FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Leibovitch, BA Campbell, DB Krishnan, KS Nash, HA AF Leibovitch, BA Campbell, DB Krishnan, KS Nash, HA TI Mutations that affect ion channels change the sensitivity of Drosophila melanogaster to volatile anesthetics SO JOURNAL OF NEUROGENETICS LA English DT Article DE general anesthesia; inebriometer; halothane; shaker; paralytic; slowpoke; har mutants ID POTASSIUM CHANNELS; MUTANT; HALOTHANE; GENE AB We have quantitated the response of D. melanogaster to general anesthetics with a device, the inebriometer, that assays the fly's geotactic and postural behavior. When alleles of several loci that encode or regulate subunits of ion channels were compared with control stocks, several ion channel mutants clearly increased the anesthetic sensitivity of Drosophila. The effects were specific in that: a) for several alleles, genetic tests indicated that the anesthesia phenotype was due to the ion channel mutation and not to extraneous genetic differences between the stocks; b) a given ion channel mutation often affected the response to one anesthetic but not another; and c) the behavior of decapitated flies in the inebriometer indicated that the anesthetic phenotype of several mutants did not merely reflect a global change in the fly's physiology. These results provide support for the idea that ion channels are on the pathway(s) influenced by anesthetics and that different anesthetics use different pathways. They also provide perspective on the behavior of previously isolated mutations (har) that decrease the sensitivity of Drosophila to anesthetics in the inebriometer. C1 NIMH,MOLEC BIOL LAB,BETHESDA,MD 20892. NR 25 TC 31 Z9 33 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0167-7063 J9 J NEUROGENET JI J. Neurogenet. PY 1995 VL 10 IS 1 BP 1 EP 13 DI 10.3109/01677069509083455 PG 13 WC Genetics & Heredity; Neurosciences SC Genetics & Heredity; Neurosciences & Neurology GA UC107 UT WOS:A1995UC10700001 PM 8618174 ER PT J AU AZARI, NP PIETRINI, P AF AZARI, NP PIETRINI, P TI PRECLINICAL STAGES IN SUBJECTS AT RISK FOR NEUROLOGICAL DISORDERS - CAN PET-FDG TELL US MORE SO JOURNAL OF NEUROLOGY LA English DT Letter ID EMISSION TOMOGRAPHIC DATA; ALZHEIMERS-DISEASE; PATTERN; CHOREA C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 11 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-5354 J9 J NEUROL JI J. Neurol. PD JAN PY 1995 VL 242 IS 2 BP 112 EP 114 DI 10.1007/BF00887827 PG 3 WC Clinical Neurology SC Neurosciences & Neurology GA QD239 UT WOS:A1995QD23900013 PM 7707087 ER PT J AU GAJDUSEK, DC AF GAJDUSEK, DC TI THE BIOHAZARD OF NUCLEATING INDUCTION OF INFECTIOUS AMYLOIDS FROM HOST PRECURSORS SO JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY LA English DT Article; Proceedings Paper CT Symposium on Brain Research and Society CY SEP 21-24, 1995 CL BARCELONA, SPAIN RP GAJDUSEK, DC (reprint author), NIH,BLDG 10,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSN NEUROPATHOLOGISTS INC PI LAWRENCE PA 1041 NEW HAMPSHIRE ST, LAWRENCE, KS 66044 SN 0022-3069 J9 J NEUROPATH EXP NEUR JI J. Neuropathol. Exp. Neurol. PY 1995 VL 54 SU S BP S12 EP S12 PG 1 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA RU716 UT WOS:A1995RU71600007 ER PT J AU VILNER, BJ DECOSTA, BR BOWEN, WD AF VILNER, BJ DECOSTA, BR BOWEN, WD TI CYTOTOXIC EFFECTS OF SIGMA-LIGANDS - SIGMA-RECEPTOR MEDIATED ALTERATIONS IN CELLULAR MORPHOLOGY AND VIABILITY SO JOURNAL OF NEUROSCIENCE LA English DT Article DE SIGMA RECEPTORS; NEUROLEPTICS; CYTOTOXICITY; NEURODEGENERATION; C6 GLIOMA; HALOPERIDOL ID GUINEA-PIG BRAIN; CYCLIC NUCLEOTIDE PHOSPHODIESTERASE; CALCIUM-DEPENDENT ACTIVATOR; BINDING-SITES; H-3 (+)-PENTAZOCINE; HIGH-AFFINITY; GLUTAMATE NEUROTOXICITY; BIOLOGICAL EVALUATION; GLUCOSE DEPRIVATION; HIPPOCAMPAL-NEURONS AB The morphological effects of several neuroleptics as well as other novel and prototypic sigma ligands were examined by addition to cultures of C6 glioma cells. Sigma ligands caused loss of processes, assumption of spherical shape, and cessation of cell division. The time course and magnitude of this effect were dependent on the concentration of sigma ligand. Continued exposure to sigma compounds ultimately resulted in cell death. However, the morphological effect was reversible when sigma ligand was removed shortly after rounding. The potency of compounds to produce these effects generally correlated with binding affinity at sigma receptors of C6 glioma cell membranes labeled with [H-3](+)pentazocine. At a concentration of 100 mu M, haloperidol, reduced haloperidol, fluphenazine, perphenazine, trifluoperazine, BD737, LR172, BD1008, and SH344 produced significant effects in 3-6 hr of exposure. Other compounds, such as trifluperidol, thioridazine, and (-)-butaclamol, produced significant effects by 24 hr of exposure. Despite the requirement of micromolar concentrations of ligand (some compounds were effective at 30 mu M), the effect showed a remarkable specificity for compounds exhibiting sigma receptor binding affinity. Neuroleptics lacking potent sigma affinity [e.g., (-)-sulpiride, (+)-butaclamol, and clozapine] and other compounds that lack significant sigma affinity but that are agonists or antagonists at dopamine, serotonin, adrenergic, glutamate, phencyclidine, GABA, opiate, or muscarinic cholinergic receptors were without effect on cellular morphology at concentrations up to 300 mu M over a period of 72 hr. Likewise, blockers and activators of Na+, K+, and Ca2+ channels and a monoamine oxidase inhibitor devoid of sigma affinity were without effect. Interestingly, 1,3-di-o-tolylguanidine (DTG), (+)-3-(3-hydroxyphenyl)-N-(1-propyl)piperidine [(+)-3-PPP], (+)-pentazocine, (+)-cyclazocine, and other sigma-active benzomorphans and morphinans appeared inactive in up to 72 hr of culture. However, these compounds interacted synergistically with a subeffective dose of BD737 (30 mu M) to produce effects usually in 6 hr or less. Also, the pH of the culture medium had a profound effect on the activity of sigma compounds. Increasing the pH from the normal range of 7.2-7.4 to pH 8.3-8.5 shifted the dose curves (30, 100, 300 mu M) for all sigma compounds to the left. Under these conditions, DTG, (+)-3-PPP, and benzomorphans produced effects in 24 hr or less. Decreasing the medium pH to 6.5-6.7 markedly reduced the activity of all sigma ligands, producing significant protection from cytotoxic effects. Importantly, compounds that lacked sigma binding affinity showed neither synergism with 30 mu M BD737 nor an increase in activity at higher pH. These results confirm the sigma receptor specificity of this effect. Sigma ligands had similar effects on other cells of neuronal and non-neuronal origin, including SK-N-SH and SH-SY5Y neuroblastomas, NCB-20 hybridoma, NG108-15 neuroblastoma-glioma hybrid, COS-7 (kidney), MRS-5 (lung), and PC12 pheochromocytoma. These results suggest that sigma receptors play some vital role in cell function and may have important implications for neurodegenerative disorders and neuroleptic treatment. C1 NIDDK,MED CHEM LAB,RECEPTOR BIOCHEM & PHARMACOL UNIT,BETHESDA,MD 20892. NR 89 TC 135 Z9 136 U1 1 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN PY 1995 VL 15 IS 1 BP 117 EP 134 PN 1 PG 18 WC Neurosciences SC Neurosciences & Neurology GA QB811 UT WOS:A1995QB81100010 PM 7823122 ER PT J AU ANTONIJEVIC, I MOUSA, SA SCHAFER, M STEIN, C AF ANTONIJEVIC, I MOUSA, SA SCHAFER, M STEIN, C TI PERINEURIAL DEFECT AND PERIPHERAL OPIOID ANALGESIA IN INFLAMMATION SO JOURNAL OF NEUROSCIENCE LA English DT Article DE PAIN; OPIOIDS; LOCAL ANALGESIA; ANTINOCICEPTION; INFLAMMATION; PERINEURIUM; BLOOD-NERVE BARRIER; PEPTIDES; HORSERADISH PEROXIDASE; HYPEROSMOLAR SOLUTIONS; MANNITOL; LIPOPHILIC COMPOUNDS; FENTANYL ID BLOOD-BRAIN-BARRIER; SPINAL-CORD; HORSERADISH-PEROXIDASE; INFLAMED TISSUE; AXONAL-TRANSPORT; RECEPTOR-BINDING; GENE-EXPRESSION; NERVE; RAT; ANTINOCICEPTION AB Opioid receptors have been demonstrated on sensory nerves in both inflamed and normal subcutaneous tissue but locally applied opioid agonists produce analgesia in inflamed tissue only. Inflammation confers a disruption of the perineurial barrier that can also be induced deliberately by hyperosmolar solutions, The present study examines at which stage of Freund's adjuvant-induced inflammation peripheral opioid analgesic effects become manifest and whether a perineurial defect contributes to the appearance of such effects. To this end we have monitored the temporal evolution of inflammatory signs (swelling, temperature, hyperalgesia) and of peripheral antinociceptive effects (by the paw pressure test) of mu-, delta-, and kappa-selective opioids, Using horseradish peroxidase histochemistry, the perineurial barrier was assessed in normal and inflamed tissue and following its artificial disruption by hyperosmolar saline and mannitol in vivo. Finally, we sought to elicit analgesia in normal tissue by the concomitant application of mannitol and receptor-selective opioids or by an extremely lipophilic opioid agonist (fentanyl). We found that peripheral opioid antinociception and perineurial leakage occur simultaneously at a very early stage (within 12 hr) of the inflammatory reaction and that both can be mimicked by the administration of hyperosmolar solutions in normal tissue, Fentanyl produced peripheral antinociception in noninflamed tissue that was potentiated by mannitol or inflammation. Our findings demonstrate that the perineurium is a crucial determinant for peripheral opioid analgesia and that the efficacy of locally applied hydrophilic or lipophilic neuromodulatory compounds can be improved dramatically by the concomitant modulation of perineurial permeability. Furthermore, these observations indicate an unrestricted transperineurial passage for peptides in inflammation and thus add a further integral component to our previously outlined concept of a direct communication between immune cell-derived endogenous opioid peptides and sensory nerves resulting in the inhibition of inflammatory pain. C1 AFRC,BABRAHAM INST,DEPT NEUROBIOL,CAMBRIDGE CB2 4AT,ENGLAND. NIDA,ADDICT RES CTR,BEHAV PHARMACOL & GENET SECT,BALTIMORE,MD 21224. JOHNS HOPKINS UNIV,SCH MED,DEPT ANESTHESIOL & CRIT CARE MED,BALTIMORE,MD 21287. OI Stein, Christoph/0000-0001-5240-6836 FU NINDS NIH HHS [R01 NS32466] NR 44 TC 210 Z9 219 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN PY 1995 VL 15 IS 1 BP 165 EP 172 PN 1 PG 8 WC Neurosciences SC Neurosciences & Neurology GA QB811 UT WOS:A1995QB81100014 PM 7823127 ER PT J AU LI, AR LANE, WS JOHNSON, LV CHADER, GJ TOMBRANTINK, J AF LI, AR LANE, WS JOHNSON, LV CHADER, GJ TOMBRANTINK, J TI NEURON-SPECIFIC ENOLASE - A NEURONAL SURVIVAL FACTOR IN THE RETINAL EXTRACELLULAR-MATRIX SO JOURNAL OF NEUROSCIENCE LA English DT Article DE NEURON-SPECIFIC ENOLASE; RETINA; NEURON SURVIVAL FACTOR; INTERPHOTORECEPTOR MATRIX; EXTRACELLULAR MATRIX; PHOTORECEPTOR; NEURON; NEURONOTROPHIC PROTEIN ID EPITHELIUM-DERIVED FACTOR; AMINO-ACID-SEQUENCE; INTERPHOTORECEPTOR MATRIX; NEUROTROPHIC ACTIVITY; RETINOBLASTOMA CELLS; BINDING-PROTEIN; DIFFERENTIATION; BRAIN AB To identify soluble proteins of the retinal interphotoreceptor matrix (IPM), we isolated IPM from the bovine eye by gentle ravage and subjected it to SDS-PAGE, In the resultant gel, a 46 kDa band was particularly prominent and appeared to be a single protein, This protein was electroblotted to nitrocellulose membrane, digested with trypsin, and selected peptides were isolated by HPLC and subjected to Edman microsequencing. The amino acid sequences of the peptides were found to be virtually identical to that of human neuron-specific enolase (NSE), A monoclonal antibody specific for human NSE confirmed the presence of this enzyme in the bovine IPM by both Western blotting and immunocytochemical analysis, Immunofluorescence microscopy demonstrated that NSE is mainly localized to the basal domain of the IPM surrounding photoreceptor cells but is also prominent in the inner segments of the cone photoreceptor neurons, When NSE was added to cultures of human retinoblastoma cells, no effect on morphology was observed, However, a positive effect on cell growth and/or survival was readily apparent, It thus seems that not only is NSE a significant component of the retinal extracellular matrix, but that it could function as a survival (neuronotrophic) factor for photoreceptor neurons. C1 NEI,RETINAL CELL & MOLEC BIOL LAB,BETHESDA,MD 20892. HARVARD UNIV,HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138. UNIV SO CALIF,SCH MED,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90033. NR 22 TC 25 Z9 25 U1 0 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN PY 1995 VL 15 IS 1 BP 385 EP 393 PN 1 PG 9 WC Neurosciences SC Neurosciences & Neurology GA QB811 UT WOS:A1995QB81100033 PM 7823143 ER PT J AU GALLO, V ARMSTRONG, RC AF GALLO, V ARMSTRONG, RC TI DEVELOPMENTAL AND GROWTH FACTOR-INDUCED REGULATION OF NESTIN IN OLIGODENDROCYTE LINEAGE CELLS SO JOURNAL OF NEUROSCIENCE LA English DT Article DE O-2A PROGENITORS; INTERMEDIATE FILAMENTS; CG-4 CELLS; PLATELET-DERIVED GROWTH FACTOR; BASIC FIBROBLAST GROWTH FACTOR; B104 NEUROBLASTOMA ID CENTRAL-NERVOUS-SYSTEM; MONOCLONAL-ANTIBODIES O1; NEURONAL PRECURSOR CELLS; GLIAL PROGENITOR-CELL; RAT CEREBELLUM; TYPE-2 ASTROCYTES; MACROGLIAL CELLS; PRIMARY CULTURE; DIFFERENTIATION; EXPRESSION AB The expression and regulation of nestin, an intermediate filament protein associated with neuroepithelium-derived progenitor cells, was studied in developing oligodendrocytes. We analyzed glial cells cultured from late embryonic rat cerebral cortex, tissue prints from neonatal rat brains, and the oligodendrocyte cell line CG-4. Using cortical- or CG-4-derived oligodendrocyte lineage cells, Northern blot analysis demonstrated that nestin mRNA was highly expressed in proliferating O-2A progenitors, but downregulated in differentiated oligodendrocytes. Immunocytochemistry of cultured cells and tissue prints demonstrated that pre-O-2A cells and O-2A progenitors expressed high levels of the nestin protein. In contrast, nestin expression decreased during the pro-oligodendroblast stage, and the majority of oligodendrocytes were not labeled with anti-nestin antibodies. Culture conditions (PDGF + bFGF or B104-conditioned medium) that maintained O-2A progenitor proliferation and prevented differentiation enhanced expression of nestin mRNA transcripts and protein. In comparison, analysis of cortical astrocyte cultures demonstrated that type 1 astrocytes, but not type 2, expressed nestin mRNA and protein. Our findings demonstrate that nestin is expressed in developing glial cells, which is suggestive that this intermediate filament protein may play an important role during gliogenesis. C1 UNIFORMED SERV UNIV HLTH SCI,DEPT ANAT & CELL BIOL,BETHESDA,MD 20814. RP GALLO, V (reprint author), NICHHD,CELLULAR & MOLEC NEUROPHYSIOL LAB,BLDG 49,ROOM 5A78,BETHESDA,MD 20892, USA. NR 69 TC 118 Z9 118 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN PY 1995 VL 15 IS 1 BP 394 EP 406 PN 1 PG 13 WC Neurosciences SC Neurosciences & Neurology GA QB811 UT WOS:A1995QB81100034 PM 7823144 ER PT J AU LEFEROVICH, JM LANA, DP SUTRAVE, P HUGHES, SH KELLY, AM AF LEFEROVICH, JM LANA, DP SUTRAVE, P HUGHES, SH KELLY, AM TI REGULATION OF C-SKI TRANSGENE EXPRESSION IN DEVELOPING AND MATURE MICE SO JOURNAL OF NEUROSCIENCE LA English DT Article DE C-SKI; TRANSGENE; DEVELOPMENT; HYPERTROPHY; CONTROL; NEURAL ID MOUSE SKELETAL-MUSCLE; MESSENGER-RNA; NERVE INJURY; ONCOGENE; MYOSIN; RAT; SLOW; DIFFERENTIATION; OVEREXPRESSION; VIRUSES AB The control of c-ski transgene expression and muscle hypertrophy have been investigated in transgenic mice. In adult animals, the level of transgene expression is linked to the specialized phenotype of individual muscles, high levels occur in fast muscles and significantly lower levels in muscles with high metabolic activity (diaphragm, soleus). These findings have led us to propose that a threshold must be passed before ski-induced growth can occur. We now show that within fast muscles, induced hypertrophy uniquely involves IIb fibers. This pattern of expression is under development control; levels of c-ski mRNA are low in all muscles at birth. In the diaphragm, there is a sevenfold increase in c-ski message levels between 5 d and maturity. By contrast, in fast extensor digitorum longus and anterior tibial muscles, there is a 24-fold increase in levels between 5 and 12 d postpartum. Muscle hypertrophy and antibody staining for c-ski protein in myofiber nuclei emerge concurrently. This pattern of c-ski expression parallels the appearance of IIb myosin heavy chain transcripts (Wydert et al., 1987) and differentiation of IIb fibers, suggesting that amplification of c-skim RNA levels is linked to the development of IIb fiber specialization. Manipulations that are known to perturb IIb fiber development, neonatal denervation, and neonatally induced hypothyroidism inhibit high levels of c-ski expression and hypertrophy. In the adult fast EDL, denervation leads to rapid atrophy of IIb fibers and a significant decline in levels of c-ski mRNA. The results suggest that the environment of differentiated IIb fibers permits the expression of high levels of c-ski mRNA and this, in turn, induces hypertrophy. C1 UNIV PENN,SCH VET MED,DEPT PATHOBIOL,PHILADELPHIA,PA 19104. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. FU NCI NIH HHS [N01-CO-74101]; NHLBI NIH HHS [HL 15835] NR 25 TC 29 Z9 29 U1 0 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD JAN PY 1995 VL 15 IS 1 BP 596 EP 603 PN 2 PG 8 WC Neurosciences SC Neurosciences & Neurology GA QB812 UT WOS:A1995QB81200012 PM 7823166 ER PT J AU PELTONEN, K DIPPLE, A AF PELTONEN, K DIPPLE, A TI POLYCYCLIC AROMATIC-HYDROCARBONS - CHEMISTRY OF DNA ADDUCT FORMATION SO JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE LA English DT Article ID REGION DIOL-EPOXIDES; MOUSE SKIN; BENZOPYRENE 7,8-DIOL-9,10-EPOXIDES; METABOLIC-ACTIVATION; OPTICAL ENANTIOMERS; DIHYDRODIOL EPOXIDE; NUCLEOSIDE ADDUCTS; CELL-CULTURES; ACID; BINDING AB Polycyclic aromatic hydrocarbon carcinogens are usually activated for DNA binding by the metabolic formation of bay region dihydrodiol epoxides with R,S,S,R stereochemistry. Such metabolites from planar hydrocarbons reacted preferentially with the amino groups of deoxyguanosine residues, whereas those from nonplanar hydrocarbons were more efficiently trapped by DNA and reacted preferentially with the amino groups of deoxyadenosine residues, in some cases. Molecular modeling and NMR measurements indicated that the conformations of DNA adducts depend upon the hydrocarbon involved and the cis or trans opening of the epoxide ring during adduct formation. The structural characterization of carcinogen-DNA adducts and investigations of relationships between specific adducts and biological effects represent an important background that can be valuable in molecular epidemiological approaches. C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,CHEM CARCINOGENESIS LAB,FREDERICK,MD. RP PELTONEN, K (reprint author), INST OCCUPAT HLTH,MOLEC DOSIMETRY GRP,TOPELIUKSENKATU 41 AA,HELSINKI,FINLAND. FU NCI NIH HHS [N01-CO-74101] NR 45 TC 52 Z9 53 U1 0 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1076-2752 J9 J OCCUP ENVIRON MED JI J. Occup. Environ. Med. PD JAN PY 1995 VL 37 IS 1 BP 52 EP 58 DI 10.1097/00043764-199501000-00008 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA QH085 UT WOS:A1995QH08500007 PM 7620943 ER PT J AU MUELLER, BU PIZZO, PA AF MUELLER, BU PIZZO, PA TI CANCER IN CHILDREN WITH PRIMARY OR SECONDARY IMMUNODEFICIENCIES SO JOURNAL OF PEDIATRICS LA English DT Article ID POSTTRANSPLANT LYMPHOPROLIFERATIVE DISORDER; IMMUNE-DEFICIENCY-SYNDROME; ATAXIA-TELANGIECTASIA; VIRUS INFECTION; HIV-INFECTION; TRANSPLANT RECIPIENTS; PEDIATRIC-PATIENTS; MALIGNANT-TUMORS; LYMPHOMA; DISEASE RP MUELLER, BU (reprint author), NCI, PEDIAT BRANCH, BLDG 10, ROOM 13N240, BETHESDA, MD 20892 USA. NR 54 TC 77 Z9 77 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD JAN PY 1995 VL 126 IS 1 BP 1 EP 10 DI 10.1016/S0022-3476(95)70491-4 PG 10 WC Pediatrics SC Pediatrics GA QB184 UT WOS:A1995QB18400001 PM 7815195 ER PT J AU SANNERUD, CA ATOR, NA AF SANNERUD, CA ATOR, NA TI DRUG DISCRIMINATION ANALYSIS OF MIDAZOLAM UNDER A 3-LEVER PROCEDURE .1. DOSE-DEPENDENT DIFFERENCES IN GENERALIZATION AND ANTAGONISM SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID BENZODIAZEPINE RECEPTOR ANTAGONIST; STIMULUS-GENERALIZATION; SALINE DISCRIMINATION; CROSS-GENERALIZATION; TRAINING CONDITIONS; AGONIST PROPERTIES; RO 15-1788; RATS; RO-15-1788; MORPHINE AB Twelve rats were trained to discriminate two doses of midazolam, 0.32 and 3.2 mg/kg, from no drug under a three-lever, multiple-trials procedure (15-min time-out, 5-min fixed-ratio-10 schedule of food reinforcement). In tests, midazolam (0.0032-10 mg/kg), administered cumulatively within a session or acutely across sessions, produced dose-dependent increases in responding on the low-dose lever after 0.1 to 1.0 mg/kg and on the high-dose lever at higher doses in all rats. Flumazenil dose-dependently antagonized the discriminative stimulus effect of 3.2 mg/kg of midazolam in ail rats but antagonism of the lower midazolam training dose was not obtained in an rats. Pentobarbital dose-dependently produced responding on the levers associated with the no-drug and 0.32-mg/kg midazolam conditions but not on the lever associated with 3.2 mg/kg of midazolam. These results differed from those that would have been predicted from studies in midazolam-trained rats in two-lever procedures. The muscle relaxant methocarbamol and nonsedative/anxiolytic drugs (morphine, caffeine and d-amphetamine) did not produce responding on the lever associated with either the low or high midazolam training dose. However, cocaine produced partial responding on the lever associated with the low midazolam dose. Thus, the discriminative stimulus effects of 3.2 mg/kg of midazolam were benzodiazepine-like and not a function of general sedative or muscle-relaxant effects. The 0.32-mg/kg midazolam training dose, in this context, appeared less specific than 3.2 mg/kg of midazolam. Taken together, the results suggest thai not all differences among the training stimuli in this three-lever context reflect simple differences in dose. C1 NIDA,INTRAMURAL RES PROGRAM,BEHAV PHARMACOL & GENET SECT,PRECLIN PHARMACOL LAB,BALTIMORE,MD. JOHNS HOPKINS UNIV,SCH MED,BEHAV BIOL RES CTR,BALTIMORE,MD. FU NIDA NIH HHS [DA-04133] NR 41 TC 30 Z9 30 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1995 VL 272 IS 1 BP 100 EP 111 PG 12 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QB189 UT WOS:A1995QB18900014 PM 7815322 ER PT J AU GU, ZF PRADHAN, TK COY, DH JENSEN, RT AF GU, ZF PRADHAN, TK COY, DH JENSEN, RT TI INTERACTION OF GALANIN FRAGMENTS WITH GALANIN RECEPTORS ON ISOLATED SMOOTH-MUSCLE CELLS FROM GUINEA-PIG STOMACH - IDENTIFICATION OF A NOVEL GALANIN RECEPTOR SUBTYPE SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID VASOACTIVE-INTESTINAL-PEPTIDE; GENE-RELATED PEPTIDE; INTERNAL ANAL-SPHINCTER; PANCREATIC ACINAR-CELLS; STRUCTURAL REQUIREMENTS; CIRCULAR MUSCLE; AUTORADIOGRAPHIC LOCALIZATION; BINDING-SITES; RAT; ANTAGONISTS AB From structure-function studies it has been proposed that two subtypes of receptors may mediate galanin's actions in the gastrointestinal tract and other tissues and their effects can be either direct or neurally mediated. We have recently demonstrated that isolated gastric smooth muscle cells possess high-affinity galanin receptors, activation of which increases AMP and causes relaxation. Because this cell system contains no neural elements, contains a single class of galanin receptors and allows binding to be correlated with function, it is a good system to investigate peptide requirements for cell activation. Porcine galanin (p-Gal) and rat galanin (r-Gal) were equipotent to the NH2-terminal fragments r,p-Gal(1-10), r,p-Gal(1-15), r,p-Gal(1-20), p-Gal(2-29) and p-Gal(3-29) at inhibiting binding of I-125-galanin to gastric smooth muscle cells from guinea pig (K-d 5-8 nM). The midmolecule fragment p-Gal(9-25) and the long COOH-terminal fragment r-Gal(9-29) were equipotent and 25-fold less potent than p-Gal. Acetylation of r-Gal(9-29) increased potency to that of p-Gal. The short COOH-terminal fragment, p-Gal(21-29) and r-Gal(21-29), had very low affinity (K-d3 mu M). Each peptide alone (1 mu M) caused no effect on cell length, but inhibited carbachol-induced contraction. The inhibition was 81% to 92% for r-Gal or p-Gal, r,p-Gal(1-10), r,p-Gal(1-15), r,p-Gal(1-20), p-Gal(2-29), p-Gal(3-29) and Ac-r-Gal(9-29); 47% for p-Gal(9-25) and r-Gal(9-29); and 16% to 17% for p-Gal(21-29). Dose-response curves showed r-Gal and p-Gal, r,p-Gal(1-20) and p-Gal(3-29) had similar EC(50) values (3-8 nM). p-Gal(21-29) and r-Gal(21-29) were much less potent causing relaxation only at concentrations > 10 mu M. There was a close correlation between the relaxant ability and binding affinity for each fragment. Each fragment at 1 mu M increased cAMP similar to its relaxant activity. These results demonstrate that in guinea pig gastric smooth muscle cells the structure-function relationship for galanin is different from that described in other cell systems. This finding, coupled with a recent study demonstrating that the chimeric galanin receptor antagonists M-15 and M-35 have high affinity and function as agonists in these cells, demonstrates that this cell possesses a galanin receptor with unique properties. In contrast to other cell systems, this galanin receptor has high affinity for both NH2-terminal analogs and p-Gal(3-29), high affinity for reported chimeric antagonists M-15 and M-35, but in both cases each of these chimeric antagonists function as a full agonist not as an antagonist. These results suggest there are at least three subtypes of galanin receptors. C1 NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. TULANE UNIV,MED CTR,PEPTIDE RES LABS,NEW ORLEANS,LA. FU NIDDK NIH HHS [DK-18370] NR 56 TC 49 Z9 50 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1995 VL 272 IS 1 BP 371 EP 378 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QB189 UT WOS:A1995QB18900045 PM 7529309 ER PT J AU EVANS, RL TURNER, RJ AF EVANS, RL TURNER, RJ TI UP-REGULATION OF THE NA+-K+-2CL(-) COTRANSPORTER BY MUSCARINIC STIMULATION IN PAROTID ACINAR-CELLS ISOLATED FROM THE RAT SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Meeting Abstract C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. NR 1 TC 1 Z9 1 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD JAN PY 1995 VL 482P BP P7 EP P8 PG 2 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA QE655 UT WOS:A1995QE65500012 ER PT J AU HE, X KUIJPERS, GA POLLARD, HB BAUM, BJ AF HE, X KUIJPERS, GA POLLARD, HB BAUM, BJ TI CELL-SURFACE POLARITY MAINTAINED IN CULTURED MONOLAYERS OF IMMORTALIZED RAT SUBMANDIBULAR EPITHELIAL-CELLS SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Meeting Abstract C1 NIDR,CIPCB,BETHESDA,MD 20892. NIDDK,LCBG,BETHESDA,MD 20892. NR 2 TC 0 Z9 0 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD JAN PY 1995 VL 482P BP P35 EP P36 PG 2 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA QE655 UT WOS:A1995QE65500060 ER PT J AU SHIPSTON, MJ ARMSTRONG, DL AF SHIPSTON, MJ ARMSTRONG, DL TI ACTIVATION OF PROTEIN-KINASE-C INHIBITS LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS IN CLONAL (GH(4)C(1)) RAT ANTERIOR-PITUITARY-CELLS SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Meeting Abstract C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. UNIV EDINBURGH,DEPT PHARMACOL,EDINBURGH EH8 7NA,MIDLOTHIAN,SCOTLAND. RI Shipston, Mike/C-7309-2013 OI Shipston, Mike/0000-0001-7544-582X NR 1 TC 0 Z9 0 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD JAN PY 1995 VL 482P BP P10 EP P11 PG 2 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA QE655 UT WOS:A1995QE65500017 ER PT J AU MCCARTHY, GR MORGAN, VL AF MCCARTHY, GR MORGAN, VL TI USEFUL MODIFICATIONS OF AN IMPLANT FIXTURE MOUNT SO JOURNAL OF PROSTHETIC DENTISTRY LA English DT Note RP MCCARTHY, GR (reprint author), NIDR,BLDG 10,RM 1N113,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0022-3913 J9 J PROSTHET DENT JI J. Prosthet. Dent. PD JAN PY 1995 VL 73 IS 1 BP 104 EP 104 DI 10.1016/S0022-3913(05)80279-3 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA QC311 UT WOS:A1995QC31100016 PM 7699589 ER PT J AU MIRSKY, AF YARDLEY, SL JONES, BP WALSH, D KENDLER, KS AF MIRSKY, AF YARDLEY, SL JONES, BP WALSH, D KENDLER, KS TI ANALYSIS OF THE ATTENTION-DEFICIT IN SCHIZOPHRENIA - A STUDY OF PATIENTS AND THEIR RELATIVES IN IRELAND SO JOURNAL OF PSYCHIATRIC RESEARCH LA English DT Article ID SUSTAINED ATTENTION; SIGNAL-DETECTION; RISK; DIAGNOSIS; CHILDREN AB County Roscommon, a rural area in the western part of Ireland, was the site of a family study of schizophrenia. As part of this study, we have assessed several elements of attention, identified by principal components analysis in previous investigations, in a group of subjects with schizophrenia, first-degree relatives of subjects with schizophrenia and age- and education-matched controls. The schizophrenic subjects performed significantly more poorly than the controls; the performance of the relatives fell somewhere between the other two groups. Those relatives with a DSM-III-R diagnosis (most frequently, alcohol abuse or an affective disorder) tended to perform more poorly on some of the attention elements than relatives without a diagnosis; in contrast, control subjects with diagnoses were not distinguishable from other controls. The attention elements appeared to differ in their capacity to differentiate the groups and each seemed to have a distinctive profile. The effects of alcohol abuse were also considered. The results obtained with this cohort may provide clues concerning the pathophysiological basis of schizophrenia and the heterogeneity of its expression. RP MIRSKY, AF (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. FU NIMH NIH HHS [MH-41953] NR 65 TC 56 Z9 58 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0022-3956 J9 J PSYCHIAT RES JI J. Psychiatr. Res. PD JAN-FEB PY 1995 VL 29 IS 1 BP 23 EP 42 DI 10.1016/0022-3956(94)00041-O PG 20 WC Psychiatry SC Psychiatry GA QX079 UT WOS:A1995QX07900004 PM 7629753 ER PT J AU Brown, LJ Selwitz, RH AF Brown, LJ Selwitz, RH TI The impact of recent changes in the epidemiology of dental caries on guidelines for the use of dental sealants SO JOURNAL OF PUBLIC HEALTH DENTISTRY LA English DT Article DE epidemiology of dental caries; pit and fissure sealants; guidelines for sealant placement; sealant programs ID RISK ASSESSMENT; FISSURE SEALANTS; UNITED-STATES; NATIONAL SURVEYS; SCHOOL-CHILDREN; PREVALENCE; FLUORIDE; SCHOOLCHILDREN; PREDICTION; FLUOROSIS AB This paper reviews recent changes in the epidemiology of dental caries and assesses their potential impact on the diagnosis and management of the disease and the planning and operation of sealant programs. These changes, such as the decline in caries and slowing of the rate of progression of the disease, have important implications for diagnosing and treating incipient lesions, predicting caries risk, and conducting effective disease preventive programs. Traditional paradigms for restoring carious lesions are being replaced by newer strategies that emphasize disease prevention and conservation of tooth structure. The search continues for the identification of practical models for predicting caries risk at the individual level. This paper describes a method useful for targeting resources in sealant placement programs by enabling one to determine the relative effectiveness of sealing alternative tooth surfaces in the oral cavity. One guide serves as a widely adopted manual for those who use or intend to use dental sealants in caries prevention programs. This paper provides a brief review of that document, ''Preventing Pit and Fissure Caries: A Guide to Sealant Use, ''as well as guidelines for sealant utilization provided by the American Dental Association, and information regarding dental sealant programs under Medicaid. The final portion of the paper provides a synthesis of the epidemiology reviewed and summarizes the implications of findings for sealant programs. RP Brown, LJ (reprint author), NIDR,DIV EPIDEMIOL & ORAL DIS PREVENT,NATCHER BLDG,ROOM 4AS 13E,45 CTR DR,BETHESDA,MD 20892, USA. NR 102 TC 49 Z9 50 U1 2 U2 4 PU AAPHD NATIONAL OFFICE PI RICHMOND PA J PUBLIC HEALTH DENT 10619 JOUSTING LANE, RICHMOND, VA 23235 SN 0022-4006 J9 J PUBLIC HEALTH DENT JI J. Public Health Dent. PY 1995 VL 55 IS 5 SI SI BP 274 EP 291 DI 10.1111/j.1752-7325.1995.tb02382.x PG 18 WC Dentistry, Oral Surgery & Medicine; Public, Environmental & Occupational Health SC Dentistry, Oral Surgery & Medicine; Public, Environmental & Occupational Health GA VB626 UT WOS:A1995VB62600004 PM 8854268 ER PT J AU HOLDER, H BOYD, G HOWARD, J FLAY, B VOAS, R GROSSMAN, M AF HOLDER, H BOYD, G HOWARD, J FLAY, B VOAS, R GROSSMAN, M TI ALCOHOL-PROBLEM PREVENTION RESEARCH POLICY - THE NEED FOR A PHASES RESEARCH MODEL SO JOURNAL OF PUBLIC HEALTH POLICY LA English DT Article ID SERVER INTERVENTION; CANCER PREVENTION; HEALTH; CONSUMPTION; EDUCATION; PROGRAMS; DESIGN; TRIALS AB This paper describes the need and rationale for developing a phases model for guiding alcohol-problem prevention research. A phased approach to prevention research is consistent with such models developed for other health areas including heart disease, cancer, and drug testing. Such a model in alcohol prevention research can provide a means for (I) locating how far research has progressed along a continuum from basic or pre-intervention research to full implementation of preventive action, (2) identifying gaps in research, and (3) determining the level of empirical proof which exists for one or more prevention strategies prior to widespread dissemination. C1 NIAAA,CLIN PREVENT RES,PREVENT RES BRANCH,ROCKVILLE,MD 20892. UNIV ILLINOIS,PREVENT RES CTR,CHICAGO,IL 60607. PACIFIC INST RES & EVALUAT,BETHESDA,MD 20814. CUNY,GRAD SCH,NEW YORK,NY 10036. RP HOLDER, H (reprint author), PREVENT RES CTR,2150 SHATTUCK AVE,SUITE 900,BERKELEY,CA 94704, USA. OI Flay, Brian/0000-0002-5209-2766 NR 42 TC 8 Z9 8 U1 1 U2 1 PU JOURNAL PUBLIC HEALTH POLICY PI S BURLINGTON PA 208 MEADOWOOD DR, S BURLINGTON, VT 05403 SN 0197-5897 J9 J PUBLIC HEALTH POL JI J. Public Health Policy PY 1995 VL 16 IS 3 BP 324 EP 346 DI 10.2307/3342862 PG 23 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TB422 UT WOS:A1995TB42200005 PM 7499514 ER PT J AU HERTZMAN, PA KAUFMAN, LD LOVE, LA MEASE, PJ PHILEN, RM PINCUS, T ROSENBERG, NL SILVER, R VARGA, J CLAUW, DJ AF HERTZMAN, PA KAUFMAN, LD LOVE, LA MEASE, PJ PHILEN, RM PINCUS, T ROSENBERG, NL SILVER, R VARGA, J CLAUW, DJ TI THE EOSINOPHILIA-MYALGIA-SYNDROME - GUIDELINES FOR PATIENT-CARE SO JOURNAL OF RHEUMATOLOGY LA English DT Editorial Material ID TRYPTOPHAN; MANIFESTATIONS; SPECTRUM C1 SUNY STONY BROOK,STONY BROOK,NY 11794. US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204. MINOR & JAMES MED CLIN,SEATTLE,WA. CTR DIS CONTROL & PREVENT,ATLANTA,GA 30341. VANDERBILT UNIV,SCH MED,DEPT RHEUMATOL,NASHVILLE,TN 37212. COLORADO NEUROL INST,ENGLEWOOD,CO. MED UNIV S CAROLINA,DIV RHEUMATOL & IMMUNOL,CHARLESTON,SC 29425. NIMH,ADAMHA,BETHESDA,MD 20892. THOMAS JEFFERSON UNIV,DEPT RHEUMATOL,PHILADELPHIA,PA 19107. GEORGETOWN UNIV,SCH MED,DEPT RHEUMATOL,WASHINGTON,DC. RP HERTZMAN, PA (reprint author), LOS ALAMOS MED CTR,LOS ALAMOS,NM 87544, USA. NR 16 TC 4 Z9 4 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JAN PY 1995 VL 22 IS 1 BP 161 EP 163 PG 3 WC Rheumatology SC Rheumatology GA QA693 UT WOS:A1995QA69300030 PM 7699664 ER PT J AU PARCEL, GS EDMUNDSON, E PERRY, CL FELDMAN, HA OHARATOMPKINS, N NADER, PR JOHNSON, CC STONE, EJ AF PARCEL, GS EDMUNDSON, E PERRY, CL FELDMAN, HA OHARATOMPKINS, N NADER, PR JOHNSON, CC STONE, EJ TI MEASUREMENT OF SELF-EFFICACY FOR DIET-RELATED BEHAVIORS AMONG ELEMENTARY-SCHOOL-CHILDREN SO JOURNAL OF SCHOOL HEALTH LA English DT Article ID PHYSICAL-ACTIVITY; HEALTHFUL DIET; CARDIOVASCULAR HEALTH; PROMOTION AB Health promotion interventions intended to improve dietary behavior frequently incorporate self-efficacy as a construct to enhance behavior change. This paper presents results from a study to establish psychometric properties of a scale to measure children's self-efficacy for selecting healthful food. As part of a series of pilot studies to develop instrumentation for the Child and Adolescent Trial for Cardiovascular Health (CATCH), data were collected on third and fourth grade students (n = 1,127). Data analyses were conducted to estimate internal consistency, test-retest reliability, factorial validity, and criterion related validity. Results revealed acceptable estimates of internal consistency for the dietary self-efficacy scale (coefficient alpha = .84). Self-efficacy was strong associated with the children's usual food choices, accounting for about 34% of variance (Multiple R = .58). Findings support using such an instrument for evaluating intervention programs addressing nutrition behavior and for studies to determine the association of self-efficacy to dietary behavior or related constructs. C1 UNIV TEXAS,DEPT KINESIOL,AUSTIN,TX 78712. UNIV MINNESOTA,SCH PUBL HLTH,DIV EPIDEMIOL,MINNEAPOLIS,MN 55454. NEW ENGLAND RES INST INC,WATERTOWN,MA 01372. UNIV CALIF SAN DIEGO,DEPT PEDIAT CHILD & FAMILY HLTH SERV,LA JOLLA,CA 92093. TULANE UNIV,DEPT PUBL HLTH & TROP MED,NEW ORLEANS,LA 70112. NHLBI,CATCH,PREVENT & DEMONSTRAT BRANCH,DIV EPIDEMIOL & CLIN APPLICAT,BETHESDA,MD 20892. RP PARCEL, GS (reprint author), UNIV TEXAS,HLTH SCI CTR,CTR HLTH PROMOT RES & DEV,HOUSTON,TX 77225, USA. FU NHLBI NIH HHS [U01 HL 39852, U01 HL 39927, U01 HL 39870] NR 26 TC 71 Z9 71 U1 0 U2 7 PU AMER SCHOOL HEALTH ASSOC PI KENT PA PO BOX 708, KENT, OH 44240 SN 0022-4391 J9 J SCHOOL HEALTH JI J. Sch. Health PD JAN PY 1995 VL 65 IS 1 BP 23 EP 27 PG 5 WC Education & Educational Research; Education, Scientific Disciplines; Health Care Sciences & Services; Public, Environmental & Occupational Health SC Education & Educational Research; Health Care Sciences & Services; Public, Environmental & Occupational Health GA QF169 UT WOS:A1995QF16900004 PM 7731197 ER PT J AU QUAN, H YU, KF AF QUAN, H YU, KF TI SEQUENTIAL TESTING WITH INTERVAL CENSORED-DATA SO JOURNAL OF STATISTICAL COMPUTATION AND SIMULATION LA English DT Article DE TESTING POWER; EXPECTED STOPPING TIME; MEAN LIFETIME; EXPONENTIAL DISTRIBUTION ID CLINICAL-TRIALS AB Let X(1),..., X(n) be the lifetimes of n items put on testing at the same time. It is not possible to observe the actual lifetimes. However, it is possible to inspect the items at a finite number of time intervals. At each time of inspection, the number of failures can be recorded. Only these numbers of failures at times of inspections will be available to make decision on the distribution of the lifetimes. Decision can be made at the time of inspection. A ''sequential'' statistical test is developed to test the mean levels of the lifetimes when the probability distribution is assumed to be exponential. Some numerical results will be presented. The power and the expected time for the decision are compared with those for the idealized situation when each and every actual lifetime is recorded. They are also compared with those for the case when one and only one inspection is allowed to make the decision. C1 NICHHD,DIV EPIDEMIOL STAT & PREVENT RES,BIOMETRY & MATH STAT BRANCH,BETHESDA,MD 20892. RP QUAN, H (reprint author), MERCK SHARP & DOHME LTD,RES LABS,CLIN BIOSTAT & RES DATA SYST,WBD-400,POB 2000,RAHWAY,NJ 07065, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU GORDON BREACH SCI PUBL LTD PI READING PA C/O STBS LTD PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0094-9655 J9 J STAT COMPUT SIM JI J. Stat. Comput. Simul. PY 1995 VL 51 IS 2-4 BP 239 EP 247 DI 10.1080/00949659508811635 PG 9 WC Computer Science, Interdisciplinary Applications; Statistics & Probability SC Computer Science; Mathematics GA QR593 UT WOS:A1995QR59300010 ER PT J AU TEIEN, DE JONES, M SHIOTA, T YAMADA, I SAHN, DJ AF TEIEN, DE JONES, M SHIOTA, T YAMADA, I SAHN, DJ TI DOPPLER EVALUATION OF SEVERITY OF MITRAL REGURGITATION - RELATION TO PULMONARY VENOUS-BLOOD FLOW PATTERNS IN AN ANIMAL STUDY SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID LEFT ATRIAL PRESSURE; TRANSESOPHAGEAL ECHOCARDIOGRAPHY AB Objectives. This study examined the influence of regurgitant volume on pulmonary venous blood dow patterns in an animal model with quantifiable mitral regurgitation. Background. Systolic pulmonary venous blood how is influenced by atrial filling and compliance and ventricular output and by the presence of mitral regurgitation. The quantitative severity of the regurgitant volume itself is difficult to judge in clinical examinations. Methods. Six sheep,vith chronic mitral regurgitation produced by previous operation to create chordal damage were examined. At reoperation the heart was exposed and epicardial echocardiography performed. Pulmonary venous blood flow waveforms were recorded by pulsed Doppler under color flow Doppler guidance using a Vingmed 750 scanner. The pulmonary venous systolic inflow to the left atrium was expressed as a fraction of the total inflow velocity time integral. Flows across the aortic and mitral valves were recorded by electromagnetic flowmeters balanced against each other. Pressures in the left ventricle and left atrium were measured directly with high fidelity manometer-tipped catheters, Preload and afterload were systematically manipulated, resulting in 24 stable hemodynamic states. Results. Simple logarithmic correlation between the regurgitant volume and size of a positive or negative pulmonary venous inflow velocity time integral during systole was good (r = -0.841), By stepwise linear regression analysis with pulmonary venous negative systolic velocity time integral as a dependent variable compared with the regurgitant volume, fractional shortening, left atrial nu wave size, systemic vascular resistance and left ventricular systolic pressure, only contributions from nu wave size and regurgitant volume (r = 0.80) reached statistical significance in determining pulmonary venous negative systolic flow. Conclusions. Evaluation of systolic pulmonary venous blood how velocity time integral can give valuable information helpful for estimating the regurgitant volume secondary to mitral regurgitation. C1 OREGON HLTH SCI UNIV,DOERNBECHER MEM HOSP CHILDREN,CLIN CARE CTR CONGENITAL HEART DIS,PORTLAND,OR 97201. NHLBI,BETHESDA,MD 20892. FU NHLBI NIH HHS [HL 43287] NR 17 TC 18 Z9 18 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD JAN PY 1995 VL 25 IS 1 BP 264 EP 268 DI 10.1016/0735-1097(94)00338-Q PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QL249 UT WOS:A1995QL24900046 PM 7798514 ER PT J AU HOROWITZ, AM NOURJAH, P GIFT, HC AF HOROWITZ, AM NOURJAH, P GIFT, HC TI UNITED-STATES ADULT KNOWLEDGE OF RISK-FACTORS AND SIGNS OF ORAL CANCERS - 1990 SO JOURNAL OF THE AMERICAN DENTAL ASSOCIATION LA English DT Article ID DRINKING; SMOKING AB A sample of U.S. adults were asked questions to assess their knowledge of oral cancer as part of the 1990 Health Promotion and Disease Prevention Supplement of the National Health Interview Survey. Tobacco use was the only risk factor most adults identified correctly. Only 25 percent of those surveyed could identify one sign of oral cancer. Overall, there is extensive misinformation and a general lack of knowledge. C1 WESTAT CORP,BETHESDA,MD. RP HOROWITZ, AM (reprint author), NIDR,DIS PREVENT & HLTH PROMOT BRANCH,EPIDEMIOL & ORAL DIS PROGRAM,BETHESDA,MD 20892, USA. NR 19 TC 66 Z9 66 U1 0 U2 2 PU AMER DENTAL ASSN PI CHICAGO PA 211 E CHICAGO AVE, CHICAGO, IL 60611 SN 0002-8177 J9 J AM DENT ASSOC JI J. Am. Dent. Assoc. PD JAN PY 1995 VL 126 IS 1 BP 39 EP 45 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA QB570 UT WOS:A1995QB57000008 PM 7822644 ER PT J AU BAHRO, M SILBER, E SUNDERLAND, T AF BAHRO, M SILBER, E SUNDERLAND, T TI HOW DO PATIENTS WITH ALZHEIMERS-DISEASE COPE WITH THEIR ILLNESS - A CLINICAL-EXPERIENCE REPORT SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID DEMENTIA C1 WASHINGTON PSYCHOANALYT INST,WASHINGTON,DC. RP BAHRO, M (reprint author), NIMH,CTR CLIN,CLIN SCI LAB,GERIATR PSYCHIAT SECT,BLDG 10,ROOM 3D41,BETHESDA,MD 20892, USA. NR 18 TC 62 Z9 61 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JAN PY 1995 VL 43 IS 1 BP 41 EP 46 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QA167 UT WOS:A1995QA16700009 PM 7806738 ER PT J AU HODES, RJ AF HODES, RJ TI OSTEOPOROSIS - EMERGING RESEARCH STRATEGIES AIM AT BONE BIOLOGY, RISK-FACTORS, INTERVENTIONS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID FRACTURES RP HODES, RJ (reprint author), NIA,OFF DIRECTOR,BLDG 31,ROOM 5C35,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 15 TC 4 Z9 4 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JAN PY 1995 VL 43 IS 1 BP 75 EP 77 PG 3 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QA167 UT WOS:A1995QA16700016 PM 7806746 ER PT J AU SLATER, SL KATZ, IR AF SLATER, SL KATZ, IR TI PREVALENCE OF DEPRESSION IN THE AGED - FORMAL CALCULATIONS VERSUS CLINICAL FACTS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Editorial Material ID MAJOR DEPRESSION; SYMPTOMS; DIAGNOSIS; DISORDERS; ILLNESS; CANCER C1 UNIV PENN,GERIATR PSYCHIAT SECT,PHILADELPHIA,PA. RP SLATER, SL (reprint author), NIA,GERIATR PROGRAM,BETHESDA,MD, USA. NR 17 TC 14 Z9 15 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JAN PY 1995 VL 43 IS 1 BP 78 EP 79 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QA167 UT WOS:A1995QA16700017 PM 7806747 ER PT J AU STRIKER, G AF STRIKER, G TI REPORT ON A WORKSHOP TO DEVELOP MANAGEMENT RECOMMENDATIONS FOR THE PREVENTION OF PROGRESSION IN CHRONIC RENAL-DISEASE SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Editorial Material RP STRIKER, G (reprint author), NIDDK,KUH,BETHESDA,MD, USA. NR 4 TC 25 Z9 25 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD JAN PY 1995 VL 5 IS 7 BP 1537 EP 1540 PG 4 WC Urology & Nephrology SC Urology & Nephrology GA QC569 UT WOS:A1995QC56900018 PM 7703393 ER PT J AU KORN, EL GRAUBARD, BI AF KORN, EL GRAUBARD, BI TI ANALYSIS OF LARGE HEALTH SURVEYS - ACCOUNTING FOR THE SAMPLING DESIGN SO JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES A-STATISTICS IN SOCIETY LA English DT Article DE CAUSALITY; CLUSTERING; DESIGN-BASED INFERENCE; DESIGN EFFECTS; MODEL-BASED INFERENCE; SAMPLE WEIGHTS ID NUTRITION-EXAMINATION-SURVEY; PROPORTIONAL HAZARDS MODELS; COMPLEX SURVEY DATA; BODY IRON STORES; STRATIFIED SAMPLES; HISPANIC-HEALTH; SURVEY WEIGHTS; INFERENCE; REGRESSION; STATISTICS AB Large scale health surveys offer an opportunity to study associations between risk factors and outcomes in a population-based setting. Their complicated multistage sampling designs with differential probabilities of sampling individuals can make their analysis unstraightforward. Classical 'design-based' methods that yield approximately unbiased estimators of associations and standard errors can be highly inefficient. Model-based methods require assumptions which, if wrong, can lead to biased estimators of associations and standard errors. This paper examines the implications of utilizing the sample clustering and sample weights in the analysis of survey data. The approach is to estimate the inefficiency of using these aspects of the sampling design in a design-based analysis when actually it was unnecessary to do so. If the inefficiency is small, then that aspect of the design is used in a design-based fashion. Otherwise, additional modelling assumptions are incorporated into the analysis. By focusing attention on risk factor-outcome associations in large health surveys, specific recommendations for practitioners are given. The issues are demonstrated with real survey data including two controversial analyses previously published in medical references. RP KORN, EL (reprint author), NCI,BIOMETR RES BRANCH,EPN-739,BETHESDA,MD 20892, USA. NR 63 TC 77 Z9 77 U1 3 U2 12 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD, OXON, ENGLAND OX4 1JF SN 0035-9238 J9 J ROY STAT SOC A STA JI J. R. Stat. Soc. Ser. A-Stat. Soc. PY 1995 VL 158 BP 263 EP 295 DI 10.2307/2983292 PN 2 PG 33 WC Social Sciences, Mathematical Methods; Statistics & Probability SC Mathematical Methods In Social Sciences; Mathematics GA QT893 UT WOS:A1995QT89300003 ER PT J AU POSTON, CD JAFFE, GS LUBENSKY, IA SOLOMON, D ZBAR, B LINEHAN, WM WALTHER, MM AF POSTON, CD JAFFE, GS LUBENSKY, IA SOLOMON, D ZBAR, B LINEHAN, WM WALTHER, MM TI CHARACTERIZATION OF THE RENAL PATHOLOGY OF A FAMILIAL FORM OF RENAL-CELL CARCINOMA-ASSOCIATED WITH VON HIPPEL-LINDAU DISEASE - CLINICAL AND MOLECULAR-GENETIC IMPLICATIONS SO JOURNAL OF UROLOGY LA English DT Article DE HIPPEL-LINDAU DISEASE; CARCINOMA, RENAL CELL; KIDNEY NEOPLASMS; GENETICS, BIOCHEMICAL; HEREDITARY DISEASES ID MANIFESTATIONS; MEMBERS; TUMORS; DNA AB We examined 12 patients with von Hippel-Lindau disease and renal cell carcinoma to characterize the pathological findings after renal surgery (total 161 lesions). Tissue from 15 partial nephrectomies and 1 radical nephrectomy was obtained for examination. Pathological evaluation identified 116 cystic lesions, 25 of which (21%) were malignant, and 45 solid lesions, 41 of which (91%) were renal cell carcinoma. Each kidney had a mean of 7.8 cystic and 3.0 solid lesions. Of 66 malignant lesions 35 (53%) were comprised of cells with only clear cell cytological features and 30 (45.5%) contained predominantly clear cells with scattered granular cells. One malignant lesion (1.5%) contained sarcomatoid renal cell carcinoma with clear and granular cells. Microscopic invasion of the pseudocapsule was common (13 of 25 clinically cystic tumors [52%] and 31 of 41 clinically solid lesions [76%]) but no tumor extended through the pseudocapsule. All lesions without a pseudocapsule were well circumscribed. Our study establishes the clear cell cytological features as the primary finding observed in patients with von Hippel-Lindau disease. Many cystic lesions contained renal cancer, demonstrating the need for removal of all cystic lesions when this can be safely performed. The well circumscribed nature of the tumors lends them to parenchymal sparing procedures. C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NCI,FREDERICK CANC RES & DEV CTR,IMMUNOBIOL LAB,FREDERICK,MD 21701. RP POSTON, CD (reprint author), NCI,SURG BRANCH,UROL ONCOL SECT,BLDG 10,BETHESDA,MD 20892, USA. NR 24 TC 83 Z9 89 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD JAN PY 1995 VL 153 IS 1 BP 22 EP 26 DI 10.1097/00005392-199501000-00009 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA PW167 UT WOS:A1995PW16700008 PM 7966777 ER PT J AU CHEN, Z BERKOWER, I WANG, RYH CHING, WM ALTER, HJ SHIH, JWK AF CHEN, Z BERKOWER, I WANG, RYH CHING, WM ALTER, HJ SHIH, JWK TI GENETIC-CONTROL OF THE MURINE HUMORAL RESPONSE TO DISTINCT EPITOPES OF HEPATITIS-C VIRUS CORE PROTEIN SO JOURNAL OF VIRAL HEPATITIS LA English DT Article DE EPITOPES; GENETIC REGULATION; HEPATITIS C VIRUS; HUMORAL RESPONSE; MURINE ID NON-B-HEPATITIS; NON-A; POSTTRANSFUSION HEPATITIS; TRANSFUSION HEPATITIS; CLINICAL-FEATURES; VIRAL-HEPATITIS; ANTIGEN; ANTIBODY; PEPTIDE; INFECTION AB Recombinant hepatitis C virus (HCV) core protein from aa1-164, designated cp1-10, was used to immunize mice. Antibodies to cp1-10 were produced in all seven strains of congenic mice; none of the strains could be considered low responders relative to the others. The mouse response against individual epitopes of HCV core protein varied from one strain to another: B10.RIII(H-2(r)) recognized all three peptides aa13-30, aa77-90, aa129-145: B10.D2 (H-2(d)), B10(H-2(b)) and C3H.SW (H-2(b)) responded to aa13-30, aa77-90: B10.M (H-2(f)), B10.BR (H-2(k)) and C3H/HeJ (H-2(k)) reacted with aa13-30 only. Competitive inhibition of binding demonstrated that antibody to the peptide was inhibited by cp1-10 protein and the corresponding peptide only. Recombinant HCV core protein is highly immunogenic and can elicit good antibody response in mice. The aa13-30 is a major epitope of HCV core protein in mice. The humoral response to the distinct epitopes was regulated by the H-2 genes. Further analysis indicated that the I-a locus of H-2 genes determined the antibody response to aa13-30 and 77-90. These results suggest that the variation of antibody responses to HCV in humans may partially contribute to different outcomes of HCV infection. C1 NIH, WARREN GRANT MAGNUSON CLIN CTR, DEPT TRANSFUS MED, BETHESDA, MD 20892 USA. US FDA, CTR BIOL EVALUAT & RES, OFF VACCINE RES & REVIEW, IMMUNOREGULAT LAB, BETHESDA, MD USA. ARMED FORCES INST PATHOL, AMER REGISTRY PATHOL, WASHINGTON, DC 20306 USA. NATL NAVAL MED CTR, DEPT INFECT DIS, BETHESDA, MD 20814 USA. NR 35 TC 27 Z9 28 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 1352-0504 J9 J VIRAL HEPATITIS JI J. Viral Hepatitis PY 1995 VL 2 IS 1 BP 9 EP 17 DI 10.1111/j.1365-2893.1995.tb00067.x PG 9 WC Gastroenterology & Hepatology; Infectious Diseases; Virology SC Gastroenterology & Hepatology; Infectious Diseases; Virology GA RK417 UT WOS:A1995RK41700002 PM 7493296 ER PT J AU ALTER, HJ SANCHEZPESCADOR, R URDEA, MS WILBER, JC LAGIER, RJ DIBISCEGLIE, AM SHIH, JW NEUWALD, PD AF ALTER, HJ SANCHEZPESCADOR, R URDEA, MS WILBER, JC LAGIER, RJ DIBISCEGLIE, AM SHIH, JW NEUWALD, PD TI EVALUATION OF BRANCHED DNA SIGNAL AMPLIFICATION FOR THE DETECTION OF HEPATITIS-C VIRUS-RNA SO JOURNAL OF VIRAL HEPATITIS LA English DT Article DE BRANCHED DNA; HCV RNA; HEPATITIS C VIRUS; INTERFERON FOR HCV INFECTION; MICROWELL ASSAYS; NON-A; NON-B HEPATITIS VIRUS; PCR; POSTTRANSFUSION HEPATITIS; QUANTITATION OF RNA ID NON-B-HEPATITIS; NON-A; HCV; RECIPIENTS; GENOME; ALPHA; HIV AB There is an increasing need for a practical assay to measure HCV RNA to assess the viral burden in chronic hepatitis C virus (HCV) infection as viral load relates to transmission and therapeutic response, This study evaluates branched DNA (bDNA) signal amplification, a technique that avoids many of the pitfalls of polymerase chain reaction (PCR). The bDNA assay uses a microtitre well format and a series of capture, target and amplification probes that bind RNA to the well and then successively bind oligonucleotides to the RNA and branched DNA molecules to the oligonucleotides, Enzyme-labelled probes are bound to the arms of the bDNA and light output from a chemiluminescent substrate is directly proportional to the amount of starting HCV RNA. Appropriate standards provide direct quantitation. Whereas PCR amplifies the HCV genome, bDNA amplifies the hybridization signal. In testing a standardized, coded panel, bDNA showed 100% specificity and detected five of six sera proven to transmit hepatitis C to the chimpanzee; PCR detected all six infectious sera, Serial samples were measured in two acute and five chronic cases of transfusion-associated hepatitis and in three commercial seroconversion panels, In acute cases, 10(7)-10(8) molecular equivalents per mi (eq per mi) of HCV RNA were detected prior to peak alanine aminotransferase (ALT) activity and then rapidly declined to non-detectable levels. Similar levels of HCV RNA were observed early in the course of two patients who progressed to chronic hepatitis; the chronic course was characterized by diminished, fluctuating and sometimes non-detectable levels of HCV RNA, In two chronic cases, HCV RNA was not detected, or only transiently detected by bDNA, but was present when assayed by PCR, In one chronic case, the periodicity of HCV RNA levels closely paralleled the fluctuations of ALT suggesting a relationship between viral replication and subsequent hepatocellular injury, In testing 50 blood donors whose anti-HCV reactivity was confirmed by a recombinant immunoblot assay (RIBA), HCV RNA was detected by bDNA in 41 (81%), while PCR was positive in 45 (90%); the overall concordance between bDNA and PCR in 100 anti-HCV enzyme immunoassays (EIA) reactive donor samples was 96%. Lastly, bDNA showed the loss of HCV RNA in six out of six evaluable patients who had complete biochemical responses to interferon; five out of six non-responders also showed appreciable declines in HCV RNA level, but in only two did HCV RNA drop below the detection limit; these two cases remained PCR positive, Seventeen placebo-treated patients did not lose HCV RNA by either bDNA or PCR, Hence the bDNA assay is a practical means to measure HCV RNA in a variety of clinical settings, Although it is not as sensitive as PCR, it has greater specificity, is directly quantitative, and can be used in any routine laboratory that can perform microwell EIAs. This simplified quantitation may be of particular benefit in evaluating the probability of HCV transmission and the response to anti-viral therapy. C1 CHIRON CORP,NUCLEIC ACIDS SYST,EMERYVILLE,CA 94608. NIDDK,DIGEST DIS BRANCH,LIVER DIS SECT,BETHESDA,MD. RP ALTER, HJ (reprint author), NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT TRANSFUS MED,BLDG 10,RM 1C-711,BETHESDA,MD 20892, USA. NR 23 TC 86 Z9 87 U1 1 U2 4 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 1352-0504 J9 J VIRAL HEPATITIS JI J. Viral Hepatitis PY 1995 VL 2 IS 3 BP 121 EP 132 DI 10.1111/j.1365-2893.1995.tb00017.x PG 12 WC Gastroenterology & Hepatology; Infectious Diseases; Virology SC Gastroenterology & Hepatology; Infectious Diseases; Virology GA RN740 UT WOS:A1995RN74000002 PM 7493306 ER PT J AU FANG, H PINCUS, SH AF FANG, H PINCUS, SH TI UNIQUE INSERTION-SEQUENCE AND PATTERN OF CD4 EXPRESSION IN VARIANTS SELECTED WITH IMMUNOTOXINS FROM HUMAN-IMMUNODEFICIENCY-VIRUS TYPE 1-INFECTED T-CELLS SO JOURNAL OF VIROLOGY LA English DT Article ID EXOTOXIN HYBRID PROTEIN; RICIN-A-CHAIN; INFECTED-CELLS; HIV; RETROVIRUS; INFECTIVITY; REPLICATION; ANTIBODIES; EFFICACY; INVITRO AB To study the variability of human immunodeficiency virus type 1 (HIV-1), we used immunotoxins to select for variants within a population of H9 cells persistently infected with a molecular clone of HIV-1 designated NL4-3. Chimeric immunotoxin CD4-PE40 (a chimeric fusion protein consisting of the amino-terminal two domains of CD4 and the carboxy-terminal domains of Pseudomonas exotoxin A) was used to select for cells lacking cell surface expression of HIV Env (envelope proteins gp160, gp120, and gp41). The cells described here (A1, A7, C9, and E9) fail to express HIV proteins because they have markedly diminished transcription of the integrated provirus (A1, A7, and E9) or no HIV provirus (C9). Analysis demonstrated that two different cloned variants, Al and E9, contain the complementary sequence of tRNA(3)(Lys) (45 bp) inserted 3' to the primer-binding site, followed by a 169-bp deletion through the start of the gag gene. No HIV mRNA was detected by Northern (RNA) blotting, but PCR demonstrated the presence of the viral message. These variants were found very infrequently in the unselected H9/NL4-3 cell population, and they contained proviruses distinct from that found in the dominant population. In addition, all of these variants had similar patterns of CD4 surface expression that allowed them to escape reinfection within the tissue culture. The data are discussed with regard to mechanisms and errors of HIV reverse transcription, as well as the evolution of mutants within a population of persistently infected cells. C1 NIAID,ROCKY MT LABS,MICROBIAL STRUCT & FUNCT LAB,HAMILTON,MT 59840. NR 27 TC 7 Z9 7 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1995 VL 69 IS 1 BP 75 EP 81 PG 7 WC Virology SC Virology GA PW815 UT WOS:A1995PW81500009 PM 7983770 ER PT J AU LISZIEWICZ, J SUN, D TRAPNELL, B THOMSON, M CHANG, HK ENSOLI, B PENG, B AF LISZIEWICZ, J SUN, D TRAPNELL, B THOMSON, M CHANG, HK ENSOLI, B PENG, B TI AN AUTOREGULATED DUAL-FUNCTION ANTITAT GENE FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GENE-THERAPY SO JOURNAL OF VIROLOGY LA English DT Article ID TRANS-ACTIVATOR GENE; TAT PROTEIN; RETROVIRAL VECTOR; NUCLEAR-PROTEIN; CELL-GROWTH; EXPRESSION; HIV; RNA; REPLICATION; AIDS AB One approach to gene therapy for AIDS is to block the replication of human immunodeficiency virus type 1 (HIV-1) by inhibiting the fat gene, whose product activates the expression of all HIV-1 genes. To accomplish this, we constructed an antitat gene expressing an RNA with dual (polymeric TAR and antisense-tat) function in an attempt to both sequester Tat protein and block its translation from mRNA. A minigene consisting of the antitat gene driven by the HIV-1 long terminal repeat was inserted into a double-copy retrovirus vector, such that antitat expression would be upregulated only in HIV-1-infected cells. After transduction of a T-lymphocytic cell line (Molt-3) the antitat gene inhibited HIV-1 replication. This inhibition was inversely correlated with the virus' infectious dose. Virus replication was also inhibited for 5 months in two different T-cell lines after they had been infected at a high multiplicity of infection, suggesting that the antitat gene may be effective over long periods. Importantly, antitat blocked the replication and the cytopathic effect of HIV-1 in human peripheral blood mononuclear cells and led to as much as 4,000-fold inhibition of the replication of an HIV-1 field isolate as well as HIV-1 prototypes maintained in culture. These results suggest that antitat gene therapy has potential use for blocking HIV-1 replication in infected individuals. C1 GENET THERAPY INC,GAITHERSBURG,MD 20878. RP LISZIEWICZ, J (reprint author), NCI,TUMOR CELL BIOL LAB,BLDG 37,RM 6A09,37 CONVENT DR MSC 4255,BETHESDA,MD 20892, USA. RI Ensoli, Barbara/J-9169-2016 OI Ensoli, Barbara/0000-0002-0545-8737 NR 37 TC 26 Z9 27 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1995 VL 69 IS 1 BP 206 EP 212 PG 7 WC Virology SC Virology GA PW815 UT WOS:A1995PW81500024 PM 7983711 ER PT J AU VANDEPOL, SB HOWLEY, PM AF VANDEPOL, SB HOWLEY, PM TI NEGATIVE REGULATION OF THE BOVINE PAPILLOMAVIRUS E5, E6, AND E7 ONCOGENES BY THE VIRAL E1 AND E2 GENES SO JOURNAL OF VIROLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; OPEN READING FRAMES; DNA-REPLICATION; TRANSCRIPTIONAL REPRESSOR; 19-KILODALTON PROTEIN; TRANSFORMING ACTIVITY; MUTATIONAL ANALYSIS; TERMINAL DOMAIN; C127 CELLS; TYPE-1 AB Papillomaviruses induce benign squamous epithelial lesions that infrequently are associated with uncontrolled growth or malignant conversion. The virus-encoded oncogenes are clearly under negative regulation since papillomaviruses can latently infect cells and since different levels of viral oncogene expression are seen within the layers of differentiating infected epitheliomas. We used bovine papillomavirus type 1 (BPV-1) to investigate the mechanisms involved in the negative regulation of transformation. We found that the following two distinct and interacting mechanisms negatively regulate BPV-1 transformation effected by virally encoded trans-acting factors: (i) E2 repressors suppress transformation by the E6 and E7 oncogenes, and (ii) El and the E2 transactivator suppress transformation by the E6, E7, and E5 oncogenes. These systems interact in that the E2 repressors function to relieve the transformation suppression effected by the El and E2 transactivator genes. A BPV-1 mutant that lacked E2 repressors and E1 had greatly augmented transformation capacity. Analysis of this mutant revealed that the enhanced transformation was due to expression of the E6 and E7 genes in the absence of E5, revealing a previously unappreciated potency and synergy for the BPV-1 E6 and E7 oncogenes. C1 NCI,TUMOR VIRUS BIOL LAB,BETHESDA,MD 20892. NR 61 TC 21 Z9 22 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1995 VL 69 IS 1 BP 395 EP 402 PG 8 WC Virology SC Virology GA PW815 UT WOS:A1995PW81500046 PM 7983735 ER PT J AU WILSON, CA EIDEN, MV ANDERSON, WB LEHEL, C OLAH, Z AF WILSON, CA EIDEN, MV ANDERSON, WB LEHEL, C OLAH, Z TI THE DUAL-FUNCTION HAMSTER RECEPTOR FOR AMPHOTROPIC MURINE LEUKEMIA-VIRUS (MULV), 10A1 MULV, AND GIBBON APE LEUKEMIA-VIRUS IS A PHOSPHATE SYMPORTER SO JOURNAL OF VIROLOGY LA English DT Note ID INTERFERENCE; CELLS; INFECTION AB Previously, we showed that the amphotropic receptor homolog in hamster cells functions as a receptor not only for amphotropic murine leukemia viruses and 10A1 murine leukemia virus but also for gibbon ape leukemia virus (C. A. Wilson, K. B. Farrell, and M. V. Eiden, J. Virol. 68:7697-7703, 1994). Here, we demonstrate that this receptor functions as a sodium-dependent Pi transporter and that Na-P, uptake can be specifically blocked following infection with either amphotropic murine leukemia virus, 10A1 murine leukemia virus, or gibbon ape leukemia virus. C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. US FDA,CTR BIOL EVALUAT & RES,DIV CELLULAR & GENE THERAPIES,MOLEC TUMOR BIOL LAB,ROCKVILLE,MD 20892. NCI,CELLULAR ONCOL LAB,BETHESDA,MD 20892. NR 19 TC 45 Z9 45 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1995 VL 69 IS 1 BP 534 EP 537 PG 4 WC Virology SC Virology GA PW815 UT WOS:A1995PW81500064 PM 7983751 ER PT J AU CHANG, YN KENAN, DJ KEENE, JD GATIGNOL, A JEANG, KT AF CHANG, YN KENAN, DJ KEENE, JD GATIGNOL, A JEANG, KT TI DIRECT INTERACTIONS BETWEEN AUTOANTIGEN-LA AND HUMAN-IMMUNODEFICIENCY-VIRUS LEADER RNA (VOL 68, PG 7014, 1994) SO JOURNAL OF VIROLOGY LA English DT Correction, Addition C1 DUKE UNIV,MED CTR,DEPT MICROBIOL,DURHAM,NC 27710. RP CHANG, YN (reprint author), NIAID,MOLEC MICROBIOL LAB,MOLEC VIROL SECT,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 1 TC 9 Z9 9 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1995 VL 69 IS 1 BP 618 EP 619 PG 2 WC Virology SC Virology GA PW815 UT WOS:A1995PW81500081 PM 7983768 ER PT J AU JAZWINSKI, SM HOWARD, BH NAYAK, RK AF JAZWINSKI, SM HOWARD, BH NAYAK, RK TI CELL-CYCLE PROGRESSION, AGING, AND CELL-DEATH SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Editorial Material ID PROTEIN C1 NICHHD,BETHESDA,MD. NIH,DIV RES GRANTS,BETHESDA,MD. RP JAZWINSKI, SM (reprint author), LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,1901 PERDIDO ST,NEW ORLEANS,LA 70112, USA. NR 11 TC 5 Z9 5 U1 0 U2 0 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JAN PY 1995 VL 50 IS 1 BP B1 EP B8 PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QK431 UT WOS:A1995QK43100002 PM 7814773 ER PT J AU SIMONSICK, EM GURALNIK, JM HENNEKENS, CH WALLACE, RB OSTFELD, AM AF SIMONSICK, EM GURALNIK, JM HENNEKENS, CH WALLACE, RB OSTFELD, AM TI INTERMITTENT CLAUDICATION AND SUBSEQUENT CARDIOVASCULAR-DISEASE IN THE ELDERLY SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID PERIPHERAL ARTERIAL-DISEASE; RISK-FACTORS; CLINICAL-EVALUATION; DEFINED POPULATION; PREVALENCE; MORTALITY; FRAMINGHAM; PREDICTOR AB Background. This study reports the prevalence of intermittent claudication (IC) in ambulatory community-resident adults age 65 years or older, compares cardiovascular risk factors and comorbidity of persons with and without IC, and examines the independent association of IC in predicting ail cause and cardiovascular mortality, myocardial infarction, stroke, and disability. Methods. Data are from a pooled sample of 8996 older adults from the East Boston, New Haven, and Iowa sites of the Established Populations for Epidemiologic Studies of the Elderly, conducted between 1982 and 1988. Results. 2.4% and 1.5% of men and women, respectively, reported IC. Persons with IC had significantly higher rates of diabetes and cardiovascular comorbidity than persons without IC, and they were more likely to smoke: Claudication predicted higher rates of mortality, myocardial infarction, stroke, and disability independent of associated cardiovascular conditions and risk factors. Among persons with a history of angina, myocardial infarction, and/or stroke, those who reported IC had a twofold greater risk of cardiovascular mortality. Conclusion. The study demonstrated that IC is an important predictor of mortality and cardiovascular morbidity in ambulatory older adults independent of associated coronary ischemia and cardiovascular disease risk factors. Results suggest that inclusion of a measure of IC improves the prediction of cardiovascular morbidity and mortality in older adults. C1 HARVARD UNIV,SCH MED,DEPT PREVENT MED & CLIN EPIDEMIOL,CHANNING LAB,BOSTON,MA. BRIGHAM & WOMENS HOSP,BOSTON,MA. UNIV IOWA,COLL MED,DEPT PREVENT MED & ENVIRONM HLTH,IOWA CITY,IA. YALE UNIV,SCH PUBL HLTH,NEW HAVEN,CT. RP SIMONSICK, EM (reprint author), NIA,EPIDEMIOL DEMOG & BIOMETRY PROGRAM,7201 WISCONSIN AVE,SUITE 3C-309,BETHESDA,MD 20892, USA. NR 35 TC 22 Z9 22 U1 3 U2 3 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JAN PY 1995 VL 50 IS 1 BP M17 EP M22 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QK431 UT WOS:A1995QK43100014 PM 7814784 ER PT J AU WU, AJ BAUM, BJ SHIP, JA AF WU, AJ BAUM, BJ SHIP, JA TI EXTENDED STIMULATED PAROTID AND SUBMANDIBULAR SECRETION IN A HEALTHY-YOUNG AND OLD POPULATION SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID SALIVARY-GLAND FUNCTION; CHOLINERGIC RECEPTORS; FLOW-RATES; AGE; XEROSTOMIA; PEOPLE AB Background. previous morphological studies have shown that both the human parotid and submandibular glands display age-related reductions in the proportion of fluid secreting acinar cells. In contrast, short-term functional studies of Fluid secretion do not show such a consistent disparity among different-aged persons. This study compared the ability of a population of healthy young and old individuals to secrete saliva From the parotid and submandibular glands for an extended period of time under conditions of intense gustatory stimulation. Methods. Saliva was collected over 30 minutes from 30 healthy, unmedicated individuals using 10% citric acid as a gustatory stimulus. Of the 30 subjects, 15 were young (age range 27-40 years) and 15 were old (age range 60-97 years). Parotid salivary flow rates were determined every minute, and submandibular flow rates were determined at 2, 10, 20, and 30 minutes. A comparison was made between the slopes of the two groups. The slopes were derived from the average salivary Row rate at each time point. Results. There was no difference in the ability of the parotid glands of young and old individuals to secrete saliva. In contrast, the submandibular glands of the elderly individuals did not show the same pattern of secretion when compared to their younger counterparts, who demonstrated increasing Row rates over the test period. Conclusions. The parotid glands of young and old persons are able to maintain high secretory ability under the: stress of intense stimulation for an extended period of time. Conversely, the submandibular gland demonstrates an age-related, statistically significant difference in the pattern of fluid secretion with an intense gustatory stimulus. C1 UNIV MICHIGAN,SCH DENT,ANN ARBOR,MI 48109. RP WU, AJ (reprint author), NIDR,CLIN INVEST & PATIENT CARE BRANCH,BLDG 10,ROOM 1N-113,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 31 TC 23 Z9 24 U1 0 U2 7 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JAN PY 1995 VL 50 IS 1 BP M45 EP M48 PG 4 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QK431 UT WOS:A1995QK43100018 PM 7814788 ER PT B AU Yergey, AL Abrams, SA Vieira, NE Esteban, NV Bronner, F AF Yergey, AL Abrams, SA Vieira, NE Esteban, NV Bronner, F BE Subramanian, KNS Wastney, ME TI Calcium metabolism in pregnancy and lactation: An individual study SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NICHHD,SECT METAB ANAL & MASS SPECTROMETRY,LTPB,NIH,BETHESDA,MD 20892. NR 0 TC 4 Z9 4 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 29 EP 35 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00003 ER PT B AU Patterson, BH Zech, LA Swanson, HA Levander, OA AF Patterson, BH Zech, LA Swanson, HA Levander, OA BE Subramanian, KNS Wastney, ME TI An overview of selenium kinetics in humans SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NCI,BIOMETRY BRANCH,DCPC,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 1 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 241 EP 264 PG 24 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00020 ER PT B AU Yergey, AL AF Yergey, AL BE Subramanian, KNS Wastney, ME TI Overview of calcium metabolic dynamics SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NICHHD,SECT METAB ANAL & MASS SPECTROMETRY,LTPB,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 281 EP 282 PG 2 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00022 ER PT B AU Goans, RE Abrams, SA Vieira, NE Yergey, AL AF Goans, RE Abrams, SA Vieira, NE Yergey, AL BE Subramanian, KNS Wastney, ME TI Frequency response of the calcium control system SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NICHHD,THEORET & PHYS BIOL LAB,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 333 EP 340 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00026 ER PT B AU Aldroubi, A Basser, P Vieira, N AF Aldroubi, A Basser, P Vieira, N BE Subramanian, KNS Wastney, ME TI Optimal sampling for the determination of calcium metabolism SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NIH,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 347 EP 354 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00028 ER PT B AU Zech, LA Greif, PC AF Zech, LA Greif, PC BE Subramanian, KNS Wastney, ME TI SAAMEASE: The language of SAAM and CONSAM SO KINETIC MODELS OF TRACE ELEMENT AND MINERAL METABOLISM DURING DEVELOPMENT LA English DT Proceedings Paper CT Mathematical Modeling in Experimental Nutrition IV - Trace Element/Mineral Metabolism During Development Conference CY JUN 07-12, 1992 CL GEORGETOWN UNIV MED CTR, WASHINGTON, DC SP NIH, US EPA, Ross Labs, Georgetown Univ Childrens Med Ctr HO GEORGETOWN UNIV MED CTR C1 NCI,MATH BIOL LAB,DCBD,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 BN 0-8493-4736-X PY 1995 BP 373 EP 387 PG 15 WC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics SC Endocrinology & Metabolism; Nutrition & Dietetics; Obstetrics & Gynecology; Pediatrics GA BF54C UT WOS:A1995BF54C00031 ER PT J AU BORKOWSKI, A RAFFELD, M BENNETT, WP JONES, RT HARRIS, CC BERMAN, JJ BORKOWSKI, P FERREIRA, LR KAO, GF TRUMP, BF AF BORKOWSKI, A RAFFELD, M BENNETT, WP JONES, RT HARRIS, CC BERMAN, JJ BORKOWSKI, P FERREIRA, LR KAO, GF TRUMP, BF TI BCL-2 ONCOPROTEIN EXPRESSION AND KI-67 PROLIFERATION INDEX IN KERATOACANTHOMAS AND SQUAMOUS-CELL CARCINOMAS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV MARYLAND,BALTIMORE,MD 21201. VET ADM MED CTR,BALTIMORE,MD 21218. MT SINAI MED CTR,MIAMI BEACH,FL 33140. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A46 EP A46 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900271 ER PT J AU CAMPO, E MUNOZ, J NADAL, A JARES, P FERNANDEZ, PL PALACIN, A EMMERTBUCK, M SLOANE, B CARDESA, A AF CAMPO, E MUNOZ, J NADAL, A JARES, P FERNANDEZ, PL PALACIN, A EMMERTBUCK, M SLOANE, B CARDESA, A TI CATHEPSIN-B MESSENGER-RNA OVEREXPRESSION CORRELATES WITH TUMOR PROGRESSION IN COLORECTAL CARCINOMAS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV BARCELONA,HOSP CLIN,BARCELONA,SPAIN. UNIV LLEIDA,LLEIDA,SPAIN. NCI,BETHESDA,MD 20892. WAYNE STATE UNIV,DETROIT,MI. RI Sloane, Bonnie/A-1050-2009 NR 0 TC 2 Z9 2 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A58 EP A58 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900344 ER PT J AU CHUAQUI, R ZHUANG, Z EMMERTBUCK, MR BRYANT, B NOGALES, F TAVASSOLI, T MERINO, MJ AF CHUAQUI, R ZHUANG, Z EMMERTBUCK, MR BRYANT, B NOGALES, F TAVASSOLI, T MERINO, MJ TI GENETIC-ANALYSIS OF SYNCHRONOUS TUMORS OF OVARY AND APPENDIX TO DETERMINE SITE OF ORIGIN SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. UNIV GRANADA,GRANADA,SPAIN. FAIRFAX HOSP,FAIRFAX,VA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A87 EP A87 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900517 ER PT J AU CLARKE, MR KIM, R CHANG, T STETLERSTEVENSON, WG REYNOLDS, JC AF CLARKE, MR KIM, R CHANG, T STETLERSTEVENSON, WG REYNOLDS, JC TI BARRETT METAPLASIA AND BARRETT ADENOCARCINOMA OF THE ESOPHAGUS - MIB-1, BCL-2, TYPE-IV COLLAGEN, TYPE-IV COLLAGENASE AND CATHEPSIN-D EXPRESSION SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV PITTSBURGH,SCH MED,PITTSBURGH,PA. NCI,BETHESDA,MD 20892. RI Ribeiro Jr, Ulysses/G-5942-2012; Safatle-Ribeiro, Adriana/H-7378-2012 OI Safatle-Ribeiro, Adriana/0000-0001-7686-8859 NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A59 EP A59 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900347 ER PT J AU DEBELENKE, LV ZHUANG, Z EMMERTBUCK, MR LUBENSKY, IA AF DEBELENKE, LV ZHUANG, Z EMMERTBUCK, MR LUBENSKY, IA TI STUDY OF ALLELIC LOSS ON CHROMOSOME 11Q13 IN SPORADIC AND FAMILIAL INSULINOMAS AND GASTRINOMAS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A129 EP A129 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900769 ER PT J AU DOWNING, J SHURTLEFF, S MEYERS, S HIEBERT, S RAIMONDI, S HEAD, D WILLMAN, C WOLMAN, S CARROLL, A BEHM, F COLLINS, F LIU, P AF DOWNING, J SHURTLEFF, S MEYERS, S HIEBERT, S RAIMONDI, S HEAD, D WILLMAN, C WOLMAN, S CARROLL, A BEHM, F COLLINS, F LIU, P TI HETEROGENEITY IN CBF-BETA/MYH11 FUSION MESSAGES ENCODED BY INV(16) AND T(16-16) IN ACUTE MYELOGENOUS LEUKEMIA (AML) SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 ST JUDE CHILDRENS RES HOSP, MEMPHIS, TN 38105 USA. NIH, BETHESDA, MD 20892 USA. SW ONCOL GRP, SAN ANTONIO, TX USA. PEDIAT ONCOL GRP, CHICAGO, IL USA. RI Liu, Paul/A-7976-2012 OI Liu, Paul/0000-0002-6779-025X NR 0 TC 0 Z9 0 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A109 EP A109 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900646 ER PT J AU EMMERTBUCK, MR CHUAQUI, R ZHUANG, ZP NOGALES, F STETLERSTEVENSON, WG MERINO, MJ AF EMMERTBUCK, MR CHUAQUI, R ZHUANG, ZP NOGALES, F STETLERSTEVENSON, WG MERINO, MJ TI MOLECULAR ANALYSIS OF CONCOMITANT UTERINE AND OVARIAN ENDOMETERIOD TUMORS TO ESTABLISH SITE OF ORIGIN SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV GRANADA,GRANADA,SPAIN. NCI,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A89 EP A89 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900527 ER PT J AU EMMERTBUCK, MR ROTH, MJ TERUYAFELDSTEIN, J MEDEIROS, LJ AF EMMERTBUCK, MR ROTH, MJ TERUYAFELDSTEIN, J MEDEIROS, LJ TI DETECTION OF NM-23 MICROSATELLITE INSTABILITY IN MICRODISSECTED HUMAN PROSTATE-CANCER SPECIMENS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. RHODE ISL HOSP,DEPT PATHOL,PROVIDENCE,RI 02902. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A75 EP A75 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900443 ER PT J AU FLEMING, MV GUINEE, DG CHU, WS TRAVIS, WD BENNETT, WP COLBY, TV TAZELAAR, H ABBONDANZO, SL CAPORASO, NE JETT, J PAIROLERO, P TRASTEK, V LIOTTA, LA HARRIS, CC AF FLEMING, MV GUINEE, DG CHU, WS TRAVIS, WD BENNETT, WP COLBY, TV TAZELAAR, H ABBONDANZO, SL CAPORASO, NE JETT, J PAIROLERO, P TRASTEK, V LIOTTA, LA HARRIS, CC TI CORRELATES OF BCL-2 IMMUNOHISTOCHEMICAL STAINING IN A SURGICAL SERIES OF LUNG-CANCER PATIENTS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892. NCI,PATHOL LAB,BETHESDA,MD 20892. ARMED FORCES INST PATHOL,DIV PULM & MEDIASTINAL,WASHINGTON,DC. MAYO CLIN & MAYO FDN,ROCHESTER,MN. NCI,GENET EPIDEMIOL BRANCH,BETHESDA,MD. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A148 EP A148 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900879 ER PT J AU HAYASHI, T FLEMING, MV FISHBACK, N STETLERSTEVENSON, WG KOSS, MN LIOTTA, LA FERRANS, VJ TRAVIS, WD AF HAYASHI, T FLEMING, MV FISHBACK, N STETLERSTEVENSON, WG KOSS, MN LIOTTA, LA FERRANS, VJ TRAVIS, WD TI IMMUNOHISTOCHEMICAL DISTRIBUTION OF METALLOPROTEINASES IN IDIOPATHIC PULMONARY FIBROSIS (IPF) AND DIFFUSE ALVEOLAR DAMAGE (DAD) SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 ARMED FORCES INST PATHOL,WASHINGTON,DC. NHLBI,PATHOL BRANCH,BETHESDA,MD. NCI,PATHOL LAB,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A148 EP A148 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900881 ER PT J AU HIJAZI, Y FETSCH, P AF HIJAZI, Y FETSCH, P TI IMMUNOREACTIVITY FOR CA-19-9 IN MALIGNANT MESOTHELIOMA AND ADENOCARCINOMA SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A40 EP A40 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900234 ER PT J AU HIJAZI, Y SOLOMON, D AF HIJAZI, Y SOLOMON, D TI ADULT T-CELL LEUKEMIA-LYMPHOMA (ATLL) - A CYTOPATHOLOGICAL AND IMMUNOCYTOCHEMICAL STUDY SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A40 EP A40 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900233 ER PT J AU KELETI, J TSOKOS, M RAFFELD, M AF KELETI, J TSOKOS, M RAFFELD, M TI LAZ3 IS DIFFERENTIALLY EXPRESSED IN NONHEMATOLOGICAL TUMOR-CELL LINES SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A139 EP A139 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900828 ER PT J AU KINGMA, DW WEISS, WB JAFFE, ES KUMAR, S RAFFELD, M AF KINGMA, DW WEISS, WB JAFFE, ES KUMAR, S RAFFELD, M TI EBV LMP1 ONCOGENE DELETIONS - CORRELATIONS WITH MALIGNANCY IN EBV-ASSOCIATED LYMPHOPROLIFERATIVE DISORDERS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A114 EP A114 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900675 ER PT J AU KLEINER, DE GAFFEY, MJ SALLIE, R TSOKOS, M NICHOLS, L FOX, C MCKENZIE, R FRIED, MW STRAUS, S HOOFNAGLE, JH AF KLEINER, DE GAFFEY, MJ SALLIE, R TSOKOS, M NICHOLS, L FOX, C MCKENZIE, R FRIED, MW STRAUS, S HOOFNAGLE, JH TI PATHOLOGICAL FEATURES OF FLUOROIODOARABINOFURANOSYL URACIL (FIAU) HEPATOTOXICITY IN PATIENTS WITH CHRONIC HEPATITIS-B (CHB) SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. UNIV VIRGINIA HOSP,CHARLOTTESVILLE,VA. UNIV PITTSBURGH,PITTSBURGH,PA 15260. EMORY UNIV,ATLANTA,GA 30322. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A131 EP A131 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900780 ER PT J AU LITZKY, LA MOONEY, AM AMIN, K SMYTHE, WR MORRIS, JM PASS, HI RODECK, U RAUSCHER, FJ KAISER, LR ALBELDA, SM AF LITZKY, LA MOONEY, AM AMIN, K SMYTHE, WR MORRIS, JM PASS, HI RODECK, U RAUSCHER, FJ KAISER, LR ALBELDA, SM TI THE WILMS-TUMOR GENE-PRODUCT IS A USEFUL MARKER FOR THE DIAGNOSIS OF MALIGNANT MESOTHELIOMA SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV PENN,MED CTR,PHILADELPHIA,PA. WISTAR INST ANAT & BIOL,PHILADELPHIA,PA. NCI,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A149 EP A149 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900889 ER PT J AU MIDDLETON, LP DURAY, PH MERINO, MJ AF MIDDLETON, LP DURAY, PH MERINO, MJ TI THE HISTOLOGIC SPECTRUM OF HEMANGIOPERICYTOMA - CORRELATION WITH IMMUNOHISTOCHEMISTRY AND PROLIFERATIVE MARKERS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A9 EP A9 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900045 ER PT J AU OSTROWSKI, ML CHIRALA, M MERINO, MJ AF OSTROWSKI, ML CHIRALA, M MERINO, MJ TI TALL CELL VARIANT OF PAPILLARY THYROID-CARCINOMA - A REASSESSMENT AND IMMUNOHISTOCHEMICAL STUDY WITH COMPARISON TO THE USUAL TYPE OF PAPILLARY CARCINOMA OF THE THYROID SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 BAYLOR COLL MED,HOUSTON,TX 77030. METHODIST HOSP,HOUSTON,TX 77030. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A55 EP A55 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900325 ER PT J AU PRYZGODZKI, RM MORAN, C KHAN, MA KOSS, M FINKELSTEIN, SD AF PRYZGODZKI, RM MORAN, C KHAN, MA KOSS, M FINKELSTEIN, SD TI PRIMARY MEDIASTINAL SEMINOMAS - P53 IMMUNOPEROXIDASE AND K-RAS-2 GENE ANALYSIS OF 13 CASES SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 ARMED FORCES INST PATHOL,DIV PULM & MEDIASTINAL,WASHINGTON,DC. NCI,BETHESDA,MD 20892. UNIV PITTSBURGH,MED CTR,PITTSBURGH,PA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A141 EP A141 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900839 ER PT J AU PRZYGODZKI, RM GUINEE, DG TRAVIS, WD KHAN, MA JETT, J COLBY, TV TAZELAAR, H BENNETT, WP SHIELDS, PG PAIROLERO, P TRASTEK, V CAPORASO, NE LIOTTA, LB HARRIS, CC AF PRZYGODZKI, RM GUINEE, DG TRAVIS, WD KHAN, MA JETT, J COLBY, TV TAZELAAR, H BENNETT, WP SHIELDS, PG PAIROLERO, P TRASTEK, V CAPORASO, NE LIOTTA, LB HARRIS, CC TI GSTM1, CYPIA1 AND CYP2E1 GENOTYPES IN SUBJECTS WITH LUNG-CARCINOMA IN THE NCI-MAYO LUNG-CANCER STUDY SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. ARMED FORCES INST PATHOL,DIV PULM PATHOL,WASHINGTON,DC 20306. MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A152 EP A152 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900903 ER PT J AU QUEZADO, M BENJAMIN, DR TSOKOS, M AF QUEZADO, M BENJAMIN, DR TSOKOS, M TI RENAL TUMORS WITH A CLEAR-CELL SARCOMA HISTOLOGY EXHIBITING EWS/FLI-1 FUSION TRANSCRIPTS SIMILAR TO PNET SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NIH,BETHESDA,MD 20892. CHILDRENS HOSP & MED CTR,SEATTLE,WA 98105. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A82 EP A82 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900483 ER PT J AU REID, AH TSAI, MM VENZON, DJ WRIGHT, CF LACK, EE OLEARY, TJ AF REID, AH TSAI, MM VENZON, DJ WRIGHT, CF LACK, EE OLEARY, TJ TI MDM2 AMPLIFICATION, P53 MUTATION, AND P53 ACCUMULATION MALIGNANT FIBROUS HISTIOCYTOMA SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 ARMED FORCES INST PATHOL,WASHINGTON,DC 20306. NCI,BETHESDA,MD 20892. GEORGETOWN UNIV,SCH MED,WASHINGTON,DC. RI Venzon, David/B-3078-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A11 EP A11 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900057 ER PT J AU ROTH, MJ MEDEIROS, LJ ELENITOBAJOHNSON, K JAFFE, ES STETLERSTEVENSON, M AF ROTH, MJ MEDEIROS, LJ ELENITOBAJOHNSON, K JAFFE, ES STETLERSTEVENSON, M TI GRANULOCYTIC SARCOMA - AN IMMUNOHISTOCHEMICAL STUDY SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NATL CANC INST,PATHOL LAB,BETHESDA,MD. RHODE ISL HOSP,DEPT PATHOL,PROVIDENCE,RI 02902. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A119 EP A119 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900710 ER PT J AU SANZORTEGA, J MORO, E STEINBERG, S SANZESPONERA, J MERINO, MJ AF SANZORTEGA, J MORO, E STEINBERG, S SANZESPONERA, J MERINO, MJ TI ANGIOGENESIS AS AN INDEPENDENT PROGNOSTIC FACTOR IN GASTRIC-CARCINOMA SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. UNIV MADRID,HOSP SAN CARLOS,MADRID 3,SPAIN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A68 EP A68 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900400 ER PT J AU SANZORTEGA, J LOPEZ, JA SANZESPONERA, J AF SANZORTEGA, J LOPEZ, JA SANZESPONERA, J TI COMPARATIVE IMMUNOHISTOCHEMISTRY STUDY P53, C-ERBB2, C-MYC AND EGFR AS PROGNOSTIC FACTORS IN STOMACH-CANCER SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. UNIV MADRID,HOSP SAN CARLOS,MADRID 3,SPAIN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A68 EP A68 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900401 ER PT J AU SCHOEDEL, KE GRECO, MA STETLERSTEVENSON, WG PRESENT, D STEINER, GC AF SCHOEDEL, KE GRECO, MA STETLERSTEVENSON, WG PRESENT, D STEINER, GC TI EXPRESSION OF METALLOPROTEINASES AND TISSUE INHIBITORS OF METALLOPROTEINASES IN GIANT-CELL TUMOR OF BONE - AN IMMUNOHISTOCHEMICAL STUDY WITH CLINICAL CORRELATION SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NYU,NEW YORK,NY. HOSP JOINT DIS & MED CTR,NEW YORK,NY 10003. NCI,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A11 EP A11 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900058 ER PT J AU SHERMAN, M STURGEON, S BRINTON, L BERMAN, M MORTEL, R TWIGGS, L BARRETT, R WILBANKS, G AF SHERMAN, M STURGEON, S BRINTON, L BERMAN, M MORTEL, R TWIGGS, L BARRETT, R WILBANKS, G TI ENDOMETRIAL CANCER RISK-FACTORS DIFFER BY HISTOPATHOLOGIC TYPE SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 GEORGE WASHINGTON UNIV,MED CTR,WASHINGTON,DC 20037. NCI,BETHESDA,MD 20892. MILTON S HERSHEY MED CTR,HERSHEY,PA. UNIV CALIF IRVINE,ORANGE,CA 92668. UNIV MINNESOTA,HLTH SCI CTR,MINNEAPOLIS,MN 55455. BOWMAN GRAY SCH MED,WINSTON SALEM,NC. RUSH MED COLL,CHICAGO,IL 60612. RI Brinton, Louise/G-7486-2015 OI Brinton, Louise/0000-0003-3853-8562 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A97 EP A97 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900573 ER PT J AU SOINI, Y BENNETT, WP ISHAK, K HARRIS, CC AF SOINI, Y BENNETT, WP ISHAK, K HARRIS, CC TI P53 MUTATIONS IN PRIMARY HEPATIC ANGIOSARCOMAS NOT ASSOCIATED WITH VINYL-CHLORIDE EXPOSURE SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV OULU,DEPT PATHOL,OULU,FINLAND. NCI,BETHESDA,MD 20892. ARMED FORCES INST PATHOL,WASHINGTON,DC 20306. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A133 EP A133 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900792 ER PT J AU TERUYAFELDSTEIN, J WILSON, WH HARRIS, C STEINBERG, SM WITTES, RE CHABNER, BA BRYANT, GA JAFFE, ES RAFFELD, M AF TERUYAFELDSTEIN, J WILSON, WH HARRIS, C STEINBERG, SM WITTES, RE CHABNER, BA BRYANT, GA JAFFE, ES RAFFELD, M TI P53 EXPRESSION IS ASSOCIATED WITH POOR RESPONSE AND SURVIVAL TO EPOCH CHEMOTHERAPY IN RELAPSED NON-HODGKINS-LYMPHOMA SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,MED BRANCH,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A121 EP A121 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900721 ER PT J AU TERUYAFELDSTEIN, J ZHUANG, ZP MERINO, MJ AF TERUYAFELDSTEIN, J ZHUANG, ZP MERINO, MJ TI ALLELIC LOSS ON CHROMOSOME-17Q21 IN PATIENTS WITH 2 PRIMARY SPORADIC BREAST-OVARIAN-CANCERS SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A27 EP A27 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900153 ER PT J AU TSOKOS, M STEINBERG, SM STOCKER, DM KARAMERIS, A WEXLER, LH AF TSOKOS, M STEINBERG, SM STOCKER, DM KARAMERIS, A WEXLER, LH TI BCL2 MAY BE PREDICTIVE OF SURVIVAL IN PATIENTS WITH PERIPHERAL PRIMITIVE NEUROECTODERMAL TUMORS (PNET) SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A145 EP A145 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900864 ER PT J AU WELLMANN, A CLARK, HM OTSUKI, T JAFFE, ES RAFIELD, M AF WELLMANN, A CLARK, HM OTSUKI, T JAFFE, ES RAFIELD, M TI DETECTION OF THE T(2-5)(P23-Q35) IN CLASSICAL ANAPLASTIC LARGE-CELL LYMPHOMA (ALCL) BY REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION (RT-PCR) SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,HEMATOPATHOL SECT,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A123 EP A123 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900730 ER PT J AU ZHUANG, ZP MERINO, MJ CHUAQUI, R LIOTTA, LA EMMERTBUCK, MR AF ZHUANG, ZP MERINO, MJ CHUAQUI, R LIOTTA, LA EMMERTBUCK, MR TI ALLELIC LOSS ON CHROMOSOME 11Q IN IN-SITU AND INVASIVE SPORADIC HUMAN BREAST-CANCER SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NCI,PATHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP A29 EP A29 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54900168 ER PT J AU MAGID, M KAPLAN, C LOCKSHIN, M AF MAGID, M KAPLAN, C LOCKSHIN, M TI PRENATAL PATHOLOGY IN SYSTEMIC LUPUS-ERYTHEMATOSUS - A PROSPECTIVE-STUDY SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NEW YORK HOSP,CORNELL MED CTR,NEW YORK,NY 10021. SUNY STONY BROOK,STONY BROOK,NY 11794. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1995 VL 72 IS 1 BP P5 EP P5 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA QD549 UT WOS:A1995QD54901034 ER PT B AU GANDJBAKHCHE, AH NOSSAL, R AGAH, R MOTAMEDI, M BONNER, RF AF GANDJBAKHCHE, AH NOSSAL, R AGAH, R MOTAMEDI, M BONNER, RF BE Jacques, SL Katzir, A TI RANDOM WALK METHODOLOGY FOR DETERMINING OPTICAL PROPERTIES OF TISSUE FROM REFLECTION AND TRANSMISSION MEASUREMENTS SO LASER-TISSUE INTERACTION VI, PROCEEDINGS OF SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Laser-Tissue Interaction VI Conference CY FEB 06-09, 1995 CL SAN JOSE, CA SP Soc Photo Opt Instrumentat Engineers DE OPTICAL PROPERTIES OF TISSUE; INTEGRATING SPHERES; INVERSE METHOD C1 NIH,BETHESDA,MD 20892. RI Bonner, Robert/C-6783-2015 NR 0 TC 2 Z9 2 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1738-6 J9 P SOC PHOTO-OPT INS PY 1995 VL 2391 BP 273 EP 283 DI 10.1117/12.209893 PG 11 WC Engineering, Biomedical; Optics SC Engineering; Optics GA BD32D UT WOS:A1995BD32D00028 ER PT J AU HAMDY, N BHATIA, K SHAKER, H KAMEL, A ELMAWLA, NG ABOUENEIN, M YASSIN, D ELSHARKAWY, N MAGRATH, I AF HAMDY, N BHATIA, K SHAKER, H KAMEL, A ELMAWLA, NG ABOUENEIN, M YASSIN, D ELSHARKAWY, N MAGRATH, I TI MOLECULAR EPIDEMIOLOGY OF ACUTE LYMPHOBLASTIC-LEUKEMIA IN EGYPT SO LEUKEMIA LA English DT Article DE ALL; ANTIGEN RECEPTOR GENES; P53 MUTATIONS ID T-CELL RECEPTOR; MYELOID-ANTIGEN EXPRESSION; CHILDHOOD ACUTE-LEUKEMIA; MIXED LINEAGE LEUKEMIA; B-CELL; HEMATOPOIETIC DIFFERENTIATION; HEMATOLOGIC MALIGNANCIES; P53 MUTATIONS; GENE PATTERNS; GAMMA-CHAIN AB We have characterized immunophenotypically defined acute lymphoblastic leukemia (ALL) in Egypt for rearrangements of the antigen receptor genes, and correlated this with rearrangements of ALL-1 and the presence of p53 mutations. Thirty-nine cases were analyzed for rearrangements of the immunoglobulin (Ig) and T-cell receptor (TCR) genes. All precursor B-cell ALLs (12 cases) contained rearranged Ig heavy-chain (JH) region which was biallelic in 92% of these tumors. In addition to JH rearrangements, TCR delta, beta and gamma rearrangements were observed in 80, 40 and 30% of these cases, respectively. TCR genes were invariably rearranged in T-cell ALLs (11 cases). A small fraction (2/11) of T-cell ALL showed concurrent IgJH rearrangement which was monoallelic. Simultaneous rearrangement of IgJH and ICR genes was also observed in both cases of biphenotypic ALL (coexpressing B and T markers). We observed marked heterogeneity in the pattern of rearrangement of antigen receptor genes in mixed-lineage leukemias (ALL coexpressing myeloid-associated markers), including the retention of germline configuration in two cases. Rearrangements of the ALL-1 gene were confined to the leukemias that demonstrated lineage infidelity. Mutations in p53 were infrequent and were present in only three of 47 ALL cases (6%) analyzed; two of these were mixed-lineage leukemias. These results suggest that mixed-lineage and biphenotypic leukemias accumulate pathogenetic lesions that are distinct from B- and T-cell ALL, and that ALL in developing countries includes molecular entities similar to those in developed countries. C1 NCI,LYMPHOMA BIOL SECT,PEDIAT BRANCH,BETHESDA,MD 20892. CAIRO UNIV,NCI,DEPT CLIN PATHOL,CAIRO,EGYPT. OI Kamel, Azza/0000-0002-9744-1851 NR 50 TC 12 Z9 12 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0887-6924 J9 LEUKEMIA JI Leukemia PD JAN PY 1995 VL 9 IS 1 BP 194 EP 202 PG 9 WC Oncology; Hematology SC Oncology; Hematology GA QH987 UT WOS:A1995QH98700030 PM 7845017 ER PT B AU Kaplan, DR AF Kaplan, DR BE Ibanez, CF Hokfelt, T Olson, L Fuxe, K Jornvall, H Ottoson, D TI Signal transduction by Trk receptors SO LIFE AND DEATH IN THE NERVOUS SYSTEM: ROLE OF NEUOTROPHIC FACTORS AND THEIR RECEPTORS SE WENNER-GREN INTERNATIONAL SERIES LA English DT Proceedings Paper CT Conference on the Life and Death in the Nervous System - Role of Neurotrophic Factors and Their Receptors CY SEP 01-02, 1994 CL STOCKHOLM, SWEDEN SP Wenner Gren Fdn C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,EUKARYOT SIGNAL TRANSDUCT SECT,FREDERICK,MD 21702. NR 0 TC 4 Z9 4 U1 0 U2 0 PU PERGAMON PRESS LTD PI OXFORD PA THE BOULEVARD LANGFORD LANE KIDLINGTON, OXFORD, ENGLAND OX5 1GB BN 0-08-042527-5 J9 WENN GR INT PY 1995 VL 67 BP 37 EP 53 PG 17 WC Neurosciences SC Neurosciences & Neurology GA BF61J UT WOS:A1995BF61J00003 ER PT J AU HRYCYNA, CA WAIT, SJ BACKLUND, PS MICHAELIS, S AF HRYCYNA, CA WAIT, SJ BACKLUND, PS MICHAELIS, S TI YEAST STE14 METHYLTRANSFERASE, EXPRESSED AS TRPE-STE14 FUSION PROTEIN IN ESCHERICHIA-COLI, FOR IN-VITRO CARBOXYLMETHYLATION OF PRENYLATED POLYPEPTIDES SO LIPID MODIFICATIONS OF PROTEINS SE METHODS IN ENZYMOLOGY LA English DT Review ID FACTOR MATING PHEROMONE; C-TERMINAL METHYLATION; SACCHAROMYCES-CEREVISIAE; CARBOXYL METHYLTRANSFERASE; FARNESYL CYSTEINE; A-FACTOR; GENE; BINDING; INVITRO; G25K C1 NIMH,GEN COMPARAT BIOCHEM LAB,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,SCH MED,DEPT CELL BIOL & ANAT,BALTIMORE,MD 21205. RP HRYCYNA, CA (reprint author), NCI,CELL BIOL LAB,BETHESDA,MD 20892, USA. FU NIGMS NIH HHS [GM26020, GM41223, GM07185] NR 32 TC 25 Z9 25 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 250 BP 251 EP 266 PG 16 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BD46Q UT WOS:A1995BD46Q00021 PM 7651156 ER PT J AU RANDAZZO, PA KAHN, RA AF RANDAZZO, PA KAHN, RA TI MYRISTOYLATION AND ADP-RIBOSYLATION FACTOR FUNCTION SO LIPID MODIFICATIONS OF PROTEINS SE METHODS IN ENZYMOLOGY LA English DT Review ID GTP-BINDING PROTEINS; GOLGI MEMBRANES; ADENYLATE-CYCLASE; PHOSPHOLIPASE-D; AMINO TERMINUS; RNA-POLYMERASE; CHOLERA-TOXIN; BOVINE BRAIN; BREFELDIN-A; BETA-GAMMA RP RANDAZZO, PA (reprint author), NCI,BIOL CHEM LAB,BETHESDA,MD 20892, USA. NR 42 TC 33 Z9 34 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 250 BP 394 EP 405 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BD46Q UT WOS:A1995BD46Q00031 PM 7651167 ER PT J AU LE, DX THOMA, GR WECHSLER, H AF LE, DX THOMA, GR WECHSLER, H TI CLASSIFICATION OF BINARY DOCUMENT IMAGES INTO TEXTUAL OR NONTEXTUAL DATA BLOCKS USING NEURAL-NETWORK MODELS SO MACHINE VISION AND APPLICATIONS LA English DT Article DE BACK PROPAGATION; DOCUMENT PROCESSING; PROBABILISTIC NETWORKS; RADIAL BASIS FUNCTION; SELF-ORGANIZING FEATURE MAPS; TEXTUAL CLASSIFICATION ID SEGMENTATION AB This paper describes a new method for the classification of binary document images as textual or nontextual data blocks using neural network models. Binary document images are first segmented into blocks by the constrained run-length algorithm (CRLA). The component-labeling procedure is used to label the resulting blocks. The features for each block, calculated from the coordinates of its extremities, are then fed into the input layer of a neural network for classification. Four neural networks were considered, and they include back propagation (BP), radial basis functions (RBF), probabilistic neural network (PNN), and Kohonen's self-organizing feature maps (SOFMs). The performance and behavior of these neural network models are analyzed and compared in terms of training times, memory requirements, and classification accuracy. The experiments carried out on a variety of medical journals show the feasibility of using the neural network approach for textual block classification and indicate that in terms of both accuracy and training time RBF should be preferred. C1 GEORGE MASON UNIV,DEPT COMP SCI,FAIRFAX,VA 22030. RP LE, DX (reprint author), NATL LIB MED,LISTER HILL NATL CTR BIOMED COMMUN,8600 ROCKVILLE PIKE BLVD,BLDG 38A,MS 55,BETHESDA,MD 20894, USA. NR 17 TC 9 Z9 9 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0932-8092 J9 MACH VISION APPL JI Mach. Vis. Appl. PY 1995 VL 8 IS 5 BP 289 EP 304 PG 16 WC Computer Science, Artificial Intelligence; Computer Science, Cybernetics; Engineering, Electrical & Electronic SC Computer Science; Engineering GA TE403 UT WOS:A1995TE40300004 ER PT J AU POSSE, S TEDESCHI, G RISINGER, R OGG, R LEBIHAN, D AF POSSE, S TEDESCHI, G RISINGER, R OGG, R LEBIHAN, D TI HIGH-SPEED H-1 SPECTROSCOPIC IMAGING IN HUMAN BRAIN BY ECHO-PLANAR SPATIAL-SPECTRAL ENCODING SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE PROTON SPECTROSCOPY; SPECTROSCOPIC IMAGING; ECHO PLANAR ENCODING; HUMAN BRAIN ID CHEMICAL-SHIFT; NMR-SPECTROSCOPY; TRAINS AB We introduce a fast and robust spatial-spectral encoding method, which enables acquisition of high resolution short echo time (13 ms) proton spectroscopic images from human brain with acquisition times as short as 64 s when using surface coils. The encoding scheme, which was implemented on a clinical 1.5 Tesla whole body scanner, is a modification of an echo-planar spectroscopic imaging method originally proposed by Mansfield Magn. Reson. Med. 1, 370-386 (1984), and utilizes a series of read-out gradients to simultaneously encode spatial and spectral information. Superficial lipid signals are suppressed by a novel double outer volume suppression along the contours of the brain. The spectral resolution and the signal-to-noise per unit time and unit volume from resonances such as N-acetyl aspartate, choline, creatine, and inositol are comparable with those obtained with conventional methods. The short encoding time of this technique enhances the flexibility of in vivo spectroscopic imaging by reducing motion artifacts and allowing acquisition of multiple data sets with different parameter settings. C1 NINCDS, WARREN GRANT MAGNUSON CLIN CTR, DEPT DIAGNOST RADIOL, BETHESDA, MD 20892 USA. NINCDS, NEUROIMAGING BRANCH, BETHESDA, MD 20892 USA. NIMH, CLIN PHARMACOL SECT, BETHESDA, MD 20892 USA. ST JUDE CHILDRENS RES HOSP, MEMPHIS, TN 38105 USA. NR 28 TC 174 Z9 176 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0740-3194 J9 MAGN RESON MED JI Magn. Reson. Med. PD JAN PY 1995 VL 33 IS 1 BP 34 EP 40 DI 10.1002/mrm.1910330106 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA PZ808 UT WOS:A1995PZ80800005 PM 7891533 ER PT J AU GILLIS, P PETO, S MOINY, F MISPELTER, J CUENOD, CA AF GILLIS, P PETO, S MOINY, F MISPELTER, J CUENOD, CA TI PROTON TRANSVERSE NUCLEAR MAGNETIC-RELAXATION IN OXIDIZED BLOOD - A NUMERICAL APPROACH SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE TRANSVERSE RELAXATION; OXIDIZED BLOOD; COMPUTER SIMULATION ID SENSORY STIMULATION; WATER; BRAIN; MRI; DEOXYHEMOGLOBIN; ERYTHROCYTES; DEPENDENCE; DIFFUSION; CONTRAST; FIELDS AB When red blood cells are deoxygenated, hemoglobin, which is then transformed into deoxyhemoglobin or methemoglobin, becomes paramagnetic, The transverse nuclear magnetic relaxation rate of water protons is considerably enhanced by this chemical transformation. A general agreement exists about the origin of the phenomenon-local field inhomogeneities induced by paramagnetic centers randomly distributed within the cell-but the localization of the region that dominates the relaxation is unclear. We addressed this problem with a computer simulation devoted to the determination of transverse magnetic relaxation of water protons in the presence of superparamagnetic MRI contrast agent candidates. The simulation confirms an earlier experimental result that shares equitably the responsibility for the observed relaxation between intracellular and extracellular water. C1 CTR UNIV ORSAY,INST CURIE BIOL,INSERM,U350,F-91405 ORSAY,FRANCE. NIH,CTR CLIN,BETHESDA,MD 20892. RP GILLIS, P (reprint author), UNIV MONS HAINAUT,FAC MED,AVE CHAMP MARS 24,B-7000 MONS,BELGIUM. NR 24 TC 44 Z9 44 U1 0 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD JAN PY 1995 VL 33 IS 1 BP 93 EP 100 DI 10.1002/mrm.1910330114 PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA PZ808 UT WOS:A1995PZ80800013 PM 7891542 ER PT J AU DUYN, JH FRANK, JA MOONEN, CTW AF DUYN, JH FRANK, JA MOONEN, CTW TI INCORPORATION OF LACTATE MEASUREMENT IN MULTI-SPIN-ECHO PROTON SPECTROSCOPIC IMAGING SO MAGNETIC RESONANCE IN MEDICINE LA English DT Note DE MR PROTON SPECTROSCOPY; MR PROTON SPECTROSCOPIC IMAGING; LACTATE EDITING; LIPID SUPPRESSION ID HOMONUCLEAR POLARIZATION TRANSFER; HUMAN-BRAIN; UNEQUIVOCAL DETERMINATION; NMR-SPECTROSCOPY; PULSE SEQUENCE; INVIVO; COHERENCE; METABOLITES; ORDER AB An improved multi-slice, multi-spin-echo proton spectroscopic imaging method for human brain is presented. The technique allows the reconstruction of lactate images, along with choline plus creatine, N-acetylaspartate, and lipid images from one single data set processed in three separate ways. The discrimination between resonances of lipid protons and lactate methyl protons is based on homonuclear spin-spin coupling. The reliability of the separation of the lipid and lactate contribution depends on the T-2 of the lipid resonances. Measurements were performed on a standard 1.5 Tesla clinical scanner on healthy Volunteers and a patient with high grade CNS lymphoma, demonstrating the ability to obtain high quality metabolite maps within 11 min. C1 NIH,NATL CTR RES RESOURCES,IN VIVO NMR RES CTR,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NIH,OFF INTRAMURAL RES,DIAGNOST RADIOL RES LAB,BETHESDA,MD 20892. RI Duyn, Jozef/F-2483-2010; Moonen, Chrit/K-4434-2016 OI Moonen, Chrit/0000-0001-5593-3121 NR 30 TC 29 Z9 29 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD JAN PY 1995 VL 33 IS 1 BP 101 EP 107 DI 10.1002/mrm.1910330115 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA PZ808 UT WOS:A1995PZ80800014 PM 7891522 ER PT J AU PAVAN, WJ LIDDELL, RA WRIGHT, A THIBAUDEAU, G MATTESON, PG MCHUGH, KM SIRACUSA, LD AF PAVAN, WJ LIDDELL, RA WRIGHT, A THIBAUDEAU, G MATTESON, PG MCHUGH, KM SIRACUSA, LD TI A HIGH-RESOLUTION LINKAGE MAP OF THE LETHAL SPOTTING LOCUS - A MOUSE MODEL FOR HIRSCHSPRUNG DISEASE SO MAMMALIAN GENOME LA English DT Article ID PRESUMPTIVE AGANGLIONIC BOWEL; NEURAL CREST CELLS; MUTANT MICE; ASSOCIATION; MEGACOLON; COLONIZE AB Mice homozygous for the lethal spotting (ls) mutation exhibit aganglionic megacolon and a white spotted coat owing to a lack of neural crest-derived enteric ganglia and melanocytes. The ls mutation disrupts the migration, differentiation, or survival of these neural crest lineages during mammalian development. A human congenital disorder, Hirschsprung disease (HSCR), is also characterized by aganglionic megacolon of the distal bowel and can be accompanied by hypopigmentation of the skin. HSCR has been attributed to multiple loci acting independently or in combination. The ls mouse serves as one animal model for HSCR, and the ls gene may represent one of the loci responsible for some cases of HSCR in humans. This study uses 753 N-2 progeny from a combination of three intersubspecific backcrosses to define the molecular genetic linkage map of the Es region and to provide resources necessary for positional cloning. Similar to some cases of HSCR, the ls mutation acts semidominantly, its phenotypic effects dependent upon the presence of modifier genes segregating in the crosses. We have now localized the ls mutation to a 0.8-cM region between the D2Mit113 and D2Mit73/D2Mit174 loci. Three genes, endothelin-3 (Edn3), guanine nucleotide-binding protein ex-stimulating polypeptide 1 (Gnas), and phosphoenolpyruvate carboxykinase (Pck1) were assessed as candidates for the ls mutation. Only Edn3 and Gnas did not recombine with the ls mutation. Mutational analysis of the Edn3 and Gnas genes will determine whether either gene is responsible for the neural crest deficiencies observed in ls/ls mice. C1 THOMAS JEFFERSON UNIV,JEFFERSON CANC INST,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19107. NIH,NATL CTR HUMAN GENOME RES,GENET DIS RES LAB,BETHESDA,MD 20892. MISSISSIPPI STATE UNIV,DEPT BIOL SCI,MISSISSIPPI STATE,MS 39762. HOWARD HUGHES MED INST,PRINCETON,NJ 08534. DEPT MOLEC BIOL,PRINCETON,NJ 08534. THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT ANAT PATHOL & CELL BIOL,PHILADELPHIA,PA 19107. RI McHugh, Kirk/E-3640-2011 FU NICHD NIH HHS [HD00996, HD27252]; NIDDK NIH HHS [DK45717, R01 DK055791] NR 45 TC 13 Z9 13 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1995 VL 6 IS 1 BP 1 EP 7 DI 10.1007/BF00350885 PG 7 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA QB671 UT WOS:A1995QB67100001 PM 7719019 ER PT J AU WINN, RN VANBENEDEN, RJ BURKHART, JG AF WINN, RN VANBENEDEN, RJ BURKHART, JG TI TRANSFER, METHYLATION AND SPONTANEOUS MUTATION FREQUENCY OF PHI-X174AM3CS70 SEQUENCES IN MEDAKA (ORYZIAS-LATIPES) AND MUMMICHOG (FUNDULUS-HETEROCLITUS) - IMPLICATIONS FOR GENE-TRANSFER AND ENVIRONMENTAL MUTAGENESIS IN AQUATIC SPECIES SO MARINE ENVIRONMENTAL RESEARCH LA English DT Article ID TRANSGENIC FISH; DNA METHYLATION; BRACHYDANIO-RERIO; WINTER FLOUNDER; RAINBOW-TROUT; RAS ONCOGENE; BASE PAIR; EXPRESSION; CELLS; ZEBRAFISH AB This study describes the production of transgenic medaka (Oryzias latipes) and mummichog (Fundulus heteroclitus) containing multiple copies of the bacteriophage Phi X174am3cs70. This work is an initial approach for measuring mutations in aquatic species using the same gene target sequence in fish and laboratory mammals, The Phi X174 sequence is unique in that there is no detectable homology with chromosomal DNA of medaka, mummichog or mice. The authors have compared cytoplasmic injection of 1-2 cell embryos with linear single copy and catenated constructs of the phage DNA. The catenated construct results in greater efficiency of gene transfer for both species in terms of copies per cell, Analyses of DNA from founder transgenic fish with methylation sensitive (HpaII) and methylation insensitive (MspI) restriction enzyme isoschizmers indicates CpG methylation of the integrated Phi X174 sequence, This study also demonstrates the efficient rescue of live phage from the chromosomal DNA of founder fish in sufficient numbers to determine a spontaneous mutation frequency for reversion of am3. A pooled sample of 20 mu g DNA from four fish yielded 1.09 x 10(7) progeny phage with a spontaneous mutation frequency of 1.83 x 10(-7). This spontaneous mutation frequency is similar to the spontaneous frequency for the same gene indictor recovered from transgenic mice. These results demonstrate that fish containing multiple copies of Phi X174 can be produced with no obvious detrimental effects and that the overall approach may be useful in basic and applied studies of environmental mutagenesis. C1 NIEHS,ENVIRONM TOXICOL PROGRAM,RES TRIANGLE PK,NC 27709. UNIV MAINE,DEPT ZOOL,ORONO,ME 04469. NR 67 TC 18 Z9 19 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0141-1136 J9 MAR ENVIRON RES JI Mar. Environ. Res. PY 1995 VL 40 IS 3 BP 247 EP 265 DI 10.1016/0141-1136(94)00144-E PG 19 WC Environmental Sciences; Marine & Freshwater Biology; Toxicology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Toxicology GA RR906 UT WOS:A1995RR90600002 ER PT B AU CARTER, CS GRESS, R KLEIN, HG AF CARTER, CS GRESS, R KLEIN, HG BE Lasky, LC Warkentin, PI TI ROUTINE METHODS FOR PREPARING HEMATOPOIETIC PROGENITOR CELL COMPONENTS SO MARROW AND STEM CELL PROCESSING FOR TRANSPLANTATION LA English DT Proceedings Paper CT Conference on Marrow and Stem Cell Processing for Transplantation CY MAR 07-09, 1993 CL TAMPA, FL SP AMER ASSOC BLOOD BANKS C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT TRANSFUS MED,SPECIAL SERV LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 BN 1-56395-042-1 PY 1995 BP 51 EP 68 PG 18 WC Cell Biology; Hematology; Transplantation SC Cell Biology; Hematology; Transplantation GA BD11B UT WOS:A1995BD11B00004 ER PT S AU KARANIAN, JW SALEM, N AF KARANIAN, JW SALEM, N BE Schror, K PaceAsciak, CR TI Hydroxylated 22-carbon fatty acids in platelet and vascular smooth muscle function: Interference with TXA2/PGH2 receptors SO MEDIATORS IN THE CARDIOVASCULAR SYSTEM: REGIONAL ISCHEMIA SE AGENTS AND ACTIONS SUPPLEMENTS LA English DT Proceedings Paper CT 1st Interdisciplinary Symposium on Mediators in the Cardiovascular System CY JUN 02-05, 1994 CL MALTA RP NIAAA,DICBR,MEMBRANE BIOCHEM & BIOPHYS LAB,BLDG 10 3C-102,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA PO BOX 133, CH-4010 BASEL, SWITZERLAND SN 0379-0363 BN 3-7643-5130-6 J9 AGENT ACTION SUPPL PY 1995 VL 45 BP 39 EP 45 PG 7 WC Biochemistry & Molecular Biology; Cardiac & Cardiovascular Systems SC Biochemistry & Molecular Biology; Cardiovascular System & Cardiology GA BC27P UT WOS:A1995BC27P00006 PM 7717199 ER PT J AU YANAGISHITA, M AF YANAGISHITA, M TI CELL-SURFACE HEPARAN-SULFATE PROTEOGLYCANS - MODULATOR OF GROWTH-FACTORS, CYTOKINES AND VIRAL-INFECTION SO MEDICINA-BUENOS AIRES LA English DT Article; Proceedings Paper CT XL Scientific Reunion of Argentine-Society-of-Clinical-Investigation CY NOV 08-12, 1995 CL MAR DEL PLATA, ARGENTINA SP Argentine Soc Clin Investigat RP YANAGISHITA, M (reprint author), NIDR,BONE RES BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MEDICINA (BUENOS AIRES) PI BUENOS AIRES PA DONATO ALVAREZ 3150, 1427 BUENOS AIRES, ARGENTINA SN 0025-7680 J9 MEDICINA-BUENOS AIRE JI Med.-Buenos Aires PY 1995 VL 55 IS 5 BP 615 EP 616 PN 2 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA TF662 UT WOS:A1995TF66200014 ER PT J AU PEI, XF GREIG, NH BI, S BROSSI, A TOOME, V AF PEI, XF GREIG, NH BI, S BROSSI, A TOOME, V TI INHIBITION OF HUMAN ACETYLCHOLINESTERASE BY UNNATURAL (+)-(3AR)-N-1-NORPHYSOSTIGMINE AND ARYLCARBAMATE ANALOGS SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID CARBAMATE ANALOGS; ANTICHOLINESTERASE ACTIVITY AB (+)-(3aR)-N-1-Norphysostigmine (3b) and 3 arylcarbamate analogues (4b-6b) were prepared. They are less active inhibitors of human acetyl- and butyrylcholinesterase than their (-)-(3aS)-enantiomers but retained considerable activity. C1 NIA,NEUROSCI LAB,BETHESDA,MD 20892. GEORGETOWN UNIV,DEPT CHEM,WASHINGTON,DC 20057. HOFFMAN LA ROCHE,NUTLEY,NJ. NR 14 TC 9 Z9 9 U1 0 U2 0 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1995 VL 5 IS 4 BP 265 EP 270 PG 6 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA RC780 UT WOS:A1995RC78000003 ER PT J AU FRYER, RI WANG, CG CHIA, NC BASILE, AS GU, ZQ SKOLNICK, P AF FRYER, RI WANG, CG CHIA, NC BASILE, AS GU, ZQ SKOLNICK, P TI BENZODIAZEPINE-RECEPTOR (BZR) LIGANDS - THE CONFORMATIONAL FIT OF AGONIST-BETA-CARBOLINES TO AN IMIDAZOBENZODIAZEPINE TEMPLATE SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID INVERSE AGONISTS; PHARMACOPHORE; DERIVATIVES; ANTAGONISTS; BINDING; ESTER AB This work describes the rationale for the design and synthesis as well as the in vitro biological evaluation of three novel analogs of ethyl 6-benzyloxy-4-methoxymethyl-beta-carboline-3-carboxylate (ZK93423), a BZR agonist ligand. Previous computer graphics analyses had shown that it should be possible to conformationally constrain ZK93423 to a three dimensional fit within the volume of an imidazobenzodiazepine BZR agonist. The novel analogs have substituents known to reduce or abolish the affinity of beta-carboline inverse agonists, but predictably should be allowed for agonists, if our previously published model for receptor agonist binding to the BZR is valid. C1 RUTGERS STATE UNIV,DEPT CHEM,NEWARK,NJ 07102. NIDDK,NEUROSCI LAB,BETHESDA,MD 20892. NR 28 TC 1 Z9 1 U1 0 U2 1 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1995 VL 5 IS 4 BP 296 EP 307 PG 12 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA RC780 UT WOS:A1995RC78000006 ER PT J AU PEI, XF GREIG, NH BI, S BROSSI, A AF PEI, XF GREIG, NH BI, S BROSSI, A TI PREPARATION AND SELECTIVE-INHIBITION OF HUMAN BUTYRYLCHOLINESTERASE BY N-1-PHENETHYLNORPHYSOSTIGMINE ANALOGS SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article ID PHYSOSTIGMINE AB N-1-Phenethylnorphysostigmine (13) and 3 arylcarbamate analogues (14-16) were prepared by a novel route. These compounds are less active inhibitors of human acetylcholinesterase (AChE) but more potent and selective inhibitors of human butyryIcholinesterase (BChE) than their corresponding N-1-methyl and N-1-H analogues. C1 GEORGETOWN UNIV,DEPT CHEM,WASHINGTON,DC 20057. NIA,NEUROSCI LAB,BETHESDA,MD 20892. NR 15 TC 10 Z9 10 U1 0 U2 0 PU BIRKHAUSER BOSTON INC PI CAMBRIDGE PA 675 MASSACHUSETTS AVE, CAMBRIDGE, MA 02139 SN 1054-2523 J9 MED CHEM RES JI Med. Chem. Res. PY 1995 VL 5 IS 6 BP 455 EP 461 PG 7 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA RM621 UT WOS:A1995RM62100007 ER PT J AU GREIG, NH PEI, XF SONCRANT, TT INGRAM, DK BROSSI, A AF GREIG, NH PEI, XF SONCRANT, TT INGRAM, DK BROSSI, A TI PHENSERINE AND RING-C HETEROANALOGS - DRUG CANDIDATES FOR THE TREATMENT OF ALZHEIMERS-DISEASE SO MEDICINAL RESEARCH REVIEWS LA English DT Review ID PLASMA PHYSOSTIGMINE CONCENTRATIONS; INDUCED LEARNING IMPAIRMENT; 14-UNIT T-MAZE; CHOLINESTERASE INHIBITOR; HEPTYL-PHYSOSTIGMINE; ORAL PHYSOSTIGMINE; LONG-TERM; ANTICHOLINESTERASE ACTIVITY; CHOLINERGIC SYSTEM; CARBAMATE ANALOGS C1 NIA,GERONTOL RES CTR,NATAN W SHOCK LABS,MOLEC PHYSIOL & GENET SECT,BETHESDA,MD 21224. GEORGETOWN UNIV,DEPT CHEM,WASHINGTON,DC 20057. RP GREIG, NH (reprint author), NIA,NEUROSCI LAB,BLDG 10,ROOM 6C 103,BETHESDA,MD 20892, USA. NR 107 TC 128 Z9 129 U1 1 U2 6 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0198-6325 J9 MED RES REV JI Med. Res. Rev. PD JAN PY 1995 VL 15 IS 1 BP 3 EP 31 DI 10.1002/med.2610150103 PG 29 WC Chemistry, Medicinal; Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QH454 UT WOS:A1995QH45400002 PM 7898167 ER PT B AU Hearing, VJ Kobayashi, T Urabe, K Potterf, B Kameyama, K AF Hearing, VJ Kobayashi, T Urabe, K Potterf, B Kameyama, K BE Zeise, L Chedekel, MR Fitzpatrick, TB TI The characteristics of biological melanins are influenced at multiple points in the melanogenic pathway SO MELANIN: ITS ROLE IN HUMAN PHOTOPROTECTION LA English DT Proceedings Paper CT Symposium on Melanin - Its Role in Human Photoprotection CY MAR 11-12, 1994 CL WASHINGTON, DC SP Adv Polym Syst Inc, Lancaster Grp, Biosource Genet Corp, DUSA Pharm Inc, Estee Lauder C1 NATL INST HLTH,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU VALDENMAR PUBLISHING CO PI OVERLAND PK PA 9812 W 121ST TERRACE, OVERLAND PK, KS 66213-1677 BN 0-9632105-2-1 PY 1995 BP 117 EP 124 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Dermatology SC Biochemistry & Molecular Biology; Cell Biology; Dermatology GA BE87M UT WOS:A1995BE87M00013 ER PT J AU KLEIN, H ABASSI, Z KEISER, HR AF KLEIN, H ABASSI, Z KEISER, HR TI EFFECTS OF ANGIOTENSIN-II AND PHENYLEPHRINE ON URINARY ENDOTHELIN IN NORMAL FEMALE VOLUNTEERS SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID ATRIAL-NATRIURETIC-PEPTIDE; MEDULLARY COLLECTING DUCT; IMMUNOREACTIVE ENDOTHELIN; VASOCONSTRICTOR PEPTIDE; NEUTRAL ENDOPEPTIDASE; EPITHELIAL-CELLS; RAT; SECRETION; INHIBITION; MECHANISM AB Endothelin (ET) is a 21-amino acid peptide produced and secreted mainly by endothelial cells. Small amounts of ET are found in plasma, whereas large amounts are present in the urine. Despite the abundance of ET in the kidneys and urine, little is known about its regulation and clinical significance. The present study was designed to examine the effects of angiotensin II (Ang II) and phenylephrine (Phe) on the excretion of ET in normal female volunteers. Ang II and Phe were infused for 1 hour each and titrated to increase the mean arterial pressure by 20 mm Hg. There was a 60-minute recovery period before the second drug, and the order of the drugs was randomized. Infusion of Phe induced mild diuresis and natriuresis, which were associated with a significant increase in the excretion of ET. In addition, Phe significantly increased plasma atrial natriuretic factor (ANF). In contrast, infusion of equipressor doses of Ang II decreased urinary sodium excretion and did not significantly alter the excretion of ET. Moreover, Ang II induced only a small and nonsignificant increase in plasma ANF. These results demonstrate that (1) physiological doses of Ang II do not affect excretion of either ET or ANF; (2) Phe markedly increased the excretion of ET and ANF, independently of its effect on blood pressure; and (3) neither agent changed plasma ET, but Phe increased plasma ANF. This is a US government work. There are no restrictions on its use. RP KLEIN, H (reprint author), NHLBI,HYPERTENS ENDOCRINE BRANCH,BLDG 10,ROOM 8C-103,BETHESDA,MD 20892, USA. NR 30 TC 6 Z9 6 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD JAN PY 1995 VL 44 IS 1 BP 115 EP 118 DI 10.1016/0026-0495(95)90297-X PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QA741 UT WOS:A1995QA74100019 PM 7854155 ER PT J AU OSTROWSKI, LE RANDELL, SH CLARK, AB GRAY, TE NETTESHEIM, P AF OSTROWSKI, LE RANDELL, SH CLARK, AB GRAY, TE NETTESHEIM, P TI CILIOGENESIS OF RAT TRACHEAL EPITHELIAL-CELLS IN-VITRO SO METHODS IN CELL BIOLOGY, VOL 47 SE METHODS IN CELL BIOLOGY LA English DT Review ID CULTURE C1 UNIV N CAROLINA,CTR CYST FIBROSIS PULM RES & TREATMENT,DEPT MED,CHAPEL HILL,NC 27599. RP OSTROWSKI, LE (reprint author), NIEHS,PULM PATHOBIOL LAB,AIRWAY CELL BIOL GRP,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 15 TC 13 Z9 13 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL PY 1995 VL 47 BP 57 EP 63 DI 10.1016/S0091-679X(08)60791-8 PG 7 WC Cell Biology SC Cell Biology GA BE19X UT WOS:A1995BE19X00010 PM 7476546 ER PT J AU Krause, M AF Krause, M TI Transcription and translation SO METHODS IN CELL BIOLOGY, VOL 48 SE METHODS IN CELL BIOLOGY LA English DT Review ID NEMATODE CAENORHABDITIS-ELEGANS; HETEROCHRONIC GENE LIN-14; SEX-DETERMINING GENE; HEAT-SHOCK GENE; NUCLEAR RIBONUCLEOPROTEIN-PARTICLES; MAJOR DEVELOPMENTAL TRANSITION; MESSENGER-RNA SYNTHESIS; EARLY XENOPUS-EMBRYOS; ZINC-FINGER PROTEINS; SPLICED LEADER RNA RP Krause, M (reprint author), NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. OI Krause, Michael/0000-0001-6127-3940 NR 146 TC 25 Z9 25 U1 1 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL PY 1995 VL 48 BP 483 EP 512 PG 30 WC Biochemical Research Methods; Cell Biology; Developmental Biology SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology GA BE40R UT WOS:A1995BE40R00020 PM 8531739 ER PT J AU Krause, M AF Krause, M TI Techniques for analyzing transcription and translation SO METHODS IN CELL BIOLOGY, VOL 48 SE METHODS IN CELL BIOLOGY LA English DT Review ID NEMATODE CAENORHABDITIS-ELEGANS; SEX-DETERMINATION GENE; TRANS-SPLICED LEADER; C-ELEGANS; MESSENGER-RNAS; MIDBLASTULA TRANSITION; COLLAGEN GENES; EXPRESSION; CLONING; PROTEIN RP Krause, M (reprint author), NIDDKD,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. OI Krause, Michael/0000-0001-6127-3940 NR 52 TC 21 Z9 21 U1 0 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL PY 1995 VL 48 BP 513 EP 529 PG 17 WC Biochemical Research Methods; Cell Biology; Developmental Biology SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology GA BE40R UT WOS:A1995BE40R00021 PM 8531740 ER PT J AU TASA, G UUSKULA, M HIRVONEN, A MIKELSAAR, AV AF TASA, G UUSKULA, M HIRVONEN, A MIKELSAAR, AV TI OPTIMIZATION OF PCR TO YIELD SUCCESSFUL AMPLIFICATION FROM HEPARIN-CONTAMINATED DNA SO METHODS IN MOLECULAR AND CELLULAR BIOLOGY LA English DT Note ID POLYMERASE CHAIN-REACTION; VIRUS AB In clinical laboratories blood samples are often collected in the presence of heparin as an anticoagulant. Heparin, bound to DNA during the extraction process, has an inhibitory effect on the polymerase chain reaction (PCR). In this study we report a simple procedure that allows heparin-contaminated DNA to be used as a template in an amplification reaction. The PCR protocol presented involves reduction of the DNA concentration added to the PCR mixture and an increase in Taq-polymerase concentration as well as the number of amplification cycles. In comparison with other current techniques, our approach provides a rapid and inexpensive method of utilizing DNA from heparinized blood for PCR. (C) 1995 Wiley-Liss, Inc. C1 TARTU STATE UNIV,INST GEN & MOLEC PATHOL,DEPT HUMAN BIOL & GENET,TARTU,ESTONIA. INST OCCUPAT HLTH,DEPT IND HYG & TOXICOL,SF-00250 HELSINKI,FINLAND. NIEHS,BIOCHEM RISK ANAL LAB,RES TRIANGLE PK,NC 27711. NR 8 TC 2 Z9 2 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0898-7750 J9 METHOD MOL CELL BIOL JI Methods Mol. Cell. Biol. PY 1995 VL 5 IS 2 BP 122 EP 124 PG 3 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA RB026 UT WOS:A1995RB02600006 ER PT B AU Appella, E Sakaguchi, K Sakamoto, H Lewis, MS Omichinski, JG Gronenborn, AM Clore, GM Anderson, CW AF Appella, E Sakaguchi, K Sakamoto, H Lewis, MS Omichinski, JG Gronenborn, AM Clore, GM Anderson, CW BE Atassi, MZ Appella, E TI The p53 tumor suppressor protein - Biophysical characterization of the carboxy-terminal oligomerization domain SO METHODS IN PROTEIN STRUCTURE ANALYSIS LA English DT Proceedings Paper CT 10th International Conference on Methods in Protein Structure Analysis CY SEP 08-13, 1994 CL SNOWBIRD, UT SP Int Symp Immunobiol Protein & Peptides Inc C1 NCI,NIH,CELL BIOL LAB,BETHESDA,MD 20892. RI Sakamoto, Hiroshi/A-3181-2011 NR 0 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45124-7 PY 1995 BP 407 EP 418 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA BE70Z UT WOS:A1995BE70Z00036 ER PT B AU Clore, GM Gronenborn, AM AF Clore, GM Gronenborn, AM BE Atassi, MZ Appella, E TI Three- and four-dimensional heteronuclear NMR SO METHODS IN PROTEIN STRUCTURE ANALYSIS LA English DT Proceedings Paper CT 10th International Conference on Methods in Protein Structure Analysis CY SEP 08-13, 1994 CL SNOWBIRD, UT SP Int Symp Immunobiol Protein & Peptides Inc C1 NIDDKD,NIH,PHYS CHEM LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45124-7 PY 1995 BP 493 EP 503 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA BE70Z UT WOS:A1995BE70Z00045 ER PT B AU FREDRICKSON, DS AF FREDRICKSON, DS BE Rogers, DE Ginzberg, E TI Federal support of biomedical research, 1945-1993 SO METROPOLITAN ACADEMIC MEDICAL CENTER: ITS ROLE IN AN ERA OF TIGHT MONEY AND CHANGING EXPECTATIONS LA English DT Proceedings Paper CT Cornell-University-Medical-College 9th Conference on Health Policy - Metropolitan Academic Medical Center: Its Role in an Era of Tight Money and Changing Expectations CY DEC 09-10, 1993 CL NEW YORK, NY SP Cornell Univ Med Coll C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WESTVIEW PRESS PI BOULDER PA 5500 CENTRAL AVENUE, BOULDER, CO 80301-2877 BN 0-8133-2574-9 PY 1995 BP 81 EP 97 PG 17 WC Health Policy & Services SC Health Care Sciences & Services GA BE07U UT WOS:A1995BE07U00010 ER PT B AU LEWIS, EN AF LEWIS, EN BE Etz, ES TI Applications of a novel infrared interferometric technique for rapidly performing simultaneous chemical state imaging and spectroscopy. SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 103 EP 104 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00045 ER PT B AU SWYT, CR AF SWYT, CR BE Etz, ES TI Comparison of simplex and multiple linear regression peak fitting using generated spectra SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NATL INST HLTH,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 207 EP 208 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00093 ER PT B AU NEWBURY, DE LEAPMAN, RD AF NEWBURY, DE LEAPMAN, RD BE Etz, ES TI The analysis of nanoscale particles by analytical electron microscopy SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NATL INST HLTH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NIST,DIV SURFACE & MICROANAL SCI,GAITHERSBURG,MD 20899. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 231 EP 232 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00104 ER PT B AU LEAPMAN, RD SUN, SQ AF LEAPMAN, RD SUN, SQ BE Etz, ES TI Detection of hydrogen in irradiated, frozen hydrated organic materials by EELS SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 2 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 307 EP 308 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00140 ER PT B AU LEAPMAN, RD ANDREWS, SB AF LEAPMAN, RD ANDREWS, SB BE Etz, ES TI Improved method for quantifying EELS difference spectra from biological specimens SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 331 EP 332 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00151 ER PT B AU SUN, SQ SHI, SL LEAPMAN, RD AF SUN, SQ SHI, SL LEAPMAN, RD BE Etz, ES TI Electron energy loss spectra from biological compounds SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 333 EP 334 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00152 ER PT B AU SHI, SL SUN, SQ BOWERS, B LEAPMAN, RD AF SHI, SL SUN, SQ BOWERS, B LEAPMAN, RD BE Etz, ES TI Measurement of ion concentrations in very dilute cellular compartment by analytical electron microscopy SO MICROBEAM ANALYSIS 1995: PROCEEDINGS OF THE 29TH ANNUAL CONFERENCE OF THE MICROBEAM ANALYSIS SOCIETY SE MICROBEAM ANALYSIS LA English DT Proceedings Paper CT 29th Annual Conference of the Microbeam-Analysis-Society CY AUG 06-11, 1995 CL BRECKENRIDGE, CO SP Microbeam Anal Soc C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU V C H PUBLISHERS PI NEW YORK PA 220 E 23RD ST, SUITE 909, NEW YORK, NY 10010 BN 1-56081-919-7 J9 MICROB ANAL PY 1995 BP 335 EP 336 PG 2 WC Microscopy; Spectroscopy SC Microscopy; Spectroscopy GA BE26U UT WOS:A1995BE26U00153 ER PT J AU DOHURA, K PERRYMAN, S RACE, R CHESEBRO, B AF DOHURA, K PERRYMAN, S RACE, R CHESEBRO, B TI IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN SCRAPIE-INFECTED MOUSE NEUROBLASTOMA-CELLS SO MICROBIAL PATHOGENESIS LA English DT Article DE SCRAPIE; NEUROBLASTOMA; DIFFERENTIAL SCREENING; NORTHERN BLOT ID ALZHEIMERS-DISEASE; PROTEIN; PRION; BRAIN; PRP; RESISTANT; MICE; INFECTIVITY; ASTROCYTES; PLAQUES AB In vitro cDNA libraries from scrapie-infected and non-infected murine neuroblastoma cell lines were screened with cDNA probes derived by subtractive hybridization from scrapie-infected and uninfected cells to identify genes with altered expression associated with scrapie infection. Eleven independent recombinant clones, whose expression was either increased or decreased in scrapie-infected cells, were identified. Expression of these genes was also analyzed in a panel of scrapie-infected and non-infected cell lines. Five genes had altered mRNA expression in most scrapie-infected neuroblastoma cell clones compared to non-infected clones. These genes were chromogranin B, intracisternal-A particle envelope, ornithine decarboxylase antizyme, heat shock protein 70 and one unidentified gene. None of these alterations in gene expression was uniquely scrapie-specific; however, the skewed association of increased expression with scrapie infection suggested that a causal relationship might exist between scrapie infection and altered expression of these genes in mouse neuroblastoma cells in vitro. C1 NIAID,ROCKY MT LABS,PERSISTENT VIRAL DIS LAB,HAMILTON,MT 59840. NR 29 TC 22 Z9 22 U1 0 U2 1 PU ACADEMIC PRESS (LONDON) LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0882-4010 J9 MICROB PATHOGENESIS JI Microb. Pathog. PD JAN PY 1995 VL 18 IS 1 BP 1 EP 9 PG 9 WC Immunology; Microbiology SC Immunology; Microbiology GA QM056 UT WOS:A1995QM05600001 PM 7783594 ER PT J AU CANCILLA, MR DAVIDSON, BE HILLIER, AJ NGUYEN, NY THOMPSON, J AF CANCILLA, MR DAVIDSON, BE HILLIER, AJ NGUYEN, NY THOMPSON, J TI THE LACTOCOCCUS-LACTIS TRIOSEPHOSPHATE ISOMERASE GENE, TPI, IS MONOCISTRONIC SO MICROBIOLOGY-UK LA English DT Article DE LACTOCOCCUS LACTIS; TRIOSEPHOSPHATE ISOMERASE; TRANSCRIPT ANALYSIS; BIASED CODON USAGE; ENZYME PURIFICATION ID TAGATOSE 6-PHOSPHATE PATHWAY; NUCLEOTIDE-SEQUENCE; STREPTOCOCCUS-LACTIS; ESCHERICHIA-COLI; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; BACILLUS-STEAROTHERMOPHILUS; CORYNEBACTERIUM-GLUTAMICUM; PHOSPHOTRANSFERASE SYSTEM; TRANSCRIPTIONAL ANALYSIS; MOLECULAR-CLONING AB Triosephosphate isomerase (EC 5.3.1.1) from Lactococcus lactis was purified to electrophoretic homogeneity. Approximately 3 mg purified enzyme (specific activity 3300 U mg(-1)) was obtained from 70 g (wet wt) cells. In solution, triosephosphate isomerase (pI 4.0-4.4) was observed to exist as a homodimer (M(r) 57000) of noncovalently linked subunits. The sequence of the first 37 amino acid residues from the NH2-terminus were determined by step-wise Edman degradation. This sequence, and that of a region conserved in all known bacterial triosephosphate isomerases, was used to design oligonucleotide primers for the synthesis of a lactococcal tpi probe by PCR. The probe was used to isolate a molecular clone of tpi from a lambda GEM11 library of L. lactic LM0230 DNA. The nucleotide sequence of tpi predicted a protein of 252 amino acids with the same NH2-terminal sequence as that determined for the purified enzyme and a subunit M(r) of 26802 after removal of the NH2-terminal methionine. Escherichia coli cells harbouring a plasmid containing tpi had 15-fold higher triosephosphate isomerase activity than isogenic plasmid-free cells, confirming the identity of the cloned gene. Northern analysis of L. lactis LM0230 RNA showed that a 900 base transcript hybridized with tpi. The 5' end of the transcript was determined by primer extension analysis to be a G located 64 bp upstream from the tpi start codon. These transcript analyses indicated that in L. lactis, tpi is expressed on a monocistronic transcript. Nucleotide sequencing indicated that the DNA adjacent to tpi did not encode another Embden-Meyerhoff-Parnas pathway enzyme. The location of tpi on the L. lactis DL11 chromosome map was determined to be between map coordinates 1.818 and 1.978. C1 UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM,PARKVILLE,VIC 3052,AUSTRALIA. CSIRO,DIV FOOD SCI & TECHNOL,DAIRY RES LAB,HIGHETT,VIC 3190,AUSTRALIA. US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,BETHESDA,MD 20892. NIDR,MICROBIAL ECOL LAB,BETHESDA,MD 20892. NR 49 TC 15 Z9 16 U1 0 U2 1 PU SOC GENERAL MICROBIOLOGY PI READING PA HARVEST HOUSE 62 LONDON ROAD, READING, BERKS, ENGLAND RG1 5AS SN 1350-0872 J9 MICROBIOL-UK JI Microbiol.-UK PD JAN PY 1995 VL 141 BP 229 EP 238 PN 1 PG 10 WC Microbiology SC Microbiology GA QC616 UT WOS:A1995QC61600028 PM 7534588 ER PT S AU Smith, JC AF Smith, JC BE Semple, SJG Adams, L Whipp, BJ TI New computational models of the respiratory oscillator in mammals SO MODELING AND CONTROL OF VENTILATION SE Advances in Experimental Medicine and Biology LA English DT Proceedings Paper CT London Conference on Modeling and Control of Ventilation (LCMCV) CY SEP 17-20, 1994 CL EGHAM, ENGLAND ID RHYTHM; MECHANISMS; NEURONS RP Smith, JC (reprint author), NINCDS, NIH, NEURAL CONTROL LAB, BETHESDA, MD 20892 USA. NR 10 TC 7 Z9 7 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45180-8 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 393 BP 7 EP 13 PG 7 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA BE85H UT WOS:A1995BE85H00002 PM 8629522 ER PT B AU JERNIGAN, RL YOUNG, L COVELL, DG MIYAZAWA, S AF JERNIGAN, RL YOUNG, L COVELL, DG MIYAZAWA, S BE Pullman, A Jortner, J Pullman, B TI Applications of empirical amino acid potential functions SO MODELLING OF BIOMOLECULAR STRUCTURES AND MECHANISMS SE JERUSALEM SYMPOSIA ON QUANTUM CHEMISTRY AND BIOCHEMISTRY LA English DT Proceedings Paper CT 27th Jerusalem Symposium on Quantum Chemistry and Biochemistry - Modelling of Biomolecular Structures and Mechanisms CY MAY 23-26, 1994 CL JERUSALEM, ISRAEL SP Israel Acad Sci & Humanities, Hebrew Univ Jerusalem, Biol Physico Chim Paris, Fdn Edmond Rothschild DE PROTEIN BINDING; EMPIRICAL POTENTIAL ENERGIES; AMINO ACID SUBSTITUTIONS; PROTEIN FOLD ASSESSMENT; HYDROPHOBICITY C1 NCI,MATH BIOL LAB,MOLEC STRUCT SECT,BETHESDA,MD 20892. RI Jernigan, Robert/A-5421-2012 NR 0 TC 1 Z9 1 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS BN 0-7923-3102-8 J9 JERUS SYM Q PY 1995 VL 27 BP 151 EP 166 PG 16 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA BD26J UT WOS:A1995BD26J00013 ER PT B AU ZHOU, HX AF ZHOU, HX BE Pullman, A Jortner, J Pullman, B TI Continuum-model studies of redox reactions, complex formation, and electron transfer: The paradigm of cytochrome c and cytochrome c peroxidase SO MODELLING OF BIOMOLECULAR STRUCTURES AND MECHANISMS SE JERUSALEM SYMPOSIA ON QUANTUM CHEMISTRY AND BIOCHEMISTRY LA English DT Proceedings Paper CT 27th Jerusalem Symposium on Quantum Chemistry and Biochemistry - Modelling of Biomolecular Structures and Mechanisms CY MAY 23-26, 1994 CL JERUSALEM, ISRAEL SP Israel Acad Sci & Humanities, Hebrew Univ Jerusalem, Biol Physico Chim Paris, Fdn Edmond Rothschild DE OXIDATION-REDUCTION; COMPLEX FORMATION; ELECTRON TRANSFER; CYTOCHROME C; CYTOCHROME C PEROXIDASE; ELECTROSTATIC INTERACTIONS; DIELECTRIC CONTINUUM MODEL C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS BN 0-7923-3102-8 J9 JERUS SYM Q PY 1995 VL 27 BP 381 EP 398 PG 18 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA BD26J UT WOS:A1995BD26J00030 ER PT S AU GOUPY, J MENET, JM SHINOMIYA, K ITO, Y AF GOUPY, J MENET, JM SHINOMIYA, K ITO, Y BE Conway, WD Petroski, RJ TI CROSS-AXIS COIL PLANET CENTRIFUGE - USE OF EXPERIMENTAL-DESIGN TO DETERMINE OPTIMAL SETTINGS SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, ID PERFORMING COUNTERCURRENT CHROMATOGRAPHY; STATIONARY-PHASE RETENTION; HYDRODYNAMIC MECHANISM; APPARATUS; PROTEINS; SYSTEMS; XLL AB It has proved difficult to optimize the sixth cross-axis Coil Planet Centrifuge designed by Dr. Ito for retention of stationary phase. Graphical analysis of the trial data did not give precise indications of the optimal settings. We have therefore applied the method of experimental designs to the same experimental data obtained from the sixth cross-axis prototype. This method allows the calculation of the effect of each studied parameter along with their multiple interactions. The effects of the interactions of the four solvent systems studied were found to be greater than those of the main individual factors. Experimental designs method provided more precise optimal settings than the original graphical method and allowed us to calculate the interaction effects. C1 NIHON UNIV,COLL PHARM,FUNABASHI,CHIBA 274,JAPAN. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP GOUPY, J (reprint author), ECOLE SUPER PHYS & CHIM IND VILLE PARIS,CHIM ANALYT LAB,CNRS,URA 437,10 RUE VAUQUELIN,F-75231 PARIS 05,FRANCE. NR 7 TC 1 Z9 1 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 47 EP 61 PG 15 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00004 ER PT S AU CAI, DG GU, MJ ZHU, GP ZHANG, JD JIN, T ZHANG, TY ITO, Y AF CAI, DG GU, MJ ZHU, GP ZHANG, JD JIN, T ZHANG, TY ITO, Y BE Conway, WD Petroski, RJ TI SEPARATION OF 3 ALKALOIDS FROM SENECIO-FUBERI HEMSL BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, AB Three alkaloids, squalidine, platyphylline, and neoplatyphylline from Senecio fuberi Hems1, were completely separated by high-speed counter-current chromatography (CCC). Separation was performed with a two-phase solvent system composed of chloroform/0.07M sodium phosphate-0.04M citrate buffer (pH 6.20-6.45) (1:1) in which the upper aqueous phase served as the stationary phase, and the lower chloroform phase as the mobile phase. Each alkaloid was identified by the physical and chemical constants as well as TLC and mass spectral data. Platyphylline and neoplatyphylline are cis-trans isomers which are difficult to separate by other means on a preparative scale. C1 BEIJING INST NEW TECHNOL APPLICAT,BEIJING 100035,PEOPLES R CHINA. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP CAI, DG (reprint author), PEOPLES LIBERAT ARMYS NAVY,PHARM RES CTR,SHANGHAI 200083,PEOPLES R CHINA. NR 7 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 87 EP 91 PG 5 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00007 ER PT S AU OKA, H IKAI, Y YAMADA, S HAYAKAWA, J HARADA, KI SUZUKI, M NAKAZAWA, H ITO, Y AF OKA, H IKAI, Y YAMADA, S HAYAKAWA, J HARADA, KI SUZUKI, M NAKAZAWA, H ITO, Y BE Conway, WD Petroski, RJ TI SEPARATION OF GARDENIA YELLOW COMPONENTS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, ID JASMINOIDES ELLIS; COLOR AB High-speed countercurrent chromatography (HSCCC) has been successfully applied to preparative separation of gardenia yellow components. Three components, geniposide, trans-crocin, and 13-cis-crocin, were isolated from 25mg of the sample using a two-phase solvent system composed of ethyl acetate-n-butanol-water (2:3:5). Due to the exposure to uv light emitted from the fluorescent room lamp, trans-crocin and 13-cis-crocin were photoisomerized to 13-cis- and trans-forms, respectively. It is also the first time that the presence of 13-cis-crocin and two glycosyl esters of crocetin in gardenia yellow is demonstrated by frit fast atom bombardment liquid chromatography/mass spectrometry and HPLC with photodiode array detection. C1 MEIJO UNIV,FAC PHARM,TENPAKU KU,NAGOYA,AICHI 468,JAPAN. NATL INST PUBL HLTH,MINATO KU,TOKYO 108,JAPAN. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP OKA, H (reprint author), AICHI PREFECTURAL INST PUBL HLTH,KITA KU,7-6 TSUJI MACHI,NAGOYA,AICHI 462,JAPAN. NR 15 TC 10 Z9 10 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 92 EP 106 PG 15 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00008 ER PT S AU DU, QZ XIONG, XP ITO, Y AF DU, QZ XIONG, XP ITO, Y BE Conway, WD Petroski, RJ TI SEPARATION OF CUCURBITACIN-B AND CUCURBITACIN-E FROM FRUIT BASE OF CUCUMIS-MELO L BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, AB Cucurbitacin B (CuB) and cucurbitacin E (CuE) were separated from the fruit base of Cucumis melo L. by high-speed countercurrent chromatography (CCC) using a two-phase solvent system composed of hexane/ethyl acetate/methanol/water (12:24:16:9 by volume). Three successive 300mg injections of sample, indicated by HPLC assay to contain 61.4% CuB and 35.1% CuE, were made at leap-frog intervals, prior to emergence of the second peak, thereby greatly reducing the overall separation time. Measurement of the stationary phase retention indicated that many more sample injections can be made without renewing the column contents. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP DU, QZ (reprint author), CHINESE ACAD AGR SCI,TEA RES INST,HANGZHOU 310008,PEOPLES R CHINA. NR 5 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 107 EP 110 PG 4 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00009 ER PT S AU KNIGHT, M ITO, Y CHANDRASEKHAR, B TAKAHASHI, K MUKHERJEE, AB AF KNIGHT, M ITO, Y CHANDRASEKHAR, B TAKAHASHI, K MUKHERJEE, AB BE Conway, WD Petroski, RJ TI PURIFICATION OF THE HUMAN IMMUNODEFICIENCY VIRUS-1 ASPARTYL PROTEINASE SUBSTRATE PEPTIDE SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, ID SYNTHETIC HIV-1 PROTEASE; RETROVIRAL PROTEASES; RESOLUTION; SEQUENCE; GAG AB A rapid and sensitive assay for the aspartyl protease enzyme of the HIV-1 virus is being developed for detecting potential inhibitors. A tritiated peptide serves as the substrate and after the reaction, the radiolabeled product is counted in solution while the substrate is removed from solution by adsorption onto charcoal added to the tube as a tablet. The labeled substrate was synthesized with an unsaturated proline in the sequence. After tritiation the radiolabeled substrate peptide was isolated by rapid countercurrent chromatography. The tritiation reaction was loaded on a multi-layer coil planet centrifuge in the solvent system t-butyl methyl ether, acetonitrile and 1% aq. trifluoroacetic acid (2:2:3 by volume) and chromatographed at a now of 120 ml/hr and a centrifugation rate of 800 rpm. The excess radioactive side products were eluted after the solvent front followed by the peptide. The peptide was separated within 1 hr and could be directly used in the experiments. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. NICHHD,HUMAN GENET BRANCH,BETHESDA,MD 20892. RP KNIGHT, M (reprint author), PEPTIDE TECHNOL CORP,8401 HELGERMAN COURT,GAITHERSBURG,MD 20877, USA. NR 19 TC 1 Z9 1 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 111 EP 118 PG 8 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00010 ER PT S AU SHIBUSAWA, Y CHIBA, T MATSUMOTO, U ITO, Y AF SHIBUSAWA, Y CHIBA, T MATSUMOTO, U ITO, Y BE Conway, WD Petroski, RJ TI COUNTERCURRENT CHROMATOGRAPHIC ISOLATION OF HIGH-DENSITY-LIPOPROTEIN AND LOW-DENSITY-LIPOPROTEIN FRACTIONS FROM HUMAN SERUM SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, ID GEL-PERMEATION CHROMATOGRAPHY; COIL PLANET CENTRIFUGE; CHOLESTEROL; SEPARATION AB High- and low-density lipoproteins (HDLs, LDLs) were fractionated by countercurrent chromatography using the type-XL cross-axis coil planet centrifuge using two-phase aqueous polymer systems. The best separations were achieved with a polymer phase system composed of 16%(w/w) polyethylene glycol 1000 and 12.5%(w/w) dibasic potassium phosphate at pH 9.4 by eluting the lower phase at 2.0 ml/min. HDL-LDL fractions containing 1.26, 1.27 and 0.42 mg/ml of phospholipids, cholesterols and triglycerides, respectively, were collected from 4 ml of human serum within 3 h. The presence of HDLs and LDLs were confirmed by gel electrophoresis with Sudan Black B staining. The HDL-LDL fractions were found to be free of serum proteins in SDS polyacrylamide slab gel electrophoresis. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP SHIBUSAWA, Y (reprint author), TOKYO COLL PHARM,DIV ANALYT CHEM,1432-1 HORINOUCHI,HACHIOJI,TOKYO 19203,JAPAN. NR 15 TC 1 Z9 1 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 119 EP 128 PG 10 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00011 ER PT S AU ITO, Y SHINOMIYA, K FALES, HM WEISZ, A SCHER, AL AF ITO, Y SHINOMIYA, K FALES, HM WEISZ, A SCHER, AL BE Conway, WD Petroski, RJ TI PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY - A NEW TECHNIQUE FOR PREPARATIVE SEPARATION SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, AB pH-Zone-refining countercurrent chromatography (CCC) separates organic acids and bases into a succession of highly concentrated rectangular peaks that elute according to their pK(a)s and hydrophobicities. The hydrodynamic mechanism of the present method is illustrated along with a simple mathematical analysis. Examples of the application of the present method are described to demonstrate its advantage over conventional CCC. Similarities and differences between pH-zone-refining CCC, displacement chromatography, and isotachophoresis are discussed. C1 US FDA,CTR FOOD SAFETY & APPL NUTR,OFF COSMET & COLORS,WASHINGTON,DC 20204. RP ITO, Y (reprint author), NHLBI,BIOPHYS CHEM LAB,BLDG 10,ROOM 7N322,BETHESDA,MD 20892, USA. NR 11 TC 12 Z9 13 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 156 EP 183 PG 28 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00014 ER PT S AU SCHER, AL ITO, Y AF SCHER, AL ITO, Y BE Conway, WD Petroski, RJ TI EQUILIBRIUM-MODEL FOR PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, AB Theoretical chromatograms for preparative separations of organic acids by pH-zone-refining countercurrent chromatography (CCC) were simulated using a computer program. Countercurrent distribution was used as a model. The simulated chromatograms contain flat-top peaks with steep sides and are consistent with experimental chromatograms in which organic acids in an organic stationary phase are separated by elution with aqueous base. The effects of the pK(a)s, partition ratios (K(D)s), concentrations of the sample acids, and concentrations of the retaining acid and eluting base were investigated. Separations depended on differences in the pK(a)s and K(D)s of the acids, Mixtures of acids with Delta p(K-d/K-D) = 1 were theoretically separated with recoveries of 99% of each acid with greater than or equal to 99% purity, even on a low-efficiency column (275 theoretical plates). These simulations are valuable for predicting and optimizing pH-zone-refining CCC separations. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP SCHER, AL (reprint author), US FDA,CTR FOOD SAFETY & APPL NUTR,OFF COSMET & COLORS,WASHINGTON,DC 20204, USA. NR 7 TC 2 Z9 2 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 184 EP 202 PG 19 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00015 ER PT S AU WEISZ, A ANDRZEJEWSKI, D SHINOMIYA, K ITO, Y AF WEISZ, A ANDRZEJEWSKI, D SHINOMIYA, K ITO, Y BE Conway, WD Petroski, RJ TI PREPARATIVE SEPARATION OF COMPONENTS OF COMMERCIAL 4,5,6,7-TETRACHLOROFLUORESCEIN BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, ID DYES; STANDARDIZATION; PURIFICATION; STAINS AB Samples of commercial 4,5,6,7-tetrachlorofluorescein (TCF) were subjected to pH-zone-refining countercurrent chromatography (pH-zone-refining CCC) to separate the main component and its contaminants. The study involved sample portions of 350 mg, 1 g, and 2 g, each with similar to 91% TCF, and a 5-g sample portion containing similar to 64.7% TCF. The separations were achieved with commercially available CCC centrifuges. The two-phase solvent systems used were diethyl ether-acetonitrile-10 mM aqueous ammonium acetate (4:1:5) and methyl tert-butyl ether-acetonitrile-water (4:1:5). Trifluoroacetic acid was added to either the sample solution or the (upper) stationary phase to serve as the retainer acid. Aqueous ammonia was added to the (lower) mobile phase to serve as the eluent base. The TCF separations required relatively short times (e.g., 5 h for the 5-g separation). The yield of the recovered TCF varied between similar to 65% for the 350-mg separation and 78.2 to similar to 89% for the gram-quantity separations. In all the separations, the recovered TCF had greater than 99.5% purity. Tetrachlorophthalic acid, 2,3,4-trichloro-6-hydroxyxanthone-1 -carboxylic acid, 2-(2',4' -dihydroxybenzoyl)tetrachlorobenzoic acid, and a new compound whose proposed structure is 10,11,12-trichloro-3H-[1]benzopyrano[2,3,4-k,1]xanthen-3-one were contaminants isolated from the 350-mg separation. A brief account of how each contaminant might be formed is also presented. C1 US FDA,OFF SCI ANAL & SUPPORT,WASHINGTON,DC 20204. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP WEISZ, A (reprint author), US FDA,OFF COSMET & COLORS,WASHINGTON,DC 20204, USA. NR 25 TC 5 Z9 5 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 203 EP 217 PG 15 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00016 ER PT S AU SHINOMIYA, K WEISZ, A ITO, Y AF SHINOMIYA, K WEISZ, A ITO, Y BE Conway, WD Petroski, RJ TI PURIFICATION OF 4,5,6,7-TETRACHLOROFLUORESCEIN BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY - EFFECTS OF SAMPLE-SIZE, CONCENTRATION OF ELUENT BASE, AND CHOICE OF RETAINER ACID SO MODERN COUNTERCURRENT CHROMATOGRAPHY SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Modern Countercurrent Chromatography, at the 206th National Meeting of the American-Chemical-Society CY AUG 22-27, 1993 CL CHICAGO, IL SP Amer Chem Soc, Div Agr & Food Cje, AB Various factors involved in pH-zone-refining countercurrent chromatography were investigated in the purification of 4,5,6,7-tetrachlorofluorescein, using a two-phase solvent system composed of diethyl ether-acetonitrile-10 mM ammonium acetate (4:1:5). Trifluoroacetic acid (TFA) was added to the sample solution as a retainer acid. The results indicated that 1) increasing the amount of dye improved the yield of pare compounds; 2) increasing the concentration of ammonia in the solvent system increased the concentration of analytes in the mobile phase and shortened the retention time of the major peak; and 3) ammonium acetate in the solvent system could be eliminated and TFA in the sample solution could be replaced by acetic acid without significantly affecting the purification. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. US FDA,CTR FOOD SAFETY & APPL NUTR,OFF COSMET & COLORS,WASHINGTON,DC 20204. NR 5 TC 1 Z9 1 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3167-2 J9 ACS SYM SER PY 1995 VL 593 BP 218 EP 230 PG 13 WC Chemistry, Analytical SC Chemistry GA BD63U UT WOS:A1995BD63U00017 ER PT S AU EICHACKER, PQ NATANSON, C AF EICHACKER, PQ NATANSON, C BE Tellado, JM Forse, RA Solomkin, JS TI Neutrophil-directed therapies in sepsis: Studies in a canine model SO MODULATION OF THE INFLAMMATORY RESPONSE IN SEVERE SEPSIS SE PROGRESS IN SURGERY LA English DT Proceedings Paper CT International Symposium on Modulation of the Inflammatory Response in Severe Sepsis CY SEP 16-17, 1993 CL MADRID, SPAIN RP NIH,CTR CLIN,DEPT CRIT CARE MED,BLDG 10,ROOM 7D43,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0079-6824 BN 3-8055-6041-9 J9 PROG SURG JI Prog.Surg. PY 1995 VL 20 BP 67 EP 76 PG 10 WC Immunology; Surgery SC Immunology; Surgery GA BD68H UT WOS:A1995BD68H00007 ER PT J AU VAIDYA, AB MURATOVA, O GUINET, F KEISTER, D WELLEMS, TE KASLOW, DC AF VAIDYA, AB MURATOVA, O GUINET, F KEISTER, D WELLEMS, TE KASLOW, DC TI A GENETIC-LOCUS ON PLASMODIUM-FALCIPARUM CHROMOSOME-12 LINKED TO A DEFECT IN MOSQUITO-INFECTIVITY AND MALE GAMETOGENESIS SO MOLECULAR AND BIOCHEMICAL PARASITOLOGY LA English DT Article DE GENETICS; PLASMODIUM FALCIPARUM; EXFLAGELLATION; GAMETOGENESIS ID CHLOROQUINE-RESISTANCE; EXFLAGELLATION; INHERITANCE; CROSS AB Infection of mosquitoes by Plasmodium spp. requires sexual differentiation of the malarial parasite in the vertebrate host and mating of the heterogametes in the vector midget. A Plasmodium falciparum clone, Dd2, differentiates into normal-appearing gametocytes, yet poorly infects mosquitoes. The Dd2 clone, however, effectively cross-fertilized HB3, a Central American P. falciparum clone, and yielded several independent recombinant progeny. We have examined 11 HB2 X Dd2 progeny for their ability to infect mosquitoes and to differentiate into male gametes. Our analyses indicate that the poor mosquito-infectivity of the Dd2 clone results from a defect in male gametogenesis. This defect was inherited as a single locus in the independent recombinant progeny of HB3 X Dd2. Comparison with a restriction fragment length polymorphism map of the HB2 X Dd2 cross indicates that the defective phenotype of Dd2 maps to a locus on P. falciparum chromosome 12. This genetic locus may contain determinants that play a crucial role in male gametogenesis by P. falciparum. C1 NIAID,MALARIA RES LAB,BETHESDA,MD. RP VAIDYA, AB (reprint author), HAHNEMANN UNIV,DEPT MICROBIOL & IMMUNOL,MAIL STOP 405,BROAD & VINE ST,PHILADELPHIA,PA 19102, USA. FU NIAID NIH HHS [AI28398, R01 AI028398] NR 20 TC 40 Z9 40 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-6851 J9 MOL BIOCHEM PARASIT JI Mol. Biochem. Parasitol. PD JAN PY 1995 VL 69 IS 1 BP 65 EP 71 DI 10.1016/0166-6851(94)00199-W PG 7 WC Biochemistry & Molecular Biology; Parasitology SC Biochemistry & Molecular Biology; Parasitology GA QE150 UT WOS:A1995QE15000007 PM 7723789 ER PT J AU KOCKEN, CHM MILEK, RLB LENSEN, THW KASLOW, DC SCHOENMAKERS, JGG KONINGS, RNH AF KOCKEN, CHM MILEK, RLB LENSEN, THW KASLOW, DC SCHOENMAKERS, JGG KONINGS, RNH TI MINIMAL VARIATION IN THE TRANSMISSION-BLOCKING VACCINE CANDIDATE PFS48/45 OF THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM SO MOLECULAR AND BIOCHEMICAL PARASITOLOGY LA English DT Note DE PLASMODIUM FALCIPARUM; PFS48/45; MALARIA VACCINE CANDIDATE; TRANSMISSION BLOCKING ID MONOCLONAL-ANTIBODIES; TARGET ANTIGENS; PCR-SSCP; EXPRESSION; EPITOPES C1 UNIV NIJMEGEN,FAC MED,INST MED PARASITOL,6525 GA NIJMEGEN,NETHERLANDS. UNIV NIJMEGEN,FAC SCI,DEPT MOLEC BIOL,6525 ED NIJMEGEN,NETHERLANDS. NIAID,PARASIT DIS LAB,BETHESDA,MD 20892. NR 17 TC 24 Z9 24 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-6851 J9 MOL BIOCHEM PARASIT JI Mol. Biochem. Parasitol. PD JAN PY 1995 VL 69 IS 1 BP 115 EP 118 DI 10.1016/0166-6851(94)00193-Q PG 4 WC Biochemistry & Molecular Biology; Parasitology SC Biochemistry & Molecular Biology; Parasitology GA QE150 UT WOS:A1995QE15000013 PM 7723779 ER PT J AU MYMRYK, JS BERARD, D HAGER, GL ARCHER, TK AF MYMRYK, JS BERARD, D HAGER, GL ARCHER, TK TI MOUSE MAMMARY-TUMOR VIRUS CHROMATIN IN HUMAN BREAST-CANCER CELLS IS CONSTITUTIVELY HYPERSENSITIVE AND EXHIBITS STEROID HORMONE-INDEPENDENT LOADING OF TRANSCRIPTION FACTORS IN-VIVO SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID GLUCOCORTICOID RESPONSE ELEMENT; PROGESTERONE-RECEPTOR; CHLORAMPHENICOL ACETYLTRANSFERASE; NUCLEAR-LOCALIZATION; NONRECEPTOR FACTORS; NEGATIVE REGULATION; GENE ACTIVATION; MMTV PROMOTER; MESSENGER-RNA; INDUCTION AB We have stably introduced a reporter gene under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) into human T47D breast cancer cells to study the action of the progesterone receptor (PR) on transcription from a chromatin template. Unexpectedly, the chromatin organization of the MMTV LTR in these human breast cancer cells differed markedly from what we have observed previously. The region adjacent to the transcription start site (-221 to -75) was found to be constitutively hypersensitive to restriction enzyme cleavage in the absence of hormone. This region is normally encompassed within the second nucleosome of a phased array of six nucleosomes that is assembled when the MMTV LTR is stably maintained in mouse cells. Characteristically, in these rodent cells, the identical DNA sequences show increased restriction enzyme cleavage only in the presence of glucocorticoid. The increased access of restriction enzymes observed in the human PR(+) cells was not observed in adjacent nucleosomes and was unaffected by treatment with the progesterone antagonist RU486. In addition, exonuclease III-dependent stops corresponding to the binding sites for nuclear factor 1 and the PR were observed before and after hormone treatment. These results indicate that MMTV chromatin replicated in these cells is organized into a constitutively open architecture and that this open chromatin state is accompanied by hormone-independent loading of a transcription factor complex that is normally excluded from uninduced chromatin. C1 UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL,LONDON,ON N6A 4L6,CANADA. UNIV WESTERN ONTARIO,DEPT BIOCHEM,LONDON,ON N6A 4L6,CANADA. UNIV WESTERN ONTARIO,DEPT ONCOL,LONDON,ON N6A 4L6,CANADA. NCI,MOLEC VIROL LAB,BETHESDA,MD 20892. NR 50 TC 40 Z9 41 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 26 EP 34 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300004 PM 7799933 ER PT J AU CHU, E TAKECHI, T JONES, KL VOELLER, DM COPUR, SM MALEY, GF MALEY, F SEGAL, S ALLEGRA, CJ AF CHU, E TAKECHI, T JONES, KL VOELLER, DM COPUR, SM MALEY, GF MALEY, F SEGAL, S ALLEGRA, CJ TI THYMIDYLATE SYNTHASE BINDS TO C-MYC RNA IN HUMAN COLON-CANCER CELLS AND IN-VITRO SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID 3' UNTRANSLATED REGION; MESSENGER-RNA; MOUSE FIBROBLASTS; ESCHERICHIA-COLI; PROTEIN BINDS; TRANSLATION; SYNTHETASE; FERRITIN; ENZYME; BACTERIOPHAGE-T4 AB Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS protein and the mRNA of the nuclear oncogene c-myc. TS protein is complexed in intact cells with the C-terminal coding region of c-myc mRNA that includes nucleotide positions 1625 to 1790. RNA electrophoretic gel mobility shift assays confirm a specific interaction between TS protein and c-myc mRNA and provide additional evidence that the C-terminal coding region represents an important cis-acting regulatory element. Further evidence demonstrates that the in vitro translational efficiency of c-myc mRNA is inhibited as a result of its direct interaction with TS protein. In addition, the presence of exogenous c-myc mRNA specifically relieves the inhibitory effects of TS protein on TS mRNA translation. C1 USN,NCI,MED ONCOL BRANCH,BETHESDA,MD 20889. NEW YORK STATE DEPT HLTH,WADSWORTH CTR LABS & RES,ALBANY,NY 12201. FU NCI NIH HHS [CA 44355] NR 35 TC 72 Z9 73 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 179 EP 185 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300021 PM 7799924 ER PT J AU LENG, XH BLANCO, J TSAI, SY OZATO, K OMALLEY, BW TSAI, MJ AF LENG, XH BLANCO, J TSAI, SY OZATO, K OMALLEY, BW TSAI, MJ TI MOUSE RETINOID-X RECEPTOR CONTAINS A SEPARABLE LIGAND-BINDING AND TRANSACTIVATION DOMAIN IN ITS E-REGION SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID THYROID-HORMONE RECEPTOR; TRANSCRIPTION FACTOR; RESPONSE ELEMENT; V-ERBA; GLUCOCORTICOID RECEPTOR; CONFORMATIONAL-CHANGES; SIGNALING PATHWAYS; NUCLEAR RECEPTORS; ONCOGENE PRODUCT; GENE-REGULATION AB Steroid, thyroid, and retinoid hormones exert their biological functions by interacting with their cognate nuclear receptors. Upon binding receptors, hormones induce a protease-resistant structural change in the receptor ligand-binding domain and subsequently activate the receptors. Utilizing partial proteolysis, we have been able to delineate a region in the mouse retinoid X receptor beta (mRXR beta) required for ligand binding. A separable activation domain within the mRXR beta E region has been identified. The activation domain, which is 21 amino acids in length, is located at the extreme C terminus of mRXR beta. This domain is not required for ligand binding since removal of this sequence neither eliminates the ligand-induced, protease-resistant conformational change nor alters the ligand-enhanced DNA binding. Furthermore, deletion of this activation domain converts the receptor into a transcriptional silencer. Finally, a further truncation of 9 amino acids (for a total of 30 amino acids) from the C terminus results in a mutant which does not undergo the protease-resistant conformational change and cannot bind DNA as a homodimer. Nevertheless, this mutant is still able to form a heterodimer with the thyroid hormone receptor. Therefore, homodimerization and heterodimerization can be distinguished by this nine-amino-acid sequence. C1 BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030. NICHHD,MOLEC GROWTH REGULAT LAB,BETHESDA,MD 20892. NR 54 TC 92 Z9 92 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 255 EP 263 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300029 PM 7799932 ER PT J AU ROMANO, PR GREEN, SR BARBER, GN MATHEWS, MB HINNEBUSCH, AG AF ROMANO, PR GREEN, SR BARBER, GN MATHEWS, MB HINNEBUSCH, AG TI STRUCTURAL REQUIREMENTS FOR DOUBLE-STRANDED-RNA BINDING, DIMERIZATION, AND ACTIVATION OF THE HUMAN EIF-2-ALPHA KINASE DAI IN SACCHAROMYCES-CEREVISIAE SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID DEPENDENT PROTEIN-KINASE; P68 KINASE; INITIATION FACTOR-2-ALPHA; FUNCTIONAL EXPRESSION; TRANSLATIONAL CONTROL; INTERFERON ACTION; MECHANISM; DOMAIN; YEAST; GCN2 AB The protein kinase DAI is activated upon viral infection of mammalian cells and inhibits protein synthesis by phosphorylation of the alpha subunit of translation initiation factor 2 (eIF-2 alpha). DAI is activated in vitro by double-stranded RNAs (dsRNAs), and binding of dsRNA is dependent on two copies of a conserved sequence motif located N terminal to the kinase domain in DAI. High-level expression of DAI in Saccharomyces cerevisiae cells is lethal because of hyperphosphorylation of eIF-2 alpha; at lower levels, DAI can functionally replace the protein kinase GCN2 and stimulate translation of GCN4 mRNA. These two phenotypes were used to characterize structural requirements for DAI function in vivo, by examining the effects of amino acid substitutions at matching positions in the two dsRNA-binding motifs and of replacing one copy of the motif with the other. We found that both copies of the dsRNA-binding motif are required for high-level kinase function and that the N-terminal copy is more important than the C-terminal copy for activation of DAI in S. cerevisiae. On the basis of these findings,,ve conclude that the requirements for dsRNA binding in vitro and for activation of DAI kinase function in vivo closely coincide. Two mutant alleles containing deletions of the first or second binding motif functionally complemented when coexpressed in yeast cells, strongly suggesting that the active form of DAI is a dimer. In accord with this conclusion, overexpression of four catalytically inactive alleles containing different deletions in the protein kinase domain interfered with wild-type DAI produced in the same cells. Interestingly, three inactivating point mutations in the kinase domain were all recessive, suggesting that dominant interference involves the formation of defective heterodimers rather than sequestration of dsRNA activators by mutant enzymes. We suggest that large structural alterations in the kinase domain impair an interaction between the two protomers in a DAT, dimer that is necessary for activation by dsRNA or for catalysis of eIF-2 alpha phosphorylation. C1 NICHHD, MOLEC GENET LAB, MOLEC GENET LOWER EUKARYOTES LAB, BETHESDA, MD 20892 USA. COLD SPRING HARBOR LAB, COLD SPRING HARBOR, NY 11724 USA. RIBOGENE INC, HAYWARD, CA 94545 USA. UNIV WASHINGTON, SCH MED, DEPT MICROBIOL, SEATTLE, WA 98195 USA. NR 41 TC 120 Z9 120 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 EI 1098-5549 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 365 EP 378 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300042 PM 7799945 ER PT J AU PUMIGLIA, K CHOW, YH FABIAN, J MORRISON, D DECKER, S JOVE, R AF PUMIGLIA, K CHOW, YH FABIAN, J MORRISON, D DECKER, S JOVE, R TI RAF-1 N-TERMINAL SEQUENCES NECESSARY FOR RAS-RAF INTERACTION AND SIGNAL-TRANSDUCTION SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID ACTIVATED PROTEIN-KINASE; MAP KINASE; IN-VITRO; PATHWAY; EXPRESSION; ONCOGENE; PHOSPHORYLATION; C-RAF-1; BINDING; GROWTH AB Raf-l is a serine/threonine protein kinase that transduces signals from cell surface receptors to the nucleus. Interaction of Ras with a regulatory domain in the N-terminal half of Raf-l is postulated to regulate Raf-l protein kinase and signaling activities. To better understand molecular interactions of Ras with Raf-l and regulation of the Raf-l kinase, a panel of Raf-l N-terminal mutants expressed in the baculovirus-insect cell system was used for mapping the precise region necessary for Ras interaction in the context of full-length, functional Raf-l kinase. An 80-amino-acid sequence in Raf-l between positions 53 and 132 was found to confer the ability to bind Ras protein in vitro and in infected insect cells. Deletion of residues 53 to 132 abolished Raf-l kinase activation by Ras in insect cells, indicating that activation of the Raf-l kinase by Res requires the capacity to physically interact with Ras. By contrast, deletion of this pas-binding site did not diminish activation of Raf-l kinase by Src, implying that Src and Ras can activate Raf-l through independent mechanisms. Significantly, Raf-l mutants lacking the entire zinc finger motif or containing substitutions of two critical cysteine residues in the zinc finger retained the ability to bind Ras and to be activated by this interaction. Consistent with results obtained in the baculovirus-insect cell system, deletion of residues 53 to 132 but not mutations in the zinc finger motif abrogated the ability of kinase-inactive, dominant negative Raf-l to block Ras-mediated signaling in Xenopus oocytes. Together, these results provide evidence that the direct physical interaction of Ras with Raf-l amino acids 53 to 132 is required for activation of the Raf-l kinase and signaling activities by Ras but not by Src. Furthermore, the adjacent zinc finger motif in Raf-l is not essential either for interaction with Ras or for activation of the Raf-l kinase. C1 UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,ANN ARBOR,MI 48109. UNIV MICHIGAN,SCH MED,CTR COMPREHENS CANC,ANN ARBOR,MI 48109. WARNER LAMBERT PARKE DAVIS,PARKE DAVIS PHARMACEUT RES DIV,ANN ARBOR,MI 48106. NCI,FREDERICK CANC RES & DEV CTR,MOLEC MECH CARCINOGENESIS LAB,FREDERICK,MD 21702. OI Pumiglia, Kevin/0000-0003-4655-0334 FU NCI NIH HHS [CA55652] NR 50 TC 47 Z9 47 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 398 EP 406 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300045 PM 7799948 ER PT J AU MASON, MM LEE, E WESTPHAL, H REITMAN, M AF MASON, MM LEE, E WESTPHAL, H REITMAN, M TI EXPRESSION OF THE CHICKEN BETA-GLOBIN GENE-CLUSTER IN MICE - CORRECT DEVELOPMENTAL EXPRESSION AND DISTRIBUTED CONTROL SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID LOCUS-CONTROL REGION; ERYTHROID TRANSCRIPTION FACTOR; TRANSGENIC MICE; ACTIVATION REGION; HYPERSENSITIVE SITES; CHROMATIN; ENHANCER; SWITCH; DNA; EVOLUTION AB To investigate the regulation of gene clusters, we introduced the entire chicken beta-globin cluster into mice. This 35-kb region includes the four globin genes (rho-beta(H)-beta(A)-epsilon), the four upstream hypersensitive sites, and the intergenic beta(A)/epsilon enhancer. The chicken globins are not arranged in order of developmental expression, which is unlike the case for the human beta-globin cluster, in which gene order plays a role in the regulation of globin expression. Mice carrying the chicken cluster expressed the transgenes,vith the same developmental patterns as seen in the chicken. Therefore, stage-specific erythroid transcriptional milieus existed before the divergence of birds and mammals and have been conserved since then. Mice bearing the complete cluster except for a deletion removing the beta(A)/epsilon enhancer displayed markedly reduced expression of the beta(H), beta(A) and epsilon genes with efficient (but variable) rho expression. Mice carrying the four genes and beta(A)/epsilon enhancer but without the upstream hypersensitive sites showed reduced expression of rho, beta(H), and beta(A), with variable expression of epsilon. We conclude that (i) all of the genes (except possibly rho) are under the control of both the upstream hypersensitive sites and the enhancer, (ii) the influence of the control elements can extend beyond the nearest active gene, (iii) a single element (the enhancer) can influence more than one gene in a single developmental stage, (iv) the enhancer can work bidirectionally, and (v) neither the upstream sites (as a group) nor the enhancer showed developmental stage specificity. Thus, the regulation of this cluster is achieved by interaction of two distinct control regions with each of the globin genes. C1 NIDDKD,DIABET BRANCH,BETHESDA,MD 20892. NICHHD,GENET MOLEC LAB,BETHESDA,MD 20892. RI Reitman, Marc/B-4448-2013 OI Reitman, Marc/0000-0002-0426-9475 NR 48 TC 36 Z9 37 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 407 EP 414 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300046 PM 7799949 ER PT J AU LUEDERS, KK FEWELL, JW MOROZOV, VE KUFF, EL AF LUEDERS, KK FEWELL, JW MOROZOV, VE KUFF, EL TI SELECTIVE EXPRESSION OF INTRACISTERNAL A-PARTICLE GENES IN ESTABLISHED MOUSE PLASMACYTOMAS (VOL 13, PG 7443, 1993) SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Correction, Addition RP LUEDERS, KK (reprint author), NCI,BIOCHEM LAB,BLDG 37,ROOM 4C-03,BETHESDA,MD 20892, USA. NR 1 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1995 VL 15 IS 1 BP 590 EP 590 PG 1 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA PX953 UT WOS:A1995PX95300065 PM 7799968 ER PT J AU MEIERHEUSLER, SC ZHU, XG JUGEAUBRY, C PERNIN, A BURGER, AG CHENG, SY MEIER, CA AF MEIERHEUSLER, SC ZHU, XG JUGEAUBRY, C PERNIN, A BURGER, AG CHENG, SY MEIER, CA TI MODULATION OF THYROID-HORMONE ACTION BY MUTANT THYROID-HORMONE RECEPTORS, C-ERBA-ALPHA-2 AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR - EVIDENCE FOR DIFFERENT MECHANISMS OF INHIBITION SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article DE THYROID HORMONE RECEPTORS; C-ERBA-ALPHA-2; PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR; RETINOID X RECEPTOR; DOMINANT NEGATIVE ACTION; RESISTANCE TO THYROID HORMONE ID RETINOID-X-RECEPTOR; DOMINANT-NEGATIVE INHIBITION; GENERALIZED RESISTANCE; RESPONSE ELEMENT; ACID RECEPTORS; LIGAND-BINDING; BETA-GENE; TRANSCRIPTIONAL REGULATION; BIFUNCTIONAL ENZYME; AUXILIARY PROTEINS AB Thyroid hormone action is not only determined by hormone availability, but also by target organ sensitivity. A dominant negative interaction is known to occur between thyroid hormone receptors (TRs) and the non-ligand binding splicing variant c-erbA alpha 2 as well as mutant TR beta 1 from kindreds with resistance to thyroid hormone. We compared the inhibitory effect of naturally occurring mutant hTR beta 1, artificially created hTR alpha 1 mutants, c-erbA alpha 2 and the human peroxisome proliferator-activated receptor (hPPAR) on three prototypic T3-response elements (TREs), TRE-PAL, DR+4 and TRE-LAP. The inhibitory effect of mutant hTR alpha 1 and beta 1 occurred only on TRE-LAP and to a minor degree on DR+4 when equimolar ratios of mutant/wildtype receptor were present. In contrast, the c-erbA alpha 2 splicing variant and the hPPAR inhibited TR action on all three TREs. Gel mobility shift experiments in the presence of T3 showed increased binding of mutant hTR alpha 1 and beta 1 only to TRE-LAP compared to the binding of wildtype hTRs, thereby explaining their TRE-selective dominant negative potency. Contrarily, equal amounts of c-erbA alpha 2 or hPPAR protein did not bind to either of the three response elements even in the presence of RXR. Since the TR:RXR heterodimers were only partially displaced from DNA in the presence of excess amounts of c-erbA alpha 2, it is likely that the TRE-unspecific dominant negative action of c-erbA alpha 2 is due in part to competition for DNA-binding and for TR-auxiliary proteins. In contrast, equimolar amounts of hPPAR completely inhibited the DNA-binding of hTR beta 1:RXR heterodimers, but not of TR:TR homodimers, suggesting that hPPAR has a higher RXR-binding affinity and is therefore a potent competitor for intranuclear RXR. Since thyroid hormones and peroxisome proliferators regulate in part a similar subset of target genes involved in fatty acid metabolism, these results suggest the possibility of cross-talk among the thyroid hormone and peroxisome proliferator signalling pathways. In summary, the results suggest that thyroid hormone action can be modulated by at least three different mechanisms: (i) increased binding of mutant hTRs to specific TREs; (ii) efficient competition for limiting amounts of RXR through the preferential formation of hPPAR:RXR, rather than TR:RXR heterodimers; and (iii) competition for binding to DNA and to auxiliary proteins other than RXR in the case of c-erbA alpha 2. C1 UNIV HOSP GENEVA,DIV ENDOCRINOL,THYROID UNIT,CH-1211 GENEVA 14,SWITZERLAND. NCI,MOLEC BIOL LAB,BETHESDA,MD 20892. UNIV HOSP GENEVA,DEPT MED,CH-1211 GENEVA 14,SWITZERLAND. NR 60 TC 26 Z9 27 U1 0 U2 1 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD JAN PY 1995 VL 107 IS 1 BP 55 EP 66 DI 10.1016/0303-7207(94)03422-P PG 12 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA QE784 UT WOS:A1995QE78400006 PM 7796935 ER PT S AU ELANI, D JACOBSON, KA SHAINBERG, A AF ELANI, D JACOBSON, KA SHAINBERG, A BE Sideman, S Beyar, R TI Regulation of adenosine receptors in cultured heart cells SO MOLECULAR AND SUBCELLULAR CARDIOLOGY: EFFECTS OF STRUCTURE AND FUNCTION SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT 9th Goldberg Workshop on Molecular and Subcellular Cardiology - Effects on Structure and Function CY DEC 04-08, 1994 CL HAIFA, ISRAEL SP Technion Israel Inst Technol, Israel Cardiol Soc, Henry & Viola Goldberg, New York ID ADENYLATE CYCLASE SYSTEM; CHICK ATRIAL MYOCYTES; VENTRICULAR MYOCYTES; RADIOLIGAND BINDING; MYOCARDIAL-CELLS; MECHANISM; THEOPHYLLINE; MUSCLE; CONTRACTILITY; ISOPROTERENOL AB A(1) adenosine receptors were studied on heart cells grown in cultures by the radioligand binding technique. Treatments with agents that accelerated heart rate for 3-4 days, caused an increase in the level of adenosine receptors. Treatments that attenuated heart rate, reduced the level of the receptors. Thus, the cardiac cells respond to environmental conditions affecting heart contraction so as to restore the basal rate of heart activity. C1 NIDDK,BIOORGAN CHEM LAB,MOLEC RECOGNIT SECT,BETHESDA,MD 20892. RP BAR ILAN UNIV,DEPT LIFE SCI,OTTO MEYERHOFF DRUG RECEPTOR CTR,IL-52900 RAMAT GAN,ISRAEL. OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20, Z99 DK999999] NR 33 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-45123-9 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 382 BP 205 EP 215 PG 11 WC Biochemistry & Molecular Biology; Cardiac & Cardiovascular Systems; Engineering, Biomedical SC Biochemistry & Molecular Biology; Cardiovascular System & Cardiology; Engineering GA BE24M UT WOS:A1995BE24M00021 PM 8540397 ER PT B AU Stadtman, ER AF Stadtman, ER BE Esser, K Martin, GM TI The status of oxidatively modified proteins as a marker of aging SO MOLECULAR ASPECTS OF AGING SE LIFE SCIENCES RESEARCH REPORT LA English DT Proceedings Paper CT Dahlem Workshop on Molecular Aspects of Aging CY FEB 13-18, 1994 CL BERLIN, GERMANY SP Freie Univ Berlin, Senat Stadt Berlin C1 NHLBI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 22 Z9 22 U1 0 U2 1 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD BN 0-471-95689-9 J9 LIFE SCI R PY 1995 VL 56 BP 129 EP 143 PG 15 WC Biochemistry & Molecular Biology; Cell Biology; Geriatrics & Gerontology SC Biochemistry & Molecular Biology; Cell Biology; Geriatrics & Gerontology GA BE45K UT WOS:A1995BE45K00010 ER PT S AU Philpot, RM Overby, L AF Philpot, RM Overby, L BE Arinc, E Schenkman, JB Hodgson, E TI Problems associated with assessment of the contribution of individual forms of cytochrome P450 to the metabolism of xenobiotics SO MOLECULAR ASPECTS OF OXIDATIVE DRUG METABOLIZING ENZYMES: THEIR SIGNIFICANCE IN ENVIRONMENTAL TOXICOLOGY, CHEMICAL CARCINOGENESIS AND HEALTH SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES H, CELL BIOLOGY LA English DT Proceedings Paper CT NATO Advanced Study Institute on Molecular Aspects of Drug Metabolizing Enzymes CY JUN 20-JUL 02, 1993 CL KUSADASI, TURKEY SP NATO C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 1010-8793 BN 3-540-58856-6 J9 NATO ADV SCI INST SE PY 1995 VL 90 BP 193 EP 207 PG 15 WC Biochemistry & Molecular Biology; Cell Biology; Toxicology SC Biochemistry & Molecular Biology; Cell Biology; Toxicology GA BE33R UT WOS:A1995BE33R00010 ER PT S AU Philpot, RM AttaAsafoAdjei, E Nikbakht, K Burnett, V Lawton, MP AF Philpot, RM AttaAsafoAdjei, E Nikbakht, K Burnett, V Lawton, MP BE Arinc, E Schenkman, JB Hodgson, E TI Emergence of the flavin-containing monooxygenase gene family: Molecular biology, structure, and function SO MOLECULAR ASPECTS OF OXIDATIVE DRUG METABOLIZING ENZYMES: THEIR SIGNIFICANCE IN ENVIRONMENTAL TOXICOLOGY, CHEMICAL CARCINOGENESIS AND HEALTH SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES H, CELL BIOLOGY LA English DT Proceedings Paper CT NATO Advanced Study Institute on Molecular Aspects of Drug Metabolizing Enzymes CY JUN 20-JUL 02, 1993 CL KUSADASI, TURKEY SP NATO C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 1010-8793 BN 3-540-58856-6 J9 NATO ADV SCI INST SE PY 1995 VL 90 BP 209 EP 224 PG 16 WC Biochemistry & Molecular Biology; Cell Biology; Toxicology SC Biochemistry & Molecular Biology; Cell Biology; Toxicology GA BE33R UT WOS:A1995BE33R00011 ER PT J AU USDIN, K CHEVRET, P CATZEFLIS, FM VERONA, R FURANO, AV AF USDIN, K CHEVRET, P CATZEFLIS, FM VERONA, R FURANO, AV TI L1 (LINE-1) RETROTRANSPOSABLE ELEMENTS PROVIDE A FOSSIL RECORD OF THE PHYLOGENETIC HISTORY OF MURID RODENTS SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE L1/LINE; EVOLUTION; RODENT; TAXONOMY; PHYLOGENY; RETROTRANSPOSON; REPEATED DNA ID REPEAT FAMILY; MUS-DOMESTICUS; DNA FAMILY; EVOLUTION; SEQUENCE; MICE; DIVERGENCE; OTOMYINAE; MAMMALIA; TEMPO AB The single most difficult problem in phylogenetic analysis is deciding whether a shared taxonomic character is due to common ancestry or one that appeared independently due to convergence, parallelism, or reversion to an ancestral state. Mammalian LI retrotransposons undergo periodic amplifications in which multiple copies of the elements are interspersed in the genome. Because these elements apparently are transmitted only by inheritance and are retained in the genome, a shared L1 amplification event can only be an inherited ancestral character. We propose that L1 amplification events can be an excellent tool for analyzing mammalian evolution and demonstrate here how we addressed several refractory problems in rodent systematics using L1 DNA as a taxonomic character. C1 NIDKD,GENOM STRUCT & FUNCT SECT,BETHESDA,MD 20892. UNIV MONTPELLIER 2,INST SCI EVOLUT,MONTPELLIER,FRANCE. RI Chevret, Pascale/B-7299-2009 NR 47 TC 32 Z9 33 U1 0 U2 4 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0737-4038 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD JAN PY 1995 VL 12 IS 1 BP 73 EP 82 PG 10 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA QA174 UT WOS:A1995QA17400007 PM 7877498 ER PT J AU MATHIOPOULOS, KD POWELL, JD MCCUTCHAN, TF AF MATHIOPOULOS, KD POWELL, JD MCCUTCHAN, TF TI AN ANCHORED RESTRICTION-MAPPING APPROACH APPLIED TO THE GENETIC-ANALYSIS OF THE ANOPHELES-GAMBIAE MALARIA VECTOR COMPLEX-1 SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE MITOCHONDRIAL DNA; SINGLE-COPY NUCLEAR DNA; RIBOSOMAL DNA; MOLECULAR PHYLOGENIES; ANCHORED RESTRICTION MAPPING; ANOPHELES GAMBIAE COMPLEX ID DROSOPHILA; CLEAVAGE; TREES; FLOW AB We introduce here a simple approach for rapidly determining restriction maps for a number of regions of a genome; this involves ''anchoring'' a map with a rare restriction site (in this case the seldom-cutting EagI) followed by partial digestion of a frequent-cutting enzyme (e.g., Sau3A). We applied this technology to five species of the Anapheles gambiae complex. In a single Southern blot we obtained about a 15-kb restriction map each for the mtDNA, rRNA gene, and a scnDNA region for each of five species. Phylogenetic analyses of these regions yield trees at odds with the more traditional chromosome inversion-based trees. The value of the approach for systematic purposes is the ease with which several large, independent regions of the genome can be quickly assayed for molecular variation. C1 NIAID,MALARIA RES LAB,BETHESDA,MD 20892. YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06520. OI Mathiopoulos, Kostas/0000-0002-2875-5697 FU NIAID NIH HHS [AI35215] NR 23 TC 5 Z9 5 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0737-4038 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD JAN PY 1995 VL 12 IS 1 BP 103 EP 112 PG 10 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA QA174 UT WOS:A1995QA17400010 PM 7877485 ER PT J AU MEESE, E MULLER, HW BRASS, N TRENT, JM BLIN, N AF MEESE, E MULLER, HW BRASS, N TRENT, JM BLIN, N TI ASSIGNMENT OF ALU-REPETITIVE SEQUENCES TO LARGE RESTRICTION FRAGMENTS FROM HUMAN-CHROMOSOME-6 AND HUMAN-CHROMOSOME-22 SO MOLECULAR BIOLOGY REPORTS LA English DT Article DE PFGE (PULSED FIELD GEL ELECTROPHORESIS); REPETITIVE DNA; CHROMOSOMAL SEQUENCES; MAPPING ID MAPPING PANEL; SHORT ARM; MENINGIOMA; MELANOMA; DELETION; REPEATS; PROBES; LOCUS; MAP; PCR AB We have employed a pulsed field gel electrophoresis and Alu hybridization approach for identification of large restriction fragments on chromosome 6 and 22. This technique allows large portions of selected human chromosomes to be visualized as discrete hybridization signals. Somatic cell hybrid DNA which contains chromosome 6 or chromosome 22 was restricted. with either Notl or Mlul. The restriction fragments were separated by pulsed field gel electrophoresis (PFGE) and hybridized against an Alu repetitive sequence (Blur 8). The hybridization signals result in a fingerprint-like pattern which is unique for each chromosome and each restriction enzyme. In addition, a continuous pattern of restriction fragments was demonstrated by gradually increasing puls times. This approach will also be suitable to analyze aberrant human chromosomes retained in somatic cell hybrids and can be used to analyze flaw sorted human chromosomes. To this end, our method provides a valuable alternative to standard cytogenetic analysis. C1 NIH,NATL CTR HUMAN GENOME RES,BETHESDA,MD 20892. UNIV TUBINGEN,DIV MOLEC GENET,D-72074 TUBINGEN,GERMANY. RP MEESE, E (reprint author), MED SCH HOMBURG,INST HUMAN GENET,BAU 68,D-66421 HOMBURG,GERMANY. NR 24 TC 0 Z9 0 U1 1 U2 1 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0301-4851 J9 MOL BIOL REP JI Mol. Biol. Rep. PY 1995 VL 21 IS 2 BP 81 EP 84 DI 10.1007/BF00986496 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA RU981 UT WOS:A1995RU98100002 PM 8531924 ER PT J AU Margolis, N Samuels, DS AF Margolis, N Samuels, DS TI Proteins binding to the promoter region of the operon encoding the major outer surface proteins OspA and OspB of Borrelia burgdorferi SO MOLECULAR BIOLOGY REPORTS LA English DT Article DE Borrelia burgdorferi; DNA-binding protein; Lyme disease; outer membrane protein; transcriptional regulation ID LYME-DISEASE SPIROCHETE; MOLECULAR ANALYSIS; POLYMORPHISMS; ANTIBODY; TICKS AB The synthesis of the major outer surface proteins OspA and OspB in Borrelia burgdorferi varies among strains and during in vitro cultivation. We examined B. burgdorferi CA-11.2A for the presence of proteins that bind to the ospAB operon promoter region. Three major DNA-protein complexes were detected using a mobility shift assay; one of these complexes was due to sequence-specific binding. These proteins may be involved in the regulation of ospAB transcription and the pathogenesis of Lyme disease. C1 UNIV MONTANA,DIV BIOL SCI,MISSOULA,MT 59812. NIAID,ROCKY MT LABS,MICROBIAL STRUCT & FUNCT LAB,HAMILTON,MT 59840. NIAID,ROCKY MT LABS,BACTERIAL PATHOGENESIS SECT,HAMILTON,MT 59840. RI Samuels, D Scott/B-7549-2012 OI Samuels, D Scott/0000-0001-8352-7593 NR 29 TC 18 Z9 18 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0301-4851 J9 MOL BIOL REP JI Mol. Biol. Rep. PY 1995 VL 21 IS 3 BP 159 EP 164 DI 10.1007/BF00997238 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TZ578 UT WOS:A1995TZ57800004 PM 8832904 ER PT J AU STRAUSS, KI SCHULKIN, J JACOBOWITZ, DM AF STRAUSS, KI SCHULKIN, J JACOBOWITZ, DM TI CORTICOSTERONE EFFECTS ON RAT CALRETININ MESSENGER-RNA IN DISCRETE BRAIN NUCLEI AND THE TESTES SO MOLECULAR BRAIN RESEARCH LA English DT Article DE PARAVENTRICULAR HYPOTHALAMIC NUCLEUS; MEDIAL AMYGDALA; NUCLEUS REUNIENS; TESTOSTERONE; RNASE PROTECTION; RADIOIMMUNOASSAY ID CORTICOTROPIN-RELEASING-FACTOR; PARAVENTRICULAR NUCLEUS; GLUCOCORTICOID TOXICITY; NEURONAL DEATH; MESSENGER-RNA; KAINIC ACID; HIPPOCAMPUS; HORMONE; CELLS; CALBINDIN-D28K AB Calretinin is an EF-hand calcium binding protein found predominantly in discrete sets of neurons in the central system, and in the sex hormone producing cells of the gonads. Calretinin mRNA levels were measured in discrete brain areas from vehicle and corticosterone treated rats (subcutaneous injections of 0, 0.1, 1, or 10 mg, 7 days) using a micropunch ribonuclease protection assay. Treatment with high dose corticosterone (10 mg) caused a 93% decrease in calretinin mRNA levels in the hypothalamic paraventricular nucleus compared to controls. Two other brain regions, the medial amygdaloid nucleus and the nucleus reuniens, demonstrated an approximately 40% decrease in calretinin mRNA following high dose corticosterone. In separate experiments, adrenalectomy and diurnal corticosterone variations had no effect on calretinin mRNA in the brain areas examined. In the testes, corticosterone treatment decreased calretinin protein in a dose dependent fashion (to 81%, 68%, and 39% of controls at doses of 10, 1, and 0.1 mg/day, respectively). Low dose corticosterone treatments decreased testicular but not neuronal calretinin mRNA, whereas high dose corticosterone reduced calretinin mRNA in testes and several discrete brain areas. This suggests that corticosterone's effects on brain calretinin may be due to its pathological effects, e.g. energy depletion of brain cells or interference with the normal support functions of glia. C1 NIMH,BEHAV NEURSCI LAB,BETHESDA,MD 20892. RP STRAUSS, KI (reprint author), NIMH,CLIN SCI LAB,BLDG 10,ROOM 3D-48,BETHESDA,MD 20892, USA. NR 40 TC 15 Z9 15 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1995 VL 28 IS 1 BP 81 EP 86 DI 10.1016/0169-328X(94)00186-I PG 6 WC Neurosciences SC Neurosciences & Neurology GA QA521 UT WOS:A1995QA52100010 ER PT J AU JUCKER, M MONDADORI, C MOHAJERI, H BARTSCH, U SCHACHNER, M AF JUCKER, M MONDADORI, C MOHAJERI, H BARTSCH, U SCHACHNER, M TI TRANSIENT UP-REGULATION OF NCAM MESSENGER-RNA IN ASTROCYTES IN RESPONSE TO ENTORHINAL CORTEX LESIONS AND ISCHEMIA SO MOLECULAR BRAIN RESEARCH LA English DT Article DE NEURAL CELL ADHESION MOLECULE; TRANSIENT ISCHEMIA; ENTORHINAL CORTEX LESION; NEURONAL SPROUTING; SYNAPTOGENESIS; REACTIVE ASTROCYTE; HIPPOCAMPUS; RAT; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION ID FIBRILLARY ACIDIC PROTEIN; MOLECULE N-CAM; LAMININ-BINDING PROTEIN; CELL-CELL-INTERACTIONS; NERVE GROWTH-FACTOR; ADHESION MOLECULES; NEURITE OUTGROWTH; DENTATE GYRUS; REACTIVE ASTROCYTES; MOUSE AB Axonal sprouting and synaptic reorganization play an important role in the adaptation of the CNS to injury. However, the molecular mechanisms underlying this neuronal plasticity are poorly understood. In the present study we used in situ hybridization to examine the expression of NCAM mRNA in normal hippocampus, and in response to entorhinal cortex (EC) lesions and transient global ischemia. Both neurons and astrocytes were labeled by digoxygenin-tagged cRNA probes which recognize all three major NCAM isoforms of the adult CNS. In contrast, NCAM180-specific probes labeled only neurons in the hippocampus. After unilateral EC lesion, a transient and anatomically restricted upregulation of NCAM120/140 mRNA in reactive astrocytes in the denervated molecular layer of the dentate gyrus was observed. This increase was only present 2-4 days after the lesion whereas the GFAP mRNA increase was present up to 30 days postlesion. Following global ischemia a similar, transient increase of NCAM120/140 mRNA labeling of reactive astrocytes was observed; this increase was anatomically restricted to CA1, where neuronal loss occurred. Results suggest that the transient upregulation of NCAM120/140 mRNA in reactive astrocytes shortly after injury might be an important molecular mechanism in the cascade of events underlying neuronal plasticity in the adult CNS. C1 CIBA GEIGY LTD,DIV PHARMACEUT RES,BASEL,SWITZERLAND. ETH ZURICH,DEPT NEUROBIOL,ZURICH,SWITZERLAND. RP JUCKER, M (reprint author), NIA,GERONTOL RES CTR,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 42 TC 49 Z9 49 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JAN PY 1995 VL 28 IS 1 BP 149 EP 156 DI 10.1016/0169-328X(94)00206-T PG 8 WC Neurosciences SC Neurosciences & Neurology GA QA521 UT WOS:A1995QA52100020 ER PT J AU EYLER, YL SIWARSKI, DF HUPPI, KE LEWIS, AM AF EYLER, YL SIWARSKI, DF HUPPI, KE LEWIS, AM TI A P53 MUTATION IN EXON-5 ASSOCIATED WITH ADENOVIRUS TRANSFORMATION SO MOLECULAR CARCINOGENESIS LA English DT Note ID HUMAN PAPILLOMAVIRUS TYPE-16; PROTEINS; GENE; CANCERS; CELLS; DNA AB The adenovirus type 5 (Ad5) 55-kDa E1B oncoprotein has been shown to form complexes with the p53 tumor suppressor protein. These complexes are thought to interfere with normal p53 activity and may be responsible for the paucity of p53 mutations in cells transformed by these viruses. This report describes an example of a p53 mutation in exon 5 in an Ad5-transformed cell line that exhibited less expression of E1B 55-kDa protein and a longer tumor-latency phenotype than another Ad5-transformed cell line expressing wild-type p53. The finding of a p53 mutation in an Ad5-transformed cell line is unusual, especially considering the current theory that p53-E1B interactions play an important role in adenovirus transformation. This mutation could represent an alternative method of inactivating p53 function in the absence of sufficient levels of E1B 55-kDa oncoprotein. (C) 1995 Wiley-Liss, Inc. C1 NIAID,IMMUNOPATHOL LAB,BETHESDA,MD 20892. NCI,GENET LAB,MOLEC GENET SECT,BETHESDA,MD 20892. NR 16 TC 1 Z9 1 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PD JAN PY 1995 VL 12 IS 1 BP 1 EP 6 DI 10.1002/mc.2940120102 PG 6 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA QQ422 UT WOS:A1995QQ42200001 PM 7818760 ER PT J AU BRINKMANN, U BRINKMANN, E PASTAN, I AF BRINKMANN, U BRINKMANN, E PASTAN, I TI EXPRESSION CLONING OF CDNAS THAT RENDER CANCER-CELLS RESISTANT TO PSEUDOMONAS AND DIPHTHERIA-TOXIN AND IMMUNOTOXINS SO MOLECULAR MEDICINE LA English DT Article ID EXOTOXIN-A; GENE AMPLIFICATION; ASSAY; B3 AB Background: Several immunotoxins in which antibodies are coupled to plant or bacterial toxins are now in clinical trials for the treatment of cancer. One of these is B3-LysPE38 in which MAb B3 which reacts with many human cancers, is coupled with a genetically modified form of Pseudomonas exotoxin (PE). Materials and Methods: To investigate how cells can become resistant to PE-derived immunotoxins, we constructed an immunotoxin-sensitive MCF-7 breast cancer cell line that contains SV40 T antigen and allows episomal replication of SV40 origin containing plasmids. We transfected a pCDM8/HeLa cDNA expression library into these cells, thereby causing overexpression of the plasmid-encoded genes. The transfected cells were treated with immunotoxin to select for resistance-mediating plasmids, which were reisolated from these cells and amplified in Escherichia coli. The resulting plasmid pool was transfected into cells for two further rounds of selection and plasmid reisolation. Results: Several plasmids that caused immunotoxin resistance were enriched by this selection procedure. Four plasmids were stably transfected into MCF-7 cells and found to increase their resistance to PE-derived immunotoxins by 5- to 20-fold. These plasmids also confer resistance to native PE and to diphtheria toxin but not to ricin or cycloheximide. Thus, they appear to specifically interfere with the action of ADP-ribosylating toxins. Conclusion: Cancer cells can become resistant to immunotoxins by deregulated expression of normal genes. The clinical significance of this type of resistance will be evaluated in clinical trials. C1 NCI,DIV CANC BIOL DIAG & CTR,MOLEC BIOL LAB,BETHESDA,MD 20892. NICHHD,MOLEC GROWTH REGULAT LAB,BETHESDA,MD 20892. NR 37 TC 39 Z9 40 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 1076-1551 J9 MOL MED JI Mol. Med. PD JAN PY 1995 VL 1 IS 2 BP 206 EP 216 PG 11 WC Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine GA QX599 UT WOS:A1995QX59900011 PM 8529099 ER PT J AU PARSEGIAN, VA AF PARSEGIAN, VA TI THE COWS OR THE FENCE SO MOLECULAR MEMBRANE BIOLOGY LA English DT Editorial Material ID PHOSPHOLIPIDS; BILAYERS C1 NIDDK,BETHESDA,MD 20892. RP PARSEGIAN, VA (reprint author), DCRT,BETHESDA,MD 20892, USA. NR 6 TC 9 Z9 10 U1 1 U2 4 PU TAYLOR & FRANCIS LTD LONDON PI LONDON PA ONE GUNDPOWDER SQUARE, LONDON, ENGLAND EC4A 3DE SN 0968-7688 J9 MOL MEMBR BIOL JI Mol. Membr. Biol. PD JAN-MAR PY 1995 VL 12 IS 1 BP 5 EP 7 DI 10.3109/09687689509038488 PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QK661 UT WOS:A1995QK66100003 PM 7767382 ER PT J AU KOENIG, BW BERGELSON, LD GAWRISCH, K WARD, J FERRETTI, JA AF KOENIG, BW BERGELSON, LD GAWRISCH, K WARD, J FERRETTI, JA TI EFFECT OF THE CONFORMATION OF A PEPTIDE FROM GP41 ON BINDING AND DOMAIN FORMATION IN MODEL MEMBRANES SO MOLECULAR MEMBRANE BIOLOGY LA English DT Article; Proceedings Paper CT Fogarty-International-Center Conference on Domain Organization in Biological Membranes CY MAR 02-04, 1994 CL BETHESDA, MD SP FOGARTY INT CTR DE CONFORMATION; DYNAMIC EQUILIBRIUM; PEPTIDE-LIPID INTERACTION; NMR ID HELIX-COIL TRANSITION; ENVELOPE GLYCOPROTEIN; THERMODYNAMIC PARAMETERS; POLYPEPTIDE CHAINS; LIPID BILAYERS; BASIC PEPTIDES; ACIDIC LIPIDS; SEGMENTS; PROTEIN AB Binding of the peptide fragment 828-848 (P828), amino acid sequence RVIEVVQGACRAIRHIPRRIR, from the carboxy-terminal region of the envelope glycoprotein gp41 of human immunodeficiency virus type 1 (HIV-1) to membranes composed of a mixture of neutral and negatively charged phospholipids results in domain or cluster formation of the charged lipid. The conformation and dynamics of the peptide are investigated in solution and in the presence of sodium dodecyl sulphate (SDS) micelles using high resolution nuclear magnetic resonance (NMR) spectroscopy and circular dichroism (CD) spectropolarimetry. The CD results demonstrate that addition of either SDS, negatively charged phospholipid liposomes, or trifluoroethanol (TFE) induces a conformational transition of the peptide from a random coil or an extended chain in water to a more ordered structure with an estimated helical content of up to 60%. The structure of the peptide in a membrane mimetic SDS solution was investigated in detail using two-dimensional NMR. The measurements demonstrate the existence of a helical component in the peptide conformation in the SDS bound state. The peptide most likely exists as an ensemble of conformations with exchange times between them which are fast on the chemical shift NMR time scale (10(-3) s). Simple neutralization of the six arginine sidechain charges does not cause the peptide to adopt an ordered structure. Thus, there is an additional requirement for the structural transition such as that resulting from constraint of the peptide on a surface, or localization of the peptide at the lipid-water interface where the polarity is lower. Our results favour a model of the peptide-lipid interaction in which the peptide backbone is located in the water phase and part of the amino acid sidechains penetrate the lipid-water interface. C1 NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. HEBREW UNIV JERUSALEM,SCH PHARM,IL-91120 JERUSALEM,ISRAEL. NIAAA,MEMBRANE BIOCHEM & BIOPHYS LAB,ROCKVILLE,MD 20852. RI Koenig, Bernd/B-4315-2008 OI Koenig, Bernd/0000-0002-5300-6276 NR 22 TC 13 Z9 13 U1 2 U2 2 PU TAYLOR & FRANCIS LTD LONDON PI LONDON PA ONE GUNDPOWDER SQUARE, LONDON, ENGLAND EC4A 3DE SN 0968-7688 J9 MOL MEMBR BIOL JI Mol. Membr. Biol. PD JAN-MAR PY 1995 VL 12 IS 1 BP 77 EP 82 DI 10.3109/09687689509038499 PG 6 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QK661 UT WOS:A1995QK66100014 PM 7767387 ER PT J AU GAWRISCH, K BARRY, JA HOLTE, LL SINNWELL, T BERGELSON, LD FERRETTI, JA AF GAWRISCH, K BARRY, JA HOLTE, LL SINNWELL, T BERGELSON, LD FERRETTI, JA TI ROLE OF INTERACTIONS AT THE LIPID-WATER INTERFACE FOR DOMAIN FORMATION SO MOLECULAR MEMBRANE BIOLOGY LA English DT Article; Proceedings Paper CT Fogarty-International-Center Conference on Domain Organization in Biological Membranes CY MAR 02-04, 1994 CL BETHESDA, MD SP FOGARTY INT CTR DE DOMAIN; PHOSPHOLIPID; LIPID-PEPTIDE INTERACTION; HYDRATION; HEXAGONAL PHASE ID PHASE-TRANSITIONS; PHOSPHOLIPID HYDRATION; MAGNETIC-RESONANCE; MEMBRANES; MIXTURES; BILAYERS; STRESS; FORCES AB The lipid-water interface is critical for the packing of lipid molecules in membranes, We have demonstrated that lateral phase separation in membranes can be driven by electrostatic interactions such as those involving charged lipid species and oppositely charged peptides, in addition to hydration effects at the lipid-water interface. By using nuclear magnetic resonance (NMR), circular dichroism and fluorescence spectroscopy we have shown that binding of a 21-amino acid peptide containing six positively charged arginine residues to mixed phosphatidylcholine (PC)/phosphatidylglycerol (PG) membranes results in a conformational change in the peptide from a random coil to a helical structure and causes the formation of domains of negatively charged PG. Binding of the peptide to PG membranes disorders the lipid hydrocarbon chains. The strength of lipid-peptide binding at the interface, the conformational change in the peptide, and domain formation with the negatively charged lipid are coupled energetically. The lipid-peptide association constant is lower for membranes containing 20 mol% Po in PC/PG mixtures than for 100% PG membranes. We suggest that one of the factors that lower the association constant in PC/PG membranes is entropic energy of formation of PG domains. Besides electrostatic interactions, hydration of lipids is important for domain formation. We have shown that dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylethanolamine separate under conditions of decreased water activity. Furthermore, water activity controls lipid packing stress in the hydrocarbon core and the headgroups of membranes as demonstrated by induction of an inverse-hexagonal-to-lamellar phase transition in dioleoylphosphatidylethanolamine. The experiments have shown that lipid-peptide and lipid-water interactions at the interface influence the packing of lipid hydrocarbon chains. Consequently we predict that a change in lipid-lipid interaction in the hydrocarbon core of the membrane, for example as a result of the introduction of polyunsaturated fatty acids, will alter lipid-solvent and lipid-peptide interactions al the interface. C1 HEBREW UNIV JERUSALEM,DEPT BIOCHEM,JERUSALEM,ISRAEL. NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892. RP GAWRISCH, K (reprint author), NIAAA,MEMBRANE BIOCHEM & BIOPHYS LAB,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 20 TC 39 Z9 39 U1 0 U2 4 PU TAYLOR & FRANCIS LTD LONDON PI LONDON PA ONE GUNDPOWDER SQUARE, LONDON, ENGLAND EC4A 3DE SN 0968-7688 J9 MOL MEMBR BIOL JI Mol. Membr. Biol. PD JAN-MAR PY 1995 VL 12 IS 1 BP 83 EP 88 DI 10.3109/09687689509038500 PG 6 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QK661 UT WOS:A1995QK66100015 PM 7767388 ER PT J AU SALEM, N NIEBYLSKI, CD AF SALEM, N NIEBYLSKI, CD TI THE NERVOUS-SYSTEM HAS AN ABSOLUTE MOLECULAR-SPECIES REQUIREMENT FOR PROPER FUNCTION SO MOLECULAR MEMBRANE BIOLOGY LA English DT Article; Proceedings Paper CT Fogarty-International-Center Conference on Domain Organization in Biological Membranes CY MAR 02-04, 1994 CL BETHESDA, MD SP FOGARTY INT CTR DE DOCOSAHEXAENOIC ACID (22, 6N3); POLYUNSATURATES; TIME-RESOLVED FLUORESCENCE ANISOTROPY; PHOSPHOLIPID MOLECULAR SPECIES ID FATTY-ACID COMPOSITION; BRAIN PHOSPHATIDYLSERINES; RAT-BRAIN; CHROMATOGRAPHY; MEMBRANE AB A considerable body of biological evidence has accumulated that suggests that docosahexaenoic acid (22:6n3) is an essential component in the nervous system. Moreover, it appears from these studies that long chain polyunsaturates of the n-6 family such as arachidonate (20:4n6) and docosapentaenoate (22:5n6) cannot substitute for 22:6n3. This evidence is briefly reviewed and two hypotheses centering upon either biochemical or biophysical aspects of polyunsaturate function are presented and discussed. It is concluded that a bioactive metabolite of 22:6n3 is not responsible for its function in brain and that the best hypothesis asserts that a membrane function of a 22:6n3-containing species of phospholipid, such as phosphatidylserine, is critical for optimal neural function. Moreover, data are presented indicating that the biophysical properties of various highly unsaturated species of phospholipid are distinguishable. it is further contended that these species are not randomly distributed in membranes and thus the differences in physical properties may be amplified. It is concluded that a conceptual framework is needed in which the distinct membrane roles of phospholipid species may be understood as a function of the positions and numbers of double bonds. Only then may the critical role of the highly unsaturated n-3 polyunsaturates in the brain and retina be understood. RP SALEM, N (reprint author), NIAAA,MEMBRANE BIOCHEM & BIOPHYS LAB,ROCKVILLE,MD 20852, USA. NR 21 TC 109 Z9 113 U1 0 U2 0 PU TAYLOR & FRANCIS LTD LONDON PI LONDON PA ONE GUNDPOWDER SQUARE, LONDON, ENGLAND EC4A 3DE SN 0968-7688 J9 MOL MEMBR BIOL JI Mol. Membr. Biol. PD JAN-MAR PY 1995 VL 12 IS 1 BP 131 EP 134 DI 10.3109/09687689509038508 PG 4 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QK661 UT WOS:A1995QK66100023 PM 7767372 ER PT J AU BLUMENTHAL, R PAK, CC RAVIV, Y KRUMBIEGEL, M BERGELSON, LD MORRIS, SJ LOWY, RJ AF BLUMENTHAL, R PAK, CC RAVIV, Y KRUMBIEGEL, M BERGELSON, LD MORRIS, SJ LOWY, RJ TI TRANSIENT DOMAINS INDUCED BY INFLUENZA HEMAGGLUTININ DURING MEMBRANE-FUSION SO MOLECULAR MEMBRANE BIOLOGY LA English DT Article; Proceedings Paper CT Fogarty-International-Center Conference on Domain Organization in Biological Membranes CY MAR 02-04, 1994 CL BETHESDA, MD SP FOGARTY INT CTR DE MEMBRANE; FUSION; VIRAL; INFLUENZA; FLUORESCENCE ID SENDAI VIRUS GLYCOPROTEINS; VIRAL ENVELOPE PROTEIN; INDUCED CELL-FUSION; ROTATIONAL MOBILITY; SPIKE GLYCOPROTEINS; INITIAL-STAGES; LOW PH; HEMAGGLUTININ; FLUORESCENCE; PORE AB During low pH-induced fusion of influenza virus with erythrocytes we have observed differential dispersion of viral lipid and haemagglutinin (HA) into the erythrocyte membrane, and viral RNA into the erythrocyte using fluorescence video microscopy. The movement of both viral lipid and HA from virus to cell was restricted during the initial stages of fusion relative to free diffusion. This indicates the existence of relatively long-lived barriers to diffusion subsequent to fusion pore formation. Fluorescence anisotropy of phospholipid analogues incorporated into the viral membrane decreased when the pH was lowered to levels required for optimum fusion. This indicates that the restricted motion of viral membrane components was not due to rigidification of membrane lipids. The movement of HA from the fusion site was also assessed by photosensitized labelling by means of a fluorescent substrate (NBD-taurine) passing through the band 3 sialoglycoprotein (the erythrocyte anion transporter). We also examined the flow of lipid and aqueous markers during fusion of HA-expressing cells with labelled erythrocytes. During this cell-cell fusion, movement of lipid between fusing membranes begins before the fusion pore is wide enough to allow diffusion of aqueous molecules (M(r)>500). The data indicate that HA is capable of creating domains in the membrane and controlling continuity of aqueous compartments which are bounded by such domains. C1 NIDDK, CELL BIOL & GENET LAB, BETHESDA, MD 20892 USA. HUMBOLDT UNIV BERLIN, INST BIOL BIOPHYS, D-10115 BERLIN, GERMANY. HEBREW UNIV JERUSALEM, SCH PHARM, IL-91120 JERUSALEM, ISRAEL. UNIV MISSOURI, SCH BASIC LIFE SCI, DIV MOLEC BIOL & BIOCHEM, KANSAS CITY, MO 64110 USA. ARMED FORCES RADIOBIOL RES INST, DEPT PHYSIOL, BETHESDA, MD 20889 USA. RP BLUMENTHAL, R (reprint author), NCI, BLDG 10, ROOM 4A01, BETHESDA, MD 20892 USA. NR 41 TC 22 Z9 23 U1 0 U2 0 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0968-7688 EI 1464-5203 J9 MOL MEMBR BIOL JI Mol. Membr. Biol. PD JAN-MAR PY 1995 VL 12 IS 1 BP 135 EP 142 DI 10.3109/09687689509038509 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QK661 UT WOS:A1995QK66100024 PM 7767373 ER PT J AU BENYA, RV KUSUI, T PRADHAN, TK BATTEY, JF JENSEN, RT AF BENYA, RV KUSUI, T PRADHAN, TK BATTEY, JF JENSEN, RT TI EXPRESSION AND CHARACTERIZATION OF CLONED HUMAN BOMBESIN RECEPTORS SO MOLECULAR PHARMACOLOGY LA English DT Article ID GASTRIN-RELEASING PEPTIDE; CANCER CELL-LINES; NEUROMEDIN-B; HIGH-AFFINITY; MUSCLE-CELLS; 3T3 CELLS; GROWTH; CALCIUM; BINDING; IDENTIFICATION AB Little is known about the pharmacology or cell biology of human bombesin (Bn) receptors, because they are usually present at low levels and both subtypes are frequently present in the same tissues. Human gastrin-releasing peptide (GRP) receptors (huGRP-R) and human neuromedin B (NMB) receptors (huNMB-R) were stably transfected into BALB/3T3 fibroblasts. Both receptor types were glycosylated, with 35% of the huGRP-R and 38% of the huNMB-R representing carbohydrate residues. The extent of glycosylation of the transfected huGRP-R was the same as that seen in the human glioblastoma cell line U-118. Radiolabeled agonist ligands were rapidly internalized, whereas nonintemalized ligand readily dissociated in a temperature-dependent fashion. The affinities of various agonists for binding to the huGRP-R were Bn (K-i = 1.4 +/- 0.2 nM) = 4 x GRP = 300 x NMB. In contrast, affinities for the huNMB-R were NMB (K-i = 8.1 +/- 5.2 nM) = 4 x Bn = 600 x GRP. [F-5-D-Phe(6),D-Ala(11)]Bn(6-13)methyl eater was the most potent huGRP-R antagonist, whereas D-Nal-Cys-Tyr-D-Trp-Lys-Val-Cys-Nal-NH2 was the most potent huNMB-R antagonist. Agonist binding to either receptor type caused activation of phospholipase C and increased cellular [H-3]inositol phosphate levels. GRP was potent at increasing [H-3]inositol phosphate generation in cells expressing the huGRP-R (EC(50) = 13.6 +/- 1.3 nM), whereas NMB was similarly potent when acting upon cells expressing the huNMB-R (EC(50) = 9.3 +/- 1.4 nM). However, neither receptor type, when stimulated with agonist, caused an increase in cAMP levels. These data show that stably transfected huGRP-R exhibit similar pharmacology for agonists and antagonists, are appropriately glycosylated, and function similarly with respect to their ability to alter biological activity, compared with natively expressed receptors. Minimal native huNMB-R data are available for comparison, but in general the huNMB-R is similar to the rat NMB receptor in its pharmacology and cell biology. C1 NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. NCI,DEV THERAPEUT PROGRAM,BIOL CHEM LAB,BETHESDA,MD 20892. NR 42 TC 68 Z9 69 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD JAN PY 1995 VL 47 IS 1 BP 10 EP 20 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QE167 UT WOS:A1995QE16700002 PM 7838118 ER PT J AU BUCKPITT, A CHANG, AM WEIR, A VANWINKLE, L DUAN, XZ PHILPOT, R PLOPPER, C AF BUCKPITT, A CHANG, AM WEIR, A VANWINKLE, L DUAN, XZ PHILPOT, R PLOPPER, C TI RELATIONSHIP OF CYTOCHROME-P450 ACTIVITY TO CLARA CELL CYTOTOXICITY .4. METABOLISM OF NAPHTHALENE AND NAPHTHALENE OXIDE IN MICRODISSECTED AIRWAYS FROM MICE, RATS, AND HAMSTERS SO MOLECULAR PHARMACOLOGY LA English DT Article ID LIVER MICROSOMES; XENOBIOTIC METABOLISM; COVALENT BINDING; PULMONARY CELLS; LUNG; TOXICITY; RABBIT; MOUSE; IDENTIFICATION; MONOOXYGENASE AB Parenteral administration of naphthalene produces a dose-dependent and tissue-, species-, and cell-selective lesion of murine Clara cells. The rate and stereoselectivity of naphthalene metabolism by microsomal preparations correlate with tissue and species differences in cytotoxicity. Because earlier studies used microsomes obtained from whole tissue, differences in susceptibility of proximal and distal airways could not be related to differences in the metabolic activation or detoxication of naphthalene. Specific subcompartments of the respiratory system, obtained by microdissection, have been used to study the cytochrome P450-dependent metabolism of naphthalene and the epoxide hydrolase/glutathione transferase-dependent metabolism of naphthalene oxide. The rates of naphthalene metabolism were substantially higher in mouse airways than in comparable airways of hamsters or rats. Rates of metabolism were higher in distal airways than in the trachea of all species studied. Metabolism in mouse airways was highly stereoselective, whereas that in hamster and rat tissues was not. Nonciliated cells at all airway levels in mice were heavily labeled with an antibody to cytochrome P450 2F2; little labeling was observed in any portion of rat and hamster lungs. Postmitochondrial supernatants prepared from mouse and hamster airways metabolized racemic naphthalene oxide to diet and glutathione adducts at substantially higher rates than did comparable preparations from rats. Although glutathione levels varied 2-4-fold at different airway levels in the three species studied, levels at the most susceptible site (mouse distal bronchioles) were as high as or higher than those at other, less susceptible, sites. These studies support the view that the rate and stereoselectivity of naphthalene metabolism to naphthalene 1R,2S-oxide catalyzed by cytochrome P450 2F2 are critical determinants in the species-specific and region-selective cytotoxicity of naphthalene in mice. The lack of major differences in the catalytic activity or enantioselectivity of putative detoxication enzymes (epoxide hydrolase or glutathione transferases) between mouse and hamster tissue, combined with data showing that the differences in the metabolic fate of naphthalene oxide in proximal versus distal airways are not dramatic, suggests that the initial epoxidation of naphthalene is an important factor in site-selective toxicity. These studies support the need to use tissue from defined airway levels for studies on the relationship of biochemical and metabolic factors important in cellular injury by lung toxicants, such as naphthalene, where there are dramatic regional differences in susceptibility to injury within the respiratory system. C1 UNIV CALIF DAVIS,SCH VET MED,DEPT ANAT,DAVIS,CA 95616. UNIV CALIF DAVIS,SCH VET MED,DEPT PHYSIOL & CELL BIOL,DAVIS,CA 95616. UNIV CALIF DAVIS,SCH MED,OCCUPAT & ENVIRONM HLTH UNIT,DAVIS,CA 95616. NIEHS,CELL & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. RP BUCKPITT, A (reprint author), UNIV CALIF DAVIS,SCH VET MED,DEPT MOLEC BIOSCI,DAVIS,CA 95616, USA. NR 34 TC 126 Z9 128 U1 0 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0026-895X J9 MOL PHARMACOL JI Mol. Pharmacol. PD JAN PY 1995 VL 47 IS 1 BP 74 EP 81 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QE167 UT WOS:A1995QE16700009 PM 7838135 ER PT J AU HORWITZ, MS TUFARIELLO, J GRUNHAUS, A FEJER, G AF HORWITZ, MS TUFARIELLO, J GRUNHAUS, A FEJER, G TI MODEL SYSTEMS FOR STUDYING THE EFFECTS OF ADENOVIRUS E3 GENES ON VIRULENCE IN-VIVO SO MOLECULAR REPERTOIRE OF ADENOVIRUSES III SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Review ID TUMOR-NECROSIS-FACTOR; GENERALIZED VIRAL INFECTION; SEPARATE E1A DOMAINS; HUMAN LYMPHOID-CELLS; FACTOR-ALPHA; VACCINIA VIRUS; REGION E3; PROTEIN; PATHOGENESIS; RECEPTOR C1 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT CELL BIOL,BRONX,NY 10461. YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PEDIAT,BRONX,NY 10461. NIAID,VIRAL DIS LAB,BETHESDA,MD 20892. RP HORWITZ, MS (reprint author), YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MICROBIOL & IMMUNOL,1300 MORRIS PK AVE,BRONX,NY 10461, USA. RI Fejer, Gyorgy/D-5080-2011 FU NCI NIH HHS [P01 CA 13330]; NIAID NIH HHS [AI27199]; NIGMS NIH HHS [5T32 GM07288] NR 62 TC 19 Z9 19 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1995 VL 199 IS 3 BP 195 EP 211 PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology; Virology GA BE24P UT WOS:A1995BE24P00011 PM 7555077 ER PT J AU Posada, G Gao, Y Wu, F Posada, R Tascon, M Schoelmerich, A Sagi, A KondoIkemura, K Haaland, W Synnevaag, B AF Posada, G Gao, Y Wu, F Posada, R Tascon, M Schoelmerich, A Sagi, A KondoIkemura, K Haaland, W Synnevaag, B TI The secure-base phenomenon across cultures: Children's behavior, mothers' preferences, and experts' concepts SO MONOGRAPHS OF THE SOCIETY FOR RESEARCH IN CHILD DEVELOPMENT LA English DT Article C1 SUNY STONY BROOK,DEPT PSYCHOL,STONY BROOK,NY 11794. HOOD COLL,FREDERICK,MD 21701. INST COLOMBIAN BIENESTAR FAMILIAR,BOGOTA,COLOMBIA. UNIV BERGEN,BERGEN,NORWAY. NICHHD,BETHESDA,MD 20892. UNIV HAIFA,IL-31999 HAIFA,ISRAEL. UNIV JAVERIANA,DEPT PSYCHOL,BOGOTA,COLOMBIA. OSAKA UNIV,FAC HUMAN SCI,SUITA,OSAKA 565,JAPAN. RP Posada, G (reprint author), UNIV DENVER,DENVER,CO 80210, USA. RI Schoelmerich, Axel/C-9039-2009 OI Schoelmerich, Axel/0000-0002-9844-3920 NR 0 TC 68 Z9 70 U1 0 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0037-976X J9 MONOGR SOC RES CHILD JI Monogr. Soc. Res. Child Dev. PY 1995 VL 60 IS 2-3 BP 27 EP 48 DI 10.2307/1166169 PG 22 WC Psychology, Developmental SC Psychology GA TL970 UT WOS:A1995TL97000004 ER PT B AU LONG, LR BERMAN, LE NEVE, L ROY, G THOMA, GR AF LONG, LR BERMAN, LE NEVE, L ROY, G THOMA, GR BE Rodriguez, AA Maitan, J TI AN APPLICATION-LEVEL TECHNIQUE FOR FASTER TRANSMISSION OF LARGE IMAGES ON THE INTERNET SO MULTIMEDIA COMPUTING AND NETWORKING 1995 SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Multimedia Computing and Networking 1995 CY FEB 06-08, 1995 CL SAN JOSE, CA SP SOC IMAGING SCI & TECHNOL, SOC PHOTO OPT INSTRUMENTAT ENGINEERS, IEEE, COMP SOC, IEEE, COMMUN SOC DE INTERNET, SOCKETS; MEDICAL X-RAYS; IMAGES; IMAGE TRANSMISSION; CLIENT/SERVER; OPTICAL STORAGE C1 NATL LIB MED,BETHESDA,MD 20209. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1764-5 J9 P SOC PHOTO-OPT INS PY 1995 VL 2417 BP 116 EP 129 DI 10.1117/12.206077 PG 14 WC Computer Science, Theory & Methods SC Computer Science GA BC91L UT WOS:A1995BC91L00011 ER PT S AU STRIKER, G AF STRIKER, G BE Zderic, SA TI Extracellular matrix update - Overview SO MUSCLE, MATRIX, AND BLADDER FUNCTION SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT Symposium on Muscle, Matrix, and Bladder Function CY MAR 18-19, 1994 CL PHILADELPHIA, PA SP NIH, Smith Kline Beecham Pharm, Sandoz Pharm, Glaxo Res Inst, Ortho McNeil Pharm, Pfizer Res PLC, C R Bard Inc RP NATL INST DIGEST & KIDNEY DIS,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-45193-X J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 385 BP 141 EP 142 PG 2 WC Medicine, Research & Experimental; Pediatrics; Urology & Nephrology SC Research & Experimental Medicine; Pediatrics; Urology & Nephrology GA BE24N UT WOS:A1995BE24N00016 PM 8571824 ER PT S AU CORCORAN, ML KLEINER, DE STETLERSTEVENSON, WG AF CORCORAN, ML KLEINER, DE STETLERSTEVENSON, WG BE Zderic, SA TI Regulation of matrix metalloproteinases during extracellular matrix turnover SO MUSCLE, MATRIX, AND BLADDER FUNCTION SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT Symposium on Muscle, Matrix, and Bladder Function CY MAR 18-19, 1994 CL PHILADELPHIA, PA SP NIH, Smith Kline Beecham Pharm, Sandoz Pharm, Glaxo Res Inst, Ortho McNeil Pharm, Pfizer Res PLC, C R Bard Inc C1 NCI,PATHOL LAB,EXTRACELLULAR MATRIX PATHOL SECT,BETHESDA,MD 20892. RP CHILDRENS HOSP DARTMOUTH,DEPT PEDIAT,LEBANON,NH 03756, USA. RI Stetler-Stevenson, William/H-6956-2012; OI Stetler-Stevenson, William/0000-0002-5500-5808; Kleiner, David/0000-0003-3442-4453 NR 0 TC 27 Z9 27 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-45193-X J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 385 BP 151 EP 159 PG 9 WC Medicine, Research & Experimental; Pediatrics; Urology & Nephrology SC Research & Experimental Medicine; Pediatrics; Urology & Nephrology GA BE24N UT WOS:A1995BE24N00018 PM 8571826 ER PT J AU RABEN, N NICHOLS, RC BOERKOEL, C PLOTZ, P AF RABEN, N NICHOLS, RC BOERKOEL, C PLOTZ, P TI GENETIC-DEFECTS IN PATIENTS WITH GLYCOGENOSIS TYPE-II (ACID MALTASE DEFICIENCY) SO MUSCLE & NERVE LA English DT Article; Proceedings Paper CT International Symposium on Glycolytic and Mitochondrial Defects in Muscle and Nerve CY JUL 07-08, 1994 CL SUITA, JAPAN ID LYSOSOMAL ALPHA-GLUCOSIDASE; DISEASE TYPE-II; MESSENGER-RNA; MISSENSE MUTATION; POMPE DISEASE; ONE ALLELE; IDENTIFICATION; HETEROGENEITY; BIOSYNTHESIS; SUBSTITUTION AB Inherited deficiency of acid a-glucosidase (acid maltase, GAA) leads to glycogen storage disease type II. Clinical manifestations and prognosis of the disease depend on the age of onset and tissue involvement. GAA deficiency is extremely heterogeneous, ranging from a rapidly progressive fatal infantile-onset form to a slowly progressive adult-onset myopathy associated with respiratory insufficiency. Biochemical and immunochemical studies of the biosynthesis of the enzyme in GAA-deficient patients established the molecular diversity of the disease. Cloning and sequencing of the cDNA and the gene provided the basis for genetic analysis of the patients with different phenotypes. in this article, we summarize the data on mutations in the GAA gene and discuss the correlation between the genotype and phenotypic expression of the disease. (C) 1995 John Wiley and Sons, Inc. RP RABEN, N (reprint author), NIAMSD,ARTHRIT & RHEUMATISM BRANCH,10-9N244,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 39 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-639X J9 MUSCLE NERVE JI Muscle Nerve PY 1995 SU 3 BP S70 EP S74 PG 5 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA RE288 UT WOS:A1995RE28800013 ER PT J AU RABEN, N SHERMAN, JB ADAMS, E NAKAJIMA, H ARGOV, Z PLOTZ, P AF RABEN, N SHERMAN, JB ADAMS, E NAKAJIMA, H ARGOV, Z PLOTZ, P TI VARIOUS CLASSES OF MUTATIONS IN PATIENTS WITH PHOSPHOFRUCTOKINASE DEFICIENCY (TARUIS DISEASE) SO MUSCLE & NERVE LA English DT Article; Proceedings Paper CT International Symposium on Glycolytic and Mitochondrial Defects in Muscle and Nerve CY JUL 07-08, 1994 CL SUITA, JAPAN ID HUMAN-GENE; MUSCLE; ASSIGNMENT; CLONING AB Muscle phosphofructokinase (PFK-M) deficiency (glycogenosis type VII, Tarui's disease) is characterized by intolerance to vigorous exercise, often accompanied by myoglobinuria. The disease is inherited as an autosomal recessive trait. The clinical manifestations are similar to those in myophosphorylase deficiency (McArdle's disease), and the diagnosis required demonstration of the enzyme defect in muscle biopsy. In the Western hemisphere PFK deficiency appears to be prevalent among people of Ashkenazi Jewish descent. To define the molecular basis of this myopathy, we have studied 11 Ashkenazi and 2 non-Ashkenazi families with the disease. Ashkenazi patients share two common pathogenic mutations, a splicing defect and a nucleotide deletion, which account for similar to 95% of mutant alleles. The molecular diagnosis is now possible in this population by using simple PCR-based tests to screen for these mutations. (C) 1995 John Wiley and Sons, Inc. RP RABEN, N (reprint author), NIAMSD,ARTHRIT & RHEUMATISM BRANCH,10-9N244,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 22 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-639X J9 MUSCLE NERVE JI Muscle Nerve PY 1995 SU 3 BP S35 EP S38 PG 4 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA RE288 UT WOS:A1995RE28800007 ER PT B AU Standaert, FG AF Standaert, FG BE Fukushima, K Ochiai, R TI Nicotinic receptors SO MUSCLE RELAXANTS: PHYSIOLOGIC AND PHARMACOLOGIC ASPECTS LA English DT Proceedings Paper CT 5th International Neuromuscular Meeting CY NOV 17-20, 1994 CL TOKYO, JAPAN C1 UNIV MICHIGAN,NIMH,DEPT ANESTHESIOL,PERRYSBURG,OH 43551. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER-VERLAG PI TOKYO PA 37-3, HONGO 3-CHOME BONKYO-KU, TOKYO 113, JAPAN BN 4-431-70168-0 PY 1995 BP 31 EP 36 PG 6 WC Anesthesiology; Pharmacology & Pharmacy SC Anesthesiology; Pharmacology & Pharmacy GA BF38J UT WOS:A1995BF38J00005 ER PT J AU ENNIS, DG LEVINE, AS KOCH, WH WOODGATE, R AF ENNIS, DG LEVINE, AS KOCH, WH WOODGATE, R TI ANALYSIS OF RECA MUTANTS WITH ALTERED SOS FUNCTIONS SO MUTATION RESEARCH-DNA REPAIR LA English DT Article DE ESCHERICHIA COLI; SOS MUTAGENESIS; CHEMILUMINESCENT IMMUNOASSAY; UMUDC; UMUD CLEAVAGE ID SINGLE-STRANDED-DNA; ESCHERICHIA-COLI; CONSTITUTIVE EXPRESSION; MUTAGENESIS PROTEIN; MUCAB PROTEINS; LEXA CLEAVAGE; UMUD; MUTATIONS; INDUCTION; DEFICIENT AB The Escherichia coli RecA protein has at least three roles in SOS mutagenesis: (1) derepression of the SOS regulon by mediating LexA cleavage; (2) activation of the UmuD mutagenesis protein by mediating its cleavage; and (3) targeting the Umu-like mutagenesis proteins to DNA. Using a combined approach of molecular and physiological assays, it is now possible to determine which of the three defined steps has been altered in any recA mutant. In this study, we have focussed on the ability of six particular recA mutants (recA85, recA430, recA432, recA433, recA435 and recA730) to perform these functions. Phenotypically, recA85 and recA730 were similar in that in lexA(+) and lexA(Def) backgrounds, they exhibited constitutive coprotease activity towards the UmuD mutagenesis protein. Somewhat surprisingly, in a lexA(Ind(-)) background, UmuD cleavage was damage inducible, suggesting that the repressed level of the RecA* protein cannot spontaneously achieve a fully activated state. Although isolated in separate laboratories, the nucleotide sequence of the recA85 and recA730 mutants revealed that they were identical, with both alleles possessing a Glu(38) --> Lys change in the mutant protein. The recA430, recA433 and recA435 mutants were found to be defective for both lambda mutagenesis and UmuD cleavage. lambda mutagenesis was fully restored, however, to the recA433 and recA435 strains by a low copy plasmid expressing the mutagenically active UmuD' protein. In contrast, lambda mutagenesis was only partially restored to a recA430 strain by a high copy UmuD' plasmid, suggesting that RecA430 may also be additionally defective in targeting the Umu proteins to DNA. Sequence analysis of the recA433 and recA435 alleles revealed identical substitutions resulting in Arg(243) --> His. The recA432 mutation had a complex phenotype in that its coprotease activity towards UmuD depended upon the lexA background: inducible in lexA(+) strains, inefficient in lexA(Ind(-)) cells and constitutive in a lexA(Def) background. The recA432 mutant was found to carry a Pro(119) --> Ser substitution, a residue believed to be at the RecA subunit interface; thus this complex phenotype may result from alterations in the assembly of RecA multimers. C1 US FDA,MOLEC BIOL BRANCH,WASHINGTON,DC 20204. RP ENNIS, DG (reprint author), NICHHD,DNA REPLICAT REPAIR & MUTAGENESIS SECT,BETHESDA,MD 20892, USA. NR 53 TC 42 Z9 42 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8777 J9 MUTAT RES-DNA REPAIR JI Mutat. Res.-DNA Repair PD JAN PY 1995 VL 336 IS 1 BP 39 EP 48 DI 10.1016/0921-8777(94)00045-8 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology SC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology GA QG036 UT WOS:A1995QG03600005 PM 7528894 ER PT J AU WITT, KL GULATI, DK KAUR, P SHELBY, MD AF WITT, KL GULATI, DK KAUR, P SHELBY, MD TI PHENOLPHTHALEIN - INDUCTION OF MICRONUCLEATED ERYTHROCYTES IN MICE SO MUTATION RESEARCH-GENETIC TOXICOLOGY LA English DT Article DE MICRONUCLEUS; CYTOGENETICS; GENETIC TOXICITY; MUTAGEN; LAXATIVE ID HAMSTER OVARY CELLS; CHEMICALS; TOXICITY; TESTS AB Phenolphthalein was tested for the induction of micronucleated erythrocytes in mice. Results of an initial investigation revealed significant, dose-related increases in micronucleated polychromatic erythrocytes (MN-PCE) and normochromatic erythrocytes (MN-NCE) in peripheral blood samples of male and female mice exposed to 0.6% to 5% phenolphthalein (approximately 1100 to 10,000 mg/kg/day) in feed for 90 days (Dietz et al., 1992). Results from a second long-term feed study with Swiss CD-1 mice confirmed this effect. However, administration of comparable doses of phenolphthalein by corn oil gavage on two consecutive days gave negative results in a mouse bone marrow micronucleus test. Subsequent tests were performed to clarify the conflicting results seen in the chronic exposure, dosed-feed, peripheral blood studies and the acute, corn oil gavage, bone marrow studies. Phenolphthalein was administered to male B6C3F(1) mice in feed (3%) for 14 days. Peripheral blood samples taken at 4, 7, and 14 days all showed significant increases in micronucleated PCE; bone marrow samples taken on days 7 and 14 also were clearly positive for micronucleus induction. Therefore, comparable results were obtainable from both bone marrow and peripheral blood analyses. Because of the negative results in the two-exposure gavage test, additional tests were then designed to investigate the effects of bolus vs continuous dosing, feeding vs gavage administration, and corn oil vs feed as a carrier for phenolphthalein. Results of these tests indicated that the rate of exposure to phenolphthalein affects the frequency of induced MN-PCE and that micronucleated erythrocytes can be induced by phenorphthalein either by feeding or by corn ail gavage administration. In all the acute exposure studies, relatively high doses of phenolphthalein (2000-6000 mg/kg/day for at least 2 days) were required to induce micronuclei. The positive results obtained with phenolpthalein in vivo were consistent with the results of an in vitro chromosomal aberration test in Chinese hamster ovary cells, where dose-related increases in aberrations were noted only in cells treated in the presence of induced rat liver S9. C1 NIEHS,RES TRIANGLE PK,NC 27709. ENVIRONM HLTH RES & TESTING INC,LEXINGTON,KY 40503. OAK RIDGE INST SCI & EDUC,OAK RIDGE,TN 37831. NR 12 TC 25 Z9 26 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-1218 J9 MUTAT RES-GENET TOX JI Mutat. Res.-Genet. Toxicol. PD JAN PY 1995 VL 341 IS 3 BP 151 EP 160 DI 10.1016/0165-1218(95)90005-5 PG 10 WC Genetics & Heredity; Toxicology SC Genetics & Heredity; Toxicology GA QB880 UT WOS:A1995QB88000002 PM 7529356 ER PT J AU Evans, CH Lee, TS Flugelman, AA AF Evans, CH Lee, TS Flugelman, AA TI Spermine-directed immunosuppression of cervical carcinoma cell sensitivity to a majority of lymphokine activated killer lymphocyte cytotoxicity SO NATURAL IMMUNITY LA English DT Article DE spermine; lymphocyte; interferon; cytotoxicity ID HUMAN SEMINAL PLASMA; LEUKOREGULIN UP-REGULATION; NATURAL-KILLER; CYTO-TOXICITY; POLYAMINES; INVITRO; SERUM; PROLIFERATION; SUPPRESSION; ELUCIDATION AB The polyamine spermine, naturally present at millimolar levels in seminal plasma, inhibits proliferation of natural killer cells and T lymphocytes, directly binds to DNA and alters cervical cell ploidy indicating its potential contribution to the etiology of cervical cancer and its possible role in suppression of the destruction of dysplastic and neoplastic cervical epithelial cells by cytotoxic lymphocytes. This study demonstrates that spermine suppresses sensitivity of cervical carcinoma cells to lymphokine-activated killer (LAK) lymphocytes from more than half of normal individuals, Direct treatment of Cr-51-labelled QGU cervical carcinoma cells with greater than or equal to 10(-11) M spermine for 1 h reduces cytotoxic destruction of QGU cells by human LAK lymphocytes up to 60% (p less than or equal to 0.05) as shown by decreased LAK lymphocyte lytic units(20%). Cervical carcinoma cells are inherently very sensitive to LAK lymphocytes and spermine may be an important immunosuppressive agent in natural immunity against cervical cancer. RP Evans, CH (reprint author), NCI,DIV CANC ETIOL,BIOL LAB,NIH,BLDG 37,ROOM 2A17,BETHESDA,MD 20892, USA. NR 39 TC 7 Z9 9 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1018-8916 J9 NAT IMMUN JI Nat. Immun. PY 1995 VL 14 IS 3 BP 157 EP 163 PG 7 WC Cell Biology; Immunology SC Cell Biology; Immunology GA TU320 UT WOS:A1995TU32000005 PM 8832899 ER PT J AU RASHID, MA GUSTAFSON, KR CARDELLINA, JH BOYD, MR AF RASHID, MA GUSTAFSON, KR CARDELLINA, JH BOYD, MR TI BROMINATED CHAMIGRANE SESQUITERPENES PRODUCE A NOVEL PROFILE OF DIFFERENTIAL CYTOTOXICITY IN THE NCI IN-VITRO SCREEN SO NATURAL PRODUCT LETTERS LA English DT Article DE BROMINATED SESQUITERPENE; CHAMIGRANE; LAURENCIA MAJUSCULA ID VACUUM LIQUID-CHROMATOGRAPHY; TUMOR-CELL LINES; DIVERSE PANEL; MARINE-ALGAE; LAURENCIA; RHODOMELACEAE; SEPARATION; MIXTURES AB Bioassay-guided fractionation of an extract from the marine alga Laurencia majuscula provided three brominated chamigrane sesquiterpenes (1-3). These compounds produced a novel pattern of antitumor activity against the National Cancer Institute's in vitro cell line panel. They were cytotoxic to certain cell lines in the colon cancer subpanel at concentrations 10-100 fold lower than the mean cytotoxic concentration observed in the other tumor subpanels. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. NR 19 TC 17 Z9 17 U1 0 U2 2 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1057-5634 J9 NAT PROD LETT JI Nat. Prod. Lett. PY 1995 VL 6 IS 4 BP 255 EP 259 DI 10.1080/10575639508043168 PG 5 WC Biochemistry & Molecular Biology; Chemistry, Medicinal SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA RJ857 UT WOS:A1995RJ85700003 ER PT J AU HALLOCK, YF HUGHES, CB CARDELLINA, JH SCHAFFER, M GULDEN, KP AF HALLOCK, YF HUGHES, CB CARDELLINA, JH SCHAFFER, M GULDEN, KP TI DIONCOPHYLLINE-A, THE PRINCIPAL CYTOTOXIN FROM ANCISTROCLADUS-LETESTUI SO NATURAL PRODUCT LETTERS LA English DT Article DE DIONCOPHYLLINE A; NAPHTHYLISOQUINOLINE; ANCISTROCLADUS LETESTUI; CYTOTOXIN; OXIDATIVE DEGRADATION ID ACETOGENIC ISOQUINOLINE ALKALOIDS; TRIPHYOPHYLLINE; STEREOSTRUCTURE AB Using bioassay-guided fractionation, dioncophylline A [1] was isolated from the leaves and twigs of the tropical liana Ancistrocladus letestui Pelligr. Dioncophylline A was previously known from Triphyophyllum peltatum and A. abbreviatus. The in vitro cytotoxicity profile of 1 was characterized against the NCI panel of human tumor cell lines. C1 NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. NR 13 TC 11 Z9 11 U1 0 U2 1 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1057-5634 J9 NAT PROD LETT JI Nat. Prod. Lett. PY 1995 VL 6 IS 4 BP 315 EP 320 DI 10.1080/10575639508043177 PG 6 WC Biochemistry & Molecular Biology; Chemistry, Medicinal SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA RJ857 UT WOS:A1995RJ85700012 ER PT J AU FRANCOMANO, CA AF FRANCOMANO, CA TI KEY ROLE FOR A MINOR COLLAGEN SO NATURE GENETICS LA English DT Editorial Material C1 JOHNS HOPKINS UNIV, SCH MED, CTR GENET MED, BALTIMORE, MD 21287 USA. RP FRANCOMANO, CA (reprint author), NIH, NATL CTR HUMAN GENOME RES, BETHESDA, MD 20892 USA. NR 33 TC 20 Z9 20 U1 0 U2 1 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1995 VL 9 IS 1 BP 6 EP 8 DI 10.1038/ng0195-6 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA PZ913 UT WOS:A1995PZ91300004 PM 7704026 ER PT J AU TORO, C HALLETT, M ROTHWELL, JC ASSELMAN, PT MARSDEN, CD AF TORO, C HALLETT, M ROTHWELL, JC ASSELMAN, PT MARSDEN, CD TI PHYSIOLOGY OF NEGATIVE MYOCLONUS SO NEGATIVE MOTOR PHENOMENA SE ADVANCES IN NEUROLOGY LA English DT Article ID ASTERIXIS; EPILEPSY C1 INST NEUROL,MRC,HUMAN MOVEMENT & BALANCE UNIT,LONDON WC1N 3BC,ENGLAND. RP TORO, C (reprint author), NINCDS,HUMAN MOTOR CONTROL SECT,MED NEUROL BRANCH,BETHESDA,MD 20892, USA. OI Rothwell, John/0000-0003-1367-6467 NR 16 TC 11 Z9 12 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0091-3952 J9 ADV NEUROL JI Adv.Neurol. PY 1995 VL 67 BP 211 EP 217 PG 7 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA BE11Z UT WOS:A1995BE11Z00016 PM 8848971 ER PT J AU KAUR, J MARWAHA, SS BOYD, MR SODHI, GS AF KAUR, J MARWAHA, SS BOYD, MR SODHI, GS TI ORGANOMERCURY(II) COMPLEXES WITH ANTICARCINOGENIC AGENTS .2. ANTINEOPLASTIC ACTIVITY SO NEOPLASMA LA English DT Article DE ANTINEOPLASTIC EFFECTS; CELL LINES; CELL PANELS; ORGANOMERCURY AB Organomercury(II) complexes of the type, p-MeOC(6)H(4)HgL(1) (I), p-NO(2)C(6)H(4)HgL(2) (II), p-MeOC(6)H4(H)gL(3) (III) and p-MeC(6)H(4)HgL(4) (IV) (HL(1) - 6-mercaptopurine, HL(2)-6-thioguanine, HL(3)-5-fluorouracil, L(4) - phenyldithiocarbazate) have been screened against the following cell panels: leukemia, non-small cell lung cancer, small cell lung cancer, brain cancer, melanoma, ovarian cancer and renal cancer. The variation in anti-neoplastic activity has been correlated with the structural parameters of the complexes. C1 UNIV DELHI,SGTB KHALSA COLL,DEPT CHEM,DELHI 110007,INDIA. UNIV DELHI,DEPT CHEM,DELHI,INDIA. PUNJABI UNIV,DEPT BIOTECHNOL,PATIALA,PUNJAB,INDIA. NCI,BETHESDA,MD 20892. NR 13 TC 1 Z9 1 U1 0 U2 0 PU SLOVAK ACADEMIC PRESS LTD PI BRATISLAVA PA PO BOX 57, NAM SLOBODY 6, 810 05 BRATISLAVA, SLOVAKIA SN 0028-2685 J9 NEOPLASMA JI Neoplasma PY 1995 VL 42 IS 4 BP 191 EP 193 PG 3 WC Oncology SC Oncology GA RX177 UT WOS:A1995RX17700008 PM 7659185 ER PT J AU VANDENBRULE, FA BUICU, C SOBEL, ME LIU, FT CASTRONOVO, V AF VANDENBRULE, FA BUICU, C SOBEL, ME LIU, FT CASTRONOVO, V TI GALECTIN-3, A LAMININ-BINDING PROTEIN, FAILS TO MODULATE ADHESION OF HUMAN-MELANOMA CELLS TO LAMININ SO NEOPLASMA LA English DT Article DE LAMININ; ADHESION; GALECTIN-3; INVASION; METASTASIS; MELANOMA ID ENDOGENOUS LECTIN; COLON-CARCINOMA; TUMOR INVASION; MESSENGER-RNA; EXPRESSION; METASTASIS; RECEPTOR; IDENTIFICATION; TRANSFORMATION; MACROPHAGES AB Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and A375 melanoma cell expressed galectin-3 on their surface as demonstrated by immunofluorescence, and attached to laminin in an in vitro assay. We demonstrate that neither recombinant galectin-3 nor an affinity purified antigalectin-3 antiserum altered adhesion of A2058 or A375 melanoma cells to laminin. Our data strongly suggest that galectin-3 is not a key element in adhesion of the melanoma cells to laminin. These results are not surprising in light of the observation that galectin-3 expression is down regulated in cancer and that increased adhesion to laminin is a constant feature of invasive cancer cells. C1 NCI, PATHOL LAB, MOLEC PATHOL SECT, BETHESDA, MD 20892 USA. SCRIPPS RES INST, DEPT MOLEC & EXPTL MED, LA JOLLA, CA USA. RP VANDENBRULE, FA (reprint author), UNIV LIEGE, METASTASIS RES LAB, B-4000 LIEGE, BELGIUM. NR 39 TC 30 Z9 31 U1 1 U2 1 PU SLOVAK ACADEMIC PRESS LTD PI BRATISLAVA PA PO BOX 57, NAM SLOBODY 6, 810 05 BRATISLAVA, SLOVAKIA SN 0028-2685 J9 NEOPLASMA JI Neoplasma PY 1995 VL 42 IS 5 BP 215 EP 219 PG 5 WC Oncology SC Oncology GA TJ371 UT WOS:A1995TJ37100002 PM 8552198 ER PT J AU STRIKER, GE AF STRIKER, GE TI REPORT ON A WORKSHOP TO DEVELOP MANAGEMENT RECOMMENDATIONS FOR THE PREVENTION OF PROGRESSION IN CHRONIC RENAL-DISEASE BETHESDA (MA) APRIL-1994 SO NEPHROLOGY DIALYSIS TRANSPLANTATION LA English DT Editorial Material RP STRIKER, GE (reprint author), NIH,DIV KIDNEY UROL & HEMATOL DIS,BLDG 10,BETHESDA,MD 20892, USA. NR 9 TC 16 Z9 16 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0931-0509 J9 NEPHROL DIAL TRANSPL JI Nephrol. Dial. Transplant. PY 1995 VL 10 IS 2 BP 290 EP 292 PG 3 WC Transplantation; Urology & Nephrology SC Transplantation; Urology & Nephrology GA QJ047 UT WOS:A1995QJ04700033 PM 7753470 ER PT J AU AUSTIN, HA BOUMPAS, DT VAUGHAN, EM BALOW, JE AF AUSTIN, HA BOUMPAS, DT VAUGHAN, EM BALOW, JE TI HIGH-RISK FEATURES OF LUPUS NEPHRITIS - IMPORTANCE OF RACE AND CLINICAL AND HISTOLOGICAL FACTORS IN 166 PATIENTS SO NEPHROLOGY DIALYSIS TRANSPLANTATION LA English DT Article DE GLOMERULONEPHRITIS; KIDNEY DISEASES; PATHOLOGY; PROGNOSIS; SYSTEMIC LUPUS ERYTHEMATOSUS ID LONG-TERM PROGNOSIS; IDIOPATHIC MEMBRANOUS NEPHROPATHY; REQUIRING PROLONGED OBSERVATION; SYSTEMIC LUPUS; RENAL BIOPSY; MEMBRANOPROLIFERATIVE GLOMERULONEPHRITIS; MORPHOLOGICAL PARAMETERS; CONTROLLED TRIAL; FOLLOW-UP; ERYTHEMATOSUS AB Background. The pleomorphic nature of lupus nephritis has confounded efforts to refine estimates of prognosis. Consideration of interactions among prognostic factors may help to identify high-risk patients. Methods. By univariate and multivariate survival analysis, race and attributes of severe active lupus nephritis were evaluated as potentially important prognostic factors in 166 patients upon entry into prospective therapeutic trials of lupus kidney disease. Results. Black patients were significantly more likely than others to develop renal insufficiency. Cellular crescents emerged as the most predictive active pathological feature and interstitial fibrosis was the strongest chronic histological prognostic factor. Combinations of these morphological attributes identified particularly high-risk individuals. Patients with 50% or more cellular crescents and those with less extensive cellular crescents plus moderate to severe interstitial fibrosis were at markedly increased risk for doubling serum creatinine compared to those who lacked these histologic features (P < 0.0001). Azotaemia, anaemia, hypocomplementaemia, hypertension, tubular atrophy and glomerular sclerosis were also associated with an increased probability of renal function deterioration. Serum creatinine, haematocrit, race, and kidney pathology data emerged as independent predictors of renal insufficiency. Black patients in this study were more likely than the others to have high-risk histological features, including extensive cellular crescents (greater than or equal to 50%) and moderate to severe interstitial fibrosis, prior to randomization. Conclusions. Combinations of high-risk demographic, clinical and histological attributes identify patients at increased risk for progressive renal function deterioration. Several factors, including the severity of kidney biopsy findings, probably contribute to the poor prognosis of Black patients in this study population. C1 NIH,DEPT NURSING,CTR CLIN,BETHESDA,MD. RP AUSTIN, HA (reprint author), NIDDKD,KIDNEY DIS SECT,9000 ROCKVILLE PIKE,BLDG 10,ROOM 3N112,BETHESDA,MD 20892, USA. NR 70 TC 154 Z9 159 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0931-0509 J9 NEPHROL DIAL TRANSPL JI Nephrol. Dial. Transplant. PY 1995 VL 10 IS 9 BP 1620 EP 1628 PG 9 WC Transplantation; Urology & Nephrology SC Transplantation; Urology & Nephrology GA RV946 UT WOS:A1995RV94600033 PM 8559480 ER PT J AU SPANGLER, EL WAGGIE, KS REA, W ROBERTS, D HENGEMIHLE, J DANON, D INGRAM, DK AF SPANGLER, EL WAGGIE, KS REA, W ROBERTS, D HENGEMIHLE, J DANON, D INGRAM, DK TI RELATIONSHIP OF HEMATOLOGICAL VARIABLES TO LEARNING-PERFORMANCE IN AGED FISCHER-344 RATS SO NEUROBIOLOGY OF AGING LA English DT Article DE HEMATOLOGY; HEMATOCRIT; PATHOLOGY; ERYTHROCYTES; HEMOGLOBIN; IMMUNOLOGY; LEARNING; MORBIDITY ID GRANULAR LYMPHOCYTE LEUKEMIA; AGING RESEARCH; CELL AGE; MODELS; DONOR AB The relationship between hematological variables and the ability to perform behaviorally in two learning tests was evaluated in male F344 rats aged 22-24 months. Rats were screened for ability to meet criterion for learning one-way active avoidance in a straight runway task. Rats failing to meet criterion were given no further testing and were assigned to Group 1 (G1). Rats meeting criterion were tested in a I l-unit T-maze (2 days, 10 trials/day). Failure to negotiate the T-maze within 600 s on any three trials resulted in assignment to Group 2 (G2) with no further testing. Rats successfully completing both tasks constituted Group 3 (G3). Trunk blood was collected following behavioral testing and was assayed to determine red blood cell count (RBC), hematocrit (HCT), hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell count (WBC), bands (END), polymorphs (POLY), lymphocytes (LYM), monocytes (MON), and eosinophils (EOS). The combined G1/G2 group had significantly lower RBC, HCT, HGB, and EOS but significantly higher MCV and MCH than G3 rats. Correlational analysis revealed a positive relationship of group membership (i.e., learning test completion) to RBC, HCT, HGB, and EOS, but a negative correlation of group membership to MCH. No significant correlation emerged between any hematological characteristic and performance in either behavioral task. These results suggest that a simple blood test to determine HCT may be a useful screen for removal of moribund rats from aging studies attempting to control for effects of health on behavioral performance in rodent models. C1 NIA,GERONTOL RES CTR,CELLULAR & MOLEC BIOL LAB,MOLEC PHYSIOL & GENET SECT,BALTIMORE,MD 21224. JOHNS HOPKINS BAYVIEW MED CTR,CTR ASTHMA & ALLERGY,BALTIMORE,MD 21224. WEIZMANN INST SCI,IL-76100 REHOVOT,ISRAEL. NR 20 TC 9 Z9 9 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD JAN-FEB PY 1995 VL 16 IS 1 BP 85 EP 89 DI 10.1016/0197-4580(95)80011-F PG 5 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA QD448 UT WOS:A1995QD44800011 PM 7723940 ER PT B AU Golomb, D Wang, XJ Rinzel, J AF Golomb, D Wang, XJ Rinzel, J BE Bower, JM TI Partial and full synchrony of thalamic spindle oscillations SO NEUROBIOLOGY OF COMPUTATION: PROCEEDINGS OF THE THIRD ANNUAL COMPUTATION AND NEURAL SYSTEMS CONFERENCE LA English DT Proceedings Paper CT 3rd Annual Computation and Neural Systems Meeting (CNS 94) CY JUL 21-26, 1994 CL MONTEREY, CA SP NIMH, NIDOCD, NICHHD, NINDS, NASA, AMES Res Ctr, Moffett Field C1 NIDDK,MATH RES BRANCH,BETHESDA,MD 20814. NR 0 TC 1 Z9 1 U1 0 U2 1 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS BN 0-7923-9543-3 PY 1995 BP 215 EP 220 PG 6 WC Computer Science, Artificial Intelligence; Engineering, Biomedical; Neurosciences SC Computer Science; Engineering; Neurosciences & Neurology GA BE43J UT WOS:A1995BE43J00035 ER PT B AU WETSEL, WC AF WETSEL, WC BE Plant, TM Lee, PA TI Processing of the GnRH prohormone SO NEUROBIOLOGY OF PUBERTY SE ENDOCRINE UPDATES LA English DT Proceedings Paper CT 4th International Conference on the Control of the Onset of Puberty CY SEP 29-OCT 01, 1994 CL PITTSBURGH, PA SP Univ Pittsburgh Med Ctr, Univ Pittsburgh Sch Med, Magee Womens Hosp, Childrens Hosp Pittsburgh, TAP Pharm Inc, Roberts Labs Inc, Genentech Inc, Eli Lilly & Co, Bristol Myers Squibb Co, Kabi Pharmacia Inc, Serono Labs Inc C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,HORMONE ACT WORKGRP,RES TRIANGLE PK,NC 27709. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SOCIETY ENDOCRINOLOGY PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ BN 1-898099-06-5 J9 ENDOCRIN UPDAT PY 1995 BP 25 EP 39 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BD69L UT WOS:A1995BD69L00003 ER PT B AU CUTLER, GB AF CUTLER, GB BE Plant, TM Lee, PA TI Central puberty in disorders with accelerated peripheral maturation SO NEUROBIOLOGY OF PUBERTY SE ENDOCRINE UPDATES LA English DT Proceedings Paper CT 4th International Conference on the Control of the Onset of Puberty CY SEP 29-OCT 01, 1994 CL PITTSBURGH, PA SP Univ Pittsburgh Med Ctr, Univ Pittsburgh Sch Med, Magee Womens Hosp, Childrens Hosp Pittsburgh, TAP Pharm Inc, Roberts Labs Inc, Genentech Inc, Eli Lilly & Co, Bristol Myers Squibb Co, Kabi Pharmacia Inc, Serono Labs Inc C1 NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOCIETY ENDOCRINOLOGY PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ BN 1-898099-06-5 J9 ENDOCRIN UPDAT PY 1995 BP 209 EP 215 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA BD69L UT WOS:A1995BD69L00017 ER PT J AU DIVERSEPIERLUISSI, M GOLDSMITH, PK DUNLAP, K AF DIVERSEPIERLUISSI, M GOLDSMITH, PK DUNLAP, K TI TRANSMITTER-MEDIATED INHIBITION OF N-TYPE CALCIUM CHANNELS IN SENSORY NEURONS INVOLVES MULTIPLE GTP-BINDING PROTEINS AND SUBUNITS SO NEURON LA English DT Article ID RAT SYMPATHETIC NEURONS; ROOT GANGLION NEURONS; MUSCARINIC K+-CHANNEL; BETA-GAMMA-SUBUNITS; VOLTAGE DEPENDENCE; PERTUSSIS-TOXIN; PHOSPHOLIPASE-C; OMEGA-CONOTOXIN; CA2+ CHANNELS; KINASE-C AB The modulation of voltage-activated Ca2+ channels by neurotransmitters and peptides is very likely a primary means of regulating Ca2+-dependent physiological functions such as neurosecretion, muscle contraction, and membrane excitability. In neurons, N-type Ca2+ channels (defined as omega-conotoxin GVIA-sensitive) are one prominent target for transmitter-mediated inhibition. This inhibition is widely thought to result from a shift in the voltage dependence of channel gating. Recently, however, voltage-independent inhibition has also been described for N channels. As embryonic chick dorsal root ganglion neurons express both of these biophysically distinct modulatory pathways, we have utilized these cells to test the hypothesis that the voltage-dependent and -independent actions of transmitters are mediated by separate biochemical pathways. We have confirmed this hypothesis by demonstrating that the two modulatory mechanisms activated by a single transmitter involve not only different classes of G protein but also different G protein subunits. C1 TUFTS UNIV,SCH MED,DEPT NEUROSCI,BOSTON,MA 02111. NIDDKD,METAB DIS BRANCH,BETHESDA,MD 20892. RP DIVERSEPIERLUISSI, M (reprint author), TUFTS UNIV,SCH MED,DEPT PHYSIOL,BOSTON,MA 02111, USA. FU NINDS NIH HHS [NS16483] NR 60 TC 117 Z9 119 U1 0 U2 3 PU CELL PRESS PI CAMBRIDGE PA 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0896-6273 J9 NEURON JI Neuron PD JAN PY 1995 VL 14 IS 1 BP 191 EP 200 DI 10.1016/0896-6273(95)90254-6 PG 10 WC Neurosciences SC Neurosciences & Neurology GA QC548 UT WOS:A1995QC54800020 PM 7826637 ER PT S AU Ruda, MA Ren, K Besse, D AF Ruda, MA Ren, K Besse, D BE Nyberg, F Sharma, HS WiesenfeldHallin, Z TI Regulation of spinal neuropeptide genes in a rat model of peripheral inflammation and hyperalgesia SO NEUROPEPTIDES IN THE SPINAL CORD SE Progress in Brain Research LA English DT Review ID SUPERFICIAL DORSAL HORN; PRIMARY AFFERENT-FIBERS; IMMEDIATE-EARLY GENES; PEPTIDE IMMUNOREACTIVE AXONS; NEONATAL CAPSAICIN TREATMENT; FOS-LIKE IMMUNOREACTIVITY; MULTIPLE OPIOID SYSTEMS; TAIL-FLICK REFLEX; SUBSTANCE-P; C-FOS RP Ruda, MA (reprint author), NIDR, NIH,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 49, RM 1A-11, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA. NR 107 TC 7 Z9 7 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 BN 0-444-81719-0 J9 PROG BRAIN RES JI Prog. Brain Res. PY 1995 VL 104 BP 349 EP 365 PG 17 WC Biochemistry & Molecular Biology; Neurosciences; Physiology SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Physiology GA BE85G UT WOS:A1995BE85G00020 PM 8552779 ER PT S AU Herning, RI Guo, XY Lange, WR AF Herning, RI Guo, XY Lange, WR BE Trembly, B Slikker, W TI The effects of nimodipine on the EEG of substance abusers SO NEUROPROTECTIVE AGENTS: CLINICAL AND EXPERIMENTAL ASPECTS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 2nd International Conference on Neuroprotective Agents - Clinical and Experimental Aspects CY JUL 31-AUG 03, 1994 CL LAKE GEORGE, NY SP FDA, Natl Ctr Toxicol Res, Jefferson, Arkansas, Dept Vet Affairs Med Ctr, Togus, Maine ID COCAINE USE; PERFUSION C1 NIDA,ADDICT RES CTR,INTRAMURAL RES PROGRAM,MOLEC NEUROPSYCHIAT SECT,MED AFFAIRS BRANCH,BALTIMORE,MD 21224. RP Herning, RI (reprint author), NIDA,ADDICT RES CTR,INTRAMURAL RES PROGRAM,MOLEC NEUROPSYCHIAT SECT,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 23 TC 6 Z9 7 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-945-2 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 765 BP 143 EP 151 DI 10.1111/j.1749-6632.1995.tb16570.x PG 9 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA BE39D UT WOS:A1995BE39D00013 PM 7486602 ER PT S AU Herning, RI Guo, XY Lange, WR AF Herning, RI Guo, XY Lange, WR BE Trembly, B Slikker, W TI Nimodipine improves information processing in substance abusers SO NEUROPROTECTIVE AGENTS: CLINICAL AND EXPERIMENTAL ASPECTS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 2nd International Conference on Neuroprotective Agents - Clinical and Experimental Aspects CY JUL 31-AUG 03, 1994 CL LAKE GEORGE, NY SP FDA, Natl Ctr Toxicol Res, Jefferson, Arkansas, Dept Vet Affairs Med Ctr, Togus, Maine ID EVENT-RELATED POTENTIALS; METHYLPHENIDATE; PERFORMANCE; VIGILANCE; MEMORY; P300 C1 NIDA,ADDICT RES CTR,MOLEC NEUROPSYCHIAT SECT,IRP,MED AFFAIRS BRANCH,BALTIMORE,MD 21224. RP Herning, RI (reprint author), NIDA,ADDICT RES CTR,MOLEC NEUROPSYCHIAT SECT,IRP,NEUROSCI BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 31 TC 5 Z9 9 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-945-2 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 765 BP 152 EP 159 DI 10.1111/j.1749-6632.1995.tb16571.x PG 8 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA BE39D UT WOS:A1995BE39D00014 PM 7486603 ER PT S AU vonLubitz, DKJE Carter, MF Beenhakker, M Lin, RCS Jacobson, KA AF vonLubitz, DKJE Carter, MF Beenhakker, M Lin, RCS Jacobson, KA BE Trembly, B Slikker, W TI Adenosine: A prototherapeutic concept in neurodegeneration SO NEUROPROTECTIVE AGENTS: CLINICAL AND EXPERIMENTAL ASPECTS SE Annals of the New York Academy of Sciences LA English DT Review CT 2nd International Conference on Neuroprotective Agents - Clinical and Experimental Aspects CY JUL 31-AUG 03, 1994 CL LAKE GEORGE, NY SP FDA, Natl Ctr Toxicol Res, Jefferson, Arkansas, Dept Vet Affairs Med Ctr, Togus, Maine ID RAT CEREBRAL-CORTEX; ADENYLATE CYCLASE SYSTEM; AMINO-ACID RELEASE; METHYL-D-ASPARTATE; RECEPTOR AGONIST; SYNAPTIC TRANSMISSION; ENDOGENOUS ADENOSINE; HIPPOCAMPUS INVITRO; A1 RECEPTORS; CYCLIC-AMP C1 HAHNEMANN UNIV, DEPT PHYSIOL & BIOPHYS, PHILADELPHIA, PA 19102 USA. RP vonLubitz, DKJE (reprint author), NIDDKD, BIOORGAN CHEM LAB, MOLEC RECOGNIT SECT, BETHESDA, MD 20892 USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031117-20] NR 124 TC 29 Z9 30 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-946-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 765 BP 163 EP 178 PG 16 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA BE39D UT WOS:A1995BE39D00015 PM 7486604 ER PT B AU Spatz, M Stanimirovic, D McCarron, RM AF Spatz, M Stanimirovic, D McCarron, RM BE Greenwood, J Begley, DJ Segal, MB TI Endothelin as a mediator of blood-brain barrier function SO NEW CONCEPTS OF A BLOOD-BRAIN BARRIER LA English DT Proceedings Paper CT Symposium, in Honor of Professor Michael Bradbury, on New Concepts of a Blood-Brain Barrier CY JUL 04-06, 1994 CL LONDON, ENGLAND SP Kings Coll London, Biomed Sci Div, Allergan Ltd UK, Glaxo Ltd UK, Zeneca Ltd UK C1 NINCDS,STROKE BRANCH,NIH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45204-9 PY 1995 BP 47 EP 61 PG 15 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BE85Z UT WOS:A1995BE85Z00005 ER PT B AU Rapoport, SI Robinson, PJ AF Rapoport, SI Robinson, PJ BE Greenwood, J Begley, DJ Segal, MB TI Long-chain fatty acid transport at the blood-brain barrier and incorporation into brain phospholipids: A new in vivo method for examining neuroplasticity, and brain second messenger systems involving phospholipase A(2) activation SO NEW CONCEPTS OF A BLOOD-BRAIN BARRIER LA English DT Proceedings Paper CT Symposium, in Honor of Professor Michael Bradbury, on New Concepts of a Blood-Brain Barrier CY JUL 04-06, 1994 CL LONDON, ENGLAND SP Kings Coll London, Biomed Sci Div, Allergan Ltd UK, Glaxo Ltd UK, Zeneca Ltd UK C1 NIA,NEUROSCI LAB,NIH,BETHESDA,MD 20892. NR 0 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45204-9 PY 1995 BP 119 EP 140 PG 22 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BE85Z UT WOS:A1995BE85Z00013 ER PT B AU Greig, NH Brossi, A Pei, XF Ingram, DK Soncrant, TT AF Greig, NH Brossi, A Pei, XF Ingram, DK Soncrant, TT BE Greenwood, J Begley, DJ Segal, MB TI Designing drugs for optimal nervous system activity SO NEW CONCEPTS OF A BLOOD-BRAIN BARRIER LA English DT Proceedings Paper CT Symposium, in Honor of Professor Michael Bradbury, on New Concepts of a Blood-Brain Barrier CY JUL 04-06, 1994 CL LONDON, ENGLAND SP Kings Coll London, Biomed Sci Div, Allergan Ltd UK, Glaxo Ltd UK, Zeneca Ltd UK C1 NIA,NIH,NEUROSCI LAB,BETHESDA,MD 20892. NR 0 TC 16 Z9 17 U1 0 U2 1 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45204-9 PY 1995 BP 251 EP 264 PG 14 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BE85Z UT WOS:A1995BE85Z00025 ER PT B AU Smith, QR AF Smith, QR BE Greenwood, J Begley, DJ Segal, MB TI Carrier-mediated drug transport at the blood-brain barrier and the potential for drug targeting to the brain SO NEW CONCEPTS OF A BLOOD-BRAIN BARRIER LA English DT Proceedings Paper CT Symposium, in Honor of Professor Michael Bradbury, on New Concepts of a Blood-Brain Barrier CY JUL 04-06, 1994 CL LONDON, ENGLAND SP Kings Coll London, Biomed Sci Div, Allergan Ltd UK, Glaxo Ltd UK, Zeneca Ltd UK C1 NIA,NIH,NEUROSCI LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45204-9 PY 1995 BP 265 EP 276 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BE85Z UT WOS:A1995BE85Z00026 ER PT B AU Ishihara, S Tsubaki, SI Chang, L Brightman, MW AF Ishihara, S Tsubaki, SI Chang, L Brightman, MW BE Greenwood, J Begley, DJ Segal, MB TI Peripheral tissue grafts as portals into brain for solutes and leukocytes SO NEW CONCEPTS OF A BLOOD-BRAIN BARRIER LA English DT Proceedings Paper CT Symposium, in Honor of Professor Michael Bradbury, on New Concepts of a Blood-Brain Barrier CY JUL 04-06, 1994 CL LONDON, ENGLAND SP Kings Coll London, Biomed Sci Div, Allergan Ltd UK, Glaxo Ltd UK, Zeneca Ltd UK C1 NINCDS,NIH,NEUROBIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45204-9 PY 1995 BP 287 EP 298 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Physiology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Physiology GA BE85Z UT WOS:A1995BE85Z00028 ER PT S AU MICHEJDA, CJ AF MICHEJDA, CJ BE Zeller, WJ DIncalci, M Newell, DR TI Quantum mechanical calculations as aids in drug design SO NOVEL APPROACHES IN ANTICANCER DRUG DESIGN: MOLECULAR MODELLING - NEW TREATMENT STRATEGIES SE CONTRIBUTIONS TO ONCOLOGY LA English DT Proceedings Paper CT International Symposium on Novel Approaches in Cancer Therapy CY DEC 01-04, 1993 CL HEIDELBERG, GERMANY SP German Canc Res Ctr, EORTC Grp, German Canc Res Ctr, PAMM Grp, German Canc Res Ctr, SPG Grp, German Canc Soc C1 NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MACROMOLEC STRUCT LAB,FREDERICK,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0250-3220 BN 3-8055-6043-5 J9 CONTR ONCOL PY 1995 VL 49 BP 1 EP 9 PG 9 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA BD68L UT WOS:A1995BD68L00001 ER PT J AU KAWAI, K CHANNING, MA KIESEWETTER, DO ECKELMAN, WC AF KAWAI, K CHANNING, MA KIESEWETTER, DO ECKELMAN, WC TI SYNTHESIS OF POLYMER-BOUND 6-THIOLATOMERCURY AND 6-MERCURIC SULFONATE DOPA PRECURSORS AND THEIR HALODEMERCURATION REACTIVITY SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article ID 6-FLUORO-L-DOPA AB Fluorodemercuration has the greatest utility for the preparation of 6-[F-18]DOPA, but requires separation from unreacted mercury precursor and other mercury-containing compounds. One approach is the development of a polymer-bound mercury precursor. Tn this study, polymer-bound 6-thiolatomercury and 6-mercuric sulfonate DOPA derivatives, and its monomeric analogs were synthesized. Fluorodemercuration of monomeric analog of mercuric sulfonate gave half the yield (14-15%) while iododemercuration gave the same yield (38%) compared with a 6-mercuric trifluoroacetate protected DOPA. The mercuric sulfonate undergoes halodemercuration, so polymer-bound halodemercuration precursors may be useful as precursors of 6-[F-18]DOPA. C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT PET,BETHESDA,MD 20892. RP KAWAI, K (reprint author), SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT RADIOPHARMACEUT CHEM,SHINJUKU KU,TOKYO 162,JAPAN. NR 11 TC 5 Z9 5 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0883-2897 J9 NUCL MED BIOL JI Nucl. Med. Biol. PD JAN PY 1995 VL 22 IS 1 BP 37 EP 43 DI 10.1016/0969-8051(94)00079-Y PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA QJ044 UT WOS:A1995QJ04400006 PM 7735168 ER PT J AU MARQUEZ, VE JEONG, LS NICKLAUS, MC GEORGE, C AF MARQUEZ, VE JEONG, LS NICKLAUS, MC GEORGE, C TI SYNTHESIS AND BIOLOGICAL-ACTIVITY OF SUGAR-FLUORINATED 2',3'-DIDEOXY-4'-THIORIBOFURANOSYL NUCLEOSIDES SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT 11th International Round Table on Nucleosides, Nucleotides and Their Biological Applications CY SEP 07-11, 1994 CL KATHOLIEK UNIV LEUVEN, LEUVEN, BELGIUM HO KATHOLIEK UNIV LEUVEN ID ANALOGS AB The efficient DAST fluorination of deoxy-4'-thiopyrimidine nucleosides is reported. The cytidine analogue 3b was marginally effective against HIV. C1 USN,RES LAB,STRUCT MATTER LAB,WASHINGTON,DC 20375. RP MARQUEZ, VE (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MED CHEM LAB,BETHESDA,MD 20892, USA. RI Nicklaus, Marc/N-4183-2014 NR 13 TC 6 Z9 6 U1 0 U2 5 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1995 VL 14 IS 3-5 BP 555 EP 558 DI 10.1080/15257779508012426 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA RB956 UT WOS:A1995RB95600067 ER PT J AU TORRENCE, PF XIAO, W LI, GY KHAMNEI, S LESIAK, K MARAN, A MAITRA, R KUMAR, A DONG, B WILLIAMS, BRG SILVERMAN, RH AF TORRENCE, PF XIAO, W LI, GY KHAMNEI, S LESIAK, K MARAN, A MAITRA, R KUMAR, A DONG, B WILLIAMS, BRG SILVERMAN, RH TI TARGETING RNA FOR DEGRADATION WITH A (2',5')-OLIGOADENYLATE ANTISENSE CHIMERA SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article; Proceedings Paper CT 11th International Round Table on Nucleosides, Nucleotides and Their Biological Applications CY SEP 07-11, 1994 CL KATHOLIEK UNIV LEUVEN, LEUVEN, BELGIUM HO KATHOLIEK UNIV LEUVEN ID DEPENDENT PROTEIN-KINASE; INTERFERON AB A novel method is described to Selectively cleave RNA by harnessing the 2-5A-dependent ribonuclease. C1 CLEVELAND CLIN FDN,DEPT CANC BIOL,CLEVELAND,OH 44195. RP TORRENCE, PF (reprint author), NIDDKD,BIOMED CHEM SECT,MED CHEM LAB,BETHESDA,MD 20892, USA. RI Williams, Bryan/A-5021-2009 OI Williams, Bryan/0000-0002-4969-1151 NR 7 TC 1 Z9 1 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1995 VL 14 IS 3-5 BP 1073 EP 1076 DI 10.1080/15257779508012537 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA RB956 UT WOS:A1995RB95600178 ER PT J AU KOVACS, T VANAERSCHOT, A HERDEWIJN, P TORRENCE, PF AF KOVACS, T VANAERSCHOT, A HERDEWIJN, P TORRENCE, PF TI SOLID-PHASE SYNTHESIS OF 2',5'-OLIGOADENYLATES CONTAINING 3'-FLUORINATED RIBOSE SO NUCLEOSIDES & NUCLEOTIDES LA English DT Article ID OLIGONUCLEOTIDE SYNTHESIS; CHEMICAL SYNTHESIS; OLIGORIBONUCLEOTIDES; OLIGODEOXYRIBONUCLEOTIDES; PHOSPHORAMIDITE; NUCLEOSIDES; BINDING; SUPPORT; 2-5A; BASE AB 2',5'-phosphodiester bond-linked oligoadenylate trimers with 3'-fluoro-3'-deoxyadenosine residues incorporated at specific positions of the nucleotide sequence were synthesized by the solid phase phosphite triester (phosphoramidite) method. The syntheses were in the 2' to 5' direction and were performed manually using commercially available microcolumns. The oligonucleotides were 5'-end phosphorylated on the support before deprotection. C1 NIDDK,MED CHEM LAB,BIOL CHEM SECT,BETHESDA,MD 20892. CATHOLIC UNIV LEUVEN,REGA INST MED RES,MED CHEM LAB,B-3000 LOUVAIN,BELGIUM. NR 39 TC 2 Z9 2 U1 0 U2 2 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0732-8311 J9 NUCLEOS NUCLEOT JI Nucleosides Nucleotides PY 1995 VL 14 IS 6 BP 1259 EP 1267 DI 10.1080/15257779508010689 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA RP796 UT WOS:A1995RP79600009 ER PT J AU HURD, SS AF HURD, SS TI RX FOR A HEALTHY NINR SO NURSING RESEARCH LA English DT Editorial Material RP HURD, SS (reprint author), NINR,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER J NURSING CO PI NEW YORK PA 555 W 57TH ST, NEW YORK, NY 10019-2961 SN 0044-7781 J9 NURS RES JI Nurs. Res. PD JAN-FEB PY 1995 VL 44 IS 1 BP 3 EP 3 DI 10.1097/00006199-199501000-00001 PG 1 WC Nursing SC Nursing GA RU600 UT WOS:A1995RU60000001 PM 7862542 ER PT S AU ADAMSON, RH THORGEIRSSON, UP AF ADAMSON, RH THORGEIRSSON, UP BE Longenecker, JB Kritchevsky, D Drezner, MK TI CARCINOGENS IN FOODS - HETEROCYCLIC AMINES AND CANCER AND HEART DISEASE SO NUTRITION AND BIOTECHNOLOGY IN HEART DISEASE AND CANCER SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT Conference on Nutrition and Biotechnology in Heart Disease and Cancer CY DEC 05-07, 1993 CL RESEARCH TRIANGLE PARK, NC SP UNIV N CAROLINA, INST NUTR, DUKE UNIV MED CTR, SARAH W STEDMAN CTR, N CAROLINA BIOTECHNOL CTR C1 NCI,DIV CANC ETIOL,BETHESDA,MD 20892. NR 0 TC 11 Z9 13 U1 0 U2 1 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-44994-3 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 369 BP 211 EP 220 PG 10 WC Biochemistry & Molecular Biology; Oncology; Cardiac & Cardiovascular Systems; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Oncology; Cardiovascular System & Cardiology; Nutrition & Dietetics GA BC72K UT WOS:A1995BC72K00020 PM 7598010 ER PT S AU GREENWALD, P CLIFFORD, C PILCH, S HEIMENDINGER, J KELLOFF, G AF GREENWALD, P CLIFFORD, C PILCH, S HEIMENDINGER, J KELLOFF, G BE Longenecker, JB Kritchevsky, D Drezner, MK TI NEW DIRECTIONS IN DIETARY STUDIES IN CANCER - THE NATIONAL-CANCER-INSTITUTE SO NUTRITION AND BIOTECHNOLOGY IN HEART DISEASE AND CANCER SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT Conference on Nutrition and Biotechnology in Heart Disease and Cancer CY DEC 05-07, 1993 CL RESEARCH TRIANGLE PARK, NC SP UNIV N CAROLINA, INST NUTR, DUKE UNIV MED CTR, SARAH W STEDMAN CTR, N CAROLINA BIOTECHNOL CTR C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. RI Pilch, Susan/A-8015-2010 NR 0 TC 5 Z9 5 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-44994-3 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 369 BP 229 EP 239 PG 11 WC Biochemistry & Molecular Biology; Oncology; Cardiac & Cardiovascular Systems; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Oncology; Cardiovascular System & Cardiology; Nutrition & Dietetics GA BC72K UT WOS:A1995BC72K00022 PM 7598012 ER PT J AU SCHWARTZ, JL WEST, K SHKLAR, DP SHKLAR, G AF SCHWARTZ, JL WEST, K SHKLAR, DP SHKLAR, G TI ALTERED CYTOKERATIN EXPRESSION IN CARCINOGENESIS INHIBITION BY ANTIOXIDANT NUTRIENTS SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID KERATIN EXPRESSION; EPIDERMAL DIFFERENTIATION; TERMINAL DIFFERENTIATION; ORAL EPITHELIUM; PATTERNS; CARCINOMAS; SUBTYPES; CELLS AB Epidermoid carcinomas were induced in hamster buccal pouches with use of 7,12 dimethylbenz[a]anthracene (DMBA). In five animals that served as tumor controls (Group 1), right buccal pouches were painted with DMBA (0.5% solution in mineral oil) thrice weekly for 14 weeks. In Jive animals (Group 2), right buccal pouches were painted with DMBA and reduced glutathione (GSH) was administered systemically by mouth. Five animals (Group 3) received vitamin E instead of glutathione. An additional 20 animals (Groups 4, 5, 6, and 7) were untreated, vehicle, glutathione, and vitamin E controls, respectively. Glutathione and vitamin E were given in doses of 10 mg/kg in 0.5 ml of mineral oil thrice weekly on days alternate to DMBA painting. Treatment by GSH and vitamin E reduced the number and size of tumors that were formed. Histopathologically, there were also fewer sites of dysplasia, carcinoma in situ, and early invasive epidermoid carcinoma than in the tumor control animals. The formalin-fixed and parafin-embedded buccal pouch sections were stained immunohistochemically with use of monoclonal antibodies for cytokeratins. These included high-molecular-weight keratins (50,000-68,000 mol wt) 10, 13, and 8 (k10, k13, and k8, respectively). Oral carcinomas and dysplastic sites exhibited basal and suprabasal (spinous layer) high levels of k10, k13, and k8 staining. Treatment with GSH or vitamin E increased the suprabasal staining for high-molecular-weight keratins and reduced the protein expression for k10, k13, or k8. This pattern of staining was observed in dysplastic as well as in carcinoma sites. These results indicate that cytokeratin protein expression could contribute to a common biomarker analysis for chemoprevention. C1 HARVARD UNIV,SCH DENT MED,DEPT ORAL MED & DIAGNOST SCI,DIV ORAL PATHOL,BOSTON,MA 02115. TUFTS UNIV,SCH DENT MED,BOSTON,MA 02115. RP SCHWARTZ, JL (reprint author), NIDR,DIV MOLEC EPIDEMIOL,ORAL DIS & PREVENT PROGRAM,BETHESDA,MD 20892, USA. NR 18 TC 15 Z9 15 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer-Int. J. PY 1995 VL 24 IS 1 BP 47 EP 56 PG 10 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA RH693 UT WOS:A1995RH69300005 PM 7491297 ER PT J AU PATTERSON, BH HARLAN, LC BLOCK, G KAHLE, L AF PATTERSON, BH HARLAN, LC BLOCK, G KAHLE, L TI FOOD CHOICES OF WHITES, BLACKS, AND HISPANICS - DATA FROM THE 1987 NATIONAL-HEALTH INTERVIEW SURVEY SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID FREQUENCY QUESTIONNAIRE; EPIDEMIOLOGIC EVIDENCE; CANCER; VEGETABLES; DIET; CAROTENOIDS; VALIDATION; RECORDS; FRUIT AB Dietary guidelines posit an association between diet and cancer. Different cancer mortality races among whites, blacks, and Hispanics may be related to differences in diet. Food frequency data from the 1987 National Health Interview, Survey on 20,143 adults were used to estimate the percentage of adults, by gender and race/ethnicity, who consume some 59 foods six or more times per year, median number of servings for consumers, and frequency of consumption of skin on poultry and fat on red meat. On the basis of percent consumption of these foods, women appear to have a more diverse diet than men. Women eat more fruits and vegetables, less meat, and fewer high-fat foods and drink fewer alcoholic beverages. Whites eat a more varied diet than blacks and Hispanics; blacks eat more fried and high-fat food; consumption of high-fat foods is lowest among Hispanics. Public health messages, especially those aimed at cancer prevention, should be targeted at increasing the overall consumption of fruits and vegetables, decreasing consumption of high-fat foods, especially among white and black men, and increasing consumption of those healthful foods already consumed by particular race/ethnicity groups. C1 UNIV CALIF BERKELEY,BERKELEY,CA 94720. INFORMAT MANAGEMENT SERV INC,SILVER SPRING,MD 20910. RP PATTERSON, BH (reprint author), NCI,DIV CANC PREVENT & CONTROL,BIOMETRY BRANCH,EXECUT PLAZA N,RM 344,BETHESDA,MD 20892, USA. RI Block, Gladys/E-3304-2010 NR 29 TC 74 Z9 74 U1 0 U2 4 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 0163-5581 J9 NUTR CANCER JI Nutr. Cancer-Int. J. PY 1995 VL 23 IS 2 BP 105 EP 119 PG 15 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA QN100 UT WOS:A1995QN10000001 PM 7644380 ER PT J AU LANDON, MB MCNELLIS, D HARGER, J MERCER, B THOM, E AF LANDON, MB MCNELLIS, D HARGER, J MERCER, B THOM, E TI PREVENTION OF NEONATAL GROUP-B STREPTOCOCCAL INFECTION - REPLY SO OBSTETRICS AND GYNECOLOGY LA English DT Letter ID DISEASE RP LANDON, MB (reprint author), NICHHD,BETHESDA,MD 20892, USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD JAN PY 1995 VL 85 IS 1 BP 160 EP 161 PG 2 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA PX706 UT WOS:A1995PX70600040 ER PT J AU ABIOSE, AO AWADZI, K CUPP, EW DUKE, BOL GEMADE, EII OTTESEN, EA PETRALANDA, I ROUNGOU, JB SCHULZKEY, H WEISS, N BALDRY, DAT DADZIE, KY RAMACHANDRAN, CP REMME, JHF AF ABIOSE, AO AWADZI, K CUPP, EW DUKE, BOL GEMADE, EII OTTESEN, EA PETRALANDA, I ROUNGOU, JB SCHULZKEY, H WEISS, N BALDRY, DAT DADZIE, KY RAMACHANDRAN, CP REMME, JHF TI REPORT OF A WHO EXPERT COMMITTEE ON ONCHOCERCIASIS CONTROL - INTRODUCTION SO ONCHOCERCIASIS AND ITS CONTROL SE WHO TECHNICAL REPORT SERIES LA English DT Article ID WEST-AFRICA; CONTROL PROGRAM; VOLVULUS; IVERMECTIN; MICROFILARIAE; SIMULIUM; LARVICIDES; GUATEMALA; SKIN; AREA AB Although control of onchocerciasis by the Onchocerciasis Control Programme in West: Africa (OCP) has been highly successful, the disease remains endemic in 34 countries, affecting over 17 million people in tropical Africa, Latin America and the Arabian peninsula. It continues to be a major cause of blindness, severe eye lesions and disfiguring skin disease and has a considerable social and economic impact, in particular in the sub-Saharan savanna outside the OCP area. In recent years, however, the advent of ivermectin, a safe and effective microfilaricide, has raised hopes of eliminating onchocerciasis as a public health problem through both morbidity control and interruption of transmission in the endemic zones. This report of an international group of experts reviews both the disease and its control, identifying priorities for international and national control efforts and for future research. The report summarizes current knowledge of the filarial parasite Onchocerca volvulus, the blackfly vectors of onchocerciasis and the disease itself, including its clinical and epidemiological features, its socioeconomic consequences and the methods available for diagnosis, surveillance and treatment. The two main strategies for onchocerciasis control - chemotherapy with ivermectin and vector suppression through insecticide application and environmental management - are discussed in detail, together with training requirements for those participating in integrated control activities. Special emphasis is placed on the need for sustainable programmes for ivermectin distribution and for monitoring and evaluation, including epidemiological surveillance, if the prevalence of onchocerciasis is to be substantially reduced in endemic areas and the most efficient use made of available resources. C1 HOHOE HOSP,ONCHOCERCIASIS CHEMOTHERAPY RES CTR,HOHOE,GHANA. UNIV ARIZONA,TUCSON,AZ. RIVER BLINDNESS FDN,LANCASTER,ENGLAND. NATL ONCHOCERCIASIS CONTROL PROGRAMME,KADUNA,NIGERIA. NIAID,PARASIT DIS LAB,CLIN PARASITOL SECT,BETHESDA,MD 20892. AMAZON CTR INVEST & CONTROL TROP DIS,PUERTO AYACUCHO,VENEZUELA. PREVENT MED & CONTROL MAJOR ENDEM DIS,BANGUI,CENT AFR REPUBL. UNIV TUBINGEN,INST TROP MED,TUBINGEN,GERMANY. SWISS TROP INST,DEPT MED PARASITOL,CH-4002 BASEL,SWITZERLAND. CESSY,GEX,FRANCE. WHO,PROGRAMME PREVENT BLINDNESS,CH-1211 GENEVA,SWITZERLAND. WHO,DIV CONTROL TROP DIS,CH-1211 GENEVA,SWITZERLAND. WHO,SPECIAL PROGRAMME RES & TRAINING TROP DIS,WORLD BANK,UNDP,CH-1211 GENEVA,SWITZERLAND. RP ABIOSE, AO (reprint author), NATL EYE CTR,KADUNA,NIGERIA. NR 50 TC 62 Z9 64 U1 2 U2 2 PU WORLD HEALTH ORGANIZATION PI GENEVA PA 1211 27 GENEVA, SWITZERLAND SN 0512-3054 J9 WHO TECH REP SER PY 1995 VL 852 BP 1 EP & PG 0 WC Parasitology SC Parasitology GA BD89K UT WOS:A1995BD89K00001 ER PT J AU DLUGOSZ, AA GLICK, AB TENNENBAUM, T WEINBERG, WC YUSPA, SH AF DLUGOSZ, AA GLICK, AB TENNENBAUM, T WEINBERG, WC YUSPA, SH TI ISOLATION AND UTILIZATION OF EPIDERMAL-KERATINOCYTES FOR ONCOGENE RESEARCH SO ONCOGENE TECHNIQUES SE METHODS IN ENZYMOLOGY LA English DT Review ID HUMAN PAPILLOMAVIRUS TYPE-18; MOUSE SKIN CARCINOGENESIS; HA-RAS-ONCOGENE; TRANSFORMING GROWTH FACTOR-BETA-1; HARVEY SARCOMA-VIRUS; EPSTEIN-BARR-VIRUS; V-FOS ONCOGENE; NEOPLASTIC TRANSFORMATION; CELL-LINES; TERMINAL DIFFERENTIATION RP DLUGOSZ, AA (reprint author), NCI,CELLULAR CARCINOGENESIS & TUMOR PROMOT LAB,BLDG 37,BETHESDA,MD 20892, USA. RI Weinberg, Wendy/A-8920-2009 NR 105 TC 112 Z9 112 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 254 BP 3 EP 20 PG 18 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08E UT WOS:A1995BE08E00001 PM 8531694 ER PT J AU MIKI, T AARONSON, SA AF MIKI, T AARONSON, SA TI ISOLATION OF ONCOGENES BY EXPRESSION CDNA CLONING SO ONCOGENE TECHNIQUES SE METHODS IN ENZYMOLOGY LA English DT Review ID SYSTEM; INSERTS C1 CUNY,MT SINAI MED CTR,DERALD H RUTTENBERG CANC CTR,NEW YORK,NY 10029. RP MIKI, T (reprint author), NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,BETHESDA,MD 20892, USA. NR 10 TC 10 Z9 10 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 254 BP 196 EP 206 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08E UT WOS:A1995BE08E00013 PM 8531686 ER PT J AU TESSAROLLO, L PARADA, LF AF TESSAROLLO, L PARADA, LF TI IN-SITU HYBRIDIZATION SO ONCOGENE TECHNIQUES SE METHODS IN ENZYMOLOGY LA English DT Review ID INSITU HYBRIDIZATION; MOUSE DEVELOPMENT; GENE-EXPRESSION; RECEPTOR; EMBRYO RP TESSAROLLO, L (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MOLEC EMBRYOL SECT,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702, USA. RI Parada, luis/B-9400-2014 FU NCI NIH HHS [N01-CO-74101] NR 10 TC 21 Z9 21 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 254 BP 419 EP 430 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08E UT WOS:A1995BE08E00026 PM 8531703 ER PT J AU MATTEN, WT VANDEWOUDE, GF AF MATTEN, WT VANDEWOUDE, GF TI MICROINJECTION INTO XENOPUS OOCYTES SO ONCOGENE TECHNIQUES SE METHODS IN ENZYMOLOGY LA English DT Review ID MESSENGER-RNAS; CELL-CYCLE; MATURATION; INDUCTION; OOGENESIS; PROTEIN RP MATTEN, WT (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 21 TC 7 Z9 7 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 254 BP 458 EP 466 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08E UT WOS:A1995BE08E00029 PM 8531706 ER PT J AU KAPOOR, R SLADE, DL FUJIMORI, A POMMIER, Y HARKER, WG AF KAPOOR, R SLADE, DL FUJIMORI, A POMMIER, Y HARKER, WG TI ALTERED TOPOISOMERASE-I EXPRESSION IN 2 SUBCLONES OF HUMAN CEM LEUKEMIA SELECTED FOR RESISTANCE TO CAMPTOTHECIN SO ONCOLOGY RESEARCH LA English DT Article DE TOPOISOMERASE I; DRUG RESISTANCE; ACUTE LEUKEMIA; CAMPTOTHECIN; CELL CYTOTOXICITY ID TUMOR-CELL LINES; KINETOPLAST DNA NETWORKS; HAMSTER DC3F CELLS; CRITHIDIA-FASCICULATA; MULTIDRUG RESISTANCE; DRUG CAMPTOTHECIN; CLEAVAGE ACTIVITY; CYTO-TOXICITY; ACTINOMYCIN-D; IDENTIFICATION AB Two camptothecin-resistant variants of the CEM human leukemia cell line were developed by stepwise selection in camptothecin (CPT) in vitro. The two lines, named CEM/C1 and CEM/C2, were found to be approximately 31- and 970-fold less sensitive to CPT, respectively, than the CEM parental line and variably cross-resistant to the CPT analogs 9-amino-CPT, 10,11-methylenedioxy-CPT, and topotecan. Levels of DNA-protein complex formation resulting from cell exposure to CPT were found to be progressively reduced in the CPT-resistant cells, despite equivalent CPT accumulation in the drug-sensitive and -resistant cells. Nuclear extracts (1.0 M NaCl) prepared from the CEM/C1 and CEM/C2 lines contained 1.5- to a-fold less DNA topoisomerase I catalytic activity per microgram of protein than did extracts from the drug-sensitive CEM line, in association with altered sensitivity of the enzyme in the CEM/C1 and CEM/C2 extracts to the inhibitory activity of CPT. Only minor differences were noted in the CPT IC(50)s for the topoisomerase I activity in extracts from the two CPT-resistant cell lines, however, despite the marked differences in cellular sensitivity to CPT: There were notable differences in the level of CPT-induced cleavage of DNA oligonucleotides by topoisomerase I in nuclear extracts from CEM cells compared with the drug-resistant cell extracts, with very little oligonucleotide cleavage induced by enzyme in either drug-resistant cell type, despite the use of very high (100 mu M) CPT concentrations. The alterations in topoisomerase I catalytic activity were associated with reduced cellular levels of both immunoreactive topoisomerase I protein (representing 59 +/- 19% [CEM/C1] and 49 +/- 12% [CEM/C2] of that in GEM, respectively) and mRNA. In contrast, nuclear and whole cell levels of DNA topoisomerase II catalytic activity and protein were identical in the drug-sensitive and -resistant cell lines. These results suggest that the relatively low levels of CPT resistance displayed by the CEM/C1 cell line might have resulted from the alterations in DNA topoisomerase I activity and protein content found in those cells and imply that additional factors, such as qualitative changes in the topisomerase I enzyme, might be responsible for the higher levels of CPT resistance displayed in the CEM/C2 cells. C1 VET ADM MED CTR,DEPT MED,SALT LAKE CITY,UT 84148. UNIV UTAH,SCH MED,SALT LAKE CITY,UT 84148. NCI,DIV CANC TREATMENT,MOLEC PHARMACOL LAB,DEV THERAPEUT PROGRAM,BETHESDA,MD 20892. NR 56 TC 36 Z9 36 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1995 VL 7 IS 2 BP 83 EP 95 PG 13 WC Oncology SC Oncology GA RC101 UT WOS:A1995RC10100004 PM 7579731 ER PT J AU SINGH, N SUN, Y NAKAMURA, K SMITH, MR COLBURN, NH AF SINGH, N SUN, Y NAKAMURA, K SMITH, MR COLBURN, NH TI C-JUN/AP-1 AS POSSIBLE MEDIATORS OF TUMOR NECROSIS FACTOR-ALPHA-INDUCED APOPTOTIC RESPONSE IN MOUSE JB6 TUMOR-CELLS SO ONCOLOGY RESEARCH LA English DT Article DE MOUSE JB6 CELLS; APOPTOSIS RESPONSE VARIANTS; MOLECULAR MEDIATORS OF TNF-ALPHA RESPONSE ID PROTEIN-KINASE-C; DNA FRAGMENTATION; IMMATURE THYMOCYTES; DEATH; JUN; PHOSPHORYLATION; EXPRESSION; ACTIVATION; FOS; MYC AB Sensitivity to cell killing by tumor necrosis factor (TNF)-alpha was seen in the JB6-derived transformed mouse RT101 cell variants previously described as resistant to 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced killing, while the TPA-sensitive variants were resistant to killing by TNF-alpha. Morphological and biochemical changes characteristic of apoptosis were found to precede TNF-alpha-induced cell death in TNF-alpha-sensitive (TNFs) but not TNF-alpha-resistant (TNFr) cells. In TNFr cells, TNF-alpha increased the cell cycle rate. The onset of cellular damage in TNFs cells, as indicated by propidium iodide uptake, was seen as early as 6 h after TNF-alpha treatment. 4,6-diamidino-2-phenylindole staining revealed chromosomal condensation approximately 46 h after TNF-alpha treatment. The DNA oligonucleosomal ladder of 180 bp and its multiples, a characteristic feature of apoptosis, was seen at 48 h. Little or no significant differences were found in the basal or induced levels of mRNA expression of several potential apoptosis mediator genes or apoptosis inhibitor genes. A dephosphorylated species of anti-c-Jun immunoprecipitated protein appeared in TNFs cells at 3 h posttreatment, accompanied by a parallel increase in AP-1 activity. Higher constitutive levels of the antioxidant enzymes superoxide dismutase and catalase were found in TNFr cells, but TNF-alpha did not significantly affect the activities of these enzymes or differentially induce their expression. The findings suggest that the preferential and transient increase in c-Jun dephosphorylation and AP-1 transcriptional activity may contribute to the preferential apoptotic response in TNFs cells; and that the greater constitutive oxidant defense in TNFr cells may contribute to their resistance. C1 NCI,FREDERICK CANC RES & DEV CTR,VIRAL CARCINOGENESIS LAB,CELL BIOL SECT,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,SAIC FREDERICK,BIOL CARCINOGENESIS & DEV PROGRAM,FREDERICK,MD 21702. NR 43 TC 28 Z9 29 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1995 VL 7 IS 7-8 BP 353 EP 362 PG 10 WC Oncology SC Oncology GA TE772 UT WOS:A1995TE77200004 PM 8747598 ER PT J AU DAS, PC ROBERTS, JD WHITE, SL OLDEN, K AF DAS, PC ROBERTS, JD WHITE, SL OLDEN, K TI ACTIVATION OF RESIDENT TISSUE-SPECIFIC MACROPHAGES BY SWAINSONINE SO ONCOLOGY RESEARCH LA English DT Article DE SWAINSONINE; MACROPHAGE-ACTIVATION; CHEMOTHERAPY; LIPOPOLYSACCHARIDE; INTERFERON-GAMMA; METASTASIS ID PROTEIN-KINASE-C; TUMOR-CELLS; HUMAN-LYMPHOCYTES; NEOPLASTIC-CELLS; MELANOMA-CELLS; MURINE TUMOR; INHIBITION; METASTASIS; INVITRO; GROWTH AB The induction of macrophage tumoricidal activity by swainsonine (8a beta-indolizidine-1 alpha, 2 alpha, 8 beta-tri-ol), an indolizidine alkaloid, has been implicated as possibly an important immune effector mechanism involved in the suppression of tumor growth and metastasis in vital organs such as the lung, liver and spleen (Olden, K. et al. The potential importance of swainsonine in therapy for cancers and immunology. Pharmacol. Ther. 50:285-290; 1991). The present study further explores this possibility by determining whether resident tissue-specific macrophages of several mouse strains can be rendered tumoricidal by systemic administration of swainsonine. We found that systemically administered swainsonine could increase the tumoricidal activity of both alveolar (lung) and splenic macrophages. The activity was enhanced as much as 3- to 4-fold over that obtained with macrophages from organs of control animals and was both dose- and time-dependent. The level and extent of activation by swainsonine was comparable to that achieved with traditional macrophage-activating agents, such as lipopolysaccharide and interferon-gamma. The fact that swainsonine activated highly purified (>95%) cultures of macrophages from the various sources suggests a direct mechanism of activation. Furthermore, the in vivo activation of macrophages in immune-compromised animals (SCID and nude) lends credence to this suggestion. These findings provide a plausible explanation for the observations that systemically administered swainsonine inhibits organ colonization of metastatic cells and growth of SC tumor xenografts, whereas the growth of tumor cells is not inhibited by swainsonine in culture. C1 NIEHS,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709. DUKE UNIV,MED CTR,DEPT MED,DURHAM,NC 27710. NR 52 TC 60 Z9 60 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0965-0407 J9 ONCOL RES JI Oncol. Res. PY 1995 VL 7 IS 9 BP 425 EP 433 PG 9 WC Oncology SC Oncology GA TJ696 UT WOS:A1995TJ69600001 PM 8835286 ER PT J AU INGERSOLL, GL GOODNOUGHHANNEMAN, SK KNEBEL, AR SHEKLETON, ME BURNS, SM CLOCHESY, JM AF INGERSOLL, GL GOODNOUGHHANNEMAN, SK KNEBEL, AR SHEKLETON, ME BURNS, SM CLOCHESY, JM TI MEASUREMENT ISSUES IN MECHANICAL VENTILATION WEANING RESEARCH SO ONLINE JOURNAL OF KNOWLEDGE SYNTHESIS FOR NURSING LA English DT Article DE CONCEPTUAL FRAMEWORK; VENTILATION, MECHANICAL; VENTILATOR WEANING, RESEARCH INSTRUMENTS; RESEARCH METHODS ID MAXIMAL INSPIRATORY PRESSURE; EARLY EXTUBATION; CARDIAC-SURGERY; CRITERIA; EXPERIENCE; SUCCESS; TRIALS; PH AB This article is the fifth in a series written by the Third National Study Group on Weaning from Mechanical Ventilation sponsored by the American Association of Critical-Care Nurses. The authors express appreciation to the membership and Board of Directors of the American Association of Critical-Care Nurses for sponsoring the work of the group and to Dr. Victoria Champion and Dr. Nancy Dayhoff for thoughtful critiques of the manuscript. C1 TEXAS WOMANS UNIV,COLL NURSING,HOUSTON CTR,HOUSTON,TX. NIH,WARREN GRANT MAGNUSON CLIN CTR,BETHESDA,MD 20892. UNIV VIRGINIA,SCH NURSING,CHARLOTTESVILLE,VA 22903. UNIV PITTSBURGH,PITTSBURGH,PA 15260. RP INGERSOLL, GL (reprint author), INDIANA UNIV,SCH MED,1111 MIDDLE DR,INDIANAPOLIS,IN 46202, USA. NR 39 TC 3 Z9 3 U1 0 U2 1 PU SIGMA THETA TAU INT PI INDIANAPOLIS PA 550 W NORTH STREET, INDIANAPOLIS, IN 46202 SN 1072-7639 J9 ONLINE J KNOWL SYN N JI Online J. Knowl. Synth. Nurs. PY 1995 VL 2 IS 24 BP 1 EP 11 PG 11 WC Nursing SC Nursing GA TJ220 UT WOS:A1995TJ22000001 ER PT S AU Young, NS Maciejewski, JP Selleri, C Sato, T Nistico, A Brown, KE Green, SW Wong, S Anderson, S AF Young, NS Maciejewski, JP Selleri, C Sato, T Nistico, A Brown, KE Green, SW Wong, S Anderson, S BE Gluckman, E Coulombel, L TI The pathophysiology of acquired aplastic anemia SO ONTOGENY OF HEMATOPOIESIS: APLASTIC ANEMIA SE COLLOQUE INSERM LA English DT Proceedings Paper CT Joint International Workshop on Foetal and Neonatal Hematopoiesis and Mechanisms of Bone Marrow Failure CY APR 03-06, 1995 CL PARIS, FRANCE SP INSERM, Minist Enseignement Rech, NIH NHLBI, European Haematol Assoc, European Sch Haematol, Assoc Rech Transplantat Medullaires, Medullaires, Assoc Fanconi Anemia RP Young, NS (reprint author), NHLBI,HEMATOL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU EDITIONS INSERM PI PARIS PA 101 RUE DE TOLBIAC, 75645 PARIS, FRANCE SN 0768-3154 BN 2-85598-626-5 J9 COLLOQ INSE PY 1995 VL 235 BP 193 EP 201 PG 9 WC Hematology SC Hematology GA BF80Z UT WOS:A1995BF80Z00027 ER PT S AU Liu, JM AF Liu, JM BE Gluckman, E Coulombel, L TI Molecular approaches to the treatment of Fanconi anemia SO ONTOGENY OF HEMATOPOIESIS: APLASTIC ANEMIA SE COLLOQUE INSERM LA English DT Proceedings Paper CT Joint International Workshop on Foetal and Neonatal Hematopoiesis and Mechanisms of Bone Marrow Failure CY APR 03-06, 1995 CL PARIS, FRANCE SP INSERM, Minist Enseignement Rech, NIH NHLBI, European Haematol Assoc, European Sch Haematol, Assoc Rech Transplantat Medullaires, Medullaires, Assoc Fanconi Anemia RP Liu, JM (reprint author), NIH,BLDG 10 ACRF,ROOM 7C103,BETHESDA,MD 20205, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU EDITIONS INSERM PI PARIS PA 101 RUE DE TOLBIAC, 75645 PARIS, FRANCE SN 0768-3154 BN 2-85598-626-5 J9 COLLOQ INSE PY 1995 VL 235 BP 361 EP 369 PG 9 WC Hematology SC Hematology GA BF80Z UT WOS:A1995BF80Z00048 ER PT B AU SWANGO, PA KLEINMAN, DV KONZELMAN, JL AF SWANGO, PA KLEINMAN, DV KONZELMAN, JL BE Greenspan, JS Greenspan, D TI EPIDEMIOLOGY OF ORAL MANIFESTATIONS OF HIV INFECTION IN A UNITED-STATES MILITARY POPULATION SO ORAL MANIFESTATIONS OF HIV INFECTION LA English DT Proceedings Paper CT 2nd International Workshop on the Oral Manifestations of HIV Infection CY JAN 31-FEB 03, 1993 CL SAN FRANCISCO, CA C1 NIDR,EPIDEMIOL & ORAL DIS PREVENT PROGRAM,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 1 U2 1 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 N KIMBERLY DR, CAROL STREAM, IL 60188-1881 BN 0-86715-286-9 PY 1995 BP 49 EP 59 PG 11 WC Dentistry, Oral Surgery & Medicine; Infectious Diseases SC Dentistry, Oral Surgery & Medicine; Infectious Diseases GA BC73K UT WOS:A1995BC73K00005 ER PT B AU FOX, PC AF FOX, PC BE Greenspan, JS Greenspan, D TI ANTI-HIV-1 ACTIVITY IN HUMAN SALIVA SO ORAL MANIFESTATIONS OF HIV INFECTION LA English DT Proceedings Paper CT 2nd International Workshop on the Oral Manifestations of HIV Infection CY JAN 31-FEB 03, 1993 CL SAN FRANCISCO, CA C1 NIDR,CLIN INVEST & PATIENT CARE BRANCH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 N KIMBERLY DR, CAROL STREAM, IL 60188-1881 BN 0-86715-286-9 PY 1995 BP 130 EP 137 PG 8 WC Dentistry, Oral Surgery & Medicine; Infectious Diseases SC Dentistry, Oral Surgery & Medicine; Infectious Diseases GA BC73K UT WOS:A1995BC73K00016 ER PT B AU PLUDA, JM SAVILLE, MW FOLI, A BRODER, S YARCHOAN, R AF PLUDA, JM SAVILLE, MW FOLI, A BRODER, S YARCHOAN, R BE Greenspan, JS Greenspan, D TI THE PATHOGENESIS AND TREATMENT OF HUMAN IMMUNODEFICIENCY-ASSOCIATED KAPOSIS SARCOMA SO ORAL MANIFESTATIONS OF HIV INFECTION LA English DT Proceedings Paper CT 2nd International Workshop on the Oral Manifestations of HIV Infection CY JAN 31-FEB 03, 1993 CL SAN FRANCISCO, CA C1 NCI,INT DRUG BRANCH,CANC THERAPY EVALUAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 N KIMBERLY DR, CAROL STREAM, IL 60188-1881 BN 0-86715-286-9 PY 1995 BP 200 EP 204 PG 5 WC Dentistry, Oral Surgery & Medicine; Infectious Diseases SC Dentistry, Oral Surgery & Medicine; Infectious Diseases GA BC73K UT WOS:A1995BC73K00025 ER PT B AU KINNARD, MA AF KINNARD, MA BE Greenspan, JS Greenspan, D TI SUMMARY AND RECOMMENDATIONS FROM THE 2ND INTERNATIONAL WORKSHOP ON THE ORAL MANIFESTATIONS OF HIV INFECTION SO ORAL MANIFESTATIONS OF HIV INFECTION LA English DT Proceedings Paper CT 2nd International Workshop on the Oral Manifestations of HIV Infection CY JAN 31-FEB 03, 1993 CL SAN FRANCISCO, CA C1 NIDR,OFF DIRECTOR,EXTRAMURAL ASSOCIATES PROGRAM,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 N KIMBERLY DR, CAROL STREAM, IL 60188-1881 BN 0-86715-286-9 PY 1995 BP 366 EP 370 PG 5 WC Dentistry, Oral Surgery & Medicine; Infectious Diseases SC Dentistry, Oral Surgery & Medicine; Infectious Diseases GA BC73K UT WOS:A1995BC73K00051 ER PT J AU DUBNER, R AF DUBNER, R TI HYPERALGESIA IN RESPONSE TO INJURY TO CUTANEOUS AND DEEP TISSUES SO OROFACIAL PAIN AND TEMPOROMANDIBULAR DISORDERS SE ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID DORSAL HORN NEURONS; INSITU HYBRIDIZATION HISTOCHEMISTRY; EXPERIMENTAL PERIPHERAL NEUROPATHY; MULTIPLE OPIOID SYSTEMS; SPINAL-CORD; GENE-EXPRESSION; CONSTRICTION INJURY; RECEPTIVE-FIELDS; MUSCLE AFFERENTS; VISCERAL INPUTS RP DUBNER, R (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 30,BETHESDA,MD 20892, USA. NR 47 TC 6 Z9 6 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0146-0722 J9 ADV PAIN RES THER PY 1995 VL 21 BP 61 EP 71 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA BC04F UT WOS:A1995BC04F00005 ER PT J AU GRACELY, RH REID, KI AF GRACELY, RH REID, KI TI OROFACIAL PAIN MEASUREMENT SO OROFACIAL PAIN AND TEMPOROMANDIBULAR DISORDERS SE ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review ID EVENT-RELATED POTENTIALS; TOOTH-PULP STIMULATION; TRIGGER POINT SENSITIVITY; BURNING MOUTH SYNDROME; NITROUS-OXIDE; ELECTRICAL-STIMULATION; TEMPOROMANDIBULAR-JOINT; PRESSURE ALGOMETER; EVOKED-POTENTIALS; MUSCLE TENDERNESS C1 UNIV KENTUCKY,ALBERT B CHANDLER MED CTR,COLL DENT,CTR OROFACIAL PAIN,LEXINGTON,KY 40536. RP GRACELY, RH (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892, USA. NR 97 TC 7 Z9 7 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0146-0722 J9 ADV PAIN RES THER PY 1995 VL 21 BP 117 EP 143 PG 27 WC Medicine, General & Internal SC General & Internal Medicine GA BC04F UT WOS:A1995BC04F00009 ER PT S AU MUKHERJEE, BB NEMIR, M BENINATI, S CORDELLAMIELE, E SINGH, K CHACKALAPARAMPIL, I SHANMUGAM, V DEVOUGE, MW MUKHERJEE, AB AF MUKHERJEE, BB NEMIR, M BENINATI, S CORDELLAMIELE, E SINGH, K CHACKALAPARAMPIL, I SHANMUGAM, V DEVOUGE, MW MUKHERJEE, AB BE Denhardt, DT Butler, WT Chambers, AF Senger, DR TI INTERACTION OF OSTEOPONTIN WITH FIBRONECTIN AND OTHER EXTRACELLULAR-MATRIX MOLECULES SO OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Osteopontin - Role in Cell Signalling and Adhesion CY OCT 21-23, 1994 CL NEW BRUNSWICK, NJ SP New York Acad Sci ID TRANSFORMED MAMMALIAN-CELLS; RAT-KIDNEY CELLS; PHYSIOLOGICAL-PROPERTIES; NONPHOSPHORYLATED FORMS; BLOOD-COAGULATION; CROSS-LINKING; FACTOR-XIII; PHOSPHOPROTEIN; BONE; CLONING C1 MCGILL UNIV,DEPT HUMAN GENET,MONTREAL,PQ H3A 1B1,CANADA. NICHHD,HUMAN GENET BRANCH,DEV GENET SECT,BETHESDA,MD 20892. RP MUKHERJEE, BB (reprint author), MCGILL UNIV,DEPT BIOL,1205 DR PENFIELD AVE,MONTREAL,PQ H3A 1B1,CANADA. OI Beninati, Simone/0000-0002-2704-0745 NR 27 TC 48 Z9 49 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-939-8 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 760 BP 201 EP 212 DI 10.1111/j.1749-6632.1995.tb44631.x PG 12 WC Biochemistry & Molecular Biology; Cell Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Science & Technology - Other Topics GA BD12E UT WOS:A1995BD12E00018 PM 7785895 ER PT S AU OMIGBODUN, A DAITER, E WALINSKY, D FISHER, L YOUNG, M HOYER, J COUTIFARIS, C AF OMIGBODUN, A DAITER, E WALINSKY, D FISHER, L YOUNG, M HOYER, J COUTIFARIS, C BE Denhardt, DT Butler, WT Chambers, AF Senger, DR TI REGULATED EXPRESSION OF OSTEOPONTIN IN HUMAN TROPHOBLASTS SO OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Osteopontin - Role in Cell Signalling and Adhesion CY OCT 21-23, 1994 CL NEW BRUNSWICK, NJ SP New York Acad Sci ID DIFFERENTIATION C1 HOSP UNIV PENN,DEPT OBSTET & GYNECOL,DIV HUMAN REPROD,PHILADELPHIA,PA 19104. NIDR,BONE RES BRANCH,BETHESDA,MD. NR 9 TC 9 Z9 9 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-939-8 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 760 BP 346 EP 349 DI 10.1111/j.1749-6632.1995.tb44653.x PG 4 WC Biochemistry & Molecular Biology; Cell Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Science & Technology - Other Topics GA BD12E UT WOS:A1995BD12E00040 PM 7785913 ER PT J AU LOOKER, AC WAHNER, HW DUNN, WL CALVO, MS HARRIS, TB HEYSE, SP JOHNSTON, CC LINDSAY, RL AF LOOKER, AC WAHNER, HW DUNN, WL CALVO, MS HARRIS, TB HEYSE, SP JOHNSTON, CC LINDSAY, RL TI PROXIMAL FEMUR BONE-MINERAL LEVELS OF US ADULTS SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE BONE MINERAL DENSITY; PROXIMAL FEMUR ID BODY HABITUS; DENSITY; WOMEN; HIP; SPINE; RACE AB This paper describes bone mineral levels in the proximal femur of US adults based on a nationally representative sample of 7116 men and women aged 20 years and older. The data were collected in phase 1 of the third National Health and Nutrition Examination Survey (NHANES III, 1988-1991) using dual-energy X-ray absorptiometry, and included bone mineral density (EMD), bone mineral content (BMC) and area of bone scanned in five selected regions of interest (ROI) in the proximal femur: femur neck, trochanter, intertrochanter, Ward's triangle and total. These variables are provided separately by age and sex for non-Hispanic whites (NHW), non-Hispanic blacks (NHB) and Mexican Americans (MA). BMD and BMC in the five ROI tended to decline with age, whereas area did not. BMD and BMC were highest in NHB, intermediate in MA and lowest in NHW, but areas were highest in NHW, intermediate in NHB and lowest in MA. Men had greater BMD, BMC and area than women in all three race/ethnic groups. Differences by age, sex or race/ethnicity tended to be the largest in Ward's triangle, followed by the femur neck; patterns in the trochanter, intertrochanter and total ROI were reasonably similar to each other. This report provides extensive data on femur bone mineral levels of adults from one of the largest samples available to date and should be valuable as reference data for other studies which examine this skeletal site in adults. C1 MAYO CLIN & MAYO FDN,ROCHESTER,MN 55905. US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204. NIA,BETHESDA,MD 20892. NIAMSD,BETHESDA,MD 20892. INDIANA UNIV,MED CTR,INDIANAPOLIS,IN. HELEN HAYES HOSP,CTR REG BONE,W HAVERSTRAW,NY. RP LOOKER, AC (reprint author), CTR DIS CONTROL & PREVENT,NATL CTR HLTH STAT,ROOM 900,6525 BELCREST RD,HYATTSVILLE,MD 20782, USA. NR 24 TC 236 Z9 237 U1 0 U2 2 PU SPRINGER-VERLAG LONDON LTD PI GODALMING PA SWEETAPPLE HOUSE CATTESHALL ROAD, GODALMING, SURREY, ENGLAND GU7 3DJ SN 0937-941X J9 OSTEOPOROSIS INT JI Osteoporosis Int. PY 1995 VL 5 IS 5 BP 389 EP 409 DI 10.1007/BF01622262 PG 21 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RX678 UT WOS:A1995RX67800009 PM 8800790 ER PT B AU CUTLER, RG AF CUTLER, RG BE Cutler, RG Packer, L Bertram, J Mori, A TI Longevity determinant genes, cellular dysdifferentiation and oxidative stress SO OXIDATIVE STRESS AND AGING SE MOLECULAR AND CELL BIOLOGY UPDATES LA English DT Proceedings Paper CT 1st International Conference on Oxidative Stress and Aging CY 1994 CL HI SP Soc Free Radical Res Asia, Oxygen Soc RP Cutler, RG (reprint author), NIA,GERONTOL RES CTR,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA PO BOX 133, CH-4010 BASEL, SWITZERLAND BN 3-7643-5039-3 J9 MCBU PY 1995 BP 15 EP 19 PG 5 WC Cell Biology SC Cell Biology GA BD46L UT WOS:A1995BD46L00002 ER PT B AU BOHR, VA TAFFE, BG LARMINAT, F AF BOHR, VA TAFFE, BG LARMINAT, F BE Cutler, RG Packer, L Bertram, J Mori, A TI DNA repair, oxidative stress and aging SO OXIDATIVE STRESS AND AGING SE MOLECULAR AND CELL BIOLOGY UPDATES LA English DT Proceedings Paper CT 1st International Conference on Oxidative Stress and Aging CY 1994 CL HI SP Soc Free Radical Res Asia, Oxygen Soc RP NIA,GENET MOLEC LAB,4940 EASTERN AVE,BALTIMORE,MD 21224, USA. NR 0 TC 6 Z9 6 U1 0 U2 0 PU BIRKHAUSER VERLAG PI BASEL PA PO BOX 133, CH-4010 BASEL, SWITZERLAND BN 3-7643-5039-3 J9 MCBU PY 1995 BP 101 EP 110 PG 10 WC Cell Biology SC Cell Biology GA BD46L UT WOS:A1995BD46L00012 ER PT S AU Gainer, H Jeong, SW Witt, DM Chin, H AF Gainer, H Jeong, SW Witt, DM Chin, H BE Ivell, R Russell, JA TI Strategies for cell biological studies in oxytocinergic neurons SO OXYTOCIN: CELLULAR AND MOLECULAR APPROACHES IN MEDICINE AND RESEARCH SE Advances in Experimental Medicine and Biology LA English DT Proceedings Paper CT Hanseatic Endocrine Conference on Oxytocin - Cellular and Molecular Approaches in Medicine and Research CY APR 30-MAY 04, 1995 CL STADE, GERMANY SP German Res Council, Ferring Co, Swedish Div, Ferring Co, German Div, GEFEF Fdn, Univ Hamburg, Inst Hormone & Fertil Res ID ALPHA-AMIDATING MONOOXYGENASE; INSITU HYBRIDIZATION; CARBOXYPEPTIDASE-H; TRANSGENIC MICE; GENE-EXPRESSION; NEUROPEPTIDE BIOSYNTHESIS; ORGANOTYPIC CULTURES; BRATTLEBORO RATS; NERVOUS-TISSUE; MESSENGER-RNA AB Hypothalamic magnocellular neurons expressing the neurohypophysial peptides, oxytocin (OT) and vasopressin (AVP), have historically served as valuable models for cell biological studies of peptidergic neurons. This is because of the relative ease with which the perikarya, axons, and nerve terminals of these cells can be isolated for anatomical, biochemical, and physiological analysis. A popular strategy is to harness the regulatory elements in the OT and AVP genes in order to target specific molecules to these cells in vivo, so as to elucidate various cell biological issues, e.g., pacemaker, prohormone processing, axonal transport, and secretion mechanisms. The peptide genes are good sources for regulatory controls since they are relatively abundantly and specifically expressed in these neurons in the CNS. The major problem has been to identify the critical regulatory elements in these genes which are responsible for the cell-specific expression. Recent transgenic studies have implicated various 3'-untranslated nucleotide sequence regions in the OT and AVP genes which appear to contain these regulatory elements. A structural analysis of these regions in the mouse genes will be discussed. A second problem has been the availability of appropriate model systems to test constructs for ''cell-specific'' expression. Transgenic mice have been effective models in this regard but are costly and labor-intensive. An alternative approach using biolistic techniques to transfect slice-explants containing the primary OT and AVP neurons in culture will be described. RP Gainer, H (reprint author), NINCDS, NEUROCHEM LAB, NIH, BETHESDA, MD 20892 USA. NR 58 TC 6 Z9 6 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45290-1 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1995 VL 395 BP 1 EP 8 PG 8 WC Endocrinology & Metabolism; Medicine, Research & Experimental; Reproductive Biology SC Endocrinology & Metabolism; Research & Experimental Medicine; Reproductive Biology GA BF30K UT WOS:A1995BF30K00001 PM 8713947 ER PT J AU LITTLE, RE MACGILLIVRAY, I AF LITTLE, RE MACGILLIVRAY, I TI ABSTINENCE FROM ALCOHOL BEFORE PREGNANCY AND REPRODUCTIVE OUTCOME SO PAEDIATRIC AND PERINATAL EPIDEMIOLOGY LA English DT Discussion ID BIRTH-WEIGHT; CONSUMPTION; WOMEN C1 UNIV BRISTOL,INST CHILD HLTH,BRISTOL BS8 1TH,AVON,ENGLAND. RP LITTLE, RE (reprint author), NIEHS,EPIDEMIOL BRANCH,RES TRIANGLE PK,NC 27709, USA. NR 12 TC 4 Z9 4 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0269-5022 J9 PAEDIATR PERINAT EP JI Paediatr. Perinat. Epidemiol. PD JAN PY 1995 VL 9 IS 1 BP 105 EP 108 PG 4 WC Public, Environmental & Occupational Health; Obstetrics & Gynecology; Pediatrics SC Public, Environmental & Occupational Health; Obstetrics & Gynecology; Pediatrics GA QC662 UT WOS:A1995QC66200011 PM 7724409 ER PT J AU GRACELY, RH AF GRACELY, RH TI HYPNOSIS AND HIERARCHICAL PAIN CONTROL-SYSTEMS SO PAIN LA English DT Editorial Material ID ANALGESIA RP GRACELY, RH (reprint author), NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BLDG 10,ROOM 1N-103,BETHESDA,MD 20892, USA. NR 6 TC 7 Z9 7 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3959 J9 PAIN JI Pain PD JAN PY 1995 VL 60 IS 1 BP 1 EP 2 DI 10.1016/0304-3959(94)00137-4 PG 2 WC Anesthesiology; Clinical Neurology; Neurosciences SC Anesthesiology; Neurosciences & Neurology GA QC525 UT WOS:A1995QC52500001 PM 7715935 ER PT S AU KENSHALO, DR DOUGLASS, DK AF KENSHALO, DR DOUGLASS, DK BE Bromm, B Desmedt, JE TI THE ROLE OF THE CEREBRAL-CORTEX IN THE EXPERIENCE OF PAIN SO PAIN AND THE BRAIN: FROM NOCICEPTION TO COGNITION SE ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Review CT From Nociception to Pain Symposium CY AUG 27-29, 1993 CL BEAUNE, FRANCE SP Bayer, Boehringer Ingelheim, Byk Gulden, Cascan, Glaxo, Godecke, Hoechst, Knoll AG, Krewel, Lichtwer, Merck Darmstadt, Merck Sharp & Dohme, Mundipharma, Nordmark, Philips, Sandoz, Schwarz Pharma ID TOOTH-PULP AFFERENTS; SOMATOSENSORY CORTEX; NUCLEUS SUBMEDIUS; M FASCICULARIS; NOCICEPTIVE NEURONS; INTRACTABLE PAIN; DRIVEN NEURONS; CORTICAL-CELLS; STIMULATION; CAT AB In the early 1900s, Head and Holmes observed that damage to the parietal lobes produced at most a temporary loss in pain sensation. On this basis they suggested that the cerebral cortex plays little role in the experience of pain. Evidence from more recent studies has reversed this belief. The technique of positron-emission tomography (PET) has been used in humans to show that several cortical areas exhibit a response to acute painful stimuli that is not seen with neutral, warm, or vibrotactile stimuli, suggesting that these areas are indeed involved in the perception of pain. These cortical areas include the primary somatosensory cortex (SI), secondary somatosensory cortex (SII), insula, and cingulate cortex. The primary somatosensory cortex appears to be involved in the sensory-discriminative aspect of pain. Clinical lesions in humans and experimental lesion studies in primates have shown that SI is involved in the ability to detect and discriminate changes in the intensity of painful stimuli. Electrophysiological studies in monkeys and rats have demonstrated neurons in SI that respond to and can encode the intensity of noxious stimuli. Other cortical areas that have been implicated in pain perception appear to be involved in aspects of pain other than the sensory-discriminative component. Nociceptive neurons in SII and Area 7b tend to have large, sometimes bilateral receptive fields (RFs) and often respond to auditory and visual stimuli. These data suggest that these areas may be important in spatially directed attention to noxious stimuli. The anterior cingulate cortex is considered part of the medial pain system, and there is evidence from both clinical observations and animal studies that it may be concerned with the affective-motivational aspect of pain perception, though this has yet to be directly tested. Nociceptive neurons in the ventrolateral orbital cortex have been shown to respond to noxious stimulation of skin, viscera, and joints; to have large, often bilateral RFs and to lack somatotopic organization. It is suggested that this area may also play a role in the affective-motivational component of pain perception. RP NIDR, NEUROBIOL & ANESTHESIOL BRANCH, BLDG 30, BETHESDA, MD 20892 USA. NR 76 TC 22 Z9 23 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 USA SN 0146-0722 BN 0-7817-0322-0 J9 ADV PAIN RES THER PY 1995 VL 22 BP 21 EP 34 PG 14 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA BE25E UT WOS:A1995BE25E00002 ER PT S AU MAX, MB AF MAX, MB BE Bromm, B Desmedt, JE TI ANTIDEPRESSANT DRUGS AS TREATMENTS FOR CHRONIC PAIN - EFFICACY AND MECHANISMS SO PAIN AND THE BRAIN: FROM NOCICEPTION TO COGNITION SE ADVANCES IN PAIN RESEARCH AND THERAPY LA English DT Article; Proceedings Paper CT From Nociception to Pain Symposium CY AUG 27-29, 1993 CL BEAUNE, FRANCE SP Bayer, Boehringer Ingelheim, Byk Gulden, Cascan, Glaxo, Godecke, Hoechst, Knoll AG, Krewel, Lichtwer, Merck Darmstadt, Merck Sharp & Dohme, Mundipharma, Nordmark, Philips, Sandoz, Schwarz Pharma ID DIABETIC NEUROPATHY SYMPTOMS; RELIEVES POSTHERPETIC NEURALGIA; PLACEBO-CONTROLLED TRIAL; SEROTONIN REUPTAKE; DOUBLE-BLIND; IMIPRAMINE TREATMENT; MORPHINE ANALGESIA; AMITRIPTYLINE; DESIPRAMINE; CLOMIPRAMINE AB Apart from the aspirin-like drugs and opioids, tricyclic antidepressants are probably the class of drugs most commonly used to treat chronic pain. Although about 60 controlled clinical trials have been done with these drugs, evidence of their efficacy is conclusive only for diabetic neuropathy and postherpetic neuralgia, with a likely effect in fibromyalgia, tension and migraine headache, and atypical facial pain. This chapter focuses on the 16 trials of tricyclic antidepressants in neuropathic pain, from which the following conclusions may be drawn: (a) Although improvement in mood may contribute to reports of decreased pain in some patients, there is clear evidence for pain relief in patients without preexisting mood disorders. (b) Several different types of painful symptoms are relieved, including brief and steady spontaneous pains of various qualities, and light touch-evoked allodynia. (c) Drugs that block both serotonin 5-hydroxytryptamine (5HT) and norepinephrine (NE) reuptake, such as amitriptyline and imipramine, appear superior to drugs with selective actions on one of these neurotransmitters. However, the relatively selective NE reuptake blocker desipramine has consistently shown efficacy in four clinical trials. Some selective 5HT reuptake blockers (paroxetine, citalopram), but not others (fluoxetine, zimelidine), have surpassed placebo in single clinical trials. Whether other actions of the first-generation tricyclic antidepressants, such as blockade of muscarinic, adrenergic, or histaminic receptors, play a role in analgesia remains unclear, but may be elucidated by further comparisons with new and more selective agents. RP MAX, MB (reprint author), NIH,NEUROBIOL & ANESTHESIOL BRANCH,CLIN TRIALS UNIT,BETHESDA,MD 20892, USA. NR 53 TC 12 Z9 12 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0146-0722 BN 0-7817-0322-0 J9 ADV PAIN RES THER PY 1995 VL 22 BP 501 EP 515 PG 15 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA BE25E UT WOS:A1995BE25E00033 ER PT B AU DIPAOLO, JA CHEN, MC POPESCU, NC WOODWORTH, CD ZIMONJIC, DB AF DIPAOLO, JA CHEN, MC POPESCU, NC WOODWORTH, CD ZIMONJIC, DB BE Monsonego, J TI Interrelationship of human papillomavirus, human herpesvirus and human immunodeficiency to cervical neoplasia SO PAPILLOMAVIRUS IN HUMAN PATHOLOGY SE CHALLENGES OF MODERN MEDICINE LA English DT Proceedings Paper CT 2nd International Congress on Papillomavirus in Human Pathology CY APR 06-08, 1994 CL PARIS, FRANCE SP French Minist Hlth, UNESCO, WHO, European Union RP NCI,BIOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ARES-SERONO SYMPOSIA PUBLICATIONS PI ROME PA VIA RAVENNA 8, 00161 ROME, ITALY BN 88-85974-20-1 J9 CHALL MOD MED PY 1995 VL 9 BP 55 EP 64 PG 10 WC Oncology; Obstetrics & Gynecology; Virology SC Oncology; Obstetrics & Gynecology; Virology GA BC71Z UT WOS:A1995BC71Z00005 ER PT B AU DIPAOLO, JA WOODWORTH, CD EVANS, CH AF DIPAOLO, JA WOODWORTH, CD EVANS, CH BE Monsonego, J TI Cytokines inhibit human papillomavirus transcription and enhance cell sensitivity to lymphocyte killing SO PAPILLOMAVIRUS IN HUMAN PATHOLOGY SE CHALLENGES OF MODERN MEDICINE LA English DT Proceedings Paper CT 2nd International Congress on Papillomavirus in Human Pathology CY APR 06-08, 1994 CL PARIS, FRANCE SP French Minist Hlth, UNESCO, WHO, European Union RP NCI,BIOL LAB,BLDG 37,ROOM 2A19,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ARES-SERONO SYMPOSIA PUBLICATIONS PI ROME PA VIA RAVENNA 8, 00161 ROME, ITALY BN 88-85974-20-1 J9 CHALL MOD MED PY 1995 VL 9 BP 449 EP 458 PG 10 WC Oncology; Obstetrics & Gynecology; Virology SC Oncology; Obstetrics & Gynecology; Virology GA BC71Z UT WOS:A1995BC71Z00053 ER PT J AU RILEY, EM WILLIAMSON, KC GREENWOOD, BM KASLOW, DC AF RILEY, EM WILLIAMSON, KC GREENWOOD, BM KASLOW, DC TI HUMAN IMMUNE RECOGNITION OF RECOMBINANT PROTEINS REPRESENTING DISCRETE DOMAINS OF THE PLASMODIUM-FALCIPARUM GAMETE SURFACE PROTEIN, PFS230 SO PARASITE IMMUNOLOGY LA English DT Article DE MALARIA; TRANSMISSION BLOCKING IMMUNITY; PFS230; PLASMODIUM-FALCIPARUM; GAMETES ID TRANSMISSION-BLOCKING ANTIBODIES; SEXUAL STAGES; MALARIA TRANSMISSION; TARGET ANTIGEN; T-CELL; GALLINACEUM; RESPONSES; EXPRESSION; INFECTION; PARASITES AB The 230 kD gametocyte/gamete-specific surface protein of Plasmodium falciparum, Pfs230, is a target of antibodies which inhibit the development of the parasite inside the mosquito vector. A transmission blocking vaccine based on Pfs230 may be a powerful tool for malaria control. As a first step, Pfs230 has been expressed in E. coli as a series of recombinant proteins,fused to maltose binding protein. We have used the fusion proteins to assess cellular and humoral immune responses to Pfs230 in malaria-immune adult Gambian blood donors; responses to the fusion proteins have been compared with responses to native Pfs230. The tetrapeptide repeat region of the molecule appears to be immunodominant for both antibody-producing cells and peripheral blood T cells. We postulate that this may represent a mechanism for immune evasion since the N-terminal repeat region of the molecule is cleaved from the mature protein and shed into the plasma. Responses to fusion proteins representing the seven-cysteine motifs were correlated within individual donors, suggesting that cross-reactive epitopes occur within the motifs. Antibody responses to recombinant proteins were poorly correlated with responses to native Pfs230 suggesting that dominant epitopes of the native protein are not adequately represented in the recombinant proteins. Although prokaryotic expression products may be suitable for induction of cellular immune responses to Pfs230, alternative expression systems may be needed for creation of appropriate B cell epitopes. C1 NIAID,MALARIA RES LAB,MOLEC VACCINE SECT,BETHESDA,MD 20892. MRC LABS,FAJARA,GAMBIA. RP RILEY, EM (reprint author), UNIV EDINBURGH,INST CELL ANIM & POPULAT BIOL,DIV BIOL SCI,W MAINS RD,EDINBURGH EH9 3JT,MIDLOTHIAN,SCOTLAND. RI Riley, Eleanor/C-8960-2013 OI Riley, Eleanor/0000-0003-3447-3570 FU Wellcome Trust NR 26 TC 20 Z9 22 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0141-9838 J9 PARASITE IMMUNOL JI Parasite Immunol. PD JAN PY 1995 VL 17 IS 1 BP 11 EP 19 DI 10.1111/j.1365-3024.1995.tb00961.x PG 9 WC Immunology; Parasitology SC Immunology; Parasitology GA QG755 UT WOS:A1995QG75500002 PM 7731731 ER PT J AU LINDO, JF ROBINSON, RD TERRY, SI VOGEL, P GAM, AA NEVA, FA BUNDY, DAP AF LINDO, JF ROBINSON, RD TERRY, SI VOGEL, P GAM, AA NEVA, FA BUNDY, DAP TI AGE-PREVALENCE AND HOUSEHOLD CLUSTERING OF STRONGYLOIDES-STERCORALIS INFECTION IN JAMAICA SO PARASITOLOGY LA English DT Article DE STRONGYLOIDES STERCORALIS; PREVALENCE; HOUSEHOLD CLUSTERING; EPIDEMIOLOGY ID POPULATION; CARRIERS; FEATURES AB The epidemiology of Strongyloides stercoralis was studied in families of clinical (reference) cases and their neighbours at endemic foci in Jamaica. Thirteen foci were studied based on the place of residence of a reference case. For each household of a reference case, the 4 most proximal neighbourhood households (spatial controls) were included in the study. Out of 312 persons contacted 244 were followed up using questionnaires, stool examination and serology. Prevalence of infection based on stool examination was 3.5 % and on ELISA 24.2 %. Prevalence increased with age but was not related to gender. Reference cases were significantly older than the general study population. The prevalence of infection based on both serology and stool examination was significantly higher in reference than in neighbouring households (the reference cases, themselves, were not included in the analysis). Furthermore, prevalence of infection was highest among persons who shared a bedroom with a reference case and decreased significantly with increasing spatial separation. This is indicative of close contact transmission which has not been previously shown for a geohelminth, but which is common among microparasites. C1 UNIV W INDIES,DEPT ZOOL,KINGSTON 7,JAMAICA. UNIV W INDIES,DEPT MED,KINGSTON 7,JAMAICA. NIAID,BETHESDA,MD 20892. UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOL,WELLCOME RES CTR PARASIT INFECT,LONDON SW7 2BB,ENGLAND. FU Wellcome Trust NR 24 TC 27 Z9 29 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0031-1820 J9 PARASITOLOGY JI Parasitology PD JAN PY 1995 VL 110 BP 97 EP 102 PN 1 PG 6 WC Parasitology SC Parasitology GA QC656 UT WOS:A1995QC65600012 PM 7845718 ER PT J AU LEVAV, M MIRSKY, AF SCHANTZ, PM CASTRO, S CRUZ, ME AF LEVAV, M MIRSKY, AF SCHANTZ, PM CASTRO, S CRUZ, ME TI PARASITIC INFECTION IN MALNOURISHED SCHOOL-CHILDREN - EFFECTS ON BEHAVIOR AND EEG SO PARASITOLOGY LA English DT Article DE NEUROPSYCHOLOGICAL TESTS; PARASITE INFECTION; MALNUTRITION; EEG ID TRICHURIS-TRICHIURA; GROWTH AB This paper describes a study of 194 children (aged 9-13) from a mountain village in Ecuador who were infected with one or more species of intestinal helminth or protozoan parasite. In addition to parasite load, the assessment consisted of a battery of psychological and neuropsychological tests, an EEG examination, measures of iodine level, presence of goitre and level of nutrition. We found that, in general, parasite infection, as measured at the baseline level, was not associated with cognitive impairment. The intensity of infection with A. lumbricoides, however, was correlated with the level of verbal ability and with inhibition-control aspects of cognitive behaviour. Multivariate analysis with level of nutrition, EEG status and parasite burden showed a consistent main effect of the degree of nutrition on neuropsychological performance, particularly the language, problem solving and inhibition-control dimensions. C1 CTR DIS CONTROL, PARASIT DIS BRANCH, ATLANTA, GA 30333 USA. ACAD ECUATORIANA NEUROCIENCIAS, QUITO, ECUADOR. RP LEVAV, M (reprint author), NIMH, PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C110, 10 CTR DR, MSC 1366, BETHESDA, MD 20892 USA. NR 37 TC 26 Z9 27 U1 1 U2 2 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0031-1820 J9 PARASITOLOGY JI Parasitology PD JAN PY 1995 VL 110 BP 103 EP 111 PN 1 PG 9 WC Parasitology SC Parasitology GA QC656 UT WOS:A1995QC65600013 PM 7845707 ER PT J AU KARRON, RA STEINHOFF, MC SUBBARAO, EK WILSON, MH MACLEOD, K CLEMENTS, ML FRIES, LF MURPHY, BR CLEMENTS, ML AF KARRON, RA STEINHOFF, MC SUBBARAO, EK WILSON, MH MACLEOD, K CLEMENTS, ML FRIES, LF MURPHY, BR CLEMENTS, ML TI SAFETY AND IMMUNOGENICITY OF A COLD-ADAPTED INFLUENZA-A (H1N1) REASSORTANT VIRUS-VACCINE ADMINISTERED TO INFANTS LESS-THAN-6 MONTHS OF AGE SO PEDIATRIC INFECTIOUS DISEASE JOURNAL LA English DT Article DE INFLUENZA A VIRUS VACCINE; H1N1 REASSORTANT VIRUS VACCINE; ROUTINE IMMUNIZATION ID YOUNG-CHILDREN; A-VIRUS; AVIAN-HUMAN; INFECTION; IMMUNIZATION; H3N2 AB A safe and effective influenza vaccine is needed to prevent serious influenza illness in infants younger than 6 months of age. The purpose of this study was to determine whether two doses of the cold-adapted (ca) influenza A reassortant vaccine would be safe and immunogenic in this age group. In the first part of this study, infants received two doses of 10(5) or 10(6) 50% tissue culture-infectious dose (TCID50) of the ca influenza vaccine separately from routine immunizations. In the second part of this study two 10(6) TCID50 doses of the ca influenza vaccine were given with routine immunizations at 2 and 4 or 2 and 6 months of age. The ca influenza vaccine was well-tolerated by participants in both parts of this study. Two doses of the ca influenza vaccine were immunogenic in infants who received them separately from routine immunizations; 83% of vaccinees developed protective titers of serum hemagglutination-inhibition (HAI) antibody. In contrast, when the ca vaccine was administered with routine immunizations, protective HAI antibody titers were induced in only 20% of those immunized at 2 and 4 months of age and 50% of those immunized at 2 and 6 months of age. There were no statistically significant associations between HAI antibody response to ca influenza vaccination and dose schedule, presence of passively acquired maternal HAI antibody, ethnic group or breast-feeding status, Young age at the time of first immunization, however, appeared to correlate with decreased response to the hemagglutinin antigen of the influenza A virus. Further studies are needed to determine whether a larger dose of vaccine (10(7) TCID50) could be used safely to improve the serum antibody response to influenza in very young infants. C1 JOHNS HOPKINS UNIV, SCH MED, DEPT PEDIAT, BALTIMORE, MD 21205 USA. JOHNS HOPKINS UNIV, SCH MED, DEPT MED, BALTIMORE, MD 21205 USA. NIAID, INFECT DIS LAB, BETHESDA, MD 20892 USA. PEDIAT CARE CTR, BETHESDA, MD 20814 USA. RP KARRON, RA (reprint author), JOHNS HOPKINS UNIV, SCH HYG & PUBL HLTH, DEPT INT HLTH, CTR IMMUNIZAT RES, BALTIMORE, MD 21205 USA. FU NIAID NIH HHS [N01-AI-15095] NR 24 TC 48 Z9 49 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0891-3668 EI 1532-0987 J9 PEDIATR INFECT DIS J JI Pediatr. Infect. Dis. J. PD JAN PY 1995 VL 14 IS 1 BP 10 EP 16 DI 10.1097/00006454-199501000-00002 PG 7 WC Immunology; Infectious Diseases; Pediatrics SC Immunology; Infectious Diseases; Pediatrics GA QB322 UT WOS:A1995QB32200002 PM 7715982 ER PT J AU FRANKS, RR RAY, PE BABBOTT, CC BRYANT, JL NOTKINS, AL SANTORO, TJ KLOTMAN, PE AF FRANKS, RR RAY, PE BABBOTT, CC BRYANT, JL NOTKINS, AL SANTORO, TJ KLOTMAN, PE TI MATERNAL-FETAL INTERACTIONS AFFECT GROWTH OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TRANSGENIC MICE SO PEDIATRIC RESEARCH LA English DT Article ID NECROSIS-FACTOR-ALPHA; TO-CHILD TRANSMISSION; LONG TERMINAL REPEAT; HIV-INFECTION; GLUCOCORTICOID RECEPTOR; PERINATAL TRANSMISSION; INFANTS; HORMONE; MALNUTRITION; DYSFUNCTION AB Infants vertically infected with human immunodeficiency virus type 1 (HIV-1) often manifest profoundly deficient growth with failure to thrive. The pathologic mechanisms that produce growth failure associated with pediatric HIV infection are not clear. Transgenic mice homozygous for a gag/pol deletion mu tant of the infectious provirus pNL4-3 have been found to manifest a similar growth failure pattern. To explore the influence of HIV-1 on fetal growth and maternal-fetal interactions, we examined intrauterine growth of transgenic and nontransgenic mice and evaluated the consequence of embryo transfer into normal and heterozygous transgenic mothers. Mice homozygous for the HIV transgene had normal intrauterine and birth weights but uniformly displayed severe growth retardation postnatally. Transgene expression was prominent in transgenic fetuses and their placentas and in uteri of transgenic mothers, as determined by Northern analysis. Although embryo transfer did not affect intrauterine growth, the pregnancy rate in transgenic mothers was markedly lower than in nontransgenic controls. In both fetal and neonatal tissues, transgene expression was significantly greater in homozygous animals when compared with heterozygotes, but the difference was magnified postnatally. These results suggest that HIV gene expression affected both mother and neonate. In the mother, expression of the HIV-1 transgene reduced postfertilization pregnancy rate, Once the animal was pregnant, however, the effects of transgene expression on the homozygous fetus were overcome in utero, possibly by the contribution of maternal factors or by inhibition of HIV-1 gene expression by a fetal or maternal factor(s). In the neonate, HIV-1 transgene expression increased dramatically in homozygotes and was associated with profound growth failure. Thus, the expression of HIV-1 and its consequences are complex and dependent on important maternal-fetal interactions. C1 NIDR,ORAL MED LAB,BETHESDA,MD 20892. NIDR,ANIM CARE UNIT,BETHESDA,MD 20892. NIDR,DEV BIOL LAB,BETHESDA,MD 20892. CHILDRENS HOSP,NATL MED CTR,CHILDRENS RES INST,WASHINGTON,DC 20010. NR 59 TC 11 Z9 11 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD JAN PY 1995 VL 37 IS 1 BP 56 EP 63 DI 10.1203/00006450-199501000-00012 PG 8 WC Pediatrics SC Pediatrics GA PY492 UT WOS:A1995PY49200010 PM 7700734 ER PT J AU HOLZMAN, C PANETH, N LITTLE, R PINTOMARTIN, J AF HOLZMAN, C PANETH, N LITTLE, R PINTOMARTIN, J TI PERINATAL BRAIN INJURY IN PREMATURE-INFANTS BORN TO MOTHERS USING ALCOHOL IN PREGNANCY SO PEDIATRICS LA English DT Article DE ALCOHOL; BRAIN; LOW BIRTH WEIGHT; HEMORRHAGE; NEONATAL; WHITE MATTER; GERMINAL MATRIX; PRENATAL; PREGNANCY ID LOW-BIRTH-WEIGHT; CEREBRAL BLOOD-FLOW; FETAL GROWTH; INTRAVENTRICULAR HEMORRHAGE; MOTOR DEVELOPMENT; MATERNAL ALCOHOL; PRENATAL ALCOHOL; CONSUMPTION; EXPOSURE; NEUROPATHOLOGY AB Objective. Alcohol has been shown to have teratogenic effects on the fetal central nervous system. However, little research has been done to assess the impact of prenatal alcohol exposure on premature infants, a group particularly vulnerable to perinatal brain injury. Methods. We examined the relation between maternal alcohol use and the most common forms of brain injury in premature infants-germinal matrix/intraventricular hemorrhage (GM/IVH) and white-matter damage-in a large population-based cohort of infants weighing 2000 g or less. The analyses included 349 infants younger than 31 weeks' gestation who received at least one cranial ultrasound scan and whose mothers were queried about prenatal alcohol use in a postpartum interview. Results. Infants of mothers who reported ''moderate'' alcohol use (fewer than seven drinks per week and fewer than three drinks per occasion) to ''high'' use (seven or more drinks per week and/or three or more drinks per occasion) before recognized pregnancy, and moderate alcohol use during pregnancy, were not at increased risk for brain injury. However, after controlling for potentially confounding factors, infants of women reporting high alcohol use during pregnancy were at increased risk of developing isolated brain hemorrhage (odds ratio [OR] = 5.5, 95% confidence interval [CI] = 1.2, 24.7), any brain hemorrhage (OR = 6.7, 95% CI = 1.8, 26.4), and white-matter damage (OR = 9.5, 95% CI = 1.9, 46.4). Conclusion. Premature infants of women who report consuming seven or more drinks per week and/or three or more drinks per occasion during pregnancy have substantially elevated risks of both of the most common forms of brain injury in premature infants. C1 NIEHS, RES TRIANGLE PK, NC 27709 USA. UNIV PENN, SCH MED, DEPT PEDIAT, PHILADELPHIA, PA 19104 USA. UNIV PENN, SCH MED, CLIN EPIDEMIOL UNIT, PHILADELPHIA, PA 19104 USA. RP HOLZMAN, C (reprint author), MICHIGAN STATE UNIV, PROGRAM EPIDEMIOL, E LANSING, MI 48824 USA. FU NIAAA NIH HHS [AA09008-02] NR 57 TC 30 Z9 30 U1 1 U2 1 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD, ELK GROVE VILLAGE, IL 60007-1098 SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1995 VL 95 IS 1 BP 66 EP 73 PG 8 WC Pediatrics SC Pediatrics GA PZ729 UT WOS:A1995PZ72900013 PM 7770312 ER PT J AU WOLTERS, PL BROUWERS, P MOSS, HA PIZZO, PA AF WOLTERS, PL BROUWERS, P MOSS, HA PIZZO, PA TI DIFFERENTIAL RECEPTIVE AND EXPRESSIVE LANGUAGE FUNCTIONING OF CHILDREN WITH SYMPTOMATIC HIV DISEASE AND RELATION TO CT SCAN BRAIN ABNORMALITIES SO PEDIATRICS LA English DT Article DE PEDIATRIC AIDS; HIV INFECTION; NEUROPSYCHOLOGY; LANGUAGE; ENCEPHALOPATHY CT SCAN ID HUMAN-IMMUNODEFICIENCY-VIRUS; INFUSION ZIDOVUDINE THERAPY; IMMUNE-DEFICIENCY SYNDROME; DEVELOPMENTAL ABNORMALITIES; PROGRESSIVE ENCEPHALOPATHY; BASAL GANGLIA; SYNDROME AIDS; INFANTS; INFECTION; GROWTH AB Objectives. To investigate the effect of HIV disease on the receptive and expressive language of children and the relationship between CT scan brain abnormalities and language functioning. Methods. Thirty-six children (mean age, 5.5 years; range, 1 through 10 years; 75% vertical transmission; 58% classified as encephalopathic) with symptomatic HIV infection and 20 uninfected siblings (mean age, 7.8 years; range, 3 through 15 years) were administered an age-appropriate comprehensive language test assessing both receptive and expressive language (Reynell Developmental Language Scales or Clinical Evaluation of Language Fundamentals-Revised). Each HIV-infected child had a CT scan of the brain as part of the baseline evaluation, which was rated independently and blindly by two neurologists, for presence and severity of brain abnormalities using a semiquantitative rating system. Results. Expressive language was significantly more impaired than receptive language in the overall sample of HIV-infected children. The encephalopathic children scored significantly lower than the non-encephalopathic children, however, the degree of discrepancy between mean receptive and expressive language scores was not significantly different between these two groups. The uninfected sibling control group did not have a significant discrepancy between receptive and expressive language, and they scored significantly higher than the infected patient group. Greater severity of CT scan abnormalities was significantly correlated with poorer receptive and expressive language functioning in the overall HIV-infected sample and a higher discrepancy between receptive and expressive language in the encephalopathic group. Conclusion. Pediatric HIV disease is associated with differential receptive and expressive language functioning in which expressive language is significantly more impaired than receptive language. The sibling data and CT scan correlations suggest that the observed language impairments are associated with the direct effects of HIV-related central nervous system disease. C1 MED ILLNESS COUNSELING CTR,CHEVY CHASE,MD. RP WOLTERS, PL (reprint author), NCI,PEDIAT BRANCH,NEUROPSYCHOL GRP,BLDG 10,13N240,10 CTR DR,MSC 1928,BETHESDA,MD 20892, USA. FU NCI NIH HHS [NCI-CM-17529-41] NR 44 TC 44 Z9 49 U1 2 U2 3 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD, ELK GROVE VILLAGE, IL 60007-1098 SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD JAN PY 1995 VL 95 IS 1 BP 112 EP 119 PG 8 WC Pediatrics SC Pediatrics GA PZ729 UT WOS:A1995PZ72900020 PM 7770287 ER PT B AU SAKAGUCHI, K SAKAMOTO, H LEWIS, MS KODAMA, H OMICHINSKI, JG GRONENBORN, AM CLORE, GM APPELLA, E AF SAKAGUCHI, K SAKAMOTO, H LEWIS, MS KODAMA, H OMICHINSKI, JG GRONENBORN, AM CLORE, GM APPELLA, E BE Ohno, M TI SOLUTION STRUCTURE OF THE HUMAN P53 OLIGOMERIZATION DOMAIN BY THE USE OF SYNTHETIC PEPTIDES AND MULTIDIMENSIONAL NMR SO PEPTIDE CHEMISTRY 1994 SE PEPTIDE CHEMISTRY LA English DT Proceedings Paper CT 32nd Symposium on Peptide Chemistry CY OCT 12-14, 1994 CL FUKUOKA WOMENS UNIV, FUKUOKA, JAPAN SP JAPANESE PEPTIDE SOC, CHEM SOC JAPAN, JAPAN SOC BIOSCI BIOTECHNOL & AGROCHEM, PHARM SOC JAPAN HO FUKUOKA WOMENS UNIV C1 NCI,BETHESDA,MD 20892. RI Sakamoto, Hiroshi/A-3181-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU PROTEIN RESEARCH FOUNDATION PI OSAKA PA 476 INA , MINOH-SHI, OSAKA 562, JAPAN BN 4-88667-132-2 J9 PEPTIDE CH PY 1995 BP 105 EP 108 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BD08Q UT WOS:A1995BD08Q00027 ER PT J AU CORREA, FMA VISWANATHAN, M CIUFFO, GM TSUTSUMI, K SAAVEDRA, JM AF CORREA, FMA VISWANATHAN, M CIUFFO, GM TSUTSUMI, K SAAVEDRA, JM TI KIDNEY ANGIOTENSIN-II RECEPTORS AND CONVERTING-ENZYME IN NEONATAL AND ADULT WISTAR-KYOTO AND SPONTANEOUSLY HYPERTENSIVE RATS SO PEPTIDES LA English DT Article DE ANGIOTENSIN II; ANGIOTENSIN-CONVERTING ENZYME; HYPERTENSION; SHR ID RENAL BLOOD-FLOW; RENIN; AUTORADIOGRAPHY; BRAIN; CAPTOPRIL; SUBTYPES; BINDING AB The aim of the present study was to correlate the development of the renin angiotensin system (RAS) in the kidney of the rat with the development of genetic hypertension. Immature (1-week-old) and adult (12-week-old) normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive kidney rats (SHR) were used for quantification of angiotensin II (ANG II) receptors and angiotensin converting enzyme (ACE) binding sites using quantitative autoradiography. In both neonatal and adult animals of either strain, ANG II receptors were of AT(l) subtype. In all kidney areas of 1-week-old rats, ANG II receptor density was higher in SHR than WKY. Binding density increased with age in WKY rats; thus, in the glomeruli and the outer stripe of the outer medulla of 12-week-old WKY, binding was significantly higher than that present in age-matched SHR. [I-125]351A binding to ACE was highest in the outer medulla and not detectable in glomeruli. In 1-week-old rats, binding to ACE was higher in WKY than in SHR strain. No differences in ACE binding were found between adult SHR and WKY rats, with the exception of the inner stripe of the outer medulla, where no binding was detected in SHR. Our results support the hypothesis that the RAS in kidney is developmentally regulated and is involved in the development and maintenance of genetic hypertension in SHR. C1 NIMH,CLIN SCI LAB,PHARMACOL SECT,BETHESDA,MD 20892. UNIV SAO PAULO,SCH MED RIBEIRAO PRETO,DEPT PHARMACOL,BR-14049 RIBEIRAO PRET,BRAZIL. RI Correa, Fernando /D-1614-2012 OI Correa, Fernando /0000-0003-4067-9524 NR 27 TC 26 Z9 26 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 1 BP 19 EP 24 DI 10.1016/0196-9781(94)00150-5 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA QD264 UT WOS:A1995QD26400004 PM 7716070 ER PT J AU DEOLIVEIRA, AM VISWANATHAN, M CAPSONI, S HEEMSKERK, FMJ CORREA, FMA SAAVEDRA, JM AF DEOLIVEIRA, AM VISWANATHAN, M CAPSONI, S HEEMSKERK, FMJ CORREA, FMA SAAVEDRA, JM TI CHARACTERIZATION OF ENDOTHELIN(A) RECEPTORS IN CEREBRAL AND PERIPHERAL ARTERIES OF THE RAT SO PEPTIDES LA English DT Article DE ENDOTHELIN RECEPTORS; CEREBRAL ARTERIES; PERIPHERAL ARTERIES ID AUTORADIOGRAPHIC LOCALIZATION; BINDING-SITES; ANTAGONIST; TISSUES; BQ-123 AB We have characterized and quantified endothelin receptors in rat brain (anterior cerebral) and peripheral (aorta, carotid, and caudal) arteries, with the use of [I-125]endothelin and quantitative autoradiography. Endothelin binding was saturable, of high affinity, and totally displaced by the selective endothelin ET(A) antagonist BQ 123. A single class of ET(A) receptors is located in the medial layer of peripheral and cerebral arteries, and its quantification by autoradiography allows study of their regulation and function. C1 NIMH,PHARMACOL SECT,CLIN SCI LAB,BETHESDA,MD 20892. RI Correa, Fernando /D-1614-2012; OI Correa, Fernando /0000-0003-4067-9524; Capsoni, Simona/0000-0003-2670-8237 NR 17 TC 15 Z9 15 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 1 BP 139 EP 144 DI 10.1016/0196-9781(94)00169-7 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA QD264 UT WOS:A1995QD26400021 PM 7716066 ER PT J AU CHA, XY XU, H RICE, KC PORRECA, F LAI, J ANANTHAN, S ROTHMAN, RB AF CHA, XY XU, H RICE, KC PORRECA, F LAI, J ANANTHAN, S ROTHMAN, RB TI OPIOID PEPTIDE RECEPTOR STUDIES .1. IDENTIFICATION OF A NOVEL DELTA-OPIOID RECEPTOR-BINDING SITE IN RAT-BRAIN MEMBRANES SO PEPTIDES LA English DT Article DE DELTA RECEPTOR; ANTISENSE DNA; DELTORPHIN; SUPERFIT; OPIOID RECEPTORS ID BETA-FUNALTREXAMINE; OPIATE RECEPTOR; HIGH-AFFINITY; DIFFERENTIAL ANTAGONISM; FUNCTIONAL EXPRESSION; MOUSE-BRAIN; NALTRINDOLE; SUBTYPES; PURIFICATION; NALTREXONE AB Our laboratory was among the first to propose the existence of delta receptor subtypes: a delta site thought to be associated with a mu-delta opioid receptor complex termed the delta(cx) binding site and delta site not associated with the mu-delta-opioid receptor complex, termed the delta(nex) site. In previous studies, we assayed the delta(cx) site with [H-3][D-Ala(2),D-Leu(5)]enkephalin using rat brain membranes depleted of delta(nex) sites by pretreatment with the site-directed acylating agent, (+)-trans-SUPERFIT. In the present study, we investigated, using (+)-trans-SUPERFIT-pretreated membranes, the possibility of heterogeneity of the delta(cx) binding site. Two sites were resolved: the delta(cx-1) site at which mu ligands are potent noncompetitive inhibitors and delta ligands are weak competitive inhibitors, and the delta(cx-2) site where delta ligands are potent and mu ligands are weak, mixed competitive-noncompetitive inhibitors. Although the delta(cx-2) site has a delta-like ligand-selectivity profile, several experiments distinguished it from the delta(nex) site. Two lines of evidence suggest that the delta(nex) site corresponds to the cloned delta receptor. One, the delta receptor was cloned from the NG108-15 cell line, and this receptor, like the delta(nex) binding site, irreversibly binds SUPERFIT and (+)-trans-SUPERFIT. Secondly, administration of delta-antisense DNA selectively decreases delta(nex) binding. Viewed collectively, the major finding of this study is the discovery of a novel SUPERFIT-insensitive and delta-antisense-insensitive delta(cx-2) binding site. C1 NIDA,IRP,CLIN PSYCHOPHARMACOL SECT,BALTIMORE,MD 21224. NIDDKD,MED CHEM LAB,BETHESDA,MD 20892. UNIV ARIZONA,COLL MED,DEPT PHARMACOL,TUCSON,AZ 85724. SO RES INST,DEPT ORGAN CHEM,BIRMINGHAM,AL 35255. NR 59 TC 20 Z9 20 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 2 BP 191 EP 198 DI 10.1016/0196-9781(94)00182-0 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA QM363 UT WOS:A1995QM36300003 PM 7784248 ER PT J AU DRAOUI, M CHUNG, P PARK, M BIRRER, M JAKOWLEW, S MOODY, TW AF DRAOUI, M CHUNG, P PARK, M BIRRER, M JAKOWLEW, S MOODY, TW TI BOMBESIN STIMULATES C-FOS AND C-JUN MESSENGER-RNAS IN SMALL-CELL LUNG-CANCER CELLS SO PEPTIDES LA English DT Article DE SMALL CELL LUNG CANCER (SCLC); GRP RECEPTOR; C-FOS; C-JUN ID GASTRIN-RELEASING PEPTIDE; SWISS 3T3 CELLS; CYTOSOLIC CALCIUM; HIGH-AFFINITY; CARCINOMA; GROWTH; RECEPTOR; MYC; ANTAGONISTS; CLONING AB The effects of bombesin/gastrin-releasing peptide (BN/GRP) on c-fos and c-jun gene expression were investigated using small cell lung cancer (SCLC) cells. BN(10 nM) increased c-fos mRNA fivefold using NCI-H345 or NCI-HS 10 cells. The increase was concentration dependent with 1 nM BN half-maximally increasing c-fos mRNA. Also, the increase in c-fos mRNA caused by BN was time dependent, being maximal after 1 h and returning to basal values after 4 h. GRP and GRP(14-27) but not GRP(1-16) increased c-fos mRNA. BW2258U89 (1 mu M), a GRP receptor antagonist, had no effect on basal c-fos but inhibited the increase in c-fos mRNA caused by 10 nM BN. Also, BN transiently increased c-jun mRNA twofold and the increase caused by BN was blocked by BW2258U89. These data suggest that GRP receptors may regulate nuclear oncogene gene expression in SCLC cells. C1 NCI,DCPC,BIOMARKERS & PREVENT RES BRANCH,ROCKVILLE,MD 20850. GEORGE WASHINGTON UNIV,MED CTR,DEPT MOLEC BIOL & BIOCHEM,WASHINGTON,DC 20037. FU NCI NIH HHS [CA53477] NR 28 TC 39 Z9 39 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 2 BP 289 EP 292 DI 10.1016/0196-9781(94)00173-1 PG 4 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA QM363 UT WOS:A1995QM36300017 PM 7784258 ER PT J AU FELLEY, CP LIN, JT MANTEY, SA PRADHAN, TK BENYA, RV JENSEN, RT AF FELLEY, CP LIN, JT MANTEY, SA PRADHAN, TK BENYA, RV JENSEN, RT TI BOMBESIN DOES NOT STIMULATE PEPSINOGEN RELEASE IN ISOLATED GASTRIC CHIEF CELLS SO PEPTIDES LA English DT Article DE CYTOSOLIC CALCIUM; CYCLIC AMP; INOSITOL PHOSPHATES; GUINEA PIG ID PANCREATIC ACINAR-CELLS; GUINEA-PIG STOMACH; SWISS 3T3 CELLS; RECEPTOR ANTAGONISTS; SIGNAL-TRANSDUCTION; DISPERSED GLANDS; RAT STOMACH; SECRETION; PEPTIDE; BINDING AB Bombesin (BN)-related peptides, such as gastrin-releasing peptide (GRP), have been shown in vivo to stimulate release of pepsinogen. However, whether this is due to a direct interaction with chief cells is not clear. To clarify this we prepared isolated chief cells (>90% pure) from guinea pig stomach. BN, GRP, or neuromedin B (NMB), at concentrations up to 1 mu M, did not stimulate pepsinogen release or affect the stimulation caused by vasoactive intestinal peptide (VIP) (100 nM) or CCK-8 (10 nM), respectively. In addition, BN, GRP, or NMB at a concentration of 1 mu M did not increase cAMP nor did they alter the increase in cAMP caused by VIP or secretin. BN (1 mu M) did not alter basal cytosolic calcium [Ca2+](i) or affect the increase in [Ca2+](i) caused by CCK-8 (1 mu M). Furthermore, BN, GRP, or NMB at a concentration of 1 mu M did not increase the generation of inositol phosphates (IF) or alter the increase in [H-3]IP1, [H-3]IP2, or [H-3]IP3, caused by CCK-8 (1 mu M) or carbachol (1 mM). Binding studies demonstrated no saturable binding of either [I-125][Tyr(4)]BN or [I-125][D-Tyr(0)]NMB using experimental conditions where binding with other peptide ligands to other receptors on chief cells is seen. We conclude that BN-related peptides do not interact directly with specific receptors on chief cells to stimulate or alter stimulated pepsinogen secretion, increase the breakdown of inositol phosphates, or alter [Ca2+](i) or cAMP. C1 NIDDKD,DIGEST DIS BRANCH,BETHESDA,MD 20892. NR 53 TC 3 Z9 3 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 3 BP 383 EP 388 DI 10.1016/0196-9781(94)00208-N PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA QV892 UT WOS:A1995QV89200003 PM 7651888 ER PT J AU NI, Q XU, H PARTILLA, JS DECOSTA, BR RICE, KC KAYAKIRI, H ROTHMAN, RB AF NI, Q XU, H PARTILLA, JS DECOSTA, BR RICE, KC KAYAKIRI, H ROTHMAN, RB TI OPIOID PEPTIDE RECEPTOR STUDIES .3. INTERACTION OF OPIOID-PEPTIDES AND OTHER DRUGS WITH 4 SUBTYPES OF THE KAPPA(2) RECEPTOR IN GUINEA-PIG BRAIN SO PEPTIDES LA English DT Article DE OPIOID PEPTIDES; KAPPA RECEPTOR; GUINEA PIG BRAIN; BINDING SITES ID BOVINE ADRENAL-MEDULLA; BINDING-SITES; RAT-BRAIN; OPIATE RECEPTORS; DIFFERENTIAL ANTAGONISM; BETA-FUNALTREXAMINE; HIGH-AFFINITY; AGONISTS; DYNORPHIN; LIGANDS AB Using guinea pig, rat, and human brain membranes depleted of mu and delta receptors by pretreatment with the site-directed acylating agents BIT (mu selective) and FIT (delta selective), previous studies from our laboratory resolved two subtypes of the kappa(2) binding site, termed kappa(2a) and kappa(2b). In more recent studies, we used 6 beta-[(125)Iodo]-3,14-dihydroxy-17-cyclopropyylmethyl-4,5 alpha-epoxymorphinan ([I-125]IOXY) to characterize multiple kappa(2) binding sites in rat brain. The results indicated that [I-125]IOXY, like [H-3]bremazocine, selectively labels kappa(2) binding sites in rat brain membranes pretreated with BIT and FIT. In the rat brain, using 100 nM [D-Ala(2)-MePhe(4),Gly-ol(5)] enkephalin to block [I-125]IOXY binding to the kappa(2b) site, we resolved two subtypes of the kappa(2a) binding site. In the present study we examined the binding of [I-125]IOXY to the kappa(2) receptors of guinea pig brain. As observed in rat brain, [I-125]IOXY, under appropriate assay conditions, selectively labels kappa(2) binding sites. Quantitative binding studies readily demonstrated the presence of kappa(2a) and kappa(2h) binding sites. The kappa(2a) binding sites were selectively assayed using 5 mu M [Leu(5)]enkephalin to block [I-125]IOXY binding to the kappa(2a) sites, and kappa(2b) sites were selectively assayed using 5 mu M (-)-(1S,2S)-U50,488 to block [I-125]IOXY binding to the kappa(2a) sites. Under these conditions, two subtypes of the kappa(2a) site were resolved with high (kappa(2a-1)) and low (kappa(2a-2)) affinity for nor-BNI (K-i values = 0.88 and 476 nM) and CI977 (K-i values = 17.5 and 95,098 nM). Similarly, two subtypes of the kappa(2b) site were observed with high (kappa(2h-1)) and low (kappa(2b-2)) affinity for [D-Ala(2)-MePhe(4),Gly-ol(5)]enkephalin (DAMGO) (K-i values = 97 and 12,321 nM) and alpha-neoendorphin (K-i values = 33 and 5308 nM). Two-site models were also resolved in the presence of 100 mu M 5'-guanylyimidodiphosphate (GppNHp). We carried out detailed ligand selectivity analysis of the multiple kappa(2) binding sites. Most test agents were either nonselective or selective for the kappa(2a-2) site. Nalbuphine was moderately selective for the kappa(2a-2) site. Similarly, although most test agents were either nonselective or selective for the kappa(2b-1) site, butorphanol, and the delta antagonists naltrindole, naltriben, and 7-benzylidene-7-dehydronaltrexone were moderately selective for the kappa(2b-2) site. Of the endogenous opioid peptides tested, BAM22P had the highest affinity for the kappa(2b-2). Site (31 nM) and peptide E had the highest affinity for the kappa(2a-2) binding site (53 nM). These data provide additional evidence for heterogeneity of the kappa-opioid receptor and new targets for drug design, synthesis, and therapeutics. C1 NIDA,DIR,CPS,BALTIMORE,MD 21224. NIDDK,MED CHEM LAB,BETHESDA,MD 20892. NR 64 TC 11 Z9 11 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 6 BP 1083 EP 1095 DI 10.1016/0196-9781(95)00091-W PG 13 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA RU938 UT WOS:A1995RU93800015 PM 8532593 ER PT J AU KNIGHT, M TAKAHASHI, K CHANDRASEKHAR, B GEBLAOUI, AZ JENSEN, RT STRADER, D MOODY, TW AF KNIGHT, M TAKAHASHI, K CHANDRASEKHAR, B GEBLAOUI, AZ JENSEN, RT STRADER, D MOODY, TW TI INHIBITORY CYCLIC ANALOGS AND CHLORAMBUCIL DERIVATIVES OF BOMBESIN-LIKE PEPTIDES SO PEPTIDES LA English DT Article DE BOMBESIN; GRP; BN RECEPTORS; ANTAGONISTS; INHIBITORS; CHLORAMBUCIL PEPTIDES; ALKYLATING ANALOGS; NITROGEN MUSTARD; CYCLIC PEPTIDES; SCLC; PANCREATIC ACINAR CELLS ID CELL LUNG-CANCER; SWISS 3T3 CELLS; AUTOCRINE GROWTH-FACTORS; GUINEA-PIG PANCREAS; RECEPTOR ANTAGONISTS; CYTOSOLIC CALCIUM; DISPERSED ACINI; HIGH-AFFINITY; POTENT; CLONING AB Analogues of the amphibian neuropeptide, bombesin, and of the mammalian homologue, gastrin-releasing peptide, have been synthesized and their biological activity studied in small cell lung carcinoma and rat pancreatic acinar cells. The compounds are truncated sequences of the active tetradecapeptide BN(1-14) or GRP(20-27). Peptides were cyclized between position 5 or 7 and the carboxyl end of the des-Met(14) fragment with D and L Ala(11) and Lys(5) substitutions, as well as various N-terminal groups attached. The smallest cyclic peptide, BN(7-13), bound to SCLC membranes with mu M potency and inhibited BN stimulation of intracellular Ca(++) levels. The most potent inhibitor is N-chlorambucil-[His(7),D-Ala(11)]BN(7-13)ethyl ester, which antagonized BN function in SCLC and acinar cells with nM potency and also inhibited clonal growth of carcinoma cell lines. C1 NIDDK, DIGEST DIS BRANCH, BETHESDA, MD 20892 USA. GEORGE WASHINGTON UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOL, WASHINGTON, DC 20037 USA. RP KNIGHT, M (reprint author), PEPTIDE TECHNOL CORP, 8401 HELGERMAN COURT, GAITHERSBURG, MD 20877 USA. OI Knight, Martha/0000-0003-4863-8858 FU NCI NIH HHS [CA 55468, CA 53477] NR 38 TC 7 Z9 7 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 6 BP 1109 EP 1115 DI 10.1016/0196-9781(95)00074-T PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA RU938 UT WOS:A1995RU93800017 PM 8532595 ER PT J AU MOODY, TW FAGARASAN, M ZIA, F AF MOODY, TW FAGARASAN, M ZIA, F TI NEUROMEDIN-B STIMULATES ARACHIDONIC-ACID RELEASE, C-FOS GENE-EXPRESSION, AND THE GROWTH OF C6 GLIOMA-CELLS SO PEPTIDES LA English DT Article DE NEUROMEDIN B; RECEPTOR BINDING; ARACHIDONIC ACID; C-FOS; GROWTH; CYTOSOLIC CALCIUM ID SWISS 3T3 CELLS; BOMBESIN-LIKE PEPTIDE; MOLECULAR-CLONING; HIGH-AFFINITY; RAT-BRAIN; RECEPTOR; LOCALIZATION; ANTAGONIST; PITUITARY; ANALOGS AB The effects of neuromedin B (NMB) on C6 glioma cells were investigated. NMB bound with high affinity (IC50 = 1 nM) to C6 cells whereas BN and GRP were less potent (IC50 = 40 and 100 nM). NMB (1 nM) elevated cytosolic Ca2+ in individual C6 cells and the increase in cytosolic Ca2+ was reversed by 1 mu M [D-Arg(1),D-Pro(2),D-Trp(7,9),Leu(11)]substance P [APTTL]SP, a broad spectrum antagonist. NMB stimulated [H-3]arachidonic acid release from C6 cells and the increase in [H-3]arachidonic acid release was reversed [APTTL]SP. NMB increased transiently c-fos gene expression in C6 cells. NMB increased the number of C6 colonies in soft a,oar and the increase in growth caused by NMB was reversed by [APTTL]SP. These data suggest that NMB receptors may regulate the proliferation of C6 cells. C1 GEORGE WASHINGTON UNIV,SCH MED & HLTH SCI,DEPT MICROBIOL,WASHINGTON,DC 20037. GEORGE WASHINGTON UNIV,SCH MED & HLTH SCI,DEPT BIOCHEM,WASHINGTON,DC 20037. GEORGE WASHINGTON UNIV,SCH MED & HLTH SCI,DEPT MOLEC BIOL,WASHINGTON,DC 20037. RP MOODY, TW (reprint author), NCI,BPRB,BLDG C,RM 300,9610 MED CTR DR,ROCKVILLE,MD 20850, USA. NR 32 TC 14 Z9 14 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 6 BP 1133 EP 1140 DI 10.1016/0196-9781(95)00085-X PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA RU938 UT WOS:A1995RU93800020 PM 8532598 ER PT J AU VGONTZAS, AN FRIEDMAN, TC CHROUSOS, GP BIXLER, EO VELABUENO, A KALES, A AF VGONTZAS, AN FRIEDMAN, TC CHROUSOS, GP BIXLER, EO VELABUENO, A KALES, A TI DELTA-SLEEP-INDUCING PEPTIDE IN NORMAL HUMANS AND IN PATIENTS WITH SLEEP-APNEA AND NARCOLEPSY SO PEPTIDES LA English DT Article DE DSIP; SLEEP APNEA; NARCOLEPSY; SLOW-WAVE SLEEP ID PLASMA-LEVELS; DSIP; RADIOIMMUNOASSAY; IMMUNOREACTIVITY; RATS AB We measured morning plasma concentrations of delta sleep-inducing-peptide-like-immunoreactivity (DSIP-LI) in 9 sleep apnea patients, 10 narcolepsy patients, and 11 normal controls. Comparisons between the three groups showed no significant differences, although there was a trend toward association with low levels of DSIP-LI in the narcoleptic group, particularly in patients not using medications. No differences were found in the morning or evening plasma DSIP-LI levels in a second group of 11 normal controls and 8 sleep apneics. Our findings do not appear to support a biological marker role of disease activity for single measures of plasma DSIP in sleep apnea. C1 NIH,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP VGONTZAS, AN (reprint author), PENN STATE UNIV,DEPT PSYCHIAT,CTR SLEEP RES & TREATMENT,500 UNIV DR,HERSHEY,PA 17033, USA. NR 23 TC 2 Z9 2 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0196-9781 J9 PEPTIDES JI Peptides PY 1995 VL 16 IS 6 BP 1153 EP 1156 DI 10.1016/0196-9781(95)00092-X PG 4 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA RU938 UT WOS:A1995RU93800023 PM 8532601 ER PT B AU Johnson, R AF Johnson, R BE Karmos, G Molnar, M Csepe, V Czigler, I Desmedt, JE TI On the relation between exogenous and endogenous ERP component activity: Evidence from patients with a subcortical dementia SO PERSPECTIVES OF EVENT-RELATED POTENTIALS RESEARCH SE ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY SUPPLEMENT LA English DT Proceedings Paper CT 10th International Conference on Event-Related Potentials of the Brain (EPIC X) CY JUN, 1992 CL EGER, HUNGARY C1 NINCDS,COGNIT NEUROPHYSIOL UNIT,COGNIT NEUROSCI SECT,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL B V PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS BN 0-444-82058-2 J9 EEG CL N SU PY 1995 IS 44 BP 414 EP 427 PG 14 WC Neurosciences SC Neurosciences & Neurology GA BF88H UT WOS:A1995BF88H00044 ER PT J AU NELSON, PG FIELDS, RD LIU, Y AF NELSON, PG FIELDS, RD LIU, Y TI NEURAL ACTIVITY, NEURON-GLIA RELATIONSHIPS, AND SYNAPSE DEVELOPMENT SO PERSPECTIVES ON DEVELOPMENTAL NEUROBIOLOGY LA English DT Article DE SYNAPSE ELIMINATION; ELECTRICAL ACTIVITY; INTRACELLULAR CALCIUM; NEUROGLIA; PROTEASE; NEXIN I; VASOACTIVE INTESTINAL PEPTIDE ID POSTNATAL-DEVELOPMENT; PROTEASE INHIBITORS; CHICK-EMBRYO; SPINAL-CORD; NEUROMUSCULAR-JUNCTION; ARACHIDONIC-ACID; NERVOUS-SYSTEM; VISUAL-CORTEX; MOTOR NEURONS; LOW CALCIUM AB There is considerable evidence for elimination of synapses and loss of neurons during development of the nervous system. Electrical activity in developing neural circuits induces functional and structural refinement of many synaptic connections, but it is unclear whether the fundamental mechanism is one of strengthening appropriate synapses, combined with the regression of synapses that fail to become adequately stabilized, versus a mechanism of elimination that specifically acts on inappropriate connections. A model of selective synapse elimination, based on the activity-dependent release of proteases and glial-derived protease inhibitors, is presented and supported by evidence from an in vitro preparation of the mouse neuromuscular junction. RP NELSON, PG (reprint author), NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892, USA. NR 69 TC 25 Z9 25 U1 0 U2 0 PU GORDON BREACH SCI PUBL LTD PI READING PA C/O STBS LTD PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1064-0517 J9 PERSPECT DEV NEUROBI JI Perspect. Dev. Neurobiol. PY 1995 VL 2 IS 4 BP 399 EP 407 PG 9 WC Developmental Biology; Neurosciences SC Developmental Biology; Neurosciences & Neurology GA QK106 UT WOS:A1995QK10600012 PM 7757409 ER PT J AU CAPORASO, N DEBAUN, MR ROTHMAN, N AF CAPORASO, N DEBAUN, MR ROTHMAN, N TI LUNG-CANCER AND CYP2D6 (THE DEBRISOQUINE POLYMORPHISM) - SOURCES OF HETEROGENEITY IN THE PROPOSED ASSOCIATION SO PHARMACOGENETICS LA English DT Article ID HYDROXYLATION PHENOTYPE; GENETIC SUSCEPTIBILITY; METABOLIC PHENOTYPE; POOR METABOLIZERS; CIGARETTE-SMOKING; OXIDATION; RISK; POPULATION; FAMILY AB Lung cancer association studies have yielded suggestive but not definitive results for a few genes: CYP1A1 (in Japanese), GSTM1 and CYP2D6. We focus on variability in studies of lung cancer and the CYP2D6 gene (debrisoquine metabolic phenotype) as an instructive case and we propose some sources for this heterogeneity, Beyond the general sources of bias in all field studies, three specific concerns are relevant. First, evidence that CYP2D6 is expressed in the brain. The metabolic phenotypes have distinct psychological profiles and therefore there is the potential to distort studies through selection bias. Second, among the lung cancer histologic types, adenocarcinoma likely does not share increased genetic susceptibility due to CYP2D6. Third, the degree of smoking is likely to be related to genetic susceptibility. Effect modification by smoking should be sought for any putative genetic marker for lung cancer. Progress in understanding genetic susceptibility is likely to depend on Future well-designed studies that adjust for these and other sources of bias. We are currently reanalyzing the original data from the published studies in order to further explore these issues. C1 NCI,EPIDEMIOL & BIOSTAT PROGRAM,ENVIRONM EPIDEMIOL BRANCH,ROCKVILLE,MD 20892. RP CAPORASO, N (reprint author), NCI,EPIDEMIOL & BIOSTAT PROGRAM,GENET EPIDEMIOL BRANCH,EXECUT PL N 439,6130 EXECUT BLVD,ROCKVILLE,MD 20892, USA. NR 43 TC 28 Z9 28 U1 0 U2 0 PU CHAPMAN HALL LTD PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PY 1995 VL 5 SI SI BP S129 EP S134 DI 10.1097/00008571-199512001-00014 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA RE183 UT WOS:A1995RE18300014 PM 7581482 ER PT J AU FERNANDEZSALGUERO, P GONZALEZ, FJ AF FERNANDEZSALGUERO, P GONZALEZ, FJ TI THE CYP 2A GENE SUBFAMILY - SPECIES-DIFFERENCES, REGULATION, CATALYTIC ACTIVITIES AND ROLE IN CHEMICAL CARCINOGENESIS SO PHARMACOGENETICS LA English DT Article ID HUMAN-LIVER-MICROSOMES; RABBIT NASAL MICROSOMES; COUMARIN 7-HYDROXYLASE ACTIVITY; CDNA-DIRECTED EXPRESSION; HYDROXYLASE-ACTIVITY; MOUSE P450COH; CYTOCHROME-P-450; PURIFICATION; MICE; IDENTIFICATION AB The CYP2A subfamily has been characterized in several mammalian species including mouse, rat, rabbit, hamster, cattle and human, Marked species differences have been demonstrated in the catalytic activities and regulation of this subfamily, In humans, the CYP2A genes are found as a cluster on the long arm of chromosome 19 with the CYP2B and CYP2F genes. Marked interindividual differences in expression of the CYP2A6 gene was found in livers analyzed in vitro and in humans phenotyped in vivo by using coumarin, a specific substrate for the enzyme. Efforts are underway to determine the existence of mutant and variant CYP2A6 alleles in the human population, Since CYP2A6 is able to metabolically-activate chemical carcinogens and is expressed in extrahepatic tissue, it will be of interest to determine whether genetic differences in expression of the gene is associated with cancer risk. C1 NCI,BETHESDA,MD 20892. OI Fernandez-Salguero, Pedro M./0000-0003-2839-5027 NR 56 TC 54 Z9 58 U1 0 U2 2 PU CHAPMAN HALL LTD PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PY 1995 VL 5 SI SI BP S123 EP S128 DI 10.1097/00008571-199512001-00013 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA RE183 UT WOS:A1995RE18300013 PM 7581481 ER PT J AU KATO, S ONDA, M MATSUKURA, N TOKUNAGA, A TAJIRI, T KIM, DY TSURUTA, H MATSUDA, N YAMASHITA, K SHIELDS, PG AF KATO, S ONDA, M MATSUKURA, N TOKUNAGA, A TAJIRI, T KIM, DY TSURUTA, H MATSUDA, N YAMASHITA, K SHIELDS, PG TI CYTOCHROME P4502E1 (CYP2E1) GENETIC-POLYMORPHISM IN A CASE-CONTROL STUDY OF GASTRIC-CANCER AND LIVER-DISEASE SO PHARMACOGENETICS LA English DT Article ID OXIDATION; SENSITIVITY; ETHANOL AB Cytochrome P4502E1 (CYP2E1) activates carcinogenic N-nitrosamines, benzene, urethane and other low molecular weight compounds, This enzyme is also inducible by ethanol, and metabolizes alcohol. A restriction fragment length polymorphism (RFLP) using the Rsa I restriction enzyme has been identified in the CYP2E1 transcription regulatory region; recent studies suggest that this polymorphism may affect gene expression. We investigated the frequency of the Rsa I RFLP in a Japanese population in relation to gastric cancer and liver disease susceptibility. The frequency of this polymorphism was determined in 150 gastric cancer, 16 hepatocellular cancer, 48 liver cirrhosis and 203 benign gastric disease (controls) patients. This preliminary study shows no association of the specific genotype with gastric cancer in all subjects (odds ratio = 1.04, 95% CI = 0.74-3.08 for the heterozygote and 0.57, 95% CI = 0.22-1.50 for the homozygous rare allele, respectively), To further confirm this lack of association, an age and gender matched case-control study should be performed. Separately, there was no association of the Rsa I RFLP with hepatocellular carcinoma (p = 0.911), but there was a suggested difference between the non-viral associated liver cirrhosis patients and control patients. Thus, this polymorphism may be related to ethanol metabolism and consequential liver diseases in a Japanese population. C1 NCI,DIV CANC ETIOL,HUMAN CARCINOGENESIS LAB,BETHESDA,MD 20892. RP KATO, S (reprint author), NIPPON MED COLL,DEPT SURG 1,BUNKYO KU,1-1-5 SENDAGI,TOKYO 113,JAPAN. RI Shields, Peter/I-1644-2012 NR 20 TC 31 Z9 32 U1 0 U2 2 PU CHAPMAN HALL LTD PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PY 1995 VL 5 SI SI BP S141 EP S144 DI 10.1097/00008571-199512001-00016 PG 4 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA RE183 UT WOS:A1995RE18300016 PM 7581484 ER PT J AU BUTSCHKY, MF BAILEY, D HENNINGFIELD, JE PICKWORTH, WB AF BUTSCHKY, MF BAILEY, D HENNINGFIELD, JE PICKWORTH, WB TI SMOKING WITHOUT NICOTINE DELIVERY DECREASES WITHDRAWAL IN 12-HOUR ABSTINENT SMOKERS SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE DE-NICOTINIZED CIGARETTE; TOBACCO WITHDRAWAL; SENSORY FACTORS; SMOKING BEHAVIOR ID CIGARETTE-SMOKING; TOBACCO WITHDRAWAL; CESSATION; TAR AB The contribution of sensory factors to smoking satisfaction and nicotine withdrawal symptoms was assessed by evaluating responses to three types of cigarettes: a regular cigarette, a de-nicotinized cigarette (de-nic), and a lettuce leaf cigarette. Doses were varied by requiring subjects to smoke cigarettes using a five-port cigarette manifold. The ratio of the regular or de-nic cigarettes to the lettuce cigarettes was varied across the following values: zero, one, two, and four of five. Seven male smokers were tobacco-deprived for 12 h before testing. On one test day they smoked the de-nic cigarettes, and on another day they smoked the regular cigarettes. Ratings of satisfaction and cigarette liking were directly related to the number of regular or de-nic cigarettes, but were generally higher after the regular cigarette. The regular and de-nic cigarettes were equivalent in reducing acute withdrawal symptoms. Expired CO was similar on both experimental days. The regular cigarette dose-dependently increased plasma nicotine, but the de-nic cigarette did not increase plasma nicotine. These results indicate that sensory characteristics of cigarettes contribute to the abuse liability of smoke-delivered nicotine. The results suggest that smoking cigarettes that do not provide nicotine may temporarily suppress cigarette withdrawal symptoms. C1 NIDA,ADDICT RES CTR,BALTIMORE,MD 21224. NR 25 TC 89 Z9 89 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD JAN PY 1995 VL 50 IS 1 BP 91 EP 96 DI 10.1016/0091-3057(94)00269-O PG 6 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA QA547 UT WOS:A1995QA54700014 PM 7700960 ER PT J AU CURTI, BD SMITH, JW AF CURTI, BD SMITH, JW TI INTERLEUKIN-1 IN THE TREATMENT OF CANCER SO PHARMACOLOGY & THERAPEUTICS LA English DT Review DE INTERLEUKIN-1; HEMATOPOIESIS; MALIGNANCY; CYTOKINES; GRANULOPOIESIS; THROMBOPOIESIS ID COLONY-STIMULATING FACTOR; TUMOR NECROSIS FACTOR; RECOMBINANT HUMAN INTERLEUKIN-1-ALPHA; PHASE-I TRIAL; SYNGENEIC TUMORS; BEARING MICE; CELLS; IL-1; COMBINATION; CARBOPLATIN AB Interleukin-1 (IL-1) is a cytokine with many activities central to immune function and hematopoiesis. Many IL-1 properties can be potentially exploited in the treatment of malignancy. This review describes the toxicities and antitumor effects observed in Phase I and II trials of IL-1 in cancer patients. The immunophysiology and the induction of cytokines by IL-1 has been examined in many of the Phase I trials and has aided in understanding IL-1 effects in humans. The influence of IL-1 on granulopoiesis and thrombopoiesis when given by different regimes is also reviewed in detail. RP CURTI, BD (reprint author), NCI,FREDERICK CANC RES FACIL,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701, USA. NR 61 TC 14 Z9 14 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0163-7258 J9 PHARMACOL THERAPEUT JI Pharmacol. Ther. PY 1995 VL 65 IS 3 BP 291 EP 302 DI 10.1016/0163-7258(95)98595-H PG 12 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QR859 UT WOS:A1995QR85900001 PM 7644565 ER PT J AU WEISS, GH GITTERMAN, M AF WEISS, GH GITTERMAN, M TI EXPANSION OF MOMENTS OF THE SMOLUCHOWSKI EQUATION SO PHYSICAL REVIEW E LA English DT Article ID DIFFUSION-CONTROLLED REACTIONS; PROBABILITY C1 BAR ILAN UNIV,DEPT PHYS,IL-52100 RAMAT GAN,ISRAEL. RP WEISS, GH (reprint author), NIH,DIV COMP RES & TECHNOL,PHYS SCI LAB,BETHESDA,MD 20892, USA. NR 15 TC 11 Z9 11 U1 1 U2 2 PU AMERICAN PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 1063-651X J9 PHYS REV E JI Phys. Rev. E PD JAN PY 1995 VL 51 IS 1 BP 122 EP 125 DI 10.1103/PhysRevE.51.122 PG 4 WC Physics, Fluids & Plasmas; Physics, Mathematical SC Physics GA QD151 UT WOS:A1995QD15100017 ER PT J AU PODGORNIK, R SVETINA, S ZEKS, B AF PODGORNIK, R SVETINA, S ZEKS, B TI PARAMETRIZATION INVARIANCE AND SHAPE EQUATIONS OF ELASTIC AXISYMMETRICAL VESICLES SO PHYSICAL REVIEW E LA English DT Article ID CURVATURE C1 UNIV LJUBLJANA,FAC MED,INST BIOPHYS,LJUBLJANA 61105,SLOVENIA. UNIV LJUBLJANA,JOZEF STEFAN INST,LJUBLJANA 61105,SLOVENIA. JOZEF STEFAN INST,DEPT THEORET PHYS,LJUBLJANA 61111,SLOVENIA. RP PODGORNIK, R (reprint author), NIH,DIV COMP RES & TECHNOL,STRUCT BIOL LAB,BETHESDA,MD 20892, USA. RI Podgornik, Rudolf/C-6209-2008 OI Podgornik, Rudolf/0000-0002-3855-4637 NR 9 TC 14 Z9 14 U1 0 U2 4 PU AMERICAN PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 1063-651X J9 PHYS REV E JI Phys. Rev. E PD JAN PY 1995 VL 51 IS 1 BP 544 EP 547 DI 10.1103/PhysRevE.51.544 PG 4 WC Physics, Fluids & Plasmas; Physics, Mathematical SC Physics GA QD151 UT WOS:A1995QD15100066 ER PT J AU SICARDROSENBAUM, L LORD, D DANOFF, JV THOM, AK ECKHAUS, MA AF SICARDROSENBAUM, L LORD, D DANOFF, JV THOM, AK ECKHAUS, MA TI EFFECTS OF CONTINUOUS THERAPEUTIC ULTRASOUND ON GROWTH AND METASTASIS OF SUBCUTANEOUS MURINE TUMORS SO PHYSICAL THERAPY LA English DT Article DE METHYLCHOLANTHRENE; NEOPLASM; TUMOR; ULTRASOUND ID CANCER-THERAPY; HYPERTHERMIA; PROLIFERATION AB Background and Purpose. The use of therapeutic ultrasound (US) in the presence of malignant neoplasms has been contraindicated in physical therapy practice despite a lack of convincing scientific evidence. Some studies have shown that high levels of US, which increase tissue temperatures greater than 42-degrees-C, can kill tumors. We sought to determine whether the application of continuous therapeutic US would alter the growth or metastasis of methylcholanthrene-induced solid tumors in mice. Subjects. Seventy-one female C57BL/6 mice, age 6 to 8 weeks, received subcutaneous injections of 5 x 10(5) tumor cells. Methods. When tumors grew to 0.5 cm in diameter, the mice were randomly assigned to either a control group (n=34) or an experimental group (n=37). The experimental group received 10 treatments over a 2-week period of 3-MHz continuous US at 1.0 W/cm2 for 5 minutes, using a 0.5-cm2 sound head directly over the tumor. The control group received the same handling except for the US treatment. Tumor dimensions were measured on days 1 (baseline), 7 (midtreatment), and 14 (preexcision and postexcision). Tumors were weighted after excision, and the mice were evaluated by necropsy and histopathology of regional lymph nodes. Results. All tumors grew larger over time, but final tumor volume and weight were larger in the experimental group (789 mm3 and 0.932 g) than in the control group (395 mm3 and 0.506 g). No significant difference existed in the number of metastatic lymph nodes between groups. Conclusion and Discussion. Continuous therapeutic US increased the volume and weight of subcutaneous murine tumors in mice. We urge caution in the use of continuous therapeutic US in the areas of tumors or suspected tumors. C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT REHABIL MED,PHYS THERAPY SECT,BETHESDA,MD 20892. WILSON HLTH CARE CTR,GAITHERSBURG,MD 20852. HOWARD UNIV,COLL ALLIED HLTH,DEPT PHYS THERAPY,WASHINGTON,DC 20059. NCI,SURG BRANCH,BETHESDA,MD 20892. NCI,VETERINARIAN RESOURCES PROGRAM,SCI SERV BRANCH,LAB SCI SECT,BETHESDA,MD 20892. NR 30 TC 11 Z9 11 U1 1 U2 2 PU AMER PHYS THER ASSN PI ALEXANDRIA PA 1111 N FAIRFAX ST, ALEXANDRIA, VA 22314 SN 0031-9023 J9 PHYS THER JI Phys. Ther. PD JAN PY 1995 VL 75 IS 1 BP 3 EP 11 PG 9 WC Orthopedics; Rehabilitation SC Orthopedics; Rehabilitation GA QB033 UT WOS:A1995QB03300002 PM 7809195 ER PT J AU SICARDROSENBAUM, L LORD, D DANOFF, JV THOM, AK ECKHAUS, MA AF SICARDROSENBAUM, L LORD, D DANOFF, JV THOM, AK ECKHAUS, MA TI EFFECTS OF CONTINUOUS THERAPEUTIC ULTRASOUND ON GROWTH AND METASTASIS OF SUBCUTANEOUS MURINE TUMORS - AUTHOR RESPONSE SO PHYSICAL THERAPY LA English DT Letter C1 NIH,WARREN GRANT MAGNUSON CLIN CTR,DEPT REHABIL MED,PHYS THERAPY SECT,BETHESDA,MD 20892. WILSON HLTH CARE CTR,GAITHERSBURG,MD 20852. HOWARD UNIV,COLL ALLIED HLTH,DEPT PHYS THERAPY,WASHINGTON,DC 20059. NCI,SURG BRANCH,BETHESDA,MD 20892. NCI,VETERINARIAN RESOURCES PROGRAM,SCI SERV BRANCH,LAB SCI SECT,BETHESDA,MD 20892. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYS THER ASSN PI ALEXANDRIA PA 1111 N FAIRFAX ST, ALEXANDRIA, VA 22314 SN 0031-9023 J9 PHYS THER JI Phys. Ther. PD JAN PY 1995 VL 75 IS 1 BP 13 EP 13 PG 1 WC Orthopedics; Rehabilitation SC Orthopedics; Rehabilitation GA QB033 UT WOS:A1995QB03300004 ER PT J AU PARSEGIAN, VA SHIH, AS AF PARSEGIAN, VA SHIH, AS TI ATOM-PLANE EXPT LIVED UP TO ITS (R(-3)) POTENTIAL SO PHYSICS TODAY LA English DT Letter C1 USN,RES LAB,WASHINGTON,DC 20375. RP PARSEGIAN, VA (reprint author), NIH,BLDG 10,BETHESDA,MD 20892, USA. NR 3 TC 0 Z9 0 U1 1 U2 2 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0031-9228 J9 PHYS TODAY JI Phys. Today PD JAN PY 1995 VL 48 IS 1 BP 75 EP 75 PG 1 WC Physics, Multidisciplinary SC Physics GA QA394 UT WOS:A1995QA39400031 ER PT J AU WEIFFENBACH, JM SCHWARTZ, LK ATKINSON, JC FOX, PC AF WEIFFENBACH, JM SCHWARTZ, LK ATKINSON, JC FOX, PC TI TASTE PERFORMANCE IN SJOGRENS-SYNDROME SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE TASTE THRESHOLD; TASTE INTENSITY; TASTE DEFICITS; TASTE COMPLAINTS; SALIVARY FLOW; SJOGRENS SYNDROME; WOMEN; SALIVARY GLAND DYSFUNCTION ID SMELL AB Sjogren's Syndrome (SS) patients have impaired salivary gland function and an elevated frequency of oral complaints. The taste complaints are thought to be due to sensory deficits that arise in the absence of sufficient saliva to maintain taste receptors. We assessed the subjective complaints, salivary production and taste functioning of SS patients and unaffected individuals. We found the expected decreased salivary gland function and increased frequency of taste complaints. Our taste assessment with weak stimuli confirmed and expanded the previous report of decreased taste threshold sensitivity in SS. However, perception of stronger taste stimuli was not impaired. In contrast with previous reports, patient judgments of intensity were not significantly reduced for any of the four taste qualities. Although the salivary gland function of all patients was markedly impaired relative to that of controls, patients lacking measurable salivary flow were no more likely than patients with residual function to exhibit subjective complaints or taste impairments. Our observations are inconsistent with a simple causal chain running from salivary gland dysfunction to sensory loss to taste complaints. RP NIDR, CLIN INVEST & PATIENT CARE BRANCH, BLDG 10, ROOM 1-N-114, BETHESDA, MD 20892 USA. NR 14 TC 30 Z9 30 U1 0 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD JAN PY 1995 VL 57 IS 1 BP 89 EP 96 DI 10.1016/0031-9384(94)00211-M PG 8 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA PX426 UT WOS:A1995PX42600014 PM 7878130 ER PT S AU BEUTLER, JA CARDELLINA, JH MCMAHON, JB SHOEMAKER, RH BOYD, MR AF BEUTLER, JA CARDELLINA, JH MCMAHON, JB SHOEMAKER, RH BOYD, MR BE Arnason, JT Mata, R Romeo, JT TI Antiviral and antitumor plant metabolites SO PHYTOCHEMISTRY OF MEDICINAL PLANTS SE RECENT ADVANCES IN PHYTOCHEMISTRY LA English DT Proceedings Paper CT 34th Annual Meeting of the Phytochemical-Society-of-North-America on Phytochemistry of Medicinal Plants CY AUG 15-19, 1994 CL MEXICO CITY, MEXICO SP Phytochem Soc N Amer RP NCI,FREDERICK CANC RES & DEV CTR,DIV CANC TREATMENT,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0079-9920 BN 0-306-45181-6 J9 RECENT ADV PHYTOCHEM PY 1995 VL 29 BP 47 EP 64 PG 18 WC Plant Sciences; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA BE20P UT WOS:A1995BE20P00003 ER PT B AU Cardellina, JH Boyd, MR AF Cardellina, JH Boyd, MR BE Hostettmann, K Marston, A Maillard, M Hamburger, M TI Pursuit of new leads to antitumour and anti-HIV agents from plants SO PHYTOCHEMISTRY OF PLANTS USED IN TRADITIONAL MEDICINE SE PROCEEDINGS OF THE PHYTOCHEMICAL SOCIETY OF EUROPE LA English DT Proceedings Paper CT International Symposium of the Phytochemical-Society-of-Europe on Phytochemistry of Plants Used in Traditional Medicine CY SEP 01-OCT 01, 1993 CL LAUSANNE, SWITZERLAND SP Acad Suisse Sci Naturelles, Arkopharma, Bioforce AG, Blanc Labo SA, Buchi AG, Chapman & Hall, Ciba Geigy AG, Elsevier Sci Publ BV, Galenica SA, Gustav Fischer Verlag, Hoffmann LaRoche, John Wiley & Sons Ltd, Nestle SA, Perkin Elmer SA, Pharma Tech Res Corp, Pharmacia Biotech AG, Pharmaton SA, Sandoz AG, Schaper & Brummer GmbH, Soc Banque Suisse, Spagomed AG, Spectronex AG, Swets Publishing Serv, Varian Int AG, Zeller & Sohne AG, Zyma SA C1 NCI,DIV CANC TREATMENT,LAB DRUG DISCOVERY RES & DEV,DEV THERAPEUT PROGRAM,FREDERICK,MD 21702. NR 0 TC 3 Z9 3 U1 0 U2 0 PU OXFORD UNIVERSITY PRESS PI OXFORD PA WALTON ST, OXFORD, ENGLAND OX2 6DP BN 0-19-857775-3 J9 PR PHYT SOC PY 1995 VL 37 BP 81 EP 93 PG 13 WC Plant Sciences; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Plant Sciences; Chemistry; Pharmacology & Pharmacy GA BE97G UT WOS:A1995BE97G00004 ER PT S AU VISWANATHAN, M CAPSONI, S SAAVEDRA, JM AF VISWANATHAN, M CAPSONI, S SAAVEDRA, JM BE Fraschini, F Reiter, RJ Stankov, B TI Melatonin receptors in brain and peripheral arteries SO PINEAL GLAND AND ITS HORMONES: FUNDAMENTALS AND CLINICAL PERSPECTIVES SE NATO ADVANCED SCIENCE INSTITUTES SERIES, SERIES A, LIFE SCIENCES LA English DT Proceedings Paper CT NATO Advanced Study Institute on the Pineal Gland and Its Hormones - Fundamentals and Clinical Perspectives CY JUN 07-13, 1994 CL ERICE, ITALY SP NATO C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0258-1213 BN 0-306-45105-0 J9 NATO ADV SCI INST SE PY 1995 VL 277 BP 75 EP 81 PG 7 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Neurosciences SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Neurosciences & Neurology GA BE20J UT WOS:A1995BE20J00007 ER PT B AU HSIEH, RKC AF HSIEH, RKC BE Chen, CC TI International programs of the National Library of Medicine SO PLANNING GLOBAL INFORMATION INFRASTRUCTURE LA English DT Proceedings Paper CT 7th International Conference on New Information Technology (NIT 94) CY 1994 CL ALEXANDRIA, VA SP US Natl Commiss Lib & Informat Sci C1 NATL LIB MED,INT PROGRAMS,BETHESDA,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ABLEX PUBL CORP PI NORWOOD PA 355 CHESTNUT STREET, NORWOOD, NJ 07648 BN 1-56750-200-8 PY 1995 BP 157 EP 159 PG 3 WC Information Science & Library Science SC Information Science & Library Science GA BD62C UT WOS:A1995BD62C00015 ER PT S AU LEONMONZON, ME ILLA, I DALAKAS, MC AF LEONMONZON, ME ILLA, I DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI EXPRESSION OF POLIOVIRUS RECEPTOR IN HUMAN SPINAL-CORD AND MUSCLE SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID TRANSGENIC MICE; VIRUS RECEPTOR; CELLS; RETROVIRUS C1 NINCDS,MED NEUROL BRANCH,BETHESDA,MD 20892. NR 24 TC 20 Z9 20 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 48 EP 57 DI 10.1111/j.1749-6632.1995.tb27530.x PG 10 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00005 PM 7611659 ER PT S AU ILLA, I LEONMONZON, M AGBOATWALLA, M DURESAMIN, A DALAKAS, MC AF ILLA, I LEONMONZON, M AGBOATWALLA, M DURESAMIN, A DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI ROLE OF THE MUSCLE IN ACUTE POLIOMYELITIS INFECTION SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID HUMAN POLIOVIRUS RECEPTOR; TRANSGENIC MICE; EXPRESSION; DISEASE C1 NINCDS,NEUROMUSCULAR DIS SECT,MED NEUROL BRANCH,BETHESDA,MD 20892. CIV HOSP,DEPT PEDIAT,KARACHI,PAKISTAN. NR 24 TC 6 Z9 6 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 58 EP 67 DI 10.1111/j.1749-6632.1995.tb27531.x PG 10 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00006 PM 7611660 ER PT S AU DALAKAS, MC AF DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI THE POSTPOLIO SYNDROME AS AN EVOLVED CLINICAL ENTITY - DEFINITION AND CLINICAL DESCRIPTION SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID POSTPOLIOMYELITIS MUSCULAR-ATROPHY; AMYOTROPHIC LATERAL SCLEROSIS; NEUROMUSCULAR SYMPTOMS; PARALYTIC POLIOMYELITIS; DISEASE RP DALAKAS, MC (reprint author), NINCDS,MED NEUROL BRANCH,BLDG 10,ROOM 4N248,10 CTR DR,MSC 1382,BETHESDA,MD 20892, USA. NR 63 TC 92 Z9 95 U1 0 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 68 EP 80 DI 10.1111/j.1749-6632.1995.tb27532.x PG 13 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00007 PM 7611661 ER PT S AU SONIES, BC DALAKAS, MC AF SONIES, BC DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI PROGRESSION OF ORAL-MOTOR AND SWALLOWING SYMPTOMS IN THE POSTPOLIO SYNDROME SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID DYSPHAGIA C1 NINCDS,NEUROMUSC DIS SECT,MED NEUROL BRANCH,BETHESDA,MD 20892. NIH,CTR CLIN,DEPT REHABIL MED,BETHESDA,MD 20892. NR 15 TC 6 Z9 6 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 87 EP 95 PG 9 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00009 ER PT S AU GRAFMAN, J CLARK, K RICHARDSON, D DINSMORE, S STEIN, D DALAKAS, MC AF GRAFMAN, J CLARK, K RICHARDSON, D DINSMORE, S STEIN, D DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI NEUROPSYCHOLOGY OF POSTPOLIO SYNDROME SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID POSTPOLIO SEQUELAE; SURVIVORS; BEHAVIOR; FATIGUE C1 NINCDS,MED NEUROL BRANCH,NEUROMUSC DIS SECT,BETHESDA,MD 20892. RP GRAFMAN, J (reprint author), NINCDS,MED NEUROL BRANCH,COGNIT NEUROSCI SECT,BLDG 10,ROOM 5S209,BETHESDA,MD 20892, USA. OI Grafman, Jordan H./0000-0001-8645-4457 NR 22 TC 12 Z9 12 U1 0 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 103 EP 110 DI 10.1111/j.1749-6632.1995.tb27536.x PG 8 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00011 PM 7611618 ER PT S AU DALAKAS, MC AF DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI PATHOGENETIC MECHANISMS OF POSTPOLIO SYNDROME - MORPHOLOGICAL, ELECTROPHYSIOLOGICAL, VIROLOGICAL, AND IMMUNOLOGICAL CORRELATIONS SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID POSTPOLIOMYELITIS MUSCULAR-ATROPHY; CELL-ADHESION MOLECULE; NEUROMUSCULAR SYMPTOMS; MOTOR UNIT; PARALYTIC POLIOMYELITIS; OLD POLIOMYELITIS; HUMAN-MUSCLE; N-CAM; TRANSMISSION; ANTIBODIES RP DALAKAS, MC (reprint author), NINCDS,MED NEUROL BRANCH,BLDG 10,ROOM 4N248,10 CTR DR,MSC 1382,BETHESDA,MD 20892, USA. NR 65 TC 70 Z9 73 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 167 EP 185 DI 10.1111/j.1749-6632.1995.tb27543.x PG 19 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00018 PM 7611626 ER PT S AU ISAACSON, SH SIVAKUMAR, K ASHER, DM POMEROY, KL RAMOSALVAREZ, M GIBBS, CJ GAJDUSEK, DC DALAKAS, MC AF ISAACSON, SH SIVAKUMAR, K ASHER, DM POMEROY, KL RAMOSALVAREZ, M GIBBS, CJ GAJDUSEK, DC DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI CELLULAR-LOCALIZATION OF POLIOVIRUS RNA IN THE SPINAL-CORD DURING ACUTE PARALYTIC POLIOMYELITIS SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID POLYMERASE CHAIN-REACTION; MICE; INFECTION; AMPLIFICATION; PATHOGENESIS; DNA; PERSISTENCE C1 NINCDS,MED NEUROL BRANCH,DIV NEUROMUSC,BETHESDA,MD 20892. RP ISAACSON, SH (reprint author), NINCDS,LCNSS,BLDG 36,ROOM 5B-21,BETHESDA,MD 20892, USA. NR 31 TC 11 Z9 11 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 194 EP 200 DI 10.1111/j.1749-6632.1995.tb27545.x PG 7 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00020 PM 7611628 ER PT S AU LEONMONZON, ME DALAKAS, MC AF LEONMONZON, ME DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI DETECTION OF POLIOVIRUS ANTIBODIES AND POLIOVIRUS GENOME IN PATIENTS WITH THE POSTPOLIO SYNDROME SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID DEFECTIVE INTERFERING PARTICLES; NEUROMUSCULAR SYMPTOMS; POLIOMYELITIS; INFECTION; CELLS; ENRICHMENT; MECHANISMS C1 NINCDS, MED NEUROL BRANCH, BETHESDA, MD 20892 USA. NR 25 TC 28 Z9 28 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-918-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 208 EP 218 DI 10.1111/j.1749-6632.1995.tb27547.x PG 11 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00022 PM 7611630 ER PT S AU STEIN, DP DAMBROSIA, JM DALAKAS, MC AF STEIN, DP DAMBROSIA, JM DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL OF AMANTADINE FOR THE TREATMENT OF FATIGUE IN PATIENTS WITH THE POSTPOLIO SYNDROME SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID MULTIPLE-SCLEROSIS; SCALE; RELIABILITY; CONDUCTION; SYMPTOMS; VALIDITY C1 NINCDS, MED NEUROL BRANCH, NEUROMUSCULAR DIS SECT, BETHESDA, MD 20892 USA. NR 23 TC 32 Z9 33 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-918-5 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 296 EP 302 DI 10.1111/j.1749-6632.1995.tb27555.x PG 7 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00030 PM 7611638 ER PT S AU DINSMORE, S DAMBROSIA, J DALAKAS, MC AF DINSMORE, S DAMBROSIA, J DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI A DOUBLE-BLIND, PLACEBO-CONTROLLED TRIAL OF HIGH-DOSE PREDNISONE FOR THE TREATMENT OF POSTPOLIOMYELITIS SYNDROME SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID NEUROMUSCULAR SYMPTOMS; OLD POLIOMYELITIS; POSTPOLIOMYELITIS; SCLEROSIS; SUBSET C1 NINCDS,MED NEUROL BRANCH,NEUROMUSCULAR DIS SECT,BETHESDA,MD 20892. NR 29 TC 27 Z9 28 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 303 EP 313 DI 10.1111/j.1749-6632.1995.tb27556.x PG 11 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00031 PM 7611639 ER PT S AU DALAKAS, MC AF DALAKAS, MC BE Dalakas, MC Bartfeld, H Kurland, LT TI HOW TO DESIGN A THERAPEUTIC STUDY IN PATIENTS WITH THE POSTPOLIO SYNDROME - METHODOLOGICAL CONCERNS AND STATUS OF PRESENT THERAPIES SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI ID NEUROMUSCULAR SYMPTOMS AB Designing a therapeutic study in patients with the post-polio syndrome (PPS) is not simple because of the many variables that play a role in patient selection and definition of therapeutic outcome. Because of the asymmetry of the initial involvement, post-polio patients differ from each other in the degree of the initial disease, subsequent residual disability, and severity of new muscle symptoms. In addition, they demonstrate differences among their own limbs in reference to the severity of the initially involved limbs, the degree of the subsequent recovery, and the degree the new post-polio symptoms affect different muscles in each limb. The asymmetry and the different degree of compensation between strong and weak limbs or muscles of the same limb are additional variables that make the comparison between patient groups difficult. In spite of the inherent variables, a controlled study can be performed if the investigators are careful in patient selection and definition of the improvement from the beginning of the study. This paper focuses on the most critical variables we have faced in the various therapeutic studies we have conducted or designed in the treatment of patients with the post-polio syndrome. RP DALAKAS, MC (reprint author), NINCDS,MED NEUROL BRANCH,BLDG 10,ROOM 4N248,10 CTR DR MSC 1382,BETHESDA,MD 20892, USA. NR 18 TC 4 Z9 4 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 314 EP 320 PG 7 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00032 ER PT S AU DALAKAS, MC BARTFELD, H KURLAND, LT AF DALAKAS, MC BARTFELD, H KURLAND, LT BE Dalakas, MC Bartfeld, H Kurland, LT TI CONCLUDING REMARKS AND FUTURE STRATEGIES SO POST-POLIO SYNDROME: ADVANCES IN THE PATHOGENESIS AND TREATMENT SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on the Post-Polio Syndrome - Advances in the Pathogenesis and Treatment CY APR 27-30, 1994 CL BETHESDA, MD SP NEW YORK ACAD SCI C1 ST VINCENTS HOSP & MED CTR,CLIN RES CTR,ALS,NEW YORK,NY 10011. NYU,MED CTR,POST POLIO SYNDROME RES CTR,NEW YORK,NY 10016. MAYO CLIN & MAYO FDN,CLIN EPIDEMIOL SECT,ROCHESTER,MN 55905. RP DALAKAS, MC (reprint author), NINCDS,MED NEUROL BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-917-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 753 BP 366 EP 369 DI 10.1111/j.1749-6632.1995.tb27563.x PG 4 WC Infectious Diseases; Multidisciplinary Sciences; Pathology SC Infectious Diseases; Science & Technology - Other Topics; Pathology GA BD17U UT WOS:A1995BD17U00038 ER PT S AU FOUTS, JR PIVER, WT AF FOUTS, JR PIVER, WT BE Carmichael, GR Folk, GE Schnoor, JL TI Impacts of climate change on human health SO PREPARING FOR GLOBAL CHANGE: A MIDWESTERN PERSPECTIVE SE PROGRESS IN BIOMETEOROLOGY LA English DT Proceedings Paper CT 2nd Symposium on Global Change - A Midwest Perspective CY APR 07-08, 1994 CL UNIV IOWA, IOWA CITY, IA SP Ctr Global & Reg Environm Res, UN Assoc, Iowa Div, Int Soc Biometeorol HO UNIV IOWA C1 NIEHS,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU S P B ACADEMIC PUBL BV PI AMSTERDAM PA PO BOX 11188, 1001 GD AMSTERDAM, NETHERLANDS SN 0923-0688 BN 90-5103-102-5 J9 PROG BIOMET PY 1995 VL 9 BP 225 EP 246 PG 22 WC Ecology; Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA BE06P UT WOS:A1995BE06P00019 ER PT B AU Lands, WEM AF Lands, WEM GP AMER HEART ASSOC, COUNCIL ARTERIOSCLEROSIS TI State of the art: Where are we and where are we going? SO PROCEEDINGS FROM THE SCIENTIFIC CONFERENCE ON OMEGA-3 FATTY ACIDS IN NUTRITION, VASCULAR BIOLOGY, AND MEDICINE LA English DT Proceedings Paper CT Scientific Conference on Omega-3 Fatty Acids in Nutrition, Vascular Biology, and Medicine CY APR 17-19, 1994 CL HOUSTON, TX SP Amer Heart Assoc, Natl Heart Lung & Blood Inst RP Lands, WEM (reprint author), NIAAA,DIV BASIC RES,NIH,6000 EXECUT BLVD,ROCKVILLE,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMERICAN HEART ASSOCIATION PI DALLAS PA 7171 GREENVILLE AVE, DALLAS, TX 75231-4596 BN 0-87493-007-3 PY 1995 BP 1 EP 8 PG 8 WC Cardiac & Cardiovascular Systems; Nutrition & Dietetics; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Nutrition & Dietetics GA BG93D UT WOS:A1995BG93D00001 ER PT B AU Salem, N AF Salem, N GP AMER HEART ASSOC, COUNCIL ARTERIOSCLEROSIS TI Biophysical properties and peroxidation - Introduction SO PROCEEDINGS FROM THE SCIENTIFIC CONFERENCE ON OMEGA-3 FATTY ACIDS IN NUTRITION, VASCULAR BIOLOGY, AND MEDICINE LA English DT Proceedings Paper CT Scientific Conference on Omega-3 Fatty Acids in Nutrition, Vascular Biology, and Medicine CY APR 17-19, 1994 CL HOUSTON, TX SP Amer Heart Assoc, Natl Heart Lung & Blood Inst RP Salem, N (reprint author), NIAAA,LAB MEMBRANE BIOCHEM & BIOPHYS,NIH,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMERICAN HEART ASSOCIATION PI DALLAS PA 7171 GREENVILLE AVE, DALLAS, TX 75231-4596 BN 0-87493-007-3 PY 1995 BP 110 EP 111 PG 2 WC Cardiac & Cardiovascular Systems; Nutrition & Dietetics; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Nutrition & Dietetics GA BG93D UT WOS:A1995BG93D00011 ER PT B AU Salem, N Pawlosky, RJ AF Salem, N Pawlosky, RJ GP AMER HEART ASSOC, COUNCIL ARTERIOSCLEROSIS TI In vivo studies of the supply of polyunsaturates to the brain in various mammals SO PROCEEDINGS FROM THE SCIENTIFIC CONFERENCE ON OMEGA-3 FATTY ACIDS IN NUTRITION, VASCULAR BIOLOGY, AND MEDICINE LA English DT Proceedings Paper CT Scientific Conference on Omega-3 Fatty Acids in Nutrition, Vascular Biology, and Medicine CY APR 17-19, 1994 CL HOUSTON, TX SP Amer Heart Assoc, Natl Heart Lung & Blood Inst RP Salem, N (reprint author), NIAAA,LAB MEMBRANE BIOCHEM & BIOPHYS,NIH,12501 WASHINGTON AVE,ROCKVILLE,MD 20852, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMERICAN HEART ASSOCIATION PI DALLAS PA 7171 GREENVILLE AVE, DALLAS, TX 75231-4596 BN 0-87493-007-3 PY 1995 BP 200 EP 204 PG 5 WC Cardiac & Cardiovascular Systems; Nutrition & Dietetics; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Nutrition & Dietetics GA BG93D UT WOS:A1995BG93D00025 ER PT S AU Nelson, RG McCance, DR Pettitt, DJ AF Nelson, RG McCance, DR Pettitt, DJ BE Savage, GP TI Foetal nutrition and the risk of non-insulin-dependent diabetes mellitus in Pima Indians SO PROCEEDINGS OF THE NUTRITION SOCIETY OF NEW ZEALAND, VOL 20 SE PROCEEDINGS OF THE NUTRITION SOCIETY OF NEW ZEALAND LA English DT Proceedings Paper CT 1995 Annual Conference of the Nutrition-Society-of-New-Zealand/Australasian-Clinical-Nutrition-Society CY AUG, 1995 CL AUCKLAND MED SCH, AUCKLAND, NEW ZEALAND SP Nutr Soc New Zealand, Australasian Clin Nutr Soc HO AUCKLAND MED SCH C1 NIDDK,PHOENIX EPIDEMIOL & CLIN RES BRANCH,NATL INST HLTH,PHOENIX,AZ. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NUTRITION SOC NEW ZEALAND PI CANTERBURY PA ANIMAL & VETERINARY SCI GROUP, LINCOLN UNIVERSITY, PO BOX 84, CANTERBURY, NEW ZEALAND SN 0110-4187 J9 P NUT SOC N PY 1995 VL 20 BP 89 EP 95 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA BF95V UT WOS:A1995BF95V00010 ER PT B AU TASAKI, I AF TASAKI, I BE Ogli, K TI Effects of anesthetics on nerve fibers and cells: A macromolecular interpretation SO PROGRESS IN ANESTHETIC MECHANISM, VOL 3, SPECIAL ISSUE, 1995: PROCEEDINGS OF THE INTERNATIONAL WORKSHOP ON ANESTHETIC MECHANISMS LA English DT Proceedings Paper CT International Workshop on Anesthetic Mechanisms CY DEC 12-14, 1994 CL TAKAMATSU, JAPAN SP Kagawa Prefecture, Takamatsu City, Nankai Scholarship Fdn C1 NIMH,CELL BIOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RESEARCH GROUP ANESTHETIC MECHANISM JAPAN PI KAGAWA PA IKENOBE, MIKI, KITA, KAGAWA 760-07, JAPAN PY 1995 BP 194 EP 199 PG 6 WC Anesthesiology; Biochemistry & Molecular Biology; Pharmacology & Pharmacy SC Anesthesiology; Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA BE26E UT WOS:A1995BE26E00024 ER PT B AU MOODY, EJ HARRIS, BD SKOLNICK, P AF MOODY, EJ HARRIS, BD SKOLNICK, P BE Ogli, K TI Isoflurane stereoselectively modulates the GABA(A) receptor SO PROGRESS IN ANESTHETIC MECHANISM, VOL 3, SPECIAL ISSUE, 1995: PROCEEDINGS OF THE INTERNATIONAL WORKSHOP ON ANESTHETIC MECHANISMS LA English DT Proceedings Paper CT International Workshop on Anesthetic Mechanisms CY DEC 12-14, 1994 CL TAKAMATSU, JAPAN SP Kagawa Prefecture, Takamatsu City, Nankai Scholarship Fdn C1 NIDDK,NEUROSCI LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU RESEARCH GROUP ANESTHETIC MECHANISM JAPAN PI KAGAWA PA IKENOBE, MIKI, KITA, KAGAWA 760-07, JAPAN PY 1995 BP 433 EP 438 PG 6 WC Anesthesiology; Biochemistry & Molecular Biology; Pharmacology & Pharmacy SC Anesthesiology; Biochemistry & Molecular Biology; Pharmacology & Pharmacy GA BE26E UT WOS:A1995BE26E00069 ER PT S AU PEGG, AE DOLAN, ME MOSCHEL, RC AF PEGG, AE DOLAN, ME MOSCHEL, RC BE Cohn, WE Moldave, K TI STRUCTURE, FUNCTION, AND INHIBITION OF O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE SO PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 51 SE PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY LA English DT Review ID HUMAN O6-METHYLGUANINE-DNA METHYLTRANSFERASE; HUMAN O6-ALKYLGUANINE-DNA ALKYLTRANSFERASE; METHYL-N-NITROSOUREA; HUMAN-TUMOR-CELLS; HUMAN O-6-METHYLGUANINE-DNA METHYLTRANSFERASE; SISTER-CHROMATID EXCHANGES; NUCLEOTIDE EXCISION REPAIR; HAMSTER OVARY CELLS; RAT HEPATOMA-CELLS; HUMAN 06-ALKYLGUANINE-DNA ALKYLTRANSFERASE C1 PENN STATE UNIV, COLL MED, DEPT PHARMACOL, HERSHEY, PA 17033 USA. UNIV CHICAGO, HEMATOL ONCOL SECT, CHICAGO, IL 60637 USA. NCI, FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, FREDERICK, MD 21702 USA. RP PEGG, AE (reprint author), PENN STATE UNIV, COLL MED, DEPT CELLULAR & MOLEC PHYSIOL, HERSHEY, PA 17033 USA. FU NCI NIH HHS [CA-47228, CA-18137, CA-57725] NR 280 TC 431 Z9 440 U1 2 U2 12 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0079-6603 BN 0-12-540051-9 J9 PROG NUCLEIC ACID RE JI Prog. Nucl. Res. Molec. Biol. PY 1995 VL 51 BP 167 EP 223 DI 10.1016/S0079-6603(08)60879-X PG 57 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BE17N UT WOS:A1995BE17N00005 PM 7659775 ER PT J AU OWENS, IS RITTER, JK AF OWENS, IS RITTER, JK TI GENE STRUCTURE AT THE HUMAN UGT1 LOCUS CREATES DIVERSITY IN ISOZYME STRUCTURE, SUBSTRATE-SPECIFICITY, AND REGULATION SO PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 51 SE PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY LA English DT Review ID BILIRUBIN-UDP-GLUCURONOSYLTRANSFERASE; POLYCYCLIC AROMATIC-COMPOUNDS; CRIGLER-NAJJAR SYNDROME; HUMAN-LIVER; DEVELOPMENTAL EXPRESSION; HYDROXYLASE INDUCTION; PHENOLIC ANTIOXIDANTS; COMPLEX LOCUS; COS-1 CELLS; I PATIENT C1 VIRGINIA COMMONWEALTH UNIV,DEPT PHARMACOL & TOXICOL,RICHMOND,VA 23298. RP OWENS, IS (reprint author), NICHHD,HUMAN GENET BRANCH,GENET DISORDERS DRUG METAB SECT,BETHESDA,MD 20892, USA. NR 80 TC 57 Z9 57 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0278-5846 J9 PROG NUCLEIC ACID RE PY 1995 VL 51 BP 305 EP 338 DI 10.1016/S0079-6603(08)60882-X PG 34 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BE17N UT WOS:A1995BE17N00008 PM 7659777 ER PT J AU Osborne, NN Chader, GJ AF Osborne, NN Chader, GJ TI Untitled SO PROGRESS IN RETINAL AND EYE RESEARCH LA English DT Editorial Material C1 NEI,VIS RES LAB,BETHESDA,MD 20892. RP Osborne, NN (reprint author), UNIV OXFORD,NUFFIELD LAB OPHTHALMOL,WALTON ST,OXFORD OX2 6AW,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 1350-9462 J9 PROG RETIN EYE RES JI Prog. Retin. Eye Res. PY 1995 VL 15 IS 1 BP R7 EP R7 DI 10.1016/1350-9462(95)90000-4 PG 1 WC Ophthalmology SC Ophthalmology GA TM872 UT WOS:A1995TM87200001 ER PT J AU ROBISON, WG LAVER, NM LOU, MF AF ROBISON, WG LAVER, NM LOU, MF TI THE ROLE OF ALDOSE REDUCTASE IN DIABETIC-RETINOPATHY - PREVENTION AND INTERVENTION STUDIES SO PROGRESS IN RETINAL AND EYE RESEARCH LA English DT Review ID GALACTOSE-FED DOGS; RETINAL MURAL CELLS; WEIGHT AGGREGATE FORMATION; LENS CRYSTALLIN GLYCATION; COTTON-WOOL SPOTS; PROLIFERATIVE RETINOPATHY; RADIATION RETINOPATHY; INHIBITOR TOLRESTAT; INTRAMURAL PERICYTES; CATARACT DEVELOPMENT C1 GEORGETOWN UNIV, SCH MED, DEPT PATHOL, WASHINGTON, DC 20007 USA. UNIV NEBRASKA, DEPT VET & BIOMED SCI, LINCOLN, NE 68583 USA. RP ROBISON, WG (reprint author), NEI, PATHOPHYSIOL SECT, BLDG 6, ROOM 316, BETHESDA, MD 20892 USA. NR 291 TC 24 Z9 25 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1350-9462 J9 PROG RETIN EYE RES JI Prog. Retin. Eye Res. PY 1995 VL 14 IS 2 BP 593 EP 640 DI 10.1016/1350-9462(94)00020-G PG 48 WC Ophthalmology SC Ophthalmology GA RJ449 UT WOS:A1995RJ44900012 ER PT J AU OSBORNE, N CHADER, J AF OSBORNE, N CHADER, J TI UNTITLED SO PROGRESS IN RETINAL AND EYE RESEARCH LA English DT Editorial Material C1 NEI,VIS RES LAB,BETHESDA,MD 20892. RP OSBORNE, N (reprint author), UNIV OXFORD,NUFFIELD LAB OPHTHALMOL,WALTON ST,OXFORD OX2 6AW,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 1350-9462 J9 PROG RETIN EYE RES JI Prog. Retin. Eye Res. PY 1995 VL 14 IS 2 BP R7 EP R7 PG 1 WC Ophthalmology SC Ophthalmology GA RJ449 UT WOS:A1995RJ44900001 ER PT B AU Simonsick, EM AF Simonsick, EM BE Bond, LA Cutler, SJ Grams, A TI Demography of productive aging SO PROMOTING SUCCESSFUL AND PRODUCTIVE AGING SE PRIMARY PREVENTION OF PSYCHOPATHOLOGY LA English DT Proceedings Paper CT 16th Vermont Conference on Primary Prevention of Psychopathology (VCPPP) - Promoting Successful and Productive Aging CY JUN, 1993 CL UNIV VERMONT, BURLINGTON, VT SP Univ Vermont, Ctr Study Aging, NIMH HO UNIV VERMONT C1 NIA,EPIDEMIOL DEMOG & BIOMETRY PROGRAM,BETHESDA,MD 20892. NR 0 TC 5 Z9 6 U1 0 U2 0 PU SAGE PUBLICATIONS INC PI NEWBURY PK PA 2455 TELLER RD, NEWBURY PK, CA 91320 BN 0-8039-7171-0 J9 PRIM PREV P PY 1995 VL 16 BP 69 EP 89 PG 3 WC Geriatrics & Gerontology; Psychology; Social Work SC Geriatrics & Gerontology; Psychology; Social Work GA BG50L UT WOS:A1995BG50L00004 ER PT S AU ELING, TE EVERHART, AL GLASGOW, WC AF ELING, TE EVERHART, AL GLASGOW, WC BE Samuelsson, B Ramwell, PW Paoletti, R Folco, G Granstrom, E Nicosia, S TI MODULATION OF EGF CELL SIGNALING TYROSINE PHOSPHORYLATION BY LINOLEIC-ACID METABOLITES SO PROSTAGLANDINS AND RELATED COMPOUNDS: NINTH INTERNATIONAL CONFERENCE, FLORENCE, ITALY SE ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH LA English DT Article; Proceedings Paper CT 9th International Conference on Prostaglandins and Related Compounds CY JUN 06-10, 1994 CL FLORENCE, ITALY ID EPIDERMAL GROWTH-FACTOR RP ELING, TE (reprint author), NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 5 TC 2 Z9 2 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0361-5952 BN 0-7817-0238-0 J9 ADV PROSTAG THROMB L PY 1995 VL 23 BP 453 EP 455 PG 3 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism GA BC44Z UT WOS:A1995BC44Z00099 PM 7537437 ER PT B AU RAY, JM STETLERSTEVENSON, WG AF RAY, JM STETLERSTEVENSON, WG BE Suzuki, M Hiwasa, T TI Gelatinase A and TIMP-2 alter human melanoma cell adhesion SO PROTEASES INVOLVED IN CANCER LA English DT Proceedings Paper CT Chiba International Symposium on Cancer: Proteases Involved in Cancer CY NOV 07, 1994 CL CHIBA, JAPAN RP NIH,PATHOL LAB,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MONDUZZI EDITORE PI 40128 BOLOGNA PA VIA FERRARESE 119/2, 40128 BOLOGNA, ITALY BN 88-323-1107-0 PY 1995 BP 89 EP 95 PG 7 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA BD24U UT WOS:A1995BD24U00012 ER PT J AU GEORGIOU, PE CHEN, SL SUZUKI, H ROMANOSPICA, V LAUTENBERGER, JA PAPAS, TS BHAT, NK AF GEORGIOU, PE CHEN, SL SUZUKI, H ROMANOSPICA, V LAUTENBERGER, JA PAPAS, TS BHAT, NK TI EXPRESSION OF ETS FAMILY GENES IN SYSTEMIC LUPUS-ERYTHEMATOSUS SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 HOKKAIDO UNIV,SAPPORO,HOKKAIDO 060,JAPAN. MED UNIV S CAROLINA,CHARLESTON,SC 29425. FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21701. RP GEORGIOU, PE (reprint author), NCI,FREDERICK CANC RES & DEV CTR,MOLEC ONCOL LAB,FREDERICK,MD 21701, USA. NR 3 TC 0 Z9 0 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 34 EP 34 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200054 ER PT J AU BALER, R GUETTOUCHE, T VOELLMY, R AF BALER, R GUETTOUCHE, T VOELLMY, R TI ON THE MODEL OF FEEDBACK-REGULATION OF HEAT-SHOCK GENE-EXPRESSION BY HEAT-SHOCK PROTEINS - DEMONSTRATION OF HEAT-SHOCK PROTEIN-70-CONTAINING COMPLEXES OF UNACTIVATED HEAT-SHOCK TRANSCRIPTION FACTOR-1 SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 NIH,BETHESDA,MD 20892. RP BALER, R (reprint author), UNIV MIAMI,SCH MED,DEPT BIOCHEM & MOLEC BIOL,MIAMI,FL 33136, USA. NR 4 TC 2 Z9 2 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 35 EP 35 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200055 ER PT J AU ROBINSON, L THOMPSON, D SETH, A AF ROBINSON, L THOMPSON, D SETH, A TI ETS TARGET GENES - INTERACTION OF ETS PROTEINS WITH THE SERUM RESPONSE FACTOR ON THE EARLY GROWTH-RESPONSE GENE-1 PROMOTER SERUM RESPONSE ELEMENT SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co ID FAMILY C1 FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21701. RP ROBINSON, L (reprint author), NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 76 EP 76 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200116 ER PT J AU VENANZONI, M ROBINSON, L THOMPSON, D LI, H SETH, A AF VENANZONI, M ROBINSON, L THOMPSON, D LI, H SETH, A TI REGULATION OF P53 PROMOTER BY ETS1 AND ETS2 SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21701. RP VENANZONI, M (reprint author), NCI,MOLEC ONCOL LAB,FREDERICK,MD 21701, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 78 EP 78 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200120 ER PT J AU VERRIER, CS BAILEY, LR YEE, CJ ROODI, N JENSEN, RA BUSTIN, M PARL, FF AF VERRIER, CS BAILEY, LR YEE, CJ ROODI, N JENSEN, RA BUSTIN, M PARL, FF TI THE INTERACTION OF THE ESTROGEN-RECEPTOR WITH ITS DNA RESPONSE ELEMENT IS FACILITATED BY THE HIGH-MOBILITY GROUP PROTEIN, HMG-1 SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 NCI,MOLEC CARCINOGENESIS LAB,BETHESDA,MD 20892. RP VERRIER, CS (reprint author), VANDERBILT UNIV,DEPT PATHOL,NASHVILLE,TN 37232, USA. RI Parl, Fritz/A-5116-2010; Jensen, Roy/B-9739-2011; Bustin, Michael/G-6155-2015 OI Jensen, Roy/0000-0003-4430-2281; NR 2 TC 3 Z9 3 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 78 EP 78 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200121 ER PT J AU SUZUKI, H ROMANOSPICA, V GEORGIOU, P CHEN, SL LAUTENBERGER, JA BHAT, NK AF SUZUKI, H ROMANOSPICA, V GEORGIOU, P CHEN, SL LAUTENBERGER, JA BHAT, NK TI EFFECT OF ANTISENSE ETS2 MESSENGER-RNA ON ETS2 GENE-EXPRESSION IN DLD-1 COLON-CANCER CELLS SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 FREDERICK CANC RES & DEV CTR,DYNCORP,PROGRAM RESOURCES INC,FREDERICK,MD 21702. HOKKAIDO UNIV,SAPPORO,HOKKAIDO 060,JAPAN. RP SUZUKI, H (reprint author), NCI,MOLEC ONCOL LAB,POB B,FREDERICK,MD 21702, USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 86 EP 86 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200128 ER PT J AU ZDANOVSKY, AG ZDANOVSKAIA, MV STRICKLAND, D FITZGERALD, DJ AF ZDANOVSKY, AG ZDANOVSKAIA, MV STRICKLAND, D FITZGERALD, DJ TI TARGETING OF PSEUDOMONAS AND DIPHTHERIA TOXINS TO THE ALPHA(2)-MACROGLOBULIN RECEPTOR VIA RAP-TOXIN AND PAI-1-TOXIN FUSIONS SO PROTEIN ENGINEERING LA English DT Article; Proceedings Paper CT 1995 Miami Bio/Technology Winter Symposium - Advances in Gene Technology: Protein Engineering and Structural Biology CY FEB 04-09, 1995 CL MIAMI, FL SP Univ Miami, Nature Publishing Co C1 AMER RED CROSS,BIOCHEM LAB,ROCKVILLE,MD 20855. RP ZDANOVSKY, AG (reprint author), NCI,DIV CANC BIOL DIAG & CTR,MOLEC BIOL LAB,BETHESDA,MD 20892, USA. NR 1 TC 0 Z9 0 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0269-2139 J9 PROTEIN ENG JI Protein Eng. PY 1995 VL 8 SU S BP 123 EP 123 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RL742 UT WOS:A1995RL74200178 ER PT J AU SELLERS, JR GOODSON, HV AF SELLERS, JR GOODSON, HV TI MOTOR PROTEINS .2. MYOSIN SO PROTEIN PROFILE LA English DT Review ID SMOOTH-MUSCLE MYOSIN; HEAVY-CHAIN GENE; REGULATORY LIGHT-CHAIN; AMINO-ACID-SEQUENCE; RABBIT SKELETAL-MUSCLE; ACTIN-ACTIVATED ATPASE; BRUSH-BORDER MYOSIN; SPIN-LABELED MYOSIN; FAMILIAL HYPERTROPHIC CARDIOMYOPATHY; CHICKEN GIZZARD MYOSIN C1 STANFORD UNIV, SCH MED, DEPT BIOCHEM, STANFORD, CA 94305 USA. RP NHLBI, MOLEC CARDIOL LAB, BLDG 10, BETHESDA, MD 20892 USA. FU NIGMS NIH HHS [GM 40509] NR 2373 TC 100 Z9 104 U1 1 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1070-3667 J9 PROTEIN PROFILE JI Protein Profile PY 1995 VL 2 IS 12 BP 1323 EP 1423 PG 101 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TJ716 UT WOS:A1995TJ71600001 PM 8665326 ER PT J AU LOH, YP CAWLEY, NX AF LOH, YP CAWLEY, NX TI PROCESSING ENZYMES OF PEPSIN FAMILY - YEAST ASPARTIC PROTEASE-3 AND PROOPIOMELANOCORTIN CONVERTING-ENZYME SO PROTEOLYTIC ENZYMES: ASPARTIC AND METALLO PEPTIDASES SE METHODS IN ENZYMOLOGY LA English DT Review ID PITUITARY INTERMEDIATE LOBE; SECRETORY VESICLES; PURIFICATION; PROOPIOMELANOCORTIN; SITES; GENE C1 UNIFORMED SERV UNIV HLTH SCI,DEPT BIOCHEM,BETHESDA,MD 20892. RP LOH, YP (reprint author), NICHHD,DEV NEUROBIOL LAB,CELLULAR NEUROBIOL SECT,BETHESDA,MD 20892, USA. NR 21 TC 8 Z9 8 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 248 BP 136 EP 146 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BD46R UT WOS:A1995BD46R00009 PM 7674917 ER PT J AU ASTHANA, S RAFFAELE, KC GREIG, NH BERARDI, A MORRIS, PP SCHAPIRO, MB RAPOPORT, SI BLACKMAN, MR SONCRANT, TT AF ASTHANA, S RAFFAELE, KC GREIG, NH BERARDI, A MORRIS, PP SCHAPIRO, MB RAPOPORT, SI BLACKMAN, MR SONCRANT, TT TI NEUROENDOCRINE RESPONSES TO INTRAVENOUS-INFUSION OF ARECOLINE IN PATIENTS WITH ALZHEIMERS-DISEASE SO PSYCHONEUROENDOCRINOLOGY LA English DT Article DE ALZHEIMERS DISEASE; ARECOLINE; HYPOTHALAMIC-PITUITARY-ADRENAL AXIS; MEMORY; STRESS; CORTICOSTEROID ID CORTICOTROPIN-RELEASING HORMONE; RAT HYPOTHALAMUS INVITRO; PUTATIVE NEUROTRANSMITTERS; DEXAMETHASONE SUPPRESSION; BRAIN ACETYLCHOLINE; CHOLINERGIC AGONIST; CEREBROSPINAL-FLUID; ANTERIOR-PITUITARY; NEURO-ENDOCRINE; PHYSOSTIGMINE AB We have reported that arecoline, a muscarinic receptor agonist replicably enhanced verbal memory in five of nine subjects with Alzheimer's disease (AD). To investigate the mechanism of cognitive improvement, circulating hormone measurements were made during high-dose acute and low-dose chronic intravenous (i.v.) arecoline administration to AD patients. Acute hormone responses were measured during, and for 6 h after, infusion of arecoline 5 mg i.v. over 30 min. Chronic responses were measured in cognitive responders during continuous i.v. infusion of arecoline escalating over 2 weeks (0.5-40 mg/day) and then during a 1 week infusion of the dose optimizing cognition (4-16 mg/day). Acute arecoline administered to 14 subjects produced unpleasant side-effects (e.g. nausea, vomiting), mean adrenocorticotrophic hormone (p=.0006), cortisol (p=.0001) and beta-endorphin (p=.0001) levels were elevated. During chronic arecoline treatment, no side-effects occurred and plasma cortisol, adrenocorticotrophic hormone and beta-endorphin levels were unchanged in nine subjects overall and in five cognitive responders. Thus, high-dose arecoline activates the hypothalamic-pituitary-adrenal (HPA) axis and may increase other anterior pituitary hormone levels, likely representing a 'stress response', but cognition-enhancing, low doses of arecoline do not produce a glucocorticoid response. Hence, arecoline-induced memory improvement is not due to the induction of 'stress' nor to the elevation of peripheral corticosteroid levels. C1 NIA,NEUROSCI LAB,PHARMACOL & PHARMACOKINET UNIT,BETHESDA,MD 20892. US FDA,WASHINGTON,DC 20204. HARVARD UNIV,DEPT PSYCHOL,CAMBRIDGE,MA 02138. JOHNS HOPKINS UNIV,FRANCIS SCOTT KEY MED CTR,SCH MED,DIV ENDOCRINOL,BALTIMORE,MD 21224. NR 63 TC 16 Z9 16 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4530 J9 PSYCHONEUROENDOCRINO JI Psychoneuroendocrinology PY 1995 VL 20 IS 6 BP 623 EP 636 DI 10.1016/0306-4530(94)00084-N PG 14 WC Endocrinology & Metabolism; Neurosciences; Psychiatry SC Endocrinology & Metabolism; Neurosciences & Neurology; Psychiatry GA TB087 UT WOS:A1995TB08700005 PM 8584603 ER PT J AU HENNINGFIELD, JE HEISHMAN, SJ AF HENNINGFIELD, JE HEISHMAN, SJ TI THE ADDICTIVE ROLE OF NICOTINE IN TOBACCO USE SO PSYCHOPHARMACOLOGY LA English DT Editorial Material ID ABUSE LIABILITY; SMOKING; SMOKERS; SPRAY RP HENNINGFIELD, JE (reprint author), NIDA,ADDICT RES CTR,CLIN PHARMACOL BRANCH,POB 5180,BALTIMORE,MD 21224, USA. NR 27 TC 21 Z9 21 U1 1 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 1 BP 11 EP 13 DI 10.1007/BF02245089 PG 3 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QC137 UT WOS:A1995QC13700003 PM 7724694 ER PT J AU ELMER, GI PIEPER, JO GOLDBERG, SR GEORGE, FR AF ELMER, GI PIEPER, JO GOLDBERG, SR GEORGE, FR TI OPIOID OPERANT SELF-ADMINISTRATION, ANALGESIA, STIMULATION AND RESPIRATORY DEPRESSION IN MU-DEFICIENT MICE SO PSYCHOPHARMACOLOGY LA English DT Article DE OPIOID; GENETICS; SELF-ADMINISTRATION; CXBK/BYJ; REINFORCEMENT ID OPIATE RECEPTOR CONCENTRATION; STRAIN DIFFERENCES; GENETIC-DIFFERENCES; POSITIVE REINFORCER; MOUSE STRAINS; LONG-SLEEP; MORPHINE; ETHANOL; ETONITAZENE; C57BL/6J AB It is commonly thought that mu-receptors play an important role in the reinforcing effects of opioids. In the present study, inbred strains widely divergent in CNS opiate receptor densities were used to investigate the influence of genetic variation in receptor concentration on opioid-reinforced behavior. In particular, the CXBK/ByJ mice were used as an investigative tool because of their significantly lower number of CNS mu opioid receptors. The behavioral pharmacology of opioids in the mu-deficient CXBK/ByJ mice was compared to other commonly used inbred mouse strains, C57BL/6J and BALB/cJ, and the opiate receptor rich CXBH/ByJ mice. Operant opioid reinforced behavior, opioid-induced locomotor stimulation, analgesia and respiratory depression were investigated in all four inbred strains. To assess the acquisition and maintenance of opioid reinforced behavior, oral self-administration of the potent benzimidazole opioid, etonitazene, was determined using an operant fixed-ratio schedule of reinforcement (FR 8). Acquisition of etonitazene-reinforced behavior was established in all four strains including the mu-deficient CXBK/ByJ mice. However, there were significant genetic differences in the amount of drug intake during the maintenance of opioid-reinforced behavior and extinction behavior following vehicle substitution. For example, drug intake was significantly greater in the BK versus BH mice during the maintenance phase and an extinction burst was seen in the BH but not the BK mice following vehicle substitution. Thus, mu-receptor density may not account for individual variability in the acquisition of opioid-reinforced behavior under these conditions. Sensitivity to etonitazene-induced respiratory depression, stimulation of locomotor activity and analgesia were unrelated to drug intake during self-administration sessions across these four inbred strains. These data indicate that inherited differences in CNS mu-opiate receptor concentrations do not affect acquisition of etonitazene-reinforced behavior. C1 UNIV NEW MEXICO,DEPT PSYCHOL,ALBUQUERQUE,NM 87131. UNIV NEW MEXICO,CTR ALCOHOLISM SUBST ABUSE ADDICT,ALBUQUERQUE,NM 87131. RP ELMER, GI (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL LAB,BEHAV PHARMACOL & GENET SECT,POB 5180,BALTIMORE,MD 21224, USA. FU NIAAA NIH HHS [AA-07754, AA-09549] NR 61 TC 64 Z9 66 U1 2 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 1 BP 23 EP 31 DI 10.1007/BF02245094 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QC137 UT WOS:A1995QC13700008 PM 7724699 ER PT J AU MINER, LL MARLEY, RJ AF MINER, LL MARLEY, RJ TI CHROMOSOMAL MAPPING OF LOCI INFLUENCING SENSITIVITY TO COCAINE-INDUCED SEIZURES IN BXD RECOMBINANT INBRED STRAINS OF MICE SO PSYCHOPHARMACOLOGY LA English DT Article DE COCAINE; QUANTITATIVE TRAIT LOCI; SEIZURE; RECOMBINANT INBRED STRAINS; GENETICS ID QUANTITATIVE-TRAIT-LOCI; MORPHINE; ABUSE; MOUSE AB Among inbred mice, genetic factors mediate differences in sensitivity to the convulsant properties of cocaine; however, the gene(s) underlying cocaine's effects have not been identified. To help elucidate the gene(s) responsible for cocaine seizure susceptibility, we used recombinant inbred-quantitative trait loci (RI-QTL) analyses to identify chromosomal loci associated with cocaine-induced seizures. RI-QTL analyses seek to identify associations between a quantitative measure of a particular phenotype and one or more previously mapped marker genes across a panel of RI strains. This report describes an RI-QTL analysis of cocaine seizure susceptibility among 26 BXD RI strains. These strains showed a skewed, bimodal range of seizure susceptibility which could be the result of one or more modifying genes acting in concert with a major gene to influence cocaine sensitivity. Correlating the percent seizures displayed by each strain following 60 mg/kg cocaine with chromosomal marker data for these strains revealed a number of significant correlations clustered in two regions on chromosomes 12 and 6. This is the first identification of putative chromosomal loci associated with a cocaine-related phenotype and should facilitate identification of the gene(s) underlying cocaine toxicity and other cocaine-related phenotypes. RP MINER, LL (reprint author), NIDA,ADDICT RES CTR,MOLEC NEUROBIOL BRANCH,GENET SECT,POB 5180,BALTIMORE,MD 21224, USA. NR 19 TC 16 Z9 16 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 1 BP 62 EP 66 DI 10.1007/BF02245099 PG 5 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QC137 UT WOS:A1995QC13700013 PM 7724703 ER PT J AU EGAN, MF HYDE, TM KLEINMAN, JE WYATT, RJ AF EGAN, MF HYDE, TM KLEINMAN, JE WYATT, RJ TI NEUROLEPTIC-INDUCED VACUOUS CHEWING MOVEMENTS IN RODENTS - INCIDENCE AND EFFECTS OF LONG-TERM INCREASES IN HALOPERIDOL DOSE SO PSYCHOPHARMACOLOGY LA English DT Article DE TARDIVE DYSKINESIA; RATS; HALOPERIDOL; VACUOUS CHEWING MOVEMENTS ID GLUTAMIC-ACID DECARBOXYLASE; TARDIVE-DYSKINESIA; ORAL DYSKINESIA; ANIMAL-MODEL; RATS; CLOZAPINE; RECEPTORS; D1-DOPAMINE; WITHDRAWAL; DISORDERS AB Rats treated chronically with neuroleptics develop vacuous chewing movements (VCMs), similar in some respects to tardive dyskinesia (TD) in man. The VCM syndrome was used as a model of TD to examine the ability of increased neuroleptic doses to produce long-term suppression of dyskinetic movements. The incidence and persistence of the VCM syndrome in individual rats were also assessed to look for affected and unaffected subgroups. Rats were initially treated for 15 weeks with haloperidol decanoate. For the next 21 weeks, half the group received a 50-150% increase in dose while the other half continued to receive the same dose. Animals were also followed during a 28-week withdrawal period. Total VCM ratings showed a skewed distribution, with some rats exhibiting few movements while others developed marked and persistent movements. Increasing doses did not suppress VCMs, nor did they exacerbate movements during the withdrawal period. To the extent that the VCM syndrome models TD, the absence of long-term suppression of the VCM syndrome suggests that, at this dosage range, increasing depot neuroleptic doses may not be a useful long-term strategy for TD suppression. C1 NIMH,NEUROSCI CTR ST ELIZABETHS,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. RP EGAN, MF (reprint author), NIMH,NEUROSCI CTR ST ELIZABETHS,NEUROSCI BRANCH,2700 ML KING JR AVE SE,WASHINGTON,DC 20032, USA. NR 52 TC 18 Z9 18 U1 3 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 1 BP 74 EP 81 DI 10.1007/BF02245101 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QC137 UT WOS:A1995QC13700015 PM 7724705 ER PT J AU MAZZOLAPOMIETTO, P AULAKH, CS WOZNIAK, KM HILL, JL MURPHY, DL AF MAZZOLAPOMIETTO, P AULAKH, CS WOZNIAK, KM HILL, JL MURPHY, DL TI EVIDENCE THAT 1-(2,5-DIMETHOXY-4-ODOPHENYL)-2-AMINOPROPANE (DOI)-INDUCED HYPERTHERMIA IN RATS IS MEDIATED BY STIMULATION OF 5-HT2A RECEPTORS SO PSYCHOPHARMACOLOGY LA English DT Article DE M-CHLOROPHENYLPIPERAZINE; RITANSERIN; SPIPERONE; ATTENUATED; TOLERANCE; MDL-72222; PROPRANOLOL; KETANSERIN ID ANTIDEPRESSANT TREATMENTS; SEROTONIN RECEPTORS; M-CPP; AGONIST; BINDING; BRAIN; ANTAGONISTS; DOI; NEUROENDOCRINE; IDENTIFICATION AB The effects of various 5-HT receptor subtype-selective antagonists were studied on phenylisopropylamine hallucinogen 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI)-induced hyperthermia in Wistar rats, in an attempt to characterize the 5-HT receptor subtype mediating DOI-induced hyperthermia. Intraperitoneal administration of DOI to rats produced hyperthermia with a peak effect at 60 min. Pretreatment with propranolol (beta-adrenoceptor antagonist that also has binding affinity for 5-HT1A, 5-HT1B and 5-HT2C sites), MDL-72222 or ondansetron (5-HT3 antagonists) did not attenuate DOI-induced hyperthermia. In contrast, pretreatment with metergoline (5-HT1/5-HT2 antagonist), ketanserin, LY53857, mesulergine, mianserin and ritanserin (5-HT2C/5-HT2A antagonists), as well as spiperone (5-HT1A/5-HT2A/D-2 antagonist), significantly attenuated DOI-induced hyperthermia. Furthermore, daily administration of DOI (2.5 mg/kg per day) for 17 days did not produce either tolerance to its hyperthermic effect or modify m-CPP-induced hyperthermia in rats. These findings suggest that DOI-induced hyperthermia in rats is mediated by stimulation of 5-HT2A receptors. C1 NIMH,CLIN SCI LAB,BETHESDA,MD 20892. NR 35 TC 56 Z9 56 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 2 BP 193 EP 199 DI 10.1007/BF02245187 PG 7 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QF176 UT WOS:A1995QF17600011 PM 7753967 ER PT J AU SHOAIB, M SPANAGEL, R STOHR, T SHIPPENBERG, TS AF SHOAIB, M SPANAGEL, R STOHR, T SHIPPENBERG, TS TI STRAIN DIFFERENCES IN THE REWARDING AND DOPAMINE-RELEASING EFFECTS OF MORPHINE IN RATS SO PSYCHOPHARMACOLOGY LA English DT Article DE MICRODIALYSIS; CONDITIONED PLACE PREFERENCE; MORPHINE; LOCOMOTOR ACTIVITY; GENETIC DIFFERENCES; RAT ID FREELY MOVING RATS; INBRED MOUSE STRAINS; NUCLEUS-ACCUMBENS; PLACE PREFERENCE; INDIVIDUAL VULNERABILITY; MOTIVATIONAL PROPERTIES; EXTRACELLULAR DOPAMINE; VOLUNTARY CONSUMPTION; LOCOMOTOR STIMULATION; OPIOID SYSTEMS AB Studies examining differential sensitivity to psychoactive drugs in mice suggest that genotype may play a critical role. Furthermore, an involvement of genotype in mediating individual differences in sensitivity to the rewarding effects of several drugs of abuse has also been postulated. The aim of this study was to examine the conditioned rewarding and dopamine-releasing effects of morphine in two outbred rat strains commonly used in addiction research. Additionally, the behavioural and neuroendocrine responses of these strains to the stress of novelty were also examined. Basal locomotor activity was higher in Wistar rats than Sprague-Dawley following exposure to a novel environment. In contrast, elevations in plasma corticosteroid levels following novelty exposure did not differ between the two strains. In a counterbalanced place preference conditioning procedure, increasing doses of morphine (1.0-10.0 mg/kg SC) produced significant conditioned place preferences (CPP) in both Wistar and Sprague-Dawley strains. However, Wistar rats required a significantly larger dose of morphine (5.0 mg/kg) to produce a significant CPP than the Sprague-Dawley rats. In the latter strain, CPP occurred with doses of 3.0 mg/kg and greater. In parallel microdialysis experiments, both strains showed significant dose-related increases in dopamine release in the nucleus accumbens following acute morphine challenge (1.0-10.0 mg/kg SC). Again in Wistar rats, a larger dose of morphine was necessary to produce a significant increase in comparison to Sprague-Dawley rats. These results show that genetically distinct rat strains can show differential sensitivity to opioids, more specifically to drug-seeking responses. C1 MAX PLANCK INST PSYCHIAT,INST CLIN,DRUG ABUSE GRP,D-80804 MUNICH,GERMANY. RP SHOAIB, M (reprint author), NIDA,ADDICT RES CTR,PRECLIN PHARMACOL BRANCH,BALTIMORE,MD 21224, USA. NR 36 TC 50 Z9 50 U1 1 U2 3 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD JAN PY 1995 VL 117 IS 2 BP 240 EP 247 DI 10.1007/BF02245193 PG 8 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QF176 UT WOS:A1995QF17600017 PM 7753973 ER PT J AU RUDORFER, MV AF RUDORFER, MV TI 1994 ANNUAL NCDEU MEETING WORKSHOPS AND SYMPOSIA SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material RP RUDORFER, MV (reprint author), NIMH,ROCKVILLE,MD 20857, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 1 EP 2 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500001 ER PT J AU PRIEN, RF AF PRIEN, RF TI A BRIEF-HISTORY OF THE NEW CLINICAL DRUG-EVALUATION UNIT MEETING - HOW IT BEGAN SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material RP PRIEN, RF (reprint author), NIMH,ROCKVILLE,MD 20857, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 3 EP 5 PG 3 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500002 PM 7675986 ER PT J AU RUSH, AJ PRIEN, RF AF RUSH, AJ PRIEN, RF TI FROM SCIENTIFIC KNOWLEDGE TO THE CLINICAL-PRACTICE OF PSYCHOPHARMACOLOGY - CAN THE GAP BE BRIDGED SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE STRATEGY; TACTICS; SCIENTIFIC KNOWLEDGE; CLINICAL PRACTICE; PSYCHOPHARMACOLOGY AB This paper examines the nature and types of information and syntheses relevant to the strategic and tactical decisions required in the treatment of depression with medication, Strategic decisions (What to do?) include determining what is wrong, defining treatment objectives, selecting the first treatment, deciding the next best treatment (should the first treatment fail), and deciding when to discontinue a successful treatment, Tactical decisions (How to do?) Include determining where and by whom treatment is conducted, and the specific steps involved (e.g., dosing, frequency of visits, assessment of outcome, recognition and management of side effects, compliance, etc.) in the treatment as well as in the discontinuation of treatment (e.g., taper schedule, frequency of visits, etc.). The treatment of each patient is inherently an experimental single case study, Because individual patients differ, the most luxuriant data bases cannot fully address all strategic and tactical decisions with certainty, Thus, practitioners must apply general principles and group (nomothetic) data to individual patients. The relationship between research efforts and the information needed by clinicians is examined. Suggestions for developing and reporting more clinically relevant information from efficacy trials and other re search are made. C1 UNIV TEXAS,SW MED CTR,DALLAS,TX. NIMH,CLIN TREATMENT RES BRANCH,ROCKVILLE,MD 20857. OI Rush, Augustus/0000-0003-2004-2382 FU NIMH NIH HHS [MH-41115] NR 5 TC 43 Z9 43 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 7 EP 20 PG 14 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500003 PM 7675992 ER PT J AU VITIELLO, B JENSEN, PS AF VITIELLO, B JENSEN, PS TI DEVELOPMENTAL PERSPECTIVES IN PEDIATRIC PSYCHOPHARMACOLOGY SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE PSYCHOPHARMACOLOGY; CHILDREN; DEVELOPMENT ID PSYCHIATRIC-PATIENTS; CEREBROSPINAL-FLUID; MONOAMINE-OXIDASE; NURSING RATS; CHILDREN; ADOLESCENTS; DESIPRAMINE; IMIPRAMINE; AGE; THERAPY AB Compared with adults, children and adolescents respond to psychotropic medications in some distinctive ways that Rave implications for both efficacy and safety. Age-related differences in pharmacokinetics and pharmacodynamics account for some of these differences. But while the disposition of several psychotropics in children has been studied, the pharmacodynamic effects of the interaction between the drug and the developing brain are still largely unknown, There is now evidence that the major neurotransmitter systems undergo developmental changes that can extend into the second decade of life. Data from various animal models also show that the administration of psychotropics in the pre- and perinatal period can interfere with the development and produce permanent changes in the neurotransmitter system in question. The extrapolation of these data to humans is difficult. Given the increasing use of psychotropics in the pediatric age, the need emerges for systematic investigation on long-term effects of these drugs on the developing brain. RP VITIELLO, B (reprint author), NIMH,DIV CLIN & TREATMENT RES,CHILD & ADOLESCENT DISORDERS RES BRANCH,ROOM 18C-17,ROCKVILLE,MD 20857, USA. OI Jensen, Peter/0000-0003-2387-0650 NR 60 TC 37 Z9 37 U1 2 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 75 EP 81 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500012 PM 7675993 ER PT J AU COTT, J AF COTT, J TI MEDICINAL-PLANTS AND DIETARY-SUPPLEMENTS - SOURCES FOR INNOVATIVE TREATMENTS OR ADJUNCTS - INTRODUCTION SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material DE NATURAL PRODUCTS; HERBAL MEDICINE; ETHNOPHARMACOLOGY; GINKGO; HYPERICUM ID LOCOMOTOR STIMULATION; DRUGS; SUPPRESSION; INHIBITION; HYPERICIN; BACLOFEN; BEHAVIOR; THERAPY; MICE RP COTT, J (reprint author), NIMH,DIV CLIN & TREATMENT RES,PHARMACOL TREATMENT RES PROGRAM,ROOM 18-105,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 38 TC 23 Z9 24 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 131 EP 137 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500019 PM 7675977 ER PT J AU CHUNG, IW KIM, YS AHN, JS LEE, HS CHEN, G MANJI, HK POTTER, WZ PICKAR, D AF CHUNG, IW KIM, YS AHN, JS LEE, HS CHEN, G MANJI, HK POTTER, WZ PICKAR, D TI PHARMACOLOGICAL PROFILE OF NATURAL-PRODUCTS USED TO TREAT PSYCHOTIC ILLNESSES SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article DE NATURAL PRODUCTS; TRADITIONAL MEDICINE; PSYCHOPHARMACOLOGY; MONOAMINE RECEPTORS; RADIOLIGAND RECEPTOR BINDING STUDY ID BINDING-SITES; CLOZAPINE; RAT; SCHIZOPHRENIA; MEMBRANES; LIGAND AB Natural products have a long history of being the sources for therapeutic drugs in medicine, Investigations of natural products also have the potential for enhancing our understanding about the pathophysiology and treatment of various disorders. The screening assays described here were conducted on the phytochemical and pharmacological profiles of natural products used to treat psychotic illnesses in Korean traditional medicine, which are eligibly archived in Tongeuibogam, a traditional medicinal book in Korea. Two rounds of radioligand receptor binding assays on natural products demonstrated that many plants show potent selectivity to various receptors, especially monoamine receptors presumed to be involved in mental disorders. Further studies for the pharmacologic activity of active ingredients and in vivo behavioral profiles focusing on the central nervous system (CNS) effects are currently under way with these plant extracts, These investigations may lead to the development of new psychotropic drugs with no serious side effects, a major concern for modern psychopharmacology. C1 NIMH,EXPTL THERAPEUT BRANCH,BETHESDA,MD. SEOUL NATL UNIV,SM,DEPT PSYCHIAT,SEOUL,SOUTH KOREA. KOREA ADV INST SCI & TECHNOL,GEN ENGN RES INST,TAEJON,SOUTH KOREA. RP CHUNG, IW (reprint author), CHUNGBUK NATL UNIV,COLL MED,DEPT NEUROPSYCHIAT,CHUNGBUK 360763,SOUTH KOREA. RI Chen, Guang/A-2570-2017 NR 29 TC 11 Z9 11 U1 1 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 139 EP 145 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500020 PM 7675978 ER PT J AU LEBOWITZ, BD MARTINEZ, RA NIEDEREHE, G PEARSON, JL REYNOLDS, CF RUDORFER, MV SCHNEIDER, LS KUPFER, DJ AF LEBOWITZ, BD MARTINEZ, RA NIEDEREHE, G PEARSON, JL REYNOLDS, CF RUDORFER, MV SCHNEIDER, LS KUPFER, DJ TI TREATMENT OF DEPRESSION IN LATE-LIFE SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material DE GERIATRIC; DEPRESSION; CLINICAL TRIALS; METHODOLOGY ID DOUBLE-BLIND; SCALE; SUICIDE; IMPACT; STRESS C1 UNIV PITTSBURGH,PITTSBURGH,PA. UNIV SO CALIF,LOS ANGELES,CA. RP LEBOWITZ, BD (reprint author), NIMH,MENTAL DISORDERS AGING RES BRANCH,ROOM 18-105,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 56 TC 9 Z9 9 U1 5 U2 6 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 1 BP 185 EP 202 PG 18 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RN615 UT WOS:A1995RN61500025 PM 7675983 ER PT J AU RUDORFER, MV AF RUDORFER, MV TI 1994 ANNUAL NCDEU MEETING WORKSHOPS AND SYMPOSIA SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material RP RUDORFER, MV (reprint author), NIMH,ROCKVILLE,MD 20857, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 2 BP 203 EP 204 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TF595 UT WOS:A1995TF59500001 ER PT J AU FLEMING, K BIGELOW, LB WEINBERGER, DR GOLDBERG, TE AF FLEMING, K BIGELOW, LB WEINBERGER, DR GOLDBERG, TE TI NEUROPSYCHOLOGICAL EFFECTS OF AMPHETAMINE MAY CORRELATE WITH PERSONALITY-CHARACTERISTICS SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article AB Although the stimulant properties of amphetamine are well established, its effects on cognitive test performance in unfatigued normal adults are poorly documented, Seventeen healthy individuals received a single oral dose of dextroamphetamine (0.25 mg/kg) in a double-blind, placebo-controlled, crossover study, Neurocognitive tests, motor tests, and behavioral observations were performed, Personality information, based on the Tridimensional Personality Questionnaire (TPQ) was also gathered to explore a relationship between personality factors and response to the stimulant, With the exception of two measures of reaction time, there were no overall changes in performance on measures of memory or other cognitive functions, There was decreased reaction time on the continuous performance task (CPT) and increased accuracy of performance under minimal delay conditions in the spatial delay response task while subjects were receiving amphetamine, In addition, the novelty-seeking subscale was found to correlate with a measure of verbal memory, Individuals with higher scores on the novelty-seeking scale deteriorated under amphetamine, while those who had lower scores improved, These results suggest that some cognitive abilities of persons who may have relatively high dopaminergic tone are disrupted by amphetamine, while those with relatively low dopaminergic tone may have their performance enhanced. C1 NIMH,ST ELIZABETHS HOSP,CTR NEUROSCI,CLIN BRAIN DISORDERS BRANCH,INTRAMURAL RES PROGRAM,WASHINGTON,DC 20032. UNIV CALIF IRVINE,IRVINE,CA 92717. NR 25 TC 36 Z9 36 U1 3 U2 5 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 2 BP 357 EP 362 PG 6 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TF595 UT WOS:A1995TF59500025 PM 7491392 ER PT J AU BITRAN, JA MANJI, HK POTTER, WZ GUSOVSKY, F AF BITRAN, JA MANJI, HK POTTER, WZ GUSOVSKY, F TI DOWN-REGULATION OF PKC-ALPHA BY LITHIUM IN-VITRO SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article ID PROTEIN-KINASE-C; INOSITOL PHOSPHOLIPID HYDROLYSIS; RAT-BRAIN; CELLS; AGONIST; HIPPOCAMPUS; CORTEX AB In recent years, research aimed at the elucidation of lithium's molecular mechanisms has focused on signal transduction pathways, This research has demonstrated that lithium has multiple effects on the phosphoinositide turnover signaling system, We have previously demonstrated that chronic (but not acute) in vitro exposure of HL60 cells to 1 mM lithium reduces both receptor and phorbol-ester-mediated Na+/H+ activity without affecting agonist-induced increases in intracellular Ca2+ or phosphoinositide breakdown, findings which suggest an attenuation of protein kinase C (PKC) function, The present study sought to measure the in vitro effects of lithium an PKC more directly and demonstrated that 5-day in vitro exposure of HL60 cells to either 1 mM or 10 mM lithium chloride dramatically reduces PKC alpha in both cytosolic and membrane fractions, Given the critical role of PKC in regulating neuronal signal transduction, these effects may play a major role in lithium's mood-stabilizing effects. C1 NIMH,EXPTL THERAPEUT BRANCH,CLIN PHARMACOL SECT,BETHESDA,MD 20892. NIDDK,BIOORGAN CHEM LAB,BETHESDA,MD 20892. NR 29 TC 16 Z9 16 U1 0 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 2 BP 449 EP 452 PG 4 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TF595 UT WOS:A1995TF59500037 PM 7491404 ER PT J AU Rudorfer, MV AF Rudorfer, MV TI Untitled SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material RP Rudorfer, MV (reprint author), NIMH,ROCKVILLE,MD 20857, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 453 EP 455 PG 3 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300001 ER PT J AU Jobson, KO Potter, WZ AF Jobson, KO Potter, WZ TI International psychopharmacology algorithm project report - Introduction SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material DE algorithm; schizophrenia; bipolar manic-depressive illness; unipolar major depression; panic disorder with agoraphobia; obsessive-compulsive disorder; comorbid psychiatric disorders; neuroleptic malignant syndrome; psychotic depression C1 NIMH,INTRAMURAL RES PROGRAM,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. TENNESSEE PSYCHIAT & PSYCHOPHARMACOL CLIN,KNOXVILLE,TN. NR 0 TC 35 Z9 35 U1 0 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 457 EP 459 PG 3 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300002 PM 8668749 ER PT J AU Zarate, CA Daniel, DG Kinon, BJ Litman, RE Naber, D Pickar, D Sato, M AF Zarate, CA Daniel, DG Kinon, BJ Litman, RE Naber, D Pickar, D Sato, M TI International psychopharmacology algorithm project report .A. Algorithms for the treatment of schizophrenia SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article C1 MCLEAN HOSP,BELMONT,MA 02178. WASHINGTON CLIN RES CTR,FALLS CHURCH,VA. HILLSIDE HOSP,ALBERT EINSTEIN COLL MED,GLEN OAKS,NY 11004. NIMH,INTRAMURAL RES PROGRAM,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. UNIV MUNICH,W-8000 MUNICH,GERMANY. TOHOKU UNIV,SCH MED,SENDAI,MIYAGI 980,JAPAN. RP Zarate, CA (reprint author), HARVARD UNIV,SCH MED,BELMONT,MA 02178, USA. NR 0 TC 15 Z9 15 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 461 EP 467 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300003 PM 8668750 ER PT J AU Suppes, T Calabrese, JR Mitchell, PB Pazzaglia, PJ Potter, WZ Zarin, DA AF Suppes, T Calabrese, JR Mitchell, PB Pazzaglia, PJ Potter, WZ Zarin, DA TI International psychopharmacology algorithm project report .B. Algorithms for the treatment of bipolar, manic-depressive illness SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article C1 CASE WESTERN RESERVE UNIV,SCH MED,CLEVELAND,OH 44106. UNIV NEW S WALES,KENSINGTON,NSW 2033,AUSTRALIA. UNIV MISSISSIPPI,JACKSON,MS 39216. NIMH,INTRAMURAL RES PROGRAM,EXPTL THERAPEUT BRANCH,BETHESDA,MD 20892. AMER PSYCHIAT ASSOC,WASHINGTON,DC 20009. RP Suppes, T (reprint author), UNIV TEXAS,SW MED SCH,DALLAS,TX 75230, USA. NR 0 TC 30 Z9 30 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 469 EP 474 PG 6 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300004 PM 8668751 ER PT J AU Jobson, KO Davidson, JRT Lydiard, RB McCann, UD Pollack, MH Rosenbaum, JF AF Jobson, KO Davidson, JRT Lydiard, RB McCann, UD Pollack, MH Rosenbaum, JF TI International psychopharmacology algorithm project report .D. Algorithm for the treatment of panic disorder with agoraphobia SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article C1 DUKE UNIV,MED CTR,DURHAM,NC. MED UNIV S CAROLINA,CHARLESTON,SC 29425. NIMH,INTRAMURAL RES PROGRAM,BIOL PSYCHIAT BRANCH,UNIT ANXIETY & AFFECT DISORDERS,BETHESDA,MD 20892. MASSACHUSETTS GEN HOSP,CLIN PSYCHOPHARMACOL UNIT,BOSTON,MA 02114. RP Jobson, KO (reprint author), TENNESSEE PSYCHIAT & PSYCHOPHARMACOL CLIN,KNOXVILLE,TN, USA. NR 0 TC 15 Z9 15 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 483 EP 485 PG 3 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300006 PM 8668753 ER PT J AU Jefferson, JW Altemus, M Jenike, MA Pigott, TA Stein, DJ Griest, JH AF Jefferson, JW Altemus, M Jenike, MA Pigott, TA Stein, DJ Griest, JH TI International psychopharmacology algorithm project report .E. Algorithm for the treatment of obsessive-compulsive disorder (OCD) SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article C1 FDN HLTH RES & EDUC,MADISON,WI. NIMH,INTRAMURAL RES PROGRAM,CLIN NEUROPHARMACOL BRANCH,BETHESDA,MD 20892. MASSACHUSETTS GEN HOSP,BOSTON,MA. UNIV TEXAS,MED BRANCH,GALVESTON,TX 77550. UNIV STELLENBOSCH,TYGERBERG 7505,SOUTH AFRICA. RP Jefferson, JW (reprint author), UNIV WISCONSIN,MADISON,WI 53706, USA. RI Stein, Dan/A-1752-2008 OI Stein, Dan/0000-0001-7218-7810 NR 0 TC 10 Z9 11 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 487 EP 490 PG 4 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300007 PM 8668754 ER PT J AU Mitchell, PB AF Mitchell, PB TI Novel French antidepressants not available in the United States SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Review DE tianeptine; amineptine; minaprine; medifoxamine; modafinil ID CONTROLLED TRIAL; MINAPRINE; AMINEPTINE; TIANEPTINE; AMITRIPTYLINE; DEPRESSION; MODAFINIL; PHARMACOKINETICS; MONKEYS AB There is little awareness in the United States and other English-speaking countries of a number of novel antidepressant drugs that have recently been developed and marketed in France. This review focuses on tianeptine, amineptine, minaprine, medifoxamine, and modafinil-examining both their pharmacological actions and their clinical efficacies, Their potential for further research or marketing in the United States is discussed. C1 NIMH,CLIN PHARMACOL SECT,BETHESDA,MD 20892. UNIV NEW S WALES,SCH PSYCHIAT,SYDNEY,NSW,AUSTRALIA. OI Mitchell, Philip/0000-0002-7954-5235 NR 110 TC 5 Z9 5 U1 1 U2 1 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 3 BP 509 EP 519 PG 11 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA TR743 UT WOS:A1995TR74300009 PM 8668756 ER PT J AU Rudorfer, MV AF Rudorfer, MV TI 1995 NCDEU workshops and symposia - Editor's note SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material RP Rudorfer, MV (reprint author), NIMH,ROCKVILLE,MD 20857, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 637 EP 638 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800001 ER PT J AU Lebowitz, BD Pollock, BG Caligiuri, MP Kluger, A LaghrissiThode, F AF Lebowitz, BD Pollock, BG Caligiuri, MP Kluger, A LaghrissiThode, F TI Instrumental measures in geriatric psychopharmacology SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP New Clin Drug Evaluat Unit, NIMH DE geriatrics; measurement; psychophysiology; Alzheimer's disease; movement disorders ID ALZHEIMERS-DISEASE; HIP FRACTURE; SENILE DEMENTIA; RISK-FACTORS; SCHIZOPHRENIA; DOPAMINE; BALANCE; FALLS C1 UNIV PITTSBURGH,PITTSBURGH,PA. UNIV CALIF SAN DIEGO,SAN DIEGO,CA 92103. NYU,NEW YORK,NY. RP Lebowitz, BD (reprint author), NIMH,AGING RES BRANCH,5600 FISHERS LANE,ROOM 18-101,ROCKVILLE,MD 20857, USA. FU NIMH NIH HHS [MH-45959, MH-52247, MH-01040] NR 55 TC 1 Z9 1 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 641 EP 649 PG 9 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800002 PM 8851635 ER PT J AU Allen, JP Litten, RZ Fertig, JB AF Allen, JP Litten, RZ Fertig, JB TI Strategies for treatment of alcohol problems SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP New Clin Drug Evaluat Unit, NIMH DE alcoholism treatment effectiveness; brief intervention; behavior contracting; naltrexone; social skills training; community reinforcement approach; patient-treatment matching ID COPING SKILLS; INTERACTIONAL THERAPIES; MATCHING ALCOHOLICS AB Well-controlled clinical trials have been performed to determine efficacy of alternative alcoholism treatment strategies, Six effective treatment approaches are discussed, and a research project example demonstrates the benefits of each, The treatments considered are: brief intervention, behavioral contracting, naltrexone, social skills training, the community reinforcement approach, and patient-treatment matching. RP Allen, JP (reprint author), NIAAA,TREATMENT RES BRANCH,WILLCO BLDG,SUITE 505,6000 EXECUT BLVD,MSC 7003,BETHESDA,MD 20892, USA. NR 19 TC 8 Z9 8 U1 1 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 665 EP 669 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800005 PM 8851638 ER PT J AU Li, SH Chiang, CN Tai, BC Marschke, CK Hawks, RL AF Li, SH Chiang, CN Tai, BC Marschke, CK Hawks, RL TI Quantitative versus qualitative urinalysis for benzoylecgonine in clinical trials for the assessment of cocaine use SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP New Clin Drug Evaluat Unit, NIMH DE cocaine; benzoylecgonine; clinical efficacy; qualitative urinalysis; quantitative urinalysis ID URINARY-EXCRETION; HUMANS AB Urinalysis of benzoylecgonine (BE) concentrations is a primary outcome measure for evaluating medications for treating cocaine addiction. Using simulated BE data from a set of simple clinical models, the advantages of quantitative versus qualitative urinalysis were evaluated, as well as the advantages of once-weekly versus thrice-weekly sampling schedules. A 60 percent reduction in cocaine use, either in daily amount or in weekly frequency, was considered clinically significant. Quantitative urinalysis can detect reductions in both amount and frequency, whereas qualitative urinalysis can detect only a decrease in frequency. For quantitative urinalysis, changes are more easily detected when urine is collected three times a week than when it is collected once a week. For qualitative urinalysis, the majority rule analysis for a thrice-weekly sampling schedule yields an artificially high estimate of the percentage of positive samples, whereas a once-weekly schedule gives a highly variable estimate. RP Li, SH (reprint author), NIDA,NIH,ROOM 11A-55,PARKLAWN BLDG,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 16 TC 9 Z9 9 U1 0 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 671 EP 679 PG 9 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800006 PM 8851639 ER PT J AU Blehar, VC AF Blehar, VC TI Gender differences in risk factors for mood and anxiety disorders: Implications for clinical treatment research - Introduction SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Editorial Material CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP NIMH, New Clin Drug Evaluat Unit DE gender differences; sex differences; mood disorders; anxiety disorders; affective disorders ID AGE; SEX AB This article discusses the utility and treatment implications of a focus on gender-related variables in the study of the mood and anxiety disorders. Three clinical areas are considered: (1) Mood and anxiety disorders during female reproductive transitions; (2) gender differences in pathogenesis and pathopsysiology; and (3) gender differences in clinical phenomenology and course, Gender analysis is viewed as useful in specifying variability in treatment response, especially in regard to the menstrual cycle, pregnancy, the postpartum period, and menopause; and in defining clinical covariates modifying treatment course. A psychobiological approach to mood and anxiety disorders is seen as critical in this regard. RP Blehar, VC (reprint author), NIMH, NIH, ROOM 10C-06, 5600 FISHERS LANE, ROCKVILLE, MD 20857 USA. NR 30 TC 11 Z9 11 U1 9 U2 9 PU MEDWORKS MEDIA GLOBAL, LLC PI HERMOS BEACH PA 670 FIFTH STREET, STE A, HERMOS BEACH, CA 90254 USA SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 687 EP 691 PG 5 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800008 ER PT J AU Leibenluft, E Turner, EH FeldmanNaim, S Schwartz, PJ Wehr, TA Rosenthal, NE AF Leibenluft, E Turner, EH FeldmanNaim, S Schwartz, PJ Wehr, TA Rosenthal, NE TI Light therapy in patients with rapid cycling bipolar disorder: Preliminary results SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP New Clin Drug Evaluat Unit, NIMH DE bipolar disorder; light therapy; circadian rhythms; rapid cycling; melatonin ID BRIGHT-LIGHT; PHOTOTHERAPY; DEPRESSION; MANIA; VALPROATE; LITHIUM AB Nine patients with rapid cycling bipolar disorder were treated with a total of 13 trials of bright light therapy in the morning (n=5), evening (n=3), or midday (n=5). In each instance, the patient's mood ratings during 3 months of light therapy (added to a stable medication regimen) were compared to his or her mood ratings during 3 months on the same medication but without light treatment. Of the 3 light therapy schedules, only midday lights appeared to have beneficial clinical effects, improving mood ratings in 3 patients. In contrast, the morning light therapy trial was terminated prematurely in 3 patients because of clinical instability. Light treatment was better tolerated if patients discontinued it on days when they were hypomanic. The clinical and theoretical implications of these preliminary findings are discussed. RP Leibenluft, E (reprint author), NIMH,CLIN PSYCHOBIOL BRANCH,BLDG 10,ROOM 4S-239,10 CTR DR MSC 1390,BETHESDA,MD 20892, USA. RI Turner, Erick/A-4848-2008 OI Turner, Erick/0000-0002-3522-3357 NR 35 TC 45 Z9 45 U1 0 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 705 EP 710 PG 6 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800010 PM 8851643 ER PT J AU Cott, J AF Cott, J TI Natural product formulations available in Europe for psychotropic indications SO PSYCHOPHARMACOLOGY BULLETIN LA English DT Article; Proceedings Paper CT 35th Annual New-Clinical-Drug-Evaluation-Unit Meeting, of the National-Institute-of-Mental-Health CY MAY 30-JUN 03, 1995 CL ORLANDO, FL SP New Clin Drug Evaluat Unit, NIMH DE botanical medicine; herbal medicine; ginkgo; valerian; ginseng; hypericum ID GINKGO-BILOBA EXTRACT; DOUBLE-BLIND; PLACEBO; EGB-761 AB Until the middle of this century, development of medical treatment for human disease was intimately connected with the plant kingdom. Despite advances of the last three decades in utilizing chemical synthetic approaches to drug design and sophisticated structure-activity studies, there is still a great need for novel compounds with unique mechanisms of action in the field of medicine. While many thousands of structural analogs have been synthesized and tested, numerous gaps remain in the therapeutic armamentarium for psychiatric illnesses. Most new drugs marketed for psychotherapeutic indications in recent years have been only incremental improvements on existing medications. Major breakthroughs have resulted primarily from the study of natural products. Some of our most valuable drugs have been isolated from plant and animal sources, including aspirin, morphine, reserpine (the first antipsychotic), almost all of our antibiotics, digitalis, and such anti-cancer agents as vincristine, vinblastine, and taxol. Recent political and social events suggest that new emphasis will be placed on natural products research in the years to come. This article highlights therapeutic applications of Ginkgo biloba, Hypericum perforatum, Valerian officinalis, and Panex ginseng. RP Cott, J (reprint author), NIMH,DIV CLIN & TREATMENT RES,PHARMACOL TREATMENT RES PROGRAM,5600 FISHERS LANE,ROOM 18-105,ROCKVILLE,MD 20857, USA. NR 45 TC 33 Z9 35 U1 1 U2 4 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0048-5764 J9 PSYCHOPHARMACOL BULL JI Psychopharmacol. Bull. PY 1995 VL 31 IS 4 BP 745 EP 751 PG 7 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA UB038 UT WOS:A1995UB03800015 PM 8851648 ER PT J AU FREEDLAND, KE CAMEY, RM FORMAN, S KNATTERUD, GL KAUFMANN, PG KETTERER, MW KRANTZ, DS LIGHT, K LINDHOLM, L RACZYNSKI, J STONE, P AF FREEDLAND, KE CAMEY, RM FORMAN, S KNATTERUD, GL KAUFMANN, PG KETTERER, MW KRANTZ, DS LIGHT, K LINDHOLM, L RACZYNSKI, J STONE, P TI PSYCHOLOGICAL CORRELATES OF ANGINAL SYMPTOMS DURING POSITIVE EXERCISE TREADMILL TESTS SO PSYCHOSOMATIC MEDICINE LA English DT Meeting Abstract C1 WASHINGTON UNIV,ST LOUIS,MO. MARYLAND MED RES INST,BALTIMORE,MD. NHLBI,BETHESDA,MD. HENRY FORD HOSP,DETROIT,MI. UNIFORMED SERV UNIV HLTH SCI,BETHESDA,MD. UNIV N CAROLINA,CHAPEL HILL,NC 27599. UNIV FLORIDA,GAINESVILLE,FL 32611. UNIV ALABAMA,TUSCALOOSA,AL 35487. BRIGHAM & WOMENS HOSP,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0033-3174 J9 PSYCHOSOM MED JI Psychosom. Med. PD JAN-FEB PY 1995 VL 57 IS 1 BP 74 EP 74 PG 1 WC Psychiatry; Psychology; Psychology, Multidisciplinary SC Psychiatry; Psychology GA QE074 UT WOS:A1995QE07400080 ER PT J AU HAVAS, S HEIMENDINGER, J DAMRON, D NICKLAS, TA COWAN, A BERESFORD, SAA SORENSEN, G BULLER, D BISHOP, D BARANOWSKI, T REYNOLDS, K AF HAVAS, S HEIMENDINGER, J DAMRON, D NICKLAS, TA COWAN, A BERESFORD, SAA SORENSEN, G BULLER, D BISHOP, D BARANOWSKI, T REYNOLDS, K TI 5-A-DAY FOR BETTER HEALTH - 9 COMMUNITY RESEARCH PROJECTS TO INCREASE FRUIT AND VEGETABLE CONSUMPTION SO PUBLIC HEALTH REPORTS LA English DT Article ID CANCER RISK; MASS-MEDIA; PREVENTION; PROMOTION; EDUCATION; CAMPAIGN; DISEASE AB One of the national objectives in ''Healthy People 2000'' is for members of the public to increase their consumption of fruits and vegetables to five or more servings daily. The National Cancer Institute (NCI) began a nationwide campaign to achieve this objective in 1991. As part of this campaign, the NCI funded nine research studies in 1993. These projects are implementing and evaluating community-based programs designed to increase fruit and vegetable consumption among different segments of the population in Alabama, Arizona, Georgia, Louisiana, Maryland, Massachusetts, Minnesota, North Carolina, and Washington. The settings of these projects include the Special Supplement Food Program for Women, Infants, and Children (WIC Programs), churches, worksites, and schools. The projects are led by multidisciplinary teams and entail extensive collaboration among academic, governmental, private sector, and voluntary agencies within each State. The projects represent a model public health paradigm for conducting this type of research. C1 NCI,5-A-DAY BETTER HLTH,BALTIMORE,MD 21201. TULANE UNIV,SCH PUBL HLTH & TROP MED,DEPT NUTR,NEW ORLEANS,LA 70118. N CAROLINA DEPT ENVIRONM HLTH & NAT RESOURCES,NUTR BRANCH,CHARLOTTE,NC. UNIV WASHINGTON,DEPT EPIDEMIOL,SEATTLE,WA 98195. HARVARD UNIV,SCH PUBL HLTH,DEPT HLTH & SOCIAL BEHAV,BOSTON,MA 02115. UNIV ARIZONA,SCH MED,DEPT COMMUN,TUCSON,AZ 85721. MINNESOTA DEPT HLTH,HLTH BEHAV DEV & EDUC SECT,MINNEAPOLIS,MN. EMORY UNIV,SCH PUBL HLTH,DEPT BEHAV SCI HLTH EDUC,ATLANTA,GA 30322. UNIV ALABAMA,SCH PUBL HLTH,DEPT HLTH BEHAV,BIRMINGHAM,AL 35233. RP HAVAS, S (reprint author), UNIV MARYLAND,SCH MED,DEPT EPIDEMIOL & PREVENT MED,655 W BALTIMORE ST,BALTIMORE,MD 21201, USA. NR 29 TC 108 Z9 108 U1 1 U2 3 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0033-3549 J9 PUBLIC HEALTH REP JI Public Health Rep. PD JAN-FEB PY 1995 VL 110 IS 1 BP 68 EP 79 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA QG127 UT WOS:A1995QG12700012 PM 7838947 ER PT B AU GESELOWITZ, DA MCMANAWAY, MM HOFER, KG NEUMANN, R AF GESELOWITZ, DA MCMANAWAY, MM HOFER, KG NEUMANN, R BE Fuciarelli, AF Zimbrick, JD TI Low-let radiotoxicity from incorporated H-3 or I-125 during the cell cycle: Implications for mechanisms of high-LET damage SO RADIATION DAMAGE IN DNA: STRUCTURE/FUNCTION RELATIONSHIPS AT EARLY TIMES LA English DT Proceedings Paper CT International Workshop on Radiation Damage in DNA - Structure/Function Relationships at Early Times CY OCT 01-06, 1994 CL GLENEDEN BEACH, OR SP Rad Res Soc, Dept Energy, NCI, Battelle, Pacific NW Labs, Life Sci Ctr, Beckman Instruments Inc, Hewlett Packard Inc C1 NIH,CTR CLIN,DEPT NUCL MED,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BATTELLE PRESS PI COLUMBUS PA 505 KING AVE, COLUMBUS, OH 43201 BN 0-935470-90-5 PY 1995 BP 223 EP 230 PG 8 WC Biochemistry & Molecular Biology; Biophysics; Chemistry, Medicinal; Chemistry, Organic; Physics, Condensed Matter; Radiology, Nuclear Medicine & Medical Imaging SC Biochemistry & Molecular Biology; Biophysics; Pharmacology & Pharmacy; Chemistry; Physics; Radiology, Nuclear Medicine & Medical Imaging GA BE26M UT WOS:A1995BE26M00023 ER PT J AU LAND, CE AF LAND, CE TI DO GAMMA-RAYS AND ALPHA-PARTICLES CAUSE DIFFERENT TYPES OF LUNG-CANCER - A COMPARISON BETWEEN ATOMIC-BOMB SURVIVORS AND URANIUM MINERS SO RADIATION PROTECTION DOSIMETRY LA English DT Article; Proceedings Paper CT Symposium on Radiobiology and Dosimetry of Inhaled Radionuclides CY NOV 09-10, 1993 CL PACIFIC NW LAB, RICHLAND, WA HO PACIFIC NW LAB AB Excess lung cancer risk has been associated with exposure to alpha particle radiation from inhaled radon daughter products among uranium miners in Czechoslovakia, Canada, the United States, and elsewhere, and with exposure to gamma rays and neutrons from the atomic bombings of Hiroshima and Nagasaki, Japan. Differences in distribution by histological type, as well as certain epidemiological differences, suggest the possibility of differences in the causation of radiation-induced lung cancer. An epidemiological analysis is summarised of results from a blind pathology panel review of tissue slides from lung cancer cases diagnosed in 108 Japanese A bomb survivors and 92 American uranium miners selected on the basis of radiation exposure, smoking history, sex, age, and source and quality of pathology material. Consensus diagnoses were obtained with respect to principal sub-type, including squamous cell cancer, small cell cancer, adenocarcinoma, and less frequent sub-types. The results were analysed in terms of population, radiation dose, and smoking history. As expected, the proportion of squamous cell cancer was positively related to smoking history in both populations. The relative frequencies of small cell cancer and adenocarcinoma were very different in the two populations, but this difference was adequately accounted for by differences in radiation dose (more specifically, dose-based relative risk estimates based on published risk coefficients). Data for the two populations conformed to a common pattern, in which radiation-induced cancers appeared more likely to be of the small-cell sub-type, and less likely to be adenocarcinomas. No additional explanation in terms of radiation quality (alpha particles or gamma rays), uniform or local irradiation, inhaled as against external radiation source, or other population differences, appeared to be required. One possible interpretation of the finding is that radiation-related lung carcinogenesis may depend heavily on interactions with inhaled promoter/progressor agents, such as smoke. Thus, even though ionising events from external gamma rays and inhaled radon decay products are very differently distributed within the lung, the spatial distribution, and cell types, of any resulting cancers may be determined largely by the action of other agents deposited within the bronchi. RP LAND, CE (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,6130 EXECUT BLVD,ROOM 408,BETHESDA,MD 20853, USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU NUCLEAR TECHNOLOGY PUBL PI ASHFORD PA PO BOX 7, ASHFORD, KENT, ENGLAND TN23 1YW SN 0144-8420 J9 RADIAT PROT DOSIM JI Radiat. Prot. Dosim. PY 1995 VL 60 IS 4 BP 279 EP 285 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA TA948 UT WOS:A1995TA94800006 ER PT J AU BOUVILLE, A AF BOUVILLE, A TI THE CHERNOBYL ACCIDENT SO RADIATION PROTECTION DOSIMETRY LA English DT Article; Proceedings Paper CT Symposium on Radiobiology and Dosimetry of Inhaled Radionuclides CY NOV 09-10, 1993 CL PACIFIC NW LAB, RICHLAND, WA HO PACIFIC NW LAB AB The accident at Unit 4 of the Chernobyl nuclear power plant, which is located in Ukraine, about 30 km south of the border with Belarus, was the most severe in the nuclear industry. The accident, which occurred in April 1986, caused the rapid death of 31 power plant employees and firemen, mainly from acute radiation exposures and burns, and brought about the evacuation of 116,000 people within a few weeks. In addition, about half a million workers and four million members of the public have been exposed, to some extent, to radiation doses resulting from the Chernobyl accident. A large number of radiation measurements have been made since the accident in order to reconstruct the doses received by the most exposed populations. On the basis of currently available information, it appears that: (1) average doses received by clean-up workers from external irradiation decreased with time, being about 300 mGy for the persons who worked in the first three months after the accident, about 170 mGy for the remainder of 1986, 130 mGy in 1987, 30 mGy in 1988, and 15 mGy in 1989; (2) the evacuees received, before evacuation, effective doses averaging 11 mSv for the population of Pripyat, and 18 mSv for the remainder of the population of the 30 km zone, with maximum effective doses ranging up to 380 mSv; and (3) among the populations living in contaminated areas, the highest doses were those delivered to the thyroids of children. Thyroid doses derived from thyroid measurements among Belarussian and Ukrainian children indicate median thyroid doses of about 300 mGy, and more than 1% of the children with thyroid doses in excess of 5000 mGy. A description is provided of the epidemiological studies that the National Cancer Institute has, since 1990, at the request of the Department of Energy endeavoured to undertake, in cooperation with Belarus and Ukraine, on two possible health effects resulting from the Chernobyl accident: (1) thyroid cancer in children living in contaminated areas during the first few weeks following the accident, and (2) leukaemia among workers involved in clean-up operations at the reactor site in 1986 and 1987. RP BOUVILLE, A (reprint author), NCI,RADIAT EFFECTS BRANCH,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 3 U2 9 PU NUCLEAR TECHNOLOGY PUBL PI ASHFORD PA PO BOX 7, ASHFORD, KENT, ENGLAND TN23 1YW SN 0144-8420 J9 RADIAT PROT DOSIM JI Radiat. Prot. Dosim. PY 1995 VL 60 IS 4 BP 287 EP 293 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA TA948 UT WOS:A1995TA94800007 ER PT J AU LUBIN, JH STEINDORF, K AF LUBIN, JH STEINDORF, K TI CIGARETTE USE AND THE ESTIMATION OF LUNG-CANCER ATTRIBUTABLE TO RADON IN THE UNITED-STATES SO RADIATION RESEARCH LA English DT Article ID URANIUM MINERS; MORTALITY AB Residential exposure to radioactive radon and its decay products has been estimated to account for 10-12% of all lung cancer deaths in the U.S. It has been difficult to evaluate fully the impact of cigarette smoking, the most important cause of lung cancer, on this estimate, because factors for patterns of tobacco use have not been included in the risk models, since risk models are derived from studies of underground miners exposed to radon and detailed data on smoking are limited. Lung cancer risk estimates for exposure to radon progeny in smoker and nonsmoker populations are obtained by applying the same risk model to each population group, thereby assuming the joint effects of smoking and exposure to radon progeny are multiplicative. However, in miners, joint relative risks (RR) for the two exposures are most consistent with an intermediate relationship between multiplicative and additive, so that the present approach likely results in an overestimate of risk in smokers and an underestimate of risk in nonsmokers. Lubin et al. (National Institutes of Health Publication No. 94-3644, 1994) present an ad hoc approach for adjusting risk models to incorporate smoking status. The approach is based on the relative magnitude of the effects of radon progeny in smokers and nonsmokers and therefore may not be applicable to non-miner populations if the proportion of smokers and the RR for smoking differ. We show that the modification can be derived explicitly by assuming an arithmetic mixture model for the joint RR for smoking and exposure to radon progeny. In this way, smoking parameters in the population of interest (the proportion of smokers and the RR of smoking) can be used directly to adjust radon progeny risk models and obtain risk estimates that are specific for smokers and nonsmokers. With an intermediate RR relationship for smoking and radon progeny, the attributable percentage of lung cancer deaths from residential radon may be twofold greater in nonsmokers than in smokers. C1 GERMAN CANC RES CTR,DIV EPIDEMIOL,W-6900 HEIDELBERG,GERMANY. RP LUBIN, JH (reprint author), NCI,EPIDEMIOL METHODS SECT,EXECUT PLAZA N,ROOM 403,BETHESDA,MD 20892, USA. NR 20 TC 24 Z9 26 U1 0 U2 0 PU RADIATION RESEARCH SOC PI OAK BROOK PA 2021 SPRING RD, STE 600, OAK BROOK, IL 60521 SN 0033-7587 J9 RADIAT RES JI Radiat. Res. PD JAN PY 1995 VL 141 IS 1 BP 79 EP 85 DI 10.2307/3579093 PG 7 WC Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Radiology, Nuclear Medicine & Medical Imaging GA PZ205 UT WOS:A1995PZ20500012 PM 7997518 ER PT J AU RON, E PRESTON, DL MABUCHI, K AF RON, E PRESTON, DL MABUCHI, K TI MORE ABOUT CANCER INCIDENCE IN ATOMIC-BOMB SURVIVORS - SOLID TUMORS, 1958-1987 SO RADIATION RESEARCH LA English DT Letter C1 RADIAT EFFECTS RES FDN,DEPT STAT,HIROSHIMA 732,JAPAN. RADIAT EFFECTS RES FDN,DEPT EPIDEMIOL,HIROSHIMA 732,JAPAN. RP RON, E (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,BETHESDA,MD 20892, USA. NR 3 TC 7 Z9 7 U1 0 U2 0 PU RADIATION RESEARCH SOC PI OAK BROOK PA 2021 SPRING RD, STE 600, OAK BROOK, IL 60521 SN 0033-7587 J9 RADIAT RES JI Radiat. Res. PD JAN PY 1995 VL 141 IS 1 BP 126 EP 127 DI 10.2307/3579102 PG 2 WC Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Radiology, Nuclear Medicine & Medical Imaging GA PZ205 UT WOS:A1995PZ20500022 PM 7997509 ER PT B AU Resnick, M Bennett, C Kouprina, N Larionov, V Lewis, K Tran, H AF Resnick, M Bennett, C Kouprina, N Larionov, V Lewis, K Tran, H BE Hagen, U Harder, D Jung, H Streffer, C TI Recombinational repair of double-strand breaks: Twenty years later SO RADIATION RESEARCH 1895-1995, CONGRESS PROCEEDINGS LA English DT Proceedings Paper CT 10th International Congress of Radiation Research (10th ICRR) - Radiation Research 1895-1995 CY AUG 27-SEP 01, 1995 CL WURZBURG, GERMANY SP Dtsche Rontgengesell, Dtsche Gesell Biophys, Gemeinschaftsausschuss Strahlenforsch, Int Assoc Radiat Res RP Resnick, M (reprint author), NIEHS,NIH,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU 10TH ICRR SOCIETY (INT CONGRESS RADIATION RES) PI WURZBURG PA WURZBURG, GERMANY BN 3-00-000842-X PY 1995 BP 362 EP 367 PG 6 WC Physics, Atomic, Molecular & Chemical; Radiology, Nuclear Medicine & Medical Imaging SC Physics; Radiology, Nuclear Medicine & Medical Imaging GA BH47W UT WOS:A1995BH47W00071 ER PT B AU Land, C AF Land, C BE Hagen, U Harder, D Jung, H Streffer, C TI Some epidemiological approaches to radiation-related cancer risk SO RADIATION RESEARCH 1895-1995, CONGRESS PROCEEDINGS LA English DT Proceedings Paper CT 10th International Congress of Radiation Research (10th ICRR) - Radiation Research 1895-1995 CY AUG 27-SEP 01, 1995 CL WURZBURG, GERMANY SP Dtsche Rontgengesell, Dtsche Gesell Biophys, Gemeinschaftsausschuss Strahlenforsch, Int Assoc Radiat Res RP Land, C (reprint author), NCI,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU 10TH ICRR SOCIETY (INT CONGRESS RADIATION RES) PI WURZBURG PA WURZBURG, GERMANY BN 3-00-000842-X PY 1995 BP 1134 EP 1137 PG 4 WC Physics, Atomic, Molecular & Chemical; Radiology, Nuclear Medicine & Medical Imaging SC Physics; Radiology, Nuclear Medicine & Medical Imaging GA BH47W UT WOS:A1995BH47W00240 ER PT B AU Boice, JD AF Boice, JD BE Hagen, U Harder, D Jung, H Streffer, C TI Comparing low-level radiation with other hazards SO RADIATION RESEARCH 1895-1995, CONGRESS PROCEEDINGS LA English DT Proceedings Paper CT 10th International Congress of Radiation Research (10th ICRR) - Radiation Research 1895-1995 CY AUG 27-SEP 01, 1995 CL WURZBURG, GERMANY SP Dtsche Rontgengesell, Dtsche Gesell Biophys, Gemeinschaftsausschuss Strahlenforsch, Int Assoc Radiat Res RP Boice, JD (reprint author), NCI,RADIAT EPIDEMIOL BRANCH,BETHESDA,MD 20852, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU 10TH ICRR SOCIETY (INT CONGRESS RADIATION RES) PI WURZBURG PA WURZBURG, GERMANY BN 3-00-000842-X PY 1995 BP 1199 EP 1202 PG 4 WC Physics, Atomic, Molecular & Chemical; Radiology, Nuclear Medicine & Medical Imaging SC Physics; Radiology, Nuclear Medicine & Medical Imaging GA BH47W UT WOS:A1995BH47W00256 ER PT J AU BIZZI, A MOVSAS, B TEDESCHI, G PHILLIPS, CL OKUNIEFF, P ALGER, JR DICHIRO, G AF BIZZI, A MOVSAS, B TEDESCHI, G PHILLIPS, CL OKUNIEFF, P ALGER, JR DICHIRO, G TI RESPONSE OF NON-HODGKIN-LYMPHOMA TO RADIATION-THERAPY - EARLY AND LONG-TERM ASSESSMENT WITH H-1 MR SPECTROSCOPIC IMAGING SO RADIOLOGY LA English DT Article DE BRAIN NEOPLASMS, EMISSION CT; BRAIN NEOPLASMS, SECONDARY; HODGKIN DISEASE, THERAPEUTIC RADIOLOGY; MAGNETIC RESONANCE (MR), SPECTROSCOPY ID MAGNETIC-RESONANCE; HUMAN BRAIN; TUMORS; TOMOGRAPHY; SPECTRUM; CELLS AB PURPOSE: To determine whether proton magnetic resonance (MR) spectroscopic imaging is a sensitive technique for defining tumor extent and assessing response of cerebral lymphoma td therapy. MATERIALS AND METHODS: Four hydrogen-1 MR spectroscopic imaging studies were performed in a patient with non-Hodgkin lymphoma (NHL) before, during, and after radiation therapy and at follow-up at 33 months after diagnosis of the recurrence of disease: The patient had a single, large lesion in the brain and underwent hyperfractionated radiation therapy for 4 weeks. A series of MR images was also obtained. RESULTS: The pretreatment study showed a lesion with a distinct spectral pattern: marked elevation of choline and lipids and great reduction of creatine and N-acetylaspartate. During and after treatment, H-1 MR spectroscopic images depicted a progressive reduction of the abnormal choline and lipid signals. Thirty-three months after treatment, multiple-section H-1 MR spectroscopic images showed normal spectral patterns in all sections examined. CONCLUSION: H-1 MR spectroscopy may contribute to the neuroradiologic evaluation of NHL and, in particular, maybe useful in monitoring therapeutic response. C1 NINCDS,NEUROIMAGING BRANCH,BETHESDA,MD 20892. JOHNS HOPKINS UNIV,DEPT RADIOL & RADIOL SCI,BALTIMORE,MD. NCI,RADIAT ONCOL BRANCH,BETHESDA,MD 20892. NCI,DEPT PATHOL,BETHESDA,MD 20892. RI Bizzi, Alberto/K-3141-2016; OI Bizzi, Alberto/0000-0002-0253-5274; Phillips, Carrie/0000-0002-0256-0736 NR 23 TC 57 Z9 66 U1 0 U2 1 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD JAN PY 1995 VL 194 IS 1 BP 271 EP 276 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA PX684 UT WOS:A1995PX68400051 PM 7997566 ER PT S AU STOJILKOVIC, SS CATT, KJ AF STOJILKOVIC, SS CATT, KJ BE Bardin, CW TI EXPRESSION AND SIGNAL-TRANSDUCTION PATHWAYS OF GONADOTROPIN-RELEASING-HORMONE RECEPTORS SO RECENT PROGRESS IN HORMONE RESEARCH, VOL 50: PROCEEDINGS OF THE 1993 LAURENTIAN HORMONE CONFERENCE SE RECENT PROGRESS IN HORMONE RESEARCH LA English DT Review CT 1993 Laurentian Hormone Conference CY 1993 CL PR SP Endocrine Soc ID PROTEIN-KINASE-C; RAT ANTERIOR-PITUITARY; MESSENGER-RIBONUCLEIC-ACID; CYTOSOLIC FREE CALCIUM; GNRH BINDING-SITES; CANCER CELL-LINES; STIMULATES PHOSPHATIDYLINOSITOL METABOLISM; INOSITOL PHOSPHATE PRODUCTION; PANCREATIC ACINAR-CELLS; ALPHA-FACTOR RECEPTOR RP STOJILKOVIC, SS (reprint author), NICHHD, ENDOCRINOL & REPROD RES BRANCH, BETHESDA, MD 20892 USA. NR 277 TC 130 Z9 131 U1 1 U2 1 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0079-9963 BN 0-12-571150-6 J9 RECENT PROG HORM RES PY 1995 VL 50 BP 161 EP 205 PG 45 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Reproductive Biology SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Reproductive Biology GA BD34F UT WOS:A1995BD34F00008 PM 7740156 ER PT S AU NUNEZ, SB MEDIN, JA KELLER, H WANG, K OZATO, K WAHLI, W SEGARS, J AF NUNEZ, SB MEDIN, JA KELLER, H WANG, K OZATO, K WAHLI, W SEGARS, J BE Bardin, CW TI RETINOID-X RECEPTOR-BETA AND PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR ACTIVATE AN ESTROGEN RESPONSE ELEMENT SO RECENT PROGRESS IN HORMONE RESEARCH, VOL 50: PROCEEDINGS OF THE 1993 LAURENTIAN HORMONE CONFERENCE SE RECENT PROGRESS IN HORMONE RESEARCH LA English DT Note CT 1993 Laurentian Hormone Conference CY 1993 CL PR SP Endocrine Soc ID NUCLEAR HORMONE RECEPTORS; THYROID-HORMONE; SIGNALING PATHWAYS; RXR-BETA; GENE; BINDING; ACID; RAT; TRANSCRIPTION; EXPRESSION C1 NICHHD,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. NICHHD,MOLEC GROWTH REGULAT LAB,BETHESDA,MD 20892. UNIV LAUSANNE,INST BIOL ANIM,CH-1015 LAUSANNE,SWITZERLAND. RI Wahli, Walter/B-1398-2009 OI Wahli, Walter/0000-0002-5966-9089 NR 27 TC 15 Z9 15 U1 0 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0079-9963 BN 0-12-571150-6 J9 RECENT PROG HORM RES PY 1995 VL 50 BP 409 EP 416 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Reproductive Biology SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Reproductive Biology GA BD34F UT WOS:A1995BD34F00025 PM 7740175 ER PT S AU Samelson, LE Donovan, JA Isakov, N Ota, Y Wange, RL AF Samelson, LE Donovan, JA Isakov, N Ota, Y Wange, RL BE Naor, D DeMeyts, P Feldmann, M Schlessinger, J TI Signal transduction mediated by the T-cell antigen receptor SO RECEPTOR ACTIVATION BY ANTIGENS, CYTOKINES, HORMONES, AND GROWTH FACTORS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Receptor Activation by Antigens, Cytokines, Hormones, and Growth Factors CY OCT 21-25, 1994 CL ORLANDO, FL SP New York Acad Sci ID PROTEIN-TYROSINE KINASE; ZETA-CHAIN; CD4 RECEPTOR; V-CBL; ACTIVATION; PHOSPHORYLATION; ASSOCIATION; DOMAIN; COMPLEX; ZAP-70 RP Samelson, LE (reprint author), NICHHD,CELL BIOL & METAB BRANCH,BLDG 18T,ROOM 101,18 LIB DR,MSC 5430,BETHESDA,MD 20892, USA. NR 68 TC 17 Z9 17 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-951-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 766 BP 157 EP 172 DI 10.1111/j.1749-6632.1995.tb26659.x PG 16 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Immunology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Immunology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BE40J UT WOS:A1995BE40J00017 PM 7486652 ER PT S AU LeRoith, D Werner, H Neuenschwander, S Kalebic, T Helman, LJ AF LeRoith, D Werner, H Neuenschwander, S Kalebic, T Helman, LJ BE Naor, D DeMeyts, P Feldmann, M Schlessinger, J TI The role of the insulin-like growth factor-I receptor in cancer SO RECEPTOR ACTIVATION BY ANTIGENS, CYTOKINES, HORMONES, AND GROWTH FACTORS SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on Receptor Activation by Antigens, Cytokines, Hormones, and Growth Factors CY OCT 21-25, 1994 CL ORLANDO, FL SP New York Acad Sci ID PEPTIDE ANALOGS; WILMS-TUMORS; EXPRESSION; INHIBITION; IGF1R; GENE; MICE C1 NCI, PEDIAT BRANCH, MOLEC ONCOL SECT, BETHESDA, MD 20892 USA. RP NIDDKD, SECT MOLEC & CELLULAR PHYSIOL, DIABET BRANCH, BLDG 10, ROOM 8S-239, BETHESDA, MD 20892 USA. NR 30 TC 55 Z9 55 U1 1 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-952-5; 0-89766-951-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 766 BP 402 EP 408 DI 10.1111/j.1749-6632.1995.tb26689.x PG 7 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Immunology; Neurosciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism; Immunology; Neurosciences & Neurology; Pharmacology & Pharmacy GA BE40J UT WOS:A1995BE40J00047 PM 7486685 ER PT J AU GILON, P YAKEL, JL AF GILON, P YAKEL, JL TI ACTIVATION OF 5-HT3 RECEPTORS EXPRESSED IN XENOPUS OOCYTES DOES NOT INCREASE CYTOPLASMIC CA2+ LEVELS SO RECEPTORS & CHANNELS LA English DT Article DE SEROTONIN RECEPTORS; XENOPUS OOCYTE; ION CHANNEL ID INOSITOL TRISPHOSPHATE; NMDA RECEPTOR; CALCIUM PERMEABILITY; MOLECULAR-CLONING; DIVALENT-CATIONS; GANGLION NEURONS; CHANNELS; CELLS; PERMEATION; CURRENTS AB The wildtype 5-HT3 receptor was expressed in Xenopus oocytes from a cloned cDNA. Two-electrode voltage-clamp and fura-2 techniques were used simultaneously to monitor the current induced by the activation of the 5-HT3 receptor and the cytoplasmic Ca2+ concentration ([Ca2+](i)). The application of serotonin (5-HT; 50 mu M) in a physiological bathing solution containing Ca2+ (1.8 mM) elicited inward currents of up to similar to 1.3 mu A at a potential of -90 mV. There was little or no detectable change in [Ca2+](i). In experiments on oocytes bathing in a nominally Ca2+-free medium, the injection of (2,4,5)IP3, an analog of inositol 1,4,5-trisphosphate ((1,4,5)IP3), produced a large increase in [Ca2+](i) levels due to the liberation bf Ca2+ from intracellular stores. This response was followed by a sustained elevation of [Ca2+](i) upon restoring extracellular Ca2+ (i.e. capacitative Ca2+ entry). Furthermore, when calcium-permeable heteromeric (NR1/NR2A) N-methyl-D-aspartate (NMDA) receptors were expressed in oocytes, the activation of these receptors led to a large increase in [Ca2+](i) in a Ca2+-containing external medium. It is concluded that wildtype 5-HT3 receptors expressed in Xenopus oocytes and studied under conditions used here show little or no permeability for Ca2+. C1 NIEHS,CELLULAR & MOLEC PHARMACOL LAB,RES TRIANGLE PK,NC 27709. NR 28 TC 12 Z9 12 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1060-6823 J9 RECEPTOR CHANNEL JI Recept. Channels PY 1995 VL 3 IS 2 BP 83 EP 88 PG 6 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA RZ235 UT WOS:A1995RZ23500002 PM 8581403 ER PT J AU STADTMAN, ER AF STADTMAN, ER TI ROLE OF OXIDIZED AMINO-ACIDS IN PROTEIN BREAKDOWN AND STABILITY SO REDOX-ACTIVE AMINO ACIDS IN BIOLOGY SE METHODS IN ENZYMOLOGY LA English DT Review ID METAL-CATALYZED OXIDATION; GLUTAMINE-SYNTHETASE; COVALENT MODIFICATION; MECHANISM; RESIDUES; DEGRADATION; RADIOLYSIS; TRYPTOPHAN; CLEAVAGE; TYROSINE RP STADTMAN, ER (reprint author), NHLBI,BIOCHEM LAB,BETHESDA,MD 20892, USA. NR 55 TC 86 Z9 87 U1 2 U2 6 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 258 BP 379 EP 393 PG 15 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08G UT WOS:A1995BE08G00025 PM 8524162 ER PT B AU Ruda, MA AF Ruda, MA BE Gordon, SL Blair, SJ Fine, LJ TI Gene regulation in the dorsal root ganglion in normal and pathologic situations SO REPETITIVE MOTION DISORDERS OF THE UPPER EXTREMITY LA English DT Proceedings Paper CT Workshop on Repetitive Motion Disorders of the Upper Extremity CY JUN 20-22, 1994 CL BETHESDA, MD SP NIAMS, NIOSH, Ctr Dis Control & Prevent, Orthopaed Res & Educ Fdn, Natl Ctr Med Rehab Res, NICHHD, Ctr VDT & Hlth Res, Public Hlth Serv Advisory Comm Employment Persons Disabil C1 NIDR,NEUROBIOL & ANESTHESIOL BRANCH,CELLULAR & MOL MECHANISMS SECT,NATL INST HLTH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ACAD ORTHOPAEDIC SURGEONS PI ROSEMONT PA 6300 NORTH RIVER RD, ROSEMONT, IL 60018 BN 0-89203-143-3 PY 1995 BP 399 EP 406 PG 8 WC Orthopedics SC Orthopedics GA BF35W UT WOS:A1995BF35W00035 ER PT J AU MITCHELL, MD ROMERO, RJ EDWIN, SS TRAUTMAN, MS AF MITCHELL, MD ROMERO, RJ EDWIN, SS TRAUTMAN, MS TI PROSTAGLANDINS AND PARTURITION SO REPRODUCTION FERTILITY AND DEVELOPMENT LA English DT Article; Proceedings Paper CT Thorburn International Symposium in Recognition of the Scientific Contributions to Reproductive and Perinatal Physiology of Geoffrey Donald Thorburn CY AUG 05-09, 1994 CL HAMILTON ISL, AUSTRALIA SP Wyeth Clin Meetings Serv, Monash Univ, Fac Med, Monash Med Ctr, Ctr Mothers & Babies, Beckman Instruments, Austr ID HUMAN-FETAL MEMBRANES; AMNIOTIC-FLUID; G/H SYNTHASE; MESSENGER-RNA; ENDOPEROXIDE SYNTHASE; ARACHIDONIC-ACID; HUMAN-PREGNANCY; UTERINE CERVIX; CDNA CLONING; LABOR AB It seems likely that prostaglandins play a significant part in the mechanisms of parturition both at term and preterm. Concentrations of prostaglandins are increased in the blood, urine and amniotic fluid during labour. There are differences in the concentrations of prostaglandins in amniotic fluid from the forebag and hindbag. Nevertheless, if liquor is sampled only from the hindbag a highly significant increase in prostaglandin concentrations occurs throughout labour. Furthermore, we now have evidence that prostaglandin concentrations in amniotic fluid increase before the onset of labour. Prostaglandins are synthesized by uterine tissues and increased rates of production occur during labour. The amnion, chorion and decidua all contain mRNA for the newly-discovered inducible form of prostaglandin H synthase (PGHS-2) as well as mRNA for the constitutive form (PGHS-1). Using the reverse transcription polymerase chain reaction (RT-PCR) both mRNAs can be detected during late pregnancy whether women are in labour or not. PGHS-2 protein is detected by Western blot analysis in cells derived from all three tissues. There is regulation of PGHS-2 protein amounts by cytokines, phorbol esters and growth factors. For example, in amnion cells interleukin-1 beta induces a rapid increase in PGHS-2 mRNA levels followed by a decrease to undetectable levels within 4 h of treatment; PGHS-2 protein amounts are also elevated by this treatment. Administration of prostaglandins will induce labour and delivery, whereas inhibition of prostaglandin biosynthesis will delay labour and delivery. Hence, increased prostaglandin production is likely to be a key determinant of the onset and progression of the parturient process. C1 NICHHD,PERINATOL BRANCH,BETHESDA,MD 20892. UNIV UTAH,DEPT OBSTET & GYNECOL,SALT LAKE CITY,UT 84132. UNIV UTAH,DEPT PEDIAT,SALT LAKE CITY,UT 84132. RP MITCHELL, MD (reprint author), UNIV AUCKLAND,DEPT PHARMACOL & CLIN PHARMACOL,PRIVATE BAG 92019,AUCKLAND,NEW ZEALAND. RI Mitchell, Murray/A-8639-2010 OI Mitchell, Murray/0000-0002-6167-7176 NR 79 TC 95 Z9 97 U1 0 U2 3 PU C S I R O PUBLICATIONS PI COLLINGWOOD PA 150 OXFORD ST, PO BOX 1139, COLLINGWOOD VICTORIA 3066, AUSTRALIA SN 1031-3613 J9 REPROD FERT DEVELOP JI Reprod. Fertil. Dev. PY 1995 VL 7 IS 3 BP 623 EP 632 DI 10.1071/RD9950623 PG 10 WC Developmental Biology; Reproductive Biology; Zoology SC Developmental Biology; Reproductive Biology; Zoology GA TA833 UT WOS:A1995TA83300039 PM 8606975 ER PT J AU Eddy, EM AF Eddy, EM TI 'Chauvinist genes' of male germ cells: Gene expression during mouse spermatogenesis SO REPRODUCTION FERTILITY AND DEVELOPMENT LA English DT Article; Proceedings Paper CT 7th International Symposium on Spermatology CY OCT 09-14, 1994 CL CAIRNS, AUSTRALIA SP Ansett Austr, Austr Acad Sci, Austr Int Dev Assistance Bur, Austr Soc Reprod Biol, Austr Nat Conservat Agcy, Austr Tourism Commiss, BioRad Labs, Brit Council, William A Cook Pty Ltd, Cryologic Pty Ltd, CSIRO, Div Wildlife & Ecol, Cooperat Res Ctr Biol Vertebrate Pest Control, Fertil Soc Austr, Hamilton Thorn Res, Monash Univ, Inst Reprod & Dev, Inst Engineers, Int Conf Support Scheme, Aust, Qantas Airways, Sense & Vis Electr Syst Ltd, Sheffield, UK, Serono Austr Pty Ltd, Royal Soc, London, Univ Adelaide, Wyeth Austr Pty Ltd ID MESSENGER-RIBONUCLEIC-ACID; LACTATE DEHYDROGENASE-A; NUCLEOTIDE-SEQUENCE; PHOSPHOGLYCERATE KINASE; MOLECULAR-CLONING; HSP70 GENE; CDNA CLONE; DEVELOPMENTAL REGULATION; TYROSINE KINASE; BINDING-SITE RP Eddy, EM (reprint author), NIEHS,REPROD & DEV TOXICOL LAB,GAMETE BIOL SECT,RES TRIANGLE PK,NC 27709, USA. NR 80 TC 33 Z9 38 U1 0 U2 0 PU C S I R O PUBLICATIONS PI COLLINGWOOD PA 150 OXFORD ST, PO BOX 1139, COLLINGWOOD VICTORIA 3066, AUSTRALIA SN 1031-3613 J9 REPROD FERT DEVELOP JI Reprod. Fertil. Dev. PY 1995 VL 7 IS 4 BP 695 EP 704 DI 10.1071/RD9950695 PG 10 WC Developmental Biology; Reproductive Biology; Zoology SC Developmental Biology; Reproductive Biology; Zoology GA TP547 UT WOS:A1995TP54700005 PM 8711206 ER PT B AU FUREYKURKJIAN, ML PIETRINI, P GRAFFRADFORD, N ALEXANDER, GE FREO, U SZCZEPANIK, J SCHAPIRO, MB AF FUREYKURKJIAN, ML PIETRINI, P GRAFFRADFORD, N ALEXANDER, GE FREO, U SZCZEPANIK, J SCHAPIRO, MB BE Iqbal, K Mortimer, JA Winblad, B Wisniewski, HM TI Characterization of neuropsychological function in Alzheimer's disease and patients with prominent visual impairments SO RESEARCH ADVANCES IN ALZHEIMER'S DISEASE AND RELATED DISORDERS LA English DT Proceedings Paper CT 4th International Conference on Alzheimers Disease and Related Disorders CY JUL 29-AUG 03, 1994 CL MINNEAPOLIS, MN RP NIA,NEUROSCI LAB,BLDG 10,BETHESDA,MD 20892, USA. NR 0 TC 2 Z9 2 U1 1 U2 1 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD BN 0-471-95236-2 PY 1995 BP 225 EP 234 PG 10 WC Clinical Neurology SC Neurosciences & Neurology GA BD06G UT WOS:A1995BD06G00025 ER PT J AU GARCIA, RMG ATWATER, I MATHIAS, PCD AF GARCIA, RMG ATWATER, I MATHIAS, PCD TI EFFECTS OF CAFFEINE ON K+ AND CARBAMYLCHOLINE-SECRETORY RESPONSE IN BOVINE CHROMAFFIN CELLS SO RESEARCH COMMUNICATIONS IN ALCOHOL AND SUBSTANCES OF ABUSE LA English DT Article ID INDUCED CATECHOLAMINE SECRETION; ADRENAL-MEDULLARY CELLS; INOSITOL TRISPHOSPHATE; INTRACELLULAR CALCIUM; MUSCARINIC RECEPTOR; ELECTRICAL-ACTIVITY; RELEASE; MOBILIZATION; STIMULATION; ENHANCEMENT AB Catecholamine release was studied in cultured chromaffin cells isolated from bovine adrenal glands by collagenase digestion. Increasing extracellular K+ concentration to 50 mM or addition of the cholinergic agonist, carbamyl choline (Cch), 100 mM, stimulated catecholamine release about 3-fold. Catecholamine release stimulated by K+ and by carbamyl choline (Cch) was dependent on extracellular Ca2+. Caffeine, 50 mM, weakly stimulated catecholamine release in the presence or absence of extracellular Ca2+ Pretreatment with 50 mM K+ in the presence of extracellular Ca2+ enhanced the caffeine-induced release. In the presence of 50 mM caffeine, K+-induced release was inhibited by 70% and Cch-induced release was inhibited by 100%. Caffeine inhibition was dose dependent. K+-induced catecholamine release was concentration dependent, the half-maximal stimulation occurring at about 33 mM K+ which corresponds to a membrane potential of about -30 mV. The voltage dependence of K+-induced release was not shifted by caffeine. Half-maximal stimulation by Cch occurred at about 60 mM; concentration dependent catecholamine release was significantly shifted by 20 mM caffeine. These results suggest that caffeine releases intracellular Ca2+ to induce catecholamine secretion, inhibits of Cch-induced catecholamine release by presumably competition with nicotinic receptors and partial inhibition of K+ responses may be due to interference with the calcium intracellular buffer system, but not with calcium dependent inflow voltage. C1 NIDDK,CELL BIOL & GENET LAB,BETHESDA,MD 20892. RP GARCIA, RMG (reprint author), UNIV ESTADUAL MARINGA,SECRET CELL BIOL LAB,BR-87020900 MARINGA,PARANA,BRAZIL. NR 27 TC 1 Z9 1 U1 0 U2 0 PU P J D PUBLICATIONS LTD PI WESTBURY PA PO BOX 966, WESTBURY, NY 11590 SN 1080-8388 J9 RES COMMUN ALCOHOL S JI Res. Commun. Alcohol Subst. Abus. PY 1995 VL 16 IS 1-2 BP 25 EP 40 PG 16 WC Substance Abuse SC Substance Abuse GA RR563 UT WOS:A1995RR56300002 ER PT J AU BRAUN, CI BENSON, WE REMALEY, NA CHEW, EY FERRIS, FL AF BRAUN, CI BENSON, WE REMALEY, NA CHEW, EY FERRIS, FL TI ACCOMMODATIVE AMPLITUDES IN THE EARLY TREATMENT DIABETIC-RETINOPATHY STUDY - EDTRS REPORT NUMBER-21 SO RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES LA English DT Article DE ACCOMMODATION; DIABETES; PHOTOCOAGULATION; CLINICAL TRIAL AB Purpose: Accommodative amplitude in persons with diabetes was investigated using data collected as part of the Early Treatment Diabetic Retinopathy Study. Methods: Accommodative amplitude was measured at the baseline visit in 1,058 patients who had good visual acuity and who were less than 46 years old. Risk factors for low accommodative amplitude at baseline were evaluated using multivariable linear regression. Change in accommodative amplitude after photocoagulation was evaluated using paired t tests and repeated measures analysis of variance for the 578 patients who underwent followup measurements at the 4-month visit. Results: Accommodative amplitudes in Early Treatment Diabetic Retinopathy Study patients were lower than normal accommodative amplitudes. Older age (P < 0.001) and increased duration of diabetes (P < 0.01) were risk factors associated with low amplitudes of accommodation in the Early Treatment Diabetic Retinopathy Study. Full scatter photocoagulation was associated with an apparently transient additional reduction in accommodative amplitude; a one third diopter loss in accommodative amplitude was demonstrated only at the 4-month visit (P < 0.001). Conclusion: This study demonstrates that diabetes and duration of diabetes, along with age, are important risk factors for reduced accommodative amplitude. These factors along with an apparently transient decrease in accommodative amplitude following scatter photocoagulation should be considered when assessing the accommodative needs of patients with diabetes and when discussing side effects of full scatter photocoagulation. RP BRAUN, CI (reprint author), NEI,DIV BIOMETRY & EPIDEMIOL,BLDG 31,RM 6A-52,31 CTR DR,MSC 2510,BETHESDA,MD 20892, USA. FU Intramural NIH HHS [Z99 EY999999] NR 0 TC 13 Z9 13 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0275-004X J9 RETINA-J RET VIT DIS JI Retin.-J. Retin. Vitr. Dis. PY 1995 VL 15 IS 4 BP 275 EP 281 DI 10.1097/00006982-199515040-00001 PG 7 WC Ophthalmology SC Ophthalmology GA RQ099 UT WOS:A1995RQ09900001 PM 8545570 ER PT J AU GERSHFELD, NL GINSBERG, L AF GERSHFELD, NL GINSBERG, L TI MEMBRANE BILAYER INSTABILITY AS A PATHOGENETIC MECHANISM FOR NEUROLOGICAL DISEASE SO REVIEWS IN THE NEUROSCIENCES LA English DT Article ID PHOSPHOLIPID SURFACE BILAYERS; AIR-WATER-INTERFACE; ALZHEIMERS-DISEASE; METACHROMATIC LEUKODYSTROPHY; MULTIPLE-SCLEROSIS; WHITE MATTER; TEMPERATURE; MYELIN; PERMEABILITY; ERYTHROCYTES C1 NIAMSD,PHYS BIOL LAB,BETHESDA,MD 20892. ROYAL FREE HOSP,SCH MED,DEPT CLIN NEUROSCI,LONDON,ENGLAND. UNIV LONDON,DEPT CLIN NEUROL,LONDON,ENGLAND. RI Ginsberg, Lionel/C-8704-2009 NR 46 TC 10 Z9 10 U1 0 U2 4 PU FREUND PUBLISHING HOUSE PI LONDON PA STE 500, CHESHAM HOUSE, 150 REGENT ST, LONDON, ENGLAND W1R 5FA SN 0334-1763 J9 REV NEUROSCIENCE JI Rev. Neurosci. PD JAN-MAR PY 1995 VL 6 IS 1 BP 1 EP 13 PG 13 WC Neurosciences SC Neurosciences & Neurology GA QX445 UT WOS:A1995QX44500001 PM 7633637 ER PT J AU PASCUAL, L SCHULTHESS, L DEGALPERIN, CZ BORNSTEIN, MH AF PASCUAL, L SCHULTHESS, L DEGALPERIN, CZ BORNSTEIN, MH TI MOTHERS IDEAS ABOUT CHILD TRAINING IN ARGENTINA SO REVISTA INTERAMERICANA DE PSICOLOGIA LA Spanish DT Article ID COMPETENCE AB In this study, we investigated the ideas that Argentine mothers hold about childrearing, comparing mothers of different socioeconomic levels and urban and rural residence. Specifically, we studied three domains of childrearing: social, didactic, and disciplinary. For each domain we analyzed mothers' perceptions of their actual and ideal behaviors and of their husbands' actual and ideal behaviors, and mothers' satisfaction with their own and their husbands' behaviors. The results showed significant differences between mothers' ideas about their own and their husbands' behavior, especially in the social and didactic domains. Socioeconomic level and place of residence had limited effects on mothers' ideas about parenting. C1 UNIV BUENOS AIRES,BUENOS AIRES,DF,ARGENTINA. CTR ASISTENCIA VICTIMA DELITO,CORDOBA,ARGENTINA. RP PASCUAL, L (reprint author), NICHHD,BLDG 31,ROOM B2B15,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 30 TC 3 Z9 3 U1 0 U2 0 PU INTERAMER SOC PSYCHOL UNIV CALIFORNIA PI LOS ANGELES PA SPANISH SPEAKING MENTAL HEALTH RES CENTER, LOS ANGELES, CA 90024 SN 0034-9690 J9 REV INTERAM PSICOL JI Rev. Interam. Psicol. PY 1995 VL 29 IS 1 BP 23 EP 38 PG 16 WC Psychology SC Psychology GA TG127 UT WOS:A1995TG12700003 ER PT J AU BENNETT, P AF BENNETT, P TI CIGARETTE-SMOKING AND ALCOHOL, INDEPENDENT RISK-FACTORS FOR NON-INSULIN-DEPENDENT DIABETES-MELLITUS SO REVUE D EPIDEMIOLOGIE ET DE SANTE PUBLIQUE LA French DT Editorial Material RP BENNETT, P (reprint author), NIDDK,1550 E INDIAN SCH RD,PHOENIX,AZ 85014, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MASSON EDITEUR PI PARIS 06 PA 120 BLVD SAINT-GERMAIN, 75280 PARIS 06, FRANCE SN 0398-7620 J9 REV EPIDEMIOL SANTE JI Rev. Epidemiol. Sante Publique PY 1995 VL 43 IS 5 BP 511 EP 513 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TC405 UT WOS:A1995TC40500015 ER PT B AU Fitzmaurice, JM AF Fitzmaurice, JM BE Grundfest, WS TI Developing and sharing medical effectiveness information SO ROLE OF TECHNOLOGY IN THE COST OF HEALTH CARE: PROVIDING THE SOLUTIONS: HEALTH CARE TECHNOLOGY POLICY II SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Health Care Technology Policy II - the Role of Technology in the Cost of Health Care: Providing the Solutions CY MAY 10-12, 1995 CL ARLINGTON, VA SP Soc Photo Opt Instrumentat Engineers, Whitaker Fdn, NIST, Adv Technol Programs, Cedars Sinai Medallions Charitable Fund, Hamamatsu Corp, Mayo Clin, IEEE, Engn Med & Biol Soc C1 AGCY HLTH CARE POLICY & RES,CTR INFORMAT TECHNOL,DEPT HLTH & HUMAN SERV,ROCKVILLE,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1856-0 J9 P SOC PHOTO-OPT INS PY 1995 VL 2499 BP 34 EP 43 PG 10 WC Computer Science, Information Systems; Optics SC Computer Science; Optics GA BE37N UT WOS:A1995BE37N00005 ER PT B AU Shtern, F AF Shtern, F BE Grundfest, WS TI Bridging the gap between research and marketing: Private & public partnerships SO ROLE OF TECHNOLOGY IN THE COST OF HEALTH CARE: PROVIDING THE SOLUTIONS: HEALTH CARE TECHNOLOGY POLICY II SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Health Care Technology Policy II - the Role of Technology in the Cost of Health Care: Providing the Solutions CY MAY 10-12, 1995 CL ARLINGTON, VA SP Soc Photo Opt Instrumentat Engineers, Whitaker Fdn, NIST, Adv Technol Programs, Cedars Sinai Medallions Charitable Fund, Hamamatsu Corp, Mayo Clin, IEEE, Engn Med & Biol Soc C1 NCI,DIAGNOST IMAGING RES BRANCH,BETHESDA,MD 20852. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1856-0 J9 P SOC PHOTO-OPT INS PY 1995 VL 2499 BP 255 EP 258 PG 4 WC Computer Science, Information Systems; Optics SC Computer Science; Optics GA BE37N UT WOS:A1995BE37N00029 ER PT B AU Colby, CL Duhamel, JR AF Colby, CL Duhamel, JR BE King, J Pribram, KH TI Heterogeneity of extrastriate visual areas and multiple parietal areas in the macaque monkey SO SCALE IN CONSCIOUS EXPERIENCE: IS THE BRAIN TOO IMPORTANT TO BE LEFT TO SPECIALISTS TO STUDY? LA English DT Proceedings Paper CT 3rd Appalachian Conference on Behavioral Neurodynamics CY SEP 22-25, 1995 CL RADFORD, VA SP Int Neural Network Soc C1 NEI,SENSORIMOTOR RES LAB,NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC PUBL PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430 BN 0-8058-2178-3 PY 1995 BP 65 EP & PG 22 WC Computer Science, Artificial Intelligence; Neurosciences; Physics, Applied; Psychology, Experimental SC Computer Science; Neurosciences & Neurology; Physics; Psychology GA BE62C UT WOS:A1995BE62C00005 ER PT B AU Mishkin, M AF Mishkin, M BE King, J Pribram, KH TI Cerebral memory circuits SO SCALE IN CONSCIOUS EXPERIENCE: IS THE BRAIN TOO IMPORTANT TO BE LEFT TO SPECIALISTS TO STUDY? LA English DT Proceedings Paper CT 3rd Appalachian Conference on Behavioral Neurodynamics CY SEP 22-25, 1995 CL RADFORD, VA SP Int Neural Network Soc C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC PUBL PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430 BN 0-8058-2178-3 PY 1995 BP 89 EP & PG 14 WC Computer Science, Artificial Intelligence; Neurosciences; Physics, Applied; Psychology, Experimental SC Computer Science; Neurosciences & Neurology; Physics; Psychology GA BE62C UT WOS:A1995BE62C00006 ER PT B AU Miller, EK AF Miller, EK BE King, J Pribram, KH TI Neocortical mechanisms for visual memory SO SCALE IN CONSCIOUS EXPERIENCE: IS THE BRAIN TOO IMPORTANT TO BE LEFT TO SPECIALISTS TO STUDY? LA English DT Proceedings Paper CT 3rd Appalachian Conference on Behavioral Neurodynamics CY SEP 22-25, 1995 CL RADFORD, VA SP Int Neural Network Soc C1 NIMH,NEUROPSYCHOL LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC PUBL PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430 BN 0-8058-2178-3 PY 1995 BP 105 EP & PG 10 WC Computer Science, Artificial Intelligence; Neurosciences; Physics, Applied; Psychology, Experimental SC Computer Science; Neurosciences & Neurology; Physics; Psychology GA BE62C UT WOS:A1995BE62C00007 ER PT J AU Lerche, A Rasmussen, N Wandall, JH Bohr, VA AF Lerche, A Rasmussen, N Wandall, JH Bohr, VA TI Staphylococcus aureus meningitis: A review of 28 consecutive community-acquired cases SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID BACTERIAL-MENINGITIS AB From 1966 to 1989 a total of 1,830 cases of bacterial meningitis were recorded at the Department of Infectious Diseases, Rigshospitalet, Denmark, Staphylococcus aureus meningitis accounted for 44 (2.4%) of these cases. Among these, 28 cases were classified as community-acquired S. aureus meningitis. The mortality rate for these cases was 43%. A retrospective study of clinical features and parameters in these community-acquired cases showed the following conditions to be associated with a high mortality risk: advanced age, an underlying condition requiring artificial ventilation, cardiovascular disease and immune deficiencies, At admission, more than 75% of the patients had fever, nuchal rigidity and decreased consciousness. In 57% of cases the focus for the S. aureus infection was endocarditis, pneumonia or skin infections. All the patients had complications due to the meningitis, the major one being insufficient respiration. Autopsy performed in 9 of the 12 fatalities showed endocarditis in 5, pneumonia in 4, and pyelonephritis in 2, All of the brains examined at autopsy showed cerebral and subarachnoid hemorrhage. C1 RIGSHOSP,DEPT INFECT DIS,DK-2100 COPENHAGEN,DENMARK. RIGSHOSP,DEPT OTOLARYNGOL HEAD & NECK SURG,DK-2100 COPENHAGEN,DENMARK. NIH,NIA,GENET MOLEC LAB,BALTIMORE,MD. RP Lerche, A (reprint author), FREDERIKSBERG UNIV HOSP,DEPT MED B,INFECT DIS UNIT,DK-2000 COPENHAGEN F,DENMARK. NR 16 TC 23 Z9 23 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1995 VL 27 IS 6 BP 569 EP 573 DI 10.3109/00365549509047069 PG 5 WC Infectious Diseases SC Infectious Diseases GA TZ304 UT WOS:A1995TZ30400006 PM 8685635 ER PT J AU Speir, E Huang, ES Modali, R Leon, MB Shawl, F Finkel, T Epstein, SE AF Speir, E Huang, ES Modali, R Leon, MB Shawl, F Finkel, T Epstein, SE TI Interaction of human cytomegalovirus with p53: Possible role in coronary restenosis SO SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 5th International Cytomegalovirus Conference CY MAY 21-24, 1995 CL STOCKHOLM, SWEDEN ID CELLULAR TUMOR-ANTIGEN; SV40-TRANSFORMED CELLS; TRANSFORMED-CELLS; PROTEIN; CARCINOMA; CANCER; EXPRESSION; MUTATIONS AB Restenosis occurs in 25-50% of patients, Within 1-6 months after coronary angioplasty, excessive injury-induced smooth muscle cell (SMC) proliferation contributes to the development of restenosis; its causes remain unknown, The results of this study implicate human cytomegalovirus (HCMV) and HCMV-induced abnormalities in p53 function in the restenosis process. Almost 40% of restenosis lesions, obtained by atherectomy, demonstrated increased SMC p53 levels by p53 immunopositivity; sequencing revealed the p53 to be the wild type, A strong correlation was found between p53 immunopositivity and the presence of HCMV DNA, Moreover, the HCMV IE84 protein co-immunoprecipitates with p53, and p53 transcriptional capacity is reduced by IE84, Thus, HCMV may play a causal role in restenosis, which may be at least partly mediated by inhibiting p53 suppressor effects. C1 UNIV N CAROLINA,LINEBERGER CANC RES CTR,MED CTR,CHAPEL HILL,NC 27599. BIOSERVE BIOTECHNOL LTD,LAUREL,MD. WASHINGTON HOSP CTR,WASHINGTON CARDIOL CTR,WASHINGTON,DC 20010. WASHINGTON ADVENTIST HOSP,TAKOMA PK,MD. RP Speir, E (reprint author), NHLBI,NIH,CARDIOL BRANCH,BLDG 10,BETHESDA,MD 20892, USA. NR 21 TC 0 Z9 0 U1 0 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5548 J9 SCAND J INFECT DIS JI Scand. J. Infect. Dis. PY 1995 SU 99 BP 78 EP 81 PG 4 WC Infectious Diseases SC Infectious Diseases GA TZ687 UT WOS:A1995TZ68700016 ER PT J AU IVERSEN, P MADSEN, PO CORLE, DK AF IVERSEN, P MADSEN, PO CORLE, DK TI RADICAL PROSTATECTOMY VERSUS EXPECTANT TREATMENT FOR EARLY CARCINOMA OF THE PROSTATE - 23-YEAR FOLLOW-UP OF A PROSPECTIVE RANDOMIZED STUDY SO SCANDINAVIAN JOURNAL OF UROLOGY AND NEPHROLOGY LA English DT Article DE RADICAL PROSTATECTOMY; EXPECTANT TREATMENT; EARLY CARCINOMA OF THE PROSTATE ID STAGE-I; CANCER; MANAGEMENT; SURVIVAL; IMPACT; RISK AB In a study by the Veterans Administration Cooperative Urological Research Group (VACURG),142 patients with localized prostate cancer, VACURG stage I and II, were randomized between radical prostatectomy plus placebo versus placebo alone as initial treatment. 111 patients were evaluable for treatment comparison. Median follow-up for survival is 23 years. The prognostic value of Gleason histologic grading was confirmed. A difference in overall survival in favor of radical prostatectomy was observed in stage I patients. However, after adjustment for imbalance in age distribution, no statistically significant differences in survival could be demonstrated in either stage or in both stages combined. The results are discussed considering the small sample size and the limited statistical power of the study. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,UROL SECT,MADISON,WI 53705. UNIV COPENHAGEN,RIGSHOSP,DEPT UROL,DK-2100 COPENHAGEN,DENMARK. UNIV WISCONSIN,SCH MED,DEPT SURG,MADISON,WI. NCI,DIV CANC CONTROL,BETHESDA,MD 20892. NR 34 TC 0 Z9 0 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5599 J9 SCAND J UROL NEPHROL JI Scand. J. Urol. Nephrol. PY 1995 SU 172 BP 65 EP 72 PG 8 WC Urology & Nephrology SC Urology & Nephrology GA RX700 UT WOS:A1995RX70000013 ER PT J AU Daniel, LN Mao, Y Williams, AO Saffiotti, U AF Daniel, LN Mao, Y Williams, AO Saffiotti, U TI Direct interaction between crystalline silica and DNA - A proposed model for silica carcinogenesis SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH LA English DT Article; Proceedings Paper CT 2nd International Symposium on Silica, Silicosis, and Cancer CY OCT 27-30, 1993 CL SAN FRANCISCO, CA DE carcinogenesis mechanisms; deoxyribonucleic acid; DNA binding; FRLE cell line; electron microscopy; infrared spectra; quartz ID MAMMALIAN-CELLS; INFRARED-SPECTROSCOPY; QUARTZ; DUST; INDUCTION; BINDING; TUMORS; DAMAGE; FIBER; RATS AB Crystalline silica in aqueous buffer produced oxygen radicals that mediated in vitro DNA (deoxyribonucleic acid) strand breakage. The oxidized DNA base, thymine glycol, was also produced. The hydroxyl radical, responsible for most DNA damage, has a reaction distance of about 15 Angstroms, requiring close contact of silica with DNA. Fourier transform infrared spectroscopy of incubations of quartz particles with DNA showed distinct alterations in both DNA and quartz spectra and therefore indicated extensive hydrogen bonding between surface silanol groups and the phosphate-sugar backbone of DNA. Electron microscopy and energy dispersive X-ray spectroscopy of alveolar epithelial cells in fetal rat lung, exposed to quartz in culture, showed localization of quartz particles in the nuclei and mitotic spindles. Direct interaction of crystalline silica with DNA may be important in silica carcinogenesis by anchoring DNA close to sites of free radical production on the silica surface, or by interfering with DNA replication, repair, or the mitotic process. C1 NCI, LAB EXPTL PATHOL, BETHESDA, MD 20892 USA. NR 37 TC 37 Z9 40 U1 0 U2 1 PU SCANDINAVIAN JOURNAL WORK ENVIRONMENT & HEALTH PI HELSINKI PA TOPELIUKSENKATU 41A, SF-00250 HELSINKI, FINLAND SN 0355-3140 J9 SCAND J WORK ENV HEA JI Scand. J. Work Environ. Health PY 1995 VL 21 SU 2 BP 22 EP 26 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TV359 UT WOS:A1995TV35900006 PM 8929683 ER PT J AU Williams, AO Saffiotti, U AF Williams, AO Saffiotti, U TI Transforming growth factor beta 1, ras and p53 in silica-induced fibrogenesis and carcinogenesis SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH LA English DT Article; Proceedings Paper CT 2nd International Symposium on Silica, Silicosis, and Cancer CY OCT 27-30, 1993 CL SAN FRANCISCO, CA DE alveolar type II cells; immunohistochemistry; p53; p21 ras; rat; TGF-beta 1 ID IMMUNOHISTOCHEMICAL LOCALIZATION; COLLAGEN-SYNTHESIS; EPITHELIAL-CELLS; FIBROSIS; RATS AB The pathogenesis of mesenchymal and epithelial lung reactions was studied after a single intratracheal instillation of quartz into rats. Relationships between transforming growth factor beta 1 (TGF-beta 1) and the ras and p53 genes were investigated in silicosis and associated lung cancer. Immunohistochemical reactivity to mature TGF-beta 1 was localized intracellularly in fibroblasts and macrophages at the periphery of silicotic granulomas and in stroma adjacent to hyperplastic alveolar type II cells and extracellularly in connective tissue matrix adjacent to hyperplastic alveolar type II cells. TGF-beta 1 precursor was localized intracellularly in hyperplastic alveolar type II cells adjacent to granulomas and in the cells of adenomas, but not in carcinomas. Hematite-treated controls showed no reactivity to TGF-beta 1. Immunohistochemical localization of pan-reactive p21 ras protein in quartz-treated rat lunes was increased in hyperplastic alveolar type II cells adjacent to granulomas, but not in adenomas and carcinomas. Foci of nuclear immunoreactivity to p53 protein were observed in 25% of the carcinomas. C1 NCI, LAB EXPTL PATHOL, BETHESDA, MD 20892 USA. NR 17 TC 16 Z9 16 U1 0 U2 1 PU SCANDINAVIAN JOURNAL WORK ENVIRONMENT & HEALTH PI HELSINKI PA TOPELIUKSENKATU 41A, SF-00250 HELSINKI, FINLAND SN 0355-3140 J9 SCAND J WORK ENV HEA JI Scand. J. Work Environ. Health PY 1995 VL 21 SU 2 BP 30 EP 34 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TV359 UT WOS:A1995TV35900008 PM 8929685 ER PT J AU Dosemeci, M McLaughlin, JK Chen, JQ Hearl, F Chen, RG McCawley, M Wu, Z Peng, KL Chen, AL Rexing, SH Blot, WJ AF Dosemeci, M McLaughlin, JK Chen, JQ Hearl, F Chen, RG McCawley, M Wu, Z Peng, KL Chen, AL Rexing, SH Blot, WJ TI Historical total and respirable silica dust exposure levels in mines and pottery factories in China SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH LA English DT Article; Proceedings Paper CT 2nd International Symposium on Silica, Silicosis, and Cancer CY OCT 27-30, 1993 CL SAN FRANCISCO, CA DE Chinese mines and potteries; exposure assessment; industrial hygiene measurements; occupational exposure; retrospective assessment; silica ID WORKERS AB Historical exposure estimates of total dust and respirable silica were made in a recent nested case-referent study of lung cancer among mine and pottery workers in China. Exposure to total dust and respirable silica was assessed in 20 mines and 9 pottery factories. The average total dust concentration was 7.26 mg . m(-3) with a range from 17.68 mg . m(-3) in the 1950s to 3.85 mg . m(-3) in the 1980s, while the average respirable silica dust was 1.22 mg . m(-3), with a range from 3.89 mg . m(-3) in the 1950s to 0.43 mg . m(-3) in the 1980s. The highest respirable silica dust occurred in the underground mining operations (1.43 mg . m(-3)), particularly for manual drillers (9.03 mg . m(-3)). Among all facility types, tungsten mines had the highest respirable silica dust exposure (1.75 mg . m(-3)), while the lowest exposure occurred in copper-iron mines (0.32 mg . m(-3)). C1 NCI,EPIDEMIOL & BIOSTAT PROGRAM,BETHESDA,MD 20892. TONGJI MED UNIV,DEPT LABOR HLTH & OCCUPAT DIS,WUHAN,PEOPLES R CHINA. NIOSH,MORGANTOWN,WV. WESTAT CORP,ROCKVILLE,MD. NR 16 TC 17 Z9 18 U1 1 U2 2 PU SCAND J WORK ENV HEALTH PI HELSINKI PA TOPELIUKSENKATU 41A, SF-00250 HELSINKI, FINLAND SN 0355-3140 J9 SCAND J WORK ENV HEA JI Scand. J. Work Environ. Health PY 1995 VL 21 SU 2 BP 39 EP 43 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TV359 UT WOS:A1995TV35900010 PM 8929687 ER PT J AU Amandus, HE Shy, C Castellan, RM Blair, A Heineman, EF AF Amandus, HE Shy, C Castellan, RM Blair, A Heineman, EF TI Silicosis and lung cancer among workers in North Carolina dusty trades SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH LA English DT Article; Proceedings Paper CT 2nd International Symposium on Silica, Silicosis, and Cancer CY OCT 27-30, 1993 CL SAN FRANCISCO, CA DE dusty trades; lung cancer; silicosis AB In 1940-1983, 760 cases of silicosis were identified among male North Carolina (NC) workers in dusty trades. Vital status was ascertained through 1983 for 714 silicotics, and death certificates were obtained for 546 of the 550 decedents. The standardized mortality ratio (SMR) for lung cancer based on United Stares rates was 2.6 [95% confidence interval (95% CI) 1.8-3.6] for whites, 2.3 (95% CI 1.5-3.4) for whites unexposed to other known occupational carcinogens, and 2.4 (95% CI 1.5-3.6) for whites with no other exposure and diagnosed with silicosis while still employed in dusty trades. In addition, the age- and smoking-adjusted rate for silicotics was 3.9 times higher (95% CI 2.4-6.4) than that of nonsilicotic metal miners. This analysis effectively controlled for confounding by age, cigarette smoking, exposure to other occupational carcinogens, and detection bias. The results congrue with the hypothesis of an association between silicosis and lung cancer. C1 UNIV N CAROLINA,DEPT EPIDEMIOL,CHAPEL HILL,NC 27515. NCI,OCCUPAT STUDIES SECT,ROCKVILLE,MD. RP Amandus, HE (reprint author), NIOSH,944 CHESTNUT RIDGE RD,MAILSTOP P 1133,MORGANTOWN,WV 26505, USA. NR 8 TC 15 Z9 15 U1 2 U2 2 PU SCAND J WORK ENV HEALTH PI HELSINKI PA TOPELIUKSENKATU 41A, SF-00250 HELSINKI, FINLAND SN 0355-3140 J9 SCAND J WORK ENV HEA JI Scand. J. Work Environ. Health PY 1995 VL 21 SU 2 BP 81 EP 83 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TV359 UT WOS:A1995TV35900021 PM 8929698 ER PT J AU Goldsmith, DF Wagner, GR Saffiotti, U Rabovsky, J Leigh, J AF Goldsmith, DF Wagner, GR Saffiotti, U Rabovsky, J Leigh, J TI Future research needs in the silica, silicosis and cancer field SO SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH LA English DT Article DE biology, cancer; future research; physicochemistry; prevention; silica; silicosis; risk assessment ID AFRICAN-GOLD MINERS; LUNG-CANCER; MORTALITY; INDUCTION; EXPOSURE; QUARTZ C1 NIOSH, MORGANTOWN, WV USA. NCI, LAB EXPTL PATHOL, BETHESDA, MD 20892 USA. CALIF ENVIRONM PROTECT AGCY, OFF ENVIRONM HLTH HAZARD ASSESSMENT, BERKELEY, CA USA. NIOSH, WORKSAFE AUSTRALIA, SYDNEY, NSW, AUSTRALIA. RP Goldsmith, DF (reprint author), WESTERN CONSORTIUM PUBL HLTH, 2001 ADDISON ST, SUITE 200, BERKELEY, CA 94704 USA. NR 30 TC 7 Z9 7 U1 0 U2 1 PU SCANDINAVIAN JOURNAL WORK ENVIRONMENT & HEALTH PI HELSINKI PA TOPELIUKSENKATU 41A, SF-00250 HELSINKI, FINLAND SN 0355-3140 J9 SCAND J WORK ENV HEA JI Scand. J. Work Environ. Health PY 1995 VL 21 SU 2 BP 115 EP 117 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TV359 UT WOS:A1995TV35900030 PM 8929707 ER PT J AU MCCUTCHEN, CW AF MCCUTCHEN, CW TI TRANSMISSION-LINE PROBES FOR SCANNING PHOTON-TUNNELING MICROSCOPY SO SCANNING LA English DT Article DE SCANNING; PHOTON-TUNNELING; TRANSMISSION LINE AB Because metalized light probes for scanning photon-tunneling microscopy are tapered waveguides beyond cut-off tapered coaxial transmission lines have been tried instead. It is shown here that insertion losses in such a line can be reduced by asymmetrically phase-apodizing the illuminating lens. Alternatively, a strip rather than a coaxial line can be used. RP MCCUTCHEN, CW (reprint author), NIDDKD,CELL BIOL & GENET LAB,BETHESDA,MD 20892, USA. NR 4 TC 5 Z9 5 U1 0 U2 2 PU FAMS INC PI MAHWAH PA BOX 832, MAHWAH, NJ 07430 SN 0161-0457 J9 SCANNING JI Scanning PD JAN-FEB PY 1995 VL 17 IS 1 BP 15 EP 17 PG 3 WC Instruments & Instrumentation; Microscopy SC Instruments & Instrumentation; Microscopy GA QH234 UT WOS:A1995QH23400003 PM 7704316 ER PT S AU LILLRANK, SM WEINBERGER, DR AF LILLRANK, SM WEINBERGER, DR BE Fog, R Gerlach, J Hemmingsen, R TI NEUROIMAGING STUDIES OF SCHIZOPHRENIA - IMPLICATIONS FOR A NEURODEVELOPMENTAL MODEL SO SCHIZOPHRENIA: AN INTEGRATED VIEW SE ALFRED BENZON SYMPOSIUM SERIES LA English DT Proceedings Paper CT Alfred Benzon Symposium on Schizophrenia - An Integrated View CY JUN 05-09, 1994 CL COPENHAGEN, DENMARK SP ALFRED BENZON FDN C1 NIMH,NEUROSCI CTR ST ELIZABETHS,CLIN BRAIN DISORDERS BRANCH,WASHINGTON,DC 20032. NR 0 TC 2 Z9 2 U1 0 U2 0 PU MUNKSGAARD PI COPENHAGEN K PA 35 NORRE SOGADE POSTBOX 2148, DK-1016 COPENHAGEN K, DENMARK SN 0105-3639 BN 87-16-11487-6 J9 ALFRED BENZON SYMP S PY 1995 VL 38 BP 207 EP 216 PG 10 WC Psychiatry SC Psychiatry GA BC73Q UT WOS:A1995BC73Q00014 ER PT J AU TORREY, EF YOLKEN, RH AF TORREY, EF YOLKEN, RH TI COULD SCHIZOPHRENIA BE A VIRAL ZOONOSIS TRANSMITTED FROM HOUSE CATS SO SCHIZOPHRENIA BULLETIN LA English DT Editorial Material ID PRENATAL EXPOSURE; INFLUENZA EPIDEMICS; ADULT SCHIZOPHRENIA; INFECTION; DISORDER; SEASON; BIRTH AB Studies have suggested that some cases of schizophrenia may be caused by viruses. We hypothesize that such cases may be cases of viral zoonosis transmitted primarily from house cats. Epidemiological aspects of schizophrenia and a case-control questionnaire support this hypothesis. C1 JOHNS HOPKINS UNIV,SCH MED,NEUROVIROL LAB,BALTIMORE,MD. RP TORREY, EF (reprint author), ST ELIZABETH HOSP,NIMH,CTR NEUROSCI,TWIN STUDY UNIT,WASHINGTON,DC 20032, USA. NR 34 TC 53 Z9 53 U1 1 U2 6 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 167 EP 171 PG 5 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200001 PM 7631163 ER PT J AU MIRSKY, AF AF MIRSKY, AF TI ISRAELI HIGH-RISK STUDY - EDITORS INTRODUCTION SO SCHIZOPHRENIA BULLETIN LA English DT Article ID SCHIZOPHRENIA; CHILDREN AB The National Institute of Mental Health joint study by the United States and Israel, known as the Israeli High-Risk Study, is a unique long-term followup investigation of children at genetic risk for schizophrenia. We compared the development of psychiatric disorder in two groups of such children, one group raised in kibbutz environments, the other by their own parents. Matched controls were studied as well. The subjects were evaluated at ages 11, 17, and 26; an extensive battery of cognitive and clinical tests, as well as psychophysiological and diagnostic procedures, was used. This issue of the Schizophrenia Bulletin reports and summarizes evaluations conducted when the subjects were in their early thirties, as well as some previously unreported data obtained when the subjects were 17 years old. RP MIRSKY, AF (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C-110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 29 TC 5 Z9 5 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 179 EP 182 PG 4 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200003 PM 7631165 ER PT J AU INGRAHAM, LJ KUGELMASS, S FRENKEL, E NATHAN, M MIRSKY, AF AF INGRAHAM, LJ KUGELMASS, S FRENKEL, E NATHAN, M MIRSKY, AF TI TWENTY-5-YEAR FOLLOW-UP OF THE ISRAELI HIGH-RISK STUDY - CURRENT AND LIFETIME PSYCHOPATHOLOGY SO SCHIZOPHRENIA BULLETIN LA English DT Article ID SCHIZOTYPAL PERSONALITY-TRAITS; DISORDER AB Current and lifetime psychopathology was assessed in 50 Israeli children of parents with schizophrenia who were either of kibbutz families and raised collectively with the help of child care workers, or of urban families and raised by their parents. Index subjects were compared with 50 matched control children of healthy parents by means of the Schedule for Affective Disorders and Schizophrenia-Israel. Subjects were evaluated in adulthood at a mean age of 31 years; schizophrenia was found exclusively among children of ill parents, and no effect of town or kibbutz rearing on risk for schizophrenia was observed. Major affective illness was more common among kibbutz index subjects. Affective symptomatology observed in some index parents was evenly distributed among town and kibbutz parents and was not related to the diagnosis of affective disorders in at-risk children. Current adult functioning was similar between town- and kibbutz-raised subjects (and in general reflected good adjustment); an excess of personality disorders was found among index subjects. The present findings support the concept that both familial and environmental factors operate in the expression of psychopathology. C1 HEBREW UNIV JERUSALEM,DEPT PSYCHOL,IL-91905 JERUSALEM,ISRAEL. UNIV HAIFA,INST KIBBUTZ EDUC,IL-31999 HAIFA,ISRAEL. RP INGRAHAM, LJ (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C-110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 20 TC 39 Z9 39 U1 0 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 183 EP 192 PG 10 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200004 PM 7631166 ER PT J AU MIRSKY, AF INGRAHAM, LJ KUGELMASS, S AF MIRSKY, AF INGRAHAM, LJ KUGELMASS, S TI NEUROPSYCHOLOGICAL ASSESSMENT OF ATTENTION AND ITS PATHOLOGY IN THE ISRAELI COHORT SO SCHIZOPHRENIA BULLETIN LA English DT Article ID HIGH-RISK CHILDREN; SCHIZOPHRENIA; PERFORMANCE; ABNORMALITIES; RESPONSES; BRAIN AB We assessed attention in 63 of the 98 traceable living subjects of the original 100 in the National Institute of Mental Health (NIMH) joint study of schizophrenia by the United States and Israel, known as the Israeli High-Risk Study cohort; their mean age was 32 years. These data were supplemented, for comparative purposes, with those obtained on 31 normal control and 17 schizophrenia subjects studied at NIMH. The results suggest that attention skills of the adult children of a parent with schizophrenia fall between those of schizophrenia patients and controls, and that measures of sustained attention and the ability to focus and execute provide the best discrimination among groups. Post hoc analyses revealed that poor scores on simple tests of attention obtained in childhood were associated with the development of disorders in adulthood. Low scores on a digit cancellation test at age 11, but not at age 17, predicted which of the children at genetic risk would develop schizophrenia spectrum disorders diagnosed at ages 26 and 32. C1 HEBREW UNIV JERUSALEM,JERUSALEM,ISRAEL. RP MIRSKY, AF (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C-110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 39 TC 34 Z9 35 U1 2 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 193 EP 204 PG 12 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200005 PM 7631167 ER PT J AU KUGELMASS, S FABER, N INGRAHAM, LJ FRENKEL, E NATHAN, M MIRSKY, AF BENSHAKHAR, G AF KUGELMASS, S FABER, N INGRAHAM, LJ FRENKEL, E NATHAN, M MIRSKY, AF BENSHAKHAR, G TI REANALYSIS OF SCOR AND ANXIETY MEASURES IN THE ISRAELI HIGH-RISK STUDY SO SCHIZOPHRENIA BULLETIN LA English DT Article ID CONDUCTANCE ORIENTING RESPONSE; NERVOUS-SYSTEM ACTIVITY; SKIN-CONDUCTANCE; ELECTRODERMAL ACTIVITY; SCHIZOPHRENIA; CHILDREN; DEPRESSION; PSYCHOPHYSIOLOGY; DISORDERS AB In an earlier study, skin conductance orienting response (SCOR) and anxiety measures obtained when the subjects of the Israeli High-Risk Study were 11 years old were analyzed, using adult diagnostic information, when the subjects were 26 years old. The present study considers similar data obtained from most of this sample when the subjects were 16 years old. As in the earlier analysis, those subjects who would receive a schizophrenia spectrum diagnosis at 26 had higher anxiety ratings at age 16. Nondiagnosed index subjects also had significantly higher anxiety ratings than the nondiagnosed controls. The subjects who would receive affective spectrum diagnoses at age 26 had the most hyporesponsive SCORs, as predicted, while the subjects who would later be diagnosed in the schizophrenia spectrum had an unexpected hyperresponsive SCOR to the dishabituation tone in a habituation series. Further consideration of the long-term stability of SCORs seems necessary; they may be related to the developing psychopathological processes. C1 NIMH,PSYCHOL & PSYCHOPATHOL LAB,BETHESDA,MD 20892. HEBREW UNIV JERUSALEM,DEPT PSYCHOL,IL-91905 JERUSALEM,ISRAEL. UNIV HAIFA,INST KIBBUTZ EDUC,IL-31999 HAIFA,ISRAEL. HEBREW UNIV JERUSALEM,FAC SOCIAL SCI,JERUSALEM,ISRAEL. NR 44 TC 15 Z9 15 U1 0 U2 2 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 205 EP 217 PG 13 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200006 PM 7631168 ER PT J AU FRENKEL, E KUGELMASS, S NATHAN, M INGRAHAM, LJ AF FRENKEL, E KUGELMASS, S NATHAN, M INGRAHAM, LJ TI LOCUS OF CONTROL AND MENTAL-HEALTH IN ADOLESCENCE AND ADULTHOOD SO SCHIZOPHRENIA BULLETIN LA English DT Article ID INTERNAL-EXTERNAL-CONTROL; HIGH-RISK AB Eighty-nine subjects of the original sample of the National Institute of Mental Health joint study by the United States and Israel, known as the Israeli High-Risk Study, were given a clinical interview and a questionnaire measuring locus of control (LOC) during the second phase of the study, when the subjects were adolescents. During phases 3 and 4, approximately 8 and 15 years later, the subjects were psychiatrically assessed and 56 of them repeated the LOC questionnaire. The two measures of LOC were correlated, as were general assessments of mental health (MH). Adolescent LOC was related to lifetime MH, although LOC and MH were not related to each other concurrently in either adolescence or adulthood. The best predictive model for lifetime MH outcomes was a combination of adolescent MH and LOC variables; background variables, including parental schizophrenia, were superfluous. The data suggest that whereas adolescent MH is the best predictor of general MH, adolescent LOC is the better predictor of schizophrenia and major affective disorders. C1 NIMH,PSYCHOL & PSYCHOPATHOL LAB,BETHESDA,MD 20892. HEBREW UNIV JERUSALEM,DEPT PSYCHOL,IL-91905 JERUSALEM,ISRAEL. UNIV HAIFA,INST KIBBUTZ EDUC,IL-31999 HAIFA,ISRAEL. NR 23 TC 33 Z9 33 U1 0 U2 5 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 219 EP 226 PG 8 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200007 PM 7631169 ER PT J AU MIRSKY, AF KUGELMASS, S INGRAHAM, LJ FRENKEL, E NATHAN, M AF MIRSKY, AF KUGELMASS, S INGRAHAM, LJ FRENKEL, E NATHAN, M TI OVERVIEW AND SUMMARY - 25-YEAR FOLLOW-UP OF HIGH-RISK CHILDREN SO SCHIZOPHRENIA BULLETIN LA English DT Article ID JERUSALEM INFANT DEVELOPMENT; ISRAELI HIGH-RISK; AFFECTIVE-DISORDERS; SCHIZOPHRENIA; PERFORMANCE; INTERVIEW; ATTENTION; VULNERABILITY; DEVIANCE; OUTCOMES AB We report a 25-year followup of a group of 50 children at genetic risk for schizophrenia (by virtue of having a parent with the disorder) and 50 matched controls. The children who eventually developed schizophrenia spectrum disorders, including schizophrenia, were identifiable by cognitive-psychophysiological, neurointegrative, and social/personality traits in the preteenage period. The children at risk were also more likely to develop other Axis I disorders, chiefly affective. Moreover, the risk of Axis I disorders was significantly greater among children raised in the group atmosphere of a kibbutz than among those raised in their own nuclear families in cities and towns in Israel. The study is a unique contribution to knowledge of factors underlying the development of psychopathology. C1 HEBREW UNIV JERUSALEM,DEPT PSYCHOL,IL-91905 JERUSALEM,ISRAEL. UNIV HAIFA,INST KIBBUTZ EDUC,IL-31999 HAIFA,ISRAEL. RP MIRSKY, AF (reprint author), NIMH,PSYCHOL & PSYCHOPATHOL LAB,BLDG 10,ROOM 4C-110,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 75 TC 39 Z9 39 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 227 EP 239 PG 13 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200008 PM 7631170 ER PT J AU SHORE, D MATTHEWS, S COTT, J LIEBERMAN, JA AF SHORE, D MATTHEWS, S COTT, J LIEBERMAN, JA TI CLINICAL IMPLICATIONS OF CLOZAPINE DISCONTINUATION - REPORT OF AN NIMH WORKSHOP SO SCHIZOPHRENIA BULLETIN LA English DT Editorial Material ID WITHDRAWAL AB In September 1994, the National Institute of Mental Health convened a group of scientists to discuss the clinical effects of rapid clozapine discontinuation, especially in light of the introduction of risperidone for the treatment of schizophrenia. Despite concern over recent reports of clinical deterioration (psychotic exacerbations, somatic withdrawal symptoms, and extrapyramidal side effects) in a few patients abruptly discontinued from clozapine, there is currently insufficient information to determine the magnitude of the problems associated with clozapine withdrawal. However, clinicians are reminded that the withdrawal schedule for clozapine indicates a gradual tapering schedule (unless the patient is experiencing severe side effects): that switching patients from clozapine to risperidone does not mean that such tapering is unnecessary; and that the use of risperidone may not produce all of the same effects as clozapine in some treatment-refractory patients. C1 NIMH,DIV CLIN & TREATMENT RES,CLIN TREATMENT RES BRANCH,PHARMACOL TREATMENT RES PROGRAM,ROCKVILLE,MD 20857. ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,GLEN OAKS,NY. RP SHORE, D (reprint author), NIMH,DIV CLIN & TREATMENT RES,RES ACTIV,PARKLAWN BLDG,ROOM 18C-26,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 11 TC 45 Z9 46 U1 0 U2 0 PU US GOVERNMENT PRINTING OFFICE PI WASHINGTON PA SUPT OF DOCUMENTS, WASHINGTON, DC 20402-9325 SN 0586-7614 J9 SCHIZOPHRENIA BULL JI Schizophr. Bull. PY 1995 VL 21 IS 2 BP 333 EP 338 PG 6 WC Psychiatry SC Psychiatry GA QX822 UT WOS:A1995QX82200018 PM 7543218 ER PT J AU PRELL, GD GREEN, JP KAUFMANN, CA KHANDELWAL, JK MORRISHOW, AM KIRCH, DG LINNOILA, M WYATT, RJ AF PRELL, GD GREEN, JP KAUFMANN, CA KHANDELWAL, JK MORRISHOW, AM KIRCH, DG LINNOILA, M WYATT, RJ TI HISTAMINE METABOLITES IN CEREBROSPINAL-FLUID OF PATIENTS WITH CHRONIC-SCHIZOPHRENIA - THEIR RELATIONSHIPS TO LEVELS OF OTHER AMINERGIC TRANSMITTERS AND RATINGS OF SYMPTOMS SO SCHIZOPHRENIA RESEARCH LA English DT Article DE HISTAMINE METABOLITE; CEREBROSPINAL FLUID; (SCHIZOPHRENIA) ID PROS-METHYLIMIDAZOLEACETIC ACID; CHROMATOGRAPHY-MASS SPECTROMETRY; RAT-BRAIN; TELE-METHYLHISTAMINE; CONCENTRATION GRADIENTS; REGIONAL DISTRIBUTION; N-METHYLTRANSFERASE; RHESUS-MONKEY; MAO-B; FAMOTIDINE AB Levels of the histamine metabolites, tele-methylhistamine (t-MH) and tele-methylimidazoleacetic acid (t-MIAA), and metabolites of other aminergic transmitters and of norepinephrine were measured in cerebrospinal fluid of 36 inpatients with chronic schizophrenia and eight controls. The mean t-MH level from controls was nearly identical to the levels seen previously in healthy volunteers. Compared with controls, the mean level of t-MH in the schizophrenic patients was 12.6-fold (higher (p=0.006); 21 of the patients had levels exceeding the range of controls. There was no significant difference (p>0.05) in levels of other analytes, although the levels of t-MH correlated significantly with those of t-MIAA, homovanillic acid, 3,4-dihydroxyphenylacetic acid, norepinephrine, 3-methoxy-4-hydroxyphenylglycol and 5-hydroxyindoleacetic acid. The difference in levels of t-MH were not attributable to medication, since those taking (n=10) or withdrawn from (n=26) neuroleptic drugs had nearly the same mean levels of t-MH; each group had higher levels than controls (ANOVA: p<0.05). Patients with or without tardive dyskinesia showed no significant differences in means of any analyte. Only levels of t-MH-among those with schizophrenia correlated with positive symptom scores on the Psychiatric Symptom Assessment Scale (r(s)=0.45, p<0.02). The elevated levels of t-MH in cerebrospinal fluid, which represent histamine that was released and metabolized, suggest increased central histaminergic activity in patients with chronic schizophrenia. C1 CUNY MT SINAI SCH MED,DEPT PHARMACOL,NEW YORK,NY 10029. ST ELIZABETH HOSP,NIMH,NEUROPSYCHIAT BRANCH,WASHINGTON,DC 20032. NIAAA,DIV INTRAMURAL CLIN & BIOL RES,BETHESDA,MD 20205. FU NIDA NIH HHS [5-T32-DA-07135]; NIMH NIH HHS [MH-31805]; NINDS NIH HHS [NS-28012] NR 96 TC 91 Z9 94 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD JAN PY 1995 VL 14 IS 2 BP 93 EP 104 DI 10.1016/0920-9964(94)00034-6 PG 12 WC Psychiatry SC Psychiatry GA QB710 UT WOS:A1995QB71000001 PM 7711000 ER PT J AU RACIOPPI, L GERMAIN, RN AF RACIOPPI, L GERMAIN, RN TI MODIFIED T-CELL RECEPTOR LIGANDS - MOVING BEYOND A STRICT OCCUPANCY MODEL FOR T-CELL ACTIVATION BY ANTIGEN SO SELECTIVE IMMUNOSUPPRESSION: BASIC CONCEPTS AND CLINICAL APPLICATIONS SE CHEMICAL IMMUNOLOGY LA English DT Review ID TYROSINE PHOSPHORYLATION; COSTIMULATORY SIGNAL; JUNCTIONAL REGIONS; SURFACE MOLECULES; THYMIC SELECTION; FINE SPECIFICITY; PRESENTING CELLS; MHC MOLECULES; C ACTIVATION; RECOGNITION C1 NIAID,IMMUNOL LAB,LYMPHOCYTE BIOL SECT,BETHESDA,MD 20892. RP RACIOPPI, L (reprint author), UNIV NAPLES FEDERICO II,DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC,IMMUNOL LAB,I-80131 NAPLES,ITALY. NR 71 TC 7 Z9 7 U1 1 U2 1 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 1015-0145 J9 CHEM IMMUNOL JI Chem.Immunol. PY 1995 VL 60 BP 79 EP 99 PG 21 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA BC15N UT WOS:A1995BC15N00007 PM 7857530 ER PT J AU ZBAR, B AF ZBAR, B TI GENETIC TECHNIQUES IN THE DIAGNOSIS OF CARCINOMAS OF THE KIDNEY SO SEMINARS IN NEPHROLOGY LA English DT Article ID VONHIPPEL-LINDAU DISEASE; RENAL-CELL CARCINOMAS; TRANSLOCATION; PAPILLARY; CANCER RP ZBAR, B (reprint author), NCI,FREDERICK CANC RES & DEV CTR,IMMUNOBIOL LAB,BLDG 560,RM 12071,FREDERICK,MD 21702, USA. NR 28 TC 1 Z9 1 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9295 J9 SEMIN NEPHROL JI Semin. Nephrol. PD JAN PY 1995 VL 15 IS 1 BP 50 EP 56 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA QF380 UT WOS:A1995QF38000006 PM 7754256 ER PT J AU Burchinal, LG Lukas, D AF Burchinal, LG Lukas, D TI Periodicals for librarians and information professionals in Central Europe SO SERIALS LIBRARIAN LA English DT Article AB In May 1994, ASSIST INTERNATIONAL INC. began the Cooperative Periodical Program, which provides for delivery of donated copies of 25 leading American library and information science periodicals to 50 libraries in 13 newly independent countries of central Europe and the former Soviet Union. The periodicals are donated by 14 publishers. Shipment and other costs are covered by contributions from firms and organizations that advertise in the periodicals. Librarians report that the periodicals are the most important source of information about library and information services available to information professionals in their countries. C1 NATL LIB MED,BETHESDA,MD 20894. RP Burchinal, LG (reprint author), ASSIST INT INC,4141 N HENDERSON RD 1216,ARLINGTON,VA 22203, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0361-526X J9 SERIALS LIBR JI Ser. Libr. PY 1995 VL 27 IS 4 BP 79 EP 85 PG 7 WC Information Science & Library Science SC Information Science & Library Science GA UJ260 UT WOS:A1995UJ26000008 ER PT J AU BOGUSKI, MS AF BOGUSKI, MS TI ADVENTURES IN INFORMATION SPACE - BIOMEDICAL DISCOVERIES IN A MOLECULAR SEQUENCE MILIEU SO SERIALS LIBRARIAN LA English DT Article; Proceedings Paper CT 9th Annual Conference of the North-American-Serials-Interest-Group, Inc - A Kaleidoscope of Choices: Reshaping Roles and Opportunities for Serialists CY JUN 02-05, 1994 CL VANCOUVER, CANADA SP N AMER SERIALS INTEREST GRP INC AB Since the mid-1970s, DNA and protein sequence data has led to remarkable discoveries that are revealing the fundamental causes of cancer and genetic disease. By the year 2005, the Human Genome Project will produce a complete blueprint of human biology. Scientists and physicians access these data in GenBank(R), a NM database developed, maintained and distributed by the NCBI. These sequences are linked directly to molecular structure databases and to the biomedical journal literature through bibliographic databases like MEDLINE(R). This new knowledge management system functions with the use of e-mail, CD-ROM products, and Internet client-server applications, and blends the world of print publishing with primary source data residing in globally accessible databanks. Current systems require that human beings initiate specific search,. analysis, and retrieval requests. However, software robots already maintain and update these data, and ''intelligent agents'' will soon be available for the automated and targeted dissemination of new research findings. This paper discusses the development of this new working model and speculates about the impact of future technological advances, the effect on how researchers and physicians work, and possible applicability to other research disciplines. C1 NATL INST HLTH,NATL CTR HUMAN GENOME RES,BETHESDA,MD. RP BOGUSKI, MS (reprint author), NATL INST HLTH,NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD, USA. NR 11 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0361-526X J9 SERIALS LIBR JI Ser. Libr. PY 1995 VL 25 IS 3-4 BP 125 EP 131 DI 10.1300/J123v25n03_15 PG 7 WC Information Science & Library Science SC Information Science & Library Science GA QQ944 UT WOS:A1995QQ94400015 ER PT J AU WEISS, PJ AF WEISS, PJ TI GETTING THE EXPERT INTO THE SYSTEM - EXPERT-SYSTEMS AND CATALOGING SO SERIALS LIBRARIAN LA English DT Article; Proceedings Paper CT 9th Annual Conference of the North-American-Serials-Interest-Group, Inc - A Kaleidoscope of Choices: Reshaping Roles and Opportunities for Serialists CY JUN 02-05, 1994 CL VANCOUVER, CANADA SP N AMER SERIALS INTEREST GRP INC AB The author provides an overview of the potential value expert systems have for serials cataloging. Criteria for determining the suitability of a project for use with an expert system are provided and four technical services examples are discussed. RP WEISS, PJ (reprint author), NATL LIB MED,DIV TECH SERV,OFF CHIEF,BETHESDA,MD, USA. NR 1 TC 1 Z9 1 U1 0 U2 2 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0361-526X J9 SERIALS LIBR JI Ser. Libr. PY 1995 VL 25 IS 3-4 BP 235 EP 241 DI 10.1300/J123v25n03_25 PG 7 WC Information Science & Library Science SC Information Science & Library Science GA QQ944 UT WOS:A1995QQ94400025 ER PT B AU PATTATUCCI, AML HAMER, DH AF PATTATUCCI, AML HAMER, DH BE Abramson, PR Pinkerton, SD TI The genetics of sexual orientation: From fruit flies to humans SO SEXUAL NATURE, SEXUAL CULTURE SE CHICAGO SERIES ON SEXUALITY, HISTORY, AND SOCIETY LA English DT Proceedings Paper CT Wenner-Gren Conference on Theorizing Sexuality - Evolution, Culture, and Development CY MAR 19-27, 1993 CL CASCAIS, PORTUGAL SP Wenner Gren Fdn RP NCI,BETHESDA,MD 20892, USA. NR 0 TC 1 Z9 1 U1 1 U2 15 PU UNIV CHICAGO PRESS PI CHICAGO PA 5801 S ELLIS AVE, CHICAGO, IL 60637 BN 0-226-00181-4 J9 CHIC SEX HIST SOC PY 1995 BP 154 EP 174 PG 21 WC Psychology SC Psychology GA BD37Y UT WOS:A1995BD37Y00008 ER PT J AU MORRISON, DK AF MORRISON, DK TI ACTIVATION OF RAF-1 BY RAS IN INTACT-CELLS SO SMALL GTPASES AND THEIR REGULATORS, PT A SE METHODS IN ENZYMOLOGY LA English DT Review ID KINASE; PHOSPHORYLATION; SIGNALS; COMPLEX RP MORRISON, DK (reprint author), NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,CELLULAR GROWTH MECHANISMS GRP,FREDERICK,MD 21702, USA. FU NCI NIH HHS [N01-CO-74101] NR 21 TC 18 Z9 18 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 255 BP 301 EP 310 PG 10 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE11X UT WOS:A1995BE11X00031 PM 8524115 ER PT J AU MIKI, T AF MIKI, T TI INTERACTION OF ECT2 AND DBL WITH RHO-RELATED GTPASES SO SMALL GTPASES AND THEIR REGULATORS, PT B SE METHODS IN ENZYMOLOGY LA English DT Review ID EXPRESSION CDNA CLONING; NUCLEOTIDE EXCHANGE; MOLECULAR-CLONING; BCR GENE; ONCOGENE; PROTEIN; PRODUCT; ENCODES; DOMAIN; YEAST RP MIKI, T (reprint author), NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,BETHESDA,MD 20892, USA. NR 25 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 256 BP 90 EP 98 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE11Y UT WOS:A1995BE11Y00011 PM 7476459 ER PT J AU ZANGRILLI, D EVA, A AF ZANGRILLI, D EVA, A TI CELL-TRANSFORMATION BY DBL ONCOGENE SO SMALL GTPASES AND THEIR REGULATORS, PT B SE METHODS IN ENZYMOLOGY LA English DT Review ID PROTO-ONCOGENE; PRODUCT; FIBROBLASTS; LYMPHOMA; REGION; ASSAY RP ZANGRILLI, D (reprint author), NCI,CELLULAR & MOLEC BIOL LAB,BLDG 37,BETHESDA,MD 20892, USA. RI Eva, Alessandra/J-8268-2016 OI Eva, Alessandra/0000-0003-2949-078X NR 19 TC 9 Z9 9 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 256 BP 347 EP 358 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE11Y UT WOS:A1995BE11Y00038 PM 7476452 ER PT J AU RANDAZZO, PA WEISS, O KAHN, RA AF RANDAZZO, PA WEISS, O KAHN, RA TI PREPARATION OF RECOMBINANT ADP-RIBOSYLATION FACTOR SO SMALL GTPASES AND THEIR REGULATORS, PT C SE METHODS IN ENZYMOLOGY LA English DT Review ID GTP-BINDING-PROTEIN; CHOLERA-TOXIN; REGULATORY COMPONENT; ADENYLATE-CYCLASE; NUCLEOTIDE-BINDING; RNA-POLYMERASE; EXPRESSION; COFACTOR; BOVINE; GENES C1 HADASSAH UNIV HOSP,DEPT ENDOCRINOL & METAB,IL-91120 JERUSALEM,ISRAEL. RP RANDAZZO, PA (reprint author), NCI,DIV CANC TREATMENT,BIOL CHEM LAB,BETHESDA,MD 20892, USA. NR 28 TC 43 Z9 43 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0076-6879 J9 METHOD ENZYMOL JI Methods Enzymol. PY 1995 VL 257 BP 128 EP 135 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BE08F UT WOS:A1995BE08F00016 PM 8583914 ER PT J AU ASHERY, RS CARLSON, RG FALCK, RS SIEGAL, HA AF ASHERY, RS CARLSON, RG FALCK, RS SIEGAL, HA TI INJECTION-DRUG USERS, CRACK-COCAINE USERS, AND HUMAN-SERVICES UTILIZATION - AN EXPLORATORY-STUDY SO SOCIAL WORK LA English DT Article DE AIDS; DRUG ABUSERS; FOCUS GROUPS; SERVICE UTILIZATION; SOCIAL SERVICES SYSTEM ID AIDS AB Estimates of the number of people addicted to heroin and cocaine run into the millions. How these drug abusers interact with the social services system is not well understood. To gain insight into the nature and extent of such interactions, an exploratory study was conducted to gather information on the perceptions and utilization of human services by 44 drug abusers not in treatment. Twenty-nine injection drug users and 15 crack-cocaine users participated in focus group sessions and structured interviews. Participants were recruited by indigenous outreach workers in Dayton and Columbus, Ohio. Findings revealed a very high rate of service use by the drug users. The results raise questions about the role and efficacy of the social services system in identifying drug users and addressing their needs. In addition, the findings raise perplexing questions regarding the effectiveness of acquired immune deficiency syndrome risk-reduction efforts among injection drug users and crack-cocaine users. C1 WRIGHT STATE UNIV,SCH MED,DEPT COMMUNITY HLTH,DAYTON,OH 45435. NIDA,COMMUNITY RES BRANCH,ROCKVILLE,MD. WRIGHT STATE UNIV,SCH MED,DAYTON COLUMBUS AIDS PREVENT PROJECT,DAYTON,OH 45435. NR 16 TC 11 Z9 11 U1 0 U2 1 PU NATL ASSOC SOCIAL WORKERS PI WASHINGTON PA 750 FIRST ST, NE, STE 700, WASHINGTON, DC 20002-4241 SN 0037-8046 J9 SOC WORK JI Soc. Work PD JAN PY 1995 VL 40 IS 1 BP 75 EP 82 PG 8 WC Social Work SC Social Work GA QM119 UT WOS:A1995QM11900010 PM 7863375 ER PT J AU WILLIAMS, JK AF WILLIAMS, JK TI AFRO-AMERICAN WOMEN LIVING WITH HIV-INFECTION - SPECIAL THERAPEUTIC INTERVENTIONS FOR A GROWING POPULATION SO SOCIAL WORK IN HEALTH CARE LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; PSYCHOSOCIAL ISSUES; AIDS RISK; BLACK RP WILLIAMS, JK (reprint author), NIH,HIV COUNSELING PROGRAM,BETHESDA,MD 20892, USA. NR 30 TC 3 Z9 3 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0098-1389 J9 SOC WORK HEALTH CARE JI Soc. Work Health Care PY 1995 VL 21 IS 2 BP 41 EP 53 DI 10.1300/J010v21n02_03 PG 13 WC Social Work SC Social Work GA TC005 UT WOS:A1995TC00500003 PM 8553198 ER PT J AU Byar, DP Freedman, LS Herzberg, AM AF Byar, DP Freedman, LS Herzberg, AM TI Identifying which sets of parameters are simultaneously estimable in an incomplete factorial design SO STATISTICIAN LA English DT Article DE aliasing; experimental design; missing cells; null space ID RANDOMIZED CLINICAL-TRIALS AB Incomplete factorial designs are fractional or complete factorials with some cells missing. In clinical trials or laboratory experiments that use these designs, not all the main effects and interactions can be estimated. By inspecting the null space of the design matrix one may identify the sets of parameters that can be estimated. Some examples are presented. C1 NCI,DIV CANC PREVENT & CONTROL,BIOMETRY BRANCH,BETHESDA,MD 20892. QUEENS UNIV,KINGSTON,ON,CANADA. NR 13 TC 0 Z9 0 U1 1 U2 1 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD, OXON, ENGLAND OX4 1JF SN 0039-0526 J9 STATISTICIAN JI Statistician PY 1995 VL 44 IS 4 BP 451 EP 456 DI 10.2307/2348894 PG 6 WC Statistics & Probability SC Mathematics GA TL070 UT WOS:A1995TL07000004 ER PT J AU LUM, CK MOHANDAS, N AF LUM, CK MOHANDAS, N TI WORKSHOP ON SIGNAL-TRANSDUCTION IN HEMATOPOIETIC-CELLS SO STEM CELLS LA English DT Editorial Material C1 NIH,DIV RES GRANTS,HEMATOL STUDY SECT 1,BETHESDA,MD 20892. HOP BICETRE,HEMATOL LAB,LE KREMLIN BICETR,FRANCE. RP LUM, CK (reprint author), WORKSHOP SIGNAL TRANSDUCT HEMATOPOIET CELLS,CARMEL,CA, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ALPHAMED PRESS PI DAYTON PA 4100 S KETTERING BLVD, DAYTON, OH 45439-2092 SN 1066-5099 J9 STEM CELLS JI Stem Cells PD JAN PY 1995 VL 13 IS 1 BP 86 EP 86 PG 1 WC Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology SC Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology GA QD135 UT WOS:A1995QD13500009 ER PT S AU BAMBERGER, CM CHROUSOS, GP AF BAMBERGER, CM CHROUSOS, GP BE Henderson, D Philibert, D Roy, AK Teutsch, G TI THE GLUCOCORTICOID RECEPTOR AND RU-486 IN MAN SO STEROID RECEPTORS AND ANTIHORMONES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Steroid Receptors and Antihormones CY SEP 20-23, 1994 CL DALLAS, TX SP New York Acad Sci ID ANTIPROGESTIN STEROID RU-486; PITUITARY-ADRENAL-FUNCTION; ANTAGONIST RU-486; DNA-BINDING; HORMONE RECEPTORS; CUSHINGS-SYNDROME; PLASMA ACTH; RESISTANCE; EXPRESSION; INHIBITION RP BAMBERGER, CM (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,ROOM 10N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 55 TC 33 Z9 34 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-937-1 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 761 BP 296 EP 310 DI 10.1111/j.1749-6632.1995.tb31385.x PG 15 WC Biochemistry & Molecular Biology; Multidisciplinary Sciences; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Science & Technology - Other Topics; Pharmacology & Pharmacy GA BD46N UT WOS:A1995BD46N00022 PM 7625728 ER PT J AU ARAI, K CHROUSOS, GP AF ARAI, K CHROUSOS, GP TI SYNDROMES OF GLUCOCORTICOID AND MINERALOCORTICOID RESISTANCE SO STEROIDS LA English DT Article; Proceedings Paper CT Satellite Symposium on Aldosterone and Hypertension, to the 15th International-Society-of-Hypertension Congress CY MAR 17-19, 1994 CL LORNE, AUSTRALIA SP INT SOC HYPERTENS CONGRESS DE GLUCOCORTICOID RESISTANCE; ALDOSTERONE RESISTANCE ID PRIMARY CORTISOL RESISTANCE; ALDOSTERONE-RECEPTOR DEFICIENCY; GENE-EXPRESSION; ACTH-SECRETION; PSEUDOHYPOALDOSTERONISM; INHERITANCE; UNRESPONSIVENESS; ABNORMALITIES; FAMILIES; FORMS AB Glucocorticoid resistance results from incomplete but apparently generalized inability of glucocorticoids to exert their effects on their target tissues. The condition is associated with compensatory elevation of circulating ACTH and cortisol, with the former causing excess secretion of both adrenal androgens and adrenal steroid biosynthesis intermediates with salt-retaining activity. The manifestations of glucocorticoid resistance vary from asymptomatic to different degrees of hypertension and/or hypokalemic alkalosis and/or hyperandrogenism, caused by elevation cortisol and other salt-retaining steroids, and of adrenal androgens, respectively. In women, hyperandrogenism can result in acne, hirsutism, male type baldness, menstrual irregularities, oligoanovulation, and infertility; in men, it may lead to infertility; and in children to precocious puberty. Different molecular defects, such as point mutations or microdeletions of the highly conserved glucocorticoid receptor gene, alter the functional characteristics or concentrations of the intracellular receptor and cause glucocorticoid resistance. The extreme variability in the clinical manifestations of glucocorticoid resistance and ifs mimicry of many common diseases can be explained by different degrees of glucocorticoid resistance, differing sensitivity of target tissues to mineralocorticoids and/or androgens or both, and perhaps different biochemical defects of the glucocorticoid receptor. Mineralocorticoid resistance results from the inability of aldosterone to exert its effect on target tissues. The syndrome is associated with sail loss, hypotension, and hyperkalemic acidosis. We have cloned and sequenced the cDNA of five unrelated patients with this syndrome and have not found any mutations of pathophysiological significance that would explain the resistance of these patients to aldosterone. In the first of these patients, in whom we demonstrated an excellent therapeutic response to carbenoxolone and fludrocortisone, we also examined 0.9 kb of the 5' regulatory region and the first untranslated exon of the mineralocorticoid receptor gene, without detecting any abnormalities. We suggest that it is possible that the defect responsible for pseudohypoaldosteronism is at a postreceptor level. RP ARAI, K (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,BLDG 10,ROOM 10N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 51 TC 33 Z9 34 U1 0 U2 1 PU BUTTERWORTH-HEINEMANN PI WOBURN PA 225 WILDWOOD AVE #UNITB PO BOX 4500, WOBURN, MA 01801-2084 SN 0039-128X J9 STEROIDS JI Steroids PD JAN PY 1995 VL 60 IS 1 BP 173 EP 179 DI 10.1016/0039-128X(94)00007-Y PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA QJ225 UT WOS:A1995QJ22500034 PM 7792808 ER PT S AU Stratakis, CA Chrousos, GP AF Stratakis, CA Chrousos, GP BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Neuroendocrinology and pathophysiology of the stress system SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING HORMONE; PITUITARY-ADRENAL AXIS; BODY-FAT DISTRIBUTION; CUSHINGS-SYNDROME; SECRETION INVITRO; LOCUS COERULEUS; DEPRESSION; DISEASE; EXERCISE; SYMPTOMS C1 GEORGETOWN UNIV,CHILDRENS MED CTR,DEPT PEDIAT,DIV GENET,WASHINGTON,DC 20007. RP Stratakis, CA (reprint author), NICHHD,NIH,DEV ENDOCRINOL BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 10N262,BETHESDA,MD 20892, USA. NR 72 TC 276 Z9 285 U1 1 U2 25 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 1 EP 18 DI 10.1111/j.1749-6632.1995.tb44666.x PG 18 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00001 PM 8597390 ER PT S AU Kopin, IJ AF Kopin, IJ BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Definitions of stress and sympathetic neuronal responses SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD AB The origin of the concept of stress in biology, intuitively borrowed from the physical definition of stress as a deforming force, is unknown, but well over a century ago the term was used with reference to factors that disrupt normal physiological or psychological function. A newer concept of stress was conceived by Hans Selye, who did much to popularize the use of the term as a state in which demands of life have produced a stereotypic syndrome with broad biological effects, including disease. A field of stress research has emerged in which there have been both semantic and substantive scientific controversies regarding Selye's concepts. In this brief review, I will describe the evolution of thought about biological stress, discuss the similarities and differences between physical and biological stresses and strains, and describe a strategy for testing Selye's hypotheses regarding a single response pattern common to all stresses. RP Kopin, IJ (reprint author), NINCDS,NIH,CLIN NEUROSCI BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 5N214,BETHESDA,MD 20892, USA. NR 6 TC 46 Z9 47 U1 3 U2 14 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 19 EP 30 DI 10.1111/j.1749-6632.1995.tb44667.x PG 12 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00002 PM 8597398 ER PT S AU Carter, CS DeVries, AC Taymans, SE Roberts, RL Williams, JR Chrousos, G AF Carter, CS DeVries, AC Taymans, SE Roberts, RL Williams, JR Chrousos, G BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Adrenocorticoid hormones and the development and expression of mammalian monogamy SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID VOLES MICROTUS-OCHROGASTER; FEMALE PRAIRIE VOLES; RAT; CORTICOSTERONE; SEPARATION; BEHAVIOR; MONKEYS; SYSTEM; BRAIN C1 NICHHD,NIH,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP Carter, CS (reprint author), UNIV MARYLAND,DEPT ZOOL,COLLEGE PK,MD 20742, USA. FU NIMH NIH HHS [MH 01050, MH 45836] NR 34 TC 14 Z9 14 U1 1 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 82 EP 91 DI 10.1111/j.1749-6632.1995.tb44672.x PG 10 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00007 PM 8597447 ER PT S AU Eskay, RL Chautard, T Torda, T Daoud, RI Hamelink, C AF Eskay, RL Chautard, T Torda, T Daoud, RI Hamelink, C BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Alcohol, corticosteroids, energy utilization, and hippocampal endangerment SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID PITUITARY-ADRENAL AXIS; PROLONGED ETHANOL-CONSUMPTION; MORPHOLOGICAL ALTERATIONS; CHRONIC EXPOSURE; RAT; STRESS; GLUCOCORTICOIDS; IMPAIRMENT; WITHDRAWAL; ADAPTATION RP Eskay, RL (reprint author), NIAAA,DICBR,CLIN STUDIES LAB,SECT NEUROCHEM & NEUROENDOCRINOL,NIH BLDG 10,ROOM 3C103,BETHESDA,MD 20892, USA. NR 51 TC 18 Z9 18 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 105 EP 114 DI 10.1111/j.1749-6632.1995.tb44674.x PG 10 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00009 PM 8597391 ER PT S AU Pacak, K Palkovits, M Kvetnansky, RA Yadid, G Kopin, IJ Goldstein, DS AF Pacak, K Palkovits, M Kvetnansky, RA Yadid, G Kopin, IJ Goldstein, DS BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Effects of various stressors an in vivo norepinephrine release in the hypothalamic paraventricular nucleus and on the pituitary-adrenocortical axis SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID VENTRAL NORADRENERGIC BUNDLE; SYMPATHETIC-NERVE ACTIVITY; HYPOPHYSEAL PORTAL BLOOD; INVIVO MEASUREMENT; INDUCED ACTIVATION; MONOAMINE-OXIDASE; RAT HYPOTHALAMUS; PLASMA-LEVELS; CATECHOLAMINES; CORTICOTROPIN C1 NIMH,CELL BIOL LAB,NIH,BETHESDA,MD 20892. SLOVAK ACAD SCI,INST EXPTL ENDOCRINOL,BRATISLAVA,SLOVAKIA. BAR ILAN UNIV,DEPT LIFE SCI,RAMAT GAN,ISRAEL. RP Pacak, K (reprint author), NINCDS,NIH,CLIN NEUROSCI BRANCH,9000 ROCKVILLE PIKE,BLDG 10,ROOM 5N214,BETHESDA,MD 20892, USA. RI Palkovits, Miklos/F-2707-2013 NR 45 TC 112 Z9 114 U1 0 U2 7 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 115 EP 130 DI 10.1111/j.1749-6632.1995.tb44675.x PG 16 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00010 PM 8597392 ER PT S AU Kvetnansky, R Pacak, K Fukuhara, K Viskupic, E Hiremagalur, B Nankova, B Goldstein, DS Sabban, EL Kopin, IJ AF Kvetnansky, R Pacak, K Fukuhara, K Viskupic, E Hiremagalur, B Nankova, B Goldstein, DS Sabban, EL Kopin, IJ BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Sympathoadrenal system in stress - Interaction with the hypothalamic-pituitary-adrenocortical system SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID PHENYLETHANOLAMINE N-METHYLTRANSFERASE; CORTICOTROPIN-RELEASING-FACTOR; DOPAMINE-BETA-HYDROXYLASE; REPEATED IMMOBILIZATION STRESS; SYMPATHETIC NERVOUS-SYSTEM; PNMT MESSENGER-RNA; RAT ADRENAL-GLAND; TRANS-SYNAPTIC INDUCTION; TYROSINE-HYDROXYLASE; ADRENALECTOMIZED RATS C1 NINCDS,NIH,BETHESDA,MD. NEW YORK MED COLL,VALHALLA,NY 10595. RP Kvetnansky, R (reprint author), SLOVAK ACAD SCI,INST EXPTL ENDOCRINOL,VLARSKA 3,BRATISLAVA 83306,SLOVAKIA. FU FIC NIH HHS [TW-00108] NR 141 TC 156 Z9 158 U1 0 U2 6 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 131 EP 158 DI 10.1111/j.1749-6632.1995.tb44676.x PG 28 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00011 PM 8597393 ER PT S AU Aguilera, G Kiss, A Luo, X Akbasak, BS AF Aguilera, G Kiss, A Luo, X Akbasak, BS BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI The renin angiotensin system and the stress response SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING FACTOR; SYMPATHETIC NERVOUS-SYSTEM; II RECEPTOR SUBTYPES; PARAVENTRICULAR NUCLEUS; RAT-BRAIN; SUBSTANCE-P; SECRETION; PITUITARY; HYPOTHALAMUS; VASOPRESSIN RP Aguilera, G (reprint author), NICHHD,NIH,DEV ENDOCRINOL BRANCH,SECT ENDOCRINE PHYSIOL,BETHESDA,MD 20892, USA. NR 41 TC 39 Z9 40 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 173 EP 186 DI 10.1111/j.1749-6632.1995.tb44679.x PG 14 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00014 PM 8597396 ER PT S AU Smith, MA Makino, S Kvetnansky, R Post, RM AF Smith, MA Makino, S Kvetnansky, R Post, RM BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Effects of stress on neurotrophic factor expression in the rat brain SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID PYRAMIDAL NEURONS; GLUCOCORTICOIDS; DEFICITS RP Smith, MA (reprint author), NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,ROOM 3N212,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 21 TC 172 Z9 177 U1 0 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 234 EP 239 DI 10.1111/j.1749-6632.1995.tb44684.x PG 6 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00019 PM 8597402 ER PT S AU Lolait, SJ OCarroll, AM Brownstein, MJ AF Lolait, SJ OCarroll, AM Brownstein, MJ BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Molecular biology of vasopressin receptors SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID NEPHROGENIC DIABETES-INSIPIDUS; MESSENGER-RIBONUCLEIC-ACID; HUMAN ANTIDIURETIC-HORMONE; ANTERIOR-PITUITARY-CELLS; PREGNANT RAT MYOMETRIUM; AFFINITY BINDING-SITES; XENOPUS-LAEVIS OOCYTES; CENTRAL-NERVOUS-SYSTEM; OXYTOCIN RECEPTORS; ARGININE VASOPRESSIN RP Lolait, SJ (reprint author), NIMH,CELL BIOL LAB,BLDG 36,ROOM 3A-17,36 CONVENT DR MSC 4090,BETHESDA,MD 20892, USA. RI Brownstein, Michael/B-8609-2009 NR 119 TC 51 Z9 51 U1 1 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 273 EP 292 DI 10.1111/j.1749-6632.1995.tb44688.x PG 20 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00023 PM 8597406 ER PT S AU Palkovits, M Baffi, JS Dvori, S AF Palkovits, M Baffi, JS Dvori, S BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Neuronal organization of stress response - Pain-induced c-fos expression in brain stem catecholaminergic cell groups SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID LATERAL RETICULAR NUCLEUS; SUPERFICIAL DORSAL HORN; CORTICOTROPIN-RELEASING FACTOR; VENTRAL NORADRENERGIC BUNDLE; RAT SPINAL-CORD; PARAVENTRICULAR NUCLEUS; SUPRAOPTIC NUCLEI; IMMOBILIZATION STRESS; HORSERADISH-PEROXIDASE; NOXIOUS-STIMULATION C1 SEMMELWEIS UNIV MED,SCH MED,NEUROMORPHOL LAB,H-1450 BUDAPEST,HUNGARY. RP Palkovits, M (reprint author), NIMH,CELL BIOL LAB,BLDG 36,ROOM 3A17,BETHESDA,MD 20892, USA. RI Palkovits, Miklos/F-2707-2013; OI Palkovits, Miklos/0000-0003-0578-0387 NR 49 TC 32 Z9 33 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 313 EP 326 DI 10.1111/j.1749-6632.1995.tb44691.x PG 14 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00026 PM 8597410 ER PT S AU Sabban, EL Hiremagalur, B Nankova, B Kvetnansky, R AF Sabban, EL Hiremagalur, B Nankova, B Kvetnansky, R BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Molecular biology of stress-elicited induction of catecholamine biosynthetic enzymes SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID DOPAMINE-BETA-HYDROXYLASE; REPEATED IMMOBILIZATION STRESS; PHENYLETHANOLAMINE N-METHYLTRANSFERASE; ADRENAL TYROSINE-HYDROXYLASE; COLD-INDUCED ALTERATIONS; MESSENGER-RNA; PC12 CELLS; GENE; EXPRESSION; RATS C1 NINDS,NIH,BETHESDA,MD 20892. SLOVAK ACAD SCI,INST EXPTL ENDOCRINOL,BRATISLAVA,SLOVAKIA. RP Sabban, EL (reprint author), NEW YORK MED COLL,DEPT BIOCHEM & MOLEC BIOL,VALHALLA,NY 10595, USA. FU FIC NIH HHS [TW00108]; NINDS NIH HHS [NS28869] NR 34 TC 46 Z9 48 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 327 EP 338 DI 10.1111/j.1749-6632.1995.tb44692.x PG 12 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00027 PM 8597411 ER PT S AU Tsigos, C Arai, K Latronico, AC Webster, E Chrousos, GP AF Tsigos, C Arai, K Latronico, AC Webster, E Chrousos, GP BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Receptors for melanocortin peptides in the hypothalamic-pituitary-adrenal axis and skin SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID FAMILIAL GLUCOCORTICOID DEFICIENCY; MELANOCYTE-STIMULATING HORMONE; MOLECULAR-CLONING; ADRENOCORTICOTROPIN RECEPTOR; NERVOUS-SYSTEM; GENE; MUTATION; CORTICOTROPIN; MELANOTROPIN; PIGMENTATION RP Tsigos, C (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,NIH,BLDG 10,ROOM 10N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. OI Latronico Xavier, Ana Claudia/0000-0001-6782-693X NR 46 TC 8 Z9 8 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 352 EP 363 DI 10.1111/j.1749-6632.1995.tb44694.x PG 12 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00029 PM 8597413 ER PT S AU Sternberg, EM Licinio, J AF Sternberg, EM Licinio, J BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Overview of neuroimmune stress interactions - Implications for susceptibility to inflammatory disease SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING HORMONE; PITUITARY-ADRENAL AXIS; CENTRAL-NERVOUS-SYSTEM; MESSENGER-RNA; LEWIS RATS; BIOCHEMICAL MANIFESTATIONS; CELL-DEATH; INTERLEUKIN-1; BRAIN; ARTHRITIS RP Sternberg, EM (reprint author), NIMH,CLIN NEUROENDOCRINOL BRANCH,NIH,BLDG 10,ROOM 3S-231,10 CTR DR,MSC 1284,BETHESDA,MD 20892, USA. RI Licinio, Julio/L-4244-2013 OI Licinio, Julio/0000-0001-6905-5884 NR 53 TC 27 Z9 28 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 364 EP 371 DI 10.1111/j.1749-6632.1995.tb44695.x PG 8 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00030 PM 8597414 ER PT S AU Kapcala, LP Chautard, T Eskay, RL AF Kapcala, LP Chautard, T Eskay, RL BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI The protective role of the hypothalamic-pituitary-adrenal axis against lethality produced by immune, infectious, and inflammatory stress SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING FACTOR; TUMOR-NECROSIS-FACTOR; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; INTERLEUKIN-1-BETA MESSENGER-RNA; ADULT-RAT BRAIN; FACTOR-ALPHA; ADRENOCORTICOTROPIN RESPONSE; NEUROENDOCRINE FUNCTIONS; PARAVENTRICULAR NUCLEUS; RECEPTOR ANTAGONIST C1 NIMH,NIAAA,CLIN STUDIES LAB,BETHESDA,MD 20892. RP Kapcala, LP (reprint author), UNIV MARYLAND,SCH MED,DEPT MED,DIV ENDOCRINOL,BALTIMORE,MD 21201, USA. NR 86 TC 73 Z9 74 U1 1 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 419 EP 437 DI 10.1111/j.1749-6632.1995.tb44699.x PG 19 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00034 PM 8597419 ER PT S AU Mastorakos, G Magiakou, MA Chrousos, G AF Mastorakos, G Magiakou, MA Chrousos, G BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Effects of the immune inflammatory reaction on the hypothalamic-pituitary-adrenal axis SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING FACTOR; FREELY-MOVING RATS; MEDIAN-EMINENCE; ADRENOCORTICOTROPIC HORMONE; ARGININE-VASOPRESSIN; LOCAL RELEASE; INTERLEUKIN-6; CYTOKINE; SECRETION; IL-6 C1 NICHHD,DEV ENDOCRINOL BRANCH,NIH,BETHESDA,MD 20892. NR 33 TC 20 Z9 22 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 438 EP 448 DI 10.1111/j.1749-6632.1995.tb44700.x PG 11 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00035 PM 8597420 ER PT S AU Crofford, LJ Sano, H Karalis, K Webster, EA Friedman, TC Chrousos, GP Wilder, RL AF Crofford, LJ Sano, H Karalis, K Webster, EA Friedman, TC Chrousos, GP Wilder, RL BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Local expression of corticotropin releasing hormone in inflammatory arthritis SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID PITUITARY-ADRENAL AXIS; TRANSFORMATION-ASSOCIATED METALLOPROTEINASE; NEUROGENIC PLASMA EXTRAVASATION; TRANSIN STROMELYSIN EXPRESSION; PLATELET-ACTIVATING FACTOR; FIBROBLAST GROWTH-FACTOR; CELL-WALL ARTHRITIS; RAT SPINAL-CORD; RHEUMATOID-ARTHRITIS; MESSENGER-RNA C1 KYOTO PREFECTURAL UNIV MED,DEPT INTERNAL MED 1,KAMIGYO KU,KYOTO 602,JAPAN. BOSTON CHILDRENS HOSP,DEPT ENDOCRINOL,BOSTON,MA 02115. NICHHD,DEV NEUROBIOL LAB,BETHESDA,MD 20892. NIAMSD,NIH,ARTHRIT & RHEUMATISM BRANCH,INFLAMMATORY JOINT DIS SECT,BETHESDA,MD 20892. RP Crofford, LJ (reprint author), UNIV MICHIGAN,DIV RHEUMATOL,DEPT INTERNAL MED,200 ZINA PITCHER PL,R 4570 KRESGE 1,ANN ARBOR,MI 48109, USA. RI Crofford, Leslie/J-8010-2013 NR 63 TC 22 Z9 22 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 459 EP 471 DI 10.1111/j.1749-6632.1995.tb44702.x PG 13 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00037 PM 8597422 ER PT S AU Cizza, G Gold, PW Chrousos, GP AF Cizza, G Gold, PW Chrousos, GP BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Aging is associated in the 344/N fischer rat with decreased stress responsivity of central and peripheral catecholaminergic systems and impairment of the hypothalamic-pituitary-adrenal axis SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING HORMONE; REPEATED IMMOBILIZATION STRESS; RECEPTOR MESSENGER-RNA; TYROSINE-HYDROXYLASE; LOCUS COERULEUS; PARAVENTRICULAR NUCLEUS; CORTICOSTERONE RESPONSES; AGED RATS; BRAIN; SECRETION C1 NIMH,CLIN NEUROENDOCRINOL BRANCH,NIH,BETHESDA,MD 20892. RP Cizza, G (reprint author), NICHHD,DEV ENDOCRINOL BRANCH,NIH,BLDG 10,ROOM 10N262,10 CTR DR,BETHESDA,MD 20892, USA. NR 68 TC 35 Z9 35 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 491 EP 511 DI 10.1111/j.1749-6632.1995.tb44705.x PG 21 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00040 PM 8597425 ER PT S AU Reincke, M Lehmann, R Karl, M Magiakou, A Chrousos, GP Allolio, B AF Reincke, M Lehmann, R Karl, M Magiakou, A Chrousos, GP Allolio, B BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Severe illness - Neuroendocrinology SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING HORMONE; PITUITARY-ADRENAL AXIS; TUMOR NECROSIS FACTOR; CRITICALLY ILL PATIENTS; ACUTE MEDICAL ILLNESS; NONTHYROIDAL ILLNESSES; CORTISOL RESPONSE; THYROID-FUNCTION; SEPTIC SHOCK; HYPERRENINEMIC HYPOALDOSTERONISM C1 NICHHD,DEV ENDOCRINOL BRANCH,NIH,BETHESDA,MD 20892. RP Reincke, M (reprint author), UNIV WURZBURG,MED KLIN,JOSEF SCHNEIDER STR 2,D-97080 WURZBURG,GERMANY. NR 56 TC 12 Z9 13 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 556 EP 569 DI 10.1111/j.1749-6632.1995.tb44710.x PG 14 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00045 PM 8597431 ER PT S AU Goldstein, DS AF Goldstein, DS BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Clinical assessment of sympathetic responses to stress SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID INSULIN-INDUCED HYPOGLYCEMIA; PERFUSED RAT-KIDNEY; NERVE ACTIVITY; PLASMA NOREPINEPHRINE; URINARY DOPAMINE; ESSENTIAL-HYPERTENSION; HUMAN-HEART; PLATELET PHENOLSULFOTRANSFERASE; PHYSIOLOGICAL SIGNIFICANCE; CONJUGATED CATECHOLAMINES AB Comprehending roles of catecholaminergic systems in stress responses in humans. requires knowledge about clinical approaches to assess catecholaminergic function. This paper updates these assessment techniques, including neurochemical methods, direct sympathetic nerve recording, power spectral analysis of heart rate variability, and cardiac sympathoneural imaging. RP Goldstein, DS (reprint author), NINCDS,CLIN NEUROSCI BRANCH,NIH,BLDG 10 ROOM 6N252,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 144 TC 16 Z9 16 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 570 EP 593 DI 10.1111/j.1749-6632.1995.tb44711.x PG 24 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00046 PM 8597432 ER PT S AU Rubinow, DR Schmidt, PJ AF Rubinow, DR Schmidt, PJ BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI The neuroendocrinology of menstrual cycle mood disorders SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID CORTICOTROPIN-RELEASING HORMONE; PREMENSTRUAL TENSION SYNDROME; FEMALE RATS; PROLACTIN RESPONSES; THYROID-FUNCTION; STRESS-RESPONSE; SEX-DIFFERENCES; ADRENOCORTICOTROPIN; WOMEN; SYMPTOMS RP Rubinow, DR (reprint author), NIMH,BIOL PSYCHIAT BRANCH,SECT BEHAV ENDOCRINOL,BLDG 10,ROOM 3N238,10 CTR DR MSC 1276,BETHESDA,MD 20892, USA. NR 51 TC 32 Z9 32 U1 3 U2 8 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 648 EP 659 DI 10.1111/j.1749-6632.1995.tb44717.x PG 12 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00052 PM 8597438 ER PT S AU Peeke, PM Chrousos, GP AF Peeke, PM Chrousos, GP BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Chronic stress and the disease state SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID BODY-FAT DISTRIBUTION; CORTICOTROPIN-RELEASING HORMONE; POTENTIAL CLINICAL IMPLICATIONS; DEPENDENT DIABETES-MELLITUS; ADIPOSE-TISSUE DISTRIBUTION; PITUITARY-ADRENAL AXIS; GROWTH-HORMONE; BIOCHEMICAL MANIFESTATIONS; CARDIOVASCULAR-DISEASE; CUSHINGS-DISEASE RP Peeke, PM (reprint author), NIH,DEV ENDOCRINOL BRANCH,BLDG 10,ROOM 10N262,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 56 TC 114 Z9 115 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 665 EP 676 DI 10.1111/j.1749-6632.1995.tb44719.x PG 12 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00054 PM 8597440 ER PT S AU Post, RM Weiss, SRB Smith, M Rosen, J Frye, M AF Post, RM Weiss, SRB Smith, M Rosen, J Frye, M BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Stress, conditioning, and the temporal aspects of affective disorders SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID BIPOLAR DISORDER; C-FOS; CONTINGENT TOLERANCE; GENE-EXPRESSION; FOLLOW-UP; LITHIUM; SENSITIZATION; CARBAMAZEPINE; COCAINE; AMYGDALA RP Post, RM (reprint author), NIMH,BIOL PSYCHIAT BRANCH,BLDG 10,ROOM 3N212,10 CTR DR MSC 1272,BETHESDA,MD 20892, USA. NR 42 TC 29 Z9 29 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 677 EP 696 DI 10.1111/j.1749-6632.1995.tb44720.x PG 20 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00055 PM 8597441 ER PT S AU Altemus, M AF Altemus, M BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Neuropeptides in anxiety disorders - Effects of lactation SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID OBSESSIVE-COMPULSIVE DISORDER; CORTICOTROPIN-RELEASING-FACTOR; DEXAMETHASONE SUPPRESSION TEST; URINARY FREE CORTISOL; POSTTRAUMATIC-STRESS-DISORDER; CEREBROSPINAL-FLUID; PANIC DISORDER; MAJOR DEPRESSION; PARAVENTRICULAR NUCLEI; ARGININE-VASOPRESSIN RP Altemus, M (reprint author), NIMH,CLIN SCI LAB,BLDG 10,ROOM 3D41,10 CTR DR MSC 1264,BETHESDA,MD 20892, USA. NR 80 TC 27 Z9 27 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 697 EP 707 DI 10.1111/j.1749-6632.1995.tb44721.x PG 11 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00056 PM 8597442 ER PT S AU Putnam, FW AF Putnam, FW BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Traumatic stress and pathological dissociation SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID MULTIPLE PERSONALITY-DISORDER; VIETNAM COMBAT VETERANS; CLINICAL PHENOMENOLOGY; OPTICAL-DIFFERENCES; SEXUAL ABUSE; CHILDHOOD; INSTRUMENT; INTERVIEW; IMAGERY; EVENTS RP Putnam, FW (reprint author), NIMH,LAB DEV PSYCHOL,UNIT DISSOCIAT DISORDERS,BLDG 15K,9000 ROCKVILLE PIKE,BETHESDA,MD 20892, USA. NR 62 TC 16 Z9 16 U1 8 U2 16 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 708 EP 715 DI 10.1111/j.1749-6632.1995.tb44722.x PG 8 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00057 PM 8597443 ER PT S AU Gold, PW Licinio, J Wong, ML Chrousos, GP AF Gold, PW Licinio, J Wong, ML Chrousos, GP BE Chrousos, GP McCarty, R Pacak, K Cizza, G Sternberg, E Gold, PW Kvetnansky, R TI Corticotropin releasing hormone in the pathophysiology of melancholic and atypical depression and in the mechanism of action of antidepressant drugs SO STRESS: BASIC MECHANISMS AND CLINICAL IMPLICATIONS SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 1st World Congress on Stress CY OCT 02-07, 1994 CL BETHESDA, MD ID PRIMARY ENDOGENOUS-DEPRESSION; NEURO-ENDOCRINE ASPECTS; PITUITARY-ADRENAL AXIS; MAJOR DEPRESSION; DOSE-RESPONSE; PHARMACOKINETIC PROPERTIES; REPRODUCTIVE FUNCTIONS; ANTERIOR-PITUITARY; CORTISOL SECRETION; CUSHINGS-DISEASE C1 NICHHD,INTRAMURAL RES PROGRAM,DEV ENDOCRINOL BRANCH,BETHESDA,MD 20892. RP Gold, PW (reprint author), NIMH,INTRAMURAL RES PROGRAM,CLIN NEUROENDOCRINOL BRANCH,BETHESDA,MD 20892, USA. RI Wong, Ma-Li/D-7903-2011; Licinio, Julio/L-4244-2013 OI Licinio, Julio/0000-0001-6905-5884 NR 53 TC 150 Z9 152 U1 0 U2 4 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-943-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 771 BP 716 EP 729 DI 10.1111/j.1749-6632.1995.tb44723.x PG 14 WC Endocrinology & Metabolism; Multidisciplinary Sciences; Neurosciences SC Endocrinology & Metabolism; Science & Technology - Other Topics; Neurosciences & Neurology GA BE92G UT WOS:A1995BE92G00058 PM 8597444 ER PT J AU TAKAHASHI, H KIRSCH, JR HASHIMOTO, K LONDON, ED KOEHLER, RC TRAYSTMAN, RJ AF TAKAHASHI, H KIRSCH, JR HASHIMOTO, K LONDON, ED KOEHLER, RC TRAYSTMAN, RJ TI A SIGMA-RECEPTOR LIGAND DECREASES BRAIN INJURY FOLLOWING TRANSIENT FOCAL ISCHEMIA IN CAT SO STROKE LA English DT Meeting Abstract C1 JOHNS HOPKINS MED INST,DEPT ANESTHESIOL & CRIT CARE MED,BALTIMORE,MD. NIDA,BALTIMORE,MD. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0039-2499 J9 STROKE JI Stroke PD JAN PY 1995 VL 26 IS 1 BP 181 EP 181 PG 1 WC Clinical Neurology; Peripheral Vascular Disease SC Neurosciences & Neurology; Cardiovascular System & Cardiology GA PZ896 UT WOS:A1995PZ89600164 ER PT S AU PascualLeone, A Grafman, J Hallett, M AF PascualLeone, A Grafman, J Hallett, M BE Grafman, J Holyoak, KJ Boller, F TI Procedural learning and prefrontal cortex SO STRUCTURE AND FUNCTIONS OF THE HUMAN PREFRONTAL CORTEX SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Workshop on Structure and Functions of the Human Prefrontal Cortex CY MAR 02-04, 1995 CL NEW YORK, NY SP New York Acad Sci ID PARKINSONS-DISEASE; KNOWLEDGE C1 INST RAMON Y CAJAL, CONSEJO SUPERIOR INVEST CIENT, VALENCIA, SPAIN. NINCDS, NIH, COGNIT NEUROSCI SECT, BETHESDA, MD 20892 USA. NINCDS, NIH, HUMAN MOTOR CONTROL SECT, BETHESDA, MD 20892 USA. UNIV VALENCIA, DEPT FISIOL, E-46010 VALENCIA, SPAIN. OI Grafman, Jordan H./0000-0001-8645-4457 NR 20 TC 33 Z9 33 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-991-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 769 BP 61 EP 70 DI 10.1111/j.1749-6632.1995.tb38131.x PG 10 WC Multidisciplinary Sciences; Neurosciences; Psychology SC Science & Technology - Other Topics; Neurosciences & Neurology; Psychology GA BE92F UT WOS:A1995BE92F00005 PM 8595044 ER PT S AU Nichelli, P Clark, K Hollnagel, C Grafman, J AF Nichelli, P Clark, K Hollnagel, C Grafman, J BE Grafman, J Holyoak, KJ Boller, F TI Duration processing after frontal lobe lesions SO STRUCTURE AND FUNCTIONS OF THE HUMAN PREFRONTAL CORTEX SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Workshop on Structure and Functions of the Human Prefrontal Cortex CY MAR 02-04, 1995 CL NEW YORK, NY SP New York Acad Sci ID PREFRONTAL CORTEX; MEMORY; BISECTION C1 NINCDS,NIH,COGNIT NEUROSCI SECT,MED NEUROL BRANCH,BETHESDA,MD 20892. RP Nichelli, P (reprint author), UNIV MODENA,NEUROL CLIN,VIA DEL POZZO 71,I-41100 MODENA,ITALY. RI Nichelli, Paolo/F-7336-2015; OI Nichelli, Paolo/0000-0001-9756-6796; Grafman, Jordan H./0000-0001-8645-4457 NR 19 TC 81 Z9 81 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-991-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 769 BP 183 EP 190 DI 10.1111/j.1749-6632.1995.tb38139.x PG 8 WC Multidisciplinary Sciences; Neurosciences; Psychology SC Science & Technology - Other Topics; Neurosciences & Neurology; Psychology GA BE92F UT WOS:A1995BE92F00013 PM 8595025 ER PT S AU Sirigu, A Zalla, T Pillon, B Grafman, J Dubois, B Agid, Y AF Sirigu, A Zalla, T Pillon, B Grafman, J Dubois, B Agid, Y BE Grafman, J Holyoak, KJ Boller, F TI Planning and script analysis following prefrontal lobe lesions SO STRUCTURE AND FUNCTIONS OF THE HUMAN PREFRONTAL CORTEX SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Workshop on Structure and Functions of the Human Prefrontal Cortex CY MAR 02-04, 1995 CL NEW YORK, NY SP New York Acad Sci ID MEMORY C1 ECOLE POLYTECH, CREA, F-75230 PARIS, FRANCE. NIH, COGNIT NEUROSCI SECT, BETHESDA, MD 20892 USA. RP Sirigu, A (reprint author), HOP LA PITIE SALPETRIERE, INSERM, U289, 47 BLVD HOP, F-75013 PARIS, FRANCE. OI Grafman, Jordan H./0000-0001-8645-4457 NR 18 TC 42 Z9 42 U1 1 U2 5 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-991-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 769 BP 277 EP 288 DI 10.1111/j.1749-6632.1995.tb38145.x PG 12 WC Multidisciplinary Sciences; Neurosciences; Psychology SC Science & Technology - Other Topics; Neurosciences & Neurology; Psychology GA BE92F UT WOS:A1995BE92F00019 PM 8595032 ER PT S AU Grafman, J AF Grafman, J BE Grafman, J Holyoak, KJ Boller, F TI Similarities and distinctions among current models of prefrontal cortical functions SO STRUCTURE AND FUNCTIONS OF THE HUMAN PREFRONTAL CORTEX SE Annals of the New York Academy of Sciences LA English DT Review CT Workshop on Structure and Functions of the Human Prefrontal Cortex CY MAR 02-04, 1995 CL NEW YORK, NY SP New York Acad Sci ID FRONTAL-LOBE DAMAGE; SORTING TEST-PERFORMANCE; BEHAVIOR; CORTEX; SCRIPTS; BRAIN; REPRESENTATION; KNOWLEDGE; LESIONS; DISEASE RP Grafman, J (reprint author), NINCDS, MED NEUROL BRANCH,NIH,COGNIT NEUROSCI SECT, 10 CTR DR MSC 1440,BLDG 10, ROOM 5S209, BETHESDA, MD 20892 USA. OI Grafman, Jordan H./0000-0001-8645-4457 NR 115 TC 142 Z9 146 U1 9 U2 12 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 0-89766-991-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 769 BP 337 EP 368 DI 10.1111/j.1749-6632.1995.tb38149.x PG 32 WC Multidisciplinary Sciences; Neurosciences; Psychology SC Science & Technology - Other Topics; Neurosciences & Neurology; Psychology GA BE92F UT WOS:A1995BE92F00023 PM 8595036 ER PT J AU MOSCICKI, EK MUEHRER, P POTTER, LB AF MOSCICKI, EK MUEHRER, P POTTER, LB TI INTRODUCTION TO SUPPLEMENTAL ISSUE - RESEARCH ISSUES IN SUICIDE AND SEXUAL ORIENTATION SO SUICIDE AND LIFE-THREATENING BEHAVIOR LA English DT Editorial Material ID BEHAVIOR C1 CTR DIS CONTROL & PREVENT,NATL CTR INJURY PREVENT & CONTROL,ATLANTA,GA 30341. RP MOSCICKI, EK (reprint author), NIMH,PREVENT RES BRANCH,5600 FISHERS LANE,ROOM 10-85,ROCKVILLE,MD 20857, USA. NR 6 TC 16 Z9 16 U1 0 U2 0 PU GUILFORD PRESS PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0363-0234 J9 SUICIDE LIFE-THREAT JI Suicide Life-Threat. Behav. PY 1995 VL 25 SU S BP 1 EP 3 PG 3 WC Psychiatry; Psychology, Multidisciplinary SC Psychiatry; Psychology GA RY188 UT WOS:A1995RY18800001 PM 8553424 ER PT J AU GRABER, JA BROOKSGUNN, J AF GRABER, JA BROOKSGUNN, J TI MODELS OF DEVELOPMENT - UNDERSTANDING RISK IN ADOLESCENCE SO SUICIDE AND LIFE-THREATENING BEHAVIOR LA English DT Article ID PERSONALITY AB In this paper, we review developmental models for understanding adolescence as they might be applied to emerging sexuality and the challenges and risks that may be associated with different sexual orientations and identities. Models have been identified as: cumulative events or simultaneous change, accentuation, or trajectory models. Inclusion of risk and protective factors and considering the extent of balance between these elements of an adolescent's life is also discussed in terms of predicting mental health outcomes. These models have been particularly useful in the study of the development of psychopathology and clearly relate to the broader issue of understanding suicide risk during adolescence. C1 NICHHD,BETHESDA,MD. RP GRABER, JA (reprint author), COLUMBIA UNIV,COLL TEACHERS,ADOLESCENT STUDY PROGRAM,NEW YORK,NY 10027, USA. FU NICHD NIH HHS [HD32376, HD24770] NR 37 TC 9 Z9 10 U1 2 U2 4 PU GUILFORD PRESS PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0363-0234 J9 SUICIDE LIFE-THREAT JI Suicide Life-Threat. Behav. PY 1995 VL 25 SU S BP 18 EP 25 PG 8 WC Psychiatry; Psychology, Multidisciplinary SC Psychiatry; Psychology GA RY188 UT WOS:A1995RY18800003 PM 8553425 ER PT J AU MUEHRER, P AF MUEHRER, P TI SUICIDE AND SEXUAL ORIENTATION - A CRITICAL SUMMARY OF RECENT RESEARCH AND DIRECTIONS FOR FUTURE-RESEARCH SO SUICIDE AND LIFE-THREATENING BEHAVIOR LA English DT Article ID SOCIAL-SCIENCE PERSPECTIVE; RISK-FACTORS; ADOLESCENT SUICIDE; FOLLOW-UP; BEHAVIOR; GAY; YOUTH; PREVALENCE; PREVENTION; DISORDERS AB Research on the hypothesized relationship between sexual orientation and suicide is limited both in quantity and quality. National or statewide data on the frequency and causes of completed suicide in gay and lesbian people in the general population, including youth, do not exist. Similarly, national or statewide data on the frequency of suicide attempts among the general population or among gay and lesbian people, including youth, do not exist. Methodological limitations in the small research literature include a lack of consensus on definitions for key terms such as suicide attempt and sexual orientation, uncertain reliability and validity of measures for these terms, nonrepresentative samples, and a lack of appropriate nongay and/or nonclinical control groups for making accurate comparisons. These numerous methodological limitations prevent accurate conclusions about the role sexual orientation might play in suicidal behavior; the limitations also suggest opportunities for future research. Furthermore, recent evaluations of some school suicide-awareness programs suggest that these programs are ineffective and may actually have unintended negative effects. The premature dissemination of unproven programs is unwarranted. RP MUEHRER, P (reprint author), NIMH,DIV EPIDEMIOL & SERV RES,PREVENT RES BRANCH,ROOM 10-85,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 42 TC 41 Z9 41 U1 0 U2 1 PU GUILFORD PRESS PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0363-0234 J9 SUICIDE LIFE-THREAT JI Suicide Life-Threat. Behav. PY 1995 VL 25 SU S BP 72 EP 81 PG 10 WC Psychiatry; Psychology, Multidisciplinary SC Psychiatry; Psychology GA RY188 UT WOS:A1995RY18800009 PM 8553431 ER PT J AU ANDERSON, CW CLARK, DC DUNNEMAXIM, K GUSH, C HERBERT, S HUNTER, J KYTLE, R MARIS, R SHAFFER, D SILVERMAN, M BRENT, DA CALLAHAN, J DAUGELLI, AR GRABER, J JACOBY, M MILONAS, A SALTZMAN, LE SHORT, L BARTHOLOW, B BRADFORD, J DOLL, LS GARRISON, CZ GONSIOREK, JC GROSSMAN, J HAYNES, SG KUNREUTHER, F LIEBERMAN, M BRANN, E CABAJ, RP DUNNE, E HALL, J KACHUR, P MOORE, J OLSON, ED PLUMB, M SVED, M VALENTE, SM WOMACK, W AF ANDERSON, CW CLARK, DC DUNNEMAXIM, K GUSH, C HERBERT, S HUNTER, J KYTLE, R MARIS, R SHAFFER, D SILVERMAN, M BRENT, DA CALLAHAN, J DAUGELLI, AR GRABER, J JACOBY, M MILONAS, A SALTZMAN, LE SHORT, L BARTHOLOW, B BRADFORD, J DOLL, LS GARRISON, CZ GONSIOREK, JC GROSSMAN, J HAYNES, SG KUNREUTHER, F LIEBERMAN, M BRANN, E CABAJ, RP DUNNE, E HALL, J KACHUR, P MOORE, J OLSON, ED PLUMB, M SVED, M VALENTE, SM WOMACK, W TI RECOMMENDATIONS FOR A RESEARCH AGENDA IN SUICIDE AND SEXUAL ORIENTATION SO SUICIDE AND LIFE-THREATENING BEHAVIOR LA English DT Article C1 NATL CTR INJURY PREVENT & CONTROL,ATLANTA,GA 30341. RP ANDERSON, CW (reprint author), NIMH,PREVENT RES BRANCH,ROOM 10-85,5600 FISHERS LANE,ROCKVILLE,MD 20857, USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GUILFORD PRESS PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0363-0234 J9 SUICIDE LIFE-THREAT JI Suicide Life-Threat. Behav. PY 1995 VL 25 SU S BP 82 EP 88 PG 7 WC Psychiatry; Psychology, Multidisciplinary SC Psychiatry; Psychology GA RY188 UT WOS:A1995RY18800010 ER PT S AU CONCANNON, P ROBINSON, MA AF CONCANNON, P ROBINSON, MA BE Davis, MM Buxbaum, J TI HUMAN T-CELL RECEPTOR GENE NOMENCLATURE SO T-CELL RECEPTOR USE IN HUMAN AUTOIMMUNE DISEASES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on T-Cell Receptor Use in Human Autoimmune Diseases CY APR 17-20, 1994 CL SAN DIEGO, CA SP Arthritis Fdn, New York Acad Sci ID VARIABLE REGION GENES; BETA-CHAIN GENES; SEQUENCE; ANTIGEN; POLYMORPHISMS; DIVERSITY; SUBFAMILY C1 UNIV WASHINGTON,SCH MED,DEPT IMMUNOL,SEATTLE,WA 98195. NIAID,IMMUNOGENET LAB,TWINBROOK FACIL 2,ROCKVILLE,MD 20852. RP CONCANNON, P (reprint author), VIRGINIA MASON RES CTR,1000 SENECA ST,SEATTLE,WA 98101, USA. OI Concannon, Patrick/0000-0002-5801-1859 NR 22 TC 6 Z9 6 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-915-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 756 BP 124 EP 129 DI 10.1111/j.1749-6632.1995.tb44497.x PG 6 WC Immunology; Multidisciplinary Sciences SC Immunology; Science & Technology - Other Topics GA BD46M UT WOS:A1995BD46M00021 PM 7645818 ER PT S AU MCDERMOTT, M KASTNER, DL HOLLOMAN, JD SCHMIDTWOLF, G LUNDBERG, AS SINHA, AA HSU, C CASHIN, P MOLLOY, MG MULCAHY, B SHANAHAN, F OGARA, F MCCONNELL, FJ ADAMS, C KHAN, MA WOLFF, F RUBIN, LA CLEGG, DO HUSEBYE, D AMOS, CI WARD, RH MCDEVITT, HO AF MCDERMOTT, M KASTNER, DL HOLLOMAN, JD SCHMIDTWOLF, G LUNDBERG, AS SINHA, AA HSU, C CASHIN, P MOLLOY, MG MULCAHY, B SHANAHAN, F OGARA, F MCCONNELL, FJ ADAMS, C KHAN, MA WOLFF, F RUBIN, LA CLEGG, DO HUSEBYE, D AMOS, CI WARD, RH MCDEVITT, HO BE Davis, MM Buxbaum, J TI THE ROLE OF T-CELL RECEPTOR-BETA CHAIN GENES IN THE SUSCEPTIBILITY TO RHEUMATOID-ARTHRITIS SO T-CELL RECEPTOR USE IN HUMAN AUTOIMMUNE DISEASES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on T-Cell Receptor Use in Human Autoimmune Diseases CY APR 17-20, 1994 CL SAN DIEGO, CA SP Arthritis Fdn, New York Acad Sci C1 STANFORD UNIV,DEPT MICROBIOL,STANFORD,CA 94305. STANFORD UNIV,DEPT IMMUNOL,STANFORD,CA 94305. NATL UNIV IRELAND UNIV COLL CORK,DEPT MED,CORK,IRELAND. NATL UNIV IRELAND UNIV COLL CORK,DEPT RHEUMATOL,CORK,IRELAND. NATL UNIV IRELAND UNIV COLL CORK,DEPT MICROBIOL,CORK,IRELAND. CASE WESTERN RESERVE UNIV,DEPT MED,CLEVELAND,OH 44106. UNIV KANSAS,DEPT RHEUMATOL,WICHITA,KS. UNIV TORONTO,DEPT MED,TORONTO,ON,CANADA. UNIV UTAH,DEPT RHEUMATOL,SALT LAKE CITY,UT. UNIV UTAH,DEPT HUMAN GENET,SALT LAKE CITY,UT. UNIV TEXAS,MD ANDERSON CANC CTR,DEPT EPIDEMIOL,HOUSTON,TX. STANFORD UNIV,DEPT MED,STANFORD,CA 94305. RP MCDERMOTT, M (reprint author), NIAMSD,ARTHRITIS & RHEUMATISM BRANCH,BLDG 10,ROOM 9N210,BETHESDA,MD 20892, USA. NR 3 TC 3 Z9 3 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-915-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 756 BP 173 EP 175 DI 10.1111/j.1749-6632.1995.tb44502.x PG 3 WC Immunology; Multidisciplinary Sciences SC Immunology; Science & Technology - Other Topics GA BD46M UT WOS:A1995BD46M00026 PM 7645823 ER PT S AU OHANLON, TP DALAKAS, MC PLOTZ, PH MILLER, FW AF OHANLON, TP DALAKAS, MC PLOTZ, PH MILLER, FW BE Davis, MM Buxbaum, J TI THE ALPHA-BETA-T-CELL RECEPTOR REPERTOIRE IN IDIOPATHIC INFLAMMATORY MYOPATHIES - DISTINCT PATTERNS OF GENE-EXPRESSION BY MUSCLE-INFILTRATING LYMPHOCYTES IN DIFFERENT CLINICAL AND SEROLOGIC GROUPS SO T-CELL RECEPTOR USE IN HUMAN AUTOIMMUNE DISEASES SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on T-Cell Receptor Use in Human Autoimmune Diseases CY APR 17-20, 1994 CL SAN DIEGO, CA SP Arthritis Fdn, New York Acad Sci C1 NIAMSD,BETHESDA,MD 20892. RP OHANLON, TP (reprint author), NINCDS,US FDA,CTR BIOL EVALUAT & RES,MOLEC IMMUNOL LAB,BLDG 298,ROOM 2G11,BETHESDA,MD 20892, USA. NR 6 TC 3 Z9 3 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-915-0 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1995 VL 756 BP 410 EP 413 DI 10.1111/j.1749-6632.1995.tb44548.x PG 4 WC Immunology; Multidisciplinary Sciences SC Immunology; Science & Technology - Other Topics GA BD46M UT WOS:A1995BD46M00072 PM 7645860 ER PT S AU Sher, A Coffman, RL Abbas, AK Liew, FY Trinchieri, G Flavell, R Swain, SL Ramshaw, IA Lotze, MT Mitchison, NA Locksley, RM Mosmann, TR Fitch, FW Allen, PM AF Sher, A Coffman, RL Abbas, AK Liew, FY Trinchieri, G Flavell, R Swain, SL Ramshaw, IA Lotze, MT Mitchison, NA Locksley, RM Mosmann, TR Fitch, FW Allen, PM BE Chadwick, D Cardew, G TI Reversal of polarized T helper 1 and T helper 2 cell populations in murine leishmaniasis - Discussion SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Editorial Material CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN C1 DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT IMMUNOL,PALO ALTO,CA 94304. BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. UNIV GLASGOW,WESTERN INFIRM,DEPT IMMUNOL,GLASGOW G11 6NT,LANARK,SCOTLAND. UNIV PENN,SCH MED,WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104. YALE UNIV,SCH MED,NEW HAVEN,CT 06520. AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV CELL BIOL,CANBERRA,ACT 2601,AUSTRALIA. UNIV PITTSBURGH,DEPT SURG,PITTSBURGH,PA 15261. DEUTSCH RHEUMA FORSCHUNGSZENTRUM BERLIN,D-13353 BERLIN,GERMANY. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL,SAN FRANCISCO,CA 94143. UNIV ALBERTA,DEPT IMMUNOL,EDMONTON,AB T6G 2H7,CANADA. UNIV CHICAGO,DEPT PATHOL,CHICAGO,IL 60637. UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637. WASHINGTON UNIV,SCH MED,DEPT PATHOL,ST LOUIS,MO 63110. UNIV CALIF SAN DIEGO,DEPT BIOL 0063,LA JOLLA,CA 92093. RP Sher, A (reprint author), NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT,NATL INST HLTH,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. NR 16 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 25 EP 33 PG 9 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00006 ER PT S AU Mitchison, NA Sher, A Trinchieri, G Lotze, MT Coffman, RL Lachmann, PJ Ramshaw, IA Liew, FY Rollinghoff, M Kaufmann, SHE Shearer, GM Navikas, V AF Mitchison, NA Sher, A Trinchieri, G Lotze, MT Coffman, RL Lachmann, PJ Ramshaw, IA Liew, FY Rollinghoff, M Kaufmann, SHE Shearer, GM Navikas, V BE Chadwick, D Cardew, G TI General discussion I - Vaccine development SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Discussion CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN C1 NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT,NATL INST HLTH,BETHESDA,MD 20892. UNIV PENN,SCH MED,WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104. UNIV PITTSBURGH,DEPT SURG,PITTSBURGH,PA 15261. DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT IMMUNOL,PALO ALTO,CA 94304. AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV CELL BIOL,CANBERRA,ACT 2601,AUSTRALIA. UNIV GLASGOW,WESTERN INFIRM,DEPT IMMUNOL,GLASGOW G11 6NT,LANARK,SCOTLAND. BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. UNIV ALBERTA,DEPT IMMUNOL,EDMONTON,AB T6G 2H7,CANADA. UNIV ERLANGEN NURNBERG,INST KLIN MIKROBIOL,D-91054 ERLANGEN,GERMANY. MAX PLANCK INST INFECT BIOL,D-10117 BERLIN,GERMANY. NCI,EXPTL IMMUNOL BRANCH,NATL INST HLTH,BETHESDA,MD 20892. HUDDINGE HOSP,DEPT NEUROL,S-14186 HUDDINGE,SWEDEN. MRC CTR,MOLEC IMMUNOPATHOL UNIT,CAMBRIDGE CB2 2QH,ENGLAND. RP Mitchison, NA (reprint author), DEUTSCH RHEUMA FORSCHUNGSZENTRUM BERLIN,NORDUFER 20,D-13353 BERLIN,GERMANY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 35 EP 38 PG 4 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00008 ER PT S AU Fitch, FW Mosmann, TR Abbas, AK Ramshaw, IA Flavell, R Liew, FY Romagnani, S Dutton, RW Mitchison, NA Swain, SL Sher, A McMichael, AJ Kaufmann, SHE Coffman, RL AF Fitch, FW Mosmann, TR Abbas, AK Ramshaw, IA Flavell, R Liew, FY Romagnani, S Dutton, RW Mitchison, NA Swain, SL Sher, A McMichael, AJ Kaufmann, SHE Coffman, RL BE Chadwick, D Cardew, G TI Differentiation of subsets of CD4+ and CD8+ T cells - Discussion SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Discussion CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN ID CLASS-I MHC; MENINGITIS; MOLECULES; CYTOKINES; REJECTION; INVIVO; MODEL C1 UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637. UNIV ALBERTA,DEPT IMMUNOL,EDMONTON,AB T6G 2H7,CANADA. BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. YALE UNIV,SCH MED,NEW HAVEN,CT 06520. AUSTRALIAN NATL UNIV,JOHN CURTIN SCH MED RES,DIV CELL BIOL,CANBERRA,ACT 2601,AUSTRALIA. UNIV GLASGOW,WESTERN INFIRM,DEPT IMMUNOL,GLASGOW G11 6NT,LANARK,SCOTLAND. UNIV FLORENCE,DIV CLIN IMMUNOL & ALLERGY,INST CLIN MED 3,I-50134 FLORENCE,ITALY. UNIV CALIF SAN DIEGO,CTR CANC,DEPT BIOL,LA JOLLA,CA 92093. DEUTSCH RHEUMA FORSCHUNGSZENTRUM BERLIN,D-13353 BERLIN,GERMANY. UNIV CALIF SAN DIEGO,DEPT BIOL 0063,LA JOLLA,CA 92093. NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT,NATL INST HLTH,BETHESDA,MD 20892. JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC IMMUNOL GRP,OXFORD OX3 9DU,ENGLAND. MAX PLANCK INST INFECT BIOL,D-10117 BERLIN,GERMANY. DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT IMMUNOL,PALO ALTO,CA 94304. RP Fitch, FW (reprint author), UNIV CHICAGO,DEPT PATHOL,5841 S MARYLAND AVE,CHICAGO,IL 60637, USA. NR 16 TC 2 Z9 2 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 50 EP 54 PG 5 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00011 ER PT S AU Dutton, RW Sher, A Mosmann, TR Romagnani, S Trinchieri, G Flavell, R Fitch, FW Mitchison, NA Locksley, RM Allen, PM Mason, DW Abbas, AK Swain, SL AF Dutton, RW Sher, A Mosmann, TR Romagnani, S Trinchieri, G Flavell, R Fitch, FW Mitchison, NA Locksley, RM Allen, PM Mason, DW Abbas, AK Swain, SL BE Chadwick, D Cardew, G TI Regulation of T lymphocyte subsets - Discussion SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Editorial Material CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN C1 BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. UNIV CHICAGO,DEPT PATHOL,CHICAGO,IL 60637. UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637. YALE UNIV,SCH MED,NEW HAVEN,CT 06520. WASHINGTON UNIV,SCH MED,DEPT PATHOL,ST LOUIS,MO 63110. UNIV ALBERTA,DEPT IMMUNOL,EDMONTON,AB T6G 2H7,CANADA. DEUTSCH RHEUMA FORSCHUNGSZENTRUM BERLIN,D-13353 BERLIN,GERMANY. UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL,MRC,CELLULAR IMMUNOL UNIT,OXFORD OX1 3RE,ENGLAND. UNIV FLORENCE,INST CLIN MED 3,DIV CLIN IMMUNOL & ALLERGY,I-50134 FLORENCE,ITALY. NIAID,NIH,PARASIT DIS LAB,IMMUNOBIOL SECT,BETHESDA,MD 20892. UNIV CALIF SAN DIEGO,DEPT BIOL 0063,LA JOLLA,CA 92093. RP Dutton, RW (reprint author), UNIV CALIF SAN DIEGO,CTR CANC,DEPT BIOL,9500 GILMAN DR,LA JOLLA,CA 92093, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 80 EP 85 PG 6 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00015 ER PT S AU Sher, A Denkers, EY Gazzinelli, RT AF Sher, A Denkers, EY Gazzinelli, RT BE Chadwick, D Cardew, G TI Induction and regulation of host cell-mediated immunity by Toxoplasma gondii SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Article; Proceedings Paper CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN ID CD8+ T-CELLS; IFN-GAMMA; INTERFERON-GAMMA; RESISTANCE; MICE; ENCEPHALITIS; PARASITE AB Toxoplasma gondii is a highly infectious intracellular parasite which, if left unchecked by the immune system, rapidly overwhelms its intermediate hosts, as illustrated by the pathogenesis of toxoplasmic encephalitis in patients with AIDS. In order to insure both its host's and consequently its own survival simultaneously, T. gondii induces a potent gamma-interferon (IFN-gamma)-dependent cell-mediated immunity early in infection that controls the replication of the protozoan and facilitates transformation into the dormant cyst stage. The protective IFN-gamma is derived from three sources: natural killer cells; and CD4(+) and CD8(+) T lymphocytes, which can partially compensate for each other in knockout mice lacking the appropriate major histocompatibility complex-restricting elements. At least two properties of the parasite appear to be responsible for the early induction of these effector cells. The first is a hydrophobic molecule (or group of related molecules) that triggers interleukin 12 (IL-12), tumour necrosis factor alpha and IL-1 beta synthesis in macrophages. This response can also promote HIV replication in the same cells. The second is a superantigen activity that drives IFN-gamma-producing V beta 5(+) CD8(+) T cells. These potentially lethal responses are later regulated through the triggering of IL-10 and by the induction of anergy in the superantigen-stimulated V beta 5(+) T cell population. RP Sher, A (reprint author), NIAID,NIH,PARASIT DIS LAB,IMMUNOBIOL SECT,BLDG 4,ROOM 126,BETHESDA,MD 20892, USA. NR 22 TC 39 Z9 42 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 95 EP 104 PG 10 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00018 PM 8724832 ER PT S AU Swain, SL Sher, A Kaufmann, SHE Mitchison, NA Locksley, RM Flavell, R Mosmann, TR Abbas, AK Trinchieri, G Lotze, MT Coffman, RL Liew, FY AF Swain, SL Sher, A Kaufmann, SHE Mitchison, NA Locksley, RM Flavell, R Mosmann, TR Abbas, AK Trinchieri, G Lotze, MT Coffman, RL Liew, FY BE Chadwick, D Cardew, G TI Induction and regulation of host cell-mediated immunity by Toxoplasma gondii - Discussion SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Editorial Material CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN ID CD8+ LYMPHOCYTES-T; GAMMA-PRODUCTION; INFECTIONS; CD4+; MICE C1 NIAID,NIH,PARASIT DIS LAB,IMMUNOBIOL SECT,BETHESDA,MD 20892. WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104. UNIV GLASGOW,WESTERN INFIRM,DEPT IMMUNOL,GLASGOW G11 6NT,LANARK,SCOTLAND. UNIV PITTSBURGH,DEPT SURG,PITTSBURGH,PA 15261. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL IMMUNOL,SAN FRANCISCO,CA 94143. DEUTSCH RHEUMA FORSCHUNGSZENTRUM BERLIN,D-13353 BERLIN,GERMANY. UNIV ALBERTA,DEPT IMMUNOL,EDMONTON,AB T6G 2H7,CANADA. BRIGHAM & WOMENS HOSP,DEPT PATHOL,DIV IMMUNOL RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. YALE UNIV,SCH MED,NEW HAVEN,CT 06520. DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT IMMUNOL,PALO ALTO,CA 94304. MAX PLANCK INST INFECT BIOL,D-10117 BERLIN,GERMANY. RP Swain, SL (reprint author), UNIV CALIF SAN DIEGO,DEPT BIOL 0063,9500 GILMAN DR,LA JOLLA,CA 92093, USA. NR 14 TC 3 Z9 3 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 104 EP 109 PG 6 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00019 ER PT S AU Shearer, GM Clerici, M Sarin, A Berzofsky, JA Henkart, PA AF Shearer, GM Clerici, M Sarin, A Berzofsky, JA Henkart, PA BE Chadwick, D Cardew, G TI Cytokines in immune regulation pathogenesis in HIV infection SO T CELL SUBSETS IN INFECTIOUS AND AUTOIMMUNE DISEASES SE CIBA FOUNDATION SYMPOSIA LA English DT Article; Proceedings Paper CT Symposium on T Cell Subsets in Infectious and Autoimmune Diseases CY MAR 07-09, 1995 CL CIBA FDN, LONDON, ENGLAND HO CIBA FDN ID IMMUNODEFICIENCY-VIRUS INFECTION; SEROPOSITIVE INDIVIDUALS; HOMOSEXUAL MEN; T-CELLS; AIDS; INTERLEUKIN-2; RECOGNITION; DEFICIENCY; RESPONSES AB Two hallmarks of immunopathogenesis in the progression of HIV-infected individuals to AIDS are the loss of T helper (Th) cell function in response to antigens and the critical reduction in CD4(+) T cell numbers. It is probable that these two phenomena are related, We observed that: (1) the failure to detect antigen-stimulated Th cell responses in vitro correlates with increased pokeweed mitogen/staphylococcal enterotoxin B (P/S)-stimulated and antigen-stimulated T cell death; and (2) both of these events are similarly modulated by immunoregulatory cytokines. Interleukin 2 (IL-2) and IL-12 (Th1-type cytokines), as well as antibodies to IL-4 and IL-10 (which are Th2-type cytokines) restore in vitro Th cell responses to recall antigens such as influenza virus and HIV envelope synthetic peptides (env). P/S-induced T cell death affects both CD4(+) and CD8(+) T cell subsets, whereas death induced by stimulation with env affects only CD4(+) T cells. In both examples, Th1-type cytokines and antibodies to Th2-type cytokines protect against T cell death. In contrast, IL-4 and IL-10 do not protect against death, and anti-IL-12 antibody can enhance T cell death. Our findings indicate that the loss of Th cell function and increased T cell death seen in vitro are correlated, and that in vivo HIV infection gives rise to inappropriate cytokines resulting in immune dysfunction and immunopathogenesis. C1 NCI,EXPTL IMMUNOL BRANCH,NIH,BETHESDA,MD 20892. NCI,METAB BRANCH,NIH,BETHESDA,MD 20892. RP Shearer, GM (reprint author), UNIV MILAN,CATTEDRA IMMUNOL,MILAN,ITALY. NR 30 TC 12 Z9 12 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI CHICHESTER PA BAFFINS LANE, CHICHESTER, WEST SUSSEX, ENGLAND PO19 1UD SN 0300-5208 BN 0-471-95720-8 J9 CIBA F SYMP PY 1995 VL 195 BP 142 EP 147 PG 6 WC Cell Biology; Immunology; Infectious Diseases; Medicine, General & Internal SC Cell Biology; Immunology; Infectious Diseases; General & Internal Medicine GA BF03F UT WOS:A1995BF03F00025 PM 8724835 ER PT S AU SUFFNESS, M AF SUFFNESS, M BE Georg, GI Chem, TT Ojima, I Vyas, DM TI OVERVIEW OF PACLITAXEL RESEARCH - PROGRESS ON MANY FRONTS SO TAXANE ANTICANCER AGENTS: BASIC SCIENCE AND CURRENT STATUS SE ACS SYMPOSIUM SERIES LA English DT Article; Proceedings Paper CT Symposium on Taxane Anticancer Agents - Basic Science and Current Status, at the 207th National Meeting of the American-Chemical-Society CY MAR 13-17, 1994 CL SAN DIEGO, CA SP AMER CHEM SOC, DIV CHEM HLTH & SAFETY, AMER CHEM SOC, DIV MED CHEM, AMER CHEM SOC, ORGAN CHEM ID PERFORMANCE LIQUID-CHROMATOGRAPHY; ACTIVE TAXOL ANALOGS; TAXUS-BREVIFOLIA; SIDE-CHAIN; BIOLOGICAL EVALUATION; MASS-SPECTROMETRY; PACIFIC YEW; TAXOMYCES-ANDREANAE; PRACTICAL APPROACH; TAXANES AB Great progress has been made in research on taxol production and on future generation taxol drugs since the report of important activity in human ovarian cancer in 1989. Recent results have established that: there is strong long term potential in plantations of either Taxus baccata or several ornamental Taxus cultivars, for either direct production of taxol or production of its precursor, 10-desacetylbaccatin III (10-DAB); plant cell culture, especially utilizing advances in biosynthetic understanding and genetic engineering, is a likely future drug source; semisynthesis of taxol from 10-DAB is well established through major advances in side chain synthesis and coupling reactions; the binding site on microtubules involves both the alpha- and beta-subunits of tubulin, with the N-terminal 31 amino acids of the beta-subunit being critical; effects of single substitutions of substituents at nearly all positions on the taxol molecule have been established; and there is a strong opportunity for new generations of taxol-like drugs through peptidomimetic approaches. RP SUFFNESS, M (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,GRANTS & CONTRACTS OPERAT BRANCH,BETHESDA,MD 20892, USA. NR 95 TC 21 Z9 21 U1 0 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 SIXTEENTH ST NW, WASHINGTON, DC 20036 SN 0097-6156 BN 0-8412-3073-0 J9 ACS SYM SER PY 1995 VL 583 BP 1 EP 17 PG 17 WC Oncology; Chemistry, Medicinal; Chemistry, Multidisciplinary; Chemistry, Organic; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy; Chemistry GA BB96Q UT WOS:A1995BB96Q00001 ER PT B AU HEYES, MP MAJOR, EO SATO, K MARKEY, SM AF HEYES, MP MAJOR, EO SATO, K MARKEY, SM BE Major, EO Levy, JA Schoenberg, D TI Quantification of quinolinic acid metabolism by macrophages and astrocytes SO TECHNICAL ADVANCES IN AIDS RESEARCH IN THE HUMAN NERVOUS SYSTEM LA English DT Proceedings Paper CT NIH Symposium on Technical Advances in AIDS Research in the Human Nervous System CY OCT 04-05, 1993 CL WASHINGTON, DC SP NIH C1 NIMH,CLIN SCI LAB,ANALYT BIOCHEM SECT,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45000-3 PY 1995 BP 317 EP 325 PG 9 WC Infectious Diseases; Medicine, Research & Experimental; Neurosciences SC Infectious Diseases; Research & Experimental Medicine; Neurosciences & Neurology GA BD83E UT WOS:A1995BD83E00023 ER PT B AU LUCK, LA AF LUCK, LA BE Crabb, JW TI F-19 NMR studies of fluorinated sugars binding to the glucose and galactose receptor SO TECHNIQUES IN PROTEIN CHEMISTRY VI SE TECHNIQUES IN PROTEIN CHEMISTRY (ACADEMIC PRESS INC) LA English DT Proceedings Paper CT 8th Symposium of the Protein-Society - Techniques in Protein Chemistry CY JUL 09-13, 1994 CL SAN DIEGO, CA SP PROTEIN SOC RP NIEHS,MOLEC BIOPHYS LAB,POB 12233,RES TRIANGLE PK,NC 27709, USA. NR 0 TC 5 Z9 5 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 BN 0-12-194712-2 J9 TECH PROT CHEM PY 1995 VL 6 BP 487 EP 494 DI 10.1016/S1080-8914(06)80059-1 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BC88B UT WOS:A1995BC88B00056 ER PT B AU DIONNE, RA AF DIONNE, RA BE Sessle, BJ Bryant, PS Dionne, RA TI PHARMACOLOGIC TREATMENTS FOR TEMPOROMANDIBULAR DISORDERS SO TEMPOROMANDIBULAR DISORDERS AND RELATED PAIN CONDITIONS SE PROGRESS IN PAIN RESEARCH AND MANAGEMENT LA English DT Proceedings Paper CT International Workshop on the Temporomandibular Disorders and Related Pain Conditions CY APR, 1994 CL HUNT VALLEY, MD SP NIH, NIDR, NIAMSD, OFF RES WOMENS HLTH, US FDA C1 NIDR,NEUROBIOL & ANESTHESIOL BRANCH,BETHESDA,MD 20892. NR 0 TC 5 Z9 5 U1 0 U2 0 PU INT ASSOC STUDY PAIN (IASP) PRESS PI SEATTLE PA 909 NE 43RD ST, SUITE 304, SEATTLE, WA 98105 BN 0-931092-09-4 J9 PROG PAIN RES MANAG PY 1995 VL 4 BP 363 EP 374 PG 12 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA BD13F UT WOS:A1995BD13F00027 ER PT B AU BRYANT, PS SESSLE, BJ AF BRYANT, PS SESSLE, BJ BE Sessle, BJ Bryant, PS Dionne, RA TI WORKSHOP RECOMMENDATIONS ON RESEARCH NEEDS AND DIRECTIONS SO TEMPOROMANDIBULAR DISORDERS AND RELATED PAIN CONDITIONS SE PROGRESS IN PAIN RESEARCH AND MANAGEMENT LA English DT Proceedings Paper CT International Workshop on the Temporomandibular Disorders and Related Pain Conditions CY APR, 1994 CL HUNT VALLEY, MD SP NIH, NIDR, NIAMSD, OFF RES WOMENS HLTH, US FDA C1 NIDR,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT ASSOC STUDY PAIN (IASP) PRESS PI SEATTLE PA 909 NE 43RD ST, SUITE 304, SEATTLE, WA 98105 BN 0-931092-09-4 J9 PROG PAIN RES MANAG PY 1995 VL 4 BP 467 EP 478 PG 12 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA BD13F UT WOS:A1995BD13F00034 ER PT J AU Saffiotti, U Ahmed, N AF Saffiotti, U Ahmed, N TI Neoplastic transformation by quartz in the BALB/3T3/A31-1-1 cell line and the effects of associated minerals SO TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS LA English DT Article DE crystalline silica; cytotoxicity; hematite; anatase; rutile; chromosomal alterations; gene expression ID CRYSTALLINE SILICA; MAMMALIAN-CELLS; CYTO-TOXICITY; INDUCTION; N-METHYL-N'-NITRO-N-NITROSOGUANIDINE; FUNCTIONALITIES; INHALATION; EXPOSURE; RADICALS; FIBER AB Quartz, the most common form of crystalline silica, was tested quantitatively for neoplastic transformation in the mouse embryo cell line, BALB/3T3/A31-1-1. Five quartz dust samples of respirable size [Min-U-Sil 5 (MQZ); hydrofluoric-acid-etched MQZ (HFMQZ); Chinese standard quartz (CSQZ); DQ12; and F600] all induced significant levels of neoplastic transformation, showing dose-dependent increases in the frequency of morphologically transformed foci at lower tested doses and a plateau level of response at higher doses. The plateau levels reached by the five tested samples did not differ substantially (maximum transformation frequencies per 10(5) cells ranging from 53.2 for MQZ to 28.3 for HFMQZ). F600 had minimal cytotoxicity but transforming activity comparable to the other samples. Cells from all tested transformed foci, when injected s.c. in nude mice, grew as sarcomas. Cytogenetic analysis showed that all tested silica-transformed cell lines had acquired one to five additional marker chromosomes, of types not seen in untreated control lines, indicative of induced chromosomal translocations and amplification. Increased expression of one or more of five genes (p53, myc, H-ras, K-ras, and abl) was observed in several quartz-transformed cell lines. No transforming activity was found for hematite and anatase (both nontoxic), and for rutile (more toxic than MQZ). Combined exposure (1:1 w/w per unit culture area) of each of these dusts with MQZ showed that hematite and anatase inhibited MQZ toxicity as well as transformation, whereas rutile markedly enhanced MQZ toxicity but not MQZ-induced transformation. (C) 1996 Wiley-Liss, Inc. RP Saffiotti, U (reprint author), NCI, LAB EXPT PATHOL, DBS, DEC, NIH, BETHESDA, MD 20892 USA. NR 43 TC 20 Z9 20 U1 1 U2 1 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0270-3211 J9 TERATOGEN CARCIN MUT JI Teratogenesis Carcinog. Mutagen. PY 1995 VL 15 IS 6 BP 339 EP 356 DI 10.1002/tcm.1770150609 PG 18 WC Oncology; Genetics & Heredity; Toxicology SC Oncology; Genetics & Heredity; Toxicology GA UH259 UT WOS:A1995UH25900008 PM 8732883 ER PT J AU RONGWEN, J AKKAYA, MS BHAGWAT, AA LAVI, U CREGAN, PB AF RONGWEN, J AKKAYA, MS BHAGWAT, AA LAVI, U CREGAN, PB TI THE USE OF MICROSATELLITE DNA MARKERS FOR SOYBEAN GENOTYPE IDENTIFICATION SO THEORETICAL AND APPLIED GENETICS LA English DT Article DE SIMPLE SEQUENCE REPEATS; DNA FINGER PRINTING; GENE DIVERSITY; PLANT VARIETY PROTECTION; DNA MARKERS ID LENGTH POLYMORPHISMS; LINKAGE MAP; DIVERSITY AB Conventional morphological and pigementation traits, as well as disease resistance, have been used to distinguish the uniqueness of new soybean cultivars for purposes of plant variety protection. With increasing numbers of cultivars and a finite number of conventional characters, it has become apparent that such traits will not suffice to establish uniqueness. The objective of this work was to provide an initial evaluation of microsatellite or simple-sequence-repeat (SSR) DNA markers to develop unique DNA profiles of soybean genotypes. Microsatellites are DNA sequences such as (AT)(n)/(TA)(n) and (ATT)(n)/(TAA)(n) that are composed of tandemly repeated 2-5-basepair DNA core sequences. The DNA sequences flanking microsatellites are generally conserved allowing the selection of polymerase chain reaction (PCR) primers that will amplify the intervening SSR. Variation in the number of tandem repeats, ''n'', results in PCR product length differences. The SSR alleles present at three (AT)(n)/(TA)(n) and four (ATT)(n)/(TAA)(n) loci were determined in each of 96 diverse soybean genotypes. Between 11 and 26 alleles were found at each of the seven loci. Only two genotypes had identical SSR allelic profiles and these had very similar pedigrees. The gene diversity for the seven markers averaged 0.87 for all 96 genotypes and 0.74 for a subset of 26 North American cultivars. These are much higher than soybean gene diversity values obtained using RELP markers, and are similar to the average values obtained for human microsatellite markers, SSR markers provide an excellent complement to the conventional markers that are currently used to characterize soybean genotypes. C1 USDA ARS,SOYBEAN & ALFALFA RES LAB,BELTSVILLE,MD 20705. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. UNIV MARYLAND,DEPT AGRON,COLLEGE PK,MD 20742. AGR RES ORG,VOLCANI CTR,INST HORT,IL-50250 BET DAGAN,ISRAEL. NR 17 TC 223 Z9 285 U1 2 U2 14 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JAN PY 1995 VL 90 IS 1 BP 43 EP 48 PG 6 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA QF110 UT WOS:A1995QF11000006 PM 24173782 ER PT J AU SCHIEWE, MC HOLLIFIELD, VM KASBOHM, LA SCHMIDT, PM AF SCHIEWE, MC HOLLIFIELD, VM KASBOHM, LA SCHMIDT, PM TI EMBRYO IMPORTATION AND CRYOBANKING STRATEGIES FOR LABORATORY-ANIMALS AND WILDLIFE SPECIES SO THERIOGENOLOGY LA English DT Article; Proceedings Paper CT Annual Conference of the International-Embryo-Transfer-Society CY JAN 08-10, 1995 CL CALGARY, CANADA SP INT EMBRYO TRANSFER SOC DE EMBRYO CRYOPRESERVATION; IMPORTATION; REGULATION; LABORATORY ANIMAL; WILDLIFE ID INFECTIOUS-DISEASE CONTROL; MOUSE EMBRYOS; VIRUS; MICE; REINTRODUCTION; SURVEILLANCE; TRANSPORT AB The transportation of embryos obtained from animal models, endangered species and nondomestic farmed animals (e.g., deer) can reduce/eliminate the need for;shipping postnatal animals and thus has gained the interest of the biomedical and conservation fields. Efficient movement of germ plasm worldwide requires established cryobanks. Embryo cryopreservation has become a routinely successful technology for many species and efforts to develop usable cryobanks for many target species are ongoing. Recommended regulations for the movement of embryos from nontraditional (i.e. other than domestic livestock) species are nonexistent. Efforts are underway to establish domestic and international handling guidelines and to recommend suitable quarantine conditions to facilitate embryo importation. Further basic research on specific zona pellucida-pathogen interactions is encouraged to support embryo movement efforts. C1 UNIV CALIF IRVINE, DEPT OBSTET & GYNECOL, IRVINE, CA 92717 USA. NIH, NATL CTR RES RESOURCES, BETHESDA, MD 20892 USA. NR 42 TC 7 Z9 7 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X J9 THERIOGENOLOGY JI Theriogenology PD JAN PY 1995 VL 43 IS 1 BP 97 EP 104 DI 10.1016/0093-691X(94)00012-J PG 8 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA PY150 UT WOS:A1995PY15000012 ER PT B AU Schuh, LM Henningfield, JE AF Schuh, LM Henningfield, JE BE Slama, K TI Nicotine replacement treatment and public health interventions Toward a marriage of two approaches SO TOBACCO AND HEALTH LA English DT Proceedings Paper CT 9th World Conference on Tobacco and Health CY OCT 10-14, 1994 CL PARIS, FRANCE SP WHO, Int Union Against Canc, Int Union Against Tuberculosis & Lung Dis, Int Soc & Federat Cardiol, Int Union Hlth Promot & Educ, Int Org Consumers Unions C1 NIDA,ADDICT RES CTR,CLIN PHARMACOL BRANCH,BALTIMORE,MD 21224. NR 0 TC 1 Z9 1 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45111-5 PY 1995 BP 419 EP 424 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA BE95N UT WOS:A1995BE95N00082 ER PT J AU STEFANSKI, SA GREENWELL, A MERRICK, BA BROWN, TT REYNOLDS, SH AF STEFANSKI, SA GREENWELL, A MERRICK, BA BROWN, TT REYNOLDS, SH TI PROLIFERATING CELL NUCLEAR ANTIGEN STAINING OF FISCHER-344/N RAT SPLEENS AFFECTED BY LARGE GRANULAR LYMPHOCYTE LEUKEMIA SO TOXICOLOGIC PATHOLOGY LA English DT Article DE IMMUNOHISTOCHEMISTRY; LEUKEMIA; LEUKEMIA STAGING; CELL PROLIFERATION AB Large granular lymphocyte (LGL) leukemia commonly occurs in the Fischer-344/N rat, The high spontaneous incidence complicates the interpretation of results from chronic carcinogenicity studies that use this rat strain. As a result, a comprehensive characterization of LGL leukemia is necessary to help understand the leukemogenic process and the applicability of staging for assessing the progression of this disease. In the current study, the proliferation rate of LGL leukemia cells from untreated control Fischer-344 (F-344) rats in 3 stages of leukemia compared to nonleukemic age-matched rats was determined by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). In histologic sections of spleen from aged F-344/N rats affected by LGL leukemia, there was a significant increase of both PCNA labeling and mitotic indices that was most advanced in the spleen of rats with more severe LGL leukemia. These results support biological significance for the morphologic staging system currently in use. C1 NIEHS,EXPTL PATHOL LAB,RES TRIANGLE PK,NC 27709. NR 0 TC 3 Z9 3 U1 0 U2 0 PU SOC TOXICOLOGIC PATHOLOGISTS PI LAWRENCE PA 1041 NEW HAMPSHIRE ST PO BOX 368, LAWRENCE, KS 66044 SN 0192-6233 J9 TOXICOL PATHOL JI Toxicol. Pathol. PD JAN-FEB PY 1995 VL 23 IS 1 BP 1 EP 6 PG 6 WC Pathology; Toxicology SC Pathology; Toxicology GA QL667 UT WOS:A1995QL66700001 PM 7770695 ER PT J AU BUCHER, JR MORGAN, DL ADKINS, B TRAVLOS, GS DAVIS, BJ MORRIS, R ELWELL, MR AF BUCHER, JR MORGAN, DL ADKINS, B TRAVLOS, GS DAVIS, BJ MORRIS, R ELWELL, MR TI EARLY CHANGES IN SEX-HORMONES ARE NOT EVIDENT IN MICE EXPOSED TO THE UTERINE CARCINOGENS CHLOROETHANE OR BROMOETHANE SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID VAGINAL CYTOLOGY; CANCER AB Chloroethane and bromoethane have been shown to cause a marked uterine tumor response in B6C3F(1) mice exposed for 2 years. These chemicals are nearly unique in this regard among the nearly 400 chemicals studied by the National Toxicology Program, and the reasons for this carcinogenic activity are unclear. The possible relationship of changes in blood concentrations of sex hormones to this response was evaluated by examining the estrous cycle of mice prior to and during a 21-day exposure to concentrations of the haloethanes which resulted in the tumorigenic response in the 2-year studies. Serum concentrations of estradiol and progesterone were determined at the termination of the exposures and compared to exposure group and stage of the estrous cycle. No consistent patterns of change were found in estrous cyclicity or in blood concentrations of sex hormones. Thus, the findings suggest that early changes in circulating sex hormones are not important contributing factors in the uterine neoplasia caused by these chemicals. (C) 1995 Academic Press, Inc. C1 NIEHS,RES TRIANGLE PK,NC 27709. MANTECH ENVIRONM TECHNOL INC,RES TRIANGLE PK,NC 27709. ANALYT SCI INC,RES TRIANGLE PK,NC 27709. NR 14 TC 12 Z9 12 U1 1 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD JAN PY 1995 VL 130 IS 1 BP 169 EP 173 DI 10.1006/taap.1995.1022 PG 5 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QC928 UT WOS:A1995QC92800022 PM 7839365 ER PT J AU DREYER, G COUTINHO, A MIRANDA, D NOROES, J RIZZO, JA GALDINO, E ROCHA, A MEDEIROS, Z ANDRADE, LD SANTOS, A FIGUEREDOSILVA, J OTTESEN, EA AF DREYER, G COUTINHO, A MIRANDA, D NOROES, J RIZZO, JA GALDINO, E ROCHA, A MEDEIROS, Z ANDRADE, LD SANTOS, A FIGUEREDOSILVA, J OTTESEN, EA TI TREATMENT OF BANCROFTIAN FILARIASIS IN RECIFE, BRAZIL - A 2-YEAR COMPARATIVE-STUDY OF THE EFFICACY OF SINGLE TREATMENTS WITH IVERMECTIN OR DIETHYLCARBAMAZINE SO TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE LA English DT Article DE FILARIASIS; WUCHERERIA BANCROFTI; IVERMECTIN; DIETHYLCARBAMAZINE; BRAZIL ID HIGH-DOSE IVERMECTIN; MICROFILAREMIA AB The effectiveness of single oral doses of ivermectin (200 or 400 mu g/kg) and diethylcarbamazine (DEC, 6 mg/kg), preceded 4 d earlier by either placebo or very small doses of these drugs, was compared, over a 2-year period, in a double-blind trial in 67 microfilaraemic Brazilian men with bancroftian filariasis. Regimens containing ivermectin alone decreased the number of microfilariae significantly faster and more effectively for the first month after treatment than regimens containing DEC alone, but the latter were significantly more effective throughout the second year after treatment (1.7-8.2% of pretreatment levels with DEC vs. 12.6-30.8% with ivermectin during that period); the higher ivermectin dose showed a tendency towards more effectiveness than the lower dose. Most effective was the combination of ivermectin (20 mu g/kg) followed 4 d later by DEC (6 mg/kg), with reduction of microfilaraemia to 2.4% of pretreatment levels at 2 years. Adverse reactions were well tolerated with all regimens, the reactions being significantly more generalized (i.e., fever) following ivermectin and localized (i.e., scrotal inflammatory nodules around dying adult worms) following DEC. Further trials of single-dose combination therapy vs. single high doses of ivermectin or DEC should determine the ideal regimen for treatment and control of bancroftian filariasis. C1 UNIV FED PERNAMBUCO,HOSP CLIN,RECIFE,PE,BRAZIL. UNIV FED PERNAMBUCO,IMMUNOL KEISO ASAMI LAB,RECIFE,PE,BRAZIL. NIH,BETHESDA,MD 20892. RP DREYER, G (reprint author), FIOCRUZ MS,CTR PESQUISAS AGGEU MAGALHAES,CAMPUS UNIV FED PERNAMBUCO,AV MORALES REGO S-N,BR-50730 RECIFE,PE,BRAZIL. NR 18 TC 49 Z9 51 U1 0 U2 1 PU ROYAL SOC TROPICAL MEDICINE PI LONDON PA MANSON HOUSE 26 PORTLAND PLACE, LONDON, ENGLAND W1N 4EY SN 0035-9203 J9 T ROY SOC TROP MED H JI Trans. Roy. Soc. Trop. Med. Hyg. PD JAN-FEB PY 1995 VL 89 IS 1 BP 98 EP 102 DI 10.1016/0035-9203(95)90674-6 PG 5 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA QY300 UT WOS:A1995QY30000028 PM 7747322 ER PT J AU MOSLEY, JW STEVENS, CE AACH, RD HOLLINGER, FB MIMMS, LT SOLOMON, LR BARBOSA, LH NEMO, GJ AF MOSLEY, JW STEVENS, CE AACH, RD HOLLINGER, FB MIMMS, LT SOLOMON, LR BARBOSA, LH NEMO, GJ TI DONOR SCREENING FOR ANTIBODY TO HEPATITIS-B CORE ANTIGEN AND HEPATITIS-B VIRUS-INFECTION IN TRANSFUSION RECIPIENTS SO TRANSFUSION LA English DT Article ID POLYMERASE CHAIN-REACTION; POSTTRANSFUSION HEPATITIS; ALANINE AMINOTRANSFERASE; BLOOD-DONORS; C VIRUS; SURFACE-ANTIGEN; NON-A; TRANSMITTED VIRUSES; ENZYME-IMMUNOASSAY; OCCULT INFECTION AB Background: Testing for antibody to hepatitis B core antigen (anti-HBc) as a surrogate for hepatitis C viremia is no longer needed for blood donor screening. Currently, the important question is how much its use supplements hepatitis B surface antigen (HBsAg) donor screening in preventing transfusion-transmitted hepatitis B virus (HBV) infection. Study Design and Methods: In a study conducted ih the 1970s. 64 blood donors were associated with 15 cases of HBV (1.0%) in 1533 transfusion recipients. Sera from 61 donors at donation and 29 follow-up visits were available for present-day assays for HBsAg, HBV DNA, anti-HBc, and antibody to HBsAg (anti-HBs). Results: HBsAg was found in four previously negative blood donors; HBV DNA was limited to three of these four. Anti-HBc was detected In six HBsAg-negative donors. Two other donors were negative in all assays at donation, but positive for anti-HBc and anti-HBs 2 to 4 months later. The remaining donors were negative for all HBV markers which left five recipient cases unexplained: No HBV transmission was observed when anti-HBs sample-to-negative control values were greater than or equal to 10. Conclusion: Some 33 to 50 percent of cases of hepatitis B that could be transmitted by transfusion of blood from HBsAg-negative donors are prevented by anti-HBc screening. Anti-HBc-positive donors unequivocally positive for anti-HBs should be considered noninfectious for HBV and should be allowed td donate. Anti HBc screening of paid plasmapheresis donors, supplemented by anti-HBs testing, would reduce the amount of HBV to be processed by virus inactivation and increase the content of anti-HBs in plasma pools. C1 UNIV SO CALIF,TRANSFUS SAFETY STUDY,LOS ANGELES,CA 90032. NEW YORK BLOOD CTR,LINDSLEY F KIMBALL RES INST,NEW YORK,NY 10021. CASE WESTERN RESERVE UNIV,MT SINAI MED CTR,DEPT MED,CLEVELAND,OH. BAYLOR COLL MED,HOUSTON,TX. ABBOTT LABS,N CHICAGO,IL. NHLBI,TRANSFUS MED BRANCH,BETHESDA,MD. NHLBI,TRANSFUS MED SCI RES GRP,BETHESDA,MD. ABBOTT LABS,ABBOTT PK,IL. RP MOSLEY, JW (reprint author), UNIV SO CALIF,DEPT MED,EDMONDSON BLDG,ROOM 108,1840 N SOTO ST,LOS ANGELES,CA 90032, USA. FU NHLBI NIH HHS [N01-HB-42972] NR 52 TC 94 Z9 99 U1 0 U2 3 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD JAN PY 1995 VL 35 IS 1 BP 5 EP 12 DI 10.1046/j.1537-2995.1995.35195090661.x PG 8 WC Hematology SC Hematology GA PY552 UT WOS:A1995PY55200003 PM 7998069 ER PT J AU KAO, KJ MICKEL, M BRAINE, HG DAVIS, K ENRIGHT, H GERNSHEIMER, T GILLESPIE, MJ KICKLER, TS LEE, EJ MCCULLOUGH, JJ MCFARLAND, JG NEMO, GJ NOYES, WD SCHIFFER, CA SELL, K SLICHTER, SJ WOODSON, RD AF KAO, KJ MICKEL, M BRAINE, HG DAVIS, K ENRIGHT, H GERNSHEIMER, T GILLESPIE, MJ KICKLER, TS LEE, EJ MCCULLOUGH, JJ MCFARLAND, JG NEMO, GJ NOYES, WD SCHIFFER, CA SELL, K SLICHTER, SJ WOODSON, RD TI WHITE CELL-REDUCTION IN PLATELET CONCENTRATES AND PACKED RED-CELLS BY FILTRATION - A MULTICENTER CLINICAL-TRIAL SO TRANSFUSION LA English DT Article ID LEUKOCYTE-POOR; ALLOIMMUNIZATION; TRANSFUSION; IRRADIATION; ANTIGENS; PREVENTION; DEPLETION; FILTERS; DONORS AB Background: Most previous studies on white cell (WBC) reduction by filtration have been small-scale studies conducted under tightly controlled laboratory conditions. Their results would be the ideal, rather than what might be expected during routine operation. Study Design and Methods: To obtain information on routine filtration of blood components, data have been collected from a large-scale ongoing, multicenter clinical trial designed to determine the effectiveness of WBC reduction in or ultraviolet B radiation of platelet concentrates before transfusion in preventing platelet alloimmunization and platelet transfusion refractoriness. The WBC content of blood components both before and after filtration was determined by automated cell counters and a manual propidium iodide-staining method, respectively, Platelet and red cell losses during filtration were measured. Results: The average platelet losses after filtration were 24 +/- 15 percent and 20 +/- 9 percent for apheresis platelets and pooled platelets, respectively The frequencies at which filtered platelet concentrates contained high levels of residual WBCs (>5 x 10(6)) were 7 percent and 5 percent for apheresis platelets and pooled Platelets, respectively. Further analysis of the platelet filtration data showed that greater numbers of total initial WBCs in the pooled platelets were associated with increased percentages of filtration failure (>5 x 10(6) residual WBCs). For packed red cells, the average losses during filtration were 23 +/- 4 percent and 15 +/- 3 percent for CPDA-1 units and Adsol units, respectively. The frequencies at which filtered red cells contained >5 x 10(6) residual WBCs were 2.7 percent for one type of filter and 0.3 percent for another type of filter. Conclusion: There were significant losses of platelets during filtration, which could add to the costs. of WBC reduction and lead to possible increases in donor exposures. Filtration failures still occurred, despite careful observation of the Standard filtration procedures. The number of total WBCs in pooled platelets before filtration has been identified as an important factor in determining the success of WBC reduction. C1 JOHNS HOPKINS UNIV,DEPT MED,BALTIMORE,MD. UNIV WASHINGTON,DEPT BIOSTAT,SEATTLE,WA 98195. PUGET SOUND BLOOD CTR,PLATELET ANTOBODY LAB,SEATTLE,WA. UNIV MARYLAND,DEPT MED,COLLEGE PK,MD 20742. BLOOD CTR SE WISCONSIN INC,MILWAUKEE,WI. NHLBI,BLOOD RESOURCES BRANCH,BETHESDA,MD. EMORY UNIV,SCH MED,DEPT PATHOL,ATLANTA,GA. PUGET SOUND BLOOD CTR,DIV RES & EDUC,SEATTLE,WA. UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV MINNESOTA,DEPT MED,MINNEAPOLIS,MN. UNIV MINNESOTA,DEPT LAB MED & PATHOL,MINNEAPOLIS,MN. RP KAO, KJ (reprint author), UNIV FLORIDA,DEPT PATHOL,GAINESVILLE,FL 32610, USA. FU NHLBI NIH HHS [U01 HL-42802, U01 HL-42799, U01 HL-42810] NR 22 TC 69 Z9 69 U1 0 U2 1 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD JAN PY 1995 VL 35 IS 1 BP 13 EP 19 DI 10.1046/j.1537-2995.1995.35195090653.x PG 7 WC Hematology SC Hematology GA PY552 UT WOS:A1995PY55200004 PM 7998062 ER PT J AU Hagen, SJ Hofrichter, HJ Bunn, HF Eaton, WA AF Hagen, SJ Hofrichter, HJ Bunn, HF Eaton, WA TI Comments on the physics and chemistry of trehalose as a storage medium for hemoglobin-based blood substitutes: ''From Kramers theory to the battlefield'' SO TRANSFUSION CLINIQUE ET BIOLOGIQUE LA English DT Article ID LIPOSOME-ENCAPSULATED HEMOGLOBIN; DYNAMICS; PRESERVATION; MYOGLOBIN; STABILITY AB A glass of the naturally-occurring sugar trehalose may be a suitable medium for the storage of hemoglobin-based blood substitutes. Trehalose has many or possibly all of the properties required for this purpose, including solubilization of hemoglobin to a very-high concentration, lack of toxicity, slowing of oxidation to the non;oxygen binding methemoglobin, stability at room temperature and above, and ease of transport. It should also be possible to prepare hemoglobin extremely rapidly for injection into the circulation in situations where blood replacement is required immediately, as in a domestic emergency room or on the battlefield. These practical considerations are briefly discussed, as well as the theoretical reasons for slowing of chemical reactions in the glassy state. C1 NIDDKD,CHEM PHYS LAB,BETHESDA,MD 20892. HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DIV HEMATOL ONCOL,BOSTON,MA 02115. RI Hagen, Stephen/E-9737-2015 OI Hagen, Stephen/0000-0002-3373-5033 NR 26 TC 7 Z9 7 U1 1 U2 1 PU ARNETTE-BLACKWELL S A PI PARIS PA 224, BD ST GERMAIN, 75007 PARIS, FRANCE SN 1246-7820 J9 TRANSFUS CLIN BIOL JI Transfu. Clin. Biol. PY 1995 VL 2 IS 6 BP 423 EP 426 DI 10.1016/S1246-7820(05)80066-7 PG 4 WC Hematology; Immunology SC Hematology; Immunology GA TR223 UT WOS:A1995TR22300002 PM 8646336 ER PT J AU MCKNIGHT, RA WALL, RJ HENNIGHAUSEN, L AF MCKNIGHT, RA WALL, RJ HENNIGHAUSEN, L TI EXPRESSION OF GENOMIC AND CDNA TRANSGENES AFTER COINTEGRATION IN TRANSGENIC MICE SO TRANSGENIC RESEARCH LA English DT Article ID GENE-EXPRESSION; EFFICIENCY; SEQUENCES; INTRONS; ENHANCE; RNA AB In general, genomic transgenes are expressed efficiently in mice, while their cDNA-based transgenes are frequently silent. Clark et al (1992) have shown that silent cDNA transgenes under the control of the sheep beta-lactoglobulin promoter can be activated after co-injecting them with a genomic sheep beta-lactoglobulin transgene. We have tested the general utility of this concept using mouse whey acidic protein (WAP) transgenes. Here we show that WAP cDNA transgenes are virtually silent in transgenic mice. In contrast, WAP transgenes containing all introns are expressed in approximately 50% of the lines at levels ranging from 1% to more than 100% of the endogenous RNA (McKnight et al., 1992). When a WAP-genomic transgene was co-injected with a WAP-cDNA, basal activation of the cDNA was possible. However, the activity of the WAP-cDNA transgene did not exceed 1% of the WAP-genomic transgene. This suggests that a permissive integration site capable of supporting basal level transcription can be established, but further events are required for full activation of the transgene. C1 USDA ARS,BELTSVILLE,MD 20725. NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892. RP MCKNIGHT, RA (reprint author), UNIV PITTSBURGH,CHILDRENS HOSP PITTSBURGH,SCH MED,RANGOS RES CTR,DEPT PEDIAT,DIV INNUNOGENET,PITTSBURGH,PA 15213, USA. NR 15 TC 16 Z9 16 U1 1 U2 2 PU CHAPMAN HALL LTD PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN SN 0962-8819 J9 TRANSGENIC RES JI Transgenic Res. PD JAN PY 1995 VL 4 IS 1 BP 39 EP 43 DI 10.1007/BF01976500 PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA QB428 UT WOS:A1995QB42800007 PM 7881461 ER PT J AU RUDD, KE SOFIA, HJ KOONIN, EV PLUNKETT, G LAZAR, S ROUVIERE, PE AF RUDD, KE SOFIA, HJ KOONIN, EV PLUNKETT, G LAZAR, S ROUVIERE, PE TI A NEW FAMILY OF PEPTIDYL-PROLYL ISOMERASES SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Article ID CYCLOPHILIN HOMOLOG NINAA; CIS-TRANS-ISOMERASE; NITROGENASE IRON PROTEIN; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; CATALYSIS; IMMUNOPHILINS; PRSA; LIPOPROTEIN; MUTAGENESIS C1 UNIV WISCONSIN,GENET LAB,MADISON,WI 53706. HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115. UNIV CALIF SAN FRANCISCO,SCH MED,DEPT STOMATOL,SAN FRANCISCO,CA 94143. RP RUDD, KE (reprint author), NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894, USA. RI Lazar, Sara/G-3809-2012; Plunkett III, Guy/B-8058-2009 OI Lazar, Sara/0000-0003-1126-8363; Plunkett III, Guy/0000-0003-1422-469X NR 34 TC 78 Z9 78 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD JAN PY 1995 VL 20 IS 1 BP 12 EP 14 DI 10.1016/S0968-0004(00)88940-9 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QC774 UT WOS:A1995QC77400004 PM 7878731 ER PT J AU KOONIN, EV VANDERVIES, SM AF KOONIN, EV VANDERVIES, SM TI CONSERVED SEQUENCE MOTIFS IN BACTERIAL AND BACTERIOPHAGE CHAPERONINS SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Article ID ALIGNMENT C1 UNIV GENEVA,DEPT BIOCHIM MED,CH-1211 GENEVA 4,SWITZERLAND. RP KOONIN, EV (reprint author), NATL LIB MED,NATL CTR BIOTECHNOL INFORMAT,BETHESDA,MD 20894, USA. NR 8 TC 25 Z9 26 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD JAN PY 1995 VL 20 IS 1 BP 14 EP 15 DI 10.1016/S0968-0004(00)88941-0 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QC774 UT WOS:A1995QC77400005 PM 7878732 ER PT J AU HENGEN, PN AF HENGEN, PN TI METHODS AND REAGENTS - WAYWARD PCR PRIMERS SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Review AB Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the failure of PCR primers that have been kept a long time to amplify DNA. For details on how to partake in the newsgroup, see the accompanying box. RP HENGEN, PN (reprint author), NCI,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702, USA. NR 5 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD JAN PY 1995 VL 20 IS 1 BP 42 EP 44 DI 10.1016/S0968-0004(00)88950-1 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QC774 UT WOS:A1995QC77400019 PM 7878744 ER PT J AU ALBIN, RL TAGLE, DA AF ALBIN, RL TAGLE, DA TI GENETICS AND MOLECULAR-BIOLOGY OF HUNTINGTONS-DISEASE SO TRENDS IN NEUROSCIENCES LA English DT Article ID CAG REPEAT LENGTH; TRINUCLEOTIDE REPEAT; STRIATAL NEURONS; INSTABILITY; MUTATIONS; EXPRESSION; EXPANSION; MECHANISM; RECEPTOR; TISSUES AB In 1993, the genetic abnormality responsible for Huntington's disease was identified as a trinucleotide-repeat expansion in a novel gene. Much has been Teamed about the molecular genetics of Huntington's disease and the possible effects of the trinucleotide expansion in the development of this disease and other neurological disorders. The Huntington's disease locus is widely expressed throughout the brain and in many non-neural tissues. Current speculation about the pathogenesis of neuronal death concentrates on a 'gain of function' effect in which the abnormal protein has acquired a new and lethal property. Future research will define the normal function of the Huntington's disease locus, test hypotheses regarding the putative gain of function, and explore the factors that determine neuronal susceptibility to the effects of the abnormal allele. C1 NATL CTR HUMAN GENOME RES, GENE TRANSFER LAB, BETHESDA, MD 20892 USA. RP UNIV MICHIGAN, DEPT NEUROL, ANN ARBOR, MI 48109 USA. FU NIA NIH HHS [AG08671]; NINDS NIH HHS [NS19613] NR 54 TC 110 Z9 110 U1 3 U2 14 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0166-2236 J9 TRENDS NEUROSCI JI Trends Neurosci. PD JAN PY 1995 VL 18 IS 1 BP 11 EP 14 DI 10.1016/0166-2236(95)93943-R PG 4 WC Neurosciences SC Neurosciences & Neurology GA QB152 UT WOS:A1995QB15200005 PM 7535483 ER PT J AU RAUSCHECKER, JP AF RAUSCHECKER, JP TI COMPENSATORY PLASTICITY AND SENSORY SUBSTITUTION IN THE CEREBRAL-CORTEX SO TRENDS IN NEUROSCIENCES LA English DT Review ID PRIMARY AUDITORY-CORTEX; BLIND MOLE RAT; SUPERIOR COLLICULUS; VISUAL-CORTEX; HEBB SYNAPSES; ADULT CATS; MOTOR MAPS; ORGANIZATION; SPACE; REPRESENTATION AB Cats deprived visually from birth show few overt impairments in their natural behavior. Therefore, they seem well suited as an animal model for the study of compensatory plasticity after early vision loss. It can be demonstrated that binocularly deprived cats show improved abilities of auditory localization, and at least equal tactile behavior compared to normal controls. Within the anterior ectosylvian cortex of binocularly deprived cats, where different sensory modalities come together, the anterior ectosylvian visual area is completely taken over by auditory and somatosensory inputs. Furthermore, the auditory spatial tuning of single units in this cortical region is sharpened significantly as a result of visual deprivation. Somatosensory compensation for early loss of vision can be demonstrated by a hypertrophy of the facial vibrissae, and a corresponding expansion of their central representation in the somatosensory cortex of binocularly deprived animals. The compensatory changes in the cortex can be explained by a reorganization of sensory representations under the guidance of sensorimotor feedback rather than by instruction through an extraneous 'supervisory' signal. These processes might form the neural basis of sensory substitution in blind humans. RP RAUSCHECKER, JP (reprint author), NIMH,NEUROPSYCHOL LAB,BLDG 49,RM 1880,BETHESDA,MD 20892, USA. RI Rauschecker, Josef/A-4120-2013 NR 90 TC 333 Z9 341 U1 1 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB SN 0166-2236 J9 TRENDS NEUROSCI JI Trends Neurosci. PD JAN PY 1995 VL 18 IS 1 BP 36 EP 43 DI 10.1016/0166-2236(95)93948-W PG 8 WC Neurosciences SC Neurosciences & Neurology GA QB152 UT WOS:A1995QB15200012 PM 7535489 ER PT J AU THOMPSON, IM KRAMER, BS AF THOMPSON, IM KRAMER, BS TI EFFECT OF AGE AND RACE ON THE SURVIVAL OF MEN WITH PROSTATE-CANCER IN THE METROPOLITAN DETROIT TRICOUNTY AREA 1973 TO 1987 - COMMENT SO UROLOGY LA English DT Editorial Material ID CARCINOMA; STAGE; RISK C1 NCI,DIV CANC PREVENT & CONTROL,BETHESDA,MD 20892. RP THOMPSON, IM (reprint author), BROOKE ARMY MED CTR,UROL SERV,SAN ANTONIO,TX, USA. NR 13 TC 0 Z9 0 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD JAN PY 1995 VL 45 IS 1 BP 101 EP 102 DI 10.1016/S0090-4295(95)97042-8 PG 2 WC Urology & Nephrology SC Urology & Nephrology GA QB046 UT WOS:A1995QB04600020 ER PT S AU BERZOFSKY, JA AHLERS, JD ALEXANDERMILLER, M TSUKUI, T PENDLETON, CD DUNLOP, N CARBONE, DP NARA, PL AF BERZOFSKY, JA AHLERS, JD ALEXANDERMILLER, M TSUKUI, T PENDLETON, CD DUNLOP, N CARBONE, DP NARA, PL BE Chanock, RM Brown, F Ginsberg, HS Norrby, E TI Developing synthetic peptide vaccines for HIV-1 SO VACCINES 95 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES SE VACCINES (COLD SPRING HARBOR LABORATORY PRESS) LA English DT Proceedings Paper CT 12th Annual Meeting on Molecular Approaches to the Control of Infectious Diseases CY OCT, 1994 CL COLD SPRING HARBOR, NY SP Alafi Capital Co, Amer Cyanamid Co, Amgen Inc, Becton Dickinson & Co, Biogen, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chugai Pharm Co Ltd, Ciba Geigy Corp, Diagnost Prod Corp, Du Pont Merck Pharm Co, Forest Labs Inc, Genentech Inc, Glaxco, Hoffman La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Mitsubishi Kasei Inst Life Sci, Monsanto Co, New England BioLabs Inc, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Pfizer Inc, Res Genet, Sandoz Res Inst, Schering Plough Corp, SmithKline Beecham Pharm, Sterling Winthrop Inc, Sumitomo Pharm Co Ltd, Tekeda Chem Ind Ltd, Toyobo Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC C1 NCI,METAB BRANCH,MOLEC IMMUNOGENET & VACCINE RES SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU COLD SPRING HARBOR LABORATORY PRESS PI PLAINVIEW PA 10 SKYLINE DRIVE, PLAINVIEW, NY 11803-2500 SN 0899-4056 BN 0-87969-467-X J9 VACCINES PY 1995 BP 135 EP 142 PG 8 WC Immunology; Infectious Diseases; Medicine, Research & Experimental SC Immunology; Infectious Diseases; Research & Experimental Medicine GA BD72G UT WOS:A1995BD72G00022 ER PT S AU HIRSCH, VM GOLDSTEIN, S CHANOCK, R ELKINS, WR SUTTER, G MOSS, B SISLER, J LIFSON, J FUERST, T AF HIRSCH, VM GOLDSTEIN, S CHANOCK, R ELKINS, WR SUTTER, G MOSS, B SISLER, J LIFSON, J FUERST, T BE Chanock, RM Brown, F Ginsberg, HS Norrby, E TI Limited virus replication following SIV challenge of macaques immunized with attenuated MVA vaccinia expressing SIVsm env and gag-pol SO VACCINES 95 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES SE VACCINES (COLD SPRING HARBOR LABORATORY PRESS) LA English DT Proceedings Paper CT 12th Annual Meeting on Molecular Approaches to the Control of Infectious Diseases CY OCT, 1994 CL COLD SPRING HARBOR, NY SP Alafi Capital Co, Amer Cyanamid Co, Amgen Inc, Becton Dickinson & Co, Biogen, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chugai Pharm Co Ltd, Ciba Geigy Corp, Diagnost Prod Corp, Du Pont Merck Pharm Co, Forest Labs Inc, Genentech Inc, Glaxco, Hoffman La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Mitsubishi Kasei Inst Life Sci, Monsanto Co, New England BioLabs Inc, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Pfizer Inc, Res Genet, Sandoz Res Inst, Schering Plough Corp, SmithKline Beecham Pharm, Sterling Winthrop Inc, Sumitomo Pharm Co Ltd, Tekeda Chem Ind Ltd, Toyobo Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC C1 NIAAA,INFECT DIS LAB,ROCKVILLE,MD 20852. NR 0 TC 4 Z9 4 U1 0 U2 0 PU COLD SPRING HARBOR LABORATORY PRESS PI PLAINVIEW PA 10 SKYLINE DRIVE, PLAINVIEW, NY 11803-2500 SN 0899-4056 BN 0-87969-467-X J9 VACCINES PY 1995 BP 195 EP 200 PG 6 WC Immunology; Infectious Diseases; Medicine, Research & Experimental SC Immunology; Infectious Diseases; Research & Experimental Medicine GA BD72G UT WOS:A1995BD72G00030 ER PT S AU LEE, L MCHUGH, L SAKUMA, S LOFTUS, D APPELLA, E MARGULIES, DH MAGE, M AF LEE, L MCHUGH, L SAKUMA, S LOFTUS, D APPELLA, E MARGULIES, DH MAGE, M BE Chanock, RM Brown, F Ginsberg, HS Norrby, E TI Peptide-loaded recombinant single-chain HLA molecules as potential immunogens for T cells SO VACCINES 95 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES SE VACCINES (COLD SPRING HARBOR LABORATORY PRESS) LA English DT Proceedings Paper CT 12th Annual Meeting on Molecular Approaches to the Control of Infectious Diseases CY OCT, 1994 CL COLD SPRING HARBOR, NY SP Alafi Capital Co, Amer Cyanamid Co, Amgen Inc, Becton Dickinson & Co, Biogen, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chugai Pharm Co Ltd, Ciba Geigy Corp, Diagnost Prod Corp, Du Pont Merck Pharm Co, Forest Labs Inc, Genentech Inc, Glaxco, Hoffman La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Mitsubishi Kasei Inst Life Sci, Monsanto Co, New England BioLabs Inc, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Pfizer Inc, Res Genet, Sandoz Res Inst, Schering Plough Corp, SmithKline Beecham Pharm, Sterling Winthrop Inc, Sumitomo Pharm Co Ltd, Tekeda Chem Ind Ltd, Toyobo Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC C1 NCI,BIOCHEM LAB,BETHESDA,MD 20892. NR 0 TC 1 Z9 1 U1 0 U2 0 PU COLD SPRING HARBOR LABORATORY PRESS PI PLAINVIEW PA 10 SKYLINE DRIVE, PLAINVIEW, NY 11803-2500 SN 0899-4056 BN 0-87969-467-X J9 VACCINES PY 1995 BP 243 EP 247 PG 5 WC Immunology; Infectious Diseases; Medicine, Research & Experimental SC Immunology; Infectious Diseases; Research & Experimental Medicine GA BD72G UT WOS:A1995BD72G00038 ER PT S AU TSAREV, SA TSAREVA, TS EMERSON, SU PURCELL, RH GOVINDARAJAN, S SHAPIRO, M GERIN, JL AF TSAREV, SA TSAREVA, TS EMERSON, SU PURCELL, RH GOVINDARAJAN, S SHAPIRO, M GERIN, JL BE Chanock, RM Brown, F Ginsberg, HS Norrby, E TI Successful passive and active immunization of cynomolgus monkeys against hepatitis E SO VACCINES 95 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES SE VACCINES (COLD SPRING HARBOR LABORATORY PRESS) LA English DT Proceedings Paper CT 12th Annual Meeting on Molecular Approaches to the Control of Infectious Diseases CY OCT, 1994 CL COLD SPRING HARBOR, NY SP Alafi Capital Co, Amer Cyanamid Co, Amgen Inc, Becton Dickinson & Co, Biogen, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chugai Pharm Co Ltd, Ciba Geigy Corp, Diagnost Prod Corp, Du Pont Merck Pharm Co, Forest Labs Inc, Genentech Inc, Glaxco, Hoffman La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Mitsubishi Kasei Inst Life Sci, Monsanto Co, New England BioLabs Inc, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Pfizer Inc, Res Genet, Sandoz Res Inst, Schering Plough Corp, SmithKline Beecham Pharm, Sterling Winthrop Inc, Sumitomo Pharm Co Ltd, Tekeda Chem Ind Ltd, Toyobo Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC C1 NIAID,INFECT DIS LAB,HEPATITIS VIRUSES SECT,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU COLD SPRING HARBOR LABORATORY PRESS PI PLAINVIEW PA 10 SKYLINE DRIVE, PLAINVIEW, NY 11803-2500 SN 0899-4056 BN 0-87969-467-X J9 VACCINES PY 1995 BP 263 EP 266 PG 4 WC Immunology; Infectious Diseases; Medicine, Research & Experimental SC Immunology; Infectious Diseases; Research & Experimental Medicine GA BD72G UT WOS:A1995BD72G00041 ER PT S AU NARA, P MERGES, M CONLEY, S SHAW, G EZEKOWITZ, A GANDELMAN, H AF NARA, P MERGES, M CONLEY, S SHAW, G EZEKOWITZ, A GANDELMAN, H BE Chanock, RM Brown, F Ginsberg, HS Norrby, E TI Acute-phase response to primary HIV-1 infection in humans SO VACCINES 95 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES SE VACCINES (COLD SPRING HARBOR LABORATORY PRESS) LA English DT Proceedings Paper CT 12th Annual Meeting on Molecular Approaches to the Control of Infectious Diseases CY OCT, 1994 CL COLD SPRING HARBOR, NY SP Alafi Capital Co, Amer Cyanamid Co, Amgen Inc, Becton Dickinson & Co, Biogen, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chugai Pharm Co Ltd, Ciba Geigy Corp, Diagnost Prod Corp, Du Pont Merck Pharm Co, Forest Labs Inc, Genentech Inc, Glaxco, Hoffman La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Mitsubishi Kasei Inst Life Sci, Monsanto Co, New England BioLabs Inc, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Pfizer Inc, Res Genet, Sandoz Res Inst, Schering Plough Corp, SmithKline Beecham Pharm, Sterling Winthrop Inc, Sumitomo Pharm Co Ltd, Tekeda Chem Ind Ltd, Toyobo Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC C1 NCI,FREDERICK CANC RES & DEV CTR,LTCB,VIRUS BIOL SECT,FREDERICK,MD 21702. NR 0 TC 0 Z9 0 U1 0 U2 0 PU COLD SPRING HARBOR LABORATORY PRESS PI PLAINVIEW PA 10 SKYLINE DRIVE, PLAINVIEW, NY 11803-2500 SN 0899-4056 BN 0-87969-467-X J9 VACCINES PY 1995 BP 267 EP 271 PG 5 WC Immunology; Infectious Diseases; Medicine, Research & Experimental SC Immunology; Infectious Diseases; Research & Experimental Medicine GA BD72G UT WOS:A1995BD72G00042 ER PT B AU BADIO, B GATTI, PJ AF BADIO, B GATTI, PJ BE Trouth, CO Millis, RM KiwullSchone, HF Schlafke, ME TI The role of glycine in the pressor response to NMDA injected into the subretrofacial nucleus (SRFN) of the cat SO VENTRAL BRAINSTEM MECHANISMS AND CONTROL OF RESPIRATION AND BLOOD PRESSURE SE LUNG BIOLOGY IN HEALTH AND DISEASE LA English DT Proceedings Paper CT Symposium on Ventral Brainstem Mechanisms and Control of Respiration and Blood Pressure CY NOV 05-06, 1993 CL HOWARD UNIV, WASHINGTON, DC HO HOWARD UNIV C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MARCEL DEKKER PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 BN 0-8247-9514-8 J9 LUNG BIOL HEALTH DIS PY 1995 VL 82 BP 373 EP 380 PG 8 WC Cardiac & Cardiovascular Systems; Neurosciences; Physiology; Respiratory System SC Cardiovascular System & Cardiology; Neurosciences & Neurology; Physiology; Respiratory System GA BD90P UT WOS:A1995BD90P00021 ER PT B AU BOUMPAS, DT AF BOUMPAS, DT BE Boki, KA Drosos, AA Moutsopoulos, HM Tzioufas, AG Vlachoyiannopoulos, PG TI Severe neuropsychiatric lupus: Diagnosis and management SO VII MEDITERRANEAN CONGRESS OF RHEUMATOLOGY LA English DT Proceedings Paper CT VII Mediterranean Congress of Rheumatology - Autoimmune Connective Tissue Disorders CY JUN 16-18, 1994 CL ATHENS, GREECE C1 NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MONDUZZI EDITORE PI 40128 BOLOGNA PA VIA FERRARESE 119/2, 40128 BOLOGNA, ITALY BN 88-323-0616-6 PY 1995 BP 109 EP 113 PG 5 WC Rheumatology SC Rheumatology GA BD84S UT WOS:A1995BD84S00018 ER PT J AU Fomsgaard, A Johnson, PR Nielsen, C Novembre, FJ Hansen, J Goldstein, S Hirsch, VM AF Fomsgaard, A Johnson, PR Nielsen, C Novembre, FJ Hansen, J Goldstein, S Hirsch, VM TI Receptor function of CD4 structures from African green monkey and pig-tail macaque for simian immunodeficiency virus, SIVsm, SIVagm, and human immunodeficiency virus type-1 SO VIRAL IMMUNOLOGY LA English DT Article ID PROTEIN SECONDARY STRUCTURE; INDUCED CELL-FUSION; HIV-1 INFECTION; SYNTHETIC PEPTIDES; BINDING-SITE; I ENVELOPE; GLYCOPROTEIN; RETROVIRUS; REGION; MOLECULES AB Differences in kinetics of infection, cellular tropism, and cytopathology of SIV and HIV appear to depend on both viral and host factors. We investigated the role of critical CD4 structures from African green monkeys (AGM) a natural SIV host, from pig-tailed macaques (PT) an unnatural SIV host, and from humans, as well as the role of species-specific cellular factors involved in the tropism, kinetics of infection, and cytopathic effects of several SIV and HIV. Critical regions of the PT macaque and AGM CD4 genes (V1, V1J1, and V1J1V2J2) were stably expressed as chimeras with the human CD4 gene in human (HeLa and 293) and macaque (CMMT) cell lines. CD4 expressing cell lines were used for infection studies with cell-free SIVsm, SIVmac, SIVsmmPBj, SIVagm, and HIV-1. Results show that both PT CD4 and AGM CD4 supported infection with comparable infection kinetics by all SIV or HIV-1 strains tested. Although structural analysis predicted a major change in secondary structure of AGM CD4/CDR-3, these structural changes did not influence the degree of syncytia formation induced by several SIV and HIV-1. However, the cell line used to express the CD4 gene appeared to be a critical determinant of infection. Thus, SIV strains did not infect human cell lines regardless of the CD4 expressed in these cells. In contrast, HIV-1 did not infect any macaque cell line. This study demonstrates that the differences in CD4 structure among different primate species are clearly not responsible for differences in SIV and HIV infection kinetics, tropism, and cytopathology. However, species-specific factor(s), presumably expressed on the cell surface, markedly influences the ability of SIV or HIV to infect cells expressing CD4. C1 RIGSHOSP,DEPT CLIN MICROBIOL,DK-2100 COPENHAGEN,DENMARK. OHIO STATE UNIV,COLUMBUS,OH 43210. CHILDRENS HOSP RES FDN,COLUMBUS,OH. EMORY UNIV,YERKES REG PRIMATE RES CTR,ATLANTA,GA 30322. TECH UNIV DENMARK,DEPT PHYS CHEM,DK-2800 LYNGBY,DENMARK. NIAID,INFECT DIS LAB,ROCKVILLE,MD. RP Fomsgaard, A (reprint author), STATENS SERUM INST,DEPT VIROL,5 ARTILLERIVEJ,DK-2300 COPENHAGEN S,DENMARK. RI Johnson, Philip/A-6892-2009 NR 42 TC 13 Z9 13 U1 2 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0882-8245 J9 VIRAL IMMUNOL JI Viral Immunol. PY 1995 VL 8 IS 3 BP 121 EP 133 DI 10.1089/vim.1995.8.121 PG 13 WC Immunology; Virology SC Immunology; Virology GA TZ972 UT WOS:A1995TZ97200001 PM 8833265 ER PT J AU Altaner, C Braciale, TJ Cernescu, C Doherty, P Ertl, HCJ Gelman, IH Hilleman, MR Laughlin, CA Liu, MA Reiss, CS Talbot, P Yewdell, JW Zinkernagel, R AF Altaner, C Braciale, TJ Cernescu, C Doherty, P Ertl, HCJ Gelman, IH Hilleman, MR Laughlin, CA Liu, MA Reiss, CS Talbot, P Yewdell, JW Zinkernagel, R TI The experts speak on the future of viral immunology SO VIRAL IMMUNOLOGY LA English DT Article ID AUTOIMMUNITY C1 UNIV VIRGINIA, CHARLOTTESVILLE, VA 22903 USA. INST VIROL, BUCHAREST, ROMANIA. ST JUDE CHILDRENS RES HOSP, DEPT IMMUNOL, MEMPHIS, TN 38105 USA. WISTAR INST ANAT & BIOL, PHILADELPHIA, PA 19104 USA. MT SINAI HOSP, MT SINAI SCH MED, NEW YORK, NY 10029 USA. MERCK RES LABS, MERCK INST THERAPEUT RES, W POINT, PA USA. NIAID, DIV MICROBIOL & INFECT DIS, NIH, BETHESDA, MD 20892 USA. NYU, DEPT BIOL, NEW YORK, NY 10012 USA. UNIV QUEBEC, INST ARMAND FRAPPIER, CTR RECH VIROL, LAVAL, PQ, CANADA. NIAID, LVD, CELLULAR BIOL SECT, NIH, BETHESDA, MD 20892 USA. UNIV ZURICH, INST EXPT IMMUNOL, DEPT PATHOL, CH-8091 ZURICH, SWITZERLAND. TEL AVIV UNIV, TEL HASHOMER & SACKLER FAC MED, DEPT MED B, IL-69978 TEL AVIV, ISRAEL. TEL AVIV UNIV, SHEBA MED CTR, TEL HASHOMER & SACKLER FAC MED, AUTOIMMUNE DIS RES UNIT, IL-69978 TEL AVIV, ISRAEL. RP Altaner, C (reprint author), SLOVAK ACAD SCI, CANC RES INST, BRATISLAVA, SLOVAKIA. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 4 TC 0 Z9 0 U1 0 U2 2 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 0882-8245 J9 VIRAL IMMUNOL JI Viral Immunol. PY 1995 VL 8 IS 4 BP 175 EP 197 PG 23 WC Immunology; Virology SC Immunology; Virology GA VF631 UT WOS:A1995VF63100002 ER PT B AU CANNON, RO AF CANNON, RO BE Gebhart, GF TI Cardiac pain SO VISCERAL PAIN SE PROGRESS IN PAIN RESEARCH AND MANAGEMENT LA English DT Proceedings Paper CT Bristol-Myers-Squibb Symposium on Pain Research - Visceral Pain CY 1994 CL IOWA CITY, IA SP Bristol Myers Squibb C1 NHLBI,CARDIOL BRANCH,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU INT ASSOC STUDY PAIN (IASP) PRESS PI SEATTLE PA 909 NE 43RD ST, SUITE 304, SEATTLE, WA 98105 BN 0-931092-10-8 J9 PROG PAIN RES MANAG PY 1995 VL 5 BP 373 EP 389 PG 17 WC Gastroenterology & Hepatology; Clinical Neurology; Physiology SC Gastroenterology & Hepatology; Neurosciences & Neurology; Physiology GA BD54L UT WOS:A1995BD54L00015 ER PT B AU ALDROUBI, A MCGOWAN, J AF ALDROUBI, A MCGOWAN, J BE Laine, AF Unser, MA TI Oblique and biorthogonal multi-wavelet bases with fast-filtering algorithms SO WAVELET APPLICATIONS IN SIGNAL AND IMAGE PROCESSING III SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT 1995 SPIE Conference on Wavelet Applications in Signal and Image Processing CY JUL 13-14, 1995 CL SAN DIEGO, CA SP Soc Photo Opt Instrumentat Engineers DE MULTI-WAVELET; MULTI-SCALING FUNCTION; OBLIQUE WAVELET BASES; BIORTHOGONAL WAVELET; SEMI-ORTHOGONAL WAVELET; PERFECT RECONSTRUCTION FILTER BANK; PERFECT RESOLVING FILTER BANK; VECTOR FILTER BANK C1 NIH,BIOMED ENGN PROGRAM,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1928-1 J9 P SOC PHOTO-OPT INS PY 1995 VL 2569 BP 15 EP 26 DI 10.1117/12.217567 PN 1&2 PG 12 WC Computer Science, Interdisciplinary Applications; Engineering, Electrical & Electronic; Mathematics, Applied; Optics; Physics, Applied; Radiology, Nuclear Medicine & Medical Imaging SC Computer Science; Engineering; Mathematics; Optics; Physics; Radiology, Nuclear Medicine & Medical Imaging GA BE16C UT WOS:A1995BE16C00002 ER PT B AU UNSER, M AF UNSER, M BE Laine, AF Unser, MA TI A general hilbert space framework for the discretization of continuous signal processing operators SO WAVELET APPLICATIONS IN SIGNAL AND IMAGE PROCESSING III SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT 1995 SPIE Conference on Wavelet Applications in Signal and Image Processing CY JUL 13-14, 1995 CL SAN DIEGO, CA SP Soc Photo Opt Instrumentat Engineers DE CONTINUOUS SIGNAL PROCESSING; SPLINES; BAND-LIMITED FUNCTIONS; WAVELETS; DIFFERENTIATION; OPERATOR DESIGN; FAST ALGORITHMS; RECURSIVE FILTERS; FILTER DESIGN C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1928-1 J9 P SOC PHOTO-OPT INS PY 1995 VL 2569 BP 51 EP 61 DI 10.1117/12.217597 PN 1&2 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Electrical & Electronic; Mathematics, Applied; Optics; Physics, Applied; Radiology, Nuclear Medicine & Medical Imaging SC Computer Science; Engineering; Mathematics; Optics; Physics; Radiology, Nuclear Medicine & Medical Imaging GA BE16C UT WOS:A1995BE16C00005 ER PT B AU VRHEL, MJ LEE, CH UNSER, M AF VRHEL, MJ LEE, CH UNSER, M BE Laine, AF Unser, MA TI Fractal dimension estimation using the fast continuous wavelet transform SO WAVELET APPLICATIONS IN SIGNAL AND IMAGE PROCESSING III SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT 1995 SPIE Conference on Wavelet Applications in Signal and Image Processing CY JUL 13-14, 1995 CL SAN DIEGO, CA SP Soc Photo Opt Instrumentat Engineers C1 NIH,NATL CTR RES RESOURCES,BIOMED ENGN & INSTRUMENTAT PROGRAM,BETHESDA,MD 20892. NR 0 TC 2 Z9 2 U1 0 U2 1 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-1928-1 J9 P SOC PHOTO-OPT INS PY 1995 VL 2569 BP 478 EP 488 DI 10.1117/12.217603 PN 1&2 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Electrical & Electronic; Mathematics, Applied; Optics; Physics, Applied; Radiology, Nuclear Medicine & Medical Imaging SC Computer Science; Engineering; Mathematics; Optics; Physics; Radiology, Nuclear Medicine & Medical Imaging GA BE16C UT WOS:A1995BE16C00042 ER PT J AU BAUMANN, P ROMERO, R AF BAUMANN, P ROMERO, R TI INTRAAMNIOTIC INFECTION, CYTOKINES, AND PRETERM BIRTH SO WIENER KLINISCHE WOCHENSCHRIFT LA German DT Review DE PREGNANCY; PRETERM LABOR; PREMATURITY; INFECTION; CYTOKINES ID TUMOR-NECROSIS-FACTOR; INTERLEUKIN-1 RECEPTOR ANTAGONIST; C-REACTIVE PROTEIN; INTRAAMNIOTIC INFECTION; PREMATURE LABOR; CLINICAL-SIGNIFICANCE; INTACT MEMBRANES; TERM LABOR; BACTERIAL-COLONIZATION; ACUTE PYELONEPHRITIS AB This paper reviews the mechanisms leading to the commencement of labor in the presence of intrauterine infection. It is postulated that induction of preterm contractions represents an escape mechanism of the fetus from a hostile environment. Bacterial toxins provoke an immunological response (macrophages) of the fetomaternal compartment. Liberation of cytokines (IL-I, IL-6, IL-8) stimulates prostanoid synthesis in the decidua and amnion, as well as migration of granulocytes into the cervix. Additional still unknown factors may determine whether this process leads to cervical dilatation, effacement and finally preterm delivery. The role is discussed of other cytokines, i.e., colony-stimulating factors, transforming growth factor beta, and interleukin receptor antagonists as potential, clinically useful tocolytics in this labile equilibrium. C1 WAYNE STATE UNIV,HUTZEL HOSP,NICHD,DETROIT MED CTR,DEPT OBSTET & GYNECOL,PERINATOL RES BRANCH,DETROIT,MI 48202. RP BAUMANN, P (reprint author), UNIV LUBECK,FRAUENHEILKUNDE & GEBURTSHILFE KLIN,RATZEBURGER ALLEE 160,D-23538 LUBECK,GERMANY. NR 107 TC 8 Z9 8 U1 0 U2 3 PU SPRINGER-VERLAG WIEN PI VIENNA PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 VIENNA, AUSTRIA SN 0043-5325 J9 WIEN KLIN WOCHENSCHR JI Wien. Klin. Wochen. PY 1995 VL 107 IS 20 BP 598 EP 607 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA TB432 UT WOS:A1995TB43200002 PM 7502507 ER PT J AU LAROSA, JH AF LAROSA, JH TI WOMEN, WORK, AND COPING - A MULTIDISCIPLINARY APPROACH TO WORKPLACE STRESS - LONG,B, KAHN,S SO WOMEN & HEALTH LA English DT Book Review RP LAROSA, JH (reprint author), NIH,OFF RES WOMENS HLTH,BETHESDA,MD 20892, USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0363-0242 J9 WOMEN HEALTH JI Women Health PY 1995 VL 22 IS 3 BP 79 EP 81 PG 3 WC Public, Environmental & Occupational Health; Women's Studies SC Public, Environmental & Occupational Health; Women's Studies GA QR019 UT WOS:A1995QR01900008 ER PT J AU COMASDIAZ, L JACOBSEN, FM AF COMASDIAZ, L JACOBSEN, FM TI PSYCHOPHARMACOLOGY FOR WOMEN OF COLOR - AN EMPOWERING APPROACH SO WOMEN & THERAPY LA English DT Article ID POPULATIONS; PATIENT; BLACK AB This article reviews the status of psychopharmacology with women of color, It examines both gender and ethnoracial contexts by discussing the dynamics present in the prescription of psychoactive medications to women of color. An empowering approach to psychopharmacology with women of color is presented within the context of a non-physician psychotherapist-physician psychopharmacotherapist concurrent treatment model. In this approach, the therapeutic relationship is designated as a therapeutic triangle, and as a critical agent of change. Emphasis is given to the monitoring and working through of transference and countertransferential reactions in the triangulation of treatment, focusing on ethnocultural acid gender factors. The empowering approach to psychopharmacology views education as an emancipatory psychosocial method aimed at helping women of color manage their situation in a self-affirming manner. C1 TRANSCULTURAL MENTAL HLTH INST,WASHINGTON,DC. GEORGE WASHINGTON UNIV,DEPT PSYCHIAT,WASHINGTON,DC 20052. NIMH,CLIN SCI LAB,BETHESDA,MD 20892. GEORGE WASHINGTON UNIV,FAC MED SCH,WASHINGTON,DC 20052. GEORGETOWN UNIV,FAC MED SCH,WASHINGTON,DC 20057. NR 80 TC 3 Z9 3 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0270-3149 J9 WOMEN THER JI Women Ther. PY 1995 VL 16 IS 1 BP 85 EP 112 DI 10.1300/J015v16n01_06 PG 28 WC Psychology, Multidisciplinary; Women's Studies SC Psychology; Women's Studies GA QW459 UT WOS:A1995QW45900006 ER PT J AU TSAI, SC ADAMIK, R MOSS, J VAUGHAN, M AF TSAI, SC ADAMIK, R MOSS, J VAUGHAN, M TI ADP-RIBOSYLATION FACTORS - ARF STRUCTURE AND FUNCTION SO ZOOLOGICAL STUDIES LA English DT Review DE CHOLERA TOXIN; ADP-RIBOSYLATION FACTORS; GUANINE NUCLEOTIDE-BINDING PROTEINS; BREFELDIN A; PHOSPHOLIPASE D ID GTP-BINDING PROTEINS; GUANINE-NUCLEOTIDE; CHOLERA-TOXIN; BREFELDIN-A; FACTOR-III; ADENYLATE-CYCLASE; GOLGI MEMBRANES; MESSENGER-RNA; FACTOR-I; SACCHAROMYCES-CEREVISIAE AB ADP-ribosylation factors: ARF structure and function. Zoological Studies 34(1): 1-9. ADP-ribosylation factors (ARFs) are similar to 20-kDa guanine nucleotide-binding proteins initially discovered by their ability to activate cholera toxin ADP-ribosyltransferase activity. Subsequently they were shown to be present in the Golgi and to participate in vesicular trafficking in eukaryotic cells. ARFs are highly conserved from Giardia to mammals. Mammalian and Drosophila ARFs fall into three classes based on size, amino acid sequence, and gene structure. The ability of ARFs to activate cholera toxin requires GTP or an analogue and is promoted by phospholipids and detergents. Toxin activation is independent of the amino terminal thirteen amino acids. However, myristoylation at the amino terminus promotes association of ARFs with Golgi and other membranes. Binding to membranes appears to be enhanced by GTP and a soluble complex in a process inhibited by brefeldin A (BFA), a fungal metabolite known to disrupt Golgi. One component of the soluble complex is a phospholipid-dependent guanine nucleotide-exchange protein, which, in a purified state, is independent of BFA and enhances ARF binding of GTP. Activation of ARF by GTP binding promotes its association with Golgi and the subsequent binding of the coatomer proteins, which initiate the vesicular budding process. ARFs have recently been shown to activate phospholipase D which, through the generation of phosphatidate and its metabolites, may be responsible for some of the ARF-initiated membrane trafficking events. RP TSAI, SC (reprint author), NHLBI,CELLULAR METAB LAB,BETHESDA,MD 20892, USA. NR 59 TC 0 Z9 0 U1 0 U2 2 PU ACAD SINICA INST ZOOLOGY PI TAIWAN 115 PA EDITORIAL OFFICE TAIPEI, TAIWAN 115, REP OF CHINA SN 1021-5506 J9 ZOOL STUD JI Zool. Stud. PD JAN PY 1995 VL 34 IS 1 BP 1 EP 9 PG 9 WC Zoology SC Zoology GA QG946 UT WOS:A1995QG94600001 ER PT J AU WITKOP, B BAN, Y AF WITKOP, B BAN, Y TI FROM NATURAL-PRODUCTS TO CURATIVES - REFLECTIONS ON BROSSI,ARNOLD CAREER AND CONTRIBUTIONS SO HETEROCYCLES LA English DT Item About an Individual C1 HOKKAIDO UNIV,SAPPORO,HOKKAIDO 060,JAPAN. RP WITKOP, B (reprint author), NIH,BETHESDA,MD 20892, USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 1 BP 1 EP 8 PG 8 WC Chemistry, Organic SC Chemistry GA QB585 UT WOS:A1994QB58500002 ER PT J AU BANWELL, MG HAMEL, E IRELAND, NK MACKAY, MF AF BANWELL, MG HAMEL, E IRELAND, NK MACKAY, MF TI REGIOCONTROLLED TOTAL SYNTHESES OF THE TROPOLOISOQUINOLINE ALKALOIDS IMERUBRINE AND GRANDIRUBRINE SO HETEROCYCLES LA English DT Article ID DERIVATIVES; ANALOGS AB The previously reported dihydroazafluoranthene (8) has been converted, over a number of steps, into the sigma-homo-o-benzoquinone monoacetal (5). The structure of compound (5) was established by X-ray crystallographic methods and treatment of this material with trifluoroacetic acid resulted in formation of the tropoloisoquinoline alkaloid imerubrine (1). Alternatively, acetal (5) could be hydrolysed to the corresponding diketone (6) which proved to be unstable and isomerised to grandirubrine (2) on heating. Both tropoloisoquinolines (1) and (2) were tested for antitubulin activity, and weak inhibition of polymerisation was observed only with the former compound. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20892. LA TROBE UNIV,DEPT CHEM,BUNDOORA,VIC 3083,AUSTRALIA. RP BANWELL, MG (reprint author), UNIV MELBOURNE,SCH CHEM,PARKVILLE,VIC 3052,AUSTRALIA. RI Banwell, Martin/H-8354-2014 NR 24 TC 14 Z9 14 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 1 BP 205 EP 217 PG 13 WC Chemistry, Organic SC Chemistry GA QB585 UT WOS:A1994QB58500027 ER PT J AU TANGWAI, DF BROSSI, A ARNOLD, LD GROS, P AF TANGWAI, DF BROSSI, A ARNOLD, LD GROS, P TI STRUCTURE-ACTIVITY-RELATIONSHIPS IN THE COLCHICINE MOLECULE WITH RESPECT TO INTERACTION WITH THE MAMMALIAN MULTIDRUG TRANSPORTER, P-GLYCOPROTEIN SO HETEROCYCLES LA English DT Review ID RESISTANT HUMAN-CELLS; EXCHANGING HOMOLOGOUS DOMAINS; MDR GENE FAMILY; FUNCTIONAL-ANALYSIS; TRANSMEMBRANE DOMAIN; BINDING-SITE; MOUSE MDR1; ANALOGS; PROTEINS; MODULATE AB Colchicine forms part of a group of structurally unrelated cytotoxic drugs to which P-glycoprotein overexpression confers resistance to, both in cultured cells in vitro and tumor cells in vivo. Modifications of the methoxy groups on the A and C rings modulated cellular toxicity but had no effect on P-gp interaction. Modifications at the C7 position of the B-ring, in particular the removal of the nitrogen atom of the acetamido group, had a dramatic effect. Examination of calculated molar refractivities (CMR) revealed that only compounds showing CMR values greater than 9.7 were P-gp substrates, suggesting a minimal size requirement for efficient interaction with P-gp. C1 GEORGETOWN UNIV,DEPT CHEM,WASHINGTON,DC 20057. NIDDK,BETHESDA,MD. PFIZER INC,CENT RES,GROTON,CT 06340. RP TANGWAI, DF (reprint author), MCGILL UNIV,DEPT BIOCHEM,3655 DRUMMOND ST,MCINTYRE MED SCI BLDG,MONTREAL,PQ H3G 1Y6,CANADA. NR 50 TC 7 Z9 7 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 1 BP 385 EP 403 PG 19 WC Chemistry, Organic SC Chemistry GA QB585 UT WOS:A1994QB58500042 ER PT J AU BRINGMANN, G GOTZ, R KELLER, PA WALTER, R HENSCHEL, P SCHAFFER, M STABLEIN, M KELLY, TR BOYD, MR AF BRINGMANN, G GOTZ, R KELLER, PA WALTER, R HENSCHEL, P SCHAFFER, M STABLEIN, M KELLY, TR BOYD, MR TI ACETOGENIC ISOQUINOLINE ALKALOIDS .66. FIRST TOTAL SYNTHESIS OF KORUPENSAMINE-A AND KORUPENSAMINE-B SO HETEROCYCLES LA English DT Article ID ANCISTROCLADUS-ABBREVIATUS; DIRECTED SYNTHESIS AB The first total synthesis of korupensamines A (1a) and B (1b), highly polar naphthylisoquinoline alkaloids and, simultaneously, 'monomeric building blocks' of the michellamines, is described. Key step is the Pd-II catalyzed intermolecular biaryl coupling of the two appropriately protected naphthalene and isoquinoline moieties (10) and (11), with the coupling positions activated by bromine and trialkylstannane substituents respectively. C1 BOSTON COLL,DEPT CHEM,CHESTNUT HILL,MA 02167. NCI,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. RP BRINGMANN, G (reprint author), UNIV WURZBURG,INST ORGAN CHEM,HUBLAND,D-97074 WURZBURG,GERMANY. NR 16 TC 38 Z9 38 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 2 BP 503 EP 508 PG 6 WC Chemistry, Organic SC Chemistry GA QC194 UT WOS:A1994QC19400013 ER PT J AU PU, YM ZIFFER, H AF PU, YM ZIFFER, H TI DIASTEREOFACIAL ADDITIONS TO A BETA-SUBSTITUTED GLYCAL, ANHYDRODIHYDROARTEMISININ SO HETEROCYCLES LA English DT Article ID ANTIMALARIAL DRUG; MALARIA AB Triphenylphosphine hydrobromide catalyzed electrophilic addition of ethanol to the carbon-carbon double bond of anhydrodihydroartemisinin occurs predominately from the beta-face of the molecule. RP PU, YM (reprint author), NIH,BLDG 5,ROOM B-35,BETHESDA,MD 20892, USA. NR 10 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 2 BP 649 EP 656 PG 8 WC Chemistry, Organic SC Chemistry GA QC194 UT WOS:A1994QC19400027 ER PT J AU FLIPPENANDERSON, JL GEORGE, C BERTHA, CM RICE, KC AF FLIPPENANDERSON, JL GEORGE, C BERTHA, CM RICE, KC TI X-RAY CRYSTAL-STRUCTURES OF POTENT OPIOID RECEPTOR LIGANDS - ETONITAZENE, CIS-(+)-3-METHYLFENTANYL, ETORPHINE, DIPRENORPHINE, AND BUPRENORPHINE SO HETEROCYCLES LA English DT Article ID PHARMACOLOGICAL CHARACTERIZATION; ABSOLUTE-CONFIGURATION; FUNCTIONAL EXPRESSION; OPIATE RECEPTOR; CLONING; SUBTYPES; AGONIST; TOLERANCE; PEPTIDES; HYDRATE AB As a prelude to molecular modeling and other studies of the newly cloned and expressed mu, delta and kappa opioid receptor subtypes, X-Ray crystal structures were determined for etonitazene, (1) cis-(+)-3-methylfentanyl (4) and etorphine (6), three extremely potent opioid agonists. X-Ray crystal structures were also determined for diprenorphine 7, a potent opioid antagonist, and buprenorphine (8), a clinically useful mixed agonist-antagonist. Agonists (1), (4) and (6) are structurally diverse but have similar profiles while (7) and (8) have substantially different pharmacological profiles but differ structurally by only a methyl vs. a tertbutyl function. The present results should facilitate studies toward understanding the differences which underlie these observations on a molecular basis. C1 NIDDK,MED CHEM LAB,BETHESDA,MD 20982. RP FLIPPENANDERSON, JL (reprint author), USN,RES LAB,STRUCT MATTER LAB,WASHINGTON,DC 20375, USA. NR 49 TC 12 Z9 12 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0385-5414 J9 HETEROCYCLES JI Heterocycles PD DEC 31 PY 1994 VL 39 IS 2 BP 751 EP 766 PG 16 WC Chemistry, Organic SC Chemistry GA QC194 UT WOS:A1994QC19400035 ER PT J AU POMMIER, Y PODDEVIN, B GUPTA, M JENKINS, J AF POMMIER, Y PODDEVIN, B GUPTA, M JENKINS, J TI DNA TOPOISOMERASE-I AND TOPISOMERASE-II CLEAVAGE SITES IN THE TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS (HIV-1) DNA PROMOTER REGION SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID LONG TERMINAL REPEAT; SEQUENCE; CAMPTOTHECIN; TRANSCRIPTION; INHIBITION; AMSACRINE; ABSENCE; DRUGS; BASE AB Topoisomerase sites were mapped in the 5'-long terminal repeat of HIV-1 DNA by agarose and sequencing gel electrophoresis. Topoisomerase II sites were observed in the absence and presence of teniposide and amsacrine in the transcription initiation region and the TATA box, consistent with a possible role of topoisomerase II in transcription. The NF-kB and Sp1 regions were poorly cleaved. Topoisomerase I sites were relatively unfrequent even in the presence of camptothecin. They were absent in the core promoter and were concentrated in the TAR and the upstream region near the junction with the host DNA. (C) 1994 Academic Press, Inc. RP POMMIER, Y (reprint author), NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,MOLEC PHARMACOL LAB,BETHESDA,MD 20892, USA. NR 36 TC 11 Z9 11 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD DEC 30 PY 1994 VL 205 IS 3 BP 1601 EP 1609 DI 10.1006/bbrc.1994.2850 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA PZ997 UT WOS:A1994PZ99700015 PM 7811242 ER PT J AU HIROSE, T SMITH, RJ JETTEN, AM AF HIROSE, T SMITH, RJ JETTEN, AM TI ROR-GAMMA - THE 3RD MEMBER OF ROR-RZR ORPHAN RECEPTOR SUBFAMILY THAT IS HIGHLY EXPRESSED IN SKELETAL-MUSCLE SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID RETINOIC ACID; NUCLEAR RECEPTOR; THYROID-HORMONE; IDENTIFICATION; SUPERFAMILY; BINDING AB In this study, we describe the identification and cloning of a novel member of the nuclear receptor superfamily. This orphan receptor, referred to as ROR gamma, belongs to the ROR/RZR subfamily. The open reading frame of ROR gamma encodes a protein of 560 amino acid residues with a predicted molecular mass of 63 kD. The amino acid sequence of ROR gamma exhibits a 50 and 51% identity with those of ROR alpha/RZR alpha and RZR beta, respectively, whereas the DNA-binding domains were 89% identical. ROR gamma was localized on human chromosome 1. Northern blot analysis using RNA from multiple tissues indicated that ROR gamma is expressed in several tissues but is most highly expressed in skeletal muscle. (C) 1994 Academic Press. Inc. C1 NIEHS,PULM PATHOBIOL LAB,CELL BIOL SECT,RES TRIANGLE PK,NC 27709. RI Hirose, Takahisa /E-6117-2011 NR 18 TC 142 Z9 146 U1 0 U2 4 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD DEC 30 PY 1994 VL 205 IS 3 BP 1976 EP 1983 DI 10.1006/bbrc.1994.2902 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA PZ997 UT WOS:A1994PZ99700067 PM 7811290 ER PT J AU SU, XZ WELLEMS, TE AF SU, XZ WELLEMS, TE TI SEQUENCE, TRANSCRIPT CHARACTERIZATION AND POLYMORPHISMS OF A PLASMODIUM-FALCIPARUM GENE BELONGING TO THE HEAT-SHOCK PROTEIN (HSP)90 FAMILY SO GENE LA English DT Note DE MALARIA; TRANSCRIPTION PROMOTER; MESSENGER-RNA TERMINATION SIGNAL; TRINUCLEOTIDE REPEAT; GENETIC POLYMORPHISM; CHLOROQUINE RESISTANCE; LINKAGE ANALYSIS ID ANTIGEN; EXPRESSION; ORGANIZATION AB A gene (pfhsp86) encoding a member of the heat-shock protein 90 (HSP90) family has been isolated from Plasmodium falciparum (Pf). The pfhsp86 coding region comprises two exons separated by an 0.8-kb intron with consensus splice junction sequences. The transcript itself is 3.4-kb long and includes a 0.65-kb 5'-untranslated region (UTR) and a 0.54-kb 3'-UTR. Upstream of the transcription start point (tsp) are putative promoter modules: an inverted CCAAT box, a G+C-rich sequence and several TATA sequences. Transcription is enhanced in erythrocyte-stage parasites cultivated at elevated temperatures (2-3-fold at 39 degrees C and 3-4-fold at 41 degrees C, relative to 37 degrees C). The pfhsp86 gene maps within a chromosome 7 segment that is linked to chloroquine (Cq) response in a Pf cross. The parents of this cross (Dd2, HB3) differ in the first exon by two trinucleotide repeats, while more divergence is apparent between the introns. These trinucleotide repeat differences are linked to Cq response in the HB3 x Dd2 cross, but they did not predict Cq response in nine Pf lines from different locations. C1 NIAID, MALARIA RES LAB, BETHESDA, MD 20892 USA. OI Su, Xinzhuan/0000-0003-3246-3248 NR 20 TC 32 Z9 34 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD DEC 30 PY 1994 VL 151 IS 1-2 BP 225 EP 230 DI 10.1016/0378-1119(94)90661-0 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA QC648 UT WOS:A1994QC64800037 PM 7828879 ER PT J AU GARDNER, K MOORE, TC DAVISSMYTH, T KRUTZSCH, H LEVENS, D AF GARDNER, K MOORE, TC DAVISSMYTH, T KRUTZSCH, H LEVENS, D TI PURIFICATION AND CHARACTERIZATION OF A MULTICOMPONENT AP-1 JUND COMPLEX FROM T-CELLS - DEPENDENCE ON A SEPARATE CELLULAR FACTOR FOR ENHANCED DNA-BINDING ACTIVITY SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROTEIN-KINASE-C; TRANSCRIPTION FACTOR AP-1; APE LEUKEMIA-VIRUS; FOS-JUN; GENE-EXPRESSION; LYMPHOCYTES-T; HETERODIMER FORMATION; SIGNAL TRANSDUCTION; INTERLEUKIN-2 GENE; MOLECULAR-CLONING AB TAP-1 (T-cell AP-1) is a previously identified DNA-binding activity that is rapidly induced in activated T cells in the absence of protein synthesis, This activity has been purified over 2,000-fold from the T-cell line MLA144. Purified TAP-1 is a multicomponent complex composed of 38-kDa and 43-kDa junD polypeptides in association with a separate factor(s), distinct from fos, that partly dissociate from the complex during affinity purification but is required for full TAP-1 DNA-binding activity. When reconstituted with TAP-1, this partly dissociated component strongly enhances the DNA-binding activity of the TAP-1 complex. W-cross linking analysis identifies the dissociable component of the TAP-1 complex as a separate class of low molecular mass (23-29-kDa) DNA-binding polypeptide(s). 23-29-kDa polypeptides have been partially purified from nuclear extracts derived from MLA144 that enhance TAP-1 DNA-binding activity over 100-fold and increase its contacts with flanking DNA sequence. These results define TAP-1 as a distinct AP-l junD-containing complex in T cells whose DNA-binding activity is regulated by the interaction of distinct and separate cellular factor(s). RP GARDNER, K (reprint author), NCI,PATHOL LAB,BLDG 10,RM 2N212,BETHESDA,MD 20892, USA. RI Levens, David/C-9216-2009 OI Levens, David/0000-0002-7616-922X NR 74 TC 19 Z9 20 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD DEC 30 PY 1994 VL 269 IS 52 BP 32963 EP 32971 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QA638 UT WOS:A1994QA63800039 PM 7806526 ER PT J AU HALLOCK, YF DAI, JR BOKESCH, HR DILLAH, KB MANFREDI, KP CARDELLINA, JH BOYD, MR AF HALLOCK, YF DAI, JR BOKESCH, HR DILLAH, KB MANFREDI, KP CARDELLINA, JH BOYD, MR TI HIV-INHIBITORY NATURAL-PRODUCTS .19. PREPARATIVE SEPARATION OF NAPHTHYLTETRAHYDROISOQUINOLINE ALKALOIDS FROM ANCISTROCLADUS-KORUPENSIS BY CENTRIFUGAL PARTITION CHROMATOGRAPHY SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article AB Crude extracts of Ancistrocladus korupensis contain a complex mixture of naphthyltetrahydroisoquinoline alkaloids, including the human immunodeficiency virus-inhibitory dimeric alkaloids michellamines A and B and the antimalarial monomeric korupensamines A-D. The efficient separation of michellamines A and B from these extracts has been accomplished by centrifugal partition chromatography. The chromatographic conditions used on a multi-channel cartridge unit (Sanki LLN) have been successfully scaled up with a newly developed, stacked-disk type centrifugal partition chromatography unit (Sanki NMF) for separating larger amounts of alkaloid mixtures with similar resolution. A refined, three-step process (solvent-solvent partitioning, centrifugal partition chromatography and HPLC) has been developed and applied to the scaled-up production of michellamine B for preclinical drug development. C1 NCI,DIV CANC TREATMENT,DEV THERAPEUT PROGRAM,DRUG DISCOVERY RES & DEV LAB,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,DYNCORP INC,PROGRAM RESOURCES,FREDERICK,MD 21702. NR 6 TC 15 Z9 16 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 J9 J CHROMATOGR A JI J. Chromatogr. A PD DEC 30 PY 1994 VL 688 IS 1-2 BP 83 EP 88 DI 10.1016/0021-9673(94)00923-6 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA QC795 UT WOS:A1994QC79500008 PM 7894648 ER PT J AU HODES, RJ STRIKAS, RA HILL, JC AF HODES, RJ STRIKAS, RA HILL, JC TI MANAGEMENT OF INFECTIONS CAUSED BY ANTIBIOTIC-RESISTANT STREPTOCOCCUS-PNEUMONIAE SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 CTR DIS CONTROL & PREVENT,ATLANTA,GA 30333. NIAID,BETHESDA,MD 20892. RP HODES, RJ (reprint author), NIA,BETHESDA,MD 20892, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD DEC 29 PY 1994 VL 331 IS 26 BP 1775 EP 1775 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA PZ266 UT WOS:A1994PZ26600017 PM 7984206 ER PT J AU MELNICK, SL SHERER, R LOUIS, TA HILLMAN, D RODRIGUEZ, EM LACKMAN, C CAPPS, L BROWN, LS CARLYN, M KORVICK, JA DEYTON, L AF MELNICK, SL SHERER, R LOUIS, TA HILLMAN, D RODRIGUEZ, EM LACKMAN, C CAPPS, L BROWN, LS CARLYN, M KORVICK, JA DEYTON, L TI SURVIVAL AND DISEASE PROGRESSION ACCORDING TO GENDER OF PATIENTS WITH HIV-INFECTION - THE TERRY-BEIRN-COMMUNITY-PROGRAMS FOR CLINICAL RESEARCH ON AIDS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; NEW-YORK-CITY; UNITED-STATES; NATURAL-HISTORY; WOMEN; EXPERIENCE; EPIDEMIC; MEN; TUBERCULOSIS; ZIDOVUDINE AB Objective.-To compare disease progression and mortality between women and men infected with human immunodeficiency virus (HIV). Design.-Multicenter cohort. Setting.-Seventeen community-based centers participating in the Terry Beirn Community Programs for Clinical Research on AIDS (CPCRA). Patients.-A total of 768 women and 3779 men enrolled in one or more of 11 protocols between September 7, 1990, and September 30, 1993. Main Outcome Measures.-Survival and opportunistic events. Results.-The median CD4(+) cell count at enrollment into the cohort was 0.240 x10(9)/L (240/mu L) for women and 0.137 x10(9)/L for men (P<.001). Compared with men, women were younger (36 vs 38 years), more likely to be African American or Hispanic (78% vs 44%), and more likely to have reported a history of injection drug use (49% vs 27%). Women had been followed up fora median of 14.5 months and men for 15.5 months. The adjusted relative risk (RR) for death among women compared with men was 1.33 (95% confidence interval [CI], 1.06 to 1.67; P=.01) and for disease progression (including death) was 0.97 (95% CI, 0.82 to 1.15; P=.72). Women were at increased risk for bacterial pneumonia (RR, f.38; 95% CI, 1.05 to 1.92) and at reduced risk for the development of Kaposi's sarcoma (RR, 0.16; 95% CI, 0.04 to 0.65) and oral hairy leukoplakia (RR, 0.54; 95% CI, 0.31 to 0.94). The increased risk of death and bacterial pneumonia for women compared with men was primarily evident among those with a history of injection drug use (RR, 1.68 for death, 95% CI, 1.20 to 2.35, P=.003; RR, 1.53 for bacterial pneumonia, 95% CI, 1.03 to 2.29, P=.04). Among patients without a history of disease progression at entry, death was the first event reported for more women than men (27.5% vs 12.2%). Conclusions.-Compared with men, HIV-infected women in the CPCRA were at increased risk of death but not disease progression. Risks of most incident opportunistic diseases were similar for women and men; however, women were at an increased risk of bacterial pneumonia. These findings may reflect differential access to health care and standard treatments or different socioeconomic status and social support for women compared with men. C1 UNIV MINNESOTA,SCH PUBL HLTH,DIV EPIDEMIOL,MINNEAPOLIS,MN 55455. UNIV MINNESOTA,SCH PUBL HLTH,DIV BIOSTAT,MINNEAPOLIS,MN 55455. COOK CTY HOSP,COOK CTY HIV PRIMARY CARE CTR,CHICAGO,IL 60612. BRONX PERINATAL CONSORTIUM,BRONX,NY. COLUMBIA UNIV,HARLEM HOSP CTR,DIV INFECT DIS,NEW YORK,NY. ADDICT RES & TREATMENT CORP,BROOKLYN,NY. COLUMBIA UNIV,HARLEM HOSP MED CTR,COLL PHYS & SURG,NEW YORK,NY. PROSPECT ASSOCIATES,ROCKVILLE,MD. RP MELNICK, SL (reprint author), NIAID,DIV AIDS,EPIDEMIOL BRANCH,6003 EXECUT BLVD,ROOM 2C09,MSC 7620,BETHESDA,MD 20892, USA. NR 46 TC 150 Z9 152 U1 0 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD DEC 28 PY 1994 VL 272 IS 24 BP 1915 EP 1921 DI 10.1001/jama.272.24.1915 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA PX927 UT WOS:A1994PX92700036 PM 7990243 ER PT J AU MURAYAMA, T HEWLETT, EL MALONEY, NJ JUSTICE, JM MOSS, J AF MURAYAMA, T HEWLETT, EL MALONEY, NJ JUSTICE, JM MOSS, J TI EFFECT OF TEMPERATURE AND HOST FACTORS ON THE ACTIVITIES OF PERTUSSIS TOXIN AND BORDETELLA ADENYLATE-CYCLASE SO BIOCHEMISTRY LA English DT Article ID ADP-RIBOSYLATION FACTOR; ADENINE-NUCLEOTIDES; CHOLERA-TOXIN; NAD GLYCOHYDROLASE; ACTIVATION; PROTEINS; BINDING; STIMULATION; GTP; RIBOSYLTRANSFERASE AB Pertussis toxin and adenylate cyclase toxin both contribute to the pathogenesis of whooping cough. Production of these proteins is controlled by the bvg locus, which is inactive at 25 degrees C, but at 37 degrees C produces a Vir(+) phenotype. In view of the temperature dependence of virulence factor synthesis, the effects of temperature and host factors on their action were examined. The NAD glycohydrolase activity of the SZ subunit of pertussis toxin was enhanced by CHAPS, a zwitterionic detergent, with a temperature optimum of similar to 35 degrees C. Similar temperature optima for the ADP-ribosylation by pertussis toxin of transducin and recombinant G(o alpha) were observed. Since the temperature-activity relationship of S1 differed from that of S1 in activated holotoxin, and since S1 in activated holotoxin was more stable at 42 degrees C than was S1, it appears that S1 associated with the B oligomer components may, in fact, be an active species. Bordetella pertussis adenylate cyclase is activated by a host factor, calmodulin. In the absence of calmodulin, the temperature optimum for enzymatic activity was similar to 25 degrees C, whereas in its presence it was similar to 35 degrees C. Thus, the temperature optima for pertussis and adenylate cyclase toxins, virulence factors whose production is increased through the bvg locus at physiological temperatures, are either at or near these temperatures when stimulated by host factors. C1 NHLBI,CELLULAR METAB LAB,BETHESDA,MD 20892. UNIV VIRGINIA,SCH MED,DEPT MED,DIV CLIN PHARMACOL,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,SCH MED,DEPT PHARMACOL,DIV CLIN PHARMACOL,CHARLOTTESVILLE,VA 22908. FU NIAID NIH HHS [R01 AI 18000, R01 AI 31119]; NIDDK NIH HHS [5-T32-DK07320] NR 28 TC 3 Z9 3 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD DEC 27 PY 1994 VL 33 IS 51 BP 15293 EP 15297 DI 10.1021/bi00255a010 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA PZ269 UT WOS:A1994PZ26900010 PM 7803392 ER PT J AU SANNERUD, CA PRADA, J GOLDBERG, DM GOLDBERG, SR AF SANNERUD, CA PRADA, J GOLDBERG, DM GOLDBERG, SR TI THE EFFECTS OF SERTRALINE ON NICOTINE SELF-ADMINISTRATION AND FOOD-MAINTAINED RESPONDING IN SQUIRREL-MONKEYS SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE NICOTINE; SERTRALINE; 5-HT (5-HYDROXYTRYPTAMINE, SEROTONIN); SELF-ADMINISTRATION; (MONKEY) ID SEROTONIN-UPTAKE INHIBITOR; VOLUNTARY ETHANOL-CONSUMPTION; WITHDRAWAL SYMPTOMS; BEHAVIOR; DOPAMINE; BUSPIRONE; RELEASE; RATS; INJECTIONS; SCHEDULE AB Recent reports suggested the involvement of serotonergic mechanisms in nicotine self-administration. The present study assessed the effects of sertraline, a selective serotonergic uptake inhibitor, on the reinforcing effects of i.v. nicotine (30 mu g/kg per injection) in squirrel monkeys responding under a fixed-ratio schedule. Nicotine (10-100 mu g/kg per injection) produced a significant inverted U-shaped distribution on FR rate. Vehicle or sertraline (3, 6, 12, 24 mg/kg, p.o.) produced no changes in the response rates maintained by 30 mu g/kg per injection i.v. nicotine, but sertraline produced non-significant increases response rates maintained by 10 mu g/kg per injection nicotine and vehicle. In a separate group of monkeys, sertraline given in combination with i.m. doses of nicotine produced a significant dose-dependent decrease in responding maintained by food-pellet delivery. Thus, sertraline produced differential effects on response rates that may be related to (1) route of nicotine administration and (2) whether the behavior was maintained by nicotine or food. In addition, the results of the self-administration study suggest that sertraline would not disrupt well-maintained responding for nicotine. C1 NIDA,INTRAMURAL RES PROGRAM,PRECLIN PHARMACOL LAB,BEHAV PHARMACOL & GENET SECT,BALTIMORE,MD 21224. UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,BALTIMORE,MD 21201. NR 31 TC 29 Z9 29 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD DEC 27 PY 1994 VL 271 IS 2-3 BP 461 EP 469 DI 10.1016/0014-2999(94)90807-9 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA PZ562 UT WOS:A1994PZ56200028 PM 7705446 ER PT J AU FUKUYAMA, R WADHWANI, KC GALDZICKI, Z RAPOPORT, SI EHRENSTEIN, G AF FUKUYAMA, R WADHWANI, KC GALDZICKI, Z RAPOPORT, SI EHRENSTEIN, G TI BETA-AMYLOID POLYPEPTIDE INCREASES CALCIUM-UPTAKE IN PC12 CELLS - A POSSIBLE MECHANISM FOR ITS CELLULAR TOXICITY IN ALZHEIMERS-DISEASE SO BRAIN RESEARCH LA English DT Note DE AMYLOID; ALZHEIMERS DISEASE; TOXICITY; BETA-AMYLOID PEPTIDE; PHEOCHROMOCYTOMA CELL ID NERVE GROWTH-FACTOR; PEPTIDE; BINDING; NEURONS AB Calcium-uptake into PC12 cells was measured by incubation with Ca-45 after the cells were exposed for 24 h to beta-amyloid peptide(1-40) at concentrations between 0 and 46 mu M. The rate of influx of Ca-45 into PC12 cells was constant for the first 10 min. For 46 mu M beta-amyloid peptide(1-40), the rate of influx was about 1,300 ions/s/mu m(2) and the number of cells decreased significantly. There was no significant decrease in cell number when cells were exposed to beta-amyloid in calcium-free medium. These results indicate that beta-amyloid increases calcium uptake into PC12 cells, and suggest that the increased uptake is responsible for the toxicity of beta-amyloid in PC12 cells. C1 NINCDS,CLIN NEUROSCI BRANCH,BETHESDA,MD 20892. RP FUKUYAMA, R (reprint author), NIA,NEUROSCI LAB,BLDG 10,RM 6C103,BETHESDA,MD 20892, USA. NR 17 TC 44 Z9 44 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD DEC 26 PY 1994 VL 667 IS 2 BP 269 EP 272 DI 10.1016/0006-8993(94)91505-9 PG 4 WC Neurosciences SC Neurosciences & Neurology GA QA727 UT WOS:A1994QA72700013 PM 7697365 ER PT J AU MINNICK, DT PAVLOV, YI KUNKEL, TA AF MINNICK, DT PAVLOV, YI KUNKEL, TA TI THE FIDELITY OF THE HUMAN LEADING AND LAGGING-STRAND DNA-REPLICATION APPARATUS WITH 8-OXODEOXYGUANOSINE TRIPHOSPHATE SO NUCLEIC ACIDS RESEARCH LA English DT Article ID UV-IRRADIATED DNA; MUTAGENIC SUBSTRATE; CELL-EXTRACTS; 8-HYDROXYGUANINE; POLYMERASES; SPECIFICITY; HYDROLYZES; INITIATION; PROTEIN; INVITRO AB A product of oxidative metabolism, 8-oxodeoxyguanosine triphosphate (8-O-dGTP), readily pairs with adenine during DNA replication, ultimately causing A.T-->C.G transversions. This study utilized 8-O-dGTP as a probe to examine the fidelity of the leading and lagging strand replication apparatus in extracts of HeLa cells. Simian virus (SV) 40 T antigen-dependent DNA replication reactions were performed with two M13mp2 vectors with the SV40 origin located on opposite sides of the lacZ alpha sequence used to score replication errors. The presence of 8-O-dGTP at equimolar concentration with each of the 4 normal dNTPs resulted in a >46-fold increase in error rate for A.T-->C.G transversions over that observed in the absence of 8-O-dGTP. A similar average error rate was observed on the (+) and (-) strands in both vectors, suggesting that the fidelity of replication by leading and lagging strand replication proteins is similar for the dA.8-O-dGMP mispair. Replication fidelity in the presence of 8-O-dGTP was reduced on both strands when an inhibitor of exonucleolytic proofreading (dGMP) was added to the reaction. These data suggest that the majority of dA.8-O-dGMP mispairs are proofread by both leading and lagging strand replication proteins. C1 NIEHS,MOLEC GENET LAB,RES TRIANGLE PK,NC 27709. ST PETERSBURG STATE UNIV,DEPT GENET,ST PETERSBURG 199034,RUSSIA. NR 31 TC 33 Z9 34 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1994 VL 22 IS 25 BP 5658 EP 5664 DI 10.1093/nar/22.25.5658 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QC753 UT WOS:A1994QC75300019 PM 7838719 ER PT J AU YE, JP YOUNG, HA ORTALDO, JR GHOSH, P AF YE, JP YOUNG, HA ORTALDO, JR GHOSH, P TI IDENTIFICATION OF A DNA-BINDING SITE FOR THE NUCLEAR FACTOR YY1 IN THE HUMAN GM-CSF CORE PROMOTER SO NUCLEIC ACIDS RESEARCH LA English DT Article ID COLONY-STIMULATING FACTOR; SERUM RESPONSE FACTOR; TRANSCRIPTION FACTORS; MAMMALIAN-CELLS; NF-E1 YY-1; T-CELLS; C-MYC; ELEMENT; ENHANCER; PROTEIN AB It has been well documented that the repeated CATT(A/T) sequence, localized between -64 and -35 in the human GM-CSF promoter, is required for the promoter activity, and this region likely serves as a core recognition sequence for a cellular transcription factor. However, the transcription factor that interacts with this site was not identified, Here, we report that this element contains a binding site for the nuclear factor YY1, which has not been reported to play a role in the regulation of cytokine gene transcription. Results from transient transfection assays of the Jurkat T cell line revealed that this repeated CATT(A/T) element exhibited enhancer activity when linked to both the human IFN-gamma promoter and the TK promoter. Mutation of the YY1 binding site eliminated about 60% of the enhancer activity of the element. We have found that the YY1 binding site could form two specific DNA-protein complexes, A and B, with Jurkat nuclear proteins in the electrophoretic mobility shift assay and that the binding of these complexes correlates with the enhancer activity. UV cross-linking analysis revealed that the A complex is a multi-protein complex and in addition to YY1, other proteins are required for formation of the protein complex. Cotransfection assays with a YY1 expression vector revealed that overexpression of YY1 resulted in an inhibitory effect on the repeated CATT(A/T) element, indicating that in addition to YY1, cofactors also are required for the activator function of the A complex. C1 NCI,FREDERICK CANC RES & DEV CTR,EXPTL IMMUNOL LAB,BIOL RESPONSE MODIFIER PROGRAM,DCT,FREDERICK,MD 21702. NR 35 TC 34 Z9 34 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 25 PY 1994 VL 22 IS 25 BP 5672 EP 5678 DI 10.1093/nar/22.25.5672 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QC753 UT WOS:A1994QC75300021 PM 7838721 ER EF