodules are often encountered during investigations of the upper gastrointestinal (GI) tract. This is particularly true in diseases resulting in chronic hypergastrinemia, such as Zollinger-Ellison syndrome (ZES), in which submucosal gastric and duodenal lesions can occur. Forceps biopsy of submucosal lesions often yields only normal mucosa; however, fine needle aspiration cytology (FNAC) has recently been described as having high diagnostic accuracy for submucosal tumors. Therefore, we prospectively studied the use of FNAC in 43 patients with ZES. Overall, 33% of patients with ZES had nodules. In patients with the sporadic form of ZES, submucosal nodules were found in 18%, whereas submucosal nodules were found in 80% of patients who had ZES in conjunction with multiple endocrine neoplasia type I (MEN-I). FNAC identified 11/12 (92%) of the neuroendocrine tumors, and identified another 8/9 as non-neuroendocrine. Jumbo forceps biopsy was performed on 18 nodules and diagnosed one neuroendocrine tumor. Subsequently, 11 of these nodules were found to possess neuroendocrine tumor; thus only 1/11 (9%) neuroendocrine tumors removed were accurately identified by jumbo forceps biopsy. Of the first 14 nodules, sufficient tissue was left after biopsy to permit snare polypectomy on 12 nodules. Four nodules were found to contain neuroendocrine tumor. Snare polypectomy resulted in a duodenal perforation that required surgery in one patient, and thus was not performed on the final seven nodules. We conclude that 1) upper GI tract submucosal nodules are common in patients with ZES, although neuroendocrine tumor is common only in those patients with ZES and MEN-I; 2) FNAC can accurately diagnose submucosal neuroendocrine tumors in patients with ZES; 3) jumbo biopsy is not helpful in the evaluation of these submucosal nodules; and 4) snare polypectomy can result in duodenal perforation, and thus should not be routinely performed. C1 NIH,DIGEST DIS BRANCH,BLDG 10,9C-103,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIH,OBSTET & GYNAECOL LAB,BETHESDA,MD 20892. NR 25 TC 12 Z9 12 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD FEB PY 1993 VL 88 IS 2 BP 258 EP 265 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA KL615 UT WOS:A1993KL61500019 PM 8093826 ER PT J AU MCKEOWN, LP WILLIAMS, SB SHAFER, B MURRAY, N GRALNICK, HR AF MCKEOWN, LP WILLIAMS, SB SHAFER, B MURRAY, N GRALNICK, HR TI ALPHA-GRANULE PROTEINS IN TYPE-I VONWILLEBRANDS DISEASE SO AMERICAN JOURNAL OF HEMATOLOGY LA English DT Article DE FIBRINOGEN; VWD; VWF ID VIII-RELATED ANTIGEN; THROMBIN-STIMULATED PLATELETS; IMMUNOFLUORESCENT LOCALIZATION; HUMAN MEGAKARYOCYTES; FIBRINOGEN; THROMBOSPONDIN; THROMBASTHENIA; RELEASE AB Platelet von Willebrand factor (vWf) is located in the alpha granules. Individuals with type I von Willebrand's disease (vWd) with prolonged bleeding times are best discriminated from those who have normal bleeding times by the normal level of platelet vWf ristocetin cofactor activity (vWf activity) and, to a lesser extent, by their platelet vWf antigen content. We have studied the content of adhesive proteins and platelet factor-4 (PF-4), and beta-thromboglobulin (betaTG) in the platelet alpha granules of types I and III vWd patients to determine if other alterations in alpha granule contents or proteins occur in vWd. We found that type I vWd patients with prolonged or normal bleeding times could not be differentiated on the basis of their platelet levels of betaTG, PF-4, fibronectin, or fibrinogen. The levels of the alpha granule constituents in the type I vWd patient were similar to normal except for the platelet fibrinogen concentration. Patients with type I vWd, regardless of the level of platelet vWf activity or antigen, had increased levels of platelet fibrinogen. The patients with type III vWd who had undetectable levels of platelet and plasma vWf also had increased levels of platelet fibrinogen. In our study we could not attribute the variation in the platelet vWf activity and antigen in type I vWd to the size of the alpha granule pool as determined by the measurement of other alpha granule proteins. The mechanism(s) of increased platelet fibrinogen in these vWd patients is at present unknown. C1 NIH,CTR CLIN,HEMATOL SERV,9000 ROCKVILLE PIKE,BLDG 10,RM 2C390,BETHESDA,MD 20892. NR 20 TC 4 Z9 4 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0361-8609 J9 AM J HEMATOL JI Am. J. Hematol. PD FEB PY 1993 VL 42 IS 2 BP 158 EP 161 DI 10.1002/ajh.2830420203 PG 4 WC Hematology SC Hematology GA KL610 UT WOS:A1993KL61000002 PM 8438875 ER PT J AU BLAIR, A LINOS, A STEWART, PA BURMEISTER, LF GIBSON, R EVERETT, G SCHUMAN, L CANTOR, KP AF BLAIR, A LINOS, A STEWART, PA BURMEISTER, LF GIBSON, R EVERETT, G SCHUMAN, L CANTOR, KP TI EVALUATION OF RISKS FOR NON-HODGKINS-LYMPHOMA BY OCCUPATION AND INDUSTRY EXPOSURES FROM A CASE-CONTROL STUDY SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article DE OCCUPATIONAL RISK ESTIMATION; CANCER; PRINTERS; DRY CLEANERS; BENZENE; METAL WORKING; NHL; NONAGRICULTURAL EXPOSURES; EMBALMERS ID SOFT-TISSUE SARCOMA; UNITED-STATES; MALIGNANT-LYMPHOMAS; ORGANIC-SOLVENTS; CANCER MORTALITY; WORKERS; LEUKEMIA; PATTERNS; FACILITY; COHORT AB The etiology of non-Hodgkin's lymphoma (NHL) is not well understood. To develop hypotheses on causes of this tumor, data from a population-based case-control interview study of 1,867 white men (622 cases and 1,245 controls) in Iowa and Minnesota conducted during 1980-1983 were examined. Subjects, or their next of kin, were interviewed to obtain information on agricultural exposures, work history, medical conditions, and family history. This analysis focuses on risks of NHL by occupation, by industry, and by selected exposures. Although many comparisons were made, few significant associations were observed. Small numbers and limitations in exposure assessment, however, would tend to reduce opportunities to detect associations. The strongest finding was with various occupations that work in metals and metal products. The analysis by exposure estimates also uncovered a significant association with metals, but risks did not increase with estimated intensity of exposure. Slightly elevated risks were also noted among persons employed as painters and construction workers, agricultural and forestry workers, printers and typesetters, funeral directors and embalmers, and dry cleaners. Although the overall risks for benzene and other solvents were small, they increased slightly with level of assigned exposure. Although some associations may be due to chance, several of these occupations and industries have been linked to lymphoma in other investigations and deserve further attention. C1 ORLANDO REG MED CTR INC,DEPT INTERNAL MED,ORLANDO,FL. UNIV ATHENS,DEPT EPIDEMIOL,ATHENS,GREECE. UNIV IOWA,DEPT PREVENT MED,IOWA CITY,IA 52242. UNIV MINNESOTA,DEPT EPIDEMIOL,MINNEAPOLIS,MN 55455. RP BLAIR, A (reprint author), NCI,ENVIRONM EPIDEMIOL BRANCH,EXECUT PLAZA N,ROOM 418,ROCKVILLE,MD 20892, USA. NR 45 TC 106 Z9 107 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0271-3586 J9 AM J IND MED JI Am. J. Ind. Med. PD FEB PY 1993 VL 23 IS 2 BP 301 EP 312 DI 10.1002/ajim.4700230207 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KJ713 UT WOS:A1993KJ71300006 PM 8427258 ER PT J AU POLIS, MA DESMET, MD BAIRD, BF MELLOW, S FALLOON, J DAVEY, RT KOVACS, JA PALESTINE, AG NUSSENBLATT, RB MASUR, H LANE, HC AF POLIS, MA DESMET, MD BAIRD, BF MELLOW, S FALLOON, J DAVEY, RT KOVACS, JA PALESTINE, AG NUSSENBLATT, RB MASUR, H LANE, HC TI INCREASED SURVIVAL OF A COHORT OF PATIENTS WITH ACQUIRED-IMMUNODEFICIENCY-SYNDROME AND CYTOMEGALOVIRUS RETINITIS WHO RECEIVED SODIUM PHOSPHONOFORMATE (FOSCARNET) SO AMERICAN JOURNAL OF MEDICINE LA English DT Article ID IMMUNE-DEFICIENCY-SYNDROME; MUCOCUTANEOUS HERPES-SIMPLEX; AIDS-RELATED COMPLEX; VIRUS RETINITIS; CONTROLLED TRIAL; THERAPY; ZIDOVUDINE; INFECTION; GANCICLOVIR AB PURPOSE: To evaluate the impact of foscarnet on the longevity of persons with human immunodeficiency virus, type 1 (HIV-1) infection and cytomegalovirus (CMV) retinitis. PATIENTS AND METHODS: A cohort of 24 patients with acquired immunodeficiency syndrome (AIDS) and CMV retinitis received sodium phosphonoformate (foscarnet) as part of a controlled efficacy trial at the National Institutes of Health. Foscarnet was continued for as long as it was tolerated. Antiretroviral therapy was given to the patients as tolerated. Long-term follow-up was available on all patients. RESULTS: Seventeen patients received zidovudine during or after receiving foscarnet, 2 patients received dideoxyinosine, 2 patients zidovudine and dideoxyinosine, and 3 patients received no specific antiretroviral agent. Patients received foscarnet for a mean of 6.2 months (median, 4 months: range, 10 days to 22 months). Ten patients required a change to ganciclovir therapy at some time after receiving foscarnet. The median time from the diagnosis of CMV retinitis until death was 13.5 months (range, 3 to 34 months). Patients lived longer than untreated or ganciclovir-treated historical controls with AIDS and CMV retinitis. There was no difference in the survival of patients treated with foscarnet at the time of diagnosis and those patients treated with foscarnet only after progression of their CMV retinitis. C1 NEI,IMMUNOL LAB,BETHESDA,MD 20892. NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT CRIT CARE MED,BETHESDA,MD 20892. RP POLIS, MA (reprint author), NIAID,IMMUNOREGULAT LAB,BLDG 10,ROOM 11B13,BETHESDA,MD 20892, USA. OI Polis, Michael/0000-0002-9151-2268 NR 29 TC 46 Z9 46 U1 0 U2 1 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD FEB PY 1993 VL 94 IS 2 BP 175 EP 180 DI 10.1016/0002-9343(93)90180-W PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA KL407 UT WOS:A1993KL40700010 PM 8381583 ER PT J AU READ, JS KLEBANOFF, MA AF READ, JS KLEBANOFF, MA TI SEXUAL INTERCOURSE DURING PREGNANCY AND PRETERM DELIVERY - EFFECTS OF VAGINAL MICROORGANISMS SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article DE SEXUAL INTERCOURSE; PREMATURITY; MICROBIOLOGY ID PLACEBO-CONTROLLED TRIAL; UREAPLASMA-UREALYTICUM; PREMATURE DELIVERY; COITUS; ERYTHROMYCIN AB OBJECTIVE: Our aim was to investigate the influence of vaginal colonization with specific microorganisms on the relationship between sexual intercourse during pregnancy and preterm delivery. STUDY DESIGN: As part of a multicenter, prospective study interviews and physical examinations were conducted with and genital cultures were obtained from women seeking prenatal care from 23 to 26 weeks' gestation. At 31 to 36 weeks interviews were conducted with a randomly selected sample of these patients. RESULTS: Frequent intercourse (defined a priori as once per week or more) at 23 to 26 weeks was associated with a significantly reduced risk of subsequent preterm delivery in women without Trichomonas vaginalis, Mycoplasma hominis, or bacterial vaginosis, possibly because of the relative health and lack of complications in the pregnancies of those women engaging in sexual intercourse. Frequent intercourse was not significantly associated with preterm delivery in women with T. vaginalis, M. hominis, or bacterial vaginosis. Neither T. vaginalis, M. hominis, nor bacterial vaginosis was associated with preterm delivery among women with infrequent intercourse at 23 to 26 weeks. However, T vaginalis and M. hominis were risk factors for preterm delivery among those with frequent intercourse. CONCLUSIONS: Frequent sexual intercourse by itself is not associated with an increased risk of preterm birth. However, women who are colonized with specific microorganisms and who engage in frequent intercourse are at increased risk of preterm delivery. RP READ, JS (reprint author), NICHHD,DESPR,6100 EXECUT BLVD,ROOM 7B03,BETHESDA,MD 20892, USA. FU NICHD NIH HHS [HD-3-2832, HD-3-2833, HD-3-2834] NR 25 TC 56 Z9 58 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-9378 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD FEB PY 1993 VL 168 IS 2 BP 514 EP 519 PG 6 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA KN840 UT WOS:A1993KN84000011 PM 8438920 ER PT J AU BERMAN, JJ MOORE, GW ONEILL, TP LIEBELT, AG SAFFIOTTI, U AF BERMAN, JJ MOORE, GW ONEILL, TP LIEBELT, AG SAFFIOTTI, U TI REGISTRY OF EXPERIMENTAL CANCERS OF THE NATIONAL CANCER INSTITUTE - A DATABASE RESOURCE FOR CANCER-RESEARCH SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article AB The National Cancer Institute established the Registry of Experimental Cancers in March 1970. This registry consists of a permanent collection of pathological materials on spontaneous and induced lesions in laboratory animals that includes histological slides, paraffin blocks, autopsy findings, pathological diagnoses, photographs, and experimental records. The material presently is composed of approximately 60,000 consecutive records and is a valuable resource for researchers interested in tumors and other lesions arising spontaneously or from specific induction protocols in experimental animals. The entire registry database was transferred to an object-oriented database that permits registry staff to write programs for the different data field objects, thus customizing searches and other database functions. Twenty-seven animal species are represented and a total of 6,496 diagnostic entities and 1,106 treatment and control protocols are listed Archival material may be retrieved for analysis of molecular markers. C1 JOHNS HOPKINS MED INST,DEPT PATHOL,BALTIMORE,MD 21205. NCI,EXPTL PATHOL LAB,BETHESDA,MD 20892. RP BERMAN, JJ (reprint author), UNIV MARYLAND,SCH MED,VA MED CTR,DEPT PATHOL,SURG PATHOL SECT,LAB SERV,10 N GREENE ST,BALTIMORE,MD 21201, USA. NR 3 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD FEB PY 1993 VL 142 IS 2 BP 351 EP 352 PG 2 WC Pathology SC Pathology GA KL858 UT WOS:A1993KL85800001 PM 8434635 ER PT J AU CHOU, CL KNEPPER, MA AF CHOU, CL KNEPPER, MA TI INVITRO PERFUSION OF CHINCHILLA THIN LIMB SEGMENTS - UREA AND NACL PERMEABILITIES SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE DESCENDING LIMB; ASCENDING LIMB; HENLES LOOP ID MEDULLARY COLLECTING DUCT; LONG-LOOPED NEPHRON; CENTRAL CORE MODEL; HUMAN RED-CELLS; DESCENDING-LIMB; HENLES LOOP; ASCENDING LIMB; WATER TRANSPORT; ION CONDUCTANCE; UPPER PORTION AB We measured the urea and NaCl permeabilities (P(urea) and P(NaCl), respectively) of the following nephron segments from chinchilla: the upper part of the long-loop descending limb (from outer medulla, LDL(u)), the middle part of the long-loop descending limb (from outer 30% of the inner medulla, LDL(m)), the lower part of the long-loop descending limb (from deep inner medulla, LDL(l)), and the thin ascending limb (from deep inner medulla, ATL). We found that P(urea) (X 10(-5) CM/s) was relatively low in the LDL(u) (3.3), but that the value was larger in the inner medullary thin descending limb (16.8 for LDL(m) and 47.6 for LDL(l)). The ATL had an even higher value (I 70). Phloretin, 0.25 mM, added to the peritubular bath had no effect on P(urea) of these segments, suggesting that the rapid transport rate is not due to a phloretin-sensitive facilitated transport pathway like that seen in the inner medullary collecting duct. P(NaCl) (x 10(-5) CM/s) also increased with distance along the length of the thin descending limb (LDL(u), 11.7; LDL(m), 41.2; LDL(l), 98.4; and ATL, 321). Calculations from NaCl dilution potential measurements showed that LDL(u) was Na+ permselective, whereas LDL(l) and ATL were Cl- permselective. High solute permeabilities in the inner medullary thin descending limb contradict a major requirement of the passive model of urinary concentration developed previously (J. P. Kokko and F. C. Rector, Jr. Kidney Int. 2: 214-223, 1972; and J. L. Stepohenson. Kidney Int. 2: 85-94, 1972). RP CHOU, CL (reprint author), NHLBI,KIDNEY & ELECTROLYTE METAB LAB,BETHESDA,MD 20892, USA. NR 38 TC 53 Z9 53 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1993 VL 264 IS 2 BP F337 EP F343 PN 2 PG 7 WC Physiology SC Physiology GA KN681 UT WOS:A1993KN68100112 PM 8447443 ER PT J AU HEINEMAN, FW BALABAN, RS AF HEINEMAN, FW BALABAN, RS TI EFFECTS OF AFTERLOAD AND HEART-RATE ON NAD(P)H REDOX STATE IN THE ISOLATED RABBIT HEART SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE OXIDATIVE PHOSPHORYLATION; MYOCARDIAL OXYGEN CONSUMPTION; MYOCARDIAL METABOLISM; FLUORESCENCE ID NADH FLUORESCENCE; OXIDATIVE-PHOSPHORYLATION; RESPIRATORY CONTROL; CALCIUM-TRANSPORT; PERFUSED HEART; P-31 NMR; MITOCHONDRIA; METABOLISM; SYSTEM; INVIVO AB NAD(P)H redox state was monitored using surface fluorescence in isolated, normothermic, working rabbit hearts under conditions of limited substrate (glucose alone) and abundant substrate (glucose + lactate). To alter work, afterload was varied between 75 and 150 cmH2O or heart rate was increased in steps until no further increase in myocardial oxygen consumption (MVO2) occurred. Alterations in afterload did not cause a significant change in NAD(P)H fluorescence. Progressive increases in heart rate did not alter NAD(P)H emission until MVO2 began to decline (approximately 300 beats/min), ventricular performance decompensated, and there was evidence of ischemia, at which time NAD(P)H fluorescence increased. Although the addition of 3 mM lactate to the perfusate resulted in a rapid increase in NAD(P)H fluorescence, NAD(P)H fluorescence still did not respond to altered workload. The results suggest that NAD(P)H redox state is not the primary stimulus for increased myocardial respiration secondary to tachycardia or afterload. However, despite increased rates of cardiac work, NAD(P)H was maintained at a relatively stable level, suggesting that reducing equivalent supply to the electron transport chain increases in parallel with increased MVO2. RP HEINEMAN, FW (reprint author), NHLBI,CARDIAC ENERGET LAB,BLDG 1-B3-07,BETHESDA,MD 20892, USA. RI Balaban, Robert/A-7459-2009 OI Balaban, Robert/0000-0003-4086-0948 NR 29 TC 85 Z9 86 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1993 VL 264 IS 2 BP H433 EP H440 PN 2 PG 8 WC Physiology SC Physiology GA KN681 UT WOS:A1993KN68100022 PM 8447459 ER PT J AU HOLLENBERG, SM SHELHAMER, JH CUNNION, RE AF HOLLENBERG, SM SHELHAMER, JH CUNNION, RE TI TACHYPHYLAXIS TO THE VASOPRESSOR EFFECTS OF ENDOTHELIN IN RAT AORTIC RINGS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE ENDOTHELIN-RECEPTOR COMPLEX; SYSTEMIC VASCULAR RESISTANCE ID VASCULAR SMOOTH-MUSCLE; VASOCONSTRICTOR ENDOTHELIN; CELLS; VASODILATOR; PEPTIDE; CALCIUM; FAMILY; CA-2+ AB Endothelin-1 (ET-1), a potent vasoconstrictor peptide released by endothelial cells, binds with high affinity to surface receptors and is highly resistant to dissociation. We observed tachyphylaxis to the pressor effects of a second application of ET-1 in rat aortic rings and investigated the mechanism of this effect. Developed tension increased progressively with doses of ET-1 ranging from 1 to 500 nM (P < 0.001), and tensions with rechallenge were correspondingly decreased (P < 0.001). In response to 500 nM ET-1, tension increased 1,599 +/- 72 (SE) mg/mg ring wt. Rechallenge with 500 nM ET-1 led to contraction of only 33 +/- 40 mg/mg ring wt. Tachyphylaxis was seen up to 6 h after initial challenge. Pretreatment with nicardipine, lidoflazine, nitroglycerin, and sphingosine did not affect tachyphylaxis. Pretreatment with 500 muM dansylcadaverine (an inhibitor of endothelin internalization) markedly inhibited ET-1-induced contraction and also inhibited tachyphylaxis to ET-1. Further studies with radiolabeled ET-1 suggested that subsequent ET-1 binding is markedly decreased after an initial ET-1 challenge. Dansylcadaverine inhibited ET-1 internalization and also inhibited the decreased binding seen with ET-1 rechallenge. Rat aortic rings demonstrate tachyphylaxis to the pressor effect of a second dose of ET-1. The mechanism appears to be related to binding and subsequent internalization of endothelin-receptor complexes. This effect suggests a possible mechanism for sustained decreases in systemic vascular resistance. RP HOLLENBERG, SM (reprint author), NIH,CTR CLIN,DEPT CRIT CARE MED,BLDG 10,ROOM 7-D-43,BETHESDA,MD 20892, USA. NR 21 TC 11 Z9 11 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1993 VL 264 IS 2 BP H352 EP H356 PN 2 PG 5 WC Physiology SC Physiology GA KN681 UT WOS:A1993KN68100009 PM 8447451 ER PT J AU HOLLENBERG, SM CUNNION, RE ZIMMERBERG, J AF HOLLENBERG, SM CUNNION, RE ZIMMERBERG, J TI NITRIC-OXIDE SYNTHASE INHIBITION REVERSES ARTERIOLAR HYPORESPONSIVENESS TO CATECHOLAMINES IN SEPTIC RATS SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Note DE SEPSIS; NOREPINEPHRINE; N(G)-MONOMETHYL-L-ARGININE; VIDEOMICROSCOPY ID VASCULAR SMOOTH-MUSCLE; TUMOR-NECROSIS-FACTOR; ESCHERICHIA-COLI ENDOTOXIN; L-ARGININE; CREMASTER MUSCLE; RESPONSIVENESS; CONTRACTILITY; IMPAIRMENT; DEPRESSION; RESPONSES AB Induction of nitric oxide synthase by cytokines has been hypothesized as a mechanism of the hyporesponsiveness to catecholamines that occurs in clinical septic shock. We measured responses of resistance arterioles in rat cremaster muscle to topically suffused norepinephrine in vivo with the use of image-shearing videomicroscopy. Rats made septic by cecal ligation and puncture were compared with controls that underwent sham ligation. The norepinephrine concentration-response curve was shifted to the right in septic rats [50% effective concentration (EC50) 9.1 +/- 5.4 vs. 0.10 +/- 0.02 muM, P < 0.05]. Contractions at doses of 10(-9), 10(-8), and 10(-7) M norepinephrine were 26, 41, and 38%, respectively, of sham controls. Superfusion of the muscle with the nitric oxide synthase inhibitor N(G)-monomethyl-L-arginine at 100 muM restored the arteriolar responsiveness of the septic rats (EC50 0.14 +/- 0.07 vs. 6.8 +/-3.1 muM, P < 0.05). This effect was reversed with superfusion of excess (1 mM) L-arginine. These experiments demonstrate impaired vasoconstriction in response to norepinephrine in resistance arterioles of septic rats in vivo. N(G)-monomethyl-L-arginine reversed this hyporesponsiveness, implying that nitric oxide synthase may mediate the decreased catecholamine responsiveness associated with sepsis. C1 NICHHD,THEORET & PHYS BIOL LAB,BETHESDA,MD 20892. RP HOLLENBERG, SM (reprint author), NICHHD,CTR CLIN,DEPT CRIT CARE MED,BLDG 10,RM 7D-43,BETHESDA,MD 20892, USA. NR 29 TC 113 Z9 114 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1993 VL 264 IS 2 BP H660 EP H663 PN 2 PG 4 WC Physiology SC Physiology GA KN681 UT WOS:A1993KN68100053 PM 7680541 ER PT J AU EVERSON, CA WEHR, TA AF EVERSON, CA WEHR, TA TI NUTRITIONAL AND METABOLIC ADAPTATIONS TO PROLONGED SLEEP-DEPRIVATION IN THE RAT SO AMERICAN JOURNAL OF PHYSIOLOGY LA English DT Article DE METABOLISM; ENERGY EXPENDITURE; BODY WEIGHT REGULATION; FOOD INTAKE; CLINICAL CHEMISTRY; HEMATOLOGY; UNDERNUTRITION; MALNUTRITION; PROTEIN-ENERGY MALNUTRITION ID PROTEIN; MALNUTRITION AB To understand how and why sleep deprivation, is physically harmful, we explored the possible causal relationship between its two main effects, 1) negative energy balance and 2) a composite of symptoms that resemble protein malnutrition, both of which occur despite increased food consumption. We provided balanced diets augmented with either protein or calories (by increased fat content) to freely moving rats. Interactions between sleep deprivation symptoms and energy and protein supplies were assessed from measurements of body weight regulation, consumption of macronutrients, clinical chemist and hematology profiles, and physical appearance. The results indicate that sleep deprivation causes malnutrition, which is secondary to increased energy expenditure. Even though food consumption remained normal in sleep-deprived rats fed a diet of high protein-to-calorie ratio, body weight loss was more than 16% of baseline, development of skin lesions was hastened, and longevity was shortened by 40% compared with sleep-deprived rats fed the calorie-augmented diet. Food consumption of the calorie-fed rats was lower during baseline than that of the protein-fed group but during sleep deprivation increased to amounts 250% of normal without net body weight gain. Despite a fat-laden diet the calorie-fed hyperphagic group did not have abnormal levels of plasma cholesterol, triglycerides, or glucose, indicating accelerated turnover of nutrients. As would be consistent with calorie malnutrition, pronounced clinical chemistry or hematological abnormalities were not found in any group. Beneficial effects of the calorie-augmented diet are attributed to 1) caloric density of fat, 2) induction of hyperphagia, and 3) efficiency of utilization of fat. We conclude that diet composition interacts strongly with sleep deprivation, affecting the time course and development of pathologies, whereas it exerted negligible influence on body weight regulation under normal conditions. RP EVERSON, CA (reprint author), NIMH,CPB,BLDG 10,RM 4S239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Biguzzi, Felipe/E-4724-2015 NR 41 TC 50 Z9 51 U1 2 U2 5 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0002-9513 J9 AM J PHYSIOL JI Am. J. Physiol. PD FEB PY 1993 VL 264 IS 2 BP R376 EP R387 PN 2 PG 12 WC Physiology SC Physiology GA KN681 UT WOS:A1993KN68100076 PM 8447493 ER PT J AU LEIBENLUFT, E FIERO, PL BARTKO, JJ MOUL, DE ROSENTHAL, NE AF LEIBENLUFT, E FIERO, PL BARTKO, JJ MOUL, DE ROSENTHAL, NE TI DEPRESSIVE SYMPTOMS AND THE SELF-REPORTED USE OF ALCOHOL, CAFFEINE, AND CARBOHYDRATES IN NORMAL VOLUNTEERS AND 4 GROUPS OF PSYCHIATRIC OUTPATIENTS SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID SEASONAL AFFECTIVE-DISORDER; CLINICAL IMPLICATIONS; ANXIETY AB Objective: The authors examined the relationship between depressive symptoms and the self-reported use of alcohol, carbohydrates, and caffeine in normal volunteers and four groups of psychiatric outpatients. Method: Outpatients and normal volunteers were given a questionnaire asking about their use of each of the three substances in response to each of the 14 depressive symptoms on the Hamilton Rating Scale for Depression. They also rated bow much each substance improved each symptom. Twenty-six normal volunteers, 35 patients with major depression, 117 patients with seasonal affective disorder, 16 patients with alcohol dependence, and 24 patients with comorbid primary depression and secondary alcohol dependence completed the questionnaire. Test-retest reliability was established. Analysis of variance and stepwise multivariate discriminant function analyses were used to determine if diagnostic groups differed in the reported use and effect of each of the three substances. Results: The responses concerning use and effect of alcohol of patients with alcohol dependence with or without depression were indistinguishable from each other. The responses of the patient groups regarding caffeine and carbohydrate use did not differ from each other, but all differed significantly from the responses of normal volunteers. Discriminant function analysis distinguished alcoholics from nonalcoholics in the relationship between drinking and the symptoms of anger and anhedonia. Conclusions: The relationship between symptoms and substance use varied depending on the substance. Alcoholics without depression were as likely to report drinking in response to depressive symptoms as were those who had had depression. Patients of all diagnostic groups were more likely than normal volunteers to report using caffeine and carbohydrates in response to depressive symptoms. RP LEIBENLUFT, E (reprint author), NIMH,CLIN PSYCHOBIOL BRANCH,BLDG 10-4S-239,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 40 TC 50 Z9 51 U1 0 U2 7 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD FEB PY 1993 VL 150 IS 2 BP 294 EP 301 PG 8 WC Psychiatry SC Psychiatry GA KJ624 UT WOS:A1993KJ62400017 PM 8422081 ER PT J AU TANCER, ME STEIN, MB BLACK, B UHDE, TW AF TANCER, ME STEIN, MB BLACK, B UHDE, TW TI BLUNTED GROWTH-HORMONE RESPONSES TO GROWTH HORMONE-RELEASING FACTOR AND TO CLONIDINE IN PANIC DISORDER SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID DEPRESSION AB Blunted growth hormone (GH) responses to growth hormone-releasing factor (GH-RF) and clonidine have been reported in patients with panic disorder. In this study GH-RF and clonidine were administered to 13 patients with panic disorder and 20 healthy volunteers. Compared to the normal subjects, the patients with panic disorder had significantly blunted GH responses after both GH-RF and clonidine. C1 NIMH,BIOL PSYCHIAT BRANCH,ANXIETY & AFFECT DISORDERS SECT,BLDG 10,BETHESDA,MD 20892. NR 11 TC 30 Z9 30 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD FEB PY 1993 VL 150 IS 2 BP 336 EP 337 PG 2 WC Psychiatry SC Psychiatry GA KJ624 UT WOS:A1993KJ62400027 PM 8422091 ER PT J AU WINDSOR, RA LI, CQ LOWE, JB PERKINS, LL ERSHOFF, D GLYNN, T AF WINDSOR, RA LI, CQ LOWE, JB PERKINS, LL ERSHOFF, D GLYNN, T TI THE DISSEMINATION OF SMOKING CESSATION METHODS FOR PREGNANT-WOMEN - ACHIEVING THE YEAR 2000 OBJECTIVES SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID MATERNAL SMOKING; EDUCATION-PROGRAM; RANDOMIZED TRIAL; COST OUTCOMES; BIRTH-WEIGHT; HEALTH; CARE; HMO; INTERVENTIONS AB The smoking prevalence rate among adult women and pregnant women has decreased only 0.3 to 0.5% per year since 1969. Without a nationwide dissemination of efficacious smoking cessation methods based on these trends, by the year 2000 the smoking prevalence among pregnant women will be approximately 18%. This estimate is well above the US Department of Health and Human Services Year 2000 Objective of 10%. The US dissemination of tested smoking cessation methods could help an additional 12 900 to 155 000 pregnant smokers annually and 600 000 to 1 481 000 cumulatively to quit smoking during the 1990s. Dissemination could help achieve 31 to 78% of the Year 2000 Objectives for pregnancy smoking prevalence. (With dissemination, at best a 15% smoking prevalence during pregnancy, rather than the 10% objective, is likely to be observed.) Our results confirm a well-documented need for a national campaign to disseminate smoking cessation methods. C1 UNIV ALABAMA,SCH MED,OFF EDUC RES & DEV,401 CHSB 19TH ST S,BIRMINGHAM,AL 35294. KAISER PERMANENTE,LOS ANGELES,CA. UNIV QUEENSLAND,CANC PREVENT RES CTR,ST LUCIA,QLD 4067,AUSTRALIA. NCI,BETHESDA,MD 20892. UNIV ALABAMA,SCH PUBL HLTH,BIRMINGHAM,AL 35294. OI Lowe, John/0000-0003-1222-2295 NR 48 TC 30 Z9 30 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 173 EP 178 DI 10.2105/AJPH.83.2.173 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300002 PM 8427318 ER PT J AU WINDSOR, RA LOWE, JB PERKINS, LL SMITHYODER, D ARTZ, L CRAWFORD, M AMBURGY, K BOYD, NR AF WINDSOR, RA LOWE, JB PERKINS, LL SMITHYODER, D ARTZ, L CRAWFORD, M AMBURGY, K BOYD, NR TI HEALTH-EDUCATION FOR PREGNANT SMOKERS - ITS BEHAVIORAL IMPACT AND COST-BENEFIT SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID SMOKING CESSATION PROGRAM; SELF-REPORTED SMOKING; MATERNAL SMOKING; RANDOMIZED TRIAL; WOMEN; COTININE; VALIDATION; HMO AB Objectives. A randomized trial (the Birmingham Trial II) was conducted to evaluate the behavioral impact of health education methods among 814 female smokers at four public health maternity clinics. Methods. Four hundred patients were randomly assigned to an Experimental (E) Group, and 414 were assigned to a Control (C) Group. Self-reports and saliva cotinine tests confirmed smoking status at the first visit, at midpregnancy, and at end of pregnancy. Results. The E Group exhibited a 14.3% quit rate and the C Group an 8.5% quit rate. A Historical Comparison (Cunderbar) Group exhibited a 3.0% quit rate. Black E and C Group patients had higher quit rates than White E and C Group patients. A cost-benefit analysis found cost-to-benefit ratios of $1:$6.72 (low estimate) and $1:$17.18 (high estimate) and an estimated savings of $247 296 (low estimate) and $699 240 (high estimate). Conclusion. Health education methods are efficacious and cost beneficial for pregnant smokers in public health maternity clinics. C1 ALABAMA DEPT PUBL HLTH,MONTGOMERY,AL. STATE WASHINGTON DEPT HLTH,OLYMPIA,WA. NCI,BETHESDA,MD 20892. UNIV ALABAMA,SCH PUBL HLTH,BIRMINGHAM,AL 35294. UNIV ALABAMA,SCH MED,BIRMINGHAM,AL 35233. UNIV QUEENSLAND,CANC PREVENT RES CTR,ST LUCIA,QLD 4067,AUSTRALIA. OI Lowe, John/0000-0003-1222-2295 FU NCI NIH HHS [R01CA41648] NR 38 TC 178 Z9 179 U1 0 U2 1 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 201 EP 206 DI 10.2105/AJPH.83.2.201 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300007 PM 8427323 ER PT J AU ZHANG, J RATCLIFFE, JM AF ZHANG, J RATCLIFFE, JM TI PATERNAL SMOKING AND BIRTH-WEIGHT IN SHANGHAI SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID LOW-BIRTH-WEIGHT; PASSIVE SMOKING; MATERNAL SMOKING; PREGNANCY; ASSOCIATION; EXPOSURE; WOMEN AB Objectives. Although maternal active smoking has been established to be associated with fetal growth retardation, evidence of an effect of environmental tobacco smoke exposure on birthweight is still limited and inconclusive. This study addressed the relationship between prenatal environmental tobacco smoke exposure and birthweight and fetal growth retardation in Shanghai, China. Methods. Data on 1785 full-term live-born normal infants of nonsmoking mothers were used from the Shanghai Birth Defects and Perinatal Death Monitoring conducted between October 1986 and September 1987. Environmental tobacco smoke exposure was defined as exposure to paternal smoking. Results. Infants with environmental tobacco smoking exposure were, on average, 30 g lower in birthweight than nonexposed infants, after adjustment for gestational age, parity, maternal age, and occupation. Conclusion. Consistent with previous research, this study suggests that environmental tobacco smoking exposure may have a modestly adverse effect on birthweight. C1 UNIV N CAROLINA,CAROLINA POPULAT CTR,CHAPEL HILL,NC 27599. NIEHS,EPIDEMIOL BRANCH,DIV BIOMETRY & RISK ASSESSMENT,RES TRIANGLE PK,NC 27709. RP ZHANG, J (reprint author), UNIV N CAROLINA,DEPT EPIDEMIOL,CB 7400,CHAPEL HILL,NC 27599, USA. NR 22 TC 60 Z9 62 U1 1 U2 4 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 207 EP 210 DI 10.2105/AJPH.83.2.207 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300008 PM 8427324 ER PT J AU SIMONSMORTON, BG TAYLOR, WC SNIDER, SA HUANG, IW AF SIMONSMORTON, BG TAYLOR, WC SNIDER, SA HUANG, IW TI THE PHYSICAL-ACTIVITY OF 5TH-GRADE STUDENTS DURING PHYSICAL-EDUCATION CLASSES SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Note ID HEALTHFUL DIET; PUBLIC-HEALTH; PARTICIPATION; CHILDHOOD; CHILDREN; FITNESS AB One hundred fifty-seven fifth-grade students in 20 of the 355 elementary schools in one Texas county were systematically observed during physical education classes. On average, the students spent 8.5% of class time in moderate to vigorous physical activity, 23.3% in minimal activity, and 68.1% in sedentary activity. None of the schools averaged 20% of class time in moderate to vigorous physical activity. The levels of physical activity observed are substantially lower than the levels called for in national health objectives. C1 UNIV TEXAS,HLTH SCI CTR,CTR HLTH PROMOT RES & DEV,HOUSTON,TX 77225. RP SIMONSMORTON, BG (reprint author), NICHHD,DIV EPIDEMIOL STAT & PREVENT RES,6100 EXECUT BLVD N,ROOM 7B05,BETHESDA,MD 20892, USA. NR 15 TC 55 Z9 55 U1 1 U2 2 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 262 EP 264 DI 10.2105/AJPH.83.2.262 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300021 PM 8427337 ER PT J AU SALIVE, ME GURALNIK, JM GLYNN, RJ AF SALIVE, ME GURALNIK, JM GLYNN, RJ TI LEFT-HANDEDNESS AND MORTALITY SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Note ID LIFE-SPAN AB We examined mortality associated with handedness in two ways. A simulation using national data suggests that lower mean age at death among left-handed persons, previously offered as evidence of higher mortality, can be explained exclusively by the age distribution of laterality. Second, empiric evidence from a 6-year cohort study of 3774 older adults from East Boston, Massachusetts, demonstrates that left-handedness is not associated with mortality (relative odds = 1.04, confidence inter-val = 0.79, 1.36). C1 HARVARD UNIV,SCH MED,CHANNING LAB,DEPT MED,BOSTON,MA 02115. RP SALIVE, ME (reprint author), NIA,EPIDEMIOL DEMOG & BIOMETRY PROGRAM,7201 WISCONSIN AVE,GATEWAY BLDG,BETHESDA,MD 20892, USA. NR 14 TC 21 Z9 21 U1 1 U2 2 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 265 EP 267 DI 10.2105/AJPH.83.2.265 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300022 PM 8427338 ER PT J AU STEEL, E FLEMING, PL NEEDLE, R AF STEEL, E FLEMING, PL NEEDLE, R TI THE HIV RATES OF INJECTION-DRUG USERS IN LESS-POPULATED AREAS SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Letter C1 NATL CTR INFECT DIS,ATLANTA,GA. RP STEEL, E (reprint author), NIDA,5600 FISHERS LANE,ROOM 10A38,PARKLAWN BLDG,ROCKVILLE,MD 20857, USA. NR 5 TC 4 Z9 4 U1 0 U2 0 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD FEB PY 1993 VL 83 IS 2 BP 286 EP 287 DI 10.2105/AJPH.83.2.286-a PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA KW273 UT WOS:A1993KW27300029 PM 8427345 ER PT J AU OKAYAMA, M MULLOL, J BARANIUK, JN HAUSFELD, JN FELDMAN, B MERIDA, M SHELHAMER, JH KALINER, MA AF OKAYAMA, M MULLOL, J BARANIUK, JN HAUSFELD, JN FELDMAN, B MERIDA, M SHELHAMER, JH KALINER, MA TI MUSCARINIC RECEPTOR SUBTYPES IN HUMAN NASAL-MUCOSA - CHARACTERIZATION, AUTORADIOGRAPHIC LOCALIZATION, AND FUNCTION-INVITRO SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID HUMAN PERIPHERAL LUNG; SMOOTH-MUSCLE; CHOLINERGIC RECEPTORS; NERVE-STIMULATION; THORACIC AORTA; BLOOD-VESSELS; GUINEA-PIG; CAT; ANTAGONISTS; BINDING AB Muscarinic receptors play important roles in the regulation of glandular secretion and vasomotor tone in human nasal mucosa. M1, M2, and M3 muscarinic receptor subtypes were pharmacologically characterized in human inferior turbinates by receptor-binding assays using [H-3](-)quinuclidinyl benzilate (QNB, identifies total muscarinic receptors) and [H-3]-pirenzepine (PZ). Receptors were localized by autoradiography, and their function examined in vitro by assaying mucus secretion from cultured nasal mucosal explants. In competition assays, PZ was employed as a selective muscarinic antagonist for M1 receptors, gallamine and AF-DX 116 for M2 receptors, and 4-DAMP for M3 receptors. These ligands are selective at low nanomolar concentrations, but can interact with other muscarinic receptors at higher concentrations. It is not known if they can interact with putative M4 and M5 muscarinic receptor subtypes. Using [H-3](-)QNB, total muscarinic receptor binding was 688.4 +/- 49.6 fmol/mg protein (B(max)), with a K(d) of 1.47 +/- 0.13 nM. [H-3]-PZ bound to 45 % of the total QNB binding sites. In competition experiments, 4-DAMP displaced [H-3](-)QNB with the lowest IC50, followed by PZ and AF-DX 116. Autoradiograms demonstrated that [H-3](-)QNB binding was completely displaced by 4-DAMP, partially displaced by PZ, but not displaced by gallamine or AF-DX 116, and suggested that M1 and M3 subtypes coexist in submucosal glands. The localization of M1 receptors on submucosal glands was confirmed by direct labeling with [H-3]-PZ. [H-3]-PZ also labeled vessels, but with a low silver grain density. Autoradiographic [H-3]-QNB binding was displaced by 4-DAMP and atropine, but not by PZ, gallamine, or AF-DX 116. In studies of mucus secretion in vitro, 4-DAMP significantly inhibited methacholine-induced secretion. Although less effective, PZ also had significant inhibitory effects. Neither gallamine nor AF-DX 116 had any inhibitory effect. M1 receptors (PZ binding sites) may regulate glandular secretion while M3 receptors (4-DAMP binding sites) may regulate glandular secretion and vasomotor tone in human nasal mucosa. C1 TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 1,SENDAI,MIYAGI 980,JAPAN. WASHINGTON HOSP CTR,DEPT OTOLARYNGOL,WASHINGTON,DC 20010. GEORGETOWN UNIV,DIV RHEUMATOL IMMUNOL & ALLERGY,WASHINGTON,DC 20057. GEORGETOWN UNIV HOSP,DEPT OTOLARYNGOL,WASHINGTON,DC 20007. GEORGETOWN UNIV HOSP,DEPT PEDIAT,WASHINGTON,DC 20007. NIH,CTR CLIN,DEPT MED,DIV CRIT CARE,BETHESDA,MD 20892. NR 56 TC 26 Z9 28 U1 0 U2 1 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD FEB PY 1993 VL 8 IS 2 BP 176 EP 187 PG 12 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA KX408 UT WOS:A1993KX40800009 PM 8427708 ER PT J AU VANDERMEEREN, A SEDDON, MB BETSHOLTZ, CA LECHNER, JF GERWIN, BI AF VANDERMEEREN, A SEDDON, MB BETSHOLTZ, CA LECHNER, JF GERWIN, BI TI TUMORIGENIC CONVERSION OF HUMAN MESOTHELIAL CELLS AS A CONSEQUENCE OF PLATELET-DERIVED GROWTH FACTOR-A CHAIN OVEREXPRESSION SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID SIMIAN SARCOMA-VIRUS; C-SIS GENE; PDGF-A; V-SIS; B-CHAIN; CHROMOSOMAL-ABNORMALITIES; MALIGNANT MESOTHELIOMA; TRANSFORMED-CELLS; HUMAN-FIBROBLASTS; SV40-TRANSFORMED CELLS AB Overexpression of platelet-derived growth factor (PDGF)-A as well as PDGF-B chain mRNA has previously been reported in human mesothelioma cell lines. In this report, it has been established that the A but not the B chain protein is expressed at detectable levels in cell lysates and conditioned medium from these cell lines. In order to investigate the effect of overexpression of PDGF-A chain in a human mesothelial cell model system, a retroviral vector containing a human PDGF-A chain cDNA insert under the control of the Moloney murine leukemia virus (MoMLV) promoter was inserted into the SV-40 T-antigen immortalized human mesothelial cell line MeT-5A. Selected cells showed overexpression of PDGF-A chain relative to MeT-5A and induced tumors in athymic nude mice. PDGF-A chain overexpression was also found in the tumor specimens excised from the mice. PDGF-A mRNA and protein were expressed at a higher level in the tumor explant cell lines, suggesting a correlation of tumorigenicity with A chain production. C1 NCI,HUMAN CARCINOGENESIS LAB,BLDG 37,ROOM 2C08,BETHESDA,MD 20892. UNIV HOSP UPPSALA,DEPT PATHOL,UPPSALA,SWEDEN. INHALAT TOXICOL RES INST,ALBUQUERQUE,NM. NR 69 TC 25 Z9 25 U1 1 U2 1 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD FEB PY 1993 VL 8 IS 2 BP 214 EP 221 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA KX408 UT WOS:A1993KX40800014 PM 8427711 ER PT J AU ZARATEOSORNO, A MEDEIROS, LJ KINGMA, DW LONGO, DL JAFFE, ES AF ZARATEOSORNO, A MEDEIROS, LJ KINGMA, DW LONGO, DL JAFFE, ES TI HODGKINS-DISEASE FOLLOWING NON-HODGKINS-LYMPHOMA - A CLINICOPATHOLOGICAL AND IMMUNOPHENOTYPIC STUDY OF 9 CASES SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE HODGKINS DISEASE; NON-HODGKINS LYMPHOMA; IMMUNOHISTOCHEMISTRY ID MYCOSIS-FUNGOIDES; COMPOSITE LYMPHOMA; HETEROGENEITY; MALIGNANCIES; INVOLVEMENT; ANTIBODIES; LEUKEMIA; PATIENT; ORIGIN; CELL AB We describe nine patients who initially developed non-Hodgkin's lymphoma and subsequently developed Hodgkin's disease. The median interval from the diagnosis of non-Hodgkin's lymphoma (NHL) to the diagnosis of Hodgkin's disease (HD) was 5 years (range, 2-12 years). The median age of the patients at time of diagnosis of NHL was 54 years (range, 27-81 years). Seven of nine cases (78%) of NHL were primarily nodal. According to the Working Formulation, seven NHL were follicular (two small cleaved cell, three mixed small and large cell, two large cell), one was diffuse large cell, and one was large cell immunoblastic. All NHL had histologic or immunophenotypic findings indicative of B-cell lineage. Seven of the nine patients were treated in a nonuniform manner: four with chemotherapy and three with chemotherapy and radiation therapy. At the time of HD, the median age of the patients was 59 years (range, 35-85 years). Lymph nodes were involved in all patients. Six HD biopsies were subclassified as nodular sclerosis, one as mixed cellularity, and two cases were not further subclassified. Immunophenotypic studies revealed that the Reed-Sternberg and Hodgkin cells were LeuM1 or BerH2 positive and LCA negative in eight of nine biopsies, supporting the histologic diagnosis. These results further demonstrate that patients with NHL may subsequently develop HD. The NHLs are usually of B-cell lineage. The results also emphasize the need for rebiopsy in patients with NHL who experience an apparent clinical relapse. C1 NCI,DIV CANC BIOL DIAGNOSIS & CTR,PATHOL LAB,HEMATOPATHOL SECT,BETHESDA,MD 20892. NCI,BIOL RESPONSE MODIFIERS PROGRAM,BETHESDA,MD 20892. NR 32 TC 27 Z9 27 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD FEB PY 1993 VL 17 IS 2 BP 123 EP 132 DI 10.1097/00000478-199302000-00004 PG 10 WC Pathology; Surgery SC Pathology; Surgery GA KJ461 UT WOS:A1993KJ46100004 PM 8422110 ER PT J AU KURMAN, RJ TOKI, T SCHIFFMAN, MH AF KURMAN, RJ TOKI, T SCHIFFMAN, MH TI BASALOID AND WARTY CARCINOMAS OF THE VULVA - DISTINCTIVE TYPES OF SQUAMOUS-CELL CARCINOMA FREQUENTLY ASSOCIATED WITH HUMAN PAPILLOMAVIRUSES SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE VULVA; HUMAN PAPILLOMAVIRUS; WARTY CARCINOMA; BASALOID CARCINOMA; KERATINIZING SQUAMOUS CELL CARCINOMA ID POLYMERASE CHAIN-REACTION; INSITU HYBRIDIZATION; INTRAEPITHELIAL NEOPLASIA; PROGNOSTIC FACTORS; ETIOLOGY; SURVIVAL AB In a previous study, we described an elevated prevalence of human papillomavirus (HPV) in two specific types of squamous cell carcinoma of the vulva designated basaloid carcinoma (BC) and warty carcinoma (WC) compared with the conventional type of keratinizing squamous cell carcinoma (KSC). To determine whether there were other differences in their clinical presentation or behavior, we examined 100 cases of squamous cell carcinoma of the vulva classified as BC (28 cases), WC (seven cases), and KSC (65 cases). We included only cases in which tissue adjacent to the tumor was present so that the presence of intraepithelial lesions (squamous hyperplasia, lichen sclerosus, and vulvar intraepithelial neoplasia [VIN]) could be correlated with the different types of invasive carcinomas. Microscopically, BC was characterized by a relatively uniform population of small, ovoid cells with a high nuclear-cytoplasmic ratio resembling VIN 3. Although WC was similar to typical squamous cell carcinoma, it contained many squamous cells that displayed marked nuclear pleomorphism, enlargement, atypia, and multinucleation in conjunction with cytoplasmic cavitation resembling koilocytotic atypia in intraepithelial lesions. The majority of the women with BC and WC were less than 60 years of age, and the proportion of black women was higher as compared with the women with KSC, the majority of whom were white and over 65 years of age. On crude comparison, women with BC appeared to have a survival advantage compared with women with KSC; however, through multivariate modelling, when all possible confounding variables were taken into account, there was little residual impression of a survival advantage of women with BC compared with those having KSC. Substantial differences were found among the three types of carcinoma with regard to the prevalence of adjacent intraepithelial lesions. Squamous hyperplasia was found adjacent to KSC in 54 (83%) of the 65 cases, whereas 27 (77%) of 35 cases of BC and WC had adjacent basaloid or warty VIN. These findings suggest that VIN is a precursor of BC and WC. In view of the high frequency of HPV-DNA detected in VIN and in BC and WC, the findings support the view that HPV has a role in the development of these tumors. In addition, a difference was found in the distribution of associated cervical and vaginal tumors with the three types of vulvar carcinomas. Seven (23%) of the 30 women with BC or WC for whom data were available had another invasive or noninvasive squamous neoplasm of the cervix or vagina, compared with only three (5%) of women with KSC, suggesting that BC and WC arise as a result of a ''field effect.'' Based on this preliminary analysis and other studies, we conclude that squamous cell carcinomas of the vulva are etiologically diverse tumors. of which a small but substantial proportion (BC and WC) are related to HPV, whereas the majority (KSC) are not. Future studies should distinguish these tumors to facilitate identification of other epidemiologic risk factors and to clarify their histogenesis. C1 NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,DEPT OBSTET & GYNECOL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,BALTIMORE,MD 21205. RP KURMAN, RJ (reprint author), JOHNS HOPKINS UNIV HOSP,DEPT PATHOL,ROOM 711,600 N WOLFE ST,BALTIMORE,MD 21205, USA. NR 20 TC 165 Z9 167 U1 0 U2 3 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD FEB PY 1993 VL 17 IS 2 BP 133 EP 145 DI 10.1097/00000478-199302000-00005 PG 13 WC Pathology; Surgery SC Pathology; Surgery GA KJ461 UT WOS:A1993KJ46100005 PM 8380681 ER PT J AU MONTERROSO, V JAFFE, ES MERINO, MJ MEDEIROS, LJ AF MONTERROSO, V JAFFE, ES MERINO, MJ MEDEIROS, LJ TI MALIGNANT-LYMPHOMAS INVOLVING THE OVARY - A CLINICOPATHOLOGICAL ANALYSIS OF 39 CASES SO AMERICAN JOURNAL OF SURGICAL PATHOLOGY LA English DT Article DE NON-HODGKINS LYMPHOMA; OVARY; B-CELL; IMMUNOPHENOTYPE ID FEMALE GENITAL-TRACT; HODGKINS-DISEASE; PRIMARY MANIFESTATION; BURKITTS-LYMPHOMA; CELL LYMPHOMA; LYMPHOCYTES; INVOLVEMENT; ANDROGEN; ESTROGEN; FEATURES AB We report 39 patients with non-Hodgkin's lymphoma who initially presented with ovarian enlargement. Fifteen patients had unilateral (10 left, four right, one unknown) and 24 had bilateral ovarian masses. The median size of the tumors was 8 cm (range, 2 to 23 cm). Histologically, using the Working Formulation, 21 lesions (54%) were classified as small noncleaved cell, Burkitt's type; 12 (31%) as large cell (nine diffuse, three focally follicular): three (8%) as diffuse mixed, small and large cell; two (5%) as large cell immunoblastic; and one (2%) as follicular and diffuse small cleaved cell. Twenty-six tumors were analyzed immunophenotypically, 25 (96%) of which were B-cell neoplasms. However, combining histologic and immunohistochemical findings, 37 neoplasms were of B-cell lineage, one diffuse large-cell lymphoma was not analyzed, and one large-cell immunoblastic lymphoma (with features of anaplastic large-cell lymphoma) was of T-cell lineage with an aberrant immunophenotype. On the basis of staging studies and clinical follow-up, we conclude that only four (10%) of the neoplasms in this study most likely arose in the ovary. The primary neoplasms, three diffuse large-cell and one diffuse mixed small- and large-cell, were B-cell neoplasms. Three of four patients with primary neoplasms were apparently cured at last follow-up following surgical excision and chemotherapy. The remainder of the lymphomas in this study, most commonly of small noncleaved cell. Burkitt's type, appear to be systemic tumors that involved the ovaries secondarily. Approximately 40% of patients with systemic neoplasms were alive without evidence of disease at last follow-up. C1 NCI,PATHOL LAB,BLDG 10,ROOM 2N212,BETHESDA,MD 20892. NR 39 TC 91 Z9 97 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0147-5185 J9 AM J SURG PATHOL JI Am. J. Surg. Pathol. PD FEB PY 1993 VL 17 IS 2 BP 154 EP 170 DI 10.1097/00000478-199302000-00007 PG 17 WC Pathology; Surgery SC Pathology; Surgery GA KJ461 UT WOS:A1993KJ46100007 PM 8422111 ER PT J AU HEEGAARD, NHH AF HEEGAARD, NHH TI INTERACTIONS OF IGG WITH SPECIFIC ERYTHROCYTE-MEMBRANE PROTEINS IN AFFINITY ELECTROPHORESIS ARE HIGHLY DEPENDENT ON LOW IONIC-STRENGTH CONDITIONS SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID BAND-3; ANKYRIN; DOMAIN C1 ST ELIZABETH HOSP, NIMH, CTR NEUROSCI, BIOCHEM GENET LAB, WASHINGTON, DC 20032 USA. NR 17 TC 5 Z9 5 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 EI 1096-0309 J9 ANAL BIOCHEM JI Anal. Biochem. PD FEB 1 PY 1993 VL 208 IS 2 BP 317 EP 322 DI 10.1006/abio.1993.1053 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA KK036 UT WOS:A1993KK03600019 PM 8452227 ER PT J AU GRIMALDI, CH NELSON, RG PETTITT, DJ SAMPLINER, RE BENNETT, PH KNOWLER, WC AF GRIMALDI, CH NELSON, RG PETTITT, DJ SAMPLINER, RE BENNETT, PH KNOWLER, WC TI INCREASED MORTALITY WITH GALLSTONE DISEASE - RESULTS OF A 20-YEAR POPULATION-BASED SURVEY IN PIMA-INDIANS SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE CHOLELITHIASIS; INDIANS, NORTH AMERICAN; MORTALITY; ARIZONA; GALLBLADDER NEOPLASMS ID CORONARY HEART-DISEASE; GALLBLADDER CANCER; HIGH PREVALENCE; COLON CANCER; BILE-ACIDS; DIABETES-MELLITUS; NATURAL-HISTORY; RISK-FACTORS; LARGE BOWEL; CHOLECYSTECTOMY AB Objective: To determine if gallstone disease is associated with an increased risk for malignancy and higher total mortality in Pima Indians. Design: Inception cohort. Setting: American Indian community. Participants: Age- and sex-stratified random population-based sample. Measurements: Between 1966 and 1969, an age- and sex-stratified random sample of Pima Indians from the Gila River Indian Community in Arizona was examined to identify evidence of gallstone disease defined as either gallstones (oral cholecystography) or previous cholecystectomy. During 20 years of follow-up, deaths were recorded and underlying causes of death, according to death certificates, were determined. Results: Among 383 persons with known gallbladder status, 186 (49%) died: 133 among the 222 persons with gallstone disease and 53 among the 161 without. The overall death rate was higher in persons with gallstone disease than in those with normal gallbladders. The age- and sex-adjusted death rate ratio was 1.9 (95% CI, 1.3 to 2.7). Furthermore, the death rate attributed to malignancies was 6.6 times (CI, 1.3 to 33.1) as high in persons with gallstone disease as in those with normal gallbladders. Of the 20 fatal malignancies in persons with gallstone disease, 11 occurred in the digestive tract, of which six involved the gallbladder or bile ducts. Conclusions: Increased cancer mortality and total mortality were found in Pima Indians with gallstone disease. Although plausible explanations exist for the increased cancer mortality, the increased death rates due to other causes are unexplained. Whether cholecystectomy would change this risk is unknown. C1 NIDDKD,PHOENIX EPIDEMIOL & CLIN RES BRANCH,PHOENIX,AZ. CLEVELAND CLIN EDUC FDN,CLEVELAND,OH 44106. VET AFFAIRS MED CTR,TUCSON,AZ. UNIV ARIZONA,TUCSON,AZ 85721. RP GRIMALDI, CH (reprint author), HOP CIMIEZ,4 AV REINE VICTORIA,F-06000 NICE,FRANCE. RI Nelson, Robert/B-1470-2012 NR 55 TC 17 Z9 17 U1 0 U2 3 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD FEB 1 PY 1993 VL 118 IS 3 BP 185 EP 190 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA KJ439 UT WOS:A1993KJ43900005 PM 8417635 ER PT J AU KALER, SG GOLDSTEIN, DS HOLMES, C SALERNO, JA GAHL, WA AF KALER, SG GOLDSTEIN, DS HOLMES, C SALERNO, JA GAHL, WA TI PLASMA AND CEREBROSPINAL-FLUID NEUROCHEMICAL PATTERN IN MENKES DISEASE SO ANNALS OF NEUROLOGY LA English DT Article ID BETA-HYDROXYLASE DEFICIENCY; BRINDLED MOUSE; NOREPINEPHRINE; HUMANS; 3,4-DIHYDROXYPHENYLALANINE; DIHYDROXYPHENYLGLYCOL; NORADRENALINE; DOPA AB Menkes disease is a neurodegenerative disorder of copper metabolism. Because the enzyme dopamine-beta-hydroxylase requires copper to catalyze the conversion of dopamine to norepinephrine, we reasoned that patients with Menkes disease would have a neurochemical pattern similar to that seen in patients with congenital absence of dopamine-beta-hydroxylase, i.e., high levels of dopamine, the dopamine metabolite dihydroxyphenylacetic acid (DOPAC), and the catecholamine precursor dihydroxyphenylalanine (DOPA), and low levels of norepinephrine and its neuronal metabolite dihydroxyphenylglycol (DHPG). We measured plasma and cerebrospinal fluid (CSF) levels of catechols in 10 patients ranging in age from 9 days to 27 months. In contrast to patients with congenital absence of dopamine-beta-hydroxylase, norepinephrine levels were normal in plasma of 4 Menkes patients and in CSF of all 10 patients. However, the ratios of DOPA: DHPG and DOPAC: DHPG in plasma and CSF of Menkes patients were invariably increased beyond the ranges of control values. These neurochemical findings indicate partial deficiency of dopamine-beta-hydroxylase in Menkes patients, with compensatory increases in catecholamine biosynthesis in sympathetic nerves and in the brain. Increased tyrosine hydroxylation and increased exocytotic release of norepinephrine may be responsible for preservation of plasma and CSF norepinephrine levels in Menkes patients. The abnormal neurochemical pattern, including high ratios of DOPA: DHPG and DOPAC: DHPG, may serve as a biochemical marker for Menkes disease and provide a baseline against which the influence of proposed therapies can be judged. C1 NINCDS,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. NIA,NEUROSCI LAB,BETHESDA,MD 20892. RP KALER, SG (reprint author), NICHHD,HUMAN GENET BRANCH,BLDG 10,ROOM 9S 242,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 28 TC 63 Z9 63 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0364-5134 J9 ANN NEUROL JI Ann. Neurol. PD FEB PY 1993 VL 33 IS 2 BP 171 EP 175 DI 10.1002/ana.410330206 PG 5 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA KX547 UT WOS:A1993KX54700005 PM 8434878 ER PT J AU NICOLETTI, MI LUCCHINI, V MASSAZZA, G ABBOTT, BJ DINCALCI, M GIAVAZZI, R AF NICOLETTI, MI LUCCHINI, V MASSAZZA, G ABBOTT, BJ DINCALCI, M GIAVAZZI, R TI ANTITUMOR-ACTIVITY OF TAXOL (NSC-125973) IN HUMAN OVARIAN CARCINOMAS GROWING IN THE PERITONEAL-CAVITY OF NUDE-MICE SO ANNALS OF ONCOLOGY LA English DT Article DE HUMAN OVARIAN CARCINOMA; NUDE MICE; TAXOL ID PHASE-I; CANCER; MELANOMA; ASCITES; AGENT; TRIAL AB Background: The unique mechanism of action of taxol (NSC-125973) as microtubule stabilizing agent and its potential activity in clinical trials have generated considerable interest in the development of this agent. As taxol was reported to be active on advanced and refractory ovarian carcinomas we focused our studies on the xenograft model of human ovarian carcinoma that develops ascites and tumor dissemination in the peritoneal cavity of nude mice. Methods: The antitumor activity of taxol was evaluated on two human ovarian carcinoma xenografts (HOC8 and HOC22) transplanted i.p. in nude mice. Drug was given i.v. at doses of 20-34.5 mg/kg every four days three times (Q4 x 3) and the increment of life span (%ILS) was evaluated. Cisplatin at the dosage of 4mg/kg, Q4 x 3 was used as reference drug in each experiment. Results: Taxol given at doses of 20 mg/kg and 34.5 mg/kg to early-stage HOC22 (treatment starting 3 days after tumor transplant) cured all the mice, while the same dose regimens given to advanced HOC22 (treatment starting 14 days after tumor transplant) significantly prolonged the survival time of the mice (ILS = 197% and 300%). Taxol given 3 days after HOC8 transplant significantly prolonged the survival time of tumor-bearing nude mice, inducing complete responses in 50% and 25% of mice receiving, respectively, 34.5 mg/kg/injection and 20 mg/kg/injection. On both ovarian carcinoma xenografts taxol was more active than equitoxic doses of the reference drug cisplatin. Conclusions: The therapeutic activity against ovarian carcinoma xenografts supports the potential of taxol in the treatment of this neoplasia and forms the basis for future investigations aimed at optimizing the therapeutic activity of taxol given alone or in combination with other drugs. C1 IST RIC FARMACOL MARIO NEGRI,VIA GAVAZZENI,BERGAMO,ITALY. SAN GERARDO HOSP,DEPT PATHOL,MONZA,ITALY. MARIO NEGRI INST PHARMACOL RES,I-20157 MILAN,ITALY. NCI,DEV THERAPEUT PROGRAM,FREDERICK,MD 21701. NR 19 TC 28 Z9 28 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0923-7534 J9 ANN ONCOL JI Ann. Oncol. PD FEB PY 1993 VL 4 IS 2 BP 151 EP 155 PG 5 WC Oncology SC Oncology GA KQ281 UT WOS:A1993KQ28100012 PM 8095399 ER PT J AU BROWN, PS ROBERTS, CS MCINTOSH, CL SWAIN, JA CLARK, RE AF BROWN, PS ROBERTS, CS MCINTOSH, CL SWAIN, JA CLARK, RE TI LATE RESULTS AFTER TRIPLE-VALVE REPLACEMENT WITH VARIOUS SUBSTITUTE VALVES SO ANNALS OF THORACIC SURGERY LA English DT Article ID FOLLOW-UP; PERIOPERATIVE MORTALITY; TRICUSPID REGURGITATION; NONOPERATIVE MANAGEMENT; ANNULOPLASTY AB The purpose of this study was to determine what influence various combinations of mechanical and bioprosthetic valves in the aortic, mitral, and tricuspid positions had on late morbidity and mortality of 40 hospital survivors of triple-valve replacement. At operation the patients ranged in age from 27 to 69 years; 73% were women. The mean postoperative follow-up interval was 8.3 years, with a total follow-up of 331 years (100% complete). At 12 months after operation, functional class decreased from 3.3 to 1.6 (p < 0.05), cardiac index increased from 2.0 to 2.6 L . min-1 . m-2 (p < 0.05), and pulmonary artery pressures decreased from 59/27 to 40/17 mm Hg (p < 0.05). There were no differences in preoperative variables between groups. Actuarial survival for the 40 patients (exclusive of 30-day or in-hospital mortality, which was 31%) was 78% and 74% at 5 and 10 years. At the same milestones, freedom from reoperation was 96% and 54%, freedom from combined thromboembolism and anticoagulant-related hemorrhage was 68% and 56%, and freedom from all late valve-related morbidity and mortality was 64% and 25%. Comparison of the patients with two or more mechanical prostheses with the patients having two or more bioprostheses indicated no significant differences in actuarial freedom from late death, thromboembolic events, or anticoagulant-related hemorrhage. However the actuarial freedom from reoperation in the groups with two or more mechanical valves was lower than that of the groups with two or more bioprosthetic valves (0/10 versus 13/30; p < 0.05). Among 13 patients having reoperation, reoperation in 12 was prompted by degeneration of one or more bioprosthetic valves. Six of the patients who underwent reoperation died in the hospital, and 4 others died between 2 and 8 years after operation. These results support the view that mechanical prostheses provide better long-term results for triple-valve replacement than those produced using bioprostheses, primarily by reducing need for reoperation and its attendant complications. C1 NHLBI,SURG BRANCH,BETHESDA,MD 20892. NR 24 TC 15 Z9 15 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD FEB PY 1993 VL 55 IS 2 BP 502 EP 508 PG 7 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA KK914 UT WOS:A1993KK91400034 PM 8431067 ER PT J AU REUVENY, S KIM, YJ KEMP, CW SHILOACH, J AF REUVENY, S KIM, YJ KEMP, CW SHILOACH, J TI EFFECT OF TEMPERATURE AND OXYGEN ON CELL-GROWTH AND RECOMBINANT PROTEIN-PRODUCTION IN INSECT CELL-CULTURES SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article ID NUCLEAR POLYHEDROSIS-VIRUS AB The effect of temperature and 02 saturation on the production of recombinant proteins beta-galactosidase and human glucocerebrosidase by Spodoptera frugiperda cells (Sf9) infected with recombinant Autographa californica nuclear polyhedrosis virus was investigated. The rates of cell growth, glucose consumption, O2 consumption and product expression were measured at temperatures between 22-degrees-C and 35-degrees-C. The results indicated that possible O2 limitation may be alleviated without compromising the maximum cell yield by lowering the incubation temperature from 27-degrees-C to 25-degrees-C. The expression level of the recombinant proteins at 27-degrees-C was similar to that obtained at 22-degrees-C and 25-degrees-C; lower protein yields were obtained at 30-degrees-C. An increase in temperature from 22-degrees-C to 27-degrees-C led to earlier production of the proteins and to an increase in the proportion of the product released outside the cells. C1 NIDDK,LCDB,BIOTECHNOL UNIT,BLDG 6,ROOM B1-33,BETHESDA,MD 20892. BIOWHITTAKER INC,WALKERSVILLE,MD 21793. NR 13 TC 61 Z9 61 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0175-7598 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD FEB PY 1993 VL 38 IS 5 BP 619 EP 623 PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA KP353 UT WOS:A1993KP35300009 PM 7763472 ER PT J AU KNUTTEL, A SCHMITT, JM KNUTSON, JR AF KNUTTEL, A SCHMITT, JM KNUTSON, JR TI SPATIAL LOCALIZATION OF ABSORBING BODIES BY INTERFERING DIFFUSIVE PHOTON-DENSITY WAVES SO APPLIED OPTICS LA English DT Article DE SPATIAL LOCALIZATION; INTERFERENCE; PHOTON DIFFUSION; REFLECTANCE MODE ID TIME; TISSUE; MIGRATION; MODEL AB The use of the destructive interference of diffusive photon-density waves for the localization of an absorbing (and scattering) body in a scattering medium was studied. The objectives of this approach in the reflectance mode were as follows: first, to reduce sensitivity to absorption features occurring in superficial layers while sensitivity to bodies lying deeper is maintained; second, to establish a confined depth region of maximum sensitivity in which the distance of an absorbing body could be determined through phase measurement. Intensity and phase data were acquired with a modified frequency-domain spectrometer at modulation frequencies up to 600 MHz as an absorbing body was moved in three dimensions. The experimental results are compared with simulations based on a numerical solution of a time-dependent photon-diffusion equation. C1 NIH, BIOMED ENGN & INSTRUMENTAT PROGRAM, BETHESDA, MD 20892 USA. NIH, CELL BIOL LAB, BETHESDA, MD 20892 USA. RP KNUTTEL, A (reprint author), NHLBI, CARDIAC ENERGET LAB, BETHESDA, MD 20892 USA. NR 28 TC 69 Z9 71 U1 0 U2 2 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 0003-6935 J9 APPL OPTICS JI Appl. Optics PD FEB 1 PY 1993 VL 32 IS 4 BP 381 EP 389 PG 9 WC Optics SC Optics GA KJ631 UT WOS:A1993KJ63100003 PM 20802701 ER PT J AU GANDJBAKHCHE, AH NOSSAL, R BONNER, RF AF GANDJBAKHCHE, AH NOSSAL, R BONNER, RF TI SCALING RELATIONSHIPS FOR THEORIES OF ANISOTROPIC RANDOM-WALKS APPLIED TO TISSUE OPTICS SO APPLIED OPTICS LA English DT Article ID DIFFUSING-WAVE SPECTROSCOPY; SIMILARITY RELATIONS; RANDOM-MEDIA; APPROXIMATION; SCATTERING; TRANSPORT AB Monte Carlo simulations are used to discern scaling relationships for photon migration occurring within homogeneous, anisotropic scattering media of semi-infinite extent. Special attention is given to events associated with short path lengths. Empirical scaling relationships for path lengths and surface intensities are shown to agree with a consistency equation derived in an earlier study of anisotropic random walks. They are augmented here by a procedure that accounts for concomitant scaling of optical absorption coefficients. Results then are used to transform expressions that were obtained previously by analytical random-walk theory developed for an isotropic scattering model of photon migration. Quantities that are studied include the diffuse surface reflectance, the depth distribution of the fluence, and the time-resolved intensity of backreflected photons. RP GANDJBAKHCHE, AH (reprint author), NIH, DEPT HLTH & HUMAN SERV, BETHESDA, MD 20892 USA. RI Bonner, Robert/C-6783-2015 NR 17 TC 60 Z9 61 U1 0 U2 4 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 0003-6935 J9 APPL OPTICS JI Appl. Optics PD FEB 1 PY 1993 VL 32 IS 4 BP 504 EP 516 PG 13 WC Optics SC Optics GA KJ631 UT WOS:A1993KJ63100020 PM 20802718 ER PT J AU HAVLIN, S KIEFER, JE TRUS, B WEISS, GH NOSSAL, R AF HAVLIN, S KIEFER, JE TRUS, B WEISS, GH NOSSAL, R TI NUMERICAL-METHOD FOR STUDYING THE DETECTABILITY OF INCLUSIONS HIDDEN IN OPTICALLY TURBID TISSUE SO APPLIED OPTICS LA English DT Article ID TRANSILLUMINATION IMAGING PERFORMANCE; PHOTON MIGRATION; BREAST TISSUES; TRANS-ILLUMINATION; TIME; SCATTERING; MEDIA; COEFFICIENTS; DIFFUSION; SYSTEM AB We introduce an efficient numerical method for studying the detectability of absorptive inclusions in a multiple-scattering optical medium. Use of the method is demonstrated by the forward calculation of integrated and time-gated photon intensities. Schemes for positioning the light source above an inclusion and otherwise determining the location of a hidden object, involving either reflected or transmitted reemissions, are discussed as examples. They are investigated for several illustrative models, and images are calculated as a function of the size and shape of the inclusion. RP HAVLIN, S (reprint author), NIH, DIV COMP RES & TECHNOL, BETHESDA, MD 20892 USA. NR 42 TC 12 Z9 12 U1 0 U2 0 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 0003-6935 J9 APPL OPTICS JI Appl. Optics PD FEB 1 PY 1993 VL 32 IS 4 BP 617 EP 627 PG 11 WC Optics SC Optics GA KJ631 UT WOS:A1993KJ63100035 ER PT J AU REGIER, DA NARROW, WE RAE, DS MANDERSCHEID, RW LOCKE, BZ GOODWIN, FK AF REGIER, DA NARROW, WE RAE, DS MANDERSCHEID, RW LOCKE, BZ GOODWIN, FK TI THE DE-FACTO-UNITED-STATES-MENTAL-AND-ADDICTIVE-DISORDERS-SERVICE-SYSTEM SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID CATCHMENT-AREA PROGRAM; HEALTH-SERVICES; DRUG-ABUSE; RELIABILITY; COMMUNITY; CARE; POPULATION; PREVALENCE; DEPRESSION; DIAGNOSES AB After initial interviews with 20 291 adults in the National Institute of Mental Health Epidemiologic Catchment Area Program, we estimated prospective 1-year prevalence and service use rates of mental and addictive disorders in the US population. An annual prevalence rate of 28.1% was found for these disorders, composed of a 1-month point prevalence of 15.7% (at wave 1) and a 1-year incidence of new or recurrent disorders identified in 12.3% of the population at wave 2. During the 1-year follow-up period, 6.6% of the total sample developed one or more new disorders after being assessed as having no previous lifetime diagnosis at wave 1. An additional 5.7% of the population, with a history of some previous disorder at wave 1, had an acute relapse or suffered from a new disorder in 1 year. irrespective of diagnosis, 14.7% of the US population in 1 year reported use of services in one or more component sectors of the de facto US mental and addictive service system. With some overlap between sectors, specialists in mental and addictive disorders provided treatment to 5.9% of the US population, 6.4% sought such services from general medical physicians, 3.0% sought these services from other human service professionals, and 4.1% turned to the voluntary support sector for such care. Of those persons with any disorder, only 28.5% (8.0 per 100 population) sought mental health/addictive services. Persons with specific disorders varied in the proportion who used services, from a high of more than 60% for somatization, schizophrenia, and bipolar disorders to a low of less than 25% for addictive disorders and severe cognitive impairment. Applications of these descriptive data to US health care system reform options are considered in the context of other variables that will determine national health policy. C1 NIMH,DIV EPIDEMIOL & SERV RES,EPIDEMIOL & PSYCOPATHOL RES BRANCH,ROCKVILLE,MD 20857. SUBST ABUSE & MENTAL HLTH SERV ADM,CTR MENTAL HLTH SERV,DIV STATE & COMMUNITY SYST DEV,ROCKVILLE,MD. NR 53 TC 1307 Z9 1322 U1 12 U2 47 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1993 VL 50 IS 2 BP 85 EP 94 PG 10 WC Psychiatry SC Psychiatry GA KL361 UT WOS:A1993KL36100001 PM 8427558 ER PT J AU NARROW, WE REGIER, DA RAE, DS MANDERSCHEID, RW LOCKE, BZ AF NARROW, WE REGIER, DA RAE, DS MANDERSCHEID, RW LOCKE, BZ TI USE OF SERVICES BY PERSONS WITH MENTAL AND ADDICTIVE DISORDERS - FINDINGS FROM THE NATIONAL-INSTITUTE-OF-MENTAL-HEALTH-EPIDEMIOLOGIC-CATCHMENT-AREA-PROGRAM SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID MEXICAN-AMERICANS; MEDICAL-CARE; SOMATIZATION; DEPRESSION; POPULATION; SPECIALTY AB The use of ambulatory and inpatient mental health and addiction services in the United States was estimated by means of data from the National Institute of Mental Health Epidemiologic Catchment Area Program standardized to the 1980 US census for adults 18 years of age and older. In a 1-year period, 22.8 million people used ambulatory services for mental or addictive disorder treatment; 54% of them had a current Diagnostic Interview Schedule/DSM-III mental disorder and another 37.4% had a history of psychiatric disorder or significant psychiatric symptoms. A total of 325.9 million ambulatory visits were made, and the average number of visits per treated person per year was 14.3. There were 1.4 million persons admitted to at least one inpatient mental health or addiction setting during a 1-year period; 80% of them had a current DIS/DSM-III disorder, and the remainder had a history of psychiatric disorder or significant psychiatric symptoms. Results were determined for specific mental and substance use diagnoses and service settings. Among treated persons with any mental or addictive disorder, the majority of visits were to mental and addictive disorders specialty settings (40.5% of total visits) and to support networks composed of friends, relatives, and self-help groups (37.0% of total visits). Although a large number of persons with mental and substance use disorders were seen in the general medical sector for mental health or addiction problems, they were seen less frequently and therefore made fewer visits to this sector (10.9% of total visits). C1 NIMH,DIV EPIDEMIOL & SERV RES,EPIDEMIOL & PSYCHOPATHOL RES BRANCH,BETHESDA,MD 20892. SUBST ABUSE & MENTAL HLTH SERV ADM,CTR MENTAL HLTH SERV,DIV STATE & COMMUNITY SYST DEV,ROCKVILLE,MD. NR 39 TC 301 Z9 302 U1 6 U2 10 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1993 VL 50 IS 2 BP 95 EP 107 PG 13 WC Psychiatry SC Psychiatry GA KL361 UT WOS:A1993KL36100002 PM 8381266 ER PT J AU MANDERSCHEID, RW RAE, DS NARROW, WE LOCKE, BZ REGIER, DA AF MANDERSCHEID, RW RAE, DS NARROW, WE LOCKE, BZ REGIER, DA TI CONGRUENCE OF SERVICE UTILIZATION ESTIMATES FROM THE EPIDEMIOLOGIC CATCHMENT-AREA PROJECT AND OTHER SOURCES SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID MENTAL-HEALTH; UNITED-STATES AB Service utilization estimates for inpatient and ambulatory mental health care from the Epidemiologic Catchment Area Project were compared with similar estimates from other sources, principally the Center for Mental Health Services National Reporting Program. Generally, results showed closer correspondence between estimates of the number of persons who used inpatient care than of similar estimates for ambulatory mental health care. Subtotal estimates for the specialty alcohol/other drug abuse/mental health and health care sectors were more similar than were estimates for individual settings. The specialty sector subtotals showed only a 7% difference in patient counts for inpatient care and 13% for ambulatory care, with an 11% difference in visits for the latter. Generally, a reasonable level of congruence was observed, given pronounced differences in methods, procedures, and instruments. Future directions may be able to close data gaps and improve the quality of the national mental health services database. C1 SUBST ABUSE & MENTAL HLTH SERV ADM,CTR MENTAL HLTH SERV,DIV STATE & COMMUNITY SYST DEV,ROCKVILLE,MD. NIMH,DIV EPIDEMIOL & SERV RES,EPIDEMIOL & PSYCHOPATHOL RES BRANCH,ROCKVILLE,MD 20857. NR 22 TC 37 Z9 37 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1993 VL 50 IS 2 BP 108 EP 114 PG 7 WC Psychiatry SC Psychiatry GA KL361 UT WOS:A1993KL36100003 PM 8427550 ER PT J AU SARTORIUS, N KAELBER, CT COOPER, JE ROPER, MT RAE, DS GULBINAT, W USTUN, TB REGIER, DA AF SARTORIUS, N KAELBER, CT COOPER, JE ROPER, MT RAE, DS GULBINAT, W USTUN, TB REGIER, DA TI PROGRESS TOWARD ACHIEVING A COMMON LANGUAGE IN PSYCHIATRY - RESULTS FROM THE FIELD TRIAL OF THE CLINICAL GUIDELINES ACCOMPANYING THE WHO CLASSIFICATION OF MENTAL AND BEHAVIORAL-DISORDERS IN ICD-10 SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID AGREEMENT; RATERS AB In preparing for the 10th revision of the International Classification of Diseases (ICD-10), the Division of Mental Health of the World Health Organization organized an international field trial to help evaluate draft clinical descriptions and diagnostic guidelines that were produced to facilitate use of the chapter dealing with mental and behavioral disorders. These clinical guidelines were prepared in equivalent versions in most of the world's widely spoken languages. The field trial aimed to obtain data that would help in assessing whether the classification fits the diagnoses made in different countries, whether it is easy to use, and whether psychiatrists after a short period of familiarization with the classification can reach agreement about their diagnoses and classification. The field trial was carried out at 112 clinical centers in 39 countries by 711 clinicians who conducted 15 302 individual assessments. The trial included joint dinical assessments of patients and case history exercises. The results of the joint assessment part of the trial are reported here. Most clinicians reported that the draft document was easy to use and that the classification provided a good fit for the vast majority of the clinical conditions encountered. While interrater reliability was satisfactory for most categories, some (for example, those dealing with personality disorders) were somewhat difficult to use, and reliability of assignment for those was lower. The trial demonstrated that the ICD-10 chapter dealing with mental and behavioral disorders is on the whole suitable for general use. It provided valuable indications about changes needed for subsequent versions and demonstrated the feasibility of large-scale international research on classification and diagnosis in psychiatry. C1 NIMH,DIV CLIN RES,ALCOHOL DRUG ABUSE & MENTAL HLTH ADM,ROOM 10C-105,ROCKVILLE,MD 20857. WHO,DIV MENTAL HLTH,CH-1211 GENEVA 27,SWITZERLAND. NR 24 TC 119 Z9 126 U1 0 U2 4 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD FEB PY 1993 VL 50 IS 2 BP 115 EP 124 PG 10 WC Psychiatry SC Psychiatry GA KL361 UT WOS:A1993KL36100004 PM 8427551 ER PT J AU HYDE, TM ZIEGLER, JC WEINBERGER, DR AF HYDE, TM ZIEGLER, JC WEINBERGER, DR TI PSYCHOPATHOLOGY IN METACHROMATIC LEUKODYSTROPHY - REPLY SO ARCHIVES OF NEUROLOGY LA English DT Letter ID SCHIZOPHRENIA RP HYDE, TM (reprint author), NIMH,INTRAMURAL RES PROGRAM,2700 MARTIN LUTHER KING JR AVE SE,WASHINGTON,DC 20032, USA. NR 6 TC 1 Z9 1 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD FEB PY 1993 VL 50 IS 2 BP 131 EP 131 PG 1 WC Clinical Neurology SC Neurosciences & Neurology GA KL174 UT WOS:A1993KL17400004 ER PT J AU DIVECHA, N RHEE, SG LETCHER, AJ IRVINE, RF AF DIVECHA, N RHEE, SG LETCHER, AJ IRVINE, RF TI PHOSPHOINOSITIDE SIGNALING ENZYMES IN RAT-LIVER NUCLEI - PHOSPHOINOSITIDASE-C ISOFORM BETA-1 IS SPECIFICALLY, BUT NOT PREDOMINANTLY, LOCATED IN THE NUCLEUS SO BIOCHEMICAL JOURNAL LA English DT Note ID PROTEIN-KINASE-C; SWISS 3T3 CELLS; GROWTH FACTOR-I; PHOSPHOLIPASE-C; BOVINE BRAIN; GQ; PURIFICATION; METABOLISM; RECEPTOR; MEMBRANE AB The presence of phosphoinositide-mobilizing enzymes has been investigated in purified rat liver nuclei by radiolabelling and by probing with antibodies. A Ca2+-activated phosphoinositidase C (PIC) is present and was shown immunologically to be the beta1 isoform. No gamma- or delta-PIC was found. However, only 5% of the total beta1-PIC isoform is nuclear, with the majority being cytosolic. Galpha(q) and Galpha11 the suggested physiological activators of beta1-PIC, were not present. A Ptdlns4P 5-kinase is also present, which immunologically is shown to be the C isoform. All of these nuclear inositide enzymes still remained after the removal of the nuclear envelope with Triton X-100. consistent with the concept of an intranuclear inositide cycle [Divecha, Banfic and Irvine (1991) EMBO. J. 10, 3207-3214]. C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. RP DIVECHA, N (reprint author), AFRC,INST ANIM PHYSIOL & GENET RES,DEPT BIOCHEM,CAMBRIDGE RES STN,CAMBRIDGE CB2 4AT,ENGLAND. NR 25 TC 171 Z9 171 U1 1 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD FEB 1 PY 1993 VL 289 BP 617 EP 620 PN 3 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KL499 UT WOS:A1993KL49900001 PM 8382043 ER PT J AU ZHANGKECK, ZY BURNS, AL POLLARD, HB AF ZHANGKECK, ZY BURNS, AL POLLARD, HB TI MOUSE SYNEXIN (ANNEXIN-VII) POLYMORPHISMS AND A PHYLOGENETIC COMPARISON WITH OTHER SYNEXINS SO BIOCHEMICAL JOURNAL LA English DT Article ID CALCIUM-CHANNEL ACTIVITY; MEMBRANE-FUSION; CHROMAFFIN GRANULES; BILAYER-MEMBRANES; CDNA SEQUENCE; PROTEIN; IDENTIFICATION; SUBSTRATE; BINDING; DOMAIN AB Two sets of cDNAs encoding mouse synexin were isolated from a liver cDNA library and sequenced. The coding regions of synexin clones show 99% identity. By contrast, the two mouse synexin cDNAs differ in a number of ways in both 5' and 3' non-coding regions. The two sets of cDNA encode a polypeptide of 463 amino acid residues which has a deduced molecular mass of 50 kDa. The amino acid sequence of mouse synexin shows a high degree of similarity to both the unique N-terminal domain and the highly conserved C-terminal domain of previously cloned human synexin. Northern-blot analysis using mouse liver polyadenylated RNA revealed two transcripts of 1.8 kb and 2.6 kb, corresponding to group I and group II respectively. Further hybridization analysis using specific sequences from each set of clones showed that the two sizes of mRNAs differ in the length of the 3' non-coding region which corresponded to the cDNAs. Both mouse liver synexin and recombinant mouse synexin expressed in Escherichia coli reacted after Western-blot analysis with a goat antibody against bovine synexin. Only in the larger group-II cDNAs do we find point mutations leading to amino acid replacements of Ser to Ala at residue 145 in the unique N-terminal domain, and of Ala to Glv at residue 304 in the transition zone between repeats II and III. We conclude from a comparison of mouse, human and Dictyostelium synexins that changes occur predominantly in the hydrophobic N-terminal domain, or, in the C-terminal region at the ends of some predicted alpha-helices. on the hydrophobic face of the amphipathic C-helices. and within a lengthy non-helical domain connecting major repeats II and III. RP ZHANGKECK, ZY (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. NR 21 TC 26 Z9 27 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD FEB 1 PY 1993 VL 289 BP 735 EP 741 PN 3 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KL499 UT WOS:A1993KL49900019 PM 7916616 ER PT J AU KRASNEWICH, DM TIETZE, F KRAUSE, W PRETZLAFF, R WENGER, DA DIWADKAR, V GAHL, WA AF KRASNEWICH, DM TIETZE, F KRAUSE, W PRETZLAFF, R WENGER, DA DIWADKAR, V GAHL, WA TI CLINICAL AND BIOCHEMICAL-STUDIES IN AN AMERICAN CHILD WITH SIALURIA SO BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY LA English DT Article ID LYSOSOMAL STORAGE DISORDER; SIALIC-ACID METABOLISM; CYTOPLASMIC PH; HUMAN-FIBROBLASTS; SALLA DISEASE C1 NIDDKD,MOLEC & CELL BIOL LAB,BETHESDA,MD 20892. SCOTTISH RITE CHILDRENS MED CTR,ATLANTA,GA 30363. THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DIV MED GENET,PHILADELPHIA,PA 19107. RP KRASNEWICH, DM (reprint author), NICHHD,HUMAN GENET BRANCH,BETHESDA,MD 20892, USA. NR 18 TC 14 Z9 14 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0885-4505 J9 BIOCHEM MED METAB B PD FEB PY 1993 VL 49 IS 1 BP 90 EP 96 DI 10.1006/bmmb.1993.1010 PG 7 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA KQ695 UT WOS:A1993KQ69500010 PM 8439453 ER PT J AU HEYES, MP AF HEYES, MP TI METABOLISM AND NEUROPATHOLOGIC SIGNIFICANCE OF QUINOLINIC ACID AND KYNURENIC ACID SO BIOCHEMICAL SOCIETY TRANSACTIONS LA English DT Article; Proceedings Paper CT 644TH MEETING OF THE BIOCHEMICAL SOC CY SEP 16-18, 1992 CL UNIV GLASGOW, GLASGOW, SCOTLAND SP BIOCHEM SOC HO UNIV GLASGOW ID RAT-BRAIN SLICES; CEREBROSPINAL-FLUID; HUNTINGTONS-DISEASE; 3-HYDROXYANTHRANILIC ACID; INDOLEAMINE 2,3-DIOXYGENASE; ALZHEIMERS-DISEASE; CEREBRAL-CORTEX; HIV-1 INFECTION; RHESUS MACAQUES; AMINO-ACIDS RP HEYES, MP (reprint author), NIMH,ANALYT BIOCHEM SECT,CLIN SCI LAB,BETHESDA,MD 20892, USA. NR 50 TC 41 Z9 43 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0300-5127 J9 BIOCHEM SOC T JI Biochem. Soc. Trans. PD FEB PY 1993 VL 21 IS 1 BP 83 EP 89 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KN697 UT WOS:A1993KN69700020 PM 8449360 ER PT J AU BASSER, PJ GRODZINSKY, AJ AF BASSER, PJ GRODZINSKY, AJ TI THE DONNAN MODEL DERIVED FROM MICROSTRUCTURE SO BIOPHYSICAL CHEMISTRY LA English DT Article DE DONNAN; POISSON-BOLTZMANN; HOMOGENIZATION; DOUBLE-LAYER REPULSION; COMPOSITE MEDIUM; CARTILAGE; GEL AB The ideal Donnan potential of an ionized polyelectrolyte medium is shown to be an approximate solution to a system of Poisson-Boltzmann (PB) equations for a periodic array of charged plates in an electrolyte bath. This result, derived using homogenization and scaling methods, demonstrates that the macrocontinuum, thermodynamic Donnan, and statistical mechanical PB models describe the same phenomenon: electrostatic repulsion between fixed-charged groups (albeit at different length scales). The Donnan approximation is accurate at low ionic strength (i.e., where the Debye length is much larger than the separation between charged plates), but is less faithful at physiologic and higher ionic strength. This work also provides a framework for relating theories of electrostatic repulsive interactions formulated at microscopic and macroscopic length scales. C1 MIT,DEPT ELECT ENGN & COMP SCI,CONTINUUM ELECTROMECH GRP,CAMBRIDGE,MA 02139. RP BASSER, PJ (reprint author), NIH,NCRR,BEIP,BLDG 13,RM 3W13,BETHESDA,MD 20892, USA. RI Basser, Peter/H-5477-2011 FU NIAMS NIH HHS [AR33236] NR 20 TC 20 Z9 20 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0301-4622 J9 BIOPHYS CHEM JI Biophys. Chem. PD FEB PY 1993 VL 46 IS 1 BP 57 EP 68 DI 10.1016/0301-4622(93)87007-J PG 12 WC Biochemistry & Molecular Biology; Biophysics; Chemistry, Physical SC Biochemistry & Molecular Biology; Biophysics; Chemistry GA KM992 UT WOS:A1993KM99200007 PM 8443336 ER PT J AU FOSTER, MC LEAPMAN, RD LI, MX ATWATER, I AF FOSTER, MC LEAPMAN, RD LI, MX ATWATER, I TI ELEMENTAL COMPOSITION OF SECRETORY GRANULES IN PANCREATIC-ISLETS OF LANGERHANS SO BIOPHYSICAL JOURNAL LA English DT Article ID ELECTRON-PROBE MICROANALYSIS; X-RAY-MICROANALYSIS; SUBCELLULAR COMPARTMENTS; ENDOCRINE TUMORS; CALCIUM CONTENT; CELL-LINES; INSULIN; ACIDIFICATION; GLUCAGON; SECTIONS AB We have characterized, by electron probe microanalysis, rapidly frozen cultured rat islets at the level of individual secretory granules. Elemental analysis of thin, dried cryosections showed that beta granules could be distinguished by high Zn, Ca, and S, whereas non-beta (mainly alpha) granules contained elevated P and Mg. Although a single granule type predominated in a particular cell, some rebel granules were found in A cells that had the compositional fingerprint of B cell granules. Zn, which was found in millimolar concentrations in B cell granules, was considered a marker for the insulin storage complex. The data indicate that non-B islet cells in the adult pancreas may produce insulin-containing organelles and that, when glucagon and insulin are coexpressed, these hormones are packaged in separate granules. C1 NIDDKD, NATL CTR RES RESOURCES, BIOMED ENGN & INSTRUMENTAT PROGRAM, BETHESDA, MD 20892 USA. NIDDKD, CELL BIOL & GENET LAB, BETHESDA, MD 20892 USA. RP FOSTER, MC (reprint author), SUNY STONY BROOK, SCH MED, DEPT ANESTHESIOL, STONY BROOK, NY 11794 USA. NR 52 TC 72 Z9 72 U1 0 U2 5 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0006-3495 EI 1542-0086 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP 525 EP 532 PG 8 WC Biophysics SC Biophysics GA KN875 UT WOS:A1993KN87500024 PM 8457676 ER PT J AU ABUGO, O RIFKIND, JM AF ABUGO, O RIFKIND, JM TI HEMOGLOBIN-MEMBRANE INTERACTIONS AS PROBED BY NITROBLUE TETRAZOLIUM SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 1 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A308 EP A308 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701767 ER PT J AU AKESON, M RIGAUT, K SHARP, C NEVILLE, DM AF AKESON, M RIGAUT, K SHARP, C NEVILLE, DM TI HIGH EXTRACELLULAR K+ INHIBITS VESICULAR STOMATITIS-VIRUS INFECTION OF BHK CELLS AT A STEP BETWEEN PRIMARY AND SECONDARY TRANSCRIPTION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIMH,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A189 EP A189 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701085 ER PT J AU ARCHER, SJ TORCHIA, DA AF ARCHER, SJ TORCHIA, DA TI TRANSFORMING GROWTH FACTOR-BETA-1 - STRUCTURAL STUDIES BY MULTIDIMENSIONAL HETERONUCLEAR NMR-SPECTROSCOPY SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDR,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A371 EP A371 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702138 ER PT J AU BATENJANY, MM HUANG, C LEVIN, IW AF BATENJANY, MM HUANG, C LEVIN, IW TI BILAYER PACKING CHARACTERISTICS OF MIXED CHAIN PHOSPHOLIPID DERIVATIVES - RAMAN AND DSC STUDIES OF C(18)-C(10)PC AND C(18)-C(10)TMPC SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. UNIV VIRGINIA,SCH MED,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22908. NR 0 TC 0 Z9 0 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A71 EP A71 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700396 ER PT J AU BHATIADEY, N ADELSTEIN, RS DAWID, IB AF BHATIADEY, N ADELSTEIN, RS DAWID, IB TI CLONING OF THE CDNA-ENCODING THE MYOSIN HEAVY CHAIN-B ISOFORM OF XENOPUS NONMUSCLE MYOSIN WITH AN INSERT IN THE HEAD REGION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NICHHD,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A145 EP A145 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700831 ER PT J AU BIVIN, DB KUBOTA, S PEARLSTEIN, R MORALES, MF AF BIVIN, DB KUBOTA, S PEARLSTEIN, R MORALES, MF TI SOME EFFECTS OF CHARGE ON THE EMISSION OF TRYPTOPHAN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UOP,SAN FRANCISCO,CA. PENINSULA LABS INC,BELMONT,CA. NIH,DCRT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A53 EP A53 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700295 ER PT J AU BLUMENTHAL, R AF BLUMENTHAL, R TI A DISSECTION OF STEPS LEADING TO VIRAL ENVELOPE PROTEIN-MEDIATED MEMBRANE-FUSION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A111 EP A111 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700637 ER PT J AU BOYLAN, J WIGGERT, B CHADER, G REED, R HAMILTON, JA AF BOYLAN, J WIGGERT, B CHADER, G REED, R HAMILTON, JA TI BINDING OF FATTY-ACIDS (FA) TO THE INTERPHOTORECEPTOR-RETINOID BINDING-PROTEIN (IRBP) SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 BOSTON UNIV,SCH MED,DEPT BIOPHYS,BOSTON,MA 02118. NEI,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A289 EP A289 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701657 ER PT J AU CASASFINET, JR SOWDER, RC SAKAGUCHI, K APPELLA, E HENDERSON, LE ERICKSON, JW AF CASASFINET, JR SOWDER, RC SAKAGUCHI, K APPELLA, E HENDERSON, LE ERICKSON, JW TI PHYSICOCHEMICAL AND NUCLEIC-ACID BINDING-PROPERTIES OF HIV-1 AND SIV NUCLEOCAPSID PROTEINS AND PEPTIDES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,STRUCT BIOCHEM PROGRAM,FREDERICK,MD 21702. NCI,FCRDC,AIDS VACCINE PROGRAM,FREDERICK,MD 21702. NCI,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A178 EP A178 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701021 ER PT J AU CASASFINET, JR SOWDER, RC YU, X MENDELEYEV, J FENSELAU, C KUN, E ERICKSON, JW HENDERSON, LE AF CASASFINET, JR SOWDER, RC YU, X MENDELEYEV, J FENSELAU, C KUN, E ERICKSON, JW HENDERSON, LE TI 3-NITROSOBENZAMIDE (NOBA) REACTS WITH THE ZINC-FINGER SEQUENCES OF HIV-1 NUCLEOCAPSID PROTEIN (P7) AND PHAGE-T4 GENE 32 PROTEIN (GP32) SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,STRUCT BIOCHEM,FREDERICK,MD 21702. NCI,AIDS VACC,FREDERICK,MD 21702. UMBC,DEPT CHEM,BALTIMORE,MD 21228. SFSU,ENVIR TOXIC & CHEM LAB,TIBURON,CA 94920. NR 0 TC 0 Z9 0 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A125 EP A125 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700714 ER PT J AU CASASFINET, JR AF CASASFINET, JR TI FLUOROMETRIC CHARACTERIZATION OF TRP RESIDUES IN ESCHERICHIA-COLI SINGLE-STRANDED DNA-BINDING (SSB) PROTEIN AND ITS POLY(DT) COMPLEX SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,PRI DYNCORP,STRUCT BIOCHEM PROG,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A54 EP A54 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700301 ER PT J AU CHANTURIA, AN CHERNOMORDIK, LV GAWRISCH, K ZIMMERBERG, J AF CHANTURIA, AN CHERNOMORDIK, LV GAWRISCH, K ZIMMERBERG, J TI INTERACTION OF THE PEPTIDE FRAGMENT 828-848 OF THE CARBOXY-TERMINAL REGION OF THE HIV ENVELOPE GLYCOPROTEIN WITH PLANAR LIPID BILAYERS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A299 EP A299 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701716 ER PT J AU CHAVEZ, MD CARDUCCI, JS BERGER, RL AF CHAVEZ, MD CARDUCCI, JS BERGER, RL TI EFFECT OF CHLORIDE ON THE CO2 BINDING OF HEMOGLOBIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A46 EP A46 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700250 ER PT J AU CHEN, YD BRENNER, B AF CHEN, YD BRENNER, B TI ON THE REGENERATION OF THE ACTIN-MYOSIN POWER STROKE IN CONTRACTING MUSCLE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. UNIV ULM,DEPT GEN PHYSIOL,W-7900 ULM,GERMANY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A25 EP A25 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700130 ER PT J AU CHOI, OH ADELSTEIN, RS BEAVEN, MA AF CHOI, OH ADELSTEIN, RS BEAVEN, MA TI SECRETION FROM BASOPHILIC RBL-2H3 CELLS IS ASSOCIATED WITH PHOSPHORYLATION OF MYOSIN LIGHT-CHAINS BY MYOSIN LIGHT CHAIN KINASE AND PROTEIN-KINASE-C SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A144 EP A144 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700823 ER PT J AU CHUNG, J SCIAKY, N GROSS, DJ AF CHUNG, J SCIAKY, N GROSS, DJ TI EGF BINDING-KINETICS AT THE SINGLE CELL LEVEL SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,CBMB,BETHESDA,MD 20892. UNIV MASSACHUSETTS,PROGRAM MOLEC & CELLULAR BIOL,AMHERST,MA 01003. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A83 EP A83 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700470 ER PT J AU COHEN, JA PARSEGIAN, VA RAU, DC AF COHEN, JA PARSEGIAN, VA RAU, DC TI OSMOTIC-PRESSURE OF 3-DIMENSIONAL ORDERED COLLOIDAL SUSPENSIONS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV PACIFIC,SAN FRANCISCO,CA 94115. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A63 EP A63 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700352 ER PT J AU CONWAY, JF TRUS, BL BOOY, FP NEWCOMB, WW BROWN, JC STEVEN, AC AF CONWAY, JF TRUS, BL BOOY, FP NEWCOMB, WW BROWN, JC STEVEN, AC TI EFFECTS OF RADIATION-DAMAGE ON STRUCTURE OF FROZEN HYDRATED HSV-1 CAPSIDS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,STRUCT BIOL RES LAB,BETHESDA,MD 20892. NIH,DCRT,BETHESDA,MD 20892. UNIV VIRGINIA,HLTH SCI CTR,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,HLTH SCI CTR,CTR CANC,CHARLOTTESVILLE,VA 22908. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A64 EP A64 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700358 ER PT J AU CUDA, G FANANAPAZIR, L EPSTEIN, ND SELLERS, JR AF CUDA, G FANANAPAZIR, L EPSTEIN, ND SELLERS, JR TI ABNORMAL FUNCTION OF BETA-MYOSIN IN HYPERTROPHIC CARDIOMYOPATHY SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. RI Cuda, Giovanni/F-5359-2012 OI Cuda, Giovanni/0000-0001-6313-1866 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A362 EP A362 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702081 ER PT J AU DIMITROV, DS BRODER, CC BERGER, EA BLUMENTHAL, R AF DIMITROV, DS BRODER, CC BERGER, EA BLUMENTHAL, R TI CALCIUM-IONS AND HUMAN CELL COMPONENTS ARE REQUIRED FOR CELL-FUSION MEDIATED BY THE CD4-HIV-1-ENVELOPE GLYCOPROTEIN INTERACTION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A234 EP A234 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701349 ER PT J AU DING, J JACOBOMOLINA, A NANNI, RG LU, X WILLIAMS, RL CLARK, AD HUGHES, SH ARNOLD, E AF DING, J JACOBOMOLINA, A NANNI, RG LU, X WILLIAMS, RL CLARK, AD HUGHES, SH ARNOLD, E TI STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE/DSDNA/FAB COMPLEX - PROTEIN-DNA INTERACTIONS AND STRUCTURE OF THE POLYMERASE ACTIVE-SITE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 RUTGERS STATE UNIV,CTR ADV BIOTECHNOL & MED,PISCATAWAY,NJ 08854. RUTGERS STATE UNIV,DEPT CHEM,PISCATAWAY,NJ 08854. NCI,FREDERICK CANC RES & DEV CTR,ALB BASIC RES PROGRAM,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A350 EP A350 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702016 ER PT J AU DURELL, SR AF DURELL, SR TI INVESTIGATIONS OF SOLVATION FREE-ENERGY USING MOLECULAR-DYNAMICS SIMULATIONS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,MATH BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A177 EP A177 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701015 ER PT J AU EHRLICH, A BARNETT, VA SCHOENBERG, M AF EHRLICH, A BARNETT, VA SCHOENBERG, M TI STOICHIOMETRY OF ALKYLATING AGENT REACTIVITY IN MUSCLE-FIBERS - A FOLLOW-UP-STUDY WITH MORE EFFECTIVE FIBER-BUNDLE SKINNING SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A360 EP A360 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702068 ER PT J AU EIDELMAN, O POLLARD, HB DELAFUENTE, M LEE, G AF EIDELMAN, O POLLARD, HB DELAFUENTE, M LEE, G TI FUSION OF LIPOSOMES INDUCED BY [DES 1-12] LIPOCORTIN-I SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CELL BIOL & GENET LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A187 EP A187 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701073 ER PT J AU FUKUYAMA, R RAPOPORT, SI AF FUKUYAMA, R RAPOPORT, SI TI REGULATION OF GENE-EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN PC12 AND HUMAN LYMPHOID-CELLS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,LNS,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A101 EP A101 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700578 ER PT J AU GALDZICKI, Z FUKUYAMA, R RAPOPORT, SI AF GALDZICKI, Z FUKUYAMA, R RAPOPORT, SI TI EXOGENOUS BETA-AMYLOID PEPTIDE INCREASES MEMBRANE LEAKINESS IN CULTURED PC12 CELLS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,LNS,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A101 EP A101 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700576 ER PT J AU GAMBASSI, G LAKATTA, EG SPURGEON, HA CAPOGROSSI, MC AF GAMBASSI, G LAKATTA, EG SPURGEON, HA CAPOGROSSI, MC TI RATE-DEPENDENT DECREASE OF TWITCH AMPLITUDE DURING HYPERCARBIC ACIDOSIS IN RAT CARDIAC MYOCYTES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,CARDIOVASC SCI LAB,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A41 EP A41 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700224 ER PT J AU GANDJBAKHCHE, A BONNER, R NOSSAL, R AF GANDJBAKHCHE, A BONNER, R NOSSAL, R TI SPATIAL-RESOLUTION OF TIME-RESOLVED LASER IMAGING SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A218 EP A218 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701259 ER PT J AU GAO, JL MURPHY, PM AF GAO, JL MURPHY, PM TI ANALYSIS OF THE LIGAND-BINDING SITE OF THE HUMAN N-FORMYL PEPTIDE RECEPTOR SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAID,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A83 EP A83 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700471 ER PT J AU GARCIACALVO, M KNAUS, HG KACZOROWSKI, GJ GARCIA, ML KEMPNER, E AF GARCIACALVO, M KNAUS, HG KACZOROWSKI, GJ GARCIA, ML KEMPNER, E TI DETERMINATION OF THE TARGET SIZE OF THE CHARYBDOTOXIN RECEPTOR FROM BOVINE AORTIC AND TRACHEAL SMOOTH-MUSCLE BY RADIATION INACTIVATION ANALYSIS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 MERCK RES LABS,RAHWAY,NJ 07065. NIH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A199 EP A199 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701145 ER PT J AU GARLAND, C CHUKNYISKY, P JANZEN, C BEAL, R BUTZOW, J TARIEN, E CLARK, P EICHHORN, G AF GARLAND, C CHUKNYISKY, P JANZEN, C BEAL, R BUTZOW, J TARIEN, E CLARK, P EICHHORN, G TI A MAGNESIUM-ION SWITCH FOR ASSURING FIDELITY IN RNA-SYNTHESIS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A356 EP A356 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702045 ER PT J AU GAWRISCH, K HAN, KH YANG, JS BERGELSON, LD FERRETTI, JA AF GAWRISCH, K HAN, KH YANG, JS BERGELSON, LD FERRETTI, JA TI ENERGETICS OF BINDING OF A HIGHLY AMPHIPATHIC PEPTIDE TO MEMBRANES - LIPID DOMAIN FORMATION AND PEPTIDE STRUCTURE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A60 EP A60 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700333 ER PT J AU GERSHFELD, NL MUDD, CP TAJIMA, K BERGER, RL AF GERSHFELD, NL MUDD, CP TAJIMA, K BERGER, RL TI BILAYER-BILAYER INTERACTIONS IN EQUILIBRIUM DISPERSIONS OF DMPC FROM SPECIFIC-HEAT MEASUREMENTS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD 20892. NCRR,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 3 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A69 EP A69 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700387 ER PT J AU GIBSON, KR VANEK, PG KALOSS, WD COLLIER, GB CONNAUGHTON, JC ANGELICHIO, M LIVI, GP FLEMING, PJ AF GIBSON, KR VANEK, PG KALOSS, WD COLLIER, GB CONNAUGHTON, JC ANGELICHIO, M LIVI, GP FLEMING, PJ TI RECOMBINANT DOPAMINE BETA-HYDROXYLASE WITHOUT SIGNAL SEQUENCE IS BOTH MEMBRANE-BOUND AND SOLUBLE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 GEORGETOWN UNIV,MED CTR,DEPT BIOCHEM,WASHINGTON,DC 20007. NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701. ONCOR INC,GAITHERSBURG,MD 20877. SMITHKLINE BEECHAM,KING OF PRUSSIA,PA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A195 EP A195 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701123 ER PT J AU GUY, HR DURELL, S AF GUY, HR DURELL, S TI MODELS OF THE SLOWPOKE CALCIUM-ACTIVATED POTASSIUM CHANNEL SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,LMMB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A228 EP A228 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701316 ER PT J AU HARRISON, BC MARCHESERAGONA, SP CHENG, N STEVEN, AC JOHNSON, KA AF HARRISON, BC MARCHESERAGONA, SP CHENG, N STEVEN, AC JOHNSON, KA TI KINESIN DECORATION OF THE MICROTUBULE SURFACE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD 20892. PENN STATE UNIV,UNIV PK,PA 16802. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A264 EP A264 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701514 ER PT J AU HAZARD, ES CROUCH, RK WIGGERT, B CHADER, G AF HAZARD, ES CROUCH, RK WIGGERT, B CHADER, G TI FLUORESCENCE TRANSFER IN IRBP SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,CHARLESTON,SC 29425. NEI,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A211 EP A211 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701215 ER PT J AU HENDLER, RW BOSE, SK SHRAGER, RI AF HENDLER, RW BOSE, SK SHRAGER, RI TI MULTIWAVELENGTH STUDIES ON THE KINETICS OF CYTOCHROME AA3 REDUCTION BY CYTOCHROME-C SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,CELL BIOL LAB,BETHESDA,MD 20892. NIH,DCRT,APPL STUDIES LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A103 EP A103 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700588 ER PT J AU HOFRICHTER, J JONES, CM ANSARI, A HENRY, ER SCHAAD, O EATON, WA AF HOFRICHTER, J JONES, CM ANSARI, A HENRY, ER SCHAAD, O EATON, WA TI ROTATIONAL DIFFUSION OF MYOGLOBIN AND HEMOGLOBIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A271 EP A271 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701557 ER PT J AU HOFRICHTER, J CHRISTOPH, GW EATON, WA AF HOFRICHTER, J CHRISTOPH, GW EATON, WA TI HOMOGENEOUS NUCLEATION INITIATES THE FORMATION OF DOMAINS IN HEMOGLOBIN-S GELS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A43 EP A43 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700233 ER PT J AU IWASA, KH AF IWASA, KH TI STRETCH-DEPENDENT MEMBRANE CAPACITANCE OF THE AUDITORY OUTER HAIR CELL SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDCD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A102 EP A102 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700580 ER PT J AU KASIANOWICZ, JJ BEZRUKOV, SM AF KASIANOWICZ, JJ BEZRUKOV, SM TI CURRENT FLUCTUATIONS REVEAL PROTONATION DYNAMICS AND NUMBER OF IONIZABLE RESIDUES IN THE ALPHA-TOXIN CHANNEL SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 ST PETERSBURG NUCL PHYS INST,ST PETERSBURG,RUSSIA. NIH,BETHESDA,MD 20892. UNIV MARYLAND,COLL PK,MD 20742. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A344 EP A344 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701981 ER PT J AU KELLEY, CA YU, JH ADELSTEIN, RS AF KELLEY, CA YU, JH ADELSTEIN, RS TI CYCLIN-P34CDC2 KINASE PHOSPHORYLATES AN ISOFORM OF NONMUSCLE MYOSIN HEAVY CHAIN-B SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 1 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A144 EP A144 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700825 ER PT J AU KIM, MK ZHURKIN, VB JERNIGAN, RL CAMERINIOTERO, RD AF KIM, MK ZHURKIN, VB JERNIGAN, RL CAMERINIOTERO, RD TI RECOMBINATION PROTEIN MEDIATED TRIPLEX DNA - R-FORM DNA SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. RI Jernigan, Robert/A-5421-2012 NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A237 EP A237 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701366 ER PT J AU KNUETTEL, A SCHMITT, JM BARNES, RL KNUTSON, JR AF KNUETTEL, A SCHMITT, JM BARNES, RL KNUTSON, JR TI SPATIAL LOCALIZATION OF ABSORBING OR FLUORESCENT OBJECTS INSIDE TISSUE - PROGRESS USING NEW PHOTON DENSITY WAVE INTERFERENCE TECHNIQUES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCRR,BEIP,BETHESDA,MD 20892. NHLBI,LCB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A221 EP A221 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701275 ER PT J AU KOCSIS, E CERRITELLI, ME TRUS, BL STEVEN, AC AF KOCSIS, E CERRITELLI, ME TRUS, BL STEVEN, AC TI DETERMINATION OF ROTATIONAL SYMMETRY IN MACROMOLECULES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,LSBR,BETHESDA,MD 20892. NIH,CSL,DCRT,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A64 EP A64 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700360 ER PT J AU KOH, E CHENG, L KINSELLA, J FROEHLICH, J AF KOH, E CHENG, L KINSELLA, J FROEHLICH, J TI [CA2+]1 AND SHORTENING IN FRESHLY-ISOLATED SINGLE ARTERIAL SMOOTH-MUSCLE CELLS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,BALTIMORE,MD 21204. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A363 EP A363 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702090 ER PT J AU KRAFT, T SCHNEKENBUHL, S MESSERLI, M YU, LC CHALOVICH, JM BRENNER, B AF KRAFT, T SCHNEKENBUHL, S MESSERLI, M YU, LC CHALOVICH, JM BRENNER, B TI ACTIVATION OF SKINNED SKELETAL-MUSCLE FIBERS BY N-ETHYLMALEIMIDE-MODIFIED-S1 VERSUS CALCIUM-ACTIVATION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV ULM,W-7900 ULM,GERMANY. SWISS FED INST TECHNOL,CH-8093 ZURICH,SWITZERLAND. NIH,SUI,BETHESDA,MD 20892. E CAROLINA UNIV,GREENVILLE,NC 27834. NR 0 TC 4 Z9 4 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A346 EP A346 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701989 ER PT J AU KRUMBIEGEL, M BLUMENTHAL, R GINSBURG, A REMETA, DP AF KRUMBIEGEL, M BLUMENTHAL, R GINSBURG, A REMETA, DP TI THERMODYNAMIC STUDIES OF THE INFLUENZA-VIRUS HEMAGGLUTININ SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A171 EP A171 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700980 ER PT J AU KUKULJAN, M STOJILKOVIC, SS CATT, KJ ROJAS, E AF KUKULJAN, M STOJILKOVIC, SS CATT, KJ ROJAS, E TI VOLTAGE-DEPENDENCE OF CYTOSOLIC [CA-2+] OSCILLATIONS IN GONADOTROPHS - CA-2+ ENTRY THROUGH L-TYPE CHANNELS SUSTAINS ENDOPLASMIC-RETICULUM FUNCTION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NICHHD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A126 EP A126 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700718 ER PT J AU KUROCHKINA, N LEE, B AF KUROCHKINA, N LEE, B TI MONTE-CARLO SEARCH OF CRAMBIN STRUCTURE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A177 EP A177 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701018 ER PT J AU KUSZEWSKI, J GRONENBORN, AM AF KUSZEWSKI, J GRONENBORN, AM TI REFOLDING STUDIES ON A STREPTOCOCCAL PROTEIN-G DOMAIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A174 EP A174 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701000 ER PT J AU LEE, BK AF LEE, BK TI MAXIMUM AND MINIMUM CHANGES IN THE STABILITY OF GLOBULAR-PROTEINS UPON MUTATION THAT ALTERS THE HYDROPHOBIC EFFECT SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A65 EP A65 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700361 ER PT J AU LEIKIN, S RAU, DC PARSEGIAN, VA AF LEIKIN, S RAU, DC PARSEGIAN, VA TI TEMPERATURE-DEPENDENT HYDRATION FORCES MEASURED BETWEEN COLLAGEN TRIPLE HELICES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,PSL DCRT,BETHESDA,MD 20892. NIDDK,LBM,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A270 EP A270 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701547 ER PT J AU LI, H HALLOWS, WH PUNZI, JS MARQUEZ, VE GOLDSTEIN, BM AF LI, H HALLOWS, WH PUNZI, JS MARQUEZ, VE GOLDSTEIN, BM TI NONBONDED INTERACTIONS IN ALCOHOL DEHYDROGENASE-BOUND BETA-METHYLENE SELENAZOLE-4-CARBOXAMIDE ADENINE-DINUCLEOTIDE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV ROCHESTER,MED CTR,DEPT BIOPHYS,ROCHESTER,NY 14642. NCI,LNC,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A57 EP A57 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700317 ER PT J AU LOWY, RJ WHITNALL, MH BLUMENTHAL, R AF LOWY, RJ WHITNALL, MH BLUMENTHAL, R TI INFLUENZA-VIRUS MACROMOLECULAR REDISTRIBUTION OBSERVED BY VIDEO AND ELECTRON-MICROSCOPY DURING LOW PH-INDUCED FUSION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 AFRRI,BETHESDA,MD 20889. NCI,BETHESDA,MD 20889. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A188 EP A188 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701080 ER PT J AU LUSTIG, B COVELL, DG JERNIGAN, RL AF LUSTIG, B COVELL, DG JERNIGAN, RL TI COMBINATORICS OF 3-DIMENSIONAL LATTICE STRUCTURES OF TRANSFER-RNA SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,MATH BIOL LAB,BETHESDA,MD 20892. DYNCORP,PROGRAM RESOURCES INC,BIOMED SUPERCOMP LAB,FREDERICK,MD 21701. RI Jernigan, Robert/A-5421-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A11 EP A11 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700060 ER PT J AU LYUBCHENKO, YL SHLYAKHTENKO, LS APPELLA, E HARRINGTON, RE AF LYUBCHENKO, YL SHLYAKHTENKO, LS APPELLA, E HARRINGTON, RE TI ALTERNATING PYRIMIDINE-PURINE SEQUENCES INCREASE DNA FLEXIBILITY IN THE COMPLEX BETWEEN CRO PROTEIN AND THE LAMBDA-OR3 BINDING-SITE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV NEVADA,DEPT BIOCHEM,RENO,NV 89557. ARIZONA STATE UNIV,DEPT PHYS,TEMPE,AZ 85287. ARIZONA STATE UNIV,DEPT MICROBIOL,TEMPE,AZ 85287. NCI,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A179 EP A179 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701028 ER PT J AU MAHANEY, JE FROEHLICH, JP THOMAS, DD AF MAHANEY, JE FROEHLICH, JP THOMAS, DD TI CORRELATION OF CA-ATPASE PHOSPHORYLATION KINETICS WITH PROTEIN CONFORMATIONAL-CHANGES DETECTED BY TIME-RESOLVED EPR SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV MINNESOTA,SCH MED,DEPT BIOCHEM,MINNEAPOLIS,MN 55455. NIA,BALTIMORE,MD 21224. RI Thomas, David/B-4257-2012 NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A305 EP A305 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701752 ER PT J AU MAKHOV, AM TRUS, BL CONWAY, JF SIMON, MN ZURABISHVILI, TG MESYANZHINOV, VV STEVEN, AC AF MAKHOV, AM TRUS, BL CONWAY, JF SIMON, MN ZURABISHVILI, TG MESYANZHINOV, VV STEVEN, AC TI MOLECULAR-STRUCTURE AND CONFORMATIONAL SWITCHING OF A VIRAL ADHESIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,LSBR,BETHESDA,MD 20892. NIH,CSL,DCRT,BETHESDA,MD 20892. IVANOVSKY INST,MOSCOW 123098,RUSSIA. BROOKHAVEN NATL LAB,UPTON,NY 10973. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A64 EP A64 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700356 ER PT J AU MALINCHIK, S YU, L AF MALINCHIK, S YU, L TI ANALYSIS OF FACTORS THAT AFFECT EQUATORIAL X-RAY-DIFFRACTION INTENSIFIES FROM MUSCLE-FIBERS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,LPB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A27 EP A27 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700142 ER PT J AU MAO, SY METZGER, H AF MAO, SY METZGER, H TI CHEMICAL CROSS-LINKING OF IGE-RECEPTOR COMPLEXES IN RBL-2H3 CELLS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A83 EP A83 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700469 ER PT J AU MINTON, AP AF MINTON, AP TI MACROMOLECULAR CROWDING, CONFINEMENT, STICKINESS, AND THE ORGANIZATION OF CYTOPLASM SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A340 EP A340 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701958 ER PT J AU MOZAYENI, BR NAJEM, ES FERRETTI, JA RICHARDS, FM AF MOZAYENI, BR NAJEM, ES FERRETTI, JA RICHARDS, FM TI A NOVEL INVITRO TRANSLATION SYSTEM FOR PREPARATIVE SCALE CUSTOM PROTEIN-SYNTHESIS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,LBC,STRUCT BIOPHYS SECT,BETHESDA,MD 20892. YALE UNIV,SCH MED,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06510. JOHNS HOPKINS UNIV,DEPT RADIOL,BALTIMORE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A223 EP A223 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701285 ER PT J AU MOZZARELLI, A RIVETTI, C ROSSI, GL HENRY, ER EATON, WA AF MOZZARELLI, A RIVETTI, C ROSSI, GL HENRY, ER EATON, WA TI OXYGEN BINDING TO SINGLE-CRYSTALS OF HEMOGLOBIN IN THE T-QUATERNARY STRUCTURE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. UNIV PARMA,INST BIOCHEM SCI,I-43100 PARMA,ITALY. RI Mozzarelli, Andrea/C-3615-2014 OI Mozzarelli, Andrea/0000-0003-3762-0062 NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A43 EP A43 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700235 ER PT J AU MUDD, CP GERSHFELD, NL BERGER, RL TAJIMA, K AF MUDD, CP GERSHFELD, NL BERGER, RL TAJIMA, K TI A DIFFERENTIAL HEAT-CONDUCTION MICROCALORIMETER FOR SPECIFIC-HEAT MEASUREMENT OF LIPID BILAYERS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCRR,BETHESDA,MD 20892. NIAMS,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A69 EP A69 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700385 ER PT J AU NAJEM, ES MOZAYENI, BR TAMURA, JK WOODS, R COLLETT, MS FERRETTI, JA AF NAJEM, ES MOZAYENI, BR TAMURA, JK WOODS, R COLLETT, MS FERRETTI, JA TI SOLUTION CONFORMATION OF A PEPTIDE BOUND TO ANTIBODY FAB FRAGMENT SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,SCH MED,BALTIMORE,MD 21205. NHLBI,LBL,STRUCT BIOPHYS SECT,BETHESDA,MD 20892. MEDIMMUNE INC,GAITHERSBURG,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A377 EP A377 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702171 ER PT J AU NICHOLSON, LK KAY, LE DELAGLIO, F BAX, A TORCHIA, DA AF NICHOLSON, LK KAY, LE DELAGLIO, F BAX, A TORCHIA, DA TI BACKBONE AND SIDE-CHAIN DYNAMICS OF STAPHYLOCOCCAL NUCLEASE IN SOLUTION AS STUDIED BY PROTON-DETECTED C-13 AND N-15 NMR-SPECTROSCOPY SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,LCP,BETHESDA,MD 20892. NIDR,BRB,BETHESDA,MD 20892. UNIV TORONTO,DEPT MED GENET,TORONTO M5S 1A8,ONTARIO,CANADA. UNIV TORONTO,DEPT BIOCHEM,TORONTO M5S 1A8,ONTARIO,CANADA. UNIV TORONTO,DEPT CHEM,TORONTO M5S 1A8,ONTARIO,CANADA. NR 0 TC 3 Z9 3 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A182 EP A182 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701046 ER PT J AU NIEBYLSKI, CD SALEM, N AF NIEBYLSKI, CD SALEM, N TI A TIME-RESOLVED FLUORESCENCE SPECTROSCOPIC COMPARISON OF PHOSPHOLIPID-BILAYERS CONTAINING MIXED CHAIN POLYUNSATURATED PHOSPHOLIPIDS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAA,DICBR,MEMBRANE BIOCHEM & BIOPHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A68 EP A68 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700379 ER PT J AU NUSSINOV, R FISCHER, D NOREL, R WOLFSON, HJ AF NUSSINOV, R FISCHER, D NOREL, R WOLFSON, HJ TI 3-DIMENSIONAL MATCHING AND DOCKING OF PROTEIN MOLECULES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,FCRDC,PRI DYN CORP,MATH BIOL LAB,FREDERICK,MD 21702. TEL AVIV UNIV,SCH MED,IL-69978 TEL AVIV,ISRAEL. RI Wolfson, Haim/A-1837-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A284 EP A284 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701625 ER PT J AU PAK, CC KRUMBIEGEL, M BLUMENTHAL, R AF PAK, CC KRUMBIEGEL, M BLUMENTHAL, R TI HEMAGGLUTININ CONFORMATIONAL CHANGE IS RATE-LIMITING FOR PR/8 INFLUENZA-ERYTHROCYTE FUSION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,MATH BIOL LAB,MEMBRANE STRUCT & FUNCT SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A188 EP A188 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701082 ER PT J AU PANYUTIN, IG HSIEH, P AF PANYUTIN, IG HSIEH, P TI DYNAMICS OF SPONTANEOUS DNA BRANCH MIGRATION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NATL INST DIABET & DIGEST & KIDNEY DIS,GENET & BIOCHEM BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A283 EP A283 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701620 ER PT J AU PARSEGIAN, VA GERSHFELD, NL AF PARSEGIAN, VA GERSHFELD, NL TI INERT GLUE IN THE SURFACE FORCE APPARATUS - WHERE ARE THE CONTROLS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,DCRT,NIDDK,NIAMS,BETHESDA,MD 20892. NR 0 TC 5 Z9 5 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A222 EP A222 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701279 ER PT J AU PATO, MD PRESTON, YA SELLERS, JR ADELSTEIN, RS AF PATO, MD PRESTON, YA SELLERS, JR ADELSTEIN, RS TI EXPRESSION OF A TRUNCATED FORM OF CHICKEN BRAIN MYOSIN THAT BINDS TO ACTIN IN AN ATP-DEPENDENT MANNER USING THE BACULOVIRUS EXPRESSION SYSTEM SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 1 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A144 EP A144 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700822 ER PT J AU PELTON, JG TORCHIA, DA MEADOW, ND ROSEMAN, S AF PELTON, JG TORCHIA, DA MEADOW, ND ROSEMAN, S TI TAUTOMERIC STATES OF THE ACTIVE-SITE HISTIDINES OF PHOSPHORYLATED AND UNPHOSPHORYLATED E-COLI-IIIGLC USING HETERONUCLEAR 2D NMR TECHNIQUES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDR,BONE RES BRANCH,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,MCCOLLUM PRATT INST,BALTIMORE,MD 21218. NR 0 TC 1 Z9 1 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A372 EP A372 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702139 ER PT J AU PEMRICK, SM STURZENBECKER, LJ KRATZEISEN, C ARBUZUA, P MARKS, MS MEDIN, JA OZATO, K LEVIN, AA HUNZIKER, W GRIPPO, JF AF PEMRICK, SM STURZENBECKER, LJ KRATZEISEN, C ARBUZUA, P MARKS, MS MEDIN, JA OZATO, K LEVIN, AA HUNZIKER, W GRIPPO, JF TI RELATIVE TRANSACTIVATION EFFICIENCIES OF RAR AND RXR CHIMERIC RECEPTORS CONTAINING THE HORMONE BINDING DOMAIN OF THE VITAMIN-D RECEPTOR SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 DEPT INVEST TOXICOL,NUTLEY,NJ. PHARMA RES,BASEL,SWITZERLAND. HOFFMANN LA ROCHE INC,NUTLEY,NJ 07110. NCI,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A278 EP A278 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701592 ER PT J AU PURI, A PAK, CC DIMITROV, DS BRODER, CC BLUMENTHAL, R AF PURI, A PAK, CC DIMITROV, DS BRODER, CC BLUMENTHAL, R TI MECHANISM OF INHIBITION OF CELL-CELL FUSION BETWEEN GP120/41 AND CD4 EXPRESSING CELLS BY CD4 BEARING PLASMA-MEMBRANE VESICLES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NIAID,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A189 EP A189 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701088 ER PT J AU RAGHUNATHAN, G MILES, TM SASISEKHARAN, V AF RAGHUNATHAN, G MILES, TM SASISEKHARAN, V TI MOLECULAR-STRUCTURE OF A DNA TRIPLE HELIX SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NIDDK,BETHESDA,MD 20892. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A11 EP A11 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700057 ER PT J AU REDOWICZ, MJ KORN, ED AF REDOWICZ, MJ KORN, ED TI EFFECTS OF ATP ON THE CONFORMATION OF MYOSIN-II FROM ACANTHAMOEBA-CASTELLANII SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,CELL BIOL LAB,BETHESDA,MD 20892. RI Redowicz, Maria Jolanta/R-4083-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A145 EP A145 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700828 ER PT J AU REMETA, DP MILES, EW GINSBURG, A AF REMETA, DP MILES, EW GINSBURG, A TI DIFFERENTIAL SCANNING CALORIMETRIC STUDIES OF THE TRYPTOPHAN SYNTHASE MULTIENZYME ALPHA-2-BETA-2 COMPLEX SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDKD,BETHESDA,MD 20892. NHLBI,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A175 EP A175 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701003 ER PT J AU RIFKIND, JM ABUGO, O AF RIFKIND, JM ABUGO, O TI OXYRADICAL DAMAGE TO ERYTHROCYTES ASSOCIATED WITH HEMOGLOBIN-MEMBRANE INTERACTIONS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A308 EP A308 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701770 ER PT J AU RIVAS, G MINTON, AP AF RIVAS, G MINTON, AP TI INDEPENDENT DETERMINATION OF CONCENTRATION GRADIENTS OF MULTIPLE COMPONENTS AT SEDIMENTATION EQUILIBRIUM SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A357 EP A357 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702054 ER PT J AU ROTH, BJ WIKSWO, JP AF ROTH, BJ WIKSWO, JP TI RESPONSE OF CARDIAC TISSUE TO ELECTRICAL-STIMULATION FROM A POINT-SOURCE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. VANDERBILT UNIV,NASHVILLE,TN 37232. RI Roth, Bradley/A-4920-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A208 EP A208 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701198 ER PT J AU RUBIN, RJ CHEN, YD AF RUBIN, RJ CHEN, YD TI A SIMPLE EXPRESSION FOR THE TIME-DEPENDENT LOCAL DENSITY OF LIPID FLUOROPHORES IN THE MEMBRANES OF A CELL-CELL FUSION COMPLEX SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A189 EP A189 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701084 ER PT J AU RYSCHON, TW BALABAN, RS AF RYSCHON, TW BALABAN, RS TI P-31-NMRS AND FATIGUE IN RABBIT MUSCLE INVIVO SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,CARDIAC ENERGET LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A107 EP A107 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700610 ER PT J AU SACKETT, DL KOSKKOSICKA, D AF SACKETT, DL KOSKKOSICKA, D TI DETERMINING THE SIZE OF THE ACTIVE SPECIES OF THE RBC CA-2+-ATPASE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT ANESTH,BALTIMORE,MD 21287. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A334 EP A334 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701919 ER PT J AU SASAO, Y KEMPNER, E PODOLSKY, RJ AF SASAO, Y KEMPNER, E PODOLSKY, RJ TI THE ROLE OF TITIN IN LATERAL ORDERING OF MYOSIN-FILAMENTS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A252 EP A252 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701448 ER PT J AU SATIN, LS TAVALIN, SJ SMOLEN, PD AF SATIN, LS TAVALIN, SJ SMOLEN, PD TI ANALYSIS OF HIT CELL CALCIUM CURRENTS USING A BURST-WAVE-FORM VOLTAGE CLAMP COMMAND SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA,DEPT PHARM TOX MED,RICHMOND,VA 23298. NIDDKD,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A229 EP A229 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701320 ER PT J AU SCHAAD, O ZHOU, HX HENRY, ER SZABO, A EATON, WA AF SCHAAD, O ZHOU, HX HENRY, ER SZABO, A EATON, WA TI MOLECULAR-DYNAMICS SIMULATIONS OF LIGAND DISSOCIATION AND REBINDING IN MYOGLOBIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 1 U2 3 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A43 EP A43 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700232 ER PT J AU SCHNEKENBUHL, S KRAFT, T YU, IC BRENNER, B CHALOVICH, JM AF SCHNEKENBUHL, S KRAFT, T YU, IC BRENNER, B CHALOVICH, JM TI CHARACTERIZATION OF NEM-S-1 IN SOLUTION - BINDING-PROPERTIES AND EFFECTS ON ACTOMYOSIN-ATPASE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV ULM,W-7900 ULM,GERMANY. NIH,BETHESDA,MD 20892. E CAROLINA UNIV,GREENVILLE,NC 27834. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A359 EP A359 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702067 ER PT J AU SHAH, AM SPURGEON, H LAKATTA, EG AF SHAH, AM SPURGEON, H LAKATTA, EG TI 8-BROMO CYCLIC-GMP DECREASES MYOFILAMENT CALCIUM RESPONSIVENESS IN RAT MYOCYTES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIA,GRC,BALTIMORE,MD 21224. UWCM,CARDIFF,WALES. NR 0 TC 2 Z9 2 U1 0 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A39 EP A39 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700213 ER PT J AU SHCHERBATKO, AD WETSEL, WC NEGROVILAR, A ARMSTRONG, DL AF SHCHERBATKO, AD WETSEL, WC NEGROVILAR, A ARMSTRONG, DL TI IMMORTAL HYPOTHALAMIC NEURONS EXPRESS A NOVEL VOLTAGE-GATED CALCIUM-CHANNEL SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LABS,RES TRIANGLE PK,NC 27709. NIEHS,MOLEC & INTEGRAT NEUROSCI LABS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A381 EP A381 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702191 ER PT J AU SHIRINSKY, VP VOROTNIKOV, AV BIRUKOV, KG NANAEV, AK SELLERS, JR COLLINGE, M LUKAS, TJ WATTERSON, DM AF SHIRINSKY, VP VOROTNIKOV, AV BIRUKOV, KG NANAEV, AK SELLERS, JR COLLINGE, M LUKAS, TJ WATTERSON, DM TI KINASE RELATED PROTEIN, A PROTEIN WHICH PROMOTES THE ASSEMBLY OF SMOOTH-MUSCLE MYOSIN MINIFILAMENTS IN THE PRESENCE OF ATP SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 CARDIOL RES CTR,MOSCOW,RUSSIA. NHLBI,BETHESDA,MD 20892. VANDERBILT UNIV,DEPT PHARMACOL,NASHVILLE,TN 37240. RI Vorotnikov, Alexander/A-8392-2014 OI Vorotnikov, Alexander/0000-0002-1460-971X NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A29 EP A29 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700149 ER PT J AU SHYLAKHTENKO, LS LYUBCHENKO, YL HARRINGTON, RE APPELLA, E ZHURKIN, VB AF SHYLAKHTENKO, LS LYUBCHENKO, YL HARRINGTON, RE APPELLA, E ZHURKIN, VB TI DNA TWISTING ESTIMATED BY THE ELECTROPHORETIC MOBILITY AT DIFFERENT TEMPERATURES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV NEVADA,DEPT BIOCHEM,RENO,NV 89557. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 2 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A280 EP A280 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701605 ER PT J AU SIDOROVA, NY RAU, DC AF SIDOROVA, NY RAU, DC TI THE OSMOTIC SENSITIVITY OF NETROPSIN ANALOG BINDING TO DNA SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A267 EP A267 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701533 ER PT J AU SIMON, SA MCINTOSH, TJ NEEDHAM, D DISALVO, EA BOROVYAGIN, V GAWRISCH, K AF SIMON, SA MCINTOSH, TJ NEEDHAM, D DISALVO, EA BOROVYAGIN, V GAWRISCH, K TI MECHANISM BY WHICH TANNIC-ACID INDUCES BILAYER ADHESION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,BIOL CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A348 EP A348 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702001 ER PT J AU SLATER, NT FEIN, A TERASAKI, M REESE, TS AF SLATER, NT FEIN, A TERASAKI, M REESE, TS TI CONTINUITY OF ENDOPLASMIC-RETICULUM IN CEREBELLAR PURKINJE-CELLS STUDIED WITH THE FLUORESCENT DYE DII (C-16) SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NORTHWESTERN UNIV,SCH MED,DEPT PHYSIOL,CHICAGO,IL 60611. UNIV CONNECTICUT,STORRS,CT 06268. NIH,BETHESDA,MD 20892. MARINE BIOL LAB,WOODS HOLE,MA 02543. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A347 EP A347 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701998 ER PT J AU STEINBACH, PJ HODOSCEK, M BROOKS, BR AF STEINBACH, PJ HODOSCEK, M BROOKS, BR TI MODELING SOLVATION EFFECTS ON PROTEIN DYNAMICS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,DCRT,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A183 EP A183 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701052 ER PT J AU THOMPSON, KS VINSON, CR SHUMAN, JD FREIRE, E AF THOMPSON, KS VINSON, CR SHUMAN, JD FREIRE, E TI THERMODYNAMIC CHARACTERIZATION OF THE STRUCTURAL STABILITY OF THE BZIP TRANSCRIPTION FACTOR GCN4 SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294. JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218. JOHNS HOPKINS UNIV,CTR BIOCALORIMETRY,BALTIMORE,MD 21218. NCI,BETHESDA,MD 20892. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 1 U2 1 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A175 EP A175 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701004 ER PT J AU TRUS, BL BOOY, FP NEWCOMB, WW BROWN, JC STEVEN, AC AF TRUS, BL BOOY, FP NEWCOMB, WW BROWN, JC STEVEN, AC TI LOCATION OF VP26 IN THE HERPES-SIMPLEX VIRUS CAPSID SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIAMS,LSBR,BETHESDA,MD 20892. NIH,CSL,DCRT,BETHESDA,MD 20892. UNIV VIRGINIA,HLTH SCI CTR,DEPT MICROBIOL,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,HLTH SCI CTR,CTR CANC,CHARLOTTESVILLE,VA 22908. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A64 EP A64 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700355 ER PT J AU TSAO, DHH WANG, LH NIRENBERG, M FERRETTI, JA AF TSAO, DHH WANG, LH NIRENBERG, M FERRETTI, JA TI NMR SOLUTION STRUCTURE OF THE NK-2 HOMEODOMAIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A170 EP A170 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700974 ER PT J AU VANOSDOL, W YE, Q JOHNSON, ML BILTONEN, RL AF VANOSDOL, W YE, Q JOHNSON, ML BILTONEN, RL TI THE EFFECTS OF THE ANESTHETIC DIBUCAINE ON THE KINETICS OF THE GEL-LIQUID CRYSTALLINE TRANSITION OF DPPC MULTILAMELLAR VESICLES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV VIRGINIA,DEPT BIOCHEM,CHARLOTTESVILLE,VA 22903. UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22903. NIH,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A70 EP A70 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700393 ER PT J AU VOGEL, SS CHERNOMORDIK, LV ZIMMERBERG, J AF VOGEL, SS CHERNOMORDIK, LV ZIMMERBERG, J TI CALCIUM-TRIGGERED FUSION OF SEA-URCHIN EGG CORTICAL GRANULES REQUIRES PROTEINS IN ONLY ONE MEMBRANE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD 20892. RI Vogel, Steven/A-3585-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A189 EP A189 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701086 ER PT J AU VOGEL, SS LEIKINA, EA CHERNOMORDIK, LV AF VOGEL, SS LEIKINA, EA CHERNOMORDIK, LV TI PRE-FUSION INTERMEDIATES OF BOTH PH AND CA-2+-TRIGGERED BIOLOGICAL MEMBRANE-FUSION CAN BE REVERSIBLY ARRESTED WITH LYSOLIPIDS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD 20892. RI Vogel, Steven/A-3585-2012 NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A186 EP A186 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701069 ER PT J AU WANG, F REEDY, MC REEDY, MK SELLERS, JR AF WANG, F REEDY, MC REEDY, MK SELLERS, JR TI TROPOMYOSIN STIMULATES THE RATE OF ACTIN-FILAMENTS SLIDING ON LETHOCERUS MYOSIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. DUKE UNIV,MED CTR,DURHAM,NC 27710. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A359 EP A359 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702065 ER PT J AU WEINSTEIN, JN KOHN, KW GREVER, MR VISWANADHAN, VN RUBINSTEIN, LV MONKS, A SCUDIERO, DA WELCH, L KOUTSOUKOS, AD CHIAUSA, A YANG, J SCHIFF, R LICHT, J WITTES, RE PAULL, KD AF WEINSTEIN, JN KOHN, KW GREVER, MR VISWANADHAN, VN RUBINSTEIN, LV MONKS, A SCUDIERO, DA WELCH, L KOUTSOUKOS, AD CHIAUSA, A YANG, J SCHIFF, R LICHT, J WITTES, RE PAULL, KD TI NEURAL COMPUTING IN THE DEVELOPMENT OF NEW THERAPIES FOR CANCER AND AIDS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A244 EP A244 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701400 ER PT J AU WOLF, DE MCKINNON, CA KAPLAN, D STEPHENS, R ROSS, AH AF WOLF, DE MCKINNON, CA KAPLAN, D STEPHENS, R ROSS, AH TI INTERACTION OF GP75 AND GP140TRK IN THE HIGH-AFFINITY NERVE GROWTH-FACTOR RECEPTOR SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545. NCI,FCRDC,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. RI Messier, Claude/A-2322-2008 OI Messier, Claude/0000-0002-4791-1763 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A385 EP A385 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702214 ER PT J AU XU, S BRENNER, B CHALOVICH, JM YU, LC AF XU, S BRENNER, B CHALOVICH, JM YU, LC TI RADIAL ELASTICITIES OF ATTACHED CROSSBRIDGES IN MUSCLE-FIBERS ARE STATE DEPENDENT SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. E CAROLINA UNIV,GREENVILLE,NC 27834. UNIV ULM,W-7900 ULM,GERMANY. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A252 EP A252 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701445 ER PT J AU YAGODIN, S HOLTZCLAW, LA RUSSELL, JT AF YAGODIN, S HOLTZCLAW, LA RUSSELL, JT TI SPATIOTEMPORAL CHARACTERISTICS OF AGONIST-INDUCED CALCIUM WAVES IN ASTROGLIA SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LCMN,BETHESDA,MD. NINCDS,LN,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A328 EP A328 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701885 ER PT J AU YAGODIN, S HOLTZCLAW, LA BARKER, JL RUSSELL, JT AF YAGODIN, S HOLTZCLAW, LA BARKER, JL RUSSELL, JT TI GABA-A RECEPTOR MEDIATED CL-FLUX INDUCES INTRACELLULAR CALCIUM INCREASE IN LHRH SECRETING NEURONAL CELL-LINE SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LCMN,BETHESDA,MD. NINCDS,LN,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A325 EP A325 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701869 ER PT J AU YANCEYWRONA, JE CAMERINIOTERO, RD AF YANCEYWRONA, JE CAMERINIOTERO, RD TI KINETICS DESCRIBING THE SEARCH FOR HOMOLOGY CARRIED OUT BY RECA PROTEIN SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A237 EP A237 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701364 ER PT J AU YOO, SH LEWIS, MS AF YOO, SH LEWIS, MS TI DIMERIZATION AND TETRAMERIZATION PROPERTIES OF THE C-TERMINAL REGION OF CHROMOGRANIN-A SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NCRR,BEIP,BETHESDA,MD 20892. NIDCD,LCB,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A375 EP A375 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51702161 ER PT J AU YOO, SH AF YOO, SH TI PH-DEPENDENT ASSOCIATION OF CHROMOGRANIN-A WITH SECRETORY VESICLE MEMBRANE AND A MEMBRANE-BINDING REGION OF CHROMOGRANIN-A SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDCD,CELLULAR BIOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A195 EP A195 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701118 ER PT J AU YOSHIKAMI, S SAHU, S HAGINS, WA AF YOSHIKAMI, S SAHU, S HAGINS, WA TI IMPROVED METHODS FOR LABELING CELLULAR AQUEOUS COMPARTMENTS WITH FLUORESCENT POLAR DYES SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A221 EP A221 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701277 ER PT J AU YU, LC GILROY, DX MALINCHIK, S XU, S BRENNER, B AF YU, LC GILROY, DX MALINCHIK, S XU, S BRENNER, B TI 2-DIMENSIONAL X-RAY-DIFFRACTION FROM SKINNED RABBIT MUSCLE-FIBERS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 UNIV ULM,W-7900 ULM,GERMANY. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A26 EP A26 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700136 ER PT J AU ZHOU, HX AF ZHOU, HX TI BROWNIAN DYNAMICS STUDY OF THE INFLUENCES OF ELECTROSTATIC INTERACTION AND DIFFUSION ON PROTEIN-PROTEIN ASSOCIATION KINETICS SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A271 EP A271 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701558 ER PT J AU ZIMMERBERG, J AF ZIMMERBERG, J TI ROLE OF LIPIDS AND PROTEIN IN FUSION PORE FORMATION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A112 EP A112 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51700639 ER PT J AU ZIMMERBERG, J BLUMENTHAL, R SARKAR, D CURRAN, M MORRIS, SJ AF ZIMMERBERG, J BLUMENTHAL, R SARKAR, D CURRAN, M MORRIS, SJ TI FORMATION OF MULTIPLE SMALL PORES AND LIPID FLOW IN INFLUENZA HA-MEDIATED MEMBRANE-FUSION SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NICHHD,LTPB,BETHESDA,MD. UMKO,KANSAS CITY,MO. NCI,LMB,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A188 EP A188 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701083 ER PT J AU ZIMMERMAN, SB AF ZIMMERMAN, SB TI ESTIMATION OF EXCLUDED VOLUME EFFECTS UPON MACROMOLECULAR REACTIONS IN THE CYTOPLASM OF ESCHERICHIA-COLI SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NIDDK,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A340 EP A340 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701957 ER PT J AU ZOLKIEWSKI, M GINSBURG, A AF ZOLKIEWSKI, M GINSBURG, A TI TEMPERATURE AND GUANIDINE INDUCED UNFOLDING OF DODECAMERIC GLUTAMINE-SYNTHETASE FROM ESCHERICHIA-COLI SO BIOPHYSICAL JOURNAL LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BIOPHYSICAL SOCIETY PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0006-3495 J9 BIOPHYS J JI Biophys. J. PD FEB PY 1993 VL 64 IS 2 BP A175 EP A175 PN 2 PG 1 WC Biophysics SC Biophysics GA KP517 UT WOS:A1993KP51701006 ER PT J AU JURETIC, D LEE, B TRINAJSTIC, N WILLIAMS, RW AF JURETIC, D LEE, B TRINAJSTIC, N WILLIAMS, RW TI CONFORMATIONAL PREFERENCE FUNCTIONS FOR PREDICTING HELICES IN MEMBRANE-PROTEINS SO BIOPOLYMERS LA English DT Article ID SECONDARY STRUCTURE PREDICTION; AMINO-ACID-SEQUENCE; PHOTOSYNTHETIC REACTION CENTER; RHODOBACTER-SPHAEROIDES R-26; GLOBULAR-PROTEINS; ALPHA-HELICES; RHODOPSEUDOMONAS-VIRIDIS; ESCHERICHIA-COLI; NEURAL NETWORKS; PARAMETERS AB A suite of FORTRAN programs, PREF, is described for calculating preference functions from the data base of known protein structures and for comparing smoothed profiles of sequence-dependent preferences in proteins of unknown structure. Amino acid preferences for a secondary structure are considered as functions of a sequence environment. Sequence environment of amino acid residue in a protein is defined as an average over some physical, chemical, or statistical property of its primary structure neighbors. The frequency distribution of sequence environments in the data base of soluble protein structures is approximately normal for each amino acid type of known secondary conformation. An analytical expression for the dependence of preferences on sequence environment is obtained after each frequency distribution is replaced by corresponding Gaussian function. The preference for the alpha-helical conformation increases for each amino acid type with the increase of sequence environment of buried solvent accessible surface areas. We show that a set of preference functions based on buried surface area is useful for predicting folding motifs in alpha-class proteins and in integral membrane proteins. The prediction accuracy for helical residues is 79% for 5 integral membrane proteins and 74% for 11 alpha-class soluble proteins. Most residues found in transmembrane segments of membrane proteins with known alpha-helical structure are predicted to be indeed in the helical conformation because of very high middle helix preferences. Both extramembrane and transmembrane helices in the photosynthetic reaction center M and L subunits are correctly predicted. We point out in the discussion that our method of conformational preference functions can identify what physical properties of the amino acids are important in the formation of particular secondary structure elements. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT BIOCHEM,BETHESDA,MD 20814. NIH,BETHESDA,MD 20892. RUDJER BOSKOVIC INST,ZAGREB 4301,CROATIA. RP JURETIC, D (reprint author), UNIV SPLIT,DEPT NAT SCI & ARTS,N TESLE 12,SPLIT 580000,CROATIA. NR 68 TC 26 Z9 27 U1 0 U2 0 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0006-3525 J9 BIOPOLYMERS JI Biopolymers PD FEB PY 1993 VL 33 IS 2 BP 255 EP 273 DI 10.1002/bip.360330208 PG 19 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA KL125 UT WOS:A1993KL12500007 PM 8485300 ER PT J AU USDIN, TB BROWNSTEIN, MJ MOSS, B ISAACS, SN AF USDIN, TB BROWNSTEIN, MJ MOSS, B ISAACS, SN TI SP6 RNA-POLYMERASE CONTAINING VACCINIA VIRUS FOR RAPID EXPRESSION OF CLONED GENES IN TISSUE-CULTURE SO BIOTECHNIQUES LA English DT Note ID CELLS; BACTERIOPHAGE-T7; SYSTEM; VECTORS AB A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase. C1 NIAID,VIRAL DIS LAB,BLDG 4 RM 229,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NIMH,BETHESDA,MD 20892. RI Brownstein, Michael/B-8609-2009 NR 13 TC 17 Z9 17 U1 0 U2 0 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD FEB PY 1993 VL 14 IS 2 BP 222 EP & PG 0 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA KL409 UT WOS:A1993KL40900022 PM 8431286 ER PT J AU REID, DM JONES, CE LUO, CY SHULMAN, NR AF REID, DM JONES, CE LUO, CY SHULMAN, NR TI IMMUNOGLOBULINS FROM NORMAL SERA BIND PLATELET VINCULIN AND TALIN AND THEIR PROTEOLYTIC FRAGMENTS SO BLOOD LA English DT Article ID FOCAL ADHESIONS; ALPHA-ACTININ; PROTEIN; IDENTIFICATION; AUTOANTIBODIES; CYTOSKELETON; ANTIBODIES; CLEAVAGE; SEQUENCE; CALPAIN RP REID, DM (reprint author), NIDDKD,CLIN HEMATOL BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 4D51,BETHESDA,MD 20892, USA. NR 33 TC 15 Z9 16 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 1 PY 1993 VL 81 IS 3 BP 745 EP 751 PG 7 WC Hematology SC Hematology GA KK811 UT WOS:A1993KK81100024 PM 8427966 ER PT J AU EYSTER, ME RABKIN, CS HILGARTNER, MW ALEDORT, LM RAGNI, MV SPRANDIO, J WHITE, GC EICHINGER, S DEMOERLOOSE, P ANDES, WA COHEN, AR MANCOJOHNSON, M BRAY, GL SCHRAMM, W HATZAKIS, A LEDERMAN, MM KESSLER, CM GOEDERT, JJ AF EYSTER, ME RABKIN, CS HILGARTNER, MW ALEDORT, LM RAGNI, MV SPRANDIO, J WHITE, GC EICHINGER, S DEMOERLOOSE, P ANDES, WA COHEN, AR MANCOJOHNSON, M BRAY, GL SCHRAMM, W HATZAKIS, A LEDERMAN, MM KESSLER, CM GOEDERT, JJ TI HUMAN IMMUNODEFICIENCY VIRUS-RELATED CONDITIONS IN CHILDREN AND ADULTS WITH HEMOPHILIA - RATES, RELATIONSHIP TO CD4 COUNTS, AND PREDICTIVE VALUE SO BLOOD LA English DT Article ID SERIAL LYMPHOCYTE-CD4 COUNTS; HOMOSEXUAL MEN; SAN-FRANCISCO; NATURAL-HISTORY; HIV-INFECTION; BISEXUAL MEN; AIDS; PROGRESSION; COHORT; TYPE-1 C1 NCI,VIRAL EPIDEMIOL SECT,BETHESDA,MD 20892. CORNELL UNIV,MED CTR,NEW YORK,NY 10021. MT SINAI MED CTR,NEW YORK,NY 10029. HEMOPHILIA CTR WESTERN PENN,PITTSBURGH,PA. CARDEZA FDN,CTR HEMOPHILIA,PHILADELPHIA,PA. UNIV N CAROLINA,CHAPEL HILL,NC 27514. UNIV VIENNA,A-1010 VIENNA,AUSTRIA. HOP CANTONAL GENEVA,CH-1211 GENEVA 4,SWITZERLAND. TULANE UNIV,SCH MED,NEW ORLEANS,LA 70112. CHILDRENS HOSP PHILADELPHIA,PHILADELPHIA,PA. UNIV COLORADO,DENVER,CO 80202. CHILDRENS HOSP,NATL MED CTR,WASHINGTON,DC 20010. UNIV MUNSTER,W-4400 MUNSTER,GERMANY. UNIV ATHENS,ATHENS,GREECE. CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106. GEORGE WASHINGTON UNIV HOSP,WASHINGTON,DC 20037. RP EYSTER, ME (reprint author), PENN STATE UNIV,MILTON S HERSHEY MED CTR,SCH MED,DEPT MED,FOOT,POB 850,HERSHEY,PA 17033, USA. FU NCI NIH HHS [N01-CP-85649] NR 36 TC 38 Z9 39 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD FEB 1 PY 1993 VL 81 IS 3 BP 828 EP 834 PG 7 WC Hematology SC Hematology GA KK811 UT WOS:A1993KK81100035 PM 8427974 ER PT J AU MARON, BJ PELLICCIA, A SPATARO, A GRANATA, M AF MARON, BJ PELLICCIA, A SPATARO, A GRANATA, M TI REDUCTION IN LEFT-VENTRICULAR WALL THICKNESS AFTER DECONDITIONING IN HIGHLY TRAINED OLYMPIC ATHLETES SO BRITISH HEART JOURNAL LA English DT Article ID HYPERTROPHIC CARDIOMYOPATHY; M-MODE; ECHOCARDIOGRAPHY; DIMENSIONS; ENDURANCE; PATTERNS; HEART AB Background - Clinical distinction between athlete's heart and hypertrophic cardiomyopathy in a trained athlete is often difficult. In an effort to identify variables that may aid in this differential diagnosis, the effects of deconditioning on left ventricular wall thickness were assessed in six highly trained elite athletes who had competed in rowing or canoeing at the 1988 Seoul Olympic Games. Each of these athletes showed substantial ventricular septal thickening associated with training (13-15 mm) which resembled that of hypertrophic cardiomyopathy. Methods - The athletes voluntarily reduced their training substantially for 6-34 weeks (mean 13) after the Olympic competition. Echocardiography was performed at peak training and also after deconditioning, and cardiac dimensions were assessed blindly. Results - Maximum ventricular septal thickness was 13.8 (0.9) mm in the trained state and 10.5 (0.5) in the deconditioned state (p < 0.005) (change 15-33%). Conclusions - The finding that deconditioning may be associated with a considerable reduction in ventricular septal thickness in elite athletes over short periods strongly suggests that these athletes had a physiological form of left ventricular hypertrophy induced by training. Such a reduction in wall thickness with deconditioning may help to distinguish between the physiological hypertrophy of athlete's heart and primary pathological hypertrophy (for example, hypertrophic cardiomyopathy) in selected athletes with increased left ventricular wall thickness. C1 NATL OLYMP COMMITTEE,INST SPORTS SCI,DEPT MED,ROME,ITALY. NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. RP MARON, BJ (reprint author), NIH,BLDG 10,ROOM 7B-15,BETHESDA,MD 20892, USA. NR 19 TC 104 Z9 104 U1 0 U2 2 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0007-0769 J9 BRIT HEART J JI Br. Heart J. PD FEB PY 1993 VL 69 IS 2 BP 125 EP 128 PG 4 WC Cardiac & Cardiovascular Systems; History & Philosophy Of Science SC Cardiovascular System & Cardiology; History & Philosophy of Science GA KL067 UT WOS:A1993KL06700007 PM 8435237 ER PT J AU BERMAN, KF DORAN, AR PICKAR, D WEINBERGER, DR AF BERMAN, KF DORAN, AR PICKAR, D WEINBERGER, DR TI IS THE MECHANISM OF PREFRONTAL HYPOFUNCTION IN DEPRESSION THE SAME AS IN SCHIZOPHRENIA - REGIONAL CEREBRAL BLOOD-FLOW DURING COGNITIVE ACTIVATION SO BRITISH JOURNAL OF PSYCHIATRY LA English DT Article ID CEREBROVASCULAR CO2 REACTIVITY; GLUCOSE-METABOLISM; PHYSIOLOGICAL DYSFUNCTION; XENON-133 INHALATION; AFFECTIVE-DISORDERS; MOOD DISORDERS; CORTEX; COMMON; REDUCTION; ATTENTION AB To test the hypothesis that depressed and schizophrenic patients have a common pathophysiological mechanism for hypofunction of the prefrontal cortex ('hypofrontality'), we measured regional cortical blood flow (rCBF) in ten depressed patients, ten patients with schizophrenia, and 20 age- and sex-matched normal controls. Blood flow was measured during three different cognitive conditions: a resting state, a simple number-matching sensorimotor control task, and the Wisconsin Card Sorting test (WCS). The schizophrenic patients had lower prefrontal rCBF during the WCS. There were no differences in global or regional flow between the depressed patients and the normal subjects during any testing condition. Analysis of rCBF lateralisation showed that during the WCS normal subjects had relatively more left parietal blood flow than depressed patients, who had more right parietal blood flow. Since the testing condition that has most consistently revealed hypofrontality in schizophrenia (i.e. the WCS) was not associated with abnormal rCBF in the depressed patients, these data suggest that the pathophysiological mechanisms underlying prefrontal hypofunction in depression and schizophrenia are different. C1 NIMH,INTRAMURAL RES PROGRAM,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. RP BERMAN, KF (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,INTRAMURAL RES PROGRAM,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032, USA. NR 53 TC 100 Z9 102 U1 0 U2 1 PU ROYAL COLLEGE OF PSYCHIATRISTS PI LONDON PA BRITISH JOURNAL OF PSYCHIATRY 17 BELGRAVE SQUARE, LONDON, ENGLAND SW1X 8PG SN 0007-1250 J9 BRIT J PSYCHIAT JI Br. J. Psychiatry PD FEB PY 1993 VL 162 BP 183 EP 192 DI 10.1192/bjp.162.2.183 PG 10 WC Psychiatry SC Psychiatry GA KL120 UT WOS:A1993KL12000006 PM 8435688 ER PT J AU HIRSCH, D AZOURY, R SARIG, S KRUTH, HS AF HIRSCH, D AZOURY, R SARIG, S KRUTH, HS TI COLOCALIZATION OF CHOLESTEROL AND HYDROXYAPATITE IN HUMAN ATHEROSCLEROTIC LESIONS SO CALCIFIED TISSUE INTERNATIONAL LA English DT Article DE ATHEROSCLEROSIS; CALCIFICATION; HYDROXYAPATITE; CHOLESTEROL; FILIPIN ID FILIPIN AB Cholesterol and calcium phosphate, the latter in the form of hydroxyapatite, accumulate in atherosclerotic lesions. In this report, we demonstrate that these organic and inorganic constituents of lesions can accumulate together, closely associated in crystal agglomerates. Using the fluorescent cholesterol probe, filipin, we identified unesterified cholesterol that was associated with calcium granules in tissue sections of lesions. We also have shown that small crystallites of cholesterol can associate with preformed hydroxyapatite crystals in vitro. Scanning electron microscopy coupled with energy-dispersive X-ray analysis demonstrated the physical association of many small crystallites of cholesterol with larger crystals of hydroxyapatite. These small crystallites of cholesterol associated with hydroxyapatite stained with filipin. This contrasted with the lack of filipin staining of unassociated larger cholesterol crystals or hydroxyapatite alone. How cholesterol and calcium come to be closely associated in crystal agglomerates within atherosclerotic lesions remains to be determined. C1 NHLBI,EXPTL ATHEROSCLEROSIS SECT,BLDG 10,ROOM 5N-113,BETHESDA,MD 20892. HEBREW UNIV JERUSALEM,SCH APPL SCI & TECHNOL,CASALI INST APPL CHEM,IL-91904 JERUSALEM,ISRAEL. NR 11 TC 53 Z9 54 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0171-967X J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD FEB PY 1993 VL 52 IS 2 BP 94 EP 98 DI 10.1007/BF00308315 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KJ154 UT WOS:A1993KJ15400006 PM 8382996 ER PT J AU HSING, AW HANSSON, LE MCLAUGHLIN, JK NYREN, O BLOT, WJ EKBOM, A FRAUMENI, JF AF HSING, AW HANSSON, LE MCLAUGHLIN, JK NYREN, O BLOT, WJ EKBOM, A FRAUMENI, JF TI PERNICIOUS-ANEMIA AND SUBSEQUENT CANCER - A POPULATION-BASED COHORT STUDY SO CANCER LA English DT Article DE PERNICIOUS ANEMIA; CANCER; STOMACH CANCER; FOLLOW-UP ID ATROPHIC GASTRITIS; CARCINOID-TUMORS; RISK FACTOR; TRENDS AB Background. Elevated risk of cancers of the stomach, colon, and buccal cavity, as well as of lymphoma and leukemia, have been reported for patients with pernicious anemia in case reports and hospital-based and cross-sectional studies. Methods. A cohort of 2021 men and 2496 women living in the Uppsala health care region in Sweden, discharged with a hospital diagnosis of pernicious anemia from 1965 to 1983, was followed for 20 years for subsequent risk of cancer. Results. A total of 553 cancers were diagnosed among these patients, significantly more than expected based on cancer standardized incidence rates (SIRs) in the general population (SIR = 1.4; 95% confidence interval [CI], 1.2-1.5). Most prominent were excesses for cancer of the stomach (SIR 2.9; 95% CI, 2.4-3.5), esophagus (SIR = 3.2; 95% CI, 1.8-5.2), and pancreas (SIR = 1.7; 95% CI, 1.2-2.4) among men and women; myeloid leukemia among men (SIR 4.4; 95% CI, 1.8-5.2); and multiple myeloma among women (SIR = 2.5; 95% CI, 1.1-4.9). An excess of gastric carcinoid tumors also was evident in this cohort. The risk of stomach cancer was highest in the first year after diagnosis of pernicious anemia (SIR = 7.4; 95% CI, 5.3-10.1), but an increased risk persisted throughout the follow-up period. The risk of esophageal cancer also remained elevated throughout the study period, although the risk of pancreatic cancer dropped off after 5 years. Conclusions. This study confirms the excess risk of gastric carcinoma and carcinoid tumors associated with pernicious anemia, and suggests that the susceptibility state may extend to esophageal and other cancers. C1 UNIV HOSP UPPSALA,DEPT SURG,UPPSALA,SWEDEN. RP HSING, AW (reprint author), NCI,DIV CANC ETIOL,EPIDEMIOL & BIOSTAT PROGRAM,EXECUT PLAZA N,BETHESDA,MD 20892, USA. NR 28 TC 184 Z9 188 U1 1 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 1 PY 1993 VL 71 IS 3 BP 745 EP 750 DI 10.1002/1097-0142(19930201)71:3<745::AID-CNCR2820710316>3.0.CO;2-1 PG 6 WC Oncology SC Oncology GA KM265 UT WOS:A1993KM26500015 PM 8431855 ER PT J AU EWING, MW LIU, SC GNARRA, JR WALTHER, MM MEYERS, CE LINEHAN, WM AF EWING, MW LIU, SC GNARRA, JR WALTHER, MM MEYERS, CE LINEHAN, WM TI EFFECT OF SURAMIN ON THE MITOGENIC RESPONSE OF THE HUMAN PROSTATE CARCINOMA CELL-LINE PC-3 SO CANCER LA English DT Article; Proceedings Paper CT 1ST NATIONAL CONF ON PROSTATE CANCER CY FEB 13-15, 1992 CL SAN FRANCISCO, CA SP AMER CANC SOC, AMER ACAD FAMILY PHYSICIANS, AMER COLL RADIOL, AMER COLL SURGEONS, COMMISS CANC, AMER UROL ASSOC, NATL ASSOC ONCOL SOCIAL WORKERS, NATL ASSOC SOCIAL WORKERS, ONCOL NURSING SOC, SOC SURG ONCOL DE PROSTATIC CARCINOMA; SURAMIN; CELLULAR PROLIFERATION; GENE EXPRESSION ID FIBROBLAST GROWTH-FACTOR; CYTOMETRIC DNA ANALYSIS; FACTOR RECEPTOR; C-MYC; AUTOCRINE STIMULATION; FACTOR TYPE-BETA-2; TRANSFORMED CELLS; CANCER-CELLS; PROLIFERATION; INHIBITION AB Background. Suramin is an anthelmintic drug that recently has been shown to have clinical efficacy in the treatment of patients with some advanced malignancies, including prostate carcinoma. The current study was done to assess the effect of suramin at clinically relevant doses on the growth in culture of a human prostatic carcinoma cell line, PC-3. Methods. The antiproliferative effect of varying doses of suramin on PC-3 was assessed. Northern blot analysis was done to assess the potential changes in genetic expression at different times after the initiation of treatment. Results. Suramin inhibited the proliferation of PC-3 in a dose-related manner (concentration range, 30-300 muM). Compared with fetal calf serum 2%, when the cells were grown in fetal calf serum 10%, higher concentrations of suramin were required to inhibit tritiated thymidine incorporation. When grown in RPMI without supplement, the PC-3 cell number remained the same. When 100 muM suramin was included, the cell number decreased. By contrast, when RPMI was supplemented with insulin, transferrin, and selenium (ITS), PC-3 grew well. The inhibition of the proliferation of PC-3 cells by suramin was decreased when ITS were added to the cells grown under serum-free conditions. Conclusions. These results were consistent with the hypothesis that in vitro inhibition of the growth of PC-3 cells by suramin may be caused, at least in part, by the growth factor antagonism of the drug. In fetal calf serum 2%, the suramin inhibition was reversible after 3 days. If the treatment was extended to 6 days, however, the PC-3 cells were unable to recover. Cell-cycle analysis revealed that, after 6 days of treatment, there was a decrease in the number of cells in G1 that corresponded with an increased number of cells in G2/M. This suggested that critical antineoplastic events were occurring during this time. Molecular analysis did not detect any altered expression of actin, transforming growth factors alpha or beta, or histone compared with untreated control samples. C1 NCI,SURG BRANCH,UROL ONCOL SECT,BLDG 10,ROOM 2B47,BETHESDA,MD 20892. NCI,CLIN PHARMACOL BRANCH,PROGRAM CLIN ONCOL,BETHESDA,MD 20892. NR 46 TC 12 Z9 13 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 1 PY 1993 VL 71 IS 3 SU S BP 1151 EP 1158 DI 10.1002/1097-0142(19930201)71:3+<1151::AID-CNCR2820711438>3.0.CO;2-K PG 8 WC Oncology SC Oncology GA KM674 UT WOS:A1993KM67400037 PM 8428338 ER PT J AU THOMPSON, TC TRUONG, LD TIMME, TL KADMON, D MCCUNE, BK FLANDERS, KC SCARDINO, PT PARK, SH AF THOMPSON, TC TRUONG, LD TIMME, TL KADMON, D MCCUNE, BK FLANDERS, KC SCARDINO, PT PARK, SH TI TRANSGENIC MODELS FOR THE STUDY OF PROSTATE-CANCER SO CANCER LA English DT Article; Proceedings Paper CT 1ST NATIONAL CONF ON PROSTATE CANCER CY FEB 13-15, 1992 CL SAN FRANCISCO, CA SP AMER CANC SOC, AMER ACAD FAMILY PHYSICIANS, AMER COLL RADIOL, AMER COLL SURGEONS, COMMISS CANC, AMER UROL ASSOC, NATL ASSOC ONCOL SOCIAL WORKERS, NATL ASSOC SOCIAL WORKERS, ONCOL NURSING SOC, SOC SURG ONCOL DE PROSTATE CANCER; TRANSGENIC MICE; ORGAN RECONSTITUTION; ONCOGENES; TRANSFORMING GROWTH FACTOR-BETA ID TRANSFORMING GROWTH-FACTOR; MAMMARY-TUMOR VIRUS; EPITHELIAL-MESENCHYMAL INTERACTIONS; UROGENITAL SINUS MESENCHYME; RODENT URINARY-BLADDER; FACTOR-BETA; MESSENGER-RNA; EXPRESSION; CELLS; MICE AB Transgenic model systems provide tools for obtaining information that clarifies important relationships between genetic alterations and carcinogenesis. One such relationship is the induction of specific growth factor activities by dominantly acting oncogenes. Using a ''transgenic organ'' model referred to as mouse prostate reconstitution (MPR) under conditions where the ras and myc oncogenes were introduced using a recombinant retrovirus into both the mesenchymal and epithelial compartments of the urogenital sinus, poorly differentiated prostate cancer (PC) was produced with high frequency (> 90%) in inbred C57BL/6 mice. Time-course studies using northern blotting and immunohistochemical analysis showed that the transition from benign to malignant status invariably was associated with the induction of elevated transforming growth factor-beta 1 (TGF-beta1) expression. Additional immunohistochemical analysis of TGF-beta1 in human PC and benign prostatic hyperplasia (BPH) showed that positive extracellular staining was significantly more extensive in PC compared with BPH. This differential staining pattern was evident in focal areas of PC adjacent to BPH. These findings in both the MPR model system and human PC suggest that elevated TGF-beta1 expression is involved in the progression to malignancy and that its pattern of expression may become a useful marker of PC. Additional studies using transgenic animal models will continue to provide important clinically useful information about PC in man. C1 BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT PATHOL,HOUSTON,TX 77030. NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. RP THOMPSON, TC (reprint author), BAYLOR COLL MED,SCOTT DEPT UROL,6535 FANNIN F427A,HOUSTON,TX 77030, USA. FU NCI NIH HHS [CA50588]; NIDDK NIH HHS [DK43523] NR 45 TC 43 Z9 44 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 1 PY 1993 VL 71 IS 3 SU S BP 1165 EP 1171 DI 10.1002/1097-0142(19930201)71:3+<1165::AID-CNCR2820711440>3.0.CO;2-U PG 7 WC Oncology SC Oncology GA KM674 UT WOS:A1993KM67400039 PM 8428340 ER PT J AU MYERS, C TREPEL, J SARTOR, O COOPER, M RANSON, M TOKO, T LINEHAN, MW AF MYERS, C TREPEL, J SARTOR, O COOPER, M RANSON, M TOKO, T LINEHAN, MW TI ANTIGROWTH FACTOR STRATEGIES SO CANCER LA English DT Article; Proceedings Paper CT 1ST NATIONAL CONF ON PROSTATE CANCER CY FEB 13-15, 1992 CL SAN FRANCISCO, CA SP AMER CANC SOC, AMER ACAD FAMILY PHYSICIANS, AMER COLL RADIOL, AMER COLL SURGEONS, COMMISS CANC, AMER UROL ASSOC, NATL ASSOC ONCOL SOCIAL WORKERS, NATL ASSOC SOCIAL WORKERS, ONCOL NURSING SOC, SOC SURG ONCOL ID GROWTH-FACTOR RECEPTOR; BENIGN PROSTATIC HYPERPLASIA; ACTING SOMATOSTATIN ANALOG; BREAST CANCER-CELLS; SURAMIN-TREATED RAT; EPITHELIAL-CELLS; FACTOR-ALPHA; MORPHOLOGICAL-CHANGES; MONOCLONAL-ANTIBODY; BASEMENT-MEMBRANE C1 NCI,CLIN PHARMACOL BRANCH,BETHESDA,MD 20892. RP MYERS, C (reprint author), NCI,DIV CANC TREATMENT,PROGRAM CLIN ONCOL,SURG BRANCH,UROL ONCOL SECT,BETHESDA,MD 20892, USA. NR 69 TC 10 Z9 10 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD FEB 1 PY 1993 VL 71 IS 3 SU S BP 1172 EP 1178 DI 10.1002/1097-0142(19930201)71:3+<1172::AID-CNCR2820711441>3.0.CO;2-Z PG 7 WC Oncology SC Oncology GA KM674 UT WOS:A1993KM67400040 PM 7679041 ER PT J AU FINLAY, GJ MARSHALL, E MATTHEWS, JHL PAULL, KD BAGULEY, BC AF FINLAY, GJ MARSHALL, E MATTHEWS, JHL PAULL, KD BAGULEY, BC TI INVITRO ASSESSMENT OF N-[2-(DIMETHYLAMINO)ETHYL]ACRIDINE-4-CARBOXAMIDE, A DNA-INTERCALATING ANTITUMOR DRUG WITH REDUCED SENSITIVITY TO MULTIDRUG RESISTANCE SO CANCER CHEMOTHERAPY AND PHARMACOLOGY LA English DT Article ID TUMOR-CELL-LINES; TOPOISOMERASE-II; ADRIAMYCIN-RESISTANT; P-GLYCOPROTEIN; CARCINOMA; LEUKEMIA; ASSAY; FEASIBILITY; SELECTIVITY; PATTERNS AB The successful treatment of cancer requires the identification of new drugs with novel actions. N-[2-(Dimethylamino)ethyl]acridine-4-carboxamide dihydrochloride (DACA) is a topoisomerase II-targeted antitumour drug with curative activity against murine Lewis lung carcinoma. DACA was assessed for novel patterns of growth inhibition using normal and multidrug-resistant human cell lines. Cells were cultured in 96-well microtitre trays and tested against DACA and related topoisomerase-directed drugs, including amsacrine, etoposide and doxorubicin, and drug concentrations for 50% growth inhibition (IC50 or GI50 values) were determined. In a series of Jurkat leukaemia lines characterised as exhibiting atypical multi-drug resistance, DACA was to a large extent capable of overcoming multidrug resistance exhibited towards the other topoisomerase-directed agents. DACA was also tested against the National Cancer Institute 60-tumour-specific cell-line panel (GI50 Values ranging from 420 to 5,400 nM; mean, 2,100 nM) and against a series of primary cultures of surgically excised melanomas (IC50 values ranging from 60 to 1,600 nM; mean, 590 nm). DELTA values (deviations of logarithmic IC50 or GI50 values from the mean) were calculated and compared by correlation analysis. The standard deviation of DELTA values was found to be lower for DACA than for the other topoisomerase II-directed drugs amsacrine, etoposide, doxorubicin and mitozantrone in both the cell lines and the primary cultures. These lower standard deviations appear to have resulted from the reduced susceptibility of DACA to both P-glycoprotein- and topoisomerase II-mediated multidrug-resistance mechanisms occurring naturally in cell lines and primary cultures. C1 UNIV AUCKLAND,SCH MED,CANC RES LAB,AUCKLAND,NEW ZEALAND. NCI,DCT,INFORMAT TECHNOL BRANCH,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. AUCKLAND HOSP,DEPT CLIN ONCOL,AUCKLAND 3,NEW ZEALAND. NR 27 TC 65 Z9 66 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0344-5704 J9 CANCER CHEMOTH PHARM JI Cancer Chemother. Pharmacol. PD FEB PY 1993 VL 31 IS 5 BP 401 EP 406 DI 10.1007/BF00686155 PG 6 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA KK463 UT WOS:A1993KK46300010 PM 8381721 ER PT J AU HAND, PH ROBBINS, PF SALGALLER, ML POOLE, DJ SCHLOM, J AF HAND, PH ROBBINS, PF SALGALLER, ML POOLE, DJ SCHLOM, J TI EVALUATION OF HUMAN CARCINOEMBRYONIC-ANTIGEN (CEA)-TRANSDUCED AND NONTRANSDUCED MURINE TUMORS AS POTENTIAL TARGETS FOR ANTI-CEA THERAPIES SO CANCER IMMUNOLOGY IMMUNOTHERAPY LA English DT Article DE CARCINOEMBRYONIC ANTIGEN; IMMUNOTHERAPY; CEA-TRANSDUCED TUMOR CELLS ID CARCINOMA CELL-LINE; MONOCLONAL-ANTIBODY; RECOMBINANT; EXPRESSION; B72.3; REACTIVITY; FAMILY; GENE; P97 AB The MC-38 C57BL/6 mouse colon adenocarcinoma cell line has been transduced with a retroviral construct containing cDNA encoding the human carcinoembryonic antigen (CEA) gene [Robbins PF, Kantor JA, Salgaller M, Horan Hand P, Fernsten PD, Schlom J (1991) Cancer Res 51: 3657]. Two clones, MC-38-cea1 and MC-38-cea2, expressed high levels of CEA on their cell surface. A third CEA-expressing cell line, MCA-102-cea3, was similarly derived by transduction of the MCA-102 C57BL/6 mouse fibrosarcoma cell line and is described here. In this study, the three CEA-transduced murine tumor cell lines (MC-38-cea1, MC-38-cea2, MCA-102-cea3) were evaluated for their tumorigenic potential, as well as their ability to serve as in vivo model systems for active and passive immunotherapy studies. Parameters that were investigated include tumor growth rate, the antibody response of the host to CEA, and the CEA content of the tumors. The MC-38-cea2 model appeared to be the most appropriate for immunotherapy studies. Biodistribution studies, using an I-125-labeled anti-CEA mAb, demonstrated efficient tumor targeting of MC-38-cea2 tumors in C57BL/6 and athymic mice. RP HAND, PH (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,BLDG 10,ROOM 8807,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 32 TC 29 Z9 29 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-7004 J9 CANCER IMMUNOL IMMUN JI Cancer Immunol. Immunother. PD FEB PY 1993 VL 36 IS 2 BP 65 EP 75 DI 10.1007/BF01754404 PG 11 WC Oncology; Immunology SC Oncology; Immunology GA KH012 UT WOS:A1993KH01200001 PM 8425211 ER PT J AU QUINTKASNER, S CHISHOLM, L DECARVALHO, M PIEMME, J ZIMMERMAN, K BERMAN, A AF QUINTKASNER, S CHISHOLM, L DECARVALHO, M PIEMME, J ZIMMERMAN, K BERMAN, A TI BASIC PRINCIPLES SO CANCER NURSING LA English DT Article C1 VET ADM MED CTR,MARTINSBURG,WV. RP QUINTKASNER, S (reprint author), NIH,DEPT NURSING,CTR CLIN,BETHESDA,MD 20892, USA. NR 4 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0162-220X J9 CANCER NURS JI Cancer Nurs. PD FEB PY 1993 VL 16 IS 1 BP 63 EP 78 PG 16 WC Oncology; Nursing SC Oncology; Nursing GA KR704 UT WOS:A1993KR70400007 PM 8304991 ER PT J AU ADAMSON, PC BOYLAN, JF BALIS, FM MURPHY, RF GODWIN, KA GUDAS, LJ POPLACK, DG AF ADAMSON, PC BOYLAN, JF BALIS, FM MURPHY, RF GODWIN, KA GUDAS, LJ POPLACK, DG TI TIME COURSE OF INDUCTION OF METABOLISM OF ALL-TRANS-RETINOIC ACID AND THE UP-REGULATION OF CELLULAR RETINOIC ACID-BINDING PROTEIN SO CANCER RESEARCH LA English DT Note ID ACUTE PROMYELOCYTIC LEUKEMIA; THERAPEUTIC ANTICANCER AGENTS; 13-CIS-RETINOYL GLUCURONIDES; DIFFERENTIATION THERAPY; PLASMA; IDENTIFICATION; EXPRESSION; INVIVO; CRABP; BILE AB A study of chronic i.v. dosing of all-trans-retinoic acid (all-trans-RA) was performed to determine whether induction of the capacity-limited elimination process for all-trans-RA occurred with long-term drug administration. Because up-regulation of the cellular retinoic acid-binding proteins (CRABP) may act to bind all-trans-RA intracellularly, the amount of CRABP in skin biopsy samples obtained during and following the course of all-trans-RA administration was also determined. Four adult rhesus monkeys received 50 mg/m2 of all-trans-RA by bolus i.v. injection daily for 8 consecutive days and again for one additional dose following a 7-day period without drug. The plasma disappearance curve of all-trans-RA was characterized by a plateau phase, the duration of which decreased during the period of chronic drug administration, followed by a terminal exponential decay phase, which is consistent with a capacity-limited (saturable) elimination process. The V(max) of this process increased from 0.06 mumol/min on the first day to 0.17 mumol/min by the eighth day of all-trans-RA administration, consistent with induction of an enzymatic process. The amount of CRABP measured in skin biopsy specimens was rapidly induced, increasing to approximately 3-fold baseline levels by day 3 of all-trans-RA administration. It remained at this level throughout the period of chronic drug administration but diminished following the 7-day period without drug. These findings suggest that an intermittent schedule of administration for all-trans-RA has potential advantages over a continuous administration schedule. A period of time without drug administration would allow for return of plasma drug clearance toward baseline levels and down-regulation of CRABP, which could result in higher plasma drug concentrations and possibly less cytoplasmic binding of drug. C1 CORNELL UNIV,MED CTR,COLL MED,DEPT PHARMACOL,NEW YORK,NY 10021. RP ADAMSON, PC (reprint author), NCI,PEDIAT BRANCH,BLDG 10,ROOM 13N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. FU NCI NIH HHS [CA09251-02, CA43796] NR 29 TC 79 Z9 79 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1993 VL 53 IS 3 BP 472 EP 476 PG 5 WC Oncology SC Oncology GA KK306 UT WOS:A1993KK30600007 PM 8381046 ER PT J AU BRODER, S CUSHING, M AF BRODER, S CUSHING, M TI TRENDS IN PROGRAM PROJECT GRANT FUNDING AT THE NATIONAL-CANCER-INSTITUTE SO CANCER RESEARCH LA English DT Article C1 NCI,OFF DIRECTOR,BLDG 31,ROOM A1148,BETHESDA,MD 20892. NR 1 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1993 VL 53 IS 3 BP 477 EP 484 PG 8 WC Oncology SC Oncology GA KK306 UT WOS:A1993KK30600008 PM 8425180 ER PT J AU MARAGOS, CM WANG, JM HRABIE, JA OPPENHEIM, JJ KEEFER, LK AF MARAGOS, CM WANG, JM HRABIE, JA OPPENHEIM, JJ KEEFER, LK TI NITRIC-OXIDE NUCLEOPHILE COMPLEXES INHIBIT THE INVITRO PROLIFERATION OF A375 MELANOMA-CELLS VIA NITRIC-OXIDE RELEASE SO CANCER RESEARCH LA English DT Article ID TUMOR TARGET-CELLS; ACTIVATED MACROPHAGES; RIBONUCLEOTIDE REDUCTASE; AGENTS; IRON; DNA; VASODILATORS; NITRATE; ABILITY; GROWTH AB Cell-mediated antitumor effects have, in part, been attributed to the production of NO. Compounds which generate NO might, therefore, be useful in attenuating the growth of tumor cells. Six nitric oxide/nucleophile adducts that release NO spontaneously in solution were tested for their effectiveness in inhibiting DNA synthesis in A375 human melanoma cells. The complexes of NO with spermine, 3-(n-propylamino)propylamine (PAPA/NO), and diethylamine reduced thymidine incorporation by 50% at concentrations of 24, 44, and 128 muM, respectively. The degree of inhibition was, in general, related to the rate and extent of NO release in solution. A melanoma cell clone sensitive to interleukin 1-mediated cytostasis (A375-C6) was no more sensitive to PAPA/NO than a clone resistant to interleukin 1 (A375-C5), suggesting that the differing inhibitory effects of interleukin 1 in the two A375 cell clones are not due to a differential sensitivity to nitric oxide. Oxymyoglobin (125 muM), a known scavenger of NO, restored the ability of A375-C6 cells to incorporate thymidine in the presence of up to 200 muM PAPA/NO. When PAPA/NO was added to a solution of oxymyoglobin, nitrosylmyoglobin was formed, indicating that the protective effect of myoglobin was due to scavenging of NO. The results are consistent with a nitric oxide-mediated mechanism for NO/nucleophile cytostasis and suggest that such compounds may be useful as tools for investigating the role of reactive nitrogen intermediates in cytostasis and cytotoxicity. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC ETIOL,COMPARAT CARCINOGENESIS,CHEM SECT,BLDG 538,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,FREDERICK,MD 21702. RI Keefer, Larry/N-3247-2014 OI Keefer, Larry/0000-0001-7489-9555 FU NCI NIH HHS [N01-CO-74102] NR 40 TC 147 Z9 147 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1993 VL 53 IS 3 BP 564 EP 568 PG 5 WC Oncology SC Oncology GA KK306 UT WOS:A1993KK30600022 PM 8425188 ER PT J AU GREINER, JW ULLMANN, CD NIERODA, C QI, CF EGGENSPERGER, D SHIMADA, S STEINBERG, SM SCHLOM, J AF GREINER, JW ULLMANN, CD NIERODA, C QI, CF EGGENSPERGER, D SHIMADA, S STEINBERG, SM SCHLOM, J TI IMPROVED RADIOIMMUNOTHERAPEUTIC EFFICACY OF AN ANTICARCINOMA MONOCLONAL-ANTIBODY (I-131-CC49) WHEN GIVEN IN COMBINATION WITH GAMMA-INTERFERON SO CANCER RESEARCH LA English DT Article ID CARCINOEMBRYONIC ANTIGEN EXPRESSION; TUMOR-ASSOCIATED GLYCOPROTEIN; RECOMBINANT ALPHA-INTERFERON; COLON-CARCINOMA; THERAPEUTIC EFFICACY; ANTITUMOR-ACTIVITY; IFN-GAMMA; CELLS; ENHANCEMENT; MELANOMA AB The moderately differentiated human colon tumor cell line, HT-29, constitutively expresses low levels of the high molecular weight mucin, tumor-associated glycoprotein 72 (TAG-72), and the M(r) 180,000 carcinoembryonic antigen (CEA) when grown as s.c. tumors in athymic mice. We report that the in vivo administration of gamma-interferon (IFN-gamma) resulted in a time- and dose-dependent increase in both TAG-72 and CEA expression in the HT-29 tumors. Immunohistochemical staining revealed a more homogeneous TAG-72-positive tumor cell population after IFN-gamma. Furthermore, both anti-TAG-72 and anti-CEA monoclonal antibodies (MAbs) showed enhanced localization to the HT-29 tumors in mice treated with IFN-gamma. Using that experimental model, subsequent studies presented evidence showing that the combination of IFN-gamma with I-131-CC49, an anti-TAG-72 MAb, resulted in a statistically significant improvement in therapeutic efficacy when compared with I-131-CC49 alone. For example, treatment with 300 muCi of I-131-CC49 initially suppressed HT-29 tumor growth; however, that reduction in tumor growth was transient as evidenced by the emergence of additional tumor growth at later time points. In contrast, an 8-day treatment with IFN-gamma in combination with 300 muCi I-131-CC49 resulted in sustained suppression of HT-29 tumor growth. Thus, IFN-gamma in vivo can substantially increase the TAG-72 expression in human colon tumor xenografts which leads to an increased tumor localization of anti-TAG-72 MAbs and seems to be responsible for the enhanced antitumor effects when IFN-gamma was combined with I-131-CC49. The results provide further evidence for including a biological response modifier, such as IFN-gamma, which can increase the expression of specific tumor antigens (i.e., TAG-72 and CEA) subsequently leading to a dramatic improvement in the antitumor efficacy of a radionuclide-conjugated MAb. C1 NCI,BIOSTAT DATA MANAGEMENT SECT,BETHESDA,MD 20892. RP GREINER, JW (reprint author), NCI,TUMOR IMMUNOL & BIOL LAB,BETHESDA,MD 20892, USA. NR 41 TC 80 Z9 80 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD FEB 1 PY 1993 VL 53 IS 3 BP 600 EP 608 PG 9 WC Oncology SC Oncology GA KK306 UT WOS:A1993KK30600028 PM 8425194 ER PT J AU KOVACK, V LEE, HK KOVAC, P AF KOVACK, V LEE, HK KOVAC, P TI ENHANCED STRUCTURAL INFORMATION ON OLIGOSACCHARIDES BY SCAN OF LINKED MAGNETIC AND ELECTROSTATIC FIELDS (B/E) AND NEUTRAL GAS COLLISION FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY (FABMS) SO CARBOHYDRATE RESEARCH LA English DT Article ID METHYL BETA-GLYCOSIDES AB Homo- and hetero-oligosaccharides 1-27, composed of hexopyranose, deoxyhexopyranose, deoxyfluorohexopyranose, and 2-acetamido-2-deoxyhexopyranose units, have been examined by fast-atom-bombardment mass spectrometry. Scans by linked magnetic and electrostatic (B/E) fields of quasi-molecular [M + H]+ ions, or scans combined with helium collision, gave rise to structurally significant ions. The information thus obtained aids significantly in the sequence analysis of oligosaccharides without derivatization. C1 NIDDK,BETHESDA,MD 20892. RP KOVACK, V (reprint author), SLOVAK ACAD SCI,INST CHEM,DUBRAVSKA CESTA,CS-84238 BRATISLAVA,CZECHOSLOVAKIA. NR 15 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0008-6215 J9 CARBOHYD RES JI Carbohydr. Res. PD FEB 1 PY 1993 VL 239 BP 61 EP 70 DI 10.1016/0008-6215(93)84203-I PG 10 WC Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA KL211 UT WOS:A1993KL21100005 PM 8458002 ER PT J AU STARK, AA ZEIGER, E PAGANO, DA AF STARK, AA ZEIGER, E PAGANO, DA TI GLUTATHIONE METABOLISM BY GAMMA-GLUTAMYL-TRANSPEPTIDASE LEADS TO LIPID-PEROXIDATION - CHARACTERIZATION OF THE SYSTEM AND RELEVANCE TO HEPATOCARCINOGENESIS SO CARCINOGENESIS LA English DT Article ID GLUTAMYL-TRANSFERASE TRANSPEPTIDASE; SISTER-CHROMATID EXCHANGES; RENAL-CELL CARCINOMA; FERRIC NITRILOTRIACETATE; PEROXISOME PROLIFERATOR; SALMONELLA-TYPHIMURIUM; PRENEOPLASTIC LESIONS; OXYGEN; IRON; DNA AB Glutathione (GSH)-driven lipid peroxidation (LPO) in vitro was catalyzed by gamma-glutamyltranspeptidase (GGT; EC 2.3.2.2.). The reaction required iron, iron chelators and oxygen, was accelerated by glycylglycine (gly)2, a GGT enhancer, and was inhibited by the GGT inhibitors serine-borate and acivicin. LPO occurred at rat plasma concentrations of GSH and transferrin, and in the presence of putative physiological chelators such as citrate and ADP. GSH-driven LPO was inhibited by butylated hydroxytoluene, but not by catalase, peroxidase or superoxide dismutase. These results suggest that metabolism of GSH initiated by GGT may lead to oxidative damage. Such oxidative damage may be induced in vivo by GSH in proximity to GGT-rich preneoplastic foci in rat liver. C1 NIEHS,EXPTL CARCINOGENESIS & MUTAGENESIS BRANCH,RES TRIANGLE PK,NC 27709. RP STARK, AA (reprint author), TEL AVIV UNIV,DEPT BIOCHEM,IL-69978 TEL AVIV,ISRAEL. NR 59 TC 79 Z9 82 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1993 VL 14 IS 2 BP 183 EP 189 DI 10.1093/carcin/14.2.183 PG 7 WC Oncology SC Oncology GA KM153 UT WOS:A1993KM15300003 PM 8094645 ER PT J AU STRICKLAND, JE DLUGOSZ, AA HENNINGS, H YUSPA, SH AF STRICKLAND, JE DLUGOSZ, AA HENNINGS, H YUSPA, SH TI INHIBITION OF TUMOR-FORMATION FROM GRAFTED MURINE PAPILLOMA CELLS BY TREATMENT OF GRAFTS WITH STAUROSPORINE, AN INDUCER OF SQUAMOUS DIFFERENTIATION SO CARCINOGENESIS LA English DT Article ID PROTEIN-KINASE-C; MOUSE EPIDERMAL-CELLS; TERMINAL DIFFERENTIATION; ORNITHINE DECARBOXYLASE; POTENT INHIBITOR; PROMOTERS; SKIN; KERATINOCYTES; CARCINOGENESIS; INDUCTION AB The microbial alkaloid staurosporine induces responses associated with protein kinase C activation, resulting in terminal differentiation in cultures of both normal and neoplastic mouse epidermal cells. As a cancer chemotherapy model, we treated grafts of mouse epidermal tumor cell lines 308 and SP-1 repeatedly with staurosporine. A dose-dependent inhibition of tumor formation, maximal at 0.025 nmol per treatment, was observed. Higher and lower doses were less effective, suggesting a specific target f or staurosporine action. A single, low-dose treatment 2 weeks after grafting also markedly reduced tumor formation. Although in vitro evidence suggests that staurosporine-induced terminal squamous differentiation results from activation of protein kinase C, we found no inhibition of tumor growth in similar studies with the protein kinase C activator 12-0-tetradecanoylphorbol-13-acetate. These results indicate that staurosporine is an effective antitumor agent for eradicating squamous cell tumors in vivo. RP STRICKLAND, JE (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BETHESDA,MD 20892, USA. NR 25 TC 27 Z9 27 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1993 VL 14 IS 2 BP 205 EP 209 DI 10.1093/carcin/14.2.205 PG 5 WC Oncology SC Oncology GA KM153 UT WOS:A1993KM15300007 PM 8435862 ER PT J AU HECHT, SS TRUSHIN, N REIDQUINN, CA BURAK, ES JONES, AB SOUTHERS, JL GOMBAR, CT CARMELLA, SG ANDERSON, LM RICE, JM AF HECHT, SS TRUSHIN, N REIDQUINN, CA BURAK, ES JONES, AB SOUTHERS, JL GOMBAR, CT CARMELLA, SG ANDERSON, LM RICE, JM TI METABOLISM OF THE TOBACCO-SPECIFIC NITROSAMINE 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE IN THE PATAS MONKEY - PHARMACOKINETICS AND CHARACTERIZATION OF GLUCURONIDE METABOLITES SO CARCINOGENESIS LA English DT Article ID MASS-SPECTROMETRIC ANALYSIS; CHEMICAL CARCINOGENESIS; F344 RATS; N-NITROSAMINES; TISSUE DISTRIBUTION; HUMAN CANCER; CLARA CELLS; N'-NITROSONORNICOTINE; DNA; LUNG AB The metabolism of the tobacco-specific nitrosamine 4-(methyhnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) was examined in the patas monkey, in order to provide further information about NNK metabolic pathways in primates. Female patas monkeys were given i.v. injections of [5-H-3]NNK, and metabolites in serum and urine were analyzed by HPLC. Metabolism by alpha-hydroxylation of NNK was rapid and extensive, and the products of this pathway, 4-hydroxy-4-(3-pyridyl)butyric acid and 4-oxo-4-(3-pyridyl) butyric acid, accounted for a relatively large proportion of serum and urinary metabolites at all time points. This is significant because the formation of these products is associated with modification of DNA by NNK. The other major metabolic pathway was carbonyl reduction to 4-(methytnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), which detected both unconjugated and diastereomeric O-glucuronides. One of these glucuronides had been previously identified in rat urine, but the other diastereomer, which was the more prevalent of the two in serum and urine, had not been observed in studies of NNK metabolism in rodents. It was characterized by its spectral properties, by enzymatic hydrolysis to NNAL, and by derivatization of the released NNAL enantiomer with (R)-(+)-alpha-methylbenzylisocyanate. The two NNAL glucuronides accounted for 15-20% of the urinary metabolites in monkeys given 0.1 mug/kg NNK, which is similar to a smoker's dose, suggesting their use as dosimeters of NNK exposure in humans. Pharmacokinetic parameters were consistent with those observed in previous studies of nitrosamines, and varied predictably with body weight of five species. The results of this study have provided new insights relevant to assessing human metabolism of NNK. C1 NATL CTR RES RESOURCES,VET RESOURCES PROGRAM,BETHESDA,MD 20892. SMITHKLINE BEECHAM CO,PHILADELPHIA,PA 19406. NCI,COMPARAT CARCINOGENESIS LAB,FREDERICK,MD 21702. RP HECHT, SS (reprint author), AMER HLTH FDN,DIV CHEM CARCINOGENESIS,VALHALLA,NY 10595, USA. OI Hecht, Stephen/0000-0001-7228-1356 FU NCI NIH HHS [CA-44377, CA-46535] NR 32 TC 84 Z9 84 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1993 VL 14 IS 2 BP 229 EP 236 DI 10.1093/carcin/14.2.229 PG 8 WC Oncology SC Oncology GA KM153 UT WOS:A1993KM15300011 PM 8435864 ER PT J AU WEST, RW BARRETT, JC AF WEST, RW BARRETT, JC TI INACTIVATION OF A TUMOR SUPPRESSOR FUNCTION IN IMMORTAL SYRIAN-HAMSTER CELLS BY N-METHYL-N'-NITRO-N-NITROSOGUANIDINE AND BY 5-AZA-2'-DEOXYCYTIDINE SO CARCINOGENESIS LA English DT Article ID V-HA-RAS; NEOPLASTIC TRANSFORMATION; EMBRYO CELLS; MUTATIONS; GENES; CHROMOSOME-5Q21; IDENTIFICATION; PROGRESSION; PROTEIN AB Clonal lines of immortal Syrian hamster cells were previously isolated that either suppressed (supB+) tumorigenicity in hybrids with a malignant hamster cell line (BP6T) or had lost this suppression ability (supB-). Neither line was tumorigenic or showed anchorage-independent growth in normal growth medium. SupB- cells, but not supB+ cells, grew in agar supplemented with the growth factors EGF. PDGF and insulin (EPI), providing a selective assay for the supB- phenotype. After treatment of supB+ cells with either N-methyl-N'-nitro-N-nitrosoguanidine (10-300 ng/ml) or 5-aza-2'-deoxycytidine (25-250 ng/ml), and an expression period of 4-8 weeks, a dose-dependent increase in altered cells that grew in agar supplemented with EPI was observed. Cell lines derived from colonies in agar showed persistent EPI-stimulated growth in agar, and decreased suppression of growth in agar for hybrids with BP6T cells. Thus, carcinogen-induced loss of the tumor suppressor phenotype has been demonstrated. C1 NIEHS,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709. RP WEST, RW (reprint author), NATL CTR TOXICOL RES,DIV BIOCHEM TOXICOL,JEFFERSON,AR 72079, USA. NR 27 TC 108 Z9 108 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0143-3334 J9 CARCINOGENESIS JI Carcinogenesis PD FEB PY 1993 VL 14 IS 2 BP 285 EP 289 DI 10.1093/carcin/14.2.285 PG 5 WC Oncology SC Oncology GA KM153 UT WOS:A1993KM15300020 PM 7679613 ER PT J AU WILSON, JB FERGUSON, MWJ JENKINS, NA LOCK, LF COPELAND, NG LEVINE, AJ AF WILSON, JB FERGUSON, MWJ JENKINS, NA LOCK, LF COPELAND, NG LEVINE, AJ TI TRANSGENIC MOUSE MODEL OF X-LINKED CLEFT-PALATE SO CELL GROWTH & DIFFERENTIATION LA English DT Article ID MURINE LEUKEMIA-VIRUS; EARLY EMBRYONIC DEATH; EPSTEIN-BARR-VIRUS; INSERTIONAL MUTAGENESIS; LOCUS LEADS; MICE; GENE; SEQUENCES; MUTATION; INACTIVATION AB A transgenic mouse line, PyLMP.5, exhibited a sex-linked lethality not observed in any other lines expressing the transgene. In this unique line, the transgene integrated into the X chromosome, yielding a simple tandem duplication of the insert sequences with minimal, if any, additional rearrangement of the cellular sequences. The predominant phenotype was a cleft secondary palate and neonatal lethality in males. Survival of females was dependent on the mouse strain background. The disrupted cellular sequences have been mapped to the proximal region of the mouse X chromosome. The disrupted locus may represent the mouse counterpart to a human locus mutated in an X-linked cleft secondary palate syndrome. C1 PRINCETON UNIV,DEPT MOLEC BIOL,PRINCETON,NJ 08544. UNIV GLASGOW,ROBERTSON INST BIOTECHNOL,DEPT GENET,GLASGOW G11 5JS,SCOTLAND. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702. UNIV MANCHESTER,DEPT CELL & STRUCT BIOL,MANCHESTER M13 9PL,LANCS,ENGLAND. RI Wilson, Joanna/B-4466-2009 FU NCI NIH HHS [CA38757-04, CA49271-02, N01-CO-74101] NR 27 TC 27 Z9 27 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 1044-9523 J9 CELL GROWTH DIFFER JI Cell Growth Differ. PD FEB PY 1993 VL 4 IS 2 BP 67 EP 76 PG 10 WC Cell Biology SC Cell Biology GA KL923 UT WOS:A1993KL92300002 PM 8494785 ER PT J AU SIPE, HJ BUCCALDERON, P ROBERFROID, M MASON, RP AF SIPE, HJ BUCCALDERON, P ROBERFROID, M MASON, RP TI IDENTIFICATION OF THE FREE-RADICAL FORMED BY ADDITION OF HYDROXYL RADICAL TO DEHYDROALANINE COMPOUNDS SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE HYDROXYL RADICAL; ELECTRON SPIN RESONANCE; CAPTO-DATIVE; FENTON REACTION; SPIN TRAPPING ID ELECTRON-SPIN RESONANCE; N-ACYL DEHYDROALANINES; MEDIATED PROCESSES; TUMOR PROMOTION; INHIBITION AB N-substituted dehydroalanines, a class of compounds with both acceptor and donor substituents (ADs), react with and scavenge oxygen radicals. Interest in these compounds is based on their potential to lessen the cardiotoxicity of drugs with antineoplastic activity such as Adriamycin. The reactivity of these compounds with hydroxyl radical is evident from their inhibition of hydroxyl radical adduct formation. ESR spin trapping studies of the species formed by reaction of the AD series of compounds with the hydroxyl radical are reported here for the first time. ESR results show that hydroxyl radical attack on the capto-dative site of the AD compounds produces the predicted carbon-centered free radical. C1 NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709. HAMPDEN SYDNEY COLL,DEPT CHEM,HAMPDEN SYDNEY,VA 23943. UNIV CATHOLIQUE LOUVAIN,UNITE BIOCHIM TOXICOL & CANCEROL,B-1200 BRUSSELS,BELGIUM. NR 24 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PD FEB PY 1993 VL 86 IS 2 BP 93 EP 102 DI 10.1016/0009-2797(93)90114-E PG 10 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA KT152 UT WOS:A1993KT15200001 PM 8383583 ER PT J AU FUJITA, K OHTA, K OKABE, Y ISHIZU, T YAMAMOTO, T MINAMIURA, N PITHA, J AF FUJITA, K OHTA, K OKABE, Y ISHIZU, T YAMAMOTO, T MINAMIURA, N PITHA, J TI SELECTIVE PREPARATION OF SUBSTITUTED MALTOOLIGOSACCHARIDES THROUGH ENZYMATIC-HYDROLYSIS OF SUBSTITUTED BETA-CYCLODEXTRINS BY BACTERIAL ALPHA-AMYLASE (SACCHARIFYING TYPE) - A NOVEL METHOD FOR DETERMINING REGIOCHEMICAL STRUCTURE OF DISUBSTITUTED BETA-CYCLODEXTRIN SO CHEMISTRY LETTERS LA English DT Article AB Specifically substituted maltooligosaccharides were prepared through the enzymatic hydrolysis of beta-cyclodextrin derivatives by bacterial alpha-amylase (saccharifying type). C1 FUKUYAMA UNIV, FAC PHARM & PHARMACEUT SCI, FUKUYAMA 72902, JAPAN. FUKUYAMA UNIV, FAC ENGN, FUKUYAMA 72902, JAPAN. OSAKA CITY UNIV, FAC SCI, SUMIYOSHI KU, OSAKA 558, JAPAN. NIA, GRC, BALTIMORE, MD 21224 USA. RP FUJITA, K (reprint author), NAGASAKI UNIV, FAC PHARMACEUT SCI, BUNKYO MACHI, NAGASAKI 852, JAPAN. NR 5 TC 0 Z9 0 U1 0 U2 0 PU CHEMICAL SOC JAPAN PI TOKYO PA 1-5 KANDA-SURUGADAI CHIYODA-KU, TOKYO, 101-8307, JAPAN SN 0366-7022 EI 1348-0715 J9 CHEM LETT JI Chem. Lett. PD FEB PY 1993 IS 2 BP 303 EP 306 PG 4 WC Chemistry, Multidisciplinary SC Chemistry GA KN049 UT WOS:A1993KN04900030 ER PT J AU BANG, KM GERGEN, PJ KRAMER, R COHEN, B AF BANG, KM GERGEN, PJ KRAMER, R COHEN, B TI THE EFFECT OF PULMONARY IMPAIRMENT ON ALL-CAUSE MORTALITY IN A NATIONAL COHORT SO CHEST LA English DT Article ID LUNG-FUNCTION; RESPIRATORY SYMPTOMS; DISEASE; SMOKERS; ADULTS; VOLUME; AGE AB The association between pulmonary impairment and all-cause mortality was investigated among white subjects in a follow-up study of a large national cohort. Pulmonary function was measured during the National Health and Nutrition Examination Survey (NHANESI) (1971 to 1975); subsequent mortality information was obtained from the 1987 NHANES I Epidemiologic Follow-up Study (1982 to 1987). Of 4,764 white sample persons, ages 25 to 74 years examined during NHANES I, 658 (13.8 percent) were identified as having pulmonary impairment defined as a FEV1/FVC less-than-or-equal-to 69 percent. A total of 743 (15.6 percent) sample persons died during the follow-up period. The association between pulmonary impairment and all-cause mortality was examined for male and female subjects separately using the Cox proportional hazards model controlling for age, smoking, educational level, body mass index, and respiratory diseases. The analysis suggests that reduced FEV1 percent percent predicted was a significant risk factor for mortality among both sexes, and the FEV1/FVC ratio was significantly associated with all-cause mortality among male subjects only. C1 MASSACHUSETTS STATE DEPT PUBL HLTH,BOSTON,MA. NATL CTR HLTH STAT,HYATTSVILLE,MD 20782. NIAID,BETHESDA,MD 20892. NR 32 TC 72 Z9 73 U1 0 U2 3 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD FEB PY 1993 VL 103 IS 2 BP 536 EP 540 DI 10.1378/chest.103.2.536 PG 5 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA KL712 UT WOS:A1993KL71200044 PM 8432150 ER PT J AU BRODY, AR BONNER, JC BADGETT, A AF BRODY, AR BONNER, JC BADGETT, A TI RECOMBINANT INTERFERON-GAMMA REDUCES PDGF-INDUCED LUNG FIBROBLAST GROWTH BUT STIMULATES PDGF PRODUCTION BY ALVEOLAR MACROPHAGES INVITRO SO CHEST LA English DT Article RP BRODY, AR (reprint author), NIEHS,PULM PATHOBIOL LAB,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD FEB PY 1993 VL 103 IS 2 SU S BP S121 EP S122 DI 10.1378/chest.103.2_Supplement.121S-a PG 2 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA KM537 UT WOS:A1993KM53700024 PM 8428532 ER PT J AU YANG, YH JIANG, ZF CHEN, XN JIANG, QB ZHANG, GR SHEN, XZ PENG, H SCHNEERSON, R ROBBINS, JB AF YANG, YH JIANG, ZF CHEN, XN JIANG, QB ZHANG, GR SHEN, XZ PENG, H SCHNEERSON, R ROBBINS, JB TI COUNTERCURRENT IMMUNOELECTROPHORESIS FOR DIAGNOSIS OF ACUTE BACTERIAL PNEUMONIA IN CHINESE CHILDREN SO CHINESE MEDICAL JOURNAL LA English DT Article ID INFLUENZAE TYPE-B; COUNTERIMMUNOELECTROPHORESIS; AGGLUTINATION; ANTIGENS; WORLD AB Countercurrent immunoelectrophoresis (CIE) with H. influenzal type b(Hib) and Pneumococci Omni antisera was performed on serum and concentrated urine and pleural fluid samples from 100 patients with acute pneumonia in Beijing Children's Hospital. Thirty-one patients were investigated by bacteriologic techniques (blood culture and pleural fluid culture). CIE was positive in 29 / 100 (29%) of cases for Hib, 13 / 100 (13%) of cases for pneumococci. Overall, bacterial causes of pneumonia were diagnosed by CIE in 41 / 100 (41%) cases. Antigens were detected in 5 / 90 (5.6%) serum samples, in 36 / 87 (41.5%) concentrated urine samples, and in 1 / 2 (50%) samples of pleural fluid. Only one Hib strain was found by pleural fluid culture. The contamination rate of bacterial culture in this group was high (5 / 31 cases, 16.1 %). Therefore, traditional bacteriologic techniques are of very limited value for diagnosing pneumonia in Chinese children. The results showed that bacterial pathogen, especially Hib and Pneumococci, are very common and important causes of pediatric pneumonia in China. C1 BEIJING CHILDRENS HOSP,DIV PULM,BEIJING 100045,PEOPLES R CHINA. BEIJING CHILDRENS HOSP,ICU,BEIJING 100045,PEOPLES R CHINA. NICHHD,DEV & MOLEC IMMUN LAB,BETHESDA,MD. RP YANG, YH (reprint author), BEIJING PEDIAT INST,MICROBIOL & IMMUNOL LAB,BEIJING 100045,PEOPLES R CHINA. NR 15 TC 6 Z9 12 U1 0 U2 1 PU CHINESE MEDICAL ASSOCIATION PI BEIJING PA 42 DONGSI XIDAJIE, BEIJING 100710, PEOPLES R CHINA SN 0366-6999 J9 CHINESE MED J-PEKING JI Chin. Med. J. PD FEB PY 1993 VL 106 IS 2 BP 105 EP 109 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA KW870 UT WOS:A1993KW87000005 PM 8504692 ER PT J AU LIEBSON, PR GRANDITS, G PRINEAS, R DIANZUMBA, S FLACK, JM CUTLER, JA GRIMM, R STAMLER, J AF LIEBSON, PR GRANDITS, G PRINEAS, R DIANZUMBA, S FLACK, JM CUTLER, JA GRIMM, R STAMLER, J TI ECHOCARDIOGRAPHIC CORRELATES OF LEFT-VENTRICULAR STRUCTURE AMONG 844 MILDLY HYPERTENSIVE MEN AND WOMEN IN THE TREATMENT OF MILD HYPERTENSION STUDY (TOMHS) SO CIRCULATION LA English DT Article DE HYPERTROPHY; ECHOCARDIOGRAPHY; HYPERTENSION ID BLOOD-PRESSURE; PHYSICAL-ACTIVITY; HEART-DISEASE; CARDIAC ADAPTATION; MASS; HYPERTROPHY; POPULATION; MORTALITY; DIMENSIONS; MORBIDITY AB Background. Echocardiography provides a noninvasive means of assessing left ventricular (LV) structure and evidence of LV wall remodeling in hypertensive persons. The relation of demographic, biological, and other factors with LV structure can be assessed. Methods and Results. LV structure was assessed by M-mode echocardiograms for 511 men and 333 women with mild hypertension (average blood pressure, 140/91 mm Hg). Measurements of LV wall thicknesses and internal dimensions were made, and estimates of LV mass indexes and other derivations of structure were calculated. LV hypertrophy criteria were based on previously reported echocardiographic population studies of normal subjects. These measures were compared by age, sex, race, body mass index, systolic blood pressure, antihypertensive drug use, physical activity, alcohol intake, cigarette smoking, and urinary sodium excretion. Despite virtual absence of ECG-determined LV hypertrophy, 13% of men and 20% of women had echocardiographically determined LV hypertrophy indexed by body surface area (g/m2), and 24% of men and 45% of women had LV hypertrophy indexed by height (g/m). Black participants had slightly higher mean levels of wall thickness than nonblack participants but similar LV mass. Systolic blood pressure and urinary sodium excretion were significantly and independently associated with LV mass index and LV hypertrophy using both g/m2 and g/m. Body mass index was significantly related to LV mass index and LV hypertrophy using g/m. Smoking was significantly associated with LV mass index, i.e., using continuous measurement but not using the dichotomy for LV hypertrophy. Conclusions. This study of a large population of men and women with mild primary hypertension, largely without ECG evidence of LV hypertrophy, -showed a substantial percentage of participants with echocardiographically determined LV hypertrophy. LV mass indexes correlated positively with systolic blood pressure, body mass index, urinary sodium excretion, and smoking. C1 UNIV MINNESOTA,DEPT BIOSTAT,DIV BIOSTAT,2221 UNIV AVE,SUITE 200,MINNEAPOLIS,MN 55414. RUSH PRESBYTERIAN ST LUKES MED CTR,CARDIOL SECT,CHICAGO,IL 60612. UNIV MIAMI,DEPT EPIDEMIOL & PUBL HLTH,CORAL GABLES,FL 33124. ALLEGHENY GEN HOSP,NONINVAS LAB,PITTSBURGH,PA 15212. UNIV MINNESOTA,DIV EPIDEMIOL,MINNEAPOLIS,MN 55455. NHLBI,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD 20892. NORTHWESTERN UNIV,SCH MED,DEPT COMMUNITY HLTH & PREVENT MED,CHICAGO,IL 60611. FU NHLBI NIH HHS [5K07HL01716-05]; PHS HHS [R01-H134707] NR 53 TC 177 Z9 181 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1993 VL 87 IS 2 BP 476 EP 486 PG 11 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA KL647 UT WOS:A1993KL64700021 PM 8425295 ER PT J AU LAKATTA, EG AF LAKATTA, EG TI DEFICIENT NEUROENDOCRINE REGULATION OF THE CARDIOVASCULAR-SYSTEM WITH ADVANCING AGE IN HEALTHY HUMANS SO CIRCULATION LA English DT Editorial Material DE BETA-ADRENERGIC RECEPTOR STIMULATION; CARDIOVASCULAR SYSTEM, AGING ID BETA-ADRENERGIC-RECEPTOR; CONGESTIVE-HEART-FAILURE; NOREPINEPHRINE-INDUCED PHOSPHORYLATION; INDUCED HETEROLOGOUS DESENSITIZATION; ADENYLATE-CYCLASE ACTIVITY; PROTEIN ALPHA-SUBUNITS; RAT CARDIAC MYOCYTES; PLASMA-CATECHOLAMINES; MYOFILAMENT PROTEINS; ADENOSINE INHIBITION RP LAKATTA, EG (reprint author), NIA,GERONTOL RES CTR,CARDIOVASC SCI LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 73 TC 78 Z9 79 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1993 VL 87 IS 2 BP 631 EP 636 PG 6 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA KL647 UT WOS:A1993KL64700036 PM 8425306 ER PT J AU BILD, DE MANOLIO, TA PERKINS, LL JACOBS, DR AF BILD, DE MANOLIO, TA PERKINS, LL JACOBS, DR TI SMOKING IS ASSOCIATED WITH A GREATER AGE-RELATED INCREASE IN TOTAL CHOLESTEROL - THE CARDIA STUDY SO CIRCULATION LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1993 VL 87 IS 2 BP 687 EP 687 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA KL647 UT WOS:A1993KL64700086 ER PT J AU COOPER, LS GOLDBOURT, U FOLSOM, AR SKELTON, T WHITE, A SIERADZAN, W AF COOPER, LS GOLDBOURT, U FOLSOM, AR SKELTON, T WHITE, A SIERADZAN, W TI CARDIAC ENZYMES AND INHOSPITAL MORTALITY FROM ACUTE MYOCARDIAL-INFARCTION SO CIRCULATION LA English DT Meeting Abstract C1 NHLBI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD FEB PY 1993 VL 87 IS 2 BP 696 EP 696 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA KL647 UT WOS:A1993KL64700124 ER PT J AU LENFANT, C SHEFFER, AL AF LENFANT, C SHEFFER, AL TI INTERNATIONAL CONSENSUS REPORT ON DIAGNOSIS AND MANAGEMENT OF ASTHMA - REPLY SO CLINICAL AND EXPERIMENTAL ALLERGY LA English DT Letter C1 HARVARD UNIV,SCH MED,INT CONSENSUS REPORT,BOSTON,MA 02115. RP LENFANT, C (reprint author), NHLBI,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0954-7894 J9 CLIN EXP ALLERGY JI Clin. Exp. Allergy PD FEB PY 1993 VL 23 IS 2 BP 155 EP 155 DI 10.1111/j.1365-2222.1993.tb00313.x PG 1 WC Allergy; Immunology SC Allergy; Immunology GA KP495 UT WOS:A1993KP49500012 ER PT J AU GELMAN, R CHENG, SC KIDD, P WAXDAL, M KAGAN, J AF GELMAN, R CHENG, SC KIDD, P WAXDAL, M KAGAN, J TI ASSESSMENT OF THE EFFECTS OF INSTRUMENTATION, MONOCLONAL-ANTIBODY, AND FLUOROCHROME ON FLOW CYTOMETRIC IMMUNOPHENOTYPING - A REPORT BASED ON 2 YEARS OF THE NIAID DAIDS FLOW-CYTOMETRY QUALITY ASSESSMENT PROGRAM SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID INFECTION; AIDS; ASSURANCE; SUBSETS; ISSUES; TRIAL C1 HARVARD UNIV,SCH MED,BOSTON,MA 02115. UNIV WASHINGTON,SEATTLE,WA 98195. FAST SYST INC,GAITHERSBURG,MD 20877. NIAID,DIV AIDS,BETHESDA,MD 20892. RP GELMAN, R (reprint author), HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115, USA. FU NIAID NIH HHS [AI-95030] NR 23 TC 62 Z9 62 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD FEB PY 1993 VL 66 IS 2 BP 150 EP 162 DI 10.1006/clin.1993.1019 PG 13 WC Immunology; Pathology SC Immunology; Pathology GA KJ524 UT WOS:A1993KJ52400008 PM 7680972 ER PT J AU RABINOWITCH, BL NAM, MH LEVY, CS SMITH, MA AF RABINOWITCH, BL NAM, MH LEVY, CS SMITH, MA TI VIBRIO-PARAHAEMOLYTICUS SEPTICEMIA ASSOCIATED WITH WATER-SKIING SO CLINICAL INFECTIOUS DISEASES LA English DT Letter ID INFECTIONS C1 NIH,DEPT CRIT CARE,BETHESDA,MD 20892. RP RABINOWITCH, BL (reprint author), WASHINGTON HOSP CTR,DIV INFECT DIS,110 IRVING ST NW,WASHINGTON,DC 20010, USA. NR 6 TC 3 Z9 3 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD FEB PY 1993 VL 16 IS 2 BP 339 EP 340 PG 2 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA KK667 UT WOS:A1993KK66700036 PM 8507240 ER PT J AU MCLAREN, C ROZENCWEIG, M BELTANGADY, M NICAISE, C YARCHOAN, R DOLIN, R ALLAN, JD COOLEY, TP SEIDLIN, M SMALDONE, LF AF MCLAREN, C ROZENCWEIG, M BELTANGADY, M NICAISE, C YARCHOAN, R DOLIN, R ALLAN, JD COOLEY, TP SEIDLIN, M SMALDONE, LF TI LONGITUDINAL ANALYSIS OF RESPONSES TO ORAL DIDANOSINE THERAPY FOLLOWING ZIDOVUDINE THERAPY IN ADVANCED INFECTION WITH HUMAN-IMMUNODEFICIENCY-VIRUS SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID AIDS-RELATED COMPLEX; PLACEBO-CONTROLLED TRIAL; PHASE-I TRIAL; DOUBLE-BLIND; P24 ANTIGEN; 2',3'-DIDEOXYINOSINE DDI; REDUCED SENSITIVITY; AZIDOTHYMIDINE AZT; HIV INFECTION; TOXICITY AB The effect on immunologic and virological parameters of up to 24 weeks of therapy with didanosine at daily oral doses of less-than-or-equal-to 12.5 mg/(kg.d) was studied retrospectively in 69 patients with advanced disease due to human immunodeficiency virus-i.e., AIDS or advanced AIDS-related complex-who had previously been treated with zidovudine. Patients entered the study with a low CD4 cell count (median, 39/muL) and with evidence of an ongoing depression of bone marrow function. Didanosine therapy was associated with a significant increase in CD4 counts and a prolonged decrease in serum levels of p24 antigen relative to baseline. These changes were more pronounced in the population with baseline CD4 counts of greater-than-or-equal-to 100/muL. A beneficial effect of didanosine therapy on hematologic parameters was observed in these patients, with increases during therapy of hemoglobin levels as well as white blood cell, granulocyte, and platelet counts. These responses were maximal at weeks 16-20. Further investigations are needed to establish the clinical correlates of these observations. C1 BRISTOL MYERS SQUIBB CO,WALLINGFORD,CT. NCI,BETHESDA,MD 20892. UNIV ROCHESTER,MED CTR,ROCHESTER,NY 14642. HARVARD UNIV,SCH MED,BOSTON,MA 02115. BOSTON CITY HOSP,BOSTON,MA 02118. NYU MED CTR,NEW YORK,NY 10016. NR 35 TC 4 Z9 4 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD FEB PY 1993 VL 16 SU 1 BP S32 EP S39 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA KL251 UT WOS:A1993KL25100007 PM 8425018 ER PT J AU ANDRICH, MP CHEN, CC GALLIN, JI AF ANDRICH, MP CHEN, CC GALLIN, JI TI ABNORMAL BONE-SCINTIGRAPHY BEFORE CLINICAL SYMPTOMS IN A PATIENT WITH DEFECTIVE PHAGOCYTE FUNCTION SO CLINICAL NUCLEAR MEDICINE LA English DT Note C1 NIAID,HOST DEF LAB,BETHESDA,MD 20892. RP ANDRICH, MP (reprint author), NIAID,WARREN G MAGNUSON CLIN CTR,DEPT NUCL MED,BETHESDA,MD 20892, USA. NR 2 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0363-9762 J9 CLIN NUCL MED JI Clin. Nucl. Med. PD FEB PY 1993 VL 18 IS 2 BP 153 EP 154 DI 10.1097/00003072-199302000-00017 PG 2 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA KL748 UT WOS:A1993KL74800017 PM 8432064 ER PT J AU JASINSKI, DR SULLIVAN, JT CONE, E PRESTON, KL TESTA, MP AF JASINSKI, DR SULLIVAN, JT CONE, E PRESTON, KL TESTA, MP TI PHARMACODYNAMIC AND PHARMACOKINETIC COMPARISON OF SUBCUTANEOUSLY (SC) AND INTRAVENOUSLY (IV) GIVEN COCAINE (C) SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. NIDA,BALTIMORE,MD. NR 0 TC 3 Z9 3 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 174 EP 174 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900156 ER PT J AU MONTOYA, ID HAERTZEN, C AF MONTOYA, ID HAERTZEN, C TI THERAPEUTIC EFFECT OF NON-TREATMENT DRUG-ABUSE RESIDENTIAL TRIALS SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIDA,ARC,BALTIMORE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 174 EP 174 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900154 ER PT J AU JOHNSON, RE RISHERFLOWERS, DL ALIM, TN MASTROPAOLO, J VOCCI, FJ DEUTSCH, SI AF JOHNSON, RE RISHERFLOWERS, DL ALIM, TN MASTROPAOLO, J VOCCI, FJ DEUTSCH, SI TI BLOCKADE OF INTRAVENOUS HYDROMORPHONE (H) BY METHADONE (M) AND BUPRENORPHINE (B) SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. VET ADM MED CTR,WASHINGTON,DC. NIDA,DIV MEDICAT DEV,ROCKVILLE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 175 EP 175 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900160 ER PT J AU SULLIVAN, JT CONE, E JASINSKI, DR AF SULLIVAN, JT CONE, E JASINSKI, DR TI IV NALOXONE RAISES SERUM MORPHINE LEVELS SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. NIDA,BALTIMORE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 175 EP 175 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900161 ER PT J AU GEORGE, DT PHILLIPS, M LINNOILA, M AF GEORGE, DT PHILLIPS, M LINNOILA, M TI EFFECTS OF PROCAINE ON SUBJECTS WITH AND WITHOUT PANIC DISORDER SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIAAA,BETHESDA,MD. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 179 EP 179 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900175 ER PT J AU KELLY, JF SONCRANT, TT MIDHA, KK HUBBARD, JW MCKAY, G RAPOPORT, SI AF KELLY, JF SONCRANT, TT MIDHA, KK HUBBARD, JW MCKAY, G RAPOPORT, SI TI PHARMACOKINETICS OF INTRAVENOUS HALOPERIDOL IN HEALTHY-YOUNG AND OLD SUBJECTS SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIA,NEUROSCI LAB,BALTIMORE,MD 21224. UNIV SASKATCHEWAN,COLL PHARM,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 192 EP 192 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900229 ER PT J AU KORRAPATI, MR LOI, CM SORKIN, JD VESELL, ES ANDRES, R VESTAL, RE AF KORRAPATI, MR LOI, CM SORKIN, JD VESELL, ES ANDRES, R VESTAL, RE TI ACETYLATOR PHENOTYPE IN RELATION TO AGE AND GENDER IN THE BALTIMORE LONGITUDINAL-STUDY OF AGING SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 VET ADM MED CTR,CLIN PHARMACOL & GERONTOL RES UNIT,BOISE,ID. IDAHO STATE UNIV,COLL PHARM,POCATELLO,ID 83209. NIA,GERONTOL RES CTR,BALTIMORE,MD 21224. PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,HERSHEY,PA 17033. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 192 EP 192 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900228 ER PT J AU SHOAF, SE HIGLEY, JD LINNOILA, M AF SHOAF, SE HIGLEY, JD LINNOILA, M TI PHARMACOKINETICS OF IPSAPIRONE IN RHESUS-MONKEYS FOLLOWING IV AND ORAL-ADMINISTRATION SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NIAAA,DICBR,LCS,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 212 EP 212 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900307 ER PT J AU FIGG, WD COOPER, MR VITETTA, E MYERS, CE THIBAULT, A SAUSVILLE, EA AF FIGG, WD COOPER, MR VITETTA, E MYERS, CE THIBAULT, A SAUSVILLE, EA TI IMMUNOTOXIN IGG-RFB4-SMPT-DGA (IMX) PHARMACOKINETICS (PKS) IN REFRACTORY CD22-POSITIVE B-CELL LYMPHOMA SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 NCI,CLIN PHARMACOL BRANCH,BETHESDA,MD 20892. UNIV TEXAS,SW MED CTR,DEPT MICROBIOL,DALLAS,TX 75230. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1993 VL 53 IS 2 BP 232 EP 232 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA KP269 UT WOS:A1993KP26900385 ER PT J AU STAMATOYANNOPOULOS, JA LOWREY, CH NIENHUIS, AW AF STAMATOYANNOPOULOS, JA LOWREY, CH NIENHUIS, AW TI SELECTIVE MUTATION OF AP-1/NF-E2 AND GATA-1 SITES IN 5' DNASE-I HYPERSENSITIVE SITE-IV OF THE BETA-GLOBIN LOCUS-CONTROL REGION HAVE DIFFERENTIAL-EFFECTS ON HYPERSENSITIVE SITE FORMATION IN MEL CELLS SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 UNIV WASHINGTON,SCH MED,SEATTLE,WA 98195. NHLBI,CLIN HEMATOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1993 VL 41 IS 1 BP A17 EP A17 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA KH410 UT WOS:A1993KH41000089 ER PT J AU STEVENSON, DK VREMAN, HJ OH, W FANAROFF, AA LEMONS, JA WRIGHT, E WRIGHT, L AF STEVENSON, DK VREMAN, HJ OH, W FANAROFF, AA LEMONS, JA WRIGHT, E WRIGHT, L TI BILIRUBIN PRODUCTION IN HEALTHY TERM INFANTS WITHOUT HEMOLYSIS SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 STANFORD UNIV,MED CTR,SCH MED,DEPT PEDIAT,STANFORD,CA 94305. NICHHD,NEONATAL RES NETWORK,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1993 VL 41 IS 1 BP A72 EP A72 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA KH410 UT WOS:A1993KH41000391 ER PT J AU VREMAN, HJ STEVENSON, DK OH, W FANAROFF, AA LEMONS, JA WRIGHT, E WRIGHT, L AF VREMAN, HJ STEVENSON, DK OH, W FANAROFF, AA LEMONS, JA WRIGHT, E WRIGHT, L TI DETERMINATION OF END-TIDAL CARBON-MONOXIDE AND BILIRUBIN PRODUCTION WITH A SEMIPORTABLE BREATH ANALYZER SO CLINICAL RESEARCH LA English DT Meeting Abstract C1 STANFORD UNIV,MED CTR,SCH MED,DEPT PEDIAT,STANFORD,CA 94305. NICHHD,NEONATAL RES NETWORK,BETHESDA,MD. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0009-9279 J9 CLIN RES JI Clin. Res. PD FEB PY 1993 VL 41 IS 1 BP A23 EP A23 PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA KH410 UT WOS:A1993KH41000118 ER PT J AU ALBRO, PW CORBETT, JT SCHROEDER, JL AF ALBRO, PW CORBETT, JT SCHROEDER, JL TI THE METABOLISM OF DI-(2-ETHYLHEXYL) PHTHALATE IN THE EARTHWORM LUMBRICUS-TERRESTRIS SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY LA English DT Article ID ESTERS; URINE AB 1. Earthworms can hydrolyze di-(2-ethylhexyl) phthalate (DEHP) to mono-2-ethylhexyl phthalate (MEHP) and phthalic acid (PA). 2. They apparently cannot produce the side-chain-oxidized derivatives of MEHP that constitute the major DEHP metabolites in higher animals. 3. With the assistance of intestinal bacterial Pseudomonas, the worm-derived PA is degraded through protocatechuic and beta-carboxymuconic acids to CO2. 4. There is an indication of a second pathway for degradation of PA leading through benzoic acid. RP ALBRO, PW (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 29 TC 9 Z9 9 U1 4 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0742-8413 J9 COMP BIOCHEM PHYS C JI Comp. Biochem. Physiol. C-Pharmacol. Toxicol. Endocrinol. PD FEB PY 1993 VL 104 IS 2 BP 335 EP 344 DI 10.1016/0742-8413(93)90045-M PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology GA KV598 UT WOS:A1993KV59800021 PM 8098688 ER PT J AU LEMKIN, PF AF LEMKIN, PF TI XCONF - A NETWORK-BASED IMAGE CONFERENCING SYSTEM SO COMPUTERS AND BIOMEDICAL RESEARCH LA English DT Article ID COMMUNICATION RP LEMKIN, PF (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DCBDC,MATH BIOL LAB,IMAGE PROC SECT,BLDG 469,ROOM 150B,FREDERICK,MD 21702, USA. NR 32 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0010-4809 J9 COMPUT BIOMED RES JI Comput. Biomed. Res. PD FEB PY 1993 VL 26 IS 1 BP 1 EP 27 DI 10.1006/cbmr.1993.1001 PG 27 WC Computer Science, Interdisciplinary Applications; Medical Informatics SC Computer Science; Medical Informatics GA KK791 UT WOS:A1993KK79100001 PM 8444025 ER PT J AU KRASNEGOR, NA AF KRASNEGOR, NA TI BIOLOGICAL RISK-FACTORS FOR PSYCHOSOCIAL DISORDERS - RUTTER,M, CASAER,P SO CONTEMPORARY PSYCHOLOGY LA English DT Book Review RP KRASNEGOR, NA (reprint author), NICHHD,HUMAN LEARNING & BEHAV BRANCH,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0010-7549 J9 CONTEMP PSYCHOL JI Comtemp. Psychol. PD FEB PY 1993 VL 38 IS 2 BP 167 EP 168 PG 2 WC Psychology, Multidisciplinary SC Psychology GA KL522 UT WOS:A1993KL52200034 ER PT J AU SIEBERT, C CLARK, CM AF SIEBERT, C CLARK, CM TI OPERATIONAL AND POLICY CONSIDERATIONS OF DATA MONITORING IN CLINICAL-TRIALS - THE DIABETES CONTROL AND COMPLICATIONS TRIAL EXPERIENCE SO CONTROLLED CLINICAL TRIALS LA English DT Article DE TREATMENT EFFECTS MONITORING; ORGANIZATION; METHODS OR ISSUES; DOCUMENTATION ID INTERIM ANALYSIS AB Many clinical trials incorporate a system for monitoring emerging data that utilizes a committee composed of individuals who are independent of the investigators conducting the study. Although this is a common practice, there is a paucity of publications examining the operating methods of these groups. This paper describes the composition, functions, and procedures of the Data, Safety and Quality Review Group (DSQ) of the Diabetes Control and Complications Trial (DCCT). The DSQ is not masked to emerging data and the voting membership is made up of individuals with a wide diversity of expertise that reflects the needs of the trial. There are also nonvoting ex-officio members who represent other components of the study organization. In addition to data monitoring, the DSQ provides external review of coordinating center operations. A distinctive aspect of the DCCT's DSQ is the creation of a free-standing operations manual for the DSQ's use during the trial and the involvement of the study investigators in developing certain sections of this document. Utilizing a process that allowed participation of the investigators was considered critical to achieving a mutual understanding regarding the planned uses and interpretations of the study data prior to the study's completion so as to minimize the chances of major disagreements regarding the conclusions drawn. Equally important, the existence of such a manual provides documentable reassurance to all interested parties that both scientific integrity and patient safety are being closely watched and gives the study investigators confidence that the results of the study will be scientifically credible and clinically important. C1 INDIANA UNIV, SAFETY & QUAL REVIEW GRP, BLOOMINGTON, IN 47401 USA. RP SIEBERT, C (reprint author), NIDDK, DEMD, 628 WESTWOOD BLDG, BETHESDA, MD 20892 USA. NR 28 TC 8 Z9 8 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-2456 EI 1879-050X J9 CONTROL CLIN TRIALS JI Controlled Clin. Trials PD FEB PY 1993 VL 14 IS 1 BP 30 EP 44 DI 10.1016/0197-2456(93)90048-I PG 15 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA KN032 UT WOS:A1993KN03200004 PM 8440093 ER PT J AU CURTI, BD AF CURTI, BD TI PHYSICAL BARRIERS TO DRUG DELIVERY IN TUMORS SO CRITICAL REVIEWS IN ONCOLOGY/HEMATOLOGY LA English DT Review ID HUMAN-ENDOTHELIAL-CELLS; MAGNETIC-RESONANCE SPECTROSCOPY; VASCULAR-PERMEABILITY FACTOR; INTERSTITIAL FLUID PRESSURE; SOLID TUMORS; BLOOD-FLOW; MONOCLONAL-ANTIBODIES; NEOPLASTIC TISSUES; MAMMARY-CARCINOMA; MACROMOLECULES C1 GEORGETOWN UNIV,WASHINGTON,DC 20057. RP CURTI, BD (reprint author), NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,FREDERICK,MD 21701, USA. NR 99 TC 7 Z9 7 U1 0 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 1040-8428 J9 CRIT REV ONCOL HEMAT JI Crit. Rev. Oncol./Hematol. PD FEB PY 1993 VL 14 IS 1 BP 29 EP 39 DI 10.1016/1040-8428(93)90004-N PG 11 WC Oncology; Hematology SC Oncology; Hematology GA LD883 UT WOS:A1993LD88300003 PM 8373539 ER PT J AU BURNS, CM ASHWELL, JD AF BURNS, CM ASHWELL, JD TI ZAPPING THE T-CELL RECEPTOR SO CURRENT BIOLOGY LA English DT Article ID PROTEIN-TYROSINE KINASE; ANTIGEN RECEPTOR; ZETA-CHAIN; SIGNAL TRANSDUCTION; MICE LACKING; ASSOCIATION; ACTIVATION; P56(LCK); CLONING; ZAP-70 C1 NIH,BIOL RESPONSE MODIFIERS PROGRAM,IMMUNE CELL BIOL LAB,BETHESDA,MD 20892. NR 16 TC 5 Z9 5 U1 0 U2 0 PU CURRENT BIOLOGY LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD FEB PY 1993 VL 3 IS 2 BP 97 EP 99 DI 10.1016/0960-9822(93)90164-J PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA LL954 UT WOS:A1993LL95400007 PM 15335803 ER PT J AU ROBERGE, FG XU, DS CHAN, CC DESMET, MD NUSSENBLATT, RB CHEN, HF AF ROBERGE, FG XU, DS CHAN, CC DESMET, MD NUSSENBLATT, RB CHEN, HF TI TREATMENT OF AUTOIMMUNE UVEORETINITIS IN THE RAT WITH RAPAMYCIN, AN INHIBITOR OF LYMPHOCYTE GROWTH-FACTOR SIGNAL TRANSDUCTION SO CURRENT EYE RESEARCH LA English DT Article ID T-CELL ACTIVATION; CYCLOSPORIN-A; S-ANTIGEN; LEWIS RATS; UVEITIS; FK-506; DISEASE; FK506; RETINITIS; SUPPRESSION AB Rapamycin (RAPA) is a macrolide antibiotic with unique immunosuppressive properties. RAPA inhibits T-cell function by interfering with IL-2 and IL-4 signal transduction. It does not prevent IL-2 production or IL-2R expression. The efficacy of RAPA in the treatment of autoimmune diseases was evaluated using the experimental autoimmune uveoretinitis (EAU) model. EAU was actively induced in Lewis rats by immunization with S-antigen in Hunter's adjuvant. RAPA and control vehicle were administered by continuous intravenous infusion over a 14 day period by miniosmotic pump. RAPA treatment initiated on the day of immunization or 7 days later was found to efficiently inhibit EAU induction. The minimal effective dose was 0.1 mg/kg/d. EAU inhibition was correlated with reduced number of cells in the immunization site draining lymph nodes, as well as with a shift and lowering of the peak of the lymphocyte proliferative response curve. The anti-S-antigen antibody response was delayed by 3 days under RAPA treatment and the serum levels lowered in a dose dependent manner. An initial body weight loss was observed during the first week of drug administration, but there was a normal weight gain afterward. C1 NOTRE DAME HOSP,EXPTL SURG LAB,MONTREAL,PQ,CANADA. RP ROBERGE, FG (reprint author), NEI,IMMUNOL LAB,BLDG 10,ROOM 10N 202,BETHESDA,MD 20892, USA. OI de Smet, Marc/0000-0002-9217-5603 NR 42 TC 37 Z9 38 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0271-3683 J9 CURR EYE RES JI Curr. Eye Res. PD FEB PY 1993 VL 12 IS 2 BP 197 EP 203 DI 10.3109/02713689308999487 PG 7 WC Ophthalmology SC Ophthalmology GA KP314 UT WOS:A1993KP31400011 PM 8449029 ER PT J AU WOLF, SS HYDE, TM WEINBERGER, DR AF WOLF, SS HYDE, TM WEINBERGER, DR TI NEUROBIOLOGY OF SCHIZOPHRENIA SO CURRENT OPINION IN NEUROLOGY AND NEUROSURGERY LA English DT Article ID POSITRON EMISSION TOMOGRAPHY; ENTORHINAL REGION; RECEPTOR GENE; BRAIN; ABNORMALITIES; CHROMOSOME-5; DISTURBANCES; EXPRESSION; PSYCHOSIS AB Schizophrenia is the most prevalent of the major psychoses, but the underlying neurobiology of this debilitating disorder remains mysterious. Recent developments in molecular biology, neuroanatomic pathology, neurochemistry, and functional imaging suggest that a number of factors converge to produce schizophrenia. Specifically, an early neurodevelopmental ''lesion,'' possibly within the mesial temporal lobe, may contribute to later temporolimbic-prefrontal dysfunction as the nervous system matures. Genetic factors appear to facilitate liability to schizophrenia, and dopaminergic and possibly other neurotransmitter systems may mediate clinical expression of the illness through newly recognized receptor subtypes. RP WOLF, SS (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,INTRAMURAL RES PROGRAM,WASHINGTON,DC 20032, USA. NR 40 TC 21 Z9 21 U1 1 U2 1 PU CURRENT SCIENCE LTD PI LONDON PA 34-42 CLEVELAND STREET, LONDON, ENGLAND W1P 6LB SN 0951-7383 J9 CURR OPIN NEUROL NEU PD FEB PY 1993 VL 6 IS 1 BP 86 EP 92 PG 7 WC Neurosciences SC Neurosciences & Neurology GA KJ650 UT WOS:A1993KJ65000016 PM 8428072 ER PT J AU SUMMERS, RG MORRILL, JB LEITH, A MARKO, M PISTON, DW STONEBRAKER, AT AF SUMMERS, RG MORRILL, JB LEITH, A MARKO, M PISTON, DW STONEBRAKER, AT TI A STEREOMETRIC ANALYSIS OF KARYOKINESIS, CYTOKINESIS AND CELL ARRANGEMENTS DURING AND FOLLOWING 4TH CLEAVAGE PERIOD IN THE SEA-URCHIN, LYTECHINUS-VARIEGATUS SO DEVELOPMENT GROWTH & DIFFERENTIATION LA English DT Article DE SEA URCHIN EMBRYO; CELL DIVISION PATTERNS; STEREO IMAGING; 3-D RECONSTRUCTION ID UNEQUAL CLEAVAGE; PARACENTROTUS-LIVIDUS; EMBRYOS; BLASTOMERES; MICROSCOPY; DIVISION; RECONSTRUCTION; GASTRULATION; SURFACE C1 UNIV S FLORIDA,NEW COLL,DIV NAT SCI,SARASOTA,FL 34243. NIH,BIOL MICROSCOPY & IMAGE RECONSTRUCT RESOURCE,WADSWORTH CTR LABS & RES,ALBANY,NY 12201. CORNELL UNIV,SCH APPL & ENGN PHYS,ITHACA,NY 14853. RP SUMMERS, RG (reprint author), SUNY BUFFALO,SCH MED & BIOMED SCI,DEPT ANAT & CELL BIOL,BUFFALO,NY 14214, USA. NR 50 TC 16 Z9 16 U1 0 U2 1 PU BLACKWELL SCIENCE PI CARLTON PA 54 UNIVERSITY ST, P O BOX 378, CARLTON VICTORIA 3053, AUSTRALIA SN 0012-1592 J9 DEV GROWTH DIFFER JI Dev. Growth Diff. PD FEB PY 1993 VL 35 IS 1 BP 41 EP 57 PG 17 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA KT826 UT WOS:A1993KT82600005 ER PT J AU LI, X ZELENKA, PS PIATIGORSKY, J AF LI, X ZELENKA, PS PIATIGORSKY, J TI DIFFERENTIAL EXPRESSION OF THE 2 DELTA-CRYSTALLIN GENES IN LENS AND NONLENS TISSUES - SHIFT FAVORING DELTA-2 EXPRESSION FROM EMBRYONIC TO ADULT CHICKENS SO DEVELOPMENTAL DYNAMICS LA English DT Article DE LENS; ARGININOSUCCINATE LYASE (ASL) DELTA-CRYSTALLIN; CHICKEN DEVELOPMENT; GENE EXPRESSION; MESSENGER RNA; POLYMERASE CHAIN REACTION; ISOENZYME ID ARGININOSUCCINATE LYASE GENE; POLYMERASE CHAIN-REACTION; NUCLEOTIDE-SEQUENCE; STRUCTURAL PROTEIN; NONLENS TISSUES; ENZYME; CELLS; RNA; DELTA-2-CRYSTALLIN; AMPLIFICATION AB Chicken argininosuccinate lyase (ASL)/delta-crystallin, a lens enzyme-crystallin, is encoded in two linked genes (delta1 and delta2); only the delta2 polypeptide contains ASL activity. Here we have quantified delta1- and delta2-crystallin mRNA in the lens, cornea, neural retina, heart, and brain at different stages of embryonic development and in 1-wk-old and 1-yr-old chickens by the polymerase chain reaction using internal delta1 and delta2 RNA standards. The delta1/delta2 mRNA ratio differed for every tissue and was regulated during development. In the embryo there was more delta1 than delta2 mRNA in the lens (50-100 times), cornea (3-4 times), and neural retina (2-20 times), about equal amounts of delta1 and delta2 mRNA in the heart, and more delta2 mRNA in the brain (15 times). Delta1-crystallin mRNA differentially decreased in every tissue after hatching; by contrast, the delta2 mRNA remained about the same except for the lens, where it decreased 50-fold between 1 wk and 1 yr after hatching. In the 1-yr-old chicken, the delta2/delta1 mRNA ratios were 7 in the lens, 175 in the cornea, 22 in the neural retina, 107 in the heart, and 136 in the brain, indicating that delta2-crystallin is strongly favored in all adult tissues of the chicken. The excess of delta1 to delta2 mRNA in the embryonic lens, cornea, and neural retina is intriguing, and suggests some connection with developing transparent eye tissues. Finally, we raise the possibility that expression of both delta-crystallin genes may create tetrameric ASL isoenzymes (perhaps with different specific activities). The unexpected predominance of delta2 mRNA in the 1-yr-old lens suggests that both the enzymatic and refractive functions of ASL/delta-crystallin are operative and spatially separated, with the enzymatic role present in the cortical fibers and the refractive role in the center of the lens. (C) 1993 Wiley-Liss, Inc. C1 NEI, MOLEC & DEV BIOL LAB,BLDG 6,RM 201, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 57 TC 9 Z9 9 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1058-8388 EI 1097-0177 J9 DEV DYNAM JI Dev. Dyn. PD FEB PY 1993 VL 196 IS 2 BP 114 EP 123 PG 10 WC Anatomy & Morphology; Developmental Biology SC Anatomy & Morphology; Developmental Biology GA LL239 UT WOS:A1993LL23900004 PM 8364221 ER PT J AU STAMLER, J VACCARO, O NEATON, JD WENTWORTH, D AF STAMLER, J VACCARO, O NEATON, JD WENTWORTH, D TI DIABETES, OTHER RISK-FACTORS, AND 12-YR CARDIOVASCULAR MORTALITY FOR MEN SCREENED IN THE MULTIPLE RISK FACTOR INTERVENTION TRIAL SO DIABETES CARE LA English DT Article ID CORONARY HEART-DISEASE; GLYCOSYLATION END-PRODUCTS; UNITED-STATES POPULATION; GLUCOSE-TOLERANCE; VASCULAR-DISEASE; MELLITUS; INSULIN; COMPLICATIONS; COHORT; NIDDM AB OBJECTIVE - To assess predictors of CVD mortality among men with and without diabetes and to assess the independent effect of diabetes on the risk of CVD death. RESEARCH DESIGN AND METHODS - Participants in this cohort study were screened from 1973 to 1975; vital status has been ascertained over an average of 12 yr of follow-up (range 11-13 yr). Participants were 347,978 men aged 35-57 yr, screened in 20 centers for MRFIT. The outcome measure was CVD mortality. RESULTS - Among 5163 men who reported taking medication for diabetes, 1092 deaths (603 CVD deaths) occurred in an average of 12 yr of follow-up. Among 342,815 men not taking medication for diabetes, 20,867 deaths were identified, 8965 ascribed to CVD. Absolute risk of CVD death was much higher for diabetic than nondiabetic men of every age stratum, ethnic background, and risk factor level-overall three times higher, with adjustment for age, race, income, serum cholesterol level, sBP, and reported number of cigarettes/day (P < 0.000 1). For men both with and without diabetes, serum cholesterol level, sBP, and cigarette smoking were significant predictors of CVD mortality. For diabetic men with higher values for each risk factor and their combinations, absolute risk of CVD death increased more steeply than for nondiabetic men, so that absolute excess risk for diabetic men was progressively greater than for nondiabetic men with higher risk factor levels. CONCLUSIONS - These findings emphasize the importance of rigorous sustained intervention in people with diabetes to control blood pressure, lower serum cholesterol, and abolish cigarette smoking, and the importance of considering nutritional-hygienic approaches on a mass scale to prevent diabetes. C1 UNIV NAPLES,FAC MED 2,DEPT INTERNAL MED & METAB DIS,I-80138 NAPLES,ITALY. UNIV MINNESOTA,SCH PUBL HLTH,DIV BIOSTAT,MINNEAPOLIS,MN 55455. NHLBI,BETHESDA,MD 20892. RP STAMLER, J (reprint author), NORTHWESTERN UNIV,SCH MED,DEPT PREVENT MED,680 N LAKE SHORE DR,SUITE 1102,CHICAGO,IL 60611, USA. FU NHLBI NIH HHS [1-RO1-HL-28715] NR 67 TC 2563 Z9 2676 U1 8 U2 58 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0149-5992 J9 DIABETES CARE JI Diabetes Care PD FEB PY 1993 VL 16 IS 2 BP 434 EP 444 DI 10.2337/diacare.16.2.434 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KK682 UT WOS:A1993KK68200004 PM 8432214 ER PT J AU CANNON, RO BENJAMIN, SB AF CANNON, RO BENJAMIN, SB TI CHEST PAIN AS A CONSEQUENCE OF ABNORMAL VISCERAL NOCICEPTION SO DIGESTIVE DISEASES AND SCIENCES LA English DT Editorial Material ID NORMAL CORONARY-ARTERIES; FLOW RESERVE; MICROVASCULAR ANGINA; PSYCHIATRIC-ILLNESS; ESOPHAGEAL MOTILITY; PANIC DISORDER; FOLLOW-UP; ARTERIOGRAMS; PECTORIS; EXERCISE C1 GEORGETOWN UNIV HOSP,DIV GASTROENTEROL,GASTROENTEROL SECT,3800 RESERVOIR RD NW,ROOM 2118,WASHINGTON,DC 20007. NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NR 27 TC 32 Z9 32 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0163-2116 J9 DIGEST DIS SCI JI Dig. Dis. Sci. PD FEB PY 1993 VL 38 IS 2 BP 193 EP 196 DI 10.1007/BF01307534 PG 4 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA KL393 UT WOS:A1993KL39300001 PM 8425431 ER PT J AU METZ, DC PISEGNA, JR RINGHAM, GL FEIGENBAUM, K KOVIACK, PD MATON, PN GARDNER, JD JENSEN, RT AF METZ, DC PISEGNA, JR RINGHAM, GL FEIGENBAUM, K KOVIACK, PD MATON, PN GARDNER, JD JENSEN, RT TI PROSPECTIVE-STUDY OF EFFICACY AND SAFETY OF LANSOPRAZOLE IN ZOLLINGER-ELLISON SYNDROME SO DIGESTIVE DISEASES AND SCIENCES LA English DT Article DE GASTRINOMA; ISLET CELL TUMOR; ACID-PEPTIC DISEASE; H+,K+-ATPASE INHIBITOR ID GASTRIC-ACID SECRETION; UNITED-STATES EXPERIENCE; INTRAVENOUS OMEPRAZOLE; HYPERSECRETORY STATES; SYNDROME ZES; CIMETIDINE; RANITIDINE; FAMOTIDINE; MANAGEMENT; THERAPY AB Lansoprazole, a new substituted benzimidazole H+,K+-ATPase inhibitor, profoundly inhibits gastric acid secretion and has potential use in the management of diseases such as Zollinger-Ellison syndrome (ZES). In the present study we evaluated the efficacy and safety of lansoprazole in controlling acid hypersecretion in 20 patients with ZES. The starting dose was 60 mg once daily. Control of acid hypersecretion was defined as the dose required to reduce acid secretion to <10 meq/hr in the last hour before the next dose. Doses were adjusted upwards until effective control was achieved. Patients not controlled with 120 mg once daily were placed on twice daily lansoprazole. Most patients (90%) required lansoprazole once daily. During long-term follow-up (mean 18.5 months), 25% of patients required upward dose adjustments and 25% of patients required twice daily lansoprazole. Following cessation of therapy, the mean time for gastric acid output to reach half basal acid output was 39.1 hr. Lansoprazole was well-tolerated without side effects. Clinical chemistry and hematological studies were unchanged, and no gastric carcinoids developed. These results demonstrate that lansoprazole is a safe and effective inhibitor of gastric acid hypersecretion in patients with Zollinger-Ellison syndrome. Because it has a long duration of action, lansoprazole can be used to control gastric acid hypersecretion in most patients with Zollinger-Ellison syndrome using a once daily dosing schedule. C1 NIDDKD,DIGEST DIS BRANCH,BLDG 10,ROOM 9C-103,BETHESDA,MD 20892. TAP PHARMACEUT INC,DEERFIELD,IL. NR 61 TC 45 Z9 45 U1 0 U2 1 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0163-2116 J9 DIGEST DIS SCI JI Dig. Dis. Sci. PD FEB PY 1993 VL 38 IS 2 BP 245 EP 256 DI 10.1007/BF01307541 PG 12 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA KL393 UT WOS:A1993KL39300008 PM 8425437 ER PT J AU PERSICO, AM OHARA, BF FARMER, S GYSIN, R FLANAGAN, SD UHL, GR AF PERSICO, AM OHARA, BF FARMER, S GYSIN, R FLANAGAN, SD UHL, GR TI DOPAMINE D2-RECEPTOR GENE TAQ-I-A LOCUS MAP INCLUDING A4 VARIANT - RELEVANCE FOR ALCOHOLISM AND DRUG-ABUSE SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE DOPAMINE D2-RECEPTOR GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; POLYMERASE CHAIN REACTION; ALCOHOLISM; DRUG ABUSE ID RECEPTOR GENE; ALLELIC ASSOCIATION; INHERITANCE; LINKAGE AB The D2 dopamine receptor gene (DRD2) displays Taq I restriction fragment length polymorphisms (RFLPs) at two different loci, termed A and B. One of the three different Taq I A 'alleles' described at this site, the Al allele (size = 6.6 kb), has been found to be associated with alcoholism and with drug abuse in the majority of studies reported to date. A complete map of the Taq I A RFLP site has been constructed, through hybridization with different fragments of the 3' flanking region and polymerase chain reaction (PCR). When screening 432 unrelated individuals to establish possible Al allelic association with drug abuse or dependence, we have encountered a novel Taq I A RFLP, which we have named 'A4' (size = 8.6 kb). This sequence variant displays a frequency of approximately 1% in our sample and shows a Mendelizing genetic pattern in an Italian nuclear family. Primers suitable for detecting A4 using PCR have been designed. The A4, but not the A3 'allele', displays substantial overlap with the Al. In particular, A2 and A3 share the presence of a Taq I restriction site, whose absence in Al and A4 is apparently associated with substance abuse vulnerability. Therefore, in association studies it is proper to contrast individuals displaying Al and A4 RFLP patterns, with individuals displaying A2 and A3 RFLPs. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205. CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV NEUROSCI,DUARTE,CA 91010. RP PERSICO, AM (reprint author), NIDA,ADDICT RES CTR,POB 5180,BALTIMORE,MD 21224, USA. FU NIMH NIH HHS [MH45908] NR 25 TC 8 Z9 8 U1 1 U2 2 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD FEB PY 1993 VL 31 IS 3 BP 229 EP 234 DI 10.1016/0376-8716(93)90005-B PG 6 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA KT311 UT WOS:A1993KT31100005 PM 8096458 ER PT J AU ODELEYE, OE WATSON, RR ESKELSON, CD EARNEST, D AF ODELEYE, OE WATSON, RR ESKELSON, CD EARNEST, D TI ENHANCEMENT OF COCAINE-INDUCED HEPATOTOXICITY BY ETHANOL SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE COCAINE; ALCOHOL; HEPATOTOXICITY; FREE RADICALS; LIPID PEROXIDATION ID LIPID-PEROXIDATION PRODUCTS; ALCOHOLIC LIVER-DISEASE; VITAMIN-E; RATS; CONSUMPTION; HEPATOCYTES; NORCOCAINE; CYTOCHROME-P-450; SUPPLEMENTATION; POTENTIATION AB The contribution of moderate ethanol consumption on cocaine induced hepatotoxicity and the role lipid peroxidation plays as a possible mechanism of such increased hepatotoxicity were evaluated. Male C57BL/6 mice were injected interperitoneally (i.p.) with increasing doses of cocaine, from 10 to 50 mg/kg body weight daily and simultaneously fed a liquid diet containing 28% of the calories as ethanol for 5 or 9 weeks. Control mice received saline (i.p.) and an isocaloric carbohydrate diet. Lipid fluorescence and conjugated dienes of extracted lipids and amounts of malondialdehyde (MDA) were evaluated as indices of lipoperoxidation. In addition, serum alanine aminotransferase and aspartate transaminase were measured as indicators of liver injury and cellular death. After 9 weeks, ethanol consumption during cocaine treatment increased hepatic lipid fluorescence, conjugated dienes and MDA about twofold over mice treated with cocaine alone. Similarly, serum transaminases were 2.8 - 6-fold greater in mice consuming alcohol and treated with cocaine than in mice treated with cocaine only. Histological examination of livers from mice fed ethanol during treatment with cocaine exhibited increased hepatic injuries and necrosis. The data suggest that ethanol exacerbates cocaine-induced hepatotoxicity via increases in free radical activity and hepatic lipid per oxidation. C1 UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT FAMILY & COMMUNITY MED,TUCSON,AZ 85724. UNIV ARIZONA,ARIZONA HLTH SCI CTR,NIAAA,SPECIALIZED ALCOHOL RES CTR,TUCSON,AZ 85724. UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT SURG,TUCSON,AZ 85724. UNIV ARIZONA,ARIZONA HLTH SCI CTR,DEPT INTERNAL MED,TUCSON,AZ 85724. FU NIAAA NIH HHS [AA-0837]; NIDA NIH HHS [DA-04827] NR 51 TC 21 Z9 21 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD FEB PY 1993 VL 31 IS 3 BP 253 EP 263 DI 10.1016/0376-8716(93)90008-E PG 11 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA KT311 UT WOS:A1993KT31100008 PM 8462414 ER PT J AU SCHILD, C CLARET, FX WAHLI, W WOLFFE, AP AF SCHILD, C CLARET, FX WAHLI, W WOLFFE, AP TI A NUCLEOSOME-DEPENDENT STATIC LOOP POTENTIATES ESTROGEN-REGULATED TRANSCRIPTION FROM THE XENOPUS VITELLOGENIN-B1 PROMOTER INVITRO SO EMBO JOURNAL LA English DT Article DE ENHANCER; ESTROGEN; NF1; NUCLEOSOME; RECEPTOR; TRANSCRIPTION ID LIVER NUCLEAR EXTRACTS; TUMOR VIRUS PROMOTER; RNA POLYMERASE-II; 5S RNA; CORE PARTICLE; DNA; GENE; CHROMATIN; RECEPTOR; EXPRESSION AB We describe the transcriptional potentiation in estrogen responsive transcription extracts of the Xenopus vitellogenin B1 gene promoter through the formation of a positioned nucleosome. Nuclease digestion and hydroxyl radical cleavage indicate that strong, DNA sequence-directed positioning of a nucleosome occurs between -300 and -140 relative to the start site of transcription. Deletion of this DNA sequence abolishes the potentiation of transcription due to nucleosome assembly. The wrapping of DNA around the histone core of the nucleosome positioned between -300 and -140 creates a static loop in which distal estrogen receptor binding sites are brought close to proximal promoter elements. This might facilitate interactions between the trans-acting factors themselves and/or RNA polymerase. Such a nucleosome provides an example of how chromatin structure might have a positive effect on the transcription process. C1 NICHHD,MOLEC EMBRYOL LAB,BLDG 6,ROOM B1A13,BETHESDA,MD 20892. UNIV LAUSANNE,INST BIOL ANIM,CH-1015 LAUSANNE,SWITZERLAND. RI Wahli, Walter/B-1398-2009; Schild-Poulter, Caroline/K-4476-2013 OI Wahli, Walter/0000-0002-5966-9089; NR 59 TC 196 Z9 198 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD FEB PY 1993 VL 12 IS 2 BP 423 EP 433 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KL717 UT WOS:A1993KL71700006 PM 8440235 ER PT J AU PAOLINI, R KINET, JP AF PAOLINI, R KINET, JP TI CELL-SURFACE CONTROL OF THE MULTIUBIQUITINATION AND DEUBIQUITINATION OF HIGH-AFFINITY IMMUNOGLOBULIN-E RECEPTORS SO EMBO JOURNAL LA English DT Article DE DEUBIQUITINATION; IGE RECEPTOR; MULTIUBIQUITINATION; SIGNALING; TRANSMEMBRANE PROTEIN ID NATURAL-KILLER-CELLS; FC-GAMMA RECEPTOR; T-CELL; TYROSINE PHOSPHORYLATION; SIGNAL TRANSDUCTION; ZETA-CHAIN; PROTEIN; FAMILY; IGE; ACTIVATION AB Multiubiquitination of proteins is a critical step leading to selective degradation for many polypeptides. Therefore, activation-induced multiubiquitination of cell surface receptors, such as the platelet-derived growth factor (PDGF) receptor and the T cell antigen (TCR) receptor, may correspond to a degradation pathway for ligand-receptor complexes. Here we show that the antigen-induced engagement of high-affinity immunoglobulin E receptors (FcepsilonRI) results in the immediate multiubiquitination of FcepsilonRI beta and gamma chains. This ubiquitination is independent of receptor phosphorylation and is restricted to activated receptors. Surprisingly, receptor multiubiquitination is immediately reversible when receptors are disengaged. Therefore, multi-ubiquitination and deubiquitination of FcepsilonRI receptors is controlled at the cell surface by receptor engagement and disengagement. The rapidity, specificity and, most importantly, the reversibility of the activation-induced receptor multiubiquitination suggest that this process may turn on/off a cell surface receptor signaling function thus far unsuspected. RP PAOLINI, R (reprint author), NIAID,MOLEC ALLERGY & IMMUNOL SECT,TWINBROOK BLDG 2,12441 PARKLAWN DR,ROCKVILLE,MD 20852, USA. NR 50 TC 118 Z9 118 U1 0 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD FEB PY 1993 VL 12 IS 2 BP 779 EP 786 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA KL717 UT WOS:A1993KL71700044 PM 8382611 ER PT J AU CORPAS, E HARMAN, SM BLACKMAN, MR AF CORPAS, E HARMAN, SM BLACKMAN, MR TI HUMAN GROWTH-HORMONE AND HUMAN AGING SO ENDOCRINE REVIEWS LA English DT Review ID GH-RELEASING HORMONE; FACTOR-BINDING-PROTEIN; HUMAN-BREAST CANCER; SOMATOMEDIN-C LEVELS; LARON-TYPE DWARFISM; BONE GLA-PROTEIN; LEAN BODY-MASS; RECEIVING PARENTERAL-NUTRITION; FACTOR (IGF)-BINDING PROTEINS; FACTOR-I IMMUNOREACTIVITY C1 NIA, GERONTOL RES CTR,CLIN PHYSIOL LAB,ENDOCRINOL SECT, ROOM 2B19,4940 EASTERN AVE, BALTIMORE, MD 21224 USA. FRANCIS SCOTT KEY MED CTR, DEPT MED, BALTIMORE, MD 21224 USA. JOHNS HOPKINS UNIV, SCH MED, DEPT MED, BALTIMORE, MD 21205 USA. NR 298 TC 668 Z9 675 U1 7 U2 22 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0163-769X EI 1945-7189 J9 ENDOCR REV JI Endocr. Rev. PD FEB PY 1993 VL 14 IS 1 BP 20 EP 39 DI 10.1210/er.14.1.20 PG 20 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KN080 UT WOS:A1993KN08000002 PM 8491152 ER PT J AU MORETTO, M LOPEZ, FJ NEGROVILAR, A AF MORETTO, M LOPEZ, FJ NEGROVILAR, A TI ENDOTHELIN-3 STIMULATES LUTEINIZING-HORMONE-RELEASING HORMONE (LHRH) SECRETION FROM LHRH NEURONS BY A PROSTAGLANDIN-DEPENDENT MECHANISM SO ENDOCRINOLOGY LA English DT Article ID ANTERIOR-PITUITARY-CELLS; VASOCONSTRICTOR PEPTIDE; HYPOTHALAMIC NEURONS; BINDING-SITES; HUMAN-BRAIN; RAT-BRAIN; INVITRO; RECEPTOR; GALANIN; VISUALIZATION AB Endothelin (ET) peptides have recently been recognized as putative regulators of the endocrine system. Particularly in the gonadal system, ET-3 stimulates LH secretion from anterior pituitary cells cultured in vitro. In these studies, we evaluate the actions of ET-3, the most abundant species of the ET family in the central nervous system, on LHRH release from arcuate nucleus-median eminence (AN-ME) fragments and an LHRH-secreting neuronal cell line (GT1 cells) in vitro. ET-3 exhibited a stimulatory effect on LHRH secretion from AN-ME fragments and GT1 cells incubated in a static system as well as in a dynamic perifusion paradigm. In all the systems used, the effects of ET-3 on LHRH secretion showed a dose dependency. The increase in LHRH secretion induced by ET-3 was accompanied by an increased secretion of prostaglandin E2 (PGE2), not only in the AN-ME incubations, but also in the GT1 incubation and perifusion systems. Blockade of arachidonic acid and/or PG synthesis significantly reduced the ET-3-induced LHRH and concomitant PGE2 release from both AN-ME fragments and GT1 cells incubated in vitro. In AN-ME incubations, ET-3 effects were enhanced by potassium-induced depolarization. This suggests that activation of other transmitter system(s) may be needed for obtaining a physiological activation of the LHRH neuronal system. In summary, in these studies we provide evidence for a direct action of ET-3 on the LHRH neuronal system. This action is exerted directly on LHRH neurons either at the level of the nerve terminals, the perikaryon, or both. In addition, the effects of ET-3 on LHRH release require a functional arachidonic acid metabolic pathway, particularly involving PG synthesis, in order to obtain stimulation, indicating that PGs are involved in the intracellular events leading to ET-3-evoked LHRH secretion. RP MORETTO, M (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI,REPROD NEUROENDOCRINOL SECT,BLDG 101,RES TRIANGLE PK,NC 27709, USA. NR 40 TC 37 Z9 38 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1993 VL 132 IS 2 BP 789 EP 794 DI 10.1210/en.132.2.789 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KJ819 UT WOS:A1993KJ81900045 PM 8425494 ER PT J AU LOPEZ, FJ MEADE, EH NEGROVILAR, A AF LOPEZ, FJ MEADE, EH NEGROVILAR, A TI ENDOGENOUS GALANIN MODULATES THE GONADOTROPIN AND PROLACTIN PROESTROUS SURGES IN THE RAT SO ENDOCRINOLOGY LA English DT Article ID HORMONE-RELEASING HORMONE; VASOACTIVE INTESTINAL POLYPEPTIDE; STALK-MEDIAN EMINENCE; POSTERIOR PITUITARY; POSSIBLE INVOLVEMENT; ESTROUS-CYCLE; SECRETION; DOPAMINE; PROGESTERONE; PEPTIDE AB Galanin (GAL) has recently emerged as an important neuroendocrine regulator which participates in the control of several pituitary and hypothalamic hormones. Our earlier observation that GAL stimulates LHRH release from nerve terminals of the median eminence as well as basal LH and LHRH-induced LH secretion from pituitary cells in vitro prompted us to evaluate whether endogenous GAL plays a role in regulation of the physiologically occurring preovulatory surges of gonadotropins and PRL. Proestrous female rats were passively immunized against GAL using a high affinity sheep antirat GAL serum (FMS-FJL 17-5). Animals were implanted during diestrus with indwelling atrial cannulae. On the expected day of proestrus, rats received 1 ml of either normal sheep serum or GAL antiserum (GAL-AS), iv, 1 h before blood sampling started. Blood samples (0.5 ml) were collected at hourly intervals from 1400-2300 h, and plasma levels of LH, FSH, and PRL measured by RIA. At several time intervals after GAL-AS administration, the maximum binding ability of the rat plasma was evaluated using standard saturation assays. High neutralizing levels of immunoglobulins were present throughout the experimental period. GAL passive immunization blunted the LH preovulatory surge by 30%. Although maximum LH levels were unaffected by the treatment, the area under the secretory curve and LH levels at 1700, 1900, and 2000 h were significantly reduced. Conversely, FSH secretion was not significantly altered for either maximum FSH levels or area under the curve. However, FSH levels were significantly diminished in GAL-AS-treated rats at 1700 h. GAL passive immunization selectively reduced the plateau phase of the preovulatory surge of PRL. No significant differences were observed in the initiation of the surge or maximum PRL levels, whereas PRL levels were significantly reduced from 1700 to 2200 h. In addition, the area under the PRL curve was diminished in GAL-AS-treated animals by 40%. In conclusion, our results clearly demonstrate that endogenous GAL is involved in control of the preovulatory surges of LH and PRL without altering the FSH surge. In addition, they provide, for the first time, evidence of an important role for endogenous GAL in the regulation of physiological events leading to ovulation. RP LOPEZ, FJ (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,REPROD NEUROENDOCRINOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 29 TC 80 Z9 80 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1993 VL 132 IS 2 BP 795 EP 800 DI 10.1210/en.132.2.795 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KJ819 UT WOS:A1993KJ81900046 PM 7678800 ER PT J AU LIPOSITS, Z MERCHENTHALER, I REID, JJ NEGROVILAR, A AF LIPOSITS, Z MERCHENTHALER, I REID, JJ NEGROVILAR, A TI GALANIN-IMMUNOREACTIVE AXONS INNERVATE SOMATOSTATIN-SYNTHESIZING NEURONS IN THE ANTERIOR PERIVENTRICULAR NUCLEUS OF THE RAT SO ENDOCRINOLOGY LA English DT Article ID GROWTH-HORMONE SECRETION; CENTRAL-NERVOUS-SYSTEM; DOPAMINE-BETA-HYDROXYLASE; PARAVENTRICULAR NUCLEUS; MEDIAN-EMINENCE; ARCUATE NUCLEUS; IMMUNOHISTOCHEMICAL IDENTIFICATION; CATECHOLAMINERGIC AFFERENTS; TYROSINE-HYDROXYLASE; FACTOR GRF AB The anterior periventricular nucleus (PeN) regulates GH secretion by synthesizing and releasing somatotropin release-inhibiting factor (SRIF) into the portal circulation. This territory of the diencephalon is heavily innervated by axons of galanin (GAL)-immunoreactive (IR) neurons. The connections between GAL-IR fibers and hypophysiotropic SRIF neurons were studied by means of immunocytochemical double labeling at the light and electron microscopic levels. Retrograde axonal labeling with Fluoro-Gold revealed the anterior PeN as the main site of hypophysiotropic SRIF-synthesizing neurons. These cells were densely surrounded by GALergic axons that made contacts with their cell bodies and dendrites. At the ultrastructural level, diaminobenzidine-labeled SRIF neurons received synapsing GAL-IR axons marked with silver-gold particles. Both axo-somatic and axo-dendritic forms of connections were observed. These morphochemical data revealed an interaction between GAL- and SRIF-synthesizing neurons in the anterior PeN. Furthermore, the presence of synaptic connections between these neuronal systems suggests a role for GAL in the control of SRIF secretion and, in turn, in the regulation of GH release. C1 UNIV PECS,SCH MED,DEPT ANAT,ELECTRON MICROSCOP LAB,H-7643 PECS,HUNGARY. RP LIPOSITS, Z (reprint author), NIEHS,MOLEC & INTEGRAT NEUROSCI LAB,FUNCT MORPHOL SECT,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 51 TC 24 Z9 24 U1 1 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1993 VL 132 IS 2 BP 917 EP 923 DI 10.1210/en.132.2.917 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KJ819 UT WOS:A1993KJ81900063 PM 7678803 ER PT J AU ADAMO, ML LANAU, F NEUENSCHWANDER, S WERNER, H LEROITH, D ROBERTS, CT AF ADAMO, ML LANAU, F NEUENSCHWANDER, S WERNER, H LEROITH, D ROBERTS, CT TI DISTINCT PROMOTERS IN THE RAT INSULIN-LIKE GROWTH FACTOR-I (IGF-I) GENE ARE ACTIVE IN CHO CELLS SO ENDOCRINOLOGY LA English DT Note ID LEADER EXONS; EXPRESSION; HORMONE; HEPATOCYTES; REGIONS; ACIDS; RNA AB In mammals, IGF-I mRNAs contain one of two different leader exon sequences that encode different 5'-untranslated regions (UTRs) and signal peptides. The pattern and regulation of expression of these exon 1 and exon 2-derived mRNAs suggests that the expression of each is controlled by a distinct regulatory region. In order to assess this possibility, DNA fragments consisting of sequences flanking and including the exon 1 and 2 transcription initiation sites were cloned into a luciferase expression vector and plasmid DNAs were transiently transfected into Chinese hamster ovary (CHO) cells. A fragment containing approximately 1.1 kb of sequence flanking the most upstream exon 1 transcription initiation site and 362 bp of exon 1 sequence did not stimulate luciferase activity. However, fragments containing 133 bp of 5'-flanking sequence and either 362 or 192 bp of exon 1 sequence stimulated luciferase activity significantly above that seen with a promoterless control plasmid. When the -133/+362 fragment was cloned in the opposite orientation with respect to the luciferase cDNA, the same level of promoter acitivity was observed. Removal of approximately 860 bp from the inactive fragment (i.e., approximately 782 bp of flanking sequence and approximately 74 bp of exon 1 sequence) resulted in promoter activity which was significantly greater than that seen with the promoterless luciferase expression vector, but which was less than that observed with fragments containing the proximal 133 bp of 5'-flanking sequence. Plasmids containing approximately 1.5 kb of flanking sequence and 44 bp of exon 2 sequence also significantly stimulated luciferase activity. These results constitute the first demonstration that both exon 1 and exon 2 transcription start sites are associated with distinct and potentially independently regulatable promoters and provide a molecular basis for the differential expression of these leader exons by developmental, tissue-specific and hormonal factors. RP ADAMO, ML (reprint author), NIDDKD, MOLEC & CELLULAR PHYSIOL SECT, DIABET BRANCH, BETHESDA, MD 20892 USA. NR 16 TC 32 Z9 32 U1 0 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1993 VL 132 IS 2 BP 935 EP 937 DI 10.1210/en.132.2.935 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA KJ819 UT WOS:A1993KJ81900066 PM 8425505 ER PT J AU SCHULICK, RD CLERICI, M DOLAN, MJ SHEARER, GM AF SCHULICK, RD CLERICI, M DOLAN, MJ SHEARER, GM TI LIMITING DILUTION ANALYSIS OF INTERLEUKIN-2-PRODUCING T-CELLS RESPONSIVE TO RECALL AND ALLOANTIGENS IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED AND UNINFECTED INDIVIDUALS SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Article DE LIMITING DILUTION; INTERLEUKIN-2; T-HELPER CELLS; HUMAN IMMUNODEFICIENCY VIRUS ID PERIPHERAL-BLOOD; LYMPHOCYTES-T AB Peripheral blood mononuclear cells (PBMC) from individuals who were seropositive for the human immunodeficiency virus type 1 (HIV), and most without symptoms (HIV+) were compared with PBMC from healthy HIV-seronegative (HIV-) individuals for in vitro generated T helper cell (Th) responses. Th function in bulk culture and limiting dilution analysis was assessed by IL-2 production following stimulation with influenza A virus (FLU) or irradiated allogeneic PBMC (ALLO). We observed that the frequencies of FLU- and ALLO-stimulated Th cells were not appreciably different in the PBMC of HIV-individuals, and that they were also not different in the PBMC of those HIV+ individuals who responded to both FLU and ALLO in bulk culture. However, there was a severe drop in the Th frequency to FLU in HIV+ individuals who were unresponsive to FLU but responsive to ALLO by bulk culture. The PBMC of HIV+ individuals who were unresponsive by bulk culture to both FLU and ALLO exhibited a drastic reduction in the Th frequencies for both stimuli. These results demonstrate a concordance between Th functional analysis performed by limiting dilution and bulk culture. The results also indicate that the early selective loss in Th function to recall antigens is not likely to be due simply to a difference in frequencies of FLU- and ALLO-stimulated Th cells present prior to the onset of Th dysfunction. C1 DEPT INFECT DIS,LACKLAND AFB,TX. RP SCHULICK, RD (reprint author), NCI,EXPTL IMMUNOL BRANCH,BLDG 10,ROOM 4B17,BETHESDA,MD 20892, USA. NR 14 TC 18 Z9 18 U1 0 U2 0 PU VCH PUBLISHERS INC PI DEERFIELD BEACH PA 303 NW 12TH AVE, DEERFIELD BEACH, FL 33442-1788 SN 0014-2980 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD FEB PY 1993 VL 23 IS 2 BP 412 EP 417 DI 10.1002/eji.1830230217 PG 6 WC Immunology SC Immunology GA KM033 UT WOS:A1993KM03300016 PM 8436177 ER PT J AU SANDROCK, D LASTORIA, S MAGRATH, IT NEUMANN, RD AF SANDROCK, D LASTORIA, S MAGRATH, IT NEUMANN, RD TI THE ROLE OF GA-67 TUMOR SCINTIGRAPHY IN PATIENTS WITH SMALL, NON-CLEAVED CELL LYMPHOMA SO EUROPEAN JOURNAL OF NUCLEAR MEDICINE LA English DT Article DE SMALL NON-CLEAVED CELL (BURKITT) LYMPHOMA; GALLIUM SCINTIGRAPHY ID BURKITTS-LYMPHOMA; HODGKINS-DISEASE; GA-67 AB Two hundred and thirty-four scintigraphic studies were performed in 34 patients (27 men, 7 women, age 17.3 +/- 7.7 years) with small, non-cleaved cell lymphoma who had follow-up for 3-96 months (mean 21.6 +/- 21.7 months). Whole-body scintigraphy was performed 48-72 h following i.v. injection of 370 MBq gallium-67 citrate. ''Gold standards'' for truth determinations were surgery, autopsy, histology, axial x-ray computed tomography, magnetic resonance imaging, ultrasonography and clinical follow-up. Overall, 181 of 234 studies were true negative. Eighty proven sites of disease had true positive 67 Ga uptake (in 21 patients/37 studies). Nineteen sites (in 12 patients/15 studies) were false positive. In addition, 31 benign lesions were detected and interpreted correctly in terms of non-malignancy. Ten lymphoma sites (in 6 patients/10 studies) were missed by scintigraphy. Overall, sensitivity of gallium scintigraphy was 89% when calculated by sites and 79% when calculated by studies. Corresponding specificities were 91% and 92%, respectively. Positive predictive values were 81% (sites) and 71% (studies), and negative predictive values 95% (sites and studies). Thus, gallium scintigraphy proved to be a sensitive and specific method for staging and follow-up in patients with small, non-cleaved cell lymphoma. C1 NCI,DEPT NUCL MED,BETHESDA,MD 20892. NCI,DEPT CLIN INVEST,PEDIAT BRANCH,BETHESDA,MD 20892. NR 22 TC 10 Z9 10 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-6997 J9 EUR J NUCL MED JI Eur. J. Nucl. Med. PD FEB PY 1993 VL 20 IS 2 BP 119 EP 122 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA KK843 UT WOS:A1993KK84300006 PM 8440267 ER PT J AU DONEVAN, SD ABRAHAMS, VC AF DONEVAN, SD ABRAHAMS, VC TI CAT TRIGEMINAL NEURONS INNERVATED FROM THE PLANUM-NASALE - THEIR MEDULLARY LOCATION AND THEIR RESPONSES TO MECHANICAL STIMULATION SO EXPERIMENTAL BRAIN RESEARCH LA English DT Article DE TRIGEMINAL NUCLEUS; TACTILE SENSATION; MECHANORECEPTOR; CAT ID GLABROUS SKIN; REPRESENTATION; NUCLEUS; RAT; RHINARIUM; AFFERENTS; CAUDALIS; THALAMUS; NECK AB Experiments were performed to determine whether the receptors of the glabrous skin of the cat-planum nasale (PN) could function in tactile analysis. Trigeminal projection sites of the PN were first identified using transganglionic transport of wheat germ agglutinin-horseradish peroxidase and horseradish peroxidase. Restricted projection sites were identified in this way among the interstitial neurons of the trigeminal tract, in the dorsal horn of the medulla, in subnucleus interpolaris and to a lesser extent in subnucleus oralis. Electrophysiological recording in the trigeminal spinal nucleus confirmed the major neuroanatomical findings and confirmed the paucity of PN projections to the trigeminal system. Most neurons innervated from the PN have small receptive fields, are rapidly adapting and responsive to PN vibration at amplitudes as low as 10 mum. Neurons could be entrained at frequencies below 80 Hz. This upper limit for entrainment presumably reflects the lack of pacinian corpuscles in the PN. A limited number of slowly adapting neurons were found, but only responded to PN displacements of 400 mum and above. The data suggest that the PN can function in tactile analysis to a limited degree. The -significance of these findings is considered with respect to the organization of neural systems controlling head movement. C1 QUEENS UNIV,MRC,DEPT PHYSIOL,SENSORY MOTOR PHYSIOL GRP,KINGSTON K7L 3N6,ONTARIO,CANADA. NINCDS,MED NEUROL BRANCH,BETHESDA,MD 20892. NR 34 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-4819 J9 EXP BRAIN RES JI Exp. Brain Res. PD FEB PY 1993 VL 93 IS 1 BP 66 EP 72 PG 7 WC Neurosciences SC Neurosciences & Neurology GA KN269 UT WOS:A1993KN26900008 PM 7682184 ER PT J AU BRASILNETO, J PASCUALLEONE, A VALLSSOLE, J CAMMAROTA, A COHEN, LG HALLETT, M AF BRASILNETO, J PASCUALLEONE, A VALLSSOLE, J CAMMAROTA, A COHEN, LG HALLETT, M TI POSTEXERCISE DEPRESSION OF MOTOR EVOKED-POTENTIALS - A MEASURE OF CENTRAL-NERVOUS-SYSTEM FATIGUE SO EXPERIMENTAL BRAIN RESEARCH LA English DT Note DE TRANSCRANIAL MAGNETIC STIMULATION; MOTOR EVOKED POTENTIALS; EXERCISE; FATIGUE; HUMAN ID HUMAN-BRAIN; MAGNETIC STIMULATION AB Fatigue of voluntary muscular effort is a complex and multifaceted phenomenon. Fatigue of peripheral nervous system components, including the contractile apparatus and the neuromuscular junction, has been well studied. Central nervous system components also fatigue, but studies have lagged for want of objective methods. Transcranial magnetic stimulation is a relatively new technique that can be used to assess central nervous system excitability from the motor cortex to the alpha-motoneuron. In six normal volunteers, including four of the investigators, the amplitudes of motor evoked potentials elicited by transcranial magnetic stimulation were transiently decreased after exercise, indicating fatigue of motor pathways in the central nervous system. The decrease in amplitude was associated with a feeling of fatigue. The mechanism of this phenomenon is apparently decreased efficiency in the generation of the motor command in the motor cortex. RP HALLETT, M (reprint author), NINCDS, MED NEUROL BRANCH, HUMAN CORT PHYSIOL UNIT, HUMAN MOTOR CONTROL SECT, BLDG 10, BETHESDA, MD 20892 USA. RI Pascual-Leone, Alvaro/G-6566-2011; Brasil-Neto, Joaquim/A-1171-2009 NR 15 TC 166 Z9 166 U1 1 U2 8 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-4819 J9 EXP BRAIN RES JI Exp. Brain Res. PD FEB PY 1993 VL 93 IS 1 BP 181 EP 184 PG 4 WC Neurosciences SC Neurosciences & Neurology GA KN269 UT WOS:A1993KN26900021 PM 8467889 ER PT J AU CROUCH, GD KALEBIC, T TSOKOS, M HELMAN, LJ AF CROUCH, GD KALEBIC, T TSOKOS, M HELMAN, LJ TI ARA-C TREATMENT LEADS TO DIFFERENTIATION AND REVERSES THE TRANSFORMED PHENOTYPE IN A HUMAN RHABDOMYOSARCOMA CELL-LINE SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID CYTOSINE-ARABINOSIDE; 12-O-TETRADECANOYLPHORBOL-13-ACETATE-INDUCED DIFFERENTIATION; CHILDHOOD RHABDOMYOSARCOMA; MYOGENIC DIFFERENTIATION; XENOGRAFTS; CULTURE C1 NCI,PEDIAT BRANCH,BLDG 10 13N240,9000 ROCKVILLE PIKE,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. NR 20 TC 17 Z9 17 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD FEB PY 1993 VL 204 IS 2 BP 210 EP 216 DI 10.1006/excr.1993.1026 PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA KN867 UT WOS:A1993KN86700005 PM 8440318 ER PT J AU YU, ZX BIRO, S FU, YM SANCHEZ, J SMALE, G SASSE, J FERRANS, VJ CASSCELLS, W AF YU, ZX BIRO, S FU, YM SANCHEZ, J SMALE, G SASSE, J FERRANS, VJ CASSCELLS, W TI LOCALIZATION OF BASIC FIBROBLAST GROWTH-FACTOR IN BOVINE ENDOTHELIAL-CELLS - IMMUNOHISTOCHEMICAL AND BIOCHEMICAL-STUDIES SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID BALB/C 3T3 CELLS; EXTRACELLULAR-MATRIX; ANGIOGENIC PROTEIN; COMPLEMENTARY-DNA; FACTOR CDNA; BINDING; SIGNAL; TRANSFORMATION; TRANSLOCATION; SEQUENCE C1 NHLBI,PATHOL BRANCH,BETHESDA,MD 20892. SHRINERS HOSP CRIPPLED CHILDREN,TAMPA,FL. RP YU, ZX (reprint author), NHLBI,CARDIOL BRANCH,10-7B15,BETHESDA,MD 20892, USA. NR 56 TC 57 Z9 61 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4827 J9 EXP CELL RES JI Exp. Cell Res. PD FEB PY 1993 VL 204 IS 2 BP 247 EP 259 DI 10.1006/excr.1993.1031 PG 13 WC Oncology; Cell Biology SC Oncology; Cell Biology GA KN867 UT WOS:A1993KN86700010 PM 8440322 ER PT J AU DUBOIS, CM NETA, R KELLER, JR JACOBSEN, SEW OPPENHEIM, JJ RUSCETTI, F AF DUBOIS, CM NETA, R KELLER, JR JACOBSEN, SEW OPPENHEIM, JJ RUSCETTI, F TI HEMATOPOIETIC GROWTH-FACTORS AND GLUCOCORTICOIDS SYNERGIZE TO MIMIC THE EFFECTS OF IL-1 ON GRANULOCYTE DIFFERENTIATION END IL-1 RECEPTOR INDUCTION ON BONE-MARROW CELLS IN-VIVO SO EXPERIMENTAL HEMATOLOGY LA English DT Article DE IL-1 RECEPTOR; CSFS; GLUCOCORTICOIDS; MYELOPOIESIS ID COLONY-STIMULATING FACTOR; INTERLEUKIN-1 RECEPTOR; CYTOKINE RECEPTORS; INVIVO; EXPRESSION; MICE; MODULATION; FIBROBLASTS; INVITRO; GRANULOPOIESIS AB The mechanisms by which interleukin-1 (IL-1) stimulates hematopoiesis are not clear. We have previously shown that in vivo administration of IL-1 indirectly increases IL-1 receptor (IL-1R) expression on both immature and mature bone marrow (BM) cells, partly due to IL-1-induced hematopoietic growth factor (HGF) production. Because IL-1 also stimulates the hypothalamic pituitary-adrenal axis resulting in the production of glucocorticoids (GC), we assessed whether in vivo treatment with HGF and glucocorticoids upregulates IL-1R, Administration of IL-1 to adrenalectomized mice reduces by 53% IL-specific binding on light density bone marrow (LDBM) cells compared to sham-operated mice. The administration of dexamethasone (dex) alone induced only a slight increase in IL-1R expression but synergized with granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), IL-3 and IL-6 to upregulate IL-1R expression. Flow cytometry analysis using the RB6-8C5 antibody, which is differentially expressed on myeloid cells, indicated that combined G-CSF and dex treatment acts to promote increased numbers of differentiated myeloid progenitors in the bone marrow. Autoradiographic analysis confirmed that while G-CSF and dex increased IL-1R expression on all myeloid cells, it was particularly pronounced for myelocytes, promyelocytes and metamyelocytes. These results suggest that the ability of IL-1 to enhance granulocyte differentiation in vivo is partly due to its ability to induce a cascade of cytokines and steroids which in turn regulate IL-1 receptor expression. C1 NCI,FREDERICK CANC RES & DEV CTR,IMMUNOREGULAT CTR,FREDERICK,MD 21702. ARMED FORCES RADIOBIOL RES INST,DEPT EXPTL HEMATOL,BETHESDA,MD 20814. NCI,DYNCORP INC,FREDERICK CANC RES & DEV CTR,BIOL CARCIOGENESIS & DEV PROGRAM,FREDERICK,MD. FU NCI NIH HHS [N01-CO-74102] NR 40 TC 20 Z9 20 U1 0 U2 0 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD FEB PY 1993 VL 21 IS 2 BP 303 EP 310 PG 8 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA MW607 UT WOS:A1993MW60700021 PM 7678814 ER PT J AU FREDRICKSON, TN HARTLEY, JW MORSE, HC AF FREDRICKSON, TN HARTLEY, JW MORSE, HC TI EARLY DIVERGENCE OF ERYTHROID LINEAGE SUGGESTED BY GENE REARRANGEMENTS IN MOUSE HEMATOPOIETIC NEOPLASMS SO EXPERIMENTAL HEMATOLOGY LA English DT Article DE MOUSE NEOPLASMS; ERYTHROLEUKEMIA MYELOID LEUKEMIA; T CELL LYMPHOMA; B CELL LYMPHOMA; GENE REARRANGEMENTS; MURINE LEUKEMIA VIRUS ID MURINE LEUKEMIA VIRUSES; IMMUNOGLOBULIN; EXPRESSION; CELLS; MICE; LYMPHOMAS AB A total of 113 primary murine hematopoietic neoplasms, including those of erythroid, granulocytic, and T and B lymphoid lineages, were examined for rearrangement of immunoglobulin heavy (IgH) and kappa light chain (IgK) and T cell receptor beta and gamma (TcR-Beta and TcR-gamma) genes. There was a total absence of Ig or TcR gene rearrangements in erythroid leukemias. In contrast, overlaps of IgH rearrangements were observed in myeloid and T cell as well as B cell neoplasms. In a minority of B cell lymphomas, rearrangements of TcR-Beta or TcR-gamma genes were detected. This evidence of shared recombinase activity for myeloid, T cell, and B cell-lineage tumors and the absence of such activity in erythroid tumors suggest early divergence of the erythroid pathway. RP FREDRICKSON, TN (reprint author), NIAID,IMMUNOPATHOL LAB,BLDG 41,RM D311,BETHESDA,MD 20892, USA. OI Morse, Herbert/0000-0002-9331-3705 FU NIAID NIH HHS [N01-AI-72622] NR 26 TC 4 Z9 4 U1 0 U2 0 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD FEB PY 1993 VL 21 IS 2 BP 354 EP 357 PG 4 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA MW607 UT WOS:A1993MW60700029 PM 8425572 ER PT J AU LI, J WIRTZ, RA SCHNEIDER, I MURATOVA, OV MCCUTCHAN, TF APPIAH, A HOLLINGDALE, MR AF LI, J WIRTZ, RA SCHNEIDER, I MURATOVA, OV MCCUTCHAN, TF APPIAH, A HOLLINGDALE, MR TI PLASMODIUM-FALCIPARUM - STAGE-SPECIFIC RIBOSOMAL-RNA AS A POTENTIAL TARGET FOR MONITORING PARASITE DEVELOPMENT IN ANOPHELES-STEPHENSI SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE PLASMODIUM-FALCIPARUM; MALARIA; RIBOSOMAL-RNA PROBE; ANOPHELES-STEPHENSI; MOSQUITO; HYBRIDIZATION ID INFECTED MOSQUITOS; MALARIA; DIAGNOSIS; IDENTIFICATION; PROBE; DNA C1 WALTER REED ARMY INST RES,DEPT ENTOMOL,DIV COMMUNICABLE DIS & IMMUNOL,WASHINGTON,DC 20307. BIOMED RES INST,ROCKVILLE,MD 20852. NIAID,MALARIA RES LAB,BETHESDA,MD 20892. RP LI, J (reprint author), UNIFORMED SERV UNIV HLTH SCI,DEPT PREVENT MED & BIOMETR,4301 JOHNS BRIDGE RD,BETHESDA,MD 20814, USA. NR 20 TC 14 Z9 14 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0014-4894 J9 EXP PARASITOL JI Exp. Parasitol. PD FEB PY 1993 VL 76 IS 1 BP 32 EP 38 DI 10.1006/expr.1993.1004 PG 7 WC Parasitology SC Parasitology GA KP128 UT WOS:A1993KP12800004 PM 8467898 ER PT J AU DUFAU, ML TINAJERO, JC FABBRI, A AF DUFAU, ML TINAJERO, JC FABBRI, A TI CORTICOTROPIN-RELEASING FACTOR - AN ANTIREPRODUCTIVE HORMONE OF THE TESTIS SO FASEB JOURNAL LA English DT Review DE CORTICOTROPIN-RELEASING FACTOR; RECEPTORS/ACTIONS; BETA-ENDORPHIN; OPIOIDS; TESTIS; LEYDIG CELLS; SEROTONERGIC REGULATION; SEROTONIN RECEPTORS ACTION; SIGNALING PATHWAYS; TESTOSTERONE; SEXUAL DYSFUNCTION ID RAT LEYDIG-CELLS; PROOPIOMELANOCORTIN-DERIVED PEPTIDES; PITUITARY-TESTICULAR AXIS; BETA-ENDORPHIN; RESTRAINT STRESS; ACTH-SECRETION; SERTOLI CELLS; RECEPTORS; SEROTONIN; LOCALIZATION AB Corticotropin-releasing factor (CRF), the key neuropeptide in the stress cascade, has major inhibitory actions on testicular function in addition to its known antireproductive effects at the central level (inhibition of sexual behavior and LH secretion). CRF is secreted by the Leydig cells of the testis and acts through high-affinity receptors at the Leydig cell membrane as a potent negative regulator of LH action, inhibiting gonadotropin-induced cAMP generation and androgen production. CRF is also a primary stimulus of beta-endorphin secretion by the Leydig cells, which in turn exerts paracrine inhibition of FSH action in the tubular compartment of the testis through high-affinity receptors in the Sertoli cells. CRF action in the Leydig cells involves a pertussis toxin-insensitive guanyl nucleotide regulatory unit. In contrast to CRF receptors in the brain, pituitary, and other peripheral tissues, those in the Leydig cell are not coupled to G(s). The inhibitory action of CRF in the Leydig cell is exerted through protein kinase C, at the level of the catalytic subunit of adenylate cyclase. The secretion of CRF by the Leydig cell is stimulated by LH, acting via release of serotonin (5HT) and autocrine activation of 5HT2 receptors. Serotonin acts on 5HT2 receptors in the Leydig cell to stimulate CRF secretion via a pertussis toxin insensitive G-protein and presumably through activation of phosphoinositide hydrolysis. The diversity of the biochemical responses to CRF and 5HT2 receptor activation (i.e., inhibition of adenylate cyclase at the cytoplasmic aspect of the cell membrane vs. stimulation of CRF release from secretion granules) may reflect the stimulation of different protein kinase C isoenzymes. The LH --> 5HT --> CRF inhibitory loop serves to continuously buffer the stimulation of androgen production by gonadotropin. 5HT, the immediate stimulus of testicular CRF secretion, is released during stress and is locally increased in the testis in pathological conditions associated with impaired testicular function (i.e., orchitis, varicocele). Also, propranolol, the beta-adrenergic antagonist frequently used in the control of blood pressure in patients with hypertension and often associated with impotence, acts via a serotonergic mechanism to stimulate CRF secretion and causes marked inhibition of LH-induced cAMP production and steroidogenesis in cultured Leydig cells. These basic studies of 5HT and CRF are relevant to the pathogenesis of testicular dysfunction and for the development of antagonist therapies to block CRF production and its local antireproductive effects. RP DUFAU, ML (reprint author), NICHHD, MOLEC ENDOCRINOL SECT, ENDOCRINOL & REPROD RES BRANCH, BLDG 10, RM B1-L400, BETHESDA, MD 20892 USA. NR 73 TC 94 Z9 95 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 1 PY 1993 VL 7 IS 2 BP 299 EP 307 PG 9 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KN885 UT WOS:A1993KN88500005 PM 8382638 ER PT J AU HIROSE, K LONGO, DL OPPENHEIM, JJ MATSUSHIMA, K AF HIROSE, K LONGO, DL OPPENHEIM, JJ MATSUSHIMA, K TI OVEREXPRESSION OF MITOCHONDRIAL MANGANESE SUPEROXIDE-DISMUTASE PROMOTES THE SURVIVAL OF TUMOR-CELLS EXPOSED TO INTERLEUKIN-1, TUMOR-NECROSIS-FACTOR, SELECTED ANTICANCER DRUGS, AND IONIZING-RADIATION SO FASEB JOURNAL LA English DT Note DE MNSOD; IL-1; TNF; ANTICANCER DRUGS; IONIZING RADIATION ID S-TRANSFERASE-PI; RADICAL FORMATION; GLUTATHIONE-PEROXIDASE; NADH DEHYDROGENASE; EXPRESSION VECTOR; OXIDATIVE DAMAGE; CYTO-TOXICITY; RESISTANCE; PARAQUAT; DNA AB Interleukin-1 (IL-1) and tumor necrosis factor (TNF) selectively induce mitochondrial manganese superoxide dismutase (MnSOD) production in various cell types. We have evaluated the capacity of tumor cells that overexpress MnSOD to recover from the cytostatic and cytotoxic effects of cytokines (IL-1 and TNF), chemotherapeutic agents, and ionizing irradiation. Clones of human melanoma cell line, A375, which overexpressed MnSOD after sense MnSOD cDNA transfection, showed increased recovery from treatment with cytostatic and cytotoxic doses of IL-1alpha and TNFalpha, whereas clones of A375 cells that were transfected with anti-sense MnSOD cDNA recovered less well than normal cells from IL-1alpha and TNFalpha. In addition, Chinese hamster ovary (CHO) cells transfected with sense MnSOD cDNA showed increased survival after treatment with doxorubicin, mitomycin C, and gamma (gamma) radiation in vitro. It is hypothesized that mitochondrial MnSOD, by scavenging oxygen radicals induced by cytokines, some cytotoxic drugs, and ionizing radiation, is protective and promotes the survival of cells from the lethal effects of these treatments. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,MOLEC IMMUNOREGULAT LAB,FREDERICK,MD 21702. NR 46 TC 310 Z9 320 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD FEB 1 PY 1993 VL 7 IS 2 BP 361 EP 368 PG 8 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA KN885 UT WOS:A1993KN88500011 PM 8440412 ER PT J AU MARCO, S VALPUESTA, JM RIVAS, G ANDRES, G MARTIN, CS CARRASCOSA, JL AF MARCO, S VALPUESTA, JM RIVAS, G ANDRES, G MARTIN, CS CARRASCOSA, JL TI A STRUCTURAL MODEL FOR THE GROEL CHAPERONIN SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE GROEL CHAPERONE; ELECTRON MICROSCOPY; IMAGE PROCESSING ID MOLECULAR CHAPERONES; ELECTRON-MICROSCOPY; ESCHERICHIA-COLI; PROTEIN; PURIFICATION; CARBOXYLASE; HEAD AB Individual particle analysis of end views from negatively stained specimens of purified GroEL from Escherichia coli showed the presence of two different particle populations, those with a six-fold symmetry and those with a seven-fold symmetry, when studied at pH 7.7 and 5.0. Image processing of particles from frozen-hydrated specimens revealed at both pH values a homogeneous population of particles with a strong seven-fold symmetry component and an average image with seven asymmetric units. Biochemical analysis of purified GroEL showed unequivocally the presence of a single polypeptide with the N-terminal sequence identical to that of GroEL. These results are compatible with a structural model of GroEL as an asymmetric aggregate built up by two rings of seven-fold and six-fold symmetries, respectively. C1 NIH,DEPT HLTH & HUMAN SERV,BETHESDA,MD 20892. RI San Martin, Carmen/A-4074-2010; Andres, German/E-2632-2016; OI San Martin, Carmen/0000-0001-9799-175X; Andres, German/0000-0003-0265-5409; Rivas, German/0000-0003-3450-7478 NR 24 TC 7 Z9 7 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1097 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD FEB 1 PY 1993 VL 106 IS 3 BP 301 EP 308 DI 10.1111/j.1574-6968.1993.tb05980.x PG 8 WC Microbiology SC Microbiology GA KN731 UT WOS:A1993KN73100012 PM 8095912 ER PT J AU SPIRTAS, R KAUFMAN, SC ALEXANDER, NJ AF SPIRTAS, R KAUFMAN, SC ALEXANDER, NJ TI FERTILITY DRUGS AND OVARIAN-CANCER - RED ALERT OR RED HERRING SO FERTILITY AND STERILITY LA English DT Editorial Material DE FEMALE INFERTILITY; FERTILITY DRUGS; OVARIAN CANCER; OVULATION INDUCTION ID FOLLICULAR STIMULATION; GONADOTROPIN THERAPY; INCESSANT OVULATION; CARCINOMA C1 NICHHD,CTR POPULAT RES,CONTRACEPT DEV BRANCH,BETHESDA,MD 20892. RP SPIRTAS, R (reprint author), NICHHD,CTR POPULAT RES,CONTRACEPT & REPROD EVALUAT BRANCH,BLDG 61E,ROOM 8B07,BETHESDA,MD 20892, USA. NR 23 TC 48 Z9 48 U1 2 U2 2 PU AMER SOC REPRODUCTIVE MEDICINE PI BIRMINGHAM PA 1209 MONTGOMERY HIGHWAY, BIRMINGHAM, AL 35216-2809 SN 0015-0282 J9 FERTIL STERIL JI Fertil. Steril. PD FEB PY 1993 VL 59 IS 2 BP 291 EP 293 PG 3 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA KK765 UT WOS:A1993KK76500004 PM 8425620 ER PT J AU BATISTA, MC CARTLEDGE, TP MERINO, MJ AXIOTIS, C PLATIA, MP MERRIAM, GR LORIAUX, DL NIEMAN, LK AF BATISTA, MC CARTLEDGE, TP MERINO, MJ AXIOTIS, C PLATIA, MP MERRIAM, GR LORIAUX, DL NIEMAN, LK TI MIDLUTEAL PHASE ENDOMETRIAL BIOPSY DOES NOT ACCURATELY PREDICT LUTEAL FUNCTION SO FERTILITY AND STERILITY LA English DT Article DE PROGESTERONE; ENDOMETRIUM; ENDOMETRIAL BIOPSY; LUTEAL PHASE; LUTEAL INSUFFICIENCY; LUTEAL PHASE DEFECT ID INFERTILE WOMEN; PLASMA PROGESTERONE; MENSTRUAL-CYCLE; CORPUS-LUTEUM; DIAGNOSIS; DEFICIENCY; INADEQUACY; OVULATION; DEFECTS AB Objective: To investigate whether a midluteal phase endometrial biopsy accurately predicts luteal function. Design: One nonpregnant menstrual cycle was evaluated in a prospective fashion. Setting: Outpatient Clinic of the Clinical Center of the National Institutes of Health. Participants: Fifty healthy, normally cycling women. Interventions: Serum progesterone (P) was measured daily throughout the luteal phase. An endometrial biopsy was performed 7 to 9 days after the luteinizing hormone (LH) surge, as detected by rapid plasma assays, and dated histologically according to Noyes' criteria. Main Outcome Measure: To correlate endometrial maturation with luteal P secretion. Results: Mean integrated P measurements were reduced only when the lag between histologic and chronological dating was greater-than-or-equal-to 3 days or greater-than-or-equal-to 4 days, depending on whether chronological dates were assigned prospectively from the LH surge or retrospectively from the onset of next menses, respectively. However, these lags did not consistently predict deficient luteal function because subnormal integrated P secretion was seen in only 14% of women with these delays in endometrial maturation. Conclusions: Midluteal phase endometrial biopsy provides a crude test of luteal function that does not precisely distinguish luteal insufficiency. C1 NICHHD,WARREN GRANT MAGNUSON CLIN CTR,DEV ENDOCRINOL BRANCH,BLDG 10,BETHESDA,MD 20892. NICHHD,WARREN GRANT MAGNUSON CLIN CTR,DEPT NURSING,BETHESDA,MD 20892. NCI,DIV CANC BIOL & DIAGNOST,PATHOL LAB,BETHESDA,MD 20892. NR 25 TC 24 Z9 25 U1 0 U2 0 PU AMER SOC REPRODUCTIVE MEDICINE PI BIRMINGHAM PA 1209 MONTGOMERY HIGHWAY, BIRMINGHAM, AL 35216-2809 SN 0015-0282 J9 FERTIL STERIL JI Fertil. Steril. PD FEB PY 1993 VL 59 IS 2 BP 294 EP 300 PG 7 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA KK765 UT WOS:A1993KK76500005 PM 8425621 ER PT J AU VANDERZEE, J KROOTJES, BBH CHIGNELL, CF DUBBELMAN, TMAR VANSTEVENINCK, J AF VANDERZEE, J KROOTJES, BBH CHIGNELL, CF DUBBELMAN, TMAR VANSTEVENINCK, J TI HYDROXYL RADICAL GENERATION BY A LIGHT-DEPENDENT FENTON REACTION SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE IRON; IRON CHELATORS; ULTRAVIOLET RADIATION; HYDROXYL RADICAL; PHOTOCHEMISTRY; ESR SPECTROSCOPY; FREE RADICALS ID ELECTRON-SPIN-RESONANCE; HYDROGEN-PEROXIDE; AQUEOUS-SOLUTION; SUPEROXIDE; REDUCTION; IRON; ULTRAVIOLET; RADIATIONS; FERRITIN; RELEASE AB Illumination of Fe3+, with light of a wavelength varying from 250 to 450 nm, in the presence of the iron chelators ethylenediamine N,N,N',N'-tetraacetic acid (EDTA), ethyleneglycol-bis-(beta-aminoethylether)N,N,N',N'-tetraacetic acid (EGTA), diethylenetriamine-N,N,N',N',N'-pentaacetic acid (DTPA), or citrate resulted in the reduction of Fe3+ to Fe2+.Fe2+ formation was measured by the formation of its complex with bathophenanthroline disulfonic acid. In all cases Fe2+ formation was completely dependent on the presence of the iron chelator and on the wavelength used for illumination. A correlation was found between the absorption spectrum of the iron-chelator complex and the amount of Fe3+ reduced, suggesting that the absorption of light induced an electron transfer from the chelator to the iron ion. Exposure to oxygen, either during or after illumination, resulted in degradation of the chelator molecule. Illumination of the Fe3+-chelator complexes in the presence of H2O2 resulted in the formation of hydroxyl radicals, which could be determined by the formation of the 5,5-dimethyl-1-pyrroline N-oxide (DMPO)-hydroxyl radical adduct, using electron spin resonance spectroscopy. Formation of the spin adduct was inhibited by addition of catalase, mannitol, ethanol, or formate, whereas superoxide dismutase had no effect. C1 NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709. RP VANDERZEE, J (reprint author), SYLVIUS LAB,DEPT MED BIOCHEM,POB 9503,2300 RA LEIDEN,NETHERLANDS. NR 29 TC 40 Z9 40 U1 3 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD FEB PY 1993 VL 14 IS 2 BP 105 EP 113 PG 9 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA KJ348 UT WOS:A1993KJ34800001 PM 8381101 ER PT J AU BACON, BR DIBISCEGLIE, AM AF BACON, BR DIBISCEGLIE, AM TI ASPARTATE-AMINOTRANSFERASE AND IRON STATUS - LACK OF SUPPORT FOR COVARIATION - REPLY SO GASTROENTEROLOGY LA English DT Letter C1 NIDDKD,HEPATITIS STUDIES SECT,BETHESDA,MD 20892. RP BACON, BR (reprint author), ST LOUIS UNIV,MED CTR,ST LOUIS,MO 63103, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD FEB PY 1993 VL 104 IS 2 BP 665 EP 665 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA KM861 UT WOS:A1993KM86100044 ER PT J AU SAXE, CL GINSBURG, GT LOUIS, JM JOHNSON, R DEVREOTES, PN KIMMEL, AR AF SAXE, CL GINSBURG, GT LOUIS, JM JOHNSON, R DEVREOTES, PN KIMMEL, AR TI CAR2, A PRESTALK CAMP RECEPTOR REQUIRED FOR NORMAL TIP FORMATION AND LATE DEVELOPMENT OF DICTYOSTELIUM-DISCOIDEUM SO GENES & DEVELOPMENT LA English DT Article DE CAMP; RECEPTORS; GENE EXPRESSION; DICTYOSTELIUM ID CYCLIC-AMP RECEPTORS; CELL-SURFACE; GENE-EXPRESSION; INOSITOL TRISPHOSPHATE; ADENYLATE-CYCLASE; ALPHA-SUBUNITS; INDUCTION; DIFFERENTIATION; CHEMOATTRACTANT; IDENTIFICATION AB Extracellular cAMP serves as a primary signaling molecule to regulate the development of Dictyostelium discoideum. It is required for chemotaxis, aggregation, cytodifferentiation, and morphogenetic movement. The receptors for cAMP are members of the family of cell-surface receptors that are linked to G proteins and characterized by seven putative transmembrane domains. Previously, we have isolated the gene for the cAMP receptor subtype 1 (CAR1) from Dictyostelium and suggested that several genes related to CAR1 were present in the genome. Here, we describe a family of cAMP receptor genes of Dictyostelium and the isolation and function of the gene for the cAMP receptor subtype 2, CAR2. CAR2 is structurally similar to CAR1. Overall, their transmembrane and loop domains are approximately 75% identical in amino acid sequence; however, their carboxyl termini are quite dissimilar; CAR2 possesses homopolymeric runs of histidines and asparagines that are absent from the corresponding region in CAR1. Although CAR1 is maximally expressed during the early stages of development, CAR2 is expressed only after cells have aggregated and, then, preferentially in prestalk cells. Transgenic Dictyostelium that have had their wild-type CAR2 gene replaced by a defective copy using homologous recombination proceed through early development but are detained at the tight mound stage. CAR2 may be required for cAMP-directed sorting of prestalk cells during pattern formation within the aggregation mound. Furthermore, although prestalk genes are expressed normally in aggregates that lack CAR2, they exhibit an enhanced expression of prespore-specific mRNA. Previously, we had shown that there was a requirement for CAR1 during early development. The present results demonstrate that the multiple responses of Dictyostelium to cAMP are regulated by distinct cAMP receptors that are encoded by unique genes. C1 NIDDK,CELLULAR & DEV BIOL LAB,BLDG 6-B1-22,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205. NR 42 TC 114 Z9 115 U1 1 U2 4 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0890-9369 J9 GENE DEV JI Genes Dev. PD FEB PY 1993 VL 7 IS 2 BP 262 EP 272 DI 10.1101/gad.7.2.262 PG 11 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA KM859 UT WOS:A1993KM85900010 PM 8436297 ER PT J AU JOHNSON, RL SAXE, CL GOLLOP, R KIMMEL, AR DEVREOTES, PN AF JOHNSON, RL SAXE, CL GOLLOP, R KIMMEL, AR DEVREOTES, PN TI IDENTIFICATION AND TARGETED GENE DISRUPTION OF CAR3, A CAMP RECEPTOR SUBTYPE EXPRESSED DURING MULTICELLULAR STAGES OF DICTYOSTELIUM DEVELOPMENT SO GENES & DEVELOPMENT LA English DT Article DE CAMP; RECEPTORS; DICTYOSTELIUM; MORPHOGENESIS ID BETA-ADRENERGIC-RECEPTOR; CYCLIC-AMP RECEPTOR; CELL-SURFACE; BETA-2-ADRENERGIC RECEPTOR; DEPENDENT PHOSPHORYLATION; BINDING-SITES; ACTIN GENE; DISCOIDEUM; PROTEIN; RHODOPSIN AB Extracellular cAMP acts through cell-surface receptors to coordinate the developmental program of Dictyostelium. A cAMP receptor (cAR1), which is expressed during early aggregation, has been cloned and sequenced previously. We have identified a new receptor subtype, cAR3, that has approximately 56% and 69% amino acid identity with cAR1 and cAR2, respectively. cAR1, cAR2, or cAR3 expressed from plasmid in growing Dictyostelium cells can be photoaffinity labeled with 8-N3[P-32]cAMP and phosphorylated when stimulated with cAMP. cAR3 RNA was not present during growth but appeared during late aggregation. Its expression peaked at 9 hr and then fell to a reduced level that was maintained until culmination. The expression of cAR3 protein followed a similar pattern, but with a 3-hr lag, and reached a maximum at the mound stage. In contrast, cAR1 protein was expressed predominantly during early aggregation and at low levels during later stages. At their respective peaks of expression, there were approximately 5 x 10(3) cAR3 sites per cell compared with approximately 7 x 10(4) cAR1 sites per cell. The cAR3 gene was disrupted by homologous recombination in several different parental cell lines. Surprisingly, the car3- cell lines display no obvious phenotype. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,BALTIMORE,MD 21205. NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892. FU NIGMS NIH HHS [GM34933] NR 47 TC 63 Z9 64 U1 1 U2 4 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0890-9369 J9 GENE DEV JI Genes Dev. PD FEB PY 1993 VL 7 IS 2 BP 273 EP 282 DI 10.1101/gad.7.2.273 PG 10 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA KM859 UT WOS:A1993KM85900011 PM 8382181 ER PT J AU DELAMO, FF GENDRONMAGUIRE, M SWIATEK, PJ JENKINS, NA COPELAND, NG GRIDLEY, T AF DELAMO, FF GENDRONMAGUIRE, M SWIATEK, PJ JENKINS, NA COPELAND, NG GRIDLEY, T TI CLONING, ANALYSIS, AND CHROMOSOMAL LOCALIZATION OF NOTCH-1, A MOUSE HOMOLOG OF DROSOPHILA NOTCH SO GENOMICS LA English DT Article ID CELL-CYCLE CONTROL; HUMAN ERYTHROCYTE ANKYRIN; GENETIC-LINKAGE MAP; CONTROL PROTEINS; C-ELEGANS; LOCUS; MELANOGASTER; SEQUENCES; REPEATS; DIFFERENTIATION C1 ROCHE INST MOLEC BIOL,DEPT CELL & DEV BIOL,NUTLEY,NJ 07110. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. FU NCI NIH HHS [N01-CO-74101] NR 52 TC 90 Z9 91 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1993 VL 15 IS 2 BP 259 EP 264 DI 10.1006/geno.1993.1055 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA KP145 UT WOS:A1993KP14500002 PM 8449489 ER PT J AU PAROLINI, O HEJTMANCIK, JF ALLEN, RC BELMONT, JW LASSITER, GL HENRY, MJ BARKER, DF CONLEY, ME AF PAROLINI, O HEJTMANCIK, JF ALLEN, RC BELMONT, JW LASSITER, GL HENRY, MJ BARKER, DF CONLEY, ME TI LINKAGE ANALYSIS AND PHYSICAL MAPPING NEAR THE GENE FOR X-LINKED AGAMMAGLOBULINEMIA AT XQ22 SO GENOMICS LA English DT Article ID CARRIER DETECTION; CLOSE LINKAGE; CHROMOSOME INACTIVATION; DNA MARKERS; B-CELLS; IMMUNODEFICIENCY; LOCUS; POLYMORPHISM; DXS178 C1 ST JUDE CHILDRENS RES HOSP, DEPT IMMUNOL, 332 N LAUDERDALE, MEMPHIS, TN 38101 USA. UNIV TENNESSEE, CTR HLTH SCI, COLL MED, DEPT PEDIAT, MEMPHIS, TN 38163 USA. NEI, BETHESDA, MD 20892 USA. BAYLOR COLL MED, INST MOLEC GENET, HOWARD HUGHES MED INST, HOUSTON, TX 77030 USA. UNIV UTAH, DEPT MED INFORMAT, SALT LAKE CITY, UT 84112 USA. OI Belmont, John/0000-0001-7409-3578 FU NCI NIH HHS [P30 CA21765]; NIAID NIH HHS [AI 25129] NR 31 TC 44 Z9 47 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1993 VL 15 IS 2 BP 342 EP 349 DI 10.1006/geno.1993.1066 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA KP145 UT WOS:A1993KP14500013 PM 8449500 ER PT J AU GARTNER, J KEARNS, W ROSENBERG, C PEARSON, P COPELAND, NG GILBERT, DJ JENKINS, NA VALLE, D AF GARTNER, J KEARNS, W ROSENBERG, C PEARSON, P COPELAND, NG GILBERT, DJ JENKINS, NA VALLE, D TI LOCALIZATION OF THE 70-KDA PEROXISOMAL MEMBRANE-PROTEIN TO HUMAN 1P21-P22 AND MOUSE-3 SO GENOMICS LA English DT Note ID ZELLWEGER SYNDROME; CHROMOSOME-7; FAMILY; GENE C1 JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,PCTB 802,725 N WOLFE ST,BALTIMORE,MD 21205. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701. JOHNS HOPKINS UNIV,SCH MED,KENNEDY KRIEGER INST,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,DEPT MED,BALTIMORE,MD 21205. JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST,BALTIMORE,MD 21205. RI rosenberg, carla/N-4148-2015 OI rosenberg, carla/0000-0003-3253-4448 FU NCI NIH HHS [N01-CO-74101]; NICHD NIH HHS [P30HD10981-16, P30HD27799] NR 19 TC 18 Z9 18 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1993 VL 15 IS 2 BP 412 EP 414 DI 10.1006/geno.1993.1076 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA KP145 UT WOS:A1993KP14500023 PM 8449508 ER PT J AU PETTY, EM ARNOLD, A MARX, SJ BALE, AE AF PETTY, EM ARNOLD, A MARX, SJ BALE, AE TI A PULSED-FIELD GEL-ELECTROPHORESIS (PFGE) MAP OF 12 LOCI ON CHROMOSOME-11Q11-Q13 SO GENOMICS LA English DT Note ID SMALL REGION; LONG ARM; ONCOGENE; LOCALIZATION; BCL-1; GENE C1 MASSACHUSETTS GEN HOSP,ENDOCRINE UNIT,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02115. NIDDKD,METAB DIS BRANCH,BETHESDA,MD 20892. RP PETTY, EM (reprint author), YALE UNIV,SCH MED,DEPT FORENS MED,NEW HAVEN,CT 06510, USA. FU NCI NIH HHS [CA55909, CA50497-04]; NIGMS NIH HHS [GM07439] NR 15 TC 8 Z9 8 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD FEB PY 1993 VL 15 IS 2 BP 423 EP 425 DI 10.1006/geno.1993.1080 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA KP145 UT WOS:A1993KP14500027 PM 8449512 ER PT J AU SCHNELLE, JF NEWMAN, D WHITE, M ABBEY, J WALLSTON, KA FOGARTY, T ORY, MG AF SCHNELLE, JF NEWMAN, D WHITE, M ABBEY, J WALLSTON, KA FOGARTY, T ORY, MG TI MAINTAINING CONTINENCE IN NURSING-HOME RESIDENTS THROUGH THE APPLICATION OF INDUSTRIAL QUALITY-CONTROL SO GERONTOLOGIST LA English DT Article DE STAFF MANAGEMENT; CARE BEHAVIORS ID URINARY-INCONTINENCE; HEALTH-CARE; MANAGEMENT; FACILITIES; STAFF; SYSTEM; REDUCE AB Supervisory nurses in seven nursing homes were taught how to implement a quality control management system that permitted the time-efficient assessment of how well a prompted voiding toileting program continued to be implemented by indigenous nursing aides. Random-hour wet checks taken during the 6-month management period revealed a reduction in wetness from 43 % to 21 % < .0001) which was sustained over the full 6-month period. C1 VANDERBILT UNIV, SCH NURSING, NASHVILLE, TN 37240 USA. UNIV CALIF BERKELEY, DEPT PUBL HLTH, BERKELEY, CA 94720 USA. NIA, BETHESDA, MD 20892 USA. MIDDLE TENNESSEE STATE UNIV, DEPT PSYCHOL, MURFREESBORO, TN 37130 USA. RP SCHNELLE, JF (reprint author), UNIV CALIF LOS ANGELES, SCH MED, BORUN CTR GERONTOL RES, 10833 LE CONTE AVE, CHS 32-144, LOS ANGELES, CA 90024 USA. FU NIA NIH HHS [5U01 AG05270] NR 23 TC 43 Z9 43 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0016-9013 EI 1758-5341 J9 GERONTOLOGIST JI Gerontologist PD FEB PY 1993 VL 33 IS 1 BP 114 EP 121 PG 8 WC Gerontology SC Geriatrics & Gerontology GA KL209 UT WOS:A1993KL20900014 PM 8440494 ER PT J AU XUAN, XZ LUBIN, JH LI, JY YANG, LF LUO, QS YANG, L WANG, JZ BLOT, WJ AF XUAN, XZ LUBIN, JH LI, JY YANG, LF LUO, QS YANG, L WANG, JZ BLOT, WJ TI A COHORT STUDY IN SOUTHERN CHINA OF TIN MINERS EXPOSED TO RADON AND RADON DECAY PRODUCTS SO HEALTH PHYSICS LA English DT Article DE EPIDEMIOLOGY; EXPOSURE, RADIATION; CANCER; RADON PROGENY ID LUNG-CANCER MORTALITY; COPPER SMELTER WORKERS; STATES URANIUM MINERS; CIGARETTE-SMOKING; DAUGHTER EXPOSURE; YUNNAN PROVINCE; PROGENY; RISK; RADIATION; MODELS AB Several studies of underground miners have demonstrated that exposure to radioactive radon gas (more precisely, Rn-222 and its short-lived decay products) at levels historically found in mines increases the risk of lung cancer. Because of small numbers of lung cancers, previous studies have had limited power to evaluate temporal and other characteristics of patterns of risk. Herein we report on a historical cohort study of male employees of the Yunnan Tin Corporation in southern China. The cohort consists of 17,143 workers with 175,143 person-years of observation and 981 lung cancer events. Eighty percent of the workers were employed underground and exposed to radon. The excess relative risk increased linearly with exposure, rising 0.6% per working level month (95% confidence interval = 0.4-0.8). In the mines, workers were also exposed to arsenic-containing dusts. Adjustment for arsenic exposure, a known lung carcinogen, reduced the effect of radon exposure to 0.2% per working level month (95% confidence interval = 0.1-0.2). The excess relative risk/working level month declined significantly with attained age and with radon exposure rate as measured by the cumulative working level month divided by duration of exposure. It also declined significantly with years from last exposure and with time since exposure, but these declines were consistent only after adjustment for arsenic exposure. In this cohort, 41% of the underground workers were first exposed when <15 y old; however, lung cancer risk did not vary consistently with age at first radon exposure. A joint analysis of radon exposure and smoking status (smoker vs. nonsmoker) rejected both an additive and a multiplicative association; the relationship was consistent with an intermediate association. C1 NCI, EPN, BIOSTAT BRANCH, ROOM 403, 6130 EXECUT BLVD, ROCKVILLE, MD 20892 USA. YUNNAN TIN CORP, INST LABOR PROTECT, GEJIU, PEOPLES R CHINA. CHINESE ACAD MED SCI, DEPT EPIDEMIOL, BEIJING, PEOPLES R CHINA. NR 35 TC 59 Z9 64 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0017-9078 EI 1538-5159 J9 HEALTH PHYS JI Health Phys. PD FEB PY 1993 VL 64 IS 2 BP 120 EP 131 PG 12 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA KH695 UT WOS:A1993KH69500002 PM 8449705 ER PT J AU TAYLOR, GN LLOYD, RD MAYS, CW AF TAYLOR, GN LLOYD, RD MAYS, CW TI LIVER-CANCER INDUCTION BY PU-239, AM-241, AND THOROTRAST IN THE GRASSHOPPER MOUSE, ONYCHOMYS LEUKOGASTER SO HEALTH PHYSICS LA English DT Article DE LABORATORY ANIMALS; CANCER; TUMORS; RADIATION EFFECTS AB Forty young adult grasshopper mice (Onychomys leukogaster) of both genders were injected with either 129 or 44 kBq kg-1 of monomeric Pu-239 and were maintained for lifetime observation. Average liver doses to death (mean times +/- standard deviation (SD) from injection to death = 405 +/-133 and 756 +/- 189 d) were calculated as approximately 16 and 9 Gy, respectively. These animals developed a total of 18 primary liver tumors (neoplasms, malignant, and benign). Comparison of these mice to a previously published study involving 49 control animals of the same species, 70 mice given Am-241, and 73 given Thorotrast, indicated that the liver cancer induction of Thorotrast can be attributed almost exclusively to the effects of the radioactivity and not to its nonradiation properties. This suggests that projected risks of liver cancer induction from Pu-239, Am-241, or other liver-seeking actinides in humans probably can be estimated from the liver cancer experience in Thorotrast patients using the calculated radiation doses to liver. For this species, the linear risk coefficient for induction of liver neoplasia (percent of mice with liver tumor) by Am-241 or Thorotrast was estimated to be about 14.6 +/- 5.4 times the average liver dose (in Gy) for groups of animals with average liver doses of 5 Gy or less. The lowest average liver dose among groups of these mice given Pu-239 was about 9 Gy, the dose was not in the linear range, and it was too high to yield reliable results for determining a risk coefficient for low dose irradiation. However, the estimates for a risk coefficient were similar for the plutonium and americium mice with liver doses of approximately 9 Gy or 16 Gy. C1 UNIV UTAH, RADIOBIOL LAB, BLDG 586, SALT LAKE CITY, UT 84112 USA. NCI, BETHESDA, MD 20892 USA. NR 22 TC 16 Z9 16 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0017-9078 EI 1538-5159 J9 HEALTH PHYS JI Health Phys. PD FEB PY 1993 VL 64 IS 2 BP 141 EP 146 DI 10.1097/00004032-199302000-00003 PG 6 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA KH695 UT WOS:A1993KH69500004 PM 8449707 ER PT J AU GREVER, M MALSPEIS, L AF GREVER, M MALSPEIS, L TI THE DISCOVERY AND DEVELOPMENT OF NEW ANTILEUKEMIC DRUGS SO HEMATOLOGY-ONCOLOGY CLINICS OF NORTH AMERICA LA English DT Review ID ACUTE PROMYELOCYTIC LEUKEMIA; HAIRY-CELL LEUKEMIA; DNA TOPOISOMERASE-I; ACUTE NONLYMPHOCYTIC LEUKEMIA; CHRONIC MYELOGENOUS LEUKEMIA; CHRONIC LYMPHOCYTIC-LEUKEMIA; TRANS RETINOIC ACID; BREFELDIN-A; CC-1065 NSC-298223; MYELOID-LEUKEMIA C1 FREDERICK CANC RES & DEV CTR,FREDERICK,MD. RP GREVER, M (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,EXECUT PLAZA N,ROOM 843,BETHESDA,MD 20892, USA. NR 110 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0889-8588 J9 HEMATOL ONCOL CLIN N JI Hematol. Oncol. Clin. North Am. PD FEB PY 1993 VL 7 IS 1 BP 233 EP 254 PG 22 WC Oncology; Hematology SC Oncology; Hematology GA KL332 UT WOS:A1993KL33200011 PM 8449860 ER PT J AU OLDFIELD, EH RAM, Z CULVER, KW BLAESE, RM DEVROOM, HL AF OLDFIELD, EH RAM, Z CULVER, KW BLAESE, RM DEVROOM, HL TI GENE-THERAPY FOR THE TREATMENT OF BRAIN-TUMORS USING INTRA-TUMORAL TRANSDUCTION WITH THE THYMIDINE KINASE GENE AND INTRAVENOUS GANCICLOVIR SO HUMAN GENE THERAPY LA English DT Article ID GLIOMAS; RECOMBINATION; REOPERATION; METASTASES; VECTORS; TRIAL AB Malignant brain tumors are responsible for significant morbidy and mortality in both pediatric and adult populations. These common tumors present an enormous therapeutic challenge due to their poor outcome despite radical surgery, high dose radiotherapy and chemotherapy. Survival of patients from the time of diagnosis is measured in months and recurrence after treatment is associated with a life expectancy of weeks. In an attempt to improve this grim prognosis of patients with malignant brain tumors (both primary tumors and secondary metastasis from systemic cancer such as melanoma, lung and breast cancer), we have developed a novel approach to the therapy of brain tumors. This approach makes use of recombinant DNA technology to transfer a sensitivity gene into a brain tumor. This is achieved by direct injection of the tumor with a cell line actively producing a retroviral vector carrying a gene conferring drug sensitivity to the tumor. A retroviral vector is a mouse retrovirus genetically engineered to replace its own genes with a new gene. Such vectors are capable of ''infecting'' mammalian cells and stably incorporate their new genetic material into the genome of the infected host. The producer cell is an NIH 3T3 cell that has been genetically engineered to continually produce retroviral vectors. The new gene is incorporated into the genome of the tumor cells and expresses the protein which is encoded by the new gene. This protein (the herpes simplex virus enzyme thymidine kinase, HS-tk) sensitizes the tumor cells to an antiviral drug (ganciclovir, GCV) which is a natural substrate for HS-tk. The enzymatic process induced by GCV leads to death of the cell expressing the herpes TK activity, i.e., death of the tumor cells. Since the HS-tk enzyme which is normally present in mammalian cells has very low affinity for GCV, systemic toxicity related to this mechanism is not observed. This type of in vivo gene transfer has several unique features. First these retroviral-vectors will only integrate and express their genes in cells which are actively synthesizing DNA. Therefore, surrounding non-proliferating normal brain tissue should not acquire the HS-tk gene and will remain insensitive to GCV. Second, all of the transduced tumor cells (and retroviral vector producing cells) will be killed by the host immune response and/or GCV treatment eliminating potential concern about insertional mutagenesis giving rise to malignant cells. This is the first clinical attempt to treat malignant tumors in human beings by in-vivo genetic manipulation of the tumor's genome. C1 NCI,DCBDC,METAB BRANCH,CELLULAR IMMUNOL SECT,BETHESDA,MD 20892. NINCDS,SNB,BETHESDA,MD 20892. NHLBI,MHB,BETHESDA,MD 20892. RP OLDFIELD, EH (reprint author), NINCDS,SURG NEUROL BRANCH,BETHESDA,MD 20892, USA. NR 20 TC 382 Z9 392 U1 0 U2 5 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD FEB PY 1993 VL 4 IS 1 BP 39 EP 69 DI 10.1089/hum.1993.4.1-39 PG 31 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA KP288 UT WOS:A1993KP28800007 PM 8384892 ER PT J AU YI, HF DONOHUE, SJ KLEIN, DC MCBRIDE, OW AF YI, HF DONOHUE, SJ KLEIN, DC MCBRIDE, OW TI LOCALIZATION OF THE HYDROXYINDOLE-O-METHYLTRANSFERASE GENE TO THE PSEUDOAUTOSOMAL REGION - IMPLICATIONS FOR MAPPING OF PSYCHIATRIC-DISORDERS SO HUMAN MOLECULAR GENETICS LA English DT Article ID HUMAN SEX-CHROMOSOMES; HUMAN GENOME; LINKAGE MAP; MELATONIN; LIGHT; MINISATELLITES; SEQUENCES; PSYCHOSIS; LOCUS; CDNA AB The human hydroxyindole-O-methyltransferase (HIOMT) gene has been mapped to the X chromosome using cDNA probes from the 3' and 5' regions of a human cDNA clone. Southern blot analysis of a panel of human-rodent somatic cell hybrid DNAs revealed that the gene was localized to the short arm of chromosome X, and most probably the pseudoautosomal region of the human X (Xp22.3) and Y (Yp11.3) chromosomes. Several multiallelic restriction fragment length polymorphisms were detected at this locus allowing further localization of the gene by two-point and multipoint linkage analysis in the 40 CEPH families. These results confirmed the pseudoautosomal localization of the HIOMT gene and allowed ordering of the gene in close proximity to DXYS17 at a position about 600-800 kb from the pseudoautosomal boundary and about 1800 to 2000 kb from the telomere. It will be possible to readily identify or exclude the involvement of this gene in genetic diseases by linkage analysis of the disease locus with the high frequency multiallelic polymorphisms at this locus. C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. NR 47 TC 24 Z9 24 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD FEB PY 1993 VL 2 IS 2 BP 127 EP 131 DI 10.1093/hmg/2.2.127 PG 5 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA KM981 UT WOS:A1993KM98100007 PM 8098975 ER PT J AU LAW, SK NUNEZ, PL WIJESINGHE, RS AF LAW, SK NUNEZ, PL WIJESINGHE, RS TI HIGH-RESOLUTION EEG USING SPLINE GENERATED SURFACE LAPLACIANS ON SPHERICAL AND ELLIPSOIDAL SURFACES SO IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING LA English DT Article ID POTENTIAL DATA; HUMAN SCALP; INTERPOLATION; CARTOGRAPHY; TOPOGRAPHY; BRAIN AB Spline generated surface Laplacians are introduced as an effective method for estimating neocortical source activity at moderate scales. The method appears to be robust to the unavoidable perturbations of measured potentials and errors of head geometry and resistivity that are certain to occur in clinical or research settings. In particular, we have derived the surface Laplacian for general ellipsoidal surfaces in terms of the spline function. The spline-Laplacian accurately estimates isolated dipoles or distributed sources, is insensitive to subcortical sources and to sources which originate outside the boundaries of the electrode array, and acts as a bandpass spatial filter whose characteristics appear to provide a good match to the volume conduction of intracranial sources through human heads. As a result, spatial resolution is improved over that obtained with conventional EEG by at least a factor of three. This improvement, whether obtained with spline-Laplacian or model-dependent methods, is likely to have a significant impact on both medical and cognitive studies involving EEG. C1 TULANE UNIV,DEPT BIOMED ENGN,BRAIN PHYS GRP,NEW ORLEANS,LA 70118. RP LAW, SK (reprint author), NIAAA,CLIN STUDIES LAB,BRAIN ELECTROPHYSIOL & IMAGING SECT,BETHESDA,MD 20892, USA. FU NINDS NIH HHS [R01 NS243314] NR 39 TC 115 Z9 115 U1 0 U2 1 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI NEW YORK PA 345 E 47TH ST, NEW YORK, NY 10017-2394 SN 0018-9294 J9 IEEE T BIO-MED ENG JI IEEE Trans. Biomed. Eng. PD FEB PY 1993 VL 40 IS 2 BP 145 EP 153 DI 10.1109/10.212068 PG 9 WC Engineering, Biomedical SC Engineering GA LB759 UT WOS:A1993LB75900005 PM 8319965 ER PT J AU UNSER, M ALDROUBI, A EDEN, M AF UNSER, M ALDROUBI, A EDEN, M TI B-SPLINE SIGNAL-PROCESSING .1. THEORY SO IEEE TRANSACTIONS ON SIGNAL PROCESSING LA English DT Article ID EDGE-DETECTION; INTERPOLATION; REPRESENTATION; RECONSTRUCTION; DERIVATIVES; VISION AB This paper describes a set of efficient filtering techniques for the processing and representation of signals in terms of continuous B-spline basis functions. We first consider the problem of determining the spline coefficients for an exact signal interpolation (direct B-spline transform). The reverse operation is the signal reconstruction from its spline coefficients with an optional zooming factor m (indirect B-spline transform). We derive general expressions for the z transforms and the equivalent continuous impulse responses of B-spline interpolators of order n. We present simple techniques for signal differentiation and filtering in the transformed domain. We then derive recursive filters that efficiently solve the problems of smoothing spline and least squares approximations. The smoothing spline technique approximates a signal with a complete set of coefficients subject to certain regularization or smoothness constraints. The least squares approach, on the other hand, uses a reduced number of B-spline coefficients with equally spaced nodes; this technique is in many ways analogous to the application of antialiasing low-pass filter prior to decimation in order to represent a signal correctly with a reduced number of samples. RP UNSER, M (reprint author), NIH,BIOMED ENGN & INSTRUMENT PROGRAM,BETHESDA,MD 20892, USA. RI Unser, Michael/A-1550-2008; Aldroubi, Akram/J-7186-2012 NR 35 TC 587 Z9 622 U1 4 U2 21 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI NEW YORK PA 345 E 47TH ST, NEW YORK, NY 10017-2394 SN 1053-587X J9 IEEE T SIGNAL PROCES JI IEEE Trans. Signal Process. PD FEB PY 1993 VL 41 IS 2 BP 821 EP 833 DI 10.1109/78.193220 PG 13 WC Engineering, Electrical & Electronic SC Engineering GA KL823 UT WOS:A1993KL82300026 ER PT J AU UNSER, M ALDROUBI, A EDEN, M AF UNSER, M ALDROUBI, A EDEN, M TI B-SPLINE SIGNAL-PROCESSING .2. EFFICIENT DESIGN AND APPLICATIONS SO IEEE TRANSACTIONS ON SIGNAL PROCESSING LA English DT Article ID EDGE-DETECTION; INTERPOLATION; RECONSTRUCTION AB This paper describes a class of recursive filtering algorithms for the efficient implementation of B-spline interpolation and approximation techniques. In terms of simplicity of realization and reduction of computational complexity, these algorithms compare favorably with conventional matrix approaches. A filtering interpretation (low-pass filter followed by an exact polynomial spline interpolator) of smoothing spline and least squares approximation methods is proposed. These techniques are applied to the design of digital filters for cubic spline signal processing. An efficient implementation of a smoothing spline edge detector is proposed. It is also shown how to construct a cubic spline image pyramid that minimizes the loss of information in passage from one resolution level to the next. In terms of common measures of fidelity (e.g., visual quality, SNR), this data structure appears to be superior to the widely used Gaussian/Laplacian pyramid. RP UNSER, M (reprint author), NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892, USA. RI Unser, Michael/A-1550-2008; Aldroubi, Akram/J-7186-2012 NR 39 TC 435 Z9 457 U1 4 U2 25 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI NEW YORK PA 345 E 47TH ST, NEW YORK, NY 10017-2394 SN 1053-587X J9 IEEE T SIGNAL PROCES JI IEEE Trans. Signal Process. PD FEB PY 1993 VL 41 IS 2 BP 834 EP 848 DI 10.1109/78.193221 PG 15 WC Engineering, Electrical & Electronic SC Engineering GA KL823 UT WOS:A1993KL82300027 ER PT J AU HENDERSON, TJ RUDIKOFF, S AF HENDERSON, TJ RUDIKOFF, S TI CHARACTERIZATION OF A VK FAMILY IN MUS-MUSCULUS-CASTANEUS - EXPANSION AT THE SUBSET LEVEL SO IMMUNOGENETICS LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; IMMUNOGLOBULIN HEAVY-CHAIN; VARIABLE-REGION GENES; MULTIGENE FAMILIES; MITOCHONDRIAL-DNA; MYELOMA PROTEINS; ACID-SEQUENCE; GERM-LINE; GENUS MUS; WILD MICE AB We have examined the same kappa chain variable (V(kappa)) region family in several mouse species in order to observe short-term, incremental change at immunoglobulin (Ig) multigene loci. In the present study, the Igk-V24 family has been characterized in a Mus m. castaneus colony derived from individuals originating in Thailand and compared to the same family in Mus m. domesticus (BALB/c) and Mus pahari, representing about 1-2 and about 5-9 million years of evolution, respectively. Southem hybridization of genomic DNA with a probe encoding the prototype Igk-V24 coding region reveals restriction fragment patterns indicative of two distinct M. m. castaneus haplotypes. These haplotypes appear to result from an unequal recombination between similar gene arrays, as their restriction patterns are unique but contain many common fragments. The complexity of these patterns indicates a marked expansion in the Igk-V24 family of M. m. castaneus relative to BALB/c and M. pahari. Additional analyses using probes specific for individual subsets demonstrate that the expansion is not general throughout the entire family, but is restricted to particular subsets and therefore to relatively short chromosomal segments. One subset alone accounts for most of the expansion and comprises over 40% of the entire M. m. castaneus family. The wide range of Igk-V24 family complexity seen among M. m. castaneus, M. m. domesticus, and M. pahari, as well as among the different M. m. castaneus family subsets, suggests a model of random evolution in V(kappa) family copy number rather than one which is selective. C1 NCI,GENET LAB,BETHESDA,MD 20892. UNIV MARYLAND,DEPT CHEM & BIOCHEM,COLL PK,MD 20742. NR 38 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PD FEB PY 1993 VL 37 IS 6 BP 415 EP 425 PG 11 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA KN420 UT WOS:A1993KN42000003 PM 8436416 ER PT J AU HENDERSON, TJ RUDIKOFF, S AF HENDERSON, TJ RUDIKOFF, S TI CHARACTERIZATION OF A VK FAMILY IN MUS-MUSCULUS-CASTANEUS - SEQUENCE-ANALYSIS SO IMMUNOGENETICS LA English DT Article ID IMMUNOGLOBULIN HEAVY-CHAIN; VARIABLE-REGION GENES; WILD MICE; SOMATIC MUTATION; GERM-LINE; NUCLEOTIDE-SEQUENCES; RECOMBINATION SITES; CONSERVED SEQUENCE; EVOLUTION; DNA AB To examine genetic variation at immunoglobulin (Ig) multigene loci over short spans of evolutionary time, we have compared members of an Ig kappa chain variable (Vkappa) region family from several mouse species. In this study, seven unique Igk-V24 family members have been isolated from Mus m. castaneus and characterized by nucleotide sequence determination for comparison to their counterparts in Mus m. domesticus (BALB/c), and Mus pahari, representing 1-2 million years of evolution in the former case and 5-8 million years in the latter. Parsimony, together with evolutionary distances calculated for various pairs of Igk-V24 family coding regions, relate all family members to a common progenitor existing roughly 24 million years ago (Mya). A significant portion of the M. m. castaneus family consists of pseudogene segments in various degrees of progressive degeneration. The substitution patterns and divergence rates for all gene segments are characteristic of their respective subsets, especially in the areas flanking the coding regions. Complex and variable patterns of diversity are seen in potentially expressed coding regions, which appear to reflect quite different selective pressures on various subregions within the Vkappa protein domain. These results indicate that evolutionary pressures are operating at the level of family subsets, their individual members, and subregions within similar molecules. C1 NCI,GENET LAB,BETHESDA,MD 20892. UNIV MARYLAND,DEPT CHEM & BIOCHEM,COLL PK,MD 20742. NR 51 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PD FEB PY 1993 VL 37 IS 6 BP 426 EP 436 PG 11 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA KN420 UT WOS:A1993KN42000004 PM 8436417 ER PT J AU CORRIVEAU, CC DANNER, RL AF CORRIVEAU, CC DANNER, RL TI ENDOTOXIN AS A THERAPEUTIC TARGET IN SEPTIC SHOCK SO INFECTIOUS AGENTS AND DISEASE-REVIEWS ISSUES AND COMMENTARY LA English DT Article DE ENDOTOXIN; SEPTIC SHOCK ID TUMOR-NECROSIS-FACTOR; LIPOPOLYSACCHARIDE-BINDING-PROTEINS; GRAM-NEGATIVE ENDOTOXIN; ESCHERICHIA-COLI; LIPID-A; CLINICAL-SIGNIFICANCE; CANINE MODEL; BACTERIAL LIPOPOLYSACCHARIDES; CARDIOVASCULAR DYSFUNCTION; MURINE SPLENOCYTES AB Endotoxin initiates a broad inflammatory response in mammals that has many similarities to de novo septic shock in patients. The presumed role of endotoxin in septic shock has led to the investigation of antiendotoxin therapies for the treatment of this syndrome. However, a causative role for endotoxin in human septic shock has not been established. Recent information about the biochemistry of endotoxin, mechanisms of endotoxin-induced cell activation, and the pathogenesis of septic shock has provided insights useful to the development of new antiendotoxin agents, but also has raised concerns about the suitability of endotoxin as a therapeutic target. Definitive proof of the pathogenic importance of endotoxin in human septic shock will depend upon demonstrating that a putative antiendotoxin therapy has clinical efficacy. C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT CRIT CARE MED,BLDG 10,BETHESDA,MD 20892. CHILDRENS NATL MED CTR,DEPT CRIT CARE,WASHINGTON,DC. NR 83 TC 38 Z9 39 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1056-2044 J9 INFECT AGENT DIS JI Infect. Agents Dis.-Rev. Issues Comment. PD FEB PY 1993 VL 2 IS 1 BP 35 EP 43 PG 9 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA KV874 UT WOS:A1993KV87400004 PM 8162352 ER PT J AU CORRIVEAU, CC DANNER, RL AF CORRIVEAU, CC DANNER, RL TI ANTIENDOTOXIN THERAPIES FOR SEPTIC SHOCK SO INFECTIOUS AGENTS AND DISEASE-REVIEWS ISSUES AND COMMENTARY LA English DT Article DE ENDOTOXIN; SEPTIC SHOCK ID GRAM-NEGATIVE BACTEREMIA; POLYMYXIN-B SULFATE; PERMEABILITY-INCREASING PROTEIN; MONOCLONAL IGM ANTIBODY; ESCHERICHIA-COLI J5; SYNTHETIC LIPID-X; BACTERIAL LIPOPOLYSACCHARIDES; CROSS-REACTIVITY; INTRAVASCULAR COAGULATION; DENSITY LIPOPROTEINS AB Gram-negative shock is thought to result primarily from the effects of endotoxin, a component of the bacterial outer membrane. Accordingly, therapies aimed at inhibiting, neutralizing, or clearing endotoxin have been extensively explored. Despite over 30 years of research, no antiendotoxin approach to the treatment of human septic shock is of proven benefit. In recent randomized clinical trials of monoclonal antibodies against endotoxin, therapeutic efficacy was not convincingly demonstrated. This result, however, does not eliminate the possibility that other antiendotoxin therapies may be effective. The antibodies used in these clinical trials do not appear to neutralize endotoxin in vitro and are not reproducibly protective in animal models of sepsis. Newer agents with well-defined mechanisms of antiendotoxin activity may help clarify the role of endotoxin in septic shock and prove useful therapy for some patients. C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT CRIT CARE MED,BLDG 10,RM 7043,BETHESDA,MD 20892. CHILDRENS NATL MED CTR,DEPT CRIT CARE,WASHINGTON,DC. NR 95 TC 13 Z9 16 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1056-2044 J9 INFECT AGENT DIS JI Infect. Agents Dis.-Rev. Issues Comment. PD FEB PY 1993 VL 2 IS 1 BP 44 EP 52 PG 9 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA KV874 UT WOS:A1993KV87400005 PM 8162353 ER PT J AU HUPPI, K SIWARSKI, D SHAUGHNESSY, JD MUSHINSKI, JF AF HUPPI, K SIWARSKI, D SHAUGHNESSY, JD MUSHINSKI, JF TI CO-AMPLIFICATION OF C-MYC/PVT-1 IN IMMORTALIZED MOUSE B-LYMPHOCYTIC CELL-LINES RESULTS IN A NOVEL PVT-1/AJ-1 TRANSCRIPT SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID TUMOR-CELLS; MYC; TRANSLOCATIONS; GENE; PVT; PLASMACYTOMAS; CHROMOSOME-15; ONCOGENE; REGION AB In a series of mouse pvt-I cDNA clones prepared from an immortalized B-cell line that contains an amplified c-myc/pvt-I region, we have identified a unique cDNA, AJ-I, which contains 57 bp of pvt-I sequence (pvt-I a) spliced to a novel sequence of 2.1 kb. We report here that this 3' segment (termed AJ-IX) maps more than 60 kb telomeric to pvt-I a and is encoded by a novel locus (Aj-I) on mouse chromosome 15. The AJ-IX probe detects a transcript that is expressed only in normal mouse brain and testis. Several mast-cell tumors, Ly-I+ B-lymphocytic cell lines and a neuroblastoma also display abundant levels of AJ-IX-specific mRNA. However, splicing of pvt-Ia to AJ-IX is found exclusively in Ly-I+ B-cell lines that contain amplified c-myc/pvt-I. We conclude that some feature in the generation of this amplicon facilitates the synthesis of a pvt-I/AJ-IX chimeric mRNA that may play a role in immortalization of these Ly-I+ B-cell lines. RP HUPPI, K (reprint author), NCI,GENET LAB,MOLEC GENET SECT,BLDG 37,ROOM 2B-21,BETHESDA,MD 20892, USA. NR 19 TC 8 Z9 8 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD FEB 1 PY 1993 VL 53 IS 3 BP 493 EP 498 DI 10.1002/ijc.2910530323 PG 6 WC Oncology SC Oncology GA KK633 UT WOS:A1993KK63300022 PM 8094075 ER PT J AU KALAN, JM GERTZ, SD KRAGEL, AH BERGER, PB ROBERTS, WC RYAN, TJ AF KALAN, JM GERTZ, SD KRAGEL, AH BERGER, PB ROBERTS, WC RYAN, TJ TI EFFECTS OF TISSUE PLASMINOGEN-ACTIVATOR THERAPY ON THE FREQUENCY OF ACUTE RIGHT VENTRICULAR MYOCARDIAL-INFARCTION ASSOCIATED WITH ACUTE LEFT-VENTRICULAR INFARCTION SO INTERNATIONAL JOURNAL OF CARDIOLOGY LA English DT Article DE RIGHT VENTRICULAR INFARCTION; THROMBOLYSIS; REPERFUSION; CORONARY ARTERY DISEASE ID CORONARY HEART-DISEASE; CREATINE-KINASE; ARTERY; SIZE; REPERFUSION AB To assess the effects of reperfusion therapy on acute right ventricular myocardial infarction, we studied at necropsy the hearts from 51 patients who died after receiving intravenous recombinant tissue plasminogen activator for acute left ventricular myocardial infarction as part of the Thrombolysis in Myocardial Infarction (TIMI) study. Right ventricular infarction occurred in none of 29 patients with infarction of the anterior wall of the left ventricle and in 8 of 22 patients (36%) with infarction of the posterior (inferior) wall of the left ventricle. Of the 22 patients with posterior wall infarction, the 8 patients with right ventricular infarction were compared to the 14 patients without right ventricular infarction. The patients with right ventricular infarction had a longer mean interval from tissue plasminogen activator infusion to peak creatine phosphokinase level (19 vs. 11 h, P < 0.03), a lower frequency of hemorrhagic necrosis (2 of 8 vs. 10 of 14, P < 0.04) and higher frequency of luminal thrombus in the infarct-related coronary artery (6 of 8 vs. 3 of 14, P = 0.054). Each of these findings is associated with the absence of coronary reperfusion. Thus, successful reperfusion following acute left ventricular myocardial infarction appears to be associated with a decreased frequency of concomitant right ventricular myocardial infarction. C1 NHLBI,PATHOL BRANCH,BLDG 10,ROOM 2N258,BETHESDA,MD 20892. HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT ANAT & EMBRYOL,IL-91010 JERUSALEM,ISRAEL. BOSTON UNIV HOSP,MED CTR,CARDIOL SECT,BOSTON,MA 02218. NR 18 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0167-5273 J9 INT J CARDIOL JI Int. J. Cardiol. PD FEB PY 1993 VL 38 IS 2 BP 151 EP 158 DI 10.1016/0167-5273(93)90174-F PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA KP213 UT WOS:A1993KP21300006 PM 8454377 ER PT J AU KITTUR, SD ADLER, WH MARTIN, GR SCHAPIRO, MB RAPOPORT, SI GUNZLER, V AF KITTUR, SD ADLER, WH MARTIN, GR SCHAPIRO, MB RAPOPORT, SI GUNZLER, V TI LAMININ CONCENTRATIONS IN SERUM AND CEREBROSPINAL-FLUID IN AGING AND ALZHEIMERS-DISEASE SO INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE LA English DT Article DE DEMENTIA; EXTRACELLULAR MATRIX; LAMININ ID NEURONS; PROTEIN AB Laminin, a basement membrane protein, and a potent promoter of neurite outgrowth, surrounds all peripheral nerves. It appears in the central nervous system during development and reappears in response to injury. In Alzheimer's disease (AD), there is a progressive loss of neurons in specific areas of the brain along with the presence of an increased number of senile plaques and neurofibrillary tangles. Laminin levels have been shown to be increased in injury, so we undertook to examine levels of laminin by radioimmunoassay (RIA), in cerebrospinal fluid and serum of patients with AD and age-matched controls. No difference in the CSF and serum laminin concentrations was found between Alzheimer's disease and age-matched controls. We found a lack of correlation between severity of clinical dementia and laminin concentrations. Finally, we show that the CSF and serum laminin concentrations increase with age. C1 NIA,NEUROSCI LAB,BALTIMORE,MD 21224. HOECHST AG,W-6230 FRANKFURT 80,GERMANY. RP KITTUR, SD (reprint author), NIA,GRC,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 20 TC 6 Z9 6 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0736-5748 J9 INT J DEV NEUROSCI JI Int. J. Dev. Neurosci. PD FEB PY 1993 VL 11 IS 1 BP 95 EP 99 DI 10.1016/0736-5748(93)90038-F PG 5 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA KV641 UT WOS:A1993KV64100010 PM 8488758 ER EF