FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Takayama, Y Clore, GM AF Takayama, Yuki Clore, G. Marius TI Interplay between Minor and Major Groove-binding Transcription Factors Sox2 and Oct1 in Translocation on DNA Studied by Paramagnetic and Diamagnetic NMR SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MOLECULAR-STRUCTURE DETERMINATION; PROTEIN-PROTEIN ASSOCIATION; RELAXATION ENHANCEMENT; POU DOMAIN; EXCHANGE SPECTROSCOPY; COGNATE SITES; XPLOR-NIH; COMPLEXES; MACROMOLECULES; VISUALIZATION AB The pathways whereby Sox2 scans DNA to locate its specific binding site are investigated by NMR in specific and nonspecific Sox2.DNA complexes and in a specific ternary complex with Oct1 on the Hoxb1 regulatory element. Direct transfer of Sox2 between nonspecific sites on different DNA molecules occurs without dissociation into free solution at a rate of similar to 10(6) M-1 s(-1), whereas one-dimensional sliding proceeds with a diffusion constant of >= 0.1 mu m(2).s(-1). Translocation of Sox2 from one specific DNA site to another occurs via jumping, involving complete dissociation into free solution (k(d)similar to 5-6 s(-1)) followed by reassociation (k(a)similar to 5 x 10(8) M-1 s(-1)). In the presence of Oct1 bound to an adjacent specific site, k(d) is reduced by more than 10-fold. Paramagnetic relaxation measurements, however, demonstrate that sparsely populated (<1%), transient states involving nonspecifically bound Sox2 in rapid exchange with specifically bound Sox2 are sampled in both binary Sox2.DNA- and ternary Oct1.Sox2.Hoxb1-DNA-specific complexes. Moreover, Sox2 modulates the mechanism of translocation of Oct1. Both Sox2 and the Oct1 POUHD domain are transiently released from the specific ternary complex by sliding to an adjacent nonspecific site, followed by direct transfer to another DNA molecule, whereas the Oct1 POUS domain is fixed to its specific site through direct interactions with Sox2. Intermolecular translocation of POUHD results in the formation of a bridged intermediate spanning two DNA molecules, enhancing the probability of complete intermolecular translocation of Oct1. By way of contrast, in the specific Oct1.DNA binary complex, POUS undergoes direct intermolecular translocation, whereas POUHD scans the DNA by sliding. C1 [Takayama, Yuki; Clore, G. Marius] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Clore, GM (reprint author), NIDDK, Chem Phys Lab, NIH, Bldg 5, Bethesda, MD 20892 USA. EM mariusc@intra.niddk.nih.gov RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 FU National Institutes of Health, NIDDK; Office of the Director of the National Institutes of Health; Japan Society for the Promotion of Science FX This work was supported, in whole or in part, by the National Institutes of Health, NIDDK, Intramural Program and by the AIDS Targeted Antiviral Program of the Office of the Director of the National Institutes of Health (to G. M. C.).; Supported by a Japan Society for the Promotion of Science Research Fellowship for Japanese Biomedical and Behavioral Researchers at the National Institutes of Health. NR 37 TC 15 Z9 15 U1 1 U2 8 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 14349 EP 14363 DI 10.1074/jbc.M112.352864 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200004 PM 22396547 ER PT J AU Wickner, RB AF Wickner, Reed B. TI Discovering Protein-based Inheritance through Yeast Genetics SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DOUBLE-STRANDED-RNA; BETA-SHEET STRUCTURE; RIBOSOMAL-SUBUNIT BIOGENESIS; TERMINATION FACTOR ERF3; PRION-INDUCING DOMAIN; L-A VIRUS; SACCHAROMYCES-CEREVISIAE; MESSENGER-RNA; IN-VITRO; ESCHERICHIA-COLI C1 NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. RP Wickner, RB (reprint author), NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. EM wickner@helix.nih.gov NR 128 TC 4 Z9 4 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 14432 EP 14442 DI 10.1074/jbc.X112.355636 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200011 PM 22396539 ER PT J AU Zanou, N Schakman, O Louis, P Ruegg, UT Dietrich, A Birnbaumer, L Gailly, P AF Zanou, Nadege Schakman, Olivier Louis, Pierre Ruegg, Urs T. Dietrich, Alexander Birnbaumer, Lutz Gailly, Philippe TI Trpc1 Ion Channel Modulates Phosphatidylinositol 3-Kinase/Akt Pathway during Myoblast Differentiation and Muscle Regeneration SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GROWTH-FACTOR-I; CELL-CYCLE ARREST; HELIX-LOOP-HELIX; SKELETAL-MUSCLE; MYOGENIN EXPRESSION; MYOCYTE MATURATION; MOUSE EMBRYO; IGF-I; MYOD; ACTIVATION AB We previously showed in vitro that calcium entry through Trpc1 ion channels regulates myoblast migration and differentiation. In the present work, we used primary cell cultures and isolated muscles from Trpc1(-/-) and Trpc1(+/+) murine model to investigate the role of Trpc1 in myoblast differentiation and in muscle regeneration. In these models, we studied regeneration consecutive to cardiotoxin-induced muscle injury and observed a significant hypotrophy and a delayed regeneration in Trpc1(-/-) muscles consisting in smaller fiber size and increased proportion of centrally nucleated fibers. This was accompanied by a decreased expression of myogenic factors such as MyoD, Myf5, and myogenin and of one of their targets, the developmental MHC (MHCd). Consequently, muscle tension was systematically lower in muscles from Trpc1(-/-) mice. Importantly, the PI3K/Akt/mTOR/p70S6K pathway, which plays a crucial role in muscle growth and regeneration, was down-regulated in regenerating Trpc1(-/-) muscles. Indeed, phosphorylation of both Akt and p70S6K proteins was decreased as well as the activation of PI3K, the main upstream regulator of the Akt. This effect was independent of insulin-like growth factor expression. Akt phosphorylation also was reduced in Trpc1(-/-) primary myoblasts and in control myoblasts differentiated in the absence of extracellular Ca2+ or pretreated with EGTA-AM or wortmannin, suggesting that the entry of Ca2+ through Trpc1 channels enhanced the activity of PI3K. Our results emphasize the involvement of Trpc1 channels in skeletal muscle development in vitro and in vivo, and identify a Ca2+-dependent activation of the PI3K/Akt/mTOR/p70S6K pathway during myoblast differentiation and muscle regeneration. C1 [Zanou, Nadege; Schakman, Olivier; Louis, Pierre; Gailly, Philippe] Catholic Univ Louvain, Lab Cell Physiol, Inst Neurosci, B-1200 Brussels, Belgium. [Ruegg, Urs T.] Univ Geneva, Geneva Lausanne Sch Pharmaceut Sci, Pharmacol Lab, CH-1211 Geneva 4, Switzerland. [Dietrich, Alexander] Univ Munich, Walther Straub Inst Pharmakol & Toxikol, D-80336 Munich, Germany. [Birnbaumer, Lutz] Natl Inst Environm Hlth Sci, Neurobiol Lab, Res Triangle Pk, NC 27709 USA. RP Zanou, N (reprint author), Catholic Univ Louvain, Lab Cell Physiol, Inst Neurosci, 55-40 Av Hippocrate, B-1200 Brussels, Belgium. EM nadege.zanou@uclouvain.be; philippe.gailly@uclouvain.be RI Dietrich, Alexander/G-8619-2013; OI Dietrich, Alexander/0000-0002-1168-8707; RUEGG, Urs/0000-0001-6078-8280 FU National Institutes of Health [Z01-101684]; Association francaise contre les myopathies; Association Belge contre les Maladies Neuro-musculaires; General Direction of Scientific Research of the French Community of Belgium [ARC 10/15-029] FX This work was supported, in whole or in part, by the Intramural Research Program of the National Institutes of Health Z01-101684 (to L. B.). This work was also supported by the "Association francaise contre les myopathies", the "Association Belge contre les Maladies Neuro-musculaires", by Grant ARC 10/15-029 from the General Direction of Scientific Research of the French Community of Belgium. NR 50 TC 21 Z9 21 U1 0 U2 15 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 14524 EP 14534 DI 10.1074/jbc.M112.341784 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200019 PM 22399301 ER PT J AU Brzeska, H Guag, J Preston, GM Titus, MA Korn, ED AF Brzeska, Hanna Guag, Jake Preston, G. Michael Titus, Margaret A. Korn, Edward D. TI Molecular Basis of Dynamic Relocalization of Dictyostelium Myosin IB SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PLECKSTRIN HOMOLOGY DOMAIN; PLASMA-MEMBRANE; IN-VIVO; FUSION PROTEINS; ARP2/3 COMPLEX; CELL-MIGRATION; LIVING CELLS; SH3 DOMAIN; HELA-CELLS; TEDS RULE AB Class I myosins have a single heavy chain comprising an N-terminal motor domain with actin-activated ATPase activity and a C-terminal globular tail with a basic region that binds to acidic phospholipids. These myosins contribute to the formation of actin-rich protrusions such as pseudopodia, but regulation of the dynamic localization to these structures is not understood. Previously, we found that Acanthamoeba myosin IC binds to acidic phospholipids in vitro through a short sequence of basic and hydrophobic amino acids, BH site, based on the charge density of the phospholipids. The tail of Dictyostelium myosin IB (DMIB) also contains a BH site. We now report that the BH site is essential for DMIB binding to the plasma membrane and describe the molecular basis of the dynamic relocalization of DMIB in live cells. Endogenous DMIB is localized uniformly on the plasma membrane of resting cells, at active protrusions and cell-cell contacts of randomly moving cells, and at the front of motile polarized cells. The BH site is required for association of DMIB with the plasma membrane at all stages where it colocalizes with phosphoinositide bisphosphate/phosphoinositide trisphosphate (PIP2/PIP3). The charge-based specificity of the BH site allows for in vivo specificity of DMIB for PIP2/PIP3 similar to the PH domain-based specificity of other class I myosins. However, DMIB-head is required for relocalization of DMIB to the front of migrating cells. Motor activity is not essential, but the actin binding site in the head is important. Thus, dynamic relocalization of DMIB is determined principally by the local PIP2/PIP3 concentration in the plasma membrane and cytoplasmic F-actin. C1 [Brzeska, Hanna; Guag, Jake; Preston, G. Michael; Korn, Edward D.] NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. [Titus, Margaret A.] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA. RP Brzeska, H (reprint author), NHLBI, Cell Biol Lab, NIH, 9000 Rockville Pike,Bldg 50,Rm 2515, Bethesda, MD 20892 USA. EM brzeskah@mail.nih.gov FU National Institutes of Health [GM046486]; NHLBI Intramural Research, National Institutes of Health FX This work was supported, in whole or in part, by National Institutes of Health Grant GM046486 (to M. A. T.). This work was also supported by the NHLBI Intramural Research Program, National Institutes of Health. NR 85 TC 9 Z9 9 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 14923 EP 14936 DI 10.1074/jbc.M111.318667 PG 14 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200055 PM 22367211 ER PT J AU Yu, X Luo, Y Dinkel, P Zheng, J Wei, GH Margittai, M Nussinov, R Ma, BY AF Yu, Xiang Luo, Yin Dinkel, Paul Zheng, Jie Wei, Guanghong Margittai, Martin Nussinov, Ruth Ma, Buyong TI Cross-seeding and Conformational Selection between Three- and Four-repeat Human Tau Proteins SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PAIRED HELICAL FILAMENTS; MOLECULAR-DYNAMICS SIMULATIONS; BETA-STRUCTURE; ALZHEIMERS-DISEASE; NEURODEGENERATIVE-DISEASES; IN-VITRO; AGGREGATION; SHEET; ISOFORMS; PEPTIDE AB In Alzheimer's disease and frontotemporal dementias, the microtubule-associated protein Tau forms intracellular paired helical filaments. The filaments can form not only by the full-length human Tau protein, but also by the three repeated (K19) or four repeated (K18) Tau segments. However, of interest, experimentally, K19 can seed K18, but not vice versa. To obtain insight into the cross-seeding between K18 and K19 aggregates, here, K18 and K19 octamers with repeat 3 (R3) in U-shaped, L-shaped, and long straight line-shaped (SL-shape) conformations are assembled into different structures. The simulation results show that K18-8/K19-8 (K18 and K19 assemblies number 8) with R3 in an L shape and K18-9/K19-9 with R3 in an SL shape are highly populated and present the highest structural similarity among all simulated K18 and K19 octamers, suggesting that similar folding of K18/K19 may serve as structural core for the K18-K19 co-assembled heterogeneous filament. We demonstrate that formation of stable R2 and R3 conformations is the critical step for K18 aggregation, and R3 is critical for K19 fibrillization. The different core units in K18 and K19 may create a cross-seeding barrier for the K18 seed to trigger K19 fibril growth because R2 is not available for K19. Our study provides insights into cross-seeding involving heterogeneous structures. The polymorphic nature of protein aggregation could be magnified in the cross-seeding process. If the seeding conformations lead to too much divergence in the energy landscape, it could impede fibril formation. Such an effect could also contribute to the asymmetric barrier between K18 and K19. C1 [Yu, Xiang; Luo, Yin; Zheng, Jie] Univ Akron, Dept Chem & Biomol Engn, Akron, OH 44325 USA. [Luo, Yin; Wei, Guanghong] Fudan Univ, State Key Lab Surface Phys, Key Lab Computat Phys Sci, Minist Educ, Shanghai 200433, Peoples R China. [Luo, Yin; Wei, Guanghong] Fudan Univ, Dept Phys, Shanghai 200433, Peoples R China. [Dinkel, Paul; Margittai, Martin] Univ Denver, Dept Chem & Biochem, Denver, CO 80208 USA. [Nussinov, Ruth; Ma, Buyong] NCI, Basic Res Program, SAIC Frederick Inc, Ctr Canc Res Nanobiol Program, Frederick, MD 21702 USA. [Nussinov, Ruth] Tel Aviv Univ, Dept Human Genet & Mol Med, Sackler Inst Mol Med, Sackler Sch Med, IL-69978 Tel Aviv, Israel. RP Zheng, J (reprint author), Univ Akron, Dept Chem & Biomol Engn, Akron, OH 44325 USA. EM zhengj@uakron.edu; ghwei@fudan.edu.cn; ruthnu@helix.nih.gov RI Ma, Buyong/F-9491-2011; Zheng, Jie/B-5057-2013; Margittai, Martin/D-5039-2014; Yu, Xiang/A-9765-2012 OI Ma, Buyong/0000-0002-7383-719X; Zheng, Jie/0000-0003-1547-3612; Margittai, Martin/0000-0003-1903-5927; Yu, Xiang/0000-0002-0486-1110 FU National Institutes of Health [R01NS076619]; National Cancer Institute [HHSN261200800001E]; National Cancer Institute Center for Cancer Research, National Science Foundation [CBET-0952624]; National Natural Science Foundation of China [11074047]; Research Fund for the Doctoral Program of Higher Education of China [RFDP-20100071110006] FX This work was supported, in whole or in part, by National Institutes of Health Grant R01NS076619 (to M. M.). This work was also supported by National Cancer Institute Contract HHSN261200800001E, the intramural research program of the National Cancer Institute Center for Cancer Research, National Science Foundation CAREER Award CBET-0952624 (to J. Z.), a 3M nontenured faculty award (to J. Z.), National Natural Science Foundation of China Grant 11074047 (to G. W.), and Research Fund for the Doctoral Program of Higher Education of China Grant RFDP-20100071110006 (to G. W.). NR 42 TC 28 Z9 28 U1 1 U2 22 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 14950 EP 14959 DI 10.1074/jbc.M112.340794 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200057 PM 22393063 ER PT J AU Dogo-Isonagie, C Lam, S Gustchina, E Acharya, P Yang, YP Shahzad-ul-Hussan, S Clore, GM Kwong, PD Bewley, CA AF Dogo-Isonagie, Cajetan Lam, Son Gustchina, Elena Acharya, Priyamvada Yang, Yongping Shahzad-ul-Hussan, Syed Clore, G. Marius Kwong, Peter D. Bewley, Carole A. TI Peptides from Second Extracellular Loop of C-C Chemokine Receptor Type 5 (CCR5) Inhibit Diverse Strains of HIV-1 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; PROTEIN-COUPLED RECEPTOR; ENVELOPE GLYCOPROTEIN; CORECEPTOR ACTIVITY; GP120 BINDING; N-TERMINUS; MONOCLONAL-ANTIBODIES; CRYSTAL-STRUCTURE; ENTRY INHIBITORS; MEMBRANE-FUSION AB To initiate HIV entry, the HIV envelope protein gp120 must engage its primary receptor CD4 and a coreceptor CCR5 or CXCR4. In the absence of a high resolution structure of a gp120-coreceptor complex, biochemical studies of CCR5 have revealed the importance of its N terminus and second extracellular loop (ECL2) in binding gp120 and mediating viral entry. Using a panel of synthetic CCR5 ECL2-derived peptides, we show that the C-terminal portion of ECL2 (2C, comprising amino acids Cys-178 to Lys-191) inhibit HIV-1 entry of both CCR5- and CXCR4-using isolates at low micromolar concentrations. In functional viral assays, these peptides inhibited HIV-1 entry in a CD4-independent manner. Neutralization assays designed to measure the effects of CCR5 ECL2 peptides when combined with either with the small molecule CD4 mimetic NBD-556, soluble CD4, or the CCR5 N terminus showed additive inhibition for each, indicating that ECL2 binds gp120 at a site distinct from that of N terminus and acts independently of CD4. Using saturation transfer difference NMR, we determined the region of CCR5 ECL2 used for binding gp120, showed that it can bind to gp120 from both R5 and X4 isolates, and demonstrated that the peptide interacts with a CD4-gp120 complex in a similar manner as to gp120 alone. As the CCR5 N terminus-gp120 interactions are dependent on CD4 activation, our data suggest that gp120 has separate binding sites for the CCR5 N terminus and ECL2, the ECL2 binding site is present prior to CD4 engagement, and it is conserved across CCR5-and CXCR4-using strains. These peptides may serve as a starting point for the design of inhibitors with broad spectrum anti-HIV activity. C1 [Dogo-Isonagie, Cajetan; Lam, Son; Shahzad-ul-Hussan, Syed; Bewley, Carole A.] NIDDK, Bioorgan Chem Lab, Bethesda, MD 20892 USA. [Gustchina, Elena; Clore, G. Marius] NIDDK, Chem Phys Lab, Bethesda, MD 20892 USA. [Acharya, Priyamvada; Yang, Yongping; Shahzad-ul-Hussan, Syed; Kwong, Peter D.] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. RP Bewley, CA (reprint author), NIDDK, Bioorgan Chem Lab, 8 Ctr Dr, Bethesda, MD 20892 USA. EM caroleb@mail.nih.gov RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 FU National Institutes of Health (NIDDK and NIAID); Office of the Director, National Institutes of Health; Intramural AIDS Research Fellowship FX This work was supported by the Intramural Research Program, National Institutes of Health (NIDDK and NIAID), and the Intramural AIDS Targeted Antiviral Program, Office of the Director, National Institutes of Health (to C. A. B. and G. M. C.).; Recipient of an Intramural AIDS Research Fellowship award. NR 56 TC 12 Z9 12 U1 2 U2 16 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 27 PY 2012 VL 287 IS 18 BP 15076 EP 15086 DI 10.1074/jbc.M111.332361 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941XU UT WOS:000304003200069 PM 22403408 ER PT J AU Chen, CY Chi, YH Mutalif, RA Starost, MF Myers, TG Anderson, SA Stewart, CL Jeang, KT AF Chen, Chia-Yen Chi, Ya-Hui Mutalif, Rafidah Abdul Starost, Matthew F. Myers, Timothy G. Anderson, Stasia A. Stewart, Colin L. Jeang, Kuan-Teh TI Accumulation of the Inner Nuclear Envelope Protein Sun1 Is Pathogenic in Progeric and Dystrophic Laminopathies SO CELL LA English DT Article ID HUTCHINSON-GILFORD-PROGERIA; ENCODING LAMIN A/C; GIRDLE MUSCULAR-DYSTROPHY; CHARCOT-MARIE-TOOTH; A-TYPE LAMINS; ALZHEIMERS-DISEASE; MISSENSE MUTATION; MICE; GENE; MIGRATION AB Human LMNA gene mutations result in laminopathies that include Emery-Dreifuss muscular dystrophy (AD-EDMD) and Hutchinson-Gilford progeria, the premature aging syndrome (HGPS). The Lmna null (Lmna(-/-)) and progeroid Lmna Delta 9 mutant mice are models for AD-EDMD and HGPS, respectively. Both animals develop severe tissue pathologies with abbreviated life spans. Like HGPS cells, Lmna(-/-) and Lmna Delta 9 fibroblasts have typically misshapen nuclei. Unexpectedly, Lmna(-/-) or Lmna Delta 9 mice that are also deficient for the inner nuclear membrane protein Sun1 show markedly reduced tissue pathologies and enhanced longevity. Concordantly, reduction of SUN1 overaccumulation in LMNA mutant fibroblasts and in cells derived from HGPS patients corrected nuclear defects and cellular senescence. Collectively, these findings implicate Sun1 protein accumulation as a common pathogenic event in Lmna(-/-), Lmna Delta 9, and HGPS disorders. C1 [Chi, Ya-Hui] Natl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan 35053, Taiwan. [Chen, Chia-Yen; Jeang, Kuan-Teh] NIAID, Natl Inst Hlth, Bethesda, MD 20892 USA. [Starost, Matthew F.] Natl Inst Hlth, Div Vet Resources, Bethesda, MD 20892 USA. [Myers, Timothy G.] NIAID, Genom Technol Sect, Natl Inst Hlth, Bethesda, MD 20892 USA. [Anderson, Stasia A.] Natl Heart Lung & Blood Inst Anim MRI Core, Natl Inst Hlth, Bethesda, MD 20892 USA. [Mutalif, Rafidah Abdul; Stewart, Colin L.] Inst Med Biol, Singapore 138648, Singapore. [Stewart, Colin L.] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore. RP Chi, YH (reprint author), Natl Hlth Res Inst, Inst Cellular & Syst Med, Zhunan 35053, Taiwan. EM ychi@nhri.org.tw; kjeang@nih.gov RI Jeang, Kuan-Teh/A-2424-2008; Chi, Ya-Hui/B-1080-2010 FU NIAID; IATAP, NIAID; NHRI, Taiwan [NHRI 99A1-CSPP11-014]; NSC, Taiwan [NSC 98-2320-B-400-009-MY3]; Singapore Biomedical Research Council; Agency for Science, Technology and Research (AstarSTAR) FX Work was supported by NIAID intramural funds, the IATAP, NIAID contract to SoBran, Inc., the NHRI, Taiwan (NHRI 99A1-CSPP11-014), and NSC, Taiwan (NSC 98-2320-B-400-009-MY3), and the Singapore Biomedical Research Council and Agency for Science, Technology and Research (AstarSTAR). We thank S.-Y. Chuang, E. Miyagi, J.M. Ward, L. I. Cheng, Z.J. Chen, J. Kabat, S. Becker, S. Bradford, Q. Su, and D. Donahue for assistance; and W. Leonard, J. Hanover, A. Dayton, Y.B. Shi, and D. Camerini-Otero for reading the manuscript. NR 57 TC 59 Z9 61 U1 1 U2 11 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 J9 CELL JI Cell PD APR 27 PY 2012 VL 149 IS 3 BP 565 EP 577 DI 10.1016/j.cell.2012.01.059 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 934KN UT WOS:000303443100009 PM 22541428 ER PT J AU Talkowski, ME Rosenfeld, JA Blumenthal, I Pillalamarri, V Chiang, C Heilbut, A Ernst, C Hanscom, C Rossin, E Lindgren, AM Pereira, S Ruderfer, D Kirby, A Ripke, S Harris, DJ Lee, JH Ha, K Kim, HG Solomon, BD Gropman, AL Lucente, D Sims, K Ohsumi, TK Borowsky, ML Loranger, S Quade, B Lage, K Miles, J Wu, BL Shen, YP Neale, B Shaffer, LG Daly, MJ Morton, CC Gusella, JF AF Talkowski, Michael E. Rosenfeld, Jill A. Blumenthal, Ian Pillalamarri, Vamsee Chiang, Colby Heilbut, Adrian Ernst, Carl Hanscom, Carrie Rossin, Elizabeth Lindgren, Amelia M. Pereira, Shahrin Ruderfer, Douglas Kirby, Andrew Ripke, Stephan Harris, David J. Lee, Ji-Hyun Ha, Kyungsoo Kim, Hyung-Goo Solomon, Benjamin D. Gropman, Andrea L. Lucente, Diane Sims, Katherine Ohsumi, Toshiro K. Borowsky, Mark L. Loranger, Stephanie Quade, Bradley Lage, Kasper Miles, Judith Wu, Bai-Lin Shen, Yiping Neale, Benjamin Shaffer, Lisa G. Daly, Mark J. Morton, Cynthia C. Gusella, James F. TI Sequencing Chromosomal Abnormalities Reveals Neurodevelopmental Loci that Confer Risk across Diagnostic Boundaries SO CELL LA English DT Article ID AUTISM SPECTRUM DISORDERS; PITT-HOPKINS-SYNDROME; CHROMATIN REMODELING FACTOR; GENOME-WIDE ASSOCIATION; MICRODELETION SYNDROME; DEVELOPMENTAL DELAY; SUSCEPTIBILITY LOCI; BIPOLAR DISORDER; CHD8 INTERACTS; SCHIZOPHRENIA AB Balanced chromosomal abnormalities (BCAs) represent a relatively untapped reservoir of single-gene disruptions in neurodevelopmental disorders (NDDs). We sequenced BCAs in patients with autism or related NDDs, revealing disruption of 33 loci in four general categories: (1) genes previously associated with abnormal neurodevelopment (e. g., AUTS2, FOXP1, and CDKL5), (2) single-gene contributors to microdeletion syndromes (MBD5, SATB2, EHMT1, and SNURF-SNRPN), (3) novel risk loci (e. g., CHD8, KIRREL3, and ZNF507), and (4) genes associated with later-onset psychiatric disorders (e. g., TCF4, ZNF804A, PDE10A, GRIN2B, and ANK3). We also discovered among neurodevelopmental cases a profoundly increased burden of copy-number variants from these 33 loci and a significant enrichment of polygenic risk alleles from genome-wide association studies of autism and schizophrenia. Our findings suggest a polygenic risk model of autism and reveal that some neurodevelopmental genes are sensitive to perturbation by multiple mutational mechanisms, leading to variable phenotypic outcomes that manifest at different life stages. C1 [Talkowski, Michael E.; Blumenthal, Ian; Pillalamarri, Vamsee; Chiang, Colby; Heilbut, Adrian; Ernst, Carl; Hanscom, Carrie; Rossin, Elizabeth; Ruderfer, Douglas; Kirby, Andrew; Ripke, Stephan; Lee, Ji-Hyun; Lucente, Diane; Sims, Katherine; Shen, Yiping; Neale, Benjamin; Daly, Mark J.; Gusella, James F.] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. [Rossin, Elizabeth; Kirby, Andrew; Ripke, Stephan; Lage, Kasper; Neale, Benjamin; Daly, Mark J.] Massachusetts Gen Hosp, Analyt & Translat Genet Unit, Boston, MA 02114 USA. [Ohsumi, Toshiro K.; Borowsky, Mark L.] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA. [Quade, Bradley; Wu, Bai-Lin; Shen, Yiping; Morton, Cynthia C.] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA. [Talkowski, Michael E.] Harvard Univ, Sch Med, Dept Neurol, Boston, MA 02115 USA. [Gusella, James F.] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA. [Talkowski, Michael E.; Rossin, Elizabeth; Ruderfer, Douglas; Kirby, Andrew; Ripke, Stephan; Lage, Kasper; Neale, Benjamin; Daly, Mark J.; Morton, Cynthia C.; Gusella, James F.] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02143 USA. [Rosenfeld, Jill A.; Shaffer, Lisa G.] PerkinElmer Inc, Signature Genom Labs, Spokane, WA 99207 USA. [Lindgren, Amelia M.; Pereira, Shahrin; Morton, Cynthia C.] Brigham & Womens Hosp, Dept Obstet, Boston, MA 02115 USA. [Lindgren, Amelia M.; Pereira, Shahrin; Morton, Cynthia C.] Brigham & Womens Hosp, Dept Gynecol, Boston, MA 02115 USA. [Lindgren, Amelia M.; Pereira, Shahrin; Morton, Cynthia C.] Brigham & Womens Hosp, Dept Reprod Biol, Boston, MA 02115 USA. [Harris, David J.] Childrens Hosp Boston, Div Clin Genet, Boston, MA 02115 USA. [Wu, Bai-Lin; Shen, Yiping] Childrens Hosp Boston, Dept Lab Med, Boston, MA 02115 USA. [Ha, Kyungsoo] Georgia Hlth Sci Univ, Canc Res Ctr, Augusta, GA 30912 USA. [Kim, Hyung-Goo] Georgia Hlth Sci Univ, Inst Mol Med & Genet, Dept Obstet & Gynecol, Augusta, GA 30912 USA. [Solomon, Benjamin D.] NHGRI, Med Genet Branch, Bethesda, MD 20892 USA. [Gropman, Andrea L.] Childrens Natl Med Ctr, Dept Neurol, Washington, DC 20010 USA. [Gropman, Andrea L.] George Washington Univ Hlth Sci, Dept Neurol, Washington, DC 20052 USA. [Loranger, Stephanie; Daly, Mark J.; Gusella, James F.] Autism Consortium Boston, Boston, MA 02115 USA. [Lage, Kasper] Massachusetts Gen Hosp, MassGen Hosp Children, Pediat Surg Res Labs, Boston, MA 02114 USA. [Lage, Kasper] Tech Univ Denmark, Ctr Biol Sequence Anal, Dept Syst Biol, DK-2800 Lyngby, Denmark. [Lage, Kasper] Univ Copenhagen, Ctr Prot Res, DK-1165 Copenhagen, Denmark. [Miles, Judith] Univ Missouri Hosp & Clin, Thompson Ctr Autism & Neurodev Disorders, Dept Pediat, Columbia, MO 65201 USA. [Miles, Judith] Univ Missouri Hosp & Clin, Thompson Ctr Autism & Neurodev Disorders, Dept Med Genet, Columbia, MO 65201 USA. [Miles, Judith] Univ Missouri Hosp & Clin, Thompson Ctr Autism & Neurodev Disorders, Dept Pathol, Columbia, MO 65201 USA. [Wu, Bai-Lin] Fudan Univ, Childrens Hosp, Shanghai 200032, Peoples R China. [Wu, Bai-Lin] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China. [Shen, Yiping] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Shanghai 200025, Peoples R China. RP Gusella, JF (reprint author), Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. EM gusella@helix.mgh.harvard.edu RI Heilbut, Adrian/J-9427-2012; Ruderfer, Douglas/M-5795-2016; OI Heilbut, Adrian/0000-0002-8169-7967; Ruderfer, Douglas/0000-0002-2365-386X; Chiang, Colby/0000-0002-4113-6065 FU National Institutes of Health [GM061354, HD065286]; Simons Foundation Autism Research Initiative; Autism Speaks; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health and Human Services; National Institute of Mental Health National Research Service [MH087123]; Massachusetts General Hospital Executive Committee FX We are grateful to all participating subjects and families and to the many healthcare professionals who have contributed to this study, including Mary-Alice Abbott, Darius J. Adams, Kwame Anyane-Yeboa, Stephen G. Bamforth, Tina Bartell, David P. Bick, Joann N. Bodurtha, Carol Clericuzio, Stephanie Cohen, Kristin Dalton, Maria Descartes, Joanne Milisa Drautz, Dawn L. Earl, Luis F. Escobar, Shannon Gerner, Edwin Guzman, Kenneth Handelman, Tim Heshka, Robert J. Hopkin, Micheil Innes, Debby Lambert, Emmanuelle Lemyre, Cynthia Lim, Livija Medne, Graciela Moya, Katie Rutledge, Wendy Smith, Mark Stephan, Darci Sternen, Katie Stoll, Paulien van Galen, Nancy J. Van Vranken, Erica Wahl, Susan E. Wiley, Amy L. White, Anne Woods, and Elaine H. Zackai. The invaluable control data for this study were provided by Pamela Sklar, Shaun Purcell, the International Schizophrenia Consortium, the Wellcome Trust Case Control Consortium, Evan Eichler, Bradley Coe, and Greg Cooper. We thank Tammy Gillis, Mary Anne Anderson, Jayla Ruliera, and Thon de Boer for technical assistance. We also thank Dennis Gurgul, Nilay Roy, and Brent Richter of Partners Research Computing at Massachusetts General Hospital, and contributing staff from Signature Genomic Laboratories and Children's Hospital Boston. This work was funded by grants GM061354 and HD065286 from the National Institutes of Health, the Simons Foundation Autism Research Initiative, and Autism Speaks. This research was also supported by the Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health and Human Services. M. T. was supported by a National Institute of Mental Health National Research Service Award (MH087123) and an Massachusetts General Hospital Executive Committee on Research Fund for Medical Discovery Award. L. G. S. and J. A. R. are employees of Signature Genomic Laboratories, PerkinElmer. NR 45 TC 220 Z9 227 U1 3 U2 41 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 J9 CELL JI Cell PD APR 27 PY 2012 VL 149 IS 3 DI 10.1016/j.cell.2012.03.028 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 934KN UT WOS:000303443100006 PM 22521361 ER PT J AU Langer, HF Choi, EY Zhou, H Schleicher, R Chung, KJ Tang, ZS Gobel, K Bdeir, K Chatzigeorgiou, A Wong, C Bhatia, S Kruhlak, MJ Rose, JW Burns, JB Hill, KE Qu, HC Zhang, YQ Lehrmann, E Becker, KG Wang, YM Simon, DI Nieswandt, B Lambris, JD Li, XR Meuth, SG Kubes, P Chavakis, T AF Langer, Harald F. Choi, Eun Young Zhou, Hong Schleicher, Rebecca Chung, Kyoung-Jin Tang, Zhongshu Goebel, Kerstin Bdeir, Khalil Chatzigeorgiou, Antonios Wong, Connie Bhatia, Sumeena Kruhlak, Michael J. Rose, John W. Burns, James B. Hill, Kenneth E. Qu, Hongchang Zhang, Yongqing Lehrmann, Elin Becker, Kevin G. Wang, Yunmei Simon, Daniel I. Nieswandt, Bernhard Lambris, John D. Li, Xuri Meuth, Sven G. Kubes, Paul Chavakis, Triantafyllos TI Platelets Contribute to the Pathogenesis of Experimental Autoimmune Encephalomyelitis SO CIRCULATION RESEARCH LA English DT Article DE platelets; experimental autoimmune encephalomyelitis; vascular inflammation; autoimmune disease ID CENTRAL-NERVOUS-SYSTEM; GLYCOPROTEIN IB-ALPHA; INFLAMMATORY CELL RECRUITMENT; LEUKOCYTE INTEGRIN MAC-1; MULTIPLE-SCLEROSIS; T-CELLS; PROGENITOR CELLS; VESSEL WALL; P-SELECTIN; IN-VITRO AB Rationale: Multiple sclerosis (MS) and its mouse model, experimental autoimmune encephalomyelitis (EAE), are inflammatory disorders of the central nervous system (CNS). The function of platelets in inflammatory and autoimmune pathologies is thus far poorly defined. Objective: We addressed the role of platelets in mediating CNS inflammation in EAE. Methods and Results: We found that platelets were present in human MS lesions as well as in the CNS of mice subjected to EAE but not in the CNS from control nondiseased mice. Platelet depletion at the effector-inflammatory phase of EAE in mice resulted in significantly ameliorated disease development and progression. EAE suppression on platelet depletion was associated with reduced recruitment of leukocytes to the inflamed CNS, as assessed by intravital microscopy, and with a blunted inflammatory response. The platelet-specific receptor glycoprotein Ib alpha (GPIb alpha) promotes both platelet adhesion and inflammatory actions of platelets and targeting of GPIb alpha attenuated EAE in mice. Moreover, targeting another platelet adhesion receptor, glycoprotein IIb/IIIa (GPIIb/IIIa), also reduced EAE severity in mice. Conclusions: Platelets contribute to the pathogenesis of EAE by promoting CNS inflammation. Targeting platelets may therefore represent an important new therapeutic approach for MS treatment. (Circ Res. 2012;110:1202-1210.) C1 [Langer, Harald F.; Schleicher, Rebecca] Univ Tubingen, Med Klin Kardiol & Kreislauferkrankungen 3, D-72076 Tubingen, Germany. [Langer, Harald F.; Choi, Eun Young; Chung, Kyoung-Jin; Bhatia, Sumeena; Kruhlak, Michael J.; Chavakis, Triantafyllos] NCI, Expt Immunol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Choi, Eun Young; Chung, Kyoung-Jin; Chatzigeorgiou, Antonios; Chavakis, Triantafyllos] Univ Dresden, Dept Internal Med 3, Div Vasc Inflammat Diabet & Kidney, Dresden, Germany. [Choi, Eun Young; Chung, Kyoung-Jin; Chatzigeorgiou, Antonios; Chavakis, Triantafyllos] Univ Dresden, Inst Physiol, Dresden, Germany. [Choi, Eun Young] Univ Ulsan, Grad Sch, Dept Med, Seoul, South Korea. [Zhou, Hong; Wong, Connie; Kubes, Paul] Univ Calgary, Fac Med, Dept Physiol & Biophys, Immunol Res Grp, Calgary, AB, Canada. [Tang, Zhongshu; Li, Xuri] NEI, NIH, Rockville, MD USA. [Goebel, Kerstin; Meuth, Sven G.] Univ Munster, Neurol Clin, Inst Physiol, D-4400 Munster, Germany. [Bdeir, Khalil; Qu, Hongchang; Lambris, John D.] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA. [Rose, John W.; Burns, James B.; Hill, Kenneth E.] VASLCHCS, Neurovirol Res Lab, Salt Lake City, UT USA. [Zhang, Yongqing; Lehrmann, Elin; Becker, Kevin G.] NIA, Gene Express & Genom Unit, NIH, Baltimore, MD 21224 USA. [Wang, Yunmei; Simon, Daniel I.] Case Western Reserve Univ, Sch Med, Univ Hosp Harrington McLaughlin Heart & Vasc Inst, Cleveland, OH USA. [Wang, Yunmei; Simon, Daniel I.] Case Western Reserve Univ, Sch Med, Case Cardiovasc Ctr, Cleveland, OH USA. [Nieswandt, Bernhard] Univ Wurzburg, DFG Res Ctr Expt Biomed, Chair Vasc Med, D-97070 Wurzburg, Germany. [Nieswandt, Bernhard] Univ Wurzburg, DFG Res Ctr Expt Biomed, Rudolf Virchow Ctr, D-97070 Wurzburg, Germany. RP Langer, HF (reprint author), Univ Tubingen, Med Klin Kardiol & Kreislauferkrankungen 3, Otfried Muellerstr 10, D-72076 Tubingen, Germany. EM Harald.Langer@med.uni-tuebingen.de OI Lehrmann, Elin/0000-0002-9869-9475; Wong, Connie/0000-0002-9020-1847; Lambris, John/0000-0002-9370-5776; Becker, Kevin/0000-0002-6794-6656 FU National Institutes of Health [HL57506, HL085816, HL073852]; National Cancer Institute; National Institute on Aging; National Multiple Sclerosis Society [RG3411B4/1]; German Research Foundation; Novartis Foundation for Therapeutic Research; IZKF of the University of Tubingen [1868-0-0]; Tuebingen Platelet Investigative Consortium (TuePIC); Clinical Research Unit (KFO) [274]; Volkswagen Foundation FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute (T.C.), and National Institute on Aging (K.G.B.), the National Institutes of Health grants HL57506, HL085816, and HL073852 (D.I.S.), the National Multiple Sclerosis Society grant RG3411B4/1 (J.W.R.), and the German Research Foundation and the Novartis Foundation for Therapeutic Research (T.C.). H.F.L. is supported by the IZKF program of the University of Tubingen (1868-0-0), the Tuebingen Platelet Investigative Consortium (TuePIC), which is funded by the Clinical Research Unit (KFO 274) of the German Research Foundation and the Volkswagen Foundation (Lichtenberg program). NR 60 TC 66 Z9 66 U1 1 U2 9 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7330 J9 CIRC RES JI Circ.Res. PD APR 27 PY 2012 VL 110 IS 9 BP 1202 EP + DI 10.1161/CIRCRESAHA.111.256370 PG 35 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA 933ZI UT WOS:000303406200015 PM 22456181 ER PT J AU Bunting, SF Callen, E Kozak, ML Kim, JM Wong, N Lopez-Contreras, AJ Ludwig, T Baer, R Faryabi, RB Malhowski, A Chen, HT Fernandez-Capetillo, O D'Andrea, A Nussenzweig, A AF Bunting, Samuel F. Callen, Elsa Kozak, Marina L. Kim, Jung Min Wong, Nancy Lopez-Contreras, Andres J. Ludwig, Thomas Baer, Richard Faryabi, Robert B. Malhowski, Amy Chen, Hua-Tang Fernandez-Capetillo, Oscar D'Andrea, Alan Nussenzweig, Andre TI BRCA1 Functions Independently of Homologous Recombination in DNA Interstrand Crosslink Repair SO MOLECULAR CELL LA English DT Article ID STRAND-BREAK REPAIR; CLASS-SWITCH RECOMBINATION; FANCONI-ANEMIA PATHWAY; V(D)J RECOMBINATION; DAMAGE-RESPONSE; CELL-CYCLE; POLY(ADP-RIBOSE) POLYMERASE; TUMOR SUPPRESSION; DEFICIENT CELLS; 53BP1 AB Brca1 is required for DNA repair by homologous recombination (HR) and normal embryonic development. Here we report that deletion of the DNA damage response factor 53BP1 overcomes embryonic lethality in Brca1-nullizygous mice and rescues HR deficiency, as measured by hypersensitivity to polyADP-ribose polymerase (PARP) inhibition. However, Brca1,53BP1 double-deficient cells are hypersensitive to DNA interstrand crosslinks (ICLs), indicating that BRCA1 has an additional role in DNA crosslink repair that is distinct from HR. Disruption of the nonhomologous end-joining (NHEJ) factor, Ku, promotes DNA repair in Brca1-deficient cells; however deletion of either Ku or 53BP1 exacerbates genomic instability in cells lacking FANCD2, a mediator of the Fanconi anemia pathway for ICL repair. BRCA1 therefore has two separate roles in ICL repair that can be modulated by manipulating NHEJ, whereas FANCD2 provides a key activity that cannot be bypassed by ablation of 53BP1 or Ku. C1 [Bunting, Samuel F.; Callen, Elsa; Kozak, Marina L.; Wong, Nancy; Faryabi, Robert B.; Malhowski, Amy; Chen, Hua-Tang; Nussenzweig, Andre] NCI, Lab Genome Integr, NIH, Bethesda, MD 20892 USA. [Ludwig, Thomas; Baer, Richard] Columbia Univ, Med Ctr, Irving Canc Res Ctr, Inst Canc Genet, New York, NY 10032 USA. [Kim, Jung Min; D'Andrea, Alan] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Radiat Oncol, Boston, MA 02115 USA. [Lopez-Contreras, Andres J.; Fernandez-Capetillo, Oscar] Spanish Natl Canc Res Ctr, Genom Instabil Grp, Madrid, Spain. RP Nussenzweig, A (reprint author), NCI, Lab Genome Integr, NIH, 37 Convent Dr,Room 1108, Bethesda, MD 20892 USA. EM andre_nussenzweig@nih.gov RI Faryabi, Robert/H-3544-2015; Fernandez-Capetillo, Oscar/H-3508-2015; Ludwig, Thomas/Q-6484-2016; OI Faryabi, Robert/0000-0002-7931-2175; Fernandez-Capetillo, Oscar/0000-0002-2690-6885; Ludwig, Thomas/0000-0003-3461-2585; Lopez-Contreras, Andres Joaquin/0000-0002-5517-7327 FU NIH; National Cancer Institute; Center for Cancer Research; Department of Defense [BC102335, K99/R00, 1K99CA160574-01] FX We thank Laura Niedernhofer, Jeremy Daniel, and Elise Kohn for helpful discussions and suggestions; Mark O'Connor for Parpi; and Michael Eckhaus and Mark Bryant for assistance with histology. The work was supported by the Intramural Research Program of the NIH, the National Cancer Institute, and the Center for Cancer Research, and by a Department of Defense grant to A.N. (BC102335) and a K99/R00 grant (1K99CA160574-01) to S.B. Research was conducted in compliance with the Animal Welfare Act Regulations and other federal statutes relating to animals and experiments involving animals and adheres to the principles set forth in the Guide for Care and Use of Laboratory Animals, National Research Council, 1996. NR 56 TC 108 Z9 110 U1 3 U2 23 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell PD APR 27 PY 2012 VL 46 IS 2 BP 125 EP 135 DI 10.1016/j.molcel.2012.02.015 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 933MH UT WOS:000303365300004 PM 22445484 ER PT J AU Citrin, DE Hitchcock, YJ Chung, EJ Frandsen, J Urick, ME Shield, W Gaffney, D AF Citrin, Deborah E. Hitchcock, Ying J. Chung, Eun Joo Frandsen, Jonathan Urick, Mary Ellen Shield, William Gaffney, David TI Determination of cytokine protein levels in oral secretions in patients undergoing radiotherapy for head and neck malignancies SO RADIATION ONCOLOGY LA English DT Article DE Saliva; Cytokine; Radiation; Milliplex ID RADIATION PNEUMONITIS; MARKERS; RISK; BETA AB Background: Cytokines may be elevated in tumor and normal tissues following irradiation. Cytokine expression in these tissues may predict for toxicity or tumor control. The purpose of this pilot study was to determine the feasibility of measuring local salivary cytokine levels using buccal sponges in patients receiving chemo-radiation for head and neck malignancies. Patients and methods: 11 patients with epithelial malignancies of the head and neck were recruiting to this study. All patients received radiotherapy to the head and neck region with doses ranging between 60 - 67.5 Gy. Chemotherapy was delivered concurrently with radiation in all patients. Salivary samples were obtained from high dose and low dose regions prior to treatment and at three intervals during treatment for assessment of cytokine levels (IL-4, IL-6, IL-8, IL-10, EGF, MCP-1, TNF-alpha, and VEGF). Results: Cytokine levels were detectable in the salivary samples. Salivary cytokine levels of IL-4, IL-6, IL-8, EGF, MCP-1, TNF-alpha, and VEGF were higher in the high dose region compared to the low dose region at all time points (p < 0.05). A trend toward an increase in cytokine levels as radiation dose increased was observed for IL-6, IL-8, MCP-1, and TNF-alpha. Conclusion: Assessment of salivary cytokine levels may provide a novel method to follow local cytokine levels during radiotherapy and may provide a mechanism to study cytokine levels in a regional manner. C1 [Citrin, Deborah E.; Chung, Eun Joo; Urick, Mary Ellen; Shield, William] NCI, Radiat Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Hitchcock, Ying J.; Frandsen, Jonathan; Gaffney, David] Univ Utah, Dept Radiat Oncol, Huntsman Canc Inst, Salt Lake City, UT USA. RP Citrin, DE (reprint author), NCI, Radiat Oncol Branch, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA. EM citrind@mail.nih.gov FU Intramural Research Program of the NIH, NCI, CCR FX This research was supported in part by the Intramural Research Program of the NIH, NCI, CCR. NR 12 TC 12 Z9 12 U1 0 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1748-717X J9 RADIAT ONCOL JI Radiat. Oncol. PD APR 26 PY 2012 VL 7 AR 64 DI 10.1186/1748-717X-7-64 PG 6 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA 004CK UT WOS:000308657500001 PM 22537315 ER PT J AU Browne, SK Zaman, R Sampaio, EP Jutivorakool, K Rosen, LB Ding, L Pancholi, MJ Yang, LM Priel, DL Uzel, G Freeman, AF Hayes, CE Baxter, R Cohen, SH Holland, SM AF Browne, Sarah K. Zaman, Rifat Sampaio, Elizabeth P. Jutivorakool, Kamonwan Rosen, Lindsey B. Ding, Li Pancholi, Minjal J. Yang, Lauren M. Priel, Debra Long Uzel, Gulbu Freeman, Alexandra F. Hayes, Carlton E. Baxter, Roger Cohen, Stuart H. Holland, Steven M. TI Anti-CD20 (rituximab) therapy for anti-IFN-gamma autoantibody-associated nontuberculous mycobacterial infection SO BLOOD LA English DT Article ID INTERFERON-GAMMA; RHEUMATOID-ARTHRITIS; PEMPHIGUS-VULGARIS; MYASTHENIA-GRAVIS; AVIUM COMPLEX; PATIENT; INTERLEUKIN-12; AUTOIMMUNITY; RECURRENT; LYMPHOMA AB Patients with anti-IFN-gamma autoantibodies have impaired IFN-gamma signaling, leading to severe disseminated infections with intracellular pathogens, especially nontuberculous mycobacteria. Disease may be severe and progressive, despite aggressive treatment. To address the underlying pathogenic IFN-gamma autoantibodies we used the therapeutic monoclonal rituximab (anti-CD20) to target patient B cells. All subjects received between 8 and 12 doses of rituximab within the first year to maintain disease remission. Subsequent doses were given for relapsed infection. We report 4 patients with refractory disease treated with rituximab who had clinical and laboratory evidence of therapeutic response as determined by clearance of infection, resolution of inflammation, reduction of anti-IFN-gamma autoantibody levels, and improved IFN-gamma signaling. (Blood. 2012;119(17):3933-3939) C1 [Browne, Sarah K.; Zaman, Rifat; Sampaio, Elizabeth P.; Jutivorakool, Kamonwan; Rosen, Lindsey B.; Ding, Li; Pancholi, Minjal J.; Yang, Lauren M.; Uzel, Gulbu; Freeman, Alexandra F.; Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Sampaio, Elizabeth P.] Fiocruz MS, Inst Oswaldo Cruz, Leprosy Lab, BR-21045900 Rio De Janeiro, Brazil. [Jutivorakool, Kamonwan] Chulalongkorn Univ, Fac Med, Dept Med, Bangkok, Thailand. [Jutivorakool, Kamonwan] King Chulalongkorn Mem Hosp, Thai Red Cross Soc, Bangkok, Thailand. [Yang, Lauren M.] Washington Univ, Sch Med, St Louis, MO USA. [Priel, Debra Long] SAIC Frederick Inc, Clin Serv Program, Natl Canc Inst Frederick, Frederick, MD USA. [Hayes, Carlton E.] Jackson Clin, Jackson, TN USA. [Baxter, Roger] So Calif Permanente Med Grp, Oakland, CA USA. [Cohen, Stuart H.] Univ Calif Davis, Dept Epidemiol & Infect Control, Davis, CA 95616 USA. RP Browne, SK (reprint author), CRC B3-4141,MSC 1684, Bethesda, MD 20892 USA. EM brownesa@niaid.nih.gov FU Division of Intramural Research; National Institute of Allergy and Infectious Diseases; National Institutes of Health [HHSN261200800001E]; National Cancer Institute FX This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, and the National Cancer Institute, National Institutes of Health (contract HHSN261200800001E). NR 27 TC 42 Z9 42 U1 0 U2 1 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 26 PY 2012 VL 119 IS 17 BP 3933 EP 3939 DI 10.1182/blood-2011-12-395707 PG 7 WC Hematology SC Hematology GA 959AU UT WOS:000305282900014 PM 22403254 ER PT J AU Jun, HS Cheung, YY Lee, YM Mansfield, BC Chou, JY AF Jun, Hyun Sik Cheung, Yuk Yin Lee, Young Mok Mansfield, Brian C. Chou, Janice Y. TI Glucose-6-phosphatase-beta, implicated in a congenital neutropenia syndrome, is essential for macrophage energy homeostasis and functionality SO BLOOD LA English DT Article ID GLYCOGEN-STORAGE-DISEASE; ENDOPLASMIC-RETICULUM; MESOTHELIAL CELLS; NADPH OXIDASE; PREGNANCY; EXPRESSION; ACTIVATION; PROTEIN; RECRUITMENT; MUTATIONS AB Glucose-6-phosphatase-beta (G6Pase-beta or G6PC3) deficiency, also known as severe congenital neutropenia syndrome 4, is characterized not only by neutropenia but also by impaired neutrophil energy homeostasis and functionality. We now show the syndrome is also associated with macrophage dysfunction, with murine G6pc3(-/-) macrophages having impairments in their respiratory burst, chemotaxis, calcium flux, and phagocytic activities. Consistent with a glucose-6-phosphate (G6P) metabolism deficiency, G6pc3(-/-) macrophages also have a lower glucose uptake and lower levels of G6P, lactate, and ATP than wild-type macrophages. Furthermore, the expression of NADPH oxidase subunits and membrane translocation of p47(phox) are down-regulated, and G6pc3(-/-) macrophages exhibit repressed trafficking in vivo both during an inflammatory response and in pregnancy. During pregnancy, the absence of G6Pase-beta activity also leads to impaired energy homeostasis in the uterus and reduced fertility of G6pc3(-/-) mothers. Together these results show that immune deficiencies in this congenital neutropenia syndrome extend beyond neutrophil dysfunction. (Blood. 2012; 119(17):4047-4055) C1 [Jun, Hyun Sik; Cheung, Yuk Yin; Lee, Young Mok; Mansfield, Brian C.; Chou, Janice Y.] Eunice Kennedy Shriver Natl Inst Child Hlth Human, Sect Cellular Differentiat, Program Dev Endocrinol & Genet, NIH, Bethesda, MD 20892 USA. RP Chou, JY (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth Human, Sect Cellular Differentiat, Program Dev Endocrinol & Genet, NIH, 9000 Rockville Pike,Bldg 10,Rm 9D42, Bethesda, MD 20892 USA. EM chouja@mail.nih.gov RI Jun, Hyun Sik/C-6799-2013 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health FX This work was supported by the Intramural Research Programs of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health. NR 46 TC 12 Z9 12 U1 2 U2 3 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 26 PY 2012 VL 119 IS 17 BP 4047 EP 4055 DI 10.1182/blood-2011-09-377820 PG 9 WC Hematology SC Hematology GA 959AU UT WOS:000305282900027 PM 22246029 ER PT J AU Glisic, S Jailwala, P AF Glisic, Sanja Jailwala, Parthav TI Interaction between Treg Apoptosis Pathways, Treg Function and HLA Risk Evolves during Type 1 Diabetes Pathogenesis SO PLOS ONE LA English DT Article ID ENDOPLASMIC-RETICULUM STRESS; MHC CLASS-II; INDUCED CELL-DEATH; HUMAN T-CELLS; POLYMERASE CHAIN-REACTION; NF-KAPPA-B; OXIDATIVE STRESS; HUMAN NEUTROPHILS; HIGH-RESOLUTION; MESSENGER-RNA AB We have previously reported increased apoptosis of regulatory T cells (Tregs) in recent-onset Type 1 Diabetes subjects (RO T1D) in the honeymoon phase and in multiple autoantibody-positive (Ab+) subjects, some of which are developing T1D. We have also reported that increased Treg apoptosis was associated with High HLA risk and that it subsided with cessation of honeymoon period. In this report, we present results generated using genetics, genomics, functional cell-based assays and flow cytometry to assess cellular changes at the T-cell level during T1D pathogenesis. We measured ex vivo Treg apoptosis and Treg function, surface markers expression, expression of HLA class II genes, the influence of HLA risk on Treg apoptosis and function, and evaluated contribution of genes reported to be involved in the apoptosis process. This integrated comprehensive approach uncovered important information that can serve as a basis for future studies aimed to modulate Treg cell responsiveness to apoptotic signals in autoimmunity. For example, T1D will progress in those subjects where increased Treg apoptosis is accompanied with decreased Treg function. Furthermore, Tregs from High HLA risk healthy controls had increased Treg apoptosis levels and overexpressed FADD but not Fas/FasL. Tregs from RO T1D subjects in the honeymoon phase were primarily dying through withdrawal of growth hormones with contribution of oxidative stress, mitochondrial apoptotic pathways, and employment of TNF-receptor family members. Ab+ subjects, however, expressed high inflammation level, which probably contributed to Treg apoptosis, although other apoptotic pathways were also activated: withdrawal of growth hormones, oxidative stress, mitochondrial apoptosis and Fas/FasL apoptotic pathways. The value of these results lie in potentially different preventive treatment subjects would receive depending on disease progression stage when treated. C1 [Glisic, Sanja] Med Coll Wisconsin, Childrens Hosp Wisconsin, Human & Mol Genet Ctr, Dept Pediat,Max McGee Natl Res Ctr Juvenile Diabe, Milwaukee, WI 53226 USA. [Jailwala, Parthav] NCI Frederick, SAIC Frederick, Adv Biomed Comp Ctr, Bethesda, MD USA. RP Glisic, S (reprint author), Med Coll Wisconsin, Childrens Hosp Wisconsin, Human & Mol Genet Ctr, Dept Pediat,Max McGee Natl Res Ctr Juvenile Diabe, Milwaukee, WI 53226 USA. EM sglisic@mcw.edu FU Research Affairs Committee [9304653]; Max McGee National Research Center for Juvenile Diabetes; Human Molecular Genetics Center at Medical College of Wisconsin; Children's Research Institute of Wisconsin FX This study was supported by Research Affairs Committee #9304653 and through Max McGee National Research Center for Juvenile Diabetes, Human Molecular Genetics Center at Medical College of Wisconsin and Children's Research Institute of Wisconsin. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 76 TC 2 Z9 2 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 26 PY 2012 VL 7 IS 4 AR e36040 DI 10.1371/journal.pone.0036040 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WQ UT WOS:000305349100063 PM 22563437 ER PT J AU Walsh, RK Bradley, C Apperson, CS Gould, F AF Walsh, Rachael K. Bradley, Caitlin Apperson, Charles S. Gould, Fred TI An Experimental Field Study of Delayed Density Dependence in Natural Populations of Aedes albopictus SO PLOS ONE LA English DT Article ID TREE-HOLE ECOSYSTEMS; FEEDING-BEHAVIOR; INTERSPECIFIC COMPETITION; CONTAINER MOSQUITOS; DYNAMICS; CULICIDAE; AEGYPTI; DIPTERA; DETRITUS; SURVIVAL AB Aedes albopictus, a species known to transmit dengue and chikungunya viruses, is primarily a container-inhabiting mosquito. The potential for pathogen transmission by Ae. albopictus has increased our need to understand its ecology and population dynamics. Two parameters that we know little about are the impact of direct density-dependence and delayed density-dependence in the larval stage. The present study uses a manipulative experimental design, under field conditions, to understand the impact of delayed density dependence in a natural population of Ae. albopictus in Raleigh, North Carolina. Twenty liter buckets, divided in half prior to experimentation, placed in the field accumulated rainwater and detritus, providing oviposition and larval production sites for natural populations of Ae. albopictus. Two treatments, a larvae present and larvae absent treatment, were produced in each bucket. After five weeks all larvae were removed from both treatments and the buckets were covered with fine mesh cloth. Equal numbers of first instars were added to both treatments in every bucket. Pupae were collected daily and adults were frozen as they emerged. We found a significant impact of delayed density-dependence on larval survival, development time and adult body size in containers with high larval densities. Our results indicate that delayed density-dependence will have negative impacts on the mosquito population when larval densities are high enough to deplete accessible nutrients faster than the rate of natural food accumulation. C1 [Walsh, Rachael K.; Apperson, Charles S.; Gould, Fred] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Bradley, Caitlin] Univ N Carolina, Chapel Hill, NC USA. [Gould, Fred] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. RP Walsh, RK (reprint author), N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. EM rskatz@ncsu.edu FU National Institutes of Health (US) (NIH) [R01-AI54954-0IA2]; Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative FX This work was supported by National Institutes of Health (US) (NIH) grant R01-AI54954-0IA2 and a grant to the Regents of the University of California from the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 5 Z9 5 U1 2 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 26 PY 2012 VL 7 IS 4 AR e35959 DI 10.1371/journal.pone.0035959 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WQ UT WOS:000305349100054 PM 22563428 ER PT J AU Spivey, TL De Giorgi, V Zhao, YD Bedognetti, D Pos, Z Liu, QZ Tomei, S Ascierto, ML Uccellini, L Reinboth, J Chouchane, L Stroncek, DF Wang, E Marincola, FM AF Spivey, Tara L. De Giorgi, Valeria Zhao, Yingdong Bedognetti, Davide Pos, Zoltan Liu, Qiuzhen Tomei, Sara Ascierto, Maria Libera Uccellini, Lorenzo Reinboth, Jennifer Chouchane, Lotfi Stroncek, David F. Wang, Ena Marincola, Francesco M. TI The stable traits of melanoma genetics: an alternate approach to target discovery SO BMC GENOMICS LA English DT Article DE Melanoma; Melanoma genetics; Cancer; Tumor microenvironment ID COMPARATIVE GENOMIC HYBRIDIZATION; COLORECTAL-CANCER; PROGNOSTIC RELEVANCE; ACQUIRED-RESISTANCE; MALIGNANT-MELANOMA; ANTIGEN EXPRESSION; TISSUE MICROARRAY; CELL CARCINOMA; T-CELLS; TUMORS AB Background: The weight that gene copy number plays in transcription remains controversial; although in specific cases gene expression correlates with copy number, the relationship cannot be inferred at the global level. We hypothesized that genes steadily expressed by 15 melanoma cell lines (CMs) and their parental tissues (TMs) should be critical for oncogenesis and their expression most frequently influenced by their respective copy number. Results: Functional interpretation of 3,030 transcripts concordantly expressed (Pearson's correlation coefficient p-value < 0.05) by CMs and TMs confirmed an enrichment of functions crucial to oncogenesis. Among them, 968 were expressed according to the transcriptional efficiency predicted by copy number analysis (Pearson's correlation coefficient p-value < 0.05). We named these genes, "genomic delegates" as they represent at the transcriptional level the genetic footprint of individual cancers. We then tested whether the genes could categorize 112 melanoma metastases. Two divergent phenotypes were observed: one with prevalent expression of cancer testis antigens, enhanced cyclin activity, WNT signaling, and a Th17 immune phenotype (Class A). This phenotype expressed, therefore, transcripts previously associated to more aggressive cancer. The second class (B) prevalently expressed genes associated with melanoma signaling including MITF, melanoma differentiation antigens, and displayed a Th1 immune phenotype associated with better prognosis and likelihood to respond to immunotherapy. An intermediate third class (C) was further identified. The three phenotypes were confirmed by unsupervised principal component analysis. Conclusions: This study suggests that clinically relevant phenotypes of melanoma can be retraced to stable oncogenic properties of cancer cells linked to their genetic back bone, and offers a roadmap for uncovering novel targets for tailored anti-cancer therapy. C1 [Spivey, Tara L.; De Giorgi, Valeria; Bedognetti, Davide; Liu, Qiuzhen; Tomei, Sara; Ascierto, Maria Libera; Uccellini, Lorenzo; Reinboth, Jennifer; Wang, Ena; Marincola, Francesco M.] NIH, Infect Dis & Immunogenet Sect IDIS, Dept Transfus Med, Ctr Clin, Bethesda, MD 20892 USA. [Spivey, Tara L.] NIH, Clin Res Training Program CRTP, Bethesda, MD 20892 USA. [Spivey, Tara L.] Rush Univ, Med Ctr, Rush Med Coll, Chicago, IL 60612 USA. [Zhao, Yingdong] NCI, Biometr Res Branch, Div Canc Treatment & Diag, NIH, Bethesda, MD 20892 USA. [Bedognetti, Davide; Ascierto, Maria Libera] Univ Genoa, Dept Internal Med DiMI, I-16132 Genoa, Italy. [Bedognetti, Davide] Natl Canc Res Inst Genoa, Genoa, Italy. [Bedognetti, Davide] Dept Oncol Biol & Genet, Genoa, Italy. [Pos, Zoltan] Semmelweis Univ, Dept Genet Cell & Immunobiol, H-1089 Budapest, Hungary. [Tomei, Sara] Univ Pisa, Dept Oncol, Pisa, Italy. [Ascierto, Maria Libera] Univ Genoa, Ctr Excellence Biomed Res CEBR, Genoa, Italy. [Chouchane, Lotfi] Weill Cornell Med Coll Qatar, Doha, Qatar. [Stroncek, David F.] NIH, Cell Proc Sect, Dept Transfus Med, Ctr Clin, Bethesda, MD 20892 USA. [Uccellini, Lorenzo] Univ Milan, Inst Infect & Trop Dis, L Sacco Hosp, Milan, Italy. [Reinboth, Jennifer] Genelux Corp, San Diego Sci Ctr, San Diego, CA USA. [Reinboth, Jennifer] Univ Wurzburg, Dept Biochem, Bioctr, D-97074 Wurzburg, Germany. [Marincola, Francesco M.] NIH, Infect Dis & Immunogenet Sect IDIS, Dept Transfus Med, Ctr Human Immunol CHI, Bethesda, MD 20892 USA. [Spivey, Tara L.; De Giorgi, Valeria; Bedognetti, Davide; Liu, Qiuzhen; Tomei, Sara; Ascierto, Maria Libera; Uccellini, Lorenzo; Reinboth, Jennifer; Wang, Ena; Marincola, Francesco M.] NIH, Infect Dis & Immunogenet Sect IDIS, Dept Transfus Med, Trans NIH Ctr Human Immunol CHI, Bethesda, MD 20892 USA. RP De Giorgi, V (reprint author), NIH, Infect Dis & Immunogenet Sect IDIS, Dept Transfus Med, Ctr Clin, Bldg 10, Bethesda, MD 20892 USA. EM degiorgiv@mail.nih.gov; FMarincola@mail.cc.nih.gov RI Pos, Zoltan/C-3623-2014; De Giorgi, Valeria/D-4582-2017; OI Pos, Zoltan/0000-0002-2574-7616; Bedognetti, Davide/0000-0002-5857-773X FU NIH; Pfizer Inc. FX Tara Spivey's research fellowship was made possible through the Clinical Research Training Program, a public-private partnership supported jointly by the NIH and Pfizer Inc. (via a grant to the foundation for NIH from Pfizer Inc.). NR 46 TC 20 Z9 20 U1 0 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 26 PY 2012 VL 13 AR 156 DI 10.1186/1471-2164-13-156 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 949HR UT WOS:000304567800001 PM 22537248 ER PT J AU Xu, Y Ma, BY Nussinov, R AF Xu, Yu Ma, Buyong Nussinov, Ruth TI Structural and Functional Consequences of Phosphate-Arsenate Substitutions in Selected Nucleotides: DNA, RNA, and ATP SO JOURNAL OF PHYSICAL CHEMISTRY B LA English DT Article ID MOLECULAR-DYNAMICS SIMULATIONS; CONFORMATIONAL PROPERTIES; POTENTIAL FUNCTIONS; LIQUID WATER; FORCE-FIELD; RIBOSOME; PHOSPHORUS; BACTERIUM; PROTEIN; GROW AB A recent finding of a bacterial strain (GFAJ-1) that can rely on arsenic instead of phosphorus raised the questions of if and how arsenate can replace phosphate in biomolecules that are essential to sustain cell life. Apart from questions related to chemical stability, there are those of the structural and functional consequences of phosphate-arsenate substitutions in vital nucleotides in GFAJ1-like cells. In this study we selected three types of molecules (ATP/ADP as energy source and replication regulation; DNA protein complexes for DNA replication and transcription initiation; and a tRNA protein complex and ribosome for protein synthesis) to computationally probe if arsenate nucleotides can retain the structural and functional features of phosphate nucleotides. Hydrolysis of adenosine triarsenate provides 2-3 kcal/mol less energy than ATP hydrolysis. Arsenate DNA/RNA interacts with proteins slightly less strongly than phosphate DNA/RNA, mainly due to the weaker electrostatic interactions of arsenate. We observed that the weaker arsenate RNA protein interactions may hamper rRNA assembly into a functional ribosome. We further compared the experimental EXAFS spectra of the arsenic bacteria with theoretical EXAFS spectra for arsenate DNA and rRNA. Our results demonstrate that while it is possible that dried GFAJ-1 cells contain linear arsenate DNA, the arsenate 70S ribosome does not contribute to the main arsenate depository in the GFAJ-1 cell. Our study indicates that evolution has optimized the inter-relationship between proteins and DNA/RNA, which requires overall changes at the molecular and systems biology levels when replacing phosphate by arsenate. C1 [Ma, Buyong] NCI, Basic Sci Program, SAIC Frederick Inc, Ctr Canc Res Nanobiol Program,NIH, Frederick, MD 21702 USA. [Xu, Yu; Nussinov, Ruth] Minzu Univ China, Inst Chinese Minor Tradit Med, Beijing 100081, Peoples R China. [Nussinov, Ruth] Tel Aviv Univ, Sackler Sch Med, Sackler Inst Mol Med, Dept Human Genet & Mol Med, IL-69978 Tel Aviv, Israel. RP Ma, BY (reprint author), NCI, Basic Sci Program, SAIC Frederick Inc, Ctr Canc Res Nanobiol Program,NIH, Frederick, MD 21702 USA. EM mabuyong@mail.nih.gov; ruthnu@helix.nih.gov RI Ma, Buyong/F-9491-2011 OI Ma, Buyong/0000-0002-7383-719X FU National Cancer Institute, National Institutes of Health [HHSN261200800001E]; China Scholarship Council; NIH, NCI, Center for Cancer Research FX This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under contract number HHSN261200800001E. Y.X. thanks the China Scholarship Council [2009]3009. This research was supported (in part) by the Intramural Research Program of the NIH, NCI, Center for Cancer Research. All simulations were performed using the high-performance computational facilities of the Biowulf PC/Limix cluster at the NIH, Bethesda, MD (http://biowulf.nih.gov). NR 56 TC 8 Z9 8 U1 1 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1520-6106 J9 J PHYS CHEM B JI J. Phys. Chem. B PD APR 26 PY 2012 VL 116 IS 16 BP 4801 EP 4811 DI 10.1021/jp300307u PG 11 WC Chemistry, Physical SC Chemistry GA 930VX UT WOS:000303173800009 PM 22480264 ER PT J AU Montesarchio, V Grimaldi, AM Fox, BA Rea, A Marincola, FM Ascierto, PA AF Montesarchio, Vincenzo Grimaldi, Antonio Maria Fox, Bernard A. Rea, Antonio Marincola, Francesco M. Ascierto, Paolo A. TI Lean oncology: a new model for oncologists SO JOURNAL OF TRANSLATIONAL MEDICINE LA English DT Editorial Material AB The history of the term Lean is relatively recent and originates from the Toyota Production System (TPS). The term "Lean" means "thin", which refers to a mental process, operational, productive, no-frills, quick but not hasty, consequential to the previous event. The Lean process flows seamlessly into the result, eliminates unnecessary complications to the effect, prevents unnecessary equipment processes. The idea is to 'do more with less', like using the (few) available resources in the most productive way possible, through the elimination of all types of waste that inevitably accompanies every stage of a production process. Lean management is primarily a management philosophy, a system of values and behaviors that goes beyond the mere application of the instrument and that, once internalized, will form the nucleus of the corporate culture. "Lean Oncology" is a term coined to identify a methodology of care and treatment to cancer patients, consisting on process simplification, streamlining of the organizational and routes of drug treatment, detection and elimination of waste. Its main objective is the centrality of the patient. C1 [Grimaldi, Antonio Maria; Ascierto, Paolo A.] Ist Nazl Studio & Cura Tumori Fdn G Pascale, Unit Med Oncol & Innovat Therapy, I-80131 Naples, Italy. [Montesarchio, Vincenzo; Rea, Antonio] Osped D Cotugno AORN Colli, Unit Med Oncol, I-80131 Naples, Italy. [Fox, Bernard A.] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA. [Fox, Bernard A.] Oregon Hlth & Sci Univ, Providence Portland Med Ctr, Robert W Franz Res Ctr, Earle A Chiles Res Inst, Portland, OR 97201 USA. [Marincola, Francesco M.] Clin Ctr & Trans NIH, Infect Dis & Immunogenet Sect, Bethesda, MD USA. RP Ascierto, PA (reprint author), Ist Nazl Studio & Cura Tumori Fdn G Pascale, Unit Med Oncol & Innovat Therapy, Via Mariano Semmola, I-80131 Naples, Italy. EM paolo.ascierto@gmail.com FU Intramural NIH HHS [Z99 CL999999] NR 0 TC 2 Z9 3 U1 0 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5876 J9 J TRANSL MED JI J. Transl. Med. PD APR 25 PY 2012 VL 10 AR 74 DI 10.1186/1479-5876-10-74 PG 3 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 033LA UT WOS:000310796400001 PM 22533796 ER PT J AU Garzon-Martinez, GA Zhu, ZI Landsman, D Barrero, LS Marino-Ramirez, L AF Garzon-Martinez, Gina A. Zhu, Z. Iris Landsman, David Barrero, Luz S. Marino-Ramirez, Leonardo TI The Physalis peruviana leaf transcriptome: assembly, annotation and gene model prediction SO BMC GENOMICS LA English DT Article DE P. peruviana; Solanaceae; ESTs; Functional annotation; Gene model; Phylogenetics ID NORMALIZED CDNA LIBRARY; HIGH-THROUGHPUT; DISEASE RESISTANCE; PLANT DEFENSE; EST-SSRS; SOLANACEAE; DISCOVERY; IDENTIFICATION; GENERATION; EVOLUTION AB Background: Physalis peruviana commonly known as Cape gooseberry is a member of the Solanaceae family that has an increasing popularity due to its nutritional and medicinal values. A broad range of genomic tools is available for other Solanaceae, including tomato and potato. However, limited genomic resources are currently available for Cape gooseberry. Results: We report the generation of a total of 652,614 P. peruviana Expressed Sequence Tags (ESTs), using 454 GS FLX Titanium technology. ESTs, with an average length of 371 bp, were obtained from a normalized leaf cDNA library prepared using a Colombian commercial variety. De novo assembling was performed to generate a collection of 24,014 isotigs and 110,921 singletons, with an average length of 1,638 bp and 354 bp, respectively. Functional annotation was performed using NCBI's BLAST tools and Blast2GO, which identified putative functions for 21,191 assembled sequences, including gene families involved in all the major biological processes and molecular functions as well as defense response and amino acid metabolism pathways. Gene model predictions in P. peruviana were obtained by using the genomes of Solanum lycopersicum (tomato) and Solanum tuberosum (potato). We predict 9,436 P. peruviana sequences with multiple-exon models and conserved intron positions with respect to the potato and tomato genomes. Additionally, to study species diversity we developed 5,971 SSR markers from assembled ESTs. Conclusions: We present the first comprehensive analysis of the Physalis peruviana leaf transcriptome, which will provide valuable resources for development of genetic tools in the species. Assembled transcripts with gene models could serve as potential candidates for marker discovery with a variety of applications including: functional diversity, conservation and improvement to increase productivity and fruit quality. P. peruviana was estimated to be phylogenetically branched out before the divergence of five other Solanaceae family members, S. lycopersicum, S. tuberosum, Capsicum spp, S. melongena and Petunia spp. C1 [Garzon-Martinez, Gina A.; Barrero, Luz S.; Marino-Ramirez, Leonardo] Colombian Corp Agr Res CORPOICA, Plant Mol Genet Lab, Ctr Biotechnol & Bioind, Bogota, Colombia. [Zhu, Z. Iris; Landsman, David; Marino-Ramirez, Leonardo] Natl Lib Med, Computat Biol Branch, Natl Ctr Biotechnol Informat, Bethesda, MD USA. [Barrero, Luz S.; Marino-Ramirez, Leonardo] PanAmer Bioinformat Inst, Magdalena, Colombia. RP Marino-Ramirez, L (reprint author), Colombian Corp Agr Res CORPOICA, Plant Mol Genet Lab, Ctr Biotechnol & Bioind, Bogota, Colombia. EM marino@ncbi.nlm.nih.gov RI Marino-Ramirez, Leonardo/I-5759-2013; OI Marino-Ramirez, Leonardo/0000-0002-5716-8512; Landsman, David/0000-0002-9819-6675 FU Colombian Ministry of Agriculture [054/08072-2008 L4787-3281, 054/08190-2008 L7922-3322]; Colciencias "Joven Investigador" Fellowship; National Institutes of Health, National Library of Medicine; National Center for Biotechnology Information FX Support for this research was provided by a grant from the Colombian Ministry of Agriculture Contract Nos. 054/08072-2008 L4787-3281 to Luz Stella Barrero and 054/08190-2008 L7922-3322 to Victor Manuel Nunez Zarantes. Gina Garzon-Martinez was supported by a Colciencias "Joven Investigador" Fellowship during 2010. Leonardo Marino-Ramirez expresses his deepest gratitude to his friend and colleague Dr. Alba Marina Cotes Prado for all the advice and support she gave him to conduct this project. This research was supported in part by the Intramural Research Program of the National Institutes of Health, National Library of Medicine, and National Center for Biotechnology Information. NR 56 TC 18 Z9 19 U1 2 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 25 PY 2012 VL 13 AR 151 DI 10.1186/1471-2164-13-151 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 979WZ UT WOS:000306855100001 PM 22533342 ER PT J AU Major, JM Kiruthu, C Weinstein, SJ Horst, RL Snyder, K Virtamo, J Albanes, D AF Major, Jacqueline M. Kiruthu, Christine Weinstein, Stephanie J. Horst, Ronald L. Snyder, Kirk Virtamo, Jarmo Albanes, Demetrius TI Pre-Diagnostic Circulating Vitamin D and Risk of Melanoma in Men SO PLOS ONE LA English DT Article ID MALIGNANT-MELANOMA; SUN EXPOSURE; CANCER AB Purpose: Various studies have examined the association between serum vitamin D levels and different cancers; however, this is the first prospective study of this association with melanoma risk. The aim of this study is to investigate the association between serum vitamin D [25(OH)D] levels and melanoma in a cohort of older, middle-aged Finnish male smokers. Methods: We conducted a nested case-control study within the Alpha-Tocopherol Beta-Carotene Cancer Prevention (ATBC) Study. From the ATBC cohort, 368 subjects were chosen for our study; 92 participants that developed melanoma and 276 matched control subjects. At study baseline, lifestyle questionnaires and blood samples were collected. Serum 25(OH)D was modeled as three sets of categorical variables: clinically-defined categories, season-specific quartiles and season-adjusted residual quartiles. Conditional logistic regression was used to obtain odds ratios (ORs) and 95% confidence intervals (95% CIs) to estimate the association between circulating vitamin D and melanoma risk. Results: Overall no association of serum 25(OH)D and melanoma risk was observed. A decreased risk of developing melanoma was observed in the middle categories compared to the lowest category, albeit not significant. Conclusion: Results indicate no association between serum 25(OH)D levels and melanoma. Additional studies, including possibly consortium efforts, are needed to investigate the association between serum 25(OH)D levels and risk of melanoma in larger, more diverse study populations. C1 [Major, Jacqueline M.; Kiruthu, Christine; Weinstein, Stephanie J.; Albanes, Demetrius] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Horst, Ronald L.] Heartland Assays Inc, Ames, IA USA. [Snyder, Kirk] Informat Management Serv Inc, Silver Spring, MD USA. [Virtamo, Jarmo] Natl Inst Hlth & Welf, Dept Chron Dis Prevent, Helsinki, Finland. RP Major, JM (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. EM jacqueline.major@nih.gov RI Albanes, Demetrius/B-9749-2015 FU National Cancer Institute, NIH; US Public Health Service from the National Cancer Institute, NIH, DHHS [N01-CN-45165, N01-RC-45035, N01-RC-37004, HHSN261201000006C] FX The Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study is supported by the Intramural Research Program of the National Cancer Institute, NIH, and by US Public Health Service contracts N01-CN-45165, N01-RC-45035, N01-RC-37004, and HHSN261201000006C from the National Cancer Institute, NIH, DHHS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Ronald L. Horst is an employee of Heartland Assays, Inc., and Kirk Snyder is an analytic programmer employed by Information Management Services, Inc. These authors played a role in performing the experiments and analysis of the data. NR 28 TC 8 Z9 8 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 25 PY 2012 VL 7 IS 4 AR e35112 DI 10.1371/journal.pone.0035112 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VP UT WOS:000305345200026 PM 22558121 ER PT J AU Stroh, O Freichel, M Kretz, O Birnbaumer, L Hartmann, J Egger, V AF Stroh, Olga Freichel, Marc Kretz, Oliver Birnbaumer, Lutz Hartmann, Jana Egger, Veronica TI NMDA Receptor-Dependent Synaptic Activation of TRPC Channels in Olfactory Bulb Granule Cells SO JOURNAL OF NEUROSCIENCE LA English DT Article ID METABOTROPIC GLUTAMATE RECEPTORS; NONSELECTIVE CATIONIC CURRENT; LONG-LASTING DEPOLARIZATIONS; GABA RELEASE; MITRAL CELLS; DENDRODENDRITIC INHIBITION; RECIPROCAL SYNAPSES; POTENTIAL CHANNELS; PHOSPHOLIPASE-C; CALCIUM INFLUX AB Canonical transient receptor potential (TRPC) channels are widely expressed throughout the nervous system including the olfactory bulb where their function is largely unknown. Here, we describe their contribution to central synaptic processing at the reciprocal mitral and tufted cell-granule cell microcircuit, the most abundant synapse of the mammalian olfactory bulb. Suprathreshold activation of the synapse causes sodium action potentials in mouse granule cells and a subsequent long-lasting depolarization (LLD) linked to a global dendritic postsynaptic calcium signal recorded with two-photon laser-scanning microscopy. These signals are not observed after action potentials evoked by current injection in the same cells. The LLD persists in the presence of group I metabotropic glutamate receptor antagonists but is entirely absent from granule cells deficient for the NMDA receptor subunit NR1. Moreover, both depolarization and Ca2+ rise are sensitive to the blockade of NMDA receptors. The LLD and the accompanying Ca2+ rise are also absent in granule cells from mice deficient for both TRPC channel subtypes 1 and 4, whereas the deletion of either TRPC1 or TRPC4 results in only a partial reduction of the LLD. Recordings from mitral cells in the absence of both subunits reveal a reduction of asynchronous neurotransmitter release from the granule cells during recurrent inhibition. We conclude that TRPC1 and TRPC4 can be activated downstream of NMDA receptor activation and contribute to slow synaptic transmission in the olfactory bulb, including the calcium dynamics required for asynchronous release from the granule cell spine. C1 [Egger, Veronica] Univ Munich, Div Neurobiol, Dept Biol 2, D-82152 Martinsried, Germany. [Stroh, Olga; Egger, Veronica] Univ Munich, Inst Physiol, D-80336 Munich, Germany. [Freichel, Marc] Univ Saarland, Inst Pharmacol & Toxicol, D-66421 Homburg, Germany. [Kretz, Oliver] Univ Freiburg, Inst Anat & Cell Biol, Dept Neuroanat, D-79104 Freiburg, Germany. [Birnbaumer, Lutz] NIEHS, Res Triangle Pk, NC 27709 USA. [Hartmann, Jana] Tech Univ Munich, Inst Neurosci, D-80802 Munich, Germany. RP Egger, V (reprint author), Univ Munich, Div Neurobiol, Dept Biol 2, Grosshadernerstr 2, D-82152 Martinsried, Germany. EM v.egger@lmu.de RI Hartmann, Jana/C-1024-2008 FU Deutsche Forschungsgemeinschaft [SFB 391, SFB 870, SPP 1392, SFB 530]; NIH [Z01-ES-101684] FX This work was supported by Deutsche Forschungsgemeinschaft Grants SFB 391 (O.S.,V.E.), SFB 870 and SPP 1392 (V.E.), and SFB 530 (M.F.), and by NIH Intramural Research Program Grant Z01-ES-101684 (L.B.). We thank A. Schafer, T. Kuner, and P. Seeburg for the GluR NR1 2lox mice, V. Flockerzi for anti-TRPC1, anti-TRPC4, and anti-TRPC5 antibodies, B. Sutor and A. Konnerth for support, and I. Schneider, H. Jacobi, and R. Waberer for technical assistance. The authors declare no competing financial interests. NR 71 TC 25 Z9 26 U1 0 U2 6 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD APR 25 PY 2012 VL 32 IS 17 BP 5737 EP 5746 DI 10.1523/JNEUROSCI.3753-11.2012 PG 10 WC Neurosciences SC Neurosciences & Neurology GA 933VU UT WOS:000303393000005 PM 22539836 ER PT J AU Grishaev, A Ying, JF Bax, A AF Grishaev, Alexander Ying, Jinfa Bax, Ad TI Imino Hydrogen Positions in Nucleic Acids from Density Functional Theory Validated by NMR Residual Dipolar Couplings SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID GLOBAL STRUCTURE DETERMINATION; MOLECULAR-ORBITAL METHODS; HUMAN TELOMERASE RNA; X-RAY-SCATTERING; BASE-PAIRS; BASIS-SET; DYNAMICS; PROTEINS; BONDS AB Hydrogen atom positions of nucleotide bases in RNA structures solved by X-ray crystallography are commonly derived from heavy-atom coordinates by assuming idealized geometries. In particular, N1-H1 vectors in G and N3-H3 vectors in U are commonly positioned to coincide with the bisectors of their respective heavy-atom angles. We demonstrate that quantum-mechanical optimization of the hydrogen positions relative to their heavy-atom frames considerably improves the fit of experimental residual dipolar couplings to structural coordinates. The calculations indicate that deviations of the imino N-H vectors in RNA U and G bases result from H-bonding within the base pair and are dominated by the attractive interaction between the H atom and the electron density surrounding the H-bond-acceptor atom. DFT optimization of H atom positions is impractical in structural biology studies. We therefore have developed an empirical relation that predicts imino N-H vector orientations from the heavy-atom coordinates of the base pair. This relation agrees very closely with the DFT results, permitting its routine application in structural studies. C1 [Grishaev, Alexander; Ying, Jinfa; Bax, Ad] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Bax, A (reprint author), NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. EM bax@nih.gov FU National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (NIH); Office of the Director, NIH FX We thank Dr. Fernando Clemente of Gaussian, Inc. for helpful discussions and Dr. Yun-Xing Wang for the RiboA sample. This study utilized the high-performance computational capabilities of the Biowulf Linux cluster at the National Institutes of Health, Bethesda, Maryland (http://biowulf.nih.gov). This work was funded by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (NIH), and the Intramural AIDS-Targeted Antiviral Program of the Office of the Director, NIH. NR 26 TC 3 Z9 3 U1 1 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD APR 25 PY 2012 VL 134 IS 16 BP 6956 EP 6959 DI 10.1021/ja301775j PG 4 WC Chemistry, Multidisciplinary SC Chemistry GA 930LB UT WOS:000303139800018 PM 22489834 ER PT J AU Silverman, JL Smith, DG Rizzo, SJS Karras, MN Turner, SM Tolu, SS Bryce, DK Smith, DL Fonseca, K Ring, RH Crawley, JN AF Silverman, Jill L. Smith, Daniel G. Rizzo, Stacey J. Sukoff Karras, Michael N. Turner, Sarah M. Tolu, Seda S. Bryce, Dianne K. Smith, Deborah L. Fonseca, Kari Ring, Robert H. Crawley, Jacqueline N. TI Negative Allosteric Modulation of the mGluR5 Receptor Reduces Repetitive Behaviors and Rescues Social Deficits in Mouse Models of Autism SO SCIENCE TRANSLATIONAL MEDICINE LA English DT Article ID FRAGILE-X-SYNDROME; MUTANT MICE DISPLAY; BTBR-T+TF/J MICE; SPECTRUM DISORDER; CORPUS-CALLOSUM; RETT-SYNDROME; ULTRASONIC VOCALIZATIONS; TUBEROUS-SCLEROSIS; MENTAL-RETARDATION; LOW SOCIABILITY AB Neurodevelopmental disorders such as autism and fragile X syndrome were long thought to be medically un-treatable, on the assumption that brain dysfunctions were immutably hardwired before diagnosis. Recent revelations that many cases of autism are caused by mutations in genes that control the ongoing formation and maturation of synapses have challenged this dogma. Antagonists of metabotropic glutamate receptor subtype 5 (mGluR5), which modulate excitatory neurotransmission, are in clinical trials for fragile X syndrome, a major genetic cause of intellectual disabilities. About 30% of patients with fragile X syndrome meet the diagnostic criteria for autism. Reasoning by analogy, we considered the mGluR5 receptor as a potential target for intervention in autism. We used BTBR T+tf/J (BTBR) mice, an established model with robust behavioral phenotypes relevant to the three diagnostic behavioral symptoms of autism-unusual social interactions, impaired communication, and repetitive behaviors-to probe the efficacy of a selective negative allosteric modulator of the mGluR5 receptor, GRN-529. GRN-529 reduced repetitive behaviors in three cohorts of BTBR mice at doses that did not induce sedation in control assays of open field locomotion. In addition, the same nonsedating doses reduced the spontaneous stereotyped jumping that characterizes a second inbred strain of mice, C58/J. Further, GRN-529 partially reversed the striking lack of sociability in BTBR mice on some parameters of social approach and reciprocal social interactions. These findings raise the possibility that a single targeted pharmacological intervention may alleviate multiple diagnostic behavioral symptoms of autism. C1 [Silverman, Jill L.; Karras, Michael N.; Turner, Sarah M.; Tolu, Seda S.; Crawley, Jacqueline N.] NIMH, Lab Behav Neurosci, Bethesda, MD 20892 USA. [Smith, Daniel G.; Rizzo, Stacey J. Sukoff; Bryce, Dianne K.; Smith, Deborah L.; Fonseca, Kari; Ring, Robert H.] Pfizer Worldwide Res & Dev, Groton, CT 06340 USA. RP Crawley, JN (reprint author), NIMH, Lab Behav Neurosci, Bethesda, MD 20892 USA. EM crawleyj@mail.nih.gov RI Ring, Robert/M-1238-2016 OI Ring, Robert/0000-0002-7037-4409 FU NIMH; Pfizer Global Research FX This work was supported by the NIMH Intramural Research Program (J.L.S., M.N.K., S.M.T., S.S.T., and J.N.C.) and Pfizer Global Research (D.G.S., S.J.S.R., D.K.B., D.L.S., K.F., and R.H.R.). NR 103 TC 56 Z9 57 U1 4 U2 23 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 1946-6234 J9 SCI TRANSL MED JI Sci. Transl. Med. PD APR 25 PY 2012 VL 4 IS 131 AR 131ra51 DI 10.1126/scitranslmed.3003501 PG 9 WC Cell Biology; Medicine, Research & Experimental SC Cell Biology; Research & Experimental Medicine GA 933UM UT WOS:000303388900005 PM 22539775 ER PT J AU Perin, EC Willerson, JT Pepine, CJ Henry, TD Ellis, SG Zhao, DXM Silva, GV Lai, DJ Thomas, JD Kronenberg, MW Martin, AD Anderson, RD Traverse, JH Penn, MS Anwaruddin, S Hatzopoulos, AK Gee, AP Taylor, DA Cogle, CR Smith, D Westbrook, L Chen, J Handberg, E Olson, RE Geither, C Bowman, S Francescon, J Baraniuk, S Piller, LB Simpson, LM Loghin, C Aguilar, D Richman, S Zierold, C Bettencourt, J Sayre, SL Vojvodic, RW Skarlatos, SI Gordon, DJ Ebert, RF Kwak, M Moye, LA Simari, RD AF Perin, Emerson C. Willerson, James T. Pepine, Carl J. Henry, Timothy D. Ellis, Stephen G. Zhao, David X. M. Silva, Guilherme V. Lai, Dejian Thomas, James D. Kronenberg, Marvin W. Martin, A. Daniel Anderson, R. David Traverse, Jay H. Penn, Marc S. Anwaruddin, Saif Hatzopoulos, Antonis K. Gee, Adrian P. Taylor, Doris A. Cogle, Christopher R. Smith, Deirdre Westbrook, Lynette Chen, James Handberg, Eileen Olson, Rachel E. Geither, Carrie Bowman, Sherry Francescon, Judy Baraniuk, Sarah Piller, Linda B. Simpson, Lara M. Loghin, Catalin Aguilar, David Richman, Sara Zierold, Claudia Bettencourt, Judy Sayre, Shelly L. Vojvodic, Rachel W. Skarlatos, Sonia I. Gordon, David J. Ebert, Ray F. Kwak, Minjung Moye, Lemuel A. Simari, Robert D. CA Cardiovasc Cell Therapy Res Networ TI Effect of Transendocardial Delivery of Autologous Bone Marrow Mononuclear Cells on Functional Capacity, Left Ventricular Function, and Perfusion in Chronic Heart Failure The FOCUS-CCTRN Trial SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; ACUTE MYOCARDIAL-INFARCTION; CORONARY-ARTERY-DISEASE; RESEARCH NETWORK CCTRN; STEM-CELLS; PROGENITOR CELLS; TRANSCORONARY TRANSPLANTATION; INTRAMYOCARDIAL INJECTION; F-18 FLUORODEOXYGLUCOSE; METABOLIC-ACTIVITY AB Context Previous studies using autologous bone marrow mononuclear cells (BMCs) in patients with ischemic cardiomyopathy have demonstrated safety and suggested efficacy. Objective To determine if administration of BMCs through transendocardial injections improves myocardial perfusion, reduces left ventricular end-systolic volume (LVESV), or enhances maximal oxygen consumption in patients with coronary artery disease or LV dysfunction, and limiting heart failure or angina. Design, Setting, and Patients Aphase 2 randomized double-blind, placebo-controlled trial of symptomatic patients (New York Heart Association classification II-III or Canadian Cardiovascular Society classification II-IV) with a left ventricular ejection fraction of 45% or less, a perfusion defect by single-photon emission tomography (SPECT), and coronary artery disease not amenable to revascularization who were receiving maximal medical therapy at 5 National Heart, Lung, and Blood Institute-sponsored Cardiovascular Cell Therapy Research Network (CCTRN) sites between April 29, 2009, and April 18, 2011. Intervention Bone marrow aspiration (isolation of BMCs using a standardized automated system performed locally) and transendocardial injection of 100 million BMCs or placebo (ratio of 2 for BMC group to 1 for placebo group). Main Outcome Measures Co-primary end points assessed at 6 months: changes in LVESV assessed by echocardiography, maximal oxygen consumption, and reversibility on SPECT. Phenotypic and functional analyses of the cell product were performed by the CCTRN biorepository core laboratory. Results Of 153 patients who provided consent, a total of 92 (82 men; average age: 63 years) were randomized (n=61 in BMC group and n=31 in placebo group). Changes in LVESV index (-0.9 mL/m(2) [95% CI, -6.1 to 4.3]; P=.73), maximal oxygen consumption (1.0 [95% CI, -0.42 to 2.34]; P=.17), and reversible defect (-1.2 [95% CI, -12.50 to 10.12]; P=.84) were not statistically significant. There were no differences found in any of the secondary outcomes, including percent myocardial defect, total defect size, fixed defect size, regional wall motion, and clinical improvement. Conclusion Among patients with chronic ischemic heart failure, transendocardial injection of autologous BMCs compared with placebo did not improve LVESV, maximal oxygen consumption, or reversibility on SPECT. C1 [Lai, Dejian; Baraniuk, Sarah; Piller, Linda B.; Simpson, Lara M.; Bettencourt, Judy; Sayre, Shelly L.; Vojvodic, Rachel W.; Moye, Lemuel A.] Univ Texas Houston, Sch Publ Hlth, Houston, TX 77030 USA. [Loghin, Catalin] Univ Texas Houston, Sch Med, Houston, TX 77030 USA. [Penn, Marc S.] NE Ohio Med Univ, Akron, OH USA. [Anwaruddin, Saif] Univ Penn, Penn Heart & Vasc Hosp, Philadelphia, PA 19104 USA. [Gee, Adrian P.; Aguilar, David; Richman, Sara] Baylor Univ, Coll Med, Houston, TX 77030 USA. [Skarlatos, Sonia I.; Gordon, David J.; Ebert, Ray F.; Kwak, Minjung] NHLBI, Bethesda, MD 20892 USA. [Simari, Robert D.] Mayo Clin, Rochester, MN USA. [Perin, Emerson C.; Willerson, James T.; Silva, Guilherme V.; Smith, Deirdre; Westbrook, Lynette] St Lukes Episcopal Hosp, Texas Heart Inst, Houston, TX 77030 USA. [Pepine, Carl J.; Anderson, R. David; Cogle, Christopher R.; Handberg, Eileen] Univ Florida, Sch Med, Gainesville, FL USA. [Martin, A. Daniel] Univ Florida, Coll Publ Hlth & Hlth Profess, Gainesville, FL USA. [Henry, Timothy D.; Traverse, Jay H.; Olson, Rachel E.] Abbott NW Hosp, Minneapolis Heart Inst, Minneapolis, MN 55407 USA. [Henry, Timothy D.; Traverse, Jay H.; Taylor, Doris A.; Zierold, Claudia] Univ Minnesota, Sch Med, Minneapolis, MN 55455 USA. [Ellis, Stephen G.; Thomas, James D.; Geither, Carrie] Cleveland Clin Fdn, Cleveland, OH 44195 USA. [Zhao, David X. M.; Kronenberg, Marvin W.; Hatzopoulos, Antonis K.; Bowman, Sherry; Francescon, Judy] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. RP Moye, LA (reprint author), Univ Texas Houston, Sch Publ Hlth, 1200 Pressler,W-848, Houston, TX 77030 USA. EM lemmoye@msn.com RI Hatzopoulos, Antonis/D-2049-2010; Cogle, Christopher/H-1746-2016 OI Cogle, Christopher/0000-0001-5422-6863 FU National Heart, Lung, and Blood Institute (NHLBI); Amarin Pharma; Amorycyte; Angioblast/Mesoblast; Baxter Healthcare; Bayer Healthcare; Daiichi-Sankyo; GlaxoSmithKline; Lilly; Merck; National Institutes of Health; National Heart, Lung; Blood Institute; Pfizer; sanofi-aventis; Viron Therapeutics; Aastrom; Mesoblast; NHLBI [5 U01 HL087318-04, N01-HB-37164, HHSN268201000008C, N01-HB-37163, HHSN268201000007C]; National Center for Research Resources CTSA [UL1 TR000064] FX The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. All of the authors reported receiving research grant funding and support for travel expenses to meetings for the Cardiovascular Cell Therapy Research Network (CCTRN) from the National Heart, Lung, and Blood Institute (NHLBI). Dr Perin reported serving as a consultant to Amorcyte, Teva, Cytori, and Aldagen. Dr Pepine reported receiving research grants from Amarin Pharma, Amorycyte, Angioblast/Mesoblast, Baxter Healthcare, Bayer Healthcare, Daiichi-Sankyo, GlaxoSmithKline, Lilly, Merck, the National Institutes of Health, the National Heart, Lung, and Blood Institute, Pfizer, sanofi-aventis, Viron Therapeutics; serving as a consultant to Aastrom, Amarin Pharma, Amorycyte (scientific advisory board), Angioblast Systems (data and safety monitoring board), Lilly and County Community Foundation (data and safety monitoring board), and SLACK Inc; and holding 2 patents with the University of Florida. Dr Henry reported serving on steering committees for and receiving research grants from Aastrom and Mesoblast. Dr Martin reported receiving research grants from the University of Texas to provide an exercise testing core laboratory and received financial support for travel expenses. Dr Penn reported receiving research grants from Athersys Inc; serving as a board member for Juventas Therapeutics; serving as a consultant to Juventas Therapeutics and Aastrom Biosciences; and holding patents, receiving royalties, and owning stock in Juventas Therapeutics. Dr Hatzopoulos reported receiving payment for lectures from Washington University, University of Minnesota, and Tennessee State University. Dr Taylor reported receiving payment for a lecture at an annual meeting of the Cell Society. Dr Handberg reported receiving grants from Baxter, BDI, Amrin, Angioblast, Neostem, Harvest Technologies, Daiichi Sankyo, Pfizer, Gilead Sciences, and GlaxoSmithKline. Ms Olson reported receiving research grants from Mesoblast and Aastrom. Ms Geither reported receiving support for travel expenses to meetings from the Cleveland Clinic. Dr Aguilar reported being a consultant to Amylin Pharmaceutical in the area of diabetes and heart failure.; Funding for this trial was provided by the NHLBI under cooperative agreement 5 U01 HL087318-04. It also was supported in part by NHLBI contracts N01-HB-37164 and HHSN268201000008C awarded to the Molecular and Cellular Therapeutics Facility, University of Minnesota, and N01-HB-37163 and HHSN268201000007C awarded to the Cell Processing Facility, Baylor College of Medicine, and National Center for Research Resources CTSA grant UL1 TR000064 awarded to the University of Florida. The CCTRN also acknowledges its industry partners: Biosafe, Biologics Delivery Systems Group, and Cordis Corporation for their contributions of equipment and technical support during the conduct of the trial. NR 42 TC 198 Z9 207 U1 1 U2 14 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 25 PY 2012 VL 307 IS 16 BP 1717 EP 1726 DI 10.1001/jama.2012.418 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA 930OA UT WOS:000303147500025 PM 22447880 ER PT J AU Tao, NJ Nagahara, L Thundat, T Zhang, PM AF Tao, Nongjian Nagahara, Larry Thundat, Thomas Zhang, Peimeng TI Biography of Stuart Lindsay SO JOURNAL OF PHYSICS-CONDENSED MATTER LA English DT Biographical-Item C1 [Tao, Nongjian; Zhang, Peimeng] Arizona State Univ, Tempe, AZ 85287 USA. [Nagahara, Larry] NCI, Bethesda, MD 20892 USA. [Thundat, Thomas] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA. RP Tao, NJ (reprint author), Arizona State Univ, Tempe, AZ 85287 USA. EM nongjian.tao@asu.edu; nagaharl@mail.nih.gov; thundattg@ornl.gov; Peiming.Zhang@asu.edu NR 1 TC 0 Z9 0 U1 1 U2 9 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0953-8984 J9 J PHYS-CONDENS MAT JI J. Phys.-Condes. Matter PD APR 25 PY 2012 VL 24 IS 16 AR 160401 DI 10.1088/0953-8984/24/16/160401 PG 1 WC Physics, Condensed Matter SC Physics GA 922PE UT WOS:000302559200002 PM 22467144 ER PT J AU Dirisala, VR Jeevan, A Bix, G Yoshimura, T McMurray, DN AF Dirisala, Vijaya R. Jeevan, Amminikutty Bix, Gregory Yoshimura, Teizo McMurray, David N. TI Molecular cloning and expression of the IL-10 gene from guinea pigs SO GENE LA English DT Article DE IL-10 cDNA; Guinea pig; Recombinant protein ID CLASS-II EXPRESSION; MYCOBACTERIUM-TUBERCULOSIS; PULMONARY TUBERCULOSIS; TOTAL DEFICIENCY; ANIMAL-MODELS; INTERLEUKIN-10; CELLS; CYTOKINES; DISEASE; NEUTRALIZATION AB The Guinea pig (Cavia porcellus) is one of the most relevant small animals for modeling human tuberculosis (TB) in terms of susceptibility to low dose aerosol infection, the organization of granulomas, extrapulmonary dissemination and vaccine-induced protection. It is also considered to be a gold standard for a number of other infectious and non-infectious diseases: however, this animal model has a major disadvantage due to the lack of readily available immunological reagents. In the present study, we successfully cloned a cDNA for the critical Th2 cytokine, interleukin-10 (IL-10), from inbred Strain 2 guinea pigs using the DNA sequence information provided by the genome project. The complete open reading frame (ORF) consists of 537 base pairs which encodes a protein of 179 amino acids. This cDNA sequence exhibited 87% homology with human IL-10. Surprisingly, it showed only 84% homology with the previously published IL-10 sequence from the C4-deficient (C4D) guinea pig, leading us to clone IL-10 cDNA from the Hartley strain of guinea pig. The IL-10 gene from the Hartley strain showed 100% homology with the IL-10 sequence of Strain 2 guinea pigs. In order to validate the only published IL-10 sequence existing in Genbank reported from C4D guinea pigs, genomic DNA was isolated from tissues of C4D guinea pigs. Amplification with various sets of primers showed that the IL-10 sequence reported from C4D guinea pigs contained numerous errors. Hence the IL-10 sequence that is being reported by us replaces the earlier sequence making our IL-10 sequence to be the first one accurate from guinea pig. Recombinant guinea pig IL-10 proteins were subsequently expressed in both prokaryotic and eukaryotic cells, purified and were confirmed by N-terminal sequencing. Polyclonal anti-IL-10 antibodies were generated in rabbits using the recombinant IL-10 protein expressed in this study. Taken together, our results indicate that the DNA sequence information provided by the genome project is useful to directly clone much needed cDNAs necessary to study TB in the guinea pig. The newly cloned guinea pig IL-10 cDNA and recombinant proteins will serve as valuable resources for immunological studies in the guinea pig model of TB and other diseases. (C) 2012 Elsevier B.V. All rights reserved. C1 [Dirisala, Vijaya R.; Jeevan, Amminikutty; McMurray, David N.] Texas A&M Hlth Sci Ctr, Dept Microbial & Mol Pathogenesis, Coll Med, College Stn, TX 77843 USA. [Bix, Gregory] Texas A&M Hlth Sci Ctr, Dept Mol & Cellular Med, Coll Med, College Stn, TX 77843 USA. [Yoshimura, Teizo] NCI, Mol Immunoregulat Lab, Canc & Inflammat Program, Ctr Canc Res, Frederick, MD 21702 USA. RP Dirisala, VR (reprint author), Texas A&M Hlth Sci Ctr, Dept Microbial & Mol Pathogenesis, Coll Med, 407 Reynolds Med Bldg, College Stn, TX 77843 USA. EM dirisala@medicine.tamhsc.edu RI Dirisala, Vijaya/H-5038-2012; Dirisala, Vijaya/A-2120-2016 FU Colorado State University under NIH [HHSN 266200400091c] FX This work was supported by a subcontract from Colorado State University under NIH contract HHSN 266200400091c. The authors thank Dr. Larry Dangott of the Protein Chemistry Laboratory at Texas A & M University for successful protein sequencing and Ms. Cathryn Formichella for her good technical help. We are also very thankful to Dr. Patricia C. Giclas and her support team from National Jewish Health, Denver, Colorado for shipping tissues from C4D and C2D guinea pigs. NR 45 TC 6 Z9 7 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD APR 25 PY 2012 VL 498 IS 1 BP 120 EP 127 DI 10.1016/j.gene.2012.01.076 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 923AG UT WOS:000302589800019 PM 22349028 ER PT J AU Belshan, M Kimata, JT Brown, C Cheng, XG McCulley, A Larsen, A Thippeshappa, R Hodara, V Giavedoni, L Hirsch, V Ratner, L AF Belshan, Michael Kimata, Jason T. Brown, Charles Cheng, Xiaogang McCulley, Anna Larsen, Alison Thippeshappa, Rajesh Hodara, Vida Giavedoni, Luis Hirsch, Vanessa Ratner, Lee TI Vpx is Critical for SIVmne infection of pigtail macaques SO RETROVIROLOGY LA English DT Article DE Vpx; SIV; Macaques ID VIRUS TYPE-2 VPX; VIRAL PREINTEGRATION COMPLEX; NUCLEAR-LOCALIZATION SIGNAL; RESTRICTION FACTOR SAMHD1; CD4(+) T-CELLS; REVERSE TRANSCRIPTION; NONDIVIDING CELLS; IMMUNODEFICIENCY; REPLICATION; PROTEIN AB Background: Viral protein X (Vpx) of SIV has been reported to be important for establishing infection in vivo. Vpx has several different activities in vitro, promoting preintegration complex import into the nucleus in quiescent lymphocytes and overcoming a block in reverse transcription in macrophages. Vpx interacts with the DDB1-CUL4-DCAF1 E3 ligase complex, which may or may not be required for the ascribed functions. The goal of the current study was to determine whether these activities of Vpx are important in vivo. Results: An infectious, pathogenic clone of SIVmne was used to examine correlations between Vpx functions in vitro and in vivo. Three previously described HIV-2 Vpx mutants that were shown to be important for nuclear import of the preintegration complex in quiescent lymphocytes were constructed in SIVmne: A vpx-deleted virus, a truncation of Vpx at amino acid 102 that deletes the C-terminal proline-rich domain (X(102)), and a mutant with tyrosines 66, 69, and 71 changed to alanine (X(y-a)). All mutant viruses replicated similarly to wild type SIVmne027 in primary pigtail macaque PBMCs, and were only slightly retarded in CEMx174 cells. However, all the vpx mutant viruses were defective for replication in both human and pigtail monocyte-derived macrophages. PCR assays demonstrated that the efficiency of reverse transcription and the levels of viral integration in macrophages were substantially reduced for the vpx mutant viruses. In vitro, the X(y-a) mutant, but not the X(102) mutant lost interaction with DCAF1. The wild type SIVmne027 and the three vpx mutant SIVs were inoculated by the intrarectal route into pigtail macaques. Peak levels of plasma viremia of the vpx mutant SIVs were variable, but consistently lower than that observed in macaques infected with wild type SIVmne. In situ hybridization for SIV demonstrated that compared to wild type SIVmne infected macaques five of the six animals inoculated with the vpx mutant SIVs had only low levels of SIV-expressing cells in the rectum, most intestinal epithelial tissues, spleen, and mesenteric and peripheral nodes. Conclusions: This work demonstrates that the activities of Vpx to overcome restrictions in culture in vitro are also likely to be important for establishment of infection in vivo and suggest that both the nuclear localization and DCAF1-interaction functions of Vpx are critical in vivo. C1 [Cheng, Xiaogang; McCulley, Anna; Ratner, Lee] Washington Univ, Sch Med, Div Mol Oncol, St Louis, MO 63130 USA. [Belshan, Michael; Larsen, Alison] Creighton Univ, Dept Med Microbiol & Immunol, Omaha, NE 68178 USA. [Kimata, Jason T.; Thippeshappa, Rajesh] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. [Brown, Charles; Hirsch, Vanessa] NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA. [Hodara, Vida; Giavedoni, Luis] Texas Biomed Res Inst, SW Natl Primate Res Ctr, Dept Virol & Immunol, San Antonio, TX USA. [Ratner, Lee] Washington Univ, Div Oncol, St Louis, MO 63110 USA. RP Ratner, L (reprint author), Washington Univ, Sch Med, Div Mol Oncol, St Louis, MO 63130 USA. EM lratner@dom.wustl.edu FU NIAID; Creighton Health Futures Foundation; Nebraska Center for Virology [RR01635]; Southwest National Primate Research Center [RR13986]; PHS [AI047725, AI24745, AI55383] FX We thank Dr. Leland for care of animals, and Ms Campbell for assistance in processing samples. Funding for SIV RNA levels provided to Advanced Biosciences Labs through NIAID. This work was supported by Creighton Health Futures Foundation and Nebraska Center for Virology (RR01635) to MB, Southwest National Primate Research Center (RR13986), PHS grants AI047725 to JTK and AI24745 and AI55383 to LR. NR 42 TC 21 Z9 22 U1 0 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1742-4690 J9 RETROVIROLOGY JI Retrovirology PD APR 24 PY 2012 VL 9 AR 32 DI 10.1186/1742-4690-9-32 PG 13 WC Virology SC Virology GA 947ZY UT WOS:000304474400001 PM 22531456 ER PT J AU Lyko, B Hammershaimb, EA Nguitragool, W Wellems, TE Desai, SA AF Lyko, Brian Hammershaimb, Elizabeth A. Nguitragool, Wang Wellems, Thomas E. Desai, Sanjay A. TI A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates SO MALARIA JOURNAL LA English DT Article ID MICROTITER PLATES; PARASITE CLONES; CLONING AB Background: Molecular and cellular studies of Plasmodium falciparum require cloning of parasites by limiting dilution cultivation, typically performed in microplates. The parasite's slow replication rate combined with laborious methods for identification of positive wells has limited these studies. A new high-throughput method for detecting growth without compromising parasite viability is reported. Methods: In vitro parasite cultivation is associated with extracellular acidification. A survey of fluorescent pH indicators identified 5-(and-6)-carboxy SNARF-1 as a membrane-impermeant dye with a suitable pK(a) value. Conditions for facile detection of viable parasites in 96-well microplates were optimized and used for limiting dilution cloning of genetic cross progeny and transfected parasites. Results: 5-(and-6)-carboxy SNARF-1 is a two-emission wavelength dye that accurately reported extracellular pH in parasite cultures. It readily detected parasite growth in microplate wells and yielded results comparable to labour-intensive examination of Giemsa-stained smears. The dye is non-toxic, allowing parasite detection without transfer of culture material to additional plates for separate assays. This dye was used with high-throughput limiting dilution culture to generate additional progeny clones from the HB3 x Dd2 genetic cross. Conclusions: This fluorescence-based assay represents a low-cost, efficient method for detection of viable parasites in microplate wells; it can be easily expanded by automation. C1 [Lyko, Brian; Hammershaimb, Elizabeth A.; Nguitragool, Wang; Wellems, Thomas E.; Desai, Sanjay A.] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 USA. [Nguitragool, Wang] Mahidol Univ, Fac Trop Med, Bangkok, Thailand. RP Desai, SA (reprint author), NIAID, Lab Malaria & Vector Res, NIH, Room 3W-01,12735 Twinbrook Pkwy, Rockville, MD 20852 USA. EM sdesai@niaid.nih.gov FU National Institutes of Health, National Institute of Allergy and Infectious Diseases FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Diseases. NR 9 TC 11 Z9 11 U1 2 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2875 J9 MALARIA J JI Malar. J. PD APR 24 PY 2012 VL 11 AR 124 DI 10.1186/1475-2875-11-124 PG 5 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA 942LZ UT WOS:000304046800001 PM 22531353 ER PT J AU Matsuoka, T Yashiro, M Nishioka, N Hirakawa, K Olden, K Roberts, J AF Matsuoka, T. Yashiro, M. Nishioka, N. Hirakawa, K. Olden, K. Roberts, J. D. TI PI3K/Akt signalling is required for the attachment and spreading, and growth in vivo of metastatic scirrhous gastric carcinoma SO BRITISH JOURNAL OF CANCER LA English DT Article DE PI3 kinase; gastric carcinoma; adhesion; spreading; metastasis; integrin signalling ID FOCAL ADHESION KINASE; HUMAN CANCER; PHOSPHATIDYLINOSITOL 3-KINASE; PHOSPHOINOSITIDE 3-KINASE; STRUCTURAL BASIS; CELL-MIGRATION; SRC; PATHWAY; FAMILY; ACTIVATION AB BACKGROUND: PI3K/Akt (PKB) pathway has been shown in several cell types to be activated by ligands to cell surface integrins, leading to the metastasis of tumour cells. The signalling pathways involved in the metastatic spread of human scirrhous gastric carcinoma cells have not been defined. METHODS: The role of the PI3K/Akt pathway in an extensive peritoneal-seeding cell line, OCUM-2MD3 and a parental cell line, OCUM-2M, was investigated by assessing in vitro adhesion and spreading assay, and in vivo peritoneal metastatic model. We also examined the correlation of PI3K/Akt pathway with integrin signals by immunoprecipitations, using cells by transfection with mutant p85 (Delta p85). RESULTS: Adhesiveness and spreading of OCUM-2MD3 cells on collagen type IV was significantly decreased by PI3K inhibitors and expression of mutant p85, but not by inhibitors of protein kinase C (PKC) or extracellular signal-regulated kinase (ERK). Immunoprecipitation studies indicated that the PI3K/Akt pathway was associated with integrin signalling through Src and vinculin. In an in vivo experimental metastasis model, p85 inhibition reduced peritoneal metastasis of OCUM-2MD3 cells. CONCLUSION: PI3K/Akt signalling may be required for integrin-dependent attachment and spreading of scirrhous gastric carcinoma cells, and would be translated into generating better strategies to optimise their use in cancer clinical trials. British Journal of Cancer (2012) 106, 1535-1542. doi:10.1038/bjc.2012.107 www.bjcancer.com (C) 2012 Cancer Research UK C1 [Matsuoka, T.; Nishioka, N.; Olden, K.; Roberts, J. D.] NIEHS, US Dept HHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA. [Matsuoka, T.; Yashiro, M.; Nishioka, N.; Hirakawa, K.] Osaka City Univ, Dept Surg Oncol, Grad Sch Med, Osaka 558, Japan. RP Matsuoka, T (reprint author), NIEHS, US Dept HHS, Mol Carcinogenesis Lab, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. EM matsuoka@med.osaka-cu.ac.jp FU NIH; NIEHS FX We thank Dr Wataru Ogawa for providing wild-type and mutant p85 expression plasmids under the control of the SR promoter. We thank Dr Steven K. Akiyama for helpful discussions, and Dr Ron Cannon and Dr Richard DiAugustine of NIEHS for a careful review of the manuscript. This work was supported by the Intramural Research Program of the NIH and NIEHS. NR 43 TC 9 Z9 11 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0007-0920 J9 BRIT J CANCER JI Br. J. Cancer PD APR 24 PY 2012 VL 106 IS 9 BP 1535 EP 1542 DI 10.1038/bjc.2012.107 PG 8 WC Oncology SC Oncology GA 933SR UT WOS:000303383500012 PM 22531720 ER PT J AU Masison, DC AF Masison, Daniel C. TI Perfecting precision of predicting prion propensity SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Editorial Material ID BETA-SHEET STRUCTURE; FORM PRIONS; PROTEIN; AGGREGATION; SEQUENCE; DOMAINS; FIBRILS; REGIONS; YEAST C1 NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. RP Masison, DC (reprint author), NIDDK, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA. EM masisond@helix.nih.gov FU Intramural NIH HHS NR 20 TC 0 Z9 0 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 24 PY 2012 VL 109 IS 17 BP 6362 EP 6363 DI 10.1073/pnas.1203767109 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931WD UT WOS:000303249100014 PM 22499791 ER PT J AU Ma, WJ Halweg, CJ Menendez, D Resnick, MA AF Ma, Wenjian Halweg, Christopher J. Menendez, Daniel Resnick, Michael A. TI Differential effects of poly(ADP-ribose) polymerase inhibition on DNA break repair in human cells are revealed with Epstein-Barr virus SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE base excision repair; BRCA ID DOUBLE-STRAND BREAKS; BASE EXCISION-REPAIR; FIELD GEL-ELECTROPHORESIS; ADP-RIBOSE POLYMERASE; WILD-TYPE P53; SINGLE-STRAND; PARP INHIBITION; COMET ASSAY; SV40 DNA; RADIATION AB Poly(ADP-ribose) polymerase (PARP) inhibitors can generate synthetic lethality in cancer cells defective in homologous recombination. However, the mechanism(s) by which they affect DNA repair has not been established. Here we directly determined the effects of PARP inhibition and PARP1 depletion on the repair of ionizing radiation-induced single-and double-strand breaks (SSBs and DSBs) in human lymphoid cell lines. To do this, we developed an in vivo repair assay based on large endogenous Epstein-Barr virus (EBV) circular episomes. The EBV break assay provides the opportunity to assess quantitatively and simultaneously the induction and repair of SSBs and DSBs in human cells. Repair was efficient in G1 and G2 cells and was not dependent on functional p53. shRNA-mediated knockdown of PARP1 demonstrated that the PARP1 protein was not essential for SSB repair. Among 10 widely used PARP inhibitors, none affected DSB repair, although an inhibitor of DNA-dependent protein kinase was highly effective at reducing DSB repair. Only Olaparib and Iniparib, which are in clinical cancer therapy trials, as well as 4-AN inhibited SSB repair. However, a decrease in PARP1 expression reversed the ability of Iniparib to reduce SSB repair. Because Iniparib disrupts PARP1-DNA binding, the mechanism of inhibition does not appear to involve trapping PARP at SSBs. C1 [Ma, Wenjian; Halweg, Christopher J.; Menendez, Daniel; Resnick, Michael A.] NIEHS, Chromosome Stabil Sect, NIH, Res Triangle Pk, NC 27709 USA. RP Resnick, MA (reprint author), NIEHS, Chromosome Stabil Sect, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. EM resnick@niehs.nih.gov FU NIEHS (National Institutes of Health, Department of Health and Human Services) [1 Z01 ES065073] FX We thank Dr. Norman Sharpless, Dr. Julie Horton, Dr. Michelle Heacock, Dr. Kin Chan, and Jim Westmoreland for critical reading of the manuscript, and Dr. Charles Romeo and Dr. Negin Martin at the National Institute of Environmental Health Sciences (NIEHS) Viral Vector Core Laboratory for generating LCL35 shRNA cell lines. This work was supported by the Intramural Research Program of the NIEHS (National Institutes of Health, Department of Health and Human Services) under Project 1 Z01 ES065073 (to M.A.R.). NR 64 TC 17 Z9 17 U1 0 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 24 PY 2012 VL 109 IS 17 BP 6590 EP 6595 DI 10.1073/pnas.1118078109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931WD UT WOS:000303249100053 PM 22493268 ER PT J AU Arredondo, SA Cai, ML Takayama, Y MacDonald, NJ Anderson, DE Aravind, L Clore, GM Miller, LH AF Arredondo, Silvia A. Cai, Mengli Takayama, Yuki MacDonald, Nicholas J. Anderson, D. Eric Aravind, L. Clore, G. Marius Miller, Louis H. TI Structure of the Plasmodium 6-cysteine s48/45 domain SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE Plasmodium domain evolution; Escherichia coli periplasmic expression; solution conformation ID TRANSMISSION-BLOCKING IMMUNITY; GAMETE-SURFACE PROTEIN; TOXOPLASMA-GONDII; COMPARATIVE GENOMICS; BALANCING SELECTION; VACCINE CANDIDATE; ESCHERICHIA-COLI; SRS SUPERFAMILY; NMR STRUCTURE; HOST-CELLS AB The s48/45 domain was first noted in Plasmodium proteins more than 15 y ago. Previously believed to be unique to Plasmodium, the s48/45 domain is present in other aconoidasidans. In Plasmodium, members of the s48/45 family of proteins are localized on the surface of the parasite in different stages, mostly by glycosylphosphatydylinositol-anchoring. Members such as P52 and P36 seem to play a role in invasion of hepatocytes, and Pfs230 and Pfs48/45 are involved in fertilization in the sexual stages and have been consistently studied as targets of transmission-blocking vaccines for years. In this report, we present the molecular structure for the s48/45 domain corresponding to the C-terminal domain of the blood-stage protein Pf12 from Plasmodium falciparum, obtained by NMR. Our results indicate that this domain is a beta-sandwich formed by two sheets with a mixture of parallel and antiparallel strands. Of the six conserved cysteines, two pairs link the beta-sheets by two disulfide bonds, and the third pair forms a bond outside the core. The structure of the s48/45 domain conforms well to the previously defined surface antigen 1 (SAG1)-related-sequence (SRS) fold observed in the SAG family of surface antigens found in Toxoplasma gondii. Despite extreme sequence divergence, remarkable spatial conservation of one of the disulfide bonds is observed, supporting the hypothesis that the domains have evolved from a common ancestor. Furthermore, a homologous domain is present in ephrins, raising the possibility that the precursor of the s48/45 and SRS domains emerged from an ancient transfer to Apicomplexa from metazoan hosts. C1 [Aravind, L.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. [Arredondo, Silvia A.] NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. [MacDonald, Nicholas J.] NIAID, Lab Malaria Immunol & Vaccinol, NIH, Rockville, MD 20852 USA. [Miller, Louis H.] NIAID, Lab Malaria Vector Res, NIH, Rockville, MD 20852 USA. [Cai, Mengli; Takayama, Yuki; Clore, G. Marius] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. [Anderson, D. Eric] NIDDKD, Mass Spectrometry Facil, NIH, Bethesda, MD 20892 USA. RP Aravind, L (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. EM aravind@mail.nih.gov; mariusc@intra.niddk.nih.gov; lmiller@niaid.nih.gov RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 FU National Library of Medicine; National Institute of Allergy and Infectious Diseases; National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health FX We thank Susan Pierce (Laboratory of Immunogenetics) and Kim Williamson and Prakash Srinivasan (Laboratory of Malaria Vector Research) for very insightful discussions. This work was supported by the intramural funds from the National Library of Medicine (to L.A.), the National Institute of Allergy and Infectious Diseases (L.H.M.), and the National Institute of Diabetes and Digestive and Kidney Diseases (G.M.C.) of the National Institutes of Health. NR 65 TC 28 Z9 30 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 24 PY 2012 VL 109 IS 17 BP 6692 EP 6697 DI 10.1073/pnas.1204363109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931WD UT WOS:000303249100070 PM 22493233 ER PT J AU Buyske, S Wu, Y Carty, CL Cheng, IN Assimes, TL Dumitrescu, L Hindorff, LA Mitchell, S Ambite, JL Boerwinkle, E Buzkova, P Carlson, CS Cochran, B Duggan, D Eaton, CB Fesinmeyer, MD Franceschini, N Haessler, J Jenny, N Kang, HM Kooperberg, C Lin, Y Le Marchand, L Matise, TC Robinson, JG Rodriguez, C Schumacher, FR Voight, BF Young, A Manolio, TA Mohlke, KL Haiman, CA Peters, U Crawford, DC North, KE AF Buyske, Steven Wu, Ying Carty, Cara L. Cheng, Iona Assimes, Themistocles L. Dumitrescu, Logan Hindorff, Lucia A. Mitchell, Sabrina Ambite, Jose Luis Boerwinkle, Eric Buzkova, Petra Carlson, Chris S. Cochran, Barbara Duggan, David Eaton, Charles B. Fesinmeyer, Megan D. Franceschini, Nora Haessler, Jeffrey Jenny, Nancy Kang, Hyun Min Kooperberg, Charles Lin, Yi Le Marchand, Loic Matise, Tara C. Robinson, Jennifer G. Rodriguez, Carlos Schumacher, Fredrick R. Voight, Benjamin F. Young, Alicia Manolio, Teri A. Mohlke, Karen L. Haiman, Christopher A. Peters, Ulrike Crawford, Dana C. North, Kari E. TI Evaluation of the Metabochip Genotyping Array in African Americans and Implications for Fine Mapping of GWAS-Identified Loci: The PAGE Study SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; DENSITY-LIPOPROTEIN CHOLESTEROL; POPULATION; COMMON; SNPS; IMPUTATION; VARIANT; DESIGN; TOOL; MAP AB The Metabochip is a custom genotyping array designed for replication and fine mapping of metabolic, cardiovascular, and anthropometric trait loci and includes low frequency variation content identified from the 1000 Genomes Project. It has 196,725 SNPs concentrated in 257 genomic regions. We evaluated the Metabochip in 5,863 African Americans; 89% of all SNPs passed rigorous quality control with a call rate of 99.9%. Two examples illustrate the value of fine mapping with the Metabochip in African-ancestry populations. At CELSR2/PSRC1/SORT1, we found the strongest associated SNP for LDL-C to be rs12740374 (p = 3.5x10(-11)), a SNP indistinguishable from multiple SNPs in European ancestry samples due to high correlation. Its distinct signal supports functional studies elsewhere suggesting a causal role in LDL-C. At CETP we found rs17231520, with risk allele frequency 0.07 in African Americans, to be associated with HDL-C (p = 7.2x10(-36)). This variant is very rare in Europeans and not tagged in common GWAS arrays, but was identified as associated with HDL-C in African Americans in a single-gene study. Our results, one narrowing the risk interval and the other revealing an associated variant not found in Europeans, demonstrate the advantages of high-density genotyping of common and rare variation for fine mapping of trait loci in African American samples. C1 [Buyske, Steven] Rutgers State Univ, Dept Stat & Biostat, Piscataway, NJ 08855 USA. [Wu, Ying; Mohlke, Karen L.] Univ N Carolina, Dept Genet, Chapel Hill, NC USA. [Carty, Cara L.; Carlson, Chris S.; Fesinmeyer, Megan D.; Haessler, Jeffrey; Kooperberg, Charles; Lin, Yi; Young, Alicia; Peters, Ulrike] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Cheng, Iona; Le Marchand, Loic] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. [Assimes, Themistocles L.] Stanford Univ, Dept Med, Sch Med, Stanford, CA 94305 USA. [Dumitrescu, Logan; Crawford, Dana C.] Vanderbilt Univ, Ctr Human Genet Res, Nashville, TN USA. [Hindorff, Lucia A.; Manolio, Teri A.] NHGRI, Off Populat Genom, NIH, Bethesda, MD 20892 USA. [Mitchell, Sabrina; Crawford, Dana C.] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA. [Ambite, Jose Luis] Univ So Calif, Inst Informat Sci, Los Angeles, CA USA. [Boerwinkle, Eric] Univ Texas Hlth Sci Ctr, Ctr Human Genet, Houston, TX USA. [Buzkova, Petra] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Cochran, Barbara] Baylor Coll Med, Sponsored Programs, Houston, TX 77030 USA. [Duggan, David] Translat Genom Res Inst, Phoenix, AZ USA. [Eaton, Charles B.] Brown Univ, Ctr Primary Care & Prevent, Dept Family Med & Epidemiol, Alpert Med Sch, Providence, RI 02912 USA. [Franceschini, Nora; North, Kari E.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [Jenny, Nancy] Univ Vermont, Dept Pathol, Burlington, VT 05405 USA. [Kang, Hyun Min] Univ Michigan, Dept Biostat, Ann Arbor, MI 48109 USA. [Buyske, Steven; Matise, Tara C.] Rutgers State Univ, Dept Genet, Piscataway, NJ USA. [Robinson, Jennifer G.] Univ Iowa, Dept Epidemiol & Med, Iowa City, IA USA. [Rodriguez, Carlos] Wake Forest Univ, Bowman Gray Sch Med, Div Publ Hlth Sci, Winston Salem, NC USA. [Schumacher, Fredrick R.; Haiman, Christopher A.] Univ So Calif, Keck Sch Med, Los Angeles, CA 90033 USA. [Voight, Benjamin F.] Broad Inst Harvard & MIT, Cambridge, MA USA. [Mohlke, Karen L.; North, Kari E.] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA. RP Buyske, S (reprint author), Rutgers State Univ, Dept Stat & Biostat, Piscataway, NJ 08855 USA. EM kari_north@unc.edu RI Carty, Cara/B-8683-2013; Crawford, Dana/C-1054-2012; OI Buyske, Steven/0000-0001-8539-5416 FU National Human Genome Research Institute (NHGRI) [U01HG004803, U01HG004798, U01HG004802, U01HG004790, U01HG004801]; NHLBI [N01-HC-55015, N01-HC-55016, N01-HC-55018, N01-HC-55019, N01-HC-55020, N01-HC-55021, N01-HC-55022]; National Cancer Institute [R37CA54281, R01 CA63, P01CA33619, U01CA136792, U01CA98758]; National Heart, Lung, and Blood Institute (NHLBI) (NIH); U.S. Department of Health and Human Services [N01WH22110, 24152, 32100-2, 32105-6, 32108-9, 32111-13, 32115, 32118-32119, 32122, 42107-26, 42129-32, 44221]; National Institutes of Mental Health FX The Population Architecture Using Genomics and Epidemiology (PAGE) program is funded by the National Human Genome Research Institute (NHGRI), supported by U01HG004803 (Causal Variants Across the Life Course, known as CALiCo), U01HG004798 (Epidemiologic Architecture of Genes Linked to Environment, known as EAGLE), U01HG004802 (the Multiethnic Cohort, known as MEC), U01HG004790 (Women's Health Initiative, known as WHI), and U01HG004801 (Coordinating Center). The Atherosclerosis Risk in Communities (ARIC) Study is carried out as a collaborative study supported by NHLBI contracts N01-HC-55015, N01-HC-55016, N01-HC-55018, N01-HC-55019, N01-HC-55020, N01-HC-55021, N01-HC-55022. The Multiethnic Cohort (MEC) study is funded through the National Cancer Institute (R37CA54281, R01 CA63, P01CA33619, U01CA136792, and U01CA98758). The Women's Health Initiative (WHI) program is funded by the National Heart, Lung, and Blood Institute (NHLBI) (NIH) and by U.S. Department of Health and Human Services through contracts N01WH22110, 24152, 32100-2, 32105-6, 32108-9, 32111-13, 32115, 32118-32119, 32122, 42107-26, 42129-32, and 44221. The National Institutes of Mental Health also contributes to the support for the Coordinating Center. NHGRI collaborators (LAH and TAM) assisted in the study design, analysis, and preparation of the manuscript. NR 38 TC 29 Z9 30 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 23 PY 2012 VL 7 IS 4 AR e35651 DI 10.1371/journal.pone.0035651 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UP UT WOS:000305341000054 PM 22539988 ER PT J AU Pomerantz, O Paukner, A Terkel, J AF Pomerantz, Ori Paukner, Annika Terkel, Joseph TI Some stereotypic behaviors in rhesus macaques (Macaca mulatta) are correlated with both perseveration and the ability to cope with acute stressors SO BEHAVIOURAL BRAIN RESEARCH LA English DT Article DE Stereotypic behavior; Perseveration; Coping hypothesis; Basal ganglia; Psychological welfare ID ABNORMAL REPETITIVE BEHAVIORS; SELF-INJURIOUS-BEHAVIOR; BASAL GANGLIA; COPING RESPONSE; LABORATORY MICE; MONKEYS; SCHIZOPHRENIA; DISINHIBITION; PHYSIOLOGY; ANIMALS AB The most prevalent sub-group of abnormal repetitive behaviors among captive animals is that of stereotypies. Previous studies have demonstrated some resemblance between stereotypy in captive animals and in humans, including the involvement of neurological malfunctions that lead to the expression of stereotypies. This malfunction can be evaluated through the use of neuropsychological tasks that assess perseveration as implying a failure of the basal ganglia (BC) to operate properly. Other studies, in contrast, have suggested that stereotypies are the product of neurologically intact individuals reacting to the abnormal nature of their surroundings, and are possibly characterized by an adaptive feature that enables the subject to cope with such adversity. Employing neuropsychological tests and also measuring the levels of fecal corticoids in captive rhesus macaques, we tested the hypothesis that stereotypies are related both to brain pathology and to a coping mechanism with stress, resembling accounts by autistic individuals exhibiting basal ganglia malfunction, and who report a sense of relief when performing stereotypies. Self-directed and fine-motor stereotypies exhibited by the monkeys were positively correlated with perseveration, Suggesting BG malfunction; while self-directed stereotypies were also negatively correlated with an increase in fecal corticoids following a stress challenge, suggesting a related coping mechanism. We therefore suggest that not all repetitive, unvarying, and apparently functionless behaviors should be regarded as one homogeneous group of stereotypic behaviors; and that, reflecting reports from autistic individuals, self-directed stereotypies in captive rhesus monkeys are related both to brain pathology, and to an adaptive mechanism that allows those that express them to better cope with acute stressors. (C) 2012 Elsevier B.V. All rights reserved. C1 [Pomerantz, Ori; Terkel, Joseph] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel. [Paukner, Annika] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Comparat Ethol Lab, NIH, Anim Ctr, Poolesville, MD 20837 USA. RP Pomerantz, O (reprint author), Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel. EM oripomer@post.tau.ac.il RI Pomerantz, Ori/B-1869-2013 FU Intramural NIH HHS [Z99 HD999999] NR 51 TC 11 Z9 11 U1 1 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-4328 J9 BEHAV BRAIN RES JI Behav. Brain Res. PD APR 21 PY 2012 VL 230 IS 1 BP 274 EP 280 DI 10.1016/j.bbr.2012.02.019 PG 7 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA 973SJ UT WOS:000306379800033 PM 22366267 ER PT J AU Fitzgerald, PJ AF Fitzgerald, Paul J. TI The NMDA receptor may participate in widespread suppression of circuit level neural activity, in addition to a similarly prominent role in circuit level activation SO BEHAVIOURAL BRAIN RESEARCH LA English DT Review DE Glutamate; NMDA receptor; Epilepsy; Neurotoxicity; Knockout mouse; Electrophysiology; 2-Deoxyglucose; Functional brain imaging; Ketamine; Phencyclidine; MK-801 ID METHYL-D-ASPARTATE; BRAIN METABOLIC-ACTIVATION; MICE LACKING; PREFRONTAL CORTEX; PREPULSE INHIBITION; POTENTIAL RELEVANCE; DOPAMINE NEURONS; BLOOD-FLOW; RAT-BRAIN; 2-DEOXYGLUCOSE UPTAKE AB The N-methyl-D-aspartate receptor (NMDAR) plays a critical role in glutamatergically mediated neurotransmission, and thereby affects a wide range of brain circuits and important behavioral functions, not limited to learning and memory. While glutamate is classically considered to be the brain's principal excitatory neurotransmitter, there is also evidence the NMDAR plays a "functionally inhibitory" role, not in that it directly hyperpolarizes neurons but rather it suppresses circuit level neural activity, including through activation of GABAergic interneurons. This paper reviews data on the NMDAR "suppression" hypothesis (while also examining circuit level activation), with a focus on the following 6 lines of evidence: (1) epilepsy studies, (2) neurotoxicity studies, (3) mouse knockout studies of particular receptor subunits, (4) electrophysiological studies, (5) 2-deoxyglucose studies, and (6) functional brain imaging studies. For many of these lines of evidence, the review focuses on data from two well-characterized NMDAR antagonists, ketamine and phencyclidine. Also, evidence regarding the NMDAR and schizophrenia, including the psychotomimetic properties of ketamine and phencyclidine, cuts across several of the lines of evidence. The data suggest the NMDAR participates in activation, as well as widespread suppression, of circuit level neural activity, where the suppression may be particularly prominent in limbic circuits. (C) 2012 Elsevier B.V. All rights reserved. C1 [Fitzgerald, Paul J.] Johns Hopkins Univ, Zanvyl Krieger Mind Brain Inst, Solomon H Snyder Dept Neurosci, Baltimore, MD 21218 USA. RP Fitzgerald, PJ (reprint author), NIAAA, 5625 Fishers Lane,Room 2N09, Rockville, MD 20852 USA. EM pfitz@mbi.mb.jhu.edu NR 110 TC 13 Z9 14 U1 0 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-4328 J9 BEHAV BRAIN RES JI Behav. Brain Res. PD APR 21 PY 2012 VL 230 IS 1 BP 291 EP 298 DI 10.1016/j.bbr.2012.01.057 PG 8 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA 973SJ UT WOS:000306379800036 PM 22342923 ER PT J AU Chowell, G Viboud, C Simonsen, L Miller, MA Echevarria-Zuno, S Gonzalez-Leon, M Aburto, VHB AF Chowell, Gerardo Viboud, Cecile Simonsen, Lone Miller, Mark A. Echevarria-Zuno, Santiago Gonzalez-Leon, Margot Aburto, Victor H. Borja TI Impact of antiviral treatment and hospital admission delay on risk of death associated with 2009 A/H1N1 pandemic influenza in Mexico SO BMC INFECTIOUS DISEASES LA English DT Article DE 2009 A/H1N1 influenza pandemic; Neuraminidase inhibitors; Antivirals; Case fatality ratio; Multivariate logistic regression; Hospital admission delay; Pandemic wave; Mexico ID A H1N1 VIRUS; OSELTAMIVIR TREATMENT; ILL PATIENTS; INFECTION; CHILDREN; MORTALITY; SEVERITY; OUTCOMES; A(H1N1); CHILE AB Background: Increasing our understanding of the factors affecting the severity of the 2009 A/H1N1 influenza pandemic in different regions of the world could lead to improved clinical practice and mitigation strategies for future influenza pandemics. Even though a number of studies have shed light into the risk factors associated with severe outcomes of 2009 A/H1N1 influenza infections in different populations (e.g., [1-5]), analyses of the determinants of mortality risk spanning multiple pandemic waves and geographic regions are scarce. Between-country differences in the mortality burden of the 2009 pandemic could be linked to differences in influenza case management, underlying population health, or intrinsic differences in disease transmission [6]. Additional studies elucidating the determinants of disease severity globally are warranted to guide prevention efforts in future influenza pandemics. In Mexico, the 2009 A/H1N1 influenza pandemic was characterized by a three-wave pattern occurring in the spring, summer, and fall of 2009 with substantial geographical heterogeneity [7]. A recent study suggests that Mexico experienced high excess mortality burden during the 2009 A/H1N1 influenza pandemic relative to other countries [6]. However, an assessment of potential factors that contributed to the relatively high pandemic death toll in Mexico are lacking. Here, we fill this gap by analyzing a large series of laboratory-confirmed A/H1N1 influenza cases, hospitalizations, and deaths monitored by the Mexican Social Security medical system during April 1 through December 31, 2009 in Mexico. In particular, we quantify the association between disease severity, hospital admission delays, and neuraminidase inhibitor use by demographic characteristics, pandemic wave, and geographic regions of Mexico. Methods: We analyzed a large series of laboratory-confirmed pandemic A/H1N1 influenza cases from a prospective surveillance system maintained by the Mexican Social Security system, April-December 2009. We considered a spectrum of disease severity encompassing outpatient visits, hospitalizations, and deaths, and recorded demographic and geographic information on individual patients. We assessed the impact of neuraminidase inhibitor treatment and hospital admission delay (<= > 2 days after disease onset) on the risk of death by multivariate logistic regression. Results: Approximately 50% of all A/H1N1-positive patients received antiviral medication during the Spring and Summer 2009 pandemic waves in Mexico while only 9% of A/H1N1 cases received antiviral medications during the fall wave (P < 0.0001). After adjustment for age, gender, and geography, antiviral treatment significantly reduced the risk of death (OR = 0.52 (95% CI: 0.30, 0.90)) while longer hospital admission delays increased the risk of death by 2.8-fold (95% CI: 2.25, 3.41). Conclusions: Our findings underscore the potential impact of decreasing admission delays and increasing antiviral use to mitigate the mortality burden of future influenza pandemics. C1 [Chowell, Gerardo] Arizona State Univ, Sch Human Evolut & Social Change, Math Computat & Modeling Sci Ctr, Tempe, AZ 85069 USA. [Simonsen, Lone] George Washington Univ, Sch Publ Hlth, Dept Global Hlth, Washington, DC USA. [Simonsen, Lone] George Washington Univ, Hlth Serv, Washington, DC USA. [Echevarria-Zuno, Santiago] Inst Mexicano Seguro Social, Direcc Prestaciones Med, Mexico City 03100, DF, Mexico. [Chowell, Gerardo] Arizona State Univ, Div Epidemiol & Populat Studies, Fogarty Int Ctr, NIH,Sch Human Evolut & Social Change, Tempe, AZ USA. RP Chowell, G (reprint author), Arizona State Univ, Sch Human Evolut & Social Change, Math Computat & Modeling Sci Ctr, Tempe, AZ 85069 USA. EM gchowell@asu.edu RI Chowell, Gerardo/F-5038-2012; OI Chowell, Gerardo/0000-0003-2194-2251; Simonsen, Lone/0000-0003-1535-8526 FU International Influenza Unit, Office of Global Health Affairs, Department of Health and Human Services; RAPIDD program of the Science and Technology Directorate; Department of Homeland Security; Fogarty International Center FX This research was conducted in the context of the MISMS Study, an ongoing international collaborative effort to understand influenza epidemiological and evolutionary patterns, led by the Fogarty International Center, National Institutes of Health (http://www.origem.info/misms/index.php). The MISMS study is funded by the International Influenza Unit, Office of Global Health Affairs, Department of Health and Human Services. LS acknowledges support from the RAPIDD program of the Science and Technology Directorate, Department of Homeland Security, and the Fogarty International Center. NR 51 TC 6 Z9 6 U1 0 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2334 J9 BMC INFECT DIS JI BMC Infect. Dis. PD APR 20 PY 2012 VL 12 AR 97 DI 10.1186/1471-2334-12-97 PG 11 WC Infectious Diseases SC Infectious Diseases GA 009DZ UT WOS:000309007100001 PM 22520743 ER PT J AU Minguzzi, S Molloy, AM Peadar, K Mills, J Scott, JM Troendle, J Pangilinan, F Brody, L Parle-McDermott, A AF Minguzzi, Stefano Molloy, Anne M. Peadar, Kirke Mills, James Scott, John M. Troendle, James Pangilinan, Faith Brody, Lawrence Parle-McDermott, Anne TI Genotyping of a tri-allelic polymorphism by a novel melting curve assay in MTHFD1L: an association study of nonsyndromic Cleft in Ireland SO BMC MEDICAL GENETICS LA English DT Article ID MITOCHONDRIAL C-1-TETRAHYDROFOLATE SYNTHASE; NEURAL-TUBE DEFECTS; OROFACIAL CLEFTS; RISK-FACTOR; FOLIC-ACID; GENE POLYMORPHISMS; MESSENGER-RNA; FOLATE INTAKE; LIP; PALATE AB Background: Polymorphisms within the MTHFD1L gene were previously associated with risk of neural tube defects in Ireland. We sought to test the most significant MTHFD1L polymorphisms for an association with risk of cleft in an Irish cohort. This required the development of a new melting curve assay to genotype the technically challenging MTHFD1L triallelic deletion/insertion polymorphism (rs3832406). Methods: Melting curve analysis was used to genotype the MTHFD1L triallelic deletion/insertion polymorphism (rs3832406) and a Single Nucleotide Polymorphism rs17080476 in an Irish cohort consisting of 981 Irish case-parent trios and 1,008 controls. Tests for association with nonsyndromic cleft lip with or without cleft palate and cleft palate included case/control analysis, mother/control analysis and Transmission Disequilibrium Tests of case-parent trios. Results: A successful melting curve genotyping assay was developed for the deletion/insertion polymorphism (rs3832406). The TDT analysis initially showed that the rs3832406 polymorphism was associated with isolated cleft lip with or without cleft palate. However, corrected p-values indicated that this association was not significant. Conclusions: Melting Curve Analysis can be employed to successfully genotype challenging polymorphisms such as the MTHFD1L triallelic deletion/insertion polymorphism (DIP) reported here (rs3832406) and is a viable alternative to capillary electrophoresis. Corrected p-values indicate no association between MTHFD1L and risk of cleft in an Irish cohort. C1 [Minguzzi, Stefano; Parle-McDermott, Anne] Dublin City Univ, Sch Biotechnol, Nutr Genom Grp, Dublin 9, Ireland. [Molloy, Anne M.] Univ Dublin Trinity Coll, Sch Med, Dublin 2, Ireland. [Peadar, Kirke] Hlth Res Board, Child Hlth Epidemiol Unit, Dublin, Ireland. [Mills, James; Troendle, James] Eunice Kennedy Shriver Natl Inst Hlth, Dept Hlth & Human Serv, Bethesda, MD USA. [Scott, John M.] Univ Dublin Trinity Coll, Sch Immunol & Biochem, Dublin 2, Ireland. [Pangilinan, Faith; Brody, Lawrence] NHGRI, Mol Pathogenesis Sect, Genome Technol Branch, Bethesda, MD 20892 USA. RP Parle-McDermott, A (reprint author), Dublin City Univ, Sch Biotechnol, Nutr Genom Grp, Dublin 9, Ireland. EM anne.parle-mcdermott@dcu.ie OI Molloy, Anne/0000-0002-1688-9049 FU IRCSET Embark initiative; Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development; Health Research Board of Ireland FX This work was supported by the IRCSET Embark initiative. The authors sincerely thank all the patients and families who participated in the study, the Cleft Lip and Palate Association of Ireland, and the Dublin Cleft Centre team. Recruitment of the Irish cleft cohort was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the Health Research Board of Ireland. NR 30 TC 2 Z9 2 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2350 J9 BMC MED GENET JI BMC Med. Genet. PD APR 20 PY 2012 VL 13 AR 29 DI 10.1186/1471-2350-13-29 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 994VE UT WOS:000307961000001 PM 22520921 ER PT J AU Jeang, KT AF Jeang, Kuan-Teh TI Epidermal growth factor receptor (EGFR) biology and Wnt developmental signaling highlight research endeavors of SCBA award winners SO CELL AND BIOSCIENCE LA English DT Article AB This month's Cell and Bioscience highlights review articles by Mien-Chie Hung on EGFR biology and Yingzi Yang on Wnt signaling. Dr. Hung was the 2011 Society of Chinese Bioscientists in America (SCBA) Presidential Award winner. Dr. Yang was the 2011 SCBA Outstanding Young Investigator Award winner. C1 NIAID, Bethesda, MD 20892 USA. RP Jeang, KT (reprint author), NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM kjeang@niaid.nih.gov FU National Institute of Allergy and Infectious Diseases FX I thank Yun-Bo Shi for asking me to write this brief commentary. Work in my laboratory is supported in part by intramural funding from the National Institute of Allergy and Infectious Diseases. This commentary is my personal opinion and does not necessarily represent the views of my employer, the National Institutes of Health, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2045-3701 J9 CELL BIOSCI JI Cell Biosci. PD APR 20 PY 2012 VL 2 AR 12 DI 10.1186/2045-3701-2-12 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 988SV UT WOS:000307511900001 PM 22520574 ER PT J AU Cowan, RE Fregly, BJ Boninger, ML Chan, L Rodgers, MM Reinkensmeyer, DJ AF Cowan, Rachel E. Fregly, Benjamin J. Boninger, Michael L. Chan, Leighton Rodgers, Mary M. Reinkensmeyer, David J. TI Recent trends in assistive technology for mobility SO JOURNAL OF NEUROENGINEERING AND REHABILITATION LA English DT Review DE Disability; Assistive technology; Robotics ID ANKLE-FOOT ORTHOSIS; SYSTEM; PROSTHETICS; WHEELCHAIR; VISION; DESIGN; INJURY; GAIT AB Loss of physical mobility makes maximal participation in desired activities more difficult and in the worst case fully prevents participation. This paper surveys recent work in assistive technology to improve mobility for persons with a disability, drawing on examples observed during a tour of academic and industrial research sites in Europe. The underlying theme of this recent work is a more seamless integration of the capabilities of the user and the assistive technology. This improved integration spans diverse technologies, including powered wheelchairs, prosthetic limbs, functional electrical stimulation, and wearable exoskeletons. Improved integration is being accomplished in three ways: 1) improving the assistive technology mechanics; 2) improving the user-technology physical interface; and 3) sharing of control between the user and the technology. We provide an overview of these improvements in user-technology integration and discuss whether such improvements have the potential to be transformative for people with mobility impairments. C1 [Cowan, Rachel E.] Univ Miami, Miller Sch Med, Dept Neurol Surg, Miami Project Cure Paralysis, Miami, FL 33136 USA. [Fregly, Benjamin J.] Univ Florida, Dept Mech & Aerosp Engn, Gainesville, FL 32611 USA. [Boninger, Michael L.] Univ Pittsburgh, Dept Phys Med & Rehabil, Sch Med, Pittsburgh, PA 15260 USA. [Chan, Leighton] NIH, Dept Rehabil Med, Ctr Clin, Bethesda, MD 20892 USA. [Rodgers, Mary M.] Univ Maryland, Dept Phys Therapy & Rehabil Sci, Baltimore, MD 21201 USA. [Rodgers, Mary M.] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA. [Reinkensmeyer, David J.] Univ Calif Irvine, Dept Mech & Aerosp Engn, Dept Anat & Neurobiol, Dept Biomed Engn, Irvine, CA 92697 USA. [Boninger, Michael L.] VA Pittsburgh Healthcare Syst, Human Engn Res Labs, Pittsburgh, PA 15206 USA. RP Cowan, RE (reprint author), Univ Miami, Miller Sch Med, Dept Neurol Surg, Miami Project Cure Paralysis, 1095 NW 14th Terrace, Miami, FL 33136 USA. EM rcowan@med.miami.edu RI Fregly, Benjamin/O-6860-2016; OI Fregly, Benjamin/0000-0003-1166-4358; Boninger, Michael/0000-0001-6966-919X NR 31 TC 25 Z9 25 U1 6 U2 57 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-0003 J9 J NEUROENG REHABIL JI J. NeuroEng. Rehabil. PD APR 20 PY 2012 VL 9 AR 20 DI 10.1186/1743-0003-9-20 PG 8 WC Engineering, Biomedical; Neurosciences; Rehabilitation SC Engineering; Neurosciences & Neurology; Rehabilitation GA 981ZY UT WOS:000307012700001 PM 22520500 ER PT J AU Yang, YZ AF Yang, Yingzi TI Wnt signaling in development and disease SO CELL AND BIOSCIENCE LA English DT Review ID PLANAR CELL POLARITY; BRACHYDACTYLY TYPE-B; LEFT-RIGHT ASYMMETRY; NEURAL-TUBE DEFECTS; RECEPTOR-RELATED PROTEIN-5; RECESSIVE ROBINOW-SYNDROME; SYNOVIAL JOINT FORMATION; BONE-MASS; SCLEROSTIN ANTIBODY; NODAL EXPRESSION AB Cell signaling mediated by morphogens is essential to coordinate growth and patterning, two key processes that govern the formation of a complex multi-cellular organism. During growth and patterning, cells are specified by both quantitative and directional information. While quantitative information regulates cell proliferation and differentiation, directional information is conveyed in the form of cell polarities instructed by local and global cues. Major morphogens like Wnts play critical roles in embryonic development and they are also important in maintaining tissue homeostasis. Abnormal regulation of these signaling events leads to a diverse array of devastating diseases including cancer. Wnts transduce their signals through several distinct pathways and they regulate vertebrate embryonic development by providing both quantitative and directional information. Here, taking the developing skeletal system as an example, we review our work on Wnt signaling pathways in various aspects of development. We focus particularly on our most recent findings that showed that in vertebrates, Wnt5a acts as a global cue to establishing planar cell polarity (PCP). Our work suggests that Wnt morphogens regulate development by integrating quantitative and directional information. Our work also provides important insights in disease like Robinow syndrome, brachydactyly type B1 (BDB1) and spina bifida, which can be caused by human mutations in the Wnt/PCP signaling pathway. C1 NHGRI, Genet Dis Res Branch, Bethesda, MD 20892 USA. RP Yang, YZ (reprint author), NHGRI, Genet Dis Res Branch, 49 Convent DriveMSC 4472, Bethesda, MD 20892 USA. EM yingzi@mail.nih.gov FU Division of Intramural Research of the National Institutes of Health FX This review is based on Dr Yingzi Yang's Young Investigator Award Lecture at the SCBA biennial meeting in Guangzhou, China, in 2011. Dr Yang would like to thank all current and previous members of the Yang lab for the hard work that led to this award. This work was supported by the Division of Intramural Research of the National Institutes of Health. NR 66 TC 32 Z9 36 U1 0 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2045-3701 J9 CELL BIOSCI JI Cell Biosci. PD APR 20 PY 2012 VL 2 AR 14 DI 10.1186/2045-3701-2-14 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 982RY UT WOS:000307064300001 PM 22520685 ER PT J AU Fujisawa, T Rubin, B Suzuki, A Patel, PS Gahl, WA Joshi, BH Puri, RK AF Fujisawa, Toshio Rubin, Benjamin Suzuki, Akiko Patel, Prabhudas S. Gahl, William A. Joshi, Bharat H. Puri, Raj K. TI Cysteamine Suppresses Invasion, Metastasis and Prolongs Survival by Inhibiting Matrix Metalloproteinases in a Mouse Model of Human Pancreatic Cancer SO PLOS ONE LA English DT Article ID INTERLEUKIN-13 RECEPTOR ALPHA-2; NEPHROPATHIC CYSTINOSIS; CELL-LINES; IN-VITRO; CHILDREN; CARCINOMA; GROWTH; RATS; ANGIOGENESIS; PROGRESSION AB Background: Cysteamine, an anti-oxidant aminothiol, is the treatment of choice for nephropathic cystinosis, a rare lysosomal storage disease. Cysteamine is a chemo-sensitization and radioprotection agent and its antitumor effects have been investigated in various tumor cell lines and chemical induced carcinogenesis. Here, we investigated whether cysteamine has anti-tumor and anti-metastatic effects in transplantable human pancreatic cancer, an aggressive metastatic disease. Methodology/Principal Findings: Cysteamine's anti-invasion effects were studied by matrigel invasion and cell migration assays in 10 pancreatic cancer cell lines. To study mechanism of action, we examined cell viability and matrix metalloproteinases (MMPs) activity in the cysteamine-treated cells. We also examined cysteamine's anti-metastasis effect in two orthotopic murine models of human pancreatic cancer by measuring peritoneal metastasis and survival of animals. Cysteamine inhibited both migration and invasion of all ten pancreatic cancer cell lines at concentrations (<25 mM) that caused no toxicity to cells. It significantly decreased MMPs activity (IC50 38-460 mu M) and zymographic gelatinase activity in a dose dependent manner in vitro and in vivo; while mRNA and protein levels of MMP-9, MMP-12 and MMP-14 were slightly increased using the highest cysteamine concentration. In vivo, cysteamine significantly decreased metastasis in two established pancreatic tumor models, although it did not affect the size of primary tumors. Additionally, cysteamine prolonged survival of mice in a dose-dependent manner without causing any toxicity. Similar to the in vitro results, MMP activity was significantly decreased in animal tumors treated with cysteamine. Cysteamine had no clinical or preclinical adverse effects in the host even at the highest dose. Conclusions/Significance: Our results suggest that cysteamine, an agent with a proven safety profile, may be useful for inhibition of metastasis and prolonging the survival of a host with pancreatic cancer. C1 [Fujisawa, Toshio; Suzuki, Akiko; Patel, Prabhudas S.; Joshi, Bharat H.; Puri, Raj K.] Ctr Biol Evaluat & Res Food & Drug Adm, Tumor Vaccines & Biotechnol Branch, Div Cellular & Gene Therapies, Bethesda, MD USA. [Rubin, Benjamin] Johns Hopkins Sch Med, Suburban Hosp, Dept Ophthalmol, Bethesda, MD USA. [Gahl, William A.] NHGRI, Sect Human Biochem Genet, Med Genet Branch, NIH, Bethesda, MD 20892 USA. RP Puri, RK (reprint author), Ctr Biol Evaluat & Res Food & Drug Adm, Tumor Vaccines & Biotechnol Branch, Div Cellular & Gene Therapies, Bethesda, MD USA. EM raj.puri@fda.hhs.gov NR 39 TC 12 Z9 13 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e34437 DI 10.1371/journal.pone.0034437 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200014 PM 22532830 ER PT J AU Graham, JC Zarbl, H AF Graham, Jessica C. Zarbl, Helmut TI Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells SO PLOS ONE LA English DT Article ID IN-VITRO SELECTION; NUCLEIC-ACID APTAMERS; TUMOR-SUPPRESSOR; NONTUMORIGENIC REVERTANTS; EXPONENTIAL ENRICHMENT; SYSTEMATIC EVOLUTION; MACULAR DEGENERATION; HUMAN-PAPILLOMAVIRUS; E7 ONCOPROTEIN; LIGANDS AB Background: Disease-specific biomarkers are an important tool for the timely and effective management of pathological conditions, including determination of susceptibility, diagnosis, and monitoring efficacy of preventive or therapeutic strategies. Aptamers, comprising single-stranded or double-stranded DNA or RNA, can serve as biomarkers of disease or biological states. Aptamers can bind to specific epitopes on macromolecules by virtue of their three dimensional structures and, much like antibodies, aptamers can be used to target specific epitopes on the basis of their molecular shape. The Systematic Evolution of Ligands by EXponential enrichment (SELEX) is the approach used to select high affinity aptamers for specific macromolecular targets from among the >10(13) oligomers comprising typical random oligomer libraries. In the present study, we used live cell-based SELEX to identify DNA aptamers which recognize cell surface differences between HPV-transformed cervical carcinoma cancer cells and isogenic, nontumorigenic, revertant cell lines. Methodology/Principal Findings: Whole-cell SELEX methodology was adapted for use with adherent cell lines (which we termed Adherent Cell-SELEX (AC-SELEX)). Using this approach, we identified high affinity aptamers (nanomolar range K-d) to epitopes specific to the cell surface of two nontumorigenic, nontumorigenic revertants derived from the human cervical cancer HeLa cell line, and demonstrated the loss of these epitopes in another human papillomavirus transformed cervical cancer cell line (SiHa). We also performed preliminary investigation of the aptamer epitopes and their binding characteristics. Conclusions/Significance: Using AC-SELEX we have generated several aptamers that have high affinity and specificity to the nontumorigenic, revertant of HPV-transformed cervical cancer cells. These aptamers can be used to identify new biomarkers that are related to carcinogenesis. Panels of aptamers, such as these may be useful in predicting the tumorigenic potential and properties of cancer biopsies and aid in the effective management of pathological conditions (diagnosis, predicted outcome, and treatment options). C1 [Graham, Jessica C.; Zarbl, Helmut] Univ Med & Dent New Jersey, Sch Med, Dept Environm & Occupat Med, Piscataway, NJ 08854 USA. [Zarbl, Helmut] Canc Inst New Jersey, Div Publ Hlth Sci, Program Carcinogenesis & Chemoprevent, New Brunswick, NJ USA. [Zarbl, Helmut] Univ Med & Dent New Jersey, NIEHS Ctr Environm Exposures & Dis, Environm & Occupat Hlth Sci Inst, Piscataway, NJ 08854 USA. RP Zarbl, H (reprint author), Univ Med & Dent New Jersey, Sch Med, Dept Environm & Occupat Med, Piscataway, NJ 08854 USA. EM zarbl@eohsi.rutgers.edu FU Public Health Services Grant [NIEHS P30ES005022]; New Jersey Commission on Cancer Research [09-1962-CCR-EO] FX Supported by Public Health Services Grant #NIEHS P30ES005022 (HZ), a fellowship to JCG from the New Jersey Commission on Cancer Research, Award #: 09-1962-CCR-EO, and institutional support from the Robert Wood Johnson Medical School, UMDNJ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 25 Z9 31 U1 2 U2 51 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e36103 DI 10.1371/journal.pone.0036103 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200128 PM 22536456 ER PT J AU Merrins, MJ Bertram, R Sherman, A Satin, LS AF Merrins, Matthew J. Bertram, Richard Sherman, Arthur Satin, Leslie S. TI Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase Modulates Oscillations of Pancreatic Islet Metabolism SO PLOS ONE LA English DT Article ID LIVER 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE; SITE-DIRECTED MUTAGENESIS; PULSATILE INSULIN-SECRETION; FRUCTOSE 2,6-BISPHOSPHATE; GLUCOSE-METABOLISM; CRYSTAL-STRUCTURE; GLYCOLYTIC OSCILLATIONS; BETA-CELLS; MUSCLE PHOSPHOFRUCTOKINASE; LIVING CELLS AB Pulses of insulin from pancreatic beta-cells help maintain blood glucose in a narrow range, although the source of these pulses is unclear. It has been proposed that a positive feedback circuit exists within the glycolytic pathway, the autocatalytic activation of phosphofructokinase-1 (PFK1), which endows pancreatic beta-cells with the ability to generate oscillations in metabolism. Flux through PFK1 is controlled by the bifunctional enzyme PFK2/FBPase2 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) in two ways: via (1) production/degradation of fructose-2,6-bisphosphate (Fru2,6-BP), a potent allosteric activator of PFK1, as well as (2) direct activation of glucokinase due to a protein-protein interaction. In this study, we used a combination of live-cell imaging and mathematical modeling to examine the effects of inducibly-expressed PFK2/FBPase2 mutants on glucose-induced Ca2+ pulsatility in mouse islets. Irrespective of the ability to bind glucokinase, mutants of PFK2/FBPase2 that increased the kinase: phosphatase ratio reduced the period and amplitude of Ca2+ oscillations. Mutants which reduced the kinase: phosphatase ratio had the opposite effect. These results indicate that the main effect of the bifunctional enzyme on islet pulsatility is due to Fru2,6-BP alteration of the threshold for autocatalytic activation of PFK1 by Fru1,6-BP. Using computational models based on PFK1-generated islet oscillations, we then illustrated how moderate elevation of Fru-2,6-BP can increase the frequency of glycolytic oscillations while reducing their amplitude, with sufficiently high activation resulting in termination of slow oscillations. The concordance we observed between PFK2/FBPase2-induced modulation of islet oscillations and the models of PFK1-driven oscillations furthermore suggests that metabolic oscillations, like those found in yeast and skeletal muscle, are shaped early in glycolysis. C1 [Merrins, Matthew J.; Satin, Leslie S.] Univ Michigan, Dept Pharmacol, Ann Arbor, MI 48109 USA. [Merrins, Matthew J.; Satin, Leslie S.] Univ Michigan, Brehm Diabet Ctr, Ann Arbor, MI 48109 USA. [Bertram, Richard] Florida State Univ, Dept Math, Tallahassee, FL 32306 USA. [Bertram, Richard] Florida State Univ, Program Neurosci, Tallahassee, FL 32306 USA. [Bertram, Richard] Florida State Univ, Program Mol Biophys, Tallahassee, FL 32306 USA. [Sherman, Arthur] NIDDKD, Lab Biol Modeling, NIH, Bethesda, MD 20892 USA. RP Merrins, MJ (reprint author), Univ Michigan, Dept Pharmacol, Ann Arbor, MI 48109 USA. EM mmerrins@umich.edu OI Merrins, Matthew J./0000-0003-1599-9227 FU National Institutes of Health [F32DK085960, R01DK46409]; National Science Foundation [NSF-DMS0917664]; NIH/NIDDK; Michigan Diabetes Research and Training Center (MDRTC) Morphology and Image Analysis Core [DK20572] FX This work was supported by the National Institutes of Health (F32DK085960 to MJM, R01DK46409 to LS), the National Science Foundation (NSF-DMS0917664 to RB), and the NIH/NIDDK Intramural Research Program (AS). Additional support was provided by the Michigan Diabetes Research and Training Center (MDRTC) Morphology and Image Analysis Core (DK20572). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 12 Z9 12 U1 0 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e34036 DI 10.1371/journal.pone.0034036 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200009 PM 22532827 ER PT J AU Moore, JM Rabaia, NA Smith, LE Fagerlie, S Gurley, K Loukinov, D Disteche, CM Collins, SJ Kemp, CJ Lobanenkov, VV Filippova, GN AF Moore, James M. Rabaia, Natalia A. Smith, Leslie E. Fagerlie, Sara Gurley, Kay Loukinov, Dmitry Disteche, Christine M. Collins, Steven J. Kemp, Christopher J. Lobanenkov, Victor V. Filippova, Galina N. TI Loss of Maternal CTCF Is Associated with Peri-Implantation Lethality of Ctcf Null Embryos SO PLOS ONE LA English DT Article ID ENHANCER-BLOCKING ACTIVITY; C-MYC GENE; PROTEIN CTCF; ZYGOTIC TRANSITION; PREIMPLANTATION EMBRYO; GENOME ACTIVATION; X-INACTIVATION; BINDING-SITES; MOUSE; EXPRESSION AB CTCF is a highly conserved, multifunctional zinc finger protein involved in critical aspects of gene regulation including transcription regulation, chromatin insulation, genomic imprinting, X-chromosome inactivation, and higher order chromatin organization. Such multifunctional properties of CTCF suggest an essential role in development. Indeed, a previous report on maternal depletion of CTCF suggested that CTCF is essential for pre-implantation development. To distinguish between the effects of maternal and zygotic expression of CTCF, we studied pre-implantation development in mice harboring a complete loss of function Ctcf knockout allele. Although we demonstrated that homozygous deletion of Ctcf is early embryonically lethal, in contrast to previous observations, we showed that the Ctcf nullizygous embryos developed up to the blastocyst stage (E3.5) followed by peri-implantation lethality (E4.5-E5.5). Moreover, one-cell stage Ctcf nullizygous embryos cultured ex vivo developed to the 16-32 cell stage with no obvious abnormalities. Using a single embryo assay that allowed both genotype and mRNA expression analyses of the same embryo, we demonstrated that pre-implantation development of the Ctcf nullizygous embryos was associated with the retention of the maternal wild type Ctcf mRNA. Loss of this stable maternal transcript was temporally associated with loss of CTCF protein expression, apoptosis of the developing embryo, and failure to further develop an inner cell mass and trophoectoderm ex vivo. This indicates that CTCF expression is critical to early embryogenesis and loss of its expression rapidly leads to apoptosis at a very early developmental stage. This is the first study documenting the presence of the stable maternal Ctcf transcript in the blastocyst stage embryos. Furthermore, in the presence of maternal CTCF, zygotic CTCF expression does not seem to be required for pre-implantation development. C1 [Moore, James M.; Rabaia, Natalia A.; Smith, Leslie E.; Fagerlie, Sara; Gurley, Kay; Collins, Steven J.; Kemp, Christopher J.; Filippova, Galina N.] Fred Hutchinson Canc Res Ctr, Human Biol Div, Seattle, WA 98104 USA. [Loukinov, Dmitry; Lobanenkov, Victor V.] NIAID, Mol Pathol Sect, Immunopathol Lab, NIH, Rockville, MD USA. [Disteche, Christine M.] Univ Washington, Dept Pathol, Seattle, WA 98195 USA. RP Filippova, GN (reprint author), Fred Hutchinson Canc Res Ctr, Human Biol Div, 1124 Columbia St, Seattle, WA 98104 USA. EM gfilippo@fhcrc.org OI Lobanenkov, Victor/0000-0001-6665-3635 FU National Institutes of Health [CA68360]; [GM 46883] FX This work was supported by National Institutes of Health grant CA68360 (G.N.F. and V. V. L.) and in part by grant GM 46883 (C. M. D.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 17 Z9 18 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e34915 DI 10.1371/journal.pone.0034915 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200027 PM 22532833 ER PT J AU Patel, S Park, H Bonato, P Chan, L Rodgers, M AF Patel, Shyamal Park, Hyung Bonato, Paolo Chan, Leighton Rodgers, Mary TI A review of wearable sensors and systems with application in rehabilitation SO JOURNAL OF NEUROENGINEERING AND REHABILITATION LA English DT Review DE Wearable sensors and systems; Home monitoring; Telemedicine; Smart home ID OBSTRUCTIVE PULMONARY-DISEASE; HEALTH-CARE REFORM; PARKINSONS-DISEASE; PHYSICAL-ACTIVITY; MONITORING-SYSTEM; ACTIVITY PATTERNS; SUBACUTE STROKE; FALL DETECTION; ACCELEROMETRY; TECHNOLOGY AB The aim of this review paper is to summarize recent developments in the field of wearable sensors and systems that are relevant to the field of rehabilitation. The growing body of work focused on the application of wearable technology to monitor older adults and subjects with chronic conditions in the home and community settings justifies the emphasis of this review paper on summarizing clinical applications of wearable technology currently undergoing assessment rather than describing the development of new wearable sensors and systems. A short description of key enabling technologies (i.e. sensor technology, communication technology, and data analysis techniques) that have allowed researchers to implement wearable systems is followed by a detailed description of major areas of application of wearable technology. Applications described in this review paper include those that focus on health and wellness, safety, home rehabilitation, assessment of treatment efficacy, and early detection of disorders. The integration of wearable and ambient sensors is discussed in the context of achieving home monitoring of older adults and subjects with chronic conditions. Future work required to advance the field toward clinical deployment of wearable sensors and systems is discussed. C1 [Rodgers, Mary] Univ Maryland, Sch Med, Dept Phys Therapy & Rehabil Sci, Baltimore, MD 21201 USA. [Patel, Shyamal; Bonato, Paolo] Harvard Univ, Sch Med, Spaulding Rehabil Hosp, Dept Phys Med & Rehabil, Boston, MA USA. [Patel, Shyamal] Northeastern Univ, Dept Elect & Comp Engn, Boston, MA 02115 USA. [Park, Hyung; Chan, Leighton] NIH, Dept Rehabil Med, Ctr Clin, Bethesda, MD 20892 USA. [Bonato, Paolo] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA. [Rodgers, Mary] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD USA. RP Rodgers, M (reprint author), Univ Maryland, Sch Med, Dept Phys Therapy & Rehabil Sci, Baltimore, MD 21201 USA. EM MRodgers@som.umaryland.edu RI Park, Hyung-Soon/B-3334-2010 OI Park, Hyung-Soon/0000-0003-4274-7420 FU Intramural NIH HHS [Z99 CL999999] NR 108 TC 239 Z9 245 U1 34 U2 171 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-0003 J9 J NEUROENG REHABIL JI J. NeuroEng. Rehabil. PD APR 20 PY 2012 VL 9 AR 21 DI 10.1186/1743-0003-9-21 PG 17 WC Engineering, Biomedical; Neurosciences; Rehabilitation SC Engineering; Neurosciences & Neurology; Rehabilitation GA 951YO UT WOS:000304756900001 PM 22520559 ER PT J AU Philpott, CC AF Philpott, Caroline C. TI Coming into View: Eukaryotic Iron Chaperones and Intracellular Iron Delivery SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Review ID HYPOXIA-INDUCIBLE FACTOR; SACCHAROMYCES-CEREVISIAE; SULFUR CLUSTER; MONOTHIOL GLUTAREDOXIN; PROTEIN; HIF; FRATAXIN; HYDROXYLASE; LOCALIZATION; FAMILY AB Eukaryotic cells contain hundreds of metalloproteins, and ensuring that each protein receives the correct metal ion is a critical task for cells. Recent work in budding yeast and mammalian cells has uncovered a system of iron delivery operating in the cytosolic compartment that involves monothiol glutaredoxins, which bind iron in the form of iron-sulfur clusters, and poly(rC)-binding proteins, which bind Fe(II) directly. In yeast cells, cytosolic monothiol glutaredoxins are required for the formation of heme and iron-sulfur clusters and the metallation of some non-heme iron enzymes. Poly(rC)-binding proteins can act as iron chaperones, delivering iron to target non-heme enzymes through direct protein-protein interactions. Although the molecular details have yet to be explored, these proteins, acting independently or together, may represent the basic cellular machinery for intracellular iron delivery. C1 NIDDK, Liver Dis Branch, NIH, Bethesda, MD 20892 USA. RP Philpott, CC (reprint author), NIDDK, Liver Dis Branch, NIH, Bethesda, MD 20892 USA. EM carolinep@intra.niddk.nih.gov FU National Institutes of Health of NIDDK FX This work was supported, in whole or in part, by the National Institutes of Health Intramural Research Program of NIDDK. This is the second article in the Thematic Minireview Series on Metals in Biology 2012. NR 47 TC 44 Z9 47 U1 1 U2 17 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 20 PY 2012 VL 287 IS 17 BP 13518 EP 13523 DI 10.1074/jbc.R111.326876 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941VZ UT WOS:000303996300003 PM 22389494 ER PT J AU Ji, YW Sun, SY Xu, AM Bhargava, P Yang, L Lam, KSL Gao, B Lee, CH Kersten, S Qi, L AF Ji, Yewei Sun, Shengyi Xu, Aimin Bhargava, Prerna Yang, Liu Lam, Karen S. L. Gao, Bin Lee, Chih-Hao Kersten, Sander Qi, Ling TI Activation of Natural Killer T Cells Promotes M2 Macrophage Polarization in Adipose Tissue and Improves Systemic Glucose Tolerance via Interleukin-4 (IL-4)/STAT6 Protein Signaling Axis in Obesity SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID INVARIANT NKT CELLS; INSULIN-RESISTANCE; ALTERNATIVE ACTIVATION; MICROBIAL INFECTION; MICE; INFLAMMATION; RECEPTOR; PATHWAY; DISEASE; FAT AB Natural killer T (NKT) cells are important therapeutic targets in various disease models and are under clinical trials for cancer patients. However, their function in obesity and type 2 diabetes remains unclear. Our data show that adipose tissues of both mice and humans contain a population of type 1 NKT cells, whose abundance decreases with increased adiposity and insulin resistance. Although loss-of-function of NKT cells had no effect on glucose tolerance in animals with prolonged high fat diet feeding, activation of NKT cells by lipid agonist alpha-galactosylceramide enhances alternative macrophage polarization in adipose tissue and improves glucose homeostasis in animals at different stages of obesity. Furthermore, the effect of NKT cells is largely mediated by the IL-4/STAT6 signaling axis in obese adipose tissue. Thus, our data identify a novel therapeutic target for the treatment of obesity-associated inflammation and type 2 diabetes. C1 [Ji, Yewei; Kersten, Sander; Qi, Ling] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Sun, Shengyi; Yang, Liu; Qi, Ling] Cornell Univ, Grad Program Biochem Mol & Cell Biol, Ithaca, NY 14853 USA. [Xu, Aimin; Lam, Karen S. L.] Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China. [Xu, Aimin; Lam, Karen S. L.] Univ Hong Kong, Ctr Heart Brain Hormone & Hlth Aging, Hong Kong, Hong Kong, Peoples R China. [Bhargava, Prerna; Lee, Chih-Hao] Harvard Univ, Sch Publ Hlth, Dept Genet, Boston, MA 02115 USA. [Bhargava, Prerna; Lee, Chih-Hao] Harvard Univ, Sch Publ Hlth, Dept Complex Dis & Nutr, Boston, MA 02115 USA. [Gao, Bin] NIAAA, Lab Liver Dis, Rockville, MD 20852 USA. [Kersten, Sander] Wageningen Univ, Div Human Nutr, Nutr Metab & Genom Grp, NL-6700 EV Wageningen, Netherlands. RP Qi, L (reprint author), Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. EM lq35@cornell.edu RI Kersten, Sander/A-1116-2011; Xu, Aimin/D-3291-2013; Ji, Yewei/F-5040-2014; OI Kersten, Sander/0000-0003-4488-7734; Xu, Aimin/0000-0002-0668-033X; Ji, Yewei/0000-0002-4249-6812; Sun, Shengyi/0000-0002-1734-3902 FU National Institutes of Health from NIDDK [R01DK075046]; NIDDK [R01DK082582, R01DK082582-S1]; Hong Kong Collaborative Research Fund Grant [HKU4/CRF/10]; Netherlands Nutrigenomics Centre; Cornell startup package; American Diabetes Association [7-08-JF-47, 1-12-CD-04]; Howard Hughes Medical Institute FX This work was supported, in whole or in part, by National Institutes of Health Grant R01DK075046 from NIDDK (to C. H. L.) and Grants R01DK082582 and R01DK082582-S1 from NIDDK (to L. Q.). This work was also supported by Hong Kong Collaborative Research Fund Grant HKU4/CRF/10 (to A. X. and K. S. L. L.), Netherlands Nutrigenomics Centre (to S. K.), Cornell startup package, and American Diabetes Association Grants 7-08-JF-47 and 1-12-CD-04.; Supported by the International Student Research Fellowship from Howard Hughes Medical Institute. NR 57 TC 77 Z9 78 U1 2 U2 17 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 20 PY 2012 VL 287 IS 17 BP 13561 EP 13571 DI 10.1074/jbc.M112.350066 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941VZ UT WOS:000303996300013 PM 22396530 ER PT J AU Timofeeva, OA Chasovskikh, S Lonskaya, I Tarasova, NI Khavrutskii, L Tarasov, SG Zhang, XP Korostyshevskiy, VR Cheema, A Zhang, LH Dakshanamurthy, S Brown, ML Dritschilo, A AF Timofeeva, Olga A. Chasovskikh, Sergey Lonskaya, Irina Tarasova, Nadya I. Khavrutskii, Lyuba Tarasov, Sergey G. Zhang, Xueping Korostyshevskiy, Valeriy R. Cheema, Amrita Zhang, Lihua Dakshanamurthy, Sivanesan Brown, Milton L. Dritschilo, Anatoly TI Mechanisms of Unphosphorylated STAT3 Transcription actor Binding to DNA SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ATOMIC-FORCE MICROSCOPY; SCAFFOLD/MATRIX-ATTACHED REGIONS; CRUCIFORM STRUCTURES; PROTEINS; SITES; GENE; DIFFERENTIATION; THERMOPHORESIS; EXPRESSION; IDENTIFICATION AB Phosphorylation of signal transducer and activator of transcription 3 (STAT3) on a single tyrosine residue in response to growth factors, cytokines, interferons, and oncogenes activates its dimerization, translocation to the nucleus, binding to the interferon gamma (gamma)-activated sequence (GAS) DNA-binding site and activation of transcription of target genes. STAT3 is constitutively phosphorylated in various cancers and drives gene expression from GAS-containing promoters to promote tumorigenesis. Recently, roles for unphosphorylated STAT3 (U-STAT3) have been described in response to cytokine stimulation, in cancers, and in maintenance of heterochromatin stability. However, the mechanisms underlying U-STAT3 binding to DNA has not been fully investigated. Here, we explore STAT3-DNA interactions by atomic force microscopy (AFM) imaging. We observed that U-STAT3 molecules bind to the GAS DNA-binding site as dimers and monomers. In addition, we observed that U-STAT3 binds to AT-rich DNA sequence sites and recognizes specific DNA structures, such as 4-way junctions and DNA nodes, within negatively supercoiled plasmid DNA. These structures are important for chromatin organization and our data suggest a role for U-STAT3 as a chromatin/genome organizer. Unexpectedly, we found that a C-terminal truncated 67.5-kDa STAT3 isoform recognizes single-stranded spacers within cruciform structures that also have a role in chromatin organization and gene expression. This isoform appears to be abundant in the nuclei of cancer cells and, therefore, may have a role in regulation of gene expression. Taken together, our data highlight novel mechanisms by which U-STAT3 binds to DNA and supports U-STAT3 function as a transcriptional activator and a chromatin/genomic organizer. C1 [Timofeeva, Olga A.; Zhang, Xueping; Cheema, Amrita; Zhang, Lihua; Dakshanamurthy, Sivanesan; Brown, Milton L.; Dritschilo, Anatoly] Georgetown Univ, Med Ctr, Dept Oncol, Washington, DC 20057 USA. [Timofeeva, Olga A.; Chasovskikh, Sergey; Zhang, Xueping; Dritschilo, Anatoly] Georgetown Univ, Med Ctr, Dept Radiat Med, Washington, DC 20057 USA. [Lonskaya, Irina; Brown, Milton L.] Georgetown Univ, Med Ctr, Dept Neurosci, Washington, DC 20057 USA. [Korostyshevskiy, Valeriy R.] Georgetown Univ, Med Ctr, Dept Biostat Bioinformat & Biomath, Washington, DC 20057 USA. [Brown, Milton L.] Georgetown Univ, Med Ctr, Lombardi Comprehens Canc Ctr, Drug Discovery Program, Washington, DC 20057 USA. [Tarasova, Nadya I.; Khavrutskii, Lyuba] NCI Frederick, Canc & Inflammat Program, Frederick, MD 21702 USA. [Tarasova, Nadya I.] NCI Frederick, Struct Biophys Lab, Biophys Resource Core, Frederick, MD 21702 USA. RP Timofeeva, OA (reprint author), Georgetown Univ, Med Ctr, Dept Oncol, Res Bldg,R E216,3970 Reservoir Rd NW, Washington, DC 20057 USA. EM oat@georgetown.edu FU National Institutes of Health [CA56036-08]; American Cancer Society [IRG 97-152-17]; National Institutes of Health of the NCI Center for Cancer Research FX This work was supported, in whole or in part, by the National Institutes of Health Grant CA56036-08, American Cancer Society Grant IRG 97-152-17 (to O. T.) and by the National Institutes of Health Intramural Research Program of the NCI Center for Cancer Research. NR 53 TC 35 Z9 36 U1 2 U2 17 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 20 PY 2012 VL 287 IS 17 BP 14192 EP 14200 DI 10.1074/jbc.M111.323899 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 941VZ UT WOS:000303996300071 PM 22378781 ER PT J AU Reinkensmeyer, DJ Bonato, P Boninger, ML Chan, L Cowan, RE Fregly, BJ Rodgers, MM AF Reinkensmeyer, David J. Bonato, Paolo Boninger, Michael L. Chan, Leighton Cowan, Rachel E. Fregly, Benjamin J. Rodgers, Mary M. TI Major trends in mobility technology research and development: Overview of the results of the NSF-WTEC European study SO JOURNAL OF NEUROENGINEERING AND REHABILITATION LA English DT Editorial Material AB Mobility technologies, including wheelchairs, prostheses, joint replacements, assistive devices, and therapeutic exercise equipment help millions of people participate in desired life activities. Yet, these technologies are not yet fully transformative because many desired activities cannot be pursued or are difficult to pursue for the millions of individuals with mobility related impairments. This WTEC study, initiated and funded by the National Science Foundation, was designed to gather information on European innovations and trends in technology that might lead to greater mobility for a wider range of people. What might these transformative technologies be and how might they arise? Based on visits to leading mobility technology research labs in western Europe, the WTEC panel identified eight major trends in mobility technology research. This commentary summarizes these trends, which are then described in detail in companion papers appearing in this special issue. C1 [Reinkensmeyer, David J.] Univ Calif Irvine, Dept Biomed Engn, Dept Anat & Neurobiol, Dept Mech & Aerosp Engn, Irvine, CA 92697 USA. [Bonato, Paolo] Harvard Univ, Sch Med, Spaulding Rehabil Hosp, Dept PM&R, Boston, MA 02114 USA. [Boninger, Michael L.] Univ Pittsburgh, Sch Med, Dept Phys Med & Rehabil, Pittsburgh, PA 15260 USA. [Boninger, Michael L.] VA Pittsburgh Healthcare Syst, Pittsburgh, PA 15206 USA. [Chan, Leighton] NIH, Dept Rehabil Med, Ctr Clin, Bethesda, MD 20892 USA. [Cowan, Rachel E.] Univ Miami, Miller Sch Med, Dept Neurol Surg, Miami Project Cure Paralysis, Miami, FL 33136 USA. [Fregly, Benjamin J.] Univ Florida, Dept Mech & Aerosp Engn, Gainesville, FL 32611 USA. [Rodgers, Mary M.] Univ Maryland, Dept Phys Therapy & Rehabil Sci, Baltimore, MD 21201 USA. [Rodgers, Mary M.] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA. RP Reinkensmeyer, DJ (reprint author), Univ Calif Irvine, Dept Biomed Engn, Dept Anat & Neurobiol, Dept Mech & Aerosp Engn, 4200 Engn Gateway, Irvine, CA 92697 USA. EM dreinken@uci.edu RI Fregly, Benjamin/O-6860-2016; OI Fregly, Benjamin/0000-0003-1166-4358; Boninger, Michael/0000-0001-6966-919X NR 0 TC 10 Z9 10 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-0003 J9 J NEUROENG REHABIL JI J. NeuroEng. Rehabil. PD APR 20 PY 2012 VL 9 AR 22 DI 10.1186/1743-0003-9-22 PG 4 WC Engineering, Biomedical; Neurosciences; Rehabilitation SC Engineering; Neurosciences & Neurology; Rehabilitation GA 940AO UT WOS:000303862600001 PM 22520596 ER PT J AU Jiwani, S Ohr, RJ Fischer, ER Hackstadt, T Alvarado, S Romero, A Jewett, TJ AF Jiwani, Shahanawaz Ohr, Ryan J. Fischer, Elizabeth R. Hackstadt, Ted Alvarado, Stephenie Romero, Adriana Jewett, Travis J. TI Chlamydia trachomatis Tarp cooperates with the Arp2/3 complex to increase the rate of actin polymerization SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article DE Chlamydia trachomatis; Tarp; Arp2/3 complex; Actin ID TYROSINE KINASES; N-WASP; PROTEIN; RECRUITMENT; CELLS; ENTRY; PHOSPHORYLATION; MECHANISMS; NUCLEATION; FILAMENTS AB Actin polymerization is required for Chlamydia trachomatis entry into nonphagocytic host cells. Host and chlamydial actin nucleators are essential for internalization of chlamydiae by eukaryotic cells. The host cell Arp2/3 complex and the chlamydial translocated actin recruiting phosphoprotein (Tarp) are both required for entry. Tarp and the Arp2/3 complex exhibit unique actin polymerization kinetics individually, but the molecular details of how these two actin nucleators cooperate to promote bacterial entry is not understood. In this study we provide biochemical evidence that the two actin nucleators act synergistically by co-opting the unique attributes of each to enhance the dynamics of actin filament formation. This process is independent of Tarp phosphorylation. We further demonstrate that Tarp colocalization with actin filaments is independent of the Tarp phosphorylation domain. The results are consistent with a model in which chlamydial and host cell actin nucleators cooperate to increase the rate of actin filament formation. (C) 2012 Elsevier Inc. All rights reserved. C1 [Jiwani, Shahanawaz; Ohr, Ryan J.; Alvarado, Stephenie; Romero, Adriana; Jewett, Travis J.] Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Orlando, FL 32827 USA. [Fischer, Elizabeth R.; Hackstadt, Ted] NIAID, Host Parasite Interact Sect, Intracellular Parasites Lab, Rocky Mt Labs,NIH, Hamilton, MT 59840 USA. RP Jewett, TJ (reprint author), Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, 6900 Lake Nona Blvd, Orlando, FL 32827 USA. EM travis.jewett@ucf.edu FU NIAID, NIH K [5K22AI81729-2]; University of Central Florida; NIAID, NIH FX The authors wish to recognize members of Mollie W. Jewett laboratory for helpful discussions as well as acknowledge the technical assistance of Talia Chavez and Brenda Nguyen. This work was supported by the NIAID, NIH K award 5K22AI81729-2 and the University of Central Florida to T.J.J. and the intramural research program of the NIAID, NIH to T.H. NR 31 TC 11 Z9 11 U1 1 U2 3 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD APR 20 PY 2012 VL 420 IS 4 BP 816 EP 821 DI 10.1016/j.bbrc.2012.03.080 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 936VW UT WOS:000303619100021 PM 22465117 ER PT J AU Alfano, CM Imayama, I Neuhouser, ML Kiecolt-Glaser, JK Smith, AW Meeske, K McTiernan, A Bernstein, L Baumgartner, KB Ulrich, CM Ballard-Barbash, R AF Alfano, Catherine M. Imayama, Ikuyo Neuhouser, Marian L. Kiecolt-Glaser, Janice K. Smith, Ashley Wilder Meeske, Kathleen McTiernan, Anne Bernstein, Leslie Baumgartner, Kathy B. Ulrich, Cornelia M. Ballard-Barbash, Rachel TI Fatigue, Inflammation, and omega-3 and omega-6 Fatty Acid Intake Among Breast Cancer Survivors SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID C-REACTIVE PROTEIN; CORONARY-ARTERY-DISEASE; TUMOR-NECROSIS-FACTOR; FISH-OIL; OMEGA-3-FATTY-ACID SUPPLEMENTATION; DIETARY SUPPLEMENTATION; SEX-HORMONES; HOT FLASHES; BIOMARKERS; WOMEN AB Purpose Evidence suggests that inflammation may drive fatigue in cancer survivors. Research in healthy populations has shown reduced inflammation with higher dietary intake of omega-3 polyunsaturated fatty acids (PUFAs), which could potentially reduce fatigue. This study investigated fatigue, inflammation, and intake of omega-3 and omega-6 PUFAs among breast cancer survivors. Methods Six hundred thirty-three survivors (mean age, 56 years; stage I to IIIA) participating in the Health, Eating, Activity, and Lifestyle Study completed a food frequency/dietary supplement questionnaire and provided a blood sample assayed for C-reactive protein (CRP) and serum amyloid A (30 months after diagnosis) and completed the Piper Fatigue Scale and Short Form-36 (SF-36) vitality scale (39 months after diagnosis). Analysis of covariance and logistic regression models tested relationships between inflammation and fatigue, inflammation and omega-3 and omega-6 PUFA intake, and PUFA intake and fatigue, controlling for three incremental levels of confounders. Fatigue was analyzed continuously (Piper scales) and dichotomously (SF-36 vitality <= 50). Results Behavioral (P = .003) and sensory (P = .001) fatigue scale scores were higher by increasing CRP tertile; relationships were attenuated after adjustment for medication use and comorbidity. Survivors with high CRP had 1.8 times greater odds of fatigue after full adjustment (P < .05). Higher intake of omega-6 relative to omega-3 PUFAs was associated with greater CRP (P = .01 after full adjustment) and greater odds of fatigue (odds ratio, 2.6 for the highest v lowest intake; P < .05). Conclusion Results link higher intake of omega-3 PUFAs, decreased inflammation, and decreased physical aspects of fatigue. Future studies should test whether omega-3 supplementation may reduce fatigue among significantly fatigued breast cancer survivors. C1 [Alfano, Catherine M.] NCI, Off Canc Survivorship, NIH, Bethesda, MD 20892 USA. [Imayama, Ikuyo; Neuhouser, Marian L.; McTiernan, Anne; Ulrich, Cornelia M.] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [McTiernan, Anne] Univ Washington, Seattle, WA 98195 USA. [Kiecolt-Glaser, Janice K.] Ohio State Univ, Coll Med, Columbus, OH 43210 USA. [Meeske, Kathleen] Univ So Calif, Los Angeles, CA USA. [Bernstein, Leslie] City Hope Natl Med Ctr, Duarte, CA 91010 USA. [Baumgartner, Kathy B.] Univ Louisville, Louisville, KY 40292 USA. [Ulrich, Cornelia M.] German Canc Res Ctr, D-6900 Heidelberg, Germany. RP Alfano, CM (reprint author), NCI, Off Canc Survivorship, NIH, 6116 Execut Blvd,Ste 404, Bethesda, MD 20892 USA. EM alfanoc@mail.nih.gov RI Kiecolt-Glaser, Janice/A-3236-2009 OI Kiecolt-Glaser, Janice/0000-0003-4900-9578 FU National Cancer Institute (NCI) [N01-CN-75036-20, NO1-CN-05228, NO1-PC-67010, CA131029]; National Institutes of Health [M01-RR-00037]; Wyeth; [NCRR M01-RR-0997] FX Supported by National Cancer Institute (NCI) Contracts No. N01-CN-75036-20, NO1-CN-05228, and NO1-PC-67010. Portions of this work were conducted through the Clinical Research Center at the University of Washington and supported by the National Institutes of Health Grant No. M01-RR-00037 or through the University of New Mexico supported by Grant No. NCRR M01-RR-0997. J.K.K.-G. was supported by NCI Grant No. CA131029.; Research Funding: Janice K. Kiecolt-Glaser, National Institutes of Health; Anne McTiernan, Wyeth NR 60 TC 37 Z9 41 U1 1 U2 11 PU AMER SOC CLINICAL ONCOLOGY PI ALEXANDRIA PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD APR 20 PY 2012 VL 30 IS 12 BP 1280 EP 1287 DI 10.1200/JCO.2011.36.4109 PG 8 WC Oncology SC Oncology GA 938TR UT WOS:000303756500011 PM 22412148 ER PT J AU Casanova, JL Holland, SM Notarangelo, LD AF Casanova, Jean-Laurent Holland, Steven M. Notarangelo, Luigi D. TI Inborn Errors of Human JAKs and STATs SO IMMUNITY LA English DT Review ID HYPER-IGE SYNDROME; SEVERE COMBINED IMMUNODEFICIENCY; INTERLEUKIN-2-RECEPTOR GAMMA-CHAIN; CHRONIC MUCOCUTANEOUS CANDIDIASIS; COMBINED IMMUNE-DEFICIENCY; REGULATORY T-CELLS; DEFECTIVE LYMPHOID DEVELOPMENT; FOLLICULAR-HELPER-CELLS; IL-2 RECEPTOR; CUTTING EDGE AB Inborn errors of the genes encoding two of the four human JAKs (JAK3 and TYK2) and three of the six human STATs (STAT1, STA T3, and STAT5B) have been described. We review the disorders arising from mutations in these five genes, highlighting the way in which the molecular and cellular pathogenesis of these conditions has been clarified by the discovery of inborn errors of cytokines, hormones, and their receptors, including those interacting with JAKs and STATs. The phenotypic similarities between mice and humans lacking individual JAK-STAT components suggest that the functions of JAKs and STATs are largely conserved in mammals. However, a wide array of phenotypic differences has emerged between mice and humans carrying biallelic null alleles of JAK3, TYK2, STAT1, or STAT5B. Moreover, the high degree of allelic heterogeneity at the human JAK3, TYK2, STAT1, and STAT3 loci has revealed highly diverse immunological and clinical phenotypes, which had not been anticipated. C1 [Casanova, Jean-Laurent] Rockefeller Univ, Rockefeller Univ Hosp, St Giles Lab Human Genet Infect Dis, Rockefeller Branch, New York, NY 10065 USA. [Casanova, Jean-Laurent] Univ Paris 05, Necker Branch, Lab Human Genet Infect Dis, Paris 75005, France. [Casanova, Jean-Laurent] Inserm, Necker Med Sch, Paris 75005, France. [Holland, Steven M.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Notarangelo, Luigi D.] Harvard Univ, Sch Med, Childrens Hosp Boston, Manton Ctr Orphan Dis Res, Boston, MA 02115 USA. [Notarangelo, Luigi D.] Harvard Univ, Sch Med, Div Immunol, Childrens Hosp Boston, Boston, MA 02115 USA. RP Casanova, JL (reprint author), Rockefeller Univ, Rockefeller Univ Hosp, St Giles Lab Human Genet Infect Dis, Rockefeller Branch, New York, NY 10065 USA. EM jean-laurent.casanova@rockefeller.edu RI Notarangelo, Luigi/F-9718-2016 OI Notarangelo, Luigi/0000-0002-8335-0262 FU Rockefeller University; Institut National de la Sante et de la Recherche Medicale; University Paris Descartes; St. Giles Foundation; Rockefeller University Center for Clinical and Translational Science [UL1RR024143]; National Institute of Allergy and Infectious Diseases [1R37AI095983-01, 5R01AI089970-02]; Manton Foundation; Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH FX J.L.C. received support from the Rockefeller University, the Institut National de la Sante et de la Recherche Medicale, the University Paris Descartes, the St. Giles Foundation, the Rockefeller University Center for Clinical and Translational Science grant number UL1RR024143, and the National Institute of Allergy and Infectious Diseases grant numbers 1R37AI095983-01 and 5R01AI089970-02. L.D.N. was supported in part by the Manton Foundation. S.M.H. was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH. NR 153 TC 105 Z9 110 U1 3 U2 26 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 515 EP 528 DI 10.1016/j.immuni.2012.03.016 PG 14 WC Immunology SC Immunology GA 931ML UT WOS:000303223900002 PM 22520845 ER PT J AU Chen, E Staudt, LM Green, AR AF Chen, Edwin Staudt, Louis M. Green, Anthony R. TI Janus Kinase Deregulation in Leukemia and Lymphoma SO IMMUNITY LA English DT Review ID ACUTE LYMPHOBLASTIC-LEUKEMIA; B-CELL LYMPHOMA; JAK2 V617F MUTATION; ACUTE MEGAKARYOBLASTIC LEUKEMIA; HEMATOPOIETIC STEM-CELL; CHRONIC MYELOPROLIFERATIVE DISORDERS; JUVENILE MYELOMONOCYTIC LEUKEMIA; ACUTE MYELOID-LEUKEMIA; OF-FUNCTION MUTATIONS; POLYCYTHEMIA-VERA AB Genetic alterations affecting members of the Janus kinase (JAK) family have been discovered in a wide array of cancers and are particularly prominent in hematological malignancies. In this review, we focus on the role of such lesions in both myeloid and lymphoid tumors. Oncogenic JAK molecules can activate a myriad of canonical downstream signaling pathways as well as directly interact with chromatin in noncanonical processes, the interplay of which results in a plethora of diverse biological consequences. Deciphering these complexities is shedding unexpected light on fundamental cellular mechanisms and will also be important for improved diagnosis, identification of new therapeutic targets, and the development of stratified approaches to therapy. C1 [Staudt, Louis M.] NCI, Metab Branch, NIH, Bethesda, MD 20892 USA. [Chen, Edwin; Green, Anthony R.] Univ Cambridge, Cambridge Inst Med Res, Cambridge CB2 0XY, England. [Green, Anthony R.] Addenbrookes Hosp, Dept Haematol, Cambridge CB2 0XY, England. RP Staudt, LM (reprint author), NCI, Metab Branch, NIH, Bethesda, MD 20892 USA. EM lstaudt@mail.nih.gov; arg1000@cam.ac.uk FU Leukemia and Lymphoma Research; Kay Kendall Leukaemia Fund; NIHR Biomedical Cambridge Research Centre; Leukemia and Lymphoma Society of America; National Institutes of Health FX Research in the authors' laboratories is supported by the Leukemia and Lymphoma Research, the Kay Kendall Leukaemia Fund, the NIHR Biomedical Cambridge Research Centre and the Leukemia and Lymphoma Society of America (A.R.G.), and the National Institutes of Health (L.M.S.). NR 119 TC 49 Z9 52 U1 2 U2 14 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 529 EP 541 DI 10.1016/j.immuni.2012.03.017 PG 13 WC Immunology SC Immunology GA 931ML UT WOS:000303223900003 PM 22520846 ER PT J AU O'Shea, JJ Plenge, R AF O'Shea, John J. Plenge, Robert TI JAK and STAT Signaling Molecules in Immunoregulation and Immune-Mediated Disease SO IMMUNITY LA English DT Review ID GENOME-WIDE ASSOCIATION; REGULATORY T-CELLS; ACTIVE RHEUMATOID-ARTHRITIS; CORONARY-HEART-DISEASE; INHIBITOR TOFACITINIB CP-690,550; INFLAMMATORY-BOWEL-DISEASE; PRIMARY BILIARY-CIRRHOSIS; TRANSCRIPTION FACTOR; IN-VIVO; SUSCEPTIBILITY LOCI AB The discovery of the Janus kinase (JAK)-signal transducer and activator of transcripton (STAT) signaling pathway, a landmark in cell biology, provided a simple mechanism for gene regulation that dramatically advanced our understanding of the action of hormones, interferons, colony-stimulating factors, and interleukins. As we learn more about the complexities of immune responses, new insights into the functions of this pathway continue to be revealed, aided by technology that permits genome-wide views. As we celebrate the 20th anniversary of the discovery of this paradigm in cell signaling, it is particularly edifying to see how this knowledge has rapidly been translated to human immune disease. Not only have genome-wide association studies demonstrated that this pathway is highly relevant to human autoimmunity, but targeting JAKs is now a reality in immune-mediated disease. C1 [O'Shea, John J.] NIAMSD, Mol Immunol & Inflammat Branch, NIH, Bethesda, MD 20892 USA. [Plenge, Robert] Brigham & Womens Hosp, Div Genet, Div Rheumatol Allergy & Immunol, Boston, MA 02115 USA. [Plenge, Robert] Broad Inst & Harvard, Cambridge, MA 02142 USA. RP O'Shea, JJ (reprint author), NIAMSD, Mol Immunol & Inflammat Branch, NIH, Bethesda, MD 20892 USA. EM osheajo@mail.nih.gov FU Pfizer FX J.J.O'S. and National Institutes of Health (NIH) hold patents related to targeting JAKs as targets for immunomodulatory agents and have a Collaborative Research Agreement and Development Award with Pfizer. NR 110 TC 254 Z9 268 U1 7 U2 64 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 542 EP 550 DI 10.1016/j.immuni.2012.03.014 PG 9 WC Immunology SC Immunology GA 931ML UT WOS:000303223900004 PM 22520847 ER PT J AU Jin, TC Perry, A Jiang, JS Smith, P Curry, JA Unterholzner, L Jiang, ZZ Horvath, G Rathinam, VA Johnstone, RW Hornung, V Latz, E Bowie, AG Fitzgerald, KA Xiao, TS AF Jin, Tengchuan Perry, Andrew Jiang, Jiansheng Smith, Patrick Curry, James A. Unterholzner, Leonie Jiang, Zhaozhao Horvath, Gabor Rathinam, Vijay A. Johnstone, Ricky W. Hornung, Veit Latz, Eicke Bowie, Andrew G. Fitzgerald, Katherine A. Xiao, T. Sam TI Structures of the HIN Domain: DNA Complexes Reveal Ligand Binding and Activation Mechanisms of the AIM2 Inflammasome and IFI16 Receptor SO IMMUNITY LA English DT Article ID INNATE IMMUNE SENSOR; X-RAY-DIFFRACTION; RIG-I; INTRACELLULAR DNA; FRANCISELLA-TULARENSIS; PATTERN-RECOGNITION; CYTOPLASMIC DNA; RNA RECOGNITION; DENDRITIC CELLS; ATOMIC-LEVEL AB Recognition of DNA by the innate immune system is central to antiviral and antibacterial defenses, as well as an important contributor to autoimmune diseases involving self DNA. AIM2 (absent in melanoma 2) and IFI16 (interferon-inducible protein 16) have been identified as DNA receptors that induce inflammasome formation and interferon production, respectively. Here we present the crystal structures of their HIN domains in complex with double-stranded (ds) DNA. Non-sequence-specific DNA recognition is accomplished through electrostatic attraction between the positively charged HIN domain residues and the dsDNA sugar-phosphate backbone. An intramolecular complex of the AIM2 Pyrin and HIN domains in an autoinhibited state is liberated by DNA binding, which may facilitate the assembly of inflammasomes along the DNA staircase. These findings provide mechanistic insights into dsDNA as the activation trigger and oligomerization platform for the assembly of large innate signaling complexes such as the inflammasomes. C1 [Jin, Tengchuan; Perry, Andrew; Jiang, Jiansheng; Smith, Patrick; Curry, James A.; Xiao, T. Sam] NIAID, Struct lmmunobiol Unit, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Unterholzner, Leonie; Bowie, Andrew G.] Trinity Coll Dublin, Trin Biomed Sci Inst, Sch Biochem & Immunol, Dublin 2, Ireland. [Jiang, Zhaozhao; Rathinam, Vijay A.; Latz, Eicke; Fitzgerald, Katherine A.] Univ Massachusetts, Sch Med, Div Infect Dis & Immunol, Worcester, MA 01605 USA. [Hornung, Veit] Univ Bonn, Univ Hosp, Inst Clin Chem & Pharmacol, Unit Clin Biochem, D-53127 Bonn, Germany. [Johnstone, Ricky W.] Peter MacCallum Canc Inst, Canc Therapeut Program, Gene Regulat Lab, Melbourne, Vic 3002, Australia. [Johnstone, Ricky W.] Univ Melbourne, Sir Peter MacCallum Dept Oncol, Parkville, Vic 3054, Australia. RP Xiao, TS (reprint author), NIAID, Struct lmmunobiol Unit, Immunol Lab, NIH, Bethesda, MD 20892 USA. EM xiaot@niaid.nih.gov RI Xiao, Tsan/A-8590-2010; Xiao, Tsan/I-7616-2013; Jin, Tengchuan/B-5883-2014; Horvath, Gabor/J-3917-2014; Latz, Eicke/H-3951-2014; Hornung, Veit/C-3565-2012 OI Unterholzner, Leonie/0000-0001-9824-692X; Xiao, Tsan/0000-0001-9688-475X; Jin, Tengchuan/0000-0002-1395-188X; Horvath, Gabor/0000-0003-0309-595X; Latz, Eicke/0000-0003-1488-5666; Bowie, Andrew/0000-0001-5316-4373; Hornung, Veit/0000-0002-4150-194X FU Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH; Science Foundation Ireland [07/IN1/B934]; NIH [AI083713, AI067497]; European Research Council [ERC-2009-StG 243046]; DFG [SFB704, SFB670]; NHMRC; Susan G. Komen Breast Cancer Foundation; Prostate Cancer Foundation of Australia; Cancer Council Victoria; Leukemia Foundation of Australia; Victorian Breast Cancer Research Consortium; Victorian Cancer Agency; NERCE FX We thank the beam line scientists at the Argonne National Laboratory GM/CA-CAT (Argonne, IL) and the Brookhaven National Laboratory (Upton, NY) for their support on data collection. The authors would like to thank A. Mian from LI, NIAID for technical support. We thank R. Schwartz, M. Lenardo, and D. Margulies for helpful discussions. We thank D.E. Anderson at the Mass Spectrometry facility of NIDDK for technical support. T.S.X. is supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH. A.G.B. and L.U. are supported by Science Foundation Ireland (07/IN1/B934). K.A.F. is supported by NIH grants AI083713 and AI067497. E.L. is supported by NIH grant AI067497. V.H. is supported by the European Research Council (ERC-2009-StG 243046) and the DFG (SFB704 and SFB670). R.W.J. is a Principal Research Fellow of the National Health and Medical Research Council of Australia (NHMRC) and supported by NHMRC Program and Project Grants, the Susan G. Komen Breast Cancer Foundation, the Prostate Cancer Foundation of Australia, Cancer Council Victoria, The Leukemia Foundation of Australia, Victorian Breast Cancer Research Consortium, and Victorian Cancer Agency. V.A.R. is supported by a NERCE Postdoctoral fellowship. NR 64 TC 149 Z9 154 U1 2 U2 19 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 561 EP 571 DI 10.1016/j.immuni.2012.02.014 PG 11 WC Immunology SC Immunology GA 931ML UT WOS:000303223900009 PM 22483801 ER PT J AU Lin, JX Li, P Liu, DL Jin, HT He, JP Rasheed, MAU Rochman, Y Wang, L Cui, KR Liu, CY Kelsall, BL Ahmed, R Leonard, WJ AF Lin, Jian-Xin Li, Peng Liu, Delong Jin, Hyun Tak He, Jianping Rasheed, Mohammed Ata Ur Rochman, Yrina Wang, Lu Cui, Kairong Liu, Chengyu Kelsall, Brian L. Ahmed, Rafi Leonard, Warren J. TI Critical Role of STAT5 Transcription Factor Tetramerization for Cytokine Responses and Normal Immune Function SO IMMUNITY LA English DT Article ID T-CELLS; IL-2 RECEPTOR; DNA-BINDING; GENE-EXPRESSION; PROMOTER; DIMERS; ALPHA; DIFFERENTIATION; PROLIFERATION; SEQUENCE AB Cytokine-activated STAT proteins dimerize and bind to high-affinity motifs, and N-terminal domain-mediated oligomerization of dimers allows tetramer formation and binding to low-affinity tandem motifs, but the functions of dimers versus tetramers are unknown. We generated Stat5a-Stat5b double knockin (DKI) N-domain mutant mice in which STAT5 proteins form dimers but not tetramers, identified cytokine-regulated genes whose expression required STAT5 tetramers, and defined dimer versus tetramer consensus motifs. Whereas Stat5-deficient mice exhibited perinatal lethality, DKI mice were viable; thus, STAT5 dimers were sufficient for survival. Nevertheless, STAT5 DKI mice had fewer CD4(+)CD25(+) T cells, NK cells, and CD8(+) T cells, with impaired cytokine-induced and homeostatic proliferation of CD8(+) T cells. Moreover, DKI CD8(+) T cell proliferation after viral infection was diminished and DKI Treg cells did not efficiently control colitis. Thus, tetramerization of STAT5 is critical for cytokine responses and normal immune function, establishing a critical role for STAT5 tetramerization in vivo. C1 [Lin, Jian-Xin; Li, Peng; Rochman, Yrina; Wang, Lu; Cui, Kairong; Leonard, Warren J.] NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. [Liu, Delong] Ctr Informat Technol, Math & Stat Comp Lab, Bethesda, MD 20892 USA. [Jin, Hyun Tak; Rasheed, Mohammed Ata Ur; Ahmed, Rafi] Emory Univ, Sch Med, Emory Vaccine Ctr, Atlanta, GA 30322 USA. [He, Jianping; Kelsall, Brian L.] NIAID, Mucosal Immun Sect, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. [Liu, Chengyu] NHLBI, NIH, Bethesda, MD 20892 USA. RP Leonard, WJ (reprint author), NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. EM wjl@helix.nih.gov RI Rasheed, Mohammed/N-2749-2013 FU Division of Intramural Research, NHLBI, NIH, Bethesda, MD FX We thank N. Raghavachari (NHLBI) for Affymetrix Chip assay, R. Spolski (NHLBI), R.H. Schwartz, K.-T. Jeang (NIAID), K. Zhao (NHLBI), and P.J. Munson (CIT) for critical comments, P.J. Munson (CIT) for his input in Affymetrix analysis, J. Chen (Hematology Branch), R. Spolski, W. Liao, and C. Robinson for experimental help, other members in the Leonard lab for suggestions, and C. Robinson for maintaining the mouse colony. We thank C. Wu, S. Adhya, and S. Simmons for valuable discussions. This work was supported in part by the Division of Intramural Research, NHLBI, NIH, Bethesda, MD. NR 43 TC 54 Z9 59 U1 0 U2 18 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 586 EP 599 DI 10.1016/j.immuni.2012.02.017 PG 14 WC Immunology SC Immunology GA 931ML UT WOS:000303223900011 PM 22520852 ER PT J AU Liu, DF Peterson, ME Long, EO AF Liu, Dongfang Peterson, Mary E. Long, Eric O. TI The Adaptor Protein Crk Controls Activation and Inhibition of Natural Killer Cells SO IMMUNITY LA English DT Article ID MHC CLASS-I; CHRONIC VIRAL-INFECTION; COMPLEX CLASS-I; IMMUNE SYNAPSES; ACTIN CYTOSKELETON; TYROSINE PHOSPHORYLATION; LIVING CELLS; T-CELLS; RECEPTORS; REORGANIZATION AB Natural killer (NK) cell inhibitory receptors recruit tyrosine phosphatases to prevent activation, induce phosphorylation and dissociation of the small adaptor Crk from cytoskeleton scaffold complexes, and maintain NK cells in a state of responsiveness to subsequent activation events. How Crk contributes to inhibition is unknown. We imaged primary NK cells over lipid bilayers carrying IgG1 Fc to stimulate CD16 and human leukocyte antigen (HLA)-E to inhibit through receptor CD94-NKG2A. HLA-E alone induced Crk phosphorylation in NKG2A(+) NK cells. At activating synapses with Fc alone, Crk was required for the movement of Fc microclusters and their ability to trigger activation signals. At inhibitory synapses, HLA-E promoted central accumulation of both Fc and phosphorylated Crk and blocked the Fc-induced buildup of F-actin. We propose a unified model for inhibitory receptor function: Crk phosphorylation prevents essential Crk-dependent activation signals and blocks F-actin network formation, thereby reducing constraints on subsequent engagement of activation receptors. C1 [Liu, Dongfang; Peterson, Mary E.; Long, Eric O.] NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. RP Long, EO (reprint author), NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. EM elong@nih.gov RI Long, Eric/G-5475-2011 OI Long, Eric/0000-0002-7793-3728 FU National Institutes of Health, National Institute of Allergy and Infectious Diseases FX We thank J. Brzostowski, S. Rajagopalan, and P. Sun for advice and help, D. Geraghty and M. Robertson for cell lines, and Y. Ohba for the CrkL biosensor. This work has been supported by the Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Diseases. NR 51 TC 32 Z9 32 U1 0 U2 8 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 600 EP 611 DI 10.1016/j.immuni.2012.03.007 PG 12 WC Immunology SC Immunology GA 931ML UT WOS:000303223900012 PM 22464172 ER PT J AU Valdez, PA Vithayathil, PJ Janelsins, BM Shaffer, AL Williamson, PR Datta, SK AF Valdez, Patricia A. Vithayathil, Paul J. Janelsins, Brian M. Shaffer, Arthur L. Williamson, Peter R. Datta, Sandip K. TI Prostaglandin E2 Suppresses Antifungal Immunity by Inhibiting Interferon Regulatory Factor 4 Function and Interleukin-17 Expression in T Cells SO IMMUNITY LA English DT Article ID CHRONIC MUCOCUTANEOUS CANDIDIASIS; CRYPTOCOCCUS-NEOFORMANS INFECTION; ARYL-HYDROCARBON RECEPTOR; HYPER-IGE SYNDROME; ROR-GAMMA-T; TH17 RESPONSES; HELPER-CELLS; HOST-DEFENSE; BETA-GLUCAN; CYCLIC-AMP AB T helper 17 (Th17) cells play an important role in mucosal host defense through production of the signature cytokines IL-17 and IL-22. Prostaglandin E2 (PGE2) has been shown to enhance IL-17 production by mature Th17 cells. However, when present during Th17 cell differentiation, we found that PGE2 inhibited the transcription factor IRF4 and suppressed production of IL-17 but not IL-22. We show that IRF4 was required for IL-17 expression but inhibited IL-22 expression, highlighting the potential for discordant regulation of these two cytokines in Th17 cells. The pathogenic fungus Cryptococcus neoformans produces PGE2, and we found that it uses PGE2- and IRF4-dependent mechanisms to specifically inhibit induction of IL-17 during Th17 cell differentiation. Blockade of host PGE2 during infection led to increased IL-17 production from CD4(+) T cells and increased survival of mice. These findings suggest that host-or pathogen-derived PGE2 can act directly on Th17 cells during differentiation to inhibit IL-17-dependent antimicrobial responses. C1 [Valdez, Patricia A.; Vithayathil, Paul J.; Janelsins, Brian M.; Datta, Sandip K.] NIAID, Bacterial Pathogenesis Unit, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Shaffer, Arthur L.] NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Williamson, Peter R.] NIAID, Translat Mycol Unit, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. RP Datta, SK (reprint author), NIAID, Bacterial Pathogenesis Unit, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. EM dattas@niaid.nih.gov OI Datta, Sandip/0000-0003-0243-7815 FU NIH, NIAID FX We thank R. Munford (NIAID) for discussion and comments on the manuscript and members of the J. O'Shea (NIAMS) and W. Leonard (NHLBI) laboratories for assistance in identifying ChIP binding regions. This work was supported by the Intramural Research Program of the NIH, NIAID. NR 56 TC 45 Z9 45 U1 0 U2 6 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1074-7613 J9 IMMUNITY JI Immunity PD APR 20 PY 2012 VL 36 IS 4 BP 668 EP 679 DI 10.1016/j.immuni.2012.02.013 PG 12 WC Immunology SC Immunology GA 931ML UT WOS:000303223900018 PM 22464170 ER PT J AU Gellhorn, AC Chan, L Martin, B Friedly, J AF Gellhorn, Alfred Campbell Chan, Leighton Martin, Brook Friedly, Janna TI Management Patterns in Acute Low Back Pain The Role of Physical Therapy SO SPINE LA English DT Article DE physical therapy; low back pain; epidural steroid injection; lumbar surgery; Medicare; epidemiology ID RANDOMIZED CLINICAL-TRIAL; PRIMARY-CARE; CLAIMS DATA; BED REST; OUTCOMES; RECURRENCE; GUIDELINES; SPECIALTY; REHABILITATION; CLASSIFICATION AB Study Design. Retrospective cohort study. Objective. To evaluate the relationship between early physical therapy (PT) for acute low back pain and subsequent use of lumbosacral injections, lumbar surgery, and frequent physician office visits for low back pain. Summary of Background Data. Wide practice variations exist in the treatment of acute low back pain. PT has been advocated as an effective treatment in this setting although disagreement exists regarding its purported benefits. Methods. A national 20% sample of the Centers for Medicare and Medicaid Services physician outpatient billing claims was analyzed. Patients were selected who received treatment for low back pain between 2003 and 2004 (n = 439,195). To exclude chronic low back conditions, patients were excluded if they had a prior visit for back pain, lumbosacral injection, or lumbar surgery within the previous year. Main outcome measures were rates of lumbar surgery, lumbosacral injections, and frequent physician office visits for low back pain during the following year. Results. Based on logistic regression analysis, the adjusted odds ratio for undergoing surgery in the group of enrollees that received PT in the acute phase (<4 weeks) compared to those receiving PT in the chronic phase (>3 months) was 0.38 (95% confidence interval [CI], 0.360.41), adjusting for age, sex, diagnosis, treating physician specialty, and comorbidity. The adjusted odds ratio for receiving a lumbosacral injection in the group receiving PT in the acute phase was 0.46 (95% CI, 0.44-0.49), and the adjusted odds ratio for frequent physician office usage in the group receiving PT in the acute phase was 0.47 (95% CI, 0.44-0.50). Conclusion. There was a lower risk of subsequent medical service usage among patients who received PT early after an episode of acute low back pain relative to those who received PT at later times. Medical specialty variations exist regarding early use of PT, with potential underutilization among generalist specialties. C1 [Gellhorn, Alfred Campbell; Friedly, Janna] Univ Washington, Sch Med, Dept Rehabil Med, Seattle, WA 98195 USA. [Chan, Leighton] NIH, Dept Rehabil Med, Ctr Clin, Bethesda, MD 20892 USA. [Martin, Brook] Univ Washington, Ctr Cost & Outcomes Res, Seattle, WA 98195 USA. RP Gellhorn, AC (reprint author), 525 W Chester Pike,Suite 203, Havertown, PA 19083 USA. EM gellhorn@me.com FU NIH FX This research was supported by the NIH Intramural Research Program. The manuscript submitted does not contain information about medical device(s)/drug(s). NR 41 TC 29 Z9 29 U1 0 U2 10 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0362-2436 EI 1528-1159 J9 SPINE JI SPINE PD APR 20 PY 2012 VL 37 IS 9 BP 775 EP 782 DI 10.1097/BRS.0b013e3181d79a09 PG 8 WC Clinical Neurology; Orthopedics SC Neurosciences & Neurology; Orthopedics GA 929MB UT WOS:000303067600016 PM 21099735 ER PT J AU Chen, ZG Eggerman, TL Bocharov, AV Baranova, IN Vishnyakova, TG Kurlander, RJ Csako, G Patterson, AP AF Chen, Zhigang Eggerman, Thomas L. Bocharov, Alexander V. Baranova, Irina N. Vishnyakova, Tatyana G. Kurlander, Roger J. Csako, Gyorgy Patterson, Amy P. TI Hypermutation of ApoB mRNA by Rat APOBEC-1 Overexpression Mimics APOBEC-3 Hypermutation SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE APOBEC-1; APOBEC-3G; hypermutation; editing; HBV ID HUMAN-IMMUNODEFICIENCY-VIRUS; DNA CYTIDINE DEAMINASE; EDITING PROTEIN; MOLECULAR-CLONING; HIV-1; VIF; RESTRICTION; COMPONENTS; INFECTION; IMMUNITY AB APOBEC-3 proteins induce C-to-U hypermutations in the viral genome of various viruses and have broad antiviral activity. Generally, only a small proportion of viral genomes (< 10(-2)) are hypermutated by APOBEC-3s, but often many cytidines (up to 40%) are converted into uridine. The mechanism of this unique selective hypermutation remains unknown. We found that rat APOBEC-1 overexpression had a hypermutation pattern similar to that of APOBEC-3s on its substrate apolipoprotein B (apoB) mRNA. Transient plasmid transfection of rat APOBEC-1 resulted in 0.4% and 1.8% hypermutations with apoB mRNA in HepG2 and McA7777 cells, respectively. The low frequency of hypermutated apoB mRNA targets was enriched by differential DNA denaturation PCR at 72-76 degrees C, with hypermutation levels increasing up to 67%. Up to 69.6% of cytidines in HepG2 and up to 75.5% of cytidines in McA7777 cells were converted into uridines in the hypermutated apoB mRNA. When rat APOBEC-1 was overexpressed by adenovirus, the hypermutation frequency of apoB mRNA increased from 0.4% to similar to 20% and was readily detected by regular PCR. However, this higher expression efficiency only increased the frequency of hypermutation, not the number of affected cytidines in hypermutated targets. Rat APOBEC-1 hypermutation was modulated by cofactors and eliminated by an E181Q mutation, indicating the role of cofactors in hypermutation. The finding of an APOBEC-3 hypermutation pattern with rat APOBEC-1 suggests that cofactors could also be involved in APOBEC-3 hypermutation. Using hepatitis B virus hypermutation, we found that KSRP increased APOBEC-3C and APOBEC-3B hypermutation. These data show that, like rat APOBEC-1 hypermutation, cellular factors may play a regulatory role in APOBEC-3 hyperrnutation. Published by Elsevier Ltd. C1 [Patterson, Amy P.] NIH, Off Sci Policy, Off Director, Bethesda, MD 20892 USA. [Chen, Zhigang; Eggerman, Thomas L.; Bocharov, Alexander V.; Baranova, Irina N.; Vishnyakova, Tatyana G.; Kurlander, Roger J.; Csako, Gyorgy; Patterson, Amy P.] NIH, Dept Lab Med, Ctr Clin, Bethesda, MD 20892 USA. [Eggerman, Thomas L.] NIDDK, Div Diabet Endocrinol & Metab Dis, NIH, Bethesda, MD 20892 USA. RP Patterson, AP (reprint author), NIH, Off Sci Policy, Off Director, 6705 Rockledge Dr,Suite 750, Bethesda, MD 20892 USA. EM pattersa@od.nih.gov FU Intramural NIH HHS [Z99 CL999999, Z99 DK999999] NR 37 TC 4 Z9 4 U1 0 U2 1 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD APR 20 PY 2012 VL 418 IS 1-2 BP 65 EP 81 DI 10.1016/j.jmb.2012.02.005 PG 17 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 926KX UT WOS:000302831000007 PM 22326345 ER PT J AU Chen, J Lippincott-Schwartz, J Liu, J AF Chen, Jing Lippincott-Schwartz, Jennifer Liu, Jian TI Intracellular Spatial Localization Regulated by the Microtubule Network SO PLOS ONE LA English DT Article ID DROSOPHILA NEUROBLASTS; CELL-DIVISION; CYTOPLASMIC DYNEIN; CORTICAL POLARITY; SPINDLE POLES; MESSENGER-RNA; LIVING CELLS; PROTEIN; DORSAL; EMBRYOS AB The commonly recognized mechanisms for spatial regulation inside the cell are membrane-bounded compartmentalization and biochemical association with subcellular organelles. We use computational modeling to investigate another spatial regulation mechanism mediated by the microtubule network in the cell. Our results demonstrate that the mitotic spindle can impose strong sequestration and concentration effects on molecules with binding affinity for microtubules, especially dynein-directed cargoes. The model can recapitulate the essence of three experimental observations on distinct microtubule network morphologies: the sequestration of germ plasm components by the mitotic spindles in the Drosophila syncytial embryo, the asymmetric cell division initiated by the time delay in centrosome maturation in the Drosophila neuroblast, and the diffusional block between neighboring energids in the Drosophila syncytial embryo. Our model thus suggests that the cell cycle-dependent changes in the microtubule network are critical for achieving different spatial regulation effects. The microtubule network provides a spatially extensive docking platform for molecules and gives rise to a "structured cytoplasm", in contrast to a free and fluid environment. C1 [Chen, Jing; Liu, Jian] NHLBI, NIH, Bethesda, MD 20892 USA. [Lippincott-Schwartz, Jennifer] NICHHD, NIH, Bethesda, MD 20892 USA. RP Chen, J (reprint author), NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA. EM Jian.Liu@nih.gov RI Chen, Jing/D-4845-2016 OI Chen, Jing/0000-0001-6321-0505 FU National Heart, Lung and Blood Institute at National Institutes of Health (NIH); National Institute of Child Health and Human Development at NIH FX Dr. Chen and Dr. Liu were supported by the Intramural Research Program of National Heart, Lung and Blood Institute at National Institutes of Health (NIH); Dr. Lippincott-Schwartz was supported by the Intramural Research Program of National Institute of Child Health and Human Development at NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 8 Z9 8 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 19 PY 2012 VL 7 IS 4 AR e34919 DI 10.1371/journal.pone.0034919 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TL UT WOS:000305336200013 PM 22532834 ER PT J AU DeBaun, MR Sarnaik, SA Rodeghier, MJ Minniti, CP Howard, TH Iyer, RV Inusa, B Telfer, PT Kirby-Allen, M Quinn, CT Bernaudin, F Airewele, G Woods, GM Panepinto, JA Fuh, B Kwiatkowski, JK King, AA Rhodes, MM Thompson, AA Heiny, ME Redding-Lallinger, RC Kirkham, FJ Sabio, H Gonzalez, CE Saccente, SL Kalinyak, KA Strouse, JJ Fixler, JM Gordon, MO Miller, JP Noetzel, MJ Ichord, RN Casella, JF AF DeBaun, Michael R. Sarnaik, Sharada A. Rodeghier, Mark J. Minniti, Caterina P. Howard, Thomas H. Iyer, Rathi V. Inusa, Baba Telfer, Paul T. Kirby-Allen, Melanie Quinn, Charles T. Bernaudin, Francoise Airewele, Gladstone Woods, Gerald M. Panepinto, Julie Ann Fuh, Beng Kwiatkowski, Janet K. King, Allison A. Rhodes, Melissa M. Thompson, Alexis A. Heiny, Mark E. Redding-Lallinger, Rupa C. Kirkham, Fenella J. Sabio, Hernan Gonzalez, Corina E. Saccente, Suzanne L. Kalinyak, Karen A. Strouse, John J. Fixler, Jason M. Gordon, Mae O. Miller, J. Phillip Noetzel, Michael J. Ichord, Rebecca N. Casella, James F. TI Associated risk factors for silent cerebral infarcts in sickle cell anemia: low baseline hemoglobin, sex, and relative high systolic blood pressure SO BLOOD LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; TRANSFUSION SIT TRIAL; PULMONARY-HYPERTENSION; YOUNG-CHILDREN; DISEASE; BRAIN; STROKE; ABNORMALITIES; MRI; COMPLICATIONS AB The most common form of neurologic injury in sickle cell anemia (SCA) is silent cerebral infarction (SCI). In the Silent Cerebral Infarct Multi-Center Clinical Trial, we sought to identify risk factors associated with SCI. In this cross-sectional study, we evaluated the clinical history and baseline laboratory values and performed magnetic resonance imaging of the brain in participants with SCA (HbSS or HbS beta degrees thalassemia) between the ages of 5 and 15 years with no history of overt stroke or seizures. Neuroradiology and neurology committees adjudicated the presence of SCI. SCIs were diagnosed in 30.8% (251 of 814) participants who completed all evaluations and had valid data on all prespecified demographic and clinical covariates. The mean age of the participants was 9.1 years, with 413 males (50.7%). In a multivariable logistic regression analysis, lower baseline hemoglobin concentration (P < .001), higher baseline systolic blood pressure (P = .018), and male sex (P = .030) were statistically significantly associated with an increased risk of an SCI. Hemoglobin concentration and systolic blood pressure are risk factors for SCI in children with SCA and may be therapeutic targets for decreasing the risk of SCI. This study is registered at www.clinicaltrials.gov as #NCT00072761. (Blood. 2012; 19(16):3684-3690) C1 [DeBaun, Michael R.] Vanderbilt Univ, Dept Pediat, Div Hematol Oncol, Nashville, TN 37232 USA. [Sarnaik, Sharada A.] Wayne State Univ, Dept Pediat, Div Hematol Oncol, Detroit, MI 48202 USA. [Rodeghier, Mark J.] Rodeghier Consultants, Chicago, IL USA. [Minniti, Caterina P.] NHLBI, NIH, Bethesda, MD 20892 USA. [Howard, Thomas H.] Univ Alabama Birmingham, Div Hematol Oncol, Dept Pediat, Birmingham, AL 35294 USA. [Iyer, Rathi V.] Univ Mississippi, Med Ctr, Dept Pediat, Div Hematol Oncol, Jackson, MS 39216 USA. [Inusa, Baba] St Thomas Hosp Natl Hlth Serv Trust, Evelina Childrens Hosp, Dept Paediat, London, England. [Telfer, Paul T.] Royal London Hosp, Dept Pediat Hematol, London E1 1BB, England. [Kirby-Allen, Melanie] Univ Toronto, Hosp Sick Children, Toronto, ON M5G 1X8, Canada. [Quinn, Charles T.; Kalinyak, Karen A.] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Cincinnati, OH USA. [Quinn, Charles T.; Kalinyak, Karen A.] Univ Cincinnati, Cincinnati, OH USA. [Bernaudin, Francoise] Hop Intercommunal Creteil, Dept Pediat, Creteil, France. [Airewele, Gladstone] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Woods, Gerald M.] Univ Missouri, Dept Pediat, Kansas City, MO 64110 USA. [Panepinto, Julie Ann] Med Coll Wisconsin, Dept Pediat, Milwaukee, WI 53226 USA. [Fuh, Beng] Brody Sch Med, Dept Pediat, Greenville, NC USA. [Kwiatkowski, Janet K.; Ichord, Rebecca N.] Univ Penn, Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA. [King, Allison A.; Noetzel, Michael J.] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA. [Rhodes, Melissa M.] Ohio State Univ, Dept Pediat, Columbus, OH 43210 USA. [Thompson, Alexis A.] Northwestern Univ, Dept Pediat, Chicago, IL 60611 USA. [Heiny, Mark E.] Indiana Univ Purdue Univ, Dept Pediat, Indianapolis, IN 46202 USA. [Redding-Lallinger, Rupa C.] Univ N Carolina, Dept Pediat, Chapel Hill, NC USA. [Kirkham, Fenella J.] UCL, Inst Child Hlth, Neurosci Unit, London, England. [Sabio, Hernan] Wake Forest Univ Hlth Sci, Dept Pediat, Winston Salem, NC USA. [Gonzalez, Corina E.] Georgetown Univ Hosp, Dept Pediat, Washington, DC 20007 USA. [Saccente, Suzanne L.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. [Strouse, John J.; Casella, James F.] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA. [Fixler, Jason M.] Sinai Hosp, Sch Med, Baltimore, MD 21215 USA. [Gordon, Mae O.] Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USA. [Miller, J. Phillip] Washington Univ, Sch Med, Div Biostat, St Louis, MO 63110 USA. [Noetzel, Michael J.] Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA. RP DeBaun, MR (reprint author), Vanderbilt Univ, Dept Pediat, Monroe Carell Jr Childrens Hosp Vanderbilt, Sch Med, 2200 Childrens Way, Nashville, TN 37232 USA. EM m.debaun@vanderbilt.edu RI Kirkham, Fenella/C-2442-2009; Quinn, Charles/J-6842-2012; OI Kirkham, Fenella/0000-0002-2443-7958; Quinn, Charles/0000-0002-2372-2175; King, Allison/0000-0002-1951-6176 FU National Institutes of Health [U01-NS42804] FX This work was supported by the National Institutes of Health (grant U01-NS42804; M.R.D.). NR 33 TC 60 Z9 60 U1 0 U2 3 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 19 PY 2012 VL 119 IS 16 BP 3684 EP 3690 DI 10.1182/blood-2011-05-349621 PG 7 WC Hematology SC Hematology GA 959AM UT WOS:000305282100011 PM 22096242 ER PT J AU Deffenbacher, KE Iqbal, J Sanger, W Shen, YL Lachel, C Liu, ZF Liu, YY Lim, MS Perkins, SL Fu, K Smith, L Lynch, J Staudt, LM Rimsza, LM Jaffe, E Rosenwald, A Ott, GK Delabie, J Campo, E Gascoyne, RD Cairo, MS Weisenburger, DD Greiner, TC Gross, TG Chan, WC AF Deffenbacher, Karen E. Iqbal, Javeed Sanger, Warren Shen, Yulei Lachel, Cynthia Liu, Zhongfeng Liu, Yanyan Lim, Megan S. Perkins, Sherrie L. Fu, Kai Smith, Lynette Lynch, James Staudt, Louis M. Rimsza, Lisa M. Jaffe, Elaine Rosenwald, Andreas Ott, German K. Delabie, Jan Campo, Elias Gascoyne, Randy D. Cairo, Mitchell S. Weisenburger, Dennis D. Greiner, Timothy C. Gross, Thomas G. Chan, Wing C. TI Molecular distinctions between pediatric and adult mature B-cell non-Hodgkin lymphomas identified through genomic profiling SO BLOOD LA English DT Article ID CHEMOTHERAPY PLUS RITUXIMAB; BURKITTS-LYMPHOMA; GENE-EXPRESSION; CHROMOSOMAL ALTERATIONS; COPY NUMBER; ADOLESCENTS; CHILDREN; PROTEIN; SURVIVAL; TRIAL AB Burkitt lymphoma (BL) predominates in pediatric patients, whereas diffuse large B-cell lymphoma (DLBCL) is uncommon. In contrast to adults, BL and DLBCL are treated similarly in children and both entities have superior outcomes in children compared with adults. Gene expression profiling (GEP) and miRNA expression profiling clearly differentiated pediatric DLBCL from BL, forming distinct clusters regardless of patient age. However, pathway analysis of GEP data identified minor differences between corresponding pediatric and adult tumors. Predominance (6:1) of the germinal center B-cell subtype to activated B-cell subtype was found among pediatric DLBCL. Two cases were molecularly classified as primary mediastinal B-cell lymphoma. We observed frequent abnormalities in 8q24 in pediatric DLBCL, including MYC rearrangement in 31% (5 of 16) and gain or amplification in 50% (6 of 12) nonrearranged cases. MYC rearrangement was present in 96% (23 of 24) BL cases. Array-based CGH analysis identified abnormalities that are shared between adult and pediatric DLBCL (+12q15, +19q13, -6q), and abnormalities unique to the pediatric cases (-4p14, -19q13.32, +16p11.2), suggesting distinct pathogenetic mechanisms relative to age. Elucidation of the underlying target genes may provide insight into factors that modulate outcome and could provide potential novel therapeutic targets with less toxicity for pediatric patients with B-cell non-Hodgkin lymphoma. (Blood. 2012;119(16):3757-3766) C1 [Chan, Wing C.] Univ Nebraska Med Ctr, Ctr Res Lymphoma & Leukemia, Dept Pathol & Microbiol, Omaha, NE 68198 USA. [Sanger, Warren] Univ Nebraska Med Ctr, Munroe Meyer Inst, Omaha, NE 68198 USA. [Lim, Megan S.] Univ Michigan Hlth Syst, Dept Pathol, Ann Arbor, MI USA. [Perkins, Sherrie L.] Univ Utah, Dept Pathol, Salt Lake City, UT USA. [Smith, Lynette; Lynch, James] Univ Nebraska Med Ctr, Dept Biostat, Omaha, NE 68198 USA. [Staudt, Louis M.] NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Rimsza, Lisa M.] Univ Arizona, Dept Pathol, Tucson, AZ USA. [Jaffe, Elaine] NCI, Pathol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Rosenwald, Andreas] Univ Wurzburg, Dept Pathol, D-8700 Wurzburg, Germany. [Ott, German K.] Robert Bosch Krankenhaus, Dept Clin Pathol, Stuttgart, Germany. [Delabie, Jan] Univ Oslo, Norwegian Radium Hosp, Dept Pathol, Oslo, Norway. [Campo, Elias] Univ Barcelona, Hosp Clin, Barcelona, Spain. [Gascoyne, Randy D.] British Columbia Canc Agcy, Ctr Lymphoid Canc, Dept Pathol, Vancouver, BC V5Z 4E6, Canada. [Gascoyne, Randy D.] British Columbia Canc Agcy, Ctr Lymphoid Canc, Dept Lab Med, Vancouver, BC V5Z 4E6, Canada. [Cairo, Mitchell S.] Columbia Univ, Dept Pediat, New York, NY 10027 USA. [Cairo, Mitchell S.] Columbia Univ, Dept Med, New York, NY USA. [Cairo, Mitchell S.] Columbia Univ, Dept Pathol, New York, NY USA. [Cairo, Mitchell S.] Columbia Univ, Dept Cell Biol, New York, NY USA. [Gross, Thomas G.] Nationwide Childrens Hosp, Sect Hematol Oncol Blood & Marrow Transplant, Columbus, OH USA. RP Chan, WC (reprint author), Univ Nebraska Med Ctr, Ctr Res Lymphoma & Leukemia, Dept Pathol & Microbiol, 983135 Nebraska Med Ctr, Omaha, NE 68198 USA. EM jchan@unmc.edu OI Jaffe, Elaine/0000-0003-4632-0301; Delabie, Jan/0000-0001-5023-0689; Campo, elias/0000-0001-9850-9793 FU National Cancer Institute [5U01/CA114778]; National Institutes of Health (NIH) [U01/CA84967]; Eppley Core Grant; NIH from the INBRE of the National Center for Research Resources [P20 RR016469]; Chair's Grant [U10 CA98543-08]; Statistics and Data Center of the Children's Oncology Group from the National Cancer Institute, NIH [U10 CA98413-08] FX This work was supported in part by the National Cancer Institute (grant 5U01/CA114778), National Institutes of Health (NIH; grant U01/CA84967), and Eppley Core Grant. The University of Nebraska Medical Center Microarray Core Facility is supported in part by the NIH (grant P20 RR016469) from the INBRE Program of the National Center for Research Resources. Research is also supported in part by the Chair's Grant (grant U10 CA98543-08) and Statistics and Data Center (grant U10 CA98413-08) of the Children's Oncology Group from the National Cancer Institute, NIH. NR 47 TC 23 Z9 25 U1 1 U2 4 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 19 PY 2012 VL 119 IS 16 BP 3757 EP 3766 DI 10.1182/blood-2011-05-349662 PG 10 WC Hematology SC Hematology GA 959AM UT WOS:000305282100019 PM 22374697 ER PT J AU Vlachos, A Rosenberg, PS Atsidaftos, E Alter, BP Lipton, JM AF Vlachos, Adrianna Rosenberg, Philip S. Atsidaftos, Eva Alter, Blanche P. Lipton, Jeffrey M. TI Incidence of neoplasia in Diamond Blackfan anemia: a report from the Diamond Blackfan Anemia Registry SO BLOOD LA English DT Article ID MARROW FAILURE SYNDROMES; FANCONI-ANEMIA; NATURAL-HISTORY; CANCER; RISKS AB Diamond Blackfan anemia (DBA) is an inherited bone marrow failure syndrome characterized by red cell aplasia and congenital anomalies. A predisposition to cancer has been suggested but not quantified by case reports. The DBA Registry of North America (DBAR) is the largest established DBA patient cohort, with prospective follow-up since 1991. This report presents the first quantitative assessment of cancer incidence in DBA. Among 608 patients with 9458 person-years of follow-up, 15 solid tumors, 2 acute myeloid leukemias, and 2 cases of myelodysplastic syndrome were diagnosed at a median age of 41 years in patients who had not received a bone marrow transplant. Cancer incidence in DBA was significantly elevated. The observed-to-expected ratio for all cancers combined was 5.4 (P < .05); significant observed-to-expected ratios were 287 for myelodysplastic syndrome, 28 for acute myeloid leukemia, 36 for colon carcinoma, 33 for osteogenic sarcoma, and 12 for female genital cancers. The median survival was 56 years, and the cumulative incidence of solid tumor/leukemia was approximately 20% by age 46 years. As in Fanconi anemia and dyskeratosis congenita, DBA is both an inherited bone marrow failure syndrome and a cancer predisposition syndrome; cancer risks appear lower in DBA than in Fanconi anemia or dyskeratosis congenita. This trial was registered at www.clinicaltrials.gov as #NCT00106015. (Blood. 2012;119(16):3815-3819) C1 [Vlachos, Adrianna; Atsidaftos, Eva; Lipton, Jeffrey M.] Steven & Alexandra Cohen Childrens Med Ctr New Yo, New Hyde Pk, NY USA. [Vlachos, Adrianna; Atsidaftos, Eva; Lipton, Jeffrey M.] Feinstein Inst Med Res, Manhasset, NY USA. [Vlachos, Adrianna; Lipton, Jeffrey M.] Hofstra N Shore Long Isl Jewish Sch Med, Hempstead, NY USA. [Alter, Blanche P.] NCI, Clin Genet Branch, Div Canc Epidemiol & Genet, Natl Inst Hlth,Dept Hlth & Human Serv, Rockville, MD USA. RP Vlachos, A (reprint author), Cohen Childrens Med Ctr, 269-01 76th Ave,Rm 255, New Hyde Pk, NY 11040 USA. EM avlachos@nshs.edu FU National Heart, Lung, and Blood Institute [R01 HL 079571-07]; Centers for Disease Control and Prevention; Pediatric Cancer Foundation; National Institutes of Health and the National Cancer Institute FX This research was supported in part by grants from the National Heart, Lung, and Blood Institute (R01 HL 079571-07; A.V., E.A., and J.M.L.), the Centers for Disease Control and Prevention (A.V.), the Pediatric Cancer Foundation (J.M.L.), and the Intramural Research Program of the National Institutes of Health and the National Cancer Institute (P.S.R. and B.P.A.). NR 14 TC 70 Z9 71 U1 0 U2 7 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 19 PY 2012 VL 119 IS 16 BP 3815 EP 3819 DI 10.1182/blood-2011-08-375972 PG 5 WC Hematology SC Hematology GA 959AM UT WOS:000305282100025 PM 22362038 ER PT J AU Bender, MA Ragoczy, T Lee, J Byron, R Telling, A Dean, A Groudine, M AF Bender, M. A. Ragoczy, Tobias Lee, Jongjoo Byron, Rachel Telling, Agnes Dean, Ann Groudine, Mark TI The hypersensitive sites of the murine beta-globin locus control region act independently to affect nuclear localization and transcriptional elongation SO BLOOD LA English DT Article ID IN-VIVO; ERYTHROID-DIFFERENTIATION; GENE-TRANSCRIPTION; TARGETED DELETION; P-TEFB; REVEALS; LCR; HS3; EXPRESSION; DYNAMICS AB The beta-globin locus control region (LCR) is necessary for high-level beta-globin gene transcription and differentiation-dependent relocation of the beta-globin locus from the nuclear periphery to the central nucleoplasm and to foci of hyperphosphorylated Pol II "transcription factories" (TFys). To determine the contribution of individual LCR DNasel hypersensitive sites (HSs) to transcription and nuclear location, in the present study, we compared beta-globin gene activity and location in erythroid cells derived from mice with deletions of individual HSs, deletions of 2 HSs, and deletion of the whole LCR and found all of the HSs had a similar spectrum of activities, albeit to different degrees. Each HS acts as an independent module to activate expression in an additive manner, and this is correlated with relocation away from the nuclear periphery. In contrast, HSs have redundant activities with respect to association with TFys and the probability that an allele is actively transcribed, as measured by primary RNA transcript FISH. The limiting effect on RNA levels occurs after beta-globin genes associate with TFys, at which time HSs contribute to the amount of RNA arising from each burst of transcription by stimulating transcriptional elongation. (Blood. 2012;119(16):3820-3827) C1 [Ragoczy, Tobias; Byron, Rachel; Telling, Agnes; Groudine, Mark] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA. [Bender, M. A.] Univ Washington, Dept Pediat, Seattle, WA 98195 USA. [Bender, M. A.] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA. [Lee, Jongjoo; Dean, Ann] NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD USA. [Groudine, Mark] Univ Washington, Dept Radiat Oncol, Seattle, WA 98195 USA. RP Groudine, M (reprint author), Fred Hutchinson Canc Res Ctr, Div Basic Sci, Mailstop A3-025,1100 Fairview Ave N, Seattle, WA 98109 USA. EM markg@fhcrc.org FU National Institutes of Health [DK44746, HL065440]; intramural program of the National Diabetes and Digestive and Kidney Diseases, National Institutes of Health; Cooley's Anemia Foundation FX This work was supported by the National Institutes of Health (grants DK44746 and HL065440 to M.G.), the intramural program of the National Diabetes and Digestive and Kidney Diseases, National Institutes of Health (to A.D.), and the Cooley's Anemia Foundation (to M.A.B). NR 45 TC 17 Z9 17 U1 0 U2 5 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD APR 19 PY 2012 VL 119 IS 16 BP 3820 EP 3827 DI 10.1182/blood-2011-09-380485 PG 8 WC Hematology SC Hematology GA 959AM UT WOS:000305282100026 PM 22378846 ER PT J AU Hwang, S Maloney, NS Bruinsma, MW Goel, G Duan, EN Zhang, L Shrestha, B Diamond, MS Dani, A Sosnovtsev, SV Green, KY Lopez-Otin, C Xavier, RJ Thackray, LB Virgin, HW AF Hwang, Seungmin Maloney, Nicole S. Bruinsma, Monique W. Goel, Gautam Duan, Erning Zhang, Lei Shrestha, Bimmi Diamond, Michael S. Dani, Adish Sosnovtsev, Stanislav V. Green, Kim Y. Lopez-Otin, Carlos Xavier, Ramnik J. Thackray, Larissa B. Virgin, Herbert W. TI Nondegradative Role of Atg5-Atg12/Atg16L1 Autophagy Protein Complex in Antiviral Activity of Interferon Gamma SO CELL HOST & MICROBE LA English DT Article ID SECRETORY PATHWAY; VIRUS-INFECTION; IMMUNITY; REPLICATION; LC3; INFLAMMATION; MACROPHAGES; BIOGENESIS; LIPIDATION; ACTIVATION AB Host resistance to viral infection requires type I (alpha/beta) and II (gamma) interferon (IFN) production. Another important defense mechanism is the degradative activity of macroautophagy (herein autophagy), mediated by the coordinated action of evolutionarily conserved autophagy proteins (Atg). We show that the Atg5-Atg12/Atg16L1 protein complex, whose prior known function is in autophagosome formation, is required for IFN gamma-mediated host defense against murine norovirus (MNV) infection. Importantly, the direct antiviral activity of IFN gamma against MNV in macrophages required Atg5-Atg12, Atg7, and Atg16L1, but not induction of autophagy, the degradative activity of lysosomal proteases, fusion of autophagosomes and lysosomes, or the Atg8-processing protein Atg4B. IFN gamma, via Atg5-Atg12/Atg16L1, inhibited formation of the membranous cytoplasmic MNV replication complex, where Atg16L1 localized. Thus, the Atg5-Atg12/Atg16L1 complex performs a pivotal, nondegradative role in IFN gamma-mediated antiviral defense, establishing that multicellular organisms have evolved to use portions of the autophagy pathway machinery in a cassette-like fashion for host defense. C1 [Hwang, Seungmin; Maloney, Nicole S.; Bruinsma, Monique W.; Duan, Erning; Zhang, Lei; Dani, Adish; Thackray, Larissa B.; Virgin, Herbert W.] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA. [Shrestha, Bimmi; Diamond, Michael S.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. [Goel, Gautam; Xavier, Ramnik J.] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Gastrointestinal Unit, Boston, MA 02114 USA. [Goel, Gautam; Xavier, Ramnik J.] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Study Inflammatory Bowel Dis, Boston, MA 02114 USA. [Sosnovtsev, Stanislav V.; Green, Kim Y.] NIAID, Infect Dis Lab, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Lopez-Otin, Carlos] Univ Oviedo, Inst Univ Oncol, Fac Med, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain. RP Thackray, LB (reprint author), Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA. EM thackray@wustl.edu; virgin@wustl.edu RI zhou, zhenqing/H-4580-2014; Duan, Erning /H-4608-2014; Lopez-Otin, Carlos/C-6657-2013; OI Lopez-Otin, Carlos/0000-0001-6964-1904; Hwang, Seungmin/0000-0003-0846-5462 FU Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Diseases [U54 AI065982]; National Institutes of Health [AI054483, CA096511, AI084887]; Rheumatic Diseases Core Center [NIH P30 AR48335]; NCI Cancer Center [P30 CA91842]; ICTS/CTSA [UL1RR024992]; Ministry of Science and Innovation-Spain; Fundacion M. Botin FX This work was supported by the Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (U54 AI065982), National Institutes of Health grants AI054483, CA096511, and AI084887, and the Rheumatic Diseases Core Center (NIH P30 AR48335). We thank the Genome Technology Access Center at Washington University School of Medicine for help with genomic analysis (supported by NCI Cancer Center Support Grant number P30 CA91842 and ICTS/CTSA grant number UL1RR024992). Washington University and H.W.V. receive income based on licenses for MNV technology. C.L.O. was supported by grants from Ministry of Science and Innovation-Spain, FP7 (Microenvimet), and Fundacion M. Botin. We would like to thank Masaaki Komatsu (Tokyo Metropolitan Institute of Medical Science, Japan) for Atg7F/F mouse, Noboru Mizushima (Tokyo Medical and Dental University, Japan) for Atg5F/F mouse, Tamotsu Yoshimori (Osaka University, Japan) for Atg4B/C74A and Rab7A/T22N constructs. We thank Virgin lab members for their comments on the manuscript and D. Kreamalmeyer and M. White for managing mouse colonies. NR 46 TC 77 Z9 78 U1 3 U2 17 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1931-3128 J9 CELL HOST MICROBE JI Cell Host Microbe PD APR 19 PY 2012 VL 11 IS 4 BP 397 EP 409 DI 10.1016/j.chom.2012.03.002 PG 13 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA 935BH UT WOS:000303491500010 PM 22520467 ER PT J AU Abolfath, RM Biswas, PK Rajnarayanam, R Brabec, T Kodym, R Papiez, L AF Abolfath, Ramin M. Biswas, P. K. Rajnarayanam, R. Brabec, Thomas Kodym, Reinhard Papiez, Lech TI Multiscale QM/MM Molecular Dynamics Study on the First Steps of Guanine Damage by Free Hydroxyl Radicals in Solution SO JOURNAL OF PHYSICAL CHEMISTRY A LA English DT Article ID NUCLEOTIDE EXCISION-REPAIR; MONTE-CARLO-SIMULATION; DNA-BASE DAMAGE; HYDROGEN ABSTRACTION; STRAND SCISSION; FORCE-FIELD; DENSITY; BACKBONE; GROMACS; SITES AB Understanding the damage of DNA bases from hydrogen abstraction by free OH radicals is of particular importance to understanding the indirect effect of ionizing radiation. Previous studies address the problem with truncated DNA bases as ab initio :,quantum simulations required to study Such electronic-spin-dependent processes are computationally expensive. Here, for the first time, we employ a multiscale and hybrid quantum mechanical-molecular mechanical simulation to study the interaction of OH radicals with a guanine-deoxyribose-phosphate DNA molecular unit in the presence of water, where all of the water molecules and the deoxyribose-phosphate fragment are treated with the simplistic classical molecular mechanical scheme. Our result illustrates that the presence of water strongly alters the hydrogen-abstraction reaction as the hydrogen bonding of OH radicals with water restricts the relative orientation of the OH radicals with respect to the DNA base (here, guanine). This results in an angular anisotropy in the chemical pathway and a lower efficiency in the hydrogen-abstraction mechanisms than previously anticipated for identical systems in vacuum. The method can easily be extended to single- and double-stranded DNA without any appreciable computational cost as these molecular units can be treated in the classical subsystem, as has been demonstrated here. C1 [Abolfath, Ramin M.] Univ Texas Dallas, Sch Nat Sci & Math, Richardson, TX 75080 USA. [Abolfath, Ramin M.; Brabec, Thomas] Univ Ottawa, Dept Phys, Ottawa, ON K1N 6N5, Canada. [Biswas, P. K.] Tougaloo Coll, Dept Phys, Tougaloo, MS 39174 USA. [Biswas, P. K.] NHLBI, Lab Computat Biol, NIH, Rockville, MD 20894 USA. [Rajnarayanam, R.] SUNY Buffalo, Dept Pharmacol, Buffalo, NY 14260 USA. [Kodym, Reinhard] Univ Texas SW Med Ctr Dallas, Dept Radiat Oncol, Dallas, TX 75390 USA. [Papiez, Lech] Indiana Univ, Cyclotron Facil, Bloomington, IN 47408 USA. RP Abolfath, RM (reprint author), Yale Univ, Sch Med, Dept Therapeut Radiol, 333 Cedar St, New Haven, CT 06520 USA. EM ramin.abolfath@utdallas.edu; pbiswas@tougaloo.edu FU MS-INBRE/NIH [P20RR016476]; RIMI/NIH [P20MD002725] FX We gratefully acknowledge helpful discussion and inspiring ideas with Dr. David Chen and Dr. Michael Story. P.K.B. acknowledges financial support from MS-INBRE/NIH Grant #P20RR016476 and RIMI/NIH Grant #P20MD002725. NR 42 TC 22 Z9 22 U1 5 U2 49 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1089-5639 J9 J PHYS CHEM A JI J. Phys. Chem. A PD APR 19 PY 2012 VL 116 IS 15 BP 3940 EP 3945 DI 10.1021/jp300258n PG 6 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA 927QI UT WOS:000302924600018 PM 22397677 ER PT J AU Talan, MI Ahmet, I Lakatta, EG AF Talan, Mark I. Ahmet, Ismayil Lakatta, Edward G. TI Did Clinical Trials in Which Erythropoietin Failed to Reduce Acute Myocardial Infarct Size Miss a Narrow Therapeutic Window? SO PLOS ONE LA English DT Article ID RECOMBINANT-HUMAN-ERYTHROPOIETIN; CONTROLLED PILOT TRIAL; CARDIOPROTECTION; REPERFUSION; RATIONALE; REVEAL; DESIGN AB Background: To test a hypothesis that in negative clinical trials of erythropoietin in patients with acute myocardial infarction (MI) the erythropoietin (rhEPO) could be administered outside narrow therapeutic window. Despite overwhelming evidence of cardioprotective properties of rhEPO in animal studies, the outcomes of recently concluded phase II clinical trials have failed to demonstrate the efficacy of rhEPO in patients with acute MI. However, the time between symptoms onset and rhEPO administration in negative clinical trials was much longer that in successful animal experiments. Methodology/Principal Findings: MI was induced in rats either by a permanent ligation of a descending coronary artery or by a 2-hr occlusion followed by a reperfusion. rhEPO, 3000 IU/kg, was administered intraperitoneally at the time of reperfusion, 4 hrs after beginning of reperfusion, or 6 hrs after permanent occlusion. MI size was measured histologically 24 hrs after coronary occlusion. The area of myocardium at risk was similar among groups. The MI size in untreated rats averaged similar to 42% of area at risk, or similar to 24% of left ventricle, and was reduced by more than 50% (p<0.001) in rats treated with rhEPO at the time of reperfusion. The MI size was not affected by treatment administered 4 hrs after reperfusion or 6 hrs after permanent coronary occlusion. Therefore, our study in a rat experimental model of MI demonstrates that rhEPO administered within 2 hrs of a coronary occlusion effectively reduces MI size, but when rhEPO was administered following a delay similar to that encountered in clinical trials, it had no effect on MI size. Conclusions/Significance: The clinical trials that failed to demonstrate rhEPO efficacy in patients with MI may have missed a narrow therapeutic window defined in animal experiments. C1 [Talan, Mark I.; Ahmet, Ismayil; Lakatta, Edward G.] NIA, Cardiovasc Sci Lab, Intramural Res Program, Natl Inst Hlth, Baltimore, MD 21224 USA. RP Talan, MI (reprint author), NIA, Cardiovasc Sci Lab, Intramural Res Program, Natl Inst Hlth, Baltimore, MD 21224 USA. EM talanm@grc.nia.nih.gov NR 25 TC 10 Z9 10 U1 1 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2012 VL 7 IS 4 AR e34819 DI 10.1371/journal.pone.0034819 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959XD UT WOS:000305350600032 PM 22529941 ER PT J AU Wu, WW Wang, GH Insel, PA Hsiao, CT Zou, SG Martin, B Maudsley, S Shen, RF AF Wu, Wells W. Wang, Guanghui Insel, Paul A. Hsiao, Cheng-Te Zou, Sige Martin, Bronwen Maudsley, Stuart Shen, Rong-Fong TI Discovery- and target-based protein quantification using iTRAQ and pulsed Q collision induced dissociation (PQD) SO JOURNAL OF PROTEOMICS LA English DT Article DE Pulsed Q collision-induced dissociation (PQD); Linear ion trap; Triple quadrupole (QqQ); Higher energy collisional dissociation (HCD); iTRAQ (Isobaric Tag for Relative and Absolute Quantification) ID TANDEM MASS-SPECTROMETRY; QUADRUPOLE ION-TRAP; IDENTIFICATION; QUANTITATION; ACQUISITION; PROTEOMICS; SCAN AB Pulsed Q collision-induced dissociation (PQD) was developed in part to facilitate detection of low-mass reporter ions using labeling reagents (e.g. iTRAQ) on LTQ platforms. It has generally been recognized that the scan speed and sensitivity of an LTQ are superior than those of an Orbitrap using the higher-energy collisional dissociation (HCD). However, the use of PQD in quantitative proteomics is limited, primarily due to the meager reproducibility of reporter ion ratios. Optimizations of PQD for iTRAQ quantification using LTQ have been reported, but a universally applicable strategy for quantifying the less abundant proteins has not been fully established. Adjustments of the AGC target, mu scan, or scan speed offer only incremental improvements in reproducibility. From our experience, however, satisfactory coefficients of variation (CVs) of reporter ion ratios were difficult to achieve using the discovery-based approach. As an alternative, we implemented a target-based approach that obviates data dependency to allow repetitive data acquisitions across chromatographic peaks. Such a strategy generates enough data points for more reliable quantification. Using cAMP treatment in S49 cell lysates and this target-based approach, we were able to validate differentially expressed proteins, which were initially identified as potential candidates using the discovery-based PQD. The target-based strategy also yielded results comparable to those obtained from HCD in an Orbitrap. Our findings should aid LTQ users who desire to explore iTRAQ quantitative proteomics but have limited access to the more costly Orbitrap or other instruments. Published by Elsevier B.V. C1 [Maudsley, Stuart] NIA, Receptor Pharmacol Unit, NIH, Baltimore, MD 21224 USA. [Wu, Wells W.; Martin, Bronwen] NIA, Metab Unit, NIH, Baltimore, MD 21224 USA. [Wang, Guanghui] NHLBI, Prote Core Facil, NIH, Bethesda, MD 20892 USA. [Insel, Paul A.] Univ Calif San Diego, Dept Pharmacol & Med, La Jolla, CA 92093 USA. [Hsiao, Cheng-Te; Zou, Sige] NIA, Funct Genom Unit, NIH, Baltimore, MD 21224 USA. [Shen, Rong-Fong] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA. RP Maudsley, S (reprint author), NIA, Receptor Pharmacol Unit, NIH, 251 Bayview Blvd, Baltimore, MD 21224 USA. EM maudsleyst@grc.nia.nih.gov; Rongfong.Shen@fda.hhs.gov FU National Institute on Aging; National Heart, Lung, and Blood Institute, National Institutes of Health (NIH) FX This research was supported by the Intramural Research Programs of National Institute on Aging and National Heart, Lung, and Blood Institute, National Institutes of Health (NIH). The authors would like to acknowledge the following for technical advice: Dr. Howard Jaffe, NINDS, NIH; Drs. Jae C. Schwartz, Mike W. Senko, and John E.P. Syka of ThermoFisher Scientific Inc. NR 23 TC 11 Z9 11 U1 0 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 J9 J PROTEOMICS JI J. Proteomics PD APR 18 PY 2012 VL 75 IS 8 BP 2480 EP 2487 DI 10.1016/j.jprot.2012.02.027 PG 8 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 941PJ UT WOS:000303974800018 PM 22397766 ER PT J AU Beisel, CL Updegrove, TB Janson, B Storz, G AF Beisel, Chase L. Updegrove, Taylor B. Janson, Ben J. Storz, Gisela TI Multiple factors dictate target selection by Hfq-binding small RNAs SO EMBO JOURNAL LA English DT Article DE base-pairing; Escherichia coli; Spot 42; target prediction ID ESCHERICHIA-COLI; SOLUBLE-RNA; POSTTRANSCRIPTIONAL CONTROL; TRANSLATIONAL INITIATION; CATABOLITE REPRESSION; REGULATORY RNAS; GENE-EXPRESSION; SPOT 42; PREDICTION; GCVB AB Hfq-binding small RNAs (sRNAs) in bacteria modulate the stability and translational efficiency of target mRNAs through limited base-pairing interactions. While these sRNAs are known to regulate numerous mRNAs as part of stress responses, what distinguishes targets and non-targets among the mRNAs predicted to base pair with Hfq-binding sRNAs is poorly understood. Using the Hfq-binding sRNA Spot 42 of Escherichia coli as a model, we found that predictions using only the three unstructured regions of Spot 42 substantially improved the identification of previously known and novel Spot 42 targets. Furthermore, increasing the extent of base-pairing in single or multiple base-pairing regions improved the strength of regulation, but only for the unstructured regions of Spot 42. We also found that non-targets predicted to base pair with Spot 42 lacked an Hfq-binding site, folded into a secondary structure that occluded the Spot 42 targeting site, or had overlapping Hfq-binding and targeting sites. By modifying these features, we could impart Spot 42 regulation on non-target mRNAs. Our results thus provide valuable insights into the requirements for target selection by sRNAs. The EMBO Journal (2012) 31, 1961-1974. doi:10.1038/emboj.2012.52; Published online 2 March 2012 Subject Categories: RNA C1 [Beisel, Chase L.; Updegrove, Taylor B.; Janson, Ben J.; Storz, Gisela] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cell Biol & Metab Program, Bethesda, MD 20892 USA. RP Beisel, CL (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cell Biol & Metab Program, 18 Lib Dr,MSC 5430, Bethesda, MD 20892 USA. EM cbeisel@ncsu.edu; storz@helix.nih.gov RI Beisel, Chase/D-4823-2015; OI Beisel, Chase/0000-0003-0650-9943; Storz, Gisela/0000-0001-6698-1241 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development FX We thank B Tjaden, S Gottesman, and members of the Storz laboratory for helpful discussions and critical reading of the manuscript. We are grateful to C Sharma for technical assistance, to A Zhang for providing the hfq mutant allele, purified Hfq and alpha-Hfq serum, and to MK Thomason and A Zhang for sharing unpublished data. Work carried out in the laboratory of GS was supported by the Intramural Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development. CLB is a Gordon and Betty Moore Foundation Fellow of the Life Sciences Research Foundation. NR 51 TC 49 Z9 49 U1 0 U2 9 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0261-4189 J9 EMBO J JI Embo J. PD APR 18 PY 2012 VL 31 IS 8 BP 1961 EP 1974 DI 10.1038/emboj.2012.52 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 930AV UT WOS:000303108600011 PM 22388518 ER PT J AU Patten, ML Murphy, AP AF Patten, Matthew L. Murphy, Aidan P. TI Relative Disparity Processing in the Dorsal Visual Pathway SO JOURNAL OF NEUROSCIENCE LA English DT Editorial Material ID STEREOSCOPIC DEPTH-PERCEPTION; NEURONS; AREA; CORTEX; REPRESENTATION; RESPONSES; COARSE; MOTION; SIGNAL; MT C1 [Patten, Matthew L.; Murphy, Aidan P.] Univ Birmingham, Sch Psychol, Birmingham B15 2TT, W Midlands, England. [Murphy, Aidan P.] NIMH, Sect Cognit Neurophysiol & Imaging, Neuropsychol Lab, Bethesda, MD 20892 USA. RP Patten, ML (reprint author), Univ Birmingham, Sch Psychol, Birmingham B15 2TT, W Midlands, England. EM m.l.patten@bham.ac.uk FU Wellcome Trust [091467, WT091467MA] NR 12 TC 0 Z9 0 U1 0 U2 6 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD APR 18 PY 2012 VL 32 IS 16 BP 5353 EP 5355 DI 10.1523/JNEUROSCI.0588-12.2012 PG 3 WC Neurosciences SC Neurosciences & Neurology GA 929FV UT WOS:000303047600001 PM 22514299 ER PT J AU Roesch, MR Bryden, DW Cerri, DH Haney, ZR Schoenbaum, G AF Roesch, Matthew R. Bryden, Daniel W. Cerri, Domenic H. Haney, Zachary R. Schoenbaum, Geoffrey TI Willingness to Wait and Altered Encoding of Time-Discounted Reward in the Orbitofrontal Cortex with Normal Aging SO JOURNAL OF NEUROSCIENCE LA English DT Article ID NUCLEUS-ACCUMBENS CORE; ORBITAL PREFRONTAL CORTEX; WORKING-MEMORY IMPAIRMENT; IMPULSIVE CHOICE; DECISION-MAKING; BASOLATERAL AMYGDALA; AGED RATS; PROBABILISTIC REINFORCEMENT; VENTRAL STRIATUM; DIFFERENT FORMS AB Normal aging has been associated with cognitive changes, including shifts in responding for time-discounted rewards. The orbitofrontal cortex, an area previously associated with aging-related cognitive changes, is critical for normal discounting. Previously we have shown in a choice task that rats prefer immediate over delayed reward and that neural representations of delayed reward in orbitofrontal cortex were attenuated, whereas immediate reward elicited strong responses. Changes in choice performance were correlated with changes in firing rate in orbitofrontal neurons, suggesting that these reward representations were critical to the rats' ability to wait for reward. Here we asked whether age-dependent changes in discounting behavior were related to changes in the representation of delayed reward in the orbitofrontal cortex. Young (3-6 months) and aged (22-26 months) rats were trained on the same discounting paradigm used previously. We found that aged rats showed less sensitivity to increasing delay preceding reward delivery, shifting behavior away from the delayed reward more slowly than younger rats. This sensitivity was specific to delay, since choice performance did not differ between the two groups when delay was held constant and reward size varied. Aged rats exhibited a corresponding increase in the prevalence of neurons that fired more strongly for delayed reward. Again this change was specific to delay; there was no change in encoding of different-sized rewards. These results suggest that natural aging results in altered representations of reward in orbitofrontal cortex. These changes may relate to the increased ability to delay gratification and reduced impulsivity associated with aging. C1 [Schoenbaum, Geoffrey] NIDA, Intramural Res Program, Baltimore, MD 21224 USA. [Roesch, Matthew R.; Bryden, Daniel W.] Univ Maryland, Dept Psychol, College Pk, MD 20742 USA. [Roesch, Matthew R.; Bryden, Daniel W.] Univ Maryland, Program Neurosci & Cognit Sci, College Pk, MD 20742 USA. [Cerri, Domenic H.; Haney, Zachary R.] Univ Maryland, Sch Med, Dept Anat & Neurobiol, Baltimore, MD 21201 USA. RP Schoenbaum, G (reprint author), NIDA, Intramural Res Program, Baltimore, MD 21224 USA. EM mroesch@umd.edu; schoenbg@schoenbaumlab.org FU NIA; NIDA FX This work was supported by grants from NIA to G.S. and NIDA to M.R.R. This article was prepared in part while G.S. was employed at University of Maryland School of Medicine, Baltimore. The opinions expressed in this article are the author's own and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. NR 54 TC 15 Z9 15 U1 0 U2 8 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD APR 18 PY 2012 VL 32 IS 16 BP 5525 EP 5533 DI 10.1523/JNEUROSCI.0586-12.2012 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 929FV UT WOS:000303047600016 PM 22514314 ER PT J AU Basch, E Aronson, N Berg, A Flum, D Gabriel, S Goodman, SN Helfand, M Ioannidis, JPA Lauer, M Meltzer, D Mittman, B Newhouse, R Normand, SL Schneeweiss, S Slutsky, J Tinetti, M Yancy, C AF Basch, Ethan Aronson, Naomi Berg, Alfred Flum, David Gabriel, Sherine Goodman, Steven N. Helfand, Mark Ioannidis, John P. A. Lauer, Michael Meltzer, David Mittman, Brian Newhouse, Robin Normand, Sharon-Lise Schneeweiss, Sebastian Slutsky, Jean Tinetti, Mary Yancy, Clyde CA Methodology Comm Patient-Centered TI Methodological Standards and Patient-Centeredness in Comparative Effectiveness Research The PCORI Perspective SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID IMPLEMENTATION-RESEARCH; RANDOMIZED-TRIALS; PROPENSITY SCORE; CLINICAL-TRIALS; QUALITY IMPROVEMENT; PUBLICATION BIAS; MEDICATIONS; EXPERIENCE; BENEFITS; LESSONS AB Rigorous methodological standards help to ensure that medical research produces information that is valid and generalizable, and are essential in patient-centered outcomes research (PCOR). Patient-centeredness refers to the extent to which the preferences, decision-making needs, and characteristics of patients are addressed, and is the key characteristic differentiating PCOR from comparative effectiveness research. The Patient Protection and Affordable Care Act signed into law in 2010 created the Patient-Centered Outcomes Research Institute (PCORI), which includes an independent, federally appointed Methodology Committee. The Methodology Committee is charged to develop methodological standards for PCOR. The 4 general areas identified by the committee in which standards will be developed are (1) prioritizing research questions, (2) using appropriate study designs and analyses, (3) incorporating patient perspectives throughout the research continuum, and (4) fostering efficient dissemination and implementation of results. A Congressionally mandated PCORI methodology report (to be issued in its first iteration in May 2012) will begin to provide standards in each of these areas, and will inform future PCORI funding announcements and review criteria. The work of the Methodology Committee is intended to enable generation of information that is relevant and trustworthy for patients, and to enable decisions that improve patient-centered outcomes. JAMA. 2012;307(15):1636-1640 www.jama.com C1 [Basch, Ethan] Mem Sloan Kettering Canc Ctr, Hlth Outcomes Res Grp, New York, NY 10463 USA. [Aronson, Naomi] Blue Cross Blue Shield Assoc, Chicago, IL USA. [Berg, Alfred; Flum, David] Univ Washington, Seattle, WA 98195 USA. [Gabriel, Sherine] Mayo Clin, Rochester, MN USA. [Goodman, Steven N.; Ioannidis, John P. A.] Stanford Univ, Stanford, CA 94305 USA. [Helfand, Mark] Portland VA Med Ctr, Portland, OR USA. [Lauer, Michael] NHLBI, Bethesda, MD 20892 USA. [Meltzer, David] Univ Chicago, Chicago, IL 60637 USA. [Mittman, Brian] Dept Vet Affairs, Sepulveda, CA USA. [Newhouse, Robin] Univ Maryland, Baltimore, MD 21201 USA. [Normand, Sharon-Lise; Schneeweiss, Sebastian] Harvard Univ, Sch Med, Boston, MA USA. [Normand, Sharon-Lise] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. [Slutsky, Jean] Agcy Healthcare Res & Qual, Rockville, MD USA. [Tinetti, Mary] Yale Univ, New Haven, CT USA. [Yancy, Clyde] Northwestern Univ, Chicago, IL 60611 USA. RP Basch, E (reprint author), Mem Sloan Kettering Canc Ctr, Hlth Outcomes Res Grp, 307 63rd St, New York, NY 10463 USA. EM ebasch@mskcc.org RI Schneeweiss, Sebastian/C-2125-2013; Meltzer, David/C-2926-2009 OI Meltzer, David/0000-0003-2790-7393 FU PCORI of the Methodology Committee FX The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. All of the authors reported receiving funding from PCORI as members of the Methodology Committee, including compensation for travel and lodging for PCORI-related meetings as well as time spent providing service to the PCORI. NR 45 TC 96 Z9 96 U1 3 U2 26 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 18 PY 2012 VL 307 IS 15 BP 1636 EP 1640 DI 10.1001/jama.2012.466 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA 927GU UT WOS:000302896100026 ER PT J AU Kannan, S Dai, H Navath, RS Balakrishnan, B Jyoti, A Janisse, J Romero, R Kannan, RM AF Kannan, Sujatha Dai, Hui Navath, Raghavendra S. Balakrishnan, Bindu Jyoti, Amar Janisse, James Romero, Roberto Kannan, Rangaramanujam M. TI Dendrimer-Based Postnatal Therapy for Neuroinflammation and Cerebral Palsy in a Rabbit Model SO SCIENCE TRANSLATIONAL MEDICINE LA English DT Article ID BLOOD-BRAIN-BARRIER; N-ACETYLCYSTEINE; MICROGLIAL ACTIVATION; ALZHEIMERS-DISEASE; PERINATAL ASPHYXIA; GLUTATHIONE LEVELS; MOTOR DEFICITS; DRUG-DELIVERY; WHITE-MATTER; CELLS AB Cerebral palsy (CP) is a chronic childhood disorder with no effective cure. Neuroinflammation, caused by activated microglia and astrocytes, plays a key role in the pathogenesis of CP and disorders such as Alzheimer's disease and multiple sclerosis. Targeting neuroinflammation can be a potent therapeutic strategy. However, delivering drugs across the blood-brain barrier to the target cells for treating diffuse brain injury is a major challenge. We show that systemically administered polyamidoamine dendrimers localize in activated microglia and astrocytes in the brain of newborn rabbits with CP, but not healthy controls. We further demonstrate that dendrimer-based N-acetyl-L-cysteine (NAC) therapy for brain injury suppresses neuroinflammation and leads to a marked improvement in motor function in the CP kits. The well-known and safe clinical profile for NAC, when combined with dendrimer-based targeting, provides opportunities for clinical translation in the treatment of neuroinflammatory disorders in humans. The effectiveness of the dendrimer-NAC treatment, administered in the postnatal period for a prenatal insult, suggests a window of opportunity for treatment of CP in humans after birth. C1 [Kannan, Sujatha; Dai, Hui; Navath, Raghavendra S.; Balakrishnan, Bindu; Jyoti, Amar; Romero, Roberto; Kannan, Rangaramanujam M.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Perinatol Res Branch, NIH, Detroit, MI 48201 USA. [Kannan, Sujatha; Dai, Hui; Balakrishnan, Bindu; Jyoti, Amar] Wayne State Univ, Detroit Med Ctr, Childrens Hosp Michigan, Dept Pediat, Detroit, MI 48201 USA. [Navath, Raghavendra S.; Kannan, Rangaramanujam M.] Wayne State Univ, Dept Chem Engn & Mat Sci, Detroit, MI 48201 USA. [Janisse, James] Wayne State Univ, Dept Family Med & Publ Hlth Sci, Detroit, MI 48201 USA. RP Kannan, S (reprint author), Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21287 USA. EM skannan3@jhmi.edu; romeror@mail.nih.gov; krangar1@jhmi.edu FU Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), NIH; NICHD [5K08HD050652] FX Supported in part by the Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), NIH, and by NICHD 5K08HD050652 (S.K.). NR 52 TC 57 Z9 58 U1 2 U2 28 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 1946-6234 J9 SCI TRANSL MED JI Sci. Transl. Med. PD APR 18 PY 2012 VL 4 IS 130 AR 130ra46 DI 10.1126/scitranslmed.3003162 PG 11 WC Cell Biology; Medicine, Research & Experimental SC Cell Biology; Research & Experimental Medicine GA 929FE UT WOS:000303045900003 PM 22517883 ER PT J AU Boratyn, GM Schaffer, AA Agarwala, R Altschul, SF Lipman, DJ Madden, TL AF Boratyn, Grzegorz M. Schaeffer, Alejandro A. Agarwala, Richa Altschul, Stephen F. Lipman, David J. Madden, Thomas L. TI Domain enhanced lookup time accelerated BLAST SO BIOLOGY DIRECT LA English DT Article ID DISTANTLY RELATED PROTEINS; DNA-BINDING SITES; PSI-BLAST; STRUCTURE ALIGNMENTS; REGULATORY PROTEINS; SEQUENCE ALIGNMENT; DATABASE SEARCHES; SCORE MATRICES; ACID SEQUENCES; INFORMATION AB Background: BLAST is a commonly-used software package for comparing a query sequence to a database of known sequences; in this study, we focus on protein sequences. Position-specific-iterated BLAST (PSI-BLAST) iteratively searches a protein sequence database, using the matches in round i to construct a position-specific score matrix (PSSM) for searching the database in round i + 1. Biegert and Soding developed Context-sensitive BLAST (CS-BLAST), which combines information from searching the sequence database with information derived from a library of short protein profiles to achieve better homology detection than PSI-BLAST, which builds its PSSMs from scratch. Results: We describe a new method, called domain enhanced lookup time accelerated BLAST (DELTA-BLAST), which searches a database of pre-constructed PSSMs before searching a protein-sequence database, to yield better homology detection. For its PSSMs, DELTA-BLAST employs a subset of NCBI's Conserved Domain Database (CDD). On a test set derived from ASTRAL, with one round of searching, DELTA-BLAST achieves a ROC5000 of 0.270 vs. 0.116 for CS-BLAST. The performance advantage diminishes in iterated searches, but DELTA-BLAST continues to achieve better ROC scores than CS-BLAST. Conclusions: DELTA-BLAST is a useful program for the detection of remote protein homologs. It is available under the "Protein BLAST" link at http://blast.ncbi.nlm.nih.gov. C1 [Boratyn, Grzegorz M.; Schaeffer, Alejandro A.; Agarwala, Richa; Altschul, Stephen F.; Lipman, David J.; Madden, Thomas L.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. RP Boratyn, GM (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bldg 38A,8600 Rockville Pike, Bethesda, MD 20894 USA. EM boratyng@ncbi.nlm.nih.gov FU Intramural Research Program of the NIH, National Library of Medicine FX This research was supported by the Intramural Research Program of the NIH, National Library of Medicine. NR 43 TC 155 Z9 156 U1 2 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1745-6150 J9 BIOL DIRECT JI Biol. Direct PD APR 17 PY 2012 VL 7 AR 12 DI 10.1186/1745-6150-7-12 PG 15 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 003GJ UT WOS:000308596200001 PM 22510480 ER PT J AU Du, GW Lewis, MM Shaffer, ML Chen, HL Yang, QX Mailman, RB Huang, XM AF Du, Guangwei Lewis, Mechelle M. Shaffer, Michele L. Chen, Honglei Yang, Qing X. Mailman, Richard B. Huang, Xuemei TI Serum Cholesterol and Nigrostriatal R2*Values in Parkinson's Disease SO PLOS ONE LA English DT Article ID BRAIN IRON ACCUMULATION; SUBSTANTIA-NIGRA; BASAL GANGLIA; NEURODEGENERATIVE DISEASE; ALZHEIMERS-DISEASE; ALPHA-SYNUCLEIN; RISK-FACTORS; IN-VITRO; TRANSFERRIN; DISORDERS AB Background: The occurrence of Parkinson's disease (PD) is known to be associated both with increased nigrostriatal iron content and with low serum cholesterol and PD, but there has been no study to determine a potential relationship between these two factors. Methods: High-resolution MRI (T1-, T2, and multiple echo T2*-weighted imaging) and fasting lipid levels were obtained from 40 patients with PD and 29 healthy controls. Iron content was estimated from mean R2* values (R2* = 1/T2*) calculated for each nigrostriatal structure including substantia nigra, caudate, putamen, and globus pallidus. This was correlated with serum cholesterol levels after controlling for age, gender, and statin use. Results: In patients with PD, higher serum cholesterol levels were associated with lower iron content in the substantia nigra (R = -0.43, p = 0.011 for total-cholesterol, R = -0.31, p = 0.080 for low-density lipoprotein) and globus pallidus (R = -0.38, p = 0.028 for total-cholesterol, R = -0.27, p = 0.127 for low-density lipoprotein), but only a trend toward significant association of higher total-cholesterol with lower iron content in the striatum (R = -0.34, p = 0.052 for caudate; R = -0.32, p = 0.061 for putamen). After adjusting for clinical measures, the cholesterol-iron relationships held or became even stronger in the substantia nigra and globus pallidus, but weaker in the caudate and putamen. There was no significant association between serum cholesterol levels and nigrostriatal iron content for controls. Conclusions: The data show that higher serum total-cholesterol concentration is associated with lower iron content in substantia nigra and globus pallidus in Parkinson's disease patients. Further studies should investigate whether this is mechanistic or epiphenomenological relationship. C1 [Du, Guangwei; Lewis, Mechelle M.; Mailman, Richard B.; Huang, Xuemei] Penn State Univ, Milton S Hershey Med Ctr, Dept Neurol, Hershey, PA 17033 USA. [Lewis, Mechelle M.; Mailman, Richard B.; Huang, Xuemei] Penn State Univ, Milton S Hershey Med Ctr, Dept Pharmacol, Hershey, PA 17033 USA. [Yang, Qing X.; Huang, Xuemei] Penn State Univ, Milton S Hershey Med Ctr, Dept Radiol, Hershey, PA 17033 USA. [Yang, Qing X.; Huang, Xuemei] Penn State Univ, Milton S Hershey Med Ctr, Dept Neurosurg, Hershey, PA 17033 USA. [Huang, Xuemei] Penn State Univ, Milton S Hershey Med Ctr, Dept Kinesiol, Hershey, PA 17033 USA. [Shaffer, Michele L.] Penn State Univ, Milton S Hershey Med Ctr, Dept Publ Hlth Sci, Hershey, PA 17033 USA. [Chen, Honglei] NIEHS, Epidemiol Branch, Res Triangle Pk, NC 27709 USA. RP Du, GW (reprint author), Penn State Univ, Milton S Hershey Med Ctr, Dept Neurol, Hershey, PA 17033 USA. EM Xuemei@psu.edu OI Mailman, Richard/0000-0003-1353-2738; Chen, Honglei/0000-0003-3446-7779 FU National Institute of Neurological Disorders and Stroke [NS060722]; General Clinical Research Center from National Institutes of Health (NIH) [M01RR10732]; GCRC [C06RR016499]; Pennsylvania Department of Health Tobacco CURE Funds; National Institute of Health; National Institute of Environmental Health Sciences FX This work was supported by National Institute of Neurological Disorders and Stroke (NS060722 to XH), General Clinical Research Center grant from National Institutes of Health (NIH) (M01RR10732) and GCRC Construction Grant (C06RR016499) awarded to the Pennsylvania State University College of Medicine, the Pennsylvania Department of Health Tobacco CURE Funds, and the intramural research program of the National Institute of Health and the National Institute of Environmental Health Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 7 Z9 7 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 17 PY 2012 VL 7 IS 4 AR e35397 DI 10.1371/journal.pone.0035397 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WC UT WOS:000305347400049 PM 22530017 ER PT J AU Kwan, JY Jeong, SY Van Gelderen, P Deng, HX Quezado, MM Danielian, LE Butman, JA Chen, LY Bayat, E Russell, J Siddique, T Duyn, JH Rouault, TA Floeter, MK AF Kwan, Justin Y. Jeong, Suh Young Van Gelderen, Peter Deng, Han-Xiang Quezado, Martha M. Danielian, Laura E. Butman, John A. Chen, Lingye Bayat, Elham Russell, James Siddique, Teepu Duyn, Jeff H. Rouault, Tracey A. Floeter, Mary Kay TI Iron Accumulation in Deep Cortical Layers Accounts for MRI Signal Abnormalities in ALS: Correlating 7 Tesla MRI and Pathology SO PLOS ONE LA English DT Article ID AMYOTROPHIC-LATERAL-SCLEROSIS; HIGH-FIELD MRI; PRECENTRAL GYRUS; CORTICOSPINAL TRACT; MAGNETIC-RESONANCE; PARKINSONS-DISEASE; BRAIN IRON; CELLULAR-DISTRIBUTION; MOTOR CORTEX; WHITE-MATTER AB Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder characterized by cortical and spinal motor neuron dysfunction. Routine magnetic resonance imaging (MRI) studies have previously shown hypointense signal in the motor cortex on T-2-weighted images in some ALS patients, however, the cause of this finding is unknown. To investigate the utility of this MR signal change as a marker of cortical motor neuron degeneration, signal abnormalities on 3T and 7T MR images of the brain were compared, and pathology was obtained in two ALS patients to determine the origin of the motor cortex hypointensity. Nineteen patients with clinically probable or definite ALS by El Escorial criteria and 19 healthy controls underwent 3T MRI. A 7T MRI scan was carried out on five ALS patients who had motor cortex hypointensity on the 3T FLAIR sequence and on three healthy controls. Postmortem 7T MRI of the brain was performed in one ALS patient and histological studies of the brains and spinal cords were obtained post-mortem in two patients. The motor cortex hypointensity on 3T FLAIR images was present in greater frequency in ALS patients. Increased hypointensity correlated with greater severity of upper motor neuron impairment. Analysis of 7T T-2*-weighted gradient echo imaging localized the signal alteration to the deeper layers of the motor cortex in both ALS patients. Pathological studies showed increased iron accumulation in microglial cells in areas corresponding to the location of the signal changes on the 3T and 7T MRI of the motor cortex. These findings indicate that the motor cortex hypointensity on 3T MRI FLAIR images in ALS is due to increased iron accumulation by microglia. C1 [Kwan, Justin Y.; Danielian, Laura E.; Chen, Lingye; Floeter, Mary Kay] NINDS, NIH, Bethesda, MD 20892 USA. [Jeong, Suh Young; Rouault, Tracey A.] NICHHD, Program Mol Med, NIH, Bethesda, MD 20892 USA. [Van Gelderen, Peter; Duyn, Jeff H.] NINDS, Adv MRI Sect, Lab Funct & Mol Imaging, NIH, Bethesda, MD 20892 USA. [Deng, Han-Xiang; Siddique, Teepu] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. [Quezado, Martha M.] NCI, NIH, Bethesda, MD 20892 USA. [Butman, John A.] NIH, Ctr Clin, Bethesda, MD 20892 USA. [Bayat, Elham] George Washington Univ, Dept Neurol, Washington, DC USA. [Russell, James] Univ Maryland, Dept Neurol, Baltimore, MD 21201 USA. RP Kwan, JY (reprint author), NINDS, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA. EM kwanjy@ninds.nih.gov RI Butman, John/J-2780-2013; OI Butman, John/0000-0002-1547-9195; Jeong, Suh Young/0000-0002-6376-7001 FU National Institute of Neurological Disorders and Stroke, National Institutes of Health [1ZIANS002976-12]; National Institute of Aging [P01-AG07232, R37-AG15473, P50-AG08702]; Hereditary Disease Foundation; Iseman Fund; Louis and Rachel Rudin Foundation FX This research was supported by the Intramural Research Program of the National Institute of Neurological Disorders and Stroke, National Institutes of Health (1ZIANS002976-12). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The control tissue was provided by the New York Brain Bank - The Taub Institute, Columbia University (NYBB), which is supported by the National Institute of Aging (P01-AG07232, R37-AG15473, and P50-AG08702), the Hereditary Disease Foundation, the Iseman Fund, and the Louis and Rachel Rudin Foundation. We gratefully acknowledge the assistance of Dr. Tian-Xia Wu with statistics and Dr. Esther Mayron-Holtz for providing the ferritin antibody. The protocol is registered on clinicaltrials.gov as NCT00334516. NR 64 TC 52 Z9 52 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 17 PY 2012 VL 7 IS 4 AR e35241 DI 10.1371/journal.pone.0035241 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WC UT WOS:000305347400037 PM 22529995 ER PT J AU Temiz, NA Donohue, DE Bacolla, A Luke, BT Collins, JR AF Temiz, Nuri A. Donohue, Duncan E. Bacolla, Albino Luke, Brian T. Collins, Jack R. TI The Role of Methylation in the Intrinsic Dynamics of B- and Z-DNA SO PLOS ONE LA English DT Article ID UNIQUE TETRANUCLEOTIDE SEQUENCES; MOLECULAR-DYNAMICS; NUCLEIC-ACIDS; Z TRANSITION; FORCE-FIELD; TO-Z; CONFORMATIONS; OLIGONUCLEOTIDES; SIMULATIONS; FLEXIBILITY AB Methylation of cytosine at the 5-carbon position (5mC) is observed in both prokaryotes and eukaryotes. In humans, DNA methylation at CpG sites plays an important role in gene regulation and has been implicated in development, gene silencing, and cancer. In addition, the CpG dinucleotide is a known hot spot for pathologic mutations genome-wide. CpG tracts may adopt left-handed Z-DNA conformations, which have also been implicated in gene regulation and genomic instability. Methylation facilitates this B-Z transition but the underlying mechanism remains unclear. Herein, four structural models of the dinucleotide d(GC)(5) repeat sequence in B-, methylated B-, Z-, and methylated Z-DNA forms were constructed and an aggregate 100 nanoseconds of molecular dynamics simulations in explicit solvent under physiological conditions was performed for each model. Both unmethylated and methylated B-DNA were found to be more flexible than Z-DNA. However, methylation significantly destabilized the BII, relative to the BI, state through the Gp5mC steps. In addition, methylation decreased the free energy difference between B- and Z-DNA. Comparisons of alpha/gamma backbone torsional angles showed that torsional states changed marginally upon methylation for B-DNA, and Z-DNA. Methylation-induced conformational changes and lower energy differences may contribute to the transition to Z-DNA by methylated, over unmethylated, B-DNA and may be a contributing factor to biological function. C1 [Temiz, Nuri A.; Donohue, Duncan E.; Bacolla, Albino; Luke, Brian T.; Collins, Jack R.] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Informat Syst Program, Adv Biomed Comp Ctr,Sil Res Ctr Excellence, Frederick, MD USA. [Bacolla, Albino] Univ Texas MD Anderson Canc Ctr, Dept Mol Carcinogenesis, Smithville, TX USA. RP Temiz, NA (reprint author), SAIC Frederick Inc, Frederick Natl Lab Canc Res, Informat Syst Program, Adv Biomed Comp Ctr,Sil Res Ctr Excellence, Frederick, MD USA. EM temizna@mail.nih.gov RI Bacolla, Albino/N-3877-2013 OI Bacolla, Albino/0000-0003-0206-8423 FU Center for Biomedical Informatics and Information Technology (CBIIT)/Cancer Biomedical Informatics Grid (caBIG) ISRCE yellow task [09-260]; National Cancer Institute/National Institutes of Health [HHSN261200800001E] FX This work was supported by the Center for Biomedical Informatics and Information Technology (CBIIT)/Cancer Biomedical Informatics Grid (caBIG) ISRCE yellow task #09-260 to the Frederick National Laboratory for Cancer Research and National Cancer Institute/National Institutes of Health contract HHSN261200800001E (to AB). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U. S. Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 18 Z9 19 U1 5 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 17 PY 2012 VL 7 IS 4 AR e35558 DI 10.1371/journal.pone.0035558 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WC UT WOS:000305347400062 PM 22530050 ER PT J AU Schuleri, KH Centola, M Evers, KS Zviman, A Evers, R Lima, JAC Lardo, AC AF Schuleri, Karl H. Centola, Marco Evers, Kristine S. Zviman, Adam Evers, Robert Lima, Joao A. C. Lardo, Albert C. TI Cardiovascular magnetic resonance characterization of peri-infarct zone remodeling following myocardial infarction SO JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE LA English DT Article DE Cardiovascular magnetic resonance imaging; Myocardial infarction; Late gadolinium enhancement; Periinfarct zone; Myocardial strain ID LEFT-VENTRICULAR DYSFUNCTION; IMPLANTABLE CARDIOVERTER-DEFIBRILLATOR; ISCHEMIC CARDIOMYOPATHY; TISSUE HETEROGENEITY; HEART-FAILURE; BORDER ZONE; STRAIN; RESYNCHRONIZATION; DYSSYNCHRONY; TACHYCARDIA AB Background: Clinical studies implementing late gadolinium-enhanced (LGE) cardiovascular magnetic resonance (CMR) studies suggest that the peri-infarct zone (PIZ) contains a mixture of viable and non-viable myocytes, and is associated with greater susceptibility to ventricular tachycardia induction and adverse cardiac outcomes. However, CMR data assessing the temporal formation and functional remodeling characteristics of this complex region are limited. We intended to characterize early temporal changes in scar morphology and regional function in the PIZ. Methods and results: CMR studies were performed at six time points up to 90 days after induction of myocardial infarction (MI) in eight minipigs with reperfused, anterior-septal infarcts. Custom signal density threshold algorithms, based on the remote myocardium, were applied to define the infarct core and PIZ region for each time point. After the initial post-MI edema subsided, the PIZ decreased by 54% from day 10 to day 90 (p = 0.04). The size of infarct scar expanded by 14% and thinned by 56% from day 3 to 12 weeks (p = 0.004 and p < 0.001, respectively). LVEDV increased from 34.7 +/- 2.2 ml to 47.8 +/- 3.0 ml (day3 and week12, respectively; p < 0.001). At 30 days post-MI, regional circumferential strain was increased between the infarct scar and the PIZ (-2.1 +/- 0.6 and -6.8 +/- 0.9, respectively;* p < 0.05). Conclusions: The PIZ is dynamic and decreases in mass following reperfused MI. Tensile forces in the PIZ undergo changes following MI. Remodeling characteristics of the PIZ may provide mechanistic insights into the development of life-threatening arrhythmias and sudden cardiac death post-MI. C1 [Schuleri, Karl H.; Centola, Marco; Evers, Kristine S.; Zviman, Adam; Evers, Robert; Lima, Joao A. C.; Lardo, Albert C.] Johns Hopkins Sch Med, Div Cardiol, Baltimore, MD 21205 USA. [Centola, Marco] Polo Univ, Azienda Osped San Paolo, Milan, Italy. [Evers, Robert] NIH, Bethesda, MD 20892 USA. [Lardo, Albert C.] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD USA. RP Lardo, AC (reprint author), Johns Hopkins Sch Med, Div Cardiol, 1042 Ross Bldg, Baltimore, MD 21205 USA. EM al@jhmi.edu FU NIH [U54 HL081028]; The Donald W. Reynolds foundation FX This work was supported by NIH grant U54 HL081028, and The Donald W. Reynolds foundation. The authors thank Norman J. Barker, M. S., R. B. P. for his expertise and helpful suggestions presenting images and data visually. NR 31 TC 18 Z9 18 U1 0 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1097-6647 J9 J CARDIOVASC MAGN R JI J. Cardiov. Magn. Reson. PD APR 17 PY 2012 VL 14 AR 24 DI 10.1186/1532-429X-14-24 PG 10 WC Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical Imaging SC Cardiovascular System & Cardiology; Radiology, Nuclear Medicine & Medical Imaging GA 942JC UT WOS:000304039200001 PM 22510220 ER PT J AU Rosamond, WD Chambless, LE Heiss, G Mosley, TH Coresh, J Whitsel, E Wagenknecht, L Ni, HY Folsom, AR AF Rosamond, Wayne D. Chambless, Lloyd E. Heiss, Gerardo Mosley, Thomas H. Coresh, Josef Whitsel, Eric Wagenknecht, Lynne Ni, Hanyu Folsom, Aaron R. TI Twenty-Two-Year Trends in Incidence of Myocardial Infarction, Coronary Heart Disease Mortality, and Case Fatality in 4 US Communities, 1987-2008 SO CIRCULATION LA English DT Article DE epidemiology; mortality rates; myocardial infarction; surveillance; survival ID ATHEROSCLEROSIS RISK; SURVEILLANCE; OUTCOMES; EPIDEMIOLOGY; ASSOCIATION; DEFINITION; CHALLENGES; EXPERIENCE; MINNESOTA; SEVERITY AB Background-Knowledge of trends in the incidence of and survival after myocardial infarction (MI) in a community setting is important to understanding trends in coronary heart disease (CHD) mortality rates. Methods and Results-We estimated race-and gender-specific trends in the incidence of hospitalized MI, case fatality, and CHD mortality from community-wide surveillance and validation of hospital discharges and of in-and out-of-hospital deaths among 35- to 74-year-old residents of 4 communities in the Atherosclerosis Risk in Communities (ARIC) Study. Biomarker adjustment accounted for change from reliance on cardiac enzymes to widespread use of troponin measurements over time. During 1987-2008, a total of 30 985 fatal or nonfatal hospitalized acute MI events occurred. Rates of CHD death among persons without a history of MI fell an average 4.7%/y among men and 4.3%/y among women. Rates of both in-and out-of-hospital CHD death declined significantly throughout the period. Age-and biomarker-adjusted average annual rate of incident MI decreased 4.3% among white men, 3.8% among white women, 3.4% among black women, and 1.5% among black men. Declines in CHD mortality and MI incidence were greater in the second decade (1997-2008). Failure to account for biomarker shift would have masked declines in incidence, particularly among blacks. Age-adjusted 28-day case fatality after hospitalized MI declined 3.5%/y among white men, 3.6%/y among black men, 3.0%/y among white women, and 2.6%/y among black women. Conclusions-Although these findings from 4 communities may not be directly generalizable to blacks and whites in the entire United States, we observed significant declines in MI incidence, primarily as a result of downward trends in rates between 1997 and 2008. (Circulation. 2012; 125: 1848-1857.) C1 [Rosamond, Wayne D.] Univ N Carolina, Dept Epidemiol, Cardiovasc Epidemiol Program, Sch Med, Chapel Hill, NC 27514 USA. [Chambless, Lloyd E.] Univ N Carolina, Sch Med, Dept Biostat, Gillings Sch Global Publ Hlth, Chapel Hill, NC 27514 USA. [Whitsel, Eric] Univ N Carolina, Sch Med, Dept Med, Chapel Hill, NC 27514 USA. [Mosley, Thomas H.] Univ Mississippi, Dept Med, Sch Med, Jackson, MS USA. [Coresh, Josef] Johns Hopkins Univ, Dept Epidemiol, Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Wagenknecht, Lynne] Wake Forest Univ, Bowman Gray Sch Med, Dept Publ Hlth Sci, Winston Salem, NC 27103 USA. [Ni, Hanyu] NHLBI, NIH, Bethesda, MD 20892 USA. [Folsom, Aaron R.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. RP Rosamond, WD (reprint author), Univ N Carolina, Dept Epidemiol, Cardiovasc Epidemiol Program, Sch Med, Bank Amer Bldg,137 Franklin St,Suite 306, Chapel Hill, NC 27514 USA. EM Wayne_Rosamond@unc.edu FU National Heart, Lung, and Blood Institute [N01-HC-55015, N01-HC-55016, N01-HC-55018, N01-HC-55019, N01-HC-55020, N01-HC-55021, N01-HC-55022] FX The ARIC Study is performed as a collaborative study supported by National Heart, Lung, and Blood Institute contracts N01-HC-55015, N01-HC-55016, N01-HC-55018, N01-HC-55019, N01-HC-55020, N01-HC-55021, and N01-HC-55022. NR 29 TC 107 Z9 111 U1 0 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD APR 17 PY 2012 VL 125 IS 15 BP 1848 EP + DI 10.1161/CIRCULATIONAHA.111.047480 PG 46 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 928CM UT WOS:000302957800012 PM 22420957 ER PT J AU Rosenson, RS Brewer, HB Davidson, WS Fayad, ZA Fuster, V Goldstein, J Hellerstein, M Jiang, XC Phillips, MC Rader, DJ Remaley, AT Rothblat, GH Tall, AR Yvan-Charvet, L AF Rosenson, Robert S. Brewer, H. Bryan, Jr. Davidson, W. Sean Fayad, Zahi A. Fuster, Valentin Goldstein, James Hellerstein, Marc Jiang, Xian-Cheng Phillips, Michael C. Rader, Daniel J. Remaley, Alan T. Rothblat, George H. Tall, Alan R. Yvan-Charvet, Laurent TI Cholesterol Efflux and Atheroprotection Advancing the Concept of Reverse Cholesterol Transport SO CIRCULATION LA English DT Review DE lipoproteins; apolipoproteins; atherosclerosis; cardiovascular diseases; imaging ID HIGH-DENSITY-LIPOPROTEIN; ESTER TRANSFER PROTEIN; PHOSPHOLIPID TRANSFER PROTEIN; APOLIPOPROTEIN-A-I; RECEPTOR CLASS-B; RANDOMIZED CONTROLLED-TRIAL; HEPATIC LIPASE DEFICIENCY; MARROW-DERIVED CELLS; PRE-BETA-HDL; SCAVENGER RECEPTOR C1 [Rosenson, Robert S.; Fayad, Zahi A.] Mt Sinai Sch Med, Translat & Mol Imaging Inst, New York, NY 10029 USA. [Brewer, H. Bryan, Jr.] Washington Hosp Ctr, Cardiovasc Res Inst, MedStar Res Inst, Washington, DC 20010 USA. [Davidson, W. Sean] Univ Cincinnati, Ctr Lipid & Arteriosclerosis Sci, Cincinnati, OH USA. [Goldstein, James] Oakland Univ, William Beaumont Sch Med, William Beaumont Hosp, Royal Oak, MI USA. [Hellerstein, Marc] Univ Calif Berkeley, Berkeley, CA 94720 USA. [Hellerstein, Marc] KineMed Inc, Berkeley, CA USA. [Hellerstein, Marc] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Jiang, Xian-Cheng] Suny Downstate Med Ctr, Brooklyn, NY 11203 USA. [Phillips, Michael C.; Rothblat, George H.] Univ Penn, Dept Pediat, Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Rader, Daniel J.] Univ Penn, Philadelphia, PA 19104 USA. [Remaley, Alan T.] NHLBI, NIH, Bethesda, MD 20892 USA. [Remaley, Alan T.; Yvan-Charvet, Laurent] Columbia Univ, Div Mol Med, Med Ctr, New York, NY USA. RP Rosenson, RS (reprint author), Mt Sinai Sch Med, Translat & Mol Imaging Inst, 1 Gustave L Levy Pl,Box 1030, New York, NY 10029 USA. EM robert.rosenson@mssm.edu RI Fuster, Valentin/H-4319-2015; OI Fuster, Valentin/0000-0002-9043-9986; Yvan-Charvet, Laurent/0000-0002-7748-4942 FU Intramural NIH HHS [ZIA HL006095-03] NR 147 TC 297 Z9 308 U1 6 U2 46 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD APR 17 PY 2012 VL 125 IS 15 BP 1905 EP 1919 DI 10.1161/CIRCULATIONAHA.111.066589 PG 15 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 928CM UT WOS:000302957800018 PM 22508840 ER PT J AU Kunisue, T Chen, Z Louis, GMB Sundaram, R Hediger, ML Sun, LP Kannan, K AF Kunisue, Tatsuya Chen, Zhen Louis, Germaine M. Buck Sundaram, Rajeshwari Hediger, Mary L. Sun, Liping Kannan, Kurunthachalam TI Urinary Concentrations of Benzophenone-type UV Filters in U.S. Women and Their Association with Endometriosis SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID TANDEM MASS-SPECTROMETRY; ISOLATED RAT HEPATOCYTES; YEAST 2-HYBRID ASSAY; SYSTEMIC ABSORPTION; ESTROGENIC ACTIVITY; IN-VITRO; POLYCHLORINATED-BIPHENYLS; TOPICAL APPLICATION; MCF-7 CELLS; SUNSCREEN AB Benzophenone (BP)-type UV filters are widely used in a variety of personal care products for the protection of skin and hair from UV irradiation. Despite the estrogenic potencies of BP derivatives, few studies have examined the occurrence of these compounds in human matrices. Furthermore, associations among exposure to these compounds and estrogen-dependent diseases (such as endometriosis) have not been examined previously. In this study, we determined the concentrations of five BP derivatives, 2-hydroxy-4-methoxybenzophenone (2OH-4MeO-BP), 2,4-dihydroxybenzophenone (2,4OH-BP), 2,2'-dihydroxy-4-methoxybenzophenone (2,2'OH-4MeO-BP), 2,2',4,4'-tetrahydroxybenzophenone (2,2',4,4'OH-BP), and 4-hydroxybenzophenone (4OH-BP), in urine collected from 625 women in Utah and California, using liquid chromatography (LC)-tandem mass spectrometry (MS/MS). The association of urinary concentrations of BP derivatives with an increase in the odds of a diagnosis of endometriosis was examined in 600 women who underwent laparoscopy/laparotomy (n = 473: operative cohort) or pelvic magnetic resonance imaging (n = 127: population cohort), during 2007-2009. 2OH-4MeO-BP, 2,4OH-BP, and 4OH-BP respectively were detected in 99.0%, 93.3%, and 83.8% of the urine samples analyzed, whereas the detection rates for 2,2',4,4'OH-BP and 2,2'OH-4MeO-BP were below 6.0%. Significant regional differences (higher concentrations in California) and monthly variations (higher concentrations in July and August) were found for urinary concentrations of 2OH-4MeO-BP and 2,4OH-BP. In addition, urinary concentrations of 2OH-4MeO-BP and 2,4OH-BP tended to be higher in more affluent, older, and leaner women. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated for the urinary concentrations of BP derivatives and the odds of an endometriosis diagnosis; ORs increased across quartiles of 2OH-4MeO-BP and 2,4OH-BP concentrations, but a significant trend was observed only between 2,4OH-BP and the odds of an endometriosis diagnosis in the operative cohort (OR = 1.19; 95% CI = 1.01, 1.41). When women in the highest quartile of 2,4OH-BP concentrations were compared with women in the first three quartiles, the OR increased considerably (OR = 1.65; 95% CI = 1.07, 2.53). Given that 2,4OH-BP possesses an estrogenic activity higher than that of 2OH-4MeO-BP, our results invite the speculation that exposure to elevated 2,4OH-BP levels may be associated with endometriosis. C1 [Kunisue, Tatsuya; Kannan, Kurunthachalam] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA. [Kunisue, Tatsuya; Kannan, Kurunthachalam] SUNY Albany, Sch Publ Hlth, Dept Environm Hlth Sci, Albany, NY 12201 USA. [Chen, Zhen; Louis, Germaine M. Buck; Sundaram, Rajeshwari; Hediger, Mary L.; Sun, Liping] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Epidemiol Stat & Prevent Res, NIH, Rockville, MD 20852 USA. [Kannan, Kurunthachalam] Harbin Inst Technol, IJRC PTS, State Key Lab Urban Water Resources & Environm, Harbin 150090, Peoples R China. RP Kannan, K (reprint author), New York State Dept Hlth, Wadsworth Ctr, Empire State Plaza,POB 509, Albany, NY 12201 USA. EM kkannan@wadsworth.org RI Kunisue, Tatsuya/G-4171-2014; OI Kunisue, Tatsuya/0000-0002-8167-1564; Sundaram, Rajeshwari/0000-0002-6918-5002; Buck Louis, Germaine/0000-0002-1774-4490 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health [NO1-DK-6-3428, NO1-DK-6-3427, 10001406-02] FX This study was funded by the Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (contracts NO1-DK-6-3428, NO1-DK-6-3427, 10001406-02). NR 43 TC 88 Z9 91 U1 13 U2 76 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD APR 17 PY 2012 VL 46 IS 8 BP 4624 EP 4632 DI 10.1021/es204415a PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 926SB UT WOS:000302850300050 PM 22417702 ER PT J AU Wendler, D AF Wendler, David TI Consent for Research With Biological Samples: One-Time General Consent Versus a Gift Model SO ANNALS OF INTERNAL MEDICINE LA English DT Editorial Material ID INFORMED-CONSENT; GENETIC RESEARCH; TRIBE C1 NIH, Ctr Clin, Dept Bioeth, Bethesda, MD 20892 USA. RP Wendler, D (reprint author), NIH, Ctr Clin, Dept Bioeth, Bldg 10,Room 1C118, Bethesda, MD 20892 USA. EM dwendler@nih.gov NR 18 TC 3 Z9 3 U1 0 U2 2 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 EI 1539-3704 J9 ANN INTERN MED JI Ann. Intern. Med. PD APR 17 PY 2012 VL 156 IS 8 BP 596 EP U122 DI 10.7326/0003-4819-156-8-201204170-00008 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA 930PO UT WOS:000303151800019 PM 22508735 ER PT J AU Khademi, H Malekzadeh, R Pourshams, A Jafari, E Salahi, R Semnani, S Abaie, B Islami, F Nasseri-Moghaddam, S Etemadi, A Byrnes, G Abnet, CC Dawsey, SM Day, NE Pharoah, PD Boffetta, P Brennan, P Kamangar, F AF Khademi, Hooman Malekzadeh, Reza Pourshams, Akram Jafari, Elham Salahi, Rasool Semnani, Shahryar Abaie, Behrooz Islami, Farhad Nasseri-Moghaddam, Siavosh Etemadi, Arash Byrnes, Graham Abnet, Christian C. Dawsey, Sanford M. Day, Nicholas E. Pharoah, Paul D. Boffetta, Paolo Brennan, Paul Kamangar, Farin TI Opium use and mortality in Golestan Cohort Study: prospective cohort study of 50 000 adults in Iran SO BRITISH MEDICAL JOURNAL LA English DT Article ID HIGH-RISK AREA; ESOPHAGEAL CANCER; SOCIOECONOMIC-STATUS; BLADDER-CANCER; MORPHINE; RELIABILITY; POPULATION; METABOLISM; ADDICTION; CARCINOMA AB Objectives To investigate the association between opium use and subsequent risk of death. Design Prospective cohort study. Setting The Golestan Cohort Study in north-eastern Iran collected detailed validated data on opium use and other exposures at baseline. Participants were enrolled between January 2004 and June 2008 and were followed to May 2011, with a follow-up success rate of over 99%. Participants 50 045 participants aged 40-75 at baseline. Main outcomes Mortality, all cause and major subcategories. Results 17% (n=8487) of the participants reported opium use, with a mean duration of 12.7 years. During the follow-up period 2145 deaths were reported. The adjusted hazard ratio for all cause mortality associated with ever use of opium was 1.86 (95% confidence interval 1.68 to 2.06). Opium consumption was significantly associated with increased risks of deaths from several causes including circulatory diseases (hazard ratio 1.81) and cancer (1.61). The strongest associations were seen with deaths from asthma, tuberculosis, and chronic obstructive pulmonary disease (11.0, 6.22, and 5.44, respectively). After exclusion of people who self prescribed opium after the onset of major chronic illnesses, the associations remained strong with a dose-response relation. Conclusion Opium users have an increased risk of death from multiple causes compared with non-users. Increased risks were also seen in people who used low amounts of opium for a long period and those who had no major illness before use. C1 [Khademi, Hooman; Malekzadeh, Reza; Pourshams, Akram; Jafari, Elham; Abaie, Behrooz; Islami, Farhad; Nasseri-Moghaddam, Siavosh; Etemadi, Arash; Kamangar, Farin] Univ Tehran Med Sci, Digest Dis Res Ctr, Shariati Hosp, Tehran, Iran. [Khademi, Hooman; Byrnes, Graham; Brennan, Paul] Int Agcy Res Canc, F-69372 Lyon, France. [Salahi, Rasool; Semnani, Shahryar] Golestan Univ Med Sci, Golestan Res Ctr Gastroenterol & Hepatol, Gorgan, Iran. [Islami, Farhad; Boffetta, Paolo] Mt Sinai Sch Med, Inst Translat Epidemiol, New York, NY USA. [Islami, Farhad; Boffetta, Paolo] Mt Sinai Sch Med, Tisch Canc Inst, New York, NY USA. [Etemadi, Arash; Abnet, Christian C.; Dawsey, Sanford M.; Kamangar, Farin] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Day, Nicholas E.; Pharoah, Paul D.] Univ Cambridge, Dept Oncol & Publ Hlth, Cambridge, England. [Day, Nicholas E.; Pharoah, Paul D.] Univ Cambridge, Dept Primary Care, Cambridge, England. [Boffetta, Paolo] Int Prevent Res Inst, Lyon, France. [Kamangar, Farin] Morgan State Univ, Dept Publ Hlth Anal, Sch Community Hlth & Policy, Baltimore, MD 21239 USA. RP Malekzadeh, R (reprint author), Univ Tehran Med Sci, Digest Dis Res Ctr, Shariati Hosp, Tehran, Iran. EM malek@ams.ac.ir; brennan@iarc.fr RI Abnet, Christian/C-4111-2015; Etemadi, Arash/C-1386-2016; Semnani, Shahryar/N-2270-2016; OI Abnet, Christian/0000-0002-3008-7843; Etemadi, Arash/0000-0002-3458-1072; Semnani, Shahryar/0000-0002-8768-6142; Malekzadeh, Reza/0000-0003-1043-3814 FU Tehran University of medical sciences [81/15]; Cancer Research UK [C20/A5860]; NCI, National Institutes of Health; IARC; Social Security Organization of Iran Golestan Branch FX The Golestan Cohort Study was supported by Tehran University of medical sciences (grant No: 81/15), Cancer Research UK (grant No: C20/A5860), the Intramural Research Program of the NCI, National Institutes of Health, and through various collaborative research agreements with the IARC. The study has also received special support from the Social Security Organization of Iran Golestan Branch. NR 41 TC 39 Z9 39 U1 2 U2 10 PU B M J PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1756-1833 J9 BRIT MED J JI Br. Med. J. PD APR 17 PY 2012 VL 344 AR e2502 DI 10.1136/bmj.e2502 PG 12 WC Medicine, General & Internal SC General & Internal Medicine GA 931CN UT WOS:000303192100003 PM 22511302 ER PT J AU Gravotta, D Carvajal-Gonzalez, JM Mattera, R Deborde, S Banfelder, JR Bonifacino, JS Rodriguez-Boulan, E AF Gravotta, Diego Carvajal-Gonzalez, Jose Maria Mattera, Rafael Deborde, Sylvie Banfelder, Jason R. Bonifacino, Juan S. Rodriguez-Boulan, Enrique TI The Clathrin Adaptor AP-1A Mediates Basolateral Polarity SO DEVELOPMENTAL CELL LA English DT Article ID MANNOSE 6-PHOSPHATE RECEPTORS; TRANS-GOLGI NETWORK; EPITHELIAL-CELLS; MDCK CELLS; STRUCTURAL EXPLANATION; TRANSFERRIN RECEPTOR; RECYCLING ENDOSOMES; CYTOPLASMIC DOMAIN; MEMBRANE DOMAINS; PLASMA-MEMBRANE AB Clathrin and the epithelial-specific clathrin adaptor AP-1B mediate basolateral trafficking in epithelia. However, several epithelia lack AP-1B, and mice knocked out for AP-1B are viable, suggesting the existence of additional mechanisms that control basolateral polarity. Here, we demonstrate a distinct role of the ubiquitous clathrin adaptor AP-1A in basolateral protein sorting. Knockdown of AP-1A causes missorting of basolateral proteins in MDCK cells, but only after knockdown of AP-1B, suggesting that AP-1B can compensate for lack of AP-1A. AP-1A localizes predominantly to the TGN, and its knockdown promotes spillover of basolateral proteins into common recycling endosomes, the site of function of AP-1B, suggesting complementary roles of both adaptors in basolateral sorting. Yeast two-hybrid assays detect interactions between the basolateral signal of transferrin receptor and the medium subunits of both AP-1A and AP-1B. The basolateral sorting function of AP-1A reported here establishes AP-1 as a major regulator of epithelial polarity. C1 [Gravotta, Diego; Carvajal-Gonzalez, Jose Maria; Deborde, Sylvie; Rodriguez-Boulan, Enrique] Weill Cornell Med Coll, Dept Ophthalmol, Margaret Dyson Vis Res Inst, New York, NY 10065 USA. [Banfelder, Jason R.] Weill Cornell Med Coll, Dept Physiol & Biophys, New York, NY 10065 USA. [Rodriguez-Boulan, Enrique] Weill Cornell Med Coll, Dept Cell & Dev Biol, New York, NY 10065 USA. [Mattera, Rafael; Bonifacino, Juan S.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cell Biol & Metab Program, NIH, Bethesda, MD 20892 USA. RP Gravotta, D (reprint author), Weill Cornell Med Coll, Dept Ophthalmol, Margaret Dyson Vis Res Inst, New York, NY 10065 USA. EM dig2003@med.cornell.edu; boulan@med.cornell.edu OI Deborde, Sylvie/0000-0002-6630-4029; Carvajal-Gonzalez, Jose Maria/0000-0001-6576-830X; Bonifacino, Juan S./0000-0002-5673-6370 FU National Institutes of Health (NIH) [GM34108, EY08538]; Research to Prevent Blindness Foundation; Dyson Foundation; EMBO; Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH FX We thank Dr. Alfonso Gonzalez for reading and commenting on the manuscript. This work was supported by National Institutes of Health (NIH) grants GM34108 and EY08538 to E.R.-B., by funds from Research to Prevent Blindness Foundation, by the Dyson Foundation, and by an EMBO Postdoctoral Fellowship to J.M.C.-G. R.M. and J.S.B. were supported by the Intramural Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH. We thank B. Lelouvier (National Heart, Lung, and Blood Institute, NIH) for providing an ImageJ plugin for automated analysis of confocal images; Drs. Andres Perez-Bay, Ryan Schreiner, and Susana Salvarezza for colocalization experiments; and Luciana Gravotta, M.A., for help with statistical analyses. NR 59 TC 75 Z9 76 U1 0 U2 6 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1534-5807 J9 DEV CELL JI Dev. Cell PD APR 17 PY 2012 VL 22 IS 4 BP 811 EP 823 DI 10.1016/j.devcel.2012.02.004 PG 13 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 929XQ UT WOS:000303099400015 PM 22516199 ER PT J AU Cha, YR Fujita, M Butler, M Isogai, S Kochhan, E Siekmann, AF Weinstein, BM AF Cha, Young Ryun Fujita, Misato Butler, Matthew Isogai, Sumio Kochhan, Eva Siekmann, Arndt F. Weinstein, Brant M. TI Chemokine Signaling Directs Trunk Lymphatic Network Formation along the Preexisting Blood Vasculature SO DEVELOPMENTAL CELL LA English DT Article ID INHIBITS SPROUTING ANGIOGENESIS; ENDOTHELIAL-GROWTH-FACTOR; DEVELOPING ZEBRAFISH; LATERAL-LINE; IN-VIVO; TRANSGENIC ZEBRAFISH; CXCR4 ANTAGONISTS; TISSUE MIGRATION; CELL-MIGRATION; RECEPTOR CXCR4 AB The lymphatic system is crucial for fluid homeostasis, immune responses, and numerous pathological processes. However, the molecular mechanisms responsible for establishing the anatomical form of the lymphatic vascular network remain largely unknown. Here, we show that chemokine signaling provides critical guidance cues directing early trunk lymphatic network assembly and patterning. The chemokine receptors Cxcr4a and Cxcr4b are expressed in lymphatic endothelium, whereas chemokine ligands Cxcl12a and Cxcl12b are expressed in adjacent tissues along which the developing lymphatics align. Loss- and gain-of-function studies in zebrafish demonstrate that chemokine signaling orchestrates the stepwise assembly of the trunk lymphatic network. In addition to providing evidence for a lymphatic vascular guidance mechanism, these results also suggest a molecular basis for the anatomical coalignment of lymphatic and blood vessels. C1 [Cha, Young Ryun; Fujita, Misato; Butler, Matthew; Weinstein, Brant M.] NICHHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. [Isogai, Sumio] Iwate Med Univ, Dept Anat, Sch Med, Morioka, Iwate 0208505, Japan. [Kochhan, Eva; Siekmann, Arndt F.] Max Planck Inst Mol Biomed, D-48149 Munster, Germany. RP Weinstein, BM (reprint author), NICHHD, Mol Genet Lab, NIH, 6B-309,6 Ctr Dr, Bethesda, MD 20892 USA. EM bw96w@nih.gov FU Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Institutes of Health; Leducq Foundation; Max Planck Society; Deutsche Forschungsgemeinschaft [SI 1374/3-1]; ERC [260794-ZebrafishAngio] FX We thank members of the Weinstein laboratory for technical help and critical suggestions. We would like to thank Dr. Nobutaka Fujii for providing the Cxcr4 inhibitor (TF10416). We are also grateful to James Chen for the gift of GV-EcRF' plasmid. This research was supported by intramural program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development and National Institutes of Health and by the Leducq Foundation. A.F.S. was supported by the Max Planck Society, a grant from the Deutsche Forschungsgemeinschaft (SI 1374/3-1), and an ERC starting grant (260794-ZebrafishAngio). NR 64 TC 42 Z9 42 U1 0 U2 8 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1534-5807 J9 DEV CELL JI Dev. Cell PD APR 17 PY 2012 VL 22 IS 4 BP 824 EP 836 DI 10.1016/j.devcel.2012.01.011 PG 13 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 929XQ UT WOS:000303099400016 PM 22516200 ER PT J AU Valley, CT Porter, DF Qiu, C Campbell, ZT Hall, TMT Wickens, M AF Valley, Cary T. Porter, Douglas F. Qiu, Chen Campbell, Zachary T. Hall, Traci M. Tanaka Wickens, Marvin TI Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE mRNA turnover; RNA regulation; translation; 3 ' UTR elements ID PUMILIO-HOMOLOGY DOMAIN; YEAST 3-HYBRID SYSTEM; ELEGANS PUF PROTEIN; MESSENGER-RNA; BINDING PROTEIN; CAENORHABDITIS-ELEGANS; MITOCHONDRIAL BIOGENESIS; WIDE IDENTIFICATION; IN-VIVO; FAMILY AB mRNA control hinges on the specificity and affinity of proteins for their RNA binding sites. Regulatory proteins must bind their own sites and reject even closely related noncognate sites. In the PUF [Pumilio and fem-3 binding factor (FBF)] family of RNA binding proteins, individual proteins discriminate differences in the length and sequence of binding sites, allowing each PUF to bind a distinct battery of mRNAs. Here, we show that despite these differences, the pattern of RNA interactions is conserved among PUF proteins: the two ends of the PUF protein make critical contacts with the two ends of the RNA sites. Despite this conserved "two-handed" pattern of recognition, the RNA sequence is flexible. Among the binding sites of yeast Puf4p, RNA sequence dictates the pattern in which RNA bases are flipped away from the binding surface of the protein. Small differences in RNA sequence allow new modes of control, recruiting Puf5p in addition to Puf4p to a single site. This embedded information adds a new layer of biological meaning to the connections between RNA targets and PUF proteins. C1 [Campbell, Zachary T.; Wickens, Marvin] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA. [Valley, Cary T.] Univ Wisconsin, Grad Program Cellular & Mol Biol, Madison, WI 53706 USA. [Porter, Douglas F.] Univ Wisconsin, Integrated Program Biochem, Madison, WI 53706 USA. [Qiu, Chen; Hall, Traci M. Tanaka] Natl Inst Environm Hlth Sci, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. RP Wickens, M (reprint author), Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA. EM wickens@biochem.wisc.edu FU National Institutes of Health [GM50942, GM095169]; National Institute of Environmental Health Sciences; National Institutes of Health/National Institute of General Medical Sciences [5T32GM08349]; US Department of Energy, Office of Science, Office of Basic Energy Sciences [W-31-109-Eng-38] FX We thank Dr. Lars Pedersen and the staff at the Southeast Regional Collaborative Access Team beamline for help with X-ray data collection and Dr. Vanderploeg for help with figures. Data were collected at Southeast Regional Collaborative Access Team 22-ID beamline at the Advanced Photon Source, Argonne National Laboratory. Supporting institutions may be found at www.ser-cat.org/members.html. This work was supported by Grants GM50942 and GM095169 from the National Institutes of Health (to M. W. and Z.T.C. respectively), by a grant from the Intramural Research Program of the National Institute of Environmental Health Sciences (to T. M. T. H.), and by National Institutes of Health/National Institute of General Medical Sciences Grant 5T32GM08349 (to D. F. P.). Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract W-31-109-Eng-38. NR 50 TC 19 Z9 21 U1 1 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6054 EP 6059 DI 10.1073/pnas.1200521109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100036 PM 22467831 ER PT J AU Malik, M Jividen, K Padmakumar, VC Cataisson, C Li, LW Lee, J Howard, OMZ Yuspa, SH AF Malik, Mariam Jividen, Kasey Padmakumar, V. C. Cataisson, Christophe Li, Luowei Lee, Jessica Howard, O. M. Zack Yuspa, Stuart H. TI Inducible NOS-induced chloride intracellular channel 4 (CLIC4) nuclear translocation regulates macrophage deactivation SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE protein nitrosylation; IL-1beta; phagocytosis ID GROWTH-FACTOR-BETA; NITRIC-OXIDE; RECEPTOR EXPRESSION; MOUSE MACROPHAGES; PROTEIN CLIC4; ION CHANNEL; ACTIVATION; INFLAMMATION; LIPOPOLYSACCHARIDE; MTCLIC/CLIC4 AB Nuclear translocation of cytosolic CLIC4 is an essential feature of its proapoptotic and prodifferentiation functions. Here we demonstrate that CLIC4 is induced concurrently with inducible nitric oxide synthase (iNOS) and S-nitrosylated in proinflammatory peritoneal macrophages. Chemical inhibition or genetic ablation of iNOS inhibits S-nitrosylation and nuclear translocation of CLIC4. In macrophages, iNOS-induced nuclear CLIC4 coincides with the proto anti-inflammatory transition of the cells because IL-1 beta and CXCL1 mRNA remain elevated in CLIC4 and iNOS knockout macrophages at late time points, whereas TNF alpha mRNA is elevated only in the iNOS knockout macrophages. Active IL-1 beta remains elevated in CLIC4 knockout macrophages and in macrophages in which CLIC4 nuclear translocation is prevented by the NOS inhibitor L-NAME. Moreover, overexpression of nuclear-targeted CLIC4 down-regulates IL-1 beta in stimulated macrophages. In mice, genetically null for CLIC4, the number of phagocytosing macrophages stimulated by LPS is reduced. Thus, iNOS-induced nuclear CLIC4 is an essential part of the macrophage deactivation program. C1 [Malik, Mariam; Jividen, Kasey; Padmakumar, V. C.; Cataisson, Christophe; Li, Luowei; Lee, Jessica; Yuspa, Stuart H.] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. [Howard, O. M. Zack] NCI, Mol Immunoregulat Lab, Ctr Canc Res, Bethesda, MD 20892 USA. RP Yuspa, SH (reprint author), NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. EM yuspas@mail.nih.gov RI Howard, O M Zack/B-6117-2012; OI Howard, O M Zack/0000-0002-0505-7052; Jividen, Kasey/0000-0003-1022-5038 FU National Cancer Institute; National Institutes of Health FX We thank Kathleen Noer and Roberta Matthai of the Cancer and Inflammation Program Fluorescence-Activated Cell Sorter Core for technical assistance in immune cell sorting; Susan Garfield and the National Cancer Institute Confocal Core for microscopy assistance; and Marta Custer for maintenance of animal colonies. We also thank Anjali Shukla and Francesca Mascia for helpful suggestions. This work was supported by the intramural program of the National Cancer Institute and the National Institutes of Health. NR 35 TC 13 Z9 13 U1 2 U2 7 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6130 EP 6135 DI 10.1073/pnas.1201351109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100049 PM 22474389 ER PT J AU Yu, P Steel, JC Zhang, ML Morris, JC Waitz, R Fasso, M Allison, JP Waldmann, TA AF Yu, Ping Steel, Jason C. Zhang, Meili Morris, John C. Waitz, Rebecca Fasso, Marcella Allison, James P. Waldmann, Thomas A. TI Simultaneous inhibition of two regulatory T-cell subsets enhanced Interleukin-15 efficacy in a prostate tumor model SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID GROWTH-FACTOR-BETA; CUTTING EDGE; LUNG-CANCER; CTLA-4; IL-10; TOLERANCE; PD-1; PROLIFERATION; RESPONSES; IMMUNITY AB IL-15 has potential as an immunotherapeutic agent for cancer treatment because of its ability to effectively stimulate CD8 T cell, natural killer T cell, and natural killer cell immunity. However, its effectiveness may be limited by negative immunological checkpoints that attenuate immune responses. Recently a clinical trial of IL-15 in cancer immunotherapy was initiated. Finding strategies to conquer negative regulators and enhance efficacy of IL-15 is critical and meaningful for such clinical trials. In a preclinical study, we evaluated IL-15 combined with antibodies to block negative immune regulator cytotoxic T lymphocyte antigen 4 (CTLA-4) and programmed death ligand 1 (PD-L1) in an established murine transgenic adenocarcinoma of mouse prostate (TRAMP)-C2 prostate tumor model. IL-15 treatment resulted in a significant prolongation of survival in tumor-bearing animals. Coadministration of anti-PD-L1 or anti-CTLA-4 singly with IL-15 did not improve animal survival over that of IL-15 alone. However, simultaneous administration of IL-15 with anti-CTLA-4 and anti-PD-L1 was associated with increased numbers of tumor antigen-specific tetramer-positive CD8 T cells, increased CD8T-cell tumor lytic activity, augmented antigen-specific IFN-gamma release, decreased rates of tumor growth, and improved animal survival compared with IL-15 alone. Furthermore, triple combination therapy was associated with inhibition of suppressive functions of CD4(+)CD25(+) regulatory T cells and CD8(+)CD122(+) regulatory T cells. Thus, simultaneous blockade of CTLA-4 and PD-L1 protected CD4 and/or CD8 T-cell activity from these regulatory T cells. Combining the immune stimulatory properties of IL-15 with simultaneous removal of two critical immune inhibitory checkpoints, we showed enhancement of immune responses, leading to increased antitumor activity. C1 [Yu, Ping; Steel, Jason C.; Zhang, Meili; Morris, John C.; Waldmann, Thomas A.] NCI, Metab Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Steel, Jason C.; Morris, John C.] Univ Cincinnati, Div Hematol Oncol, Cincinnati, OH 45267 USA. [Zhang, Meili] NCI, Lab Anim Sci Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Waitz, Rebecca; Allison, James P.] Mem Sloan Kettering Canc Ctr, Howard Hughes Med Inst, Dept Immunol, New York, NY 10065 USA. [Fasso, Marcella] Univ Calif San Francisco, Dept Urol, San Francisco, CA 94193 USA. RP Waldmann, TA (reprint author), NCI, Metab Branch, Ctr Canc Res, Bethesda, MD 20892 USA. EM tawald@helix.nih.gov RI Steel, Jason/D-1805-2013 OI Steel, Jason/0000-0003-3608-7542 FU National Institutes of Health, National Cancer Institute, Center for Cancer Research; Howard Hughes Medical Institute, Ludwig Center of Cancer Immunotherapy, Prostate Cancer Foundation FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research (P.Y., J.C.S., M.Z., J.C.M., and T. A. W.). R. W., M. F., and J.P.A. were funded by the Howard Hughes Medical Institute, Ludwig Center of Cancer Immunotherapy, Prostate Cancer Foundation. NR 34 TC 27 Z9 28 U1 1 U2 10 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6187 EP 6192 DI 10.1073/pnas.1203479109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100059 PM 22474386 ER PT J AU Schoch, CL Seifert, KA Huhndorf, S Robert, V Spouge, JL Levesque, CA Chen, W Bolchacova, E Voigt, K Crous, PW Miller, AN Wingfield, MJ Aime, MC An, KD Bai, FY Barreto, RW Begerow, D Bergeron, MJ Blackwell, M Boekhout, T Bogale, M Boonyuen, N Burgaz, AR Buyck, B Cai, L Cai, Q Cardinali, G Chaverri, P Coppins, BJ Crespo, A Cubas, P Cummings, C Damm, U de Beer, ZW de Hoog, GS Del-Prado, R Dentinger B Dieguez-Uribeondo, J Divakar, PK Douglas, B Duenas, M Duong, TA Eberhardt, U Edwards, JE Elshahed, MS Fliegerova, K Furtado, M Garcia, MA Ge, ZW Griffith, GW Griffiths, K Groenewald, JZ Groenewald, M Grube, M Gryzenhout, M Guo, LD Hagen, F Hambleton, S Hamelin, RC Hansen, K Harrold, P Heller, G Herrera, G Hirayama, K Hirooka, Y Ho, HM Hoffmann, K Hofstetter, V Hognabba, F Hollingsworth, PM Hong, SB Hosaka, K Houbraken, J Hughes, K Huhtinen, S Hyde, KD James, T Johnson, EM Johnson, JE Johnston, PR Jones, EB Kelly, LJ Kirk, PM Knapp, DG Koljalg, U Kovacs GM Kurtzman, CP Landvik, S Leavitt, SD Liggenstoffer, AS Liimatainen, K Lombard, L Luangsa-Ard, JJ Lumbsch, HT Maganti, H Maharachchikumbura, SS Martin, MP May, TW McTaggart, AR Methven, AS Meyer, W Moncalvo, JM Mongkolsamrit, S Nagy, LG Nilsson, RH Niskanen, T Nyilasi, I Okada, G Okane, I Olariaga, I Otte, J Papp, T Park, D Petkovits, T Pino-Bodas, R Quaedvlieg, W Raja, HA Redecker, D Rintoul T Ruibal, C Sarmiento-Ramirez, JM Schmitt, I Schussler, A Shearer, C Sotome, K Stefani, FO Stenroos, S Stielow, B Stockinger, H Suetrong, S Suh, SO Sung, GH Suzuki, M Tanaka, K Tedersoo, L Telleria, MT Tretter, E Untereiner, WA Urbina, H Vagvolgyi, C Vialle, A Vu, TD Walther, G Wang, QM Wang, Y Weir, BS Weiss, M White, MM Xu, J Yahr, R Yang, ZL Yurkov, A Zamora, JC Zhang, N Zhuang, WY Schindel, D AF Schoch, Conrad L. Seifert, Keith A. Huhndorf, Sabine Robert, Vincent Spouge, John L. Levesque, C. Andre Chen, Wen Bolchacova, E. Voigt, K. Crous, P.W. Miller, A.N. Wingfield, M.J. Aime, M.C. An, K.D. Bai, F.Y Barreto, R.W. Begerow, D. Bergeron, MJ Blackwell, M Boekhout, T Bogale, M Boonyuen, N Burgaz, AR Buyck, B Cai, L Cai, Q Cardinali, G Chaverri, P Coppins, BJ Crespo, A Cubas P, P. Cummings, C Damm, U. de Beer, Z.W. de Hoog, G.S. Del-Prado, R Dentinger, B. Dieguez-Uribeondo, J Divakar, PK Douglas, B Duenas, M Duong, T.A. Eberhardt, U Edwards, J.E. Elshahed, MS Fliegerova, K Furtado, M. Garcia, M.A. Ge, Z.W. Griffith, GW Griffiths, K Groenewald, JZ Groenewald, M Grube, M Gryzenhout, M Guo, LD Hagen, F Hambleton, S Hamelin, RC Hansen, K Harrold, P Heller, G Herrera, G Hirayama, K Hirooka, Y Ho, H.M. Hoffmann, K. Hofstetter, V Hognabba, F. Hollingsworth, P.M. Hong, S.B. Hosaka, K Houbraken, J Hughes, K Huhtinen, S. Hyde, KD James, T Johnson, EM Johnson, JE Johnston, PR Jones, EB Kelly, L.J. Kirk, PM Knapp, DG Koljalg, U Kovacs, GM Kurtzman, CP Landvik, S Leavitt, SD Liggenstoffer, AS Liimatainen, K. Lombard, L. Luangsa-Ard, J.J. Lumbsch, HT Maganti H Maharachchikumbura, SS Martin, MP May, TW McTaggart, A.R. Methven, AS Meyer, W Moncalvo, J.M. Mongkolsamrit, S Nagy, L.G. Nilsson, RH Niskanen, T Nyilasi, I Okada, G Okane, I Olariaga, I Otte, J. Papp, T Park, D Petkovits, T Pino-Bodas, R. Quaedvlieg, W Raja, H.A. Redecker, D. Rintoul, T. Ruibal, C. Sarmiento-Ramirez, J.M. Schmitt, I Schussler, A. Shearer, C. Sotome, K. Stefani, FO Stenroos, S. Stielow, B. Stockinger, H. Suetrong, S. Suh, S.O. Sung, G.H. Suzuki, M. Tanaka, K. Tedersoo, L. Telleria, MT Tretter, E. Untereiner, W.A. Urbina, H. Vagvolgyi, C Vialle, A Vu, T.D. Walther, G. Wang, Q.M. Wang, Y. Weir, B.S. Weiss, M. White, M.M. Xu, J. Yahr, R. Yang, Z.L. Yurkov, A. Zamora, J.C. Zhang, N. Zhuang, W.Y. Schindel, D CA Fungal Barcoding Consortium TI Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE DNA barcoding; fungal biodiversity ID ARBUSCULAR MYCORRHIZAL FUNGI; PHYLOGENETIC-RELATIONSHIPS; BASIDIOMYCETOUS YEASTS; INTRAGENOMIC VARIATION; ECTOMYCORRHIZAL FUNGI; SPECIES RECOGNITION; SEQUENCE-ANALYSIS; RPB1 SEQUENCES; RDNA; SUBUNIT AB Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter-and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups. C1 [Schoch, Conrad L.; Spouge, John L.; Fungal Barcoding Consortium] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA. [Seifert, Keith A.; Levesque, C. Andre; Chen, Wen] Agr & Agri Food Canada, Biodivers Mycol & Microbiol, Ottawa, ON K1A 0C6, Canada. [Huhndorf, Sabine] Field Museum, Dept Bot, Chicago, IL 60605 USA. [Robert, Vincent] Cent Bur Schimmelcultures Fungal Biodivers Ctr CB, NL-3508 AD Utrecht, Netherlands. RP Schoch, CL (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bldg 10, Bethesda, MD 20892 USA. EM schoch2@ncbi.nlm.nih.gov; Keith.Seifert@AGR.GC.CA RI Quaedvlieg, William/C-9449-2013; Yurkov, Andrey/E-7854-2011; Cai, Lei/D-1589-2013; CARDINALI, GIANLUIGI/B-8622-2011; McTaggart, Alistair/A-8871-2014; May, Tom/C-2460-2014; Fliegerova, Katerina/G-7196-2014; CAI, LEI/K-7226-2014; Hagen, Ferry/B-9044-2009; Meyer, Wieland/G-1204-2015; Telleria, M. Teresa/H-4928-2015; Duenas, Margarita/H-4944-2015; SchuSSler, Arthur/F-1681-2011; Groenewald, Johannes/F-4667-2011; Crous, Pedro/H-1489-2012; Tedersoo, Leho/H-3541-2012; Weir, Bevan/B-5057-2008; Duong, Tuan/I-9432-2012; Schoch, Conrad/J-4825-2012; Lumbsch, Thorsten/K-3573-2012; Zhang, Ning/K-3046-2012; Wingfield, Michael/A-9473-2008; Lombard, Lorenzo/B-2042-2010; Boonyuen, Nattawut/F-2167-2011; WeiSS, Michael/F-6206-2012; Groenewald, Marizeth/G-4976-2012; de Beer, Z. Wilhelm/B-6353-2008; Knapp, Daniel/C-9481-2013; Maharachchikumbura, Sajeewa/C-9403-2013; Garcia, Miguel Angel/N-6275-2016; Martin, Maria /H-8069-2012; Divakar, Pradeep/E-5603-2014; OI Stenroos, Soili/0000-0003-0331-792X; Grube, Martin/0000-0001-6940-5282; Nilsson, Henrik/0000-0002-8052-0107; Urbina, Hector/0000-0002-5570-4537; DEL PRADO, RUTH/0000-0002-7497-2510; Wang, Yan/0000-0002-5950-8904; Zamora, Juan Carlos/0000-0002-9243-2999; Ruibal Villasenor, Constantino/0000-0003-3494-7051; May, Tom/0000-0003-2214-4972; McTaggart, Alistair/0000-0002-0996-1313; Raja, Huzefa/0000-0002-0824-9463; Yurkov, Andrey/0000-0002-1072-5166; CARDINALI, GIANLUIGI/0000-0002-4522-7925; McTaggart, Alistair/0000-0002-3681-7632; CAI, LEI/0000-0002-8131-7274; Hagen, Ferry/0000-0002-5622-1916; Meyer, Wieland/0000-0001-9933-8340; Telleria, M. Teresa/0000-0002-9876-6914; Duenas, Margarita/0000-0003-0621-8003; Crous, Pedro/0000-0001-9085-8825; Weir, Bevan/0000-0003-2580-0701; Duong, Tuan/0000-0001-5110-1854; Lumbsch, Thorsten/0000-0003-1512-835X; Zhang, Ning/0000-0003-0755-2505; Lombard, Lorenzo/0000-0002-5942-5375; WeiSS, Michael/0000-0002-4869-9186; Groenewald, Marizeth/0000-0003-0835-5925; de Beer, Z. Wilhelm/0000-0001-9758-8987; Garcia, Miguel Angel/0000-0002-0366-043X; Martin, Maria /0000-0002-1235-4418; Divakar, Pradeep/0000-0002-0300-0124; Hollingsworth, Peter/0000-0003-0602-0654 FU Alfred P. Sloan Foundation; National Library of Medicine at the National Institutes of Health; Life Technologies Corporation FX We thank David L. Hawksworth, Martin Bidartondo, and numerous other colleagues for critical comments. This work was organized under the Fungal Working Group of the Consortium for the Barcode of Life (CBOL), which provided support from its funding from the Alfred P. Sloan Foundation. Support was also provided by the Intramural Research Program of the National Library of Medicine at the National Institutes of Health, Life Technologies Corporation, and the individual funders to authors who provided sequences for our analysis. Publication charges were provided by the International Barcode of Life Network from Genome Canada through the Ontario Genomics Institute. NR 83 TC 1074 Z9 1147 U1 65 U2 607 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6241 EP 6246 DI 10.1073/pnas.1117018109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100068 PM 22454494 ER PT J AU Lovatt, D Xu, QW Liu, W Takano, T Smith, NA Schnermann, J Tieu, K Nedergaard, M AF Lovatt, Ditte Xu, Qiwu Liu, Wei Takano, Takahiro Smith, Nathan A. Schnermann, Jurgen Tieu, Kim Nedergaard, Maiken TI Neuronal adenosine release, and not astrocytic ATP release, mediates feedback inhibition of excitatory activity SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE purinergic signaling; purine; glia; calcium signaling ID NUCLEOSIDE TRANSPORTERS; SYNAPTIC-TRANSMISSION; DEPENDENT RELEASE; INDUCED SEIZURES; NERVOUS-SYSTEM; A(1) RECEPTOR; BRAIN; HIPPOCAMPUS; ACTIVATION; STIMULATION AB Adenosine is a potent anticonvulsant acting on excitatory synapses through A1 receptors. Cellular release of ATP, and its subsequent extracellular enzymatic degradation to adenosine, could provide a powerful mechanism for astrocytes to control the activity of neural networks during high-intensity activity. Despite adenosine's importance, the cellular source of adenosine remains unclear. We report here that multiple enzymes degrade extracellular ATP in brain tissue, whereas only Nt5e degrades AMP to adenosine. However, endogenous A1 receptor activation during cortical seizures in vivo or heterosynaptic depression in situ is independent of Nt5e activity, and activation of astrocytic ATP release via Ca2+ photolysis does not trigger synaptic depression. In contrast, selective activation of postsynaptic CA1 neurons leads to release of adenosine and synaptic depression. This study shows that adenosine-mediated synaptic depression is not a consequence of astrocytic ATP release, but is instead an autonomic feedback mechanism that suppresses excitatory transmission during prolonged activity. C1 [Lovatt, Ditte; Xu, Qiwu; Liu, Wei; Takano, Takahiro; Smith, Nathan A.; Tieu, Kim; Nedergaard, Maiken] Univ Rochester, Med Ctr, Ctr Translat Neuromed, Dept Neurosurg, Rochester, NY 14642 USA. [Lovatt, Ditte; Xu, Qiwu; Liu, Wei; Takano, Takahiro; Smith, Nathan A.; Tieu, Kim; Nedergaard, Maiken] Univ Rochester, Med Ctr, Ctr Translat Neuromed, Dept Neurol, Rochester, NY 14642 USA. [Schnermann, Jurgen] NIDDK, NIH, Bethesda, MD 20892 USA. RP Lovatt, D (reprint author), Univ Rochester, Med Ctr, Ctr Translat Neuromed, Dept Neurosurg, Rochester, NY 14642 USA. EM lovatt@upenn.edu; nedergaard@urmc.rochester.edu RI Tieu, Kim/F-5426-2012 OI Tieu, Kim/0000-0001-6606-4371 FU G. Harold and Leila Y. Mathers Charitable Foundation; Adelson Medical Research Foundation; National Institutes of Health [NS075177, NS078304, F31NS073390] FX We thank Herbert Zimmermann, Marisa Cotrina, and Takumi Fujita for valuable suggestions to this manuscript. This work was supported by the G. Harold and Leila Y. Mathers Charitable Foundation, the Adelson Medical Research Foundation, and the National Institutes of Health. Grants NS075177 and NS078304 (to M.N.) and F31NS073390 (to N.A.S.). NR 49 TC 111 Z9 111 U1 2 U2 14 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6265 EP 6270 DI 10.1073/pnas.1120997109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100072 PM 22421436 ER PT J AU Rissman, RA Staup, MA Lee, AR Justice, NJ Rice, KC Vale, W Sawchenko, PE AF Rissman, Robert A. Staup, Michael A. Lee, Allyson Roe Justice, Nicholas J. Rice, Kenner C. Vale, Wylie Sawchenko, Paul E. TI Corticotropin-releasing factor receptor-dependent effects of repeated stress on tau phosphorylation, solubility, and aggregation SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE neurofibrillary tangles; western blot; pathology; electron microscopy ID PAIRED HELICAL FILAMENTS; TRANSGENIC MOUSE MODEL; ALZHEIMERS-DISEASE; IN-VIVO; COGNITIVE DEFICITS; CRF RECEPTORS; MICE; TAUOPATHY; PATHOLOGY; SYSTEM AB Exposure and/or sensitivity to stress have been implicated as conferring risk for development of Alzheimer's disease (AD). Although the basis for such a link remains unclear, we previously reported differential involvement of corticotropin-releasing factor receptor (CRFR) 1 and 2 in acute stress-induced tau phosphorylation (tau-P) and solubility in the hippocampus. Here we examined the role of CRFRs in tau-P induced by repeated stress and the structural manifestations of altered tau solubility. Robust tau-P responses were seen in WT and CRFR2 null mice exposed to repeated stress, which were sustained at even 24 h after the final stress exposure. A portion of phosphorylated tau in these mice was sequestered in detergent-soluble cellular fractions. In contrast, CRFR1 and CRFR double-KO mice did not exhibit repeated stress-induced alterations in tau-P or solubility. Similarly, treatment with CRFR1 antagonist attenuated repeated stress-induced tau-P. Using histochemical approaches in a transgenic CRFR1 reporter mouse line, we found substantial overlap between hippocampal CRFR1 expression and cells positive for phosphorylated tau after exposure to repeated stress. Ultrastructural analysis of negatively stained extracts from WT and CRFR2 null mice identified globular aggregates that displayed positive immunogold labeling for tau-P, as well as conformational changes in tau (MC1) seen in early AD. Given that repeated stress exposure results in chronic increases in hippocampal tau-P and its sequestration in an insoluble (and potentially prepathogenic) form, our data may define a link between stress and an AD-related pathogenic mechanism. C1 [Rissman, Robert A.; Staup, Michael A.; Lee, Allyson Roe] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA. [Justice, Nicholas J.] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Rice, Kenner C.] Natl Inst Drug Abuse & Alcohol Abuse & Alcoholism, Chem Biol Res Branch, NIH, Bethesda, MD 20892 USA. [Vale, Wylie] Salk Inst Biol Studies, Peptide Biol Lab, La Jolla, CA 92037 USA. [Sawchenko, Paul E.] Salk Inst Biol Studies, Neuronal Struct & Funct Lab, La Jolla, CA 92037 USA. RP Rissman, RA (reprint author), Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA. EM rrissman@ucsd.edu FU National Institutes of Health [AG032755, DK026741, AG010483]; Alzheimer's Art Quilt Initiative; Alzheimer's Association; Foundation for Medical Research; Shiley-Marcos Alzheimer's Disease Research Center at University of California at San Diego [AG005131] FX We dedicate this paper to the memory of Dr. Wylie Vale, long time mentor and friend, whose leadership in the area of CRF and stress biology made this work possible, and whose colleagueship made it a joy. We thank Drs. S. Wagner and E. Masliah (University of California at San Diego) for helpful discussions and Dr. P. Davies for generously providing tau antibodies MC1 and PHF-1. This work was supported by National Institutes of Health Grants AG032755, DK026741, and AG010483; the Alzheimer's Art Quilt Initiative; the Alzheimer's Association; the Foundation for Medical Research; and the Shiley-Marcos Alzheimer's Disease Research Center at University of California at San Diego through Pilot Grant AG005131. W. V. was a senior investigator at the Foundation for Medical Research, and P. E. S. is a senior investigator at the Foundation for Medical Research. NR 54 TC 39 Z9 40 U1 0 U2 15 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 17 PY 2012 VL 109 IS 16 BP 6277 EP 6282 DI 10.1073/pnas.1203140109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 931UZ UT WOS:000303246100074 PM 22451915 ER PT J AU Rogozin, IB Carmel, L Csuros, M Koonin, EV AF Rogozin, Igor B. Carmel, Liran Csuros, Miklos Koonin, Eugene V. TI Origin and evolution of spliceosomal introns SO BIOLOGY DIRECT LA English DT Review DE Intron sliding; Intron gain; Intron loss; Spliceosome; Splicing signals; Evolution of exon/intron structure; Alternative splicing; Phylogenetic trees; Mobile domains; Eukaryotic ancestor ID MESSENGER-RNA EXPORT; HIGHLY EXPRESSED GENES; CHORDATE OIKOPLEURA-DIOICA; EXONIC SPLICING ENHANCERS; HUMAN HOUSEKEEPING GENES; CONSERVED NONCODING DNA; RIBOSOMAL-PROTEIN GENES; EUKARYOTIC EVOLUTION; MOLECULAR EVOLUTION; SELECTIVE CONSTRAINTS AB Evolution of exon-intron structure of eukaryotic genes has been a matter of long-standing, intensive debate. The introns early concept, later rebranded 'introns first' held that protein-coding genes were interrupted by numerous introns even at the earliest stages of life's evolution and that introns played a major role in the origin of proteins by facilitating recombination of sequences coding for small protein/peptide modules. The introns-late concept held that introns emerged only in eukaryotes and new introns have been accumulating continuously throughout eukaryotic evolution. Analysis of orthologous genes from completely sequenced eukaryotic genomes revealed numerous shared intron positions in orthologous genes from animals and plants and even between animals, plants and protists, suggesting that many ancestral introns have persisted since the last eukaryotic common ancestor (LECA). Reconstructions of intron gain and loss using the growing collection of genomes of diverse eukaryotes and increasingly advanced probabilistic models convincingly show that the LECA and the ancestors of each eukaryotic supergroup had intron-rich genes, with intron densities comparable to those in the most intron-rich modern genomes such as those of vertebrates. The subsequent evolution in most lineages of eukaryotes involved primarily loss of introns, with only a few episodes of substantial intron gain that might have accompanied major evolutionary innovations such as the origin of metazoa. The original invasion of self-splicing Group II introns, presumably originating from the mitochondrial endosymbiont, into the genome of the emerging eukaryote might have been a key factor of eukaryogenesis that in particular triggered the origin of endomembranes and the nucleus. Conversely, splicing errors gave rise to alternative splicing, a major contribution to the biological complexity of multicellular eukaryotes. There is no indication that any prokaryote has ever possessed a spliceosome or introns in protein-coding genes, other than relatively rare mobile self-splicing introns. Thus, the introns-first scenario is not supported by any evidence but exon-intron structure of protein-coding genes appears to have evolved concomitantly with the eukaryotic cell, and introns were a major factor of evolution throughout the history of eukaryotes. This article was reviewed by I. King Jordan, Manuel Irimia (nominated by Anthony Poole), Tobias Mourier (nominated by Anthony Poole), and Fyodor Kondrashov. For the complete reports, see the Reviewers' Reports section. C1 [Rogozin, Igor B.; Koonin, Eugene V.] Natl Ctr Biotechnol Informat NLM NIH, Bethesda, MD 20894 USA. [Carmel, Liran] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel. [Csuros, Miklos] Univ Montreal, Dept Comp Sci & Operat Res, Montreal, PQ, Canada. RP Koonin, EV (reprint author), Natl Ctr Biotechnol Informat NLM NIH, 8600 Rockville Pike,Bldg 38A, Bethesda, MD 20894 USA. EM koonin@ncbi.nlm.nih.gov FU National Library of Medicine at National Institutes of Health/DHHS (NIH) [Z01LM000073-12]; National Sciences and Engineering Research Council of Canada; European Union [PIRG05-GA-2009-248639] FX We thank Ben Busby, Nicholas Dibb, Cedric Feschotte, Michael Lynch, Masatoshi Nei, Scott Roy, Arlin Stoltzfus, and Yuri Wolf for useful discussions. This work was supported in part by the Intramural Research Program of the National Library of Medicine at National Institutes of Health/DHHS (the NIH grant Z01LM000073-12), by a research grant from the National Sciences and Engineering Research Council of Canada, and by the European Union Marie Curie International Reintegration Grant (PIRG05-GA-2009-248639). NR 287 TC 81 Z9 82 U1 10 U2 116 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1745-6150 J9 BIOL DIRECT JI Biol. Direct PD APR 16 PY 2012 VL 7 AR 11 DI 10.1186/1745-6150-7-11 PG 28 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 031EP UT WOS:000310622100001 PM 22507701 ER PT J AU Driscoll, I Martin, B An, Y Maudsley, S Ferrucci, L Mattson, MP Resnick, SM AF Driscoll, Ira Martin, Bronwen An, Yang Maudsley, Stuart Ferrucci, Luigi Mattson, Mark P. Resnick, Susan M. TI Plasma BDNF Is Associated with Age-Related White Matter Atrophy but Not with Cognitive Function in Older, Non-Demented Adults SO PLOS ONE LA English DT Article ID FACTOR VAL66MET POLYMORPHISM; NEUROTROPHIC FACTOR; HIPPOCAMPAL VOLUME; ENERGY RESTRICTION; IMPAIRMENT; HEALTHY; DISEASE; WEIGHT; IMPACT; WOMEN AB Brain derived neurotrophic factor (BDNF) seems to be involved in regulation of synaptic plasticity and neurogenesis. BDNF plasma and serum levels have been associated with depression, Alzheimer's disease, and other psychiatric and neurodegenerative disorders. In a community sample, drawn from the Baltimore Longitudinal Study of Aging (BLSA), we examined whether BDNF plasma concentration was associated with rates of age-related change in cognitive performance (n = 429) and regional brain volume (n = 59). Plasma BDNF levels, which were significantly higher in females (p<0.05), were not associated with either concurrent cognitive performance or rates of age-related change in performance across cognitive domains (p's>0.05). Sex differences in the relationship between BDNF and the trajectories of regional brain volume changes were observed for the whole brain and frontal white matter volumes (p<0.05), whereby lower plasma BDNF was associated with steeper volume decline in females but not males. Together, our findings contribute to furthering the understanding of the relationships between plasma BDNF, structural brain integrity and cognition. Potential mechanisms mediating these relationships merit further investigation. C1 [Driscoll, Ira; An, Yang; Resnick, Susan M.] NIA, Lab Behav Neurosci, Baltimore, MD 21224 USA. [Driscoll, Ira] Univ Wisconsin Milwaukee, Dept Psychol, Milwaukee, WI USA. [Martin, Bronwen] NIA, Clin Invest Lab, Baltimore, MD 21224 USA. [Maudsley, Stuart; Mattson, Mark P.] NIA, Neurosci Lab, Baltimore, MD 21224 USA. [Ferrucci, Luigi] NIA, Clin Res Branch, Baltimore, MD 21224 USA. RP Driscoll, I (reprint author), NIA, Lab Behav Neurosci, Baltimore, MD 21224 USA. EM driscoli@uwm.edu FU Intramural Research Program of the National Institute on Aging FX This research was supported by the Intramural Research Program of the National Institute on Aging. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 30 Z9 30 U1 0 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 16 PY 2012 VL 7 IS 4 AR e35217 DI 10.1371/journal.pone.0035217 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VO UT WOS:000305345000049 PM 22523577 ER PT J AU Anwar, K Klemm, RW Condon, A Severin, KN Zhang, M Ghirlando, R Hu, JJ Rapoport, TA Prinz, WA AF Anwar, Kamran Klemm, Robin W. Condon, Amanda Severin, Katharina N. Zhang, Miao Ghirlando, Rodolfo Hu, Junjie Rapoport, Tom A. Prinz, William A. TI The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae SO JOURNAL OF CELL BIOLOGY LA English DT Article ID ENDOPLASMIC-RETICULUM MEMBRANES; SEDIMENTATION-VELOCITY; NUCLEAR-FUSION; ANALYTICAL ULTRACENTRIFUGATION; SACCHAROMYCES-CEREVISIAE; GOLGI-APPARATUS; YEAST; ARABIDOPSIS; ATLASTIN; REVEALS AB The endoplasmic reticulum (ER) forms a network of tubules and sheets that requires homotypic membrane fusion to be maintained. In metazoans, this process is mediated by dynamin-like guanosine triphosphatases (GTPases) called atlastins (ATLs), which are also required to maintain ER morphology. Previous work suggested that the dynamin-like GTPase Sey1p was needed to maintain ER morphology in Saccharomyces cerevisiae. In this paper, we demonstrate that Sey1p, like ATLs, mediates homotypic ER fusion. The absence of Sey1p resulted in the ER undergoing delayed fusion in vivo and proteoliposomes containing purified Sey1p fused in a GTP-dependent manner in vitro. Sey1p could be partially replaced by ATL1 in vivo. Like ATL1, Sey1p underwent GTP-dependent dimerization. We found that the residual ER-ER fusion that occurred in cells lacking Sey1p required the ER SNARE Ufe1p. Collectively, our results show that Sey1p and its homologues function analogously to ATLs in mediating ER fusion. They also indicate that S. cerevisiae has an alternative fusion mechanism that requires ER SNAREs. C1 [Anwar, Kamran; Condon, Amanda; Prinz, William A.] NIDDK, Lab Mol & Cell Biol, NIH, Bethesda, MD 20892 USA. [Ghirlando, Rodolfo] NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. [Klemm, Robin W.; Severin, Katharina N.; Rapoport, Tom A.] Harvard Univ, Sch Med, Dept Cell Biol, Howard Hughes Med Inst, Boston, MA 02115 USA. [Zhang, Miao; Hu, Junjie] Nankai Univ, Coll Life Sci, Dept Genet & Cell Biol, Tianjin 300071, Peoples R China. [Zhang, Miao; Hu, Junjie] Nankai Univ, Coll Life Sci, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China. RP Prinz, WA (reprint author), NIDDK, Lab Mol & Cell Biol, NIH, Bethesda, MD 20892 USA. EM wprinz@helix.nih.gov RI Hu, Junjie/F-9713-2013; Klemm, Robin/C-5463-2014 OI Klemm, Robin/0000-0003-2313-8240 FU National Basic Research Program of China (973 Program) [2010CB833702]; National Institute of Diabetes and Digestive and Kidney Diseases FX J. Hu is supported by the National Basic Research Program of China (973 Program; grant 2010CB833702). W.A. Prinz, K. Anwar, and A. Condon are supported by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases. T.A. Rapoport is a Howard Hughes Medical Institute Investigator. NR 27 TC 42 Z9 42 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD APR 16 PY 2012 VL 197 IS 2 BP 209 EP 217 DI 10.1083/jcb.201111115 PG 9 WC Cell Biology SC Cell Biology GA 927MU UT WOS:000302912600007 PM 22508509 ER PT J AU Kapetanovic, IM Lyubimov, AV Kabirova, EV Kabirov, KK Rasay, L Swezey, R Green, C Kopelovich, L AF Kapetanovic, Izet M. Lyubimov, Alexander V. Kabirova, Elena V. Kabirov, Kasim K. Rasay, Laura Swezey, Robert Green, Carol Kopelovich, Levy TI Effects of bacterial and presystemic nitroreductase metabolism of 2-chloro-5-nitro-N-phenylbenzamide on its mutagenicity and bioavailability SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE Nitroreductases; Ames assay; Nitro compounds; Rat; Dog; Chemoprevention ID DRUGS AB 2-Chloro-5-nitro-N-phenylbenzamide (GW9662), a potent irreversible PPAR-gamma antagonist, has shown promise as a cancer chemopreventive agent and is undergoing preclinical evaluations. Studies were initiated to assess its bacterial mutagenicity and pharmacokinetic profile in two animal species prior to subchronic oral toxicity evaluations and the results are reported here. GW9662 was mutagenic in both TA98 and TA100 bacterial strains with and without metabolic activation but was negative in the nitroreductase-deficient strains (TA98NR and TA100NR) also with and without metabolic activation, indicating that GW9662 mutagenicity is dependent on nitroreduction. The mutagenic activity was predominantly via a base-substitution mechanism. Following oral dosing in rats and dogs, the parent compound, GW9662, was virtually absent from plasma samples, but there was chromatographic evidence for the presence of metabolites in the plasma as a result of oral dosing. Metabolite identification studies showed that an amine metabolite ACPB (5-amino-2-chloro-N-phenylbenzamide), a product of nitro reduction, was the predominant species exhibiting large and persistent plasma levels. Thus systemic circulation of GW9662 has been attained largely in the form of its reduced metabolite, probably a product of gut bacterial metabolism. GW9662 was detectable in plasma of rats and dogs after intravenous dose albeit at low concentrations. Pharmacokinetic analysis following intravenous dosing in rats showed a rapid clearance and an extensive tissue distribution which could have accounted for the very low plasma levels. Of note, the amine metabolite was absent following intravenous dosing in both rats and dogs, confirming it being a product of presystemic metabolism. The potential utility of GW9662 as a chemopreventive agent, especially as an Estrogen Receptor-alpha (ER-alpha) inducer in an otherwise ER-a negative breast tissue, is of great interest. However, the results shown here suggest that additional animal toxicological and bioavailability studies are required to establish a role of GW9662 as a chemopreventive agent. Published by Elsevier Ireland Ltd. C1 [Kapetanovic, Izet M.] NCI, Chemoprevent Agent Dev Res Grp, Canc Prevent Div, Bethesda, MD 20892 USA. [Lyubimov, Alexander V.; Kabirova, Elena V.; Kabirov, Kasim K.] Univ Illinois, Toxicol Res Lab, Chicago, IL 60612 USA. [Rasay, Laura; Swezey, Robert; Green, Carol] SRI Int, Biosci Div, Menlo Pk, CA 94025 USA. RP Kapetanovic, IM (reprint author), NCI, Chemoprevent Agent Dev Res Grp, Canc Prevent Div, 6130 Execut Blvd,Rm 2116, Bethesda, MD 20892 USA. EM kapetani@mail.nih.gov FU National Cancer Institute, Department of Health and Human Services [N01-CN-43305, N01-CN-43306] FX These studies were supported by contract numbers N01-CN-43305 and N01-CN-43306 from the National Cancer Institute, Department of Health and Human Services. NR 5 TC 1 Z9 1 U1 0 U2 8 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-2797 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PD APR 15 PY 2012 VL 197 IS 1 BP 16 EP 22 DI 10.1016/j.cbi.2012.03.002 PG 7 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA 941OP UT WOS:000303972800003 PM 22450444 ER PT J AU Canepa, M Strait, JB Abramov, D Milaneschi, Y AlCihatrif, M Moni, M Ramachandran, R Najjar, SS Brunelli, C Abraham, TP Lakatta, EG Ferrucci, L AF Canepa, Marco Strait, James B. Abramov, Dmitry Milaneschi, Yuri AlCihatrif, Majd Moni, Monika Ramachandran, Ramona Najjar, Samer S. Brunelli, Claudio Abraham, Theodore P. Lakatta, Edward G. Ferrucci, Luigi TI Contribution of Central Adiposity to Left Ventricular Diastolic Function (from the Baltimore Longitudinal Study of Aging) SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID OF-THE-LITERATURE; BODY-MASS INDEX; TO-HIP RATIO; HEART-FAILURE; WAIST CIRCUMFERENCE; CARDIOVASCULAR-DISEASE; EJECTION FRACTION; CENTRAL OBESITY; RISK; DYSFUNCTION AB We examined the relations of central adiposity with left ventricular (LV) diastolic dysfunction in men and women who participated in the Baltimore Longitudinal Study of Aging, a prospective community-based study of older persons. The sample for this cross-sectional analysis included 399 women and 370 men. Central adiposity was estimated using the waist circumference (WC) and global adiposity using the body mass index (BMI). Using data from a comprehensive echocardiographic study that included tissue Doppler imaging, diastolic function was graded according to 3 parameters (E/A ratio, E/Em ratio, and left atrial volume index). In the logistic regression models adjusted for age, gender, cardiovascular risk factors, and hemodynamic parameters, WC and BMI were both independently associated with LV diastolic dysfunction. However, when both WC and BMI were in the same model, only WC remained significantly associated with LV diastolic dysfunction (odds ratio 1.04, 95% confidence interval 1.01 to 1.08, p = 0.02). In the gender-stratified analyses, WC was significantly associated with LV diastolic dysfunction independently of BMI-in women (odds ratio 1.08, 95% confidence interval 1.04 to 1.14, p < 0.001) but not in men (odds ratio 1.00, 95% confidence interval 0.95 to 1.05, p = 0.91). Additional adjustment for LV mass index failed to modify these relations. In conclusion, the adverse effect of central adiposity on LV diastolic function was independent of general adiposity and more pronounced among women. The effect of visceral adiposity on LV diastolic dysfunction would benefit from confirmation in longitudinal studies. Published by Elsevier Inc. (Am J Cardiol 2012;109:1171-1178) C1 [Canepa, Marco; Strait, James B.; Milaneschi, Yuri; Ramachandran, Ramona; Ferrucci, Luigi] NIA, Longitudinal Studies Sect, Clin Res Branch, NIH, Baltimore, MD 21224 USA. [Canepa, Marco; Strait, James B.; Abramov, Dmitry; AlCihatrif, Majd; Moni, Monika; Najjar, Samer S.; Lakatta, Edward G.] NIA, Cardiovasc Sci Lab, Human Cardiovasc Studies Unit, NIH, Baltimore, MD 21224 USA. [Najjar, Samer S.] Washington Hosp Ctr, Res Inst, Washington, DC 20010 USA. [Canepa, Marco; Brunelli, Claudio] Univ Genoa, Div Cardiol, Res Ctr Cardiovasc Biol, Genoa, Italy. [Abraham, Theodore P.] Johns Hopkins Univ, Div Cardiovasc Dis, Baltimore, MD USA. RP Canepa, M (reprint author), NIA, Longitudinal Studies Sect, Clin Res Branch, NIH, Baltimore, MD 21224 USA. EM marco.canepa@nih.gov FU National Institutes of Health, National Institute on Aging (Bethesda, Maryland) FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Institute on Aging (Bethesda, Maryland). NR 30 TC 22 Z9 23 U1 0 U2 2 PU EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC PI BRIDGEWATER PA 685 ROUTE 202-206 STE 3, BRIDGEWATER, NJ 08807 USA SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD APR 15 PY 2012 VL 109 IS 8 BP 1171 EP 1178 DI 10.1016/j.amjcard.2011.11.054 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 931NP UT WOS:000303226900015 PM 22257709 ER PT J AU Hutter, CM Chang-Claude, J Slattery, ML Pflugeisen, BM Lin, Y Duggan, D Nan, HM Lemire, M Rangrej, J Figueiredo, JC Jiao, S Harrison, TA Liu, Y Chen, LS Stelling, DL Warnick, GS Hoffmeister, M Kury, S Fuchs, CS Giovannucci, E Hazra, A Kraft, P Hunter, DJ Gallinger, S Zanke, BW Brenner, H Frank, B Ma, J Ulrich, CM White, E Newcomb, PA Kooperberg, C LaCroix, AZ Prentice, RL Jackson, RD Schoen, RE Chanock, SJ Berndt, SI Hayes, RB Caan, BJ Potter, JD Hsu, L Bezieau, S Chan, AT Hudson, TJ Peters, U AF Hutter, Carolyn M. Chang-Claude, Jenny Slattery, Martha L. Pflugeisen, Bethann M. Lin, Yi Duggan, David Nan, Hongmei Lemire, Mathieu Rangrej, Jagadish Figueiredo, Jane C. Jiao, Shuo Harrison, Tabitha A. Liu, Yan Chen, Lin S. Stelling, Deanna L. Warnick, Greg S. Hoffmeister, Michael Kuery, Sebastien Fuchs, Charles S. Giovannucci, Edward Hazra, Aditi Kraft, Peter Hunter, David J. Gallinger, Steven Zanke, Brent W. Brenner, Hermann Frank, Bernd Ma, Jing Ulrich, Cornelia M. White, Emily Newcomb, Polly A. Kooperberg, Charles LaCroix, Andrea Z. Prentice, Ross L. Jackson, Rebecca D. Schoen, Robert E. Chanock, Stephen J. Berndt, Sonja I. Hayes, Richard B. Caan, Bette J. Potter, John D. Hsu, Li Bezieau, Stephane Chan, Andrew T. Hudson, Thomas J. Peters, Ulrike TI Characterization of Gene-Environment Interactions for Colorectal Cancer Susceptibility Loci SO CANCER RESEARCH LA English DT Article ID GENOME-WIDE ASSOCIATION; COLON-CANCER; CHROMOSOME 8Q24; LIFE-STYLE; RISK LOCI; METAANALYSIS; HEALTH; VARIANTS; FRUIT; SCAN AB Genome-wide association studies (GWAS) have identified more than a dozen loci associated with colorectal cancer (CRC) risk. Here, we examined potential effect-modification between single-nucleotide polymorphisms (SNP) at 10 of these loci and probable or established environmental risk factors for CRC in 7,016 CRC cases and 9,723 controls from nine cohort and case-control studies. We used meta-analysis of an efficient empirical-Bayes estimator to detect potential multiplicative interactions between each of the SNPs [rs16892766 at8q23.3 (EIF3H/UTP23), rs6983267 at 8q24 (MYC), rs10795668 at 10p14 (FLJ3802842), rs3802842 at 11q23 (LOC120376), rs4444235 at 14q22.2 (BMP4), rs4779584 at 15q13 (GREM1), rs9929218 at 16q22.1 (CDH1), rs4939827 at 18q21 (SMAD7), rs10411210 at 19q13.1 (RHPN2), and rs961253 at 20p12.3 (BMP2)] and select major CRC risk factors (sex, body mass index, height, smoking status, aspirin/nonsteroidal anti-inflammatory drug use, alcohol use, and dietary intake of calcium, folate, red meat, processed meat, vegetables, fruit, and fiber). The strongest statistical evidence for a gene-environment interaction across studies was for vegetable consumption and rs16892766, located on chromosome 8q23.3, near the EIF3H and UTP23 genes (nominal P-interaction = 1.3 x 10(-4); adjusted P = 0.02). The magnitude of the main effect of the SNP increased with increasing levels of vegetable consumption. No other interactions were statistically significant after adjusting for multiple comparisons. Overall, the association of most CRC susceptibility loci identified in initial GWAS seems to be invariant to the other risk factors considered; however, our results suggest potential modification of the rs16892766 effect by vegetable consumption. Cancer Res; 72(8); 2036-44. (C) 2012 AACR. C1 [Hutter, Carolyn M.; Pflugeisen, Bethann M.; Lin, Yi; Jiao, Shuo; Harrison, Tabitha A.; Stelling, Deanna L.; Warnick, Greg S.; Ulrich, Cornelia M.; White, Emily; Newcomb, Polly A.; Kooperberg, Charles; LaCroix, Andrea Z.; Prentice, Ross L.; Potter, John D.; Hsu, Li; Peters, Ulrike] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Hutter, Carolyn M.; Ulrich, Cornelia M.; White, Emily; Newcomb, Polly A.; Potter, John D.; Peters, Ulrike] Univ Washington, Sch Publ Hlth, Dept Epidemiol, Seattle, WA 98195 USA. [Kooperberg, Charles; Hsu, Li] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Chang-Claude, Jenny] German Canc Res Ctr, Div Canc Epidemiol, D-6900 Heidelberg, Germany. [Hoffmeister, Michael; Brenner, Hermann; Frank, Bernd] German Canc Res Ctr, Div Clin Epidemiol & Aging Res, D-6900 Heidelberg, Germany. [Ulrich, Cornelia M.] German Canc Res Ctr, Div Prevent Oncol, D-6900 Heidelberg, Germany. [Slattery, Martha L.] Univ Utah, Dept Internal Med, Hlth Sci Ctr, Salt Lake City, UT 84112 USA. [Duggan, David] Translat Genom Res Inst, Phoenix, AZ USA. [Nan, Hongmei; Fuchs, Charles S.; Giovannucci, Edward; Hazra, Aditi; Ma, Jing; Chan, Andrew T.] Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA. [Nan, Hongmei; Fuchs, Charles S.; Giovannucci, Edward; Hazra, Aditi; Ma, Jing; Chan, Andrew T.] Harvard Univ, Sch Med, Boston, MA USA. [Nan, Hongmei; Hazra, Aditi; Kraft, Peter; Hunter, David J.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Program Mol & Genet Epidemiol, Boston, MA 02115 USA. [Giovannucci, Edward] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Fuchs, Charles S.] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA. [Chan, Andrew T.] Massachusetts Gen Hosp, Div Gastroenterol, Boston, MA 02114 USA. [Lemire, Mathieu; Rangrej, Jagadish; Hudson, Thomas J.] Ontario Inst Canc Res, Toronto, ON, Canada. [Gallinger, Steven] Toronto Gen Hosp, Univ Hlth Network, Dept Surg, Toronto, ON, Canada. [Hudson, Thomas J.] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada. [Hudson, Thomas J.] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada. [Figueiredo, Jane C.] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. [Liu, Yan] Stephens & Associates, Carrollton, TX USA. [Chen, Lin S.] Univ Chicago, Dept Hlth Studies, Chicago, IL 60637 USA. [Kuery, Sebastien; Bezieau, Stephane] CHU Nantes, Serv Genet Med, F-44035 Nantes 01, France. [Zanke, Brent W.] Ottawa Hosp Res Inst, Clin Epidemiol Program, Ottawa, ON, Canada. [Jackson, Rebecca D.] Ohio State Univ, Div Endocrinol Diabet & Metab, Columbus, OH 43210 USA. [Schoen, Robert E.] Univ Pittsburgh, Dept Epidemiol, Med Ctr, Pittsburgh, PA 15261 USA. [Chanock, Stephen J.; Berndt, Sonja I.] NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Hayes, Richard B.] NYU, Sch Med, Dept Environm Med, Div Epidemiol, New York, NY USA. [Caan, Bette J.] Kaiser Permanente Med Care Program, Div Res, Oakland, CA 94611 USA. [Potter, John D.] Massey Univ, Ctr Publ Hlth Res, Wellington, New Zealand. RP Hutter, CM (reprint author), 1100 Fairview Ave N,M4-B402,POB 19024, Seattle, WA 98109 USA. EM chutter@fhcrc.org RI Gallinger, Steven/E-4575-2013; Hoffmeister, Michael/B-5745-2012; KURY, Sebastien/G-5971-2015; Bezieau, stephane/G-5621-2015; Brenner, Hermann/B-4627-2017; OI Hayes, Richard/0000-0002-0918-661X; Hoffmeister, Michael/0000-0002-8307-3197; KURY, Sebastien/0000-0001-5497-0465; Bezieau, stephane/0000-0003-0095-1319; Brenner, Hermann/0000-0002-6129-1572; Potter, John/0000-0001-5439-1500 FU Ontario Research Fund; Canadian Institutes of Health Research; Canadian Cancer Society Research Institute; Ontario Institute for Cancer Research through Ontario Ministry of Research; regional Hospital Clinical Research Program (PHRC); Regional Council of Pays de la Loire; Groupement des Entreprises Francaises dans la Lutte contre le Cancer (GEFLUC); Association Anne de Bretagne Genetique; Ligue Regionale Contre le Cancer (LRCC); National Cancer Institute, NIH [CA-95-011]; German Research Council (Deutsche Forschungsgemeinschaft) [BR 1704/6-1, BR 1704/6-3, BR 1704/6-4, CH 117/1-1]; German Federal Ministry of Education and Research [01KH0404, 01ER0814]; National Cancer Institute, NIH, U.S. Department of Health and Human Services [R01 CA48998]; National Cancer Institute; National Institutes of Health; U.S. Department of Health and Human Services [R01 CA059045, R25 CA094880, U01 CA137088]; Institutes of Health; NIH [P01 CA 055075, P50 CA 127003, R01 137178, P01 CA 087969]; Division of Cancer Epidemiology and Genetics; Division of Cancer Prevention; NIH, Genes, Environment and Health Initiative [NIH GEI] [Z01 CP 010200, U01 HG 004438]; Gene Environment Association Studies, GENEVA Coordinating Center [U01 HG004446]; Johns Hopkins University Center for Inherited Disease Research; National Heart, Lung, and Blood Institute, NIH; U. S. Department of Health and Human Services [HHSN268201100001C-4C, HHSN268201100046C, HHSN271201100004C, NO1WH4421]; [U01 CA074783] FX ARTIC was supported by a GL2 grant from the Ontario Research Fund, the Canadian Institutes of Health Research, and the Cancer Risk Evaluation (CaRE) Program grant from the Canadian Cancer Society Research Institute. T.J. Hudson and B. W. Zanke are recipients of Senior Investigator Awards from the Ontario Institute for Cancer Research, through generous support from the Ontario Ministry of Research.; ASTERISK was supported by a regional Hospital Clinical Research Program (PHRC) and supported by the Regional Council of Pays de la Loire, the Groupement des Entreprises Francaises dans la Lutte contre le Cancer (GEFLUC), the Association Anne de Bretagne Genetique, and the Ligue Regionale Contre le Cancer (LRCC).; CCFR was supported by the National Cancer Institute, NIH under RFA # CA-95-011 and through cooperative agreements with members of the Colon Cancer Family Registry and P.I.s. The Colon CFR Center, Ontario Registry for Studies of Familial CRC, contributed data to this manuscript and was supported by (U01 CA074783).; DACHS was supported by grants from the German Research Council (Deutsche Forschungsgemeinschaft, BR 1704/6-1, BR 1704/6-3, BR 1704/6-4, and CH 117/1-1), and the German Federal Ministry of Education and Research (01KH0404 and 01ER0814).; DALS was supported by the National Cancer Institute, NIH, U.S. Department of Health and Human Services (R01 CA48998 to M.L. Slattery).; Funding for the genome-wide scan of DALS, PLCO, and WHI was provided by the National Cancer Institute, National Institutes of Health, U.S. Department of Health and Human Services (R01 CA059045 to U. Peters). C.M. Hutter was supported by a training grant from the National Cancer Institute, Institutes of Health, U.S. Department of Health and Human Services (R25 CA094880). Additional funding for this work was provided by National Cancer Institute, National Institutes of Health, U.S. Department of Health and Human Services (U01 CA137088 to U. Peters).; HPFS was supported by the NIH (P01 CA 055075 to C. S. Fuchs, R01 137178 to A. T. Chan, and P50 CA 127003 to C. S. Fuchs), NHS by the NIH (R01 137178 to A.T. Chan., P50 CA 127003 to C. S. Fuchs., and P01 CA 087969 to E. L. Giovannucci) and PHS by the NIH (CA41281).; PLCO was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, the Division of Cancer Prevention, National Cancer Institute, NIH, U.S. Department of Health and Human Services.; Control samples were genotyped as part of the Cancer Genetic Markers of Susceptibility (CGEMS) prostate cancer scan and were supported by the Intramural Research Program of the National Cancer Institute. The data sets used in this analysis were accessed with appropriate approval through the dbGaP online resource (54) through dbGaP accession number 000207v.1p1.c1 (20). Control samples were also genotyped as part of the GWAS of Lung Cancer and Smoking. This work was supported by NIH, Genes, Environment and Health Initiative [NIH GEI] (Z01 CP 010200). The human subjects participating in the GWAS are derived from the Prostate, Lung, Colon and Ovarian Screening Trial and the study is supported by intramural resources of the National Cancer Institute. Assistance with genotype cleaning, as well as with general study coordination, was provided by the Gene Environment Association Studies, GENEVA Coordinating Center (U01 HG004446). Assistance with data cleaning was provided by the National Center for Biotechnology Information. Funding support for genotyping, which was carried out at the Johns Hopkins University Center for Inherited Disease Research, was provided by the NIH GEI (U01 HG 004438). The data sets used for the analyses described in this article were obtained from dbGaP through National Center for Biotechnology Information (55), through dbGaP accession number ph000093.v2.p2.c1. The WHI program was supported by the National Heart, Lung, and Blood Institute, NIH, U. S. Department of Health and Human Services through contracts HHSN268201100001C-4C, HHSN268201100046C and HHSN271201100004C, and NO1WH4421. NR 55 TC 64 Z9 67 U1 0 U2 22 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 IS 8 BP 2036 EP 2044 DI 10.1158/0008-5472.CAN-11-4067 PG 9 WC Oncology SC Oncology GA 927KH UT WOS:000302905700015 PM 22367214 ER PT J AU Chernikova, SB Razorenova, OV Higgins, JP Sishc, BJ Nicolau, M Dorth, JA Chernikova, DA Kwok, S Brooks, JD Bailey, SM Game, JC Brown, JM AF Chernikova, Sophia B. Razorenova, Olga V. Higgins, John P. Sishc, Brock J. Nicolau, Monica Dorth, Jennifer A. Chernikova, Diana A. Kwok, Shirley Brooks, James D. Bailey, Susan M. Game, John C. Brown, J. Martin TI Deficiency in Mammalian Histone H2B Ubiquitin Ligase Bre1 (Rnf20/Rnf40) Leads to Replication Stress and Chromosomal Instability SO CANCER RESEARCH LA English DT Article ID DOUBLE-STRAND BREAKS; GERM-CELL TUMORS; GENE-EXPRESSION; HOMOLOGOUS RECOMBINATION; TESTICULAR CANCER; H3K4 METHYLATION; GENOME STABILITY; S-PHASE; REPAIR; UBIQUITYLATION AB Mammalian Bre1 complexes (BRE1A/B (RNF20/40) in humans and Bre1a/b (Rnf20/40) in mice) function similarly to their yeast homolog Bre1 as ubiquitin ligases in monoubiquitination of histone H2B. This ubiquitination facilitates methylation of histone H3 at K4 and K79, and accounts for the roles of Bre1 and its homologs in transcriptional regulation. Recent studies by others suggested that Bre1 acts as a tumor suppressor, augmenting expression of select tumor suppressor genes and suppressing select oncogenes. In this study, we present an additional mechanism of tumor suppression by Bre1 through maintenance of genomic stability. We track the evolution of genomic instability in Bre1-deficient cells from replication-associated double-strand breaks (DSB) to specific genomic rearrangements that explain a rapid increase in DNA content and trigger breakage-fusion-bridge cycles. We show that aberrant RNA-DNA structures (R-loops) constitute a significant source of DSBs in Bre1-deficient cells. Combined with a previously reported defect in homologous recombination, generation of R-loops is a likely initiator of replication stress and genomic instability in Bre1-deficient cells. We propose that genomic instability triggered by Bre1 deficiency may be an important early step that precedes acquisition of an invasive phenotype, as we find decreased levels of BRE1A/B and dimethylated H3K79 in testicular seminoma and in the premalignant lesion in situ carcinoma. Cancer Res; 72(8); 2111-9. (C) 2012 AACR. C1 [Chernikova, Sophia B.; Razorenova, Olga V.; Dorth, Jennifer A.; Game, John C.; Brown, J. Martin] Stanford Univ, Dept Radiat Oncol, Stanford, CA 94305 USA. [Higgins, John P.; Kwok, Shirley] Stanford Univ, Dept Pathol, Stanford, CA 94305 USA. [Nicolau, Monica] Stanford Univ, Dept Math, Stanford, CA 94305 USA. [Brooks, James D.] Stanford Univ, Dept Urol, Stanford, CA 94305 USA. [Sishc, Brock J.; Bailey, Susan M.] Colorado State Univ, Dept Environm & Radiol Hlth Sci, Ft Collins, CO 80523 USA. [Chernikova, Diana A.] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA. RP Brown, JM (reprint author), Stanford Univ, Dept Radiat Oncol, Stanford, CA 94305 USA. EM mbrown@stanford.edu FU National Cancer Institute [CA67166] FX The work was supported by grant CA67166 awarded to JMB by the National Cancer Institute. NR 50 TC 36 Z9 36 U1 0 U2 6 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 IS 8 BP 2111 EP 2119 DI 10.1158/0008-5472.CAN-11-2209 PG 9 WC Oncology SC Oncology GA 927KH UT WOS:000302905700022 PM 22354749 ER PT J AU Wilsker, D Chung, JH Bunz, F AF Wilsker, Deborah Chung, Jon H. Bunz, Fred TI Chk1 suppresses bypass of mitosis and tetraploidization in p53-deficient cancer cells SO CELL CYCLE LA English DT Article DE p53; Chk1; tetraploid; mitosis ID DNA-DAMAGE CHECKPOINT; PROTEIN PHOSPHATASE 2A; HUMAN SOMATIC-CELLS; CHROMOSOMAL INSTABILITY; MAMMALIAN-CELLS; GENOMIC INSTABILITY; GENETIC INSTABILITY; KINASE 1; ATR; PHOSPHORYLATION AB Many cancer cells are unable to maintain a numerically stable chromosome complement. It is well established that aberrant cell division can generate progeny with increased ploidy, but the genetic factors required for maintenance of diploidy are not well understood. Using an isogenic model system derived by gene targeting, we examined the role of Chk1 in p53-proficient and -deficient cancer cells. Targeted inactivation of a single CHK1 allele in stably diploid cells caused an elevated frequency of mitotic bypass if p53 was naturally mutated or experimentally disrupted by homologous recombination. CHK1-haploinsufficient, p53-deficient cells frequently underwent sequential rounds of DNA synthesis without an intervening mitosis. These aberrant cell cycles resulted in whole-genome endoreduplication and tetraploidization. The unscheduled bypass of mitosis could be suppressed by targeted reversion of a p53 mutation or by exogenous expression of Cdk1. In contrast, the number of tetraploid cells was not increased in isogenic cell populations that harbor hypomorphic ATR mutations, suggesting that suppression of unscheduled mitotic bypass is a distinct function of Chk1. These results are consistent with a recently described role for Chk1 in promoting the expression of genes that promote cell cycle transitions and demonstrate how Chk1 might prevent tetraploidization during the cancer cell cycle. C1 [Chung, Jon H.; Bunz, Fred] Johns Hopkins Sch Med, Dept Radiat Oncol & Mol Radiat Sci, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21218 USA. [Wilsker, Deborah] NCI, Mol Radiat Therapeut Branch, SAIC Frederick, Frederick, MD 21701 USA. RP Bunz, F (reprint author), Johns Hopkins Sch Med, Dept Radiat Oncol & Mol Radiat Sci, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21218 USA. EM fredbunz@jhmi.edu OI Chung, Jon/0000-0003-0526-3670 FU Flight Attendant Medical Research Institute; National Cancer Institute [R01CA157535, F32CA119724] FX The authors gratefully acknowledge the expert assistance of the staff of the Kimmel Cancer Center Cell Imaging Core facility and the Flow Cytometry Core facility. We thank David Morgan for Cdk1 expression plasmids. Support for this work was provided by the Flight Attendant Medical Research Institute and the National Cancer Institute grants R01CA157535 (F.B.) and F32CA119724 (D.W.). NR 45 TC 6 Z9 7 U1 0 U2 9 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1538-4101 J9 CELL CYCLE JI Cell Cycle PD APR 15 PY 2012 VL 11 IS 8 BP 1564 EP 1572 DI 10.4161/cc.19944 PG 9 WC Cell Biology SC Cell Biology GA 930TC UT WOS:000303162100018 PM 22433954 ER PT J AU Futatsugi, A Utreras, E Rudrabhatla, P Jaffe, H Pant, HC Kulkarni, AB AF Futatsugi, Akira Utreras, Elias Rudrabhatla, Parvathi Jaffe, Howard Pant, Harish C. Kulkarni, Ashok B. TI Cyclin-dependent kinase 5 regulates E2F transcription factor through phosphorylation of Rb protein in neurons SO CELL CYCLE LA English DT Article DE Cdk5; phosphorylation; cell cycle; retinoblastoma protein; E2F transcription factor; cell death; neurons ID CELL-CYCLE; RETINOBLASTOMA PROTEIN; ALZHEIMERS-DISEASE; NUCLEAR-LOCALIZATION; ACTIVATOR P35; AMYLOID-BETA; DNA-DAMAGE; CDK5; DEATH; NEURODEGENERATION AB Recent studies have shown the involvement of cyclin-dependent kinase 5 (Cdk5) in cell cycle regulation in postmitotic neurons. In this study, we demonstrate that Cdk5 and its co-activator p35 were detected in the nuclear fraction in neurons and Cdk5/p35 phosphorylated retinoblastoma (Rb) protein, a key protein controlling cell cycle re-entry. Cdk5/p35 phosphorylates Rb at the sites similar to those phosphorylated by Cdk4 and Cdk2. Furthermore, increased Cdk5 activity elevates activity of E2F transcription factor, which can trigger cell cycle re-entry, leading to neuronal cell death. A normal Cdk5 activity in neurons did not induce E2F activation, suggesting that Cdk5 does not induce cell cycle re-entry under normal conditions. Taken together, these results indicate that Cdk5 can regulate cell cycle by its ability to phosphorylate Rb. Most importantly, increased Cdk5 activity induces cell cycle re-entry, which is especially detrimental for survival of postmitotic neurons. C1 [Futatsugi, Akira; Utreras, Elias; Kulkarni, Ashok B.] Natl Inst Dent & Craniofacial Res, Funct Genom Sect, Lab Cell & Dev Biol, NIH, Bethesda, MD USA. [Rudrabhatla, Parvathi; Pant, Harish C.] Natl Inst Neurol Disorders & Stroke, Neurochem Lab, NIH, Bethesda, MD USA. [Jaffe, Howard] Natl Inst Neurol Disorders & Stroke, Prot & Peptide Facil, NIH, Bethesda, MD USA. RP Kulkarni, AB (reprint author), Natl Inst Dent & Craniofacial Res, Funct Genom Sect, Lab Cell & Dev Biol, NIH, Bethesda, MD USA. EM ak40m@nih.gov RI utreras, elias/I-1038-2013 OI utreras, elias/0000-0002-1004-0466 FU Intramural Divisions of the National Institute of Dental and Craniofacial Research; National Institute of Neurological Disorders and Stroke, National Institutes of Health FX We would like to thank Dr. Sushil Rane for critical reading of the manuscript, Ms. Shelagh Powers for expert editorial assistance and Dr. Yoko Futatsugi for help in manuscript preparation. These studies were supported by the Intramural Divisions of the National Institute of Dental and Craniofacial Research and the National Institute of Neurological Disorders and Stroke, National Institutes of Health. NR 50 TC 18 Z9 18 U1 1 U2 3 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1538-4101 J9 CELL CYCLE JI Cell Cycle PD APR 15 PY 2012 VL 11 IS 8 BP 1603 EP 1610 DI 10.4161/cc.20009 PG 8 WC Cell Biology SC Cell Biology GA 930TC UT WOS:000303162100022 PM 22456337 ER PT J AU Bock, CW Larkin, JD AF Bock, Charles W. Larkin, Joseph D. TI Heats of formation for the boronic acids R-B(OH)(2) and boroxines R3B3O3 (R = H, Li, HBe, H2B, H3C, H2N, HO, F, and Cl) calculated at the G2, G3, and G4 levels of theory SO COMPUTATIONAL AND THEORETICAL CHEMISTRY LA English DT Article DE Boroxines; Boronic acids; Heats of formation; G2; G3; G4 ID CORRELATED MOLECULAR CALCULATIONS; ACTIVE THERMOCHEMICAL TABLES; GAUSSIAN-BASIS SETS; WAVE-FUNCTIONS; ALTERNATIVE APPROACH; ELECTRONIC-STRUCTURE; ENERGIES; ATOMS; BOND; HYDROCARBONS AB Boronic acids (R-B(OH)(2)) and their boroxine (R3B3O3) dehydration products have emerged as important classes of compounds with a multitude of diverse applications. However, the available heats of formation for these compounds are not always as accurate as would be required for further use. In this study the heats of formation at 298.15 K of R-B(OH)(2) and R3B3O3 (R = H, Li, HBe, H2B, H3C, H2N, HO, F, and Cl) have been calculated at the G2, G3[G3B3], and 04 levels of theory and used to determine the enthalpy changes for the dehydration reactions: 3R-B(OH)(2) -> R3B3O3 + 3H(2)O; comparisons are made with other rigorous levels of theory, e.g. CBS-Q[CBS-QB3] and W1U, as well as with experimental values wherever possible. Enthalpy changes for the dehydration reactions have also been calculated using second-order Moller-Plesset perturbation theory (MP2) with the Dunning-Woon correlation-consistent aug-cc-pVTZ and aug-cc-pVTZ basis sets, and B3LYP density functional theory with the 6-311++G(2df,2pd) basis set. With the exception of H2N-B(OH)2, the dehydration reactions are consistently predicted to be endothermic. Our results provide a cautionary note for the use of the B3LYP functional in the calculation of structures and energies of boronic acids and boroxines. Where comparisons could be made, the G4 and W1U predictions for the heats of formation of these boron compounds differ significantly. (C) 2012 Elsevier B.V. All rights reserved. C1 [Bock, Charles W.] Philadelphia Univ, Dept Chem & Biochem, Sch Sci & Hlth, Philadelphia, PA 19144 USA. [Larkin, Joseph D.] NHLBI, NIH, Bethesda, MD 20851 USA. RP Bock, CW (reprint author), Philadelphia Univ, Dept Chem & Biochem, Sch Sci & Hlth, Sch House Lane & Henry Ave, Philadelphia, PA 19144 USA. EM bock.charles@gmail.com FU NIH, NHLBI FX This research was supported in part (J.D.L.) by the Intramural Research Program of the NIH, NHLBI. The PQS Cluster Facility at Philadelphia University. The authors would like to acknowledge helpful discussions with Dr. George D. Markham. NR 83 TC 4 Z9 4 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2210-271X J9 COMPUT THEOR CHEM JI Comput. Theor. Chem. PD APR 15 PY 2012 VL 986 BP 35 EP 42 DI 10.1016/j.comptc.2012.02.007 PG 8 WC Chemistry, Physical SC Chemistry GA 929PY UT WOS:000303079100006 PM 24653975 ER PT J AU Adjemian, J Olivier, KN Seitz, AE Holland, SM Prevots, DR AF Adjemian, Jennifer Olivier, Kenneth N. Seitz, Amy E. Holland, Steven M. Prevots, D. Rebecca TI Prevalence of Nontuberculous Mycobacterial Lung Disease in US Medicare Beneficiaries SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article DE nontuberculous mycobacteria; pulmonary; epidemiology; prevalence; United States ID UNITED-STATES; AVIUM-COMPLEX; CANCER SURVIVAL; HIV-INFECTION; EPIDEMIOLOGY; CARE; TUBERCULOSIS; DISPARITIES; AUSTRALIA; QUALITY AB Rationale: Pulmonary nontuberculous mycobacteria (PNTM) are an important cause of morbidity among older adults in the United States, but national prevalence estimates are lacking. Objectives: To describe the prevalence and trends of PNTM disease among adults aged 65 years or older throughout the United States. Methods: A nationally representative 5% sample of Medicare Part B beneficiaries was analyzed from 1997 to 2007. Demographic and medical claims data were compiled and prevalence estimates for PNTM and selected comorbidities were calculated and trends over time evaluated. Logistic regression was used to identify demographic and geographic factors associated with PNTM. Measurements and Main Results: From 1997 to 2007, the annual prevalence significantly increased from 20 to 47 cases/100,000 persons, or 8.2% per year. The period prevalence was 112 cases/100,000 persons, although prevalence was twofold higher among Asians/Pacific Islanders than among whites (228 vs. 116 cases/100,000 persons). Western states had the highest period prevalence at 149 cases/100,000 persons, with Hawaii having the highest prevalence at 396 cases/100,000 persons, followed by southeastern states, which had a period prevalence of 131 cases/100,000 persons. PNTM cases had more comorbid conditions than noncases and were 40% more likely to die than noncases. Women were 1.4 times more likely to be a PNTM case than men. Relative to whites, Asians/Pacific Islanders were twice as likely to be a case, whereas blacks were half as likely. Conclusions: The prevalence of PNTM is increasing across all regions of the United States and among both men and women. Significant racial/ethnic and geographic differences suggest important gene-environment interactions. C1 [Adjemian, Jennifer; Olivier, Kenneth N.; Seitz, Amy E.; Holland, Steven M.; Prevots, D. Rebecca] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. RP Adjemian, J (reprint author), Qrts 15 B-1,8 West Dr,MSC 2665, Bethesda, MD 20892 USA. EM jennifer.adjemian@nih.gov FU Division of Intramural Research, National Institute of Allergy and Infectious Diseases FX Supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases. NR 52 TC 108 Z9 110 U1 1 U2 5 PU AMER THORACIC SOC PI NEW YORK PA 61 BROADWAY, FL 4, NEW YORK, NY 10006 USA SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR 15 PY 2012 VL 185 IS 8 BP 881 EP 886 DI 10.1164/rccm.201111-2016OC PG 6 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA 925MW UT WOS:000302766300017 PM 22312016 ER PT J AU Ramsey, BW Banks-Schlegel, S Accurso, FJ Boucher, RC Cutting, GR Engelhardt, JF Guggino, WB Karp, CL Knowles, MR Kolls, JK LiPuma, JJ Lynch, S McCray, PB Rubenstein, RC Singh, PK Sorscher, E Welsh, M AF Ramsey, Bonnie W. Banks-Schlegel, Susan Accurso, Frank J. Boucher, Richard C. Cutting, Garry R. Engelhardt, John F. Guggino, William B. Karp, Christopher L. Knowles, Michael R. Kolls, Jay K. LiPuma, John J. Lynch, Susan McCray, Paul B., Jr. Rubenstein, Ronald C. Singh, Pradeep K. Sorscher, Eric Welsh, Michael TI Future Directions in Early Cystic Fibrosis Lung Disease Research An NHLBI Workshop Report SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article DE cystic fibrosis; airway disease; innate immunity; microbiology; genetics ID PSEUDOMONAS-AERUGINOSA INFECTION; YOUNG-CHILDREN; CLINICAL-TRIALS; INFANTS; INFLAMMATION; MODEL; END; BIOMARKERS; CLEARANCE; MUTATIONS AB Since the 1989 discovery that mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause cystic fibrosis (CF), there has been substantial progress toward understanding the molecular basis for CF lung disease, leading to the discovery and development of new therapeutic approaches. However, the earliest impact of the loss of CFTR function on airway physiology and structure and its relationship to initial infection and inflammation are poorly understood. Universal newborn screening for CF in the United States represents an unprecedented opportunity for investigating CF clinical manifestations very early in life. Recently developed animal models with pulmonary phenotypic manifestations also provide a window into the early consequences of this genetic disorder. For these reasons, the National Heart, Lung, and Blood Institute (NHLBI) convened a working group of extramural experts, entitled "Future Research Directions in Early CF Lung Disease" on September 21-22, 2010, to identify future research directions of great promise in CF. The priority areas identified included (1) exploring pathogenic mechanisms of early CF lung disease; (2) leveraging newborn screening to elucidate the natural history of early lung disease; (3) developing a spectrum of biomarkers of early lung disease that reflects CF pathophysiology, clinical outcome, and response to treatment; (4) exploring the role of genetics/genomics (e.g., modifier genes, gene-environmental interactions, and epigenetics) in early CF pathogenesis; (5) defining early microbiological events in CF lung disease; and (6) elucidating the initial airway inflammatory, remodeling, and repair mechanisms in CF lung disease. C1 [Ramsey, Bonnie W.] Seattle Childrens Res Inst, CFFT Therapeut Dev Network Coordinating Ctr, Seattle, WA 98121 USA. [Ramsey, Bonnie W.; Singh, Pradeep K.] Univ Washington, Sch Med, Seattle, WA USA. [Banks-Schlegel, Susan] NHLBI, NIH, Bethesda, MD 20892 USA. [Accurso, Frank J.] Univ Colorado, Sch Med, Denver, CO USA. [Boucher, Richard C.; Knowles, Michael R.] Univ N Carolina, Sch Med, Chapel Hill, NC USA. [Cutting, Garry R.; Guggino, William B.] Johns Hopkins Sch Med, Baltimore, MD USA. [Engelhardt, John F.] Univ Iowa, Sch Med, Iowa City, IA 52242 USA. [Karp, Christopher L.] Univ Cincinnati, Coll Med, Cincinnati, OH USA. [Karp, Christopher L.] Cincinnati Childrens Hosp Res Fdn, Div Mol Immunol, Cincinnati, OH USA. [Kolls, Jay K.] Louisiana State Univ, Sch Med New Orleans, New Orleans, LA USA. [LiPuma, John J.] Univ Michigan, Sch Publ Hlth, Ann Arbor, MI 48109 USA. [Lynch, Susan] Univ Calif San Francisco, Sch Med, San Francisco, CA USA. [McCray, Paul B., Jr.] Univ Iowa, Childrens Hosp, Iowa City, IA USA. [Rubenstein, Ronald C.] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA. [Rubenstein, Ronald C.] Univ Penn, Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Sorscher, Eric] Univ Alabama Birmingham, Sch Med, Birmingham, AL USA. [Welsh, Michael] Univ Iowa, Howard Hughes Med Inst, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USA. RP Ramsey, BW (reprint author), Seattle Childrens Res Inst, CFFT Therapeut Dev Network Coordinating Ctr, 2001 8th Ave,Mailstop CW8-5B, Seattle, WA 98121 USA. EM bonnie.ramsey@seattlechildrens.org OI Rubenstein, Ronald/0000-0002-3138-4006 FU Division of Lung Diseases, National Institutes of Health, of the U.S. Department of Health and Human Services FX Supported by the Division of Lung Diseases, National Institutes of Health, of the U.S. Department of Health and Human Services. NR 51 TC 29 Z9 29 U1 0 U2 9 PU AMER THORACIC SOC PI NEW YORK PA 61 BROADWAY, FL 4, NEW YORK, NY 10006 USA SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR 15 PY 2012 VL 185 IS 8 BP 887 EP 892 DI 10.1164/rccm.201111-2068WS PG 6 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA 925MW UT WOS:000302766300018 PM 22312017 ER PT J AU Shaw, P AF Shaw, Philip TI Mapping Anomalies in Cortico-Striatal Trajectories in ADHD Using Structural MRI SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Shaw, Philip] NHGRI, Social & Behav Res Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 23 BP 7S EP 8S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000024 ER PT J AU Baumann, MH AF Baumann, Michael H. TI Neuropharmacology of Mephedrone and Methylone in the Rat SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE methcathinone; designer drugs; monoamines; transporters microdialysis C1 [Baumann, Michael H.] NIDA, IRP, NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 34 BP 11S EP 11S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000035 ER PT J AU Knowles, EEM Wesier, M David, AS Dickinson, D Gold, J Glahn, D Davidson, M Reichenberg, A AF Knowles, Emma E. M. Wesier, Mark David, Anthony S. Dickinson, Dwight Gold, James Glahn, David Davidson, Michael Reichenberg, Abraham TI The Puzzle of Processing Speed, Memory and Executive Functioning Impairments in Schizophrenia: Fitting the Pieces Together SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; Cognition; Executive Functioning; Processing Speed; Modelling C1 [Knowles, Emma E. M.; Glahn, David] Yale Univ, Dept Med, New Haven, CT 06520 USA. [Knowles, Emma E. M.; David, Anthony S.; Reichenberg, Abraham] Inst Psychiat, Dept Psychosis Studies, London, England. [Wesier, Mark; Davidson, Michael] Sheba Med Ctr, Div Psychiat, Ramat Gan, Israel. [Dickinson, Dwight] NIMH, Intramural Res Program, NIH, Clin Brain Disorders Branch,Genes Cognit & Psycho, Bethesda, MD 20892 USA. [Gold, James] Maryland Psychiat Res Ctr, Dept Psychiat, Baltimore, MD 21228 USA. RI David, Anthony/C-1315-2011 OI David, Anthony/0000-0003-0967-774X NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 92 BP 27S EP 27S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000087 ER PT J AU Pickens, CL Cifani, C Navarre, BM Eichenbaum, H Theberge, FR Baumann, MH Calu, DJ Shaham, Y AF Pickens, Charles L. Cifani, Carlo Navarre, Brittany M. Eichenbaum, Hila Theberge, Florence R. Baumann, Michael H. Calu, Donna J. Shaham, Yavin TI Effect of Fenfluramine on Reinstatement of Food Seeking in Female and Male Rats: Implications for the Predictive Validity of the Reinstatement Model SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Relapse; Fenfluramine; Diet; Predictive validity; Reinstatement C1 [Pickens, Charles L.; Cifani, Carlo; Navarre, Brittany M.; Eichenbaum, Hila; Theberge, Florence R.; Calu, Donna J.; Shaham, Yavin] NIDA, Behav Neurosci Branch, Baltimore, MD USA. [Baumann, Michael H.] NIDA, Chem Biol Branch, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 126 BP 37S EP 37S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000121 ER PT J AU Graybeal, CM Plitt, A Choi, DC Saksida, LM Bussey, TJ Theberge, FRM Lui, QR Shaham, Y Ressler, KJ Holmes, A AF Graybeal, Carolyn M. Plitt, Aaron Choi, Dennis C. Saksida, Lisa M. Bussey, Timothy J. Theberge, Florence R. M. Lui, Qing-Rong Shaham, Yavin Ressler, Kerry J. Holmes, Andrew TI The Role of Prefrontal Brain-Derived Neurotrophic Factor in Modulating Stress-induced Facilitation of Reversal Learning SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Reversal learning; Stress; Brain-derived neurotrophic factor; Executive function C1 [Graybeal, Carolyn M.; Plitt, Aaron; Holmes, Andrew] NIAAA, NIH, Rockville, DC USA. [Choi, Dennis C.; Ressler, Kerry J.] Emory Univ, Sch Med, Dept Psychiat & Behav Sci, Atlanta, GA USA. [Saksida, Lisa M.; Bussey, Timothy J.] Univ Cambridge, Dept Expt Psychol, Cambridge CB2 3EB, England. [Theberge, Florence R. M.; Lui, Qing-Rong; Shaham, Yavin] NIDA, NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 129 BP 38S EP 38S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000124 ER PT J AU Daut, RA DeBrouse, LM Noronha, B Holmes, A AF Daut, Rachel A. DeBrouse, Lauren M. Noronha, Bianca Holmes, Andrew TI Effects of Chronic Intermittent Ethanol Exposure on Discrimination and Reversal Learning in Mice SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE ethanol; cognitive flexibility; touchscreen; discrimination; reversal C1 [Daut, Rachel A.; DeBrouse, Lauren M.; Noronha, Bianca; Holmes, Andrew] NIAAA, Lab Behav & Genom Neurosci, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 141 BP 42S EP 42S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000136 ER PT J AU Myal, S Wang, B Wise, RA AF Myal, Stephanie Wang, Bin Wise, Roy A. TI Extinction with Cocaine Methiodide Attenuates Priming-Induced, but not Footshock-Induced, Reinstatement of Cocaine Seeking SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE cocaine; self-administration; stress; addiction; drug abuse C1 [Myal, Stephanie; Wang, Bin; Wise, Roy A.] NIDA, Behav Neurosci Res Branch, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 142 BP 42S EP 43S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000137 ER PT J AU Tripp, AC Oh, H Guilloux, JP Martinowich, K Lewis, D Sibille, EL AF Tripp, Adam C. Oh, Hyunjung Guilloux, Jean-Philippe Martinowich, Keri Lewis, David Sibille, Etienne L. TI BDNF- and GABA- Related Gene Dysregulation in the Anterior Cingulate Cortex in Major Depressive Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE BDNF; GABA; Anterior Cingulate Cortex; postmortem subjects; mouse model C1 [Oh, Hyunjung; Lewis, David; Sibille, Etienne L.] Univ Pittsburgh, Med Ctr, Ctr Neurosci, Pittsburgh, PA USA. [Guilloux, Jean-Philippe] Univ Paris 11, Paris, France. [Martinowich, Keri] NIMH, Genes Cognit & Psychosis Program, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 169 BP 51S EP 51S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000164 ER PT J AU Masdeu, JC Gonzalez-Pinto, A Matute, C Ruiz-de-Azua, S Palomino, A De Leon, J Dalmau, J AF Masdeu, Joseph C. Gonzalez-Pinto, Ana Matute, Carlos Ruiz-de-Azua, Sonia Palomino, Aitor De Leon, Jose Dalmau, Josep TI Serum IgG Antibodies Against the NMDA Receptor Not Detected in Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; NMDA receptor; Antibodies C1 [Masdeu, Joseph C.] NIMH, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. [Gonzalez-Pinto, Ana; Ruiz-de-Azua, Sonia] Univ Basque Country, Biomed Res Ctr Mental Hlth Net CIBERSAM, Santiago Apostol Hosp, Vitoria, Spain. [Matute, Carlos; Palomino, Aitor] Univ Basque Country, CIBERNED, Dept Neurociencias, Fac Med, Leioa, Spain. [De Leon, Jose] Univ Kentucky, Dept Psychiat, Lexington, KY USA. [Dalmau, Josep] Univ Barcelona, ICREA, IDIBAPS, Neurol Serv,Hosp Clin, Barcelona, Spain. RI de Leon, Jose/F-2709-2013 OI de Leon, Jose/0000-0002-7756-2314 NR 0 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 175 BP 53S EP 53S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000170 ER PT J AU Paterson, C Wang, YH Law, AJ AF Paterson, Clare Wang, Yanhong Law, Amanda J. TI Cellular, Regional and Biochemical Characterization of Neuregulin 3 in the CNS: Implications for a Functional Role in Schizophrenia Pathophysiology SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; Neuregulin; Neurodevelopment; PIK3 C1 [Paterson, Clare; Wang, Yanhong; Law, Amanda J.] NIMH, NIH, Bethesda, MD 20892 USA. RI Law, Amanda/G-6372-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 176 BP 53S EP 53S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000171 ER PT J AU Machado-Vieira, R Luckenbaugh, DA Soeiro-De-Souza, MG Busnello, JV Gattaz, WF Zarate, CA AF Machado-Vieira, Rodrigo Luckenbaugh, David A. Soeiro-De-Souza, Marcia G. Busnello, Joao V. Gattaz, Wagner F. Zarate, Carlos A., Jr. TI Early Improvement with Lithium in Classic Mania Predicts Later Response SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE mania; bipolar disorder; lithium; clinical trial; predictors C1 [Machado-Vieira, Rodrigo; Gattaz, Wagner F.] Univ Sao Paulo, Inst Psychiat, Sao Paulo, Brazil. [Machado-Vieira, Rodrigo; Soeiro-De-Souza, Marcia G.; Gattaz, Wagner F.] Univ Sao Paulo, Dept Psychiat, Sao Paulo, Brazil. [Luckenbaugh, David A.; Zarate, Carlos A., Jr.] NIMH, Expt Therapeut & Pathophysiol Branch, NIH, Bethesda, MD 20892 USA. [Busnello, Joao V.] Univ Chicago, Dept Psychiat, Sao Paulo, IL USA. RI MACHADO-VIEIRA, RODRIGO/D-8293-2012; Gattaz, Wagner/C-4456-2012 OI MACHADO-VIEIRA, RODRIGO/0000-0002-4830-1190; NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 191 BP 58S EP 58S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000186 ER PT J AU Yates, W Feighery, EL Mattay, VS Apud, JA Berman, KF Weinberger, DR Callicott, JH AF Yates, William Feighery, Emer L. Mattay, Venkata S. Apud, Jose A. Berman, Karen F. Weinberger, Daniel R. Callicott, Joseph H. TI Is the Prefrontal Inefficiency Phenotype in Schizophrenic Patients Present in an Un-Medicated State? SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; Working Memory; Inefficiency; fMRI C1 [Yates, William; Feighery, Emer L.; Mattay, Venkata S.; Apud, Jose A.; Berman, Karen F.; Callicott, Joseph H.] NIMH, Clin Brain Disorders Branch, DIRP, NIH, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 206 BP 63S EP 63S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000201 ER PT J AU Fortgang, R Owrutsky, Z Weinberger, DR Dickinson, D AF Fortgang, Rebecca Owrutsky, Zoe Weinberger, Daniel R. Dickinson, Dwight TI Suicidality and Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; Suicide; Self-Injury C1 [Fortgang, Rebecca; Weinberger, Daniel R.; Dickinson, Dwight] NIMH, CBDB, Bethesda, MD 20892 USA. [Owrutsky, Zoe] Univ Pittsburgh, Dept Psychol, Pittsburgh, PA 15260 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 209 BP 64S EP 64S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000204 ER PT J AU Schaefer, JD Weinberger, DR Dickinson, D AF Schaefer, Jonathan D. Weinberger, Daniel R. Dickinson, Dwight TI Generalized Cognitive Impairment in Schizophrenia: An Update and Extension of Previous Meta-Analyses SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE schizophrenia; meta-analysis; cognitive; impairment; generalized C1 [Schaefer, Jonathan D.; Weinberger, Daniel R.; Dickinson, Dwight] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 210 BP 64S EP 65S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000205 ER PT J AU Costa, VD Averbeck, BB AF Costa, Vincent D. Averbeck, Bruno B. TI Emotional Perception Modulates Activity in the Human Periaqueductal Gray SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE emotion; fMRI; periaqueductal gray; amygdala; perception C1 [Costa, Vincent D.; Averbeck, Bruno B.] NIMH, Neuropsychol Lab, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 219 BP 67S EP 67S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000214 ER PT J AU Romer, AL Jarcho, JM Shechner, T Pine, DS Nelson, EE AF Romer, Adrienne L. Jarcho, Johanna M. Shechner, Tomer Pine, Daniel S. Nelson, Eric E. TI Socially-Driven Cognitive Biases in Expectations and Recall of Peer Rejection in Anxiety SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE cognitive biases; anxiety; self-esteem; peer rejection; chatroom C1 [Romer, Adrienne L.; Jarcho, Johanna M.; Shechner, Tomer; Pine, Daniel S.; Nelson, Eric E.] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 218 BP 67S EP 67S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000213 ER PT J AU Adalio, CJ White, SF Fowler, KA Sinclair, S Pine, DS Blair, RJR AF Adalio, Christopher J. White, Stuart F. Fowler, Katherine A. Sinclair, Stephen Pine, Daniel S. Blair, R. J. R. TI Youth with Disruptive Behavior Disorders and Callous-Unemotional Traits Show Impaired Neural Representation of Expected Value SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Disruptive Behavior Disorder; Conduct Disorder; Decision-making; fMRl C1 [Adalio, Christopher J.; White, Stuart F.; Sinclair, Stephen; Blair, R. J. R.] NIMH, Unit Affect Cognit Neurosci, Bethesda, MD 20892 USA. [Fowler, Katherine A.] Epidem Intelligence Serv, CDC, Atlanta, GA USA. [Pine, Daniel S.] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 232 BP 71S EP 72S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000227 ER PT J AU White, S Fowler, K Sinclair, S Pine, DS Blair, JR AF White, Stuart Fowler, Katherine Sinclair, Stephen Pine, Daniel S. Blair, James R. TI Youth with Disruptive Behavior Disorders and Callous-Unemotional Traits Show Impaired Neural Representation of Expected Value SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Conduct Disorder; Callous-Unemotional Traits; neuroscience; learning C1 [White, Stuart; Fowler, Katherine; Sinclair, Stephen; Blair, James R.] NIMH, Unit Affect Cognit Neurosci, Bethesda, MD 20892 USA. [Pine, Daniel S.] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 231 BP 71S EP 71S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000226 ER PT J AU Franco-Chaves, JA Mateus, CF Letkiewicz, AM Leberman, LN Zarate, CA Grillon, C AF Franco-Chaves, Jose A. Mateus, Camilo F. Letkiewicz, Allison M. Leberman, Lynne N. Zarate, Carlos A. Grillon, Christian TI Increased Startle to Contextual Threat in Unipolar Depression SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Startle; Depression; Contextual Threat C1 [Franco-Chaves, Jose A.; Mateus, Camilo F.; Zarate, Carlos A.] NIMH, ETPB, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 252 BP 78S EP 78S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000247 ER PT J AU Hochheiser, J Deep-Soboslay, A Dickinson, D Bliss, L Apud, J Weinberger, D Hyde, T AF Hochheiser, Jesse Deep-Soboslay, Amy Dickinson, Dwight Bliss, Lindsay Apud, Jose Weinberger, Daniel Hyde, Thomas TI Complex Motor Sequencing Deficits are Associated with Specific Cognitive Abnormalities among Patients with Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE complex motor sequencing; schizophrenia; neurological evaluation scale; neuropsychological; frontostriatal C1 [Hochheiser, Jesse; Deep-Soboslay, Amy; Dickinson, Dwight; Bliss, Lindsay; Apud, Jose; Weinberger, Daniel; Hyde, Thomas] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Weinberger, Daniel; Hyde, Thomas] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 284 BP 88S EP 88S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000279 ER PT J AU David, CN Maher, K Tan, M Tossell, J Weisinger, B Gochman, P Miller, R Greenstein, D Overman, G Rapoport, JL Gogtay, N AF David, Christopher N. Maher, Kirstin Tan, Marcus Tossell, Julia Weisinger, Brian Gochman, Peter Miller, Rachel Greenstein, Deanna Overman, Gerald Rapoport, Judith L. Gogtay, Nitin TI Risk Factors for Neutropenia in Clozapine-Treated Children and Adolescents with Childhood-Onset Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE clozapine; neutropenia; childhood-onset schizophrenia C1 [David, Christopher N.; Maher, Kirstin; Tan, Marcus; Tossell, Julia; Weisinger, Brian; Gochman, Peter; Miller, Rachel; Greenstein, Deanna; Overman, Gerald; Rapoport, Judith L.; Gogtay, Nitin] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 329 BP 102S EP 102S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000324 ER PT J AU Ungerleider, LG AF Ungerleider, Leslie G. TI Circuits Mediating Different Psychological Domains - Face Perception SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat ID TEMPORAL CORTEX; SELECTIVITY C1 [Ungerleider, Leslie G.] NIMH, Lab Brain & Cognit, NIH, Bethesda, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 346 BP 108S EP 108S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000341 ER PT J AU Hibbeln, J AF Hibbeln, Joseph TI Suicide Deaths of Active-Duty US Military and Omega-3. Fatty-Acid Status: A Case-Control Comparison SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Hibbeln, Joseph] NIAAA, Rockville, MD 20852 USA. NR 0 TC 1 Z9 1 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 366 BP 114S EP 114S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000361 ER PT J AU Rapoport, SI AF Rapoport, Stanley I. TI Evidence for a Role of Arachidonic Acid in Bipolar Disorders and its Treatment SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Rapoport, Stanley I.] NIA, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 365 BP 114S EP 114S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000360 ER PT J AU Gogtay, N AF Gogtay, Nitin TI Exploring Neurodevelopmental Networks in Childhood Onset Schizophrenia: An Overview from Structural Neuroimaging SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE childhood; schizophrenia; neuroimaging C1 [Gogtay, Nitin] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 375 BP 116S EP 117S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000370 ER PT J AU James, B AF James, Blair TI The Neuro-Computational Underpinnings of the Decision-Making Deficit in Conduct Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Conduct Disorder; Amygdala; Prediction error; Expected value; Ventromedial prefrontal cortex C1 [James, Blair] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 409 BP 127S EP 127S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000404 ER PT J AU Cornwell, BR AF Cornwell, Brian R. TI Evidence that Cortical Plasticity Underlies Ketamine's Rapid Antidepressant Action SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE cortical excitability; gamma oscillation; ketamine; major depression; magnetoencephalography C1 [Cornwell, Brian R.] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 419 BP 130S EP 130S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000414 ER PT J AU Blair, KS Teng, CY Geraci, M Blair, J Pine, DS AF Blair, Karina S. Teng, Cindy Y. Geraci, Marilla Blair, James Pine, Daniel S. TI Reduced Dorsal Anterior Cingulate Cortical Activity during Explicit Emotional Regulation and as a Function of Top Down Attentional Control in Generalized Social Phobia (GSP), Generalized Anxiety Disorder (GAD) and Comorbid GSP/GAD SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE social phobia; generalized anxiety disorder; emotional regulation; fMRI; attention C1 [Blair, Karina S.; Teng, Cindy Y.; Geraci, Marilla; Blair, James; Pine, Daniel S.] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 3 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 433 BP 134S EP 135S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000428 ER PT J AU Geerlings, MI Sigurdsson, S Eiriksdottir, G Garcia, ME Harris, TB Gudnason, V Launer, LJ AF Geerlings, Mirjam I. Sigurdsson, Sigurdur Eiriksdottir, Gudny Garcia, Melissa E. Harris, Tamara B. Gudnason, Vilmundur Launer, Lenore J. TI Major Depressive Disorder, Salivary Cortisol, and Brain Atrophy in a Population-Based Cohort of Old Persons without Dementia. The AGES-Reykjavik Study SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE brain; cortisol; depression; MRI; population-based C1 [Geerlings, Mirjam I.; Garcia, Melissa E.; Harris, Tamara B.; Launer, Lenore J.] NIA, Lab Epidemiol Demog & Biometry, Bethesda, MD 20892 USA. [Geerlings, Mirjam I.] Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands. [Sigurdsson, Sigurdur; Eiriksdottir, Gudny; Gudnason, Vilmundur] Iceland Heart Assoc, Kopavogur, Iceland. [Gudnason, Vilmundur] Univ Iceland, Reykjavik, Iceland. RI Gudnason, Vilmundur/K-6885-2015 OI Gudnason, Vilmundur/0000-0001-5696-0084 NR 0 TC 0 Z9 0 U1 0 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 446 BP 139S EP 139S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000441 ER PT J AU Murphy, E Hawariat, G McMahon, F AF Murphy, Eleanor Hawariat, Girma McMahon, Francis TI Genetic Ancestry, Self-Reported Race and Therapeutic Response to Antidepressant Medication: Additional highlights from the STAR(star)D Study SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Psychopharmacology; Ethnicity; Genetics; Depression; Disparity C1 [Murphy, Eleanor; Hawariat, Girma; McMahon, Francis] NIH, Human Genet Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 447 BP 139S EP 140S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000442 ER PT J AU Flynn, S Cinar, OG Sibille, E Holmes, A AF Flynn, Shaun Cinar, Ozge Gunduz Sibille, Etienne Holmes, Andrew TI Amygdala Gene Networks Associated with Mouse Trait Differences in Fear Extinction SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE impaired extinction; behavior; fear; gene expressions; stress C1 [Flynn, Shaun; Cinar, Ozge Gunduz; Holmes, Andrew] NIAAA, Lab Behav & Genom Neurosci, Bethesda, MD 15260 USA. [Sibille, Etienne] Univ Pittsburgh, Ctr Neurosci, Dept Psychiat, Pittsburgh, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 465 BP 145S EP 145S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000460 ER PT J AU Palumbo, S Law, A AF Palumbo, Sara Law, Amanda TI AKT-2 Heterozygous Mice Exhibit a Unique Behavioral Phenotype Relevant to Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE AKT-2; schizophrenia; behavior; mouse; heterozygous C1 [Palumbo, Sara; Law, Amanda] NIH, Clin Brain Disorder Branch, Bethesda, MD 20892 USA. RI Law, Amanda/G-6372-2012; palumbo, sara/B-1603-2013 OI palumbo, sara/0000-0002-3809-6058 NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 466 BP 145S EP 145S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000461 ER PT J AU Kolata, S Rompala, G Jiang, ZH Nakao, K Paredes, DA Nakazawa, K AF Kolata, Stefan Rompala, Gregory Jiang, Zhihong Nakao, Kazuhito Paredes, Daniel A. Nakazawa, Kazu TI Postnatal Gad67 Ablation in a Subset of Corticolimbic Interneurons Confers an Anhedonia-Like Phenotype SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Gad67; Interneurons; Anhedonia; Dopamine; Social Withdrawal C1 [Kolata, Stefan; Rompala, Gregory; Jiang, Zhihong; Nakao, Kazuhito; Nakazawa, Kazu] NIMH, NIH, Bethesda, MD 20892 USA. [Paredes, Daniel A.] NINDS, NIH, Bethesda, MD 20892 USA. RI Paredes , Daniel/L-6610-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 467 BP 146S EP 146S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000462 ER PT J AU Nakao, K Nakazawa, K AF Nakao, Kazuhito Nakazawa, Kazu TI Postnatal NMDA Receptor Deletion in Corticolimbic GABAergic Interneurons Attenuates Gamma Oscillation SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE NMDA; gamma oscillatory; internueron; auditory cortex; somatosensory cortex C1 [Nakao, Kazuhito; Nakazawa, Kazu] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 468 BP 146S EP 146S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000463 ER PT J AU New, AS Perez-Rodriguez, MM Ripoll, L Bevilacqua, L Yuan, QP Zhou, ZF Hodgkinson, C Goodman, M Koenigsberg, HW Goldman, D Siever, LJ AF New, Antonia S. Perez-Rodriguez, M. Mercedes Ripoll, Luis Bevilacqua, Laura Yuan, Qiaoping Zhou, Zhifeng Hodgkinson, Colin Goodman, Marianne Koenigsberg, Harold W. Goldman, David Siever, Larry J. TI Genetic Variation in OPRM1 Gene and Anxious Attachment in Personality Disorders SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Opioid; attachment; borderline personality; interpersonal; genetic C1 [New, Antonia S.; Perez-Rodriguez, M. Mercedes; Ripoll, Luis; Goodman, Marianne; Koenigsberg, Harold W.; Siever, Larry J.] Mt Sinai Sch Med, New York, NY USA. [Bevilacqua, Laura; Yuan, Qiaoping; Zhou, Zhifeng; Hodgkinson, Colin; Goldman, David] NIAAA, Neurogenet Lab, Washington, DC USA. RI Perez Rodriguez, Maria/B-9410-2013 OI Perez Rodriguez, Maria/0000-0001-5137-1993 NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 491 BP 154S EP 155S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000486 ER PT J AU Perez-Rodriguez, MM Roussos, P Bevilacqua, L Yuan, QP Zhou, ZF Hodgkinson, C Ripoll, L Goodman, M Koenigsberg, HW Goldman, D Siever, LJ New, AS AF Perez-Rodriguez, M. Mercedes Roussos, Panos Bevilacqua, Laura Yuan, Qiaoping Zhou, Zhifeng Hodgkinson, Colin Ripoll, Luis Goodman, Marianne Koenigsberg, Harold W. Goldman, David Siever, Larry J. New, Antonia S. TI Exploratory Association Study of 130 Candidate Genes in Patients with Borderline Personality Disorder and Healthy Controls SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Borderline Personality Disorder; Genetics; Dopamine beta hydroxylase; Association study C1 [Perez-Rodriguez, M. Mercedes; Roussos, Panos; Ripoll, Luis; Goodman, Marianne; Koenigsberg, Harold W.; Siever, Larry J.; New, Antonia S.] Mt Sinai Sch Med, New York, NY USA. [Perez-Rodriguez, M. Mercedes; Roussos, Panos; Ripoll, Luis; Goodman, Marianne; Koenigsberg, Harold W.; Siever, Larry J.; New, Antonia S.] James J Peters Vet Affairs Med Ctr, Mental Hlth Patient Care Ctr, Bronx, NY USA. [Bevilacqua, Laura; Yuan, Qiaoping; Zhou, Zhifeng; Hodgkinson, Colin; Goldman, David] NIAAA, Neurogenet Lab, NIH, Rockville, MD 20852 USA. RI Perez Rodriguez, Maria/B-9410-2013; Roussos, Panos/J-7090-2013 OI Perez Rodriguez, Maria/0000-0001-5137-1993; Roussos, Panos/0000-0002-4640-6239 NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 492 BP 155S EP 155S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000487 ER PT J AU Chen, DT Akula, N Hou, LP Hawariat, G Detera-Wadleigh, S Jiang, X McMahon, FJ AF Chen, David T. Akula, Nirmala Hou, Liping Hawariat, Girma Detera-Wadleigh, Sevilla Jiang, Xueying McMahon, Francis J. CA BIGS Consortium TI Genome-Wide Association Signals in Bipolar Disorder are Enriched for Genetic Variants within Transcription Factor Binding Sites and Expression Quantitative Trait Loci SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE bipolar disorder; transcription factor binding site; cis-regulation; enrichment C1 [Chen, David T.; Akula, Nirmala; Hou, Liping; Hawariat, Girma; Detera-Wadleigh, Sevilla; Jiang, Xueying; McMahon, Francis J.] NIMH, Human Genet Branch, NIH, Bethesda, MD 20892 USA. [BIGS Consortium] Univ Calif San Diego, Dept Psychiat, San Diego, CA 92103 USA. RI Hou, Liping/G-1648-2011 OI Hou, Liping/0000-0003-3972-245X NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 496 BP 156S EP 156S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000491 ER PT J AU Zheutlin, AB Callicotti, JH Yates, WD Chen, Q Weinberger, DR Wang, KH AF Zheutlin, Amanda B. Callicotti, Joseph H. Yates, William D. Chen, Qiang Weinberger, Daniel R. Wang, Kuan H. TI ARC Genotype Predicts Prefrontal Efficiency and Connectivity Between Prefrontal Cortex and Hippocampus SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE schizophrenia; cunnectivity; prefrontal cortex; genetics; ARC C1 [Zheutlin, Amanda B.; Callicotti, Joseph H.; Yates, William D.; Chen, Qiang; Wang, Kuan H.] NIMH, Clin Brain Disorders Branch, DIRP, NIH, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 501 BP 158S EP 158S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000496 ER PT J AU Baranger, DAA Yates, WD Chen, Q Mattay, VS Weinberger, DR Callicott, JH AF Baranger, David A. A. Yates, William D. Chen, Qiang Mattay, Venkata S. Weinberger, Daniel R. Callicott, Joseph H. TI Disci Leu607Phe and Ser704Cys Reduce Prefrontal-Hippocampal Coupling During a Working Memory Task SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE DISCI; Connectivity; Schizophrenia; Working Memory; Hippocampus C1 [Baranger, David A. A.; Yates, William D.; Chen, Qiang; Mattay, Venkata S.; Weinberger, Daniel R.; Callicott, Joseph H.] NIMH, Genes Cognit & Psychosis Program, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 507 BP 160S EP 160S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000502 ER PT J AU Wallwork, RS Straub, RE Weinberger, DR Dickinson, D AF Wallwork, Rachel S. Straub, Richard E. Weinberger, Daniel R. Dickinson, Dwight TI Investigation of The Relationship of KYNU and Cortical Kynurenine Pathway Metabolism to Cognition in Schizophrenia and Healthy Volunteers SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Kynurenine; cognition; schizophrenia C1 [Wallwork, Rachel S.; Straub, Richard E.; Dickinson, Dwight] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] Johns Hopkins Med Ctr, Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 513 BP 162S EP 162S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000508 ER PT J AU Enoch, MA Zhou, ZF Mash, DC Yuan, QP Goldman, D Sinha, R AF Enoch, Mary-Anne Zhou, Zhifeng Mash, Deborah C. Yuan, Qiaoping Goldman, David Sinha, Rajita TI Genetic Influences on HPA Axis Reactivity and Hippocampal Expression of Stress-Related Genes in Alcoholics and Cocaine Addicts SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE cortisol; CRH; CRHBP; FKBP5; NPY C1 [Enoch, Mary-Anne; Zhou, Zhifeng; Yuan, Qiaoping; Goldman, David] NIAAA, LNG, NIH, Bethesda, MD USA. [Mash, Deborah C.] Univ Miami, Sch Med, Dept Neurol, Miami, FL USA. [Sinha, Rajita] Yale Univ, Sch Med, Yale Stress Ctr, New Haven, CT USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 516 BP 163S EP 163S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000511 ER PT J AU Damme, KSF Wei, SM Baller, EB Bloch, JB Kohn, PD Kippenhan, JS Rubinow, DR Martinez, P Schmidt, PJ Berman, KF AF Damme, Katherine S. F. Wei, Shau-Ming Baller, Erica B. Bloch, Jeff B. Kohn, Philip D. Kippenhan, Jonathon S. Rubinow, David R. Martinez, Pedro Schmidt, Peter J. Berman, Karen F. TI Ovarian Hormones Modulate Age-Related Changes in rCBF during Working Memory - A Positron Emission Tomography (PET) Study in Women SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Imaging; Healthy Aging; Estrogen; Hippocampus; PET C1 [Damme, Katherine S. F.; Wei, Shau-Ming; Baller, Erica B.; Bloch, Jeff B.; Kohn, Philip D.; Kippenhan, Jonathon S.; Martinez, Pedro; Schmidt, Peter J.; Berman, Karen F.] NIMH, Bethesda, MD 20892 USA. [Rubinow, David R.] Univ N Carolina, Sch Med, Chapel Hill, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 540 BP 170S EP 170S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000535 ER PT J AU Killgore, WDS Britton, JC Rosso, IM Schwab, ZJ Rauch, SL AF Killgore, William D. S. Britton, Jennifer C. Rosso, Isabelle M. Schwab, Zachary J. Rauch, Scott L. TI Shared and Unique Patterns of Cortico-Limbic Activation Across Anxiety Disorders SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Neuroimaging; Panic Disorder; PTSD; Phobia; Amygdala C1 [Killgore, William D. S.; Rosso, Isabelle M.; Schwab, Zachary J.; Rauch, Scott L.] McLean Hosp, Ctr Depress Anxiety & Stress Res, Belmont, MA 02178 USA. [Britton, Jennifer C.] NIMH, NIH, Bethesda, MD 20892 USA. RI Britton, Jennifer/J-4501-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 549 BP 173S EP 173S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000544 ER PT J AU Roy, A Carlisi, C Daniele, T Pine, D Ernst, M AF Roy, Amy Carlisi, Christina Daniele, Teresa Pine, Daniel Ernst, Monique TI Val/Met Polymorphism of the BDNF Gene and Altered Resting-State Functional Connectivity in Healthy Adults SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Resting-State; BDNF; fMRI; Anxiety; Connectivity C1 [Roy, Amy] Fordham Univ, Dept Psychol, Bronx, NY 10458 USA. [Carlisi, Christina; Daniele, Teresa; Pine, Daniel; Ernst, Monique] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 551 BP 173S EP 174S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000546 ER PT J AU Blair, KS Teng, CY Geraci, M Blair, J Pine, DS AF Blair, Karina S. Teng, Cindy Y. Geraci, Marilla Blair, James Pine, Daniel S. TI A fMRI Investigation of Reduced Optimism and Reduced Optimistic Bias in Generalized Anxiety Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE generalized anxiety disorder; optimistic bias; fMRI; prefrontal cortex; optimism C1 [Blair, Karina S.; Teng, Cindy Y.; Geraci, Marilla; Blair, James; Pine, Daniel S.] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 553 BP 174S EP 174S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000548 ER PT J AU Vytal, KE Overstreet, C Robinson, O Grillon, C AF Vytal, Katherine E. Overstreet, Cassie Robinson, Oliver Grillon, Christian TI Exploring the Anxious Brain at Rest: Subcortico-Frontal Connectivity Changes Associated with an Anxious State SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Anxiety; Resting State; Connectivity C1 [Vytal, Katherine E.; Overstreet, Cassie; Robinson, Oliver; Grillon, Christian] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 554 BP 175S EP 175S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000549 ER PT J AU Ariza, VL Seguin, JR Nassim, M Rainville, P Boivin, M Pine, DS Lepore, F Tremblay, RE Maheu, F AF Ariza, Valerie La Buissonniere Seguin, Jean R. Nassim, Marouane Rainville, Pierre Boivin, Michel Pine, Daniel S. Lepore, Franco Tremblay, Richard E. Maheu, Francoise TI Harsh Parenting and Neural Fear Circuitry Function in High and Low Anxious Youths: Preliminary Findings SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE anxiety; harsh parenting; fMRI; fear circuitry; conditioning C1 [Ariza, Valerie La Buissonniere; Seguin, Jean R.; Rainville, Pierre; Lepore, Franco; Tremblay, Richard E.; Maheu, Francoise] Univ Montreal, Montreal, PQ, Canada. [Ariza, Valerie La Buissonniere; Seguin, Jean R.; Maheu, Francoise] CHU Ste Justine, Res Ctr, Montreal, PQ, Canada. [Boivin, Michel] Univ Laval, Quebec City, PQ, Canada. [Pine, Daniel S.] NIMH, Emot Dev & Affect Neurosci Sect, Bethesda, MD 20892 USA. RI Seguin, Jean/B-5349-2008; Boivin, Michel/J-3652-2013; Tremblay, Richard/O-1360-2014 OI Seguin, Jean/0000-0003-3359-6202; NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 564 BP 178S EP 178S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000559 ER PT J AU DeJong, K Kamath, T Chandramohan, D Kippenhan, S Roe, K Padmanabhan, A Kohn, P Mervis, C Pani, A Morris, C Weinberger, D Pierpaoli, C Marenco, S Berman, K AF DeJong, Katherine Kamath, Tushar Chandramohan, Dharshan Kippenhan, Shane Roe, Katherine Padmanabhan, Aarthi Kohn, Phillip Mervis, Carolyn Pani, Ariel Morris, Colleen Weinberger, Daniel Pierpaoli, Carlo Marenco, Stefano Berman, Karen TI Altered Tract Volume in Participants with Partial Deletions of the William Syndrome Chromosome Region SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Williams Syndrome; Diffusion Tensor Imaging; White matter C1 [DeJong, Katherine; Kamath, Tushar; Chandramohan, Dharshan; Marenco, Stefano] NIMH, Unit Multimodal Imaging Genet, CBDB, Bethesda, MD 20892 USA. [Kippenhan, Shane; Roe, Katherine; Padmanabhan, Aarthi; Kohn, Phillip; Berman, Karen] NIMH, Sect Integrat Neuroimaging, Bethesda, MD 20892 USA. [Mervis, Carolyn] Univ Louisville, Dept Psychol & Brain Sci, Neurodev Sci Lab, Louisville, KY 40292 USA. [Pani, Ariel] Stanford Univ, Dept Biol, Hopkins Marine Stn, Pacific Grove, CA USA. [Morris, Colleen] Univ Nevada, Sch Med, Dept Pediat, Div Genet, Las Vegas, NV 89154 USA. [Weinberger, Daniel] NIMH, GCAP CBDB, Bethesda, MD 20892 USA. [Pierpaoli, Carlo] NICHHD, Sect Tissue Biophys & Biomimet, Bethesda, MD 20892 USA. RI Marenco, Stefano/A-2409-2008 OI Marenco, Stefano/0000-0002-2488-2365 NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 573 BP 181S EP 181S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000568 ER PT J AU Gambale, CT Marenco, S Chandramohan, D Kippenhan, S Tost, H Lemaitre, H Kolachana, B Weinberger, DR Berman, KF AF Gambale, Catherine T. Marenco, Stefano Chandramohan, Dharshan Kippenhan, Shane Tost, Heike Lemaitre, Herve' Kolachana, Bhaskar Weinberger, Daniel R. Berman, Karen F. TI Association of a Single Nucleotide Polymorphism in LIMK1 with Diffusion Tensor Imaging (DTI) Measures SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE LIMK1; DTI; Williams; rs710969 C1 [Gambale, Catherine T.; Marenco, Stefano; Chandramohan, Dharshan; Tost, Heike; Lemaitre, Herve'; Kolachana, Bhaskar] NIMH, Clin Brain Disorders Branch Unit Multimodal Imagi, Bethesda, MD 20892 USA. [Kippenhan, Shane; Berman, Karen F.] NIMH, Sect Integrat Neuroimaging, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] NIMH, GCAP CBDB, NIMH IRP, Bethesda, MD 20892 USA. RI Marenco, Stefano/A-2409-2008 OI Marenco, Stefano/0000-0002-2488-2365 NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 574 BP 181S EP 181S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000569 ER PT J AU Lee, NR Raznahan, A Adeyemi, EI Hanley, A Clasen, LS Blumenthal, J Giedd, JN AF Lee, Nancy Raitano Raznahan, Armin Adeyemi, Elizabeth I. Hanley, Allison Clasen, Liv S. Blumenthal, Jonathan Giedd, Jay N. TI A Preliminary Investigation of Cortical Thickness and Surface Area in XYY: A Possible Genetic Model for Psychiatric Disorders Characterized by Social Cognitive Impairment? SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE sex chromosome; magnetic resonance imaging; XYY; cortical thickness; cortical surface area C1 [Lee, Nancy Raitano; Raznahan, Armin; Adeyemi, Elizabeth I.; Hanley, Allison; Clasen, Liv S.; Blumenthal, Jonathan; Giedd, Jay N.] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. RI Raznahan, Armin/F-4534-2012; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015; Lee, Nancy/M-7492-2016 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978; Lee, Nancy/0000-0002-6663-0713 NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 589 BP 186S EP 187S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000584 ER PT J AU Lee, MR Wu, WY Salmeron, BJ Gallen, C Yang, YH AF Lee, Mary R. Wu, Weiyue Salmeron, Betty Jo Gallen, Courtney Yang, Yihong TI The Effect of the COMT Val158Met Genotype and Early Life Stress on Brain Structure in Healthy Volunteers SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Childhood trauma; COMT; MRI; brain volume; insula C1 [Lee, Mary R.; Wu, Weiyue; Salmeron, Betty Jo; Gallen, Courtney; Yang, Yihong] Natl Inst Drug Abuse, Neuroimaging Res Branch, Baltimore, MD USA. RI Salmeron, Betty Jo/M-1793-2016 OI Salmeron, Betty Jo/0000-0003-1699-9333 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 590 BP 187S EP 187S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000585 ER PT J AU Raznahan, A Lenroot, R Thurm, A Gozzi, M Spence, S Giedd, JN Swedo, S AF Raznahan, Armin Lenroot, Rhoshel Thurm, Audrey Gozzi, Marta Spence, Sarah Giedd, Jay N. Swedo, Susan TI Compared to What? Head Circumference Overgrowth Relative to Available Population Norms does not Distinguish Infants with Autism from Typically Developing Controls SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Autism; Head Circumference; Development C1 [Raznahan, Armin; Lenroot, Rhoshel; Giedd, Jay N.] NIMH, Child Psychiat Branch, NIH, Bethesda, MD 20892 USA. [Thurm, Audrey; Gozzi, Marta; Swedo, Susan] NIMH, Pediat & Dev Neurosci Branch, NIH, Bethesda, MD 20892 USA. [Spence, Sarah] Harvard Univ, Sch Med, Dept Neurol, Boston, MA 02115 USA. RI Raznahan, Armin/F-4534-2012; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 592 BP 187S EP 187S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000587 ER PT J AU Wallace, GL Robustelli, B Dankner, N Kenworthy, L Giedd, JN Martin, A AF Wallace, Gregory L. Robustelli, Briana Dankner, Nathan Kenworthy, Lauren Giedd, Jay N. Martin, Alex TI Increased Cortical Surface Area and Gyrification During Adolescence and Young Adulthood in Autism Spectrum Disorders SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE autism; imaging; MRI; surface area; gyrification C1 [Wallace, Gregory L.; Robustelli, Briana; Dankner, Nathan; Kenworthy, Lauren; Giedd, Jay N.; Martin, Alex] NIMH, Lab Brain & Cognit, Bethesda, MD 20892 USA. RI martin, alex/B-6176-2009; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 591 BP 187S EP 187S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000586 ER PT J AU Noronha, CM Stockman, M White, R Lalonde, F Diwadkar, VA Gogtay, N AF Noronha, Carol M. Stockman, Michael White, Richard Lalonde, Francois Diwadkar, Vaibhav A. Gogtay, Nitin TI Aberrant Activation During Visually Guided Finger Tapping in Childhood Onset Schizophrenia: fMRI Evidence SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; Childhood-onset; fMRI; visuo-motor; activation C1 [Noronha, Carol M.; White, Richard; Diwadkar, Vaibhav A.] Wayne State Univ, Sch Med, Brain Res & Imaging Neurosci Div, Dept Psychiat & Behav Neurosci, Detroit, MI USA. [Stockman, Michael; Lalonde, Francois; Gogtay, Nitin] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 606 BP 192S EP 193S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000601 ER PT J AU Bloch, JB Wei, SM Bailer, EB Damme, KS Martinez, PE Kohn, PD Kippenhan, JS Rubinow, DR Schmidt, PJ Berman, KF AF Bloch, Jeffrey B. Wei, Shau-Ming Bailer, Erica B. Damme, Katherine S. Martinez, Pedro E. Kohn, Philip D. Kippenhan, Jonathan S. Rubinow, David R. Schmidt, Peter J. Berman, Karen F. TI Ovarian Hormones Differentially Modulate Subgenual Cingulate and Hippocampal Activity in Premenstrual Dysphoria at Rest - A Positron Emission Tomography (PET) Study SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Premenstrual Dysphoria (PMD); Gonadal Hormones; Mood Disorder; PET; Rest C1 [Bloch, Jeffrey B.; Wei, Shau-Ming; Bailer, Erica B.; Damme, Katherine S.; Kohn, Philip D.; Kippenhan, Jonathan S.; Berman, Karen F.] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Martinez, Pedro E.; Schmidt, Peter J.] NIMH, Sect Behav Endocrinol, Bethesda, MD 20892 USA. [Rubinow, David R.] Univ N Carolina, Sch Med, Dept Psychiat, Chapel Hill, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 629 BP 200S EP 201S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000624 ER PT J AU Adlemon, NE Razdan, V Walker, L Sarlls, J Pierpaoli, C Leibenluft, E AF Adlemon, Nancy E. Razdan, Varun Walker, Lindsay Sarlls, Joelle Pierpaoli, Carlo Leibenluft, Ellen TI White Matter Integrity in Children with Bipolar Disorder and Severe Mood Dysregulation SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE diffusion tensor imaging; bipolar disorder; severe mood dysregulation; pediatric; brain C1 [Adlemon, Nancy E.; Razdan, Varun; Leibenluft, Ellen] NIMH, NIH, Bethesda, MD 20892 USA. [Walker, Lindsay; Pierpaoli, Carlo] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD USA. [Sarlls, Joelle] NINDS, NIH MRI Res Facil, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 630 BP 201S EP 201S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000625 ER PT J AU Malek, M Watson, B AF Malek, Meaghan Watson, Bethany TI Cortical Change in New Onset Depression SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Depression; new onset; cortical change; at risk; cortical thickness C1 [Malek, Meaghan; Watson, Bethany] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 631 BP 201S EP 201S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466000626 ER PT J AU Insel, T AF Insel, Thomas TI New Frontiers: From Biological Psychiatry to Clinical Neuroscienc SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat ID HUMAN BRAIN C1 [Insel, Thomas] NIMH, Bethesda, MD 20892 USA. NR 3 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 682 BP 218S EP 218S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001005 ER PT J AU Berman, K AF Berman, Karen TI Linking Imaging Phenotypes in Williams Syndrome to Genetic Mechanisms SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Berman, Karen] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 687 BP 220S EP 220S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001010 ER PT J AU Lipska, BK AF Lipska, Barbara K. TI Postmortem Studies on Cortical Gene Expression in Williams Syndrome Patients and in Normal Development SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Lipska, Barbara K.] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 688 BP 220S EP 220S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001011 ER PT J AU Martin, A AF Martin, Alex TI Fractionating the Social Brain in Autism SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Martin, Alex] NIMH, Bethesda, MD 20892 USA. RI martin, alex/B-6176-2009 NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 689 BP 220S EP 220S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001012 ER PT J AU Barr, CS AF Barr, Christina S. TI Nonhuman Primate Models Afford Identification of Pathways and Critical Windows During which Genetic Factors Contribute to Risk for Stress-Psychiatric Disorders SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Barr, Christina S.] NIAAA, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 699 BP 223S EP 223S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001022 ER PT J AU Deveney, C AF Deveney, Christen TI Neural Correlates of Frustration Among Youths with Severe Mood Dysregulation SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Deveney, Christen] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 697 BP 223S EP 223S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001020 ER PT J AU Raznahan, A AF Raznahan, Armin TI The Importance of Journey over Destination for Psychiatric Neuroimaging SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Raznahan, Armin] NIMH, NIH, Bethesda, MD 20892 USA. RI Raznahan, Armin/F-4534-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 702B BP 225S EP 225S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001027 ER PT J AU Law, AJ AF Law, Amanda J. TI Genetic and Biological Epistasis Within the NRG1-ErbB4-PIK3CD Network. Context Dependant Regulation of AKT and a Novel Therapeutic Target SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE NRG1; ErbB4; Schizophrenia; AKT; human brain C1 [Law, Amanda J.] NIMH, NIH, Bethesda, MD 20892 USA. RI Law, Amanda/G-6372-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 706 BP 226S EP 226S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001031 ER PT J AU Weinberger, DR AF Weinberger, Daniel R. TI Epistatic Gene Networks in Hippocampal Plasticity and Schizophrenia Risk SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Weinberger, Daniel R.] NIMH, NIH, Inramural Res Program, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 705 BP 226S EP 226S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001030 ER PT J AU Bonci, A AF Bonci, Antonello TI Optogenetic Investigation of the Reward System SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Bonci, Antonello] NIDA, NIH, Intramural Res Program, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 708 BP 227S EP 227S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001033 ER PT J AU Cadet, JL AF Cadet, Jean Lud TI Chronic Methamphetamine Exposure Induces Large Scale Transcriptional Alterations and Epigenetic Modifications in the Rat Dorsal Striatum SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Cadet, Jean Lud] NIDA, NIH, Iintramural Res Program, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 709 BP 227S EP 227S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001034 ER PT J AU Brotman, MA AF Brotman, Melissa A. TI Neural Correlated of Face Emotion Processing and Cognitive Flexibility in Youth At Risk for Bipolar Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE bipolar disorder; at risk; face emotion; attention; fMRI C1 [Brotman, Melissa A.] NIMH, Bethesda, MD 20892 USA. RI Brotman, Melissa/H-7409-2013 NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 734 BP 233S EP 233S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001053 ER PT J AU Martinowich, K AF Martinowich, Keri TI Promoter-Specific Bdnf Transcription in Sleep-Related Plasticity SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Martinowich, Keri] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 750 BP 237S EP 237S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001069 ER PT J AU Greenstein, D AF Greenstein, Deanna TI Using Structural MRI to Explore Psychosis as a Dimension SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat C1 [Greenstein, Deanna] NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 760 BP 240S EP 240S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001079 ER PT J AU Alexander-Bloch, A Raznahan, A Lalonde, F Clasen, L Bullmore, ET Giedd, J AF Alexander-Bloch, Aaron Raznahan, Armin Lalonde, Francois Clasen, Liv Bullmore, Edward T. Giedd, Jay TI 6 Minutes of fMRI, 12 Years of Correlated Growth: A Comparison of Functional and Structural Large-Scale Brain Networks SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE graph theory; development; functional connectivity; cortical thickness; brain networks C1 [Alexander-Bloch, Aaron; Raznahan, Armin; Lalonde, Francois; Clasen, Liv; Giedd, Jay] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. [Alexander-Bloch, Aaron; Bullmore, Edward T.] Univ Cambridge, Cambridge, England. RI Raznahan, Armin/F-4534-2012; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015; Bullmore, Edward/C-1706-2012 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978; Bullmore, Edward/0000-0002-8955-8283 NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 EI 1873-2402 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 763 BP 241S EP 241S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001082 ER PT J AU Callicott, JH Zhang, FY Straub, RE Mattay, VS Weinberger, DR AF Callicott, Joseph H. Zhang, Fengyu Straub, Richard E. Mattay, Venkata S. Weinberger, Daniel R. TI Hippocampal Activation And Connectivity Effects Of A Genome-wide Schizophrenia Candidates Variant In Prr16 SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE fMRI; genetics; schizophrenia; hippocampus; genome-wide C1 [Callicott, Joseph H.; Zhang, Fengyu; Mattay, Venkata S.] NIMH, DIRP, NIH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Straub, Richard E.; Weinberger, Daniel R.] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 802 BP 253S EP 253S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001115 ER PT J AU Moran, ME Greenstein, DK Malley, JD Weisinger, BM Dasgupta, A Gogtay, N Rapoport, JL AF Moran, Marcel E. Greenstein, Deanna K. Malley, James D. Weisinger, Brian M. Dasgupta, Abhijit Gogtay, Nitin Rapoport, Judith L. TI Applying Multivariate Methods to Structural MRI Data using Childhood Onset Schizophrenia and Control Samples SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE MRI; Schizophrenia; Multivariate C1 [Moran, Marcel E.; Greenstein, Deanna K.; Weisinger, Brian M.; Gogtay, Nitin; Rapoport, Judith L.] NIMH, Child Psychiat Branch, NIH, Bethesda, MD 20892 USA. [Malley, James D.] NIH, Ctr Informat Technol, Bethesda, MD 20892 USA. [Dasgupta, Abhijit] NIAMS, Clin Trials & Outcomes Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 820 BP 259S EP 259S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001133 ER PT J AU Cropp, B Jabbi, M Kohn, P Baller, E Kippenhan, JS Eisenberg, D Masdeu, J Berman, KF AF Cropp, Brett Jabbi, Mbemba Kohn, Philip Baller, Erica Kippenhan, Jonathan S. Eisenberg, Daniel Masdeu, Joseph Berman, Karen F. TI Midbrain Presynaptic Dopamine Mediates Instrumental Choice Behavior SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Dopamine; Motivation; Midbrain; Decision Making; Instrumental Learning C1 [Cropp, Brett; Jabbi, Mbemba; Kohn, Philip; Baller, Erica; Kippenhan, Jonathan S.; Eisenberg, Daniel; Masdeu, Joseph; Berman, Karen F.] NIMH, Clin Brain Disorders Branch, Sect Integrated Neuroimaging, Bethesda, MD 20892 USA. RI Eisenberg, Daniel/C-7432-2014; Eisenberg, Daniel/S-4342-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 827 BP 262S EP 262S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001140 ER PT J AU Johnson, SL Greenstein, DK Lalonde, F Gogtay, N AF Johnson, Sarah L. Greenstein, Deanna K. Lalonde, Francois Gogtay, Nitin TI Corpus Callosal Volume in Childhood Onset Schizophrenia Patients, Healthy Siblings and Normal Volunteers SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE MRI; Schizophrenia; Corpus Callosum C1 [Johnson, Sarah L.; Greenstein, Deanna K.; Lalonde, Francois; Gogtay, Nitin] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 828 BP 262S EP 262S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001141 ER PT J AU Vyas, NS Rubinstein, DY Carver, FW Mattai, AA Stidd, R Holroyd, T Weisinger, BM David, CN Turner, C Clasen, L Miller, R Tossell, JW Coppola, R Rapoport, JL Gogtay, N AF Vyas, Nora S. Rubinstein, Daniel Y. Carver, Frederick W. Mattai, Anand A. Stidd, Reva Holroyd, Tom Weisinger, Brian M. David, Christopher N. Turner, Cheryl Clasen, Liv Miller, Rachel Tossell, Julia W. Coppola, Richard Rapoport, Judith L. Gogtay, Nitin TI Alterations in Magnetoencephalographic Gamma Oscillatory Patterns During Resting-State in Patients with Childhood-Onset Schizophrenia, Non-Psychotic Siblings and Healthy Controls SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE adolescent; schizophrenia; default network; MEG; synchrony C1 [Vyas, Nora S.; Mattai, Anand A.; Stidd, Reva; Weisinger, Brian M.; David, Christopher N.; Turner, Cheryl; Clasen, Liv; Miller, Rachel; Tossell, Julia W.; Rapoport, Judith L.; Gogtay, Nitin] NIH, Child Psychiat Branch, Bethesda, MD 20892 USA. [Rubinstein, Daniel Y.; Carver, Frederick W.; Holroyd, Tom; Coppola, Richard] NIH, MEG Core Facil, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 829 BP 262S EP 263S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001142 ER PT J AU Kaplan, CM Molina, JL Chen, Q Weinberger, DR Tan, HY AF Kaplan, Claire M. Molina, Juan L. Chen, Qiang Weinberger, Daniel R. Tan, Hao Yang TI Anterior and Dorsolateral Prefrontal Effective Connectivity Deficits in Evaluating and Updating Contextual Information in Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Schizophrenia; fMRI; Connectivity; Prefrontal; Decision C1 [Kaplan, Claire M.; Molina, Juan L.; Chen, Qiang; Weinberger, Daniel R.; Tan, Hao Yang] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 831 BP 263S EP 263S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001144 ER PT J AU Weisinger, B Greenstein, D Clasen, L Lalonde, F Miller, R Gochman, P Rapoport, JL Gogtay, N AF Weisinger, Brian Greenstein, Deanna Clasen, Liv Lalonde, Francois Miller, Rachel Gochman, Peter Rapoport, Judith L. Gogtay, Nitin TI Cortical Brain Development in Schizophrenia Spectrum Diagnosed Siblings of Patients with Childhood-Onset Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE schizophrenia; childhood-onset; siblings; spectrum; adolescents C1 [Weisinger, Brian; Greenstein, Deanna; Clasen, Liv; Lalonde, Francois; Miller, Rachel; Gochman, Peter; Rapoport, Judith L.; Gogtay, Nitin] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 830 BP 263S EP 263S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001143 ER PT J AU Xu, K Seo, D Hodgkinson, C Goldman, D Sinha, R AF Xu, Ke Seo, Dongju Hodgkinson, Colin Goldman, David Sinha, Rajita TI A Regulatory Variant in OPRK1 Modulated Hypothalamic and Limbic Brain Activity in Response to Stress and Predicts Relapse in Cocaine Dependence in African Americans SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE OPRK1; Brain activity; relapse; Cocaine dependence; African Americans C1 [Xu, Ke] Yale Univ, West Haven, CT USA. [Seo, Dongju; Sinha, Rajita] Yale Univ, Stress Ctr, New Haven, CT USA. [Hodgkinson, Colin; Goldman, David] NIAAA, Intramural Program, Rockville, MD 20852 USA. RI Seo, Dongju/F-2988-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 869 BP 275S EP 275S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001182 ER PT J AU Kawa, AB Hope, BT Harvey, BK Shaham, Y Calu, DJ AF Kawa, Alex B. Hope, Bruce T. Harvey, Brandon K. Shaham, Yavin Calu, Donna J. TI Effect of Optical Inhibition of Dorsal Medial Prefrontal Cortex Pyramidal Neurons on Yohimbine- and Pellet-Priming-Induced Reinstatement of Food Seeking in Female Rats SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE optogenetics; reinstatement; feeding; dorsal medial prefrontal cortex; stress C1 [Kawa, Alex B.; Hope, Bruce T.; Harvey, Brandon K.; Shaham, Yavin; Calu, Donna J.] NIDA, Baltimore, MD USA. RI Hope, Bruce/A-9223-2010 OI Hope, Bruce/0000-0001-5804-7061 NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 897 BP 284S EP 284S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001210 ER PT J AU Trampush, JW Weinberger, D Dickinson, D AF Trampush, Joey W. Weinberger, Daniel Dickinson, Dwight TI Scales for Investigation of Normal and Pathological Personality Traits SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE personality; schizophrenia; negative affect; positive affect; psychopathology C1 [Trampush, Joey W.; Weinberger, Daniel; Dickinson, Dwight] NIMH, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 904 BP 286S EP 286S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001217 ER PT J AU Fanous, S Guez-Barber, D Goldart, EM Schrama, R Theberge, F Bossert, JM Van Seters, S Shoham, Y Hope, BT AF Fanous, Sanya Guez-Barber, Danielle Goldart, Evan M. Schrama, Regina Theberge, Florence Bossert, Jennifer M. Van Seters, Sebastiaan Shoham, Yavin Hope, Bruce T. TI Characterization of Prefrontal Cortex Neuronal Ensembles in Cue-induced Heroin Seeking Using Facs and Neural Inactivation SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE self-administration; flow cytometry; opiate; neuronal ensemble; relapse C1 [Fanous, Sanya; Guez-Barber, Danielle; Goldart, Evan M.; Schrama, Regina; Theberge, Florence; Bossert, Jennifer M.; Van Seters, Sebastiaan; Shoham, Yavin; Hope, Bruce T.] NIDA, Baltimore, MD USA. [Guez-Barber, Danielle] Yale Univ, New Haven, CT USA. RI Hope, Bruce/A-9223-2010 OI Hope, Bruce/0000-0001-5804-7061 NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 909 BP 287S EP 288S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001222 ER PT J AU Gorelick, DA AF Gorelick, David A. TI fMRI-Guided Transcranial Magnetic Stimulation (TMS) to Reduce Cue-Induced Nicotine Craving SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE transcranial magnetic stimulation; fMRI; cue-induced; craving; nicotine C1 [Gorelick, David A.] NIDA, IRP, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 911 BP 288S EP 288S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001224 ER PT J AU Robinson, OJ Allen, P Overstreet, C Pine, DS Grillon, C AF Robinson, Oliver J. Allen, Phillip Overstreet, Cassie Pine, Daniel S. Grillon, Christian TI Acute Tryptophan Depletion Increases Translational Indices of Anxiety but Not Fear: Implications for Mood and Anxiety Disorders SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Serotonin; anxiety; startle responses; CRH; BNST C1 [Robinson, Oliver J.; Allen, Phillip; Overstreet, Cassie; Pine, Daniel S.; Grillon, Christian] NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 913 BP 289S EP 289S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001226 ER PT J AU Marquardt, CA Ibrahim, L Luckenbaugh, DA Zarate, CA AF Marquardt, Craig A. Ibrahim, Lobna Luckenbaugh, David A. Zarate, Carlos A. TI Family History of Alcohol Dependence and Initial Antidepressant Response to an N-methyl-D-aspartate Antagonist in Bipolar Depression SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Ketamine; Bipolar disorder; Alcohol dependence; Family history; Anitdepressant response C1 [Marquardt, Craig A.; Ibrahim, Lobna; Luckenbaugh, David A.; Zarate, Carlos A.] NIMH, ETPB, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 924 BP 292S EP 292S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001237 ER PT J AU Selter, JH Duncan, WC Sarasso, S Zarate, CA AF Selter, Jessica H. Duncan, Wallace C. Sarasso, Simone Zarate, Carlos A. TI Delta Sleep Ratio Predicts Ketamine Response in Major Depressive Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Ketamine; Mood Disorder; Delta Sleep C1 [Selter, Jessica H.; Duncan, Wallace C.; Zarate, Carlos A.] NIMH, Expt Therapeut & Pathophysiol Branch, Bethesda, MD 20892 USA. [Sarasso, Simone] Univ Wisconsin, Dept Psychiat, Madison, WI 53706 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 925 BP 292S EP 292S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001238 ER PT J AU Furey, M Drevets, W Khanna, A Zarate, C AF Furey, Maura Drevets, Wayne Khanna, Ashish Zarate, Carlos TI Differences in Response to Implicit Emotional Faces in Anterior Cingulate Cortex Predict Antidepressant Response to Scopolamine in Major Depressive Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE biomarker of response; antimuscarinic; neuroimaging; cognitive neuroscience; emotion processing bias C1 [Furey, Maura; Zarate, Carlos] NIMH, Mood & Anxiety Disorders Program, NIH, Bethesda, MD 20892 USA. [Drevets, Wayne] Univ Oklahoma, Coll Med, Dept Psychiat, Laureate Inst Brain Res, Norman, OK 73019 USA. [Khanna, Ashish] Amer Univ Caribbean, Sch Med, Coral Gables, FL USA. RI Furey, Maura/H-5273-2013 NR 0 TC 1 Z9 1 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 928 BP 293S EP 293S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001241 ER PT J AU Szczepanik, J Drevets, W Khanna, A Zarate, C Furey, M AF Szczepanik, Joanna Drevets, Wayne Khanna, Ashish Zarate, Carlos Furey, Maura TI Amygdala Response to Implicit and Explicit Sad Face Stimuli at Baseline Predicts Antidepressant Treatment Response to Scopolamine in Major Depressive Disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Cognitive Neuroscience; Emotion; Fmri; Biomarkers; Antimuscarinic C1 [Szczepanik, Joanna; Zarate, Carlos; Furey, Maura] NIMH, Bethesda, MD 20892 USA. [Szczepanik, Joanna] Univ Maryland, College Pk, MD 20742 USA. [Drevets, Wayne] Laureate Inst Brain Res, Tulsa, OK USA. [Khanna, Ashish] Amer Univ Caribbean, Sch Med, Coral Gables, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 927 BP 293S EP 293S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001240 ER PT J AU Theberge, F Kambhampati, S Eichenbaum, H Pickens, C Rice, K Shaham, Y AF Theberge, Florence Kambhampati, Santa Eichenbaum, Hila Pickens, Charles Rice, Kenner Shaham, Yavin TI Effects of the Preferential Mu Opioid Receptor Antagonist(-)- Naloxone and the Toll-like Receptor 4 Antagonist (+)- Naloxone on Heroin Self-Administration and Incubation of Heroin Craving SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Heroin; self-administration; relapse; naloxone; toll-like receptor C1 [Theberge, Florence; Kambhampati, Santa; Eichenbaum, Hila; Pickens, Charles; Rice, Kenner; Shaham, Yavin] NIDA, NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 962 BP 303S EP 304S PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001274 ER PT J AU Fitzgerald, PJ Whittle, N Flynn, SM Singewald, N Holmes, A AF Fitzgerald, Paul J. Whittle, Nigel Flynn, Shaun M. Singewald, Nicolas Holmes, Andrew TI Fluoxetine Modulates Infralimbic Neuronal Extinction-Encoding in a Mouse Model of Persistent Fear SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE fluoxetine; fear conditioning; neuropharmacology; inbred mouse strain; ptsd C1 [Fitzgerald, Paul J.; Flynn, Shaun M.; Holmes, Andrew] NIH, Lab Behav & Genom Neurosci, Rockville, MD USA. [Whittle, Nigel; Singewald, Nicolas] Univ Innsbruck, Dept Pharmacol & Toxicol, A-6020 Innsbruck, Austria. NR 0 TC 0 Z9 0 U1 2 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 976 BP 307S EP 307S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001287 ER PT J AU Jarcho, JM Fox, NA Pine, DS Leibenluft, E Shechner, T Ernst, M AF Jarcho, Johanna M. Fox, Nathan A. Pine, Daniel S. Leibenluft, Ellen Shechner, Tomer Ernst, Monique TI Neural Response to Emotional Conflict and Conflict Resolution is Altered Among Young Adults Characterized as Behaviorally Inhibited During Early Childhood SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Behavioral Inhibition; Conflict Adaptation; Emotional Conflict; fMRI C1 [Jarcho, Johanna M.; Pine, Daniel S.; Shechner, Tomer; Ernst, Monique] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. [Fox, Nathan A.] Univ Maryland, Inst Child Study, Dept Human Dev, College Pk, MD 20742 USA. [Leibenluft, Ellen] NIMH, Sect Bipolar Spectrum Disorders, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 975 BP 307S EP 307S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001286 ER PT J AU Cho, YT Ernst, M Fudge, JL AF Cho, Youngsun T. Ernst, Monique Fudge, Julie L. TI The Functional Stations of the Amygdala, Based on Cortico-Amygdala-Striatal Circuits SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Amygdala; Prefrontal Cortex; Striatum; Insula; Circuits C1 [Cho, Youngsun T.; Fudge, Julie L.] Univ Rochester, Sch Med & Dent, Rochester, NY USA. [Ernst, Monique] NIMH, Sect Dev & Affect Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 978 BP 308S EP 308S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001289 ER PT J AU Molina, JL Kaplan, CM Chen, Q Weinberger, DR Tan, HY AF Molina, Juan L. Kaplan, Claire M. Chen, Qiang Weinberger, Daniel R. Tan, Hao Yang TI Specific Cortical-Striatal Connectivity Deficits in Working Memory Encoding in Schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE Effective connectivity; Dynamic causal modeling; Schizophrenia; Executive function C1 [Molina, Juan L.; Kaplan, Claire M.; Chen, Qiang; Weinberger, Daniel R.; Tan, Hao Yang] NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Weinberger, Daniel R.] Johns Hopkins Univ, Leiber Inst Brain Dev, Med Ctr, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 996 BP 313S EP 313S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001307 ER PT J AU Tan, HY Chen, AG Kolachana, B Apud, JA Mattay, VS Callicott, JH Chen, Q Weinberger, DR AF Tan, Hao Yang Chen, Anthony G. Kolachana, Bhaskar Apud, Jose A. Mattay, Venkata S. Callicott, Joseph H. Chen, Qiang Weinberger, Daniel R. TI Effective Connectivity of Akt1-Mediated Dopaminergic Working Memory Networks and the Pharmacogenetics of Antidopaminergic Treatment SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract CT 67th Annual Scientific Convention and Meeting of the Society-of-Biological-Psychiatry CY MAY 03-05, 2012 CL Philadelphia, PA SP Soc Biol Psychiat DE COMT; DRD2; antipsychotics; fMRI; IQ C1 [Tan, Hao Yang; Chen, Anthony G.; Kolachana, Bhaskar; Apud, Jose A.; Mattay, Venkata S.; Callicott, Joseph H.; Chen, Qiang; Weinberger, Daniel R.] NIH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. [Chen, Qiang; Weinberger, Daniel R.] Lieber Inst Brain Dev, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 2012 VL 71 IS 8 SU S MA 997 BP 314S EP 314S PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 921GL UT WOS:000302466001308 ER PT J AU Rajan, A Carter, CA Kelly, RJ Gutierrez, M Kummar, S Szabo, E Yancey, MA Ji, JP Mannargudi, B Woo, S Spencer, S Figg, WD Giaccone, G AF Rajan, Arun Carter, Corey A. Kelly, Ronan J. Gutierrez, Martin Kummar, Shivaani Szabo, Eva Yancey, Mary Ann Ji, Jiuping Mannargudi, Baskar Woo, Sukyung Spencer, Shawn Figg, William Douglas Giaccone, Giuseppe TI A Phase I Combination Study of Olaparib with Cisplatin and Gemcitabine in Adults with Solid Tumors SO CLINICAL CANCER RESEARCH LA English DT Article ID RIBOSE POLYMERASE INHIBITOR; POLY(ADP-RIBOSE) POLYMERASE-1; REPAIR; CANCER; CELLS; TEMOZOLOMIDE; DEFICIENT; THERAPY; PATHWAY AB Purpose: To determine the safety and tolerability of olaparib with cisplatin and gemcitabine, establish the maximum tolerated dose (MTD), and evaluate the pharmacodynamic and pharmacokinetic profile of the combination. Experimental Design: We conducted a phase I study of olaparib with cisplatin and gemcitabine in patients with advanced solid tumors. Treatment at dose level 1 (DL1) consisted of olaparib 100 mg orally every 12 hours on days 1 to 4, gemcitabine 500 mg/m(2) on days 3 and 10, and cisplatin 60 mg/m(2) on day 3. PAR levels were measured in peripheral blood mononuclear cells (PBMC). Results: Dose-limiting toxicities (DLT) in two of three patients at DL1 included thrombocytopenia and febrile neutropenia. The protocol was amended to enroll patients treated with <= 2 prior severely myelo-suppressive chemotherapy regimens and treated with olaparib 100 mg once daily on days 1 to 4 (DL-1). No DLTs were seen in six patients at DL-1. Because of persistent thrombocytopenia and neutropenia following a return to DL1, patients received 100 mg olaparib every 12 hours on day 1 only. No hematologic DLTs were observed; nonhematologic DLTs included gastrointestinal bleed, syncope, and hypoxia. Of 21 patients evaluable for response, two had partial response. Olaparib inhibited PARP in PBMCs and tumor tissue, although PAR levels were less effectively inhibited when olaparib was used for a short duration. Conclusions: Olaparib in combination with cisplatin and gemcitabine is associated with myelosuppression even at relatively low doses. Modified schedules of olaparib in chemotherapy naive patients will have to be explored with standard doses of chemotherapy. Clin Cancer Res; 18(8); 2344-51. (C) 2012 AACR. C1 [Rajan, Arun; Carter, Corey A.; Kelly, Ronan J.; Gutierrez, Martin; Kummar, Shivaani; Szabo, Eva; Yancey, Mary Ann; Ji, Jiuping; Mannargudi, Baskar; Woo, Sukyung; Figg, William Douglas; Giaccone, Giuseppe] NCI, Med Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Spencer, Shawn] NCI, Clin Pharmacol Program, SAIC Frederick Inc, Ctr Canc Res, Frederick, MD USA. RP Giaccone, G (reprint author), NCI, Med Oncol Branch, Ctr Canc Res, NIH, 10 Ctr Dr,Room 12N226, Bethesda, MD 20892 USA. EM giacconeg@mail.nih.gov RI Figg Sr, William/M-2411-2016; Giaccone, Giuseppe/E-8297-2017; OI Giaccone, Giuseppe/0000-0002-5023-7562; Mannargudi, Baskar/0000-0001-6430-0641 FU National Cancer Institute, NIH; AstraZeneca; Center for Cancer Research, National Cancer Institute, NIH FX This project has been funded in whole or in part with federal funds from the National Cancer Institute, NIH. This work was supported by AstraZeneca and the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, NIH. NR 23 TC 63 Z9 65 U1 0 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 J9 CLIN CANCER RES JI Clin. Cancer Res. PD APR 15 PY 2012 VL 18 IS 8 BP 2344 EP 2351 DI 10.1158/1078-0432.CCR-11-2425 PG 8 WC Oncology SC Oncology GA 927KW UT WOS:000302907300026 PM 22371451 ER PT J AU Stein, WD Wilkerson, J Kim, ST Huang, X Motzer, RJ Fojo, AT Bates, SE AF Stein, Wilfred D. Wilkerson, Julia Kim, Sindy T. Huang, Xin Motzer, Robert J. Fojo, Antonio Tito Bates, Susan E. TI Analyzing the Pivotal Trial That Compared Sunitinib and IFN-alpha in Renal Cell Carcinoma, Using a Method That Assesses Tumor Regression and Growth SO CLINICAL CANCER RESEARCH LA English DT Article ID PHASE-III TRIAL; INTERFERON-ALPHA; CLINICAL-TRIAL; RATE CONSTANTS; DOUBLE-BLIND; SURVIVAL; BEVACIZUMAB AB Purpose: We applied a method that analyzes tumor response, quantifying the rates of tumor growth (g) and regression (d), using tumor measurements obtained while patients receive therapy. We used data from the phase III trial comparing sunitinib and IFN-alpha in metastatic renal cell carcinoma (mRCC) patients. Methods: The analysis used an equation that extracts d and g. Results: For sunitinib, overall survival (OS) was strongly correlated with log g (Rsq = 0.44, P < 0.0001); much less with log d (Rsq = 0.04; P = 0.0002). The median g of tumors in these patients (0.00082 per days; log g = 3.09) was about half that (P < 0.001) of tumors in patients receiving IFN-alpha (0.0015 per day; log g = = 2.81). With IFN-alpha, the OS/log g correlation (Rsq = 0.14) was weaker. Values of g from measurements obtained by study investigators or central review were highly correlated (Rsq = 0.80). No advantage resulted in including data from central review in regressions. Furthermore, g can be estimated accurately four months before treatment discontinuation. Extrapolating g in a model that incorporates survival generates the hypothesis that g increased after discontinuation of sunitinib but did not accelerate. Conclusions: In patients with mRCC, sunitinib reduced tumor growth rate, g, more than did IFN-alpha. Correlating g with OS confirms earlier analyses suggesting g may be an important clinical trial endpoint, to be explored prospectively and in individual patients. Clin Cancer Res; 18(8); 2374-81. (C) 2012 AACR. C1 [Stein, Wilfred D.; Wilkerson, Julia; Fojo, Antonio Tito; Bates, Susan E.] NCI, Med Oncol Branch, NIH, Bethesda, MD 20892 USA. [Stein, Wilfred D.] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Jerusalem, Israel. [Kim, Sindy T.; Huang, Xin] Pfizer Inc, La Jolla, CA USA. [Motzer, Robert J.] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. RP Bates, SE (reprint author), NCI, Med Oncol Branch, NIH, Bldg 10,RM 12N226,9000 Rockville Pike, Bethesda, MD 20892 USA. EM sebates@helix.nih.gov FU NIH; Pfizer FX This work was supported by NIH Intramural Research Program.; S.T. Kim: stock, Pfizer. R.J. Motzer received commercial research support and is a consultant on the advisory board of Pfizer. No other potential conflicts of interest were disclosed by the other authors. NR 21 TC 27 Z9 28 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 J9 CLIN CANCER RES JI Clin. Cancer Res. PD APR 15 PY 2012 VL 18 IS 8 BP 2374 EP 2381 DI 10.1158/1078-0432.CCR-11-2275 PG 8 WC Oncology SC Oncology GA 927KW UT WOS:000302907300029 PM 22344231 ER PT J AU Jovic, M Kean, MJ Szentpetery, Z Polevoy, G Gingras, AC Brill, JA Balla, T AF Jovic, Marko Kean, Michelle J. Szentpetery, Zsofia Polevoy, Gordon Gingras, Anne-Claude Brill, Julie A. Balla, Tamas TI Two phosphatidylinositol 4-kinases control lysosomal delivery of the Gaucher disease enzyme, beta-glucocerebrosidase SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID OXYSTEROL-BINDING-PROTEIN; GOLGI-COMPLEX; PLASMA-MEMBRANE; LIMP-II; SACCHAROMYCES-CEREVISIAE; INTRACELLULAR-TRANSPORT; ENDOPLASMIC-RETICULUM; MASS-SPECTROMETRY; CYTOPLASMIC TAIL; CONTACT SITES AB Gaucher disease is a lysosomal storage disorder caused by a defect in the degradation of glucosylceramide catalyzed by the lysosomal enzyme beta-glucocerebrosidase (GBA). GBA reaches lysosomes via association with its receptor, lysosomal integral membrane protein type 2 (LIMP-2). We found that distinct phosphatidylinositol 4-kinases (PI4Ks) play important roles at multiple steps in the trafficking pathway of the LIMP-2/GBA complex. Acute depletion of phosphatidylinositol 4-phosphate in the Golgi caused accumulation of LIMP-2 in this compartment, and PI4KIII beta was found to be responsible for controlling the exit of LIMP-2 from the Golgi. In contrast, depletion of PI4KII alpha blocked trafficking at a post-Golgi compartment, leading to accumulation of LIMP-2 in enlarged endosomal vesicles. PI4KII alpha depletion also caused secretion of missorted GBA into the medium, which was attenuated by limiting LIMP-2/GBA exit from the Golgi by PI4KIII beta inhibitors. These studies identified PI4KIII beta and PI4KII alpha as important regulators of lysosomal delivery of GBA, revealing a new element of control to sphingolipid homeostasis by phosphoinositides. C1 [Jovic, Marko; Szentpetery, Zsofia; Balla, Tamas] NICHHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD 20892 USA. [Kean, Michelle J.; Gingras, Anne-Claude] Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada. [Kean, Michelle J.; Gingras, Anne-Claude; Brill, Julie A.] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada. [Polevoy, Gordon; Brill, Julie A.] Hosp Sick Children, Cell Biol Program, Toronto, ON M5G 1L7, Canada. RP Balla, T (reprint author), NICHHD, Sect Mol Signal Transduct, Program Dev Neurosci, NIH, Bethesda, MD 20892 USA. EM ballat@mail.nih.gov RI Gingras, Anne-Claude/E-9982-2010; OI Gingras, Anne-Claude/0000-0002-6090-4437; Balla, Tamas/0000-0002-9077-3335 FU Eunice Kennedy Shriver NICHD, NIH; Canadian Institutes of Health Research (CIHR) [MOP-84314, CIHR MOP-102546] FX Confocal imaging was performed at the Microscopy and Imaging Core of the NICHD, NIH, with the kind assistance of Vincent Schram and James T. Russell. We thank Jason Burgess for reading the manuscript. This research was supported in part by the Intramural Research Program of the Eunice Kennedy Shriver NICHD, NIH. Work in the Gingras and Brill laboratories was supported by the Canadian Institutes of Health Research (CIHR MOP-84314 to A.-C.G. and CIHR MOP-102546 to J.A.B.). A.-C.G. holds the Lea Reichmann Chair in Cancer Proteomics and a Canada Research Chair in Functional Proteomics. M.J.K. is supported by CIHR through a Banting and Best Canada Graduate Scholarship. NR 65 TC 39 Z9 39 U1 0 U2 5 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR 15 PY 2012 VL 23 IS 8 BP 1533 EP 1545 DI 10.1091/mbc.E11-06-0553 PG 13 WC Cell Biology SC Cell Biology GA 930BX UT WOS:000303111900014 PM 22337770 ER PT J AU Lin, S Yang, JH Elkahloun, AG Bandyopadhyay, A Wang, L Cornell, JE Yeh, IT Agyin, J Tomlinson, G Sun, LZ AF Lin, Shu Yang, Junhua Elkahloun, Abdel G. Bandyopadhyay, Abhik Wang, Long Cornell, John E. Yeh, I-Tien Agyin, Joseph Tomlinson, Gail Sun, Lu-Zhe TI Attenuation of TGF-beta signaling suppresses premature senescence in a p21-dependent manner and promotes oncogenic Ras-mediated metastatic transformation in human mammary epithelial cells SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID GROWTH-FACTOR-BETA; CANCER MDA-MB-231 CELLS; BREAST-CANCER; GENE-EXPRESSION; C-MYC; II RECEPTOR; INHIBITION; KERATINOCYTES; TUMORIGENESIS; CARCINOMA AB The molecular mechanisms that drive triple-negative, basal-like breast cancer progression are elusive. Few molecular targets have been identified for the prevention or treatment of this disease. Here we developed a series of isogenic basal-like human mammary epithelial cells (HMECs) with altered transforming growth factor-beta (TGF-beta) sensitivity and different malignancy, resembling a full spectrum of basal-like breast carcinogenesis, and determined the molecular mechanisms that contribute to oncogene-induced transformation of basal-like HMECs when TGF-beta signaling is attenuated. We found that expression of a dominant-negative type II receptor (DNRII) of TGF-beta abrogated autocrine TGF-beta signaling in telomerase-immortalized HMECs and suppressed H-Ras-V12-induced senescence-like growth arrest (SLGA). Furthermore, coexpression of DNRII and H-Ras-V12 rendered HMECs highly tumorigenic and metastatic in vivo in comparison with H-Ras-V12-transformed HMECs that spontaneously escaped H-Ras-V12-induced SLGA. Microarray analysis revealed that p21 was the major player mediating Ras-induced SLGA, and attenuated or loss of p21 expression contributed to the escape from SLGA when autocrine TGF-beta signaling was blocked in HMECs. Furthermore, knockdown of p21 also suppressed H-Ras-V12-induced SLGA. Our results identify that autocrine TGF-beta signaling is an integral part of the cellular anti-transformation network by suppressing the expression of a host of genes, including p21-regulated genes, that mediate oncogene-induced transformation in basal-like breast cancer. C1 [Lin, Shu; Yang, Junhua; Bandyopadhyay, Abhik; Wang, Long; Agyin, Joseph; Sun, Lu-Zhe] Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA. [Elkahloun, Abdel G.] NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. [Cornell, John E.] Univ Texas Hlth Sci Ctr San Antonio, Dept Epidemiol & Biostat, San Antonio, TX 78229 USA. [Yeh, I-Tien] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA. [Tomlinson, Gail] Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, San Antonio, TX 78229 USA. [Sun, Lu-Zhe] Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA. RP Sun, LZ (reprint author), Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA. EM sunl@uthscsa.edu RI Lin, Shu/C-3790-2013 FU National Institutes of Health [R01CA75253, R01CA79683]; Cancer Therapy and Research Center at the University of Texas Health Science Center at San Antonio (UTHSCSA) through National Cancer Institute Cancer Center [2 P30 CA054174-17] FX This work was supported in part by National Institutes of Health Grants R01CA75253 and R01CA79683 and the Cancer Therapy and Research Center at the University of Texas Health Science Center at San Antonio (UTHSCSA) through National Cancer Institute Cancer Center Support Grant 2 P30 CA054174-17. We thank Andrew Hinck (UTHSCSA) for the recombinant TGF-beta 3, Peter J. Hornsby (UTHSCSA) for the H-Ras-V12 expression plasmid, Xinhua Feng (Baylor College of Medicine, Houston, TX) for the p21 promoter-Luc report plasmid, Gokul Das (Roswell Park Cancer Institute, Buffalo, NY) for the PCNA promoter-Luc report plasmid, and Bert Vogelstein (Johns Hopkins Medical School) for the pSBE4-Luc plasmid. We also thank James Jackson (MD Anderson Cancer Center, Houston, TX) for technical assistance with the chromatin immunoprecipitation assay. NR 64 TC 21 Z9 21 U1 0 U2 6 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR 15 PY 2012 VL 23 IS 8 BP 1569 EP 1581 DI 10.1091/mbc.E11-10-0849 PG 13 WC Cell Biology SC Cell Biology GA 930BX UT WOS:000303111900017 PM 22357622 ER PT J AU Stubblefield, MD McNeely, ML Alfano, CM Mayer, DK AF Stubblefield, Michael D. McNeely, Margaret L. Alfano, Catherine M. Mayer, Deborah K. TI A prospective surveillance model for physical rehabilitation of women with breast cancer SO CANCER LA English DT Article DE cancer; rehabilitation; survivorship; neuropathy; chemotherapy; radiculopathy; brachial plexopathy; breast cancer; surveillance; pain; palliative care ID INDUCED PERIPHERAL NEUROPATHY; PHASE-I TRIAL; CHARCOT-MARIE-TOOTH; LONG-TERM-CARE; OVARIAN-CANCER; CHEMOTHERAPY; TAXOL; MANAGEMENT; SURVIVORS; EXERCISE AB Chemotherapy-induced peripheral neuropathy (CIPN) results from damage to or dysfunction of the peripheral nerves. The development of CIPN is anticipated for the majority of breast cancer patients who receive neurotoxic chemotherapy, depending on the agent used, dose, and schedule. Sensory symptoms often predominate and include numbness, tingling, and distal extremity pain. Weakness, gait impairment, loss of functional abilities, and other deficits may develop with more severe CIPN. This article outlines a prospective surveillance model for physical rehabilitation of women with breast cancer who develop CIPN. Rehabilitative efforts for CIPN start at the time of breast cancer diagnosis and treatment planning. The prechemotherapy evaluation identifies patients with preexisting peripheral nervous system disorders that may place them at higher risk for the development of CIPN. This clinical evaluation should include a history focusing on symptoms and functional activities as well as a physical examination that objectively assesses the patient's strength, sensation, reflexes, and gait. Ongoing surveillance following the initiation of a neurotoxic agent is important to monitor for the development and progression of symptoms associated with CIPN, and to ensure its resolution over the long term. CIPN is managed best by a multidisciplinary team approach. Early identification of symptoms will ensure appropriate referral and timely symptom management. The prospective surveillance model promotes a patient-centered approach to care, from pretreatment through survivorship and palliative care. In this way, the model offers promise in addressing and minimizing both the acute and long-term morbidity associated with CIPN. Cancer 2012;. (c) 2012 American Cancer Society. C1 [Stubblefield, Michael D.] Mem Sloan Kettering Canc Ctr, Sillerman Ctr Rehabil, New York, NY 10022 USA. [Stubblefield, Michael D.] Mem Sloan Kettering Canc Ctr, Dept Neurol, Rehabil Med Serv, New York, NY 10022 USA. [Stubblefield, Michael D.] Cornell Univ, Weill Med Coll, Dept Phys Med & Rehabil, New York, NY 10021 USA. [McNeely, Margaret L.] Univ Alberta, Dept Phys Therapy & Oncol, Edmonton, AB, Canada. [McNeely, Margaret L.] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada. [Alfano, Catherine M.] NCI, Off Canc Survivorship, Div Canc Control & Populat Sci, NIH, Bethesda, MD 20892 USA. [Mayer, Deborah K.] Univ N Carolina, Sch Nursing, Chapel Hill, NC USA. RP Stubblefield, MD (reprint author), Mem Sloan Kettering Canc Ctr, Sillerman Ctr Rehabil, 515 Madison Ave,5th Floor, New York, NY 10022 USA. EM stubblem@mskcc.org FU American Cancer Society through The Longaberger Company(R); Longaberger Horizon of Hope(R) Campaign FX Support for this meeting and supplement were provided by the American Cancer Society through The Longaberger Company (R), a direct selling company offering home products including hand-crafted baskets made in Ohio, and the Longaberger Horizon of Hope (R) Campaign, which provided a grant to the American Cancer Society for breast cancer research and education. NR 63 TC 26 Z9 27 U1 1 U2 7 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0008-543X J9 CANCER-AM CANCER SOC JI Cancer PD APR 15 PY 2012 VL 118 SU 8 BP 2250 EP 2260 DI 10.1002/cncr.27463 PG 11 WC Oncology SC Oncology GA 922JV UT WOS:000302544100008 PM 22488699 ER PT J AU Sharma, A Chen, QH Nguyen, T Yu, Q Sen, JM AF Sharma, Archna Chen, Qinghua Trang Nguyen Yu, Qing Sen, Jyoti Misra TI T Cell Factor-1 and beta-Catenin Control the Development of Memory-like CD8 Thymocytes SO JOURNAL OF IMMUNOLOGY LA English DT Article ID DEFICIENT MICE; PATHWAY; TCF-1; DIFFERENTIATION; EXPRESSION; GENERATION; SELECTION; LINEAGE AB Innate memory-like CD8 thymocytes develop and acquire effector function during maturation in the absence of encounter with Ags. In this study, we demonstrate that enhanced function of transcription factors T cell factor (TCF)-1 and beta-catenin regulate the frequency of promyelocytic leukemia zinc finger (PLZF)-expressing, IL-4-producing thymocytes that promote the generation of eomesodermin-expressing memory-like CD8 thymocytes in trans. In contrast, TCF1-deficient mice do not have PLZF-expressing thymocytes and eomesodermin-expressing memory-like CD8 thymocytes. Generation of TCF1 and beta-catenin-dependent memory-like CD8 thymocytes is non-cell-intrinsic and requires the expression of IL-4 and IL-4R. CD8 memory-like thymocytes migrate to the peripheral lymphoid organs, and the memory-like CD8 T cells rapidly produce IFN-gamma. Thus, TCF1 and beta-catenin regulate the generation of PLZF-expressing thymocytes and thereby facilitate the generation of memory-like CD8 T cells in the thymus. The Journal of Immunology, 2012, 188: 3859-3868. C1 [Sharma, Archna; Chen, Qinghua; Trang Nguyen; Yu, Qing; Sen, Jyoti Misra] NIA, Lab Mol Biol & Immunol, NIH, Baltimore, MD 21224 USA. RP Sen, JM (reprint author), NIA, Lab Mol Biol & Immunol, NIH, 8C218 Biomed Res Ctr Bldg,251 Bayview Blvd, Baltimore, MD 21224 USA. EM Jyoti-Sen@nih.gov RI Sharma, Archna/R-9377-2016 OI Sharma, Archna/0000-0003-4745-0220 FU National Institute on Aging at the National Institutes of Health FX This work was supported by the Intramural Research Program of the National Institute on Aging at the National Institutes of Health. NR 35 TC 17 Z9 17 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 2012 VL 188 IS 8 BP 3859 EP 3868 DI 10.4049/jimmunol.1103729 PG 10 WC Immunology SC Immunology GA 923TD UT WOS:000302641400033 PM 22492686 ER PT J AU Ranguelova, K Rice, AB Khajo, A Triquigneaux, M Garantziotis, S Magliozzo, RS Mason, RP AF Ranguelova, Kalina Rice, Annette B. Khajo, Abdelahad Triquigneaux, Mathilde Garantziotis, Stavros Magliozzo, Richard S. Mason, Ronald P. TI Formation of reactive sulfite-derived free radicals by the activation of human neutrophils: An ESR study SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE Sulfite toxicity; Sulfite-derived radicals; ESR spectroscopy; DMPO; Human neutrophils; Myeloperoxidase; Free radicals ID HUMAN POLYMORPHONUCLEAR LEUKOCYTES; PEROXIDASE-CATALYZED OXIDATION; MYELOPEROXIDASE COMPOUND-I; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; RAT HEPATOCYTES; ANTIINFLAMMATORY DRUGS; SULFUR-DIOXIDE; NADPH OXIDASE; SO5-RADICALS AB The objective of this study was to determine the effect of (bi)sulfite (hydrated sulfur dioxide) on human neutrophils and the ability of these immune cells to produce reactive free radicals due to (bi)sulfite oxidation. Myeloperoxidase (MPO) is an abundant heme protein in neutrophils that catalyzes the formation of cytotoxic oxidants implicated in asthma and inflammatory disorders. In this study sulfite ((SO3-)-S-center dot) and sulfate (SO4 center dot-) anion radicals are characterized with the ESR spin-trapping technique using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in the reaction of (bi)sulfite oxidation by human MPO and human neutrophils via sulfite radical chain reaction chemistry. After treatment with (bi)sulfite, phorbol 12-myristate 13-acetate-stimulated neutrophils produced DMPO-sulfite anion radical, -superoxide, and -hydroxyl radical adducts. The last adduct probably resulted, in part, from the conversion of DMPO-sulfate to DMPO-hydroxyl radical adduct via a nucleophilic substitution reaction of the radical adduct. This anion radical (SO4 center dot-) is highly reactive and, presumably, can oxidize target proteins to protein radicals, thereby initiating protein oxidation. Therefore, we propose that the potential toxicity of (bi)sulfite during pulmonary inflammation or lung-associated diseases such as asthma may be related to free radical formation. Published by Elsevier Inc. C1 [Ranguelova, Kalina; Triquigneaux, Mathilde; Mason, Ronald P.] NIEHS, Lab Toxicol & Pharmacol, NIH, Res Triangle Pk, NC 27709 USA. [Rice, Annette B.; Garantziotis, Stavros] NIEHS, Clin Res Unit, NIH, Res Triangle Pk, NC 27709 USA. [Khajo, Abdelahad; Magliozzo, Richard S.] CUNY Brooklyn Coll, Dept Chem, Brooklyn, NY 11210 USA. [Khajo, Abdelahad; Magliozzo, Richard S.] CUNY, Grad Ctr, Dept Chem, New York, NY USA. [Khajo, Abdelahad; Magliozzo, Richard S.] CUNY, Grad Ctr, Dept Biochem, New York, NY USA. RP Mason, RP (reprint author), NIEHS, Lab Toxicol & Pharmacol, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. EM mason4@niehs.nih.gov RI Garantziotis, Stavros/A-6903-2009 OI Garantziotis, Stavros/0000-0003-4007-375X FU National Institute of Environmental Health Sciences, National Institutes of Health FX We acknowledge Mary J. Mason, Dr. Ann Motten, and Jean Corbett for their editing of the manuscript. Also, we thank Ms. Brenda Yingling and Ms. Jamie D. Marshburn for their technical assistance. This work has been supported by the Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health. NR 68 TC 28 Z9 29 U1 10 U2 44 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD APR 15 PY 2012 VL 52 IS 8 BP 1264 EP 1271 DI 10.1016/j.freeradbiomed.2012.01.016 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 920WQ UT WOS:000302439900002 PM 22326772 ER PT J AU Horvath, B Mukhopadhyay, P Kechrid, M Patel, V Tanchian, G Wink, DA Gertsch, J Pacher, P AF Horvath, Bela Mukhopadhyay, Partha Kechrid, Malek Patel, Vivek Tanchian, Galin Wink, David A. Gertsch, Juerg Pacher, Pal TI beta-Caryophyllene ameliorates cisplatin-induced nephrotoxicity in a cannabinoid 2 receptor-dependent manner SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE Nephropathy; Cisplatin; (E)-beta-caryophyllene; Cannabinoid 2 receptor; Inflammation; Oxidative stress; Cell death; Free radicals ID HEPATIC ISCHEMIA/REPERFUSION INJURY; SODIUM-INDUCED COLITIS; NITRIC-OXIDE; REACTIVE OXYGEN; ENDOCANNABINOID SYSTEM; OXIDATIVE STRESS; CELL-DEATH; INDUCED CARDIOMYOPATHY; HUMAN CARDIOMYOCYTES; CB2 RECEPTORS AB (E)-beta-caryophyllene (BCP) is a natural sesquiterpene found in many essential oils of spice (best known for contributing to the spiciness of black pepper) and food plants with recognized anti-inflammatory properties. Recently it was shown that BCP is a natural agonist of endogenous cannabinoid 2 (CB2) receptors, which are expressed in immune cells and mediate anti-inflammatory effects. In this study we aimed to test the effects of BCP in a clinically relevant murine model of nephropathy (induced by the widely used antineoplastic drug cisplatin) in which the tubular injury is largely dependent on inflammation and oxidative/nitrative stress. beta-caryophyllene dose-dependently ameliorated cisplatin-induced kidney dysfunction, morphological damage, and renal inflammatory response (chemokines MCP-1 and MIP-2, cytokines TNF-alpha and IL-1 beta, adhesion molecule ICAM-1, and neutrophil and macrophage infiltration). It also markedly mitigated oxidative/nitrative stress (NOX-2 and NOX-4 expression, 4-HNE and 3-NT content) and cell death. The protective effects of BCP against biochemical and histological markers of nephropathy were absent in CB2 knockout mice. Thus. BCP may be an excellent therapeutic agent to prevent cisplatin-induced nephrotoxicity through a CB2 receptor-dependent pathway. Given the excellent safety profile of BCP in humans it has tremendous therapeutic potential in a multitude of diseases associated with inflammation and oxidative stress. Published by Elsevier Inc. C1 [Horvath, Bela; Mukhopadhyay, Partha; Kechrid, Malek; Patel, Vivek; Tanchian, Galin; Pacher, Pal] NIAAA, Lab Physiol Studies, NIH, Bethesda, MD 20892 USA. [Wink, David A.] NCI, Radiat Biol Branch, NIH, Bethesda, MD 20892 USA. [Gertsch, Juerg] Univ Bern, Inst Biochem & Mol Med, CH-3012 Bern, Switzerland. RP Pacher, P (reprint author), NIAAA, Lab Physiol Studies, NIH, Bethesda, MD 20892 USA. EM pacher@mail.nih.gov RI Pacher, Pal/B-6378-2008; MUKHOPADHYAY, PARTHA/G-3890-2010; Horvath, Bela/A-7368-2009 OI Pacher, Pal/0000-0001-7036-8108; MUKHOPADHYAY, PARTHA/0000-0002-1178-1274; FU NIH/NIAAA; Hungarian Scientific Research Fund [OTKA-NKTH-EU MB08-80238]; [HL072889] FX This study was supported by the Intramural Research Program of the NIH/NIAAA (to P.P.) and HL072889 (to B.H.). Dr. Bela Horvath is the recipient of a Hungarian Scientific Research Fund Fellowship (OTKA-NKTH-EU MB08-80238). The authors are indebted to Dr. George Kunos for continuous support. Dr. Pacher dedicates this study to his beloved mother Iren Bolfert, who died from complications of chemotherapy. NR 71 TC 37 Z9 39 U1 0 U2 21 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD APR 15 PY 2012 VL 52 IS 8 BP 1325 EP 1333 DI 10.1016/j.freeradbiomed.2012.01.014 PG 9 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 920WQ UT WOS:000302439900008 PM 22326488 ER PT J AU Lin, BR Natarajan, V AF Lin, Bor-Ruei Natarajan, Ven TI Negative regulation of human U6 snRNA promoter by p38 kinase through Oct-1 SO GENE LA English DT Article DE U6 promoter; Oct-1; p38 kinase; SB202190; MAPK ID ACTIVATED PROTEIN-KINASE; RNA POLYMERASE-III; SMALL NUCLEAR-RNA; TRANSCRIPTION FACTOR OCT-1; DNA-BINDING ACTIVITY; MAP KINASE; POU DOMAIN; CELL-SURVIVAL; OXIDATIVE STRESS; DOWN-REGULATION AB Recruitment of Oct-1 protein to the octamer sequence of U6 promoter is critical for optimal transcription by RNA polymerase III. Here we report that p38 kinase inhibitors, SB202190 and SB203580, stimulated U6 promoter activity and this stimulation can be observed only in the presence of octamer sequence. SB202190-treated cell nuclear extract had about 50% increase in Oct-1 binding activity suggesting that the increased U6 promoter activity by p38 kinase inhibitor is mediated through Oct-1. Mutation in octamer sequence significantly reduced the SB202190-stimulated U6 promoter transcription and the distance between octamer and proximal sequence element of U6 promoter is also critical for the p38 kinase inhibitor-stimulated activity. Exogenous Oct-1 expression showed a concentration-dependent activation of U6 promoter that was further stimulated by the p38 kinase inhibitors. When cells were treated with p38 kinase inducer, hydrogen peroxide or phorbol 12-myristate 13-acetate (PMA), U6 promoter activity was down regulated and this inhibition was reversed by p38 kinase inhibitors. Over-expression of p38 alpha kinase down-regulated U6 promoter activity and this inhibition was further enhanced by PMA and p38 kinase inhibitors reversed this inhibition. p38 kinase inhibitor-treated cells had 50% more U6 RNA than the control cells. Taken together, our results show a negative correlation between the p38 kinase levels and Oct-1 binding on U6 promoter, suggesting that U6 promoter is negatively regulated by p38 kinase. (C) 2012 Elsevier B.V. All rights reserved. C1 [Lin, Bor-Ruei; Natarajan, Ven] NCI, SAIC Frederick, Frederick, MD 21702 USA. RP Lin, BR (reprint author), NCI, SAIC Frederick, 1050 Boyles St, Frederick, MD 21702 USA. EM linbr@mail.nih.gov; vnatarajan@mail.nih.gov FU National Institute of Allergy and Infectious Diseases; National Cancer Institute, National Institutes of Health [HHSN261200800001E] FX This work was supported by the National Institute of Allergy and Infectious Diseases. This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract [HHSN261200800001E]. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. NR 60 TC 6 Z9 7 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD APR 15 PY 2012 VL 497 IS 2 BP 200 EP 207 DI 10.1016/j.gene.2012.01.041 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 922YH UT WOS:000302584300009 PM 22310390 ER PT J AU Yi, L Donsante, A Kennerson, ML Mercer, JFB Garbern, JY Kaler, SG AF Yi, Ling Donsante, Anthony Kennerson, Marina L. Mercer, Julian F. B. Garbern, James Y. Kaler, Stephen G. TI Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy SO HUMAN MOLECULAR GENETICS LA English DT Article ID COPPER-DEFICIENCY MYELONEUROPATHY; INCLUSION-BODY MYOPATHY; OCCIPITAL-HORN-SYNDROME; MENKES-DISEASE; NEURON DISEASE; FRONTOTEMPORAL DEMENTIA; REGULATED TRAFFICKING; HIPPOCAMPAL-NEURONS; MUSCULAR-ATROPHY; PLASMA-MEMBRANE AB ATP7A is a P-type ATPase that regulates cellular copper homeostasis by activity at the trans-Golgi network (TGN) and plasma membrane (PM), with the location normally governed by intracellular copper concentration. Defects in ATP7A lead to Menkes disease or its milder variant, occipital horn syndrome or to a newly discovered condition, ATP7A-related distal motor neuropathy (DMN), for which the precise pathophysiology has been obscure. We investigated two ATP7A motor neuropathy mutations (T994I, P1386S) previously associated with abnormal intracellular trafficking. In the patients fibroblasts, total internal reflection fluorescence microscopy indicated a shift in steady-state equilibrium of ATP7A(T994I) and ATP7A(P1386S), with exaggerated PM localization. Transfection of Hek293T cells and NSC-34 motor neurons with the mutant alleles tagged with the Venus fluorescent protein also revealed excess PM localization. Endocytic retrieval of the mutant alleles from the PM to the TGN was impaired. Immunoprecipitation assays revealed an abnormal interaction between ATP7A(T994I) and p97/VCP, an ubiquitin-selective chaperone which is mutated in two autosomal dominant forms of motor neuron disease: amyotrophic lateral sclerosis and inclusion body myopathy with early-onset Paget disease and fronto-temporal dementia. Small-interfering RNA (SiRNA) knockdown of p97/VCP corrected ATP7A(T994I) mislocalization. Flow cytometry documented that non-permeabilized ATP7A(P1386S) fibroblasts bound a carboxyl-terminal ATP7A antibody, consistent with relocation of the ATP7A di-leucine endocytic retrieval signal to the extracellular surface and partially destabilized insertion of the eighth transmembrane helix. Our findings illuminate the mechanisms underlying ATP7A-related DMN and establish a link between p97/VCP and genetically distinct forms of motor neuron degeneration. C1 [Yi, Ling; Donsante, Anthony; Kaler, Stephen G.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Human Copper Metab, Program Mol Med, Bethesda, MD USA. [Kennerson, Marina L.] Univ Sydney, ANZAC Res Inst, Northcott Neurosci Lab, Concord, Australia. [Mercer, Julian F. B.] Deakin Univ, Sch Life & Environm Sci, Ctr Cellular & Mol Biol, Burwood, Australia. [Garbern, James Y.] Univ Rochester, Sch Med & Dent, Dept Neurol, Rochester, NY 14642 USA. [Garbern, James Y.] Univ Rochester, Sch Med & Dent, Ctr Translat Neuromed, Rochester, NY 14642 USA. RP Kaler, SG (reprint author), NIH, Bldg 10,Room 10N313,10 Ctr Dr,MSC 1853, Bethesda, MD 20892 USA. EM kalers@mail.nih.gov RI Kennerson, Marina/B-5058-2014 FU Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health FX The work was supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health. NR 50 TC 27 Z9 27 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 15 PY 2012 VL 21 IS 8 BP 1794 EP 1807 DI 10.1093/hmg/ddr612 PG 14 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 919DS UT WOS:000302302400010 PM 22210628 ER PT J AU Liu, CQ Bakeri, H Li, TS Swaroop, A AF Liu, Chunqiao Bakeri, Hirva Li, Tiansen Swaroop, Anand TI Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma SO HUMAN MOLECULAR GENETICS LA English DT Article ID INTERKINETIC NUCLEAR MIGRATION; GANGLION-CELL; SONIC HEDGEHOG; VERTEBRATE RETINA; MOUSE RETINA; TRANSCRIPTION FACTORS; FATE DETERMINATION; NEURAL DEVELOPMENT; MAMMALIAN RETINA; NERVOUS-SYSTEM AB Nineteen Wnt ligands and 10 Frizzled (Fz) receptors mediate multiple distinct cellular events during neuronal development. However, their precise roles in cell-type specification and organogenesis are poorly delineated because of overlapping functions and expression profiles. Here, we have explored the role of two closely related Frizzled receptors, Fz5 and Fz8, in mouse retinal development. We previously showed that Fz5(/) mice exhibit mild coloboma and microphthalmia at approximate to 50 penetrance. Fz8 expression overlaps with Fz5 in the neural retina and optic fissure/disc. Mice lacking Fz8 show minimal eye and retinal defects. The embryos lacking both Fz5 and Fz8 die early in development, but a majority of triallelic Fz5(/);Fz8(/) mutants survive until birth. The triallelic mutant develops severe retinal coloboma and microphthalmia with full penetrance. At the cellular level, impaired neurogenesis is indicated by increased early-born retinal neurons that result from accelerated cell cycle exit of progenitors. Deficiency of apical retinal neuroepithelium is indicated by altered localization of apical junction markers, such as atypical protein kinase C, RhoA and -catenin. Hes1 expression, which is critical for retinal progenitor expansion, is down-regulated in the triallelic mutant mouse. Furthermore, blocking Frizzled receptors in cultured retinal explants led to basally shifted divisions of retinal progenitors. Together, our studies suggest a dose-dependent regulation of signaling by Fz5 and Fz8 in optic fissure/disc formation and progenitor expansion. C1 [Liu, Chunqiao; Bakeri, Hirva; Li, Tiansen; Swaroop, Anand] NEI, N NRL, Bethesda, MD 20892 USA. RP Liu, CQ (reprint author), NEI, N NRL, MSC0610,6 Ctr Dr, Bethesda, MD 20892 USA. EM cqliu@nei.nih.gov; swaroopa@nei.nih.gov RI Liu, Chunqiao/O-3391-2013; OI Liu, Chunqiao/0000-0002-0299-7401; Swaroop, Anand/0000-0002-1975-1141 FU National Eye Institute FX This research was supported by intramural program of the National Eye Institute. NR 59 TC 16 Z9 16 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 15 PY 2012 VL 21 IS 8 BP 1848 EP 1860 DI 10.1093/hmg/ddr616 PG 13 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 919DS UT WOS:000302302400014 PM 22228100 ER PT J AU Tang, W Fu, YP Figueroa, JD Malats, N Garcia-Closas, M Chatterjee, N Kogevinas, M Baris, D Thun, M Hall, JL De Vivo, I Albanes, D Porter-Gill, P Purdue, MP Burdett, L Liu, LY Hutchinson, A Myers, T Tardon, A Serra, C Carrato, A Garcia-Closas, R Lloreta, J Johnson, A Schwenn, M Karagas, MR Schned, A Black, A Jacobs, EJ Diver, WR Gapstur, SM Virtamo, J Hunter, DJ Fraumeni, JF Chanock, SJ Silverman, DT Rothman, N Prokunina-Olsson, L AF Tang, Wei Fu, Yi-Ping Figueroa, Jonine D. Malats, Nuria Garcia-Closas, Montserrat Chatterjee, Nilanjan Kogevinas, Manolis Baris, Dalsu Thun, Michael Hall, Jennifer L. De Vivo, Immaculata Albanes, Demetrius Porter-Gill, Patricia Purdue, Mark P. Burdett, Laurie Liu, Luyang Hutchinson, Amy Myers, Timothy Tardon, Adonina Serra, Consol Carrato, Alfredo Garcia-Closas, Reina Lloreta, Josep Johnson, Alison Schwenn, Molly Karagas, Margaret R. Schned, Alan Black, Amanda Jacobs, Eric J. Diver, W. Ryan Gapstur, Susan M. Virtamo, Jarmo Hunter, David J. Fraumeni, Joseph F., Jr. Chanock, Stephen J. Silverman, Debra T. Rothman, Nathaniel Prokunina-Olsson, Ludmila TI Mapping of the UGT1A locus identifies an uncommon coding variant that affects mRNA expression and protects from bladder cancer SO HUMAN MOLECULAR GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; HUMAN UDP-GLUCURONOSYLTRANSFERASES; CLINICAL-APPLICATION; COMPLEX DISEASES; BILIRUBIN LEVELS; RARE VARIANTS; GENE-COMPLEX; URINARY PH; NAT2 GENES; POLYMORPHISMS AB A recent genome-wide association study of bladder cancer identified the UGT1A gene cluster on chromosome 2q37.1 as a novel susceptibility locus. The UGT1A cluster encodes a family of UDP-glucuronosyltransferases (UGTs), which facilitate cellular detoxification and removal of aromatic amines. Bioactivated forms of aromatic amines found in tobacco smoke and industrial chemicals are the main risk factors for bladder cancer. The association within the UGT1A locus was detected by a single nucleotide polymorphism (SNP) rs11892031. Now, we performed detailed resequencing, imputation and genotyping in this region. We clarified the original genetic association detected by rs11892031 and identified an uncommon SNP rs17863783 that explained and strengthened the association in this region (allele frequency 0.014 in 4035 cases and 0.025 in 5284 controls, OR 0.55, 95CI 0.440.69, P 3.3 10(7)). Rs17863783 is a synonymous coding variant Val209Val within the functional UGT1A6.1 splicing form, strongly expressed in the liver, kidney and bladder. We found the protective T allele of rs17863783 to be associated with increased mRNA expression of UGT1A6.1 in in-vitro exontrap assays and in human liver tissue samples. We suggest that rs17863783 may protect from bladder cancer by increasing the removal of carcinogens from bladder epithelium by the UGT1A6.1 protein. Our study shows an example of genetic and functional role of an uncommon protective genetic variant in a complex human disease, such as bladder cancer. C1 [Prokunina-Olsson, Ludmila] NCI, Lab Translat Genom, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Malats, Nuria] Spanish Natl Canc Res Ctr, Madrid 28029, Spain. [Garcia-Closas, Montserrat] Inst Canc Res, Div Genet & Epidemiol, London SW7 3RP, England. [Kogevinas, Manolis] Ctr Res Environm Epidemiol CREAL, Barcelona 08003, Spain. [Kogevinas, Manolis] Municipal Inst Med Res, Barcelona 08003, Spain. [Kogevinas, Manolis; Tardon, Adonina] CIBERESP, Barcelona 08003, Spain. [Kogevinas, Manolis] Natl Sch Publ Hlth, Athens 11521, Greece. [Thun, Michael; Jacobs, Eric J.; Diver, W. Ryan; Gapstur, Susan M.] Amer Canc Soc, Epidemiol Res Program, Atlanta, GA 30303 USA. [Hall, Jennifer L.] Univ Minnesota, Lillehei Heart Inst, Dept Med, Minneapolis, MN 55455 USA. [De Vivo, Immaculata] Brigham & Womens Hosp, Channing Lab, Dept Med, Boston, MA 02115 USA. [Burdett, Laurie; Hutchinson, Amy; Myers, Timothy] NCI, Core Genotype Facil, SAIC Frederick Inc, Frederick, MD 21702 USA. [Tardon, Adonina] Univ Oviedo, Oviedo 33003, Spain. [Serra, Consol] Univ Pompeu Fabra, Barcelona 08002, Spain. [Carrato, Alfredo] Ramon y Cajal Univ Hosp, Madrid 28034, Spain. [Garcia-Closas, Reina] Hosp Univ Canarias, Unidad Invest, San Cristobal la Laguna 38320, Spain. [Lloreta, Josep] Univ Pompeu Fabra, Hosp Mar, IMIM, Barcelona 08003, Spain. [Johnson, Alison] Vermont Canc Registry, Burlington, VT 05401 USA. [Schwenn, Molly] Maine Canc Registry, Augusta, ME 04333 USA. [Karagas, Margaret R.] Dartmouth Med Sch, Hanover, NH 03755 USA. [Schned, Alan] Washington Univ, Sch Med, Dept Urol, St Louis, MO 63110 USA. [Virtamo, Jarmo] Natl Inst Hlth & Welf, Helsinki 00271, Finland. [Hunter, David J.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Program Mol & Genet Epidemiol, Boston, MA 02115 USA. RP Prokunina-Olsson, L (reprint author), NCI, Lab Translat Genom, Div Canc Epidemiol & Genet, NIH, 8717 Grovemont Circle, Bethesda, MD 20892 USA. EM prokuninal@mail.nih.gov RI Tang, Wei/H-7103-2013; Serra, C/E-6879-2014; Lloreta, J/I-2112-2014; Albanes, Demetrius/B-9749-2015; Garcia-Closas, Montserrat /F-3871-2015; Malats, Nuria/H-7041-2015; Purdue, Mark/C-9228-2016; Kogevinas, Manolis/C-3918-2017 OI Tang, Wei/0000-0002-7089-4391; Prokunina-Olsson, Ludmila/0000-0002-9622-2091; Serra, C/0000-0001-8337-8356; Lloreta, J/0000-0003-1644-9470; Garcia-Closas, Montserrat /0000-0003-1033-2650; Malats, Nuria/0000-0003-2538-3784; Purdue, Mark/0000-0003-1177-3108; FU National Institutes of Health, National Cancer Institute [HHSN261200800001E]; Centro Nacional de Investigaciones Oncologicas, Madrid, Spain; DCEG, NCI/NIH, Rockville, MD, USA; Information Management Services, Silver Spring, MD, USA; Institut Municipal d'Investigacio Medica, Barcelona, Spain; Westat, Inc., Rockville, MD, USA; Marques de Valdecilla University Hospital, Santander, Cantabria, Spain; Hospital Ciudad de Coria, Coria (Caceres), Spain; Westat, Rockville, MD, USA; Information Management Services, Inc., Silver Spring, MD, USA; SBCS of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics and intramural [NCI N02-CP-11015]; FIS/Spain [98/1274, 00/0745, PI061614, G03/174]; Fundacio Marato TV3; Red Tematica Investigacion Cooperativa en Cancer (RTICC); Consolider ONCOBIO; NEBCS of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics and intramural [NCI N02-CP-01037]; PLCO -The NIH Genes, Environment and Health Initiative (GEI); DNA extraction and statistical analyses [HG-06-033-NCI-01, RO1HL091172-01]; Johns Hopkins University Center for Inherited Disease Research [U01HG004438, HHSN268200782096C]; GENEVA -The NIH Genes, Environment and Health Initiative [GEI] [HG-06-033-NCI-01, RO1HL091172-01]; GENEVA Coordination Center [U01 HG004446]; National Institutes of Health, NCI, Division of Cancer Epidemiology and Genetics; Division of Cancer Epidemiology and Genetics; Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, ATBC; NIH; National Cancer Institute; US Public Health Service from the National Cancer Institute, Department of Health and Human Services [N01-CN-45165, N01-RC-45035, N01-RC-37004]; NHS; HPFS [CA055075, CA087969]; [EU-FP7-201663]; [RO1-CA089715]; [CA34627] FX The NCI bladder cancer GWAS and follow-up studies are supported by the intramural research program of the National Institutes of Health, National Cancer Institute.; Following individuals are acknowledged for their support: Francisco Real (Molecular Pathology Programme, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain). Marie-Joseph Horner (DCEG, NCI/NIH, Rockville, MD, USA). Adam Mumy (DCEG, NCI/NIH, Rockville, MD, USA). Natalia Orduz (DCEG, NCI/NIH, Rockville, MD, USA). Leslie Carroll (Information Management Services, Silver Spring, MD, USA). Gemma Castano-Vinyals (Institut Municipal d'Investigacio Medica, Barcelona, Spain). Fernando Fernandez (Institut Municipal d'Investigacio Medica, Barcelona, Spain). Paul Hurwitz (Westat, Inc., Rockville, MD, USA). Charles Lawrence (Westat, Inc., Rockville, MD, USA). Marta Lopez-Brea (Marques de Valdecilla University Hospital, Santander, Cantabria, Spain). Anna McIntosh (Westat, Inc., Rockville, MD, USA). Angeles Panadero (Hospital Ciudad de Coria, Coria (Caceres), Spain). Fernando Rivera (Marques de Valdecilla University Hospital, Santander, Cantabria, Spain). Robert Saal (Westat, Rockville, MD, USA). Maria Sala (Institut Municipal d'Investigacio Medica, Barcelona, Spain). Kirk Snyder (Information Management Services, Inc., Silver Spring, MD, USA). Anne Taylor (Information Management Services, Inc., Silver Spring, MD, USA). Montserrat Tora (Institut Municipal d'Investigacio Medica, Barcelona, Spain). Jane Wang (Information Management Services, Silver Spring, MD, USA).; This project has been funded in part with federal funds from the National Cancer, Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.; Support for individual studies that participated in the effort is as follows: SBCS (D.T.S.)-Intramural Research Program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics and intramural, contract number NCI N02-CP-11015. FIS/Spain 98/1274, FIS/Spain 00/0745, PI061614 and G03/174, Fundacio Marato TV3, Red Tematica Investigacion Cooperativa en Cancer (RTICC), Consolider ONCOBIO, EU-FP7-201663; and RO1-CA089715 and CA34627. NEBCS (D.T.S.)-Intramural research program of the National Institutes of Health, National Cancer Institute, Division of Cancer Epidemiology and Genetics and intramural, contract number NCI N02-CP-01037, PLCO (M.P.P.)-The NIH Genes, Environment and Health Initiative (GEI) partly funded, DNA extraction and statistical analyses (HG-06-033-NCI-01 and RO1HL091172-01), genotyping at the Johns Hopkins University Center for Inherited Disease Research (U01HG004438 and NIH HHSN268200782096C) and study coordination at the GENEVA (N.C.)-The NIH Genes, Environment and Health Initiative [GEI] partly funded DNA extraction and statistical analyses (HG-06-033-NCI-01 and RO1HL091172-01), genotyping at the Johns Hopkins University Center for Inherited Disease Research (U01HG004438 and NIH HHSN268200782096C) and study coordination at the GENEVA Coordination Center (U01 HG004446) for EAGLE and part of PLCO studies. Genotyping for the remaining part of PLCO and all ATBC and CPS-II samples were supported by the Intramural Research Program of the National Institutes of Health, NCI, Division of Cancer Epidemiology and Genetics. The PLCO is supported by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics and supported by contracts from the Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, ATBC (D.A.)-This research was supported in part by the Intramural Research Program of the NIH and the National Cancer Institute. Additionally, this research was supported by US Public Health Service contracts N01-CN-45165, N01-RC-45035 and N01-RC-37004 from the National Cancer Institute, Department of Health and Human Services. NHS & HPFS (I.D.V.)-CA055075 and CA087969. NR 64 TC 36 Z9 36 U1 2 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD APR 15 PY 2012 VL 21 IS 8 BP 1918 EP 1930 DI 10.1093/hmg/ddr619 PG 13 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA 919DS UT WOS:000302302400020 PM 22228101 ER PT J AU Dooley, KE Park, JG Swindells, S Allen, R Haas, DW Cramer, Y Aweeka, F Wiggins, I Gupta, A Lizak, P Qasba, S van Heeswijk, R Flexner, C AF Dooley, Kelly E. Park, Jeong-Gun Swindells, Susan Allen, Reena Haas, David W. Cramer, Yoninah Aweeka, Francesca Wiggins, Ilene Gupta, Amita Lizak, Patricia Qasba, Sonia van Heeswijk, Rolf Flexner, Charles CA ACTG 5267 Study Team TI Safety, Tolerability, and Pharmacokinetic Interactions of the Antituberculous Agent TMC207 (Bedaquiline) With Efavirenz in Healthy Volunteers: AIDS Clinical Trials Group Study A5267 SO JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Article DE bedaquiline; efavirenz; CYP3A; HIV; pharmacokinetics; TMC207; tuberculosis ID MULTIDRUG-RESISTANT TUBERCULOSIS; DRUG-INDUCED PHOSPHOLIPIDOSIS; ANTIRETROVIRAL THERAPY; MYCOBACTERIUM-TUBERCULOSIS; DIARYLQUINOLINE TMC207; ATP SYNTHASE; R207910; CYP2B6; MODEL; PYRAZINAMIDE AB Background: Drug-drug interactions complicate management of coinfection with HIV-1 and Mycobacterium tuberculosis. Bedaquiline (formerly TMC207), an investigational agent for the treatment of tuberculosis, is metabolized by cytochrome P450 (CYP) 3A which may be induced by the antiretroviral drug efavirenz. Methods: This was a phase 1 pharmacokinetic drug interaction trial. Each healthy volunteer received two 400 mg doses of bedaquiline, the first alone and the second with concomitant steady-state efavirenz. Plasma pharmacokinetic sampling for bedaquiline and its N-monodesmethyl metabolite was performed over 14 days after each bedaquiline dose. Steady-state efavirenz pharmacokinetics were also determined. Efavirenz metabolizer status was based on CYP2B6 composite 516/983 genotype. Results: Thirty-three of 37 enrolled subjects completed the study. Geometric mean of ratios for bedaquiline with efavirenz versus bedaquiline alone were 0.82 [90% confidence interval (CI): 0.75 to 0.89] for the 14-day area under the concentration-time curve (AUC(0-336) (h)) and 1.00 (90% CI: 0.88 to 1.13) for the maximum concentration (C-max). For N-monodesmethyl metabolite, the geometric mean of ratios was 1.07 (90% CI: 0.97 to 1.19) for AUC(0-336 h) and 1.89 (90% CI: 1.66 to 2.15) for C-max. There were no grade 3 or 4 clinical adverse events. One subject developed asymptomatic grade 3 serum transaminase elevation, prompting study drug discontinuation. Efavirenz concentrations stratified by CYP2B6 genotype were similar to historical data. Conclusions: Single-dose bedaquiline was well tolerated alone and with steady-state efavirenz. The effect of efavirenz on bedaquiline concentrations is unlikely to be clinically significant. C1 [Dooley, Kelly E.; Wiggins, Ilene; Gupta, Amita; Flexner, Charles] Johns Hopkins Univ, Sch Med, Baltimore, MD 21287 USA. [Park, Jeong-Gun; Cramer, Yoninah] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. [Swindells, Susan] Univ Nebraska Med Ctr, Omaha, NE USA. [Allen, Reena] AIDS Clin Trials Grp Operat, Silver Spring, MD USA. NIH, Div Aids, Bethesda, MD 20892 USA. [Haas, David W.] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. [Aweeka, Francesca; Lizak, Patricia] Univ Calif San Francisco, San Francisco, CA 94143 USA. [van Heeswijk, Rolf] Tibotec, BVBA, Mechelen, Belgium. [Qasba, Sonia] Montgomery Cty Dept Hlth & Human Serv, Silver Spring, MD USA. RP Dooley, KE (reprint author), Johns Hopkins Univ, Sch Med, 600 N Wolfe St,Osler 527, Baltimore, MD 21287 USA. EM kdooley1@jhmi.edu FU AIDS Clinical Trials Group; National Institute of Allergy and Infectious Diseases; NCRR/NI [UL1 RR024975, U01-AI06439]; ACTG [5-U01 AI069423]; CTSA [UL 1RR 025747 CTSA]; National Center of Research Resources of the NIH [U01 AI069474, UL1-RR025755]; RPh-Johns Hopkins Adult AIDS CRS Site 201 CTU [AI69465]; GCRC [U54-RR023561]; [U01 AI068636]; [AI068634]; [U01 AI069497]; [U01 AI069465]; [AI0699450]; [U01 AI069439]; [R01 AI077505]; [K23AI080842] FX Supported by the AIDS Clinical Trials Group sponsored by the National Institute of Allergy and Infectious Diseases and was supported by the following grants: U01 AI068636 and AI068634, U01 AI069497 (A.G.), U01 AI069465 (A.G., C.F., I.W.) AI0699450 (S.S.), U01 AI069439, R01 AI077505 (D.W.H.), and K23AI080842 (K.E.D.). The AIDS Clinical Trials Group Human DNA Repository is supported in part by grant UL1 RR024975. Tibotec Pharmaceuticals and Bristol-Myers Squibb provided study medications.; Deborah Sutherland, RN, BSN, CCRP and Marcia Free, RN, BSN, CCRP-Vanderbilt Therapeutics Clinical Research Site #3652 NIH/ACTG Grant U01-AI06439, Vanderbilt CTSA grant UL1 RR024975 from NCRR/NI#.; Miriam Chicurel, BS, BSN and Charles W. Tedder, BA- UNC AIDS Clinical Trials Unit Site 3201, ACTG Grant 5-U01 AI069423, CTSA Grant UL 1RR 025747 CTSA.; Susan L. Koletar, MD and Heather Harber, RN-The Ohio State University, 2301 ACTG Grant U01 AI069474, Clinical Research Center, Grant UL1-RR025755 from the National Center of Research Resources of the NIH.; Annie Luetkemeyer, MD and Jay Dwyer, RN- UCSF AIDS CRS Site 801 Grant # 5UO1 AI069502. Judith Hackman, RN and Andrea Weiss, RPh-Johns Hopkins Adult AIDS CRS Site 201 CTU Grant AI69465; GCRC Grant U54-RR023561. NR 36 TC 27 Z9 29 U1 1 U2 16 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 J9 JAIDS-J ACQ IMM DEF JI JAIDS PD APR 15 PY 2012 VL 59 IS 5 BP 455 EP 462 DI 10.1097/QAI.0b013e3182410503 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 917BQ UT WOS:000302147400010 PM 22126739 ER PT J AU Kundu, P Inati, SJ Evans, JW Luh, WM Bandettini, PA AF Kundu, Prantik Inati, Souheil J. Evans, Jennifer W. Luh, Wen-Ming Bandettini, Peter A. TI Differentiating BOLD and non-BOLD signals in fMRI time series using multi-echo EPI SO NEUROIMAGE LA English DT Article DE fMRI; Resting state; Multi-echo; BOLD; TE dependence; ICA; Denoising; Subcortical; Connectivity ID INDEPENDENT COMPONENT ANALYSIS; HUMAN BRAIN-FUNCTION; FUNCTIONAL CONNECTIVITY; CONTRAST SENSITIVITY; SENSORY STIMULATION; 1.5 T; MRI; ACTIVATION; NOISE; OXYGENATION AB A central challenge in the fMRI based study of functional connectivity is distinguishing neuronally related signal fluctuations from the effects of motion, physiology, and other nuisance sources. Conventional techniques for removing nuisance effects include modeling of noise time courses based on external measurements followed by temporal filtering. These techniques have limited effectiveness. Previous studies have shown using multi-echo fMRI that neuronally related fluctuations are Blood Oxygen Level Dependent (BOLD) signals that can be characterized in terms of changes in R-2* and initial signal intensity (S-0) based on the analysis of echo-time (TE) dependence. We hypothesized that if TE-dependence could be used to differentiate BOLD and non-BOLD signals, non-BOLD signal could be removed to denoise data without conventional noise modeling. To test this hypothesis, whole brain multi-echo data were acquired at 3 TEs and decomposed with Independent Components Analysis (ICA) after spatially concatenating data across space and TE. Components were analyzed for the degree to which their signal changes fit models for R-2* and S-0 change, and summary scores were developed to characterize each component as BOLD-like or not BOLD-like. These scores clearly differentiated BOLD-like "functional network" components from non BOLD-like components related to motion, pulsatility, and other nuisance effects. Using non BOLD-like component time courses as noise regressors dramatically improved seed-based correlation mapping by reducing the effects of high and low frequency non-BOLD fluctuations. A comparison with seed-based correlation mapping using conventional noise regressors demonstrated the superiority of the proposed technique for both individual and group level seed-based connectivity analysis, especially in mapping subcortical-cortical connectivity. The differentiation of BOLD and non-BOLD components based on TE-dependence was highly robust, which allowed for the identification of BOLD-like components and the removal of non BOLD-like components to be implemented as a fully automated procedure. Published by Elsevier Inc. C1 [Kundu, Prantik; Evans, Jennifer W.; Bandettini, Peter A.] NIMH, Sect Funct Imaging Methods, Lab Brain & Cognit, NIH, Bethesda, MD 20892 USA. [Inati, Souheil J.; Luh, Wen-Ming; Bandettini, Peter A.] NIMH, Funct MRI Facil, NIH, Bethesda, MD 20892 USA. [Kundu, Prantik] Univ Cambridge, Addenbrookes Hosp, Dept Psychiat, Cambridge CB2 2QQ, England. [Evans, Jennifer W.] Henry M Jackson Fdn, Ctr Neurosci & Regenerat Med, Rockville, MD 20852 USA. RP Kundu, P (reprint author), NIMH, Sect Funct Imaging Methods, Lab Brain & Cognit, NIH, Bethesda, MD 20892 USA. EM kundup@mail.nih.gov OI Inati, Souheil/0000-0001-5596-9554; Kundu, Prantik/0000-0001-9367-3068 FU National Institute of Mental Health; NIH-Cambridge FX This work was funded by the National Institute of Mental Health Intramural Research Program. Prantik Kundu is supported by the NIH-Cambridge Scholars program. NR 46 TC 77 Z9 77 U1 2 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 15 PY 2012 VL 60 IS 3 BP 1759 EP 1770 DI 10.1016/j.neuroimage.2011.12.028 PG 12 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 917TZ UT WOS:000302202800017 PM 22209809 ER PT J AU Misaki, M Wallace, GL Dankner, N Martin, A Bandettini, PA AF Misaki, Masaya Wallace, Gregory L. Dankner, Nathan Martin, Alex Bandettini, Peter A. TI Characteristic cortical thickness patterns in adolescents with autism spectrum disorders: Interactions with age and intellectual ability revealed by canonical correlation analysis SO NEUROIMAGE LA English DT Article DE Autism spectrum disorders; Kernel canonical correlation analysis; Varimax rotation; Cortical thickness; Developmental change ID SURFACE-BASED ANALYSIS; HUMAN CEREBRAL-CORTEX; DIAGNOSTIC INTERVIEW; BRAIN-DEVELOPMENT; MRI; VOLUME; CHILDREN; SYSTEM; IQ AB To investigate patterns and correlates of cortical thickness in adolescent males with autism spectrum disorders (ASD) versus matched typically developing controls, we applied kernel canonical correlation analysis to whole brain cortical thickness with the explaining variables of diagnosis, age, full-scale IQ and their interactions. The analysis found that canonical variates (patterns of cortical thickness) correlated with each of these variables. The diagnosis- and age-by-diagnosis-related canonical variates showed thinner cortex for participants with ASD, which is consistent with previous studies using a univariate analysis. In addition, the multivariate statistics found larger affected regions with higher sensitivity than those found using univariate analysis. An IQ-related effect was also found with the multivariate analysis. The effects of IQ and age-by-IQ interaction on cortical thickness differed between the diagnostic groups. For typically developing adolescents, IQ was positively correlated with cortical thickness in orbitofrontal, postcentral and superior temporal regions, and greater thinning with age was seen in dorsal frontal areas in the superior IQ (> 120) group. These associations between IQ and cortical thickness were not seen in the ASD group. Differing relationships between IQ and cortical thickness implies independent associations between measures of intelligence and brain structure in ASD versus typically developing controls. We discuss these findings vis-a-vis prior results obtained utilizing univariate methods. Published by Elsevier Inc. C1 [Misaki, Masaya; Wallace, Gregory L.; Dankner, Nathan; Martin, Alex; Bandettini, Peter A.] NIMH, Lab Brain & Cognit, NIH, Bethesda, MD USA. RP Misaki, M (reprint author), Laureate Inst Brain Res, 6655 S Yale Ave, Tulsa, OK 74136 USA. EM mamisaki@gmail.com RI martin, alex/B-6176-2009; OI Wallace, Gregory/0000-0003-0329-5054 FU National Institutes of Health, National Institute of Mental Health (NIMH) FX This study was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Mental Health (NIMH). NR 40 TC 17 Z9 17 U1 1 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR 15 PY 2012 VL 60 IS 3 BP 1890 EP 1901 DI 10.1016/j.neuroimage.2012.01.120 PG 12 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 917TZ UT WOS:000302202800029 PM 22326986 ER PT J AU Wehr, NB Levine, RL AF Wehr, Nancy B. Levine, Rodney L. TI Quantitation of protein carbonylation by dot blot SO ANALYTICAL BIOCHEMISTRY LA English DT Article DE Protein carbonylation; 2,4-Dinitrophenylhydrazine; Oxidative stress; Immunoblot; Dot blot ID OXIDATIVELY MODIFIED PROTEINS; GLUTAMINE-SYNTHETASE; RESIDUES AB Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is frequently measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent, 2,4-dinitrophenylhydrazine. We developed an immunochemical dot blot method for quantitation of protein carbonylation in homogenates or purified proteins. Dimethyl sulfoxide was employed as the solvent because it very efficiently extracts proteins from tissues and keeps them soluble. It also readily dissolves 2,4-dinitrophenylhydrazine and wets polyvinylidene difluoride (PVDF) membranes. The detection limit is 0.19 +/- 0.04 pmol of carbonyl, and 60 ng of protein is sufficient to measure protein carbonyl content. This level of sensitivity allowed measurement of protein carbonylation in individual Drosophila. (C) 2012 Elsevier Inc. All rights reserved. C1 [Wehr, Nancy B.; Levine, Rodney L.] NHLBI, Biochem Lab, Bethesda, MD 20892 USA. RP Levine, RL (reprint author), NHLBI, Biochem Lab, Bldg 3, Bethesda, MD 20892 USA. EM rlevine@nih.gov RI Levine, Rodney/D-9885-2011 FU National Heart, Lung, and Blood Institute FX This research was supported by the Intramural Research Program of the National Heart, Lung, and Blood Institute. We thank Barbara Berlett and Elena Gaidamakova for performing the gamma irradiations. NR 19 TC 10 Z9 10 U1 0 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD APR 15 PY 2012 VL 423 IS 2 BP 241 EP 245 DI 10.1016/j.ab.2012.01.031 PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 919NY UT WOS:000302336100009 PM 22326366 ER PT J AU Zhang, B Noble, PL Winslow, JT Pine, DS Nelson, EE AF Zhang, Bo Noble, Pamela L. Winslow, James T. Pine, Daniel S. Nelson, Eric E. TI Amygdala volume predicts patterns of eye fixation in rhesus monkeys SO BEHAVIOURAL BRAIN RESEARCH LA English DT Article DE Face recognition; MRI; Social; Affiliative; Gaze ID FACIAL-EXPRESSION; NEURAL RESPONSES; SOCIAL ANXIETY; MACACA-MULATTA; AUTISM; GAZE; FACE; ADOLESCENTS; BEHAVIOR; PRIMATE AB In both human and nonhuman primates the eyes are a highly salient feature of the face, conveying identity, emotion and attentional direction of conspecifics. Studies have indicated that the amygdala plays an important role in eye contact, and amygdala dysfunction may underlie social deficits in disorders such as autism through effects on eye contact. In the present study we compared the volume of the amygdala in 32 juvenile rhesus monkeys to visual fixation patterns in a social memory paradigm. Amygdala volume was determined from manual traces of structural MRIs and fixation patterns were assessed using eyetracking methodology. A significant positive relationship was found between amygdala volume and fixation on both the face and the eye region. Amygdala volume was also found to relate to habituation across multiple presentations of different photographs of the same individual monkeys indicating a role in social memory. These data provide an important linkage between structural variation of amygdala and previous demonstrations of function in a nonhuman primate model. Published by Elsevier B.V. C1 [Zhang, Bo; Pine, Daniel S.; Nelson, Eric E.] NIMH, Emot & Affect Neurosci Branch, Bethesda, MD 20892 USA. [Noble, Pamela L.; Winslow, James T.] NIMH, Nonhuman Primate Core Facil, Bethesda, MD 20892 USA. RP Nelson, EE (reprint author), NIMH, Emot & Affect Neurosci Branch, Bldg 15K, Bethesda, MD 20892 USA. EM nelson@mail.nih.gov RI Nelson, Eric/B-8980-2008 OI Nelson, Eric/0000-0002-3376-2453 FU National Institutes of Health, NIMH FX The work presented here was supported entirely by the intramural research program of the National Institutes of Health, NIMH. The authors wish to acknowledge the technical assistance and training in amygdala tracing provided by Julia Scott at the University of California - Davis. We also are indebted to the veterinary and animal care staff of the NIH animal facility in Poolesville and all of the staff at the NIMH primate core. NR 27 TC 2 Z9 2 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-4328 J9 BEHAV BRAIN RES JI Behav. Brain Res. PD APR 15 PY 2012 VL 229 IS 2 BP 433 EP 437 DI 10.1016/j.bbr.2012.01.009 PG 5 WC Behavioral Sciences; Neurosciences SC Behavioral Sciences; Neurosciences & Neurology GA 915TM UT WOS:000302050300018 PM 22285417 ER PT J AU Gwamaka, M Kurtis, JD Sorensen, BE Holte, S Morrison, R Mutabingwa, TK Fried, M Duffy, PE AF Gwamaka, Moses Kurtis, Jonathan D. Sorensen, Bess E. Holte, Sarah Morrison, Robert Mutabingwa, Theonest K. Fried, Michal Duffy, Patrick E. TI Iron Deficiency Protects Against Severe Plasmodium falciparum Malaria and Death in Young Children SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID SERUM TRANSFERRIN RECEPTOR; PLACEBO-CONTROLLED TRIAL; CHELATION-THERAPY; CEREBRAL MALARIA; GAMBIAN CHILDREN; PREGNANT-WOMEN; ORAL IRON; ANEMIA; SUPPLEMENTATION; MORTALITY AB Background. Iron supplementation may increase malaria morbidity and mortality, but the effect of naturally occurring variation in iron status on malaria risk is not well studied. Methods. A total of 785 Tanzanian children living in an area of intense malaria transmission were enrolled at birth, and intensively monitored for parasitemia and illness including malaria for up to 3 years, with an average of 47 blood smears. We assayed plasma samples collected at routine healthy-child visits, and evaluated the impact of iron deficiency (ID) on future malaria outcomes and mortality. Results. ID at routine, well-child visits significantly decreased the odds of subsequent parasitemia (23% decrease, P < .001) and subsequent severe malaria (38% decrease, P = .04). ID was also associated with 60% lower all-cause mortality (P = .04) and 66% lower malaria-associated mortality (P = .11). When sick visits as well as routine healthy-child visits are included in analyses (average of 3 iron status assays/child), ID reduced the prevalence of parasitemia (6.6-fold), hyperparasitemia (24.0-fold), and severe malaria (4.0-fold) at the time of sample collection (all P < .001). Conclusions. Malaria risk is influenced by physiologic iron status, and therefore iron supplementation may have adverse effects even among children with ID. Future interventional studies should assess whether treatment for ID coupled with effective malaria control can mitigate the risks of iron supplementation for children in areas of malaria transmission. C1 [Fried, Michal; Duffy, Patrick E.] NIAID, Lab Malaria Immunol & Vaccinol, NIH, Rockville, MD 20852 USA. [Gwamaka, Moses; Sorensen, Bess E.; Morrison, Robert; Mutabingwa, Theonest K.; Fried, Michal; Duffy, Patrick E.] Seattle Biomed Res Inst, Mother Offspring Malaria Studies Project, Washington, DC USA. [Gwamaka, Moses; Sorensen, Bess E.; Morrison, Robert; Mutabingwa, Theonest K.; Fried, Michal; Duffy, Patrick E.] Muheza Designated Dist Hosp, Muheza, Tanzania. [Gwamaka, Moses] Sokoine Univ Agr, Morogoro, Tanzania. [Kurtis, Jonathan D.] Rhode Isl Hosp, Ctr Int Hlth Res, Providence, RI USA. [Kurtis, Jonathan D.] Brown Univ, Sch Med, Dept Pathol & Lab Med, Providence, RI 02912 USA. [Holte, Sarah] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Mutabingwa, Theonest K.] Natl Inst Med Res, Dar Es Salaam, Tanzania. [Fried, Michal] Univ Washington, Seattle, WA 98195 USA. RP Duffy, PE (reprint author), NIAID, Lab Malaria Immunol & Vaccinol, NIH, 5640 Fishers Ln,Twinbrook Bldg,Rm 1111, Rockville, MD 20852 USA. EM patrick.duffy@nih.gov FU US National Institutes of Health [AI52059]; Bill & Melinda Gates Foundation [1364]; Seattle BioMed FX This work was supported by grants from the US National Institutes of Health (grant AI52059) and the Bill & Melinda Gates Foundation (grant 1364; to P. E. D.). S. H. received grant support from Seattle BioMed. NR 43 TC 53 Z9 55 U1 0 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD APR 15 PY 2012 VL 54 IS 8 BP 1137 EP 1144 DI 10.1093/cid/cis010 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 915EW UT WOS:000302007900019 PM 22354919 ER PT J AU La Rosa, C Longmate, J Lacey, SF Kaltcheva, T Sharan, R Marsano, D Kwon, P Drake, J Williams, B Denison, S Broyer, S Couture, L Nakamura, R Kelsey, MI Krieg, AM Diamond, DJ Zaia, JA AF La Rosa, Corinna Longmate, Jeff Lacey, Simon F. Kaltcheva, Teodora Sharan, Rahul Marsano, Denise Kwon, Peter Drake, Jennifer Williams, Brenda Denison, Sharon Broyer, Suenell Couture, Larry Nakamura, Ryotaro Kelsey, Morris I. Krieg, Arthur M. Diamond, Don J. Zaia, John A. TI Clinical Evaluation of Safety and Immunogenicity of PADRE-Cytomegalovirus (CMV) and Tetanus-CMV Fusion Peptide Vaccines With or Without PF03512676 Adjuvant SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID STEM-CELL TRANSPLANTATION; T-CELLS; IMMUNE-RESPONSES; CPG DNA; TRANSGENIC MICE; INFECTION; RECONSTITUTION; RECIPIENTS; EPITOPES; DISEASE AB Background. It has been reported that cytomegalovirus (CMV) pp65-specific T cells can protect hematopoietic cell transplant (HCT) recipients from CMV complications. Two candidate CMV peptide vaccines composed of the HLA A*0201 pp65495-503 cytotoxic CD8(+) T-cell epitope fused to 2 different universal T-helper epitopes (either the synthetic Pan DR epitope [PADRE] or a natural Tetanus sequence) were clinically evaluated for safety and ability to elicit pp65 T cells in HLA A*0201 healthy volunteers. Methods. Escalating doses (0.5, 2.5, 10 mg) of PADRE or Tetanus pp65(495-503) vaccines with (30 adults) or without (28 adults) PF03512676 adjuvant were administered by subcutaneous injection every 3 weeks for a total of 4 injections. Results. No serious adverse events were reported, although vaccines used in combination with PF03512676 had enhanced reactogenicity. Ex vivo responses were detected by flow cytometry exclusively in volunteers who received the vaccine coadministered with PF03512676. In addition, using a sensitive in vitro stimulation system, vaccine-elicited pp65(495-503) T cells were expanded in 30% of volunteers injected solely with the CMV peptides and in all tested subjects receiving the vaccines coinjected with PF03512676. Conclusions. Acceptable safety profiles and vaccine-driven expansion of pp65(495-503) T cells in healthy adults support further evaluation of CMV peptide vaccines combined with PF03512676 in the HCT setting. Clinical Trials Registration. NCT00722839. C1 [La Rosa, Corinna; Lacey, Simon F.; Kaltcheva, Teodora; Sharan, Rahul; Marsano, Denise; Kwon, Peter; Diamond, Don J.] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Div Translat Vaccine Res, Dept Virol, Duarte, CA 91010 USA. [Longmate, Jeff] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Div Biostat, Dept Informat Sci, Duarte, CA 91010 USA. [Drake, Jennifer; Williams, Brenda] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Gen Clin Res Ctr, Duarte, CA 91010 USA. [Denison, Sharon] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Dept Inpatient Pharm, Duarte, CA 91010 USA. [Broyer, Suenell; Couture, Larry] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Ctr Appl Technol Dev, Duarte, CA 91010 USA. [Nakamura, Ryotaro] City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Dept Hematol & Hematopoiet Cell Transplant, Duarte, CA 91010 USA. [Kelsey, Morris I.] NCI, Dev Therapeut Program, Rapid Access Intervent Dev Program, Frederick, MD 21701 USA. [Krieg, Arthur M.] Atlas Venture, Cambridge, MA USA. RP Diamond, DJ (reprint author), City Hope Comprehens Canc Ctr, Beckman Res Inst City Hope, Div Translat Vaccine Res, Dept Virol, Duarte, CA 91010 USA. EM ddiamond@coh.org OI Longmate, Jeffrey/0000-0002-0869-7928; Diamond, Don/0000-0003-3579-7083 FU NIH, National Cancer Institute [R01-CA77544, P01-CA30206, 24XS044]; NCI-RAID, Division of Cancer Treatment and Diagnosis, National Cancer Institute; National Institute of Allergy and Infectious Diseases [R21 AI084019]; National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R21 DK077374]; NCI [CA33572]; National Center for Research Resources, GCRC; satellite of University of Southern California GCRC [MO1-RR0043-38]; Edwin and Bea Wolfe Charitable Foundation FX This work was supported in part by NIH grants from the following institutes: National Cancer Institute (R01-CA77544 and P01-CA30206, Project III to D. J. D. and contract 24XS044); and also supported in part through the NCI-RAID Program of the Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute; National Institute of Allergy and Infectious Diseases (R21 AI084019 to C. L. R.); National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); R21 DK077374 to S. F. L.); NCI (CA33572 to the COH Comprehensive Cancer Center); National Center for Research Resources (MO1-RR0043-38 to COH General Clinical Research Center [GCRC; satellite of University of Southern California GCRC]); and the Edwin and Bea Wolfe Charitable Foundation to the Division of Translational Vaccine Research. NR 50 TC 29 Z9 29 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 EI 1537-6613 J9 J INFECT DIS JI J. Infect. Dis. PD APR 15 PY 2012 VL 205 IS 8 BP 1294 EP 1304 DI 10.1093/infdis/jis107 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 914VL UT WOS:000301980800017 PM 22402037 ER PT J AU Abaan, OD Davis, SR Bilke, S Polley, EC Walker, RL Pineda, M Zhu, YLJ Reinhold, W Holbeck, SL Simon, RM Doroshow, JH Pommier, Y Meltzer, PS AF Abaan, Ogan D. Davis, Sean R. Bilke, Sven Polley, Eric C. Walker, Robert L. Pineda, Marbin Zhu, Yuelin J. Reinhold, William Holbeck, Susan L. Simon, Richard M. Doroshow, James H. Pommier, Yves Meltzer, Paul S. TI The exomes of the NCI60 and their implications for cancer pharmacogenomics SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Abaan, Ogan D.; Davis, Sean R.; Bilke, Sven; Polley, Eric C.; Walker, Robert L.; Pineda, Marbin; Zhu, Yuelin J.; Reinhold, William; Holbeck, Susan L.; Simon, Richard M.; Doroshow, James H.; Pommier, Yves; Meltzer, Paul S.] NCI, CCR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1879 DI 10.1158/1538-7445.AM2012-1879 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501043 ER PT J AU Abbotts, RM Sultana, R Seedhouse, C Patel, PM Wilson, DM Madhusudan, S AF Abbotts, Rachel M. Sultana, Rebeka Seedhouse, Claire Patel, Poulam M. Wilson, David M. Madhusudan, Srinivasan TI Synthetic lethal targeting of PTEN-associated homologous recombination (HR) deficient melanoma cells by human apurinic/apyrimidinic endonuclease (APE1) inhibitors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Abbotts, Rachel M.; Sultana, Rebeka; Seedhouse, Claire; Patel, Poulam M.; Madhusudan, Srinivasan] Univ Nottingham, Nottingham NG7 2RD, England. [Wilson, David M.] NIA, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-263 DI 10.1158/1538-7445.AM2012-LB-263 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602410 ER PT J AU Achyut, B Bader, DA Pang, YL Harris, CC Yang, L AF Achyut, B. Bader, David A. Pang, Yanli Harris, Curtis C. Yang, Li TI Abrogation of stromal TGF-beta signaling induces DNA damage and genetic/epigenetic alterations in neighboring epithelia SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Achyut, B.; Bader, David A.; Pang, Yanli; Harris, Curtis C.; Yang, Li] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 50 DI 10.1158/1538-7445.AM2012-50 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604437 ER PT J AU Adhikari, AS Sullivan, T Van Dyke, T AF Adhikari, Amit S. Sullivan, Teresa Van Dyke, Terry TI Astrocytoma initiation by Rb family inactivation induced dedifferentiation of mature astrocytes. SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Adhikari, Amit S.; Sullivan, Teresa; Van Dyke, Terry] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3346 DI 10.1158/1538-7445.AM2012-3346 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505102 ER PT J AU Adiseshaiah, PP Stern, ST Patel, NL Ileva, LV Kalen, JD Haines, DC McNeil, SE AF Adiseshaiah, Pavan P. Stern, Stephan T. Patel, Nimit L. Ileva, Lilia V. Kalen, Joseph D. Haines, Diana C. McNeil, Scott E. TI Longitudinal imaging of melanoma cancer cell metastasis in a preclinical model by bioluminescence, PET/CT, and MRI SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Adiseshaiah, Pavan P.; Stern, Stephan T.; Patel, Nimit L.; Ileva, Lilia V.; Kalen, Joseph D.; Haines, Diana C.; McNeil, Scott E.] NCI, SAIC Inc, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-510 DI 10.1158/1538-7445.AM2012-LB-510 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504207 ER PT J AU Alexander, VM Sano, K Yu, ZQ Nakajima, T Choyke, PL Ptaszek, M Kobayashi, H AF Alexander, Vinita M. Sano, Kohei Yu, Zhanqian Nakajima, Takahito Choyke, Peter L. Ptaszek, Marcin Kobayashi, Hisataka TI A GSA-NMP1 conjugate: An NIR-activatable fluorescence imaging agent to detect peritoneal ovarian cancer metastases SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Alexander, Vinita M.; Sano, Kohei; Nakajima, Takahito; Choyke, Peter L.; Kobayashi, Hisataka] NCI, CCR, Bethesda, MD 20892 USA. [Yu, Zhanqian; Ptaszek, Marcin] Univ Maryland Baltimore Cty, Baltimore, MD 21228 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-508 DI 10.1158/1538-7445.AM2012-LB-508 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504204 ER PT J AU Alimova, IN Venkataraman, S Harris, P Marquez, VE Birks, D Foreman, NK Vibhakar, R AF Alimova, Irina N. Venkataraman, Sujatha Harris, Peter Marquez, Victor E. Birks, Diane Foreman, Nicholas K. Vibhakar, Rajeev TI Inhibition of the polycomb group gene EZH2 suppresses growth and radiosensitizes ATRT cells by promoting senescence and inhibiting the CycinD-E2F1 axis SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Alimova, Irina N.; Venkataraman, Sujatha; Harris, Peter; Birks, Diane; Foreman, Nicholas K.; Vibhakar, Rajeev] Univ Colorado Denver, Aurora, CO USA. [Marquez, Victor E.] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2483 DI 10.1158/1538-7445.AM2012-2483 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501478 ER PT J AU Alley, MC Burkett, MW Hite, KM Mertins, SD Niederhuber, JE Shoemaker, RH AF Alley, Michael C. Burkett, Mark W. Hite, Karen M. Mertins, Susan D. Niederhuber, John E. Shoemaker, Robert H. TI Adaptation of soft agar colony formation assays to identify agents which block proliferation of putative colon cancer stem cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Alley, Michael C.; Mertins, Susan D.; Shoemaker, Robert H.] NCI, Dev Therapeut Program, Frederick, MD 21701 USA. [Burkett, Mark W.; Hite, Karen M.] NCI, SAIC Inc, Frederick, MD 21701 USA. [Niederhuber, John E.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3379 DI 10.1158/1538-7445.AM2012-3379 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505347 ER PT J AU Andersen, JB Gillen, M Conner, EA Factor, VM Thorgeirsson, SS AF Andersen, Jesper Boeje Gillen, Matthew Conner, Elisabeth A. Factor, Valentina M. Thorgeirsson, Snorri S. TI Translational genomics analyses of cholangiocarcinoma identify patients who may respond to tyrosine kinase inhibitors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Andersen, Jesper Boeje; Gillen, Matthew; Conner, Elisabeth A.; Factor, Valentina M.; Thorgeirsson, Snorri S.] NCI, CCR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4927 DI 10.1158/1538-7445.AM2012-4927 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604269 ER PT J AU Antony, S Wu, YZ Jiang, GJ Juhasz, A Lu, JM Liu, H Roy, KK Doroshow, JH AF Antony, Smitha Wu, Yongzhong Jiang, Guojian Juhasz, Agnes Lu, Jiamo Liu, Han Roy, Krishnendu K. Doroshow, James H. TI NADPH oxidase NOX5 regulates the expression of the cell cycle inhibitor p27kip1 in the human melanoma UACC-257 cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Antony, Smitha; Wu, Yongzhong; Jiang, Guojian; Juhasz, Agnes; Lu, Jiamo; Liu, Han; Roy, Krishnendu K.; Doroshow, James H.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2060 DI 10.1158/1538-7445.AM2012-2060 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604451 ER PT J AU Aparicio, M Amornphimoltham, P Weigert, R Lewis, J Zudaire, E AF Aparicio, Marta Amornphimoltham, Panomwat Weigert, Roberto Lewis, John Zudaire, Enrique TI Rapid discovery of novel antiangiogenic drugs using a phenotypic profiling platform with high predictive value for preclinical models of cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Aparicio, Marta; Zudaire, Enrique] NCI, NIH, Bethesda, MD 20892 USA. [Amornphimoltham, Panomwat; Weigert, Roberto] NIDCR, NIH, Bethesda, MD USA. [Lewis, John] London Reg Canc Program, Translat Prostate Canc Res Grp, London, ON, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2322 DI 10.1158/1538-7445.AM2012-2322 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504272 ER PT J AU Archer, TK Trotter, KW Singh, A AF Archer, Trevor K. Trotter, Kevin W. Singh, Ajeet TI Chromatin remodeling proteins in transcription and development SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Archer, Trevor K.; Trotter, Kevin W.; Singh, Ajeet] NIEHS, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA SY21-01 DI 10.1158/1538-7445.AM2012-SY21-01 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501081 ER PT J AU Arem, H Bobe, G Sampson, J Subar, AF Park, Y Risch, H Hollenbeck, AR Mayne, ST Stolzenberg-Solomon, R AF Arem, Hannah Bobe, Gerd Sampson, Joshua Subar, Amy F. Park, Yikyung Risch, Harvey Hollenbeck, Albert R. Mayne, Susan T. Stolzenberg-Solomon, Rachael TI Flavonoid intake and risk of pancreatic cancer in the National Institutes of Health-AARP Diet and Health Study Cohort SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Arem, Hannah] Yale Univ, NCI, Bethesda, MD USA. [Bobe, Gerd] Oregon State Univ, Corvallis, OR 97331 USA. [Sampson, Joshua; Subar, Amy F.; Park, Yikyung; Stolzenberg-Solomon, Rachael] NCI, Bethesda, MD 20892 USA. [Risch, Harvey; Mayne, Susan T.] Yale Univ, New Haven, CT USA. [Hollenbeck, Albert R.] AARP, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 625 DI 10.1158/1538-7445.AM2012-625 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602080 ER PT J AU Ashktorab, H Rahi, H Tbaishat, R Daremipouran, M Smoot, DT Lee, EL Varma, S Sun, XG Jia, XY Chung, H Bacon, E Leavitt, R Siegel, P Brim, H AF Ashktorab, Hassan Rahi, Hamed Tbaishat, Rana Daremipouran, Mohammad Smoot, Duane T. Lee, Edward L. Varma, Sudhir Sun, Xueguang Jia, Xi-Yu Chung, Hunter Bacon, Eliza Leavitt, Ron Siegel, Peter Brim, Hassan TI Colorectal cancer, adenoma and normal whole genome methylation sequencing in Africa Americans SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ashktorab, Hassan; Rahi, Hamed; Tbaishat, Rana; Daremipouran, Mohammad; Smoot, Duane T.; Lee, Edward L.; Brim, Hassan] Howard Univ, Washington, DC 20059 USA. [Varma, Sudhir] NIH, Bethesda, MD 20892 USA. [Sun, Xueguang; Jia, Xi-Yu; Chung, Hunter; Bacon, Eliza; Leavitt, Ron] Zymo Res Corp, Riverside, CA USA. [Siegel, Peter] McGill Univ, Rosalind & Morris Goodman Canc Res Ctr, Montreal, PQ, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 994 DI 10.1158/1538-7445.AM2012-994 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501114 ER PT J AU Baas, CL Samson, S AF Baas, Carole L. Samson, Susan TI Advocacy within the PS-OC: Evolution of the program SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Baas, Carole L.] NCI, Irving, TX USA. [Samson, Susan] UCSF, San Francisco, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-58 DI 10.1158/1538-7445.AM2011-LB-58 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600148 ER PT J AU Bahr, JC Robey, R Chakraborty, A Luchenko, V Bates, SE AF Bahr, Julian C. Robey, Robert Chakraborty, Arup Luchenko, Victoria Bates, Susan E. TI Short-term romidepsin treatment combined with mitogen-activated protein kinase and phosphatidylinositol 3-kinase inhibition causes increased Bim expression and cell death in KRAS mutant cell lines SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Bahr, Julian C.; Robey, Robert; Chakraborty, Arup; Luchenko, Victoria; Bates, Susan E.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4708 DI 10.1158/1538-7445.AM2012-4708 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603338 ER PT J AU Banerjee, AA Shen, H Hautman, M Anwer, J Kapetanovic, IM Liu, Y Lyubimov, AV AF Banerjee, Aryamitra A. Shen, Hao Hautman, Mathew Anwer, Jaseem Kapetanovic, Izet M. Liu, Ying Lyubimov, Alexander V. TI Polymeric nanoparticles enhance the bioavailability of the chemopreventive compound SR13668 in beagle dogs SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Banerjee, Aryamitra A.; Shen, Hao; Liu, Ying; Lyubimov, Alexander V.] Univ Illinois, Chicago, IL USA. [Hautman, Mathew; Anwer, Jaseem] Ctr Biomed Testing, Chicago, IL USA. [Kapetanovic, Izet M.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2902 DI 10.1158/1538-7445.AM2012-2902 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600101 ER PT J AU Bassig, B Zhang, LP Cawthon, R Yin, SN Li, GL Rappaport, S Hu, W Smith, MT Rothman, N Vermeulen, R Lan, Q AF Bassig, Bryan Zhang, Luoping Cawthon, Richard Yin, Songnian Li, Guilan Rappaport, Stephen Hu, Wei Smith, Martyn T. Rothman, Nathaniel Vermeulen, Roel Lan, Qing TI Occupational exposure to benzene and leukocyte telomere length SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Bassig, Bryan] Yale Univ, Sch Publ Hlth, New Haven, CT USA. [Zhang, Luoping; Rappaport, Stephen; Smith, Martyn T.] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA. [Cawthon, Richard] Univ Utah, Dept Human Genet, Salt Lake City, UT USA. [Yin, Songnian; Li, Guilan] China CDC, Inst Occupat Hlth & Poison Control, Beijing, Peoples R China. [Hu, Wei; Rothman, Nathaniel; Lan, Qing] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Vermeulen, Roel] Univ Utrecht, Inst Risk Assessment Sci, Utrecht, Netherlands. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4474 DI 10.1158/1538-7445.AM2012-4474 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603413 ER PT J AU Baumbach-Reardon, LL Gomez, C Ahearn, ME Green, A Ellison, K Yariz, KO Issac, B Clarke, J Ambs, S Pegram, M AF Baumbach-Reardon, Lisa L. Gomez, Carmen Ahearn, Mary Ellen Green, Ashley Ellison, Kevin Yariz, Kemal O. Issac, Biju Clarke, Jennifer Ambs, Stefan Pegram, Mark TI Identification and investigation of ethnic specific gene expression differences in non-cancerous breast tissue SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Baumbach-Reardon, Lisa L.] TGEN, Phoenix, AZ USA. [Gomez, Carmen; Ahearn, Mary Ellen; Green, Ashley; Yariz, Kemal O.; Issac, Biju; Clarke, Jennifer; Pegram, Mark] Univ Miami, Sch Med, Miami, FL USA. [Ellison, Kevin] Almac Diagnost, Durham, NC USA. [Ambs, Stefan] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4196 DI 10.1158/1538-7445.AM2012-4196 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502433 ER PT J AU Begaye, A Troste, S Taylor, RE Schriemer, DC Sackett, DL AF Begaye, Adrian Troste, Shana Taylor, Richard E. Schriemer, David C. Sackett, Dan L. TI Localization of the binding site for Peloruside A and Laulimalide on beta-tubulin by physical and biological means SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Begaye, Adrian; Sackett, Dan L.] NICHD, NIH, Bethesda, MD USA. [Troste, Shana] NCI, NIH, Bethesda, MD 20892 USA. [Taylor, Richard E.] Univ Notre Dame, Notre Dame, IN 46556 USA. [Schriemer, David C.] Univ Calgary, Calgary, AB, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2790 DI 10.1158/1538-7445.AM2012-2790 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603271 ER PT J AU Beumer, JH Holleran, JL McCormick, DL Johnson, WD Covey, JM Davis, M Eiseman, JL AF Beumer, Jan H. Holleran, Julianne L. McCormick, David L. Johnson, William D. Covey, Joseph M. Davis, Myrtle Eiseman, Julie L. TI Plasma pharmacokinetics of fluorodeoxycytidine, downstream metabolites, and tetrahydrouridine after combined administration to cynomolgus monkeys SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Beumer, Jan H.; Holleran, Julianne L.; Eiseman, Julie L.] Univ Pittsburgh, Inst Canc, Pittsburgh, PA USA. [McCormick, David L.; Johnson, William D.] IIT, Res Inst, Chicago, IL 60616 USA. [Covey, Joseph M.; Davis, Myrtle] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3782 DI 10.1158/1538-7445.AM2012-3782 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501265 ER PT J AU Bewry, NN AF Bewry, Nadine N. TI Antibody-drug conjugates in oncology drug development: Examining approaches to set the first-in-human dose SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Bewry, Nadine N.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5391 DI 10.1158/1538-7445.AM2012-5391 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500314 ER PT J AU Bilke, S Schwentner, R Fan, Y Kauer, M Walker, RL Kovar, H Melyzer, PS AF Bilke, Sven Schwentner, Raphaela Fan, Yang Kauer, Max Walker, Robert L. Kovar, Heinrich Melyzer, Paul S. TI A functional liaison between E2F and aberrant ETS oncogenes SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Bilke, Sven; Fan, Yang; Walker, Robert L.; Melyzer, Paul S.] NCI, Bethesda, MD 20892 USA. [Schwentner, Raphaela; Kauer, Max; Kovar, Heinrich] St Anna Childrens Hosp, Childrens Canc Res Inst, A-1090 Vienna, Austria. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1285 DI 10.1158/1538-7445.AM2012-1285 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502388 ER PT J AU Blank, M Zhang, YE AF Blank, Michael Zhang, Ying E. TI Smurf2 is a novel tumor suppressor gene that governs chromatin structure and genome integrity through RNF20 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Blank, Michael; Zhang, Ying E.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2195 DI 10.1158/1538-7445.AM2012-2195 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606176 ER PT J AU Bradley, A Heselmeyer-Haddad, K Gaiser, T Lee, WJ Schaffer, AA Andersson, S Ried, T AF Bradley, Amanda Heselmeyer-Haddad, Kerstin Gaiser, Timo Lee, Woei-Jyh Schaffer, Alejandro A. Andersson, Sonia Ried, Thomas TI Clonal patterns of chromosomal aberrations in cervical cancers and precursor lesions SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Bradley, Amanda; Heselmeyer-Haddad, Kerstin; Gaiser, Timo; Ried, Thomas] NCI, Genet Branch, CCR, NIH, Bethesda, MD 20892 USA. [Lee, Woei-Jyh; Schaffer, Alejandro A.] NCBI, NIH, Bethesda, MD USA. [Andersson, Sonia] Karolinska Univ Hosp Solna, Karolinska Inst, Stockholm, Sweden. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2107 DI 10.1158/1538-7445.AM2012-2107 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605235 ER PT J AU Buhrow, SA Kuffel, M Walden, C Reid, JM Covey, J Ames, MM AF Buhrow, Sarah A. Kuffel, Mary Walden, Chad Reid, Joel M. Covey, Joseph Ames, Matthew M. TI Metabolic activation of batracylin and N-acetylbatracylin to reactive metabolites that bind protein and DNA: Possible role in toxicity SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Buhrow, Sarah A.; Kuffel, Mary; Walden, Chad; Reid, Joel M.; Ames, Matthew M.] Mayo Clin, Rochester, MN USA. [Covey, Joseph] NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3769 DI 10.1158/1538-7445.AM2012-3769 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501132 ER PT J AU Byun, JS Gardner, K AF Byun, Jung S. Gardner, Kevin TI Reprogramming the cancer epigenome by "metabolic transduction" SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Byun, Jung S.; Gardner, Kevin] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5019 DI 10.1158/1538-7445.AM2012-5019 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605360 ER PT J AU Cao, Y Lindstrom, S Schumacher, FR Stevens, VL Kraft, P Hsing, AW Ma, J AF Cao, Yin Lindstrom, Sara Schumacher, Fredrick R. Stevens, Victoria L. Kraft, Peter Hsing, Ann W. Ma, Jing CA Breast Prostate Canc Cohort Consor TI IGF gene pathway and progression to fatal prostate cancer after diagnosis in men of European ancestry SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Cao, Yin; Lindstrom, Sara] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Schumacher, Fredrick R.] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. [Stevens, Victoria L.] Amer Canc Soc, Lab Serv, Atlanta, GA 30329 USA. [Kraft, Peter] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Kraft, Peter] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Hsing, Ann W.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Ma, Jing] Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA. [Ma, Jing] Harvard Univ, Sch Med, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2599 DI 10.1158/1538-7445.AM2012-2599 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602223 ER PT J AU Carol, H Lock, R Maris, J Keir, S Gorlick, R Kolb, A Kang, M Reynolds, P Wu, JR Kurmasheva, R Houghton, P Smith, M AF Carol, Hernan Lock, Richard Maris, John Keir, Stephen Gorlick, Richard Kolb, Anders Kang, Min Reynolds, Patrick Wu, Jianrong Kurmasheva, Raushan Houghton, Peter Smith, Malcolm TI Pediatric Preclinical Testing Program (PPTP) evaluation of the JAK inhibitor AZD1480 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Carol, Hernan; Lock, Richard] Childrens Canc Inst Australia, Randwick, NSW, Australia. [Maris, John] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Keir, Stephen] Duke Univ, Med Ctr, Durham, NC USA. [Gorlick, Richard] Montefiore Med Ctr, Bronx, NY 10467 USA. [Kolb, Anders] Alfred I DuPont Hosp Children, Wilmington, DE USA. [Kang, Min; Reynolds, Patrick] Texas Tech Univ, Hlth Sci Ctr, Lubbock, TX 79430 USA. [Wu, Jianrong] St Jude Childrens Res Hosp, Memphis, TN 38105 USA. [Kurmasheva, Raushan; Houghton, Peter] Nationwide Childrens Hosp, Columbus, OH USA. [Smith, Malcolm] NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-318 DI 10.1158/1538-7445.AM2012-LB-318 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504221 ER PT J AU Chang, JT Shebl, FM Pfeiffer, RM Graubard, BI Biryahwaho, B Dollard, SC Mbulaiteye, SM AF Chang, Joanne T. Shebl, Fatma M. Pfeiffer, Ruth M. Graubard, Barry I. Biryahwaho, Benon Dollard, Sheila C. Mbulaiteye, Sam M. TI Investigating human herpesvirus 8 infection among adults in Uganda: A factor analysis approach SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Chang, Joanne T.] Univ Michigan, Natl Canc Inst, Ann Arbor, MI 48109 USA. [Shebl, Fatma M.] Yale Univ, Chron Dis Epidemiol Div, New Haven, CT USA. [Pfeiffer, Ruth M.; Graubard, Barry I.; Mbulaiteye, Sam M.] NCI, DCEG, Bethesda, MD 20892 USA. [Biryahwaho, Benon; Dollard, Sheila C.] Ctr Dis Control & Prevent, Atlanta, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5487 DI 10.1158/1538-7445.AM2012-5487 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602149 ER PT J AU Chang, S Sharan, S AF Chang, Suhwan Sharan, Shyam TI Tumor suppressor BRCA1 epigenetically controls oncogenic miRNA-155 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Chang, Suhwan; Sharan, Shyam] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 142 DI 10.1158/1538-7445.AM2012-142 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503011 ER PT J AU Chanock, SJ AF Chanock, Stephen J. TI Genome-wide association studies in cancer: A step in the right direction SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Chanock, Stephen J.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA SY34-01 DI 10.1158/1538-7445.AM2012-SY34-01 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602200 ER PT J AU Charbonneau, B White, KL Schildkraut, JM Palmieri, RT Iversen, E Berchuck, A Vierkant, RA Rider, DN Cicek, MS Sutphen, R Birrer, MJ Pharoah, PPD Song, HL Tyler, J Gayther, SA Ramus, SJ Wentzensen, N Yang, HP Garcia-Closas, M Phelan, CM Cunningham, JM Fridley, BL Sellers, TA Goode, EL AF Charbonneau, Bridget White, Kristin L. Schildkraut, Joellen M. Palmieri, Rachel T. Iversen, Ed Berchuck, Andrew Vierkant, Robert A. Rider, David N. Cicek, Mine S. Sutphen, Rebecca Birrer, Michael J. Pharoah, Paul P. D. Song, Honglin Tyler, Jonathan Gayther, Simon A. Ramus, Susan J. Wentzensen, Nicolas Yang, Hannah P. Garcia-Closas, Montserrat Phelan, Catherine M. Cunningham, Julie M. Fridley, Brooke L. Sellers, Thomas A. Goode, Ellen L. TI Subtype-specific ovarian cancer risk associated with SNPs in IL1A SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Charbonneau, Bridget; Vierkant, Robert A.; Rider, David N.; Cicek, Mine S.; Cunningham, Julie M.; Fridley, Brooke L.; Goode, Ellen L.] Mayo Clin, Coll Med, Rochester, MN USA. [White, Kristin L.] Columbia Univ, New York, NY USA. [Schildkraut, Joellen M.; Berchuck, Andrew] Duke Univ, Duke Comprehens Canc Ctr, Durham, NC USA. [Palmieri, Rachel T.] Univ N Carolina, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Iversen, Ed] Duke Univ, Dept Stat Sci, Durham, NC USA. [Sutphen, Rebecca] Univ S Florida, Coll Med, Tampa, FL USA. [Birrer, Michael J.] Brigham & Womens Hosp, Boston, MA 02115 USA. [Pharoah, Paul P. D.; Song, Honglin; Tyler, Jonathan] Univ Cambridge, Cambridge, England. [Gayther, Simon A.; Ramus, Susan J.] Univ So Calif, Los Angeles, CA USA. [Wentzensen, Nicolas; Yang, Hannah P.] NCI, Bethesda, MD 20892 USA. [Garcia-Closas, Montserrat] Inst Canc Res, Sutton, Surrey, England. [Sellers, Thomas A.] Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Tampa, FL 33612 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2603 DI 10.1158/1538-7445.AM2012-2603 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602227 ER PT J AU Choi, BH Lee, KS Dai, W AF Choi, Byeong Hyeok Lee, Kyung S. Dai, Wei TI Phosphorylation and regulation of PTEN by Polo-like kinase 1 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Choi, Byeong Hyeok; Dai, Wei] NYU, Dept Environm Med, Langone Med Ctr, Tuxedo Pk, NY USA. [Lee, Kyung S.] NCI, NIH, Tuxedo Pk, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2048 DI 10.1158/1538-7445.AM2012-2048 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604315 ER PT J AU Dahiya, N Bryant, J Aprelikova, O Neiderhuber, J Jazaeri, AA AF Dahiya, Neetu Bryant, Jennifer Aprelikova, Olga Neiderhuber, John Jazaeri, Amir A. TI Comparison of cisplatin induced changes in serous ovarian cancer and normal fallopian tube cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Dahiya, Neetu; Bryant, Jennifer; Jazaeri, Amir A.] Univ Virginia, Charlottesville, VA USA. [Aprelikova, Olga; Neiderhuber, John] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 801 DI 10.1158/1538-7445.AM2012-801 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603053 ER PT J AU Daniel, CR Chow, WH Park, Y Graubard, BII Hollenbeck, AR Sinha, R AF Daniel, Carrie R. Chow, Wong-Ho Park, Yikyung Graubard, Barry I. I. Hollenbeck, Albert R. Sinha, Rashmi TI Intake of fiber and fiber-rich plant foods associated with lower risk of renal cell carcinoma in a large US cohort SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Daniel, Carrie R.; Chow, Wong-Ho; Park, Yikyung; Graubard, Barry I. I.; Sinha, Rashmi] NCI, Rockville, MD USA. [Hollenbeck, Albert R.] AARP, Washington, DC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 659 DI 10.1158/1538-7445.AM2012-659 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602115 ER PT J AU Nguyen, D Witter, S Tomaszewski, JE Ivy, P Doroshow, JH Yang, SX AF Dat Nguyen Witter, Sara Tomaszewski, Joseph E. Ivy, Percy Doroshow, James H. Yang, Sherry X. TI Notch-1 expression in human cancer cell lines and solid tumors by validated immunohistochemistry SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Dat Nguyen; Witter, Sara; Tomaszewski, Joseph E.; Ivy, Percy; Doroshow, James H.; Yang, Sherry X.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 746 DI 10.1158/1538-7445.AM2012-746 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601319 ER PT J AU Davis, M Conlon, KC Bohac, G Barcenas, J Leslie, WT Deng, YP Li, Y Plate, JMD AF Davis, Marcherie Conlon, Kevin C. Bohac, Gerald Barcenas, John Leslie, William T. Deng, Youping Li, Yan Plate, Janet M. D. TI The effects of pemetrexed on innate and adaptive immune cells in subjects with adenocarcinoma of the pancreas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Davis, Marcherie; Barcenas, John; Leslie, William T.; Deng, Youping; Li, Yan; Plate, Janet M. D.] Rush Univ, Med Ctr, Chicago, IL 60612 USA. [Conlon, Kevin C.] NCI, NIH, Bethesda, MD 20892 USA. [Bohac, Gerald] Amgen Inc, Thousand Oaks, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 527 DI 10.1158/1538-7445.AM2012-527 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500426 ER PT J AU De Matteis, S Consonni, D Pesatori, AC Bergen, AW Bertazzi, PA Caporaso, NE Lubin, JH Wacholder, S Landi, MT AF De Matteis, Sara Consonni, Dario Pesatori, Angela C. Bergen, Andrew W. Bertazzi, Pier Alberto Caporaso, Neil E. Lubin, Jay H. Wacholder, Sholom Landi, Maria Teresa TI Tobacco smoking women are not at higher risk than men for lung cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [De Matteis, Sara; Caporaso, Neil E.; Lubin, Jay H.; Wacholder, Sholom; Landi, Maria Teresa] NIH, Rockville, MD USA. [Consonni, Dario; Pesatori, Angela C.; Bertazzi, Pier Alberto] Fdn IRCCS Ca Granda Osped Maggiore Policlin, Milan, Italy. [Consonni, Dario; Pesatori, Angela C.; Bertazzi, Pier Alberto] Univ Milan, Milan, Italy. [Bergen, Andrew W.] SRI Int, Ctr Hlth Sci, Menlo Pk, CA 94025 USA. RI Consonni, Dario/K-7943-2016 OI Consonni, Dario/0000-0002-8935-3843 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 630 DI 10.1158/1538-7445.AM2012-630 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602085 ER PT J AU De Mukhopadhyay, K Elkhaloun, AG Hinck, AP Yoon, K Corneil, JE Yu, L Liu, Z Yang, JH Sun, LZ AF De Mukhopadhyay, Keya Elkhaloun, Abdel G. Hinck, Andrew P. Yoon, Kihoon Corneil, John E. Yu, Lan Liu, Zhao Yang, Junhua Sun, LuZhe TI Delineating the molecular signature of the breast cancer-induced brain metastasis and the role of a novel pan-TGF-beta inhibitor to block brain metastasis in a mouse xenograft model SO CANCER RESEARCH LA English DT Meeting Abstract C1 [De Mukhopadhyay, Keya; Hinck, Andrew P.; Yoon, Kihoon; Corneil, John E.; Yu, Lan; Liu, Zhao; Yang, Junhua; Sun, LuZhe] UT Hlth Sci Ctr, San Antonio, TX USA. [Elkhaloun, Abdel G.] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3390 DI 10.1158/1538-7445.AM2012-3390 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504015 ER PT J AU Demchok, JP Bouchard, B Vaught, J Cho, YM AF Demchok, Joanne P. Bouchard, Brigitte Vaught, Jim Cho, Young-Min TI An international overview of the "state of the science" of regulatory standards for human tissue biobanking SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Demchok, Joanne P.; Vaught, Jim; Cho, Young-Min] NIH, Rockville, MD USA. [Bouchard, Brigitte] Chargee Mission Direct Gen Rech & Innovat, Paris, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4571 DI 10.1158/1538-7445.AM2012-4571 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601249 ER PT J AU Deng, W Dai, CL Zhao, XQ Ohnuma, S Liang, YJ Xiao, ZJ Zeng, MS Ambudkar, SV Fu, LW Chen, ZS AF Deng, Wen Dai, Chun-Ling Zhao, Xiao-qin Ohnuma, Shinobu Liang, Yong-ju Xiao, Zhi-Jie Zeng, Mu-Sheng Ambudkar, Suresh V. Fu, Li-wu Chen, Zhe-Sheng TI Tandutinib (MLN518/CT53518) targeted to stem-like cells by inhibiting the function of ATP-binding cassette subfamily G member 2 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Deng, Wen; Dai, Chun-Ling; Xiao, Zhi-Jie; Chen, Zhe-Sheng] St Johns Univ, Coll Pharm & Allied Hlth Profess, Queens, NY USA. [Zhao, Xiao-qin; Liang, Yong-ju; Zeng, Mu-Sheng; Fu, Li-wu] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou 510275, Guangdong, Peoples R China. [Ohnuma, Shinobu; Ambudkar, Suresh V.] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5614 DI 10.1158/1538-7445.AM2012-5614 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603244 ER PT J AU Du, JH Romano, RA Yang, XP Si, H Bian, YS Sinha, S Van Waes, C Chen, Z AF Du, Jihui Romano, Rose-Anne Yang, Xinping Si, Han Bian, Yansong Sinha, Satrajit Van Waes, Carter Chen, Zhong TI DeltaNp63 overexpression induced cytokines and chemokines attract type2 suppressive inflammatory infiltrates through NF-kappaB activation in dysplastic and malignant epithelia SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Du, Jihui; Yang, Xinping; Si, Han; Bian, Yansong; Van Waes, Carter; Chen, Zhong] NIDCD, NIH, Bethesda, MD USA. [Romano, Rose-Anne; Sinha, Satrajit] SUNY Buffalo, Buffalo, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3549 DI 10.1158/1538-7445.AM2011-3549 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500457 ER PT J AU Dworkin, AM Lee, M Lichtenberg, J Gildea, D Triyedi, N Wolfsberg, T Crawford, NP AF Dworkin, Amy M. Lee, Minnkyong Lichtenberg, Jens Gildea, Derek Triyedi, Niraj Wolfsberg, Tyra Crawford, Nigel P. TI A survival-associated polymorphism within metastasis suppressor RRP1B directs RRP1B-chromatin interactions and self-regulation of gene expression SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Dworkin, Amy M.; Lee, Minnkyong; Lichtenberg, Jens; Gildea, Derek; Triyedi, Niraj; Wolfsberg, Tyra; Crawford, Nigel P.] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3420 DI 10.1158/1538-7445.AM2012-3420 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504048 ER PT J AU Etemadi, A Islami, F van Schooten, FJ Phillips, DH Godschalk, R Golozar, A Kamangar, F Fazel-Tabar, A Pourshams, A Boffetta, P Abnet, CC Malekzadeh, R Dawsey, SM AF Etemadi, Arash Islami, Farhad van Schooten, Frederik-Jan Phillips, David H. Godschalk, Roger Golozar, Asieh Kamangar, Farin Fazel-Tabar, Akbar Pourshams, Akram Boffetta, Paolo Abnet, Christian C. Malekzadeh, Reza Dawsey, Sanford M. TI Genetic determinants of PAH-DNA adduct level and nucleotide excision repair among non-smokers in a high risk area for esophageal squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Etemadi, Arash; Golozar, Asieh; Abnet, Christian C.; Dawsey, Sanford M.] NCI, Bethesda, MD 20892 USA. [Islami, Farhad] Int Agcy Res Canc, F-69372 Lyon, France. [van Schooten, Frederik-Jan; Godschalk, Roger] Maastricht Univ, NL-6200 MD Maastricht, Netherlands. [Phillips, David H.] Inst Canc Res, Sutton, Surrey, England. [Kamangar, Farin] Morgan State Univ, Baltimore, MD 21239 USA. [Fazel-Tabar, Akbar; Pourshams, Akram; Malekzadeh, Reza] Univ Tehran Med Sci, Tehran, Iran. [Boffetta, Paolo] Mt Sinai Sch Med, New York, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2646 DI 10.1158/1538-7445.AM2012-2646 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602275 ER PT J AU Fallon, JK Tighe, R Strittmatter, W Sabzevari, H Schlom, J Greiner, JW AF Fallon, Jonathan K. Tighe, Robert Strittmatter, Wolfgang Sabzevari, Helen Schlom, Jeffrey Greiner, John W. TI A novel immunocytokine generates a CD8+T cell-mediated anti-tumor response in vivo SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Fallon, Jonathan K.; Schlom, Jeffrey; Greiner, John W.] NCI, LTIB, Bethesda, MD 20892 USA. [Tighe, Robert; Strittmatter, Wolfgang; Sabzevari, Helen] EMD Serono, Billerica, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1536 DI 10.1158/1538-7445.AM2012-1536 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500317 ER PT J AU Fang, J AF Fang, Jun TI The processed pseudogene POU5F1P1 in 8q24 is expressed in tumor and shows oncogenicity SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Fang, Jun] NCI, Gaithersburg, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 117 DI 10.1158/1538-7445.AM2012-117 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605392 ER PT J AU Fei, C DeRoo, LA Sandler, DP Weinberg, CR AF Fei, Chunyuan DeRoo, Lisa A. Sandler, Dale P. Weinberg, Clarice R. TI Menopause-associated symptoms, HRT use and risk of young-onset breast cancer: Results from the Two Sister Study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Fei, Chunyuan; DeRoo, Lisa A.; Sandler, Dale P.; Weinberg, Clarice R.] NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5493 DI 10.1158/1538-7445.AM2012-5493 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602155 ER PT J AU Feigelson, HS Bischoff, K Ravel, J Gail, MH Flores, R Goedert, JJ AF Feigelson, Heather Spencer Bischoff, Kimberly Ravel, Jacques Gail, Mrtchell H. Flores, Roberto Goedert, James J. TI Feasibility study of gut microbiome collection in randomly sampled women SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Feigelson, Heather Spencer; Bischoff, Kimberly] Kaiser Permanente, Denver, CO USA. [Ravel, Jacques] Univ Maryland, Baltimore, MD 21201 USA. [Gail, Mrtchell H.; Flores, Roberto; Goedert, James J.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5489 DI 10.1158/1538-7445.AM2012-5489 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602151 ER PT J AU Figueroa, JD Linville, L Brinton, LA Patel, D Clare, SE Visscher, D Mies, C Pfeiffer, RM Gierach, GL Yang, R Hewitt, S Storniolo, AM Sherman, ME AF Figueroa, Jonine D. Linville, Laura Brinton, Louise A. Patel, Deesha Clare, Susan E. Visscher, Daniel Mies, Carrolyn Pfeiffer, Ruth M. Gierach, Gretchen L. Yang, Rose Hewitt, Stephen Storniolo, Anna Maria Sherman, Mark E. TI Breast cancer risk factor associations with breast tissue morphometry: results from the Komen for the Cure (R) Tissue Bank SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Figueroa, Jonine D.; Linville, Laura; Brinton, Louise A.; Patel, Deesha; Pfeiffer, Ruth M.; Gierach, Gretchen L.; Yang, Rose] NCI, DCEG, Bethesda, MD 20892 USA. [Clare, Susan E.; Storniolo, Anna Maria] Indiana Univ, Simon Canc Ctr, Susan G Komen Cure Tissue Bank, Indianapolis, IN 46204 USA. [Visscher, Daniel] Mayo Clin, Ctr Canc, Rochester, MN USA. [Mies, Carrolyn] Univ Penn, Philadelphia, PA 19104 USA. [Hewitt, Stephen; Sherman, Mark E.] NCI, Appl Mol Pathol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4465 DI 10.1158/1538-7445.AM2012-4465 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603313 ER PT J AU Fleming, JL Dworkin, AM Zhang, M Qureshi, AA Allain, DC Fernandez, S Wei, L Peters, S Iwenofu, OH Ridd, K Bastian, BC Han, JL Toland, AE AF Fleming, Jessica L. Dworkin, Amy M. Zhang, Mingfeng Qureshi, Abrar A. Allain, Dawn C. Fernandez, Soledad Wei, Lai Peters, Sara Iwenofu, O. Hans Ridd, Katie Bastian, Boris C. Han, Jiali Toland, Amanda E. TI Allelic-specific imbalance mapping identifies HDAC9 as a candidate susceptibility gene for cutaneous squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Fleming, Jessica L.; Allain, Dawn C.; Fernandez, Soledad; Wei, Lai; Peters, Sara; Iwenofu, O. Hans; Toland, Amanda E.] Ohio State Univ, Columbus, OH 43210 USA. [Dworkin, Amy M.] NIH, Bethesda, MD 20892 USA. [Zhang, Mingfeng; Qureshi, Abrar A.; Han, Jiali] Harvard Univ, Cambridge, MA 02138 USA. [Ridd, Katie; Bastian, Boris C.] Univ Calif San Francisco, San Francisco, CA 94143 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4857 DI 10.1158/1538-7445.AM2012-4857 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501122 ER PT J AU Francis, P Moon, SY Bilke, S Zhu, YLJ Walker, RL Pineda, M Li, XL Abaan, O Knight, J Davis, S Turner, J Wang, H Bruhn, L Lai, A Meltzer, PS AF Francis, Princy Moon, So Young Bilke, Sven Zhu, Yuelin J. Walker, Robert L. Pineda, Marbin Li, Xiao Ling Abaan, Ogan Knight, Jeffrey Davis, Sean Turner, Jaleisa Wang, Hui Bruhn, Laurakay Lai, Ashish Meltzer, Paul S. TI Role of the microRNA-23a similar to 27a similar to 24 clusters in osteosarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Francis, Princy; Moon, So Young; Bilke, Sven; Zhu, Yuelin J.; Walker, Robert L.; Pineda, Marbin; Li, Xiao Ling; Abaan, Ogan; Knight, Jeffrey; Davis, Sean; Turner, Jaleisa; Lai, Ashish; Meltzer, Paul S.] NCI, NIH, Bethesda, MD 20892 USA. [Wang, Hui; Bruhn, Laurakay] Agilent Technol, Agilent Labs, Santa Clara, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1113 DI 10.1158/1538-7445.AM2012-1113 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503037 ER PT J AU Freeman, AK Ritt, DA Morrison, DK AF Freeman, Alyson K. Ritt, Daniel A. Morrison, Deborah K. TI Differential effects of dimerization on B-Raf and C-Raf function revealed by mutational analysis and peptide inhibitors that target the Raf dimer interface SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Freeman, Alyson K.; Ritt, Daniel A.; Morrison, Deborah K.] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1239 DI 10.1158/1538-7445.AM2012-1239 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502226 ER PT J AU Fung, KL Tepede, AK Pluchino, KM Hall, MD Gottesman, MM AF Fung, King Leung Tepede, Abisola K. Pluchino, Kristen M. Hall, Matthew D. Gottesman, Michael M. TI Influx of thiosemicarbazone NSC73306 is mediated by the cisplatin and copper transporter SLC31A1 (CTR1) SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Fung, King Leung; Tepede, Abisola K.; Pluchino, Kristen M.; Hall, Matthew D.; Gottesman, Michael M.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 843 DI 10.1158/1538-7445.AM2012-843 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603160 ER PT J AU Gadd, SL Gerhard, DS Smith, MA Auvil, JG Huff, V Geller, JI Dome, J Huang, CC Perlman, EJ AF Gadd, Samantha L. Gerhard, Daniela S. Smith, Malcom A. Auvil, Jaime Guidry Huff, Vicki Geller, James I. Dome, Jeffrey Huang, Chiang-Ching Perlman, Elizabeth J. TI Loss of expression of SWI/SNF subunits in high risk Wilms tumor is accompanied by global gene expression changes SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gadd, Samantha L.; Huang, Chiang-Ching; Perlman, Elizabeth J.] Northwestern Univ, Chicago, IL 60611 USA. [Gerhard, Daniela S.; Smith, Malcom A.; Auvil, Jaime Guidry] NCI, Bethesda, MD 20892 USA. [Huff, Vicki] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Geller, James I.] Cincinnati Childrens Hosp Med Ctr, Cincinnati, OH 45229 USA. [Dome, Jeffrey] Childrens Natl Med Ctr, Washington, DC 20010 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1439 DI 10.1158/1538-7445.AM2012-1439 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501460 ER PT J AU Gangjee, A Pavana, RK Zhang, X Ihnat, M Hamel, E AF Gangjee, Aleem Pavana, Roheeth Kumar Zhang, Xin Ihnat, Michael Hamel, Ernest TI Design and preclinical evaluation of single agents with antitubulin and antiangiogenic activity as antitumor agents SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gangjee, Aleem; Pavana, Roheeth Kumar; Zhang, Xin] Duquesne Univ, Pittsburgh, PA 15219 USA. [Ihnat, Michael] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. [Hamel, Ernest] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3897 DI 10.1158/1538-7445.AM2012-3897 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601476 ER PT J AU Gangjee, A Lin, L Mooberry, SL Hamel, E AF Gangjee, Aleem Lin, Lu Mooberry, Susan L. Hamel, Ernest TI Substituted pyrrolo[2,3-d]pyrimidines as water soluble microtubule targeting agents SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gangjee, Aleem; Lin, Lu] Duquesne Univ, Grad Sch Pharmaceut Sci, Pittsburgh, PA 15219 USA. [Mooberry, Susan L.] Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA. [Hamel, Ernest] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3889 DI 10.1158/1538-7445.AM2012-3889 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601387 ER PT J AU Gao, L Duan, WR Wu, X Chen, A Otterson, GA Villalona, MA AF Gao, Li Duan, Wenrui Wu, Xin Chen, Alice Otterson, Gregory A. Villalona, Miguel A. TI Compensatory activation of alternative DNA repair pathways following exposure to the PARP Inhibitor ABT-888 in vitro and in patients with solid malignancies SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gao, Li; Duan, Wenrui; Wu, Xin; Otterson, Gregory A.; Villalona, Miguel A.] Ohio State Univ, Columbus, OH 43210 USA. [Chen, Alice] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4682 DI 10.1158/1538-7445.AM2012-4682 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603078 ER PT J AU Gao, Y Caporaso, N AF Gao, Ying Caporaso, Neil TI Gene-specific methylation of leukocyte DNA in relation to colorectal cancer among male smokers from a nested case-control study in the ATBC Study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gao, Ying; Caporaso, Neil] NCI, DCEG, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4467 DI 10.1158/1538-7445.AM2012-4467 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603335 ER PT J AU Garcia-Closas, M Rothman, N Figueroa, JD Prokunina-Olsson, L Han, S Baris, D Jacobs, E Malats, N De Vivo, I Albanes, D Purdue, MP Sharma, S Fu, YP Kogevinas, M Wang, Z Tang, W Tardon, A Serra, C Carrato, A Garcia-Closas, R Lloreta, J Johnson, A Schwenn, M Karagas, MR Schned, A Andriole, G Grubb, R Black, A Gapstur, SM Thun, M Diver, WR Weinstein, SJ Virtamo, J Hunter, DJ Caporaso, N Landi, MT Hutchinson, A Burdett, L Jacobs, KB Yeager, M Fraumeni, JF Chanock, SJ Silverman, DT Chatterjee, N AF Garcia-Closas, M. Rothman, N. Figueroa, J. D. Prokunina-Olsson, L. Han, S. Baris, D. Jacobs, E. Malats, N. De Vivo, I. Albanes, D. Purdue, M. P. Sharma, S. Fu, Y. P. Kogevinas, M. Wang, Z. Tang, W. Tardon, A. Serra, C. Carrato, A. Garcia-Closas, R. Lloreta, J. Johnson, A. Schwenn, M. Karagas, M. R. Schned, A. Andriole, G. Grubb, R. Black, A. Gapstur, S. M. Thun, M. Diver, W. R. Weinstein, S. J. Virtamo, J. Hunter, D. J. Caporaso, N. Landi, M. T. Hutchinson, A. Burdett, L. Jacobs, K. B. Yeager, M. Fraumeni, J. F. Chanock, S. J. Silverman, D. T. Chatterjee, N. TI Synergistic effects of twelve common genetic polymorphisms and smoking habits on absolute risk of bladder cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Garcia-Closas, M.; Sharma, S.] Inst Canc Res, Sutton, Surrey, England. [Rothman, N.; Figueroa, J. D.; Prokunina-Olsson, L.; Han, S.; Baris, D.; Albanes, D.; Purdue, M. P.; Fu, Y. P.; Tang, W.; Black, A.; Weinstein, S. J.; Caporaso, N.; Landi, M. T.; Fraumeni, J. F.; Chanock, S. J.; Silverman, D. T.; Chatterjee, N.] NCI, Bethesda, MD 20892 USA. [Jacobs, E.; Thun, M.; Diver, W. R.] Amer Canc Soc, Atlanta, GA 30329 USA. [Malats, N.] Spanish Natl Canc Res Ctr, Madrid, Spain. [De Vivo, I.] Brigham & Womens Hosp, Boston, MA 02115 USA. [Kogevinas, M.] CREAL, Barcelona, Spain. [Wang, Z.; Hutchinson, A.; Burdett, L.; Jacobs, K. B.; Yeager, M.] NCI, Frederick, MD 21701 USA. [Tardon, A.] Univ Oviedo, Oviedo, Spain. [Serra, C.; Lloreta, J.] Univ Pompeu Fabra, Barcelona, Spain. [Carrato, A.] Ramon y Cajal Univ Hosp, Madrid, Spain. [Garcia-Closas, R.] Hosp Univ Canarias, San Cristobal la Laguna, Spain. [Johnson, A.] Vermont Canc Registry, Burlington, VT USA. [Schwenn, M.] Maine Canc Registy, Augusta, ME USA. [Karagas, M. R.; Schned, A.] Dartmouth Coll, Hitchcock Med Ctr, Dartmouth Med Sch, Hanover, NH 03756 USA. [Andriole, G.; Grubb, R.] Washington Univ, Sch Med, St Louis, MO USA. [Gapstur, S. M.] Amer Canc inst, Atlanta, GA USA. [Virtamo, J.] Natl Inst Hlth & Welf, Helsinki, Finland. [Hunter, D. J.] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. RI Kogevinas, Manolis/C-3918-2017 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-337 DI 10.1158/1538-7445.AM2012-LB-337 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603235 ER PT J AU Gebregiorgis, M Woldemichael, G He, M Kang, MH Nunez, LE Moris, F Smith, MA Helman, LJ Grohar, PJ AF Gebregiorgis, Meti Woldemichael, Girma He, Min Kang, Min H. Nunez, Luz E. Moris, Francisco Smith, Malcolm A. Helman, Lee J. Grohar, Patrick J. TI Identification of mithramycin analogues with increased potency and more selective inhibition of EWS-FLI1 for the treatment of Ewing sarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gebregiorgis, Meti; Helman, Lee J.] NCI, Pediat Oncol Branch, Bethesda, MD 20892 USA. [Woldemichael, Girma] NCI, Mol Targets Lab, Frederick, MD USA. [He, Min] NCI, Dev Therapeut Program, Rockville, MD USA. [Kang, Min H.] Texas Tech Sch Med, Univ Hlth Sci Ctr, Lubbock, TX USA. [Smith, Malcolm A.] NCI, Clin Invest Branch, Rockville, MD USA. [Grohar, Patrick J.] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2494 DI 10.1158/1538-7445.AM2011-2494 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501490 ER PT J AU Gharpure, RV Mendoza, A Dennis, JU Khanna, C AF Gharpure, Radhika V. Mendoza, Arnulfo Dennis, John U. Khanna, Chand TI The PAMM (pulmonary assessment of metastatic morbidity) diagnostic: A novel non-invasive method for evaluating lung metastasis in murine models SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gharpure, Radhika V.; Mendoza, Arnulfo; Khanna, Chand] NCI, Bethesda, MD 20892 USA. [Dennis, John U.] SAIC Frederick Lab Anim Sci Program, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1365 DI 10.1158/1538-7445.AM2012-1365 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504413 ER PT J AU Gibson, TM Engels, EA Clarke, CA Pfeiffer, RM Lynch, CF Chang, ET Hall, E Weisenburger, DD Morton, LM AF Gibson, Todd M. Engels, Eric A. Clarke, Christina A. Pfeiffer, Ruth M. Lynch, Charles F. Chang, Ellen T. Hall, Erin Weisenburger, Dennis D. Morton, Lindsay M. TI Risk of diffuse large B-cell lymphoma in solid organ transplant recipients SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gibson, Todd M.; Engels, Eric A.; Pfeiffer, Ruth M.; Morton, Lindsay M.] NCI, Rockville, MD USA. [Clarke, Christina A.; Chang, Ellen T.] Canc Prevent Inst Calif, Fremont, CA USA. [Lynch, Charles F.] Univ Iowa, Iowa City, IA USA. [Hall, Erin] Johns Hopkins Univ Hosp, Baltimore, MD USA. [Weisenburger, Dennis D.] Univ Nebraska Med Ctr, Omaha, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1025 DI 10.1158/1538-7445.AM2012-1025 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602367 ER PT J AU Godfrey, AC Xu, ZL Weinberg, CR Wade, PA Deroo, LA Sandler, DP Taylor, JA AF Godfrey, Ashley C. Xu, Zongli Weinberg, Clarice R. Wade, Paul A. Deroo, Lisa A. Sandler, Dale P. Taylor, Jack A. TI Serum miRNAs as an early marker for breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Godfrey, Ashley C.; Xu, Zongli; Weinberg, Clarice R.; Wade, Paul A.; Deroo, Lisa A.; Sandler, Dale P.; Taylor, Jack A.] NIEHS, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5049 DI 10.1158/1538-7445.AM2012-5049 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503065 ER PT J AU Gomes-DaGama, EM Moulick, K Ahn, JH Zong, H Rodina, A Cerchietti, L Lopes-Vazquez, MEC Beebe, K Perna, F Katerina, C Vu, LP Zhao, XY Zatorska, D Taldone, T Smith-Jones, P Alpaugh, M Grosso, SS Pillarsetty, N Ku, T Lewis, JS Larson, SM Ross, L Erdjument-Bromage, H Guzman, ML Nimer, SD Melnick, AM Neckers, L Chiosis, G AF Gomes-DaGama, Erica M. Moulick, Kamalika Ahn, James H. Zong, Hongliang Rodina, Anna Cerchietti, Leandro Lopes-Vazquez, Maria E. Caldas Beebe, Kristin Perna, Fabiana Katerina, Chatzi Vu, Ly P. Zhao, Xinyang Zatorska, Danuta Taldone, Tony Smith-Jones, Peter Alpaugh, Mary Grosso, Steven S. Pillarsetty, Nagavarakishore Ku, Thomas Lewis, Jason S. Larson, Steven M. Ross, Levine Erdjument-Bromage, Hediye Guzman, Monica L. Nimer, Stephen D. Melnick, Ari M. Neckers, Len Chiosis, Gabriela TI Affinity-based proteomics reveal cancer-specific networks coordinated by Hsp90 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gomes-DaGama, Erica M.; Moulick, Kamalika; Ahn, James H.; Rodina, Anna; Lopes-Vazquez, Maria E. Caldas; Perna, Fabiana; Vu, Ly P.; Zhao, Xinyang; Zatorska, Danuta; Taldone, Tony; Smith-Jones, Peter; Alpaugh, Mary; Pillarsetty, Nagavarakishore; Ku, Thomas; Lewis, Jason S.; Larson, Steven M.; Ross, Levine; Erdjument-Bromage, Hediye; Nimer, Stephen D.; Chiosis, Gabriela] Mem Sloan Kettering Canc Ctr, New York, NY USA. [Zong, Hongliang; Cerchietti, Leandro; Katerina, Chatzi; Guzman, Monica L.; Melnick, Ari M.] Weill Cornell Med Coll, Div Hematol & Oncol, New York, NY USA. [Beebe, Kristin] NCI, Urol Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Grosso, Steven S.] Cornell Univ, Weill Med Coll, Ithaca, NY 14853 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1263 DI 10.1158/1538-7445.AM2012-1263 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502365 ER PT J AU Gonzalez-Angulo, AM Krop, I Piha-Paul, S Li, YS Culotta, KS Moulder-Thompson, S Tsimberidou, A Velez-Bravo, VM Madden, TL Norberg, LM Doyle, A Winder, EP Mills, GB Meric-Bernstam, F Kurzrock, R AF Gonzalez-Angulo, Ana M. Krop, Ian Piha-Paul, Sarina Li, Yisheng Culotta, Kirk S. Moulder-Thompson, Stacy Tsimberidou, Apostolia Velez-Bravo, Vivianne M. Madden, Timothy L. Norberg, Lisa M. Doyle, Austin Winder, Eric P. Mills, Gordon B. Meric-Bernstam, Funda Kurzrock, Razelle TI Phase lb dose escalation and biomarker study of MK2206 in combination with standard doses of weekly paclitaxel in patients with locally advanced or metastatic solid tumors with expansion in advanced breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gonzalez-Angulo, Ana M.; Piha-Paul, Sarina; Li, Yisheng; Culotta, Kirk S.; Moulder-Thompson, Stacy; Tsimberidou, Apostolia; Velez-Bravo, Vivianne M.; Madden, Timothy L.; Norberg, Lisa M.; Mills, Gordon B.; Meric-Bernstam, Funda; Kurzrock, Razelle] UT MD Anderson Canc Ctr, Houston, TX USA. [Krop, Ian; Winder, Eric P.] Dana Farber Canc Inst, Boston, MA 02115 USA. [Doyle, Austin] NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-231 DI 10.1158/1538-7445.AM2012-LB-231 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601425 ER PT J AU Grade, M Eimer, C Emons, G Kendziorra, E Spitzner, M Gaedcke, J Wolff, H Beissbarth, T Ried, T Pukrop, T Ghadimi, M AF Grade, Marian Eimer, Christine Emons, Georg Kendziorra, Emil Spitzner, Melanie Gaedcke, Jochen Wolff, Hendrik Beissbarth, Tim Ried, Thomas Pukrop, Tobias Ghadimi, Michael TI The Wnt transcription factor TCF4 mediates resistance of colorectal cancer cells to (chemo-) radiotherapy in a beta-catenin independent manner SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Grade, Marian; Eimer, Christine; Emons, Georg; Kendziorra, Emil; Spitzner, Melanie; Gaedcke, Jochen; Wolff, Hendrik; Beissbarth, Tim; Pukrop, Tobias; Ghadimi, Michael] Univ Med Ctr, Gottingen, Germany. [Ried, Thomas] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5729 DI 10.1158/1538-7445.AM2011-5729 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500032 ER PT J AU Gu, F Pfeiffer, R Taylor, P Berndt, S Yang, H Han, S Sigurdson, A Toro, J Mirabello, L Freedman, N Abnet, C Dawsey, S Hu, N Qiao, YL Ding, T Brenner, A Garcia-Closas, M Brinton, L Lissowska, J Wentzensen, N Kratz, C Moore, L Ziegler, R Chow, WH Savage, S Burdette, L Yeager, M Chanock, S Tucker, M Goldstein, A Yang, R AF Gu, Fangyi Pfeiffer, Ruth Taylor, Phil Berndt, Sonja Yang, Hanna Han, Summer Sigurdson, Alice Toro, Jorge Mirabello, Lisa Freedman, Neal Abnet, Christian Dawsey, Sanford Hu, Nan Qiao, You-Lin Ding, Ti Brenner, Alina Garcia-Closas, Montserrat Brinton, Louise Lissowska, Jolanta Wentzensen, Nicolas Kratz, Christian Moore, Lee Ziegler, Regina Chow, Wong-Ho Savage, Sharon Burdette, Laurie Yeager, Meredith Chanock, Stephen Tucker, Margaret Goldstein, Alisa Yang, Rose TI Genetic variants in the 9p21 region in relation to the risk of multiple tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gu, Fangyi; Pfeiffer, Ruth; Taylor, Phil; Berndt, Sonja; Yang, Hanna; Han, Summer; Sigurdson, Alice; Toro, Jorge; Mirabello, Lisa; Freedman, Neal; Abnet, Christian; Dawsey, Sanford; Hu, Nan; Brenner, Alina; Brinton, Louise; Wentzensen, Nicolas; Kratz, Christian; Moore, Lee; Ziegler, Regina; Chow, Wong-Ho; Savage, Sharon; Burdette, Laurie; Yeager, Meredith; Chanock, Stephen; Tucker, Margaret; Goldstein, Alisa; Yang, Rose] NCI, Rockville, MD USA. [Qiao, You-Lin] CICAMS, Beijing, Peoples R China. [Ding, Ti] Shanxi Canc Hosp, Taiyuan, Shanxi, Peoples R China. [Garcia-Closas, Montserrat] Inst Canc Res, Belmont, England. [Lissowska, Jolanta] M Sklodowska Curie Canc Ctr, Warsaw, Poland. RI Qiao, You-Lin/B-4139-2012 OI Qiao, You-Lin/0000-0001-6380-0871 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-330 DI 10.1158/1538-7445.AM2012-LB-330 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603157 ER PT J AU Guedez, L Stetler-Stevenson, WG AF Guedez, Liliana Stetler-Stevenson, William G. TI TIMP-2 inhibits angiogenesis induced by tumor associated myeloid suppressor cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Guedez, Liliana; Stetler-Stevenson, William G.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1376 DI 10.1158/1538-7445.AM2012-1376 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504425 ER PT J AU Gulley, JL Madan, RA Schlom, J AF Gulley, James L. Madan, Ravi A. Schlom, Jeffrey TI Current status of recombinant pox-viral vaccines SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Gulley, James L.; Madan, Ravi A.; Schlom, Jeffrey] NCI, Bethesda, MD 20892 USA. RI Gulley, James/K-4139-2016 OI Gulley, James/0000-0002-6569-2912 NR 12 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA PL03-02 DI 10.1158/1538-7445.AM2012-PL03-02 PG 3 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500211 ER PT J AU Guo, R Morin, P AF Guo, Rong Morin, Patrice TI Identification of signaling pathways involved in the regulation of let-7 in ovarian cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Guo, Rong; Morin, Patrice] NIA, Lab Mol Biol & Immunol, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2310 DI 10.1158/1538-7445.AM2012-2310 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502324 ER PT J AU Guo, ZJ Mitra, R Luo, XH Capdevila, J Lipscomb, J David, PA AF Guo, Zhijun Mitra, Ranjana Luo, Xiangghua Capdevila, Jorge Lipscomb, Johnl David, Potter A. TI CYP3A4 epoxygenase mediates hypoxia-induced biosynthesis of epoxyeicosatrienoic acids (+/-)-11,12-and (+/-)-14,15-EET in breast cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Guo, Zhijun; Luo, Xiangghua; Lipscomb, Johnl; David, Potter A.] Univ Minnesota, Minneapolis, MN USA. [Mitra, Ranjana] NCI, Las Vegas, NV USA. [Capdevila, Jorge] Vanderbilt Univ, Nashville, TN 37235 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 942 DI 10.1158/1538-7445.AM2012-942 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601493 ER PT J AU Hancock, CN Phang, J AF Hancock, Chad N. Phang, James TI The oxidation of proline by proline oxidase provides a regulated source of ROS for mitochondria derived cellular signaling SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hancock, Chad N.; Phang, James] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1119 DI 10.1158/1538-7445.AM2012-1119 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606026 ER PT J AU Harris, M Shin, DM Choi, S Low, B Miller, E Rybinski, B Bronson, R Yun, K AF Harris, Molly Shin, Dong-mi Choi, Seungbum Low, Benjamin Miller, Emily Rybinski, Brad Bronson, Roderick Yun, Kyuson TI Cancer stem cell heterogeneity and cell-of-origin in medulloblastomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Harris, Molly] Univ Maine, Orono, ME USA. [Shin, Dong-mi] NIAID, NIH, Bethesda, MD 20892 USA. [Choi, Seungbum; Low, Benjamin; Miller, Emily; Rybinski, Brad; Yun, Kyuson] Jackson Lab, Ctr Canc, Bar Harbor, ME 04609 USA. [Bronson, Roderick] Dana Farber Canc Inst, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3300 DI 10.1158/1538-7445.AM2012-3300 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504443 ER PT J AU Haso, W Lee, D Morgan, R Pastan, I Mackall, C Orentas, RJ AF Haso, Waleed Lee, Daniel Morgan, Richard Pastan, Ira Mackall, Crystal Orentas, Rimas J. TI Generation and optimization of a chimeric antigen receptor against human CD22: A new immunotherapeutic agent for adoptive immunotherapy SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Haso, Waleed; Lee, Daniel; Morgan, Richard; Pastan, Ira; Mackall, Crystal; Orentas, Rimas J.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3504 DI 10.1158/1538-7445.AM2012-3504 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500377 ER PT J AU Hayman, TJ Bae, H Camphausen, K Tofilon, PJ AF Hayman, Thomas J. Bae, Heekyong Camphausen, Kevin Tofilon, Philip J. TI Competitive inhibition of mTOR results in tumor specific radiosensitization SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hayman, Thomas J.; Bae, Heekyong; Camphausen, Kevin; Tofilon, Philip J.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5727 DI 10.1158/1538-7445.AM2011-5727 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500030 ER PT J AU Heckel, D Padget, M Cherukuri, S Morisot, S Guo, Y Wayne, AS Brown, PA Civin, CI AF Heckel, Diana Padget, Michelle Cherukuri, Srujana Morisot, Sebastien Guo, Yin Wayne, Alan S. Brown, Patrick A. Civin, Curt I. TI Expression of CD22, a late B lymphoid antigen, does not distinguish leukemia stem cells from the bulk population in acute lymphoblastic leukemia cases SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Heckel, Diana; Padget, Michelle; Cherukuri, Srujana; Morisot, Sebastien; Guo, Yin; Civin, Curt I.] Univ Maryland, Sch Med, Dept Pediat, Ctr Stem Cell Biol & Regenerat Med, Baltimore, MD 21201 USA. [Wayne, Alan S.] NCI, Bethesda, MD 20892 USA. [Brown, Patrick A.] Johns Hopkins Univ, Sch Med, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3385 DI 10.1158/1538-7445.AM2012-3385 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505355 ER PT J AU Hesse, JE Innes, C Liu, LW Paules, RS AF Hesse, Jill E. Innes, Cynthia Liu, Liwen Paules, Richard S. TI Genome-wide small RNA sequencing and gene expression analysis reveals a microRNA profile reflective of cancer-susceptibility in ATM deficient human mammary epithelial cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hesse, Jill E.; Innes, Cynthia; Liu, Liwen; Paules, Richard S.] NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-461 DI 10.1158/1538-7445.AM2012-LB-461 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501295 ER PT J AU Hirt, C Meier, M Yu, K Schuler, F Janz, S Dolken, G Rabkin, CS AF Hirt, Carsten Meier, Manuela Yu, Kelly Schueler, Frank Janz, Siegfried Doelken, Gottfried Rabkin, Charles S. TI Population prevalence of consensus translocations in follicular and mantle cell lymphomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hirt, Carsten; Meier, Manuela; Schueler, Frank; Doelken, Gottfried] Ernst Moritz Arndt Univ Greifswald, Greifswald, Germany. [Yu, Kelly; Rabkin, Charles S.] NIH, Rockville, MD USA. [Janz, Siegfried] Univ Iowa, Carver Coll Med, Iowa City, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1672 DI 10.1158/1538-7445.AM2012-1672 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602285 ER PT J AU Hofmann, JN Hosgood, HD Liu, CS Chow, WH Moore, LE Lan, Q Rothman, N Purdue, MP AF Hofmann, Jonathan N. Hosgood, H. Dean Liu, Chin-San Chow, Wong-Ho Moore, Lee E. Lan, Qing Rothman, Nathaniel Purdue, Mark P. TI A prospective study of blood mitochondrial DNA copy number and risk of renal cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hofmann, Jonathan N.; Hosgood, H. Dean; Chow, Wong-Ho; Moore, Lee E.; Lan, Qing; Rothman, Nathaniel; Purdue, Mark P.] NCI, DCEG, Bethesda, MD 20892 USA. [Liu, Chin-San] Changhua Christian Hosp, Changhua, Taiwan. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4461 DI 10.1158/1538-7445.AM2012-4461 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603268 ER PT J AU Hong, B Dicker, DT Kopelovich, L El-Deiry, WS AF Hong, Bo Dicker, David T. Kopelovich, Levy El-Deiry, Wafik S. TI Prodigiosin and its structural analogue rescue deficient p53 pathway signaling and anti-tumor effects via p73 upregulation in p53 mutant and null colorectal cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hong, Bo; Dicker, David T.; El-Deiry, Wafik S.] Penn State Hershey Med Ctr, Penn State Hershey Canc Inst, Hershey, PA USA. [Kopelovich, Levy] NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1172 DI 10.1158/1538-7445.AM2012-1172 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503136 ER PT J AU Horne, HN Sherman, ME Yang, XHR Garcia-Closas, M Lissowska, J Brinton, LA Chanock, SJ Figueroa, JD AF Horne, Hisani N. Sherman, Mark E. Yang, Xiaohong R. Garcia-Closas, Montserrat Lissowska, Jolanta Brinton, Louise A. Chanock, Stephen J. Figueroa, Jonine D. TI Association of variant rs2046210 at 6q25.1 (ESR1) with breast cancer risk suggests heterogeneity by E-cadherin tumor tissue expression SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Horne, Hisani N.; Sherman, Mark E.; Yang, Xiaohong R.; Brinton, Louise A.; Chanock, Stephen J.; Figueroa, Jonine D.] NCI, Bethesda, MD 20892 USA. [Garcia-Closas, Montserrat] Inst Canc Res, Sutton, Surrey, England. [Lissowska, Jolanta] Maria Sklodowska Curie Mem Canc Ctr, Warsaw, Poland. [Lissowska, Jolanta] Inst Oncol, Warsaw, Poland. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-336 DI 10.1158/1538-7445.AM2012-LB-336 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603224 ER PT J AU Hosgood, HD Pao, W Rothman, N Wei, H Kim, C Pan, H Kuchinsky, K Jones, K Xu, J Vermeulen, R Simko, J Lan, Q AF Hosgood, Howard D. Pao, William Rothman, Nathaniel Wei, Hu Kim, Christopher Pan, Helen Kuchinsky, Kyle Jones, Kirk Xu, Jun Vermeulen, Roel Simko, Jeffery Lan, Qing TI Somatic driver mutations among never smoking female lung cancer cases in China identify unique mutation pattern that may be associated with household coal burning SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hosgood, Howard D.; Rothman, Nathaniel; Wei, Hu; Kim, Christopher; Lan, Qing] NCI, Bethesda, MD 20892 USA. [Pao, William; Pan, Helen] Vanderbilt Ingram Canc Ctr, Nashville, TN USA. [Kuchinsky, Kyle; Jones, Kirk; Simko, Jeffery] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Xu, Jun] Univ Hong Kong, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R China. [Vermeulen, Roel] Utrect Univ, Utrecht, Netherlands. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5479 DI 10.1158/1538-7445.AM2012-5479 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602141 ER PT J AU Hu, W Xu, J Bassig, BA Zheng, TZ Zhang, YW Berndt, SI Holford, TR Hosgood, HD Leaderer, B Menashe, I Boyle, P Chanock, S Lan, Q Rothman, N AF Hu, Wei Xu, Jun Bassig, Bryan A. Zheng, Tongzhang Zhang, Yawei Berndt, Sonja I. Holford, Theodore R. Hosgood, H. Dean Leaderer, Brian Menashe, Idan Boyle, Peter Chanock, Stephen Lan, Qing Rothman, Nathaniel TI Polymorphisms in pattern recognition genes in the innate immunity system and risk of non-Hodgkin lymphoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hu, Wei; Bassig, Bryan A.; Berndt, Sonja I.; Hosgood, H. Dean; Menashe, Idan; Chanock, Stephen; Lan, Qing; Rothman, Nathaniel] NCI, Rockville, MD USA. [Xu, Jun] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China. [Zheng, Tongzhang; Zhang, Yawei; Holford, Theodore R.; Leaderer, Brian] Yale Univ, New Haven, CT USA. [Boyle, Peter] Int Prevent Res Inst, Lyon, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2641 DI 10.1158/1538-7445.AM2012-2641 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602270 ER PT J AU Hu, Y Wu, G Rusch, M Lukes, L Buetow, KH Zhang, JH Hunter, KW AF Hu, Ying Wu, Gang Rusch, Michael Lukes, Luanne Buetow, Kenneth H. Zhang, Jinghui Hunter, Kent W. TI An integrated cross-species transcriptional network analysis of metastatic susceptibility SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hu, Ying; Lukes, Luanne; Buetow, Kenneth H.; Hunter, Kent W.] NCI, Bethesda, MD 20892 USA. [Wu, Gang; Rusch, Michael; Zhang, Jinghui] St Jude Childrens Res Hosp, Memphis, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2980 DI 10.1158/1538-7445.AM2012-2980 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505105 ER PT J AU Huang, B Gao, YT Shu, XO Wen, WQ Yang, G Li, GL Ji, BT Li, HL Chow, WH Zheng, W Cai, QY AF Huang, Bo Gao, Yu-Tang Shu, Xiao Ou Wen, Wanqing Yang, Gong Li, Guoliang Ji, Bu-Tian Li, Hong-Lan Chow, Wong Ho Zheng, Wei Cai, Qiuyin TI Mitochondrial DNA copy number and colorectal cancer risk: Results from the Shanghai Women's Health Study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Huang, Bo; Shu, Xiao Ou; Wen, Wanqing; Yang, Gong; Li, Guoliang; Zheng, Wei; Cai, Qiuyin] Vanderbilt Univ, Sch Med, Div Epidemiol, Nashville, TN 37212 USA. [Huang, Bo; Shu, Xiao Ou; Wen, Wanqing; Yang, Gong; Li, Guoliang; Zheng, Wei; Cai, Qiuyin] Vanderbilt Univ, Sch Med, Vanderbilt Epidemiol Ctr, Dept Med, Nashville, TN 37212 USA. [Huang, Bo; Shu, Xiao Ou; Wen, Wanqing; Yang, Gong; Li, Guoliang; Zheng, Wei; Cai, Qiuyin] Vanderbilt Ingram Canc Ctr, Nashville, TN USA. [Gao, Yu-Tang; Li, Hong-Lan] Shanghai Canc Inst, Dept Epidemiol, Shanghai, Peoples R China. [Ji, Bu-Tian; Chow, Wong Ho] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1126 DI 10.1158/1538-7445.AM2012-1126 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606033 ER PT J AU Huppi, K Ou, OL Pitt, JJ Wahlberg, B Jones, TL Rodriguez-Canales, J Erickson, HS Emmert-Buck, M Caplen, NJ AF Huppi, Konrad Ou, Oliver L. Pitt, Jason J. Wahlberg, Brady Jones, Tamara L. Rodriguez-Canales, Jaime Erickson, Heidi S. Emmert-Buck, Michael Caplen, Natasha J. TI Noncoding RNAs of the 8q24 locus SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Huppi, Konrad; Ou, Oliver L.; Pitt, Jason J.; Wahlberg, Brady; Jones, Tamara L.; Rodriguez-Canales, Jaime; Emmert-Buck, Michael; Caplen, Natasha J.] NCI, Bethesda, MD 20892 USA. [Erickson, Heidi S.] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 199 DI 10.1158/1538-7445.AM2012-199 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606138 ER PT J AU Hutt, M Lim, KJ Warren, K Chang, HT Eberhart, CG Raabe, EH AF Hutt, Marianne Lim, Kah Jing Warren, Katherine Chang, Howard T. Eberhart, Charles G. Raabe, Eric H. TI High-level activation of the Notch pathway in diffuse intrinsic pontine glioma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Hutt, Marianne; Lim, Kah Jing; Eberhart, Charles G.] Johns Hopkins Univ, Div Neuropathol, Baltimore, MD USA. [Warren, Katherine] NIH, Pediat Oncol Branch, Bethesda, MD 20892 USA. [Chang, Howard T.] Michigan State Univ, Dept Neurol & Ophthalmol, E Lansing, MI 48824 USA. [Raabe, Eric H.] Johns Hopkins Univ, Div Pediat Oncol, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2480 DI 10.1158/1538-7445.AM2012-2480 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501474 ER PT J AU Iyer, S Xiong, W Tang, DL Jedrychowski, W Chanock, S Wang, S Stigter, L Mroz, E Perera, F AF Iyer, Shoba Xiong, Wei Tang, Deliang Jedrychowski, Wieslaw Chanock, Stephen Wang, Shuang Stigter, Laura Mroz, Elzbieta Perera, Frederica TI Interactions between polycyclic aromatic hydrocarbon exposure and genetic polymorphisms on benzo(a)pyrene-DNA adducts in a Polish cohort of mothers and newborns SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Iyer, Shoba; Xiong, Wei; Tang, Deliang; Wang, Shuang; Stigter, Laura; Perera, Frederica] Columbia Univ, New York, NY USA. [Jedrychowski, Wieslaw; Mroz, Elzbieta] Jagiellonian Univ, Coll Med, Krakow, Poland. [Chanock, Stephen] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2643 DI 10.1158/1538-7445.AM2012-2643 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602272 ER PT J AU Jacobs, KB Yeager, M Zhou, WY Hoover, RN Real, FX Fraumeni, JF Caporaso, NE Tucker, M Rothman, N Perez-Jurado, LA Chanock, SJ AF Jacobs, Kevin B. Yeager, Meredith Zhou, Weiyin Hoover, Robert N. Real, Francisco X. Fraumeni, Joseph F. Caporaso, Neil E. Tucker, Margaret Rothman, Nathaniel Perez-Jurado, Luis A. Chanock, Stephen J. CA DCEG Mosaicism Study Grp TI The aging genome: Genetic mosaicism and its relationship to cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jacobs, Kevin B.; Yeager, Meredith; Zhou, Weiyin] NCI, SAIC Frederick Inc, Frederick, MD 21701 USA. [Hoover, Robert N.; Fraumeni, Joseph F.; Caporaso, Neil E.; Tucker, Margaret; Rothman, Nathaniel; Chanock, Stephen J.] NCI, Rockville, MD USA. [Real, Francisco X.; Perez-Jurado, Luis A.] Univ Pompeu Fabra, Barcelona, Spain. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3110 DI 10.1158/1538-7445.AM2012-3110 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605197 ER PT J AU Jain, M Zhang, LS Joshi, B Puri, R He, M Kebebew, E AF Jain, Meenu Zhang, Lisa Joshi, Bharat Puri, Raj He, Mei Kebebew, Electron TI IL13R alpha 2 regulates cell invasion, and is a therapeutic target for adrenocortical carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jain, Meenu; Zhang, Lisa; He, Mei; Kebebew, Electron] NCI, Bethesda, MD 20892 USA. [Joshi, Bharat; Puri, Raj] US FDA, Bethesda, MD 20014 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3932 DI 10.1158/1538-7445.AM2012-3932 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602167 ER PT J AU Janakiram, NB Mohammed, A Zhang, YT Brewer, M Lightfoot, S Steele, VE Rao, CV AF Janakiram, Naveena B. Mohammed, Altaf Zhang, Yuting Brewer, Misty Lightfoot, Stan Steele, Vernon E. Rao, Chinthalapally V. TI Chemopreventive efficacy of raloxifene, bexarotene and their combination on the progression of AOM-induced colon adenomas to adenocarcinomas in F344 rats SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Janakiram, Naveena B.; Mohammed, Altaf; Zhang, Yuting; Brewer, Misty; Lightfoot, Stan; Rao, Chinthalapally V.] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. [Steele, Vernon E.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1633 DI 10.1158/1538-7445.AM2012-1633 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503370 ER PT J AU Jansen, SA Song, YR O'Sullivan, N Ileva, L Lu, L Van Dyke, T AF Jansen, Sanaz A. Song, Yurong O'Sullivan, Norene Ileva, Lilia Lu, Lucy Van Dyke, Terry TI Modeling glioblastoma in the mouse: Insights from noninvasive imaging SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jansen, Sanaz A.; Song, Yurong; O'Sullivan, Norene; Ileva, Lilia; Lu, Lucy; Van Dyke, Terry] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2441 DI 10.1158/1538-7445.AM2012-2441 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505371 ER PT J AU Jarrett, SG Novak, M Merlino, G Slominski, A Kaetzel, DM AF Jarrett, Stuart G. Novak, Marian Merlino, Glenn Slominski, Andrzej Kaetzel, David M. TI Genome-stabilizing functions of NM23 in metastatic melanoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jarrett, Stuart G.; Novak, Marian; Kaetzel, David M.] Univ Kentucky, Coll Med, Lexington, KY USA. [Jarrett, Stuart G.; Novak, Marian; Kaetzel, David M.] Markey Canc Ctr, Lexington, KY USA. [Merlino, Glenn] NCI, Bethesda, MD 20892 USA. [Slominski, Andrzej] Univ Tennessee, Ctr Hlth Sci, Memphis, TN 38163 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3428 DI 10.1158/1538-7445.AM2012-3428 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504057 ER PT J AU Jenkins, C Stone, S Sobeck, A Wang, WD Hoatlin, ME AF Jenkins, Chelsea Stone, Stacie Sobeck, Alex Wang, Weidong Hoatlin, Maureen E. TI A new protein complex containing the Fanconi protein FANCM and Rif1 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jenkins, Chelsea; Hoatlin, Maureen E.] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Stone, Stacie] Novartis, Portland, OR USA. [Sobeck, Alex] Univ Minnesota, Minneapolis, MN USA. [Wang, Weidong] NIA, NIH, Baltimore, MD 21224 USA. OI Jenkins, Chelsea/0000-0002-9403-0619 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2128 DI 10.1158/1538-7445.AM2012-2128 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605171 ER PT J AU Jiang, Q Xiao, ZX Willette-Brown, J Back, T Wiltrout, RH Hu, YL AF Jiang, Qun Xiao, Zuoxiang Willette-Brown, Jami Back, Timothy Wiltrout, Robert H. Hu, Yinling TI IKK alpha links inflammation and tumorigenesis in a mouse model of lung squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jiang, Qun; Xiao, Zuoxiang; Willette-Brown, Jami; Back, Timothy; Wiltrout, Robert H.; Hu, Yinling] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2561 DI 10.1158/1538-7445.AM2012-2561 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600253 ER PT J AU Jochems, C Tucker, JA Poole, DJ Beatson, M Mulguin, M Madan, RA Figg, WD Dahut, WL Gulley, JL Schlom, J Tsang, KY AF Jochems, Caroline Tucker, Jo A. Poole, Diane J. Beatson, Melony Mulguin, Marcia Madan, Ravi A. Figg, William D. Dahut, William L. Gulley, James L. Schlom, Jeffrey Tsang, Kwong-Yok TI Combination treatment with Bevacizumab, Lenalidomide, Docetaxel and Prednisone (ART -P) does not impact the immune response in patients with metastatic castration -resistant prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jochems, Caroline; Tucker, Jo A.; Poole, Diane J.; Beatson, Melony; Mulguin, Marcia; Madan, Ravi A.; Figg, William D.; Dahut, William L.; Gulley, James L.; Schlom, Jeffrey; Tsang, Kwong-Yok] NCI, Bethesda, MD 20892 USA. RI Gulley, James/K-4139-2016; Figg Sr, William/M-2411-2016 OI Gulley, James/0000-0002-6569-2912; NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5379 DI 10.1158/1538-7445.AM2012-5379 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500300 ER PT J AU Johannessen, L Remsberg, JR Ivanova, A Gaponenko, V Khavrutskii, L Tarasov, SG Dean, M Kates, J Tarasova, NI AF Johannessen, Liv Remsberg, Jarrett R. Ivanova, Alla Gaponenko, Vadim Khavrutskii, Lyuba Tarasov, Sergey G. Dean, Michael Kates, Joseph Tarasova, Nadya I. TI Potent inhibitors of RAS pathways that bind directly to Ras proteins SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Johannessen, Liv; Remsberg, Jarrett R.; Khavrutskii, Lyuba; Tarasov, Sergey G.; Dean, Michael; Tarasova, Nadya I.] NCI, Frederick, MD 21701 USA. [Ivanova, Alla] Vanderbilt Univ, Nashville, TN 37235 USA. [Gaponenko, Vadim] Univ Illinois, Chicago, IL USA. [Kates, Joseph] Calidris Therapeut, Kirkland, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-426 DI 10.1158/1538-7445.AM2012-LB-426 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600237 ER PT J AU Johansson, HE Orjalo, AV Dadhich, S Park, MH AF Johansson, Hans E. Orjalo, Arturo V. Dadhich, Swati Park, Myung Hee TI Hypusine pathway gene expression examined by single molecule RNA fluorescence in situ hybridization (smRNA-FISH) SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Johansson, Hans E.; Orjalo, Arturo V.] Biosearch Technol Inc, Novato, CA USA. [Dadhich, Swati; Park, Myung Hee] NIDCR, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4209 DI 10.1158/1538-7445.AM2012-4209 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502447 ER PT J AU Jube, S Rivera, Z Bianchi, M Powers, A Wang, E Pagano, I Pass, HI Gaudino, G Carbone, M Yang, HN AF Jube, Sandro Rivera, Zeyana Bianchi, Marco Powers, Amy Wang, Ena Pagano, Ian Pass, Harvey I. Gaudino, Giovanni Carbone, Michele Yang, Haining TI High mobility group box 1 secretion supports tumor progression of human malignant mesothelioma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Jube, Sandro; Rivera, Zeyana; Powers, Amy; Pagano, Ian; Gaudino, Giovanni; Carbone, Michele; Yang, Haining] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. [Bianchi, Marco] San Raffaele Univ & Res Inst, Milan, Italy. [Wang, Ena] NIH, Dept Transfus Med, Ctr Clin, Bethesda, MD 20892 USA. [Pass, Harvey I.] NYU, Sch Med, Dept Cardiothorac Surg, New York, NY USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1557 DI 10.1158/1538-7445.AM2012-1557 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500339 ER PT J AU Juhasz, A Antony, S Wu, YZ Lu, JM Jiang, GJ Liu, H Roy, K Doroshow, JH AF Juhasz, Agnes Antony, Smitha Wu, Yongzhong Lu, Jiamo Jiang, Guojian Liu, Han Roy, Krishnendu Doroshow, James H. TI Effect of stable knockdown of NOX1 gene expression with siRNA in human colon cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Juhasz, Agnes; Antony, Smitha; Wu, Yongzhong; Lu, Jiamo; Jiang, Guojian; Liu, Han; Roy, Krishnendu; Doroshow, James H.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3066 DI 10.1158/1538-7445.AM2012-3066 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605092 ER PT J AU Kang, ZG Cao, L AF Kang, Zhigang Cao, Liang TI Genome-wide shRNA screening identifies candidate proteins modulating the extrinsic apoptotic pathway SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kang, Zhigang; Cao, Liang] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 254 DI 10.1158/1538-7445.AM2012-254 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501422 ER PT J AU Kaur, G Kondapaka, SB Teicher, BA AF Kaur, Gurmeet Kondapaka, Sudhir B. Teicher, Beverly A. TI Comparison of endpoints and data analysis methods for exposure of human tumor cells to dasatinib (NSC732517) and 6-MP (NSC755) in culture SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kaur, Gurmeet; Kondapaka, Sudhir B.] NCI, Frederick, MD 21701 USA. [Teicher, Beverly A.] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3713 DI 10.1158/1538-7445.AM2012-3713 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603197 ER PT J AU Kedei, N Telek, A Michalowski, AM Kraft, MB Li, W Poudel, YB Rudra, A Petersen, ME Keck, GE Blumberg, PM AF Kedei, Noemi Telek, Andrea Michalowski, Aleksandra M. Kraft, Matthew B. Li, Wei Poudel, Yam B. Rudra, Arnab Petersen, Mark E. Keck, Gary E. Blumberg, Peter M. TI Comparison of transcriptional response to phorbol ester, bryostatin 1, and bryostatin analogues in LNCaP and U937 cancer cell lines provides insight into their differential mechanism of action SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kedei, Noemi; Telek, Andrea; Michalowski, Aleksandra M.; Blumberg, Peter M.] NCI, Bethesda, MD 20892 USA. [Kraft, Matthew B.; Li, Wei; Poudel, Yam B.; Rudra, Arnab; Petersen, Mark E.; Keck, Gary E.] Univ Utah, Salt Lake City, UT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3815 DI 10.1158/1538-7445.AM2012-3815 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500093 ER PT J AU Kessler, JD Kahle, KT Sun, TT Meerbrey, KL Schlabach, MR Schmitt, EM Skinner, SO Xu, QK Li, MZ Hartman, ZC Rao, M Yu, P Dominguez-Vidana, R Liang, AC Solimin, NL Bernardi, RJ Yu, B Hsu, T Golding, I Luo, J Osborne, CK Creighton, C Hilsenbeck, SG Schiff, R Shaw, CA Elledge, SJ Westbrook, TF AF Kessler, Jessica D. Kahle, Kristopher T. Sun, Tingting Meerbrey, Kristen L. Schlabach, Michael R. Schmitt, Earlene M. Skinner, Samuel O. Xu, Qikai Li, Mamie Z. Hartman, Zachary C. Rao, Mitchell Yu, Peng Dominguez-Vidana, Rocio Liang, Anthony C. Solimin, Nicole L. Bernardi, Ronald J. Yu, Bing Hsu, Tiffany Golding, Ido Luo, Ji Osborne, C. Kent Creighton, Chad Hilsenbeck, Susan G. Schiff, Rachel Shaw, Chad A. Elledge, Stephen J. Westbrook, Thomas F. TI A sumoylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kessler, Jessica D.; Sun, Tingting; Meerbrey, Kristen L.; Schmitt, Earlene M.; Skinner, Samuel O.; Rao, Mitchell; Yu, Peng; Dominguez-Vidana, Rocio; Bernardi, Ronald J.; Hsu, Tiffany; Golding, Ido; Osborne, C. Kent; Creighton, Chad; Hilsenbeck, Susan G.; Schiff, Rachel; Shaw, Chad A.; Westbrook, Thomas F.] Baylor Coll Med, Houston, TX 77030 USA. [Kahle, Kristopher T.; Schlabach, Michael R.; Xu, Qikai; Li, Mamie Z.; Liang, Anthony C.; Solimin, Nicole L.; Elledge, Stephen J.] Harvard Univ, Sch Med, Boston, MA USA. [Hartman, Zachary C.] Duke Univ, Durham, NC USA. [Yu, Bing; Luo, Ji] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3091 DI 10.1158/1538-7445.AM2012-3091 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605118 ER PT J AU Khotskaya, YB Shen, J Chang, SS Yu, DH Steeg, PS Hung, MC AF Khotskaya, Yekaterina B. Shen, Jia Chang, Shih-Shin Yu, Dihua Steeg, Patricia S. Hung, Mien-Chie TI A novel model of breast cancer metastasis: Killing two birds with one stone SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Khotskaya, Yekaterina B.; Shen, Jia; Chang, Shih-Shin; Yu, Dihua; Hung, Mien-Chie] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Steeg, Patricia S.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3290 DI 10.1158/1538-7445.AM2012-3290 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504319 ER PT J AU Kiesel, BF Parise, RA Tjornelund, J Christensen, MK Loza, E Chu, E Kummar, S Beumer, JH AF Kiesel, Brian F. Parise, Robert A. Tjornelund, Jette Christensen, Mette K. Loza, Einars Chu, Edward Kummar, Shivaani Beumer, Jan H. TI Quantitation of the HDAC inhibitor belinostat (PXD-101) and metabolites in human plasma by a novel LC-MS/MS assay. SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kiesel, Brian F.; Parise, Robert A.; Chu, Edward; Beumer, Jan H.] Univ Pittsburgh, Inst Canc, Pittsburgh, PA USA. [Tjornelund, Jette] TopoTarget, Copenhagen, Denmark. [Christensen, Mette K.] Novo Nordisk AS, DK-2880 Bagsvaerd, Denmark. [Loza, Einars] Latvian Inst Organ Synth, Riga, Latvia. [Kummar, Shivaani] NCI, NIH, Bethesda, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 759 DI 10.1158/1538-7445.AM2012-759 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601311 ER PT J AU Kim, C Chapman, R Hu, W He, XZ Hosgood, HD Liu, LZ Lai, H Tian, LW Chen, W Rothman, N Lan, Q AF Kim, Christopher Chapman, Robert Hu, Wei He, Xingzhou Hosgood, H. Dean Liu, Larry Z. Lai, Hong Tian, Linwei Chen, Wei Rothman, Nathaniel Lan, Qing TI Indoor coal smoke exposure, tobacco use, and lung cancer risk in Xuanwei, China SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kim, Christopher; Hu, Wei; Hosgood, H. Dean; Rothman, Nathaniel; Lan, Qing] NIH, Rockville, MD USA. [Chapman, Robert] US EPA, Res Triangle Pk, NC 27711 USA. [He, Xingzhou] Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China. [Liu, Larry Z.] Pfizer, New York, NY USA. [Lai, Hong] Johns Hopkins Univ, Baltimore, MD USA. [Tian, Linwei] Chinese Univ Hong Kong, Ma Liu Shui, Hong Kong, Peoples R China. [Chen, Wei] Forest Labs Inc, New York, NY USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5478 DI 10.1158/1538-7445.AM2012-5478 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602140 ER PT J AU Kim, CJ Cozen, W Weiss, LM Bhatia, K Cockburn, M Hawes, D Wang, SS Engels, EA Morton, LM AF Kim, Clara J. Cozen, Wendy Weiss, Lawrence M. Bhatia, Kishor Cockburn, Myles Hawes, Debra Wang, Sophia S. Engels, Eric A. Morton, Lindsay M. TI Molecular characteristics of diffuse large B-cell lymphoma in HIV-positive and HIV-negative patients SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kim, Clara J.; Bhatia, Kishor; Engels, Eric A.; Morton, Lindsay M.] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Rockville, MD USA. [Cozen, Wendy; Cockburn, Myles; Hawes, Debra] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. [Cozen, Wendy; Cockburn, Myles; Hawes, Debra] Univ So Calif, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA. [Weiss, Lawrence M.] City Hope Natl Med Ctr, Dept Pathol, Duarte, CA 91010 USA. [Wang, Sophia S.] City Hope Natl Med Ctr, Beckman Res Inst, Dept Populat Sci, Div Canc Etiol, Duarte, CA 91010 USA. [Wang, Sophia S.] City Hope Natl Med Ctr, Duarte, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4490 DI 10.1158/1538-7445.AM2012-4490 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602020 ER PT J AU Kim, MKH Annunziata, C AF Kim, Marianne K. H. Annunziata, Christina TI Dual shRNA technique to screen gene-to-gene interaction SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kim, Marianne K. H.; Annunziata, Christina] NCI, NIH, Bethesda, MD 20892 USA. RI Annunziata, Christina/L-3219-2016 OI Annunziata, Christina/0000-0003-2033-6532 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-421 DI 10.1158/1538-7445.AM2012-LB-421 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501282 ER PT J AU Kinyamu, HK Yang, J Archer, TK AF Kinyamu, Harriet K. Yang, Jun Archer, Trevor K. TI LIN28 mRNA targets in breast cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kinyamu, Harriet K.; Yang, Jun; Archer, Trevor K.] NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4192 DI 10.1158/1538-7445.AM2012-4192 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502429 ER PT J AU Kobayashi, H Mitsunaga, M Nakajima, T Sano, K Choyke, PL AF Kobayashi, Hisataka Mitsunaga, Makoto Nakajima, Takahito Sano, Kohei Choyke, Peter L. TI Target-molecular specific near infrared cancer photoimmunotherapy: Detection, treatment, and monitoring of tumors with a theranostic fluorescent probe SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kobayashi, Hisataka; Mitsunaga, Makoto; Nakajima, Takahito; Sano, Kohei; Choyke, Peter L.] NCI, CCR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4830 DI 10.1158/1538-7445.AM2012-4830 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602313 ER PT J AU Kohaar, I Mumy, A Tang, W Porter-Gill, P Fu, YP Prokunina-Olsson, L AF Kohaar, Indu Mumy, Adam Tang, Wei Porter-Gill, Patricia Fu, Yi-Ping Prokunina-Olsson, Ludmila TI Allele-specific effect of rs2294008 on mRNA and protein expression of the prostate stem cell antigen (PSCA) in human normal and tumor bladder tissue SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kohaar, Indu; Mumy, Adam; Tang, Wei; Porter-Gill, Patricia; Fu, Yi-Ping; Prokunina-Olsson, Ludmila] NCI, DCEG, Gaithersburg, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5126 DI 10.1158/1538-7445.AM2012-5126 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606207 ER PT J AU Kosturko, GW Bulut, G Hong, SH Rodriguez, V Brown, M Toretsky, J Khanna, C Paige, M Uren, A AF Kosturko, George W. Bulut, Gulay Hong, Sung-Hyeok Rodriguez, Veronica Brown, Milton Toretsky, Jeffrey Khanna, Chand Paige, Mikell Uren, Aykut TI Design, synthesis and biological evaluation of 2nd generation ezrin inhibitors for metastatic osteosarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kosturko, George W.; Bulut, Gulay; Hong, Sung-Hyeok; Rodriguez, Veronica; Brown, Milton; Toretsky, Jeffrey; Paige, Mikell; Uren, Aykut] Lombardi Comprehens Canc Ctr, Washington, DC USA. [Khanna, Chand] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3906 DI 10.1158/1538-7445.AM2012-3906 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600011 ER PT J AU Truong, K Gouveia, C Coupar, J Smyth, T Lyons, J Chen, Z Van Waes, C AF Kristy Truong Gouveia, Christopher Coupar, Jamie Smyth, Tomoko Lyons, John Chen, Zhong Van Waes, Carter TI Antitumor activity of heat shock protein 90 (HSP90) inhibitor AT13387 in head and neck squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kristy Truong; Gouveia, Christopher; Coupar, Jamie; Chen, Zhong; Van Waes, Carter] NIH, Bethesda, MD 20892 USA. [Smyth, Tomoko; Lyons, John] Astex Pharmaceut, Dublin, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2770 DI 10.1158/1538-7445.AM2012-2770 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603250 ER PT J AU Kwon, HC Kim, SH Oh, SY Lee, S Lee, JH Graves, CA Hwang, JA Hong, SH Kim, HJ Camphausen, K Lee, YS AF Kwon, Hyuk-Chan Kim, Sung-Hyun Oh, Sung Yong Lee, Suee Lee, Ji Hyun Graves, Christian A. Hwang, Jung-Ah Hong, Seung Hyun Kim, Hyo-Jin Camphausen, Kevin Lee, Yeon-Su TI Vascular endothelial growth factor gene polymorphisms and clinical outcome in advanced gastric cancer treated with FOLFOX SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Kwon, Hyuk-Chan; Kim, Sung-Hyun; Oh, Sung Yong; Lee, Suee; Lee, Ji Hyun; Kim, Hyo-Jin] Dong A Univ, Med Ctr, Busan, South Korea. [Graves, Christian A.; Camphausen, Kevin] NCI, Bethesda, MD 20892 USA. [Hwang, Jung-Ah; Hong, Seung Hyun; Lee, Yeon-Su] Natl Canc Ctr, Goyang, South Korea. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4539 DI 10.1158/1538-7445.AM2012-4539 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601029 ER PT J AU Laiyemo, AO Doubeni, C Adebogun, AO McDonald-Pinkett, S Han, P Meissner, HI Klabunde, C AF Laiyemo, Adeyinka O. Doubeni, Chyke Adebogun, Akeem O. McDonald-Pinkett, Shelly Han, Paul Meissner, Helen I. Klabunde, Carrie TI Colorectal cancer screening among US adults with and without doctor's specific recommendation regarding choice of screening modality SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Laiyemo, Adeyinka O.; Adebogun, Akeem O.; McDonald-Pinkett, Shelly] Howard Univ, Washington, DC 20059 USA. [Doubeni, Chyke] Univ Massachusetts, Massachusetts, MA USA. [Han, Paul] Maine Med Ctr Res Inst, Maine, ME USA. [Meissner, Helen I.] NIH, Bethesda, MD 20892 USA. [Klabunde, Carrie] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3565 DI 10.1158/1538-7445.AM2012-3565 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503218 ER PT J AU Lam, TK AF Lam, Tram K. TI Influence of quercetin-rich foods intake on microRNA expression in EAGLE lung cancer tissues SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lam, Tram K.] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2647 DI 10.1158/1538-7445.AM2012-2647 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602276 ER PT J AU Lee, JM Hays, J Annunziata, CM Minasian, L Zujewski, J Squires, J Nielsen, D Houston, N Moorshead, D Cedillo, M Kohn, EC AF Lee, Jung-min Hays, John Annunziata, Christina M. Minasian, Lori Zujewski, JoAnne Squires, Jennifer Nielsen, Deborah Houston, Nicole Moorshead, David Cedillo, Mario Kohn, Elise C. TI A pharmacokinetics/pharmacodynamics study of sequence specificity of the PARP inhibitor, olaparib (O) with carboplatin (C) in recurrent women's cancers NCT01237067 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lee, Jung-min; Hays, John; Annunziata, Christina M.; Minasian, Lori; Zujewski, JoAnne; Squires, Jennifer; Nielsen, Deborah; Houston, Nicole; Moorshead, David; Cedillo, Mario; Kohn, Elise C.] NCI, Bethesda, MD 20892 USA. RI Annunziata, Christina/L-3219-2016 OI Annunziata, Christina/0000-0003-2033-6532 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1754 DI 10.1158/1538-7445.AM2012-1754 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601459 ER PT J AU Lee, M Gildea, D Trivedi, N Wolfsberg, T Crawford, NP AF Lee, Minnkyong Gildea, Derek Trivedi, Niraj Wolfsberg, Tyra Crawford, Nigel P. TI RRP1B, a novel metastasis suppressor, interacts with mRNA splicing factors and regulates alternative mRNA splicing SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lee, Minnkyong; Gildea, Derek; Trivedi, Niraj; Wolfsberg, Tyra; Crawford, Nigel P.] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3423 DI 10.1158/1538-7445.AM2012-3423 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504052 ER PT J AU Lee, YH Bottaro, DP AF Lee, Young H. Bottaro, Donald P. TI Integration of HGF/Met signaling and hypoxia response in cancer cell invasion and proliferation SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lee, Young H.; Bottaro, Donald P.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1225 DI 10.1158/1538-7445.AM2012-1225 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501395 ER PT J AU Leeman-Neilli, RJ Brenner, AV Little, MP Bouville, AC Bogdanova, TI Hatch, M Mabuchi, K Tronko, MD Nikiforov, YE AF Leeman-Neilli, Rebecca J. Brenner, Alina V. Little, Mark P. Bouville, Andre C. Bogdanova, Tetyana I. Hatch, Maureen Mabuchi, Kiyohiko Tronko, Mykola D. Nikiforov, Yuri E. TI Associations between RET/PTC rearrangements, BRAF and RAS mutations and radiation dose, age at exposure, and latency in post-Chernobyl thyroid cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Leeman-Neilli, Rebecca J.; Nikiforov, Yuri E.] Univ Pittsburgh, Sch Med, Pittsburgh, PA USA. [Brenner, Alina V.; Little, Mark P.; Bouville, Andre C.; Hatch, Maureen; Mabuchi, Kiyohiko] NIH, Bethesda, MD 20892 USA. [Bogdanova, Tetyana I.] Inst Endocrinol & Metab, Kiev, Ukraine. [Tronko, Mykola D.] Kiev Univ, Kiev, Ukraine. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2544 DI 10.1158/1538-7445.AM2012-2544 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600234 ER PT J AU Li, HZ Liu, WL Rodriguez-Canales, J Zhu, JQ Hanson, JC Emmert-Buck, MR Deng, CX Rodgers, GP AF Li, Hongzhen Liu, Wenli Rodriguez-Canales, Jaime Zhu, Jianqiong Hanson, Jeffrey C. Emmert-Buck, Michael R. Deng, Chu-Xia Rodgers, Griffin P. TI Olfactomedin 4 gene is associated with progression of prostate cancer: Evidence from an OLFM4 knockout mouse model SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Li, Hongzhen; Liu, Wenli; Zhu, Jianqiong; Rodgers, Griffin P.] NHLBI, NIH, Bethesda, MD 20892 USA. [Rodriguez-Canales, Jaime; Hanson, Jeffrey C.; Emmert-Buck, Michael R.] NCI, NIH, Bethesda, MD 20892 USA. [Deng, Chu-Xia] NIDDK, NIH, Bethesda, MD 20892 USA. RI deng, chuxia/N-6713-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2695 DI 10.1158/1538-7445.AM2012-2695 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601335 ER PT J AU Li, SW Liu, Y Wang, JA Moss, J Darling, TN AF Li, Shaowei Liu, Ying Wang, Ji-an Moss, Joel Darling, Thomas N. TI Increased cathepsin B expression in cultured tuberous sclerosis skin tumor cells and patient tumor tissues SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Li, Shaowei; Liu, Ying; Wang, Ji-an; Darling, Thomas N.] Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. [Moss, Joel] NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2469 DI 10.1158/1538-7445.AM2012-2469 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701506001 ER PT J AU Li, Y Kim, DJ Ma, WY Lubet, R Bode, AM Dong, ZG AF Li, Yan Kim, Dong Joon Ma, Weiya Lubet, Ronald Bode, Ann M. Dong, Zigang TI Discovery of novel checkpoint kinase 1 inhibitors by virtual screening based on multiple crystal structures SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Li, Yan; Kim, Dong Joon; Ma, Weiya; Bode, Ann M.; Dong, Zigang] Univ Minnesota, Hormel Inst, Austin, MN 55912 USA. [Lubet, Ronald] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4780 DI 10.1158/1538-7445.AM2012-4780 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600039 ER PT J AU Li, Y Linnoila, I AF Li, Yan Linnoila, Ilona TI Achaete-scute homolog 1 (Ascl1) lineage in the lung gives rise to multiple cell types SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Li, Yan; Linnoila, Ilona] NCI, Expt Pathol Sect, Cell & Canc Biol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1308 DI 10.1158/1538-7445.AM2012-1308 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502414 ER PT J AU Li, ZB Liu, SL Hassink, M Selvaraj, R Yap, LP Chen, XY Fox, JM Conti, PS AF Li, Zibo Liu, Shuanglong Hassink, Matt Selvaraj, Ramajeyam Yap, Li-peng Chen, Xiaoyuan Fox, Joseph M. Conti, Peter S. TI Tetrazine trans-cyclooctene ligation: An efficient F-18 labeling method for cysteine containing peptides and proteins SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Li, Zibo; Liu, Shuanglong; Yap, Li-peng; Conti, Peter S.] USC, Los Angeles, CA USA. [Hassink, Matt; Selvaraj, Ramajeyam; Fox, Joseph M.] Univ Delaware, Delaware, DE USA. [Chen, Xiaoyuan] NIBIB, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 370 DI 10.1158/1538-7445.AM2012-370 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505407 ER PT J AU Lim, KP Gan, CP Chong, CE Zain, RB Abraham, MT Rahman, ZAA Teo, SH Gutkind, JS Patel, V Ponniah, S Cheong, SC AF Lim, Kue Peng Gan, Chai Phei Chong, Chan Eng Zain, Rosnah Binti Abraham, Mannil Thomas Rahman, Zainal Ariff Abdul Teo, Soo-Hwang Gutkind, J. Silvio Patel, Vyomesh Ponniah, Sathibalan Cheong, Sok Ching TI MAGED4B drives oral carcinogenesis and is a promising peptide vaccine target for the treatment of oral squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lim, Kue Peng; Gan, Chai Phei; Chong, Chan Eng; Teo, Soo-Hwang] Canc Res Initiat Fdn, Subang Jaya, Malaysia. [Zain, Rosnah Binti; Rahman, Zainal Ariff Abdul; Cheong, Sok Ching] Univ Malaya, Kuala Lumpur, Malaysia. [Abraham, Mannil Thomas] Minist Hlth, Kuala Lumpur, Malaysia. [Gutkind, J. Silvio; Patel, Vyomesh] NIH, Bethesda, MD 20892 USA. [Ponniah, Sathibalan] Uniformed Serv Univ Hlth Sci, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1573 DI 10.1158/1538-7445.AM2012-1573 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501014 ER PT J AU Lin, SW Freedman, ND Hu, N Tang, ZZ Wang, LM Wang, CY Ding, T Wang, Y Fan, JH Qiao, YL Wheeler, W Yu, K Goldstein, AM Dawsey, SM Taylor, PR Abnet, CC AF Lin, Shih-Wen Freedman, Neal D. Hu, Nan Tang, Ze-Zhong Wang, Lemin Wang, Chaoyu Ding, Ti Wang, Yuan Fan, Jin-Hu Qiao, You-Lin Wheeler, William Yu, Kai Goldstein, Alisa M. Dawsey, Sanford M. Taylor, Philip R. Abnet, Christian C. TI Genetic variants of iron-dependent metabolism genes and risk of upper gastrointestinal cancers SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lin, Shih-Wen; Freedman, Neal D.; Hu, Nan; Wang, Lemin; Wang, Chaoyu; Yu, Kai; Goldstein, Alisa M.; Dawsey, Sanford M.; Taylor, Philip R.; Abnet, Christian C.] NCI, NIH, Bethesda, MD 20892 USA. [Tang, Ze-Zhong; Ding, Ti; Wang, Yuan] Shanxi Canc Hosp & Inst, Taiyuan, Peoples R China. [Fan, Jin-Hu; Qiao, You-Lin] Chinese Acad Med Sci, Inst Canc, Beijing 100021, Peoples R China. [Wheeler, William] lnformat Management Serv Inc, Silver Spring, MD USA. RI Qiao, You-Lin/B-4139-2012 OI Qiao, You-Lin/0000-0001-6380-0871 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2632 DI 10.1158/1538-7445.AM2012-2632 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602260 ER PT J AU Lin, TL Lee, DW Kochenderfer, JN Mackall, CL AF Lin, Tasha L. Lee, Daniel W. Kochenderfer, James N. Mackall, Crystal L. TI Short-term cytolytic assays are not predictive of chimeric antigen receptor anti-tumor activity mediated by some T cell subsets SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Lin, Tasha L.; Lee, Daniel W.; Kochenderfer, James N.; Mackall, Crystal L.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3502 DI 10.1158/1538-7445.AM2012-3502 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500375 ER PT J AU Liu, T Shi, TJ Hossain, M Schepmoes, A Fillmore, T Sokoll, LJ Chan, D Leach, R Thompson, I Smith, RD Kagan, J Srivastava, S Rodland, KD Camp, DG Qian, WJ AF Liu, Tao Shi, Tujin Hossain, Mahmud Schepmoes, Athena Fillmore, Thomas Sokoll, Lori J. Chan, Daniel Leach, Robin Thompson, Ian Smith, Richard D. Kagan, Jacob Srivastava, Sudhir Rodland, Karin D. Camp, David G. Qian, Wei-Jun TI Accurate measurement of serum total and free PSA using immunoaffinity depletion coupled to SRM: Correlation with clinical immunoassays SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Liu, Tao; Shi, Tujin; Hossain, Mahmud; Schepmoes, Athena; Fillmore, Thomas; Smith, Richard D.; Rodland, Karin D.; Camp, David G.; Qian, Wei-Jun] Pacific NW Natl Lab, Richland, WA 99352 USA. [Sokoll, Lori J.; Chan, Daniel] Johns Hopkins Med Inst, Baltimore, MD 21205 USA. [Leach, Robin; Thompson, Ian] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA. [Kagan, Jacob; Srivastava, Sudhir] NCI, Rockville, MD USA. RI Smith, Richard/J-3664-2012 OI Smith, Richard/0000-0002-2381-2349 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1268 DI 10.1158/1538-7445.AM2012-1268 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502371 ER PT J AU Luhn, P Sherman, M Killian, K Adams, L Stevenson, H Meltzer, P D'Ambrosio, L Hewitt, SM Guido, R Wentzensen, N AF Luhn, Patricia Sherman, Mark Killian, Keith Adams, Lisa Stevenson, Holly Meltzer, Paul D'Ambrosio, Lori Hewitt, Stephen M. Guido, Richard Wentzensen, Nicolas TI Association of endometrial cancer risk factors and DNA methylation in benign endometrial tissue SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Luhn, Patricia; Sherman, Mark; Wentzensen, Nicolas] NCI, Hormonal & Reprod Epidemiol Branch, Div Canc Epidemiol & Genet, Rockville, MD USA. [Killian, Keith; Adams, Lisa; Stevenson, Holly; Meltzer, Paul] NCI, Genet Branch, Ctr Canc Res, Rockville, MD USA. [D'Ambrosio, Lori; Guido, Richard] Magee Womens Hosp, Dept Obstet & Gynecol, Pittsburgh, PA USA. [Hewitt, Stephen M.] NCI, Appl Mol Pathol Lab, Pathol Lab, Ctr Canc Res, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4475 DI 10.1158/1538-7445.AM2012-4475 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603424 ER PT J AU Ma, YW Gupta, V Trieu, R Lap, LT Kaipparettu, BA Wong, LJ AF Ma, Yewei Gupta, Vineet Trieu, Robert Lap, Lee Tin Kaipparettu, Benny A. Wong, Lee-Jun TI Oncogenic potential of mitochondria regulate the cellular tumor characteristics SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ma, Yewei; Gupta, Vineet; Trieu, Robert; Kaipparettu, Benny A.; Wong, Lee-Jun] Baylor Coll Med, Houston, TX 77030 USA. [Lap, Lee Tin] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 998 DI 10.1158/1538-7445.AM2012-998 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501126 ER PT J AU Mancini, M Fer, N Perez, V Teicher, B Rapisarda, A AF Mancini, Monica Fer, Nicole Perez, Victor Teicher, Beverly Rapisarda, Annamaria TI Interleukin 11: A novel therapeutic target in IL-11R alpha expressing sarcomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mancini, Monica; Fer, Nicole; Perez, Victor; Teicher, Beverly; Rapisarda, Annamaria] NCI Frederick, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 890 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604047 ER PT J AU Marincola, FM Wang, E AF Marincola, Francesco M. Wang, Ena TI The immunologic constant of rejection and cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Marincola, Francesco M.; Wang, Ena] NIH CC, Bethesda, MD USA. [Marincola, Francesco M.; Wang, Ena] Trans NIH Ctr Human Immunol, Bethesda, MD USA. NR 9 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA SY32-01 DI 10.1158/1538-7445.AM2012-SY32-01 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500214 ER PT J AU Marino, N Marshall, JCA Collins, J Zhou, M Veenstra, T Steeg, PS AF Marino, Natascia Marshall, Jean-Claude A. Collins, Joshua Zhou, Ming Veenstra, Timothy Steeg, Patricia S. TI Interaction of two metastasis suppressors, Nm23-H1 and Gelsolin, in the proliferation and motility of MDA-MB-231 breast carcinoma cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Marino, Natascia; Marshall, Jean-Claude A.; Collins, Joshua; Steeg, Patricia S.] NCI, Bethesda, MD 20892 USA. [Zhou, Ming; Veenstra, Timothy] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3427 DI 10.1158/1538-7445.AM2012-3427 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504056 ER PT J AU Marrero, AM Lawrence, SM Balasubramanian, P Pommier, YG Tomaszewski, JE Parchment, RE Doroshow, JH Kinders, RJ AF Marrero, Allison M. Lawrence, Scott M. Balasubramanian, Priya Pommier, Yves G. Tomaszewski, Joseph E. Parchment, Ralph E. Doroshow, James H. Kinders, Robert J. TI A multiplex quantitative immunofluorescence assay for DNA damage repair in response to cytotoxic treatment SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Marrero, Allison M.; Lawrence, Scott M.; Balasubramanian, Priya; Parchment, Ralph E.; Kinders, Robert J.] NCI Frederick, Lab Human Toxicol & Pharmacol, Appl Dev Directorate, SAIC Frederick Inc, Frederick, MD USA. [Pommier, Yves G.] NCI, Mol Pharmacol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Tomaszewski, Joseph E.; Doroshow, James H.] NCI, Div Canc Treatment & Diag, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3620 DI 10.1158/1538-7445.AM2012-3620 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600387 ER PT J AU Marshall, JCA Collins, J Zhou, M Veenstra, T Khanna, C Steeg, P AF Marshall, Jean-Claude A. Collins, Joshua Zhou, Ming Veenstra, Timothy Khanna, Chand Steeg, Patricia TI Nm23, a metastasis suppressor gene, binds the cytoskeletal linker Ezrin to regulate motility SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Marshall, Jean-Claude A.; Collins, Joshua; Zhou, Ming; Veenstra, Timothy; Khanna, Chand; Steeg, Patricia] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3421 DI 10.1158/1538-7445.AM2012-3421 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504049 ER PT J AU Matthews, CE Sampson, J Keadle, S Freedson, P Lyden, K Libertine, A Fowke, JH AF Matthews, Charles E. Sampson, Joashua Keadle, Sarah Freedson, Patty Lyden, Kate Libertine, Amanda Fowke, Jay H. TI Evaluation of the performance of a previous day recall of time spent in active and sedentary behaviors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Matthews, Charles E.; Sampson, Joashua] NCI, Bethesda, MD 20892 USA. [Keadle, Sarah; Freedson, Patty; Lyden, Kate; Libertine, Amanda] Univ Massachusetts, Amherst, MA 01003 USA. [Fowke, Jay H.] Vanderbilt Univ, Nashville, TN 37235 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4459 DI 10.1158/1538-7445.AM2012-4459 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503248 ER PT J AU McCollum, AK Angelos, MG Fischione, AD Mineo, M Kohn, EC AF McCollum, Andrea K. Angelos, Mathew G. Fischione, Andrea D. Mineo, Marco Kohn, Elise C. TI A novel function of WW domain binding protein 2 (WBP2) in regulating cytoskeletal function and cellular division through binding to co-chaperone BAG3 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [McCollum, Andrea K.; Angelos, Mathew G.; Fischione, Andrea D.; Mineo, Marco; Kohn, Elise C.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2032 DI 10.1158/1538-7445.AM2012-2032 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604297 ER PT J AU McCully, CM Pastakia, D Bacher, J Thomas, M Steffen-Smith, E Saleem, K Walbridge, S Brinster, L Warren, K AF McCully, Cynthia M. Pastakia, Devang Bacher, John Thomas, Marvin Steffen-Smith, Emilie Saleem, Kadharbatcha Walbridge, Stuart Brinster, Lauren Warren, Katherine TI Development of an animal model for microdialysis sampling of pons and cerebral cortex in rhesus macaques SO CANCER RESEARCH LA English DT Meeting Abstract C1 [McCully, Cynthia M.; Pastakia, Devang; Steffen-Smith, Emilie; Warren, Katherine] NCI, Bethesda, MD 20892 USA. [Bacher, John; Thomas, Marvin; Brinster, Lauren] Off Res Serv, Bethesda, MD USA. [Saleem, Kadharbatcha] NIMH, Bethesda, MD 20892 USA. [Walbridge, Stuart] NINDS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3789 DI 10.1158/1538-7445.AM2012-3789 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501319 ER PT J AU McGlynn, KA Chia, VM Trabert, B Cook, MB Quraishi, S Stanczyk, FZ Rifai, N Bradwin, G Rubertone, MV Graubard, BI Erickson, RL AF McGlynn, Katherine A. Chia, Victoria M. Trabert, Britton Cook, Michael B. Quraishi, Sabah Stanczyk, Frank Z. Rifai, Nader Bradwin, Gary Rubertone, Mark V. Graubard, Barry I. Erickson, Ralph L. TI Pre-diagnostic steroid hormone levels and risk of testicular germ cell tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [McGlynn, Katherine A.; Chia, Victoria M.; Trabert, Britton; Cook, Michael B.; Quraishi, Sabah; Graubard, Barry I.] NCI, Bethesda, MD 20892 USA. [Stanczyk, Frank Z.] Univ So Calif, Los Angeles, CA USA. [Rifai, Nader; Bradwin, Gary] Childrens Hosp Boston, Boston, MA USA. [Rubertone, Mark V.] Dept Def Serum Repository, Silver Spring, MD USA. [Erickson, Ralph L.] Walter Reed Army Inst Res, Silver Spring, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4468 DI 10.1158/1538-7445.AM2012-4468 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603346 ER PT J AU McMaster, ML Heimdal, KR Greene, MH AF McMaster, Mary L. Heimdal, Ketil R. Greene, Mark H. TI No evidence for increased risk of cancers other than testicular cancer among first-degree relatives of testicular germ cell tumor (TGCT) patients from multiple-case TGCT families SO CANCER RESEARCH LA English DT Meeting Abstract C1 [McMaster, Mary L.; Greene, Mark H.] NCI, DCEG, Bethesda, MD 20892 USA. [Heimdal, Ketil R.] Oslo Univ Hosp, Oslo, Norway. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2644 DI 10.1158/1538-7445.AM2012-2644 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602273 ER PT J AU Melenhorst, JJ McIyer, ZA Zheng, HY Wu, CO Grim, A Ito, S Cho, I Hensel, NF Barrett, AJ AF Melenhorst, Jan J. McIyer, Zachariah A. Zheng, Haiyun Wu, Colin O. Grim, Andrew Ito, Sawa Cho, Irene Hensel, Nancy F. Barrett, Austin John TI Low donor thymic output predicts increased incidence of extensive chronic GVHD and worse overall survival after TCD-matched sibling SCT SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Melenhorst, Jan J.; McIyer, Zachariah A.; Zheng, Haiyun; Wu, Colin O.; Grim, Andrew; Ito, Sawa; Cho, Irene; Hensel, Nancy F.; Barrett, Austin John] NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5377 DI 10.1158/1538-7445.AM2012-5377 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500298 ER PT J AU Meshinchi, S Ries, RE Farrar, J Auvil, JG Davidsen, TM Gesuwan, P Trevino, LR Muzny, DM Wheeler, DA Gamis, AS Alonzo, TA Smith, MA Gerhard, DS Arceci, RJ AF Meshinchi, Soheil Ries, Rhonda E. Farrar, Jason Auvil, Jaime Guidry Davidsen, Tanja M. Gesuwan, Patee Trevino, Lisa R. Muzny, Donna M. Wheeler, David A. Gamis, Alan S. Alonzo, Todd A. Smith, Malcolm A. Gerhard, Daniela S. Arceci, Robert J. TI Demonstration of significant clonal evolution from diagnosis to relapse in childhood AML determined by exome capture sequencing: an NCI/COG TARGET AML study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Meshinchi, Soheil; Ries, Rhonda E.] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Farrar, Jason; Arceci, Robert J.] Johns Hopkins Med Inst, Baltimore, MD 21205 USA. [Auvil, Jaime Guidry; Davidsen, Tanja M.; Gesuwan, Patee; Smith, Malcolm A.; Gerhard, Daniela S.] NCI, Bethesda, MD 20892 USA. [Trevino, Lisa R.; Muzny, Donna M.; Wheeler, David A.] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA. [Gamis, Alan S.] Childrens Mercy Hosp & Clin, Kansas City, MO USA. [Alonzo, Todd A.] Univ So Calif, Los Angeles, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-93 DI 10.1158/1538-7445.AM2012-LB-93 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701604189 ER PT J AU Michalowski, AM Simmons, JK Patel, J Kuehl, WM Zhang, SL Landgren, O Mock, BA AF Michalowski, Aleksandra M. Simmons, John K. Patel, Jyoti Kuehl, W. Michael Zhang, Shuling Landgren, Ola Mock, Beverly A. TI Genes cooperatively downregulated by combined mTOR/histone deactylase (HDAC) inhibition are overexpressed in myeloma patients with lower survival SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Michalowski, Aleksandra M.; Simmons, John K.; Patel, Jyoti; Kuehl, W. Michael; Zhang, Shuling; Landgren, Ola; Mock, Beverly A.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4734 DI 10.1158/1538-7445.AM2012-4734 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604032 ER PT J AU Miller, PE Lazarus, P Lesko, SM Cross, AJ Sinha, R Laio, J Zhu, J Harper, G Muscat, JE Hartman, TJ AF Miller, Paige E. Lazarus, Philip Lesko, Samuel M. Cross, Amanda J. Sinha, Rashmi Laio, Jason Zhu, Jay Harper, Gregory Muscat, Joshua E. Hartman, Terryl J. TI Meat-related compounds and colorectal cancer risk by anatomical subsite SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Miller, Paige E.] NCI, Rockville, MD USA. [Lazarus, Philip; Laio, Jason; Zhu, Jay; Muscat, Joshua E.] Penn State Coll Med, Hershey, PA USA. [Lesko, Samuel M.] Northeast Reg Canc Inst, Scranton, PA USA. [Cross, Amanda J.; Sinha, Rashmi] NCI, Bethesda, MD 20892 USA. [Harper, Gregory] Morgan Canc Ctr, Allentown, PA USA. [Hartman, Terryl J.] Penn State Univ, University Pk, PA 16802 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4460A DI 10.1158/1538-7445.AM2012-4460A PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503251 ER PT J AU Mineo, M Taverna, S Flugy, A De Leo, G Alessandro, R Kohn, EC AF Mineo, Marco Taverna, Simona Flugy, Anna De Leo, Giacomo Alessandro, Riccardo Kohn, Elise C. TI Chronic myeloid leukemia (CML) exosomes promote angiogenesis in a Src-dependent fashion in vitro and in vivo SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mineo, Marco; Kohn, Elise C.] NCI, Mol Signaling Sect, Med Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Taverna, Simona; Flugy, Anna; De Leo, Giacomo; Alessandro, Riccardo] Univ Palermo, Dipartimento Biopatol & Biotecnol Med & Forensi, Sez Biol & Genet, Palermo, Italy. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4372 DI 10.1158/1538-7445.AM2012-4372 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502138 ER PT J AU Mirabello, L Chung, C Yeager, MM Savage, SA AF Mirabello, Lisa Chung, Charles Yeager, Meredith M. Savage, Sharon A. TI Understanding cancer associated SNPs in the TERT-CLPTM1L locus through population genetics SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mirabello, Lisa; Savage, Sharon A.] NCI, Div Canc Epidemiol & Genet, NIH, Rockville, MD USA. [Chung, Charles; Yeager, Meredith M.] NCI, Core Genotyping Facil, DCEG, SAIC Frederick Inc, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1668 DI 10.1158/1538-7445.AM2012-1668 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602216 ER PT J AU Mischo, A Harter, P Mueller, K Kleber, S Zachskorn, C Renner, C Steeg, PS Mittelbronn, M Pestalozzi, BC AF Mischo, Axel Harter, Patrick Mueller, Klaus Kleber, Sascha Zachskorn, Cornelia Renner, Christoph Steeg, Patricia S. Mittelbronn, Michel Pestalozzi, Bernhard C. TI Docetaxel pretreatment is associated with increased incidence of CNS-metastases in a murine model of HER2-positive breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mischo, Axel; Kleber, Sascha; Renner, Christoph; Pestalozzi, Bernhard C.] Univ Zurich Hosp, CH-8091 Zurich, Switzerland. [Harter, Patrick; Mueller, Klaus; Zachskorn, Cornelia; Mittelbronn, Michel] Edinger Inst, Neurol Inst, Frankfurt, Germany. [Steeg, Patricia S.] NCI, Ctr Canc Res, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1395 DI 10.1158/1538-7445.AM2012-1395 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503483 ER PT J AU Mishra, PJ Guo, T Zaidi, R Davis, S Arnheiter, H Walker, RL Meltzer, P Merlino, G AF Mishra, Pravin J. Guo, Theresa Zaidi, Raza Davis, Sean Arnheiter, Heinz Walker, Robert L. Meltzer, Paul Merlino, Glenn TI Using embryonic melanoblast transcriptome analysis to identify novel mechanisms promoting metastatic melanoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Davis, Sean; Walker, Robert L.; Meltzer, Paul] NCI, Lab Canc Biol & Genet, NIH, Bethesda, MD 20892 USA. [Arnheiter, Heinz] NINDS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4856 DI 10.1158/1538-7445.AM2012-4856 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501120 ER PT J AU Mkrtichyan, M Najjar, YG Raulfs, EC Liu, LD Langerman, S Khleif, SN AF Mkrtichyan, Mikayel Najjar, Yana G. Raulfs, Estella C. Liu, Linda Langerman, Solomon Khleif, Samir N. TI PD-1 expression level on T cell subsets is a crucial factor forming a novel mechanism for designing immune therapeutic approaches using PD-1 binding ligand SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mkrtichyan, Mikayel; Najjar, Yana G.; Raulfs, Estella C.; Khleif, Samir N.] NCI, NIH, Bethesda, MD 20892 USA. [Liu, Linda; Langerman, Solomon] Amplimmune Inc, Gaithersburg, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-491 DI 10.1158/1538-7445.AM2012-LB-491 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504245 ER PT J AU Mohammed, A Janakiram, NB Brewer, M Biddick, L Lighffoot, S Steele, VE Rao, CV AF Mohammed, Altaf Janakiram, Naveena B. Brewer, Misty Biddick, Laura Lighffoot, Stan Steele, Vernon E. Rao, Chinthalapally V. TI Targeting COX-LOX and EGFR pathways simultaneously by licofelone and gefitinib lead to complete blockade of progression of PanINs to pancreatic ductal adenocarcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mohammed, Altaf; Janakiram, Naveena B.; Brewer, Misty; Biddick, Laura; Lighffoot, Stan; Rao, Chinthalapally V.] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. [Steele, Vernon E.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1005 DI 10.1158/1538-7445.AM2012-1005 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503178 ER PT J AU Moiola, CP De Luca, P Zalazar, F Cotignola, J Gardner, K Vazquez, E De Siervi, A AF Moiola, Cristian P. De Luca, Paola Zalazar, Florencia Cotignola, Javier Gardner, Kevin Vazquez, Elba De Siervi, Adriana TI ATM transcriptional regulation mediated by BRCA1/E2F1 axis controls DNA damage response in prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Moiola, Cristian P.; De Luca, Paola; Zalazar, Florencia; Cotignola, Javier; Vazquez, Elba; De Siervi, Adriana] Univ Buenos Aires, Dept Biol Chem, Buenos Aires, DF, Argentina. [Gardner, Kevin] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1312 DI 10.1158/1538-7445.AM2012-1312 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502419 ER PT J AU Mondul, AM Weinstein, SJ Bosworth, T Remaley, AT Virtamo, J Albanes, D AF Mondul, Alison M. Weinstein, Stephanie J. Bosworth, Tracey Remaley, Alan T. Virtamo, Jarmo Albanes, Demetrius TI Circulating thyroxine (T4), thyroid-stimulating hormone (TSH), and hypothyroid status and the risk of prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Mondul, Alison M.; Weinstein, Stephanie J.; Albanes, Demetrius] NCI, DCEG, Rockville, MD USA. [Bosworth, Tracey; Remaley, Alan T.] NCI, Ctr Clin, Bethesda, MD 20892 USA. [Virtamo, Jarmo] Natl Inst Hlth & Welf, Helsinki, Finland. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4472 DI 10.1158/1538-7445.AM2012-4472 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603390 ER PT J AU Monks, A Hose, CD Hayete, B Runge, K DeCaprio, D Teicher, BA Khalil, I McDonaugh, P Doroshow, JH AF Monks, Anne Hose, Curtis D. Hayete, Boris Runge, Karl DeCaprio, David Teicher, Beverley A. Khalil, Iya McDonaugh, Paul Doroshow, James H. TI Confirmation of peroxiredoxin II as a driver gene for doxorubicin sensitivity identified from drug-induced expression profiling of the NCI-60 cell lines using Reverse Engineering (REFS) network models SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Monks, Anne; Hose, Curtis D.] NCI, Frederick, MD 21701 USA. [Hayete, Boris; Runge, Karl; DeCaprio, David; Khalil, Iya; McDonaugh, Paul] GNS Healthcare, Cambridge, MA USA. [Teicher, Beverley A.; Doroshow, James H.] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5663 DI 10.1158/1538-7445.AM2012-5663 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603488 ER PT J AU Montrose, DC Kopelovich, L Zhou, XK Yantiss, R Subbaramaiah, K Dannenberg, A AF Montrose, David C. Kopelovich, Levy Zhou, Xi Kathy Yantiss, Rhonda Subbaramaiah, Kotha Dannenberg, Andrew TI Metabolomic profiling for early detection of colorectal neoplasia SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Montrose, David C.; Zhou, Xi Kathy; Yantiss, Rhonda; Subbaramaiah, Kotha; Dannenberg, Andrew] Weill Cornell Med Coll, New York, NY USA. [Kopelovich, Levy] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1634 DI 10.1158/1538-7445.AM2012-1634 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503372 ER PT J AU Moody, TW Mantey, S Jensen, RT Wink, D Mukhopadhyay, P Pacher, P AF Moody, Terry W. Mantey, Samuel Jensen, Robert T. Wink, David Mukhopadhyay, Partha Pacher, Pal TI Cannabinoids inhibit epidermal growth factor receptor transactivation in lung cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Moody, Terry W.; Wink, David] NCI, CCR, Bethesda, MD 20892 USA. [Mantey, Samuel; Jensen, Robert T.] NIDDK, Bethesda, MD 20892 USA. [Mukhopadhyay, Partha; Pacher, Pal] NIAAA, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1091 DI 10.1158/1538-7445.AM2012-1091 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605458 ER PT J AU Moon, SY Bilke, S Davis, S Walker, RL Pineda, M Zhu, YJ Abaan, O Meltzer, PS AF Moon, So Young Bilke, Sven Davis, Sean Walker, Robert L. Pineda, Marbin Zhu, Yuelin Jack Abaan, Ogan Meltzer, Paul S. TI Paired-end RNA-sequencing reveals novel fusion genes and SNVs in osteosarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Moon, So Young; Bilke, Sven; Davis, Sean; Walker, Robert L.; Pineda, Marbin; Zhu, Yuelin Jack; Abaan, Ogan; Meltzer, Paul S.] NCI, NIH, CCR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5070 DI 10.1158/1538-7445.AM2012-5070 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606274 ER PT J AU Morrison, BL Bernal, F AF Morrison, Bethanie L. Bernal, Federico TI Modulation of metastatic behavior in breast cancers harboring p53 mutations SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Morrison, Bethanie L.; Bernal, Federico] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4329 DI 10.1158/1538-7445.AM2012-4329 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501438 ER PT J AU Morrow, JJ Briggs, J Ren, L Chakrabarti, K Cassavaugh, J Veenstra, TD Chen, QR Khan, J Uren, A Khanna, C AF Morrow, James J. Briggs, Joseph Ren, Ling Chakrabarti, Kristi Cassavaugh, Jessica Veenstra, Timothy D. Chen, Qingrong Khan, Javed Uren, Aykut Khanna, Chand TI Ezrin plays a key role in the regulation of translation in metastatic osteosarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Morrow, James J.] NIH, Howard Hughes Med Inst, Bethesda, MD 20892 USA. [Briggs, Joseph; Ren, Ling; Chakrabarti, Kristi; Cassavaugh, Jessica; Khanna, Chand] NCI, Tumor & Metastasis Biol Sect, Pediat Oncol Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. [Veenstra, Timothy D.] NCI, Lab Prote & Analyt Technol, Adv Technol Program, SAIC Frederick Inc, Frederick, MD 21701 USA. [Chen, Qingrong; Khan, Javed] NCI, Oncogen Sect, Pediat Oncol Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. [Uren, Aykut] Georgetown Univ, Med Ctr, Lombardi Comprehens Canc Ctr, Washington, DC 20007 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4190 DI 10.1158/1538-7445.AM2012-4190 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502427 ER PT J AU Murthy, SRK Lee, TK Cawley, NX Hewitt, SM Pacak, K Loh, P AF Murthy, Saravana R. K. Lee, Terence K. Cawley, Niamh X. Hewitt, Stephen M. Pacak, Karel Loh, Peng TI An N-terminal truncated carboxypeptidase E splice isoform induces metastasis by activating nedd9 and other metastasis inducing genes SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Murthy, Saravana R. K.; Cawley, Niamh X.; Pacak, Karel; Loh, Peng] NICHD, NIH, Bethesda, MD USA. [Lee, Terence K.] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China. [Hewitt, Stephen M.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5327 DI 10.1158/1538-7445.AM2012-5327 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504101 ER PT J AU Musselman, J Bergemann, T Krailo, M Malkin, D Ross, J Savage, S Nagarajan, R Sklar, C Spector, L AF Musselman, Jessica Bergemann, Tracy Krailo, Mark Malkin, David Ross, Julie Savage, Sharon Nagarajan, Rajaram Sklar, Charles Spector, Logan TI A case-parent-triad approach in assessing risk of osteosarcoma associated with genetic variation in insulin-like growth factor 1/growth hormone axis genes: a Children's Oncology Group (COG) study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Musselman, Jessica; Ross, Julie; Spector, Logan] Univ Minnesota, Minneapolis, MN USA. [Bergemann, Tracy] Medtronic, Minneapolis, MN USA. [Krailo, Mark] Univ So Calif, Los Angeles, CA USA. [Malkin, David] Univ Toronto, Toronto, ON, Canada. [Savage, Sharon] NCI, Washington, DC USA. [Nagarajan, Rajaram] Univ Cincinnati, Cincinnati, OH USA. [Sklar, Charles] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-333 DI 10.1158/1538-7445.AM2012-LB-333 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603191 ER PT J AU Myung, K AF Myung, Kyungjae TI High -throughput genotoxicity screening unlocks chemotherapeutic potential of antioxidants SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Myung, Kyungjae] NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2838 DI 10.1158/1538-7445.AM2012-2838 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604020 ER PT J AU Naing, A LoRusso, P Subbiah, V Fu, SQ Hong, D Anderson, P Benjamin, R Ludwig, J Chen, HX Doyle, A Kurzrock, R AF Naing, Aung LoRusso, Patricia Subbiah, Vivek Fu, Siqing Hong, David Anderson, Peter Benjamin, Robert Ludwig, Joseph Chen, Helen X. Doyle, Austin Kurzrock, Razelle TI Insulin growth factor receptor (IGF-1R) antibody cixutumumab combined with the mTOR inhibitor temsirolimus in patients with refractory Ewing's sarcoma family tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Naing, Aung; Subbiah, Vivek; Fu, Siqing; Hong, David; Anderson, Peter; Benjamin, Robert; Ludwig, Joseph; Kurzrock, Razelle] UT MD Anderson Canc Ctr, Houston, TX USA. [LoRusso, Patricia] Karmanos Canc Inst, Detroit, MI USA. [Chen, Helen X.; Doyle, Austin] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-124 DI 10.1158/1538-7445.AM2012-LB-124 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601401 ER PT J AU Nasser, MW Qamri, Z Deol, YS Ravi, J Powell, CA Trikha, P Schwendener, RA Shilo, K Wolf, R Yuspa, SH Ganju, RK AF Nasser, Mohd W. Qamri, Zahida Deol, Yadwinder S. Ravi, Janani Powell, Catherine A. Trikha, Prashant Schwendener, Reto A. Shilo, Konstantin Wolf, Ronald Yuspa, Stuart H. Ganju, Ramesh K. TI mS100a7a15 enhances mammary tumor growth and metastasis by recruiting tumor associated macrophages SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Nasser, Mohd W.; Qamri, Zahida; Deol, Yadwinder S.; Ravi, Janani; Powell, Catherine A.; Trikha, Prashant; Shilo, Konstantin; Ganju, Ramesh K.] Ohio State Univ, Columbus, OH 43210 USA. [Schwendener, Reto A.] Univ Zurich, Zurich, Switzerland. [Wolf, Ronald] Univ Munich, Munich, Germany. [Yuspa, Stuart H.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 388 DI 10.1158/1538-7445.AM2012-388 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505442 ER PT J AU Newton, DL Stockwin, LH Mullendore, ME Han, BN Morrison, BL Borgel, S Stotler, H Fang, BL Hollingshead, MG AF Newton, Dianne L. Stockwin, Luke H. Mullendore, Michael E. Han, Bingnan Morrison, Bethanie L. Borgel, Suzanne Stotler, Howard Fang, Bingliang Hollingshead, Melinda G. TI NSC 743380 induces apoptosis in sensitive cell lines through activation of tyrosine kinase 2 (Tyk2) SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Newton, Dianne L.; Stockwin, Luke H.; Mullendore, Michael E.; Han, Bingnan; Morrison, Bethanie L.] NCI, Drug Mech Grp, SAIC Frederick Inc, Frederick, MD 21701 USA. [Borgel, Suzanne; Stotler, Howard] NCI, In Vivo Preclin Support Grp, SAIC Frederick Inc, Frederick, MD 21701 USA. [Fang, Bingliang] Univ Texas MD Anderson Canc Ctr, Dept Thorac & Cardiovasc Surg, Houston, TX 77030 USA. [Hollingshead, Melinda G.] NCI, Biol Testing Branch, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2810 DI 10.1158/1538-7445.AM2012-2810 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603472 ER PT J AU Niedan, S Kauer, M Jug, G Walker, RL Meltzer, PS Kontny, U Kovar, H AF Niedan, Stephan Kauer, Max Jug, Gunhild Walker, Robert L. Meltzer, Paul S. Kontny, Udo Kovar, Heinrich TI Reactivation of EWS-FLI1 suppressed FOXO1 expression as a novel therapeutic strategy for Ewing's sarcoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Niedan, Stephan; Kauer, Max; Jug, Gunhild; Kovar, Heinrich] St Anna Childrens Hosp, Childrens Canc Res Inst, A-1090 Vienna, Austria. [Walker, Robert L.; Meltzer, Paul S.] NCI, Genet Branch, Bethesda, MD USA. [Kontny, Udo] Univ Freiburg Klinikum, Freiburg, Germany. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2961 DI 10.1158/1538-7445.AM2012-2961 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501142 ER PT J AU O'Konek, J Takao, S Izhak, L Illarinov, P Besra, G Berzofsky, J Terabe, M AF O'Konek, Jessica Takao, Satomi Izhak, Liat Illarinov, Petr Besra, Gurdyal Berzofsky, Jay Terabe, Masaki TI alpha-mannosylceramide, a new NKT cell agonist, induces tumor immunity without anergy induction in NKT cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [O'Konek, Jessica; Takao, Satomi; Izhak, Liat; Berzofsky, Jay; Terabe, Masaki] NCI, Bethesda, MD 20892 USA. [Illarinov, Petr; Besra, Gurdyal] Univ Birmingham, Birmingham, W Midlands, England. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1556 DI 10.1158/1538-7445.AM2012-1556 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500338 ER PT J AU Ou, O Huppi, K Gehlhaus, K Jones, T Caplen, N AF Ou, Oliver Huppi, Konrad Gehlhaus, Kristen Jones, Tamara Caplen, Natasha TI Large-scale RNAi screening of human kinome identifies putative breast cancer related molecular targets SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ou, Oliver; Huppi, Konrad; Gehlhaus, Kristen; Jones, Tamara; Caplen, Natasha] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 267 DI 10.1158/1538-7445.AM2012-267 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502328 ER PT J AU Pantoja, DRS Masaki, T Ridnour, LA DeGraff, W Berzofsky, JA Roberts, DD AF Pantoja, David R. Soto Masaki, Terabe Ridnour, Lisa A. DeGraff, William Berzofsky, Jay A. Roberts, David D. TI Lack of CD47 in the tumor microenvironment enhances anti-tumor adaptive immune responses when combined with ionizing radiation SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Pantoja, David R. Soto; Masaki, Terabe; Ridnour, Lisa A.; DeGraff, William; Berzofsky, Jay A.; Roberts, David D.] NCI, NIH, Bethesda, MD 20892 USA. RI Roberts, David/A-9699-2008 OI Roberts, David/0000-0002-2481-2981 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3451 DI 10.1158/1538-7445.AM2012-3451 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502019 ER PT J AU Park, H Readio, N Jin, G Asfaha, S Singh, A Singh, A Yang, XD Patterson, KS Trempus, C Wang, TC Morris, RJ AF Park, Heuijoon Readio, Nyssa Jin, Guang Asfaha, Samuel Singh, Anupama Singh, Ashok Yang, Xiangdong Patterson, Kelly S. Trempus, Carol Wang, Timothy C. Morris, Rebecca J. TI Bone marrow-derived epithelial cells contribute to chronic skin inflammation and skin tumor formation in the mouse SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Park, Heuijoon; Jin, Guang; Asfaha, Samuel; Yang, Xiangdong; Patterson, Kelly S.; Wang, Timothy C.] Columbia Univ, New York, NY USA. [Readio, Nyssa; Singh, Anupama; Singh, Ashok; Morris, Rebecca J.] Univ Minnesota, Hormel Inst, Austin, MN 55912 USA. [Trempus, Carol] NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5202 DI 10.1158/1538-7445.AM2012-5202 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505065 ER PT J AU Park, SR Kinders, RJ Khin, S Hollingshead, M Parchment, RE Tomaszewski, JE Doroshow, JH AF Park, Sook Ryun Kinders, Robert J. Khin, Sonny Hollingshead, Melinda Parchment, Ralph E. Tomaszewski, Joseph E. Doroshow, James H. TI Validation and fitness testing of a quantitative immunoassay for HIF1 alpha in biopsy specimens SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Park, Sook Ryun; Tomaszewski, Joseph E.; Doroshow, James H.] NCI, Div Canc Treatment & Diag, Bethesda, MD 20892 USA. [Kinders, Robert J.; Khin, Sonny; Parchment, Ralph E.] NCI Frederick, Lab Human Toxicol & Pharmacol, SAIC Frederick Inc, Frederick, MD USA. [Hollingshead, Melinda] NCI Frederick, Biol Testing Branch, Dev Therapeut Program, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3616 DI 10.1158/1538-7445.AM2012-3616 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600383 ER PT J AU Peng, XJ Yuan, L Fatouma, A Mehta, R Kopelovich, L McCormick, DL AF Peng, Xinjian Yuan, Liang Fatouma, Alimirah Mehta, Rajendra Kopelovich, Levy McCormick, David L. TI Synergistic antiproliferative activity of combined administration of atorvastatin and zoledronic acid in LNCaP and PC3 prostate cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Peng, Xinjian; Yuan, Liang; Fatouma, Alimirah; Mehta, Rajendra; McCormick, David L.] IIT, Res Inst, Chicago, IL 60616 USA. [Kopelovich, Levy] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 613 DI 10.1158/1538-7445.AM2012-613 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503307 ER PT J AU Permuth-Wey, J Lin, HY Tsai, YY Chen, ZH Chen, YA Barnholtz-Sloan, J Birrer, MJ Chanock, S Cramer, DW Cunningham, JM Fenstermacher, D Fridley, B Garcia-Closas, M Gayther, SA Gentry-Maharaj, A Gonzalez-Bosquet, J Iversen, E Jim, H McLaughlin, J Menon, U Monteiro, A Narod, SA Phelan, CM Ramus, S Risch, H Song, H Sutphen, R Terry, KL Tyrer, J Vierkant, RA Wentzensen, N Lancaster, JM Cheng, JQ Berchuk, A Pharoah, PDP Schildkraut, JM Goode, EL Sellers, TA AF Permuth-Wey, Jennifer Lin, Hui-Yi Tsai, Ya-Yu Chen, Zhihua Chen, Y. Ann Barnholtz-Sloan, Jill Birrer, Micheal J. Chanock, Stephen Cramer, Daniel W. Cunningham, Julie M. Fenstermacher, David Fridley, Brooke Garcia-Closas, Montserrat Gayther, Simon A. Gentry-Maharaj, Alexandra Gonzalez-Bosquet, Jesus Iversen, Edwin Jim, Heather McLaughlin, John Menon, Usha Monteiro, Alvaro Narod, Steven A. Phelan, Catherine M. Ramus, Susan Risch, Harvey Song, Honglin Sutphen, Rebecca Terry, Kathryn L. Tyrer, Jonathan Vierkant, Robert A. Wentzensen, Nicolas Lancaster, Johnathan M. Cheng, Jin Q. Berchuk, Andrew Pharoah, Paul D. P. Schildkraut, Joellen M. Goode, Ellen L. Sellers, Thomas A. TI MicroRNA binding site polymorphisms influence ovarian cancer risk in the collaborative oncological gene-environment study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Permuth-Wey, Jennifer; Lin, Hui-Yi; Tsai, Ya-Yu; Chen, Zhihua; Chen, Y. Ann; Fenstermacher, David; Gonzalez-Bosquet, Jesus; Jim, Heather; Monteiro, Alvaro; Phelan, Catherine M.; Lancaster, Johnathan M.; Cheng, Jin Q.; Sellers, Thomas A.] Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Tampa, FL 33612 USA. [Barnholtz-Sloan, Jill] Case Comprehens Canc Ctr, Cleveland, OH USA. [Birrer, Micheal J.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Chanock, Stephen; Wentzensen, Nicolas] NIH, Rockville, MD USA. [Cramer, Daniel W.; Terry, Kathryn L.] Brigham Womens Hosp, Boston, MA USA. [Cunningham, Julie M.; Fridley, Brooke; Vierkant, Robert A.; Goode, Ellen L.] Mayo Clin, Coll Med, Rochester, MN USA. [Garcia-Closas, Montserrat] Inst Canc Res, London SW3 6JB, England. [Garcia-Closas, Montserrat] Breakthrough Breast Canc Res Ctr, London, England. [Gayther, Simon A.; Ramus, Susan] Univ So Calif, Los Angeles, CA USA. [Gentry-Maharaj, Alexandra; Menon, Usha] UCL, EGA Inst Womens Hlth, London, England. [Iversen, Edwin] Duke Univ, Dept Stat Sci, Durham, NC USA. [McLaughlin, John] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada. [Narod, Steven A.] Ctr Res Womens Hlth, Toronto, ON, Canada. [Risch, Harvey] Yale Univ, Sch Med, New Haven, CT USA. [Song, Honglin; Tyrer, Jonathan; Pharoah, Paul D. P.] Univ Cambridge, Cambridge, England. [Sutphen, Rebecca] Univ S Florida, Tampa, FL USA. [Berchuk, Andrew; Schildkraut, Joellen M.] Duke Univ, Med Ctr, Durham, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2927 DI 10.1158/1538-7445.AM2012-2927 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602478 ER PT J AU Pommier, YG Murai, J Takeda, S AF Pommier, Yves G. Murai, Junko Takeda, Shunichi TI Stabilization of PARP-DNA complexes plays a critical role for the cytotoxic effects of the PARP inhibitors, veliparib and oliparib SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Pommier, Yves G.; Murai, Junko] NCI, CCR, Bethesda, MD 20892 USA. [Takeda, Shunichi] Kyoto Med Univ, Kyoto, Japan. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4689 DI 10.1158/1538-7445.AM2012-4689 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603086 ER PT J AU Poplini, EA August, DA Ben-Menachem, T Michael, H Artymyshyn, R Gulley, JL Schlom, J DiPaola, RS Lattime, EC AF Poplini, Elizabeth A. August, David A. Ben-Menachem, Tamir Michael, Hazar Artymyshyn, Renee Gulley, James L. Schlom, Jeffrey DiPaola, Robert S. Lattime, Edmund C. TI Phase I trial of EUS-guided intratumoral vaccination with recombinant Panvac-F and systemic Panvac-V in patients with locally advanced pancreatic cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Poplini, Elizabeth A.; August, David A.; Ben-Menachem, Tamir; Michael, Hazar; Artymyshyn, Renee; DiPaola, Robert S.; Lattime, Edmund C.] Canc Inst New Jersey, New Brunswick, NJ USA. [Gulley, James L.; Schlom, Jeffrey] NCI, Bethesda, MD 20892 USA. RI Gulley, James/K-4139-2016 OI Gulley, James/0000-0002-6569-2912 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5378 DI 10.1158/1538-7445.AM2012-5378 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500299 ER PT J AU Poulikakos, PI Persaud, Y Janakiraman, M Kong, XJ Ng, C Moriceau, G Shi, HB Atefi, M Titz, B Gabay, MT Salton, M Dahlman, KB Tadi, M Wargo, JA Flaherty, KT Kelley, MC Misteli, T Chapman, PB Sosman, JA Graeber, TG Ribas, A Lo, RS Rosen, N Solit, DB AF Poulikakos, Poulikos I. Persaud, Yogindra Janakiraman, Manickam Kong, Xiangju Ng, Charles Moriceau, Gatien Shi, Hubing Atefi, Mohammad Titz, Bjoern Gabay, May Tal Salton, Maayan Dahlman, Kimberly B. Tadi, Madhavi Wargo, Jennifer A. Flaherty, Keith T. Kelley, Mark C. Misteli, Tom Chapman, Paul B. Sosman, Jeffrey A. Graeber, Thomas G. Ribas, Antoni Lo, Roger S. Rosen, Neal Solit, David B. TI RAS-independent dimerization of BRAF(V600E) splicing variants promotes resistance to RAF inhibitors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Poulikakos, Poulikos I.; Persaud, Yogindra; Janakiraman, Manickam; Gabay, May Tal; Tadi, Madhavi; Chapman, Paul B.; Rosen, Neal; Solit, David B.] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. [Kong, Xiangju; Ng, Charles; Moriceau, Gatien; Shi, Hubing; Atefi, Mohammad; Titz, Bjoern; Graeber, Thomas G.; Ribas, Antoni; Lo, Roger S.] Univ Calif Los Angeles, Los Angeles, CA USA. [Salton, Maayan; Misteli, Tom] NCI, NIH, Bethesda, MD 20892 USA. [Dahlman, Kimberly B.; Kelley, Mark C.; Sosman, Jeffrey A.] Vanderbilt Ingram Canc Ctr, Nashville, TN USA. [Wargo, Jennifer A.; Flaherty, Keith T.] Massachusetts Gen Hosp, Ctr Canc, Boston, MA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-428 DI 10.1158/1538-7445.AM2012-LB-428 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501094 ER PT J AU Prabhu, VV Hong, B Allen, JE Dicker, DT Navaraj, A Kopelovich, L El-Deiry, WS AF Prabhu, Varun Vijay Hong, Bo Allen, Joshua E. Dicker, David T. Navaraj, Arunasalam Kopelovich, Levy El-Deiry, Wafik S. TI Anti-tumor effects of p53-pathway restoring compound Prodigiosin in colorectal cancer involve effects on apoptotic signaling, angiogenesis and cancer stem cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Prabhu, Varun Vijay; Hong, Bo; Allen, Joshua E.; Dicker, David T.; Navaraj, Arunasalam; El-Deiry, Wafik S.] Penn State Coll Med, Penn State Hershey Canc Inst, Hematol Oncol, Hershey, PA USA. [Kopelovich, Levy] NCI, Div Canc Prevent, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1170 DI 10.1158/1538-7445.AM2012-1170 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503134 ER PT J AU Pressler, HM Sissung, TM Price, DK Figg, WD AF Pressler, Heather M. Sissung, Tristan M. Price, Douglas K. Figg, William D. TI Expression of an androgen transporter, organic anion transporting polypeptide 163, impacts progression and treatment of prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Pressler, Heather M.; Sissung, Tristan M.; Price, Douglas K.; Figg, William D.] NCI, Bethesda, MD 20892 USA. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1848 DI 10.1158/1538-7445.AM2012-1848 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501287 ER PT J AU Price, DK Reece, KM Troutman, SM Pressler, HM Pisle, ST Figg, WD AF Price, Douglas K. Reece, Kelie M. Troutman, Sarah M. Pressler, Heather M. Pisle, Stephen T. Figg, William D. TI Molecular interaction of HIF-1 alpha and the androgen receptor in prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Price, Douglas K.; Reece, Kelie M.; Troutman, Sarah M.; Pressler, Heather M.; Figg, William D.] NCI, Bethesda, MD 20892 USA. [Pisle, Stephen T.] NCI, SAIC Frederick, Frederick, MD 21701 USA. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2206 DI 10.1158/1538-7445.AM2012-2206 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606237 ER PT J AU Prickett, TD Wei, XM Cardenas-Navia, I Teer, J Lin, JC Walia, V Gartner, J Jiang, J Cherukuri, P Molinolo, A Davies, MA Stamke-Hale, K Margulies, EH Rosenberg, SA Samuels, Y AF Prickett, Todd D. Wei, Xiaomu Cardenas-Navia, Isabel Teer, Jamie Lin, Jimmy C. Walia, Vijay Gartner, Jared Jiang, Jiji Cherukuri, Praveen Molinolo, Alfredo Davies, Michael A. Stamke-Hale, Katherine Margulies, Elliott H. Rosenberg, Steven A. Samuels, Yardena TI Exon capture analysis of G-protein coupled receptors reveals activating mutations in GRM3 in melanoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Prickett, Todd D.; Wei, Xiaomu; Cardenas-Navia, Isabel; Teer, Jamie; Walia, Vijay; Gartner, Jared; Jiang, Jiji; Cherukuri, Praveen; Margulies, Elliott H.; Samuels, Yardena] NHGRI, NIH, Bethesda, MD 20892 USA. [Lin, Jimmy C.] Johns Hopkins Univ, Baltimore, MD USA. [Molinolo, Alfredo] NIDCR, NIH, Bethesda, MD USA. [Davies, Michael A.; Stamke-Hale, Katherine] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Rosenberg, Steven A.] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4860 DI 10.1158/1538-7445.AM2012-4860 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501149 ER PT J AU Purdue, MP Moore, L Merino, MJ Boffetta, P Colt, JS Schwartz, KL Bencko, V Davis, FG Graubard, B Janout, V Ruterbusch, JJ Beebe-Dimmer, J Cote, M Shuch, B Mates, D Hofmann, JN Foretova, L Rothman, N Szeszenia-Dabrowska, N Matveev, V Wacholder, S Zaridze, D Linehan, WM Brennan, P Chow, WH AF Purdue, Mark P. Moore, Lee Merino, Maria J. Boffetta, Paolo Colt, Joanne S. Schwartz, Kendra L. Bencko, Vladimir Davis, Faith G. Graubard, Barry Janout, Vladimir Ruterbusch, Julie J. Beebe-Dimmer, Jennifer Cote, Michele Shuch, Brian Mates, Dana Hofmann, Jonathon N. Foretova, Lenka Rothman, Nathaniel Szeszenia-Dabrowska, Neonilia Matveev, Vsevolod Wacholder, Sholom Zaridze, David Linehan, W. Marston Brennan, Paul Chow, Wong-Ho TI An investigation of risk factors for renal cell carcinoma by histologic subtype in two case-control studies SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Purdue, Mark P.; Moore, Lee; Colt, Joanne S.; Graubard, Barry; Hofmann, Jonathon N.; Rothman, Nathaniel; Wacholder, Sholom; Chow, Wong-Ho] NCI, Rockville, MD USA. [Merino, Maria J.; Shuch, Brian; Linehan, W. Marston] NCI, Bethesda, MD 20892 USA. [Boffetta, Paolo] Mt Sinai Sch Med, New York, NY USA. [Schwartz, Kendra L.; Ruterbusch, Julie J.; Beebe-Dimmer, Jennifer; Cote, Michele] Wayne State Univ, Detroit, MI USA. [Bencko, Vladimir] Charles Univ Prague, Prague, Czech Republic. [Davis, Faith G.] Univ Illinois, Chicago, IL USA. [Janout, Vladimir] Palacky Univ, CR-77147 Olomouc, Czech Republic. [Mates, Dana] Inst Publ Hlth, Bucharest, Romania. [Foretova, Lenka] Masaryk Mem Canc Inst, Brno, Czech Republic. [Szeszenia-Dabrowska, Neonilia] Inst Occupat Med, Lodz, Poland. [Matveev, Vsevolod; Zaridze, David] Russian Acad Med Sci, Canc Res Ctr, Moscow, Russia. [Brennan, Paul] Int Agcy Res Canc, F-69372 Lyon, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5515 DI 10.1158/1538-7445.AM2012-5515 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602177 ER PT J AU Raggi, C Marquardt, JU Seo, D Andersen, JB Factor, VM Thorgeirsson, SS AF Raggi, Chiara Marquardt, Jens U. Seo, Daekwan Andersen, Jesper B. Factor, Valentina M. Thorgeirsson, Snorri S. TI Epigenetic reprogramming affects malignant properties of human liver cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Raggi, Chiara; Marquardt, Jens U.; Seo, Daekwan; Andersen, Jesper B.; Factor, Valentina M.; Thorgeirsson, Snorri S.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4261 DI 10.1158/1538-7445.AM2012-4261 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505232 ER PT J AU Ramaswamy, B Lustberg, MB Wesolowski, R Layman, RM Mrozek, E Olson, EM Andreopoulou, E Garcia-Villa, A Chalmers, J Cotrill, JA Nutter, J Ledin, J Schaaf, LJ Bajestani, S Zhao, WQ Geyer, S Chen, A Shapiro, CL Villalona-Calero, MA Knopp, M Grever, MR AF Ramaswamy, Bhuvaneswari Lustberg, Maryam B. Wesolowski, Robert Layman, Rachel M. Mrozek, Ewa Olson, Erin M. Andreopoulou, Eleni Garcia-Villa, Alejandra Chalmers, Jeff Cotrill, Jeffrey A. Nutter, Julie Ledin, Jamie Schaaf, Larry J. Bajestani, Saeed Zhao, Weiqiang Geyer, Susan Chen, Alice Shapiro, Charles L. Villalona-Calero, Miguel A. Knopp, Michael Grever, Michael R. TI Phase I study of PARP inhibitor ABT-888 and carboplatin with novel imaging in metastatic breast cancer (MBC) (NCI-8609) SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ramaswamy, Bhuvaneswari; Lustberg, Maryam B.; Wesolowski, Robert; Layman, Rachel M.; Mrozek, Ewa; Olson, Erin M.; Nutter, Julie; Ledin, Jamie; Shapiro, Charles L.] Ohio State Univ, Stefanie Spielman Comprehens Breast Ctr, Columbus, OH 43210 USA. [Andreopoulou, Eleni] Montefiore Med Ctr, Albert Einstein Coll Med, Bronx, NY 10467 USA. [Garcia-Villa, Alejandra; Chalmers, Jeff; Cotrill, Jeffrey A.; Schaaf, Larry J.; Bajestani, Saeed; Zhao, Weiqiang; Geyer, Susan; Villalona-Calero, Miguel A.; Knopp, Michael; Grever, Michael R.] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA. [Chen, Alice] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5586 DI 10.1158/1538-7445.AM2012-5586 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601166 ER PT J AU Rand, KA Conti, DV Haiman, CA Van Den Berg, DJ Birmann, BM De Roos, AJ Severson, RK Gebregziabher, M Ailawadhi, S Morbacher, A Lieber, MR Wang, SS Bernstein, L Edlund, CK Rothman, N Chanock, SJ Kolonel, LN Colditz, GA Munshi, N Anderson, KC Cozen, W AF Rand, Kristin A. Conti, David V. Haiman, Christopher A. Van Den Berg, David J. Birmann, Brenda M. De Roos, Anneclaire J. Severson, Richard K. Gebregziabher, Mulugeta Ailawadhi, Sikander Morbacher, Ann Lieber, Michael R. Wang, Sophia S. Bernstein, Leslie Edlund, Christopher K. Rothman, Nathaniel Chanock, Stephen J. Kolonel, Laurence N. Colditz, Graham A. Munshi, Nikhil Anderson, Kenneth C. Cozen, Wendy TI Polymorphisms in DNA repair genes and risk of multiple myeloma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Rand, Kristin A.; Conti, David V.; Haiman, Christopher A.; Lieber, Michael R.] Univ So Calif, Keck Sch Med, Dept Prevent Med, Norris Comprehens Canc Ctr, Los Angeles, CA 90033 USA. [Van Den Berg, David J.; Cozen, Wendy] Univ So Calif, Dept Prevent Med, Ctr Comprehens Canc, Los Angeles, CA 90089 USA. [Van Den Berg, David J.; Cozen, Wendy] Univ So Calif, Dept Pathol, Ctr Comprehens Canc, Los Angeles, CA 90089 USA. [Birmann, Brenda M.] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA USA. [Birmann, Brenda M.] Harvard Univ, Sch Med, Boston, MA USA. [De Roos, Anneclaire J.] Fred Hutchinson Canc Res Ctr, Program Epidemiol, Seattle, WA 98104 USA. [Severson, Richard K.] Wayne State Univ, Dept Family Med & Publ Hlth Sci, Detroit, MI USA. [Gebregziabher, Mulugeta] Med Univ S Carolina, Div Biostat & Epidemiol, Charleston, SC 29425 USA. [Ailawadhi, Sikander; Morbacher, Ann] Univ So Calif, Keck Sch Med, Norris Comprehens Canc Ctr, Div Hematol,Dept Med, Los Angeles, CA 90033 USA. [Wang, Sophia S.; Bernstein, Leslie] City Hope Natl Med Ctr, Dept Populat Sci, Div Canc Etiol, Duarte, CA 91010 USA. [Edlund, Christopher K.] Univ So Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90033 USA. [Rothman, Nathaniel; Chanock, Stephen J.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Kolonel, Laurence N.] Univ Hawaii, Canc Res Ctr, Honolulu, HI 96813 USA. [Colditz, Graham A.] Washington Univ, Sch Med, Dept Surg, St Louis, MO 63110 USA. [Colditz, Graham A.] Washington Univ, Sch Med, Alvin J Siteman Canc Ctr, St Louis, MO USA. [Colditz, Graham A.] Barnes Jewish Hosp, St Louis, MO 63110 USA. [Munshi, Nikhil; Anderson, Kenneth C.] Dana Farber Canc Inst, Dept Med Oncol, Jeroma Lipper Multiple Myeloma Ctr, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2634 DI 10.1158/1538-7445.AM2012-2634 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602262 ER PT J AU Ranuncolo, SM Xiao, WM Wright, G Abu-Asab, M Pittaluga, S Jaffe, ES Lewis, B AF Ranuncolo, Stella M. Xiao, Wenming Wright, George Abu-Asab, Mones Pittaluga, Stefania Jaffe, Elaine S. Lewis, Brian TI Hodgkin Lymphoma requires stabilized NIK and constitutive ReIB expression for survival SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ranuncolo, Stella M.; Xiao, Wenming; Wright, George; Abu-Asab, Mones; Pittaluga, Stefania; Jaffe, Elaine S.; Lewis, Brian] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-281 DI 10.1158/1538-7445.AM2012-LB-281 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501267 ER PT J AU Reinhold, WC Sunshine, M Varma, S Liu, HF Weinstein, JN Morris, J Doroshow, J Pommier, Y AF Reinhold, William C. Sunshine, Margot Varma, Sudir Liu, Hongfang Weinstein, John N. Morris, Joel Doroshow, James Pommier, Yves TI Web-based access for whole genome analyses of gene expression, DNA copy number, microRNA transcript levels, drug activity, and their pattern comparisons for the NCI-60 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Reinhold, William C.; Sunshine, Margot; Varma, Sudir; Pommier, Yves] NCI, Bethesda, MD 20892 USA. [Liu, Hongfang] Mayo Clin, Rochester, MN USA. [Weinstein, John N.] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Morris, Joel] Drug Synth & Chem Branch, Rockville, MD USA. [Doroshow, James] NCI, Off Director, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2991 DI 10.1158/1538-7445.AM2012-2991 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604197 ER PT J AU Richardson, ED Reece, KM Campbell, TJ Pressler, H Pisle, ST Figg, WD AF Richardson, Emily D. Reece, Kelie M. Campbell, Tessa J. Pressler, Heather Pisle, Stephen T. Figg, William D. TI Select epidithiodiketopiperazine (ETP) compounds disrupt the HIF-1 alpha/p300 interaction and HIF-mediated transcription SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Richardson, Emily D.; Reece, Kelie M.; Campbell, Tessa J.; Pressler, Heather] NCI, Mol Pharmacol Sect, Med Oncol Branch, Bethesda, MD 20892 USA. [Pisle, Stephen T.] NCI, Clin Pharmacol Program, SAIC Frederick, Frederick, MD 21701 USA. [Figg, William D.] NCI, Clin Pharmacol Program, Med Oncol Branch, Bethesda, MD 20892 USA. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2331 DI 10.1158/1538-7445.AM2012-2331 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504281 ER PT J AU Rivers, RC Mesri, M Kinsinger, C Boja, E Hiltke, T Rodriguez, H AF Rivers, Robert C. Mesri, Mehdi Kinsinger, Chris Boja, Emily Hiltke, Tara Rodriguez, Henry TI NCI's Clinical Proteomic Tumor Analysis Consortium SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Rivers, Robert C.; Mesri, Mehdi; Kinsinger, Chris; Boja, Emily; Hiltke, Tara; Rodriguez, Henry] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1282 DI 10.1158/1538-7445.AM2012-1282 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502385 ER PT J AU Roberts, NJ Jiao, YC Yu, J Kopelovich, L Petersen, GM Bondy, M Gallinger, S Schwartz, AG Syngai, S Cote, ML Axilbund, J Schulick, R Ali, SZ Eshleman, JR Velculescu, V Goggins, M Vogelstein, B Papadopoulous, N Hruban, RH Kinzler, KW Klein, AP AF Roberts, Nicholas J. Jiao, Yuchen Yu, Jun Kopelovich, Levy Petersen, Gloria M. Bondy, Melissa Gallinger, Steven Schwartz, Ann G. Syngai, Sapna Cote, Michele L. Axilbund, Jennifer Schulick, Richard Ali, Syed Z. Eshleman, James R. Velculescu, Victor Goggins, Michael Vogelstein, Bert Papadopoulous, Nikolas Hruban, Ralph H. Kinzler, Kenneth W. Klein, Alison P. TI Genome-wide sequencing identifies ATM as a pancreatic cancer susceptibility gene SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Roberts, Nicholas J.; Jiao, Yuchen; Yu, Jun; Axilbund, Jennifer; Schulick, Richard; Ali, Syed Z.; Eshleman, James R.; Velculescu, Victor; Goggins, Michael; Vogelstein, Bert; Papadopoulous, Nikolas; Hruban, Ralph H.; Kinzler, Kenneth W.; Klein, Alison P.] Johns Hopkins Univ, Baltimore, MD USA. [Kopelovich, Levy] NCI, Bethesda, MD 20892 USA. [Petersen, Gloria M.] Mayo Clin, Rochester, MN USA. [Bondy, Melissa] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Gallinger, Steven] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada. [Schwartz, Ann G.; Cote, Michele L.] Wayne State Univ, Karmanos Canc Inst, Detroit, MI USA. [Syngai, Sapna] Dana Farber Canc Inst, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2628 DI 10.1158/1538-7445.AM2012-2628 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602255 ER PT J AU Robey, RW Luchenko, VL Bahr, J Chakraborty, AR Zimmer, AS Piekarz, RL Bates, SE AF Robey, Robert W. Luchenko, Victoria L. Bahr, Julian Chakraborty, Arup R. Zimmer, Alexandra S. Piekarz, Richard L. Bates, Susan E. TI Combined mitogen-activated protein kinase pathway inhibition with short-term romidepsin treatment induces proapoptotic Bim and cell death in BRAF mutant cancers SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Robey, Robert W.; Luchenko, Victoria L.; Bahr, Julian; Chakraborty, Arup R.; Piekarz, Richard L.; Bates, Susan E.] NCI, CCR, Bethesda, MD 20892 USA. [Zimmer, Alexandra S.] Washington Hosp Ctr, Washington, DC 20010 USA. RI Zimmer, Alexandra/K-6824-2016 NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4709 DI 10.1158/1538-7445.AM2012-4709 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603339 ER PT J AU Rodina, A Moulick, K Ahn, J Zong, HL Cerchietti, L DaGama, EG Caldas-Lopes, E Beebe, K Perna, F Hatzi, K Vu, L Zhao, XY Zatorska, D Taldone, T Smith-Jones, P Alpaugh, M Gross, S Pillarsetty, N Ku, T Lewis, J Larson, S Levine, R Erdjument-Bromage, H Guzman, M Nimer, S Melnick, A Neckers, L Chiosis, G AF Rodina, Anna Moulick, Kamalika Ahn, James Zong, Hongliang Cerchietti, Leandro DaGama, Erica Gomes Caldas-Lopes, Eloisi Beebe, Kristin Perna, Fabiana Hatzi, Katerina Vu, Ly Zhao, Xinyang Zatorska, Danuta Taldone, Tony Smith-Jones, Peter Alpaugh, Mary Gross, Steven Pillarsetty, Nagavarakishore Ku, Thomas Lewis, Jason Larson, Steven Levine, Ross Erdjument-Bromage, Hediye Guzman, Monica Nimer, Stephen Melnick, Ari Neckers, Len Chiosis, Gabriela TI Biochemical evidence towards the existence of an oncogenic Hsp90 complex SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Rodina, Anna; Moulick, Kamalika; Ahn, James; DaGama, Erica Gomes; Caldas-Lopes, Eloisi; Perna, Fabiana; Vu, Ly; Zhao, Xinyang; Zatorska, Danuta; Taldone, Tony; Smith-Jones, Peter; Alpaugh, Mary; Pillarsetty, Nagavarakishore; Ku, Thomas; Lewis, Jason; Larson, Steven; Levine, Ross; Erdjument-Bromage, Hediye; Nimer, Stephen; Chiosis, Gabriela] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. [Zong, Hongliang; Cerchietti, Leandro; Hatzi, Katerina; Gross, Steven; Guzman, Monica; Melnick, Ari] Weill Cornell Med Coll, New York, NY USA. [Beebe, Kristin; Neckers, Len] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3029 DI 10.1158/1538-7445.AM2012-3029 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604239 ER PT J AU Ruczinski, I Jorgensen, T Shugart, YY Berthier-Schaad, Y Kessing, B Hoffman-Bolton, J Helzlsouer, K Kao, WHL Wheless, L Francis, L Alani, R Strickland, P Smith, M Alberg, AJ AF Ruczinski, Ingo Jorgensen, Timothy Shugart, Yin Yao Berthier-Schaad, Yvette Kessing, Bailey Hoffman-Bolton, Judithh Helzlsouer, Kathy Kao, W. H. Linda Wheless, Lee Francis, Lesley Alani, Rhoda Strickland, Paul Smith, Michael Alberg, Anthony J. TI Nonmelanoma skin cancer as a marker of a cancer-prone phenotype: Potential role of DNA repair gene variants SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Ruczinski, Ingo; Berthier-Schaad, Yvette; Hoffman-Bolton, Judithh; Kao, W. H. Linda] Johns Hopkins Univ, Baltimore, MD USA. [Jorgensen, Timothy] Georgetown Univ, Washington, DC USA. [Shugart, Yin Yao] NIMH, Rockville, MD 20857 USA. [Kessing, Bailey] NCI, Frederick, MD 21701 USA. [Helzlsouer, Kathy] Mercy Med Ctr, Baltimore, MD USA. [Wheless, Lee; Francis, Lesley; Alberg, Anthony J.] Med Univ S Carolina, Charleston, SC 29425 USA. [Alani, Rhoda] Boston Univ, Sch Med, Boston, MA 02118 USA. [Smith, Michael] SAIC Frederick, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2636 DI 10.1158/1538-7445.AM2012-2636 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602264 ER PT J AU Russell, EA Gembarska, A Marine, JC Bernal, F AF Russell, Elisabeth A. Gembarska, Agnieszka Marine, Jean-Christophe Bernal, Federico TI Inhibition of the p53-HDMX interaction sensitizes melanoma to chemotherapy SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Russell, Elisabeth A.; Bernal, Federico] NCI, Bethesda, MD 20892 USA. [Gembarska, Agnieszka; Marine, Jean-Christophe] KULeuven, Leuven, Belgium. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4730 DI 10.1158/1538-7445.AM2012-4730 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604028 ER PT J AU Saleh, AD Si, H Lu, H Yang, XPY Chen, Z Van Waes, C Coupar, J AF Saleh, Anthony D. Si, Han Lu, Hai Yang, Xinping Yang Chen, Zhong Van Waes, Carter Coupar, Jamie TI Transcriptome sequencing identifies oncogenic microRNA signatures that interact with NF-kappaB, TP53 and Notch signaling in head and neck squamous cell carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Saleh, Anthony D.; Si, Han; Lu, Hai; Yang, Xinping Yang; Chen, Zhong; Van Waes, Carter; Coupar, Jamie] NIDCD, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3177 DI 10.1158/1538-7445.AM2012-3177 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503132 ER PT J AU Saloustros, E Mertz, E Nesterova, M Keil, M Horvath, A Nadella, K Holmbeck, K Liu, SS Mandas, V Shikha, K Leikin, S Robey, P Stratakis, CA AF Saloustros, Emmanouil Mertz, Edward Nesterova, Maria Keil, Meg Horvath, Anelia Nadella, Kiran Holmbeck, Kenn Liu, Sisi Mandas, Vince Shikha, Kapil Leikin, Sergey Robey, Pamela Stratakis, Constantine A. TI COX-2 inhibition reduces bone tumor growth in animal models:A role for celecoxib treatment in cAMP/protein kinase A-induced tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Saloustros, Emmanouil; Nesterova, Maria; Keil, Meg; Horvath, Anelia; Nadella, Kiran; Liu, Sisi; Mandas, Vince; Shikha, Kapil; Stratakis, Constantine A.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Endocrinol & Genet, Program Dev Endocrinol & Genet, NIH, Bethesda, MD USA. [Mertz, Edward; Leikin, Sergey] NICHHD, Sect Phys Biochem, Off Sci Director, NIH, Bethesda, MD 20892 USA. [Holmbeck, Kenn; Robey, Pamela] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 954 DI 10.1158/1538-7445.AM2012-954 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602007 ER PT J AU Saloustros, E Tsang, K Mertz, E Starost, M Nesterova, M Nadella, K Horvath, A Sierra, L Liu, S Cole, ME Holmbeck, K Leikin, S Robey, P Stratakis, C AF Saloustros, Emmanouil Tsang, Kitman Mertz, Edward Starost, Matthew Nesterova, Maria Nadella, Kiran Horvath, Anelia Sierra, Lucy Liu, Sisi Cole, Mary E. Holmbeck, Kenn Leikin, Sergey Robey, Pamela Stratakis, Constantine TI A mouse model of double heterozygosity for protein kinase A regulatory subunits promotes osteoblastic differentiation of cAMP-induced bone tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Saloustros, Emmanouil; Tsang, Kitman; Nesterova, Maria; Nadella, Kiran; Horvath, Anelia; Sierra, Lucy; Liu, Sisi; Stratakis, Constantine] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Endocrinol & Genet, Program Dev Endocrinol & Genet, Bethesda, MD USA. [Mertz, Edward; Leikin, Sergey] NICHHD, Sect Phys Biochem, Off Sci Director, NIH, Bethesda, MD 20892 USA. [Starost, Matthew] NIH, Off Res Serv, Div Vet Resources, Bethesda, MD 20892 USA. [Cole, Mary E.] NICHHD, Sect Phys Biochem, Off Sci Director, NIH, Bethesda, MD 20892 USA. [Holmbeck, Kenn; Robey, Pamela] Natl Inst Dent Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1360 DI 10.1158/1538-7445.AM2012-1360 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504409 ER PT J AU Sato, N Aras, O Choyke, P AF Sato, Noriko Aras, Omer Choyke, Peter TI Near-infrared optical imaging visualizes tumor cell death induced by adoptive transferred T cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Sato, Noriko; Aras, Omer; Choyke, Peter] NCI, Mol Imaging Program, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4291 DI 10.1158/1538-7445.AM2012-4291 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505428 ER PT J AU Schwentner, R Bilke, S Kauer, M Jug, G Walker, RL Meltzer, PS Kovar, H AF Schwentner, Raphaela Bilke, Sven Kauer, Max Jug, Gunhild Walker, Robert L. Meltzer, Paul S. Kovar, Heinrich TI A functional ETS/E2F module in cancers expressing ETS fusion genes SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Schwentner, Raphaela; Kauer, Max; Jug, Gunhild; Kovar, Heinrich] St Anna Childrens Hosp, Childrens Canc Res Inst, A-1090 Vienna, Austria. [Bilke, Sven; Walker, Robert L.; Meltzer, Paul S.] NCI, Genet Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2198 DI 10.1158/1538-7445.AM2012-2198 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606229 ER PT J AU Segars, LE Mendoza, A Helman, LJ Grohar, PJ AF Segars, Laura E. Mendoza, Arnulfo Helman, Lee J. Grohar, Patrick J. TI ET-743 interferes with EWS-FLI1 driven WRN expression in Ewing sarcoma cells and sensitizes to topoisomerase I inhibitors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Segars, Laura E.; Mendoza, Arnulfo; Helman, Lee J.] NCI, Bethesda, MD 20892 USA. [Grohar, Patrick J.] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2497 DI 10.1158/1538-7445.AM2011-2497 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501493 ER PT J AU Setiawan, VW Yang, HP Pike, MC McCann, S Yu, H Xiang, YB Wolle, A Wentzensen, N Weiss, NS Webb, PM van den Brande, PA van de Vijver, K Thompson, PJ Strom, BL Spurdle, AB Shu, XO Schairer, C Sacerdote, C Rohan, TE Robien, K Risch, H Ricceri, F Rebbeck, TR Rastogi, R Prescott, J Polidoro, S Park, Y Olson, SH Moysich, KB Miller, AB McCullough, ML Matsuno, RK Magliocco, AM Lurie, G Lu, LG Lissowska, J Liang, XL Lacey, JV Kolonel, LN Henderson, BE Hankinson, SE Hakansson, N Goodman, MT Gaudet, MM Garcia-Closas, M Friedenreich, C Freudenheim, JL Doherty, J AF Setiawan, Veronica Wendy Yang, Hannah P. Pike, Malcolm C. McCann, Susan Yu, Herbert Xiang, Yong-Bing Wolle, Alicia Wentzensen, Nicolas Weiss, Noel S. Webb, Penelope M. van den Brande, Piet A. van de Vijver, Koen Thompson, Pamela J. Strom, Brian L. Spurdle, Amanda B. Shu, Xiao-ou Schairer, Catherine Sacerdote, Carlotta Rohan, Thomas E. Robien, Kim Risch, Harvey Ricceri, Fulvio Rebbeck, Timothy R. Rastogi, Radhai Prescott, Jennifer Polidoro, Silvia Park, Yikyung Olson, Sara H. Moysich, Kirsten B. Miller, Anthony B. McCullough, Marjorie L. Matsuno, Rayna K. Magliocco, Anthony M. Lurie, Galina Lu, Lingeng Lissowska, Jolanta Liang, Xiaolin Lacey, James V. Kolonel, Laurence N. Henderson, Brian E. Hankinson, Susan E. Hakansson, Niclas Goodman, Marc T. Gaudet, Mia M. Garcia-Closas, Montserrat Friedenreich, Christine Freudenheim, Jo L. Doherty, Jennifer CA Australian Natl Endometrial Canc TI The association of body mass index with risk of endometrial cancer subtypes: Pooled analysis in E2C2 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Setiawan, Veronica Wendy; Pike, Malcolm C.; Henderson, Brian E.] Univ So Calif, Los Angeles, CA USA. [Yang, Hannah P.; Wentzensen, Nicolas; Schairer, Catherine; Park, Yikyung] NCI, Bethesda, MD 20892 USA. [McCann, Susan; Moysich, Kirsten B.] Roswell Pk Canc Inst, Buffalo, NY 14263 USA. [Yu, Herbert; Risch, Harvey; Lu, Lingeng] Yale Univ, New Haven, CT USA. [Xiang, Yong-Bing] Shanghai Canc Inst, Shanghai, Peoples R China. [Wolle, Alicia; Hakansson, Niclas] Karolinska Inst, Stockholm, Sweden. [Weiss, Noel S.] Univ Washington, Seattle, WA 98195 USA. [Webb, Penelope M.; Spurdle, Amanda B.] Queensland Inst Med Res, Brisbane, Qld 4006, Australia. [van den Brande, Piet A.; van de Vijver, Koen] Maastricht Univ, NL-6200 MD Maastricht, Netherlands. [Thompson, Pamela J.; Matsuno, Rayna K.; Lurie, Galina; Kolonel, Laurence N.; Goodman, Marc T.] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. [Strom, Brian L.; Rebbeck, Timothy R.] Univ Penn, Sch Med, Philadelphia, PA 19104 USA. [Shu, Xiao-ou] Vanderbilt Univ, Med Ctr, Nashville, TN USA. [Sacerdote, Carlotta] CPO Piemonte, Turin, Italy. [Rohan, Thomas E.] Albert Einstein Coll Med, Bronx, NY 10467 USA. [Robien, Kim] Univ Minnesota, Minneapolis, MN USA. [Ricceri, Fulvio; Polidoro, Silvia] Human Genet Fdn HuGeF, Turin, Italy. [Rastogi, Radhai; Olson, Sara H.; Liang, Xiaolin] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. [Prescott, Jennifer] Brigham & Womens Hosp, Boston, MA 02115 USA. [Prescott, Jennifer] Harvard Univ, Sch Med, Boston, MA USA. [Miller, Anthony B.] Univ Toronto, Dalla Lana Sch Publ Hlth, Toronto, ON, Canada. [McCullough, Marjorie L.; Gaudet, Mia M.] Amer Canc Soc, Atlanta, GA 30329 USA. [Magliocco, Anthony M.] Univ S Florida, H Lee Moffitt Canc Ctr, Tampa, FL 33682 USA. [Lissowska, Jolanta] M Sklowdowska Curie Mem Canc Ctr & Inst Oncol, Warsaw, Poland. [Lacey, James V.] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA USA. [Hankinson, Susan E.] Univ Massachusetts, Amherst, MA 01003 USA. [Garcia-Closas, Montserrat] Inst Canc Res, Surrey, England. [Friedenreich, Christine] Alberta Hlth Serv Canc Care, Calgary, AB, Canada. [Freudenheim, Jo L.] Univ Buffalo, SUNY, Buffalo, NY USA. [Doherty, Jennifer] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1029 DI 10.1158/1538-7445.AM2012-1029 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701602400 ER PT J AU Severson, PL Tokar, EJ Vrba, L Waalkes, MP Futscher, BW AF Severson, Paul L. Tokar, Erik J. Vrba, Lukas Waalkes, Michael P. Futscher, Bernard W. TI Common targets of epigenetic dysfunction in distinct target tissues of arsenic and cadmium induced malignant transformation SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Severson, Paul L.; Futscher, Bernard W.] Univ Arizona, Coll Pharm, Tucson, AZ 85721 USA. [Tokar, Erik J.; Waalkes, Michael P.] NIEHS, Natl Toxicol Program Lab, Res Triangle Pk, NC 27709 USA. [Vrba, Lukas] Univ Arizona, Arizona Canc Ctr, Tucson, AZ USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5448 DI 10.1158/1538-7445.AM2012-5448 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600195 ER PT J AU Shi, JX Li, P AF Shi, Jianxin Li, Peng TI A novel statistical method for detecting association of copy number variations in genome-wide association studies of cancers SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Shi, Jianxin; Li, Peng] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3969 DI 10.1158/1538-7445.AM2012-3969 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605071 ER PT J AU Shi, JX Chatterjee, N Rotunno, M Wang, YF Pesatori, AC Consonni, D Li, P Broderick, P Henrion, M Eisen, T Wang, ZM Chen, W Dong, Q Albanes, D Thun, M Spitz, M Bertazzi, PA Caporaso, N Chanock, S Amos, C Houlston, R Landi, MT AF Shi, Jianxin Chatterjee, Nilanjan Rotunno, Melissa Wang, Yufei Pesatori, Angela C. Consonni, Dario Li, Peng Broderick, Peter Henrion, Marc Eisen, Timothy Wang, Zhaoming Chen, Wei Dong, Qiong Albanes, Demetrius Thun, Michael Spitz, Margaret Bertazzi, Pier Alberto Caporaso, Neil Chanock, Stephen Amos, Christopher Houlston, Richard Landi, Maria Teresa TI Inherited variation at chromosome 12p13.33 including RAD52 influences squamous cell lung carcinoma risk SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Shi, Jianxin; Chatterjee, Nilanjan; Rotunno, Melissa; Pesatori, Angela C.; Consonni, Dario; Li, Peng; Wang, Zhaoming; Albanes, Demetrius; Bertazzi, Pier Alberto; Caporaso, Neil; Chanock, Stephen; Landi, Maria Teresa] NCI, DCEG, Bethesda, MD 20892 USA. [Wang, Yufei; Broderick, Peter; Henrion, Marc; Houlston, Richard] ICR, Sutton, Surrey, England. [Eisen, Timothy] Univ Cambridge, Cambridge, England. [Chen, Wei; Dong, Qiong; Spitz, Margaret; Amos, Christopher] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Thun, Michael] Amer Canc Soc, Atlanta, GA 30329 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2931 DI 10.1158/1538-7445.AM2012-2931 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603024 ER PT J AU Shukla, S Shankavaram, U Smart, D AF Shukla, Sudhanshu Shankavaram, Uma Smart, DeeDee TI Proteomic analysis of acute radiation response following whole brain irradiation: Is Sirt2 a key player of radiation induced neurotoxicity SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Shukla, Sudhanshu; Shankavaram, Uma; Smart, DeeDee] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4354 DI 10.1158/1538-7445.AM2012-4354 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502054 ER PT J AU Si, H Lu, H Yang, XP Jang, MY Burnett, J Davis, S Sun, HW Xiao, WM Wei, L Meltzer, P Van Waes, C Chen, Z AF Si, Han Lu, Hai Yang, Xinping Jang, Minyoung Burnett, Jeffery Davis, Sean Sun, Hong-wei Xiao, Wenming Wei, Lai Meltzer, Paul Van Waes, Carter Chen, Zhong TI TNF-alpha dynamically modulates genome-wide cross-regulation of cRel, Delta Np63 and TAp73 promoter binding and gene expression in head and neck cancer: TP53 and NF kappa B ChIP-Seq in HNSCC SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Si, Han; Lu, Hai; Yang, Xinping; Jang, Minyoung; Burnett, Jeffery; Van Waes, Carter; Chen, Zhong] NIDCD, NIH, Bethesda, MD USA. [Davis, Sean; Meltzer, Paul] NCI, NIH, Bethesda, MD 20892 USA. [Sun, Hong-wei] NIAMS, NIH, Bethesda, MD USA. [Xiao, Wenming] NIH, CIT, Bethesda, MD 20892 USA. [Wei, Lai] NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4193 DI 10.1158/1538-7445.AM2012-4193 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502430 ER PT J AU Smith, M Keir, S Maris, J Kolb, A Reynolds, P Kang, M Carol, H Lock, R Gorlick, R Kurmasheva, R Billups, C Houghton, P AF Smith, Malcolm Keir, Stephen Maris, John Kolb, Anders Reynolds, Patrick Kang, Min Carol, Hernan Lock, Richard Gorlick, Richard Kurmasheva, Raushan Billups, Catherine Houghton, Peter TI Pediatric Preclinical Testing Program (PPTP) evaluation of volasertib (BI 6727), a Polo-like kinase (PLK) inhibitor SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Smith, Malcolm] NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA. [Keir, Stephen] Duke Univ, Med Ctr, Durham, NC USA. [Maris, John] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Kolb, Anders] Alfred I DuPont Hosp Children, Wilmington, DE USA. [Reynolds, Patrick; Kang, Min] Texas Tech Univ, Hlth Sci Ctr, Lubbock, TX 79430 USA. [Carol, Hernan; Lock, Richard] Childrens Canc Inst Australia, Randwick, NSW, Australia. [Gorlick, Richard] Montefiore Med Ctr, Bronx, NY 10467 USA. [Kurmasheva, Raushan; Houghton, Peter] Nationwide Childrens Hosp, Columbus, OH USA. [Billups, Catherine] St Jude Childrens Res Hosp, Memphis, TN 38105 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-317 DI 10.1158/1538-7445.AM2012-LB-317 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504220 ER PT J AU Solomon, D Kim, T Diaz-Martinez, L Fair, J Elkahloun, A Harris, B Toretsky, J Rosenberg, S Shukla, N Ladanyi, M Samuels, Y James, CD Yu, HT Kim, JS Waldman, T AF Solomon, David Kim, Taeyon Diaz-Martinez, Laura Fair, Joshlean Elkahloun, Abdel Harris, Brent Toretsky, Jeffrey Rosenberg, Steven Shukla, Neerav Ladanyi, Marc Samuels, Yardena James, C. David Yu, Hongtao Kim, Jung-Sik Waldman, Todd TI Mutational inactivation of STAG2 causes aneuploidy in human cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Solomon, David; Kim, Taeyon; Fair, Joshlean; Harris, Brent; Toretsky, Jeffrey; Kim, Jung-Sik; Waldman, Todd] Georgetown Univ, Sch Med, Washington, DC USA. [Diaz-Martinez, Laura; Yu, Hongtao] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA. [Elkahloun, Abdel; Samuels, Yardena] NHGRI, NIH, Bethesda, MD 20892 USA. [Rosenberg, Steven] NCI, NIH, Bethesda, MD 20892 USA. [Shukla, Neerav; Ladanyi, Marc] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. [James, C. David] UCSF, San Francisco, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3114 DI 10.1158/1538-7445.AM2012-3114 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605202 ER PT J AU Spitzner, M Roesler, B Bielfeld, C Gaedcke, J Rave-Frank, M Beissbarth, T Ried, T Ghadimi, BM Grade, M AF Spitzner, Melanie Roesler, Birte Bielfeld, Christian Gaedcke, Jochen Rave-Fraenk, Margret Beissbarth, Tim Ried, Thomas Ghadimi, B. Michael Grade, Marian TI Stat3 is a potential molecular target for chemoradiosensitization of colorectal cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Spitzner, Melanie; Roesler, Birte; Bielfeld, Christian; Gaedcke, Jochen; Rave-Fraenk, Margret; Beissbarth, Tim; Ghadimi, B. Michael; Grade, Marian] Univ Med Ctr Gottingen, Gottingen, Germany. [Ried, Thomas] NCI, Genet Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3446 DI 10.1158/1538-7445.AM2012-3446 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502014 ER PT J AU Srivastava, AK Hollingshead, MH Weiner, J Park, SR Kinders, RJ Kummar, S Tomaszewski, J Parchment, RE Doroshow, JH AF Srivastava, Apurva K. Hollingshead, Melinda H. Weiner, Jennifer Park, Sook Ryun Kinders, Robert J. Kummar, Shivaani Tomaszewski, Joseph Parchment, Ralph E. Doroshow, James H. TI Preclinical assessment of MET modulation by a VEGFR inhibitor/MET inhibitor combination that shows additive antitumor efficacy SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Srivastava, Apurva K.; Weiner, Jennifer; Kinders, Robert J.; Parchment, Ralph E.] SAIC Frederick, Frederick, MD USA. [Hollingshead, Melinda H.] NCI, Frederick, MD 21701 USA. [Park, Sook Ryun; Kummar, Shivaani; Tomaszewski, Joseph; Doroshow, James H.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3672 DI 10.1158/1538-7445.AM2012-3672 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601231 ER PT J AU Takata, Y Shu, XO Yang, G Li, HL Dai, Q Gao, J Cai, QY Chow, WH Gao, YT Zheng, W AF Takata, Yumie Shu, Xiao Ou Yang, Gong Li, Honglan Dai, Qi Gao, Jing Cai, Qiuyin Chow, Wong-Ho Gao, Yutang Zheng, Wei TI Association between dietary calcium intake and lung cancer risk in female non-smokers: A report from the Shanghai Women's Health Study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Takata, Yumie; Shu, Xiao Ou; Yang, Gong; Dai, Qi; Cai, Qiuyin; Zheng, Wei] Vanderbilt Univ, Med Ctr, Nashville, TN USA. [Li, Honglan; Gao, Jing; Gao, Yutang] Shanghai Canc Inst, Shanghai, Peoples R China. [Chow, Wong-Ho] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 631 DI 10.1158/1538-7445.AM2012-631 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602086 ER PT J AU Tanaka, N Jeong, JH Gavin, PG Pogue-Geile, KL Paik, S Costantino, JP AF Tanaka, Noriko Jeong, Jong-Hyeon Gavin, Patrick G. Pogue-Geile, Katherine L. Paik, Soonmyung Costantino, Joseph P. TI Graphical method to predict drug response using genomic biomarkers with nonlinear interaction effect SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Tanaka, Noriko; Jeong, Jong-Hyeon; Gavin, Patrick G.; Pogue-Geile, Katherine L.; Paik, Soonmyung; Costantino, Joseph P.] NSABP, Pittsburgh, PA USA. [Tanaka, Noriko; Jeong, Jong-Hyeon; Costantino, Joseph P.] NSABP Biostat Ctr, Pittsburgh, PA USA. [Tanaka, Noriko; Jeong, Jong-Hyeon; Costantino, Joseph P.] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Biostat, Pittsburgh, PA 15261 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2711 DI 10.1158/1538-7445.AM2012-2711 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701601349 ER PT J AU Tang, W Jones, TL Pitt, J Ambs, S Caplen, NJ Prokunina, L AF Tang, Wei Jones, Tamara L. Pitt, Jason Ambs, Stefan Caplen, Natasha J. Prokunina, Ludmila TI From genome-wide association studies (GWAS) to functional genomics of prostate cancer: exploring the role of candidate transcripts through RNAi-based analysis SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Tang, Wei; Prokunina, Ludmila] NCI, DCEG, Gaithersburg, MD USA. [Jones, Tamara L.; Caplen, Natasha J.] NCI, CCR, CGB, Bethesda, MD 20892 USA. [Pitt, Jason] Univ Chicago, Chicago, IL 60637 USA. [Ambs, Stefan] NCI, CCR, LHC, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 96 DI 10.1158/1538-7445.AM2012-96 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605371 ER PT J AU Tang, W Fu, YP Figueroa, JD Malats, N Garcia-Closas, M Chatterjee, N Kogevinas, M Chanock, SJ Silverman, DT Rothman, N Prokunina-Olsson, L AF Tang, Wei Fu, Yi-Ping Figueroa, Jonine D. Malats, Nuria Garcia-Closas, Montserrat Chatterjee, Nilanjan Kogevinas, Manolis Chanock, Stephen J. Silverman, Debra T. Rothman, Nathaniel Prokunina-Olsson, Ludmila TI Post-GWAS genetic and functional studies for bladder cancer: An uncommon protective synonymous coding variant within the UGT1A6 gene explains the GWAS signal and affects cellular detoxification SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Tang, Wei; Fu, Yi-Ping; Prokunina-Olsson, Ludmila] NCI, DCEG, Lab Translat Genom, Bethesda, MD 20892 USA. [Figueroa, Jonine D.; Chatterjee, Nilanjan; Chanock, Stephen J.; Silverman, Debra T.; Rothman, Nathaniel] NCI, DCEG, Bethesda, MD 20892 USA. [Malats, Nuria] Spanish Natl Canc Res Ctr, Madrid, Spain. [Garcia-Closas, Montserrat] Inst Canc Res, Div Genet & Epidemiol, London SW3 6JB, England. [Kogevinas, Manolis] Ctr Res Environm Epidemiol CREAL, Barcelona, Spain. RI Kogevinas, Manolis/C-3918-2017 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1658 DI 10.1158/1538-7445.AM2012-1658 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602205 ER PT J AU Tao, MH Marian, C Shields, P Postischman, N Nie, J Ambrosone, C Edge, S Krishnan, S Vito, D Trevisan, M Freudenheim, J AF Tao, Menghua Marian, Catalin Shields, Peter Postischman, Nancy Nie, Jing Ambrosone, Christine Edge, Stephen Krishnan, Shiva Vito, Dominica Trevisan, Maurizio Freudenheim, Jo TI Early life exposures and promoter methylation in breast cancer: the Western New York Exposures and Breast Cancer (WEB) Study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Tao, Menghua] Mt Sinai Sch Med, Inst Translat Epidemiol, New York, NY USA. [Marian, Catalin; Shields, Peter; Krishnan, Shiva] Ohio State Univ, Ctr Canc, Columbus, OH 43210 USA. [Postischman, Nancy] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Nie, Jing; Vito, Dominica; Trevisan, Maurizio; Freudenheim, Jo] Univ Buffalo, Dep Social & Prevent Med, Buffalo, NY USA. [Ambrosone, Christine] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Buffalo, NY 14263 USA. [Edge, Stephen] Roswell Pk Canc Inst, Dept Surg, Buffalo, NY 14263 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4483 DI 10.1158/1538-7445.AM2012-4483 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602012 ER PT J AU Thompson, DN Barrett, RA Deonarine, A Williams, CD White-Coleman, D Thomas, A Horton, S Cross, AJ Laiyemo, AO AF Thompson, Dianne N. Barrett, Richard A. Deonarine, Anand Williams, Carla D. White-Coleman, Debra Thomas, Alicia Horton, Sara Cross, Amanda J. Laiyemo, Adeyinka O. TI The effect of lung cancer screening awareness on smoking behavior among US adults SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Thompson, Dianne N.; Barrett, Richard A.] Howard Univ, Coll Med, Washington, DC USA. [Deonarine, Anand; White-Coleman, Debra; Thomas, Alicia; Horton, Sara; Laiyemo, Adeyinka O.] Howard Univ, Coll Med, Dept Med, Washington, DC USA. [Williams, Carla D.] Howard Univ, Ctr Canc, Washington, DC 20059 USA. [Cross, Amanda J.] NCI, Nutr Epidemiol Branch, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 654 DI 10.1158/1538-7445.AM2012-654 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602109 ER PT J AU Trabert, B Erickson, RL Graubard, BI Stanczyk, FZ McGlynn, KA AF Trabert, Britton Erickson, Ralph L. Graubard, Barry I. Stanczyk, Frank Z. McGlynn, Katherine A. TI Pre-diagnostic gonadotropin levels and development of testicular germ cell tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Trabert, Britton; Graubard, Barry I.; McGlynn, Katherine A.] NCI, Div Canc Epidemiol & Genet, Rockville, MD USA. [Erickson, Ralph L.] Walter Reed Army Inst Res, Silver Spring, MD USA. [Stanczyk, Frank Z.] Univ So Calif, Keck Sch Med, Los Angeles, CA 90033 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4470 DI 10.1158/1538-7445.AM2012-4470 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603368 ER PT J AU Trucco, M Wilky, BA Awad, O Shah, P Gul, N Xia, MH Huang, RL Loeb, DM AF Trucco, Matteo Wilky, Breelyn A. Awad, Ola Shah, Preeti Gul, Naheed Xia, Menghang Huang, Ruili Loeb, David M. TI Establishment of a chordoma xenograft and in vivo testing of compounds identified by high-throughput screening SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Trucco, Matteo; Wilky, Breelyn A.; Shah, Preeti; Gul, Naheed; Loeb, David M.] Johns Hopkins Univ, Baltimore, MD USA. [Awad, Ola] Suez Canal Univ, Ismailia, Egypt. [Xia, Menghang; Huang, Ruili] NIH, Chem Genom Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-110 DI 10.1158/1538-7445.AM2012-LB-110 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504187 ER PT J AU Tucker, JA Huen, NY Pang, ALY Lee, TL Vergati, M Intrivici, C Cereda, V Jochems, C Chan, WY Rennert, O Gulley, JL Schlom, J Tsang, KY AF Tucker, Jo A. Huen, Ngar-Yee Pang, Alan L. Y. Lee, Tin-Lap Vergati, Matteo Intrivici, Chiara Cereda, Vittore Jochems, Caroline Chan, Wai-Yee Rennert, Owen Gulley, James L. Schlom, Jeffrey Tsang, Kwong-Yok TI Up-regulation of proliferative and migratory genes in regulatory T cells from patients with metastatic castration-resistant prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Tucker, Jo A.; Huen, Ngar-Yee; Vergati, Matteo; Intrivici, Chiara; Cereda, Vittore; Jochems, Caroline; Gulley, James L.; Schlom, Jeffrey; Tsang, Kwong-Yok] NCI, Bethesda, MD 20892 USA. [Pang, Alan L. Y.; Lee, Tin-Lap; Chan, Wai-Yee; Rennert, Owen] NICHHD, Bethesda, MD 20892 USA. RI Gulley, James/K-4139-2016 OI Gulley, James/0000-0002-6569-2912 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5404 DI 10.1158/1538-7445.AM2012-5404 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500476 ER PT J AU Valdora, F Freier, F Seyler, C Brady, N Kranz, D Hielscher, T Bender, S Northcott, P Kool, M Jones, D Witt, H Ryzhova, M Pleier, S Coco, S Tonini, GP Benner, A von Deimling, A Scheurlen, W Boutros, M Taylor, M Katus, H Kulozik, A Lichter, P Korshunov, A Zitron, E Pfister, S Remke, M AF Valdora, Francesca Freier, Florian Seyler, Claudia Brady, Nathan Kranz, Dominique Hielscher, Thomas Bender, Sebastian Northcott, Paul Kool, Marcel Jones, David Witt, Hendrik Ryzhova, Marina Pleier, Sabrina Coco, Simona Tonini, Gian Paolo Benner, Axel von Deimling, Andreas Scheurlen, Wolfram Boutros, Michael Taylor, Michael Katus, Hugo Kulozik, Andreas Lichter, Peter Korshunov, Andrey Zitron, Edgar Pfister, Stefan Remke, Marc TI KCNJ2 comprises a marker of poor prognosis and a therapeutic target in non-WNT/non-SHH medulloblastoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Valdora, Francesca; Freier, Florian; Brady, Nathan; Kranz, Dominique; Hielscher, Thomas; Bender, Sebastian; Kool, Marcel; Jones, David; Witt, Hendrik; Pleier, Sabrina; Benner, Axel; Boutros, Michael; Lichter, Peter; Pfister, Stefan; Remke, Marc] German Canc Res Ctr, Heidelberg, Germany. [Seyler, Claudia; von Deimling, Andreas; Katus, Hugo; Kulozik, Andreas; Korshunov, Andrey; Zitron, Edgar] Heidelberg Univ, Heidelberg, Germany. [Northcott, Paul; Taylor, Michael] Univ Toronto, Toronto, ON, Canada. [Ryzhova, Marina] NN Burdenko Inst Neurosurg, Moscow, Russia. [Coco, Simona; Tonini, Gian Paolo] Natl Canc Inst, Genoa, Italy. [Scheurlen, Wolfram] Nurnberg Childrens Hosp, Nurnberg, Germany. RI Coco, Simona/J-6051-2016; Katus, Hugo/P-1712-2016 OI Coco, Simona/0000-0002-5296-4325; NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1424 DI 10.1158/1538-7445.AM2012-1424 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501445 ER PT J AU Valle, BL D'Souza, T Becker, KG Wood, WH Wersto, RP Morin, PJ AF Valle, Blanca L. D'Souza, Theresa Becker, Kevin G. Wood, William H. Wersto, Robert P. Morin, Patrice J. TI The anti-proliferative effects of non-steroidal anti-inflammatory drugs (NSAIDs) diclofenac and indomethacin in ovarian cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Valle, Blanca L.; D'Souza, Theresa; Becker, Kevin G.; Wood, William H.; Wersto, Robert P.; Morin, Patrice J.] NIA, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4673 DI 10.1158/1538-7445.AM2012-4673 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603005 ER PT J AU Vathipadiekal, V Ozbun, L Lee, JW Vivas-Mejia, P Lopez-Berestein, G Sood, AK Mok, SC Birrer, MJ AF Vathipadiekal, Vinod Ozbun, Laurent Lee, Jeong-Won Vivas-Mejia, Pablo Lopez-Berestein, Gabriel Sood, Anil K. Mok, Samuel C. Birrer, Michael J. TI Gene expression profile of chemoresponse of microdissected papillary serous tumors of the ovary identifies POLH and REV3L as potential therapeutic targets SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Vathipadiekal, Vinod; Birrer, Michael J.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Ozbun, Laurent] NCI, Bethesda, MD 20892 USA. [Lee, Jeong-Won; Vivas-Mejia, Pablo; Lopez-Berestein, Gabriel; Sood, Anil K.; Mok, Samuel C.] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5057 DI 10.1158/1538-7445.AM2012-5057 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606259 ER PT J AU Vazquez-Ortiz, G Chisholm, CL Huang, RL Li, CL Xu, XL Rui-Hong, W Deng, CX AF Vazquez-Ortiz, Guelaguetza Chisholm, Cristine L. Huang, Ruili Li, Culling Xu, Xiaoling Rui-Hong, Wang Deng, Chuxia TI Identification of synergistic effect of autophagy inhibitors and resveratrol on BRCA1 mutant cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Vazquez-Ortiz, Guelaguetza; Chisholm, Cristine L.; Li, Culling; Xu, Xiaoling; Rui-Hong, Wang; Deng, Chuxia] GDDB NIDDK NIH, Bethesda, MD USA. [Huang, Ruili] NHGRI, Genom Ctr, NIH, Bethesda, MD 20892 USA. RI deng, chuxia/N-6713-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2744 DI 10.1158/1538-7445.AM2012-2744 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603166 ER PT J AU Vogtmann, E Li, HL Shu, XO Chow, WH Ji, BT Cai, H Gao, J Zhang, W Gao, YT Zheng, W Xiang, YB AF Vogtmann, Emily Li, Hong Lan Shu, Xiao Ou Chow, Wong Ho Ji, Bu Tian Cai, Hui Gao, Jing Zhang, Wei Gao, Yu Tang Zheng, Wei Xiang, Yong Bing TI Dietary glycemic load, glycemic index and risk of primary liver cancer: Results from the Shanghai women's and men's health studies SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Vogtmann, Emily; Li, Hong Lan; Gao, Jing; Zhang, Wei; Gao, Yu Tang; Xiang, Yong Bing] Shanghai Canc Inst, Shanghai, Peoples R China. [Shu, Xiao Ou; Cai, Hui; Zheng, Wei] Vanderbilt Univ, Nashville, TN 37235 USA. [Chow, Wong Ho; Ji, Bu Tian] NCI, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 626 DI 10.1158/1538-7445.AM2012-626 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602081 ER PT J AU Wang, P Dong, QZ Zhang, C Kuan, PF Liu, Y Jeck, WR Andersen, JB Auman, JT Hoskins, JM Kim, JW Cibulskis, K Getz, G Hunt, HV Thorgeirsson, SS Roberts, LR Ye, D Guan, KL Xiong, Y Qin, LX Chiang, DY AF Wang, Pu Dong, Qiong-Zhu Zhang, Chong Kuan, Pei -Fen Liu, Yufeng Jeck, William R. Andersen, Jesper B. Auman, James Todd Hoskins, Janelle M. Kim, Jin Woo Cibulskis, Kristian Getz, Gad Hunt, Heike V. Thorgeirsson, Snorri S. Roberts, Lewis R. Ye, Dan Guan, Kun-Liang Xiong, Yue Qin, Lun-Xiu Chiang, Derek Y. TI Mutations in isocitrate dehydrogenase 1 and 2 are associated with DNA hypermethylation in intrahepatic cholangiocarcinomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wang, Pu; Dong, Qiong-Zhu; Ye, Dan; Qin, Lun-Xiu] Fudan Univ, Shanghai 200433, Peoples R China. [Zhang, Chong; Kuan, Pei -Fen; Liu, Yufeng; Jeck, William R.; Auman, James Todd; Hoskins, Janelle M.; Hunt, Heike V.; Xiong, Yue; Chiang, Derek Y.] Univ N Carolina, Chapel Hill, NC USA. [Andersen, Jesper B.; Thorgeirsson, Snorri S.] NCI, Bethesda, MD 20892 USA. [Kim, Jin Woo] Wake Forest Univ, Winston Salem, NC 27109 USA. [Cibulskis, Kristian; Getz, Gad] Broad Inst, Cambridge, MA USA. [Roberts, Lewis R.] Mayo Clin, Rochester, MN USA. [Guan, Kun-Liang] Univ Calif San Diego, San Diego, CA 92103 USA. [Qin, Lun-Xiu] Zhongshan Hosp, Shanghai, Peoples R China. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1058 DI 10.1158/1538-7445.AM2012-1058 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605053 ER PT J AU Watkins, SK Hurwitz, AA AF Watkins, Stephanie K. Hurwitz, Arthur A. TI Prostate cancer-infiltrating mast cells suppress anti -tumor immunity through a TGF beta and IL-13 dependent mechanism SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Watkins, Stephanie K.; Hurwitz, Arthur A.] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3540 DI 10.1158/1538-7445.AM2011-3540 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500448 ER PT J AU Wei, JT Sanda, MG Thompson, IM Partin, A Feng, ZD Sokoll, L Groskopf, J Brown, E Lotan, Y Kibel, A Lin, D Taneja, S Viterbo, R Busby, E Bidair, M Kagan, J Srivastava, S AF Wei, John Thomas Sanda, Martin G. Thompson, Ian M. Partin, Alan Feng, Ziding Sokoll, Lori Groskopf, Jack Brown, Elissa Lotan, Yair Kibel, Adam Lin, Daniel Taneja, Samir Viterbo, Rosalia Busby, Erik Bidair, Mohamed Kagan, Jacob Srivastava, Sudhir TI The NCI Early Detection Research Network (EDRN) urinary PCA3 validation trial SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wei, John Thomas] Univ Michigan, Ann Arbor, MI 48109 USA. [Sanda, Martin G.] Harvard BID, Boston, MA USA. [Thompson, Ian M.] UTSA, San Antonio, TX USA. [Partin, Alan; Sokoll, Lori] Johns Hopkins, Baltimore, MD USA. [Feng, Ziding; Brown, Elissa] Fred Hutch Canc Res Ctr, Seattle, WA USA. [Groskopf, Jack] Genprobe, San Diego, CA USA. [Lotan, Yair] UTSW, Dallas, TX USA. [Kibel, Adam] Harvard, Boston, MA USA. [Lin, Daniel] Univ Washington, Seattle, WA 98195 USA. [Taneja, Samir] NYU, New York, NY USA. [Viterbo, Rosalia] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA. [Busby, Erik] Univ Alabama Birmingham, Birmingham, AL USA. [Bidair, Mohamed] San Diego Clin Trials, San Diego, CA USA. [Kagan, Jacob; Srivastava, Sudhir] NCI, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4451 DI 10.1158/1538-7445.AM2012-4451 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503240 ER PT J AU Wentzensen, NA Killian, K Adams, L Luhn, P Kloor, M Guido, R Yang, HP Garcia-Closas, M Brinton, L d'Ambrosio, L Lissowska, J Hewitt, S Meltzer, P Sherman, M AF Wentzensen, Nicolas A. Killian, Keith Adams, Lisa Luhn, Patricia Kloor, Matthias Guido, Richard Yang, Hannah P. Garcia-Closas, Montserrat Brinton, Louise d'Ambrosio, Lori Lissowska, Jolanta Hewitt, Stephen Meltzer, Paul Sherman, Mark TI DNA methylation profiles of endometrial cancer and benign endometrium suggest differences by mismatch repair status and possible early detection markers SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wentzensen, Nicolas A.; Luhn, Patricia; Yang, Hannah P.; Brinton, Louise; Sherman, Mark] NCI, DCEG, Bethesda, MD 20892 USA. [Killian, Keith; Adams, Lisa; Hewitt, Stephen; Meltzer, Paul] NCI, Bethesda, MD 20892 USA. [Kloor, Matthias] Heidelberg Univ, Heidelberg, Germany. [Guido, Richard; d'Ambrosio, Lori] UPMC Syst, Magee Womens Hosp, Pittsburgh, PA USA. [Garcia-Closas, Montserrat] Inst Canc Res, London SW3 6JB, England. [Lissowska, Jolanta] 6M Sklodowska Curie Mem Canc Ctr, Warsaw, Poland. [Lissowska, Jolanta] Inst Oncol, Warsaw, Poland. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5012 DI 10.1158/1538-7445.AM2012-5012 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605351 ER PT J AU Wheless, LE Kistner-Griffin, E Jorgensen, TJ Ruczinski, I Berthier-Schaad, Y Kessing, B Hoffman-Bolton, J Woodward, L Yao, Y Strickland, PT Kao, WHL Alani, RM Smith, MW Alberg, AJ AF Wheless, Lee E. Kistner-Griffin, Emily Jorgensen, Timothy J. Ruczinski, Ingo Berthier-Schaad, Yvette Kessing, Bailey Hoffman-Bolton, Judith Woodward, Lesley Yao, Yin Strickland, Paul T. Kao, W. H. Linda Alani, Rhoda M. Smith, Michael W. Alberg, Anthony J. TI A community-based study of nucleotide excision repair polymorphisms in relation to risk of non-melanoma skin cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wheless, Lee E.; Kistner-Griffin, Emily; Woodward, Lesley; Alberg, Anthony J.] Med Univ S Carolina, Charleston, SC 29425 USA. [Jorgensen, Timothy J.] Georgetown Univ, Washington, DC USA. [Ruczinski, Ingo; Berthier-Schaad, Yvette; Strickland, Paul T.; Kao, W. H. Linda] Johns Hopkins Univ, Baltimore, MD USA. [Kessing, Bailey; Smith, Michael W.] NCI, Lab Genom Divers, Frederick, MD 21701 USA. [Hoffman-Bolton, Judith] George W Comstock Ctr Publ Hlth Res & Prevent, Hagerstown, MD USA. [Yao, Yin] NIMH, Bethesda, MD 20892 USA. [Alani, Rhoda M.] Boston Univ, Boston, MA 02215 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2639 DI 10.1158/1538-7445.AM2012-2639 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602268 ER PT J AU Wiggins, KR Davis, V Phadke, D Shah, R Archer, TK AF Wiggins, Kimberly R. Davis, Valerie Phadke, Dhiral Shah, Ruchir Archer, Trevor K. TI Inhibition of the ubiquitin proteasome system differentially regulates glucocorticoid receptor-mediated transcriptional processes SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wiggins, Kimberly R.; Davis, Valerie; Archer, Trevor K.] NIEHS, Res Triangle Pk, NC 27709 USA. [Phadke, Dhiral; Shah, Ruchir] SRA Int Inc, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3928 DI 10.1158/1538-7445.AM2012-3928 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602123 ER PT J AU Willard, MD Wulur, IH Samuels, Y Barber, TD Gartner, J Parker, SCJ AF Willard, Melinda D. Wulur, Isabella H. Samuels, Yardena Barber, Thomas D. Gartner, Jared Parker, Stephen C. J. TI Somatic mutations in Pyk2 in melanoma alter protein activity, interactions and localization SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Willard, Melinda D.; Wulur, Isabella H.; Barber, Thomas D.] Eli Lilly & Co, Indianapolis, IN 46285 USA. [Samuels, Yardena; Gartner, Jared] NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. [Parker, Stephen C. J.] NHGRI, Pharmacol Res Associate Program, NIGMS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 100 DI 10.1158/1538-7445.AM2012-100 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605373 ER PT J AU Woditschka, S Palmieri, D Duchnowska, R Jassem, J Badve, S Sledge, GW Steeg, PS AF Woditschka, Stephan Palmieri, Diane Duchnowska, Renata Jassem, Jacek Badve, Sunil Sledge, George W. Steeg, Patricia S. TI Overexpression of RAD51 promotes brain metastases from breast cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Woditschka, Stephan] NCI, Canc Prevent Fellowship Program, Rockville, MD USA. [Palmieri, Diane; Steeg, Patricia S.] NCI, Womens Canc Sect, Mol Pharmacol Lab, Ctr Canc Res, Bethesda, MD 20892 USA. [Duchnowska, Renata] Mil Inst Med, Warsaw, Poland. [Jassem, Jacek] Med Univ Gdansk, Gdansk, Poland. [Badve, Sunil] Indiana Univ Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA. [Sledge, George W.] Indiana Univ Sch Med, Dept Med, Div Hematol Oncol, Indianapolis, IN 46202 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5306 DI 10.1158/1538-7445.AM2012-5306 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504079 ER PT J AU Wright, ME Albanes, D Shi, F Moser, A Virtamo, J Gann, P AF Wright, Margaret E. Albanes, Demetrius Shi, Fei Moser, Ann Virtamo, Jarmo Gann, Peter TI Serum phytanic acid and prostate cancer risk: Results from a nested case-control study SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wright, Margaret E.; Shi, Fei; Gann, Peter] Univ Illinois, Chicago, IL USA. [Albanes, Demetrius] NCI, Bethesda, MD 20892 USA. [Moser, Ann] Kennedy Krieger Inst, Baltimore, MD USA. [Virtamo, Jarmo] Natl Inst Hlth & Welf, Helsinki, Finland. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4463 DI 10.1158/1538-7445.AM2012-4463 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603291 ER PT J AU Wu, J Keng, V Patmore, DM Jousma, E Eaves, DW Johansson, G Schwartz, EB Fuchs, JR Dunn, S Lindquist, D Dombi, E Widemann, BC Stemmer-Rachamimov, AO Cancelas, JA Cripe, TP Largaespada, DA Ratner, N AF Wu, Jiangiang Keng, Vincent Patmore, Deanna M. Jousma, Edwin Eaves, David W. Johansson, Gunnar Schwartz, Eric B. Fuchs, James R. Dunn, Scott Lindquist, Diana Dombi, Eva Widemann, Brigitte C. Stemmer-Rachamimov, Anat O. Cancelas, Jose A. Cripe, Timothy P. Largaespada, David A. Ratner, Nancy TI An EGFR-STAT3 pathway promotes NF1 peripheral nerve tumorigenesis and transformation SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wu, Jiangiang; Patmore, Deanna M.; Jousma, Edwin; Eaves, David W.; Johansson, Gunnar; Dunn, Scott; Lindquist, Diana; Cancelas, Jose A.; Cripe, Timothy P.; Ratner, Nancy] Cincinnati Childrens Hosp, Cincinnati, OH USA. [Keng, Vincent; Largaespada, David A.] Univ Minnesota, Minneapolis, MN USA. [Schwartz, Eric B.; Fuchs, James R.] Ohio State Univ, Columbus, OH 43210 USA. [Dombi, Eva; Widemann, Brigitte C.] NCI, Bethesda, MD 20892 USA. [Stemmer-Rachamimov, Anat O.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Stemmer-Rachamimov, Anat O.] Harvard Univ, Sch Med, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2937 DI 10.1158/1538-7445.AM2012-2937 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602343 ER PT J AU Wu, SH Shu, XO Chow, WH Xiang, YB Zhang, XL Li, HL Cai, QY Milne, G Ji, BT Cai, H Rothman, N Gao, YT Zheng, W Yang, G AF Wu, Shenghui Shu, Xiao Ou Chow, Wong-Ho Xiang, Yong-Bing Zhang, Xianglan Li, Hong-Lan Cai, Qiuyin Milne, Ginger Ji, Bu-Tian Cai, Hui Rothman, Nathaniel Gao, Yu-Tang Zheng, Wei Yang, Gong TI Physical activity and inflammatory and oxidative status markers in Chinese women SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wu, Shenghui; Shu, Xiao Ou; Zhang, Xianglan; Cai, Qiuyin; Cai, Hui; Zheng, Wei; Yang, Gong] Vanderbilt Univ, Dept Med, Div Epidemiol, Nashville, TN USA. [Chow, Wong-Ho; Ji, Bu-Tian; Rothman, Nathaniel] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Bethesda, MD 20892 USA. [Xiang, Yong-Bing; Li, Hong-Lan; Gao, Yu-Tang] Shanghai Canc Inst, Shanghai, Peoples R China. [Milne, Ginger] Vanderbilt Univ, Dept Med, Div Clin Pharmacol, Nashville, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 627 DI 10.1158/1538-7445.AM2012-627 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602082 ER PT J AU Wu, YZ Lu, JM Antony, S Juhasz, A Liu, H Jiang, GJ Roy, K Doroshow, J AF Wu, Yongzhong Lu, Jiamo Antony, Smitha Juhasz, Agnes Liu, Han Jiang, Guojian Roy, Krishnendu Doroshow, James TI Interferon-alpha induced TLR4 is indispensable for the synergistic induction of Duox2/DuoxA2 by lipopolysacharide (LPS) and IFN-gamma in human pancreatic cancer cells SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Wu, Yongzhong; Lu, Jiamo; Antony, Smitha; Juhasz, Agnes; Liu, Han; Jiang, Guojian; Roy, Krishnendu; Doroshow, James] NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3094 DI 10.1158/1538-7445.AM2012-3094 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605121 ER PT J AU Xiao, CL Yaschenko, E Kimelman, M Rodarmer, K Sherry, S AF Xiao, Chunlin Yaschenko, Eugene Kimelman, Mikhail Rodarmer, Kurt Sherry, Stephen TI Evaluation of data compression strategies for genetic variation calling using next generation sequencing data in normal and tumor samples SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Xiao, Chunlin; Yaschenko, Eugene; Kimelman, Mikhail; Rodarmer, Kurt; Sherry, Stephen] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 3983 DI 10.1158/1538-7445.AM2012-3983 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605086 ER PT J AU Xiao, ZX Jiang, Q Willette-Brown, J Zhu, F Young, HA Lin, FC Burkett, S Datla, M Xi, SC Schrump, DS Wiltrout, RH Hu, YL AF Xiao, Zuoxiang Jiang, Qun Willette-Brown, Jami Zhu, Feng Young, Howard A. Lin, Fanching Burkett, Sandra Datla, Mahesh Xi, Sichuan Schrump, David S. Wiltrout, Robert H. Hu, Yinling TI IKK alpha inactivation predisposes to spontaneous lung squamous cell carcinomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Xiao, Zuoxiang; Jiang, Qun; Willette-Brown, Jami; Zhu, Feng; Young, Howard A.; Lin, Fanching; Burkett, Sandra; Datla, Mahesh; Xi, Sichuan; Schrump, David S.; Wiltrout, Robert H.; Hu, Yinling] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2560 DI 10.1158/1538-7445.AM2012-2560 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600252 ER PT J AU Xu, BY Basuli, F Wu, H Salima, A Opina, A Lane, K Griffiths, GL Jagoda, E Green, M Seidel, J Choyke, P AF Xu, Biying Basuli, F. Wu, H. Salima, A. Opina, A. Lane, K. Griffiths, G. L. Jagoda, E. Green, M. Seidel, J. Choyke, P. TI Long circulating F-18-labeled liposomes for PET imaging SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Xu, Biying; Basuli, F.; Wu, H.; Salima, A.; Opina, A.; Lane, K.; Griffiths, G. L.] NIH, Rockville, MD USA. [Jagoda, E.; Green, M.; Seidel, J.; Choyke, P.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-420 DI 10.1158/1538-7445.AM2012-LB-420 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501281 ER PT J AU Yamashita, T Honda, M Kawaguchi, K Takatori, H Sunakozaka, H Wang, XW Kaneko, S AF Yamashita, Taro Honda, Masao Kawaguchi, Kazunori Takatori, Hajime Sunakozaka, Hajime Wang, Xin W. Kaneko, Shuichi TI Distinct liver cancer stem cells defined by EpCAM and CD90 in human hepatocellular carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yamashita, Taro; Honda, Masao; Kawaguchi, Kazunori; Takatori, Hajime; Sunakozaka, Hajime; Kaneko, Shuichi] Kanazawa Univ, Sch Med, Kanazawa, Ishikawa 920, Japan. [Wang, Xin W.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5336 DI 10.1158/1538-7445.AM2012-5336 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504111 ER PT J AU Yan, S Thiele, CJ AF Yan, Shuang Thiele, Carol J. TI Validation of potential therapeutic targeting of IL-6/JAK/STAT3 pathway by AZD1480 in neuroblastoma and other pediatric tumors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yan, Shuang; Thiele, Carol J.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1440 DI 10.1158/1538-7445.AM2012-1440 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701501461 ER PT J AU Yang, CZ Iyer, R Ikejiri, B Lonser, R Zhuang, ZP AF Yang, Chunzhang Iyer, Rajiv Ikejiri, Barbara Lonser, Russell Zhuang, Zhengping TI Beta-catenin signaling initiates the activation of astrocytes and contributes to the pathogenesis of astrocytomas SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yang, Chunzhang; Iyer, Rajiv; Ikejiri, Barbara; Lonser, Russell; Zhuang, Zhengping] NINDS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4220 DI 10.1158/1538-7445.AM2012-4220 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504479 ER PT J AU Yang, G Shu, XO Chow, WH Zhang, XL Li, HL Ji, BT Cai, H Wu, SH Gao, YT Zheng, W AF Yang, Gong Shu, Xiao-Ou Chow, Wong-Ho Zhang, Xianglan Li, Hong-Lan Ji, Bu-Tian Cai, Hui Wu, Sheng-Hui Gao, Yu-Tang Zheng, Wei TI Soy food intake and risk of lung cancer: Evidence from the Shanghai Women's Health Study and a meta-analysis SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yang, Gong; Shu, Xiao-Ou; Zhang, Xianglan; Cai, Hui; Wu, Sheng-Hui; Zheng, Wei] Vanderbilt Univ, Sch Med, Nashville, TN 37212 USA. [Chow, Wong-Ho; Ji, Bu-Tian] NCI, Div Canc Epidemiol & Genet, NIH, DHHS, Bethesda, MD 20892 USA. [Li, Hong-Lan; Gao, Yu-Tang] Shanghai Canc Inst, Shanghai, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 646 DI 10.1158/1538-7445.AM2012-646 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602102 ER PT J AU Yang, ZY Lu, L Wang, S Van Dyke, T AF Yang, Zhenye Lu, Lucy Wang, Sophie Van Dyke, Terry TI Aurora-A kinase is required for rapid establishment of the mitotic checkpoint SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yang, Zhenye; Lu, Lucy; Wang, Sophie; Van Dyke, Terry] NCI, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2053 DI 10.1158/1538-7445.AM2012-2053 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701604320 ER PT J AU Yoo, SS Cerna, D Wilsker, D Teicher, BA Coleman, CN AF Yoo, Stephen S. Cerna, David Wilsker, Deborah Teicher, Beverly A. Coleman, C. Norman TI Comparison of radiosensitizing effects of several PARP inhibitors under clinical investigation SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yoo, Stephen S.; Teicher, Beverly A.; Coleman, C. Norman] NCI, NIH, Bethesda, MD 20892 USA. [Cerna, David; Wilsker, Deborah] SAIC Frederick Inc, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 1460 DI 10.1158/1538-7445.AM2012-1460 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701502071 ER PT J AU Zakharov, AD Kabirov, KK Nikolic, D Chen, L Mankovskaya, I van Breemen, RB Benbrook, DM Kapetanovic, IM Lyubimov, AV AF Zakharov, Alexander D. Kabirov, Kasim K. Nikolic, Dejan Chen, Lian Mankovskaya, Irina van Breemen, Richard B. Benbrook, Doris M. Kapetanovic, Izet M. Lyubimov, Alexander V. TI Analysis of a new chemopreventative agent SHetA2 and its metabolites in Beagle dog liver and plasma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zakharov, Alexander D.; Kabirov, Kasim K.; Mankovskaya, Irina; Lyubimov, Alexander V.] Univ Illinois, Toxicol Res Lab, Chicago, IL USA. [Nikolic, Dejan; Chen, Lian; van Breemen, Richard B.] Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL USA. [Benbrook, Doris M.] Univ Oklahoma, Dept Obstet & Gynecol, Oklahoma City, OK USA. [Benbrook, Doris M.] Univ Oklahoma, Dept Biochem & Mol Biol, Oklahoma City, OK USA. [Kapetanovic, Izet M.] NCI, Bethesda, MD 20892 USA. RI Chen, Lian/C-5948-2017 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4781 DI 10.1158/1538-7445.AM2012-4781 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701600040 ER PT J AU Zalazar, F De Luca, P Elguero, B Gardner, K Figg, WD Meiss, R Moiola, CP Cotignola, J Vazguez, ES De Siervi, A AF Zalazar, Florencia De Luca, Paola Elguero, Belen Gardner, Kevin Figg, William D. Meiss, Roberto Moiola, Cristian P. Cotignola, Javier Vazguez, Elba S. De Siervi, Adriana TI CPS49 and Flavopiridol: a new selective drug combination for advanced prostate cancer SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zalazar, Florencia; De Luca, Paola; Elguero, Belen; Moiola, Cristian P.; Cotignola, Javier; Vazguez, Elba S.; De Siervi, Adriana] Univ Buenos Aires, Buenos Aires, DF, Argentina. [Gardner, Kevin; Figg, William D.] NCI, NIH, Bethesda, MD 20892 USA. [Meiss, Roberto] Acad Nacl Med Buenos Aires, Buenos Aires, DF, Argentina. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2340 DI 10.1158/1538-7445.AM2012-2340 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701504291 ER PT J AU Zhang, H Mackall, CL AF Zhang, Hua Mackall, Crystal L. TI Identifying an IDO-expressing "fibrocyte" subset of myeloid suppressor cells in pediatric cancer patients SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhang, Hua; Mackall, Crystal L.] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-127 DI 10.1158/1538-7445.AM2012-LB-127 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500252 ER PT J AU Zhang, JY Thorgeirsson, SS Jessup, JM AF Zhang, Jingyu Thorgeirsson, Snorri S. Jessup, J. Milburn TI Allele-specific shRNA to Nanog family members inhibits 3-D growth of colorectal carcinoma through the intrinsic apoptotic pathway SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhang, Jingyu; Thorgeirsson, Snorri S.] NCI, Expt Carcinogenesis Lab, CCR, Bethesda, MD 20892 USA. [Jessup, J. Milburn] NCI, Canc Diag Prog, DCTD, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5646 DI 10.1158/1538-7445.AM2012-5646 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603311 ER PT J AU Zhang, SL Hong, B Kopelovich, L El-Deiry, WS AF Zhang, Shengliang Hong, Bo Kopelovich, Levy El-Deiry, Wafik S. TI A novel small molecule p53-pathway restoring compound suppresses colorectal cancer cells via p73 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhang, Shengliang; Hong, Bo; El-Deiry, Wafik S.] Penn State Hershey Canc Inst, Hershey, PA USA. [Kopelovich, Levy] NCI, Canc Prevent Div, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4828 DI 10.1158/1538-7445.AM2012-4828 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602310 ER PT J AU Zhang, SY Zhang, CY Huang, WC Lowery, FJ Huang, SY Aldape, KD Steeg, PS Yu, DH AF Zhang, Siyuan Zhang, Chenyu Huang, Wen-Chien Lowery, Frank J. Huang, Suyun Aldape, Kenneth D. Steeg, Patricia S. Yu, Dihua TI Combating breast cancer brain metastasis by targeting Src family kinases SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhang, Siyuan; Zhang, Chenyu; Huang, Wen-Chien; Lowery, Frank J.; Huang, Suyun; Aldape, Kenneth D.; Yu, Dihua] UT MD Anderson Canc Ctr, Houston, TX USA. [Steeg, Patricia S.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2974 DI 10.1158/1538-7445.AM2012-2974 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701505039 ER PT J AU Zhang, YW Regairaz, M Seiler, J Agama, K Doroshow, J Pommier, Y AF Zhang, Yongwei Regairaz, Marie Seiler, Jennifer Agama, Keli Doroshow, James Pommier, Yves TI Rationale for combining PARP inhibitors with topotecan or irinotecan: taking advantage of the alternative DNA repair pathways involving XPF-ERCC1 and PARP-TDP1 SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhang, Yongwei; Regairaz, Marie; Seiler, Jennifer; Agama, Keli; Doroshow, James; Pommier, Yves] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4691 DI 10.1158/1538-7445.AM2012-4691 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603088 ER PT J AU Zhao, F Hoechst, B Gamrekelashvili, J Ormandy, L Voigtlander, T Wedemeyer, H Ylaya, K Hewitt, S Wang, X Manns, M Korangy, F Greten, T AF Zhao, Fei Hoechst, Bastian Gamrekelashvili, Jaba Ormandy, Lars Voigtlaender, Torsten Wedemeyer, Heiner Ylaya, Kris Hewitt, Stephen Wang, Xin Manns, Michael Korangy, Firouzeh Greten, Tim TI CCR4+CCR6+Th17 cells suppress autologous CD8+T cell responses in patients with hepatocellular carcinoma SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhao, Fei; Gamrekelashvili, Jaba; Ylaya, Kris; Hewitt, Stephen; Wang, Xin; Korangy, Firouzeh; Greten, Tim] NCI, Bethesda, MD 20892 USA. [Hoechst, Bastian; Ormandy, Lars; Voigtlaender, Torsten; Wedemeyer, Heiner; Manns, Michael] Hannover Med Sch, Hannover, Germany. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 5412 DI 10.1158/1538-7445.AM2012-5412 PG 1 WC Oncology SC Oncology GA V43SQ UT WOS:000209701500485 ER PT J AU Zhao, XL Roessler, S Budhu, A Yu, ZP Jia, HL Ye, QH Qin, LX Tang, ZY Wang, XW AF Zhao, Xuelian Roessler, Stephanie Budhu, Anuradha Yu, Zhipeng Jia, Huliang Ye, Qinghai Qin, Lunxiu Tang, Zhaoyou Wang, Xin Wei TI An integrative genomic approach uncovers oncogenic roles of YY1-signaling in hepatocellular carcinoma with stem cell-like traits SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhao, Xuelian; Roessler, Stephanie; Budhu, Anuradha; Yu, Zhipeng; Wang, Xin Wei] NCI, NIH, Bethesda, MD 20892 USA. [Jia, Huliang; Ye, Qinghai; Qin, Lunxiu; Tang, Zhaoyou] Fudan Univ, Liver Canc Inst, Shanghai 200032, Peoples R China. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA LB-408 DI 10.1158/1538-7445.AM2012-LB-408 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605012 ER PT J AU Zheng, YL Ogundiran, TO Adebamowo, C Nathanson, KL Domchek, SM Rebbeck, TR Simon, MS John, EM Hennis, A Nemesure, B Wu, SY Leske, MC Ambs, S Niu, Q Zhang, J Cox, NJ Olopade, OI Huo, DZ AF Zheng, Yonglan Ogundiran, Temidayo O. Adebamowo, Clement Nathanson, Katherine L. Domchek, Susan M. Rebbeck, Timothy R. Simon, Michael S. John, Esther M. Hennis, Anselm Nemesure, Barbara Wu, Suh-Yuh Leske, M. Cristina Ambs, Stefan Niu, Qun Zhang, Jing Cox, Nancy J. Olopade, Olufunmilayo I. Huo, Dezheng TI Absence of association between common genetic polymorphisms in the TERT-CLPTM1L locus and breast cancer risk in women of African descent SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zheng, Yonglan; Niu, Qun; Zhang, Jing; Cox, Nancy J.; Olopade, Olufunmilayo I.; Huo, Dezheng] Univ Chicago, Chicago, IL 60637 USA. [Ogundiran, Temidayo O.] Univ Ibadan, Ibadan, Nigeria. [Adebamowo, Clement] Univ Maryland, Baltimore, MD 21201 USA. [Nathanson, Katherine L.; Domchek, Susan M.; Rebbeck, Timothy R.] Univ Penn, Philadelphia, PA 19104 USA. [Simon, Michael S.] Wayne State Univ, Detroit, MI USA. [John, Esther M.] Canc Prevent Inst Calif, Fremont, CA USA. [Hennis, Anselm] Univ W Indies, Bridgetown, Barbados. [Nemesure, Barbara; Wu, Suh-Yuh; Leske, M. Cristina] SUNY Stony Brook, Stony Brook, NY 11794 USA. [Ambs, Stefan] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2606 DI 10.1158/1538-7445.AM2012-2606 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701602230 ER PT J AU Zhu, B Khozoie, C Ferry, CH Markell, LM Bility, MT Blazanin, N Glick, AB Gonzalez, FJ AF Zhu, Bokai Khozoie, Combiz Ferry, Christina H. Markell, Lauren M. Bility, Moses T. Blazanin, Nicholas Glick, Adam B. Gonzalez, Frank J. TI Anti-oncogenic role of peroxisome proliferator-activated receptor-alpha/beta (PPAR delta/alpha) involves inhibition of mitosis and regulation of Hras1-induced senescence SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zhu, Bokai; Khozoie, Combiz; Ferry, Christina H.; Markell, Lauren M.; Bility, Moses T.; Blazanin, Nicholas; Glick, Adam B.] Penn State Univ, University Pk, PA 16802 USA. [Gonzalez, Frank J.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2172 DI 10.1158/1538-7445.AM2012-2172 PG 1 WC Oncology SC Oncology GA V43SR UT WOS:000209701606152 ER PT J AU Zoppoli, G Solier, S Reinhold, WC Liu, HF Connelly, JW Monks, A Shoemaker, RH Abaan, OD Davis, SR Piras, D Ballestrero, A Meltzer, PS Doroshow, JH Pommier, Y AF Zoppoli, Gabriele Solier, Stephanie Reinhold, William C. Liu, Hongfang Connelly, John W. Monks, Anne Shoemaker, Robert H. Abaan, Ogan D. Davis, Sean R. Piras, Daniela Ballestrero, Alberto Meltzer, Paul S. Doroshow, James H. Pommier, Yves TI CHEK2 (Chk2) endogenous activation is associated with p53 deficiency and downregulation of BRCA2 and Fanconi Anemia pathway gene members in the National Cancer Institute Anticancer Tumor Cell Line Panel (NCI-60) SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zoppoli, Gabriele; Piras, Daniela; Ballestrero, Alberto] Univ Genoa, Genoa, Italy. [Solier, Stephanie; Reinhold, William C.; Abaan, Ogan D.; Davis, Sean R.; Meltzer, Paul S.; Doroshow, James H.; Pommier, Yves] NIH, Bethesda, MD 20892 USA. [Liu, Hongfang] Mayo Clin, Div Biomed Stat & Infomat, Rochester, MN USA. [Connelly, John W.; Monks, Anne; Shoemaker, Robert H.] NIH, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 2116 DI 10.1158/1538-7445.AM2012-2116 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701605160 ER PT J AU Zoppoli, G Regairaz, M Leo, E Reinhold, WC Pommier, Y AF Zoppoli, Gabriele Regairaz, Marie Leo, Elisabetta Reinhold, William C. Pommier, Yves TI The putative DNA/RNA Helicase Schlafen-11 sensitizes cancer cells to topoisomerase I inhibitors SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Zoppoli, Gabriele] Univ Genoa, Genoa, Italy. [Regairaz, Marie; Leo, Elisabetta; Reinhold, William C.; Pommier, Yves] NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 4693 DI 10.1158/1538-7445.AM2012-4693 PG 2 WC Oncology SC Oncology GA V43SR UT WOS:000209701603090 ER PT J AU Zhu, XM Ahmad, SM Aboukhalil, A Busser, BW Kim, Y Tansey, TR Haimovich, A Jeffries, N Bulyk, ML Michelson, AM AF Zhu, Xianmin Ahmad, Shaad M. Aboukhalil, Anton Busser, Brian W. Kim, Yongsok Tansey, Terese R. Haimovich, Adrian Jeffries, Neal Bulyk, Martha L. Michelson, Alan M. TI Differential regulation of mesodermal gene expression by Drosophila cell type-specific Forkhead transcription factors SO DEVELOPMENT LA English DT Article DE Transcription factors; Transcription factor binding sites; Forkhead proteins; Transcriptional regulation; Enhancers; Mesoderm ID VISCERAL MESODERM; DIRECT TARGET; BETA-3-TUBULIN GENE; HEART DEVELOPMENT; DORSAL VESSEL; TINMAN; PROTEIN; MELANOGASTER; INTEGRATION; BINIOU AB A common theme in developmental biology is the repeated use of the same gene in diverse spatial and temporal domains, a process that generally involves transcriptional regulation mediated by multiple separate enhancers, each with its own arrangement of transcription factor (TF)-binding sites and associated activities. Here, by contrast, we show that the expression of the Drosophila Nidogen (Ndg) gene at different embryonic stages and in four mesodermal cell types is governed by the binding of multiple cell-specific Forkhead (Fkh) TFs - including Biniou (Bin), Checkpoint suppressor homologue (CHES-1-like) and Jumeau (Jumu) - to three functionally distinguishable Fkh-binding sites in the same enhancer. Whereas Bin activates the Ndg enhancer in the late visceral musculature, CHES-1-like cooperates with Jumu to repress this enhancer in the heart. CHES-1-like also represses the Ndg enhancer in a subset of somatic myoblasts prior to their fusion to form multinucleated myotubes. Moreover, different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF. A genome-wide scan for the occurrence of both classes of Fkh domain recognition sites in association with binding sites for known cardiac TFs showed an enrichment of combinations containing the two Fkh motifs in putative enhancers found within the noncoding regions of genes having heart expression. Collectively, our results establish that different cell-specific members of a TF family regulate the activity of a single enhancer in distinct spatiotemporal domains, and demonstrate how individual binding motifs for a TF class can differentially influence gene expression. C1 [Zhu, Xianmin; Ahmad, Shaad M.; Busser, Brian W.; Kim, Yongsok; Tansey, Terese R.; Haimovich, Adrian; Michelson, Alan M.] NHLBI, Lab Dev Syst Biol, Genet & Dev Biol Ctr, NIH, Bethesda, MD 20892 USA. [Aboukhalil, Anton; Bulyk, Martha L.] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA. [Aboukhalil, Anton; Bulyk, Martha L.] Harvard Univ, Sch Med, Boston, MA 02115 USA. [Aboukhalil, Anton] MIT, Dept Aeronaut & Astronaut, Cambridge, MA 02139 USA. [Jeffries, Neal] NHLBI, Off Biostat Res, NIH, Bethesda, MD 20892 USA. [Bulyk, Martha L.] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA. [Bulyk, Martha L.] Harvard Univ, Sch Med, Harvard MIT Div Hlth Sci & Technol HST, Boston, MA 02115 USA. RP Michelson, AM (reprint author), NHLBI, Lab Dev Syst Biol, Genet & Dev Biol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA. EM michelsonam@mail.nih.gov OI Ahmad, Shaad/0000-0001-5373-1710 FU National Heart, Lung, and Blood Institute (NHLBI) Division of Intramural Research; National Institutes of Health (NIH) [R01 HG005287-01A1]; American Heart Association FX This work was supported by the National Heart, Lung, and Blood Institute (NHLBI) Division of Intramural Research (A.M.M.), by the National Institutes of Health (NIH) [R01 HG005287-01A1 to M.L.B.], by an American Heart Association Predoctoral Fellowship (A.A.) and by an American Heart Association Postdoctoral Fellowship (S.M.A.). Deposited in PMC for immediate release. NR 66 TC 13 Z9 14 U1 0 U2 3 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0950-1991 J9 DEVELOPMENT JI Development PD APR 15 PY 2012 VL 139 IS 8 BP 1457 EP 1466 DI 10.1242/dev.069005 PG 10 WC Developmental Biology SC Developmental Biology GA 914JG UT WOS:000301945100011 PM 22378636 ER PT J AU Porras, C Wentzensen, N Rodriguez, AC Morales, J Burk, RD Alfaro, M Hutchinson, M Herrero, R Hildesheim, A Sherman, ME Wacholder, S Solomon, D Schiffman, M AF Porras, Carolina Wentzensen, Nicolas Rodriguez, Ana C. Morales, Jorge Burk, Robert D. Alfaro, Mario Hutchinson, Martha Herrero, Rolando Hildesheim, Allan Sherman, Mark E. Wacholder, Sholom Solomon, Diane Schiffman, Mark TI Switch from cytology-based to human papillomavirus test-based cervical screening: implications for colposcopy SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE HPV testing; cytology; colposcopy; cervical cancer screening; cervicography ID INTRAEPITHELIAL NEOPLASIA; INTEROBSERVER AGREEMENT; PREDICTIVE VALUES; FOLLOW-UP; HIGH-RISK; CANCER; DNA; WOMEN; STRATEGIES; INFECTION AB Human papillomavirus (HPV) testing is more sensitive than cytology; some cervical cancer prevention programs will switch from cytology to carcinogenic HPV test-based screening. The objective of our study is to evaluate the clinical implications of a switch to HPV test-based screening on performance and workload of colposcopy. Women in the population-based, 7-year Guanacaste cohort study were screened at enrollment using cytology. We also took another specimen for HPV DNA testing and collected magnified cervical photographic images (cervigrams). A final case diagnosis (>= cervical intraepithelial neoplasia [CIN] grade 3, CIN2, = CIN2 and >= CIN3. A program based on immediate colposcopic referral after positive HPV would immediately identify as high risk more of the cumulative >= CIN2 cases than conventional cytology, because of an increased number of referrals. However, the proportion of women that would have visible lesions at referral to colposcopy and the sensitivity versus specificity trade-off of the colposcopic impressions would be similar to programs using cytology (>= atypical squamous cells of unknown significance [ASCUS]) for referral. The major concern with switching from cytology to more sensitive HPV screening is management of the many HPV-positive women, including those with still nonvisible >= CIN2 lesions. Our data support the need for a nonvisual diagnostic method to guide management and treatment of HPV-positive women. C1 [Porras, Carolina; Rodriguez, Ana C.; Morales, Jorge; Alfaro, Mario; Herrero, Rolando] Fdn INCIENSA, Proyecto Epidemiol Guanacaste, San Jose, Costa Rica. [Porras, Carolina; Wentzensen, Nicolas; Hildesheim, Allan; Sherman, Mark E.; Wacholder, Sholom; Solomon, Diane; Schiffman, Mark] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. [Burk, Robert D.] Albert Einstein Coll Med, Albert Einstein Canc Ctr, Bronx, NY 10467 USA. [Hutchinson, Martha] Brown Univ, Women & Infants Hosp, Providence, RI USA. RP Porras, C (reprint author), Fdn INCIENSA, Proyecto Epidemiol Guanacaste, 300 Mts Oeste ICE Piso 7, San Jose, Costa Rica. EM cporras@proyectoguanacaste.org RI perumal, murugiah/D-1565-2012; Hildesheim, Allan/B-9760-2015 OI Hildesheim, Allan/0000-0003-0257-2363 FU National Institutes of Health [N01-CP-21081, N01-CP-33061, N01-CP-40542, N01-CP-50535, N01-CP-81023, CA78527]; National Cancer Institute, National Institutes of Health, Department of Health and Human Services FX Grant sponsor: National Institutes of Health; Grant numbers: N01-CP-21081, N01-CP-33061, N01-CP-40542, N01-CP-50535 and N01-CP-81023; Grant sponsor: Intramural Program of NIH; Grant number: CA78527; Grant sponsors: National Cancer Institute, National Institutes of Health, Department of Health and Human Services and Senior Fellowship Program, National Institutes of Health NR 26 TC 9 Z9 12 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2012 VL 130 IS 8 BP 1879 EP 1887 DI 10.1002/ijc.26194 PG 9 WC Oncology SC Oncology GA 897WV UT WOS:000300692300018 PM 21607948 ER PT J AU Guech-Ongey, M Yagi, M Palacpac, NMQ Emmanuel, B Talisuna, AO Bhatia, K Stefan, DC Biggar, RJ Nkrumah, F Neequaye, J Tougan, T Horii, T Mbulaiteye, SM AF Guech-Ongey, Mercy Yagi, Masanori Palacpac, Nirianne Marie Q. Emmanuel, Benjamin Talisuna, Ambrose O. Bhatia, Kishor Stefan, D. Cristina Biggar, Robert J. Nkrumah, Francis Neequaye, Janet Tougan, Takahiro Horii, Toshihiro Mbulaiteye, Sam M. TI Antibodies reactive to Plasmodium falciparum serine repeat antigen in children with Burkitt lymphoma from Ghana SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE Plasmodium falciparum malaria; Africa; Epstein-Barr virus; immunity; epidemiology ID EPSTEIN-BARR-VIRUS; MALARIA; SERA; IMMUNITY; STABILITY; INFECTION; CORRELATE; PROTEINS; UGANDA AB The role of protective immunity to Plasmodium falciparum (Pf) malaria in Burkitt lymphoma (BL) is unknown. We investigated the association between BL and antibodies reactive to SE36 antigen, a recombinant protein based on P. falciparum serine repeat antigen 5 gene, targeted by protective malaria immune responses. Cases were children (0-14 years) enrolled at the Korle-Bu Teaching Hospital, Accra, Ghana, during 1965-1994 with BL confirmed by histology or cytology (92% of cases). Controls were apparently healthy children enrolled contemporaneous to the cases from the nearest neighbor house to the case house and were age,- sex-frequency-matched to the cases. Anti-SE36 IgG antibodies were measured using enzyme-linked absorbent immunoassays (ELISAs). SE36 titers were estimated by extrapolating ELISA optical density readings to a standard fitting curve. Anti-SE36 titers were log-transformed for analysis. Odds ratios (ORs) and two-sided 95% confidence intervals (95% CIs) were estimated using unconditional logistic regression. The mean log endpoint dilution titers were 0.63 logs lower in cases than in controls (8.26 [SD 1.68] vs. 8.89 [SD 1.75], Student's t-test, p = 0.019). Lower titers were observed in cases than controls aged 0-4 years (p = 0.05) and in those aged 5-14 years (p = 0.06). Low and medium tertiles of anti-SE36 IgG antibodies were associated with increased OR for BL ([OR 1.67, 95% CI 1.21-2.31] and [OR 1.33, 95% CI 0.96-1.86], respectively, p(trend) = 0.002) in analyses adjusting for age, sex, calendar period and test plate. Our findings suggest that compared to similarly aged children enrolled from the same community, children with BL in Ghana have lower antibodies to SE36 antigen. C1 [Guech-Ongey, Mercy; Emmanuel, Benjamin; Bhatia, Kishor; Mbulaiteye, Sam M.] NCI, Div Canc Epidemiol & Genet, DCEG, Bethesda, MD 20892 USA. [Yagi, Masanori; Palacpac, Nirianne Marie Q.; Tougan, Takahiro; Horii, Toshihiro] Osaka Univ, Microbial Dis Res Inst, Osaka, Japan. [Talisuna, Ambrose O.] Uganda Minist Hlth, Infect Dis Res Collaborat, Kampala, Uganda. [Stefan, D. Cristina] Univ Stellenbosch, Dept Pediat & Child Hlth, Cape Town, South Africa. [Nkrumah, Francis] Univ Ghana, Noguchi Mem Inst, Legon, Ghana. [Neequaye, Janet] Korle Bu Univ Teaching Hosp Accra, Dept Child Hlth, Accra, Ghana. RP Mbulaiteye, SM (reprint author), 6120 Execut Blvd,Execut Plaza S,Room 7080,MSC 724, Rockville, MD 20852 USA. EM mbulaits@mail.nih.gov FU National Cancer Institute, National Institutes of Health, Department of Health and Human Services [N01-CO-12400]; Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan FX Grant sponsor: National Cancer Institute, National Institutes of Health, Department of Health and Human Services (Intramural Research Program of the Division of Cancer Epidemiology and Genetics); Support Services Contract number: N01-CO-12400; Grant sponsor: Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Biomedical Kansai project supported by the Regional Innovation Cluster Program) NR 30 TC 4 Z9 4 U1 0 U2 1 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2012 VL 130 IS 8 BP 1908 EP 1914 DI 10.1002/ijc.26203 PG 7 WC Oncology SC Oncology GA 897WV UT WOS:000300692300021 PM 21630256 ER PT J AU Farsaci, B Higgins, JP Hodge, JW AF Farsaci, Benedetto Higgins, Jack P. Hodge, James W. TI Consequence of dose scheduling of sunitinib on host immune response elements and vaccine combination therapy SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE sunitinib; vaccine; immunotherapy; TKI; cancer ID RENAL-CELL CARCINOMA; TYROSINE KINASE INHIBITOR; HUMAN CARCINOEMBRYONIC-ANTIGEN; SUPPRESSOR-CELLS; TUMOR MICROENVIRONMENT; CANCER; IDENTIFICATION; SORAFENIB; REVERSAL; MALATE AB Our study investigated the immunomodulatory effects of sunitinib to rationally design combinational platforms with immunotherapies for the treatment of solid tumors. Using a mouse model, we studied the effects of sunitinib given for 4 weeks at concentrations comparable to 37.5-50 mg/day in humans, followed by 2 weeks off the drug (sunitinib 4/2). We assessed the effect of differently timed combinations of sunitinib and a poxvirus-based vaccine encoding carcinoembryonic antigen (CEA) plus 3 costimulatory molecules on immune responses in CEA-transgenic (CEA-Tg) mice. Antitumor studies were performed in CEA-Tg mice bearing CEA-transfected MC38 murine colon carcinomas (MC38-CEA), treated either concurrently or sequentially with sunitinib and vaccine. In vitro, sunitinib inhibited PDGFR phosphorylation on MC38-CEA cells at concentrations similar to those biologically available during human treatment. In vivo, one cycle of sunitinib 4/2 caused bimodal immune effects: (a) decreased regulatory cells during the 4 weeks of treatment and (b) an immune-suppression rebound during the 2 weeks of treatment interruption. In a model using CEA-Tg mice bearing CEA(+) tumors, continuous sunitinib followed by vaccine increased intratumoral infiltration of antigen-specific T lymphocytes, decreased immunosuppressant T regulatory cells and myeloid-derived suppressor cells, reduced tumor volumes and increased survival. The immunomodulatory activity of continuous sunitinib administration can create a more immune-permissive environment. In combination with immunotherapies, sunitinib treatment should precede vaccine, to precondition the immune system, to maximize the response to vaccine-mediated immune enhancement. C1 [Farsaci, Benedetto; Higgins, Jack P.; Hodge, James W.] NCI, Lab Tumor Immunol & Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Hodge, JW (reprint author), NCI, Lab Tumor Immunol & Biol, Ctr Canc Res, NIH, 10 Ctr Dr,Room 8B09, Bethesda, MD 20892 USA. EM jh241d@nih.gov RI Farsaci, Benedetto/L-9837-2014; Hodge, James/D-5518-2015 OI Farsaci, Benedetto/0000-0001-8275-2561; Hodge, James/0000-0001-5282-3154 FU Center for Cancer Research, National Cancer Institute, NIH FX Grant sponsor: Intramural Research Program of the Center for Cancer Research, National Cancer Institute, NIH NR 32 TC 53 Z9 53 U1 0 U2 4 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD APR 15 PY 2012 VL 130 IS 8 BP 1948 EP 1959 DI 10.1002/ijc.26219 PG 12 WC Oncology SC Oncology GA 897WV UT WOS:000300692300025 PM 21633954 ER PT J AU Jahouh, F Hou, SJ Kovac, P Banoub, JH AF Jahouh, Farid Hou, Shu-jie Kovac, Pavol Banoub, Joseph H. TI Determination of glycation sites by tandem mass spectrometry in a synthetic lactose-bovine serum albumin conjugate, a vaccine model prepared by dialkyl squarate chemistry SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID VIBRIO-CHOLERAE O-1; SEROTYPE OGAWA; O-PS; DIETHYL SQUARATE; PROTEINS; OLIGOSACCHARIDES; NOMENCLATURE; PEPTIDES; PROGRESS; SPECTRA AB RATIONALE: Neoglycoconjugate vaccines synthesized by the squaric acid spacer method allow single point attachment of the carbohydrate antigen to the protein carrier. However, the localization of the carbohydrate antigen sites of conjugation on the protein carrier has been an elusive task difficult to achieve. METHOD: Covalent attachment of the lactose antigen to the bovine serum albumin (BSA) was prepared by the squaric acid method using a hapten: BSA ratio of 20: 1. Different reaction times were used during the conjugation reaction and two different lactose-BSA glycoconjugate vaccines were obtained. The carbohydrate antigen hapten: BSA ratios of these lactose-BSA glycoconjugate vaccines were determined by MALDI-TOF/ RTOF-MS and the glycation sites in the neoglycoconjugates were determined using nano-LC/ ESI-QqTOF-MS/ MS analysis of the trypsin and GluC V8 digests of the conjugates. RESULTS: We have identified a total of 15 glycation sites located on the BSA lysine residues for the neoglycoconjugate vaccine formed with a hapten: BSA ratio of 5.1: 1, However, the tryptic and GluC V8 digests of the hapten-BSA glycoconjugate with a hapten: BSA ratio of 19.0: 1 allowed identification of 30 glycation sites located on the BSA. These last results seem to indicate that this conjugation results in formation of various glycoforms. CONCLUSIONS: It was observed that the number of identified glycation sites increasedwhen the hapten: BSA ratio of glycoconjugate formation increased, and that the location of the glycation sites appears to be mainly on the outer surface of the BSA carrier molecule which is in line with the assumption that the sterically more accessible lysine residues, namely those located on the outer surface of the BSA, would be conjugated preferentially. Copyright (C)2012 John Wiley & Sons, Ltd. C1 [Banoub, Joseph H.] Dept Fisheries & Oceans Canada, Sci Branch, Special Projects, St John, NF A1C 5X1, Canada. [Jahouh, Farid; Banoub, Joseph H.] Mem Univ Newfoundland, Dept Chem, St John, NF A1B 3X7, Canada. [Hou, Shu-jie; Kovac, Pavol] NIDDK, LBC, NIH, Bethesda, MD 20892 USA. RP Banoub, JH (reprint author), Dept Fisheries & Oceans Canada, Sci Branch, Special Projects, St John, NF A1C 5X1, Canada. EM banoubjo@dfo-mpo.gc.ca FU Intramural NIH HHS [ZIA DK059701-38] NR 24 TC 6 Z9 6 U1 2 U2 14 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0951-4198 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PD APR 15 PY 2012 VL 26 IS 7 BP 749 EP 758 DI 10.1002/rcm.6166 PG 10 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA 898EO UT WOS:000300719100005 PM 22368054 ER PT J AU Saunders, RC Vann, SD Aggleton, JP AF Saunders, Richard C. Vann, Seralynne D. Aggleton, John P. TI Projections from Gudden's tegmental nuclei to the mammillary body region in the cynomolgus monkey (Macaca fascicularis) SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE amnesia; head-direction; hypothalamus; memory; primate; raphe nucleus; tegmentum ID HORSERADISH-PEROXIDASE; EFFERENT CONNECTIONS; SUPRAMAMMILLARY NUCLEUS; HIPPOCAMPAL-FORMATION; ANTERIOR THALAMUS; PREFRONTAL CORTEX; MACAQUE MONKEYS; CEREBRAL-CORTEX; MAMILLARY BODY; RHESUS-MONKEY AB Gudden's tegmental nuclei provide major inputs to the rodent mammillary bodies, where they are thought to be important for learning and navigation. Comparable projections have yet to be described in the primate brain, where part of the problem has been in effectively delineating these nuclei. Immunohistochemical staining of tissue from a series of macaque monkeys (Macaca mulatta) showed that cells in the region of both the ventral and dorsal tegmental nuclei selectively stain for parvalbumin, thus helping to reveal these nuclei. These same tegmental nuclei were not selectively revealed when tissue was stained for SMI32, acetylcholinesterase, calbindin, or calretinin. In a parallel study, horseradish peroxidase was injected into the mammillary bodies of five cynomolgus monkeys (Macaca fascicularis). Retrogradely labeled neurons were consistently found in the three subdivisions of the ventral tegmental nucleus of Gudden, which are located immediately below, within, and above the medial longitudinal fasciculus. Further projections to the mammillary body region arose from cells in the anterior tegmental nucleus, which appears to be a rostral continuation of the infrafascicular part of the ventral tegmental nucleus. In the dorsal tegmental nucleus of Gudden, labeled cells were most evident when the tracer injection was more laterally placed in the mammillary bodies, consistent with a projection to the lateral mammillary nucleus. The present study not only demonstrates that the primate mammillary bodies receive parallel inputs from the dorsal and ventral tegmental nuclei of Gudden, but also helps to confirm the extent of these poorly distinguished nuclei in the monkey brain. J. Comp. Neurol. 520:11281145, 2012. (C) 2011 Wiley Periodicals, Inc. C1 [Vann, Seralynne D.; Aggleton, John P.] Cardiff Univ, Sch Psychol, Cardiff CF10 3AT, S Glam, Wales. [Saunders, Richard C.] NIMH, Neuropsychol Lab, Bethesda, MD 20892 USA. RP Aggleton, JP (reprint author), Cardiff Univ, Sch Psychol, Pk Pl, Cardiff CF10 3AT, S Glam, Wales. EM aggleton@cf.ac.uk RI Vann, Seralynne/A-1601-2010; OI Vann, Seralynne/0000-0002-6709-8773; Aggleton, John/0000-0002-5573-1308 FU Royal Society; Wolfson Trust; National Institute of Mental Health FX Grant sponsors: The Royal Society, the Wolfson Trust, National Institute of Mental Health Intramural Research Program. NR 59 TC 11 Z9 11 U1 1 U2 3 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD APR 15 PY 2012 VL 520 IS 6 BP 1128 EP 1145 DI 10.1002/cne.22740 PG 18 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA 887RV UT WOS:000299947900002 PM 21830220 ER PT J AU Weinberger, DM Malley, R Lipsitch, M AF Weinberger, Daniel M. Malley, Richard Lipsitch, Marc TI Serotype replacement after pneumococcal vaccination Reply SO LANCET LA English DT Letter ID DISEASE C1 [Weinberger, Daniel M.; Lipsitch, Marc] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA. [Malley, Richard] Childrens Hosp, Div Infect Dis, Dept Med, Boston, MA 02115 USA. [Malley, Richard] Harvard Univ, Sch Med, Boston, MA 02115 USA. [Weinberger, Daniel M.] NIH, Div Int Epidemiol & Populat Studies, Fogarty Int Ctr, Bethesda, MD 20892 USA. RP Weinberger, DM (reprint author), Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, 665 Huntington Ave, Boston, MA 02115 USA. EM dweinber@gmail.com NR 4 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0140-6736 J9 LANCET JI Lancet PD APR 14 PY 2012 VL 379 IS 9824 BP 1388 EP 1389 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 926BR UT WOS:000302806800020 ER PT J AU Bhardwaj, G Ko, KD Hong, YJ Zhang, ZH Ho, NL Chintapalli, SV Kline, LA Gotlin, M Hartranft, DN Patterson, ME Dave, F Smith, EJ Holmes, EC Patterson, RL van Rossum, DB AF Bhardwaj, Gaurav Ko, Kyung Dae Hong, Yoojin Zhang, Zhenhai Ho, Ngai Lam Chintapalli, Sree V. Kline, Lindsay A. Gotlin, Matthew Hartranft, David Nicholas Patterson, Morgen E. Dave, Foram Smith, Evan J. Holmes, Edward C. Patterson, Randen L. van Rossum, Damian B. TI PHYRN: A Robust Method for Phylogenetic Analysis of Highly Divergent Sequences SO PLOS ONE LA English DT Article ID MULTIPLE ALIGNMENT; TWILIGHT ZONE; MIXED MODELS; PROTEIN; TREES; EVOLUTION; RECONSTRUCTION; INFERENCE; DANGER; MATRIX AB Both multiple sequence alignment and phylogenetic analysis are problematic in the "twilight zone" of sequence similarity (<= 25% amino acid identity). Herein we explore the accuracy of phylogenetic inference at extreme sequence divergence using a variety of simulated data sets. We evaluate four leading multiple sequence alignment (MSA) methods (MAFFT, T-COFFEE, CLUSTAL, and MUSCLE) and six commonly used programs of tree estimation (Distance-based: Neighbor-Joining; Character-based: PhyML, RAxML, GARLI, Maximum Parsimony, and Bayesian) against a novel MSA-independent method (PHYRN) described here. Strikingly, at "midnight zone" genetic distances (similar to 7% pairwise identity and 4.0 gaps per position), PHYRN returns high-resolution phylogenies that outperform traditional approaches. We reason this is due to PHRYN's capability to amplify informative positions, even at the most extreme levels of sequence divergence. We also assess the applicability of the PHYRN algorithm for inferring deep evolutionary relationships in the divergent DANGER protein superfamily, for which PHYRN infers a more robust tree compared to MSA-based approaches. Taken together, these results demonstrate that PHYRN represents a powerful mechanism for mapping uncharted frontiers in highly divergent protein sequence data sets. C1 [Bhardwaj, Gaurav; Ko, Kyung Dae; Hong, Yoojin; Zhang, Zhenhai; Ho, Ngai Lam; Kline, Lindsay A.; Gotlin, Matthew; Hartranft, David Nicholas; Patterson, Morgen E.; Dave, Foram; Smith, Evan J.; Patterson, Randen L.; van Rossum, Damian B.] Penn State Univ, Ctr Computat Prote, University Pk, PA 16802 USA. [Bhardwaj, Gaurav; Ko, Kyung Dae; Hong, Yoojin; Kline, Lindsay A.; Gotlin, Matthew; Hartranft, David Nicholas; Smith, Evan J.; Holmes, Edward C.; van Rossum, Damian B.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Hong, Yoojin; Ho, Ngai Lam] Penn State Univ, Dept Comp Sci & Engn, University Pk, PA 16802 USA. [Zhang, Zhenhai] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Holmes, Edward C.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Bhardwaj, Gaurav; Patterson, Randen L.] Univ Calif Davis, Dept Biochem & Mol Med, Sch Med, Davis, CA 95616 USA. [Chintapalli, Sree V.; Patterson, Randen L.] Univ Calif Davis, Dept Physiol & Membrane Biol, Sch Med, Davis, CA 95616 USA. [Bhardwaj, Gaurav; Chintapalli, Sree V.; Patterson, Randen L.; van Rossum, Damian B.] Univ Calif Davis, Ctr Translat Biosci & Comp, Davis, CA 95616 USA. RP Bhardwaj, G (reprint author), Penn State Univ, Ctr Computat Prote, University Pk, PA 16802 USA. EM randen100@gmail.com; dbv10@psu.edu OI Patterson, Morgen/0000-0003-4660-2755; Holmes, Edward/0000-0001-9596-3552 FU Searle Young Investigators Award; PSU; NCSA [TG-MCB070027N]; National Science Foundation [428-15 691M]; National Institutes of Health [R01 GM087410-01]; Fellowship from the Eberly College of Sciences; Huck Institutes of the Life Sciences; Pennsylvania Department of Health using Tobacco Settlement Funds FX This work was supported by the Searle Young Investigators Award and start-up money from PSU (RLP), NCSA grant TG-MCB070027N (RLP, DVR), The National Science Foundation 428-15 691M (RLP, DVR), and The National Institutes of Health R01 GM087410-01 (RLP, DVR). This project was also funded by a Fellowship from the Eberly College of Sciences and the Huck Institutes of the Life Sciences (DVR) and a grant with the Pennsylvania Department of Health using Tobacco Settlement Funds (DVR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 8 Z9 8 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 13 PY 2012 VL 7 IS 4 AR e34261 DI 10.1371/journal.pone.0034261 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UT UT WOS:000305341600032 PM 22514627 ER PT J AU Zajdowicz, S Haller, JC Krafft, AE Hunsucker, SW Mant, CT Duncan, MW Hodges, RS Jones, DNM Holmes, RK AF Zajdowicz, Sheryl Haller, Jon C. Krafft, Amy E. Hunsucker, Steve W. Mant, Colin T. Duncan, Mark W. Hodges, Robert S. Jones, David N. M. Holmes, Randall K. TI Purification and Structural Characterization of Siderophore (Corynebactin) from Corynebacterium diphtheriae SO PLOS ONE LA English DT Article ID MICROBIAL IRON TRANSPORT; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; COORDINATION CHEMISTRY; BACILLUS-SUBTILIS; STAPHYLOFERRIN-A; GENE-CLUSTER; GLUTAMICUM; NMR; BIOSYNTHESIS AB During infection, Corynebacterium diphtheriae must compete with host iron-sequestering mechanisms for iron. C. diphtheriae can acquire iron by a siderophore-dependent iron-uptake pathway, by uptake and degradation of heme, or both. Previous studies showed that production of siderophore (corynebactin) by C. diphtheriae is repressed under high-iron growth conditions by the iron-activated diphtheria toxin repressor (DtxR) and that partially purified corynebactin fails to react in chemical assays for catecholate or hydroxamate compounds. In this study, we purified corynebactin from supernatants of low-iron cultures of the siderophore-overproducing, DtxR-negative mutant strain C. diphtheriae C7(beta) Delta dtxR by sequential anion-exchange chromatography on AG1-X2 and Source 15Q resins, followed by reverse-phase high-performance liquid chromatography (RP-HPLC) on Zorbax C8 resin. The Chrome Azurol S (CAS) chemical assay for siderophores was used to detect and measure corynebactin during purification, and the biological activity of purified corynebactin was shown by its ability to promote growth and iron uptake in siderophore-deficient mutant strains of C. diphtheriae under iron-limiting conditions. Mass spectrometry and NMR analysis demonstrated that corynebactin has a novel structure, consisting of a central lysine residue linked through its alpha- and epsilon-amino groups by amide bonds to the terminal carboxyl groups of two different citrate residues. Corynebactin from C. diphtheriae is structurally related to staphyloferrin A from Staphylococcus aureus and rhizoferrin from Rhizopus microsporus in which D-ornithine or 1,4-diaminobutane, respectively, replaces the central lysine residue that is present in corynebactin. C1 [Zajdowicz, Sheryl; Haller, Jon C.; Holmes, Randall K.] Univ Colorado, Sch Med, Dept Microbiol, Aurora, CO USA. [Zajdowicz, Sheryl] Metropolitan State Coll Denver, Dept Biol, Denver, CO USA. [Krafft, Amy E.] NIAID, Resp Dis Branch, Div Microbiol & Infect Dis, NIH,Dept Hlth & Human Serv, Bethesda, MD 20892 USA. [Hunsucker, Steve W.; Duncan, Mark W.] Univ Colorado, Sch Med, Dept Endocrinol Metab & Diabet, Aurora, CO USA. [Mant, Colin T.; Hodges, Robert S.] Univ Colorado, Sch Med, Dept Biochem & Mol Genet, Aurora, CO USA. [Jones, David N. M.] Univ Colorado, Sch Med, Dept Pharmacol, Aurora, CO USA. RP Zajdowicz, S (reprint author), Univ Colorado, Sch Med, Dept Microbiol, Aurora, CO USA. EM Randall.Holmes@ucdenver.edu RI Jones, David/C-5764-2013 FU NIH [R37-AI14107, RO1-GM061855]; John Stewart Endowed Chair in Peptide Chemistry; University of Colorado Cancer Center Support Grant [NIH P30-CA046934] FX This work was supported in part by NIH grant R37-AI14107 (to RKH) and NIH grant RO1-GM061855 and the John Stewart Endowed Chair in Peptide Chemistry (to RSH). Operation of the NMR spectrometers was supported by the Program in Structural Biology and Biophysics and the University of Colorado Cancer Center Support Grant (NIH P30-CA046934). No other grants contributed to support of this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 6 Z9 6 U1 1 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 13 PY 2012 VL 7 IS 4 AR e34591 DI 10.1371/journal.pone.0034591 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UT UT WOS:000305341600047 PM 22514641 ER PT J AU Dong, HT Zhang, L Zheng, KL Yao, HG Chen, J Yu, FC Yu, XX Mao, BZ Zhao, D Yao, J Li, DB AF Dong, Hai-Tao Zhang, Lu Zheng, Kang-Le Yao, Hai-Gen Chen, Jack Yu, Feng-Chi Yu, Xiao-Xing Mao, Bi-Zeng Zhao, Dong Yao, Jian Li, De-Bao TI A Gaijin-like miniature inverted repeat transposable element is mobilized in rice during cell differentiation SO BMC GENOMICS LA English DT Article ID ARABIDOPSIS-THALIANA; LTR-RETROTRANSPOSONS; ANTHER CULTURE; DNA TRANSPOSON; GENOME; MITES; EVOLUTION; FAMILY; REVEALS; GENES AB Background: Miniature inverted repeat transposable element (MITE) is one type of transposable element (TE), which is largely found in eukaryotic genomes and involved in a wide variety of biological events. However, only few MITEs were proved to be currently active and their physiological function remains largely unknown. Results: We found that the amplicon discrepancy of a gene locus LOC_Os01g0420 in different rice cultivar genomes was resulted from the existence of a member of Gaijin-like MITEs (mGing). This result indicated that mGing transposition was occurred at this gene locus. By using a modified transposon display (TD) analysis, the active transpositions of mGing were detected in rice Jiahua No. 1 genome under three conditions: in seedlings germinated from the seeds received a high dose gamma-ray irradiation, in plantlets regenerated from anther-derived calli and from scutellum-derived calli, and were confirmed by PCR validation and sequencing. Sequence analysis revealed that single nucleotide polymorphisms (SNPs) or short additional DNA sequences at transposition sites post mGing transposition. It suggested that sequence modification was possibly taken place during mGing transposition. Furthermore, cell re-differentiation experiment showed that active transpositions of both mGing and mPing (another well studied MITE) were identified only in regenerated plantlets. Conclusions: It is for the first time that mGing active transposition was demonstrated under gamma-ray irradiation or in cell re-differentiation process in rice. This newly identified active MITE will provide a foundation for further analysis of the roles of MITEs in biological process. C1 [Dong, Hai-Tao; Zhang, Lu; Yu, Xiao-Xing; Mao, Bi-Zeng; Zhao, Dong; Li, De-Bao] Zhejiang Univ, Coll Agr & Biotechnol, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China. [Zheng, Kang-Le] China Natl Rice Res Inst, Natl Ctr Rice Improvement, Hangzhou 310006, Zhejiang, Peoples R China. [Yao, Hai-Gen; Yu, Feng-Chi; Yao, Jian] Jiaxing Acad Agr Sci, Jiaxing 314016, Peoples R China. [Chen, Jack] NICHD, Unit Biocomputat, NIH, Bethesda, MD 20892 USA. RP Li, DB (reprint author), Zhejiang Univ, Coll Agr & Biotechnol, Inst Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China. EM debl3@yahoo.com FU National Natural Science Foundation of China [30871329]; Natural Science Foundation of Zhejiang [Y307099] FX The authors would like to thank Dr. Lunquan Sun for his critical reading and valuable suggestions for improvement of the manuscript and Dr. Frederick Luk for his help and contribution in preparing the manuscript. This work was supported in part by grants from the National Natural Science Foundation of China grant 30871329 and Natural Science Foundation of Zhejiang grant Y307099. The funding agencies played no role in study design, in the collection, analysis and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication. NR 32 TC 7 Z9 8 U1 0 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 13 PY 2012 VL 13 AR 135 DI 10.1186/1471-2164-13-135 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 943WK UT WOS:000304156200001 PM 22500940 ER PT J AU Zeng, HK Shen, EH Hohmann, JG Oh, SW Bernard, A Royall, JJ Glattfelder, KJ Sunkin, SM Morris, JA Guillozet-Bongaarts, AL Smith, KA Ebbert, AJ Swanson, B Kuan, L Page, DT Overly, CC Lein, ES Hawrylycz, MJ Hof, PR Hyde, TM Kleinman, JE Jones, AR AF Zeng, Hongkui Shen, Elaine H. Hohmann, John G. Oh, Seung Wook Bernard, Amy Royall, Joshua J. Glattfelder, Katie J. Sunkin, Susan M. Morris, John A. Guillozet-Bongaarts, Angela L. Smith, Kimberly A. Ebbert, Amanda J. Swanson, Beryl Kuan, Leonard Page, Damon T. Overly, Caroline C. Lein, Ed S. Hawrylycz, Michael J. Hof, Patrick R. Hyde, Thomas M. Kleinman, Joel E. Jones, Allan R. TI Large-Scale Cellular-Resolution Gene Profiling in Human Neocortex Reveals Species-Specific Molecular Signatures SO CELL LA English DT Article ID HUMAN BRAIN EVOLUTION; CEREBRAL-CORTEX; HUMAN GENOME; HOMO-SAPIENS; COPY NUMBER; MOUSE-BRAIN; EXPRESSION; NEURONS; TRANSCRIPTOME; INSIGHTS AB Although there have been major advances in elucidating the functional biology of the human brain, relatively little is known of its cellular and molecular organization. Here we report a large-scale characterization of the expression of similar to 1,000 genes important for neural functions by in situ hybridization at a cellular resolution in visual and temporal cortices of adult human brains. These data reveal diverse gene expression patterns and remarkable conservation of each individual gene's expression among individuals (95%), cortical areas (84%), and between human and mouse (79%). A small but substantial number of genes (21%) exhibited species-differential expression. Distinct molecular signatures, comprised of genes both common between species and unique to each, were identified for each major cortical cell type. The data suggest that gene expression profile changes may contribute to differential cortical function across species, and in particular, a shift from corticosubcortical to more predominant corticocortical communications in the human brain. C1 [Zeng, Hongkui; Shen, Elaine H.; Hohmann, John G.; Oh, Seung Wook; Bernard, Amy; Royall, Joshua J.; Glattfelder, Katie J.; Sunkin, Susan M.; Morris, John A.; Guillozet-Bongaarts, Angela L.; Smith, Kimberly A.; Ebbert, Amanda J.; Swanson, Beryl; Kuan, Leonard; Page, Damon T.; Overly, Caroline C.; Lein, Ed S.; Hawrylycz, Michael J.; Jones, Allan R.] Allen Inst Brain Sci, Seattle, WA 98103 USA. [Hof, Patrick R.] Mt Sinai Sch Med, Fishberg Dept Neurosci, New York, NY 10029 USA. [Hof, Patrick R.] Mt Sinai Sch Med, Friedman Brain Inst, New York, NY 10029 USA. [Hyde, Thomas M.] Lieber Inst Brain Dev, Baltimore, MD 21205 USA. [Hyde, Thomas M.; Kleinman, Joel E.] NIMH, Sect Neuropathol, Clin Brain Disorders Branch,NIH, Genes Cognit & Psychosis Program,Intramural Res P, Bethesda, MD 20892 USA. RP Zeng, HK (reprint author), Allen Inst Brain Sci, Seattle, WA 98103 USA. EM hongkuiz@alleninstitute.org; allanj@alleninstitute.org FU Allen Institute for Brain Science; Allen Institute FX We are grateful for the technical support and expertise of the Atlas Production Team, led by Paul Wohnoutka, and the Information Technology Team, led by Chinh Dang, at the Allen Institute, without which the work would have not been accomplished. We thank Drs. Deborah Mash and Margaret Basile of the University of Miami Brain Endowment Bank for providing tissue. This work was funded by the Allen Institute for Brain Science. The authors wish to thank the Allen Institute founders, Paul G. Allen and Jody Allen, for their vision, encouragement, and support. NR 46 TC 79 Z9 79 U1 1 U2 24 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 J9 CELL JI Cell PD APR 13 PY 2012 VL 149 IS 2 BP 483 EP 496 DI 10.1016/j.cell.2012.02.052 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 926BF UT WOS:000302805600024 PM 22500809 ER PT J AU Murai, J Huang, SYN Das, BB Dexheimer, TS Takeda, S Pommier, Y AF Murai, Junko Huang, Shar-yin N. Das, Benu Brata Dexheimer, Thomas S. Takeda, Shunichi Pommier, Yves TI Tyrosyl-DNA Phosphodiesterase 1 (TDP1) Repairs DNA Damage Induced by Topoisomerases I and II and Base Alkylation in Vertebrate Cells SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID DOUBLE-STRAND BREAKS; CLEAVAGE COMPLEXES; SPINOCEREBELLAR ATAXIA; COVALENT COMPLEXES; REPLICATION FORKS; AXONAL NEUROPATHY; ANTITUMOR DRUGS; TRANSCRIPTION; ENZYME; PHOSPHORYLATION AB Tyrosyl-DNA phosphodiesterase 1 (Tdp1) repairs topoisomerase I cleavage complexes (Top1cc) by hydrolyzing their 3'-phosphotyrosyl DNA bonds and repairs bleomycin-induced DNA damage by hydrolyzing 3'-phosphoglycolates. Yeast Tdp1 has also been implicated in the repair of topoisomerase II-DNA cleavage complexes (Top2cc). To determine whether vertebrate Tdp1 is involved in the repair of various DNA end-blocking lesions, we generated Tdp1 knock-out cells in chicken DT40 cells (Tdp1(-/-)) and Tdp1-complemented DT40 cells with human TDP1. We found that Tdp1(-/-) cells were not only hypersensitive to camptothecin and bleomycin but also to etoposide, methyl methanesulfonate (MMS), H2O2, and ionizing radiation. We also show they were deficient in mitochondrial Tdp1 activity. In biochemical assays, recombinant human TDP1 was found to process 5'-phosphotyrosyl DNA ends when they mimic the 5'-overhangs of Top2cc. Tdp1 also processes 3'-deoxyribose phosphates generated from hydrolysis of abasic sites, which is consistent with the hypersensitivity of Tdp1(-/-) cells to MMS and H2O2. Because recent studies established that CtIP together with BRCA1 also repairs topoisomerase-mediated DNA damage, we generated dual Tdp1-CtIP-deficient DT40 cells. Our results show that Tdp1 and CtIP act in parallel pathways for the repair of Top1cc and MMS-induced lesions but are epistatic for Top2cc. Together, our findings reveal a broad involvement of Tdp1 in DNA repair and clarify the role of human TDP1 in the repair of Top2-induced DNA damage. C1 [Huang, Shar-yin N.; Das, Benu Brata; Dexheimer, Thomas S.; Pommier, Yves] NCI, Mol Pharmacol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Murai, Junko; Takeda, Shunichi] Kyoto Univ, Dept Radiat Genet, Grad Sch Med, Sakyo Ku, Kyoto 6068501, Japan. RP Pommier, Y (reprint author), NCI, Mol Pharmacol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM pommier@nih.gov FU National Institutes of Health through NCI Center for Cancer Research; Japan Society for the Promotion of Science FX This work was supported, in whole or in part, by the National Institutes of Health through the Intramural Program of the NCI Center for Cancer Research.; Recipient of a fellowship from the Japan Society for the Promotion of Science. NR 67 TC 53 Z9 55 U1 0 U2 21 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 13 PY 2012 VL 287 IS 16 BP 12848 EP 12857 DI 10.1074/jbc.M111.333963 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 927JO UT WOS:000302903700024 PM 22375014 ER PT J AU Geczy, T Peach, ML El Kazzouli, S Sigano, DM Kang, JH Valle, CJ Selezneva, J Woo, W Kedei, N Lewin, NE Garfield, SH Lim, L Mannan, P Marquez, VE Blumberg, PM AF Geczy, Tamas Peach, Megan L. El Kazzouli, Said Sigano, Dina M. Kang, Ji-Hye Valle, Christopher J. Selezneva, Julia Woo, Wonhee Kedei, Noemi Lewin, Nancy E. Garfield, Susan H. Lim, Langston Mannan, Poonam Marquez, Victor E. Blumberg, Peter M. TI Molecular Basis for Failure of "Atypical" C1 Domain of Vav1 to Bind Diacylglycerol/Phorbol Ester SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROTEIN-KINASE-C; CONFORMATIONALLY CONSTRAINED ANALOGS; DROSOPHILA-MELANOGASTER HOMOLOG; SIGNAL TRANSDUCER PROTEIN; SITE-DIRECTED MUTAGENESIS; CYSTEINE-RICH DOMAINS; PHORBOL ESTER; BIOLOGICAL-ACTIVITIES; HUMAN MALIGNANCIES; CRYSTAL-STRUCTURE AB C1 domains, the recognition motif of the second messenger diacylglycerol and of the phorbol esters, are classified as typical (ligand-responsive) or atypical (not ligand-responsive). The C1 domain of Vav1, a guanine nucleotide exchange factor, plays a critical role in regulation of Vav activity through stabilization of the Dbl homology domain, which is responsible for exchange activity of Vav. Although the C1 domain of Vav1 is classified as atypical, it retains a binding pocket geometry homologous to that of the typical C1 domains of PKCs. This study clarifies the basis for its failure to bind ligands. Substituting Vav1-specific residues into the C1b domain of PKC delta, we identified five crucial residues (Glu(9), Glu(10), Thr(11), Thr(24), and Tyr(26)) along the rim of the binding cleft that weaken binding potency in a cumulative fashion. Reciprocally, replacing these incompatible residues in the Vav1 C1 domain with the corresponding residues from PKC delta C1b (delta C1b) conferred high potency for phorbol ester binding. Computer modeling predicts that these unique residues in Vav1 increase the hydrophilicity of the rim of the binding pocket, impairing membrane association and thereby preventing formation of the ternary C1-ligand-membrane binding complex. The initial design of diacylglycerol-lactones to exploit these Vav1 unique residues showed enhanced selectivity for C1 domains incorporating these residues, suggesting a strategy for the development of ligands targeting Vav1. C1 [Geczy, Tamas; Valle, Christopher J.; Selezneva, Julia; Woo, Wonhee; Kedei, Noemi; Lewin, Nancy E.; Blumberg, Peter M.] NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Garfield, Susan H.; Lim, Langston; Mannan, Poonam] NCI, Expt Carcinogenesis Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [El Kazzouli, Said; Sigano, Dina M.; Kang, Ji-Hye; Marquez, Victor E.] NCI, Biol Chem Lab, Mol Discovery Program, Ctr Canc Res,NIH, Ft Detrick, MD 21702 USA. [Peach, Megan L.] NCI, Basic Res Program, SAIC Frederick, NIH, Ft Detrick, MD 21702 USA. RP Blumberg, PM (reprint author), NCI, Lab Canc Biol & Genet, Ctr Canc Res, NIH, Bldg 37,Rm 4048,37 Convent Dr,MSC 4255, Bethesda, MD 20892 USA. EM blumberp@dc37a.nci.nih.gov RI Sigano, Dina/M-6144-2014 OI Sigano, Dina/0000-0001-7489-9555 FU National Institutes of Health from NCI [Z1A BC 005270] FX This work was supported, in whole or in part, by National Institutes of Health Project Z1A BC 005270 from the Intramural Research Program, NCI. NR 69 TC 11 Z9 11 U1 0 U2 10 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 13 PY 2012 VL 287 IS 16 BP 13137 EP 13158 DI 10.1074/jbc.M111.320010 PG 22 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 927JO UT WOS:000302903700053 PM 22351766 ER PT J AU Joe, MK Kee, C Tomarev, SI AF Joe, Myung Kuk Kee, Changwon Tomarev, Stanislav I. TI Myocilin Interacts with Syntrophins and Is Member of Dystrophin-associated Protein Complex SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID OPEN-ANGLE GLAUCOMA; TRABECULAR MESHWORK CELLS; SKELETAL-MUSCLE ATROPHY; GLUCOCORTICOID-INDUCED MYOPATHY; GLYCOPROTEIN COMPLEX; TRANSCRIPTION FACTORS; GENE-EXPRESSION; TRANSGENIC MICE; MUTATED MOUSE; NON-SECRETION AB Genetic studies have linked myocilin to open angle glaucoma, but the functions of the protein in the eye and other tissues have remained elusive. The purpose of this investigation was to elucidate myocilin function(s). We identified alpha 1-syntrophin, a component of the dystrophin-associated protein complex (DAPC), as a myocilin-binding candidate. Myocilin interacted with alpha 1-syntrophin via its N-terminal domain and co-immunoprecipitated with alpha 1-syntrophin from C2C12 myotubes and mouse skeletal muscle. Expression of 15-fold higher levels of myocilin in the muscles of transgenic mice led to the elevated association of alpha 1-syntrophin, neuronal nitric-oxide synthase, and alpha-dystroglycan with DAPC, which increased the binding of laminin to alpha-dystroglycan and Akt signaling. Phosphorylation of Akt and Forkhead box O-class 3, key regulators of muscle size, was increased more than 3-fold, whereas the expression of muscle-specific RING finger protein-1 and atrogin-1, muscle atrophy markers, was decreased by 79 and 88%, respectively, in the muscles of transgenic mice. Consequently, the average size of muscle fibers of the transgenic mice was increased by 36% relative to controls. We suggest that intracellular myocilin plays a role as a regulator of muscle hypertrophy pathways, acting through the components of DAPC. C1 [Joe, Myung Kuk; Tomarev, Stanislav I.] NEI, Retinal Gangl Cell Biol Sect, Lab Retinal Cell & Mol Biol, NIH, Bethesda, MD 20892 USA. [Kee, Changwon] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Ophthalmol, Seoul 135710, South Korea. RP Tomarev, SI (reprint author), NEI, Retinal Gangl Cell Biol Sect, Lab Retinal Cell & Mol Biol, NIH, Bldg 6,Rm 212A,6 Ctr Dr, Bethesda, MD 20892 USA. EM tomarevs@nei.nih.gov FU National Institutes of Health, NEI FX This work was supported, in whole or in part, by the National Institutes of Health, NEI, Intramural Research Program. NR 66 TC 12 Z9 13 U1 0 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 13 PY 2012 VL 287 IS 16 BP 13216 EP 13227 DI 10.1074/jbc.M111.224063 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 927JO UT WOS:000302903700059 PM 22371502 ER PT J AU Walmacq, C Cheung, ACM Kireeva, ML Lubkowska, L Ye, CC Gotte, D Strathern, JN Care, T Cramer, P Kashlev, M AF Walmacq, Celine Cheung, Alan C. M. Kireeva, Maria L. Lubkowska, Lucyna Ye, Chengcheng Gotte, Deanna Strathern, Jeffrey N. Care, Thomas Cramer, Patrick Kashlev, Mikhail TI Mechanism of Translesion Transcription by RNA Polymerase II and Its Role in Cellular Resistance to DNA Damage SO MOLECULAR CELL LA English DT Article ID CYCLOBUTANE PYRIMIDINE DIMER; STRUCTURAL BASIS; EXCISION-REPAIR; THYMINE DIMER; TRIGGER LOOP; DHFR GENE; A-RULE; ELONGATION; FIDELITY; COMPLEX AB UV-induced cyclobutane pyrimidine dimers (CPDs) in the template DNA strand stall transcription elongation by RNA polymerase II (Pol II). If the nucleotide excision repair machinery does not promptly remove the CPDs, stalled Pol II creates a roadblock for DNA replication and subsequent rounds of transcription. Here we present evidence that Pol II has an intrinsic capacity for translesion synthesis (TLS) that enables bypass of the CPD with or without repair. Trans lesion synthesis depends on the trigger loop and bridge helix, the two flexible regions of the Pol II subunit Rpb1 that participate in substrate binding, catalysis, and translocation. Substitutions in Rpb1 that promote lesion bypass in vitro increase UV resistance in vivo, and substitutions that inhibit lesion bypass decrease cell survival after UV irradiation. Thus, translesion transcription becomes essential for cell survival upon accumulation of the unrepaired CPD lesions in genomic DNA. C1 [Walmacq, Celine; Kireeva, Maria L.; Lubkowska, Lucyna; Ye, Chengcheng; Gotte, Deanna; Strathern, Jeffrey N.; Kashlev, Mikhail] NCI, Ctr Canc Res, Frederick, MD 21702 USA. [Cheung, Alan C. M.; Cramer, Patrick] Univ Munich, Gene Ctr, D-81377 Munich, Germany. [Cheung, Alan C. M.; Cramer, Patrick] Univ Munich, Dept Biochem, Ctr Integrated Prot Sci CIPSM, D-81377 Munich, Germany. [Care, Thomas] Univ Munich, Dept Chem, Ctr Integrated Prot Sci CIPSM, D-81377 Munich, Germany. RP Kashlev, M (reprint author), NCI, Ctr Canc Res, Frederick, MD 21702 USA. EM mkashlev@mail.ncifcrf.gov OI Carell, Thomas/0000-0001-7898-2831 FU Deutsche Forschungsgemeinschaft [SFB646, TR5, FOR1068]; NIM; Bioimaging Network BIN; Jung-Stiftung FX We thank Brenda Schafer for technical help and Alison Rattray and Donald Court for helpful discussion and for critical reading of the manuscript. This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute. The contents of this publication do not necessarily reveal the views of the policy of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government. Part of this work was performed at the Swiss Light Source at the Paul Scherrer Institut, Villigen, Switzerland. P.C. was supported by the Deutsche Forschungsgemeinschaft, SFB646, TR5, FOR1068, NIM, Bioimaging Network BIN, and the Jung-Stiftung. NR 39 TC 47 Z9 47 U1 3 U2 15 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell PD APR 13 PY 2012 VL 46 IS 1 BP 18 EP 29 DI 10.1016/j.molcel.2012.02.006 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 926NL UT WOS:000302838000005 PM 22405652 ER PT J AU Li, MM He, YL Dubois, W Wu, XL Shi, JX Huang, J AF Li, Mangmang He, Yunlong Dubois, Wendy Wu, Xiaolin Shi, Jianxin Huang, Jing TI Distinct Regulatory Mechanisms and Functions for p53-Activated and p53-Repressed DNA Damage Response Genes in Embryonic Stem Cells SO MOLECULAR CELL LA English DT Article ID WILD-TYPE P53; TRANSCRIPTIONAL REPRESSION; PLURIPOTENT; NETWORK; GENOME; EXPRESSION; PATHWAY; STATE; METHYLATION; SIGNATURES AB p53 is critical in regulating the differentiation of ES and induced pluripotent stem (iPS) cells. Here, we report a whole-genome study of p53-mediated DNA damage signaling in mouse ES cells. Systems analyses reveal that binding of p53 at the promoter region significantly correlates with gene activation but not with repression. Unexpectedly, we identify a regulatory mode for p53-mediated repression through interfering with distal enhancer activity. Importantly, many ES cell-enriched core transcription factors are p53-repressed genes. Further analyses demonstrate that p53-repressed genes are functionally associated with ES/iPS cell status while p53-activated genes are linked to differentiation. p53-activated genes and -repressed genes also display distinguishable features of expression levels and epigenetic markers. Upon DNA damage, p53 regulates the self-renewal and pluripotency of ES cells. Together, these results support a model where, in response to DNA damage, p53 affects the status of ES cells through activating differentiation-associated genes and repressing ES cell-enriched genes. C1 [Li, Mangmang; He, Yunlong; Dubois, Wendy; Huang, Jing] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. [Wu, Xiaolin] NCI, Lab Mol Technol, Sci Applicat Int Corp Frederick Inc, Frederick, MD 21702 USA. [Shi, Jianxin] NCI, Biostat Branch, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. RP Huang, J (reprint author), NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. EM huangj3@mail.nih.gov RI Huang, Jing/A-2566-2009; LI, MANGMANG/J-1683-2015; He, Yunlong/D-1278-2017 OI Huang, Jing/0000-0002-7163-5156; FU Office of Science and Technology Partnerships at the Center for Cancer Research (CCR); National Cancer Institute (NCI) at the National Institutes of Health (NIH); NCI; National Cancer Institute, National Institutes of Health [HHSN261200800001E] FX We thank Glenn Merlino, Tom Misteli, Nan Roche, and Li Guo for critically reading the manuscript and for their comments. J.H.'s laboratory was funded by the intramural research program and partially by the Office of Science and Technology Partnerships at the Center for Cancer Research (CCR), the National Cancer Institute (NCI) at the National Institutes of Health (NIH), and the NCI Director's Innovation Award (to J.S. and J.H.). Microarray study has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The computational analyses utilized the high-performance computational capabilities of the Helix Systems at NIH (http://helix.nih.gov). NR 42 TC 95 Z9 95 U1 3 U2 16 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell PD APR 13 PY 2012 VL 46 IS 1 BP 30 EP 42 DI 10.1016/j.molcel.2012.01.020 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 926NL UT WOS:000302838000006 PM 22387025 ER PT J AU De Muyt, A Jessop, L Kolar, E Sourirajan, A Chen, JH Dayani, Y Lichten, M AF De Muyt, Arnaud Jessop, Lea Kolar, Elizabeth Sourirajan, Anuradha Chen, Jianhong Dayani, Yaron Lichten, Michael TI BLM Helicase Ortholog Sgs1 Is a Central Regulator of Meiotic Recombination Intermediate Metabolism SO MOLECULAR CELL LA English DT Article ID DOUBLE-STRAND-BREAK; DOUBLE HOLLIDAY JUNCTIONS; BLOOMS-SYNDROME HELICASE; SACCHAROMYCES-CEREVISIAE; CROSSING-OVER; BUDDING YEAST; HOMOLOGOUS RECOMBINATION; MUS81/MMS4 ENDONUCLEASE; JOINT MOLECULES; RECQ HELICASE AB The BLM helicase has been shown to maintain genome stability by preventing accumulation of aberrant recombination intermediates. We show here that the Saccharomyces cerevisiae BLM ortholog, Sgs1, plays an integral role in normal meiotic recombination, beyond its documented activity limiting aberrant recombination intermediates. In wild-type meiosis, temporally and mechanistically distinct pathways produce crossover and noncrossover recombinants. Crossovers form late in meiosis I prophase, by polo kinase-triggered resolution of Holliday junction (HJ) intermediates. Noncrossovers form earlier, via processes that do not involve stable HJ intermediates. In contrast, sgs1 mutants abolish early noncrossover formation. Instead, both non-crossovers and crossovers form by late HJ intermediate resolution, using an alternate pathway requiring the overlapping activities of Mus81-Mms4, Yen1, and Slx1-Slx4, nucleases with minor roles in wildtype meiosis. We conclude that Sgs1 is a primary regulator of recombination pathway choice during meiosis and suggest a similar function in the mitotic cell cycle. C1 [De Muyt, Arnaud; Jessop, Lea; Kolar, Elizabeth; Sourirajan, Anuradha; Chen, Jianhong; Dayani, Yaron; Lichten, Michael] NCI, Lab Biochem & Mol Biol, Ctr Canc Res, Bethesda, MD 20892 USA. RP Lichten, M (reprint author), NCI, Lab Biochem & Mol Biol, Ctr Canc Res, Bethesda, MD 20892 USA. EM lichten@helix.nih.gov RI Lichten, Michael/C-5795-2013 OI Lichten, Michael/0000-0001-9707-2956 FU National Institutes of Health, through the Center for Cancer Research of the National Cancer Institute FX We thank Neil Hunter for communicating unpublished data, and Valerie Borde, Dhruba Chattoraj, Mathilde Grelon, Neil Hunter, Raphael Mercier, Yikang Rong, and Denise Zickler for helpful discussions. This work was supported by the Intramural Research Program of the National Institutes of Health, through the Center for Cancer Research of the National Cancer Institute. NR 66 TC 82 Z9 82 U1 6 U2 23 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell PD APR 13 PY 2012 VL 46 IS 1 BP 43 EP 53 DI 10.1016/j.molcel.2012.02.020 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 926NL UT WOS:000302838000007 PM 22500736 ER PT J AU Lee, IH Kawai, Y Fergusson, MM Rovira, II Bishop, AJR Motoyama, N Cao, L Finkel, T AF Lee, In Hye Kawai, Yoshichika Fergusson, Maria M. Rovira, Ilsa I. Bishop, Alexander J. R. Motoyama, Noboru Cao, Liu Finkel, Toren TI Atg7 Modulates p53 Activity to Regulate Cell Cycle and Survival During Metabolic Stress SO SCIENCE LA English DT Article ID DNA-DAMAGE RESPONSE; AUTOPHAGY; QUIESCENCE; APOPTOSIS; TUMORIGENESIS; FIBROBLASTS; STARVATION; THERAPY; PATHWAY AB Withdrawal of nutrients triggers an exit from the cell division cycle, the induction of autophagy, and eventually the activation of cell death pathways. The relation, if any, among these events is not well characterized. We found that starved mouse embryonic fibroblasts lacking the essential autophagy gene product Atg7 failed to undergo cell cycle arrest. Independent of its E1-like enzymatic activity, Atg7 could bind to the tumor suppressor p53 to regulate the transcription of the gene encoding the cell cycle inhibitor p21(CDKN1A). With prolonged metabolic stress, the absence of Atg7 resulted in augmented DNA damage with increased p53-dependent apoptosis. Inhibition of the DNA damage response by deletion of the protein kinase Chk2 partially rescued postnatal lethality in Atg7(-/-) mice. Thus, when nutrients are limited, Atg7 regulates p53-dependent cell cycle and cell death pathways. C1 [Cao, Liu] China Med Univ, Key Lab Med Cell Biol, Shenyang 110001, Peoples R China. [Lee, In Hye; Kawai, Yoshichika; Fergusson, Maria M.; Rovira, Ilsa I.; Finkel, Toren] NHLBI, Ctr Mol Med, Bethesda, MD 20892 USA. [Bishop, Alexander J. R.] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA. [Motoyama, Noboru] Natl Ctr Geriatr & Gerontol, Res Inst, Dept Cognit Brain Sci, Aichi 4748511, Japan. RP Cao, L (reprint author), China Med Univ, Key Lab Med Cell Biol, Shenyang 110001, Peoples R China. EM caoliu@mail.cmu.edu; finkelt@nih.gov FU National Natural Science Foundation of China [81130042, 31171323]; NIH; Ellison Medical Foundation FX We thank M. Komatsu for providing the Atg7+/- mice. Supported by National Natural Science Foundation of China grants 81130042 and 31171323 (L.C.), NIH intramural funds, and a grant from the Ellison Medical Foundation (T.F.). NR 25 TC 100 Z9 107 U1 1 U2 47 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD APR 13 PY 2012 VL 336 IS 6078 BP 225 EP 228 DI 10.1126/science.1218395 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 924PN UT WOS:000302703900049 PM 22499945 ER PT J AU Xue, YX Luo, YX Wu, P Shi, HS Xue, LF Chen, C Zhu, WL Ding, ZB Bao, YP Shi, J Epstein, DH Shaham, Y Lu, L AF Xue, Yan-Xue Luo, Yi-Xiao Wu, Ping Shi, Hai-Shui Xue, Li-Fen Chen, Chen Zhu, Wei-Li Ding, Zeng-Bo Bao, Yan-ping Shi, Jie Epstein, David H. Shaham, Yavin Lu, Lin TI A Memory Retrieval-Extinction Procedure to Prevent Drug Craving and Relapse SO SCIENCE LA English DT Article ID LONG-TERM-MEMORY; COCAINE-SEEKING; DISRUPTING RECONSOLIDATION; BASOLATERAL AMYGDALA; PROTEIN-SYNTHESIS; FEAR MEMORIES; CUE-EXPOSURE; PKM-ZETA; MECHANISMS; ADDICTION AB Drug use and relapse involve learned associations between drug-associated environmental cues and drug effects. Extinction procedures in the clinic can suppress conditioned responses to drug cues, but the extinguished responses typically reemerge after exposure to the drug itself (reinstatement), the drug-associated environment (renewal), or the passage of time (spontaneous recovery). We describe a memory retrieval-extinction procedure that decreases conditioned drug effects and drug seeking in rat models of relapse, and drug craving in abstinent heroin addicts. In rats, daily retrieval of drug-associated memories 10 minutes or 1 hour but not 6 hours before extinction sessions attenuated drug-induced reinstatement, spontaneous recovery, and renewal of conditioned drug effects and drug seeking. In heroin addicts, retrieval of drug-associated memories 10 minutes before extinction sessions attenuated cue-induced heroin craving 1, 30, and 180 days later. The memory retrieval-extinction procedure is a promising nonpharmacological method for decreasing drug craving and relapse during abstinence. C1 [Xue, Yan-Xue; Luo, Yi-Xiao; Wu, Ping; Shi, Hai-Shui; Xue, Li-Fen; Chen, Chen; Zhu, Wei-Li; Ding, Zeng-Bo; Bao, Yan-ping; Shi, Jie; Lu, Lin] Peking Univ, Natl Inst Drug Dependence, Beijing 100191, Peoples R China. [Shi, Hai-Shui] Hebei Med Univ, Basic Med Coll, Dept Biochem & Mol Biol, Shijiazhuang 050017, Peoples R China. [Epstein, David H.; Shaham, Yavin] NIDA, Intramural Res Program, NIH, Baltimore, MD 21224 USA. RP Lu, L (reprint author), Peking Univ, Natl Inst Drug Dependence, Beijing 100191, Peoples R China. EM linlu@bjmu.edu.cn RI shaham, yavin/G-1306-2014 FU National Basic Research Program of China [2009CB522000, 2011CB707800]; Natural Science Foundation of China [91132716, 31070958]; National Institute on Drug Abuse FX This work was supported in part by the National Basic Research Program of China (No 2009CB522000 and 2011CB707800) and the Natural Science Foundation of China (No 91132716 and 31070958). The preparation of the manuscript was also supported in part by the Intramural Research Program of the National Institute on Drug Abuse. The authors declare that they do not have any conflicts of interest related to the data presented in this manuscript. NR 46 TC 165 Z9 179 U1 11 U2 119 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD APR 13 PY 2012 VL 336 IS 6078 BP 241 EP 245 DI 10.1126/science.1215070 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 924PN UT WOS:000302703900053 PM 22499948 ER PT J AU Chen, L Kasai, T Li, YG Sugii, Y Jin, GL Okada, M Vaidyanath, A Mizutani, A Satoh, A Kudoh, T Hendrix, MJC Salomon, DS Fu, L Seno, M AF Chen, Ling Kasai, Tomonari Li, Yueguang Sugii, Yuh Jin, Guoliang Okada, Masashi Vaidyanath, Arun Mizutani, Akifumi Satoh, Ayano Kudoh, Takayuki Hendrix, Mary J. C. Salomon, David S. Fu, Li Seno, Masaharu TI A Model of Cancer Stem Cells Derived from Mouse Induced Pluripotent Stem Cells SO PLOS ONE LA English DT Article ID MAMMARY MICROENVIRONMENT; TUMOR-CELLS; TUMORIGENIC PHENOTYPE; COPY NUMBER; DIFFERENTIATION; SUPPRESSES; GENERATION; EXOSOMES AB Cancer stem cells (CSCs) are capable of continuous proliferation and self-renewal and are proposed to play significant roles in oncogenesis, tumor growth, metastasis and cancer recurrence. CSCs are considered derived from normal stem cells affected by the tumor microenvironment although the mechanism of development is not clear yet. In 2007, Yamanaka's group succeeded in generating Nanog mouse induced pluripotent stem (miPS) cells, in which green fluorescent protein (GFP) has been inserted into the 5'-untranslated region of the Nanog gene. Usually, iPS cells, just like embryonic stem cells, are considered to be induced into progenitor cells, which differentiate into various normal phenotypes depending on the normal niche. We hypothesized that CSCs could be derived from Nanog miPS cells in the conditioned culture medium of cancer cell lines, which is a mimic of carcinoma microenvironment. As a result, the Nanog miPS cells treated with the conditioned medium of mouse Lewis lung carcinoma acquired characteristics of CSCs, in that they formed spheroids expressing GFP in suspension culture, and had a high tumorigenicity in Balb/c nude mice exhibiting angiogenesis in vivo. In addition, these iPS-derived CSCs had a capacity of self-renewal and expressed the marker genes, Nanog, Rex1, Eras, Esg1 and Cripto, associated with stem cell properties and an undifferentiated state. Thus we concluded that a model of CSCs was originally developed from miPS cells and proposed the conditioned culture medium of cancer cell lines might perform as niche for producing CSCs. The model of CSCs and the procedure of their establishment will help study the genetic alterations and the secreted factors in the tumor microenvironment which convert miPS cells to CSCs. Furthermore, the identification of potentially bona fide markers of CSCs, which will help the development of novel anti-cancer therapies, might be possible though the CSC model. C1 [Chen, Ling; Kasai, Tomonari; Sugii, Yuh; Jin, Guoliang; Okada, Masashi; Vaidyanath, Arun; Mizutani, Akifumi; Kudoh, Takayuki; Seno, Masaharu] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan. [Chen, Ling] Japan Soc Promot Sci, Tokyo, Japan. [Chen, Ling] Tianjin Cent Hosp Gynecol Obstet, Dept Pathol, Tianjin, Peoples R China. [Li, Yueguang] Tianjin 4th Ctr Hosp, Dept Gen Surg, Tianjin, Peoples R China. [Satoh, Ayano] Okayama Univ, Multidisciplinary Div, Okayama 7008530, Japan. [Hendrix, Mary J. C.] Northwestern Univ, Feinberg Sch Med, Childrens Mem Res Ctr, Chicago, IL 60611 USA. [Salomon, David S.] NCI, Lab Mammary Biol & Tumorigenesis, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Fu, Li] Tianjin Med Univ, State Key Lab Breast Canc Res, Dept Breast Canc Pathol & Res Lab, Canc Hosp, Tianjin, Peoples R China. RP Chen, L (reprint author), Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan. EM fulijyb@hotmail.com; mseno@cc.okayama-u.ac.jp RI SENO, Masaharu /B-2092-2011; OI SENO, Masaharu /0000-0001-8547-6259; SATOH, Ayano/0000-0003-3736-1283 FU Ministry of Education, Culture, Sports, Science and Technology of Japan [21300179]; National Natural Science Foundation of China (Key Program) [30930038] FX This work was supported by Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (No. 21300179, http://www.waseda.jp/rps/en/manual/kakenhi_honbun.html), and by National Natural Science Foundation of China (Key Program, Grant No. 30930038, http://www.nsfc.gov.cn/e_nsfc/desktop/zn/0101.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 19 Z9 25 U1 0 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 12 PY 2012 VL 7 IS 4 AR e33544 DI 10.1371/journal.pone.0033544 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TZ UT WOS:000305338600009 PM 22511923 ER PT J AU Decker, BK Perez, F Hujer, AM Hujer, KM Hall, GS Jacobs, MR Gebreyes, WA Zoll, ST Massire, C Eshoo, MW Ecker, DJ Rather, PN Bonomo, RA AF Decker, Brooke K. Perez, Federico Hujer, Andrea M. Hujer, Kristine M. Hall, Geraldine S. Jacobs, Michael R. Gebreyes, Wondwossen A. Zoll, Scott T. Massire, Christian Eshoo, Mark W. Ecker, David J. Rather, Philip N. Bonomo, Robert A. TI Longitudinal Analysis of the Temporal Evolution of Acinetobacter baumannii Strains in Ohio, USA, by Using Rapid Automated Typing Methods SO PLOS ONE LA English DT Article ID ANTIBIOTIC-RESISTANCE; MOLECULAR EPIDEMIOLOGY; GENETIC DIVERSITY; OUTBREAK; PCR; REPRODUCIBILITY; DISSEMINATION; EMERGENCE; INFECTION; MILITARY AB Genotyping methods are essential to understand the transmission dynamics of Acinetobacter baumannii. We examined the representative genotypes of A. baumannii at different time periods in select locations in Ohio, using two rapid automated typing methods: PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS), a form of multi-locus sequence typing (MLST), and repetitive-sequence-based-PCR (rep-PCR). Our analysis included 122 isolates from 4 referral hospital systems, in 2 urban areas of Ohio. These isolates were associated with outbreaks at 3 different time periods (1996, 2000 and 2005-2007). Type assignments of PCR/ESI-MS and rep-PCR were compared to each other and to worldwide (WW) clone types. The discriminatory power of each method was determined using the Simpson's index of diversity (DI). We observed that PCR/ESI-MS sequence type (ST) 14, corresponding to WW clone 3, predominated in 1996, whereas ST 12 and 14 coexisted in the intermediate period (2000) and ST 10 and 12, belonging to WW clone 2, predominated more recently in 2007. The shift from WW clone 3 to WW clone 2 was accompanied by an increase in carbapenem resistance. The DI was approximately 0.74 for PCR/ESI-MS, 0.88 for rep-PCR and 0.90 for the combination of both typing methods. We conclude that combining rapid automated typing methods such as PCR/ESI-MS and rep-PCR serves to optimally characterize the regional molecular epidemiology of A. baumannii. Our data also sheds light on the changing sequence types in an 11 year period in Northeast Ohio. C1 [Decker, Brooke K.; Perez, Federico; Hujer, Andrea M.; Hujer, Kristine M.; Bonomo, Robert A.] Case Western Reserve Univ, Sch Med, Dept Med, Cleveland, OH 44106 USA. [Perez, Federico; Hujer, Andrea M.; Hujer, Kristine M.; Bonomo, Robert A.] Vet Affairs Med Ctr, Louis Stokes Cleveland Dept, Res Serv, Cleveland, OH USA. [Hall, Geraldine S.] Cleveland Clin, Pathol & Lab Med Inst, Cleveland, OH 44106 USA. [Jacobs, Michael R.] Case Western Reserve Univ, Sch Med, Dept Pathol, Cleveland, OH 44106 USA. [Gebreyes, Wondwossen A.] Ohio State Univ, Coll Vet Med, Columbus, OH 43210 USA. [Zoll, Scott T.; Massire, Christian; Eshoo, Mark W.; Ecker, David J.] Abbott Mol Inc, Ibis Biosci Inc, Carlsbad, CA USA. [Rather, Philip N.] Vet Affairs Med Ctr, Res Serv, Decatur, GA 30033 USA. [Rather, Philip N.] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA. [Bonomo, Robert A.] Case Western Reserve Univ, Sch Med, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA. [Bonomo, Robert A.] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA. RP Decker, BK (reprint author), NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM Robert.Bonomo@va.gov OI Decker M.D., Brooke K/0000-0002-3404-9115 FU Steris Corporation; Veterans Affairs Merit Review Program; National Institute of Allergy and Infectious Diseases at the National Institutes of Health [RO1-AI072219-05]; Geriatric Research, Education and Clinical Care Center VISN [10]; Research Career Scientist Award from the Department of Veterans Affairs FX Funding for this study was provided by the Steris Corporation to FP. RAB is supported by the Veterans Affairs Merit Review Program, the National Institute of Allergy and Infectious Diseases at the National Institutes of Health (grants RO1-AI072219-05) and the Geriatric Research, Education and Clinical Care Center VISN 10. PNR is supported by a Research Career Scientist Award from the Department of Veterans Affairs. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 11 Z9 11 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 12 PY 2012 VL 7 IS 4 AR e33443 DI 10.1371/journal.pone.0033443 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TZ UT WOS:000305338600008 PM 22511922 ER PT J AU Kitahara, CM Platz, EA Ladenson, PW Mondul, AM Menke, A de Gonzalez, AB AF Kitahara, Cari M. Platz, Elizabeth A. Ladenson, Paul W. Mondul, Alison M. Menke, Andy de Gonzalez, Amy Berrington TI Body Fatness and Markers of Thyroid Function among US Men and Women SO PLOS ONE LA English DT Article ID RESTING ENERGY-EXPENDITURE; CORONARY-HEART-DISEASE; MASS INDEX; SERUM TSH; SUBCLINICAL HYPOTHYROIDISM; OBESE WOMEN; WEIGHT-LOSS; FREE T4; ABDOMINAL ADIPOSITY; EUTHYROID SUBJECTS AB Background: We evaluated the association of central versus overall adiposity on levels of thyroid stimulating hormone (TSH), free triiodothyronine (fT(3)), and free thyroxine (fT(4)) among euthyroid subjects taken from a cross-sectional, representative sample of the adult non-institutionalized U. S. population. Methods: The National Health and Nutrition Examination Survey 2007-2008 included 1,623 men and 1,491 women who were 20 years and older, with no history of thyroid or liver disease, kidney failure, diabetes, or thyroid function-altering prescription medication use (based on self-report), and having TSH, fT(3), and fT(4) levels between 0.5-4.49 mIU/L, 2.5-3.9 pg/mL, and 0.6-1.6 ng/dL, respectively. Associations between body mass index (BMI) and waist circumference (measures of overall and central adiposity, respectively) and TSH, fT(3), and fT(4) levels were estimated using multivariable linear regression models stratified by sex and adjusted for age, race, smoking status, and alcohol intake. Results: An increase in serum TSH levels was observed for every 1-quartile increase in BMI in euthyroid men (3.8% [95% CI 0.8%, 6.8%]) and euthyroid women (4.0% [95% CI 1.6%, 6.5%]). Similar, albeit slightly weaker, associations were observed with waist circumference. We also found increases in fT(3) levels with every 1-quartile increase in BMI (1.0% in men and 1.3% in women) and waist circumference (1.2% in men and 1.2% in women). No associations were observed with fT(4). Conclusions: Our results provide support that BMI and waist circumference are positively associated with levels of serum TSH and f T-3 but not fT(4) among euthyroid adults. Longitudinal studies are needed to define the temporality of these associations and their potential health implications. C1 [Kitahara, Cari M.; Platz, Elizabeth A.; Menke, Andy] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21205 USA. [Kitahara, Cari M.; Mondul, Alison M.; de Gonzalez, Amy Berrington] NCI, NIH, Div Canc Epidemiol & Genet, Rockville, MD USA. [Ladenson, Paul W.] Johns Hopkins Med Inst, Dept Med, Div Endocrinol & Metab, Baltimore, MD 21205 USA. RP Kitahara, CM (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21205 USA. EM kitaharac@mail.nih.gov RI Kitahara, Cari/R-8267-2016; OI Mondul, Alison/0000-0002-8843-1416 FU Intramural Research Program of the National Cancer Institute, National Institutes of Health FX This work was supported by the Intramural Research Program of the National Cancer Institute, National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 37 Z9 37 U1 1 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 12 PY 2012 VL 7 IS 4 AR e34979 DI 10.1371/journal.pone.0034979 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TZ UT WOS:000305338600062 PM 22511976 ER PT J AU Gaiser, T Geissinger, E Schattenberg, T Scharf, HP Durken, M Dinter, D Rosenwald, A Marx, A AF Gaiser, Timo Geissinger, Eva Schattenberg, Torsten Scharf, Hanns-Peter Duerken, Matthias Dinter, Dietmar Rosenwald, Andreas Marx, Alexander TI Case report: a unique pediatric case of a primary CD8 expressing ALK-1 positive anaplastic large cell lymphoma of skeletal muscle SO DIAGNOSTIC PATHOLOGY LA English DT Article DE ALK-1; Anaplastic large cell lymphoma; CD30; Pediatric lymphoma ID PSOAS MUSCLE; CLASSIFICATION; TRANSLOCATION; FEATURES; GENE AB Primary involvement of skeletal muscle is a very rare event in ALK-1 positive anaplastic large cell lymphoma (ALCL). We describe a case of a 10-year old boy presenting with a three week history of pain and a palpable firm swelling at the dorsal aspect of the left thigh. Histological examination of the lesion revealed a tumoral and diffuse polymorphic infiltration of the muscle by large lymphoid cells. Tumor cells displayed eccentric, lobulated "horse shoe" or "kidney-shape" nuclei. The cells showed immunohistochemical positivity for CD30, ALK-1, CD2, CD3, CD7, CD8, and Perforin. Fluorescence in situ hybridization analysis revealed a characteristic rearrangement of the ALK-1 gene in 2p23 leading to the diagnosis of ALK-1 positive ALCL. Chemotherapy according to the ALCL-99-NHL-BFM protocol was initiated and resulted in a complete remission after two cycles. This case illustrates the unusual presentation of a pediatric ALCL in soft tissue with a good response to chemotherapy. C1 [Gaiser, Timo; Marx, Alexander] Univ Heidelberg, Med Fac Mannheim, Inst Pathol, D-68167 Mannheim, Germany. [Schattenberg, Torsten; Scharf, Hanns-Peter] Univ Heidelberg, Med Fac Mannheim, Orthoped Clin, D-68167 Mannheim, Germany. [Duerken, Matthias] Univ Heidelberg, Med Fac Mannheim, Dept Pediat, D-68167 Mannheim, Germany. [Gaiser, Timo] NCI, Sect Canc Genom, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Geissinger, Eva; Rosenwald, Andreas] Univ Wurzburg, Inst Pathol, D-97080 Wurzburg, Germany. RP Gaiser, T (reprint author), Univ Heidelberg, Med Fac Mannheim, Inst Pathol, Theodor Kutzer Ufer 1-3, D-68167 Mannheim, Germany. EM timo_gaiser@web.de NR 19 TC 3 Z9 3 U1 0 U2 0 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-1596 J9 DIAGN PATHOL JI Diagn. Pathol. PD APR 12 PY 2012 VL 7 AR 38 DI 10.1186/1746-1596-7-38 PG 5 WC Pathology SC Pathology GA 949BS UT WOS:000304551000001 PM 22497840 ER EF